LOCUS       DBNHOH010000001         2494 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_705027, whole genome shotgun sequence.
ACCESSION   DBNHOH010000001 DBNHOH010000000
VERSION     DBNHOH010000001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_705027"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..505)
                     /locus_tag="AC16V7_00005"
     CDS             complement(164..505)
                     /locus_tag="AC16V7_00005"
                     /inference="COORDINATES: protein motif:HMM:NF023376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HIR6255528.1"
                     /translation="MPSSSPPRPAAPNLPPLEVPPDFPIEYANLVRIAHSPSEIVFDF
                     AQLLPGGTPVRLQSRIVMSALGAKLFFRALADNLGKYESAYGEIKIPSGSTLADQLFR
                     PPQPPENPPQR"
     gene            complement(712..1128)
                     /locus_tag="AC16V7_00010"
     CDS             complement(712..1128)
                     /locus_tag="AC16V7_00010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR6255529.1"
                     /translation="MYHTIIVVGNLGRDPEMRYTPGGSAVTNFNIATNRQYTASDGTP
                     VKETIWFRVSTWGKTAEVCNQYLRKGSKVLVEGRLIPDQASGGPRVWTRQDGTAAASF
                     EVSANTVRFLSTRGEAEGEIPFGEGEVSANDDDIPF"
     gene            complement(1492..2271)
                     /locus_tag="AC16V7_00015"
     CDS             complement(1492..2271)
                     /locus_tag="AC16V7_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255530.1"
                     /translation="MITAEDLIHLPYTPGLTSAGVLQARRSLALGLVQADNLHIAPLR
                     RLVTAVAAELSLRRHLTEAGMPFKTLQTTPFTQSDWGDVVLGGRRCHLVEILYSRRSI
                     LRHIKDQHSSILQTLACLPDVYLTSELLEHHDLLVFTIILAKPQPSWYEIRRYPEKIL
                     PCHFAYILSKAWKNPAPGRVYGQISLTNEGESEFVIELGGRGVKGQYQSEHLCLPAHQ
                     SVNVAQNYTALAYLSTETIPAVQLSLSRAEPDRPLRIPAHR"
     gene            complement(2313..>2494)
                     /locus_tag="AC16V7_00020"
     CDS             complement(2313..>2494)
                     /locus_tag="AC16V7_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255531.1"
                     /translation="LAWDLGRLLGQFYTLKDERIWCQDNQRLAPYGLGSAWVSNTFHH
                     YCLQFRNDLEISDKG"
BASE COUNT          581 a          587 c          717 g          609 t
ORIGIN      
        1 tcctgtgcat tggcgcgttc gtctcggtgg atggcgcgaa ttgcgttggc gcaatggcgc
       61 gtgagcgtac gtgcccgggt gagagcctga tcgcgcacca gtgtgcgcgt ttcgaaggat
      121 tggcggatgc gctcggcaat atcatcaagg ttttgcatag atcttacctc tggggagggt
      181 tttcgggggg ttggggcggg cggaagagtt ggtcagcaag ggttgatcca ctgggaattt
      241 tgatctctcc gtaggctgat tcgtattttc ccaggttatc tgccagtgcg cggaaaaaca
      301 attttgcacc tagggcggac ataacgatgc gggattgcag gcgaactggt gtgccgcctg
      361 gcagtagctg ggcgaaatca aaaacaatct ctgagggaga gtgagcgatg cgcaccaggt
      421 tggcatactc gatagggaaa tcgggcggca cttccaacgg aggcaaatta ggtgccgccg
      481 gtcgtggggg agaagatgaa ggcatgcgta tctcctggaa ttagtttcca ccacccttta
      541 tttcccattc tattatgcgg gtaaaggatt tgttggattt tgggcgggag caacccgccc
      601 aaaatccaaa ttatcagctt atccaattcc cgcaatgcag gggaatgggt taggggttgg
      661 ggatttggtg atcaatcaga acccgaaaaa atccattcgg ggtttgtcat actagaaggg
      721 gatatcgtca tcgttggctg aaacttcccc ttcgccgaag gggatttcgc cttcggcctc
      781 gccacgggtg gagaggaagc gcactgtgtt cgcgctgacc tcaaaggacg ctgcagcggt
      841 cccatcctgg cgcgtccaga cccgaggccc tccactggcc tgatccggta taaggcggcc
      901 ctcaaccaat actttgctgc ctttgcgcaa gtactggttg cacacctctg ctgttttacc
      961 ccaagtggag acacggaacc aaatggtttc cttaacagga gtaccatccg aagccgtata
     1021 ttgccggttg gtggcaatgt tgaagttggt gaccgctgaa ccgccaggtg tataacgcat
     1081 ctcagggtct cgacctagat ttccaacgac gataatggtg tgatacattt tggtatctcc
     1141 tttcgttcag gtgagcggtc tgtcttaagt tccggacgca caccgcccgg ggcaggccct
     1201 agattcactc accgaataag cagaacaatt gtacactaaa aacaaaaaac gcgttaggta
     1261 tttttttgcg agagttcagg attttgacta tcatgcgtta gaattgatcc aggcgaagaa
     1321 ttgcgggagg gagtgcaact ctgagatatg taaaaatttt gcaggagttt gcacgggcaa
     1381 gtcatcccag gtgcgatcag tgggggaaag tcggcgagct ttgctgtgga aatctcccac
     1441 cgtcatgtag ccagccaata tgatctcgac cccgttaagc cacaggttaa cctaacgatg
     1501 agctggaatg cgcaaaggtc gatctggttc tgctcgtgac aaagagagtt gtactgccgg
     1561 gattgtctct gtggacagat atgccagggc tgtgtaattc tgtgcaacgt tcacgctctg
     1621 gtgtgcaggc aagcaaagat gctcgctctg gtattggcct ttaacccctc gaccaccaag
     1681 ctcaatgacg aattcacttt caccttcatt ggtcagggat atttgaccat atactctccc
     1741 aggcgcagga tttttccagg cttttgacaa aatgtacgca aaatggcaag gaagaatctt
     1801 ttctggatag cggcggattt cataccagct aggctgaggc tttgctagta tgatggtgaa
     1861 aaccagcagg tcatgatgct ctagcaactc agaggttaga tacacgtctg gtaggcaagc
     1921 taaggtttgc aagatgcttg aatgctgatc ttttatatgg cgcaggattg atctgcggga
     1981 gtacaggatt tctacaagat ggcagcggcg cccgcccagt acgacatcac cccaatcgga
     2041 ttgcgtaaag ggtgtggttt ggagggtttt aaagggcatc cctgcttctg tcaggtgtcg
     2101 tcgcaaggaa agttcagccg caacagccgt gaccagcctg cgcaaggggg cgatgtgcag
     2161 gttgtccgcc tgaacgagac cgagcgccag gctgcgacgc gcctgcaaga cgccggctga
     2221 tgtcaggcct ggggtatagg gcaggtgtat gagatcttca gcagtgatca tgatgcagag
     2281 atccagcgga ctaattgtct aagaacttgc ccctatccct tatcacttat ctccaaatca
     2341 tttctaaatt gaaggcagta gtggtggaag gtgttgctca cccaggcgct tcccagaccg
     2401 tatggagcta aacgctggtt gtcttggcac cagatgcgtt cgtcttttag cgtgtagaac
     2461 tgtcccaaca ggcgacctaa atcccaggct aaag
//
LOCUS       DBNHOH010000002         2460 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_192311, whole genome shotgun sequence.
ACCESSION   DBNHOH010000002 DBNHOH010000000
VERSION     DBNHOH010000002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_192311"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="AC16V7_00025"
     CDS             complement(<1..245)
                     /locus_tag="AC16V7_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255532.1"
                     /translation="MPESVLSFQSLPQEYQQVIQLAEDRYGIAIAPLQELAGGWSGAI
                     IYLVRVASTDSKKVEHLILKLDRKRTMSSSDEVSRHK"
     gene            complement(1149..1382)
                     /locus_tag="AC16V7_00030"
     CDS             complement(1149..1382)
                     /locus_tag="AC16V7_00030"
                     /inference="COORDINATES: protein motif:HMM:NF020553.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HIR6255533.1"
                     /translation="MEITPKTKVSDILEEYGDIADVMEIFGIKRVARYSFRKMLTKAL
                     TVEVAARVHRVPLDEFLETLNNAIEMRAKNQEP"
     gene            complement(1394..1774)
                     /locus_tag="AC16V7_00035"
     CDS             complement(1394..1774)
                     /locus_tag="AC16V7_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255534.1"
                     /translation="MNLKNMFATSAIVAILLGLGLILVPVPLMTARTGHPPDVLTAHV
                     ARQFGIAMLAAGVISWMARRTTDSTARNAIVVGLALLYVLLPIETAISIVRGSESAEG
                     FVLVVVFGILAVGFILLGKPRLTF"
BASE COUNT          663 a          582 c          559 g          656 t
ORIGIN      
        1 tttttatgtc gggagacttc atcggaagac gacatcgtcc gtttgcggtc aagcttcagg
       61 attaggtgtt ccactttttt ggaatctgtg gaagcgacgc gcaccaggta aatgattgct
      121 cccgaccacc caccggccaa ttcctggagc ggggcaatgg cgatcccata tcgatcttcc
      181 gcgagctgaa taacttgttg gtattcctgg ggcagcgatt gaaaactcag tacagattcc
      241 ggcatagttc ttacctttca aacccagaat gtgcggtagt aaataacagc ttggaggatt
      301 atagcattac atttctctcc actccccaga cgaccatttg aatagggcaa tcgcatttgg
      361 gcgatttccc accactgagg agagaaaaga atgcttacca ccctatcacc gttctggatc
      421 acccattatc gaaacatccg taccacaaac aaaaactgct cgacaacatc ttgcggttct
      481 tcgtgtaacc catagcgcat gagttccgaa ccacacccat gcctctttct aagggtaata
      541 agttacgaac gtgttacatt ttcaagaatc ttggtctcaa aatcacgagt cgtcaaatta
      601 tgctggcgtt atcttttttg aattatttac taccgaaatt tgtgattgcc aaataatcaa
      661 ccgtactctg caactcattc aaaaacccag gaagatgaaa gccatcaata gtgccaatat
      721 ctttcactgc ttcaagcaat gtggaaatat cgatgtttac tgtcatgctg aatatgtttg
      781 gtactttatt gaagtggaca tcaaagtatt aaccacgagg ccaagttttc caatcgattg
      841 gtgtaaattt cataaggttt ccttaaatag cttacagttc ggcaatgctt tctgcagctt
      901 gccaaccccg gtatcggcaa ccttcgtgaa acggaggttt agcttgcgga ggttaaccag
      961 cccggcaagg tgcgtaaaac ctgtgttcac attgggcgct caggtgtagg ttcgaactgg
     1021 tcgaggaggg cgaccgcgcc agtctggact ggttcggtgg atatggcaaa ggtgcctgtc
     1081 cttgatgaag aatgcttagc cgctaggttg tcccccacat gtgatctaga gatcgcttcg
     1141 gcgccgtctc atggctcttg attcttcgca cgcatttcaa tagcgttatt cagtgtttcc
     1201 aggaattcat caagtgggac cctgtgcaca cgcgcagcca cctcaaccgt caaagctttg
     1261 gttagcatct ttcggaatga gtagcgcgcc acgcgcttga tgccaaatat ctccatcaca
     1321 tcagcgatat caccgtattc ttccaggata tcggatactt ttgttttggg tgtgatttcc
     1381 attggttgtc tctttagaag gtcaatcttg gtttgcctaa cagtatgaaa ccgacagcca
     1441 gtatcccaaa aacgactacc aatacgaagc cttcggcgct ttctgagcca cgcacaattg
     1501 agatggcagt ctcgatgggt aacagcacgt atagcaaggc taagccaact acaatagcgt
     1561 tgcgagcagt cgaatcagtt gtgcggcgcg ccatccaaga gattactcct gcggctagca
     1621 tggcaatacc aaactgcctg gcaacatgag cggtcagaac atcaggggga tgaccagtcc
     1681 ttgcagtcat taacggcacc ggtaccagga taagtcccag tccaagcagg atcgcgacaa
     1741 ttgcgctggt ggcaaacata ttcttcaggt tcataactca ttctccagtt ttcgaaatta
     1801 agagggataa agcttggtct gacacgattg gatactggtc gtcagcaggt gatctaaaat
     1861 atgttgacag cctgaactat ctggataatc tgttatccat ctttgagaaa gtcccgcaat
     1921 tatacttcat aaaaatattt actaaccatg cttcataaat gccctggttg attacaactt
     1981 catgacgctt gtaaagtttc tgctccttga tccaaaacca ctgcatcatc cgtttgtggt
     2041 cgtcgatgaa atgcccaatg cgcaactgct gtaagtccta aatgcgaagc gctgtattga
     2101 taccaaccac tatcagtaaa tgatcacggt agcgcttttt ccgcaggctt accccccaca
     2161 tataggtgac ccgtagcgga tgagttccga accacccctt ccctagaatt aggggaaaac
     2221 acagtgcgaa gtgaggaatc ctaatatagg tctgctaagt cacaagatga acatcagccc
     2281 tgtagagacc agctgatttg gaataactca atcatctaga tcgtagatcg gaaatctatc
     2341 tgtggtgcgg gaaaaaacag gtctctggtc atattaatca aatcaccttt ttcggtatac
     2401 taataataac tgtaggactt acgcagttga aggggaaaga gggggagttt gggggggggg
//
LOCUS       DBNHOH010000003         1179 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1153723, whole genome shotgun sequence.
ACCESSION   DBNHOH010000003 DBNHOH010000000
VERSION     DBNHOH010000003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1153723"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="AC16V7_00040"
     CDS             complement(<1..1030)
                     /locus_tag="AC16V7_00040"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR6255535.1"
                     /translation="MNAVHCINCHLQYPDTDIPYRCPRCGGLFDFEALWSFDPSQVDS
                     GQPGIWRYRHTFGLPDGAPTISLGEGNTPLLWAQAFGKKVAFKCEYLNPSGSFKDRGS
                     VPLVSFIRSRGVELAVEDSSGNAGASFAAYAARAGLKAQVYIPDSASGPKRRQIEAYG
                     AEVVRIMGPRSNAAEAVRRVADQGAVYASHAYLPFNLPGYATIAYEIVEQLNGAPGTL
                     ITPVGQGGLLLGVARGFQALQEAGTISKMPVLIGVQARACAPLWALYNYGSTGLGWSV
                     EGQTLAEGIRVFPPLRGDSVLRAMTANQGIFLAVDEEDILPGREALAHLGFYVEPTSA
                     VIWSALEQL"
BASE COUNT          310 a          347 c          259 g          263 t
ORIGIN      
        1 ccaattgttc caacgcgctc caaattaccg ctgacgttgg ctccacataa aaacctaaat
       61 gagccaatgc ctccctaccg ggcagtatat cctcctcatc cacagcgaga aaaattcctt
      121 ggttagcagt catggcgcga agcacagaat cgccacgtaa tggaggaaag acacgaatgc
      181 cctccgccag ggtctgacct tcaacagacc agcccaaacc agtcgatcca taattataca
      241 aagcccacag gggagcgcaa gcgcgtgcct gcacacctat caaaactggc attttgctga
      301 ttgtccctgc ctcttgcaag gcttggaatc cacgcgctac acccagaagc aagccgcctt
      361 gccccaccgg ggtaatcaag gtgccaggcg ctccatttag ttgctccact atctcatagg
      421 cgattgtggc atagccaggc agattgaatg gcaagtaggc gtgactggcg tacacagcac
      481 cttgatctgc aacacgacga acagcctctg ctgcattaga tcgcggcccc atgatacgta
      541 ccacctcagc accgtaggct tcaatttgac ggcgtttcgg acctgatgca gaatctggga
      601 tataaacttg agcttttaaa cccgcacggg cagcataagc agcgaacgat gctcctgcgt
      661 tcccagaaga atcctcaact gccaattcta ctcctcgaga gcgaatgaag ctaaccaatg
      721 gaacactacc gcggtctttg aatgatccag atggatttag gtattcacat ttaaaggcca
      781 cttttttacc aaaagcttgc gcccaaagca atggcgtgtt tccttctccc agagaaattg
      841 tgggcgcgcc atcgggaagc ccaaatgtat gccggtatcg ccaaatccct ggttgccctg
      901 aatccacctg agaagggtca aatgaccata atgcttcgaa gtcaaacaaa ccaccgcagc
      961 gcgggcagcg atagggtata tctgtatccg gatattgaag atgacaattt atgcaatgga
     1021 ccgcattcat ccgctatcta ttacccctgc gaatcccagg tatcgtctcg ctctggtgta
     1081 tactcacctt ccacccagac ctccccagca ggtcctactt cttttttcca taccgggacg
     1141 atctctttaa ggcgatcaat tccataccga gctgcctca
//
LOCUS       DBNHOH010000004         2882 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1346002, whole genome shotgun sequence.
ACCESSION   DBNHOH010000004 DBNHOH010000000
VERSION     DBNHOH010000004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1346002"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="AC16V7_00045"
     CDS             <1..261
                     /locus_tag="AC16V7_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HIR6255536.1"
                     /translation="YTDVEVNLYQAVLGSEVTVPTLSGNVVLTIPPGTQPGQTFRLIG
                     QGMPQVKNPENRGDLFVRVKVKIPRNLSAEQRELFQKLARQA"
     gene            390..1550
                     /locus_tag="AC16V7_00050"
     CDS             390..1550
                     /locus_tag="AC16V7_00050"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR6255537.1"
                     /translation="MGRLVFIVFILVLSTLACRAASIPEEKVVAPTSPAPRLDAITTP
                     IFIPPAADMVALQDTLVEMYQQVNPGVVALRALTSDGGSLGSGFVIDQDGHIITNYHV
                     VEAVNRLEVTFASGHKTRGEILGTDLDSDIAVIKVDVPKEELYPLTLSDSDQVQVGQT
                     VIAIGNPFGFSGTMTMGIVSGLSRTMLSLHEAPGGGMFSTGDIIQTDAAINPGNSGGP
                     LLNLNGEVIGVNRAIFSTQTNELNQPVNSGIGFAVSINMVKRVYPALIAEGKFDYPYV
                     GIASLSDLSLLQQETLGLSRSTGVYITEIVSGSPADQAGLRAGSRRTSIPGLLAGGDL
                     IIAIDQKEVINFSDFLSYLLRNKSPGDTVAMTILREDKEIEIELTLEKRPSR"
     gene            1596..2696
                     /locus_tag="AC16V7_00055"
     CDS             1596..2696
                     /locus_tag="AC16V7_00055"
                     /inference="COORDINATES: protein motif:HMM:NF037429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baeRF10 domain-containing protein"
                     /protein_id="HIR6255538.1"
                     /translation="MLTENELRELLDYQASAPVLSIYLNVEPSEGSVDAHKLRLRSML
                     RDVELPDDVQRVELFVEHEFNWSGRSVSIFSCMADDFFRSYTLAVPIRSRVRVSDKPY
                     VKPLADLLDAYGGYGVALVDKQGARLFYFHLGELREQEGVMGEAVRKTKRGGGSQAQG
                     RRGGTAGQTNYVEEVADRNIKDAADFAAHFFHENNVRRILLGGTEDNLTPFQHHLPKA
                     WRSLVVGTFPVSMTASHSEVLERAMQIGQEAERRREATLVKTAITGAAKGRGGVIHLE
                     ETLHAVHQGRVQTLLISDGFRAPGRRCKGCGYITSKKFQVCPFCGGKFDPIPDAVELV
                     VRKVMQSGGDVEVLHDAQALSEQGNIGGLLRY"
BASE COUNT          704 a          744 c          805 g          629 t
ORIGIN      
        1 tacacggatg tggaggtaaa tttatatcaa gctgtactgg ggagcgaggt caccgtgcca
       61 acattgagcg gcaacgtggt attaaccatc ccccctggca cacaaccagg acagactttc
      121 cggttaatcg ggcaaggcat gccccaggtc aagaatcccg aaaaccgcgg cgacttattc
      181 gtgcgagtaa aagtcaagat cccacgcaat ctctcagcag agcagcgcga gctgttccaa
      241 aagttggctc gccaggccta gaacactggc ggcaatggga agacgctctt cgcaaggtgg
      301 gtttaaatac ccgccgagga atttaagaat gggcgtatca acagattgca cgtaagggag
      361 gctaatttag gagtagtttg atgcatcgaa tgggtagact agtttttatt gtattcatct
      421 tggttctctc aacgctggct tgccgggcgg cgagcatccc ggaagagaaa gttgtcgccc
      481 ccacatcccc tgcgccccga ctcgatgcta tcactacacc catcttcatc ccacctgctg
      541 ccgatatggt tgcattacaa gacacgctgg ttgagatgta tcagcaggtt aacccgggtg
      601 tcgtcgcgct gcgcgcactc accagcgacg gcggcagctt gggctcgggc tttgtgatcg
      661 atcaagatgg gcacatcatc accaactatc acgtggttga agcagtgaac aggctggagg
      721 tgacttttgc ctctgggcat aaaacgcgcg gcgaaatcct gggtacagac ctagactcag
      781 atatcgcggt cataaaagtc gacgtgccca aggaagaact ctatccgctt accttgagcg
      841 attccgacca ggtgcaagta gggcaaacgg ttattgctat cggtaatcca tttggtttca
      901 gcggtaccat gaccatgggg atagtttctg gattaagccg caccatgtta tccctgcatg
      961 aagcccccgg tggtggcatg ttctccaccg gagatatcat ccagaccgac gccgccatca
     1021 atccgggaaa ttctggtggg ccgctactca acttaaatgg ggaggtgatc ggcgtaaacc
     1081 gggcgatctt cagcacccag accaacgagc tgaaccagcc ggtaaactct ggtattggat
     1141 ttgcggtttc cataaatatg gtcaaacgcg tctacccagc cctgatcgcc gaaggcaagt
     1201 ttgattatcc ttacgtgggt attgccagct tgagcgatct atctctgctg cagcaggaaa
     1261 ccctagggtt atctcgctcc acaggggtgt atattaccga gatcgtctcc ggcagccccg
     1321 ccgaccaagc cgggctgcga gcgggcagtc ggcgtacaag catcccaggg ctgctggccg
     1381 gtggagatct gattatagcc atcgatcaga aagaggtaat aaacttttcg gattttctga
     1441 gctacctttt aagaaataaa agccccggcg acacagtcgc catgaccatc ctacgcgaag
     1501 ataaggagat cgaaatagaa cttaccctag aaaaacggcc ctcacgttag attccatcgc
     1561 taacaattcg cccaaaccat atacaggagg ttcatatgct aactgaaaat gagctacgcg
     1621 aactgcttga ctatcaggcc agtgcgccag ttcttagcat ctatctaaat gtggagccat
     1681 ctgaaggcag tgtagacgcc cataagctac gtcttcgtag catgctaaga gatgtcgaat
     1741 tgccagacga tgtccagaga gtggagcttt ttgttgaaca tgagtttaac tggtcggggc
     1801 ggagcgtgtc gatattttcc tgtatggccg atgatttctt tcgcagttac accctggcag
     1861 tgccgatacg cagccgggta cgtgtcagtg acaaaccgta tgttaaaccg ttggctgatc
     1921 tactagatgc gtatggaggt tatggcgttg ctttagttga caaacaaggg gcgcgcctgt
     1981 tttattttca cctgggagaa ctgcgcgaac aagaaggggt tatgggggaa gctgtgcgca
     2041 agacaaagcg cgggggcggc tcccaggccc aagggcggcg ggggggaaca gcaggacaga
     2101 ccaactatgt agaggaagtg gcagacagga atatcaagga tgctgccgat ttcgcggcgc
     2161 acttttttca cgagaacaat gtgcgccgca tccttttggg tggcaccgag gataacctga
     2221 cgcccttcca gcaccacctc ccgaaagcct ggcgaagcct ggttgtgggg actttccccg
     2281 tgagtatgac cgccagccac agcgaggtac tggaacgcgc catgcagatt ggacaggagg
     2341 cagagcggcg gcgcgaagcc acgttggtga aaacagccat taccggcgct gcaaagggtc
     2401 gtggcggtgt gatccacctg gaagagacgc ttcatgcagt ccaccagggg cgagtgcaga
     2461 ccttgctcat cagcgatggc ttccgcgctc cagggcggcg ctgcaagggt tgcggctaca
     2521 tcacgtcaaa gaagttccag gtctgcccat tctgtggcgg aaaattcgac ccaattcccg
     2581 atgcagtgga attggtagtt cgcaaggtga tgcaatccgg cggagacgta gaggtgctcc
     2641 acgacgcaca ggccctgagc gaacagggga atattggagg tttactgcgc tattgatccc
     2701 aagattgccc aaaaagattg aagcgggcca taagcagccc gcttcaatct ttttaaggct
     2761 tggcgaagta tgcgttctcc agctctaata gcccggtcgc cagtacatcc aggtcagagt
     2821 tggctgcgta ggcattctcc ccaatctcct tgacagccaa atccaggcga gcttgcatat
     2881 ct
//
LOCUS       DBNHOH010000005         1446 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_769216, whole genome shotgun sequence.
ACCESSION   DBNHOH010000005 DBNHOH010000000
VERSION     DBNHOH010000005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1446)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_769216"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /gene="truB"
                     /locus_tag="AC16V7_00060"
     CDS             <1..897
                     /gene="truB"
                     /locus_tag="AC16V7_00060"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR6255539.1"
                     /translation="VKNVVSGVLVVDKPIGLTSHDVVQIIRRGTGIRRAGHTGTLDPR
                     ASGVLVVLVGPAVRLSEYVSASDKRYQATIRLGTATDTYDADGTFTTPTISVDDITED
                     LFTEILEKFIGTIEQVPPPYSAVKVKGRKAYEMARQGEEVDLEPRTIQVYSLEVLEWA
                     PPEVVIDVYCSSGTYVRSLAHDLGRELGCGAYLIGLRRTKSGRFTLRDAVPMRRLQEA
                     FTAGDWYRHLIPAAEALADWPMVELDADQVELVRHGHRVAADPGSKGWARGVSQQGDL
                     VALLEIDPETLEWQPKKVFFQS"
     gene            899..>1446
                     /locus_tag="AC16V7_00065"
     CDS             899..>1446
                     /locus_tag="AC16V7_00065"
                     /inference="COORDINATES: protein motif:HMM:NF018299.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255540.1"
                     /translation="MRHYRLLDGVSLKDVWLTIGTFDGVHLGHQEIIGRVVDGARQSG
                     VPAVAVTFYPPPTVVLKKRSDPSYLTFPEQRAQLLGELGIDVVITHPFDMQVAAMSAR
                     EFVASLHAHLHMKNLCIGYDFALGHDREGNQAVLHQLGDEFGYSLQVVPPVQVDGKVV
                     SSSLIRAHLAEGEVEKAARLLGR"
BASE COUNT          314 a          338 c          431 g          363 t
ORIGIN      
        1 gtaaaaaatg ttgtatcggg tgtattggtg gtggacaagc ccattgggct cacatctcat
       61 gatgtagtgc agatcatccg cagggggact ggtatccggc gcgcgggaca tacagggacg
      121 ttagacccac gtgcatctgg tgtgcttgtg gtcttggttg gccccgcggt tcgactcagc
      181 gagtatgttt cagcatctga caagcgctac caggcgacga ttcggttggg tactgcaacg
      241 gatacctatg acgcagatgg gacattcacc acacccacaa tatcggtgga tgacattacg
      301 gaggatctct ttaccgaaat tttggagaaa ttcatcggca ccattgaaca ggttccgcct
      361 ccttattctg ccgtaaaagt caaaggacgc aaggcgtacg agatggcgcg ccaaggagaa
      421 gaggtagacc ttgaaccgcg tacaatccag gtatacagtc tggaagtgtt ggaatgggcg
      481 ccacctgagg tcgttatcga cgtatattgc tcgtcgggga cttatgtgcg ctcactagca
      541 catgacctgg gtcgtgagct gggttgtggt gcttatttga tcgggttgcg gcggacaaaa
      601 agcggacgtt tcaccctacg ggatgcagtc cctatgcgac gtctgcagga agcattcact
      661 gctggcgatt ggtatcgtca cctgatcccc gctgcagaag cactggctga ttggcctatg
      721 gtcgagttgg atgccgacca ggtcgaactg gtccggcatg gtcatcgcgt agcggcagat
      781 cctggcagca aaggctgggc acgcggggtt agccagcagg gcgatttggt ggcattgctt
      841 gagattgatc cagaaacatt ggaatggcag cctaagaagg ttttcttcca gtcatgatat
      901 gcgacattat cgtttattgg atggcgtctc cttaaaagat gtctggctaa cgattggtac
      961 gttcgatggt gtccatctcg gccaccaaga gatcatcggc agggttgttg atggagcccg
     1021 gcaatccgga gtcccggcgg tggcggttac tttttatccc ccaccaactg tggtgctgaa
     1081 aaagcgctcc gatccctcct atctaacatt tcctgaacag cgcgcccagc ttctaggaga
     1141 actgggtata gatgtggtta tcacccatcc atttgatatg caggtggctg ccatgtcggc
     1201 gcgcgaattt gtagcttctt tgcatgcgca cctccatatg aagaatctgt gtattggcta
     1261 tgattttgcc cttgggcatg atcgggaggg taaccaggct gtcttgcatc aattgggaga
     1321 tgagtttggt tactcgctcc aggtggttcc accagtgcag gttgatggca aagtggtgtc
     1381 ttccagtttg atccgcgccc atctggctga aggagaagtg gagaaggcag cacgtttgtt
     1441 aggacg
//
LOCUS       DBNHOH010000006         1753 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_320643, whole genome shotgun sequence.
ACCESSION   DBNHOH010000006 DBNHOH010000000
VERSION     DBNHOH010000006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_320643"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1309)
                     /locus_tag="AC16V7_00070"
     CDS             complement(<1..1309)
                     /locus_tag="AC16V7_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012263.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HIR6255541.1"
                     /translation="MAIVPPPTIRAAEISTPTPTPSVSSKLQRPVMPDDPLLADKGSL
                     SYWLICLACHGDKGQGLTDEFRREIFGEDMNCWQSKCHASNHPQEGFDLPRVVPAIVG
                     PGTLSRFLTAEDLNQYILSAMPWWNPGSLSSNEAWELTAYILRENGSLPQNVEFIPQT
                     ASSAPVHLPIRSRTNEQSWQWVFLISMTLVAGALIAGSVVKSGATAAQPVKGATRPSF
                     MHHLHPPSIPLPQARWRYTLGAGGLAIYLSLVLGVTGILLMFFYIPTPEHAGPSIQTI
                     TFLVPFGSLIRGIHYWAAQALVIVALIHLLRVIFTGSFVTPRRFNFFIGLGLLVLTLF
                     LDFTGYVLRWDEGIRWALTVGTNLLQTIPLIGTQVYGFVIGGDKPGLAALTRFYAWHI
                     FGLTLVMVVLIAWHIFRVRRDGGISAPSPEQRGDTRRISRFELV"
BASE COUNT          454 a          404 c          495 g          400 t
ORIGIN      
        1 gtaccagctc aaatcggctt atgcgccggg tatcaccgcg ttgctcgggt gaaggcgctg
       61 agatgccgcc atcgcgccgc acgcggaaga tgtgccaggc aatcaataca accatcacca
      121 aggtgagacc aaagatgtgc caggcgtaga atcgggtcaa agctgccaaa cccggcttgt
      181 cgcccccaat gacaaatccg taaacctggg ttccgatgag tgggatggtt tgcaaaaggt
      241 tggtgcctac tgttaaagcc cagcggatgc cttcatccca gcggaggaca tagccggtaa
      301 agtcaagaaa cagggtcaag acgagcagtc ccaacccgat gaaaaagttg aagcggcgtg
      361 gggtgacgaa tgatccggta aatatcacgc gcaatagatg gatgagggct acgataacaa
      421 gcgcctgtgc tgcccaatag tggatgcccc ggatcaggct gccgaagggc accaggaatg
      481 tgatggtctg gatggaaggc cccgcatgct caggggttgg gatgtagaaa aacattaaca
      541 gaatgccggt gacacccagc accaatgaca gatagatggc gagaccgcct gcgccaagcg
      601 tgtatcgcca gcgagcctgt ggtaacggga tcgagggagg gtgcaagtgg tgcatgaaac
      661 ttggacgcgt tgcgccctta accggttgcg ctgcagtggc tcccgactta acaacacttc
      721 cggctatgag cgcacccgct acaagtgtca tagaaatcaa aaagacccat tgccagctct
      781 gttcgtttgt gcgcgaacgg atagggaggt gcaccggtgc tgaggatgca gtctgtggga
      841 tgaattcgac attttggggg agagagccgt tttcacgcag gatatatgcg gttagctccc
      901 aggcttcgtt tgacgacaga gaacctgggt tccaccaggg cattgcgctg agaatatatt
      961 gatttagatc ttcagcggtt aggaatcgac tcagcgtgcc cggtccgaca atggctggaa
     1021 ccacccgtgg caaatcaaat ccttcttgtg gatggttgct ggcatggcat ttcgactgcc
     1081 aacaattcat atcttcgcca aagatttcac gccggaattc atcggtcaga ccctgtcctt
     1141 tatctccgtg gcaggccagg cagattaacc agtagctcaa agagcccttg tcggccagga
     1201 gcggatcatc cggcatgaca ggtctttgca attttgagga aaccgatggt gttggagtag
     1261 gggtgcttat ctctgcagcg cggatggtag gaggggggac tatagccatc cagaccacaa
     1321 gagccaggat aaataaaatg aatagagaac ctgcaactag cgtacggtac tttggattat
     1381 tgggagaagg ggatgaatta cgaaagaaca cgttgttttt aacagtccgg tgctgtggtt
     1441 gcttcgaaat ccgctgttat tttgaatact cccaaattga tgataatgtt catctccctt
     1501 cgatatttac cgattttcat actgtggctc gaatcagtct ggagaggtaa ataatactac
     1561 agggtaattg cgtatgccct gagtggcatg cgcaatcact ctcttcagtg gtgggaaatc
     1621 gtcctcatgc gattgcccta aataatacta tactataagt gctgatcgat tattatttca
     1681 ttataatagg agttcggctg cacaagaaat atatgctgtg cgaaagcaag cgcgtttcaa
     1741 tcgggaggtg att
//
LOCUS       DBNHOH010000007         8036 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_448851, whole genome shotgun sequence.
ACCESSION   DBNHOH010000007 DBNHOH010000000
VERSION     DBNHOH010000007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8036)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8036)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8036
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_448851"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="AC16V7_00075"
     CDS             <1..399
                     /locus_tag="AC16V7_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR6255542.1"
                     /translation="TSHLHSWSVELTRPVTKEEVLDAFRAAPRIAFLRAGEGIVALNS
                     TLEMMADLGRPRGDMWEVGLWEDSLTVKGDEVFYNYQVYNQAIVVPETIDAIRAITGI
                     EKDGAASIAKTDRAMGLVREFVRRTDRLAV"
     gene            389..1399
                     /locus_tag="AC16V7_00080"
     CDS             389..1399
                     /locus_tag="AC16V7_00080"
                     /inference="COORDINATES: protein motif:HMM:NF009498.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose 1,6-diphosphate aldolase"
                     /protein_id="HIR6255543.1"
                     /translation="MLCEMKNQLTIGKLRGLQQISDARGIFAMCAMDHRGSMQRMISK
                     AESKKVAYETLAEYKRDLAEYLAPESTGILLDPIYGAAQSIAFGALPGGVGLLVSLEE
                     TGYESDAQGRVTTLLPDWSVEKIKRMGASAVKMLLYYRPDLTENAARQREVTRQVSAE
                     CLSWDIPFLIEPIAYAVSEAELDPSTFAARKTSLVVDSARDLTQLGVDVLKAEFPADM
                     RFERDEDKLWAACELLDASSVSPWILLSAGVSFDEFARQVKIACQAGASGFLGGRAIW
                     EEAMHITDRGERRQWLVTVGRDRMRRLGDIAGEYGRPWWKKWAGSMSELMDVSPEWYR
                     RY"
     gene            1424..1885
                     /locus_tag="AC16V7_00085"
     CDS             1424..1885
                     /locus_tag="AC16V7_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255544.1"
                     /translation="MKVRSWYGLTGEIEVENELWHLVRVGGVALKHPPIINLLLRRGL
                     PREDRLYLSFLHEFGHLQTLLVALIHGTLMLFARRRHGRGSLRSLTWKWQALIAHQAV
                     WELAAESYTAYYARPRYRQIYRRYPNPLGRSIFWIGMLGLAVYFTQRAVNS"
     gene            2033..2455
                     /locus_tag="AC16V7_00090"
     CDS             2033..2455
                     /locus_tag="AC16V7_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255545.1"
                     /translation="MDTQHPSKLSDPFFSLIVFSIYSGFMGAVLLLAPRIILPAFQVH
                     EEVNSFTYMLGFVLIASSFYYFASGVGRDRFFAKLTIYTRIASPIVTIILYITGNVPV
                     NYVLLSILDASGGVWTFYALRKTRSGDSLKPLGYAQRV"
     gene            2697..3485
                     /locus_tag="AC16V7_00095"
     CDS             2697..3485
                     /locus_tag="AC16V7_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR6255546.1"
                     /translation="MTTEDFIIDLFCPVGDQRPDTKKHSQANHYPSEVVTLAILFALK
                     GMGNRAFYRWLERDYGHLFPDLPGRIRLFRLFNTRRHWTQRFMAETSPNGLIDTYGIE
                     LLHPRREGRSRRQIGKKGLSNRRWIVGGKLCFVLNHLGLIVDWGVATANVYDGSAYQQ
                     VVERNAAQMVLLRDEGCVKKDWHPENLKICQRGQWNTRMIAETVLSRLTLVCHFKKVM
                     HRVWAYFQSRLAYTLAMFNLLVQWDGIQTDEDGCVQFSLAPFSL"
     gene            3587..4093
                     /locus_tag="AC16V7_00100"
     CDS             3587..4093
                     /locus_tag="AC16V7_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255547.1"
                     /translation="MMQQRIGNIAVALVLILLGVWFLSIQLFPGLRDFAYNRFTWPVA
                     VIGAGVLMGVIGLVTWVPGLLIPACIVGGIGGLLYWQNATGNWDSWAYAWTLIPGFAG
                     VGVILSGLMSRDRRALVGGGWAIFNSLVLLAIFGSFLGGMAISRYWPVLLILLGVLLL
                     AQGLFRRR"
     gene            4112..4864
                     /locus_tag="AC16V7_00105"
     CDS             4112..4864
                     /locus_tag="AC16V7_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006098724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HIR6255548.1"
                     /translation="MAKQAHFRFYAELNDLLGKPEVEVQVALSQNGDQSVKHMIESLG
                     VPHTEVGRVLVNGLAVDFSYLVQEGDRVEIYPLACQEPPTPSAIPRFILDNHLGRLAV
                     YLRMLGFDAMYRNDFQDDELASLCDREDRILLTRDKRLLMRNQVTRGYWLRSKIPRRQ
                     LEEVVRRYRLAGAIQPFQRCMRCNEPLVPVHKDVILHRLEPLTRRYFNEFHLCPTCDK
                     VYWKGSHYERMRLLIEQVKIHAIEGKDTFPTA"
     gene            4940..5629
                     /locus_tag="AC16V7_00110"
     CDS             4940..5629
                     /locus_tag="AC16V7_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6255549.1"
                     /translation="MSSSRILVVEDEPSIAEVTSLYLKRAGFQVVVATDGEEALDELG
                     RQFPDLIVLDLMLPGMDGMEITRHVRAMSDVPIIMLTARREEADRIAGLETGADDYVV
                     KPFSPQELVSRVRAVLRRSQGQSQVSNERPLVFGDLVIDPQTRLVTVQGVEVLLTVKE
                     FDMLWLLARHPRQVFSRRQLLERVWGLSDYIDPGTVTVHVRRLREKIEADPSSPAHIL
                     TMWGVGYKFEP"
     gene            5639..6910
                     /locus_tag="AC16V7_00115"
     CDS             5639..6910
                     /locus_tag="AC16V7_00115"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6255550.1"
                     /translation="MSTGSLSRVNLPVRFIIGVALIVALSLGIFNLLMRPAVNEIGLM
                     AGFLSITALVSALAGYGLYRLGWMDRSPTILWSLLGIYALSSVLTFLNVWLTARLMFA
                     SQHDLLLATVLLIFAGGIAMALGYFLSSTLTDRIRLIDQAAKAIAAGDLDVRIPVRGR
                     DEMALLARTFNQMAEQLQAAARKQQELENLRRDLIAWAGHDLQTPLTSIQAVLEALAD
                     GVVDDAETIQRYLITAQRNIQALSVLVDDLFQMAQLDAGGLPLDRAYNSLSDLISDTL
                     ESFSELAARQGVDLQGSVDPGVDLVYMDAQRIGRVFNNLLNNALRYTPQGGRVQVQAR
                     READAILVEVIDTGQGVQPDDLPFIFERFYRGEKSRSRSTGGSGLGLAIARGVVEAHG
                     GQIGVESSPQGTRFFFTIPLKDIRRDNNVRR"
     gene            6897..>8036
                     /locus_tag="AC16V7_00120"
     CDS             6897..>8036
                     /locus_tag="AC16V7_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013015000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR6255551.1"
                     /translation="MSGVDFLSELYPPDRLLTRPAQLAPYESDALTALRARPKAVVLP
                     ETQGEVIETVRLCYRNSVPFVARGSGTSLSGGSTPVAEGVVIALNRLNRMIRLDPDQR
                     LAVVEPGMINQDVSRAAAPYGLYYAPDPSSQPICTIGGNLAFNAGGAHCLKYGMTSNH
                     VLGIKAVLADGEVVQLGGDSLEGSGPDLVGLFVGSEGLFGVALEVTLRLLPKPEVYRT
                     ILAAYRSLQAAGQTVSQIVASGLLPGAMEIMDNLAIQAAEAAVSAGYPLDAASLLIVE
                     LDGEAASVSADYLKLMEIIRASDPYEVHEAKDDAERLRIWKGRKSAFSAVGRLSSDYL
                     VQDGVVPRSRLGEALAEIQRLSAQYNLRVANVFHAGDGNLHPLILY"
BASE COUNT         1660 a         2080 c         2363 g         1933 t
ORIGIN      
        1 accagccacc tgcattcttg gagcgtggag ctaacccggc cggtcacgaa ggaggaggtg
       61 ctggacgcgt ttcgcgcggc cccacgcatc gccttcctgc gcgccggtga gggcatcgtg
      121 gcgctcaaca gcacgctgga gatgatggcc gaccttggga gaccacgggg cgatatgtgg
      181 gaagtgggct tgtgggagga cagcctgacg gtgaaagggg atgaagtttt ctacaactac
      241 caggtttaca atcaagccat cgtcgtcccg gagacgatcg acgccatccg ggcgataacc
      301 ggcatcgaaa aagacggcgc ggcgtccatc gccaaaaccg accgggcgat gggcctggtg
      361 cgcgagttcg tgcgacgaac ggacaggctt gctgtgtgag atgaagaacc agctcacgat
      421 tggtaagttg cgcggcctgc agcagatttc ggatgcgcgt ggtatcttcg ccatgtgcgc
      481 catggaccac cgcggctcga tgcaacgcat gattagcaag gccgaatcta aaaaagtcgc
      541 ctatgagaca ttggcggaat acaagcgtga ccttgccgag tacctggctc ctgagtctac
      601 agggatcctg ctcgatccaa tctacggggc ggcgcagtct attgcctttg gtgcgctgcc
      661 cgggggtgtc ggcttgttgg tcagcctgga agaaactggc tacgagagcg acgctcaagg
      721 ccgggtgacc acgctgctgc cggactggag cgtggagaaa atcaagcgca tgggcgcttc
      781 tgcggtcaag atgctgctat actatcgccc cgacctgacc gagaatgccg cccgccagcg
      841 cgaagtcact cgtcaggtca gcgccgaatg tttgtcttgg gacattccgt tcttgatcga
      901 gccaatcgcc tatgcggtct ctgaagctga actagatcca tccacctttg ccgcccggaa
      961 gacgagcctg gtagtcgatt cggcgcgtga cctgacgcaa ctgggcgtag atgtgctgaa
     1021 agccgaattt cctgccgaca tgcgcttcga gcgggatgag gacaagctgt gggcagcctg
     1081 tgaactgctc gatgcaagct cggtatcgcc gtggattttg ttgagcgccg gggtaagctt
     1141 cgacgagttc gcccggcagg tgaagatcgc ctgccaagct ggggcttcgg gcttcctggg
     1201 cggccgcgcc atttgggaag aagcaatgca tatcaccgac cggggagaac gccgtcaatg
     1261 gctggttact gtagggcgcg atcgcatgcg ccgcttgggc gacatcgccg gtgaatacgg
     1321 ccggccgtgg tggaagaaat gggctggctc aatgagcgag ctgatggatg tctcacccga
     1381 gtggtatcgc cgttattgat ggctacggct atggcgaggt gagatgaagg ttcgctcgtg
     1441 gtatggcctc accggcgaga tagaggtcga gaacgagctc tggcacctgg tcagagtcgg
     1501 gggagtggct ttgaaacacc ctcctatcat taacttgctc ctacggcgag gcctacctcg
     1561 cgaggaccgc ctgtacctta gctttctgca cgagtttggt cacctgcaga ccctcctggt
     1621 cgctctgatt catggcactc taatgctttt tgcaaggcgt aggcacggca gaggaagctt
     1681 gagatcatta acttggaaat ggcaagctct catcgctcac caggctgtat gggaattggc
     1741 agccgagtct tacacggcgt actatgccag acctaggtat cgccagattt atcggcggta
     1801 tcccaatccc cttggtcggt ctattttttg gattgggatg ttaggtttgg cagtatattt
     1861 tacacaacgc gcagtgaatt cttgaaggca atactctctc ataagagtgg tcgttgatac
     1921 aagcaaattg gctggggaaa aaacacgcca aacggcgcta tacactatag caatcgaacc
     1981 ttatactctc aataattgaa aatgaaatat ttccattcaa caggaggtcc tcgtggatac
     2041 acaacatcca agtaagttgt ctgacccatt ctttagtttg attgtgttta gcatctactc
     2101 cgggttcatg ggagcggtgc ttctgctcgc accccggatc atccttcctg cgtttcaggt
     2161 gcacgaagag gtgaattctt ttacctatat gcttgggttt gtcttgatag cctcgtcgtt
     2221 ctactatttt gcttccggcg tcgggaggga cagattcttc gctaaactta ccatatatac
     2281 ccgaattgct tcccctattg taacaatcat cctgtacatt actgggaacg tgccggtcaa
     2341 ttatgtactc ctcagtatcc tggatgcctc aggcggagtc tggacgtttt atgccctcag
     2401 aaagacccgt tctggggata gcctcaaacc gctagggtat gcacaacggg tataatccat
     2461 gggggggcgg gcaatgaaag caaatgccgc caaagaatag ctcggattac gtgccagggc
     2521 atcactcaga gcgagccggg ttgtaaagta acggcgctct tagcagccta cgctggtcag
     2581 catcccattg tgctgaccag cattttgtat catcttgtta aaccaatggt gctagtttga
     2641 aaaaatcatg aggtacactg ctccaagtgt ccatcagact caaggagtgt gccctcatga
     2701 ccaccgaaga ttttatcatt gacctgtttt gccccgtggg tgatcaaagg cccgatacca
     2761 agaagcattc ccaagccaat cattatccca gcgaagtggt cacgcttgcc attctgttcg
     2821 ctctcaaagg gatgggcaat cgcgcctttt atcgctggct ggagcgagat tacgggcatt
     2881 tgttccctga tctgccaggc cgcatccgcc tgtttcggct gttcaatacc cgtcggcatt
     2941 ggacgcagcg ctttatggct gaaacttccc ccaatggttt gatcgacacc tatggcattg
     3001 aactgcttca cccgcgccgg gaaggccgca gcaggcggca aattggcaag aaagggctgt
     3061 ccaaccggcg ttggatcgtc ggaggcaagc tgtgttttgt gctcaatcat ctgggtctga
     3121 tcgtggattg gggggtcgcc acggctaacg tctacgacgg cagcgcctat cagcaggttg
     3181 tcgagcgcaa tgctgcccag atggtgctgt tacgcgatga aggctgtgtc aagaaagact
     3241 ggcatccgga aaacctcaag atttgccagc ggggccagtg gaatacccgc atgattgctg
     3301 aaacggtgct ctccaggttg accctggtgt gccatttcaa gaaggtgatg caccgggttt
     3361 gggcttattt ccagagccga ttggcttaca ccttggcgat gttcaacctg ctcgtccagt
     3421 gggacggcat ccagaccgat gaagatggct gcgtgcaatt ctctctcgct ccgttcagcc
     3481 tataaaacta gcaccaaagg ttaattattc tactattcaa tgttgtgcaa tctcttttta
     3541 ggtaaaataa aagtaaaccc tatccagttc atttaaggag aatgtgatga tgcaacaaag
     3601 aataggaaat atcgccgtcg cactggtatt gattctgctg ggcgtctggt tcctatctat
     3661 ccagttgttt cctggactga gagacttcgc ctacaaccgc tttacctggc cggtggcggt
     3721 gattggcgct ggggttctca tgggagttat tggattggtc acatgggttc ctggcttgct
     3781 gatcccagct tgtatcgtgg gtggcatagg cggcctgttg tactggcaaa atgctactgg
     3841 caactgggac agttgggcat atgcctggac gctgatccct ggttttgcgg gtgtcggcgt
     3901 catcctatct ggcttgatga gtcgcgaccg gcgtgcactg gtgggcggtg gatgggcgat
     3961 tttcaacagc ctggtcttgc tggcaatatt tggttcattt ctcggcggga tggcaatctc
     4021 aagatactgg ccggtattat tgattttgct gggtgttttg ctcctcgccc aggggttatt
     4081 ccggcggaga tgatgccctt gtttgtttga gatggcgaag caagcccact ttcgctttta
     4141 cgctgagctc aacgacctgc ttggcaaacc cgaggtagag gtccaagtcg ctttgtccca
     4201 gaatggggac caatcggtca agcacatgat tgagtcgctt ggggttccgc atactgaagt
     4261 aggacgggtg ctagtaaatg gcctcgcggt ggattttagc tatcttgtcc aggaaggcga
     4321 ccgggtcgag atttatccat tggcttgcca agagcctcca actccttcgg caattccccg
     4381 ctttatcctg gataaccatc tgggcaggct ggctgtctac ctgcgcatgc tgggttttga
     4441 tgcgatgtat cgcaatgact ttcaggatga tgaactcgcc agcctttgcg accgcgaaga
     4501 ccgtatcttg ctcacccgtg ataaacgcct gctgatgcga aatcaggtca cgcgtggtta
     4561 ctggctgcgc agtaagatac cccgtcgcca gcttgaagag gtggtgcgca ggtatcgctt
     4621 ggctggggcg attcaaccat ttcagcgctg catgcgctgc aatgagccgc tggtgccggt
     4681 acataaagat gttatccttc accgcctcga accgctaact cgccgctatt ttaatgagtt
     4741 ccatctatgt ccaacctgcg acaaggtgta ttggaagggc tcgcactacg agcgcatgcg
     4801 gcttctcatc gagcaagtta agattcacgc aattgaaggc aaagatactt tcccaacggc
     4861 ataactttta cgaggattaa gattcttgta atgtatttcc tgctctaggt aagagcttat
     4921 gggttatgat aagtgatcta tgagcagttc gcggatccta gttgtcgaag atgaacccag
     4981 tattgctgag gtgactagct tgtatctcaa gcgggctggt tttcaagttg tggtcgctac
     5041 agacggtgaa gaagccctgg atgagcttgg tagacagttc cctgacctga ttgttctgga
     5101 ccttatgctg cctggcatgg atggcatgga aatcacccgc catgtgcgtg ccatgagcga
     5161 tgtccccatc atcatgctga ccgctcgtcg ggaggaagcg gatcgaatcg ctgggctgga
     5221 gacgggggct gatgattatg tagtcaagcc tttcagcccg caagaattgg taagccgtgt
     5281 gcgagccgtg ctgcgccgca gccagggaca atcccaggtg agcaatgagc gcccattggt
     5341 cttcggtgat ctggtcatcg atccgcagac ccgcctggtt actgtacagg gggtagaagt
     5401 actgctgacc gtgaaggagt ttgatatgct ttggctgctg gcgcgccacc cgcgacaggt
     5461 gttctccaga cggcaactgc tcgagcgagt gtgggggctt tccgattaca ttgatccggg
     5521 cacggtcaca gttcacgtgc gccgattgcg ggaaaaaatt gaggcagacc catcgagccc
     5581 ggcacacatc ctcaccatgt ggggtgtcgg gtataaattt gaaccctgaa aaaaactgat
     5641 gagcaccggt agtctatcac gagtcaatct tcccgttcgc ttcattattg gggtggcgtt
     5701 aatagtagcc ctctcgctgg gaatctttaa tttactcatg cgacccgcgg tcaatgagat
     5761 tggcctgatg gcgggttttc tctccatcac tgccctggtc tctgctctgg caggctatgg
     5821 tttgtatcgt ctgggctgga tggaccgctc tccgaccatc ctttggtctt tgctgggtat
     5881 ttatgctctt tcgagtgtgc ttacctttct caacgtctgg ttgactgccc gcttgatgtt
     5941 tgccagtcag catgacctgc tcctcgccac cgtgctgctg atctttgctg ggggaattgc
     6001 catggcgctg ggttatttcc tctccagcac gcttaccgac cgcatccgcc tgatcgacca
     6061 ggcggctaag gcaattgccg ctggagattt ggatgtgcgt attcctgtcc gcgggcggga
     6121 tgagatggcc ttgctggcgc gcactttcaa ccagatggca gaacagctcc aggcagctgc
     6181 ccgtaagcaa caggagctag agaacttgcg ccgcgacctg attgcttggg ctgggcatga
     6241 cttgcagacg ccgctgacct cgatacaggc tgtcttagaa gccctggcgg atggggtggt
     6301 ggacgacgct gagaccattc aacgctacct aataactgcc caaagaaata tccaggctct
     6361 ctccgtctta gttgatgacc tctttcagat ggcgcaactg gacgcgggag ggctgccttt
     6421 ggatcgcgcc tataacagcc tgagcgacct gatctctgat acactggaga gcttctctga
     6481 actggcggct cgccagggtg tggatttgca gggcagcgta gacccgggag ttgacctggt
     6541 ttatatggat gcgcagcgta ttggacgggt gtttaacaac ctattgaaca atgccttgcg
     6601 atatacgccc cagggtggtc gtgtgcaggt gcaggcgcga cgggaagccg atgctatcct
     6661 ggtagaagtg atcgatacgg gacagggtgt ccagccggac gacctgccct tcatctttga
     6721 gcgtttttac cgcggcgaga agtcgcgcag ccgctctacg ggcggctcgg ggcttggtct
     6781 ggcaattgcc aggggcgtcg tggaagcgca tgggggacag attggtgtgg aaagcagccc
     6841 gcaaggcacg cgatttttct ttacgattcc gctaaaggac atcaggaggg ataacaatgt
     6901 caggcgttga ttttctttcg gagctgtacc ctcccgaccg attgctcacc cgcccggcgc
     6961 aattagctcc ctatgagtcg gacgcgctga cagctttgcg cgcccgcccc aaagcggtgg
     7021 tcctcccgga gacgcagggt gaggtcattg aaactgtgcg cctctgctac cgtaatagtg
     7081 tgcctttcgt agcgcgcggc agcggcacca gcctgtctgg aggttccacc cctgttgccg
     7141 aaggagttgt catcgccctc aaccgtttaa accgcatgat acgactagat cccgatcagc
     7201 gcctggcggt ggtggagccg ggaatgatca accaggacgt tagccgtgcc gccgcgccgt
     7261 acgggctgta ttatgcacca gacccctcca gccagccgat ctgtaccatt ggcggcaatc
     7321 tggcatttaa cgcgggcggg gcgcactgcc tgaaatacgg tatgaccagc aaccacgtgc
     7381 ttggcataaa agccgtgctg gcagatggcg aggtggtgca gcttggcgga gacagcctgg
     7441 aggggagcgg cccagacctg gtgggattgt ttgtcggttc ggaagggttg tttggcgtgg
     7501 ctctggaggt caccttgcgc ttgttgccta aacccgaagt ataccgaaca atcctggccg
     7561 cgtaccgctc cttacaggct gcaggccaaa ccgtatctca gatcgtggct tcgggtctgt
     7621 tgcctggtgc gatggagatc atggataact tggcaatcca ggctgccgag gccgcggtga
     7681 gcgctggcta cccgcttgac gctgcaagcc tattgattgt ggagctggac ggcgaggccg
     7741 cctcagtgtc tgctgattac ctcaaattaa tggagattat ccgcgcctcg gatccctacg
     7801 aagtccatga ggcgaaagat gatgccgagc ggttgcgcat ctggaaaggg cgcaaaagcg
     7861 ccttttctgc tgtgggccgg ctcagctcag attacctggt tcaagacggc gtggtgccgc
     7921 gcagccgcct gggagaggcc ttggcggaga tacaacgcct gagtgcccag tacaacttgc
     7981 gggtggcgaa cgtcttccat gccggcgatg gtaacctgca tccattgatc ttgtac
//
LOCUS       DBNHOH010000008         8365 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1538395, whole genome shotgun sequence.
ACCESSION   DBNHOH010000008 DBNHOH010000000
VERSION     DBNHOH010000008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8365)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8365)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8365
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1538395"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(264..944)
                     /locus_tag="AC16V7_00125"
     CDS             complement(264..944)
                     /locus_tag="AC16V7_00125"
                     /inference="COORDINATES: protein motif:HMM:NF022441.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HIR6255552.1"
                     /translation="MNKTYTETETRQISDFDQVLIQGLAYSAQLIMSQGEQEELTIEA
                     PPDYLDRLRSEVKDRKLTVRLRGGWLQELEDALATCLNRPHIVYRLKVRELSHLEVQC
                     AYSIHASSIETPHLHIKLSGTGDFKLDWLVAETLEVHHSGCGIMRISGKVEEQTVVLN
                     GVGSYIAPSLDSQRARVRMTGTGLARIQVSQALDATLRGVGSLEYSGHPGVSKRISGP
                     GQILHITN"
     gene            complement(1013..1759)
                     /locus_tag="AC16V7_00130"
     CDS             complement(1013..1759)
                     /locus_tag="AC16V7_00130"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6255553.1"
                     /translation="MNTVNWIQSYTLLLRWQGLKFKTVLPFVIVAQFFTGIGTVIGLG
                     YLMPDIDSQTAMFLVTGGPTLSLVTLGLVMVPQMVAQAKTGGMLDYMRSFPVPRMAYL
                     AADLTIWLLSTLPGVLLALVVGSLRYNFELQFSLLIVPAFMLVVLMATSVGYAIALLS
                     PKAELVGVLTNLIIFSLFLFSPVNFPVERLPHWLETVHRFLPVKYAADAVRGALVPGY
                     SDGLGLALAVLAGWCLVGLVINYMVATRRG"
     gene            complement(1774..2709)
                     /locus_tag="AC16V7_00135"
     CDS             complement(1774..2709)
                     /locus_tag="AC16V7_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015786143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6255554.1"
                     /translation="MALLEIEHVSKAYRGGVLANDDISLSVEAGEVFGLLGPNGAGKT
                     TLVHQIIGLLAPTSGTIRVAGINTVESPGFARQACSFQPQTQVPIDGMTPYQAIELAG
                     RLRGGRTDEVRARTRALIDSFELGAWSNKSGEALSGGVRRLVAFCMAAVVPGQITILD
                     EPTNDVDPLRRRLLWQQVRQLADQGSAVLLVTHNVLEAERSVDRLAIIDRGKVIGSGT
                     PGSMKNGDGDYLRLELLLEPGTSLPLLPNFLSHTVNGGRRLIGRVSAEDVTKAAMWAN
                     RMKADKLVEEYSLGPATLEDAYIRKIGRADALETI"
     gene            complement(2835..5489)
                     /locus_tag="AC16V7_00140"
     CDS             complement(2835..5489)
                     /locus_tag="AC16V7_00140"
                     /inference="COORDINATES: protein motif:HMM:NF037660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR6255555.1"
                     /translation="MSRSRLLQVLDAGLHRKLTLISAPAGYGKSTLLSEWASGCEWPL
                     GWVTLEAGDNDIERFLTYLMSAVQTAEESVPKLEDILGARFSLQPMSRDAMLAILVNQ
                     LSSTSERLVIVLDDYHLIENQDIHGFLNALLDNLPVNIHIVISTRTDPPLRLARLRAK
                     DQLNEITEKGLRFTPQEVETFFDVVMGLRLTDDEVAELEARTEGWVTGLQLVGLSLKD
                     REHPSELIHTLAGTHRYILDYLVEEVFSDLPPLLQTFLLRVSVLEHLSPELCDTVVGE
                     PDVPQQSKKILEFMDASNLFIVPLDSQRQWYRFHPLFADFLRDRLVAQHAEELPDLHR
                     RAAAWYASRGLLSEAIRHCFAGGDVDRAADLIQAEARDLLTRGEITTLLRWIDALPEE
                     VIAARPHLGLSRAWAMLMREPINFLDTIDQQIDQIAEGFGILSADLLSALAESEPESL
                     RRAGLSEFAMLLAFARRDNHDVDDTIKLFKAALEYMPESEHLLRGFTLAGLASTYARK
                     GAIKLAEDAFAQAAQHSLAANSIYGYVACTDWLATMQAEQGQLTRAAATYRQAIKLLT
                     SQAQYPLPLSGHVYVGLADVLLEQNDLQGAFENLQVGLQVGVQVRDVDALLNGYVIQA
                     RVLQMLNKGEEAQEAMQNAERQALETKSMSCVHDAQAHKARLALALGDVQVAQRWAAG
                     RGLGSVGDVHMEGPPNEIEQLTYARLLMAVGKASEALPILEALSGLQEQIGRTRALIE
                     SLTLQALCLRSLGRMDEAVRTLARALMLAAPEGFARVFIQEGPPLAALLRTVGSLGHS
                     PEYVKHLLEIFGEAPALQESVLDPLSERELEVLRLVADGLTNAEIAAQLVIAHSTAKT
                     HINRIYSKLGVSTRTQAVARARQLQILP"
     gene            6338..6820
                     /locus_tag="AC16V7_00145"
     CDS             6338..6820
                     /locus_tag="AC16V7_00145"
                     /inference="COORDINATES: protein motif:HMM:NF044704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HIR6255556.1"
                     /translation="MNFNEGDRVVHKKFGVGIVVSIEGMNLSGNGQSLYYRVDFSKTT
                     VWVPVSNQSEGLRLITPKSHLYRYRALLKSSPVSIDGDFHKRRSELEKRIDRGTFQGL
                     CEVIRDLNGRNAEKPLNYYEKTLLKRTREALISEWSITSGISRSETIDEINGCLEHRK
                     "
     gene            6830..7330
                     /locus_tag="AC16V7_00150"
     CDS             6830..7330
                     /locus_tag="AC16V7_00150"
                     /inference="COORDINATES: protein motif:HMM:NF044704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HIR6255557.1"
                     /translation="MSERGMSFQVGDRVIHWMYGLGEIIQLDEKVLSGHTGKYYVVQI
                     RDLTLWVPLSESGELCLRFPTPARDFKKLFHLLASPGESLSTERYLRKIQLTDLLKDG
                     TLESICRVVRDLVHFKRNNKLNENDKSTLERAKTFLLDEWSVVLSIPVQQAEIELTNL
                     LETSVV"
     gene            7426..7755
                     /locus_tag="AC16V7_00155"
     CDS             7426..7755
                     /locus_tag="AC16V7_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA recognition motif domain-containing protein"
                     /protein_id="HIR6255558.1"
                     /translation="METKLYVGNLAFETTEEDLHTLFTQAGTVASVDLIKDRASGRSK
                     GFAFVVMGNQSELEKAIQMFNSYTLDQRQLTVNIARPREERSQGGNGGNWQGERKHTG
                     RGGSRRY"
     gene            7915..>8365
                     /locus_tag="AC16V7_00160"
     CDS             7915..>8365
                     /locus_tag="AC16V7_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR6255559.1"
                     /translation="MNFDSFAFHPRINSGISAAGYSIPTPIQMRAIPPILEGRDVLGL
                     AQTGTGKTAAFVLPILQRLLNGPRGRLRVLIISPTRELAEQTHKAIGTLGQQTRLRSM
                     TIYGGVSVQPQIQRLRTGVEIVVACPGRLLDLMGQRVINLNSVETLVL"
BASE COUNT         1973 a         2293 c         2200 g         1899 t
ORIGIN      
        1 tgaatcaccc cgcttgcgaa gacgatacaa aaagccgcgt ggaaaaacta cgcgcaacct
       61 tggagcccaa attgaactcc aaacaagtca tggcggcgcg cactttggca accgagaaaa
      121 cgtttgatat gatcgtgaaa gaagccttgg aaacggcata aggatattgc aggcataaag
      181 ccttgcgttg tgccaggtag tatgctcagc tcaattccct tcgcttcgca ctgccagccc
      241 attggctggg gatttctttc tcactaattc gtgatatgca gtatctgtcc tggaccagaa
      301 attcgcttgc ttacgccggg atgcccgcta tattccaagc tacccacgcc gcgcagcgta
      361 gcgtccagcg cctggctgac ctggattctg gctaaacctg tgcctgtcat gcgcacgcgg
      421 gcgcgctggc tgtccaacga aggggcaatg tagctgccta caccgttcag cacaacagtt
      481 tgctcctcca cctttcctga gatgcgcatg atcccgcagc cggagtggtg cacttccaag
      541 gtttcagcca caagccagtc aagcttgaaa tctccagtcc cgctcaattt tatgtgaagg
      601 tgcggagtct ctatactgga tgcatgtatg ctgtaggcac actgcacttc caggtggctg
      661 agctcgcgca ccttcaaccg gtagacgatg tgcggcctgt tcaggcaggt cgcaagagca
      721 tcttctaatt cttgcaacca gccgccccgc aagcgcacgg tgagctttct atctttcacc
      781 tcgctgcgca acctatccag gtaatctggg ggtgcttcga tggtcagctc ttcctgctca
      841 ccctgagaca tgatgagttg cgcgctgtaa gccaaacctt ggatcaatac ctggtcaaaa
      901 tcgctgatct gccgggtctc tgtttctgtg tacgttttgt tcatgatagt tatcgttgtt
      961 agaaacagtt atttatagta attccaattg tttgcaccgg gcgtactggc tatcaacccc
     1021 ggcgggtagc taccatataa tttataacca gccctaccag acaccaacca gccagcacag
     1081 ccagcgccag acccaatcca tccgaataac ccggcaccag cgcgccgcgc accgcatcgg
     1141 cagcatactt caccggcaag aagcgatgga ctgtctcaag ccagtggggc aggcgctcga
     1201 cggggaagtt gaccggcgag aacaggaaca ggctgaagat aattaagtta gtcagcacgc
     1261 ccaccaattc cgctttcggc gagagcaagg cgatggcgta gcccactgaa gtcgccatca
     1321 gcaccaccag catgaaagcc ggcacgatca acaggctaaa ttgcagctcg aaattgtagc
     1381 gcagcgagcc tacaacaagc gccaggagca cgccaggcag ggtggacagc aaccatatgg
     1441 tcaaatctgc cgccaggtaa gccatgcgtg gcacaggaaa ggagcgcata tagtccagca
     1501 tcccaccggt tttcgcctgt gctaccatct gcggaaccat cactaatccc agtgtgacca
     1561 gcgagagtgt tggtccgcca gtcaccaaga acatggctgt ttgggagtcg atgtctggca
     1621 tcagatagcc taggccgatg accgtaccga taccggtgaa gaattgagcg acaattacaa
     1681 acggcaggac agtcttgaac ttcagcccct gccatctgag caatagggtg tagctttgga
     1741 tccaattaac tgtgttcatt tgatatatct ccttcagata gtctccaagg cgtctgcgcg
     1801 cccgatttta cgaatgtatg cgtcctccag ggttgccggc ccaagggagt attcctccac
     1861 gagcttgtcg gctttcatgc ggtttgccca catggctgcc ttagttacat cctcggcgct
     1921 aactcttccg ataagacgcc gcccaccgtt gaccgtgtgc gataggaagt ttggcagcag
     1981 gggcagcgaa gtcccgggct caagcagcag ctccaggcgc aagtaatccc cgtcaccatt
     2041 tttcatggaa cccggcgttc cagaaccgat gactttgccg cggtcgatga ttgccaaacg
     2101 atcaaccgag cgctccgcct ccagcacatt atgggtaacc agcagcaccg cgctaccctg
     2161 atctgctagc tggcgcacct gctgccacag caagcgccgt cgcagcggat caacatcgtt
     2221 agtaggttcg tccaagatgg tgatctgacc aggaacgacc gctgccatac agaaagccac
     2281 cagccgtcgc acgccccctg agagagcctc accactttta ttcgaccacg cgccgagctc
     2341 aaagctgtcg atcagcgctc gggtcctggc gcgcacctcg tccgtacgac cgccgcgcag
     2401 acgtccggct agctcgatag cctggtaagg tgtcatacca tcgatgggaa cctgcgtttg
     2461 gggctgaaac gagcatgcct ggcgggcaaa accaggggac tcgaccgtgt tgatgccagc
     2521 cacccggatc gtgcctgagg tgggtgccaa caaaccgatg atctggtgta ccagggtagt
     2581 cttgccggcg ccgttgggtc ccagcaggcc gaacacctca cccgcttcaa ccgacaggct
     2641 gatgtcgtca tttgccagca ccccgccgcg gtaggcttta cttacgtgtt caatctctag
     2701 caatgccatc ttctttcctc caaaacaaat aaactcatgg tgtggataat ctttcgccat
     2761 gagtttacaa aaaaaatgga ggaaaagcgt ccacccgggg gtggattggg gggtggaatg
     2821 aaaaaagggg aggattaagg caaaatctgg agttggcggg cgcgggcgac cgcctgggtg
     2881 cgggtgctga cgccgagttt gctgtaaata cggttgatat gggtttttgc cgtgctgtgg
     2941 gcgatgacca gttgagccgc gatctcggcg ttggtcagcc cgtctgctac caagcgcagc
     3001 acctccagct cgcgctcgct gagtggatca agcacggatt cctgcaatgc gggggcttcg
     3061 ccgaagatct caagaaggtg cttgacgtat tccggcgagt ggcccagcga cccaaccgtg
     3121 cgcagcaacg ccgccaacgg aggcccttcc tggataaaga cgcgagcaaa accttccggc
     3181 gcggcgagca tcagggcacg cgccagcgtg cgcaccgcct catccatacg tcccaaggaa
     3241 cgcaggcata gcgcctggag cgtcagactc tcgatcaggg cgcgcgtgcg cccgatttgt
     3301 tcttgcaggc cactcaaagc ctccaagatg ggaagcgcct cagatgcctt ccccactgcc
     3361 atcagcaagc gggcataggt cagctgttcg atctcatttg gaggaccctc catatgaacg
     3421 tctccaacgc ttcccaagcc acgccccgca gcccagcgct gggctacctg gacatctcca
     3481 agagccaggg caaggcgcgc cttatgtgct tgcgcatcat gcacgcagct catgcttttg
     3541 gtctctaggg cttgtcgctc agcattttgc atggcttctt gcgcttcttc ccccttgttg
     3601 agcatttgta aaacgcgtgc ctggattaca tagccattca acagggcatc cacatccctg
     3661 acctgaaccc caacctgcag ccccacttgc agattttcga atgccccttg gagatcattc
     3721 tgttccagca gcacatcggc cagtccgacg tagacatgcc ccgaaagagg tagcgggtac
     3781 tgggcctgac tggtgagcaa cttaatcgcc tggcgataag tggcggcggc gcgcgtgagc
     3841 tgaccctgct cggcttgcat ggttgccagc cagtcggtgc aggccacata gccatagatg
     3901 ctgttggctg ccaggctatg ctgtgctgcc tgtgcaaacg catcctcagc cagtttgatc
     3961 gcgcctttcc tggcataggt cgacgccaac ccggctagag taaaaccgcg gagcagatgt
     4021 tcgctttctg gcatgtattc aagcgcggct ttgaacagct tgatcgtatc atcaacgtca
     4081 tggttgtcac gccgcgcaaa tgccagaagc atagcaaatt cactcagccc ggctcgccga
     4141 agcgattcgg gctcgctctc agccagggca ctgagcagat ctgcagacag gatgccaaaa
     4201 ccttcagcga tctggtcgat ttgctggtcg atcgtatcca ggaagttgat cggctcacgc
     4261 atgagcatcg cccaggcccg cgacagccct aaatgaggac gggcagcaat gacttcctcc
     4321 ggcaacgcat caatccagcg cagcagggtc gtgatctccc ctcgggtaag caggtctctg
     4381 gcctctgcct ggatcaagtc ggccgcgcga tcaacatccc cacctgcaaa acagtgtcgg
     4441 atcgcttctg agagcaagcc gcgactggca taccaggcag ctgcccggcg gtgcagatcg
     4501 ggcagctctt ccgcatgctg agccaccaag cggtcgcgca ggaaatcagc aaagagcgga
     4561 tgaaatcggt accactggcg ctggctgtca agcggcacga tgaacaggtt ggaggcgtcc
     4621 ataaattcca aaatcttctt tgactgctgc ggcacgtccg gttcccctac cacggtgtca
     4681 cacagctcag gcgacagatg ctcaaggacc gaaacacgca gcaggaatgt ctgcagcagg
     4741 ggaggaaggt ctgagaacac ctcctccacc aggtaatcca agatgtagcg atgagtgcct
     4801 gccagtgtat ggatcagctc gctgggatgc tcgcgatcct tcaaagaaag acccacgagc
     4861 tgcagcccgg tgacccagcc ttcggtgcgc gcttccagtt cggcaacctc atcatcagta
     4921 agcctcagcc ccatcaccac atcgaaaaag gtctccacct cctgaggcgt gaagcgcagc
     4981 cccttttcgg taatctcatt cagttgatcc ttggcgcgta aacgagccag ccgcaaaggc
     5041 gggtccgttc gtgtggagat cacgatgtgg atattcacgg gcaggttgtc gagcaaggcg
     5101 ttaagaaatc cgtggatgtc ttgattttcg atgagatggt aatcatccag cacgatcacc
     5161 agccgttctg aagtggagga taactggttt accagtatcg ccagcatcgc atcgcgcgac
     5221 atcggttgga gtgagaagcg agctcccagg atgtcctcca gtttaggcac gctctcttcg
     5281 gcggtctgta cagcagacat caggtaggta agaaagcgct cgatgtcgtt atcgcctgct
     5341 tcaagcgtga cccagcccag cggccattca cagcccgaag cccactcgct gagcagcgtc
     5401 gacttgccat acccagctgg tgccgagatg agggttagct tgcggtgcaa acctgcatcg
     5461 agaacctgga gcaagcggct gcgcgacacg agctgcagcc tagaaggcgg gatctgcacc
     5521 ttcgggagca ggatgggaag gggtgtagga atatccatga gcaggtatag cggtggccga
     5581 atataatggg tttgctggtt gaacgattat agcactacct gctcagctat actgcctctt
     5641 gctcattgat gaaattattg ccttcaaata gcaaaattgc acctacctat ttcattccct
     5701 gacccagcct cctcccaacg tctttacacc cgaccttccg ttgaggttca ccagatgata
     5761 ttgctgtctg cgggctactt tgtagtttag cagttcgctc caggcgtgca caaggggttg
     5821 gtcatcttac tgctttgttc tctatcatca cggccttcaa ttaatccacg caaatttgag
     5881 cgaaaattct ggaacttaac aggtaatttt tacgatttat cctacaccaa ctcacaacct
     5941 ctagcaccct gccgaggcta ccccttgaaa gactcctgaa ccgtcgaatt gtacaggtct
     6001 acagggcagg atgggcatac cccccaaaaa taccccaaac actcttttta cgggattgcg
     6061 tatgcccctc agggcatatg caattgccct ggagtcacaa tttgcccagg tcagtaatat
     6121 gatataattt atataatctt acttgggata tgtggaatcg ccgctagtcc tcgtgcagga
     6181 taggcggctg tttttttcgc tacttctgtc gactagccga ttgaatattt aatcccgaga
     6241 tagcaattta gcagattgct tgggggcgag atgatccatt ggaggcctat tatttttacg
     6301 catctctgtt tcggtttatc agcctgtgga tacaaaaatg aacttcaatg aaggcgatcg
     6361 tgttgttcac aagaaatttg gagtaggaat tgttgtatca atcgaaggca tgaatttatc
     6421 cggcaacgga caaagcctct attatcgtgt ggatttttcc aaaaccaccg tttgggtgcc
     6481 agtaagtaat cagtcagaag ggttgcggct tatcacacca aagagtcatc tctatcggta
     6541 ccgcgcctta ctaaaaagct caccagtctc aatagacggc gatttccata aacggcgcag
     6601 cgaattggaa aaacggattg atagaggtac cttccagggt ttatgtgagg ttattcgaga
     6661 cctcaatgga cgcaatgctg agaaaccact gaactactat gagaaaacac ttcttaagcg
     6721 aaccagggaa gctctgattt cagaatggtc tattacgagt gggatctctc gctccgagac
     6781 aatcgatgaa attaatgggt gcttggagca tagaaagtga gttcagtcca tgagtgaacg
     6841 gggtatgtct ttccaggttg gagaccgggt gatccattgg atgtacggtc tcggagaaat
     6901 tatccagctg gatgaaaaag tgttgtctgg acacactggc aaatattatg ttgtgcagat
     6961 tcgcgatcta acactttggg ttccgctaag cgaaagtggt gagctctgcc tgcgtttccc
     7021 aaccccagca agagatttta aaaaactctt tcatcttcta gccagccccg gagaatcgct
     7081 atcgactgaa cgttatctac ggaaaataca gttgacggat cttttgaagg acggaacgct
     7141 cgaatcgatt tgccgggtgg ttcgcgatct ggtgcatttt aaacggaaca ataaattaaa
     7201 tgaaaatgac aaatctaccc tggaacgcgc caagactttc ctgttggatg aatggagcgt
     7261 ggttttatcg attccggtcc aacaagctga gatcgagttg acgaacttat tggagacaag
     7321 tgtggtataa tttattatct tactactggg tagagttacc gtcggcccct tctgtgaggc
     7381 tggcggttct tttttttaat acatctcaaa ggagatacac ggattatgga aaccaagctt
     7441 tatgtcggta atcttgcttt tgaaactacc gaagaagatt tgcacacgtt gtttactcag
     7501 gctggtacag ttgcctctgt agacctgata aaggatcgtg caagcggcag gtcgaagggt
     7561 tttgcctttg tagtgatggg caatcaaagt gaattggaaa aagccattca aatgttcaac
     7621 agttacaccc tcgaccaacg ccaattaact gtcaatatcg ctcgtccgcg cgaggagcgc
     7681 agccagggcg gaaatggcgg caactggcaa ggagagcgta agcacaccgg tcgcggcggt
     7741 tcccgtcgat attaagcctt agcatgtaat gttgatagaa gggctccaag ccggtttgcg
     7801 cgcctggagc ccaaacccac tatataaacg actttcagga cactttgcag ttcgagcagg
     7861 acacctggac gtatggtctc gaacgagttc ctatttgtcg ttaaaggatc tgctgtgaat
     7921 tttgactcat ttgcttttca tccccggatt aattccggaa tctcagccgc tggctactcc
     7981 atccctaccc cgattcagat gcgtgccatt ccacccatct tggaagggcg tgatgtgctg
     8041 ggcttggccc aaaccggcac cgggaaaacg gctgcttttg tattacccat cctccaacgc
     8101 ctcttaaacg ggccacgtgg caggttgcgt gttctcatca tatctcccac gcgcgagctg
     8161 gccgaacaga cccataaagc cattggaacg ctcggtcagc agactcgtct ccgaagtatg
     8221 accatatatg gaggcgtcag cgtccaaccg caaatccaga ggttgcgtac tggagttgaa
     8281 atcgttgtgg cctgcccagg ccgcttgctg gatctgatgg ggcagcgagt aatcaacctg
     8341 aattcggttg aaaccctggt gttgg
//
LOCUS       DBNHOH010000009         1951 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_705281, whole genome shotgun sequence.
ACCESSION   DBNHOH010000009 DBNHOH010000000
VERSION     DBNHOH010000009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_705281"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="AC16V7_00165"
     CDS             complement(<1..209)
                     /locus_tag="AC16V7_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255560.1"
                     /translation="MPCYIDDHQRLEATLRYNIQSNITAIFILIAGVGWIWLSKAFPD
                     DLSVDGKIAPRAGFLAPDFSLLDRD"
     gene            461..1321
                     /locus_tag="AC16V7_00170"
     CDS             461..1321
                     /locus_tag="AC16V7_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012314.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR6255561.1"
                     /translation="MKISVCLVFVSLLLLTACRGPETLEPVVTSQVSITPLGETGQLL
                     TPSPVDQKPLGGAYPEPEVTMPSQSLQSTSVATENEAYPPPGSTSVAGGSDAYPPPGT
                     LATTTGQELGNFNQPYPAPETDIPLGSTGTSQSQGISGQTAVSTPTTTLIPTSNPTIT
                     SISTATPTPTAPMGLVRTELRASDPSEFSIVSGELQLVMFFAHWSPLSKSMAPVMNAL
                     EEKYQDRITFFYLDIDDPANNFYKYLLGDRLPPVFFLIDPQGIVLNEWQGFVMPEEFE
                     AAFASVVITP"
     gene            1484..1807
                     /locus_tag="AC16V7_00175"
     CDS             1484..1807
                     /locus_tag="AC16V7_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255562.1"
                     /translation="MRNKLKYVWVNLFVFLLVALMAGCGRATQPEATNPVETPTASQT
                     VVIQPTPSATEASAPTQTPTTEIIIESPTAPPPTPRPGLEASEPSSFVQASGQYQLVE
                     FFAFW"
BASE COUNT          535 a          470 c          441 g          505 t
ORIGIN      
        1 ctgtctcgat ccagaaggct aaaatccggc gccagaaagc ccgcacgcgg cgcaatcttg
       61 ccatcgaccg ataaatcatc agggaatgct ttactcagcc aaatccagcc tactcctgcg
      121 atcagaatga atatagcggt tatattcgat tggatgttat accgcaatgt tgcctccaaa
      181 cgctgatggt catcaatata acatggcata aagatctcat caatggcgat gatctttgta
      241 agaccgtttg gacaccggtt gtggttatat ctcgtgcggc aatttcacgc cgtgcaggta
      301 atggagactc cccagcctgc ttgggtaatc acgttgtata tctttgagat aacatgttgg
      361 aatgtatacg gatcttgatc caattctgag gttactgggc ttgacatatt attgatcaat
      421 gatatgcttt ggtcagttag tttatgggtc tggaatttat atgaaaatct ctgtatgtct
      481 tgtgtttgta agcttgcttt tgctgacggc atgccgtgga ccggaaacgc ttgaaccagt
      541 ggtcacttct caagtgagca taactccact cggtgagaca ggtcaactac ttaccccaag
      601 ccctgttgat caaaagcctc taggcggagc ctatccggag cccgaagtta ccatgccttc
      661 gcaatcgctg caaagcacct ctgttgctac cgagaatgag gcttacccac ctccaggaag
      721 tacctctgtt gctgggggaa gtgatgctta cccgccccca ggcactttag caacaaccac
      781 tggacaagaa ttgggcaatt ttaatcaacc ttatcctgct cctgagacag atattccatt
      841 gggtagcaca ggtacaagtc agtcacaggg aattagtgga cagaccgcgg tgtcgacacc
      901 cacaaccacg ctaattccca catcaaaccc gacaatcaca tcaatatcca cagcgacacc
      961 aacccccaca gcgccgatgg ggctagtgcg cactgaatta agagcctcag accctagcga
     1021 gtttagcatc gtttctgggg agcttcaact agtaatgttt tttgcacatt ggtcaccgct
     1081 tagtaaaagc atggctcccg taatgaacgc tctggaagag aagtatcaag atcgaatcac
     1141 ttttttttat ctggatatcg acgatccagc caataacttt tacaaatatc tacttgggga
     1201 tcgtttaccg cctgtctttt tcctgataga ccctcagggg attgtcttaa acgaatggca
     1261 agggtttgtg atgccagagg aatttgaagc tgcttttgcc tcggtggtta ttacaccctg
     1321 atacccaaaa ttgtaatatt tatgtaatca ttgaataatt gacaatcaag ggggaaatgg
     1381 taagattatt ctcctagatg ttatccaaaa gaaaggggcg catgcaggtg gaagattaac
     1441 atcgctgcgt aatatttgga aaaacacacg aggataattg gcattgagaa ataaactaaa
     1501 gtatgtatgg gtaaaccttt ttgtcttctt gctggtagcg ctcatggcag gctgcggacg
     1561 agcgacacaa cctgaagcga cgaaccctgt tgaaacccca acggcttcac aaactgtggt
     1621 tattcaaccg actccttctg cgactgaggc aagtgcaccg acgcaaacac caaccacaga
     1681 aataataatt gaaagtccca cggcaccacc gcccacacca cgtcctgggc tagaagcgtc
     1741 tgagcctagc tcattcgtgc aggcctcagg tcagtatcaa ctggttgagt tttttgcctt
     1801 ctggtgaacg acctgccagc gaatggcgcc catcgtgcat gggttggaag ccaaatattc
     1861 taaccgcatc aagtttattt atcttgatat agacgaccca gctaatcaac aagccatgaa
     1921 ggatttagga tatatcggga gaccacattt c
//
LOCUS       DBNHOH010000010         2016 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_513186, whole genome shotgun sequence.
ACCESSION   DBNHOH010000010 DBNHOH010000000
VERSION     DBNHOH010000010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_513186"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /gene="fdhD"
                     /locus_tag="AC16V7_00180"
     CDS             <1..707
                     /gene="fdhD"
                     /locus_tag="AC16V7_00180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HIR6255563.1"
                     /translation="NGEAWLTFMCTPVDLEAMAVGFLFNEGLIKSADEVADVRLCDTG
                     DNLDVWLHRSVDKPDKWSRTSGCTGGMTSVESNITSPAITNGGVLYADRVGQLIAQLF
                     ESQQLYRASGGVHTSALSDGQRIWIIAEDIGRHNTLDKIAGHYLLEGLQPQKRIILTT
                     GRISSEMLQKSARLNSSVVISRTSPSSLSIDLAQKWGITLIGYARRNKFNIYTHPERI
                     VFAPEGKKAKEEPIFV"
     gene            745..>2016
                     /locus_tag="AC16V7_00185"
     CDS             745..>2016
                     /locus_tag="AC16V7_00185"
                     /inference="COORDINATES: protein motif:HMM:NF013662.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HIR6255564.1"
                     /translation="MTNHHIRFKPPDPQIAALVMAHAGKPEAVLEVLKGVQDLYGGLS
                     QENIQDVALSLNIPAERAYGVASFYSMLSLEPVEGKIIRVCDGPVCWLKRASEVQIAL
                     ESEAGRAGYTIERSSCLGLCDRAPAALVVQEQAGPVNPGDARKLVQGERGVSIDYSHP
                     RRGEVRVMMAQAGMVDPDSLQSALSHGAYEGLKKALSLTPEQVVAVVENSGLTGRGGA
                     GFPVGRKWRFVAQSGRMPRYVICNADESEPLIFKDRVLIDTNPHQILEGMAISGYATG
                     SHEAFIYIRGEYAAQAARLERAISQAEEANLLGESILDSGFSFHIHVHMGAGAYICGE
                     ETALIESLEGKRGEPRARPPYPPSYGYMGQPTLVNNVESYAAVPAILQNGAQWYRSIG
                     NNNGGTSQPGITPGTNLYMILGHINQPGLFEA"
BASE COUNT          504 a          504 c          536 g          472 t
ORIGIN      
        1 taaatgggga agcctggctt acattcatgt gtaccccggt agatttagag gcaatggcgg
       61 tcggattctt gttcaatgaa ggattgatca aatccgctga cgaggttgcc gacgtccgtc
      121 tttgtgatac tggggataac ttagacgtct ggctccatcg ttccgtggat aagcctgaca
      181 aatggagtcg cacatccggt tgcaccggtg ggatgacttc ggttgagagc aatatcacat
      241 ctccagccat taccaacggg ggagtccttt acgcggatcg agtggggcaa ctgatcgccc
      301 aattgttcga atcgcagcag ttgtaccgcg cttctggtgg agtacacact tccgccctca
      361 gcgatgggca gcgcatctgg ataatcgctg aagatatcgg tcggcacaat actctcgaca
      421 agatcgctgg tcattatctt ttggaaggtc tgcaacccca gaaacgaatc atccttacca
      481 ccggtcgcat cagttcagag atgctgcaaa agtccgctcg attaaattct tctgttgtga
      541 tttcccgcac atcacctagt tccttatcca tcgacctagc ccaaaaatgg ggaataacgt
      601 tgatcggata cgcccggaga aataaattca atatttatac acatccggaa cgaattgtgt
      661 tcgctccgga aggcaaaaaa gccaaagaag agcccatttt cgtatgaaaa tgggctcttt
      721 tatcataata agcgagaggg agcaatgacc aatcatcaca tccgctttaa accacctgat
      781 ccgcaaatcg ctgccctagt catggctcac gctggaaaac ccgaagctgt cttggaggta
      841 ttgaaaggtg ttcaagatct atatggcggg ttgtcccaag agaacattca agatgtcgcc
      901 ctcagcctaa atattccagc cgagagagct tatggtgttg catcttttta ttccatgctc
      961 tcattagagc cagtcgaagg aaagattatc cgtgtatgtg acggtccggt ttgctggcta
     1021 aaacgagcat ccgaggttca gatagctctc gagagcgagg ctggaagggc aggctacacg
     1081 atcgagcgca gcagttgcct ggggctatgt gaccgcgctc ctgctgcttt ggtagtgcaa
     1141 gagcaggcag gaccagttaa tccaggtgat gcgcgcaaac tggtgcaagg cgagcgaggt
     1201 gtgagtatag actatagcca tcctcggcgt ggagaagtac gcgtgatgat ggcacaggct
     1261 ggcatggttg acccagattc gctccaatct gccctaagcc atggtgcata tgagggcctg
     1321 aagaaggctt tgagtctcac tcctgagcaa gtagtagctg tagttgaaaa ttctggcctg
     1381 accggacgtg gaggggctgg cttccctgtt ggacgtaaat ggcgcttcgt cgcccaatca
     1441 ggtcggatgc ctagatacgt catatgcaac gctgacgaat ctgagcctct gatcttcaaa
     1501 gatagggttt tgatcgatac gaacccacac cagatcttgg agggcatggc aatctcaggc
     1561 tacgccacag ggtcgcacga agcgttcatc tatattcgtg gcgagtatgc agctcaagct
     1621 gctcgcctgg aacgcgccat cagccaggcc gaagaagcta atctgcttgg ggaaagcatc
     1681 ctggacagcg ggttctcatt tcacatccat gttcacatgg gggctggagc gtatatttgc
     1741 ggcgaagaga cagctctcat cgaatcgctg gaaggcaagc ggggtgagcc acgcgctcgc
     1801 ccaccctatc caccttctta tggctacatg gggcagccca cccttgtaaa taacgtggag
     1861 tcatatgctg ctgtgcctgc catactacaa aatggcgcac aatggtatcg ctcgattggt
     1921 aacaataacg gcggcacatc ccagcctggg ataaccccag gcacaaactt gtacatgatc
     1981 ttgggccaca ttaatcaacc agggctattc gaagcc
//
LOCUS       DBNHOH010000011         2299 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1410457, whole genome shotgun sequence.
ACCESSION   DBNHOH010000011 DBNHOH010000000
VERSION     DBNHOH010000011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1410457"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="AC16V7_00190"
     CDS             <1..649
                     /locus_tag="AC16V7_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6255565.1"
                     /translation="DSLAKSGLRVVDRLHRRGILGPRTIVAHAVHVDAREAGLLADSG
                     TWVTHQPRSNMNNGVGVAEVESLMRLGVPVCMGTDGFSHTMWVEWKTTYLLHKVWHRD
                     PRRMNGMDVVAMGVYNNAALAGGFFPQAALGVIKPGAHADLIFVDYQPHTPLTSDNVP
                     WHIIFGFHESMVTTTIVAGQVLMRDRQLLTLDEEAIAARALEQAPGVWERYNAQF"
     gene            663..1322
                     /gene="npdG"
                     /locus_tag="AC16V7_00195"
     CDS             663..1322
                     /gene="npdG"
                     /locus_tag="AC16V7_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007228558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0006740 - NADPH regeneration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HIR6255566.1"
                     /translation="MTETLPIIAILGGTGKEGPGLALRWGGAGYPIIIGSRQTEKAQA
                     TADELNAMLGGNSVQGMENGLAARNADICVLTVMYTAHQSAIDSLIDDLQGKILVDAT
                     ARVDFRDPRPPDPPSAARIAQDKLGTGVRVVAAFQNVPAHALKKNLGQPLDSDVLVCA
                     DDVEAANEVIKLAEAGGMRAYYAGGLDNAIVVEGLTSIMISLNKHHGVKTASIGIRGI
                     R"
     gene            1359..2126
                     /gene="cofE"
                     /locus_tag="AC16V7_00200"
     CDS             1359..2126
                     /gene="cofE"
                     /locus_tag="AC16V7_00200"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HIR6255567.1"
                     /translation="MPLTLTPLPGIPLIQPGDDLAEIIMQSLGREEIVLQDGDILVLA
                     QKIVSKSQGRRVNLATINPSQTALELASQTDKDPRFLELVLRESSEVLRTRPGSIIVE
                     HRLGFVCANAGIDHSNVAGESIQDEEWVLLLPEEPDNAAEAIRRCLEAASGVRLGVLI
                     IDSHGRAWRLGTVGVSIGLSGLPALVDLRGQEDLFGYKLRITQVGAADELAAAASLVM
                     GQADEGIPVVHVRGFPYPLREASLRELLRPKSQDLFR"
BASE COUNT          492 a          601 c          688 g          518 t
ORIGIN      
        1 cgacagcctc gccaaatcag gtttgcgtgt ggtcgaccgt ttgcaccggc gaggtattct
       61 cggtccgcgc accatcgtgg cccacgcagt gcacgtggac gctcgcgagg ccggtttgct
      121 ggcagatagc ggcacatggg tcacccacca gccccgctcc aacatgaata acggcgtggg
      181 agtggcggaa gtggagtctt tgatgaggct cggcgtaccg gtgtgcatgg gtacggacgg
      241 cttctcccat accatgtggg tggagtggaa aacaacctac ctgctgcaca aggtgtggca
      301 ccgcgatccg cgtcgcatga atgggatgga cgtggtcgcc atgggcgtgt ataacaacgc
      361 cgctttggca ggaggtttct tcccgcaggc ggctttgggc gtgatcaagc ccggtgctca
      421 tgccgacctg atttttgttg attaccagcc gcatacgccg ctcacctcgg acaacgttcc
      481 ctggcatatt atctttggct ttcacgagag catggtgacc accactatcg tggcaggtca
      541 ggttttgatg cgggatcgcc agttgctcac actggatgag gaagctatcg ccgcccgtgc
      601 ccttgagcaa gctcctgggg tgtgggaacg ctataacgcg cagttttgaa acagggaaat
      661 ttatgacgga aacattacca ataatcgcta tcttaggtgg gactgggaaa gaagggccag
      721 ggctggcact tcgatggggc ggcgctggtt accccatcat catcggctct cggcaaacgg
      781 aaaaagcaca ggcgactgct gatgaattaa atgccatgct tggcgggaac tccgttcagg
      841 gcatggaaaa cggtcttgca gcgcgtaatg ctgatatctg tgtgctgacc gtgatgtaca
      901 ctgcccacca gtcagccatt gacagcttga tagacgatct acagggcaag atactggttg
      961 atgctactgc acgcgtggat tttcgcgatc ctcgcccgcc tgacccgccc tcggctgcgc
     1021 gcattgccca agataagctt gggacgggtg tcagggtggt ggctgccttc cagaatgtgc
     1081 cggctcacgc cctgaaaaaa aacctggggc agccattgga ttccgatgtc ctggtgtgcg
     1141 ctgatgacgt agaggctgcc aatgaggtga tcaaactggc tgaagctggc ggcatgcgcg
     1201 cctattacgc cggcgggctt gataacgcca ttgttgtcga gggcttaacc tcgattatga
     1261 tcagcctgaa caagcaccat ggcgttaaga ctgccagtat tggaataaga gggatccgat
     1321 aaaaactaga agggttctac tgttgcttat cttctgatat gcccctaacg ctaacacccc
     1381 ttcccggtat ccccctcatc caacctgggg atgacttggc tgagatcatc atgcaatccc
     1441 tggggcggga agagattgtg ctgcaggacg gcgatatact cgtcctggca caaaagattg
     1501 tctccaagag ccagggacgc agggtaaatc tggcgaccat caatccatcg caaacagctc
     1561 ttgagctggc ttcacaaaca gataaggacc cccgtttttt ggagttggtt ctgcgggaga
     1621 gcagcgaagt gcttcgtacg cgccccggat caattattgt cgaacaccgc ctgggttttg
     1681 tctgcgccaa tgcaggtatc gatcactcca atgtagccgg ggagagcatc caggatgagg
     1741 agtgggtctt gctattgcct gaggaaccag acaacgccgc tgaggcaatc cgccgctgct
     1801 tggaagctgc cagcggggtg cgattagggg ttttgatcat cgattcacat gggcgcgcct
     1861 ggcgtttggg gacggtgggg gtttcgattg gcctctcggg tctaccagct ctggtcgacc
     1921 tgcgcggcca ggaggatttg ttcggttata agctgcgcat cacccaagtt ggcgctgctg
     1981 acgagctggc tgccgcagct tccctggtca tggggcaagc tgatgaaggc ataccggtgg
     2041 ttcatgtgcg tggttttcca tacccgctgc gcgaggcctc gctgcgggaa ttattgcgac
     2101 caaagagtca agatttgttc cgttagaaga aggaggttgt ctaaatgcct gcccaagcga
     2161 tcaatctaga gaaatttaat ttcgatgccc ggcccgatac catcgacttt cgagacttga
     2221 tgtacacgcc cacactggta gaggtgccgc tgcgcattcc gctcgagaaa tataaagatg
     2281 ccagagtgcc gatcttgaa
//
LOCUS       DBNHOH010000012         3354 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1730898, whole genome shotgun sequence.
ACCESSION   DBNHOH010000012 DBNHOH010000000
VERSION     DBNHOH010000012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1730898"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1234
                     /locus_tag="AC16V7_00205"
     CDS             170..1234
                     /locus_tag="AC16V7_00205"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6255568.1"
                     /translation="MQLPGLIDPHVHMREPGATHKEDWGSGTSAALAGGFTIVLAMPN
                     TQPPVTDRDSFSMALKAARLKALCDYAQYLGAGPDNVEVLPRMALRSAGLKMYLDQTY
                     GPLRLDDMALWMAHFAQWPRQMPIVAHAEGSTLAAVILLALLYDRPLHLAHVSRRDEI
                     LLIRAAKERGIRITCEVAPHHLFLTHDDLPGGRGEVRPRLATAADRDALWANMDVIDC
                     FATDHAPHTQAEKDSPNPPPGFPGLETALPLLLTAVNEGRLNTDDLLTRMVNNPRRIF
                     NLPEQPDTWIEVDTDDVWEIRAADTFTRCAWTPFEGWKVRGRVRKVTLRGREVFRDGK
                     ILAPAGSGKHIRDENRSLAR"
     gene            1244..2236
                     /gene="pyrB"
                     /locus_tag="AC16V7_00210"
     CDS             1244..2236
                     /gene="pyrB"
                     /locus_tag="AC16V7_00210"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008085852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HIR6255569.1"
                     /translation="MTTFSPPSRVQNPFLPFGDQRKAPFYGKDIISVKQFSRPDLDYI
                     FGVAHEMREMVERIGTFDLLKGKILANLFYEPSTRTSSSFTSAMERLGGSVIPINEVR
                     YSSVSKGESLPDTVRTLECYADVIVLRHPEVGASALAAQYARKPIINAGDGIGEHPTQ
                     ALLDLFTIIEELGQADSLTVTMLGDLKYGRTVHSLARLLSLYNVSLHYVSPDILRMPT
                     EIIAELKEKGIPQVEHSTLDEVVPKTDVLYVTRVQKERFADQAEYESVKGAYVITPDI
                     MSKAKERMIVMHPLPRVGEISIEFDSDPRAAYFRQMEYGLYVRMALLAMVFGKA"
     gene            2325..>3354
                     /gene="pyrF"
                     /locus_tag="AC16V7_00215"
     CDS             2325..>3354
                     /gene="pyrF"
                     /locus_tag="AC16V7_00215"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIR6255570.1"
                     /translation="MGFFSVIEARARQIDSLLCVGLDPHPSDLGAQTPEGASAFCLRL
                     IEATADLALAFKPNIAFFEALGAAGISALREIIATIPPGIPVILDAKRGDIASTAQAY
                     AKAAFEVLGVNAITLSPYLGYDSLEPFICDPQQGAFLLCKTSNPGAAELQDLTTISQS
                     LNPPLRLYERIAGLAQQWNTNDNLGLVVGATQPDALRRVRALAPDVWILAPGVGAQGG
                     DLAATLQAGLREDGLGLIIPVSRLLSRSDDPHKAAAHLVEEINQHRLRGNASGKRISS
                     THLRPTTPELIHGLLEAGCVQFGQFTLKSGMQSPIYIDLRRLVSYPGLLSLAAAAYLP
                     ILSSLKFDR"
BASE COUNT          755 a          964 c          882 g          753 t
ORIGIN      
        1 gaaaactact tgacagattc cacaataccc ttaaaatcgg gttgaatgga atactcttat
       61 gtccgtcatt tatcagggtt tcgccccgca gcgccagctg cgatgggcga aattttttta
      121 tatgatcaga gagccgtaca ggctctcttt ttttgtctgt gagaaatgca tgcagcttcc
      181 aggcctgatc gatccccatg tgcacatgcg cgagcccggc gctacccata aagaggactg
      241 gggtagcggc acatcagccg ccctggccgg cggatttact atcgtgctgg cgatgcccaa
      301 tacccagccg cctgtgacgg atcgcgattc cttctctatg gcactgaagg cagcccgcct
      361 caaagctctc tgcgattacg ctcaatacct aggagcagga ccagacaacg tggaggttct
      421 ccccagaatg gccttgcgct ccgctggatt gaaaatgtac ctggaccaga cctacgggcc
      481 gctgcgcctg gacgatatgg cgctttggat ggcacacttc gctcagtggc cgcgccagat
      541 gccgattgtt gcccacgctg aagggagcac gctggcagcg gtgatcctcc tggcgctgct
      601 atacgaccgc ccgcttcatc tggcacacgt ctcgcggcgg gatgaaatac tcttaattcg
      661 ggctgctaag gaacgaggca ttcgtattac ctgtgaggta gccccacacc atctcttttt
      721 gacccacgac gatctacctg gcggccgcgg cgaagtgcgc ccacgactgg cgaccgctgc
      781 tgatcgagac gcattgtggg cgaacatgga tgtcatcgat tgttttgcca cagaccatgc
      841 cccccatacc caggcggaga aagacagccc caacccaccc cctggctttc cgggcttaga
      901 gaccgccttg cctttgctgc ttactgcggt gaacgaaggc cgattgaaca ccgatgacct
      961 gctaacccgc atggtgaaca acccacgtcg cattttcaat ctgcccgagc aaccggatac
     1021 ctggatagaa gtggatacgg acgatgtttg ggaaatccgc gccgcagaca ctttcacccg
     1081 ctgcgcctgg acacccttcg aaggctggaa ggtgcgcggg cgggtgcgga aagtgacttt
     1141 gcgcgggcgc gaagtgtttc gagatggaaa aatcctggca cccgcgggat ctggaaagca
     1201 tatacgcgat gaaaaccgca gtctagcaag ataaggagat tttatgacca cttttagccc
     1261 accttcccga gtgcaaaatc catttttacc gttcggtgac cagcgcaaag ctcctttcta
     1321 tgggaaagat atcatctcgg tcaagcaatt ctctcgcccc gacctggatt atatcttcgg
     1381 cgtagcacat gaaatgcgcg agatggtcga gcgcatcggc acatttgatc tgttgaaagg
     1441 caagatcctc gccaacctgt tctacgagcc ctccacgcgc acttcttcgt catttacctc
     1501 agcgatggaa cgactgggtg gtagcgttat ccccattaac gaagtgcgct attcgtcggt
     1561 ctccaaaggc gaatcgctgc cagatacagt gcgcacttta gagtgctatg ctgacgtgat
     1621 tgtgctgcgg caccccgagg ttggcgcttc agccctggca gctcagtacg cacgcaaacc
     1681 aatcatcaac gccggtgacg gcatcggtga acaccccacc caggctctac tcgacctgtt
     1741 cactattatc gaagagctgg gacaggcgga cagcctgaca gtgactatgc tgggtgattt
     1801 aaaatacggt cgtacagttc actccctggc gcgcctgctc tctttataca atgtcagcct
     1861 gcattatgtc tcaccagata tcctgcgcat gcctactgag attattgccg aactcaaaga
     1921 gaagggcatc ccccaagtcg aacattccac tcttgatgag gtagtaccca agaccgacgt
     1981 gctttacgtc acccgtgtac aaaaagaacg ttttgctgac caggccgagt atgaaagcgt
     2041 aaagggtgcg tacgtcatta caccagatat catgagcaag gcaaaagagc gtatgatcgt
     2101 gatgcacccc ctgccacgag tcggtgagat cagcattgag tttgattctg acccgcgcgc
     2161 agcttacttc cgccagatgg aatacggatt gtacgtgcgc atggcattgc tggcaatggt
     2221 ttttggcaaa gcctagagat gaggtgagta gcctggtgaa tattcttgtg cgtcagttaa
     2281 tgcagggaaa tccagccatc aggaactgga caagggagtg agctatggga ttcttctcgg
     2341 ttatagaggc tcgcgcccgg cagatagatt cgctgttatg tgtaggactc gacccacatc
     2401 cctcggactt gggagcccaa acccctgagg gggcaagtgc tttttgcctg cgcctgattg
     2461 aagctaccgc tgacttagcc ctggcgttta agcccaacat cgcctttttt gaagcccttg
     2521 gagcagcggg tatttcagca ctgcgggaaa ttattgcgac gatcccaccc ggcatcccgg
     2581 ttatcctgga tgccaaacgc ggtgatatcg cttcaacggc acaggcctat gctaaggcag
     2641 ccttcgaggt gttaggcgta aacgccatca cgctaagccc ctacctcggc tatgattcgc
     2701 ttgagccgtt catctgcgat cctcaacaag gtgcctttct gctgtgcaag acttccaatc
     2761 cgggcgcggc agaactgcag gatctcacca ccatttccca atcactcaac ccacccctta
     2821 ggctatacga aagaatcgcg gggctggctc aacaatggaa taccaacgat aacttggggt
     2881 tggtggtggg tgctacccaa cctgatgcgc tcaggcgtgt gcgtgccctg gctcccgatg
     2941 tgtggatcct ggctccaggg gtaggagcgc aaggcggaga tctggcagct accctgcaag
     3001 ccggtctgcg agaggatggt ctgggactta tcatcccggt ctcgcgcttg ctctcccgca
     3061 gcgacgaccc acacaaagct gccgcccatc tcgttgaaga gatcaaccaa cacaggctaa
     3121 ggggaaatgc ttcaggcaaa aggatctcat ccacccatct tcgtcctact actcctgaac
     3181 ttattcacgg ccttctagaa gcgggttgtg tccagtttgg gcaattcacc ctgaaatctg
     3241 gaatgcaatc cccaatctac atcgacctgc gacgcctggt gagctatcca ggattgctct
     3301 ccctggcggc cgcggcctat ctgcccatct taagcagtct caagtttgac cgcc
//
LOCUS       DBNHOH010000013         2585 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1218264, whole genome shotgun sequence.
ACCESSION   DBNHOH010000013 DBNHOH010000000
VERSION     DBNHOH010000013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1218264"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..920)
                     /locus_tag="AC16V7_00220"
     CDS             complement(69..920)
                     /locus_tag="AC16V7_00220"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - (3S)-3-hydroxyacyl-CoA
                     dehydrogenase (NAD+) activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6255571.1"
                     /translation="MEIQRIFVIGAGTMGNGIAQVAATSGYPVTLMDVVPEQLERARA
                     TIAKSAEKLVSKGAVSEEQRAAATNIPFVSDLERVAEADLIIEAASENPELKFKIFRD
                     LDKQARPDAILASNTSSISITKIAAATHRPENVIGMHFMNPVPLMRLVEVIRGLATSD
                     QTTASTVEACRRMGKEPVEAKDSPGFISNRILCPMINEAIFALQEGIGTPEAIDTVMK
                     LGMNHPMGPLTLADLIGLDVVLSVMEVLQRDLGEDKYRPSYLLRKMVDAGHLGRKTGK
                     GFYSYSK"
     gene            complement(1015..2223)
                     /locus_tag="AC16V7_00225"
     CDS             complement(1015..2223)
                     /locus_tag="AC16V7_00225"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIR6255572.1"
                     /translation="MDNHDRQPVILSAVRTPSGRFQGSLSGFPAPKLGALAIKAAVER
                     AKINDPAQIEEVFMGNVVTAGEGQAPARQAAIFAGLPASVGATTINKVCGSGLKAVML
                     AAQAIKAGDGDLFVSGGMESMSRAPYLVDGRTGALRYGHVQLTDALLHDGLWDPFEDW
                     GMGNAAEFIAEEYEVTRQAMDEFALRSHQRALAASDAGKFKAEIVAVEIPGKKGQVTV
                     FDTDETPRRDTSLDVLASLKPAFQKDGKVTAGNAPGLNDGAAAVVVASRSKAEAMGAK
                     PLARIVGHAQAAVVPKDLFIAPAKAIPRLLDKVGWTLADVDLIELNEAFAAQVLADGY
                     ALADMGWDWDKVNVNGGGIALGHPIGASGARVLTTLIYALKDRGLKRGIASLCLGGAE
                     AVAMAIEIED"
     gene            complement(2272..>2585)
                     /locus_tag="AC16V7_00230"
     CDS             complement(2272..>2585)
                     /locus_tag="AC16V7_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013401.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HIR6255573.1"
                     /translation="SNEHKSTLDELDAETRAEIMELSNLATRALQAVYNPHGFNLGLN
                     IGDAAGAGILEHVHMHILPRWKGDTNFLSALDETRVIPEALEETYKRLKKGLEDILRS
                     R"
BASE COUNT          500 a          773 c          674 g          638 t
ORIGIN      
        1 ccccgaaaac gggggagggg ttgggggtgg gggcattcaa tttggttatc caaccgattg
       61 ctgataattt attttgaata actatagaat ccttttcctg tcttccttcc caaatgcccg
      121 gcatccacca tcttgcgcag cagataagat ggacgatact tatcctcccc aaggtcccgt
      181 tgtagaacct ccattaccga aagcaccacg tccagcccga tcaaatcagc cagcgtaagc
      241 ggtcccatcg gatggttcat gcctagcttc atgacagtgt cgatagcttc cggggttcca
      301 attccctcct gcaaggcgaa gattgcctcg ttgatcatcg gacaaaggat gcggttagag
      361 ataaacccgg gtgagtcttt ggcttccacc ggctctttac ccatccttcg acaggcctcc
      421 acagtggagg ctgtggtttg atcagaagtc gccaaaccgc ggataacctc caccagccgc
      481 ataagaggca ccgggttcat gaagtgcatg ccgatcacat tttcggggcg atgggtcgcc
      541 gcagcgatct ttgtgatact gatcgaggat gtgtttgagg ccaggatggc gtccgggcgg
      601 gcttgtttat ctaggtcgcg aaatattttg aacttcaact cggggttctc gctggcagcc
      661 tcgatgatca ggtcggcttc tgccacacgc tctagatcac tcacaaaagg gatatttgtc
      721 gctgctgctc tttgctcttc gcttacagct cccttgctga ccagtttttc tgccgatttg
      781 gcaattgtgg cgcgggcacg ttccaattgc tcgggcacta cgtccattag agtcactggg
      841 tagcccgacg ttgccgccac ctgggcgatg ccattgccca ttgttcctgc cccgatgaca
      901 aagatacgct gaatttccat agactagctc cttagatgaa aaggggagaa atcgatgggg
      961 ggtatctatc gtgctctgct gctttctcta acccctatcc cctctcccca atatttaatc
     1021 ttcgatctct attgccattg ccaccgcctc cgcccctccc aggcacaagg aggcaatccc
     1081 gcgcttcaga ccgcggtctt tcagcgcgta gatcagggtg gtcagcacgc gtgccccgct
     1141 agctccaata gggtgtccca aggcgatacc cccgccgttc acgttcacct tgtcccagtc
     1201 ccagcccatg tctgccaaag catagccatc agccagcacc tgcgcggcga aggcctcgtt
     1261 tagttcgatc aggtcaacgt cggctagcgt ccatcccacc ttatcaagca gacgagggat
     1321 ggcttttgca ggggcgatga aaagatcctt gggcacaaca gccgcttgag cgtgccccac
     1381 aatgcgcgcc agcggctttg cacccattgc ttctgctttg gagcggcttg ccaccaccac
     1441 agccgctgcc ccgtcgttca gcccgggggc gtttccagcg gtgactttgc cgtctttttg
     1501 aaaagctggc ttcaagctgg cgaggacatc tagcgatgtg tcccgccggg gcgtctcatc
     1561 ggtatcgaag accgttacct ggcctttctt gccgggtatc tcgacggcta caatctcagc
     1621 tttaaacttt ccagcgtcgc tggcagcgag tgctctttgg tggcttcgca gggcgaactc
     1681 gtccattgcc tggcgggtga cttcgtattc ctcggcgata aactcggctg cattgcccat
     1741 cccccaatcc tcaaatggat cccacaagcc gtcgtgtaac agggcatcgg tgagctggac
     1801 atgcccgtaa cgcagtgcgc cggttcgccc atccaccaga tagggtgccc ggctcatgct
     1861 ctccatcccg ccggacacaa acaggtcgcc gtcgccggct ttaattgctt gtgctgccag
     1921 catcacagct ttcaggcctg aaccacacac tttgttgatt gtggttgccc ctacactggc
     1981 tggcaagccg gcaaaaatcg ctgcctggcg ggctggcgct tgcccttccc cagccgtgac
     2041 cacatttccc atgaagacct cttcgatttg ggcggggtca ttgatcttag cgcgctctac
     2101 ggctgctttg attgccaatg ctcccagctt gggagcaggg aagccactca ggcttccttg
     2161 gaaacgaccc gaaggcgtgc gtactgcgct caggatgacg ggttggcgat cgtgattgtc
     2221 catgtggtag ccctccgtgt tgcggattat gtcgttatgg atccgtgttt gttacctact
     2281 gcgcaatata tcctctaacc cctttttcaa ccgcttatat gtctcttcca aagcctctgg
     2341 gatgacacgc gtttcatcca atgctgacaa gaagttcgta tcccctttcc agcgcggcag
     2401 gatgtgcata tggacgtgtt cgagtatgcc tgctccggcc gcgtcgccga tattcaaccc
     2461 caggttgaaa ccgtgaggat tgtatacggc ttgcagcgcc ctggtcgcca gattggacag
     2521 ctccatgatc tcggcacgcg tttcggcatc cagctcgtcc aacgtggatt tatgctcgtt
     2581 agaga
//
LOCUS       DBNHOH010000014         3816 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_513404, whole genome shotgun sequence.
ACCESSION   DBNHOH010000014 DBNHOH010000000
VERSION     DBNHOH010000014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_513404"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2587
                     /locus_tag="AC16V7_00235"
     CDS             <1..2587
                     /locus_tag="AC16V7_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR6255574.1"
                     /translation="LETVGGKGASLARLAGAGFPVPGGFHVTTQAYRQFVSANDLQTR
                     ILAALEEVDISIPNTLEKASHTIREAFLQGQIPPEIANDIVQSYAELPGTNPAVAVRS
                     SATAEDLPDASFAGQQETYLNVSGAGAVLEATKKCWASLWTARAIGYRARQGIHAEGV
                     ALAVVVQLLVPAEAAGILFTANPMNGRRDQVLISASWGLGEAVVGGLVTPDSLIMDKA
                     SGNVLERQTADKQVMTVRVDGKTEEQPIPENLRRIPVLDDEIATELTRLAVQIEDLYG
                     MPMDIEWTWTDGEFSIVQARPITALPTSETLASVEWTLPDPKGQYMRTSIIDFLPDPL
                     TPLFATLGVSELNVGIKRLIAYMVGKETQDANWPDDSILTINDYAYFAAKYTPRQMLW
                     IIFVLVPKFPSLLRSGIPRWRDEVRPYYLKTVERWEDKPLVSLTPSEIFSGVHEVMEA
                     AIDHLGSLMVGTMGVAAGTEMLFTRVYEKMIKKDGDLSAPAFLMGYDSIPIRSEKTLY
                     DLAKWSRNIPDLAVYLSETPTLQIRAALDSNIAPLSVNVDTWHEWQERFQSYLDAFGH
                     LIYDLDFAKPLPKDDPTSMLETIKMYLRGEGTNPHERQQVAAERRLQASENTLRRLKG
                     LRRWAFRTTLKLAQRQAEVREDGIADIGLGYPLLREMLFELGSRLVEAGAIQQADDIF
                     WLEQAEIETLIAAMEQGSAINSLADSMQERKALWKSKKLLVPPPTLPPTKKYMGFDIE
                     AFVGAGEIGQVGDTLKGVATSAGRVTGTARVLHGPQDFDQMQAGDILVAKITTPAWTP
                     LFAMASAVVTDIGGPLSHGSIVAREYGIPAVLGTGVATHRISSGQKIAVDGDAGTVTL
                     LNGHE"
     gene            2621..>3816
                     /locus_tag="AC16V7_00240"
     CDS             2621..>3816
                     /locus_tag="AC16V7_00240"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6255575.1"
                     /translation="MMVNSAKSGRRQIYILAFVLLVVMLGFGVVIPIIPFFIEDMGAG
                     GTELGLLVASYAIMRLIFGPIWGSLSDRVGRKPILMVGIFGYGITMILFGLATKLWML
                     FVFRSLSGILSSATSPTTMAYISDSTSEKDRGGGMGTLGAAIGIGTILGPGFGGLLGG
                     ESLSIPFFIAGGMSFLARLLIWLFLPESLSVEARRQASHKKRLTASDLWQAMVGPIGI
                     LLGMAVLVSYAMTTFYSIFGLYALQKFSYGTQEVGAIMMVVGMVSALAQGVFTGPLTR
                     RWGEAAVIKTTLLATALGFIGISLGNTFVTFLIAIGFFTLATALLTPAITALTSKRTT
                     LEQGISMGLSNSFMSLGRIAGPMVAGITFDINIEYPNLSGALVLLVGFLASLVWVKKA
                     APTPKTA"
BASE COUNT          820 a         1060 c         1100 g          836 t
ORIGIN      
        1 cctggaaact gttggcggca agggcgcctc gctagcccgg ctcgctggcg caggtttccc
       61 tgtgcctggg ggattccacg tgaccacaca ggcttatcgc cagttcgtgt ctgcaaatga
      121 tttgcaaacg cgcatcctgg cagctttaga agaagtggat atatccatac caaatactct
      181 tgagaaggca tcccatacca tccgggaagc gttcctccag gggcaaattc ccccagaaat
      241 cgccaacgat attgtccagt catatgctga gcttcccggc accaacccgg cagtggcggt
      301 gcgttcgtcg gcgaccgctg aagacctccc cgatgcctct tttgccgggc agcaagagac
      361 ctatctcaac gtcagtggag cgggggccgt gctagaggcg accaagaagt gctgggccag
      421 cctatggaca gcacgcgcaa tcggctaccg agcccggcag gggattcatg cagagggcgt
      481 ggccttagca gtggttgtgc agctgctcgt cccagccgaa gctgctggga tcctgttcac
      541 tgccaatcct atgaatggac ggcgcgatca ggtcctgatc agcgcctcct gggggttggg
      601 ggaagcagtt gtcggagggc tggtcacacc tgactcgctg atcatggaca aagcctccgg
      661 gaatgtgctg gagcgccaaa ccgccgataa gcaggtgatg accgtgcgtg tggatgggaa
      721 aaccgaggaa cagcctatcc cagagaacct gaggcgcatc cctgttcttg atgacgaaat
      781 agcaactgag cttacccgcc tggcggtcca gatagaggac ctgtacggca tgccgatgga
      841 catcgagtgg acatggacgg atggggaatt ctccatcgtc caggcacggc ctatcacagc
      901 cctgccgacc agcgagacct tagcctcggt tgaatggacc ttgcctgatc caaaaggtca
      961 gtacatgcgt accagcatca tcgacttttt gcccgatccc ctgacgccgc tctttgctac
     1021 attaggggtt tcagagctca atgtcggcat taagcgcctg atcgcttata tggtcggtaa
     1081 ggaaactcaa gacgcaaact ggccagatga ttcgatactg accattaatg attatgcata
     1141 ctttgccgcc aaatacacac cgaggcaaat gctctggatt atttttgtct tggtgccaaa
     1201 attcccgagc ttgctgcgca gcggcatccc gcgttggcgc gacgaggtga ggccatatta
     1261 tctgaaaacc gtagagcgct gggaggataa gcctttggtg agtcttaccc caagcgagat
     1321 tttctccggg gtgcatgagg ttatggaggc tgcgatcgat cacctcggct cgttaatggt
     1381 tgggacgatg ggcgtggcgg ctggcactga aatgctgttt acacgcgtct acgaaaaaat
     1441 gatcaagaaa gatggcgacc tatccgcgcc agccttcctg atggggtacg atagcatccc
     1501 catccgctct gaaaagactc tgtacgacct ggctaagtgg agccgcaaca tccctgacct
     1561 ggcggtttat ctcagcgaga cgcctaccct tcaaatccgg gccgcgctcg acagtaatat
     1621 tgcaccccta agcgtgaatg tagatacctg gcatgaatgg caggaacgct tccaaagtta
     1681 cctggatgcc tttggacacc tgatctacga tctggacttc gccaagccat taccaaaaga
     1741 tgacccaacc tccatgctgg agacaatcaa gatgtatctg cgtggcgaag gcaccaaccc
     1801 gcatgaacgc cagcaggtcg ccgctgagcg acgcctgcaa gccagcgaga acacacttag
     1861 gcggctgaaa ggcttgcgcc gttgggcttt ccgcacaaca cttaagctgg cccaacgcca
     1921 ggccgaggtg cgcgaggacg gcatcgcaga tatcggtctg ggatacccgc tgctacggga
     1981 gatgctgttc gagttgggca gccgcctcgt tgaagcaggg gctatccagc aagccgatga
     2041 cattttctgg ctggaacagg cagaaattga aaccctgatc gcggcgatgg agcaaggaag
     2101 cgcgataaac agcctagctg attccatgca agagcgcaaa gcgctctgga aatccaaaaa
     2161 actcctggtg ccaccgccga cgttgccgcc aaccaagaaa tatatgggct tcgacatcga
     2221 agcgtttgtc ggggcaggag aaatcggtca ggtgggcgat acgctgaagg gcgtggctac
     2281 cagcgctgga cgtgtgaccg gcaccgcgcg tgtgctgcac gggccgcagg attttgacca
     2341 gatgcaagca ggggacatct tagtagccaa gataaccacc cctgcctgga cgccactctt
     2401 tgcaatggct tctgctgtgg tcaccgacat cggtgggcca ctcagccatg gttccatcgt
     2461 cgctcgcgaa tacggcatcc ctgccgttct tggtacgggc gtggctacgc accgcatctc
     2521 cagcggtcag aagatcgcag tggatggcga cgctgggaca gtgacgctgc taaacggtca
     2581 cgagtagatc aggcgcatcc atataagctc gtaaaggtac atgatggtta attcagccaa
     2641 atcaggtcgt cgccaaatat acatcctggc atttgtgctc ctggtcgtta tgttggggtt
     2701 cggtgtcgtc atccccatca tccctttttt catcgaggac atgggtgcgg gcggcaccga
     2761 gctaggactg ctggttgcca gctatgccat catgcgcctg atcttcgggc ctatctgggg
     2821 tagcctttct gaccgcgtgg gacgcaaacc gatcctgatg gtgggtatct tcggctatgg
     2881 gattaccatg attctgtttg gattggcgac aaaactgtgg atgctgttcg tcttccgctc
     2941 actctcgggt attctctcgt ctgctacctc accaaccaca atggcttata ttagcgacag
     3001 cacatcggag aaggatcgcg gtggggggat gggcacgctg ggcgccgcca ttgggatcgg
     3061 caccatcctg ggaccaggct ttggcggttt actgggcgga gaatcactgt ccataccgtt
     3121 tttcatcgcc ggcgggatgt ctttcctggc ccggcttttg atttggctgt ttctgccaga
     3181 gtcattgtcg gtagaagctc gccggcaggc cagtcacaaa aagcgcctca ccgccagtga
     3241 tctctggcaa gccatggttg gcccaatcgg catcctgctg gggatggccg tcctggtctc
     3301 atacgccatg actactttct atagcatatt cggtctgtat gccttgcaga agttcagcta
     3361 tggcacccag gaggttgggg cgatcatgat ggttgtgggg atggtttccg ccctggctca
     3421 gggcgtcttt accggcccgc tgaccaggcg ctggggagag gccgccgtca tcaagaccac
     3481 tctgctcgcc accgctcttg gtttcatcgg catctccctg gggaacacct ttgtaacctt
     3541 tctgatcgcc attgggttct tcacactggc aaccgccttg ctgacaccag ccattactgc
     3601 gctgacatcg aagagaacca ccctcgagca agggatcagt atgggcttga gcaactcctt
     3661 catgagcctg gggcgtatcg ctgggccaat ggttgcaggg atcacctttg atataaatat
     3721 cgaatacccc aacctgagcg gagcgttggt tttgctggtc ggtttccttg ccagcctggt
     3781 ttgggtgaag aaggctgcgc ctaccccaaa aacggc
//
LOCUS       DBNHOH010000015         3515 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1667052, whole genome shotgun sequence.
ACCESSION   DBNHOH010000015 DBNHOH010000000
VERSION     DBNHOH010000015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1667052"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="AC16V7_00245"
     CDS             <1..1019
                     /locus_tag="AC16V7_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255576.1"
                     /translation="LLGGGNRPAWSPDGDIVLTTLPTPNRTYLTAYRADASGLALPPL
                     PLPGQPAGLTWGRVSLPQPLPSFLSQAARITTTPAANAQLTPATNIPAGRQRVVELED
                     VQAPYAKLHDSVDESFNALRLEVASRLGWDFLATLENAYVPLTSPLFPGLLNDWLYTG
                     RAIVINPAPINAGWMVIMRDDFGPTTYWRVYLRTRFQDGSQGMPLHDIPWDLNARYAG
                     DPRDYEQGGSLAPAIPPGYWLDFTNLAASYGWERLPALSTWRSALPAARYNEFVHTDD
                     LDWFSAMVEIYPIEAVYTPTPIQPPTFTPTPTRRPTRTPTPTRTPTITRTPMPLQQTI
                     SPTP"
     gene            1163..2194
                     /locus_tag="AC16V7_00250"
     CDS             1163..2194
                     /locus_tag="AC16V7_00250"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR6255577.1"
                     /translation="MNSIPSPTASPPTPTPLTETEDLPETNLGLLALTMPTPAPDPLR
                     FSFPTPGSPPVSAWRPPLYPVPWVPTPFDHFYFVRPIAADEVNWPLADYRYGGVFLPE
                     VVHTGIDIPAPSGTPVLAAGPGRVTWAGYGLYRGVEDTTDPYGLAVAIKHNFGYNGET
                     LYTVYGHLQRIDVLVGQQMNTGDAIGLVGDTGKVTGPHLHFEVRLGRNNFFESRNPEL
                     WLAPPEGWGILAARLTNTRGERLYSHLISVHSKSGTRFWAVKSYGQGAAISDDYYQEN
                     LVLGDLPAGDYVVWIPFEGSTYNLDVTISPGRVTFFRFRGRNGFDSGLPPTPKPDFTP
                     PAAQISPTP"
     gene            2293..3375
                     /gene="recA"
                     /locus_tag="AC16V7_00255"
     CDS             2293..3375
                     /gene="recA"
                     /locus_tag="AC16V7_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR6255578.1"
                     /translation="MNRKNTSSSRNQDDSRSIALEKALGDITKRYGDGTIMRLGEAHK
                     LMVEAIPTGSLSLDIALGVGGIPRGRITEVYGPESSGKTTICQHIVAEAQKLGGTCAF
                     IDMEHALDPTYAEHCGVDVDSLLISQPDMGEQALEITETLVRSGAIDVIVIDSVAALV
                     PRAEIEGDMGDPQMGMQARLMSQALRKLSGAIKQTNTAVVFTNQLRQKIGVMFGNPET
                     TTGGMALKFYASVRLDVRRIQSIKLGAEIIGNRTRVRVVKNKVAPPFRTAEFDIMYNE
                     GISKVGDLIDLATELEIITKRGSFYSYGDIRLGQGRENAKDFLRQNPEMADEIELAIR
                     QQSLSGVMPLPFGGEDDEGGLDDDEA"
BASE COUNT          782 a         1097 c          912 g          724 t
ORIGIN      
        1 acctgctggg aggcgggaac cggcccgcct ggagtccgga tggggacatt gtactcacca
       61 cgcttcccac cccaaaccgc acctatctca ccgcctatcg agctgatgct tccggcctgg
      121 ccctgccgcc gctgcccttg cctgggcagc ctgccggatt gacctggggg agggtctcac
      181 tacctcagcc actcccctcc ttcctgagcc aggctgcacg cattacgact acccctgcgg
      241 caaatgctca actgacacct gcaaccaaca tacccgccgg tcgacaacgg gtcgtagaat
      301 tagaagacgt tcaggcacca tacgccaagc ttcacgatag cgtagatgaa tccttcaatg
      361 cattacggct agaagtggcc tctcgcctgg gctgggattt cctcgccacc ctagaaaacg
      421 cctacgtccc gctgacttcg ccactcttcc ccggcttgct caacgactgg ctgtatacgg
      481 gtagagccat agttatcaat cccgcgccga tcaatgctgg ctggatggtg atcatgcgcg
      541 acgattttgg acccaccact tactggcgcg tgtatttgag gacgcgcttc caggatggat
      601 cacagggcat gccacttcac gatatacctt gggatctgaa cgcgcgctat gctggcgacc
      661 cacgcgacta tgaacaaggt ggcagcctcg caccggcaat ccctcccggc tactggctgg
      721 acttcaccaa cctggccgcc agctatggct gggaacgtct ccctgcgctg tccacatggc
      781 gctcagctct gccagcagcc cgctataacg agttcgtaca caccgatgac ctggattggt
      841 tctctgcgat ggtagaaatc tatcccattg aggctgttta tactcccacc ccaatacagc
      901 cccccacctt tacccccaca cccacacgcc gtccaacacg cacccccacg cccacccgca
      961 caccgacaat tacacgcaca cccatgccgt tacagcagac catctcccca acaccatgag
     1021 atcctctgct cgcaatacta aaaccatttc caggtggaaa aaatcagcca gatggctact
     1081 gctgctctgg ctggccgcct gccaatcccc ttggtcagca cctccgcctc ccaccgagat
     1141 ggaacccacg cctgaaatcg aaatgaattc catcccatct ccgacggctt cacccccaac
     1201 acccaccccg ctgacggaaa cggaagatct gcccgagaca aacctgggat tgctggcgct
     1261 gaccatgccc acgccagctc cagatccatt gcgtttctcc ttccccacgc ccggatcccc
     1321 accagtctca gcatggagac caccgcttta ccccgttcca tgggtgccaa ccccttttga
     1381 ccatttctac tttgtgcgcc ccattgccgc cgacgaggtg aactggcctc tggctgatta
     1441 ccgttatggg ggtgtattcc tgcccgaggt cgtgcatact ggcatcgata tacccgcgcc
     1501 cagcggcacc ccagtgctgg cagccggccc tggaagggtc acctgggcag gctacggtct
     1561 ctatcgaggc gttgaagaca cgaccgatcc ctatgggctg gcagtcgcta tcaaacacaa
     1621 ctttggttac aacggcgaga cgctgtatac tgtctacggc cacctgcaac gcattgacgt
     1681 gctggtaggg cagcaaatga acaccggcga cgccattggg ctggtgggcg ataccggaaa
     1741 agtcaccggc ccgcacctgc actttgaggt gcgactaggt agaaacaact tcttcgaaag
     1801 ccgcaatcca gaactatggc tagccccgcc tgagggctgg ggcatcctgg cggcacgtct
     1861 gacaaatacc cgtggtgaac ggctctacag ccatttgatc agcgttcact ccaagtcggg
     1921 cacgcgtttt tgggcggtca agtcttatgg acaaggggca gcgatcagcg acgactatta
     1981 ccaggagaat ttagtattag gcgacctgcc tgctggggat tatgtggttt ggatcccctt
     2041 tgaaggcagc acctataatc ttgatgtgac catctcgcct gggagggtca cgttcttccg
     2101 atttcgcggg agaaatggct tcgatagcgg gctaccacct acgccgaagc cggattttac
     2161 ccctccggca gcgcaaattt ctcctacccc ttgacaggtc gaacatttgt gctaaactat
     2221 gcgcatgcca ctgcggcaaa cttatctata agtttgatct ttagaatttc agccagcagg
     2281 agtttttgat ccatgaaccg taaaaacact tcatcctctc gcaatcaaga cgattcacgc
     2341 agcatcgccc tcgagaaggc tctcggcgac atcaccaaac gctacggcga tggtacgatc
     2401 atgcgcttgg gcgaggcgca caagcttatg gtagaagcca tccccaccgg ctccctttct
     2461 ctcgacattg ccttaggcgt aggcggcatc ccgcgcgggc gtattaccga agtatatgga
     2521 ccagagtcct cgggtaaaac caccatctgt caacatatcg ttgccgaagc acagaagctg
     2581 ggcggaactt gcgccttcat cgacatggag catgcccttg accccaccta tgcagagcac
     2641 tgcggggtcg atgtggatag cttgctgatt tcacagcccg acatgggcga gcaagccctg
     2701 gaaatcactg aaaccctggt gcgctcgggg gcgatcgatg tcatcgtgat cgactcggta
     2761 gctgcactgg tgccgcgcgc tgaaatcgag ggcgacatgg gcgaccccca aatgggcatg
     2821 caagctcgat tgatgtcgca agcattgcga aaattgtccg gcgcgatcaa gcagaccaac
     2881 accgcagtgg tattcacgaa ccagctgcgc cagaagatcg gggtgatgtt tggcaacccc
     2941 gagaccacca ccggcggcat ggcgctcaag ttctacgctt ctgtccggct ggacgtgcgc
     3001 cgcatccaat ccatcaagct gggggcagag attattggta atcgcacgcg cgtgcgcgtg
     3061 gtgaagaaca aagtcgcccc gcccttccga accgccgaat tcgacatcat gtacaacgaa
     3121 ggaatatcca aggtcggaga cctgattgat ctggctaccg agttggagat tatcaccaag
     3181 cggggatctt tctattcata cggagatatc cgcctgggtc aaggccgcga aaatgccaag
     3241 gatttcctgc gccagaaccc cgaaatggct gacgagatcg agctggcaat ccgccagcag
     3301 tctttgagcg gagtaatgcc actgcctttc ggaggagagg atgacgaggg cgggttggac
     3361 gatgacgagg catagaccga accttgcgat ctccggatag cttgcctgat cgaagatgat
     3421 cggaatttta cgcttacgca tcctgctaat ctatctttgt ctgatgggga tagctgcagg
     3481 atgcagtgct ttgtcgcaag ataagcagga agctg
//
LOCUS       DBNHOH010000016         3204 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1090179, whole genome shotgun sequence.
ACCESSION   DBNHOH010000016 DBNHOH010000000
VERSION     DBNHOH010000016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1090179"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="AC16V7_00260"
     CDS             <1..330
                     /locus_tag="AC16V7_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HIR6255579.1"
                     /translation="IAADLIREASLLILRDEVPHGIAVRIDQFTERGETGAYIEATLL
                     VEKETHKGIVIGQGGSMLKEIGSRARAEIEKMSGRKVFLRLRVKVRKNWRNDDRVLKS
                     FGYTKRN"
     gene            complement(459..1499)
                     /locus_tag="AC16V7_00265"
     CDS             complement(459..1499)
                     /locus_tag="AC16V7_00265"
                     /inference="COORDINATES: protein motif:HMM:NF053377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF8173 domain-containing protein"
                     /protein_id="HIR6255580.1"
                     /translation="MKTNLRIAILLTLVLVLILPSTAMARGLFGDRIVFGDTFTLESG
                     ETLDGNLIVFGGNAILEVGSQVDGDVVLMGGNLEADGSIGGSVVAIGGLVSLGETSLV
                     EGDVAAIGAQLDKAEGARIIGEVINNLDTPLVFPFTDSVRVPRFEVNFSPIVNIVMFF
                     MKVLLWSALAVLVVLFMPEYTQRVGQAAIAQPPLAGGLGLLTVVALPVVIIALTITIL
                     LIPVSLLVIVLFGLAWIFGLIGLGLELGKRLAELTKQEWAPAVSSGVGTLVLILVLNG
                     VREVIPCIGWIPSALAGLLGLGAVLLTRFGSQAYQPQGVGAQIPAPPSAPQPPEAPMP
                     PQPETDEETSSS"
     gene            complement(1496..2044)
                     /locus_tag="AC16V7_00270"
     CDS             complement(1496..2044)
                     /locus_tag="AC16V7_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR6255581.1"
                     /translation="MVKPNHQPFENWLLSEGALTQEESQTLQEHLNDCETCRMLSEAL
                     RDVDRELRTASVLSPAPGFVSRWQARLEADRLRLYRRQTFFIMILSIGGAMFLLTWLA
                     IVLYPLIQSPKPVLLAVVYELTSTFTFATAIGEALMTVLRTVVGVIPPTQWAAIWTAL
                     AGLGALWFIALQRLTRPRRIIV"
     gene            complement(2016..2618)
                     /locus_tag="AC16V7_00275"
     CDS             complement(2016..2618)
                     /locus_tag="AC16V7_00275"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6255582.1"
                     /translation="MNDTESSWLAQAQKGDAEAFSRLIETYQRPVYNLCYRMLNDAGD
                     AEDAAQETFLRAYKSMKRYDQSRPFPTWLLSIAAHYCIDQIRKRRMAIVSTEDLPYPD
                     LSDDAPGPESVVSRKEEQQRVRVLLDTLSPTDRAAVVMYYWYDFSYEEIGEALSLTLS
                     AVKSRLHRARLSMAQEWIDQQPQAGLAERKQHGKAQSPAF"
     gene            complement(2702..>3204)
                     /locus_tag="AC16V7_00280"
     CDS             complement(2702..>3204)
                     /locus_tag="AC16V7_00280"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026183.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6255583.1"
                     /translation="GTLAIMVGGPAEALDRVLPIFEAMGKTITHVGGAGTGQIAKAAN
                     QIMVAAQMVAMGELLVFAQKAGADPRKVVEAIQGGAAQCWTLDVKPQRLFAGNRQPGF
                     KAYMQAKDLGIVMESARQYGIPLPAAAVHTQLFNAMLQMGMSELDNSAVLAVIEQLAG
                     IRLSEG"
BASE COUNT          811 a          927 c          800 g          666 t
ORIGIN      
        1 attgctgcag acttgatacg cgaagcgtcg ctgctcatct tgcgcgatga ggtgccacac
       61 ggaatcgctg tacgcataga tcaatttacc gagcgtggtg agaccggagc ctacattgag
      121 gccaccctgc tcgtcgaaaa agagacccac aagggcattg taattggtca ggggggcagt
      181 atgctcaaag agatcggcag ccgggcaaga gctgagatcg agaaaatgag cgggcgcaaa
      241 gtcttcctca gactgcgggt caaagtgcgc aaaaactggc gcaatgacga tagggtcttg
      301 aaatcttttg ggtacaccaa gcggaattga gcgaaatttg tgatggcaag accaaatatt
      361 tgagagagct aacaacacac ccctccctgt tatcttggga ggggtttgtt gtcacccact
      421 atcttgagta agcgtttagc aatcagcgaa tatgcccgtc acgagcttga ggtctcctca
      481 tccgtttccg gctggggagg catgggcgct tctggtggct gcggtgcgct gggaggcgcg
      541 gggatttgcg ctcccacgcc ctgcggctgg taagcctgtg acccaaagcg ggtgagcaag
      601 acagcgccta accccaataa gccagccagg gcagaaggta tccagcctat gcatgggata
      661 acctcccgca caccattaag caccaatatc aacaccaggg ttcccacccc agatgaaact
      721 gcaggagccc attcttgctt ggtgagctca gccagacgtt ttcccaactc aaggccgagg
      781 ccgatcaggc caaatatcca ggctagtcca aataatacaa tcactaatag gctaaccggg
      841 atcagtaaga tggtgatcgt caaggcaatt atcaccaccg gtaaggcaac tactgtcagc
      901 aaacccaggc ctcctgccaa agggggttga gcaatagcag cttgacctac acgttgggtg
      961 tactccggca tgaacaaaac caccaatacc gccagagctg accagagcaa gactttcata
     1021 aaaaacatca cgatgtttac tatgggagag aaattcacct caaagcgggg gacacgaaca
     1081 ctgtcggtga atggaaagac aagcggtgtg tccaggttgt tgatcacttc accgataata
     1141 cgcgccccct cggctttatc caattgggcg ccaatggccg cgacatcccc ctccaccaga
     1201 gacgtttctc ccaaggagac cagcccaccg atagcaacca ctgacccgcc aatgctgcca
     1261 tcagcctcca ggttgcctcc catcaaaacc acatcaccat caacctggga acctacctcc
     1321 aagatggcat ttcccccaaa gacaatcagg tttccatcca gcgtttcacc actctcaagg
     1381 gtgaatgtat ctccaaatac gatcctgtca ccgaacaacc cgcgtgccat cgccgtgctc
     1441 ggcaaaatga ggaccaaaac cagggtaagc aatattgcga tacgtaagtt tgttttcata
     1501 caattatcct ccgcggcctt gttagccgtt gtaaagcaat aaaccaaagc gcgcccagac
     1561 ccgcaagcgc tgtccatatg gctgcccact gtgtgggcgg aataaccccg accaccgtgc
     1621 gtagcaccgt catgagcgcc tccccaatcg cggtggcgaa tgtaaatgta gaagtaagct
     1681 cataaaccac agccagcaaa accggcttgg gagattgaat aagcgggtag agcacgatgg
     1741 ccaaccaggt caacaaaaac atcgccccgc caatgctcaa tatcatgata aaaaaggttt
     1801 gcctgcggta taagcggagg cgatctgcct ccaaacgcgc ctgccagcgg cttacaaaac
     1861 ctggagcagg cgacaaaacc gacgcagtcc gcagctcacg atcgacatcc cggagtgcct
     1921 ccgaaagcat tcggcaggtc tcacaatcat ttagatgctc ctgcaaggtt tgtgactcct
     1981 cttgggttaa cgccccttca gagagcaacc aattttcaaa aggctggtga ttgggcttta
     2041 ccatgttgct tcctttcggc caaaccggcc tgtggttgct ggtcgatcca ttcctgtgcc
     2101 atgctcaacc gagcgcgatg caggcgactc ttgacggcgc tgagcgtgag cgataatgct
     2161 tcaccaatct cttcatagga aaagtcatac cagtaataca taactacagc agcacgatcc
     2221 gtggggctca aggtgtccag caggacacgc acccgttgct gctcctcttt gcggctcacc
     2281 acagattccg gacctggggc atcgtccgat agatccgggt agggcaagtc ctcggtagat
     2341 acaatcgcca tcctgcgctt gcgaatctga tcgatgcagt aatgcgcagc aatggaaagc
     2401 aaccaggtag ggaaaggacg gctttgatca tatcttttca tcgatttgta tgctcgcaag
     2461 aatgtctctt gcgcagcatc ctcggcatcg cctgcgtcat tcaacatgcg gtaacacagg
     2521 ttataaactg gcctctggta ggtctcgatc aagcggctaa aggcttctgc atcgcctttc
     2581 tgagcctggg cgagccagct tgactcggta tcattcactg caatgctcct gtggcgcgca
     2641 ttccgcgcct gaactattca actgtatata cgcagattac aacttcaggt tgcaaaataa
     2701 tttacccctc cgaaagacgg atcccagcga gttgttcgat cacagccaat accgccgagt
     2761 tatccagctc agacataccc atctgcagca tggcattgaa gagctgcgtg tgcacagccg
     2821 ctgcaggcaa aggtatgcca tattggcggg cggattccat cacgatgccc aaatctttgg
     2881 cctgcatgta tgccttaaag cccggctgtc ggttgccggc aaacaagcgc tgcggtttga
     2941 catccagcgt ccagcactga gctgcaccac cctggatggc ctccacgact ttgcgcgggt
     3001 ccgcgccagc tttttgggca aaaacaagca attcgcccat cgccaccatc tgggcagcca
     3061 ccatgatctg gttggcggcc ttagcgatct ggcctgtgcc agccccacct acatgagtga
     3121 tggtcttgcc catcgcctcg aagattggca agaccctatc cagagcctcc gctggccctc
     3181 ccaccataat cgccaacgtc ccct
//
LOCUS       DBNHOH010000017         2456 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1731165, whole genome shotgun sequence.
ACCESSION   DBNHOH010000017 DBNHOH010000000
VERSION     DBNHOH010000017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1731165"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="AC16V7_00285"
     CDS             <1..1215
                     /locus_tag="AC16V7_00285"
                     /inference="COORDINATES: protein motif:HMM:NF017072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-fold NAD(P)-binding domain-containing
                     protein"
                     /protein_id="HIR6255584.1"
                     /translation="QQQGWLLTSQVKHPSLFYLRGATISLPKSIGFPLMMKEPGEKRV
                     FLPEFIQYLSESGLIIFIEESYGSRSGWSFDDYKQGNENVRSCSQLEAFQQDIVVVLR
                     SPTFEEFQLLRPGTNLISMLHFPTRPKRVEALKELGVDAISLDSIVDDNNIRLVENMR
                     AVAWNGLEAAFDVLENRWPGLRRPDGQPIRVLILGTGMVGKHASEAATKLGNIERNNQ
                     HIEDGGPGSVAISIGRNITHHPERLKKLFNEADILVDATQRRDTSVPVVPNAWIEWLP
                     QHAVITDLAVDPYTLDTQPPVVRGIQGIPKGNLDQYVFYPDDPNWEKTVPTTVPNQHR
                     RTVVSCYSWPGIHPEACMRHYALQLDPLMEVLITKGYDGLDLHGGYFERAMYRGTLRT
                     WLNSGHYEPRPR"
     gene            complement(1252..2106)
                     /locus_tag="AC16V7_00290"
     CDS             complement(1252..2106)
                     /locus_tag="AC16V7_00290"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR6255585.1"
                     /translation="MRFRLILLVCMILILAACQDSEGSSTGGGGADDPPPLLSTTTVN
                     RQAPTSLPSRKGTTIRSNDLPVETADPTQPQSAPMAPDTEEPAPAFSVCSPLVDTPLE
                     ELSQIVSEPYRPPPPNSEARHHGVDFAYFRRYDRAAIEGAGIQSVLNGQVAASIVDSF
                     PYGNMLILETPGSLLPPELADRISMGAGESLYTLYAHMERPPALRLGDSVRACQPLGN
                     VGKSGNAGGSHLHLETRLGPAGTRFSGMAYYKVWATDEQRENYLLWRTSGTYRHFDPL
                     TLWTSDPK"
     gene            2302..2376
                     /locus_tag="AC16V7_00295"
     tRNA            2302..2376
                     /locus_tag="AC16V7_00295"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2337..2339,aa:Pro,seq:ggg)
BASE COUNT          605 a          666 c          635 g          550 t
ORIGIN      
        1 caacaacaag gatggctcct gactagtcag gtcaagcatc catctctttt ctatttacga
       61 ggtgcaacta tatctcttcc aaaatcaatc ggcttccctc tcatgatgaa ggaacctggt
      121 gaaaaacggg ttttcctccc tgagttcatc caatacctga gcgaatcagg tttaattatt
      181 tttatcgaag agagctacgg ctctcgctca gggtggagtt tcgacgatta caagcagggg
      241 aatgagaacg tgcgctcgtg ttctcaactg gaggccttcc agcaagacat cgttgtggtg
      301 ctgcgttctc cgacttttga ggagtttcag ctgctacgtc ccggcactaa tctgatatcg
      361 atgctgcact ttcccactcg tccaaaacgg gtagaagcgc tcaaagaact tggggtagac
      421 gctatatccc tggacagcat tgtcgatgac aacaacattc gcctagtcga aaacatgcgt
      481 gcggtggctt ggaacggatt ggaggctgcc ttcgatgtgt tagagaatcg ctggcctgga
      541 ttgaggcgac cggacggcca gccaatccgg gtgctaatcc tgggcactgg catggtaggc
      601 aagcatgcca gtgaagccgc cacgaaattg ggcaatattg aacgcaataa ccagcatatt
      661 gaagatggtg gacccggatc ggttgcgatc tccattgggc gcaatatcac gcaccaccct
      721 gagaggctca agaagctatt caacgaagcc gatatattag tggatgccac ccaacgccgc
      781 gacacttctg ttccggtagt gccaaacgcc tggatagaat ggctgccaca acacgccgtg
      841 attacagatc tggcagtgga cccctacacg ctagacaccc aacctccagt ggtccgcggt
      901 atccagggta tcccaaaggg aaacttggac cagtatgtct tctatcccga cgaccctaac
      961 tgggagaaga cagtgcccac cactgtcccc aaccaacacc ggcgtacggt ggtttcctgt
     1021 tactcctggc caggcattca tccagaagcc tgcatgcgtc actatgccct acaacttgat
     1081 cccctaatgg aagtcctgat tacaaaaggg tatgacggcc ttgaccttca cggtggttat
     1141 tttgagcgcg cgatgtatcg tggcacccta agaacatggc tgaatagcgg gcactacgaa
     1201 cctcgcccac gctaaggctt agtacgcact atcaccgtaa aggctcgtct tttattttgg
     1261 atcactcgtc caaagggtca aggggtcaaa atggcgatat gtcccgctag tgcgccataa
     1321 gagataattt tcccgctgct catccgttgc ccaaaccttg taataagcca taccagagaa
     1381 acgcgtcccg gctggtccga gacgagtttc caggtgtagg tgagagccgc cagcattgcc
     1441 gctcttaccc acattaccca gcggctggca ggcgcgtacg ctatccccca acctcagcgc
     1501 aggtggcctc tccatatggg catagagtgt gtaaagcgac tctccagcac ccattgagat
     1561 ccgatccgcc agttctggcg gcagcagcga gccaggcgtc tccaaaatga gcatattccc
     1621 atatgggaag ctatccacaa tcgatgcagc cacctgtcca ttcaacacag attgaatccc
     1681 agctccctca atcgctgccc ggtcgtagcg gcggaaatat gcgaaatcca caccgtgatg
     1741 gcgcgcctcg ctattcgggg gtgggggtcg gtaaggttcc gatacgatct gagataattc
     1801 ttccaggggg gtatctacca gcggtgagca aaccgaaaaa gccggcgctg gttcttctgt
     1861 atcaggtgcc ataggagcgc tttggggttg ggttggatct gcagtctcta ccggaaggtc
     1921 attcgatcgt atggtagtgc ccttgcgaga gggcaggctg gtgggcgctt gtcgattaac
     1981 agtagtcgtg gagagcagtg gcggaggatc atcagctcct ccaccaccgg tagaactgcc
     2041 ttcagaatcc tggcaagccg ccagaatcaa gatcatacac acgaggagaa tgagtcgaaa
     2101 tcgcattaac catcgctctt atgtgaaaca caagatgcta taaatattgt aaccgacatc
     2161 ctttgcatat tcgcccatta cacttactcg cgtagcgaat ctgatagaag aactgcatca
     2221 catcacccct tagctcactc aagcgttgac aatatccccc cagtaaagta gaatacgcct
     2281 cgctccctta accagggtat tcggggcgtg gcgcagaccg gctagcgcgc ttgcatgggg
     2341 tgcaagaggc cctgggttca aatcccagcg ccccgactaa agactgcttg tgtagaactg
     2401 taatctacac aagcggtttg cggttaatag aggggtggaa acggagaccg catatg
//
LOCUS       DBNHOH010000018         2282 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_193042, whole genome shotgun sequence.
ACCESSION   DBNHOH010000018 DBNHOH010000000
VERSION     DBNHOH010000018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_193042"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="AC16V7_00300"
     CDS             complement(<1..1073)
                     /locus_tag="AC16V7_00300"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase
                     (UDP-forming)"
                     /protein_id="HIR6255586.1"
                     /translation="MEPGQNDNKAQQRPIIIASNRGPVSLNRNEAGGYEIQRGSGGLV
                     TALTGLASLIDATWISCALSADDTEWGEGEVPFNDKGDSIFVKFLTPEESAFDGYYNV
                     IANPLLWFLQHSMWDVPRAPVINRATWRAWEEGYEQVNREFALEIANQVNRVSQPVLI
                     MLQDYHLYLAARYLRETVPSQAITILHFIHIPWPGPEYWGILPPTMRQAILEGLCAAD
                     LLGFQTREDALNFMRTCESYQPKASVKYKRGQIWYRNHATHVRDFPISIDVEALKAMA
                     NSPEVQQHHAEIQDIVGGRKLILRIDRIEPSKNIIRGFQAFEEMLELYPEHRDQIIFL
                     ALLVPSRLELEEYKNYLDDLMGTA"
     gene            complement(1101..>2282)
                     /locus_tag="AC16V7_00305"
     CDS             complement(1101..>2282)
                     /locus_tag="AC16V7_00305"
                     /inference="COORDINATES: protein motif:HMM:NF046269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HIR6255587.1"
                     /translation="EKRWQDWFRNVPPVADKYRRAYAYAWWIMANNLINPGGIIHYEA
                     MMPSKASYVGLWLWDSAMHALAYRHIDPEMARNQIRAILACQLPDGMLPDAIYDEGII
                     SEIDHPIKAEVTKPPILAWAALKLHETDPNLEFLQEIYVPLVRWNAWWFSMNDDDVDG
                     LAQYNHPYSSGLDDNPLWDYGMPVESPELNSYLCVQMGSLAIMAELLGMNAEAAMWRR
                     RAEAIVRRMIQDFWDEQEGVFRATHNDQIIPVLTPFNLYPLWTGQLPENYRTRLLDHL
                     TNPDEFWGEYILPSVARSDPHYDPDTMWRGPVWSNINYFFIEALNQIGEKDLADKLRD
                     KTLEMILAHPGMYEYYNSETGQPPARSAEAFGWTAAVFIDLAISASQEQQAGSQQSIV
                     D"
BASE COUNT          573 a          552 c          553 g          604 t
ORIGIN      
        1 gcggtgccca ttaaatcatc caggtaattt ttatattcct ctaactccaa gcgcgagggt
       61 accagcaagg ctagaaaaat aatctgatca cgatgttcgg gatacaattc aagcatctcc
      121 tcaaatgcct gaaagccgcg gatgatgttt ttgctaggtt caatgcgatc aatgcgcagg
      181 atcagtttgc gtccaccaac aatgtcctgt atctcagcgt gatgttgctg aacctcaggt
      241 gaattggcca ttgccttcaa agcctcaacg tcgatggaaa tgggaaaatc gcgcacatga
      301 gtggcatgat tacggtacca gatttgacca cgcttgtatt ttacgctggc ttttggttgg
      361 taagattcac aggtgcgcat gaagtttaag gcatcctcgc gtgtctgaaa gccaagtaaa
      421 tcagctgcac acagcccttc taagattgcc tggcgcatgg ttggtggtaa tattccccag
      481 tactctgggc caggccatgg aatatggata aaatgcaaga tcgttattgc ttggcttggg
      541 acggtctctc gcaggtatcg agctgcgagg tagagatgat aatcttgcaa cataatcaat
      601 actggttgag aaacgcgatt gacctgattg gcaatctcca gggcaaactc tcgattgacc
      661 tgctcataac cttcttccca ggcccgccat gtggcgcggt tgatcaccgg tgcgcgcgga
      721 acatcccaca tggaatgctg tagaaaccag agtaatggat tggcgatcac attgtaatag
      781 ccatcaaagg cggattcttc gggtgtcaga aattttacga atattgaatc gcctttatca
      841 ttaaatggta cttcaccttc tccccattct gtgtcatcag cgctcaaggc gcaggaaatc
      901 caggtggcat cgataaggct agctaaacct gttagagctg tgaccagacc accgctgccg
      961 cgctgaatct cgtaaccccc agcttcattc cggtttaaag ataccggtcc gcggttggat
     1021 gcgataatga tcggacgttg ttgcgccttg ttatcgtttt gacctggttc cataatcaca
     1081 actctccttt accagggcta tcaatcaact atagactgtt gggagccggc ttgttgctcc
     1141 tgactggcgg aaattgctaa gtcgatgaac acagctgctg tccagccgaa agcttcggcg
     1201 gaacgcgcgg gaggctgccc ggtttcggaa ttgtagtatt catacatgcc aggatgagcc
     1261 aggatcattt ccaaagtttt gtccctcagt ttatccgcta aatccttctc accaatttga
     1321 tttaaggctt caataaaaaa atagttgatg ttcgaccata ctggcccacg ccacatcgta
     1381 tcagggtcat aatgcggatc acttcgtgca acggatggga ggatatattc cccccagaat
     1441 tcatcagggt tggtgaggtg atccaatagt cgagtgcggt aattttcggg tagctgcccg
     1501 gtccacaaag ggtaaagatt gaagggtgtc aaaacaggaa tgatttggtc gttgtgcgtt
     1561 gccctaaaga caccttcttg ttcgtcccaa aaatcttgga tcatgcgtcg cacgatcgcc
     1621 tcagcccgcc tgcgccacat cgcagcttcg gcgttcatgc ccagtaattc ggccataatt
     1681 gccaacgagc ccatttggac gcaaaggtag ctattcagtt ctggtgattc tactggcata
     1741 ccataatccc atagtggatt gtcatctaga ccggatgagt aagggtggtt gtactgtgct
     1801 aaaccgtcga catcatcatc attcatgctg aaccaccagg cattccagcg taccaatggt
     1861 acatatatct cttgtaagaa ttcaagatta gggtcagttt cgtgtagttt taaggctgcc
     1921 caagctaaaa tgggaggttt cgtgacttca gccttgattg ggtggtcaat ctcggaaata
     1981 atgccttcat catatattgc atctggcagc atcccatcag gaagctggca agccagaata
     2041 gcacgaatct ggttgcgggc catctcagga tcgatgtgac ggtaggccag cgcgtgcatg
     2101 gcactatccc acagccataa tccaacataa cttgccttag atggcatcat ggcttcataa
     2161 tgtatgatac ctccgggatt gatcaagtta tttgccatga tccaccaggc ataggcgtag
     2221 gctctgcggt atttatcagc caccggtggc acattgcgaa accaatcttg ccaacgtttc
     2281 tc
//
LOCUS       DBNHOH010000019         1764 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_385239, whole genome shotgun sequence.
ACCESSION   DBNHOH010000019 DBNHOH010000000
VERSION     DBNHOH010000019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_385239"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1490)
                     /gene="miaB"
                     /locus_tag="AC16V7_00310"
     CDS             complement(111..1490)
                     /gene="miaB"
                     /locus_tag="AC16V7_00310"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HIR6255588.1"
                     /translation="MKYHIWTEGCQMNVADSMRVASALEHLGYQSVNRADEADVIVLN
                     TCVVRQSAEDKAYGRLHSLRPLKQQHPELVINLMGCMVGIKGYDHLKKRFPFVDVFSP
                     PSDPGPLVAYLTQDESRELEQAETERRFALMDAQPGDDGSLLLPLHERNRLVAAHVPV
                     VLGCSHACTFCIIPYRRGVERSRHVGDIVSEVRSLVDQGVKEVTLLGQIVDRYGKDIP
                     DGPDLASLLRITHQVEGLERIRFLTSHPNWMTDELLDTVAELPKVMPHIEVPVQAGDD
                     QVLENMKRGYTADDYRRLIQKIRERIPLVSIATDIIVGFPGESEAQFQRTYDLLAELK
                     MDVAHLARYSTRPGTVAERRMPDDVPDQEKWRRFRTLEELQENIATEINAGYLGEVVE
                     VLFEEKVRGRWKGRTPTNKLVFVESKADLHGQVLPVEITWTGPWSMQARLKTQRGAEI
                     PLDLVSISI"
BASE COUNT          399 a          474 c          479 g          412 t
ORIGIN      
        1 cccgaagccg caggcggtgg gagggggaac gagggggtgg gaaaatcggc tgcgaaacat
       61 attaaattta gggtgtttta tgtcttccga aagagcctaa gtttattgtt ttatatggag
      121 atcgatacca gatcgagtgg aatctccgcc ccacgctggg ttttcagcct ggcctgcata
      181 gaccatgggc cagtccaggt gatttctact ggaagcacct gaccgtgcaa gtctgcttta
      241 ctctcgacaa agaccaattt gttggtgggt gtgcgccctt tccagcgacc acgcaccttc
      301 tcttcgaata acacttcaac tacctcgccc agatagccgg cgttgatctc ggtggcgatg
      361 ttctcctgta gctcctccag ggtgcggaat cgtcgccatt tctcctggtc gggcacgtcg
      421 tcgggcatgc gccgctcggc gactgtcccc gggcgcgtgg agtaacgcgc taggtgagcc
      481 acatccatct taagttctgc cagcaagtca taagtgcgct ggaattgggc ttcgctttcg
      541 ccggggaagc cgacgatgat atcagtggcg atggagacaa gcgggatgcg ctcccggatc
      601 ttttggatca gtcggcggta gtcatcggcg gtgtagccgc gtttcatgtt ttcgagtacc
      661 tggtcatcgc ctgcctgcac aggcacctcg atatgcggca tgacttttgg cagctccgct
      721 accgtgtcca gcagctcgtc agtcatccag ttggggtgag aggtcaagaa gcggatgcgc
      781 tccagacctt ccacttggtg ggtgatgcgc agtagggagg ccaggtctgg accgtccggg
      841 atatctttgc cgtagcggtc aacaatctgc ccaagcaggg tgacctcttt gaccccctga
      901 tcgaccaggg agcgcacttc actgacgata tcacccacat ggcggctgcg ttccaccccg
      961 cgacgatagg ggataatgca gaacgtgcag gcatgcgagc aacccaatac caccgggaca
     1021 tgcgctgcca ccagccggtt gcgctcatga aggggaagga gcaagctgcc gtcgtcgccg
     1081 ggttgcgcgt ccatcagggc gaaacgccgt tcagtctccg cttgttccag ctcgcggctt
     1141 tcatcttgtg tcaggtaggc cacaagcggg ccaggatcgg atggtggaga gaacacgtct
     1201 acaaacggaa aacgtttctt tagatgatca tatcctttta tccccaccat acatcccatc
     1261 aagttgatca ctagttctgg gtgttgctgt ttgagcgggc gtaatgaatg cagacgcccg
     1321 taggctttat cttcggcgct ctgcctaacc acgcaagtat tcagcacgat cacgtccgct
     1381 tcatcggcgc gattcacaga ctggtagcct aggtgttcga gcgctgatgc cacgcgcatc
     1441 gaatcggcca cgttcatctg gcagccctca gtccaaatgt gatatttcat gtccgttatt
     1501 ataacaaaag agatagggga atggtaaatt gcgattaatg aatgcagggc aattgcatat
     1561 gccctgaggg gcataccccc accaaaacac cccattttct ctcctcagtg gtggaaaatc
     1621 gcccaaatgc gattgccctg ttaatgaatg taaggcagga gacagtatca agttataatg
     1681 atactcaata agtcagaaaa atccgtatat cattttatat ttactatgca acccaaaaag
     1741 accccccaac ccaaaaagat atat
//
LOCUS       DBNHOH010000020         1805 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1538947, whole genome shotgun sequence.
ACCESSION   DBNHOH010000020 DBNHOH010000000
VERSION     DBNHOH010000020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1538947"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="AC16V7_00315"
     CDS             complement(<1..961)
                     /locus_tag="AC16V7_00315"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR6255589.1"
                     /translation="MALSNYTSIYFTPLEDELRHAVAIVNGDGLSELYEMLAYHMGWQ
                     GEGTGIGTSGKRVRPLLLLLSCEAAGGNWKDALPAAAAVELVHNFSLIHDDIEDNSPL
                     RRGRPTVWKKWGIPQALNAGDSLFTLAHLSILRLEEKVSPAITINAIRILHETCLKLT
                     QGQYMDISYEDRKDLTLEAYWPMVSGKTAALLAACTGLGALIAGADDERQDNFCQFGH
                     YLGLAFQAQDDLLGVWGNAALTGKSAESDLLTGKKSLPVLFGLSLNGPFAQRWKQGSL
                     HPDEIPLLANQLEAEGARRYTQETVDTLTHQALSHLHKAVSGNE"
     gene            complement(1028..>1805)
                     /gene="fni"
                     /locus_tag="AC16V7_00320"
     CDS             complement(1028..>1805)
                     /gene="fni"
                     /locus_tag="AC16V7_00320"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HIR6255590.1"
                     /translation="AAQETGVAMGLGSQRAAIEQPDLAVTFKVRDVAPDILLLANLGA
                     VQLNYRYGIAECQRAVDMIEADALILHLNALQEAVQPEGDTRFAGLLKKIEHVCKSLP
                     VPVIAKEVGWGFSEKAAKMLAEAGIAAIDVAGAGGTSWSQVEMHRAQDESQSRLAAAF
                     INWGIPTAESILNVRRAAPGLPIFASGGLRSGVEIAKSIALGAHLGGMAGPFLKAAVR
                     SLEDTLQSIAEIRNEIKVCMFAAGVPDLESLRNTQLVKID"
BASE COUNT          427 a          479 c          458 g          441 t
ORIGIN      
        1 cttcgtttcc agacacagct ttgtgtagat gactcaaagc ctggtgggtg agcgtgtcaa
       61 cagtttcttg agtatagcga cgggcgcctt cggcttctaa ctgatttgcc aggaggggga
      121 tttcatcggg atgcaatgac ccttgcttcc agcgctgcgc aaagggaccg tttaggctga
      181 ggccaaagag cacgggaaga gattttttac cagtaagcag atcgctctca gcagatttcc
      241 ctgtcagagc tgcattaccc cacacaccca gcaaatcatc ttgagcttgg aatgccaatc
      301 ccaagtaatg accaaattgg caaaaattat cctgcctctc atcatctgct cctgcaatga
      361 gcgcgcccaa cccggtgcaa gcggccagta acgcagcggt tttaccgctc accattggcc
      421 agtatgcttc taaggttagg tctttccgat cttcgtagga gatatccata tattgcccct
      481 gggtgagctt gaggcatgtt tcatgtaaaa tgcggattgc attgatggtg atggcagggg
      541 acactttctc ttccaatcgc agaatcgaca agtgcgccaa ggtgaagagc gaatcgccgg
      601 cgttaagagc ctgcggtatg ccccatttct tccagacagt agggcggccg cgtcgtaatg
      661 ggctgttatc ttcaatatcg tcgtggatga gcgagaagtt gtgcactagc tctacagcag
      721 ccgcagccgg aagggcgtct ttccaatttc cgccagcagc ttcgcacgat aacagcaaca
      781 gtaatgggcg aaccctttta cccgatgttc caataccagt gccctcccct tgccagccca
      841 tgtggtaggc gagcatttca tacagctctg acaacccgtc gccattcaca atcgctacgg
      901 catggcgaag ttcgtcttct aagggcgtaa aatatatcga agtgtaattt gacagcgcca
      961 tagtattcct agaataaaaa atctttttgt tccactccgt tccgaaaagg gtacttcggg
     1021 taaaatgtca atcgattttg acaagctgag tattgcgcaa gctttctaga tctggcactc
     1081 ctgctgcaaa catacacacc ttgatttcat ttcggatttc agcgatgctc tggagtgtat
     1141 cttccaggga acgaacagcg gctttgagga agggtccagc catgccacct aagtgggctc
     1201 caagggctat ggatttggcg atttctaccc cgctgcgcag gccacccgag gcaaaaattg
     1261 gcaaaccagg tgcagcgcgc cgtacattaa gaattgattc ggcggtaggt atgccccaat
     1321 ttatgaatgc tgctgccagt ctggattgac tctcgtcttg ggcgcggtgc atctcaacct
     1381 gcgaccagga tgtgccacca gccccggcaa catcgatggc agcaatgcca gcctcggcta
     1441 gcattttggc agctttctca gagaatcccc aaccgacctc tttggcgatg acgggcacag
     1501 ggagggattt acacacatgt tcaatttttt tcaagagccc agcaaagcgc gtgtctcctt
     1561 cgggctggac agcctcttga agggcattta ggtgcaaaat caatgcatct gcctcgatca
     1621 tatccacagc acgctgacat tcggctatcc cataacggta gttaagttgt acggctccaa
     1681 gattagcgag aagcaaaata tcgggggcga cgtcccgtac tttgaaggtg acggctaaat
     1741 ccggttgctc aatagccgct ctttgggagc ctagccccat ggccacgcct gtctcctggg
     1801 ccgct
//
LOCUS       DBNHOH010000021         3964 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1090331, whole genome shotgun sequence.
ACCESSION   DBNHOH010000021 DBNHOH010000000
VERSION     DBNHOH010000021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1090331"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="AC16V7_00325"
     CDS             <1..304
                     /locus_tag="AC16V7_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255591.1"
                     /translation="RLDGTEGVLDYQTDMRIWPTADQMDAATILTYRTKSAVEQLKFE
                     PRDMLVDELDEFARCIQVSAQPETGALEGLRALDVILSSIRSHETGTPIALGEYNA"
     gene            297..1355
                     /locus_tag="AC16V7_00330"
     CDS             297..1355
                     /locus_tag="AC16V7_00330"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR6255592.1"
                     /translation="MPDERVEAAIKNWAPRFTSQGVDYNDFFRTTARIQKWDEWCREW
                     CVTGDVHHDLAVKAEAQGNSLSAGEAYISAALCYHFGKFVFQDFHDEYMSASQKAVEA
                     FAKGLKLLDPTGERLEIPLDKIRMVGTLRRPPESDKPPLVILLPGLDSTKEEFFHWER
                     VFLKRGMATFTLEGPGQGECGYNSHIRPDYEVAVSAALDILTKRSEIDPNRIGLAGVS
                     LGGYYAPRAAAFEPRLKAAVGNCGPWNFGECWDKLPSLTRSAFLYHSGASDMEDAKAR
                     AHQLSLDGIAQKIKQPLLVIHGKLDRLIPWQHAHQIVDAVGPNAQLAMFENGNHVCNN
                     IPYIYRPLTADWLKEKLG"
     gene            1352..2554
                     /locus_tag="AC16V7_00335"
     CDS             1352..2554
                     /locus_tag="AC16V7_00335"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR6255593.1"
                     /translation="MTYPRFSDAEFQRRYRLARELMRREGLSALVVFGNSGINRHNEA
                     NVFWLTNYLDLHHNYLVFPLHGKPTLLTGLVNHVPNARAIAVINDTRWGTYDPAKEIA
                     AQLVKTGIGKDHVGLVGVNATFGMGMPYRHYATLKEALPETEWVDVTREFARLRMVKS
                     EEEFKWLKKAAEFTDMTIEALESEVKPGLTEHDLIGIIEGSYRRKGGQPHIAFLRSMP
                     MGAPTACVPAQNVTERVIQKGDIIIMEISASYWGYSGQIHRPIAVGRDPTPEWQKLFD
                     IGVQAYDRMCEAIRPGAIEGDVIKAASIIGESGYRIYDDLIHGYGVDIHPPLIDSSCC
                     DYWPWNDSHPAPAGRTFAEDMAIVVQPNPITPDERMGLQVGALTRVTKDGAVSLQEYP
                     MKFIVAKG"
     gene            2557..2736
                     /locus_tag="AC16V7_00340"
     CDS             2557..2736
                     /locus_tag="AC16V7_00340"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR6255594.1"
                     /translation="MGQPDYLRTIRARGVWLGGRVNDLFIRDFPVLRSDEPPHNEGTD
                     TGPTPLEITLAGLCA"
     gene            2758..3012
                     /locus_tag="AC16V7_00345"
     CDS             2758..3012
                     /locus_tag="AC16V7_00345"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR6255595.1"
                     /translation="MAEKIRFDLQGLEASAEGVLDIRGRKGEANVPVHFSSVRLHVKI
                     KTSESDERIQRLIELAEKYCPVQSLIRAAVPDFEVTWEHL"
     gene            3009..>3964
                     /locus_tag="AC16V7_00350"
     CDS             3009..>3964
                     /locus_tag="AC16V7_00350"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6255596.1"
                     /translation="MSLKTKVLYLVSLAAFLRSFAQVIYVPSLATMRTELNTTTAMIG
                     LTLSVYGLCLAFAQIAYGPIVDRFDSKRILLIGLVIYLLGNLSAYFTRGIGLLLVARS
                     MQALGIAAAAGVGIALITDVFPPAQRGRAMGIFSMFNSAGAASGPAVGGLIAVWLTWR
                     ADFLVLAIVTIALLFFTTWKLPAQPVHGQKVGLKDMLTIMQTRPTFGALTLGLVQFYG
                     LYTIHTLTPILLTEKLGMHEAGIGAVTTSTAIGVIIGTFVGGRAADKLGARFTLVVGA
                     MGTSVAFTVLTIISSIASTGMSPLVVSAGLLLFGLTAGYGFPA"
BASE COUNT          870 a         1023 c         1125 g          946 t
ORIGIN      
        1 gcgccttgac gggacggagg gagtgctcga ttaccaaacg gatatgagaa tctggccaac
       61 tgccgaccag atggatgcag ccaccatttt gacttacagg accaaatctg ctgtagagca
      121 gctgaaattt gagccgcgtg acatgctggt ggatgaattg gatgaatttg cccgttgcat
      181 ccaggtttca gcccagcctg agacgggcgc attggaagga ctgcgagcgc tggatgtaat
      241 tttgtcatct atccgctctc atgaaacagg aacccctatt gcactaggag aatacaatgc
      301 ctgatgaacg agttgaagct gcgataaaaa actgggctcc gcgctttacg tcccagggcg
      361 tggattataa cgactttttc cgcaccaccg cccgcatcca aaaatgggat gaatggtgcc
      421 gcgagtggtg cgtgacaggt gatgtgcatc atgacctcgc cgtaaaagct gaagcgcagg
      481 gtaattctct cagcgcaggc gaggcctata tctctgcggc gctttgttac cattttggca
      541 agttcgtctt tcaggatttc catgatgaat atatgtccgc ttcgcaaaaa gcggtggagg
      601 ctttcgccaa aggcctgaaa ttgctcgacc caacagggga acgtttggag atcccgttag
      661 acaaaataag gatggtaggg accttgcgcc gacctccaga atcggataag ccaccactcg
      721 tgattctctt gcccgggctt gattccacca aagaagaatt cttccactgg gagcgggtct
      781 tcctgaaacg cggcatggcg accttcaccc tggaaggtcc tggacagggc gagtgcggct
      841 acaactcgca tatccgccct gattacgagg tggctgtctc ggctgcgctg gatatattga
      901 caaagcgaag cgaaatcgat cccaaccgca tcgggctggc gggagtcagc ttgggtggat
      961 attacgctcc gcgcgcggca gcctttgagc cgcgcctgaa agctgcggta ggcaattgtg
     1021 gtccctggaa ctttggggaa tgttgggata agctgccatc gctcacacgg tctgcctttc
     1081 tgtatcactc aggagcgagt gacatggaag atgcaaaggc ccgcgcacat cagctatccc
     1141 tggatgggat tgcgcaaaag atcaaacaac cgctattggt catccatggc aaacttgatc
     1201 gtttgatccc ctggcagcat gctcaccaga tcgtggatgc tgtgggccct aacgcgcagc
     1261 tggctatgtt cgagaacggg aatcacgttt gcaataatat cccctatatt taccgcccat
     1321 tgaccgctga ctggctcaag gagaagcttg gatgacttat ccccgcttct cggatgcgga
     1381 gttccagagg cgataccgtc ttgcccgcga gctgatgcgc cgtgagggtc tatctgctct
     1441 ggtggtgttt ggaaactctg gcatcaaccg tcataatgag gccaatgtct tttggttgac
     1501 aaattacctc gacctgcacc acaactatct ggtctttccg ctgcatggta agccaactct
     1561 tctcacgggt ctggtgaacc atgtgcccaa tgcgcgcgcg attgccgtga ttaacgacac
     1621 gcgctgggga acgtatgacc ccgcaaaaga gatcgctgcg cagctggtta agacaggcat
     1681 cggtaaagat catgtcgggt tggtaggcgt caacgcgacc tttgggatgg ggatgcctta
     1741 tcgacattac gcgactttga aagaagcgct ccccgaaacc gaatgggttg acgtgacccg
     1801 cgaatttgcc cgcctgcgca tggtcaaaag cgaagaagaa tttaaatggc tcaagaaagc
     1861 ggccgagttc acagacatga ccatcgaggc gctggagtcc gaggtgaagc cagggcttac
     1921 cgagcatgat ttgataggta ttattgaagg atcttatcgc cgtaagggcg ggcaaccaca
     1981 tatcgctttc ttgcgttcta tgcccatggg tgcaccaacc gcgtgcgtgc ccgcgcaaaa
     2041 cgtcaccgag cgcgtcattc agaaaggcga tatcatcatc atggagatca gcgccagcta
     2101 ctggggctat tctgggcaga ttcaccgtcc aattgcagta gggcgcgatc caacacccga
     2161 gtggcagaaa ttgttcgata tcggtgtgca ggcttatgac cgaatgtgtg aggcgatccg
     2221 tccaggcgca atagaagggg atgtgatcaa agcggcctca atcatcgggg agtctggtta
     2281 tcgtatctac gatgacctga tccatggcta tggcgtggac atccatccac cattgatcga
     2341 tagttcgtgt tgtgattact ggccctggaa tgactcgcat cctgcgccag ctggcaggac
     2401 ttttgccgaa gatatggcga tcgtggttca acccaatccc atcacgcctg atgaacgtat
     2461 gggcttgcag gtgggtgcct tgacgcgcgt gaccaaagac ggcgcggtca gtttacagga
     2521 gtatccgatg aagtttattg tggcgaaagg gtagtgatgg gacaaccaga ttatctgcgc
     2581 acgatccgtg ctcgaggggt ctggcttggt ggacgggtga atgatctttt catccgcgat
     2641 ttccctgtct tgcgttcaga tgagccaccg cacaacgagg gcacggatac cggtcccacc
     2701 ccgctggaga tcacgcttgc aggcctgtgt gcctgaacgg gtgtctcaac ggccaggatg
     2761 gccgaaaaga tccgttttga cttgcaggga ctggaagctt ccgccgaggg tgtgctcgac
     2821 attcgcgggc gcaagggcga agctaatgtg cctgtacatt tcagcagcgt ccgtttgcac
     2881 gtcaagatca agacaagtga atcggatgaa cgcatacagc gtttgatcga attggctgag
     2941 aaatactgcc ctgtgcaaag cctgattcgt gctgctgtgc cagactttga agttacctgg
     3001 gagcatttat gagtctaaaa acgaaggtcc tttatctggt ttcgttggca gccttcctga
     3061 gatcctttgc gcaggtgatc tacgttccct cgctggctac catgcgcacc gagctgaata
     3121 cgaccacagc tatgatcggt ctgacactct ctgtttacgg tttgtgcctg gcgttcgcgc
     3181 agatcgccta tggcccgatc gtggaccgtt tcgacagcaa acgcatattg ctgattggcc
     3241 tggtaattta cttgctcggc aatcttagcg catacttcac acgaggaatt ggtttgctgc
     3301 ttgtggcgcg cagtatgcaa gcgttgggta tcgctgcggc ggctggcgtt ggtattgcgc
     3361 tgatcacgga tgtatttcct ccagcacaac gtggtcgcgc catgggcata ttttccatgt
     3421 ttaactcggc aggtgctgcc tctggccctg cagtcggtgg actgattgcg gtctggttga
     3481 cttggcgagc cgactttcta gtccttgcga ttgtgacgat cgccctgctg ttcttcacta
     3541 cctggaagtt gcccgcgcaa cccgtacacg ggcagaaagt tggcttgaag gacatgttga
     3601 ccattatgca gacgcgcccc acttttggcg ccctcaccct gggcctggtg cagttctatg
     3661 ggctttacac gattcataca ctgaccccaa tcctgcttac cgagaaattg ggcatgcacg
     3721 aagccggtat cggtgcggtg acaacctcca cagctatcgg cgtgatcatc ggtacatttg
     3781 tgggcggtcg agccgcagat aagctcggcg ctcgctttac cttagtagta ggggcaatgg
     3841 ggacctcggt tgcatttacc gtcctcacga tcatcagttc aatcgcctca accggcatgt
     3901 cgccactggt cgtatcggca ggattattgc tatttgggtt aaccgcaggc tatggatttc
     3961 ctgc
//
LOCUS       DBNHOH010000022         3462 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1410955, whole genome shotgun sequence.
ACCESSION   DBNHOH010000022 DBNHOH010000000
VERSION     DBNHOH010000022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1410955"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..1044)
                     /locus_tag="AC16V7_00355"
     CDS             complement(112..1044)
                     /locus_tag="AC16V7_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR6255597.1"
                     /translation="MSVHIAVDIGGTHMRAAYYPAHSHEPLEHKKITTKHPSESAWER
                     LVKLITSIWPAGEEVASIGVAAPGPLDPFSGVLFSAPNIHEWINLPLRKLLEDQFEVP
                     IAVGNDANLAALGEWKFGAGIGHKHLVYLTISTGIGGGVIIDNRLLVGTRGLTAEFGH
                     VSVQPDGPLCGCGQRGHLEALAAGPAIARWVETEIAQGVPSSLAKLAQFTTKQVAEAA
                     NQGDDLAIRALNRAGNFIGRALADILHIFNPSIIIIGGGVSRSGDLLMHPIRKEMEAR
                     VMNPHYLDNLTLTTAALGDETGLLGALALARDIK"
     gene            1117..2451
                     /locus_tag="AC16V7_00360"
     CDS             1117..2451
                     /locus_tag="AC16V7_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016869756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HIR6255598.1"
                     /translation="MEKDSAPLYRRWLNAAARSLRWLVPGLGVKRWLLVILAGTTLIG
                     VGLAIIILDIYRTTPDTWWLPLLSTASLRFIARPLRAIIFGGLGLGLIITGIYGLNRT
                     ILRPFLRPGRHVVDAITQHRRKERGPRVVAIGGGNGLSTLLRGIKSYTHNITAIVSVA
                     DDGGSSGRIRREIGILPPGDIRNCLAALSSDEALLTQLFQYRFPEGNEDLVGHSFGNL
                     FISALADLTGSFEDAVAESGRVLAVNGRVVPSTLHDVRLEADLKLPHLVSEVRIKGES
                     HIPEASGSIRRVWLEPNNPPAFPQAVRAILAADLIIVGPGSLYTSILPNLLVPDILEA
                     IRASRALRIYVCNVATQPGETQNYTCGDHIRAIEEHVGDGLFEVIVANNNYESDLPGP
                     SSWVRVEDDLDVDHAVYRADLIANDFPWRHDSDKLATVLMDILAERTGPLVE"
     gene            2563..>3462
                     /gene="gap"
                     /locus_tag="AC16V7_00365"
     CDS             2563..>3462
                     /gene="gap"
                     /locus_tag="AC16V7_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR6255599.1"
                     /translation="MKVKVGINGFGRIGRQVLKAIGDYHPDSLEVVAFNDIGDLKTMA
                     HLLKYDSNYGRFDGEIELGKDELIINGKSVKVFKETDPAKIPWGDLGVEIVIESTGIF
                     TIKKDGVNKKGKQVQGAENHITKGGAKKVIISAPAEGEDLTVVLGVNDDKYDPAVHHV
                     ISNASCTTNCLAPAAKVVNDKFKIKRAFMTTIHAYTNDQRILDLPHSDLRRARAAAMN
                     IIPTTTGAARALKLVIPELAGKFDGYALRVPTSTVSVVDFTADVEIATTTEELRQAFR
                     DAAAGPMKGILAAVEEPLVSVDFK"
BASE COUNT          839 a          851 c          926 g          846 t
ORIGIN      
        1 gggttgcgta accgcgattg tataggattt gccgcaatat aggcggataa ccatgcaatg
       61 catgatcgac ttccaatggt agtcgtggag ggattatcca gcgtttttcc attatttaat
      121 atcccgcgca agagctaatg ctcctagtag ccccgtctca tctcctaggg cggcagtggt
      181 cagggtcagg ttgtcaagat aatgcgggtt cattacgcgc gcttccattt cctttcgaat
      241 cgggtgcatg agaaggtcgc cactgcgcga aacgccacca ccgataataa tgatagatgg
      301 gttaaagata tgcagtatgt ctgccagcgc ccgcccaatg aagttgcctg cgcggttcag
      361 ggcgcgaatg gccaggtcgt ctccttggtt agcggcttca gcaacctgtt tggttgtgaa
      421 ttgcgcaagc tttgccagac tagatggtac accctgagca atttctgtct ccacccaacg
      481 agcaatggcg ggtcccgcag ccagtgcttc caagtggccg cgctgaccgc atccgcaaag
      541 tggcccatcg ggttggacac tgacatgtcc aaattcggcg gttaggccgc gcgttcctac
      601 taacaggcga ttatcaatga taaccccacc tcctatccct gtgctgattg tcaaatatac
      661 aaggtgttta tgacctatcc ctgcaccaaa cttccattcg ccaagggcgg caaggttggc
      721 atcattaccc actgcaatag gaacttcaaa ttgatcctca agcaacttgc gtaagggcag
      781 attgatccat tcatggatat tcggagcaga gaataaaact ccgctaaatg ggtccaatgg
      841 accgggtgct gccacgccaa tgctagccac ttcctctccc gcgggccaga tggaggtgat
      901 caatttcacc aggcgctccc aggccgactc gctgggatgc ttggtagtga tcttcttgtg
      961 ttcgaggggc tcgtggctgt gagcgggata atatgcagcc cgcatatgtg tgcccccaat
     1021 atccacggcg atatgcacac tcatattcga aagtatagca taaagcttgc cccactgttg
     1081 acgagttagg aaaacactgc tatcatcctt gatgatatgg agaaagatag tgctcctctc
     1141 taccgccgct ggctcaacgc agcggcgcga agtttgcggt ggcttgttcc cggattgggg
     1201 gtcaaacgct ggttgctggt aatactggca ggaacgacgt taattggcgt aggcctggca
     1261 atcatcattc tggatattta ccggacaacc cccgatacgt ggtggttgcc actgttatcg
     1321 acggcttcat tgcgatttat cgctcgaccg ctgcgggcga taatttttgg tgggttgggg
     1381 ttggggctta ttatcaccgg tatctatggc ctcaatcgca ctatcctgcg acccttccta
     1441 agacctggac ggcatgttgt tgatgcgatc acccagcatc ggcgcaagga gcgcggcccg
     1501 cgggttgtcg ctattggggg tgggaatggc ctttccacat tgcttcgggg catcaagagc
     1561 tacacgcata atatcaccgc gatcgtctct gtagctgacg atggtggttc atcgggacgt
     1621 atccggcggg agattggcat tctccctccc ggcgatatcc gtaactgcct agctgcactc
     1681 tcaagcgacg aagcgttgct cacccaactc ttccaatacc gctttcctga gggtaatgag
     1741 gaccttgtag gtcattcctt cggtaacctg tttatcagtg ctctggctga tctcaccgga
     1801 agctttgaag acgcggtagc agaatcaggg cgtgttctgg cagtgaatgg tcgggtggtt
     1861 ccctctacgt tgcacgatgt gcgcttggag gccgatttaa aattaccgca tctggtgagc
     1921 gaagtgcgca taaagggaga aagccacatt ccagaagcat cgggcagcat tcgccgcgtc
     1981 tggctggaac caaataaccc gccggctttc cctcaagcgg tgcgtgcgat tttagctgct
     2041 gacctgatta tcgttggacc tggcagccta tataccagca ttttgccaaa tttgctcgtg
     2101 ccagacatac ttgaggcaat acgtgccagc cgggctttaa ggatatatgt gtgtaatgtc
     2161 gccactcagc ctggtgaaac gcaaaattat acgtgtggcg accatattcg tgccattgag
     2221 gaacatgtcg gggatggctt gttcgaggtc attgtggcga ataataatta tgaaagtgat
     2281 ttaccaggac catcgagctg ggtgcgggtg gaagatgatc tggatgttga ccatgctgtc
     2341 taccgtgcgg atctgatagc caatgatttc ccatggcggc atgattcgga taagctcgcc
     2401 acagtattga tggatatatt agctgagcgg actggtccgc tagtagaata agacgtatgt
     2461 atctgggata aattgaaatc tatttattaa tttgatgatt cggtggtcga aacccaccaa
     2521 ttttatacac aacaagatga aactttacga tcggaggcaa aaatgaaagt caaagtgggt
     2581 attaacggat ttggccgtat tggacggcag gttctcaagg caatcggcga ttaccatcct
     2641 gattcacttg aggtcgtggc tttcaacgac atcggtgatt tgaagacgat ggcacactta
     2701 ctcaaatacg actcgaacta tggccgattt gatggtgaga tcgaactcgg aaaagatgag
     2761 ttgatcatca acggaaaatc cgttaaagtc tttaaggaaa ccgatccggc gaaaattccg
     2821 tggggggatt tgggggtaga aatagtcatc gaaagcacag gaatcttcac gattaagaaa
     2881 gatggcgtca ataagaaggg gaaacaggtc cagggagccg aaaatcacat cactaaaggg
     2941 ggagccaaaa aagtgatcat ctccgcacct gcggaaggcg aggatctgac cgtggtttta
     3001 ggtgtcaatg acgacaagta tgaccccgca gtccatcacg taatatcaaa cgcatcctgc
     3061 acgacaaact gccttgcccc ggccgcaaag gtggttaacg acaaattcaa aatcaagcgc
     3121 gccttcatga ccaccattca tgcctatacc aatgaccagc gcatcctaga tctgcctcac
     3181 agcgacctac ggcgcgcccg tgctgcagcc atgaacatta ttcccacgac tactggtgca
     3241 gccagggcac taaagctggt gatccctgaa ttggcaggta aattcgatgg ttatgcgtta
     3301 cgtgtcccaa cctccacagt atcggttgtg gatttcactg ccgatgtcga aattgcgaca
     3361 accactgaag aactgcgaca ggcgttccgc gatgcggctg cgggtccgat gaaaggcatc
     3421 ctggctgctg ttgaggagcc acttgtgagt gttgatttca aa
//
LOCUS       DBNHOH010000023         3577 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_65263, whole genome shotgun sequence.
ACCESSION   DBNHOH010000023 DBNHOH010000000
VERSION     DBNHOH010000023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_65263"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="AC16V7_00370"
     CDS             <1..644
                     /locus_tag="AC16V7_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016641229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HIR6255600.1"
                     /translation="AEVVLVDQLPDSKPGYVSGGDLELVEAAAQRIVRERGAFRADQF
                     NLEGNFRAHFLHTGPEILSQSAGNIDAFCDFAGTAGSFAGCAAAFKEFNPEIKCYLVE
                     PEGAAVLAGKPVTDPNHPIQGGGYAIRDLKLVHNEHVDGYIQVSGGEAIEAARRLAKL
                     EGIFAGFSSGANLAAAIQLLQGDLRGKTVVILINDSGLKYLSTELWEEFNQSG"
     gene            complement(650..1312)
                     /gene="nfi"
                     /locus_tag="AC16V7_00375"
     CDS             complement(650..1312)
                     /gene="nfi"
                     /locus_tag="AC16V7_00375"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034411.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HIR6255601.1"
                     /translation="MAQSHAWDVSPEQAIAIQERLSQQVFRRDQLGPLQVVAGVDAGF
                     ESRGSITRAAVVVLSFPHLQVVDNAVASIPTSFPYVPGLLSFREAPALLQALSKLEKM
                     PDLLLCDGQGIAHPRRLGLASHLGLLLDIPSIGVAKSIFVGQHDPLIDEAGAWQPLLD
                     RGEVVGAAVRTRPGVKPVYVSIGHRLSLETAIELVLRCTGKYRLPEPIRQAHHLASQK
                     GT"
     gene            complement(1378..2073)
                     /locus_tag="AC16V7_00380"
     CDS             complement(1378..2073)
                     /locus_tag="AC16V7_00380"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6255602.1"
                     /translation="MKNTLIRSVIFDFDGLIMDTEYPIFQSWQELFHSQGGRLETSDW
                     VHYVGTMDNERDFVEELEVQIGRKLDRKKLNDARWQRETELIAAQPLLPGVLSYLDDA
                     RRLGLKIGLASSSPWEWVGGHLTQRGLIDYFDCIRTRENVTRLKPEPELYYSVLAALE
                     LTPDQAIVLEDSSPGVTAARRAGLFCVAVPNQLTRLLPLDHANLLLKSLEDLSLEALL
                     EKAAEARNGHGHL"
     gene            2214..2780
                     /locus_tag="AC16V7_00385"
     CDS             2214..2780
                     /locus_tag="AC16V7_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_function: GO:0072570 - ADP-D-ribose binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HIR6255603.1"
                     /translation="MDQVLKEMALPGDCLLQIVQGDITQERVDAIVNAANAQLQHGGG
                     VAGVILRRGGEEIQAESDDWVRQHGAVIHAEPAYTTAGRLSCRYVIHAVGPVWGSGDE
                     DAKLAAAIAGSLRLADQLELASIAFPAISTGIFGFPKKRAACVILNEIRIYFENQRDS
                     GLKQVRLTLFDQETTDAFLEEAEGQSWG"
     gene            2777..3562
                     /locus_tag="AC16V7_00390"
     CDS             2777..3562
                     /locus_tag="AC16V7_00390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012797.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR6255604.1"
                     /translation="MITSVHNPKIKWVRSLQSDARTRREEAAFAVEGVRLADEALAAG
                     WQARLVLFSADLVARGKSIVDGFAAQGAPVEEVAEHVMRAASDTQTPQGILVVLAMRS
                     LPLPHAPGLAFIPDAIRDPGNLGSMLRTAAAAGVEAVLIPPGNVDVYAPKVVRAAMGA
                     HFRLPIVSLSWDEITAYVGMAGLQLVLADAAGGEMHTRSDLRHPLALILGGEAEGASQ
                     EARRLAQARLHIPMAGGVESLNAAAAAAVLLFEVVRQRTQAGK"
BASE COUNT          726 a          973 c         1129 g          749 t
ORIGIN      
        1 gtgctgaagt ggtgctggtg gaccagttgc cagactccaa gcctggctac gtctcaggtg
       61 gcgacctgga gcttgtggag gcagcagccc aacgcatcgt gcgggagagg ggcgcctttc
      121 gagccgatca gttcaacctg gagggcaatt tccgcgccca tttcctgcac accgggccgg
      181 agatactcag ccaatcggcg ggtaatattg atgctttttg tgatttcgct ggcacggccg
      241 gttcttttgc cggatgtgca gcagcattca aggaattcaa ccctgagatt aagtgctacc
      301 tggttgagcc tgagggcgca gcggtgctgg ctggcaagcc agttactgac ccaaaccatc
      361 ccatccaggg cgggggctat gcaatacggg atttgaaact tgtgcataat gagcacgtgg
      421 acggatatat ccaggtcagt ggtggcgaag cgatcgaggc ggcgcgcagg ctggctaagc
      481 tggagggcat ttttgccggg ttctcgtctg gtgctaacct ggctgcagcc atacaactgc
      541 ttcagggtga tttgcggggg aaaacggtgg tgatcttaat caacgattcg gggctgaagt
      601 atttgagcac ggagttgtgg gaggaattca accaatccgg ttaggtaggc tatgtccctt
      661 tctgtgaggc caagtggtgc gcctggcgga taggttcggg caggcgatat ttacctgtgc
      721 agcgcagaac cagctcaata gccgtctcca ggctgaggcg gtgcccgatc gagacgtaaa
      781 ctggcttcac tccggggcgg gtgcgcaccg ccgccccgac cacttcgcca cgatcgagta
      841 ggggttgcca ggcgccggcc tcgtcgatta gcggatcgtg ctgcccgaca aatattgatt
      901 tggccacgcc gatactgggt atgtccagca ataagccaag atggctggcg aggcctaggc
      961 gccgcgggtg ggcgatacct tgaccgtcgc acagcagcag gtcaggcatc ttctctagct
     1021 tggagagagc ctgtaaaaga gcgggcgctt ctctaaagga gagcaagccg ggcacatagg
     1081 ggaaactagt ggggatcgaa gccacagcgt tatccaccac ttgcaaatgc gggaaggaca
     1141 gcaccaccac ggcagcgcgt gtgatcgatc ccctagattc aaagccagca tccacgccag
     1201 ccacgacctg taatggccca agttgatccc ggcgaaaaac ctgctggctc aggcgctctt
     1261 ggatggcaat tgcctgctct ggcgagacgt cccaggcgtg agactgtgcc aaatgctcca
     1321 ttatgcttgt ggtgggtgat ctaactattt acgcggtgct gtctgatagg aggtttttta
     1381 gagatgccca tgcccatttc tggcttcggc tgccttttct aacaacgctt ccagggacag
     1441 gtcttctagc gatttgagca gcaggttggc gtggtcgagg ggcagtaggc gggtgagctg
     1501 gttgggcacg gctacacaga acaatccagc acgccgggcg gcggtgaccc caggggacga
     1561 atcttccagg acgatggcct ggtcgggcgt cagctcaagc gcggccaaca cagagtaata
     1621 tagttccggc tcgggcttta ggcgggtcac attttcccgt gtgcggatgc aatcaaagta
     1681 atcgatcagc ccacgctggg ttaggtgccc gccgacccac tcccaaggcg aactggatgc
     1741 cagaccgatc ttaagaccca agcggcgggc gtcgtccagg tatgagagca ctccaggcag
     1801 gaggggttgg gctgcgatca gctccgtctc ccgctgccag cgggcatcgt ttaatttttt
     1861 ccggtccagc ttgcgaccaa tttgaacctc cagttcttca acaaagtccc gctcattgtc
     1921 catggtgccg acatagtgca cccaatctga ggtctctagc ctgcctccct gagagtggaa
     1981 cagttcctgc caggactgga agatcggata ttcggtgtcc atgattaagc catcaaagtc
     2041 aaatattacg cttctaatta atgtgttctt catgcgtcta ctcgttggtc tgttatcctg
     2101 gtttttgact agatcaatta tggcaggctg cctcaaggcg agattatatc attttcaatt
     2161 ttgtctcgtt gactgaagcc ccattaccat gtacaatttc tggggaggtg gacatggatc
     2221 aggttctcaa agagatggcg ctccctggcg attgcctgct ccagatcgtc cagggtgaca
     2281 tcacccagga gagggtggac gccatcgtca acgcggccaa cgctcaactg cagcacggag
     2341 ggggcgtagc gggtgtgatc ttgcgccgtg gtggtgagga gatccaggca gagagcgatg
     2401 actgggtgcg ccagcatggc gcggtgatcc acgccgagcc ggcctacact accgccgggc
     2461 gactgtcctg ccgctacgtg atccacgccg tgggacccgt atggggctca ggcgatgagg
     2521 acgccaaact ggctgcggca atcgcgggct cactcagatt agcggaccag cttgagctgg
     2581 catcgattgc attcccagcg atctcaacag gtatcttcgg ctttcccaag aaacgcgccg
     2641 catgcgtaat tctgaacgaa atccgtatct actttgaaaa ccagcgtgat tcaggattga
     2701 agcaagtacg cctgacgctg ttcgaccaag agacaacgga tgcgttctta gaggaagctg
     2761 agggccaaag ctggggatga ttacctctgt ccataatccc aagatcaagt gggtacgctc
     2821 cctgcagagc gatgcgcgca cccggcggga agaggctgct tttgccgtgg agggggtcag
     2881 gctggcagac gaagccctgg cagccggatg gcaggcgcga ttggtgctgt tcagcgccga
     2941 tctggtggca cgcggcaagt cgattgtgga tggttttgcc gcccaggggg cgccggttga
     3001 agaggtggct gagcatgtca tgcgcgccgc cagtgacacg cagactcccc agggcatcct
     3061 ggtggtgctg gcgatgcgct cgctcccctt gccgcatgca ccggggctgg cttttatccc
     3121 agacgcaatc cgcgatccgg gcaacctggg cagcatgctg cgcaccgcag cagcagccgg
     3181 tgttgaggcg gttttgatcc cgcccggcaa tgtggacgtc tatgctccca aggtggtgcg
     3241 ggcagctatg ggtgcacact tccgcctgcc catcgtcagc ctgtcctggg acgaaatcac
     3301 tgcttacgtg gggatggctg gcttgcagct cgtgctggca gacgcagccg ggggtgaaat
     3361 gcacactcgc tcagacctgc gccatcctct ggctctcatc ctgggcggcg aggcagaggg
     3421 agccagccag gaggcgcgac gtctggcgca ggcgcgcctg catattccta tggccggcgg
     3481 agtggaatcg ttgaacgccg cggccgcagc ggcagtgctg ctcttcgaag tcgtccggca
     3541 gagaactcag gccgggaaat aatccgaaga ggaagta
//
LOCUS       DBNHOH010000024         1134 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_706231, whole genome shotgun sequence.
ACCESSION   DBNHOH010000024 DBNHOH010000000
VERSION     DBNHOH010000024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_706231"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="AC16V7_00395"
     CDS             <1..768
                     /locus_tag="AC16V7_00395"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015008065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR6255605.1"
                     /translation="ALAGLLPVPEIQFRKYAEPAQEQLSDLGTIRWRTAGKFAAPVVV
                     RIPVGYGKKVGDPWHSVSGEATFAHMVGWRIAFPSNAEDAVGLLRAALRGDDPSFFFE
                     HRALLDTPEGRRPYPGDDYCLPFGRAARLLQGNELAVITWGAMVSRCLDAAQAFSGRV
                     QVIDLRTIIPWDQETVLDSVRQTGKILVVHEDTQTGGFAGEIIAVIAEQAFHDLDAPI
                     ARLAMPDIPIPYNLSVLDALLPSVEKIRHKIEQMLAY"
     gene            780..>1134
                     /locus_tag="AC16V7_00400"
     CDS             780..>1134
                     /locus_tag="AC16V7_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6255606.1"
                     /translation="MNDLPFVLIERDPPIAIVRLNRPEVLNALSLEMFSDLCLALETL
                     DNEADIRVIIITGNERAFAAGADIKMLAQASPMDILRMDTLGYWQRIRRLKKPLIAAV
                     SGYVYGGGCELTLACD"
BASE COUNT          233 a          315 c          316 g          270 t
ORIGIN      
        1 gccttggcgg gtctgcttcc tgttcctgaa attcaatttc gcaagtacgc ggagcccgcc
       61 caggaacagc tatccgatct tggcacaatc cgctggcgaa cagcgggcaa gttcgctgcg
      121 cctgtggtgg tgcgaattcc agtgggttat gggaaaaagg tgggcgatcc atggcacagt
      181 gtgagcggcg aagcgacctt cgcccacatg gtaggctggc gtatcgcatt tccttccaac
      241 gccgaagacg ccgtcggttt gctgcgcgcc gctctgcgtg gcgatgaccc cagctttttc
      301 ttcgaacacc gcgccctatt ggacacccca gaaggtcgcc gcccttaccc gggggatgat
      361 tactgcctgc cttttggccg cgctgctcgt cttttacagg gcaatgagtt agccgtcatc
      421 acctggggcg caatggtttc tcgttgcctg gatgcggccc aggcattttc cgggcgcgtg
      481 caggtaatcg atctgcgcac cattatcccc tgggatcagg agactgtgct ggatagcgtt
      541 cgtcagactg gtaaaatcct ggtagtccat gaagataccc aaactggtgg ctttgccggg
      601 gagatcatcg ctgttatcgc tgagcaagct tttcacgact tagatgctcc cattgcccgc
      661 ttggcaatgc cggatattcc aattccctac aacctgagcg ttttggatgc tttgctacca
      721 tctgtagaaa aaattcgtca caaaatagag caaatgttgg catattagga agatgcgtta
      781 tgaatgattt acccttcgtc ttaattgaac gcgacccacc catagccatt gtgcgcctca
      841 accgaccgga ggtgctcaac gccctaagct tagaaatgtt ctccgacctg tgcctggcgt
      901 tggagacgct ggataatgag gcggatatcc gtgtgatcat catcactggc aatgagcgcg
      961 ctttcgcagc tggggcagat atcaagatgc tcgctcaagc atcaccgatg gacatcctgc
     1021 gcatggacac gctgggctac tggcagcgca tacgccgcct caaaaagcca cttatcgcag
     1081 cggtgagcgg gtatgtttac ggcggtggtt gtgagctcac cctggcatgc gacc
//
LOCUS       DBNHOH010000025         1425 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_577930, whole genome shotgun sequence.
ACCESSION   DBNHOH010000025 DBNHOH010000000
VERSION     DBNHOH010000025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_577930"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="AC16V7_00405"
     CDS             <1..360
                     /locus_tag="AC16V7_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR6255607.1"
                     /translation="PLDGDSIPGAIQQLLEHPLVNTKVTPSSEGAVQQSALQGGANAT
                     HLSDPAFDTADFADGAPGNLRADYVLPRKNLRIADAAVFWPLNTDSLFPLVGTFPFPS
                     SDHRLVWVDAWVPGSQK"
     gene            611..>1425
                     /gene="ppk2"
                     /locus_tag="AC16V7_00410"
     CDS             611..>1425
                     /gene="ppk2"
                     /locus_tag="AC16V7_00410"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006104986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HIR6255608.1"
                     /translation="MAKKNKNDKGKEILREKPPKQSEPIAAPLVVEAEPAKELEKKLR
                     NKEYLKELGQLQIELVKLQEWIKLKGLKLVVIFEGRDAAGKGGVIKRITECLNPRYAR
                     VVALPAPTEREKTQWYFQRYVAHLPAAGEMVFFDRSWYNRAGVERVMGFCTNEEYQEF
                     LRACPEFERMLVRSGIILVKYWFSVSDEEQERRFQARIEDPTKRWKLSPMDLESRTKW
                     VEYSIAKDEMFKYTDIKQAPWYVVNSDNKKRARLNCINHLLSVIPYQDLTPEP"
BASE COUNT          353 a          411 c          374 g          287 t
ORIGIN      
        1 ccattagatg gcgacagcat ccctggagcc atccagcaac tgctggagca cccgctggtc
       61 aataccaagg tgacgcccag cagcgagggt gcagtccagc agtcggcgct gcagggcggt
      121 gcgaacgcta cgcacctgag cgatccagcc ttcgatacgg ccgactttgc cgatggggca
      181 cctggtaacc tgcgcgccga ctacgtgctg ccgcgcaaga acctgcgtat cgccgacgcc
      241 gctgtattct ggcctctaaa cactgattcg ctcttcccac tggtcggcac cttccccttc
      301 cccagctccg accaccggtt ggtgtgggtg gatgcttggg tgcccggttc ccaaaaataa
      361 tcctacctca attgcatcct tgtgcctggc atgtttattg tgccaggcgc aatttgatta
      421 agtgttacgc gtccacacgg agagtgaccc attaaaacca acaaccaatc ccggtttttt
      481 ctgcctgatt ttcgacaatg tccaaatctt gtccaagcat gagccgctcc ggttgacaaa
      541 gggggaatct cccctataat aaatggcagc taacaatcgt caatccttat aattttctcg
      601 ggaggcaacg atggcaaaaa agaataaaaa cgataaaggg aaggaaatcc tgagggaaaa
      661 gccacccaag caatctgaac ccatcgccgc gcctctcgtt gttgaagccg agcccgccaa
      721 ggaacttgag aaaaaactgc gcaataaaga atatctcaaa gaactcggtc aattacagat
      781 cgagctggtc aaattgcaag aatggatcaa gctcaaaggc ttaaagctgg tggtgatctt
      841 tgaaggccgc gacgcggctg gcaagggggg cgtgatcaag cgcatcaccg agtgcctgaa
      901 ccctcgctat gcccgcgtgg tggcattgcc cgcccctaca gagcgcgaga aaacgcaatg
      961 gtacttccag cgttacgtgg cgcatctccc ggcggctggc gagatggtct tctttgaccg
     1021 cagctggtac aaccgggctg gcgttgagcg cgtcatgggc ttttgcacca atgaagaata
     1081 ccaggaattt ctgcgcgcct gccccgagtt tgagcgcatg ctggtgcgct cagggatcat
     1141 cctggtcaaa tactggttct cggtcagcga tgaagagcag gagcgccgct tccaggcgcg
     1201 tatcgaagac cccaccaagc gctggaagct cagcccaatg gacctcgaat cgcgcacaaa
     1261 gtgggtagaa tactccatcg ccaaggatga gatgttcaag tacaccgata tcaagcaggc
     1321 accctggtac gtggtcaact ctgacaataa aaaacgtgcc cgcctgaact gcatcaacca
     1381 cctgctgagc gtcataccct atcaagacct gacacccgag ccgat
//
LOCUS       DBNHOH010000026         2655 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1667617, whole genome shotgun sequence.
ACCESSION   DBNHOH010000026 DBNHOH010000000
VERSION     DBNHOH010000026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1667617"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..445
                     /locus_tag="AC16V7_00415"
     CDS             257..445
                     /locus_tag="AC16V7_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large ribosomal subunit protein bL28"
                     /protein_id="HIR6255609.1"
                     /translation="MAKCAHCGKTTAFGRNRPWSKKATPRTFRPNLQTISVFENGRKN
                     RKVLCAKCIRTLVKTENE"
     gene            complement(499..1608)
                     /locus_tag="AC16V7_00420"
     CDS             complement(499..1608)
                     /locus_tag="AC16V7_00420"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR6255610.1"
                     /translation="MPDPILLNNRYQLEERLGYGGMAVVYRAQDLMLERSVAIKILRE
                     NFSGDVDFRERFRQEAKAAANLTHPNIVTIHDFGYDSDRLFIVMEYVPGTHLKSILQR
                     RGRLSVNEAVTLMVQACAGVGYAHRAGLVHCDIKPQNFLITPDKRLKVTDFGIARALA
                     SIQPEEKSEIVWGSPQYFSPEQASGGPPSPASDVYSLGIILYELLTGSIPFLAESPTE
                     LARMHRDDPPPLPRDLNPSISPELEQIMLKVLSKEPSARYRTADQFGRVLVSFSGQNN
                     LATMIVDSEGGSASYAVLPAAEQTASATSPHTPPNQTSRQAYSRPAEPPVTQYTSAQD
                     NPLEVDWITIFLGLIALIFVGGLIPFYLWVYYLFF"
     gene            1721..>2655
                     /locus_tag="AC16V7_00425"
     CDS             1721..>2655
                     /locus_tag="AC16V7_00425"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR6255611.1"
                     /translation="MPNGMIAYNPNAGRYPSRILTERARDVLREYGWELILEQTTSGD
                     HTTQLAKKAADEGMDAIFIAGGDGSVNRAMRALIGTDTALGVLPCGTANVWAQELGLP
                     GLTWTRWMALEESARRLATAPICAVDIGLCNNRPFLLWAGVGLDAFVVHNLEPRSRWE
                     KHFAVAHYAASIVWHASYWHGVNIRIETDAGRIFGHFLLALVSNVHLYAGGYANLSPH
                     ARLDDGSMDLWLFKGETLGDTVQLVWDLLAGRHIQSERVHQVQFQSLRLESESPTYVQ
                     VDGEPIDVVSPVTITVQPKALRVLVPESTPQPLFNT"
BASE COUNT          636 a          603 c          754 g          662 t
ORIGIN      
        1 gttcattagg ctcttgtgag ccagacccac cacgccgtac gattcgcggg cggcatggta
       61 agctattgat gcaatggcgc gaggtgagac attgatgctt cccatgggat tagtttcgcc
      121 ggtcataatg attccttttc aaattggctt gtataaagca gcctgatgtg gtaagattgc
      181 acggctattg accgagaggt tggagtccat tcgttgagca ttggattcac ccaaattata
      241 ataaggaagt gttattatgg cgaagtgtgc acattgtggt aaaacaactg cgtttggccg
      301 caaccgtccc tggtcgaaga aagcgacccc caggacgttt cgaccaaact tgcagaccat
      361 aagcgtcttc gagaacgggc gcaagaaccg caaggttttg tgtgccaaat gcatccgcac
      421 cctggtaaag accgaaaacg agtgatcgaa tagctcctga ctttaaaaat cgcggcgcaa
      481 gccgtgattt ttgttttctt agaagaatag ataatatacc cataaataaa aggggatcaa
      541 cccccccacg aaaattaagg ctatcaggcc caaaaaaatc gtaatccaat cgacctcaag
      601 gggattatct tgagctgaag tgtattgtgt tactgggggt tcagctggac gggaatatgc
      661 ctgccgagag gtctggttag gcggagtatg cggcgatgta gcggatgcgg tttgctctgc
      721 ggcgggcagg acagcgtaag aggcagagcc tccctccgag tcaacaatca tcgtagctaa
      781 gttgttttgc ccgctgaaac tgaccaagac cctgccaaat tggtcggcgg ttcgataacg
      841 ggctgatggt tctttggaga gaaccttgag cataatttgt tctagctctg gggagatcga
      901 tggattcaag tcgcgcggca ggggtggcgg atcatcccga tgcattcgtg ccagctcggt
      961 tggggattca gccaggaagg gtatgctgcc ggtgagaagc tcataaagaa tgatacccag
     1021 agagtatacg tctgaggcag gcgatggagg tcccccggag gcttgttcgg gggaaaaata
     1081 ctgcggagaa ccccagacga tttcgctctt ttcctccggc tggatggaag ccaaggcgcg
     1141 ggcgatgcca aaatcggtta ctttcaagcg tttatcgggg gttatgagga aattttgggg
     1201 ctttatgtca caatgtacca gcccagcgcg gtgggcataa ccgacccccg cgcaggcttg
     1261 caccataagg gttactgcct cgttgaccga gagccgaccg cgccgctgca agatagattt
     1321 cagatgggtt cccggcacgt actccatgac aatgaacagc cggtccgaat cgtatccaaa
     1381 atcatgtata gtgacgatat tgggatgggt cagatttgcg gcagccttgg cctcctggcg
     1441 gaaacgctcg cggaaatcga catcgccgct gaagttttcc ctcaagatct ttatggcgac
     1501 actacgttcc aacattaagt cttgggcgcg gtatacgacc gccatcccgc catagcccag
     1561 gcgctcttct aattggtatc ggttattaag taatatgggg tctggcatta atacatccac
     1621 ttgccgctat caggtagaat tgtaccaccg caatttctga agagcagata ttatatccag
     1681 tttttcgttt aaaatcatgt aattgtacag gtgtttttgt atgcctaacg gaatgattgc
     1741 atataacccg aatgctgggc gttacccatc acgaatactg accgaacgtg cgcgtgacgt
     1801 gctccgtgag tatgggtggg agctaatcct ggaacaaaca acaagtggcg atcataccac
     1861 tcagcttgct aaaaaagccg ctgacgaggg aatggatgcg atttttatcg ctggaggaga
     1921 tggctcggtc aatcgagcaa tgagggcatt gattggtaca gatacagctc tgggtgtgct
     1981 gccttgtgga acagcaaacg tttgggcgca ggaacttgga cttccggggc ttacttggac
     2041 acgctggatg gctctcgagg agagtgccag gcgcttagct actgctccta tttgtgctgt
     2101 ggatataggt ttgtgcaaca accgaccgtt tttattgtgg gccggtgttg ggctggatgc
     2161 ttttgtcgtc cacaatcttg agcctcgtag tcgctgggaa aagcatttcg cggtggcgca
     2221 ctatgccgct tcaattgtat ggcatgccag ctattggcat ggggtgaata ttcggattga
     2281 aaccgatgca ggtcgaattt tcggtcattt cctgctggca ttggtgagta atgtgcatct
     2341 ttacgcggga ggttacgcga atctgtcacc gcacgccagg ttggacgatg gatcgatgga
     2401 tttgtggcta ttcaaaggag agacgctggg tgataccgtc cagttagtat gggatttgct
     2461 ggcgggtagg catattcaat ctgagcgggt gcatcaggta caattccaaa gtctcaggtt
     2521 agaatccgag tcgccaactt atgtccaggt ggatggcgag cctatcgatg tggtgagtcc
     2581 ggttacaatt actgtgcagc ccaaagcgct acgcgtcctt gtgcccgaga gcacacccca
     2641 gccgttgttt aatac
//
LOCUS       DBNHOH010000027         3516 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_257359, whole genome shotgun sequence.
ACCESSION   DBNHOH010000027 DBNHOH010000000
VERSION     DBNHOH010000027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_257359"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1946
                     /locus_tag="AC16V7_00430"
     CDS             <1..1946
                     /locus_tag="AC16V7_00430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR6255612.1"
                     /translation="EFGASPDAPLLHPDLGKYEVANFIFPEPGTNISNHFRIRKGDVE
                     AAWLECEAIVERSYRIPHVQHVPIEPHVATAKVDEQGRVTLWGSSQSPFAQRNLIAKS
                     LGKSQSDVQVVAPYVGGGFGSKAGVSMEALAVAIAMKVPGRPVKLRLTREEEFYTAFV
                     RQSLVSHFKMGCDKEGRLLAMEVKFYWDGGAYTEYGVNITRASGYSSSGPYEVPNIKT
                     DSYCVYTNHPVSGPMRGFGMPEMHSGLEQCIDELAHAIKMDPVQFRKINCVKKGSTLI
                     TGMVMHPTGLSECLTKAAEAIGWGKKDKPSAPNKRRGKGIALMWKAPAMPPNAGSSAK
                     VALNEDGTLTVSVGGQEIGQGSFTIAAQMAAGALGVPWDWVRVATPLNTDYSPYEWQT
                     VASRLTWSMGNAIVAAARDARKQILDMVAKAWSENPDDLDIIDGKVISYKSEESISLK
                     DIVIYGISLPDDQGWIGGPVMGRGNFMPTYVTGLDPETGQGPRAVVHYTTGAQAVEIE
                     VDMDTGKVDVIRGVAAFDVGKAINPDMVKAQMEGGFVQGLSTALFEDLKLKEGFVQNS
                     SFVDYRIATSSDAPQSIEAIIVEVPQDDGPWGARGIGEHSMVPTIPAIANAIYDAIGI
                     RPGAPPYTSEKIYLAMQDAGLVD"
     gene            2310..>3516
                     /locus_tag="AC16V7_00435"
     CDS             2310..>3516
                     /locus_tag="AC16V7_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6255613.1"
                     /translation="MRPLKDLRVLDVSRALAGPYCTMIFGDLGADVIKVELPGRRDES
                     RNWGPPFVGEPYEVYPGESSYFMAINRNKRSITVNLKTAEGQEIVKRLAAVSDVFVEN
                     YRTGTLEKMGLGYEDLHALNPRLIYCSISGYGRNGPYAERPGYDFIIQAEGGIMGVTG
                     PEDGSPYRVGISIIDITTGIFASTAILAALHARELSGEGQLIDISLLDTSVALLSNVA
                     ANYLVAGVEPKRMGNAHFNIAPYEVFRARDKWFTLGAANVRQWEMLCEVVGKPDLKND
                     PRFTTNKDRVANRGALSKILNDAFAQQDAEFWLSKLQEAEIPSGPINTIADVFDHPQA
                     MARDFSVQIDHPTAGSINLPGFPYKLSQTPPEMYRHPPRLGEHTSEVLAKVLGYTLEN
                     IEHLKEQKVI"
BASE COUNT          881 a          885 c          962 g          788 t
ORIGIN      
        1 cggagtttgg tgccagcccg gacgcaccac tgctacaccc tgatttgggc aaatacgaag
       61 ttgccaattt cattttccca gaaccaggca ctaatatctc caaccatttc cgcatccgca
      121 agggagacgt ggaagcagcc tggcttgagt gcgaagcaat cgtcgagcga agttaccgca
      181 tcccccatgt gcagcacgtc ccgatcgagc cgcatgttgc cacagcaaag gtggatgaac
      241 aaggtcgggt gacgttgtgg ggaagctctc agtctccctt cgcgcagcgc aacctgatcg
      301 ctaagtcttt ggggaaatcc caatctgatg tgcaggtggt tgccccatac gtgggcggcg
      361 gtttcggcag caaggctggg gtcagcatgg aagccttagc tgttgccatc gccatgaagg
      421 tcccagggcg ccctgtgaaa ctgcgcttga cacgtgaaga ggaattctac actgcctttg
      481 tgcggcagag tctggtttct catttcaaga tgggttgtga caaagaaggt cgcctgctgg
      541 cgatggaggt aaagttctac tgggatggag gcgcttacac cgagtacgga gtgaacatca
      601 cccgcgcttc cggttacagc agcagcggac cctacgaggt gccgaacatc aaaacagaca
      661 gctactgtgt ctataccaac cacccggtaa gcgggccgat gcgcggcttt ggcatgcctg
      721 agatgcattc cggtttggag caatgcatcg atgagctggc acatgctatt aagatggacc
      781 ctgtacaatt tcgaaaaatc aattgcgtca agaagggcag cacgctcatc accggcatgg
      841 taatgcaccc caccggcttg agtgaatgcc tcacaaaagc agctgaagcc ataggatggg
      901 gcaaaaagga caaaccgagc gccccgaata agcgacgcgg caagggcatt gccttaatgt
      961 ggaaagcccc cgccatgccg cccaatgccg gctccagtgc aaaagtggca ttaaacgagg
     1021 atggcaccct gactgtcagt gtaggtgggc aggagattgg acagggttcg ttcaccattg
     1081 ccgcccagat ggcagcaggt gccctggggg tgccatggga ctgggtgcga gtggctactc
     1141 cgctcaatac cgattacagc ccttatgagt ggcaaaccgt cgccagccgc ctgacgtgga
     1201 gcatgggcaa tgctatcgtt gccgcggcgc gcgacgcccg caaacagatc ctcgatatgg
     1261 tcgccaaagc ctggtcggag aaccccgacg acctcgacat cattgatgga aaggtgatct
     1321 cctataaatc cgaggagtcc atctctctaa aagacatcgt gatctatggc atctcactgc
     1381 cagatgatca gggctggatc ggcggtccgg tcatgggacg tggcaatttt atgcccacct
     1441 atgtgactgg acttgaccct gagaccggac agggaccacg agctgtcgtc cactatacaa
     1501 ccggagccca ggcagtagag atcgaagtgg atatggacac cggaaaagtg gatgtgatcc
     1561 gcggggtagc agctttcgat gttggcaagg ctatcaaccc ggatatggtc aaggcacaga
     1621 tggaaggggg ctttgtccag gggctgagta cagccctgtt cgaagactta aagctcaaag
     1681 aaggcttcgt gcagaactcc agttttgtag attatcgcat agctaccagc tcagatgcgc
     1741 ctcaatccat cgaggctatt atcgttgagg tcccgcaaga tgatggcccg tggggcgcgc
     1801 gcggaattgg cgaacattcc atggttccaa cgattccggc aattgcaaac gccatttacg
     1861 atgccattgg catccgcccc ggcgccccgc cctatacctc ggaaaagatt tacctggcaa
     1921 tgcaggatgc cgggcttgtg gattaactat tgacgtgatt tccaagtgag gtgcggccgc
     1981 cagatcaaac ggtgctagcg tggaagtaag acagattgat ttcccatcca attctgcgta
     2041 taatgaaccc ggaaattgga tcttagattc ccattgacaa tgtcgctgat ccacatttcg
     2101 cctatgacac tgatggattt cccttctcac agttcttcta caggccatat ttttaaaatt
     2161 acaccaaacg ggtgagctca ccgcacagta gaccaacccg tcttaatgtc atacgtgtaa
     2221 agacgtagcg gtaaagttag cgaaaaacct cttatcatga acgacctaat acctgaaaaa
     2281 atccgggtat tcatcatggg aaggattgca tgcgtccatt gaaagatttg agggtactgg
     2341 atgtttcacg tgctttagct ggtccatact gtacgatgat ctttggcgac ctcggagctg
     2401 acgtgatcaa ggtggaactt ccaggtcgac gcgacgaaag tcgcaattgg ggtccgccct
     2461 ttgtcggtga gccatacgag gtttatcccg gtgaatcgtc ttatttcatg gcgatcaatc
     2521 gcaataaacg aagcatcacg gttaatttga agactgctga aggccaagag attgtcaagc
     2581 gcctggcagc tgtctccgat gtgtttgtgg agaactatcg aactggcaca ttagaaaaga
     2641 tgggattagg ttatgaagac cttcatgccc tgaatccacg cttgatatat tgctcaatta
     2701 gtggatacgg ccggaatggc ccttatgctg agagacctgg ttacgacttc atcatccaag
     2761 cagaaggcgg gattatggga gttaccggcc cggaagatgg ctctccctac cgtgttggca
     2821 tatccatcat tgatattacg acggggatat ttgcctccac ggcgattttg gccgctttgc
     2881 acgcgcggga attatccgga gaaggccagt taattgatat ctcgctcctc gatacttccg
     2941 ttgcgctcct ctcgaatgtc gctgcaaatt acctggtagc aggggtagaa ccgaagcgca
     3001 tgggcaatgc acacttcaac attgctcctt atgaagtttt ccgcgctcgg gataagtggt
     3061 ttaccctggg tgcggcgaat gttcgacaat gggagatgct atgcgaagta gttggcaagc
     3121 cagacctaaa aaatgatccg cggtttacaa ccaacaagga ccgggtagcc aaccgtggtg
     3181 cattgtcgaa aatattgaac gatgctttcg ctcaacaaga tgctgagttt tggttgagca
     3241 agctgcaaga agctgaaatc ccaagcggac cgatcaacac catcgcagat gttttcgatc
     3301 atccgcaagc aatggcgagg gatttttcag tgcagataga ccatcccacg gcggggtcga
     3361 tcaacctgcc cggttttccg tacaagctat cgcaaactcc cccagagatg tatcgacacc
     3421 ctccaagatt gggagagcac acaagcgaag tgctggcaaa ggtgttagga tatactcttg
     3481 agaatattga gcacctaaaa gagcagaagg tgattt
//
LOCUS       DBNHOH010000028         2702 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1603416, whole genome shotgun sequence.
ACCESSION   DBNHOH010000028 DBNHOH010000000
VERSION     DBNHOH010000028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1603416"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="AC16V7_00440"
     CDS             <1..400
                     /locus_tag="AC16V7_00440"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR6255614.1"
                     /translation="AVANAFHARTETITPVSARTLADSISVDLPRDGVRAVRAARDTG
                     GSYIVVSDEDIISSIAALGQVGIFAEPAGATSHAGLVKAIGMGLIDPDDPVIVINTGS
                     GLKDVKAAMQAAGQAPIIEPTLEAVRGVIK"
     gene            405..1349
                     /locus_tag="AC16V7_00445"
     CDS             405..1349
                     /locus_tag="AC16V7_00445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR6255615.1"
                     /translation="MTPSISVIAPIFNEEKNIPELYRRVKETLDLAGETWELLMVDDG
                     STDQSSELIRQLNHQDQRVLPVIFARNFGHQIAVSAGMDYSRGQAVVIIDSDLQDPPE
                     VILDLIEKWREGYEVVYAVRTEREGESWFKLFTASLFYRLIYRITDVEIPMDAGDFRL
                     LDRKVVNVLKGMRERFRFLRGMSAWVGFRQVGVPYRRAARMAGETKYPFRKMFRLALN
                     AITGFSYFPLQLATYLGFVSAGISILTIPVVVSMRLTGSQAFFGQATTLIAVLFLGGV
                     QLISLGILGEYIGRLYDEAKGRPLYIVREEPPNIDDHD"
     gene            1468..>2702
                     /locus_tag="AC16V7_00450"
     CDS             1468..>2702
                     /locus_tag="AC16V7_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR6255616.1"
                     /translation="MKIDLTMEKDRRSRAIQRVRERNIIIPTFAQMKNPDKIPARVKE
                     ELRNVGLWDLDPRNLFRISWHNEPSPQGGGFGRVNYLEFPKSLTGTEARVIALVGKWF
                     PTGAHKVGAAFGCLVPRLVSGQFEPTTQKSVWPSTGNFCRGGAYDSALLACQSIAILP
                     EGMSRERFEWLSKIAGEVIATPGSESNVKEIFDKCWELRASGQDLMIFNQFEEFGNYL
                     WHYEITGHAMQEVLEEVMGPRDAYRGMVSATGSAGTIASGDYMKQLFPASKIGASEAL
                     QCPTLLENGFGSHRIEGIGDKHVPWVHNVKNTDVVVAIDDNAVVNLARLFNEPAGRAY
                     LVKQGVPEALVGKLDLLGFSGISNMLSVIKMAKYYEMGENDIVLTVLTDSMELYGSRL
                     REMHDEYGAYSDLDAAAHFA"
BASE COUNT          612 a          617 c          778 g          695 t
ORIGIN      
        1 cgccgttgcg aatgctttcc atgccaggac ggagacgatc accccggttt ctgcccgcac
       61 acttgccgat agcatctctg tagacctgcc gcgagatggt gtgcgcgccg tgcgcgccgc
      121 tcgcgatacg ggcggatcgt atattgtcgt cagcgacgag gatatcatct catctatcgc
      181 ggccttaggt caggtaggta tttttgctga gcctgctgga gctacgtcac atgctggatt
      241 ggtcaaggcg attggcatgg ggctgattga tccggatgat ccagtaatcg tgattaacac
      301 cggtagcggt ttgaaagatg tcaaagctgc catgcaagcc gctggtcaag ccccgattat
      361 agagcctacc ctcgaggcgg tcaggggggt gataaaataa atatatgaca ccctccatct
      421 cggttatcgc tccaattttc aacgaagaga aaaacatccc tgagctttac cgccgcgtga
      481 aagaaacttt ggatttggct ggagagacct gggagctctt aatggtcgat gatggctcta
      541 ccgatcaatc ttctgagttg atccgccagc ttaatcatca agaccagcgc gtgcttccag
      601 tgatcttcgc tcgtaatttt gggcaccaga ttgctgtctc tgctgggatg gattactcgc
      661 gtggtcaggc tgtggtgatc atcgattcag accttcaaga tccgccggaa gtcatcctgg
      721 atttgattga gaaatggcgc gagggttatg aggtggtcta cgcggtgcgc accgagcggg
      781 aaggggaaag ctggttcaaa ctcttcactg cctcactatt ttatcgcttg atctatcgca
      841 tcaccgacgt agaaattccg atggatgcag gtgatttccg tttattggat cgcaaagtgg
      901 tgaatgtgtt gaagggtatg cgtgagcgtt ttcgctttct gcggggaatg tcggcctggg
      961 tgggttttcg ccaggttggc gtcccttacc gccgggcggc gcgtatggct ggcgagacta
     1021 aatacccttt ccgcaaaatg ttccgcttgg cgcttaatgc cattactggt ttttcctatt
     1081 ttccattgca gctggctaca tatctggggt ttgtatctgc agggatcagc atcttgacta
     1141 tcccggtagt tgttagcatg cgtcttaccg gatcacaggc attctttggg caggcaacaa
     1201 ccctgattgc tgtcttgttc ctgggtggag tccagctgat ctccttaggg atcttggggg
     1261 agtacatcgg gcggttatac gatgaggcca aggggaggcc attgtatatt gtgcgtgagg
     1321 aacctccgaa tattgacgat cacgattaag cattaagata ggtaaaaagc aatagcattg
     1381 tttcatgtga tttctgatac taggattatt gttaacttat ctttgttcaa aaatttcact
     1441 gagaagattg atgaggagaa aacctaaatg aaaatcgacc ttacaatgga aaaagatcgc
     1501 agatcgcgcg caatacaacg cgtgcgtgag cgcaatatta ttattccgac ttttgcccag
     1561 atgaaaaatc cagacaaaat ccctgccagg gtgaaagaag aactgcgaaa tgttggtcta
     1621 tgggatttag acccacgcaa cctgttccgt atttcatggc ataacgagcc atccccgcaa
     1681 ggtggtggat ttggccgggt aaattacctg gagtttccca agagcttgac cggaactgag
     1741 gcgcgtgtca ttgccttggt agggaaatgg tttcccacgg gtgctcataa agttggcgca
     1801 gcatttgggt gcctggtgcc acgcttggtc agtggccagt ttgagcccac aacacagaag
     1861 tctgtatggc cgtctactgg taacttctgc cgcggtggcg cctatgactc agccctcttg
     1921 gcttgtcaat cgattgcgat cttgccggag gggatgagcc gcgagcgttt cgaatggctt
     1981 tcgaaaattg ctggtgaggt gattgctacg cctggcagcg agtcgaatgt caaagagata
     2041 ttcgataagt gctgggaatt acgcgcctct gggcaggact tgatgatctt caaccagttc
     2101 gaggagtttg ggaattattt gtggcactac gagatcactg ggcatgccat gcaagaggtg
     2161 ttggaggaag tcatgggacc ccgtgatgcc tatcgcggca tggtctctgc caccggttcg
     2221 gctgggacga ttgccagcgg ggattacatg aaacaactct tcccagccag caagatcggc
     2281 gctagcgagg cattgcagtg cccgactctg ctggagaacg ggttcggttc ccatcgaatc
     2341 gaaggcattg gagataagca cgtaccttgg gtgcataatg taaagaacac cgatgtggtc
     2401 gtggcgattg acgataacgc ggtagtcaac ctggcgcgcc tgttcaacga gccggctggg
     2461 cgggcttact tggtgaagca gggtgtgcca gaagccctgg taggcaaact ggacttgttg
     2521 ggcttctcgg gcatctcgaa tatgctctcg gtgatcaaga tggccaagta ctacgagatg
     2581 ggtgagaatg atatcgtatt gaccgtgctg accgattcaa tggagctata tggcagccgc
     2641 ctgcgagaga tgcatgatga gtacggtgct tatagcgatc tagatgcagc agctcatttt
     2701 gc
//
LOCUS       DBNHOH010000029         1741 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_129608, whole genome shotgun sequence.
ACCESSION   DBNHOH010000029 DBNHOH010000000
VERSION     DBNHOH010000029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_129608"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="AC16V7_00455"
     CDS             <1..967
                     /locus_tag="AC16V7_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR6255617.1"
                     /translation="VKDLASRDVISRAMYLEMKEGRGIGGKRYLYLDVRPETVNKYAA
                     KDGRKRPDGSPYSVTGDEILRKLPDIIDFCRTYLGVDPVQQPMPVQPTAHYTMGGIPT
                     NKYGEAVIDEKNAVLPGLFAAGEAACVSVHGANRLGTNSLLDLLVFGKHSGLRAAEYA
                     KGASFQSLPQDATEFARAQFDAILNNGGSEKAADIALEMKSVMNDHVSVFRTEEGMQT
                     ALEKVRELKERFKHVQVTDRGKIFNTELLTTWEIGNLIDLAEVTTVCALARKESRGAH
                     ARDDYPKRDDGNWMKHTLAWTRDDGIRLSYKPVVITKFQPKERVY"
     gene            982..1683
                     /locus_tag="AC16V7_00460"
     CDS             982..1683
                     /locus_tag="AC16V7_00460"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (quinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HIR6255618.1"
                     /translation="MQITLKVFRFNPEIDKKPHYEKYTLEAQPTDRVLDLLEHVKGYI
                     DGTLSYRRSCAHGICGSDAMRINGRNRLACKALVKDVGSKITVEPILGLRVIKDMIVD
                     MEPFFESYRSIMPYLVNDDPVPNQERLQSPEQRERFDDTTKCILCAACTTSCPSFWAS
                     DKYIGPASIVNAHRFIFDSRDQAGAERLSLLTDQWGVYRCHTIFNCTEACPREIQVTQ
                     AIGEVKQAIATGKID"
BASE COUNT          422 a          461 c          487 g          371 t
ORIGIN      
        1 ggtaaaagac ctcgcttcgc gtgatgtaat cagccgcgcc atgtatctcg agatgaaaga
       61 ggggcgcggc atcggcggta agcgctatct ctacttggac gttcgcccgg agacggtgaa
      121 taaatacgca gctaaagatg gccgcaagcg cccagatggt tcaccttact cggtaactgg
      181 tgacgagatt ctgcgcaagc tgccggacat catcgatttt tgccgcactt acctgggcgt
      241 ggacccggtg cagcaaccca tgcctgtgca gcctactgcc cactacacca tgggcggcat
      301 ccctaccaat aagtacggcg aggcagtcat tgacgagaaa aatgctgttc tgcctggcct
      361 gttcgccgct ggcgaggcag cctgcgtctc agtgcacggc gcaaatcgct tgggaaccaa
      421 ctcgctgctg gatctgctgg tctttggtaa gcattctggc ttgagggcag ccgaatatgc
      481 caagggagcc agcttccaga gcttacccca ggacgctacc gagtttgcgc gtgcccagtt
      541 tgatgccata ttgaacaacg ggggttccga aaaagccgcc gatattgctc tagagatgaa
      601 atcggtgatg aacgatcatg tgagtgtttt tcgcaccgaa gagggcatgc agaccgccct
      661 ggaaaaggtc cgcgagctga aagagcgctt taagcacgtc caggtaaccg atcgtggtaa
      721 gatatttaat accgagttgt tgacaacttg ggagatagga aacttgattg acctggcaga
      781 ggtaaccacg gtgtgtgcgc tggcgcgcaa ggagagccgc ggcgcgcatg cccgagatga
      841 ctatcccaag cgcgatgacg ggaactggat gaaacacacc ctcgcctgga ctcgggatga
      901 tgggatccgg ttaagttaca aaccggtggt gatcacaaaa ttccagccaa aagaacgggt
      961 ttactagaaa ggcgggcgag catgcagatt acactgaaag tctttcgctt caatccagaa
     1021 attgataaga agccccatta tgaaaaatat actttagagg ctcagcccac cgaccgggtc
     1081 ctagatttgc tggagcatgt caagggttac attgacggca cgctctctta tcggcgctcc
     1141 tgcgcccacg gcatctgtgg ctccgacgcc atgcgcatca atggacgtaa ccgcctggcg
     1201 tgtaaagcac tggtcaaaga tgtgggcagt aagatcaccg tcgagcccat cttgggattg
     1261 cgcgtgatca aggacatgat tgtagatatg gagccgttct tcgagagcta tcgctccatc
     1321 atgccctatc tggtcaacga tgacccagta cccaatcagg aacgcttgca gagccctgag
     1381 cagcgcgagc gcttcgacga tacaaccaag tgcattctgt gcgcagcctg cactacctcc
     1441 tgcccttcat tctgggcaag tgataaatat ataggacctg cgtcgatcgt caacgcccac
     1501 cgcttcatct tcgacagccg cgaccaggca ggtgccgagc gcctcagttt gctgactgac
     1561 caatggggtg tgtaccgctg ccatactatt ttcaactgta cagaagcctg tccgcgcgag
     1621 atacaggtga cgcaagccat cggcgaggta aaacaagcca tcgccacagg gaagatcgat
     1681 tagtgagaaa tttctcccgc agggtcagag cctgcgggaa gtttttttgt tggttatgct
     1741 a
//
LOCUS       DBNHOH010000030         3121 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1603462, whole genome shotgun sequence.
ACCESSION   DBNHOH010000030 DBNHOH010000000
VERSION     DBNHOH010000030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1603462"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="AC16V7_00465"
     CDS             <1..330
                     /locus_tag="AC16V7_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255619.1"
                     /translation="VSLMSDYQNQRLTPYLYRGRGCYDSQVQAAEYFFMESTSIESID
                     ALQLIGSEEVGENRWEIKFLSRADKTRYKFIVAAEKSAFEIFESCSKPDKSSSRLQYH
                     LVEWSQA"
     gene            327..947
                     /locus_tag="AC16V7_00470"
     CDS             327..947
                     /locus_tag="AC16V7_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HIR6255620.1"
                     /translation="MTNLTIYQIYDLLNRPRPLWLVKINLSETSLWMADLSECNLSWA
                     DLTRCDLSWAKLIKTDLSRANLSYANFYEAHLTHANLTEANLNRANLTRANLEKTNLY
                     HAVATEANFSRANLSRSNLKQANLSGAIFSRADLSKSDLINCNMNGAILTHTNLFEAN
                     LQGVDLKGANLENADLTASNLADANLHGANLTGANLTDAILPEGMK"
     gene            994..1560
                     /locus_tag="AC16V7_00475"
     CDS             994..1560
                     /locus_tag="AC16V7_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255621.1"
                     /translation="MSADQNDKRVGFLPFHAINEYMTEVYRLQVIRDVLTGLPNLPED
                     HRNKINQLTRKLVKVPGFRNSVKAPLPIRIKSTVPAFEKKPDLAAALLAAWADAKAEL
                     RERVYALLKSRNWELLPLETDRTKLPGFMTIWPGGENFEILNQAFGEMYPDFSTTNDD
                     VSLMVVWISTRLPYQVTDEGEVEVQTIR"
     gene            complement(1566..2675)
                     /locus_tag="AC16V7_00480"
     CDS             complement(1566..2675)
                     /locus_tag="AC16V7_00480"
                     /inference="COORDINATES: protein motif:HMM:NF027787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HIR6255622.1"
                     /translation="MDPYNSNPKRYSDRILIYILVAILLAILCMVCLGSGILIRRYAF
                     PDVKSTRTALAELFGTITPLVTPSLQLDTPQASPTKSPQPLPTQTQVPPKPTYSSPVW
                     LEPPPGKIVYACFVNGYDQICKMKADGSGIIQLTDEESTNFYPSFDPDGERIVFSSRR
                     DGNFEIYIMDVDGSNVHKLTDNIGNLYAPEISPNGKRIVFTAESGGKQAIWIMKIDGG
                     NARPISSDDIYGIDPTWSPSGDRIAFASIIGGATQLFIVNADGTKLKQVTKGISGIGG
                     RSTWSPDGEWLAFYAGPKGDRDIYMINVDDKELRKLTDGGDNLGPSFSPDGEWIAFTS
                     YRDGNNEVYVMRSDGSNATRLTGELRSDWQPRWGR"
     gene            2765..3070
                     /locus_tag="AC16V7_00485"
                     /pseudo
     CDS             2765..3070
                     /locus_tag="AC16V7_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906688.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
BASE COUNT          872 a          739 c          696 g          814 t
ORIGIN      
        1 gtctcactga tgagcgatta ccaaaaccaa agacttactc cttatttata cagaggtagg
       61 gggtgctacg actcgcaggt ccaggctgct gagtatttct ttatggaatc aacatccata
      121 gagtcaattg atgccctcca attaataggg agcgaggaag ttggtgaaaa ccgttgggaa
      181 ataaagttct tatcccgagc tgataaaacg cgctacaaat tcattgtggc tgcagaaaaa
      241 tccgcttttg agatctttga aagctgtagc aaaccagata agagttcatc tcgcctgcaa
      301 taccacctgg tggagtggag tcaagcatga caaacctcac gatctatcaa atttacgatt
      361 tgcttaaccg cccacggcca ctctggctag taaaaatcaa cttgagcgaa acgagtttat
      421 ggatggcaga cttgagcgaa tgcaacttga gttgggctga cctcacccgg tgcgatctga
      481 gttgggcaaa acttattaag actgatctat ccagggccaa tcttagttat gcaaactttt
      541 acgaagctca cctaacccac gccaacctta ctgaagccaa cctgaatcga gcaaacctta
      601 cacgggctaa tttggaaaaa actaacttgt atcatgctgt cgctaccgag gcgaatttta
      661 gccgtgctaa cttaagtcga tccaatttaa aacaagccaa ccttagcggt gcaatattta
      721 gccgggcaga tctaagtaaa tcagatttga tcaactgtaa tatgaacggc gccatcttga
      781 cccatacaaa ccttttcgag gcaaacctgc aaggtgttga tctaaaaggt gctaatctag
      841 agaatgcaga tttgactgca tctaacctgg cagatgccaa tctccatggg gcaaatttga
      901 ccggggctaa cctgacagat gccatactcc cagaaggcat gaaataagac agggtactaa
      961 taatcacttc taattatcat tagcaggaga gttatgtcag ctgatcaaaa tgataagaga
     1021 gttggattcc ttcctttcca cgcgatcaac gaatatatga ccgaggtata tcgcctgcaa
     1081 gtgattcgcg atgtgctgac agggttgccc aatttacctg aggatcaccg aaataagata
     1141 aatcaactga ctagaaagct tgtaaaagtc ccgggcttcc gcaatagcgt gaaagcccca
     1201 ttgcctatac ggataaaatc tactgtccct gcatttgaaa aaaagcctga tttagcagcc
     1261 gctcttttgg cagcctgggc agatgcgaaa gctgagcttc gagagagggt ttatgcactg
     1321 ctcaaatcac gcaattggga attactcccc ctggagacag accgtaccaa gctaccaggt
     1381 tttatgacaa tttggcctgg tggtgaaaat tttgaaattc tcaaccaagc ctttggtgag
     1441 atgtatcctg atttcagtac gacaaatgat gatgtgtcat taatggtggt ttggataagc
     1501 acccggctcc cataccaggt cacggacgag ggagaggtgg aggtgcaaac gataaggtga
     1561 ctctactatc gaccccaacg aggctgccaa tctgagcgca actcacccgt cagacgagtc
     1621 gcatttgaac catcgcttct catcacatac acctcattgt ttccatctcg gtacgatgta
     1681 aacgcaatcc attcaccatc gggggaaaag ctcggaccca ggttatcacc cccatcagtc
     1741 agcttcctaa gttccttgtc atccacattt atcatataaa tgtcgcgatc tccttttggt
     1801 ccagcataaa aagccagcca ttctccatca ggagaccacg tgctacgccc gcctattcca
     1861 gatattccct ttgtgacctg ctttaatttt gtgccatcgg cattgacaat aaataactgt
     1921 gtcgcgccgc ctataatgga ggcaaacgca attctatctc cacttggcga ccaggttgga
     1981 tcaatcccgt aaatatcatc cgaggatatt ggacgagcat tgcctccatc gattttcata
     2041 atccagatcg cctgtttccc gccactctcg gctgtaaata cgatgcgttt tccgtttggt
     2101 gagatctcag gagcatatag attcccgata ttgtcagtta atttgtgaac attgctgcca
     2161 tcaacatcca tgatgtagat ttcaaaattt ccatctcttc ttgacgagaa aacgatccgt
     2221 tccccatcgg gatcgaagga aggataaaaa tttgtgcttt cttcgtcagt caattggata
     2281 atcccgcttc catccgcttt catcttgcag atttgatcat atccatttac aaagcaagca
     2341 tacacgatct taccgggtgg aggttctaac caaaccggag atgaataagt tggtttaggt
     2401 ggaacttgtg tttgcgtagg aaggggttgg ggcgacttgg ttggagaagc ttggggtgtg
     2461 tcaagttgca acgatggtgt gacaagcggg gtaatagttc caaatagctc cgccagcgca
     2521 gtgcgggttg acttcacatc cggaaaggcg tatcttctaa tgagtatccc tgaccccagg
     2581 caaaccatac ataaaattgc tagcaggata gcaaccaaga tatagatcaa tattctgtca
     2641 gaatatctct ttggattgga attgtacggg tccatatgaa tacacacttc gcatacaggc
     2701 aggatgtaat tcccaaatta taacaatact ggaattgcct aaccctttgg gttagaatac
     2761 ccttatgttg gaaattgatg ggtcaatcgg cgaaggcggg ggacaggtac ttcgctctgc
     2821 tctgacactg tcgatattaa ccggaacacc tatgctcatc aaagaaatcc gtgcacgccg
     2881 atcgacacct ggtttgatgc cgcaacatct taaagctgtc gatgctgcag ctgctgttag
     2941 ccgagctcat gtagaggggg ccttcaaagg ttcgacttct ttgaaatttg agccaggcca
     3001 gctgcgatct ggtcgatacc gattcgacat cggtaccgct ggatccacct cgctagtatt
     3061 gcaaaccatt ttcttcctct gagcaaagcc agtagcgcct cttctgtcat catcacaggc
     3121 g
//
LOCUS       DBNHOH010000031         1127 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1475450, whole genome shotgun sequence.
ACCESSION   DBNHOH010000031 DBNHOH010000000
VERSION     DBNHOH010000031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1475450"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="AC16V7_00490"
     CDS             <1..599
                     /locus_tag="AC16V7_00490"
                     /inference="COORDINATES: protein motif:HMM:NF015755.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR6255623.1"
                     /translation="ARTLDLNFGLRVEHYGAVNMDTFVKVVDAVGGIDIYLPNDVDGR
                     PIDDRTEDMGYFTAGQHHFNGNEALRLSRIRKKYNDFTRMDNQNMVLCALKAKITSPA
                     VLPKIPQIIAAFQGAVLTDLSLEQIGQLACLLPRLKRDSLLFTSLPQEILSPGRVFSP
                     QQKKTTFVMEADYEVIRDYVRQFMEGTWPTEPDEPTCP"
     gene            563..>1127
                     /locus_tag="AC16V7_00495"
     CDS             563..>1127
                     /locus_tag="AC16V7_00495"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF012616.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052908 - 16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal RNA small subunit methyltransferase A"
                     /protein_id="HIR6255624.1"
                     /translation="MAHRAGRTDLPVNAPALLRQFGIHPDKSLGQNFLVDESALRKII
                     EIAEISRDEAVLEVGPGLGGLTCQLAAQARHVVAVELDDRLIPILEQELASFSNVQLI
                     QGDILAQNPAELFDERGYLVVANIPYYITSILIRHLLGSSLKPSRLILTVQREVAERI
                     CAQPGELSLLALSVQVYGQPQIRARIPA"
BASE COUNT          252 a          307 c          297 g          271 t
ORIGIN      
        1 tggcaaggac acttgattta aatttcggcc tgcgggtgga acattatggc gcagtaaata
       61 tggatacctt tgtcaaggtt gtggatgctg tcggaggcat tgatatctac ttgcccaacg
      121 atgttgacgg gcgaccgatt gacgatcgca ccgaggacat gggctacttt actgccgggc
      181 agcaccattt caatggtaat gaagccctgc gcttatcacg tattcgtaaa aaatataacg
      241 atttcacccg catggataac caaaacatgg tgctctgcgc tctaaaggcc aagatcacca
      301 gccctgctgt actgccaaag atcccgcaga tcattgccgc ttttcaagga gcagtgctca
      361 ccgatctcag cctggaacag attggccagc tagcctgcct tctgccccgc ctcaagcgcg
      421 atagcctgct tttcaccagc ctgccgcagg aaatcctctc cccgggtcga gtcttcagcc
      481 ctcagcaaaa gaagaccacc ttcgtaatgg aggcggacta cgaagtaatc cgcgattatg
      541 tcaggcaatt catggagggc acttggccca ccgagccgga cgaaccgacc tgcccgtgaa
      601 cgcccccgct ttgcttcgac agtttggcat tcacccggat aaaagcttgg ggcaaaactt
      661 cctggtcgat gaaagcgccc tgcgaaaaat tattgagatt gcggagattt ccagggatga
      721 ggctgtcctg gaggtcgggc ctggtctggg tggcttgacc tgccaattgg cagcccaggc
      781 tcgccatgtg gtggcggttg agcttgatga ccgcttgatc cctattcttg agcaggagct
      841 ggcttccttt tctaatgttc aactgatcca gggcgacatt ctggcacaaa acccggctga
      901 gctctttgac gaaaggggct atctggtagt cgccaatatc ccctactata tcacctcaat
      961 tttgatccgc catttgttgg gatcgtccct taagccatct cgcctgatac tgactgtgca
     1021 gcgggaagtg gcagaacgca tttgtgcaca accgggggag ttgagtctcc tggctcttag
     1081 tgtgcaggtt tatggtcagc ctcagatccg agcacgcatt cccgctg
//
LOCUS       DBNHOH010000032         2448 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1411465, whole genome shotgun sequence.
ACCESSION   DBNHOH010000032 DBNHOH010000000
VERSION     DBNHOH010000032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1411465"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="AC16V7_00500"
     CDS             complement(<1..508)
                     /locus_tag="AC16V7_00500"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR6255625.1"
                     /translation="MNEYEAVIGLETHIQLNTLTKIFCACKADSWNDPPNTNICPVCT
                     GLPGVLPVLNKAVVEKAILLAAAMQAEIQPLSYFDRKNYFYPDLPKGYQISQFDIPLA
                     RGGYLDLPMPEGYTRRVNIHKLHIEEDAGKTKNDDGRRQIDFNRCGVPLVEMVTAPDL
                     RSADEAAQY"
     gene            complement(523..1206)
                     /locus_tag="AC16V7_00505"
     CDS             complement(523..1206)
                     /locus_tag="AC16V7_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HIR6255626.1"
                     /translation="MSKRLSSEEVLARLYSLANPANVTGMARYGINTHNTLGISIYTL
                     RPLAKEIGRDHTLSLELWDSGIHEARILASYIDDPEQVTEEQLEDWVVDFDSWDVCDQ
                     VCNLFAKTPWAYQKAFQWSKREEEFVKRAGFVLMAVLSVHDKQAPDEKLAQFFPVIMR
                     ESTDERNFVKKAVNWALRNIGKRNRHLNELAIETAQQIREIGAKSARWIASDALRELT
                     GEKVQARLR"
     gene            complement(1295..>2448)
                     /locus_tag="AC16V7_00510"
     CDS             complement(1295..>2448)
                     /locus_tag="AC16V7_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR6255627.1"
                     /translation="SNESSAFHPTPRNPWNVQRVPGGSSGGSAAAVAAGEGALSLGTD
                     TAGSIRQPAAFCGVVGMKPTYGRVSRYGLIAFGSSLDCPGPVARNVTDAALMLKVIAG
                     ADRRDATAAKVSVPDYLSDLKRGVRGMRIGLSPDYFRITYFDTSTGELHQEPIAPEIE
                     DALLRAAGRLAEMGADIVEGVPMPHTRYGIPVYFVISRVEAASNLHRYDGVKYGYRTS
                     EPVTDLAEMYRKTRAQGFGPQPKLRILMGMYVSAAQYSEQYYNRALRVRTLIRSDFDQ
                     AFDPQGPYRLQALLTPTTPTTAFPMGAVYGDSVLMQYADQLTVPANHAGAPGLSLPAG
                     FDRDGLPIGIQLLGPDFSEETLLRIGRAYEMATETDEWRGKRPPVLSKI"
BASE COUNT          561 a          687 c          672 g          528 t
ORIGIN      
        1 ggtattgggc ggcctcatca gccgagcgca ggtcgggggc agtgaccatt tcgaccagcg
       61 gtactccgca gcggttgaag tcaatctgcc gtcgcccatc atcgttctta gtcttgccag
      121 cgtcctcctc gatatgcagc ttatggatat tcacgcggcg ggtgtagccc tccggcatgg
      181 gcaagtccag gtaaccgccg cgcgccagag ggatatcaaa ttgcgatatc tggtagccct
      241 tgggaagatc gggatagaaa taatttttgc gatcaaaata cgatagaggc tgaatttcag
      301 cctgcatggc ggcagccagc aagatagcct tctccacaac ggctttgttc aacactggca
      361 gtacccctgg caatcctgta cacaccggac agatattggt attaggcggg tcgttccagg
      421 aatcagcctt gcaggcgcag aagatcttgg tcagcgtatt aagttggata tgagtttcta
      481 acccaatgac ggcttcatac tcattcatga aatgaaccct cgttagcgca acctggcctg
      541 gactttctca ccggtcagct cacgcaatgc gtcggaggcg atccagcggg cagatttggc
      601 accgatctca cggatttgct gggcagtctc gatcgccagc tcgtttagat ggcgattacg
      661 cttgccgata ttacgcaatg cccagttgac cgctttcttg acaaagttgc gctcgtcagt
      721 tgattcacgc atgatgacag gaaagaactg ggctagcttc tcatcggggg cttgtttatc
      781 gtgcacagat aacacagcca tcaggacaaa tccagcgcgc ttgacaaact cctcttcacg
      841 cttgctccat tggaaggctt tctgataagc ccacggtgtt ttcgcaaaca ggttacacac
      901 ctggtcgcac acgtcccagg agtcaaaatc gaccacccaa tcttccaatt gctcctcggt
      961 gacctgctcg ggatcgtcaa tgtaactggc caggatgcgc gcctcgtgaa tacccgaatc
     1021 ccataattca agggaaaggg tgtggtcacg cccgatctct ttggcgagtg gtcgcaaggt
     1081 gtagatagaa atgcccaggg tgttatgggt attgatgcca tagcgcgcca tgccagtcac
     1141 gttggcgggg ttggccaagg aatatagacg ggcaaggact tcttcgctgg atagcctttt
     1201 actcattttg cgcctaaatc aaacaccaag ggcactaatt tctttatctt ccttcacgtc
     1261 ttgtgtgtta gaaattactc cgaacttgcc agaatcagat cttactcagt acaggcggtc
     1321 tcttaccccg ccactcgtcg gtctcagtcg ccatctcgta agcacggcca atccgcaata
     1381 gggtctcctc agagaaatct ggacccagga gctgaatgcc aatgggcaag ccgtcccggt
     1441 caaagcccgc cggtagagat aagccaggtg ccccggcatg gttggcaggc actgttaact
     1501 gatcggcata ctgcatcagc accgagtcgc catacactgc gcccataggg aaagctgtgg
     1561 tcggtgtagt gggggtaagc aaggcttgca agcggtaggg tccctgggga tcaaaggctt
     1621 gatcgaagtc gctacggatg agggtgcgca cacgcagagc ccggttgtag tactgctcgc
     1681 tgtactgggc agcactgaca tacatgccca tcaggatgcg cagtttgggc tgtggaccaa
     1741 agccctgggc acgcgtcttg cgatacattt cagccaaatc ggtcaccggt tcagaggtac
     1801 gatagccgta tttcacgcca tcataacgat gcaggttcga ggctgcctca acgcgcgaga
     1861 taacaaaata gaccggaatg ccgtagcggg tatggggcat gggcacacct tctacgatgt
     1921 cagcacccat ctccgccaat cgaccagcgg cgcgcagcaa cgcatcctca atctcggggg
     1981 caatcggctc ttggtgtaac tcaccagtgc tggtatcgaa ataggtgata cgaaaatagt
     2041 ccggtgacag tcctatacgc ataccgcgca cgccgcgctt aagatcagac aggtagtcgg
     2101 gaaccgatac tttcgctgct gtggcatccc gccggtcggc tccagcgatt accttcaaca
     2161 tcaaggccgc atccgtcacg ttacgcgcca ctggcccggg gcaatccaaa gacgagccga
     2221 aagcgattaa gccatagcgc gagacgcgcc cgtaggtggg cttcatacct accacaccgc
     2281 agaacgcagc cggctggcgt atagagccag ctgtatcagt ccccagagaa agagcaccct
     2341 ccccggctgc taccgccgcg gcgctaccac ccgatgagcc gcccggcacg cgctgtacat
     2401 tccaggggtt gcgcggggtg ggatggaaag ccgacgactc gttggatg
//
LOCUS       DBNHOH010000033         4097 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1347398, whole genome shotgun sequence.
ACCESSION   DBNHOH010000033 DBNHOH010000000
VERSION     DBNHOH010000033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1347398"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..87)
                     /locus_tag="AC16V7_00515"
     CDS             complement(<1..87)
                     /locus_tag="AC16V7_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010879215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR6255628.1"
                     /translation="MQHTLVVLVEDKPGVFSRVASLFRRRAFN"
     gene            complement(102..1820)
                     /gene="ilvB"
                     /locus_tag="AC16V7_00520"
     CDS             complement(102..1820)
                     /gene="ilvB"
                     /locus_tag="AC16V7_00520"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HIR6255629.1"
                     /translation="MKRTGAQIVWETLVREGVDLVFGYPGGANLPIYDAMLGYPVRHV
                     LARHEQGAAHMADGYARASGKVGVAMATSGPGATNLVTGIATAIMDSSPMVCITGQVV
                     SRFIGYDAFQETDVTGITLPITKHNYLVTDVHEIGDVLREAFYIARTGRPGPVLVDIA
                     KDAQQASIEWEYDDSPIRLPGYRPDLRPVTEDIQKALELIYNAKRPVILAGHGVLLSG
                     AMAELREFAERAQTPVAMTLLGLSGMPSDHPLNLGMMGMHGEAWVNHAIQESDLLLAF
                     GMRFDDRVTGNLKTYAPNAKKIHIEIDPSEINKNVTVDVGLIGDLRETLSWILEHLEP
                     CDKSEWLTYIDDMKGDSAVRDIQNLPDSGVLYAAHVINDLWRYTDGKALITTDVGQHQ
                     MWTAQYYKFNTPGNLITSGGLGTMGFGLPSAIGAKFAKPDAQVWTVVGDGGFQMTQAE
                     LATAAQEGVKVNVAIINNGYLGMVRQWQEFFYERRYAATPMRNPDFVKIAEAHGLTGL
                     RVTQRSEVEAAIRSAQEIEGTVVIDFKVEQEDSVYPMVPAGADLHNMIRRPVSNPLVE
                     TAQDTV"
     gene            complement(1893..3596)
                     /gene="ilvD"
                     /locus_tag="AC16V7_00525"
     CDS             complement(1893..3596)
                     /gene="ilvD"
                     /locus_tag="AC16V7_00525"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR6255630.1"
                     /translation="MRSDQIKAGIERAPHRALLKATGVTDADMHKPFIAIVNSYVDVV
                     PGHVHLQGFGKLVKEAVRAAGGVPFEFNTIGVDDGIAMGHIGMHYSLPSRELIADCVE
                     TMIEAHQFDGMVCIPNCDKIVPGMLMAAMRVDIPAIFVSGGPMAAGRTPEGQAVDLIS
                     VFEGVGAYQAGRIDAARLKLLEDFACPSCGSCSGMFTANSMNCLMEALGIALPYNGSA
                     LAQTPEREALASRAAELILELVERDITPRQIVTVEALDDAFALDMAMGGSTNTVLHAL
                     AIANEAGVDYPLSRINHVADQVPHLCKVSPAGKWHMEDVHRAGGIPAILTEIERNSDL
                     LHLDRITVSGKSLRESIAGAAVQDSEVIRPYAEPHSRHGGLAILFGNLAPKGAVVKTG
                     GVSPAMKQFTGRARIYESQENAMTGIMAGQVKPGDVVVIRYEGPKGGPGMVEMLSPTS
                     AIMGMGLGDKVALITDGRFSGGTRGACIGHVSPEAAARGPIAALQQGDVIDIDLEKRT
                     LDVRLTDEEIQRRLESLPAFQSRTRSKWLRRYSYFVSSADTGAVLMDTAEAQTSPSFV
                     R"
     gene            3938..>4097
                     /locus_tag="AC16V7_00530"
     CDS             3938..>4097
                     /locus_tag="AC16V7_00530"
                     /inference="COORDINATES: protein motif:HMM:NF024776.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6255631.1"
                     /translation="MKEQHKLKMVIDYEAGKIRAQRAAELLGITKRQFRRLVAAYRQR
                     GIVGLAHGN"
BASE COUNT          924 a         1235 c         1042 g          896 t
ORIGIN      
        1 attgaacgct cgccggcgaa acagtgatgc gacccggctg aacacaccgg gtttatcctc
       61 tactaagacg actagggtat gctgcatggc tccacctcct cttacactgt gtcctgtgca
      121 gtctcgacca gcgggttaga taccggtctg cgaatcatat tgtgcaggtc agcgcctgcg
      181 ggcaccatcg ggtacacact atcctcctgc tcaaccttaa aatcgatcac cacggtgcct
      241 tctatctctt gcgctgaccg gatcgcagcc tctacttccg agcgctgcgt gaccctcagg
      301 cctgtcaggc cgtgggcttc ggctatcttg acaaaatcgg ggttgcgcat gggggttgcc
      361 gcatagcggc gctcatagaa gaactcctgc cactggcgta ccatccccag atagccgttg
      421 ttgatgatgg caacattcac cttgacgcct tcctgagcgg ctgttgccag ctcggcttgc
      481 gtcatctgga acccaccgtc accaaccacc gtccagacct gtgcatcggg cttggcaaac
      541 ttggcgccga tcgccgaagg caatccaaag cccatggtgc ccaacccgcc cgaggtgatc
      601 agattgcccg gggtgttgaa tttataatat tgcgcggtcc acatctggtg ctgtcccaca
      661 tctgtagtta tcagcgcctt cccatccgta tagcgccaga gatcattaat cacatgggca
      721 gcgtacagga cgccgctatc tggcagattt tggatatcgc gcacagcgct gtcgcctttc
      781 atatcgtcga tataagtcag ccattccgat ttatcgcaag gttcgaggtg ctcgagtatc
      841 cagctcaggg tctcgcgcaa gtcaccgatc aagccgacat caaccgtgac gttcttatta
      901 atttccgacg ggtcaatctc gatatgaatc ttcttcgcat tgggcgcgta cgttttaaga
      961 ttgcccgtca cgcggtcgtc aaagcgcatc ccaaaagcta acaacaggtc tgattcctga
     1021 atagcatggt tgacccatgc ttctccatgc atccccatca tgcccaggtt gagggggtga
     1081 tcagatggca tcccgctcaa tcctaagaga gtcatcgcca ccggggtctg cgcccgttcg
     1141 gcaaactccc gcaactcggc cattgcgccg ctgagcagga caccatgccc ggcgagaata
     1201 accggacgct tggcgttata aatgagctcg agggcttttt ggatatcctc cgtcacagga
     1261 cgcagatcgg gacggtaacc cggcagcctg atcggcgaat cgtcgtactc ccattcaatg
     1321 gaggcctgct gggcatcctt ggcgatgtcc accaatacag ggccggggcg tccggtcctg
     1381 gcgatgtaaa aagcctcgcg caagacatct ccgatttcat gaacatcagt caccagataa
     1441 ttatgcttgg tgatcggcag ggtaatgcca gtgacgtccg tctcctggaa ggcgtcatat
     1501 ccgataaagc ggctgaccac ttgcccggta atgcagacca tgggggacga atccatgata
     1561 gcggtggcaa tgccagtcac caggttagtc gcacccgggc cagaggttgc catcgctacg
     1621 cccaccttcc cactggcacg ggcataccca tcagccatat gcgccgcacc ctgttcgtga
     1681 cgagccagga catggcggac cggataaccc agcatggcat cgtatatcgg caagttcgca
     1741 ccgcctggat atccaaacac caaatccaca ccctctcgca ccagggtctc ccaaacaatt
     1801 tgtgcacctg ttctcttcat tggcctcatt cctttttata aaaccttggc tattccatat
     1861 tcaccaccga aaaatgcaga aaacctagat cgttatcgta caaagctcgg cgaagtttgt
     1921 gcctctgcgg tatccattaa aaccgcgccg gtgtcggcgc ttgagacaaa atacgaatat
     1981 ctacgcagcc acttgctgcg ggttctcgac tggaaggcag gtaagctttc cagccgtcgc
     2041 tggatttcct cgtcagtgag gcgtacatcc agcgtgcgct tctccaggtc aatatcgata
     2101 acatctccct gttggagcgc agcaatcggg ccgcgagcag ccgcttcagg tgagacatgc
     2161 ccgatgcacg cgccccgtgt cccaccagaa aagcgcccat cggtgatcaa agctacctta
     2221 tcccccaagc ccatacccat gatggcgctg gtcgggctga gcatctccac catccctggc
     2281 ccacccttcg ggccttcata gcggatgaca accacatcgc caggtttgac ctgccctgcc
     2341 ataatgcccg tcatcgcatt ttcctgggat tcatagatgc gagcccggcc tgtaaattgc
     2401 ttcatcgctg ggctgacgcc accggtcttg accaccgcac ccttcggcgc caggttgcca
     2461 aacaagatgg ctagcccacc atggcgggag tgtggctctg cataggggcg gatgacttcg
     2521 ctgtcttgaa ctgcagcacc ggcaatcgac tcacgcaagc tctttccaga gacagtaata
     2581 cgatctaggt gcaacaggtc agagttgcgt tcaatttcag tcaagatggc aggtataccc
     2641 ccagcccggt gcacatcctc catgtgccat ttgcccgctg ggctgacctt gcacaaatgc
     2701 ggcacctgat ctgccacatg attaatacgg cttagcgggt aatccacacc ggcttcattg
     2761 gcaattgcca gcgcatgcaa aaccgtattc gttgagccgc ccatagccat atccagggca
     2821 aatgcgtcat ccagcgcctc aacggtaaca atctggcgcg gggtgatgtc ccgctcgacc
     2881 agctccagga tgagttcggc agcccggctt gccagcgctt cacgctcggg tgtctgcgcc
     2941 agcgccgaac cgttgtaggg caaggcgata cctagggctt ccatcaaaca attcatgctg
     3001 ttggctgtga acatgcctga gcacgagcca caggagggac aggcaaaatc ctccagcagc
     3061 ttcaagcgag cagcgtcgat gcgacctgcc tggtacgccc caactccctc gaaaaccgag
     3121 atcaggtcca cagcctgacc ttccggtgtt cgcccggctg ccattggccc gcccgagacg
     3181 aaaatggccg ggatatccac gcgcatcgcc gccatcagca tccccggcac gatcttatcg
     3241 cagttgggaa tgcacaccat gccatcaaac tggtgggctt cgatcatcgt ctcgacgcaa
     3301 tctgcgatca gctcgcggga tggcaaggaa tagtgcatgc ctatatgccc catggcgatg
     3361 ccatcgtcca cgccaatcgt attgaattcg aacggcactc cacccgccgc cctcaccgcc
     3421 tctttgacca gcttgccaaa gccctgcaag tggacatgcc ccggcaccac atcaacgtat
     3481 gaattgacaa tcgcaatgaa gggtttgtgc atatccgcat cagtgacacc tgttgctttg
     3541 agcagcgcac ggtgcggggc cctttcaatc ccggctttga tctggtctga tctcacataa
     3601 cctccttgat aaaaaaaacc gccccgttgg gggcggttcc gctttacaac ttttggttta
     3661 cctactttga cctgcgctgt ttcctgcggg tcaccctggc aaccgcccaa accggaatat
     3721 ggtccttaat aataaggacc gaaaggagga cgataataac cagggatagg attatgttca
     3781 ttagattaag actttctagg gttaatatgc tggattatac gacattatcc gagattgtca
     3841 agtgccaatt ctcgaaattg ttgttgttca gcgatattga caccccctga ctgttcaggg
     3901 aaaatgtcac cccatggaaa acaaggtgac attaacgatg aaagaacagc acaaactgaa
     3961 aatggtgatt gactatgaag ccgggaagat acgagcccag agagcggctg aactgcttgg
     4021 catcacgaag cggcagtttc gccggttagt agcggcctat cgccagcgag ggatagtcgg
     4081 cctggcgcac gggaacc
//
LOCUS       DBNHOH010000034         2265 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_706783, whole genome shotgun sequence.
ACCESSION   DBNHOH010000034 DBNHOH010000000
VERSION     DBNHOH010000034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_706783"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /gene="ilvN"
                     /locus_tag="AC16V7_00535"
     CDS             <1..321
                     /gene="ilvN"
                     /locus_tag="AC16V7_00535"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HIR6255632.1"
                     /translation="QVHDMTTVPTVSRDLAMIKVNAPAENRTHIMQTVDVFRARILDV
                     TNNSFIIEVTGDEEKINGFVEVLKPLGIIEMVRTGKVTMARGATPLPDANGNGHRPAA
                     EMMV"
     gene            382..1434
                     /gene="ilvC"
                     /locus_tag="AC16V7_00540"
     CDS             382..1434
                     /gene="ilvC"
                     /locus_tag="AC16V7_00540"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIR6255633.1"
                     /translation="MAWMDFCGTQEEVVTREEFPLEKAREVLKDEVIAVLGYGVQGPA
                     QALNMRDNGFNVIVGQKPGGKSWEKAVKDGWVPGETLFSLEEATERGTVIQYLISDAG
                     QRLMWPKIKPCLNEGDALYFSHGFSVVYQDQTGIVAPEYVDVILVAPKGSGASVRTNF
                     LNGSGINSSFAVEQDYTGRARERTLALGIAIGSGYLFATTFKHEVYSDLTGERGVLMG
                     ALAGIMEAQYQVLREHGHTPSEAFNETVEELTQSLIRLVAQNGMDWMYANCSTTAQRG
                     ALDWRHQFKDAVDPVFQRLYESVAAGEETRIVLNSNSAPDYKEKLDAELQEMRNSELW
                     QAGALVRSLRPENWQQ"
     gene            1546..>2265
                     /locus_tag="AC16V7_00545"
     CDS             1546..>2265
                     /locus_tag="AC16V7_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/alpha barrel domain-containing protein"
                     /protein_id="HIR6255634.1"
                     /translation="MTDNYVRFFDTTLRDGEQSPGATMTSAEKLEVARALARLGVDII
                     EAGFPAASPDDLEAVRRIAIEVGNPSDGTRPPVICGLARTTRGDIDKAWEAIRHAAHP
                     RIHTFLATSDIHMKHKLKMTRRQVVERVGEMVAYARSLCEDVEFSPEDAGRSDPEFLY
                     QVLEVAIKAGATTLNIPDTVGYTLPEEFGALIAGIIQNTPGIQNCIVSVHCHNDLGMA
                     TANTLAGIRAGARQAEVTINGI"
BASE COUNT          542 a          560 c          692 g          471 t
ORIGIN      
        1 caggtgcacg atatgaccac agtccccacg gtcagccgcg acctggcgat gatcaaggtc
       61 aatgcccctg cggagaaccg tactcacatt atgcaaacgg tggatgtatt ccgggcgcgc
      121 attttggatg tcaccaacaa ttcgttcatc atcgaggtga cgggagatga agaaaagatc
      181 aacggcttcg tggaggtatt gaaaccctta gggattattg aaatggtacg gaccggtaaa
      241 gtgaccatgg cgcgcggcgc cacgccgctg ccggacgcca atgggaatgg acatcgccct
      301 gcggctgaga tgatggtata gcagaggtaa ttgttgggtc agattgaccc ttaattcaca
      361 cactagataa ggagcaatgg aatggcctgg atggattttt gcggtacaca agaggaagtg
      421 gttacgcgtg aggaattccc gttggagaaa gcgcgggagg tacttaagga cgaagtgatc
      481 gcggtgttgg ggtatggcgt tcagggacct gcgcaggcat tgaacatgcg cgataacgga
      541 tttaacgtga ttgtagggca aaaacccggc ggtaaatctt gggaaaaagc ggttaaggat
      601 ggttgggtcc cgggagagac cctgttttca ctcgaggaag ctaccgaacg cggcacggtg
      661 atccagtatt tgatctccga tgccgggcag cggctgatgt ggccgaagat caagccctgc
      721 ctgaacgaag gagatgcatt gtatttctcg catggcttct cggtcgtata tcaagaccaa
      781 accgggatcg tagctccaga atatgtggat gtcatcctgg tggctcccaa aggctcggga
      841 gccagtgtgc ggactaattt cctgaatggc agcgggatca acagcagctt cgcggtggag
      901 caggactaca ccggtcgcgc acgagaacgc accctggcct tgggaattgc gattggttcg
      961 ggttacctgt ttgccaccac gttcaagcat gaggtctaca gcgacctgac cggtgaacgc
     1021 ggcgtgttga tgggcgcgtt ggcgggcatc atggaagccc agtaccaggt gctgcgcgag
     1081 catggacaca cgcccagcga ggcattcaat gagaccgtcg aggagctgac tcaaagcctg
     1141 atccgcttgg tggcacagaa tggtatggac tggatgtatg ccaattgcag caccactgcg
     1201 caacgcggcg cgctagactg gcgtcaccag ttcaaagatg ctgtggatcc ggtattccaa
     1261 cggctgtacg agagtgtcgc tgcaggcgag gagacgcgca ttgtcttgaa ctccaacagc
     1321 gccccggatt acaaagagaa gctggatgcc gaattgcaag agatgcgtaa ttcggagctt
     1381 tggcaggcgg gtgcattggt gcgctccctg cgacctgaaa actggcagca atgattatca
     1441 ctttagccat ttgaaactat gaagccaaag gttaagtgag tcagcgagat catagcttgt
     1501 tgtagagaga actgttttac tggtattaca agcgcggagg aatagatgac agacaattat
     1561 gtacgattct tcgatacaac tttaagggat ggtgagcaat cgcctggtgc aaccatgacc
     1621 agcgccgaga agctggaggt cgctcgcgcc ttggccaggc tcggtgtgga tatcatcgag
     1681 gcaggttttc cggctgcctc tccagacgat ctggaagcgg tgcgccggat tgctatcgag
     1741 gtaggcaatc ccagcgacgg cacccgcccc ccggtcatct gtggacttgc gcggaccacc
     1801 cggggtgaca tcgataaggc ctgggaagca atccgccacg ctgcccaccc caggattcac
     1861 accttcctgg caacgtcaga tatccacatg aagcacaagc ttaagatgac tcgccggcaa
     1921 gtggttgagc gggtgggcga gatggtcgcc tacgctcgca gcctgtgcga ggatgttgaa
     1981 ttcagccccg aagatgccgg tcgcagcgat ccggagttcc tgtatcaggt gctcgaagtg
     2041 gctatcaaag ctggcgcgac aacgttgaat atcccggata cggtcggata cacgttgcca
     2101 gaagaatttg gcgctttgat cgccggcatt atccaaaaca cccctggcat ccaaaactgc
     2161 attgtttctg tccactgcca caacgatctg gggatggcaa ccgcaaatac tttggcaggt
     2221 atccgtgctg gagcgcgcca ggctgaagtg acgatcaacg ggatc
//
LOCUS       DBNHOH010000035         1495 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_642553, whole genome shotgun sequence.
ACCESSION   DBNHOH010000035 DBNHOH010000000
VERSION     DBNHOH010000035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1495)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_642553"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..970
                     /locus_tag="AC16V7_00550"
     CDS             263..970
                     /locus_tag="AC16V7_00550"
                     /inference="COORDINATES: protein motif:HMM:NF015534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HIR6255635.1"
                     /translation="MLALVGSGEYLPDMETVDRFLLDELGTAPRVVCLPTAAGSEGPQ
                     RIAYWSELGVNYFARLGASAEALPVITRQDAQNPILADRVKESNFIYLSGGRPDYLLK
                     TLQDSLVWQAIQDVLMRGGILAGCSAGAMVLGRWIPGFPGMKPAFNLLEEAIVLPHFD
                     EIPNWMVRLILIWVVRRGRMVGVPGFTALVVKGDACKVVGKGNVTIWDSSGKQDYTPG
                     DLLAWSTRTTMGQNQPD"
     gene            1161..>1495
                     /locus_tag="AC16V7_00555"
     CDS             1161..>1495
                     /locus_tag="AC16V7_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006513406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HIR6255636.1"
                     /translation="MTSITASDLLTIIFVLVDDWYQAQGIRYLKGKPGAKPEFSDSEV
                     ITLLLAMDFIPFPSETQFLGFIRANYRALFPRLIDQSQFNRRARALRLLVEELRRAWL
                     VQLGVFHHT"
BASE COUNT          368 a          348 c          387 g          392 t
ORIGIN      
        1 catgccgcag ccaggatttg acctggtttg tcgctatttt ggagctcaaa attcaacctt
       61 tacctttcgg attttggtta aagagccaaa gtccagctca ttttctctcc tcagtggtgg
      121 gaaatcgccc aaatgcgatt gccctagccg cgtaactact ggagttgtaa tttggaaaga
      181 tatccgctaa aattcccctt ttcatgtatg ggatggcgtc ttcctggcac aggtcgagcc
      241 gttaacaaac ccgaggtgaa caatgctggc attggtaggc agtggagaat atttgccgga
      301 tatggaaacc gtcgatcgat tcttattgga tgagcttggc acagcaccca gggtggtctg
      361 ccttcccaca gctgccggca gcgaaggacc gcagcgtatc gcctattggt cagagttggg
      421 agtgaactac tttgcaaggc tcggagcaag cgcagaggca ttgccagtca ttacccgcca
      481 ggatgcccaa aaccccatcc ttgcagatcg cgtgaaggaa tccaatttta tctacctgtc
      541 tggcggcagg ccggattatc tcttaaaaac cctgcaggac agcctggttt ggcaagctat
      601 ccaagacgtg cttatgcgtg gcggcatact ggctggatgt agcgcagggg cgatggtgct
      661 ggggagatgg atccccggtt ttccaggtat gaagcctgcc ttcaacttgt tggaagaggc
      721 gatcgttctg ccacactttg atgagatacc aaattggatg gttcgcttga tcctaatctg
      781 ggtagtccgg cgagggagaa tggttggcgt gcccggcttt accgctttgg tggtgaaagg
      841 ggacgcttgc aaagtagtag ggaaaggtaa tgtgaccatt tgggatagct ctggaaagca
      901 agactacact cccggagatt tgctggcttg gagtacaaga acaactatgg gtcaaaatca
      961 acctgattga tgtctggcac ttgtaggctc cctgaatgca gggtaataca cttgttacga
     1021 gcagcattta aattccaaca atttcttaat ctaaaatcgg ataagattaa tcacgtctga
     1081 tgtgaaatca tctccatttg acaaaaatgg gaacatcctt gaagattcga gtgtcaacca
     1141 aacttcacag gaggttcccg atgacaagta taactgcttc cgatcttcta acaatcattt
     1201 tcgtcctggt cgatgattgg tatcaagcac aaggcattcg atatctcaaa ggtaagcctg
     1261 gtgccaaacc tgagttcagt gatagtgaag tgatcacctt gttattagcg atggatttca
     1321 ttccttttcc cagcgagacc caatttctgg gtttcatccg agccaattac cgggcactct
     1381 tccccaggct gatcgatcag agccaattca accgtcgggc aagagctttg cgcctgttgg
     1441 tagaagagct acggcgtgcc tggttggtcc aattgggtgt tttccatcac accca
//
LOCUS       DBNHOH010000036         2727 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_770629, whole genome shotgun sequence.
ACCESSION   DBNHOH010000036 DBNHOH010000000
VERSION     DBNHOH010000036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_770629"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="AC16V7_00560"
     CDS             complement(<1..599)
                     /locus_tag="AC16V7_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR6255637.1"
                     /translation="MNTTMKTRPPINWGRVASYAALVLFAVIYAGPLLMLVNTSLKTL
                     PAFMKDATSLVTSLHFDNFIEAWEKANFPRYLTNSLIYTLSATLIYVVTAIFVAFPVA
                     RGYVRGANFILTMFVIALFLPPALIPQFQLMLRLGLYNNPVGYILLFTINPIGMVILV
                     NYIKSIPKELDEAAALDGCGYFRFVTAVLFPLIRPAVATV"
     gene            complement(596..1525)
                     /locus_tag="AC16V7_00565"
     CDS             complement(596..1525)
                     /locus_tag="AC16V7_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018885081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR6255638.1"
                     /translation="MMAYHFGRGRARLSPYLLLIPGILLYLLIALGPSLATSVFSFTD
                     ATGIRGAPIHWIGLENYREFLLLGQASRDNYAALGRTLIFCFFVTTIQFGLGLLVAML
                     VNQRLKGTNFFRTLYFMPVILGAVIQGLIWSLFLYPLGGPMARILEFFGTSSEFFGGQ
                     PNEAFAYVIVVQIWANMGTTMVIFLAGLQTIPDELYEAAQIDGASGWQVFSNVTWPLL
                     TPSVNTNLLLNIIGSLQAWQLFLVLIGYRNGTQVLGYLVYAQGFGQTSGSMVNPFRQG
                     YAAAASIVLFFLVLVLGMVTQTFLRRREERILG"
     gene            complement(1605..>2727)
                     /locus_tag="AC16V7_00570"
     CDS             complement(1605..>2727)
                     /locus_tag="AC16V7_00570"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6255639.1"
                     /translation="VTVDMSVVNANDLATVTQTRLTANDVDVVDMFAFTNAAQPYMKN
                     VEPPNWQTLIEAGYLLDISGQPFLDNYFPASIADAGTYNGKVYQINLGTVSYSGIYYN
                     ESLFAEYNVAVPTTWDELVAACETFTAADIPCMTAGGKDGWPIFVGAYGLIGSIYPDQ
                     AALVEGLWTGSIKWNDAKSMEMWDKMKVYAQNMMEAGASGIAGDAAPGRFASGAVAMF
                     SGGTWYAAAIEEAEPDFEWGYIPFPGSDNPEDNKYLFGKYDQGWVIAANSPNTDTGLL
                     YLEEFSDPANYQEFVNAVGFFPTQPGATLDTQIGQEVAPYLENFRVGYEQYWVAPKGA
                     GQYANPWASYFQPFGEFDDAQELADKVQADLQSGLDASQ"
BASE COUNT          737 a          686 c          715 g          589 t
ORIGIN      
        1 accgtggcta cagctggccg aatcagggga aagagaaccg ccgtaacaaa gcgaaagtac
       61 ccacaaccgt ccaaggcagc tgcctcgtcc agctccttag gaatactctt gatgtagttc
      121 accaagatca ccatgccaat ggggttgatg gtaaacagca agatgtatcc cacgggattg
      181 ttatatagac ctagacgtaa catcaactgg aactggggaa tcaaggccgg gggcaggaac
      241 aaggcgatca cgaacatggt taagataaaa ttggcacctc tgacataacc gcgagcaacc
      301 gggaaagcga caaatatcgc cgtaaccaca taaattaatg tggctgagag ggtatagatc
      361 agcgagttgg tcagatagcg agggaagttg gctttttccc aggcttcgat aaagttatcg
      421 aaatgcaagc ttgtgaccag ggaggtggcg tccttcatga acgctggtag cgtttttaag
      481 gaagtattta ccagcatgag caagggtccc gcataaatga ccgcaaatag cactagggct
      541 gcataggagg cgacacgacc ccaattgatc ggtgggcggg ttttcatggt tgtgttcatc
      601 ccaaaatcct ctcctctctg cgacgcagaa acgtttgggt taccattccc agaaccagta
      661 ccaggaaaaa cagcacgatg gatgctgctg cagcatatcc ttgccggaac gggttgacca
      721 tgctacctga ggtttgacca aaaccttggg cgtataccaa ataacccaag acttgggtcc
      781 cgttccggta accgatcaaa accaggaata actgccacgc ctggagcgaa ccaatgatat
      841 ttaaaagcag attggtgttc acactcggtg taagcagagg ccaggtcacg ttcgaaaaga
      901 cttgccagcc gctagcacca tcgatctggg cagcttcgta gagttcgtca ggaatggttt
      961 gcaggcccgc caggaaaatg accatcgtgg tgcccatatt agcccaaatc tggacgacga
     1021 tgacataggc aaaggcttcg ttaggttgtc ctccaaaaaa ctctgaactt gtcccaaaga
     1081 attccaggat gcgcgccatc gggccgccta atgggtagag gaaaagcgac cagattaagc
     1141 cctggatgac tgcacccaag atcactggca tgaagtacag cgtgcgaaaa aagtttgtac
     1201 ctttcaagcg ctggttgacc agcatcgcta ccagcaagcc cagtccgaac tggatagtgg
     1261 tcacaaagaa acagaaaatc agtgtccgac caagggcagc atagttatcc cgagaagcct
     1321 gacccagtaa aaggaattct cggtaatttt ccagcccgat ccaatggata ggcgcgccgc
     1381 gtataccggt cgcatctgtg aaggagaaga cgctcgttgc cagagacgga cccaaggcga
     1441 taagaaggta aaggagtatg ccggggatga gcaataagta aggagataac ctagcccgtc
     1501 cgcggccgaa gtgataagcc atcatggtat gcctcctcgt atagattaga gaaaagtagg
     1561 cttctcatca cagacgagaa gcctactgga gaagaagcta ttctttactg gctggcgtcc
     1621 aggccagatt gaaggtctgc ctgaactttg tctgccaatt cctgcgcatc atcaaattcg
     1681 ccaaatggtt ggaaatagga agcccacggg ttcgcatact ggccagcgcc ctttggtgcg
     1741 acccaatact gctcataccc aacccgaaaa ttttccaggt aaggggctac ttcttgaccg
     1801 atctgggtat ccaatgtagc gcccggctgg gtggggaaga aaccaaccgc gttgacaaat
     1861 tcctggtagt ttgctggatc agaaaattct tccaagtaca ggaggccggt atcggtattg
     1921 ggggaattgg ctgcgatgac ccagccctga tcgtacttcc caaacaggta tttgttgtct
     1981 tcggggttgt cgctgcccgg gaaggggata taaccccact cgaaatccgg ttctgcttct
     2041 tctattgcgg cggcatacca ggttccacct gagaacatgg ctactgcgcc ggaggcaaaa
     2101 cgtccgggag cagcatcacc ggcaataccg ctagcaccgg cttccatcat gttctgggcg
     2161 tagaccttca tcttatccca catttccatc gacttggcgt cattccactt gatcgagcca
     2221 gtccagagac cctcgaccag ggctgcctgg tccggataga tcgagccaat caggccgtat
     2281 gcgcccacga agatgggcca gccgtcttta ccgccagcgg tcatgcaggg gatgtcagca
     2341 gcagtaaatg tttcacaggc tgcaaccagc tcgtcccagg ttgtggggac ggctacatta
     2401 tattcagcaa aaagagactc gttgtaatag atgccactgt aggacacagt ccccagattg
     2461 atctgatata ccttgccgtt gtaggtgcct gcgtctgcaa tggaagccgg gaagtaatta
     2521 tccaaaaacg gctgacctga gatatcaagc aggtaaccag cctcaatcaa tgtttgccag
     2581 ttcggtggct caacgttttt catataaggc tgagccgcat tcgtaaatgc aaacatatcg
     2641 accacatcta cgtcattggc ggttaagcgg gtttgggtaa ctgtggccaa atcattggcg
     2701 ttcacaaccg acatgtctac agttacg
//
LOCUS       DBNHOH010000037         1149 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1668179, whole genome shotgun sequence.
ACCESSION   DBNHOH010000037 DBNHOH010000000
VERSION     DBNHOH010000037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1668179"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="AC16V7_00575"
     CDS             complement(<1..414)
                     /locus_tag="AC16V7_00575"
                     /inference="COORDINATES: protein motif:HMM:NF014750.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6255640.1"
                     /translation="MAYRSTNAIINLTTGDVKITRTPEALEQAYLGGRGLNMAFLRHY
                     LRAAGEPLQIDPLSPENPLIFGAGMLTGTIAPNAARFNVSARSPESGILGDANCGGFF
                     AAAMRKAGFDRLVILGKAERPTYILLEDGVVHILDA"
     gene            complement(506..766)
                     /locus_tag="AC16V7_00580"
     CDS             complement(506..766)
                     /locus_tag="AC16V7_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR6255641.1"
                     /translation="MLIYVRLAEPYWRTVGQRDLELELEDQARVSDLLALLTKRHPAL
                     KQELVQAPPHLFIGELEVSEQSPLEEGDRLYVVWPIAGGTQG"
     gene            complement(772..>1149)
                     /locus_tag="AC16V7_00585"
     CDS             complement(772..>1149)
                     /locus_tag="AC16V7_00585"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HIR6255642.1"
                     /translation="CGRTIARMEKIRGRTDDMLIVRGVNVFPSLVEKALLSVEGLDPY
                     YQIVVDRAKDQLDKLEILVEANRRYFEPIDTRALDQLRDAAEQELSQTLGVGAVVKLM
                     GPHSIERSEGKAKRVVDKRDLYK"
BASE COUNT          240 a          330 c          323 g          256 t
ORIGIN      
        1 ggcgtccaga atgtgcacga ccccatcttc caggagaatg taggtaggtc gctctgcttt
       61 ccctaagatc accaggcggt caaatccggc tttgcgcatt gccgccgcaa aaaaaccgcc
      121 gcaattagca tctcccaata tgccgctttc cggtgagcgt gcagatacgt tgaagcgcgc
      181 tgcattcggg gcgatcgtgc cagtcagcat acctgcgccg aagatgagcg gattctctgg
      241 gctgagcggg tctatctgga gcggctcgcc tgccgcccgc aagtagtggc gcagaaatgc
      301 catgttcaag cctctcccgc ctaggtaagc ttgttctagc gcttctgggg tgcgggtgat
      361 tttcacatca cccgtagtca ggttgatgat cgcattggtg gaacgataag ccacaatacc
      421 tcctaaagat accggatttg ggacggtctc acctcattgc agagatgcag cgcagcatga
      481 gcgtaaactg gctttgttta tatacctatc cttgcgtccc tccggcgatg ggccaaacca
      541 catataaacg atcgccttct tccaaagggg attgctcgct gacctccagc tcgccgatga
      601 acaggtgagg cggtgcctgc acaagttctt gcttaagagc aggatggcgc ttggtgagca
      661 gggccaggag atctgaaacg cgggcttgat cttcgagttc cagctccaga tctcgctggc
      721 ccacagttcg ccagtatggt tcggcgaggc gcacgtagat cagcataacg gttacttgta
      781 caagtcgcgc ttgtcaacca cgcgcttggc cttgccctca gaacgctcaa tcgagtgtgg
      841 gcccatcagc ttgacaactg cgccaacccc cagggtctga gagagctctt gctcggcggc
      901 gtcgcgcaat tgatccaggg cgcgggtgtc gatcggttcg aaatagcggc gattggcctc
      961 caccaaaatt tccagcttat cgagctggtc cttggcgcgg tcgaccacga tctggtaata
     1021 aggatccaag ccttctacac tcagcagggc tttctccacc agggatggaa atacatttac
     1081 gccgcgcacg atgagcatgt catccgtgcg gccgcgaatc ttttccatgc gggcgatggt
     1141 gcgcccgca
//
LOCUS       DBNHOH010000038         2273 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1732205, whole genome shotgun sequence.
ACCESSION   DBNHOH010000038 DBNHOH010000000
VERSION     DBNHOH010000038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1732205"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="AC16V7_00590"
     CDS             complement(<1..464)
                     /locus_tag="AC16V7_00590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR6255643.1"
                     /translation="MTLNAHPELPEFDYIRPASLMEASRFLAQHPGEARPLLGGTDIF
                     VRMRDGFWHDKYLVDVKNLDSTHDLHFDPASGLTIGAGVNMNRVIASPEVQAHYPLLA
                     EACRSVASYQLRTRATIVGNICNASPAGDTIGACLLLDGALKVHGLDGLREVP"
     gene            949..1833
                     /locus_tag="AC16V7_00595"
     CDS             949..1833
                     /locus_tag="AC16V7_00595"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022834.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxamate carbamoyltransferase subunit AllH family
                     protein"
                     /protein_id="HIR6255644.1"
                     /translation="MSQKTITISEIGAIARGLLAQESNASVMGVTSRGLFLLLNSGWV
                     VFLSPEAYRGPLTLNYRSDTDPFRLLQTKQSARIAAGSISIPDAGLLINTGQAATWES
                     PSIPGGILPPEQRAAQLVNIARHLLGIKQTSQLSELLPLLLGWVDRAEFQDNSLLPLL
                     DDVKRSLHRRQATEIAHSLVALLGMGSGLTPSGDDVAIGFLLAMARWGHELAPDLDIG
                     ALGQMILPQAYRKTTTLSANMIDCALRGQANERLLLALDGILSGNADASACSSCLDGW
                     GNTSGLDALLGISLAIGV"
     gene            complement(1919..>2273)
                     /locus_tag="AC16V7_00600"
     CDS             complement(1919..>2273)
                     /locus_tag="AC16V7_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255645.1"
                     /translation="FRDCKDLLHLPKLMNKLQHHLEQMIALTLIAFTIGLWFGEALRD
                     VTYAKLKPAQLPNTLGSTMAIDTHQYPKWLLYSGLFVLLKQKLRISQPELDQVALVAA
                     RAFASLIYGNVRSFV"
BASE COUNT          552 a          546 c          615 g          560 t
ORIGIN      
        1 gggacctcgc gcaagccatc gaggccatgt actttcagtg ccccgtctag taacaagcat
       61 gcgccgatcg tgtccccagc aggtgacgcg ttgcagatat tgcccacgat agtagcccga
      121 gtgcgcaatt ggtaagatgc tactgagcgg caggcttcgg cgagtaacgg gtagtgtgct
      181 tgaacctcgg gggaagcgat cacccggttc atattaaccc cagcgccgat ggtcaaccct
      241 gaagcggggt caaagtgaag atcgtgcgtg ctatccaagt tcttaacatc gaccaggtat
      301 ttatcgtgcc agaatccatc tcgcatgcgg acgaatatgt ctgtgccgcc taaaagcggg
      361 cgcgcttcgc caggatgctg ggctaagaaa cggcttgctt ccatcaaaga tgcaggccgg
      421 atatagtcga attcaggaag ttctgggtga gcattaaggg tcatctttca cacctcatct
      481 atttagggac tgtgattcta tcaatggctg ggtcattctt cgtggctggc tgcgggcaaa
      541 atcatgaaat ccagttcaat ttgctaggtg ggatggaatg cgattttagg ttggaaggct
      601 catttattta attaactcag gacctcctct gccaatggta aaacacccta tatctgtcaa
      661 agttaccacg ggaaacaaaa tgagcatcca tagtctaata gaaaattggt ggtatggaca
      721 gtaaagcacg cccataccac ctagttagat cccaaccgcg taaacactgt tattattatc
      781 gtggcaattg atatgccgtt caactggtag atgtcatctt ctcgatatga tatttgtcaa
      841 ggaggatctc ttcgataaag agtacgttaa gagtaataga tttgggatgc tcattgatat
      901 attcggaact caggcgcaag ctaacggtta attaaaacgc gctcagttat gtctcaaaaa
      961 acaatcacca tcagtgaaat cggagccatt gcccgaggat tgctggctca agagagcaat
     1021 gccagtgtga tgggtgtcac ctcgcgtggc ttgttcctgc tgctaaattc aggatgggtg
     1081 gttttcctat cgcctgaggc ttaccgagga ccactgactc tcaactatcg tagtgataca
     1141 gaccctttta gattgctgca gaccaagcaa tcagccagaa tagctgcagg cagtatctct
     1201 attccagatg caggcttgct gataaatacc gggcaggctg ccacctggga atcgccttcc
     1261 ataccggggg gtatcctgcc tcccgagcaa cgcgccgctc agctagtgaa catcgcccgc
     1321 cacttgcttg gcataaaaca gaccagccag ctcagtgagt tattgccact tcttttggga
     1381 tgggttgata gagcggaatt ccaagataat tcgctcttac cgctcctgga tgatgtgaag
     1441 cgttcgctcc atcgtcgcca agcaactgag atagcgcata gcctggtggc tttgctgggg
     1501 atgggtagtg gtctgacccc ctcgggggat gatgtagcga tcggcttttt gctagcgatg
     1561 gcacgctggg ggcatgagct cgctcctgat ctggacattg gtgccctcgg tcaaatgatc
     1621 ctgccgcagg cgtaccgaaa gactacgacg ctgtcagcca acatgatcga ttgtgccttg
     1681 cggggtcagg caaatgagcg cctgctgctg gcgctggatg gcatcttgag cggtaatgca
     1741 gatgcatccg cctgctcttc ttgtctggat ggatggggga atacttcagg gctggacgcc
     1801 ctgttgggaa tctccctggc tattggggtt tagcgtattc cctagccaca caagtcgtca
     1861 cgggtgaatc tgcggttcgt gttgaactcg ctggtcctct gtgagccaaa agtgaggttc
     1921 agacgaaaga tcggacattg ccgtagatga gcgaagcgaa agccctggca gcaaccaaag
     1981 ccacttggtc caattctggc tgggagatac gcaacttctg tttgagcagc acaaatagac
     2041 cagaatacaa cagccacttg ggatattgat gggtatcgat cgccattgtg cttcctaagg
     2101 tattggggag ctgagccggt ttcagcttag cgtaagtcac atcacgcagc gcctctccaa
     2161 accaaaggcc gatggtaaag gcgatcaagg taagcgcaat catctgttcc aggtggtgct
     2221 ggagtttatt catcagcttg ggtaaatgga gcaaatcttt gcaatcgcgg aaa
//
LOCUS       DBNHOH010000039         3972 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_642631, whole genome shotgun sequence.
ACCESSION   DBNHOH010000039 DBNHOH010000000
VERSION     DBNHOH010000039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_642631"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(374..2728)
                     /locus_tag="AC16V7_00605"
     CDS             complement(374..2728)
                     /locus_tag="AC16V7_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HIR6255646.1"
                     /translation="MKQKHLIFGILILGLVLVLSACAGPAGPPGPQGPEGPAGPAGSA
                     GEPGAPAEVSVAEPELVVQPETCAICHKGAGDKHQASYDELYQDGVIQVTDLAYRFSA
                     PDKSVVTFKMTKNGAPFNANDADNLGIYFAPYTGTNFQFEPAAGRLSLKGDITCDSAG
                     ACTSTLVGDAPNVGSTPGLVVLYGRDEDVGRLPARIAQTKYPFAAILETGSGVDYVSA
                     ANNAGCEKCHTDPYLKHGYIYAQLNQDPATDFYTCKACHLDNGEGGHLEWQLLVDNPE
                     LAAGYFAGDVELTDEQKAQYAYNTSLMNDVHMSHAMEFPYPQSMSNCVTCHEGKLDLV
                     LSDANFNITTCKSCHPVTGTVGPVKEGQEEPSYDTTTLALKTILPPAIHGSLDLDTAD
                     CLSCHGEGKAAPPFNQIHTGYNDVIYTADGLKYSEAISVTVDGASFDGSMLNIQFGAV
                     ETQDLEGLDVADIAPTVMVGLYGWDTKDFIIGPHERLFDDNGDGTIDRSDGRALEYGV
                     GEENPRFTTVSAGSGKWEVTADLSAWADMIADGTVMRVEIGVMPTLENADGVVLALNA
                     PSRTFDLGANAFVDDFYGPLVKVVDGCNNCHEALATTFHSPDRGGNVVVCRMCHITKS
                     GGSHLEMQSRSIDSYVHAIHSFQAFDIGDIDFADPVEAMHYEHHIEFPYPKHGITDCS
                     SCHVDGTNNVPDQSKSLPGLLSASDPVNGWDRKIGEVPEYVTGPASRACGGCHRAVMI
                     KEDEAVELTAFNLHTKQGGYLIEGGEDSTGTLMSVINEIMALFK"
     gene            complement(3777..3849)
                     /locus_tag="AC16V7_00610"
     tRNA            complement(3777..3849)
                     /locus_tag="AC16V7_00610"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3813..3815),aa:Glu,seq:ctc)
BASE COUNT          952 a         1043 c          937 g         1040 t
ORIGIN      
        1 tggcctgtgt ttagctgatt tgatggcata gctgtgaaga ttattcgccg gttttgccgt
       61 caatgctgtt taccaatcca tcttttacca attttggaac ggtcacggta attgttcaat
      121 agctaacgcc atgtcaaatc agcctgtgat attttgcgcg gtcttagctt ggacctgttc
      181 tttgaaactc gcgattcttt tcagccagga cactgaatct accgatgtta agaaattcca
      241 agaaaatgga tcaccttcac ggtcaaatcc actataccag tgttgcctcc accgttgtct
      301 ttaatcaatt aagggccccg aagggccctt gttattgact gaggtgagcc gaagcccacc
      361 tcacattctt caattacttg aagagtgcca tgatctcatt gattacgctc atgagagtgc
      421 cggtggagtc ctctccgcct tcgatcagat aaccaccctg cttggtgtgc agattaaagg
      481 ccgtaagctc aacagcttcg tcttctttaa tcattaccgc acggtgacat ccaccacagg
      541 ctctcgaagc tgggccggtg acatattctg gcacttcacc gatttttcta tcccagccat
      601 tgactggatc ggatgccgaa agcagacctg gcagcgattt ggattgatcc ggcacgttgt
      661 tcgtgccatc cacgtggcag gagctgcagt ctgttattcc atgcttagga tacgggaact
      721 cgatgtgatg ctcatagtgc atggcttcca ccggatcagc aaagtcgata tcaccaatat
      781 cgaatgcttg gaatgagtgg atcgcatgga cataggaatc gatcgacctc gattgcattt
      841 ccagatgaga accgccactc ttggtgatgt gacacatcct gcagacaact acgtttccac
      901 ctctatccgg tgaatggaag gttgttgcaa gagcttcatg gcagttgtta cagccatcca
      961 caaccttaac gagagggccg tagaagtcat caacaaatgc attggcgcca aggtcgaagg
     1021 ttcgggaagg agcattcagc gcgagcacaa caccatcagc gttctccaag gtcggcatga
     1081 caccaatttc cacccgcatt actgtgccat cggcgatcat gtcagcccac gccgacaggt
     1141 ctgcagtgac ttcccacttg ccactgcctg cagagactgt cgtaaagcgc ggattttctt
     1201 cgcctacgcc atattccagg gctctcccat cgctcctgtc gatggtgcca tcgccattat
     1261 cgtcgaaaag cctctcgtgc gggccgatga tgaagtcctt ggtgtcccag ccatacaaac
     1321 cgaccatcac ggtgggggca atgtccgcca catcgagccc ctcaagatcc tgggtctcaa
     1381 ccgcgccgaa ttggatattg agcatgctgc cgtcaaagga tgcaccatca acggtgacgg
     1441 aaatggcttc agaatacttc agcccatcgg cggtgtagat cacatcgtta taaccggtgt
     1501 ggatctgatt gaacggtggt gctgccttgc cttcaccatg gcaactaagg cagtcagccg
     1561 tatccaaatc caacgaccca tgaattgctg ggggcaatat ggtcttcagt gcgagcgtgg
     1621 tggtatcgta agatggctct tcttgcccct ctttcacagg tcctactgta cctgtcacag
     1681 ggtggcagct cttgcaggtc gtgatattaa agttcgcatc cgacaacacc aaatccagtt
     1741 tgccttcgtg gcaggttacg cagtttgaca tggactgagg ataggggaat tccatggcat
     1801 gcgacatatg cacgtcattc atcagactgg tattatatgc gtactgggct ttctgctcat
     1861 ccgtcaattc aacatcgcct gcgaaatacc ctgcagccag ctctggatta tctaccaaaa
     1921 gctgccattc taaatggccg ccttcaccgt tatccaggtg gcaggcttta caggtgtaga
     1981 agtcagtagc gggatcctga ttcaactggg cataaatgta cccatgcttg aggtaaggat
     2041 ccgtgtggca tttttcgcag ccggcgttat tggcagccga aacgtagtct acgccgctac
     2101 cggtctctaa tattgccgca aacgggtatt tggtttgagc gatgcgagca ggtagtctac
     2161 caacgtcctc atccctaccg tacaaaacga cgaggccagg cgtactgccc acattagggg
     2221 catccccaac cagagtactc gtgcacgcac cagcgctatc acaagttata tcccccttca
     2281 gggacagtct tccagccgcc ggttcaaact ggaagtttgt accggtatat ggcgcaaagt
     2341 aaatacctag attatctgcg tcattggcat tgaaaggagc cccattcttg gtcatcttga
     2401 aagttaccac gctcttatcg ggagcagaaa acctataggc cagatcggta acttgaatga
     2461 caccgtcctg atacaactcg tcataggacg cctgatgctt gtcgccagct cctttatggc
     2521 aaattgcaca agtctcaggt tggacaacta gttctggttc tgcgacagag acttcagcgg
     2581 gcgctccagg ttcgcccgct gatcctgccg gaccagccgg accctccgga ccttgcggac
     2641 caggggggcc tgcggggcct gcacaagctg atagcaccag tacaagcccg agaatcaaaa
     2701 tcccaaaaat caggtgtttt tgtttcattt gcattctcca ttctcatatt attaatttca
     2761 tacgtaaagg caggttctct ccattatgta ttcttgatgt ataaactacc tcctttcact
     2821 ttttgacaga aatccgacgt tgctgatgaa ttatggaagt gggagtaaag ccctttccct
     2881 atggatattt ttcagttaat tctgtagctg tccggcaatc cgaagcggac cttgtggttg
     2941 agcgctacac acctgacccg cgcttcggtt tgatgacgga tcacctcccg agcgttaaga
     3001 cggtcgctaa attgaatata aaccgaaaaa tggctgtttc ggtaaatgaa cggcgatgat
     3061 agctgttttg ttcgttccat tcccctcccg tattcacaaa tccggcgtaa gatttcacgg
     3121 cgcggatggt tccagatcca ttatctcacc agaaaacgac atcgcagcag gattgaatct
     3181 tgatcttgcg cacccctttg gcggcggatg tcttgtgtat gcattctcta agtcataagc
     3241 gccatcaaca aaaattgttc aatttcggtc tgcgtttgtt gctgtaacca gctactgttt
     3301 cagtagtatc cacacccagg tgcattgttt gccataacaa tgctgtcgca ttttctactc
     3361 accctcccca aataacttat aattcggtgc tcccaatccg gaaaaatcga tcttttagaa
     3421 atcttgtctg ccccttttat taatgcttga ttagttagaa ttttcacatt ctatcatgat
     3481 tctccgtaat tgggtgatca aatttgtcag gtgaataaca gctgattgtc agaatccaag
     3541 accatgtaat caacctgagc gacttcaagt ggcaggtcgg caacaactag acaggctctg
     3601 cggcttgagc gtgggcagtg aaggcgttct gcaactttaa ttcgggagcc aagccggtag
     3661 tgaccagttt cccttttcgt tcatcatctg gtcagcaccc gggctgtggt cgaatgccac
     3721 cactccttta taaatataga caaaaaccgt tcaagatcaa cttgaacggt ttcggatgcc
     3781 cccaagggga ctcgaacccc tgttttggcc ttgagagggc cacgtcctgg gccactagac
     3841 gatgggggct gagagcgcag aaattctacc atgccaattt accaacgtca acatcccacc
     3901 tcttcctcca gggcaattgc atatgccctg aggggcatgc gcactcccgc aaagagagtg
     3961 tttggggtgg ga
//
LOCUS       DBNHOH010000040         8158 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_898988, whole genome shotgun sequence.
ACCESSION   DBNHOH010000040 DBNHOH010000000
VERSION     DBNHOH010000040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8158)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8158)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8158
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_898988"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            539..1156
                     /locus_tag="AC16V7_00615"
     CDS             539..1156
                     /locus_tag="AC16V7_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255647.1"
                     /translation="MSKQINDFIRQTYQYFYIDGLPELASGSMYLVTGILLAVMTRVE
                     SGTLAAGLVGLALIPIILGGIYLQNRLLRHLKERITYLRSGYVSYRRERKDSRRWIVI
                     VVALLLPFVLTLLPEQWQDLPLVIGGILGSVHIYMGYRLSIKRFYIEGAFAVFLGIVA
                     AIFLNNEVLGAAIIFLGAGIVLMAAGGLTLRHYLQTHPLPDEVTG"
     gene            1153..1455
                     /locus_tag="AC16V7_00620"
     CDS             1153..1455
                     /locus_tag="AC16V7_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR6255648.1"
                     /translation="MNEQLRDLVDIDRTIHEPARLAILAILWAAESVDFLYLLNAIEL
                     SRGNLSSHLSKLEAAGYILIQKTFEGKIPRTVCRLTDTGKAAFEGYKEQMRAFLAG"
     gene            complement(1612..2127)
                     /locus_tag="AC16V7_00625"
     CDS             complement(1612..2127)
                     /locus_tag="AC16V7_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255649.1"
                     /translation="MLNKAYLFYILVIALVTAGCQPGESGDFAIYLLAQDVASVELAK
                     VDLDQLALEEGPIISAEDIVTYDQTTHEIELTQAAYARIQQIFPLPVKVDGIPFVVCV
                     GEQPIYAGAFWTPLSSLSFDGVVIMQPFDTDGTTIRIVLGYPGPDAFMGRDPRADERV
                     MKALQAAGKLK"
     gene            complement(2510..3346)
                     /locus_tag="AC16V7_00630"
     CDS             complement(2510..3346)
                     /locus_tag="AC16V7_00630"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HIR6255650.1"
                     /translation="MSTLLAILAHPDDESFGMGGTLALYAQRGMAVHLVCATRGEAGD
                     VDERYLRGFDSKAERREAELRCAAEKLGLASVHFLDYRDSGMPGMPDNQHPQALIAAP
                     QADVVAKVVHLIRELRPQVVLTFDPIGGYKHPDHITIHNATVQAFELAGQDRYVDGLP
                     AHQPDKLYFHVIPKGYLRFAVRVMPLLGKDPRRLGRNGDINLVSLVEDGDFPIHAVID
                     YRAVAELKDDASLCHASQLDGASLRRGPMRWAQVLFGQKDHFMQAYPLPENGRRKHDL
                     FV"
     gene            complement(3445..4599)
                     /gene="moeB"
                     /locus_tag="AC16V7_00635"
     CDS             complement(3445..4599)
                     /gene="moeB"
                     /locus_tag="AC16V7_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HIR6255651.1"
                     /translation="MLPFLSHGEILRYSRHLLIPEVGLEGQKKLKAASVLVIGTGGLG
                     SPVSLYLAAAGVGRIGLVDYDVVDFSNLQRQVIHGTSRIGTLKVESARDRMLDLNPDI
                     QVDIYNEPFTSENAMRIAADYDLIIDGTDNFPTRYLTNDVCVLLGKPNVYGSIFRFDG
                     QVSVFYAKEGPCYRCLFPEPPPPGLVPSCAEGGVLGVLPGTIGSLQATEALKLILGIG
                     DTLIGKLMLYNALDMTFEYVKLRKNPNCKICGPNPEVTELIDYEAFCGVPGHDHEEGS
                     VGGGWDITATELSQRLQQGNHIHLLDVREPHELQISKLEGADLIPLGHLAARLSELDS
                     AQEMVVFCKAGTRSARALELLVSAGFRKVKNLKGGINSWARDVDPSMPIY"
     gene            complement(4652..4909)
                     /locus_tag="AC16V7_00640"
     CDS             complement(4652..4909)
                     /locus_tag="AC16V7_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HIR6255652.1"
                     /translation="MPTPLRTYTNGQAEIKLGGETVAAAMNDLVEQFPALRPHLFNGK
                     SELRPFVNLFLNDENIKALQGLDTPLKEGDRLMLIPSIAGG"
     gene            complement(5009..5713)
                     /gene="cysE"
                     /locus_tag="AC16V7_00645"
     CDS             complement(5009..5713)
                     /gene="cysE"
                     /locus_tag="AC16V7_00645"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - L-serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - L-cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HIR6255653.1"
                     /translation="MWKTIRRDIAAVFERDPAARSTLEILFCYPGLHALWGHRLAHWL
                     WTRRLKLLARWVSQLARGLTGIEIHPGASIGPGFFIDHGMGVVIGETSEIGADVTMYH
                     GVTLGGTSLSKGKRHPTIGNRVVIGAGAKVLGAITVGDDSRIGANAVVVKSVPVNSVV
                     VGVPGQIVSRSRPHLATDAPDLNHTALPDLIGVSLSSLLVRVDALEARLNGHASELHH
                     IHAPESGQWRGEDFSI"
     gene            complement(5717..6649)
                     /gene="cysK"
                     /locus_tag="AC16V7_00650"
     CDS             complement(5717..6649)
                     /gene="cysK"
                     /locus_tag="AC16V7_00650"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019344 - L-cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HIR6255654.1"
                     /translation="MKIANDVTELIGNTPLVRIRRLSDGAKAEIVAKLEFYNPAHSVK
                     DRIGVSMIDAAERAGLIGPETIILEPTSGNTGIALAFVCAARGYKCVLTMPDTMSMER
                     RLLLRAYGAELVLTPGSEGMAGAVRKAEEMAASDSRYLIPQQFKNPANPEIHRRTTAE
                     EIWRDTDGKVDILVAGIGTGGTITGVGEVLKKRKPSFRCVAIEPAASPVLSGGQKGPH
                     MIQGIGAGFVPDVLNTQIYDEVVRVTNEDALETARRMAREEGLLVGISSGAAMWAAMQ
                     VARRPENEGKLIVVIIPSFGERYLSTALFAELAG"
     gene            complement(6671..6961)
                     /locus_tag="AC16V7_00655"
     CDS             complement(6671..6961)
                     /locus_tag="AC16V7_00655"
                     /inference="COORDINATES: protein motif:HMM:NF023174.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HIR6255655.1"
                     /translation="MSLYFVRHQHSPETCPAKDPQMGSMLLTHINPMNARKFGVNLIG
                     DAVLDGQHTFVVIAEADSPANIENFMQPFKMAGPVEISPASTCEVVVERLGC"
     gene            complement(6964..7395)
                     /locus_tag="AC16V7_00660"
     CDS             complement(6964..7395)
                     /locus_tag="AC16V7_00660"
                     /inference="COORDINATES: protein motif:HMM:NF025819.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008235 - metalloexopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HIR6255656.1"
                     /translation="MLEIASEVLKKIHAHGEKAYPEEGAGFLLGKVDGEQRRVTAILE
                     LVNAREDAARHNRYLLTPQDYLRGEQEADRLNLDVLGVFHSHPDHPSLPSEFDRQWAM
                     PWFSYLITSVQAGRAAGSRVWRLVEDRSQFDEEKIEITNRS"
     gene            complement(7452..>8158)
                     /locus_tag="AC16V7_00665"
     CDS             complement(7452..>8158)
                     /locus_tag="AC16V7_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HIR6255657.1"
                     /translation="DSTSGNMGIAYATFGAALGVPVTLALPANASPERQTVLRALGAE
                     LILTDPLEGSDGAIRFARQLAMDNPEAYYYANQYDNPANWQAHYHATGPEIFQQTGGQ
                     VTHFIAGLGTSGTLTGVGRYLRRSGTQVQLIAVQPDAAFHGLEGLKHLPTAIQPGIYD
                     SSMPDRILEISTEAAYAMVRRLAREEGLFVGISSGAAAVAALQVAAELDEGVVVTVFP
                     DAGYKYLSDDALWDEA"
BASE COUNT         1934 a         2306 c         2187 g         1731 t
ORIGIN      
        1 gtgtcaaaga tcaatgcgcg atcgcccaga tcgatgatgc cagcattgct gtatgccgca
       61 ccaccaataa ggccgatcgc ggcgtagacc ccctcggcta tttgatagag ctcaaaatgt
      121 tcagaaacct ggagtttacg aaaaatctgg ttcatattta actcacctct cgaaccacag
      181 agaaatttgg atcgtctaaa aacctcatac aggatgccgc tcagaatggg tgatgcagac
      241 caatactgga tgagataaac ccattgtgcg acgggaacga actccaaatc gacacaaata
      301 ctttaccagg taaagcgctc tatctgtata ccattgattg cgggaagagg gcaagggaat
      361 tgcacaaaca gagattcggc aagcacttcg gctgacacag atagagaaac acagctttcc
      421 tgtgaaaaca gccagataga gtaagaaaca gcccttgaca attctacaac cacctgctaa
      481 aatgatacag atgagtttgc aatacaaact agttcgtgtt acataaccgg agtcaatcat
      541 gagcaagcaa atcaacgatt ttatccgcca gacctaccag tatttttaca tcgatgggct
      601 gccagaacta gccagcggca gcatgtacct ggttactggt atcttactgg cagtcatgac
      661 ccgcgtcgag tctggaacgc tcgccgcagg tctggttggg ctggcactta tccccatcat
      721 cctggggggg atttacctgc aaaaccgtct tctacgacac ctcaaggagc gaattactta
      781 tctgcgatct ggctatgtct cctaccgacg cgagcgcaaa gatagcaggc gttggatcgt
      841 catcgttgtc gctttgctgc ttccgtttgt tctgaccttg ctgcctgagc aatggcaaga
      901 ccttcctctg gtcattggag gaatcctggg ctcggtgcat atttatatgg gataccggct
      961 tagcatcaag cgcttctaca tagaaggtgc gtttgccgtt tttttgggaa ttgtcgcagc
     1021 catatttctg aacaatgaag tgcttggtgc agcgatcatc tttctcggtg ctggcatcgt
     1081 gctgatggca gccggcgggc tgacgctgcg acactatcta caaactcatc ctttgcctga
     1141 cgaggtgaca gggtgaacga gcagctgcgc gatctggtcg atatcgaccg caccatacac
     1201 gagccagccc ggctggctat cctggcgata ttatgggctg cggagagcgt agatttctta
     1261 tacctgttga acgccatcga gctcagccgg ggaaacctgt cttcccatct cagcaagcta
     1321 gaagcagctg gatatatcct gatccaaaaa acctttgaag gaaaaatacc caggacagtc
     1381 tgtcgcttga cggatacagg caaagcggct ttcgaaggct ataaagagca gatgcgcgcc
     1441 ttcctggcgg gataacgacc agattgtcca tcgctctggc agctatagac gggggatctc
     1501 gaggccatgc gtcaaaccgc agttggggag attacggtga tcccagtgcc ccccattccg
     1561 agatccagga acgagctgga atagaaaagg gcctcacaca acagccccgt tctactttaa
     1621 cttacctgct gcctgcaagg ctttcatcac ccgctcatcg gcgcgcgggt ctcttcccat
     1681 aaatgcatcc ggcccgggat aacccaatac gattcgaatc gtggtcccgt cagtgtcaaa
     1741 tggttgcatg atgacaaccc cgtcaaaact gagggatgat agcggcgtcc agaatgcacc
     1801 ggcatagata ggttgctccc ccacacaaac cacaaaggga atgccgtcca ccttaaccgg
     1861 caaagggaat atctgctgaa tgcgagcata ggcggcctga gtgagctcta tttcgtgggt
     1921 ggtttgatcg taggtgacaa tgtcctccgc cgagataatt ggcccctctt caagggctaa
     1981 ttgatcaaga tcaaccttgg caagctccac agaggccaca tcctgggcaa gcaaatagat
     2041 ggcgaaatcg ccgctttctc ctggctggca cccagcggtt accaaagcga tgacgaggat
     2101 ataaaagaga tatgccttat tcaacacgaa tgcctcctga ggaccacatt ttacaatgat
     2161 cttacccccg ctgtggtagc actgcactta accgtggcgc ttcccctgca ccccaggggg
     2221 tgaggagaag aggatgcttg tcttataggg gattaatgtc accgatccaa gcggacccac
     2281 cagctgggca aagcggttaa atggctagtg ttggtgatgg tggtgttcat cctcacggga
     2341 tgagggatct gcttttcaac agcctggctc accatcatgc ccacaacagt tctggcgcaa
     2401 caacaccctg cgccagaggt tgcgagtaag ctttgccatc ttctctggaa agggcatggg
     2461 ttggtccaaa ttggtcaaga aatcggaaat cgaaggtcgt ctcatcattt cacacaaata
     2521 ggtcgtgctt acggcgaccg ttctcgggca gcggataagc ctgcatgaag tgatctttct
     2581 gcccaaagag cacctgcgcc cagcgcatcg gcccgcggcg cagcgaggcg ccgtcgagct
     2641 ggctggcatg gcacaatgag gcatcatcct ttaactcagc cacggcgcga tagtcaatca
     2701 cagcatggat gggaaaatcc ccatcctcca ccagggaaac caggttaata tcgccattac
     2761 gcccgagacg gcgcgggtct ttgcccagca agggcatcac acgcacggca aagcgcaggt
     2821 agcctttggg gatgacatga aaatagagtt tgtctggctg gtgggcaggc agcccatcca
     2881 catagcggtc ttgcccggca agctcaaagg cctgcacggt agcattgtgg atagtgatat
     2941 gatccgggtg cttgtagccg ccaataggat caaaggttaa gacaacctga gggcgtagct
     3001 cacggattaa atgtaccact ttcgccacca cgtctgcttg cggggcagcg atgagggcct
     3061 gagggtgctg gttatcaggc atgccgggca tgcccgaatc gcggtaatcc aggaaatgca
     3121 cgctcgccag ccccagcttc tcggcagcgc agcgcagctc ggcttcgcgg cgctcggctt
     3181 tggagtcaaa cccccgaaga tagcgttcat ccacatcacc agcttcacca cgcgtcgcac
     3241 aaaccaggtg cactgccata ccccgctggg cataaagggc caatgtgcca cccatcccaa
     3301 acgactcgtc gtcagggtgg gcaagaatag caagaagagt gctcatgttc attgatgcaa
     3361 atttcaagat tgtcgattgc agattgccga ttgttgatta atgagcgaat atatcttcaa
     3421 tcaccaatct ttaatccaaa atcactaata gatcggcatg cttggatcga catcgcgcgc
     3481 ccaagagtta atcccaccct tcaggttttt caccttgcgg aaaccagcgc tgaccagtaa
     3541 ctccagggca cgagctgaac gggtgccggc tttgcagaaa acgaccatct cctgggcgct
     3601 gtccagctca gacaggcgcg ccgccaggtg tcccagtggg atgaggtcgg cgccttccag
     3661 tttcgagatc tgcagctcgt gcggctcgcg cacatccagc aaatggatgt ggttgccctg
     3721 ctgcaggcgc tgggacagct cggtggccgt gatgtcccag cccccgccca ccgaaccctc
     3781 ttcatggtca tgcccgggca cgccgcagaa ggcttcgtaa tcaatcagct ccgtgacttc
     3841 cggattgggt ccacagatct tgcaattggg attcttgcgc agcttgacat actcaaaagt
     3901 catatccaag gcattgtata acatcaactt gccgatgagc gtgtctccaa tacccaagat
     3961 gagcttgagc gcctctgtag cctgcagcga tccgatggtg cctgggagca cacccagcac
     4021 gccaccctca gcgcaggaag gaaccaatcc tggaggcggg ggttcaggga agagacagcg
     4081 gtaacaaggg ccctctttgg cgtaaaaaac actcacctgg ccgtcgaagc ggaagatgga
     4141 gccatacaca ttgggtttgc ccaacagcac gcacacgtca ttggtcaggt agcgggtggg
     4201 gaaattatct gtgccatcga tgattagatc gtaatcagca gcgatgcgca tggcattttc
     4261 cgaagtgaac ggctcattat agatgtctac ctggatatcg ggattgagat ccagcatgcg
     4321 atcgcgggct gattcgactt ttagtgtgcc aatgcgagag gtgccgtgga tgacctggcg
     4381 ttgcaggttg gagaaatcca ccacgtcata atccaccaga ccgatacgcc ccacgccagc
     4441 cgcggccagg tacaacgaga ccggcgatcc cagcccgcct gtgccgatga ccagaaccga
     4501 ggcggctttg agtttcttct gcccttccag ccccacctcg gggataagca agtggcgcga
     4561 gtagcgtaaa atttctccgt gagatagaaa aggtaacata gtcatttgag aaatgagatt
     4621 taagagtcga gagcgacact catctcgaaa ctcaacctcc cgcaatagat ggaatgagca
     4681 tcaaccgatc gccctctttg aggggcgtgt ctagcccttg aagagccttg atgttctcat
     4741 cgttgagaaa caggttgacg aacgggcgca gctcagactt accattaaaa aggtgcgggc
     4801 gaagcgccgg aaactgctcg accaagtcgt tcatggcggc agccaccgtc tcgcctccta
     4861 acttaatctc agcctgtccg ttggtataag ttcgtaaagg tgtaggcatt cgtagcgtaa
     4921 tcatactttg gtatttattc cttctgtaaa attgctccaa ctccctgccc tccttctctt
     4981 taagaaaagg agagagcttg ctggatgttt atatcgaaaa gtcctcgccc cgccactgcc
     5041 cggattcggg agcgtgtatg tggtgcagtt cggatgcatg cccattcaga cgcgcctcca
     5101 gggcatccac gcgcaccagg agcgaggata atgatacacc aattaggtct ggaagtgcag
     5161 tgtgattgag atccggggca tccgtagcca ggtgcggtcg gctgcgcgat acgatctgtc
     5221 cgggcacgcc caccaccacc gagttgaccg gcaccgactt gaccaccacg gcgttggccc
     5281 caatgcggct gtcgtcccca acggtgatgg cgcccaacac cttggctccc gcgccgatca
     5341 ccacccgatt gccgatcgtg ggatggcgtt tgcccttgga gaggctggta cctcccaagg
     5401 tgaccccgtg atacatcgtc acatcggcgc cgatctcaga agtctcgccg atgaccacac
     5461 ccatgccatg atcgatgaaa aagcccgggc cgatggatgc gccagggtgt atctcgatgc
     5521 ctgtcaggcc gcgcgccagc tgcgaaaccc agcgcgccag cagcttcagg cggcgcgtcc
     5581 acaaccaatg cgccaggcga tgcccccata aagcatgcag cccaggatag caaaacaaga
     5641 tttccagggt actacgcgcc gctggatcgc gctcaaatac ggcagcgata tctcgccgga
     5701 ttgtcttcca catggttcac cccgccagtt cagcaaatag agcggtgctc agatagcgct
     5761 cgccgaagga agggatgatc acaacgatca atttaccctc gttctccggg cggcgcgcca
     5821 cttgcatggc cgcccacatg gccgcccccg aggagatgcc caccagcagg ccctcttcgc
     5881 gagccatacg ccgggcggtc tccagggcgt cttcgtttgt tacgcgcacg acctcgtcat
     5941 agatctgcgt gttgagcaca tcgggcacaa agccagcgcc gatgccctgg atcatgtgtg
     6001 gacctttctg cccgccagag agcaccggcg aggcagccgg ttctattgcc acacatcgga
     6061 aggaaggctt gcgcttcttg agcacctcac cgactccggt aatagttcca cctgtgccaa
     6121 tgccggcaac cagaatatca accttgccat ctgtatctcg ccaaatctct tcggcggtcg
     6181 tccggcggtg gatttcaggg ttggccggat tcttgaattg ctgagggatg agatagcgcg
     6241 aatctgaggc ggccatctct tccgctttac gaaccgcgcc agccatcccc tcgctacctg
     6301 gcgtgagcac cagttcagcg ccgtaggctc gcaggagaag gcggcgttcc atgctcatcg
     6361 tatcgggcat ggtaagcacg cacttatagc cccgcgctgc gcagacgaaa gccagagcaa
     6421 tacctgtgtt accgctggta ggctccagaa tgatcgtctc gggaccgatc agccctgccc
     6481 gctctgcggc atcaatcatt gacactccaa tgcgatcctt aacgctgtgc gccgggttgt
     6541 aaaactctaa cttggcgaca atctccgctt tggcgccgtc gctcaagcgg cgaatgcgca
     6601 ccaggggagt attaccgatc aattcagtga catcattagc aattttcatc tctgtctccc
     6661 ccgctcaaga ctagcaaccc aggcgctcaa ccaccacttc acaagtggaa gcagggctga
     6721 tttcaacagg accagccatc ttgaacggct gcatgaaatt ctcaatatta gccgggctgt
     6781 cagcctcagc gatgaccacg aaagtgtgct gaccgtccaa cacggcatcg ccgatcaagt
     6841 tgacgccgaa cttgcgggca ttcatcggat tgatatgtgt aagcaacatc gagcccatct
     6901 gcggatcttt agccgggcaa gtttcagggc tatgctggtg acgtacaaag taaagggaca
     6961 tggttaactc ctatttgtaa tctctatttt ctcttcatcg aactgcgacc gatcttctac
     7021 caagcgccag acgcggctcc cggccgcgcg accggcctgc acgctggtaa tcaggtagga
     7081 aaaccagggc atagcccatt ggcggtcaaa ctcggaaggc aggctgggat ggtcgggatg
     7141 ggaatggaac acgcccagca catccagatt caatcgatca gcttcctgtt cgccacgcag
     7201 gtaatcctga ggggtaagca ggtagcggtt gtgccgggca gcgtcctcgc gggcattgac
     7261 caactccagg atggcagtca cccgacgctg ctcaccatcc actttgccca acaagaagcc
     7321 cgctccttct tcgggatagg ctttctcacc gtgagcgtga atcttcttta aaacttcaga
     7381 tgcaatttcc aacatgttta ttaccaaatc ttgtagctca agcgaaatgc gagcaacgat
     7441 taactctcgt tttaagcttc atcccacaag gcgtcatcgc tcagatattt atagccggca
     7501 tcaggaaaaa cggtaaccac cacgccctcg tccaattccg cagcgacctg cagggctgct
     7561 accgccgctg cgccggagga gatacccaca aaaagaccct cctcacgagc gagccgccgc
     7621 accatcgcat aagctgcctc ggtagatatc tccaggatgc ggtcaggcat ggaggagtca
     7681 tatatcccag gttggatggc ggtaggcagg tgcttaagac cctccagccc atggaaagcg
     7741 gcgtcgggtt ggacagcgat cagttggact tgtgtgccac ttcggcgtag atagcgccct
     7801 acgccggtga gcgtgccaga cgtgcctagc ccggcgataa agtgggtgac ctgcccgcct
     7861 gtctgctgga aaatttcggg gccggtagcg tggtaatgag cctgccaatt ggcagggttg
     7921 tcatattgat tggcgtaata ataggcctca ggattatcca tggcgagctg ccgagcaaag
     7981 cggatggcgc catcagagcc ttccaagggg tcagtcagga tcagttccgc tcccagggca
     8041 cgcaggacag tctgccgttc tgggctggca ttggcaggca gcgccagggt gaccggtaca
     8101 ccgagcgcgg ctccaaaagt agcgtaagcg atgcccatat ttccagaagt ggaatcga
//
LOCUS       DBNHOH010000041         1143 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_66183, whole genome shotgun sequence.
ACCESSION   DBNHOH010000041 DBNHOH010000000
VERSION     DBNHOH010000041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_66183"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(265..939)
                     /locus_tag="AC16V7_00670"
     CDS             complement(265..939)
                     /locus_tag="AC16V7_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255658.1"
                     /translation="MDLILHLSSRFSHRKFLFIGSLCCKRDKKIQDFEHHIQLIRSNV
                     TLINVSGYGINEIHNLVKYWIGSFNGSQAFSEKLLQICGGNPYYLHEIVKWIAESGLS
                     AKELDENPNIPIPNTISKTIEARLGRLEPVEREVLEYASLLGRKFRFEELHSCSDLPL
                     THILDTLDNLVNRHLLVVHSTNYQFRHELIRFFILNSMNPARRQAMEQNFAHEPDVNL
                     VATILE"
BASE COUNT          284 a          261 c          278 g          320 t
ORIGIN      
        1 atcgttgtgt ccacagccaa ccggatcgag gaataggcta cctcgggaac gtgaaccgtg
       61 attttctccc cggcaatgcc cttaccgctt ggttggctgt tggtcattac cactatagca
      121 agaacaggtc aacgctcttg ctacattaat gctacaagag tggccgttgt ggaattcttg
      181 tattcgaagg cgagtattta tctttgtcgt gccaatgcaa ttgagcggtt cgctttgctt
      241 gattccccct ggatcagcga gacctcattc caaaattgtc gcaaccagat tcacatccgg
      301 ttcgtgtgca aaattttgct ccatggcctg gcggcgcgct gggttcatgc tattcaaaat
      361 gaagaagcgg atcaattcgt gacggaactg gtagtttgta gagtggacaa ccagcaggtg
      421 ccggttaact aaattgtcca gggtatcgag gatgtgtgtc aggggtaggt cgctacaaga
      481 atgtaattcc tcgaatcgaa acttgcgacc cagcagggag gcatattcca gaacttcccg
      541 ttcaaccggc tccaggcgac caagccgcgc ttcgatcgtt ttggaaatgg tgttggggat
      601 cgggatgttg ggattttcat ctaattcctt tgcagacaac ccggattcag cgatccattt
      661 tacgatctcg tgcaggtagt aaggattgcc tccacaaatt tgcaggagct tctctgagaa
      721 ggcttggctt ccgttaaagc tccctatcca atatttcacc aggttatgga tctcattgat
      781 gccatatcca gatacattga tcaaggttac gttggagcgg attaactgta tatggtgctc
      841 aaaatcctgg atttttttat cacgcttgca gcaaagcgaa cctataaaca ggaacttccg
      901 gtggctgaac ctgctggata gatgcaggat taaatccaac gtattctcat ctgcccactc
      961 taaatcttcg aggcagagaa tgacgccatt agaagcgctc aatagcttct cgaaaaacct
     1021 tgaaatcagc ttgaaaaaat actccttctg gtcgatttta gtgatctcac tggcctcagt
     1081 ccatttagat gtggtaagtg aaatggattc tgctatctgg gctatctcat gtgttgaatc
     1141 ggg
//
LOCUS       DBNHOH010000042         5567 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_194207, whole genome shotgun sequence.
ACCESSION   DBNHOH010000042 DBNHOH010000000
VERSION     DBNHOH010000042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5567)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5567
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_194207"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..637)
                     /locus_tag="AC16V7_00675"
     CDS             complement(158..637)
                     /locus_tag="AC16V7_00675"
                     /EC_number="7.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="QcrA and Rieske domain-containing protein"
                     /protein_id="HIR6255659.1"
                     /translation="MEKEEQITRNNFISMVIWAVGGLISLGMGIPAIAYIIGPALSRN
                     QVQEWIRLGPISKIELNIPTLFKFVIQRKTGWIVNEEEISVYVLTDNGRDFIAMSNIC
                     SHLGCRVRWISDQEIFFCPCHNGVFDKEGAVEAGPPPGPLDRYELKVEDDQLFILGG"
     gene            complement(660..2285)
                     /locus_tag="AC16V7_00680"
     CDS             complement(660..2285)
                     /locus_tag="AC16V7_00680"
                     /inference="COORDINATES: protein motif:HMM:NF015237.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HIR6255660.1"
                     /translation="MPYALLGVLTLMLLTAAAYAWDYTNSPGFCGQACHTMPPEYTAY
                     LLSPHARVDCVDCHIGKGFIATRISRKASDLRHVYATAFVSYEFPIRADDLRPARETC
                     ELCHSPQKFSDDSLREIKRFGDDRANTPISIYLVLKTGGGTRREGLGRGIHWHIENKI
                     DFLATDGHEQEIPYVRVIEDDGSITEYMDLETNIVPSSIKDSDLKRMDCISCHNRITH
                     MVLSPVDTVDKLMARGLISSDIPEIHQKAVNVYSTVFQNTEVGLNRIASLREFYKTYY
                     PDYYAENQEKVEAAISALQQAYSNSVYPEQSSDWTTHPSNVGHQNSPGCFRCHDGKHL
                     NQQGEAIRLECNLCHSIPVVAGPNEFVADIEISRGPEPETHLNPNWISQHRDVFDPTC
                     SNCHTTDNPGGTDNTSFCSNSACHGSAWEYAGFDAPGLREIILAQLPPAPTPVPLPSG
                     ESTTYEATIGPLFEMRCGSCHGATSSIQGLNLGIYEDAMKGGDSGSVIVPGDPQGSLL
                     IQKQSGEQAHFGQFTSEELDLIVQWIQDGAPEN"
     gene            complement(2454..3701)
                     /locus_tag="AC16V7_00685"
     CDS             complement(2454..3701)
                     /locus_tag="AC16V7_00685"
                     /inference="COORDINATES: protein motif:HMM:NF024827.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HIR6255661.1"
                     /translation="MKHRWILNLGVLLLALVLLAACASPQGPAGDSGPPGPPGPEGPQ
                     GPAGEQGPAGPEGKVGEAAAEYIGAATCGGCHQELYDAFMKSGHAWKLNPVVDGQPPE
                     YPFTEVDQLPEGYTWDDISYVIGGYNWKARFIDQNGYIITDKPGAVISDTTYLNQWNF
                     ANPMVGKEAGWVTYNSGAKELEYTCGNCHTTGYSPGGNQDGKPGLVGTWEEPGIKCEE
                     CHGPGSLHASNPRGFAMQIDRDAELCGQCHIRNAIEAVDAEDWFINHHEQYEELFQSK
                     HVTIDCVVCHDPHKLVVQLRQSGQATTRTQCQNCHFKQAQFQNNSKHVAMNIACIECH
                     MPRIVKSAWGDPATFTGDIRTHLMAIDPNQIDQFSKDGTTALSQISLDFACRHCHVEG
                     RGFPKTDEELKAVANGYHNLPEP"
     gene            3986..5521
                     /locus_tag="AC16V7_00690"
     CDS             3986..5521
                     /locus_tag="AC16V7_00690"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6255662.1"
                     /translation="MNAPELQFDIVVVFFIYGLAFFSMGLTVMMEARRSPMLAEARVL
                     WPLAFFGFVHGSHEWLEIGLDVRRWFGFNDPLYIPWIRLVLLVISFASLVLYSLQVLR
                     PQGRVLSQSNFQTVLALLGLYLILVIAIGLIQPNSLEVLQRRADALARYALAVPGAGL
                     AALALIRQASQVPTTERRELTIGLHLAALGFAIYSLTQIMVTPVDIFPARYLNSTAFL
                     NWAGFPIQAVRACMAVLVTVGLIQATQVVEGDRQRQLASAQEARLRALEQIRLELAER
                     EALRRELLRHTVAAQEEERSRIARELHDETAQFLTALSLDLATLRKSLHRNKEAVHIL
                     DRLQDMRSKMSQGIYRMVHDLRPAQLDDLGIVAALGYLVDDTRHSGLQVVLNVEGNRQ
                     RLDPLNETVLFRIAQEALTNVTRHSGADKATVVLAFEPCQIRLAIQDEGSGFTPNESS
                     HPPRGWGLVGMRERAESVGGSFHIHATPGIGTLIEVIVPLSDTQPAPIEENNHEPNPV
                     DAC"
BASE COUNT         1252 a         1485 c         1438 g         1392 t
ORIGIN      
        1 tatacccgtc acgatctggt tgacagccaa gaacagggaa gcgctgccaa gcgtaaaaag
       61 ccaatttact ttcggaatct tccgaaggaa tatggctgtc caaacttgac gccaagccag
      121 acgctcatcg agccaattcc caatttttgt agccaactta acctcctaaa atgaacaact
      181 gatcgtcttc tacctttaat tcgtaccgat caagtggacc gggcggagga cctgcttcca
      241 ccgctccttc tttgtcaaaa actccgttat gacaagggca gaagaagatc tcttgatcag
      301 agatccaacg cacccgacac ccaaggtgag aacagatatt ggacatggcg ataaaatcgc
      361 gcccgttgtc ggttaacacg taaaccgaaa tttcctcctc attcacaatc caaccagttt
      421 tgcgctggat cacgaattta aataaggtag ggatgtttag ctcgattttt gatatcggtc
      481 caagccgaat ccattcttgt acttggttgc gactgagggc tggaccgata atatacgcga
      541 tggcaggtat tcccatgcca aggctgatta atcctccaac agcccaaatc accatgctga
      601 taaagttatt gcgagtaatt tgctcctctt tttccatttc tccatccaat aaggttaatt
      661 tagttttccg gcgcgccgtc ttgaatccac tgaacgatca aatccagctc ttcggatgta
      721 aactggccaa agtgtgcttg ctcaccggat tgtttttgta ttagcaggct gccctgcggg
      781 tctcccggaa cgatgacaga gccactgtcg ccgcccttca ttgcgtcttc atatatgccc
      841 aagttcaggc cttggatact gctggtggcg ccatggcaac tgccacagcg catttcaaag
      901 agcggaccga ttgtggcctc ataagtggtt gactcaccag aaggcaaggg taccggtgtg
      961 ggggcagggg ggagttgtgc caggatgatt tcccgcaatc caggcgcatc gaaacctgca
     1021 tattcccagg cactgccgtg gcaggcactg ttcgagcaaa acgacgtgtt gtctgtgcca
     1081 cccggattgt ctgtcgtgtg gcagttagaa catgtggggt caaacacatc ccggtgctgg
     1141 gaaatccagt ttgggttcag gtgcgtttct ggctcgggtc cacggctgat ctcgatatcg
     1201 gccacaaact cattcgggcc tgccaccaca ggtatcgagt gacacaagtt gcactccaag
     1261 cggatagctt cgccttgctg gttgaggtgt ttgccatcat gacagcggaa gcaaccaggt
     1321 gagttctgat gacccacatt gcttgggtgg gtagtccagt ccgaactttg ctccggatat
     1381 acgctattgc tatatgcctg ctgcaaagcc gaaatagctg cctcgacctt ctcctgattc
     1441 tcagcgtaat agtccggata ataggtctta taaaactccc tcaggctcgc gatacggttc
     1501 aagcccacct cggtattttg gaaaaccgtg ctatagacat tcactgcctt ttgatggatc
     1561 tcggggatgt ccgacgagat taagccgcgt gccatcaact tgtctactgt atctacaggg
     1621 gataacacca tatgggtgat tcggttatgg caactgatgc agtccatacg cttgaggtcg
     1681 ctgtctttga tcgacgacgg gacaatattg gtctctagat ccatgtattc tgtgatcgaa
     1741 ccgtcatctt cgatcacgcg cacataggga atctcctgct catgaccatc tgtcgccaga
     1801 aaatcgatct tgttttcaat atgccagtgg atgccgcgcc ccaacccctc tcgccgggtg
     1861 ccgccgccgg tctttagcac caggtaaatg ctgatgggtg tgttggcgcg atcgtcgcca
     1921 aagcgtttga tctctcgcag gctgtcatct gagaatttct gtggagaatg gcacagctca
     1981 caggtctcgc gcgccgggcg caagtcatcg gcgcggatcg ggaactcata cgatacgaac
     2041 gccgtagcat acacgtgcct gagatcactg gcctttcgag aaatacgcgt ggcgataaat
     2101 ccctttccga tatggcaatc cacgcagtcg acacgggcat gcggcgaaag caaataggct
     2161 gtatactctg gcggcatggt atggcaagcc tggccacaga acccaggaga gttcgtataa
     2221 tcccaggcat aggcggcagc ggtgagcagc atcagtgtca gcacacccaa caaagcgtag
     2281 ggcataagcc gcacccatcg agatgcgtca acaggaggga aaaagaagcg tttaagactt
     2341 tgaattattt ttttcattcg tatatatacc atttgtgtga tggaaggtca tcacccatcc
     2401 atcacactga agaagatcat ctccttccaa aaggcaggaa aatttgtgaa tagttaaggc
     2461 tccggcagat tatgatagcc gtttgcaaca gccttcaact cttcatcggt cttggggaaa
     2521 cctctgcctt ccacatggca gtggcggcaa gcaaaatcca ggctgatttg cgagagagct
     2581 gttgtgccgt ctttgctgaa ttggtcaatc tgattcgggt caatcgccat aagatgggtg
     2641 cgaatgtcac cagtaaacgt ggcagggtca ccccaggcag atttgacaat gcgcggcata
     2701 tggcactcga tacaggctat gttcatggcg acgtgcttgc tattgttctg gaattgggcc
     2761 tgcttgaagt ggcaattctg gcactgggtg cgcgtggtcg cttggccgct ttgtcgcaat
     2821 tgcaccacta gcttgtgcgg gtcgtgacac actacgcagt cgatggtcac atgtttgctc
     2881 tggaatagct cttcgtactg ctcgtgatgg tttatgaacc agtcttccgc atccactgcc
     2941 tcgatggcat tgcgaatgtg gcactgcccg caaagctcgg catcgcgatc gatctgcatg
     3001 gcaaaaccgc gcgggttcga ggcgtgcaag ctgcccgggc catgacactc ctcgcattta
     3061 atgccaggtt cttcccatgt tcccaccaga ccaggcttgc catcctggtt gccaccaggg
     3121 ctgtaacccg tggtatggca gttgccacag gtatactcta actctttcgc gccggagtta
     3181 tatgtcaccc atccggcttc tttgcctacc atgggattgg cgaaattcca ctggttgagg
     3241 tacgtagtgt cgctaattac cgcgcctggc ttatcagtga tgatgtagcc attttgatcg
     3301 atgaagcggg ctttccagtt atagccaccg attacatagc tgatgtcatc ccaggtatat
     3361 ccttcgggga gctggtctac ttcggtaaac ggatattctg gcggctggcc atccaccacc
     3421 gggttgagct tccaggcatg cccagacttc ataaaggcat cgtatagctc ctggtggcat
     3481 cccccacagg ttgcggctcc aatgtattct gcggcagctt ctcctacttt gccttcaggg
     3541 ccggcgggac cttgctcgcc ggcaggtcct tgagggccct cagggccagg aggaccagga
     3601 ggaccggaat cgccagctgg accttgtgga ctggcgcagg cagccaacaa gaccaacgct
     3661 agcaacaaaa cgcccaaatt caatatccaa cgatgcttca taatagatgt attctcctca
     3721 ttgctgggca aaatgatgat ctagagttgg gaatcaccac acatttgcgg ttgaattttg
     3781 cccttccagg atatataatg tacttgggat tgggttatac acccaatata cgctcgtctt
     3841 gttgggtcac tcacaccaca cagtatacct taagtcaaga tgagagacta gtgaaatttt
     3901 tctcatagct tgggttatta gtcatatata ttagttgatt aacatcctct gatttgtgga
     3961 aaaacgggtg agctggcata accctatgaa tgcgccagag ttacaattcg atatcgttgt
     4021 cgtcttcttc atctatggat tggccttttt tagcatgggg ctaaccgtga tgatggaggc
     4081 caggcgttct ccaatgctgg ctgaggcgcg cgtgctgtgg ccgctggcgt tctttggatt
     4141 tgtgcacggt tcgcacgaat ggctggagat cggattggat gtgcgccgtt ggttcggttt
     4201 taacgaccca ctttatatcc cctggatccg gctggtgctc ttggtaatat cctttgcttc
     4261 gctggttctc tacagcctgc aggtgttgcg cccccagggc agggttttgt cgcagtcgaa
     4321 ttttcagacc gtgcttgcct tgcttgggct ttacctgatt ttggtcatcg ccattggatt
     4381 gatccagccc aattccttag aggtgttaca gaggcgcgcc gatgccttgg cacggtacgc
     4441 cctagctgta cctggggcag gcttagccgc tctggcgctt atccgccaag ccagtcaggt
     4501 gccaaccacc gaacgccgtg agttgaccat cggcttacac ctagccgccc tgggttttgc
     4561 catttacagc ctgacccaaa taatggtcac cccggtagat atcttccctg cccgatatct
     4621 gaactcgacc gcctttctga actgggcagg ctttcccatc caggcggtgc gcgcatgtat
     4681 ggcggttctg gtcaccgtcg gtctgatcca ggctacccag gtggtggaag gagatcgcca
     4741 aagacagtta gcctctgccc aggaagcacg ccttcgtgcc ttggaacaaa ttcgtcttga
     4801 gctggctgaa cgcgaagctt tgcgccgcga gctcttgcga cacactgtgg ctgctcaaga
     4861 agaagaacgc tcccgcatcg ctcgtgaatt gcacgatgag actgctcaat tcctgaccgc
     4921 tctcagcctg gatctagcaa cactccgcaa atccctgcac cgcaacaagg aagcagtgca
     4981 catcctggac aggctgcaag acatgagaag caagatgtcc cagggcattt accgcatggt
     5041 gcacgatctg cgccctgccc aattagatga cctgggcatt gtagccgcat tgggatactt
     5101 ggttgatgac acccgccata gcggactgca agtggttttg aatgtagaag gcaatcgcca
     5161 gcggctagac ccgctgaatg aaactgtgct atttcgcatt gcccaagaag ccctgaccaa
     5221 tgtcacccgc cattcaggtg ccgacaaagc cacggtggtc ctggcatttg aaccctgcca
     5281 gatcaggttg gcaatccagg acgagggttc tggctttacc cccaacgaaa gctcccaccc
     5341 gccacgtggc tggggtttgg taggtatgcg tgagcgtgct gagtccgtcg gtggctcgtt
     5401 tcatatccat gccacccctg gaattggcac cctgattgaa gtgattgttc cactttcaga
     5461 tacccagcca gcaccgatcg aggagaataa ccatgagccc aatccggttg atgcttgttg
     5521 atgaccacga aattgtgcgc accggcctgc gcacattcct ccaatct
//
LOCUS       DBNHOH010000043         3738 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_2094, whole genome shotgun sequence.
ACCESSION   DBNHOH010000043 DBNHOH010000000
VERSION     DBNHOH010000043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_2094"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="AC16V7_00695"
     CDS             complement(<1..591)
                     /locus_tag="AC16V7_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HIR6255663.1"
                     /translation="MAGQTQSPNGSRRWFAKIWSPWVRARIGSRWLLLIGDLLLIGVA
                     VMGSFGLRLEFGSRFIFFYPQALRMMLLAMLIKPLVYYIFGMYRMMWMYASVKEIRLA
                     ILSVSTGSVLLSIAVIVMTSLQSRLPGYLGFPRAVLVIDWLLSLILVGGLRFSLRILA
                     ELGSGPIEVKGHVRHVLIVGAGDAGALVVREMQKNPQ"
     gene            complement(593..2029)
                     /locus_tag="AC16V7_00700"
     CDS             complement(593..2029)
                     /locus_tag="AC16V7_00700"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR6255664.1"
                     /translation="MKSPNYLQKSHPWRLRLQERRTLLLMGDLIIAIISLVLALYFWG
                     IADRFPGWSLEFIQRRVPGWFFLFPIFWLILLVELYDVHRAGNWRATISGVATATLIG
                     FGLYLLLYFSYLDPPKSLLPRRGVAGFFIAAPLLTLIWRLFYIRIFTDPQFMRRVLLI
                     GGGKAGQTMLQIINDLWPPPFYVAGIIDDNVAKVGTTIENQQVLGTSESLLELIEHHN
                     VSDLIVAISGEMKSGMFQAMLEAQERGVEIIRMPKAYEDMLGRVPIRLLEADWILRSF
                     VDEHHVSGFYEMVKRIIDIIGGLVGVVMLILLMPFAAVAIWLDDGLPIFYTQFRAGKG
                     SQPYKILKLRTMRRDAEAAGVPQWAREDDARATRVGRLLRKSHLDELPQFINVLRGEM
                     SLVGPRSERPELMGVFQQSVPFYRARLLVKPGITGWAQVNFGYASDIEETTIKLEYDL
                     YYIKHRGLLLDLIIMLRTPATVFGFRGR"
     gene            complement(2016..2399)
                     /locus_tag="AC16V7_00705"
     CDS             complement(2016..2399)
                     /locus_tag="AC16V7_00705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR6255665.1"
                     /translation="MDPQIRTRYFARANLSALWKQYWRYGFWKVRMLRRYPQSLRWRQ
                     ALPPLFVFSLILFGIFSFWMPFFRWLLIFQLIIYSLVIILAGVRKALSKRYMPYVLGL
                     PLALSVMHLAWGSGALLSMLRYEKP"
     gene            complement(2418..3008)
                     /locus_tag="AC16V7_00710"
     CDS             complement(2418..3008)
                     /locus_tag="AC16V7_00710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6255666.1"
                     /translation="MPTVTIIVPCYNEQATIGLLLQAIYDQSYPRVQMEVVIADGLST
                     DQTLSQIQAFTQTHPDLKVRVVENPQRTIPSGLNQALRSALGDIIIRLDAHSIPYSNY
                     VAGCVAALQNQCGENVGGVWEILPGGSGWLAKSIAAAAAHPFGAGDARYRIGGLAQEV
                     DTVPFGAFRRTLIEKIGYFDESLLTNEDYEFNVRIQ"
     gene            complement(3186..3569)
                     /locus_tag="AC16V7_00715"
     CDS             complement(3186..3569)
                     /locus_tag="AC16V7_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR6255667.1"
                     /translation="MSQKEVIRTNNAPKAIGPYSVANRIGNLVFTAGQIGLDPTTNEL
                     VSGGIEAETRQALTNLKNVLDAAGSSLEQVVKTTVFLRDMGEFAQMNAIYAEFFPQNH
                     PARSTVQVAALPKYAAVEIEVVATI"
BASE COUNT         1081 a          961 c          837 g          859 t
ORIGIN      
        1 ttgcggattt ttttgcatct cgcgcacaac cagcgcacca gcatcgccag caccaacgat
       61 taatacatga cgaacgtgcc cttttacctc gatgggaccg ctacccaatt ctgccagtat
      121 tcgtaaagaa aaacgcaaac caccgactaa tatgagagaa aggagccaat caattaccaa
      181 aaccgcgcgc gggaatccga gatatcctgg cagacggctc tggagtgagg tcatgacaat
      241 gactgcaatc gaaagtaaaa ccgagccagt tgaaacagag agtatggcga ggcgaatttc
      301 ctttacactg gcgtacatcc acatcatgcg gtacatgccg aaaatgtaat acaccagtgg
      361 cttaatgagc atcgccagca gcatcatccg cagggcttgg gggtaaaaga agataaagcg
      421 agagccaaac tcgagacgaa gtccaaagct gcccatgact gccacaccaa tcaacaatag
      481 gtcgccaatt aatagcagcc accttgatcc aatcctcgcc cgaacccatg gtgaccagat
      541 tttagcaaac caacgacgcg agccgtttgg agattgtgtc tgacctgcca tcttatcgcc
      601 ctctaaatcc aaataccgtt gctggcgtcc gcaacataat aatcaaatcc agcagcagcc
      661 cgcgatgttt aatgtaatac aaatcgtatt ctaacttgat tgtggtctcc tcgatatcag
      721 aggcatagcc aaaattgact tgcgcccatc ctgtgatgcc aggtttcacc aacagtcggg
      781 cccggtagaa gggcaccgat tgctgaaaaa cacccatcag ctcgggtcgc tccgagcggg
      841 gaccgaccag gctcatctca cctcgtagca cattaataaa ttgaggtaat tcatccagat
      901 gggatttacg caataaacgc cctactcgcg tggctcgagc atcgtcttct ctagcccatt
      961 gcggcacacc tgcagcctct gcgtcccgac gcattgttcg taatttaaga attttataag
     1021 gctgggaacc cttgcctgca cgaaactgag tgtagaaaat tggcagacca tcatcaagcc
     1081 agattgcaac tgccgcaaaa ggcatgagta aaatcagcat cacaactccc acaaggcctc
     1141 cgatgatgtc tatgatgcgc tttaccattt cgtagaatcc gcttacatga tgttcatcca
     1201 cgaacgagcg gaggatccaa tcggcttcca gtaatcggat tggcacccgt ccaagcatat
     1261 cttcgtaagc tttgggcata cggattatct cgacaccacg ctcttgcgcc tccagcatgg
     1321 cttggaacat ccctgatttc atttcgcctg atattgccac aattaaatca gagacattat
     1381 gatgttcaat caattctaat aaagattcgc tggtcccaag aacctgctga ttctcgatgg
     1441 tcgtacccac tttggcgaca ttatcatcga ttattcccgc aacatagaaa ggaggaggcc
     1501 aaagatcatt gatgatctgt agcatggttt gaccagcttt tccacctcca attagaagca
     1561 cccgtcgcat gaactgcgga tcggtgaata tacggatata aaataaacgc cagatcaggg
     1621 tcaagagtgg cgcagcgatg aagaatccag ccacaccgcg cctcgggagc agtgattttg
     1681 gtgggtcgag gtaggaaaaa taaagcaaga gatatagccc aaaaccaatt aaggttgcag
     1741 tcgcaactcc gctgatcgtt gctcgccaat taccagcacg atgcacatcg tacaattcga
     1801 ccagtaagat taaccaaaag attgggaaca aaaaaaacca acctggaact ctgcgctgta
     1861 tgaattcaag cgaccaacct gggaagcggt cagcgatgcc ccagaaatac aaagccagca
     1921 ccaggctaat tatggcaata attaggtcac ccatcagcag caacgtacgg cgctcctgaa
     1981 gtcgtaagcg ccacggatga gatttctgca gataattagg gcttttcata tcgaagcatg
     2041 ctcaaaagcg caccgctacc ccaagccagg tgcatcaccg acaaggctaa aggtaatccc
     2101 aagacataag gcatataacg cttgctaagt gctttccgta cacctgccaa aataattacc
     2161 aagctataga tgatcagttg aaaaatcaaa agccagcgaa agaaaggcat ccaaaatgag
     2221 aagatcccaa ataggatcaa actaaataca aataatgggg ggagtgcttg cctccaccgt
     2281 aaggactgcg gataacgcct caacatgcga actttccaga aaccataccg ccaatattgc
     2341 ttccataacg cactcaaatt tgcgcgggca aaatagcggg tgcggatctg cggatccatc
     2401 catattttgc cccctgacta ctggatacgg acattgaact cgtaatcctc attggtcagg
     2461 aggctttcgt caaaataacc gatcttctcg atcaaagtac ggcgaaaggc gccaaagggc
     2521 actgtatcca cctcttgtgc taagccaccg atacgataac gcgcatcccc agccccaaat
     2581 ggatgcgccg cagcggcggc aattgatttc gctagccatc cacttccacc aggtagaatc
     2641 tcccacaccc cacccacatt ttcgccgcac tggttttgga gagctgctac acaaccagcg
     2701 acgtaatttg aatacggaat tgaatgcgcg tctaggcgga tgattatatc acccagtgca
     2761 gatctcaagg cctgattaag cccagaggga atggttcgct gcgggttttc gaccactcga
     2821 acctttaaat ccgggtgggt ttgtgtaaat gcttggattt gtgagagcgt ttggtcggtt
     2881 gaaaggccgt cagctatcac cacttccatt tggactcgtg ggtacgactg atcataaatc
     2941 gcctgcaaga gaagtccaat cgtagcttgc tcattgtaac atggcacaat aatcgttaca
     3001 gtgggcatgt ctctatgcct tgttgctcat gaaagactcg attgggtata ccaaggggta
     3061 cttaaccaca agaatactag ccatctatat tagtacttca cggcatcacc atgcaggttt
     3121 gtaaaaaata gcgataacaa ctttgcagca cgatatatta tgggatcagc tgtttaggct
     3181 atcgatcaaa ttgtcgctac aacctcgatc tctaccgcgg catatttggg taaagcagcg
     3241 acctgcactg tcgatcgtgc gggatggttt tgagggaaga attcagcgta gattgcattc
     3301 atctgggcaa attcgcccat atcgcgtaag aaaactgtgg ttttaaccac ttgctcgagc
     3361 gaagagcctg ctgcatccag tacattcttg agattagtca atgcctggcg tgtttcagct
     3421 tcaatgcccc cagaaaccaa ttcattggta gtcggatcta atccgatttg tccagccgta
     3481 aataccaggt tcccgatgcg gtttgccaca ctgtagggac caatggcttt cggcgcattg
     3541 tttgtgcgaa taacttcttt ttgggacata aatccacctc agatacttaa gttgatccat
     3601 tttaacatga aataccacga ccgaaacaga acacatgagc tacattaggg caatcgcatt
     3661 tgggcgattt cccaccattg aggagagaaa atggggtgtt ttggcggggg cataccccca
     3721 ccaaaacacc ccaaaaac
//
LOCUS       DBNHOH010000044         2488 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1475991, whole genome shotgun sequence.
ACCESSION   DBNHOH010000044 DBNHOH010000000
VERSION     DBNHOH010000044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1475991"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="AC16V7_00720"
     CDS             <1..274
                     /locus_tag="AC16V7_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6255668.1"
                     /translation="EEAVKAGLVNAVVPQDQVKPWAIEKAGHLAGLSRAACILVKQAI
                     QMGYGNWAAAMPEMEKLYLQELMATDDAHEGLAAFMEKRSPEWRHR"
     gene            292..750
                     /locus_tag="AC16V7_00725"
     CDS             292..750
                     /locus_tag="AC16V7_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HIR6255669.1"
                     /translation="MGSNRGLTFDEFEIGDRYQSQARTVTEADVVAFAGISGDFNPLH
                     TDAEFGKSTPFGERIAHGMLLVAMATGMANWTGVFEGTTIALMEQLIRYKGVVKFGDT
                     IHLELEVMEKTPTSKPDRGVVKYAARVINQRGEVVIDGVWTLLMKRQINQ"
     gene            821..1588
                     /gene="fabG"
                     /locus_tag="AC16V7_00730"
     CDS             821..1588
                     /gene="fabG"
                     /locus_tag="AC16V7_00730"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008604896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HIR6255670.1"
                     /translation="MKLAEKVVLITGGANGIGRATALRFAREGARVVVSDVDAEGLAT
                     VCQEVEQAGGQAISAVGSVTDRSQVQDIVDTALNTFGRLDILINNAGINRDALTTRIK
                     DDEVRFMSDENWDLVVNVNMRGTWLCSQIASVPMMKQKFGRIVNTSSIGALGNIGQAN
                     YAASKAGVIGLTKTLALEWARFNIAVNCVAPGATKTRMTAGIPEKLLEGLVAKIPFGR
                     LAEPEEIASAHLFFASNDADYITGQVLFVDGGISLGV"
     gene            1609..2391
                     /gene="fabG"
                     /locus_tag="AC16V7_00735"
     CDS             1609..2391
                     /gene="fabG"
                     /locus_tag="AC16V7_00735"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HIR6255671.1"
                     /translation="MTSEQSGLSPAPRLDLADKVAIVTGGSRGIGQAIAWDLACNGAH
                     VALNYRRSADEAQQLIQQIQGLGRKGLAIQADVASFADAQRMVETVVRELGSLDILVN
                     NAGVNRDSVIWKMSEEQWDEVISIDLKGTFNYIRAAAPIFKEQGSGKIVNVTSINGLR
                     GKFGQSNYTAAKGGVNALTKTVARELGRYNVNVNAVAPGLIETDMVKQAPQQVRDLAL
                     SEIVLGRLGTPEEVAWIVTFLCSERARHITGEVITVDGGQYL"
BASE COUNT          629 a          611 c          744 g          504 t
ORIGIN      
        1 cgaggaagcc gttaaggccg ggcttgtgaa cgcagtcgtc ccacaagacc aggtgaaacc
       61 atgggctatt gaaaaagctg ggcatctggc tggtctcagc cgggcagctt gcatcctggt
      121 caaacaagct atccagatgg gatacggcaa ttgggcagcc gccatgcccg aaatggaaaa
      181 gctctacttg caggaattaa tggcaaccga cgatgcgcat gaagggctgg cagctttcat
      241 ggaaaaacga tctcccgagt ggagacatcg atgagattta tgggagggta aatgggctca
      301 aatcgcggac tgacctttga cgaatttgaa atcggtgatc gataccaatc tcaagctcgc
      361 acagtgaccg aagccgatgt agtcgccttc gcgggtatat cgggggattt caaccccctt
      421 cataccgatg cagagttcgg taagagcaca ccctttgggg aacggatcgc ccatggcatg
      481 ctgctggtgg cgatggcgac cgggatggca aactggacag gcgtgtttga aggcaccacc
      541 attgccctaa tggaacagct catcagatac aaaggcgtgg tgaaatttgg cgacacgatc
      601 caccttgagc tagaagtgat ggaaaagacc cctacatcca aaccagatcg aggggttgtc
      661 aagtacgctg cgcgggtgat caaccagaga ggtgaagtgg ttatcgatgg cgtgtggacc
      721 ctgctaatga aaagacaaat caatcaatga aaactttgaa gcccgagcag taaatcagtg
      781 aattgacgtt tcaattcttt aagaaaatgc ggagggaaaa atgaagcttg cggaaaaagt
      841 cgttttaatt acggggggcg ccaatgggat tggccgggca actgccttgc gcttcgcccg
      901 tgaaggggca agggtggtgg tcagcgatgt agacgctgaa ggtctggcaa ctgtctgcca
      961 ggaggtcgaa caagccggcg ggcaagccat atctgccgta ggcagtgtca ccgaccgatc
     1021 ccaggttcag gacattgttg atacagcctt aaatacattt gggcgcttag acatcctgat
     1081 taataatgcc ggcatcaatc gcgatgcgct caccacccgc atcaaggatg atgaagtgcg
     1141 cttcatgtcg gatgagaact gggacctggt ggtgaatgtg aatatgagag gcacctggct
     1201 gtgctcacag atcgcctcag tgccgatgat gaagcagaaa ttcggtcgca tcgtgaatac
     1261 gtcttcaatc ggcgcgctgg gtaacatcgg acaggccaat tacgccgcct ccaaggcggg
     1321 cgtgatcggt ctaaccaaaa cacttgcgct ggagtgggcg cgtttcaaca tcgcggtgaa
     1381 ctgtgttgcg ccaggtgcga ccaagaccag gatgaccgcg ggtatccccg agaagctctt
     1441 ggaggggctg gtagccaaga tccccttcgg caggctggct gagcccgaag agatcgcctc
     1501 cgctcacctg ttcttcgctt caaacgatgc cgactacatc actggtcagg tcctgtttgt
     1561 agatggcgga attagtctgg gcgtgtaacg ctggaaggaa aagataacat gacatcagaa
     1621 caatccggat tgtctccagc tcccaggctg gatttagccg ataaagtcgc aattgtcacc
     1681 ggtggaagcc gtggcattgg gcaagccatt gcctgggatc tggcttgcaa tggggcgcat
     1741 gtagccctca actaccggcg gagcgccgat gaagcgcagc aactgatcca gcaaatccag
     1801 ggtctcggac gaaaaggtct tgcgattcag gctgatgtgg cttcttttgc agatgcccaa
     1861 cgcatggtcg agacggtggt gcgagaactc ggtagcctgg atatcctggt gaacaacgcc
     1921 ggcgtaaacc gtgacagtgt catctggaag atgagcgagg agcagtggga cgaggtgatt
     1981 tcgattgacc tgaaaggcac cttcaactac atccgggcag ctgcgccgat tttcaaggag
     2041 caaggctcgg gaaaaatcgt caacgtcaca tcgatcaacg ggttgcgcgg taaatttggg
     2101 caatcgaact acaccgcagc aaaaggagga gtgaacgcgc tgacaaaaac tgtggctcgc
     2161 gagctgggtc gctataacgt gaatgtcaac gcagtcgcgc caggattgat cgaaaccgac
     2221 atggtcaaac aggccccgca gcaagtgcgc gatttagcgc tgtcagagat cgtattaggt
     2281 cgcctgggca cgcctgaaga ggtagcctgg atagtcacct tcctgtgcag cgagcgcgcc
     2341 cgccatatta ccggggaggt cataactgtg gatggaggcc aatacctatg aaagaagcag
     2401 taattgtcgc tgctgtgcgc acacccatcg gcgcacatgg cggcgcctta agcgctgtac
     2461 gccccgacga catggcagct cttgtcat
//
LOCUS       DBNHOH010000045         4382 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_258344, whole genome shotgun sequence.
ACCESSION   DBNHOH010000045 DBNHOH010000000
VERSION     DBNHOH010000045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_258344"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..453
                     /locus_tag="AC16V7_00740"
     CDS             193..453
                     /locus_tag="AC16V7_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255672.1"
                     /translation="MKHRHRGDELSLENAVLLAVNACYDPGWSEEETESLLNEIASLR
                     LIEEGTKDSQPCDEEGRYAELEQKHFGDPEKETGIYAPKKHD"
     gene            631..972
                     /locus_tag="AC16V7_00745"
     CDS             631..972
                     /locus_tag="AC16V7_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255673.1"
                     /translation="MSDNNTLALDGLFTRSRAALSNFQGFGHCQSDTEDEHDGKARWY
                     WYFTADCDFGDGKPVKSRRVFMDQVVYYSDGDEYPDAHKNMEALSKACMAYLSANGYW
                     HKGSTWMPRAA"
     gene            1328..1936
                     /locus_tag="AC16V7_00750"
     CDS             1328..1936
                     /locus_tag="AC16V7_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255674.1"
                     /translation="MAAKKSTKLCECGCGNFTNIASKTQSSKGHIQGTPQRLLPGHNK
                     THGHCRGRKTPEYNAWRSMKKRCLLKTYFQYKFYGGRGISICERWMTFENFLADMGKR
                     PDGLSLERTDNEKGYSADNCIWATPAKQANNRRSNHVIEHAGITMTMTQWANKYSITV
                     QLLYGRLKRGWSMERALTAPAQVKTYRSRTTGLFSQNRSVGT"
     gene            complement(1889..2188)
                     /locus_tag="AC16V7_00755"
     CDS             complement(1889..2188)
                     /locus_tag="AC16V7_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255675.1"
                     /translation="MIDPVTLKALASGGTGAAIPIVMMYALFTPNQDFTSHVAESLRG
                     GILDLVAQAHDEEPGPFKDALCKSLEQQIAELCTSAPNDSLCTDRSILREKAGCS"
     gene            complement(2478..2873)
                     /locus_tag="AC16V7_00760"
     CDS             complement(2478..2873)
                     /locus_tag="AC16V7_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006033275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3TM-type holin"
                     /protein_id="HIR6255676.1"
                     /translation="MGILQKIIGNAGTGAIESIGGVMGKVFENKQEKLSHEEVMARLA
                     QNPQSWQAEINKLEAQHRSIFVAGWRPFIGWVCGSGLAFVFLFNPIIQWTTGKDGPQM
                     ATEALMTLVVSLLGLAGYRTVEKLQGRSK"
     gene            complement(2874..3215)
                     /locus_tag="AC16V7_00765"
     CDS             complement(2874..3215)
                     /locus_tag="AC16V7_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004918418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase domain-containing
                     protein"
                     /protein_id="HIR6255677.1"
                     /translation="MTLGQKQKLFASMVPRLIDKAHELGFEVTLGDAYRDPRSHGKVG
                     EKIAYGNAYSCHKTRLAIDLNLFKDGKYLTTTEDHRPLGEWWESRHELACWGGRFDDG
                     NHYSFTHEGMR"
     gene            complement(3212..3532)
                     /locus_tag="AC16V7_00770"
     CDS             complement(3212..3532)
                     /locus_tag="AC16V7_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255678.1"
                     /translation="MVILALILGWFVGLWAFVAVMHAKARYSDLTLFWKINLAPLAFI
                     GLIVDWLVFNWVFGTIMFLEVPKELLLTSRVKRHYRESKGWRLRVATWWRRNLNIIDP
                     GHIR"
     gene            complement(3526..3987)
                     /locus_tag="AC16V7_00775"
     CDS             complement(3526..3987)
                     /locus_tag="AC16V7_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255679.1"
                     /translation="MKKRDIGIALDNLTEDVKTQTLWLVQSYRDSAGAFNQTDLIKQA
                     VDSPARARAQVQQWIVAYNAKNGAGTAQVFLAECLTAAGSVKTVQSIDAELSGLQAQA
                     QTVVNNVAGGWTWDQAATAIENAIVVQTDEDFSYLQLPVPDGYITVWGEPW"
     gene            complement(3984..>4382)
                     /locus_tag="AC16V7_00780"
     CDS             complement(3984..>4382)
                     /locus_tag="AC16V7_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255680.1"
                     /translation="TLNVVGQVQFSGASSFTTDTWTGFSSAPSGDLNYFDMGTHVMVW
                     NDAAKTGTSNANTFTVGTLPSAIRPTAQRNVPCLAVDNSGSVVANAAVNTSGLISMSA
                     PDAAADWGTTNWTTSANKGLAGGWIIFYAK"
BASE COUNT         1149 a         1167 c         1038 g         1028 t
ORIGIN      
        1 gagcaggcta ggcgggttga ggctgagagg cggttaaacg agattgccca actgtctaat
       61 gggctgacac cgagcaatga tgagcgccag ttactttacg acatctatgc tgtgacccaa
      121 ggccatacca aatggacaga acccacagag gaaggcactg aacaatgact gaccagacca
      181 accgagaaaa gtatgaagca tagacatcgt ggtgacgagt taagtttaga gaatgctgtt
      241 ctattggcgg ttaatgcatg ttatgaccca ggatggtcag aagaagaaac cgaatcctta
      301 ctgaacgaaa ttgcaagcct aagacttatt gaggaaggca ctaaagacag ccagccatgt
      361 gatgaagaag gtcggtatgc agagttagaa cagaagcatt ttggcgatcc cgaaaaagaa
      421 acaggaatct acgcaccaaa aaaacacgat tagttgcgcc ttcaataact tacgaaaggc
      481 gttcccacca gcaaacaatc acttacattc gttaagtggt taataaataa ccagaaaaca
      541 cggtttgata taatggtagt tgagccgatc aggtggtttg ctcccccggc ggacaccggg
      601 gttgctcttt aacaagatga ggaaatcaac atgagtgata ataacacgct cgcgctagat
      661 ggtctattta cacgatcaag agccgcgctc tcgaacttcc aaggcttcgg ccactgtcaa
      721 tcagacacgg aagatgagca cgacggcaag gccagatggt actggtactt caccgctgac
      781 tgtgatttcg gagacggcaa gccagtaaaa tctcgtcgtg tgttcatgga tcaggtcgtc
      841 tactactcag atggtgacga gtaccctgac gctcacaaga acatggaggc tcttagcaaa
      901 gcctgcatgg cttacctatc tgccaatgga tactggcaca aggggagcac atggatgccg
      961 cgagcggcgt aacaaactgg gggagggctt cggctctccc ctttttttat aaacatccat
     1021 ccgtataacc aacagaggaa tgaccatgag caacatcaag ccaagcgaat ttgagcagat
     1081 gagacaggat atggaaaatc gtgaattgcg cgttccgtgg tctgataccg gatgcaaatg
     1141 cacgttcgct cagagattgg gtggtgacgg ttgccagaag tgccagcccg cgtcagagag
     1201 aaacaaggac ggcactaaag acagccagcc ttctccagaa acacctgacg atctataatc
     1261 gttgaagccc ggagagcgtc aactctaccg ggcctctgac caagccaaac ctgaaaggag
     1321 gtccgtcatg gctgcaaaga agtctaccaa actgtgtgaa tgcggatgcg gcaactttac
     1381 caacattgcg tctaaaacac agtccagtaa ggggcatatt caaggaacgc ctcagagatt
     1441 gttgccggga cataacaaaa cccacggcca ttgcagaggc agaaaaacac ccgagtacaa
     1501 cgcatggcga agcatgaaaa agcgctgcct actaaaaact tatttccagt ataagtttta
     1561 tggtggtcgt ggaatttcca tctgcgaaag atggatgacc tttgagaact ttctggctga
     1621 tatgggaaag cggccggatg gcctttcttt ggagcgaacc gataacgaaa aagggtactc
     1681 agcagataat tgtatctggg caaccccagc taagcaggcc aacaatagac gatctaacca
     1741 cgttatcgag catgccggaa taacaatgac aatgactcaa tgggcgaata agtattctat
     1801 aacagttcag ttgttatacg gcagacttaa gcgaggctgg tctatggagc gcgcacttac
     1861 tgctcctgct caagtaaaaa cataccgatc aagaacaacc ggccttttct ctcaaaatcg
     1921 atctgtcggt acatagacta tcgtttggcg cactggtaca caattcagct atctgctgct
     1981 ctagactctt gcaaagagcg tccttgaacg ggccgggttc ttcgtcatgc gcttgagcca
     2041 ccaaatctaa aataccgccc cgcagtgatt cggcaacgtg cgaagtgaaa tcctgattcg
     2101 gcgtgaacag ggcatacatc atcacgatgg gaatagccgc accagttccg ccactggcta
     2161 aggctttcaa tgttacaggg tcaatcacgg taatctcccg ttcagtcgag cctctagttt
     2221 gtcgaatgag tggtctatct tgtcggctaa ccctttgtga tcggccttaa cttccaacct
     2281 caacgaatcc accttgcctt cgatcttttc gtgatacctt gcattgtctc tagcgtgtcg
     2341 atcaacgcgg gattctagtc gagttattcg acgcatgagc aacgcccata tcgtcccgag
     2401 taatgtcgcc aggacgccaa tggaccaatt tgcgatatgt gtaacccagt ccaaacattt
     2461 gtcctcaggc tcccggccta tttcgatcta ccctgtagct tctcgacggt tcgataaccg
     2521 gccaagccta acagggacac taccaatgtc attaacgcct cagtagccat ctgcgggcca
     2581 tccttgcccg ttgtccactg aatgatgggg ttgaacagga acacgaaagc cagtcccgat
     2641 ccacaaaccc agccaatgaa cggtcgccac ccagcaacga agatggacct gtgctgggct
     2701 tccagcttgt tgatttccgc ctgccacgat tgcgggtttt gggcgagtct cgccatcact
     2761 tcttcgtgtg acagcttctc ctgcttgttc tcgaacacct tgcccattac gccaccgata
     2821 gactcgatag ccccagtgcc ggcgttacct atgatcttct gcaaaattcc catctatctc
     2881 atcccttcgt gagtgaacga ataatgattt ccgtcatcga aacggccacc ccagcaagcc
     2941 agttcgtgac gcgactccca ccactcacct agtggcctgt ggtcctctgt ggtggtcaga
     3001 tacttcccat ctttgaacag attcaagtct atcgccaatc gggtcttgtg gcagctatag
     3061 gcgttcccgt aagcgatctt ctcacccacc ttaccatggg atcttgggtc tctataggca
     3121 tcccctagcg tgacctcgaa ccccagttca tgcgccttgt cgatcaggcg agggaccatg
     3181 ctggcgaata gcttctgctt ctggccgagc gtcatcggat atgccctgga tctattatgt
     3241 tcaagttcct gcgccaccaa gtcgctaccc taagtcgcca gcccttagac tcccgatagt
     3301 gccgcttaac ccttgaagtc agcaatagct ctttaggaac ctctaggaac atgatggtgc
     3361 cgaacaccca gttgaatacc agccagtcta cgatcaggcc gatgaaagcc agcggagcta
     3421 ggttgatctt ccagaataag gtgaggtcgc tgtaccttgc cttggcgtgc atcaccgcca
     3481 caaaggccca cagaccaaca aaccaaccga gaatgagagc aagtattacc acggctctcc
     3541 ccatacagtg atatagccgt ccggtactgg tagctgtaga tacgaaaagt cttcgtcagt
     3601 ctgtacaaca atcgcgtttt ctatcgcagt cgctgcctga tcccaagtcc atcctccggc
     3661 tacgttattc accacggtct gtgcttgcgc ctgtaatccc gatagctctg cgtcaatgct
     3721 ctgaactgtc ttgactgatc ccgctgctgt caagcattcc gcgagaaaca cttgagctgt
     3781 accagccccg tttttcgcgt tgtacgcaac gatccattgc tgaacttgcg cccttgccct
     3841 ggccggcgaa tcgacagcct gctttatgag atcggtttga ttaaacgctc ctgccgaatc
     3901 acggtacgac tgcaccaacc acaaggtttg tgttttgaca tcctctgtca aattatcgag
     3961 agcaattcct atgtcacgct ttttcatttt gcgtaaaaaa taatccagcc accagctaga
     4021 cccttattag cagaagttgt ccaatttgtc gttccccaat ctgccgctgc atccggtgcg
     4081 ctcatgctta tcaagccact ggtatttaca gcagcatttg ccacgaccga tccgctgtta
     4141 tctaccgcca agcatgggac gttccgttga gcggttgggc gaattgccga tggcaacgta
     4201 ccgactgtaa acgtattggc attcgatgtg cctgtcttgg ctgcgtcgtt ccataccatg
     4261 acatgcgtac ccatatcgaa ataatttaag tctcccgatg gagcagaact aaatccagtc
     4321 catgtgtcgg tagtaaagct actcgctcca gaaaactgca cttgcccgac cacattgagc
     4381 gt
//
LOCUS       DBNHOH010000046         3589 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1347997, whole genome shotgun sequence.
ACCESSION   DBNHOH010000046 DBNHOH010000000
VERSION     DBNHOH010000046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1347997"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="AC16V7_00785"
     CDS             <1..993
                     /locus_tag="AC16V7_00785"
                     /inference="COORDINATES: protein motif:HMM:NF020067.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR6255681.1"
                     /translation="ETSRLGSVSHRRQNRRDQGIDQRLREKVREAKARGSSYEKRRSP
                     PVMVRGGIAGPPLPLRKTTNSRAKRRYDLTLSVPGAEMRLPALPQISIDLRLLSGVMV
                     AVLGLLIYYLWNSPSFHVEGAKIIGLQRLNSRDVNAIMNLEGESIFAIDPQVLEQKAR
                     EAFPEFALVKVDVGMPANVDITVEERLPILTWRQEGRTVLVDANGIAFPQRDQGDSTP
                     AMVVEAFDSPPIMQNESALAGSPVQFIPVEMVSAILSMSAQAPDETPVVYDSKHGLGW
                     KDTRGWEVYFGDALDIDMKLRVYNAMLKELQDDGVQPVLISVEYIHRPYYRLER"
     gene            1029..2285
                     /gene="ftsA"
                     /locus_tag="AC16V7_00790"
     CDS             1029..2285
                     /gene="ftsA"
                     /locus_tag="AC16V7_00790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIR6255682.1"
                     /translation="MEQPIVVGIDVGTTKICTLVGRENAPGQLSILGVGIEPSQGIRK
                     GVVVDLAAASQAVARSIEKAERTSGLEITSGLVSMAGSQVTSSNNRGVVGISGRVIDQ
                     EDTERAIDAARAVALPHNREIIHVIQRGFNVDGQDGIRNPIGMHGYRLEVETHIITAS
                     EASVENLRQCVGISGVEVSQFVLNPLASAEVVLNETERQMGCVVVDMGGGTTDMAIFI
                     DGDVWYTAVIGVGGNHITSDIAHGLRLPMSQAEEIKIQHGHAVQAQVGAQESFNVKPF
                     GEQDSLQINRQELAHIIEARVEEIFSLVLQEIKRSGYDGLLPAGMVLTGGCSALPGVR
                     DVASQVLGVSARIAQPENLVGMTDRLNSPAFSTSVGLLRWALMMSEYSPQGVKRQKSA
                     PRVAAGAQGGAFNWENIKIWLRRLLP"
     gene            2386..3510
                     /gene="ftsZ"
                     /locus_tag="AC16V7_00795"
     CDS             2386..3510
                     /gene="ftsZ"
                     /locus_tag="AC16V7_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR6255683.1"
                     /translation="MKHETNLPNQIESFARIKVVGVGGGGCNAVDRMIDEGLQGVDFV
                     AVNTDAQALMLSKAPRRVRVGEKLTRGLGSGGDPETGRKASEESAEELYEVLRGSDMV
                     FITAGMGGGTGTGGAPIVSQIAKEVGALTIGVVTRPFTFEGARRIQSAESGITKLKEQ
                     ADTLIVIPNDRLLQIVDKRANLQQAFRMADDVLRQGVQGISELITVPGLINLDFADVR
                     TIMSEGGAALMAVGMGKGEDAARIAAEQAISSQLLDITIDGARGILFNVTGGPELTLF
                     EVNQAAAIIKETAHPDVNLIFGAVIDQSLKDEVHITVIATGFERTGVPRRIVEPAATE
                     VHPSATPASIPVGQPAREFQPRAFNTEDLDIPTFLRNRGR"
BASE COUNT          813 a          901 c         1066 g          809 t
ORIGIN      
        1 gaaacgagtc gcttgggatc agtaagccac cgaagacaaa atcgccggga tcagggtata
       61 gaccagcgtc ttcgagaaaa ggttcgtgaa gccaaggcgc gcggttcctc gtatgagaag
      121 cgtcgctctc cgccagtgat ggtgcgaggt ggaattgctg ggccgcccct gcctttgcgc
      181 aaaaccacga atagccgggc aaagcgccgc tatgatttaa ccctgagtgt gcctggtgcg
      241 gaaatgcgcc tcccagctct accgcaaata tcgattgatt tgcgcctgct ctcgggcgtt
      301 atggtagcgg tattaggatt gctcatatat tatctatgga attctccttc atttcacgta
      361 gaaggtgcta aaattatcgg gttgcaaagg ttgaacagcc gcgacgtcaa cgccatcatg
      421 aatttggaag gtgagtcgat ctttgccatc gacccccagg tgttggagca gaaagcccgg
      481 gaagcattcc cggaatttgc cttggtcaag gtggatgtag gcatgcctgc gaatgtggac
      541 atcactgtcg aagaacgcct gccaattctg acctggcgac aggaagggcg tacggtacta
      601 gtggatgcta atggaatcgc tttcccccag cgcgatcaag gagattctac cccagcgatg
      661 gtggtggaag ctttcgattc accgcctatt atgcaaaatg agtcagccct agcgggcagc
      721 ccggtgcagt ttattcctgt tgagatggtt tctgcgatcc tttcgatgag cgcccaagcg
      781 cccgatgaaa ccccagtggt atacgattcc aagcacggtt tgggctggaa agatacccgg
      841 ggctgggaag tgtacttcgg agatgcgctt gatattgata tgaaattgcg cgtctataat
      901 gcgatgctca aagaactaca agacgatggc gtgcagccag tcttgatcag cgtggagtac
      961 attcacaggc cttactaccg cctggaaaga taaacgctgc cagtgttata gaatggagcg
     1021 agctactgat ggagcaaccg atagttgtcg gaattgatgt aggtacaacc aagatttgta
     1081 cgctggtagg gcgagagaat gccccaggcc agttgagtat cctgggtgta ggaattgagc
     1141 cctctcaggg aatccgcaag ggcgtagtcg tcgatcttgc agctgcttcg caagcggtag
     1201 ctcgttcgat agagaaggcc gagcgtacat ctggcttgga gatcacatct ggcttggtta
     1261 gcatggctgg ctcccaggtg acttcatcaa ataatcgtgg tgttgttggg atcagcgggc
     1321 gggtgatcga tcaagaagac acagagcgcg ccatcgatgc tgcccgagct gtggctttac
     1381 cgcataatcg cgagatcatc catgtcatcc agcgtggctt caatgtggat ggccaggatg
     1441 gcattcgcaa tccgattggc atgcatggat accgtctgga agtggagacg catatcatca
     1501 ccgcctcaga ggcatcggtc gagaacttgc gccagtgcgt tggtatctca ggggtcgagg
     1561 tctctcaatt cgtactaaac ccactcgcat ctgctgaagt cgtgctcaac gagacagagc
     1621 gtcaaatggg ctgtgtggtg gtcgatatgg gcggtgggac cacggatatg gcgattttca
     1681 tagatggcga cgtgtggtat acagccgtga tcggtgtggg aggcaaccac atcacatcgg
     1741 atatcgccca cggtctgcgc ctgcccatgt cacaggcaga ggagatcaag atccagcacg
     1801 ggcatgccgt ccaggctcaa gtgggagcgc aagaatcatt caatgtaaaa ccattcggcg
     1861 agcaagacag tttgcagatc aaccgccagg agctagccca tatcatcgag gctcgcgtgg
     1921 aagagatctt ctcgctggtg cttcaggaga tcaagcgctc tggttatgat ggcctgctgc
     1981 ctgccgggat ggtgctcacc ggaggctgca gcgccttgcc aggtgtacgc gatgtcgcct
     2041 cgcaggtact gggtgtgtcc gcccgcatcg cgcagccgga gaatctggtc ggcatgaccg
     2101 accggttgaa ctcaccagcg ttctcgacca gtgtaggttt gctgcgctgg gcgctgatga
     2161 tgagcgagta ctcccctcag ggggtaaaac gtcaaaaaag tgcgccacgc gttgccgcag
     2221 gggcgcaggg aggtgcattc aattgggaaa atatcaagat ttggctgaga aggttattgc
     2281 cttgagtcct atgacaagag attttcaatt catcaatcgt cgaacatcaa catatctatg
     2341 caaatcttta acggataaat caattttata gaggaggaac ttgtaatgaa acatgagacc
     2401 aacttgccca atcagataga atccttcgcc cgtattaaag ttgtaggcgt tggcggaggc
     2461 gggtgtaatg ccgtcgatcg catgatcgat gaaggcttgc agggcgtaga ttttgtggca
     2521 gtgaacaccg atgctcaggc tttgatgctt tccaaggcgc ctaggcgtgt acgtgtgggc
     2581 gaaaaactca cccgcggcct gggttcgggc ggagaccccg agaccggacg aaaagcctcg
     2641 gaagagtcgg cggaggagct gtacgaggtg ctgcgcggct cagacatggt gtttattacc
     2701 gcaggcatgg gtggaggcac aggtactggc ggagcgccaa tcgtttccca gattgccaag
     2761 gaggtcggtg ccctcactat tggtgtggtt actcgcccgt ttacttttga aggtgcgcgc
     2821 cgcatccaat cagccgagtc tggtatcacc aagctgaaag aacaggcgga taccctgatt
     2881 gtcatcccga acgaccgctt gctgcaaatc gtggacaagc gcgccaacct gcaacaggct
     2941 ttccgcatgg cggatgatgt gttacggcag ggtgtccagg ggatctcaga gctgatcacg
     3001 gtaccagggt tgatcaactt ggactttgcc gatgtgcgca ccatcatgtc ggaaggtggc
     3061 gcggctttga tggcggttgg tatgggcaaa ggtgaagatg ctgcccgtat tgccgctgag
     3121 caagccatct ccagccaatt gttggacatt accatcgatg gcgcccgcgg catcctcttc
     3181 aacgtgaccg gtgggcctga gttgaccctc ttcgaggtga accaggcggc tgccattatc
     3241 aaggaaaccg cccacccaga tgtgaacctg atctttggtg ccgtcatcga tcaaagcctg
     3301 aaggacgagg tgcacatcac ggttattgct accggtttcg agcgcactgg cgtgcctcgg
     3361 cggattgtag agcctgcagc taccgaggtg catcccagtg ccacgcctgc ttcaatcccg
     3421 gtgggccagc cggctcgcga attccagcca cgagctttca acaccgaaga cctggatatt
     3481 ccgaccttct tgcgaaaccg cggccgctaa ttgcggatgc ctggtttcac catttatagt
     3541 gtttcagcca acaactgccg gactcctcct cccggtcgtt gttgggttt
//
LOCUS       DBNHOH010000047         1176 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1412230, whole genome shotgun sequence.
ACCESSION   DBNHOH010000047 DBNHOH010000000
VERSION     DBNHOH010000047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1412230"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1176)
                     /gene="ligA"
                     /locus_tag="AC16V7_00800"
     CDS             complement(<1..>1176)
                     /gene="ligA"
                     /locus_tag="AC16V7_00800"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIR6255684.1"
                     /translation="TMNISDVDPSLRDRIENLRREIHYHNYRYHVLDSPLISDYEFDQ
                     LMLELRQIEEEHPDWITTDSPTQRAGSVPSEKFIKVRHPAPILSLGNAFDADGVRAWF
                     DRILKLDERVIQADFVVEPKIDGLTVVLHYRNGIFVQGATRGDGEIGEDITANLRTIR
                     ALPLRIPISVDGPMPPSYLVVRGEAFITPADFEALNRQLEERGEKTYQNPRNTAAGAL
                     RQLDPALTATRPLTLLTYDVVDGDGSLSESHTETLGMLRALGFPVIDEEHCDSLDDAI
                     QAYNRLVERRDQLPFEADGVVIKINDLGLGENLGVVGKDPRGAIAFKFPAREVTTTLM
                     GIGVNVGRTGVLTPYAILEPVEIGGVIVRQATLHNFDYIADKDIRIGDRVAVKRAGD"
BASE COUNT          306 a          316 c          269 g          285 t
ORIGIN      
        1 catcacccgc acgcttgact gctactcggt caccgattcg tatatctttg tcggcgatgt
       61 aatcaaaatt gtgcaatgtg gcttgacgga caatcacacc cccgatctca accggttcca
      121 ggatagcata gggagttaat acacctgtac gaccaacatt aacaccgata cccattaaag
      181 tggtggtcac ctcccgcgcg ggaaacttga acgcgatggc accgcgtggg tccttgccaa
      241 ccacacctaa attttctccc aaacccaggt cattaatctt gataactaca ccatctgcct
      301 caaaaggaag ttggtctctt cgctcaacta atcggttgta tgcctggatg gcatcatcta
      361 ggctgtcgca atgctcctca tcaatgacag gaaagcccaa ggcacgcaac atacctagtg
      421 tttcggtgtg actctcagaa aggctgccat ccccatcaac cacatcatag gttagcagcg
      481 tcaaaggacg agtagcagtt agagctggat cgagctgccg caaggctcct gcagcagtat
      541 tgcgcggatt ctggtaggtt ttttcgcccc gctcttctaa ttgtcgattt agggcctcaa
      601 aatcggccgg agtgataaaa gcttcaccac gcacaaccaa ataggatggg ggcatgggcc
      661 catctacgga tatagggatt ctcaagggca gagcacgaat tgttcgcagg ttagctgtga
      721 tatcttcgcc gatttcgcca tcgccgcgcg tcgcaccttg cacaaaaatc ccattgcggt
      781 aatgcaagac cacggttaat ccatcaatct ttggctccac aacgaaatca gcctgaatta
      841 ccctctcgtc cagcttcaag atacgatcaa accaggcacg cacgccatcc gcatcaaaag
      901 catttccaag actcaaaatg ggtgctgggt gtctaacctt aataaatttc tctgatggga
      961 ctgagcctgc acgttgggtc ggtgagtcag tcgtgatcca atctggatgc tcttcttcga
     1021 tttgccgaag ctccaacatc agttggtcaa actcgtaatc actgatcaat ggggaatcta
     1081 atacatgata ccgataattg tggtaatgaa tctcgcggcg aagattctcg atgcgatcgc
     1141 gcaggctcgg gtcaacatca gagatgttca tagttc
//
LOCUS       DBNHOH010000048         2399 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1732660, whole genome shotgun sequence.
ACCESSION   DBNHOH010000048 DBNHOH010000000
VERSION     DBNHOH010000048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1732660"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1253)
                     /locus_tag="AC16V7_00805"
     CDS             complement(75..1253)
                     /locus_tag="AC16V7_00805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6255685.1"
                     /translation="MKLLVISHTPHYIQDGHIVGWGPTVREIDYLARMFDQVVHLAVL
                     YKNDTPSSSLPYQSSNVKFVPVRPAGGDSFRAKMDVIRATPEYLRAIGKTLSEFGQND
                     IVHVRCPSNISLIASQRMAINNRVPHRWVKFAGNWAALGTGSWSYSFQRWLLRNNLHS
                     GVVSVNGRWPSQKPHIFSFYNPSFTDEEVLQASLLGRNKQLEQPLQLLFVGRMDKAKG
                     LDRILKIACMLKTHNIRFELNLVGDGPEKETLQAMVADCNLQHDIHFIGWLRRAEIST
                     YYARAHFFLLPSAAEGWPKVLSEGMAYGVVPLASTISSIPQILAEFGCGRVIPPQDLE
                     GYVQAMIDYLDHPEIWLAESHAGIAAARHFTYVSFQESLKAMALEAWGLHINPSIDQP
                     "
     gene            complement(1250..>2399)
                     /locus_tag="AC16V7_00810"
     CDS             complement(1250..>2399)
                     /locus_tag="AC16V7_00810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6255686.1"
                     /translation="GPGGIGTHAYQMALQMSMSHWRVLVLAYQDYMSHSEIEEFNHTQ
                     PFEIIQLQRQKHQAADLRFRWEQLQQTVIKWKPDLLVVTGDRLVWLTAAAQAVRGLLD
                     RTGIKLPWCAIWHGIHPSNPFQKLMMKWAYSKPDLTIAVSRYSKSHLLSLGVQSSRVA
                     VIPNGANDKVFFPDVSSGQTLRTQMGFADAFVLLTVGHVSHRKGQDIVIRSLPEVLER
                     NPRTYYLIAGLPTMQPELEKLVNALGIGNHVHFMGRAQQEELFAAYNACDVFVLVSRH
                     SRDGEYEGYGIVVSEAALCGKPSVVAENSGLTETVSDGVTGFVVPENDPHTTAQTILA
                     LMENSELRKKMSLEARQRAITEQTWEVSMDRYNKAFRQLLLDSGGRRS"
BASE COUNT          626 a          598 c          554 g          621 t
ORIGIN      
        1 tttatccaga taaaattgag gtcgattgaa aaatgtttga acctgctcgg tttggtccac
       61 catgaaaatc acagttatgg ctgatcaatg gacggattaa tgtgaagtcc ccaagcttcc
      121 aaggccatgg ctttcagcga ttcttggaaa gaaacgtatg tgaagtgccg ggcagcagca
      181 atcccagcgt ggctttctgc cagccagatc tcagggtgat ctaaataatc aatcattgcc
      241 tgcacatagc cttctaggtc ttgaggagga atcactcgtc cgcagccaaa ttcagccaga
      301 atttgcggga tacttgaaat cgtgctcgcc aaaggcacca caccgtaagc cattccttcg
      361 ctgagcactt taggccagcc ctcggctgcc gacggcagga gaaaaaaatg tgctctggca
      421 taatatgtcg aaatctctgc acggcgtagc catccaataa aatgaatatc atgttgaagg
      481 ttgcagtcag ccaccatagc ctgcagcgtt tctttttcag gcccatcgcc caccaggttg
      541 agctcgaatc ggatgttgtg agttttcaac atgcaggcga tcttcaagat tcgatccagc
      601 cctttcgcct tatccatacg accgacaaat aaaagttgca aaggctgttc cagttgcttg
      661 tttctaccga gaagagaggc ctgcaatact tcctcatcag taaatgaggg gttataaaac
      721 gagaagatat gtggtttttg acttggccag cgaccattca cgctgacaac accagagtgc
      781 agattatttc taagcaacca tcgttgaaat gagtaggacc atgaacctgt ccccaaagca
      841 gcccaattgc ctgcaaattt tacccagcga tggggcactc tattattgat tgccatcctc
      901 tggctggcaa tcagactgat attggaagga cagcgcacat gtacaatatc gttttggcca
      961 aattcactca gcgtttttcc tatcgcccgc aggtattcgg gggtcgccct gataacatcc
     1021 atttttgccc gaaatgaatc tccccctgca ggtcgaacag gtacaaattt tacattcgaa
     1081 gattgataag gcagactgct tgacggcgtg tcatttttat aaaggactgc aaggtgaacc
     1141 acctggtcaa acatgcgtgc tagataatcg atttcacgaa cagttggccc ccatccgaca
     1201 atatgtccat cctgaatata atgcggggtg tgcgagatga cgagcagctt caagaccgtc
     1261 ttcctccaga atcaaggaga agctggcgaa aagctttatt ataacgatcc atgcttactt
     1321 cccaggtttg ctcggtgatc gcgcgctgac gagcctcgag cgacattttt ttgcgtaatt
     1381 cggaattttc cattagagca agaattgtct gcgcggtagt atggggatca ttttcgggga
     1441 ccacgaaccc ggtaacacca tcagacacag tttcggttaa acctgagttt tcagcgacaa
     1501 cggatggctt accgcatagc gcagcttcag aaacgacaat gccataacct tcatactcgc
     1561 catcgcggct atgcctgctc actagcacaa atacatcgca ggcattataa gcagcaaata
     1621 gctcttcttg ttgggctcgc cccataaaat gtacatggtt accaatcccc agtgcattga
     1681 ccagtttctc aagttctggt tgcatggtgg gtagcccggc gatgaggtag tatgtcctcg
     1741 gatttctttc cagaacctcg ggtaacgacc taattacaat atcttggccc tttcgatggg
     1801 acacatgccc aacagtcaat aatacaaaag catctgcgaa tcccatttgc gtgcggagtg
     1861 tttgtccaga tgaaacatcg gggaaaaaga ctttatcatt tgctccgttg ggaatgactg
     1921 ccacgcggga agattgaacg cccagtgata gcaggtgact tttactgtac ctgctgacgg
     1981 caatggtcaa atccggttta ctataagccc acttcatcat cagtttttgg aatggattgc
     2041 tgggatgaat cccatgccag atcgcacacc agggtaattt tatgccagtc ctgtccagaa
     2101 gcccgcgcac tgcttgtgca gcagccgtga gccaaactaa tcggtctccg gttacaacca
     2161 gcagatcagg tttccatttg atcacagttt gctgcaattg ctcccatcga aaacgtaaat
     2221 cggcggcctg gtgcttctgg cgctgtagct gaatgatttc aaaaggctga gtgtgattaa
     2281 attcttctat ttcgctgtgg ctcatataat cctggtatgc cagcacaagc acacgccagt
     2341 gtgacatgct catttgcaac gccatttgat aggcatgtgt gcctatccca cctggtccc
//
LOCUS       DBNHOH010000049         1309 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1155752, whole genome shotgun sequence.
ACCESSION   DBNHOH010000049 DBNHOH010000000
VERSION     DBNHOH010000049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1155752"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="AC16V7_00815"
     CDS             complement(<1..376)
                     /locus_tag="AC16V7_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255687.1"
                     /translation="MKLVVRVLTACLLLALTSLSCNLNGSFEQEEPALASPTPVQAEL
                     ATNTPEASPTTAKTQAPAQPTQTREPTTLPPTNTPTQAVVQIGPCEDEVCLVPFQFPF
                     SRPISPPGRDFVDISYRFGSSDH"
     gene            complement(373..>1309)
                     /locus_tag="AC16V7_00820"
     CDS             complement(373..>1309)
                     /locus_tag="AC16V7_00820"
                     /inference="COORDINATES: protein motif:HMM:NF016825.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HIR6255688.1"
                     /translation="KIAKAVRFSSGGFRFVKALGLLVEGRVQVSMNLTNYRETPLARV
                     VEFIRREAERYGVGIHQSELVGLIPEDALLDAATWYLQLDQFEPDQVLERRLQAALAS
                     SERLTFQFLDALAGDTPTPGGGSASAYSGAAAAALVAMVARLTTGRKKYAAVEAQMQA
                     ALERAEALRRELTAAIDEDAAAFEAVMAAYKLPKDTPQADEQRADAIQSALLGASRVP
                     LEVARKAVEVTRLAALVVEQGNLNAISDGATAAAQARAALTGAGYNVRINAASLQDQD
                     EVRQMQEALNDLEVQAAAHEQAIRQALIQRGGMLF"
BASE COUNT          226 a          394 c          416 g          273 t
ORIGIN      
        1 cgtggtcgct gctgccaaag cgataggaga tgtcgacaaa atctcgaccg ggtggggaaa
       61 ttggccggct aaaggggaac tggaagggca ccaggcacac ttcgtcctcg cacggcccaa
      121 tctggacaac cgcttgggtt ggggtattgg tagggggcaa tgtggtgggc tcgcgcgtct
      181 gtgtgggttg cgcgggcgct tgtgtcttgg cggtcgtagg gctggcttca ggcgtatttg
      241 tggcgagctc tgcctgcacc ggagtaggag aggccagtgc tggttcttcc tgctcaaacg
      301 agccgttcag gttgcagctc aggctggtga gcgccagtaa gagacaggcg gtcaataccc
      361 ggacaaccag cttcaaaaca gcatgcctcc tcgctggata agcgcctggc ggatggcttg
      421 ctcatgtgcg gctgcctgga cttccaggtc attcaatgct tcctgcattt gcctgacttc
      481 atcctggtct tgcaggctgg cggcattgat gcgcacgttg tagccagctc cggtcagggc
      541 ggcgcgtgcc tgagcagcgg cggtggcgcc atcgctgatg gcgttcaggt tgccttgttc
      601 cactaccagg gctgccagtc tcgttacctc caccgctttg cgggcaacct ccagtggcac
      661 ccggctggct cccagcaagg cggattggat ggcatccgcg cgctgctcat ccgcttgcgg
      721 cgtatctttg ggcagcttgt aggctgccat cacggcctcg aaggcggctg catcttcatc
      781 gatcgccgca gtcaattccc ggcgcagtgc ctcggctcgc tccagtgcgg cctgcatctg
      841 cgcctcgaca gcggcgtact tctttctgcc ggtggtcagg cgggcaacca tagcgaccag
      901 ggcggcagcc gccgcaccac tgtaggctga cgctgagccg cccccaggcg tgggggtatc
      961 ccctgccaaa gcgtctagga actggaaggt caaccgctca ctgcttgcca atgcggcttg
     1021 taagcgtcgc tctaatacct ggtctggctc gaattggtcg agctgcaggt accaggtagc
     1081 cgcgtctaac aaagcatctt cagggattaa tccaaccagc tcgctttggt ggatgcccac
     1141 gccatagcgc tctgcctcac ggcggatgaa ctcgaccacg cgagccaggg gcgtctcgcg
     1201 gtagttagtc aggttcatcg agacttggac gcggccttct accagcagcc caagtgcctt
     1261 gacgaagcgg aaaccgcccg acgaaaaacg caccgccttg gcgatcttc
//
LOCUS       DBNHOH010000050         2412 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_579039, whole genome shotgun sequence.
ACCESSION   DBNHOH010000050 DBNHOH010000000
VERSION     DBNHOH010000050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_579039"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1042)
                     /locus_tag="AC16V7_00825"
     CDS             complement(146..1042)
                     /locus_tag="AC16V7_00825"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR6255689.1"
                     /translation="MKKQLAFVLGGGGARGALQVGALRALMEAGYQPDLLVGTSAGAI
                     NATFMAVRGMSLQSITELVQAWHDAATAEFFPQNYLWLTVRALFRRPDVHTSHRLRDF
                     FVSHGLGPELRFGDIQGIRLVLVATDLNASQLALYGLDPAEYVLEGLLASTALPPWVT
                     PIERDGSLLMDGGAISTLPIEPAMLTGATEIIALDLYDPNSLPHEPNGFGPFLAKLLN
                     TVERRQTEMELALASARRVPVHYLHLRAPDSVPLWDFSHAGELIEIGYELARAEIPRW
                     QAESKPRFLKWLQGFIEGPTGP"
     gene            complement(1070..>2412)
                     /locus_tag="AC16V7_00830"
     CDS             complement(1070..>2412)
                     /locus_tag="AC16V7_00830"
                     /EC_number="3.4.22.70"
                     /inference="COORDINATES: protein motif:HMM:NF016119.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HIR6255690.1"
                     /translation="QVKLTASDREEDDSFGSAVAIHKNTVAVAAETKTFNTGSVYVFF
                     RSGVKDWKQQAKLATFPPSFNFGSSIDIYGDLLAVGSTQFNPLEDSGTGGVFLYRREG
                     KVWEKETELEAEKGRIGDAFGNSVAIYGNTVVVGAHHADPDLGAGRVTSAGTAYVFTR
                     SAGAWKEEATLLASNSGAFDQFGQSVDIYGSTIVVGADGATQAGNSGAGAVYVFTKKN
                     GTWNLATRAVTDPTSDDDAFGKSVGVYRDWFIIGASGRDPNSKTRAGEAFLSLLGSVQ
                     LPNTGFAPGQQTILPEQPSSLAYEDYGEMWLEIPSLQQKMTIQGVPKGDNGWDVRWLS
                     RQAGYLEGTAFPTWSGNTGLAGHTVLPDGTPGPFAKLHTLSWGDQVYIHAWGKRYIYE
                     VRENSMVQPNTLKVLKHEELDWVTLITCQGYDEKSGKYRWRRVVRAVLVAVVEE"
BASE COUNT          489 a          711 c          665 g          547 t
ORIGIN      
        1 atgtggcttg aggtcgcttg ctttgtaaaa tctcaactgc ggcagggtgc ccttattcgc
       61 tgggtgtgcg ttgaggctgt tggctgccat caggggcagg gtattaccgt ggagtcgagt
      121 actgccttca cggttcactc ctagtttatg gacctgttgg tccttcaata aatccttgca
      181 gccattttaa gaatcttggc ttgctttccg cttgccagcg gggtatctca gctcgcgcaa
      241 gctcgtatcc gatctcaatt aactcgccgg catggctgaa atcccacaag ggaaccgagt
      301 ccggggcgcg cagatgcagg taatgcaccg gcacgcggcg tgcagaagcc agggctagct
      361 ccatctctgt ttggcgccgt tccaccgtat tcaatagttt tgccaggaaa gggccaaagc
      421 catttggctc atgcggaagg ctgttggggt cgtatagatc gagggcgatg atttcagtgg
      481 ctccagtgag cattgccggc tcgataggca gggtgctgat tgccccgccg tccatcagga
      541 gactgccgtc tctctcaata ggtgtcaccc aggggggcag ggcagtcgaa gccagcaggc
      601 cttccagcac atattccgct gggtccaggc catacagggc tagctgtgag gcattcaagt
      661 cggtagctac caggaccagg cgaatcccct ggatgtcgcc aaagcgcagt tccgggccta
      721 atccgtggga tacaaaaaaa tccctcaggc gatgacttgt gtggacatcg ggccggcgga
      781 aaagggcgcg caccgtaagc cacaggtaat tctggggaaa aaattcagct gtggcagcat
      841 cgtgccaggc ctggaccagc tcggtgatgc tttgcaggga catgccacgt actgccataa
      901 acgtggcatt gatcgccccc gcggaggtac cgaccagtaa atccggttgg taaccggctt
      961 ccatcagggc gcgcagtgcc ccaacctgta gcgctccgcg cgctccaccc ccaccaagaa
     1021 caaaagccag ctgttttttc acatcctcgt ctcatcagct cgttggggcc tattcctcta
     1081 cgacagccac cagcactgcc cggaccaccc ggcgccagcg gtacttgcca cttttctcat
     1141 cgtatccctg gcatgtgatc agcgttaccc aatctagctc ttcatgtttg agcactttaa
     1201 gtgtgttcgg ctgcaccatg gagttctccc gcacctcgta aatatagcgc ttgccccagg
     1261 cgtggatgta aacttgatca ccccaactca atgtatgcag cttggcaaat ggccctgggg
     1321 ttccatccgg caagacggtg tgacctgcca aaccggtgtt gccagaccat gttgggaagg
     1381 cggtgccttc caaataacct gcctgccgtg agagccaacg cacatcccag ccgttgtcgc
     1441 ctttgggcac gccctggatg gtcattttct gctgcaggga gggtatctct aaccacatct
     1501 cgccgtaatc ttcataagcc agggatgatg gctgctcagg caggatagtc tgctgtccag
     1561 gtgcaaaccc ggtattgggg agctgaactg agcccagaag gcttaggaag gcttcgccgg
     1621 cgcgtgtctt ggaatttgga tctctgccgc tggcgccgat gatgaaccaa tcgcgataca
     1681 ccccaaccga tttgccaaag gcgtcatcat ccgaggtggg gtcggtcact gcccgagtcg
     1741 ccaggttcca ggtgccgttc ttcttggtga acacgtacac tgctcctgca cctgagttgc
     1801 ctgcttgggt agccccatct gctcccacca cgatggtgct gccatagata tcaaccgact
     1861 gtccaaactg gtcgaaggcg ccactattgc tcgccaatag cgtggcctcc tctttccagg
     1921 cgcccgcact gcgggtgaat acgtaagctg tgccagcgct ggtgacccgc cccgcgccca
     1981 ggtctgggtc agcgtggtgc gcgcccacaa cgactgtatt gccgtatata gccaccgagt
     2041 tgccaaaggc atcaccaatg cggccttttt ctgcctccag ctctgtttcc ttctcccaga
     2101 ctttcccttc ccgccggtaa aggaagaccc cgcctgtgcc gctatcctcc agagggttga
     2161 actgggtcga gccaacggct agcaaatctc cgtagatgtc tatggaagaa ccaaaattga
     2221 aactcggtgg gaatgttgcc agcttggcct gttgtttcca gtccttgacg ccgctcctga
     2281 agaaaacata caccgagccg gtattaaatg tcttggtttc agcggcgacc gcgacagtgt
     2341 tcttatggat cgccacagcc gagccaaaag agtcatcctc ttcacgatcc gaggcggtta
     2401 gcttgacctg ct
//
LOCUS       DBNHOH010000051         2146 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_515030, whole genome shotgun sequence.
ACCESSION   DBNHOH010000051 DBNHOH010000000
VERSION     DBNHOH010000051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_515030"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..126)
                     /locus_tag="AC16V7_00835"
     CDS             complement(<1..126)
                     /locus_tag="AC16V7_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR6255691.1"
                     /translation="MSKQKFERTKPHMNVGTIGHIDHGKTTLTAAITKYSQILGQG"
     gene            complement(184..256)
                     /locus_tag="AC16V7_00840"
     tRNA            complement(184..256)
                     /locus_tag="AC16V7_00840"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(221..223),aa:Thr,seq:ggt)
     gene            complement(270..352)
                     /locus_tag="AC16V7_00845"
     tRNA            complement(270..352)
                     /locus_tag="AC16V7_00845"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(316..318),aa:Tyr,seq:gta)
     gene            complement(430..505)
                     /locus_tag="AC16V7_00850"
     tRNA            complement(430..505)
                     /locus_tag="AC16V7_00850"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(470..472),aa:Thr,seq:tgt)
     gene            complement(646..1251)
                     /gene="recR"
                     /locus_tag="AC16V7_00855"
     CDS             complement(646..1251)
                     /gene="recR"
                     /locus_tag="AC16V7_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012772148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR6255692.1"
                     /translation="MLLPEPVQKLIDALSRLPGIGPKTASRLAFFLLRAPDDLSLELA
                     QSLQELKTSIVYCQRCFNITTSGRLECEICASPERDGRLLCVVEEPLDVLALERTSGF
                     QGRYHVLGGVLSPIQGIGPDDLKIRELVGRARAEEIKEIILATNPSIEGDATAMYLHQ
                     QLEPLGVRVTRLARGLPVGGDLEYADPNTLLRALAGRQELS"
     gene            complement(1335..1670)
                     /locus_tag="AC16V7_00860"
     CDS             complement(1335..1670)
                     /locus_tag="AC16V7_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIR6255693.1"
                     /translation="MAKGFKGKSPKGSTGSMGMMQQLQKLQEQMEIAQAQLAEETVTV
                     TAGGGAVSITMTGNQNCRAVTIDPELLKDVDVEMLQDLILAAFNGALEKSRQMAADRL
                     GPLASGLPF"
     gene            complement(1836..>2146)
                     /locus_tag="AC16V7_00865"
     CDS             complement(1836..>2146)
                     /locus_tag="AC16V7_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255694.1"
                     /translation="QILALIRKSNTTTEALLRSGRLLGVKDGTVYLGFSEVLKSKMEK
                     RDNMELVTSAFQQVLNMDVPIQCVVSAGKAGSLPPDIESDGMVAAALRDLGGEIVDVK
                     "
BASE COUNT          449 a          595 c          582 g          520 t
ORIGIN      
        1 tccctgtcct aagatctgag agtatttggt gatcgccgcc gtcagcgtgg tcttgccgtg
       61 atcgatgtgc ccaatcgtcc ctacgttcat gtgcggcttc gtgcgctcaa atttctgctt
      121 ggacatattc ttctttctcc ttaaaaagat aaccaaattc accaaagtga cttgggcaac
      181 gagagccctc aatgggattt gaacccatga ccccgttctt accaagaacg tgctctaccg
      241 actgagctat gagggcaatc gccaaaaggt gggcggtgaa ggattcgaac ctccgaaggc
      301 ttctgccaac tgatttacag tcagtccccg ttggccactt gggtaaccgc ccgttcatta
      361 ttgattttag attgtagatt gctgatattg ctatataagc gaaaatcaac aatctacaat
      421 cggctatcct ggagccgacg acgggaatcg aacccgtaac ctaccactta caaggcggtt
      481 gctctgccgc ttgagctacg tcggcgcgca agacgatcgc cagtgttaaa gcgaatggca
      541 ttataccaga ggcataagcc ttgggcaagg tttaggtatt cgattgtcgc tggtgtatgc
      601 agcggggagg gagtctatca ggaattgaga taatcgtcaa tggtcttagc ttaattcctg
      661 ccggcctgcc aaggcgcgca gcagggtgtt cgggtcggcg tattccagat cgccgcccac
      721 cggcaggcca cgcgccaggc gtgtgacgcg cacgccgagc ggctccagct gctggtgcag
      781 gtacatggcg gtggcatcgc cttcgatact gggattggtc gccaggatga tttctttaat
      841 ctcctctgcc ctggctcgtc cgaccaactc gcgaattttc agatcgtcgg gtccgatacc
      901 ctggatgggc gagagcacgc cgcccaagac gtggtaacgc ccctggaatc ccgaagtgcg
      961 ctccagggcc agcacatcca gcggctcctc caccacgcac agcaggcgac cgtctcgctc
     1021 tgggctggca cagatctcgc actcgaggcg cccggaggtt gtaatattga agcagcgctg
     1081 gcaatagaca atgctggttt ttaactcctg tagggattgt gccaactcca ggctcagatc
     1141 gtcgggggcg cgcaacagga aaaatgccag gcgggaggcc gtcttgggac caatgcccgg
     1201 caaacgggac agggcatcga tcaacttctg gacgggttca gggagtagca ttgcagggaa
     1261 gaggtttcaa ctcgaagggc accaaggatc acaaagtggt tatctgtatg ctcgtgtcct
     1321 ttgtgtttat tatcttagaa ggggagtcca gacgccaaag gccccaggcg gtctgcggcc
     1381 atctggcgcg atttctccag cgcgccgttg aaggcggcca ggataaggtc ttgcagcatc
     1441 tcgacatcaa cgtctttgag cagttccgga tcgattgtca ccgctcgaca gttctgattt
     1501 ccggtcatgg tgatcgatac cgcgcctcca ccagcggtga cggtgacggt ctcctccgcc
     1561 agttgcgctt gggcgatctc catctgctct tgcagctttt gcagttgctg catcattccc
     1621 atggatcccg tgctaccctt tggggatttt cctttaaagc ctttagccat ttttcctcca
     1681 atcaaatctc ttcggttctt tgggaatctc agcctcggag tgagcttgtg cggcctattt
     1741 tggtctcgac taccgtaatt cccaaagcat tatatcttcc cgcgggttgc tttgtaagcc
     1801 tgggtattta aataaaacct ttgggaagat gatacttact ttacatccac aatctcgccg
     1861 cccaggtcgc gcaaggcagc cgctaccatg ccatcgctct cgatatcggg cggcaggctg
     1921 ccggctttac ctgccgatac gacgcattgg atgggcacat ccatgttgag cacctgctga
     1981 aaggcgctcg tcaccagctc catattgtcc cttttctcca tcttggattt gagcacctcg
     2041 gaaaacccca ggtagacggt gccgtctttc acgcccagaa gcctgccgga gcgaagtaac
     2101 gcttccgtgg tcgtgttcga cttgcggata agggcaagaa tctggc
//
LOCUS       DBNHOH010000052         3964 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_963627, whole genome shotgun sequence.
ACCESSION   DBNHOH010000052 DBNHOH010000000
VERSION     DBNHOH010000052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_963627"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="AC16V7_00870"
     CDS             <1..976
                     /locus_tag="AC16V7_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR6255695.1"
                     /translation="DRPRPEIQPQTEEKPGWGAYIPAHPSMPSDGRVNGKEMPHVDRT
                     PKMYIGGKQVCPDGHYTRAVLGRKGEIVGQVGDGNRKDIRDAVEAAHAAHTASPGWAM
                     RHGYNRAQILYFIGENLDARRDEFAEQIRAMTGRSLKSAQAEVERAVERLFTYAAWAD
                     KYGGAVQETTLRGVTVAINEPIGVIGIVCPDEYPLLAFVSLMAPAVARGNTVVVIPSQ
                     KYPLSATDFYQVLDTSDVPAGVVNIVTGDRDHLTKTLIEHEDVDSVWYFGSTSGSYHT
                     EYESAANMKRTWVSYGMQRDWLSHEQGEGHEFLHEAIQVKNIWIPTGE"
     gene            complement(1108..1647)
                     /locus_tag="AC16V7_00875"
     CDS             complement(1108..1647)
                     /locus_tag="AC16V7_00875"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6255696.1"
                     /translation="MINSGFPQSFPVIETERLLLREITYDDTSAIFKNFSDPEIAKWF
                     FEQPLTEIEQTKEFIDQFISDFKQGKGLTWAITLKENGQCIGTCGYGDVEIGDRGEIG
                     FDLAQEHWGKGLMSEGLVAVINYGFSVLKLPRVEAHTYSQNERAKHLLEKLGFQLDHV
                     SEDSHYYLLSKDRKKDPGD"
     gene            complement(1685..2920)
                     /locus_tag="AC16V7_00880"
     CDS             complement(1685..2920)
                     /locus_tag="AC16V7_00880"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6255697.1"
                     /translation="MKIFQADRRLVTVLSIVFVQMAGAAMILPILPLFAERHFNMQPT
                     TVTLLVTSYFLAQFFAGPYMGQLSDRYGRIPLLVISQLGSAISFFMMAAAGGPAMLFA
                     ARVVDGITGGNIIIAQAYITDITPREKRTEALGYIMAVFGIGFIIGPALGGLLSAWLG
                     ARMPFVIAGLAAAATALMSRLFLDETVGQKTKNIREFRRVHLTPAEVARNYPLVLILV
                     VAFVGQFGFGMLQSTFALYGSAVLFRGESSQATNLGVGLLLAVVGSGQFITQTWLIGS
                     LKRRFGDPLIVIQGSLIRMTGLAILALVSSPWLAAVSCLFFAVGMGLMMPPLQSLTTR
                     TVSEADRGGVLGLFQSSASLAIILSTAIAGAIFTINPTLPYWIGAILSLAVVLPAWIV
                     LKLSGEGRLKPMEATNPGD"
     gene            3661..>3964
                     /locus_tag="AC16V7_00885"
     CDS             3661..>3964
                     /locus_tag="AC16V7_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HIR6255698.1"
                     /translation="MSKLSQRKTSSQAGSQIEVRPFDGLSRIQPHAAGMDIGAHEIVV
                     CVPGPDSTQIVRGFGTYTAELHDIAQWLLENGIQSVAMESTGVYWIPLFETLESHGF"
BASE COUNT          985 a         1080 c          998 g          901 t
ORIGIN      
        1 ggatcgtccc cgtcccgaga tccaacccca gaccgaggag aagccaggtt ggggggcata
       61 tatacctgcc catcccagca tgccctccga tggcagggtg aatggaaaag agatgcccca
      121 tgttgatcga acgcctaaga tgtatattgg tggcaagcag gtgtgcccag atgggcacta
      181 cacgcgtgcc gtgctaggcc ggaaaggcga aatcgtcggg caggtgggtg acggcaaccg
      241 caaggatatc cgcgatgcgg tggaggcggc tcatgcagcg cataccgcca gcccaggctg
      301 ggcgatgcgt cacgggtata accgcgccca aatcctttac ttcatcgggg agaacttaga
      361 tgcccgccgt gatgagtttg ccgagcagat tcgggccatg actgggcgct cgttgaagtc
      421 agcccaggct gaggtcgaaa gggcggtgga gcggctgttc acttacgctg catgggcaga
      481 taagtacggt ggtgccgtgc aagaaaccac cttgcgggga gtcaccgtcg ccatcaacga
      541 gccgatcggc gtgatcggca ttgtgtgccc agatgaatac ccgctactgg cctttgtctc
      601 gctcatggct cccgcggttg cccgtgggaa taccgtggtc gtcatcccca gccagaaata
      661 cccgctcagc gctaccgact tctaccaggt gctggacacc tctgatgtac cagctggcgt
      721 tgtcaacatc gtcaccggcg atcgagatca ccttaccaaa acccttatcg agcatgaaga
      781 tgtcgattcg gtctggtatt ttggctcaac cagtgggagt tatcacaccg agtacgaatc
      841 ggcagctaat atgaagcgca cctgggttag ttacgggatg caacgcgatt ggctcagcca
      901 tgaacagggt gaaggtcacg agttcctgca cgaagcgata caggtgaaaa atatctggat
      961 tcctactggc gagtgagctc tggcaatagc agactattaa acacaaggcg tttgtaatat
     1021 cctggcaatt gcagataatc ccttgcagtc gtcgctaccc tcgacgactg caagtttatg
     1081 tccaggctat taaatacata aagcgcttca atcgcctgga tctttcttcc tgtctttgga
     1141 tagcagataa tagtgactat cctccgagac atgatccaat tgaaagccta atttttccag
     1201 gagatgtttg gcgcgctcat tttgagagta ggtatgcgcc tctactcttg gtaacttcaa
     1261 tacagaaaac ccgtaattga taacggcgac aagaccttcg ctcattaatc cttttcccca
     1321 atgctcttgc gccagatcaa accctatctc ccctcggtca ccgatttcta catccccata
     1381 gccgcatgtc ccaatacatt gaccgttctc tttcaatgtg attgcccagg tgagtccttt
     1441 gccctgcttg aaatctgaga tgaattgatc gataaattct ttcgtttgct caatttccgt
     1501 cagaggttgt tcgaaaaacc acttggcaat ttctggatcg gaaaagttct tgaatattgc
     1561 gctcgtatcg tcataggtta tttctctaag gagtaaccgt tcggtttcta tgaccggaaa
     1621 gctctgaggg aatccgctat tgatcatttc cttattctcc tttgacccgc caaactaccc
     1681 cgcactaatc tcctgggttg gtggcttcca tgggcttcaa tctgccttcc cctgagagtt
     1741 tcagcacaat ccatgctggg agcacaaccg ccagggaaag gatggcgccg atccaatagg
     1801 gcagggtggg attgatagtg aaaatcgctc ccgcgatggc tgtgctcaag atgatcgcca
     1861 ggctagccga agattggaat aagcctagca ctccgccgcg gtcggcttcg ctcacggtgc
     1921 gcgtggttag ggattgcaaa ggtggcatca tcaaccccat tccaacggca aagaacaagc
     1981 aagagactgc agccagccag ggcgaactga caagggcaag gattgctaaa ccagtcatgc
     2041 gtatcagcga gccctggata actatgagag ggtcaccgaa tctgcgtttt agtgaaccga
     2101 tcagccaggt ttgcgttatg aattgtcccg agcccaccac tgccagcaac aggccaaccc
     2161 cgagatttgt tgcctgactg ctttcgcctc ggaataacac cgctgaccca tacaatgcga
     2221 aagttgactg aagcatacca aaaccaaact gtcccacaaa agccaccacc aggatgagca
     2281 ccaatggata attacgtgcc acttctgcgg gggtcaggtg tacccttcta aattccctaa
     2341 tattcttcgt cttttgaccc acggtttcgt caagaaaaag cctgctcatc aaagcggttg
     2401 ccgccgcagc caaaccagca atcacaaatg gcatccgcgc gcccaaccag gccgaaagca
     2461 agccccctaa agccgggcca atgatgaagc cgataccgaa aacggccatg atgtaaccca
     2521 gggcctctgt gcgcttctcc cgcggcgtga tgtctgtgat gtaagcctgc gcgataatga
     2581 tattcccgcc ggtaattcca tccaccacgc gggccgcgaa aagcattgcg ggcccgccgg
     2641 cagctgccat cataaaaaaa ctgatcgctg acccaagctg gctgatcact aacagaggga
     2701 tgcgaccata ccggtcagat agttgtccca tgtaaggtcc agcgaagaac tgcgccagga
     2761 aatacgaggt gaccagcaaa gtgactgtcg tgggctgcat gttgaaatgg cgctcggcaa
     2821 ataatggcag aataggcaaa atcatcgccg ctcctgccat ctgcacgaag acgatgctca
     2881 atacggtaac taaacgccgg tcggcctgaa atatcttcat gagaacaaaa atcttatgct
     2941 gattagaatc tttgtaagta aaattcagcc catgtggcga tacatggctg aagggtgatt
     3001 gtaaggacgg atggttctgt tgtcaagtga aaatcatcta gcttgtaatt ggcattataa
     3061 atctccccaa cctggtaaat ttaatgatga cattgggcgc aatcagtgag aatgatcacc
     3121 cagctcccac cattcccgtc agtctcttaa acgtcagaat aaactactaa atgatgcaag
     3181 gtacgcatac cttggcccag ccattcgcac gaagcctgcc gctgttccgg tcttgcccgc
     3241 aaacgaatga aaaaccacgg tggaacccaa aaaggtgatg agctcctcag cgaagtctaa
     3301 ccagccgata tgcgcgacac atacacgcct attttttcac tctttccctt taactcgagc
     3361 tgctggtact ccaccccttc tagattcagg ttagctgcaa tcccccgggc tggcggggcc
     3421 agccttcaat ccacaagaaa caggggagca attcgagtat actcccgtcc atgccaggca
     3481 agcaagccga acattcgcat tggtgagaca cctcccgtgc tgcaatctgg tttccggtca
     3541 cgatggtttt ttcactggtg gcgcttcgcc gtgctcagca caggctccga ccccgattag
     3601 caagctatac tttctggtga ctgggcctgg cgtgttagct tctcgaccca ggaggatccc
     3661 atgagcaaac tcagccaacg caagaccagc tcacaagccg gttctcaaat cgaagttcgc
     3721 ccttttgatg gactatctcg tatccaaccc cacgctgccg ggatggacat tggggcgcat
     3781 gaaattgttg tctgtgttcc agggccggac agcactcaaa tcgtgcgtgg ctttggaacc
     3841 tacactgccg aactgcacga tatcgctcaa tggcttctgg agaacggcat tcaaagtgta
     3901 gcgatggaat ccactggggt gtactggatc ccgcttttcg agacacttga atcgcatggg
     3961 ttca
//
LOCUS       DBNHOH010000053         7791 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_258538, whole genome shotgun sequence.
ACCESSION   DBNHOH010000053 DBNHOH010000000
VERSION     DBNHOH010000053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7791)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7791)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7791
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_258538"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..557
                     /gene="nuoI"
                     /locus_tag="AC16V7_00890"
     CDS             54..557
                     /gene="nuoI"
                     /locus_tag="AC16V7_00890"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HIR6255699.1"
                     /translation="MFSEIMRGLSTTFRSMFEKPVTIQYPEVKRPVRKRFKGRHQLHR
                     YENGLEKCIGCSLCAAACPADAIFVESSENSDEERFSPGERYASTYEINYLRCIFCGY
                     CEDACPTEAIVLGDQYELSFTNRRESIYAKEMLLVPLQEGGKPTPQKVEPGMFTRSVP
                     VMKDPMD"
     gene            650..1156
                     /locus_tag="AC16V7_00895"
     CDS             650..1156
                     /locus_tag="AC16V7_00895"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HIR6255700.1"
                     /translation="MMTNELILFLILALVAVVSALGMLFSRNAVYAALFLILNFGTVA
                     VFYLLLNAPFIALTQVTVYAGAIMVLFLFVIMLLGAEQSGRTPAVRWLRPVTISLGAI
                     LLAEAIYIIYTQRSLLPAKLTTSASFGTPNSVGEILFNQYLLPFEVTSVLLLVAMIGA
                     IVLTKPRT"
     gene            1173..1475
                     /gene="nuoK"
                     /locus_tag="AC16V7_00900"
     CDS             1173..1475
                     /gene="nuoK"
                     /locus_tag="AC16V7_00900"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HIR6255701.1"
                     /translation="MLPVAYYIGLSAVLFTIGALGVLIRRNSIVIFMSVELMLNAANL
                     AFVAFARAYESMSGQIFVFFVMTVAAAEVAVGLALIVAIFHSKQSIDVDQMNTMKG"
     gene            1480..3633
                     /locus_tag="AC16V7_00905"
     CDS             1480..3633
                     /locus_tag="AC16V7_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HIR6255702.1"
                     /translation="MLLSEATANAPQFFYLVPWIVFFPVIGLIINMAFGGRLGEKAIG
                     AVACLASGLAFVVSVLLAISLSGHPEGMVVPLAEWITIGELDLQWAFRVDTLSVTMML
                     VVSGVGTLIHIYAVGYMHEDVRHNGDPGRFRRFFVYFNLFIAAMMVLVSGDNYLMLFV
                     GWEGVGLCSYLLIGFWYEKGKDGIGNAIAGKKAFVTNRVGDFGFLIAAFIMFATFRTF
                     DFEAVFAKAEAVAEATPWVITAITLFMLLGVAGKSAQLPLYVWLPDAMAGPTPVSALI
                     HAATMVTAGVYLVARSHPLYVLVPDAQNVVAWVGAATALFAATIAVGQFDIKRVLAYS
                     TISQLGFMVAAVGMGAFVAGMFHLVTHAFFKALLFLSSGSVIQGIERGHHHVEHDPRL
                     KKQLKGHGHDFDPQDMRNMGGLRSRMKTTYWVYLIGALALAGIVPLAGFWSKDEILAE
                     ANHLNSGVYWLLTIAAFFTAFYMGRQILMVFFGEPRSPAAEHAEESPPLMTIPLIILA
                     VLSVLGGALNLPAVHTFGNWLEHTIHLAEAGGEAHTAEAAAAGGFNLTVALISTLLAL
                     AAIALSWWLYDRRTRELQALPAGKRPDDPLRALIGPIFTGMENKWWVDELYWAIILNP
                     YISLSRFLADVIDWRFWHDWFHDVVITGGYNGLSRLLSVRIDLGGIDAFANGLATATQ
                     NLAAQMRRTQTGYVRNYALSVFIGAILILGYLILR"
     gene            3724..5241
                     /locus_tag="AC16V7_00910"
     CDS             3724..5241
                     /locus_tag="AC16V7_00910"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HIR6255703.1"
                     /translation="MNYLLHPLTLVTFFPLLGAVVILFLKAEQKNAARWVALVSSTIT
                     FGISIAMLAQFNSGNPGLQMVINLPWIQVAGWNIAFHMGVDGLSILLVLLTTFLTPIS
                     ILSTWTAIEERVKDFMVFFLLLEVGMVGVFLAQDLFLFYVFWEFTLVPMYFLIGVWGG
                     PRRMYAAVKFFLYTMAGSILMLLAILWLGIQGGSFSVPELIARGGIPANIQIWLFLAF
                     AAAFAIKVPMWPLHSWLPDAHVEAPTAGSVILAGVLLKMGTNGFLRFNLPLFPAAAVQ
                     LAPWMALLAVIGILYGAAVSYAQQDVKKLVAYSSVSHLGFVMLGLFALNPQGIQGGIL
                     QMINHGLSTGALFIIVGMIYERRHTRDMDAFGGLWKVMPIYGAMTLVVTLSSMGLPGL
                     NGFVGEFTILLGAWDAGQSGSLNSVLYAGLAAAGVILAAVYMLFMFQKMFLGPLDKDE
                     NRSLKDLNWREIATLVPLLVFIFWIGLYPKPFFGLMAPAVDNLVSALQTAALAMP"
     gene            5301..6746
                     /locus_tag="AC16V7_00915"
     CDS             5301..6746
                     /locus_tag="AC16V7_00915"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HIR6255704.1"
                     /translation="MTAFDFLVVLPLIVLVSWATLLILADLFIPEDRKVWTVLLAALG
                     LLVTLVILIVQVKPQTVAFNGMVVVDGFAVFLSALFLGSGLVGIALSYDYLKRLGIDR
                     SEYYILLLFSISGMMLMAIAADLIVVFLALELLSIPLYVLAGFARPRPDSEEAALKYF
                     LLGAFAGGFVVYGVALVFGATGTTSLEQVVAAVKIGSANLTLLGTGAALILIGLGFKV
                     AAVPFHMWTPDVYQGAPSTVTAFMAIGAKAGGFAALMRVFVTAFPSLVADLTPILWAL
                     AALTMILGNVVAIAQGNIKRLLAYSSIAHAGYILMAIVSYGNGKVAPDAVASALFYLL
                     GYGLANFGAWAVVIALETVEQKGLALDDYAGLGRKYPGLAAAMVIFMLSFTGVPPTLG
                     FLGKFYLFRTAIEGGFIGLALIGVLTSLVSAYYYLRVVVIMYMKEGEPQVHSEPWLNL
                     TAGGAAVATVVLSIFAAPIFAWAAQAALRLF"
     gene            6800..7141
                     /locus_tag="AC16V7_00920"
     CDS             6800..7141
                     /locus_tag="AC16V7_00920"
                     /inference="COORDINATES: protein motif:HMM:NF013921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR6255705.1"
                     /translation="MPYRQDIFASGQYYHIYNRGAGKGRLFFNPGNYEFLLKLVARYH
                     RNYGAGVIAYCLMPNHYHFLLRQETEEPLSKFINVLFNAYVQAVNRQQDRSGTLFEGR
                     FRHVWIDREEY"
     gene            complement(7443..>7791)
                     /locus_tag="AC16V7_00925"
     CDS             complement(7443..>7791)
                     /locus_tag="AC16V7_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255706.1"
                     /translation="GAIAFTVVGEDYYPSAGYATIDSNGTVGPIHVAAAGLGPTDGFT
                     SYKAFVGDPPRTRWGDYGAAVTDGESIWIASEYIAQTCDLSTYLFGGPIGSCGATRTA
                     LANWATRISKLEP"
BASE COUNT         1407 a         2210 c         2266 g         1908 t
ORIGIN      
        1 cgcggcgatt gtgttgtact tttcgcggca agcgataggt aggaggtagg tagatgtttt
       61 ctgaaattat gcgtggtctc tcgactacgt ttcgatccat gtttgaaaag ccggtgacca
      121 tccagtatcc ggaggtgaaa cgcccggtgc gcaagcgctt caaaggacgt caccaactgc
      181 accgctacga gaacggtctg gaaaagtgca ttggctgctc gctttgcgca gcagcttgcc
      241 cggcggatgc catcttcgta gaatcatccg agaattctga cgaggagcgc ttctctcccg
      301 gagaacgcta tgccagcacc tatgagatta attacctgcg ctgtatattc tgcggctatt
      361 gcgaagatgc ctgccccacc gaggcgattg tccttggcga ccaatacgag ctctccttca
      421 ccaaccggcg ggaatcgata tatgctaaag agatgctgct ggtgccgctc caggagggcg
      481 gcaaacccac tccgcagaag gtcgagccgg gcatgtttac acgttcggtc ccggtcatga
      541 aagatccgat ggattgatat ttgtaggttg atgattgaga attgctgatt tatccatatc
      601 attagcctgg gcgacaatca gcaatctgca atcagaaacc gaggtggaca tgatgactaa
      661 tgaattgatc ctttttctca tcctggcatt ggtggccgtc gtttcggcat tgggtatgct
      721 cttcagccgc aatgcagtct atgcggccct gtttttaatc ctcaattttg gcaccgtggc
      781 ggtattttat ctgctgctaa acgcgccatt catcgccctg acccaggtga cggtctatgc
      841 tggcgcgatc atggtgctct tcttgtttgt catcatgctc ctgggcgcag agcagtccgg
      901 gcgcacgcca gccgtcaggt ggttacgacc tgtcaccatc tccttggggg cgatcttgct
      961 ggcagaggcc atttatatca tctataccca gcgcagcctg ctgcccgcca agctaaccac
     1021 ttcggcctca ttcggcacgc caaacagtgt cggtgaaatc ctgttcaatc aatacctgtt
     1081 gccctttgag gtgacctcgg tcctgctgtt ggttgccatg atcggtgcca ttgtattaac
     1141 caaaccaaga acgtgaaatt ccggaggatt gaatgctccc tgtcgcttac tacattggcc
     1201 tgtctgccgt attgttcacc atcggcgcgc tgggcgtgct gatccggcgc aattccatcg
     1261 tcatcttcat gtcggtggaa ctgatgttga acgcggccaa cctggctttc gtggcattcg
     1321 cccgtgcata tgaatcgatg agcggccaga tattcgtttt cttcgtcatg accgtggccg
     1381 ctgccgaggt agcagtcggt ctggcgctga ttgtggccat cttccacagc aagcagagca
     1441 tcgatgtaga ccagatgaat acgatgaaag ggtgacgaaa tgttactcag cgaagcaacc
     1501 gcaaatgcgc ctcaattttt ctatctggtc ccgtggatcg tcttctttcc agtcatcggg
     1561 ttaatcatca atatggcctt tggcggtcgc ctgggcgaga aggctatcgg cgcggtagcc
     1621 tgtctggctt cagggctggc ctttgtcgtc tcagtgctgc tggcgatctc cctctccggt
     1681 caccccgagg ggatggtcgt cccgctggca gaatggatca ccattggtga attggacctg
     1741 caatgggcat tccgcgtgga cactctctcg gtcaccatga tgctggtagt gtctggggtg
     1801 ggcacgctga tccacattta cgctgtaggt tacatgcacg aagatgtacg ccacaacggc
     1861 gatccggggc gtttccgccg ctttttcgtc tatttcaacc tgttcattgc agccatgatg
     1921 gtgctagtga gcggggacaa ctacctgatg ttgtttgtcg gctgggaggg tgtgggactg
     1981 tgctcgtacc ttttgatcgg cttctggtac gaaaaaggca aggacggcat cggcaatgcc
     2041 atcgctggta aaaaagcctt cgtcaccaac cgggtgggcg attttggctt cctgattgct
     2101 gcctttatca tgttcgccac cttccgcacc ttcgattttg aagcggtttt tgccaaagcg
     2161 gaggcggtag ccgaagccac tccctgggtc atcactgcca tcaccctgtt catgctgcta
     2221 ggcgtggcgg ggaaatcggc gcaattaccc ctatatgtgt ggttgccaga cgcgatggca
     2281 ggtcccacgc cggtctctgc cttgatccac gccgccacga tggtaactgc tggcgtgtac
     2341 ctggtggctc gctcacatcc gctgtacgtc ttggtgcccg acgcccagaa tgtcgtcgcc
     2401 tgggtgggcg cggcgactgc tttgttcgcc gccacgatcg ccgtggggca gtttgacatc
     2461 aagcgcgtgc tggcatactc gacgatcagc cagctgggct tcatggtcgc cgctgtgggc
     2521 atgggcgcgt tcgtggcggg catgttccac ctggtcacac atgccttctt caaggcgctg
     2581 ctatttctct cgtccggttc ggtcatccag ggcatcgagc gcggtcacca tcacgtggag
     2641 cacgacccac ggctgaagaa gcagctaaag ggtcacggtc acgacttcga tccgcaagac
     2701 atgcgcaata tgggtggcct gcgcagtcgc atgaagacca cctactgggt gtacctgatc
     2761 ggcgcgctgg ccctggcagg cattgtgccc ctggcaggtt tctggtccaa ggacgagatc
     2821 ttggcggaag ccaaccacct caatagcggc gtgtactggc tgctcaccat cgccgccttc
     2881 ttcaccgctt tctatatggg gcggcagatc ttaatggtct tctttggcga accacgctcc
     2941 cccgccgccg agcacgctga ggagagcccc ccgctgatga ccatcccctt gatcatcctg
     3001 gcagtccttt ccgtactggg tggagcgctc aacctgcccg ccgtgcacac cttcgggaat
     3061 tggctggagc acaccatcca cctggctgaa gcgggaggtg aggcacacac cgccgaggca
     3121 gctgccgcag gcggttttaa cctcacggtg gctctgatct ctaccctgct ggcgttggca
     3181 gctattgccc tctcctggtg gctgtatgat cgccgcacgc gggagctgca ggccctgccc
     3241 gcaggcaagc gaccagatga ccccctgcgg gcgctgatcg gcccgatctt caccggcatg
     3301 gagaacaaat ggtgggttga cgagctctac tgggcgatca tcctcaaccc ctacatatcc
     3361 ctgtcgcgct tcctggctga tgtgatcgac tggcgcttct ggcacgactg gttccatgac
     3421 gtcgtcatca cgggcgggta taacggcttg agccgcctgc tctcggtgcg tatcgacctg
     3481 ggcggcatcg acgcctttgc caacggtctg gcgacggcta ctcaaaacct ggctgcgcag
     3541 atgcgccgca cccagactgg ctacgtgcgc aattatgcac tgtccgtatt tattggagca
     3601 attttgatcc ttggttatct aatcttgcgg tgatttttac cccactatcc cttatcctct
     3661 ccccctcgtt acctggggaa gcggatagga gataagggat acttatttgg gcgaggacaa
     3721 cttatgaact atttactaca tcccctgacc ctggtcacat tcttccccct tctgggtgcc
     3781 gtggtgatcc tattcctcaa ggcagagcag aaaaacgctg cccgctgggt ggcgctggtc
     3841 tcatcaacga tcacctttgg catttccatc gccatgctgg cgcagttcaa ttccggcaat
     3901 cccgggttgc agatggtcat taatctgccc tggattcaag tagctggctg gaacatcgcc
     3961 ttccacatgg gcgtggacgg cctgagcatc ctgttggtgt tgctcaccac cttcctcacg
     4021 ccgatttcga tcctgtccac atggacggcg atcgaggagc gagtcaaaga cttcatggtc
     4081 ttcttcctgc tgctcgaggt gggcatggtg ggcgtattcc tggcccagga cctgttcctg
     4141 ttttatgttt tctgggagtt caccctggtg cccatgtact tcttgatcgg ggtatggggc
     4201 ggtccacggc gcatgtacgc ggcagtcaag ttcttcctct ataccatggc aggctccatc
     4261 ctgatgttgc tcgctatcct gtggctgggc attcagggcg gcagcttctc tgtgcctgag
     4321 ctgatcgcca ggggtggcat tcccgccaat atccagatct ggcttttcct ggcttttgcc
     4381 gctgcctttg ccatcaaggt gcccatgtgg ccgctgcatt cctggctgcc cgacgcccac
     4441 gttgaagcgc ccacggctgg ctcggtcatc ctggcaggcg tgctgctaaa gatgggcacc
     4501 aacggtttcc tgcgtttcaa cctgccgctc ttccccgccg cggccgtgca actggcgccc
     4561 tggatggcgc tgctggcggt gatcggcatc ttgtacgggg cggcggtctc ttacgcccag
     4621 caagacgtga agaaactggt ggcgtactcc tcggtcagcc acctgggttt cgtcatgttg
     4681 ggtttatttg cgctcaaccc gcagggcatc cagggtggca tcctgcagat gatcaaccac
     4741 ggcctgagca ccggggcgct gtttattatc gtcggtatga tctacgaacg ccgccacacg
     4801 cgggatatgg acgccttcgg cgggctgtgg aaggtcatgc ccatctacgg cgccatgacc
     4861 ctggtggtga ctctgtcatc tatgggcttg cccggcctga atgggtttgt cggcgagttc
     4921 accatcctgc tgggcgcatg ggacgccggg caaagcggct cgttgaactc ggtgctatat
     4981 gccggcttgg ctgcggctgg cgtcatcctg gcagctgtgt atatgctctt catgttccag
     5041 aagatgttcc tcggcccgct ggacaaagac gagaatcgct ctctcaaaga cctcaactgg
     5101 cgtgagatcg ccaccctggt gccgctgttg gtctttatct tctggattgg cctgtatccc
     5161 aagccgtttt tcgggctgat ggccccggcg gtggataatc tggtcagcgc gctgcagacg
     5221 gctgcgctgg caatgcccta gagatgtgtg tgacgatacc ctgagaggca tattgcactg
     5281 catcaattaa aaggaagtct atgaccgctt tcgattttct cgttgtcttg ccgttgattg
     5341 tgctggtctc gtgggccacg ctgctcatcc tggccgacct gttcatccct gaagaccgca
     5401 aagtctggac cgtcttgctg gcggcgctgg gcctgctggt caccctggtc attttgattg
     5461 tacaggtaaa gccccagacc gtagccttca acggcatggt ggtggtggat ggattcgccg
     5521 tattcctgtc tgctctattt ttgggcagcg gcttggtggg catcgccctg tcttacgact
     5581 accttaagcg gctggggatc gaccgcagtg agtattacat cctgctgctc ttctctatca
     5641 gcggcatgat gctgatggcg atcgccgccg acctgatcgt agtcttcctg gcgctggagc
     5701 tgctctccat cccgctgtat gtgctggcgg gcttcgcccg ccccaggccg gattcggaag
     5761 aggcagccct gaaatacttc ctgctgggcg ccttcgccgg gggttttgtg gtgtatggcg
     5821 tggcgctggt cttcggggcg acgggtacca cctcgctaga gcaagtggtg gctgcggtca
     5881 agatcggctc tgccaacctg accttgctgg gcaccggcgc agccctgatc ttgatcgggc
     5941 tgggcttcaa ggtggcggcg gtgcccttcc acatgtggac gccagatgtg taccagggcg
     6001 cgcccagcac agtgacggct ttcatggcga ttggcgccaa ggctggcggc ttcgccgcgc
     6061 tgatgcgcgt attcgtgacg gcttttccca gtctggtggc tgacctgacc cccatcctgt
     6121 gggcgctggc ggcgctgacc atgatcctgg gcaacgtagt tgccatcgcc cagggcaata
     6181 tcaagcgcct gctggcttac tccagcatcg cccacgccgg ctatatcctg atggccattg
     6241 tctcctatgg gaacggaaag gtcgccccag acgcggtggc ttcagccctc ttttatttgc
     6301 tgggctacgg tttagccaat tttggcgcct gggcggtggt gatcgccctg gaaaccgttg
     6361 agcaaaaagg gctggcgctg gatgattacg ctggcctggg gcgcaaatat ccgggattgg
     6421 cggcggccat ggtcattttc atgctcagct ttaccggcgt gccgcccacc ctgggcttct
     6481 tgggcaagtt ttacctgttc cgcacggcca tcgaaggggg cttcatcggc ctggcgctga
     6541 tcggcgtgct gacctccctg gtctcggcct actactacct gcgggttgta gtgatcatgt
     6601 atatgaagga aggcgagccg caggtgcaca gcgagccgtg gctcaacctg accgccggag
     6661 gggctgccgt ggcgacggtg gtgctgagca tcttcgccgc gccgatattc gcctgggcgg
     6721 cccaggcagc gctgagattg ttctgatcga aaacgtcccg caggtcaaag acctgcggga
     6781 ggtttgatta aaactgctga tgccctatcg acaggatatt ttcgcctcag gtcagtatta
     6841 ccacatctac aatcgcgggg ctgggaaagg gcggcttttc ttcaatcctg gcaattatga
     6901 atttctgctc aaattggttg cgcgttacca tcggaactat ggggctggcg ttatcgccta
     6961 ttgcctgatg ccaaaccatt atcactttct gctacgacaa gaaacggaag agccattatc
     7021 aaaatttatc aatgtgctgt ttaatgccta tgttcaggct gtgaatagac agcaggatcg
     7081 cagcggaacc ctgtttgaag gtcggtttcg gcatgtttgg attgatcgag aagagtatta
     7141 ggtccacctc tgccggtaca ttcactggaa cccggtcaag gcaaacctgg tatctaaccc
     7201 cgaggattgg ccttattcaa attatttgga gtggattggg cgtaggaagg gggtattaaa
     7261 ggatgactct ttcatcagcg agcgcttcca tacacctgag tcctaccaac actttgttct
     7321 aagctatcca gaaaatgatc aagagaacga gcggatcatg aaatttgtgt gggactagta
     7381 acgtcccgca ggttcggaac ctgcgggacg ttttttaaca cacaaactag ctactggacg
     7441 gcttatggtt cgagcttgct gatgcgcgtg gcccagttcg caagtgctgt gcgggtggcg
     7501 ccacaactcc cgatcggtcc accaaacaga tatgtggaga ggtcgcatgt ctgggcgata
     7561 tactccgagg cgatccagat agattcgcca tcggtcacgg ccgcgccgta gtcgccccag
     7621 cgggttctcg gcgggtcgcc gacgaacgct ttgtagctgg tgaacccgtc cgtcggaccg
     7681 agcccagcgg cggcgacgtg gatcgggccg accgtgccat tggagtcgat ggtcgcataa
     7741 ccagcgctgg gatagtagtc ctcgccgacg accgtgaatg cgatggcgcc c
//
LOCUS       DBNHOH010000054         1966 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1027677, whole genome shotgun sequence.
ACCESSION   DBNHOH010000054 DBNHOH010000000
VERSION     DBNHOH010000054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1027677"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="AC16V7_00930"
     CDS             complement(<1..431)
                     /locus_tag="AC16V7_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR6255707.1"
                     /translation="MADQNIRRRDFIKLSAFGAAATAILRLARRVSASDPSGTSPHKW
                     AMVIDQAKCTGCGYCTLACRAHNDVPPDISWNRVIEVGDYGDRKVYLSRPCMHCEHAP
                     CVEVCPVKASYYRPDGIVMMNYDRCIGCRYCEVACPYGGRA"
     gene            complement(434..1345)
                     /locus_tag="AC16V7_00935"
     CDS             complement(434..1345)
                     /locus_tag="AC16V7_00935"
                     /inference="COORDINATES: protein motif:HMM:NF024827.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HIR6255708.1"
                     /translation="MLRFISRLWYGLLFAIPIMLITYALAEAANRPQETPTGELNCRE
                     CHESFVQAWETGSHGQATSDPAFREAWMAAGQSPECLSCHVTGYDEETNTWESDGITC
                     KACHSPITLNHPLAPMSSDRSANMCGDCHTETFFEWQISGHRNAGVSCSACHDPHATD
                     LKTTDKTLVCADCHRNRQSNFAHSQHSQAGLTCGDCHLQKISETGNEGKAKQSHSFIV
                     GLTACNSCHSYQMHDPVEVHPDASTPEPADAMSAVESLSVTPEPIPVSPMGYTTLSGL
                     IGVAFGVILAPYIERISRRNRDDKEEE"
BASE COUNT          523 a          500 c          434 g          509 t
ORIGIN      
        1 aaagccctgc cgccataggg gcaagccact tcacagtacc ggcacccgat acaccgatca
       61 taattcatca tcacgatgcc atcaggtcga taatagctag cttttaccgg gcaaacctcc
      121 acacatggag catgctcaca gtgcatacag ggtctagata agtacacctt tctatcacca
      181 taatcaccca cctcgatcac tcgattccag gagatatctg gaggaacgtc attgtgagcc
      241 cggcatgcca acgtgcaata accgcatccg gtacacttgg cctgatcgat caccatagcc
      301 cacttatgag ggcttgttcc actcggatca gaggccgaaa cccttcgtgc taaccgaagg
      361 atagccgtcg cggcagctcc aaatgccgaa agcttgataa aatcgcgccg cctgatattc
      421 tggtcagcca tgtttactcc tcctccttat cgtccctgtt gcgacggctt atacgttcga
      481 tataaggcgc caggatcacc ccaaatgcca caccgatcaa tcctgataat gtcgtgtacc
      541 ccattggact cactggaatc ggttcagggg ttacggataa ggactcaact gcagacatcg
      601 catcagctgg ttcaggcgtt gatgcatcag ggtgaacctc taccgggtca tgcatttggt
      661 atgagtgaca actgttgcat gcagtcaatc ccacaatgaa gctgtgcgat tgcttggcct
      721 tgccctcatt tcccgtctca gatattttct gtaggtggca atcaccgcag gttaacccag
      781 cctggctgtg ctgagaatgc gcaaaattcg attggcgatt acggtgacaa tcagcgcata
      841 ccagtgtctt gtctgttgtt ttcaaatcag tagcgtgagg gtcgtgacat gcagagcagc
      901 tcacccccgc attacgatgt ccactgattt gccattcaaa aaatgtctcg gtgtgacaat
      961 cgccgcacat attggcggaa cgatccgaag acataggcgc aagaggatga ttcaacgtaa
     1021 tcgggctatg gcaggctttg caagtgatcc catccgattc ccaggtattg gtttcttcat
     1081 cgtaacctgt aacgtgacaa cttaagcact caggagattg gcctgccgcc atccatgctt
     1141 ctcggaaggc tgggtcagat gttgcttgtc catgactacc agtctcccat gcctgcacga
     1201 atgattcgtg acattcccta caattcaact cgccagttgg tgtctcctgg ggtcggttgg
     1261 ctgcctcggc caatgcataa gtaatcagca tgatcggaat ggcgaaaaga agtccatacc
     1321 ataagcgcga aataaatcgt aacatttttg cacctcttat ctatagatga ttatggtgaa
     1381 gctggtattg gtgctcttcg gaagacctta cagagaaggc aaccctgcat cagttgacca
     1441 tgattgtttt gccggtagta ttgccagcaa ggcacttcag gatgtttata tgcatcacag
     1501 gttgcgcgac gttcttggga gcacttatta atatcccaac atccaatatt tcgagctctc
     1561 acttcaatcg ggaccttctc agcttcagtt tgccagtgtt catccaatct tttgagcagg
     1621 atgatcatca acgcggtcaa gccgaatgga ataattattc gaaccacaag ccctatgatc
     1681 atagctatta gcgtggagag ccactccata aagcacctca atttttagaa taaataaaat
     1741 tgtctgcgtc catacctcga accgatgaaa agcaacaatc ctaagggaat aagtattctg
     1801 acgaaaaaca aaatggtgaa tgcaaggatt gtctccataa ctatctccct atccaaactt
     1861 aaaatctatt aatgttttct ctcagtatag gaatagagag ctgagatgta aatccagcag
     1921 actaccaatc tttttatgtg aatttatggt gataattgcc catgat
//
LOCUS       DBNHOH010000055         3779 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_194710, whole genome shotgun sequence.
ACCESSION   DBNHOH010000055 DBNHOH010000000
VERSION     DBNHOH010000055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_194710"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="AC16V7_00940"
     CDS             <1..711
                     /locus_tag="AC16V7_00940"
                     /inference="COORDINATES: protein motif:HMM:NF024872.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6255709.1"
                     /translation="PVPAIQNDDLDTFLELAMTREPDGYALTIIDTVGRAMQGLNENA
                     QEHASAFTALVQKIQYAFDGAVLAIHHTGHQEGERTRGSSVFTADADTIVRAHRKSKT
                     TSAVSLSMVKQKDATEWEQPKIFIARKIEDHDSLAIDTPAPAEQVTATPPVKADSIML
                     PLVERVTLEILQQYPSKTWSERKLAEQVARHEDIDIKYETVRGHLRELKLDKKSKLFE
                     YYDPSLSANAGQWQYKKK"
     gene            complement(1365..1595)
                     /locus_tag="AC16V7_00945"
     CDS             complement(1365..1595)
                     /locus_tag="AC16V7_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255710.1"
                     /translation="MDDFTKTQLIHRLNRAKHARQNGSHVVYGEKSLPPDIPTHLMPA
                     LTLQDPDQLGDALQRELLKSLAQRGRVSKVNG"
     gene            complement(1705..2664)
                     /locus_tag="AC16V7_00950"
     CDS             complement(1705..2664)
                     /locus_tag="AC16V7_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255711.1"
                     /translation="MQRKGLTHDMVAHLWANHATEGGMVEGFNMFADETSIWSYGHHF
                     EIARHVTNEQGERAILITNDSYSVSTAKHIGKVCRAINYGHDTPTFTCELSRDVQPLD
                     VWWDYDAQIAEQMKFSRGAHYDQVNARVSDAQRFAHLNRAQELCDTANKLRNFFALKV
                     KPQSLLKSDREWVEKHTYARRWNGVVADCVNMINEQADTYKWEVGGDLKRWRNGENIH
                     PPQSLRNRIYLRRINRPAIKEDIIQTSWGATFPTEQAIAAWPIIRRCFQRGETFIRNG
                     SQVRLGQFQIDAIKANGDVEAGCHNVSFDEIKRLAEELNLETI"
     gene            3382..3666
                     /locus_tag="AC16V7_00955"
     CDS             3382..3666
                     /locus_tag="AC16V7_00955"
                     /inference="COORDINATES: protein motif:HMM:NF048882.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Acb2/Tad1 domain-containing protein"
                     /protein_id="HIR6255712.1"
                     /translation="MMKDVMREVFTPLTTEQKTHAAEVKHEANRLYALLEEMTAFYPD
                     GAKELAQARINLEQASMWAVKGVTAEVTSRDVKPVMKYMSGSTPDMDNEH"
BASE COUNT          887 a          919 c         1046 g          927 t
ORIGIN      
        1 ccggtgccag cgatccagaa tgacgacctc gatactttcc tagagttagc gatgacgcga
       61 gaacctgacg gctatgcgct gacgatcatc gacacggtcg gcagagcgat gcaggggttg
      121 aacgagaatg cgcaggagca tgcgtcagcg ttcacagctc tcgtgcaaaa aatccaatat
      181 gctttcgatg gcgctgttct ggccatccac catacgggtc accaggaagg cgagcgcacc
      241 cgaggctcct ccgtcttcac cgccgacgca gacaccatcg tcagagcgca caggaaatcg
      301 aagacaacga gcgctgtgtc tctcagcatg gttaagcaaa aggacgcgac ggaatgggag
      361 cagccgaaga ttttcatcgc cagaaaaata gaagaccacg acagcctcgc catcgacacg
      421 ccagcgccag ctgaacaggt tactgcaaca ccgcccgtta aagctgactc gatcatgctc
      481 cccttggtcg agcgcgttac gttggaaatt cttcagcaat acccgagcaa gacatggtcg
      541 gagcgcaagc tggctgagca ggttgcgaga catgaggata tagacatcaa gtacgagacg
      601 gtgcgcggcc atctcaggga attgaagctc gataaaaagt cgaagttgtt cgagtattac
      661 gacccatctt taagtgcaaa tgcaggtcaa tggcagtaca agaagaagta gtgcaatctc
      721 tttgaaatta acttaaggtt aacgtgggtg ggtggaggct ccacccactt tgggtgaagt
      781 gggtgaagtg ggtggtgggt ggcgagaatg gcagaaatca gccacaaaat aggtgggtgg
      841 gtggtgtggg tggttaaagc tgtatattag tgggtggagt attttatata ccccctaaag
      901 gggttataaa atatcacact aatacttttt gacatcacgt gatatcagat ccacccactt
      961 gaaaaagtgt aggttggttt ttgagttgtg ctggttattg gggagctgga gcgggctgga
     1021 agagacgacg acatttttaa gtcgtgctgg ttattgggga gttggaggtc tgctcgcctg
     1081 tggcgagagg tcacgtccgg aagagacgaa gacattttta ggtcgtgctg gtaattgggg
     1141 aaaagatccg cgaacgcgaa gcggtggcct atcgaatgtt ggcgggagct gatcgcatgg
     1201 cgtggtggga ggtggtggta aaaaaaatgc cagcccgtag gctggcaaag tttcacgaag
     1261 gtatgtttta atttattgcc cggtgattat gggcgcgatg gatcctaaca agtagataaa
     1321 gcttgccagg atccagattg caattagagc catctcgagc cagctcatcc attgaccttc
     1381 gacacgcgcc cacgctgtgc tagggatttc agtagctcgc gttgcaacgc gtcgcccagt
     1441 tggtcaggat cctgtagggt gagtgctggc atgagatgcg tgggtatgtc tggtggtagg
     1501 gacttctcac catagaccac gtgcgagccg ttttgccgcg cgtgcttagc acggttcaat
     1561 cggtgtatta attgcgtttt tgtgaagtca tccattgtct tttgtttccg ttggttgggt
     1621 ttgggtaatt gctcaagctt ggagcgccac ccgcggcgag cggtgtaaca actggcctgc
     1681 ggccagcgct ccaagtatca gcaattagat agtttcaagg ttgagttctt cggcgagccg
     1741 tttgatctcg tcgaatgaca cattatggca accggcttcc acgtctccgt ttgccttgat
     1801 cgcatcaatc tggaactgac caagccgaac ttgcgagccg ttgcgaatga aagtttcgcc
     1861 gcgttgaaag catcggcgaa ttattggcca agccgcaatc gcttgttcgg ttggaaatgt
     1921 agcgccccaa gacgtttgga taatatcttc tttgatcgct ggccgattaa tgcggcgcag
     1981 atagatgcga ttgcgcaagc tttgcggggg atggatattt tcaccattgc gccagcgctt
     2041 caagtcgcct ccgacttccc atttatacgt gtcggcttgc tcgtttatca tattcacaca
     2101 atccgcgacc acaccattcc agcgccgcgc gtaagtatgc ttttccaccc attcgcggtc
     2161 tgatttgagt aagctttgcg gcttcacctt gagcgcaaag aagttgcgta atttgttcgc
     2221 ggtgtcgcaa agttcttgcg ctctgtttag gtgcgcaaag cgctgcgcgt cggaaacacg
     2281 cgcattgact tggtcatagt gagcgccgcg cgaaaatttc atttgttcgg cgatttgtgc
     2341 gtcataatcc caccagacat cgagcggttg cacgtcgcgc gatagctcgc aagtaaacgt
     2401 tggcgtatcg tgtccataat taattgcgcg gcaaactttg ccaatgtgtt tggcggttga
     2461 aactgaatag ctatcgttgg tgatgagtat tgctcgctcg ccttgctcgt ttgtgacgtg
     2521 gcgtgcaatc tcgaaatggt ggccatatga ccaaattgaa gtctcatcgg cgaacatgtt
     2581 gaaaccctca accatcccgc cttccgttgc gtggtttgcc caaagatgcg ccaccatgtc
     2641 atgcgtcaaa cctttgcgtt gcattgtctt atctccgtct tgctcagttg ataccagccg
     2701 ctgtggcgat tgtgcgcacg attgtgaagt catcagcaat cgcgtagcga attaggatga
     2761 acgcgcatag cgccaggaaa catgtgccga tggtttgcat gatttataca ctccgtggtt
     2821 taaactcagt ttgagcataa gtaggttagg ggttgttttg gtcttgtttt gagataggct
     2881 catgaagagc ataattagcc acccaggctt atcccacgac gccacatcgc gtcgcgcgga
     2941 tagcctgcca taaatctatt gacgacgtca acctattacg tattgcgagc atttcttgag
     3001 ctttgtgcac gcgatgccgc agctggccgc gccgatgcta ccgcatgctc gcgctaatca
     3061 taggttgtgc catggcgtat tgtgcacgcg ccagcctcag ctcagcatta gtatcatttg
     3121 agagcatcgg cagatcgcag gcagatcggt aggggggtgg ggtcgcgccg aggcggttcg
     3181 ggggcatacc cctcgctctg ccctggtagg ttgaccgtga gacaaacgct tttcccacga
     3241 cgtcccaaaa catcttgacc gaatcaatta ataaaaaaaa aaaccaaatg cccacgattt
     3301 cccaaaacat gttgacccag tcaaacattt acagtagctt cgaccgctct tgtttgcata
     3361 tactcaacac acgaggagaa aatgatgaaa gatgttatgc gagaggtttt caccccccta
     3421 acgaccgagc aaaaaactca tgctgctgag gtcaagcatg aggccaacag gctgtatgcg
     3481 cttctcgaag agatgaccgc attctacccc gacggagcca aggagctggc ccaggcgcgg
     3541 attaacctcg aacaagcctc catgtgggcg gtcaaggggg tgacggctga agtgacatca
     3601 cgtgatgtca agccagtgat gaaatatatg tccggctcta ctccagatat ggacaacgaa
     3661 cactagatat agtatcttgc cgatggcccg acctccttgt ccggttggtc cctccttgct
     3721 ttgcggtcgg ggttccggcg tttttcctct ccgtcggaac cccacacctc ctcgcaagt
//
LOCUS       DBNHOH010000056         3316 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1220258, whole genome shotgun sequence.
ACCESSION   DBNHOH010000056 DBNHOH010000000
VERSION     DBNHOH010000056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1220258"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="AC16V7_00960"
     CDS             <1..743
                     /locus_tag="AC16V7_00960"
                     /inference="COORDINATES: protein motif:HMM:NF013125.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005882 - intermediate filament [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HIR6255713.1"
                     /translation="APGHSIERYPPFQDSNTAQDWRDQPDPSPADVDNTPPTPTPSAT
                     PTSINTPTPTPTATPTQTQTPTPTPTMIPGLVINEVHADPDADFGDANGDLVVDTYDD
                     EFVEIVNATGGAIDLNGWRLNDRVGERHVFPPGSLLPNGCSVVVFGGGNPSGGFGGSL
                     VQTASTGGLSLNNEGDRLTLYNASSTAVAAIGYGAEANDDQSLTRYPDITGPEPMVKH
                     ATIPEAGGKLFSPGKRLDGAVFSGCPAN"
     gene            complement(815..1555)
                     /gene="lexA"
                     /locus_tag="AC16V7_00965"
     CDS             complement(815..1555)
                     /gene="lexA"
                     /locus_tag="AC16V7_00965"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HIR6255714.1"
                     /translation="MMARRRTKGLSERQKRILEVLERFQNQNGYPPSIREICEKTNIS
                     STSVVNYYLNQLEEAGYIERDGRVSRGIRLLRPLTEITGSAAAALKQTAQSVRETVEE
                     LLHIPVVGRIVAGEPVPVPQSDFSYFDADSMVDVARSLLPAREKGGSNLFALEVKGHS
                     MIDAMVNDGDIVIMKQAREARNGEMVAVWLNDRDETTLKYFYLENGTVRLQPANPTMD
                     PIFIDDPSLVEIQGKVVMVIRKVGGLPS"
     gene            1741..2325
                     /locus_tag="AC16V7_00970"
     CDS             1741..2325
                     /locus_tag="AC16V7_00970"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016107.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR6255715.1"
                     /translation="MIILLAVFVYSLFHSILASLWVKARARAWFGLETERWYRLAFNA
                     IAVVSLVPVLTLPALLPDRNVYTIPFPWAYINLAIQGLAGLALIVGLWQTGLWSFLGI
                     QQFLTPPKSDPPKLVVGGLYRWVRHPLYTAGLALIWFIPVMTTNLLALILGLSIYLVV
                     GALFEERRLLVEHGEAYAEYQRRTPMLIPTRFPR"
     gene            2741..3151
                     /locus_tag="AC16V7_00975"
     CDS             2741..3151
                     /locus_tag="AC16V7_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HIR6255716.1"
                     /translation="MTERKIRVLMAKPGLDGHDRGVKVVSRALRDAGMEVVYTGLRYT
                     PEMIAEAALQEDVDIVGLSILSGAHMVLVPRVLELLKANGQEHVKVFVGGIIPNEDVP
                     RLMEMGVVGVYGPGTITDEIIKDVRQAVAGAAVA"
     gene            3158..>3316
                     /locus_tag="AC16V7_00980"
     CDS             3158..>3316
                     /locus_tag="AC16V7_00980"
                     /inference="COORDINATES: protein motif:HMM:NF015279.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HIR6255717.1"
                     /translation="MGVVDSVLEGNRLSLARLLTQIENGVPEGRAAMEALYPHTGKAH
                     LIGVTGAPG"
BASE COUNT          699 a          906 c          915 g          796 t
ORIGIN      
        1 tcgcaccagg tcacagcatc gagcgttacc cgcccttcca ggatagcaac actgcccagg
       61 actggcgcga tcagccagat ccgtcacctg cggatgtgga caacacgccg ccaaccccca
      121 cgccatctgc cacgcctacc tcgatcaaca cgccgacccc cactcccact gcgacgccaa
      181 cccagacaca gacgcccacc cccacaccta ccatgatccc aggcctagtg atcaatgagg
      241 tccatgccga cccggacgct gactttggag acgccaacgg cgatttagtg gtcgatacct
      301 acgatgacga gttcgtcgaa atcgtcaatg ccaccggcgg ggccatcgac ctgaacgggt
      361 ggcgtctaaa tgatcgtgtg ggcgagcggc atgtctttcc cccaggttcg ttgcttccca
      421 acggttgctc tgtggtggtc ttcggcggtg ggaatcccag cgggggtttt ggcggcagcc
      481 tggtgcaaac ggcctcgact ggcggcctct ctttaaacaa cgaaggagac cggttgacct
      541 tatataacgc ctcgtctaca gcggtggcag caattgggta tggcgcggag gccaatgatg
      601 atcaatccct gacgcgctac ccggatatca ccggcccgga acccatggtg aaacatgcca
      661 ccatacctga ggcaggtggg aagctcttct cgcccggcaa acgcctagat ggggctgtat
      721 tctccggctg tcccgcgaat taagcaggaa cgggctgcga ccagcccgtt cctttaccac
      781 tgccttcgct tcctccagag gaggcgagag gattttacga aggcaatcca cccaccttgc
      841 ggatgaccat tactactttg ccctgtatct ccaccaagga gggatcatca ataaatatag
      901 gatccatggt gggatttgca ggttgcaggc gcaccgtacc gttctcgaga tagaagtatt
      961 tcagggtggt ctcatcgcgg tcgttcagcc ataccgccac catctcccca ttgcgtgctt
     1021 cacgggcctg cttcatgatg acgatgtcgc catcattgac catggcgtcg atcatggagt
     1081 ggccctttac ttccagggca aagagattgc tgcccccttt ttcacgggcg ggtagcaggc
     1141 tgcgagcgac gtccaccata ctgtcggcat cgaaatagga gaagtccgac tgtggcaccg
     1201 gaaccggttc gccggctaca atgcgcccta caacagggat gtggagcagc tcttcgaccg
     1261 tctcgcgcac cgattgggcg gtctgcttca aggcggcagc tgcggaaccg gtgatctccg
     1321 tcagcggtcg caacaggcga atccctcgcg agacccggcc atcgcgctca atataaccgg
     1381 cctcttcgag ctggttcagg taatagttga ccaccgatgt ggaagagata ttggtctttt
     1441 cacagatctc gcgaatcgac ggagggtatc cgttctggtt ctgaaaacgt tccagcacct
     1501 ccaggatacg cttttgtcgc tcactcaatc ctttggtacg tcgccttgcc atcatagtct
     1561 tttctcctgt ccaatcttgg caaattaaag gacatccatt aattcgttca tttgtgctaa
     1621 aaatatatta ccagaacatt cgttctgtgt caagacctgt tttttaggaa tttaacggga
     1681 caatttgcat atttgacgaa ttgcgataaa ctattgccaa cctatccgat gagatcattt
     1741 gtgattatcc tcctggctgt ttttgtatac agcttattcc actcgatact ggcctccttg
     1801 tgggttaaag cgcgcgctcg tgcttggttt ggactggaaa ccgagcgttg gtaccggctg
     1861 gcatttaatg ccatcgcagt ggtcagtttg gtgccggtgt taaccttgcc cgctctgttg
     1921 cccgaccgga acgtttacac catccccttc ccatgggcat acatcaactt ggctatccaa
     1981 gggctggcag ggctggcgtt gatcgttggc ttgtggcaga ctggcctatg gtctttcttg
     2041 ggaattcagc aatttctcac ccctcctaaa tctgatccgc caaagctggt ggtgggtggg
     2101 ctgtatcgct gggtgcgcca cccgctctat acggctggat tggccttgat ctggttcatc
     2161 cccgtgatga ccactaatct cctggcgctg atcctgggtc taagcattta cctggtggtg
     2221 ggggcgttgt tcgaggagcg caggttgctg gttgagcacg gtgaggcgta tgctgagtat
     2281 caaaggcgaa caccgatgct gatcccaact cgtttcccta gataaaaaat gctcactata
     2341 ttgcacgcct ttgaaagtga gactccttcc ttttttgtat gttcggaggg cggcagctta
     2401 tattgacaat tcggggcttt catgtatact aatcccattg agcggggctt accgctctct
     2461 cctctgctat cccacccaag aggaggtgat gccgacatgt tagacagtag taccatacga
     2521 agcgctgcgg catccctcct cgcaatagga taggttgccg cagcgctgca aggagccgtt
     2581 cgaaagagcg gcttcttgtc ttaatcggca taggtgtgct tggtttgcaa tacttaggga
     2641 aaccagttct catggaaatg cttgtggcga aaggggagta aagggaatat gcgacgcggt
     2701 ataatcgaat tgccaagcaa tcatcacatg gagaaaatat atgaccgaac gaaagatccg
     2761 cgttctgatg gcaaaacctg gtttagacgg gcatgaccgg ggggtaaaag tcgtttcgcg
     2821 cgccctgcga gatgccggta tggaggtcgt ctacaccggt ctgcgttata cccccgagat
     2881 gatcgcagaa gcggctttac aagaggatgt ggatattgtc gggctttcca tcttgtctgg
     2941 agcgcatatg gtacttgtac cgcgcgtcct ggaactgctc aaagccaatg ggcaggagca
     3001 cgtcaaggtc tttgtcggcg gtatcatccc caatgaggat gtgcctcgtt tgatggagat
     3061 gggcgtggta ggtgtgtatg gccccggtac gataaccgat gagatcatca aggatgttcg
     3121 ccaggcggtg gctggggcgg cggtggctta agctctcatg ggagtggttg actctgtatt
     3181 ggaaggcaac cggctctcgt tagccaggtt gctcacccag attgagaacg gcgtgcccga
     3241 aggtcgcgct gccatggagg cgctctaccc tcataccggt aaagctcacc tgattggtgt
     3301 cactggtgcg cccggg
//
LOCUS       DBNHOH010000057         2659 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_2708, whole genome shotgun sequence.
ACCESSION   DBNHOH010000057 DBNHOH010000000
VERSION     DBNHOH010000057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_2708"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2611)
                     /locus_tag="AC16V7_00985"
     CDS             complement(<1..2611)
                     /locus_tag="AC16V7_00985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR6255718.1"
                     /translation="MAFNFPYDGINLHSLAGDFEIEPGSPAAGILREGDRILLVNDVP
                     LIEALPLYPGKIPGDLVILTVERDGSPIELTFKLEEPSYIERLYRLVPFLVALIFWGI
                     GVGVQAFKPAQEGSVIFFLFFQIFATALSAGSSSQFAPLWTAQLLSFLLWVGGPLMVH
                     FHFNFPQSLNIKSQRVFLAGLYSVGLVFGLLYAYVGSGEARSYWANQLFIASRFFLAL
                     CFLITLWLLFYSYRNATTAGVRSKIRIVVLGGAISLLPMITMGILPNALLGDTFIPQP
                     FLFLFLGILPLTYGFAILRYRLIEIERHVNRGASIILVYSILGVIYLLMSVGLDRFLP
                     NMPFKGPVINTVLVLILATIFSPLNRFVQRVVDIIFYGGWYDYRSAVSLITQGLEQIT
                     DLQSLANNTSARLLDTIRLEEVCVFLADLEGDFSVISVSPNPPTGEMHRPAYAPLPRS
                     SLSYLLNMGGAVERSVLRESLSQTVLSPQELSLLNSEQAYLWVPIIGHREVLGLMALG
                     PKLGGDLFSGEDTDILRVVARHLAPLIENLHLVTRLRLHNAMLEQRVTERTEELSASK
                     KRLEAILGSVGEGVVVIDLDGSLLSVNAAYEAQTGYNEQELFGQKLWGLYKIDKADNF
                     YSEIQGILQAGAVWTKEMVGLRKNGSQYDVQMTFAPLRDENGQIVGYVGSQRDITHQK
                     ELDRLKDMFVSDVSHELRTPTTNIGLYRELLLDAPPEKRTHYLNVLKEQSNLLIKLVE
                     DILDLSRLAFGKEKKVEFARIKLNKLVDQVVTAIRPLAEAGGLELSFNPDPNLPLVMG
                     EPNQLSRLISNLVANAIHYTVKGDVVVRTSWGDSMVCLEVHDTGIGIDPEDRPHIFER
                     FYRGKLVRQSKIHG"
BASE COUNT          784 a          679 c          573 g          623 t
ORIGIN      
        1 tgccatgaat tttactttgt cgtactaatt tacctcgata aaacctctca aatatgtgcg
       61 gtctatcttc cggatcgata ccaatgcccg tatcatgcac ctccaggcat accatgctgt
      121 caccccagga agtgcggaca actacatcac ctttgacagt gtagtggatg gcatttgcta
      181 ccaagttgct tattaaacgg gaaagctggt tgggttctcc cataaccaaa ggcaggttgg
      241 ggtcagggtt aaatgataat tctaagccac cagcttctgc caacggacga atggctgtga
      301 ctacctgatc aaccagcttg ttcaatttaa tccgggcaaa ttcaactttc ttttcttttc
      361 cgaaagccaa cctggataga tcaaggatat cttcgaccaa tttaattaac agattactct
      421 gttccttaag gacgttcaaa tagtgggtgc gtttctccgg tggggcatcc aacaacaatt
      481 cacgatacaa gccaatattg gttgtggggg tgcgtaactc gtgagacaca tctgaaacga
      541 acatatcctt caagcggtca agctcttttt ggtgagtgat atccctctgg ctaccgacat
      601 atccaacaat ttgaccattc tcatcacgaa ggggagcaaa cgtcatctga acatcatatt
      661 gactgccatt tttcctaagg ccaaccatct ccttggtcca taccgcgccc gcctgcagta
      721 tgccctgtat ttcactgtag aaattatctg ctttgtcaat tttatagagt ccccacagct
      781 tctgcccgaa tagctcttgc tcgttatagc cggtctgcgc ctcgtaggca gcattaacgc
      841 tcagcaaact accatccaag tcaatgacca ctacaccctc gccaacgcta ccgagaatgg
      901 cctcaagacg ttttttggaa gccgaaagct cttcagtacg ctctgtcacc cgctgctcca
      961 acatggcatt gtgaagccgg agtcgcgtga ccaggtgaag gttttctatc aatggtgcca
     1021 aatggcgtgc aaccacacgc aggatgtccg tatcctctcc actaaataaa tctccaccta
     1081 acttcggtcc caaagccatc aacccaagca cttccctgtg cccaattatc ggcacccaaa
     1141 ggtaagcttg ctcgctgttt aaaagagaaa gctcctgagg cgataacacc gtttgactga
     1201 gtgactcgcg tagcacggat ctttcaaccg cgccacccat attgagcaaa tatgataggc
     1261 tgcttcgagg cagtggggcg taggcaggtc gatgcatttc gccagtgggt ggattaggcg
     1321 agacggatat aacagagaaa tcgccctcta ggtcagcaag aaaaacacag acctcctcta
     1381 gccgaatggt gtccagcaag cgcgcgctcg tgttgtttgc caaggattgc aagtctgtaa
     1441 tttgctctaa tccttgggta atcaacgaaa ctgcactgcg atagtcatac caaccgccat
     1501 aaaatataat gtcaacaaca cgctgtacaa agcggtttag ggggctgaag attgttgcca
     1561 ggataagtac cagcacggtg ttgattactg gtcctttaaa cggcatattg gggagaaaac
     1621 gatctaaccc aactgacatg agcagataga tcacacccaa gatcgaatag accaggatga
     1681 ttgatgcgcc acgattgaca tgccgctcaa tctcgatcag gcggtaacgc agtatggcaa
     1741 agccataggt aagcggcagt attcctaaga atagaaagag aaatggctgc ggaataaacg
     1801 tatcacccaa cagtgcatta ggcaagatac ccatcgtaat catgggcaga aggctaatag
     1861 ctccccctaa aacgacaata cgaattttac tgcgcacccc agctgttgtg gcgttacggt
     1921 aagaataaaa caataaccat agcgtgatca agaaacataa agcaaggaaa aaccggctgg
     1981 cgataaacaa ctggtttgcc caataactcc tcgcctctcc gctgcctaca taagcataca
     2041 gcaagccaaa gacaagaccc acgctatata aaccagcaag aaatacacgc tggcttttta
     2101 tgtttagact ttggggaaaa ttaaagtgaa aatgaaccat taagggtccg cccacccaaa
     2161 gtaaaaaact gagcagttgg gctgtccata atggcgcaaa ctggctacta cttccagcac
     2221 tcaaagccgt ggcaaatatt tggaagaata agaagaatat aactgaaccc tcttgagctg
     2281 gcttgaacgc ctgtaccccc acgcctatgc cccaaaagat cagggcaact aaaaaaggca
     2341 ctaagcggta tagacgctcg atataggagg gttcctccaa cttaaaagtc aattcgattg
     2401 ggcttccatc cctctctacg gtaaggatta caagatctcc aggtattttc ccgggataaa
     2461 gtggcagcgc ttcgatcaac ggcacatcgt ttactaatag aatacgatcg ccttcgcgca
     2521 aaataccggc agctgggctg ccaggctcga tctcaaaatc cccagccagg ctatgcaaat
     2581 taataccatc gtaggggaaa ttaaacgcca aactggcacc ccaaattaaa taaacaaaca
     2641 ctgccaatgc gccaataaa
//
LOCUS       DBNHOH010000058         2142 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_323034, whole genome shotgun sequence.
ACCESSION   DBNHOH010000058 DBNHOH010000000
VERSION     DBNHOH010000058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_323034"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="AC16V7_00990"
     CDS             complement(<1..1106)
                     /locus_tag="AC16V7_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012966992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase C-terminal domain-containing protein"
                     /protein_id="HIR6255719.1"
                     /translation="MNDRVAIIGVGWAGFRPITPDLSYKELIYEAAVKAYSDAGVDPR
                     RDVQSFVTVAEDFHEGTSIFDEYTPDQIGAALKPVHTITGDGLHGLATGAMLIRTGMF
                     DLVAIEGHSKASNILTLPEVTAYAQDPVLNRPLRLNTHFVAGMEMNRYLYETGTTPEQ
                     CAAVVVKNRRNALRNPSAPYGADLTLEEVLSGQPLSWPLNRRETAEHSDGAIVMVLAS
                     EEKARALSDNPIWILGLGWINDSPSLEYREWGQLPYLQKAARMAYQQAGIRSPIGVVD
                     FAEVDDLYAYKELQSLEALGLCGVGEAGLITEEGLTEADGELPVNISGGSLGMGNLLD
                     ATGLSRALEVVLQLRGEAGPRQLENAEIGLAQSWR"
     gene            complement(1103..1993)
                     /locus_tag="AC16V7_00995"
     CDS             complement(1103..1993)
                     /locus_tag="AC16V7_00995"
                     /inference="COORDINATES: protein motif:HMM:NF026954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HIR6255720.1"
                     /translation="MRARRALLYMPGDDFHKIRKATTLGVDCICMDIEDGVAFNRKGE
                     ARKTILEALHTLDFGQAERLVRINPVGSGLELEDLLAGLEGHPDGIVVPKVENSDQVR
                     WASQSIASIEKKFGWPAGEIILIAIVETASGIINLPHIAAADPRLQALIFGAEDLAGD
                     IGAIRSHEGWEVFYARSAVVTHAAAFNLQAIDMVYLDFQDVDGLVQECQLGARMGFSG
                     KQVIHPNQVEPVQSAFTPSDEAISHALRLMEAYAAHQQSGRGAFALEGKMVDAPIIKT
                     AERVLERARAAGKILKGGQA"
BASE COUNT          512 a          584 c          539 g          507 t
ORIGIN      
        1 cgccaggact gggcgaggcc gatttcggcg ttctcgagtt gccgaggacc agcttcacct
       61 cgcaattgta agacgacctc cagggctcgc gagaggccgg ttgcatcgag cagatttccc
      121 atacccaaac tcccgccgga tatattcaca ggcaattcgc catccgcttc ggtcaagcct
      181 tcctcggtga tcaatcccgc ctcacctaca ccgcataaac ccagagcctc aagagattgc
      241 agctctttat aggcatagag gtcgtctacc tcggcaaaat ctacgactcc aattgggctg
      301 cgaatgcccg cttgctggta ggccattcgt gctgcttttt gtaggtatgg gagctgtccc
      361 cactcgcggt attccagaga gggtgaatca ttgatccagc caagacctaa tatccagatt
      421 ggattatcag acaaggcgcg cgctttctct tcggaggcca ataccatgac gatagcgcca
      481 tcggaatgtt cagcggtctc ccgccggttc agcggccagg aaagaggttg tccggaaagc
      541 acctcttcga gggttaaatc tgcaccataa ggagcggatg ggttgcgtag tgcattgcgg
      601 cggttcttaa ccacgacagc ggcgcactgc tcaggagtgg tgcctgtttc gtataggtat
      661 cgattcatct ccattccagc cacaaaatgg gtgttcaggc gcagtggtcg attgagcacc
      721 gggtcctgtg cataggcggt tacttctgga agggtaagaa tattcgatgc tttgctatgt
      781 ccttcaatag caacaagatc gaacatccct gtacgaatga gcatggcacc agtcgccaaa
      841 ccatgcaagc catctccggt aattgtatgc actggtttta gcgctgctcc aatctgatca
      901 ggtgtgtatt catcgaagat gctcgtccct tcgtgaaagt cttcggcaac cgtaacaaag
      961 ctttggacat ctctgcgtgg atcaacgcct gcatcggaat aagctttaac ggctgcttca
     1021 tagatcaatt ctttatagga tagatcgggt gtgatggggc ggaaacctgc ccatccaacg
     1081 ccgatgatcg ctacgcgatc attcatgctt gacctccttt caaaattttt ccggcagcgc
     1141 gtgcccgctc caagacacgc tcagcggttt taataatggg cgcgtcgacc attttccctt
     1201 caagtgcaaa tgccccacgc ccggactgct gatgagctgc atacgcctcc atgaggcgta
     1261 atgcatggga tatagcctcg tcactcggtg taaatgctga ttgaaccggc tcaacttgat
     1321 tggggtggat aacttgttta cccgaaaacc ccattcgagc gccgagctgg cattcttgaa
     1381 cgagcccatc gacatcttga aaatcaagat aaaccatgtc aatagcttgt aaattgaagg
     1441 ctgctgcatg tgttacgaca gcactgcgtg cataaaatac ttcccaaccc tcgtggcttc
     1501 gaattgctcc aatgtcacct gccaagtctt ctgcgccgaa tataagtgcc tgaaggcgcg
     1561 gatcggctgc ggcaatgtgc ggcagattga ttattcccga ggcggtttct acgatggcaa
     1621 tgagaattat ttccccagct ggccatccga atttcttttc tatcgaggcg atggattggc
     1681 tagcccagcg aacctggtca gagttctcga ccttgggaac gacaatccca tcggggtgac
     1741 cttctaaacc tgcaagcaag tcctccagct ctagcccaga gcccacaggg tttatacgga
     1801 caagacgctc cgcttgccca aaatcgagcg tatgtagcgc ttctaaaatc gtcttgcgcg
     1861 cctcaccttt ccgattgaag gcgacgccat cctcaatatc catgcaaatg cagtccacgc
     1921 ccaaggttgt ggccttgcgg attttatgaa aatcgtcacc cggcatatag agaagggcgc
     1981 gccgcgcgcg catgctcacc tcccgcgtcg agccagatta gccagaaaaa tactaaggaa
     2041 atacaagaca gtcatgggcg ccatcaccag ggtcatattg acaggatcaa cagtaggggt
     2101 aatgctggca gcaaggatag caattactac aactgctagt cg
//
LOCUS       DBNHOH010000059         2823 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1476680, whole genome shotgun sequence.
ACCESSION   DBNHOH010000059 DBNHOH010000000
VERSION     DBNHOH010000059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1476680"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..>2823)
                     /locus_tag="AC16V7_01000"
     CDS             complement(89..>2823)
                     /locus_tag="AC16V7_01000"
                     /inference="COORDINATES: protein motif:HMM:NF012618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255721.1"
                     /translation="ESWAAEHPQELSQNDQDFLEACQQLRTREEEAREAAERERRLKL
                     EAAEKVAEAERRRAEEQALAAKKLRTRFILAMLAFIAALFFAGAAYYSGQQAMVARNF
                     AYRESTRAVGNAATAEANAAIAATNEFKAVAAEATANSEREIAEKARSTAVAAEKDAL
                     NQKATAEYNAAIAQQQANLARSRELSNLSLSFIQDQPNLALLLSIEAYRKSDTGQALD
                     ALLSTLQRNLNREIQRSNVQIETQPISIDAMAISPDGHQLAWAGVQGLIRVYDFKLQK
                     QIWQDTIGIKINQLVFSPDGKILASADDTGEIIFWNPADGKKVRVIRPEIASIYAMDF
                     SPDGTSLAYGGISQSNKVNLFALNLNNLAIKNFSVRPLKTDILSLAWSPDGRLLASAG
                     RERVVRIWDVATEKELVTFEIYEGPMKSVVFSPNGQWLATGGNDEILPFDKNILLWDI
                     SLCDRPTDSANQGEQISPWDDPTCRLQLPVSFSGQEADVTSLAFSPDGLVLASGDISG
                     NTRLWDVAYRKPIDLQIPRARGIISQLAFSQIEDNLVLAVGSLDRTISLNNLRNTDSL
                     MTENTTTQSAIGGMAFRADGTFQVIGSDGAQTVLQTENEAGEVNQTGTFAPTSAVYAL
                     SKDTGSFAVLVEDNQEGRIEIWSEDDDSGPLLTIPSPAGDVITFSQAPNGQAISETLS
                     TSVGTIRSLALSPDGATLAAGVCAVSEKFIDPRLQASRTQAAGTERDVCTRNEIHLWQ
                     TGSGNLITQIPTDHRNAILSLAFSADGRILASGGADWTIYLRDIQEGRPIGLPLTGQA
                     GSVTALAFSPDGSLLASGSSRSLVALWNVASAQLIGDPIAGMSSIVTALAFSPDNKTL
                     VSGSDEAEVAWWRLDAWLDLACKYSQRNLSQQEWEQFFKGEPYRQTCPEYPVGE"
BASE COUNT          524 a          765 c          829 g          705 t
ORIGIN      
        1 ccgctggcgc cgtcaatacc gttaaaaacc aggctatcga aggtttcgtc ctcagagatg
       61 gtattgtttt cgttcacagt tgctccttct actctcctac cggatattcg gggcaagttt
      121 gccggtaggg ctcgccttta aagaactgtt cccactcttg ctggctcaag ttgcgctggg
      181 aatatttgca tgccaggtca agccatgcgt ccaatcgcca ccaggccacc tcggcttcgt
      241 cgctgccgga gaccagggtt ttgttatctg ggctgaaggc cagcgcagtg acgatgctgc
      301 tcatgccggc aatcggatcg ccgatcaact gggctgaagc gacattccac agcgccacca
      361 ggctccttga gctgcccgaa gccagtagag agccatctgg gctgaaagcc agggcggtga
      421 ccgagccagc ttgaccggtg aggggcaggc caattggtct tccctcttgg atgtctcgca
      481 agtagatcgt ccaatccgca ccgccgctgg ccaggatgcg cccatcggcg ctaaaagcca
      541 ggctgaggat ggcgttcctg tgatcggtgg ggatttgcgt gatcaaattg cccgagccag
      601 tctgccagag atggatctca ttgcgggtgc acacgtcgcg ctcagtgccc gcagcctgtg
      661 tgcgcgaggc ttgcaggcgc gggtcgatga atttttcgct tacagcgcag accccggctg
      721 ccagggtggc gccatctgga ctgagggcca ggctgcggat tgtcccgacc gaggtgctca
      781 aggtctcgct gatggcttga ccattggggg cctggctgaa ggtaatcacg tctccagcgg
      841 gagatgggat tgtcagcaat gggccgctgt cgtcatcttc gctccagatt tcgatgcggc
      901 cttcctggtt atcttctact aaaactgcga atgaaccagt atccttgctc agagcataga
      961 ccgcggatgt tggcgcaaag gtgcctgttt ggttgacttc tccagcctcg ttttctgtct
     1021 ggagcaccgt ttgtgcgcca tcgctgccga tgacctggaa agtgccatcc gctctaaagg
     1081 ccatgcctcc aatagcgctt tgggtggttg tgttttcagt catcaaagag tcagtgttgc
     1141 gcaggttgtt caggctgatg gtgcggtcta gactgcccac ggctagcacc aggttatctt
     1201 ctatctggct gaaggctagc tggctgatga tacccctcgc ccttggtatt tgcaggtcga
     1261 ttggctttcg gtaggcgaca tcccataagc gggtgttgcc ggagatatcg ccagatgcca
     1321 gcaccaggcc gtctgggctg aaggcgaggc tggtgacatc cgcttcctgg ccagaaaatg
     1381 ataccgggag ttgcaggcgg caagtcgggt catcccaagg gctgatttgt tcaccttgat
     1441 tggcgctatc tgtaggccgg tcgcatagcg agatatccca caacaggata ttcttgtcaa
     1501 atgggaggat ctcatcatta cctccggttg ccagccactg gccgttgggc gagaaaacca
     1561 cgctcttcat cggcccttcg tatatctcaa atgtgaccag ctccttctcg gtggctacgt
     1621 cccaaatgcg cacaacgcgt tcgcgcccgg cagaggcgag cagcctgcca tccggcgacc
     1681 aggcgaggct caagatatcg gtcttgagag ggcgcacgct gaagttcttg atggcgaggt
     1741 tatttaagtt cagggcaaac aggttgactt tgtttgactg ggagatgcct ccataagcca
     1801 ggctggttcc atctgggctg aagtccatgg catagatcga ggcaatttct ggacggatga
     1861 ctctcacttt ctttccatcc gctggattcc aaaatatgat ctcacctgtg tcatcggcgg
     1921 aggccaggat tttaccatca gggctgaata ccaactggtt gatcttgatc ccaatggtgt
     1981 cttgccagat ttgtttctgt agtttgaaat catacacgcg gatcaaaccc tgcacgcccg
     2041 cccaggctag ttggtgccca tcagggctga tagccatggc atcgatcgag ataggctgtg
     2101 tctcaatttg tacattggat cgttggattt ccctgttcag gttgcgctga agcgtgctga
     2161 gcaaggcgtc cagagcttgc ccggtatccg atttgcggta tgcctcgatg ctcagcaata
     2221 atgctagatt cggctggtct tgaatgaagc tgagcgaaag gttggaaagc tcacgggagc
     2281 gagccaggtt agcctgttgc tgtgcgatcg cggcgttgta ctcggcagtc gctttttgat
     2341 tcagtgcgtc cttctcggcg gcaactgcgg ttgatctggc cttttcggcg atctcccgct
     2401 ccgagttagc ggtcgcctca gcagctacgg ctttaaattc gttggttgcg gcgatggcag
     2461 cgttggcttc ggcggttgca gcgtttccga ctgcccgcgt gctttctcta taagcaaaat
     2521 ttctggcgac cattgcctgt tggccactgt aataggccgc gcctgcgaag aatagggcgg
     2581 caatgaaggc cagcatggct aaaataaagc gcgtgcgtaa tttttttgcg gccaaggctt
     2641 gttcttcagc gcggcgtcgc tctgcctctg ccaccttttc ggctgcctcc agcttgaggc
     2701 gtcgctcgcg ctcggcagct tctcgggctt cttcctctcg cgtgcgcagt tgctggcagg
     2761 cttccagaaa atcttgatcg ttctggctga gttcctgggg gtgttccgca gcccaggatt
     2821 cgg
//
LOCUS       DBNHOH010000060         5481 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1028099, whole genome shotgun sequence.
ACCESSION   DBNHOH010000060 DBNHOH010000000
VERSION     DBNHOH010000060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5481)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1028099"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="AC16V7_01005"
     CDS             <1..213
                     /locus_tag="AC16V7_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255722.1"
                     /translation="GKDVYGLSINGTCKRWTNPPRGWGFLSKAIGLLITALAARLGAP
                     FWFEILRKLVNVRSTGIKPEEKREKK"
     gene            complement(276..1961)
                     /locus_tag="AC16V7_01010"
     CDS             complement(276..1961)
                     /locus_tag="AC16V7_01010"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004467 - long-chain fatty acid-CoA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HIR6255723.1"
                     /translation="MDERPWLKRYDTDVPHQIDYPKVPLFALLEEAAQKYADAPCTIF
                     KGARITFREMNEITDRLAAGLAELGVKKGDRVGIFMPNTPQFVMAYFAILKVGGVVVA
                     TNPLYSGREIEHQVNDAGIEVMLVMSNFYNIIKQVQPKTKIRTVVVTNLKETLPPVLA
                     FLFTLTREKKGGFRVQLAEGDIWMQDLIARHKPEERPKVEVGPEDVALFQYSGGTTGI
                     SKGAIAPHRNLVANALQIRSWITNAEDGKETTLMAIPLFHVYGMVAGMLFSIRTGAAM
                     VMVPNARDLKDVLTNIQKYQTSIFPGVPTLYNAINNHPDVAAGKYNLSSVKACISGSA
                     PLMRETKEKFETLTGGKLFEGYGLSEAPTATHCNPLMGENRTGSIGLPLPDVECRIIS
                     LDDEVTVLPVGEIGELVVKGPQVFKGYHNMPTESANTLREGWLYTGDIARMDEDGYFY
                     IVDRKKELIKPGGFQVWPREVEEVISAHPKVMEVGVGGIPDPYRGETVKAWVVVNPGE
                     TLTEEEVRDWCKERLAKFKVPTHVEFRTELPKTTVGKILRRELVRQHKETAGE"
     gene            complement(2078..2356)
                     /locus_tag="AC16V7_01015"
     CDS             complement(2078..2356)
                     /locus_tag="AC16V7_01015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6255724.1"
                     /translation="MPYGPGWVLVGDAAQILDPWSGQGIDHAGTHAVMLVEALHTFLS
                     EEAPWDAAMAGYHSSRNNWSRKTYQRTSSYAHDLRPMTRAALVKRGIA"
     gene            complement(2419..2667)
                     /locus_tag="AC16V7_01020"
     CDS             complement(2419..2667)
                     /locus_tag="AC16V7_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255725.1"
                     /translation="MDRYLIETPHTDEDCQRLIDNVYAMGYIYHFDWGCDAGVHCGWA
                     IIEAEDEEQARLAVPSLVRNKARVIKLKKYTPETLDDH"
     gene            2867..3628
                     /locus_tag="AC16V7_01025"
     CDS             2867..3628
                     /locus_tag="AC16V7_01025"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HIR6255726.1"
                     /translation="MNNSFYIPESALIIVAHPDDIEFSCAGTLARWAQAGTRLCYVLC
                     TSGDVGIAEPGMTKAQAIAIREAEQLQAAAIVGTKDVVFLREPDGMLVPTIELRKKLV
                     REIRRFRPEVVVCGDPTIVWVGNDYINHPDHRAAASAALDAIFPAAGQPNLFEELEEE
                     GIHAHKPRKVYVTGWVQHELYVNITETIDIKIAALRAHKSQMQDWDPEPRIKEWAAER
                     AKGKEMVYAETFRVVTLVEDEEWQKTNGRVLPDKS"
     gene            3709..4389
                     /locus_tag="AC16V7_01030"
     CDS             3709..4389
                     /locus_tag="AC16V7_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR6255727.1"
                     /translation="MSLPLHALLVMAKRPSPGRTKTRLSPPLSPKAAGGLYECFLLDT
                     LDLVRQVPDVHKVIAFTPKSASGYFSKLAADFELIPQTGSNLGSRLDNALSYYLLNGF
                     TQVVIMNSDGPTIPVEYLIKAHKALAQVDVVLGPSEDGGYYLIGLKKPAPRLLRDVHM
                     STPRVTSDTLRIADSLGLRVHLLPGWYDIDDVETLDRLLVDLEQSPRLIAPQTRRYLK
                     QINMLSTR"
     gene            4386..5081
                     /locus_tag="AC16V7_01035"
     CDS             4386..5081
                     /locus_tag="AC16V7_01035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR6255728.1"
                     /translation="MSEIAVIIPALNEAGNLFRLIGEVRSTVNADIIVVDNGSTDETA
                     DEAIRAGAQLIQEPRRGYGYACAAGVAASEGSEILVFLDGDFSSLPSEIPALVNPIQS
                     GEADLVQGSRVLGHIAAGAMPFQQRFGNWIASRLMNFLYGSRITDLGPFRAIRKSLLS
                     TLNMREMTYGWPTEMTVKAQRRGARIKEVPVSFHPRQAGHSKISSTLRGTILAGWYIM
                     GVTLRYAWGSWDD"
     gene            complement(5078..>5481)
                     /locus_tag="AC16V7_01040"
     CDS             complement(5078..>5481)
                     /locus_tag="AC16V7_01040"
                     /inference="COORDINATES: protein motif:HMM:NF019121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR6255729.1"
                     /translation="PRIQSGLRFFPPMEVNLKPFEDSDVVYSGRLKVDMADQLDYLCP
                     APLDSDMVDELNWLTAAVFRVTGAMDVSRVDFRLDVHDNWKPYILEINPLPGLSPGIS
                     DLVIEAAAEGVDHTYLVNMILDTALERYGMI"
BASE COUNT         1345 a         1455 c         1342 g         1339 t
ORIGIN      
        1 ggtaaagacg tatacggtct atcgattaat ggcacctgca aacgctggac caacccacca
       61 aggggttggg ggtttctaag caaagctatc ggcttattaa tcaccgccct agcagcccgg
      121 ctgggcgcgc ctttctggtt cgaaatcctc cgaaagctgg tcaacgtgcg ctcaacgggt
      181 ataaaacccg aagagaaaag agagaagaaa taaaaacggg ttatcggacg gcgtaactgt
      241 cgcccaataa cccgcgttgt ttcgctaatt aaagtttatt ctcccgctgt ttctttgtgc
      301 tggcgcacca actcacgccg caggattttg cccacagtcg tctttggcaa ttcagtgcga
      361 aattccacat gcgtcggcac tttgaatttt gccagccgtt ctttgcacca atctctgact
      421 tcctcttcgg tgagagtctc gcccgggttg accactaccc atgcttttac tgtttcgccg
      481 cggtatgggt cagggatgcc tcccaccccc acttccatca ctttcgggtg agctgaaatc
      541 acttcctcca cctcacgggg ccaaacctgg aaacctccgg gcttgatcag ctctttctta
      601 cgatcaacga tgtaaaaata tccgtcctcg tccatacgag cgatatcgcc agtatacagc
      661 cacccttcac gaagcgtatt tgcggattcc gtgggcatat tatgataccc tttgaaaacc
      721 tgcggacctt taacgaccag ctcaccgatc tctccgaccg gcaaaaccgt cacctcatcg
      781 tcaaggctga taatacggca ttccacatca ggcaagggta aaccaatcga gccagtgcgg
      841 ttttcaccca tcaatggatt gcaatgtgtg gcagtgggag cctctgaaag tccatacccc
      901 tcaaagagtt taccgccggt aagcgtttcg aatttctctt tcgtctcgcg catgagtgga
      961 gctgagccag agatacatgc tttcaccgaa ctcaagttat acttccccgc tgccacgtct
     1021 gggtgattat taatggcgtt atacaatgtg ggcaccccgg ggaatataga ggtttgatac
     1081 ttctggatat tggtgaggac atccttcaaa tcacgcgcat tggggaccat gaccatcgca
     1141 gcgcccgttc ggatggagaa aagcatacca gccaccatcc catacacatg gaagagcggt
     1201 atagccataa gagtcgtctc ttttccatcc tcggcatttg ttatccagga gcggatctgg
     1261 agtgcattgg cgactaaatt tcggtgtggg gcaatggcgc ctttagaaat tcctgtggta
     1321 ccgccgctgt actgaaaaag tgccacatcc tcaggaccaa cttctacttt cgggcgctcc
     1381 tcgggtttgt gccgggcaat caaatcctgc atccaaatat cgccttcagc caactgcacg
     1441 cggaatccac ctttcttctc gcgtgtcagc gtgaacagaa aagccagaac tgggggcagg
     1501 gtttccttca aattggtcac cacgacagtt cgtatcttcg tctttggctg tacctgcttg
     1561 atgatattgt aaaaattgct catcacaagc atcacttcaa tgcccgcgtc gttgacttgg
     1621 tgctcaatct cgcgcccact gtacagtgga ttggttgcca caaccacccc acccaccttg
     1681 aggatagcaa aatatgccat cacaaattga ggcgtgttag gcatgaagat gcccactcga
     1741 tcgccttttt tcactccaag ttctgctaaa cccgccgcta gccgatcggt gatctcattc
     1801 atttcgcgga atgtgattct ggctcccttg aaaatcgtac agggagcatc agcatacttc
     1861 tgagctgctt cctcaagcaa tgcaaacagg ggaaccttag ggtagtcgat ttgatgcggc
     1921 acatccgtat cgtatcgctt aagccacggt cgttcgtcca tatcctcctc caaatgttgt
     1981 gagacaaacc gatttaggat aaatgatcac agatagtata gagtgaacca cctggaaagt
     2041 caatcggttt ttggaacccc catcaaagca ggtcctgtca ggcgattccg cgcttcacca
     2101 gggctgccct tgtcatgggc cttaggtcgt gagcgtaaga gctggtgcgt tggtaggtct
     2161 tacgactcca attattacga gacgaatggt atccagccat tgccgcatcc caaggtgctt
     2221 cttcgctcag gaatgtgtgg agagcctcta caagcatcac cgcatgcgtt cctgcatggt
     2281 caattccctg acccgaccag ggatccagta tctgagccgc atctccgacc aacacccatc
     2341 ccggaccata aggcacgcgt tgataacgtg gaaatgcgtg taatacatgg ctctttgcac
     2401 tttctgttat cttgcatcct agtggtcgtc aagagtctcg ggggtatatt ttttcaactt
     2461 gatgacgcgc gctttgtttc gcaccagtga agggactgcg agccgagcct gttcttcatc
     2521 ttctgcttcg ataatcgccc agccgcaatg cacaccagcg tcgcaacccc aatcgaagtg
     2581 gtaaatatac cccattgcat acacgttatc gatcagcctt tggcaatcct catcggtgtg
     2641 aggggtctcg atcaagtatc tatccatttc cacctccttc tattggtact aatatactcg
     2701 gtaatattcg tgaatataaa aatgatgaca caacagagaa tagtggagta aaccactcca
     2761 ctttagagtt taacccatac accattttat tgcaatacac atttattgct tgaatcaggc
     2821 ccaagcgtta gaataggggc tgtgatctaa ctggaggagt ccaaatatga ataacagttt
     2881 ttatatcccc gagagtgctt tgatcatcgt agcgcaccca gatgatatcg aattctcctg
     2941 tgctgggaca ttggcgcgct gggctcaggc gggaactcgc ctgtgctacg tactctgcac
     3001 aagcggtgat gtagggatcg cggagccagg tatgacaaaa gcacaagcta ttgccatccg
     3061 tgaggcagaa cagctccaag ctgcggcgat cgttgggaca aaggatgtgg tctttttgcg
     3121 cgagccggat ggtatgttgg ttcccacaat tgaattacgg aagaagttgg ttcgagaaat
     3181 tcgccgcttc cgacccgagg ttgtcgtgtg tggtgatccg accatcgttt gggtcggtaa
     3241 cgactacatc aaccacccag accaccgagc cgctgcctcc gcagctctgg atgcgatatt
     3301 ccctgccgct gggcaaccca acctgtttga agaactcgaa gaagaaggca tacacgctca
     3361 caaaccgcga aaagtatatg tgaccggatg ggtgcagcat gagttgtatg tcaacatcac
     3421 agaaactatt gatatcaaaa ttgcagcgct gcgcgcccac aagagccaaa tgcaagattg
     3481 ggatcccgaa ccacgcatta aagaatgggc tgcggagcgc gctaaaggga aggaaatggt
     3541 ctacgcggag acttttcggg tagtaaccct agtggaagat gaagaatggc aaaagaccaa
     3601 cggccgcgtt ttaccggata agtcatagcg atttgattcg actcgggtta gtatgatcac
     3661 ctcaatggca caaaacactg tttcggctat gttttaagat aattgtgtat gtcgctcccc
     3721 ctgcatgcct tattggttat ggccaagcgc ccctcacctg ggagaactaa aacacgcctc
     3781 agcccaccgc tctctcccaa agcggctggc gggctgtatg aatgttttct actggatacg
     3841 ctggaccttg ttcgccaggt acctgatgtg cacaaagtta ttgcgtttac gccaaaaagt
     3901 gcttcaggct acttcagcaa gctagcagcc gactttgagc tcataccaca aaccggatct
     3961 aatctgggca gccggctaga caatgcgctt tcttactatc tccttaatgg cttcacacaa
     4021 gtcgttatca tgaacagcga tggccccacc ataccggtgg agtacttgat caaagcccac
     4081 aaagccctcg cacaagttga cgtagtcctg ggtccaagcg aagacggtgg ttattactta
     4141 attggactga agaagcccgc accccgcttg ctgcgcgatg tgcatatgag cacgccccgt
     4201 gtcacttcag acactttaag aattgccgac agcttggggc tgcgtgttca tctgttaccc
     4261 ggctggtacg acatagatga tgtcgagacc ttggatcgac tgctggttga tttggagcaa
     4321 tcaccccgac taatagcccc gcagactcgc cggtacctca aacaaatcaa catgttatca
     4381 actcgatgag cgaaatcgcc gttattatcc cggctttgaa tgaggctggc aacctctttc
     4441 gcttgatcgg cgaagtgcgc tccactgtaa atgcagacat catcgtagtg gacaacggtt
     4501 ccacagacga aactgctgat gaagccatcc gcgcgggcgc acaactgatc caagaaccaa
     4561 ggcgtggata tggttatgcc tgtgctgctg gggtagctgc gtcagaaggt agcgagatcc
     4621 tggtctttct ggatggggat ttcagctctc tgccatcgga gatccctgcc ttggtaaacc
     4681 caattcaatc cggggaggct gacctggtgc agggctcccg cgttcttggg catatcgccg
     4741 ctggtgcgat gcccttccaa caacgttttg ggaattggat agcatctcgc ttgatgaact
     4801 tcctctatgg atcacggatt acggatttag gtccctttcg cgcgatacga aagtcgctgc
     4861 tctcaacact caatatgcga gagatgactt acggatggcc caccgagatg accgtaaaag
     4921 cccaacggcg aggggcacga attaaagaag tgccggtcag ctttcatccc cgccaggccg
     4981 gacattcaaa aatcagcagt acgttgcggg gaaccatctt agcaggatgg tatattatgg
     5041 gcgtaacttt gcgttatgca tgggggtcat gggatgatta aatcatccca tagcgttcga
     5101 gcgctgtatc taagatcata ttgaccaggt aagtatggtc tacaccctca gcagcggctt
     5161 cgatgaccag atctgatatc cctggagaca gacctggcaa cggattgatc tccaaaatat
     5221 aaggcttcca attgtcgtgg acatccaggc ggaaatcgac gcgcgagaca tccatggcgc
     5281 cggtcactcg gaaaaccgct gccgtgagcc agttcaattc atcgaccatg tccgagtcca
     5341 gtggagccgg gcagagataa tccaattgat ccgccatgtc cactttgagc ctgccactat
     5401 ataccacatc gctgtcttca aacggcttca ggttgacttc cataggtggg aaaaagcgca
     5461 agcccgattg aatgcgcggg g
//
LOCUS       DBNHOH010000061         3733 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_900122, whole genome shotgun sequence.
ACCESSION   DBNHOH010000061 DBNHOH010000000
VERSION     DBNHOH010000061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_900122"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1431
                     /gene="tilS"
                     /locus_tag="AC16V7_01045"
     CDS             <1..1431
                     /gene="tilS"
                     /locus_tag="AC16V7_01045"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR6255730.1"
                     /translation="FLIMLARMSDVLHNYCQIDSALPVLLGVSGGPDSVCLLDMLSHL
                     GYRVVVGHFNHRLRDEAFDEARRVEELAAQMQVPFVSDSQDVASYARLKGLSIEEAAR
                     ELRYGFLFKQAEKLQVGSVMVGHTADDQVETVLMHLLRGAGLDGLKGMAYRSLPNPWS
                     QKIPLVRPLLAFWRQEILDYIDARELQATIDASNLDTTYFRNRLRNEVIPYLEKIQPQ
                     LRQLLWRTADIIRHENLLLDGLVEQAWSECVTQQGHGYLALHVASMQGLPLAMRRRLL
                     RTAINNLRPGLRDVGFDIIERGLAFIASPQTKGQCSLVGGLVLLKEEEIVWLSSWEAA
                     LPTASWPKMPGSQVIELHIPGRITLADGWLLQAVEVVASSQVREAIESNSDPFQAWFD
                     ADALPEILQVRSRRPGDRFKPLGMQGHSLKVSDLMVNLKVPQRARAGWPLVWAGEEII
                     WVPGCRQAQRGQVHGDSQRLLHLKLG"
     gene            1565..2545
                     /gene="moaA"
                     /locus_tag="AC16V7_01050"
     CDS             1565..2545
                     /gene="moaA"
                     /locus_tag="AC16V7_01050"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HIR6255731.1"
                     /translation="MAYDLFGRLIHYLRISLTDHCNLRCVYCMPEDITFRPTAELMQD
                     DEILTLVRVFASVGFDKFRLTGGEPTVRAGVVELVRRIVNTPGVRTLSMTSNGVLFSR
                     LAQPLAEAGLRRVNFSLDTLNPEKFRRLTRRATLAEVWEGIRAAEQAGLTPIKINAVV
                     VRDYSDADAVELARLTYDHPWQVRFIEMMPFAGATEFQRALVVTTEEIKTRLESALGH
                     LELVNGGKLDGEAQLYHLPGARGDIGFISTVSQPFCASCTRARLTADGRLRLCLLRED
                     EVDLLTPLRAGASTEELRQLILEAIWHKPWGNRLEQGEIPLNRAMSEIGG"
     gene            complement(2581..3471)
                     /locus_tag="AC16V7_01055"
     CDS             complement(2581..3471)
                     /locus_tag="AC16V7_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR6255732.1"
                     /translation="MSPEATSAHITSESLLIPTINAWRIFLDDQGNSVHTVKAFTADL
                     RLLATYLPPDRPLGDITTTDLNTFLEWMQSSRGVPCSPKTLARRITSLKAFFRWLHKN
                     GVILIDPAEKVPQKSVLSPLPEVLTPEEVEAVMDAADKQRRGAKPDARSYLLVSLLLE
                     TGIKKGECIALSPNHLDLEAPSGPHLFVRYASPQHRYKERKIPIPESWVNTLHEYKSQ
                     YEIVDRLFPWSPRRLEYLLEDLGEAAGLSKHLSFDMCRWTAALSDWQSGMDHDKIRQK
                     LGISKVQWREISMKLEKLAA"
BASE COUNT          834 a          869 c         1072 g          958 t
ORIGIN      
        1 ttcttaatca tgttggcacg tatgtccgat gtactgcaca attattgcca gatcgattcc
       61 gctctgcctg tattactggg tgtgtctggt ggaccggata gtgtgtgctt gctggatatg
      121 ctttcccacc tcggatatcg ggttgtggta ggacacttca accaccgttt aagggatgaa
      181 gccttcgatg aggctcgtcg cgtggaggaa ttggctgctc aaatgcaggt gccctttgtg
      241 tcggatagcc aagatgttgc cagctatgcc cgattgaagg gtctctcgat agaggaagct
      301 gcccgggagc tgcgctatgg tttcctgttt aagcaagcag aaaaattgca ggtagggtct
      361 gtgatggtcg gtcacactgc agacgaccag gtggagacgg tgttgatgca cctgctacgc
      421 ggcgcgggat tggatgggct taagggcatg gcttaccgat ctttgccaaa tccctggagt
      481 caaaaaatcc ccttggtacg tccgctgctg gctttttggc ggcaagaaat cttggattac
      541 atcgatgcgc gcgaactgca agcgacgatt gacgccagca atttggatac aacctatttt
      601 cgcaatcgcc ttcgtaatga agtcattcct tacctggaaa aaatccaacc gcaattgcgc
      661 caattattgt ggcgaactgc agatatcatt cgtcatgaaa acctgttgtt ggatggcttg
      721 gttgagcaag cctggagtga atgtgtgacc cagcagggac atgggtactt agccctacat
      781 gtggcctcta tgcaggggct gcctctggca atgcggcggc gtttattgcg aactgcgatc
      841 aataatttgc ggccaggttt gcgtgatgtg ggcttcgata tcatcgagcg cgggctggct
      901 ttcatcgcct caccccagac gaagggacaa tgcagcctgg ttggagggtt agtactcttg
      961 aaagaggagg aaatcgtctg gctgtcttcc tgggaagcag cgctgcccac agcgagctgg
     1021 cccaaaatgc caggctcgca agtgatagaa ctgcatatac ccggcaggat cactctcgca
     1081 gatgggtggc tacttcaagc ggtagaggtt gtggcaagta gccaggtcag agaggcgatt
     1141 gagagcaata gcgatccatt tcaggcttgg tttgatgcgg atgcgcttcc agagatattg
     1201 caggtgcgaa gccgccggcc cggagatcgt tttaagcctt tggggatgca agggcactct
     1261 ctgaaggtgt cggacctgat ggtaaatctc aaagtacccc aacgcgctcg cgcaggctgg
     1321 ccactagtat gggccggaga agagattatc tgggtgccag gttgccgcca ggcgcagcga
     1381 ggccaggttc atggagactc ccagcgtctg ttacatttaa aattgggata gcagaatatt
     1441 actatcggaa tttcggcaaa ttgaggagag tggggagatg aacaagcttc gctcacccaa
     1501 ctcccctact tcatcctaat tacgtgattt agagttataa tgtttctgtt tcaaggtgat
     1561 ttttatggcg tatgatctct ttggccgcct tatccactat ctgcgtatca gcctgactga
     1621 tcactgcaac ctgcgttgtg tttactgtat gccggaggat atcaccttcc ggcctactgc
     1681 tgagctcatg caggatgatg aaattctcac gctggtgcgc gtttttgcct cagtaggatt
     1741 tgataaattt cgcctgaccg gcggcgagcc aaccgtgcgg gctggtgttg tggagctcgt
     1801 acgacgcatt gttaatacgc ctggtgtgcg gacgttatcg atgacctcta atggggtttt
     1861 gttctctagg ctcgctcaac ccttagcgga agcgggcctt agacgggtga acttcagcct
     1921 ggataccctc aacccagaga aatttcgccg cttaactcgt cgagccacat tggcagaggt
     1981 ctgggaaggc atccgggctg ccgaacaggc tggactgacc cccataaaaa taaatgctgt
     2041 ggtggtgcgc gattatagcg atgccgacgc agtggaactg gctcgcctga cttacgatca
     2101 tccctggcag gtacgcttca tcgagatgat gccttttgct ggggcaacag agtttcagcg
     2161 ggcattggtg gtcactactg aagagatcaa gacccgcttg gaaagcgcgc ttggtcatct
     2221 ggaactggtg aacggcggca aattggatgg cgaagcacaa ctttatcatt tgccgggggc
     2281 gcgtggcgat atcggtttta tctccacggt gagccagcca ttttgtgcca gttgcacgcg
     2341 ggctcgccta accgccgatg gcaggctgcg cctgtgtctt ttgagagaag atgaagtgga
     2401 tttgctcacc ccgctgcgcg ccggggctag tactgaagaa ctgcgtcagc taatcctcga
     2461 ggcaatttgg cacaagccgt ggggcaatcg tctggaacaa ggtgagatcc ccctaaaccg
     2521 cgcgatgagt gaaatcggtg gataaaacaa tccggcgggc ttgcccgccg gattgtttta
     2581 tcaggctgcc agtttttcaa gtttcatgct aatttcgcgc cattgcacct tggatattcc
     2641 cagtttttgg cggatcttat cgtgatccat accggattgc cagtcgctca aggcggcggt
     2701 ccagcggcac atatcgaaag ataggtgttt gctcaagccg gctgcctctc ccaggtcttc
     2761 gagcaggtac tccagccggc gcggcgacca ggggaagagt cgatcaacga tctcgtactg
     2821 agatttatat tcatgtaggg tatttaccca gctctctgga ataggtattt tacgttcctt
     2881 ataccggtgc tgggggctgg cgtagcgcac aaacagatgc ggaccagaag gggcttccag
     2941 gtcaaggtgg ttgggactta gggcaataca ttcacctttc ttgatcccgg tttctaataa
     3001 cagagaaacc aggagataag aacgggcatc aggctttgcg ccgcgccgct gcttgtcagc
     3061 ggcatccatg accgcctcca cctcctcggg tgtgagtact tccggcagcg ggctgagcac
     3121 agatttttgg ggcacttttt cagcaggatc tatcagaatg acgccgtttt tatgcagcca
     3181 gcgaaaaaaa gcttttaagg aggtgatacg acgggcaagg gtctttgggc tgcagggcac
     3241 gcctcgcgat gattgcatcc attccaggaa ggtgtttagg tcagtggttg tgatatcccc
     3301 cagagggcga tcggggggta ggtacgtggc cagcaagcgc aagtctgcgg tgaacgcttt
     3361 aaccgtatgt acggagttgc cctggtcatc caggaagatg cgccaggcgt tgatggttgg
     3421 tatgagcagg gattcggagg taatatgagc agaagtagct tcaggtgaca tgatcacttc
     3481 caggtgaatt agattttggg tcgccagtta tgtttgtacg taagtatgat tataaaaatt
     3541 ttttcgtgta gtcgaaattt cgtatagttt aatatgattt agttgatttg tcaacttttc
     3601 gggtaaaatg ctttatatac ttcaaaatta tgcagtaatg aggagatagg ataatttttg
     3661 agagcgcgag ctgcccaaat taccctcatt taccctacag ctgcatatgt cctatacttt
     3721 acatttcgtg cag
//
LOCUS       DBNHOH010000062         1250 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_964316, whole genome shotgun sequence.
ACCESSION   DBNHOH010000062 DBNHOH010000000
VERSION     DBNHOH010000062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_964316"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="AC16V7_01060"
     CDS             complement(<1..827)
                     /locus_tag="AC16V7_01060"
                     /inference="COORDINATES: protein motif:HMM:NF013381.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HIR6255733.1"
                     /translation="MSMTSRERVQTALHHEEPDRVPIIIGASNATSMKLKPYLGLKDL
                     LGIQGEDRYLYDWPELGSIHPDERILQRLLSDVRGVLDRAPQAIYHRNRKRTAHTPYI
                     DDWGIGQEEIEPDVWFPSLHPMAAARSLDDIENYPWPDMHDPTRVAHVRQVAKRLSQE
                     NQYAIMATPWLLFPFERANALMGMEHFLPNLVENREFAVALLLKITELCKTLMGHFLS
                     ELGKDVDIIKIGDDLGMQSGLLISPRMYRQVLKPIHADYIAFIKAHTKAKVFFHTDG"
     gene            927..>1250
                     /locus_tag="AC16V7_01065"
     CDS             927..>1250
                     /locus_tag="AC16V7_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR6255734.1"
                     /translation="MSELKTLEIPISGMDCADCTLHVKRAIAALPGVEAVEVFLASEK
                     AIVSLDPRLVDLPAIRKAVAGAGYAVPQVDEHAKTSSLQDFTRPILTLLGVIFGVVLF
                     VVVVGE"
BASE COUNT          264 a          314 c          354 g          318 t
ORIGIN      
        1 tcaccatccg tgtgaaaaaa tactttggcc ttcgtgtgtg ctttgatgaa agcaatataa
       61 tcggcgtgta tcggtttgag cacctggcga tacatgcgcg gcgagatgag tagcccactc
      121 tgcataccca ggtcgtcacc gatcttgatg atatcaacgt ccttgccaag ctcgctcaga
      181 aaatgcccca tgagcgtttt gcatagctca gtgatcttaa gaagcagtgc cacagcaaac
      241 tcgcggtttt ccaccaggtt cggtaagaag tgttccatac ccatcaatgc gttggcgcgc
      301 tcgaatggga agagcagcca gggtgtagcc atgatggcgt attgattctc ttgtgacagg
      361 cgcttggcga cctggcggac atgtgccacg cgtgttggat cgtgcatgtc tggccagggg
      421 tagttttcga tatcgtccag gcttctggct gccgccatgg ggtgcagact gggaaaccag
      481 acgtcgggct cgatctcctc ttgcccaatg ccccaatcgt cgatgtaggg cgtgtgtgca
      541 gtacgctttc gattgcgatg atatatggct tgcggggcgc gatccagcac gccgcgcacg
      601 tcgctgagca ggcgttgtaa gatgcgctcg tcagggtgaa tcgagccgag ctctggccaa
      661 tcatatagat agcggtcttc accctggata ccaagcaggt cttttagccc aaggtagggt
      721 tttagcttca tcgaagtagc attgctagct ccgataatga tcggcacgcg gtccggctct
      781 tcgtggtgga gagcagtctg aaccctctct cgtgaagtca tgctcatatt aaatttttca
      841 gcctagtaga tttacaaagc acgcagagtt gatcgtgatc gagcaggatt aaattcttaa
      901 ctctgattgt aatataatct gtccatatgt ccgaactcaa gacccttgaa atcccgatca
      961 gtggcatgga ctgcgccgat tgcacgctgc acgtcaagcg agccattgct gctttgccag
     1021 gtgtggaggc ggtggaggtt tttctggctt ccgaaaaagc catcgtcagt ctagacccgc
     1081 ggctggtgga tttgcctgcc atccgcaaag ctgttgcagg tgcagggtat gccgtccccc
     1141 aggtggacga acacgcaaaa acctcatcct tgcaggattt tacccgacct atcctgacct
     1201 tgctgggggt tatctttggg gtggtgctgt tcgtggtagt ggtcggcgag
//
LOCUS       DBNHOH010000063         7100 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_259163, whole genome shotgun sequence.
ACCESSION   DBNHOH010000063 DBNHOH010000000
VERSION     DBNHOH010000063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7100)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7100)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7100
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_259163"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..1270)
                     /gene="galK"
                     /locus_tag="AC16V7_01070"
     CDS             complement(98..1270)
                     /gene="galK"
                     /locus_tag="AC16V7_01070"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HIR6255735.1"
                     /translation="MELVIPFQQVFSRRPTLQVRAPGRVNLLGEHVDYNDGMVLPVAL
                     DRAVYLVAAPTEDQIIHLRALDLSKAVSFRADQVEAKVDLEGEPLPGWARYPAGVAWA
                     LQEAGLEVVGAQVAYSSTVPIGAGLSSSAAVEVAFAGLWRAMGGWSVGNLDLARLCQR
                     AENHYVGVACGLMDQFASACGVEGHALYFDTRSLEWQSLPLPAGTVIIVADSGVRRSL
                     TSSAYNQRRQACEQAVELLRQYKPDMRALRDIAPPEFAAYSALLPPEIARRAEHVVRE
                     IARVESAVAALQRQDAQIFGALMYAGHASLRDFYEVSIPELDTLVDITRDLPGCYGAR
                     LTGAGFGGCTVNLVEESQAEAFIQSLSEAYQGRTGRQAQVYLCRASSGVEVKPVLT"
     gene            complement(1516..3045)
                     /locus_tag="AC16V7_01075"
     CDS             complement(1516..3045)
                     /locus_tag="AC16V7_01075"
                     /inference="COORDINATES: protein motif:HMM:NF014683.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015295 - solute:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HIR6255736.1"
                     /translation="MTFILALTPIVLILFLMVGLRWGAAQAGGAGYLVALLIAILFFG
                     AGPELIAYAHAKALLLAIDVLLVIWAAFLVYRVADEAGAIRTIGQALPQLTNDQGMQA
                     LVIGWIFASFLQGVGGFGVPVAVIAPILVSLGFTPLASVVIPSIGHGWAVTFGSLGSS
                     FLALMAATGLPSTALAPPSALFLGISGLGVGWMVAHATGNWKAVRRLFLPVLILGIVM
                     GIVQYLVAVSGLWNIAAFAGSIAGLAMAFPIARRYRGDQKDNGVLDGRALLVALSGYA
                     ILVLVILGVQLTPGIKSFLGQVVVQVQFPELHTALGFITPAGAGRKIAVFGHTGTLLM
                     YSAILAYVVYRLNGLYKPGSVRRILDGTLRRVMSSSVSIASMVAMAVIMENAGMTDRL
                     ARGLAEGVGVLFPAVAPWIGALGAFMTGSNTNSNVVFGALQLRTAELLGYTAAIILAG
                     QTSGAALASVMAPTKVVVGASTAGMAGREGDVMRKLVIYAVLLVLLISILTSIGVILS
                     I"
     gene            complement(3092..3862)
                     /locus_tag="AC16V7_01080"
     CDS             complement(3092..3862)
                     /locus_tag="AC16V7_01080"
                     /EC_number="2.8.3.-"
                     /EC_number="4.1.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004595063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase subunit beta"
                     /protein_id="HIR6255737.1"
                     /translation="MIEYSSSELLTINAARLLRDDDVVFVGVGLPNLACNLARHTHAP
                     SLVMIYEAGVIGAQPARLPLSIGDPTLVSGALSVCSMYDIFAFYLQRGNIDVGFLGGA
                     QIDRFGNINATVIGDYQHPKVRLPGSGGSMEISAWANRCYILTPHQKRRFPEKVDFRT
                     SAGFLGGRAEREAANLRGGGPQAVVTDLGIMEPDENGELTLTALHPGCTVEQAKANTG
                     WDLKVAAQLRLTDPPSEEELRILRLELDPDGIYLKSSA"
     gene            complement(3859..4296)
                     /locus_tag="AC16V7_01085"
     CDS             complement(3859..4296)
                     /locus_tag="AC16V7_01085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR6255738.1"
                     /translation="MDYRDLKIYQKPREVVQLLNKIMNSWPKTMQSQEIKRQLFRSAT
                     SIGANIAEGHGRHVGQEYIHYLTIAQGSANEVDHWLNTALDCGIGSAKELNNLISLNN
                     ETRKMLNASINTLKKRRDNKIMHETPAPYAPIPYSPENSEEKL"
     gene            complement(4329..5231)
                     /locus_tag="AC16V7_01090"
     CDS             complement(4329..5231)
                     /locus_tag="AC16V7_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HIR6255739.1"
                     /translation="MSKLLSLSEAVARYVQDGDLVYAAGFTHLIPFAAGHEIIRQKKR
                     DLVLARATPDLIYDQVVAAGCARKIIFSYMGNPGVGSLRICRAELEAGNLDWEEYSHF
                     SMISRIQAGATGLPFMPMKPTAAVDLERSNPNYRTVVDPYSGQNVVVVPALNPDVAIV
                     HVQRADAQGNAHIWGIIGEQKEAAFAADRVILTAEEIVDEEIIRSDPNRTLIPGFIVD
                     AVCHVPYCAHPSYTQGYYDRDNAFYLDWDRISQSKEAVQSYLDEWVYGVSDRAEYWEK
                     LGNEVHQRLQVGSRPSATVDYGKY"
     gene            complement(5237..5476)
                     /locus_tag="AC16V7_01095"
     CDS             complement(5237..5476)
                     /locus_tag="AC16V7_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255740.1"
                     /translation="MFKWQVGQFLVLLGLIGLVVFFMTDQVREPIYSFFCYGLVVLFF
                     GGYLMWIGRGPAPPPSGRFRLFKGRGDKKVDENKK"
     gene            complement(5634..>7100)
                     /locus_tag="AC16V7_01100"
     CDS             complement(5634..>7100)
                     /locus_tag="AC16V7_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR6255741.1"
                     /translation="EYAMLIDGKDRFAAEKFEDHSPINTDMVLGVFQKGTEQDAHDAL
                     AAARRAFPVWSKMKWQERVALLRKAADLIDERTFEIGAAMALEVGKNRMESLGDVAET
                     ADLVRYACYQMEKSEGFRVEMGRDPLAGYTATNFSVLRPYGVWQVISPFNFPTALTGG
                     PTGSALVTGNTVVMKPATDTPWVVRLLVECFRDAGLPDGVVNYVTGPGRTLGQALIES
                     PEVDGVTFTGSYDVGMKIFRDYAQGRYVRPIILELGGKNPAIVSRNADLERAAIGIMR
                     SAFGLQGQKCSACSRVFVEEPVYDKLVDRLVDLTKKLVVGDPTERQVYMGPVINQNSY
                     KDFKNYTEELSQAGTFLTGGKVVTDGDYAKGYFCEPTLVADVPLDHRLWQHEMFLPIT
                     TVARIGSLDEAMQHANSVTYGLTAGFYGSKEETQWFFENIQAGVTYANRPQGATTGAW
                     PGFQPFGGWKGSGSSGKNAGGHYYLQLYMHEQIQTMIE"
BASE COUNT         1754 a         2113 c         1748 g         1485 t
ORIGIN      
        1 gattgttcgc aatcgccctg atcaggaaat aaatctcatt cccctctatc gctgaatctc
       61 cccagcgatt ataagggtat ttttgcacaa cctgctatca cgtaaggaca ggttttactt
      121 caacccctga gctagcacgg cacaggtaga cttgcgcctg gcgacccgta cgcccctggt
      181 aggcctcgct gagggactgg atgaacgcct cagcttgact ctcctcaacc agattaaccg
      241 tgcaaccgcc gaacccggct ccggtcagcc gcgcaccgta gcaacctggt aaatcgcggg
      301 tgatatccac cagcgtgtct aactccggaa tactgacctc ataaaaatcg cgcaggctgg
      361 catgaccagc gtacatcagc gcgccgaaga tctgggcatc ttgccgctgc agggcagcta
      421 ccgccgactc gacccgggct atctccctta ctacgtgctc ggcgcggcgt gcaatctcag
      481 gaggtaggag agcgctgtaa gcagcaaact ctgggggagc aatgtctcgc aaggcgcgca
      541 tatccggctt atactggcgc aatagttcca ccgcctgctc gcaagcctgg cgccgctggt
      601 tataggccga gctggtcagg ctgcggcgca cgcccgaatc agcgacgatg atcactgtcc
      661 ccgctggcaa gggtagcgat tgccactcca gactgcgcgt gtcgaagtac agtgcatgcc
      721 cttccacccc acaggcgctt gcaaactgat ccatcaatcc acaggctacg cccacgtaat
      781 gattctcggc gcgctgacat aagcgggcca agtccaggtt gcctaccgac cagccaccca
      841 tcgcccgcca tagcccggca aaggccactt ccacggcagc tgatgagctc aagccagcac
      901 ctattggcac ggtcgaagag tatgccacct gggcaccaac cacctccagc ccggcttcct
      961 gcaatgccca ggctacaccc gccggatatc gcgcccagcc tggtagcggc tcaccctcca
     1021 gatccacttt ggcttccacc tgatcggccc ggaatgaaac cgctttgctc aaatccaaag
     1081 ctcgcaggtg gataatctgg tcttcggtcg gggcagcaac caaataaaca gcccggtcca
     1141 gagccaccgg cagcaccatt ccatcgttgt aatccacatg ctcgccgagc aggttcaccc
     1201 gccctggtgc acgcacttgt aatgtcgggc gacgggagaa tacttgttga aatgggataa
     1261 ccagttccat gatcttaatc ccaacgaact catgctgatg gagaaaactc ttctcgaaat
     1321 ctactcaact gaaagcctat ttgaccaaca taaccaacac attccctgat acgaacagcc
     1381 caattaatcc ccaaatccag atttccgaac ccgcgccagc ggcgaagtac aatcctgctg
     1441 aggataggac catcaaccca aaagcaaacg aatggatctc gtaggtttta ttttgcttcg
     1501 aatttttcat ctcacttaaa tactcaagat caccccaata gatgtaagga tgctgatcag
     1561 caaaaccagt agcactgcat aaatcaccag ttttcgcatg acatcaccct ctcttcctgc
     1621 cattcccgcc gtgcttgcgc ccacgaccac cttcgtcggt gccatcacgg aagccagcgc
     1681 tgcccctgat gtttgcccag ccaagattat ggcagcagtg tagccaagca gctctgctgt
     1741 gcgcaactgc aatgcgccaa ataccacgtt cgaattcgtg ttactacccg tcatgaaggc
     1801 tcccaaagca ccgatccaag gcgcaactgc tggaaagagg acacccaccc cttccgctaa
     1861 gccgcgcgcc aagcgatcag tcatccctgc gttttccatg atcactgcca tggccaccat
     1921 agaggcaata cttacactgg aagacatcac tcgccttaat gtgccgtcca gaatgcggcg
     1981 cactgatccg ggtttgtata agccatttaa tcgatacacc acataggcta agatcgccga
     2041 atacatcaac aacgtcccag tgtgcccaaa tacagcgatc ttacgccctg caccggctgg
     2101 ggtaataaag cctaacgcgg tgtgcaactc cgggaattga acctgcacga ccacctgccc
     2161 cagaaagctc ttgatccccg gggtaagctg tacacccaat atgaccaaaa ccagaatagc
     2221 atatcccgag agcgccacca agagagcacg cccatccaaa acaccattat ctttctggtc
     2281 gccgcgatac ctgcgggcaa ttgggaatgc catagctaac ccagctatcg aaccggcgaa
     2341 ggcggcgata ttccataaac cgctgactgc caccaggtat tggacgatgc ccattacgat
     2401 acctaaaatc agaacaggca agaacaagcg gcgcacggct ttccaattac cagtggcgtg
     2461 cgcgaccatc caacccacgc caagcccgga aatgcccaga aaaagggcgg aaggaggcgc
     2521 cagagctgtg cttggtaacc cagtagctgc cattaaagcc aggaaggaag aacccagaga
     2581 gccaaatgtc accgcccagc catgacctat cgaagggatg accacagaag ccagcggcgt
     2641 aaaacccagg ctaaccagaa tgggtgcgat caccgccacc ggcacgccaa atccgcctac
     2701 gccctgcaag aacgaggcga agatccagcc gatgaccagt gcctgcatgc cctggtcatt
     2761 ggttagctga ggcagtgcct gaccaatggt tcggattgca cccgcctcat cagcaacgcg
     2821 atacaccaag aatgctgccc agatgacgag caagacatct atcgccagca gcaaggcttt
     2881 cgcgtgggca taagctatta actcggggcc cgcgccaaaa aacaatatgg cgatcagtaa
     2941 ggccaccaga taacccgcac cgccagcctg cgccgctccc cagcgcaagc caaccatgag
     3001 gaagagaatg aggacaatag gtgtaagagc gaggatgaat gtcatgagga atacagggat
     3061 cgaggtccct tgtccaattc agttgatgtg tttatgcaga actcttaaga tagattccat
     3121 ctgggtcgag ttcaaggcgc aggatgcgca actcttcctc agagggagga tcggtaaggc
     3181 gcaattgtgc agcaactttc agatcccagc cagtatttgc tttcgcctgc tcgactgtac
     3241 aacccggatg caatgccgtc agggtcaact caccattttc atcgggctcc atgataccca
     3301 ggtcggtgac gacagcctga ggcccgccgc cgcgcaggtt ggcagcctcg cgctcggcac
     3361 gcccgcccaa gaaacctgcc gaagtgcgga aatccacttt ctctgggaag cggcgctttt
     3421 ggtgcggggt gaggatatag caacggttcg cccaagcaga aatctccatc gagccccctg
     3481 agccgggcag gcgcaccttg ggatgctggt agtctccgat cactgtggca ttgatattgc
     3541 caaagcggtc gatctgcgcc cctcccagaa aacccacatc gatattgccg cgctgcaaat
     3601 agaaggcaaa gatgtcatac atgctacaca ccgagagtgc cccactgacc agtgtaggat
     3661 cgccgatgga gagtggcagg cgcgcaggct gggcgccaat cacacccgct tcgtagatca
     3721 tcaccaggct gggtgcatgt gtatgtcggg caaggttgca ggccaggttg ggtagtccaa
     3781 cgcctacaaa gaccacatcg tcatcccgca aaagccgggc agcatttatg gttaataatt
     3841 cggaagatga gtactctatc ataatttctc ttcagagttt tcaggggaat aagggatagg
     3901 ggcataaggg gcaggtgttt catgcatgat tttgttatct cgacgttttt tgagtgtatt
     3961 gattgacgca ttgagcatct tacgcgtttc attattcaaa gaaattaagt tatttagttc
     4021 ttttgcagag cctatcccac agtccaatgc agtattgagc caatgatcca cctcattcgc
     4081 cgaaccttga gcaatcgtca aataatggat atattcctga cctacatgtc tgccatgccc
     4141 ttcagcgata ttagctccga tcgatgttgc cgatcgaaac aattgtcttt taatttcctg
     4201 ggattgcata gttttgggcc aagaattcat gatcttattc agtagctgga ccacctcgcg
     4261 aggtttctgg taaattttta agtctctata gtccacagcc ttctccctta tcccctatcc
     4321 cccctgcctt aatactttcc ataatccacc gtggcgctgg gtcgggagcc gacctgcaag
     4381 cgttgatgca cttcattccc aagcttctcc cagtattcgg cgcggtcact cactccatac
     4441 acccactcat ccagataact ctgtaccgcc tctttggatt gggagatgcg gtcccaatcc
     4501 agataaaaag cattatcacg gtcgtagtac ccctgggtgt aggagggatg tgcacaatac
     4561 ggcacatggc agaccgcgtc aacaataaag cctgggatca gcgtgcgatt tggatcggag
     4621 cggatgatct cctcatccac aatctcctct gcggtcaaga tcacgcgatc ggcagcaaaa
     4681 gcggcttcct tttgctcgcc aatgatcccc caaatatggg cattgccttg ggcatcggcg
     4741 cgctgcacat gaacaatcgc gacatccgga tttaaggcgg gcaccaccac cacgttctgt
     4801 ccggaatagg ggtcaaccac tgtacggtag ttgggattgg accgctccaa atccacagct
     4861 gcagtgggtt tcatgggcat aaaaggcaaa ccggtcgctc cagcctgtat gcgagagatc
     4921 atactgaaat gcgagtattc ttcccaatcc aggtttccgg cttcgagttc agcccggcag
     4981 atgcgcagcg atccgacgcc cgggttgccc atgtaggaaa agatgatctt tcgggcgcaa
     5041 cccgccgcta ccacctggtc gtaaatcaag tctggcgtgg cgcgcgccag cacaagatcc
     5101 cgctttttct ggcggatgat ctcatgccca gccgcaaatg ggatcaggtg ggtaaagcca
     5161 gctgcgtata ccaggtcacc gtcttgaaca tagcgggcaa ccgcttcgga taaagagagc
     5221 aatttggaca taagcctcac tttttattct catcaacttt tttgtcgcct cttcccttaa
     5281 agaggcgaaa acggccgctg ggcggcgggg caggacctcg tcctatccac atcaaatagc
     5341 ctccaaaaaa gagcaccacc aagccataac agaagaacga ataaatgggc tccctgacct
     5401 gatcagtcat aaaaaagaca accaggccga tcaaacccaa taagaccaga aactgcccca
     5461 cctgccactt aaacatgatt attcacccct tcgctggaag agagggcgga aaacaaatag
     5521 cgcaattgga gtagtgcggg cgcagagcgt ccgtaaaacc ccaattgcgc ttattctccc
     5581 ctcctccttg tcatccgagg aaggggcaag ggggaaataa aactcaatcc aactcactca
     5641 atcatcgtct ggatttgctc atgcatatag agttgcagat aataatgccc accagcattc
     5701 ttgccactgg atccggatcc tttccaccca ccaaagggct ggaagccagg ccaggcaccg
     5761 gtagtggctc cttgcggtcg attggcataa gtcacaccag cctgaatatt ttcaaagaac
     5821 cactgggtct cttcttttga gccataaaaa ccagccgtca atccataggt gacgctgttc
     5881 gcgtgctgca tggcttcgtc caggcttcca attctcgcca cagtagtgat gggcaagaac
     5941 atctcgtgct gccacaggcg gtgatccagg gggacatcgg ctacaagggt tggctcgcag
     6001 aaataaccct tggcataatc gccatcggtg accaccttac ctccggtaag gaaggtaccc
     6061 gcctgagaga gttcctcggt atagtttttg aaatccttgt aggaattctg gttgattact
     6121 ggccccatat aaacctgtcg ttccgtggga tcgcccacca ccagcttctt ggttaggtcc
     6181 accaggcgat ccaccaactt gtcatacaca ggttcctcga cgaatacgcg cgagcaagct
     6241 gagcattttt gaccctgcaa gccaaaggct gagcgcatga tgccaattgc ggcgcgctcc
     6301 aggtctgcat tgcgagagac tatggcgggg tttttgccac ctagctctaa gataatcggg
     6361 cgcacgtaac gcccctgggc gtagtcgcgg aatatcttca tgcccacatc gtaagaaccc
     6421 gtaaaggtga cgccatccac ctctgggctt tcgatgagtg cctgaccaag agtgcggcct
     6481 ggaccagtga cgtaattcac cacgccatcc ggcaggccag catcacggaa gcattcaacc
     6541 agcaggcgca ccacccaggg tgtatcggtg gcgggcttca tcacgacggt atttcccgtc
     6601 accaaggcag acccagttgg cccacctgtt agcgcagtgg ggaagttgaa ggggctgatc
     6661 acctgccaca ccccgtaggg tcgcaaaaca gagaaatttg tggcagtgta acctgcgagc
     6721 ggatcgcggc ccatctccac ccggaagccc tcgctcttct ccatttgata acaggcatag
     6781 cgcaccaagt cggcagtctc tgccacatcg ccaagagact ccatgcggtt cttgcctact
     6841 tccaaagcca tcgccgcgcc gatctcgaaa gtgcgttcgt cgatcaaatc cgcggctttg
     6901 cgcagcaggg caacacgctc ctgccatttc atcttgctcc acacggggaa ggctctgcga
     6961 gccgccgcaa gcgcatcgtg ggcgtcttgc tcggtccctt tctggaatac gcccagcacc
     7021 atgtctgtat tgatcggcga gtgatcttcg aacttctcgg cggcaaatcg gtccttccca
     7081 tcaatgagca tagcatattc
//
LOCUS       DBNHOH010000064         1334 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1028363, whole genome shotgun sequence.
ACCESSION   DBNHOH010000064 DBNHOH010000000
VERSION     DBNHOH010000064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1028363"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="AC16V7_01105"
     CDS             complement(<1..299)
                     /locus_tag="AC16V7_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255742.1"
                     /translation="MAQISQAQMRGRAYPRMNIVRLGLGIALSALSGVMLLLAFPPYG
                     LWPLAWFALVPGLLAQYRLLPAKWTSLASAVYSGVWLGPFLARLFGPGYGPIFQY"
     gene            750..>1334
                     /locus_tag="AC16V7_01110"
     CDS             750..>1334
                     /locus_tag="AC16V7_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255743.1"
                     /translation="MKCPNCNHISETALMQCSQCGEAYDRHSLETFQHLEYLLSWLDS
                     RAETLDAATLKNLRADAMAESQRLLDELLPAPEPAAVIEPSVEEISLSPDAERELALI
                     LATRALIPGWIEAKLIGGRTAGELGDHLTRQAQAIHAQAGIIELELPEPSEADVHTFA
                     MGALDGWIEQKLFTASDASLLRDCLRENLGLTLQP"
BASE COUNT          335 a          378 c          334 g          287 t
ORIGIN      
        1 aagtattgga agattggccc ataaccagga ccaaagagcc gcgccaggaa tggacccagc
       61 catacgccgg agtagaccgc tgacgccagg ctggtccatt tggctggcaa gagcctgtat
      121 tgagccaaca agccaggcac aagcgcaaac caagcaagcg gccacagtcc atagggcggg
      181 aaagccagca gcagcatcac gccgctcagg gcagacaggg caatccccag ccccaagcgc
      241 actatattca tcctgggata tgccctcccc cgcatttgtg cctggctgat ctgagccata
      301 acaacctccc ctccacctgg tattacttta caatatttgg tagccagagt caacttgagc
      361 gcgcccaggc tcgggtaaaa gcatggatga atttctaacc aaaagtcatc gacatccttt
      421 tgtccgcacc cccgttttat ttgtgaaaaa aattattaac ttgatatttg gtgaaaattt
      481 atataaaatc taattgaaat tctccacaat cggatggcat gttgaagtag attttcactt
      541 tccctcacta cacttaacgc ggcactccaa tgattgcaag cagtcagaat cgacttgatc
      601 atcgccccgc atgacccttc gcttaaaagg gacaccagcg gggctcgttt tttttctggg
      661 gtggcttttt tagacggttg tgaactttca cagagcttgt aagctgatcg accacgaagc
      721 acgacactcc gttcattgga ggccaagtca tgaaatgtcc caactgcaat cacatctctg
      781 aaactgcgct gatgcagtgc agccagtgcg gcgaagccta tgaccgccat tctctggaga
      841 ccttccagca cctggaatac ttgctctcct ggctggactc gcgcgccgag acactagatg
      901 cggccaccct gaaaaacctg cgggctgacg ccatggctga gagccagcgc ttgctggatg
      961 agcttctccc agccccggaa cccgcagccg tcatcgaacc tagtgttgaa gagatctcgc
     1021 tcagccctga cgcagagcgt gaattggcgc tgatcctggc tacccgcgcc ttgatccctg
     1081 gttggatcga agccaaactt atcggaggta ggacagctgg cgaactaggc gatcacctga
     1141 ccaggcaagc ccaggcgatc cacgcccagg caggtatcat cgagttggaa ctacccgagc
     1201 cctcggaagc tgacgtacat actttcgcca tgggtgcgct ggatggctgg atagagcaaa
     1261 agctatttac cgctagcgat gcaagcctgt tgagggactg tctgagagaa aacctggggt
     1321 tgaccttaca acct
//
LOCUS       DBNHOH010000065         1929 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_131150, whole genome shotgun sequence.
ACCESSION   DBNHOH010000065 DBNHOH010000000
VERSION     DBNHOH010000065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_131150"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..1033)
                     /locus_tag="AC16V7_01115"
     CDS             complement(41..1033)
                     /locus_tag="AC16V7_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6255744.1"
                     /translation="MKILIASSIDSDAIEQLKQHHKVILAFNKPNEPLETLIPDCEVL
                     IFRSGVKISAQLMERSPNLKLLIRAGSGLDNLDLDYVQRNGLVLIRIPQPGAKAVAEM
                     TFALMLALSRRLFEADQSMRQARWKKYELSGYLLQGKTLGIIGIGEIGGRVAEMGMAW
                     GMKAISHDGFLSPERARLLSEKGVELLDFDQVVSQSDYLSLHVPLNESTRYMINASVL
                     ENMKPGSYLINLARGGIVDEQALYKALTSGGRLRGAALDVHEKEGEGKLSPLASLNNV
                     ILTPHIGAMAVDAQREIGQRVVEIVKSYTASPNQVKVPRNGGVVIESDSQTVRI"
     gene            complement(1261..>1929)
                     /locus_tag="AC16V7_01120"
     CDS             complement(1261..>1929)
                     /locus_tag="AC16V7_01120"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HIR6255745.1"
                     /translation="PSAQIIHIIRDGRDVALSMLRSYRGSKFFYIDVCYAAHSWKRRV
                     RQARSYGKTLGIDRYHEIRYEELVANPTEVLSAICNFLGEAYEPSMAEPYKEAKAHFH
                     SKGIHAATRQPLTTKSVGRWQSEMSEADQRLFQAIASDLMNELGYQTTELGKMSLAEK
                     ARFAQLHAKYYMVDVSRRTLQASGIFHPTSVIRSIKLPYPINRYRSSKKKMGEILKKP
                     DSLF"
BASE COUNT          473 a          467 c          448 g          541 t
ORIGIN      
        1 ttcaatagct gtttaaaatg ggtcgttttc ctggtcatgt tcaaatcctc actgtttgag
       61 aatcgctttc aataaccacc cccccatttc gcgggacttt tacttggttt gggcttgctg
      121 tgtaggattt gacaatctca accacccgct gcccaatctc acgttgcgca tcgactgcca
      181 tggcgccaat atgaggggtg aggatcacat tgttcaggct agccaggggt gataatttgc
      241 cttcgccttc tttctcatgg acatccaggg ctgcccccct caatctgcct ccgctggtca
      301 atgccttata caatgcctgt tcatcgacga tgccaccgcg ggctagattg attagataag
      361 agccgggctt catgttctca aggactgagg cattgatcat atatcttgtg gattcattga
      421 gcggcacatg caggctgaga tagtccgatt gagatacgac ttggtcaaaa tccaacaact
      481 cgacaccctt ctcgctgagc aggcgcgccc gttctgggga gaggaatcca tcatggctga
      541 tcgctttcat gccccaggcc atccccatct cagcgacgcg gccgccaatt tctccaattc
      601 caataattcc cagtgtttta ccttgtaaaa ggtacccaga tagctcgtat tttttccagc
      661 gagcctgtct catggattga tcggcttcga aaagcctgcg actgagcgcc agcatcaagg
      721 caaacgtcat ctctgcaacc gccttagccc caggctgtgg tatgcggatc agcaccaatc
      781 cattccgctg cacataatca aggtccagat tgtccagtcc agatcctgcg cgaatgagca
      841 gcttaagatt gggtgatctt tccataagtt gtgcactaat ttttacacca ctgcggaaga
      901 tcaaaacttc gcagtcaggg ataagcgttt cgagtggctc gttcggtttg ttaaaagcta
      961 agatgacttt atggtgttgt ttaagttgtt caatggcgtc actatcaata gagctagcga
     1021 taagaatttt catgatgtga tcatctccga taagcgctta ggtatcaata tgaacgaaat
     1081 cttgctcagc ttgttactag ccgctggcag gctttcaaga ttgatggatg tgtaaatatc
     1141 taacggaagt ttggtttagg gcagcgtagg caatttgatg gtggttatcc aacactcagt
     1201 taaaggatga gagcttgttc cttaacccct atatggatta tcccagcaag agagcataga
     1261 ttagaaaagg gaatcaggct ttttcaagat ttcgcccatt ttttttttgg atgaacgata
     1321 tctatttatc ggatagggca attttataga tcttatcact gaggtggggt ggaaaatacc
     1381 agatgcttgt agcgtgcgtc ggcttacatc caccatgtag tacttagcat gcagctgtgc
     1441 gaaacgggct ttctcagcta agctcatctt tcccaactca gtagtttggt agcctaactc
     1501 gttcatcaga tcgctggcta ttgcctggaa taagcgctgg tctgcctcag acatctcact
     1561 ctgccagcgc cccacgcttt tagtagttaa aggttgtcgc gtagccgcgt gaattccctt
     1621 tgaatgaaag tgcgctttcg cttctttgta aggctcagcc atagacggct cgtatgcctc
     1681 acccagaaaa ttacagatcg ctgacaacac ctcagtcggg ttggcaacta attcttcata
     1741 gcgaatttcg tgatagcgat caattcccaa ggttttgcca taagaccttg cttgtcggac
     1801 gcgcctcttc caactatgag cagcgtagca cacatcaatg taaaagaatt ttgagcctcg
     1861 atacgagcgt agcattgaaa gagcaacatc acgcccatcg cgaatgatgt gaataatttg
     1921 ggcagaggg
//
LOCUS       DBNHOH010000066         2571 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1348727, whole genome shotgun sequence.
ACCESSION   DBNHOH010000066 DBNHOH010000000
VERSION     DBNHOH010000066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1348727"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..1575)
                     /locus_tag="AC16V7_01125"
     CDS             complement(22..1575)
                     /locus_tag="AC16V7_01125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015158548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR6255746.1"
                     /translation="MSDSIIIIGAGIAGLSAGCYGQMNGYRTQIFELHDIPGGLCTAW
                     ERKGYIFEGCIHYLFGSGQDQPFNHIWQELGAVQGRQMIHHDEFIRIKDNDGKTLIVY
                     ADPDRLEEHLLELSPADRDLIKDFTKGVRQFTSFDMSVLQEKPRDLFGPTDGLNLGRR
                     MLPFIQPLMKWGNISAQDFANRFRDPFLRRAIPQMFAWPEIPVMAGLSLLAYMHTKNA
                     GFPIGGSLPFAQTVEQRYIELGGKVHYKAQVEKILTENGRAVGVRLYDDSVHYADTVI
                     SGADGRGTVFDLLDEDFTDAKINKTYDGHLPIISQVQVSLGVSRDLSAEPHWVVYLLD
                     EPVIIAGEERHELSVKHYCFDPSLAPIGKSVIEVMLPSQYGYWQRIYGRKLYDTEQLQ
                     VADQVIELLENIYPDLSQSIEVTDVATPLSYERYTGNWQGSTCGWLLTKETMRMMILG
                     IKKTLPGLDRFYQVGQWVEPGGSLPLAAMSGRNAIQLICHRDGKPFVTSLPASQAPRT
                     DFRLGSERK"
     gene            1882..>2571
                     /locus_tag="AC16V7_01130"
     CDS             1882..>2571
                     /locus_tag="AC16V7_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255747.1"
                     /translation="MAKITHKLNNSLEGALAGGGDPATSPLYVFGPFLRMIVVAGVAQ
                     ITFGASIWLVVLTIAVVSAMYRLVMLWVTDGSGGSGLTEEEFGSWAAKINAAITFVEY
                     TLTFLVSMAAMVTFIADRIPILNNKFLFLQYRTLIAILLSFLTGWLVNRGPKVAARTF
                     GPATGGVLLLLWAMVISSIFKYGLRLPTLSLQAFSLQNLSYTIRGYASILAVMTGIEV
                     FANLVAAYEGSP"
BASE COUNT          624 a          625 c          655 g          667 t
ORIGIN      
        1 tccccctcgg tttggctgac ctcacttccg ctccgagccg agacgaaaat ctgtcctcgg
       61 ggcttggctg gcgggcaggc tggtcacgaa aggtttacca tcgcgatggc agatcaactg
      121 gatggcattg cgacctgaca ttgcggccag cggcaggctg ccacccggct caacccattg
      181 acccacttga taaaaacgat ccaggccggg caaggtcttt ttgatgccca ggatcatcat
      241 gcgcatagtc tctttggtca gcaaccagcc acaggtcgag ccttgccagt tgccggtgta
      301 gcgttcgtaa ctcaagggag tggccacgtc ggtgacctca attgactgac tgaggtctgg
      361 atagatattc tccaataact cgatgacctg gtcagcgacc tggagttgct cggtatcgta
      421 cagctttcgc ccgtaaatac gctgccagta gccatactgg gagggtaaca tcacttcgat
      481 gacggatttc cctataggag ctaagctggg gtcaaagcaa tagtgcttca cactcaactc
      541 gtggcgttct tctccagcaa taatcaccgg ctcgtccagc aggtaaacca cccagtgagg
      601 ttcagcagat agatcgcgag atacccctag tgaaacctgg acctgggaga ttatcggcag
      661 atgaccgtca taggttttgt tgattttagc gtcggtaaaa tcttcatcta aaaggtcgaa
      721 aacggtgcca cgcccatctg cacctgatat gaccgtgtct gcgtaatgga ctgaatcatc
      781 gtataagcgc acacctacgg cgcgcccgtt ttcggtaagg atcttttcca cctgggcttt
      841 gtaatgaacc tttccaccca gctctatata gcgttgctcg accgtctgtg caaacggcaa
      901 tgatccacca atgggaaagc cggcattttt tgtatgcata taagccagga gcgaaagacc
      961 agccatcaca ggaatttctg gccaggcaaa catttgagga attgccctgc gaaggaaggg
     1021 gtcacgaaag cgattggcaa aatcttgggc agagatattg ccccacttca tcaacggctg
     1081 gataaatggc agcatgcggc ggcctaaatt gaggccatcc gttggaccaa aaagatcgcg
     1141 aggcttttct tgcaaaacag acatatcgaa ggaggtaaat tgccgcaccc ccttggtgaa
     1201 atccttgatc aggtcacgat ctgcaggcga tagctccaac agatgctctt ccaggcgatc
     1261 cgggtcggcg taaacaatca aagtttttcc atcgttatcc ttgatacgta taaactcgtc
     1321 gtggtgaatc atctgtcgac cttgaacggc acccagctct tgccagatat ggttaaatgg
     1381 ttgatcttgc ccggatccaa acaggtagtg aatgcatcct tcgaagatat aacccttacg
     1441 ttcccaggcg gtgcataatc ctccgggtat atcgtgcagc tcgaagatct gcgtccggta
     1501 accgttcatc tgaccgtagc acccagcgga tagaccagca attcccgccc caatgataat
     1561 gatcgaatcg gacatgtaag accctcccat gcgtgtcggt gtagcatatt tcggcaggat
     1621 tattgtacta cgttgctttt aagcttggca ggagtaaagc aaaaaaactt gtacggggag
     1681 gggtttgtgc agtatcctgc ctatcgatga ccttttcgaa tggataaatc ccgttgttca
     1741 cctggagagt gggtttaccc gatttagaag aatgtgacta tcggaaggga gcgatatttg
     1801 ggtaaaatta gatgcacgct gtgttgcgtt gaatttaatt catctgtaca ctggagacaa
     1861 ttatggatcg ggaaatcatc aatggctaaa ataactcaca agcttaataa ttctttggag
     1921 ggagcgctgg ccggaggcgg cgacccggca acttcaccgt tatacgtttt tggccctttc
     1981 ctgcgcatga ttgtggtcgc tggagtagcc caaatcacat ttggagcctc tatttggttg
     2041 gtggtgctca ccattgcagt ggtttccgcc atgtaccgct tggtgatgct ttgggtgact
     2101 gatggaagtg gaggaagtgg tttaaccgag gaagaatttg gaagctgggc ggctaagata
     2161 aatgccgcaa ttacttttgt tgagtataca cttacttttc tggtcagcat ggctgccatg
     2221 gtcactttta tcgcagaccg gatacccatt ttaaataata aattcctgtt cttacagtac
     2281 cgtactctca tcgcaatttt gctcagtttc ctgactggct ggttggtaaa ccgtggccct
     2341 aaagtagcgg cgcgcacgtt tggtcctgca actggagggg ttctgttact cctttgggca
     2401 atggtgattt cgagcatctt caaatatgga cttcgtctcc caaccttgag cctgcaagca
     2461 ttttctttac agaacttatc ctacaccatt cggggatatg ccagtatcct ggcagtgatg
     2521 acagggattg aagtatttgc caatttggta gcagcttatg aaggcagtcc c
//
LOCUS       DBNHOH010000067        16471 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1028489, whole genome shotgun sequence.
ACCESSION   DBNHOH010000067 DBNHOH010000000
VERSION     DBNHOH010000067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 16471)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16471)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16471
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1028489"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..2000)
                     /locus_tag="AC16V7_01135"
     CDS             complement(216..2000)
                     /locus_tag="AC16V7_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR6255748.1"
                     /translation="MTPEIALTLAIVLGALVLFGTDKVRVDLVALLVLLTIGLTGLVS
                     PKEVFAGFSNSAVITVWAVYIVSGGLFNTGVADTLGKFILRLAGKNEMRLIAVIMLTC
                     GILSAFMNNVGATAMLLPAVVSISRQNKISVSKLLIPLSFSSLLGGKLTLIGTPANIL
                     ATSIVAENGLPTFSFFDFTSLGIVVFVTGILYMVFIGRHLLPIRQSPSEQQIPQQLKA
                     YVSEVRVLPKGKLVGHTLLESRLGADYDLTVIAVVREEKTLAAVDRNTVIQPDDILLI
                     EGSVESLMRATTDIGLDLTSHPHMDLDNIDNSNLEIFEAILAHYSTMVGQNLRELQFR
                     ERYGFTVLAIRRHGELITKRLRDISLKFGDDFLLQGTRDRLPVLQEGNDFLTLEPIKI
                     PQDHRKRMPIAIGLMLLVIGLVLFAKLDIAIAMVIGAVGMVLTRCLTMDEAYQNIDWR
                     TVFLVAGMLPLGMAMETSGTARYIADLFLGMTGGLGPLAALAGMYILAALVTQPMSNA
                     AAMVLVVPIALDTALELGANHLTFTLAVVIGAATSFLTPVGHKANVLVFGPGGYKFFD
                     YTRVGFLLTIFLFIATMIALPVLFPLFP"
     gene            complement(2017..4362)
                     /locus_tag="AC16V7_01140"
     CDS             complement(2017..4362)
                     /locus_tag="AC16V7_01140"
                     /inference="COORDINATES: protein motif:HMM:NF015557.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR6255749.1"
                     /translation="MTVEIILVLAILAIAILLFVTERVRMDVVAFLVLGGLAVAGLVT
                     PAQALSGFSNPAVVTVWAILILSGGLARTGVASLIGRYMLRLAGDSEVRLLLFLSITA
                     GILSGFINTVGVVSLFLPVVIDIARRTKRPPSKILMPLAFASLLGGLNTLIGTPPNIL
                     ISEALREAGLRPFQMFDYTPVGLSALLAGTVYMILVGRHLLPNLDLARDFSAERARDF
                     KSLYDLHEHMVILHMPEGSILNGKTLAESRLGSALGLNVVAIIRQGQIMRSPQPSFAL
                     KSGDRLLVEGRLEQFSELRGKNHIILDQEGFSVERLVSADVQLVEARLSPSSSLIGQT
                     LRDIGFRHSYAVIVLAILREGVPFRTNLENIPLLGDDILLLQGRRQHIEELKHDTDFA
                     ISTPSSLQDFKLEERMMVVQVPPNSSLVGKSLVESHLGDSYGLGVLGIMRSGETNLMP
                     SPDERLQENDVLIVKGRYSDLHLVEGLQSLEVENGPPPDLGELESEEIGLVEAVLSPH
                     STLAGKTLRELNFRFKYGLTVLAIWREGRAYRTNLRDTELRFGDALLLYGPRERLRML
                     GSEPDFLVLTEEAQEPPRREKIPAALFIMALVLIPVVFGWLPIAISAILGVALMILTN
                     CLTMEEAYRYIEWKAVFLIAGLLPMGIAMEQTGAAEILANGMVTLIGNMGPMVLMAGF
                     FVVAALASQVMPNPAVAVLLAPIALRTASDLGVSPYPLMMTVAVSASAAFLSPVGHAA
                     NLLVMGPGGYRFADYIKVGFLLTIVTMIVAILVIPILWPFS"
     gene            4723..5514
                     /locus_tag="AC16V7_01145"
     CDS             4723..5514
                     /locus_tag="AC16V7_01145"
                     /inference="COORDINATES: protein motif:HMM:NF013078.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HIR6255750.1"
                     /translation="MQLAYNLSVPTPFYHLSVAEDLLGQPRLGKQARLLLEAQRSAFL
                     FGNTAPDVQVISHQPRQDTHFFDLPLRSLAPSPWELLLRDYPSLARADLLPPAQAAFL
                     AGYLCHLQADWLWVKDIFVPVFGLHSTWGSFPQRLYLHNVLRAYLDRHILPSLANGAC
                     TSLAEARPAGWLPFVHERYLYEWRDFLVTQLQPQASVQTVEVFAARQGISPSEFYHLL
                     DSEEAMEREVFARIPRQALENYREILIAENAKLIHNYIARATAIP"
     gene            5543..6373
                     /locus_tag="AC16V7_01150"
     CDS             5543..6373
                     /locus_tag="AC16V7_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255751.1"
                     /translation="MKVIDNPEFREKPGTPAKLDSVLDSLGMGRSGSPELQSQEDLIA
                     SLEKTLDNRFVLLRNVTLEDLDTPIPLVLLGPSGVSVIFPSPARGVFRAKEETLEYMD
                     EYNRVYRQANPNLLARTIMMAKSAEAYLNSNFPAPIEVEPVLYFSDPGTHVESVRPSL
                     RILLVDGLERFIAGQLQAAVLYEREEVQKLVDLLLKASRITERQVSPYPERDAFSFVD
                     ESGLDKPSIADRLPRGEGIVSALNKIPFSGRQWLLLGCMVVVNVVVLIAFVLLVLFTS
                     "
     gene            6390..7121
                     /locus_tag="AC16V7_01155"
     CDS             6390..7121
                     /locus_tag="AC16V7_01155"
                     /EC_number="2.4.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004581 - dolichyl-phosphate
                     beta-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HIR6255752.1"
                     /translation="MTGPFLSIVIPAYNEEKRLPKTLEQVRSFLESQPYTAEVLVVEN
                     GSQDRTFEVAQDFARRHPGVSVLQETGRGKGRAVRRGMLAARGEYRFMCDADLSMPVE
                     EINYFLPPALQDFDIAIGSREAPGAIRYNEPHYRHLGGRAVNTMIRLLALPGLQDTQC
                     GFKCFRAPIAEELYRLQTLTGWSFDIELLYIARQRGYRIVEIPVQWYFNPESKLNVVQ
                     DAIKMGLDIFTIHLNNMKGVYDAKV"
     gene            7105..7800
                     /locus_tag="AC16V7_01160"
     CDS             7105..7800
                     /locus_tag="AC16V7_01160"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR6255753.1"
                     /translation="MTPRFDVSLLPPAPNFDFEIALWERGCCYVAGVDEAGRGALAGP
                     VAAGVVMFPDDPGLLPELVGVRDSKQMTHSERAMWAERLKQLALTWAVGFASVEEIDS
                     LGIVPATRLAARRATRALGEQPEHLLVDFLELPELPIPQTPLVKGDRRSLSIAAASIL
                     AKTERDGLLCELEARYPGYGLAQHKGYATQAHRDALRRLGPAPIHRHSFSPVSSLSPL
                     APLPGGSGEKTKT"
     gene            7846..9744
                     /locus_tag="AC16V7_01165"
     CDS             7846..9744
                     /locus_tag="AC16V7_01165"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR6255754.1"
                     /translation="MVRAKTRRAYGFWDSPLTPKRMAGGLHLNDARWDESGALVWLEG
                     RSERAALVVQAPGGGAPRDLNSEYSVRAKVGYGGGDFGVGHGQVYFVESTSGRIYRQS
                     LEYGLAQPVTPAFGHAAAPTPSPDGDWLLFVHTYEGQDSLGIAGTQGRFWPGRLVSGD
                     DFYMQPTWHPDGKQVAWIAWNHPNMPWDGTTLWLGNLTFPPNGLPLMGEAKPLAGDEN
                     TAIFQPEFSPDGRTLAYVSDASGWWQIYLYDLESGATRQLTDEQAEHGAPAWVQGMRT
                     YAFGPGGVSLYFIRNQAGFASLWKADVASGRLSKLELPAQYTWLEKIAVSPVDGQIAL
                     IASGGDTPPRLIVQHADGGTHLLRHSQAEDVPVSAYTLPQAITWQGMDDGTIHGLYYP
                     PHSETFEGIGLPPLMVLIHGGPTSQRMASFDEEVQFFTSRGYAVLQVNYRGSTGYGRL
                     YRNMLRGNWGIYDVQDAVSGARYLVEQGRVEGSRMVIMGGSAGGYTVLLALEQFPGFF
                     KAAVNLYGVTNQFDFVAGGTHKFEERYNDTLLGPYPEAGQVYRERSPIFFVDKIQDPV
                     AVFQGEDDNVVPRRHSDELVKSLQRRGVPHIYHVYPGEGHGFSKPETIEHLYQELDKF
                     LKQYVLLT"
     gene            complement(9888..10484)
                     /locus_tag="AC16V7_01170"
     CDS             complement(9888..10484)
                     /locus_tag="AC16V7_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255755.1"
                     /translation="MKARQAGSRHLLLLYRRWMDRLWRSTLILGLLLFGIWFWTWWYG
                     TSLLHGQDDLWLLLGAVVLLGFTLFAFFARGMAYVQPRRDHLRLVTPFLRTNISYRRL
                     VSTHPADFTQIFPIKNASWAQRRLLEPFYGMTAVVVELNNYPLPPVLLRLFLAPQMFY
                     RRAKGFVLLVPEWMALSTELDSLVGAWQQEQKRRQLVR"
     gene            complement(10602..11273)
                     /locus_tag="AC16V7_01175"
     CDS             complement(10602..11273)
                     /locus_tag="AC16V7_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HIR6255756.1"
                     /translation="MDQASRALAVHQRLIKRYGHPQWRNHLPPLDELVSTILSQNTND
                     LNRDRAFEALRRQFPSWEQVRDAPTDEIIQAIRPAGLANQKGPRIQQVLREITLQRGS
                     LSLDFLNDLSPAEASAWLMSFKGVGPKTAAIVLLFSLGKPAFPVDTHIYRVSGRIGLR
                     AAKMNAEQAHEHLAELLPPETYYAAHLNLIRLGRETCQARRPNCPTCPIQDLCDYYAQ
                     LSSAA"
     gene            complement(11276..12331)
                     /locus_tag="AC16V7_01180"
     CDS             complement(11276..12331)
                     /locus_tag="AC16V7_01180"
                     /inference="COORDINATES: protein motif:HMM:NF017864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HIR6255757.1"
                     /translation="MHAAHYLPLLELTRGSIVESIHFGAIAVVDARGNLIASYGDPQA
                     VSYLRSTAKPFQALPFIENDGHKFYKLSQREIAVMCASHSGTNEQVAVVQGLQKKTGV
                     SEADLQCGVHPLSHEPTLEAMRQRSEQVTPNRNNCSGKHTGMLAYARMKALPTEDYLN
                     PHHPIQQDILNTFAAMCKVTVEQIEIGTDGCSAPNFAVPLFNAALGLGRLCDPETGGI
                     LPPSRQVACHTILAAMTSHPEMIGGPDSFDTRLMQATGGRILAKSGAEGYFGLGLRPG
                     TLSPNSPGAGIAIKVSDGDARQRARPAVVLEVLHQLGVLESSEMDRLSEYGPSTGVYN
                     WQRLEVGRLRPGFQLSR"
     gene            complement(12347..13222)
                     /locus_tag="AC16V7_01185"
     CDS             complement(12347..13222)
                     /locus_tag="AC16V7_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6255758.1"
                     /translation="MHQLKPGLFYEDAYLGVTLGALIFSQGTIMIDAPLRTEDTRAWR
                     SALITLRGGSSRLLISLDAHPDRTLGTRAMECTVISHHKTAQVFRNRPVIFKGQSAEG
                     GADWETYDDVIGTRWISPDITFSEKMILYWGGPEVILEHHPGPASGSIWVVIPQAGVL
                     FVGDAVTPNQPPFLSQADLPIWIETLDHLENTFKGYTIVSGRGGLVEPDAVRAQQRYL
                     KNIYKGLERLAKRNALPEATENLINSLLKEVDYPDKFSDQYTQRLRSGLFQYYIHHYR
                     QETLPDQLDFEDSDS"
     gene            complement(13334..15418)
                     /locus_tag="AC16V7_01190"
     CDS             complement(13334..15418)
                     /locus_tag="AC16V7_01190"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HIR6255759.1"
                     /translation="MDKKAQDQSMLASLELLYDVSREIAAALDLRTVLHRVLFLSMKN
                     VRAISGSIIVLDGNQQPVESAIITGSQLHEQTTQQLRITYERGLAGWVIRNQKAVLVP
                     DTSQDERWLRRPDDDAERTGPKSAVSAPILARDHLVGVMTLVHPTPGFFTQDHLALVR
                     AITDQAGVAVLNARLYAESQRQTRAMTALAESAATITASLKTDDVLQQILEQISQALQ
                     VEVVSLALIDPQTQELVFRAASGEKGPNVIGIRLAPGEGIAGWVAREGIGIIVPDVRQ
                     DPRFLPTVDKLLNFVTKAIACAPIRLRGEVIGILQAINPKDGAFDPDALQVITGIGGL
                     AGTAIHHAQLFERMEAAHQRYRELFEDSIDPILITDLEGRILEANRQVAMITGMNRES
                     LLNAMIDHFQIVDQAELGEKFEKLASGETTSYETCLKAEGGREVPIQVYVRNVFIDGI
                     SNLQWILRDVTERKNLDNLRNDLISMIYHDLRSPLSNIVSSLDVIQALMPQDSDPAVE
                     PLLDIAIRATERIERLTNSLLDIRRLEAGQSIASRELVSPTSITKEAVKNVLPIVENK
                     NQEITLLIEENLPRVWVDTDMIGRVLTNLLENAVKHTPAGSTIYLGARLEGNQVIMWV
                     QDNGPGIPPDERERIFDKFSRLHGRGGPKGLGLGLAFCRLAVEAHGGQIWVDDGPEGG
                     SNFKFTLPTQMD"
     gene            15595..16221
                     /locus_tag="AC16V7_01195"
     CDS             15595..16221
                     /locus_tag="AC16V7_01195"
                     /inference="COORDINATES: protein motif:HMM:NF025605.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6255760.1"
                     /translation="MSPNTNPEREPGDTRQRIIQAAIQLFGKQGYSQTSTRAIAEAAG
                     VNEVTLFRQFGRKKDLLIACIEAHNAAGFSATFESELTGDYAEDILRMAELQIRDTTA
                     NLEILRLLSCDAHNMPELRQAMLAGGRGNLARLSRYFQRQIDRGVVRADLPAEVLASA
                     FDSLFSSNIVFEYLFQGNLSPRLTTNEVIRPLAALFVRGTQAIDQRSV"
     gene            16223..>16471
                     /locus_tag="AC16V7_01200"
     CDS             16223..>16471
                     /locus_tag="AC16V7_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6255761.1"
                     /translation="MLRILTIVLAVLTGIHGLIHLMGFAAYWPLAKINELPYKTSLLA
                     GRLEVGMAGMRVFSFLWLLATLGFVIAAIALAFGKSNWA"
BASE COUNT         3694 a         4322 c         4828 g         3627 t
ORIGIN      
        1 gtgggggtat gcccccacca aaacacccca aacactcttt ttacgggatt gcgcatgccc
       61 ctcagggcgt atgcaattgc cctgatcggc gctataagcc cgcagtactg aaattccacc
      121 tggtagattg ccaacgattt atagaaaatg acgaaagaat ggttgacaag aatacaccgc
      181 aggcctgatt gaaccaccga ttgaaaggcg atctctcagg ggaaaagggg gaaaaggacg
      241 ggtaaggcaa tcatggtggc gatgaacaag aaaatagtga gcaggaagcc aacgcgggtg
      301 tagtcaaaga atttgtatcc gccgggacca aagacaagaa cgttggcttt atgcccaacc
      361 ggggtgagaa agctggtagc tgcgccgatg acgactgcca atgtaaaggt gagatggttg
      421 gcacccagct ccagggcggt gtctagagcg atagggacga cgagaaccat ggcagctgca
      481 ttggacatgg gctgggtgac gagggcggcg aggatataca tccctgctag agcagcgagg
      541 ggacccaagc caccggtcat gccaaggaag agatctgcaa tataacgggc tgtgccggag
      601 gtctccatcg ccatgccaag aggtagcatg ccagcaacta ggaagacggt acgccagtcg
      661 atgttttgat acgcctcgtc catggtgagg cagcgggtga gcaccatgcc aactgcgccg
      721 atgaccatgg cgatggcaat atccagtttg gcaaagagaa ccaggccaat cacaagaagc
      781 atcaagccga tggcgatggg catgcgcttg cggtggtctt gcgggatttt gataggctca
      841 agggtgagga agtcattccc ctcctgtaaa actggcaaac gatcgcgggt accctggagt
      901 aaaaagtcgt caccaaattt caagctgatg tcgcgtaagc gcttggtgat cagttctcca
      961 tggcggcgga tagccaggac ggtgaatcca tagcgttcac gaaattggag ctcgcgcagg
     1021 ttctgtccaa ccatggttga ataatgagcc aagatggcct cgaaaatctc aaggttggag
     1081 ttgtcgatgt tgtccaaatc catatgggga tggcttgtca agtctagacc gatatccgtt
     1141 gtggcgcgca tcaggctttc taccgagcct tcgatcaaaa gaatgtcatc cggctggata
     1201 accgtgttac ggtcgacggc agccaaggtt ttttcctcac gcacaacagc aatcacagtg
     1261 agatcatagt cagcaccgag tctggattcc agaagtgtgt gaccaaccag tttgcctttt
     1321 gggagaacac gcacttcgct cacataggct ttcaactgtt gcgggatctg ctgctcgcta
     1381 ggggattggc ggatgggcaa aagatgacga ccgatgaaaa ccatatacag gatgccagta
     1441 acgaagacga caatcccgag cgaggtgaag tcaaaaaagc tgaaggtcgg caggccattt
     1501 tcggcgacga tgcttgtcgc taagatgtta gcgggtgtac cgatcaaggt taatttgcca
     1561 cctaagagtg aggagaacga gagcggtatg aggagcttgg agaccgagat cttgttctgg
     1621 cgagagatgc tgacgacagc cggcagcaac atggcggttg cgccgacgtt attcataaag
     1681 gcggagagga tgccgcaggt gagcataatg acggcaatca ggcgcatttc gtttttaccc
     1741 gccagacgca agatgaactt ccccagagta tcagcaactc cggtgttgaa gagaccgccc
     1801 gagacaatat agaccgccca gaccgtgata accgcggaat tggaaaaacc agcaaaaacc
     1861 tctttggggc tgaccagccc tgtaagaccg atagtgagga gcaccagcaa ggcgaccagg
     1921 tcgacgcgca ctttgtcggt gccgaagagc accagagctc caagcacaat tgccagagtg
     1981 agagcaattt caggggtcat ctgttagtcc tataagtcat gagaaaggcc atagaatggg
     2041 aatcaccaat atagcaacga tcatcgtaac gatagtaagt aaaaatccaa ctttgatgta
     2101 atctgcgaag cgataaccgc ctggtcccat gaccagcagg ttagccgcgt gacctaccgg
     2161 gctgagaaaa gcggcggatg cggatacggc aaccgtcatc atcagggggt aaggcgatac
     2221 gcccaggtcg cttgcagtac gcagggcaat gggtgccaat aagaccgcaa ctgctggatt
     2281 gggcattacc tgagaggcta aggctgccac cacaaagaaa ccagccatca ataccatagg
     2341 acccatatta ccgatcaatg ttaccatacc attagccaat atctctgctg cgccagtctg
     2401 ttccatggca atgcccatgg gaagtaagcc tgcaatgaga aagaccgctt tccactcgat
     2461 gtagcgatag gcctcttcca ttgtgaggca attggtcagt atcataaggg caacgccgag
     2521 gatggctgag attgcaatcg gcagccagcc aaagacgaca ggaatcagga caagcgccat
     2581 aataaacagg gcggcaggga ttttttctcg gcggggcggc tcctgggctt cttcggtgag
     2641 gaccaagaaa tcaggttcgc tgcccaacat gcgcaagcgc tcacgcggtc catataacaa
     2701 gagcgcatcg ccaaagcgta gctcggtatc ccgcaaatta gtgcgatagg cacgcccctc
     2761 tctccatatg gcaaggacgg tgagcccgta tttaaagcgg aagttcaatt cgcgcaaggt
     2821 tttcccagct agggttgagt gcggagagag gacggcctcg accagcccta tttcctccga
     2881 ctctagttct cccaggtcgg gcggagggcc attttcaacc tccaagcttt gcaagccttc
     2941 gaccagatgt aaatcgctgt atctgccttt cactatcaag acatcattct cctgcagcct
     3001 ttcatcggga gatggcatca ggttggtctc tccgctacgc atgatgccca gcacacccag
     3061 tccataagaa tcgccgaggt ggctttcgac cagcgatttg ccaaccaagg atgaattagg
     3121 gggcacttga acaaccatca tgcgttcttc gagcttgaaa tcctggagtg agctcggagt
     3181 agagatcgca aaatcagtgt cgtgctttaa ttcttcgata tgttggcggc gaccctgaag
     3241 cagcagtatg tcgtcaccaa gcaatggaat attttccaaa ttggttcgga aaggcacgcc
     3301 ctcgcgcaaa attgccagaa cgatgacggc atagctgtgt cggaatccga tatccctcag
     3361 ggtttggccg atcaagctgg aactggggga gagtcgggct tcaactaact gcacatctgc
     3421 cgaaaccagg cgctcgacag aaaagccttc ttgatccaag ataatgtgat tctttccccg
     3481 caactcggaa aattgctcca aacgcccttc aaccaacagg cggtcgccgg acttcaaggc
     3541 aaaggaaggt tggggagagc gcatgatttg cccttggcgg atgattgcca ccacgttgag
     3601 tccaagcgcc gagcctaggc ggctctcagc gagggtcttt ccattcaaaa tagagccctc
     3661 tggcatatga aggataacca tgtgctcgtg caaatcgtat agcgatttga aatccctggc
     3721 tcgctcagcg gagaagtcgc gggcaaggtc taggtttggc aataggtggc gccctaccag
     3781 gatcatatat accgtgcctg ccagcagggc tgataaacca actggggtgt aatcaaacat
     3841 ctggaacgga cgcaagccag cttcccgcaa ggcttcactg atcaggatat ttggtggtgt
     3901 gccgattagg gtattcaagc cgcctagcag cgaggcaaaa gctagaggca tgaggatttt
     3961 agaaggtggc cgtttcgtgc ggcgggcgat atcgattacc accggcaaga acaaggagac
     4021 gacaccaacc gtgtttataa aaccagagag gatgccagca gttatgctaa ggaagagcaa
     4081 taagcggact tcgctatctc ccgctaagcg cagcatatag cgtccaatca agcttgccac
     4141 cccagtgcga gccaatccgc cactaaggat caggatcgcc caaactgtaa ccactgcagg
     4201 gttgctaaaa ccggagagcg cctgcgctgg ggtgacgaga cccgccaccg ccagacctcc
     4261 aagcaccaga aaggcaacca cgtccatgcg cacccgttcg gtgacaaaga gaaggatggc
     4321 aattgctaag atggctaaga caaggatgat ttcaaccgtc atggcatatc caactggtcg
     4381 ctatgcactt acttgccgtt acgagcaagc tatgtattac gatttttgcc tgaaactgta
     4441 tcccattaag cataataagc gcatgtgctg caatcaagcg gttttgaaat agttccgggt
     4501 tggttagaaa ttatacatgg taaaggatat atgcgtggtg gatggccaca aagagtaggg
     4561 caattgcata tgccctgagg ggcatgcgca atcccccaaa cactctcttc agtggtggga
     4621 aatcgtccta atgcgattgc cctaccacaa agggttgtgg caagtatttg tgaggtaaga
     4681 tgtggtacag catcggcttc acattattgt ttgtttatcc caatgcaact ggcttataat
     4741 ttgagtgtgc cgacgccatt ctaccatctc agtgtagccg aggacttgct cgggcagccg
     4801 cgcttgggca aacaggcgcg cctcctgctg gaggctcagc gcagcgcgtt cctgttcgga
     4861 aataccgcgc cagatgtgca ggtgatctct caccaaccga ggcaggatac gcattttttt
     4921 gacctgccgc ttcgctcgct agcaccatcg ccctgggagc tgttgctccg tgattaccct
     4981 tcgctggcca gggcggatct attgcctcct gcgcaggcag catttttagc tgggtatctt
     5041 tgccatttac aagctgactg gctgtgggtg aaggatattt tcgtgcccgt ctttggattg
     5101 cacagtacct ggggcagttt cccccagcga ctttatctgc ataacgtctt gcgggcttac
     5161 ctggataggc atatcctgcc cagccttgca aatggggcat gtaccagcct ggctgaagcc
     5221 agacctgctg gatggctgcc ttttgtccat gagcggtact tatacgaatg gcgcgacttc
     5281 ctagtaaccc agctccagcc acaagcttcg gtccagactg tcgaggtgtt tgccgcccgt
     5341 caaggcattt ctcctagcga gttttaccac ctgctcgact cagaagaggc aatggaacgg
     5401 gaagtcttcg cccgcatccc gcgacaagcc ctagagaatt atcgcgagat tttgatcgct
     5461 gagaatgcca agctcattca taattacatt gcccgtgcca cggcgatacc ataagatgca
     5521 ttatcgatag gagacttatc aaatgaaagt tattgataat cctgagttta gagagaagcc
     5581 cggtacccct gccaagctcg attctgtgct tgactcgctg gggatgggaa ggagtgggtc
     5641 acctgagctg cagtcgcagg aggatttgat cgcctctttg gaaaagaccc tggataaccg
     5701 ctttgtgtta ttacgcaatg tcactctaga agacctggat acccccatcc cgctggtgct
     5761 gttggggccg tcgggcgtga gcgtcatctt ccctagccca gcgcgaggcg tgttcagggc
     5821 taaggaagaa accctggaat acatggatga atataaccgg gtctatcggc aggcaaatcc
     5881 gaacctgctg gcgcgcacca tcatgatggc taagtcagcg gaggcctatt taaacagcaa
     5941 cttccctgcc ccgattgagg tcgagccggt gttgtatttc tctgatccgg ggacgcacgt
     6001 cgaatcggtg agaccgtccc tgcgcatcct gttggtggac gggctggagc gtttcatcgc
     6061 tggacagttg caggctgctg ttttatatga gagggaagag gttcagaaac tggtggatct
     6121 tctcctgaaa gccagcagga ttaccgagcg ccaagtatcg ccttatccag aacgggatgc
     6181 cttttccttc gtcgatgaaa gcggattgga taagccctcg atcgccgatc ggctgccgcg
     6241 tggcgagggg atcgtctcag ccctcaacaa aatccccttt tccggtcgcc agtggctttt
     6301 gctaggatgt atggtcgtgg tcaatgtcgt cgtcttgatt gcgttcgtgt tgctggtgtt
     6361 gttcacttcc tgattagagg tctatatcat tgaccggacc ttttctctcc attgtcatcc
     6421 cggcttataa cgaagaaaaa cgcctgccaa aaactctgga gcaagtgcgc tcgttcttag
     6481 aaagccagcc atataccgct gaagtactgg tcgtggaaaa tggcagtcag gaccgaacat
     6541 ttgaggtggc gcaagacttc gcgcggcggc acccaggagt aagtgtcctg caggaaaccg
     6601 ggcggggcaa agggcgcgcc gtgcgacgag gtatgctggc ggcgcgcggt gaatatcgtt
     6661 ttatgtgcga tgctgacctg tcgatgccgg tggaagagat caattatttc ttaccacctg
     6721 ccttgcaaga ttttgatatc gccattggct cgcgcgaggc gcctggtgcg attcgctata
     6781 acgaaccgca ttaccgtcac ctgggaggcc gggcggtcaa taccatgatc cgcttgctgg
     6841 cgctgcccgg actgcaagat acgcagtgcg gcttcaaatg ttttcgcgcc ccgattgcag
     6901 aggagctcta tcgactacag accctcactg gttggtcgtt tgacatcgag ctcttgtata
     6961 tcgcccgcca gcgcggttat cgcatagtag aaattcctgt tcaatggtat tttaatcctg
     7021 agagtaagct caacgttgtg caggatgcca taaaaatggg gctggacatt ttcaccatcc
     7081 atctgaataa tatgaagggc gtttatgacg ccaaggtttg acgtatctct gctgccgcct
     7141 gcgccgaact tcgatttcga aatcgccctg tgggaaaggg gctgctgcta cgtggcgggc
     7201 gtggatgaag ccgggcgcgg agcgctggct ggcccggttg cggcaggcgt tgttatgttt
     7261 cctgacgatc ctggtctgct acccgagttg gtcggcgtgc gcgactccaa gcagatgacc
     7321 cattcggagc gcgccatgtg ggctgagcga cttaagcagc tagccctgac gtgggctgtg
     7381 ggcttcgcct cggtggagga gatcgatagc ctgggaattg tgcctgccac tcggctggcg
     7441 gctcgccgcg ccacgcgcgc cctgggtgaa cagcctgagc acctgctggt ggatttcctg
     7501 gagctgcccg agctgccgat cccgcagacc ccactggtca aaggggatag gcgctcgttg
     7561 agcatcgctg cagcttctat cctggccaag acggagcggg atgggttgct gtgtgagctg
     7621 gaggcacgct accccggcta tggcttggcg cagcacaagg gctacgccac ccaggcgcac
     7681 cgagatgcat tgaggcgcct gggccccgcg ccgatccacc gacacagttt cagccctgtt
     7741 tcatcccttt ccccgcttgc tccccttccc ggcggaagcg gagaaaagac aaaaacgtga
     7801 tttcccggct gcgaattagc cggaaacatc acatacggag gcattatggt acgagcaaaa
     7861 acccggcgcg cctatggttt ttgggacagc ccgcttacgc cgaagcgcat ggcaggcggc
     7921 ctgcacctca atgatgcccg ctgggatgag agcggtgcgc tggtgtggct ggaagggcgc
     7981 tccgagcggg cagccctggt cgtccaagcg cctggaggcg gtgcgccccg cgatttaaac
     8041 agtgaatact cagtgcgggc gaaggtgggc tacggtggtg gcgattttgg cgtgggtcat
     8101 ggccaggtct attttgtaga gtctacctcc gggcgcatct accgccagtc tctggaatac
     8161 gggctggccc aaccagtaac tccggctttc gggcacgcgg ctgccccaac cccctcaccg
     8221 gatggcgatt ggctgctctt cgtccacacc tatgagggcc aggatagcct gggcatcgct
     8281 ggcacgcagg gcaggttctg gccgggcagg ctggtctctg gcgacgattt ctacatgcag
     8341 cccacctggc atccagacgg caaacaggtg gcctggattg cctggaacca tccgaacatg
     8401 ccctgggatg gcacgacctt gtggctgggc aacctgacct ttccaccaaa cggcttgcct
     8461 ttgatgggcg aggcgaaacc cctggctggg gacgagaaca ctgccatctt ccagccggaa
     8521 ttctcgcccg atgggcgcac cctggcttac gtctctgatg ccagcggttg gtggcaaata
     8581 tatctttacg acctggagag tggtgcgact cgccaactga cggatgagca ggcggagcac
     8641 ggcgcgccag cttgggtgca ggggatgcgc acctatgctt ttggcccggg aggggtgagc
     8701 ctgtacttta tccgcaacca ggctggattt gccagcttgt ggaaggcaga tgtggcctcc
     8761 gggcggcttt caaagctgga gctacccgca cagtacacct ggctggagaa gatcgcagtc
     8821 tccccagtgg atgggcagat tgctttgatt gcctcaggtg gagatacacc cccgcggctg
     8881 attgtccagc atgctgatgg aggcacgcac ctgctccgcc actcgcaggc tgaggacgtg
     8941 cccgtatcgg cttacactct gccgcaggcg atcacctggc agggtatgga tgacggtacg
     9001 atacatggtc tttattaccc gccacacagc gagacgtttg aaggaatcgg cttgccccca
     9061 ttgatggtgc tgatccatgg tggcccaacc agccagcgca tggctagttt cgatgaggag
     9121 gtgcagttct tcaccagccg aggctacgcc gtgcttcagg tgaactaccg gggcagcact
     9181 ggttatggtc gtttgtatcg aaacatgctg cgcggcaatt ggggcattta cgatgtgcag
     9241 gatgcggtca gcggggcgcg ctatcttgtg gagcagggca gggtagaggg tagccgaatg
     9301 gtcatcatgg ggggcagtgc gggtggctat accgtgctgc tggcgttaga gcaattcccg
     9361 ggtttcttca aagcagccgt aaatctctac ggcgttacaa accagttcga cttcgttgcc
     9421 ggaggcacac acaaattcga ggagcgctac aacgatacct tgctgggtcc ctatcccgag
     9481 gcggggcagg tttatcgcga gcgctcgccg atcttcttcg tggacaagat tcaagacccc
     9541 gtggcggtct tccaggggga ggacgacaac gtggtaccgc gccgccactc ggatgaatta
     9601 gtcaagtcat tgcagcggcg tggcgtgccg cacatatacc atgtctaccc aggcgaaggg
     9661 cacgggttct cgaaacctga gacgatcgag catttatatc aggagttgga taagtttctc
     9721 aagcaatatg tgctcctgac atgaaggatg ggggttcagg tggagcatat gtttatggga
     9781 ggagcacttc ttcttaaata tgggttgatt gcgtgcaagc cgcgccgccc caaataattt
     9841 gggaatagag aatagaagaa gatactgggt gggagtttag caatgcttca ccgaactagc
     9901 tggcgcctct tctgctcctg ctgccaggct cctaccagcg agtctagctc agtgctgaga
     9961 gccatccact cgggcaccag cagcacgaag cccttagcgc gccggtagaa catttgcggg
    10021 gcgaggaata agcgcagcaa gacggggggc agcgggtaat tattcagctc cactacaacg
    10081 gcggtcatgc cgtagaatgg ctcgagcagg cggcgctggg cccagctggc gttcttaatt
    10141 gggaagatct gggtgaaatc cgctggatgg gtgctgacca ggcggcggta ggagatgttg
    10201 gtgcgcagaa agggggtgac caaccgcaaa tggtcgcgtc tcggctggac ataagccata
    10261 ccgcgggcga agaaggcaaa caaggtaaag ccaagcaaga caaccgctcc caacagcaac
    10321 cacaagtcgt cttgcccgtg gagcaggctg gtgccatacc accacgtcca aaaccagatg
    10381 ccgaagagca gcagacccag gatcagggtc gatctccaca ggcggtccat ccagcgcctg
    10441 tagagcagca agaggtggcg cgagccagct tgacgagctt tcataggtat ctttctccca
    10501 tcaccctgcc cccttcccct agaggcgtgg caatcatccc tcggtcattt tgcgtagacc
    10561 atgaagtgcg agcggctctt cttccccttg ggtttaataa attaagcggc tgatgaaagc
    10621 tgcgcatagt aatcacatag atcctgaatg gggcaggtgg ggcaattggg gcggcgcgcc
    10681 tggcaggtct cacggcccag ccggatcaga ttaagatggg cagcgtagta ggtctctggc
    10741 gggagcagct cagccaggtg ctcgtgagct tgttcggcat tcatcttggc tgcgcgcagg
    10801 ccgatgcgcc cgctcacccg gtagatgtgc gtatccaccg ggaaggctgg cttgcccaag
    10861 gagaagagca gcacgatggc tgctgttttc ggtcccacgc ccttgaagct catcaaccag
    10921 gcgctggctt cggcagggga taggtcattt aaaaaatcca ggctgaggct gcctcgctga
    10981 agggtgatct ctcgcagcac ctgctggatg cgcggcccct tctggttggc tagacccgcc
    11041 gggcggatgg cctgaataat ttcatctgtt ggggcgtcgc gcacctgctc ccaggagggg
    11101 aactggcggc gcagcgcctc aaaggcgcgg tctcggttga gatcattggt gttttgggag
    11161 aggatggtgg agaccagctc gtctagcggc ggcaggtggt tgcgccattg cggatgacca
    11221 tagcgcttga tgagcctttg atggacagcg agggcgcggc tggcttgatc catgctcacc
    11281 tgcttagctg aaagcccgga cgcaaccgac cgacttcaag cctttgccag ttgtacactc
    11341 cggtgcttgg cccatattct gaaagccgat ccatttccga tgactctagc acgcccagct
    11401 ggtgcaggac ttcaaggaca accgcgggtc tcgctcgctg gcgcgcgtct ccatcggaga
    11461 ctttgatggc tatgcctgct cccggcgagt ttgggctgag cgttccagga cgcaggccaa
    11521 gaccaaagta gccttccgca ccagactttg ccaggatgcg gccgcctgtg gcttgcatca
    11581 ggcgggtatc gaagctgtct ggcccgccga tcatctcggg gtgggatgtc atcgccgcta
    11641 ggatagtatg gcatgcaact tggcgagacg gtgggagtat cccgccggtc tccgggtcgc
    11701 acaagcggcc cagcccaagg gcagcattga agagcggcac agcaaagttt ggggcggagc
    11761 agccgtcggt gccgatctca atctgttcaa ccgtcacttt gcacatggca gcgaatgtgt
    11821 tcaggatatc ttgctggata gggtggtggg ggttgagata atcttctgtg ggcaaagcct
    11881 tcatgcgggc gtaggccagc atgccggtat gtttgccgga gcagttgttg cggttggggg
    11941 tgacctgttc acttcgctgg cgcatcgcct cgagggttgg ctcatggctt agtgggtgaa
    12001 cgccacattg cagatcagcc tcgctgaccc cggtcttctt ctgcaagccc tgtaccacgg
    12061 caacctgctc gttcgtaccc gagtgcgagg cgcacatcac agcgatttct cgctggctca
    12121 gtttatagaa tttgtgcccg tcgttctcaa tgaacggcag cgcttggaag ggtttggcag
    12181 tcgagcgcag gtaggagacc gcctgcgggt cgccgtagct ggcgatcaag tttccccggg
    12241 cgtccaccac ggcaatggca ccgaagtgga tcgattcaac gatcgagccg cgggttagtt
    12301 ccaacaaggg caagtagtgc gctgcatgca taagctgtct attatattaa ctatcgctgt
    12361 cttcgaaatc gagctggtca ggcagcgtct cctggcggta gtggtggatg taatattgga
    12421 acaaaccaga gcgtaagcgt tgcgtgtact ggtcgctgaa cttgtccgga taatctacct
    12481 ctttaagaag tgagttgatc agattttctg ttgcttcagg tagagcattg cgtttagcta
    12541 ggcgctccag ccctttatag atattcttta gatagcgttg ctgggctcgc actgcatctg
    12601 gctcaaccag tcccccacgc ccgctgacga tcgtgtagcc cttgaaagta ttttccaggt
    12661 ggtccagcgt ttcgatccaa ataggaaggt cggcttggga gaggaaggga ggctggttgg
    12721 gtgttaccgc gtcgcctacg aagagcacac cagcctgggg gatgaccacc cagatggagc
    12781 ccgacgccgg gccgggatga tgctccagta tgacctccgg tccaccccag tacaggatca
    12841 tcttttcaga gaaggtgata tcaggagata tccagcgtgt gccaataaca tcatcgtagg
    12901 tctcccaatc agcaccgcct tctgcgctct gccccttgaa gatcaccggg cggttgcgga
    12961 agacctgggc ggtcttatgg tgcgagatta cggtacattc catggcacgc gtgcccagag
    13021 tgcgatctgg gtgggcatct agcgagatca gcaagcggga agatccacct cgtagggtga
    13081 tcaaagccga gcgccaggca cgcgtgtctt cggtgcgtag cggggcgtcg atcatgatcg
    13141 tcccttgaga gaagatcaag gcgcccaatg tcacacccag ataagcatct tcgtagaata
    13201 gtcctggttt aagctggtgc atatatctcc aatattatat cgaagggcca tccggcttga
    13261 gcgggaggaa ttcctaatct gggagtaaat cccgcatatc ccgaactcga tcagagcaaa
    13321 ccctcgccat cgctcaatcc atctgagtgg gtagggtgaa tttaaagttc gaaccacctt
    13381 ctggtccgtc atccacccaa atctgcccac cgtgggcctc cacagctaat cggcagaagg
    13441 ccaaccccaa acctaatccc tttggaccac cccgtccgtg cagacgggaa aatttatcga
    13501 aaatgcgctc gcgttcatcg ggcggaatgc ctggaccgtt atcttgcacc cacattatta
    13561 cttggttgcc ttccaggcga gcgcccaggt agatggtgct cccagcaggt gtgtgcttga
    13621 cagcattctc caataggttt gtcagcacgc gtccgatcat atcggtatct acccagactc
    13681 tcggtagatt ctcttcgatc agcaacgtga tctcctgatt cttgttctcc acgatgggta
    13741 gcacgttttt aactgcttct ttggtgatgc tagtggggga aacgagctcg cggctggcaa
    13801 tggactgacc tgcctccagg cggcggatgt ccagcaacga attggtcagg cgctcgatac
    13861 gctcagtggc gcggatggcg atatccagca agggctcaac tgcgggatcg gaatcttggg
    13921 gcatcaatgc ctggatgaca tccaggctgg agacgatgtt ggacagtggc gagcgtagat
    13981 cgtgataaat catggagatg agatcgttac gcaggttatc cagattttta cgctcggtaa
    14041 cgtcccgtaa aatccattgg aggtttgaga taccatcaat gaagacgttg cgaacataga
    14101 cttggatagg gacttcgcga ccgccttctg cctttaggca agtttcatag ctggtggttt
    14161 cgccagaggc gagtttttcg aatttctcgc cgagctcagc ctgatcgacg atttgaaaat
    14221 ggtcgatcat ggcgttgagt agcgactccc gattcatacc ggtgatcatg gcaacctggc
    14281 ggttagcctc caggatacgc ccttccaaat cagtgatcaa aattgggtcg atgctgtctt
    14341 caaataattc gcggtatcgc tggtgggcag cttccatgcg ttcgaagagc tgggcgtggt
    14401 ggatggcagt gccagccaat ccgccgatac ctgttataac ctgcagggcg tctgggtcga
    14461 aagcgccatc tttcgggttg atggcttgca ggatgccaat cacctcgcct cgcaagcgga
    14521 tgggtgcaca ggcgatggct ttggtgacaa aattgagtag tttgtcgaca gtagggagaa
    14581 agcgcggatc ttggcgcaca tcgggcacga ttattccgat gccttctctt gccacccaac
    14641 cggcaatacc ctcacctgga gccaggcgaa tgccgataac atttgggcct ttctcaccag
    14701 acgcagcgcg gaagaccaac tcctgggttt gcggatcgat caatgccagg gagaccactt
    14761 ctacctgtag agcttggctg atctgctcaa ggatttgctg caggacatca tcggttttta
    14821 aggatgccgt gatggtggca gcgctctctg ccagggcggt catggcgcgc gtctggcgct
    14881 ggctctctgc atacaggcgg gcattgagca cagcgacgcc cgcctggtcg gtaatagctc
    14941 gcaccagagc caggtgatcc tgggtgaaga aacccggggt ggggtgcacc agggtcatca
    15001 cccccaccag atgatcccgc gccagtatgg gcgcgctgac agcagatttt ggaccggtgc
    15061 gttcggcgtc atcatccgga cggcgcagcc agcgctcgtc ttggctggta tctggcacca
    15121 gaacagcttt ctgattgcga atcacccaac cggctaagcc gcgctcgtag gtgatgcgca
    15181 gttgttgggt ggtttgttcg tgtaattgcg agccggtgat gatggcagac tcaactggtt
    15241 gctggttgcc gtccagcacg atgatgctgc cgctgattgc cctcacgttc ttcatggaca
    15301 ggaagagcac gcggtggagt acggtgcgca ggtctaaggc agctgcaatc tctcggctga
    15361 catcgtagag cagctcgaga gaggctagca tactctggtc ttgtgctttt ttatccatca
    15421 atcagggtct cgtatttgcg tttgagtata gcacatttgc aatacttgag aaagttggtt
    15481 cccgggtgat gaatgtcatc cccaaaatat gcaagtaatc acttgcttgc actaagcagc
    15541 agggtataat atgatgcaag tgctcacttg catgtcaata caggagaatg aattatgtca
    15601 cccaatacca acccggagag agaacctggc gacacacgac agcgtatcat ccaggcagcc
    15661 attcagctgt ttgggaaaca gggctactcc caaaccagca cacgcgctat tgccgaagcc
    15721 gctggggtca acgaggtcac cctcttccgc caattcggac ggaagaagga tctgttgatc
    15781 gcctgcatcg aagcccataa cgctgccggt ttttccgcaa cgtttgaatc ggaactgact
    15841 ggggattatg cagaggatat cttgcgcatg gcggagttac aaattcggga tacgacggca
    15901 aacctggaaa tcctgcgcct gctgtcttgt gacgcgcaca atatgccgga gctacgccag
    15961 gcgatgctgg caggtgggcg cggcaacctg gcccgcctga gtcgctattt ccagcggcag
    16021 atcgatcgcg gggtagtccg ggcagacctg ccagcggaag tgctggcatc tgcgttcgat
    16081 agcctgtttt cctccaacat agtttttgaa tacttgtttc aaggcaatct ttcacccagg
    16141 ctgacaacga atgaagtaat tcgcccgctt gcagcgctgt ttgtgcgcgg cacccaggcg
    16201 attgatcaaa ggagcgtttg agatgttacg gattcttacg atcgtgttgg cagtcttgac
    16261 cggaatccac ggcctgatcc acctgatggg cttcgcagct tactggccat tggcgaaaat
    16321 caacgagctt ccctataaaa ccagcttgct ggcaggacgg ctggaggtgg gcatggcggg
    16381 aatgcgggtt ttcagcttct tgtggttgct ggcgacgctt ggctttgtga tcgcagctat
    16441 tgcactagcc tttggtaaat cgaattgggc g
//
LOCUS       DBNHOH010000068         4038 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1285005, whole genome shotgun sequence.
ACCESSION   DBNHOH010000068 DBNHOH010000000
VERSION     DBNHOH010000068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1285005"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="AC16V7_01205"
     CDS             complement(<1..292)
                     /locus_tag="AC16V7_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255762.1"
                     /translation="MKKISYLPIVMGIIACLCLAVGAFIWATGLIDSVYAYRSPLHDN
                     PPTPGKPVGPPLTSSVVFVLVDALRLDTSLKPDIMPFLNELRSQGAWAVMHSR"
     gene            complement(279..1184)
                     /locus_tag="AC16V7_01210"
     CDS             complement(279..1184)
                     /locus_tag="AC16V7_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014515790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate 5-dehydrogenase"
                     /protein_id="HIR6255763.1"
                     /translation="MKRAVSISLGSSDRDKRVVVQLKNELIEVERIGTDGDVGKAIRL
                     FTELDGKVDVLGVGGVDLYLRLDQREYPLYSALQLVKNVRQTPLVDGRGLKHTLERRV
                     FELAEPALGGMPYFRYAFVPVAVDRLGLAYAAESVSDRVVIGDIMIALGIPIPLMGIR
                     AYKKVARILLPAVSYFPMSMLFYGSGGAEHQPKYMQYWNKADLLAGDFLFMRKYMPLD
                     LTGKTIVTNTTTPENVDFLRRRGVQAVITTTPRYEGRSFGTNMMEAALTAYAGKGRSL
                     TDEELNQLIDELDLRPNVMVFDEKD"
     gene            1319..2146
                     /locus_tag="AC16V7_01215"
     CDS             1319..2146
                     /locus_tag="AC16V7_01215"
                     /inference="COORDINATES: protein motif:HMM:NF025615.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HIR6255764.1"
                     /translation="MKNGITQNYLRRHIAIPSEHGSWVFVLSPLLIGLFAGGRWVTGS
                     FYLILAAMAAFLIRQPVTIAVKAYSGRRSKRDLPAAWFWIIAYGLIGLVAFALLTAQG
                     FGSLLLLALPGIPVFIWHLYLVSKRAERGQAGVEMVASGVLALAAPAAFWVGVGEPDP
                     SGWWLFALVWMQSAASIVYAYLRLAQRELASVPESIIRLRMGRRALLYTTFNLAWVIF
                     FSIFRVFPPLLPLPYLLQWVETLFGTLRPAVGVKPTSIGIRQLLISTLFTVLFIMTW"
     gene            2287..2628
                     /locus_tag="AC16V7_01220"
     CDS             2287..2628
                     /locus_tag="AC16V7_01220"
                     /inference="COORDINATES: protein motif:HMM:NF021221.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HIR6255765.1"
                     /translation="MTDQPAEQTTNDITSDDKLWSLLGYILSPIVPIIVLLLEDKKSR
                     PFIRYHNMQALVLGVVNIILSLILGWTLVLACVPFLIWVVMVYWGIQAYQGQWVKIPV
                     LTDFVKNQGWA"
     gene            2716..3153
                     /locus_tag="AC16V7_01225"
     CDS             2716..3153
                     /locus_tag="AC16V7_01225"
                     /inference="COORDINATES: protein motif:HMM:NF021221.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HIR6255766.1"
                     /translation="MDVNTGTGSPESLSPSDERTWAMLAHLTVLANLVTGFLGTVAAL
                     VIYLVYKERSRYVAYHSMQAFIFQLVWWIGGGALTGIAWTFTGISSIALIGLLCIPLA
                     CLVSLVPLGSIVYGIIGAIQCSQGQDFRYWLVGDWVRGTLTGE"
     regulatory      3233..3337
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3496..3885
                     /gene="gcvH"
                     /locus_tag="AC16V7_01230"
     CDS             3496..3885
                     /gene="gcvH"
                     /locus_tag="AC16V7_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HIR6255767.1"
                     /translation="MNIPSDLKYTSNDEWIRIEGNIGTIGITDYAQDQLSDIVFVEVI
                     VSVGESVKKGDSCATIESVKAAADVYMPASGKILEVNDSLPDTPEVVNNDPYGAAWMV
                     KFEINDPSELDQLLDSTAYEKNIAEKE"
BASE COUNT          980 a         1087 c          964 g         1007 t
ORIGIN      
        1 gccggctgtg catgactgcc caggcaccct ggctgcgcag ctcattcaag aatggcatga
       61 tatccggctt taaagacgta tccaaacgca aggcatcgac gagcacaaat accacgctgc
      121 tggtcaacgg cggtccaacc ggcttcccgg gtgtcggcgg gttgtcatgc aagggcgatc
      181 gataggcata taccgagtca attaggcctg ttgcccaaat aaacgcaccg acagccaagc
      241 aaaggcacgc gattatgccc atgacaatcg gcagatagct aatctttttc atcaaatacc
      301 atcacattcg ggcgaagatc cagttcatcg atcaattggt ttaactcctc atcggtcagt
      361 gagcgacctt tgcctgcata ggccgtcaag gctgcctcca tcatatttgt gccaaaggaa
      421 cgcccctcat aacgaggcgt ggttgtgatc acagcttgca caccacgccg gcgcaagaaa
      481 tcaacatttt ccggtgtcgt tgtgtttgtg acaattgttt tgcctgtcaa atcaagcggc
      541 atgtacttcc tcatgaataa gaaatcccca gccagaagat cggccttatt ccaatactgc
      601 atatatttag gctgatgctc cgcgcctccg ctaccataaa agagcatgct catcggaaaa
      661 taactaacag caggtaacaa aatccgcgcc acctttttat atgctcgaat gcccatcaaa
      721 gggataggga tgcccaatgc gatcattata tcgccgataa ctactcggtc tgagacactt
      781 tcagccgcgt atgccaaccc gagacgatcc acagcaaccg gcacgaaagc atagcgaaaa
      841 taaggcattc cgccgagagc cggttcagca agctcgaaaa cccgccgctc aagggtgtgc
      901 ttcaagcccc gcccatccac caaaggtgtt tgacgaacgt tcttaaccag ctgtagtgcg
      961 gagtacaacg ggtattcacg ctggtccaat cgcaggtaca ggtcaacccc gcccacaccc
     1021 aacacgtcaa cttttccgtc tagttccgta aacaaacgga tagcttttcc tacatcacca
     1081 tccgtgccga ttcgctctac ttcgatgagt tcatttttta actgaacaac aacgcgttta
     1141 tctcggtctg agctccccaa gctgatgctg acggcacgtt tcataacgct ccaaaaggac
     1201 agatctatat cttgccattc caagaatgtt gctagttgac tggaagatta tattacaagt
     1261 cagaccaatc tcacccctcg taagggacta tcatgccaaa tcagctataa ttctccccgt
     1321 gaaaaatggc atcacacaaa attacttgcg ccggcatatt gccattccaa gcgaacatgg
     1381 atcctgggta ttcgttctaa gtccgctatt aattggtctg ttcgccggag ggcgctgggt
     1441 aactgggtca ttctatctca tcttagctgc tatggcagcc ttcctcatcc gccagccagt
     1501 gaccatcgcg gtaaaagcct acagcggtcg ccgctcgaaa cgcgatctac cagcggcatg
     1561 gttctggatc atcgcttatg ggctaattgg gttggtagca tttgccttgc ttactgccca
     1621 aggatttggc tccctgttgc tcttagcctt gcctggcata ccagtattta tctggcatct
     1681 ctatttggtt agcaagcgtg ctgagcgcgg ccaagctggc gtggaaatgg tcgccagcgg
     1741 tgtgctggca ttggctgcac cagccgcctt ctgggtgggc gtgggcgagc ctgacccatc
     1801 cggctggtgg cttttcgccc tggtttggat gcaatcggca gcctctatcg tatatgccta
     1861 cctgcgcctg gctcagcggg agctggcctc tgtccccgaa tctatcatcc gtctgcgcat
     1921 gggacgcagg gcattgctat acaccacctt caatctggca tgggtaattt tcttctccat
     1981 cttcagggta ttccccccac tgctccccct tccttacctg cttcaatggg tcgagacatt
     2041 gttcggtacg ctccgaccgg cagtcggtgt taaacccact agtattggga tacgccagtt
     2101 gctcatcagc accttgttca ccgtgctctt catcatgacc tggtaatccc accttgcaaa
     2161 cctgatagca accctaacca acttttgggg ttattagata gagagatcac ttgctttgca
     2221 ttcaacctct tgtactatgc ttgcacagtg ctccaaaaca ccatcttttc cataggaggc
     2281 aatcaaatga ccgaccaacc cgccgaacag accaccaacg acataaccag cgatgacaag
     2341 ctttggtcct tgctgggcta cattctttcc cctatcgttc ccatcatcgt gctcctcctt
     2401 gaggataaga agagtcggcc ttttatccgc taccacaaca tgcaagcgct tgttcttgga
     2461 gttgtcaata ttatcctctc tctaattctc ggatggacgc tggtccttgc atgtgtaccc
     2521 ttcctgattt gggtcgtcat ggtctattgg ggcattcagg cataccaggg gcaatgggtt
     2581 aaaattcctg tcctgaccga tttcgtaaag aaccagggtt gggcataact atttgataca
     2641 tttttcagcc ccgtcttcat acccattccg aagacggggc tatttgtgtc gcaaatctca
     2701 aaacaaggag aaaaaatgga cgtaaatacc gggacaggtt cgcctgagtc actctcccca
     2761 tcagacgaac gcacctgggc tatgctggct cacttgaccg tgctggccaa cctggtcact
     2821 ggatttttag gcacggttgc ggcgctggtt atttacctgg tttataaaga acgctcgcgt
     2881 tatgtcgcat accattccat gcaggctttc attttccaat tagtctggtg gataggaggc
     2941 ggcgctctaa cgggtattgc ctggactttc actggcatct catcaatcgc cctgatcggg
     3001 ttattgtgca ttccattggc ttgcctggtc agcttagtgc cactgggctc gattgtgtat
     3061 ggcatcattg gcgccatcca gtgcagccag ggacaggatt tccgctattg gctggtgggc
     3121 gattgggtgc gtggcacact aacgggtgag taacccgacc cccttacata ggcggggacg
     3181 aattacactt gacttttagc gtactcaccc ttagaatgat ggcgaacaat ttaacgggat
     3241 cagggaagag actcgggtaa aacgagcacc gaaggggcaa atctggcaga aagcaatgcc
     3301 aggtgaaact ctcaggtaaa aggaccctga tgctggaacc ctctggaaag tctggcaggt
     3361 tatcacaacc cgcagtacgc tagcctgcac accgacgggg caagcgcgac acccagcgcg
     3421 aatctctcag gttcaagaca gaggcgcacg caatctacgc gtgcgccttt tattattttt
     3481 ctccaggaga ctcacatgaa catccccagc gatttgaaat acacctccaa tgacgaatgg
     3541 atcagaattg aaggcaatat tggcaccatt ggcataaccg attatgccca ggatcagctc
     3601 tcggacattg tgtttgtcga ggttatcgta agcgtgggtg aaagtgtaaa gaaaggtgat
     3661 tcttgcgcta ccatcgagtc ggtgaaagct gctgccgatg tgtatatgcc agccagcgga
     3721 aagatcctcg aggtgaatga ctctcttcca gataccccag aagttgtcaa caacgaccca
     3781 tatggcgctg cttggatggt gaagttcgag attaatgatc ctagtgagct ggatcagttg
     3841 ctcgattcaa ccgcatacga aaagaatatc gcggaaaaag aataataagt gcgtggtgcg
     3901 ggctacagat taatcctcca ccctcgccat ggattgacaa aggagttcgt atgttctttc
     3961 cccatactga tgaagatcgc aagtccatgc tggaaacgat aggtgtgcag cgaattgaga
     4021 atcttttcaa tgcagttc
//
LOCUS       DBNHOH010000069         1728 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_195582, whole genome shotgun sequence.
ACCESSION   DBNHOH010000069 DBNHOH010000000
VERSION     DBNHOH010000069.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_195582"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            444..>1728
                     /locus_tag="AC16V7_01235"
     CDS             444..>1728
                     /locus_tag="AC16V7_01235"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020527435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR6255768.1"
                     /translation="MTIESALETSEEAPDLGLSPQEILSDYRLAYRSRQVSLIARREV
                     MSGKAKFGIFGDGKEIVQLAMAKVFRKGDWRSGYYRDQTWMFAVGITSIQEFFAQLYA
                     HTDVDADPATAGRAMNAHFGTRSLNPDGSWKDLTQMLNSSVDISPTAAQMPRLVGLAY
                     ASRLFRELPELQGFNQLSRGGSEVAFGTIGNASCAEGLFWESLNAIGVLGAPALITIY
                     DDGYGISVPNEQQIIKTNISELLQGFQRDPERCGGYDNCGYDIYTVPGWDYPRLVQTY
                     RKAEASVRDEHVPAIVHVNELTQPQGHSTSGSHERYKTNERLEWEREFDGLKKFREWI
                     IAQDIASADVLDEMDAEDRGQVEEIRKQAWQTYIAPIEGERAELVRLIEQMAQGSSQA
                     SELSAIQRKLINTPNPLRRDLMVATREALMAVRGEQ"
BASE COUNT          422 a          406 c          520 g          380 t
ORIGIN      
        1 agattggaag gagtggtcat aagtggtata taccagattg tagacaaaaa aattatctat
       61 gtcaatagtt tttttctgga tggcggatgg atttgggacg cgggaggtgt cgagtagcat
      121 gaaccacatg cgaagtacat catgcgacct gcgataagcg aatttcacac aagggggaga
      181 ttgttacctc gagatattcg gggcggctat tcgtaacacc aagagtacaa attaaaaact
      241 ctttaccctt tgcacctcct tcgtgtcctt tgtgtttaat tattactttg tgcaactggc
      301 ggcttctttc tcaggacaag gctgcagcaa atttataaaa cactcaccga acaagcccaa
      361 aaagggagat ataatcttaa acacctaatc gatggctgtg cgggtttggt ttagatgggg
      421 aaaggttttt aggaggtatt gcgatgacga tcgagtcagc gcttgagaca agtgaggagg
      481 cgcctgacct cgggcttagc ccacaggaga tattaagtga ttaccggctg gcatatcgca
      541 gccggcaggt cagcctgatc gcacggcggg aggtgatgag cggcaaggcc aagtttggca
      601 ttttcgggga tggtaaagag atcgtgcaac tggcgatggc caaggttttt cgtaaggggg
      661 attggcgctc gggatactac cgcgaccaga cctggatgtt tgccgtgggt atcaccagca
      721 tccaggaatt ttttgcccag ctatatgccc atacggatgt ggacgccgac cccgccacag
      781 cgggacgggc gatgaatgcc cactttggaa cgcgcagctt gaatccagat gggagctgga
      841 aagacctcac gcaaatgctg aactcgtcgg tagatatctc gcccactgct gcgcagatgc
      901 ccaggctggt cggtctggcg tatgcctcta ggctgtttcg tgagctgccc gaactgcaag
      961 gcttcaacca attatcccgg ggaggcagcg aggtcgcctt cgggacgatt gggaatgctt
     1021 cctgtgcgga gggcttgttc tgggaatcgc taaatgccat cggcgtgctg ggcgcgccag
     1081 cgttgatcac catctacgat gatgggtacg gcatctcggt gccaaacgag cagcagatca
     1141 tcaagaccaa tatctctgag ctgttacaag gtttccaacg cgatcccgaa cgttgtgggg
     1201 ggtatgacaa ttgtggttac gatatctata cggtgccggg ttgggattac cctcgcctgg
     1261 tgcagaccta ccgcaaagct gaagcaagcg tgcgggatga acatgtgcca gccatcgtgc
     1321 acgtcaacga actgacccag ccgcaaggac actccacgtc gggaagccac gagcgctata
     1381 agaccaatga gcgcctggag tgggagcggg agttcgacgg gctaaagaaa tttcgcgagt
     1441 ggatcattgc tcaggacatt gcgtctgccg atgtgttgga tgagatggat gccgaagacc
     1501 ggggccaggt tgaggaaatc cgcaagcagg cttggcagac gtacatcgcg cccattgagg
     1561 gcgagcgggc tgaattggtt cgactaattg agcaaatggc acagggatcg agccaggcga
     1621 gtgagttgag tgccatccaa cgcaagctaa tcaacacccc caatcccctg cgtcgggatc
     1681 tcatggtggc tacacgcgaa gcgttaatgg cagtgcgcgg cgaacaga
//
LOCUS       DBNHOH010000070         4327 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_451700, whole genome shotgun sequence.
ACCESSION   DBNHOH010000070 DBNHOH010000000
VERSION     DBNHOH010000070.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_451700"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..614
                     /locus_tag="AC16V7_01240"
     CDS             192..614
                     /locus_tag="AC16V7_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020598441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6255769.1"
                     /translation="MKKQHVQLSDVDREYLEELISKGELKAKAYRRAIGLLELDRGKT
                     YTAVSKTLQVTIPTLSGWAAKYQESGLAVLHDKPRSGRPIEIDGNQRAKITALACSEP
                     PEGYAQWSLRLLADKIVELGYCERISHTEVAGILKKTN"
     gene            650..1321
                     /locus_tag="AC16V7_01245"
     CDS             650..1321
                     /locus_tag="AC16V7_01245"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HIR6255770.1"
                     /translation="MDSRFLARMEQILWLYTLPYDSNYPVICFDERPCFLIGDRIEPI
                     VMQTGQVRKEHYAYEKHGSCALFAAIEPLTGFRLAQVHRRRTKKEYTLFCQALAAQFP
                     NAIKIRLVQDNLNTHDVSAFYENLPADEARALADRFEFYFTPKSASWLNMIEIEFSVI
                     ARQCLNQRIPTMELLEQEVLAIVSDRKAKKVKITWQFSLQSARTKLNSHYVKVHSGNA
                     KFKGS"
     gene            1840..2514
                     /locus_tag="AC16V7_01250"
     CDS             1840..2514
                     /locus_tag="AC16V7_01250"
                     /inference="COORDINATES: protein motif:HMM:NF046475.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HIR6255771.1"
                     /translation="MNQSERKITALKAQKRNRSRVSVYLDGEYAFGLARIVAAWLQVG
                     QVITDEKIAELKAQDAGEVAYQQALRLLNYRDRSESEIIRSLKQRQTPEEIITEVIAR
                     LRRNGLLDDQRFAKDWVENRTEFRPRSSRALSYELLEKGVEQETIQQVLESFDEDEAA
                     YLAAKKYVRRIENLEWPEFRQRLLGYLARRGFQYETTRAAIARIQAEFKMNESTQNTL
                     LEEVDR"
     gene            2511..4043
                     /gene="rny"
                     /locus_tag="AC16V7_01255"
     CDS             2511..4043
                     /gene="rny"
                     /locus_tag="AC16V7_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HIR6255772.1"
                     /translation="MNGIWFWIPITLIALLIGGAIGYYLKRILIEKELEKDKAKANLI
                     LEESQKRANELELRAKNQALEIRQTAEAEIARRRGEINREEDRLQKRREEVDMRSDRL
                     EKREQALNKRQSAVDRRTNEIERLHSEQVEELQRISQMSLDEARNQLLSEVEKESRND
                     MARIIRQIEAEAREQGEQRARLIITGAIQRVASEHVSEVTTSVVPLPADEMKGRIIGR
                     NGRNIHAFEQVAGVDVIVDDTPEAVTISCFDPVRREIARRALAKLVMDGRIHPAHIEK
                     VLKNEQKEVERDIVDAGEQATYDAGVAGLHPEVIKTLGRLKYRTSYGQNQLAHAVETA
                     RIAAMIASELGAEVEVARAGALLHDLGKAIDHNMEGTHAQIGADLARRCGVNPKVVNA
                     IASHHHEIDQETVEAAIVEAADAISGARPGARRESLEQYIKRVRALEEIANSFKGVNQ
                     SYALQAGREVRIFVKPEEIDDLDSVRLARDIARNIEDSMQYPGQIKVTVIRETRTIDY
                     AK"
     gene            complement(4172..>4327)
                     /locus_tag="AC16V7_01260"
     CDS             complement(4172..>4327)
                     /locus_tag="AC16V7_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6255773.1"
                     /translation="GRTDLPGGDFNTLIQSIRTQILTLPDDTRLLSGHGPETTVGQER
                     RFNPCLL"
BASE COUNT         1202 a         1026 c         1113 g          986 t
ORIGIN      
        1 caggagactc aacatgaccg gccacggtca gggcgtcaat caagtccaga accgcatcgg
       61 gtcgtttgag caggctctgg tatactgcat ctcggaattg ttggatgagt ttgttgatca
      121 cactcaatac accgtaaagt aagtgatcgt aaaattttga caaattctgc cgaatgtatg
      181 aaaatggcag gatgaaaaaa caacatgtac aactcagtga tgtcgatcga gaatatctcg
      241 aagaattgat cagcaaaggt gaattgaagg ccaaagccta tcgaagagcc atcggattat
      301 tggaattgga tcgaggcaag acctacacag cagtgtcaaa gacattgcag gtaaccatac
      361 caaccctgtc gggctgggca gcgaagtacc aggagagtgg attagcagtg cttcacgaca
      421 aacctcgctc gggtcgaccg attgagattg atggaaacca gcgagcgaag atcacagcct
      481 tggcttgtag tgaaccaccg gaaggttatg cccaatggag cttgcgatta ctggccgata
      541 agatcgtcga gttaggctat tgtgagcgta tttcacatac ggaggtggct gggatactca
      601 aaaaaacgaa ctaaagccgc atctcaaacg cacctggtgt attgggaaac tggacagccg
      661 atttctagcc cgcatggagc agatattatg gctatatact ttgccctatg actccaatta
      721 tcctgtcatt tgcttcgatg aacgaccatg cttcttgatc ggagatcgca ttgagcccat
      781 tgtgatgcaa accggacaag ttcgtaagga acattacgcc tatgaaaaac acggctcctg
      841 cgccttgttt gcagccattg agccactgac aggttttcgg ttagcgcaag tccatcgaag
      901 gcgaaccaag aaggaataca cactcttttg tcaggcttta gcagcccaat ttccaaatgc
      961 gatcaagatc cgtttggttc aagataatct caacacccat gatgtgagcg ccttttatga
     1021 gaaccttccc gcagatgaag cccgtgcttt ggccgatcgt tttgaattct atttcacgcc
     1081 taaatcagcc agttggctga atatgattga aattgagttc tctgtgattg cccggcaatg
     1141 cctcaatcaa cggatcccaa ctatggaact ccttgagcaa gaagtcttgg cgattgtgag
     1201 cgatcggaag gcaaagaaag tcaagattac ctggcaattc tctttacaat cggctcggac
     1261 caagctcaat tcacattatg ttaaggtaca ttcaggtaat gcaaaattca agggttctta
     1321 atttacagtg taataaattt ggggtgaata aaagatttcc cattatagtg aaggctataa
     1381 gcccaacaaa tcttgtgtga ttttctatgc acataatgat ataataccta acaatataga
     1441 ccattaataa atcattggtt gcattaagaa atgtaaccca ggttctcctg ctgatttttg
     1501 atcgatcacg gctttggaat ctaacgcttg aaaaagcttg tctcagaaaa aatgattgtc
     1561 ctggtgtatt ttcgaactgg aataagtgac tataaattta gcgcatgtga ctgcggccgg
     1621 ccttatatcc tgtgccggaa ctcgagacct tatcgccatt gccaatcatt gagatagatg
     1681 tacttgacag gttaataatt tgctgtaaat agacctttgt gtggtctaca tggcacgttt
     1741 cgcctgtcgt gtcatataac tgagatatag aatatccccc acgcagccgc gattgatcaa
     1801 ggtgtgatca cggctgtttt ttattagata cgcgatttga tgaaccaatc ggagcgaaaa
     1861 atcaccgccc taaaagccca aaagcgcaac cgctctcgag ttagtgtcta cctcgacggg
     1921 gaatatgctt ttggccttgc ccgcatcgtt gccgcctggt tacaggtcgg acaggtgata
     1981 accgatgaga agatcgcgga gctcaaggca caagacgctg gcgaggtggc atatcaacaa
     2041 gcgctccgat tgcttaatta tcgcgaccgc tctgaatccg agattatccg cagcctaaag
     2101 caacggcaaa ctccagaaga gatcataacg gaagtgatcg ctcgcttgcg cagaaatgga
     2161 ctgctggacg atcagcgctt tgcgaaagac tgggtggaga accgtactga atttcgtcca
     2221 cgcagtagcc gcgcattgtc gtacgaattg ctagaaaaag gtgttgagca ggaaaccatc
     2281 cagcaagtgc ttgagtcctt cgacgaggat gaggcagcat acctggctgc taagaaatat
     2341 gttcgcagga tagaaaattt ggaatggcct gaattccgtc aaaggttact aggttattta
     2401 gcccgccgtg gttttcaata tgaaaccacc cgcgctgcca tcgcacgtat tcaggctgaa
     2461 tttaagatga atgaatctac acagaatacc ttacttgaag aggttgatag atgaatggaa
     2521 tatggttttg gattccgatt accctgattg ctttgctgat tggtggtgca atcggttact
     2581 atttgaagcg aatcctgatt gaaaaagaac tcgaaaagga taaagctaaa gcaaatctca
     2641 tactagaaga atcccaaaag cgcgccaacg agttggagct acgcgccaag aatcaagctc
     2701 tggagattcg ccaaaccgcc gaagctgaaa ttgcacgccg tagaggtgag atcaatcgcg
     2761 aggaagaccg gttgcaaaaa cgtcgcgaag aagtggacat gcgatccgac cggctggaga
     2821 agcgtgagca ggcactcaac aagcgccaga gcgctgtgga tcgacggacc aatgagatag
     2881 agcggttgca ttccgagcag gtagaagaat tgcagcgcat ctctcaaatg agcctagacg
     2941 aagcgcgcaa ccagctgctt tccgaggtgg aaaaagaatc tcgtaacgac atggctcgaa
     3001 ttattcgcca gatcgaagcc gaagcgcgcg agcaaggcga acaacgagcc cgcctgatca
     3061 tcaccggtgc tatccaacgc gtggcctctg agcatgtttc cgaagtgacc acatcggttg
     3121 tgcctctgcc tgccgacgag atgaaaggac gcattatcgg gcgcaacggg cgcaatatcc
     3181 acgccttcga gcaggtcgct ggagttgatg tcatcgtgga tgacacgccc gaggcagtga
     3241 caatttcctg ctttgatccg gtgcggcgcg agattgccag gcgggcacta gccaaactgg
     3301 ttatggacgg tcggattcac cccgcccata tcgaaaaagt cttgaagaat gagcagaaag
     3361 aagtcgaacg agatattgtc gatgccggag agcaggccac ttacgacgct ggtgtggcgg
     3421 gtttgcatcc agaggtcatc aaaaccctgg gacgtttaaa ataccgtact tcctatgggc
     3481 agaaccagct cgcccacgcg gtggagactg cccgcatcgc cgccatgatc gcgtctgagc
     3541 tgggcgccga ggtggaagtc gccagggcag gcgctcttct ccacgacctg ggtaaagcga
     3601 tagatcataa tatggaaggc actcacgctc aaattggcgc tgatttagca cgccgctgcg
     3661 gtgtcaaccc gaaagttgtc aatgctatcg cctcccatca ccatgagatc gaccaagaga
     3721 ccgttgaagc tgccattgtc gaggcagcag atgcgatctc tggagctcgg cctggagcgc
     3781 ggcgagagag cctggagcaa tacattaaac gcgtgcgtgc cctggaagaa atcgccaact
     3841 ccttcaaagg cgtcaaccag agctatgccc tgcaggcggg tcgtgaggtg cgcatctttg
     3901 tcaagcccga ggagatagac gacctcgact cggtgcgcct agcgcgcgac atcgcccgca
     3961 acattgaaga ttccatgcag tatcccgggc aaatcaaagt gacggtcatc cgagagaccc
     4021 gcacgatcga ttacgcaaag tagagaccgc tgcgcaggtc atccagcaaa agaataataa
     4081 gctgtccaaa aaatatttgg atggcttgga acgcaaacca gagtaggtcg ggtgccatac
     4141 ctgacctact tttctttcac ctaagaaatc atcacaacaa gcagggattg aagcgccgct
     4201 cctgcccaac ggtcgtttca ggtccatgcc ctgaaagcag gcgcgtgtcg tctggcaggg
     4261 tgagtatctg ggtccggatg ctttggataa gcgtattaaa atcaccccct ggcaaatcag
     4321 tgcggcc
//
LOCUS       DBNHOH010000071         1621 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1733825, whole genome shotgun sequence.
ACCESSION   DBNHOH010000071 DBNHOH010000000
VERSION     DBNHOH010000071.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1733825"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="AC16V7_01265"
     CDS             <1..455
                     /locus_tag="AC16V7_01265"
                     /inference="COORDINATES: protein motif:HMM:NF014679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR6255774.1"
                     /translation="AALSNKPFSDIVNLVKEMIPITRMIQTADTLKYLYMGGRIGKAK
                     HLVGSMLNIKPLLSMENGIIVPVGTARSRKRAYQMMVEKVEAAVGFMGKIKVAYVHAA
                     AVEEAEKIRSLVEERLTCVESLIAELSPALGVHTGPGTAGLCYFPVNT"
     gene            489..>1621
                     /locus_tag="AC16V7_01270"
     CDS             489..>1621
                     /locus_tag="AC16V7_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255775.1"
                     /translation="MYLEELVEVAVGLILVYILLSLAVSNIQEWISRWINNRSAFLET
                     TLREMLAETLNTPEGGRVTDRKYIPENKSGITEKVYNHPMIKSLYQGKGRPSYIPADK
                     FVLALFDIVVTAGSDGSTIEKALQKIKSYKERVPEASLAGLEAGIDELARQAKAARDD
                     PVKLAALQQDIENFAQEFKNASGFDVKPIFEGFLQAELPVKEEEVIQALKRGAAMLTI
                     ENLQLKQTLDDLVYQAEVYIKGAEGKLAQIRANAEKWFDDTMDRAAGWYKRSAQFWAF
                     YLGLILALFFNLDTVEIASRLWVQPTLRHSLIRAAETYQLEDINAQDEAPNPVETITR
                     LQSSLTGLHLPVGWAFQSLGPDLFNPAVDRCTLFPRSTSEVRAG"
BASE COUNT          435 a          386 c          419 g          381 t
ORIGIN      
        1 gcgctgcttt gagtaataag cccttttctg atatcgtcaa cctggttaaa gagatgatcc
       61 ctatcacgcg catgatccaa accgctgaca cgcttaaata cctgtacatg ggcggtcgga
      121 tcgggaaggc taaacatctt gtagggagca tgctgaatat caagcccttg ctcagcatgg
      181 aaaatgggat tattgtgccg gtcgggacag cacgcagccg gaagcgcgct taccaaatga
      241 tggtggaaaa agttgaggcg gcggttggct ttatgggaaa gatcaaggta gcctatgtgc
      301 atgctgctgc ggtcgaagag gcagaaaaga tcaggtcgct agttgaagaa cgtttgactt
      361 gcgtagaatc cctaatcgct gagctttccc ccgccctggg tgttcacact ggtcctggta
      421 cagcaggtct atgttatttt cccgtcaaca catagttaaa tacttccgaa gttacaaaag
      481 gaggagcaat gtatttggaa gagctggttg aagtcgccgt aggtctcatc ctggtataca
      541 tcttgttgag cctcgcagtt tcaaacatcc aggagtggat ctctcggtgg attaataatc
      601 gctctgcatt tctcgagact accctacgag agatgctagc ggaaacctta aacacgcccg
      661 aaggtggtcg cgtcacggat agaaaataca tcccagaaaa taaatcaggg ataacagaga
      721 aggtgtacaa ccacccaatg atcaaatccc tatatcaagg taaagggcga ccctcctaca
      781 tccctgccga caaattcgtg ttagccttgt ttgatatcgt tgtgactgcc ggcagcgacg
      841 gctccacgat tgagaaagca ctgcaaaaga ttaagagcta caaagagaga gtccctgaag
      901 catcgctggc tggtttggag gctggcatag atgagttggc caggcaagcc aaggctgctc
      961 gagacgatcc agtgaaacta gctgctttgc aacaggacat tgaaaatttc gcccaagagt
     1021 ttaaaaatgc cagtgggttt gatgtaaaac caatttttga gggcttcctg caagccgagc
     1081 tgccagtaaa agaggaggag gtgatccaag ctctgaagcg tggcgcggct atgttaacaa
     1141 tagaaaatct acagctaaag caaaccctag acgacctggt ttaccaagct gaggtataca
     1201 ttaaaggagc agagggtaaa ctagcccaga tccgcgccaa tgcagaaaag tggtttgatg
     1261 acacgatgga ccgcgcggca ggctggtata agcgcagcgc tcaattttgg gctttttacc
     1321 tggggctaat tttagcgctt ttcttcaacc tggataccgt cgaaatcgcc tcccgccttt
     1381 gggttcaacc cacactacgt cacagtctga ttcgagctgc tgaaacctat caattagaag
     1441 atatcaatgc ccaagatgag gcgcccaatc cagtagagac aatcacccgc ttgcagagtt
     1501 cattgacagg cttgcacttg cctgtgggat gggccttcca gtccctagga cccgatttat
     1561 tcaacccggc tgttgaccgg tgtactctgt tcccccgctc gacctccgag gttcgtgctg
     1621 g
//
LOCUS       DBNHOH010000072         2842 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_259674, whole genome shotgun sequence.
ACCESSION   DBNHOH010000072 DBNHOH010000000
VERSION     DBNHOH010000072.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_259674"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="AC16V7_01275"
     CDS             <1..686
                     /locus_tag="AC16V7_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6255776.1"
                     /translation="GVDVAGGIPWIEYTDEDIREHIKVVFDLAVEFDKDVSMLVDDAG
                     DPGLRSLEWMALETIRRGWHGRSLAHHARAMAMYPTPYFQKVAALLKKAQMGVVTDPH
                     TGPLHARVRELLQEDVLVCLGQDDISDAYYPYGRNNMLEVAFLASHLLWMTTASDMEI
                     LYDMITRRAAQSMGLRDFRLKVGAPAHLVVLGQPNVLEALRYHQAPLHVISHGELVDP
                     ARVDAILNQ"
     gene            778..>2842
                     /locus_tag="AC16V7_01280"
     CDS             778..>2842
                     /locus_tag="AC16V7_01280"
                     /inference="COORDINATES: protein motif:HMM:NF014758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HIR6255777.1"
                     /translation="MLKPDETRLVVVRFTLNGDEVEARVPGEMTVLRFLRDEMGMLGV
                     KDGCSTGHCGACMVIVGGKATRACLVKMAKIDQARVETIEGLAKDGALHPLQQAFIEK
                     GAVQCGFCTPGIIMTAKALLDSSPRPSRTEIKKTLTQNRNLCRCTGYIKIFEAIELAA
                     ERMANGRSQSQALADDDPTTGKSLIDQIAADMVTGENTFGDDLAMEGMLHGKILWSAH
                     PHARILSIDTSEAEVMQGVVAVITAKDIPGKNQAGVVVRDQPAIAAEKVRYIGDSLAA
                     VIAETVEIAEAAVGKIRVEYEVLPGVFSPDEASQPGAPKVHEKGNLLHQAEIVRGDVE
                     DAFERCAVVVEDTYTTPFIEHAFLEPESGISYVDDDGSLVLKIGTQTAFDDRAQLSEI
                     LDMPEEKIRVIQIPPGGAFGGKEDMILQQYLALGALKTRQPVKMVLTREESLRVHVKR
                     HPARMRFKTGADNRGRVLALEADITLDTGVYASLGIDVLENTVVFAAGPYFIPNLRIR
                     GQAWHTNNVLAGAMRGFGVNQVAFALEQQMDAMARALNMDAFEFRLLNALDVGLPTAA
                     DHVLEEGVVSIKQTIEAARQAYQGLSRPSSNGKKIGVGVACAVKNIGFGHAIPEDAGA
                     EVELDACGNVQVRASQHEYGQGARMGLIKLASRTLGIPAEHIQVLVPDTHITPYTGPT
                     TASRQT"
BASE COUNT          624 a          772 c          883 g          563 t
ORIGIN      
        1 taggagtaga tgtggctggg ggcatcccct ggatcgaata cacagatgaa gacatccggg
       61 agcacatcaa ggtggtcttc gatttagccg tggagtttga taaagatgtc tccatgctgg
      121 tggacgatgc cggcgaccct gggctgcggt cgctggagtg gatggccttg gaaacgatcc
      181 gccgcggctg gcacggcagg tcgctggcac accatgcgcg cgccatggcg atgtatccca
      241 caccgtattt tcaaaaagta gctgcgttat tgaagaaagc ccagatgggc gtggtcaccg
      301 atccgcacac cggcccgctg catgcaaggg tcagggaact cctgcaggag gatgtcttgg
      361 tatgccttgg gcaggatgat atctcggatg cctattaccc ctacggacgc aacaacatgc
      421 tggaggtggc tttcctggct tcgcacctgc tgtggatgac caccgcctca gatatggaga
      481 tcctgtacga tatgatcacg cgccgcgcgg cacagtccat ggggttgaga gatttccgct
      541 tgaaggtcgg cgccccagct cacttggtcg tcctggggca gcccaatgtc ctggaagcgc
      601 tgcgctatca ccaggcgcct ctgcatgtga tcagccatgg cgagctggtc gatcctgcca
      661 gagtggatgc catcctgaat caatgaggct ggcgtggttt ctctgaaaag ctgctcttat
      721 gcggagtatt tccttgccac ccatcactca atctgctcaa tccagacaat gtgacctatg
      781 ctaaaaccag acgaaaccag gctagttgtt gttcgcttta ccctgaatgg ggacgaggtc
      841 gaggctcggg ttccgggcga gatgaccgtc ttgcgcttcc tgcgcgatga gatgggcatg
      901 ctgggcgtca aagatggctg ctcgacaggc cactgcggcg cttgtatggt gatcgtgggc
      961 ggtaaagcca cccgagcgtg cctggtgaag atggccaaaa tcgaccaagc gcgggtggag
     1021 acgatcgagg gactggcgaa ggatggcgcg ctccatcccc ttcagcaggc attcatcgaa
     1081 aagggggctg tgcagtgcgg attttgcacg ccaggcatca tcatgactgc caaagccctg
     1141 ctggattcca gcccccgtcc ttcacgtacc gagatcaaga aaaccctcac ccaaaaccgc
     1201 aacctgtgcc gctgtacggg ctacatcaaa atcttcgaag ccatcgagct ggctgcagag
     1261 cggatggcta atggacgctc tcaatctcag gcattggcag acgacgaccc tacaaccgga
     1321 aaatccttga tcgaccagat tgcagcagat atggtcacag gagagaacac gttcggtgat
     1381 gatctggcca tggaaggaat gctgcacggc aaaatcctgt ggtctgcaca cccacacgcg
     1441 cgcatcttga gcatcgacac gagtgaagcc gaagtgatgc agggcgttgt ggcagtgatc
     1501 acggccaaag atatccctgg aaaaaaccaa gccggggtag tggtgcgaga ccagccagcc
     1561 atcgccgctg aaaaggtgcg ctacattggt gattcgctgg cagcggtaat tgccgagacg
     1621 gtagagatcg cagaagcggc cgtgggtaaa atccgggtcg agtatgaggt cctgccgggc
     1681 gtattttcgc cggatgaagc ctcccaaccc ggtgcgccga aggtgcatga aaaaggcaac
     1741 ctgctgcacc aggctgagat cgtgcggggg gatgtggagg atgcctttga acgctgcgcg
     1801 gtagtcgtcg aagataccta taccacaccc ttcatcgaac atgccttcct ggagcctgag
     1861 tcgggaatca gctatgtgga tgacgatggc agcctggtgc tcaagatcgg cacgcagaca
     1921 gcctttgatg accgcgcaca attgtctgaa atcctggata tgccggagga aaaaatacgc
     1981 gtgatccaga tcccgcctgg cggggcgttt gggggcaagg aagacatgat cctgcagcaa
     2041 tacctggcac tgggcgccct gaagaccagg cagccggtca aaatggtgct aacccgagaa
     2101 gagtcattgc gtgtgcacgt caagcgccac ccggcgcgca tgcgcttcaa aactggcgca
     2161 gacaatcgcg ggcgtgtcct ggctctggag gcggatatca ccctggacac cggcgtgtac
     2221 gcttcacttg ggattgacgt gctcgagaac acagtcgtat ttgcggcggg accgtacttt
     2281 atacccaacc tcagaatccg cggccaggcg tggcacacca acaatgtttt ggctggggcg
     2341 atgcgcggct tcggcgttaa tcaagtggca tttgcgctgg aacagcagat ggatgccatg
     2401 gcccgcgccc tgaacatgga cgccttcgaa tttcgcctgt tgaacgctct ggatgtgggc
     2461 ctgccgaccg ccgccgatca tgtgttggag gaaggagtcg tctcgatcaa gcagaccatc
     2521 gaggctgctc gccaggccta tcaagggctc agccggcctt ccagcaatgg caagaagatt
     2581 ggggtgggcg tggcctgtgc ggtgaaaaac attgggtttg ggcacgccat tcctgaggat
     2641 gctggggctg aggtggagct ggacgcctgc ggcaatgtgc aggtgcgcgc tagccagcat
     2701 gagtacggac agggcgcgcg catgggcttg atcaagctgg cttcgcgtac gttaggtata
     2761 ccggcagagc acatccaggt gctggtacct gatacccata ttacgcccta caccgggccg
     2821 acgactgcct cgcgacagac tt
//
LOCUS       DBNHOH010000073         1599 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_772455, whole genome shotgun sequence.
ACCESSION   DBNHOH010000073 DBNHOH010000000
VERSION     DBNHOH010000073.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_772455"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="AC16V7_01285"
     CDS             <1..611
                     /locus_tag="AC16V7_01285"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6255778.1"
                     /translation="PGEFLGIIGKSGAGKTTLINILTGVDRLTSGEVWMDELPIHRLD
                     ENQLATWRGRNVGIIYQSFHLMPNLSLLQNVILPMDVSGLFRSGESEQRALELLQMVE
                     LEDHARKPPSAISGGQQQRVAIARALANDPPIIVADEPTGRLDSSTAETIFQIFTRLM
                     EEGKTILMVTHDQSLARRMTRTMHLVNGGIAHQHGMQLRGRG"
     gene            629..>1599
                     /locus_tag="AC16V7_01290"
     CDS             629..>1599
                     /locus_tag="AC16V7_01290"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6255779.1"
                     /translation="MKGTNQPNYLPLPNRCSDDALIEMAQIVKTFKNAAGEFTALNEV
                     TACFYEGEFVSVVGKSGSGKSTLVNMITGIDHPTSGTVRIGDAYVHALSESDMSIWRG
                     KNLGIVFQFFQLLPTMSLLENVMLPMYLSGQYSPAEREERALSLLKLVGLKGLDDKLP
                     AAVSGGQQQCAAIARALANDPPILIADEPTGNLDSRTAEEIYDLFAGLIDQGKTILMV
                     THDISLSERTTRTLLISDGELINPWVASALPHLGHSQLLWLTHNLKARQLPAGAIVPD
                     LHSQGDWLILVAGGQLEVLDDRGGHREQPLMRLGAGNALSSLDLQASG"
BASE COUNT          386 a          409 c          462 g          342 t
ORIGIN      
        1 atccaggtga attcctaggt attatcggca aatcaggggc aggaaaaaca accctaatca
       61 acatactgac aggcgtagat cggctgacca gcggcgaagt gtggatggat gaattgccta
      121 ttcaccgcct ggatgaaaac cagctggcca catggagggg gcggaatgta ggcattattt
      181 accaatcctt ccacttgatg cccaacctat cccttttaca gaacgtgatc ttgcctatgg
      241 atgtcagtgg actctttcgc tcaggcgaga gcgagcaacg ggcgctggag ttattgcaga
      301 tggttgagct ggaggaccat gcacgcaaac cgccatcggc tatatctggc gggcagcagc
      361 aacgcgtggc aattgctcga gcattagcca atgatccgcc aattattgtg gctgacgagc
      421 ccactggtcg actggattct tccacggcag agacgatttt tcaaatcttc acccgattga
      481 tggaagaggg caagacaatc ttgatggtga cgcacgacca gagtctagcc cgacgtatga
      541 cgcgcaccat gcatttggtg aatggtggta ttgcacatca gcatggtatg caacttagag
      601 gtagaggtta aaggaataag tgaatagcgt gaaaggaacc aaccagccaa actacttgcc
      661 gcttcctaac cggtgcagcg acgatgccct tattgagatg gcgcagattg tcaagacctt
      721 taagaacgcg gctggggaat ttactgccct caatgaggta actgcctgct tttatgaggg
      781 cgaattcgtc agtgtggtag gtaaatcagg cagcgggaag tcgacactag tgaatatgat
      841 cacggggatt gatcacccaa cctcgggtac ggtgcggatt ggcgatgcat atgtgcacgc
      901 cctgagcgaa agcgacatgt cgatctggcg gggtaaaaat ctgggaattg tctttcagtt
      961 tttccaattg ctgccgacta tgtctctatt agaaaacgtc atgctgccca tgtatcttag
     1021 cggccagtac tcgccagccg agcgggagga gcgcgccctg agcttgctca aacttgtcgg
     1081 gttgaaaggg ctggatgata aattgccggc ggctgtatca ggcggtcagc aacagtgtgc
     1141 ggcaattgcc cgggctttgg cgaatgaccc acccatcctg atcgctgatg aacctaccgg
     1201 caacctggac tcgcggacag ccgaagagat ctacgacctc ttcgccgggc tgatcgacca
     1261 gggaaaaacc atcctgatgg taacccacga tatcagcctc tcagagcgca cgacacgcac
     1321 gttgctcatc tccgatggcg agctgatcaa cccctgggtg gcgagcgcgc tgccacatct
     1381 cgggcacagc cagttgctat ggctgaccca caatctgaag gcgcgccaac tcccagctgg
     1441 tgccatcgtc ccagatctgc atagccaggg ggattggctg atcttggtcg cgggtgggca
     1501 gctggaggtt ctggatgatc gtggggggca ccgcgaacaa cccctcatga gactaggagc
     1561 agggaacgct ttgagcagcc tagacctgca ggcgtccgg
//
LOCUS       DBNHOH010000074         3261 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_195797, whole genome shotgun sequence.
ACCESSION   DBNHOH010000074 DBNHOH010000000
VERSION     DBNHOH010000074.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_195797"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..832
                     /locus_tag="AC16V7_01295"
     CDS             104..832
                     /locus_tag="AC16V7_01295"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR6255780.1"
                     /translation="MSEQENIRIAEKFFQAINAHDLSQNSDHYAPDYLYEAPGVPGQF
                     NQEQSQAYTQGFLDAFPDLHFEIKHKIAQGDYVVINWRATGIHTGPLTTPTGNQLPPT
                     GKKAVVPGSTTYEFKDGKVVRSWVHWDMVTLLAQLGLMPGVEPSQPAQENIGTYRVVD
                     GEKANGHWQVGVNAKGKPVLGLPPVNERMQWLPGQLVQVHLPEADVDGPTKFWVVEGK
                     AYKGNKFNNPQVYVKAGDGELIEA"
     gene            complement(948..1430)
                     /locus_tag="AC16V7_01300"
     CDS             complement(948..1430)
                     /locus_tag="AC16V7_01300"
                     /inference="COORDINATES: protein motif:HMM:NF042610.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6789 family protein"
                     /protein_id="HIR6255781.1"
                     /translation="MTNLSSFSQDYPKAGDAAVNGLFGGLVAGVGMAIALVTTGLVTG
                     SSGASTLARFDPSNSSQALTGTLAHLAVSGTYGLIFGLIWSTATRLMRMHPSLRQTIL
                     AGGGYGLMLWLAAHFVLLPGTQSPMRELAIWQFLSAHLVYGLLLGWRVGRSGQQDLSI
                     "
     gene            1713..2087
                     /locus_tag="AC16V7_01305"
     CDS             1713..2087
                     /locus_tag="AC16V7_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007050103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsmA family protein"
                     /protein_id="HIR6255782.1"
                     /translation="MPLNMQLSVILITLALVFYTVGVWSERWQGRLKGWHLVFFWAGL
                     VCDASGTGIMFDMADADLFSLHGLTGLLAIVLMFVHAVWASLVLYRKDERAITNFHRF
                     SVVVWLIWLVPYFNGFFMGMGE"
     gene            complement(2109..3128)
                     /locus_tag="AC16V7_01310"
     CDS             complement(2109..3128)
                     /locus_tag="AC16V7_01310"
                     /inference="COORDINATES: protein motif:HMM:NF014420.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HIR6255783.1"
                     /translation="MAIEYQKGNSLLHKLDARTKLLLFAGSTVLAVIIVDPIIMAALF
                     FLLYLMGTKAVDRKNLNRNLRVLVVIFFTFSLFQIFFFTPSDAHFLFFLIPFKNWVPV
                     TVEGIIRGVAVFFRFFSVVLAVHLMLYTTPPVDMVLAITKRERNRSLLQHIGLMALFG
                     LFFLVIAFLVMPQTIQRIPLPLILRGLLVFAISFVVAVVIEKILSNGLPPEMGVALTL
                     GFATVGILSQQTQKITDAQKARGYDVQPKNLIRRVQVLTALLLPIFLATLERSQDIAI
                     AILARGFDYNIAARTYRRQLSFTKIDYIFQASIILLLIGGLILNYYGVGNPTEQLVMK
                     ILGYQ"
BASE COUNT          832 a          795 c          865 g          769 t
ORIGIN      
        1 ggtctaactg gtaactgcct gtgctctcgc catatagtaa tcgtctagcc tgagcatagg
       61 tgatccttca gcagtattta aatagcgata ggagatttta accatgtcag aacaagagaa
      121 tattcgcatc gctgagaaat tctttcaagc catcaatgcc cacgatcttt cccagaactc
      181 ggatcactat gcgcccgact acctttacga ggcccctggt gtccccggcc aatttaacca
      241 ggagcagtct caggcctata cccagggctt tttggatgcg tttcccgatt tgcactttga
      301 gatcaaacac aagattgccc agggcgatta cgtagtcatc aactggagag ctactggaat
      361 ccacactggc ccgctcacca ctccaaccgg taaccaactt cctcccacgg gcaaaaaggc
      421 tgttgtccca ggcagcacga cctatgagtt taaagacggg aaggttgtcc gctcgtgggt
      481 ccattgggac atggtcaccc tgctggcaca gctcggattg atgccaggtg tcgagccgtc
      541 acagccagcg caggaaaaca tcgggacgta ccgtgtggtg gatggcgaga aggcaaatgg
      601 gcactggcaa gttggcgtca acgccaaggg taagccggtc ttagggctcc cgccagtcaa
      661 cgagcgcatg cagtggttgc ccggacagct tgtacaggtg cacctgccgg aagctgacgt
      721 agatggcccc acgaaattct gggtggtaga ggggaaggct tataagggca ataagttcaa
      781 taacccgcaa gtgtacgtca aagcgggcga tggtgagctc atagaggcat agccctaaga
      841 aatattggga agtctgctct ggtattcttt ggaagaggta cctggcgact ttgcgctcct
      901 tccaggggcg aatgccagcg agcctaatct gcctgctttc aattgcttta tatgctcaaa
      961 tcctgctggc cgctacgccc aactcgccag cccaataata gcccataaac caggtgagct
     1021 gagaggaact gccagatggc gagttcgcgc attggcgact gggtgcccgg cagcagcacg
     1081 aaatgcgccg ccagccagag catcaatcca tatcctccac cagcaaggat agtttgcctg
     1141 agggatggat gcattcgcat caggcgagtg gctgtgctcc agatgaggcc aaaaatcagc
     1201 ccgtaagtgc ccgagacagc caggtgggcc agtgttccgg tgagcgcttg actgctgttt
     1261 gatgggtcga agcgcgccag cgtagaggca ccagaagaac cggtgacaag accagtcgtc
     1321 actagggcaa ttgccatacc cactccagcg accagcccgc caaacaatcc attgacggcg
     1381 gcatcgccag ctttggggta atcttgagaa aagctgctga gatttgtcat tttcgaatcc
     1441 tctagtctaa gatagcgata tcaaacattg atggatggcc gctaactgcc tttggttatc
     1501 aaggtaacta tattgatatc ttagataaac tttatcaaat agacgttcgg aattcgttac
     1561 gaagagcgtt acgatctctc ccaaaaattg tggtttttga agcgctgtga tcgtctcaat
     1621 tatccatgtg aactgcacct caccaaactc acgccgggga taagcgggta caattaactc
     1681 ttgttgctca ttgctaatta caggagagtc ttatgccgct gaacatgcag ctttcggtaa
     1741 ttttgatcac gcttgcgctg gttttttaca ccgtcggcgt gtggagcgaa cgctggcaag
     1801 gtcgattgaa gggctggcac ctggttttct tttgggcggg tctggtttgt gatgccagcg
     1861 gaactggtat catgtttgat atggcagatg ccgatttgtt cagcctccat gggctaacag
     1921 ggctgctagc aattgtgctg atgttcgtcc acgctgtttg ggctagtctt gtgctctacc
     1981 gtaaagatga acgcgccatc accaatttcc accgcttcag cgtcgtggtc tggttgattt
     2041 ggctggtgcc ctactttaac ggattcttta tgggtatggg ggagtgagtt gatagaagga
     2101 tagcctggtt attggtatcc cagtattttc atcaccagct gttcggttgg atttcccaca
     2161 ccataatagt ttaagatcaa tcctccaatg agtaacaaga taatggaggc ttgaaagatg
     2221 tagtcgattt tggtgaagga cagctgccgg cgataggtgc gcgcggcgat attgtagtca
     2281 aagccacgag ccagaattgc aatcgcaata tcctgcgatc gctcgagagt agccagaaat
     2341 atggggagca gcagggcggt gagcacctga actcggcgaa tgagattctt cggttgcaca
     2401 tcatagccac gcgctttctg agcatcggtg attttttgcg tttgctgaga gagaataccc
     2461 accgtagcga agcccagggt caatgcaacc cccatttctg gcggaagccc gttgcttaat
     2521 attttttcga taacaactgc gaccacaaac gagatggcaa agacgagcaa gccgcgtaaa
     2581 attagtggta atgggattcg ttggattgtt tgcggcataa ccaggaacgc gatgaccaaa
     2641 aagaacaatc cgaagagagc catgagccca atgtgttgaa gtaaactgcg attgcgttct
     2701 cgctttgtga tggctaagac catgtcaact ggcggggtgg tatacaacat caggtgaacc
     2761 gccaaaacca cgctgaaaaa gcggaagaat acggctacac cccggatgat gccctccaca
     2821 gtcactggca cccaattctt aaagggaata aggaaaaaga ggaaatgagc atcgctcggc
     2881 gtgaagaaaa atatctgaaa caaagagaat gtaaaaaaga tgactaccag cacgcgcagg
     2941 ttgcgattca ggtttttccg gtctacagcc tttgtgccca tcaggtaaag caaaaaaaac
     3001 aaggcagcca tgataatggg atcaacgatg atcactgcca gcaccgttga tcctgcgaac
     3061 aacaagagct tggtcctggc atccagcttg tgcagcaatg agtttccctt ctggtactca
     3121 attgccattg ctcgcctcca gcaaatgtct gagcgattct tcgacatcct ccatggaaac
     3181 caaccagggg ggagtgccat gtttgcttaa gcgaatcgcc agctttgtcg tctcgggtag
     3241 atcaagcgag tttgcctgca t
//
LOCUS       DBNHOH010000075         4535 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_387907, whole genome shotgun sequence.
ACCESSION   DBNHOH010000075 DBNHOH010000000
VERSION     DBNHOH010000075.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_387907"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="AC16V7_01315"
     CDS             <1..260
                     /locus_tag="AC16V7_01315"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559685.1"
                     /note="catalyzes the formation of 5-phospho-alpha-D-ribose
                     1-phosphate from D-ribose 5-phosphate and ATP; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIR6255784.1"
                     /translation="VNLVKAHGARNVYLIFVHAVLSPPAMQRIAQLPLKEIITTDTIP
                     IPSDQRALIQDRLTILSVAPLLGEVILRAHEGRSVGELFDE"
     gene            264..1100
                     /gene="mutM"
                     /locus_tag="AC16V7_01320"
     CDS             264..1100
                     /gene="mutM"
                     /locus_tag="AC16V7_01320"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HIR6255785.1"
                     /translation="MPELPEVETIAANLREGNPNQPSLIGKSIQQAHLLWERTLATPS
                     ASEFYSWIPGQSILDIGRRGKFIVFELSRHTLLIHLRMSGDLLVEPVDAVIAPHHRLV
                     IDLDGGIRLAFNDARKFGRVWLTENPSGVLAALGPEPLDESFRPLDLHQRLQAHRRQL
                     KPLLLDQSFLAGLGNIYADEALHLAGIHPLTQSNMLSLEKSERLWQGIREVLQEGILR
                     NGASIDWVYRGGDFQNYFRVYQRTGNPCYNCGTPIERLVVGQRGTHFCPNCQPFDGRR
                     HS"
     gene            1097..1636
                     /locus_tag="AC16V7_01325"
     CDS             1097..1636
                     /locus_tag="AC16V7_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255786.1"
                     /translation="MNANIMLIVVGLIGIAIGFALGVALMSLRSPQAEEDEKEPPPLQ
                     KRIQTPPPKAPPTVDVAPRPVSKPTPSLEDSPPPQQRPTIKPANVLARVLQPDLSAAQ
                     APPKSIAAQIDEILQEMLEDSPLDSRGIRLMEFPNRGLVVMVGLDQYEGVDAVPDEDI
                     RRLIRSAVHEWERRNTPSS"
     gene            complement(1863..2891)
                     /gene="add"
                     /locus_tag="AC16V7_01330"
     CDS             complement(1863..2891)
                     /gene="add"
                     /locus_tag="AC16V7_01330"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HIR6255787.1"
                     /translation="MRPFEFYQSLPKVDLHRHLEGSLRFETVRDIARSHNLDLPGASQ
                     LRSLVQIQKDEPLTFENFLSKFATLRLLYRSPEIITRITREAIADAAADNVRYLELRF
                     TPVALSKSQGFSLAQVIDWVIEGTRQGEAESGLTTRLIASVNRHEGPTLAAQVADLAV
                     ERRDAGIVGLDLAGSEPGYPAEPFASIFQHAKESGLRIAIHAGEWDHGDNVAEAIEKL
                     DADRIGHGIRVLESPTAIELAQKNQTPFEVCITSNYQSGAVTDLNSHPVAQMLAKGLN
                     VTINTDDPSISQIDLSHEYHLFCETLNFPLEVLRARVLAAAKASFLPESERSKLVQSI
                     DQEFQAHL"
     gene            complement(2970..>4535)
                     /locus_tag="AC16V7_01335"
     CDS             complement(2970..>4535)
                     /locus_tag="AC16V7_01335"
                     /inference="COORDINATES: protein motif:HMM:NF019155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255788.1"
                     /translation="QRNRIFLKDDLSEDVTEMLRTEINQRIPEALQDPEGPWKLLAWM
                     EQIQPPLQFGSQFYPSYMLRLILEHILDGQSGGLSPQAAIQALLEIARQAIQAEEDHL
                     VNAVDMLLEQARERLESQIAERDETLDIFFEGLSLADETEQRSLRQIADELSTVVRLP
                     IRLPSEQLRLLRDDPQEAKDLVYEQLMGYLSAQAVTRLVGSVERRLEESLNIDMNQLP
                     AENWDDLSDQVLSAIQLAFEGRRERYLGEGGQIARELENAIGRADGKLKSNALLQLLM
                     LMPQGARAAFDKKTHRRVYHRTTRFSYVYHAASYLEGREPEAIADDVLKHLELAQAAI
                     RQAWGINEWNRIAASSIQDLDTTTQKGLRSVLSEEDYLSVHQRPLQGLPGEKVERVVN
                     ELGRQALTEIYRQLLLGIITELWVDYLTQMEALRVSIGLEAYAQRDPLVQYKNRAFGL
                     YQDLLGNMRSGVVGRMFTYRPRDLSAVQTTVSRVEAPEVEAVVSGNGPQSAPQIEKET
                     RGGKPKKRRRRRR"
BASE COUNT          947 a         1250 c         1264 g         1074 t
ORIGIN      
        1 cggtcaatct ggttaaagct catggggcga ggaatgtcta cttgattttt gtccacgcgg
       61 tgctttcccc gccagccatg cagcgcattg cccaactgcc tttaaaggag atcatcacca
      121 ccgataccat ccctattccc tcggatcaga gagcactgat ccaagatcgc ttgacaattc
      181 tctctgtggc gcccctgttg ggggaggtca tcctgcgcgc ccatgaaggg cgtagtgtgg
      241 gcgagctatt tgacgagtga gacatgcccg aattgcccga agttgagacg atcgccgcga
      301 atttgcggga gggtaaccca aaccaaccat ccttaatcgg gaagagcata caacaggcac
      361 acttgctgtg ggagcgcacc cttgctaccc cttcggcaag cgaattctat tcctggatac
      421 ctggacaatc tatccttgat attggccgcc gaggcaaatt tatcgtcttt gagctgagcc
      481 gccacaccct gctgatccac ctgcggatga gtggcgacct gctggtggaa ccagtcgatg
      541 ctgttatagc gccccatcac cggctcgtga ttgatctcga tggcggtatc cggctagctt
      601 tcaacgatgc acgtaagttt gggcgcgtct ggctgacaga gaaccccagc ggcgtattgg
      661 ctgctctggg tccagaacca ttggatgaat ctttccgccc acttgatttg caccagcggt
      721 tgcaggctca tcgccgccaa ttaaagcctt tgttgcttga ccaaagcttc ctggctggtt
      781 tgggcaatat ctacgccgat gaagccctgc atttagctgg cattcatcca ttaacccagt
      841 caaacatgct ttccctggag aaaagcgagc ggctttggca gggcatccgt gaggtgctgc
      901 aggagggcat cctgcgcaat ggcgccagca ttgactgggt gtaccgggga ggggactttc
      961 agaattactt ccgggtgtac cagcgcacgg gaaatccctg ttacaattgc ggtactccca
     1021 ttgaacgctt ggttgtgggg caacgcggca cacatttttg ccccaattgt cagccttttg
     1081 acggcaggag gcattcatga acgcgaacat tatgctgatt gttgttggcc tgatcggaat
     1141 agcgatcggc tttgctctgg gcgtggcctt gatgagtttg cgtagcccac aagccgagga
     1201 agacgagaaa gaacctcctc cgctccagaa gcgtatccaa acgcctccgc caaaggctcc
     1261 cccaacagtc gatgttgcgc ctcgccccgt ctcaaaaccc actccttcgt tggaagactc
     1321 tcctcctcca cagcagcgtc ccaccataaa gccagccaat gttctggcgc gtgtcctgca
     1381 gcccgactta agcgctgcgc aagcgccccc taaaagcatt gccgcccaga tcgacgagat
     1441 cctgcaggag atgctggagg attctcccct ggactcgagg ggaatccgct tgatggagtt
     1501 tcccaatcga gggctggtgg tcatggtcgg gctggaccag tacgagggtg tggatgcagt
     1561 tcctgatgag gatatccgca gactaatccg aagcgctgtt catgaatggg agcggcgtaa
     1621 caccccctct tcttagaaat cataacccca gccaaaaagc ggctcgcact ttgtggtcta
     1681 cgcaaaaaga tagcacgtca ttgagagccc caaaggggcg aagcaatctc agctttgcct
     1741 ggagattgcc acgccgccct ccatccggct tgcaatgacg ctacaatcaa attgggagca
     1801 tatgggtggg cgaaatccag ccataggctc ctaattgttt cctcaactgc gcgtttctga
     1861 ttttataggt gggcttggaa ttcttggtct attgactgga cgagcttgct gcgctcggat
     1921 tcgggcaaga aagatgcctt cgccgcagct agcacacgcg ctcgcaggac ttcgaggggg
     1981 aaattcaggg tttcacagaa taaatgatat tcgtggctga ggtcgatctg tgagatgctt
     2041 gggtcatcgg tgttgatggt cacatttaac cctttggcaa gcatctgagc caccgggtgg
     2101 gagttcaagt cggtcaccgc gccggactga tagttgctgg tgatgcatac ttcgaagggg
     2161 gtctggtttt tttgagccag ttctatcgct gtaggtgact cgagcacgcg tattccgtga
     2221 ccgatgcgat ctgcgtccaa tttctcgata gcttcggcta cattgtcgcc atgatcccac
     2281 tcgccagcat gaatcgcaat gcgcagtcca gactctttgg catgctgaaa aatcgaggcg
     2341 aaaggttcag ctgggtatcc aggctcagag cctgccaggt ccagccccac aatacctgca
     2401 tctcgcctct ccactgccag gtcggctacc tgtgcagcca gggtgggacc ctcatgacgg
     2461 ttaacgctgg cgatcaagcg agtggttaac ccgctttctg cctcaccttg gcgggtgcct
     2521 tcaatgaccc aatcgatgac ttgtgccagc gagaaaccct gagatttgct cagcgccacc
     2581 ggagtgaaac gcagctctag atagcgcacg ttgtccgcgg cagcatccgc gatagcttcc
     2641 ctggtgatgc gggtgataat ctcgggtgag cggtagagca ggcgcagggt cgcgaatttg
     2701 gataagaagt tctcgaaggt aagcggctca tccttttgga tctgcaccaa gctgcgcaat
     2761 tgactggcgc ctggcaaatc caggttatgg gagcgggcga tatcacgcac agtctcgaag
     2821 cgtaatgagc cttccaggtg gcggtgcagg tccactttgg gcagcgattg gtagaattcg
     2881 aatggtcgca tccgtggctc ggcgaggttc atgtagttta agtgcttccc atatctttcg
     2941 cagtgaagcc ttcaaagaac tattcggttt taacgccgcc tcctgcggcg tttctttggt
     3001 tttccacccc gcgtttcttt ttctatctgt ggagccgatt ggggtccgtt cccggaaacc
     3061 acggcttcta cctcgggtgc ttcgacacgg ctgacggtgg tttgcacagc actcaggtcg
     3121 cgcggccggt aggtgaacat gcggcctacc actccagagc gcatattacc gagcaaatcc
     3181 tggtacaggc cgaaagcgcg gtttttgtat tgcaccagcg ggtcgcgctg tgcatacgcc
     3241 tccagcccga tggagacgcg cagggcttcc atctgtgtca ggtagtccac ccacagctca
     3301 gtgatgatac ccaatagaag ttgccggtag atctcggtga gcgcttggcg accaagctcg
     3361 ttaacgacac gctccacctt ctcacctggc aagccctgca atggtcgttg gtggacgcta
     3421 agataatcct cctcgctcag cacactgcgc aatccctttt gggttgtagt gtctaggtct
     3481 tgaatggaag atgcagcgat gcgattccat tcattgatgc cccatgcttg ccggatggcg
     3541 gcttgggcaa gctctagatg cttgagcaca tcgtctgcaa tggcctctgg ctcgcggcct
     3601 tccagatagc ttgcggcatg atagacatag ctaaatcgag tggtgcggtg gtagacgcgc
     3661 cggtgagttt tcttatcgaa ggcagcccgc gccccttgcg gcatgagcat aagcaattgg
     3721 agcaaggcat tggatttcaa tttcccgtcc gctcgaccga tggcattttc cagctctcga
     3781 gcgatctggc ctccctcgcc gaggtaacgc tcgcgccgtc cttcaaaggc aagctggatg
     3841 gcagataata cctggtcgct aagatcgtcc caattttcgg caggcagctg gttcatatcg
     3901 atgttgagcg actcttccaa acggcgttct acgctaccga ccagcctagt gaccgcctgg
     3961 gcagacagat atcccatgag ctgctcgtag accaggtcct tggcctcttg cgggtcgtct
     4021 cgcagcaggc ggagttgctc tgatggcaga cggataggca ggcgcactac agtggagagc
     4081 tcgtccgcga tctggcgtag gctgcgctgc tcggtctcgt ctgccaggga taagccttca
     4141 aaaaatatat ctagggtttc atccctctcg gcgatttggc tttccaggcg ctcccgcgcc
     4201 tgctccaata gcatgtcgac ggcgttgact agatgatctt cctcagcctg gatggcttgt
     4261 ctggcgatct ctagcagagc ctggatggca gcttgtggac tcaatccacc cgattggcca
     4321 tccaaaatat gctcgaggat caagcgcaac atgtaagatg gatagaattg tgaaccaaac
     4381 tgaagcggag gctggatttg ctccatccag gccagtagct tccagggacc ttctggatcc
     4441 tgtaaagcct cagggatgcg ctggttgatc tcggtgcgca acatttcggt tacatcttcg
     4501 ctgagatcat ctttaaggaa gatgcggtta cgctg
//
LOCUS       DBNHOH010000076         1277 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_516632, whole genome shotgun sequence.
ACCESSION   DBNHOH010000076 DBNHOH010000000
VERSION     DBNHOH010000076.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_516632"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1242
                     /locus_tag="AC16V7_01340"
     CDS             <1..1242
                     /locus_tag="AC16V7_01340"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HIR6255789.1"
                     /translation="LISANKKSQRAERERNRAIYNLISALNASLNYQRVLETALDLGY
                     STLAKNHESENRMVSSVLLYTKINGNDSELKVGAARRFTTADMRISLPGTTGLIGETI
                     DKAESQLTSEVSNDPELSRIVAVRTCRSAYCIPLFTGLDTYGVLLFAHPDSNYFTSER
                     CEILDIIGNQAIVAMQNARLYHELELEKERMMEIQEEARKKLARDLHDGPTQSVSALA
                     MRVNFTRRVMERDPGAAAEELYKIEELARRATKEIRHMLFTLRPLILESQGLVAALES
                     MADKMRETYNQNVVIEADPEIVSQIDPNRQGVLFYIAEEAVNNARKHAQASNIWVRIK
                     ASADDFALLVIEDDGVGFDLVSVDSSYNNRDSLGMLNMRERTELINGKLKIEASEGKG
                     TLIQVLIPLDEDAAERIRLGQ"
BASE COUNT          345 a          339 c          310 g          283 t
ORIGIN      
        1 ctaatctcag ctaacaaaaa atcccagcgg gccgaacgtg aacgcaatcg ggcaatttac
       61 aacctcatct ccgcactcaa tgcctcactt aattaccagc gtgtcctgga aactgccctt
      121 gatttgggat attctaccct ggccaaaaac cacgaatccg agaatcgaat ggtcagcagt
      181 gtattacttt atacaaagat aaatggaaat gattcagagc tgaaagtggg cgcagccagg
      241 cgcttcacca cggcagatat gcgcatctcc ctacctggga cgaccggctt gattggcgag
      301 acaattgaca aggcagagtc gcaattgacc agcgaggtca gcaatgatcc ggaattgagc
      361 cgcatcgtgg cagttcgtac ctgccggtct gcctactgca ttcctctctt taccgggctc
      421 gatacatatg gtgtgctcct tttcgcccat ccagatagca actacttcac cagcgagcgc
      481 tgtgagatac tggatatcat cggcaaccaa gctatagtag ctatgcagaa tgcccgcttg
      541 tatcatgaac tagaacttga gaaagagcgt atgatggaaa tccaggaaga ggcccgcaag
      601 aagctcgccc gcgatctgca tgatggccca acgcaatccg tatccgccct ggcgatgcgc
      661 gtaaacttca cccgccgcgt gatggagcgc gatccaggcg cagcggccga agagctttat
      721 aagatagaag aactggccag gcgtgctacc aaagaaattc ggcacatgct tttcacgcta
      781 cgcccgctta tcctcgaatc ccaggggttg gtagcagccc tggaatctat ggcggataag
      841 atgcgcgaaa cctacaacca aaatgtggta atcgaagctg acccagagat tgtctctcaa
      901 attgacccga acaggcaggg cgtgctcttt tatattgcgg aagaagcggt caataacgcc
      961 cgcaaacacg cccaagcctc gaacatctgg gttcgaataa aagcatccgc agatgatttc
     1021 gcgctgcttg ttattgaaga tgatggagtg ggtttcgatc tcgtatccgt tgattcatcc
     1081 tacaataacc gcgacagtct cggtatgttg aacatgcgcg agcgcaccga attaatcaac
     1141 ggcaaactta agattgaagc gtctgaagga aaaggcactc taatccaggt gctcatacct
     1201 ctcgatgaag atgccgccga gcgcatccgt ctcggtcagt aatacttttt atatgcccat
     1261 cgctgatagc atctact
//
LOCUS       DBNHOH010000077         1418 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1541514, whole genome shotgun sequence.
ACCESSION   DBNHOH010000077 DBNHOH010000000
VERSION     DBNHOH010000077.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1541514"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1073)
                     /locus_tag="AC16V7_01345"
     CDS             complement(30..1073)
                     /locus_tag="AC16V7_01345"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6255790.1"
                     /translation="MAGFWCTIDAIFMASLTKLLASSSPSGRLRPVDIRRDLNPVADL
                     MENSFSETLDPDGRRYLQQMRSAANNPGFLRWAGLAAEGARVPFSGYVWEEDGQVVGN
                     LTLIPYYTLGLRYMLVANVAVHSNYRRKGIATSLTLKAIEHARGRGAQAIWLHVREEN
                     EGATRMYRSLGFVERARRTTWVTDEVQPPGSVGPVINQGVRITPGISVHPRRASDWRL
                     QHDWLEKLYPAQLSWHLSLKFNALRPDLWGLLIRVLNDIHVRQWSARQNDRLAGVLTW
                     QLMPAYADSLWLATAPEFEEEAAGALLQYARSHLPSRRPLSLDYPAERASQAIQAAGF
                     HLHQTLIWMSMDL"
     gene            1178..>1418
                     /locus_tag="AC16V7_01350"
     CDS             1178..>1418
                     /locus_tag="AC16V7_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255791.1"
                     /translation="MSLDFQQVRQQVKKLGEDAPSREDRLKALRQQARDLLQVNAGEL
                     ESLRQKVQAVVRNHDPSLRCAMPTFERLNASYPLPV"
BASE COUNT          355 a          403 c          375 g          285 t
ORIGIN      
        1 gaccaggatg atcaggataa cattgagggt cataaatcca tagacatcca aatcagtgtt
       61 tggtgcaagt gaaaaccagc agcctggatc gcctggctag cccgctcggc tggataatct
      121 aacgacagcg gtcgacgcga aggtagatga gagcgggcgt attgaagcag tgcaccagct
      181 gcttcttctt cgaattctgg cgctgtcgcc agccaaaggc tatccgcata cgccggcata
      241 agctgccaag tcaaaactcc cgctaaccga tcattttgac gcgcagacca ctggcgcaca
      301 tggatgtcat tcaagacgcg gatcaatagc ccccataaat cagggcgcag ggcgttgaat
      361 ttgagcgaca ggtgccagct cagctgagca ggatataact tctccagcca atcgtgttga
      421 aggcgccaat cgcttgcgcg tcgtggatgt acactgatac ccggagtgat gcgaaccccc
      481 tggttaatca caggccccac tgagccaggc ggctgcactt catctgttac ccaggttgtg
      541 cggcgtgccc tctcgacaaa ccccaaggag cgatacatgc gggtcgcgcc ctcgttctct
      601 tcacgcacat gcaaccagat agcctgtgcg ccgcgccccc gggcgtgctc gatcgctttt
      661 aaggtcaggc tggtagcgat ccccttgcga cgataattag aatggacggc gacattggca
      721 accaacatat acctgaggcc aagcgtgtaa taagggatca atgtgagatt gccaaccacc
      781 tgtccatctt cttcccagac atatcccgag aaaggcaccc tggccccctc tgcggccaaa
      841 cccgcccagc gaaggaatcc cgggttgttg gcagccgaac gcatttgctg caagtaacgc
      901 cgcccatccg ggtccagcgt ctcggaaaag gagttctcca tcaggtcagc gacagggttc
      961 aaatcccggc gaatatctac ggggcgcagt ctcccagatg gagatgaaga ggcgagcaac
     1021 ttggttagag aggccataaa tatggcatcg attgtacacc agaatccagc cattgcccaa
     1081 aattttctcc ttggacttta ccatgatcat ggtaaagcca tttagtcaaa aactggtaca
     1141 attgatcaaa caaatgttct gagatgaagg gatcgccatg tcgctagatt ttcagcaggt
     1201 gcgccaacag gtcaagaaat tgggtgagga tgcgcccagc cgggaagacc gcctcaaggc
     1261 gctccgccag caagccagag acttgcttca ggtcaatgca ggcgaactag agagcttgcg
     1321 ccagaaagtg caggcagtcg tgcgcaatca cgatccgtct ctgcgctgtg ccatgcccac
     1381 ctttgagcgc ctgaatgcca gctatcccct accagtaa
//
LOCUS       DBNHOH010000078         3734 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1477711, whole genome shotgun sequence.
ACCESSION   DBNHOH010000078 DBNHOH010000000
VERSION     DBNHOH010000078.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1477711"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="AC16V7_01355"
     CDS             complement(<1..989)
                     /locus_tag="AC16V7_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6255792.1"
                     /translation="MADDQSTSSTKPEALVQVRELKKYFPVKRGLIFEREVGAVKAVD
                     GISFDIRKGETLGLVGESGCGKTTAGRVILGLYPITDGSVTISGIQVDQASKQELQTL
                     RRKAQMIFQDPYASLNPRWTVNAIIGEPLRVHKLLPNNKARMDRVNELMLLVGLSPRL
                     INRFPHEFSGGQRQRIGVARALASSPEFIVCDEPISALDVSIQAQVVNLLEDLQDEFN
                     LTYLFIAHDLSMVRHICDRVAVMYLGVIVELTGKDELYENPLHPYTQALLSAVPIPDP
                     KKGRARQRTILPGDVPSPINPPSGCRFHPRCPIAKGHCSQIVPEWREISEGHWV"
     gene            complement(1009..1998)
                     /locus_tag="AC16V7_01360"
     CDS             complement(1009..1998)
                     /locus_tag="AC16V7_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019955731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6255793.1"
                     /translation="MADRLLEVNDLRTRFHIAEGTVHAVNGISFYLEHGKMLGVVGES
                     GCGKSVTMLSLLRLLPMPPAEITSGQVLYLGRDLLQISEHEMETLRGKEIAMVFQDPM
                     TSLNPVLTVGRQITETLRAHMGMEKQMARERAIELLDQVGIPDSARRLDDYPHQFSGG
                     QRQRAMIAMALACVPSILIADEPTTALDVTIQAQIVELVQRLRANLDMAVIWITHDLG
                     VVAEMAERVLVMYAGFIVEEALVDDLYEDPRHPYTIALLAALPRIDRRRDKRLRSIPG
                     APPNLLVEPTGCPFAARCELAFDRCRTEMPPLVQINPNHQAACWVDVTTGKPR"
     gene            complement(2012..2974)
                     /locus_tag="AC16V7_01365"
     CDS             complement(2012..2974)
                     /locus_tag="AC16V7_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6255794.1"
                     /translation="MTETAVSQPKPGIASLTPDELETPPLTLGQLTWRRFRRHRMAIF
                     GAVTLILMILYCFGGMLVFSEADANFVETGNRLSAPSREHPFGTDTIGRDILVRTIYG
                     GQISILIGLTAMLVELLIGITFGALAGYFGGILDSILMRITEAVLVIPQIFLLLVMAK
                     YFAQDIPNIQFLGRTLSGSVLVIIFVIGISSWPYLARIVRAQFLSVKENDFILAARAT
                     GTSTRDIIFRHILPNSIAPIIVSATLSVAAAITLEAYISFLGLGVRPPTATWGNMLEG
                     AYNYVESAWWLWFFPGVLIALTVLSINFLGDGLRDALDPHSRAI"
     gene            complement(2984..>3734)
                     /locus_tag="AC16V7_01370"
     CDS             complement(2984..>3734)
                     /locus_tag="AC16V7_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6255795.1"
                     /translation="LRGDWGISLVNRQPVTEVISQRLSNTLLLMLTAEALIIVFALII
                     GIYSALNQYSALDNTITATTFVFYSMPVFFFALLAMYIFAVNFKKWGLPYLPTVGMFE
                     PEAGQTPSQVLWHMVLPVATLSFGFIAGYSRYIRSTMLEALGQDYVRTAKAKGLPRPE
                     IIYIHALKNASLPIVTIIALDLPFLLGGAIVTERIFGWPGMGRLFLDHVARSDTPVVM
                     GILMMIAAAVIVFQILADITYAWLDPRIRYK"
BASE COUNT          796 a         1043 c         1160 g          735 t
ORIGIN      
        1 acccagtgac cctcggagat ctctcgccat tcggggacta tctgcgagca gtgccccttg
       61 gcgattgggc agcgcgggtg aaagcggcag ccggagggcg gattgatcgg gctaggcaca
      121 tctccgggga ggatggttcg ctggcgggcg cgacccttct tcgggtcggg aatgggtact
      181 gccgagagca gcgcctgggt ataaggatgc aatgggtttt cgtatagctc gtctttcccg
      241 gtcagctcga caattacccc caggtacatc accgccaccc ggtcgcagat atgccgcacc
      301 atgcttaaat cgtgggcgat gaacaggtat gtcaggttaa actcgtcctg caggtcttcc
      361 agcaagttga ccacctgcgc ctgaatggag acatccaggg cggagatcgg ctcatcgcaa
      421 acgatgaatt ccgggctgga tgccagggcg cgcgccacac cgatgcgctg gcgttgcccg
      481 ccggagaatt catgcgggaa gcggttgatt aacctcgggc tcaaccccac cagcagcatc
      541 agctcgttga cgcggtccat gcgcgccttg ttgttaggca gcagcttgtg cacccgcaag
      601 ggctcgccaa tgatggcgtt gaccgtccag cgcgggttga gcgaggcgta gggatcttgg
      661 aagatcatct gcgctttgcg acgtagtgtt tgcagctctt gcttgctggc ctgatccacc
      721 tggatcccgg agattgtgac agaaccatcg gtgatggggt acaggcctaa aatgacgcgc
      781 ccggcggtgg tcttgccgca accgctctcg cccaccaggc ccagcgtttc ccccttacgg
      841 atatcaaatg atataccatc tacggctttg accgcgccga cttctctctc gaaaataaga
      901 ccacgcttga ctggaaaata cttcttcagt tcacgcacct gcaccagtgc ttcgggtttt
      961 gtagaggaag tggattggtc gtcagccaag ggatatctcc aaataggatt agcgcggttt
     1021 accggtggtc acgtctaccc agcatgcggc ctgatggttt gggttgatct gtactaaggg
     1081 cggcatttcg gtccggcagc ggtcgaaggc caattcgcag cgcgccgcaa acgggcagcc
     1141 cgttggctct accagcaagt tgggcggcgc cccgggtatg gagcgcaggc gcttgtcccg
     1201 gcggcggtcg atgcgcggca gtgctgccag cagcgcaata gtgtacgggt ggcgcgggtc
     1261 ttcgtacagg tcgtccacca gcgcttcttc gacgatgaag cccgcgtaca tcaccagcac
     1321 ccgctcagcc atctcagcca ccacacccag gtcgtgggtg atccagatga cagccatatc
     1381 caggttggcc cgcaagcgct gcaccagctc gacaatctgg gcctggatgg tcacatccaa
     1441 ggcggtggtg ggttcatcgg cgatcaagat gctcggcacg caagccaggg ccatggcgat
     1501 catggcacgc tggcgctgcc ctccagagaa ctggtgcggg taatcatcta aacgccgggc
     1561 agagtcgggg atgccgacct ggtcaagcag ttcgatagcc cgttcgcggg ccatttgctt
     1621 ctccatcccc atgtgtgccc tgagcgtttc ggtaatctgg cgaccgacgg tcaacaccgg
     1681 gttgagtgag gtcatcgggt cctgaaagac catggcgatc tctttgccgc gcagagtctc
     1741 catctcgtgt tcgctgatct gaagcagatc acgccccaga tatagcactt gcccactggt
     1801 aatctcagcc ggtggcatgg gtagcaatcg cagcagggac agcatggtga ctgattttcc
     1861 gcagccgctc tcgcctacca cgccaagcat tttgccatgc tccaggtaga aagaaatgcc
     1921 attaacggca tggacggtgc cttcggcgat gtgaaatcga gtgcgcaggt cgttgacttc
     1981 gaggaggcgg tccgccatct tattctcccg gctatattgc acggctatgg gggtcgaggg
     2041 cgtcgcgcag cccgtcgccc aggaagttga tgctcagcac ggtcaaggca atcagaacgc
     2101 cggggaagaa ccataaccac caggcggatt caacgtagtt gtaggcgcct tccagcatat
     2161 tgccccaggt ggcggtgggg gggcgcacgc ccaaccccag gaagctgatg taagcctcca
     2221 gggtgatggc cgccgccaca ctcagggtag ccgacacgat gatgggggca atgctgttag
     2281 gcaggatgtg gcggaagatg atatccctgg tagacgtacc ggttgcccgc gctgccagga
     2341 tgaagtcgtt ctctttgaca gagagaaatt gggcgcggac gatgcgcgcc aggtagggcc
     2401 agctggagat gccgatgacg aaaatgatga ccaggacgct gccgcttaag gttcgcccga
     2461 ggaactggat gttgggaatg tcttgggcga aatatttggc catcaccagc agcaagaaga
     2521 tctgagggat aaccagcacc gcctccgtga tgcgcatcag gatgctatcc agaatgcctc
     2581 cgaagtagcc cgccagtgcc ccgaaggtga ttccgatcaa caactcgacc agcatggcgg
     2641 tcaggccgat caggatggag atctgcccgc cataaatcgt gcggaccagg atgtccctgc
     2701 caatggtatc ggtgccgaag gggtgctcgc gagagggcgc tgaaagacgg ttacctgttt
     2761 cgacgaagtt ggcatcggct tcgctgaaga ccagcatgcc accaaaacag tacaagatca
     2821 ttaagatcaa cgtcaccgcg ccgaaaattg ccatcctgtg ccggcggaag cggcgccagg
     2881 tgagctggcc aagggtcagg ggaggggttt ccagctcgtc gggcgtcagg ctggctattc
     2941 caggcttggg ttgtgagact gctgtttcgg tcatgatagc tcgctattta taacgtatcc
     3001 tggggtccag ccaggcgtag gtgatgtcgg ccaagatctg gaagacgatc accgccgcgg
     3061 cgatcatcat caagatgccc atcaccaccg gggtatccga gcgtgcgaca tggtccagga
     3121 acagacgtcc catacctggc cagccgaaga tgcgctcggt gacgatcgcc ccgcccagca
     3181 agaaaggcag gtctagggcg atgatggtga caatgggcag ggaggcgttt ttcaaggcgt
     3241 gaatatagat gatctctggt cggggtagcc ccttggcctt agcggtgcgc acatagtctt
     3301 gccccagggc ttccagcatg gtgctgcgga tgtagcggct gtaaccggcg atgaaaccga
     3361 aagatagagt cgccaccggc aggaccatgt gccacagcac ttgggagggc gtctgcccgg
     3421 cttccggctc gaacatgccc acagtcggta agtagggcaa cccccatttc ttgaagttga
     3481 ccgcaaagat gtacatcgcc agcagggcga agaaaaacac cggcatagag taaaaaacga
     3541 aggtcgtggc cgtgatggtg ttatccagcg ctgaatactg gttcaacgct gaatagatac
     3601 cgatgatcaa ggcgaaaaca atgatcaatg cttcggcggt cagcatcaag agcaaggtat
     3661 tggacaagcg ctggctgatc acttcggtga ccggttgccg gttgaccagc gagatgcccc
     3721 aatcaccgcg cagc
//
LOCUS       DBNHOH010000079         3525 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_260099, whole genome shotgun sequence.
ACCESSION   DBNHOH010000079 DBNHOH010000000
VERSION     DBNHOH010000079.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_260099"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="AC16V7_01375"
     CDS             complement(<1..237)
                     /locus_tag="AC16V7_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HIR6255796.1"
                     /translation="MQVKIVLLPGDGIGPEVTGAAMQVFEALAGLYGHAFAFTEHLIG
                     GCSIDAHGTALTDEALADCQAADAILLGAVGGPKW"
     gene            complement(248..1801)
                     /gene="cimA"
                     /locus_tag="AC16V7_01380"
     CDS             complement(248..1801)
                     /gene="cimA"
                     /locus_tag="AC16V7_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HIR6255797.1"
                     /translation="MIQIYDTTLRDGTQRTDISLSVNDKLNIARRLDDLGIAYIEGGW
                     PGSNPKDAEFFQRAADIPWRTAQIAAFGSTCGANKHPEDDENINALLSAGTSTCTVVG
                     KTSTLHVLEVLRTSRENNLRMIEESIAYLRSQGKRVLYDAEHLFDGYKEDQAYALETL
                     KAALHGGAETIILCDTNGGTMPWEITEIVGAIKAELNHPLGIHTHNDGECAVANTLAA
                     VLEGCIQVQGTINGYGERCGNANLCSIIPDLELKMGYSCLPDGNLTKLFDLSHYVAEV
                     ANLAPDEHLAYVGRSAFAHKGGIHVAAMRRNPNSYQHIDPEHVGNEMAVVVSELSGRG
                     NLLSKAEEYGLDTSNVGTVGEVLSEVKTLEAKGFSFETAEASVLLMLQRQQNGYKPPF
                     DLIDFSVTVEHRQGRGIFAEATVKVRVNGDILHTAAEGNGPVNALDAALRKALVTHYP
                     EVNQFKLADYKVRILDGNNGTAATTRVLIDTQNGTKSWSTVGASTNIIEASWRALADS
                     VEYGLMVMK"
     gene            complement(1835..2425)
                     /gene="leuD"
                     /locus_tag="AC16V7_01385"
     CDS             complement(1835..2425)
                     /gene="leuD"
                     /locus_tag="AC16V7_01385"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIR6255798.1"
                     /translation="MQPFKKLTSRVTVMLVNDIDTDQIIPARFLKTVEKAGLAANLFA
                     DWRYLPDGSPNPEFVLNQPGGQGASVLLAGQNFGCGSSREHAPWALTGYGFRAVISTS
                     IADIFHNNALKNGLLPVVVSPEDHQRMVDLFSEDPNAELTIDLAEQAVYLPDGHTVSF
                     PIDGFAKSCLLNGVDELGYLMSIEDKITAYETQMTN"
     gene            complement(2526..>3525)
                     /locus_tag="AC16V7_01390"
     CDS             complement(2526..>3525)
                     /locus_tag="AC16V7_01390"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR6255799.1"
                     /translation="FGALAFGIGTSEVEHVLATQCLLQRRPKTCEVRIDGTLPPGVTS
                     KDIILALISKIGVGGGTGHVFEYSGSAIRALTMDQRMTLCNMSIEAGARAGMIAPDDT
                     TFEYLSGREFAPRGQAWESSLRWWRELSTDDGATFDRTISLDASTLEPMITFGTNPGM
                     GIPVNGHVPTIDEVKDATQKVALEKALNYMGLSPGQTLVGHPVDVVFIGSCTNGRIAD
                     LRQAAGLLKGRKVASGMRVLVVPGSQQVKRQAEVEGLDQVFLAAGAEWREPGCSMCIA
                     MNGDQLQPGQYAVSTSNRNFEGRQGKGGRTFLASPLTAAATALAGKVTDVRTILPN"
BASE COUNT          770 a         1033 c          914 g          808 t
ORIGIN      
        1 ccacttcggc ccgcccaccg cgcccagcaa gatggcgtcg gcagcctggc aatcggctag
       61 cgcctcgtcg gtgagggctg tgccatgcgc gtcgattgag cagccgccaa tcaagtgctc
      121 ggtgaaagca aaggcatgac cgtacagacc agccagagcc tcgaagactt gcatggctgc
      181 accggtcact tccggaccta tgccatcgcc aggtaataaa acgattttta cctgcataaa
      241 caccaaccta cttcatcacc atcaagccat attcaaccga gtcagctagc gctcgccagc
      301 tggcctcaat aatattggtg ctagctccaa cagtgctcca actcttggtg ccgttttgtg
      361 tatcgatcag cacgcgtgta gtcgccgcag tgccgttatt cccgtccagg atgcgcactt
      421 tatagtcagc cagcttgaat tggttgacct cgggatagtg cgtgaccagc gccttgcgca
      481 gggcggcatc cagcgcattg actgggccgt tcccttccgc agccgtatgc aggatatctc
      541 catttacgcg caccttgaca gtggcttcgg cgaaaatccc tcgtccctgg cggtgttcaa
      601 ccgtcaccga gaaatcgatg agatcgaagg gcggtttata gccattctgc tggcgttgca
      661 gcatcaggag gacggatgct tcagcggttt caaaagaaaa tcctttcgct tccagggttt
      721 tcacctcgct caagacctcg ccaaccgtgc cgacattcga cgtatccaac ccgtactctt
      781 cggccttgct gagcaagttt ccgcgcccgg aaagctcgga gaccaccacc gccatctcgt
      841 taccaacgtg ctctgggtca atatgctggt atgaattggg gtttcgccgc atagctgcca
      901 catggatacc gcccttgtga gcgaaagctg agcgacccac atatgccagg tgctcatcag
      961 gtgccaggtt ggcgacctcg gccacatagt gagagaggtc aaacaacttg gtcagattcc
     1021 catctggcag gcacgaatag cccattttca actcgaggtc gggtatgatc gaacaaagat
     1081 tggcatttcc gcaacgctcc ccgtaaccgt tgatggtgcc ctgcacctga atacaacctt
     1141 cgagcaccgc ggcaagcgtg ttagcgacag cacattcacc atcattgtga gtatggatgc
     1201 ccaaaggatg attaagctcg gctttaatgg cgccgacgat ttcggttatc tcccagggca
     1261 ttgtgccccc gttggtgtcg cacaagatga tggtctctgc cccaccgtgc agggctgctt
     1321 tcagggtttc gagagcatac gcctgatcct ctttgtagcc atcgaagagg tgctctgcgt
     1381 catacaacac acgctttccc tgcgagcgca ggtaggcgat gctctcctcg atcatgcgca
     1441 agttgttctc gcggctggtg cgcagcacct ccaagacgtg cagggtcgag gtttttccca
     1501 ccaccgtgca ggtgcttgtg ccggctgata acaaggcgtt gatattctca tcatcttctg
     1561 gatgcttgtt agcgccgcaa gttgaaccga aagccgcgat ttgagctgtt cgccagggga
     1621 tatcggcagc gcgctggaag aactcggcgt ccttggggtt tgagcccggc cacccacctt
     1681 cgatataggc aatgcccaaa tcgtccaacc ggcgggcaat gttcaactta tcgttgacag
     1741 agagtgagat atcggtgcgc tgcgtcccat cacgcagggt cgtatcatat atctggatca
     1801 ttttcatgct tgaatttgct tccctttcgc tggcttaatt ggtcatctgg gtttcgtaag
     1861 cggtaatctt atcctcaatg ctcatgagat aacccagctc atccacgcca ttgagaaggc
     1921 aagacttggc aaaaccatct attggaaagc tcaccgtatg cccatctggc agatataccg
     1981 cctgctctgc cagatcgatg gtcaactccg cattgggatc ttcactaaac aagtcaacca
     2041 tgcgctggtg atcttcaggg ctaaccacca cgggcagcag gccatttttc agggcattgt
     2101 tgtgaaaaat atctgcaatc gatgtggaga taaccgcccg gaaaccatag cccgtcaaag
     2161 cccagggagc gtgttccctg gacgagccac agccaaaatt ttgacccgcc agcaacacgc
     2221 tggcgccctg accacccggc tggttgagga caaactccgg attcggtgac ccgtcaggga
     2281 gatagcgcca atcggcaaac aagttagccg ccaggcctgc tttctcgact gttttaagaa
     2341 agcgggccgg gataatctgg tcggtgtcaa tatcattgac tagcattacc gtgacacgtg
     2401 aggtgagctt cttgaatggt tgcatattta tcgtcactct tgttgtagct tgcgaaaata
     2461 accctgctat gtgtttctct cgggtgcggg tttaaaaacc cggctgtgcc agcaaacttc
     2521 cataattaat tggggaggat ggtcctcaca tcggttacct tgcctgccag ggctgtcgca
     2581 gccgcggtga gcgggctggc caagaaagtc cgtccaccct ttccctgccg cccttcgaag
     2641 ttgcggttgc tggtgctgac ggcgtattga ccgggctgta actggtcgcc attcatggca
     2701 atgcacatgc tgcagccggg ctcccgccat tcggcacccg cggcgaggaa gacttgatct
     2761 aagccttcta cctccgcctg gcgcttgact tgctgggagc caggtacaac cagcacacgc
     2821 atcccagacg ccaccttgcg cccctttaat agtccagcag cctgccgcag gtctgctatg
     2881 cgaccgttcg tgcaacttcc aataaagacc acatctacag gatgcccgac aagtgtttgc
     2941 cctggactca accccatgta gttcagcgct ttttctaagg ctaccttctg ggtggcatct
     3001 ttgacctcat ctatcgtggg gacgtgccca ttcaccggta tacccatgcc tggattggta
     3061 ccaaaggtga tcataggttc cagggtagag gcatccaggc tgatcgtcct atcaaaagtg
     3121 gcgccatcat cggtggatag ttcccgccac cagcgcaagc tgctctccca tgcctgcccg
     3181 cgtggggcaa actcccgccc agaaagatac tcaaaggtag tatcatccgg ggcgatcata
     3241 ccggcccgcg ctccagcttc tatcgacatg ttgcagagag tcatgcgctg atccatggtc
     3301 aaagcgcgaa tagctgaacc gctatattca aaaacatgac cagtcccccc gcccacaccg
     3361 atcttgctga tcagtgccag gatgatatcc ttggaggtca ccccaggggg tagcgttcca
     3421 tcaatgcgca cctcacaggt cttagggcgg cgctggagca agcattgggt cgccagaacg
     3481 tgctcaactt cgctggtgcc aattccaaaa gccaatgcac caaat
//
LOCUS       DBNHOH010000080         1588 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1157546, whole genome shotgun sequence.
ACCESSION   DBNHOH010000080 DBNHOH010000000
VERSION     DBNHOH010000080.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1157546"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="AC16V7_01395"
     CDS             <1..1081
                     /locus_tag="AC16V7_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006727758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HIR6255800.1"
                     /translation="LGIHPRELGAAPFAPAVSSALDLKARELGLAFHPAARLHTLPLI
                     AGHVGADNVAVLLAEAPNEQDDVMLIVDVGTNAEIVLGNRQRLLAASSPTGPAFEGAQ
                     ITHGQRAAPGAIERVRIDPETLEPRFKVIGYESWIEPGAELPHEAKPTGICGSGIIEA
                     VSEMLLAGIIRPDGLFDRSMTERSPRVNYQGRTGEYILADASLTATGLAIVITQNDIR
                     AIQLAKAALYAGTKLLMNHWGAERVDKVVLAGAFGSFISPYHAMLLGLIPDCDLENVY
                     AVGNAAGDGARFALLNRNLRSKAAQLALWVEHVSTPLESSFQDEFVAALALPHARDPF
                     PTLQAFLPQVEGDPKPGRRNRVRDR"
     gene            1230..>1588
                     /locus_tag="AC16V7_01400"
     CDS             1230..>1588
                     /locus_tag="AC16V7_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009604472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HIR6255801.1"
                     /translation="MSDEIDISQLETPEILELIQDDLYDGLADEVVKSVKELLSRSMT
                     PYDVLTKGLVAGMDVVGVDFRDGILFVPEVLMAAKAMKAGMEILRPLLAETGAPQVGT
                     MVIGTVKGDIHDIGKNLV"
BASE COUNT          329 a          432 c          494 g          333 t
ORIGIN      
        1 cctgggtatc catccccgtg aactgggggc ggcgccgttt gcccctgcgg tgagctctgc
       61 cctagaccta aaagcgcgtg agctgggcct ggcattccat ccggcagctc gcttacacac
      121 cctgccgctg atcgctgggc atgttggggc agataatgtg gcggtgttgt tagccgaggc
      181 gccaaacgaa caggatgatg tcatgctgat cgtggatgtg ggcacgaatg ccgagatcgt
      241 attaggcaac cgccagcgcc ttctggcagc ctcaagcccc accggaccag ccttcgaagg
      301 cgcgcagatc acccatggac agcgcgccgc gccgggagct atcgaacggg tgcgcatcga
      361 cccagagaca ctggaaccgc gcttcaaggt tatcggttac gaatcctgga ttgaaccggg
      421 cgcagagcta ccccacgagg ccaagcccac gggtatctgc ggcagcggga tcattgaggc
      481 ggtttcggag atgcttctgg ctgggattat caggccggat ggtctcttcg atcgctccat
      541 gacggaacgc tcgccgcgcg tgaactacca gggtaggacg ggtgagtata tcctggccga
      601 tgccagcctg acggctacgg gtctagcgat tgtcatcacg cagaatgaca tccgcgccat
      661 ccagctggcc aaagcagccc tgtatgccgg aacgaagttg ttgatgaacc actggggtgc
      721 ggagcgggtg gataaggtcg tgctggcggg cgcttttggt agttttatca gcccgtacca
      781 tgccatgctg ctgggcttga tccccgattg cgacctggaa aatgtttacg cggtgggtaa
      841 cgccgccggc gatggggcgc gcttcgcgct gctcaatcgc aacttgcgct cgaaagcggc
      901 ccagctggct ctgtgggtgg agcacgtttc gaccccattg gaatcgtctt ttcaagatga
      961 gtttgtggct gccctggcgt tgccacatgc ccgcgatcct ttccccactt tgcaagcatt
     1021 tttaccgcaa gtggaaggag atccaaagcc cggtcggcgc aatcgtgtgc gggaccgata
     1081 atagttaaat ttgttgaagc taccctgtgg tcaacactgg aatgggtagc gaaaaaaagc
     1141 aagaatgcct cgtgggcgcc tttgtgcccg caacacacaa acgttgattt gtgtataata
     1201 cctaaactgc tcgaaggagt acttaataca tgagcgacga aatcgatatt tctcaactgg
     1261 agaccccaga gatacttgag ttgatccagg atgacctgta cgacggcctg gcagacgagg
     1321 tggtcaagag cgtcaaggag ctgctctcgc gcagcatgac cccttacgac gtgcttacca
     1381 agggattggt agccggtatg gatgtcgtgg gcgtggactt tcgcgatggc atcctgtttg
     1441 tgccagaagt gctgatggct gccaaggcga tgaaggccgg catggagatt ctgcgcccgc
     1501 tgctggccga gaccggcgcg cctcaggttg gcaccatggt gatcggcacc gtcaagggcg
     1561 atatccacga tattggaaag aacctggt
//
LOCUS       DBNHOH010000081         3768 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1221681, whole genome shotgun sequence.
ACCESSION   DBNHOH010000081 DBNHOH010000000
VERSION     DBNHOH010000081.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1221681"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="AC16V7_01405"
     CDS             <1..776
                     /locus_tag="AC16V7_01405"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR6255802.1"
                     /translation="FVVADFAGAVGIETVKYVSNLYDVFELEPLDEALIPKDPVKPIF
                     AFNIGLGAFLGLALGIGVVFLVQYIQAPHDIEMEYFNIIDRDTGAFNKSYFLLRLWQE
                     MSRAKRNKYPLSLGLIKIEYINSGNDLPSSSNQAEALRRAKILTEKILREEDILARFD
                     TDILALLLPDMSGEKARSVMENFMSEISAIAYDVGGQDHKFYLKSSVGVASYQNYLLK
                     QDQFLQQAIQALESANIGSNGEVILFSNNHFHKAFKSSN"
     gene            962..2530
                     /locus_tag="AC16V7_01410"
     CDS             962..2530
                     /locus_tag="AC16V7_01410"
                     /inference="COORDINATES: protein motif:HMM:NF024832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HIR6255803.1"
                     /translation="MIDRAINAGMSSSSRERSSERNIVAIAKDGGITFAGKMFLSAVR
                     LVTAVIVARLLGADQYGMYNLALSTANIAIGLAVFGLDSALIRYIAIQASRKDEKGVW
                     GTLQVGVGVATLLSVITGTFIFGLAYYLAETVFHEPRLVPLLQFTGVIVPLLTMSEVL
                     AGALRGFKRMDYPVIAQFVFQPVVRLIMIVVLAFTGFNAVHAILTFGLADLAASIMLI
                     YFLNKEFSLRRSVRAARRDFRTIFEFSIPVWLSEMLVKFHGNIQTLILGSLNTITGVG
                     IFSVASQISMVSSQFSSSINTSAKPVIAELHDRADMREMGQIYQTANKWAVMVQIPIF
                     ITMVLFPAPILSLFGESFTDGALALSIIALADLLKVGTGMGGIIIDMTGYTKLKLVNS
                     IFRLVANLGLDILLIPRWGLLGAAVAALIGEGFINLLRLFQVFFLFRLIPYNRTFIKP
                     VIAGVITLASAFLIKTWLILDGGLFFVIANTGLLLAVYLGITLLMGFSPEDQAVIGFI
                     RQRANKLFSKNSGK"
     gene            2566..3588
                     /locus_tag="AC16V7_01415"
     CDS             2566..3588
                     /locus_tag="AC16V7_01415"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HIR6255804.1"
                     /translation="MGKGPIFIGGTDRSGKTTLRAFLVSHPNISIPANGSNMWSYFYG
                     QFGDLSQAENFERCLDALMHYKHALYLKPDPDRIRHEFWQGTPTYARLFALFHEHLAE
                     REGKPRWGDQTGLIERYADQIFEAYPNAKMIQMIRDPRDRYQASLTLWPKGKGRAGGA
                     TARWLYSTSLAIRNLRRYPNRYMIVRYETLVRDTEQTLREVCTFLDEDFIPSMITMDG
                     DLDRRSRLTQGLNIKPDTNPLTPDFIGLYRQAVPQKEIAFIQGIAGKQMKAIGYSLDA
                     ISFSLSDRIRYILLDWPLNMLRMLAWLSLEIAHHNFPAQFGRKLSSNMITPRKHQNNK
                     VNARAT"
     gene            3585..>3768
                     /locus_tag="AC16V7_01420"
     CDS             3585..>3768
                     /locus_tag="AC16V7_01420"
                     /inference="COORDINATES: protein motif:HMM:NF024860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HIR6255805.1"
                     /translation="MMKTMGVGELLGDRGPVFIGGIQRSGKTYLRFMLSSHSPLVFTR
                     RTNMWPNIYNRFGDISQ"
BASE COUNT          992 a          854 c          887 g         1035 t
ORIGIN      
        1 catttgtggt agctgatttt gcgggcgctg ttggaattga gacggtcaag tatgtcagca
       61 atctatacga tgtctttgaa ctcgaacccc tggacgaggc cctgatacct aaagatcctg
      121 taaaaccgat ttttgccttt aatattggcc taggcgcatt ccttggattg gctttaggaa
      181 ttggggttgt tttccttgtt caatacattc aagcgcccca tgatattgaa atggaatact
      241 tcaatattat cgatcgggat accggtgcat tcaacaaatc gtacttctta ttgaggttat
      301 ggcaggagat gagccgcgcc aaacgcaata aatatccact atccttgggt ctgatcaaaa
      361 ttgagtatat aaattcagga aatgatcttc cctcttcttc caatcaagct gaggcgcttc
      421 gccgggccaa gattctcaca gaaaaaatat taagagaaga ggacattttg gctcgcttcg
      481 acactgatat tttggcattg ttgctacctg atatgtctgg agaaaaagca agatcagtga
      541 tggaaaactt catgtcagaa atcagtgcaa ttgcctatga tgtggggggc caagatcaca
      601 aattctactt gaaaagctcg gtgggggtag cttcatacca aaactatctg ctaaaacaag
      661 atcagttcct tcaacaagct atacaagccc ttgagtcagc taacatagga tcgaatggag
      721 aagtaattct tttctccaat aatcatttcc ataaagcatt caaaagcagt aactgatcat
      781 ccaaagatct aacaagcttg caggaaaacc aaagagagcg cagccaacac cccaccaacg
      841 ataattaagg gttgggagtc atactcttta tataactaag agtacaccag atttttgagt
      901 gatgataatt aaaattgatc gatgattccg ctatgccggt acctcccata tgagtgaaaa
      961 agtgattgac agggccatca acgcaggcat gtccagttct tcgcgagaac gaagctctga
     1021 acgaaacatt gttgccattg ccaaagacgg agggattaca ttcgcaggca aaatgttcct
     1081 ttccgcagtt cggctggtga ctgcggttat cgtcgcacgc ctcctggggg cggatcagta
     1141 tggcatgtat aacctggcat tgagcacagc caatatcgcc attggcttag ctgtattcgg
     1201 gctggattct gccttgattc gctacatcgc cattcaggcc agccgcaagg atgaaaaagg
     1261 ggtatggggt acccttcaag tgggcgtggg tgtggcgacc ttgctgagcg tgatcacagg
     1321 caccttcatt tttggattgg cttattatct ggctgagacc gtattccacg aaccccgcct
     1381 tgtaccattg ttgcaattta caggtgtgat tgttccatta ctgaccatga gcgaggtttt
     1441 agctggggcg ttgcgtggtt tcaagagaat ggattaccct gtcatcgcgc agttcgtctt
     1501 tcagccagtt gttaggctca tcatgatcgt ggtattggca tttactggct tcaatgctgt
     1561 tcatgcgata cttacctttg ggctggctga cttggcagcc tccataatgc ttatttattt
     1621 cctaaacaag gagttttctc tccgccgatc agtgcgagca gcccgacgag acttccgaac
     1681 gatatttgag ttctctattc ctgtttggtt gtcggagatg ttggtcaaat tccacggaaa
     1741 catccagact ctaatccttg gatcattgaa cacaattaca ggcgtaggga tttttagtgt
     1801 tgccagccag atatcaatgg tgagcagcca gttttcctca tcgatcaaca cctcagctaa
     1861 accagtcatc gccgaactgc acgaccgggc agatatgagg gagatggggc agatatatca
     1921 gacggcaaac aagtgggcgg tgatggtgca aatcccgata tttataacaa tggtgctttt
     1981 tccagccccg atcttatcct tgttcggtga aagtttcaca gatggcgcgc ttgcgctttc
     2041 aatcatcgcg ttggctgacc tgttaaaagt gggaacaggt atggggggaa tcatcattga
     2101 tatgaccggt tataccaagc tgaaactggt aaattcgatt ttccggctgg tagccaatct
     2161 gggtttggat atcctgctga tccctcgctg gggtttattg ggtgcagccg tggcagcact
     2221 gattggcgaa ggtttcatta acctactccg tctctttcaa gtgtttttcc tgtttcggtt
     2281 aattccgtac aaccggacct tcatcaaacc ggtcattgct ggtgtaataa cgttagcgag
     2341 tgccttcttg attaaaacct ggcttatctt ggatggggga ttattttttg tgattgcaaa
     2401 tacgggctta ttactagctg tgtatttggg tataacccta ctgatgggat tctctcctga
     2461 ggatcaagcg gttattgggt tcatacggca acgagcaaat aaattgttct caaaaaactc
     2521 ggggaagtaa ttctgaaaac ccctaaaaat ttaggaaatc aaactatggg taaaggacca
     2581 atatttatcg gcgggacaga tcgttccggg aagacgactt tgcgagcatt tctcgtatca
     2641 cacccgaata tttcaatccc cgccaatggt tctaacatgt ggtcgtactt ttatggtcaa
     2701 ttcggcgacc tgagccaggc cgaaaatttt gagcgttgcc ttgacgcgct gatgcactat
     2761 aaacacgcac tttatctcaa gcctgatccc gatcgcatcc gccacgagtt ttggcaaggg
     2821 acaccaacct acgcccgtct gtttgcatta tttcacgaac atctcgctga acgcgaaggc
     2881 aagcctcgct ggggagacca aaccggctta attgaacggt atgcagatca aatatttgag
     2941 gcgtatccaa atgcgaagat gatccaaatg atccgtgatc cacgtgatcg ctaccaggct
     3001 tcgctgactc tctggccaaa gggaaagggg cgggcaggcg gagctacagc cagatggctc
     3061 tattcgacgt ctcttgcaat ccgcaatctg cgcagatatc ctaatcgcta tatgatcgtc
     3121 agatatgaaa cattggtgcg tgataccgag caaaccttgc gtgaggtctg cactttttta
     3181 gatgaggatt ttattccaag catgattacc atggatggcg acctcgatcg tcgatctagg
     3241 ctaacccaag gtttaaacat caagcctgat acgaatccat taacgccaga ctttattggt
     3301 ctttaccgcc aagcagttcc ccaaaaagag atcgccttca tacagggcat tgccggaaag
     3361 caaatgaaag ctatcggtta ctctctggat gcgataagtt tttcactctc agatcgaatc
     3421 agatacattc ttctcgactg gccacttaat atgttgcgca tgttagcatg gttatccctt
     3481 gagatcgcac accataattt tccagctcag ttcggacgta agctaagctc caatatgatc
     3541 accccgcgca aacatcaaaa taacaaggtc aatgcaaggg caacatgatg aagacgatgg
     3601 gtgtaggtga attgttaggg gatcgcgggc cagttttcat aggaggaatc cagcgctctg
     3661 gcaaaacata cttgcgattt atgctctcat cgcattcccc cctggtattc acccgtcgga
     3721 caaacatgtg gccgaatatc tacaatcgtt tcggtgatat cagccagg
//
LOCUS       DBNHOH010000082         1593 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1541829, whole genome shotgun sequence.
ACCESSION   DBNHOH010000082 DBNHOH010000000
VERSION     DBNHOH010000082.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1541829"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="AC16V7_01425"
     CDS             complement(<1..206)
                     /locus_tag="AC16V7_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255806.1"
                     /translation="MRKNLLAIIILLVSLSMVLSACASQATPSPEAEVEAPPEETTEE
                     VTLRVLVHQNPPMVEFIEAFNAKF"
     gene            594..>1593
                     /locus_tag="AC16V7_01430"
     CDS             594..>1593
                     /locus_tag="AC16V7_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR6255807.1"
                     /translation="MESGKNFTIQDIANRANVSISTVSRVLNNTAPVAEETRQRVLSI
                     ITELGYKPNLFAQGLAGGQSRTIGVLTQLIGSPFYDVILRGILRGIDGSGYSPLFADG
                     GWDAEKDQIALYMFMQRQVDGLIVLNGHSPDEFLIEIGLRIPMIIVGREIAELSEQCL
                     PFDDLEAAYKATQHLIDAGHRRIAHITGLPNHQDANERRDGYIKALEQNGIQPDPELI
                     IEGDFTEPSGVMAVEMLLMRGRIFSAIFAANDQLAYGARLALYRRGLRVPEDVSIIGF
                     DDQAPSAYMVPPLTSIRRPPLEIGEAAGRALIYLIQGKSISLPNFQSSLVIRESVSR"
BASE COUNT          376 a          362 c          406 g          449 t
ORIGIN      
        1 atgaactttg cattgaaggc ttctatgaat tccaccatgg ggggattctg gtgcacgagg
       61 accctcagag tgacttcctc agttgtttct tcaggtggcg cttcaacttc tgcctctggg
      121 gaaggtgtag cctggctcgc gcaggcgctc agcaccatgc tcaggctcac tagcagaatt
      181 atgatggcta aaaggttctt tctcatctcg aaatctctcc tttattattg tttagtatct
      241 tgctggatcg cggaattaaa agttactggg gtgtgcaaaa cgttgtattt taggttcacc
      301 tcctgaactc agaccaactg tccacttgga tatatcgatt tgcttatccg tgtgtaacat
      361 cgtgaaactg tccgagaatg aaagcgttat catgtaaatc agatgcaaat tgtctatgaa
      421 tagaaagcgc tttcatgatg gtagcataat ttttatctta agtcaatagg atatggagtg
      481 tattaatcta tttttcctgt gtttacgcca taaagatagg gctgaagttc gtttgcgcaa
      541 tggttgactg aaagcgcttt ctgtgtataa ttgaattgtc aattgaggtg tagatggaat
      601 caggaaaaaa tttcactatt caagatattg ccaatcgagc gaatgtctca atcagcactg
      661 tctcccgtgt gttgaataat actgcgcctg tagcagaaga aactcgccaa agagtgctca
      721 gtatcatcac cgagcttggt tataaaccaa atctctttgc tcaaggcctg gccggcgggc
      781 aatccaggac catcggcgtg cttacccaac tgatcggcag cccattctac gatgtcatct
      841 tgcgtggcat cttacgaggc attgatggca gcggttactc gcctctcttc gccgatggtg
      901 gttgggatgc tgaaaaagat cagattgctt tgtatatgtt catgcagcgc caggtggacg
      961 gtttaattgt tctgaatggt cattcacccg atgaattcct gatcgagatt ggtctccgga
     1021 ttcccatgat cattgtgggg cgagaaattg cggagctaag cgagcagtgt ttaccctttg
     1081 atgatttgga agcggcttac aaagccacgc agcaccttat tgatgcgggc catcgtcgaa
     1141 tcgctcacat caccgggctg ccaaatcatc aggatgccaa tgaacggcgc gacgggtata
     1201 tcaaggctct cgagcagaat gggattcaac cggatccgga gctaatcatt gaaggtgatt
     1261 ttaccgagcc ttcgggggtg atggccgtag aaatgttgct catgcgcggc cgcattttca
     1321 gcgccatctt tgccgccaac gatcagttgg cttatggagc taggctggct ttatatcgca
     1381 ggggcttgcg tgtgcccgag gacgtgtcca ttattggctt cgatgaccag gctccctctg
     1441 cctatatggt accgccattg acttccatcc ggcgcccgcc attggagatc ggcgaggctg
     1501 ccggtagggc attgatctat ctgatccagg gaaaatccat ctcattaccc aattttcaat
     1561 cctcgctggt gatcagggaa tcggtgagca ggc
//
LOCUS       DBNHOH010000083         1921 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1541843, whole genome shotgun sequence.
ACCESSION   DBNHOH010000083 DBNHOH010000000
VERSION     DBNHOH010000083.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1541843"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..133)
                     /locus_tag="AC16V7_01435"
                     /pseudo
     CDS             complement(1..133)
                     /locus_tag="AC16V7_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734807.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
     gene            complement(139..1020)
                     /gene="rfbD"
                     /locus_tag="AC16V7_01440"
     CDS             complement(139..1020)
                     /gene="rfbD"
                     /locus_tag="AC16V7_01440"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009458765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HIR6255808.1"
                     /translation="MRILLLGKNGQLGWELQRTLATLGEVIAVDYPEIDLAQEGAAQK
                     AVRQARPQLIVNASAYTAVDQAESEEELAYAVNARAPGVLAEQAVDLGAALIHYSTDY
                     VFDGSKGSPYIESDTPNPLNVYGRSKLAGEQAIEHIGGAYLILRTSWVYSLRRDSFVT
                     KVLQWSRKQPSLRIVDDQVSNPTWARMLAETTAQLIAKAGGDPSGWLGERRGLYHLAG
                     NGYASRLEWAQAILSHDPHKEEQVTREILPAHTADFPTLAQRPLFSALNCDKFAHTFG
                     LCLPGWEDALRMAMETC"
     gene            complement(1023..1910)
                     /gene="rfbA"
                     /locus_tag="AC16V7_01445"
     CDS             complement(1023..1910)
                     /gene="rfbA"
                     /locus_tag="AC16V7_01445"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HIR6255809.1"
                     /translation="MKGIILAGGRGTRLYPLTIGISKQLLPVYDKPMVYYPLSMLMLA
                     GIREILVISTPEALPAFKALLRDGSQWGLQFSYAEQSEPRGLADAFLVKRGFSTGESV
                     CLILGDNIFFGHGLPSQLRTAAQLTEGALIFAYPVRDPQRYGVVEFDEQGRALSIEEK
                     PIHPRSHYAVPGLYFYDQHVEEIAANLKPSARGELEITDLNRTYLERGQLRVEPLGRG
                     VAWLDAGTHESLLQAANFVQAVEERQGMMISCPEEIAYRMGNITKEELRLQAQSMDTN
                     EYGKYLLQLLDEDIPFHQR"
BASE COUNT          401 a          582 c          528 g          410 t
ORIGIN      
        1 agcgaacggc ttcccttgca atagcttttt cctcaagcca aagctggcgc gctgcctcta
       61 agagtgtaaa tgtgccgacc acgttggtct ggacaaattg ctcagatccc aggatggagc
      121 ggtccacatg cgatgccatc aacatgtctc catcgccatt ctgagcgcat cctcccatcc
      181 aggcaggcat agcccaaagg tatgagcaaa tttatcacaa ttcagcgcag agaacaatgg
      241 gcgttgggcg agcgtgggga aatccgcggt atgggctggc aaaatctcgc gcgtcacctg
      301 ctcctcctta tgggggtcat ggctcaggat ggcctgcgcc cactccagcc ggctggcgta
      361 accgttgcct gccagatggt acaaaccacg gcgctcgccc agccaaccgc tgggatcacc
      421 ccccgccttt gcgatgagct gcgcagtggt ttctgccagc atgcgcgccc aggtggggtt
      481 gctcacttga tcatctacaa tacgcaggct gggctgctta cgagaccatt gcaacacttt
      541 ggtgacaaag ctgtcgcggc gcaggctata cacccaactg gtgcgcaaga taagataagc
      601 tccgccaatg tgctcaatcg cctgttcgcc tgccagcttg ctcctgccgt aaacattcag
      661 cgggttgggc gtatcgctct caatgtacgg gctgcctttg ctgccgtcga agacgtaatc
      721 agtcgaataa tggatgaggg cagcacccag gtcaacagcc tgttctgcca gcacgccggg
      781 tgcccgggcg ttgaccgcat aggccagctc ctcttcgctc tctgcctggt ccactgccgt
      841 ataagcgctg gcgttgacga tcaactgagg tcttgcttgg cgcaccgctt tttgtgctgc
      901 accctcctgc gccaggtcga tctccgggta atccacagcg atcacctcac ccagcgttgc
      961 taatgtgcgc tgtaactccc aaccaagctg tccattttta ccgaggagta gaatgcgcat
     1021 gattaccgct gatgaaaagg gatatcctca tctaacaact gcagcaggta tttgccatac
     1081 tcgttggtat ccatcgactg ggcctgcaga cgcagttctt ctttggtgat attgcccatc
     1141 cggtaggcaa tctcctctgg gcaggatatc atcatgccct gccgctcttc gactgcctgg
     1201 acgaagttag ccgcctgcag cagcgattcg tgcgtgccag catccagcca ggccacgccg
     1261 cgccccaatg gctcgacgcg tagctggccg cgctccaggt aggtgcggtt taggtctgtt
     1321 atctccagtt cgccccgcgc cgagggcttg agattggcgg cgatctcttc gacatgctga
     1381 tcgtagaaat acaaaccggg taccgcataa tgcgaacgtg gatggatggg cttctcctca
     1441 atgctcaagg cgcggccctg ttcgtcaaac tcaaccacgc cgtaacgctg cgggtcgcgc
     1501 accgggtagg cgaagatcag cgccccttcg gtcagctgtg cagcagtcct aagctgggag
     1561 ggcaggccgt gcccaaagaa gatattatcc cccaagatca ggcagactga ctccccagtg
     1621 ctgaagccgc gcttgaccag gaatgcgtcc gccagcccgc gcggttcgga ttgctcggcg
     1681 taagagaatt gcagacccca ttgagatcca tcccggagca gggctttaaa ggccggcaat
     1741 gcctcgggcg tactgataac caaaatttcg cggatgcccg ccaacattaa catggatagc
     1801 ggataataca ccataggctt atcgtagacc ggcagcagtt gcttgctgat gccaatggtg
     1861 agcgggtaca agcgggtgcc gcgcccaccg gcaaggataa tgcccttcat aaattccctc
     1921 c
//
LOCUS       DBNHOH010000084        11621 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_4151, whole genome shotgun sequence.
ACCESSION   DBNHOH010000084 DBNHOH010000000
VERSION     DBNHOH010000084.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11621)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11621)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11621
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_4151"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1581
                     /gene="ptsP"
                     /locus_tag="AC16V7_01450"
     CDS             <1..1581
                     /gene="ptsP"
                     /locus_tag="AC16V7_01450"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIR6255810.1"
                     /translation="AAQKALLELVALLQKVASEVGEEDAAIFDAHQMILRDPDLEEEL
                     RAEIDTGASAESATEVVFARQVKSFEEIDDPLFRERAADFSDVGRRVLCILTGVQDNA
                     LAALDAPAIVIAKDLMPSDTAQMNKAMVRGFCTAGGGPLSHTAILARNLGLPAVVGAG
                     KDVLDLKNGEWLVVDGGAGVVVIGADEQQCNQYQQEADRLAQERATAQAHAHEPAITQ
                     DGRQIEVVANVGLPGEEVDALQAGAEGIGLLRTEFLFVERRVAPSEEEQYQAYYPIFD
                     AMGERIVIARTLDIGGDKPVPFLAVPHEDNPYLGWRAIRIGLADPELLKTQLRALLRA
                     GHNHNLKIMFPMIATVEEMQAARALVDEARAELTERGAPYAQTVDVGTMIEVPSAALM
                     ADQLVRHVDFFSIGSNDLTQYTLAADRGNPAVASLSDALHPAVLRLVDNVVRAAHAAG
                     VWVGVCGEMAGDPNAIPLLLGLGVDEFSAGVAAIPATKALIRKMSLVEAQELAREALA
                     QPGAPAVHALVKRWMGEH"
     gene            complement(1709..2086)
                     /locus_tag="AC16V7_01455"
     CDS             complement(1709..2086)
                     /locus_tag="AC16V7_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR6255811.1"
                     /translation="MPTLNRVQLIGRLGRDPEVRHTPSGKKVTSFSLAVDRRWRSSEG
                     EIKDATDWFQIVVWGRLGEICNQYLSKGRLVYIEGRLQTDRYEHQGETRYFTRVIAAQ
                     MQMLDRKHDEEEAVLEEDDPAIE"
     gene            2253..2918
                     /locus_tag="AC16V7_01460"
     CDS             2253..2918
                     /locus_tag="AC16V7_01460"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HIR6255812.1"
                     /translation="MYIAIEGVIGVGKTTLARLLQPVFQAELLLEVFEENPFLSDFYA
                     DRERYAFQTQIFFLLSRYHQQRNAVPEILKRDMNLLADYTFEKDALFARINIKGDELE
                     MYHRVHEALAEKIPLPDLIVYLRADTDVLMQRIALRDRPYERNMERDYIDQLNRAYED
                     FFVDARASEGHRRSPVLAVDTNQIDFVRRPADLKSMEQRIRQALRLSPFQEELPIKMS
                     SSG"
     gene            2964..3968
                     /locus_tag="AC16V7_01465"
     CDS             2964..3968
                     /locus_tag="AC16V7_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255813.1"
                     /translation="MRMNRDILLKIAEETVSKVVRRDHSVLAAYLCGALLEEDFLLGG
                     TADIDLAFIHLDTPPVEREVQRLTDEIHLDIAHYDQGDFQQARNLRMHPWLGPTIYAC
                     KVMYDPQHFMDFTQASVRGQFYRSDYILGRATQQVEHARQIWLGFASQVKNKPGPRDL
                     ITYLRAIEHAANAVASLDGPPLPERRLLIGFPARAEATGRPGLYPALLGMLGAPQVDG
                     DELKSWLPSWQGALESLPKGAIPARLNPCRHVYYRRSFEEILDGERPADILWPLLRTW
                     TIAAASLSDQEEGYTAWRRACQRLGLLGEGFSERVMALDAFLDLVEETIEDRSRQYGE
                     "
     gene            complement(4001..5458)
                     /locus_tag="AC16V7_01470"
     CDS             complement(4001..5458)
                     /locus_tag="AC16V7_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6255814.1"
                     /translation="MANNKLVLVTGATGYIGGRLAPALIENGYQVRCLVRDPSRLNGR
                     SWSANADIVKGDVLSPETLGDAMQGVEVAYYLVHSMSDGKGFRERDLIAARNFSAAAQ
                     QAGVKRIIYLGGLGDPQTDLSAHLRSRQQTGEALRESDVPVTEFRAGVIVGTGSISFE
                     MIRYLTERLPVMVCPRWVYTRTQPIAIQDMLDYLIAALETPESIGKIIEIGGSDVVTY
                     AEMMTGYARARGLKRWLVPVPVLTPRLSSYWVHLVTPIPAAIAQPLIAGLHNEVVVRD
                     HSARCIFPTIQPITYQEAVQAALDSLEAAQVETTWNDALTTSQNDPTPVLLTTQDGMI
                     LEQRQLLLSLPSEAIFRAFVGLGGKHGWLYWNWAWEIRGMVDRLIGGVGLRRGRRDPE
                     GVRVGDAVDFWRVEAVEPNQSLRLRAEMKLPGRAWLQFQVRPHSNGQTLLQQTAFFAP
                     KGLFGLLYWYALYSIHQLMFSGLIQRIAERAKLFT"
     gene            5882..6769
                     /gene="pdxS"
                     /locus_tag="AC16V7_01475"
     CDS             5882..6769
                     /gene="pdxS"
                     /locus_tag="AC16V7_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal 5'-phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HIR6255815.1"
                     /translation="MEQQIATFAVKKGLAQMLKGGVIMDVVTPEHARIAEEAGAVAVM
                     ALERVPADIRADGGVARMSDPELILQIMDSVSIPVMAKCRIGHFVEAQILEAIGVDYI
                     DESEVLTPADEAHHINKHVFKVPFVCGCRNLGEALRRIGEGAAMIRTKGEAGTGDVVE
                     AVRHARSVLGEIRRLQNMPEEELMTFAKEIGAPYELILETRSLGRLPVVNFAAGGVAT
                     PADAALMMQLGVDGVFVGSGIFKSGDPARRAAAIVKAVTHYNDPYILAEVSRNLGEPM
                     VGRQISSIPESELIAGRGW"
     gene            6763..7359
                     /gene="pdxT"
                     /locus_tag="AC16V7_01480"
     CDS             6763..7359
                     /gene="pdxT"
                     /locus_tag="AC16V7_01480"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal 5'-phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HIR6255816.1"
                     /translation="MVIGVLALQGDFVEHIAMLRRIGAETREVRLPEQLEDLDGLVIP
                     GGESTTIGKLANDFGMLEPLRRFGEQKAIWGTCAGAIFLSKDVHRQQPLLGLMDIKVE
                     RNAFGRQVASFEVDLDVAALRQIAPNGGPYHAVFIRAPLIESVSGDAQILARLDDGRI
                     VAAQQGRLLATSFHPELTRDDRFHRYFLHLVGTMVERL"
     gene            7444..8427
                     /locus_tag="AC16V7_01485"
     CDS             7444..8427
                     /locus_tag="AC16V7_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255817.1"
                     /translation="MNIRPFDWRDLPALHRYRQQSVFLHSSLVLTRGPMLISSALLSS
                     LAPNMGISTSVSVEEGDQDQKLIGQSLHSLGSQCAQLTFLTPDGALDSDNLSSLLEHL
                     SKQAVERGAFRLLADVDENTQAFEALHQAGFAIYARQRIWQLKGSPTMPSVSAGWRHA
                     SDRDLIPVRSLYANIIPGLVQQIEPFPAERLNGLIYRQGDELLAYVELKYGHRGIWAQ
                     PFVDPDLKDVTSRLAELLSNLPNRLSRPVYLCVRSYQAWLESTIEELGAEPSQRQAMM
                     VKHLAIPQKALRAVSLPNLEGGHPEVTAPIRQTQAVSPQNGGRAAAQLENH"
     gene            8433..10052
                     /locus_tag="AC16V7_01490"
     CDS             8433..10052
                     /locus_tag="AC16V7_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HIR6255818.1"
                     /translation="MSQRRITDDLQALLDVLPQSISQAVSDANDSDNLLEVILDLGRV
                     PTARFVDREVVLSPTEVPHKDIDYVVERIGAFDADNRAGLERTLHRISAIRNRRGHIV
                     GLTCRVGRAVYGTTDIIKDLIEGEKSLLLLGRPGVGKTTMLREAARLLAESKRVVIVD
                     TSNEIGGDGDVPHPAVGRARRMQVSTPSLQHEVMIEAVENHNPEAIIIDEIGRELEAA
                     AARTIAERGVQLIGTAHGNTLENLLLNPTLSDLVGGIESVTLSDEEARRRGTQKTVLE
                     RRSPPTFDVLIEIQTRQRLAVHEDVAASVDALLRGFPLSPEIRSRDESGSIQIEKPVQ
                     PARAYSQQQGLRRQHTNGGRAYSPQAIRDEYPAESQPPAYTAEDRASRGPLQTVRIYA
                     YGVARNRLSQAARRLSVPVVLVNDPGQADVLVTLRSYYRKRQRTVVDAENRGMPIYVL
                     RANTINQMLQFLMGLFNLAEEQPDEMGEDRIRQETMAAISAVLNGERWVELSPASSGV
                     RRLQHQMARQANLLSHSYGKEPRRRVRIFRE"
     gene            10146..10760
                     /gene="tmk"
                     /locus_tag="AC16V7_01495"
     CDS             10146..10760
                     /gene="tmk"
                     /locus_tag="AC16V7_01495"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR6255819.1"
                     /translation="MFITLEGPDGSGKSTQAGLLVNFLRQRGSSVLATREPGGTAIGD
                     QVRSVLSNLENTAMHPRTEILLFQASRAQLVEQVIRPQLARRGIVVCDRYADSTLAYQ
                     GYGHQVDMVRLRAIVEFATGGLKPDLTLLLDIDVEEGLRRRTKSGEWNRLDAYDLAFH
                     QRVRQGYFQLAQDEPQRWVKIDAAQPPVMVQADIQRVVLERLEA"
     gene            10994..11512
                     /locus_tag="AC16V7_01500"
     CDS             10994..11512
                     /locus_tag="AC16V7_01500"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6255820.1"
                     /translation="MKIPQLATSRLTLRAFTDDDVVPFHRMLIEKDVLRYFPNTDPPS
                     LERVQKMVSGILKHWAEHGFGLWAVERRQSGEFMGRCGLQVIPDTGEVEVDFILGKAF
                     WGQGYATEGGQVSVQYGFERLGVEMIVGIVHPENMASQRVLEKLGLVFREKTQYFGMD
                     CFRYAVENQVEP"
BASE COUNT         2478 a         3108 c         3447 g         2588 t
ORIGIN      
        1 gcagcgcaaa aggctttgct agagctggtt gccttactcc agaaagtggc ttctgaggtg
       61 ggcgaggaag acgccgcaat ttttgatgct caccagatga tcctgcgcga tcccgacctg
      121 gaagaagagc tgcgcgccga gatagacaca ggcgcttcgg cagaatctgc caccgaagtg
      181 gtttttgccc ggcaggtaaa aagtttcgaa gagattgacg acccgctgtt tagagagcgc
      241 gcagctgact tttccgatgt gggccggcga gtgctgtgta tcctcaccgg cgtgcaggat
      301 aatgcgctcg ccgctctgga tgcacccgcg attgtgatcg ctaaggacct gatgccctca
      361 gacacggccc agatgaacaa ggcaatggtg cgcggttttt gcacggcagg cggggggccg
      421 ctgtcacata ccgccatcct ggcgcgcaac ctcggtctgc cagcagtagt tggcgcaggc
      481 aaagacgtgc tcgacctgaa aaatggggag tggttggttg tagatggggg tgctggggtg
      541 gtggttatcg gggcagacga gcagcaatgc aaccagtatc aacaggaggc ggacaggctg
      601 gcacaggagc gcgctactgc gcaagcgcac gcgcatgagc ccgccatcac gcaggatggg
      661 cggcagattg aggttgtcgc taatgttggc ctgcccggtg aagaggttga cgctctgcag
      721 gctggcgctg aaggcatagg tttgttgcgc accgagttcc ttttcgtcga gcgccgggtt
      781 gcccccagcg aagaagaaca atatcaggca tactatccga tctttgatgc catgggggag
      841 cgcattgtta tcgcccgcac cttggacatc ggcggcgata aaccggtgcc atttctggct
      901 gtgccccacg aggacaatcc ctatcttggc tggagagcca ttcgcattgg cttggccgac
      961 cccgaactgc tcaaaacaca gttgcgggct ttgctgcgcg ccgggcataa ccacaaccta
     1021 aaaattatgt ttccaatgat cgctacggtg gaggagatgc aggcagcgcg tgctctggtc
     1081 gacgaggcgc gcgccgagct gaccgagcga ggggcgcctt atgcccaaac ggtcgatgtg
     1141 ggcacgatga tcgaggtccc ctctgcagcg ctgatggccg accaattggt acggcacgtg
     1201 gatttcttta gcattggcag caatgacctg acccagtata ccctggcagc agatcgcggc
     1261 aatccggcgg tcgcctcgct ttccgacgct ttgcacccgg cggttttacg gctggtggac
     1321 aatgtagtcc gcgccgccca cgctgccgga gtatgggtgg gtgtgtgcgg cgagatggct
     1381 ggcgatccaa atgcgatacc gcttctcctt ggcttggggg tggacgagtt cagcgctggg
     1441 gtggctgcta ttccggcaac aaaggcgctg attcgcaaga tgtcgctggt cgaagcgcag
     1501 gaattggcac gtgaagcgtt agcgcaaccc ggtgccccag ctgtgcatgc gctggtgaag
     1561 cgttggatgg gggagcattg atcaaagggc aataaaacaa cttgtgaaaa ttatcacaaa
     1621 tagactataa ctgatgaaag ccaatcacca ccctcctgag gaggcaaatg attggctttt
     1681 tcggtagttc cccacacatt ttgagctctt attcgatcgc cgggtcgtct tcttccagga
     1741 ctgcttcctc ttcgtcgtgc ttgcgatcga gcatctgcat ctgcgcggcg atgacgcggg
     1801 tgaagtaacg tgtctctccc tggtgttcgt aacgatcggt ttgcaggcga ccctcgatat
     1861 agaccagcct ccctttgctc aggtactggt tgcatatctc ccccaggcgt ccccagacca
     1921 caatttggaa ccaatcggtg gcgtctttga tctccccttc gctgctgcgc caacggcgat
     1981 cgaccgccag gctgaagctg gtgaccttct tgcccgaagg tgtatgtcgc acctccggat
     2041 ctctgcccaa acgtccgatc aactgtacgc ggtttagagt aggcatgtct ttcctcctta
     2101 gatggtgtct ttctccagtt tagcgcgggt ttgcgtaaag tcaagaaaat ggtgtgagtt
     2161 aagaattgcg gggaaattgt tcgaatctgt tgcgcactcc gccaatcgct ggtatcctta
     2221 ccacaaccat catctcacaa gggaatcata atatgtacat cgcaatcgaa ggcgtcattg
     2281 gcgttggcaa gaccaccctg gcccgcttat tgcagccggt attccaggct gagctgctcc
     2341 tggaggtctt tgaagagaac ccgttcctct cagatttcta cgctgaccgt gagcggtatg
     2401 cctttcagac ccagattttc ttcctgctca gccgctacca ccagcagcgc aatgctgtcc
     2461 ccgagatact caagcgggac atgaacctgc tggcagatta tacgtttgag aaagacgcac
     2521 tgtttgcccg tatcaatatc aaaggagacg agctggaaat gtaccatcgg gttcatgaag
     2581 cgctggctga gaaaatccca ctgccagatt tgattgttta tctgcgcgct gataccgatg
     2641 tgctcatgca acggattgct ctgcgcgacc ggccttatga gcggaatatg gagcgtgact
     2701 acatcgacca gcttaaccgt gcttatgagg acttttttgt cgatgcgcgt gccagcgagg
     2761 ggcaccggcg atccccggtg ctggcggtcg atacgaatca gatcgacttt gtaaggcggc
     2821 cggcagatct gaaatcgatg gagcagcgta tccgccaggc gctacgtcta tcaccattcc
     2881 aggaagagct tccgataaag atgtcatcga gtggatgatg cgagataggg gagcgcagtg
     2941 gagcgagtca ggagaattaa tctatgcgca tgaatcgcga catcctgctg aagattgccg
     3001 aagagacggt ttccaaggtt gtccgccgag atcatagcgt cctggctgct tacttgtgcg
     3061 gggctttgct ggaagaagat tttctgctgg gcggcacggc agatatcgat ctggctttta
     3121 ttcatttaga tacgccccct gtcgagcgcg aagtgcagcg cctgactgat gaaattcatc
     3181 tggacattgc ccactacgat cagggtgatt ttcaacaggc gcgtaatttg cgtatgcacc
     3241 cctggcttgg gccgacaatc tacgcctgta aggtgatgta tgacccccaa cacttcatgg
     3301 atttcaccca ggccagtgtg cgcggacagt tttaccgctc ggattacatc ctgggtcgcg
     3361 ctacccagca ggtggagcat gcccgccaga tctggttggg tttcgccagc caggtcaaaa
     3421 ataagcctgg gccacgcgat ttgatcacct atctgcgcgc catagagcac gctgcgaacg
     3481 cggtcgccag cctggacggt cccccgctcc cagaacggcg attgctgatt ggttttcctg
     3541 cgcgcgccga ggcaaccggg cgacctggcc tgtaccctgc cttgctgggg atgttgggtg
     3601 cgccgcaggt ggatggcgat gagcttaaat cttggctacc cagttggcag ggcgccctgg
     3661 agtcgctgcc caagggggcg atccctgcca ggttgaatcc ctgccgccat gtttactacc
     3721 ggcgttcgtt tgaagagata ttggatgggg aaaggccagc cgatatactc tggcccttat
     3781 tacgcacgtg gacgatcgct gccgcatcgc tctcggatca ggaagaaggg tacactgcct
     3841 ggcgaagagc ctgccagcgc ctgggtttgt tgggagaggg gttcagcgaa cgggtaatgg
     3901 cgctggacgc ctttttagat ctggttgaag agacgataga ggatcgctcg cgccaatatg
     3961 gggagtaggt tggcgttcca atgtttcacg tgaaacatcc ttaggtgaag agcttggctc
     4021 gctcggcaat tctctggatt agaccgctga acattaattg gtggatcgag tacagagcgt
     4081 accaatagag cagaccaaat aagcctttcg gcgcaaaaaa tgctgtttgc tgtagcaagg
     4141 tttgaccgtt ggaatgaggt ctaacctgga attgcagcca ggcacgccca ggcagcttca
     4201 tctcggcgcg caggcgcagg gattggttag gctcaaccgc ctccacccgc cagaaatcga
     4261 cagcatcccc cacgcgcacg ccctccggat cacggcgacc gcggcgcaaa ccgacaccgc
     4321 cgatcagacg gtcgaccatg ccgcgtattt cccaggccca attccagtat agccagccgt
     4381 gcttgccgcc caggccaacg aaagcccgaa aaatagcctc tgaaggaagc gacagaagca
     4441 gctggcgctg ctctaaaatc atcccgtctt gggtggtaag gaggaccggg gttgggtcgt
     4501 tctggctggt agtcagggca tcgttccagg ttgtctcaac ttgggcggcc tccaggctat
     4561 cgagtgcagc ttggacggct tcctggtaag tgatgggttg aatagtcggg aagatgcacc
     4621 tagcagagtg gtctcgcacg acgacctcgt tgtgcagccc ggcaatcaac ggttgtgcga
     4681 ttgcagctgg tattggggtg accaggtgca cccaatagga agagaggcgt ggggtcagta
     4741 ctggtacagg taccagccag cgcttcaaac cgcgcgccct ggcatagccg gtcatcatct
     4801 ctgcgtaggt cacgacatct gacccaccga tctcaatgat ctttccgatg ctctccgggg
     4861 tttccagagc cgcaataagg taatccagca tgtcttggat agcaatcggc tgggtgcgtg
     4921 tgtagaccca gcgcgggcag accatgacag gtaagcgctc ggtcaggtag cggatcattt
     4981 caaatgaaat gctgccagtg ccaacaatca cgccggcgcg gaactcggtc actgggacgt
     5041 cgctttctct caaggcttcc ccggtttgct ggcgcgagcg caggtgggca gagagatctg
     5101 tttgcgggtc gcctagaccc ccaaggtaga tgatgcgctt tacgccagcc tgttgggcgg
     5161 ctgctgaaaa gttgcgcgcc gcgatcaggt cccgctcgcg gaaacccttg ccatccgaca
     5221 tgctgtgaac caggtaatag gccacttcca caccttgcat cgcatcgccg agcgtttcgg
     5281 gggagagcac atcacctttt acgatgtcgg catttgccga ccaggagcgc ccgttcaggc
     5341 gggaggggtc gcgcaccagg cagcgtacct ggtatccatt ttcaataagc gctggcgcca
     5401 aacgcccgcc gatatagccg gttgcgccag tgacgagtac gagtttgtta tttgccattt
     5461 gatttcctct tatgcgagat ctctattttc aggacaggtc tttgcagcca cagcgataat
     5521 catctcagac taaatgcgat attgtttacc caaatccata taaaatatac tacaaagtca
     5581 cgtttttgtc aatgtattat agattatttg tgtaaaaaat atatacaatt gttctttggc
     5641 gattatactg cctatgatct gaactatttt tcccctctcg tggtgctaca cttgctgctc
     5701 ccaggagagc accggcatac atgggtagtc cgcttgaccc gactggaata cttaatttca
     5761 tccatccagt gacccaactg gcgcgatcga gttgctctaa aagtggcgga ttgcattgcc
     5821 tatggctgcg tggtagaatg ttgaaaaaac cgagtcgaat tcgactcagg aggtctggta
     5881 tatggagcag caaatcgcaa cttttgccgt taaaaaaggg ctagctcaga tgcttaaagg
     5941 tggcgtgatc atggatgtgg ttaccccgga gcatgcacgc atcgccgagg aagccggcgc
     6001 tgtggcggtc atggcgctgg agcgcgtccc ggcggacatc cgcgccgatg ggggtgtggc
     6061 tcgcatgagc gacccagaat tgatcttgca aattatggac tccgtctcga tcccggtaat
     6121 ggcgaaatgc cgtatcggtc acttcgtaga ggcgcagatc ctggaagcta tcggtgtgga
     6181 ctatattgat gaatcggaag tgcttacccc cgccgatgag gcccatcaca ttaacaagca
     6241 tgtttttaag gtgccatttg tttgtggctg ccgcaatctg ggagaggctc tgcgccgcat
     6301 cggcgagggt gcagccatga tccgcaccaa aggcgaggcc ggcactggcg acgtggttga
     6361 agccgtgcgc catgcccgct cggtattggg tgaaatccgc cgcctgcaga atatgcccga
     6421 agaggaattg atgacctttg ccaaggagat cggcgctccc tacgagctga tactggagac
     6481 tcgcagcctg ggtcgccttc cggtagtgaa ttttgccgcc ggaggtgtgg ctacacctgc
     6541 agatgctgcc ttgatgatgc aattaggcgt ggacggtgtg tttgtcggct cgggcatctt
     6601 caagtctggc gatccagccc ggcgcgcggc cgccatcgtc aaagccgtga cgcactataa
     6661 cgacccttat atcctggctg aggttagccg caacctgggt gagccgatgg taggcagaca
     6721 aatctccagc attccagagt ctgaattgat cgctgggcgc ggatggtgat cggagttctt
     6781 gcgcttcagg gcgattttgt tgaacacatc gcaatgcttc gccggatagg ggctgaaacg
     6841 cgagaggtcc gtcttcctga gcagctggaa gacctggatg ggcttgttat ccctggtggc
     6901 gaatcgacca cgattggcaa gctggcgaac gactttggca tgctagagcc gttgcgcaga
     6961 tttggcgagc aaaaagcgat ctggggtacc tgcgcagggg cgatatttct ctccaaagat
     7021 gtccatcggc agcaaccttt attgggcttg atggacatca aggtggagcg caacgccttt
     7081 ggacggcagg ttgccagctt tgaagtggat ttggatgtgg ctgcgctgcg acagatagcc
     7141 cccaatggag ggccatatca cgccgtattc atccgcgccc cgctcatcga gtctgtttcc
     7201 ggcgatgccc agattctggc gcgcctggac gacgggcgta ttgtcgctgc gcagcaaggg
     7261 cgcttgctgg cgacctcttt tcatccagag ctaaccagag atgaccgttt tcaccgctat
     7321 ttcctacatt tagtgggtac aatggttgaa agattatagg tggaaggttt ataatggttg
     7381 caggttatta ggttgaaggt tcagtgtacg ctcttcaacg ttcaacctgt aactaacatc
     7441 taaatgaata ttcgtccgtt tgactggcgt gatctgcctg cactgcaccg ctatcggcag
     7501 cagagcgtgt ttctacatag ctcgctggtt ttgacgcgtg gaccgatgct catttccagt
     7561 gcattgttgt ctagcctcgc gccaaacatg gggatttcta cctcggtgag cgtggaagaa
     7621 ggcgatcaag accaaaagct catcgggcag tccctccatt ctttaggatc gcaatgtgcc
     7681 cagttgacct tcctgacccc tgatggggct ctggattcgg acaacttgtc atccctgttg
     7741 gagcaccttt cgaaacaagc cgtcgagcgc ggcgcgtttc gcctgttagc cgacgtggat
     7801 gagaataccc aggcatttga ggcgctgcac caggccggtt ttgccattta tgcgcgccag
     7861 cgaatctggc aattgaaggg aagcccgacc atgccgtctg tttctgcagg ctggcgccat
     7921 gcttcagata gggatctcat cccggtgcgc tctttgtatg ccaacatcat tccgggactg
     7981 gtgcaacaga ttgaaccctt ccccgctgag cgtctcaacg gcctgatcta ccgtcaagga
     8041 gacgaactgc tggcttatgt ggagctcaag tatgggcata gaggtatctg ggcgcagccg
     8101 tttgtcgacc ccgatctaaa agatgtgacc agtaggctgg cagaactgct gtcaaacctg
     8161 ccaaaccgtc tctcccggcc ggtatatttg tgcgtgcgtt cataccaagc ttggctggaa
     8221 tcgaccattg aggagctggg agcagaaccc agccagcgcc aggcgatgat ggtcaaacat
     8281 cttgccatcc cccagaaagc gctacgcgcc gtgtcgctgc cgaacctgga aggcggccac
     8341 cccgaggtga ccgcaccgat taggcaaacc caggctgtat cacctcaaaa cgggggacgg
     8401 gcggcggcac aattggagaa ccattagata ttatgtcgca aagacgtata actgacgatt
     8461 tacaggcttt actcgacgtt cttccccaat cgatttcaca agcagtatca gatgcgaatg
     8521 atagcgataa cctgctggag gtcatcctag atctggggcg cgtgccgact gcgcgttttg
     8581 tggatcgtga ggtggtgctc agcccgactg aagtacccca caaggacatc gactatgtcg
     8641 tcgagcgcat tggcgcgttc gatgcggata accgcgccgg gctggagcgt accctgcacc
     8701 gcatttctgc tattcgcaac cgccgtggac acattgtcgg gctaacctgc cgtgttgggc
     8761 gcgctgtcta tggcaccact gacatcatta aggacttgat cgagggtgag aagagtctgc
     8821 ttctgttggg acgccccggc gtgggaaaga ccaccatgtt acgtgaggct gctagattgc
     8881 tggcggagag caagcgcgtg gtgattgtgg atacctccaa cgaaatcggc ggcgatggcg
     8941 atgtgcctca cccggctgta ggccgtgccc gccgcatgca ggtcagcaca ccctccttgc
     9001 agcacgaggt gatgatcgaa gcggtggaga accacaaccc ggaggcgatc attatcgatg
     9061 agatcgggcg cgaattagag gcggctgccg cgcgcacgat cgcggagcgc ggcgtgcaat
     9121 taatcggcac tgctcacggc aacactctgg agaacctgct gctgaaccca accctctccg
     9181 acctggtagg gggtatcgag tcggtcacct tgtccgacga ggaagcccgc cggcgtggca
     9241 cccaaaagac ggttttggaa cggcgctcgc cgcccacctt cgatgtgctc attgagatcc
     9301 agacccgcca gcggttggcg gtgcacgagg atgtagccgc ctctgtggat gccctgctgc
     9361 gaggtttccc actatcacca gagattcgct cgcgagatga gtctgggagc atccagattg
     9421 agaaacctgt tcagccggcc agggcctatt ctcaacagca aggtttgcgc cgccagcata
     9481 ccaatggcgg acgagcttat tcaccccaag cgattcgcga tgagtacccg gctgagtctc
     9541 aaccacctgc ctacaccgct gaagatagag caagccgcgg cccgttacag accgtgcgca
     9601 tctacgccta tggcgtggcg cgtaatcgct tgagtcaggc tgcccgccgc ttgagcgtgc
     9661 cggtggtgct ggttaacgac cccgggcagg cggacgtgct ggtcacgctg cgctcttatt
     9721 atcgcaagcg ccagagaaca gtagtggatg ctgagaaccg cggcatgccc atctatgtgt
     9781 tgcgggccaa cacgatcaac caaatgctgc agttcctcat gggcttattc aacctggctg
     9841 aggaacagcc agacgagatg ggtgaagatc gcatccgcca ggagaccatg gcggcgatca
     9901 gcgctgtgct gaacggggag cgctgggtgg agctgtcacc ggcctcgtct ggggtgcgcc
     9961 gactgcaaca ccagatggct cgccaggcta acctgctctc gcattcttac ggaaaagagc
    10021 ctcgccgccg ggtgcgcatc ttccgcgagt agcttggggt gcgcattgtc catgcaaagc
    10081 tccattttcc ggggtgttct cttcttacct tccctagtaa gatgaagagg gggttggagg
    10141 gataggtgtt tattaccctt gaaggtcctg atggcagcgg aaaatccacc caggcgggcc
    10201 tgctggtcaa cttcttgcgc cagcgcggct ccagcgtgct ggccacgcgc gagccaggag
    10261 gcaccgccat tggcgatcag gtgcgctcgg tgctctccaa tctagagaat acagccatgc
    10321 atccgcgcac cgaaatcttg ctcttccagg cctcgcgcgc ccagctcgtt gagcaggtta
    10381 tccgcccaca actggcccga aggggtattg tggtctgcga tcgctacgcc gattcgaccc
    10441 tggcttacca gggatatggc caccaggtcg acatggtgcg cctgcgcgcc attgtggagt
    10501 tcgctaccgg gggcttgaag cccgacctga ccttgttgct ggatattgat gtagaagaag
    10561 gcttgcggcg gcgcaccaaa agcggcgagt ggaatcgcct ggacgcgtat gacctggcct
    10621 tccaccagcg cgtgcgtcag ggttacttcc agttggccca ggacgagccg caacgttggg
    10681 tgaaaatcga cgccgctcag cctcccgtaa tggtgcaggc agatatccaa agggtggttc
    10741 tggagcgatt ggaagcctag aaaaatagaa gctgtagcgc tagcatgcac ttagggatac
    10801 gtcgggggat gcaatttatt gactttgcct gcaagatttt gtaaactgaa tatgggccgg
    10861 acaatctgaa acgttaaatt atagaaaatc caacccaaat acacatttcc tttgaatcag
    10921 gcatgggact gggtagctca ggtcaagatt gattgggcag tttcctccga tacgcaggaa
    10981 agcgccaacc cacatgaaaa taccgcagct ggcaacctcg cgattgacgc ttagagcatt
    11041 taccgacgac gatgtcgtcc cttttcatcg catgctgatc gaaaaggatg tcttgcgtta
    11101 ttttcccaat accgatccac cttcgctgga gcgggtgcag aagatggtct ccggcatact
    11161 gaaacattgg gctgaacacg gctttggcct gtgggcggtt gaacgtcgcc agagcggtga
    11221 attcatgggg cgctgtggac tgcaggttat cccagataca ggtgaggtag aggtggattt
    11281 tatcttgggc aaggcctttt gggggcaagg atatgccacg gaagggggac aggtgagcgt
    11341 gcaatatggc ttcgagcgat tgggtgtgga gatgattgtg ggcattgtgc atccagaaaa
    11401 tatggcctcc cagcgcgtgc tcgagaagct gggattggtg tttagggaga aaacgcaata
    11461 cttcggcatg gattgttttc gttacgcagt agagaatcaa gtggaaccat gaaccacaaa
    11521 atctttgagg cgagcgaggc gcttatggga ctagacgaaa ctgtagccgt agtccgaccg
    11581 gatccacccg gtcggctgct cttcgtggac aacatccgca c
//
LOCUS       DBNHOH010000085         1429 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1093825, whole genome shotgun sequence.
ACCESSION   DBNHOH010000085 DBNHOH010000000
VERSION     DBNHOH010000085.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1429)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1093825"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="AC16V7_01505"
     CDS             complement(<1..250)
                     /locus_tag="AC16V7_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009782982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255821.1"
                     /translation="MQKFPDSVIPTVVQTRFRTPQLPLRSIANFIVLTSLFAIAYSQS
                     PLYTSNQNQYFLHGYASAGYGNLSRDWLANTLDPTPIFS"
     gene            343..708
                     /locus_tag="AC16V7_01510"
     CDS             343..708
                     /locus_tag="AC16V7_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255822.1"
                     /translation="MKDHIRIIAWLYIILGVLGIFGALIAFAIIAGGGMISGDETAIY
                     VTGIVALVIAVIVVLVSVPGIIAGLGLLKFKPWARILAIILALLNLPGFPIGTALGIY
                     TLWAMLDSESSQLFNVIEG"
     gene            complement(771..1178)
                     /locus_tag="AC16V7_01515"
     CDS             complement(771..1178)
                     /locus_tag="AC16V7_01515"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6255823.1"
                     /translation="MNTVNLPAEYKSLEKISKFVIQAAREAGLSDQDIYEVELAVDEA
                     CSNIIDHAYGGEGIGDMRCSVEVGKGTLKIILNDHGRPFDLALVPEPVVNIPLQKLKR
                     RGVGVYLMRKMVDELHYESSKEAGNVLVLIKRK"
     gene            complement(1185..>1429)
                     /locus_tag="AC16V7_01520"
     CDS             complement(1185..>1429)
                     /locus_tag="AC16V7_01520"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR6255824.1"
                     /translation="LKSITDSGRFKIVFDMSGVTFMSSKGWWVLIDTQKTCKRYKRGE
                     LVLVNVRDEIQDSLNLVGMGSYFNIFTDLTSAVGSF"
BASE COUNT          379 a          319 c          330 g          401 t
ORIGIN      
        1 tgctgaagat aggcgtggga tccagagtgt tggctagcca gtctcgggac agattcccat
       61 aacctgccga ggcgtatccg tgcaagaagt attggttttg gttagatgtg taaagagggg
      121 attgggagta tgcaatcgca aacaggctgg tcagcacgat aaaatttgcg atggagcgaa
      181 gaggcaattg tggagtacga aagcgcgttt gcacaactgt tggtataacc gaatcgggga
      241 atttctgcaa ggcttgtgtc catccaaggt atgcaacaaa gtcgtataga ttacgtaaat
      301 agtatcagga acacaaaact gggtcacata tggaggatca acatgaaaga ccacatcaga
      361 attatcgcct ggctatatat tattttaggg gtgctgggaa tatttggggc gctgattgcc
      421 tttgctatca tagctggcgg gggaatgatc tctggcgatg aaacagccat ctacgtgaca
      481 ggaattgtcg ctttggtgat tgctgtgatc gtggtgcttg tatctgtccc cggcatcatc
      541 gctggattgg gattgcttaa attcaagcct tgggcgcgta tcctggcgat tatcctggct
      601 ttactgaatc taccagggtt ccctatcggg acagcgctgg gcatatacac gctttgggcg
      661 atgctggata gcgaatcttc tcagcttttc aatgtaatag aaggttaaat caagagagca
      721 aaaccggtgt tgtatatcat tacagcaaca attctggttt tgctctcact ctatttacgc
      781 tttatcaaaa ccaatacatt gccagcttct tttgaagact cgtagtgtag ttcgtcaacc
      841 attttgcgca tcaggtaaac acccaccccc cgtcttttta acttttgcaa ggggatattt
      901 accactggtt cgggtactaa ggcaaggtca aaaggtcgcc cgtgatcatt gagaatgatt
      961 ttcagcgttc ctttgccaac ctcaaccgaa caacgcatat cgcctatgcc ttcaccaccg
     1021 tatgcgtggt cgatgatatt actacaggcc tcatctaccg ctaactcgac ttcgtaaata
     1081 tcttggtcac taagacccgc ctcacgggct gcttgtatga caaatttgct gattttttcc
     1141 aggcttttgt attcagccgg caaattaact gtattcatta actcctaaaa gctaccaacc
     1201 gcggaggtga gatcagtaaa aatgttaaaa tacgagccca tccctactaa atttagagaa
     1261 tcttggatct cgtcccgtac attgaccagt accaattcgc cgcgcttata ccgtttgcag
     1321 gttttttgtg tatcaataag cacccaccaa cccttactgg acataaaggt tactccactc
     1381 atatcaaata caatcttaaa ccgacccgaa tcggtgatag attttagcg
//
LOCUS       DBNHOH010000086         2833 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1606232, whole genome shotgun sequence.
ACCESSION   DBNHOH010000086 DBNHOH010000000
VERSION     DBNHOH010000086.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1606232"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2552
                     /gene="ppdK"
                     /locus_tag="AC16V7_01525"
     CDS             <1..2552
                     /gene="ppdK"
                     /locus_tag="AC16V7_01525"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HIR6255825.1"
                     /translation="WEQELEALKQVEAQTGKKFGDPTRPLLVSCRSGAKFSMPGMMDT
                     VLNIGLNDEVAEGMAKLTGDERFAFDAYRRLVEMFGEVVLGIPDEKFEHPMAEYKEKK
                     GVKADTELTAEDWKAITETFKKVVRENRSFDFPQDPIEQLRLATEAVFKSWNGKRAVD
                     YRNAAGIAHDLGTAVNIVTMVFGNMGWNSGTGVAFTRDPANGEKKMYGDYLLNAQGED
                     VVAGIRNTEMITNLANVLPEAYQQFLEICDKLELHYKEMQDVEFTIEQGKLWMLQTRS
                     GKRTAKAAVKIAVDMANEGLISKQEAILRVTPDNVDTLLHPQFDEAAKKSAAVDGRLY
                     ATGVNASPGAAVGQVYFDADTAEHMAKENKQDVIMVRPFTKPDDVHGMLASKGILTSE
                     GGATSHAAVVARQFGVPCVVGAAMVRINLDKREMVVNGQVVREGEWISVDGTTGEVFL
                     GHIPTTSPTLEEQTDLLTLLHWADEICATPGIRSAPDGWPTTGLQVWANADYPKDARR
                     ARSYGAVGIGLCRTEHMFFEPERLPIVQRMILAETSETRKAALDELLPSQRTDFEGLF
                     EAMDGYPVIIRLIDPPLHEFLPSEEELLEEVITMRVKGENKGLAEKEKLLASVKSMHE
                     SNPMMGLRGVRLSIQMPEIVEMQVRAIFEAAANMTIKGVVVKPEVMIPLTGTINELRW
                     IQPRLEKIAKAVMDEKQITFSYKFGTMIEIPRAAVTATEIAELAEFFSFGTNDLTQMT
                     FGYSRDDAERNFLVTYVEQGILSNNPFQTLDRGGVGRLMDLAVKDGRQTRPDLEVGIC
                     GEHGGDPSSIEFCHTIGNNYVSCSPFRVPIARLAAAHSALKHKGVSIAEDK"
BASE COUNT          710 a          726 c          779 g          618 t
ORIGIN      
        1 tgtgggagca ggagctagaa gcgttgaagc aagtggaagc tcaaaccggc aagaagttcg
       61 gagatcccac gcgtccccta ttggtctcat gccgctcggg agcaaaattc tccatgccgg
      121 gcatgatgga cacagttctt aacattggac taaacgacga ggtagcagag gggatggcga
      181 agctaaccgg agacgagcgt tttgccttcg acgcctaccg tcgcttggtg gaaatgtttg
      241 gtgaagtggt tctcggaatt cccgacgaga agtttgaaca tcccatggcg gaatataaag
      301 agaaaaaggg cgtaaaagcc gatacggaac tgactgccga ggattggaaa gccatcaccg
      361 aaacctttaa gaaggtggtg cgcgaaaata ggagctttga ctttccccag gatcctatcg
      421 agcagctacg cctggcgacc gaagccgtct ttaaatcttg gaatggcaag cgcgccgtgg
      481 attaccgcaa cgcagctggc atagcccacg acttgggcac cgcggttaat atcgtcacca
      541 tggtttttgg aaatatgggc tggaattcgg gcacgggtgt ggcgttcact cgcgatccag
      601 ccaatggtga aaagaagatg tatggtgact atttgctcaa cgctcaaggt gaggatgtgg
      661 ttgcgggcat tcgcaatact gaaatgatta cgaatttagc caatgttttg ccagaagcgt
      721 accagcaatt tttggagatc tgcgacaagc tggagctgca ttataaagaa atgcaggacg
      781 tggagttcac catcgagcaa ggaaagttgt ggatgctcca gacgcgaagc ggaaaacgta
      841 ctgcaaaagc cgccgtcaaa attgctgtag acatggcaaa cgaggggctg atttcaaaac
      901 aggaggctat cctgcgcgtc accccagaca acgtggatac cttgcttcat ccccagttcg
      961 acgaagcagc caaaaaatca gccgcagtgg atgggcgcct gtatgccacg ggtgtgaatg
     1021 cttcacctgg ggctgcagta ggccaggtat acttcgatgc cgacactgct gaacacatgg
     1081 caaaagagaa taaacaggat gtcatcatgg tacggccttt caccaagcca gacgatgtgc
     1141 atggcatgct agcttccaag ggcatcctta ccagtgaagg tggggctacc tcccacgccg
     1201 cagtggtggc gcgacaattt ggcgtgccat gtgtggttgg ggctgcaatg gtccgcatca
     1261 acctggataa gcgcgagatg gtggttaatg ggcaggtcgt gcgcgagggt gaatggatct
     1321 ctgtagatgg cacgactggc gaagtgttcc ttgggcatat ccccaccaca tctcccacct
     1381 tggaggagca gaccgatcta ctcacgcttc tccactgggc tgacgagata tgcgccacac
     1441 ctggcatccg ctccgcccct gatggctggc ccacgactgg cttgcaagtt tgggccaacg
     1501 ccgattatcc caaggatgcc cgccgcgccc gctcatacgg tgcagtcggc attgggctgt
     1561 gccgcactga gcatatgttc tttgaaccag aacgcctgcc gattgtgcag cgcatgatcc
     1621 tcgctgaaac cagtgagacg cgcaaagctg ccctggacga acttctgccg tcccaacgca
     1681 ccgacttcga gggtcttttt gaggccatgg atggctaccc ggtcatcatc cgcctgattg
     1741 accccccact gcatgaattt ttaccctcgg aagaagaatt actcgaagag gtcatcacca
     1801 tgcgagttaa gggtgaaaac aagggactgg cagagaaaga gaaattgctc gccagtgtca
     1861 aatccatgca cgaaagtaac cccatgatgg gtctgcgcgg tgtgcgcttg agcatccaga
     1921 tgccagagat tgtcgaaatg caagtgcgtg ccattttcga ggcagcagcc aacatgacta
     1981 tcaagggagt ggtagttaaa ccggaagtga tgatcccatt gactggaacg atcaatgagc
     2041 tgcgctggat ccaaccccgc ctggagaaga tcgccaaggc tgttatggat gagaaacaaa
     2101 tcaccttttc ctataagttc ggcaccatga tcgaaatccc ccgtgccgcg gttacagcta
     2161 ctgaaatcgc cgagttagct gagttcttct cctttggcac caacgactta acccaaatga
     2221 cctttggcta tagccgagat gacgccgaac gcaacttcct tgtgacttat gtcgaacagg
     2281 gaatcttgtc caataacccc ttccagaccc tggatcgagg tggagtagga cgattgatgg
     2341 atttggctgt caaagacgga cgccagaccc gacctgactt agaggtgggt atctgcggcg
     2401 aacatggcgg cgatccaagc tctattgagt tttgtcacac gattggcaat aactatgtga
     2461 gctgctctcc cttccgggtg ccgattgctc gattagcagc tgcccattcc gcactgaagc
     2521 ataaaggtgt gtctattgcc gaggataagt aggtagcacc aatcctcttt cctccgagcg
     2581 agaaaatcct ttaaagttaa tgggatcgcg ctggctaact gccgcttgcg cgatcccatt
     2641 atttatttaa tgcgttacgt agtcgcagga atggaaacgg ttaccttgtt gccaggatat
     2701 cgcccaacaa gctctccctc agcaagatgt acacggtttt gaccccctca aactgccgga
     2761 tggtggtcca aatttgagcg cgcacacgcc catcgtcaca ccgatcacct gagcgaacat
     2821 aggtgccatc cag
//
LOCUS       DBNHOH010000087         2090 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1670805, whole genome shotgun sequence.
ACCESSION   DBNHOH010000087 DBNHOH010000000
VERSION     DBNHOH010000087.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1670805"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="AC16V7_01530"
     CDS             <1..724
                     /locus_tag="AC16V7_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255826.1"
                     /translation="VDGAAPFLMASATTEDPTVCGVPAPTLNTATPGHTQVTLEWSAA
                     TGATGYNVYYDQAGKSQFVANAGNNITYLDTGLTNGVEYCYKVTAYDATCESGFSNIL
                     CAVPNNQGQAQVGVSKIEIGRYVTTGKGKDTVTTFELTDQFNPGDGVVIRAYVVDAST
                     GLPVANATVDLLISGPESVPLTSQPSDTTGMVEVSWQTVAPNKRGQGGTTTGTYTVTV
                     TNVTATGYTWDGVTTSTTFTIQ"
     gene            874..1290
                     /locus_tag="AC16V7_01535"
     CDS             874..1290
                     /locus_tag="AC16V7_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR6255827.1"
                     /translation="MSLWQHVLRLLGYQKTSDRLSFSVDVSLIRSLQDLAEQESRSET
                     ELAAELLSYALAQRDVAEANLQRWRGLSEREQQVTALICLGFTNRQIAARLVISPETV
                     KSHVSRVLLKFGLRTRTELRRTLADWDFSAWRDIQF"
     gene            1377..1940
                     /locus_tag="AC16V7_01540"
     CDS             1377..1940
                     /locus_tag="AC16V7_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase family protein"
                     /protein_id="HIR6255828.1"
                     /translation="MDLIPEPLSGKITLAIVPRRAIRPLLGLAARLAQRAPLLVLDGG
                     NCFNAYVVALALRQQTTDVTAALERIRVSRAFTCYQMVTLLEETPATPVATLVLDFLA
                     TFRDENVPQGERLRLIRICLERLRFLASAAPLLVSASPDSGPPMSGGRGRRQLQPDDR
                     LLALLEEAADQVWRFGPPPPTVQLRLL"
BASE COUNT          401 a          641 c          624 g          424 t
ORIGIN      
        1 cgtggacggc gcggcgccgt tcctgatggc ctcggctacc accgaagacc caactgtctg
       61 cggcgtgccg gcaccaaccc ttaacacagc cacgcccggc cacacgcagg tgaccctaga
      121 gtggtcggca gcgacgggcg ccaccgggta caacgtctac tatgaccagg ccggcaaatc
      181 ccagttcgtt gctaatgctg gaaacaacat cacctacctc gacacgggcc tgaccaacgg
      241 ggtggagtat tgctacaagg tcaccgccta cgacgccacc tgcgaatcag gcttcagcaa
      301 cattctgtgt gctgtaccca acaaccaggg gcaggcccag gttggggtga gcaagatcga
      361 gatcggtcgt tacgtgacca cgggcaaggg caaggacacc gtcaccacct ttgagttgac
      421 cgatcagttc aacccaggcg acggcgtggt gatccgcgcc tacgtcgtgg acgccagcac
      481 cggcctaccg gtggccaatg ccactgtgga cctgctgatc agcgggccag agagcgtgcc
      541 cctgaccagt caaccgagcg acactactgg catggtcgag gtctcctggc agactgtggc
      601 accaaacaag cgcggccagg gtggcaccac gaccggtacc tataccgtca cggtcacgaa
      661 tgtgaccgcc accggctata cctgggatgg ggtgacgacc agcaccacgt tcaccatcca
      721 ataagctgct tcttcgccaa actgagttag atcatccccc caagcttgcc accgctgctt
      781 ggggggatga tatcttaaaa gggcttgtca tcagaacaaa tgttctgtat aatggagaag
      841 gagtcgatta tcaggatgat tttctaggat gtcatgtctc tctggcaaca tgtattgcgc
      901 ctgctgggtt atcaaaaaac ttccgaccgg ttgtctttct cggtggatgt aagcttgatt
      961 cgctcgctgc aagatctggc tgagcaggag agccgctctg agacggagct ggcggcagag
     1021 ctgctctctt atgccctggc tcagcgcgat gtggcggagg ccaacctgca gcgctggcgc
     1081 ggcctctcag agcgggagca acaagtgact gcgctgatct gcctgggctt cacgaaccgg
     1141 cagatcgccg cacgcctggt aatatcgccg gagacggtga aatcccatgt cagccgggtg
     1201 ttgctcaagt ttgggctgcg cacccggact gagctgcgcc gcacgttggc ggattgggat
     1261 ttcagcgcct ggcgagacat ccagttctaa tccccctatg ggttaacccg tttttccccc
     1321 ttttatcccc cgcgcatggg attgcgccgg cttgccgtct gcgctactct ggaagtatgg
     1381 acctgatccc agagccgctt agcggtaaga tcacactggc gatagtgcca cgccgggcca
     1441 tccggcccct gctggggctg gcggcgcgcc tggcgcaacg cgcccccctg cttgtgctgg
     1501 acgggggcaa ctgcttcaac gcttacgtag ttgctctggc gctgcgccag cagactaccg
     1561 atgtgaccgc tgccttggag cgcatccgcg tttcgcgggc gttcacttgc taccagatgg
     1621 tgaccttgct ggaagagacc ccagcaacgc cagtggcgac cctggtgctg gatttcctgg
     1681 cgacttttcg cgatgagaac gtgcctcaag gggagcggct tcgcctgatc agaatttgtc
     1741 tggagcgttt gcgcttcctg gcttcagctg caccgttgct ggtgagcgcc tcgcccgata
     1801 gcggtccgcc gatgagcggg gggcgcggtc gacgccagct tcagcctgat gatcgactgc
     1861 ttgccttgct ggaggaagcc gccgatcaag tgtggcgctt tggccccccg ccgccaactg
     1921 tgcagttgag attgctctag caattatccc tatgcaaacc gccccctttg ggttatacta
     1981 ttgacaggag ccacgcagat gcgtggctta ctttccgctc ctgaccggct cccagccgag
     2041 tttaggggca aacccgtgga aaggaggcat cccctgcatg cgcgagtatg
//
LOCUS       DBNHOH010000088         4056 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_388440, whole genome shotgun sequence.
ACCESSION   DBNHOH010000088 DBNHOH010000000
VERSION     DBNHOH010000088.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4056)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_388440"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="AC16V7_01545"
     CDS             <1..506
                     /locus_tag="AC16V7_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HIR6255829.1"
                     /translation="DIDIDLYPNAESLMETMRGRGLQPDPKATRGKLIESLMGDYLEP
                     NLIQPTFIYDYPRDVSPLAKSLPGNPNLVERFEGFVAGFELCNAFTELNDPLDQEARF
                     REMGRDYAADDEERHPMDDDYLRAMRYGMPPNGGFGMGIDRLTMLFTDSANIREVILF
                     PHLRERD"
     gene            859..1290
                     /locus_tag="AC16V7_01550"
     CDS             859..1290
                     /locus_tag="AC16V7_01550"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6255830.1"
                     /translation="MIKVKGSILINAPVEKVFAFMDDPCNLPEIWPSMIETHAKGPSS
                     AGGQDFEWEYKMAGLRIKGESEVVERIPNRKMITKSKKGIETTFVWDYVSEAGGTRLT
                     VEAEYNIPVPLVGRLAESVIVKQNEYEANMLLENLKTRMEG"
     gene            complement(1475..3022)
                     /locus_tag="AC16V7_01555"
     CDS             complement(1475..3022)
                     /locus_tag="AC16V7_01555"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR6255831.1"
                     /translation="MSNDQAASKPSLWHRASLLIYPFLLVYYPILALRAHNIVYVNLP
                     SILRSLLLVTAGTAILIAITHLFVRNLAKTSIIVSLIGMFFLSYGHLYIKIEETLGSP
                     VRHRYLVAASFLILLLVTILILKKDQVARVLAQFLATTSIVLVAIVVFQTVNYDIGVY
                     RAEAASAQEEAPVAPVQADEKLPDLYLIILDAHTRTDVLEARFGFDNSNFIQKLNDMG
                     FYVASCSQSNYASTKLSLTSTFYGDYIQNFVEEGEILPSLKRSALNQTLKSLGYKIFA
                     FENRASGHFDLKEDVLLSRNQMAFGKFDFIGGISEYEKMLIDTSFLRFVVDTELIPGF
                     DNTRTQEWELWEHYYQTNYILAELEKVPDSPGPKFVFVHIMVPHSPFIFAPDGSYQHN
                     NSPIDGYRANTEFIDSHLPPVLQTILEKSNPTPIIIVMGDHGPSTRKTIAKEMRMATL
                     NAYLVNEAAKAQMYPAMTPINATRIILNAHYNGAYALLEDVSYYAYKGSQLPDAEIIT
                     NNCPPTP"
     gene            complement(3170..4039)
                     /locus_tag="AC16V7_01560"
     CDS             complement(3170..4039)
                     /locus_tag="AC16V7_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIR6255832.1"
                     /translation="MSNSPRKVKQVIEPQYVIEGAGVLLRRSFGPNRTNLFDPFLLFD
                     HFAFNDPIEGPIRGFPTHPHRGIETVTYILEGNVRHRDSLGNTGIIGPGDVQWMTSGR
                     GILHEEMPRRGPNGVIYGFQLWVNLPAAQKMSQPRYQEVNADSIPTVEKQGAKVRLVA
                     GEFGGVKGPVTQIAAQPLYMDVTLDPGAEFYLPVPVGHTAIAYLFEGEGLFGLDAEGQ
                     GEFTPAVRMIEFEDGDHVRVQAGQGSHARFMLMAGAPFKEPIFPYGPFVMNTQEEIQQ
                     ALNDLRNGTFVQN"
BASE COUNT          990 a          957 c         1161 g          948 t
ORIGIN      
        1 gcgatatcga tatcgacctg taccccaacg ctgaaagcct gatggaaacc atgcgtgggc
       61 gcggcctgca gcctgatccg aaggccacgc gaggcaagtt gatcgaatcg ctgatgggcg
      121 attaccttga gcccaacctg atccagccga cctttatcta tgattacccc cgtgatgtct
      181 cacctctggc gaaaagcctg ccgggtaacc cgaatttggt tgagcgtttc gagggctttg
      241 tggccggatt tgagctgtgc aatgccttta ccgagcttaa cgacccgctg gatcaggaag
      301 cccgcttcag agaaatgggc cgcgattacg cagccgatga cgaagagcgc caccccatgg
      361 atgatgacta cctacgcgcc atgcgctatg gcatgccacc taacggtggt tttggcatgg
      421 gcatcgaccg cctgactatg ctgttcaccg atagcgccaa tatacgcgag gtgatccttt
      481 tcccgcacct gcgggagagg gactagagcg aaaatttttt caagtgctac ttgacagccg
      541 tgcccaattt tgctattctg tttacaggta agagtggccc tggctggatt gtggtcacaa
      601 cgagcaaagg tatatcagct ccagcctgac gggtgccgga gcgatattgg gggacatggg
      661 cacctgaggg cggggcatat cgaatttccc tccatagctt agccatttcg aagtcagcca
      721 gcttagctcc gtgagctcgg ggtccggtaa attttttcat atggacctat cgggttttca
      781 ctgatgtcaa gcaaacatcc actggatggt ttgtccatcg tattcgtatc caactaataa
      841 aaagcaggag gaatgacaat gatcaaagtg aagggtagca ttctgatcaa tgcaccggtt
      901 gaaaaagttt tcgctttcat ggatgaccca tgcaacctgc cagaaatatg gccgagcatg
      961 atagaaacac atgcaaaagg accttcaagc gctgggggtc aggatttcga atgggagtac
     1021 aagatggctg gcttgcgcat taagggtgaa tcggaggtcg tggagcgcat acccaatcgt
     1081 aaaatgatca ccaagagcaa gaaaggcatc gagacaacat tcgtgtggga ctatgtatct
     1141 gaagcaggcg gcacccgcct gaccgtagag gctgagtaca atatcccggt cccattggtg
     1201 ggtaggttgg ctgagagcgt gatcgtcaag caaaatgagt acgaggccaa tatgctattg
     1261 gagaatttga aaacccgaat ggagggctga gagccggcaa ataccaatct ggaagaccat
     1321 ctgatgtgga ggccgcgccc cgctgtgccg actgatcttc cctccggtaa aatttaggtg
     1381 ggtggctcgg tggaaagtat gccagcgggg cgcaggtggt gattagatca agcgccctgg
     1441 gttggtttgt caaatgtata tagggtaaag ggggctatgg cgtaggtggg caattattgg
     1501 taatgatttc tgcatcaggc agctgggagc ctttgtaagc ataataactg acgtcttcaa
     1561 gaagcgcata agcaccgttg tagtgggcgt ttaggataat gcgcgtcgca ttgatcgggg
     1621 tcatggcggg atacatttgc gctttggctg cctcattgac caggtaggca ttcagggttg
     1681 ccatgcgcat ttccttggcg atcgtttttc gggtggatgg cccgtgatcg cccatgacga
     1741 tgatgatggg ggtcggattt gatttttcga gtatagtttg caggacaggc ggcaggtgac
     1801 tatcgatgaa ctcggtgttg gcacgatagc catcaatcgg gctgttattg tgctggtagg
     1861 agccatctgg agcaaatata aacggcgagt ggggtaccat gatatggaca aagacgaatt
     1921 ttggcccagg agaatcggga actttctcca gttcggcgag aatgtaattg gtctggtaat
     1981 agtgctccca caactcccat tcctgtgtgc gggtgttgtc gaagccaggg atcagttctg
     2041 tgtcaaccac aaagcgtaaa aatgacgtgt caatcagcat tttctcatat tcgctgatgc
     2101 caccgataaa gtcaaatttg ccaaaggcca tctggttgcg cgaaagcagg acatcctcct
     2161 tgaggtcgaa atggccgctg gcgcgatttt caaaggcgaa gattttatag cccaaagatt
     2221 tgagcgtctg gttgagggca cttcttttca aagagggtaa gatttcccct tcctcgacga
     2281 agttctgtat gtaatcgcca taaaaggtgg aggtcagcga gagtttagtg gaggcgtaat
     2341 tgctctggct acaactggcc acgtaaaacc ccatatcatt caacttttgg ataaagtttg
     2401 agttgtcaaa tccaaagcgc gcttcaagca cgtccgtgcg tgtgtgagca tccaagataa
     2461 tcaggtataa gtctggcagt ttctcgtcag cctgtacagg cgcaacggga gcttcttctt
     2521 gggcagacgc ggcttcggcc cgatacacgc caatgtcgta gttaacagtt tggaatacta
     2581 ctattgcgac caggacaata ctggttgtgg caaggaattg cgccagcacc ctggcgacct
     2641 ggtctttctt caagatcagg atggtaacca gcagcaagat caagaacgag gcggcgacaa
     2701 gatagcgatg ccggaccggg ctgcccaagg tttcttcgat cttgatatag aggtgaccgt
     2761 aggacaggaa gaacatcccg attaaagaca cgatgatgct ggttttagcc aaattgcgga
     2821 cgaaaaggtg ggtaatggca atgaggatcg ctgtaccagc tgtcaccagg aggaggctgc
     2881 gcaggataga cggcaggttg acgtagacaa tgttgtgggc gcgcagagcc agaatcgggt
     2941 aatagaccag taaaaacggg taaattagca ggcttgctct gtgccagagg gatggcttgc
     3001 tggcggcttg atcattggac ataaaaaaag ccttccattg tcaggcagtc aagaggcttg
     3061 gactttaata ccgccagagt atatcataat ggacgggcga aaaagtcgcc agcccgatgc
     3121 agggctaaac atccccacct aattttgagc agcctatcag tacgctgtgc tagttctgga
     3181 cgaaggtgcc gttgcgcaga tcgttcaacg cttgctggat ctcttcctgg gtgttcatca
     3241 caaatggacc ataagggaag atcggctctt tgaacggcgc gccagccatc agcatgaagc
     3301 gggcatgaga gccttgtcca gcttgaacgc ggacgtggtc gccatcttca aattcgatca
     3361 tgcgcaccgc gggggtaaat tcaccctgcc cttcagcgtc cagaccgaat aacccctccc
     3421 cctcgaaaag ataggcgatg gcggtatggc ctactgggac aggcaagtag aattccgcgc
     3481 caggatcgag agtcacgtcc atatagagcg gttgggcggc aatctgtgtc acaggtcctt
     3541 taacaccccc aaattcaccc gccaccaggc gtacttttgc accctgtttt tctacggttg
     3601 ggatgctgtc ggcattcact tcctgatagc gcggctggct catcttctgg gcggcgggca
     3661 ggttgaccca tagctggaag ccatagatga cgccattcgg cccgcgcctg ggcatctctt
     3721 cgtgtaagat tccgcgcccg ctggtcatcc actgtacatc gccgggacca atgatgccgg
     3781 tattgcccag gctgtcgcgg tgtcgcacat tgccttccag tatgtatgta acggtttcaa
     3841 tgccgcggtg tgggtgggtg ggaaagccac ggatagggcc ttcgatcgga tcgttaaaag
     3901 caaaatggtc aaacagaaga aacgggtcga aaaggttggt gcggttgggt ccaaaggagc
     3961 gccgtaacag cacgcctgcg ccttcgataa cgtactgcgg ctcgataacc tgtttaactt
     4021 tgcgagggga atttgacata aaaccaactt tctatc
//
LOCUS       DBNHOH010000089         1043 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_901642, whole genome shotgun sequence.
ACCESSION   DBNHOH010000089 DBNHOH010000000
VERSION     DBNHOH010000089.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_901642"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="AC16V7_01565"
     CDS             <1..757
                     /locus_tag="AC16V7_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012288364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HIR6255833.1"
                     /translation="LNDVIWIKTNPMPNFRGVRFTNAHETLIWTCKSKGAKYTFNHHA
                     MKALNDELQMRSDWLLPICTGSERIKVDGKKAHSAQKPEALLYRVLLAATNPGDIVLD
                     PFFGSGTTGAVAKKLSRRWIGIERDPEYIQVAQERIDAIPDAQLDPAVLDVRDRRRRE
                     PRVPFGSLLEAGLLHPGQALYFRKQRSKAAVVKPDGKLKLGDFEGSIHAVGSHLMGGS
                     PCNGWDNWYYETETGELLVIDELRKKVRASLAG"
     gene            complement(754..>1043)
                     /locus_tag="AC16V7_01570"
     CDS             complement(754..>1043)
                     /locus_tag="AC16V7_01570"
                     /inference="COORDINATES: protein motif:HMM:NF048429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF7670 domain-containing protein"
                     /protein_id="HIR6255834.1"
                     /translation="QIAIFMLFGLGEMASGEFSGAGHLLPAVATLVLMLLSWKRPIEG
                     GAALFVIGAVTTTDFSDTTALLIMAAPQIVSGLMFLLAGWIAQGGASPEGN"
BASE COUNT          244 a          285 c          322 g          192 t
ORIGIN      
        1 cctcaatgat gtcatctgga tcaagaccaa cccgatgcct aattttcggg gtgtgcgctt
       61 caccaacgcc cacgagacat taatttggac gtgtaaatcg aagggggcaa aatatacctt
      121 caaccatcac gccatgaaag ccctcaacga tgaattgcag atgcgcagcg actggctatt
      181 gcctatctgc accggttcag agcgcatcaa ggtggatggc aagaaagccc actcggcgca
      241 gaaaccggaa gcgctcctct accgagtgct actggcggcg accaatccgg gagatatcgt
      301 gctggaccca tttttcggca gcggcaccac tggggcggtg gcgaagaagc tgagccgtcg
      361 ctggatcggc atcgagcggg acccagaata tattcaggtg gcacaagagc gcatcgatgc
      421 tattccagat gcgcagcttg accctgccgt gctggatgtg cgcgaccgca ggcgccggga
      481 gccgcgcgtc ccatttggat cgctgctgga ggcgggcctg cttcacccag ggcaggcatt
      541 atatttccgc aagcaaaggt cgaaggcggc ggtggtgaag ccggatggta agttaaagct
      601 aggggacttc gaaggctcga tccacgctgt gggtagccac ctgatgggcg gcagcccatg
      661 caatggctgg gacaactggt attatgagac cgagacgggg gaattgctgg tcatcgatga
      721 gctgaggaag aaggtcaggg caagtctggc aggttaattg ccttcgggtg acgctcctcc
      781 ctgtgcgatc catccggcga gaaggaacat caggccggag acgatctgcg gggctgccat
      841 gatcagtagc gctgtagtgt cagagaaatc tgtagtagtc actgcgccga tcacaaacag
      901 ggctgcgccg ccttcgatag gccgcttcca ggaaagcagc atcagcacca gggttgcaac
      961 cgctggcaga aggtgcccgg ccccgctgaa ctcgccggac gccatctcgc ctagaccaaa
     1021 caacatgaag atggcaatct gca
//
LOCUS       DBNHOH010000090         2216 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_68253, whole genome shotgun sequence.
ACCESSION   DBNHOH010000090 DBNHOH010000000
VERSION     DBNHOH010000090.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_68253"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..518)
                     /locus_tag="AC16V7_01575"
     CDS             complement(150..518)
                     /locus_tag="AC16V7_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020404554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR6255835.1"
                     /translation="MTTIRRFEDIQAWQEARSLVKQIYALTEVGAFSKDYGLRDQLRR
                     ASVSVMANIAEGFDCDSNLEFARFLGIARRSAVEVQSLLYAALDVRYINQETFQACYE
                     QANKVKAFVSAFKSSLKKRS"
     gene            complement(588..1655)
                     /locus_tag="AC16V7_01580"
     CDS             complement(588..1655)
                     /locus_tag="AC16V7_01580"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0070085 - obsolete glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HIR6255836.1"
                     /translation="MEIHIGSHTIGLKHPTYFIADIAANHDGDLERAKLLIRLAKEAG
                     ANAAKFQNFRAPQIVSDYGFRSMGGQVSHQANWKKSVFQVYQDASVPFEWTPILKEDC
                     DQVGIGYFSSPYDFEAIDMLDAYVPAYKIGSGEIDWLEALDRMARKGKPVLLATGACT
                     IGEVQQAVHTVLASNPQLVLMQCNTNYTASPENFDHIHLNVLKTYAAMFPQVILGLSD
                     HTHGPATVLGAVTLGARVIERHFTDDNNRVGPDHKFAMNPSGWADMVARTRELERALG
                     SADKTVAGNEQDTTVVQRRCLRAARDIQAGEVFTREMIDVLRPATPGAILPPHIPEVI
                     GTRAIHDIPKGKELRWVDLGK"
     gene            complement(1746..>2216)
                     /locus_tag="AC16V7_01585"
     CDS             complement(1746..>2216)
                     /locus_tag="AC16V7_01585"
                     /inference="COORDINATES: protein motif:HMM:NF015146.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HIR6255837.1"
                     /translation="KLWVKDGVQGLLRKGGYADVTPFWEAVMHRATELFERPVKPGVD
                     YALTEVVHCKSRRQKGVDEALEECSQRYLKRVLEISGANLIVVLGATARKVVKDMFSI
                     SPRTHLSDPIVIKNMARIFVFLPHPNAYLPNTFSENFSAEELKQIQAHLFKASQ"
BASE COUNT          520 a          545 c          602 g          549 t
ORIGIN      
        1 tcgctccaat tgaaggtaat agatctggtg ctccgtctgt gcaagctttg agaggaagcg
       61 atgatcgtgg gttgtatagt cgcgggtgaa gtaaatgata cgcataaaga aggtgcgatg
      121 tgcgacgtgc gatgtacggt gtgtggaaac taagaccgtt tttttaatga actcttgaaa
      181 gcactgacga atgccttgac cttattggct tgttcatagc aggcttggaa agtttcttgg
      241 ttgatatacc taacgtcaag tgcagcataa agcaacgatt ggacttcaac cgctgaacgc
      301 ctggcgatac cgagaaaacg tgcaaactca aggttcgaat cgcaatcgaa gccttcagca
      361 atattggcca taactgaaac actcgctcga cgaagttgat ccctcaaacc ataatctttc
      421 gaaaaagccc cgacctcggt caaggcatat atctgcttta caaggctacg agcttcctgc
      481 caagcttgaa tgtcctcaaa acgtctgatt gtggtcatgt aatacttcct gcattttcct
      541 gtttcgcatt acatcgcact tagcacgtcg cacttagcac gccatattta cttaccaaga
      601 tctacccacc tcaactcctt gcccttaggg atatcgtgga tggcgcgcgt gccgatcact
      661 tcggggatat gcggcggcag gatagcccca ggtgtggcgg ggcggagaac atcgatcatc
      721 tcgcgggtga agacctcacc agcctggata tctcgggcgg cgcgcaggca gcggcgctgc
      781 accacggtgg tgtcttgctc gttgccggcg acggttttat cggcagaacc cagggcgcgc
      841 tccagctcac gcgtgcgggc taccatgtct gcccatccag agggattcat ggcgaattta
      901 tggtccggcc caacgcggtt gttatcatcg gtgaaatggc gttcgatcac gcgtgcccct
      961 aaagtaactg cgcccaacac ggtggcaggt ccgtgagtgt ggtcagacag tcccaggatc
     1021 acctggggga acatcgccgc gtaggtttta agaacgttta agtggatgtg atcgaagttt
     1081 tccggactgg cggtgtagtt ggtgttgcac tgcatcaaaa cgagttgagg gttcgatgcc
     1141 aggacggtgt ggactgcctg ctgtacttcg ccgatggtgc aggctcctgt tgccagcagc
     1201 accggctttc ccttgcgcgc catgcgatcc agcgcttcca gccaatcgat ttctccggaa
     1261 ccgatcttgt aagcgggtac ataggcgtcc agcatatcga tcgcctcgaa gtcataaggc
     1321 gaagagaagt agccaatgcc aacttgatca cagtcttctt tgaggatcgg cgtccactcg
     1381 aaggggaccg aggcgtcctg gtagacctgg aagactgatt ttttccagtt cgcctggtga
     1441 gagacctgcc cgcccataga gcggaaaccg tagtcggaga caatctgggg agcgcggaag
     1501 ttctggaact tggccgcgtt tgccccggcc tcctttgcca agcggatgag cagcttggcg
     1561 cgttccaggt cgccgtcatg gttggcggcg atatcagcga tgaaataggt ggggtgcttc
     1621 aggccaatgg tgtgggaacc aatgtggatt tccatagaga ctccgagatt ctagagtaga
     1681 gaggatgatc gtataagcca gtctattatg acaaacagga agacaatgga agcgtggcaa
     1741 tctcctcatt ggctggcttt gaataaatgt gcttgtattt gtttcaattc ttcggcagag
     1801 aaattttccg aaaaagtatt gggtaggtat gcgtttgggt ggggaagaaa gacaaaaata
     1861 cgggccatat tcttaatcac tatcggatca gataagtgtg tgcggggaga aatggaaaac
     1921 atgtctttta ccacttttct cgctgttgcg ccgaggacga cgatgaggtt tgcaccagat
     1981 atctcgagaa ctcttttcaa atagcgctgg ctgcattcct caagcgcctc atccactcct
     2041 ttttgtcggc gcgacttgca gtgtacgact tctgtcagtg cgtaatcaac cccaggtttt
     2101 acaggtcgtt caaagagttc agtagcccta tgcattaccg cttcccaaaa aggagtgaca
     2161 tctgcatagc cgcccttacg taataaccct tgcacgccat cttttaccca taactt
//
LOCUS       DBNHOH010000091         3831 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_324581, whole genome shotgun sequence.
ACCESSION   DBNHOH010000091 DBNHOH010000000
VERSION     DBNHOH010000091.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_324581"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2247
                     /locus_tag="AC16V7_01590"
     CDS             <1..2247
                     /locus_tag="AC16V7_01590"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR6255838.1"
                     /translation="VPAARIETGDRARFAGDWERALLEYETALGNSQDAEIQSAALLG
                     IGRTRTSIGDPAGAIDALNRLIQNYPQSAHVPYAHFALAEAHSALGQHSQAADEYLNY
                     LVIRPGLVDAYVLDIRGDALQAAGRFGEALVDYRGALQAPSFLDSLTIEIKIARAHAA
                     VGDYETALGLYQDIYTRSDNDFVKAQMDLFMGQAYIAQGNLEQAYTVFQDAVNNYPTS
                     LDSYQALLALVEADIPVDERNRGIVDYYAGQYGVAIAAFDRYFQKGGDDPATARYYNG
                     LSLRALGGYQDAVSQWDIIIQNYPDNRFWDDAWEQKAYTQWAFLEQYPEAIQTLLGFV
                     ASAPNHSRAGEFLLDAALVAERSGDLAQAVQYFERVAAEYPGYEKATRSLFLAGVTRA
                     RLGDYDNASKVFQRLLENATTPEERASAFLWLGKCQLALGDPSAAAMSWELAANSDPT
                     GYYSERAHDLLRQRIPFQPPEQYDLSMDLNAERVEAENWLRGTFNVPQEIDLSTPAAL
                     LGDPRLVRGSELWELGMYDQARDELEAVRQSLQADAVGSYLLANYLLELGMYRSAILS
                     ARQVLDLAGMSDVETISAPVYFNHIRFGPYFAELVLPAAQEKNFHPLFLYSVIRQESA
                     FEGYVRSTAGARGLMQIIPSTGEEIANDLGWPPNYSAEDLYRPLVSVRLGTDYLAKWR
                     DHLDGDLYAALAAYNGGPGNAIEWQKKAPNDPDLFLEIVPFEETRNYIRGIYEIFGLY
                     RRIYERIP"
     gene            2274..3350
                     /locus_tag="AC16V7_01595"
     CDS             2274..3350
                     /locus_tag="AC16V7_01595"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6255839.1"
                     /translation="MSVHPFITPSFTSSIKLPWQYTAIDHPIISSLTTALEARAGLID
                     TQHEGALRLFNGFYEGDPHLVVDLYARTLVIFNHAEPHETGQAAVQTAQEFFLSHLPW
                     VEAIVTKTRYAHTLDARHGRLVYGEKPDRRVREHGTWYAIDLMIAQDASLYLDTRLLR
                     LWAMQNLARKSVLNTFAYTGSLGVAARAGCATTVVHLDLSRKFLNIAKTSYTLNGFPI
                     HKADFQAGDFWTQTSRARRSGESYDCVILDPPFFSVTPKGRVDLAGDSARLINKVRPL
                     VNRGGYLVALNNAIFISGADYLQTLEELCADGYLAIEELIPVPGDVTGYPQTIRRSPP
                     VDPAPFNHSTKIAILRILNRSSHE"
     gene            3343..>3831
                     /locus_tag="AC16V7_01600"
     CDS             3343..>3831
                     /locus_tag="AC16V7_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR6255840.1"
                     /translation="MSKEIIFENARQVAFQDLPDPPLTARQVRVRTLYSGISAGTEMT
                     AYRGSNVYLHKHWDAKNRLFISAETPSSTYPLGGFGYEEVGEVIEIGEQVTCLKQGDI
                     VFGTWGHRTQHILEEEYASQRIKPAELEPILGIFSHIGPIALNGILDAQIHIGENVAV
                     FGL"
BASE COUNT          868 a         1120 c         1007 g          836 t
ORIGIN      
        1 gtaccagcag cccgcataga aaccggcgac cgggcacgct ttgcgggtga ttgggagcgc
       61 gccctgctgg agtacgagac agccctaggg aacagccagg atgcggaaat ccagagcgcg
      121 gcgctgctag gcattggacg aacccgcacc tctataggcg accccgctgg cgccatcgac
      181 gcactcaatc gcttgataca gaattatcct caatcagcac atgtcccata cgctcacttc
      241 gcccttgctg aagctcattc agccctaggg cagcatagcc aggccgcaga tgaatacttg
      301 aattacctgg tgatccgccc aggtctggtg gatgcgtacg tcctggacat acgcggcgat
      361 gccctgcaag ctgctgggcg ctttggtgag gcgctggtag actaccgggg cgccctgcaa
      421 gcacccagct tcctagacag cctcactatt gagattaaga tcgcccgggc gcacgctgcg
      481 gttggggatt acgagactgc tctgggctta taccaggata tttacacccg atcagataac
      541 gattttgtca aagcacagat ggatctgttc atggggcagg cgtatattgc ccagggcaat
      601 ctcgagcagg catacacggt cttccaggac gcggtgaaca actaccctac atccctggat
      661 tcttatcaag cgcttttggc tctagtagaa gcagacatcc cagtcgatga acgaaaccgc
      721 ggtattgtgg attattacgc tggccaatat ggtgtcgcca tcgccgcctt tgatcgctat
      781 ttccagaaag gcggcgatga ccccgccaca gcgcgctact ataacgggct aagcctgcgt
      841 gccttgggag gataccagga tgccgtttcc caatgggaca tcatcataca gaattaccca
      901 gacaaccgct tttgggatga tgcctgggag cagaaagcct atacgcagtg ggcattcctg
      961 gagcagtatc cagaggctat tcaaaccttg ctcggctttg tagccagcgc tcccaatcat
     1021 tctcgtgctg gtgagttcct gctcgatgcc gctctggtcg ccgagcgttc aggcgacctg
     1081 gcccaggcgg ttcaatattt tgagcgcgta gctgccgaat accctggcta tgaaaaggca
     1141 actcgatctc tcttcctggc tggcgtcacg cgggcacgcc tgggagatta tgacaacgca
     1201 tccaaagtct tccagcgcct tttagagaac gccactacac ctgaggagcg cgcttcggcc
     1261 ttcctctggc tgggcaaatg ccagctcgcc cttggcgatc cctccgctgc cgcaatgtct
     1321 tgggaactgg ctgccaattc tgaccctacc ggttactaca gcgagcgcgc ccacgacttg
     1381 ctgcgccagc gcatcccatt ccaaccccca gaacaatacg acttgtccat ggacctaaac
     1441 gcggagcggg tcgaagctga gaactggctg cgaggtacat ttaatgtccc tcaagagatt
     1501 gacctatcca ccccggcggc tctcctcggc gacccgcgct tggtgcgtgg tagcgagctc
     1561 tgggaattgg gtatgtacga ccaagctcga gatgaactcg aagccgtacg ccaatcactc
     1621 caggcagacg cggtaggcag ctacctgctg gctaattacc tgctcgagtt gggcatgtac
     1681 cgctcagcaa tcctatcagc caggcaagtg ctggacctgg ctggcatgag cgatgtcgag
     1741 acgataagcg cccccgttta cttcaaccat atccgctttg gaccgtactt tgcagagttg
     1801 gtcttaccag ccgctcaaga gaaaaatttc caccccttgt tcttatatag cgtgattcga
     1861 caggaaagtg ctttcgaagg ttatgtgcgc tccaccgccg gggcgcgcgg actgatgcag
     1921 atcattcctt ccaccgggga ggaaatcgcc aatgaccttg gctggccgcc aaattacagc
     1981 gctgaggact tgtaccggcc cctggtcagc gttcggctcg gtacggacta cctggcaaaa
     2041 tggcgcgacc acctggacgg tgatctttac gctgcgctgg cagcctacaa cggtggaccg
     2101 ggcaacgcca tagaatggca gaaaaaggcg cccaatgacc cagacctatt tttagaaatc
     2161 gtccccttcg aggagacgcg caattatatc cgcgggatct atgaaatctt cggcctgtat
     2221 cggcgcatct acgagagaat cccataagat agaagccttg gcagtgtgtt ttggtgagcg
     2281 tccatccatt tatcacccca tcatttacat cctccatcaa gctaccctgg cagtataccg
     2341 caatagatca cccaatcatt tcttccttga ccacagctct tgaagcccgc gctgggctga
     2401 tcgacaccca gcacgagggt gcattgcgct tatttaacgg attctacgaa ggcgacccac
     2461 acctcgtagt ggacctatat gctcgcacac tggtaatttt caaccacgcc gagccccatg
     2521 agacaggaca ggcagcggtc caaaccgccc aggaattctt tctctcccac cttccctggg
     2581 ttgaagcgat tgtcacaaaa acccgttatg cacacactct ggatgccagg cacgggaggc
     2641 tggtctacgg cgaaaagcct gaccgccgcg tgcgagaaca cggcacctgg tatgcgatag
     2701 acttgatgat cgcacaggat gccagtcttt acctggacac gcgcctgctg cgcctgtggg
     2761 caatgcaaaa cctggcaagg aaaagtgtgt taaatacttt cgcatacact ggcagcctcg
     2821 gggtagccgc acgcgctggc tgcgcgacta cagtcgtgca tctggacctc agccgtaaat
     2881 ttctcaacat tgccaagaca tcctatacgt taaacggttt ccccatccac aaggcagatt
     2941 tccaggctgg tgatttctgg acgcaaacca gccgtgctcg ccgttcgggg gagagttacg
     3001 actgcgtcat tcttgacccc cctttcttct cggtgacacc caaagggcga gtggacttag
     3061 ctggcgacag cgcgcggctg atcaacaaag ttcgtcccct ggtcaaccga ggtggttatc
     3121 tggtggcgct caacaatgcc atatttatca gtggggccga ctacttgcaa actttggaag
     3181 agctatgcgc ggatggctac ctcgctattg aggagttaat cccggtgccg ggtgacgtga
     3241 ccggctaccc ccagactatc cgccgctccc caccagttga tccggctcct ttcaaccaca
     3301 gcacaaaaat tgccatacta cgtatcctca ataggtcatc ccatgagtaa agagattatt
     3361 tttgaaaatg cgcgccaggt cgctttccaa gatttgccag accccccgtt gaccgccagg
     3421 caggttcggg tgcggacgct atactcgggg atcagcgctg gcaccgaaat gacagcatac
     3481 cgtggcagca acgtttacct gcataaacat tgggatgcaa aaaaccgctt gttcatctcc
     3541 gccgaaaccc cttcctcaac ttacccacta ggtggatttg gctatgagga ggttggcgaa
     3601 gtaatcgaaa ttggcgaaca ggtcacctgc ctcaagcagg gcgatatcgt atttggcacc
     3661 tgggggcacc gcacccagca tattctcgaa gaggagtatg cttcccagcg gatcaagcca
     3721 gctgagctcg agcccatctt gggcattttc tcgcacattg gaccgattgc attaaatggg
     3781 atattagatg ctcaaattca tatcggtgag aatgtcgccg tctttggcct g
//
LOCUS       DBNHOH010000092         5662 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1094085, whole genome shotgun sequence.
ACCESSION   DBNHOH010000092 DBNHOH010000000
VERSION     DBNHOH010000092.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5662)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1094085"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="AC16V7_01605"
     CDS             <1..197
                     /locus_tag="AC16V7_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255841.1"
                     /translation="DQGARMLSAIASVSSAGAVGEEEAVLAEKAQELMGTLVDPAGTF
                     YRFSLAKSLLVRSRQATHSE"
     gene            209..1705
                     /locus_tag="AC16V7_01610"
     CDS             209..1705
                     /locus_tag="AC16V7_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HIR6255842.1"
                     /translation="MKRISIDPITRLEGHGKIEIFLDEDSEVANAYFQIPEFRGFEQF
                     CVGRPAEEMPRITSRICGVCPEAHHMASTKALDALFHIEPSPAVEKLREMFYSAFYVT
                     DHTTHFYALGGPDFVLGPDAPPAERNILGVIHKVGVEIGKEVIACRQRNHHVIQMVGG
                     RGVHPVAGLPGGWSKSINEEERHEIEEIARQNVVFALFSLKIFEDIVLKNQSYLDLIL
                     SDIFTHRTYSMGTVDANNHVNFYDGLIRVVDPQGREFAKYPAKDYTKHLAEHVEPWTY
                     LKFPYLKQVGWKGFVDGEESGVYCATPLSRLNAADGMATPKAQEAFEKFYETLGSEKI
                     DGRYQPVHYRLATHWARLIELLYAAERMLELIIDPEITEPHTRRQVTEVPDEGVGCVE
                     APRGTLTHHYWTDENGILTQVNLIVGTTNNYAPIAMSVKKAAQALIHKGVDISEGLLN
                     RIEMAFRVYDPCFACATHSLPGQMPLDVTIRRLDGSILTTISREEGTS"
     gene            1702..2175
                     /locus_tag="AC16V7_01615"
     CDS             1702..2175
                     /locus_tag="AC16V7_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR6255843.1"
                     /translation="MISAKLLRDHSFFAIFNEAQLKAISEIVTYEDYQQGAEIFEEGM
                     TAEALYVLLTGRVELSYTIIPSYHPERRKESVVGEINPGEVFGISALIEPHILTMTAR
                     ATQSSRVIRFEAGELRRLMKGDQEMAYVVIDQLAKSAIQRLNATRRQLATAWEPL"
     gene            2281..2742
                     /locus_tag="AC16V7_01620"
     CDS             2281..2742
                     /locus_tag="AC16V7_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR6255844.1"
                     /translation="MISPEQLRRYPFFGRLSETQLIEIAMISDHVSFEAGEIIAKEGD
                     LAEALYFLEEGAVDLYYIPETGSILQEGYPVGTINPGEPFSISAVIEPHILTSTVRAS
                     GPCRAIRIQAQALRRLMKNDRQLAYVITHHAAKAVIERLHATRTQLAAARA"
     gene            2767..3210
                     /locus_tag="AC16V7_01625"
     CDS             2767..3210
                     /locus_tag="AC16V7_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR6255845.1"
                     /translation="MRTVKSLLDEKGNEIWSTTPDAKVIEALQILAEKDVGALLVLDG
                     EKLVGIFSERDYARKVILKGRSSKEIPVRDIMTYHVIYGHPSQTVEQCLELMTEKHVR
                     HLPILEDDQLIGIVSIGDLVKAIIAEQKKLIARLERYILQRTSLV"
     gene            complement(3234..4010)
                     /locus_tag="AC16V7_01630"
     CDS             complement(3234..4010)
                     /locus_tag="AC16V7_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255846.1"
                     /translation="MRNLSIALLSLTIFIAAGCAGQAAVLYAPNPSETPAPTATLTFT
                     PSPEPTATPLPTHTPTPTTTPTATASFTPSPSPTPTSEFPIAITTEERAFCRYGPGKA
                     YLFSHELNKGDMAEVHGRNASKTWLWLKPYNLDRHCWAAASVLEVNGDIASVNVVTSR
                     LPKSTLYESPVWVDAERDGDEVTVEWDEVWMTEDDFRGYLIEATVCQNGYLIFVPVQT
                     DDTHFTFTDETGCSGSSGGVLYTVEKHGYTDPVEIPWPDN"
     gene            complement(4275..4880)
                     /locus_tag="AC16V7_01635"
     CDS             complement(4275..4880)
                     /locus_tag="AC16V7_01635"
                     /inference="COORDINATES: protein motif:HMM:NF022747.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HIR6255847.1"
                     /translation="MNKAKSKASKRQLIRERQRKQQIISRYIWVGVGIVVLAIIGILV
                     WQTARPAVGEVVPIPISSSEHVPEDTQPGPYPSDPPAGGVHFAGTLPAKLYLEEDLAT
                     LPQYPEGYLVHNLEHGYVIFWYNCDVVGEAGCEQLITQLQEVIDSFNGFKVMAFPWKS
                     LDIPVAATSWGRLQRFESFDPGLARRFVRNNRNKAPEPNAP"
     gene            complement(5197..>5662)
                     /locus_tag="AC16V7_01640"
     CDS             complement(5197..>5662)
                     /locus_tag="AC16V7_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255848.1"
                     /translation="PEVIPPYGWLVVAAFALLILGLAIVGYILARHWSARTDYSLVGL
                     ALFLIPAIALAIGIPRASYVPVWSVLIGSLCWIATVAAGRKRKAWSVDMATVLAALPL
                     VVLFLPFLPGVVMGDGMKSLAILAAAWALLLGVVLPAIDGLLVRAPDAQGLP"
BASE COUNT         1388 a         1414 c         1567 g         1293 t
ORIGIN      
        1 cagaccaggg agccaggatg ttgtccgcga tcgcttcggt cagcagcgcg ggagcggtgg
       61 gtgaagagga ggccgtgctg gcggaaaaag ctcaagagct gatgggtaca ctggtagacc
      121 cagcaggcac attttatcgt tttagcctgg caaagtcgtt gcttgtgcgt tcacggcagg
      181 caacccatag tgaataagga gggctgctat gaaacgcatc tccattgatc ctattacgcg
      241 gctggaaggt catggcaaga tcgaaatctt cctcgatgaa gattccgaag ttgctaatgc
      301 atacttccag attcctgagt ttcgcggctt tgagcaattc tgtgtgggcc gcccggcgga
      361 ggagatgccg cgcatcacgt cacgaatctg tggtgtctgc ccggaagctc accatatggc
      421 ctccaccaaa gcgctggacg cactatttca cattgagcca tcgccggcgg tcgaaaagct
      481 gcgtgagatg ttctactcgg cgttctatgt aaccgaccac accacacact tctatgcttt
      541 gggaggcccg gatttcgtgt tgggaccaga tgcaccgccc gccgagcgca atatcctggg
      601 cgtgatccat aaagttggtg tggaaatcgg gaaggaagtg atcgcatgcc gccagcgaaa
      661 ccaccacgtc atacagatgg taggagggcg tggggttcat cccgtcgcag ggctgcccgg
      721 cggttggagc aaatcaataa atgaggaaga gcgtcatgag attgaggaaa tagcccgaca
      781 gaatgtggtc tttgccctgt tcagcttgaa gatatttgaa gatattgtgt taaagaacca
      841 gtcttatctc gacttgatcc tttctgatat ttttacccac cgcacctatt cgatgggcac
      901 ggtagatgcg aacaaccatg tcaattttta cgatggattg atccgcgtgg ttgatcccca
      961 gggcagagaa tttgccaaat atccagctaa ggactatacc aaacacctgg ccgagcatgt
     1021 cgagccctgg acatacttaa agttccctta cttgaagcag gtggggtgga aaggatttgt
     1081 ggatggggag gagagtgggg tgtattgcgc cactccgcta tcgcggttaa acgctgccga
     1141 tggtatggct actcccaaag ctcaggaagc ttttgaaaaa ttttacgaaa cgcttggatc
     1201 cgagaagatc gatgggcgct accagcctgt gcactaccgc ctagccacgc actgggctcg
     1261 cttgatcgag cttctctacg ctgcggaacg catgttggag ctgatcattg acccagagat
     1321 caccgagcct catacgcgcc gccaagtcac tgaagtcccg gatgagggcg taggctgcgt
     1381 tgaggcgccg cgcggcaccc taacccacca ttactggacg gatgagaacg gcatcctcac
     1441 ccaagtcaac ctgatcgtgg gcactaccaa caactatgct cccatcgcca tgtcggttaa
     1501 aaaggctgcc caggcgctta tccataaagg ggtggatata agcgaaggct tactcaaccg
     1561 gattgaaatg gcttttcgag tttatgaccc ctgttttgcc tgcgcaacac actcgttgcc
     1621 aggtcaaatg cctttagatg tcaccattcg caggttggat ggaagtatcc tgactacgat
     1681 atctcgagag gagggaacgt catgatatct gccaagttat tgcgcgatca ttcatttttt
     1741 gccatattta atgaagctca gctcaaggct atctctgaaa ttgtgacata tgaagattat
     1801 cagcaaggtg ctgaaatctt tgaggagggc atgacagctg aggccttgta tgttttgcta
     1861 acaggtcgcg tcgagctgtc ctacacgatt attccgtctt atcatcctga aaggcgtaaa
     1921 gagagtgttg tcggcgagat caatcctgga gaagtcttcg gcatttctgc actgattgag
     1981 cctcacatac tcactatgac cgcgcgagca acccaatcca gtcgtgtgat ccggttcgaa
     2041 gccggggaac tacgccgatt gatgaaggga gatcaagaga tggcatatgt ggtgattgac
     2101 cagttggcaa aatcagccat acagcgttta aatgccacac gcaggcagct ggctacagcg
     2161 tgggagccat tgtaaaatcc gaacaatcta gcgattgtag gtcttgccta ttgcaaaagt
     2221 agatcgcact gtatgcgtca cgcaaaaaat tcttttcact aaattaggag gaggccagcg
     2281 atgatatctc cagaacaatt acgccgctat ccattctttg gccggctaag cgaaactcaa
     2341 ttgattgaga tcgccatgat ttccgaccat gtatccttcg aggcgggtga aatcatagcc
     2401 aaagaaggag acctcgccga ggcgctatac ttcctggagg aaggcgctgt tgatttgtat
     2461 tacatacccg aaacgggatc aatccttcag gaaggatatc ccgtaggcac aatcaacccc
     2521 ggcgagcctt tctcgatctc ggcagtcatc gagcctcata ttctaacctc gacggtccgc
     2581 gcttctggac cgtgtcgggc gatcagaatc caggcgcaag ccctgcgccg cctgatgaaa
     2641 aatgaccggc aattggcata tgtgataacc caccatgcag caaaagcagt catcgagcgt
     2701 ttgcacgcca ctcgtacaca gcttgctgcc gctcgggctt agccttttat agagttagga
     2761 aacgcgatga gaacggtcaa aagcttgcta gatgaaaaag gcaacgagat ctggtctacc
     2821 accccagatg ccaaggttat agaggctctc caaattttgg cggaaaaaga cgttggggcc
     2881 ttgctggtgc tggatggaga gaagctcgtg ggtattttct ctgagaggga ttatgctcgc
     2941 aaagtgatcc tgaagggcag gtcatccaaa gaaatcccgg tcagagatat tatgacctat
     3001 catgttattt atggccatcc aagccagacc gtcgagcaat gcctggaatt gatgacggaa
     3061 aagcacgttc gccatttacc tatcctagaa gacgaccagt taattggcat cgtctcgatc
     3121 ggagatcttg tcaaggcgat cattgctgag cagaaaaaat tgatcgctcg attggaaagg
     3181 tatatcctcc agcgaaccag cctggtataa agatcggtaa ggggtggcta tatttaatta
     3241 tctggccagg ggatttccac cgggtcggta tagccatgct tttccacagt gtagagcact
     3301 ccaccagatg agccggagca ccccgtttca tcggtgaagg tgaaatgggt gtcgtcggtt
     3361 tgcacgggga cgaagatcag ataaccattc tggcacacgg tagcttcgat gaggtaaccg
     3421 cggaaatcgt cctccgtcat ccatacctca tcccactcga cagtcacctc gtctccatca
     3481 cgctctgcat cgacccagac gggagactca tagagcgtgg attttggcaa gcgcgaggtg
     3541 actacattca ccgaagcgat gtcgccgttc acttccaaca ccgaggctgc tgcccagcag
     3601 tgtctatcta ggttgtatgg cttgagccac agccaggtct tagaagcatt gcgcccgtgt
     3661 acctctgcca tatcgccttt gttgagctca tgcgaaaaca ggtatgcctt tccagggcca
     3721 tagcggcaga aggcgcgttc ctctgttgtg atcgcaatcg ggaactcaga cgtcggcgta
     3781 ggcgagggcg atggggtgaa cgaggcggtg gcggtgggag tggtggtagg cgtgggggta
     3841 tgggtgggaa gtggggtagc ggtcggctcg ggactggggg tgaatgtgag agtcgctgtg
     3901 ggtgcaggag tctcgcttgg attgggggca tagagcacag cagcctgtcc tgcgcatccc
     3961 gcggcaataa agatagttaa ggatagcaag gcaattgata aattacgcat cgcactattg
     4021 taactggact gaacaaaaat aagctctttg tattaagttc tacttaagtc ggtggagtat
     4081 atatccccat cattgggtaa gcatggctat ctgattggtc aacaacgagc tcgggaacat
     4141 ggacaatgta cttcgatcct gggtctgagg gacctgtgag caaataattt caggcgcgat
     4201 ctgaggccat ccatgccatc cgaaaattaa tagccgcgct gaatgggagt ggtgcctaaa
     4261 tcaagaatgc tcgatcaagg ggcgtttggt tcgggcgctt tgttgcggtt gttacgcacg
     4321 aagcggcggg ccaggccggg atcaaaggat tcgaagcgtt gcagccgccc ccaggaggtt
     4381 gctgccaccg gaatatccag agacttccag ggaaaggcca tcactttgaa gccattgaaa
     4441 gagtcgatca cttcctgaag ctgggtgatg agctgctcac atccggcttc accaaccacg
     4501 tcgcagttgt accagaagat cacatagccg tgctctaggt tgtgaaccag atagccctct
     4561 gggtattgcg ggagggtagc caggtcttct tctaggtaaa gtttggcagg cagtgttcct
     4621 gcaaaatgca caccgccggc tggtggatca gacgggtacg ggccgggctg cgtatcctcc
     4681 gggacgtgct cgctggacga tatggggatc ggcacgacct cgcctacggc tggtctggca
     4741 gtctgccaga ccaggatgcc aatgattgcc aatacaacaa tacccacccc tacccagata
     4801 tatctggaga ttatctgttg tttgcgctgg cgctccctga tgagctgtcg cttgctggcc
     4861 ttactctttg ccttattcat taaataacct ctttagaaca tgcgcagatg atggaaactg
     4921 gtggggaagt accccataac ttccccacca atgtaacttc tgctttttat aaggtggtaa
     4981 atccatgcat ggttgacgga ttaatttaaa ccaattttac cctaaaaagc ggtttgtctt
     5041 tgttatttaa ttgtggaatc aaaaatatgc tggcatcttg agcgcttggg aggcaggcct
     5101 gctcgcctac atcgacgatc cgcaataccc ctcaggcttg cctccttgat cgctcatggt
     5161 tgggtcgggt caagcctaat gtcagctgca ggcttgttat ggcaggcctt gcgcgtcagg
     5221 cgcgcgcaca agcaggccat cgatggcggg caacacaacg ccaagcagta aggcccacgc
     5281 ggccgccaaa atcgccaggc ttttcatacc gtcacccata acaactccgg gcaggaacgg
     5341 caggaagagc acgaccagag gcagagctgc cagtacggtc gccatatcta ccgaccatgc
     5401 ctttcgcttc ctgccagcgg ccacagtcgc gatccagcaa agcgagccga tgagcacaga
     5461 ccacacaggc acgtaggatg cgcgcgggat gccgatggct aatgcaatcg cagggatgag
     5521 gaacaatgcc agcccaacca gtgagtagtc tgtccgtgcg ctccagtgac gagccagaat
     5581 atagcccacg attgccaatc ccaggatgag gagcgcaaat gcggcgacga cgagccatcc
     5641 atagggaggg atgacctctg gc
//
LOCUS       DBNHOH010000093         6312 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_4584, whole genome shotgun sequence.
ACCESSION   DBNHOH010000093 DBNHOH010000000
VERSION     DBNHOH010000093.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6312)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6312)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6312
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_4584"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="AC16V7_01645"
     CDS             complement(<1..511)
                     /locus_tag="AC16V7_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255849.1"
                     /translation="MCYHAPVRAQTARVIEIQQDPGGFSAWLTCPAQVIPAAGQYALA
                     INPEDALQPLSMPLFPMAYSKDSFLATSPYQVAWTPGTALEIHGPLGHGFKLPPTARN
                     VALVGLGETSARLMPLIPTVLDQEGAVALFTDAPLPMLPSAVEAYPLSALPELLSWAD
                     FIAIDLPMAS"
     gene            764..1831
                     /locus_tag="AC16V7_01650"
     CDS             764..1831
                     /locus_tag="AC16V7_01650"
                     /inference="COORDINATES: protein motif:HMM:NF013324.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HIR6255850.1"
                     /translation="MNVASTIKIIASLSWFVFLGIIALAVGRASRRQPLRGATTIIIV
                     VGVLALLLSTASAGLVFVSPQERGVVITALDPGGYRPQALQAGLRWVVPFVENVVTYP
                     ISRQTYTMSIAPLEGQVQGDDSVAARTADGQEIFVDASVLYAVDPEEVVQLHIRWQNR
                     YIDDLVRPLSRGIIRDAVSQFRVEEVVTSKRFELVDQIRTNMSAKLDENGMILVDFVL
                     RNITFSPEYAASVEQKQIAEQQAQQARLVVEQRKQEAEQARQSAQGQADAAVIRAKGD
                     ADARLIQADAEAKALLLIADALQDNPELITYQYINRLAPGVQVMLVPSDNPFLLPLPT
                     LEPGVVEPSPSPSPSPPATTP"
     gene            1904..2968
                     /gene="selD"
                     /locus_tag="AC16V7_01655"
     CDS             1904..2968
                     /gene="selD"
                     /locus_tag="AC16V7_01655"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1967..1969,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HIR6255851.1"
                     /translation="MSHFNPSGRQIRLTSLSSCAGUASKLSAEALAQVLRPIKDIFNP
                     ADYPDLLVGLSDPDDAAVWRLDERRALVVTTDFFTPVVDDAYDYGAIAAANALSDIYA
                     MGGQPFLALNVAALPPDLPAELLGEILRGGAEVARQAGVVIAGGHTVQDKEPKYGLIV
                     VGFVDPERLITKSGARQGDVLVLSKPLGFGTTTTALKRGEADPTDVSEVVGWMKRLNK
                     AASELAIEAGVRGGTDVTGFSLLGHGWEMAEASGVGLRFFFGKIPFTRGARRYAHEGI
                     FPGGSADNRLYYGDHVRFDEGIDEPSQMLLFDAQTSGGLLLCVPRQKLEGLLSRAAQV
                     GQVLWEVGEVVDGEIIEVLR"
     gene            3101..4789
                     /locus_tag="AC16V7_01660"
     CDS             3101..4789
                     /locus_tag="AC16V7_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010897040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HIR6255852.1"
                     /translation="MPIHRMFGNGGYEFNSRRSPVLGRGGMVATSQPLAVAAGLEILS
                     KGGNAADAAVAVAAALNVTEPTSTGIGGDCFALYYDAATCQVSAMNGSGRAPAALTLK
                     RLQREGFGTELPPYHPYTITVPGACAGWCDLVERHGRLLLKDVLTPAIRLADEGFPVT
                     PVTAYFWDRGVTRQLGHAPGGLELTIEGRAPRPGELFRNPGLARTFRAVAQAGKQAFY
                     QGAIAEAIAATVQAAGGCLTTDDLAAHESTWEQPIHTTYRGLRVWECPPNGQGLAALI
                     ALNILEGYDLASMEPLSATRLHLQIEAMRLAFADTRWYVADPAFNPAPVDELLSKDYA
                     ASRRRLIDPARANLEFQTGTPTAASDTVYLSVVDGEGNACSFINSNYMGFGTGIVPTG
                     WGFSLQNRGHNFGLDAGHPNALAPGKRPYHTIIPGMITVDREQSSTADRQPAQANHQL
                     VASFGVMGGFMQPQGHMQVLVALADDKLDPQAALDQPRFCIQDGEAGGGVALEEGISL
                     QVKSELTLRGHPVEMVAGYDRALFGRGQIILRDAESGVLWGGSDPRADGCAMSV"
     gene            4928..5455
                     /locus_tag="AC16V7_01665"
     CDS             4928..5455
                     /locus_tag="AC16V7_01665"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HIR6255853.1"
                     /translation="MLISKELEEAINTQVGREFGASLQYNSIAAYFDADNLPSLAAFF
                     YRQASEEHMHAMKFLNYVVDAGGQVRIPAISASKYDFSSPEEAVQLALDWEQEVTRQI
                     NELMNLAIQQKDHIAQDFLRWFVTEQLEEISTMDRLLSIVRRSKDNVLFVDDYISRNP
                     IVSPEEAGGEAESGA"
     gene            complement(5510..5746)
                     /locus_tag="AC16V7_01670"
     CDS             complement(5510..5746)
                     /locus_tag="AC16V7_01670"
                     /inference="COORDINATES: protein motif:HMM:NF013286.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HIR6255854.1"
                     /translation="MVDPVYEDRLRALIERLSAYIEHYHGGSVQYISLDGSVLKVRLG
                     GACLGCPLLPSTLHGWVEGTVRQFFPEIERIEEV"
     gene            complement(5727..>6312)
                     /locus_tag="AC16V7_01675"
     CDS             complement(5727..>6312)
                     /locus_tag="AC16V7_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR6255855.1"
                     /translation="DSGSSPLEGRGKSGYSICYPGGQLNGMSSDIFDQLPLFDGFTSD
                     QRELVRPLFTPVDCYSGTVLFEQGDPADYLYLVVVGEVVIRYKPEDGPEILVARVRPW
                     GIVGWSAALGSHAYTSGAICEEYTQMLRLYGKDLRNLCEQNPETGILILERLATVIAQ
                     RLRNTHEHVMELLRQGLGTNSNYAKEGSHGRSRL"
BASE COUNT         1314 a         1734 c         1907 g         1357 t
ORIGIN      
        1 gggaagccat gggcaggtcg atggcgataa agtctgccca ggagagcagc tctggcaacg
       61 cacttagggg atacgcctct accgcggatg ggagcatggg cagcggggcg tcggtgaaca
      121 aggcaaccgc cccttcctgg tctagtacag tggggatgag cggcatcagg cgagccgagg
      181 tctccccaag gccaaccagg gcaacattgc gtgcggtggg aggtagttta aaaccgtgtc
      241 ccagcggtcc atgaatctcc agggctgtcc caggcgtcca ggctacctgg taaggcgagg
      301 tggcgagaaa actgtccttt gagtaagcca tagggaagag cggcatggag aggggttgaa
      361 gcgcatcttc ggggtttata gccagggcat attgcccggc tgctgggatg acctgcgcgg
      421 ggcaggtgag ccaagctgaa aaacccccag ggtcttgttg gatctctatc acacgggcag
      481 tttgggcgcg cacgggcgca tggtagcaca ttttgtcctg gcaggcaatg gtgtggcctc
      541 agagcagggc atatgcatat gccctgaggg gcatgcgcaa tcccgcaaaa agagtgtttg
      601 gggtgttttg gtgggggtat gcccccacca aaacacccca ttttctctcc tcagtggtgg
      661 gaaatcgccc aaatgcgatt gccctgagcc tcagagcatc agaggtgaag aaacacgagt
      721 ataatgagat cgtttacccg ttcgagtata ggaggtttta tccatgaatg tagctagtac
      781 cattaagatt attgccagcc tgtcctggtt cgtatttctg ggcatcatcg ccctcgctgt
      841 agggcgcgcc tctcgccgcc agcctcttcg aggcgcaacg accattatca tcgttgtggg
      901 cgttttggca ttgctgctct ccacagccag tgctggtctg gtgttcgtct cgcctcaaga
      961 gcgcggtgtg gtgatcaccg ccttagaccc aggaggctat cgcccgcagg cgctccaagc
     1021 cggtctgcgc tgggttgtcc cctttgtgga gaacgtggtc acatacccga tctccagaca
     1081 aacctacacc atgtccattg cccctttgga gggccaggtg cagggtgatg actcggtggc
     1141 tgcccgcaca gccgatgggc aggagatctt tgtagatgcc tcggtgctat acgccgttga
     1201 tcctgaagag gtggtgcagt tgcatatccg ctggcaaaac cgctacatcg acgacctggt
     1261 gcggccccta tcgcgcggta tcatccgcga tgccgtctcg cagtttcgtg tcgaagaggt
     1321 ggtcacctcg aagcgttttg agctggtcga tcagatccgg acaaacatgt cagccaagtt
     1381 agatgagaat gggatgattc tggtggactt cgtgctgcgc aatatcacct tctcgccgga
     1441 atacgccgcc tcggtggagc agaagcagat tgccgagcag caggcccagc aggcccggtt
     1501 ggtggtagag cagagaaagc aggaagccga gcaggcgcgc cagtctgccc agggtcaagc
     1561 tgacgcggca gtgatccgcg ccaaaggtga cgccgatgcg cgcttgatcc aggctgacgc
     1621 cgaggccaaa gctttgttgc tcatcgccga cgccctgcag gataatcccg agctgatcac
     1681 ctaccagtat atcaaccggc tggctcccgg ggtgcaggtc atgctggtgc ccagcgacaa
     1741 ccccttccta ctgccactgc ccaccctgga gccgggggtg gtcgagccgt cgccgtcacc
     1801 gtcgccctct cccccagcga caacaccgtg acctatattc taaaatacgt gcacagttga
     1861 aggagaagta cctccgcaat tgcgtaactt ctgatctgca aagatgagcc atttcaatcc
     1921 ctctgggcgt cagatccgcc tgaccagcct ttcgagttgc gccggttgag catcgaagct
     1981 gagcgccgaa gcgctggcgc aggtgctgcg cccgattaag gacatattta atcccgccga
     2041 ttatcctgac ctcttggtgg ggttgtccga tccagacgac gccgccgtgt ggcgtctgga
     2101 tgaacgccgc gccctggtcg ttacgacgga tttcttcacc ccggtggtgg acgatgccta
     2161 tgactacggg gcgatcgccg cagccaatgc cctttcggat atctatgcca tgggggggca
     2221 acccttcctg gcattgaacg tggcagccct gccgcctgat ctgcctgctg aactgctggg
     2281 ggagattctg cgcggcgggg cggaggtcgc tcggcaggca ggagtggtca ttgctggtgg
     2341 gcacactgtt caggataagg agcctaaata tggcctgatt gtggtgggct ttgtcgatcc
     2401 ggagcgcctg atcacgaaaa gcggagctcg ccagggtgat gtgcttgttt tgagcaagcc
     2461 gcttggcttt ggcactacga ccacagcctt gaagcgcggc gaggctgatc ccacggatgt
     2521 atctgaggtg gtggggtgga tgaagcgcct gaataaagct gcttctgagc tggcgatcga
     2581 ggcgggcgtg cgcggtggta ctgatgtcac tggattcagc ctgttgggtc acgggtggga
     2641 gatggctgag gcttctggcg tggggctgcg ctttttcttc gggaaaatcc ccttcacgcg
     2701 cggggcgcgc cgttatgccc atgagggtat attccctggt ggctccgcgg ataaccgcct
     2761 gtactatggc gaccatgtgc gctttgacga aggaatcgac gagccatccc agatgctgct
     2821 gttcgatgcg cagaccagcg gcggcttgct actctgcgtg ccacgccaga aactggaggg
     2881 cttattaagc cgcgctgccc aggtcgggca ggtgttgtgg gaggtggggg aggtcgtgga
     2941 tggggagatc attgaggtgc taagataagg cgcagatgtt atcgaaccct gcgggcacga
     3001 tggtgtcccg aagatgggtg gaattacaaa ataaattccc aagcgtggca aacgataaat
     3061 ttacgaaatc ccctcaaacg gggagaagag cgggtattta atgcctatac atagaatgtt
     3121 tggcaatggc ggctacgaat tcaactctcg ccggtctccg gtgttgggac gaggtggcat
     3181 ggtcgccacc tcccaacctc tggcggtggc tgctggtctg gaaatcctga gcaagggcgg
     3241 taatgctgcc gatgccgcgg tggctgttgc cgctgctctc aacgtcaccg agccaacttc
     3301 caccggcatc ggtggggatt gcttcgcttt gtactatgac gccgctacct gccaggtgag
     3361 cgcgatgaac gggtctggtc gcgccccggc tgccttgacc ctgaagcgtc tgcagcgcga
     3421 aggttttggg accgagctgc cgccgtacca tccctatacc ataaccgtgc ctggcgcctg
     3481 tgctggttgg tgcgacctgg tcgagcgcca cgggcggctt ttattgaaag atgtcctcac
     3541 ccctgccatc cgactggcgg atgagggttt cccggtgacg ccggtcacag catatttttg
     3601 ggaccgaggt gttacacgcc agcttggtca tgccccgggc gggctggagc tgaccattga
     3661 gggtcgcgcc ccccgtccag gagagctatt ccgcaacccc ggtttggcgc gcacttttcg
     3721 ggctgtagcc caggctggaa agcaagcctt ttaccagggc gccatcgctg aagccatcgc
     3781 tgcaacggtg caggcagccg ggggttgcct gacaactgac gacttggctg cgcacgagag
     3841 cacctgggag caaccaatcc atacgaccta ccgcgggctg cgtgtttggg aatgcccgcc
     3901 gaacggtcag gggctggcgg cattgatcgc attgaacatc ctggagggat atgatctcgc
     3961 ctctatggag ccgctctctg ccactcgcct gcacttgcag atcgaggcta tgcgcctggc
     4021 ttttgccgat acccgctggt atgtggcgga cccggccttc aatccagcac ctgtggacga
     4081 gctgctctcc aaggattacg ctgcctcgcg ccgtcgtctg atcgacccag cccgcgcaaa
     4141 tcttgaattc cagacgggca cgcccacagc cgcctcggat acagtctatt tgagcgtggt
     4201 agatggggag gggaacgcct gctcattcat caacagcaat tacatgggat ttggcactgg
     4261 cattgtgccg acaggctggg gattcagcct gcagaatcgc gggcataatt tcggcctgga
     4321 cgcaggtcat cccaatgccc tggcgcccgg caagcggcct tatcacacca tcatcccagg
     4381 catgattaca gtggatagag agcaatcatc cacagcagac cgccagcctg cacaagccaa
     4441 ccaccaactt gttgccagct ttggcgtgat gggcggtttc atgcagcctc agggacacat
     4501 gcaggtgctt gtagccctgg cggatgacaa gctggacccg caagctgccc tagaccagcc
     4561 acgtttctgc atccaagacg gcgaggcagg tgggggcgtc gccttagaag aaggcatctc
     4621 ccttcaggtt aaaagcgaat tgaccttgag gggacacccg gtcgagatgg tggcgggcta
     4681 tgatcgggcg ctctttgggc gcggccagat catcttgcgg gatgctgaga gcggtgtgct
     4741 ttggggtgga agtgatccgc gggctgatgg ctgcgccatg agtgtgtgag taggaacaca
     4801 ggcatcttat aaccctgcag agcgcgtgat ataatctcgc cgtgatattt ataatactaa
     4861 cgcagttgag ggaaaaaact ccccacctgc gtaaactatg attggttcaa agaattggaa
     4921 ggataacatg ttaatcagta aagaattaga ggaagctatc aatacacagg tcgggcgcga
     4981 gtttggcgcc agtttgcaat acaatagcat cgccgcctat ttcgatgcgg ataacctgcc
     5041 cagcctggca gcctttttct accggcaagc cagtgaagag cacatgcacg caatgaagtt
     5101 tctgaactat gtcgtggatg ccggtggtca ggtacgcata ccggccatct ctgccagcaa
     5161 gtatgatttc tcgtctcctg aagaggctgt ccagctcgcc ctggactggg agcaggaagt
     5221 gacgcgccag attaacgagc tgatgaatct ggccatccag caaaaagacc atatcgcaca
     5281 agacttcctg cgctggttcg tgaccgagca attggaagag atctctacca tggatcgtct
     5341 gttgagtatt gtgcgccgtt ccaaggacaa cgtgctcttt gtggacgatt acatctctcg
     5401 caaccctatc gtctcacctg aagaggcggg aggtgaggct gagtccgggg catagacgct
     5461 taaaataaaa cagcgggcac ttgtgcccgc tgttttattt taagcgtagt tacacttctt
     5521 cgatgcgctc gatttcaggg aaaaactggc gaacggtgcc ctccacccaa ccatgcagcg
     5581 tgctcggcag gagcgggcag cccaggcagg cgccgccaag gcgcactttt aataccgagc
     5641 catcaagaga tatatattgt accgacccac catggtaatg ctcgatataa gcgcttagcc
     5701 gctcgatcag ggcgcgtaat cggtcttcat agacgggatc gaccatggct accctccttt
     5761 gcgtaattcg aattagtacc caatccttgt ctgagcagtt ccatgacatg ctcgtgcgtg
     5821 ttgcgcaggc gctgtgcaat cacagtcgcc aggcgctcca ggataagtat accagtttcc
     5881 ggattttgtt cgcaaaggtt acgcaggtct ttgccgtaca ggcgcagcat ctgggtgtat
     5941 tcttcacata ttgccccaga ggtgtaggca tgactcccca gcgcggctga ccagcctacg
     6001 ataccccagg gacgcacacg cgccaccagg atttcagggc cgtcctcagg cttatagcgg
     6061 atgacgacct cgccgacaac taccagatag agataatctg ccggatcgcc ttgttcaaat
     6121 agtactgtac cggagtaaca gtctaccggg gtaaaaagag ggcgcaccag ttctcgctgg
     6181 tcagaagtaa agccatcaaa tagcggtagt tgatcgaaga tatcactcga catgccgttc
     6241 aattgacctc cagggtaaca tatactgtag ccagatttac cccgcccttc tagtggcgaa
     6301 cttcctgaat ct
//
LOCUS       DBNHOH010000094        10396 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_837846, whole genome shotgun sequence.
ACCESSION   DBNHOH010000094 DBNHOH010000000
VERSION     DBNHOH010000094.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10396)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10396)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10396
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_837846"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="AC16V7_01680"
     CDS             <1..477
                     /locus_tag="AC16V7_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/alpha barrel domain-containing protein"
                     /protein_id="HIR6255856.1"
                     /translation="PVIVHLLAQGEDEISSMVSRLENVDGVSAVEIGLPPDTSKELAI
                     RLIQAGIGELPVIARLPLERAQELAELVSQAGTAAVSLSPPRGAIPAPDGGLLRGRLY
                     GPAIYPQALLVVQALARSGIPVIGGGGVYRHADAEAMLSAGALAVQLDSVLWRGWK"
     gene            complement(562..999)
                     /gene="dtd"
                     /locus_tag="AC16V7_01685"
     CDS             complement(562..999)
                     /gene="dtd"
                     /locus_tag="AC16V7_01685"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIR6255857.1"
                     /translation="MRIVLQRVTMGRVSVNDRTVAQIGSGLVLLVGIGPNDGEEQARY
                     LAEKIANLRIFEDDQGKMNLSVLETGGEALVVSQFTLYADTRKGRRPSFTDAAMPEVA
                     SPLVERFAALLSQQGIPTQTGEFAAHMQVEIHNDGPVTIILER"
     gene            complement(1052..2050)
                     /gene="trpS"
                     /locus_tag="AC16V7_01690"
     CDS             complement(1052..2050)
                     /gene="trpS"
                     /locus_tag="AC16V7_01690"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR6255858.1"
                     /translation="MTETKAKKGRVFSGARPTGRQHLGNYLGAIQNYVALQDEYECVY
                     CIVDLHALTTLDTTENLRQNTYEMALDWLAAGIRPDETIVFVQSQVPEVTELHTILSM
                     VTPLGKLTDLPTFKEKVRMQPHNINYGLVGYPVLQTADITLYKAHLVPVGVDQAPHLE
                     FAREVVRSFNFRYKTDVLIEPQMKATETPKVLGIDGQQKMSKSLDNQIEMAATPDETR
                     ARVMQMVTDPARIRRTDPGNPDVCNVFSLHKVFSSADEVAMINVECRRAGIGCVDCKG
                     IFARNLNAHFSPFRERRAELGKDTNLVWDVLHDGKERAQEIARQTMAEVRQAVGLP"
     gene            complement(2047..2244)
                     /locus_tag="AC16V7_01695"
                     /pseudo
     CDS             complement(2047..2244)
                     /locus_tag="AC16V7_01695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
     gene            2460..3311
                     /gene="amrB"
                     /locus_tag="AC16V7_01700"
     CDS             2460..3311
                     /gene="amrB"
                     /locus_tag="AC16V7_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HIR6255859.1"
                     /translation="MAATLDVRPSPIAGQWYPADPRRLAASVDAYIDQAQLPAIEGQV
                     IAVVTPHAGHRYSGPVAGYAFAALRSLNPDLVAVVSPMHYPEASPILTTAHQAYETPL
                     GSIPVDREALHTLEKELSPSGVNISSVRHDPEHSLEIELPFLQRALTKGFRLLPLMVR
                     DPSPQVAQELGRALASVLAEQQALLVASTDLSHFFSQTVAEVLDQEILRRLAAFDPQG
                     ILQAEEEGKGFACGRGALAAVLWAAKELGGNHVQILHYATSGDVTGDYSQVVGYASAV
                     VTRKEIA"
     gene            3313..4677
                     /locus_tag="AC16V7_01705"
     CDS             3313..4677
                     /locus_tag="AC16V7_01705"
                     /inference="COORDINATES: protein motif:HMM:NF013741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR6255860.1"
                     /translation="MENPYLIDALKLLAVVLLVLANGFFVAAEFSLVSVRRTRVAELV
                     SQGVPAANWVQKAIENPDRVIAATQLGITLASLGLGWIGEPALADLLKPLVELFPATI
                     QSGISHSLSAGIAFAIITFLHVVVGELAPKSIALQSPESTALVIARPTVISEWVFKPA
                     IWLLNGSGNILLKLVGINPASGHELVHSVEELKMLVNQSAEGGIVQAEESEMLHAIFS
                     LGELVVRQVMIPRTEVIGVEADTPLEEIITLATKSTYTKLPVYEDNLDQVLGIVHVKD
                     LLRAMQSPDCDVCTARTLMREPVFVPETISVSALLGQFRENRQHIAIVLDEYGGTAGL
                     VTLEDLLEEIVGEVSDPFDRINPEIQTLADGSALIDGLALIEEVNHHLNLVLQDPNYD
                     TIAGYVLGKLGRIPRVGDAVEGDGVRLKVESMDGLRIARLSLMQTNPPPQPSPLDDGD
                     ERNR"
     gene            4729..7251
                     /gene="priA"
                     /locus_tag="AC16V7_01710"
     CDS             4729..7251
                     /gene="priA"
                     /locus_tag="AC16V7_01710"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication restart helicase PriA"
                     /protein_id="HIR6255861.1"
                     /translation="MPAYVEIAVNVPKVADVFHYHLPEALEGRVDVGHLVEVPFGRQR
                     VQGVVLGFVDKPAIPQTRAVLTTLDPQAVLTRAQLILAQRLAEATLSSLGAYIQLMLP
                     PGLGQQVDTLYTLNPQRSVRPGQSLSALQERLLALLGKRGPLRGRQIDSALPRIDWRP
                     VARSLVRRGLISAESILLSPSVRPKTVRTAQLACPPQVAEARLPELGRASSNALQRRQ
                     AMLRFLMREPGPVEVSWIYAESGGNLADLHKLAELNLVILGESEAWRDPLENIEFQPA
                     QPPKLTTDQQRCWDEIEQALRETATGQSAPPFLLHGVTGSGKTEIYLKAVETTLRMGK
                     QAIVLVPEIALTPQTIRRFVARFPGQVGLVHSRLSAGERYDTWRRARAGDLSVVVGPR
                     SALFTPLPELGMIVVDECHDDSYYQDDPPFYNGREAAVAYARLAGAICLMGSATPDIT
                     SAYQAAQGVWRYLRLPARILAHQEAVRQQADRLGQATRYRPLENLAETADLPPVQVVD
                     MRQELQRGNRSIFSQPLQEALARTLDHKQQAILFLNRRGTATHVFCRDCGYVLKCPRC
                     DTSLTYHSPQEALTCHYCGYQRKLPETCPSCKSRRIRQYGMGTERVEADLQGLFPQAR
                     TLRWDYETTRKKGAHEIILGHFVAGRADILIGTQMLAKGLDLPFVTLVGVVLADVGLN
                     LPDYRAAERTFQVLTQVAGRAGRSPLGGEVLLQTFQPEHYAIQAAAGHDFRSFYRQEL
                     EYRRQLRYPPFNRLVRLEYRHLRAHEAETAAHKLADQLRKWIQDEGRQATELAGPTPC
                     FFSRLAGLYRWQIILRGPDPVSLLRTRNLGEWRVEIDPPSLL"
     gene            7380..7901
                     /locus_tag="AC16V7_01715"
     CDS             7380..7901
                     /locus_tag="AC16V7_01715"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0106130 - purine phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006144 - purine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HIR6255862.1"
                     /translation="MQPTRHEVLTWNDVDRLIDHLLPQFLGEYEAMVMITRGGIIPGG
                     LLAEAMGITHLLTAAVDFPAQIEMEKARLFAWPQFIQFPEEQQIVGRRTLVVDDVWGS
                     GRTITTVRNRVEAAGGFPSTCVLHFNPYRNLFGKARPDYYAAITDAHIIYPWEINRGA
                     DRALLERSPQTPS"
     gene            complement(8015..8494)
                     /gene="sixA"
                     /locus_tag="AC16V7_01720"
     CDS             complement(8015..8494)
                     /gene="sixA"
                     /locus_tag="AC16V7_01720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HIR6255863.1"
                     /translation="MILYFLRHGLAGSRKDWRGDDFLRPLTKKGMKNMVRQADTFADL
                     DLNLDLIITSPLARAFQTADIVADELKLGEMLVQDERLSPGFGIDDLEQVLNEHAGAE
                     NLMLVGHEPDFSQTISALIGGCRVVCKKGGLARVDIFSTDPLQGELVWLIPPGVMIR"
     gene            complement(8531..9871)
                     /locus_tag="AC16V7_01725"
     CDS             complement(8531..9871)
                     /locus_tag="AC16V7_01725"
                     /inference="COORDINATES: protein motif:HMM:NF017085.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HIR6255864.1"
                     /translation="MPDNLLTREQRQYLEKLVEETADESLSRRARVLLLYDDQLATRH
                     VAEGAGLSRGRTRYWRRQFQGRGMAIFSRDQKFPDLRPTEPKDGEEQAGEPAQGKATR
                     PKKTKDGGGAPTDTRSVLDEFAAATKALGSPGVLSDDPLAEAGRKVWRYHFAMMLLHE
                     EGTLLGEDIEELHDMRVATRRMRAAFDVFGSAFAPKAIKRHLRGLRATGRALGQVRDL
                     DVFIDKAQRYQVSLSPEHQRDLEPLLEAWARDRADARREMIAYLETSQYRDFKEKFYK
                     FLSTPGAGVLPRSDNLAIPYLVREAAPVLIYNRLAAVRTFDTILDQAALEQFHALRIE
                     FKKLRYTLEYFREVLGSEAKTVISEIKSMQDHLGDLNDADVAAGILRDFLTRWDTLQA
                     ERPVAERRGPEPILTYLASRYAERQRLMLSFKEAWLNFNRPQLRQELAKAVAVL"
     gene            complement(9875..>10396)
                     /locus_tag="AC16V7_01730"
     CDS             complement(9875..>10396)
                     /locus_tag="AC16V7_01730"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306209.1"
                     /note="catalyzes the reversible transfer of the terminal
                     phosphate of ATP to form a long chain polyphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HIR6255865.1"
                     /translation="LIQREIEHQKNGEKGHLIFKMNSLVDKPIIQELYQASQAGVKVD
                     LLVRGICCLRPGLNGISENIQVTSILGRFLEHSRIFYFRNAGEEEVYLGSADLMPRNI
                     NRRVEVLFPVQERHMIRYLRYDVLATCLADNLRARRMLPDGTYERLKPPSKAKSVDSQ
                     AWLLNHHLHPQKG"
BASE COUNT         2200 a         3090 c         2881 g         2225 t
ORIGIN      
        1 cctgtcatcg tgcacctgct ggcacaggga gaagacgaaa tttcctcgat ggttagccgg
       61 ttggaaaatg tggatggagt aagtgctgtg gagatcggcc tgccgcctga taccagtaag
      121 gagctagcaa ttcgtttgat ccaagccgga attggggagc tgccggtgat cgcccggctg
      181 ccgttggagc gcgcacagga actagcagag ctagtttctc aagcaggcac tgcagcagtc
      241 agcctaagcc cgccacgcgg cgccatcccc gcccctgatg gcgggctgct gcgcggccgc
      301 ttgtacggtc cggcgattta cccccaggca ttgctagttg ttcaagcgct tgccaggtcc
      361 ggcatccccg tgatcggagg cggcggtgtt taccgccacg cagatgccga ggctatgctt
      421 tcggcgggtg cactggctgt tcagctcgat tcagtgctgt ggcgaggctg gaaataaccc
      481 acacccttct ccaaggatgc aggaaagcaa ggctggatgc attgttatcc aaccgactca
      541 ttgggggcgc tatccccatt tttaccgttc caagatgatg gtgactggcc catcattatg
      601 gatctccacc tgcatatggg cggcaaactc accagtttga gtggggatgc cctgctggct
      661 cagcagcgca gcgaagcgct ccaccagcgg gctggcgacc tccggcatgg cagcatccgt
      721 aaaagacggg cggcgacctt tgcgcgtgtc ggcgtacagc gtaaactgcg aaaccaccag
      781 cgcctcgccg cctgtctcca gtaccgagag attcattttt ccttgatcat cttcgaagat
      841 acgcaagttg gcaatcttct ctgccagata gcgcgcctgc tcttccccat cgttcggacc
      901 aatgcccacc aggagcacca gaccagagcc aatctgggcg acggtgcgat cattgactga
      961 tacgcgaccc atagtaacgc gttgcaacac aatccgcatg ggcgtgtaag gggataatgg
     1021 cttcccttat ccccttatct caattcccct atcaaggcaa tcccaccgct tggcgaactt
     1081 ccgccatcgt ctggcgggcg atctcttgcg cccgttcctt cccatcgtgc agcacgtccc
     1141 aaaccagatt ggtgtctttg ccgagctcgg cgcggcgctc gcggaaaggc gaaaaatgag
     1201 cgttcaaatt gcgggcgaaa atccccttac aatccacaca gccgatgccc gcccgccggc
     1261 actccacatt aatcatggcg acttcatcgg cagatgaaaa caccttatgc aacgagaaca
     1321 cgttgcacac atccggattg cccgggtcag tacggcggat gcgggccggg tcggtgacca
     1381 tctgcatcac ccgcgcccgg gtttcatccg gtgtggcagc catctcaatc tggttatcca
     1441 ggctcttgct cattttttgc tgcccatcga tgcccagcac cttgggtgtc tctgtggctt
     1501 tcatctgcgg ttcgatgagc acatccgttt tgtagcggaa gttaaacgat cgcaccacct
     1561 cgcgggcgaa ctcgaggtgc ggcgcctggt caacccccac cggcaccaga tgcgctttgt
     1621 acagggtgat atcagcagtt tgcaacacgg ggtaaccgac cagaccatag ttgatattat
     1681 ggggctgcat gcgcaccttc tctttgaacg tcggcagatc agtcagcttg cccaatggcg
     1741 taaccatgga aagaatcgtg tgcagctcag tcacctcggg cacttgcgat tggacaaata
     1801 caatggtctc atccgggcga atacccgctg ccagccagtc gagggccatc tcgtaggtat
     1861 tctggcgcaa gttctccgtg gtatcaagcg tggtgagagc gtgcaggtca acaatacaat
     1921 acacgcattc atactcgtcc tgcaacgcca catagttttg gatggctccc aggtaatttc
     1981 ctaagtgctg gcggcctgtt gggcgagccc cagaaaacac ccgacctttc ttcgccttgg
     2041 tctcagtcat aaattctccc gaagcagatt gcgcaccaac cccactactt caccgggttc
     2101 ggcgtggcgc ctggtttgca tcttagaata aatcaactcg ttgttcacgg tgacctcaaa
     2161 acgcccccca tccgacgggt acagggtaag cgattcgatc tcaggttcaa actctttgac
     2221 aagttcggct accaggccga cgacccggtc agcgtagtgt catactgcgc agtacgtgat
     2281 ctgaatattg agcaacataa taacacctcg aatagacact attaaaacac aacgttgtgc
     2341 taaaatcaat tcagtaacca aaattagatt ggtagggcag cgcaattcac gcaggcactt
     2401 tcacaattca aaccaatccg aatcccgcat cccctgcctc ttatctcctg gaggttgaga
     2461 tggccgctac gctcgatgta cgcccctctc ctattgccgg tcaatggtat ccagcggatc
     2521 cacggcggct agccgccagc gtggacgcgt atatcgacca ggctcaattg cccgctatcg
     2581 agggtcaggt gatcgctgtg gtaaccccgc acgccggtca tcgctattct ggccctgtag
     2641 ccggctacgc gtttgctgca ttacgcagct tgaatccaga tctggtggcg gtggtttcgc
     2701 ccatgcacta cccggaagcc tcacctattc taaccaccgc ccatcaagcc tatgaaacac
     2761 ccctcggttc catccctgtc gatcgcgagg cgctgcatac cctggaaaag gaactaagcc
     2821 catcaggagt caacatttca tccgttcgcc acgacccaga gcactcgctg gagatcgaac
     2881 tgcccttctt acagcgggct ctaacgaaag gtttccgcct gctgccattg atggtgcgcg
     2941 accccagccc tcaggtagca caggaattgg ggcgggcgct ggctagtgtg cttgctgagc
     3001 agcaagcgtt gctggtagct agcacggacc tatcccattt cttttcgcaa acggttgccg
     3061 aggttctaga ccaggagata ctgcgccggc tagcagcttt cgatccgcag ggtatactac
     3121 aagcggagga ggagggcaag ggctttgctt gcgggcgagg tgcgctggct gcggtgctct
     3181 gggcagcgaa agagttgggg gggaaccacg tgcaaatctt gcactacgcc acctccggag
     3241 atgtgaccgg ggattacagc caggtagtgg ggtacgcttc tgcggtggtc acaagaaaag
     3301 agattgcatg atatggaaaa cccttattta attgatgcgc ttaaactttt ggcagttgta
     3361 ttattggtgc tggcaaacgg tttcttcgtt gcagccgagt tttctctggt cagtgtgcgc
     3421 cgcacgcgcg tcgccgagct ggtatcccag ggtgtcccag cggcaaactg ggttcagaaa
     3481 gccattgaga atccggacag agtgatcgcc gctacccagc ttggtatcac cctagccagc
     3541 ctcgggctgg gctggatcgg ggagccagct ttagctgatt tgttgaaacc ccttgtggag
     3601 ctcttcccag ccaccatcca atccggaatc tcccacagcc tttcagccgg gatagcgttt
     3661 gcgatcatca catttctcca cgttgtagtc ggcgaactag cgcctaaatc catcgccctg
     3721 caaagtccag agagcactgc cctggtcatc gccagaccaa ctgtcatttc tgaatgggtt
     3781 tttaagccag ccatctggct tttgaacggc agcggcaata tcctcttaaa gctggttggg
     3841 atcaacccgg cctccgggca tgagctggta cactcggttg aagagctcaa gatgttggtc
     3901 aatcagagcg ctgaaggggg cattgtccag gcagaagaga gcgaaatgct gcatgccatc
     3961 ttcagcctgg gcgagctggt ggtgcgccag gtgatgatcc cccgcacgga ggtgatcggc
     4021 gtggaggctg acacgcccct ggaagagatc attaccctgg caaccaagtc gacctataca
     4081 aaattacccg tgtacgaaga taacctggat caggtgctgg ggatcgtgca tgtcaaggat
     4141 ctgctgcggg ctatgcagtc gccagactgc gatgtatgca ccgccagaac gttgatgcgc
     4201 gagccggtct ttgtgcccga gacgatctca gtcagcgctt tgctcggtca gtttcgagag
     4261 aaccggcagc acatcgccat cgtattggac gagtacgggg ggaccgctgg cttggtcacg
     4321 ctggaagatt tgttggagga gatcgtcggc gaggtcagcg atccctttga ccgcatcaac
     4381 ccagagattc aaacccttgc cgatggctct gccttgattg acggactggc actgatcgaa
     4441 gaagtcaacc atcatctaaa cttggttttg caagacccca actacgacac gatcgcgggc
     4501 tatgtcctgg gcaagcttgg gcgcatcccc cgagtaggcg atgcggtaga aggagacggt
     4561 gtacgcctga aagtggagag catggacggg ttaagaatcg cccgtctatc cctgatgcaa
     4621 accaatcccc caccccagcc atctcccctg gatgatgggg atgagagaaa tcgctagaac
     4681 tcagcttaag catggttgct tggggatagg gacataagaa ttcgggttat gcctgcctac
     4741 gtagaaatcg ccgtcaatgt acccaaagta gcggatgtat ttcattacca tctacctgag
     4801 gccctggaag ggcgcgtgga tgtcggccat ctggtcgaag tccccttcgg tcgccagcgc
     4861 gttcagggag tggtgctggg ctttgtagac aagccagcca tcccacaaac acgcgcggta
     4921 ttaactacat tggacccgca ggccgtgctt actcgcgccc agctcatatt ggcacagcgc
     4981 ctggcagaag cgacgctttc ctcgctggga gcctatatcc agcttatgct cccgcctgga
     5041 ctgggacagc aggtggacac cctgtacacc ctcaatcccc aacgctctgt gaggccaggg
     5101 cagagcctga gcgctctgca agagcgcctg ctggccttgt tgggaaagcg cggtcccttg
     5161 cgcggccgcc agattgactc ggctcttccc cgtatcgact ggcgacctgt tgcccgctcc
     5221 ctggtacggc gcggcctgat ctccgccgaa tcgatcttgc tctctccatc tgtgcgcccc
     5281 aagactgtgc gcactgccca gcttgcctgc ccgccacaag ttgctgaggc gcgcctgcca
     5341 gagctggggc gtgcgagttc gaacgcactg cagcgccggc aagccatgct gcgcttcttg
     5401 atgcgcgagc caggtccagt agaggtgagc tggatttacg ctgaaagcgg aggtaacctg
     5461 gcagacttgc acaagctggc tgaactgaac ctggtcatcc tgggggaaag cgaagcctgg
     5521 cgcgatcctc tggagaacat agaattccaa ccagcccagc ccccaaagtt gactacggat
     5581 cagcaacgct gttgggacga gatcgagcag gctttacgag aaacagccac tggccaatcc
     5641 gcccctccct tcttattgca cggcgtcacc ggttccggta agacagagat ctacttaaag
     5701 gcagtcgaaa caacgctgcg catgggcaaa caggctatcg ttctggtgcc cgagattgcc
     5761 ctcacccctc aaaccatccg gcgcttcgtg gcgcgctttc caggacaggt gggtctggtg
     5821 cactcgcgcc tgtctgcagg tgagcgctac gacacctggc gaagggcgcg cgccggcgat
     5881 ctgagcgtgg tggttgggcc gcgcagcgcc ctgttcacac ctttgccaga gctgggtatg
     5941 atcgtggtgg acgagtgcca cgacgattct tactaccaag acgacccgcc tttctataat
     6001 ggtcgcgagg cggctgtggc ttacgcccgg ctggcaggcg cgatttgcct gatgggctca
     6061 gccacgccag atatcaccag cgcctaccag gcagcccagg gggtatggcg ttacctgcgc
     6121 ctgccggcgc gcatcctggc ccatcaagag gcagtgcgtc aacaggctga tcgattgggc
     6181 caggcgacgc gctatcgccc gctggaaaac ctcgccgaaa ccgccgattt acccccggtt
     6241 caggtggtag acatgcgcca ggagctgcaa cgcggcaacc gctcgatctt cagccagcca
     6301 ctgcaggaag ctttagcgcg caccctggat cacaaacagc aagccatcct cttcttgaac
     6361 cggcgtggca cggctactca tgtgttttgc cgcgactgcg gttatgtgct caaatgcccg
     6421 cgctgcgata catcgttgac ctatcacagc ccgcaggagg cgctcacctg tcactactgc
     6481 ggctaccagc gcaagctgcc agaaacctgc ccgtcctgca agagccgacg catacgccag
     6541 tacggcatgg gtaccgagcg ggtggaggct gatttgcagg gcctcttccc acaagcgcgc
     6601 acactgcgct gggattatga gaccacacgc aaaaaaggcg ctcatgagat catcctgggg
     6661 cacttcgtcg ctggccgcgc cgatatctta atcggcacgc agatgctggc caaagggctg
     6721 gacttaccct ttgtaaccct ggtgggtgtg gtcctggccg atgtgggttt aaacttgccc
     6781 gattaccgcg ccgctgagcg gacattccag gtgctcaccc aggtggctgg gcgcgctggg
     6841 cgtagcccgc tgggcgggga ggtgcttttg caaaccttcc agccagagca ttacgccatc
     6901 caggctgccg cgggccatga ctttcgcagt ttctatcgcc aggagcttga ataccgccgc
     6961 cagctgcgct atccgccgtt caatcgcctg gttcgcttgg agtaccgtca tctcagggca
     7021 cacgaagcag aaaccgccgc ccataaactg gctgaccagt tacgtaagtg gatccaggac
     7081 gagggccgcc aggcaaccga gctggctggc ccgacgccct gcttcttctc acgactggcg
     7141 gggctgtatc gttggcagat catcctgcgc ggtcccgacc cggtctccct gctgcgaacc
     7201 cgaaatctgg gtgagtggcg ggtagaaata gatccccctt ctttgctata ataggtgata
     7261 tttcccctca gtcccgcttt cccaagtggt gggaccaggc tcaggactta ggcagcagta
     7321 aaaaataaca ccgatctgca taattcctga agttatcttt gcacaagatg gagttcccta
     7381 tgcaacccac gcgccatgaa gtcctcacct ggaacgatgt tgatcgcttg atcgaccacc
     7441 tgctgccaca atttttgggg gaatatgagg ccatggtcat gatcacccgc ggcggcatca
     7501 tccctggcgg tctgttagcc gaagcgatgg gcatcaccca ccttttgacc gcagcagtag
     7561 acttccctgc ccaaatagag atggagaaag cccgcctttt cgcatggccc cagtttatcc
     7621 aatttcccga agagcagcag atcgtcgggc gacgcacctt ggtggtggat gatgtgtggg
     7681 gttccgggcg caccatcacc acggtcagga accgggtgga agctgccggc ggatttccat
     7741 ctacttgtgt actgcacttc aacccatacc gcaacctgtt cggcaaagcc cgaccagatt
     7801 actacgccgc gatcaccgat gcacacatta tttacccctg ggaaattaac cgcggcgctg
     7861 accgagcctt gctagagcgg tcacctcaga ccccctcctg accatgtttt gtcaggttca
     7921 cccatcccgt tggcaagaga tgaataggat ctgcgggtgc acaaatgccc gcagacccca
     7981 atgatcatca atgccttgat tatcgagggg aaatctagcg aatcatcacc ccaggcggga
     8041 tgagccacac tagctcaccc tgcaacggat ccgtgctgaa gatatccacc cgcgccaggc
     8101 cgcccttttt gcacaccacc cggcagccgc cgatcaacgc gctgatggtc tggctgaaat
     8161 ctggctcgtg acccaccaac atgagattct ctgcgccagc atgttcattc aacacctgct
     8221 ccaggtcatc gataccaaaa ccaggagaca ggcgctcatc ttgcaccagc atctcgccca
     8281 atttcagctc atcagcgacg atgtcagcag tctggaaggc gcgcgccagc gggctggtga
     8341 tgatcaggtc caaattaagg tcaaggtctg caaaggtatc agcctggcgg accatgttct
     8401 tcatcccctt cttggtcaat ggacgcagaa agtcatcacc cctccagtct tttcggctgc
     8461 ccgccagacc gtggcggagg aagtaaagga tcatgggttt tctccgaata tgcttagtac
     8521 aggggtgaat ctacaaaacc gccaccgctt ttgccagctc ctggcgcaac tgcgggcgat
     8581 tgaaattcaa ccaggcttcc ttaaaggata gcatcaagcg ctgccgctcc gcatagcgcg
     8641 aagccagata ggtcaatatg ggctcagggc cgcgccgctc tgcaaccggt ctctcagctt
     8701 gcagggtgtc ccaacgggtc agaaaatccc gcaggatgcc agcggctacg tcggcgtcgt
     8761 tcagatcgcc caggtgatcc tgcatactct tgatttcgct gatgacagtc ttggcttcgc
     8821 tccctaagac ctcgcggaag tactccaggg tgtagcgcag cttcttgaac tcgatgcgca
     8881 gagcgtgaaa ttgctccagg gcagcctgat caaggatagt gtcaaaagtt cgcacggcag
     8941 ccaaacggtt gtaaatcaac acgggcgctg cttctcgcac cagataggga atggctaaat
     9001 tatcggagcg cggcaggacc ccggccccag gcgtggaaag aaacttgtag aatttctctt
     9061 tgaaatcccg gtattgactt gtctccaaat aggcgatcat ctcgcggcgg gcatcagcac
     9121 gatcccgcgc ccaggcttcc agcagtggtt ccaaatctcg ttggtgctct ggggagaggc
     9181 ttacctggta gcgttgggct ttatcgataa agacatctag atcgcgcacc tgaccgaggg
     9241 cacggcctgt ggcgcgcaag cccctgagat gccgcttgat cgctttgggt gcaaaggcac
     9301 tgccaaacac gtcgaaggcg gcccgcatgc gccgggtagc cacgcgcata tcgtgcaact
     9361 cctcaatgtc ctcgcccagc agagtgcctt cttcatggag cagcatcatg gcgaagtggt
     9421 agcgccacac tttacgcccg gcctcagcta gtgggtcgtc ggacaacacc cccggactac
     9481 ccagggcttt ggtcgcggcg gcaaattcat ccagcacgct tctagtatcg gtgggagctc
     9541 ctccgccatc tttggttttt tttgggcggg ttgccttacc ctgtgccggt tcgccagctt
     9601 gctcctcgcc atccttgggt tcggtgggtc ggagatctgg aaatttttga tcgcgggaaa
     9661 agatggccat cccccggcct tgaaactgcc tgcgccaata gcgggtcctg ccgcgagaca
     9721 ggcccgcccc ctccgccaca tggcgagtgg caagctggtc gtcatatagc aacaatacgc
     9781 gagcgcggcg gctgaggctc tcgtcggctg tttcttctac cagcttttcg aggtactgcc
     9841 gctgctctcg ggttagtaag ttatcgggca ttgatcagcc tttttgcggg tgtagatgat
     9901 ggttcaacag ccaagcttgt gaatcaaccg actttgcttt ggaaggaggc ttgagacgct
     9961 cataagttcc gtcaggcagc atgcgccgcg ctcgcaagtt atccgccagg caagtcgcca
    10021 aaacgtcata gcgcaggtag cggatcatat ggcgttcctg cactggaaag agcacctcca
    10081 cgcgccggtt aatattacgc ggcatcaggt ccgcgctccc tagatagacc tcttcctccc
    10141 ctgcattgcg gaagtagaag atgcggctat gctccaaaaa gcgtcccaaa atgctggtga
    10201 cctggatatt ctcactgatg ccgttcaaac ccggtcgcaa acaacatatg ccgcgcacca
    10261 gcagatccac cttcacacca gcctgtgagg cctggtaaag ctcttgaata atgggtttat
    10321 ctaccaagga gttcatctta aagatcagat gacccttctc gccgttcttc tgatgttcaa
    10381 tctcccgctg gatgag
//
LOCUS       DBNHOH010000095         2243 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_581451, whole genome shotgun sequence.
ACCESSION   DBNHOH010000095 DBNHOH010000000
VERSION     DBNHOH010000095.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_581451"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2188)
                     /locus_tag="AC16V7_01735"
     CDS             complement(<1..2188)
                     /locus_tag="AC16V7_01735"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR6255866.1"
                     /translation="MAKPSTMEIAPQVAKEEVNNLIELLDASPDRRWSDDELLLIEQV
                     AGQLTLALDNARLFQENLHLLEETSRRNEELTTLNKIISTASRSLELFEMLREILIQL
                     LTVLNMETGIICLADMKTRKLSIAVHHNMPNDLLDRLNADGLNDTLCRQVFEHAKVIK
                     INNPEEALSTDGTNLIENGLMSFIGLPLESRGDILGTACLFGIAPLSDDTPNLSLLTT
                     VGQQIGIAVENATLFHQTNEVLEETQRQTTNLGVLNEMGRSLTNLSDAGSVLENIYKY
                     TSELVDTTNFYIALYDSDAEFVSFPLVKESGQHKNIASRSMHKSITDHIIRNRNPYLI
                     NDDIDTRFQEIGVDDFDLQTPTKSYLGIPLIFGEQVIGMMGLQSPVLNQYTERELDLL
                     TAVARHAAIALQNVRLLEETRRRAEQLHTAAVIARDSTGTLALDLLLDRAANLIYDGF
                     KYYHVSILLLDEKHETAIVHASTGEAGKEMKKSKHSVGVGSKSVIGNVTECGLPLVIN
                     DITRDPLFYPNPLLPDTRAEIGIPLKIGQKVVGALNVHANVVNAFDTDDILVLQILAD
                     QIAIAVENARSYELSLNAVDDMRKADQLKTQFLANMSHELRTPLNSIIGFSRVILKGI
                     DGPITDLQEQDLTAIYNSGQHLLNLINDILDLSKIEAGKMELNFEDNVNIPDLIKSVT
                     STVTGLLKAKPIKLVQEIESALPNVRADPLKIRQVLINLLSNATKFT"
BASE COUNT          556 a          496 c          535 g          656 t
ORIGIN      
        1 cggtaaattt tgtggcattc gaaagcaagt tgatcaatac ctggcgaatt tttagtgggt
       61 ctgcacgaac attgggcagt gcggattcga tctcttgcac gagctttatc ggttttgctt
      121 tgagcagccc agttactgtg gatgttacac ttttgatcag gtcgggaata ttgacattat
      181 cctcaaaatt caattccatc ttgccagctt caatttttga caggtcgaga atatcattaa
      241 tcaggttgag taaatgttgg ccagagttat agatggcggt caaatcctgt tcctggaggt
      301 ctgtgattgg gccatcgatc ccctttaata tcacgcgcga gaaccctatg attgagttaa
      361 gaggagtacg tagctcgtgg ctcatgttgg ctaaaaactg tgtcttcagt tgatctgcct
      421 ttcgcatatc atcaactgca ttaagcgata actcgtatga acgagcgttc tccacggcaa
      481 tagcgatttg atccgccagg atttgaagca ccaagatgtc atcggtgtca aaggcgttta
      541 caacattcgc atgcacgttt agagcgccga ctaccttttg gccgattttc aatggaatac
      601 cgatttcggc gcgtgtatca gggagcaaag gatttgggta gaataacgga tccctggtga
      661 tgtcattgat caccaaaggt aagccgcatt cggtgacatt gccgattacc gatttggagc
      721 caacgcccac agagtgcttg cttttcttca tctcctttcc tgcttccccc gtggaagcat
      781 gtacgatggc agtctcatgc ttctcgtcca aaagcaagat tgaaacgtga taatacttga
      841 aaccatcata gattaggttt gcagcgcgat ccaggagcaa atccagagca agtgtacccg
      901 tgctatcccg agcaattacg gcggcggtat gcaactgttc agcgcggcgg cgggtctctt
      961 ccagcagtcg aacattttgg agggcaatag ctgcgtggcg agccaccgca gtcaagagat
     1021 cgagttctcg ctctgtatat tgattgagca ctgggctttg aagccccatc atcccgatca
     1081 cttgttcgcc aaagatcaat gggataccta gataggattt agttggggtt tgcaggtcga
     1141 aatcatcaac cccgatttcc tgaaagcggg tatcgatgtc gtcatttatc aaataaggat
     1201 tgcgatttcg gataatatga tcggttatgg atttatgcat agagcggcta gctatatttt
     1261 tatgctgccc tgattccttt acgagtggga atgagacaaa ttcggcatcg ctatcgtaca
     1321 gggcgatata aaaattggta gtatccacta gctcggatgt atatttatat atattctcca
     1381 ggacagaccc ggcatcggat aaattggtta gggagcgacc catttcgttt agcacgccca
     1441 agttggtagt ctgccgttgg gtctcttcta atacctcgtt cgtttgatgg aaaagtgtgg
     1501 cattttctac ggctatacca atctgctgac caacggttgt caacaaagat aaattgggcg
     1561 tgtcatcact cagaggggcg atgccaaaaa ggcatgctgt acccagaata tctccacgcg
     1621 actcgagtgg aagaccaata aatgacatta atccgttctc tataaggttg gtaccatcag
     1681 tcgataaagc ctcttccggg ttgttgatct ttatgacttt tgcgtgttca aatacctgac
     1741 ggcataatgt gtcattcagt ccatctgcat tcaatcggtc caaaagatca ttgggcatat
     1801 tgtgatggac ggcaatgctc aatttgcggg ttttcatatc agccaagcag attatcccgg
     1861 tttccatatt caggaccgtc aagagttgta ttagtatttc ccggagcatc tcgaataact
     1921 ccagggagcg acttgcggtg ctgatgatct tatttagtgt ggttagttct tcgtttcggc
     1981 gactggtttc ttctagtagg tgtaaatttt cttgaaatag cctggcatta tccaaagcaa
     2041 gcgtcaattg accagccacc tgctcaatca ataaaagttc atcgtcactc cagcgacgat
     2101 cagggctcgc gtctagaagc tcaattaaat tattaacttc ctctttcgca acttgagggg
     2161 ctatctccat agtagatggc ttcgccaaac cggtattcct gctacttggt ttggtccgcg
     2221 cccttgtttt ttgtgatctg gat
//
LOCUS       DBNHOH010000096         1325 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_966274, whole genome shotgun sequence.
ACCESSION   DBNHOH010000096 DBNHOH010000000
VERSION     DBNHOH010000096.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_966274"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="AC16V7_01740"
     CDS             <1..195
                     /locus_tag="AC16V7_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6255867.1"
                     /translation="VGQSGLLVDPYNQEEIFPALVALASHPGERERLRTMALESAKEY
                     SWSSTAMQVLEIYSQLCEEL"
     gene            347..1165
                     /locus_tag="AC16V7_01745"
     CDS             347..1165
                     /locus_tag="AC16V7_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HIR6255868.1"
                     /translation="MDDQIVAVFCLCDDMLKALGHHEDPQCQMSDAEVMTTAITAALR
                     YAGNFEHARKFLRDEGYIPKMLGKSRFNRRLHRIQNLFLTLFRLLGETWKDLNSKSIY
                     VLDSYPVASCDNYRICRSRRYRGEEWRGYQASKRRYFYGLKIHILVAEHGQPIEFFLT
                     PGSYSDTSAYRLYDFDLPEGAYITGDKAYNDYDVEDALLEAGLKMSPLRKKNSKRPIP
                     PWAFYLRSAYRKIVETTGSLIERVLPKSIHSVTPEGFELKIGLFVLACSLNFLW"
BASE COUNT          353 a          310 c          327 g          335 t
ORIGIN      
        1 gttgggcagt cgggcttgct ggtggatccg tacaaccaag aagaaatatt ccctgccttg
       61 gttgccctgg caagccaccc aggcgagcgc gaacgattga ggacaatggc tttggagagc
      121 gcaaaagaat attcctggtc aagcacggca atgcaagttc tggaaatcta ttcgcaatta
      181 tgcgaggagc tctgagccgt cagtctgcca tgcgattata gtgacatcga gcttggattc
      241 caataaggta acccaagttg ccaccttggg gtgaactgat gagaaagtcc atgtgttttc
      301 gggtaaagtt gttggtgcat aaaccacgac taaaccagga gaacacatgg acgaccaaat
      361 tgtagcagtc ttttgcttgt gtgacgatat gctcaaagcc ttgggacatc acgaagatcc
      421 acagtgccag atgagcgatg cagaagtcat gaccactgcg attaccgctg cacttcgtta
      481 tgctgggaac tttgagcacg cccgcaagtt tctgagagat gagggctaca tacccaagat
      541 gttgggcaaa agtcgcttca atcgccgttt gcatcgtatt caaaacctat tcctgacgct
      601 gtttcgtctg ttaggcgaga cctggaagga tctgaacagc aagtccatct atgtcctgga
      661 tagttacccg gttgcctcct gtgacaatta ccgcatctgc cgctctcgac gctaccgagg
      721 agaagagtgg agaggctacc aagccagcaa gaggcgctat ttctatggtc tgaagatcca
      781 tattctggtc gctgaacatg gacaaccgat tgagtttttc ctgacgccag gcagctacag
      841 tgataccagt gcctatcgtc tctatgattt cgacttgcca gaaggcgcct atatcactgg
      901 ggacaaggct tacaacgact atgatgtcga ggatgcttta ctggaagccg gattgaaaat
      961 gtcacctctg cgcaagaaaa actcgaagcg tccgatacca ccttgggcat tttacttgcg
     1021 atctgcttat cgcaagatcg tcgaaaccac gggcagcttg atcgaacggg tgctgccgaa
     1081 atctatccat tcagttactc ctgaaggatt tgagctgaaa atcggtttgt ttgtgctggc
     1141 ttgcagtctc aacttccttt ggtagcaact tgggttaatt taaacgatac aaaagcgtaa
     1201 ctgttattct cttggaataa acccgatcag caatcaaatt acaggggtat aacaccagta
     1261 atcgctgatc atcaaaatgc atgctccact atttcgacaa ttaaccattg tcgatcgagt
     1321 aagta
//
LOCUS       DBNHOH010000097         3777 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1094440, whole genome shotgun sequence.
ACCESSION   DBNHOH010000097 DBNHOH010000000
VERSION     DBNHOH010000097.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1094440"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="AC16V7_01750"
     CDS             <1..185
                     /locus_tag="AC16V7_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255869.1"
                     /translation="LGKIALELGSKVGVLGLAPYAGTFIDALTQTELRFPDEMSADEL
                     SDYRANMQEFRAELGV"
     gene            194..487
                     /locus_tag="AC16V7_01755"
     CDS             194..487
                     /locus_tag="AC16V7_01755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR6255870.1"
                     /translation="MKIRARGYLTFKEKINDQTLELDLDSISLKDFLEKLCLEQGDEL
                     RRILFDPQTEEVQRQISLLVNGRHYTHLPDRLDTALKDGDQVDIFPPIAGGDS"
     gene            697..1230
                     /gene="hpt"
                     /locus_tag="AC16V7_01760"
     CDS             697..1230
                     /gene="hpt"
                     /locus_tag="AC16V7_01760"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIR6255871.1"
                     /translation="MAQLPSDLARILIAEEAIQARVKELANEISKDLRGVESLYLVGI
                     LKGAFIFLADLARQLQIPHSVDFMALSSYGKSTTSGAVRILMDLREPIEGRHVMIVED
                     ILDTGHTLSYLYHILKGRNPASIRTCVLVRKRRESVEVPLDYLGFEIPDVWVVGYGLD
                     FADKYRTLPYIAELKQK"
     gene            1227..2537
                     /locus_tag="AC16V7_01765"
     CDS             1227..2537
                     /locus_tag="AC16V7_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255872.1"
                     /translation="MKRLPFILGGLILILLATGVRWSQPTSAAPALPFDELFIAPQGE
                     KPEQNWRVCRDLGVGNVPGLGIRRQRFRLCHSQGWEVLAYCLEPSLPAPAVGTRCSRT
                     NANTYWCGSGLQRLREYSVQETPTAAAPPSPVPTETALPTPTNSPPPPAAPPSAPPET
                     ATPERRAEATPERRARATQRVRAGGRGNFDLRSLLNLDWLAVPSKTPFQPQIATPTPF
                     QPHAPTAMIEATAQPSSSGFYGLDLADPGHRVRILIFPPNKKVNRGNPILISFIPGQT
                     CNYGDNRACVNTYQADTGTLTTFITVHSGVGSEAQAYRHAIEGTGINSAGLSLKQVRA
                     NLQALDGAEVVIMQGKRRYEGFTLVAATRIPAKFMKAYFNAPIQESLAFASTIDDDLK
                     RLSDNNLPLLIFETCGWKMPGEAWARGVTSTTASVYLGVIQKKP"
     gene            complement(2639..2848)
                     /locus_tag="AC16V7_01770"
     CDS             complement(2639..2848)
                     /locus_tag="AC16V7_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR6255873.1"
                     /translation="MDDRIVGIVKWFNSAKGYGFLGRDEGDDVFVHFSAIQMNGYRRL
                     EKDQKVEFSVEEGPKGLQAANVIPL"
     gene            complement(2984..3493)
                     /locus_tag="AC16V7_01775"
     CDS             complement(2984..3493)
                     /locus_tag="AC16V7_01775"
                     /inference="COORDINATES: protein motif:HMM:NF017262.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HIR6255874.1"
                     /translation="MSSPPVWALSLIYWLHMLATVVWIGGLAALSFLVLPSAKRILEP
                     VAYSRLLDGLQRRLDPLSWLCLVVLVGTGLFQMSANPNYQGLLAISNRWSAAIFIKHL
                     VFLGMTATSAALSWWVLPQLRRAALRRTRQLDGTDEQRLHRREALLLRLNLMLAIVIL
                     ALTALARVS"
     gene            complement(3497..>3777)
                     /locus_tag="AC16V7_01780"
     CDS             complement(3497..>3777)
                     /locus_tag="AC16V7_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255875.1"
                     /translation="GYGSDLAEKAEWCDAQEQLELALSMGPDEQASRLLEDVSNVCSG
                     GSSGGSDNSNSNDNDNSQQPAETEAPPTEEQPGEPAATEPPPDSTPAP"
BASE COUNT          881 a         1067 c          997 g          832 t
ORIGIN      
        1 tgttgggtaa gattgccttg gaactgggca gcaaagtcgg tgtattggga ctggcacctt
       61 acgcaggcac attcattgac gccctcacgc aaaccgagct gcgcttcccc gacgagatgt
      121 ctgctgatga gctgagcgac taccgcgcca acatgcaaga atttcgggca gaattagggg
      181 tatagattgg tagatgaaaa tcagagccag aggctatctc acatttaagg aaaaaatcaa
      241 cgatcaaact ctagagctgg atcttgattc gatctcgttg aaggattttc ttgagaagct
      301 ttgcctggaa cagggcgatg aattgaggcg catactcttc gatccacaaa cggaagaggt
      361 gcagcgtcaa atttcgctcc tggtgaacgg acgccactac actcatttgc cggatcgatt
      421 ggacaccgct ctgaaagacg gagatcaagt ggacatattc ccccccatcg caggaggaga
      481 tagctaaaaa cacaggatca aggaaaagcg cacttttgag taccgtatat gcggcttaga
      541 tcagggttta tataagatcg aaaataaatc ttgcgaaaac cctgctgctt ggcttgagat
      601 cactgtgcct tttttgaaaa aagtgatatg atctaagcca gcccgcaaga acaaattcgc
      661 tttctagcca aataattcaa actgggagtt gcccacatgg cccaattgcc ctctgactta
      721 gcccgcattc ttatcgcaga agaggccatc caggcccgtg tcaaagaatt agccaacgag
      781 atttccaaag acctgcgcgg ggtggagagc ctttacctgg ttggcatcct caaaggcgct
      841 tttatctttc tggctgacct ggcgcgccag ctccaaatcc ctcatagcgt ggacttcatg
      901 gcgctctcca gctacgggaa gagcaccacc tctggagcgg tacgcatcct gatggacctg
      961 cgcgagccaa tcgaggggcg acacgtaatg atcgttgagg atatcctgga taccggccat
     1021 accttgagct atctgtacca tatacttaaa ggtagaaacc cggcatcaat tcgcacctgt
     1081 gtgctggtgc gcaaacggcg tgaaagcgtg gaagtgcccc tagattatct gggctttgag
     1141 atccccgatg tatgggtggt cggctacggg ctggatttcg ccgataaata tcgaaccctg
     1201 ccatatatcg ctgaactaaa gcaaaaatga agcgcttgcc cttcatcctg gggggactga
     1261 tactgatttt gctagcgacc ggggtgcgct ggtcgcagcc cacatccgcc gcgcctgctt
     1321 tgcccttcga tgaattgttc atcgcccccc agggcgaaaa accggagcag aactggcggg
     1381 tatgccgtga cctgggggtt ggcaatgtgc ccggtttggg tatccgccgc cagcgtttcc
     1441 gcttgtgcca cagccaaggt tgggaggtgc tggcgtactg tctcgagccc agcctgcctg
     1501 ccccagcggt ggggacgcgc tgctcgcgca ccaacgccaa tacctattgg tgcggcagtg
     1561 gcctgcagag actgcgggag tattctgtgc aagagacgcc cacggcagct gcgccccctt
     1621 ccccggtgcc taccgaaact gcattgccca cccccaccaa ttcccctcct ccgccagctg
     1681 cacctcccag tgctcctcct gaaactgcca caccagagcg ccgcgccgaa gccacaccgg
     1741 agcgtcgtgc acgggctaca cagcgcgtcc gggctggcgg gaggggcaac ttcgacctgc
     1801 gtagcctgct gaacctggac tggctggcgg ttccaagcaa gacccccttc cagcctcaga
     1861 tcgccacccc cacccccttt caaccgcacg ctcctactgc gatgatcgaa gccacagcgc
     1921 aaccatcctc aagcggtttt tatggcctgg accttgccga tccaggccat cgcgtgcgca
     1981 tcttgatctt cccacccaat aaaaaggtca accggggcaa tccgatcttg atctcgttca
     2041 tcccggggca gacctgcaac tacggcgaca accgcgcctg tgtcaacacc taccaggcag
     2101 acacaggtac attgacgacc ttcatcaccg tccattccgg cgtggggagt gaggcgcaag
     2161 cctaccgaca cgccattgaa ggcacaggga tcaatagcgc ggggctgtct ctcaagcagg
     2221 tgcgggccaa cctgcaggcg ctggatggcg ctgaggtagt catcatgcag ggcaagcgca
     2281 ggtacgaggg tttcacactg gttgcagcga cgcgcatccc ggccaagttc atgaaggctt
     2341 atttcaacgc gcccatccag gaatctctgg cattcgcttc gaccattgat gacgatttga
     2401 aaaggttgtc agataacaac ctgccgctgc tgatcttcga gacctgtggc tggaagatgc
     2461 ccggtgaggc ctgggccagg ggggtcacca gcactaccgc ctcggtttat ctgggtgtga
     2521 ttcaaaagaa accctgagca agagttccct gcttgcccta tccaatacaa gcagagaaaa
     2581 taaaaacacc ctcttctact gagaaggggg tgtgttggta ttcaattgtc ttgatttatt
     2641 atagaggaat tacgttagca gcctgcaaac ctttggggcc ttcttctact gaaaattcca
     2701 ccttttgatc tttttccagg cgacggtagc cattcatctg gatggcagag aaatgcacaa
     2761 aaacatcatc gccctcgtcg cgtccgagaa agccgtagcc tttcgctgag ttgaaccatt
     2821 ttacaattcc aacaatacga tcgtccattt tttcgttctc catctcttac attagattta
     2881 cagcgtcacc cgcctgggtc taataacagt caggctgctg cgtacaacgg gcgtaagatt
     2941 atcatttttg gaaaactatg tcaagtggaa aatccttaca catttatgat accctggcaa
     3001 gggcggttaa ggccaggatg acgatcgcca acatcaggtt gagacgcagc aacagcgcct
     3061 ccctcctgtg caaacgctgc tcgtcggtac cgtcgagctg gcgagtccgc cgtaaggctg
     3121 cacggcgaag ttgaggcaat acccaccagg ataacgctgc gctggtggct gtcattccga
     3181 ggaaaaccag atgtttgata aaaatagcag ctgaccagcg attgctaatc gctaataagc
     3241 cctggtagtt gggattggcg ctcatttgga acaggcccgt ccccaccaat acaaccaggc
     3301 agagccaact caaggggtcg aggcggcgct gcagcccatc taataagcga ctgtaggcaa
     3361 ctggttccag tatccgcttg gcagaaggca acaccaaaaa tgagagcgct gccagtccgc
     3421 caatccaaac cacggtagcg agcatatgca gccaatagat caggctaagc gcccaaactg
     3481 gtggcgagct cattgcctaa ggcgcgggtg tgctgtctgg aggaggctca gtggcagccg
     3541 gttcgcctgg ctgctcttcg gttggcggtg cttctgtctc agcaggttgt tggctgttgt
     3601 cgttgtcatt gctattgctg ttatcgcttc cgccgctaga ccctccgctg cagacatttg
     3661 acacgtcttc gagcagtctc gaagcctgct catctggtcc catcgagagc gccagttcaa
     3721 gctgctcttg agcatcacac cattcggctt tttcagccag gtcgctaccg tagccga
//
LOCUS       DBNHOH010000098        10603 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1222498, whole genome shotgun sequence.
ACCESSION   DBNHOH010000098 DBNHOH010000000
VERSION     DBNHOH010000098.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10603)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10603)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10603
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1222498"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..858)
                     /locus_tag="AC16V7_01785"
     CDS             complement(37..858)
                     /locus_tag="AC16V7_01785"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HIR6255876.1"
                     /translation="MIDLAINTELARTILTGFIQTEITRAGFAKAVVGLSGGVDSALS
                     CYLAAEALGPENVLAVRMPYSASSQDSLDHAQMVIDTLGVTSTTIPITDMVEPYFKQN
                     PEASHLRRGNIMARTRMIVLYDQSEVFGGLVVGTGNKTEILLGYTTLYGDSACALNPL
                     GDLYKTQVRQLARALGMPEAIIQKAPSADLWKGQTDEGELGFTYAEVDQLLYLLVDQR
                     YTPEDCVEAGFAVDFVRAVVERVRRNHFKRIQPPIAKLSNRTIGYDFLYLRDWGT"
     gene            complement(904..1779)
                     /locus_tag="AC16V7_01790"
     CDS             complement(904..1779)
                     /locus_tag="AC16V7_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HIR6255877.1"
                     /translation="MKLTLALAQIKTSLGDVQANLDKHLALAAEAQASGVDLLIFPEL
                     SLTGYVLQDLTSTVARHPNGEDPVFRSLLEASRKLDMVVGFVDEDRRHRFYISSAYLS
                     RGQVTHVHHKVYLPTYGLFDEGRFFAWGDSVRAFDTRFGRAGILICEDFWHASPPYLL
                     WLDGADLFLFTSASPGRGLRNEPQLESAHWVEHINRAYASLFTSFIVHVNRVGYEDGL
                     NFWGGSTVFDPDGELLVKAPYHQEALTLTEIDLNQLHRTRVRLPLLRDERTALVMREM
                     ERILGQGKHDDKKRH"
     gene            complement(1776..2582)
                     /locus_tag="AC16V7_01795"
     CDS             complement(1776..2582)
                     /locus_tag="AC16V7_01795"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR6255878.1"
                     /translation="MYRPREEDHERGIIGTVIAFTLPGGLSIVSFSLSLGIGAVIGLA
                     WIAVRGKPKEIPVHLDAGLAAVLGGLVGGRIAYGIVHFAYFRDHLVDLILLPLGGLSW
                     PGAVIGVVLTLWVVALLRKQPVLSLMDALLPMATTVAISAWLACWLGGFAYGQTLDAW
                     WAIPSRDEWGVMAARLPVQLLGAILTLGLLWLVEWRQPSKAVAGQMALRWALGFSLIM
                     LFLTWLRADPAPLWLGLRLDAWAAILFLGFSTAGLYFFETRIKDFVEKEI"
     gene            2602..3210
                     /locus_tag="AC16V7_01800"
     CDS             2602..3210
                     /locus_tag="AC16V7_01800"
                     /inference="COORDINATES: protein motif:HMM:NF051402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR6255879.1"
                     /translation="MNDVDILLNPNLAYLFLVLGFLLILMAIMTPGTGLLEIGGLILL
                     ILAGWEITNLPINAWALGVLLLAAIFFLLAFRQSRPQLYLGLSILALVLGSTFLFRGE
                     TWSQPAVNPFLALTVSVLSSGFIWLAARKTLEARRISPSHSLESLIGAIGEAKTEIFE
                     DGSIQVAGELWSARSEKTIPAGSQVRVIGREGFILEVEAINQ"
     gene            3277..4182
                     /locus_tag="AC16V7_01805"
     CDS             3277..4182
                     /locus_tag="AC16V7_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011850007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR6255880.1"
                     /translation="MDIFGPGGTALCLVGAVIVIALAFVANAIRIVPEFQRLVVFRLG
                     RCIGARGPGIVFLIPIVDRAVRTDLREQVREIPHQTSITKDNAPISIDFLWYYKVLDP
                     TESVLQVGNFEAAAQGMATTTLRAVIGGIFLDEVLSEREHINNMLRTRLDDVTERWGV
                     KVTNVEIREIIPPREVQDAMNRQMSAERIRRAVVTESTGTREAAVNVAEGEKQAAILR
                     AEGSKQSAILEAEGARQAQLLKAEGFSAALEKIYSVAQTVDPKTMTLQYFETLKSIGA
                     SPSTKYIFPMEFSSLLGDFLKSRGE"
     gene            4192..4662
                     /locus_tag="AC16V7_01810"
     CDS             4192..4662
                     /locus_tag="AC16V7_01810"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6255881.1"
                     /translation="MSTVVSAVPGCSTMILPATWRDFNIIRQIEHICFPRDAWPFWDI
                     FSVLTLPNVVRLKAVCNSQTVGFIASDVRPVEKMAWIATICVLPEFRQRGIGTALLHA
                     CEGRINLPSMRLNVRASNETAVRLYKNLGYSQIGVWPRYYQDGEDALILEKRLY"
     gene            4667..5806
                     /locus_tag="AC16V7_01815"
     CDS             4667..5806
                     /locus_tag="AC16V7_01815"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:NF013755.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HIR6255882.1"
                     /translation="MSSSEHLPAPIFIARPAALERLARLLQQEAIIAVDTESNSLYAY
                     QEQVCLIQFSTLSHDYLVDPLAIKDLSSLGPVFSDPQIEKVFHAAEYDLICLRRDFGF
                     EFASIFDTMIAARILGRAEVGLGAILEAEFRVRLDKRYQRANWGRRPIPPELLAYAQL
                     DTHYLIRLRERLRRDLKEKSFWPLACEDFQRMGTTNGRPAENKGDDFWRINGARDLAP
                     QNAAVLKELWRYRDHMARVTNRPLFKVIGDRTLLAIAERCPNEAGELRQIPGMTSGQM
                     ARHGRELLAAVQKGLRAKPLYPPHLNRPDDKHLERFESLRNWRKTTARHMGVPSDVVL
                     PRDLMLLLAELNPRKPGDLSKIMEGVPWRLEHFGEQILAVLLSAQ"
     gene            5865..7262
                     /locus_tag="AC16V7_01820"
     CDS             5865..7262
                     /locus_tag="AC16V7_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6255883.1"
                     /translation="MKITFHGAARTVTGSRHLLQVNGATLLLDCGLFQGRRQESYQRN
                     LHLGFDPAKIDAVILSHAHIDHSGNLPNLVKSGFRGTIYATPATAHLGNLMLMDSGHI
                     QELDAAYVNKKRLRRGEPTIEPIYTMEDAAAVAPYFQPVNYKQRIEPIPGVTAHLVDA
                     GHILGSASVALEIEENGRKTRLWVSGDIGRRDLPLVRDPTLPEETDTLIMECTYGDKI
                     HRGPQEAYEQLREVVVRTVQRGGKVIIPAFAVGRTQELVYDLHRMIDSGEVPRIPVFV
                     DSPLAINASEIFRAHPECFDEETRQFIQEDKHQAVFGFDLLSYTRTVEESKALNERRD
                     PMVIISASGMAENGRILHHLANNIENPRNTVLIVSWQAPYTLGRRLAEKQEEVRIFGD
                     TFRRRAEVATIGGLSAHAGQNLLLEYAQRVKGSAKNIYLVHGEEKPAAVLTQLLAEDG
                     MSQVHYPQQGWSVEI"
     gene            7329..7416
                     /locus_tag="AC16V7_01825"
     tRNA            7329..7416
                     /locus_tag="AC16V7_01825"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7363..7365,aa:Ser,seq:cga)
     gene            complement(7423..8526)
                     /locus_tag="AC16V7_01830"
     CDS             complement(7423..8526)
                     /locus_tag="AC16V7_01830"
                     /inference="COORDINATES: protein motif:HMM:NF024800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase zinc ribbon domain-containing
                     protein"
                     /protein_id="HIR6255884.1"
                     /translation="MEIKIRRLYLAVRYNLLNCAHHILVDKHAQKSPGAAQENPRQQV
                     TRIVRPKGVSFRSTPPKVSEGKHEAIISEELWQRSQAVRTSRWVTVKSAKKTVRGNLL
                     QGLLVCSDCGRRLNIQTPKNCATYYRENSHHRGYHDCPYIGQSVRAVTIDAQVAELIR
                     SIYLPENWEPVVRQMLHEQRDQFYPETERKEIRTMLRLMRDNYERGMYEGEEHQYWQK
                     VNALKEKLELLNRIPEAAIDRAARTLLNLHDSWEWATREERKMLVRTMLQEVGCDVGA
                     KRIVWVKVNPDYEILFRLMDGLQPDAGRRYWIREYSAEEDIGDIGEELGQKETEVKIV
                     LPMSHNALTIVVDTVKNMYFKSLIALRSRAISE"
     gene            9001..10341
                     /locus_tag="AC16V7_01835"
     CDS             9001..10341
                     /locus_tag="AC16V7_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017745666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family RNA-guided transposase"
                     /protein_id="HIR6255885.1"
                     /translation="MNALEQINPHAAGIDIGGEEVWVAVPPDRDETSVRSFATFTADL
                     RSLASWLQACGIDTVAMESTGVYWIPLYEILEASGFRVYLVNARHLKNVSGRKTDVLD
                     CQWIQQLHTYGLLSPSFRPPEQIVALRSLVRHREMLVQYRSAHIQHMQKALTQMNLRL
                     TSVLSDITGVTGMKIIRLILAGERNPQVLAGYREPSCKKTEAEIAKSLEGHYKREHVF
                     ALKQAVELYDFYDRQIRECDAELEAMYQAFNPPDEPGTPPPTARTQKRRKNQASFDLA
                     PALYRLTGVDLTQIDGVDELTVQKVLSETGTDMSKWPTVKHFASWLRLCPNNKITGGK
                     IKQTGVQPTQNRASTALRVAASSLKYSDSALGAYYRRMRARHGAPAAITATAHKLARI
                     IYFMLRDRKPYHDFGADYYEQQHRARVVRNLNRQAVRLGFRLEPASPATPPGVS"
     gene            10431..>10603
                     /locus_tag="AC16V7_01840"
     CDS             10431..>10603
                     /locus_tag="AC16V7_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008718315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HIR6255886.1"
                     /translation="MITGVVFDIKEFAVHDGPGLRTTVFLKGCPLSCIWCHNPEGQSS
                     KPQEMRSGTGVRHV"
BASE COUNT         2415 a         3016 c         2937 g         2235 t
ORIGIN      
        1 tcatactatt ctctcttcta cagcaagcta acgtccttac gtcccccaat cacgcaagta
       61 aagaaaatca tacccgatcg tgcggttgga aagcttggcg atgggcggct gtatgcgctt
      121 gaagtgattg cggcggacac gctcgaccac cgcgcgcaca aaatcgacag caaacccggc
      181 ttccacgcaa tcttcggggg tataccgctg atctaccagc aagtataaga gctggtctac
      241 ctcagcatag gtgaagccta gctcaccctc gtcggtctgg cctttccaca ggtcagcgga
      301 gggtgctttc tggatgatcg cctcgggcat gcccagcgcc cgcgcgagct ggcgcacctg
      361 ggtcttgtac aggtcgccca gcggattcaa ggcgcaagca gaatcaccat atagggtggt
      421 atagcccagt aaaatctcgg tcttattgcc tgtgccgacc actagccccc caaagacctc
      481 cgattgatcg tataaaacga tcatgcgggt gcgcgccatg atgttgccgc gccgcaggtg
      541 gcttgcctcg ggattttgct tgaagtatgg ctcaaccata tctgtgattg ggatggtcgt
      601 cgaggtcacc cccagggtgt cgatcaccat ttgggcgtga tccagggaat cctgagatga
      661 tgcgctgtat ggcatgcgca ccgcaaggac attctctggt cccagcgctt cagcagccag
      721 atagcaagag agggccgaat caactcctcc ggaaagtccg accaccgctt tcgcaaagcc
      781 agcgcgggtg atctccgtct ggataaagcc ggtcagtatg gtgcgagcca gttcggtgtt
      841 aatcgccagg tcaatcatct taccctcatc atgctttggg ccccctatgg ttgaaatgcc
      901 gaattaatgt ctcttcttgt catcatgctt cccttgaccc aatatcctct ccatctcgcg
      961 catcaccaag gcagtgcgct catcgcgcag caagggcaag cgaacgcgcg tgcggtggag
     1021 ctggttcagg tcgatctcgg tcaaggtaag ggcttcctgg tgatatgggg ctttgacgag
     1081 caactcgcca tctggatcaa aaaccgtcga gccgccccaa aaattcagtc cgtcttcata
     1141 gcctacccgg ttgacgtgaa caatgaatga ggtaaacaag ctggcgtagg cgcggttgat
     1201 gtgctccacc cagtgcgctg actcgagctg gggttcgttg cgcagaccgc gaccgggcga
     1261 agccgaggta aacaggaaca ggtccgcccc gtccagccag agcaggtaag gtggcgaggc
     1321 atgccagaaa tcctcacaga ttaaaatacc agcgcgccca aagcgagtgt caaaggcgcg
     1381 taccgaatcc ccccaagcga aaaaacgtcc ctcatcaaag agtccgtagg tgggcagata
     1441 caccttgtgg tggacatggg tcacctgccc gcgggagagg taagccgaag agatgtagaa
     1501 gcggtggcgc cgatcctcat caacaaagcc gaccaccatg tccagcttgc gactggcttc
     1561 taagaggctg cgaaagactg ggtcttcccc atttggatgg cgggctaccg tagaggtgag
     1621 gtcctgcagg acgtaaccgg tcagggagag ctcaggaaag atcagcagat ccacgccgct
     1681 cgcctgcgct tctgccgcca gcgccaggtg tttatccagg ttggcctgga catcacctag
     1741 ggaggtcttg atctgagcga gggcgagggt gagtttcata tttccttctc aacgaaatct
     1801 ttaatacgcg tctcaaagaa gtacaaacct gcagtgctaa accccaaaaa tagaatagcc
     1861 gcccaggcat ctagacgcaa cccaagccag agcggcgctg gatccgcgcg caaccacgtg
     1921 agaaagagca taatcaaaga aaagcctagc gcccatcgca aggccatctg ccccgcaacc
     1981 gccttgcttg gctggcgcca ttccaccaac catagcaaac ccaaggtcag gattgccccc
     2041 aggagttgta cgggcaggcg ggcagccatc acaccccact cgtcgcggga aggtatagcc
     2101 caccacgcat ctagggtttg accataagcg aagccgccca accagcacgc tagccaggcg
     2161 ctaatggcaa cagtcgttgc catcggcagc agggcgtcca tcagggataa aacgggctgc
     2221 tttcggagga gcgcaactac ccataacgtc agcaccaccc caatgaccgc gcccggccaa
     2281 gagagaccgc ccagaggcaa caggatgaga tcgaccaagt ggtcacgaaa gtaggcaaaa
     2341 tggacaatgc cataggcgat tcgcccgcct acgagacccc ccagcacagc cgccaaaccc
     2401 gcatcgaggt gaaccggaat ttcttttggc tttccccgca cagcaatcca ggcgagacca
     2461 attactgccc ctatccccaa agataaagag aaagaaacaa tggacaagcc acctggcaag
     2521 gtgaatgcaa tcacagtgcc aatgatacca cgctcatgat cctcctcgcg gggtcgatac
     2581 atgttgatgt ataatgagca tatgaatgac gttgacatat tgctcaatcc caatctggca
     2641 tacttattcc tggtgctggg ctttttgctg atcctcatgg caataatgac ccccggaacc
     2701 ggtctgctcg agattggtgg actcattctg cttattttgg ccggctggga aattaccaac
     2761 cttccaatca acgcctgggc gctgggtgtc ttgctgctgg cagccatttt cttcctgctg
     2821 gcatttcgcc aatccagacc ccagctatat ctgggtttgt ctatcttggc cctggtgctt
     2881 ggctccacgt ttctatttcg tggggagact tggagccagc cagcagtaaa ccctttcctg
     2941 gcgctgaccg tatccgtgct cagcagcggg tttatctggc ttgctgcacg caaaacgctt
     3001 gaagcccggc gcatatctcc ctctcacagt ctagagagct tgattggggc cattggtgaa
     3061 gccaaaacgg agattttcga agatggttcc attcaggtag ccggtgagct gtggtctgcc
     3121 cgcagcgaaa agaccatccc cgctggctct caggttcgcg tcatcgggcg ggagggcttt
     3181 attttggaag tagaggcgat caaccaataa acatcgaaac cccaaaaggt ccctgagact
     3241 ttttgggtct caacctaaaa acactggagg tcaatcatgg acatatttgg acccggaggg
     3301 actgctctat gcctggttgg agcagttatc gtcattgccc tggcttttgt tgccaacgcc
     3361 atccgcatcg ttcccgaatt ccagcgcctg gtcgtcttcc ggctgggtcg ctgcattggg
     3421 gcgcgcgggc cggggatcgt cttcctcatc ccgatcgtag accgggcagt gaggaccgac
     3481 ttgcgcgagc aagtgcgtga aatccctcat cagacatcga tcaccaaaga caacgccccg
     3541 atatcgatcg atttcttgtg gtactacaaa gtgcttgacc caaccgagag cgtcctgcaa
     3601 gtgggcaact ttgaagcggc tgcccagggt atggcaacca ccaccctgcg ggcggtcatc
     3661 ggtggtattt tcctggatga agtgctgtca gagcgtgagc acattaacaa catgctgcgc
     3721 actcgcctgg atgacgtcac cgagcgctgg ggagtgaaag tcaccaatgt ggagatccgc
     3781 gagatcatcc cgccgcgtga agtgcaagat gcgatgaacc gccagatgtc tgctgagcgc
     3841 atccgccgcg ccgtggtgac cgaatcgacg ggcacgcgcg aggccgcggt aaacgtggct
     3901 gagggtgaaa agcaggcggc tattttgcga gcagagggct cgaagcagtc agccatcctt
     3961 gaggcagaag gcgctcggca ggcacaattg ctgaaggctg agggcttctc cgccgccctg
     4021 gaaaaaatct actctgtagc ccaaacggtg gaccccaaga caatgacgct gcaatacttc
     4081 gaaactctga agtcaatcgg cgccagccca tcaaccaaat atattttccc gatggagttt
     4141 tccagcctct tgggtgattt tctcaagagc cgaggagaat aaacccgcct gatgagcacc
     4201 gttgtatctg ccgtgccagg ttgctcgacg atgatcttgc cggccacctg gcgcgacttt
     4261 aacatcatcc gtcaaatcga acatatttgc ttcccgcggg atgcctggcc cttctgggac
     4321 attttcagcg tattaaccct gccaaacgtg gtgcgcttga aagctgtttg caacagccag
     4381 acggtgggat tcatcgccag tgacgttcgt ccagtggaga agatggcctg gatcgctacc
     4441 atctgtgtct tgccggaatt ccgccagcga ggcattggca cggcactgtt gcatgcctgc
     4501 gagggtcgca tcaacctacc cagcatgcgc ctaaatgtgc gcgcctcaaa tgagacagct
     4561 gtacgcctat ataaaaacct ggggtattcc caaatcggcg tttggccgcg ctattaccag
     4621 gacggagagg acgctttgat actggagaaa aggttatatt aagtttatga gttcctcaga
     4681 acacctgcca gctccgatct tcatcgctcg tccagcagcc ctagagaggc tggcaaggct
     4741 gttgcagcaa gaggcaatca tcgccgtcga caccgaatcg aacagcctgt atgcctatca
     4801 agagcaggtg tgtttgatcc aattctccac gctcagccac gattacctgg tagacccgct
     4861 agctatcaag gacctctcca gcctgggccc cgttttcagc gatccgcaaa tcgagaaggt
     4921 cttccatgcc gcagagtatg acttgatctg cctgcgcagg gatttcggat tcgagttcgc
     4981 cagcatcttc gacaccatga ttgcagcgcg catcctgggg cgcgccgagg tgggccttgg
     5041 ggcaatactg gaagccgagt tccgcgttcg cctggacaag cgctaccagc gggcaaactg
     5101 gggaaggcgg ccgattcccc cggagttatt agcctatgcc caactggaca cgcactacct
     5161 tatccgcctg cgggaacgcc tgcggcgtga cttgaaggag aagtcttttt ggcccctagc
     5221 ttgcgaggat ttccagcgta tggggaccac aaatgggcgc cccgctgaaa acaaaggcga
     5281 tgatttctgg cgcatcaatg gcgcgcgcga tttggctcct cagaacgcag cggtgttgaa
     5341 agagctatgg cgctaccgtg accatatggc ccgcgtgaca aaccgcccgc tattcaaggt
     5401 aattggcgac cgcacgctgc ttgctattgc agagcgttgc ccaaacgagg cgggtgagtt
     5461 gagacagata cctggaatga ccagcggcca gatggcgcgg catggtcgag agctgctggc
     5521 agccgtgcaa aaggggttgc gggcgaagcc tctctatccg ccacatctca accgcccgga
     5581 cgataagcac ctggagcgct tcgaatcgct gcgtaattgg cgcaaaacca ccgcgcgcca
     5641 catgggcgtg ccatcggacg tggtattgcc acgcgacctg atgctgttac tggcagaact
     5701 caacccacgc aagccgggtg accttagcaa aatcatggag ggagtgccct ggaggctgga
     5761 gcacttcggc gagcagatat tagcggtgct tttatccgct caatagacgt gcttcagtcc
     5821 acagtaccta ctccatccat ccaaatgcga aaatcctaaa tcttatgaaa atcacctttc
     5881 atggagcggc ccgcacggtt accgggtccc gccacttact acaggttaac ggcgccacct
     5941 tgctcctgga ctgcggcctg tttcaaggtc gccggcagga gtcctaccaa cgcaacctgc
     6001 acttggggtt cgacccagca aaaattgatg cggtcatctt gtcccacgcc catatcgatc
     6061 atagcggcaa cctgcccaac ctggtgaaga gcggcttccg cggcacgatc tacgccacgc
     6121 cagccaccgc gcacctgggt aacctgatgc tgatggactc tggtcacatc caggagttgg
     6181 acgcggccta tgtcaacaaa aagcgattaa ggcgcggcga gccgaccatc gagccgattt
     6241 atacgatgga agatgcggcc gcagtggcgc catattttca gccggtaaat tacaaacaac
     6301 gtattgagcc aattccaggt gtgacagccc acctggtgga cgccgggcac atcttgggct
     6361 cggcatctgt agccctggaa atcgaagaga acgggcgcaa gactcgcctg tgggtctccg
     6421 gggatattgg acggcgcgat ttgccgctgg tacgcgaccc gaccctgccc gaggagaccg
     6481 atacgctgat catggaatgc acctacggcg ataaaatcca ccgcggcccg caggaggcct
     6541 acgagcagct gcgcgaggtg gtagtgcgca cggtgcagcg cggcggcaaa gtgatcatac
     6601 cagcctttgc cgtgggacgc acccaggagc tggtgtacga cctgcaccgc atgatcgaca
     6661 gcggcgaggt gccgcgcatc ccggtcttcg tggacagccc gctggcgatc aacgcttcgg
     6721 agatcttccg cgcccacccg gaatgcttcg acgaggagac acgccagttc atccaggagg
     6781 acaagcacca agctgtcttc ggcttcgacc tgctcagcta cacccgcacc gtggaagaat
     6841 ccaaagccct gaacgagcgc cgggacccga tggtgatcat ctcagcatcg ggaatggcgg
     6901 aaaacgggcg catcctgcac cacctggcga acaacatcga aaatccgcgc aacacggtgc
     6961 tgatcgtctc ctggcaggct ccgtatacct tgggccgccg cctggcagag aagcaggaag
     7021 aggtgcgcat cttcggcgac accttccggc gcagggcgga ggtggctacc atcggcggcc
     7081 tgtcggcaca cgccgggcaa aacctactgc tagaatacgc ccagagagtg aaaggcagcg
     7141 ccaagaacat ctacctggtg catggtgagg agaagcccgc ggctgtgttg acccagctac
     7201 tggcggaaga cggcatgagc caggtgcatt atccacagca aggatggagc gtggaaattt
     7261 aggccgatct acgctataat cgcggtaagt ccgtgtcaag tgttcccgtg ccacgtgaca
     7321 cgttgattgg agaggtgcca gagcggccga ttggggcggt ctcgaaaacc gttgtggtcc
     7381 ttgcgaccac cgtgggttcg aatcccaccc tctccgctct cttcattcgc tgatcgccct
     7441 gcttcgcaag gcgatcaggc ttttaaagta catattcttt acagtatcca cgacgattgt
     7501 caaggcattg tgggacatcg ggaggacgat tttgacttcg gtctccttct gtcccaactc
     7561 ttcccctatg tcaccaatgt cctcttccgc actatattcc ctgatccagt agcgtcgccc
     7621 ggcgtcgggt tgcagtccat ccatcagcct gaaaagaatc tcgtaatccg gattgacctt
     7681 cacccacaca atacgcttgg caccaacatc gcaaccgact tcttgaagca tagtgcgcac
     7741 caacatcttg cgctcttcgc gggtcgccca ttcccaactg tcgtgcagat tcagcagggt
     7801 acgggctgcc cggtcgatgg ccgcttcggg gatgcggttg agcagctcaa gcttctcttt
     7861 caaggcgttg accttctgcc agtattggtg ctcttcccct tcgtacatgc cgcgctcata
     7921 attatctcgc atcaaacgca gcatggtgcg gatctccttg cgctctgtct ccggataaaa
     7981 ctgatcacgt tgttcatgga gcatctgacg cacaaccggc tcccagttct ccggaaggta
     8041 gatggatctg atcagctctg ctacctgggc atcgatggtt actgcgcgaa cactttggcc
     8101 gatgtaagga caatcgtgat aaccgcgatg gtgggagttc tctcgatagt aggtggcaca
     8161 attcttgggt gtttgaatgt tcaatcgcct accacaatcc gagcaaacaa gcagcccctg
     8221 gagcaggttg ccccgcactg ttttcttggc agatttaact gtcacccacc ggctggtgcg
     8281 tactgcctgg gaacgctgcc atagctcctc gctgatgatc gcttcgtgtt tcccttctga
     8341 aaccttgggt ggcgttgaac ggaagctgac gcctttgggg cgcactatcc gcgtgacttg
     8401 ctggcggggg ttttcctggg cagcgcctgg ggacttctgg gcgtgcttgt cgacgagtat
     8461 gtgatgagca caatttagta aattgtatcg aaccgcaaga tacagccttc ttatcttgat
     8521 ctccaccaac aggagtaggc aaacatcccc tgaccgatca gggctttcac aaagtagctt
     8581 gacgcaccca agtttctatg gcgcgcaaat cgcaatcgct gaaaacaagt gccgccttgc
     8641 gtaagcattt ctgctgtggc gtatgaaatt taccgatttt tctcgatatc gcacgaaaag
     8701 caagttgtct gactttttct tgtgaacaat accacagcgc caaggcgctc ttctttctgg
     8761 aagaaacctg agtggaaggg tagaattcat ttccagccca gacattgaga ccaacaggct
     8821 aatggtgtct gcgaacccgt ttgtgaacat ggttccgatt atcgtggttt tttcattggt
     8881 ggcccatctg ggaccccgtt taggaacgta cccaatctct tgataatcgg gtctgggcga
     8941 tccacttttc cagaggagca agagatggct aaacagaaac agcagaagca gaaagttcag
     9001 atgaatgcgc tggagcaaat caatccgcat gccgctggga tcgacatcgg aggggaagaa
     9061 gtctgggtag ctgtgccacc cgaccgagat gaaaccagcg tgcgttcctt tgcgaccttc
     9121 actgcggacc tgcgcagctt ggcgagttgg ctgcaagcct gcgggatcga tacggtggcg
     9181 atggaatcga ccggagtata ctggatcccg ctgtatgaga tcctggaagc cagcgggttt
     9241 cgggtatatc tggtcaatgc ccggcatttg aagaatgttt ctgggcgcaa aacggatgtt
     9301 ctggactgcc aatggatcca acagttgcac acctatggct tgctgagccc gtccttccgc
     9361 ccgcctgagc aaattgtggc tttgcgcagc ctggtgcgcc atcgcgagat gctggtgcag
     9421 tatcgttcgg cgcacattca gcacatgcag aaggcgctca cccagatgaa cctgcgcctg
     9481 accagcgtgc tcagcgacat cactggggtc accggcatga agatcatccg cttgatcctg
     9541 gcaggcgagc gcaacccgca ggtcttggct ggctatcgcg agccgagctg caagaaaacg
     9601 gaagccgaga tcgccaaatc gctggaagga cactacaagc gcgaacatgt gtttgccctt
     9661 aagcaggctg tggaactgta tgatttctat gaccggcaga tacgggaatg tgatgccgag
     9721 ttggaagcga tgtaccaggc attcaaccca cccgatgaac ccggcacacc gccgccgact
     9781 gcccgcaccc agaagcggcg caagaaccaa gcctcctttg acctggctcc agccctgtat
     9841 cgcctgacgg gtgtggactt gacccagatc gatggggtcg atgagttgac agtccagaaa
     9901 gtattgagtg aaaccggcac cgatatgagc aaatggccca ccgtgaagca cttcgcttcc
     9961 tggctgcgct tgtgtcccaa caacaaaatc acgggcggta agatcaaaca aaccggggtg
    10021 cagccgaccc agaaccgggc cagcacggct ttgcgggtgg ctgcttccag tttgaaatat
    10081 agcgatagcg ctttgggagc ctactaccgg cgcatgcggg ctcgacatgg cgcaccagct
    10141 gccatcacgg ccacagccca taaactggcc cgcatcatct actttatgct gagggatcgt
    10201 aagccttatc atgacttcgg cgccgattat tatgagcagc aacatcgagc tcgagtagta
    10261 cgcaatttga accgccaggc tgttagactt ggtttccgtt tggagcctgc aagtccggct
    10321 accccaccag gagtttctta ggaagccctg tgggcaggaa ctgattcggg ataaaatatg
    10381 acgagacggg ttacactgta acacgttttt caaaacactt gagattggaa atgatcacag
    10441 gagttgtctt cgatatcaag gagtttgctg tacacgatgg acctggattg cgcaccacgg
    10501 tctttctaaa gggttgccct ctttcttgta tttggtgtca taatcctgag ggacagtcga
    10561 gtaaacccca ggaaatgcgg agcgggaccg gtgttcgcca tgt
//
LOCUS       DBNHOH010000099         2904 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_68820, whole genome shotgun sequence.
ACCESSION   DBNHOH010000099 DBNHOH010000000
VERSION     DBNHOH010000099.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_68820"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="AC16V7_01845"
     CDS             <1..287
                     /locus_tag="AC16V7_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255887.1"
                     /translation="PASAPHLDAVYAWVNYIEQGDMFWMMLRDFPYTVPNAAALEYAK
                     ENETELYDAYINSPITNTPPEVLANGFFMEDVGEATPLYDQIWTEVKGEE"
     gene            351..2261
                     /locus_tag="AC16V7_01850"
     CDS             351..2261
                     /locus_tag="AC16V7_01850"
                     /EC_number="1.2.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF013481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HIR6255888.1"
                     /translation="MAYGYNGKILHVDLTHGTLEIEEPPESFYRKYMGGSAMGLYYIL
                     RDMPAGVDPLSPDNILTLMASVTTGAAISGQSRINANAKSPMGGAIGDSQSGGFFPAE
                     LKFAGFDGIVVKGRSEKPVYLSILFGEVALHDASHLVGKVTGEVDALLRDEVNEPKAE
                     VLQYGPAAENGVRFSSLVSMANRNNGRTGMGLVMASKNLKAIVVRGNAKVQIADPKKL
                     VELNRSGPKLLPENPDVDALFQHGTANVLMPQNTTGTLPTFNYNEGQFEFCEDISGER
                     MTETILKDRDTCYACVVRCKRVVEITEGAYKAYPLYGGAEYETLSTFGSYCGVKDLEA
                     ISLANQICNMYGVDTISCGATIAFAMECYEKGIIGSSETGGIELRFGNTQAMLDTLQQ
                     IVENTGPLGKVLSQGSERAAKVWGPAAEDCLITVKGEEAPAHMPQAKKSLALIYAVNP
                     FGADHQSSEHDPMYEDDCLPLYLERLAELDLKDVPPYGSLGREKVRFATYTHIFYSLL
                     DTLELCQFVWGPAWTLYGPSEIVEFVRAVTGWNVSLFELVKVGQRRLNMLRAFNAREG
                     FSRKDDKLPKKFYKALQGTGPTAGVAIDPAEFEAALDMYYQLLAWTPDGTPTRTALAD
                     LGIEWVADTVGV"
     gene            2362..>2904
                     /locus_tag="AC16V7_01855"
     CDS             2362..>2904
                     /locus_tag="AC16V7_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR6255889.1"
                     /translation="MSYEDAIPYVSPVWTRYTPIIAERAEGCYIYDQDGSAYLDFTSG
                     IGVTNTGHCHPRIVEAIRKQAGLLLHGQVNIVYHKPLLELIEELRKITPPSIDGFFFS
                     NSGAEAIEGAMKLARQATGKPNIIVFQGSFHGRTVGTMSLTTSKTIYRAGYQPLMPGV
                     FVAPFPYAYRYGWDEEKTSQW"
BASE COUNT          711 a          691 c          799 g          703 t
ORIGIN      
        1 tcccagccag cgccccgcac ctggatgccg tctatgcctg ggtaaactat attgagcagg
       61 gtgatatgtt ctggatgatg ttgcgcgact tcccctacac tgtgcccaat gctgccgctc
      121 tggaatacgc caaagagaac gagactgagt tgtacgatgc ctatatcaac tcgccgatca
      181 ccaacacgcc tcccgaagta ctggccaatg gcttcttcat ggaagatgtc ggcgaggcca
      241 ccccgctcta cgaccaaatt tggacggagg ttaagggcga ggagtaattc ccaatcgtga
      301 gaaacccgtt ttctccaaga aaacgggttt ctattgcaaa ggaggttgct atggcttatg
      361 gatacaatgg aaagatcctg catgttgatt taactcacgg cacgctggag atagaagagc
      421 cccctgagag cttttaccgg aagtatatgg gcggcagtgc gatggggcta tattacatct
      481 tgagagatat gcccgcaggt gtcgatcctc tatcgccgga caacatactc acattaatgg
      541 ctagtgtgac gacgggagcg gctatttctg ggcagagccg tatcaatgcg aacgcaaaat
      601 ctcctatggg cggagcgatc ggcgattcgc aatcgggtgg ttttttcccc gcggagctca
      661 agtttgctgg ttttgatggc atagtcgtca aaggacgctc agagaaaccg gtttatctca
      721 gcattttatt tggtgaggta gctcttcacg atgcttccca tttggttggt aaggtgaccg
      781 gtgaggtaga tgcgctcctt agggatgaag tgaatgagcc caaggcagaa gttttgcaat
      841 acgggcctgc cgcagaaaac ggcgtgcgct tctccagcct ggtgagcatg gcaaatcgca
      901 acaatgggcg aacaggtatg ggactggtga tggcgagtaa aaacctcaag gccattgtgg
      961 tgcgaggcaa cgccaaggta caaatcgccg acccaaagaa gctagtggaa ctcaaccgca
     1021 gtgggccaaa gttattgcct gaaaatcccg atgtggatgc tctcttccag catggcactg
     1081 ctaatgtgct gatgccacaa aatacgacgg gcacgttgcc aaccttcaac tacaacgaag
     1141 gtcaatttga gttctgcgaa gatataagcg gcgagcgcat gaccgagaca atccttaagg
     1201 atcgtgatac ctgctatgca tgtgtggtgc gctgcaaacg cgtggtggag atcactgagg
     1261 gagcttacaa agcatatccc ctttatggtg gcgcggagta tgaaacccta tccacgttcg
     1321 gctcgtattg tggtgttaag gatttggagg caatttcttt agctaaccaa atctgtaata
     1381 tgtacggagt ggacacgatc tcatgcggtg cgaccattgc tttcgccatg gagtgctatg
     1441 agaaaggcat aatcggatca agtgagactg gtggtatcga attgcggttt ggcaacaccc
     1501 aggctatgct ggatacgctc cagcaaatag ttgaaaacac tggtccactt ggcaaggtgc
     1561 tctcgcaggg ttctgagcgg gcagcaaagg tgtggggtcc agcggctgaa gattgtttga
     1621 ttacagtcaa aggcgaggaa gccccagccc atatgcctca ggctaagaaa tcgcttgcct
     1681 tgatctacgc tgtcaacccc ttcggtgcag atcaccagtc cagcgagcac gaccctatgt
     1741 acgaggatga ttgtttgccg ctttaccttg agcgattggc agagctggat ttgaaggatg
     1801 tgcctcccta tggatcttta ggacgggaaa aggtacgctt cgccacttac acccacatct
     1861 tctacagcct gctggatacc ttggagctgt gccaattcgt ttggggacct gcatggacgc
     1921 tttatgggcc ttcagaaata gttgagttcg tgcgcgcggt gactggttgg aatgtcagtt
     1981 tgttcgaatt ggtgaaagtt ggtcagcgcc gcttgaacat gctgcgtgcg ttcaatgccc
     2041 gcgaaggctt tagcaggaaa gatgacaagc tgcccaagaa gttttataag gcgcttcaag
     2101 gaaccggtcc aacggctggc gtggcgattg atcctgcgga gtttgaagct gccctcgaca
     2161 tgtactacca actcttagct tggactccgg atggcacccc cacccggact gccctggcag
     2221 atttgggtat tgagtgggtg gctgatacag taggtgttta gtagatacaa ctcaagaatg
     2281 agtgtagaga gcattacctg atataatgcg ctctctacac tcatgcctat cattcattta
     2341 gataaaaaag agcatagtct tatgagctac gaagacgcaa ttccttatgt ttcccctgtt
     2401 tggacgagat atacacccat catcgctgag cgtgctgaag ggtgttatat ctacgaccag
     2461 gacgggtccg cttacctgga ctttacatct ggtatcggcg tgaccaatac tggtcactgc
     2521 catccgcgaa tagtcgaagc cattcgcaaa caagctggct tgctgctgca tggtcaggta
     2581 aatatcgtat atcacaagcc gctacttgag ctgatcgaag agctgcgcaa gataacacct
     2641 ccaagcattg atggattctt ctttagtaac tcaggtgcag aggcgattga aggggcgatg
     2701 aagctggcgc gccaggcgac cggcaaacct aatatcatcg tattccaggg tagttttcat
     2761 gggcgcacgg ttggaactat gtcattgacc acctcaaaga ccatctaccg cgcaggttac
     2821 cagccgctca tgccgggtgt ttttgtcgct ccgtttccat atgcgtaccg ctatggctgg
     2881 gatgaagaga agaccagcca gtgg
//
LOCUS       DBNHOH010000100         2627 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_453118, whole genome shotgun sequence.
ACCESSION   DBNHOH010000100 DBNHOH010000000
VERSION     DBNHOH010000100.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_453118"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(394..603)
                     /locus_tag="AC16V7_01860"
     CDS             complement(394..603)
                     /locus_tag="AC16V7_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017308403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HIR6255890.1"
                     /translation="MADTTSFKVVVNHEEQYSIVPADQKLSGTWREVGKRGTQKQCLT
                     YIEEVWTDMTSADYKSLVTRLNIRR"
     gene            complement(881..1909)
                     /locus_tag="AC16V7_01865"
     CDS             complement(881..1909)
                     /locus_tag="AC16V7_01865"
                     /inference="COORDINATES: protein motif:HMM:NF024869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR6255891.1"
                     /translation="MYDILPKVRKWVEDGQTAAMATVIRTWGSSPRQAGAKMAVNQHG
                     EMTGSVSGGCVEGAVVEAALETLQTGQAQYLHFGVADETAWEVGLACGGMIDVFVQPL
                     DPEIFRQQCSAYEKDAASAHLVQVKGTPGTIGRQWFSLLDNAASYFHDSGIPSQVADA
                     ASSAMSSRVIDASTQEGGEVEFFLDIQLPKPLLIIVGGVHISVALISMAASLGYRTVV
                     IDPRRAFGSEARFAHADRLIHEWPDIALERIPISRSTAIAVLTHDPKLDDPALIKALQ
                     SNAFYVGALGSRTTQEKRRERLRDAGITEDQLGRLYGPIGLDLGGRSPEEIALSIMGE
                     IVRVRNSL"
     gene            complement(1920..>2627)
                     /locus_tag="AC16V7_01870"
     CDS             complement(1920..>2627)
                     /locus_tag="AC16V7_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR6255892.1"
                     /translation="SLQLGDRLSRRRKPGKRERLDLRRSMRKSYKHGGEILHWARLDR
                     KTKPRPIIIIADISGSMERYTRLLLHFIYGLAQDRLHPVEAFVFSTRLTRITRQLNNR
                     EIETALRQVSDTVHDWAGGTRIGESLKTFNFDWGRRVLGRGAVVMIISDGWDRGDIDL
                     LETEMDRLQRSCFRTIWLNPRLGSPEYEPLTRGIVAALPYVDDFLPVHNLASLEDLAD
                     HLAKVSSGRSWHTKNLS"
BASE COUNT          571 a          760 c          701 g          595 t
ORIGIN      
        1 atgttctcga tcaacacatg tctggtttgc tccgcaatct gttcccaaag tgagttattg
       61 agaggaacat ctttctcatc aactaagacg tactgaacac ctgaaatggc ttgatcggca
      121 tcttttcccc actgtgaaag aatgtcaagc gcttcatccg aagggaagct ttccagtggc
      181 gcatggagct atttgaactg gatgggaaaa tatgtaccat aaccactagc aactgattgt
      241 ccgtgataca tggaataaaa catttgcgga ccgctcaacg caactgaaag cggcatctgg
      301 aatctcgaag acttttccgg cccatctcgc gccgcccagc tcatctacgg ttgaacctgg
      361 tattcagaag cttatccatc aatgagagga ttcctatcta cggatattca accgcgtcac
      421 caagctttta taatcagcgg acgtcatgtc ggtccatacc tcctcgatat aggtcaagca
      481 ctgcttctga gtcccacgct tgccaacctc gcgccacgtg ccagataatt tttggtctgc
      541 tggcacgatg gaatattgct cttcatgatt tacaacgact ttgaagcttg tggtgtctgc
      601 catatcgcct ccaagtgggt gccaatgttg gattaagtat atccaattga attgttcgaa
      661 aataatattg taagctaaca gacctttgac aaggtcatca tttttgatag gtactgcagc
      721 aacccaccca atgatagaag agattttacc ctccttgcgg gcaagccaac ccctgggtaa
      781 tttgcttcca gtaggagagc tggtacgatt aaaaacctat ccatcaacct acggcttcac
      841 ctccattcga gggaactgcc tcaaggcttg cgctgatctc tcacaggctg ttgcgcaccc
      901 gcacaatctc ccccataatg gagagcgcga tctcctcagg cgacctgccc cccaggtcga
      961 gcccgatggg accgtacagc ctgccaagct gatcttcggt gatacccgca tcccgcaggc
     1021 gttctcgccg tttttcctgc gtggtgcggc tgcccagtgc gcccacgtag aaagcgttgc
     1081 tctgcagggc tttgatcagc gccggatcat ccagcttcgg atcgtgggtc agcacggcga
     1141 tggcagtgga gcggcttatc ggtatccgct caagggcaat gtccggccat tcgtggatca
     1201 ggcggtctgc gtgggcaaaa cgggcctcgc tgccaaatgc ccggcggggg tcaatgacga
     1261 ccgtgcggta gccaagcgag gctgccatgc ttatcagagc gaccgagatg tgcaccccgc
     1321 ccacgatgat caataacggc ttggggagct ggatatcaag gaaaaactct acctctcccc
     1381 cctcctgcgt ggaagcgtca atgactcgcg aggacattgc gctggatgcc gcgtctgcaa
     1441 cctgcgaggg aatgcccgag tcatggaaat acgaggctgc attgtcaagc agggagaacc
     1501 actggcgccc aatagtaccc ggggtaccct tgacctgtac aagatgtgca gaggcggcat
     1561 ccttttcata cgcactgcac tgctggcgga atatctcggg atccagtggc tgcacgaaga
     1621 cgtcgatcat gcccccgcag gccagcccaa cctcccaggc ggtttcgtcg gcaacgccga
     1681 agtgcaggta ctgtgcctga cccgtctgca gggtttctaa ggccgcctcg accactgcgc
     1741 cctccacgca gcccccactg acggatcccg tcatctcccc gtgctggttg accgccatct
     1801 tggcgccagc ctgcctgggc gaggaacccc aggtgcggat aacggtcgcc atggcagcag
     1861 tctgcccatc ttctacccat ttgcgtactt ttggaaggat atcgtacatg cagtgccttt
     1921 caactcaggt tttttgtgtg ccagctcctg cccgaagaga ccttggcgag gtggtcggca
     1981 agatcttcca ggcttgccag gttgtggacg ggcaggaaat cgtccacata gggcaaggca
     2041 gctactatgc ccctggtgag cggttcgtac tctggggatc ctaacctggg attcaaccag
     2101 atggtgcgaa agcagctacg ctgcaatctg tccatctcgg tttcaagcag gtcaatatcg
     2161 ccgcggtccc aaccatcgct gatgatcatc accactgcac cacgccccag cacccgccgc
     2221 ccccaatcga aattaaaggt tttcaatgat tcgccgatgc gcgtgcctcc agcccaatcg
     2281 tggaccgtgt ccgagacctg gcgcagagcg gtttcgatct cgcggttgtt gagctgccgg
     2341 gtaatgcggg ttaaccgggt gctaaagaca aacgcttcta ctgggtggag ccggtcctgg
     2401 gcgaggccgt agatgaagtg gagtagcagg cgggtgtagc gttccatcga accgctgata
     2461 tcagcgataa tgatgatagg acggggtttg gtcttgcgat ccaaccgggc ccagtggagg
     2521 atctcaccgc cgtgtttgta gcttttacgc atcgatcgcc gcaggtccag ccgctctctt
     2581 tttccgggct tccgccgccg gctcaaccga tccccaagct ggaggga
//
LOCUS       DBNHOH010000101         2545 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_68943, whole genome shotgun sequence.
ACCESSION   DBNHOH010000101 DBNHOH010000000
VERSION     DBNHOH010000101.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_68943"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="AC16V7_01875"
     CDS             <1..848
                     /locus_tag="AC16V7_01875"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6255893.1"
                     /translation="LTISPSIVKSIMNQFMDRISGIKIAVDVALEQIDTADYNLIGQV
                     IQQYLDTSATQSDDLAETLLEVQPPDKKQEQEDINPVLRIVGPIMGGMMIFYAFYTGA
                     SSAQSILREEEERTLPRLFTTPTAQATILSGKFLAVFLTVLVQMIVLLIAARLIFRIE
                     WGGLAPVALMTAGVVLSASSFGIFINSMLKNTKQSGAVFGGVLTMTGMIGMISVFAVN
                     SPAATRLGNSVSLMVPQGWAVRALLQSMNGQPVADVLLTTLVMLAWGAVFFIAGVWRF
                     NHRYV"
     gene            858..2051
                     /locus_tag="AC16V7_01880"
     CDS             858..2051
                     /locus_tag="AC16V7_01880"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6255894.1"
                     /translation="MMRIIDIAAKDLLQYMRERNTFLFFLIMPVLLTLLMGYAFGGFG
                     GSDADPRLPVGFLDEDRTWLSREFQSLLITSEVVRLEESSSRPDLEAKVADEKLAAAM
                     IIPPGYSHAIMRGKSAKLILIADTTTPVGSTVESEALTMLIRLESAVRTALIMEQVAA
                     DRSPFDYTFEQSLSAWQQPPISVQDSTSSKIKKKDNGPISTANTSPGMMLQFAIAGLS
                     VSAQVIVNERKTRCLQRLLTTATARLHILLGHYLAIFALIFSQFLLLIAFAQVILKLD
                     YLAEPFATLLVALAAALCIAALGLLIGTLAKTEEQAIIFSIVPMFILAGLGGAWVPLE
                     VTSPAFQAVGHFSPVAWAMDGFKSIVSRGLGLASVLLPAGVLVGYALLFFGLAAWRFQ
                     YSQEN"
     gene            complement(2072..>2545)
                     /locus_tag="AC16V7_01885"
     CDS             complement(2072..>2545)
                     /locus_tag="AC16V7_01885"
                     /inference="COORDINATES: protein motif:HMM:NF019341.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6255895.1"
                     /translation="GQMQEGISLMRQGVAAGRFKEERCYMTETLCALVEAQAMAGQLA
                     EGLATLGEAFSLVEESGERFWEAEHHRLRAELLLMQGDEEGAEASLESAIEVARRQSA
                     KSLELRSTIDLARLWRKQGKPGEARQVLAEIYNWFTEGFDTLDLMEANALLEELS"
BASE COUNT          502 a          774 c          666 g          603 t
ORIGIN      
        1 ccctgaccat cagcccaagt atcgttaaat cgatcatgaa ccagttcatg gatcgcatct
       61 ctggcatcaa aatcgctgtg gatgtggcac tggaacagat tgacacggcc gattacaacc
      121 tgattggtca ggtcatccag caatacctgg atacctcggc aacacaaagc gatgatctgg
      181 ccgaaacatt gctcgaagtg caacccccag ataagaagca agaacaagaa gatataaacc
      241 ctgtgctgcg cattgtcggc cccatcatgg gtgggatgat gatcttctat gctttctata
      301 ctggcgcctc gtccgcccag agtatattga gagaggagga agagcgcacc ctgccgcgcc
      361 tgttcaccac ccccaccgcg caggctacca tcctcagcgg gaagttcctg gcagtgttct
      421 taactgtatt ggttcagatg attgtgctgc tcatcgcggc gcgcctgatc ttccgcatcg
      481 agtggggtgg tctcgcccct gtggcactga tgaccgccgg cgtggtgctt agcgcctcat
      541 cttttggcat ttttatcaac tcgatgctta agaacaccaa acagtccggc gcagtcttcg
      601 gtggcgtttt gaccatgacc ggcatgatcg gcatgatcag cgtattcgcc gtgaattcgc
      661 ctgccgctac caggttaggc aatagcgtct cgctgatggt gccacagggc tgggcggtgc
      721 gggcgctatt acagtcgatg aacgggcagc cggttgccga tgtattgctg accacactgg
      781 taatgctggc ctggggtgcg gtctttttta ttgccggcgt ctggcgcttt aaccaccgtt
      841 atgtatagga tttcgccatg atgcgcatca tcgatatcgc cgcgaaagac ctgctgcaat
      901 atatgcgcga gcgcaatacc ttcttgttct ttctcatcat gcccgtctta ctcaccctgc
      961 taatgggtta tgcttttggc gggtttgggg gaagcgacgc agaccctcgt cttccggttg
     1021 gcttcctgga cgaggataga acttggctca gccgagagtt ccaaagttta ctgatcacat
     1081 cggaggtcgt ccgcctggag gagagcagct cgcgcccaga cctcgaagcc aaggtcgccg
     1141 acgagaaact ggccgccgct atgatcatcc ccccaggcta cagccatgca atcatgcgtg
     1201 gtaagagcgc caagctgatc ttgatcgcgg ataccaccac acccgttggc tcgaccgttg
     1261 agagcgaggc gctgactatg ctgatccgcc tggaaagcgc cgtgcgcact gccctcatca
     1321 tggagcaagt ggcagccgac cgctctccgt ttgattacac cttcgagcag tccctttccg
     1381 cctggcaaca gcctcccatc agcgtccaag actcgaccag ctcaaagatc aaaaaaaagg
     1441 ataacggccc aatctcgaca gcaaatacct ccccgggcat gatgctgcaa ttcgccatcg
     1501 ccgggctttc ggtatcagcg caagtgatcg taaatgaacg caaaacccgc tgcttacaac
     1561 gcttgctcac cactgccaca gcacgccttc atatattgct cggtcactac ctggcaattt
     1621 ttgccctgat cttcagccag tttcttctgc tcattgcctt tgctcaggtc atcctgaagc
     1681 tggactatct ggctgagccg ttcgccactt tgctggtagc cctggctgct gcactatgca
     1741 tcgctgcttt agggttgctc atcggcaccc tggccaaaac cgaggaacag gccatcatct
     1801 tctccatcgt ccccatgttc atcctggctg gattgggcgg ggcatgggtg ccgctagagg
     1861 tcaccagccc cgcattccag gcggttggcc acttctcacc agttgcctgg gctatggatg
     1921 gctttaagag tatcgtgtct cgcgggctgg gtttggcctc ggtgctactc ccggctgggg
     1981 tcttggttgg gtacgcgctg ctatttttcg ggctggctgc ctggcgcttc caatacagcc
     2041 aggagaattg aggctagtta gtggagatgc tttaagacag ctcctcgagc agcgcattgg
     2101 cttccatcaa atccagtgta tcgaaccctt ctgtgaacca gttgtagatc tccgccaaaa
     2161 cctgtcgcgc ctcgcctggt ttgccctgtt ttcgccacag gcgagccagg tcgattgtag
     2221 atcgcagctc caacgacttg gcgctctgcc ggcgggcgac ctcgatcgca ctttccaggc
     2281 tggcttcggc tccctcctca tcaccctgca tgagcagcag ctctgcccgc aagcggtggt
     2341 gctccgcctc ccagaaacgc tcgccactct cttctaccag cgagaatgcc tcgcccaggg
     2401 tagccagccc ttctgctaac tgtcccgcca ttgcctgtgc ctccaccagg gcgcaaagtg
     2461 tttcggtcat gtagcatcgt tcctctttga accgaccagc cgccacgccc tggcgcataa
     2521 gcgatatccc ctcttgcatc tgtcc
//
LOCUS       DBNHOH010000102         1373 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_68948, whole genome shotgun sequence.
ACCESSION   DBNHOH010000102 DBNHOH010000000
VERSION     DBNHOH010000102.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_68948"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1373)
                     /locus_tag="AC16V7_01890"
     CDS             complement(<1..>1373)
                     /locus_tag="AC16V7_01890"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6255896.1"
                     /translation="HWLDDYSKAFISHLERALTVDESRSYPIAIFAAARREGRIPIFD
                     QRVTVQELDLVGFSSQDLARLAEKILKSSPSSQLLGLLEERSQGNPFFAEQILRYLQE
                     EGHLVLDADGCRPVGTGDQPLLPADVRAVLISRLDRLGEEVRNVVQTASILGREFELG
                     LLVRMSQPDERFFQKMAVALEASIWTELSELRYLFLHALLHDTAYQMLVRTHRRELHA
                     LAVEAIEGLYADGLNLHYGELAYHSEQAEMVEKARRYLQLAGDAARDAYQNNQAVDYY
                     SRAFALTPESDLEGRYHLLLEREALYALLGDSQQRGQDLEALARLAEALDEHTERPGR
                     GARGAEVGERSARYAHTLGDYQGAAAQAEKAAELAGVAGVKDVEVRAHLTWAYAMLRQ
                     GKHEAAVLQAEKGLELARQTGDQHEQSRALNFLGLIAWEQSDTETARRYYEHSLEVAR
                     ESGNLRR"
BASE COUNT          284 a          436 c          362 g          291 t
ORIGIN      
        1 atctacgcaa attgccgctc tctcgggcaa cttccaggct gtgctcataa taacggcgcg
       61 ccgtctctgt gtcgctttgt tcccaggcga ttaaacccag aaagttcaac gcccggcttt
      121 gctcatgttg gtcgccggtc tggcgcgcca gctccaagcc cttctcagcc tggagtacag
      181 cggcctcgtg ttttccttgt cgtaacatgg catatgccca ggtaaggtgg gcgcgcacct
      241 ccacatcttt gacgccagca accccggcca attctgctgc cttctcagcc tgtgcagccg
      301 caccctgata atctccgagg gtgtgggcgt atctagccga ccgctctccc acctcggcac
      361 cccgcgcccc tctccctggg cgctcggtgt gttcgtctaa cgcctcggca agcctggcca
      421 gcgcttccaa gtcttgcccg cgctgctggc tatctccgag caaggcatac aacgcttcgc
      481 gctccaacaa cagatggtag cgcccttcaa ggtcactctc aggggtcagg gcaaacgccc
      541 ggctgtaata gtcaactgcc tggttatttt gataggcatc ccgggcggca tcaccggcga
      601 gttgcaggta gcggcgcgcc ttttcaacca tttcagcctg ttcgctgtga taagccagct
      661 cgccataatg caaatttagc ccatcggcat acaggccctc tatcgcctct accgcaaggg
      721 cgtgcaattc ccggcgatgc gttctcacca gcatttggta agcagtgtcg tgcagcaagg
      781 catgcaggaa tagatagcgc agctcgctca gctcagtcca tatagaggct tccaaggcaa
      841 ccgccatctt ctggaaaaat cgctcgtcgg gttgcgacat gcgcaccaga agccccaatt
      901 caaactctcg acctaagatc gaggcggttt ggactacatt tctcacctct tcacccaagc
      961 ggtccagacg agagatcagc acggcgcgca catccgccgg aagcagcggc tgatctcccg
     1021 ttccaactgg gcggcatcca tctgcatcca gaaccaggtg tccctcttcc tggagatagc
     1081 gaaggatctg ctcggcaaag aaagggttgc cctgcgaacg ctcttcgagc aagccgagca
     1141 actgggatga aggggagctt ttcagaatct tctctgccag ccgtgccagg tcttggctgg
     1201 agaatcccac cagatcgagc tcctgaactg tcacacgctg atcaaaaatc ggtatacgcc
     1261 cctcacgccg ggcagcggcg aatatggcga tggggtatga gcggctctca tcaacggtca
     1321 gggcgcgctc caggtgagag ataaaagcct tagaataatc gtccagccag tgt
//
LOCUS       DBNHOH010000103         1623 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_774106, whole genome shotgun sequence.
ACCESSION   DBNHOH010000103 DBNHOH010000000
VERSION     DBNHOH010000103.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_774106"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1623)
                     /locus_tag="AC16V7_01895"
     CDS             complement(<1..>1623)
                     /locus_tag="AC16V7_01895"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF036799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR6255897.1"
                     /translation="MQGPAIQGRVSIEITEPRVNPRFVLGLVENVEIKPSPYQVQRRL
                     RLAGTRPINNIVDATNYAMLEVGEPLHAFDYDVLLKRAGGKAPRIITRLAEPGERLTT
                     LDDVERQMDAFTVMVTDQSGALALAGVMGGAESEVNEGTRNVLLEGAAWNMINTRRTV
                     LAQNLPSEAAYRFSRGVHPAMAERGVRRGLELMRQWSGGVVAQGLVDNYPLPPVAPTI
                     EFSSLEVMRWLGIQLSSAEIEDILMRLEFAVELRGEMIRVTPPDHRLDIGEGVIGLAD
                     VMEEIARIYGYDHIPETRMADALPPQRDNPKLELEERMRDLLVGLGLQEVITYRMTTA
                     EREARLYPDGKLPIDKPAYLRLANPIASDRVVMRQSLLSSLLEIVERNSRLYPRQVLF
                     EIGQVFLPGVEGGMPVEDTRLAIVLTGPRTDTGWQASDASPMDFFDLKGLLAALLDSL
                     HIRNARYEPGGSASFHPGRCARVLVRELELGFLGELHPLVRERYLSGHSPAQGELSDI
                     PLLAAELGLESLYNLVPERYDITPVPAFPPVL"
BASE COUNT          344 a          520 c          460 g          299 t
ORIGIN      
        1 ccagcacagg cgggaaggcc ggcacgggtg taatatcata gcgctctggc accaggttgt
       61 aaaggctctc caaccccaat tctgccgcta atagaggtat atcagacagc tcaccttgag
      121 ccggcgaatg cccgctcaga tatctctcac gcactagagg atgcagctcg ccgagaaagc
      181 ctagttcaag ctccctgacc aaaacacggg cgcagcgccc aggatggaaa gatgcgctac
      241 cacccggctc atatcgcgca tttcgaatat gcaggctgtc cagcaaagca gccagcagac
      301 cttttaaatc gaagaagtcc attgggctgg catccgaagc ctgccagccg gtatcagtgc
      361 gcggcccggt caagacaata gccagcctgg tatcttccac aggcatgccg ccctccaccc
      421 cgggtaagaa cacctgcccg atctcgaaca gcacttggcg gggatacagc cgggaattac
      481 gctcgacgat ctccaacagg ctggatagca ggctctggcg catcacgacg cgatcgctgg
      541 caatcgggtt tgccaggcgg agataagccg gcttgtctat gggcagcttg ccatctgggt
      601 agaggcgcgc ctcacgctcc gcggtggtca tgcggtaagt gatgacctct tgcagaccca
      661 accccaccaa caggtcacgc atgcgctctt ccaactccag cttcgggtta tcgcgctggg
      721 gaggtaaggc atcagccatg cgtgtttcgg gtatgtggtc ataaccgtag atgcgggcga
      781 tctcctccat cacatccgcc aagccgatca ccccctcacc gatatccagc cgatggtcgg
      841 gaggtgtaac gcggatcatc tccccgcgca gctcaacagc gaactccagg cgcatcaaga
      901 tgtcttcgat ctctgcggag ctaagctgga ttcccaacca gcgcatcacc tccagggagg
      961 aaaattcgat ggttggagca actggcggaa gcggataatt atctaccaat ccctgtgcga
     1021 ccacaccccc actccactgg cgcatcagct ccaagccacg gcgcacgcca cgctcagcca
     1081 tcgctggatg cacgccgcgc gaaaatcggt atgccgcctc agacggcagg ttctgcgcca
     1141 gcacggtgcg gcgggtattg atcatattcc aggcagcgcc ttccagcagc acgttgcgcg
     1201 tcccctcgtt gacctccgac tcggcgccac ccatcacgcc tgccagggct aacgccccgg
     1261 actggtcggt caccatcacg gtgaaggcat ccatctggcg ctccacatcg tccagggtgg
     1321 tcaggcgttc accaggttcg gcgaggcggg tgataatgcg cggcgctttg ccaccagccc
     1381 gcttgaggag cacatcgtaa tcgaaagcgt gcagcggctc accgacttcc agcatggcgt
     1441 agttggtggc atctacgata ttgttaatcg ggcgtgtgcc cgccagtcgc agacgacgtt
     1501 gcacctggta ggggctgggc ttaatctcaa cgttttcgac caatcccagg acgaagcgcg
     1561 ggttgacccg cggctcggtg atctcgatgg agacgcgccc ctggatcgca ggcccctgca
     1621 tgg
//
LOCUS       DBNHOH010000104         2645 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1735788, whole genome shotgun sequence.
ACCESSION   DBNHOH010000104 DBNHOH010000000
VERSION     DBNHOH010000104.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1735788"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /locus_tag="AC16V7_01900"
     CDS             <1..1393
                     /locus_tag="AC16V7_01900"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HIR6255898.1"
                     /translation="KISLANLEAENIFGYLRDEMLDLGLDSLIPERFHHQHRENVGTY
                     FSDPVARPMGAQMSLYARRRDGSEFPVDISLNPLKIGEDLFVACAVRDISERKRAEMA
                     LREREETYQALFEHSNDAIFLLSPSRVHLQVNQKAADLLGYTREELIGMSIFDIVVPT
                     EYPEALNRYASLTRGERVPIYERNFRKKDGSTITVEINLSLIRDNDGKPVMIQSIVRD
                     ISERKMIQQKQLEMLEEIKHSRENLRVMAARIENTREEERRDLADKLHDRVGQNLTGI
                     NINLNIISNLVIREDFNAIQSRLEDTIELVEETTRLVRNVMAELHPPLLDDYGLFSAL
                     RWYIDKYSQRTNIQTILLGEECQPRLPAHVELVLFRIVQEALNNITKHAQADQVTVIL
                     NSTSQIATLSVKDNGRGFDAVLLSKSDKEPHWGLLNMQEKAHSIGGRMMITTAPGQGV
                     NISVEVPREVRDD"
     gene            1386..2030
                     /locus_tag="AC16V7_01905"
     CDS             1386..2030
                     /locus_tag="AC16V7_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6255899.1"
                     /translation="MIKVLLADDHTIIREGLKVYLEMQSEIQVVGEATNGSEAVEKAC
                     QLEPDVVVMDISMPELNGIEATKKIIQNCPQTRVIILSMLGTPEHVFKALQAGALGYL
                     LKESASRDVVDAVFSVYAGKRYLSEPITNILVSDYLLIHGSENVQDPLESLSQREREI
                     LLLVVEGKSSAEIGKMLYLSPKTVETYRSRMMKKLGVSDLAGLVKYALQHGLID"
     gene            complement(2187..2552)
                     /locus_tag="AC16V7_01910"
     CDS             complement(2187..2552)
                     /locus_tag="AC16V7_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255900.1"
                     /translation="MPRHDLNRDLSPLQDLRPQQALALQARSGQERWGYKAFNGFYCD
                     IRSGPDQVDAMDKALPNGKEMRERGIVADYLRHPSAPLNVILETLLLSADERLRSGDY
                     AEVELLLQAVNAALDIFAP"
BASE COUNT          724 a          630 c          709 g          582 t
ORIGIN      
        1 caaaatttcc ctggcgaacc tagaggctga gaacatattc ggctacctgc gcgacgagat
       61 gcttgatcta ggtctggatt ctctgatacc ggaacgtttt caccaccagc atagggaaaa
      121 tgtcggaacc tatttttctg atccggttgc caggccgatg ggcgcacaga tgagtcttta
      181 tgcaaggcgg agagacggca gtgaattccc tgttgacatc agcttgaatc cactaaaaat
      241 aggagaagac ctattcgtag cctgcgcggt gcgggatatc agcgagcgaa aacgggcgga
      301 aatggcattg cgagagcgcg aggagaccta ccaggcgtta tttgaacatt ccaatgatgc
      361 aatatttttg ctttctccct caagggtcca cctacaagtt aatcaaaagg ccgccgactt
      421 gttgggctac acccgggaag agctaatcgg gatgagcata ttcgacattg ttgtaccaac
      481 cgagtatcca gaagctttga acaggtatgc cagtttgact aggggagaga gagtgcctat
      541 atatgaacgc aacttccgta aaaaagacgg gagcaccata acggttgaaa tcaacctctc
      601 cctaataaga gataatgatg gcaagccggt catgatacag agcattgtgc gcgacatctc
      661 agagcgaaaa atgatccaac aaaaacagct cgaaatgtta gaagagataa aacattcacg
      721 cgaaaatctg agagtgatgg ctgcacgcat cgaaaacacc cgcgaggaag agcggcgcga
      781 cctagctgat aaattacatg accgtgtcgg ccaaaacttg actgggatca atataaattt
      841 gaatatcatc agcaacctgg tgataaggga agatttcaat gcaattcaaa gtcgcctgga
      901 ggacaccatt gaactggtcg aggaaaccac ccgccttgtg cgcaatgtaa tggctgaact
      961 gcacccgccc ttgctggacg attacgggct gttctcggct ctgcgctggt acatcgataa
     1021 atattcccag cgcacaaata ttcagacgat attattgggt gaggagtgcc aaccccgcct
     1081 tccggcacat gttgagctgg ttttattccg catcgttcag gaggcgttaa ataacatcac
     1141 caaacacgcc caggcagacc aggtcacggt tatattaaat tcgacctctc aaattgcgac
     1201 cttgagcgtc aaagacaatg gcaggggttt cgatgcggtg cttttatcga aatcggataa
     1261 agagccacat tgggggctgc tcaacatgca ggaaaaggcg cattcaattg gaggtcgaat
     1321 gatgatcacc acggctccag gccagggggt gaatatctcg gttgaagtac caagggaagt
     1381 gagagatgat taaagtattg ctggcagacg atcacaccat catcagggag ggattgaagg
     1441 tctacctgga aatgcaatcc gaaatccagg tcgtgggtga ggcgaccaac gggagcgagg
     1501 ccgtcgagaa ggcctgtcaa ttggaaccag atgtcgtggt gatggatatt tcgatgcccg
     1561 aactaaacgg catcgaggca accaaaaaaa taatccagaa ttgtccccag acgcgggtga
     1621 ttattctctc catgttgggg acgccagagc acgttttcaa ggcgttgcaa gcaggcgcgc
     1681 tgggttatct actcaaagaa tcagccagcc gggacgtcgt ggatgcggtc ttttctgtct
     1741 atgcaggcaa acgctatctt tcggaaccga tcaccaatat cctggtatcg gattatctcc
     1801 tgatccatgg ttcggagaac gtgcaagatc cgctggagag cctgagtcaa cgggaacgtg
     1861 aaatccttct gttggttgtg gaagggaaat ccagcgctga gatcggtaaa atgttatacc
     1921 tttcgccaaa aacggtggag acctaccgca gccggatgat gaaaaaatta ggtgtctccg
     1981 atctagcggg gttggtgaaa tacgccctcc aacatggact tatagattaa gaactgttca
     2041 cgagtcgttt gcgctgcttg aagcaaactt gggtggtcta gttcccgttt gctcaaaggg
     2101 tagaaaacca caaagcgctc gacaaatata tcaagcgctt tgtgtgggga gaatgggact
     2161 cgagcacaca gcctactgat tcgaactcag ggagcaaaga tatccagcgc cgcgttcacc
     2221 gcctgcagca gcagctccac ttcggcatag tcccctgagc gcaggcgctc atccgctgag
     2281 agcaggagtg tctccaggat cacgttcagc ggggcggatg ggtgccgcag gtagtctgcc
     2341 acaatccccc gctctcgcat ctctttccca ttgggcagcg ctttgtccat tgcatccacc
     2401 tggtcggggc cagagcggat atcgcagtaa aaaccgttga aagctttgta accccaacgc
     2461 tcttgaccac ttcgggcttg cagcgcaaga gcctgctgtg gccgcaggtc ttgaagtggg
     2521 gagagatctc gatttagatc gtgtcgcggc aaagcttctt acagaaataa gcagcgaagg
     2581 ccgactattt gtggatctga ctcgacaaaa acgcaagtca gatcgagaca gtcaacgcct
     2641 gatac
//
LOCUS       DBNHOH010000105         7053 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_325295, whole genome shotgun sequence.
ACCESSION   DBNHOH010000105 DBNHOH010000000
VERSION     DBNHOH010000105.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7053)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7053)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7053
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_325295"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..965)
                     /locus_tag="AC16V7_01915"
                     /pseudo
     CDS             complement(150..965)
                     /locus_tag="AC16V7_01915"
                     /inference="COORDINATES: protein motif:HMM:NF013752.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1744..3057)
                     /locus_tag="AC16V7_01920"
     CDS             complement(1744..3057)
                     /locus_tag="AC16V7_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255901.1"
                     /translation="MSAIDHTMNILVTLTQGLPIGTNLALLHFLWMLVSGALLPQRGA
                     IFPALQSIGLSEQATRRAWAAFRRGAWQTAVILRLWQEQVEGLPSWQVHRHEGYRAIT
                     VDVTAFWRPALRNCPSQHYHPAAKRALPAVIFGVIGEVGDIGGQRLACPLALERVHPK
                     DPCESRLWQDLLRWARRHLAPEDVAVIDAGVKLTDVQAAGLEHYLLRLATNFTARRNQ
                     PLPYGGKGRKPVYGEKIRPLERSYKGKTIAASSPDRVETWTKDGVAMCAEIWEDLILP
                     GVIPAPQAKTFRVYAIYDPAYPTPWLLATPLHLKAATVKAMYQDRWPVEQIPLSAKHM
                     VGAHRQFVHADESIQRLPELALLAGSILSYLAATAPLAPTGFWDRQPKRTPGRFRRML
                     MGKPFPPSYPLPQQLRKKASSTEQLPKGILAHRRKTSASAPLSVS"
     gene            complement(3125..4093)
                     /locus_tag="AC16V7_01925"
     CDS             complement(3125..4093)
                     /locus_tag="AC16V7_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_114281390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HIR6255902.1"
                     /translation="MRTYKQLTQEQRYQIYALMKMGHNQTEIAEVVSVHKSTVSRELQ
                     RNRGQRGYRPRQAHHLALSRRNKATCRIQPEIWALIEAKLRLDWSPEQISGWLIRKEG
                     ISVSQEWIYQYILADQQAGGDLYRHLRCQKKRRKRYGGYDRRGKLPHRRSIEERPEIV
                     DQRERLGDWEVDTILGKGRRQAIVTLTERKTRLALICKVERRTAQAVQEAITHLLKPW
                     KSRVHTLTADNGKEFAGHQEISHILEADFFFAHPYAAWERGSNENMNGLLRQYFPKGR
                     DFDTISEEEIEVAMFKLNHRPRKCLAFNSPFQEFFKLYFPLTDVAE"
     gene            4346..5065
                     /locus_tag="AC16V7_01930"
     CDS             4346..5065
                     /locus_tag="AC16V7_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255903.1"
                     /translation="MKYITSFIIIAMLTLMPLSSGIFTQSASASATYYTGHPTLTILS
                     VKRDSSVTFYANNLPKHDAFRVRMGLMGTRGVRGTIVDSFETGSGGRKKFTFSIPSKY
                     HGDYRIAIRIESKTGSGYFAYNWFYNNTTGAGTGGKSGRKGYSGYPTMKILSVVRNNS
                     VTVRLRNLPPRDEFKVLMGRYGTRGINGIKVISIDTEKGGNQTMTFSIPSGLRGLRRI
                     AIRIQSKTGSGYYAYNWFYNR"
     gene            complement(5165..5632)
                     /locus_tag="AC16V7_01935"
     CDS             complement(5165..5632)
                     /locus_tag="AC16V7_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR6255904.1"
                     /translation="MISPETLRRYPHFAKLTEAQLRALAMIADEVNYTSGSIILEEGK
                     DAEWLYLLVEGCIDLSYKSEESYHPKATKIFQVGEINPGELFGISALIDPFKYNATAT
                     TSEQSTIVKFNAKSLRTISDLDCTLGYTLMQQIAKAVMERLTHTRIQLAAARD"
     gene            complement(5670..6164)
                     /locus_tag="AC16V7_01940"
     CDS             complement(5670..6164)
                     /locus_tag="AC16V7_01940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HIR6255905.1"
                     /translation="MKTLIVGLGNPILGDDGVGWHVARALLKRLKEGYFDIEQPDIKC
                     LSLGGLSLMENLVGYDHVIIIDAINIGRGHLGSIYKFMLDDLPDPNTGHTSSAHDTSL
                     QSALKMGSSIGLKLPASIQVIAIESQIELNFSEDLSPPVAKAVPLAVELLMNELVKSG
                     TLKG"
     gene            complement(6219..>7053)
                     /locus_tag="AC16V7_01945"
     CDS             complement(6219..>7053)
                     /locus_tag="AC16V7_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HIR6255906.1"
                     /translation="YVDLILGDIYTHRTYSIGTVDENNKVNFYDGKVSVVSPDGERIG
                     KYAPNEYTEWIAEHVEPWTYLKFPYLKKIGWKGFVDGKDSGVYFATPLSRLNAAEGMA
                     TPLAQEEYEKFFTTLGGKPVHQRLATHWARLIELLYAAELWVELATDPKITSNNFHIA
                     PTETPTEGVGIVEAPRGTLTHHYKTDNRGVLTEVNLIVGTTNNYAPISMSIKKAAQSL
                     IKRGNVVTEGMLNMVEMAFRAYDPCFGCATHTLPGQMPLQVSIRDSNGNLVDILKQNC
                     P"
BASE COUNT         1655 a         1844 c         1777 g         1777 t
ORIGIN      
        1 aaccgcctcc ggcctgatgg ggggaggaaa aaggaatgat gatggcggga cagctttcca
       61 aacgcgttca tattgggcgg cagtttacca taaagaaaat aaagagtcaa ataggatttt
      121 tcgaatccag acaaattgag atagtgggat catggatcag cctcgtgccc acttaatgaa
      181 aattgaggtg gcttgccacc caaaagcacg accaattctt ccaggggcaa aatggtaaaa
      241 gcgccagcag cgaaaataat gatcggagaa ctccggaacc ccaattggcg gttcaattcc
      301 tgtcgcaatt tgcgaatgcc acgtgctttc agccgctccg ccacctttga cttggcgatt
      361 tgagccacat tgtaggcgat gagcgtaaaa gtcaagcggg cagccgcaat cacggtattc
      421 cgaaaagtcc acaacgcttc ttcgagatgc caaccttcct tgagttctcg aaaaccgctg
      481 ttctcgatcg tccagcgatt gcgccaaaag gtgtaacctt gccaggcagt gttgaagggg
      541 gcggtcgcca ccagtgccca ctcttcctct ttggtcggct gatcgaccgg atagctctga
      601 atggcaaagc cgctcaaggt gggtgtttcc actcctaagg cctgggcagc ttcaacaaag
      661 ccgtcccagt cattgagctc ctccattttc gctactcgca tctgccgagt ttgcttgtgt
      721 ccagacaggt aacgcacatc cagatgttct tgccaggctt gggggagcac tcgtaacagg
      781 cccatcaact gctcatacat gcggcgctct tccggcaagc gcaccatgac atcgatctgc
      841 cgggcatact tcaggtttgc caggatgaaa ccatccgcgt atagggcatc catcagcagc
      901 cattcaagtg catcgcttcc ggcgatgtct cgcaattcgt ccaccatggc caacacgact
      961 tcgccctctt tgcctttttc cgaggcattc cccgtgcgca aacgccagtt gacccacagc
     1021 ggcctatcct tctgcaaaca aatcagcccc acgacccgca tgtctggccc gatgccactc
     1081 ccatccactg cgtacagctt gctgtggatc aacccccgct tgtacagcac ccctacacag
     1141 tgccgacgat acgcatccag gctctctact ttgatccgtt gcagttcttc gcgcaacaca
     1201 tccggatgat agggccgcga ttgggctgat ttcttctcct tccccttccc ccgacgttga
     1261 ttgaaccctt cccgaatttg aatggctgtg tatcccaatt gcagcaacac cgccgcatct
     1321 tcaaacagcg tattggcagc cgcactcaga ctcatcgctt ccacaaaggg caagatcgcc
     1381 agcgcccgta gaagcttctc atccggtatc ccgcttctct cccgttctac ctcgatcaat
     1441 tccactgcat ccagcacttc taactcactg gccagatgtg ctaacgcatc caatgccccc
     1501 tccctggatg tcaagatggc ttcgtattcc ccgcttcgca gcgcttatcg ggcttgcttt
     1561 gttcgttcca ggagcgatgt tggcgcatca aattgggcga gtgctcggtg gtggttagtc
     1621 gccacctaac acagcgttct aaggaagtgt tgtaacttta gtttccgcta aaagcggatg
     1681 acgaagctac cggaatagac ttccgggagc gcagaacctt acaaactgag cccatttctc
     1741 gcttcaagac accgaaagcg gtgctgaagc gctggttttt cgcctatgag ccaggatacc
     1801 cttgggaagc tgctccgtgg acgacgcttt tttgcgaagt tgctggggca atgggtaaga
     1861 tggcggaaat ggcttgccca tcagcatgcg gcggaagcgg cccggggtgc gtttcggctg
     1921 gcgatcccag aagcccgtcg gagcaagagg cgccgtggct gccaggtaac tcaggatcga
     1981 accggccagc agggctaact ctggcaggcg ctgaatgctt tcgtcggcgt gcacgaactg
     2041 gcgatgagct ccgaccatgt gcttggccga aagcggtatc tgctcgaccg gccagcgatc
     2101 ctggtacata gctttcaccg tagccgcttt cagatggagg ggtgtcgcca gcagccaggg
     2161 tgtgggatag gctggatcgt agatggcata cacccggaaa gtcttggcct gaggtgcggg
     2221 gatcacaccc ggcaggatca ggtcttccca gatctcggca cacatggcaa cgccgtcttt
     2281 tgtccaggtt tctacccgat cgggggagct ggcggcaata gttttgccct tgtagctgcg
     2341 ctccagcggg cggatcttct caccatagac gggtttgcga cctttgccgc catagggtaa
     2401 gggctggttg cggcgagccg tgaagttggt cgccaggcgg agcagataat gctccagccc
     2461 ggctgcctgc acatcggtca gcttgacgcc agcatctatc acggcgacat cttcaggagc
     2521 caaatggcgg cgtgcccaac gcaacaagtc ctgccacaag cggctctcgc aggggtcttt
     2581 gggatgcacc cgttccaatg ccagtggaca ggccaggcgt tgaccgccga tgtcacccac
     2641 ttctccgatc acaccgaaaa tcaccgccgg aagagcccgc ttggcagccg gatggtaatg
     2701 ttggctcggg caattgcgca atgccggtcg ccagaaggcg gtgacatcga cggtgatggc
     2761 ccggtagcct tcgtggcgat gcacttgcca gctgggtaac ccttcgacct gctcctgcca
     2821 caagcgcaag atcacggcgg tttgccaggc gcctcgccgg aaagcagccc aagctcgtcg
     2881 cgtagcctgc tcggacaggc caatcgactg cagcgccgga aagatcgctc cccgctgggg
     2941 cagcaaagca ccgctgacca gcatccatag gaaatgtaaa agtgcgagat tggtcccgat
     3001 ggggaggcct tgggtcaaag tgaccaaaat attcatggta tgatcgatgg cggacaatgg
     3061 gctcacctca tggttggatg tttggcaaaa aacaagctta ccatgtttgg ggcccattgc
     3121 ccctttattc cgctacgtca gttagcggaa agtaaagttt aaagaactct tgaaaaggag
     3181 aattgaaggc gagacacttt cttggacgat ggttgagctt gaacatagcg acttcaatct
     3241 cttcttcgga aatggtatca aagtcccgac ctttcgggaa gtattgtcgc aagagaccat
     3301 tcatattttc gtttgatcca cgctcccaag cggcatacgg atgggcaaag aaaaaatccg
     3361 cctccaaaat atgcgagatc tcctgatgac cagcaaattc cttgccattg tctgcggtaa
     3421 gggtatgcac acgcgatttc caaggcttca gaagatgggt gatcgcttct tgaaccgctt
     3481 gagctgtgcg tcgctccacc ttgcagatca aagccaggcg agttttgcgt tctgtgaggg
     3541 tcacgatcgc ttggcgccga cccttgccca agatggtgtc tacttcccag tcaccaagcc
     3601 gttctcgttg atcgacgatc tcaggacgtt cttcaatgct tctgcgatgg ggcaatttgc
     3661 cacgccgatc atagccacca tagcgtttac gacgtttctt ctggcagcgc agatgacggt
     3721 acagatcacc acccgcttgt tgatcagcca gaatgtattg gtagatccac tcctgactga
     3781 cgctgatccc ttcttttctg atcaaccagc ccgaaatctg ttccgggctc cagtccaggc
     3841 gcagcttggc ttcgatcaac gcccagattt ccggctgaat gcggcacgtg gctttgttgc
     3901 gcctgctcag tgcaagatga tgtgcttgcc tgggtcgata accacgctgg ccacgattgc
     3961 gctgcagttc tcgactaacc gtagatttgt gtacactgac gacttcggcg atttccgttt
     4021 ggttgtgacc cattttcatc aatgcataaa tctggtatct ttgttcctgg gtaagctgtt
     4081 tgtaggttct cataagcgct cctttgattt ggctgatcaa gaagctctta tcctacctca
     4141 gcttacctct ttttcagcct gcaaagttgc acttactact tgaatctacg ctcgcttatg
     4201 taaccatttt ttacactttt ctgataaaat ctaaaataat tgttaagtga ggattaacca
     4261 aattagaata caatcatagt atacaggtgt ttcgttgccg acgaaggttt tttaagttct
     4321 gcatgtttat cataaggaga ttgaaatgaa gtatatcacc tcgttcatca taattgccat
     4381 gttaaccctt atgcccttaa gctctggtat cttcactcaa agcgccagcg cttcggcgac
     4441 ctactatact ggccacccta cactgacaat tctcagcgtt aagcgtgatt cttcggtcac
     4501 attctatgcg aataatctac caaagcacga tgcattcagg gtgcgcatgg ggctcatggg
     4561 cacacgcggt gtccgcggaa cgattgtgga tagctttgag actggcagcg gcggcaggaa
     4621 gaagttcacc tttagtatcc ccagcaaata tcatggggat tatcggattg ctatccgcat
     4681 cgagagcaag accggatccg gttatttcgc ctacaattgg ttctacaaca atacaactgg
     4741 tgctggcact ggtggaaaat ccggtcgcaa aggctattcg ggttacccca caatgaaaat
     4801 cctgtcggtg gtcagaaaca attctgtcac tgtacggttg agaaacctgc ctccccgtga
     4861 tgagttcaaa gtattaatgg gccgctatgg cacgagaggc atcaatggca taaaagtgat
     4921 cagtatcgac acggaaaaag gagggaatca gacgatgaca ttcagcatcc cctccgggct
     4981 gcgtggtttg aggcggattg ctatacgtat ccagagcaag actggatccg gttattacgc
     5041 ctacaattgg ttttataacc gataaaaaag ttacttaaca atcggttaga gggtcatgcc
     5101 gagtagatac agcggcatga ccctggataa ttcgttaaat ctcattcgtt cacaaagata
     5161 acttctaatc tcgcgccgca gctagttgga tgcgtgtatg agttagccgt tccatcacgg
     5221 ctttcgcgat ttgctgcatc aatgtgtagc ctaaggtaca gtccagatcg gatatagtac
     5281 gtagtgactt tgcattaaat ttaacgattg tgctttgttc ggaggtggta gcggtggcat
     5341 tgtatttgaa tgggtcaatc agggcgctta taccgaagag ctcacctgga ttaatttcac
     5401 ccacctgaaa aatctttgta gccttgggat ggtaggattc ctctgatttg taactaaggt
     5461 cgatgcaccc ttcaaccaat aaatataacc actcagcatc tttgccttcc tcaagaatga
     5521 ttgatccaga agtgtaattc acttcatcgg cgatcatagc taatgcccgt agttgagctt
     5581 cggtgagctt ggcgaaatgg ggataacggc ggagagtttc gggggaaatc ataagatata
     5641 tacctccatc tgaattgtaa gtctattgat tatcccttaa gagtacctga tttgactagc
     5701 tcgttcatta gtagctcgac cgcaagtggt acggctttag caactggagg gctgagatct
     5761 tcggaaaagt ttaattcgat ttgcgattca atagcaatta cctgtatcga ggcagggagt
     5821 ttcaagccaa ttgaacttcc cattttgagt gcgctttgga gagaggtatc atgtgcagag
     5881 cttgtatgtc cggtgttagg gtccggtaga tcgtccaaca tgaacttgta aatactcccc
     5941 aggtgtccac gaccaatgtt gatggcatcg atgattatta cgtgatcgta acccactaga
     6001 ttttccatca agcttaagcc gccaagcgat aagcatttga tgtcaggttg ctcaatatcg
     6061 aagtaaccct ccttgagtct cttaaggagt gctctggcga cgtgccatcc caccccatca
     6121 tcccctaaaa tggggttgcc taagcctaca atcagtgttt tcacttgata tcagagttga
     6181 aggttgagat caacaagcga acctcaacct tcaacaattt acgggcaatt ttgcttgagg
     6241 atatccacaa ggttcccatt ggaatctcgg atagagacct gcaatggcat ttgaccgggt
     6301 agtgtgtgcg tcgcgcaacc gaagcaggga tcataagctc ggaaggccat ctccaccatg
     6361 ttgagcatac cttctgttac cacattgcct cttttaatca agctttgggc agcttttttg
     6421 attgacatgc taataggtgc ataattgttc gttgtgccaa caattaagtt cacctcggtt
     6481 agcacaccgc gattatcggt tttatagtga tgcgttagtg tgccgcgcgg cgcctcgaca
     6541 atccctaccc cttcggttgg tgtctcagtg ggagctatgt ggaaattgtt ggaagtgatc
     6601 ttgggatcgg ttgccagctc tacccaaagt tcggcggcgt aaagtaactc gatcaggcga
     6661 gcccaatggg ttgcaagtcg ctggtgaact ggtttgcctc caagtgtagt aaagaatttc
     6721 tcgtattctt cctgcgcaag cggggtggcc attccctcag cagcgttcaa tcgggagagc
     6781 ggcgtagcaa agtaaacacc tgaatcctta ccgtccacaa atcctttcca gcctatcttt
     6841 ttcaggtatg gaaacttgag ataagtccaa ggttctacat gctctgcaat ccactcggtg
     6901 tattcattgg gggcatattt tccaatccgc tcgccatcgg gtgaaaccac gctcactttc
     6961 ccgtcgtaga aattcacttt gttattctca tccaccgttc caatcgaata ggtacggtga
     7021 gtgtagatat cgcctagaat caggtcgaca tac
//
LOCUS       DBNHOH010000106         2648 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_5429, whole genome shotgun sequence.
ACCESSION   DBNHOH010000106 DBNHOH010000000
VERSION     DBNHOH010000106.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_5429"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1790)
                     /gene="ptsP"
                     /locus_tag="AC16V7_01950"
     CDS             complement(<1..1790)
                     /gene="ptsP"
                     /locus_tag="AC16V7_01950"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIR6255907.1"
                     /translation="MQSLRLNILNETGLHARPAATFVKTAKKFQASVRVRNITSGSEW
                     ADAKSVLGVLALGVEYGHEIELEIQGDDEDLAAEALQEAIRSGLGEGIVPPQPVQNEL
                     PQTLRGIPAAPGIAQGTAVLWREATLHIPRFTQTDPAAELERIRIAKQSAQEEIIQLR
                     EKVATEASAEEAAIFEAHLLIADDSSLLGQIETSVNQGVNAEAAWADGVDYFAGQLEK
                     LGDPILQARAMDVRDVGQRVLRHLLGETREAGLDLRQPSLVVARDLTPSQTAGLPKDL
                     VLGFCTAEGGPTSHTAILAKALGLPAVVGLGVDLLDVPDGTPLLVDGTRGEVIVRPDR
                     AQLQAFKALQASRTEQALAEQAVAAEAAITLDGHAVEVVANVGEVEDALRSLEFGAEG
                     IGLLRTEFLYLRRESAPTEEEQVQAYTSILEVMGKRPVVMRTLDVGGDKMLPYLDLGR
                     EANPFLGWRAIRLCLDQPDLFKAQLRAMLRAGVGHDLRIMFPMIATLDELRQAKVLLE
                     QARGEVRSAGQAAATSLQVGMMVEVPAAAMMADLFAREVDFFSIGTNDLTQYTMAAER
                     TNQKVAYLGDGCHPAVLRQIQQVVTAGHATG"
     gene            complement(1795..>2648)
                     /locus_tag="AC16V7_01955"
     CDS             complement(1795..>2648)
                     /locus_tag="AC16V7_01955"
                     /inference="COORDINATES: protein motif:HMM:NF051685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF7916 family protein"
                     /protein_id="HIR6255908.1"
                     /translation="AEGRTVAAEVIATAPPLLDKVSNVELAAGMGADLILLNLYDVTA
                     PQVFGIPMENAPGGIKIFGRIPSGQGATIGVVKSFVGRPVGLNLEPIENPEAVTGSGR
                     LANADNARIAVEQGADFILVTGNPHTGVTNRGIRRALAEIRAAVGDRVVLLAGKMHAA
                     GVSGPPVSDAEIRAFADEGANGIVLPAPATVPGITVEIGHRLAEIAHERGLLVMNTIG
                     TSQEGASRSTIEQLALWSKMTGADIHHIGDAGTIGIAVPENIYTWSLALRGRRHTWHR
                     MAASLRR"
BASE COUNT          536 a          859 c          711 g          542 t
ORIGIN      
        1 ccggttgcgt gaccggcggt gaccacttgt tgaatctggc gcagcaccgc cgggtggcat
       61 ccatccccta aatacgctac cttttgattg gtgcgctcag ctgccatcgt gtattgggtg
      121 aggtcgttgg tgccaatgct gaaaaaatcc acttcccggg cgaataaatc tgccatcatg
      181 gccgccgccg ggacttccac catcataccg acctggagtg aggttgcggc tgcctgacca
      241 gcagaccgca cttcgccgcg cgcctgctcc aacaacactt ttgcctgacg cagttcgtct
      301 aatgtagcaa tcatgggaaa catgatccgc aaatcatgac ccacgccagc gcgcagcata
      361 gcccgcagct gggctttgaa caggtcgggc tggtccaaac acaagcggat cgctcgccag
      421 ccgaggaacg ggttggcttc acggcccagg tccagatagg gcagcatctt gtcgcccccc
      481 acgtccaagg tgcgcatgac caccgggcgc ttacccataa cctccaggat actggtatag
      541 gcctgcacct gctcttcttc ggttggtgcg ctctcccgcc tcagatagag aaattctgtg
      601 cgcagcagac cgatgccctc cgcgccgaac tcaagggacc gcagcgcatc ctcaacttcc
      661 cccacatttg ccaccacctc gacggcatgc ccatccaggg taatcgccgc ttccgcggcc
      721 acggcttgct cagccaatgc ttgttccgtg cgactggcct gcaaggcttt aaacgcctgg
      781 agttgggcgc gatcggggcg cacaatcacc tcgccgcgcg tgccatccac cagcaggggg
      841 gtgccatcgg gcacatccag gagatcaact cccagaccaa caaccgccgg cagacccagg
      901 gctttggcca ggatagccgt atgagacgta ggcccaccct ctgcagtaca gaaacccagc
      961 accaggtctt ttggcagccc agcagtctgc gacggggtca gatcgcgagc aacgaccagg
     1021 gaaggttgtc tcaaatccag cccagcttcg cgcgtctctc ccagcaggtg tctcaagacc
     1081 cgctgaccca catcgcgcac atccatggcg cgcgcctgca aaatgggatc accaagcttc
     1141 tccagctgac cggcaaagta atccacccca tccgcccagg cggcctcggc gtttacccct
     1201 tgattaaccg acgtttcaat ttgcccaagc agggagctat catcggcgat gagcagatgc
     1261 gcctcgaaga tagcggcctc ttccgcactg gcttccgtgg caactttttc tcttaattga
     1321 atgatttctt cctgcgctga ttgcttagca attcgaattc tctccaactc ggccgcgggg
     1381 tctgtttggg taaatctagg gatgtgcagg gttgcctcac gccataaaac cgccgtaccc
     1441 tgggcaatgc caggggcagc aggaataccg cgcaatgttt gcggaagttc attctgtacc
     1501 ggctgaggag gcacgatccc ctcacccagg ccagatcgaa ttgcctcttg aagtgcctct
     1561 gccgccaggt cttcatcgtc cccctggatc tccagctcaa tctcatgccc gtattctacc
     1621 cccagagcca agacgcccag cacgctctta gcatccgccc actcgcttcc gctggtgatg
     1681 ttgcgcaccc ggacactggc ctgaaatttt ttcgccgtct tgacaaaggt ggcggcaggg
     1741 cgagcgtgca agcccgtctc attgaggatg tttaatcgca aagactgcat tggattatct
     1801 gcgcaaagaa gctgccatgc ggtgccaggt gtggcgtcgt ccccgtagag ccaaggacca
     1861 ggtgtagata ttctccggaa cagcgatgcc tattgtgcct gcgtcgccaa tatggtggat
     1921 atctgcaccc gtcattttcg accacagcgc cagttgctca atagtcgagc ggctggctcc
     1981 ttcctgggat gtcccaatgg tgttcatcac cagcaagcca cgttcatggg cgatttcagc
     2041 taaacgatgc ccgatctcca ccgtgatgcc aggcacagta gccggagcag gcaagacgat
     2101 cccattcgct ccttcgtcgg cgaaggcgcg gatttccgca tcacttacgg gtggcccaga
     2161 cacccccgca gcatgcattt tccctgccag gagcacaacc cgatcaccca ccgcggcgcg
     2221 tatttctgcc agggcgcggc gaatgccgcg gtttgtcacg ccggtgtggg gattgccggt
     2281 gaccaggatg aagtctgcgc cttgctccac agctatgcgg gcgttgtccg cgttagccag
     2341 ccgccccgag cccgtcacgg cctctgggtt ctcgatgggt tccaggttca agccaactgg
     2401 gcgtccaaca aatgatttga ccacacctat tgtagcgccc tggcccgagg gaatgcgacc
     2461 aaaaattttg atccctccag gggcattctc catgggaata ccgaaaacct ggggtgcggt
     2521 tacatcgtac aagtttagca agatcaagtc agcccccata cccgctgcca gctcgacgtt
     2581 ggagacttta tccagcaacg gcggggcggt ggcaatcacc tcagcagcca ctgtgcgccc
     2641 ctctgcct
//
LOCUS       DBNHOH010000107         6345 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1159048, whole genome shotgun sequence.
ACCESSION   DBNHOH010000107 DBNHOH010000000
VERSION     DBNHOH010000107.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6345)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6345)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6345
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1159048"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="AC16V7_01960"
     CDS             <1..571
                     /locus_tag="AC16V7_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR6255909.1"
                     /translation="VARFREKPDEARARRMLKQGDHAWNSGMFVWRVDRVLSEFATQM
                     PKLYSGLQEIQQAWGSSQQQTVIYRIWDQLVSETIDYGIMEGAKDVAVIMADDLRWND
                     VGSWDSLFDVLPSDPQGNIVMGGEHIGLDTRETLVYVNQEHRLIVTIGVQDLVLVDTG
                     DVLLVCRKDQAQKVRQIVKSLKESGRSYI"
     gene            604..2934
                     /gene="topA"
                     /locus_tag="AC16V7_01965"
     CDS             604..2934
                     /gene="topA"
                     /locus_tag="AC16V7_01965"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIR6255910.1"
                     /translation="MEVIILEAYCVKCKTKRELSDPQPVFTKGGTPATRGVCPVCGTN
                     MVRMGRTPAHEGLTPDETLVKPRQKRSGKLVIVESPAKARTVGRFLGKGYTVKASIGH
                     VRDLLKSQLSVDVDNDFTPKYRVPKEKRQVVNEIKDLVKKAEKIYLATDPDREGEAIA
                     WHLMESARIDPEITQRVVFHEITEPAVAEAFSHPRGINMDLVNAQQARRVLDRLVGYN
                     LSPLLWRKVRSRLSAGRVQSVALRLVVEREREIDAFVPEEYWTIAGEFLPEGGKETFI
                     AKLAKIDKQEPVLSKEADVQPVLAEMQSAVYTTSKVKRGERRRSPAAPYTTSTLQQEA
                     SRRLGYTARRTMAIAQQLYEGVDVGEGGSVGLITYMRTDSTSVSELARSEARNFIAGR
                     FGETFLPKEAPRYKTKSRGAQEAHEAIRPTAVNRQPNSIKDYLTRDQFRLYQLIWQRF
                     LASQMAAAIFDTLTVEITGLAQHEYLLRASGSTVRFPGFLVVYKEARDEDIKTEEEGE
                     TRLPSNLVEGQSQRLVRLLPEQHFTQPPPRFTEATLVRTLEEYGIGRPSTYAPILSTL
                     QSRGYVLRESKRLSPTETGNLVNNMITEYFPNIVDVGFTARMEEDLDRIASGVQPWVD
                     PIKQFYTPFSRQVADADARIPEMKKELEPVGRDCPESGHALVIRYGRYGKFISCSNFP
                     DCRYTEPWLEKIGVQCPQDGGEIVERKTRKGRVFYGCANYPNCDFTSWKKPLPTPCPN
                     CQGLLVVADRRNAQCLNCAEQYPLDQISADELVESA"
     gene            3086..4096
                     /locus_tag="AC16V7_01970"
     CDS             3086..4096
                     /locus_tag="AC16V7_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255911.1"
                     /translation="MDAIREQIKKPLVLGIAGLVVGIALGILYGWVINPVEWTDAAVE
                     HLRADLQQEYLRMSIDSYAQNRDAELAKKRYADLGPNAAEVMGAVMESPGAQNFETIL
                     AFSATVQGGVEPAIPPEGEAAQPEEEKQSSNILRMAAVVLFLIFVVIAGGAFYLFIWR
                     PRMGESVPVTPAMQAQEAAQQAEWTDYSAEGGETPIVQFMSSYKFGDDLFDESFAIDS
                     PAGDFMGECGVGISETIGVGEPKKVTTFEVWLFDKNDIQTVTKVLMSANAFLDEPTRQ
                     RLSAKGEPILAEPGIETVLETETLILVARVVDMGYGEGAVPDSSFFDRLLLELAVWPK
                     ES"
     gene            complement(4197..4664)
                     /gene="greA"
                     /locus_tag="AC16V7_01975"
     CDS             complement(4197..4664)
                     /gene="greA"
                     /locus_tag="AC16V7_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIR6255912.1"
                     /translation="MDDQIYLTSEGAARLKIELEQLKVTGRDEIAQRLRSAVQMGDLS
                     ENADYHKAKEDQGFLEGRIQELEYLLKNATIIEKNGARPEVADIGTHVTIQEGSEPPE
                     TYHLVGAKEADPRNGRISNESPIGRALLGHRAGDEVVVETPNGKIHLRIITIE"
     gene            complement(4710..5861)
                     /locus_tag="AC16V7_01980"
     CDS             complement(4710..5861)
                     /locus_tag="AC16V7_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HIR6255913.1"
                     /translation="MFYTRQHLEALEDQTLAPYACRSKDSKGRAYPEDEPEYRTAFQR
                     DRDRILHTTAFRRLEYKTQVFINYEGDYYRTRLTHTLEVSQIGRTIARTLGANEDLLE
                     AICLAHDLGHPPFGHSGETILARLMQEHGGFDHNKQSLRIITQLEGRYPDFPGLNLTW
                     EVREGIVKHESEYDTADASEYNPDLRGHLEAQIANVADELAYTTHDLDDGLRSGMITP
                     HMLEGITLWEILAESVGWSSPVLDDLTRHRLIRRLIGLEVTDLVNATHARLQQSNVKS
                     VKDLQSLSYNVISFSEDMHRRNRQLKDFLYNHLYRHHRVVRMAVKAERIISDLFHAYC
                     SEPAILPGHIQEFIEERGLERTICDYIAGMTDRFAMDEHQKLFEPSTKP"
     gene            complement(5935..>6345)
                     /locus_tag="AC16V7_01985"
     CDS             complement(5935..>6345)
                     /locus_tag="AC16V7_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid kinase family protein"
                     /protein_id="HIR6255914.1"
                     /translation="IPVVYGDVIFDTARGGTILSTEALFEHLARHLGPERVLLAGLEE
                     GVWADFPACTQLVHEITPESLPQLIPVLGGSNATDVTGGMADKVQQSLALVQELPGLE
                     VLIFSGEKQHQVERALLGERVGTLVRANIPQETI"
BASE COUNT         1442 a         1600 c         1830 g         1473 t
ORIGIN      
        1 cgtagcacgc tttcgtgaaa aaccggatga agcacgggcg cgtcgcatgt tgaaacaggg
       61 tgatcatgct tggaactctg gcatgttcgt gtggcgagta gatcgcgtgc tgtctgaatt
      121 tgccacacag atgccaaaac tatattctgg gttgcaagag atccagcagg cgtggggttc
      181 gtcacagcag cagacggtta tttaccgaat ttgggatcaa ctggtatcag agaccattga
      241 ttatggcatc atggagggcg ccaaagatgt agcagtcatc atggctgatg acctgcgatg
      301 gaatgacgtg ggaagctggg actcactctt cgacgtgcta ccttccgatc cacaaggtaa
      361 catcgtgatg ggcggcgagc atatcggttt ggatactcgc gaaacgcttg tctatgtaaa
      421 tcaggaacac cgcctaattg tgaccattgg tgtccaggat ctggtactgg tggacaccgg
      481 tgatgtgttg ctggtttgcc gaaaggatca agctcagaag gtgcggcaga tcgtaaagtc
      541 gttaaaagag agcgggcgta gctacatctg aggcctgtgt aattggtaag agatagcact
      601 tatatggagg taatcatttt ggaagcttat tgtgttaaat gtaaaaccaa gcgcgaattg
      661 agcgatcccc aacccgtttt taccaagggg gggacaccgg ccacgcgtgg cgtctgcccg
      721 gtgtgcggca cgaatatggt gcgcatgggg cgcacacctg cccacgaggg actgacaccc
      781 gacgagacgc ttgtcaagcc caggcagaaa cgctctggca aattggtcat tgtcgagtcg
      841 ccagccaagg cacgtaccgt gggtcgcttc ctgggcaagg gctacacggt caaggcgtcc
      901 atagggcatg tgcgggattt gctcaaatcg cagctttcgg tggatgtaga caacgacttt
      961 acccccaaat accgtgtgcc caaggaaaaa cgccaggtgg taaacgagat caaagatctg
     1021 gttaagaaag cggagaagat atatctggcg accgaccccg acagagaggg tgaggcaatc
     1081 gcctggcacc tgatggagtc ggctaggatt gaccctgaga tcacccaacg cgtggtgttt
     1141 cacgagatta ccgaaccggc ggttgccgaa gctttttctc acccgcgcgg catcaacatg
     1201 gatttggtga acgctcagca agcgcgccgg gtattggatc gattggtagg ctataacctc
     1261 agtccgctgc tgtggcgcaa ggtgcgcagc cggctctccg ccggtcgtgt ccagtccgta
     1321 gcgctgcgcc tggttgtcga gcgagagcgt gagatcgatg ccttcgtccc tgaggaatat
     1381 tggaccatcg cgggcgagtt tctcccggag ggcggtaaag aaacctttat cgccaagctg
     1441 gctaagatcg ataaacagga gccagtgctg agcaaggaag ccgacgtgca gcccgtcctg
     1501 gcggaaatgc agtcggctgt ctacactacc agcaaggtca agcgtggcga gcggcggcgc
     1561 agcccggcgg cgccgtatac taccagcaca ctgcagcagg aggcttcccg cagattaggt
     1621 tatacagcta ggcgcacaat ggcgatcgct caacagctct acgaaggcgt ggatgtgggt
     1681 gaggggggct cagtcggcct gatcacctat atgcgcaccg attcgaccag tgtttcggaa
     1741 ctggcgcggt cggaggcacg caatttcatc gctgggcgct ttggcgaaac tttcctgcct
     1801 aaggaggctc cgcgctacaa aaccaaatcg cgcggtgcac aggaagccca tgaggcaatc
     1861 cgccctaccg cagtgaaccg acagccaaat tcgataaagg actacctcac tcgtgatcaa
     1921 ttccgtctat accagctgat ctggcagcgc ttcctggcct cccaaatggc agcggctatt
     1981 ttcgatacgc tcaccgtgga aatcaccggc ctggcgcagc acgagtatct gctgcgcgcc
     2041 tccggttcca cagtgcgttt cccgggtttc ctggttgtct ataaagaagc ccgggacgaa
     2101 gacataaaga ccgaagaaga aggtgaaacg cgcctcccat ccaacctagt agaaggtcag
     2161 tctcagcgcc tagtgcgatt gttgccagaa cagcacttca cccaaccccc accgcgcttt
     2221 accgaggcaa cgctggtccg cacactggag gaatatggta tcgggcgacc ttcgacttac
     2281 gcaccgatct tatctacctt gcaaagtcgt ggctatgtct tgcgcgagag caagcgcctt
     2341 agccccacag agacgggcaa cctggtcaat aatatgatca ctgaatactt ccctaatatt
     2401 gtcgacgtag ggtttacggc gcgcatggag gaggatctgg atcgcatcgc ctccggagtg
     2461 caaccctggg tggatccgat taagcagttt tacacgccat tctcccggca ggtggctgac
     2521 gccgatgccc gcattccaga gatgaagaag gaactggagc cagttggaag ggactgtcca
     2581 gaatccgggc acgccttggt gattcgctac gggcgctatg gcaagttcat cagctgcagt
     2641 aattttccgg attgccgtta taccgagcca tggctggaga agattggggt gcaatgccca
     2701 caggatggag gcgagattgt agagcgaaag actcgcaagg gtcgcgtatt ctatgggtgt
     2761 gccaattacc cgaactgcga cttcacttca tggaaaaaac cattaccaac tccgtgccca
     2821 aattgccagg gtttgttagt agtcgccgat cggcgtaatg cccagtgcct gaactgcgcc
     2881 gagcaatacc cgttagacca gatttctgca gacgagttgg ttgagtcggc ataagacttg
     2941 caacaaaccg gatgagcttc tccacttatg aaagctcatg atagaatatg tgtgctaaac
     3001 attattcgca gaacgttgca taaatcaagg gtttcgtcta gaattagaga caacgaatcc
     3061 ggaatcatgg tatttggaga gagatatgga cgccattcgt gagcagatca aaaagcctct
     3121 cgtcttgggc attgctggct tggttgtggg cattgccttg ggtatcttgt acggctgggt
     3181 gatcaacccg gtggagtgga cggatgccgc tgttgagcac ttacgcgccg acctgcagca
     3241 agaatacctg cgcatgtcta tcgattcata tgcccaaaat cgcgatgcag agctggcaaa
     3301 aaagcgctac gcggacctgg gacccaatgc agctgaggtg atgggtgccg tgatggaatc
     3361 cccgggcgcg cagaatttcg agaccatcct ggctttcagt gcgacggttc aaggcggggt
     3421 tgagcctgca attccaccag agggcgaggc tgcacagcct gaagaagaga agcaaagcag
     3481 caatatcctc agaatggcgg cagtcgtcct attcttgata tttgtcgtga ttgctggtgg
     3541 ggctttttat ctattcatct ggcgtccgag aatgggcgaa agcgtccccg tcacacctgc
     3601 tatgcaggcg caggaagccg cccagcaagc cgagtggact gattattcag ctgagggtgg
     3661 cgagacgccc atcgtgcagt ttatgtcatc ttataagttc ggggatgatc tcttcgatga
     3721 atcctttgcc atcgattcgc ccgctggaga ttttatgggc gagtgtggtg ttggtatctc
     3781 agagaccatc ggtgtgggtg agccgaagaa ggtaaccacc ttcgaggttt ggttgttcga
     3841 taagaatgat atccagacgg ttaccaaggt gctgatgagc gccaatgcct ttttggatga
     3901 acccacccgc cagcgcctgt cagccaaagg cgaaccaatt ttggctgagc cagggatcga
     3961 aacggtgctg gaaaccgaga cattgatact agtggcacgc gttgtagata tgggatatgg
     4021 tgaaggcgct gtgcctgata gcagcttctt tgaccgatta ttgctggagt tagcggtctg
     4081 gcctaaagag agttaaaaca tcaggttcag cggtaggtat aatatctcgc tgaacccttt
     4141 atgcacattt gacattgggt tttacgggcg ctacgcaccc gtaaaaccca atgtttttat
     4201 tcgattgtta taatgcgcaa gtgaattttg ccatttggcg tttctacgac tacttcgtcg
     4261 ccagcacgat gccccaggag agccctacca atgggggatt cgtttgagat gcgaccgttg
     4321 cgcggatcgg cctctttggc acctaccagg tggtaagtct caggaggttc agaaccctct
     4381 tggatggtca cgtgcgtgcc gatatccgcc acctcaggtc gggcgccatt tttttcgata
     4441 atcgtggcat tttttaacag gtactctaac tcctggatgc gcccttccag gaagccctgg
     4501 tcctctttgg ctttgtgata atctgcattc tcggagagat cgcccatttg gaccgcggag
     4561 cgcaggcgtt gagctatctc gtcacgacct gtcactttta gctgctccag ttcgattttg
     4621 aggcgagctg ccccttcgga ggtaaggtaa atttgatcat ccatgcttta accttctact
     4681 ttcaagtatc acttaacatc ctaccgatat taaggtttcg tggacggttc gaatagcttt
     4741 tgatgctcgt ccatggcgaa gcggtcggtc atgccggcaa tgtaatcgca gatggtgcgt
     4801 tccaatcctc gctcctcaat gaactcctgg atgtgacctg gcaggatggc aggttcgctg
     4861 cagtaggcat ggaacaagtc gctgatgatc cgctcagcct tcaccgccat gcgcaccacc
     4921 ctgtgatgcc ggtataggtg attatacagg aagtctttga gctggcggtt gcgccggtgc
     4981 atatcttcgc tgaagctaat gacattatat gataggcttt gcagatcttt gaccgatttg
     5041 acgttgctct gttgcaggcg tgcgtgcgtg gcgttgacaa gatcggtcac ttctaagcca
     5101 atcaagcgcc ggatcaggcg atggcgcgtc aggtcatcga gaacggggga actccagccc
     5161 acgctctcag ctaagatctc ccaaagtgtg atgccttcca gcatatgggg ggtgatcatg
     5221 ccggagcgta acccatcatc caggtcgtgc gtggtatagg ctagctcgtc agctacattg
     5281 gcgatctgtg cctccaggtg gccgcgcaga tctgggttgt actcgctggc atcggcagtg
     5341 tcgtattcag actcgtgctt gacaatgccc tcacgcactt cccaggtcag gttgaggcct
     5401 ggaaagtcag ggtagcgccc ttccagttgg gtgatgatac gcagggactg cttgttgtgg
     5461 tcaaaaccac catgctcttg cattaaccga gctaagatag tctctcccga gtgaccaaaa
     5521 ggcgggtgcc ctagatcatg ggctaagcaa atcgcctcta agaggtcctc gttagcaccc
     5581 aaggtgcgag cgatggtgcg cccgatttgg gacacctcca gagtgtgcgt cagacgcgtg
     5641 cggtaatagt cgccctcata attgatgaaa acttgggtct tatattctag gcgacggaaa
     5701 gccgtggtgt gcaaaatacg gtcccggtcg cgctggaatg ccgtgcgata ttccggttca
     5761 tcttccgggt aggcgcggcc ttttgagtcc ttgctgcggc atgcataagg tgccagagtt
     5821 tggtcttcta gggcttcgag gtgctggcga gtatagaaca ttgggattag atcgcagggt
     5881 ttagaacgta aagaatagat aattggccat tcacgccaag tgtgtaatta taatctatat
     5941 tgtttcttgg gggatgttcg cccgcacgag cgtgcccact cgctcgccca gcaaggctct
     6001 ttcaacctga tgttgtttct cgccagagaa aatgaggact tccagtccag gtaactcttg
     6061 aaccaatgct aggctctgct gtaccttgtc ggccatgccc ccagtgacat cggtagcgtt
     6121 tgaaccaccc agtacgggta ttagctgggg caggctttcg ggtgtgattt cgtggactag
     6181 ttgtgtgcag gcggggaaat cagcccacac cccctcttct aatccagcga gcaagacgcg
     6241 ttcaggaccc agatggcggg ccaggtgctc gaagagggcc tcggtcgaga gaatggtgcc
     6301 gccgcgggca gtatcgaata tcacgtcgcc atacacgaca gggat
//
LOCUS       DBNHOH010000108         2013 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_69424, whole genome shotgun sequence.
ACCESSION   DBNHOH010000108 DBNHOH010000000
VERSION     DBNHOH010000108.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_69424"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="AC16V7_01990"
     CDS             complement(<1..1151)
                     /locus_tag="AC16V7_01990"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR6255915.1"
                     /translation="MNENLQPSTIKIPMSSPDLSEAEREAVASVLRTPNLSMGGEITA
                     FEDAVRNYTGRKHAIGVSSGTAGLHLCVRAAGIGEGDLVITTPFSFVASSNVLLYERA
                     VPVYVDVDPMTGNLDPQQVRQAAADLTQGGEAAKKWLPRKAGQDSSRLKAILSVDVFG
                     QPADMDAITETARDYGLKIIQDSCEALGAEYKGRPVGIQGDYGIFAFYPNKQITTGEG
                     GLIITDDDQAAAMMRALRNQGRAPGDTWLRHTYLGYNYRLDEMSAALGRVQMGRLDEL
                     LGKRQRVADWYAERLAEIPAVEPPLIVSATTRVSWFVYVVRLSANIDRDTVAQDLGMR
                     GVPARPYFLPIHLQPYMVERFGYQPGDYPVTEDLGRRSLALPFSGVMSEA"
     gene            complement(1264..>2013)
                     /locus_tag="AC16V7_01995"
     CDS             complement(1264..>2013)
                     /locus_tag="AC16V7_01995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HIR6255916.1"
                     /translation="ALARERGLDLSSLPVGPLITESALRTYLQAEPQPASAFDPHAII
                     VYGGGGHGKSLVDLLRALGTYRVVGFVDDGLPKGQLIMGVPVLGGSEVLPEIHAQGVH
                     LAANAVGGIGDVTVRVKVFHHLAQAGFVCPALSHPTAVIEPSATLTAGVHVFAHAYVG
                     SEARLGYGVIVNTGAIVSHDCLLGDYVNISPGAMLAGGVQIGSGALIGMGVTVNLGVN
                     IGAGARVGNGATVKADVPEKGVVRAGAVWPD"
BASE COUNT          430 a          656 c          539 g          388 t
ORIGIN      
        1 gcttcgctca tcacccccga aaagggtaac gccaggctgc gacgccccaa atcttcagta
       61 actgggtagt caccgggctg gtaaccgaag cgctcgacca tgtacggctg cagatggata
      121 ggcaagaagt acgggcgcgc tggcactcca cgcatgccca agtcctgtgc cactgtatcc
      181 cggtcaatat ttgcagatag gcgcaccacg tagacaaacc agctgacgcg cgtggtagca
      241 gatacaatca ggggtggctc cacagcagga atctccgcca ggcgctctgc gtaccaatct
      301 gccacccgct ggcgcttgcc cagcaattca tccagcctgc ccatctgcac gcgtccaaga
      361 gcggcgctca tctcgtccag tcgatagtta tatcccaggt aggtgtgccg cagccaggta
      421 tcccctgggg ctcggccctg gttgcgcagg gcgcgcatca tggcggctgc ctggtcatcg
      481 tcggtgataa tcaaaccgcc ttccccagtg gtaatctgct tattggggta gaaagcaaag
      541 atgccataat ccccttgtat gccaaccggg cgacctttat attcagcgcc cagggcttcg
      601 caggaatcct ggatgatctt caggccataa tcgcgcgccg tttcagtaat cgcatccata
      661 tccgcaggct gaccgaaaac atccaccgag agaatcgcct tcaacctgga gctatcttga
      721 ccggctttgc gcggcagcca tttctttgcc gcctcgcccc cctgtgtcag gtcagcggca
      781 gcctgcctga cctgctgagg atccaggttg ccggtcatgg gatccacatc cacatagacc
      841 ggcacggctc gctcgtagag cagcacgtta gaagaagcta cgaaggagaa aggggtggtg
      901 atcacaaggt cgccctcgcc aatgccggct gcgcgcacgc acaggtgcag cccggcggtg
      961 cccgagctga ccccaatggc atgcttcctg cctgtgtagt tacgcactgc atcctcgaag
     1021 gctgtgattt cgccgcccat gctcaaattg ggcgtgcgca gaacggaggc tacagcttcc
     1081 cgctctgcct cgctcaggtc tggggacgac atcggaatct tgatggttga aggttgaagg
     1141 ttttcattca tctcttggtg cccaatgatg gatattcacc gatattggtt aagctcttcg
     1201 ttgcaaaaac gtcccgcagg tttatgatca cacaggtttg tttaaagaga acctgcggga
     1261 tgtttaatcc ggccaaaccg cccctgcccg caccacgcct ttttccggca catccgcctt
     1321 gaccgtggca ccgttgccaa cccgcgcccc tgccccaata ttaacgccca aattgacagt
     1381 tacccccata ccgatcaaag ctccgctgcc aatctggacc cctcctgcca gcatcgcccc
     1441 cggggagata ttgacgtaat cgcccaggag gcaatcgtga gagacaatcg ctccggtgtt
     1501 gacaatcacg ccatatccca ggcgcgcctc gctgcccaca taggcatggg cgaatacatg
     1561 cacacctgca gtgagggtcg cactgggttc aatcacggca gtcggatgag agagcgccgg
     1621 acagacgaaa ccggcctgcg ccaggtgatg gaagaccttc acccgcaccg tcacatcccc
     1681 gataccaccc acggcgttgg cagccaggtg tacaccctgg gcgtgtattt ccggtaatac
     1741 ctccgaacca cccagcaccg gcacgcccat aatcaactga cctttcggca agccatcgtc
     1801 cacgaagccc acgacccggt aggtgcccag cgcccgcaac aagtcaacca gcgattttcc
     1861 atgcccacca ccgccataca caatgatcgc atgcggatcg aaggcgctgg caggctgggg
     1921 ctccgcctgc agataggtgc gcagcgcgct ctcggtgatc agcgggccaa ccggcaggct
     1981 gctcagatcc aggccacgct ctcgagccag tgc
//
LOCUS       DBNHOH010000109         5194 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_325554, whole genome shotgun sequence.
ACCESSION   DBNHOH010000109 DBNHOH010000000
VERSION     DBNHOH010000109.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5194)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5194)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5194
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_325554"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..2592)
                     /locus_tag="AC16V7_02000"
     CDS             complement(205..2592)
                     /locus_tag="AC16V7_02000"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR6255917.1"
                     /translation="MIRPRWRKVIRDIWENKVRTLLVVASIFIGIFSVGLTAAMYIMT
                     GEDLNTSWIATNPAHAYIYTSTYQPEFLERVRRLPGVAGAEGRRGVTTRLKIGPDEWI
                     PLFLATKPTYGHSQIDVLKPEAGAWPPGDKELVIERSSLRWANAEIGDTLEIKLIDDT
                     TRKIPLVGVVHDAQAGPGSALGISGYVTFDTLEWLHTERNLSSIAITVSENALDQDHI
                     QAVADGVAERVRESGGEVYYVYVPPPGKHPGYDSVLGVIAIIGALGFMSLFLAGFLIF
                     NTFSALMVQHVRYIGMMKAIGARRSQIVGMYLVLIMAFSLFALLPAIPLSAYAASWVA
                     VMMSQQFNYESLGFRMVPAVVLLQVIVGLVIPLLAGILPVYTGTRITIHKALSSYGLG
                     NSEIRRSVVDRLVENIRGLSRPLLISLRNTVRRKGRLVLTMATLALGGAIFISVFNLR
                     ESLVVFVEQIGKYYLADANVSLQQPYRLQELEQYIMQIPGVKRVEGWAGANAELLRAD
                     GVGTDNVSLIAPPVDSDLIEPVMLRGRWLTPGDQNAIVVSNAFWNTLPDLKVGDEIKL
                     DINDKETTWQVVGFFKFPGDSGLLAYTSYEYLTDLLHSPNRAYSVRIVGEDHSPAAQK
                     KLAKQVEQVLKSRGIQLVGQIQTSSMMTEANAQVINIIVGFFLFFAILVAVVGAIGLM
                     GTMSMNVLERTREIGVMRAIGATNGAVFRIVLVEGMLIGFISWVLGVLAATPITNLLY
                     DILSRAIFQTEGVAVMTWDGFIIWLVMAVVLSAIACLIPARNAARMTVRDVLAYE"
     gene            complement(2589..3623)
                     /locus_tag="AC16V7_02005"
     CDS             complement(2589..3623)
                     /locus_tag="AC16V7_02005"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6255918.1"
                     /translation="MVLKEIDASIYEGEFVSVIGRSGSGKSTMLNMIAGIDRPTSGDV
                     QVGDVSVHELSESDMAAWRGRNLGIVFQFFQLLPMLSLIENVMLPMDFCNVYTPRQRK
                     ERAMELLRKVELDRHARKLPSAMSGGEQQRVAIARALANDPPIILADEPTGNLDSKTA
                     DVVFRLFKDLVDQGKTVVMVTHDSSQAQRATRTMLLVDGEIMNEYVARALPTLTPEQL
                     VKATRSLQPMYFEAGATILQEGMPGENFYIITQGQAEVTLRRPEGTDVVVARLGEGQY
                     FGEIELMQGRKNAASVRASEHSPVELVALNQAAIDSILDASDPTRAVLEKAAADHIAE
                     NIEARREEQA"
     gene            4139..4576
                     /locus_tag="AC16V7_02010"
     CDS             4139..4576
                     /locus_tag="AC16V7_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098015 - virus tail [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HIR6255919.1"
                     /translation="MTPTAGRHDPYRNFNFIVEIDGIASSGFLMVDGLESITEVVDYR
                     EGNEKATPRKLTGISKASNITLKRGVTDNRDLWEWRKTVLDGTPQRRNGSVVILNESR
                     EQVLRVNFQNAWPCRWKIGGLDALDSQVLIEEIELVVEDIRLD"
     gene            complement(4680..>5194)
                     /locus_tag="AC16V7_02015"
     CDS             complement(4680..>5194)
                     /locus_tag="AC16V7_02015"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6255920.1"
                     /translation="LSPMQINDLVALIVAWREGEKVSSNVPMATFVTNALIAIREFDR
                     PDSAFYLKATLPLATGEQAQEIEAILTMIDENRLFEAQTRLVALLPAEEMGKASFSGN
                     CAPCHADNGTGGIGPNLHDNSFVQSKSDEEIVDFIQNGRRGTAMVGFEGILGVEEIDN
                     LIALLREWQK"
BASE COUNT         1250 a         1484 c         1275 g         1185 t
ORIGIN      
        1 tgccgttcca caaaaaacta gcctagcgac ccgatggcac attcgcctga tgagcaaaaa
       61 acggatgggt actcctgctc acatattata tattcaacat aaaatataac acttaagtat
      121 gcgttatgta tatattaaat gacttgcgca atttatgtgt ttgaacgaga tttcggggaa
      181 tcagcaatat tagcggcagg gcggttattc gtacgccaag acgtctcgca ccgtcatgcg
      241 cgccgcattg cgggccggta tcaggcaggc gatggcggac aggaccacag ccatcaccag
      301 ccagatgatg aaaccatccc aggtcatcac cgccacgcct tccgtctgga atatcgcccg
      361 gctcaggatg tcgtacagca aattcgtgat gggggtagcc gccaatacgc ccaaaaccca
      421 gctgataaac ccgatcaaca tcccctcaac aagcacaatg cgaaaaactg cgccattggt
      481 ggcgccaatc gctcgcatca cgccgatctc gcgggtgcgc tccagcacat tcatgctcat
      541 ggtgcccatc agaccaatcg cccccaccac ggccaccagg atggcgaaga acaagaaaaa
      601 acccacaata atattgatca cctgggcgtt ggcctccgtc atcatgctac tggtctggat
      661 ctggccaacc aactggatgc cgcggctttt caggacctgt tcaacttgct tagccagttt
      721 tttctgggct gcgggggagt ggtcttcgcc aacgatacgg acggagtatg cccggtttgg
      781 agagtgcaat aagtctgtca ggtattcata gctggtatag gcgagcagcc ccgagtcacc
      841 cggaaactta aagaagccca ccacctgcca ggttgtctct ttgtcattga tgtcgagctt
      901 gatctcatct cccaccttga ggtctggcaa cgtattccag aaagcgttgc tcaccacgat
      961 ggcgttttga tcgccggggg tcagccagcg gccgcgcagc atcaccggct cgatcagatc
     1021 gctatccacg ggcggagcga tgagggacac gttatcggtg ccgacgccat ccgcgcgcag
     1081 caattcagcg tttgcgcccg cccagccttc cacgcgcttc acgccgggaa tttgcattat
     1141 gtactgctca agttcctgta gtcggtaagg ttgttgcaag ctgacgttgg catctgccaa
     1201 gtagtacttg ccgatctgct cgacaaaaac caccaaagac tctcgcagat tgaaaaccga
     1261 tataaaaata gcacctccga gcgccagtgt agccatggtg agcaccaggc gacccttgcg
     1321 ccgcaccgtg ttgcgcagag agatcaggag cgggcgcgaa agtccgcgaa tattttctac
     1381 cagccggtcc acaacgctgc ggcgaatctc gctattcccc agcccatagc tgctgagcgc
     1441 cttgtggatg gtgatgcgcg tgccggtata cacaggcaga atccctgcca gcaacggtat
     1501 taccaatcca acaataactt gcaagagaac taccgctgga accatgcgaa aaccaagcga
     1561 ttcgtagttg aactgctggc tcatcataac cgccacccat gaagctgcgt aggcagaaag
     1621 cggtatggcg gggagcaagg caaataagct gaaggccatg atgagcacca gatacatgcc
     1681 cacaatttgt gagcggcgcg ccccaatggc tttcatcatg ccaatatagc gcacgtgctg
     1741 caccatcaat gccgaaaatg tgttaaagat caagaaaccc gccaggaata gcgacataaa
     1801 gcccagcgcg ccaatgatcg cgatcacccc cagcactgag tcatacccgg gatgctttcc
     1861 cggtggcggg acataaacat agtacacctc tcccccgctc tcacgcacgc gctcggctac
     1921 accatcggca actgcctgga tatgatcctg gtccagcgca ttttcggaga cggtgatggc
     1981 gatgctagat aggtttcttt cggtgtgcag ccactccagc gtatcaaagg tgacatagcc
     2041 gctgatcccg agcgcggaac ccggacccgc ctgggcatcg tgcacgacac ccaccagcgg
     2101 gattttgcga gtggtatcat cgatgagctt gatctccagc gtatcaccaa tctctgcatt
     2161 agcccaccgc aagctgcttc gctcaattac cagttcctta tcgcctgggg gccaggcgcc
     2221 ggcttctggt ttaagcacgt cgatctggga atgaccatag gtgggttttg tcgccaggaa
     2281 gagaggtatc cactcatccg gtccaatctt caagcgagtg gtgacccccc gcctgccctc
     2341 agcccccgcc acaccaggca agcggcgcac cctctccaaa aactcgggct gatatgtgct
     2401 ggtatagata tatgcatgag ccgggttcgt cgctatccag ctggtattca aatcttcacc
     2461 ggtcataata tacatggcgg cggttagacc cacgctgaaa ataccgataa agatcgatgc
     2521 cacaaccagc aacgtgcgca ctttgttttc ccatatatca cggatgactt tgcgccagcg
     2581 tgggcggatc atgcttgctc ctccctgcgc gcttcgatgt tttccgcaat gtggtccgcc
     2641 gcggctttct ccagcacagc acgtgttggg tctgacgcat ccagaatcga atcgatggca
     2701 gcttgattca gggcaaccaa ttcaaccggg gaatgttcgg aagcccttac gctggccgcg
     2761 tttttcctgc cttgcatcaa ttcgatctca ccgaagtatt gcccctcacc caggcgagcg
     2821 acaaccacat cggtgccttc cggtcgtcgc aacgtcacct cggcctgccc ctgtgtgata
     2881 atgtaaaaat tctctcccgg catcccctct tgcaagatgg tcgcgccagc ctcaaagtac
     2941 atgggctgga ggctgcgggt ggctttgacc aactgttcgg gggttagtgt aggcaatgcc
     3001 ctggctacat attcattcat gatctcgccg tccaccaata acatggtacg ggtagcccgc
     3061 tgtgcctggc tgctatcatg cgtcaccatg acgactgttt ttccctgatc gacgagatct
     3121 ttaaagagac gaaacaccac atctgccgtt ttggagtcca ggttgccggt tggctcgtct
     3181 gccaggatga tcggggggtc gttggctagc gcacgggcaa ttgccacgcg ttgctgctca
     3241 cctccagaca tcgcagaagg gagttttctg gcatgccggt ctaattccac cttgcgaagc
     3301 aactccatag cccgctcttt gcgctgccgc ggggtataca cattgcaaaa atccatgggc
     3361 aacatcacat tctctatcaa ggagagcatc ggcaataatt ggaaaaattg gaagacgatg
     3421 cctaaattgc gacctcgcca ggcagccata tcgctttcgc tcaattcgtg gacggacaca
     3481 tcgcccactt gcacatctcc agaggtaggg cggtcgattc cggcgatcat gttcagcatg
     3541 gtcgattttc cgctgccgga cctgccaatt acagatacga actcaccctc atagatgctg
     3601 gcatcgatct ctttcaatac catgaattcg cccgccgtgc tggaatatgt tttaacgagt
     3661 ctgtccagct tgatgattgg ctttttacca ttgcccccaa tatgattaag gttaatgcga
     3721 tcaacctttt ttcgttgaaa aaccctggaa aacattactt cctccccgga atacagctac
     3781 tcctttagaa tttaaaatca aataggtcta cagctcaacg tcctatctat atgtatatac
     3841 taaaatttcc aagaaaagtt tcagaaccct ggccatcatg ggctattcaa acatcgctca
     3901 tgtggggaga ttaccctccg tgacataaat ttaaggcacg aattttaggg cgcaatcaat
     3961 aggcactcgg ctgatataat acccgcattg aataagccgc gagctcaagt gtttcttcgc
     4021 cgacaccatc gtatgggtat cccaacggga cattactctc tgcacgtatt cggaggattg
     4081 gatgccacca tctagccagc ccaagcgcaa accccgcaag ttgaaaaagg aggaaaaaat
     4141 gacccccact gcaggtcgtc atgacccata tcgaaatttc aattttattg tcgagattga
     4201 tgggatcgca tcctcaggtt tcctgatggt ggacggtctc gaatcgatta ccgaggtggt
     4261 ggattaccgc gaaggcaacg aaaaggctac gccgcgcaag ctcaccggaa tctctaaggc
     4321 ttccaatatc accttgaagc gcggtgtaac cgacaaccgg gacttgtggg aatggcgcaa
     4381 aaccgtgctg gatgggaccc cacagcgccg taatggatct gttgtcatcc tgaatgaatc
     4441 gcgcgagcag gtcctgcgtg tcaacttcca aaacgcctgg ccttgccgct ggaaaatcgg
     4501 cggtttagat gccctggatt cccaggtgct gatcgaggaa atcgagctcg tcgtggagga
     4561 tatcaggctc gactagaacg cccttcaggc ccgcgaatcg attactccgg ttaaacgcca
     4621 actccataac tcaccatcac accatgttac gcatgacggt gcgttcctgc ctgattgttc
     4681 tatttttgcc actcacgtag caaagcaatc aaattgtcga tctcttcaac ccccaggatg
     4741 ccttcaaagc caaccattgc cgtccctctg cgaccattct gtataaaatc aactatctct
     4801 tcatcgcttt tcgattgcac aaatgaattg tcgtgaaggt ttggcccaat cccccctgtt
     4861 ccattgtcag catggcatgg ggcacaatta ccgctgaaag acgctttccc catctcttca
     4921 gcaggcagga gagcgactaa acgagtttgc gcctcaaaca gacggttttc gtctatcatc
     4981 gtgaggatag cttcgatctc ttgagcctgc tcgccggtgg ccagcggcag ggttgccttc
     5041 aggtaaaacg ccgagtccgg acggtcgaat tcacggatcg cgattagggc attggtaaca
     5101 aaagtcgcca tgggcacatt tgagctcact ttttccccct cacgccatgc tactattaga
     5161 gcaaccagat cgtttatttg catcggtgaa agca
//
LOCUS       DBNHOH010000110         1837 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1095235, whole genome shotgun sequence.
ACCESSION   DBNHOH010000110 DBNHOH010000000
VERSION     DBNHOH010000110.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1095235"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="AC16V7_02020"
     CDS             complement(<1..278)
                     /locus_tag="AC16V7_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HIR6255921.1"
                     /translation="MNSIIECIPNFSEARRPEVVAAIRQAIAAVPDAFVLDRHSDLDH
                     NRSVITFAGSLEAVEEAAFRAIAKAAELIDLDEHTGEHPRIGATDVVP"
     gene            583..>1837
                     /locus_tag="AC16V7_02025"
     CDS             583..>1837
                     /locus_tag="AC16V7_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR6255922.1"
                     /translation="MSPRPARFRLGALPLVFVVLLGALLACARTAQSDEPPALWMPGQ
                     NAPQLQESGQATRTPFMPPTRLPGAPILTPTPDEPHSLPAMRTEPDQYFVQPGDTLGI
                     IAQRFGVSLEDLVKANELINPNLLDVGQMIIVPVPTPQGLGPDFKIIPDSELVYGPAS
                     ALFNIEAFIKSQGGYLAGYREEVEGKQLTGDQIVQRVAMEYSVNPRILLAALQYQSSW
                     LTKTDLSQGKIDFAMGIREEWRKGLYRQLSWAANNLNRGYYLWRANAAATWLLGDGSV
                     VPVASTINAGTAGVQHMFAALYGRADWEQAVGENGVFSVYNTFFGYPFDYAIEPLLSP
                     GLRQPPMQLPFEPEVAWAFTGGPHGGWGDGSAWAALDFAPPSDLLGCIVSNDWVTAVA
                     DGLILRAENGAVIQDLDGDGYEQTGW"
BASE COUNT          366 a          511 c          539 g          421 t
ORIGIN      
        1 aagggcacca catctgtggc accaatgcgc gggtgctcgc cggtgtgctc atccaggtcg
       61 atcagctcag cagctttggc gatggcgcgg aacgcggctt cctccactgc ctccagggag
      121 ccagcgaagg tgattacaga gcgattatgg tccaggtcgg aatgccggtc taagacaaaa
      181 gcatccggca cggccgcgat agcctggcga atggcggcga ctacttccgg tcgccgcgct
      241 tcagagaagt tagggataca ttcgatgatg gaattcatag ggaggagaga attaagagta
      301 gttgatagta agctgggagc agtttgttaa ctgctgtttg cccagaaagt gccctgctaa
      361 ttatactcaa taacagaagt ggcgcagttg ggtagtgact tgggtgattt ggtagttttt
      421 gatgcgccca aatccccctc tttaccttcg gcagcgcagt tctatcaatg aatgtctggc
      481 aggactcttg cacaaaatgg gtataatgca cagcgtgtgc tacacttcga acttgcaacc
      541 cggtagctct gatatgttag aatgtcctcc cagagacgct tcatgagccc tcgtcctgct
      601 cgctttcgcc tcggggcttt gcccctggtc tttgtcgtcc tgctgggtgc gctgctggcc
      661 tgtgctcgca cggcacagtc tgatgaacct cctgccttat ggatgcccgg gcagaacgcc
      721 cctcaactcc aggaatccgg gcaggcgaca cgcacaccct ttatgccccc cacacgcctc
      781 cccggcgcgc ccattctcac cccaaccccc gacgagcctc acagcctgcc agccatgcgc
      841 accgagccag accaatactt tgtccagccc ggcgacacgt tgggcatcat cgcccagcgt
      901 ttcggggtca gcttggagga tttggtgaag gcgaacgagc tgataaaccc caatctcctg
      961 gatgtcggtc agatgattat tgtccccgtg ccgacacccc agggtctcgg cccggatttt
     1021 aagattatcc ctgattcaga gctggtctac gggccagcca gtgctttgtt taacattgag
     1081 gctttcatca agagccaggg ggggtatctg gcgggttacc gcgaggaagt cgaggggaag
     1141 cagcttaccg gagaccagat tgtccaacgc gtcgctatgg aatactcggt gaatccccgc
     1201 atcctgctgg ctgcgctgca atatcaaagc agctggctga ctaagaccga cctgagccag
     1261 ggaaaaatcg atttcgccat ggggatccgc gaggagtggc gaaagggcct ctaccgccag
     1321 ctttcttggg cagccaacaa tcttaatcgc ggctattacc tttggcgggc gaatgccgct
     1381 gccacctggt tgttgggcga tggcagtgtg gtgcctgtcg catccaccat caatgctggc
     1441 accgctggcg tgcagcatat gttcgccgct ttgtacggtc gcgccgactg ggagcaggcc
     1501 gtgggtgaga atggtgtgtt ctctgtctat aacacctttt ttggctaccc ctttgattac
     1561 gccattgagc cattgttgtc gcctggattg agacaaccgc ccatgcagct gcccttcgag
     1621 ccagaggtag cttgggcttt caccggagga ccacatggtg gctggggtga tggatcagcc
     1681 tgggctgccc tggattttgc tccgccttcc gacctgctgg ggtgcatcgt aagcaatgac
     1741 tgggtgacgg ctgtcgccga tggattgatc ttgcgtgctg aaaatggggc tgtcatccag
     1801 gacttggacg gggatggcta tgaacaaact ggctggg
//
LOCUS       DBNHOH010000111         1372 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1543504, whole genome shotgun sequence.
ACCESSION   DBNHOH010000111 DBNHOH010000000
VERSION     DBNHOH010000111.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1543504"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="AC16V7_02030"
     CDS             <1..469
                     /locus_tag="AC16V7_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255923.1"
                     /translation="YEPWEYTPTVAAKYYARGIMMHLGMDLWAGIGGENYDQIPPIVK
                     VVQNLSASMAGAKPAELAVEIQSEAANIMSFGFSLPDGEMLLALWTNGAAIEDDPGVS
                     ATLTFPGLSAQKVVGIDVLNGFEQEMLIDNQDDNLLINNLLVKDYPILLRLIP"
     gene            657..>1372
                     /locus_tag="AC16V7_02035"
     CDS             657..>1372
                     /locus_tag="AC16V7_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6255924.1"
                     /translation="MMESKWWLRHYDEGVPHTLQPYPESTLLDIVKETSTQRPEHTAL
                     LFKGSKITYAELDRLSAAFANALIDLGVKKGDRVALMLPNSPQFIISLLGVWKAGGVV
                     VPLNPLYTEHELQHALADCGAETIMVLTVFYSKVKAIQKQLNIRNIIATSIKEYLPPV
                     SRVLFTVLREKKDGHRITLQAGDFWFPDLIRKFSNAPQPNVQIQPDELAILLFSGGTT
                     GTPKAAMGTHKALLISGTQIR"
BASE COUNT          349 a          338 c          346 g          339 t
ORIGIN      
        1 ctatgagcct tgggaatata ctccaactgt agctgccaag tactatgctc gaggcatcat
       61 gatgcacctg ggaatggatc tttgggcagg gattggtggg gagaattatg accaaatccc
      121 gcctatcgtg aaggtagtcc aaaatcttag tgccagcatg gctggagcca aacccgcaga
      181 attagccgtc gaaatccaaa gtgaggcagc caacatcatg agctttggtt tctccctgcc
      241 cgatggtgag atgctgttag ccctctggac caatggcgcg gcaatcgaag acgaccctgg
      301 cgtgagcgca accctcactt tccctgggct gtctgctcaa aaagtggttg ggatagacgt
      361 gctaaatggc ttcgagcagg agatgctcat agacaaccag gacgacaacc tgctcatcaa
      421 taatttgttg gttaaggact atcctatcct ccttcgcttg atcccctaga ttctgtttgc
      481 attagggttc ttatctatcg tttggtgagt atgcgtgttg catatatcga actggcaagt
      541 caaatacctg ttatccgctg caggaggtga gtattgaata tagtgaacaa cgcttcaatt
      601 tggtgagcat gagctaagcg catattctgc aactgtccgg atgccagagg aggtgcatga
      661 tggagagtaa atggtggctt cggcactatg atgagggggt tccacacaca ttgcaacctt
      721 atcccgaatc gacattgctt gacattgtca aagaaactag cacacaacgt cccgagcata
      781 ctgcgttact atttaagggc tctaaaatta catatgctga actggatcgc ttgagcgctg
      841 ccttcgcaaa tgccctgata gacttgggcg taaagaaagg cgatcgtgtc gcactgatgc
      901 tgcccaattc accccaattc attatctccc tgctcggtgt atggaaagcg ggaggtgttg
      961 tcgtcccatt gaatccgctc tacacggaac atgagctgca gcatgcctta gcagattgcg
     1021 gcgctgagac catcatggtg cttacggtgt tttacagcaa agtcaaagcc atccagaagc
     1081 aactcaatat ccgtaatatc atcgccacaa gcatcaaaga gtacctgccg cctgtatccc
     1141 gcgtattgtt cactgtttta agagagaaga aagacggcca tcggataacg cttcaggcag
     1201 gcgatttttg gtttccagac ttgatccgga aattctctaa cgcgccgcaa ccgaatgtcc
     1261 agatacagcc ggatgagctg gctattctgc ttttctccgg gggtacaacc ggcacaccca
     1321 aagcggcgat gggcacacac aaggcgcttc tgatttccgg cacgcagatt cg
//
LOCUS       DBNHOH010000112         4449 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1287278, whole genome shotgun sequence.
ACCESSION   DBNHOH010000112 DBNHOH010000000
VERSION     DBNHOH010000112.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1287278"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="AC16V7_02040"
     CDS             complement(<1..360)
                     /locus_tag="AC16V7_02040"
                     /inference="COORDINATES: protein motif:HMM:NF021384.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HIR6255925.1"
                     /translation="MADAREKLHTNSQTLKLLVFVAVGFLFLGWLFNTPPGLLGKADA
                     IGYAVCHRIDLRSFHLGDRTLPLCARCTGMHLGALLGLVYQSAFSRRKAGLPPIRVLV
                     VLGLLASAFFLDGGNSFL"
     gene            520..1722
                     /locus_tag="AC16V7_02045"
     CDS             520..1722
                     /locus_tag="AC16V7_02045"
                     /inference="COORDINATES: protein motif:HMM:NF022702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HIR6255926.1"
                     /translation="MMNKRWTLVGLMVVALSACSTFAAPAPTPTTEATSTPSLAPSET
                     PTVTPTVQPTPTATETPSPPTATPLPQDVGPANFPQGVNPLTGLTLADPALLERRPLA
                     VKIQMFPRGQRPPWGLSLADIVYDYYQNDGMTRLHALFYGNDAEIVGPIRSARLLDNR
                     LVRMYRSIFAFGGADQRILNVLLNSEYYDRLIVQGSSSPMWREDPNGANYLLTGIQQM
                     AEYINSKGISNSRQNLDGMRFELQPPAGGGSASQVFVRYSISAYNRWDYDPTTGSYLR
                     YVDTQEDTGQGESYEQLVDRLTEQPISAQNVVVVFAVHDDYLRNGRYNVVDILLDVSG
                     PAFIFRDGQAYEVRWYRPQLESVLSLLDLNGNPFPFKQGNTWFQVIGQSSRWGNESGV
                     WRFESRIP"
     gene            1754..2995
                     /locus_tag="AC16V7_02050"
     CDS             1754..2995
                     /locus_tag="AC16V7_02050"
                     /inference="COORDINATES: protein motif:HMM:NF022702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HIR6255927.1"
                     /translation="MKTGLSSIAVLWALMISGCAAVALDPQVSVSSTPPAEVSEIATE
                     EPSAALEVIATIPIATETATPSVTLEPPTTATSFVYAYGPSDFPEPINPLTGLPVDPP
                     QLLERRPIVIKVTNFPRSVRPQWGLSQADHVYEYYIGDSMSRFVGVFYGKDAERVGPI
                     RSARLFDAHVMRMYHGIFIFGWADDAVLKFLFAPDLKFHLIVEQAENCPPLCRFGLKE
                     AYNTLYADTAQIAAYLTKRRTNNDRQNLSGLRFELETPASGNGGELFSIQYSLVSYHR
                     WEYDPLAGVYLRYQETGAKNLSDSGYAPLMDSLTERQISATNVIVMLVPHQYFKKSNS
                     TEIIDQPFMGQGAGYAFRDGQIYPLIWRRQAADQLPELLLPNDRLYPLKPGNTWFEII
                     GETTQFEQLEDGSWEFVSSIP"
     gene            complement(3043..4059)
                     /locus_tag="AC16V7_02055"
     CDS             complement(3043..4059)
                     /locus_tag="AC16V7_02055"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HIR6255928.1"
                     /translation="MYNLFRPLLFTLEPEQAHNLSLAAMRMVGRFAPLRAAMRNLFAA
                     AEEPVHAFGLTFRNPVGLAAGYDKDGLAWRGLACLGFGHIEVGTVTPRPQDGNPRPRL
                     FRLPEDRALINRMGFPGRGADFVRQQLTGERPPGLILGVNLGVNKDTQLERAADDYLV
                     LIDIFAPLADYLVINVSSPNTLGLRRLQARQALETLLRALTTRRKEISRATGNYIPLL
                     VKLAPDLNSGELDDALEAILHFNLDGVIAANTTVSRDRLRSPLAVETGGLSGAPLRKL
                     STQMIRQIAKYTGGKLAIVGAGGVMSASDARQMLDAGAVLVQVYTGLVYEGPSLVKKI
                     IAAI"
     gene            complement(4135..>4449)
                     /locus_tag="AC16V7_02060"
     CDS             complement(4135..>4449)
                     /locus_tag="AC16V7_02060"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0046132 - pyrimidine ribonucleoside
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIR6255929.1"
                     /translation="GTRAEIEGVYSQGERVVVIDDLATTGGSKFEAIEKLTHAGLQVQ
                     DVVVLIDRQSGAGEALNRAGYRMHAIYTLLELINLWEGSKSITGEQARIVREFLGTPD
                     GN"
BASE COUNT         1004 a         1213 c         1201 g         1031 t
ORIGIN      
        1 gagaaatgag ttgccgccgt ctaagaaaaa agcgcttgcc agcaaaccca ggacaaccag
       61 gacacgaatt gggggtaagc ctgctttgcg gcggctgaag gcgctctgat aaaccaggcc
      121 gagtaaggcg cccaggtgca tgccggtgca gcgcgcgcac aatggtagcg tgcggtctcc
      181 tagatggaag gagcgcaggt ctatgcggtg gcacaccgca taaccgattg cgtctgcttt
      241 gcccagtaag ccagggggag tgttgaaaag ccagcctagg aataggaacc ctacggcaac
      301 gaaaaccagc aatttcaaag tctggctatt tgtgtgcagt ttttcccgtg catcagccat
      361 acgagttgaa ttatagagtg tttcaagaaa aacgcaagtt ttgcggtatg attacccagg
      421 gttttcaaaa ggatgggatt tggggtcctc tttgggcttt cacaaggttg aaagccctgt
      481 atgattacct tttgagtttt tttctggaga atggagatta tgatgaacaa gcgatggact
      541 ctggttggtt tgatggttgt ggctctgagc gcatgttcaa cctttgccgc gcctgcgccc
      601 acgcccacta cagaggcgac cagcaccccg agtctagcac cgagcgaaac acccacagta
      661 acaccaactg tacaacccac ccctactgcc acagaaacgc cttcgccacc caccgccacc
      721 ccactcccgc aagacgttgg cccagcgaat ttcccgcaag gtgttaatcc tctcaccggg
      781 ctaactcttg ctgatcctgc cttgctggag cgccgtccgc tggcggtgaa gatacagatg
      841 ttccctcgcg gccagcgccc accctggggt ctctcgctgg ctgatattgt ctacgattat
      901 tatcaaaacg atggcatgac ccgcctgcat gccctctttt atggaaacga cgctgaaatt
      961 gtcggcccga ttcgctctgc gcgcttgttg gataaccgcc tggtgcgcat gtatcggtcc
     1021 atctttgcct tcggtggcgc cgaccagcgc atcttgaatg tcttgctgaa ctcggaatac
     1081 tacgaccggt taatcgtgca aggtagctcg tctccgatgt ggcgggaaga tcccaatggg
     1141 gcaaattatt tgcttaccgg catccaacaa atggctgagt acatcaatag caagggtatc
     1201 tcgaacagcc gccagaacct ggatggcatg cgctttgaac tccaacctcc cgcgggaggt
     1261 ggatcggcga gccaggtatt cgtccgctat tcgatcagcg cttacaatcg ttgggattat
     1321 gacccaacga ctgggtctta tctgcgttat gtggatacgc aggaggatac gggacaaggc
     1381 gagagttatg agcagctggt tgaccggttg acggagcagc caatatctgc ccagaatgtg
     1441 gtggtagttt tcgccgttca cgatgattac cttaggaatg ggcgatacaa tgtcgtggat
     1501 atcttgctcg atgtcagcgg tccagccttt atcttccggg atggacaggc atacgaggtt
     1561 cgctggtacc gccctcagct ggaatcggtt ctgtctctcc tagatctgaa tggcaaccca
     1621 tttccattta aacaaggcaa tacctggttc caggtgatcg gccaatcttc tcgctggggc
     1681 aacgaaagcg gagtatggcg cttcgaaagc cgcatcccat agaagtcatt ccagcctgcc
     1741 tcaagttagc ggtatgaaaa cgggtctttc tagcattgcc gtgctctggg cgttgatgat
     1801 ctcaggctgt gccgcggtcg cattagaccc gcaggtttca gtgtcttcta caccgccagc
     1861 tgaagtcagc gagattgcca ctgaagaacc aagcgctgct cttgaagtga tcgccacgat
     1921 ccctatcgcc actgagacgg ctacccccag cgtgacgctc gagccaccaa ccacggcaac
     1981 ctcatttgtg tatgcctacg ggccgagtga ttttcctgag ccgattaacc cactcaccgg
     2041 tctgccggtt gacccgccgc aactgttgga gcgacgcccg attgtcatca aggtgaccaa
     2101 ttttcctcgc agcgtacgac cacagtgggg gctatcacaa gcggatcatg tctacgaata
     2161 ttacattggc gatagcatgt cgcgttttgt gggggttttc tatggaaaag atgccgaacg
     2221 ggtagggcca atccgctcgg cgcgcctttt cgacgcgcat gtcatgcgca tgtatcatgg
     2281 catttttatc tttggatggg ctgatgacgc tgtgcttaaa ttcctgtttg cacctgactt
     2341 aaagttccac ctgattgtgg aacaggctga aaattgcccg cccctgtgtc gttttggcct
     2401 aaaagaagct tacaacactt tatatgccga cactgcacaa attgccgctt acttgacaaa
     2461 acgtaggaca aataatgaca ggcaaaatct gtccggcttg cgcttcgagt tggagacgcc
     2521 tgcgagcggt aatggaggtg agctgttctc gatccaatac tcattggtat cttatcaccg
     2581 ttgggagtac gaccccttgg caggtgtgta tttgcgctac caggagaccg gagctaaaaa
     2641 cctcagcgac agtgggtacg ccccgcttat ggatagcctg acggagagac agatttcggc
     2701 aaccaatgta attgtcatgc tggtgcccca ccaatatttt aagaaatcga attctaccga
     2761 gattatcgat caacccttca tggggcaggg ggctggttat gccttccgcg atgggcagat
     2821 atacccgctg atctggagac gccaggctgc cgaccagctg cccgagttgc tcttgccaaa
     2881 tgatcgcttg tacccgctca aacctggcaa cacctggttt gaaatcatcg gcgagacgac
     2941 acaatttgaa cagcttgaag acggcagctg ggaattcgta agctcgattc catagttagg
     3001 tttggctgca taacggcagc taattgctgc tgaagttcgg tatcagatcg cagcaatgat
     3061 ctttttcacg aggcttgggc cttcatagac caacccggtg tagacctgga ccagaacagc
     3121 gcccgcgtcg agcatctggc gtgcgtcact tgcgctcatc actccccctg caccaacgat
     3181 cgctaatttc cccccggtgt atttggcgat ctggcggatc atctgtgtgc taagctttcg
     3241 aagtggagct ccgctcaaac cgcccgtctc gactgcgagc ggggaacgca gcctatcccg
     3301 tgaaactgtt gtgttagcgg cgatgacccc atctagattg aaatgtagaa tggcctccag
     3361 ggcatcgtct agctcgccag aatttaaatc aggggcaagt ttgacgagca gaggtatata
     3421 gtttccggta gcgcgcgaga tctctttccg ccgcgtggtt agcgccctga gcagagtttc
     3481 caatgcctgg cgcgcttgca gtcggcgcag cccgagcgtg ttaggcgagc tgacgttgat
     3541 caccaggtaa tctgccaatg gagcaaatat atctatcagt accagataat catctgcagc
     3601 cctttcgagc tgcgtatcct tgttcacccc caggttaact ccgaggataa gtccaggcgg
     3661 acgctctcca gtgagctgct gtcgcacaaa gtcagcgcct ctgccaggga agcccatccg
     3721 attgatcagt gctcgatctt ctggcaagcg gaagaggcga gggcgcgggt tgccgtcctg
     3781 aggtcttggg gtcaccgtgc caacttcgat gtgcccaaag cccaggcagg ccagaccgcg
     3841 ccaggcaagc ccgtccttat cataaccagc agctaaaccg accgggttgc gaaatgtcaa
     3901 tccaaaagca tgcacaggct cttcagcagc ggcgaacaaa ttgcgcatgg cagctcgtag
     3961 aggggcaaac ctcccaacca tgcgcatggc agccaggctt agattgtggg cttgctcggg
     4021 ctcaagtgtg aacaaaagtg ggcgaaaaag attgtacatt caggggttgc ttcccagcga
     4081 acgggcaagt ttcacaccat attgctcgcc tcaaaaggca ggttagctgg catatcaatt
     4141 cccatctggc gtccctaaga attccctgac gatccgggct tgctcgccgg taatcgactt
     4201 tgacccctcc cacagattga tcaattcaag caaggtgtag atagcatgca tgcggtaacc
     4261 ggctcgattt agggcttctc cagcgccgga ctggcggtcg atgagtacga ccacatcttg
     4321 tacctgcagg ccagcatggg ttagcttctc gatggcctcg aatttgctgc cgccggtggt
     4381 cgccaggtcg tcgatgacga caacgcgctc gccttgtgaa tagacgccct cgatctcagc
     4441 ccgggtgcc
//
LOCUS       DBNHOH010000113         1015 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1287307, whole genome shotgun sequence.
ACCESSION   DBNHOH010000113 DBNHOH010000000
VERSION     DBNHOH010000113.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1287307"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="AC16V7_02065"
     CDS             complement(<1..632)
                     /locus_tag="AC16V7_02065"
                     /inference="COORDINATES: protein motif:HMM:NF036766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR6255930.1"
                     /translation="MTENKQGAIHTCQIAMMPSGRKGSVPQGINLLKAAQELGVELES
                     ICGGRQTCGKCQVVVEDGHFPKHALTSRADHLSAPTTREIEYSQEHRLQGRRLACAAE
                     VLGDVLISVPEESQARKQVIAKAASERSIEIDPAVRQVYVEMHPSDLSDSRGDWERLQ
                     EALEAQWGLKGLDIDSSALATLQIALREGDFSVTVNVWQEREVLRIQPGY"
     gene            complement(658..>1015)
                     /locus_tag="AC16V7_02070"
     CDS             complement(658..>1015)
                     /locus_tag="AC16V7_02070"
                     /inference="COORDINATES: protein motif:HMM:NF018012.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR6255931.1"
                     /translation="EIGPAGMFAGTEHTLERMRTTMLLAEVADRDPRAQWMERGGLDS
                     HARAMKRVKDILTRENPAAFSPELDEKVRAAFPGIVAGDSLPPEGWKRVTVVDEEGDA
                     GMRRRRRLHRAVATEA"
BASE COUNT          199 a          326 c          267 g          223 t
ORIGIN      
        1 tgatagccag gctggatgcg cagcacttcg cgctcctgcc atacgttcac tgtcactgaa
       61 aaatcgcctt cacgcaaggc gatttgaaga gtcgccaggg cgctactatc tatgtccagc
      121 cccttcaacc cccactgtgc ttccaatgct tcctgcaacc gctcccagtc accgcgcgaa
      181 tcgctcaggt ctgatgggtg catttctacg tacacttgac gcacagcagg atcaatctcg
      241 atgctgcgct cagaagcagc cttggcaatt acctgcttgc gcgcctggct ttcctcggga
      301 accgagatca gcacgtcacc cagcacttcg gcagcgcatg ccaggcgacg cccttgcagg
      361 cgatgctcct ggctatattc gatctcgcgg gtggtaggag cagaaaggtg atcggcgcgc
      421 gaggtgagag catgctttgg aaaatgccca tcttcgacga ccacctggca cttgccgcag
      481 gtttgccgcc caccacaaat tgattccagc tccacgccga gctcctgggc tgccttgagc
      541 aaattgatgc cctgaggcac ggaacccttg cgcccagaag gcatcattgc aatctggcag
      601 gtatggattg cgccctgttt attttccgtc acgagttttg cctgctatgt tgactcatta
      661 tgcctctgtc gccactgccc gatgaaggcg ccggcggcgg cgcataccgg catcgccttc
      721 ttcatcaaca actgtgacgc gcttccaacc ctcgggcggc agcgaatcgc ccgctacaat
      781 accagggaaa gctgccctta ccttttcatc cagctcggga gagaaagctg ctggattttc
      841 acgcgtcagg atatctttga cgcgcttcat ggcgcgcgca tgcgaatcca acccacctcg
      901 ttccatccac tgggcgcgcg gatcacggtc agcaacctct gccaggagca tggtggtgcg
      961 catgcgttcc agagtatgct cggttccggc gaacatgcct gcaggcccga tctct
//
LOCUS       DBNHOH010000114         1970 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_582219, whole genome shotgun sequence.
ACCESSION   DBNHOH010000114 DBNHOH010000000
VERSION     DBNHOH010000114.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_582219"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..852
                     /locus_tag="AC16V7_02075"
     CDS             31..852
                     /locus_tag="AC16V7_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6255932.1"
                     /translation="MASWLGWQIESNWADPFLFAVYSFAKPLAGAAILVVMYSVITQG
                     DFDSPIFPYMYLGNAFYIYVGQLMTGISWAVIDDREHYKTLKYIYAAPVNFPVYLLGR
                     GVARLLIASISVVVTILFGVFFLRLNIDFSAVNWPLFLVTLLIGVFMLSLMGLILAGA
                     MLLLVHHMWELGGAVAGALYLFSGAIFPLSVLPAFLRPIGYLMPITYWLELLRRSLVG
                     SVAQAFPTLAGFNNSQLMGILLALTIAFAFISVGSFRWCENKARERGVIDVVTNY"
     gene            859..1371
                     /locus_tag="AC16V7_02080"
     CDS             859..1371
                     /locus_tag="AC16V7_02080"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR6255933.1"
                     /translation="MMKNIYIGEVAPSPLGPIWVGLSERGLLAVEIQASRDAFCAHLL
                     RKFPGARVIIDQDRTAQAVQQIGEYLSGERRSFDLSIDWSGMTAFQEQALRVTLGIPY
                     GQVTTYGEIARQIGRPRAARAVGRAEATNPIPLVVPCHRVLGKDGGLHGFGAPGGVKT
                     KAWLLQLEGK"
     gene            1384..>1970
                     /locus_tag="AC16V7_02085"
     CDS             1384..>1970
                     /locus_tag="AC16V7_02085"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR6255934.1"
                     /translation="MLRNLFIRLGLLNSILGITVLTILISILLTSGFSALIGDGPDLT
                     GLWTAIFVPAIITPIFSSIFLRMLFQFVSTESQLRELNRRDSLTGVYNRAYFIELAER
                     ELVRTLRYGETFSIAIIDIDDFKRVNDTYGHLAGDKALQVISEVSLHNLRQTDTIARF
                     GGDEFVVLFPYAGSQHVHECVERIRRILADTPIPFA"
BASE COUNT          390 a          448 c          552 g          580 t
ORIGIN      
        1 cggtaaatga tgatgtggcg ctcgtttcgc atggcttcct ggttgggctg gcagatcgaa
       61 tcgaactggg cagatccgtt cctgttcgca gtatattcgt tcgccaaacc cttggcaggg
      121 gcggctattc tggtggtgat gtatagtgtc attacccagg gggattttga ctcgccgatt
      181 tttccgtata tgtacttagg aaatgctttt tatatctatg tcgggcagct gatgacgggc
      241 atttcctggg ctgtgattga tgatcgcgag cattataaga ccttgaagta tatctatgct
      301 gctccagtca atttccctgt ctacttgctg gggcggggtg tggcacgctt gctgatcgcc
      361 tccatctcag tagtagtgac catcctgttc ggtgtattct ttctgcgcct gaatattgat
      421 ttctcagctg tcaactggcc gcttttcctg gtaactctgc tcattggtgt gttcatgtta
      481 tcgctgatgg gcttaatcct ggcaggagcg atgttgctgc tggtgcacca tatgtgggaa
      541 cttggcggcg cagtggcagg tgccctgtat ctgttcagcg gggcgatttt tcccctgagt
      601 gttttgccag ctttccttcg tcctattggc tatttgatgc cgattactta ttggctggag
      661 ttactgcgac gatctctggt gggctctgtg gcacaggctt ttcccacgct tgccgggttc
      721 aacaattctc aattaatggg tatcttgttg gcgctgacta tagcatttgc atttatttct
      781 gttggctcat ttcgctggtg tgagaataag gcgcgtgagc gcggggtaat agatgtagtg
      841 acgaattatt gagtaacaat gatgaaaaat atttacattg gtgaagtagc accctccccc
      901 ttgggtccca tttgggtagg gctatcggag cgtgggctgt tggctgtcga gatacaagct
      961 agccgggatg cgttttgtgc gcatttgctc cgcaaatttc cgggtgcccg ggttataatc
     1021 gatcaagatc gtactgcgca ggctgttcag cagattggag aatacttgtc gggggaacgc
     1081 cggagttttg acctttctat tgactggtct gggatgaccg catttcaaga gcaggcactg
     1141 cgggttacgc tgggcattcc ctacggtcag gtgacaactt acggcgaaat tgcccgtcaa
     1201 attggcaggc cgcgcgctgc tcgtgctgta ggccgcgctg aggctaccaa ccctattccc
     1261 ttggttgtac catgtcatcg tgtgctgggt aaggacggtg gcttgcatgg attcggcgca
     1321 ccaggtgggg tgaagactaa agcctggcta cttcaacttg aaggaaagta aacaaaagat
     1381 cgcatgttgc ggaatctgtt catccgtttg ggtctgctaa actcgatatt ggggatcacc
     1441 gttctaacga tcctgatttc gattctgttg acaagtggat ttagtgcatt aatcggagat
     1501 ggtcctgatc tgaccggact ctggacagcg attttcgtcc ctgcgatcat tacaccgatc
     1561 ttcagttcga tattcctgcg tatgttgttt caatttgtca gcaccgaaag tcaattgcga
     1621 gaactaaaca ggagagatag cctgaccggt gtgtacaatc gagcgtattt cattgaatta
     1681 gccgagcggg agttggtgcg taccttgcgc tatggagaaa ctttttcgat cgctatcatt
     1741 gatatcgatg atttcaagcg cgtcaacgat acctatggcc atctggctgg cgataaagcc
     1801 ttacaggtga tcagcgaggt cagcttgcat aatctgcgcc agacggatac catcgcccgt
     1861 ttcgggggcg atgagtttgt ggtcctgttt ccatatgcgg gtagtcagca cgttcacgag
     1921 tgcgttgagc gtatccgcag gatcctggcc gacacgccca tccccttcgc
//
LOCUS       DBNHOH010000115         1996 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_774716, whole genome shotgun sequence.
ACCESSION   DBNHOH010000115 DBNHOH010000000
VERSION     DBNHOH010000115.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_774716"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="AC16V7_02090"
     CDS             complement(<1..879)
                     /locus_tag="AC16V7_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003481239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6255935.1"
                     /translation="MTNYGLFQLSPEHEMIRQAARDFAQKEIAPIAAHFDETGEFPYT
                     TIKKMGEMGFMGVEVPEEYGGPGMDTLAYVLALEEICKADASHGTIMSVNNSLFCHGL
                     NKFGTEEIKQKYLRAVASGEAVGAYSLTEPMSGSDAGNMRSRAVREGDHYVLNGRKSW
                     VTSAPVAEYIVVFTMTEPEKKHRGITAFLIESNKPGFQQGKKEPKLGIRASATSEIVF
                     EDYKVPVENRLGEEGEGFKIAMNVLDAGRIGIAAQGLGIAEAAYEASLSYSTEREAFG
                     QKIGEFQGISFKLADMK"
     gene            1022..1339
                     /locus_tag="AC16V7_02095"
     CDS             1022..1339
                     /locus_tag="AC16V7_02095"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF014716.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIR6255936.1"
                     /translation="MTEPILPDLFNHLVQLAALELSPEEAEYLRRELNNQLKAIHELE
                     AIPLGADIPAASHGVSYTEDTSPAIRQDEWHPHPNPDEILEQAPETDEGYIIVPDIPH
                     TEL"
     gene            1437..>1996
                     /locus_tag="AC16V7_02100"
     CDS             1437..>1996
                     /locus_tag="AC16V7_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR6255937.1"
                     /translation="MELCDLPAHILAENIRLRQVKAIDALEAALARIAAVDGDPPTLE
                     PADLDSDDHVHAFITVTAERARLQAREIDRRLDAGEDVGPLAGVPFTVKDIFCVKGTP
                     STAGSRILANFTAPYTATPVERMEAAGGVMLGKVNLDEFTYGSSNESSAFHPTPRNPW
                     NVQRVPGGSSGGSAAAVAAGEGALSLG"
BASE COUNT          409 a          582 c          511 g          494 t
ORIGIN      
        1 cttcatatct gccaatttaa aggaaatgcc ttggaattcg ccaatctttt gcccaaaggc
       61 ttcgcgctct gttgaataag ataggctggc ttcataggca gcctcggcaa tgcctaaccc
      121 ctgggccgcg atgccgatgc gtccggcatc cagcacgttc atggcgattt tgaagccctc
      181 gccttcttcg cccagccggt tttctacagg gaccttgtaa tcttcaaaga cgatctcgct
      241 ggtggctgag gcgcggattc ccaatttggg ttccttctta ccctgttgaa aacctggctt
      301 gttgctctcg atgaggaatg ctgtgatgcc gcggtgtttc ttctctggct cggtcatggt
      361 aaagaccacg atgtactctg ccacgggcgc gctggtgacc cacgatttgc gcccattgag
      421 cacataatgg tcaccctcgc gtacagcccg gctgcgcata tttccagcgt ctgagccgga
      481 catcggctcg gttagcgagt aagcgcccac ggcttctccg gatgctaccg cgcgcaagta
      541 cttttgcttg atctcttccg tgccgaattt atttaagccg tggcaaaaca gagaattgtt
      601 caccgacatg attgtgccgt gtgaggcatc cgctttgcag atctcctcta gtgccagcac
      661 gtaagccagg gtgtccattc caggtccacc gtattcctct ggcacttcaa cccccataaa
      721 gcccatttcc cccatctttt tgatggttgt ataaggaaac tcgcccgtct cgtcgaaatg
      781 cgcggcgatg ggggcgatct ctttttgggc gaagtcacgc gcagcttgcc ggatcatttc
      841 atgctcgggg ctaagctgga ataagccgta gttggtcata ttctcctcct tagatcaaag
      901 ctgctgccag attcattata gcatgcctct agatgatata cgtttctttt cgcttgcgtt
      961 ggcactcaaa gtcggatata ctgccttgcg gagtgagcta acccaaattg gagcttgatg
     1021 aatgactgag ccgatactcc ccgacctttt taaccacctc gtccagcttg ctgcccttga
     1081 gctttctcct gaagaggctg aatacctgcg gcgtgagcta aacaaccagc taaaagccat
     1141 ccacgaactg gaagccatcc cactgggagc tgatatccct gccgcttcac acggtgtttc
     1201 ctatacggaa gacacctccc ctgccatccg ccaggatgaa tggcatcccc atcctaatcc
     1261 ggacgagatc ctcgaacaag cccctgagac cgatgaaggt tatatcatcg tcccagacat
     1321 tccccatacc gaactctgat ttttagtgtt ccaggtgtca agtgccacgt tcacctgctt
     1381 attggaactt ggaacttggt acttagaacc tagatcttgt tacctgaaac atacccatgg
     1441 aactttgcga cttacccgcc catatccttg ctgaaaacat ccgcctgcgc caggtgaaag
     1501 caatcgacgc gctggaagcg gcgctggccc gcatcgctgc agtggatggc gatcccccga
     1561 ctttggagcc tgcggacctt gattctgacg atcatgtcca tgctttcatc accgtaacgg
     1621 cggagcgtgc ccgcctccaa gctcgcgaaa tcgaccgccg gctggatgct ggtgaagatg
     1681 tcggtccgct ggcaggcgtg ccattcacgg tgaaagacat cttttgtgtg aaggggacac
     1741 cttcaacggc tggctcgcgc attctggcaa actttaccgc gccatatacc gccactccgg
     1801 tggaacgcat ggaagctgct ggtggggtga tgctgggcaa ggtaaatttg gacgaattca
     1861 cctatggctc atccaacgag tcgtcggctt tccatcccac cccgcgcaac ccctggaatg
     1921 tacagcgcgt gccgggcggc tcatcgggtg gtagcgccgc ggcggtagca gccggggagg
     1981 gtgctctttc tctggg
//
LOCUS       DBNHOH010000116         5203 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_582265, whole genome shotgun sequence.
ACCESSION   DBNHOH010000116 DBNHOH010000000
VERSION     DBNHOH010000116.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5203)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_582265"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1615
                     /locus_tag="AC16V7_02105"
     CDS             <1..1615
                     /locus_tag="AC16V7_02105"
                     /inference="COORDINATES: protein motif:HMM:NF013727.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIR6255938.1"
                     /translation="PIQGQRSGGATEAKKKKRMHLPVEFRQKFQVPADEASSDDAISP
                     PDRDERLPPFDLLVHEQTARPDERTINETAYLIEKTLTEFGIPAKVVGFRIGPTVTQF
                     AVEPGFVEKGSSNEGEANRQKVRVSQISALSRDLALALSAERLRIEAPVPGRPYVGIE
                     VPNARSTVVRLRPVLESESFIKISSPLAIALGRDVSGQPVVTDLSRMPHLLIAGTTGS
                     GKSVCIAALTTCLAMNNTPEDLRLVMIDPKMVELVRFNGLPHLYGRVETDLERILGVM
                     RWTVAEMDRRYRLLEATRSRNLDTYNRKMRRRKGEAPLPRIVVLIDELADLMMSTPDQ
                     IEPTLVRLAQMARATGIHLVVATQRPSTDVVTGLIKANFPARLSFAVASSVDSRVILD
                     TVGAETLLGHGDMLFLPPESPGPMRAQGVMVSDQEVERVITFWQKSFPQAEVEPPPWE
                     EMLKQESMLADRDDLIEQAIKIVRENQRVSASFLQRRLRIGYPRAARLIDELEELGIV
                     GPSQGGGREREVFIDPDEGEEFQDYHVED"
     gene            1737..2354
                     /locus_tag="AC16V7_02110"
     CDS             1737..2354
                     /locus_tag="AC16V7_02110"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR6255939.1"
                     /translation="MSENVVNQKKPLTLTDYLRVQFKWLLDSIAAFLNRLGLMPNTMT
                     ILGLIGNTLGAFFLARGQMTLGGILILLMGPVDALDGAMARLRGEPTEFGAFVDSVTD
                     RYSELVIFGGLIYFYMQRGDWLMVALAYAAAAGSVLVSYTRARAQSLGLETKVGLLTR
                     LERYLVLSPALVFNIPHIALWILAFLTNLTALQRIFDVRSKARTK"
     gene            2406..3203
                     /gene="lgt"
                     /locus_tag="AC16V7_02115"
     CDS             2406..3203
                     /gene="lgt"
                     /locus_tag="AC16V7_02115"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR6255940.1"
                     /translation="MPSQGFYVGPVFIYYYGIILMLGAVAASYLAEREARRRGQNGEL
                     VWDALIWVLIAGIVGARLWHIFTPPPSMVEQGITTKFYLTHPLDALWIRRGGLGIPGA
                     VIGGVVALYWFCRRRKLNFAEWADIAAPALALGQAIGRWGNFTNQELYGAPTSLPWGI
                     TIQYAPGYPPETRFHPLFLYESLWNLANMFLLLWLARRFSDRLKVGDLFLVYLIIYPL
                     GRFLLEFMRLDSALVGGININQTIMVVVALLAAGVLYWRHRKAVSAV"
     gene            3337..4275
                     /locus_tag="AC16V7_02120"
     CDS             3337..4275
                     /locus_tag="AC16V7_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6255941.1"
                     /translation="MIVTHQLGKQFDDFWAVDEVSLHVKAGEVLALLGPNGAGKTTTV
                     RMLTSILRPTRGEARVAGYDVVTQAARVRASVGVLTEQHGLYGRMPAGDYLDFFGEVY
                     GLDRSVRQARILDLLENFGLLADRDRRIGEFSKGMRQKLALARALLHDPPVLLLDEPT
                     SAMDPESARLVRDSIRELRSASRAVIICTHNLSEAEELADHIAIIRRGRIIAQGSPQT
                     LKANFLGPAEYEVRLGVNLNGQVLSLPHGAKLTSQGEDWLRFRTLEPALANPAILRYL
                     LDLGLPVLGLQEVARSLEEVYLKAVSVAEGEEGTDV"
     gene            4268..>5203
                     /locus_tag="AC16V7_02125"
     CDS             4268..>5203
                     /locus_tag="AC16V7_02125"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6255942.1"
                     /translation="MFEAARQQLMPALIITRREVRDQFRDWRIIFPIVILTIFFPVLM
                     NFTAKQAISFVDRYGAPIVVDRLIPFLLMVVGFFPISVSLVIALESFVGEKERYSIEP
                     LLCTPLSDWQLYLGKLLAATIPPLLASYLGIGVYVVGIFTQVGWQPDFLLLTQIVLLT
                     TVQALVMVCGAVVISSQTTSVRAANLLASFIVIPMALLIQGESIIMFWARYNVLWWVI
                     FGQVLIAGLLLRVGITKFNREELLGRELDTLNLRWSWRIFKSKFIGQARSPLTWYRLE
                     IAGTVRAMTVPIGIMSLALIAAIMIGVEQARVFVIP"
BASE COUNT         1082 a         1266 c         1496 g         1359 t
ORIGIN      
        1 ccctattcaa ggtcagcgat caggtggagc aacggaagca aaaaagaaga aacgaatgca
       61 cctgcctgtt gaattccgcc agaaatttca ggtccccgcg gatgaagcaa gcagcgacga
      121 tgcaatctct ccccctgatc gagatgagcg tttgccacca tttgacctgc ttgtgcatga
      181 gcaaaccgcg cgccctgatg aacgtacgat caatgagacg gcgtatctca ttgaaaagac
      241 gctcacagaa tttggcatcc cggcaaaggt ggtgggattc cgtatcgggc cgactgtgac
      301 gcagtttgct gttgagcctg ggtttgtgga gaaagggtcc tcaaatgaag gcgaggctaa
      361 ccggcagaag gtgcgtgttt cgcagatatc tgccttgagc cgcgatttgg ctctggccct
      421 gtctgccgag cgcttgcgta tcgaggcccc tgtgccgggc agaccatatg tgggtattga
      481 agttcccaat gctcgctcga cagtggttcg attgcgccct gttttggaaa gcgagtcatt
      541 cattaagatt tcttcgccac tggctattgc cctggggagg gatgtctccg ggcagccggt
      601 tgtgacagat ctatctcgta tgcctcacct gctgatcgct ggaaccacgg gttcaggtaa
      661 atcggtttgc atcgctgctt tgacgacatg cttggcaatg aacaacaccc cggaagacct
      721 gcgcctggta atgatcgatc ccaagatggt cgagctggtg cgctttaatg gcttgccaca
      781 tctatatggg cgggtagaga cggatcttga gcgcattctg ggcgtaatgc gctggacggt
      841 ggcggagatg gatcgccgct accgattgtt ggaagccaca cgttcgcgca atctggatac
      901 ctataaccgc aaaatgcgcc ggcgaaaggg tgaagcgccg ttgccgcgca tcgttgtgct
      961 gatcgatgag ctggctgatc tcatgatgtc cactccagac caaattgaac cgacccttgt
     1021 gcgcctggct cagatggcac gcgccacagg cattcacctg gtggtcgcca cccagcgtcc
     1081 gagcaccgac gtggttactg ggttgatcaa ggccaacttc ccagcccgtc tttcctttgc
     1141 agtggcatcc tcagtcgatt cgcgggtaat cctggatacg gtcggggccg agactttgct
     1201 ggggcatggt gatatgctct tcttgccgcc tgaatcccct ggtccaatgc gggcgcaagg
     1261 tgtaatggtt agcgaccagg aagtggaacg ggtgatcacc ttttggcaga aaagctttcc
     1321 ccaggctgaa gtcgagccgc ctccttggga ggagatgctc aagcaggaat ccatgctggc
     1381 agaccgtgac gacctgattg agcaggcgat caaaatagtg cgcgaaaatc agcgggtaag
     1441 cgcctcattt ttgcagcgcc gcttgcggat tggttatccc cgtgctgccc gattgatcga
     1501 cgaactggaa gagttgggca tagttggccc atcccaggga ggtgggaggg agcgcgaggt
     1561 gttcatcgat ccagatgaag gcgaggagtt tcaagattat catgtggaag attaaaggct
     1621 gggtggtcat ttctatgctt ttatctgatg ctcatatttg attacttgac atccacatga
     1681 cctgctgata gcatactccg aatgccatta tgcctcattg aaaggtgatc tcgaaattgt
     1741 ctgaaaacgt agtgaatcaa aaaaaaccat taacccttac tgactatcta cgggtgcaat
     1801 ttaagtggct gttagatagt atcgccgcgt tcttgaatcg actgggtttg atgcccaata
     1861 cgatgaccat tttgggtctg attggcaata cgcttggagc atttttcctg gcgcgtggtc
     1921 aaatgaccct cggcggaata ctgatcttgt tgatggggcc cgtcgatgcg ctggatggcg
     1981 ccatggccag gttgcgcgga gagccgacag aatttggcgc ttttgtcgat tcggttactg
     2041 atcgctattc agagttggta atcttcggcg gtctgatcta cttttatatg caaagagggg
     2101 attggctgat ggtggcactt gcctacgccg ccgccgccgg gtctgtgcta gtatcgtaca
     2161 cacgcgcccg ggcgcaatcc ttgggtttgg agaccaaggt ggggttgctt acccgtttgg
     2221 agcgctatct agtgctctcg ccggcattgg tcttcaatat tccacatatc gctctttgga
     2281 tcctggcttt ccttacaaat ctaaccgcct tacagcggat atttgatgtg cgtagcaaag
     2341 ctcgaacgaa ataacccggg gtagaagaca cgataacaat tagatcctaa ttctgaggga
     2401 gaaccatgcc atctcaaggt ttttacgttg gaccggtatt tatctactac tatggaatca
     2461 tattaatgct cggcgcagta gcagcctcat acctcgcaga acgtgaagcc cgccggcgcg
     2521 ggcagaacgg tgagctggta tgggatgcat taatctgggt gttgatcgcc gggattgttg
     2581 gggcgcgctt gtggcacatc tttacaccac ctccctcgat ggtggagcaa gggattacta
     2641 caaagttcta cttaacccac cccctggatg cgctgtggat acggcgcggt ggtttgggca
     2701 tccctggtgc ggtgatcggc ggcgtggtgg cgctatactg gttttgccga aggcgcaagc
     2761 tcaattttgc cgagtgggcg gacatcgctg cgccagcact tgcattgggg caggccatcg
     2821 ggcgctgggg taatttcacc aaccaggaat tatatggcgc gcccaccagc cttccctggg
     2881 gcataaccat ccagtatgcg ccgggatatc cgccggagac gcgttttcat cccctttttt
     2941 tgtatgagtc tttgtggaac ctggccaata tgttcctgtt gctctggttg gcgcggcgct
     3001 tctccgatcg cctgaaggta ggtgatttgt ttctggtgta cttgataatc tacccgctag
     3061 gtcgcttcct gctagaattt atgcgattgg attccgccct ggtgggtggt atcaacatca
     3121 accagaccat catggtggtg gtcgccttgc tggctgctgg cgttttatat tggcgccatc
     3181 gcaaggcagt gtctgctgtt taatgatgat gtacttgtat ttcaactgta tatttcccca
     3241 ctcatctaat tacaggactg ttgtcttatt gaaaaattcg gttgaaatga atatagtagg
     3301 ttatgtgggt gttatcatgc ggggttcata cgaaagatga tcgtaactca ccaattaggt
     3361 aagcaatttg atgatttttg ggcagttgat gaggtaagcc tgcacgtgaa ggctggcgaa
     3421 gtgctggctt tgttaggtcc aaatggggca ggtaagacca ccacagtgcg catgttgacc
     3481 tccatcttgc gacccacgcg cggcgaagcg cgcgttgcag gttacgatgt agtaacccaa
     3541 gcggcacgtg tgcgggcttc ggtgggcgtg ctgaccgagc agcacggctt gtatggtcgc
     3601 atgccggctg gcgattacct tgacttcttt ggggaggtat atggcctgga tcggtcagtc
     3661 cggcaggcgc gcatcctcga tttactggaa aattttgggt tgctagcaga ccgcgatcgg
     3721 cgcatcggtg aattttcgaa ggggatgagg cagaagttgg ccttagcgcg tgccttgttg
     3781 catgaccctc cggtgttgtt gctggacgaa cccacctcag ccatggaccc cgaaagcgcc
     3841 cgcctggtgc gcgattctat tcgggaattg cgttcagcca gccgggcagt tatcatctgc
     3901 actcacaacc tgtctgaagc agaggaactg gcagatcata ttgctatcat ccgcagaggc
     3961 cgtattatcg cccagggatc gccgcaaact ttgaaagcaa attttttagg accggctgag
     4021 tacgaagtcc gtttaggagt caacttgaac gggcaggtgc tttctctccc acatggagcg
     4081 aagctgacat ctcagggaga ggactggctg cgctttcgta ctttggaacc tgcgctagct
     4141 aatccggcta tcctgcgtta cctgcttgac ctgggcttgc ctgtgctggg gttgcaggag
     4201 gtggcgcgca gcctggagga ggtttacttg aaagctgtca gtgttgctga gggtgaggag
     4261 gggaccgatg tttgaggctg cccgccaaca gctcatgccg gcgttgatta ttacccgccg
     4321 tgaggtgcgt gatcagttcc gtgactggcg catcatcttc cctatcgtca tcctgaccat
     4381 cttcttcccc gtactgatga atttcactgc caaacaagcg atctcatttg tggatcgtta
     4441 tggtgcgcct attgtagtcg atcgcttgat ccctttcctg ctgatggttg ttggtttttt
     4501 ccccatctct gtatcgctgg ttattgccct ggagagtttt gtcggagaaa aagaacgcta
     4561 tagtattgag ccgctgctgt gcaccccatt gagtgactgg cagttgtatc tgggcaagtt
     4621 attagctgca acaattccgc ccttattggc cagctacctg ggcattggcg tttacgtggt
     4681 tggcatattc acgcaggtgg gatggcagcc cgattttcta ttgctgacac agatcgtgtt
     4741 actcactaca gtccaggcac tggtgatggt gtgcggagca gtggttatct catcccagac
     4801 tacctcagtg cgcgcagcga atctgttggc ctcttttatc gtcataccaa tggcattgtt
     4861 gatccagggg gagagcatta ttatgttctg ggcgcgctac aacgtgctgt ggtgggttat
     4921 ctttggacaa gtgctcattg ctggtctgtt gttacgggta ggaataacta aattcaaccg
     4981 cgaagagctt ttaggcaggg aattggatac cctgaatctc cgctggagct ggcggatatt
     5041 caagagtaaa tttattggcc aggccaggtc acccctgacc tggtaccgac tggaaattgc
     5101 cggcactgtg cgtgcaatga ctgttccaat cggcataatg tcattggcat taattgctgc
     5161 tattatgatc ggtgttgaac aagcgcgggt gttcgtcatt ccc
//
LOCUS       DBNHOH010000117         2613 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_261471, whole genome shotgun sequence.
ACCESSION   DBNHOH010000117 DBNHOH010000000
VERSION     DBNHOH010000117.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_261471"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /gene="lepB"
                     /locus_tag="AC16V7_02130"
     CDS             complement(<1..575)
                     /gene="lepB"
                     /locus_tag="AC16V7_02130"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR6255943.1"
                     /translation="MDNNIDSISIETADDQAAIPQTKTKIVRFLVDIVETLVFSLLLF
                     AGINALTARIRVDGMSMEPTLHSGEFVIVNRIAYRFGQPAIGDVIVFLFPRDPTQEYI
                     KRVIGLPGDEVVISNSQVQVNGQALTEPYIAAPPKYESQWTVPEGSLFVLGDNRNNSS
                     DSHTWGPVPLENVIGKAVFVYWPPVEWGVVE"
     gene            complement(629..2002)
                     /gene="der"
                     /locus_tag="AC16V7_02135"
     CDS             complement(629..2002)
                     /gene="der"
                     /locus_tag="AC16V7_02135"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR6255944.1"
                     /translation="MPKPVVVLVGRPNVGKSTLFNRLAEERLAVVDETPGTTRDRLMA
                     EAEWAGVAFDIVDTGGIDPSLMASGRKKEPLSIGSADYIGQIRAQAEMAIQEADAVLF
                     VTDAEDGVTPADREVAQILRRHQREVDGESWPPVFLVVNKADSEARRAQALQFYELGM
                     GDPHPISALHGTGTGDLLDVLVASFREPGEEAEDDTVKIAIVGKPNVGKSSLLNRLVG
                     QERAIVSPIPGTTRDAVDEQIMVGEIPVTLIDTAGIRRRGRIEPGVEKYSVLRSLRAI
                     ERADVSLLVVDATSGMTAQDTHIAGFILDAWKSAVVLVNKWDAIPKDSYTMDHFTRQV
                     RQELNFMDYVPVLFISAKTGQRVEQVLPLALRVQEERLVHISTSDINRIIQDAQRRHA
                     APSHAGRQLKMYYGTQVRSDPPTFMIYVNDPKLAHFTYRRFLENRVREAYGFLGTPIR
                     IVLKPRR"
     gene            complement(2013..>2613)
                     /locus_tag="AC16V7_02140"
     CDS             complement(2013..>2613)
                     /locus_tag="AC16V7_02140"
                     /inference="COORDINATES: protein motif:HMM:NF019561.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HIR6255945.1"
                     /translation="VVKVTTAGQVAQGYWLTNVSVTPPNVTVFSTNPRLVNELPGFVE
                     TNPLDLTDLSDDIDMRTTLNLPEGVTLAGEESVLVRLSIAALEGSLPITLPLEVVGLS
                     PEYQAKLSPDTVDLLLTGPLPLLNNLTPGGIRVSVNLSGLEPDVHQVPPKVDLLPPQV
                     SVASIIPENVEVTIEIAPPETITPTGQGSPIPATVTPTP"
BASE COUNT          622 a          739 c          635 g          617 t
ORIGIN      
        1 tgttccacca caccccactc cactgggggc caatagacaa aaactgcttt tccaatgaca
       61 ttttctaacg gcaccggtcc ccaagtatgc gaatcagacg aattattacg attgtcacca
      121 agcacaaaaa gcgagccttc cggcactgtc cattgacttt catacttcgg aggtgcagcg
      181 atatatggct cggtcaaagc ttgaccgttt acttgaacct gactattcga gatcaccacc
      241 tcatctcccg gcagaccgat gacacgcttg atatattcct gcgttggatc tctagggaat
      301 agaaatacaa taacatcgcc aatcgctggc tgcccaaatc gatatgcaat tcggttcaca
      361 atgacaaatt ccccggagtg aagcgtaggc tccatactca tcccatccac gcgaatccgg
      421 gctgtaagtg cgttaatccc cgcgaagagc agcagagaga ataccagggt ttctacaata
      481 tcgaccaaaa atcgaactat cttcgtcttg gtttgtggaa tagcagcctg gtcgtcggcg
      541 gtttcaatac ttatgctgtc tatattatta tccatagtta atcaggcgga ttataaggcg
      601 ggtaagcagg tggtgcaagg gctgcaggtt acctgcgggg tttcagcaca atccttatcg
      661 gtgtacctaa aaacccatac gcttcacgga cgcggttctc taaaaatcgc cggtaggtga
      721 agtgcgccag cttgggatca tttacataga tcatgaatgt aggcgggtcg ctacgcacct
      781 gagtcccata gtacatcttg agttgtcgcc ctgcgtgcga aggcgcagca tgccgccttt
      841 gtgcatcttg aataatacga ttaatatccg atgtcgagat gtgcactaag cgctcctctt
      901 gcacgcgcaa tgccagtggc aacacctgtt cgacacgttg gcctgtttta gctgagatga
      961 acaacaccgg gacgtaatcc ataaagttta actcctggcg tacctggcga gtgaagtggt
     1021 ccatggtgta gctatctttg gggatggcat cccacttgtt caccagcacc acagcgctct
     1081 tccatgcatc caggatgaaa ccagcaatat gcgtgtcctg agctgtcata cctgatgttg
     1141 catccactac cagcaggctg acatcggcgc gctcaatggc gcgcagagag cgcaacacgc
     1201 tatatttttc cacgcccggc tcgatgcgtc cccgtctgcg gatgccagcc gtatcaatca
     1261 gggtaacggg tatctcaccc accatgatct gctcgtctac ggcatcccgc gtggtacctg
     1321 ggatcgggct gacaatggca cgctcttgtc ccaccaaccg gttcaataaa ctagatttgc
     1381 ccacgtttgg tttgccaaca atggcgattt tcaccgtgtc atcttcagcc tcttcgcccg
     1441 gctcccgaaa cgaggcgacc aggacatcca gcaaatctcc tgtaccggtg ccatgcaacg
     1501 ccgagatcgg gtggggatca cccatgccaa gttcgtagaa ctggagcgct tgcgctcggc
     1561 gggcttcact atccgcttta ttaactacca aaaataccgg gggccaagat tctccatcaa
     1621 cctctcgctg atggcggcgc aagatttggg ctacctcacg atctgcaggc gtcaccccat
     1681 cttctgcatc cgtaacgaaa agcacagcgt cagcctcctg gatagccatc tcagcctggg
     1741 cacggatttg cccaatgtaa tccgccgatc caatcgataa aggctctttc ttcctcccgg
     1801 aagccatgag ggaggggtca atgcctcccg tgtctacgat gtcgaaagcc acccctgccc
     1861 actcggcctc tgccataagc cgatctcgag tggtgccagg agtctcgtcg accaccgcca
     1921 ggcgttcttc tgccagacga ttaaacaagg tgctcttccc tacattcggg cgtccgacca
     1981 ataccacaac gggtttgggc atcacatatt ccttatggtg tcggagtgac tgtagctggt
     2041 atgggtgaac cctgacccgt aggcgttatg gtctcaggag gggcaatctc aattgtcact
     2101 tcaacatttt caggtattat ggaagcgaca ctgacttgag ggggtaacaa atccaccttc
     2161 ggtggaactt ggtgcacgtc aggctccaaa ccacttaagt taaccgagac gcggatcccc
     2221 ccaggtgtca agttgttcaa cagaggtaat ggacccgtca agagtaaatc cacggtatct
     2281 ggtgacaatt tcgcctggta ttcaggtgac agaccgacta cctctagggg caatgtaatg
     2341 ggtaggcttc cctccagcgc agcgatgctc aggcgtacca gcacactctc ctcgcccgct
     2401 aaagtaaccc cctcgggcag gtttagcgtc gtacgcatat cgatatcatc actcaaatcg
     2461 gtcaaatcca atgggttcgt ctccacaaaa ccgggtaact catttaccag cctaggattg
     2521 gtggagaaca cagtgacatt gggtggtgtg actgatacat tagtcagcca gtatccctga
     2581 gccacttggc ccgccgtggt cactttcacg acc
//
LOCUS       DBNHOH010000118         1831 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1159726, whole genome shotgun sequence.
ACCESSION   DBNHOH010000118 DBNHOH010000000
VERSION     DBNHOH010000118.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1159726"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="AC16V7_02145"
     CDS             complement(<1..469)
                     /locus_tag="AC16V7_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255946.1"
                     /translation="MRKQGCLISLIALPTTCLLCVGVYFLPPVQARLGWRVDNWLADL
                     RRSLAPEGQVVFVPKEQNEQVEAIVQATLQALLQSSPTTGADMPAQNPIANLTPTALP
                     GEPTATLEPPPTSTVTPTPIPEKMILEGIRYQRQTFNNCGPANLAMALSYWGWQ"
     gene            complement(534..1106)
                     /locus_tag="AC16V7_02150"
     CDS             complement(534..1106)
                     /locus_tag="AC16V7_02150"
                     /inference="COORDINATES: protein motif:HMM:NF046921.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkoP family protein"
                     /protein_id="HIR6255947.1"
                     /translation="MVRNFDAWVRQGRGVFEFSQDPGCLLRIQLATARIELDLPDRVV
                     SPGDAVLLLHLWNERLLQFPPQGPDLAWAKPMQRQFVHSLRMVATCLKESSELAHVSA
                     VGGVTVLAGLGTENGGSRLLKRLGFKLIPYTHPLGTFGEFWENFYSWVLIWTYNPSAL
                     RYRSLWGLKRIEFWMSVRAFLERFGYIAYP"
     gene            complement(1118..1501)
                     /locus_tag="AC16V7_02155"
     CDS             complement(1118..1501)
                     /locus_tag="AC16V7_02155"
                     /inference="COORDINATES: protein motif:HMM:NF013089.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HIR6255948.1"
                     /translation="MFRMNLGSFRPLLGSRENLLILILLVINLVFNVVSNAGFRLSAG
                     SATLKDFISWQVIGNLAGFITVLTLTGLLRYIPLHIAFPVTTGLAIIGVQLIAGRWFF
                     GEVISVERWFGTILVVIGILFLTWL"
BASE COUNT          496 a          489 c          462 g          384 t
ORIGIN      
        1 cctgccagcc ccaatacgag agcgccatag cgaggttcgc tgggccgcag ttattgaaag
       61 tctggcgttg gtagcgaatg ccttccaaga tcatcttctc tgggataggg gttggtgtga
      121 cggtcgatgt tggggggggc tccagtgtag ctgtcggctc accaggcagg gctgttgggg
      181 tcaggttggc tatcggattt tgcgcaggca tatctgcacc cgtggtgggc gacgattgga
      241 gcagcgcttg gagggtagcc tgaacgatgg cctccacttg ctcgttttgc tcctttggca
      301 cgaagaccac ctgaccttca ggggctaagg agcggcgcag gtcggcaagc cagttatcca
      361 cccgccaccc gaggcgcgcc tgcacaggcg gcaggaaata tacacccacg cacaacaggc
      421 aggtggtagg caaagcaatt agggagatca ggcagccctg ctttctcatg agcgcgatta
      481 tatcatcggc ggcagatgag gttttctata gatgagatat tattaattcc tccctatggg
      541 tatgcaatat agccaaatct ttcaagaaac gctctcacag acatccaaaa ctcgatgcgc
      601 ttcaatcccc acaagctcct atatcgcaat gcgctggggt tatacgtcca gatcaaaacc
      661 caggagtaga aattctccca gaattcgccg aaagtcccca atgggtgagt ataaggtatc
      721 aacttgaacc ctaaacgctt gagcaagcgg ctcccaccat tctctgtgcc aaggcctgcc
      781 agcaccgtca cacctcccac cgcgctcaca tgagccagtt cagacgactc cttcaggcag
      841 gtagccacca ttcgaaggga gtgcacaaac tggcgttgca tgggcttggc ccatgccaag
      901 tcagggccct ggggagggaa ttggagcaaa cgctcattcc acaagtgcaa taaaagcact
      961 gcatcacccg gagacacgac ccgatctggc aaatccagtt ctatgcgcgc cgttgcaagc
     1021 tgaatgcgca acaggcatcc agggtcctgg ctaaactcga atacaccccg accctgacgc
     1081 acccaggcat caaaattgcg gaccatcgcg cgcaaggtca cagccatgtg agaaacagaa
     1141 tgccaataac caccaggatc gtgccaaacc agcgctcaac cgaaatcacc tcgccaaaga
     1201 accacctgcc tgctatcaat tgaacgccga tgatcgccag acctgtcgtg accggaaagg
     1261 caatatgtaa gggaatatag cgcagcagac cggtcagggt cagtactgtg atgaagccag
     1321 ccagattacc gatcacttgc caactaatga aatctttcag cgttgcgctt cctgcagaga
     1381 gtctaaatcc agcattcgag acgacattga ataccaggtt gatgaccagc aggatcaaaa
     1441 tgagtaaatt ttcgcgggaa cccagaagcg ggcgaaagga gccaagattc atgcggaaca
     1501 tgacacacct ccacacctat acttaattcc aaaaaatcgt taccttccca gctaccttaa
     1561 atctatcact atttcgcggc tgtgacgtag gcttttcagc aaactcactc ttcataatat
     1621 gggattagcg cggaatgatt tatgagagac aagtcatacc aatcacggca caagatggca
     1681 gccaatactt gtgctcgtga gatcgcgaag agaacaagga gcaattcgtg cctggcatgc
     1741 ttttatattt gatagccagc aatagaattc agaataatca agtagaattg gggggtgttc
     1801 tgccagtaca ggaatatact atactattct a
//
LOCUS       DBNHOH010000119         5778 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_133744, whole genome shotgun sequence.
ACCESSION   DBNHOH010000119 DBNHOH010000000
VERSION     DBNHOH010000119.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5778)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_133744"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="AC16V7_02160"
     CDS             complement(<1..551)
                     /locus_tag="AC16V7_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR6255949.1"
                     /translation="MIAPAIPQTQSGRTLVNDEIFVPEIGRVEKIEPLTALEKVFTIE
                     LPQGKSLNHRPGQFLEISALGVGEAPISISSSPSRSNGFVELCVRKVGDVTSALHRLR
                     PGDKVGIRGPFGRGFPIENFRGKDILFAPGGLGLAPLRSLINQVLDERAHFGRVIILY
                     GARAPAELLFKDELRQWEARGDV"
     gene            complement(548..1594)
                     /locus_tag="AC16V7_02165"
     CDS             complement(548..1594)
                     /locus_tag="AC16V7_02165"
                     /inference="COORDINATES: protein motif:HMM:NF028488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR6255950.1"
                     /translation="MSLTIIPKIELSNFINRLLPRYHVAGPVASDGGFAFALINDPDE
                     LQLEYRTTILPPKKYLLPPREILFHFELETNGWVQASYQAHPTVLIGMHTCDLHAFQL
                     LERVFTSGFPDSNYIDRSSQTLIISIECLAPCDAHAFCKSMGTLTADEGYDLHLTDLG
                     DVYAVDIGSKPGEALLKEYARTRTAAPDDISRLNAVLNEKWPRFSYRLDFDVSDLPSL
                     LSVSAKSPIWAQLGERCLGCASCTNVCPTCYCFDVRDEVEMDLQRGQRVRVWDSCQLD
                     EFATVAGGHNFRKSQAMRQRHRFMRKGKYIYDAYGYMGCVGCGRCARACLVEINPVSV
                     FNELYRLREVVQTS"
     gene            complement(1609..2256)
                     /locus_tag="AC16V7_02170"
     CDS             complement(1609..2256)
                     /locus_tag="AC16V7_02170"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013935.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HIR6255951.1"
                     /translation="MRKISCTRMELLALKAQLTLARQGRDLLEEKRTALMKEFLKIAD
                     TILERSDVLQQSAVNARRALARAEAIAGSEAIQSAALVTRSELPLEVTTASVMGVKVP
                     HIEQKRVSRSILQRGYSIVGSSMTIDETASAFEAEVDAIIALAESELRFARLAAEIRR
                     TSRRLNALDHLLIPGLEADLDYIQMALDERERADHFRLKLIKRILERKRIPSNPV"
     gene            complement(2253..3671)
                     /locus_tag="AC16V7_02175"
     CDS             complement(2253..3671)
                     /locus_tag="AC16V7_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HIR6255952.1"
                     /translation="MLKTVPRPRLVTKEYSTLSQIKGPLIFVERIADVAYNEMVEIID
                     PDGKTRLGQVLEVDRQRCMVRIFVGTAGLDLERTHARFKGDVARLDVSLSMLGRVLDG
                     SGQPIDGGPAIIPEASLDINGLPINPSVRTHPNEFIQTGVSAIDGLNTLTRGQKLPIF
                     SGAGLPANELAAQITAQARIAGAKAKGGEPFAVVFAAIGITNREAAFFIDQFRASDAI
                     ERTVLFVNRADDPTIERLLTPRAALTAAEYLAFTHHHHVLAILTDMTNYCEALRETAA
                     ASEEVPGRRGYPGYMYSDLASLHERAGRIHGNPGSVTQLVILTMPDDDITHPIPDLTG
                     YITEGQIVLSRELHRKGIYPPVDVLPSLSRLMNAGIGTGKTRSDHRAVADQLYALYAK
                     GRDLRRLVAIIGEAALSVEDRQVLAFARQFEERFVGQGLIERDIAETLSLAWELLALM
                     PAESLKRIPKEFIEKYHHQKDR"
     gene            complement(3674..5482)
                     /locus_tag="AC16V7_02180"
     CDS             complement(3674..5482)
                     /locus_tag="AC16V7_02180"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004591800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HIR6255953.1"
                     /translation="MNNPLEMGLEDPSGKIIRIAGPVVGAVGLNRVRLYDVVLVGELA
                     LVGEVIRLSGDLAIIQVYEDTSGVRIGEPVVNTGLPLVAQLGPGLLGSVYDGLQRPLK
                     VIAASTGDFIRRGVQAPPLPEEAVWTFTPHISKGDRVGPGDLLGVVPETKTLEHRILV
                     PPGRRGKVIETRSGETRLNDVVVKIESSENHHKELFEITLVQRWPVRQPRPIQTRLPP
                     KSPLISGTRVIDAFFPVAKGGSAIIPGGFGTGKTVVEHSLARWADADVVVYVGCGERG
                     NEMTEVLEEFPVLEDPRTGAPLMERTVLIANTSNMPVAAREASIYTGITIAEYYRDMG
                     YAVLLLADSTSRWGEALREISGRLEEMPGEEGYPAYLAARLAEFYERSGRVICLGGRE
                     SEEKARRIGSVTVVGAVSPPGGDFSEPMTQNSMRVAGTFWALDYSLSRSRHFPAIHWI
                     PSYSLYDLREWYQKQVAPDWADLITEAMALLQREVELLEIVQLVGPDALAETERAVLS
                     AARMLREDFLQQSAYHEIDRYCPIEKAYWMLKLIMEFHQLAQAAIQSNVSLDRITSMP
                     VITEIGRMKELHPEKAEKAIRALQDRVHYSFAELGV"
     gene            complement(5466..>5778)
                     /locus_tag="AC16V7_02185"
     CDS             complement(5466..>5778)
                     /locus_tag="AC16V7_02185"
                     /inference="COORDINATES: protein motif:HMM:NF014092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HIR6255954.1"
                     /translation="VDGFRLAGVEAFAAEDVETAQELIESWVEAGESGLVAIDDAYLE
                     HINPMVIRRLEASDQLDYLAIPGGEPLGPETSRRQRLAQQIRRAIGFHITFKGEDEQS
                     S"
BASE COUNT         1376 a         1682 c         1472 g         1248 t
ORIGIN      
        1 tccacatctc cccgcgcctc ccactggcgt agctcatcct tgaagagcag ctccgctggg
       61 gcgcgcgccc cgtacaggat gatgacccgg ccgaaatgag cacgctcatc aagcacctgg
      121 ttgatcaacg agcgaagcgg tgctaagccc aagcccccag gcgcgaacaa tatatctttt
      181 ccgcggaaat tctctattgg gaagccacgt ccaaacggtc cgcgaatacc caccttgtcg
      241 cctggtctca ggcgatgcag cgcgctagtt acgtctccca ccttgcgcac acataattca
      301 acaaatccat tgctccgact gggtgagctg ctgatgctga taggggcttc gccaaccccc
      361 aaggcagaga tctccaagaa ctgtccggga cggtggttca gggatttgcc ctgggggagc
      421 tctatggtaa agactttctc cagcgcggtc aggggctcaa tcttttctac ccggccaatt
      481 tccggaacga agatctcatc attgaccaat gtgcgtccag attgtgtttg gggaatagct
      541 ggtgcgatca tgatgtttgc acgacctctc ttagacggta aagctcatta aatacactca
      601 ccgggttaat ttccaccagg catgctcgcg cgcaacgtcc acacccaaca caacccatat
      661 atccgtatgc atcataaata tatttaccct tgcgcataaa gcggtgtcgc tggcgcatcg
      721 cctggctttt gcgaaagttg tgtccacctg ccacagtagc aaattcatca agctggcaag
      781 aatcccatac tctcaccctt tgcccgcgct gcaggtccat ttccacttcg tcacgcacat
      841 cgaagcaata acacgtgggg cagacattgg tgcaggatgc acaccccaag cagcgctcac
      901 ctagctgagc ccagatggga ctcttggcgc tgacactaag caaagaaggg agatcgctta
      961 catcgaaatc caagcggtag gaaaagcgag gccatttttc attgagcact gcgttcagac
     1021 gggagatatc gtccggtgct gctgtacggg ttcgagcata ttcctttaac aacgcttcgc
     1081 caggctttga gccaatatcc actgcgtaaa catcccccag gtcggtaagg tgcaggtcat
     1141 agccctcgtc ggcagtaagc gtccccatgc tcttgcagaa ggcatgcgca tcgcatgggg
     1201 ccagacattc aatactgata ataagcgttt gactgctgcg atcgatgtaa tttgaatctg
     1261 gaaaccccga agtaaaaacc cgttcaagca attggaaggc gtgtaaatcg caagtatgca
     1321 tgccaatcag cacggtaggg tgcgcctgat atgaggcctg aacccagccg ttggtctcca
     1381 actcaaagtg gaaaagaatt tcgcgcggcg gaagcaggta cttcttgggc ggcaggatcg
     1441 tcgtgcggta ttccagctgt aactcgtcag ggtcattgat caatgcaaag gcgaaaccac
     1501 catcgctggc aactggtcca gcgacgtgat aacgtggcaa cagacgatta atgaaattcg
     1561 aaagctcaat tttaggaatg atggtcaggc tcacggttcg cttctccatt aaacaggatt
     1621 gctaggtatg cgcttccttt cgagaatcct tttaatcagt ttgagacgaa aatgatcggc
     1681 gcgctcgcgc tcgtccagag ccatctggat ataatccagg tcagcctcca acccgggaat
     1741 caacagatga tccaaagcat tcaggcggcg agacgtacgg cgaatttcgg cagccaggcg
     1801 agcgaagcgc agctcacttt cagcaagggc aatgatggca tccacttccg cctcaaaagc
     1861 ggaggcggtc tcatcgatcg tcatcgaaga accaacgatg gagtagcccc gctgcaggat
     1921 cgagcgagag acacgttttt gctcgatatg aggcaccttg acgcccatca cacttgctgt
     1981 agtcacctcc aggggtagct cagatcgggt gaccagcgca gccgattgga tcgcctcgga
     2041 cccagcgatg gcctccgcac gagccagtgc acgccgggca ttcactgctg attgttgcaa
     2101 tacatcagaa cgttccaaga tcgtatcagc gatcttcaaa aactccttca tcagggctgt
     2161 acgcttctct tccaagagat cccgcccctg gcgcgcaagc gtaagttgcg ctttaagggc
     2221 gagcagctcc attcgagtgc aggatatttt tctcatcgat ctttctgatg gtggtacttc
     2281 tcaatgaatt ctttggggat ccgcttcaat gactcagcag gcatgagtgc cagcagttcc
     2341 caagccaggc tcaacgtctc ggctatatct cgctcaatta aaccttgacc gacgaatcgc
     2401 tcctcaaatt gccgcgcaaa agcaagcacc tggcgatcct cgacagagag agctgcctca
     2461 ccaatgatgg ctactaagcg acgcaaatca cgaccctttg cgtacagtgc ataaagctga
     2521 tctgcgacag cccggtgatc ggagcgcgtc tttcctgttc ctataccagc gttcatcaat
     2581 cgcgaaagcg agggcaggac atcaacaggt gggtaaatcc ctttccggtg caactcgcgg
     2641 ctcaagacga tctgtccctc agtaatgtaa ccggtcaaat ccggaatcgg atgagtgata
     2701 tcgtcatctg gcatcgtcag gataaccaat tgcgtcacgg aacccgggtt gccgtggatt
     2761 cgcccagcgc gctcatgcag gctagccagg tcggagtaca tataaccggg ataaccgcgc
     2821 cgaccaggaa cctcctcgct ggctgcagcg gtctcgcgca aagcctcgca gtagttggtc
     2881 atatcggtca ggatagccag tacgtgatgg tgatgggtga aggccaagta ttcggcagcg
     2941 gttaaagcgg cgcgcggcgt cagaaggcgc tcaatggtgg gatcatcggc tcgattgacg
     3001 aacaacacag tgcgctcaat cgcatcgctg gcccgaaatt gatctatgaa gaaggcagcc
     3061 tcgcgattgg taatgccgat agccgcaaaa accaccgcaa acggttcacc ccctttggct
     3121 tttgcacccg caatgcgcgc ttgggctgtg atctgtgcgg cgagctcatt ggcaggcaag
     3181 cctgccccag agaatatggg caatttctga cctcgagtta gagtgttcag tccatcgatt
     3241 gcggagacgc cggtttgaat gaactcattg ggatgggttc gcacagatgg attaatgggc
     3301 aacccattga tgtccagtga tgcctcaggg atgatagctg gaccgccatc gataggctgg
     3361 cccgagccat ccagaacgcg cccaagcatg gataaagaga catctagccg ggcaacatcc
     3421 cctttgaaac gggcatgggt gcgctccagg tccagcccag ccgtgcctac gaagatgcgc
     3481 accatgcagc gctgacgatc tacctcaagc acttgcccca ggcgggtttt accatcggga
     3541 tctatgattt ccaccatctc gttgtacgcc acatcagcga tacgctcgac aaagatcagc
     3601 ggacccttaa tctgtgaaag cgtactgtat tctttggtaa ccagcctggg tcgaggtacg
     3661 gttttcagca tttttacaca cccagttcag caaagctata gtgcacgcga tcctgcaacg
     3721 ctcgaatggc tttttcggcc ttctcagggt gcagctcctt catgcgccca atctccgtga
     3781 taacaggcat gctggtgatc ctatcgagtg aaacatttga ttgaatggct gcttgcgcca
     3841 gttgatggaa ttccataatg agcttgagca tccagtaggc tttttcgata gggcaatagc
     3901 ggtcgatctc atgataagct gactgttgca aaaaatcctc gcgcaacatg cgggcagcac
     3961 tgagtaccgc ccgctcggtt tcagcaagcg catctggtcc aaccaattgg acaatctcaa
     4021 gcagctccac ttcacgctgg agcagtgcca tagcctcagt gatcaggtct gcccaatctg
     4081 gcgcaacttg tttttggtac cattcccgca gatcatacaa ggaataactg ggtatccagt
     4141 gaatggctgg gaaatggcgg ctgcgcgaaa ggctataatc caatgcccag aaggtgccag
     4201 caacccgcat cgaattctgg gtcatcggtt cggagaagtc accacctggc ggggagactg
     4261 ctccaaccac tgtcactgag ccaatccgcc ttgctttctc ctcactctcg cgccccccga
     4321 ggcagatgac acgtccagag cgctcgtaga attctgccaa acgcgctgcc agatacgccg
     4381 gatagccctc ctcgcccggc atttcctcca atctcccaga gatctcgcgt aaagcctcgc
     4441 cccagcgaga cgttgagtca gccagtaaga gcacagcata ccccatgtct cggtaatact
     4501 cagcaatcgt aataccggta taaatgctgg cttcacgtgc tgcaacgggc atattggagg
     4561 tgttcgcaat gagcaccgtg cgctccatca gaggcgctcc agtgcgcggg tcttccaaaa
     4621 cagggaactc ctccaagacc tcggtcatct cgttaccccg ctcgccgcag cccacgtaga
     4681 ccaccacatc ggcgtccgcc catcgagcca ggctatgttc caccacagtc ttacccgtgc
     4741 caaaaccccc agggataatt gccgaaccac ctttggctac cgggaaaaag gcatcgataa
     4801 cacgcgtgcc gctgatgagt ggtgacttgg ggggcaagcg ggtttgaatc ggacggggct
     4861 gccggacagg ccagcgctgg acaagcgtga tctcaaaaag ctctttatgg tgattttcag
     4921 aagattcaat cttgaccacg acatcgttca atctcgtttc accgctgcgc gtctcaatta
     4981 ccttccctcg ccgacctggt gggactaaaa tgcgatgctc tagggttttc gtttcaggca
     5041 cgacccccaa caaatctcca ggtccaaccc gatccccttt cgatatgtgc ggtgtgaagg
     5101 tccatacagc ctcttctggc aaaggcggag cctgaacccc gcgccggatg aaatcgcctg
     5161 tggaggctgc aataaccttc aacggacgtt gcagtccatc gtatacgctc ccaagcagcc
     5221 ctggaccaag ctgtgctacc aagggtagac cagtattgac cactggctcg ccaatgcgca
     5281 cacccgatgt gtcctcataa acctggatga tcgccagatc acccgagagg cggatcacct
     5341 cgcccactag tgccaattcc cccacaagga cgacatcata caaacggact cggtttaatc
     5401 ccacagcccc caccaccggg ccggcaatgc ggataatttt tccggatgga tcttccaatc
     5461 ccatttcaag aggattgttc atcttctccc ttaaacgtaa tgtggaagcc gatcgcccgt
     5521 cgaatttgct gggccaagcg ctgccgccgc gaggtttccg gtccgagagg ctctccaccg
     5581 ggtatggcga gatagtccag ttgatcagaa gcctccagac gcctgatcac catgggattg
     5641 atgtgctcga gataggcatc atcaattgcc actaatccag attcacccgc ctccacccag
     5701 ctctcaatga gctcctgagc ggtctctaca tcctctgcgg caaaagcctc aacccctgcc
     5761 aaccggaagc catcgacc
//
LOCUS       DBNHOH010000120         3195 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_133830, whole genome shotgun sequence.
ACCESSION   DBNHOH010000120 DBNHOH010000000
VERSION     DBNHOH010000120.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_133830"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..1536
                     /locus_tag="AC16V7_02190"
     CDS             118..1536
                     /locus_tag="AC16V7_02190"
                     /inference="COORDINATES: protein motif:HMM:NF018012.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR6255955.1"
                     /translation="MIKLNVLTENEIEAIHESSLRILAETGIVLTHPQGREILAAAGA
                     IVESDRVLLPPELVEEAIARCPNEVRLRGRGGMVKVLGDGSLRWHNLGGARDVYEPVS
                     GTRRKALLQDLRDSTRLLDALDQVDSITPFFTPQDVPGAIMSLAMYRYALPHTTKVLQ
                     GPGVQSAAEVRYTARMAAVVGPPEEVVSLSVSPVSPLGFPNDAVEAILETARLNITFG
                     PLPCPTAGATAPLSLAGALAQQNAEVLAAVVLAQLAQPGLPIVYCGRLAMLEPRTGLS
                     VWGGVELGLASAATVQLGHRYGLPVNVYGFSTNSYTLDIQNGFERALNAAIPALAGAD
                     ELSGIGEMGAGVSSCFAQMVCDNELAASVGRLIRGFSSDDDARAVDVIAAVMRGSRNF
                     LGQKHTSRYLRAGEVYMTRLAERGSWDTWEAGGGQGLAERAQAEAERLLREHQVPPLS
                     DDQERELDAILDEAQQVLISRI"
     gene            1549..2733
                     /locus_tag="AC16V7_02195"
     CDS             1549..2733
                     /locus_tag="AC16V7_02195"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 metallopeptidase family protein"
                     /protein_id="HIR6255956.1"
                     /translation="MENMLSNAKRYESQLVDWRRDIHQHPELGFEEHRTAARVAEVLA
                     SLGWRVRSGVGRTGVVGELGHGSPIVAIRADMDALPIIEADDRPYASLKPGLMHACGH
                     DAHVAIALGVAALLKDYPFPGTVRLFFQPSEEVADDEGISGAPRMIADGAMQDVDMVL
                     ALHVDAHSPVGVIRIGSGPSSGGEDSFFGIITGKGGHGARPHETVDPFYLTAHVILAL
                     NGIISRRLDPFHPAVVSIGRVQGGDAENVIPDQVELSGTIRFMERPVRKQLHEEIRRA
                     FEIARTLGGDYTLTFENGGLPMINDPKAVNLITSAATSLIGSEKVLPPIASLGAEDFG
                     SFTEIAPGAMFSLGARIEGDERHHHNPRFDIDEGCLPIGAAILAETALRYLHQSNGRP
                     AG"
     gene            2827..>3195
                     /locus_tag="AC16V7_02200"
     CDS             2827..>3195
                     /locus_tag="AC16V7_02200"
                     /inference="COORDINATES: protein motif:HMM:NF018012.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR6255957.1"
                     /translation="MNTLPHVQPRLSLLSPEQIEVVHTKSLDILARVGLRVDSGKAAE
                     ILAKADGVKFITPDHAVIQPKLVEWAIQSAPSTIQVYNRRGEPAFQLGADRTRFGVGV
                     TNLYYQDPLSDQISPFNRELL"
BASE COUNT          665 a          920 c          959 g          651 t
ORIGIN      
        1 cctgtagatt tccatctcta tcctgacgaa gggcacggct tcctcaagac agagaatgtc
       61 atcgacgcgg ctcggcgcag ggtacaattt ttagccagag cattggaata gaaagctatg
      121 atcaaactga atgtgcttac cgaaaacgaa attgaagcga tccacgaatc cagcctgcgc
      181 atccttgccg agaccggtat cgtgcttacc cacccgcaag ggcgtgagat attggctgcg
      241 gctggagcta tagtggaaag cgaccgtgtc ttgctcccac ccgagcttgt cgaagaagcc
      301 atcgcccgct gcccgaacga agtgcgcttg cgggggcgag gtgggatggt caaagtactg
      361 ggagatggca gccttcgctg gcataacctt ggcggtgcgc gcgatgtcta tgaacctgta
      421 agtgggactc ggcgtaaggc cttattgcag gatctgcgcg acagcacgcg tttgctggat
      481 gcactggacc aggtggacag catcaccccg ttcttcactc cccaggatgt acccggggca
      541 atcatgtcgc tggcgatgta tcgctatgca ctcccccaca ctactaaggt cttgcaagga
      601 ccaggtgttc aaagcgctgc cgaagtgcgc tacacggctc gcatggcagc cgtcgttgga
      661 ccacccgaag aggtcgtctc gctcagtgtc tcaccggtta gcccattagg ctttcccaac
      721 gacgcagtgg aggccatcct cgagacggcg aggctgaata tcacctttgg tccactcccc
      781 tgccctaccg ctggggctac cgcccccctc tcgcttgctg gggctctggc gcagcagaac
      841 gccgaggtgt tagccgccgt tgtactagca caacttgccc aacccgggct acccatcgtc
      901 tactgcggcc ggctggcaat gctggagccg cgcaccgggc tctcggtctg gggcggtgtg
      961 gagctgggat tggcctcggc agccacggta cagctggggc accgctacgg tttgccggtc
     1021 aatgtatacg gcttctcaac caactcgtat acgctggata ttcagaatgg tttcgagcgg
     1081 gcactgaacg cggccatacc ggctctggct ggcgctgacg agctttccgg aatcggcgag
     1141 atgggggctg gggtgagcag ctgttttgcc caaatggtct gcgataatga gctggctgcc
     1201 agcgtgggcc gcttaatacg tggctttagc agcgatgatg atgcccgcgc cgtggacgtc
     1261 attgcagcgg tgatgcgtgg atcgcgcaac ttcctgggac aaaaacatac cagtcgctat
     1321 ctgcgcgcag gcgaagtata tatgacccgt ctggcggagc gcggttcctg ggatacctgg
     1381 gaagctggtg gagggcaggg cctagctgag cgtgcccagg ccgaagctga gcgtctgctt
     1441 agagaacacc aagtgccccc gctaagcgat gatcaggagc gagagctgga tgctatactt
     1501 gacgaagccc aacaggtttt gatctccagg atttaaggtt aaagctaaat ggaaaacatg
     1561 cttagtaatg cgaagagata tgaaagccaa ctggtcgact ggcggcgcga catccatcag
     1621 catcccgagc tgggttttga ggagcatcgc accgctgccc gagtcgctga agtcctcgcc
     1681 agcttgggct ggcgcgtgcg cagcggggtc gggcgcacag gggtggttgg cgagctaggt
     1741 catggttcgc ccatcgtcgc catccgcgct gacatggacg ccttgccgat catagaggca
     1801 gatgaccgcc catatgcttc cctaaaacct ggattgatgc atgcctgcgg tcacgacgca
     1861 catgtcgcca ttgccctagg ggtggctgct ttgctaaaag attatccctt tccagggaca
     1921 gtgcgcttat ttttccagcc ttccgaagaa gttgcggatg acgaagggat aagcggcgcg
     1981 ccgcgcatga tcgccgatgg cgccatgcaa gacgtagata tggtgctggc gttgcacgtg
     2041 gacgcccaca gtccggttgg agtgatccgc ataggctcgg gaccctcttc tgggggtgag
     2101 gatagcttct tcgggataat cactggcaaa ggcgggcatg gcgcccgtcc gcacgaaacg
     2161 gtggatccct tttatttaac cgcgcatgtc atcctggcac tgaacggcat catctcgcgg
     2221 cgtctcgacc cgttccatcc ggcagtagta agcatcggga gggtgcaagg cggtgatgcg
     2281 gaaaacgtta tccccgacca ggtggagctc tcaggtacca tccgttttat ggaacgccct
     2341 gtccggaagc agttacacga agaaatccgc cgcgctttcg agatcgcgcg cactttgggc
     2401 ggtgattaca ctttgacttt cgaaaacggc ggcttgccaa tgatcaacga tccgaaggcg
     2461 gtcaatttga tcacaagcgc cgcaaccagc ttgatcggga gcgagaaggt gctcccaccc
     2521 atcgccagtt tgggcgctga ggactttggc tcttttacgg agatcgcccc cggggcaatg
     2581 ttctcactgg gtgcccgcat tgagggtgac gagcgccacc accataaccc gcgctttgac
     2641 atcgacgagg gctgcctgcc cattggggca gccatcctgg ctgagacagc gctccgctat
     2701 ctccaccaga gtaatggacg tcccgcaggt tgattatcac gcatgctcgt tttagggaaa
     2761 cctgcgggac gttattacac ctgagagctc aaccgatttc ggtgaacacc catgggaagg
     2821 aaaaagatga acacactccc ccacgtccaa ccccggctca gcttactatc tccagagcag
     2881 atcgaggtgg tgcataccaa atccctggat attctggcga gggtcgggtt gcgcgttgat
     2941 tcaggcaaag cggccgagat cctggccaaa gcggatgggg tcaaattcat cacccctgac
     3001 cacgccgtga tccaacccaa gctggtggag tgggctatcc aaagcgcgcc atccaccatc
     3061 caggtctaca accgccgcgg agagccggcc ttccagctgg gcgcagaccg cacccgcttt
     3121 ggcgtggggg tgaccaacct gtactatcag gacccgctca gcgaccagat cagccctttc
     3181 aaccgcgaac tgctg
//
LOCUS       DBNHOH010000121         3506 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_6282, whole genome shotgun sequence.
ACCESSION   DBNHOH010000121 DBNHOH010000000
VERSION     DBNHOH010000121.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_6282"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="AC16V7_02205"
     CDS             complement(<1..178)
                     /locus_tag="AC16V7_02205"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF014873.7"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR6255958.1"
                     /translation="MALAEKTMTKGRVVQILGGVVDVEFPPEQLPEIFEAITIPRDGQ
                     NSLVLEVQKHLGKNW"
     gene            complement(204..1103)
                     /gene="atpG"
                     /locus_tag="AC16V7_02210"
     CDS             complement(204..1103)
                     /gene="atpG"
                     /locus_tag="AC16V7_02210"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HIR6255959.1"
                     /translation="MPNTREVRLRIRSIKNIAQVTRALQAVSASKVRKAMQAVMNTRP
                     YATKAWQVLTHIAGQPGRESLHPLLTNRPEVKSVLVLMITGDRGLAGAYNTNVVRFVL
                     HRFNDYPVPVRYITIGRKGRDLMLRRRKDVIAEFSNLPAAPAFADISAIGRIAVEEFL
                     HLHSDEVYLIYTDFISMARQVATIKKLLPLEVDTGEGRVMTFSQSARGPAAAYIYEPG
                     EAGIIDQIVPRFTALQVYQAILESLASEHAARMVAMKNATDSAKELAGALQLQYNKTR
                     QQAITNEMLDIAGGAEALESLRE"
     gene            complement(1166..2827)
                     /gene="atpA"
                     /locus_tag="AC16V7_02215"
     CDS             complement(1166..2827)
                     /gene="atpA"
                     /locus_tag="AC16V7_02215"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR6255960.1"
                     /translation="MSDLISQIAADLKKQIESFQPELEVRDVGSVIEAGDGIARVRGL
                     GDVRAQELVQFDNGVLGIAFNLESDQVGVIIMGEYAGIDEGMLVRSTGRIASVPVGDG
                     LVGRVVNALGQPIDGKGPIKYESFRPIERIAPGVVARKDVDTPVQTGLKAIDSMIPIG
                     RGQRELIIGDRQTGKTALAIDTIINQKGKDLICIYVAIGQKKAAIARTVATLERFGAM
                     DYTTIVVSAADEPAALQYIAPYAGCAMGEEFMETGRDALVVYDDLSKHAWAYRQVSLL
                     LRRPPGREAYPGDVFYLHSRLLERAARLADNFIIVKASHTDEVGDIKDSVNDTVYEGP
                     LAQHNAEAALKSMENADANKMVKLRGSGGSLTALPIIETLLGDVSAYIPTNVISITDG
                     QIYLENNLFYAGIRPAVNVGISVSRVGGDAQTRGMKQVAGRLRLDMASFRELAAFAQF
                     GSDLDKSTQAQLNRGQHLQEILKQPQYEPMSMESQVMVIFAGTQGYADKVSLERMRAW
                     ETSLLYFLETSYPEIGKDIAEKKRITDENMEKLRSALDAFNSTWQ"
     gene            complement(3025..>3506)
                     /locus_tag="AC16V7_02220"
     CDS             complement(3025..>3506)
                     /locus_tag="AC16V7_02220"
                     /inference="COORDINATES: protein motif:HMM:NF014068.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HIR6255961.1"
                     /translation="EANYRIVIASNTLEQLEAMYIQHGFESSAPISRTLPLITADLLL
                     GDVQISDYDAFILISDEALVFKGGEEVKQIIREAIEQGKVVAAHDAGVLILGNAGILQ
                     GVEITSNPLICMQLEEEDGAICRQRTVHRDGQIITAGPDHASSSFVKQIIAAIQDSN"
BASE COUNT          842 a          962 c          800 g          902 t
ORIGIN      
        1 cccagttttt ccccaggtgc ttctgcacct ccaaaacaag ggagttttgg ccgtcacgcg
       61 ggattgtgat cgcttcaaaa atttccggga gttgttcggg cggaaattcc acatccacca
      121 caccacctaa aatttgaacg actctaccct tggtcattgt tttttctgct aaagccatag
      181 cgcctatctc cgttctctat tcattattct ctcaggcttt ccaatgcttc cgcaccacca
      241 gcaatatcca gcatttcatt ggtaatcgcc tgttggcgtg ttttgttata ttgcaattga
      301 agcgcacccg ccagctcttt ggcgctgtcg gtagcatttt tcatcgccac catgcgggct
      361 gcatgctcgc tagccaggga ttctaaaatc gcctgatata cctgcaaagc tgtaaatctc
      421 ggcacaatct ggtctataat gcctgcctcg cccggctcat aaatatatgc tgcagctggt
      481 ccgcgcgcag attgggaaaa ggtcattacc cgtccttctc cggtatccac ttcaaggggc
      541 agcaatttct taatcgttgc tacttgccga gccatactga tgaagtcggt atagatcagg
      601 tacacctcgt cactgtgcag atgaagaaac tcctcaactg ctatccggcc aattgcggat
      661 atatccgcaa acgcaggagc tgccggcaaa ttgctaaatt cggcgattac gtctttgcgc
      721 cgacggagca tcaaatcacg gcctttacgt ccaatggtga tatagcggac tggtacagga
      781 taatcattga aacggtggag tacaaatcgt acgacgttgg tattgtatgc acctgccaga
      841 ccgcgatcac ctgtaatcat caacaccaag acgctcttta cctcagggcg attggttaat
      901 aacggatgca gggattcgcg cccaggctgc cctgcaatat gcgttaatac ctgccaagcc
      961 ttggttgcat aagggcgcgt gttcatcact gcctgcatcg ccttgcggac tttactggcg
     1021 ctcaccgcct gcaaagcccg ggttacttgg gcaatattct ttatgctccg gatgcggagc
     1081 cggacctcac gagtattagg catagatttc taccgagatc gttatatcca ctctattaat
     1141 cgtctgaacc agaaccttca atcaattatt gccaagtgct gttgaaggca tccaacgcac
     1201 tgcgcaactt ctccatgttt tcgtcggtga tacgcttctt ctcggcaata tccttgccta
     1261 tctcaggata ggaagtctcc aagaaatata aaagcgaggt ctcccaggca cgcatgcgct
     1321 ctaacgacac tttatctgcg tagccctgtg tacccgcaaa gatgaccatc acctggcttt
     1381 ccatcgacat cggctcgtac tgaggttgtt tgaggatttc ctgcaagtgc tgtccacgat
     1441 ttaactgtgc ctgggtggat ttatccaggt ctgatccgaa ctgagcaaaa gccgccagct
     1501 cgcgaaatga agccatatcc agtcgcaggc gccctgcgac ctgcttcata ccgcgtgtct
     1561 gggcatcacc acctacgcgg gataccgaaa tacccacatt cacagccgga cgtataccgg
     1621 cgtaaaacag gttattctcc aggtagatct ggccatccgt aattgaaatt acattggtcg
     1681 ggatatatgc cgaaacatcg cccaggaggg tctcaatgat gggaagggct gtcaatgagc
     1741 cgcctgagcc acgcaatttg accatcttat ttgcgtctgc attttccatg cttttcaagg
     1801 cagcttcggc attgtgctgt gcaagaggac cctcgtagac agtatcattg acgctgtcct
     1861 tgatatcacc tacttcatca gtgtgactgg cttttactat aatgaaattg tcggcaagac
     1921 gggcagcgcg ttccagcaag cgagagtgca ggtaaaacac atcacctggg taggcctcgc
     1981 gcccgggcgg gcggcgcagt aagagcgaaa cttgccggta agcccaggca tgtttagaga
     2041 ggtcatcgta gacaaccaga gcatcacgcc cggtttccat gaattcctca cccatggcgc
     2101 atccagcgta aggggcaata tactgtagag ctgctggttc gtcggcagcg gaaactacga
     2161 tcgtggtgta gtccatcgcc ccaaaccgct caagtgtggc gaccgtgcgg gcgatggcgg
     2221 ctttcttctg tccaatggca acatagatac agatcaagtc cttgcctttt tgattgatga
     2281 tcgtatcaat tgctagggcg gttttcccag tttgccgatc gccgatgatc agctcgcgct
     2341 gaccgcgtcc gatggggatc attgagtcta tagcctttaa accggtctgg acaggcgtat
     2401 caacgtcttt tcgagctacc acaccgggtg cgatgcgctc aatgggacga aagctctcat
     2461 actttatcgg gcctttgcca tcaatcggtt gacccaaggc attaaccacc cgccctacca
     2521 ggccatcacc tacgggcacc gatgcaatgc gccctgtcga gcgcaccagc atcccttcat
     2581 cgataccagc gtattcgccc atgatgatga cacccacctg gtcactttcc aagttaaatg
     2641 cgattcccaa aacgccgttg tcaaactgaa ccaactcctg ggcgcgcacg tcacccaaac
     2701 cgcgcacccg ggcgatgccg tctccggctt cgatcaccga gccgacatca cgtacctcta
     2761 attcaggttg gaaggattca atttgttttt ttaggtcagc ggctatttgg ctgattaaat
     2821 cggacatttc gcctccgcag aatataatga ttatacatac cgccgcccgg ataaatttaa
     2881 catccgggca gctattggtt tctgataatg gatttcgtca gaccacaacc catgtagaaa
     2941 taattcctga tcattaaagt ggtcaaatga tacctgaaac agcccgactt gtccagttat
     3001 tcacaaaaat atatttggct cgccctagtt gctgtcttga atagctgcaa ttatctgctt
     3061 cacaaaactt gagcttgcgt gatccggccc ggcagtgatg atttgtccat cccgatgtac
     3121 agtcctttgc ctacagatcg cgccatcctc ttcttccagc tgcatacaga taagtggatt
     3181 agaagtgatc tcgactccct ggagaatgcc agcattgcct aaaatcaaaa cacctgcatc
     3241 gtgcgcggcc acaacttttc cttgctcaat cgcttcacgt ataatttgtt tgacctcttc
     3301 accacccttg aataccaatg cttcatcact gatcaatata aatgcatcat agtcactaat
     3361 ctgaacatcc cccagcaaaa gatcagcggt tattaacggt aaagtgcgtg aaatcggtgc
     3421 cgaagattca aatccatgct ggatatacat tgcttcgagc tgctcgagcg tgttagaggc
     3481 aatcacaatc cggtaattgg cttcct
//
LOCUS       DBNHOH010000122         1423 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_6683, whole genome shotgun sequence.
ACCESSION   DBNHOH010000122 DBNHOH010000000
VERSION     DBNHOH010000122.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_6683"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1366
                     /locus_tag="AC16V7_02225"
     CDS             <1..1366
                     /locus_tag="AC16V7_02225"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR6255962.1"
                     /translation="YLWTQEDGLGKPLHGVPIETRADGQKVPLSYITSTCFTPDDRGL
                     IFVTALHEDTRGLGYLTFEHPEDVLRVDITGIMHQGIGELESLEEIGGDRYLLTFNIN
                     GSSWLYEGSFDENNLTMKLNNVLCGTGQISNGVLESVDYDRHQDRFALAFSTATSPTQ
                     IYTIEGKDRRTILRQTNERILAIPADYLSSGENVSYTSFDGTPISARLYMPSPALGFQ
                     GPRPIILYVHGGPQSQERPDFAWFSIPLIQFLTLNGFAVFVPNVRGSTGYGLSFTKQV
                     DKDWGGMDRLDHVHAMTKILPEDNRLDVHRAGVVGRSYGGYMTLTLAGRHPELWSAAV
                     DMFGPYDLITFSERIPETWKPYFKLVLGDAQNETDKRFLIDRSPRTHLENLACPMLVI
                     QGKNDPRVVEHESSDLVAELRAKGKDISYLVFENEGHDVLKYENRVKCYNAITDFFKN
                     YLKP"
BASE COUNT          395 a          346 c          318 g          364 t
ORIGIN      
        1 ctatttatgg acacaagaag atggtcttgg taagccttta catggtgttc ccatcgaaac
       61 tcgagccgat ggacagaaag ttccattaag ttacattacc tccacatgct ttacccctga
      121 tgatcgcggt ctcatcttcg ttaccgcgct gcatgaagat acacgcggac tggggtattt
      181 gacctttgag catcctgaag acgttctacg agtggatatc acagggatta tgcatcaagg
      241 aatcggcgag ctagaaagcc ttgaagaaat tggaggtgat cgctacttgt tgaccttcaa
      301 tattaacggt tcttcatggc tatacgaagg aagctttgat gaaaacaacc taaccatgaa
      361 gttaaataac gttctttgcg gaacagggca aatctccaat ggagttcttg agtccgttga
      421 ttatgatcgc caccaggatc gattcgccct agcattctct acagctacca gtccaactca
      481 aatctacacc atcgagggca aagatcgcag aaccatttta cggcaaacaa acgagcgtat
      541 attagctata ccagcggact atctctccag cggggagaat gtttcgtaca cttccttcga
      601 tggcacgcct atctctgcca ggctttacat gccatctcca gcacttggct ttcaaggacc
      661 taggccaatt attttatacg tccatggcgg tccacaaagc caggagcgcc ctgattttgc
      721 ctggttctca attccattga tccaattcct taccttgaac gggtttgcag tgtttgtacc
      781 aaatgtgcgc ggcagcacag gctatggatt gagctttacc aaacaagtgg ataaggattg
      841 gggaggcatg gaccgtcttg atcacgtcca cgcaatgaca aaaattctcc cagaagataa
      901 tcgcctggac gtgcaccggg cgggcgtcgt agggcgatcg tatggtggct atatgaccct
      961 cactttagct ggacgccacc ctgaattatg gtctgctgca gtggatatgt tcggcccgta
     1021 tgatctgatc acattttcgg agcgaatccc agaaacctgg aaaccctact ttaaattagt
     1081 cttaggcgat gcacaaaacg aaaccgataa gcgtttcctt attgaccgct cgcctcgtac
     1141 acacctagag aatcttgctt gtccaatgct ggtcattcaa ggcaaaaacg acccccgcgt
     1201 tgttgagcat gaatccagcg atcttgttgc agaactgcga gcaaaaggga aagatatctc
     1261 atatttggtg ttcgaaaatg aaggtcacga tgtcctcaaa tatgagaatc gggtcaaatg
     1321 ctacaatgcg ataactgatt tctttaagaa ctatctcaaa ccgtgaataa cctaaaaaca
     1381 caatctggcg accttgtcca actagtcagc ccaaccaaaa aag
//
LOCUS       DBNHOH010000123         1276 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_6730, whole genome shotgun sequence.
ACCESSION   DBNHOH010000123 DBNHOH010000000
VERSION     DBNHOH010000123.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_6730"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1217
                     /gene="murD"
                     /locus_tag="AC16V7_02230"
     CDS             <1..1217
                     /gene="murD"
                     /locus_tag="AC16V7_02230"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR6255963.1"
                     /translation="SVRWVTGGHPVELLNDADLVCVSGGVPLTNPIVVEAQARGIPLS
                     NDSQIFLEVTPCQVIGITGSAGKTTTTSLVGKIAVERVTESVKLQASRRVYVGGNIGL
                     PLIARVDEMAPEDIVIMELSSFQLEIMNRSPQIAAVLNVTPNHLDRHETMAAYVAAKR
                     RILEYQGVEDVAVLGRDDPGAWGMAGMVMGGLVSFGFGGLESGVGTYIRGNDVLYAGD
                     DSEVWIMDISSVQLRGEHNMQNVLAACAIAAAAGIPPAAMRAGVSGFTGVMHRLEFVR
                     RWGGADWYNDSIATAPERTIAAIRSFEEPIILLAGGRDKDLPWGDFAALVSHRVDHLV
                     LFGEAAEKIGLAMGNYPYTRCANLKEAVQAAVHLVEPGDVVLLSPGGTSFDEFKDFEE
                     RGECFKQLVMSL"
BASE COUNT          283 a          293 c          395 g          305 t
ORIGIN      
        1 agtctgttcg ctgggtgacg ggggggcacc cggtagagtt actcaatgat gctgatctgg
       61 tttgtgtttc agggggtgta cccctgacga acccgattgt tgttgaagca caggcgagag
      121 gtattccgct ctcgaatgac tcgcaaatat ttcttgaggt gactccatgc caggtgatcg
      181 gtatcaccgg ttcagcaggt aagacaacaa cgacatcatt ggttggaaag atcgccgtgg
      241 agagagtcac ggagtcagtt aaattacagg cctcaagacg tgtttacgtc ggcggtaata
      301 tcggtttacc tctcatcgct cgggtggacg aaatggcacc tgaggacata gtgataatgg
      361 aattatcgag ttttcaattg gagattatga atcgctctcc gcaaatcgcg gctgtgctaa
      421 atgtaacccc taaccacctc gaccgccatg agacaatggc agcctacgtg gctgcaaaaa
      481 gacgtatcct cgagtatcaa ggcgttgaag acgtcgcggt ccttggtcgc gatgatccgg
      541 gagcttgggg gatggctggc atggtgatgg ggggcttggt atcctttggt tttggtgggc
      601 tagaaagcgg cgtgggtacc tatattcgcg gaaacgatgt gctctatgca ggagatgatt
      661 ctgaagtctg gatcatggat attagtagcg tacaactgcg aggcgagcac aatatgcaga
      721 acgtattggc tgcctgtgcc atcgccgcgg cggcaggtat cccccctgca gccatgcgcg
      781 caggcgttag tggatttact ggtgtgatgc accgcttgga gttcgtgcgc cgttggggtg
      841 gggcagattg gtataacgac tcgatcgcca cagcaccgga gcgcaccatc gcggctatcc
      901 gctcattcga ggagccgatc atactcctgg caggtggccg agataaggat ctgccttggg
      961 gtgactttgc tgcactggtc agccaccgtg tggatcatct ggtgcttttc ggggaagccg
     1021 cggagaaaat tggtctggcg atgggcaatt acccttatac acggtgtgcc aacctgaagg
     1081 aagcggtcca agcagccgtc catctggttg aaccagggga tgtcgtgctg ctttcaccag
     1141 gcggcactag cttcgatgag tttaaagatt tcgaggagcg tggagaatgt ttcaaacaat
     1201 tagtgatgtc tttataaaag gcggcaaagc caaccctgcg ggagcctcgt ctgtggaggc
     1261 acgtcgacgc actgta
//
LOCUS       DBNHOH010000124        10579 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_904260, whole genome shotgun sequence.
ACCESSION   DBNHOH010000124 DBNHOH010000000
VERSION     DBNHOH010000124.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10579)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10579)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10579
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_904260"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="AC16V7_02235"
     CDS             complement(<1..570)
                     /locus_tag="AC16V7_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012669.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR6255964.1"
                     /translation="MSKPLIPAQRRERIQEYLALHKIVASTDLSEWLGVSEATIRRDL
                     EWMEEEGFIERTHGGAILSQRLPLEPEYRQRAMRCPEEKRLIGLTAAAQIEEGDIVFI
                     NSGSTTTQIIRHIRRGSGITAITNNLYAALEFGETGLELLLLGGEYQHKSNSVAGHFA
                     IDNLSQVYANKAFIGVDGISPRFGYTVPTN"
     gene            850..1641
                     /locus_tag="AC16V7_02240"
     CDS             850..1641
                     /locus_tag="AC16V7_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255965.1"
                     /translation="MSGGQIGMGQQDREERERFENELQRILEASNEAGLTLRVIGSLA
                     FQIHCPSYGFLQQAMGRAYTDIDFAAYRKQAKDIKVLMAGLGYSEDREVFIVSEGDRA
                     IFNNPDNGLHVDVFFDKLDFCHLISWNERLEVDSPTIPLAEMILEKMQIVKINEKDVI
                     DTIMLLMEHPLGKVDEETINVDRIALLCAQDWGLWRTTTMNLDKVKQLAHTYSQLTDE
                     QKQQVVSQVEATLERIENEPKNLAWRLRARVGDRVKWYKDVDEVH"
     gene            1673..2497
                     /locus_tag="AC16V7_02245"
     CDS             1673..2497
                     /locus_tag="AC16V7_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR6255966.1"
                     /translation="MAKLVRDIMRPGVLTCSPDLTLGQVAVMLHKHHVHALFVTIAEP
                     GEQDSRILGVITDFDLLAGEWLSADRESLAVMRKMTAGELMSSPVDTIEADVPADEAA
                     RRMKEEVIRRLLVTDNGKPVGVISVSDFISSLAAAIPFGRKKVGDVMSDVYLCCRDKT
                     PLVSTARAMADTGWRSVLVVNAQGKPLGLFSGLDLLSLSAEGRADHTTVSEVMHPLLT
                     IAISASLRDAADMMIENHHHRLVVVDPKSPDSMPLGIISSFDIVAEMAHPESVWQK"
     gene            2580..5018
                     /locus_tag="AC16V7_02250"
     CDS             2580..5018
                     /locus_tag="AC16V7_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcvT family protein"
                     /protein_id="HIR6255967.1"
                     /translation="MTEIPSHAQVVIIGGGVGGCSIAYHLTQMGWKDVVLLERHELTA
                     GSTWHSAGLVGQLRSDANLTRMMSYSTDLYRRLKDETGQDTGWREVGGLRLASSPERM
                     EDLKRLVGMARSFGMPMELLSPQEALDLFPIMNIDGVLGAAFTPNDGMIDPTQLTNAL
                     AVGAKSRGARFYLDTDVEEIRLKNGRVDEVVTNRGSIKTEIVVNATGQWGGEVGKMVG
                     LNLPVVPMAHLYIITKPIEGVRHEFPTLRDPDLLVYWREEVGGLVTGGYERNPEPFGL
                     KGIPRDFKYQLLPPDWERFAPLMENSIKRVPVVETAEIIQLLNGPEGFTPDGEFLLGP
                     TSVKGFWVACAFCAHGLAGAGGIGKVMAEWIIDGQPEWDVWRLDVRRFGTNYASQDYT
                     VARTIETYSMYYDIHYPGEERLSRRNLRISPTYYRLRDLGAVFGEKTGWERPNYFKPY
                     EELAVHGHEPTGWARHNWSRAIGYEHLMTRQNAGLFDETSFNKFEVRGPGALAFLNYV
                     CANEIDVPVGSVVYTECLNKRGGIECDFTVTRLAEERFFIITGTAFGQHDMSWLRLHM
                     PEDGSVTIEDVSSSYTCIGMWGPKARAILQKTTSADVSNAAFPYMTAQSITVGDVPVL
                     ASRVTYVGELGWEFYCPIEYGLRLWDTLWEAGQPEGMVAAGYKAIDTLRLEKAYRYWS
                     GEISPDYNPYEAGLGFAVKLNKGDFLGREALLKQKESGLKRKLCCATLDDKRTIALGK
                     EPIRLLGTDNIVGWVASGGYGYSVEESIVFAYLPIEHSQPGTQLEIEFFGAQIVATVR
                     QMPLWDPKGERIRA"
     gene            5241..5969
                     /locus_tag="AC16V7_02255"
     CDS             5241..5969
                     /locus_tag="AC16V7_02255"
                     /inference="COORDINATES: protein motif:HMM:NF019322.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR6255968.1"
                     /translation="MFELTKIEGIPMYVWIREALRKEITGGVLKRGEKLPSEHDLASK
                     FGVSRMTVRHGIAELIDEGLLYRRHGVGTFVAFPHLERDHTRLTNFFDRTESEDIHAK
                     ATVLDLEVIPAKQKIAKALDITVGELVIRVKTLRFADNVPITIHDAHIPHKLFSKLID
                     ENLEVQNLWSLFEQSGYKVKRAIQRIEAKEATKELAKLLGIREGASVLYKERTVYADD
                     GTPVEFTYCYNRGDIYSLTVSLER"
     gene            6041..6967
                     /locus_tag="AC16V7_02260"
     CDS             6041..6967
                     /locus_tag="AC16V7_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR6255969.1"
                     /translation="MGLTIEQAIARVPFLAEANDIKITSLSGGITNLNYKIETDGKAY
                     VLRITGEGTDALGIRREVEYQANLAAGLLGVAPEVVYFIEPEGYLLTRYVKGKHIPPD
                     VITTPENIVRVVRKIRLFHRNAPQLDSEFNVFRRVEMLTATSKRNNCKFPFDFDWIMQ
                     KMRVAEGALLKDPYIPTPCHDDLLNLNWLDEDVPGEIGEIRLLDWEYAGMGDIFFDLA
                     NFSHHHRLNDDQVRLLLQEYFGEVTPKNFARLKIMWPMSEIHEAMWGTTQTGISKLDE
                     DFQGYADLWFGRARQHVTDPRWDKWLVDVAGK"
     gene            7084..7959
                     /locus_tag="AC16V7_02265"
     CDS             7084..7959
                     /locus_tag="AC16V7_02265"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0030388 - fructose 1,6-bisphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HIR6255970.1"
                     /translation="MSIVNAKEILVEAAKGKYAVGAFNITDLIQLEGVVDAAVEKKSP
                     LIIQTSVKPSKFLGTKVLVAIYKTIAESAPIPICLHLDHCTEVDYCKTCADAGYTNIM
                     IDASKQTFEENIRQTKEVVDYCHSVGDISVEGELGTVSGVEDQIKVAEDEAALANPVQ
                     GVEFVERTGVDIFAPAIGTAHGVYKTKNPKVDFERLGKINQMLNGGGVKTPLVVHGGT
                     GLPEDYIQKLIEMGGAKFNVSTELKRTLINSTYDYITAHREEYDPGKVDVAVRDAIRQ
                     AVGHWIDMLGSAGKA"
     gene            8053..9156
                     /locus_tag="AC16V7_02270"
     CDS             8053..9156
                     /locus_tag="AC16V7_02270"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR6255971.1"
                     /translation="MKSIEEYYVPWVKGIPMYISGHIELAWRQPELHRMMSNENPNPP
                     SEKVLATIDKYARLGHRYPDQGLVVRGKIAEINGLDGPDNVMLGNGSSEVFDNIFRVF
                     LAPGEEVIQHTPCFGIYKLRCNILGGKLVSVPMVYKDNQLLYDPDSILKAVTDKTKVI
                     VIANPNNPTGNFMDAKEFVRIAETGIPFVVDEAYIEYAGLGMSQVGLTKKYKNVIITR
                     TLSKAYGLAGLRFGYALGDKSVIGQISAALLPWNVGTIPMWAALAALEDTEALEYRVK
                     FNNSEVDFIQESLSDIPGLVIFRSQANYILFDAGGTGKRGKDMVAYAEQKGLIFRPDS
                     DKYGSDGWFRITIGSKEENRMAVAIVREFLTKS"
     gene            9265..10029
                     /locus_tag="AC16V7_02275"
     CDS             9265..10029
                     /locus_tag="AC16V7_02275"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HIR6255972.1"
                     /translation="MGKIKAVFFDQDGVIIDTERDGHRVSFNMTFKEFGFTDEWSVNY
                     YHELLQIAGGKERMKHHSLTKGFNPPVPPDEIDELVKTMHKRKTALFVELIESGQLPL
                     RPGVHRFMKEVVEAGLMIGVCTTSNEQSARAITGKILSDIKFDLVLAGDVVSKKKPDP
                     EIYNLALSRTGLKPDECFVVEDSKNGVLAAKAAGMPTVVTTNYYTEKEDVSSGDIIVT
                     SLGDPDGEKGVLTKGGDGLKFDGVLHVKQLVEYFEK"
     gene            10278..>10579
                     /locus_tag="AC16V7_02280"
     CDS             10278..>10579
                     /locus_tag="AC16V7_02280"
                     /inference="COORDINATES: protein motif:HMM:NF015953.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR6255973.1"
                     /translation="MNRFLIRWGINAVALYVAVALVPGIQPQSTNWLAFIWLALIFGV
                     VNAFLNPLLKFLTCPLILLTLGLFTLVINTGLFYLAGNIGSSFGVGFTVDNFWSAL"
BASE COUNT         2518 a         2537 c         3004 g         2520 t
ORIGIN      
        1 gttggttggc acggtatagc cgaaccttgg gctgatgcca tctacaccga taaaagcttt
       61 gttggcgtac acctggctca agttgtcgat ggcaaagtgc ccagccacag aattcgattt
      121 gtgctgatat tcgcctccca gcaggagcag ctctaagcca gtttcgccga actctaaggc
      181 ggcgtacagg ttgttggtga ttgccgtaat gcctgagcct cgacggatat gtcggatgat
      241 ttgtgtggtg gttgagccgc tattgatgaa gacaatatcc ccctcttcta tttgagcagc
      301 agcggttaag ccgatcaggc gtttttcttc cgggcaacgc atggccctct gcctgtattc
      361 cggctcaagc ggcaggcgct ggctcaagat ggcgccgccg tgtgtgcgtt cgatgaaacc
      421 ctcctcctcc atccactcca aatcacggcg aatcgttgcc tcggaaacac ccagccattc
      481 actcagatcg gtgctggcaa cgattttatg cagcgccaga tattcctgga tacgttcacg
      541 ccgctgggca gggatgaggg gtttgctcat gacgtgggaa tcgcaacctc tgcttagcgt
      601 ttgtgacgaa atttgattat attatcgaat aattatataa gattttgaag agatttgcaa
      661 gtttatatca tttcgtgtat aatgtcgctg agatgtctca gaagacccca aaggtttccc
      721 cgctggttat ccacgggtgg cgcatcatct cagcattacg tcgtagcagc tacgctggcg
      781 catgaaacct ttggggtctg ttccttttgt atcgagcggt tttctccgta tgttttagag
      841 gagtgtatca tgtcgggcgg tcagataggg atgggtcaac aggatcgaga agagcgggag
      901 cgctttgaaa atgagctcca gcgcattctc gaagccagca acgaagcagg tctgaccctg
      961 cgtgtcattg gatctttagc ttttcaaatt cactgtccca gttatggatt tctccaacag
     1021 gccatgggac gagcgtacac cgatatcgac tttgctgcct atcgcaagca agcaaaagat
     1081 atcaaggtgc tcatggctgg gctgggctac agcgaagacc gcgaagtctt cattgtcagc
     1141 gagggggatc gggctatttt caacaatcca gataatggtc tgcatgtgga tgtgttcttc
     1201 gacaagttgg acttctgcca cttgatcagc tggaatgagc gcctggaggt ggattcgccc
     1261 accattcctc tggcggagat gatcctggaa aagatgcaga tcgtgaagat caatgagaaa
     1321 gatgtcattg acaccattat gttgttgatg gagcacccgt tgggcaaggt ggacgaggag
     1381 acaatcaatg tggaccggat tgcactcctt tgcgcgcaag actggggatt atggcgcacc
     1441 accacaatga acttggataa agtaaaacag ttggcgcata cctattccca attgacagat
     1501 gagcaaaagc agcaagtggt ctcacaagta gaggccacct tggaacgcat cgaaaatgag
     1561 cctaagaatc tggcctggcg attgcgcgcc cgtgtcggtg accgcgtgaa atggtacaag
     1621 gacgttgacg aagtccatta agccataccg gtaaatattc aggaggaatg ctatggcaaa
     1681 gttggttcga gacatcatgc gtccgggcgt gttgacttgc tcgcctgatc tgacactggg
     1741 gcaggtggca gtcatgctgc ataaacatca cgtgcacgcc ttgtttgtca ctatcgctga
     1801 gccgggtgaa caggatagcc gtatcctggg tgtcattacg gattttgatc tattagccgg
     1861 ggagtggtta tctgcggatc gcgagagtct ggctgtgatg cgcaagatga ccgccggcga
     1921 gttaatgtct agcccggtgg acaccatcga agcggatgtc ccggcggatg aagcagcccg
     1981 ccgcatgaag gaagaggtta ttcgtcgctt gttggtaaca gataatggga aaccggtggg
     2041 ggtgatatcg gtttcggatt ttatctccag cctggctgcc gcaattcctt ttggtcgcaa
     2101 aaaggtaggc gatgtgatgt cagacgttta cctgtgctgc cgggataaga cgccattggt
     2161 ctccaccgca cgagctatgg cggatactgg ctggcgctcg gtgctggtgg tgaatgctca
     2221 gggaaagcct ttaggattgt ttagcgggtt ggacttgctc tcgctgagcg ctgaaggacg
     2281 tgccgatcat acaactgtgt ctgaggtgat gcatcccctg ctaaccatcg ccatttcggc
     2341 cagcttgcgt gatgccgccg atatgatgat cgagaaccac catcaccgtc tggttgttgt
     2401 cgatcccaaa tctcccgaca gcatgccttt gggcattatc tcttcatttg acatcgtagc
     2461 tgagatggct catcccgagt ctgtgtggca aaagtagatt agagggtgtc tcaaaaataa
     2521 gaggagggga taaggcttga tcttaaatta ttcaatcctg tcttcaagga gaatgcaaca
     2581 tgactgaaat cccttcgcat gcccaggtcg ttattattgg aggtggagta ggtgggtgca
     2641 gcattgccta tcatctcacg cagatgggtt ggaaagacgt agttcttttg gagcgccacg
     2701 agttgaccgc tggctccacc tggcattcgg ctgggttagt gggccagttg cgctccgatg
     2761 ccaacctgac ccgcatgatg tcttatagca cggacctgta tcggcggctg aaagacgaga
     2821 cgggacagga taccggctgg cgggaagttg gcggcctgcg cttagcctcc tcgcctgaac
     2881 gcatggaaga tctgaagcgg ctggtgggca tggcgcgttc gttcggcatg ccgatggagt
     2941 tgctcagccc gcaagaggcg ctcgatctct tcccaatcat gaatatcgac ggcgtgctgg
     3001 gcgctgcttt cactccaaat gacgggatga ttgaccccac ccagctgacc aatgctctgg
     3061 cggtaggggc aaaaagccgc ggagctcgct tttatctgga taccgacgtg gaagagattc
     3121 gcttgaagaa cggtcgtgtg gatgaggtcg tcaccaaccg cggcagcata aagaccgaga
     3181 tcgtagtcaa tgccacagga cagtggggcg gtgaggtggg taagatggtc ggcttgaacc
     3241 tgcccgtggt gcccatggcg cacctgtata tcatcacgaa gccgattgag ggcgtgcgcc
     3301 atgaatttcc cacgctgcgc gaccccgatt tgctggtgta ctggcgcgag gaggtgggtg
     3361 ggctcgtcac tggtggttac gagcgcaatc cagagccgtt tgggctaaaa ggcatcccgc
     3421 gcgattttaa ataccagttg ctgccgccag actgggagcg ttttgcgcca ttgatggaga
     3481 actctatcaa gcgtgttccc gtggtggaga cagctgagat cattcaattg ctcaatgggc
     3541 cagaggggtt cacccctgat ggtgagttct tgcttggacc gacttcggtc aagggtttct
     3601 gggtggcatg cgctttctgc gctcacggcc tggctggtgc gggtggcatc ggtaaggtga
     3661 tggctgagtg gattatcgat ggacaaccag aatgggatgt gtggcggctg gatgttcgcc
     3721 gctttggcac aaattatgcc agccaggatt acaccgtcgc gcggaccatt gagacctatt
     3781 ctatgtacta tgatatccac taccctggcg aagagcgcct atcccggcgc aacctgcgca
     3841 tctctcccac ttactatcgc ttgcgcgatc tgggagcagt ctttggagag aagactggct
     3901 gggagcgccc caattacttc aagccctacg aagagctggc tgtccacggg cacgagccaa
     3961 ctggctgggc acggcacaat tggtcacgcg ctatcggata tgaacacttg atgacgcgcc
     4021 aaaacgcggg cttatttgac gaaacatcat ttaataagtt cgaggtgcgc ggcccagggg
     4081 ctttagcctt cttgaactat gtctgcgcca acgagatcga tgtgccagtt ggcagtgtgg
     4141 tgtataccga gtgcctgaac aagcgcggtg gcatcgagtg cgatttcacc gtcacaaggc
     4201 tggcggaaga gcgtttcttt atcatcaccg gtactgcatt tggtcagcat gatatgtctt
     4261 ggctgcgcct gcatatgccc gaggatgggt cggtgacgat agaagatgta tcctcttcgt
     4321 acacatgtat cggaatgtgg ggacccaagg cgcgggcgat cttacaaaaa accaccagtg
     4381 cagatgtctc taacgctgct tttccctata tgacggcaca atccatcacg gtgggcgacg
     4441 tgcccgtact ggcttcacga gtcacatacg tgggcgagct aggctgggag ttttactgcc
     4501 cgatagagta cgggctgcgc ctgtgggata cattgtggga agccggccag ccagaaggca
     4561 tggtggcggc aggttacaag gctattgaca cactccgctt agagaaagcc taccgctact
     4621 ggagcggtga gatcagcccg gattacaacc cctatgaggc tggattaggc ttcgcggtga
     4681 agctgaacaa gggtgacttc ttgggtcgcg aggcgctgct aaaacagaag gaaagcgggt
     4741 tgaagcgcaa gctgtgctgt gcaacgctgg atgataagcg cactatcgca cttggcaagg
     4801 aacctatccg cctgttaggt acagataaca tcgtcggttg ggtcgcctcg ggcggctacg
     4861 gctattcggt tgaggagagc atcgtctttg cctatttgcc tatagagcac agccagcctg
     4921 gcacacagtt ggagatcgag tttttcggtg cccagattgt tgccaccgtc agacagatgc
     4981 cgctgtggga tccgaaaggc gagcgaatcc gggcgtagtc attaaagcag ctcatatcct
     5041 agccctttct tcactggtag aggggagatc gagctgaaaa atcgtgtctg cgaatgcgca
     5101 atcatgattt tcggcgaatc ttccctctat agagatttat gggtagggag gaaatcggct
     5161 gcgtccattg acaaatataa atctccattt gtataatata gatgtctata cgtctagata
     5221 tctgacaaat gagaacgtaa atgtttgaat tgaccaaaat tgagggcatc cctatgtatg
     5281 tatggataag ggaggccctg cgcaaggaga tcaccggggg tgttttgaag cggggcgaga
     5341 aactaccctc tgaacacgat ttagcttcaa aatttggtgt cagccgcatg acggtgcgtc
     5401 acggcatcgc agagctgatt gacgaaggct tgttgtaccg gcggcacggt gtgggaactt
     5461 ttgtcgcctt tccccacctg gaacgtgacc atacacgcct gaccaatttt tttgatcgta
     5521 ccgaaagcga agatattcat gccaaggcga ccgtgctgga tttagaggtg atccccgcga
     5581 aacaaaagat tgctaaggct ctggatatta cggttggcga gcttgtgatc cgtgttaaga
     5641 ccctgcgatt tgccgacaat gtgcccatca ctatccacga tgcgcatatc ccgcacaaat
     5701 tgttctcaaa actgattgat gaaaatctgg aggtgcaaaa tctctggtcg ctatttgagc
     5761 agagcgggta caaagtcaaa cgtgccatcc agcggatcga ggccaaggaa gccacaaaag
     5821 agctggcaaa attattggga attagggaag gtgcgtctgt tctttataag gagcgcaccg
     5881 tttatgccga tgatggcacg cctgtggagt ttacctactg ctacaaccgc ggcgatatct
     5941 attcgttgac ggtgtcactt gagcgttgac agttaatgtg gtaggttggt catggtagag
     6001 tatttatcga tcggcatgat cttattgatg gaggatttaa atgggtttga caattgagca
     6061 agctatcgcc agggtgccat tcctggcgga ggcaaatgat ataaaaatca catcgctatc
     6121 tggtggaatt accaacctaa actataaaat cgagacagac ggtaaagctt acgtcttgcg
     6181 tatcactgga gaaggtacgg atgcgttggg tatccgccgc gaggtcgaat accaagccaa
     6241 cttggctgcc ggtctattgg gtgttgctcc tgaggttgtc tattttattg agcctgaagg
     6301 ctacctgctc actcgctatg ttaaagggaa gcacatccct ccggatgtga tcacaacgcc
     6361 tgaaaacatt gtgcgcgtgg tacgcaagat ccgattgttc cacaggaacg cgccacaact
     6421 ggacagcgag tttaacgtat tccgccgcgt ggagatgctc actgccacct ccaagcgcaa
     6481 caactgcaag ttcccattcg acttcgattg gatcatgcag aagatgcgcg tagcggaagg
     6541 tgcattgctc aaagatccct acatccccac cccctgccac gatgatctgc tcaacctgaa
     6601 ctggctggat gaagatgtgc ccggtgagat cggcgagatc cgcttgctcg actgggaata
     6661 tgccggcatg ggtgacatct tcttcgactt ggcgaatttc agccaccatc accgcctgaa
     6721 cgatgatcag gtaaggttgc tcttgcagga gtattttggc gaggtgacgc cgaagaattt
     6781 tgctcgcctt aagatcatgt ggcccatgtc ggagattcac gaagccatgt ggggcactac
     6841 ccagacggga atctccaaac tggatgagga cttccaaggc tacgccgatc tgtggttcgg
     6901 gcgcgcgcgc cagcatgtca cagatcctcg ctgggataag tggctggtgg atgtagctgg
     6961 aaaatagatg gcattacgct gggtccgctc ttcattggga aagaccccag cgctgaacgc
     7021 aaatacgccc acaacgcatt tgtgggcaaa agaaaagata cgttgcagta taggaggccg
     7081 tatatgtcaa tcgtaaacgc aaaagaaatc cttgtggagg cagcgaaggg caaatacgcc
     7141 gttggcgcct tcaacatcac cgacctgatc cagttggagg gagtggtgga cgcggcagtt
     7201 gagaagaagt ccccattgat catccagacc tcggtgaaac catccaaatt cttgggcacg
     7261 aaggtgctgg tggcgatcta caagacgatc gctgagtcag cacccattcc gatctgcctg
     7321 catctggacc actgcacaga agttgactac tgcaaaacgt gcgctgatgc gggttacacg
     7381 aacatcatga tcgatgcatc taagcagacc tttgaggaga acatccgcca aaccaaggag
     7441 gtggtggatt actgccactc ggtcggtgat atctctgtgg aaggcgagct gggtacggta
     7501 agcggcgtgg aagatcaaat caaggtcgct gaagacgagg ctgccctggc aaatccagtc
     7561 cagggggttg aatttgtcga gcgcacgggc gtggatatct ttgcccctgc cattggcacg
     7621 gcccacggcg tgtataagac caagaatccc aaggtggatt ttgagcgcct gggcaagatc
     7681 aaccagatgc ttaacggcgg tggagttaag acaccgctgg tagtgcatgg gggtaccggt
     7741 cttccggaag attacatcca gaaattaatt gaaatgggcg gcgccaagtt caacgtttcc
     7801 acggagctca agcgcaccct gatcaattct acctatgact atatcacggc acatagggaa
     7861 gaatatgatc ccggcaaggt cgatgtagct gtccgcgacg ctatccgcca ggctgttggg
     7921 cattggatcg atatgctcgg cagcgcaggg aaagcataaa aaacaatgaa agcttcgtag
     7981 tgaggagcca ttgaacctcc tcactacgca ttctttacta tacttgcaac ctaattatca
     8041 aggagttcca acatgaaaag tattgaagag tattatgttc cttgggtgaa gggtatcccg
     8101 atgtacatat cagggcacat tgagcttgcc tggcgccagc cggagctgca tcgcatgatg
     8161 tctaacgaga acccgaaccc gccatccgag aaagtgctgg cgaccattga taagtatgcc
     8221 cgcctgggtc atcgctaccc tgaccagggt ttggtggtgc gcggtaagat cgctgagatc
     8281 aatggcttgg atgggccgga taacgtgatg ctaggcaacg gctccagcga agtgtttgat
     8341 aacatcttcc gcgtctttct ggctcctggc gaagaggtga tccaacacac accctgcttt
     8401 ggcatctata aactgcgctg caacattctg ggcggcaagc tggtttctgt tcccatggta
     8461 tataaggata accagctgct ctacgatccc gattccatcc tcaaagctgt cactgataag
     8521 accaaagtca ttgtcatcgc caatccgaac aaccctacgg gcaacttcat ggatgccaag
     8581 gaatttgtgc ggatcgctga gacgggtatc ccatttgtcg ttgacgaggc ctacatcgaa
     8641 tatgccgggt tgggcatgtc tcaggtagga ctgaccaaga agtataaaaa cgtgattatt
     8701 acgcgcacgc tgtccaaagc ctatggcctg gcgggattgc gctttggcta tgccctggga
     8761 gataagagcg ttattgggca aatctccgca gcgctgcttc catggaatgt gggcaccatt
     8821 ccgatgtggg ccgcgctagc agccttggaa gataccgaag ccttggagta ccgggtgaaa
     8881 ttcaataaca gcgaggtgga ttttatccag gaatcgctga gcgatatccc tggattagtc
     8941 attttccgct cacaagccaa ctatatcctc ttcgacgctg gggggaccgg caagcggggc
     9001 aaggatatgg tggcgtacgc cgaacaaaaa ggtctgatct tccgcccaga tagcgataaa
     9061 tacggaagcg atggttggtt ccgcattacc attggcagta aggaagagaa ccgcatggcg
     9121 gtcgcgatcg tgcgcgaatt cttaaccaag tcctgagctt ttcgcaatcc taagctcaga
     9181 agagggtccg ctaaatacgc taattttcat aaaaagcttg actttcatta gcgaaaatga
     9241 gtggatcaaa cctggaggcg aatcatggga aaaattaaag ctgtgttttt tgaccaggac
     9301 ggtgtcatca tcgacaccga gcgggatggt catcgcgtct ctttcaatat gacctttaaa
     9361 gagttcggct tcaccgacga atggagcgtg aattattatc acgagctgct gcaaatcgcc
     9421 ggtggcaaag aacgcatgaa gcatcacagc ctgacaaagg gcttcaaccc accggtgccc
     9481 cccgacgaga tcgatgagct ggtaaagacc atgcacaagc gcaagacagc gttatttgtc
     9541 gagctcattg aatcgggaca actccccctg cgaccgggcg tgcaccgctt tatgaaggaa
     9601 gtggtggagg cagggctgat gattggcgtg tgcacaactt ccaacgagca gtccgctagg
     9661 gctatcacgg gaaaaatcct ctccgacatc aagttcgatc tggtgctggc aggcgatgtg
     9721 gtgagcaaga aaaagcccga tccagagatc tataacctgg cactctccag gaccggactc
     9781 aagccggatg agtgcttcgt ggttgaagat tcgaagaacg gtgtgctggc tgcgaaggcg
     9841 gcggggatgc caactgtggt aaccacgaac tactacacag agaaagagga tgtgagctcc
     9901 ggagatatca tcgtcaccag cctgggcgac cccgatgggg agaaaggcgt gctcaccaag
     9961 ggtggcgatg ggctaaaatt tgacggtgtg ctgcacgtca agcaactggt ggagtacttc
    10021 gagaagtaaa tcagggtaag ctacccttgc gatggtgctc cttttgggtt atggtagggt
    10081 aagttgccaa cttgccctac catttcttta agccaaagtg cgcattatcg tggataagcg
    10141 catccttcgc gactaagcaa accaaatttc gtaaatagtt atgcagtgct cctgcaaacc
    10201 gagatcctga ggatcgctca gatcctcagg gtctcggcaa gatggttgtc tctttagtga
    10261 aaagaaagga ttataagatg aaccgttttc tcatccgctg ggggatcaat gccgttgctt
    10321 tgtatgtggc tgtcgcattg gtgcctggaa tccaacccca gagtaccaac tggctggctt
    10381 tcatctggct ggcgctgatc tttggtgtgg tcaatgcctt tctaaacccg ctgctcaagt
    10441 ttttgacctg tccgttgatc ctgcttacac tgggattatt cactctggtc atcaacacgg
    10501 gtttattcta tttggctggt aacattggct catcttttgg tgtgggtttc acggttgaca
    10561 acttctggtc tgccttgtt
//
LOCUS       DBNHOH010000125         8081 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_840080, whole genome shotgun sequence.
ACCESSION   DBNHOH010000125 DBNHOH010000000
VERSION     DBNHOH010000125.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8081)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8081)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8081
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_840080"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1249)
                     /locus_tag="AC16V7_02285"
     CDS             complement(<1..1249)
                     /locus_tag="AC16V7_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR6255974.1"
                     /translation="MFGGQDGLRRVMSRETIKPRKVGETLARLGGYFKPYWYALVLTA
                     ILAVASTWAQVVTPELTGQVVDCFLTPAAESAFTSFSGLPGTPVSAGQNGAAAANCWL
                     AAGKPSAGLSQTIVKSLLSVVSFQAPNLEPGQMNTAERLIGMGQLILLITIIFIFGSI
                     LVGLTFFTMSWAGQHVLRSLRVDVFRHLHRLPLSYYAEQEAGDLMSRITNDSEAIQQA
                     LSFALVNVATGALLLGWIAYNMLARSLVFGLLSLIVVPIMSIATFWFSDQARKAFRRT
                     RQEMGSVNAELQESIAAVREVQAFNRAEENIENFRITNAANRDANIRAVAFTSALAPT
                     LEALGYLALAIVTGVGGLALLRDEPLFGTAVSLGLVITFLGYVQRFNQPIQQIAVLWT
                     NLQNAIAGGERIFNLLDVEPAIQD"
     gene            complement(1345..3297)
                     /locus_tag="AC16V7_02290"
     CDS             complement(1345..3297)
                     /locus_tag="AC16V7_02290"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6255975.1"
                     /translation="MRNRRISNSQRTIQRQALGRAVRYLARYRRQAVLPYLFLLIAAL
                     AQLAVPRLVRNIIDAVTRGVMAKSMLAGLNQIPAALLPQALPTILQTFGLPNDWTAEQ
                     LLSKLAADIATAPRLMVSAAVAIVIFAALRGLFAFLQVYLAERNSQSVAFDLRNDLFA
                     KIQRLSFSYHDRNQTGQLMIRATDDVEKVRIFIGQGLLQLAGALVLLAGTVILLLVTN
                     LRLALVILPILPVALVLFILFGSLSQPLFAKVQIKLSTLNTVLQENLAGIKVVKAFTR
                     ERSEQVKFQTAADALMHQQIGISRLFTFLFPLVFLTANLGQASILYFGGRQIIAGTLS
                     LGEWQEFSLYLIYLFLPIAQLGIIITQFGQASASAARIFEILDAHSDVSDLPGAIQLP
                     PVQGSIRFENVTFRYFSSGEPVLKNVSFEALPGQTVALLGATGSGKTTIINLLPRFYD
                     PSEGRISIDDHDLRQVTLDSLRSQIGIVLQETTLFAGTIRDNIAFGKPEASQEEIIAA
                     AKAAAAHDFIMSFPQGYDTPVGERGTTLSGGQKQRIAIARALLLDPHILILDDSTSSV
                     DLATEAQIQAALDDLMKGRTSFVIAQRISTVMNADLILVLDKGAIVARGQHAELMEES
                     PIYAEIYNSQLIGDVEVSVSPLASEA"
     gene            complement(3417..3872)
                     /locus_tag="AC16V7_02295"
     CDS             complement(3417..3872)
                     /locus_tag="AC16V7_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR6255976.1"
                     /translation="MPSTEQFTQVLRDWAEVFMRRSMSDFKRFIDDSGLSPSQISALM
                     RLYYGGKCGVSDIGNHLGVTNAAASQMVDRMVQLGLFTRNEDTGDRRVKQIALTSQGR
                     ELVEKGVESRRRWMEKLTTTLSPEEQDAIIAALMVLTGAARHLESEDIT"
     gene            complement(4013..6970)
                     /locus_tag="AC16V7_02300"
     CDS             complement(4013..6970)
                     /locus_tag="AC16V7_02300"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6255977.1"
                     /translation="MTKLALYLLGAPLLENDGQLVAFDTRKAMALLAYLAVTTKVHSR
                     DSLAALFWADYAQSSARAALRRTLSVLNKALREDTLEITRDRLGLAQGAALWFDVEEF
                     QRLLKERETHGHPQREICSRCLDCLEKAVDLYRGDFMAGFTLRDSPEFDDWQYFQTDS
                     LRRELAGALEQLAHGLAAKAEYERAIVHARHRVALDPLHEAAHRQLMQLYAWAGQQSA
                     ALRQYQECTRILDKELGVPPMPETTQLYQAIKEQRAQEPLTTEYQFAERPQSLESIAP
                     PPSRAAAHMLPLVGRWQEMASIKDNYAGIQSDGYFQILEGEAGIGKTRLAEEFLSEAG
                     TRGAFSIMARCYSGESNLAYAPFLEGLRAALAHAHPNWHQPIQAHWLVEAARLLPELS
                     RLRPDLPAAPSLDSPGAQTRFFEGVSQVLQALCDSQPPGILFFDDLQWADEASLDLLT
                     YLVRRLHGRRLFVLAVWRGEDLPMEHRLRRLLAEAQRNGYASAITLSRLDTKSIMELV
                     KFVAGEAGLPLENMTLRLFEETEGLPFFITEYLATLPEVDTGLQDDWTMPQGVRDILH
                     SRIDALDDAGRQLLQTAAVIGRSFDFETLQYASGRSEEETTTTLEALIASGLIRETQP
                     GRERRASDLLRTLDYDFSHDKTRALVYEETSQGRRRLLHQRVADGLLRHSRSRQGMRL
                     VAGQLAYHYLQSGRAKEAAEYSKLAGEHARSVYANTEALAHYQAAIALGHPDIAELEE
                     AMGDLYTLLGNYEAALGSYQTAAGRSTASSQGISRMRHKMGDIYHRRGEWELAEDSFQ
                     AAAQDMKAQDDAARLSRLYADWSRTMRRRGQLERALHTASQSLDLAEKVDDDQALAQA
                     HNILGMLLRSQGNLDEAIMHLERSLLIAEKLPEPGIRIAALNNLSLAYADHGELRRAL
                     AYTQQALELCAIQGDRHRAAALHSNLADLYHSAGQEEQSMAHLKQAVVIFSEIGASET
                     ERPRPEIWKLTEW"
     gene            7294..>8081
                     /locus_tag="AC16V7_02305"
     CDS             7294..>8081
                     /locus_tag="AC16V7_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6255978.1"
                     /translation="MKKVLKWIGIALGGLIGLLVIAVIGLALYASSKFKPTYVNRPLY
                     QITADTSPEGVARGKYLMEEAMLCAEACHSPEGKPFQGYIEEISEGPIQVLFAVPNLT
                     PDVETGLGSWSDAQIARAIREGVDKDGVGLVVMPAYNYKALSDADVAAIIGYLRNLEP
                     VHHEIPPISANIVAKVMMAMGAFGPSSVGEPITAPKDTPPAGTPEYGQYMLALGACSD
                     CHKQNLAGGPIPFAEEGMPWSANLTPAGELANWSEADFIAAVSEG"
BASE COUNT         1695 a         2211 c         2373 g         1802 t
ORIGIN      
        1 agtcctggat ggcgggctcc acgtccagga ggttgaaaat gcgctcccca ccagcaatag
       61 cattctgtag gtttgtccac agcacggcaa tctgctggat aggctggttg aagcgttgca
      121 cgtaccccag aaaggtgatc accaaaccca acgagactgc cgtgccgaag agcggctcgt
      181 cgcgtaacaa agccagcccg ccaacgccgg tgacgatagc cagcgccagg tagcccagtg
      241 cctctagggt gggcgccaaa gcgctggtga aggccaccgc gcggatatta gcatcccggt
      301 tggcggcatt ggtaatcctg aagttctcaa tattttcctc agcacggttg aaagcctgca
      361 cctctcgcac tgctgcgatg ctctcctgca gctcggcgtt tacgctgccc atctcctggc
      421 gggtgcggcg gaaggctttg cgcgcttggt ctgagaacca gaacgtggcg atggacatga
      481 ttggcacgac gatcaggctg agcaggccga aaacgaggct gcgcgccagc atattgtatg
      541 cgatccagcc cagcagcagc gccccggttg ccacgttgac gagggcgaag ctgagtgcct
      601 gttggatggc ctcggagtca ttggttatgc ggctcatcag atcgccagct tcctgctcgg
      661 cgtaatagct cagtgggagg cggtgcaggt gacggaagac atccacacgc agggagcgca
      721 atacgtgttg gccagcccaa ctcatggtga aaaaggtcag gccgaccagg attgatccaa
      781 agatgaagat aatcgtaatg agcaagatca attgccccat cccaatcagg cgctcagcgg
      841 tgttcatctg cccaggttcc aaattgggag cctgaaagct gactacagac aataagcttt
      901 taacgatggt ctggctgagg cctgccgatg gtttacctgc tgccagccag cagttggcag
      961 ccgccgcgcc gttctgccca gccgagacgg gcgttcctgg cagccccgag aagctggtaa
     1021 aggcgctttc cgctgcgggt gtcagaaagc aatctaccac ctgcccagtt aactctggtg
     1081 tgaccacctg tgcccaggtg gaagccactg ccagtatcgc cgtaagcacc aatgcgtacc
     1141 aataaggctt aaaatagccg cccaggcgag ccagggtctc gcccactttg cgaggcttga
     1201 tcgtctcccg gctcatcacg cggcgcaatc catcttgtcc accgaacatg ttttgctccc
     1261 aaataccttc ttattgaatt ccttgtccta cattgtgcgg cgtgtaatgt tcgcgcacga
     1321 tgctgcttgt ctttcgaagt gcggtcacgc ttcactcgcc agcggtgaca ctgaaacttc
     1381 cacatcccca atgagttgag agttgtatat ctcggcataa atggggcttt cttccatcaa
     1441 ctcggcatgc tgacctcggg caacgatagc gcccttatcc agaaccagga tcaggtcagc
     1501 attcatcacg gtgctgatgc gctgggcaat gacgaagctg gtgcgccctt tcatcaggtc
     1561 gtccagggca gcctggattt gcgcctcggt tgccaggtcg acgctgctgg tcgagtcgtc
     1621 caggatgaga atatgtggat ccaacagcag ggctctggcg atggcaatgc gctgtttctg
     1681 cccgccgctt agcgtggtgc cgcgctctcc caccggggtg tcatagcctt gcgggaagga
     1741 catgataaag tcgtgagctg ctgcggcttt ggcggcggcg attatctctt cttggctggc
     1801 ttccggcttg ccgaatgcga tgttatcccg gatggtgccg gcgaaaaggg tggtctcttg
     1861 caggacaatt ccgatctgcg agcgcagcga atccaaggtc acctggcgca ggtcatgatc
     1921 gtcaatggaa atgcgcccct cgctgggatc gtagaagcgc ggcagcaggt tgatgatggt
     1981 ggttttacca gagccagttg cgcccagcag ggcgacggtc tgcccaggca acgcttcgaa
     2041 gctcacgttt ttcagcactg gttctccgct actgaaatat cggaaagtga cattctcgaa
     2101 gcggatgctc ccttgtacag gcggcagttg gatggcacca ggcagatcgc tcacgtcgct
     2161 gtgtgcatcc aggatttcga agatgcgtgc ggcggaggcg gaagcttgac caaattgcgt
     2221 gatgatgatg ccgagctggg caataggtaa aaacaggtaa attaagtaca ggctgaactc
     2281 ctgccactcg cccagcgata gcgtgccggc aataatctgc ctgccaccaa aatacaggat
     2341 ggaggcctga cccaggttgg cggtcaagaa caccagtggg aaaagaaaag taaataacct
     2401 ggagataccg atctgctggt gcatcaaggc atcggcagcc gtctggaatt tcacctgctc
     2461 gcttcgctct cgagtgaaag cctttaccac cttgatgccg gccaggttct cttgcagcac
     2521 ggtgttcagc gtcgagagct ttatctgcac cttggcgaat aaaggctggc tgagggagcc
     2581 gaagaggatg aataagacca gggcgaccgg caggataggc aggatgacca gcgccaggcg
     2641 caggttggtc actaaaagca gtatcacagt gcccgccagc aacaccagcg cacccgccag
     2701 ttgcagcaag ccctggccga tgaaaatacg aaccttttcc acatcgtctg tggcgcggat
     2761 catcaactgc ccggtctggt tgcggtcatg gtaggagaat gagaggcgct ggatcttggc
     2821 aaacagatcg ttgcgcaggt cgaaggccac gctctgcgag ttccgttctg ccaggtagac
     2881 ctgcagaaaa gcgaacaggc ctcgcagggc ggcaaagatc actatcgcga ctgccgcgct
     2941 gaccatcaag cggggtgcag tggcaatgtc ggctgccagt ttggacagca attgctctgc
     3001 ggtccaatcg tttggcaggc cgaaggtttg caagatggtg ggcaaagctt gaggtaaaag
     3061 ggcggcgggg atctgattta gcccggcgag catggatttt gccatcacac cccgcgtcac
     3121 cgcatcgatg atgttgcgca ccaggcgcgg caccgccagt tgtgccagcg cggcgatgag
     3181 caggaagagg tagggcaaca ctgcctgcct tcggtagcgt gccaagtaac gcaccgcccg
     3241 acccaacgct tggcgctgga tagtgcgttg cgagttgcta atgcgtctgt ttcgcaaaaa
     3301 gttctccttt tcccgtcatt ctctaccgaa ataacgagtt gttagctggc tcgattttcg
     3361 gatgactctc ttttttgcct gtatcttatg gaagagaaag gggctatggg atgggcttaa
     3421 gtgatatctt cagattccaa gtggcgggct gctcccgtta gcaccatcag cgcagcgatg
     3481 atcgcatcct gctcctcagg gcttagggtg gtggtaagct tctccatcca acggcgtcgg
     3541 gattctacac ccttctctac cagttcccgc ccttgagagg tgagagcaat ttgctttacc
     3601 cgccggtcac cagtgtcttc gttgcgcgtg aacaagccaa gttgcaccat gcggtctacc
     3661 atctgactgg cagcggcatt ggttactccc aggtggttgc cgatgtctga aacaccgcat
     3721 ttccccccgt aatataagcg cattaaggcg ctgatctggg aaggggacag gccggaatca
     3781 tcgataaagc gcttgaagtc gctcattgac cggcgcataa acacttcagc ccagtcgcgt
     3841 aatacttgtg taaattgctc tgttgaaggc atattggtta atccatctaa ttagttaaac
     3901 gggcttaact atatactgct gggttaattt tgtcaatata gcaggggttt acgcagttga
     3961 tggagaggaa gggggataag gggggcgaca aaacgcgagc tattgtcccc ttttaccact
     4021 cggtcagctt ccagatctct ggtctggggc gctcggtctc actggctccg atctcgctga
     4081 agatgaccac agcttgcttg aggtgcgcca tcgattgttc ctcctgcccg gcagagtgat
     4141 acaggtcagc caggttgcta tgcagggcag ctgcgcggtg gcggtctccc tgaatggcgc
     4201 acagctccag tgcttgctgt gtgtatgcca gggcgcgccg cagttcacca tggtcggcat
     4261 acgccaggga gaggttattg agtgccgcga tgcggatacc tggttcgggt agtttttctg
     4321 ctatcagcaa gctccgctcc aggtgcatga ttgcctcatc cagattcccc tggctgcgca
     4381 gcaacatgcc taagatattg tgcgcctgtg ccagggcctg gtcgtcgtct accttttcgg
     4441 ccagatccag agactgggat gccgtgtgta gcgcccgctc tagttgcccg cgccgtcgca
     4501 tggttcgact ccaatcggca tacaggcgag aaaggcgtgc tgcatcgtcc tgggctttca
     4561 tgtcttgggc agcggcttga aagctgtctt cggctaattc ccattcaccc cgccggtgat
     4621 atatatcccc catcttgtgc cgcatgcgag aaataccctg gctgctggct gttgagcgtc
     4681 ccgcggcggt ttgatagctg cccagtgcgg cctcataatt tcctaatagg gtatacagat
     4741 cgcccatggc ttcttccagt tccgcaatgt cggggtgacc taaggcaata gcggcttggt
     4801 agtgggccag ggcttcggtg ttggcataga ccgagcgagc atgctcgccg gccagcttgc
     4861 tgtactctgc agcttctttg gcgcgcccgc tttgcaggta gtgataagcc agctgcccgg
     4921 caaccaggcg cattccctgc cggctgcggg aatggcgtag caggccatcg gccacccgct
     4981 ggtgtagcag gcgccgccgc ccctggctgg tttcttcata gacgagcgcg cgcgttttat
     5041 cgtggctgaa gtcataatcc agggtgcgta gcaggtcact ggcacggcgc tcgcgccctg
     5101 gctgggtttc tcggatcaag ccactcgcga tcagcgcttc cagggtcgta gtggtttcct
     5161 cctcgcttcg cccgctggca tactgcaggg tttcaaaatc gaaggaacgc ccgatcaccg
     5221 ccgcggtctg gagaagctgc cgcccggcat catcgagagc atctatgcgc gagtgaagga
     5281 tatcgcgcac gccctgcggc atagtccagt cgtcctgcaa cccggtatcg acctccggca
     5341 aggtggccag gtattctgtg atgaagaagg gtaaaccttc ggtctcttcg aagaggcgta
     5401 aagtcatgtt ctccagtggg agacccgctt ctccagctac gaacttcact aattccatga
     5461 tcgattttgt atccaggcga gatagcgtga ttgcgctggc gtagccattg cgttgcgcct
     5521 cggctagcaa cctgcgcagc cgatgttcca ttggtaggtc ttcaccgcgc cacacagcca
     5581 gcacaaacag acgccgtccg tgcaggcggc gcaccaggta tgtcagcagg tcaagcgagg
     5641 cctcatccgc ccattgcaag tcgtcgaaga acaagattcc tggtggctgg ctgtcgcaaa
     5701 gtgcctgcaa aacctggctc acgccttcaa aaaagcgcgt ttgtgcccct gggctatcta
     5761 atgagggagc ggcaggtaaa tcggggcgca gtcggctaag ctctggcaag aggcgcgcgg
     5821 cctcgacgag ccagtgcgcc tggattggtt ggtgccagtt tggatgggca tgggctaagg
     5881 ctgctcgtaa tccttcaagg aaaggtgcat aggccagatt ggattcgcca gaatagcagc
     5941 gcgccatgat agagaaagcc ccgcgcgtgc ccgcttcgga caggaattcc tccgccaggc
     6001 gggttttgcc gatacccgct tcgccctcca gaatctggaa atagccatcg gactgtatcc
     6061 cggcgtaatt atcctttata gatgccatct cttgccagcg tccaaccagg ggaagcatgt
     6121 gggctgctgc ccgggaaggt ggaggggcaa tgctttcaag ggattggggg cgctctgcaa
     6181 actgatattc cgttgtgagg ggttcctgag cgcgttgctc tttgatggct tggtagagtt
     6241 gggtggtctc tggcataggt ggcaccccca actccttatc cagaatgcgg gtgcactctt
     6301 ggtattgtcg tagggcagca gattgctgtc cagcccaggc gtaaagctgc atcaattggc
     6361 ggtgggctgc ttcatgtagc gggtcgagcg ccacgcgatg gcgtgcatgc acaatggcac
     6421 gctcatactc cgcctttgca gccagaccgt gagccagttg ctctagggcg ccagccagtt
     6481 cccgtcgcag ggaatcagtt tggaagtact gccagtcgtc gaattcgggg ctgtcgcgca
     6541 gggtgaagcc agccatgaaa tcgccccgat aaagatctac ggccttctct agacagtcca
     6601 agcagcgcga gcagatctct ctttgagggt gaccgtgggt ttcgcgttcc ttgagcaagc
     6661 gctgaaactc ttccacgtcg aaccataacg ctgcaccttg cgccaagccc aacctgtcgc
     6721 gggtgatctc cagggtgtct tcgcgcaggg ccttgttgag gacagacagc gttcggcgca
     6781 aggcggcgcg cgcgctggac tgggcgtaat ctgcccagaa cagcgcggcc agcgaatcgc
     6841 ggctatgaac cttggtggtt acagccaggt aagccagcag ggccatcgcc ttgcgcgtat
     6901 caaacgcgac aagttgacca tcattctcta ggagcggagc tcccagcagg tagagagcca
     6961 gtttcgtcaa tctgtttcag cccttcatgt tctcgtgccc acccagtgat ggggtattga
     7021 aaccagaata ttgtagttga tattctggga caaatggcct tattccgctt agattttact
     7081 gcataaatat agcctcccgt aacgcttccc gtaacgagtt agggacgaaa ggatgttaaa
     7141 ataagccata caagatgaaa aatatccatt tatctatgag tagaggtgaa attcacatca
     7201 tcctgttgga aactgattga tcaatagctt tccagcggtt acggtgacag gtgtgtctcc
     7261 gccgagtatc tcatttttta ggagaaaata aagatgaaaa aagtattgaa atggattggt
     7321 attgctttgg gcggtctgat cggcctgctg gtgatcgcag tcatcggcct ggcactatat
     7381 gcttcgtcga agttcaagcc cacctatgtc aatcgtcctc tctatcagat cacagccgat
     7441 accagcccag agggtgtggc gcggggtaaa tatctgatgg aagaagccat gctgtgcgcc
     7501 gaggcttgcc actcccctga aggcaagcct tttcaaggat acatcgagga gatcagcgag
     7561 ggaccgattc aggtcctatt tgccgtgccc aacctgaccc ctgatgtgga aaccggtctt
     7621 gggagttgga gtgacgccca gattgcccgc gccatccgcg aaggtgtgga caaagacggt
     7681 gttgggctgg tggtgatgcc agcttacaac tacaaggctt tgagcgatgc tgacgtggcg
     7741 gcgattatcg gttacttgcg caatttggag ccggtgcacc acgagatccc acctatctcc
     7801 gccaacatcg tcgccaaggt gatgatggcg atgggcgctt ttggccccag ttcagttggc
     7861 gagccgatta cagccccaaa ggatacccct ccggctggca cgcctgaata cggccaatat
     7921 atgcttgcct tgggcgcctg ctcagactgc cacaaacaga atttggcagg tggccctatt
     7981 ccctttgccg aagaaggtat gccatggtct gctaatctga ccccagcggg cgagcttgcc
     8041 aattggagcg aggctgattt tattgccgct gtgagtgaag g
//
LOCUS       DBNHOH010000126         1555 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1417520, whole genome shotgun sequence.
ACCESSION   DBNHOH010000126 DBNHOH010000000
VERSION     DBNHOH010000126.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1417520"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            324..536
                     /locus_tag="AC16V7_02310"
     CDS             324..536
                     /locus_tag="AC16V7_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255979.1"
                     /translation="MNPKEAIAFDADELLELEMILVDGDAQAALEFLKRAVWKKIQLA
                     RKGRLKCHLDYGTTDTVADFEKKERT"
     gene            533..892
                     /locus_tag="AC16V7_02315"
     CDS             533..892
                     /locus_tag="AC16V7_02315"
                     /inference="COORDINATES: protein motif:HMM:NF016326.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HIR6255980.1"
                     /translation="MNAPVVPKIDNYRFGNIVIDGKQFTSDVIIYPDRVEGSWWREEG
                     HSLALSDLPAVLHDPPQVLVIGQGCYGRMDVPPKTRRYFQEAGVEVIAATTAHASETY
                     NRLREVRRVVAALHLTC"
     gene            978..>1555
                     /locus_tag="AC16V7_02320"
     CDS             978..>1555
                     /locus_tag="AC16V7_02320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012350822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HIR6255981.1"
                     /translation="MMFKQVIRILSILMVSSTIGLGSCIAPQRLPEFLATPSAGVNNE
                     VTHTPTSQPQLIELPTPRQEGSLSFEQVLAKRRSVRQFSDDPLTLEEIGQLLWAAQGI
                     THPAGLRSAPSAGALYPLEVYAVTQQAVYHYLPQDHCLSVQVQGDVHDALYEAALQQG
                     AVREAPAVIVIAAVYARTVKKYGEERSPRYVHL"
BASE COUNT          383 a          410 c          422 g          340 t
ORIGIN      
        1 cgcattagac tcatatccct ccacctggat gcaggggagt cgaatcaaga cgcgtccatc
       61 tttaatttta acaggatagc gtggcaagtt ttgggtagct ggtccccgca atggccggcc
      121 aaacaagtca agtgaagcag gattttagaa taggtttttg gtaatttcga acctattgcg
      181 tgctatgatc caaagctcct cgcctctcgt cgaggcaatt catgaaattt tgcccatgat
      241 cttataaaaa cattgacttt ccacaaatcg atacctataa tctcctatgc aagcttccgg
      301 agcatgcgaa aggaaggtca agcatgaacc cgaaagaagc cattgccttt gatgccgatg
      361 agttgctgga gctggagatg atcctggtgg atggggacgc acaggcagcc ctcgagttcc
      421 tcaagcgagc tgtatggaag aagatccagc ttgccagaaa agggcgcttg aaatgccact
      481 tggattatgg aaccacggac actgtcgcag acttcgaaaa aaaggagaga acatgaacgc
      541 gccggtggtt cccaagatcg ataactatcg cttcggcaac attgtcatcg acgggaaaca
      601 attcacgagc gatgtgatca tctatcctga ccgggtcgag ggatcctggt ggcgggaaga
      661 gggtcactcg ttagctctgt cggatctgcc cgcagtgcta cacgatcctc cacaagtgct
      721 agtcattgga caggggtgtt acggtcggat ggacgtacca ccaaaaaccc gccgctattt
      781 tcaagaggca ggcgtcgagg tcattgctgc gacaaccgct catgccagtg aaacctacaa
      841 ccgtctgcgt gaggtaaggc gcgtggtcgc cgcactgcac ctgacctgct gaggtaaact
      901 ggcatgtgat gcaatcccat ctcagaaatg gtcaggaggt gggtcaaagg ttctttgata
      961 agaaatagat cggggggatg atgttcaaac aagtcatccg aatattgtca atcctgatgg
     1021 tctcctcgac catcgggttg ggctcttgca ttgcgccaca gcgccttccc gagttcctgg
     1081 cgacaccgtc tgctggcgtg aataatgaag tcactcacac accaaccagc caacctcagc
     1141 tcattgagct gcctactccc aggcaggagg gaagccttag cttcgagcaa gtcctggcaa
     1201 agcgacgctc tgtgcgccag ttctccgacg atccactcac attggaagag ataggacagt
     1261 tgctgtgggc tgcacaaggc atcacccacc ctgccggctt gcgcagcgcc ccatcggcag
     1321 gcgcgcttta cccgctggag gtctatgcag tcacgcagca ggctgtctat cactacctgc
     1381 cacaagatca ttgcctgagc gttcaagtcc agggtgatgt gcacgacgcg ctatatgagg
     1441 ctgccttgca gcaaggggct gtgcgcgagg cgccggctgt gattgtaatc gctgcagtat
     1501 atgcccggac ggtaaaaaag tacggcgaag aacgcagccc tcgctatgtc cacct
//
LOCUS       DBNHOH010000127         4050 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_647853, whole genome shotgun sequence.
ACCESSION   DBNHOH010000127 DBNHOH010000000
VERSION     DBNHOH010000127.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_647853"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="AC16V7_02325"
     CDS             <1..543
                     /locus_tag="AC16V7_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HIR6255982.1"
                     /translation="GMEFLAAPYLTRQQGGFYTDKEAARARIEHLEDCLLFWPYMAVV
                     DAFQHWAYTHPEAAADPAQCDACWDGLWQRFMPGVDWSSLESQRITGWHRKQHIYLDA
                     FYYVEYGLAQLGAVQVWGKALKDQVNAVAAYRKALALGNTVPLPDLFATAGAKFAFDA
                     GTLGEAVALIERVIGELEAV"
     gene            complement(570..1130)
                     /locus_tag="AC16V7_02330"
     CDS             complement(570..1130)
                     /locus_tag="AC16V7_02330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HIR6255983.1"
                     /translation="MENDTLRQAFRSLNKFIMVPLFRLGLGALVGNPLTGYIMVIQTV
                     GRKTGRLRYTPVNYALMDGYVYCMSGWGAKADWHRNLMTNPRLEVILPGGNLSGAAEV
                     VAEPDEWLRAARQLLKNAGLAGFLSGVNPFTASDEQIRQKYQGYPIMRIRPTGIGSGP
                     ADPGGWFWLLTTTLAFLWVWRKSRRK"
     gene            1236..2246
                     /locus_tag="AC16V7_02335"
     CDS             1236..2246
                     /locus_tag="AC16V7_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR6255984.1"
                     /translation="MDQTLKIVIPMAGLGTRLRPHTWSKPKQLIGVAGKPVLDHVLDT
                     LSTLPDPENIELINIVGYRGEQIEAHLGARYPHLKAHYVLQEDPRGQSHAIYLAKEYL
                     KGPMLVIFADTLIETDLSFLASEPAEAVVWVKPVPDPRRFGVAVVGDDGWVDRLIEKP
                     QEMTNNLAVVGFYYIRSAEDLVSAIEEQMKRNMQLKGEFFLADAINIMLERGLRMRTQ
                     KVDIWLDAGTPEALLETNRYLLENGRNNSAQLADREGIVVIPPVFIHPTAEVIHSIVG
                     PNVSLGAGCRVERSILSDSILEDEAQVLDVILEKSLVGRRAHIQRRAGVISAGDQTVV
                     IL"
     gene            2288..2500
                     /locus_tag="AC16V7_02340"
     CDS             2288..2500
                     /locus_tag="AC16V7_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255985.1"
                     /translation="MVDLVECHSEVTYAERPTALIWQGQRLPVDEVLARWRVPGAVRF
                     RVRTEGGLVFELEYDEVKDIWQVEER"
     gene            2512..3705
                     /locus_tag="AC16V7_02345"
     CDS             2512..3705
                     /locus_tag="AC16V7_02345"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR6255986.1"
                     /translation="MTTIAPSKGADLGKKYLATRVANLKPSGIRKFFDIVATMQDVIS
                     LGIGEPDFDTPKPILEAGLRSLQDGHTHYTSNAGQLELRQALCEHLYSLYEVKYDPAD
                     LVITVGVSEALYLAMTALLNPGDEVIVPTPCFVAYQAEVILAGGVAVELPTAAQDGFQ
                     LRPDALKAAITPRTKAILLGYPNNPTGAVASRETLLEIARIAEEHDLVVVSDELYDQL
                     VYGVSHVCFAALPGMQDRTILLGGFSKNYAMTGWRVGFAAGPADIIKGLLRVHQYTIM
                     SAPTTAQEAALEAIKNGQPHVEEMRREYDRRRKLIVTGLNELGLATVEPHGAFYAFPS
                     IAMTGMDEETFARKLLEEESVAVVPGTAFGPGGEGYVRCSYATAYEKIEEALHRIERF
                     TLRHG"
     gene            3863..>4050
                     /locus_tag="AC16V7_02350"
     CDS             3863..>4050
                     /locus_tag="AC16V7_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR6255987.1"
                     /translation="MLGYEAVIGLEVHAELETRSKMFCSCARVDSTTAQPNSAVCPVC
                     AGMPGVLPVVNRRAVEYAL"
BASE COUNT          876 a         1143 c         1163 g          868 t
ORIGIN      
        1 ggcatggagt ttttggccgc gccatatctg acccgccagc aaggtggttt ctacaccgat
       61 aaagaagccg cccgcgcccg catcgagcac ctggaggact gtctattatt ctggccttat
      121 atggctgtgg tggacgcgtt ccagcattgg gcatacacgc atccagaagc cgccgccgac
      181 cctgcccagt gcgacgcctg ctgggatggg ctgtggcagc gtttcatgcc cggcgtggac
      241 tggagtagcc tggagagtca gcgcatcacg ggctggcatc gcaagcagca catttacctg
      301 gatgcgttct attatgtgga atatgggctg gcacaattgg gcgcggtgca ggtctggggc
      361 aaggctttaa aagatcaggt caacgcggtg gcagcatacc gcaaggcgct cgccttgggg
      421 aatacggttc cgctacctga tttatttgcc accgcggggg ctaagttcgc ctttgatgcg
      481 ggcacgctgg gcgaggcggt ggctttgatt gaaagggtta ttggcgagct agaggcggtt
      541 tgaccatctc tctggggttg ccttcgtccc tacttgcgac gagacttcct ccacacccag
      601 agaaacgcca gagtggtcgt caggagccaa aaccagccac ctggatcggc ggggccactg
      661 ccaatacctg tcgggcggat gcgcatgatt gggtacccct ggtatttctg gcggatctgc
      721 tcgtcgctgg cggtgaaggg gttcacgccc gataaaaaac cagccaagcc agcgttcttc
      781 aaaagctgtc gggcagctcg cagccattcg tctggttcag ccactacttc agccgcgccg
      841 ctcaggttac cccctggtaa aatcacctcc aggcgcgggt tggtcatcaa attacgatgc
      901 cagtcggcct tcgcccccca tcccgacatg cagtaaacgt agccatccat caaggcgtag
      961 ttaactgggg tgtaacggag gcgccccgtc ttgcggccta ccgtctggat gaccatgatg
     1021 tacccggtaa gcggattgcc aaccagcgcc cctagcccca aacggaagag gggtaccata
     1081 ataaatttgt taagcgagcg gaaggcctgg cgtaaggtat cgttttccat atcaggttat
     1141 aatatttgcg caacgcaact tgtttgaaat tgtaatgatg gtagaaacga aattactcag
     1201 aactttatac ttattatagg ataaacggca tttttatgga tcaaacacta aagatcgtca
     1261 tccccatggc tgggctgggc acccgcctgc gcccgcatac ctggagcaaa cccaagcaat
     1321 tgatcggcgt ggcgggcaag cccgtcctgg atcatgtgct ggacacctta tccaccctcc
     1381 ccgacccaga aaatatcgag ctgatcaaca tcgttggcta tcgcggcgag caaattgagg
     1441 ctcatctcgg ggcgcgctat ccccacttga aggcacatta cgtgctgcaa gaagacccgc
     1501 gcggacagtc gcacgccata tatttggcga aggaatacct caaaggtccc atgctggtga
     1561 tctttgccga caccctgatt gagaccgatc tatctttcct ggccagcgag ccagcggagg
     1621 cggtggtatg ggtaaaaccc gtgcccgacc cgcgccgctt tggcgtggct gtggtgggcg
     1681 acgatggttg ggtcgaccgc ttgatcgaga agccgcaaga aatgaccaac aacctggccg
     1741 tggtgggttt ttattacatc cgctccgccg aggacttggt gtccgccatc gaagagcaga
     1801 tgaagcgcaa catgcagctc aaaggcgagt tcttcctggc cgatgccatc aacattatgc
     1861 tagagcgcgg cttgagaatg cgcacacaga aggtggatat ctggttagat gccgggacgc
     1921 cagaggcgct gctggagacc aaccgttatc tgctggagaa tgggcgcaac aattccgccc
     1981 aacttgccga ccgcgagggc attgtggtta tcccgccagt gttcatccat cccactgccg
     2041 aggtgatcca ctccatcgtc ggaccaaatg tttcattagg tgcaggttgc cgcgtggaac
     2101 gcagcatcct ctcagattcg atcctggaag acgaagccca agtgttggat gttatcctgg
     2161 aaaaatctct ggtgggtcgc cgtgcgcaca tccagcgccg cgctggcgtg atcagtgctg
     2221 gcgaccagac cgtggtcatc ttataattac acaagttaca ccttcgggaa gtacgcaagc
     2281 ccttaatatg gtagatttgg ttgaatgtca ttctgaagtg acttacgccg agcgaccgac
     2341 cgccctgatc tggcaagggc aacgcctacc cgtggacgaa gtgctggcac gctggcgagt
     2401 tcccggggca gtgcgtttcc gcgtccgcac cgagggcggt ctggtcttcg agctggaata
     2461 tgatgaagtc aaagatatct ggcaagttga ggaacgatag gatgaagtct aatgaccacc
     2521 attgcgccat ctaaaggagc cgatttgggc aaaaaatacc tcgccacccg cgtcgcaaac
     2581 ttaaaaccct ccggcatccg caaatttttt gacattgtcg ccaccatgca agatgtgatc
     2641 tcgctgggca ttggcgaacc ggattttgac acacctaagc caatcttaga agctggcttg
     2701 cgctccctgc aggacgggca cacgcactac acctccaatg ccgggcaatt ggagctacgc
     2761 caggcgctgt gtgaacacct gtacagcttg tatgaggtga aatatgaccc ggccgatctg
     2821 gtcatcacgg tgggcgtctc ggaggcgctc tatctggcaa tgacagccct gctcaatcct
     2881 ggcgacgaag tcatcgtacc aaccccatgc tttgtcgctt accaggcaga ggtgatcctg
     2941 gcaggcggcg tggctgtgga gcttcccaca gcagcacaag atggcttcca gttgcgcccc
     3001 gatgccctga aggcagccat cacgccccgc acgaaggcca tcttgcttgg ctaccccaac
     3061 aatcccaccg gcgcggtcgc tagtagggag acattgcttg aaatagcccg catcgccgag
     3121 gaacatgacc tggttgtggt atctgacgaa ttgtatgacc agcttgtcta tggtgtgtct
     3181 cacgtctgct ttgctgccct gcccggcatg caggaccgca cgatattgct gggcggtttc
     3241 tccaaaaact atgccatgac cggctggcgc gtcggtttcg ccgctggacc ggcggatatc
     3301 atcaaaggtc tcttgcgcgt gcatcagtac acgatcatgt ctgcgcccac cactgctcag
     3361 gaggctgcac tggaagccat aaagaatggt cagccacatg tggaagagat gcgccgcgag
     3421 tacgaccggc ggcgtaagct gatcgtcaca ggcttgaatg aattggggct ggcgactgtc
     3481 gagccacacg gcgcattcta cgcctttccg agtatcgcta tgaccgggat ggacgaggag
     3541 accttcgccc gcaaattact ggaagaggag agcgtggctg tcgtccctgg cacagccttt
     3601 ggaccaggcg gggaggggta tgtgcgctgt tcctatgcca cagcttacga gaagatcgaa
     3661 gaggccttgc accgtatcga gcgcttcacg ctccgccatg gatagagatg aaaactcccg
     3721 ggctcattga tatatgccgt ggtcagagcc ggatgtgcgg tgtgtgagca gttcatgacc
     3781 cgcccgcact caccatactt cccctgtgcc acgcaagttc ccataacccc ggattctagt
     3841 tcagtatgac ctgcggataa gcatgttggg ttacgaagcc gtcatcgggc tggaagtgca
     3901 cgccgagcta gaaacgcgct ccaagatgtt ctgctcttgc gcgcgggtcg attcaaccac
     3961 cgcacagccc aactccgcag tgtgccccgt ctgcgctggt atgcccggtg tattgccggt
     4021 tgtaaatcgc cgcgccgtgg agtatgccct
//
LOCUS       DBNHOH010000128         2895 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_134819, whole genome shotgun sequence.
ACCESSION   DBNHOH010000128 DBNHOH010000000
VERSION     DBNHOH010000128.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_134819"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(291..800)
                     /locus_tag="AC16V7_02355"
     CDS             complement(291..800)
                     /locus_tag="AC16V7_02355"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6255988.1"
                     /translation="MSTSEIPAEKAVKYCQRCGASMTHQQRYGRERPVCPRCEWIFFP
                     DPKVASAVLVEQDGKILLVRRALDPEKGKWTLPAGFIDAGEDPARAAERECLEETGLR
                     VQVKELLTVLYGQEHPHGAHIIIFYRAELVSGSLRPGDDVDAVEFFPPNQLPPLAFQT
                     THKVLSQKN"
     gene            complement(811..1683)
                     /locus_tag="AC16V7_02360"
     CDS             complement(811..1683)
                     /locus_tag="AC16V7_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR6255989.1"
                     /translation="MTTNLTPRDWEALSAYLDGQLAPKEQQLLEARMRSRADLRAAME
                     ELRRTRAVLRAHLEVRAPRNFTLTPEMAGVRPRSRPTVAFFPALRLTSAISSLLFVLV
                     ALSELLIGGRLALPMAAGEPQREAMSEAAPQEPALEPSAAPAMAVEAPAAEEVEATPQ
                     PVAKAVEAPTPSLGLELAQPIVIETETVELPPFAGGMGGGDGADETQLDEEALSQMAP
                     IATATPLPTLTFTPQPSPSPTVMEQPTAPAPDRARFARFPEWTVLRWVEITLAIVGLS
                     TGLLAIILHRRGRS"
     gene            complement(1680..2288)
                     /locus_tag="AC16V7_02365"
     CDS             complement(1680..2288)
                     /locus_tag="AC16V7_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6255990.1"
                     /translation="MDEAALISDARQGDLNAFNRLILAYQDLVYAQAYRMMGEPEAAE
                     DATQEAFISAFRSIRSYRGGSFRAWLLRIVTNACYDELRRRKRRPTTPLEPMDAEDEE
                     IESPHWLADPSESPEDRTERLELDQAIQNCLEDLPPDFRSVVVLVDIQGFDYIEAAEV
                     IKKPVGTVKSRLSRARLRLRDCLQGWWELIPAQFRLVNEGKP"
     gene            complement(2293..2676)
                     /locus_tag="AC16V7_02370"
     CDS             complement(2293..2676)
                     /locus_tag="AC16V7_02370"
                     /inference="COORDINATES: protein motif:HMM:NF022700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3054 domain-containing protein"
                     /protein_id="HIR6255991.1"
                     /translation="MKDNRLLGVLAGDVITLALVTLAGFARHDELDSANFRMLTTFIP
                     LVLAWLLVAPHLAVFDLQRLSDPRQLWRPFWAMVLGAPFAGWLRGVMLNSPVLPIFVF
                     VLGGVSALALLAWRSAYWLIFARRS"
BASE COUNT          722 a          808 c          756 g          609 t
ORIGIN      
        1 agacctaaca caaacaggtg cttgaatctc cacatcttca ttgcttcttc tccctataag
       61 cgaattgaac caggaaaact aattactcct agctaaatat aggcgtagtt taccataacc
      121 gcactgccca acacgcatat agaaactgcg cctattaaat ttgtaaagca acgccagact
      181 ttatcacttc ctggactcat ctcaagctat actggtattg acaccaaccc acacacgagc
      241 agtttaatgt ttctgcatat tcgtcttagg atgaaaacag ccagcatctg tcagtttttt
      301 tggcttaata ctttatgggt ggtctgaaaa gccaaaggag gtaactgatt aggtggaaag
      361 aattccacgg catctacatc gtctccgggg cgcagcgagc ccgagaccaa ctccgcccgg
      421 tagaagatga tgatgtgagc accgtgtgga tgttcttgac cgtaaagcac ggtgagcagt
      481 tcttttacct gcactcgcag acctgtctct tccaggcact cacgttcggc ggcacgagcc
      541 gggtcttcac cggcatcgat gaagcctgcc ggcaatgtcc actttccttt ttctgggtca
      601 agggcgcgcc gcacgagcag aatctttcca tcctgctcta caagaactgc agaagctacc
      661 tttgggtcag ggaagaagat ccattcacaa cgtggacaaa ccgggcgctc acggccgtaa
      721 cgttgttggt gggtcataga tgccccgcag cgctggcaat acttaaccgc tttctcagca
      781 ggtatctcgc tggtactcac aagatttcct tcatgaacgt ccacgtcgat gaaggataat
      841 ggccagcaaa cctgtgctca aacccacgat agccagggtg atttcaaccc agcgcaaaac
      901 cgtccattct gggaaacgag caaaacgagc cctgtccggg gctggcgcgg tgggctgttc
      961 cataactgtt ggggacggcg aaggttgtgg ggtaaaggta agtgtgggca agggtgtagc
     1021 agtcgctata ggagccattt gcgagagcgc ctcttcgtct aattgggttt catcggctcc
     1081 gtctccgccg cccataccgc ctgcaaatgg aggcaactcg acggtctcag tctcgataac
     1141 aatgggctgg gcgagttcca gtccaaggct gggagtaggg gcttccacag ccttggcgac
     1201 tggctgaggc gttgcttcaa cttcttcagc tgctggagct tctacagcca tcgctggagc
     1261 tgccgagggt tcaagagcag gttcttgagg tgcggcttca gacatcgctt cacgctgcgg
     1321 ctcaccagcc gccatgggta aagccagacg acccccaatc agtagctcac tcaatgcgac
     1381 gaggacaaac agcaagctag atattgccga agtcaggcgc aacgctggaa agaaggccac
     1441 cgtcgggcgg ctgcgaggtc ggacaccggc catctcgggt gtcagggtga aattacgagg
     1501 ggcgcgcacc tctagatgcg ctcgcaatac cgctcgagtc cggcgcagtt cctccatggc
     1561 agcgcgtaaa tctgccctcg atcgcatcct ggcttcgaga agctgctgtt ccttcggggc
     1621 cagttgccca tccaggtaag ccgataaggc ttcccagtca cgaggggtaa gattagtcgt
     1681 catggtttac cttcattcac cagacgaaat tgggcgggaa taagttccca ccaaccttgc
     1741 aaacagtcgc gtaaacgtaa gcgtgcacgc gagagacggc tcttcaccgt ccccacaggc
     1801 tttttaatca cctcagcggc ctcaatataa tcaaatccct gtatatccac caatacaacc
     1861 accgaacgga aatccggggg taagtcctcc aggcaatttt ggatggcttg atccaattcc
     1921 aggcgctcag ttcgatcctc cggcgattcg ctggggtcag ctaaccaatg tggcgattca
     1981 atttcttcat cctcagcatc cattggctcc aggggagtcg tcgggcggcg cttacgtcgg
     2041 cgcaactcat cataacaggc attggtgaca atgcgcagca accaggctcg gaacgagccg
     2101 ccgcggtaag agcggatact gcggaaagcc gagataaatg cttcctgggt ggcgtcctcg
     2161 gcggcttctg gctcacccat catgcggtat gcttgggcgt agaccagatc ctggtatgcc
     2221 agtatcaggc ggttgaaagc attgagatca ccctgcctgg catctgagat gagcgcggct
     2281 tcatccatgc aatcagctcc gccgagcaaa aatcaaccaa tatgcgctgc gccaggcaag
     2341 caatgccagg gcgctcactc cacctaggac gaacacaaat atgggtaaga ccggtgaatt
     2401 caacataacg cctcgcagcc atcccgcaaa gggtgccccc agaaccattg cccagaaggg
     2461 tcgccataac tgcctggggt cgctgaggcg ctgtaggtca aaaactgcca ggtggggcgc
     2521 taccagcagc caggcaagca caagcgggat gaaagtggtc agcatgcgaa aattagcgct
     2581 gtctaactca tcgtgcctgg caaaacctgc cagtgttacc aatgccaggg tgatcacatc
     2641 cccagctaat acacccagca gacggttatc cttcatgaat catatcctaa cccaaagcga
     2701 tcaaagggac aagggagatg cgctactaat acaatgacca taagcgaggc accagcagaa
     2761 ttgccacaat atatatgaac acggtcagca acgatcccac tttgagaaag tcggtgaaac
     2821 gatagcgccc caacccatat acaatcaaac acgcgggttc cagcggggtg atatacgatg
     2881 tgctggcagc cactg
//
LOCUS       DBNHOH010000129         3884 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1417686, whole genome shotgun sequence.
ACCESSION   DBNHOH010000129 DBNHOH010000000
VERSION     DBNHOH010000129.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1417686"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="AC16V7_02375"
     CDS             <1..675
                     /locus_tag="AC16V7_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255992.1"
                     /translation="DFKLNRESRPSLALVALWLFAPLVGAIVVMPTMYDNFRQFLFMT
                     PPLFIFAGLGVQKLFLYAQRLGRLSSPDRKPNALSPFLRSAAKILILAIALSPGLYWD
                     VVLHPYQYVFYNQLVGGVSGAYRRYEMDYWTTSYKEASEYLNARAPDGARVVVYGADH
                     IVKSYARKDLEISDLKRVDSRQDYAVITTRHDKDLELYPSAPIVFQVGRDGAIFTVIK
                     RLSPQE"
     gene            complement(589..2550)
                     /locus_tag="AC16V7_02380"
     CDS             complement(589..2550)
                     /locus_tag="AC16V7_02380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR6255993.1"
                     /translation="MKFLRISVPSFKSKTNLLLLVGVMIQGALILGISWRELNGVRKT
                     LGMPALWRGANFSQGGKFADYTLFLNENIPATGRVVLPPAGYGPRALGTTPYMQFFLA
                     PREVINCTDLACAQNLSPENTYLLVVEGFPGDEAAKRFGEQRMFNDKWGVLSPSSAPP
                     GQSAHLGFNRLVDILLAALLPLLWLGTLAFSGYLLVTSLAPNLPSAMHLGLGYALGLG
                     LLTAGLGLAWLAGASLMGIVTVVVTALLLVVSMVVYFLRGRDASSQSARLTLRPAGLP
                     DPWVLAFLFLSGGAAVLSVGQGYSVSDEIVLWGVKGYGIAATGSLANIQDWGTNTTIY
                     PLHVPILIATFKFLFAETLPAAKLAFSGYYLALLLFSYHFLTWKGVTRSRAGLATLLL
                     ATVPLAFRHATIAYANLPLAFSLLCGVALLSGALQERTALHPMRAYILAGLLLSMAAW
                     TRPEGLGMAVLCALALFLYDYLRWRKPPSIKKLAVFAAPLLAYGLFWVTLKARAYTQP
                     SGRSGLLGNALQAVSSGQWHLGEALYILRSLAFSLIDPLTWGVFGLVLLLFALLSLRK
                     GYRRHAQAAQLALTGVICLGAVLGMYYITSYDTNHDISWWVSTGLERMLLPAASLLWL
                     GLFLGGQPLDHGEDRPIPADLEYDRRAGI"
     gene            3258..>3884
                     /locus_tag="AC16V7_02385"
     CDS             3258..>3884
                     /locus_tag="AC16V7_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR6255994.1"
                     /translation="MDKPIPEIKLESWLISAGRNSEPGAPLNVPLVPASNFIIGKGRE
                     YSRSDGTPTWEALEEIVGGLESGKAVAFASGMAAIASVFDQLAADAVIVLPDDCYLGV
                     SSLAAAGVERQRWSVQHVAVDDTDGWIRACGIADLIWLESPSNPLLTVADLEAICAAP
                     RKPGAVIAVDNTFATPMNQQPLDFGATVSLQSATKFIGGHSDLLAGVAT"
BASE COUNT          956 a         1110 c         1009 g          809 t
ORIGIN      
        1 gacttcaaat taaacaggga gagccgccca tcgctggcac ttgttgccct atggctattt
       61 gcgccgctcg ttggtgccat cgtagttatg ccaaccatgt acgataactt ccgacagttc
      121 ttatttatga caccaccgct attcatattt gcagggttag gggtacaaaa acttttcctt
      181 tatgcacaac gtctcggaag gttgtcatcg ccagaccgca agccgaatgc actctctccg
      241 ttcctccggt ccgcagcaaa aatattgatc ctggctatcg cgttgtctcc cggcctctac
      301 tgggacgtgg tcctgcaccc ctaccagtat gtattttata accagcttgt aggcggcgtg
      361 agcggtgcat accgccgtta cgagatggat tactggacaa cttcatacaa agaggcaagc
      421 gaatatttaa acgcccgtgc ccctgatggc gcccgggtcg tggtgtacgg cgccgaccac
      481 atcgtcaaga gctatgcccg gaaagacctg gagatcagcg acctcaaaag ggtggattcc
      541 cgccaagatt atgcggtgat taccacccgt cacgacaagg atttggagct atatcccagc
      601 gcgcctatcg tattccaggt cggcagggat ggggcgatct tcaccgtgat caagcggctg
      661 tccccccagg aataacccta accatagcag gcttgcggct ggcagcagca tgcgctccag
      721 gcccgtgctc acccaccagc tgatatcgtg atttgtatca taagaggtga tgtagtacat
      781 gcccagcacg gctcccaggc agatcacacc agtaagagca agctgcgccg cctgtgcatg
      841 ccgtcggtag cctttacgca ggcttagtag ggcaaaaagc aacagaacaa ggccgaatac
      901 accccaggta agcggatcaa tcagactaaa cgccagggag cgcaaaatgt atagcgcttc
      961 gcccaggtgc cattgacccg acgacaccgc ctgcaaggcg ttacccagca gtccagagcg
     1021 acctgatggc tgcgtgtagg ctctcgcttt cagagtaacc caaaacagcc catacgccag
     1081 cagcggcgcg gcgaaaaccg cgagcttttt tatcgatggg ggttttcgcc accttagata
     1141 gtcatacaag aacagcgcca gggcgcacag gacagccatc cccaacccct ccggccgcgt
     1201 ccaggctgcc atgctcaaaa gcaacccggc gaggatgtaa gctcgcatcg ggtgaagtgc
     1261 ggtcctctct tggagcgctc cactcagcaa ggcgacccca cacagcagag aaaaagcgag
     1321 cggtaggttg gcgtaggcaa tcgtggcatg gcggaaggca agcgggaccg ttgccagcag
     1381 caaggtcgcc agtccagccc ggctgcgggt tactcctttc caggtcaaaa aatggtacga
     1441 aaagagcaac agggcgagat aataaccgct gaaggcaagc ttagccgcag gcaacgtttc
     1501 agcgaagagg aacttgaagg ttgcaatcag gatggggacg tgcagggggt agatggttgt
     1561 atttgtaccc caatcctgga tattcgccag cgaccctgtg gcagcaatgc catacccctt
     1621 tacaccccac agcacgattt catctgaaac agaatatccc tgcccaactg ataaaaccgc
     1681 agcgcccccc gataggaaca ggaaagctaa cacccaggga tcaggtaagc cggctgggcg
     1741 gagggtcaac ctggcagact gagaagatgc atccctgccc cgtaagaagt aaacaaccat
     1801 cgagacgacc aacagcaggg cagttaccac cacagttacg atacccatca aggaagcccc
     1861 ggcaagccaa gccagaccga gccccgcggt cagtaagcct agccccaaag cgtaaccgag
     1921 cccaaggtgc atcgcactcg gcaagttggg ggctagggac gtcaccagca gataaccact
     1981 aaaagccagc gtacccaacc acagcaaggg cagcagggct gccaggagga tatccaccag
     2041 ccggttaaag ccaagatgtg ctgactgccc gggcggcgcg ctactcggtg acaacacccc
     2101 ccacttgtca ttgaacatgc gctgctcccc gaatctcttt gcggcctcgt ctccaggaaa
     2161 accctccacg accagcaggt aggtattttc gggcgaaagg ttctgggcgc acgctagatc
     2221 ggtgcagttg atcacctctc gcggagccag gaagaattgc atataaggtg tggtccccag
     2281 cgctctcggc ccgtaaccgg caggcggtag cacaacccga cctgtggcag ggatattctc
     2341 attcagaaac agagtgtagt cagcaaactt tcccccctgg ctgaaatttg cgcctcgcca
     2401 caaggcaggc atacccagcg ttttgcgcac gccgttcaac tccctccacg atatacccag
     2461 gatcagcgcc ccttgaatca taaccccgac cagcagcaac aggttggtct tcgacttgaa
     2521 acttggcact gaaatgcgaa gaaatttcac aacttatcct gaaagcacgc acaaaccaga
     2581 tgaagatagg ctattatttc tggttttctg caaccctcgc ctttaccatt ttgccgatta
     2641 attcaaatgt attcatcaat cgtggtcgga tgacctcttt tctcttccat gacatatgcc
     2701 tttgctggtt ttagcctgcc tgtaaccttg aattataaag tattcctatg atgtaatccg
     2761 cgcctgtatg cttctatagg gcaatcgcat ttgggcgatt tcccaccact gaggagagaa
     2821 aatggggtgt tttggtgggg gcatatgcaa ttgccctgat gcatctggaa cacgatcaat
     2881 tccaagcagt actgtgattg gccaggctcg ctccacttgt gctttcccat gccatagcaa
     2941 cgaccctaga gacatgctgg ctaggttacc ccttgcgttt aaggtggagg ccacatccat
     3001 tttcgcgaag agaacctgag atagaccacg cctcccttcc agagtaaaga agcaggcgac
     3061 cgttggatcg atctgagcgg caacgcctga tcgcgaaata ccagaaagac agatcaagtt
     3121 ttaacctaat gccgcatata atcatatata tggttcgcag aatcactcgt ggctgcacca
     3181 ctcaatccgc ggcataactc ccgtcgggcc agacagatcc gtagcagaaa tcttcttgtg
     3241 tggaggtatt cgatacaatg gataaaccaa taccagagat caagcttgaa tcgtggctta
     3301 tctcagcagg tcgcaattcg gaacccggcg ctccgctaaa cgttccgctg gttcccgcat
     3361 cgaatttcat catcggcaag gggcgcgaat actcgcgctc tgatggtacg cccacctggg
     3421 aagcgcttga agagatcgtt ggcggacttg agtctggcaa ggcggtggct tttgcctccg
     3481 gcatggccgc catcgcctcg gtgttcgatc agcttgcagc tgatgctgtt attgtattac
     3541 cagatgactg ctacctgggc gtttcaagtc tagcagcagc aggcgttgaa aggcagcgct
     3601 ggtcagtgca gcacgtggca gtcgatgata cggacggctg gattcgcgcc tgtggcatcg
     3661 cagaccttat ttggctcgag tcaccctcga acccactgct taccgtagca gatcttgaag
     3721 ccatctgcgc agcaccacgc aagccagggg ctgttatagc ggtggacaat acttttgcca
     3781 ccccaatgaa tcagcaaccg cttgactttg gagccacggt ttccctccag tcggcaacaa
     3841 agtttattgg tggtcactct gatttactgg caggggtcgc cacg
//
LOCUS       DBNHOH010000130         1621 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1480978, whole genome shotgun sequence.
ACCESSION   DBNHOH010000130 DBNHOH010000000
VERSION     DBNHOH010000130.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1480978"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="AC16V7_02390"
     CDS             <1..505
                     /locus_tag="AC16V7_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR6255995.1"
                     /translation="NGAPLRDGQRVAVDSSGLEQAEFLIAPNPGEGLKPLVKIASGGE
                     TSRLMLALKHVLTSADQIPTLIFDEIDQGIGGRVGAVVGQKLWRLAQKHQVLCVTHLP
                     QLAAFGTQHFHVEKLVQEGRTMTRVSQLTGNLRVEELAQMLGGISEGTLQSAREILQA
                     VDNQTTH"
     gene            512..>1621
                     /locus_tag="AC16V7_02395"
     CDS             512..>1621
                     /locus_tag="AC16V7_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013106.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR6255996.1"
                     /translation="MPSPAQITSLRRHRHNRAGRYSKTWFGVGCSTFLSLFAVLVGMM
                     GVWGYITLFHNLPSLDTLPALLDPPDGLLLQPTRLYDRTGTHVLLELENPAAVDRQVL
                     TLQSNQPNYLPSSLISATLATADPQFWSHAGYSFDGLTSGTHPTLAQTLVSDLLLWSE
                     PASLRRAVRERLLAAQITARFGRQQVLIWYLNSANYGRLAFGADVAARVYFNKPAAEI
                     NLAEAAVLAAVMQSPALNPLDAPNVVRERAVDVIQTMLEGGWINAAQASQARKSNLVF
                     RTQTETLANPAPIFINLVLEQLGQELSLNRLERGGFQIVTTLDQDLQFQAACALQLFT
                     ARLQGMPDPPNTMNGDECRSARLLPTSFTGQSMTVK"
BASE COUNT          374 a          468 c          426 g          353 t
ORIGIN      
        1 aaacggcgca cccctccgtg atggacaacg cgtagcggtc gactcgagcg ggctggagca
       61 ggcagaattt ctcatcgccc ccaacccagg cgagggtttg aaacccctgg taaagatcgc
      121 ctccggaggc gaaacctcac gcctgatgct agcccttaaa catgttctta ccagcgccga
      181 tcaaatcccc accctcatct ttgatgaaat tgaccaaggc atcggcgggc gtgtgggcgc
      241 agtggtcggc cagaaacttt ggcggttggc acaaaaacat caggtgctgt gtgtaaccca
      301 cctgccacaa cttgctgcct ttggcactca gcacttccac gtggagaagc tggttcagga
      361 agggcgcaca atgacccgtg tttcccagct aaccggaaat ctccgggtag aagaactggc
      421 acagatgctg ggtgggataa gcgagggcac cttgcaatcc gctcgcgaga ttctccaggc
      481 ggttgacaat caaaccacgc actgagaaag tatgccctct ccagcgcaga tcacatccct
      541 tcgcagacat cggcacaatc gggcaggtcg atactcaaaa acctggtttg gggtaggttg
      601 cagtacattc cttagtttat tcgcggtgct ggtagggatg atgggggtgt ggggatatat
      661 tacacttttt cataatttac cctcactgga tacgctgcct gccctgttag acccgccaga
      721 cgggttgctg ctccagccaa cccgcctata tgaccgcacc ggcacacatg tcttattaga
      781 attggaaaac cctgctgcgg ttgatcgcca agtactcacc ctgcaaagca accagccaaa
      841 ttatctgccc tcgagtttga tctctgccac actggcaacc gccgatccgc aattctggag
      901 ccatgcaggc tattcttttg atggcttgac ctcgggcacc caccccacac tcgcccaaac
      961 tctggtttct gatttgctgc tgtggagcga gccagccagc ctacggcgag cagtacggga
     1021 gcgtctgctg gcagcccaga tcacggcgcg ttttggtcgc caacaggtgc tcatctggta
     1081 tctcaacagc gctaactatg gccggctggc atttggcgca gatgttgccg cgagggtcta
     1141 ttttaataag ccagcagccg aaatcaactt agctgaagca gccgtactgg ctgcagtaat
     1201 gcaatcaccc gctttgaacc cgttggatgc gcctaatgtg gtcagggaga gagcagtaga
     1261 cgtgatccaa accatgttgg aaggcggctg gatcaacgct gcacaagctt ctcaggcccg
     1321 caagagcaat ctggtgtttc gcacacaaac agaaacctta gccaaccccg cgcccatttt
     1381 tatcaatctg gttttggagc agctaggcca agagcttagc ctgaatcgcc ttgagcgcgg
     1441 tggcttccaa atcgtcacta cccttgatca ggatctgcaa tttcaagctg cgtgcgcttt
     1501 gcaattgttt acagcccgac tgcagggtat gccagatccc cccaacacga tgaatggcga
     1561 tgaatgtcgg tcggcgcgac tgctgcccac atccttcact gggcaaagca tgaccgtgaa
     1621 g
//
LOCUS       DBNHOH010000131         3776 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_904634, whole genome shotgun sequence.
ACCESSION   DBNHOH010000131 DBNHOH010000000
VERSION     DBNHOH010000131.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_904634"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="AC16V7_02400"
     CDS             complement(<1..1226)
                     /locus_tag="AC16V7_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255997.1"
                     /translation="MAHVLDLDDDQSSKMLVATIKSPDSEVSTRLTSDFSNSEELRGQ
                     GYLLSLERPLTLQKDVQYQLSLALETNSGTLTLLGSAPANESTWDDGLPLRLEGYDGY
                     GGIYQTGLNFEMYWDDNADKFQRFVTTLDQADYIFISSNRQWGTIPRVPERYPLSSAY
                     YRNLLGCPPDKDIVWCYSVAQESTFQGNLGFELAKVFDSSPRIGPWVFNDQFAEEAFT
                     VYDHPKVLIFRKTSDYDPVKVRAVLGEVDYSQAVHITPRRASARPVNLTLPIERWAEQ
                     RAGGTWSELYNTRALHNRFPALGAGLWYLVLTVLGWVVYPLARLALPGLVDRGYPMAR
                     TIGLLLLSYLVWLAGSARIPFTRITISLVFALILLASAMVVYRQRGELRQEWQERRGY
                     FLRVEMIFLAFFVFDL"
     gene            complement(1205..2920)
                     /locus_tag="AC16V7_02405"
     CDS             complement(1205..2920)
                     /locus_tag="AC16V7_02405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR6255998.1"
                     /translation="MNIVPDQKSRRTLVFDLLLVCVLVIGAYFRLVGLNWGEYQYLHP
                     DERFLIWVGSDIQPVGTDPEKLGPPPSTALNPARGNHPDAFPDCQAWGGYFDASCSPL
                     NPNNRGHGFYVYGTLPMFLTRYIVEWIYGHSGFQEMTDVGRFLSAMADLLTVFLVYVI
                     ATRLYDRRVGVLSAAFSAVAVLQIQLSHYFAMETFLNLFTYLAIYFAVRVGDTKWQVG
                     EEGVLAAGEMTGITESAKRFVKHPLFIFSIAFGFALGCAVASKLNAGAVAVALPVVFG
                     LKLLAVPAEERMRRLGEAFAYLCLAAVVSLLAFRLFQPYAFSGPGFFGLKPNPMWVSN
                     ILEQRIQASGDVDFPPALQWARRPVWFSGKNLVLWGLGLPLGLLAWAGFLWAGWKLLA
                     DKSENHKELRRHGLLWGWTLVYFIWQSLEFNATMRYQFPIYPALAIFAGWALVQLWIE
                     GGKYSASAVSNISKLKLQSINIKRVLAVMVGGVVLLASIGWAWAFSRIYVRPITRIEA
                     SRWIYQNVPGPINLRIRSSENTINQPLPYPEAFAITKYQPYVTRFHQPRTVSSLRYSW
                     RMYLI"
     gene            complement(2999..>3776)
                     /locus_tag="AC16V7_02410"
     CDS             complement(2999..>3776)
                     /locus_tag="AC16V7_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6255999.1"
                     /translation="WKALSMFAWDNGEIWAISVTHRPALDVVSAVLFYLGSVFVLLRY
                     LRRRQWLDLFWLLAVPLLMLPSILSLAFPAENPALNRLSGAIVPVFLIVGLALDGFLE
                     TLKSQFGGRLGNALAVGAGLFLVIWASFQNFDIAFNQYQETYLRSSWNTSELGQVIHD
                     FAGGSGTPDTAWVIAYPHWVDTRLVAMNAGFPTRDYGIWPENLQETLPETRAKLFLLK
                     PEDEVGLSTLRQLYPDGIQSVYPSKVEGHDFLIYFVPPLK"
BASE COUNT         1064 a         1045 c          930 g          737 t
ORIGIN      
        1 aagagatcga aaacaaaaaa tgccaggaag atcatctcta cccgcaggaa atagcctcgg
       61 cgctcctgcc actcttgacg caactcaccc ctttggcgat aaaccaccat ggcgcttgcc
      121 agaaggatca aggcgaaaac caggctgata gttatgcggg taaagggaat gcgagcagaa
      181 ccagccagcc aaaccaggta actgagtagc aaaaggccaa tggtgcgcgc catcgggtag
      241 ccgcgatcaa ccagccccgg taacgccaga cgggctagcg ggtaaaccac ccaacccaaa
      301 acggtcaaga ccagatacca tagtcctgca cctagagccg ggaatcggtt gtgcagggcg
      361 cgtgtattgt acaactctga ccaggtgcct ccggcgcgtt gctctgccca acgctcaatg
      421 ggtagggtca gattaacagg ccgcgcgctt gcccgccttg gtgtgatatg aacagcttga
      481 gagtaatcaa cctcgccaag cacagcccgc accttcaccg ggtcgtaatc actggtcttg
      541 cggaagatga gcaccttggg atggtcatat accgtaaagg cctcctcagc aaactggtcg
      601 ttaaataccc agggaccaat gcgcggggat gaatcgaata ctttggcaag ctcaaagccc
      661 aaattaccct ggaaagtgct ctcttgagcc acactgtaac accaaacgat atccttatct
      721 ggtggacaac ccaatagatt gcgatagtac gcggagctca acgggtaacg ctctggaacc
      781 cggggaattg tcccccactg gcgattggag gaaataaata tgtagtcagc ctgatctaag
      841 gtagttacaa agcgctggaa tttatcggca ttatcatccc agtacatctc gaaattgaga
      901 cctgtctggt aaatgccgcc atagccatca taaccctcta agcgcagggg caaaccatca
      961 tcccaggtgc tctcgttggc aggcgcagat ccaagcaggg tgagcgtacc gctgtttgtt
     1021 tccaatgcca gggagagctg gtattggacg tctttttgca aagtcaaagg gcgctccagg
     1081 ctgagcaaat aaccctgccc gcgcagctcc tcgctattac taaaatctga ggtcaagcgg
     1141 gtggatacct cactgtcggg tgactttata gtggcgacta acatcttgct agattggtca
     1201 tcatctaaat caagtacatg cgccaagaat atctcagtga gctgaccgtc cgcggctggt
     1261 gaaaacgcgt cacgtagggt tgatatttcg tgatagcaaa ggcttcagga taaggcagtg
     1321 gctggttaat tgtgttttcg ctgcttcgga tgcgcaagtt tatcgggcca ggcacattct
     1381 ggtaaatcca gcgagatgct tcaatacggg tgatggggcg cacatagatg cgcgagaaag
     1441 cccatgccca cccaatggag gccaatagga caactccacc caccatcact gccaagacac
     1501 gttttatatt tatggattga agcttaagct tagaaatatt gctgacggcg ctggcactgt
     1561 acttgccgcc ttcaatccac aattgcacca acgcccaccc ggcaaagatc gcaagtgccg
     1621 gatatatcgg aaactgatag cgcatggtcg cattaaattc caatgattgc cagatgaaat
     1681 aaaccaaagt ccaaccccac aacaagccat ggcggcgaag ctccttgtgg ttctcgctct
     1741 tatctgctaa caatttccaa ccagcccaca ggaagccagc ccatgccaac aaccccaaag
     1801 gcaagccgag cccccaaagc accaggttct tccccgagaa ccatacaggg cggcgcgccc
     1861 actgcaaggc tggagggaaa tccacatccc ctgatgcctg gattcgctgc tcgagaatat
     1921 tactaaccca catcggattg ggcttcaacc cgaagaaacc cggaccagaa aaggcatagg
     1981 gctggaacag gcggaaggca agcaagctga ccacagccgc caaacacaga tacgcaaaag
     2041 cctctccaag gcggcgcatg cgctcttcgg ctgggacagc cagaagcttc agcccaaata
     2101 ctaccggcag agcaaccgcc acagctccag cattcagctt ggaagccaca gcgcagccta
     2161 aggcgaaacc gaaggctatg ctgaagatga aaagagggtg cttcacaaaa cgctttgcgg
     2221 actctgttat gcccgtcatt tcccctgctg ccagcacacc ttcctctcca acctgccatt
     2281 tcgtatctcc aacccgtacc gcaaaataga tcgccagata agtgaacaga ttgagaaatg
     2341 tctccatggc gaagtaatgc gagagttgga tctgcagcac tgccactgca ctgaaagctg
     2401 ctgacagaac acccacacgc cggtcataca gtcgagtagc gatgacatat accagaaaaa
     2461 cggtgagcag atcggccata gccgacaaga agcggcctac gtcggtcatc tcctgaaatc
     2521 ctgagtgccc ataaatccat tcaacgatgt agcgcgtcag gaacatcggc aatgtcccgt
     2581 agacatagaa accgtggccg cggttattcg ggttcaaggg agaacaggat gcatcaaaat
     2641 acccgcccca ggcctgacaa tcagggaatg catcagggtg gttgcctctg gctggattga
     2701 gagcagtaga aggtggagga cctaatttct cgggatccgt acccacaggc tgaatatccg
     2761 aaccaaccca aatcaggaag cgctcgtctg ggtgtaaata ctggtattca ccccagttca
     2821 gtccgacgag acggaaatac gccccaatga ctaagacaca aaccagcagt aaatcgaaaa
     2881 ccagcgttcg gcgtgatttc tgatcaggga caatgttcat aagcatctat ggcatgatgt
     2941 gcggcacagt ttgcaataat caattcttcc ataccatcga ctcgccacac attacgcctc
     3001 acttgagcgg tggcacaaag tatatcaaga agtcatgccc ttccaccttg gaagggtaaa
     3061 cactttggat tccgtcagga tagagctggc gcaatgtgct cagcccaacc tcatcctctg
     3121 gttttagcaa gaatagcttg gcgcgtgtct ccgggagggt ctcctgcaga ttctcgggcc
     3181 agatcccata atcacgggtc ggaaatccgg cattcattgc aaccaggcga gtgtctaccc
     3241 aatgggggta agcgatcacc caggcggtat ccggagtgcc tgacccgcca gcaaaatcat
     3301 ggatgacctg cccaagctcc gacgtattcc acgaactgcg gagatatgtt tcctgatact
     3361 ggttaaacgc aatgtcgaaa ttctgaaacg atgcccagat gaccaggaaa agacccgcac
     3421 ccacagcaag tgcatttcct aacctgcctc caaactgaga cttgagcgtc tctaaaaaac
     3481 catctaaggc cagtcccaca atgaggaaaa ccggcacgat ggcaccagac aatcgattaa
     3541 gtgccggatt ctctgctggg aaagccaaag acaatattga tggcagcatc aacaaaggga
     3601 ctgctagcag ccaaaaaaga tccagccatt gtcgccgccg cagatagcga aggagaacaa
     3661 aaaccgaccc gaggtagaaa agtacagctg agaccacgtc taaagcaggt cggtgagtaa
     3721 cagagatcgc ccaaatttcc ccattatccc aagcaaacat gctcaacgcc ttccac
//
LOCUS       DBNHOH010000132        12888 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_199457, whole genome shotgun sequence.
ACCESSION   DBNHOH010000132 DBNHOH010000000
VERSION     DBNHOH010000132.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12888)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12888)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12888
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_199457"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="AC16V7_02415"
     CDS             complement(<1..431)
                     /locus_tag="AC16V7_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256000.1"
                     /translation="MKINSLFLTLLIFFASLLLVGCLGEADLPALESRPVPPLAPPAV
                     DPTVAALPRQDIFTGAQVSVSNQQAPLIPHDMIGRTDCLMCHKQGVSNAPRIPDGHRG
                     LESNTCQTCHASLASLELSGAEMYQRICTRCHGEDGEGGAGP"
     gene            complement(428..1807)
                     /locus_tag="AC16V7_02420"
     CDS             complement(428..1807)
                     /locus_tag="AC16V7_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024827.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HIR6256001.1"
                     /translation="MRKYITLIIIIFIGLLLSPAVFDVRGQESETEDTPQNDYVGSVT
                     CSRCHANTYETFKQTWHPNMLRPATDDNILGDFNTQDPDLDFTREEVDWVLGGQYKQR
                     YLTEEDGQLFVLLSEWNVETREWSAYVTGEWVPYEGQIWRKRPYAPYCAGCHTTGFNP
                     VTQTWVEPGVGCEACHGPGGDHVASTGNRTKIINPANLEFRDQVEICAQCHSRGEDPS
                     EQFPYPVGYYPGGPAKLGETFILTTDPNDFWPNGTAKRHHMQYQDWQIGEHKDSVSCI
                     FCHTSHSVGETDHQTRMVGNDRCVICHEGQTDLAAHIPFMASVADQVNCTDCHMPQIS
                     KLVPTDFEILSHTFSPPDPAVSIAYGGFEQMPNACNLCHTDKSPEWAAGVLGQEIPTA
                     DATPVPQPTPMPLPTSVFAQAGLFASEEVNPPDTQAGSGALTLWWLGLLAVILVFAFL
                     WARRRMNSE"
     gene            complement(1824..2774)
                     /locus_tag="AC16V7_02425"
     CDS             complement(1824..2774)
                     /locus_tag="AC16V7_02425"
                     /inference="COORDINATES: protein motif:HMM:NF014651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HIR6256002.1"
                     /translation="MENELLTRAEGYAYLASMFRRPETATAAEPGSTSLDIVQAYRRL
                     FLGPGRPIVSPYESVHLEGQLMGASAARVVESYAEANLRLSPDEHELPDHVSIELAFM
                     AYLAEQEQCDPDQAEVWRERQRCFLLDHLGRWLPLFCQKVEASRMHPFYSEAAQITRQ
                     LVGSDMAEFLPSVRKRRYANICLEVDGARCTLCSLCTDSCPKDALHVVSTPAQLSLLF
                     DPSHCNGCRVCLRLCPEQAINLKRNPPQDFPVLAAKQVVAVADRAVCPECHRPHVAEP
                     WLDRLAERMGGGAFALHTLKLCPFCKVAVGNSLPVSSGIP"
     gene            complement(2777..4099)
                     /gene="nrfD"
                     /locus_tag="AC16V7_02430"
     CDS             complement(2777..4099)
                     /gene="nrfD"
                     /locus_tag="AC16V7_02430"
                     /inference="COORDINATES: protein motif:HMM:NF015851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR6256003.1"
                     /translation="MNKDDKEAVLFKPVEKTGRGFYLVVAGLVLILAWAAYAYSRQIV
                     VGLDVTGLNRPVYWGLYITNFVFFIGISHAGTLVSAILRVFGAEWRRPITRAAEAITV
                     FALVLGTGNIMIDMGRPDRLLNIIRYGRFQSPLVWDVTSLTVYLTASLTYLYLPLIPD
                     LAILRDRPGLSPWRRWLYTKLSIGWQGTEEQKHRLERLIGIMAVLIIPIAVSVHTVVS
                     WVFAMTVQPMWHSTIFGPYFVIGAIFSGIATLIIVMVILRRAFNLYAYITDEHFSNLG
                     LLLMIFAFLWAYFTFSEYLTEFYGNEPAGMRVLFAKLVEEFSLMFWAMWLFNFILPFI
                     LLSNRRTRTVTGTVFTSILVNVGMWLERYNIVVPTLTRPRLPSPITSYTPTWVEWSVL
                     VGSFAVFALLYVLFSKLFPIVSIWEVREGEEIAAGGVVAGHHAEVGAD"
     gene            complement(4101..4730)
                     /locus_tag="AC16V7_02435"
     CDS             complement(4101..4730)
                     /locus_tag="AC16V7_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR6256004.1"
                     /translation="MPHWGMVIDLDRCVACQACSAACRAENNVSFAGPEETALGRAIY
                     WQEVLAEVDGEYPNVRGTFIPRPCMHCENPPCVRVCPVGATYQNEEGLVLQRYERCIG
                     CRYCMVACPYAVRYFNWRAPEWAEGMERYQNPDVPIRPKGVVEKCTFCTHRLPVPACN
                     QACPGNARFFGDLEDPDSVVSQLARSPRAFRLLEDLGTEPKVYYLREGA"
     gene            complement(4739..7120)
                     /locus_tag="AC16V7_02440"
     CDS             complement(4739..7120)
                     /locus_tag="AC16V7_02440"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-containing oxidoreductase family
                     protein"
                     /protein_id="HIR6256005.1"
                     /translation="MKSSRRDFLKLSGAIGATAIGASLVRPFFEATAQTSTLPYMQQW
                     ISTICLMCPGGCGILVRTINGRAVKIDGNPLHPVNTGKVCPKANAALQVLYDPDRLRG
                     PMVQKGERGSDGWEAISWDEATQLVAERLADIRVNPGPQAMAFMSGRVRGQMGGLIDR
                     FMAAYGSPNHIRHDSLSIDGERIGNELTFGHREQLAYDWDNCNYVLSFGRAFLEASRP
                     TVRQIRGFSAMRRDRPNRRAKFVQIDTRFSITAAKADEWVPIRTGTDGALALGIAHIL
                     IAEELYDRRFVSEHTHGFEDWTDENGVAHEGFRSMVMREYHPVQVESITGVSADTIIR
                     LAREFANAQPGVACGGRGAVMQTNGVFNWMAIQALNALVGSIEVPGGVLVQNRPPYSP
                     WPDLPADPVADAGLAMTRFDGAGTRAYPLAHSVYQDLPERILAGEPYPLGALFIYYTN
                     PLFSSPDVKRFYEAIQKVPFVVSFSPFLDDSSAYADLILPDHSFLERLQDDEIEPSLG
                     YAVLGLRQPVVAPLFDTRDTGDVLIQIARAMGGSMEAAFPWSDFSQVIQSRLSGLWAF
                     ESGSIRSTNFSHFWDELISQSVWSDAPYAFGQWGRVFNTASGKFEFFSQKLYEKLDGL
                     RGEQTWGEWLSELGIDASGDMAFMPHYESPRHIGRESEYPFFLNTYKLMTHAEGRGAN
                     TPWLQEKLGMHIKKSWDAWVEINPEVAERLGIEDDDWVWIESPIGKIKLRAVLYPGAR
                     PDTVNIPFEQGHKHYGRWAKERGENPNWVVANESDQLAGIAAWFSTRVKIYKA"
     gene            complement(7152..8204)
                     /locus_tag="AC16V7_02445"
     CDS             complement(7152..8204)
                     /locus_tag="AC16V7_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HIR6256006.1"
                     /translation="MRYYTHLVFLIIQALLVVGCAGPRSLPVGPTPIPTLIPATEPAT
                     LPGAAATPSFTILSYPARPPSTALGQPLYQTHCASCHGEDGHGVVPGARNFNDLDYIR
                     GETPAFFYSAVTEGRGEMPAFLNELTSDERWDVVFYTWRFSTNVETLALGKSIYDTNC
                     SACHGIDGVGKVLGAADFTDLRVVDARAPRDFYLTVTQGIGSMPAWQGRLSQEERWAV
                     IDYLRTFSYDATLPGEVAATPTAQVAAPEIACTTTYVSNTNPFAWDDAAASEAGELIY
                     SQSCSICHGVDGSGAMPGAPDLTTAEKQDELRSEDGEFPCIMAEGQGAMPGWKETLSA
                     DQIWQVLTYIGSLTAP"
     gene            complement(8220..8726)
                     /locus_tag="AC16V7_02450"
     CDS             complement(8220..8726)
                     /locus_tag="AC16V7_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6256007.1"
                     /translation="MTSRRQRGLIFIAIILAPFIIGLLFTYEIIKIPFLTDMADQPSI
                     GYQEGPRILPPEGAVPVQGPSLIIQEFPVNPVESDEVSLQRGAILYDIHCQLCHGSQG
                     HGDGPISHYFDRTPQNLTAAQITGEFDGSVYLTILQGFGQMPSLAENLTPRERWDVIN
                     YVRTLPPQ"
     gene            complement(8723..9247)
                     /locus_tag="AC16V7_02455"
     CDS             complement(8723..9247)
                     /locus_tag="AC16V7_02455"
                     /inference="COORDINATES: protein motif:HMM:NF023249.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:electron acceptor oxidoreductase subunit
                     ActD"
                     /protein_id="HIR6256008.1"
                     /translation="MNETQALLALFYESTDTAESIDELHSLGIPDDKVVVITGVPYPE
                     RALGRHSEWLTLPYIVIAGAIGGMLFGFFLAVITPHLYRLDVGGHPPVGFPPAAVIVF
                     VFTMMATIVSTFLGVLWEMDFPHFGPVPYHKLVTDGHLAVLVEYPTDLERQVHQVFES
                     HHAHYVGEPERMTL"
     gene            complement(9293..10711)
                     /gene="nrfD"
                     /locus_tag="AC16V7_02460"
     CDS             complement(9293..10711)
                     /gene="nrfD"
                     /locus_tag="AC16V7_02460"
                     /inference="COORDINATES: protein motif:HMM:NF015851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR6256009.1"
                     /translation="MSEPNNSTHEATQDSFYDERLLLAERTDEEINQAVLQPMFQSGK
                     GFWVLVTVLSLIVVWGMYAWGYLIYWGIGVSGKNRPVYWTLFITTFVFWVGISHAGTM
                     ISAVLRLLKADWRRPITRGAEAMTAFALMMAGLYPLIHLGRVWKFYWMIPYPNNRLMW
                     PNFQSPLMWDMVAIFTYLIGSSLYLYLALIPDMAMARDHTDSWRYKLYRVMALGWRGT
                     EAEWHKLHRALSIFSVAIIPIFLSVHSIVSWDFAVTIQPRWHSTIFAPYFVIGAVYSG
                     LSALASILVIVRRGMRLQEFLRAEHFDSLGKLVMVAGIAWFYFWFAGFIVEWYGNEPV
                     TRELIYQEVAGHLAPFFYLQMGANLIPVVLLVFKRIRTTPVLLLLTTLLVNVGMFTER
                     ALIVIGSLQRNEIPFNWGVYRPTWVEISIVAMTFAGFALLYTLFSRIIPFVPVWEIKE
                     GRLRYTVRRIGRLLVPTAAEIE"
     gene            complement(10704..11429)
                     /locus_tag="AC16V7_02465"
     CDS             complement(10704..11429)
                     /locus_tag="AC16V7_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR6256010.1"
                     /translation="MAKVANLSAEGNHRWVLVVDLDRCTGCQACVVACHAENNVPVVG
                     EKQAAYGRWMHWIVIHRYWEGEYPNIKARFMPVMCQQCTEAPCEPVCPVFATYHNAEG
                     LNAQIYNRCIGTRFCGNACPYKTRTYNWFEPEFPEPLNEQLNPDVSVRTRGIMEKCTF
                     CIQRIRRTEIDAQAEGRAPADGEIQPACVQTCPTNALVFGDLGDPDSLASQMARSRRS
                     FRLLPELGTDPGVIYLKGGEIIV"
     gene            complement(11401..>12888)
                     /locus_tag="AC16V7_02470"
     CDS             complement(11401..>12888)
                     /locus_tag="AC16V7_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HIR6256011.1"
                     /translation="GLLVQFEPRLSSTAASADKWIPLKPGAEGFVALAIGRIIVEERL
                     GHVGSHRPHAVLYQNVDVRQMAAASEVPVETLRQLAQAFADADRPVAIPGGYLAGQHN
                     GISSMLAVQALNLVVAQVGREGGVFLSNPGPTVALRQKPAAHTFNEMLDLIARMKAGE
                     VDLLIVHSTNPMFELPAAAGFAEAISRVPFVVSFSQFVDETTVWANLVLPDHTPLEAW
                     GYQVPEPGADRPVISGQQPVVRPLYDTRATADVLLTLAARLGGKAAEALPWEDEVAYL
                     EEITGELYGSSLGAYDARTSDGFWSLFRQYGGWWSEKEILREPELTEVVQSPLPISEP
                     RFDGDLQDYPFHLYPYSSIALSDGRGANLPWLQETPDPMTSVQWATWVELNPETAGSL
                     GVSDNDIVLVESPYGKLEAPVVVYPGIRPDVVAIPIGQGHKDYGRFAQVASGSNPLAL
                     VAPVTDNEIGVLAWGTTRVRLKPTGRKHILARVESLDGKGRESLG"
BASE COUNT         3202 a         3578 c         3394 g         2714 t
ORIGIN      
        1 ggcccagccc ccccctcgcc gtcctcacca tggcagcgag tgcaaattcg ctggtacatc
       61 tccgcaccgc tcagctcgag gcttgctaac gaggcgtggc aggtctggca agtattgctc
      121 tctaaacccc gatggccatc tgggatgcgc ggggcattac tcacgccttg tttgtgacac
      181 atgagacagt cggtccgacc aatcatgtcg tgcggtatca acggggcttg ctggttagaa
      241 accgacacct gtgccccagt gaagatgtct tgacggggca aagcagccac ggttggatcc
      301 accgccgggg gtgcgagggg cggtactggc ctgctttcca aggctggcag atctgcttct
      361 cccagacagc caacaagcag taacgaagca aagaaaatga ggagggttag gaacaaagag
      421 tttattttca ttcagagttc atcctgcgac gtgcccataa gaaagcaaat actaagatca
      481 cggccaggag tcccaaccac cagagcgtga gagctccaga tccggcttgt gtatccgggg
      541 ggttgacctc ttcactcgca aacaggcctg cctgggcaaa aacgcttgtt ggcaaaggca
      601 ttggtgttgg ttggggcacc ggggttgcat ccgctgttgg gatttcttgt ccgagcacac
      661 cagcagccca ttcgggtgat ttgtcggtat ggcacaaatt acaggcatta ggcatctgct
      721 caaaaccacc ataggcaatg ctgactgctg gatcgggcgg gctaaacgtg tgactcaaga
      781 tctcaaaatc ggtggggacg agcttggaaa tttggggcat atggcagtcg gtgcagttga
      841 cctgatctgc cacgctagcc ataaaaggaa tatgtgcagc caggtccgtc tgaccttcgt
      901 ggcaaatcac acagcggtca ttgcccacca tgcgggtctg gtgatcggtc tcgccaacgc
      961 tgtgagaggt gtggcagaag atgcagctca cactgtcttt atgctcaccg atctgccagt
     1021 cctgatattg catgtggtgg cgtttcgctg tcccattggg ccagaaatca ttcgggtccg
     1081 tggtaaggat aaaggtctct cccaatttgg ctggaccacc tgggtaataa cccactggat
     1141 atgggaattg ctccgaaggg tcttcaccac ggctgtggca ttgggcgcag atttcgacct
     1201 gatctcgaaa ttccagattg gcagggttga tgatttttgt ccggttgcca gtggaggcca
     1261 cgtgatcccc cccggggccg tgacatgcct cgcaccccac cccgggctcc acccaggtct
     1321 gggtcaccgg attaaagccc gtggtgtggc aacctgcaca atatggcgca tagggacgtt
     1381 tgcgccaaat ttggccctca tagggcaccc actccccagt cacataggca gaccactctc
     1441 tggtctcgac attccactct gacagaagga caaatagctg accatcttct tccgtgaggt
     1501 agcgttgttt atactgccca cccagcaccc aatccacttc ttcgcgcgtg aaatccaggt
     1561 cgggatcctg ggtgttgaaa tcacctaata tattgtcatc cgtggcaggt cgcaacatat
     1621 tcggatgcca ggtctgtttg aacgtctcat aggtgtttgc atggcagcga gagcaagtga
     1681 ccgaaccaac ataatcgttt tgcggggtat cttcggtctc tgattcttgt cccctgacat
     1741 caaaaacagc tggagagagc aacaatccga tgaagatgat gatgatgagc gtgatatatt
     1801 ttctcaacac ttgaaatcga ctcctatggg atgccactcg agacaggcag gctgtttcct
     1861 acagccactt tacaaaaagg gcacaatttg agcgtatgca aagcaaatgc tccaccaccc
     1921 atacgctcgg ctaaacgatc cagccacggc tcagcaacgt gcgggcggtg gcactcagga
     1981 cagacagcgc gatcggccac cgcgacaacc tgcttggcag ccaacacagg aaaatcctgc
     2041 gggggattgc gctttaggtt gatcgcttgt tctggacaca gccgcaggca tacccggcaa
     2101 ccattgcaat gggatgggtc aaaaaggagg cttaactgtg ctggcgtgct gacaacgtgc
     2161 agcgcatctt tcgggcagct atcagtgcaa agcgagcaca gagtgcagcg ggcgccatcc
     2221 acttcgaggc aaatattggc gtaacgcctt tttcgtacag aaggcaggaa ctctgccatg
     2281 tcagatccaa caagctgccg cgtgatctgc gctgcctcac tatagaaagg atgcatccgg
     2341 ctggcttcga ccttctggca aaacaggggc aaccagcgac ccaagtggtc tagaagaaag
     2401 cagcgctggc gctcccgcca aacttccgcc tgatcagggt cacattgctc ctgttctgca
     2461 aggtaagcca taaaggccag ttcgatggag acatgatctg gcaactcgtg ttcatcagga
     2521 gacaatcgca ggtttgcttc ggcatacgac tcgaccacac gagcagcgct ggcccccatt
     2581 aactgccctt ccaggtggac tgactcgtaa gggcttacga tgggacgacc tggacccagg
     2641 aagaggcgcc ggtaggcctg gacgatatcc aaactggtag aacccggttc agccgctgtg
     2701 gcggtctcag gacgccggaa catcgatgcc agataggcgt aaccctctgc gcgggtgagc
     2761 agctcgtttt ccatgatcag tctgcaccca cctcggcgtg atgacccgcc acaacccctc
     2821 cagcagcgat ctcttcccct tcacgcacct cccaaatcga aacgataggg aacagcttag
     2881 aaaatagcac atacaacaag gcaaacacgg caaaactgcc aaccagcacc gaccactcca
     2941 cccaggttgg ggtgtatgaa gtaatggggg atggcaggcg tggacgcgtg agcgtaggaa
     3001 cgacgatgtt gtagcgctcc agccacatgc cgacattaac caggatcgat gtaaatacag
     3061 tgcctgtcac ggtacgcgtg cggcggttac tcaacaggat aaatggcaag atgaagttga
     3121 acagccacat ggcccagaac attaaggaga attcttcaac cagcttggcg aacaagaccc
     3181 gcataccagc tggttcgtta ccatagaact cggtcaggta ctccgagaat gtaaagtagg
     3241 cccacaggaa agcgaatatc atcagcaaca atcccaggtt gctgaaatgc tcatcggtga
     3301 tataggcata caaattaaat gcgcgccgca ggatcaccat cacgatgatt agggtggcaa
     3361 tgccggagaa gatggcgcca atcacaaaat acggtccaaa aatagtgctg tgccacatgg
     3421 gctgaaccgt catggcaaac acccacgaaa ccacggtgtg caccgacaca gcaatcggta
     3481 tgatcaacac tgccataata ccgatgagcc tttccagacg atgtttctgt tcttcggttc
     3541 cctgccagcc tatagagagt ttggtgtaaa gccagcgtcg ccagggtgac aacccggggc
     3601 gatcgcgcag gatggccaga tcagggatga gaggcaggta gagataggtg aggctggcag
     3661 tcaggtaaac tgtcaaggag gtcacatccc acaccagagg cgattgaaag cgcccatagc
     3721 gaatgatgtt taacaaacgg tcggggcggc ccatgtcaat catgatgttg ccggttccaa
     3781 ggaccaaggc gaacaccgtg atggcttcgg cagccctggt aattgggcgg cgccattcag
     3841 ctccaaacac gcgcaggata gccgaaacca acgtcccagc atggctgatg ccgatgaaga
     3901 agacgaagtt ggtgatgtac aagccccaat aaaccggcct gttcagcccc gtgacatcca
     3961 gaccaaccac aatctgtctc gagtaggcat atgcagccca ggcaaggatc aataccagac
     4021 cagcgacgac cagataaaac ccgcgtccgg tcttttctac cggtttgaac aagacagctt
     4081 ctttatcgtc tttgttcaca ttacgctcct tctcgcaagt aataaacttt tggctctgta
     4141 cccaaatcct ccaacaacct gaaggcgcgc gggctgcgcg ctaattggga taccacgcta
     4201 tcagggtctt ccaaatcgcc aaaaaagcgc gcgtttcccg gacaggcctg gttacaagct
     4261 ggaactggca agcggtgagt acagaaggtg catttttcga caacgccctt ggggcggata
     4321 gggacatctg gattttggta acgttccatc ccctcagccc attcgggggc acgccagtta
     4381 aagtagcgca ccgcataagg acaggccacc atacagtagc ggcatccaat gcaacgttcg
     4441 tagcgctgca ataccaatcc ctcttcattt tggtaggtag cccctaccgg gcacacgcga
     4501 acacaaggcg ggttttcaca gtgcatacaa gggcgaggga taaacgttcc gcgcacattg
     4561 ggatattcgc catccacctc agcaagcact tcttgccaat aaattgcccg cccgagagct
     4621 gtttcttcag gtccggcgaa agaaacattg ttctctgccc ggcaggcagc cgagcatgcc
     4681 tggcaggcca cacaccgatc caagtcaatc accattcccc aatgaggcat atttactcct
     4741 aagctttgta gatcttcacg cgcgtcgaaa accaggccgc aatgcccgcc agttggtcgc
     4801 tttcattcgc aaccacccaa tttgggtttt ctccgcgctc cttggcccaa cgaccgtagt
     4861 gtttatgacc ctgttcgaag ggtatgttta ccgtatctgg acgggcaccc ggatatagga
     4921 cagccctaag cttgatttta ccgatgggag actcgatcca tacccaatca tcatcctcta
     4981 tgcccagcct ctccgcgacc tctggattaa tctcaaccca cgcgtcccag cttttcttga
     5041 tgtgcatgcc gagtttctct tgtagccagg gggtatttgc tccccgcccc tcggcatgtg
     5101 tcatcagctt ataggtatta aggaagaagg gatactcact ctctctcccg atgtgcctgg
     5161 gggactcata gtggggcatg aaggccatgt ctccgctggc atcgattcct aactcactta
     5221 accactcgcc ccaggtttgc tctccacgca acccatccag tttttcgtat aatttctgcg
     5281 aaaagaattc aaatttaccg gaagcggtat tgaagacgcg accccactgc ccgaaagcgt
     5341 aaggcgcatc cgaccagaca ctctggctga tcagctcatc ccagaagtgg gagaaattcg
     5401 tcgaccggat cgacccgctt tcgaaggccc acaagccgct gagacggctt tggatgacct
     5461 gagaaaaatc agaccaggga aaagccgcct ccatagaccc gcccatggcg cgtgcaattt
     5521 gtatgagcac atcgccggta tcgcgggtat caaacaatgg tgcaaccacg ggctgtcgca
     5581 aacccaaaac agcataccct aagctaggct cgatctcgtc atcctgcagc cgttccaaga
     5641 aggaatgatc aggtaagatc aagtccgcat aagcgctgct atcgtcgagg aaaggcgaaa
     5701 aggaaacaac aaacggcacc ttctgaatag cttcgtaaaa tcgcttgaca tccggcgagg
     5761 agaaaagcgg gttggtgtag taaataaaaa gcgcgccaag cggatagggc tcgccagcca
     5821 ggatgcgttc aggcaagtct tgataaacgc tgtgcgccag ggggtatgca cgcgtaccgg
     5881 caccatcaaa gcgcgtcatg gcaagccctg catcagcaac tggatctgcg ggcaggtcag
     5941 gccaaggtga ataaggcgga cgattttgca ccagcactcc cccaggaact tcgatgctgc
     6001 cgaccagggc attcagcgcc tggatcgcca tccagttgaa aacaccattg gtctgcatca
     6061 cagcaccacg ccctccgcac gccacgccag gctgcgcgtt ggcaaattcg cgtgctaagc
     6121 ggatgatcgt atcggccgaa acacccgtaa tcgattccac ctgtacggga tggtactctc
     6181 gcataaccat ggagcggaag ccctcatgag cgacgccatt ttcatcggtc caatcttcga
     6241 agccgtgtgt gtgctccgaa acaaaacgcc tgtcatagag ctcctctgcg atgaggatgt
     6301 gggcaattcc aagtgccagt gcgccatcag taccagtgcg aattggcacc cactcgtctg
     6361 ccttggcagc tgtaatcgaa aagcgcgtgt cgatctgcac aaatttagcg cgtcgattcg
     6421 gtcgatcgcg ccgcatcgcg ctgaatccac gtatctggcg cacggttggc cgggaggctt
     6481 ccagaaaagc gcgcccgaag ctgagcacat agttgcagtt gtcccagtca taagccaatt
     6541 gctcgcggtg accaaaagtt agctcattgc caatgcgctc cccatcgata ctgaggctat
     6601 catggcggat gtgattgggc gagccgtagg cagccatgaa gcggtcaatc aggcctccca
     6661 tctggccccg cacgcggcca gacatgaacg ccatggcttg aggtccagga ttcacgcgga
     6721 tatctgccag gcgctcggcg accaattggg tcgcttcgtc ccaactgatt gcctcccaac
     6781 cgtcacttcc gcgctctccc ttttgcacca tcgggcccct taaccggtct ggatcgtaaa
     6841 gcacctgcaa agcggcgttt gctttggggc atacttttcc tgtgttgaca ggatgaagtg
     6901 gattgccatc aatcttgacc gccctgccat tgattgtccg gaccaaaatt ccgcacccac
     6961 ctgggcacat taagcagatc gttgatatcc actgctgcat gtaagggagg gtgctggttt
     7021 gggcagtggc ttcgaagaac gggcgcacca ggctcgctcc tatggcggtt gcgccgatag
     7081 cgcctgaaag tttcagaaag tcacgacgtg aggatttcat tttgtaactc atatggtcga
     7141 tccaaaggat gtcatggggc tgtgagtgag ccaatatagg taagcacctg ccagatttgg
     7201 tcggcgctta gggtctcctt ccaaccgggc atcgcgccct gaccttcagc cataatgcac
     7261 ggaaattccc catcttctga gcgtaattcg tcttgctttt cagctgtagt taagtcaggt
     7321 gcacctggca tggcacccga accgtctaca ccgtgacaaa tactacagct ctggctgtag
     7381 attaactcac cggcttcact tgcggctgca tcgtcccagg cgaagggatt tgtattggat
     7441 acatacgtgg tagtgcaggc gatctcaggg gcggctactt gtgctgtagg cgttgcggcg
     7501 acttctcccg gcagagtagc gtcatatgag aatgttcgca ggtagtcgat cactgcccag
     7561 cgttcttctt gagagagacg cccttgccag gcaggcatgg accctatgcc ctgtgtaacg
     7621 gtcaagtaga aatcgcgggg agcccgagcg tccaccactc gcaggtcagt gaagtcagcc
     7681 gcgcccaata ctttacccac accgtcgatg ccatggcagg cactgcagtt ggtatcatag
     7741 atactcttac caagagcgag ggtctcaacg ttggtggaaa aacgccaggt gtagaaaacc
     7801 acatcccacc gttcatcaga tgtcagctca ttcagaaatg ctggcatttc gccgcgtccc
     7861 tctgtgactg ctgagtagaa gaaggcgggg gtctcgccac gaatgtaatc cagatcattg
     7921 aagttacgtg ccccaggcac aacgccatgc ccgtcttcgc catggcaact tgcacaatga
     7981 gtctggtaga gcggctggcc tagagcagtt gagggtggcc gagccggata actaaggatg
     8041 gtgaaggatg gcgtggcagc ggcgcctggg agggtagctg gttcggttgc cgggataagc
     8101 gtaggtatag gagtgggacc gacaggtaag ctgcgcggac cggcacaacc taccacgagc
     8161 aacgcctgta tgataagaaa taccaggtgt gtgtagtatc tcattaaggt gacctctcat
     8221 tattgcggcg gcaaagttcg gacataatta ataacatccc agcgctcgcg tggtgtcagg
     8281 ttttcagcca gcgaaggcat ttggccgaat ccctgcagaa tggtcaagta gaccgagccg
     8341 tcaaactcgc cagtaatctg tgcggcggtt aagttctgcg gcgttcggtc aaaatagtgg
     8401 gagattggtc catcgccgtg cccctgtgaa ccatgacaga gttggcagtg gatatcatat
     8461 aggatagccc cgcgctggag agaaacctcg tcggattcca ctgggtttac ggggaactcc
     8521 tggataatta aggaaggccc ctgcacaggc accgccccct caggaggcag tatacgcggt
     8581 ccttcctggt aacctattga aggttgatca gccatatcgg tgagaaacgg gatcttgata
     8641 atctcgtatg tgaataacaa gccgatgatg aatggcgcaa gaattatagc gataaaaatc
     8701 aagccccgtt gccggcgcga ggtcatagcg tcatcctttc tggctcgcca acatagtgtg
     8761 catgatggga ttcaaatacc tggtgtacct gacgctccag atcagtcgga tattccacca
     8821 aaacagccag gtgaccatct gtaacaagtt tatgatacgg aaccggccca aaatgtggga
     8881 aatccatctc ccaaagcaca cccagaaatg ttgagacgat ggtagccatc atggtaaaaa
     8941 caaagacgat cactgcagcc ggcgggaagc caactggggg atggccacct acatccaggc
     9001 ggtaaaggtg gggcgtgata accgccagaa agaagccaaa cagcattccc cctatcgctc
     9061 cagcaatcac gatatagggg agagtcagcc actcactatg ccgtcctaag gcgcgttcag
     9121 gatatggcac gccagtgatc accacgactt tgtcgtcggg gatacctagg ctgtgcaact
     9181 catcgattga ttcagccgta tcagtgctct cgtaaaagag ggctaataaa gcctgtgttt
     9241 cattcatatg tcgtcaacca tctccaccag ctatcatgtg attggattct tctcattcaa
     9301 tctccgctgc cgtcggcacc aacaaccgcc cgattcgcct tacggtgtaa cgcaagcgtc
     9361 cttccttgat ctcccacact ggaacgaatg ggatgatacg ggaaaacagc gtgtacagca
     9421 gcgcaaagcc ggcaaaggtc atggcaacga tgctaatctc aacccaagtc ggacgataga
     9481 caccccaatt gaagggtatc tcgttacgtt gcagacttcc aattacgatg agcgcccgct
     9541 cggtaaacat accaacgttg accagcaagg tggttaacag cagcaacacc ggggtagtac
     9601 ggatccgttt aaaaaccaaa aggacgactg gaatcagatt ggcacccatt tgcaaataga
     9661 aaaacggcgc tagatgccca gccacttcct ggtagatcaa ttcgcgggta accggctcgt
     9721 tgccatacca ctcaacgata aacccagcaa accagaaata aaaccaggct attcctgcca
     9781 ccatgaccag cttgcccaat gaatcaaaat gttcagcgcg caggaattcc tgcagtcgca
     9841 ttccacgccg cacgatcacc aggatgctgg ctagggcaga gagaccggaa taaaccgcac
     9901 cgatgacgaa gtagggtgca aagatggtac tgtgccagcg cggctggatg gttactgcaa
     9961 aatcccacga cacaatcgag tgaacggaga ggaagattgg gataatggcg accgaaaaga
    10021 tactcaatgc gcgatgcagc ttgtgccatt cggcttccgt gccacgccaa ccgagcgcca
    10081 tcacacggta aagtttatag cgccagctat cggtgtgatc gcgcgccata gccatatctg
    10141 ggatcagcgc cagatatagg tacagggatg agccaattaa ataggtaaag atggcgacca
    10201 tatcccacat caacggggat tgaaagtttg gccacatcaa gcgattattg gggtaaggga
    10261 tcatccagta gaatttccat acccggccca ggtgaataag gggatataac ccagccatca
    10321 ttaacgcaaa agcggtcata gcttcggcac cccgggtaat tggccgccgc caatcggctt
    10381 ttagcaagcg taatacagcc gaaatcatcg tgccagcatg gctgatgccg acccaaaaga
    10441 cgaatgtggt gataaagaga gtccagtaga ctggtcggtt tttaccagat acgccaatgc
    10501 cccaatagat aagataaccc catgcataca tgccccagac aacgatcaaa cttaagactg
    10561 tcacgagcac ccagaaaccc ttgccacttt gaaacattgg ctggagtacg gcctggttaa
    10621 tctcttcatc agttctttcg gccaggagca agcgctcgtc gtaaaacgag tcttgtgttg
    10681 cttcgtgtgt cgaattatta ggctcagaca attatctcgc ctcctttgag gtagataact
    10741 cccggatcgg tacccaattc tggcaaaaga cgaaaagagc ggcggctgcg tgccatttgg
    10801 ctggctaggc tgtctggatc gcccagatcg ccaaaaacca gcgcattggt gggacaagtc
    10861 tgtacgcaag caggttgaat ttcaccgtcc gcaggtgcgc gtccctccgc ttgcgcatcg
    10921 atctctgttc ggcggatgcg ctggatacaa aaggtgcatt tctccatgat gccacgcgtc
    10981 cgaacggaca catccgggtt gagttgctcg ttcagcggtt cggggaactc cggttcaaac
    11041 cagttataag ttcgagtttt atacggacaa gcattgccac agaaccgcgt gccaatacag
    11101 cggttgtaga tttgcgcgtt caagccctca gcgttgtgat aggtcgcaaa cactgggcag
    11161 accggctcac agggggcctc tgtgcactgc tggcacatca caggcatgaa acgtgccttt
    11221 atattcgggt actcaccctc ccagtagcgg tgaatgacga tccagtgcat ccaacgccca
    11281 taggctgctt gcttctcacc gaccaccggt acattgttct ctgcatggca agcaacgacg
    11341 cacgcttggc agccagtaca gcgatccaga tcgaccacca gcacccatcg atgattaccc
    11401 tcagccgaga gattcgcgac ctttgccatc taaactctcc actctcgcta aaatatgctt
    11461 gcgtccggtt ggcttcaggc gtacgcgtgt ggttccccag gccaatacgc ctatttcatt
    11521 gtctgtgacg ggagctacca aggccaacgg gttgctacca ctggccacct gagcaaaacg
    11581 tccataatct ttgtgtccct gaccgatcgg gatggcaacc acgtctggac gaatccccgg
    11641 atagacaaca accggtgcct ccaacttacc gtacggcgat tcaactagca cgatatcgtt
    11701 atcactcaca cccagcgagc cggcagtttc aggatttaat tccacccagg ttgcccattg
    11761 cactgacgtc atcgggtcgg gcgtttcctg aagccatggc aaatttgcgc cacgcccatc
    11821 cgaaagagct attgaagaat agggatacag gtggaaagga taatcttgca ggtcaccgtc
    11881 aaatcgcggc tcggatatag gtagagggct ttgcactacc tctgttagtt cgggctcacg
    11941 gaggatctcc ttttctgacc accagccccc atattggcgg aagagtgacc agaagccatc
    12001 cgaggttcga gcatcgtatg cgccgaggct agaaccgtac aattcacccg tgatttcttc
    12061 caaataagct acctcatctt cccaaggaag tgcctctgct gccttaccac cgaggcgtgc
    12121 agccagggta agcagaacat ccgcggtggc gcgagtatca taaagtggac gcaccaccgg
    12181 ttgctgcccg ctaataacag gacggtctgc gcctggctca ggcacctgat agccccaggc
    12241 ctccaaggga gtgtggtcag gcaagaccag gttcgcccat actgttgttt cgtcaacaaa
    12301 ctggctgaag gaaacaacaa aaggcacacg agagatagct tctgcgaaac cggctgcagc
    12361 tggtagctca aacataggat tcgtgctatg gacaatcaac aggtcaactt ccccggcttt
    12421 cattcgggcg atcagatcca gcatctcgtt gaacgtatga gccgcaggtt tttgtcgcaa
    12481 agccactgtt gggcccgggt ttgaaagaaa tactccaccc tcccgaccaa cttgtgctac
    12541 tactagatta agcgcctgta cggccagcat gctggaaata ccgttgtgct gtccagccaa
    12601 gtaaccacca gggatagcca caggtcgatc ggcatcggca aaagcctgtg caagctgacg
    12661 cagtgtttca accggcactt cactagcagc ggccatctgg cgcacatcca cattctggta
    12721 caacacggca tgaggtcgat gactgccaac atgaccgagt cgttcctcaa cgatgatgcg
    12781 cccgatggca agggcgacaa acccctcagc tccaggcttc aacggaatcc atttatcagc
    12841 tgacgctgcc gtcgacgaaa ggcgcggctc aaactgcacg agcaatcc
//
LOCUS       DBNHOH010000133         1863 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1289311, whole genome shotgun sequence.
ACCESSION   DBNHOH010000133 DBNHOH010000000
VERSION     DBNHOH010000133.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1289311"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..417)
                     /locus_tag="AC16V7_02475"
     CDS             complement(166..417)
                     /locus_tag="AC16V7_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256012.1"
                     /translation="MTTILFDPIRYHEARYQQLRDEAQHSRLVKEALQARQPKARGAS
                     NFLAVLGRKLADLGAALEKRYDTPPTAVVQLKTQSNSDC"
     gene            551..1174
                     /locus_tag="AC16V7_02480"
     CDS             551..1174
                     /locus_tag="AC16V7_02480"
                     /inference="COORDINATES: protein motif:HMM:NF018984.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HIR6256013.1"
                     /translation="MSECSFDFDSGNLSLDFANTKEWHASENPKERLTNYADLIAWGK
                     EAGLISPDEARRLRHLAVDQPGKAATSFEFAIQVREAIYAIFSMHYDGEPIPVNEMAL
                     LNSVVREAMSHLQLVPRGGEITWDWVPEIDGANLILWPVARSAAELLTSDKLDRIREC
                     EDERGCGYLFIDQSKNRSRRWCSMDSCGNRAKARRHYAKVQEEFSES"
     gene            1264..>1863
                     /locus_tag="AC16V7_02485"
     CDS             1264..>1863
                     /locus_tag="AC16V7_02485"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase family protein"
                     /protein_id="HIR6256014.1"
                     /translation="MAPFRYQDQANWFNYVIRLDSGSVVDLHIQDAQDGVQLRVDNSL
                     SETECDSITRAASWMLGLDQDFSTFYKIAQTEPKLEHLVTAARGRILRSPTLFEDIIK
                     TILTTNTLWAGTIRMAETLVAIYGDPIPDDPSRRAFPTPERLADLDESALRSSARLGY
                     RAPYVLELSRQVASGNLDLESLKNSDLPTEQLRARLMDIK"
BASE COUNT          459 a          478 c          464 g          462 t
ORIGIN      
        1 atataaacga ccatacctgc aattagaaaa cgtgtggctg ccatcagaaa tggcggcatg
       61 ctctccacgg caaagcgtat cgccagatag gtcgatcccc aggcaatata tactgctatc
      121 agtgctagcc agattcgcga tttcatctat cccaactttc gtttatcaac aatcagagtt
      181 ggactgagtc tttagttgca ccacagctgt aggcggcgta tcgtagcgtt tttccaaagc
      241 tgcgccgaga tctgccagct ttctacctaa tactgccagg aaattcgaag ctcctcgggc
      301 tttaggttgc ctcgcctgca acgcttcttt caccaggcga gagtgttgcg cttcatcgcg
      361 cagctgctga tacctggctt cgtgatatct gatggggtcg aacaatattg tggtcatttg
      421 atctctcctt caattgcatc acttctcttg acaaccactt aataacctgc tatcatgtgt
      481 ttactggtta tagtatagca aactatttat aacctgtcaa gagtattttg atggttataa
      541 cggagatttt atgagcgaat gttcctttga tttcgactcc ggaaacctga gtctggattt
      601 cgccaatact aaagaatggc acgccagcga gaatcccaag gagagattga caaattatgc
      661 tgatctaatt gcctggggaa aagaagctgg tctgatctcg ccagatgagg ccaggcgatt
      721 gcgccattta gccgttgatc aaccgggcaa agccgctacg tcatttgagt tcgccattca
      781 agtgagggaa gcgatctatg ccattttttc tatgcactac gatggcgagc ccatccctgt
      841 gaacgaaatg gcgctgctca actctgtggt tcgcgaagca atgtcgcact tgcagctggt
      901 gcctcgtgga ggtgagatta cttgggactg ggtaccagag atagatgggg ccaatctcat
      961 cctgtggccg gtagcgcgct ccgctgctga gctgctgaca tcggataagt tagatcgcat
     1021 tagggaatgc gaggacgagc gggggtgtgg ctatttgttc atcgaccaga gcaagaaccg
     1081 cagccgccgc tggtgttcaa tggattcctg cgggaaccgc gccaaggcgc gccggcatta
     1141 cgccaaagtt caagaagaat tttccgaaag ctaatcgcag ataacaaata tatggagctg
     1201 tacctccccg cacagcaacc cttttcatta caagcggtga taagatcgca tggctggata
     1261 aggttggcac ccttccgtta tcaagatcaa gccaactggt ttaactacgt tatccggctt
     1321 gatagcggct cggttgtcga cctacacatt caagatgcgc aagacggtgt gcagcttagg
     1381 gtggataatt cattatcgga aacagaatgc gatagtatca cacgggcagc gagctggatg
     1441 ctcggcttgg atcaagattt ttccaccttc tataaaatag ctcaaactga accaaagcta
     1501 gaacacttgg taacggcagc acgtggtcgc attctgcgca gccccaccct cttcgaagac
     1561 atcatcaaaa ccatcttgac aaccaacacg ctttgggcgg gtaccattcg catggcggaa
     1621 acactggtcg ccatctatgg tgatccaatc cccgatgatc catctcgccg agccttcccc
     1681 acacctgaac gcctggccga tcttgacgag agtgctttgc gctcctcagc gcgcctgggt
     1741 taccgcgccc cctatgtgct ggagttatcc aggcaggtcg cctcggggaa tctcgatctc
     1801 gagagtctca aaaatagcga tcttcccacc gagcagttgc gggcgagact gatggacatc
     1861 aaa
//
LOCUS       DBNHOH010000134         2993 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_969175, whole genome shotgun sequence.
ACCESSION   DBNHOH010000134 DBNHOH010000000
VERSION     DBNHOH010000134.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_969175"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="AC16V7_02490"
     CDS             complement(<1..383)
                     /locus_tag="AC16V7_02490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR6256015.1"
                     /translation="MKIVVLIKMVPDLVEELSISPSGTELDTTWLRLKINEFDDHAIE
                     QAILLKEQYGGEVTVLAPEMEGVDDALFTAAAKGADRLIQLSGKFDSSWNNHALARAF
                     LPLAQELQPDLVLTGVQAHNDLDGSL"
     gene            complement(406..1611)
                     /locus_tag="AC16V7_02495"
     CDS             complement(406..1611)
                     /locus_tag="AC16V7_02495"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR6256016.1"
                     /translation="MATKSITQRPSFNDMFYSSLFKLPEGQRMQACLQCGTCSGVCPF
                     GYLMEYPPGKMIAALRAGLFERVINYESLWMCVSCYACTQVCPAKIPLTPGLLTRAKE
                     EMVLAGNVPSELQDALENSQRYGNPLGESPRKRAMWARGIDPPARIMSQVGGPVDVLW
                     FVGDYASFHPRVQMVSQAVAQVFNVIGVDFAILGPEEFSDGDSQRLAGERGLFEMLAE
                     KNGQVFNKYTFSQIVTTDPHAYNALKNEYPKLGIVYPVKHYTQFLVDRLDQLKPHLRE
                     EIQARVTYHDPCYLGRANGEYDAPRRLLEAIPGVKLVEMTHHMETGLCCGGGGGGMWL
                     DGFTWEKAHVRLSEWRVREAVQAEAEILAVACPYEPPRFEDAIKMIEKAKRLVVRDVA
                     ELLAEAMGE"
     gene            2106..>2993
                     /locus_tag="AC16V7_02500"
     CDS             2106..>2993
                     /locus_tag="AC16V7_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256017.1"
                     /translation="MNSVRPRWSFYTYLLVLSIFGIALVFINTSTYGPGISSDGLHYL
                     STADSLINGRGFTNFHGGVYILWPPLYPLILAGLSILTGLDTLIIGWILNAVAFGVTI
                     FLSGVLLKTCFPDQPAWQALGAALVLFFMSYVVLASNIGSDQLFVVLVLGSFLACHRY
                     MTTSNRGALWVLLILVALAPLLRFLGVCVVAVGALAVFLKNIKQPVNSTGKSIIFGLV
                     ASLPTLVWVIGRNYRLYGSLTGPRQLHNALIIENIAYCTRQILRWFVPLSVIDRLPLW
                     VLPALVLVVLLLFARKRHWA"
BASE COUNT          683 a          820 c          695 g          795 t
ORIGIN      
        1 agcgacccat ccagatcgtt gtgagcttgc acaccggtta ataccaggtc aggctgtagc
       61 tcttgcgcta atggtagaaa agcgcgcgct agagcgtggt tattccagct actatcgaac
      121 ttcccactga gctggatcag ccggtcggcc cctttggcag cagctgtgaa tagcgcatca
      181 tcaacccctt ccatttcagg agccagtacc gttacttcac caccatactg ttctttgagc
      241 aagatagctt gctcaatggc gtgatcatcg aattcattaa tcttcaggcg caaccaggtg
      301 gtatcaagct ctgttccact aggggagatg ctaagttctt ccaccaggtc tggcaccatt
      361 ttgatgagga ctacaatctt catcctttgc tcctctctca agatctcatt cacccatcgc
      421 ttcggctagc aactcagcga catccctgac aaccagccgt ttggccttct caatcatctt
      481 gatggcatct tcgaagcgcg gaggctcgta ggggcaggcg accgccagta tttctgcctc
      541 ggcttgcacc gcctcgcgga cgcgccactc agacaggcgc acgtgtgcct tctcccaagt
      601 gaacccgtcc agccacatac cacctccacc acccccgcag cacagacctg tctccatatg
      661 atgagtcatc tccaccagct tcacaccagg aatggcttcc agcaagcggc gcggagcgtc
      721 atactcgccg tttgccctgc ccaggtagca gggatcatgg tatgtaactc ttgcttgaat
      781 ttcctcccgc aagtgtggtt tgagctgatc tagtctgtca accaggaatt gtgtgtaatg
      841 cttcaccggg tagacgatcc ccagctttgg gtattcattc ttaagggcat tgtatgcatg
      901 cgggtcggtc gttacaattt gactgaaggt atatttgtta aagacttgcc catttttctc
      961 agccaacatc tcaaacaacc cgcgctcacc tgccaatcgc tgagaatcgc catcactaaa
     1021 ttcttcgggt cctaatatgg cgaaatccac tccgatgaca ttaaacactt gggccacagc
     1081 ctggctgacc atttggacgc gcggatggaa ggaggcgtag tcgccaacaa accagaggac
     1141 atctacaggt cctcctacct ggctcatgat gcgcgccggc ggatcaatcc ccctagccca
     1201 catagctctt ttccttggag attcgccgag cgggttacca taacgttggg aattctcaag
     1261 ggcatcctgc aattcgctgg ggacattgcc agccaggacc atttcttctt tcgctcgtgt
     1321 caatagcccg ggagtgagcg gtatttttgc tgggcagacc tgagtgcagg catagcatga
     1381 gacgcacatc cagagcgatt catagtttat aacccgctca aacaaaccgg cgcgcagtgc
     1441 agcgatcatt ttgccaggag gatattccat tagatagcca aaggggcata ctccgctaca
     1501 ggttccacat tgcaggcaag cctgcatgcg ctgaccttcc ggcaacttaa acaagctgga
     1561 atagaacatg tcattgaagg aaggtctttg ggtaatggac tttgtagcca cagcaacacc
     1621 tcctgcttac actaggccag gcaaaataga tataaatagg taaaactccc ccggctcaac
     1681 acttggcgaa accaatccat gagcacgcga atgagaatcg tgctgaaaag gatttagaca
     1741 aattttgcct atgctattca gttttcaagg cgggaatgtg tggtgtgtta tgaaagcgag
     1801 gatgaaaccg cagaaacgat caccgagata ctctgccaag tgtgcgttct agggagctcg
     1861 cgctgatatg gggcatgtat tatgtcatct ttattatgtg aaatatatca caaataaacc
     1921 agtaacaaag cgcatattcg gtttgtgatt tttatcacat tatcatatct ttggcataaa
     1981 cagtgttcta gcccctacat agcattccct cccatatctt gtatgaccac cgcatcagtg
     2041 aagccaagct tctcttcgca actgatataa tccatcacga agcaaacccc cagacaggaa
     2101 tacgcatgaa ttcggtcaga ccccggtggt cattttatac ctatctgctt gttctctcca
     2161 tatttggaat cgcccttgta tttatcaaca cttccactta tggtccaggt atatccagtg
     2221 atgggctgca ttacctctcc accgccgaca gccttatcaa cggccgtggc tttaccaact
     2281 ttcacggcgg cgtatatatc ctgtggcctc ccttatatcc gctgattctg gcaggcctaa
     2341 gcattctgac aggtctcgac acgctgatca ttggttggat acttaacgcc gtggcctttg
     2401 gcgtgaccat cttcctttca ggggttttgc tgaaaacatg ttttcccgat caacctgcat
     2461 ggcaagcgct gggtgcggct cttgtattat tctttatgtc ctatgttgtg ttggcatcca
     2521 acatcggatc ggaccaattg tttgtcgttc tcgtactggg atcttttctg gcatgccatc
     2581 gctacatgac aacctcaaac cgtggcgctt tgtgggtctt gctgatcctg gttgccctgg
     2641 ctcctttgct gcgttttttg ggggtatgcg tggtagcagt tggcgccctg gctgttttcc
     2701 tgaaaaacat taagcagcct gttaattcta ctggcaagtc aattattttc ggcctggtgg
     2761 cctctctacc aaccttagtc tgggtaatcg gacgtaacta tcgcctatat ggcagcctga
     2821 caggaccgcg acaactgcat aatgccttaa tcattgaaaa tatcgcctac tgcacgcgcc
     2881 agatcttgcg ctggtttgtg ccgttgagcg tcatcgaccg gctacctctc tgggtcctcc
     2941 cggcgctcgt tctggtggtt ttgctgctgt ttgcccgcaa acgccactgg gca
//
LOCUS       DBNHOH010000135         1742 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_584135, whole genome shotgun sequence.
ACCESSION   DBNHOH010000135 DBNHOH010000000
VERSION     DBNHOH010000135.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_584135"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1064)
                     /locus_tag="AC16V7_02505"
     CDS             complement(105..1064)
                     /locus_tag="AC16V7_02505"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005985274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 -
                     branched-chain-amino-acid:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HIR6256018.1"
                     /translation="MGMESKYLWMDGKLVEYEKATIHFMTPALHYGVAAFEGVRSYAT
                     DNGPAIFRAKEHAARLLRSAKVLGFRELPYTEAELVEAMKLTVAANQFVECYIRPLIY
                     LTDGGWNLNVDAGKASLGISVWEWNNYLGDEALDKGIRANITSFTRHHPNVMMTKAKI
                     SGNYANSVLARTESMRLGFDEAIMLDPQGYVAECTGENLFLVRDGKIITPPTAAILEG
                     ITRDSIITLAKELGYEVIEQPISRDQLYVADEVFVCGTAAECIGLWEIDYRVIGNGKS
                     GPVTKDIQKAFHAAIRGQHPRSVEWLDYVNTAVPAFLPVDAMD"
     gene            complement(1146..>1742)
                     /locus_tag="AC16V7_02510"
     CDS             complement(1146..>1742)
                     /locus_tag="AC16V7_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010678330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HIR6256019.1"
                     /translation="DTMEYHDFEVRRILDMAFKLAQGRRNKVTSVDKANVLETSRLWR
                     QIASEMHSGYPELTLEHMLVDTAAMRLVSNPAGFDILVTENMFGDILTDEASVLSGSM
                     GLLPSASLGESGPGLYEPIHGSAPDIAGKGIANPIGTILSVAMMMRYSLGLEGEAAAI
                     EKAVNRVLADACRTADLGGSMTTTQMTSEIVQRLAADG"
BASE COUNT          384 a          518 c          447 g          393 t
ORIGIN      
        1 aaggtttcgc aagggaatgt ctgtacttga gtcccaaaat gctcgatgat ggctgcctcc
       61 gaataggccc caggctcgcc ttgaaaggct acacgcatat cttgtcaatc catggcgtcg
      121 actggaagga aggctggcac agccgtattc acataatcca gccactccac cgaacgcgga
      181 tgctggccgc ggatggcggc atggaacgcc ttttggatat ctttggtgac cgggccggat
      241 tttccattac ctatcacgcg gtaatcaatc tcccacagcc cgatgcactc ggcagcggtg
      301 ccgcacacga acacttcatc cgcaacatat agctggtcgc gcgagatggg ttgttcaatc
      361 acctcatagc ccagctcctt agccagggtg atgatcgaat cgcgcgtgat gccctccagg
      421 atggcggctg tgggtggggt aataattttt ccatcccgca ccaagaacag gttttcgccc
      481 gtgcattcgg ctacgtatcc ctgcgggtcc agcatgatcg cctcgtcaaa tcccaggcgc
      541 atggattcgg tgcgcgccag cacgctattg gcatagttac cgctgatttt ggccttggtc
      601 atcatcacgt tggggtggtg tcgggtaaac gaggtgatat tggcgcggat acccttatcc
      661 agggcttcgt cgcccagata gttgttccat tcccacacag agatacccaa gctggctttt
      721 ccagcatcca cattcaggtt ccagcccccg tctgtcaggt agatcagtgg gcggatatag
      781 cactccacga actggttagc agccaccgtc agcttcatcg cctccaccag ctcagcttcg
      841 gtatatggca actcacgaaa gcccaggacc ttagcagatc tcagcagccg cgccgcatgt
      901 tccttggcgc ggaatatcgc cggcccatta tcggtggcat aactgcgcac gccctcgaag
      961 gctgccacac cgtaatgcag agccggggtc ataaagtgga tagtggcttt ctcgtactca
     1021 accagcttgc catccatcca gaggtatttt gattccatgc ccatcgcttg tttctccttt
     1081 agataaaaat tagagcttaa tcgttgaagg aaaagtctca tcaacgcgga tgatcacctt
     1141 cgagatcatc catcggcagc aagacgctgc acaatctcgc tagtcatctg ggtagtggtc
     1201 atcgacccac ccaagtcagc cgtacggcaa gcgtcagcca atacgcgatt aacagccttc
     1261 tcgattgcgg cggcttcgcc ttccaatccc agagagtagc gcatcatcat cgccacgctc
     1321 aggatggtgc cgatcggatt cgcgatccct ttgccggcaa tatcgggcgc tgacccgtgg
     1381 attggctcgt acagccccgg cccgctctca cccaaagagg ctgagggtaa cagccccatc
     1441 gagccgctca agaccgacgc ttcgtcggta agtatgtcgc caaacatgtt ctcagtcacc
     1501 aggatatcga aaccggccgg gttcgagacc aggcgcatgg cagctgtatc aaccagcatg
     1561 tgctcaagag ttaactccgg gtagccggag tgcatctctg aagcgatctg ccgccataaa
     1621 cgcgacgtct ccagcacatt ggctttatcc accgaggtta ccttattgcg ccgtccctga
     1681 gcgagtttga aggccatatc caggatgcgc cgtacttcaa aatcgtggta ttccatagta
     1741 tc
//
LOCUS       DBNHOH010000136         1338 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_969485, whole genome shotgun sequence.
ACCESSION   DBNHOH010000136 DBNHOH010000000
VERSION     DBNHOH010000136.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_969485"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="AC16V7_02515"
     CDS             complement(<1..752)
                     /locus_tag="AC16V7_02515"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HIR6256020.1"
                     /translation="MAQKCVKLQPQREEGWLILGALASPKGSVNYLQHALSINPASQR
                     ARKGMHWAIQRLRKNNTAADAPAPIPRTRVAATITSDSMARQRPLLMPWAIAITLLVL
                     GLAIWFGSPTFSLAFNNGKQLSLAQVQLSKQTRTPTPTATFTPTPTSTSTPTPTITPS
                     PTPTETPSPTPTDTPKPTKKPKKNNVAKENYNYPGRPRGVDGNERWIDIDLSSQRVYV
                     YQGDQLVNKFVVSTGTWQTPTVKGTYKIYVKYR"
     gene            complement(836..>1338)
                     /locus_tag="AC16V7_02520"
     CDS             complement(836..>1338)
                     /locus_tag="AC16V7_02520"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR6256021.1"
                     /translation="DGLTEASVNEKVAYLVQKDLVDNGYDVEVLNEFDPQLSDYQASV
                     LVSIHSDSCDYVNNQATGFKVSSALANPHPDRAARLTACLKNRYGIVTGLPLHNSITI
                     DMTSYHAFAEISDNTTAAIIEIGFLNLDRELLTQKSDLVAKGIFDGILCYINNEDLIV
                     NATPTP"
BASE COUNT          293 a          287 c          378 g          380 t
ORIGIN      
        1 cgatatttga cgtatatctt gtaagtgccc ttcaccgtgg gtgtttgcca tgtaccggta
       61 gatactacaa atttattgac gagttgatcg ccttgataga cataaacccg ttgcgagctc
      121 aaatcaatgt cgatccaacg ctcattcccg tccacgccac gaggtcttcc agggtaattg
      181 taattttctt ttgcaacgtt atttttcttg ggttttttgg tgggttttgg tgtatcagtt
      241 ggcgtagggg agggtgtttc cgttggagtt ggagagggtg taatggtggg ggtgggtgtc
      301 gaagtcgaag ttggcgtagg agtaaatgta gcagtggggg tcggggttcg tgtttgcttg
      361 cttagctgca cctgggctag gcttaattgt tttccattgt tgaacgccaa ggaaaaagtt
      421 ggcgatccga accatatggc taatcccagc accagaagtg taattgctat agcccagggc
      481 atgagcaggg ggcgctggcg cgccattgaa tctgaggtga tcgttgctgc gacccgggta
      541 cgtgggatag gggcaggtgc atccgccgcc gtattgtttt tgcgcaggcg ttgaattgcc
      601 caatgcattc cctttcgggc tcgctgggaa gctggattga tgcttaacgc gtgttggaga
      661 tagttaacac tgccttttgg gctggctaga gcccccaata tcagccaacc ctcttcccgt
      721 tgaggctgaa gcttgacaca cttttgtgcc aatcggcgag cttctaaccg atttccatat
      781 ctcagcgcgc gctgtgcctc actgagcaga acaccataag attcaacacg agaggtcatg
      841 gcgttggtgt ggcgttaacg ataagatctt cgttgttgat ataacagaga ataccgtcaa
      901 aaatgccttt ggccaccagg tcggattttt gggtcagcag ctcgcggtct aaatttagga
      961 agcctatctc gatgatcgcg gcggtggtgt tatcgctaat ctcggcgaaa gcgtgatagc
     1021 tggtcatgtc gatggtgatg ctattgtgca atggaagtcc ggtcactatg ccatagcgat
     1081 tttttaaaca ggctgtcagg cgggctgcac gatctggatg gggattggct aaagcagaag
     1141 ataccttgaa cccagtagct tgattattta cataatcaca cgagtcggaa tggattgaaa
     1201 ccagtacgga ggcctggtag tcgcttaatt ggggatcaaa ctcattgagc acttcaacgt
     1261 cgtatccatt gtctaccaga tctttttgta caagataggc tactttctcg ttgacgcttg
     1321 cctctgtcag accgtctg
//
LOCUS       DBNHOH010000137         3095 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_584337, whole genome shotgun sequence.
ACCESSION   DBNHOH010000137 DBNHOH010000000
VERSION     DBNHOH010000137.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_584337"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /locus_tag="AC16V7_02525"
     CDS             <1..1139
                     /locus_tag="AC16V7_02525"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR6256022.1"
                     /translation="QAEGAYAVLARAQELEAQGREILHLEIGQPDFQTFPHISERGIQ
                     AIRDGQTRYNPPGGLPALREAIAADAGTRRGIEFSPTQVVVGPGAKPGLFFPTLALIE
                     PGDEVIYPDPGFPTYAAMIEVAGGKPVPVPLVEGNNFSFDLQAFSERLSQRTKLIILN
                     SPANPTGGVMPLADLEHIADATQAYDCWVISDEIYARLIYDKRPAPSIISLPGMKERT
                     ILVDGFSKTYAMTGWRLGFGIMPEALAERVELLLTHSVGCTATFTQVAGIEALSGDQE
                     MVDQVTVEYQRRRNRIVAGLNAIPGVRCQLPAGAFYAFPNIKSFGLSSHQLAARLLDE
                     GGVAVLAGTDFGPGGEGYLRLCYATSPEIIDRALARMAAFLGSL"
     gene            1156..2295
                     /locus_tag="AC16V7_02530"
     CDS             1156..2295
                     /locus_tag="AC16V7_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256023.1"
                     /translation="MFRVFGRSSVSEFLADRIVYRNLEPADPRLPSLESLRVRLGLPE
                     GQIPRKSEPDYARVIVELLQQAQRQDAPHTAIRKLVLIGDTRLLDGTAFENICLAGGW
                     PGLAFIGSETRQPPAVEIIPLANEQSLYLANRWAAMQEFDLYCEGRGQPIGEDTAVVI
                     DLDKTAIGARGRNSQTIDQARVRAVHITVASILGASFNAAAFQRAYDQLNQPEFHPFT
                     ADNQDYLAYICLVLGSGLYDLEDVVRQVRNGKMTSFRQFIDHAEAQAVNLPSALEQVH
                     RQIYANVVRGDPTPFKSFRRNEFLETVGRMGILGDADPTEKLLAEEIVITQEVRAMAL
                     EWRERGALLFGLSDKPDEASLPTPEQAARGYRPIHQTETHAVGTG"
     gene            2698..>3095
                     /locus_tag="AC16V7_02535"
     CDS             2698..>3095
                     /locus_tag="AC16V7_02535"
                     /inference="COORDINATES: protein motif:HMM:NF024867.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6256024.1"
                     /translation="MSAIELACISGANGAGKSSLLDAITWTLFGVARKRDDTLINAQS
                     NAAEVSLVFAYEGNIYRVQRSLPRGKTTQLEFQIKQNGGQHATINPQLETATWKPLSE
                     RTLRETQARIEQTLRMDYETFVNASFFLQG"
BASE COUNT          639 a          940 c          900 g          616 t
ORIGIN      
        1 tccaggccga gggggcatac gctgtgctgg cccgcgccca ggagctggag gcgcaggggc
       61 gcgaaatcct gcacctggag atcggccagc ccgatttcca gaccttcccg catatcagcg
      121 agcgcggtat ccaggctatc cgggatgggc agacgcgcta taacccgccg ggcggcttgc
      181 ccgccttgag agaggctatc gccgccgacg ccggtacgcg ccgtggtatt gaattttccc
      241 caacgcaggt ggtggtcggt ccaggagcca aaccagggtt gttcttcccc acgctggccc
      301 tgatcgagcc aggcgacgag gtaatctacc ccgatccggg cttcccgacc tacgcggcga
      361 tgatcgaggt cgccggtggg aagccggtgc cagtcccgct ggtggagggt aacaatttct
      421 ccttcgattt gcaagccttc agcgagcgcc tcagccagcg cacgaagttg atcatcctga
      481 actcgcctgc caatcctacc ggcggggtga tgcccctggc ggacctagag cacatcgccg
      541 acgccacgca agcttacgat tgctgggtga tctcggatga gatctacgcc cggttgattt
      601 atgacaagcg cccagctccc agcatcatct ccctgcctgg catgaaagag cgcaccatcc
      661 ttgtggatgg cttctccaag acctatgcca tgactggctg gcggttggga tttgggatca
      721 tgccagaggc cttggcagag cgtgtcgagc tgctgctgac ccactccgtt ggctgcactg
      781 ctaccttcac ccaggttgcc ggtatcgagg cactgagcgg cgaccaggag atggtagacc
      841 aggtgacagt cgaataccag cgccgccgca atcgcatcgt cgccggattg aacgccatcc
      901 ctggggtgcg ctgccagctg ccggcggggg cattctacgc tttcccaaat atcaagtcat
      961 ttgggctctc atcacaccag ctggcagccc gcttgctgga cgaagggggc gtggctgtcc
     1021 tggcagggac agactttggc cccggcgggg agggttacct gcgcctgtgc tacgccacct
     1081 cgccagagat tatcgatcgg gcactggcgc gaatggctgc ctttttgggg tcgttataaa
     1141 gggtacagaa tatcgatgtt tcgtgttttt ggccgttctt cagtcagtga gttcctggcc
     1201 gaccggattg tatatcgcaa cctggagcca gccgaccccc gtttgccatc tctggagagc
     1261 ctgcgcgtgc ggctcggact gcccgaaggg cagataccgc gcaagagcga accagactac
     1321 gcccgcgtta tcgtggaact gctacagcag gcacaaaggc aagatgcccc gcataccgcc
     1381 atccgcaagc tggtcctgat cggagacacg cgcctgctag atggcacggc ttttgagaat
     1441 atctgtctgg caggcggctg gcccgggctg gccttcatcg gctcggagac caggcagcca
     1501 cccgcggtag aaataatccc cttggccaat gaacagtccc tctatctcgc taaccgctgg
     1561 gcggctatgc aggaattcga tctgtactgc gaagggcgtg ggcaacccat cggcgaggac
     1621 accgcagtgg tcatcgacct ggataagacc gccatcggtg cgcgcgggcg caacagccaa
     1681 actatcgacc aggcgcgggt gagggccgta cacatcaccg tggcaagcat cctgggagcg
     1741 tccttcaacg cagcagcttt ccagcgcgct tacgaccagc tcaatcagcc ggaatttcac
     1801 cccttcaccg ccgacaacca ggattacctg gcttacatct gcctggtact gggaagcggg
     1861 ttgtacgacc tggaggatgt cgtccggcag gtgcggaatg gaaaaatgac ctcattccgc
     1921 cagtttattg atcatgcgga agcgcaggca gtcaatttgc cctcagcgct ggagcaagta
     1981 caccgccaga tctacgccaa tgtggtccgc ggcgacccca cacctttcaa atccttccgc
     2041 cgcaacgagt tcctggagac ggtggggcgc atggggattc ttggcgatgc tgacccgacc
     2101 gagaagctac tggcagagga gatcgtcatc acgcaggaag tgcgcgccat ggcgctggag
     2161 tggcgcgagc ggggggcgct gctcttcggc ctttcggaca agcccgacga ggcttcgctg
     2221 cccacgccag agcaggcagc ccgcggttac cgccctatcc atcaaaccga gacacatgcc
     2281 gtaggcacag gctagttcca tgcctcccta gttcttgcca ggggggaaac acatttctcc
     2341 gccgatcccc agggcaattg catatgcccg gaagggcatg tgcaatctcg caaaaagagt
     2401 gtttggggtg ttttggtgtg ggcatacccc caccaaaaca ccccattttc tctcctcagt
     2461 ggtggggaat cgcccaaatg cgattgctct acgccaatcc caagggggta aggtaaataa
     2521 aaagttttgc agtacaatac ataccgcact ctgcagataa attgcttggc tggttgtctt
     2581 ccgggcatct cgacccgcat ctaaaaatgc caagctccgg ggtcaaatcg atccatgatt
     2641 cctctttctc tatctatctc tggttttctc tcataccgcg acccggttga gctggatttg
     2701 tctgctatag aattggcctg catctccggg gccaacgggg ctggcaaatc gtccctgctg
     2761 gatgcgatca cctggacact tttcggcgta gcgcgcaagc gcgacgatac gctgatcaac
     2821 gcccaatcga atgccgccga ggtcagtctg gtctttgctt acgagggtaa catctaccgc
     2881 gtacagcgca gcctgccgcg aggtaaaacg acccaactcg aattccaaat taaacaaaat
     2941 ggtggacagc acgcaacgat caacccccag ttagagactg ccacctggaa accattgtcg
     3001 gagaggacac tgcgcgaaac ccaggcgcgc atcgagcaga cgctgcgcat ggattacgaa
     3061 acatttgtca acgcctcgtt ctttttgcag ggcaa
//
LOCUS       DBNHOH010000138         2014 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_327898, whole genome shotgun sequence.
ACCESSION   DBNHOH010000138 DBNHOH010000000
VERSION     DBNHOH010000138.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_327898"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="AC16V7_02540"
     CDS             <1..858
                     /locus_tag="AC16V7_02540"
                     /inference="COORDINATES: protein motif:HMM:NF015956.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HIR6256025.1"
                     /translation="ASPEALAGTLRLYGNDLLRLMTRMETQGLLLSADGGLRLTPDGE
                     RLALHVVRAHRLLERYLADEARLPLEKVHSEAERREHGLTPEQLDDLAASLGYPDLDP
                     HGDPIPDQQGEMVPAAGTSLTSWPAGVQGRIAHLEDEPPLAYDQILAEGLRLGQVVHI
                     LETTPARIVLSDGENEYRLAPAVAANIYVSPLPESLESQSGVMPLSELPDKMEAEVVA
                     LDEACQGFTRRRLLDLGMTPGVRLVAEMRNFFGDPRAYRVRGTLIALRREQAASIWVR
                     PVERQAAEG"
     gene            925..1737
                     /locus_tag="AC16V7_02545"
     CDS             925..1737
                     /locus_tag="AC16V7_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HIR6256026.1"
                     /translation="MSEPCENCPANVHLAQMGLKLDKFDRVVALAGNPNTGKSTLFNA
                     LTGLRQHTGNWPGKTVTRAEGGFEFNGVRYKLVDLPGTYSLLSASQDEEVARDFILFG
                     QPDCTIVVTDATALERNLNLVLQVLEITDRVVVAVNLMDEAQRKGLEVDVRSLARDLG
                     VPAVPVTARTGEGIHGLLSAVEGLISGEIPTKPRRVEGTPEFQRAVNELVPMIESLAP
                     GVPNARWLAIRLLDGDTRVRQAILNGELAELVAHQRQADVSFSRKIALEGTQ"
     gene            1829..>2014
                     /locus_tag="AC16V7_02550"
     CDS             1829..>2014
                     /locus_tag="AC16V7_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256027.1"
                     /translation="METSHLQPSELLAQADTLRRGLSAGFRDETVRSIYNEAERIARR
                     AVKTKADQRWDFDQRIDR"
BASE COUNT          406 a          575 c          640 g          393 t
ORIGIN      
        1 gcctcgccag aagcgttggc gggcacgttg cggctctatg gaaacgacct gctgcgcttg
       61 atgacccgca tggaaaccca gggattgctg ctatcggcag atggcgggct gcgcttgacc
      121 ccagacggtg agcgcttggc gctgcatgtg gtgcgtgccc accgcctttt agagcgctat
      181 ctggctgacg aggcccgcct gcctctggag aaagttcact cagaagccga gcgccgcgag
      241 cacggtctga cccccgagca gcttgacgat ctcgcagcct ccttgggcta tccagacctg
      301 gacccgcacg gcgatccaat acctgaccaa cagggcgaga tggtccccgc agccggcacg
      361 tccctgactt cctggcctgc tggcgttcag gggcgcattg ctcatctgga ggatgagcct
      421 cccctggctt acgaccagat tctggcggag gggttgcgtc tggggcaggt ggtgcatatc
      481 ttagagacca cccctgcacg aatagtgctg agcgacggtg agaacgaata ccgccttgcc
      541 ccagcggtgg ccgccaacat ttacgtgagc ccgcttccag agtcccttga atcgcaatct
      601 ggggtgatgc ccctctcgga gctgcccgat aagatggaag ctgaagtggt ggcgctggac
      661 gaagcctgcc agggttttac ccgccggcgc ttgctagatc tggggatgac acctggagtg
      721 cgcctggttg ccgagatgcg caatttcttc ggcgacccgc gtgcctaccg ggtgcgcggt
      781 acactgatcg ctctgcgccg cgagcaagca gccagcatat gggtcaggcc tgttgagcgg
      841 caggctgctg aaggataatt gagcagggag tcaaactgca atctaaaaga ttaacatacg
      901 acattatgta attaggatgt atctatgagc gaaccttgtg agaactgccc tgccaacgtg
      961 catttggctc aaatgggcct aaagctggat aaattcgacc gggtggtagc cctggcgggc
     1021 aaccccaata ccggaaaatc gaccttgttt aacgccttga ccggcttgcg ccagcatacc
     1081 ggcaactggc cgggcaagac ggtgacccgc gccgaaggcg ggtttgagtt taatggcgtg
     1141 cgctataagc tggtggattt gcctggcacg tattcgctgc tatccgcatc ccaagatgag
     1201 gaagtggcgc gtgattttat cctctttggg cagcctgact gcactattgt agttactgac
     1261 gccactgccc tggagcgcaa tctgaacctg gtgctgcaag tgctggagat caccgaccgg
     1321 gtggtggtag ctgtcaactt gatggacgag gctcagcgca agggtctgga agttgatgtg
     1381 cgcagcctgg cgcgcgacct gggagtgccc gccgtaccgg tgacagcccg taccggggag
     1441 ggcatccacg gactgcttag cgcggtggag ggattgatca gtggtgaaat ccccaccaag
     1501 ccgcgccgcg tggagggcac gcccgagttc cagcgtgcag tcaacgagct ggtgccgatg
     1561 atagagagtc tagcgccagg tgtgcccaat gcgcgctggc tggctatccg cttgctggat
     1621 ggcgacacgc gcgtgcgcca ggccatactc aacggtgagc tggctgaact ggtcgcccac
     1681 caacgccagg cagacgtgag ctttagccgt aagatcgccc tggaagggac gcaataaagt
     1741 accaagtacc aagtgtcagg taccacgttt tgtgtggact gagcgcttgg aacttggaac
     1801 atggaacttg gtacttggaa tggagaccat ggaaacatcc caccttcaac cctccgaact
     1861 gctcgcgcag gcagatacct tgcgccgcgg cctgagcgct ggattccgtg atgagacggt
     1921 gcggtcgatc tacaacgagg ctgagcgcat cgcccggcgc gccgtgaaga ccaaagcgga
     1981 ccagcgctgg gactttgacc agcgcatcga ccgg
//
LOCUS       DBNHOH010000139         1669 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_969587, whole genome shotgun sequence.
ACCESSION   DBNHOH010000139 DBNHOH010000000
VERSION     DBNHOH010000139.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_969587"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="AC16V7_02555"
     CDS             <1..682
                     /locus_tag="AC16V7_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003913 - DNA photolyase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HIR6256028.1"
                     /translation="IAALESRVSAGDPQALKGVDTWINELVWREFYIAILYHFPHVLK
                     TAFNPSLRNVPWRDAPQDLQAWKAGLTGYPVVDAGMRQLAGTGWMHNRARMIVASFLV
                     KDLLVNWQDGERWFMQSLVDGDPAANNGGWQWTAGVGTDAAPYFRIFNPVLQGFKFDP
                     SGSYVRRWVPELVKVPEKWIHRPWEMPQEAQLAVGFKPGKDYPLPIVDHALARQRVLE
                     AYKRAAKG"
     gene            complement(745..1434)
                     /locus_tag="AC16V7_02560"
     CDS             complement(745..1434)
                     /locus_tag="AC16V7_02560"
                     /inference="COORDINATES: protein motif:HMM:NF025636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6256029.1"
                     /translation="MSTTNSAERSLLNNFRKSSKSGSKERKVDRRVYRTRRALREAFF
                     ALILEKGYEAVTIEEITNRADLGRTTFYLHYRDKEDLLMESIGELVDDLVERMSHLPL
                     GDWKIDLNFSNSQAPPAITLPFQHVAQNAQLYRIILRGEGNFSITARLREIIVQASGE
                     IIQVMLKKEKLAINPQLPLEVFMNYLAASWLGLITWWLEKEMPYTPEQMATMFQMMFI
                     RGARDVLGLTS"
BASE COUNT          376 a          425 c          460 g          408 t
ORIGIN      
        1 gattgcggcc ttggaaagcc gcgtttctgc tggcgatcca caagcgctta aaggggtgga
       61 tacctggatt aatgagctcg tctggcgcga attttatata gctatcctgt accatttccc
      121 ccacgtattg aagacagcct tcaacccttc cttgcgcaat gtcccctggc gagatgctcc
      181 ccaagacctg caggcttgga aagctggttt aaccggctac cctgtcgtgg acgcgggtat
      241 gcggcagcta gcaggtaccg gctggatgca caaccgggcg cgcatgattg tggcctcgtt
      301 cctggtgaag gatttactgg tcaactggca ggatggtgag cgctggttca tgcaatcgct
      361 ggtcgatggt gacccagcag ccaacaatgg cggctggcag tggaccgccg gtgtgggcac
      421 cgatgccgca ccctattttc ggattttcaa cccggtatta caaggcttta agtttgaccc
      481 ctctggttcc tatgtccgcc gctgggtgcc tgaattggtg aaggttcctg agaaatggat
      541 tcatcgcccc tgggagatgc cccaggaggc ccagcttgcc gttggcttta agcccggcaa
      601 agattacccg ctgcccattg tggatcacgc gctcgctcgc cagcgggtac tggaggcata
      661 caagcgagcc gctaagggtt gaatatttag ttttctgtag aaacgactac ggggtaagcc
      721 taaaaaagca tcactgcaag cctgctatga tgtcaacccc aatacatctc gagctccacg
      781 gatgaacatc atttggaaca tggtcgccat ctgctcgggt gtatagggca tctccttctc
      841 cagccaccaa gtgatcagcc ccaaccagga agctgccaga taattcatga agacctccag
      901 tggcagttga gggttgatcg ccagcttctc ttttttcagc atcacctgga tgatttcacc
      961 ggaagcttgg acaatgattt cgcgcaggcg tgctgttatg gagaaattgc cttcgccgcg
     1021 caggatgata cgatagagct gtgcgttttg ggcaacgtgc tggaacggca acgtgatggc
     1081 tgggggcgct tggctgttag aaaagttgag atcgattttc caatcaccta aggggaggtg
     1141 agacattcgt tcaaccagat catcgaccag ctcgccaatg ctttccatga gcaggtcttc
     1201 tttgtcgcgg taatggaggt aaaatgtggt gcgtcctagg tccgcccggt tggtgatctc
     1261 ctcgatggtg accgcttcgt agcccttctc aagaatcagc gcaaagaatg cttctcgcag
     1321 ggcccggcgg gttcgataga cgcgccgatc tactttgcgc tccttggagc cagacttcga
     1381 cgatttgcga aaattgttca gtaatgaacg ctcagcagaa ttggtggttg acaagacgca
     1441 agccctccat ataattacta gacgtatgtc tattatacta cagcgtgttt attaatggaa
     1501 gatgagagaa aaaatttcta aaaatggaaa tggtcgaact tcaattgcta acccaccaca
     1561 tccgctcaat ggcaacgggc gcaccccgct tgttcgcctg cggggagtta gcaagagcta
     1621 tcagaccgcg gctggagatt tcctggcttt aagcgggatc gatttggat
//
LOCUS       DBNHOH010000140         2953 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1610105, whole genome shotgun sequence.
ACCESSION   DBNHOH010000140 DBNHOH010000000
VERSION     DBNHOH010000140.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1610105"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="AC16V7_02565"
     CDS             <1..495
                     /locus_tag="AC16V7_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR6256030.1"
                     /translation="PDIIVTGKGLSGGIYPITATMYREHLNPFMHDNPFIHVSTFGGA
                     EIGCHVALEVLDIMEEPGFLKHVRSLAFQFKEGFARLQDNHPVIMVEPRQRGLMMGLK
                     LANADYGPIMTQLGFHAGLLTIFANHDPSVIQILPPLIIQPGEVEQVLNILDEMLGML
                     EMMV"
     gene            603..2246
                     /locus_tag="AC16V7_02570"
     CDS             603..2246
                     /locus_tag="AC16V7_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256031.1"
                     /translation="MVIILVAVVGASLAYLYPSPVEFPMDDAYIHMVYAQNLVDKGSL
                     MFNDPEEKGVGTTSASWVLLLAAGYRLGIPLHVVLKILGVASLIGLGVGLYLLLVRYW
                     AAVPALIATLFVILSGHMLWFALSGMETILFLTIGVFALLFYRNERWIWLGIFLGLLT
                     LTRPEGILLAVSIGLVDIWRRKALAKGLVMAGIVVFLMCAPWYGYILARTGNLLPTSG
                     IGKRVSSQVGMHLLLQQNEALSVFAKYPSLLYPFVWLGYLLEYVLGGVALPPPDLRLV
                     TSIASETYSISAWALLGFVGVVAPLVWRGSRQIFSGLRQPNSILGEKSALMLVCLIWV
                     VLHNLSYMSFLPFPGTASRYGALNHIVLWIVLLSGLFGFWHRRGIFLWLAAGLSVIAV
                     ANMGYWNRVYDANIDHMVSVRIQAARYINRVIPEEELCAAMDVGALRFYSQRPIVDLG
                     GLIDPELGKLFLSGDLDRHLVEMGVTCLILPGRAGESTDGWFDIAKELGLLESPFVRL
                     HQEKVFEMDRKRWLFGYQPTRNYQATVTIYRMESLCSMC"
     gene            complement(2250..>2953)
                     /locus_tag="AC16V7_02575"
     CDS             complement(2250..>2953)
                     /locus_tag="AC16V7_02575"
                     /inference="COORDINATES: protein motif:HMM:NF024660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR6256032.1"
                     /translation="SLRFMTPTQILMLRVEFHSHTMYSKDSLARPERLLSACRRKGID
                     RLVVTDHNTIAGAMQCQELDPERVIIGEEILTQSGEILAAFVQEHIPARLTAQETIAH
                     LRAQGAFISVSHPFDISRKGHWQLAQLLEIAPLVDAIETFNARCFLPSFNTQAQDFAH
                     QQKLLGTAGSDAHTTYELGRATMLMPDFHDAESFKTALTQAQFETSLSSPWIHLSSRY
                     ATWVKKMQRKRSQNR"
BASE COUNT          621 a          665 c          823 g          844 t
ORIGIN      
        1 cccgatatca ttgtgacagg caaaggatta tcagggggca tttacccgat aaccgccacc
       61 atgtaccggg agcatctcaa cccgtttatg cacgataatc cctttatcca tgtatccact
      121 ttcggaggag cggagattgg ttgtcatgta gctttggaag tgctggacat catggaggag
      181 cctggttttt tgaagcatgt tcgctcgtta gcatttcaat tcaaggaggg atttgcccgt
      241 ctgcaagata atcaccctgt tataatggtt gagcctcgcc aacgtggtct gatgatggga
      301 ttgaagctag ctaacgccga ctatggaccc atcatgacgc aattgggttt tcacgctggc
      361 ttgctgacta tctttgccaa ccatgatccc tcagtaatcc agatcctgcc gccattaatc
      421 atccagcctg gcgaagtcga acaggttctg aatatattgg atgaaatgtt gggcatgttg
      481 gagatgatgg tttaagcccg ccgtgcacat acttgaggag ttgatctatc gcccactcta
      541 gtttagaaat aagacgcgcc caaaccaagc gaaaggcttt tctaaccagg cactggacac
      601 ccttggtaat tatcctggtc gcggtggttg gtgcttcttt agcttatctt tatccatcgc
      661 ctgttgaatt tccgatggat gatgcctata tccacatggt gtacgcacaa aacctggtgg
      721 ataagggtag tttgatgttc aacgatcctg aagaaaaggg tgttggcaca acaagtgcgt
      781 cttgggtatt gctcttggca gcgggatatc gcctgggcat ccctctccat gtggtcttga
      841 aaatactggg ggttgcatca ctgattggtc tgggtgtggg gttgtacctc cttttggttc
      901 gatattgggc agcggtcccg gcgcttatcg cgacgctatt tgtcattcta tctggacata
      961 tgctctggtt tgccttgagt ggcatggaga cgatcctttt tttgaccatt ggggtatttg
     1021 cgttactttt ctatcgaaat gagcgttgga tctggttggg tatcttcctt gggttactga
     1081 ccctcacccg ccccgagggg atactgctag cggttagtat aggactagtc gatatttggc
     1141 gtcgtaaagc tctggcgaaa gggcttgtga tggctggtat tgtggttttc ctgatgtgtg
     1201 caccctggta tggttacatc ctggcgcgca ctggaaatct actgcctacc agtggcatag
     1261 gcaaacgtgt aagcagtcaa gttggtatgc acctgctact gcagcaaaac gaagcgttgt
     1321 ccgtatttgc gaaataccct tccctacttt acccttttgt ctggttggga tatcttcttg
     1381 aatacgtatt aggcggggta gcgctgccgc ccccagattt gcgcctggtt acctcgattg
     1441 ccagcgaaac ttattcaatt tctgcctggg ccttgcttgg ctttgtggga gtagtcgcac
     1501 ctctggtttg gagaggttct cgtcaaatat ttagcggact tcgccagccc aattcgatac
     1561 tgggtgaaaa atctgcatta atgttggtct gcttgatctg ggttgttctc cataacctga
     1621 gctacatgtc tttcctgcca tttccaggga cagccagccg gtatggggct ctgaatcaca
     1681 tcgtgttatg gattgtattg ctctctggct tatttggttt ttggcaccgg cgaggtatct
     1741 tcttgtggct ggcagctggg ttaagcgtta tcgccgttgc caatatgggc tattggaatc
     1801 gcgtgtacga tgcgaatatc gaccacatgg tgagcgtacg cattcaggct gcacgatata
     1861 tcaatagggt gataccggag gaagagcttt gtgcagcaat ggatgttgga gcgctgcgct
     1921 tctacagcca gcgacctatt gtggacttag gcggtttgat tgatcctgag ttgggaaagc
     1981 tatttttgag tggggatttg gatcgtcatt tagttgagat gggcgtcaca tgcttgattt
     2041 tgccagggcg agcaggggag agcaccgatg gctggtttga tattgccaag gaactgggct
     2101 tactcgagag cccctttgtt cgtttgcacc aagagaaggt ctttgagatg gatcgcaaac
     2161 gctggctctt tggatatcaa cctacacgca attaccaggc cacggtgacg atctaccgca
     2221 tggaatcgtt atgctctatg tgctagacat taccggtttt gtgatcgctt gcgctgcatt
     2281 ttcttcaccc aggtcgcgta tcgtgagctt agatgaatcc agggggagga caggcttgtc
     2341 tcgaattggg cttgtgtcag ggcggttttg aagctttctg catcgtggaa atccggcatc
     2401 agcatagtag cccgtcccaa ttcataggta gtatgcgcgt cggaaccagc cgtgcccaat
     2461 agcttttgct ggtgagcaaa atcctgggct tgtgtgttga aagaaggcag gaagcaacgc
     2521 gcgttaaagg tttcgatggc atccactagc ggggcgatct cgagcagttg ggcgagttgc
     2581 cagtgtccct tgcgcgatat atcaaatggg tgcgagacgc taatgaaagc gccttgggcg
     2641 cgcaggtgtg caatggtctc ctgggcggtt aaccgtgcag ggatatgttc ttggacaaag
     2701 gctgccagga tctctccgga ttgagtcagg atttcctcgc caatgatgac ccgctccgga
     2761 tccagctcct gacattgcat tgcacctgcg atggtgttat ggtcagtgac caccagccgg
     2821 tcaatgccct tgcgcctgca ggctgagagc aatctctctg gtcttgccag gctatccttt
     2881 gaatacattg tatggctgtg gaactcaacg cgaagcatga ggatttgggt tggggtcata
     2941 aatcgaaggg ata
//
LOCUS       DBNHOH010000141         1763 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1675020, whole genome shotgun sequence.
ACCESSION   DBNHOH010000141 DBNHOH010000000
VERSION     DBNHOH010000141.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1675020"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..982)
                     /locus_tag="AC16V7_02580"
     CDS             complement(161..982)
                     /locus_tag="AC16V7_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_095042061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HIR6256033.1"
                     /translation="MDTRIVAVYCLCDDMLKALHHRDDPQCQMTDAEVMTTAIVAALD
                     YGGNHEKARRMLQAEGYVPNMLGKSRFNRRWHRNADLFLTLFNLLGETWKQLNSNSIY
                     VIDSFPVAACDNYRIQRCQIYQEEDFRGYQASKRRFFYGLKIHLMITETGQPVEFFLT
                     PGSYSDTSALKLYDFDLPEGAKLTGDKAYNDYEVEDVINQAGISFIPLRKKNSKRPLP
                     PWVHYLRASYRKLIETTGSLIEQLLPKHIHAVTSRGFELKVALFVLACSINYLVG"
     gene            1120..>1763
                     /locus_tag="AC16V7_02585"
     CDS             1120..>1763
                     /locus_tag="AC16V7_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HIR6256034.1"
                     /translation="MDAKTLQTLEYPKILDRLASHCAFPASVDKAHQLLPTKDIEEAR
                     HRQASTREALSLLATHPDLSIGGARDVRPAVDLANHGGILLPNDLLDIKSTLIAARTL
                     ERLFERLGEQYPHITEIALRMSPPSGMIDSISRAISERGEILDTASEKLANIRRDLRV
                     VHDRLLAKLQRMVGDPHNAQFLQESLITQRDGRYVLPLRSEFKGRIKSIVHDQSA"
BASE COUNT          456 a          454 c          431 g          422 t
ORIGIN      
        1 gatttcaaaa ataatagttg tcatgggaag ccactctcgt ttggttttcg atagagttgg
       61 ggctacggtc ctgggagaag tatgtcggga agatgttgcg cgctcgatag ccttgaattt
      121 actggaattc ggttcctaat ataatattta acccaagttg ctacccaaca agatagttaa
      181 tagaacaggc aagcacgaag agagccactt tgagttcaaa accacgggaa gtaacggcat
      241 gaatatgctt cggcaagagc tgctcgatca agctacctgt tgtttcaata agcttgcggt
      301 aagaagctct caagtagtga acccaagggg ggagggggcg cttagagttc ttcttccgca
      361 aagggataaa ggagatgcca gcttggttga tgacatcttc tacctcataa tcgttatacg
      421 ccttgtcacc ggtgagcttg gcgccttcag gcaggtcaaa gtcatacagc ttcaaggcac
      481 tcgtatcgct gtaactgccc ggagtgagga aaaactcgac cggttgaccg gtctcggtga
      541 tcatcagatg gatcttcaga ccataaaaga aacgtctctt gctggcctgg tagcctcgaa
      601 aatcttcttc ttggtaaatc tggcagcgct gaatgcgata gttgtcgcaa gccgccactg
      661 gaaagctatc gatcacgtag atcgagttgc tgttcaactg cttccaggtt tcacccagca
      721 ggttgaacag ggtcagaaac aaatcggcat tgcgatgcca acgacgattg aaacgacttt
      781 ttcccaacat attgggaacg tatccttccg cctgaagcat tcgacgggct ttctcgtgat
      841 tcccaccata atccagagcg gctacaatcg cagtggtcat gacttccgca tccgtcatct
      901 ggcattgtgg atcatctcga tgatgaaggg ctttcaacat atcgtcacaa aggcaataaa
      961 ctgctacaat tcgggtgtcc atgtgttcct ccggtttgtt gtggtttggg taccatcaac
     1021 tttaccagat acacatggac ttttttcatc aatcacccaa ggtggcaact tgggttaagt
     1081 atgatacact ctttggaacg tgacaaaagc ctctgagaga tggacgctaa aaccctgcaa
     1141 acccttgaat atcccaagat cctcgatcgg ctagcgagtc attgcgcgtt tcccgcctca
     1201 gtggataaag cccaccaatt acttcccaca aaagacattg aagaggcaag gcaccgacag
     1261 gcatccacac gcgaagcact gagcttgctc gccacgcacc ccgatctgag cattggaggg
     1321 gcgcgcgatg tgcgccctgc cgttgatctg gcgaaccacg gggggatcct tctacccaat
     1381 gacctgctcg acatcaaatc gactttgatt gccgctcgca ccttggagcg tttgttcgag
     1441 cgactaggag aacaataccc ccacataaca gagatcgccc tgcgcatgtc tccaccatca
     1501 ggcatgatcg atagcatctc gcgcgctatc tccgagcgtg gcgaaatact agacactgcc
     1561 tccgagaaat tggcaaatat ccgacgtgac ctgcgcgttg tacacgaccg cctgctggca
     1621 aagctgcaac ggatggtggg cgatccgcat aatgcccagt tcttgcaaga atccctcatt
     1681 acacaaagag atggtcgcta cgtcctgcca ctcaggtcgg aatttaaagg tcggatcaag
     1741 tccatcgtcc acgatcaatc tgc
//
LOCUS       DBNHOH010000142         9672 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1675050, whole genome shotgun sequence.
ACCESSION   DBNHOH010000142 DBNHOH010000000
VERSION     DBNHOH010000142.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9672)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9672)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9672
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1675050"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="AC16V7_02590"
     CDS             <1..554
                     /locus_tag="AC16V7_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HIR6256035.1"
                     /translation="QSATKFIGGHSDLLAGVATTRDKALWQALRKSRELSGATPGTLE
                     AYLAVRGARTLALRLQRAQQTAMILAERLEKHRLVTRVRYPGLPSHSTHAIAKRVLKG
                     FGTMISFDVLGGAEVADAVCRNVRLICHATSLGSVETTMERRAAIPGQEYLPPSLLRL
                     SVGIEDVDDLWYDLDSAIRSAAS"
     gene            910..2169
                     /locus_tag="AC16V7_02595"
     CDS             910..2169
                     /locus_tag="AC16V7_02595"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIR6256036.1"
                     /translation="MIKPTVKKVVLAYSGGLDTSVIVPWLKENYGCEVVCFTADIGQV
                     EELDGLEAKALASGASQLIIHDMREEFASEYIFPVLLAGAVYERKYLLGTSFARPLIA
                     KHQVEVAHEVGADAVAHGATGKGNDQVRFELTYTALDPRLKVIAPWREWDIRSREDAI
                     AYASAHNIPVTATVKSIYSRDRNLWHLSHEGGPLEDPWREPDEDMFQLSVSPEQAPEQ
                     PEYVEITFDSGTPVAVNGQKMTPAAMVSKLNKLGGAHGIGRADLVESRLVGMKSHGVY
                     ETPGGAILLAAHRELESLVLDRDTIQYKDMVALKYAELVYNGMWFSPLREALAAFVDS
                     TQGPVTGVVRLKLYKGNIIPAGRKSPFSMYREDFATFGQEDVYDQSDAEGFIHLYGLP
                     LKVRAMNGLPISGLNMPKPDYSRFKRD"
     gene            2225..3598
                     /gene="argH"
                     /locus_tag="AC16V7_02600"
     CDS             2225..3598
                     /gene="argH"
                     /locus_tag="AC16V7_02600"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR6256037.1"
                     /translation="MTLWGGRFRGKLDPNAWSLNASLRIDQRLAPQDVRGSLAWAKAL
                     EIAGVLTMGESAQIQHGLERIGEELHSGVFTFLESDEDIHTAVERRLGELIGPLAGKL
                     HTGRSRNDQVVTDFRLWLLENLPALGTAIANLQAALLQRAESDLGTLMPGYTHLQRAQ
                     PILLSHWWLSHFWPLARDRERLQDLAGRTAVLPLGAGALAGAPFPVDRIDLAQELGFD
                     HPAPNSIDAVSDRDFAAEFLFCAALMGVHLSRMAEGVILFSSAEYGFFELSDAFATGS
                     SLMPQKKNPDVFELARGKTGSLIGWLSGLMATLKGLPSAYDKDLQEDKAPVFSAYDTL
                     MSLLPVMGGALRTLTIHPERLRSAIDAGMLATDLADYLVAKGIPFRQAHELTGKAVQQ
                     AAAQGKSLDALTLEEYQSLSPIFEAGVFAVFDPQYSVRRRNIYGGTAPESVAVQILEA
                     HNFLEKS"
     gene            3717..4427
                     /gene="lipB"
                     /locus_tag="AC16V7_02605"
     CDS             3717..4427
                     /gene="lipB"
                     /locus_tag="AC16V7_02605"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HIR6256038.1"
                     /translation="MSNRCHVHRLGLMDYQQAWQLQDSLAAEIAEESRPDSLLLLEHP
                     HTYTFGRRGNAENLLWDEIELARRGVSIHWVDRGGDVTYHGPGQLVGYPLLKLGSAKL
                     RVDASNTSAHLPQADYVGYVRRLEQVIILTLARLGVSARQVEGLTGVWVDTQDSPAKI
                     AAIGVKVDARGVTRHGFALNVDPDMSYWEGIIGCGLVGYPVTCMANLLAEHPPMAQVI
                     DETILAFAQVFGYEMIEV"
     gene            4606..5244
                     /locus_tag="AC16V7_02610"
     CDS             4606..5244
                     /locus_tag="AC16V7_02610"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR6256039.1"
                     /translation="MASQSYQLIMRTGPTPGKVFELDKSEIYLGRDVSNDIVVNDSEV
                     SRKHARMIMQAGGYILEDLGSTNGTFVGGQRLMGPHGLRPGEVVMLGENVTLAFEVGY
                     DPDATMVAAPAQMPARPPSQERQAQVPRAQQPAYTGQIPQGPAEPYYEGEVDEKKTNT
                     RTLILAGCGVLFIALCACIAGLFFIDQMRMWCEVPFKWFSFLYPLIGGAPCP"
     gene            5362..5844
                     /locus_tag="AC16V7_02615"
     CDS             5362..5844
                     /locus_tag="AC16V7_02615"
                     /inference="COORDINATES: protein motif:HMM:NF023295.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR6256040.1"
                     /translation="MASTIDREAAVALMMEKYETCQRLFYLPSEAGQHCVYALLCENG
                     SLYIGQTSDLMKRFREHQAGRAADWTRRHKPLRIVHIETVDTRQEAMRLEYRWKTSSG
                     RKRLKRLAKAGNERLIKAVTELSQAFTLALPHPKALEIQDEMDFFRALSAAIQGDEPE
                     "
     gene            5886..9026
                     /locus_tag="AC16V7_02620"
     CDS             5886..9026
                     /locus_tag="AC16V7_02620"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6256041.1"
                     /translation="MNERQKLEQAIAALEAQRNVLGDAVVDPAVAGLRKQLAELEPPS
                     DQQLKLATLLYTDIVDSTHLIEGLDPEDIIHIMDGALKYLSTVIDQHGGHVTRFMGDG
                     FKAVFGTPVAREDDAEQAVRAGLGLLKAAEVYAAKIEQEWGRSGFNIRVGINTGPVAI
                     GGLSEAGDTIMGLTVNVAARLENAAPAGGVLISHNTYQHIRGVFDVQALEPLHAKSIA
                     EPILVYLVQRIKARAFYRESRGVEGIETRMVGRDAELRHLQDALYSAIEKRQVHRVTV
                     IGEAGLGKTRLLYEFENWFDLLPEEFWVFKGRAFRYTQSLPFALARDIFSLRFQIQDS
                     DSSAVAKQKLEAGFVDFMGEAGIEKSHFIGQLLGFDFTDSPFIAGIRGDVKQIRNRAF
                     YYTTQFFSAVAAQDPVIILLEDIHWADDGSLDLVEHLSRNWRGFPLLIVGLTQPSLFE
                     QRSTWGEGDDNHSRIELHPLSKQECRRLVLEILRKVVDLPQVLEKTIIGTADGIPFYV
                     EELVRMMIEDGVIIKGEQEWRIQPDKLAKLRVPPTLIAVLQARLDRLSQTEREVLQRA
                     SVVGRIFWETTLEQMFENNQAGSITNVSELHQILQILRSREMIFRHEPSAFDGTVEFI
                     FAHTMLHEVTYESVLKRDRRAYHAQVAAWLEKQGSERVDEHAGLIAGHYALAGVANKA
                     VRWYIRAADLALRISAFGEARAMYTQALDLLSGKDQDRLPIQLHLGKVYYQLSDYSAA
                     RETLEIAQRIAQDLDDRPRSADALYWLSQVASAGGDYQQAQSYLEESLPLARVAGDRA
                     TLAQVLYGLGDLQWRMGNFDQARLNCEESLVLARQLGDLNLELYALNRLGTVPLVKGE
                     LDKAQPFFEETLERARQAGNRDREANALNNLGAIAYERGDLVATQAYFQNALSLVREI
                     GQQEFEAVLLSNLADISIRLDKLPAARKYICEALDIALAIGAIPGVLFSIKNIGWLLT
                     KQGQTERGLALLGMVLYHPAAFSENIREVHNALEHLGLVADEPVVAAGLELGKDLALD
                     LVAAELLMEMRCAKEY"
     gene            complement(9133..>9672)
                     /locus_tag="AC16V7_02625"
     CDS             complement(9133..>9672)
                     /locus_tag="AC16V7_02625"
                     /inference="COORDINATES: protein motif:HMM:NF025871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HIR6256042.1"
                     /translation="HVASFPGYLKAAIDAADDDGVRQELERNLADEIANPKPHPELWL
                     DFAAGLGLDPANIANGAARPAAIQMVNTFDRLAQGDSAAALAALYAYESQQPEVSRQK
                     IEGLRLRYGVDSLDALAYFEVHAEADIQHRDGERQALLRCLENGVSAEVVLESAEQAL
                     DAYWGLLDGICVEAGITTG"
BASE COUNT         2275 a         2607 c         2691 g         2099 t
ORIGIN      
        1 tccagtcggc aacaaagttc attggtggtc actctgatct actggcaggg gtcgccacga
       61 caagggacaa agcattgtgg caggcactca ggaaatcccg tgagctaagc ggtgcaacac
      121 ccggaacgct cgaggcatac cttgccgtcc gcggcgccag gacgctcgcc cttcgtctgc
      181 aacgggcaca gcaaacagca atgattctgg cggagcgact tgagaagcat cggctggtca
      241 ctcgtgtgag gtatccgggc ctgccctcgc attcgacgca tgctatcgcc aagcgtgtgc
      301 ttaaaggttt cggcactatg atctcattcg atgtacttgg aggtgcagag gtcgccgacg
      361 ccgtatgtcg aaacgtccgg ctcatctgcc acgccaccag tctcggttca gtagaaacaa
      421 ctatggaacg aagagccgcg atccccggac aggaatacct cccaccctcg ctcctaagac
      481 tcagcgtggg tatcgaggac gtggatgatc tctggtatga tctcgactca gcgatacgct
      541 ctgccgcatc ttgagcaggt gcgtcccaac ccgttgatac aacagactcg ctccgctcgc
      601 tgctgaactc aaacactact ttttcaacag cctgaattta ttttggtagt catattcaga
      661 aattaacctt gacgcggatt ctatcgggtg ataaactgcc ccccaatatg caagaaacca
      721 atgtttttag aacacgcgcc cgtcgtacct ccaccccatg ccgggaaagg tgggtgtctg
      781 cgcgtgcaaa agtgtcatag tttcttcttt tcactgatca aaaccctcct tcccaggagg
      841 gtttttttat ccaaaacgcg ggtttcggcc taacccgtct cccacgaaaa tcttagaacg
      901 gaggttgtca tgataaaacc aactgtcaag aaagtggtcc ttgcttattc cggtggcctg
      961 gacacatcgg tgatagtgcc ctggctcaag gaaaactacg gctgcgaagt ggtgtgcttc
     1021 accgcagata tcggtcaggt cgaagagctg gatgggctgg aggcgaaggc gcttgccagc
     1081 ggagccagcc agcttatcat ccacgacatg cgcgaggaat ttgcatccga gtatatcttc
     1141 ccggtgctgc tcgccggcgc agtatatgag cgcaagtacc tgctgggcac gtccttcgcc
     1201 cggcctctca tcgccaagca ccaggtggag gtggcccatg aagtgggagc agatgccgta
     1261 gctcacggcg caaccggaaa aggcaacgat caggtgcgtt tcgagctcac ctacactgcc
     1321 cttgatcccc gcctgaaggt gatcgcaccc tggcgcgagt gggatatccg ctcgcgagag
     1381 gatgccatcg cctacgccag cgcccataat atccccgtga ctgccacggt caagtcgatc
     1441 tacagccgag atcgcaactt atggcacctt tcacacgaag gcggcccgct ggaagatccc
     1501 tggcgggagc cggatgaaga catgttccag ctcagcgtct cgccggagca agccccggaa
     1561 cagcccgagt atgttgagat tacgttcgat agcggcactc cggtagcagt gaacgggcaa
     1621 aagatgaccc ctgccgccat ggtgagcaaa ttgaacaaac tggggggagc acacggcatc
     1681 ggccgcgctg acctggtgga aagccgcctg gtggggatga aatcccatgg tgtttatgaa
     1741 acacccggtg gtgccatcct gctggcagct caccgcgagc ttgaatcgct ggtgctggac
     1801 agagatacca tccagtacaa ggacatggta gcattgaaat acgccgaact ggtatacaac
     1861 ggcatgtggt tctcaccttt gcgcgaggct ctggcagcct tcgtggatag cacgcaggga
     1921 ccggtcaccg gcgtggtgcg cctgaagctg tataaaggca acatcatccc ggctggacga
     1981 aagagtccct tcagcatgta ccgagaggac tttgccacct ttgggcaaga ggatgtctac
     2041 gaccaatccg acgcagaagg cttcatccat ctatacggct tacccctgaa agtgcgtgcc
     2101 atgaatggat tacccatctc aggactcaat atgcccaaac cggattactc gagattcaag
     2161 cgagactaga tttagccact tatccaatca ccatgtaatc ctgttaagga agagagagaa
     2221 tatcatgaca ttatggggag gtcgttttcg aggtaagctc gatcctaacg cctggtctct
     2281 gaacgcatcg ctacgcattg accagcgcct tgcaccccaa gatgtacgcg gcagcctagc
     2341 ttgggcaaaa gcgcttgaaa tagccggcgt attgacaatg ggcgagagcg cacagatcca
     2401 acatggctta gaaagaattg gcgaagagct gcatagcggc gtgtttacct tcctggagag
     2461 tgatgaggat atccataccg cggtagaacg ccggttgggt gagttgatcg gcccactggc
     2521 ggggaagctg cacaccggtc gcagccgtaa cgatcaagtg gtgaccgatt ttcgtctgtg
     2581 gctgctggag aacctgcccg ccttggggac tgccatagcc aacctgcaag cggcgttgct
     2641 tcagcgcgcg gaatccgatt tgggcaccct gatgccaggc tacacccacc tgcagcgcgc
     2701 ccagccgatc ctgctcagcc actggtggct atctcacttt tggccgctgg ctcgcgaccg
     2761 ggaacgcctg caagacctgg caggacgcac cgctgtgctc cccctgggag caggggcgct
     2821 ggctggagcg ccattcccag tagaccgcat cgacttagca caagagctgg gcttcgatca
     2881 tcccgcccct aatagcatcg acgctgtctc tgaccgcgac ttcgctgccg agtttttatt
     2941 ctgcgcagca ttgatgggcg tgcacctcag ccggatggcg gaaggcgtaa tcctgttctc
     3001 ctcagcggag tacggctttt tcgagctatc cgatgcgttt gccaccggct ccagcctgat
     3061 gccgcagaaa aagaacccgg acgtgtttga actggcgcgc ggcaagacag gcagcctgat
     3121 aggctggctg agcggactga tggcaaccct caaaggcctg ccatctgcct acgacaagga
     3181 cttgcaggaa gacaaagcgc ctgtcttcag cgcttacgac accctgatgt ctctactgcc
     3241 ggtgatggga ggcgccctgc gcacgcttac catccatcct gagcgcctgc gctccgccat
     3301 cgatgccggt atgctggcaa ccgatctggc agattacttg gtagcgaaag gcatcccctt
     3361 ccgccaggcc cacgagttga caggcaaagc cgtgcagcag gctgccgcgc aaggcaaatc
     3421 tctggacgca ctgacgcttg aggagtacca atcgttaagt ccgatcttcg aggctggcgt
     3481 cttcgccgtc tttgatccac agtacagcgt gcgccggcga aatatttatg gcggcaccgc
     3541 tcctgaatcc gttgcggtac aaatactaga agcgcataac ttcctggaaa aatcctaaaa
     3601 agatgtttgc agacaaggca aaggtatatc cgctccaggg catatgcaat cccgcaaaac
     3661 ggggacccag gggagcaggt ccacggaaca cctttttggg ctatacttca catgctatgt
     3721 cgaatcgctg ccatgtccac cgcctgggtc tgatggatta ccagcaagcc tggcaactac
     3781 aagacagtct agccgccgag atcgccgaag agtcgcgccc agactccctg ctattgctcg
     3841 agcacccgca tacctacacg tttgggcggc gcggcaacgc ggagaacctg ctgtgggatg
     3901 aaatcgagct tgcgcgccgc ggcgtaagca tccactgggt ggaccggggc ggcgatgtta
     3961 cctatcacgg ccctgggcag ctggtgggct acccattgct aaaattgggg tctgccaagc
     4021 tgcgtgtgga tgcatcgaac acttctgcgc acctgcccca agcagattac gtcggctatg
     4081 tgcgcaggct ggagcaggtc atcatcctga ccctggcacg cctgggcgta tctgcccggc
     4141 aggtggaagg cctgaccggg gtttgggtgg atacccaaga ctcgcccgcc aaaatcgccg
     4201 ccattggggt taaggtagat gcgcgcgggg tgacccgaca tggcttcgcc ttgaatgtgg
     4261 atccagacat gagctattgg gaagggatca ttggctgcgg gctggtcggc tacccggtca
     4321 cctgcatggc gaatctgctc gccgaacacc ctccgatggc acaggtgatt gatgagacca
     4381 tcctggcgtt tgctcaggta ttcggatacg agatgatcga ggtttaattc cgtttgacac
     4441 aaaccccaaa actcagtaca ctatgagcat actgaggcct tatgcagttg agggaaaaga
     4501 tctctcttat tgtgtaaatt catcatattg aaccgagcag gagagcctga tcagactgct
     4561 ttaccgatca acgggcttca agccaacctt tgcgaggagg aataaatggc ttcacaatcc
     4621 taccaattaa tcatgcgcac aggcccaaca ccgggaaaag tattcgagct cgacaagagt
     4681 gaaatctact taggtcgtga cgtgagcaac gatatcgtgg ttaacgattc ggaggtctcg
     4741 cgcaagcatg cccgcatgat catgcaagct gggggataca tactggaaga tctaggctct
     4801 accaacggca cctttgtcgg tggccagcgg ctgatgggcc cacacgggtt gcgcccaggc
     4861 gaagttgtca tgctgggcga gaacgttacc ctggcctttg aagtcggcta tgaccccgac
     4921 gcaaccatgg tggctgcccc agctcaaatg ccggctcgcc cgccatccca ggagcggcaa
     4981 gcccaggttc ctcgcgcgca gcaacccgca tacaccggtc agatcccgca aggccccgct
     5041 gaaccttatt acgaaggcga ggttgatgag aaaaaaacca atactcgcac cctgatcctc
     5101 gccggctgcg gcgtgctgtt catcgcgctg tgcgcatgca tcgcaggctt gttttttatc
     5161 gaccagatga gaatgtggtg cgaggtgccc ttcaagtggt tcagcttcct ctatccgctg
     5221 atcggtgggg cgccctgccc ataagaattt cacaccatct ccagaacaac cattatcctt
     5281 gcctctatcc tatacatcca gggtagttgg atgcattcat tgggcatggg gacacccgcc
     5341 tcgtaaaggc attgattaag catggcatcc accattgacc gggaagcagc cgtcgctttg
     5401 atgatggaga agtacgaaac ctgccaaagg ctgttctact tgccgtcaga ggcaggccag
     5461 cattgcgttt atgcccttct ttgtgaaaat ggcagcctgt acatcggcca gacttccgac
     5521 ctgatgaaac gtttccgaga gcaccaggct ggccgggcgg ctgattggac gcggcggcac
     5581 aagccactcc gcatcgttca cattgagacg gtggatacac gccaggaagc catgcgcctg
     5641 gaatatcgtt ggaagacaag ctctggaaga aagcggctca aacgactggc aaaagctggc
     5701 aatgagcgct tgattaaggc tgtgacggaa ctctcgcaag cctttaccct ggcgctaccc
     5761 caccccaaag cgctggagat ccaagacgag atggatttct ttcgtgcctt atccgctgcc
     5821 atccaggggg atgagccgga ataaaattat cttgaaaatc gccgggaaat taccaagaga
     5881 agtagatgaa cgaacgccaa aaactagaac aagccattgc tgctttagaa gcacagcgaa
     5941 acgtgctcgg agacgccgtg gtcgatcctg cggttgctgg cctgcgcaag caattagccg
     6001 agcttgaacc tccctcagat cagcaactca agctggctac cctgctttat acagatatcg
     6061 tcgactcaac ccatctcatt gaaggattag atcctgaaga catcatccac atcatggatg
     6121 gggcactaaa gtacctgtca accgtcattg atcagcatgg tggacatgta acccgcttca
     6181 tgggtgatgg tttcaaggct gtctttggca cccccgtcgc tcgagaagat gatgctgaac
     6241 aagctgttcg agccgggttg ggtttgctca aagcagctga ggtttatgcc gccaaaattg
     6301 agcaggaatg gggtcgtagt ggatttaata tccgagttgg aattaacacg ggtccggtag
     6361 caattggggg cctttctgag gcaggagata ccatcatggg cttgacggtc aacgtggcag
     6421 cccgcttgga aaatgctgct cctgctggtg gtgtcttgat atctcacaac acctatcaac
     6481 atatccgtgg cgtttttgat gtgcaagccc ttgagccttt acatgccaaa agtatagccg
     6541 aaccaatact ggtctacctg gtacagagga ttaaagctcg cgccttctac cgagaatccc
     6601 ggggggttga gggtattgaa acacgcatgg tcgggcgaga tgcagaattg agacatttac
     6661 aagatgccct atactccgct atcgagaaac gccaggtgca tagggtcaca gtgattggtg
     6721 aagctggatt gggcaaaact cgcctgttgt acgagtttga aaactggttc gacttgcttc
     6781 cggaggagtt ttgggtattt aagggaaggg ccttccggta tacacaaagt ttaccctttg
     6841 ctttggcgcg cgatattttt tcattgcgtt tccagattca agacagcgac tcttccgcgg
     6901 tcgccaagca gaagctagaa gcaggtttcg ttgactttat gggcgaggcg ggaatcgaga
     6961 aatcccactt catcgggcag ttactgggct tcgactttac ggatagtcct ttcatcgccg
     7021 gcatccgagg tgatgtcaaa caaattcgca accgtgcctt ctattacacc acccagttct
     7081 tttctgcagt cgcagcgcag gacccagtga taattctctt agaagatatc cactgggcgg
     7141 atgatggttc attggatctg gtagagcatc tgtccaggaa ttggcgggga tttcctttgc
     7201 tgattgttgg cctgacccag ccatctttgt ttgaacaacg gtctacctgg ggagaagggg
     7261 acgacaatca cagtcgcatt gaactgcacc ctttatcgaa acaagaatgc cgccgcttgg
     7321 tcttggaaat cctgcggaaa gtggttgatc ttccacaggt gctggagaaa acaataatcg
     7381 gcaccgccga tggcatccca ttctacgttg aagagttggt gaggatgatg attgaggatg
     7441 gggtcattat caaaggggag caagaatggc gaatccagcc cgataagttg gccaagttgc
     7501 gagtgccccc aacgttgatc gccgtcttgc aagcaaggct tgatcgatta tcccaaaccg
     7561 agcgcgaagt tctacaacgc gcctccgtag tggggcgtat cttctgggag acgaccttag
     7621 agcaaatgtt tgagaataat caggcgggat cgataaccaa cgtctcggag cttcaccaga
     7681 tcctgcaaat cttgcgtagc cgcgagatga tcttccggca tgagccttca gcctttgacg
     7741 gaacggtgga attcatcttc gcgcacacca tgctccacga ggtaacttat gaaagtgtat
     7801 taaagcgcga tcgacgggct taccacgccc aggtagctgc ctggttggag aaacagggca
     7861 gtgagcgggt agatgagcat gctggtttga tcgctgggca ttacgcgctg gcaggggtag
     7921 caaataaggc agtcagatgg tacatccgag cggctgatct cgcgctaaga atcagcgcct
     7981 tcggagaagc tcgggcgatg tacacacagg ccttggattt gttgtcggga aaagatcaag
     8041 atcgattgcc gattcaactt catctgggga aagtgtatta ccagttgagt gattattccg
     8101 ccgctaggga aacactggaa attgcacaga gaatcgccca agatttagat gaccggccgc
     8161 gcagtgcgga tgccttatac tggctcagcc aagtcgcctc tgctggaggc gattatcaac
     8221 aagcgcaatc ctacttagag gaaagcctgc ccctggcgcg ggttgccggt gatagggcta
     8281 cattagcgca agttttgtac ggattagggg atttacaatg gcgtatgggc aactttgatc
     8341 aggcgcgtct caactgtgaa gaaagcctgg ttctagcccg tcaactgggt gatctaaacc
     8401 tggaattata cgcccttaac cgattgggga cggtgcctct cgttaaagga gagctggata
     8461 aagcacagcc attctttgaa gagaccctgg agcgagcgcg acaagcaggc aaccgggacc
     8521 gggaggccaa cgctctcaac aacctgggtg caatcgccta tgaacgtggc gacctggtgg
     8581 caacccaggc ttatttccag aatgcacttt cgctcgtccg ggagattggg cagcaggaat
     8641 ttgaagccgt attgctcagc aatttggcag acatatcgat ccgtttagac aaattacctg
     8701 ctgcaaggaa gtacatatgt gaagcattgg atatagccct agctattgga gcaattccag
     8761 gggttttgtt ttcaataaaa aatattggct ggttactaac caaacaagga caaactgaac
     8821 gaggattagc tctattgggg atggtcttgt atcatcctgc tgcatttagc gaaaacataa
     8881 gggaggtaca caatgctctt gaacacctcg ggcttgtagc ggacgaacca gttgtggcag
     8941 caggtcttga actcgggaaa gatctcgctt tggatcttgt cgcagcggaa ctgcttatgg
     9001 aaatgaggtg cgcaaaagaa tattgatgcc ccttcagaac aggaaaccgt cacattcccc
     9061 ctagagatag ggggattttt gggtaaaacc cggaaaatcc cccacatcat ccatgcgagg
     9121 gtagtcgcag gcctaacctg tggttatccc cgcctccacg caaatgccat ccagtaatcc
     9181 ccaataagca tccaaggcct gctcggctga ctccaggaca acctcggctg agacgccgtt
     9241 ttccaggcaa cgtagaagcg cctggcgctc gccatcccgg tgctggatgt cggcttcggc
     9301 gtgcacttcg aaataggcca gcgcatcgag actgtccaca ccataacgca agcgcaatcc
     9361 ttcgatcttc tgccgcgaga cttcgggctg ctggctttcg taggcatata aggctgccaa
     9421 tgccgccgca ctatcaccct gggcaagccg gtcgaaggtg ttcaccattt ggattgcggc
     9481 aggtcgcgcc gctccgttgg caatattggc agggtccagg cccaagcctg cggcgaaatc
     9541 cagccaaagt tccggatggg gtttcggatt ggcgatctca tccgccaggt tgcgctcaag
     9601 ctcctggcgc acgccgtcat catcggcggc atcgatggcc gctttgagat agccggggaa
     9661 ggatgccacg tg
//
LOCUS       DBNHOH010000143         1814 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1290224, whole genome shotgun sequence.
ACCESSION   DBNHOH010000143 DBNHOH010000000
VERSION     DBNHOH010000143.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1290224"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1814)
                     /locus_tag="AC16V7_02630"
     CDS             complement(<1..>1814)
                     /locus_tag="AC16V7_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256043.1"
                     /translation="GSQGVDPVFGHGRIDAKQAVTKVASDYSPPGGLASNSDDSSAYA
                     HARKMVRDGTGKLHLIWHTADSSLFRIRYATSVDNGDSWIQQGDVFTSALETYHPALA
                     TDNNYLYVAIPSRKGENQPYQILFTRKPVSGGSWSQAEPLMGGTYNAVRPHLYLDPTN
                     GRLHLIASSYDDSTSLYYRATNNPAVPEPASPSWEQLSQFNPSNTADNLTRYAVLHAN
                     GDKIYVVARTVQNFFIFTYLNMFTVHSTNGGQTWTDQTHISAHLAMTSGEYGISLAGV
                     GDRIYMGYEVGTTLYFRRNNGNGWSDYMTLEPGDANNVYKWPTITQADDGQAWMLFEV
                     NKQLYMRHYDGSIWQPKEAIGPGTYANLGLGTGNGQVEWVSTNCNGSPFELVYDSRSV
                     GSNPLPPTPTSTPTSTITPTPTATPTSTQTDTPTPTPTTTPTSTSTSTNTPTATNTPT
                     STATQTPTLSPPVTVVDALTSGEIFVAGTVSGDYTSTQVKGGHVETLTEVVSGGKPQN
                     RYSYLEHKWTFNVTSGNTVTLYAYTWKGECIPTPENSCDGDTFKFAYSTDDANYFDMF
                     TVSSTSLDNIEAYSLPSSTEGTVYVRVTDSNHESGNRTL"
BASE COUNT          384 a          424 c          561 g          445 t
ORIGIN      
        1 agcgtccggt ttcctgattc gtgattgctg tcggtgacac gcacgtagac ggtgccctca
       61 gtggaagagg gcaagctgta ggcttcaata ttatcgagag aggtgctgct cacagtgaac
      121 atgtcgaaat agttggcatc atctgtcgag taagcaaact tgaaggtgtc accatcgcag
      181 gagttttctg gggtgggaat acattcaccc ttccaggtat aagcatacaa ggtgacggta
      241 ttgccagacg tgacattaaa ggtccatttg tgctccagat agctatagcg gttttgcggt
      301 ttgccacccg atacaacttc agtgagtgtc tccacgtggc cacctttgac ctgggtagag
      361 gtgtagtcac cgctcacggt tccagcgaca aaaatctctc cactggtaag cgcatcgacg
      421 accgtcactg gtggtgagag cgtgggggtt tgtgtagctg ttgaggtagg ggtattggtg
      481 gcagtgggcg tgtttgtaga tgtcgacgtg ctggtaggtg ttgttgttgg ggtgggtgtg
      541 ggggtatccg tctgcgtact ggtaggtgta gcggtgggag tgggcgtgat tgtagaggtt
      601 ggcgtactag tgggtgttgg aggaagtgga tttgaaccga cgctgcgcga atcgtacacc
      661 agctcgaaag gtgagccgtt gcagtttgta gatacccatt ccacctggcc atttccagtg
      721 ccgagcccca ggttggcata ggtgcccggg ccgatcgcct ctttaggctg ccagatggag
      781 ccatcgtaat ggcgcatgta caattgcttg ttgacctcga acagcatcca ggcctgccca
      841 tcatccgcct gggtgatggt gggccatttg taaacattgt tcgcatcgcc tggttccagc
      901 gtcatgtaat cactccagcc gttgccatta ttgcgacgga agtacagggt agttcctacc
      961 tcatagccca tatagatgcg atccccgacc cctgccaggg aaatgccgta ttcacccgag
     1021 gtcatggcca ggtgggccga gatatgggtc tggtcggtcc aggtttgacc accgttggtc
     1081 gagtggacgg tgaacatatt caagtaggtg aagatgaaga aattttgaac cgtccgcgcc
     1141 acaacataga ttttgtcccc attggcgtga aggactgcat agcgcgttaa gttatcagcg
     1201 gtattggagg ggttgaactg gctgagttgc tcccaggagg gactggccgg ttctggtacc
     1261 gcggggttat tcgtggcacg gtaataaagg gaagtagaat catcatagct ggaggcgatc
     1321 aggtgcagcc tgccgttggt ggggtcgagg taaagatgcg ggcgcaccgc gttatacgtg
     1381 cctcccatga gaggctcagc ttgagaccag ctacctcccg atacgggttt acgcgtaaac
     1441 agaatctggt agggttgatt ttcacccttg cggctgggga tggccacata taggtaatta
     1501 ttatcggtcg ccagggccgg atggtaggtc tcaagcgcgc tggtgaagac atccccttgc
     1561 tgtatccagc tgtcgccgtt atcgacagaa gttgcatagc gtatcctgaa aagcgaacta
     1621 tcagccgtgt gccagatcaa gtgcagcttt cccgtgccgt cgcgcaccat tttgcgggca
     1681 tgggcataag cagatgaatc atcgctgttt gaagcaagcc caccgggcgg gctgtaatca
     1741 gaagcaactt tagtcactgc ctgtttagcg tcgatgcgcc cgtgaccgaa tacgggatcg
     1801 acaccttgcg aacc
//
LOCUS       DBNHOH010000144         4583 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_8583, whole genome shotgun sequence.
ACCESSION   DBNHOH010000144 DBNHOH010000000
VERSION     DBNHOH010000144.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_8583"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..3472)
                     /locus_tag="AC16V7_02635"
     CDS             complement(149..3472)
                     /locus_tag="AC16V7_02635"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6256044.1"
                     /translation="MTFLLSLFTSPAYIDLPQSLVGRLIVGVFWVLGVALLVLFLRRW
                     RAFHRSRTTRFWGILAILAVLVPLTSLFIGLRLAARGALPPPGIPIDRLGPAMMVFIA
                     VPWTLAGGFLGIVPAAALAGFSGLLLGLWDTHNPFTPLEFAFLGILFSAAINQRYRTR
                     SYVLLRHPLFTSLLIAAFYPLIALVSASLIADGPLAIRLDYAIARLGAASLSVGIPLL
                     VAGLFSEVVAASFPGAWGRIGLLLPSPAEKSLQARFLYGTAPLAFILTVLLMVGDWVV
                     AGAAARQILRDRMADAARLASEGVPFFLEAGQNLIAQLSSDPRLYTSDPDDLIGVLAQ
                     DLRSVPFFTQLFILDEVGNPLAGYPIENYASAQPPPEEQMGIQLSLAGVPYQIYTVPP
                     DSDDKASKVSFVAAIRDENESVRGVLIGRTELDVNPFTKPIIASLSSLAGSDGEGILL
                     DEDGRILYHSNPALLMAVYTGRTAEGPEFYNDTAPDGTRRLVYYQPALGRPWAVVLTV
                     PARRTQQIALNIAGPLLGMIVLLAIFSVLLLRFGLRVVTASLKRLAVQAGVIAQGQLD
                     TPISFNGEDEVGQLGRAFEQMRLSLKARLDELNRLLLVSQGVASSLEMDEAVKPLLEA
                     SLSSGASSARVVLAPETVPDLEGSPVSPTVYAIGSAGDTYAYLDDQILELTRNQERVV
                     MHSLARPRLINFKADDLRPQALMALPLRHENQYYGALWTAYDQSHKFGEEEIRFQVTL
                     AGQAALAAANARLYMNAEIGRQRLAAILASTPDPVLVTDQQNRLLLSNPAAWRALGLG
                     VEWEEGQPIERAVSEIPLLELIHSSDDKRSVEVTLPDGRVYYATASSVIADGIRVGRV
                     CILRDITYFKELDSLKSEFVSTVSHDLRSPLTLMRGYATMLEMVGELNEQQAGYVRKI
                     VTSVESMTKLVGNLLDLGRIEAGIDLQLEMIHVQDVIERVIGALQLQATQKRVQLVNE
                     VVRQVVPVIEADQALLQQALHNLVDNAIKYTESGGKVSVRVRTKVGVASEPDYLVIEV
                     QDNGIGIAPVDQPRLFEKFFRGAQEGGKKQGGSGLGLAIVKSIAERHGGKVWMESQIG
                     RGSTFYLSIPFRQRKGDLT"
     gene            complement(3582..4310)
                     /locus_tag="AC16V7_02640"
     CDS             complement(3582..4310)
                     /locus_tag="AC16V7_02640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR6256045.1"
                     /translation="MSERSTSLYSSKYRHTDWDRRRRVLRWLLKYIGFTLLAKLDRVE
                     GLENVPAQGPAILMINHIAFIDPIVVLHVMPRNIIPLAKIEVYNYPLVGIFPRLWGVI
                     PVRREEVDRRAVQQVLEVLRAGEMVLVAPEGTRASHLSRGKEGIAYLSSHSGTPIVPV
                     AIEGTEGFPSLRFFSLAWKTDGAVVRFGRPFRFRPDLKRPDREQLRQMSDEAMYILAA
                     MLPERRRGYYADLSQATQETIEWV"
     gene            complement(4324..>4583)
                     /locus_tag="AC16V7_02645"
     CDS             complement(4324..>4583)
                     /locus_tag="AC16V7_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
                     /protein_id="HIR6256046.1"
                     /translation="FWVANFIAAMAFGAAHLGSAMVILGGTSLDVISPLVMGEIFLIN
                     GIVGMVAGERYVQQGLVAAAGIHFWMDIVWHVVWPVFLSSL"
BASE COUNT         1205 a         1328 c         1117 g          933 t
ORIGIN      
        1 acaaagacaa gcgcattgag cacctccgtc tcaagcgctt atcacgcgcg ctacctcatt
       61 gatttatcat ctgaatacat tataccaacc atgatcacgg ttggtcaaga ggaaaattac
      121 gcaaatttca cttgaatcaa aaaatcaatt acgttaaatc ccctttccgc tgtctgaatg
      181 ggatggataa atagaaggta cttcccctgc ctatttggct ctccatccac actttgccac
      241 cgtggcgttc agcaatcgac ttaacgatgg ctaaaccaag accagatcct ccttgtttct
      301 ttccaccctc ttgcgcgccc cgaaagaact tctcgaataa tcgtggctga tccactggtg
      361 ctatgccaat gccgttatcc tgcacctcaa tcaccaggta atctggttcg ctggcgaccc
      421 ccactttggt ccgcacgcgc acactcacct tcccaccgct ctccgtatat ttaatcgcgt
      481 tatccaccag attatgtaaa gcttgctgca gcaatgcctg gtcagcctca ataaccggaa
      541 ctacttgccg cacaacctca ttaaccagct gcacacgctt ctgagtggct tgcagttgaa
      601 gggcgccaat gacgcgttcg atcacatcct gaacatggat catctctaat tgaagatcta
      661 tgcccgcttc tatgcgtcct aggtcgagca ggttgccaac cagcttggtc atcgactcca
      721 cgctagttac aattttacgc acataaccag cctgttgttc gtttagctca cctaccatct
      781 ccaacatggt ggcgtagccg cgcatcaacg tcagcgggga gcgcagatca tgactcaccg
      841 tagagacaaa ttccgatttg agggaatcca actctttgaa ataggtgatg tctcgcaaaa
      901 tgcacacccg tcccacacgg attccatcag caataaccga tgaagctgtg gcgtagtaca
      961 cgcgcccatc gggtaaggtg acctccacag aacgcttatc gtcagacgag tggatgagtt
     1021 ccagcaaggg gatctcagaa actgcgcgtt caatcggctg accctcctcc cattccacgc
     1081 ccagtcctaa ggctcgccag gctgccggat tggagagcaa caagcgattt tgctgatcag
     1141 tcacaagcac cgggtctggg gtagaagcca agatagctgc caagcgctgc ctgccaatct
     1201 ctgcattcat atacaagcgg gcattggcag ccgcaagagc ggcttgtccg gctagagtaa
     1261 cctggaaacg tatctcttcc tccccgaatt tatgggattg gtcataggca gtccaaagag
     1321 cgccgtaata ctggttttcg tggcgcaggg gtaaggccat caaagcttgt ggtcgcaagt
     1381 cgtccgcctt gaaattgatc aaacgcgggc gagccaggct gtgcattacc acacgctcct
     1441 gattgcgcgt caactccaag atctgatcat cgagataagc atacgtatcc ccggcagaac
     1501 cgattgcgta aacagttgga gaaaccgggc taccctccag atcgggtacc gtctcgggag
     1561 ccaaaaccac cctggccgag ctcgcccccg agctcagaga agcttcaagc aaaggcttga
     1621 ccgcctcatc catctctaga ctagaagcta ctccctggct gacgagcaat agcctgttta
     1681 attcatctaa tctggctttc aagctgaggc gcatctgctc aaaagcgcgg cctaattgcc
     1741 caacttcatc ttcaccattg aagctgatcg gggtgtctaa ttgaccctgt gcgattacac
     1801 cagcctgcac cgccagcctc tttagggaag cggtcaccac gcgcaaacca aaacgaagca
     1861 gcaagacgga gaatatcgct agcaggacaa tcatacccag caaggggcca gcgatattga
     1921 gcgcgatctg ctgggtacgc cgcgcgggga cggtcagcac cactgcccaa ggacgcccca
     1981 aagcaggttg gtaatagact agacggcgag tgccatctgg agcggtatcg ttgtaaaatt
     2041 ctggtccctc agccgtccgc ccggtataga ccgccatcaa caaggctggg ttcgagtggt
     2101 ataagattcg cccatcttca tccagcaaga tgccctcgcc atctgagcca gccaggctgc
     2161 tcaggctggc aataatgggt ttggtaaagg gattgacgtc tagctctgtg cgcccaatca
     2221 agacaccccg cacactctca ttttcgtcgc ggattgcagc aacaaacgag accttggagg
     2281 ctttatcatc actatcaggt ggcacagtat atatctggta aggcacacct gccagggata
     2341 gctgaatccc catttgctct tcaggagggg gttgggcact ggcatagttc tcaattggat
     2401 aaccagccag cggatttcca acttcatcaa gtataaacaa ctgggtgaag aaagggacac
     2461 tcctcaagtc ttgcgccagg acgccgatca gatcgtctgg gtcgctcgta tacaagcgcg
     2521 ggtcagaaga gagctgtgcg atcaggttct gcccggcctc taggaagaaa ggcacgcctt
     2581 ccgaagccaa acgagcagca tctgccatgc ggtcgcgcaa gatctggcgg gctgccgcgc
     2641 ccgccaccac ccaatccccc accatcaaca acactgtcag aatgaacgcc aatggagcag
     2701 taccatataa aaaccgagct tgcaggcttt tctctgctgg agaaggcaac aacagcccaa
     2761 tcctacccca ggcgcccgga aatgaagccg ctaccacctc tgagaataga cctgccacca
     2821 acagcggaat gcccactgac agagaagccg cccccaaccg ggcgatggca taatccaatc
     2881 gaatggcaag cggcccatcg gcgattaaag aggcactgac caaagcgatg agcgggtaga
     2941 aagccgcgat caacagtgac gtaaacagtg ggtggcgtag caacacatac gagcgcgtcc
     3001 tgtagcgctg gttgatagct gcactgaaaa ggattcctaa aaaggcgaat tctaagggag
     3061 taaacgggtt gtgcgtatcc cacaaaccca gcaacaagcc ggagaagccc gccaaggctg
     3121 ctgctggcac gattcccaga aatccccctg ccaaggtcca gggtaccgcg ataaagacca
     3181 tcatcgcagg accaagacga tcaatgggga tacccggagg tgggagggca cccctggcgg
     3241 caagccgcaa accgatgaat aaacttgtta gtggaactaa gacagccaaa atggctagga
     3301 tcccccaaaa acgggtggta cgggaacggt gaaaagcgcg ccagcgacgc aagaacagta
     3361 ccagcaacgc cactcccagt acccaaaaga cccctacaat caggcgtccc accagacttt
     3421 gcggaagatc aatatatgct ggggaggtga agagcgatag caggaacgtc atatatcctg
     3481 ggcggttgca attaacatgc ctggatgatt atagcatgga taccagacca ccacctctcc
     3541 aactcgttcg tggcatttag accttgtgaa tgccttgcca atcataccca ctcgatggtc
     3601 tcctgtgtag cttgagacag gtcagcataa tatccccttc gcctttctgg aagcatggct
     3661 gctagtatat acatggcttc atcactcatc tggcgtagct gctcgcggtc gggtcgcttc
     3721 agatcgggac gaaaacgaaa tggtcgtccg aaacgcacga cggctccatc ggtcttccaa
     3781 gccagcgaaa agaaacggag ggatgggaac ccctcggtgc cttcgatcgc cacaggcacg
     3841 atgggggtac ctgaatggct ggagagatag gcaatgccct ccttgccgcg agacaggtgt
     3901 gaagcccgcg tcccctcagg tgctacgagc accatctccc ccgcgcgcag cacctccaac
     3961 acttgctgca ccgcccgccg atccacttcc tcgcggcgta ctgggatcac tccccacagc
     4021 ctgggaaata tccccaccaa cgggtaattg taaacttcga ttttggctag agggataata
     4081 ttgcgcggca tgacgtgcaa tactacaatc ggatcgataa aggcaatatg gttaatcatc
     4141 aatatcgccg gaccctgggc gggcacattt tctagccctt ctacgcggtc caatttagcc
     4201 aaaagggtga agcctatgta cttgagcagc cagcgtaaaa ctcggcgacg tctatcccaa
     4261 tcagtatgcc gatatttgga tgaatagagc gatgttgatc tttcggacac agcttatccc
     4321 caatcagagc gatgaaagaa atacaggcca gacgacatgc caaacaatat ccatccaaaa
     4381 atgaatacca gctgcggcga ctaatccctg ttgcacatat cgctcgcctg ccaccatccc
     4441 gacaattcca ttaatcaaga aaatctcgcc catgacaagc ggtgagatta cgtctaagct
     4501 ggtgccgccc aatataacca tagccgaacc caaatgcgcc gcaccaaacg ccatcgcagc
     4561 gatgaaattt gcaacccaaa agg
//
LOCUS       DBNHOH010000145         2414 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_841983, whole genome shotgun sequence.
ACCESSION   DBNHOH010000145 DBNHOH010000000
VERSION     DBNHOH010000145.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_841983"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..>2414)
                     /locus_tag="AC16V7_02650"
     CDS             complement(139..>2414)
                     /locus_tag="AC16V7_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256047.1"
                     /translation="RRFLLALVAVLLLATLGSLALAWNARKQADLSTSRELAVAAINN
                     LEVDPERSILLSLQALRTVDTREAKDAMHRSLQTSRVRMALIEHTGWVRSVEFSPDVK
                     TIATTSYAWEATIWDTGSGQVLFSLPGTVARYSPDGVRLATGSLDGTLTIWNLATRNR
                     LWTKRGHTWRIFDVHFSPDGELLVSSSVDNTFIVWDTDTGQEIFSSTAKVEGFDALNN
                     VAFSPDGRLLISVDITESGNKISFWGVDRDWALLNQLPSNDKMIFSPDGKWLVSTGAG
                     IHTNAIVLWDISNANLESLDLSSLKPLVVSAAHTSMINNFAFSPDGSVLASVGQDGLA
                     KVWRPSSEGLELLMVLSGHTDQLTDVAFSPDGTRLATSSADGSVRIWDITPAGASDWF
                     TLAAHQGPIYLFDLTADGKYLATASSDGVAKVWDLASGRELLAITNHGGPLYGVAISP
                     DGTLLATAGDDNIAKIWKLRLSPGAATVELLHTLSGHIKSWLVNHKFPGLTSVAFSPD
                     GAKLATGGVDGVAKIWEVSTGQELLSIQTNPDRLGIVRLAFSPDGLLLATTNDAPYEL
                     AKIWDAASGKEISAFSGQRYIRFISGIAISPDGERAATSSANGSLKIWDAKTGEELLN
                     LVGHTGIVEGVDFSPDGKYLASAGHDCTEDVCEKPPRLLEELATSVDLVEGSVRIWDA
                     SSGEELQVYTSPRGPLYDVAFTPDGKKVIASGMGFVCGYIFDTQELIRLANSRLTRWF
                     TLDECRQYLHQEKCPPR"
BASE COUNT          542 a          703 c          647 g          522 t
ORIGIN      
        1 gcgattgtct ctcaccccaa gccgcaagcc tcagtttctc cccatcattg gatagctcaa
       61 tccacccatt gcacgcggtg cgcagcaggg tataggcggc gatgccgagg ccggctgcga
      121 taacgccgcg ccagcggctc agcgtggagg gcatttttcc tggtgaaggt actggcggca
      181 ctcatctaac gtgaaccagc gcgtgagacg agaattggcc aggcgaatca attcctgcgt
      241 gtcgaaaata tagccacaga caaaccccat gccggaagcg attaccttct tcccgtctgg
      301 ggtgaaggcc acgtcgtaga ggggtcctct cgggctggta tacacctgta gctcctcccc
      361 cgaagatgca tcccatattc tgacactgcc ctccaccagg tcgacagaag ttgcgagttc
      421 ctccaataac ctcggaggct tttcacacac atcttcggta caatcatggc cggctgaggc
      481 caggtatttc ccgtccgggc tgaaatccac gccttcaacg atgccggtat ggccaacgag
      541 atttagcaac tcttcgccgg ttttcgcatc ccaaatcttt aggctgccat tggctgaact
      601 cgttgctgcg cgctccccat ccgggctaat ggctatgccc gaaatgaatc tgatatatct
      661 ctgacctgaa aaagcggata tctccttgcc gctcgccgca tcccagattt tcgccaattc
      721 ataaggcgca tcattggttg ttgctagaag gaggccatcc ggactgaaag caagccggac
      781 aatgcccaaa cgatctggat ttgtctggat ggaaagcagc tcctggcctg tgctcacttc
      841 ccagatcttc gccactccat ccacgccgcc cgtggcaagt ttcgcgccat ccgggctgaa
      901 ggcgaccgaa gtgagtccgg ggaatttatg gttgactagc cagcttttga tgtgcccgct
      961 caaagtgtgc agcagctcca ccgtggccgc ccccggagag aggcgaagtt tccagatttt
     1021 ggcgatatta tcatctcctg cggtcgccag cagggtgcca tcggggctga tggccacgcc
     1081 gtacagcgga ccgccatggt tggtgatggc gagcagctcc cttcctgaag ccaggtccca
     1141 cactttggca accccatctg agctggctgt ggccaggtac ttcccatcag ccgtgaggtc
     1201 aaaaagatag ataggaccct gatgagcggc taatgtgaac caatccgagg cgcctgcggg
     1261 agtgatatcc cagatgcgca cgctgccatc tgcgctgctg gtagccaatc gtgtgccatc
     1321 gggactaaag gcaacgtcag ttagctgatc agtatgtccg gataaaacca tgagcagttc
     1381 cagaccttca gaagagggac gccacacctt agccagaccg tcttgaccga ccgaagccag
     1441 tacggatccg tccgggctaa aagcaaaatt gttaatcatg gacgtgtggg ctgcagatac
     1501 tacgagtggc ttcaatgacg ataaatccag agactcgagg tttgcattgg agatatccca
     1561 caagacaatc gcgttggtat gaattcccgc tcccgtcgaa accagccact tgccatccgg
     1621 gctgaagatc attttatcgt tactcggcag ttggtttagc agggcccaat cacggtctac
     1681 tccccaaaaa ctgatcttat ttccagattc cgtaatgtcc acggagatca gcagcctgcc
     1741 atccgggcta aaagccacat tattcagagc atcgaatcct tcgacctttg cggtcgagga
     1801 gaagatctct tgaccggtat ctgtatccca gacaataaag gtgttatcca cgctggagga
     1861 aactaaaagc tcgccatctg ggctgaaatg cacatcgaaa atcctccagg tatgacctct
     1921 ttttgtccac agcctgttcc gggtggcaag attccaaatc gtgagcgtgc catccaggct
     1981 gccggtagcc aagcgtactc cgtctgggct atagcgcgca acagttccgg gcaggctgaa
     2041 aagcacctgc ccgctacccg tatcccagat ggtcgcttcc catgcatagc tggttgtggc
     2101 gatggtcttc acatccgggc tgaactcgac tgagcgcacc catccggtat gctcgatcaa
     2161 tgccattctc actcgcgatg tttggaggga gcgatgcatg gcgtctttcg cttcgcgggt
     2221 atcgaccgta cgcagggctt gcagggacaa taagatgctg cgctctggat cgacctccag
     2281 gttgttgatc gccgcaacag ccagctcgcg ggaggtcgac agatcagcct gctttcgagc
     2341 gttccaggcc aacgccaggg aacccagggt agccaggagc aagacggcca ccagggctag
     2401 tagaaagcgg cgcg
//
LOCUS       DBNHOH010000146         2363 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1610776, whole genome shotgun sequence.
ACCESSION   DBNHOH010000146 DBNHOH010000000
VERSION     DBNHOH010000146.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1610776"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="AC16V7_02655"
     CDS             <1..558
                     /locus_tag="AC16V7_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HIR6256048.1"
                     /translation="RQYVSQRSNRPGEYWQSDPDAEYEAVYEFDVSQFEPYVAVPFLP
                     ENVKPVSELSEIKIDQVAIGMCTNGNLEDLRAAAEVLRGRQIHPNVRAVIIPGSQKVY
                     LDALKEGLIEIFLEANCSISTPTCGPCLGGHMGVLAAGERCVSTSNRNFRGRMGHPES
                     EVYLANPYVAAASAITGHITSPSAL"
     gene            571..1020
                     /locus_tag="AC16V7_02660"
     CDS             571..1020
                     /locus_tag="AC16V7_02660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HIR6256049.1"
                     /translation="MSSKTQGAEIDRTAAVAKFMEERDLFARHLGIQLLDLKPGYSRV
                     SMDLQPHMLNGLGMPHGAVIFALADFAFATACNSYGQAAVALSMDIHFISSPHPESSL
                     VGEAVEVQRSKRTGLYRLTVCDQEGKLVAELHGMAYRKDIDFLEGKG"
     gene            1017..>2363
                     /locus_tag="AC16V7_02665"
     CDS             1017..>2363
                     /locus_tag="AC16V7_02665"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR6256050.1"
                     /translation="MNFETLHQATLGELLSMQSIRYSNQPFLLMDDQQLTYAEVELQA
                     SALSAGLRELGLQPGERLAVILPNIPAYVITFFAAAKAGLIVIPINVRRHETELLERL
                     TSTKPAALITSSDTGQHSDIDHLAVALDIRHQLPSLKHVIAYGGGEGDVIAWESLLAY
                     TAPLPAPTIGLNSPAAIMYTLGSSGKPHGAILTHGGLVRNAASIAQTMECTPEDIFLG
                     ALPFSNTFGLTATILACATVNAQLVCLPRYAPEAALRLIDQKKISVHSGVPTMFALEL
                     NHASFKTSMCASLRTGIMSGAACPPELVKRVRAEMGLNLILAYGLTEASPSVAMTRLD
                     DGPVTTTETVGRPMEGVEIKVIDPDGNTLPPGETGELCVRGYNIMLGYWDEPEETSRV
                     LDKDGWLHTGDQATIDPNGPVRILGRMDEVINRGGFKIYPGSVEMVLRAYPGVKEAT"
BASE COUNT          527 a          658 c          655 g          523 t
ORIGIN      
        1 cgccagtatg tcagccagcg cagcaatcgc ccgggggaat actggcaaag cgaccctgat
       61 gctgaatatg aggcagtcta tgaattcgac gtatcgcaat ttgagccata tgtagctgtc
      121 cccttcctgc ccgaaaatgt caagcccgtc tcggagctct ccgagatcaa aattgaccag
      181 gttgccatcg gtatgtgcac caatggaaac ctggaagatc tacgcgctgc cgctgaggtg
      241 ctgcgcggcc ggcagatcca ccccaatgtg cgcgccgtaa ttattcccgg gagtcaaaaa
      301 gtgtacctgg atgcgctcaa agaggggctg attgagattt tcctggaagc caactgttcc
      361 atcagcaccc caacctgcgg gccgtgtctg ggcgggcaca tgggcgtgct ggcagccggc
      421 gagcgctgcg tctcgacaag caaccgcaac ttccgcggga gaatgggaca cccggaatct
      481 gaggtttacc tggcgaatcc ctacgtcgcc gcggcatctg ccattactgg tcatattacc
      541 tcaccatcgg ctctgtaaac acggagaagc atgagctcaa aaactcaagg cgccgagata
      601 gataggacag cggctgtggc gaagttcatg gaagagcgtg atctcttcgc ccgccacttg
      661 ggcatccagc tactggattt gaagcctgga tatagccgcg tgagcatgga tttacaacct
      721 cacatgctca atggattggg catgccccac ggtgctgtga tttttgccct ggccgatttc
      781 gcctttgcca ccgcatgtaa ttcgtatgga caggctgcag tagcgctaag tatggacatt
      841 cacttcatct cctctcccca tcctgaatcc agcctggtgg gtgaggcggt tgaagtgcag
      901 cgcagcaagc gaacaggtct ctaccgatta acggtttgcg atcaggaggg caaactggtc
      961 gcagaattac acggcatggc atatcgcaaa gatattgact ttctggaggg aaaaggatga
     1021 atttcgagac tcttcaccaa gcgacattgg gggaacttct gagtatgcag tctattcgct
     1081 actcgaacca gccctttttg ttgatggatg atcaacagtt gacttatgcc gaggttgaac
     1141 tacaggcttc agccctttct gctgggctga gagaattggg cttgcagcct ggcgaacggc
     1201 tggcagtgat cctgccaaac ataccggctt acgtgatcac tttcttcgca gctgccaaag
     1261 ctggtctgat tgtgatccct atcaatgtgc gccgccatga gacggaactt ctagagcggc
     1321 ttaccagtac caaacctgca gctctgatca cttcctccga tacgggacag cactcagaca
     1381 tcgatcatct ggctgtggca cttgacatcc gccaccaact tcccagcctc aagcatgtga
     1441 tcgcctatgg gggtggggaa ggcgatgtca tcgcctggga gagcctgctg gcgtacaccg
     1501 ctccactccc tgcgcccacc atcggtctca attcgcctgc ggctattatg tacaccctgg
     1561 gaagttcagg caaaccacac ggtgcaatcc tcactcacgg tgggctggtg cgcaacgcgg
     1621 ctagtatcgc acaaaccatg gaatgtaccc cggaagacat cttccttggg gcattgcctt
     1681 tttcaaacac ttttggactg accgccacca tcctggcttg tgctactgtc aatgcccagt
     1741 tggtatgcct gccgcgctac gctccagagg ctgccctgcg cttgatcgat caaaagaaga
     1801 tctcggtcca cagcggcgtg cccaccatgt ttgccttaga gctgaaccat gcaagcttta
     1861 agaccagtat gtgcgccagc ctgcgcactg gcatcatgtc tggtgcggct tgtccacccg
     1921 aattggtcaa gcgggtgcgc gccgagatgg gtttgaattt gatcctggcg tatgggttaa
     1981 ccgaggcttc tccatcagtt gccatgacac gccttgatga tggcccggtg accaccaccg
     2041 agactgtggg tcgccctatg gaaggcgtgg agatcaaagt gatcgatccg gatggcaaca
     2101 cgctgcctcc aggcgagaca ggcgagctgt gcgtgcgcgg ctacaatatt atgctgggat
     2161 attgggacga accagaagaa acttcccgcg tgctcgataa agatggctgg ttacacaccg
     2221 gtgaccaggc aaccatcgat ccaaacggcc cggttcgcat cttgggacgc atggatgaag
     2281 taatcaatcg aggcgggttc aaaatctatc cggggtcggt cgagatggtg ctgcgggcct
     2341 atcctggggt caaggaagcc acc
//
LOCUS       DBNHOH010000147         1776 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_842106, whole genome shotgun sequence.
ACCESSION   DBNHOH010000147 DBNHOH010000000
VERSION     DBNHOH010000147.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_842106"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="AC16V7_02670"
     CDS             <1..943
                     /locus_tag="AC16V7_02670"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6256051.1"
                     /translation="VMQKGVSNMHMASSMVWDWKADKVAKVPGVLEVSAILYLNGPVK
                     IGGKDWFSYIIGITPEYRRAGPWAMAQGKSLPDPGEAVIPEVISQLTGVQVGDEITMI
                     DRPLRVVGLSRGTFSMASSVVFVSHQDLGDLLEGRDQFSYILVYAEPGVSQQTLVESI
                     KDEVDKVNALGSEEFIESDRQLAVQMGAEIIHMMMILGTLLATLIVALTSYSLIARKK
                     PELAIAKALGFRNKQIYIAALSQSLLITGLGLLFTVIISFTLLTWLPAIAPQLNLAVR
                     LHQFAPLAVVALPVAALASLSAARTAVNVDPMIVFNS"
     gene            952..>1776
                     /locus_tag="AC16V7_02675"
     CDS             952..>1776
                     /locus_tag="AC16V7_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6256052.1"
                     /translation="MNSENILQAKHLTKIFGSGASEVRAVDDVSLNIKRNEMVLIMGP
                     SGSGKTTLLSMLGALLSPTSGEIIIDGIDVSAMNDTQVCALRAQKIGFIFQAFNLMEA
                     LSVEENILFPAQLTDKGIRGAKGHLDELLERLGLTARRSAMPNTLSGGEKQRVAIARA
                     LINQPPILLADEPTGNLDSHKGQEVMMILHDIARDDSCAVLMVTHDPRVEEIADRVLW
                     LEDGALSDRKSENHSWVQDPVCGMRVDEWTATLVGEYQNQRYVFCSTRCQERFENDP"
BASE COUNT          440 a          398 c          460 g          478 t
ORIGIN      
        1 ggtgatgcaa aaaggggttt ccaatatgca catggccagc tccatggtat gggactggaa
       61 ggcggacaag gttgccaagg tgccaggagt gttggaagtt tcggctattc tttatcttaa
      121 cgggccggta aaaattggtg gtaaggattg gttctcctat atcatcggaa ttaccccgga
      181 gtatcgtcgc gctggtccgt gggcaatggc acaaggcaaa tcgctacctg atcctggcga
      241 agctgtcatc cccgaagtaa tttcacaact caccggtgtg caggttggtg atgagatcac
      301 catgatcgat cggcctttgc gtgtggtggg gttgtcacgt ggcacctttt ccatggccag
      361 ctctgttgtt tttgtctctc atcaagacct gggagatttg cttgaaggta gagatcaatt
      421 tagttatatc ttggtttatg ctgagcctgg cgtctcccaa cagacgttgg ttgagagcat
      481 taaggatgag gtggataagg tcaatgcctt gggcagcgag gagtttatcg aaagcgacag
      541 gcaacttgcc gttcaaatgg gtgcggaaat aatacatatg atgatgatcc tcgggactct
      601 gctggcaacc ttgatagtcg cgcttacctc ctattcactc attgcccgca aaaagccgga
      661 gctggctatc gcaaaggcac ttgggtttcg caataaacaa atctacatcg ccgccttatc
      721 ccaaagttta ttaattacgg ggcttggatt gctctttact gtaatcattt cctttacact
      781 tttgacgtgg ttgccagcaa ttgcgccaca acttaatttg gctgttcgct tacaccagtt
      841 tgctccccta gctgtcgttg ctctgcccgt agccgcattg gcatcactta gtgccgcacg
      901 gaccgcagtc aatgtggacc caatgattgt ctttaattcc tgaagtctat tatgaattca
      961 gagaacattt tgcaagcaaa acatctaaca aaaatctttg gcagcggtgc ctctgaagtg
     1021 cgagcggtgg acgatgtaag cctgaatata aagcggaacg agatggtgct catcatgggg
     1081 ccgtcgggtt cggggaaaac aaccttatta tcgatgcttg gcgctttgtt aagccccacc
     1141 tcgggggaaa ttattattga tgggatcgat gttagcgcga tgaatgatac tcaagtatgc
     1201 gcactcaggg cgcagaagat tggttttatt tttcaggctt ttaatttaat ggaggcttta
     1261 agcgttgagg agaacattct cttccccgcc cagctcacag ataagggtat tcgaggggcc
     1321 aaaggtcatt tagacgaatt attggaaaga ctcggattaa ctgcgcgtcg cagcgccatg
     1381 ccaaacactt tatccggagg cgaaaaacag agggttgcca tagcccgtgc tttaatcaat
     1441 cagcccccta tcctcctcgc cgatgaacca actggcaatc tcgactccca caaagggcaa
     1501 gaggtgatga tgattctgca cgatatagca cgtgacgata gttgtgctgt actgatggtt
     1561 acccatgacc ccagagtcga agagattgct gatcgtgttc tttggctgga agatggcgca
     1621 ttgagcgacc gaaaatctga aaatcactca tgggttcaag acccagtttg tggcatgcgt
     1681 gtggatgaat ggactgctac tctcgtcggt gaatatcaaa atcaacgtta cgttttctgc
     1741 tcaacacgat gtcaggaacg atttgaaaat gaccct
//
LOCUS       DBNHOH010000148         5375 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_200818, whole genome shotgun sequence.
ACCESSION   DBNHOH010000148 DBNHOH010000000
VERSION     DBNHOH010000148.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5375)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_200818"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2249
                     /locus_tag="AC16V7_02680"
     CDS             <1..2249
                     /locus_tag="AC16V7_02680"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR6256053.1"
                     /translation="LLKLLPLPFVLVVVMGYPALGLGALVFLGTIPVVMEVLFYGTYS
                     IRRDLDRRSQELHTLNHISRTLRTAVDLNQLLEEVLNQVSQLFAVENFYIALYNPEEE
                     QLWYPIAVKHFQRVNWPPRALEDRLTDRVIRERSAILLPHNARDALASVGLPAAEDSP
                     TAWMGVPLISSERVIGCLGVFSFSPEVVFSKADVRWLTTLSGQVSVAIENMLRYQTAQ
                     RRADLALNQQVHQLTILEAVGRELAAAIRSDHLMDVILGYALEVTHSKWGSISLYEPI
                     ENLMVIKALRGYQKAIVSLSAEHGLCGRAVTTGQKIYAADVLQEPDYVDLTAGEARSQ
                     LCVPLLHESRVLGVLTLESEQVDGFSESDQAFINQLATQATVALVNAELYAQAQERIS
                     EFSAVFNSVGEGLLVLDSSGQITLANEAIRILTGLSPTEFSGQRLVDLPETILAYLGY
                     NSEQAEALVGAMGQGQVLASPRATIRISELNSERILERTSFPVWDRRGRVTGWMIVLR
                     NMTEEIQITQAKDLITETLVHDLRSPASAVLGALDVLKDSLNHDQTENEIVVQAIQVA
                     RRGAQRMLGMTESLLEISRLQSGKIETNLLNFSLRPMATSVLNDFKTEALEYGIILHN
                     DIPDDLSAVRADLNKTTRVLTNLVDNALKFTPAGGQISLSAAHLTDGFITVKVSDTGP
                     GVPEGYREKIFERFSQVPGLVGRRRGSGLGLTFCRLAIEAQGGKIWMEPRPGGGSIFM
                     YTLPVSRA"
     gene            2422..2721
                     /locus_tag="AC16V7_02685"
     CDS             2422..2721
                     /locus_tag="AC16V7_02685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR6256054.1"
                     /translation="MPVYTYRCENCGVQFDRHQSFSDLPLTRCPECSKKTLRKVYTPV
                     GIVFKGSGFYATDNRSPSGSSRSSTGSEKMSEGKKDKKESESSSSTNSDGKEKSA"
     gene            2814..4151
                     /locus_tag="AC16V7_02690"
     CDS             2814..4151
                     /locus_tag="AC16V7_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HIR6256055.1"
                     /translation="MKNEEMLARIREDQVKFISLQFTDVTGTVKSVDIPSGRLESALE
                     DGVWFDGSSVEGFARIQESDMRLVIDPSTYAVLPWTPQELRRARVFCDIYLPNGNPFP
                     GDPRGTLKRILAEIESRGWLFNVGPEPEFFLFRRNGEKPIHPVPHDVGGYFDFSANDD
                     AVRVRTELMNALSGMGLDVEMGHHEVALGQHEIDFRYADALRAADNILTLKYTVKAIA
                     AQHGLVASFMPKPIFGINGSGMHCHQSLFNKEGENLFFDANDEYNLTKLAYGFIAGQL
                     GHARALAAVVSPTINSYKRLVPGYEAPVYVGWAQINRSALIRIPRYTPGREKSSRAEL
                     RFPDPSANPYLALTVMLAAALDGIDNNLPCPPPLNNVNVYHLTPEERVAMNIPELPGS
                     LAEALRELENDKVLQAALGAEAYEAFYRAKWAEVEEYRMRVTDWEVERYLETA"
     gene            complement(4233..5192)
                     /locus_tag="AC16V7_02695"
     CDS             complement(4233..5192)
                     /locus_tag="AC16V7_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP-loop family protein"
                     /protein_id="HIR6256056.1"
                     /translation="MKCRKCEAKAIINMRQHKLALCKQHFPIWIAEQTQRFIEKYHMF
                     SQEDRLLVAVSGGKDSLSLWDILHQLGYKADGLYIGLGIDGGLHYSDESHRLANKFAI
                     ERNLHLRVVDVHETYGETIPEIAERTTRGKGRPCSVCGLTKRHVMNRIARDEGYDVLV
                     TGHNLDDEAAVLFANTLNWSPGYLMRQSPVLQARQGMVRKAKPLCRFYEREMAAYALV
                     RGIDYIYDECPYVEGSKAIYYKEILNKLEADRPGAKLSFYLSFLQAKESGLFTSPPET
                     LAEIGASGEILNSCPSCGQPTTAPGECAFCRMVIQVSAVNPTG"
BASE COUNT         1280 a         1289 c         1409 g         1397 t
ORIGIN      
        1 ccttgttgaa gttgctgcct ttgccttttg tgttggttgt ggtaatgggt tatccagcac
       61 ttggattagg tgcgctggtg tttttaggga ctattcctgt agtaatggaa gtgcttttct
      121 acggaaccta cagcattcgc agggatcttg atcggcgttc tcaagagttg cataccctaa
      181 atcatattag ccggaccctg cgaaccgccg tcgacttgaa ccaattatta gaagaggtcc
      241 taaatcaggt cagccagcta tttgcggtag aaaattttta tatcgcatta tataaccccg
      301 aggaagagca attgtggtat cccatagccg ttaaacattt tcagcgagta aactggccac
      361 cgcgtgcgtt agaagatcgt cttacagatc gggttattcg cgagcggagc gctatcttat
      421 tgcctcacaa tgcgcgtgac gctctagcgt ctgtaggtct gccggctgct gaagattctc
      481 cgactgcctg gatgggagtg ccattgattt cgtctgagcg tgttattgga tgcctgggcg
      541 tattctcttt ctctcctgag gtggttttta gcaaggcgga tgtgcgttgg ttgacgactt
      601 tatcgggaca ggtaagtgtc gccattgaaa atatgcttcg ttaccagact gctcagcgac
      661 gtgcagattt ggctttgaat cagcaggtac accaacttac tatccttgag gccgtaggcc
      721 gagagctggc ggcagccatc cggtcggacc acttgatgga tgtcattctt ggctatgcct
      781 tggaggtaac ccactcgaaa tgggggagca tctctctcta tgagcccatc gaaaacctga
      841 tggtgattaa ggctctgcgt ggatatcaga aagcaatcgt ttcattgtcc gcagagcatg
      901 gtctctgcgg tcgggctgta actaccggac aaaagatcta cgcagcggat gtcttgcagg
      961 aaccagacta tgtcgacttg acggcagggg aagccagatc acaactttgt gtgccattgc
     1021 tgcatgaaag ccgcgttctg ggggtgttga ctctagagag tgagcaagtc gatggctttt
     1081 cggaaagcga ccaggctttc atcaaccagt tagctaccca ggcaacggta gcgttagtta
     1141 acgctgaact ttacgcccaa gcgcaggagc gcatcagtga gttttcggct gtcttcaatt
     1201 cggtgggaga gggattgtta gtcctcgact cttcgggtca aataaccctg gctaatgagg
     1261 caatccgcat ccttactggt ctctcgccga cggaattttc tggtcagcgg ttggttgact
     1321 tgcctgagac gatattggct tacctggggt acaattcgga gcaagcagaa gctctggtag
     1381 gtgctatggg gcaaggacag gtgctggctt ctcctcgagc caccatccgc attagtgaac
     1441 tgaactcgga aagaatcctg gagcgcacat cctttccagt ttgggaccgc cgaggccgcg
     1501 tgacgggttg gatgatagtg cttcgcaaca tgaccgagga gattcagatt actcaggcca
     1561 aggatctgat cacggaaacg cttgtacacg atctacgctc acccgccagc gctgttctgg
     1621 gagctcttga cgtactaaag gactcactca accatgatca aactgaaaat gagatcgtgg
     1681 ttcaagcgat tcaggtagct cggcgtgggg cgcaacgtat gttggggatg actgaatcgt
     1741 tgttggagat ttctcgtctc caatctggta agatcgaaac caatttgcta aatttctcgc
     1801 ttcgacccat ggctaccagc gtgctaaacg atttcaagac cgaagcgcta gaatacggca
     1861 tcattctgca caatgacatc cctgacgatt tgtcagccgt gcgcgccgat ctaaataaga
     1921 ccactcgtgt gctcaccaat ttggtggata acgcacttaa gttcacccct gcgggtgggc
     1981 agatcagctt gagcgccgcc catctcacgg atggatttat cacagtaaag gttagcgaca
     2041 cgggaccggg tgtgcccgag ggctaccgcg agaagatctt tgagcgcttc agccaagttc
     2101 cgggcttggt gggtaggcga cgaggttcgg gtttagggct taccttctgc cgcctggcaa
     2161 ttgaagcaca aggtggcaag atatggatgg agcctcgtcc tgggggcggt agcatcttta
     2221 tgtataccct tccagtctcc cgtgcgtaga gttaaatttc tgtagacccc caaacaactc
     2281 atttcactta atgaagtttc ccctgattgg gggattaaat catttcatat aaatttctga
     2341 tgcaccttcc ccttgtcaac ctatttcctg cgcgctataa tcaagttttg tgatcggtcg
     2401 caatagataa ggtaagaatt aatgcctgta tatacctatc ggtgtgaaaa ttgtggtgtg
     2461 cagtttgatc gacatcagtc atttagcgat ctgccgctta cccgctgtcc ggagtgtagt
     2521 aaaaagacac tgagaaaagt ctacacgcct gtaggaattg tatttaaagg gtctggcttc
     2581 tacgccactg acaaccgctc tccatctggg tcgagccgct cttctacggg aagtgagaaa
     2641 atgagtgagg gtaagaagga taagaaggag agcgaatcga gctcatcaac caattcggat
     2701 ggtaaggaga aatctgcata atggtagaag atagctttag gctatccatt taacaatcta
     2761 cgcttgagac gcctggatat tgaggcgtct cattttataa ataaggagac gctatgaaga
     2821 acgaagaaat gcttgctcgt atccgagagg atcaagttaa atttatatcg ttgcaattta
     2881 ctgatgttac tgggacggtc aaaagcgtgg atatccctag tgggcgtttg gaatccgcat
     2941 tagaggatgg tgtatggttt gatggctctt cggtggaggg ttttgcccgt atccaggaga
     3001 gcgacatgcg cttggtgatt gaccccagca cctatgctgt tctgccgtgg acaccccaag
     3061 aactgcggcg ggcgcgtgta ttttgtgata tctacttgcc caatgggaat ccattcccag
     3121 gcgatccacg cggcacgctt aagcgtatac tggcggagat tgagtctcgc ggctggttat
     3181 ttaatgtagg acccgaaccc gagttcttcc tcttccgaag gaacggagag aaacccatcc
     3241 accctgtgcc acatgatgtg ggtggttatt tcgatttctc tgccaacgat gatgcagtgc
     3301 gcgtacgcac agaacttatg aatgccttat cgggaatggg tctcgatgtg gagatgggac
     3361 accacgaagt ggctctaggg cagcatgaga tcgattttcg ctatgcagat gcattacggg
     3421 ctgcagataa tattctgacc ctcaagtaca ctgttaaagc tattgccgct caacatggat
     3481 tagtggcttc attcatgccc aaaccgatat ttgggataaa cggatcgggt atgcactgcc
     3541 accaatcgct ttttaataag gaaggtgaga atttgttctt cgatgcaaac gatgaatata
     3601 atctcacaaa gctagcctat ggtttcatcg ctggtcaact ggggcatgca cgcgcgttag
     3661 cggcggttgt ctctccaacg atcaactctt acaagcgact tgtaccgggt tacgaagccc
     3721 cggtgtatgt tggttgggcg cagatcaatc gctcagcgct catccggatc ccccgctata
     3781 cgcctggtag ggagaaatct tcgcgagctg aactgcgctt tccggatcca tctgcaaatc
     3841 catatctggc tctcaccgtt atgctggcag ccgcattaga tggcatcgat aacaatttgc
     3901 cctgtccgcc tccgctcaat aatgtaaatg tttatcattt gacgcctgag gagcgcgtgg
     3961 cgatgaatat tcccgagttg ccgggctctt tggccgaagc cttacgagaa ctcgagaacg
     4021 acaaagttct ccaggctgca ctcggagcag aggcctatga agccttttac cgtgccaaat
     4081 gggcagaggt agaagaatac cgcatgcgcg tgacagattg ggaagtcgag cgctatctcg
     4141 agacagcata ataacgaaaa aaacggatgg cgaaaggatt cttgctttcg ccatccgttt
     4201 ttaattcctg cttcactcaa ttgttaataa ggttacccag tgggattcac cgcggatacc
     4261 tggattacca tgcggcaaaa tgcgcactct cccggggcag ttgtcggttg accacaactg
     4321 ggacaggagt ttaatatttc acctgaagcg ccgatttctg ccaaagtctc gggtggggag
     4381 gtgaacaagc cgctttcttt ggcctgcaag aaggacaggt aaaaactcag cttcgcacct
     4441 ggacggtcgg cttctagctt attgagaatt tctttgtagt agatggcctt tgatccctcg
     4501 acgtaagggc actcatcgta gatgtagtcg ataccccgca cgagggcata agcagccatc
     4561 tcgcgctcgt agaagcggca aagtggctta gctttccgca ccatcccttg gcgcgcttgc
     4621 agcactgggc tctgacgcat gagatagccc ggagaccagt tcaatgtatt ggcaaataat
     4681 acggcggctt catcatccag gttatgtcca gtgaccagta catcgtagcc ttcgtcacgt
     4741 gcgatgcggt tcatcacatg gcgtttggtc aggccgcata ccgaacaagg gcgacccttg
     4801 ccgcgtgtgg tgcgctcagc tatctcaggg atggtctctc cataagtctc atgcacatct
     4861 actacccgaa ggtgaaggtt gcgctcaata gcaaatttgt tcgccaggcg gtgcgactcg
     4921 tcggagtagt ggagtcctcc gtcaataccc aggccaatgt acaagccatc tgccttgtaa
     4981 cctaattggt gcaagatatc ccacaaggag agagaatcct tgccacccga aaccgcaact
     5041 aacaagcgat cttcttggga gaacatatga tacttttcga tgaagcgctg ggtttgctct
     5101 gcgatccaga tgggaaaatg ttgcttgcat aaggccagtt tatgctggcg catattgatg
     5161 atggctttgg cttcgcattt acgacatttc attttctatc ctaaccaata attttttccc
     5221 ttagcgcgat gaacaacaac tcatccgtcc aattattccg tactctgtcc tccagaaatt
     5281 acggcgacca actttatgac atcatcttcg agcacgattt catcatcggt gatgagctca
     5341 cctgcccggg tcgcaagcac cgtttcgctg ttgac
//
LOCUS       DBNHOH010000149         3560 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_8825, whole genome shotgun sequence.
ACCESSION   DBNHOH010000149 DBNHOH010000000
VERSION     DBNHOH010000149.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_8825"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..119
                     /locus_tag="AC16V7_02700"
     CDS             <1..119
                     /locus_tag="AC16V7_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6256057.1"
                     /translation="WHIVVYPGMAIVLFVLAWNLVGDALRDIMDPRLRGRGA"
     gene            137..901
                     /locus_tag="AC16V7_02705"
     CDS             137..901
                     /locus_tag="AC16V7_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6256058.1"
                     /translation="MAHSCAPSAFKQDVQDRQDRRKILLILYILLITLACTLNPARAV
                     GAQGTTQPPPTPMPMMPPMPDGLEPPPTVYPPTQASQGAQVYYLVCMACHGDKGQGLT
                     QEWLNELNPFDRNCWQSKCHASNHPPEGFVLPKFVPAIIGPGEMDGYPTALGLHDYIQ
                     ARMPWQAPGSLSEDEYWQLTAFLVQANGFELGAEPLDRERAAKVFLGLKPTPTIAPAL
                     FLESSGVGFWVVSGGVLALIALLVILKLRAGLGRNN"
     gene            1009..1590
                     /locus_tag="AC16V7_02710"
     CDS             1009..1590
                     /locus_tag="AC16V7_02710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6256059.1"
                     /translation="MLEVTDKALVIQLQEGNLEALGALYDRHRRLVYRTALAITGDAD
                     SAADLLQDVFLRLNRFSRNIDPLRPLEPWLYRMTTNLSYDWVKRRKRWPRPLEDLADW
                     LIGSVKNSPYEVVEKNDEWSRVQRAVAALPLPQRVVVVLYYLNDLALQEIAEILDVPI
                     GTVKSRLYYGRLGLKRSLGLEQDLVPDLQFEQT"
     gene            1601..1849
                     /locus_tag="AC16V7_02715"
     CDS             1601..1849
                     /locus_tag="AC16V7_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256060.1"
                     /translation="MKNLFDATIKKAMKEWADQHEPPKQGKARLLMLAATSASKHIHT
                     PTFRKRDFYSLSQTSSNLASSMVAEKFHSPWLWMFNVA"
     gene            1946..>3560
                     /locus_tag="AC16V7_02720"
     CDS             1946..>3560
                     /locus_tag="AC16V7_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6256061.1"
                     /translation="MIKPSQLFSPFPWIVLVFLLSSCTLPTSTPIPETTPLDPTAQSP
                     TLQALIPPTTPPPRALTVCLGQEPTSLFIYADGSSAARSVRQAIFDGPAEIVNYELQP
                     VILERLPNLANADVLLEPTQVVSGTLIVASDGSLANLEEGVVFLPAGCNRAECAQSYA
                     GEAAVSMEQLVVRFRLRPGLLWADGVALTAADSVFSYEVARALYPRARPNLIDFTHSY
                     LALDELTVEWRGLPGYKDPLYQTNFFVPLPQHLWGSIPPGELLSAEISSRMPLGWGPY
                     MIEEWTSGDHITLIKNPNYYRASEGLPYFERLVYRFIGQGETALSALLAGECDFVDES
                     GLDGIPHQRLFQLQDEGRIKVIAEPGTAWEHADFGLASLDAEKLSLFQSREVRQAIAQ
                     CIDRQALAESLFYGKSVSLNTFVPLTHPLYNNEAREYAYDPQAAASLLEAAGWVDADG
                     NTGTPRIAQGVPGVPDGTPLVFAYQTLGGGERQGAAELIRDWLAQCGIAVNLQYVERE
                     TLFAPGPDGPLFGRRFDMAQFAWPASLQPA"
BASE COUNT          748 a          967 c          995 g          850 t
ORIGIN      
        1 actggcatat cgtcgtctac ccaggaatgg cgattgtctt attcgtgctg gcctggaacc
       61 tggtgggcga tgcattgaga gacatcatgg atccacgcct gcgcggccgg ggggcgtaat
      121 aaaaaaacgg tttatcatgg cgcactcgtg tgcgccatct gcttttaaac aggatgtaca
      181 ggatcgacag gatagaagaa aaatcctgct tatcctgtac atcttgttga ttaccctggc
      241 atgtaccctc aaccctgcaa gggcggtggg tgctcaaggc acaacccagc ctccacccac
      301 cccgatgccg atgatgcccc ccatgcctga tgggctagag ccgccgccca cggtataccc
      361 accaacccag gccagccagg gcgcccaggt gtattacctg gtatgtatgg cctgccacgg
      421 cgataagggg caaggtctca cccaggagtg gctgaatgag ctaaaccctt tcgaccgcaa
      481 ctgctggcaa tccaaatgcc acgcctccaa tcacccgccg gaaggctttg tgctacccaa
      541 atttgtgcct gctatcatcg gtccaggcga gatggatggc tatcccactg cccttggctt
      601 gcacgattac atccaggcgc gcatgccctg gcaggcgccg ggtagcttga gtgaagatga
      661 atattggcaa ctcaccgctt tcttagtgca ggcgaacgga tttgagcttg gggcggagcc
      721 cctggatagg gaacgagcag ccaaggtgtt cctgggcctc aaacctaccc cgaccatcgc
      781 gccagccctg tttctcgaaa gctccggggt tggcttttgg gttgtctctg gtggggtgct
      841 ggctctcatc gctttgctgg tgattctgaa gctgcgcgct ggtttgggta gaaataattg
      901 aagttttgct gctgcttttt ggtataataa tcaaaggttt gggtttcttt tattggtttc
      961 gggtggtagc cgtctaaaag tgattgtata agcatgggtg aggtgataat gctagaagtc
     1021 acagataagg ctcttgtcat tcagctacaa gaaggtaatc ttgaagcact gggcgcgctc
     1081 tatgatcgcc atcgccggct ggtttatcgg actgcgctgg cgattacggg ggatgcggat
     1141 tcagccgctg atcttttgca agatgtattt ttgcgcttga atcgtttttc gcgtaacatc
     1201 gatccgctgc gcccgctgga accctggtta tatcgcatga cgactaatct gtcgtatgac
     1261 tgggtgaaac gccgcaaacg ttggccacgc cccttggaag atctagcaga ttggctaatt
     1321 ggctcagtga aaaactctcc ttacgaggtg gtagagaaga acgacgaatg gtcccgggtg
     1381 caacgggcag ttgccgcact gcccctgccc cagagggtag tagtggtttt atattacctc
     1441 aatgatcttg cccttcaaga gatcgctgag atcctggatg tgcccatcgg cacggtgaaa
     1501 tcccgcctgt attatggtcg ccttggattg aaaaggagcc tggggctgga acaggatctg
     1561 gtaccagact tacagtttga gcaaacatag gtgaagtgag atgaagaatt tatttgacgc
     1621 gacgattaag aaagcaatga aggagtgggc ggatcaacat gagcccccga aacaaggaaa
     1681 ggcgcgctta ttaatgcttg cggcgacaag cgcttcaaaa catatccata cccccacatt
     1741 tcgcaaacgc gatttttact cgctatcgca aacgagttcg aatctagctt ccagcatggt
     1801 cgctgaaaag tttcatagcc cctggctatg gatgttcaat gtggcctgat tttgcagcct
     1861 cttggctggg tcagcagttc aactgctgac ccagttgtgt gcaagccccc attttatcga
     1921 aactcattcc ctataaggtt gccctttgat taagccatcg cagctattct cgccattccc
     1981 ctggatagta ttggtatttc tcttaagttc ttgcacgctt cctacatcta cccctatccc
     2041 cgaaacaacc ccactagatc ctactgctca atcacctacc ctgcaggctc ttatcccacc
     2101 gacaactcca cccccgcgcg ccctgacggt ttgcctgggg caagagccca catcgttgtt
     2161 tatatatgca gatggctctt cagccgcccg tagcgtgcgc caggcaatct ttgacggacc
     2221 agcggagatc gttaattatg agctccagcc ggtgattttg gagcgcttac ccaacctggc
     2281 aaacgcagat gtccttcttg agccaaccca ggtggtcagc ggcacgctga tcgtcgccag
     2341 cgatggtagt ctggctaacc tggaggaggg cgtggtgttc ttgccggcgg gctgcaatcg
     2401 ggctgagtgc gcccaatcct atgccggaga ggctgccgtg agcatggaac aactggtggt
     2461 gcgcttccgg ctgcgacctg gtctgctgtg ggccgatggg gtcgctttaa ctgccgctga
     2521 ttcggtcttc tcgtatgaag tagcgcgtgc cctgtacccg cgcgcgcgcc ccaacctgat
     2581 cgactttacc cattcctatc tagcactgga cgagctgaca gtggaatggc gcgggcttcc
     2641 cggttacaaa gatcccctgt accagaccaa cttctttgtt ccgctaccac agcacctgtg
     2701 gggtagtatt ccccctggtg aattgttgag tgccgaaatc tccagccgta tgccacttgg
     2761 ctggggaccg tatatgatcg aggaatggac atctggcgat catatcacgc tgattaagaa
     2821 tcccaactac taccgcgcct ccgaggggct gccgtacttc gagcgcctgg tgtatcgatt
     2881 tattggtcaa ggagaaacag cactttcagc gctgctggca ggcgaatgcg attttgtgga
     2941 tgaaagcggg ctagacggca ttccgcacca gcgcctgttc cagctgcaag atgaagggcg
     3001 tatcaaagtg attgcagagc ctggcacagc ctgggaacac gctgattttg ggctggcatc
     3061 gctcgatgcg gagaaactta gcctgttcca gtccagggaa gtacgccagg caattgccca
     3121 gtgcatcgac cggcaggcgc tggccgagag tctgttttat ggcaagtctg tgtccctcaa
     3181 tacatttgtg ccccttaccc accctctcta taataatgag gcgcgtgagt atgcatacga
     3241 ccctcaggcg gcagcctcgc tgttggaagc cgccggctgg gtggatgctg atggaaacac
     3301 ggggacgccc cgcattgccc aaggcgtgcc gggtgtaccg gatggcactc ccctggtttt
     3361 tgcttaccag accctggggg gaggtgagcg gcagggagca gccgaattga tccgggattg
     3421 gctggcgcag tgcggcatcg cggtgaatct gcaatacgtt gagcgagaga ccttattcgc
     3481 gcccggcccg gatggccccc tgtttgggcg gcgcttcgat atggctcagt ttgcttggcc
     3541 cgcttccctg caaccggcct
//
LOCUS       DBNHOH010000150         3674 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_714073, whole genome shotgun sequence.
ACCESSION   DBNHOH010000150 DBNHOH010000000
VERSION     DBNHOH010000150.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_714073"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3674)
                     /locus_tag="AC16V7_02725"
     CDS             complement(<1..>3674)
                     /locus_tag="AC16V7_02725"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR6256062.1"
                     /translation="VRIQHLGLEHFAVAAGVRQTVLVPLTSSGHMLGYLQAANKRDGS
                     DFDQSDLRLLAIIAGQAAPIIENAGLVHESSRRAQRAEMLRRIANLTASSAKLDEILK
                     LSLQELAQLLGADMALMLLFDEGKGELRLHRPSLFGISPDVTLRLGRLPVDDPEFPNT
                     ATGSLKSLLVGDVMDEPDVISTYRNTAINLQGRSILVTPMVLRKRGIGELLLIGRDVA
                     SFNPAELQVVAAAADQLAGAIERADLYAQTDESLRRRVDQLTAVTRVSRELSSTLQLE
                     QLLQRVFEEVLRTTRADCGTIVLFALHEADFQNNVSSSSPRVLLRLGESKSGLHSLER
                     MVLKRGETLILNDFNPLRDVVDALEAGIELAQPPHEGIQSAMVIPIFYQDQIAGLIHL
                     HARAPFRFDESAREIGETLAIQASIAIGNAQRYQEQYHRSELLDRRMGTIAKLLETSQ
                     AWQLELPLEKSMEEIASAIQGATPFDVVVISIYNPDDQKLHRLANAGLSLTDMEELRA
                     HPQSWGKLQDVLKPEFQIGRSYFIPVEQMPVMPPDVHSLIIQPVEDAATDEADHPGRV
                     WHPEDALVVPLHSAQGEPLGLISVDLPRDHLRPDLPTIETLEIFASHARLVIESQREY
                     SRLQQQLDTIQHELQLSKEVAETAQANLPVLLQKDIDQSLALRRLGQHARRIRAGIDI
                     ADLANSQSDRSSVLRTLGNEFLTRLNLDVILVVEPGLGGPHLLEALGELPSEINLEAL
                     IGQRNPLYHCLRTQDQLLVDRLESKMKWADAPLLQALDVQSFICFPILSGDRLDAAVL
                     GVSRSPMDPFTEDDEQIFTLLSRQVGITIQNLQLLGETSQRLAEVNLLLDFNRRLGGL
                     NPTGIAHTLGQSILQLVPNSQAVLVALWDTQRRALVPQDAIGYADATRMMEIIYNPGE
                     ALPGQVFESGQAVILDEVDFAKHYSLTSENLLRFRNATGGKLPVSTIVAPILPAEGAQ
                     ALGIVVVDNFQMSAAFTPEILALVTSLAQQAALNLENTRLVQAAEERASQLQALTGVA
                     ATITSNLQPDNLIAMLLSLLGAILPYDTGTLWLKQDENEMIVRSAHGFSDNEQRIGLT
                     VALQDSILLSDMIASGQPVAVGDIRHDARFSSLIEPQYISWLGVPLITSGEVIGVIAL
                     EKSESNYYTQEHRQIATTFASQAAVALENARLYHESLNRTLELDQRSQRLEMLNRLSA
                     ALIATLEPEHILQVATQELG"
BASE COUNT          889 a         1011 c          950 g          824 t
ORIGIN      
        1 gccctaactc ttgggttgcc acctgcaaga tgtgctccgg ctccagggtt gcaatcaagg
       61 ctgcagacaa gcgattgagc atctccagtc gctgcgagcg ttgatccagc tccagggtac
      121 ggttgaggct ctcgtggtaa agacgggcat tttccagcgc tactgcagcc tggctggcaa
      181 atgtcgtcgc aatttggcgg tgctcttgag tgtaataatt ggactcggac ttctccaggg
      241 cgattacgcc aatcacttcg ccgctggtga tcaggggcac acccagccag gaaatatatt
      301 gcggttctat taaggaggaa aagcgcgcat catggcgaat gtcacccacc gccaccggct
      361 gcccgctggc gatcatatcg ctgagcaaaa tactatcctg taatgcaaca gtcaatccaa
      421 tacgctgctc attatctgag aaaccgtgcg cagagcgcac gatcatttcg ttttcatcct
      481 gcttaagcca tagggtgccc gtatcatatg gcaggattgc acccagcaag ctcaaaagca
      541 ttgcaatcag gttatcaggt tgcagattag atgtaattgt agcggctaca ccggtcaagg
      601 cctgcaattg gctggcgcgc tcttcagcag cctgcaccag gcgagtattc tctaaattca
      661 aagctgcctg ctgagctagc gaggttacca gcgccaatat ttcaggagta aaagcggctg
      721 acatctggaa attatccacc acaacgatgc ccaaagcctg cgccccttcg gcgggcagga
      781 taggcgcgac tatggtagat accggcaact tcccaccggt tgcattacga aagcgcagaa
      841 ggttttcgga cgtaaggctg taatgcttgg caaaatccac ctcatccaaa attacagctt
      901 gaccactctc aaaaacctga ccaggcaagg cttcgccagg gttgtatata atttccatca
      961 tccgcgtggc atctgcgtat ccaattgcat cttgagggac aagggcacgc cgctgagtat
     1021 cccacagtgc gaccagaaca gcctggctgt tgggtaccag ttgcaaaatg ctttgtccca
     1081 gggtgtgagc gatgccagtc ggattcaacc cccccaagcg gcgattaaag tccagcagca
     1141 ggttgacttc tgccaggcgc tggctggtct cacctaacag ttgtagattt tgtattgtta
     1201 tccctacctg gcgggagagc aaggtgaaga tctgttcatc gtcttcggta aatggatcca
     1261 ttggagagcg acttactccc aagacagccg cgtccagccg atcgccgctc aagattggga
     1321 agcaaataaa actctgcacg tcgagcgcct gtaataaggg tgcatctgcc catttcatct
     1381 tgctctccag gcgatcaacc agcaattgat cctgggtgcg caggcaatga tatagcgggt
     1441 tgcgctgccc gatcagtgct tccaaattga tctcgctggg caattcaccc aaagcttcca
     1501 gcaagtgtgg tccgcctaat cctggctcaa ctaccaagat aacatccaaa tttagacggg
     1561 tgaggaactc gttgcccaat gtacgaagca cgctcgagcg atcactttgg ctattagcca
     1621 ggtcagcaat atcgatgcca gcacggatgc gccgggcgtg ttggcctaat cggcgcagag
     1681 ccaaggattg gtcaatatcc ttttggagca aaacgggcag gttcgcttgt gcggtctccg
     1741 cgacctcttt agacagttga agctcatgct ggatggtatc cagttgctgc tggaggcggg
     1801 aatactcgcg ctggctttca ataaccaacc ttgcgtggct agcgaatatc tcaagcgtct
     1861 cgattgtcgg aagatcgggg cgcaaatgat cgcgaggtaa atctacgctg atcaatccca
     1921 aaggctcccc ttgagcagag tgcaaaggga ccactaatgc gtcttcagga tgccaaaccc
     1981 ggcccggatg gtcagcctca tctgtagctg catcttcaac aggttggata atcaacgagt
     2041 gcacatctgg tggcattacc ggcatctgct ccacgggaat gaaatacgag cgcccgattt
     2101 ggaattccgg cttcaaaaca tcctgtaatt taccccaaga ttgcggatga gcacggagct
     2161 cctccatatc cgtcaaagac aagcctgcgt tcgcaaggcg gtgcagtttt tgatcatccg
     2221 ggttgtagat gctgatcacc accacatcga acggagtcgc cccctggatt gcggaagcaa
     2281 tttcctccat ggatttttcc aggggcaatt ccaactgcca ggcttgcgaa gtttccagta
     2341 gtttcgcaat agtacccatg cggcggtcga gcagctcgct gcggtgatat tgttcctggt
     2401 agcgttgtgc gttgccaatc gctatggaag cctggatagc aagtgtttca ccaatctcgc
     2461 gcgccgactc atcaaaacga aatggcgctc gggcatgcaa atgaatcaac ccggcaattt
     2521 gatcttgata gaagataggg atgaccatcg ccgattggat cccctcgtgg ggaggctgag
     2581 ccagctcaat tccagcttct agcgcatcta ccacgtccct tagcggatta aagtcattta
     2641 aaatcagcgt ctcgcccctc tttagcacca tgcgttccag gctgtgcagc cctgacttcg
     2701 attcaccgag gcgtagcaat acgcgtggtg aagaagagga aacgttgttt tggaaatccg
     2761 cttcatgcaa cgcaaagagg actatcgtcc cacagtcagc acgtgtggtg cgcagcacct
     2821 cttcgaagac gcgctgcaac aactgctcca attgcaaggt gctgcttagt tcccgactga
     2881 cacgggtgac tgcggtcagt tgatccaccc ggcggcgcaa gctttcatca gtctgcgcat
     2941 acaagtcggc gcgctcaatc gctccagcca gctgatcggc agcagcagca accacctgca
     3001 attcggcagg attgaagcta gccacatccc gtccaatgag caataactcc ccaatacctc
     3061 gcttgcgcaa caccatgggg gttacaagta tcgagcgacc ctgaaggtta atggcggtgt
     3121 tgcgataggt cgatatcaca tctggctcat ccatcacatc ccccaccaac aaagacttta
     3181 aactacctgt ggcagtgttg ggaaattcag gatcatccac tggcaaacgc cctaatcgca
     3241 gagttacatc gggggagata ccaaatagcg aaggtcggtg aaggcgcagc tcacccttgc
     3301 cctcatcaaa aagaagcatg agcgccatat cagcccctaa caactgggcg agctcttgta
     3361 gagagagctt aaggatctca tccaattttg ccgaggaggc tgtcaaattg gcaatgcgcc
     3421 ttaacatctc ggcacgctga gcccggcgac tggattcatg caccagccca gcattctcta
     3481 taatcggcgc agcttgcccg gcgataatag ccagcagccg tagatcgctt tgatcgaagt
     3541 cactgccatc tcgcttgtta gctgcctgga ggtagccaag catgtgaccg ctgctggtga
     3601 gtggcaccag caccgtctgg cgaacacctg ctgctacggc aaagtgttca agccccaaat
     3661 gctggatgcg caca
//
LOCUS       DBNHOH010000151         2783 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1482524, whole genome shotgun sequence.
ACCESSION   DBNHOH010000151 DBNHOH010000000
VERSION     DBNHOH010000151.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1482524"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..2110
                     /locus_tag="AC16V7_02730"
     CDS             230..2110
                     /locus_tag="AC16V7_02730"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6256063.1"
                     /translation="MQQDYMLKEAIEAVREGYKVRARDLLTRLLRADQTNPVYWLWMS
                     SVVETSREQVYCLNNVLRLDPHNEAARRGLTLIGAMPADQDMNPTPLVRRQWHVAVQD
                     EPPQGIKALWANPVIRISFSGTLALVVIALIFVGIFGLGEKPIAVAARPTKTPGPPPT
                     FTSTPTYIGSTRTANKITPTPPVTGPLPLWMLLDATYTPTPVYVNTPHPISEDYRIGQ
                     RAFGRGEWGVALRHFRNASQVQPDAADIQYLIGEAQRMVGDYRAAINSYDVALSMNQG
                     FAPAYLGRALASQAIDPKRDVGDDLAKAVQYDPDFGLARLEKASYALAGGDFEAAQED
                     LEVAKQIMTDSPLLAFYLAELALQSGDRVAALEYARQAYELDRTLLPAYRLLGEMALA
                     NGEFDTAREVLEIYVQYAEEDARGWLAMGQAYMEFAGPEQAYRDLVQKARKRDVEAAM
                     QAFERAIELDKTLPGVYLYRAVTHLAMNEGQPAVNDLMMARRLDTNSFAINLGLGRAL
                     LVAERLDDARAQIDSSEKLAENDAQLAAIYYWRALAMEAGNLERAAAPVWEAMLNLAD
                     DSVPEEWRLVAMAHIAALTPTPTVTFTPTSSSTPTATPTRKPTLVGTSLPTSKVTSTP
                     TQ"
     gene            2147..>2783
                     /locus_tag="AC16V7_02735"
     CDS             2147..>2783
                     /locus_tag="AC16V7_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256064.1"
                     /translation="MEDRSDLITLLSQKSPQRPKIIRDINQPVWFRWLVSFVGFLLLL
                     VSTWFATTLQDHFWYILLIGLFVAFQVIFPLKLLSNEADLSHVITLGGGLLYGPVVAC
                     WAFTIGVALGYGIRWFESRKWNQSSIPPAVPVLDVLTLIGVHHIAMLVALPLFGLWQV
                     VSPIPPSSAVIWGEMGPALLLFALIYMFLYIGNTLVAGKKQYDFWRDLLYLA"
BASE COUNT          654 a          697 c          718 g          714 t
ORIGIN      
        1 gggtgatcaa gatgctcccc acgaagagcg agaaagcgtc gtagtcgatc aagcggagat
       61 cacattccaa atctcacaaa tgtagtacat ttggggacaa gtagatttta ttcacttaaa
      121 tcggttataa gcccatccag aatcctttca aacttgtatt gtccttatag cccctgccgt
      181 ccttgtctgc atagcgagcc tatgctaaac tcctttgtgg gaacaagcca tgcagcaaga
      241 ttacatgctc aaagaggcta tcgaagccgt ccgcgagggg tacaaagtcc gggcgcggga
      301 tttgcttact cgcctgctgc gagctgacca gacaaacccg gtatactggc tgtggatgag
      361 cagcgtggtg gagacatcac gcgagcaagt ttattgcctt aataacgtcc tgcgccttga
      421 cccacacaac gaagccgctc ggcgaggatt aaccctgatt ggcgccatgc cagctgatca
      481 ggatatgaac ccaacaccgc tggtgcgtcg ccagtggcat gtggctgtcc aggatgaacc
      541 gccacaaggc atcaaagcct tgtgggctaa ccccgtaata cgaatctcgt tttctggcac
      601 cctagcactt gtggttattg ccttgatttt tgtaggtatt tttggcttgg gagagaaacc
      661 aattgcagtt gctgcccgcc ccacgaaaac cccaggtccg cctcccacct tcacttctac
      721 accaacctat attggcagca cgcgtaccgc aaacaagatt accccaacac caccagtgac
      781 tggaccgctc ccgctttgga tgctgctcga tgcgacctac acgcccacgc cagtctacgt
      841 aaatacgcca cacccaatca gcgaggatta ccgcattgga cagcgtgcct tcgggcgggg
      901 tgagtggggg gtagccctgc gtcattttcg aaatgcgagc caggtgcaac ctgatgcagc
      961 agatattcaa tacttgatcg gtgaagcaca gcgcatggtt ggagattacc gcgctgcgat
     1021 taattcctac gatgttgcac taagtatgaa tcaaggcttt gcgccagcct accttgggcg
     1081 ggcgcttgcc agccaggcga tagatcccaa aagggatgtg ggcgatgacc tggcaaaagc
     1141 ggtgcaatat gatcctgatt ttggtcttgc tcgcctggag aaggcctctt atgctctggc
     1201 ggggggagat tttgaggctg cacaggaaga cctggaagta gctaaacaga tcatgaccga
     1261 ttcacccttg ctggcatttt acctcgctga gcttgccctt caatctggtg accgcgtcgc
     1321 cgcgctagaa tatgcccgcc aagcatatga attagaccgc accctgctac ctgcgtaccg
     1381 attgttggga gaaatggctt tagccaatgg tgagtttgac acggcgaggg aagtgctaga
     1441 aatttatgtc caatatgctg aggaggatgc gcgcgggtgg ctggcaatgg ggcaggcata
     1501 catggaattt gccggacctg agcaagcgta tcgtgacttg gttcagaagg cgcgaaagag
     1561 ggatgttgag gcagcaatgc aggcttttga gcgtgccatt gagttggata aaactttacc
     1621 tggtgtgtat ttgtaccgcg cagttactca ccttgctatg aatgaaggtc aaccagcggt
     1681 caacgacctg atgatggccc gccgcctgga taccaactca ttcgccatca acctcggttt
     1741 ggggcgtgcc ctcttggttg cagaacggct ggatgatgct cgcgcccaaa ttgacagttc
     1801 tgaaaaactg gcggagaatg acgcacagct cgcggctatt tattactggc gtgcattggc
     1861 aatggaagct gggaatctgg agagagctgc agctccggtt tgggaagcaa tgttaaattt
     1921 agcggatgac tcagtacccg aagagtggcg actagtcgcc atggcgcata ttgctgcctt
     1981 gaccccaact ccaacggtga ctttcactcc cacatcctct tccacaccaa cagccacgcc
     2041 gacacgaaaa cccacccttg ttggcacttc tttgccaaca agcaaggtga cgtcaacccc
     2101 tacccaataa acgaatgttc tgttttatca actttctgtg aatctcatgg aagaccgttc
     2161 ggacctgatt acactactca gtcaaaagtc gcctcagagg ccaaaaatca ttcgggatat
     2221 taatcaaccg gtttggtttc gctggcttgt atcctttgtt ggcttccttc tgttgttagt
     2281 aagtacttgg tttgcaacca cattgcaaga tcatttttgg tacatattgt taatcggttt
     2341 atttgtagct tttcaagtca ttttccccct gaaattattg tctaatgaag ctgatctaag
     2401 ccatgtgatt acattgggtg gggggttatt atatggaccg gtggtggctt gttgggcatt
     2461 tacgatcggc gtagccctgg gatatggcat tcgctggttt gaatcaagaa aatggaacca
     2521 atcttcaatt ccaccggcag ttcccgtatt ggatgttttg acgctgatcg gagttcacca
     2581 tattgccatg ctcgtggctt taccgttgtt tggtttgtgg caggttgtca gcccaattcc
     2641 gccttcatct gcagtaattt ggggcgagat gggcccggcg ttacttttat tcgcactgat
     2701 atacatgttc ctttacatcg gaaatacact ggttgcgggc aagaaacaat atgatttttg
     2761 gcgcgatttg ctttatcttg cct
//
LOCUS       DBNHOH010000152         1492 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1290724, whole genome shotgun sequence.
ACCESSION   DBNHOH010000152 DBNHOH010000000
VERSION     DBNHOH010000152.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1492)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1290724"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="AC16V7_02740"
     CDS             <1..1088
                     /locus_tag="AC16V7_02740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6256065.1"
                     /translation="RALARRGHQVTVFTTQYDGSLARDEMQHGVRVVRAPVALRISKG
                     VIAPAFGFMATRLVQEHDVVHLHLPQFDAPGVALRGRLLRKPTVLTYHCDLKLPPNPF
                     NYFLNRGVHLVHHMAAKLADRIVTYTQDYADNSPYLSNYKHKLQIIPPPVELPAVPHG
                     DVEAFAEAHNTSERRPVIGMAARLATEKGVEILLEALPSIIEKFPRAQVLFAGQYEDV
                     LGEREYYNRLMPVIRRYQQQGQWEFLGVLNPNQMAAFYPNLDVLVVPSLNSTEAFGLV
                     QIEAMMHGVPCVASALPGVRQPILTTGMGKVVPIGDSGALANAIQAILAEPDQYQKDP
                     RAIAQRFTPDAIALLYEEMFEDMLAKK"
     gene            1109..>1492
                     /locus_tag="AC16V7_02745"
     CDS             1109..>1492
                     /locus_tag="AC16V7_02745"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6256066.1"
                     /translation="MQEKDFLWLHLRDLPYFRALVRAVEAEYYQDIELPSPQLDIGCG
                     DGHFASLVFEKPVDVGVDPGGTAIRQAARLGSYRSLVQSDAGALPFPEGYFGSAFSNS
                     VLEHIPHVRDVLAETARVLKPGALFL"
BASE COUNT          342 a          373 c          402 g          375 t
ORIGIN      
        1 cacgcgcact tgccaggcgc ggccaccagg ttactgtttt taccacccag tacgatgggt
       61 ctttagcccg agatgaaatg cagcatggcg tgcgggtcgt tcgcgcccct gtcgcattac
      121 gcattagcaa aggggtgatt gcacccgcat ttggcttcat ggcgacgcgt ttggttcaag
      181 agcatgatgt ggttcacttg catttgccac agtttgacgc accgggagta gcgctgcgcg
      241 gtcgtctcct gcgcaagccc actgttttga cttatcattg cgatcttaaa ttgccaccca
      301 atccgttcaa ctattttcta aaccggggag tgcacctggt ccatcacatg gcggcaaagc
      361 tggcagatcg gatcgtgacc tacacccaag attacgccga caattctccc tacctatcaa
      421 attacaaaca taagctgcaa atcataccac cgccagtaga attacctgcg gttccgcatg
      481 gtgacgtcga ggcatttgca gaggctcaca atacatcaga gcgccggccg gttattggta
      541 tggcggctcg cctggcaaca gaaaaagggg tagaaattct cctggaggca ttaccgagca
      601 tcatcgaaaa atttccccgg gcacaggttc tctttgccgg gcagtatgaa gatgtattgg
      661 gtgagaggga atactataac cgcttgatgc cggtgatacg aagatatcag caacaaggtc
      721 agtgggagtt tttgggggtg ctgaacccca atcaaatggc ggctttttac ccgaatctgg
      781 atgtgctggt tgttccaagc ctgaattcga ctgaagcctt tggattggtg caaatcgaag
      841 cgatgatgca tggggttcct tgtgtggcct ccgcattacc tggcgtgcgg caacccatac
      901 ttaccaccgg tatgggcaag gtggtgccaa ttggagattc gggcgcatta gctaacgcta
      961 tacaagctat cctggccgaa ccggatcaat accagaagga tccgcgtgct attgcccaac
     1021 gatttacacc agatgctatc gccttattgt atgaagagat gtttgaggac atgctggcga
     1081 agaagtagaa aagtttattg gtcaatatat gcaagaaaag gattttctct ggctacacct
     1141 tcgcgatctc ccatacttcc gcgctctggt gcgggcagtt gaggctgagt attaccagga
     1201 tatcgaattg ccgtccccac aattggatat cggttgtgga gatggtcact tcgcctccct
     1261 ggtttttgaa aagccagtcg atgtaggggt tgaccctggg ggcaccgcga ttcgtcaggc
     1321 tgcccgcctc ggtagttatc gctcactagt tcaaagtgat gctggagcac tgccatttcc
     1381 agaaggttac ttcggtagtg cgtttagcaa ctcagttctt gagcatattc cgcatgtccg
     1441 agacgtactg gcggagacgg cgcgtgttct caagccaggt gcgctatttc tg
//
LOCUS       DBNHOH010000153         1772 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_393202, whole genome shotgun sequence.
ACCESSION   DBNHOH010000153 DBNHOH010000000
VERSION     DBNHOH010000153.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_393202"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="AC16V7_02750"
     CDS             complement(<1..220)
                     /locus_tag="AC16V7_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256067.1"
                     /translation="MTYGGYRQGYSGCGSNPCSIADSSNSLSADLAGAASHLLLYQGY
                     HNLIGRLYDYEVALINTEISMLKMVVMII"
     gene            163..858
                     /locus_tag="AC16V7_02755"
     CDS             163..858
                     /locus_tag="AC16V7_02755"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6256068.1"
                     /translation="MNRDSIHNQNNLADTRHRSSQSTQSHYDSLSRWYDLLTTWSERS
                     VRMAGLRMLDAQRGEAILEIGFGTGQAVLDLAKSVGDAGKVFGVDLSWGMVARARSKV
                     ASAGQSTRAALTHGDANKLPYKAEIFDAIFMSFTLELFNDEEIKAVLSECRRVLKKGG
                     RSCVIALLRKDHANIITRIYDWAHSHFPAAIDCRPIFVRNEILHAGFHIEQAVEMTLW
                     GLPVMLVIAKKTS"
     gene            1022..>1772
                     /locus_tag="AC16V7_02760"
     CDS             1022..>1772
                     /locus_tag="AC16V7_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HIR6256069.1"
                     /translation="MKPENYNIGFVSTRFRGHDGVSLEAEKWLEVLVRLGHNCYLFGG
                     DSDEYEKDYTFLVPEAHFKHPDIQEVYSIAFSALTRPPEVTRRIQTLKEHLKQRLNAF
                     VDQYKIRLLIVENALAIPLNIPLGLALTEFISETRIPVIAHHHDFFWERKRFLTNCVW
                     DYLNMSFPPHLPSIRHVVINSSASNQLSLRTGISAMTIPNVMDFDHPPQPPDDYTATF
                     RPDVGLNSGEYLFLQPTRVVQRKGIEHAIELI"
BASE COUNT          474 a          381 c          423 g          494 t
ORIGIN      
        1 gaataatcat tacaaccatt ttaagcatgg aaatttcagt attgatgagc gccacctcat
       61 aatcgtacaa acgaccgatc aaattatggt atccttgata gagaagcaga tgacttgctg
      121 cgcctgccag atctgcgcta agggagttgg atgaatcggc aattgaacag ggattcgatc
      181 cacaaccaga ataaccttgc cgatacccgc cataggtcaa gccaatccac ccagtctcat
      241 tacgatagtt taagcagatg gtatgacctg ttaactacat ggagtgagcg ttctgttcga
      301 atggcaggac tcaggatgct tgatgctcag cgcggcgaag ccatccttga gattggcttt
      361 ggaacaggtc aggctgtttt agatctagca aagtctgttg gagatgcggg caaagtgttc
      421 ggtgtggatc tatcttgggg catggttgca cgtgcccgga gtaaggtcgc aagtgcaggt
      481 caatccaccc gagctgcgct aacgcatggt gatgccaata aattgcctta caaggcagaa
      541 atattcgatg cgatatttat gagcttcact ctagaactat ttaatgacga ggaaattaaa
      601 gctgttttat ctgaatgtcg gcgagtctta aaaaaggggg ggcggagctg tgtcatcgct
      661 ttgttgagaa aggatcatgc gaatatcatt accagaatat atgattgggc acatagtcat
      721 tttccggctg ccatcgattg ccgacccata tttgttcgga acgagatttt gcacgccggg
      781 tttcacattg aacaggcagt cgagatgaca ttatggggtc tgccggttat gctagttatc
      841 gcaaagaaaa catcctagct gttcttgcaa gtatcgatgg aaaactcgac tcttccgaat
      901 tttggatagg ttctaaatga tggattatgt tctcgatttg atcaacaaca atttcgcaat
      961 caatatatct ttattcgttg tttggatacg acgatccccc aataaccatt gaggagagat
     1021 tatgaaacca gaaaactata acattggttt tgtatctacc cgatttcgcg ggcatgatgg
     1081 agtttcgctt gaggcagaga agtggttgga agttttggtg agactgggcc ataactgtta
     1141 tctgtttggt ggtgatagtg atgaatatga aaaagattat acttttttgg tcccagaggc
     1201 gcatttcaag catccggata ttcaagaagt ttactcgata gcgttttcgg ctctgacacg
     1261 cccacctgag gttacacggc ggattcaaac acttaaggaa cacctgaagc aacgcttgaa
     1321 tgcttttgtt gatcaatata agatccgtct gttgattgtt gagaacgcgc tagcaatccc
     1381 gctgaatata ccgcttggtc tggctctaac tgaatttatt tccgaaacgc gcatccctgt
     1441 gatcgcacac catcacgact ttttctggga gcgtaagagg tttttaacca actgcgtgtg
     1501 ggattacctg aatatgtcat tcccaccgca cctgccatcc atccgccacg tggtgataaa
     1561 ctcgtcagct tcaaatcaat tgagtctccg aactggtatt tctgcgatga ccatcccaaa
     1621 tgtgatggac tttgatcatc caccccagcc accagatgat tacacggcga ccttccggcc
     1681 agatgttggc ctgaattccg gcgagtatct ctttttacag cctacgcggg ttgtccaacg
     1741 taaaggaatt gagcatgcca tagagttaat tc
//
LOCUS       DBNHOH010000154         4555 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1482652, whole genome shotgun sequence.
ACCESSION   DBNHOH010000154 DBNHOH010000000
VERSION     DBNHOH010000154.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4555)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4555)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4555
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1482652"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="AC16V7_02765"
     CDS             <1..1093
                     /locus_tag="AC16V7_02765"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR6256070.1"
                     /translation="EAFLHAGLPYKLVGAQRFYGRREIKDVISYLRLVHNPNDEISLT
                     RVINTPARGVGNKTVLALRTLAKKQGLSPGVLLLELGRDPDAAGIEALPGRSAGPLAN
                     FGRNLVGWRDFANIATPLSLMDRILSDIDYQSYLDDGSDEGRDRWDNVMELRRLAAEF
                     QENGLEAFLEQVALVSDQDTLEERINVPTLLTLHAAKGLEFPVVFIVGMNDGTLPHSR
                     SFDDPEAMQEERRLLYVGITRAKDRLYLVYAQNRGLYGYSEPIEPSRYLDDIPVDLVD
                     ETQPTRSGRLHRPAPRTDRWQPQKASVQSSDQPRFHPGLRVMHNVWGEGMVLNSRIQD
                     DDEIVDIFFESTGLKRVVASLAKLEVMEK"
     gene            1133..1630
                     /gene="tsaE"
                     /locus_tag="AC16V7_02770"
     CDS             1133..1630
                     /gene="tsaE"
                     /locus_tag="AC16V7_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIR6256071.1"
                     /translation="MPILDPHSFEFISRSAEQTRRLGMRLGALLRAGDVICLVGDLGS
                     GKTTLVQGIASGWGSLDLVTSPTFVLVNVYSGPDAARLYHLDAYRLSSAREAEDLDLE
                     PMLETGLLVVEWAERIQEALPREHLWVTLRWIDNEQRDLVFAARGKRYQSVLASVRKQ
                     IFGIM"
     gene            1630..2298
                     /gene="tsaB"
                     /locus_tag="AC16V7_02775"
     CDS             1630..2298
                     /gene="tsaB"
                     /locus_tag="AC16V7_02775"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIR6256072.1"
                     /translation="MLLALDTSTRVVGLALYDGVQVLGETVWLSRDYHTVELAPAVAN
                     MLARSDIKISDLSALAVAIGPGSFTGLRIGLALAKGLALAHSLPLVGVPSLDILAAAQ
                     PIQEEVMAAVLRAGRGRLAVGWYQASDGGWRAKGQVEALTAQELNERIQEPTLVCGEL
                     EEDEQRLLARKRKNVILAPVAQSVRRPAWLAELGWKRWLAGRVDDPNTLSPIYLHYNE
                     PIPG"
     gene            2295..2852
                     /gene="rimI"
                     /locus_tag="AC16V7_02780"
     CDS             2295..2852
                     /gene="rimI"
                     /locus_tag="AC16V7_02780"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIR6256073.1"
                     /translation="MMAVRSKTSPIDPPQRSNLIHIRPMRQDDLDQVITIDRASFSLP
                     WPESSYRYELLENPHSLLMVAEAEAPDGEKQVVGAVVVWLLEDEAHIATLAIHPEYRG
                     RGISQELLATVLARSIRGGARIATLEVRANNQIAHALYRRFGFEVVGRRLRYYLDNNE
                     DALIMTADGLDERYLEWLESHGWRK"
     gene            2938..3567
                     /locus_tag="AC16V7_02785"
     CDS             2938..3567
                     /locus_tag="AC16V7_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HIR6256074.1"
                     /translation="MNSKEILAQVSQEVSACTQCVLHHSRKNAVPGEGPANAKIMFIG
                     EGPGFHENEQGRPFVGAAGKFLEDLLGKIGMQRTDVYITNVVKCRPPANRDPMAEELE
                     ACNEYLERQIQAINPRVIVTLGRFSMARFLSNAKISDVHGQAMRVRGRLIVPMYHPAA
                     ALHQGSLRPVIERDFALLPELIASAADAPEVEEPKPPEEKTQPRQLSLF"
     gene            3579..>4555
                     /locus_tag="AC16V7_02790"
     CDS             3579..>4555
                     /locus_tag="AC16V7_02790"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HIR6256075.1"
                     /translation="MSANIKHNLIDQIKERKAQVAILGLGYVGLPLAAVMAEAGFHVI
                     GVDPDQRKVEMVSRGESHVQDVPSEQVARLVKAGQLVATTDFSVLQNAQAVSICVPTP
                     LRKTGDPDLSFILDATAELARYIHPGMVVVLESTTYPGTTREILLPHLGNKKGLVVGE
                     NFFLAFSPERVDPGRKDWTTLNTPKVVGGITADCGEVASIWYSQALQSVVPVSSTEVA
                     EMAKLLENTFRMINIGLVNEMALMCNRLGVDVWEVIDAAATKPFGFMKFTPGPGLGGH
                     CIPIDPLYLSWKLRALKYTARFIELASEINTAMPRHVVDLVQDALNGQS"
BASE COUNT          965 a         1191 c         1393 g         1006 t
ORIGIN      
        1 ggaagccttc ctgcatgccg ggctgcccta caagctggtg ggagcgcaac gcttttatgg
       61 gcgtcgtgaa atcaaagatg tcatttcata cttgcgcttg gttcacaacc caaatgacga
      121 gatcagcctg acgcgggtga tcaatactcc ggcgcgcggt gtgggcaata agaccgtgct
      181 ggcgctccgc acgctggcta agaaacaggg actgtcccct ggcgttttgc tgctggagct
      241 ggggcgcgac ccagatgcgg caggcattga ggcgctcccc ggacggtcgg ctggccctct
      301 ggcgaatttc gggcgcaacc ttgtcggctg gcgtgatttc gctaacattg ccacaccctt
      361 gagcctgatg gaccgcattc tgagcgatat cgactaccaa tcctacctcg atgacggcag
      421 cgatgaaggc cgcgaccgtt gggataatgt gatggagttg cgccgcctgg ctgctgagtt
      481 tcaggaaaac gggctggaag ccttcttaga gcaggtggcc ctggtctccg accaggatac
      541 gctggaagaa cgcatcaatg ttcccaccct gctgaccctg catgccgcta aaggattgga
      601 gttcccggtg gtttttatcg tggggatgaa tgatggcacc ttgccgcaca gccgctcgtt
      661 tgatgatccg gaggccatgc aggaggagcg tcgccttcta tacgtgggga tcactcgcgc
      721 caaggatcgt ctgtacctgg tgtatgccca gaatcgcggt ttgtatggtt acagcgagcc
      781 aatcgagccg tcacgctacc tggacgatat ccccgttgat ctggtcgatg agacgcaacc
      841 gacgcgctca ggcagattac atcgcccggc tccacgaact gatcgctggc aaccccagaa
      901 agcctccgtg caatcgtcag accagccgcg ttttcaccct ggattgcgcg tgatgcacaa
      961 cgtctgggga gaaggcatgg tgctcaacag tcgcattcag gacgatgatg agattgtgga
     1021 tattttcttt gaaagcacgg ggctcaaacg tgttgtggct tccttagcca agctggaagt
     1081 catggagaaa taggcgcatc cagaattcgg caggacagat tataatcttc tcatgcccat
     1141 actcgatcca cattcctttg agtttatcag ccgcagcgca gagcaaaccc gtcgcctagg
     1201 tatgcgcctg ggtgctctat tgcgggcggg cgatgtaatc tgcctggtgg gtgatctcgg
     1261 ctcgggcaag accaccctgg tgcagggcat tgcctctgga tggggatcgt tagacttggt
     1321 aaccagccca acctttgtgc tggtgaacgt ttattccggg ccggatgcag cgcgcctgta
     1381 ccacctcgac gcataccgct tgagtagcgc tcgcgaagcc gaagatctgg atttggaacc
     1441 catgctggaa accgggctgt tggtggtgga atgggctgaa agaatccagg aggcgttacc
     1501 gcgcgagcat ctgtgggtga ccctgcgctg gattgataat gagcagcgcg atctggtctt
     1561 cgctgcacgg ggcaaacgtt accaatccgt gctggccagc gtgcgcaagc aaatatttgg
     1621 gattatgtga tgttattggc tttagatacc tcaacccgag tggtaggtct ggcgctgtat
     1681 gacggtgtcc aagtgttggg tgaaaccgtg tggttgagcc gtgattatca tactgtcgag
     1741 ttggcgccag cggtagccaa tatgctggcg cgctcagata tcaaaatctc ggatctgagt
     1801 gccctggcgg tagccatagg gccaggctcc ttcactgggc tgcgcattgg tttggcgctt
     1861 gccaagggat tggcgctggc gcacagcttg cccctggtgg gcgtgccttc cctggacata
     1921 ctggctgccg cccagccgat ccaagaagag gtgatggcgg ctgtcttgcg agccggacgg
     1981 gggcgactgg ccgttggctg gtaccaggca tctgatggtg gctggcgcgc caaagggcag
     2041 gttgaggcgc ttacagcaca agagttgaat gagcgcatcc aggaacctac gctggtttgc
     2101 ggcgagctgg aagaggatga acagcgcctg ctggcgcgca agcgcaagaa cgttatatta
     2161 gctccagtgg cccagtcagt gcggcgccct gcctggctgg cagagctggg ctggaagcgc
     2221 tggctggctg gcagagtgga cgaccccaac acactctcgc ccatctatct gcattacaac
     2281 gagccgatac cgggatgatg gctgttcgat cgaaaacttc gcccatcgat cctccccagc
     2341 ggtcaaatct catccatatt cgcccgatgc ggcaggacga cctagatcag gtgatcacca
     2401 tcgatcgggc atcgtttagc ttgccctggc cggagagctc ctaccgctac gaattgctgg
     2461 agaatcccca ttctctgctc atggtggcgg aggctgaggc gccggatggg gaaaagcaag
     2521 ttgtgggggc agtggtggtc tggttgcttg aggatgaagc ccacattgca acgcttgcga
     2581 tccatccaga gtaccgcggc agaggcatca gccaggagct tctggcgacc gtgttagcca
     2641 gatcgattcg gggaggcgcg cgaatagcta ccctggaagt gcgagcaaac aatcaaattg
     2701 ctcacgccct ctataggcgc tttggctttg aggttgtggg tcgccgcttg cgctattacc
     2761 tcgataacaa tgaggatgca ctgattatga cggctgatgg attggatgaa agataccttg
     2821 agtggctaga atcccatggg tggcgcaagt gaccttgatg aaggcagcta agcagctgta
     2881 tacttcagct cccggtagca aatggtaata gaggcactca attaaggagg tgttgttttg
     2941 aattctaaag agatcttagc gcaggtttca caggaggtgt ctgcctgcac ccaatgtgtt
     3001 ttgcaccatt ctcgcaagaa tgccgtacca ggtgagggtc cggcgaatgc caaaattatg
     3061 ttcatcggcg aaggcccggg tttccatgaa aacgagcagg gtcggccgtt tgtaggtgcg
     3121 gcgggaaaat tcctggaaga tctgctggga aagatcggca tgcagcgcac ggatgtctat
     3181 attaccaatg tggtcaagtg tcgccctcca gccaatcgtg accctatggc cgaagagctg
     3241 gaggcttgca acgaatacct ggagcgccaa atccaggcca tcaatccgag ggtcatcgtc
     3301 acattaggac gcttttccat ggcgcgtttt ctgtccaatg cgaagatcag cgatgtgcac
     3361 ggccaggcga tgcgtgtgcg cggccggttg attgttccga tgtaccatcc agccgcggcg
     3421 ctgcatcaag gctcgctcag accagttata gagcgtgatt tcgcattgct ccccgaactg
     3481 attgccagcg ccgcagacgc gcccgaagtg gaggagccta agccaccaga ggagaaaacg
     3541 cagccaagac aattgagtct tttttagcgt gaggaaggat gagcgcaaat attaaacaca
     3601 atctgatcga tcagataaag gaacgtaaag cgcaggtagc catcctgggg ctgggttacg
     3661 tcggcttgcc gctggcagct gtgatggctg aagcaggttt tcatgttatt ggtgtagatc
     3721 ctgaccagcg caaggtggag atggtcagtc gtggggagag ccatgtccag gatgtgccca
     3781 gcgagcaagt ggcgcgcctg gtgaaagcag gccagctggt tgccaccaca gatttctctg
     3841 tcttgcagaa tgctcaggcg gtcagcatct gtgtgcctac gccgctgcgc aagacgggcg
     3901 accccgacct ttcctttata ctggatgcta cggctgagct ggctaggtat attcaccccg
     3961 gaatggtggt cgtgcttgaa tcaaccacct atcctggtac cacgcgtgag atcctgctgc
     4021 cgcacctggg taacaaaaag ggtttggtgg tgggcgagaa tttcttcctg gctttctcac
     4081 cggagcgagt tgacccaggg cgcaaagact ggaccacgct gaacaccccc aaagtagtcg
     4141 gcgggatcac tgctgactgc ggcgaggtag cgtctatctg gtactcgcag gcattgcagt
     4201 cggttgtgcc tgtctcctct acggaggtag ctgagatggc caagctgctg gagaacacct
     4261 tccgcatgat taacattggc ctggtcaatg agatggccct gatgtgcaac cgcctgggtg
     4321 tggacgtatg ggaagtgatc gatgcggctg cgaccaagcc ttttggattc atgaagttca
     4381 cccctggacc agggctgggg ggtcactgca tcccgattga cccactctat ctttcgtgga
     4441 aactgcgcgc cctcaagtac actgcccgct ttattgagct ggcatctgag atcaacacag
     4501 ccatgccgcg tcacgtagtc gacttagtgc aggatgcact caatgggcag agtaa
//
LOCUS       DBNHOH010000155         2070 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_521782, whole genome shotgun sequence.
ACCESSION   DBNHOH010000155 DBNHOH010000000
VERSION     DBNHOH010000155.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_521782"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="AC16V7_02795"
     CDS             <1..267
                     /locus_tag="AC16V7_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR6256076.1"
                     /translation="TLKRTWEILSQVMQPGALNGLTKELIYIAVSIANNCDYCIHSHT
                     ASAFAKGMTEEQYQELLAVIGMAHQTNALATGMKVPIDPQYLNE"
     gene            602..1627
                     /locus_tag="AC16V7_02800"
     CDS             602..1627
                     /locus_tag="AC16V7_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007267452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HIR6256077.1"
                     /translation="MSTRHFIRGSLFTFIAIMLLASPVLAAKNGPGSPTGSGQVFLPN
                     PVAFLGDQSLTDNKDANYPELEPAYQMVTLTNLDGSGYLIGDWANIRSETGPPAFSET
                     NTFIYTRDDDRFEQVMAYFWITEAQKYIQSLGFGSTLRPINMESQDVRINQWGVDNSY
                     QWDKHDLLRFGKGGVDDAEDAEVILHEYGHAMQGDQNPALIYAGESGAIMEGFGDYWA
                     VTVSELIAPSGDPACVADWDSVSYTASPPHCLRRIDRDYHYPEDLNGRIHHDGQIWSQ
                     ALWDIRSALGNVTADTLILEAQFDFAADTSMPAAAQVTVDTAQRLYGKRTAKIVLGAF
                     QARGILP"
     gene            1769..>2070
                     /locus_tag="AC16V7_02805"
     CDS             1769..>2070
                     /locus_tag="AC16V7_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6256078.1"
                     /translation="MSSDSYPPNPHSNSTQQTGKIRVLIVDDHAVVRQGLRTFLELQD
                     EATSLPITVVGEASNGSQAVDQAKLLQPDVVLLDLVMPEMDGVEATSRIIEKSPHSR"
BASE COUNT          535 a          545 c          532 g          458 t
ORIGIN      
        1 acattgaaac gcacttggga aatcctaagc caggtcatgc agccgggcgc gctgaatggt
       61 ctgactaagg aacttatcta tatcgctgtt tcgattgcaa ataattgtga ttactgcatc
      121 cattcccaca cagcctccgc atttgcgaaa ggcatgacgg aagaacagta tcaagagctg
      181 ctagcggtga tagggatggc tcaccagacc aatgccctgg cgaccggcat gaaagtcccg
      241 attgatccac aatatttgaa tgaataaagc ctggcagatc aggtctgctc ggcaacctgg
      301 ggtgggtaaa aagcactaaa caactcatac accactgcaa gtaggcgctt ggatggtaac
      361 agacgttcag gtacaaatac cgcgcacgta ggttgactca gcatcgcgaa ttatgctaat
      421 ttatcagcga aatagaatgt gggatttcac agaagaaact caaccaattt tgagttgacc
      481 gctttgagct tgatatttct ccgctttagc tcttttatga acctgtttta ctcaaccgtt
      541 tggcgcactt tggagtaagt atagtgtaga aaaattcaac caatatgttt aaggaggaaa
      601 tatgtccact cgacacttca tacggggctc tctgtttacc ttcatcgcca tcatgctgct
      661 cgcctcgcct gtgctggcgg ctaagaatgg cccgggctcg cccactggca gcggacaggt
      721 gttcttgccc aatccggtag cttttttggg cgaccaatcc ctgaccgata ataaagacgc
      781 caactacccc gaactcgagc cagcctacca gatggtcaca ctgaccaatc tagatggcag
      841 cggctacctg atcggcgact gggcgaatat ccgcagtgag accggtccac ccgctttctc
      901 agaaaccaac acatttatct atacccgcga tgacgatcgc tttgagcaag tgatggctta
      961 tttttggatc accgaggcgc agaaatatat ccagagccta gggtttggca gcacgctgcg
     1021 cccgatcaac atggagtccc aggacgtgcg catcaaccag tggggggtag ataattccta
     1081 ccaatgggac aaacacgact tactgcgctt cggcaaaggc ggggtggatg atgccgaaga
     1141 tgccgaagtg atcttgcatg agtacgggca tgccatgcag ggagaccaga atcccgcttt
     1201 gatatacgcc ggcgagtcgg gggcgattat ggagggattc ggtgattact gggcggtcac
     1261 agtgagcgaa ttgattgcgc catcgggtga tccggcttgc gtggctgact gggattcagt
     1321 ctcgtacacg gctagcccac cgcactgcct gcgccgcatc gaccgagact accactaccc
     1381 agaggatctc aatggacgaa ttcatcacga tgggcaaatc tggtcacaag cactgtggga
     1441 catccgcagc gccctaggga atgtgaccgc agacacgctg atccttgaag cccaattcga
     1501 cttcgcggca gatacctcaa tgcctgccgc ggcccaagtc actgtggata cggctcagag
     1561 attatacggc aagcgcacag cgaaaatcgt actgggggct tttcaggcgc gcggcatttt
     1621 gccgtagtag agtgataata aggagggtgg gtaggctcac tcaccctcca catccagccc
     1681 aaccaagggg ttattcccaa gagccatttt tactctatcc aaaggataga gtaaaattct
     1741 tttaaagact cacctaggag agcagcccat gagcagcgat tcgtatcctc caaatccaca
     1801 ttcaaattca acacaacaaa caggcaaaat tcgtgtcctc atcgtggatg accatgctgt
     1861 cgtgcgacag gggctgcgca ccttcctcga gctacaggat gaggctacat cgctcccgat
     1921 aacagtggtt ggagaggctt caaacggaag ccaggcggtg gatcaagcca agttgctcca
     1981 acccgatgtt gtcctgctcg atcttgtcat gccagaaatg gacggagtcg aggccacctc
     2041 ccgcatcatc gaaaaaagcc ctcattcgcg
//
LOCUS       DBNHOH010000156         4141 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1419444, whole genome shotgun sequence.
ACCESSION   DBNHOH010000156 DBNHOH010000000
VERSION     DBNHOH010000156.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1419444"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="AC16V7_02810"
     CDS             complement(<1..194)
                     /locus_tag="AC16V7_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR6256079.1"
                     /translation="MSNLIRRDPFREMMSLRSAMDRLFDSAFFGTQWEWQSLSGEIAL
                     DVAETENEFLVKASLPGINP"
     gene            590..2062
                     /locus_tag="AC16V7_02815"
     CDS             590..2062
                     /locus_tag="AC16V7_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256080.1"
                     /translation="MAYNVAEIKLSEGTRSLIGAFCLGVTLLVISRLIFKDWYKAAAV
                     ASLALILFSSYGHFYNYLEPISLAGLAVGRHRVLVPVYMALFGLGIWWITRSMVDIKV
                     LTRALNVVSITALIIPVLQISIYAVREASAEIVEPSTGTQQLSYSESKSPPDIYYIIL
                     DGYSRDDVLKNFYGYDNSSFLDELTASGFYIARCSQSNYSQTQLSLSSALNLNYLSEL
                     GQGFTEDRTDRVGMPGLIKHSAVRQLLEDLGYTMVAFETGYYWTQVEDAAVYISSKSS
                     VTNLLDITGGLNGFESLLIKTSAGLILADGATILPRFMRADLDHPQEIHRQRVLFTLD
                     QLGKLANMPGPKFVFAHIVSPHRPFVFGPQGESVDHEKEPIIGYRDQVAYLNSRMVPI
                     LQKIITDSATPPIIIIQGDHGGVDTSGKDRLAILNVYYLPDGGDQQLYENITPVNTFR
                     LIFNYYFGGQYDLLEDASYSSAYKTPFALTPIPNSRPGCP"
     gene            2118..3608
                     /locus_tag="AC16V7_02820"
     CDS             2118..3608
                     /locus_tag="AC16V7_02820"
                     /inference="COORDINATES: protein motif:HMM:NF053209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptA/MptB family glycosyltransferase"
                     /protein_id="HIR6256081.1"
                     /translation="MSEIIWSKLNQWGGITLLAGVGMTAAFVPLACLPTLRGQNVIVF
                     LSHFLLAGAAYIIAVSRLGRDRLPMRVLWAFAILFRLIFLTTSPTLSDDVYRYIWDGH
                     LLGERVNPYALEVNSPALDAYSIPQRAQVNHNWMASPYLPVAQLFFGSVTRIAPQSVL
                     AFQIASVVLDLFTGWLLVRILKQVALPRIHALIYLWNPLVVVEFSHGAHVDALMLFFI
                     TLALYFIVCVPDGPSRQWWLYTSAIALAAATLTKGLPLIVAPFYLRRWGWRRLIVYVT
                     ILVGVLASCAVGAGWGLFGALDGRGLFGALRIYLNGWSFNSGINYWLETGLSSFQQSG
                     RWPAWAVGADVVRLVRWLTSAAQILVVIVAGMVAWRWDGSPRGEPVERDRRLLRLCAI
                     PIGAYLLLTPTLHPWYVTLLLIFLPFLSPAQGEKMSVGRFIWPWVYFSIAVALSYLTY
                     IDPNNLREFAQVRFIEYIPLYLLLIWAAWPLIRHGKMFKRAVKRIS"
     gene            3632..4129
                     /locus_tag="AC16V7_02825"
     CDS             3632..4129
                     /locus_tag="AC16V7_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256082.1"
                     /translation="MLKSIFKKIDNIDSLTWIALLRMMVGLMFLSTWYSNQLKGFYTP
                     DGLLEFFTNVFPQSENPLGWYAAFIQGIILPIRNLFAPFQLVAEFLLGLALLVGGLTP
                     LFSLAGIFFLTNTFLATFGHDWPWAYMLPIGILGVVFLARAGRSLGVDAILLRRFGER
                     GRFLW"
BASE COUNT          868 a         1012 c         1074 g         1187 t
ORIGIN      
        1 tcagggttga tgccaggcaa ggaggctttg accaggaact cattctcagt ctcggctacg
       61 tccagggcta tttcaccgct tagtgattgc cactcccatt gcgtgccgaa gaaagcactg
      121 tcaaaaagcc ggtccatcgc actgcgcaaa gacatcatct ctcggaaggg atctctgcga
      181 attaagttgc tcatggttta atctccttta ctgataaatt atttatggtt tactattgtt
      241 tggtttcctt gcaatatcat cttacccaat gggtataaga aaaagattgg agttatatat
      301 gaggtttgta aatctatgca cttgctggtg ggaaatagag gggattgagg ctcgtcaatc
      361 cgttgccctt agtttggact ataatatttc acctacccaa ccttcggcct gctgagcggc
      421 ctcaccctcc gataccaatt agagcagctg accttgggta tttagtctca ataaggtgcc
      481 cgcctcttac tgtattgggc aattgtttat gaagcgacca gataaattac gaatcaatca
      541 aattccatgg caccctgtta tcattgcgct ttttccggtg ttgactctat tggcttataa
      601 tgttgcagaa attaagctat ctgaagggac acgctccttg atcggggcat tttgcctggg
      661 cgtcacactt ctcgttatct ccagattgat tttcaaggat tggtataaag cagcagcagt
      721 tgcttcgctg gcgctgatac tgttctcctc ctatggtcac ttctataatt acctggagcc
      781 aatcagcctg gctgggcttg cagttggccg gcaccgcgtt ctggtccctg tctatatggc
      841 tttattcggg ttgggaatat ggtggataac tcgatcaatg gttgacatca aggttctgac
      901 acgcgctctg aatgtcgtct cgatcactgc gttaatcatc cctgtattgc aaatctctat
      961 ttatgctgtt cgagaggcga gcgctgagat tgtcgaacca tctactggca cgcagcagtt
     1021 atcctattca gaaagtaaat ctcccccaga tatttactac atcatcctgg atggctattc
     1081 gcgtgacgac gtcctgaaaa acttctatgg ttacgataac agctccttcc tggatgaatt
     1141 gactgcatct ggtttttaca ttgctcgctg cagccagagc aactattccc agacccagct
     1201 ttctctctct tctgcgctga acctgaatta tctatctgaa ttgggccagg gctttacgga
     1261 agaccggacc gatcgggtgg ggatgcccgg tttgatcaag cacagcgcgg ttcgacaatt
     1321 gctcgaagat cttgggtaca ccatggtggc ctttgaaacc ggttactatt ggacccaggt
     1381 ggaggacgcg gccgtctaca tttcatcaaa atccagcgtg acgaacctgt tggatattac
     1441 cgggggtttg aatggttttg aatccttgct catcaagact tccgccgggc ttattctggc
     1501 agatggagca acgatcttgc ccagatttat gcgcgccgac ctagatcatc cccaggagat
     1561 ccatcgccag agagtgctgt tcaccctcga ccaacttggc aagctggcaa acatgccagg
     1621 gccgaaattc gtctttgccc atatcgtatc cccccatcgg ccctttgtat ttggcccaca
     1681 gggcgagagt gtggatcacg agaaggaacc catcattgga taccgcgatc aggttgccta
     1741 tctgaattcg cgcatggtcc ccatactaca gaaaattatc actgattctg ctacaccgcc
     1801 aattatcatt attcaaggtg accatggagg cgtggataca tcgggcaagg accgcttggc
     1861 gatcttgaat gtgtactacc tgccagacgg aggcgaccag caattgtatg aaaacatcac
     1921 ccctgtcaac accttccgcc tgatcttcaa ttactatttt gggggccagt atgatctcct
     1981 cgaagatgcc agctactctt ctgcttacaa gactcctttt gcattaactc ctatcccgaa
     2041 ttcgcgtcct ggctgtccgt agagctcgca cacaataaaa tgccgggaat atgcaggtta
     2101 gttggctacc agatactgtg agcgaaatta tttggagtaa attaaaccag tggggtggaa
     2161 ttacgctcct ggctggggtt gggatgacag ctgcttttgt gccgcttgcc tgcctcccca
     2221 cgctgagggg ccagaatgtt attgtcttcc tgagccattt cttgctggcg ggcgcagctt
     2281 acatcattgc agtgagtcga ttgggccgcg acaggctccc gatgagggtg ctatgggctt
     2341 ttgccatcct gttccggctg attttcttga ccacctcgcc caccctctcc gacgacgttt
     2401 atcgatatat atgggatggt cacttgttag gagagagggt aaacccctat gcactagagg
     2461 tgaattcacc cgccttggac gcctattcca ttccacagcg agcccaggtc aatcacaatt
     2521 ggatggcctc cccctatcta ccagttgctc agttattttt tggatcggtt accaggattg
     2581 cgccgcagag cgtgctggca tttcagatcg cttcggttgt gctagaccta tttacaggct
     2641 ggttgttggt gcgaattctg aaacaggtcg cgctcccaag gatacacgcc ttgatctatt
     2701 tgtggaatcc actggttgta gtggagtttt ctcatggggc gcatgtggat gcattgatgc
     2761 tcttttttat tacgcttgcg ctttatttca tcgtttgtgt gcctgatggt ccgtcgagac
     2821 aatggtggtt gtacacatct gcaattgccc tggcggctgc gacgttaacc aaaggcttgc
     2881 cccttattgt ggcgcctttc tatttgcgcc gttggggctg gcgaaggctg atcgtctatg
     2941 tcacaatcct ggttggagta ttggcgtcgt gtgctgttgg ggcaggatgg gggctatttg
     3001 gcgcgctgga tggtcgggga ctgtttggcg ctcttcgcat ctacttgaac gggtggagct
     3061 ttaatagcgg gattaattat tggctggaaa ccgggttgag cagtttccag cagagcggca
     3121 ggtggcctgc ctgggctgtg ggagcggatg tggtccgcct ggtaaggtgg ttgacgagtg
     3181 ctgcacagat ccttgtggtg atagtggctg ggatggtcgc ctggcggtgg gatggttcgc
     3241 ctcggggtga gccagttgaa agggatcgca ggctgctccg tctgtgcgcg atcccaatcg
     3301 gtgcctacct gttgttaacc ccgaccctac atccctggta tgtgaccctg ctcctcattt
     3361 tcctcccctt cttgtcgccc gcccaaggag aaaagatgtc tgtggggcga tttatatggc
     3421 cttgggttta tttttcgatt gctgtagcat tgtcctacct tacgtatatt gacccgaata
     3481 acctgcgcga atttgcccag gtgcgtttca tcgaatacat ccccttatac ctgctattga
     3541 tttgggcagc ctggcctttg atccggcatg gtaagatgtt taagcgcgca gtaaaacgga
     3601 tcagctagag ggaatattcc caggagaaga aatgctaaaa agtattttta agaaaataga
     3661 caacatcgat tcgctaactt ggattgcatt attgcgcatg atggtgggtc tcatgttcct
     3721 gagcacttgg tatagcaacc agctcaaagg tttttacacg ccggatgggc tgttggagtt
     3781 cttcactaac gtcttcccgc aatcggaaaa tccgctaggc tggtatgctg cctttatcca
     3841 agggatcatt ctgccaatcc gtaacttgtt cgcccctttt cagttggtcg cagagttcct
     3901 cttaggattg gcgctgctgg tgggagggct gacgccccta ttcagcctgg cagggatttt
     3961 cttcctgacc aataccttcc tggctacctt tggtcacgac tggccctggg cgtatatgct
     4021 gccgattggc attcttgggg tggtatttct cgcgcgtgca gggcgttccc tgggtgtgga
     4081 tgctatcttg ctgagacgat ttggagagcg cgggcggttt ctttggtaat gcaagacagt
     4141 t
//
LOCUS       DBNHOH010000157         3393 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1482811, whole genome shotgun sequence.
ACCESSION   DBNHOH010000157 DBNHOH010000000
VERSION     DBNHOH010000157.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1482811"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..625)
                     /locus_tag="AC16V7_02830"
     CDS             complement(41..625)
                     /locus_tag="AC16V7_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256083.1"
                     /translation="MPRQFRNIILLMAFLGLVQGSAACDSTQATPQPIPGATSTPTQI
                     LSPQIEIVTEEPQIDTPVPIMEEESDLDPYVKVEVSEINLNLGETVSVTGWPVDINLP
                     NYYLILRDEGIQDNPFTAKVTHDNQVTPGEGRSKVVEWVSAEGKVDQVIFVLRAIGEG
                     VTAMDIEAIGELQLQDGSTLSVAGSGSVLIIVGD"
     gene            complement(629..2524)
                     /locus_tag="AC16V7_02835"
     CDS             complement(629..2524)
                     /locus_tag="AC16V7_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256084.1"
                     /translation="MDKSGDLALLRRFEPVLRFTRGERFFPMDVERYIGQCSLWMQQE
                     GQEPELLVPQGELTLDKLVEPRQIGFGAVHFLKFIEPLDILELARYSFDRAVSSIRQA
                     LKLSLQDPLHKFRAGRGRLARVGYGSRFVDALFSLTLMLRGRVPGDTAAAAALAYKRM
                     QDETERYCYYGRVARQNGWVILQYWFFYPFNNWRSGFFGVNDHEGDWEMISIYCSETH
                     SDGAELPSVDRLVPEWVAYASHDFSGDDLRRRWDDPEILKVPDAQGNLHPVVFAGAGS
                     HASYFSQGEYLTELELPFLSALVQLVDKLQSLWVKVLHQSSGSASSPGFNVFRIPFVD
                     YARGDGLALGPGQDREWQPEVISESTAWAISYRGLWGLYARDPIAGENAPAGPMYNRD
                     GSVRQSWCNPLGWSGLDKLPPNHDAPEVLKRKLDEIQAYQVSLKTKIDQQSSKLVGMG
                     VEAAAMQGYPHLEQAHRAQEEQIATLSAEVHHLRQELAQVDARLDALRLYGEQLSQAD
                     PGPMRAHIHRAHHPSPESELRLASVAEFVAAVSIGALMVGIVLLIVFARHYLLIGLAA
                     MIGVLIFIEAGFRRQLHRLINSLTVGLAIVSAMVLLFEFFWQIVVIGVLAAGLYIMWE
                     NLREITG"
     gene            complement(2524..2907)
                     /locus_tag="AC16V7_02840"
     CDS             complement(2524..2907)
                     /locus_tag="AC16V7_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256085.1"
                     /translation="MIIKIFQGLGLLGFGVFQASQFGWGWEGSELVFTPLVPYSVIDL
                     AYSGLLYLILAIITLFIALELWLVRRWAWLAAMTLQGLSLLAALFGYLQGQPNYISMA
                     LGIVLVLYLNQAEVQAVFRRSPKSA"
     gene            complement(3005..>3393)
                     /locus_tag="AC16V7_02845"
     CDS             complement(3005..>3393)
                     /locus_tag="AC16V7_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR6256086.1"
                     /translation="ISPDFVSDEAREAVQHVLARMPSGYYNTDLGNSLENFSQDYLDT
                     VDGRTTFLVVGDGRNNYNDPRVDIFSTLARRSRRTIWITPEAPGLWGSGDSDMLKYAP
                     SCDVILKAGNLAELTTAVDKLLAPHT"
BASE COUNT          897 a          952 c          757 g          787 t
ORIGIN      
        1 ttgaattact tgtactctcg cttgaaatag ggataaataa tcagtcccca acaatgatta
       61 atactgatcc cgaacccgcc acactcaggg tcgagccatc ttgaagttgc aattcaccga
      121 tagcctcaat gtccatggca gttaccccct cgccgatggc gcgcaggacg aatatcacct
      181 gatccacctt accctcggca gagacccatt ccaccacctt acttctccct tcgccaggtg
      241 taacctggtt atcgtgcgtc accttggcgg tgaaaggatt atcttgaatg ccttcgtcgc
      301 gcaaaatcaa ataataattt ggtaaattga tatcaactgg ccagccagtg accgatactg
      361 tttccccaag gttcagatta atttcactga cctccacctt cacataaggg tctaaatcgc
      421 tctcctcctc cattatcggg acgggggtat cgatttgtgg ctcctcagtt acgatctcaa
      481 tttggggaga cagtatctgc gtgggggtac tggttgcgcc aggtatgggc tggggagtgg
      541 cctgtgtaga atcacacgcc gcagaaccct ggactaaccc aagaaatgcc attaaaagta
      601 tgatgttcct gaactgtcga ggcataaatt atcccgtaat ctcacgcaga ttttcccaca
      661 taatatacaa gcccgccgcc aatacgccaa tcacaacaat ctgccaaaag aactcaaata
      721 gcaagaccat tgccgaaacg atcgctagtc ccacagtcag gctgttaatc aggcggtgta
      781 attgacggcg gaagcctgct tcaataaaga taagcacgcc aatcatcgcg gccagtccaa
      841 tcaatagata atggcgagcg aacacaatca agagcacaat accgaccatc aatgcaccga
      901 tgctcactgc agcgacaaac tctgccacac tggccagtcg cagctcactt tcaggcgaag
      961 ggtgatgagc acggtgaata tgagcccgca tcgggccggg atcagcctgg cttaactgct
     1021 caccatacag cctgagggca tccagtcggg catccacctg agccagctcc tggcgtagat
     1081 gatgcacttc tgctgacaac gtcgctatct gctcttcttg ggcgcggtgt gcttgctcta
     1141 gatgaggata accctgcata gccgccgctt caacccccat ccccactagc ttggaactct
     1201 gctggtcaat tttggttttt aggctcacct gataggcttg aatttcatca agcttgcgct
     1261 tcaaaacttc tggcgcatca tggttcgggg gtaatttatc tagtccagac cagcccaggg
     1321 gattgcacca gctctgcctt acgcttccat cgcgattgta catcggcccc gccggtgcgt
     1381 tttcaccagc aattggatcc cttgcgtata aaccccaaag tccccgataa gaaattgccc
     1441 aggctgtgct ctcgcttatc acctctggct gccactctcg atcctgccca gggccaagcg
     1501 ccaaaccatc accgcgggca taatccacga atgggattct aaagacatta aaaccaggcg
     1561 atgatgcact gccactcgat tggtgtagga ccttcaccca caaactttgt aacttatcca
     1621 caagttgaac caatgcagag agaaaaggaa gttcaagctc cgttaaatac tcaccctggc
     1681 taaaataact ggcatgactg cccgccccag caaaaactac cgggtgtaga ttgccctggg
     1741 cgtcgggtac cttcaatatc tctggatcat cccagcgccg gcgcaaatcg tcgccagaga
     1801 aatcgtggga cgcatatgcc acccactctg gcaccaagcg gtcaactgat ggcaactctg
     1861 cgccatcaga gtgggtctcc gagcaataaa tgctaatcat ttcccagtcg ccctcgtggt
     1921 cgtttacgcc aaaaaagcca gaacgccagt tattgaaagg ataaaagaac caatactgca
     1981 atatcaccca accgttctga cgagcgacac gaccataata acagtagcgc tcagtctcat
     2041 cttgcatgcg cttataagcg agcgctgctg cagcagccgt atctcctggg acccggccgc
     2101 gcagcatcag cgtcagggag aaaagtgcat ccacaaagcg cgagccatag ccgacacgtg
     2161 ccaatcgccc cctgcccgcg cgaaatttat gcaggggatc ttgcaagctg agttttaatg
     2221 cctgtctgat tgaagagacc gcccgatcga aggaatagcg tgctagctcc agaatatcaa
     2281 gaggttcaat aaactttagg aaatggaccg ctccaaatcc aatttggcgc ggctctacca
     2341 atttatcaag agttaattca ccttgaggga ctaatagctc aggttcttgg ccttcttgtt
     2401 gcatccacaa gctacattgg ccgatatatc gctctacatc catcgggaaa aaccgctcac
     2461 cccgagtaaa gcgaagcacc ggctcgaagc gacgcaataa tgctaggtct ccagatttat
     2521 ccattaggca cttttcggtg aacggcgaaa taccgcctgt acttcagctt ggtttagata
     2581 cagcacgagc actatcccaa gggccatact tatatagttc ggctgccctt gaagataccc
     2641 aaatagcgca gccaacaagc tgagaccttg cagcgtcatg gcagccagcc atgcccatct
     2701 gcgtaccaac cacaattcta gagcgataaa tagcgtgatg atcgctaaaa tcaaatagag
     2761 caaaccagaa taagcaaggt caattaccga atatggcaca agaggcgtaa atacaagttc
     2821 gctgccttcc catccccatc caaattgact tgcttggaat acaccaaaac cgagtagccc
     2881 caggccttga aaaatcttta tgatcacaag tattcccagg ctacgaggtc gctcaacgat
     2941 ttttcttttc tcgagtttct tgagtcccat tggaagattg tatcaggcga agttgaccta
     3001 tttattatgt atgtggagca agcagtttat ccaccgccgt ggtcagttcc gctaagttgc
     3061 cagccttcag gattacatca caggagggag cgtatttcag catatcgcta tccccactgc
     3121 cccacagacc aggtgcctct ggcgtaatcc agattgttcg cctgctgcga cgggctagcg
     3181 tgctgaagat atccaccctg ggatcattgt agttattgcg cccatctccc accaccagaa
     3241 atgtggtgcg cccatccacg gtatcaagat agtcttggct gaaattttcc agactgttgc
     3301 caagatctgt attgtaataa cctgacggca tgcgcgctaa tacatgctgt acagcttcac
     3361 gagcctcgtc acttacaaaa tctggtgaga tat
//
LOCUS       DBNHOH010000158         3804 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_9227, whole genome shotgun sequence.
ACCESSION   DBNHOH010000158 DBNHOH010000000
VERSION     DBNHOH010000158.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_9227"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="AC16V7_02850"
     CDS             complement(<1..753)
                     /locus_tag="AC16V7_02850"
                     /inference="COORDINATES: protein motif:HMM:NF015649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR6256087.1"
                     /translation="MLEVWLLGKFEVRLDGVPVHLPSRSEQSLLAYLLLNAGTAYRRE
                     KLAGLFWPESDESNARGYLRIALWRIRKAFKDLSPHSPDFFVTDKLTIAFNCELECWS
                     DVAVLKNGDGQSIQGLVEATSVYQGELLPGFYDEWVVLERESLRAAFERKIYTLVERL
                     LSERRWQEAIEQTERWISLGDTPEPAYRGLMIAHAGLGEASAAIAAYQRCVEALKKDL
                     DIQPSTETRALYEKIRSGQEIYPALRSRHIRGF"
     gene            complement(1186..2442)
                     /locus_tag="AC16V7_02855"
     CDS             complement(1186..2442)
                     /locus_tag="AC16V7_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256088.1"
                     /translation="MRLDRIASIVAFLALVALLLGGSSLPAGDKIERVRAFTREIEFD
                     YVAWTLNALEVKLSQVALGTSAYLSNDARHQQVLDYLDLVVQIQQSEAQLEEFFASPE
                     IANPQEASAPLRQKLEEMYARRSQLGPLAESILQEQLSNTAAEIGITLGGQPIPPVLY
                     HSTPLPRALIVSPREVIRQDNNISINPDLTIDQRVTLEERVDQALNVSSLVVEIGGIG
                     VYPTMVMQTSDLNWMAEVVAHEWIHNFLTLRPLGLNYETSPELRTINETVASIAGKEI
                     GRILVARYYPELAPPPPPEAPAESTPPLLPVEPPPFDFRAEMRITRVTVDQLLAEGKI
                     EEAEDYMEQRRLIFWDNGYHIRKINQAYFAFYGAYADQPGGAAGEDPVGAAVRLLRSR
                     SATLAEFLNRVSWMYSYDQLQRAVGE"
     gene            2536..3486
                     /locus_tag="AC16V7_02860"
     CDS             2536..3486
                     /locus_tag="AC16V7_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6256089.1"
                     /translation="MNFLITGAAGFLGSALANQLGREGHQVRGLDDLSTGDPQALSPD
                     VLFTRGDVNDRPKLWTLLQDVDCVYHLAARVAVPESLLYPREYNAVNVGGTVSLMEAM
                     RDVGVRRVVFISSGAVYGDQGEQPLREDAIPNPRSPYAVSKLAAEYYVRTIGDQWGIE
                     TVCLRVFNAYGPGQHLPASHPPVIPYFLKQAVRGGTLVLHGDGTQTRDYVYVSDVVRC
                     MVSAATAPNINKMIINVGSGTETSVRELVRLVRKVVTGGKPELIFNPRTHPGVSRMCA
                     DLTLAREKLGFQPRVSLVDGLSMTLERDLRFKKEPTRPLR"
     gene            3490..>3804
                     /locus_tag="AC16V7_02865"
     CDS             3490..>3804
                     /locus_tag="AC16V7_02865"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012036110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HIR6256090.1"
                     /translation="MPDPEQLERPFFSRSTLQVARELLGMRLVRQERDVRLVGVISEV
                     EAYRGEEDQGCHARAGRTPRTRVMYGSPGHAYVYFTYGMHWMLNFVTEAEGYPAAVLI
                     RAI"
BASE COUNT          811 a         1009 c         1107 g          877 t
ORIGIN      
        1 aaaacctcgg atgtgtcgcg atcttagggc tgggtagatc tcctggccag agcggatttt
       61 ctcatacaag gccctggttt cagtcgatgg ctggatgtca aggtctttct tcaaggcttc
      121 gacgcagcgc tgataggcgg caatggcagc agatgcttcc cccaatccgg catgggcgat
      181 cattaagccc ctataggctg gctctggggt atcgcccaag gatatccatc gctctgtctg
      241 ctcaatcgcc tcctgccagc gtctctcgga gagcagcctt tctaccagag tatatatctt
      301 tcgctcaaag gctgctcgca ggctctccct ttctaacacc acccactcgt cataaaagcc
      361 ggggagcagc tcgccttggt acacgctcgt tgcctccacc agaccttgaa tcgactgccc
      421 gtcaccgttt ttcagaacgg caacgtctga ccagcattcg agttcgcagt tgaaggcgat
      481 ggttaatttg tcggttacga agaaatcggg agagtgggga gaaaggtcct tgaacgcctt
      541 tcgaattcgc cacaatgcaa tccggagata tcccctggcg ttcgactcat ctgattcagg
      601 ccagaacaag ccagccagct tttcgcgccg gtaggccgtc ccggcattaa gcagcaaata
      661 cgccagcaga gactgctcgg agcgtgatgg gagatgaaca gggacgccat ccagccgaac
      721 ttcaaatttg ccaagaagcc atacttcgag cacttgttcc tccaaacttt ataaaaattg
      781 gctgtcgagg ggatgatgac gtgaagctca tcgcgccata attaagaacg ctacttggag
      841 taattctatc atagttggga aaatcatacg atgaatatat ggcgataaat acgatctata
      901 tacgtcgttt atttatactc ttgaacagat atgagaaacc gaatctagag tatagatcga
      961 aattagtcag gtgaaatgag gttgatatgt ataacgatgt gacgcaaagg aactacggca
     1021 aagcccgcat ggagatcctt cagaaagagg ctcaaatcag gcgggcagtg gcggcttatg
     1081 aagccgggaa gctgagaaat ctcccccaat catcaaattg cagaagagtg gcttccagcg
     1141 cctcctgagc tggctgctcg ctggtttgag atagtgatgt gtggattatt cccccaccgc
     1201 tcgctgcaat tggtcgtatg aatacatcca cgagacgcgg ttgaggaatt cggctagggt
     1261 ggcgctccgg gagcgcaaca ggcgcaccgc cgcgcccacc ggatcttcgc cggcagcacc
     1321 gccgggctgg tctgcgtagg caccatagaa ggcgaaataa gcctggttga tcttgcgaat
     1381 atggtagcca ttatcccaga agatcaggcg gcgctgttcc atgtagtctt ctgcctcctc
     1441 aatcttcccc tctgccagca gctgatccac tgtcacgcgt gtgatgcgca tctcggcgcg
     1501 gaagtcgaag ggtggcggct ctacagggag aagcggaggt gtggattcgg ctggcgcttc
     1561 cggcggtggt ggcggggcta gctccgggta atagcgcgct accaatattc gcccgatctc
     1621 tttgccggca atggaggcga cagtttcgtt gatggttcgt aattctgggc tggtctcata
     1681 attcagcccg agtgggcgca gggtgaggaa gttgtggatc cactcgtgtg ctaccacctc
     1741 cgccatccag ttgagatcgc tggtctgcat gaccatggtt gggtagacac cgatgccgcc
     1801 gatctcaacc accagcgaag atacgttcaa ggcctggtca acgcgctctt ctagcgtcac
     1861 ccgctggtcg atggtcaggt cagggttgat agagatgttg ttatcctggc ggattacctc
     1921 gcgcggcgag acgatcaatg cgcgtggcaa cggggtgctg tgataaagga ccgggggtat
     1981 gggctgcccg ccaagcgtta tcccgatctc ggctgccgtg ttggaaagtt gttcctgtaa
     2041 gatcgactcg gctaagggac ccagctgtga gcggcgcgca tacatctctt ccagtttctg
     2101 tcgcagcggg gcgctggctt cttgggggtt ggcaatctct ggactcgcga agaattcctc
     2161 caattgtgct tcactttgct gaatttgcac aaccagatcc aggtaatcga gcacttgttg
     2221 gtggcgggcg tcgttggaca gataggctga ggtgcccagg gcgacctggc tcaactttac
     2281 ttccagcgcg tttagcgtcc aggcgacata atcgaattca atctccctgg tgaaagcgcg
     2341 cacgcgctcg atcttatccc cggcgggcag gctgctgccg ccaagcaaaa gtgctaccag
     2401 ggcaagaaag gctacgattg aagctatacg atccaagcgc atgggggcga tgataacgcc
     2461 atcatagcag tatgtcaatt atggtaaatt gggagagcag gcatccatgc ctttcatgct
     2521 ataatcaccc ccacgatgaa tttcctgatc accggcgcgg ccggattttt aggctcagcg
     2581 ctcgctaacc aattagggcg cgaaggtcac caggtgcgcg gcctcgacga tctttccaca
     2641 ggcgatccgc aggcgctctc cccggatgtg ctctttacgc gcggcgacgt gaacgaccgc
     2701 cccaagcttt ggacgctcct ccaggatgta gattgtgttt accatctagc cgcgcgtgtt
     2761 gcggtgcccg aatcgctgtt gtacccgcgc gaatataacg ctgtcaatgt gggtggcacg
     2821 gtcagtttga tggaggccat gcgcgatgtg ggcgtgcgcc gggtggtgtt catctcttca
     2881 ggcgcggtgt atggcgacca gggcgaacag cctctgcgcg aagacgccat ccccaacccg
     2941 cgctcacctt atgcagtttc taagctggcg gcggaatact acgtgcgcac gattggggac
     3001 cagtggggca tcgagacggt ctgcttgcgg gtcttcaatg cctacggccc gggtcagcat
     3061 ttgcctgctt cccatcctcc tgtaatcccg tatttcctta aacaggcggt gcgcggaggt
     3121 actttggtgt tgcatggcga cggcacccaa acgcgcgact atgtttacgt tagcgatgta
     3181 gtgcgctgca tggtatctgc ggcgactgca cccaatatca ataaaatgat catcaatgtg
     3241 ggtagcggca ctgagacgag tgtgcgcgag ctggtacggt tggtgaggaa agttgtcact
     3301 gggggaaagc cggagttaat ttttaaccct cgcacccacc cgggtgtctc gcgcatgtgc
     3361 gccgatttga ccctggcgcg tgaaaagctg ggcttccagc cacgcgtttc actggtcgat
     3421 ggactcagca tgactctgga gcgcgatctg cgttttaaga aagagcctac tcgacccctg
     3481 cgctaattta tgcccgaccc cgagcaatta gaacgacctt ttttctcccg aagcacctta
     3541 caagtggcgc gggaattgct tggcatgcgc ctggtacggc aggaaaggga cgtgcgtttg
     3601 gtgggtgtga ttagcgaggt agaagcttac cgtggtgaag aggatcaagg ctgccatgcg
     3661 cgcgctggcc ggactcccag aacgcgcgtg atgtacggtt ccccgggtca cgcttatgtg
     3721 tactttacct atgggatgca ctggatgctc aactttgtaa ctgaggctga gggttatccg
     3781 gcggcggtgc tgattcgtgc catc
//
LOCUS       DBNHOH010000159         1869 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_393687, whole genome shotgun sequence.
ACCESSION   DBNHOH010000159 DBNHOH010000000
VERSION     DBNHOH010000159.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_393687"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..561)
                     /locus_tag="AC16V7_02870"
     CDS             complement(7..561)
                     /locus_tag="AC16V7_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256091.1"
                     /translation="MCTKKQRLNWNCGWILILLALASTACASQTGDVSIAEHATAQEI
                     TPKFAETPRDNNVPEALVDKVDESSCKGKFPSLSTGFDIQQAPSMDEPPAKVPFRDSV
                     FGTCMMRLTDRFEDLTSEDPSQGLKNEYSRVQSFNADGSRVLVRSIDAYWYVYDAQIL
                     KPMEHIPLSVEPRWDAESQICSII"
     gene            850..>1869
                     /locus_tag="AC16V7_02875"
     CDS             850..>1869
                     /locus_tag="AC16V7_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013087.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HIR6256092.1"
                     /translation="MPVVPSIIERTILLTLNRGPGPMMDLAGGIAFHALSVAIKLKIF
                     EAIGDKSLPPSEIAHLISTDTRATGLLLDALEALGYVEGKQGNYSNTPMTLKWILETS
                     PNSLACMISGMEGALDRWKYLDETIRNGSPSKLAWEWLDQHPGSWADYQALMIGMAHS
                     CADEIVSRVKLPPTARKLIDLGGGHGLYSIGFCRKYPGLTATVFDWPQAKDIANDTIL
                     SEGMNERVKFVVGDLWKDDYGSGYDVALLFQIIHMYSPEKNIQLLTKVREALSPNGIL
                     VINDQVAVDAPSPLAKLFARLQSLELLNSVNGQTYPPVEIEGWLVEAGYSNPSTMLLR
                     NTPGFG"
BASE COUNT          499 a          416 c          451 g          503 t
ORIGIN      
        1 tttcatctat atgatagagc aaatctggga ttcagcatcc cagcgtggtt caaccgagag
       61 gggaatatgc tccatgggct tcaatatctg cgcgtcataa acataccaat atgcatcgat
      121 gctccgcacc aaaactcggc ttccgtcggc attaaaggat tgtacacggg agtactcgtt
      181 tttcaaaccc tgggatgggt cttccgaggt gagatcttca aatcggtcgg ttaaccgcat
      241 catacaagtg ccgaaaaccg aatctcgaaa tggcactttg gctgggggtt cgtccatact
      301 aggcgcttgc tggatatcga aaccggtact caggcttgga aatttaccct tacaactgct
      361 ctcgtctact ttatccacaa gagcctcggg tacattgttg tctcttggtg tctctgcgaa
      421 ctttggtgtg atttcttggg ctgttgcgtg ttccgcaatc gaaacatcac cagtctgcga
      481 agcacatgcc gtgcttgcaa gggccagtaa gatgagtatc catccacaat tccagttaag
      541 tctttgtttc ttggtacaca tgaaatcaat cagctgtggg atacagagaa ttcgtcatga
      601 tcaaaatgca aagacgattg gtcaatacta tcactatctt ctcctttagc acattctgat
      661 gcaattgtcc catactatca aggactctag cgggcatgtg ctatgtagtg aaccccaccc
      721 tacatcgtct gggtgtcgaa tcctagttga attgggaaga gaatttctat atactgttga
      781 tggtgataca gtcagacgag tattcgcctc aggctgccaa atcgagttat tagattttgg
      841 gagggaaaaa tgcctgtcgt acctagcatt attgaacgaa ctattctgtt gacgctcaac
      901 agaggcccag gtccgatgat ggatctggct ggtggaatag cttttcacgc gttgtccgtt
      961 gcgatcaaac tcaagatttt cgaggcgatt ggagacaaaa gccttccacc aagtgaaata
     1021 gcccatctga tttccacaga taccagggca accggcttgt tacttgatgc ccttgaagcc
     1081 ctcggctatg ttgaagggaa gcaaggaaat tactctaata cacccatgac actcaagtgg
     1141 attttagaaa cttccccaaa tagcctggct tgcatgattt ctggcatgga aggagcacta
     1201 gatcgctgga agtatttaga tgaaacgatc agaaacggaa gtccctcaaa gctagcgtgg
     1261 gaatggttag atcagcatcc tgggagttgg gctgattacc aggcattgat gattggtatg
     1321 gcacattcgt gtgctgatga aatcgtctcg cgggttaaat tgccaccaac agccagaaag
     1381 ctgatcgatc ttggcggagg ccatgggctt tacagcattg gattctgccg caagtatcct
     1441 ggtttgaccg ccacggtatt cgattggcca caagcaaagg atatagccaa tgacaccata
     1501 ctttcagaag ggatgaatga gcgcgtaaaa tttgtcgtgg gagatttgtg gaaagatgat
     1561 tacggttcag gatatgatgt tgcgctgtta tttcagatca tccacatgta ttctcctgaa
     1621 aagaatatcc aattgctaac aaaggtcagg gaagctttat cgcccaatgg tattttggtg
     1681 atcaacgatc aggtcgcagt agatgcacca agtcccttag caaaactgtt tgcccgcctc
     1741 caaagtcttg aattgcttaa ctcagtaaat ggtcaaacct atccaccagt ggaaattgag
     1801 ggatggctgg tcgaagcagg ctatagcaat ccatccacca tgttgctaag gaacactccg
     1861 ggatttggt
//
LOCUS       DBNHOH010000160         4953 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1034959, whole genome shotgun sequence.
ACCESSION   DBNHOH010000160 DBNHOH010000000
VERSION     DBNHOH010000160.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1034959"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="AC16V7_02880"
     CDS             <1..307
                     /locus_tag="AC16V7_02880"
                     /inference="COORDINATES: protein motif:HMM:NF021926.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HIR6256093.1"
                     /translation="ETVQLHLRDAGTATEDLEMLLAPQAFSDPPSGALLFSCNGRGTR
                     LYDHPNGDISVIQATLEGTPLAGFFCAGEIGPLGDDNFLHGHTASLVIFRPIVKQEG"
     gene            320..2572
                     /locus_tag="AC16V7_02885"
     CDS             320..2572
                     /locus_tag="AC16V7_02885"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6256094.1"
                     /translation="MQSFGFIELLAIAQTVVYLLVAVLALWRQKAQPHQALYALVSVL
                     WLAAQLALIQGWLPFLLDDFTARLPLYGLLLLAVTLLWFARAYLSVQEGSWGWRLIGL
                     AWIATLLLVDSNLVAVPEEMWFRGGLMLPRDNFIVGGIVLGWCFFTGAATLMTLLVYR
                     RTRQPLQRNRIRYLAAVLFLVFTGEAVFLFAYWSLGSLLHLSGVLVGAYATLNARLPS
                     FRRAVLRSLSYLITSGLALLFYLAALMLSYATLRATGEANLWLVGLVLAAALAVFFTP
                     LLGQAQKWLDRLVAGVSHDPALILRQYSQTISNILDLGLLAKVVTELIGQALDIPRGY
                     LFAVSHEKDSEGHNSYRLSEVMDEDGDEPKDIILAEASTLCEYLHHQRGPLTQSEIDS
                     SPRFAEITPDERGWLLDLQMELFVPIHSKDEWIGLLALGPKNSGEPFWDDDQSLLASL
                     ADQTAVALENARLVESLVRLNNDFRRAIAALDQANRNLERLDRTKTEFISVASHELRT
                     PLTLINGYSQVLMEEPSLAGNANYAKMISGIHSGAQRLQEVLESMLDMAKIDTRELQL
                     NLQTVHMGEIIESLRAEFDTALQERNQKFETVDLASLPSIQADREALRKVIYHLLVNA
                     IKYTPDGGKIAVSGRAVLPSKDTLIESGVEIIVSDTGIGIDPSLHDVIFTKFYQTSDL
                     ALHSSGKTKFKGAGSGLGLAIAKGIVDAHFGRIWVESEGYDETRCPGSQFHVFLPLRH
                     PEKTRPKAAI"
     gene            complement(2781..3401)
                     /locus_tag="AC16V7_02890"
     CDS             complement(2781..3401)
                     /locus_tag="AC16V7_02890"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR6256095.1"
                     /translation="MTKTVILPADDPVSIRRALEILGQGGLVAFPTDTVYGVGALVSK
                     AQAIAQLYVVKGRSDEKAIPILLSDIAQLALVALEVSESVRRLADRFWPGPLTLVLPK
                     QPTLPAEISSQRTIGVRMPDHPIALALMRQTGPLAVTSANLSGGENSVTALQVMAQLG
                     GLIPLILDGGKTPGGSPSTVVDCTAVEPRILRSGPISLDQILEALS"
     gene            complement(3398..4168)
                     /locus_tag="AC16V7_02895"
     CDS             complement(3398..4168)
                     /locus_tag="AC16V7_02895"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR6256096.1"
                     /translation="MLLTIDIGNTNITLGLYKGETLGPRWRLATNHERMPDEYGVQLL
                     GLLSHVGHQTNELTGACLASVVPPLTGRMVEACQRYVGLDPLVLDAGVKTGVRIRYED
                     PRAVGADRIADAVAVQRLYGGPACVVDFGTATTFDAISADGDYLGGAIAPGIGIAAEA
                     LVMRTAKLPRVDLQRPPSAIGRNTPHAMQSGLLFGYVSLVEGMVARFRKELGREMKVI
                     ATGGLAEVIARETAVLEIIAPWLTLDGLRIIWELNQSK"
     gene            complement(4243..>4953)
                     /gene="panC"
                     /locus_tag="AC16V7_02900"
     CDS             complement(4243..>4953)
                     /gene="panC"
                     /locus_tag="AC16V7_02900"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HIR6256097.1"
                     /translation="SECASVIVSIFVNPTQFSPSEDLASYPRDLERDLQLLEADGVDL
                     VWTPTTEVMYPNGFQTWITVDDVSQPLEGAMRPGHFRGVTTVVAKLFTAVQPDKAYFG
                     QKDAQQAVVIRQMTRDLNFPIDVVVCPIVREPDGLAMSSRNVYLNPAERKAATVLSRA
                     LFSARDAFLSGERDAQQLRDRITEMVNAEPLARLQYVSCAHPDAIVELQGSVTRALLS
                     MAVFVGKTRLIDNVEIRG"
BASE COUNT         1037 a         1363 c         1477 g         1076 t
ORIGIN      
        1 cgagaccgtc cagctacacc tgcgcgatgc tggaaccgct accgaagacc tggaaatgct
       61 gctcgcgccg caggcgtttt ccgatccgcc gagcggggcg ctgctttttt cgtgcaacgg
      121 gcgcggcacg cgcctgtacg accatccgaa cggggatatc tccgtgatcc aagcgactct
      181 ggaaggtacg cccctggctg gattcttctg cgctggagag atcggcccgc tcggtgacga
      241 taactttctg cacgggcaca ctgccagcct ggtgattttc cgccctattg tgaaacaaga
      301 gggataattt ctaaacaaca tgcaatcgtt tggctttata gagcttttag ctattgctca
      361 gacagtcgta tatttgttgg ttgccgtcct ggctctttgg aggcagaaag cccaacccca
      421 ccaggcgctt tatgccttgg tctctgtcct atggcttgcg gcgcaactgg ctttgattca
      481 aggctggttg cctttcttgc tggatgattt tacagcccgg ttgccgctgt atggactgct
      541 gttactggcg gtaacccttc tttggttcgc acgagcctac ctgagtgtcc aggaaggtag
      601 ctgggggtgg cgattaatcg ggctagcctg gatcgccaca ttgctcctgg tggatagtaa
      661 cctggtggca gtacccgagg aaatgtggtt tcggggcggg cttatgttgc cgcgcgacaa
      721 cttcattgtg gggggcatcg tgctgggatg gtgcttcttc actggtgcag caaccttgat
      781 gaccttgtta gtttaccggc gcacccgaca accactgcaa cgcaaccgta tacgctatct
      841 ggcagcagtt ttattcctgg tcttcacggg cgaggcggtt ttcctgttcg cttactggtc
      901 tcttggaagc ctgctgcatc tctcaggtgt actggtggga gcctatgcaa cacttaacgc
      961 gcgcttgcct tcatttcgac gggctgtgct gcgcagcttg agctacctga tcaccagcgg
     1021 gctagccttg ctcttttacc tagcggctct gatgctctcg tatgcgactc tccgggctac
     1081 aggggaggcc aatctttggc tggtcggcct ggtgctggct gccgccctgg cagtcttctt
     1141 cacccctctc cttggacagg cgcagaaatg gcttgaccgc ctggtcgctg gggtcagtca
     1201 cgacccggcg ctcatcctgc gccagtacag ccagaccatc agcaatatcc tagacctggg
     1261 gctgctcgcg aaggttgtga cggaattgat cggtcaggcg ttggacatcc cgcgcggtta
     1321 cctgtttgcg gtcagccatg agaaggattc cgaggggcat aacagttatc gtctgagcga
     1381 ggttatggac gaagatgggg atgagccgaa agatatcatc ctcgctgaag cgagcacgct
     1441 gtgcgaatat ttgcatcacc agcgcggccc actcacccaa agcgagatcg actcctcgcc
     1501 acgcttcgcc gagatcacgc ccgatgagcg cggttggctt ttggatctcc agatggagct
     1561 ctttgtcccg attcattcca aagatgaatg gatcggtttg ctggccttag ggcccaaaaa
     1621 ttcgggtgag cccttttggg atgatgatca atccttgctt gccagcttgg ctgaccagac
     1681 ggcggtggcg ctggagaacg ctcgcctggt ggaaagcctg gtacgcttga acaacgattt
     1741 tcgccgggcg attgctgccc tggatcaggc taaccgcaac ctggagcgat tggaccgcac
     1801 caagacagaa tttatcagcg tggcctctca cgagctgcgc acgcctctca cattaattaa
     1861 tggttatagc caggtgttga tggaagagcc atctctggct ggaaatgcca actacgccaa
     1921 gatgatctct ggcatccaca gcggagcgca gcgtctccag gaagttttag agagcatgct
     1981 ggatatggct aaaatcgaca cccgcgagct gcagctcaac ctgcaaactg ttcatatggg
     2041 cgaaataatc gaatcgttgc gtgcggagtt cgatacggca ttacaagaac gcaaccagaa
     2101 gttcgagacg gttgacctgg cttcactgcc gtctatccaa gccgatcgcg aggcgttgcg
     2161 taaggttatt tatcatttgc tggtcaatgc gattaagtac acccccgatg gcggtaagat
     2221 cgcagtcagc gggcgcgccg ttttaccgtc aaaggacacc ttaatcgaga gcggcgtgga
     2281 gattattgtc agtgacactg gaattgggat cgaccccagc ctgcacgatg taatctttac
     2341 caagttttac cagacaagcg atctggctct acactccagc gggaaaacta agttcaaagg
     2401 cgccgggtcg gggcttggat tggcaatcgc caaaggtatt gtggatgccc attttgggag
     2461 aatctgggtg gaaagcgaag ggtatgatga aactcgctgt cctggaagcc agttccacgt
     2521 ttttttgcct ttgcgccatc cggagaagac tcgcccgaaa gcggcaattt aataactcat
     2581 tcgggattca attcgagatc tttgttaaaa acaaactgcg cctgggagaa ggcctggcac
     2641 ctagctccga agcgcagttt tgagtgagga gaatgcaact accattatat acacatagct
     2701 aaaagagtca tgaagagaag ataaaagttt ggttagaaga tagaaaaagg gggatttgtc
     2761 cagtttctca gagctggaga tcaagacaaa gcttcaagta tttgatctaa actgatcggg
     2821 ccagaacgca ggatgcgcgg ctccaccgcg gtacagtcca ccactgttga agggctgccg
     2881 ccgggcgtct tgccaccatc caggatgagc gggatgagcc cgccgagctg cgccatcacc
     2941 tgcaaggccg tcacggagtt ttctccaccg gataggttgg cagacgtaac cgccagcgga
     3001 ccagtctggc gcatgagcgc cagcgcgatg ggatggtctg gcatgcgcac gccaatggtc
     3061 cgctgagaag agatctcagc cggcagcgtg ggctgttttg gcagcaccag tgtgagcggg
     3121 ccgggccaga agcggtctgc cagtcggcga acgctctcgc tcacttcgag cgctaccagc
     3181 gccagttgtg cgatgtcgct gagcaggatg gggatggctt tctcatcgct gcgccctttc
     3241 accacgtaca attgcgcgat ggcctgcgcc ttgctcacca atgcgcctac gccgtagacc
     3301 gtgtcagtgg ggaatgctac cagaccccct tgccccaata tttcaagcgc ccgcctgata
     3361 gaaaccgggt catcggcggg gaggataacg gtctttgtca tttcgattgg ttcagctccc
     3421 agataatccg cagaccgtcc aaggtgagcc agggagcgat gatctcgagc acagcggttt
     3481 cgcgagcgat gacctcggcc agcccgccgg tggcgatgac tttcatttct cgccccagct
     3541 ccttgcggaa gcgcgccacc atgccctcca ccagtgacac gtacccgaag agcaagccgg
     3601 attgcatggc gtgaggagtg ttgcgcccga tggcggaagg tggtcgctgc aggtcgacgc
     3661 ggggtagctt ggcggtgcgc atgacgagcg cctcggcggc aataccgatg cccggggcga
     3721 tcgccccgcc caggtagtcg ccgtcagcac tgatggcgtc gaaggtggtg gcggtgccaa
     3781 aatccaccac gcaagccggg cctccatata gtcgctgcac ggcgacggca tccgcaatgc
     3841 gatcggcgcc caccgcgcgc gggtcctcgt agcggatgcg caccccggtc ttcaccccgg
     3901 cgtccagcac cagtggatcc agcccaacgt agcgctggca ggcctccacc atgcgcccgg
     3961 tgagcggagg cacgacagaa gccaggcagg caccggtcag ctcattggtc tgatgaccga
     4021 catgggagag caaacctaag agctgcacgc cgtattcgtc gggcatgcgc tcgtgatttg
     4081 tagccagccg ccagcgcggc ccgagcgtct cgcccttata aagacccaat gtaatgttgg
     4141 tgttgccgat atcgatagtc aggagcatgt taattcagat tccgagaaca aagaatagat
     4201 attcgagatt agagaattga gtaataaagg aagcaccact tcctaccctc gaatctcgac
     4261 attgtcgatg aggcgcgtct tgcccacgaa gactgccatg gagagtaagg cgcgggttac
     4321 agatccttgc agttcgacga tcgcatccgg gtgggcacag gagacgtatt gcaagcgagc
     4381 cagcggttca gcattgacca tctctgtaat gcggtcgcgc agttgctggg cgtcgcgctc
     4441 accggaaagg aaggcatccc gcgcggaaaa caaggcgcgt gaaagcacgg ttgctgcctt
     4501 gcgctcggcg gggttaaggt acacattgcg gctggacatt gccaagccat ccggctcgcg
     4561 cacgataggg cagaccacga catcgatggg aaagtttagg tcgcgggtca tctgccggat
     4621 gaccacggcc tgctgggcat ctttctgccc gaagtaggcc ttgtccggct gaacggcggt
     4681 aaagagcttg gcgacaacgg tggtcacacc gcggaagtga ccggggcgca tcgccccttc
     4741 caggggctgg gagacatcat cgacggtgat ccaggtctgg aaaccatttg ggtacatgac
     4801 ctcggttgtg ggtgtccaca ccaaatcgac accgtctgct tccagcagtt gtaagtcgcg
     4861 ctcgaggtcg cgggggtatg atgccaggtc ttcgctgggg ctgaactggg tggggttgac
     4921 aaagatgctc acaatcacgc tggcgcattc gct
//
LOCUS       DBNHOH010000161         2563 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_137151, whole genome shotgun sequence.
ACCESSION   DBNHOH010000161 DBNHOH010000000
VERSION     DBNHOH010000161.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_137151"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1639
                     /locus_tag="AC16V7_02905"
     CDS             <1..1639
                     /locus_tag="AC16V7_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6256098.1"
                     /translation="HAMNDAHTMIETPNRTRKSVRWPSLRAVVMLWIPSILVAGIILL
                     PIIYLVLRAVQAENGLDLILKNTTLLTILRTILLAGAVTLISATLAVPLAWLTTRTDL
                     PLRRLWATLTALPIVIPSYVGAYLIVSTLGPRGMLQEWLEPLGVTRLPAIYGFPGALF
                     ILTILSYPYILLGVRAALQNADPSQEEAARSMGLNPWQTFWRVTLPGLRPAIAAGGLL
                     VALYVLRDFGAVSVMRYNTFTRVIYLQYKSTFDRSAAAILSLVLVVLTLTLLYFEQRT
                     QTKAQYYTNAINAKRTPKVVKLGRWKWVALTFVILVVFAGVIMPTLNLLYWLVRGVLA
                     GEVIPGLWLSTRNSMMASGLAAIVTLLAALPVAIISVRYAGRVSKTIKSITYLAFALP
                     GIVIALALVFFGANYAQPIYQTLPMLIIAYLILFSPEAVGAVHASFLQVHPSMEESGR
                     SLGHSPLAVFRKITLPLIRPGIRASLALVFLTTMKELPATLILSPIGFKTLSTGVWGA
                     VSEAFFAQAAAPALLIILVSSVPMGFMILGEKQPHKT"
     gene            1668..>2563
                     /locus_tag="AC16V7_02910"
     CDS             1668..>2563
                     /locus_tag="AC16V7_02910"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6256099.1"
                     /translation="MTKIAVKTAQLSKSFEPDQSVVHQLDLDVYAGEILALLGPSGCG
                     KTTSLRLIAGFERPDSGSIAINDQTAAGDGLFVPPEKRGVGMVFQDYALFPHLTIHEN
                     VGFGLHNKPKPEANEIVTSMLELVGLQQFASRYPHELSGGQRQRIALARALAPQPFLL
                     LLDEPFSNLDADLRTQMRIEVRQILKSINATAIFVTHDQEEALYLGDRIALMNHGRVE
                     QIGHPEEIFHQPATRFVAEFMGGTDFLPGVVGEEAIETEIGLLPQKVDLPIGTPVEIA
                     MRYDDLSFELNPHSASQVEVRH"
BASE COUNT          525 a          790 c          681 g          567 t
ORIGIN      
        1 gcacgctatg aatgatgctc acacaatgat cgagacccca aaccgcacgc gaaaaagcgt
       61 tcgctggccg agtctgcgtg cggtcgtcat gctgtggatc ccatccatcc ttgtagcggg
      121 catcatcctg ctgccgatca tctatctcgt gctgcgcgca gtccaggctg aaaacgggct
      181 ggacttgatt ctcaagaata ctaccttact gacaatcctg cggacgatcc tgctggcagg
      241 agccgtcacc ctgatctctg ccactctggc ggttccactg gcgtggctga ccacccgcac
      301 cgacctgccg ctgcgccgct tatgggcaac cctgacagcc ctgccgattg tgattccgag
      361 ctatgttgga gcgtatctga tagtttccac cctcgggccg cgcggcatgc tgcaggaatg
      421 gctggaaccg ctgggcgtga cccgcctgcc agcgatctac ggctttccgg gggcactctt
      481 catactcacc atcctcagct acccatatat tctattaggc gtgcgggcag ccctgcaaaa
      541 cgcagatcca tcccaggaag aagcggcccg cagcatgggc ctcaacccct ggcagacctt
      601 ctggcgggtg accttacctg gcctgcgccc ggcgattgcc gcaggcggct tgctggtggc
      661 cctgtatgtg ctgagggatt ttggagccgt ttcggtgatg cgctacaaca cgttcaccag
      721 ggtgatctac ctgcaataca aatccacctt cgaccgctct gctgccgcga tcctctccct
      781 ggtgttggtg gtgctgaccc tgaccctgtt gtactttgaa cagcgcaccc agaccaaagc
      841 acaatactat accaatgcaa tcaatgccaa gcgcactccc aaggtcgtca aattagggcg
      901 ctggaagtgg gtcgcattga cctttgtgat cctggttgta tttgccgggg tgatcatgcc
      961 cacactgaac ttgctctact ggctggtgcg cggtgtgctt gccggagagg tcattccagg
     1021 actgtggctc tccacccgca actcgatgat ggcatcaggt ctggccgcaa tcgtcaccct
     1081 cctggcagcc ctgcccgtgg ccatcatcag tgtacgctat gcgggccggg tgagcaaaac
     1141 gattaagagc attacctatc ttgcatttgc gctgccaggg atcgtcatcg cgttagccct
     1201 ggtgttcttc ggtgcaaact atgcccaacc gatctatcag actttgccga tgttaatcat
     1261 tgcctatctg atcctcttct cccccgaagc agtgggcgca gtgcatgcct cgttcctgca
     1321 ggtccaccca agcatggaag aatctgggcg cagcctgggg cacagccccc tggctgtttt
     1381 tcgcaagatc accctgcctc tgatccgtcc cgggatccgc gccagcctgg cgttggtttt
     1441 tctgaccacg atgaaagaac tgcccgccac cctgatctta tctccgatcg gatttaagac
     1501 gctatccaca ggcgtttggg gggcagtgtc tgaagccttc tttgcccagg ccgccgcccc
     1561 ggcgctgctg attattctgg tttcttcggt ccccatgggt ttcatgatcc tgggtgaaaa
     1621 gcaacctcac aaaacataat tcacctgaag acaaggtcat ctgtcacatg acaaaaattg
     1681 cagtcaaaac agcacaacta tcaaaatcct tcgagcccga tcagagtgtg gtgcaccagc
     1741 ttgatctgga cgtttacgcc ggagaaatcc tggccttact gggacccagc ggatgcggca
     1801 aaaccacctc cctgcgcctg atcgccggct ttgagcggcc agacagcggt tcgatcgcga
     1861 tcaacgatca aaccgctgcc ggggatgggt tgtttgtgcc acctgaaaag cgcggcgtcg
     1921 ggatggtctt tcaggattat gccctgttcc cccacctcac tatccatgaa aatgttggct
     1981 tcggactgca caacaagccc aaacccgaag cgaatgagat cgtaacatcc atgctcgagc
     2041 tggtcggatt gcagcaattt gccagccgct atccacacga gctctctggc ggccaacgcc
     2101 agcggatcgc actggcccgc gccttggccc cacaaccctt tttactgctg ctggatgaac
     2161 ccttcagcaa cctggatgcc gacctgcgca cccaaatgcg gatcgaggtg cgccagatcc
     2221 tgaagagcat taacgccacg gcgatttttg tcactcacga tcaggaagaa gccctgtatc
     2281 tcggagaccg gatcgcgttg atgaaccacg gccgggtgga acagatcggg catccggagg
     2341 aaatctttca ccagcctgcc acgcgcttcg tggccgaatt tatgggcggc accgactttt
     2401 tacccggcgt agtgggtgaa gaggcgatcg agaccgagat cggtctgctg cctcaaaagg
     2461 tggacctgcc gattggcacc ccggtagaaa ttgccatgcg gtacgatgac ctgagttttg
     2521 aactcaatcc ccactcagcc agccaggtag aagtacgcca ctt
//
LOCUS       DBNHOH010000162         1653 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1163470, whole genome shotgun sequence.
ACCESSION   DBNHOH010000162 DBNHOH010000000
VERSION     DBNHOH010000162.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1163470"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="AC16V7_02915"
     CDS             complement(<1..525)
                     /locus_tag="AC16V7_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR6256100.1"
                     /translation="MTHHLSVWTNKHALALYFVLAYAFSWAVEIPIALSVQGIIPTKV
                     PLATHYFASFGPLLAALVVVSVTEGVGGLRRLFQRLLKWRVGAGYALFAIAFPAGLFA
                     VAVVASWIMQGAWPDLGLLNEVDYLPRVGMLPALGLWLLTYGFGEEMGWRGYALPRLQ
                     SGRSAYSAALLLGVL"
     gene            complement(563..991)
                     /locus_tag="AC16V7_02920"
     CDS             complement(563..991)
                     /locus_tag="AC16V7_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PNPOx family protein"
                     /protein_id="HIR6256101.1"
                     /translation="MWYNAMMKWILRSPFHGLISKGVILVTYTGRKSRKAYSVPVNYV
                     QDGNVLYVTSYRVRTWWRNLRGGTALTVRLSGQDKKARGEVIEDDAGVAAGLTAYLQK
                     VPQYAKYYQVKLDPDGTPNAADINQAALDRVIIRIHLGPI"
     gene            complement(1009..>1653)
                     /locus_tag="AC16V7_02925"
     CDS             complement(1009..>1653)
                     /locus_tag="AC16V7_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256102.1"
                     /translation="WTVSYDPDQYDRGPDGYPHPLLWTPDGKYLYYTLRPIVDRRLFN
                     RIFSDGVGLFELNLKNGKQNQILPGSPWPIAMAVAPDSSKVAYISGEFDSPEVIAIRP
                     LGTKSKEGAKQETIIQKPDGFFFPGAMLWSPDGKKLVFVAEEMGSERYSIFLYDPDQA
                     ELKVLNHTDARLLAPQEWSNPDRILVYDYTNDIAWWLDAKNGGLYEGPEVTPMP"
BASE COUNT          378 a          469 c          435 g          371 t
ORIGIN      
        1 gaggacaccc aaaagtaagg cagcggagta ggctgaccgc cccgactgca ggcgcggcag
       61 tgcataaccg cgccagccca tctcctcgcc aaagccgtat gtcaacaacc atagaccaag
      121 cgctgggagc atacccacac ggggtagata atcaacttca ttgagcagcc cgagatcagg
      181 ccaggcgccc tgcatgatcc aggaagcgac gactgccacg gcaaaaagcc ctgccgggaa
      241 ggcgatcgca aacaaggcgt agcctgcgcc aacgcgccat ttgagcaacc gctggaatag
      301 cctgcgcagg ccgcccactc cttctgtgac gctcaccaca accagggcag ccaggagcgg
      361 cccgaaagaa gcgaaatagt gggtggcgag cggcacctta gtagggatga tgccttgtac
      421 ggagagtgcg atggggattt ctacagccca tgagaaagcg tatgccagca cgaagtacaa
      481 ggctaaggcg tgtttattgg tccaaacgga aagatggtgt gtcatcgcag gcttcctctc
      541 ctggtatctt ctgttttgcg ggctaaattg gccctaaatg gatgcggatg atcaccctgt
      601 ccagggctgc ctggttgatg tccgccgcgt taggtgtacc atcgggatca agcttcacct
      661 ggtaatactt ggcatattgg ggcacctttt gaaggtatgc cgtcaaaccc gcagcgacac
      721 ctgcatcgtc ttcgatgact tctccccttg ctttcttatc ctgtccactc aatcgcacgg
      781 taagcgctgt accgccgcgc aggttcctcc accaggtgcg cacccggtag ctggtgacat
      841 ataacacatt cccgtcttgg acatagttta ccggcacact gtacgccttg cggcttttac
      901 gcccggtgta agtgaccagg atgacacctt tgctgatcag gccatggaac ggggatcgca
      961 gtatccattt catcatcgcg ttgtaccaca tggtaagcct ccatttcatc agggcatagg
     1021 cgtgacctct gggccttcgt acaacccgcc attcttggcg tccagccacc aggcaatatc
     1081 gttggtatag tcgtacacca ggattctgtc aggattactc cattcctgag gagcaagcag
     1141 gcgcgcgtcg gtgtggttaa gcaccttgag ctctgcctgg tcagggtcgt acaggaagat
     1201 cgaataccgc tcgctcccca tttcttcggc gacgaatacc aacttttttc catccggcga
     1261 ccagagcatt gcccctggaa agaaaaaccc atccggtttt tgaatgatcg tctcctgttt
     1321 tgccccttcc ttgctctttg ttcctaatgg gcggatggca attacttctg gagaatcaaa
     1381 ttcaccgcta atataggcaa ccttgctgct atcgggcgcg accgccatgg cgatcggcca
     1441 aggcgatcca ggtaagattt ggttttgctt gccgtttttc aaattcaact caaataaccc
     1501 cacaccgtcg gaaaagattc tattgaacag cctgcgatca acaattggtc gaagggtata
     1561 gtacagatat ttgccatctg gcgtccacag cagggggtgg ggataaccat ccggtccgcg
     1621 atcgtattgg tctggatcgt aagacaccgt cca
//
LOCUS       DBNHOH010000163         2108 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_971089, whole genome shotgun sequence.
ACCESSION   DBNHOH010000163 DBNHOH010000000
VERSION     DBNHOH010000163.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_971089"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="AC16V7_02930"
     CDS             complement(<1..706)
                     /locus_tag="AC16V7_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256103.1"
                     /translation="MQLMEKRGSRRLLQLSAGSGVLTSLLLIVGSSLPTGETLSNTLL
                     ALAGFLTPLWMTGVYQIQRHKIGKLGRSGYIIGVVGVLLISARLLAAILETGLGIIEI
                     QRLFATQPGALITGTSIAMFVWGVMALGTASLVAGEIPRIATVLWMVGLMQTVLSISA
                     LSGLFAIAGITWSSYRLWMSAGVERNGISPATPPESSLQATSSKPGGRMLALDALRGL
                     IMILMAIDHASLFIRRS"
     gene            complement(808..1569)
                     /locus_tag="AC16V7_02935"
     CDS             complement(808..1569)
                     /locus_tag="AC16V7_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6256104.1"
                     /translation="MSQDLIQVQHHERIALVSLLKGVTNAIDSKLVEQLSAALSAAAA
                     DPEVHGVVLSSSNEKFFSIGFDIPQLYNLSRDEFTSFYGAFNAVCMQLYSMPKPSAAA
                     VTGHAIAGGCILALCCDRRIIAEGRKLMGLNEIKLGVPVPFVAECIVRQLVSGHHARE
                     ILETGAFYPPEQLLQIGLVDGIVPAGQVLEKAMETVRSYETIPPSAFAAIKQSRIEQV
                     QELVSAKLDERSRIFIECWYSAGARQLLQSAMEKF"
BASE COUNT          493 a          575 c          569 g          471 t
ORIGIN      
        1 gtgatctgcg gatgaagagg ctggcatggt cgatagccat caggatcatg atcaagccgc
       61 gcagcgcatc cagtgccagc atccgccccc caggcttcga ggatgtggct tgaagagacg
      121 actcgggtgg tgttgcaggc gagatgccat tgcgctccac cccggcgctc atccacaggc
      181 gatagctgct ccaggttatc cccgctatgg cgaacagccc gctgagggcg gagatggaca
      241 gcacggtttg catcaagccg accatccaaa gcactgtggc tatgcgcggg atttccccgg
      301 cgacgagcga ggccgtcccc agcgccatca ccccccaaac aaacatcgct atggaggtgc
      361 ccgtgatcag agcgccaggt tgggtggcga acaacctttg tatctcaatt atccccagtc
      421 ccgtctccag gatggcggcg agcagccggg ctgagatcag caggaccccc accaccccga
      481 tgatgtaacc actgcgccct agcttaccga tcttatgcct ttggatctgg tacacgcccg
      541 tcatccatag gggagtcagg aaccctgcca gggcgagcag cgtattcgac aaggtctccc
      601 ctgtgggcag gctgctaccg acgatcaaca acaggctggt gagcacaccc gatcctgcgc
      661 tcagttgtag cagcctgcgg gaccccctct tctccatcaa ctgcatgtac ccaaatcctt
      721 attagattga actgtcatga tgagcgtttt ctgcgcagca atctcttatt cgtcgttatg
      781 tgtggcacac gaagtgcgag gcagctttta gaatttctcc attgcgcttt gtagcagctg
      841 acgcgctccc gctgagtacc agcattcgat aaatatacgg ctgcgctcgt ccagcttggc
      901 ggagaccagc tcttgcacct gctcaatgcg gctttgcttt atggctgcga aagcgctggg
      961 cggaatagtc tcgtatgatc gcacggtctc cattgctttt tccaggactt gcccggcggg
     1021 tacgatcccg tccaccagtc ctatttgcag gagctgctcg ggagggtaga aggcgcccgt
     1081 ttcaagtatt tccctggcat ggtgaccaga gaccagctgc ctgacgatgc actcagccac
     1141 aaaggggacg ggaaccccta gcttgatctc gttaagcccc atcagcttgc gcccctcggc
     1201 gatgatcctg cggtcgcagc acagggcaag tatgcagcct ccagcgatgg cgtgcccggt
     1261 gacggcagcc gcgctaggct tgggcataga atacagctgc atgcaaacgg cgttgaacgc
     1321 gccatagaaa gaggtgaact cgtctctcga tagattgtac agttggggga tatcgaagcc
     1381 gatggagaag aacttctcgt tggagctgga cagaacgacc ccatgtactt ctgggtctgc
     1441 cgccgcagcg ctcagtgccg cagagagctg ttcaaccagc ttgctgtcaa tggcattggt
     1501 gacccccttg agcaggctga cgagggcaat tcgctcgtga tgttgtacct ggataagatc
     1561 ttgggacata tactcctctc ccgggtagcc ggagattggc tacaatccca gattcggtta
     1621 ctgcgcaccc gggtcaatta aaccctaact cgctggtttt gtaaaatact cttctgtttc
     1681 ggaagagcaa tatgaagaca aattggagat cttatggata ttcaccgaaa ttggttaagc
     1741 tcttcggtgt aataacgtcc cgcaggttta tgatcacaca ggttcgttta aagagaacct
     1801 gcgggacgtt caaaaccaat ttatctaaag gtcaatagga agtgtcaaat gcggagcata
     1861 taagcttggt gaatacacaa cacttgtgca atatcattac gaacatccgg cgagagtcgg
     1921 gcgttttata tcaaggcgtg caagcgggac ttacaccgaa tattgtgcat tgcttcacaa
     1981 aattgattct gaggtaaatt tcgtatataa tttgagcata ataacaaata cgacatagat
     2041 gcccatattg ctggcggagg gcaggcaaac atcctagcct gactatcgag acaaaaccat
     2101 agaggacc
//
LOCUS       DBNHOH010000164         2570 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1355603, whole genome shotgun sequence.
ACCESSION   DBNHOH010000164 DBNHOH010000000
VERSION     DBNHOH010000164.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1355603"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="AC16V7_02940"
     CDS             complement(<1..373)
                     /locus_tag="AC16V7_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256105.1"
                     /translation="MHTSRVFGIFVVFLVALFLALVILTGFSQVSATEQTATGQAHLF
                     LAPTIAPNDGPWVVVAYFSNREQVNRVAERIEPWEVNYDQGFMLLEVDKDQYQWLLEL
                     GLQVVIAQELTELLHRPQFALP"
     gene            complement(375..1745)
                     /locus_tag="AC16V7_02945"
     CDS             complement(375..1745)
                     /locus_tag="AC16V7_02945"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HIR6256106.1"
                     /translation="MAQPLGNQVNRDKLMRTVRVRNDQQLGTLGRLLTTVAEERGDIG
                     EIRLVQEGHRANIRDITIFVDDEAQLERVLKAMAGNPGTRILAVRDEVLELHQKGKIA
                     IRSRYQIDSLYTLRRVYTPGVANVCLKIAAEPSLARQYTAISHLVAIVTDGTAILGLG
                     DIGPLAGMPVMEGKAMLMETLVGLSGVPILLNTKDVDTIVKTVASIAPTFAAIQLEDI
                     SAPRCFEIERRLQEQLDIPVMHDDQHGTAVVTTAALFNACRKTGMNLKEAVIGQIGLG
                     AAGNAIGRMLMALTGNSILGADISEDALQRFEQIGGLRSNLSEVMDRANIIIATTGVP
                     GLIKPSQVRQGQIILALSNPNPEIDPDAAIAAGAAFAADGKSVNNVLGFPGILRGSID
                     TYAPRITDEMYLAAAQTIAELTPREELVPNPLDKQVHQAVARAVAQKAIEQGLARAEF
                     VPYLEE"
     gene            complement(1718..>2570)
                     /locus_tag="AC16V7_02950"
     CDS             complement(1718..>2570)
                     /locus_tag="AC16V7_02950"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uridine kinase family protein"
                     /protein_id="HIR6256107.1"
                     /translation="IAQRRDQLRVVLVAGPSSSGKTTFSRRLSVQLLARGISPFPLEM
                     DNYFVDRDQTPLDENGELDFESIGALNHERLSEDLLRLSNGELVQIPDFNFKTGKSTT
                     GEEIELKSNEIIILEGIHGLNPALISGIPSENAYRIYVSALTQLNLDRHNRVSTTDTR
                     LIRRIVRDARERGYTAQQTIQRWDSVRRGEKRYIFPYQENADVMFNSALVYEVSVLKP
                     LVEPLLRQVPYGKPEYIEAKRLLAFVQWFLPLESDLIPDNSIMREFIGESILRDFTLW
                     HNHSETK"
BASE COUNT          594 a          647 c          691 g          638 t
ORIGIN      
        1 ctgggagggc aaattgcggg cggtggagca gttcagtaag ctcctgggcg atcacaactt
       61 gaagtcctag ttctaaaagc cactggtatt ggtctttatc cacctcgagc aacataaagc
      121 cctggtcgta attcacttcc cagggctcaa tccgttctgc gactcgattt acctgttctc
      181 ggttggagaa ataagccacc accacccaag gaccatcgtt tggtgcaatt gttggggcta
      241 gaaagagatg ggcttgtccg gtggcagttt gctccgtggc agagacctga ctgaaaccgg
      301 tcaggataac taaagctaaa aacagagcta caagaaaaac gacgaatatg ccgaaaacac
      361 gggatgtgtg cacgctattc ttcgagatat ggcacaaatt cggcgcgcgc cagcccctgc
      421 tctatcgctt tctgagctac tgctcgggct accgcttgat gcacctgttt atcgagtggg
      481 tttggaacca attcttcgcg gggcgtgagc tctgcaattg tttgggcggc agccaggtac
      541 atttcatccg tgatgcgggg cgcgtaggtg tcgattgagc ccctgagaat gcctgggaag
      601 ccaagtacat tgttgacaga ttttccatcg gcagcgaatg cagccccggc ggcgatagct
      661 gcatctggat ctatttcagg gtttggattg gaaagagcca gtatgatttg cccttgcctt
      721 acctggctag gtttaatcaa accaggtact ccggtggtag caatgatgat atttgctcga
      781 tccatcacct cggataggtt tgagcgcagg ccacctattt gttcaaaccg ttgcagggca
      841 tcctccgaaa tgtcagcgcc gaggattgaa ttgccagtga gcgccatcag catgcgaccg
      901 atggcatttc ctgcagcacc caatccgatc tgaccaatca ctgcttcctt cagattcatg
      961 cctgttttgc ggcaggcatt aaatagggcg gctgtagtga ctaccgcggt gccatgctgg
     1021 tcatcatgca tcactgggat atctagctgc tcttgcaggc gtctttcaat ctcgaaacag
     1081 cgcggggcgg agatgtcctc caattggatg gcggcgaatg tgggagcaat gcttgccacc
     1141 gtttttacga ttgtatccac atcctttgtg ttgagcagga tgggaacacc cgatagcccc
     1201 accagagttt ccatgagcat ggctttgcct tccatcacgg gcatccccgc caaggggccg
     1261 atatctccca gccctaaaat ggcggtacca tctgtgacga tggctacgag atggctgatc
     1321 gccgtgtact gtcgcgccag agatggctca gcggcgattt ttaggcatac attggcaacg
     1381 ccgggtgtat agactcgcct cagtgtgtat aaagagtcaa tctggtatcg gctgcgaatt
     1441 gcaattttgc ctttttggtg tagttccagc acttcatcgc gaacagccag gatgcgcgtg
     1501 ccgggattgc cagccatggc tttcagcaca cgctccagtt gggcttcatc atcaacgaaa
     1561 atcgtgatat cacggatatt cgcccgatgt ccctcttgaa caaggcgtat ctccccaata
     1621 tcgcctcgct cttcagctac tgtcgtaagc aggcgaccga gagtgcccag ttgctggtca
     1681 ttgcgcacgc gcaccgtgcg catcagctta tctcgattta cttggtttcc gagtggttgt
     1741 gccataaagt gaaatctcgc aagatagatt ctccgataaa ctctctcatg atcgagttat
     1801 cggggattaa atcactttcc aggggcagga accattggac aaaggctaac aggcgtttgg
     1861 cttcgatgta ttccggtttt ccgtaaggga cctgccgcaa tagtggctcg acgagaggtt
     1921 ttaatacgga tacctcatac accaaagctg agttgaacat cacgtcagca ttttcctggt
     1981 aagggaagat ataccgcttt tctccacgcc gcacagagtc ccagcgttgt atggtctgtt
     2041 gagcggtata gccgcgctcg cgggcatcgc gcactattcg acgaatcagc cgagtgtcgg
     2101 ttgtggagac acgattgtga cgatctagat tcaattgcgt gagggcggag acatagatgc
     2161 ggtaagcatt ctccgagggg atgccggaaa tcaaagcggg attaagacca tgaatgccct
     2221 ccaagatgat gatttcgttg gatttgagct caatttcttc accagtggtg cttttgccgg
     2281 tcttaaaatt gaaatccggg atttgtacca attcaccatt ggaaaggcgg agcaagtctt
     2341 cagagaggcg ttcgtgattt agcgcgccaa tcgactcaaa atccagctcg ccattctcgt
     2401 caagtggggt ctgatctcgg tccacaaaat aattgtccat ctccaacggg aaaggagaga
     2461 ttccgcgtgc caggagttgc acggagagtc gcctggaaaa agtagttttt cccgatgatg
     2521 aaggcccggc aacgagaacc acgcgcaact ggtcccgtcg ttgggcaata
//
LOCUS       DBNHOH010000165         1186 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1547869, whole genome shotgun sequence.
ACCESSION   DBNHOH010000165 DBNHOH010000000
VERSION     DBNHOH010000165.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1547869"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="AC16V7_02955"
     CDS             <1..552
                     /locus_tag="AC16V7_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HIR6256108.1"
                     /translation="SNFHQAMVRAYLLDNSDDPDFQISVIEEAMSNFHRYFFIMPILA
                     CFELDIHQREERGQGISAEDMNTLMADLFAEGYGDEMKYDRQRVGITWSTFAHLYADY
                     YVYQYATGISGANALSRRILDQTPGAVEDYLGFLKAGGSQYPLDILMSAGVDLTTPQP
                     VEETYAILAGLIDRLERLIENRG"
     gene            542..>1186
                     /locus_tag="AC16V7_02960"
     CDS             542..>1186
                     /locus_tag="AC16V7_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HIR6256109.1"
                     /translation="MEDERNRFRGCLLGLAVGDAVGTTVEFRPRGSFAPLTDMVGGGP
                     FHLLPGQWTDDTSMALCLATSLVDCDGFDARDQMERYNQWARAGYLSSTGSCFDIGNT
                     VNSALIRFRQTGEPFAGSTDPRSAGNGSIMRLAPIPMFFFPDLDEVERYAALSSRTTH
                     GAAECVDACRLFARVIYRALLGRSREEVLFGDSESFDGCKQILAIARGLYRETPK"
BASE COUNT          277 a          288 c          321 g          300 t
ORIGIN      
        1 tcaaatttcc accaggcgat ggttagagca tatttactgg ataactcgga tgatcctgat
       61 ttccagatca gcgtaattga agaggccatg agtaatttcc accgctactt tttcatcatg
      121 cccatattgg cgtgctttga gctggatata caccagcgag aggagcgcgg acagggtata
      181 tcggcagagg atatgaatac cctcatggct gacctatttg ctgaaggcta tggagacgag
      241 atgaaatacg accgccagcg ggtaggcatc acctggtcta ctttcgcaca tttatacgcc
      301 gattactatg tataccaata cgcaaccggc atctcaggcg caaatgcact ctcgcgcagg
      361 atcctcgacc aaacgcctgg tgccgttgag gattatttag gtttcctaaa ggctggcggg
      421 tcccaatacc ccttggacat tcttatgagt gccggtgtcg atctcaccac gcctcagccg
      481 gttgaagaaa cgtatgcaat tcttgcagga ttgatcgacc gcctggagcg attgattgaa
      541 aatagaggat gaacggaata gatttcgagg ctgccttctc ggtcttgctg tcggggatgc
      601 ggttggcacc acggttgagt ttagaccgcg ggggagcttt gcccctttga cggacatggt
      661 tggtggaggc ccttttcacc ttttgccagg tcaatggact gatgacacat ctatggcgct
      721 ttgcctggca accagccttg tggattgcga tggatttgat gcccgtgatc agatggaacg
      781 ttacaaccaa tgggctcgtg caggttactt gagcagcact ggctcgtgct ttgatatcgg
      841 aaacacagtt aactctgctc ttattaggtt tcgccaaaca ggagaaccat ttgctggatc
      901 aaccgatccg cgctcggctg gcaatggcag catcatgcgc ttggcaccca tccccatgtt
      961 tttcttcccg gacctggatg aagtagagcg atatgcagcg ctgagttccc gcacgaccca
     1021 cggcgctgca gagtgtgtag acgcatgtcg cctattcgca agggttatct atcgtgccct
     1081 attgggcaga tcgagagaag aagtgctgtt tggagacagt gaatcgtttg atggatgtaa
     1141 acaaatcctg gcaattgccc gtggactata ccgtgagacg ccgaaa
//
LOCUS       DBNHOH010000166         6087 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1420211, whole genome shotgun sequence.
ACCESSION   DBNHOH010000166 DBNHOH010000000
VERSION     DBNHOH010000166.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6087)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6087
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1420211"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1986)
                     /gene="ppk1"
                     /locus_tag="AC16V7_02965"
     CDS             complement(<1..1986)
                     /gene="ppk1"
                     /locus_tag="AC16V7_02965"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HIR6256110.1"
                     /translation="MVATAEQVTDIKDLPVDLNDPKLYINRELSLLEFQRRVLEEARD
                     TSNPLLERVKFLAILGSNMDEFYMVRVSGLKKQLDAGIIDLPPDGLTPAEQLAAIRKM
                     ALQLMTQSRECLTKDIMPALNKAGIHIMDYKDLDERQKSNVKAYFDEVVFPVLTPLAF
                     DPGHPFPHISNLSLNLAVLICDETQRKHFARIKVPNTLPRLLPIKRSSGGVRKDGTVP
                     YHHYFVWLEQVIAANLDALFPGMEILEAHPFRVTRDADMEIQELEADDLLESMELNVR
                     QRRFGSVVRVTLNDDMPAHVREILIKNLEVERNDIYTLFGPLGLSDLMQLYHIDRHDL
                     KDAPFVPATPLPLKSEQYDGNLFAAIRKEDILLHHPYESFLPVVDFLKMAARDPDVLA
                     IKQTLYRVGRNPPVVQSLLEAVEKGKQVAVLLELKARFDEESNIGWARKLESAGVHVI
                     YGLLGLKTHSKVALVVRKEGEGIRRYIHLGTGNYNAVTAQQYEDMGMFTCDDEIGADA
                     TDLFNYLTGYSNKKEYRKLLVAPINLRERMQALIQREIEHQKNGEKGHLIFKMNSLVD
                     KPIIQELYQASQAGVKVDLLVRGICCLRPGLNGISENIQVTSILGRFLEHSRIFYFRN
                     AGKEEVYLGSADLMPRNINRRVEVLFPVQERHMIRYLR"
     gene            complement(2084..3328)
                     /locus_tag="AC16V7_02970"
     CDS             complement(2084..3328)
                     /locus_tag="AC16V7_02970"
                     /inference="COORDINATES: protein motif:HMM:NF024919.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HIR6256111.1"
                     /translation="MRKPTLTTMFGVLIALAILGILLYQLPPIKQRLSWRIDFAMAYL
                     RGVVRPVGQVPTALPQPVVRVTSNPTPTEIVVQTSIPATATPQLPTPSPTPLPSSVSL
                     SAPAWERQDINNCGPASLAMYLRYYGWEGDQFDIAELLKPQREDRNVNVEELVYYVRT
                     RAGWLNAIYRVGGDITLLKKFLAAGIPVMIEETFYFADPFWPNDDLWAAHYNLVTGYD
                     DGDQLFTGQDSFYGADQKIAYSKLEEYWKAFNYVYILVYPPEKEETVRAILGPDWDEK
                     VNRQRTLEAAQAAANADPGSSFAWFNLGSNLVYFERYNDAVTAFDRARAIGLPQRMLR
                     YQFTPFFAYFHTRSMEELFTLTEYALKITPNAEEAMLWHGWANYRAGSTSAAISDFQA
                     ALGENPNYQDAIYALDFVRANP"
     gene            3532..4002
                     /locus_tag="AC16V7_02975"
     CDS             3532..4002
                     /locus_tag="AC16V7_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006003094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6256112.1"
                     /translation="MIELNIPGRGTLQLEHLVCDVNGTLAVDGQLIEGISRLIRNLRD
                     RLTVHLITADTHGRQSLIDQQLGLQAARLKPGDEARQKAEFVRQLGAEGVIALGQGAN
                     DANMLKEAALGICVLSKEGTAVEALLAADLLAPDIFFALELLEKPLRIVASLRR"
     gene            4007..5188
                     /locus_tag="AC16V7_02980"
     CDS             4007..5188
                     /locus_tag="AC16V7_02980"
                     /inference="COORDINATES: protein motif:HMM:NF016013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HIR6256113.1"
                     /translation="MSLPKTEQFPDDPESLPPARRRRVRRLLAPLNADERADYLDEFA
                     HRASPTFDFFLFSLVSGAVMGIGLLLDAPALLVLGAVLAPLMAPTIGLSLGTVTGSGR
                     YFLRSLLGVLIGGLLASLGGAAAGLATQYWVSPELTQAYFHAQLSWSNFLVLAIGACY
                     TAAAMLHDKHNPALPSVALAYELYIPLAIAGFGLTSGAPHLWPDGLVIFAIHLAWSAL
                     LGTITLAILGFRPLTIFGYTIGGVVTLLVITLLIGLSGAGAILGGQMALPTPVPTATF
                     TITPTLTRTSTPVPPTATLTPTLTFTPTSTATQTVTPTPTPIYAIIQTTDQKGAVLRA
                     QPGGEVLRSYFDGTLMQVLPDQVTIDGVEWVHVIAPDGLEGWMVQRLLATATPAPEWG
                     G"
     gene            5314..>6087
                     /locus_tag="AC16V7_02985"
     CDS             5314..>6087
                     /locus_tag="AC16V7_02985"
                     /inference="COORDINATES: protein motif:HMM:NF048802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7544 domain-containing protein"
                     /protein_id="HIR6256114.1"
                     /translation="MDIGYVLSRAWQIIWKNKVLWVFGILAGCANGGGGGGGGGNVSN
                     TFQRDFPGGQPFERMFENIPDYQIWGLIAIVILVILVLVVLAIFLGTIGRIGLIRGTQ
                     QAEGGATSLVFGELFSGSMPYFWRVFGLSLLVGLVILVAVGLFVLLAFTGTVATLGLG
                     LVCLSPLIYILVPVMWFINVIVEQASIAIVLENLGIMDGLKRGWEVVRANLGTMVIMA
                     LILLLGVGGIGGLIIALPILIIVVPAIIGGVVGTDQAIGG"
BASE COUNT         1316 a         1677 c         1708 g         1386 t
ORIGIN      
        1 gcgcaggtag cggatcatat ggcgttcctg cactggaaag agcacctcca cgcgccggtt
       61 aatattacgc ggcatcaggt ccgcgctccc tagatagacc tcttccttcc ctgcattgcg
      121 gaagtagaag atgcggctat gctccaaaaa gcgtcccaaa atgctggtga cctggatatt
      181 ctcactgatg ccgttcaaac ccggtcgcaa acagcatatg ccgcgcacca gcagatccac
      241 cttcacacca gcctgtgagg cctggtaaag ctcttgaata atgggcttat ctaccaagga
      301 gttcatctta aagatcagat gacccttctc gccgttcttc tgatgttcaa tctcccgctg
      361 gatgagcgcc tgcatccgct cgcgcaggtt gattggcgcc accaatagct tgcgatattc
      421 tttcttgttc gagtaacctg tcaggtagtt aaacaggtcg gtggcatctg cgccgatctc
      481 gtcatcacag gtgaacatac ccatgtcctc gtactgctgg gcagtgacgg cgttgtagtt
      541 gcccgtcccc aggtggatgt aacgacggat gccctccccc tccttgcgca ccaccagggc
      601 gaccttggaa tgggtcttca agcccagcaa gccgtagatc acatgcactc cggccgactc
      661 cagctttctc gcccaaccta tattgctctc ctcgtcgaag cgcgccttta gctccagcaa
      721 cactgccacc tgtttccctt tttccaccgc ttccagcaag gattgcacta ctggcggatt
      781 gcgaccaaca cggtagagcg tttgcttgat cgccaggacg tccgggtcgc gggcggccat
      841 ctttaggaaa tccaccactg gcagaaaaga ctcgtaggga tggtgtaata agatatcttc
      901 cttacggatc gcggcaaaca ggttgccatc atactgctca gatttcaacg gcagcggagt
      961 agcaggaaca aagggcgcat ccttgagatc atggcgatcg atatggtaga gctgcatcag
     1021 gtcgctcaaa cccagcgggc cgaagagggt atatatatcg ttacgctcta cctccaggtt
     1081 tttgatcaaa atctcgcgga cgtgggctgg catatcatcg ttcagcgtga cgcgtactac
     1141 agacccaaag cgccgctggc ggacatttag ctccatggat tcgagcaagt cgtcggcttc
     1201 cagctcttgg atctccatat cagcatcccg ggtcacgcga aacgggtgtg cttctaagat
     1261 ctccatgccg gggaaaagtg cgtccaggtt ggcggcaatg acttgctcca gccagacaaa
     1321 ataatggtgg taaggcacag tcccgtcttt gcgcacgccg ccggatgagc gcttgatggg
     1381 caataagcgc ggcagggtat tgggcacttt gatgcgggcg aagtgttttc tttgggtttc
     1441 gtcgcagatc aagaccgcca gattgaggct gagattagag atatgcggaa acggatgtcc
     1501 aggatcaaag gccagcggag taagcaccgg gaagaccact tcatcaaaat aggccttgac
     1561 gttcgacttc tgtcgctcat cgaggtcctt ataatccata atgtggatgc cagctttatt
     1621 cagggcgggc atgatgtctt tggtcaagca ctcgcgcgat tgggtcatta attgcagggc
     1681 cattttacgg atagcagcga gctgctcggc tggggtaagc ccatcgggag gcaggtcaat
     1741 gatgcccgca tcaagctgct ttttaaggcc ggatacgcgc accatgtaga actcatccat
     1801 gttcgatccc aggattgcca ggaacttaac ccgttcaagc aaagggttgc tggtatcgcg
     1861 tgcttcctcc agcacccgcc gctggaactc cagcaagctc agctcccggt tgatatacag
     1921 cttaggatca ttcaggtcaa ccggtaaatc ctttatatcg gtaacttgct cagcagtcgc
     1981 gaccatattc ctcttcctta ataattggat gtactataac aaattttgac agcgctggca
     2041 agcggaagaa caacagcgga agaagagaga ggctggatga tctctatggg tttgcgcgta
     2101 cgaaatccaa ggcgtagatt gcatcctgat aattggggtt ttcacccaag gcggcttgga
     2161 aatcagagat ggcagctgac gtactcccag cgcgataatt cgcccatcca tgccaaagca
     2221 ttgcttcctc agcgttgggt gtgatcttga gcgcgtattc tgtcagggta aacaactctt
     2281 ccatgctccg cgtgtggaaa tatgcaaaga agggagtgaa ctggtagcgc aacatgcgct
     2341 ggggtaagcc gatggcgcgc gcccggtcaa aggccgtcac agcatcattg taacgctcga
     2401 agtacaccag gttgctaccc aaattgaacc aagcaaagga gctcccgggg tcggcgtttg
     2461 cggcagcttg tgcagcctcc agcgttctct ggcggttaac cttctcgtcc caatccggcc
     2521 cgaggatggc tctgaccgtc tcctccttct ctgggggata gaccaggata taaacgtaat
     2581 tgaaggcctt ccaatactct tccagtttcg agtaggcgat tttctggtct gcgccgtaga
     2641 agctgtcttg cccggtgaaa agctggtcgc cgtcatcata gccggtcacc aggttatagt
     2701 gcgccgccca cagatcatcg ttaggccaga agggatcagc gaagtaaaag gtctcttcta
     2761 tcatgaccgg gatgcccgcc gccaggaact tcttgagcag ggttatgtcg cctcccacgc
     2821 ggtagatggc attcagccag ccggcgcgcg tgcgcacata atacaccagc tcttccacgt
     2881 tgacatttct atcctctcgc tgcggcttta atagttcagc gatgtcaaat tggtcgcctt
     2941 cccagccgta atagcgcagg tacattgcca ggctggcagg accgcaattg ttaatatcct
     3001 gccgttccca agctggggcg ctgagtgaga ccgaagaggg aagaggcgta ggtgatggag
     3061 tcggtaactg gggagtggcg gtagccggga tcgaagtctg tacgacgatt tcggtggggg
     3121 ttgggttgga agtcacccgc accacaggct gcggcaaggc agtgggcacc tgacccaccg
     3181 gtcgcaccac gccgcgcaga taagccatgg caaaatctat gcgccacgac agacgttgct
     3241 ttataggcgg gagctggtag agcaagatac ctaatattgc cagggcaatc aggaccccaa
     3301 acatggtggt cagtgttggc ttacgcatga ggcggattct aatttgagaa tgtagccaga
     3361 agattagaga ttagtgaagg aatgggataa tatatagacc aacaaacact atagggtaag
     3421 cagtgctcta aaatctcaac tatcagatcg ctcgcagggt tgcatgccgc cttacgggaa
     3481 acgggaaacc gtggtctcga ccatgcctct ctattcctgt ataataacct tgtgatcgag
     3541 ctcaatattc cgggacgggg aacgctccag ctggaacacc tggtttgtga tgtcaatggc
     3601 acgctggcgg tggatggtca gctcatcgag ggcatctccc ggttaatccg caacttgcgc
     3661 gaccgcttga ctgtgcacct gatcaccgca gatacacacg ggcgccagtc actcattgac
     3721 cagcagctag gcttgcaagc tgcccgcctg aaacccggtg acgaagcgcg gcagaaagcc
     3781 gagtttgtgc gccaattggg tgccgaaggc gtgatcgcac ttgggcaggg ggccaacgac
     3841 gccaacatgc tgaaggaagc cgctttgggg atctgcgtgc tgtctaaaga gggcacagct
     3901 gtggaggcct tgctggcagc cgacctgctc gccccagata tcttctttgc tttggagctt
     3961 ctcgaaaaac ctttacgcat cgtagccagc cttcgccgtt aaacctatga gcttacccaa
     4021 gacagaacag tttcctgacg acccagaatc cctgccacct gcccgccgcc gcagggtgcg
     4081 ccgcttgttg gctcctctaa atgccgatga acgcgctgac tatctcgatg agtttgccca
     4141 tcgcgcttcg ccgacctttg atttcttcct gttctccctg gtatccggcg cagtgatggg
     4201 gatcggcttg ctgctagatg ccccggcact tttggtgtta ggggcggtgc tggccccctt
     4261 gatggcgccc accataggcc tttcattggg cacggtaacc ggctcggggc gctatttcct
     4321 acgcagcttg ctgggcgtgc tcatcggtgg cctgctagcc agcctggggg gcgcggccgc
     4381 cggactggct acccaatatt gggtttcacc agaattaacc caggcgtatt tccacgccca
     4441 attatcctgg tcaaacttcc tggtgctggc aatcggagca tgctacaccg cggcggctat
     4501 gctgcacgac aaacataatc ccgccttgcc cagcgtggca ctggcttacg agctctacat
     4561 tcctttagcc attgctggct tcggactgac cagcggcgcg ccgcacctct ggcccgatgg
     4621 tctggtgatc tttgccatac acctggcctg gagcgcccta ctaggaacta taacactggc
     4681 aatcttgggt ttccgcccgc tgacaatttt cggttacacg attggtggag tggtcacctt
     4741 gttggtcatc acgctgctga ttggcctgag cggcgcgggg gcgatcctgg gcgggcagat
     4801 ggccctgccc acacccgtac ccaccgcgac gttcaccatc acccccacgc tcaccagaac
     4861 ttccacgcct gtacctccaa ccgccacgct cactcccacg ctcacattca ccccaactag
     4921 taccgccacc caaacggtca cccccacacc caccccgatc tacgccatta tccagacgac
     4981 ggatcagaaa ggcgccgtgc tgcgcgccca gccaggcggc gaggtactgc gcagttattt
     5041 cgacgggacg ctcatgcagg ttctcccaga ccaagtgacc attgacgggg tggagtgggt
     5101 gcacgtcatt gctccagatg gtctggaagg ttggatggtg cagcgcctgc tggcgactgc
     5161 cacgccagct cccgagtggg gaggttgaaa gttatacatc acattatgag ttatcccggc
     5221 atgaaaccta tctccatgta aatcgtatac aataatactg atggattgat tgctatcaaa
     5281 gtcagaggtt acacagttca aggagaatgc aaaatggata tcggttacgt actctcaaga
     5341 gcctggcaaa ttatctggaa gaacaaagtg ctgtgggtct tcggcatcct ggctggctgc
     5401 gcaaatggcg gtggaggggg agggggaggc ggcaacgtta gcaacacctt ccaaagggat
     5461 ttccctggag gacaaccttt tgaaaggatg ttcgaaaaca tccctgatta ccagatttgg
     5521 gggctgattg ccatcgtgat cctggtgatc ctggtgctgg tggtgctggc aattttcctg
     5581 ggcacgatcg ggcgcatcgg cctgatccgc ggcacccagc aggcggaggg tggcgcaacc
     5641 agcctggtct tcggagagct gttcagtggc agcatgccct acttctggcg ggtctttggc
     5701 ctgagcctgt tagtgggcct ggtgatcctg gtagcggtag ggttgtttgt gctgcttgct
     5761 ttcacaggga ccgtggccac attgggactt ggcctagtgt gcctcagtcc cttgatctac
     5821 atccttgtgc cggtaatgtg gtttatcaac gtaatcgttg agcaagccag catcgccatt
     5881 gtgctcgaga atctgggcat catggacggg ctaaaacgag gctgggaggt ggtgcgcgcc
     5941 aacctgggga cgatggttat catggcgctc atcctactgc tgggtgtggg tggcataggc
     6001 gggttgatca tcgccctgcc gatcctgatc attgtagtac cggcgatcat tggcggggtt
     6061 gtgggcacgg atcaggctat cggaggc
//
LOCUS       DBNHOH010000167         1704 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1612113, whole genome shotgun sequence.
ACCESSION   DBNHOH010000167 DBNHOH010000000
VERSION     DBNHOH010000167.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1612113"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="AC16V7_02990"
     CDS             <1..241
                     /locus_tag="AC16V7_02990"
                     /inference="COORDINATES: protein motif:HMM:NF016993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HIR6256115.1"
                     /translation="QLFQPIAGESELIQAGYVIASENLKDKWQDKVYPTLIESSLVIP
                     ATPRLQLNRQTHTHHLEVLLSEMQSVARLEADANW"
     gene            251..727
                     /gene="paaD"
                     /locus_tag="AC16V7_02995"
     CDS             251..727
                     /gene="paaD"
                     /locus_tag="AC16V7_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008880601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HIR6256116.1"
                     /translation="MTPEEAWRILDEIKDPEIPVVSIVEMGMLRHVEIEDQTVTVTIA
                     PTFVGCPAIQVIQEHVTRSLEEAGAETVNVRFTYSPPWSSDAITPEARKKLKDFGLSP
                     PQMHQGLIEIALLDPAACPYCDSTNTSMRNSFGPTLCRSIHYCNNCQQPFEAFKPL"
     gene            752..1054
                     /locus_tag="AC16V7_03000"
     CDS             752..1054
                     /locus_tag="AC16V7_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016427281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HIR6256117.1"
                     /translation="MSKLIALFKQPADPTAFDQAYFNTHLPLIAKVPGLNKTVVTRFT
                     RTLQGEGFYLMAEMVFDDREALKAGMKSPEMAAAGANLNTFAAGLVTLMFAEEESR"
     gene            1060..1602
                     /locus_tag="AC16V7_03005"
     CDS             1060..1602
                     /locus_tag="AC16V7_03005"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6256118.1"
                     /translation="MSTYTVIDSQTLFEHPGVKIVVDTLDHLGKQRRYFYLASPVNAV
                     ATVGLTDDSQLLLTRQYRHPVGQVIYDLPAGRLNAGESPLEGARREFEEETGYYPNHI
                     EQLGFYNQFPGTLRAATTLFFARDLVPTHQHLDEGEELDIVIKPVPEVLEMILGNEII
                     DGSLQLGVLLALQKGLIKSE"
BASE COUNT          455 a          457 c          403 g          389 t
ORIGIN      
        1 gcagctattc cagcccatcg ccggggaaag cgaattaatc caagccgggt atgtcatcgc
       61 atcagaaaat ctaaaagaca agtggcagga taaggtctat ccaacactga tagaatcttc
      121 actggtcata cctgctaccc cgcgattaca attgaatcgc caaacgcaca cacaccacct
      181 ggaggtgcta ttgagcgaga tgcagtcggt ggcccgcttg gaagccgacg caaattggtg
      241 agagaccaaa atgacccccg aagaagcctg gcgcatatta gatgaaatca aagatcccga
      301 gattcccgtg gtctctattg ttgagatggg gatgctgcgc catgtagaga ttgaagacca
      361 gacagttacc gtcactattg cgcccacctt cgtaggttgc ccggctattc aggtcataca
      421 ggagcatgta acccgctcgc tagaagaggc gggtgcagaa accgtcaatg ttcgctttac
      481 ctattcacca ccttggagca gtgatgcaat cacacccgaa gctcgaaaaa aattgaagga
      541 tttcggccta tcccctccac aaatgcatca gggattgatc gagatagcac tgctggaccc
      601 tgcagcttgc ccatattgcg attcaaccaa tacatccatg cgcaatagtt tcggccctac
      661 cctttgccgg tcaatccact attgcaacaa ctgccagcag ccgtttgaag cctttaaacc
      721 tctctgaaag cttcgacagg agattacaag aatgtccaaa ttgattgccc tatttaaaca
      781 acccgctgat cccacagcat ttgaccaggc ttacttcaat acccatctgc ccctgatagc
      841 aaaagtaccc ggtttaaaca agaccgttgt cactcgcttc acccgaactc tacaaggcga
      901 aggcttttat ttaatggccg aaatggtctt cgacgaccgt gaggcgctaa aagcaggcat
      961 gaaaagtcca gagatggctg cagcaggagc aaacctgaat acatttgccg cagggctggt
     1021 caccttgatg ttcgcagaag aagaaagccg ctagcaccca tgtccaccta cactgtgatc
     1081 gacagccaga ccttattcga acatccgggt gtcaaaattg ttgtcgatac actggatcac
     1141 cttgggaaac agcgccgcta cttttacctt gccagtccag taaacgctgt tgcaacggta
     1201 gggttaaccg atgatagtca gcttctccta acgcgccagt accgccatcc agttggacag
     1261 gtaatttacg acctcccggc tggacgactc aacgcaggtg agtcgccact ggagggcgcc
     1321 cggcgcgagt ttgaagagga aactggctat tatcccaacc atattgagca gcttggcttt
     1381 tacaaccaat ttcctggcac attgcgagcc gcgacgacct tgttcttcgc ccgcgacctc
     1441 gtccctaccc accaacacct ggatgaaggt gaggagctcg acattgtgat caagcctgtg
     1501 cccgaagtat tggagatgat cttgggtaac gagatcatcg atggctctct ccaactcggg
     1561 gtattacttg cccttcaaaa agggctgatt aaatctgaat gatccgtttc cagggcagtt
     1621 agccttcttg aagtagggct gcttttcaaa ggagcatgat ggaatatcgt tacctgggtc
     1681 gcacgggtat taaagtatct gaac
//
LOCUS       DBNHOH010000168         1943 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1612125, whole genome shotgun sequence.
ACCESSION   DBNHOH010000168 DBNHOH010000000
VERSION     DBNHOH010000168.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1612125"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            349..>1943
                     /locus_tag="AC16V7_03010"
     CDS             349..>1943
                     /locus_tag="AC16V7_03010"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015500609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR6256119.1"
                     /translation="MSVFTEEDTLTSMAPTGEPLWELRNISKAFPGVQALDDVSFEVY
                     PGEVHALLGENGSGKSTLAKCLAGVHQAESGQMLFEGREVIFRNPMEARQKGIATIYQ
                     EFSLVPTLTVAENIFLGRYPRNAAGTIDWNTARKATSEVLESLSLTIDPDAIVKGLSV
                     AEQQLVEIAKAISLKSNLLIMDEPTAALGLVETQHLMELIHRLTAQNKAIIYISHRLD
                     EVFQIADRVTILKDGRRVKTARLADFQMGEVVRLMIGFDIQQHYPKAHNTQAIPCLEV
                     EDLSTENGVHNVNFTVNVGEVFGLGGMLGSGRTEIARAIFGMDKLAYGRLRLNGREVR
                     FRSPSEAVAAGIGLIPENRKTDGCFFNFEAPKNITISRLREVLSGPFLSLSKEYQVGK
                     SYISKMNIHPTALEKSVKFLSGGNQQKVIVARWLFSQARLLIMDEPTQGIDIGAKLEV
                     YNVINELTAAGISILLISSDFPELLAMSDRVAIVRDGHILHITEAHRMSEYQLIGMAS
                     GIGLSQEIEMWMDLRKIAHPYLQS"
BASE COUNT          477 a          515 c          552 g          399 t
ORIGIN      
        1 gcggatggac cgagaccccc accgtcaaca ccgataagga caacgccggc agcttcctgt
       61 gcaagcccga aaacctctac cccaaaccat ctaaggagtt tgcctgcccg taagctagtc
      121 acgaaaatcg ccttgcgaag gtttgacgga tactgagagc gaccttcgca aggctactaa
      181 tttcaggaga atggccttgc gaaggtcgta ttgcactgtc ttcaagccaa cggacaagct
      241 tcgcaaggct tcttctctgg ataggattgg atgttctgat tgccgaggtt tattgtaaaa
      301 tcataggtaa ggcaagaatg aattacacag gtgtcatagg agatttgtat gagcgtcttc
      361 actgaggagg atacccttac aagcatggct ccaaccggag agccgctctg ggagctacgc
      421 aacatctcca aagcattccc gggggtccaa gccctggatg acgtctcgtt tgaagtttac
      481 cccggagaag tgcatgccct gctgggggag aacggaagcg gcaagtcaac tctggcgaag
      541 tgcctagcag gcgtacacca agcggagagc ggacaaatgc ttttcgaggg ccgcgaagtt
      601 atttttcgga atccgatgga agcgcgacag aaggggatcg cgacgatcta ccaggagttt
      661 tcgctggtcc ccacgctgac cgtagcagag aatattttcc tgggacgtta ccctcgaaat
      721 gctgcaggca ccatcgattg gaacacagcc cgcaaggcga cgagcgaggt gctggagagc
      781 ctatccctga ccatcgatcc ggatgctatc gtgaagggac tctccgtcgc cgaacagcag
      841 ttagtcgaaa tcgccaaagc gatctcgctg aaatccaact tgctaatcat ggatgagccc
      901 actgccgcgc tgggcctggt ggaaacccag cacctgatgg agcttatcca ccgcctgacc
      961 gcccagaata aagccatcat ctatatctcc catcgcctgg acgaggtttt ccagatcgct
     1021 gatagggtta ctattttgaa ggatgggcgc cgggtgaaaa cagcgcgatt agccgatttt
     1081 cagatggggg aggtggtgcg cttgatgatc ggcttcgaca tccagcagca ctaccccaag
     1141 gcgcacaaca cacaggcgat accctgcctg gaagtggaag acctgagcac ggagaacggt
     1201 gtgcataacg tcaatttcac tgtaaacgtg ggtgaggtct ttggattggg tgggatgctc
     1261 ggctccggtc gcacggagat agcacgtgct atcttcggta tggataagct ggcgtacgga
     1321 agactccgtt tgaatgggcg agaagtgcgc ttccgttcac cgagcgaggc tgtagcagcc
     1381 gggatcgggt tgatccctga gaaccgcaag acggatggct gtttcttcaa ttttgaagcc
     1441 ccgaagaaca tcacgatctc ccggttgagg gaagtgctga gcggcccctt cctcagcctt
     1501 tccaaagagt accaggtggg gaagagctac atcagcaaga tgaacatcca cccaactgcc
     1561 ctggagaaat ccgttaaatt cctctcagga ggcaaccagc aaaaggtgat cgtggcgcgc
     1621 tggctgttct cgcaggcacg cctgctgatc atggatgaac ccacccaagg gatcgatatc
     1681 ggcgccaaac tggaggtgta taacgtcatc aacgagctga cagcggcggg tatcagcatc
     1741 ctgcttatca gctccgattt ccccgaactg ctggcgatga gcgaccgggt agccatcgtg
     1801 cgcgacgggc atatcttgca catcacagaa gcgcaccgta tgtctgaata ccagttgatc
     1861 ggcatggcat ccggaatagg cttgagccag gagatcgaga tgtggatgga cctgcgcaag
     1921 attgcccacc cctacctgca atc
//
LOCUS       DBNHOH010000169         3288 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1035673, whole genome shotgun sequence.
ACCESSION   DBNHOH010000169 DBNHOH010000000
VERSION     DBNHOH010000169.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1035673"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="AC16V7_03015"
     CDS             complement(<1..632)
                     /locus_tag="AC16V7_03015"
                     /inference="COORDINATES: protein motif:HMM:NF036766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR6256120.1"
                     /translation="MTENKQGAIHTCQIALMPSGRKGSVSQGINLLKAAQELGVELES
                     ICGGRQTCGKCQVVVEEGHFPKHALTSRADHLSPPTSREIEYSQEHRLQGRRLACAAE
                     VLGDVLISVPEESQARKQVIAKAASERSIEIDPAVRQVYVEMHPSDLSDSRGDWERLQ
                     EALEAQWGLKGLDIDSSALATLQIALREGDFSVTVNVWQEREVLRIQPGY"
     gene            complement(658..2193)
                     /locus_tag="AC16V7_03020"
     CDS             complement(658..2193)
                     /locus_tag="AC16V7_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR6256121.1"
                     /translation="MIHSFAALLSQEQVEQVHEASLEILERVGMLVRNERAREIFAAH
                     GAQLEEQTQIVRIPRQVVERYRVLFPPKFTFRARDPRYDRTLPDDSPLILTGSSAPNI
                     IDPVTGYERRSRSDDLARISHLINELDGYDVFSISTLADDAPKGQFSLSRYYPSIKNT
                     VKPVRANTPPAEADMMLSLAYLVAGGEDAFRERPFITHHYCPVVSPLTMDFDSTQLLI
                     YFSEKNLPSYFSVVPNAGLTSPLTMLATLAQCNAEFLGAAVLTQMIQPGKAVIYSTLP
                     TVADMRTGAYSPGAIESGILHMGCAQMARFYNIPSGGYIGLTNSKINDAQSGYETGMS
                     VVAGYLGGVDIFNMGGLLDALMAFDFAKAVIDNEIALMMKQIKRGFDFSRENFALDQI
                     AEIGPAGMFAGTEHTLERMRTTMLLAEVADRDPRAQWIERGGLDSHARAMKRVKDILT
                     RENPAAFSSELDEKIRAAFPGMVAGDSLPPEGWKRVTVVDEEGDAGMRRRRRLHRAVA
                     TEA"
     gene            complement(2190..2969)
                     /locus_tag="AC16V7_03025"
     CDS             complement(2190..2969)
                     /locus_tag="AC16V7_03025"
                     /inference="COORDINATES: protein motif:HMM:NF019322.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR6256122.1"
                     /translation="MIAPYPTHSLAYSIPLYIQIAEGLISQVESGELAPGDQLPSERE
                     LSEKLGVNRMTLRRALRVLESQGLVVRRHGVGTFIAEPKIDRRMDSVFRFTRGMQNRG
                     YTPGTRLISLEQTFLDAATARELNLPISLPAYRIRRVRTVNQEAVLLESYTLPAPRFP
                     GLEAHDLESRSVYEVMESEYGVTIARARQSFEPVVASAFEAELLAVRVGAPLMLEKRI
                     SYDVDDRPVEYGKDRYRGDRFRFVTEATPFDLRLLSMEDKP"
BASE COUNT          752 a          950 c          901 g          685 t
ORIGIN      
        1 tgatagccag gctggatgcg cagcacttcg cgctcctgcc atacgttcac tgtcactgaa
       61 aaatcgcctt cacgcaaggc gatttgaaga gtcgccaggg cgctactatc tatgtccagc
      121 cccttcagcc cccattgtgc ttccagcgcc tcctgcaacc gctcccagtc accgcgcgaa
      181 tcgctcaggt ctgatgggtg catttctacg tacacttgac gcacagcagg atcaatctcg
      241 atgctgcgct cagaagcagc cttggcaatc acctgcttgc gcgcctggct ctcctcggga
      301 accgagatca gcacgtcacc cagcacttcg gcagcgcatg ccaggcgacg cccttgcagg
      361 cggtgctcct ggctatattc gatctcgcgg gaggtaggag gggaaaggtg atcggcgcgc
      421 gaggtgagag catgctttgg aaaatgccct tcttcgacga ccacctggca cttgccacag
      481 gtttgccgcc cgccacagat agattccagc tccacgccaa gctcctgggc tgccttgagc
      541 aaattgatgc cctgagacac ggaacccttg cgcccagaag gcatcaatgc aatctggcag
      601 gtatggattg cgccctgttt attttccgtc acgagttttg cctgctatgt tgactcatta
      661 tgcctctgtc gccaccgccc gatgaaggcg ccggcgacgg cgcataccgg catcgccttc
      721 ttcatcaaca actgtgacgc gcttccaacc ctcgggtggc agcgaatcac ccgctaccat
      781 accagggaaa gctgccctta tcttttcatc cagctcggaa gagaaagccg ctggattttc
      841 acgcgtcagg atatctttga cacgcttcat agcgcgcgca tgtgaatcca accctccccg
      901 ttctatccac tgggcgcgcg gatcacggtc ggcaacctct gccaggagca tggtggtgcg
      961 catgcgttcc agagtatgct cggttccggc gaacatgcct gcaggcccga tctctgcgat
     1021 ctggtccaga gcgaagttct cgcggctaaa atcgaaaccg cgcttgattt gtttcatcat
     1081 cagggcgatc tcgttgtcga tgacagcttt ggcaaagtcg aaagccatca aggcgtctaa
     1141 aagcccgccc atattgaaga tgtccacgcc gcctagatag ccagccacaa ccgacatacc
     1201 cgtctcatat cccgactggg catcgttaat tttggagttc gtcaggccga tatatccgcc
     1261 agaagggata ttataaaagc gtgccatctg ggcacatccc atatgcagga tgccggactc
     1321 aatggcgccc ggcgagtagg ctccggtgcg catatcagcc accgtgggca aggtgctgta
     1381 gatgacggcc ttaccaggct ggatcatctg ggtgagcaca gccgccccca gaaactcagc
     1441 gttacactgc gccaacgtag ctagcatggt cagcggtgag gtgagcccag cgttaggcac
     1501 gacggagaaa tagctgggca aattcttctc gctaaagtag atcaaaagct gggtagagtc
     1561 gaaatccatg gtcaatggcg acaccaccgg gcagtagtga tgggtgatga aaggacgctc
     1621 gcggaaggcg tcttccccac cagccaccag ataagccagg ctcaacatca tatccgcctc
     1681 agcaggggga gtgttggccc gcacgggctt gaccgtgttt ttgatagaag gataatagcg
     1741 ggaaaggctg aattgacctt taggagcatc gtccgccagc gtagagatgg agaagacatc
     1801 gtaaccgtct agctcgttaa tcagatggga tatacgcgcc aggtcatcag agcgggaacg
     1861 ccgctcatat cccgtaaccg gatcgatgat attcggggcc gagctgccgg tgaggatcag
     1921 ggggctgtcg tcggggagcg tacgatcata gcgcgggtcg cgagcccgga atgtgaactt
     1981 ggggggaaac aagacgcggt aacgctcaac aacctggcgg gggatgcgca caatctgggt
     2041 ttgctcttcc aattgcgcgc cgtgggcagc gaagatctcg cgggcgcgtt cattgcgcac
     2101 cagcatgccc actcgctcta ggatctccag cgaggcctca tgcacttgct ccacttgttc
     2161 ttgagaaagc agtgccgcaa aagaatgaat catggtttat cctccatcga gagtaatctc
     2221 agatcaaatg gcgtcgcttc ggtgacaaag cggaagcgat cgccgcgata gcggtcttta
     2281 ccgtactcga ccggtcgatc atctacatca tatgagatgc gcttctccag catgagcggc
     2341 gcgcccacgc gcaccgccag caattctgct tcaaaggccg aggccacgac aggctcgaaa
     2401 ctctggcggg cgcgagcgat agtcacgccg tactcgcttt ccatcacttc gtacacagaa
     2461 cggctttcca ggtcatgggc ttcaaggcct gggaagcgag gtgccgggag cgtgtagctc
     2521 tccagcaaaa ccgcctcctg attgaccgtg cgcacccgcc ggatgcgata agctggcaaa
     2581 gagatcggca ggttcagctc gcgggcggtg gctgcgtcca ggaaagtctg ttccagggaa
     2641 attaaccgtg taccaggagt atacccgcgg ttctgcatgc cgcgcgtgaa acggaagacc
     2701 gagtccatac gccggtctat cttaggctca gcgataaatg tgcccacccc gtgcctgcgc
     2761 acaaccagac cctgggattc cagcacccgc agggcgcgtc gcaaagtcat acggtttacc
     2821 cctagtttct cgctcagctc gcgttcggac ggcagctggt ctcccggggc taattcaccg
     2881 gattcgacct gactgatcag cccctcagcg atctgaatgt acaggggaat ggaataggca
     2941 agcgaatgtg tcgggtaggg tgcaatcata cctatctcat aagtggagta gtggtataca
     3001 catcttatac cacacattat actacaaatg atctcattta ccaatactca tagaagttac
     3061 gcagttgggg aggtgatggt cctgcctttt cgcattccgg cggacaagat gcgatgatat
     3121 gattattacc gcatccaaat aatcccggag ataaaatggc taccctaccc ctgatccaat
     3181 atgatgaggc ttctgcccag gtgaaagctg tcttcgacga gatcaaacaa gcccgccaaa
     3241 ttgatgacgt caacaacttc tggaaagcct tagcaaacca cccagata
//
LOCUS       DBNHOH010000170         4611 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_137901, whole genome shotgun sequence.
ACCESSION   DBNHOH010000170 DBNHOH010000000
VERSION     DBNHOH010000170.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4611)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_137901"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="AC16V7_03030"
     CDS             complement(<1..1307)
                     /locus_tag="AC16V7_03030"
                     /inference="COORDINATES: protein motif:HMM:NF016155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HIR6256123.1"
                     /translation="MTYFRVLAVFLIPPLLGLLAIVPTDVWRWLRDRSTLVHRRAYIV
                     ILIHVLLALVYTTPWDNYLVATGVWTYPPELVSGVILGWVPLEESLFFILQTLTTGLW
                     ALAMRRIFPRKQARIKQASAMRRLGTAILALIWVIWLIILLSGWRPGTYAALILVWGL
                     IPVLIQVAFGADILFANGRALVAAILPTTMYLWLVDMLAIRSGTWTIDLGQSTGLMIA
                     GLPLEEMLFFLMTNVIIVFGVNLMLDEESQTRARDLKQWWQNFTHITPREGEKIQSLW
                     LVRSPRVLGLWISLVLWIVILIATPIAMWLGGEGLFPLMASLGVLAQCSATLFSMYQG
                     WAFRKVAGVALIVLVLAWMIEWLGGTTGFPFGDYHYTEILRPKLGNVPWLIPIAWLMM
                     LVPSWAVGQAILGGIRERLKGLYWVTLTVISGAAFTAWDLYLDP"
     gene            complement(1304..2125)
                     /locus_tag="AC16V7_03035"
     CDS             complement(1304..2125)
                     /locus_tag="AC16V7_03035"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6256124.1"
                     /translation="MTLNLRPYSGAEHQPFEMGDGKSGFLLLHGFTGTPAEVRGLGET
                     LAQLGWHSHAPLLPGFGSDIVNLDQRRRADWLDAARDVWEKLHHRYQPRVLFGYSMGG
                     AIAIHLACELSPDILVLAAPFWRMPGWMPRLIPAARIFTPHMRPFKKADFSDVRLRRE
                     FERIMPGIDLDDTEVQESIRAEFTLPLTALHEAMKLGSSAYKLAKRVQCPVLVLQGEQ
                     DTIVRSDQTRKLFRRIGSPRKSYQSIPTGHDLLMAGTPQLEQTVNTVLGFIESIR"
     gene            complement(2115..2591)
                     /locus_tag="AC16V7_03040"
     CDS             complement(2115..2591)
                     /locus_tag="AC16V7_03040"
                     /inference="COORDINATES: protein motif:HMM:NF047191.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6992 family protein"
                     /protein_id="HIR6256125.1"
                     /translation="MTRFPTDKRAFNLFRFQMEALPVLMGWAAGSIAAGLLWLRSKSA
                     VMAGIGGQFVLWGAIDGLIAALGLRGAAGGMRRLAKKEMSEADHLQQAERFERFVWLN
                     AGLDVLYIMGGGWLAQRSRNDDRRRGMGIGIVLQGGFLLVWDVILALLVRGKRRDA"
     gene            complement(2594..3538)
                     /locus_tag="AC16V7_03045"
     CDS             complement(2594..3538)
                     /locus_tag="AC16V7_03045"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HIR6256126.1"
                     /translation="MDVDVNLSLPTVRSVREGEINSFKESGSIASVADYAACRAIMLR
                     ASNNYSSASRYLPADKLLHVEALYALMRVGDDRVDVSHQGFRTPLEAIEDWEKTYWLA
                     FERGESPHPVMRAYLDTAIKFGIPAETMSAYFRAMKEDLTVTRFPTFNHLLHYMEGSA
                     IPVGRAMTHILGVREPYSIGEALPGADSLSTAMQLSNFWRDIGEDWAIDRVYLPLEDL
                     QRFGYSLSDLGAQRIDERLIALLEFQFERTEKYYQRARTSVDMLNSGQWAVMTALEVY
                     RALLGDIRSHGYNVFSRRAGTSNLKKIGLAIKAYWAIR"
     gene            complement(3529..>4611)
                     /gene="crtI"
                     /locus_tag="AC16V7_03050"
     CDS             complement(3529..>4611)
                     /gene="crtI"
                     /locus_tag="AC16V7_03050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR6256127.1"
                     /translation="GMDFVDRNYDQLTDLMNPSAAVRLLQTQAHQNLYQQVSAFFQHN
                     DKLAKAFSFHSMFLGLSPFDALAMYSLITYADLALGMWFPMGGLYRLIEDMVSLAGEM
                     GVEIRTESPVEEITIVDGRVTGVRLESGEQVASDLVISNADLPYTYNKLIAPRYRKQY
                     SSARLAKMQYACSGYILYLGVNRIYPHARHQALYFSEDYRANLDAIFKHKTLPEDPSF
                     HLNIPTVTDPNLAPPGHSLIYVLAPMPNLEAKIDWQQAAPVVRQRLLDRLEQILDPEI
                     RQHIVWERDYRPTDWHSDINATLGTAFGSLAHGFFQSAYFRPHNKAKGVQGMYFVGQG
                     TYPGIGMPMVVISSKLVTERVLKEWM"
BASE COUNT         1192 a         1317 c         1095 g         1007 t
ORIGIN      
        1 gggtcgaggt acaggtccca tgcagtgaaa gctgccccag aaatcacagt caatgtaacc
       61 cagtataaac cctttaagcg ttcacgtatc ccacccagga tagcctgccc cacagcccaa
      121 gatggcacca gcatcatcaa ccaggctatt ggaatcagcc aagggacatt accgagtttg
      181 gggcgaagga tttctgtgta gtgataatca ccaaatggaa accctgtcgt cccacccagc
      241 cactcaatca tccaggccag gacgagtaca atcaaggcca ccccagcaac cttgcgaaaa
      301 gcccatccct ggtacatgga gaataacgtc gcgctgcact gagccagcac acccaggctc
      361 gccataagcg ggaataggcc ttctccgcca agccacatgg cgataggggt ggcgatcagg
      421 atcacaatcc atagcaccaa gctgatccac aatccaagta cgcgtggtga cctaaccaac
      481 cacaacgatt ggattttttc accttctctt ggtgttatat gagtaaagtt ctgccaccac
      541 tgctttagat ccctggcgcg tgtctggctt tcctcgtcca acattaaatt taccccaaaa
      601 acaatgatca cattggtcat caagaagaag agcatttcct cgaggggaag tcctgcaatc
      661 atcaagccag tagattggcc caggtcaatc gtccacgtgc cactgcggat ggcaagcatg
      721 tccaccagcc acaaatacat cgtggtcggt aagattgccg caacgagcgc ccgtccattt
      781 gcgaacaaga tatccgcccc aaacgcgacc tggataagga caggtatcag cccccacacc
      841 aggatcaacg ctgcatatgt gcccggacgc cacccagaaa gcaagataat aagccagatc
      901 acccaaatca atgccaaaat ggctgtaccc agcctgcgca tcgctgaggc ttgctttatc
      961 ctcgcctgtt ttcgcgggaa aatccgcctc atggctaatg cccacaatcc ggtggttagg
     1021 gtttgcaaaa taaaaaacag agactcttca agcggcaccc accccagtat caccccactg
     1081 accaattcag ggggataggt ccacacgcct gtggcgacca ggtaattatc ccatggagtg
     1141 gtatagacta aagccagcaa tacatgaatg agaataacta tataagccct gcgatgtacg
     1201 agggtcgaac ggtcgcgcag ccagcgccac acgtctgtag gcacgatcgc tagcaaaccc
     1261 agcagcgggg gtatcagaaa aacagccaga acgcgaaaat aagtcatcga attgattcga
     1321 taaatccgag cacagtatta accgtctgct ccaactgcgg tgtgcccgcc atcaagagat
     1381 cgtggccggt gggaatcgat tggtagcttt tacgaggcga accgatgcgc cgaaataatt
     1441 ttcgtgtttg atcagagcgg acgatcgtat cctgttcacc ctgcaagacc aaaaccgggc
     1501 attgaactcg ctttgccagc ttgtacgcgc tgctcccaag tttcatagcc tcgtgtaagg
     1561 cagttagtgg aagggtaaac tccgcccgga tagattcctg cacttccgta tcgtcgaggt
     1621 caattccagg catgattcgc tcaaactcgc gccgaaggcg gacatcgctg aaatctgctt
     1681 tcttaaatgg acgcatatgt ggggtaaata tccgcgctgc agggatcaac ctcggcatcc
     1741 agccaggcat gcgccagaaa ggagcggcta gcaccaggat atcaggggat aattcgcaag
     1801 ccagatggat tgcgatcgct ccgcccatcg aatatccaaa gagcaccctc ggctggtatc
     1861 tgtgatgcaa tttttcccac acatcacggg ctgcatccag ccagtctgcg cggcggcgct
     1921 ggtcgagatt gacgatgtcg ctgccaaaac ctggcaggag cggtgcatgg gaatgccaac
     1981 ccagctgggc aagtgtctcc cccaggccgc gcacctcggc gggtgtgccc gtgaagccat
     2041 gcaacagcaa aaatccggat tttccgtcac ccatctcaaa gggttggtgt tccgctccac
     2101 tatagggtcg caggttaagc gtcacgccgc ttgcccctca ccagcaaagc caggattaca
     2161 tcccatacca gcaaaaatcc accctgtaac acaataccta tgcccatacc gcgtcgtctg
     2221 tcatcgttgc gactgcgttg cgccagccag ccacccccca taatatacaa cacatccaat
     2281 cctgcgttta accatacaaa gcgctcaaag cgctctgctt gctggagatg atctgcttcg
     2341 ctcatctcct tcttagccag acgccgcatt cccccagccg cgccgcgcaa acccaaggct
     2401 gcaatcaatc cgtcaatcgc tccccaaagg acgaattgac cacctatacc agccatcact
     2461 gcgctttttg agcgcaacca gagcaaacca gctgcaattg agccagctgc ccaacccatt
     2521 aatacaggta gagcctccat ctggaaacga aacaggttga aggctcgctt gtcagtggga
     2581 aatctagtca tgatcagcga atcgcccaat aagcctttat cgccaagccg atttttttga
     2641 gattgctggt tcctgctcgt cgcgagaaca cattgtatcc gtgagagcgt atatccccta
     2701 agagcgctcg gtacacttcc agagccgtca taaccgccca ttgccctgaa ttcagcatat
     2761 ccacgctcgt gcgggcgcgt tggtaatact tttcagtacg ctcaaattgg aactccagca
     2821 aggcaattaa ccgctcatca atgcgctgtg cacccagatc cgagaggctg tatccaaagc
     2881 gctgtagatc ttcaagtggt aagtaaacgc ggtctatcgc ccaatcctcg ccaatatctc
     2941 gccagaaatt actcaattgc atggctgtag acaggctgtc tgcacctggc aaagcctcgc
     3001 cgatagagta tggctcacgt actcctaaaa tatgagtcat tgcccgccct accggtatag
     3061 cgctgccctc catgtaatgc agaaggtgat taaacgtggg gaagcgcgtt acggtcagat
     3121 cttccttcat ggcgcggaag tatgcgctca tcgtctctgc agggatgcca aattttatcg
     3181 ccgtgtccag ataggctcgc attactggat gtgggctttc gccccgctca aacgctaacc
     3241 agtatgtctt ttcccaatct tcaatcgcct ccaacggtgt gcgaaatccc tggtgtgata
     3301 catccacgcg gtcatctccc acgcgcatca aggcatacaa cgcttcgaca tgcagaagct
     3361 tatcagccgg caaatacctg ctggcggatg aataattatt actcgcccgg agcataattg
     3421 cgcgacaagc cgcataatct gctacagaag cgattgagcc actctcctta aagctattga
     3481 tttcgccttc acgaactgat cgcacagtcg gtaatgataa attgacatct acatccattc
     3541 tttcagcacc ctttctgtaa ccaacttcga agagatcacc accatcggca tgccgatccc
     3601 cggataagtc ccttgaccta cgaagtacat cccctgcaca cccttagcct tgttatgtgg
     3661 gcgaaaatat gcggattgaa aaaagccatg tgctagcgag ccaaatgccg tccccaaggt
     3721 ggcattgata tctgagtgcc aatctgtggg ccggtaatcc ctctcccaga cgatatgctg
     3781 tcggatctcg ggatccagga tctgctccaa tctgtccagc agtcgctggc ggacaactgg
     3841 cgcggcttgt tgccagtcga tttttgcctc caggttgggc atgggcgcca gcacatatat
     3901 taagctgtgc ccaggaggcg ctaaattggg gtctgtgacc gttggaatgt tcaggtggaa
     3961 cgagggatct tctggcaaag tcttgtgttt gaagatagcg tccagatttg cacggtaatc
     4021 ctctgagaag tatagagctt ggtggcgggc gtgagggtag atccggttaa ctcctaggta
     4081 taaaatgtaa ccagagcaag cgtactgcat tttcgccaac cgagcggaac tatattgctt
     4141 gcggtagcgg ggagcgatca atttgttata cgtgtagggg agatcggcgt tcgaaatcac
     4201 caggtcagat gccacttgtt ccccagattc caaacgaaca ccggtcactc gaccatccac
     4261 aatggtgatt tcttctactg ggctctcggt gcgtatctcc acccccatct cacctgccag
     4321 actgaccata tcttcgatca aacgatataa gcctcccatg ggaaaccaca tacccaatgc
     4381 cagatcggca tacgtaatca ggctatacat cgctaacgca tcgaatggtg agagccccag
     4441 aaacatcgag tgaaaagaga aagccttggc gagtttatca ttatgctgga agaaagccga
     4501 cacctgttgg tacaggtttt ggtgcgcctg cgtctgcaac agacgaaccg cagcagatgg
     4561 attcatcaag tcagttaact gatcataatt gcggtctacg aaatccatac c
//
LOCUS       DBNHOH010000171         4414 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1676965, whole genome shotgun sequence.
ACCESSION   DBNHOH010000171 DBNHOH010000000
VERSION     DBNHOH010000171.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1676965"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="AC16V7_03055"
     CDS             <1..272
                     /locus_tag="AC16V7_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256128.1"
                     /translation="ADTSGDDLVSVGEGLPYYGPVLLALQPFTTAPGGVIDFEATYTE
                     LSNLAPINALQNRTIVIHGKTVGDDYIGSLPVACGQIRPEPQGNK"
     gene            435..830
                     /locus_tag="AC16V7_03060"
     CDS             435..830
                     /locus_tag="AC16V7_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256129.1"
                     /translation="MHLVKARVLIGVVFIVNVQCAVAFLLFPGWYAPLFELQGPAGEA
                     MIQGMGVLFLMWNVPYGVALWNPIRHRRALFEAVFMQAIGLAGETWIYLTSASALVIA
                     REALTRFIIFDALGLLALIAAVWLTRGKR"
     gene            complement(952..1308)
                     /locus_tag="AC16V7_03065"
     CDS             complement(952..1308)
                     /locus_tag="AC16V7_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013920.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR6256130.1"
                     /translation="MKREIDITAYKCKKCGKLHYPFHDRCLGCKTREFEKVKPQGNTK
                     LLTFTRIYNLPWGFDQRYLTIGVVEFENGVRAMGQINVDETYNLKIGMKMRPSWEPVR
                     VQYGEDVYGLMLEPMG"
     gene            complement(1305..2495)
                     /locus_tag="AC16V7_03070"
     CDS             complement(1305..2495)
                     /locus_tag="AC16V7_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase C-terminal domain-containing protein"
                     /protein_id="HIR6256131.1"
                     /translation="MRPVAVIGVGMIPFGRRDEDSLMDMLAYASLNALDDAGLGDKTV
                     DAVYVGNMGGGIIQHQTSIASSLVDRLSLLPAAAETVENGPASGGSAVKNGLLAVASG
                     YYDTVLVVGGEKMRETTGWRATDFVSTMTHPLAEYVYGITLPGLAGMFTRLYMEKYGV
                     TREHLIAIAIKNQEMGGLNPYAHVEMSITKEGIYDNSASIVNNPVVADPLHLYDACPV
                     SDGAAALILCPLDKIKNPAKTPVVIAGFGQATDTHTLQEREDPTDLKAVTLASQKALE
                     MAKLKPSDIHVAELHDAYTILEVAESEHVGFFKKGEGGKAALAGKTALGGTIPINVSG
                     GLKARGHPVGATGVAQVVDIVYQLRHELPKNRQVKNAKNGLTVNFGGFGNNVLSFVLR
                     RVEQ"
     gene            complement(2500..3189)
                     /locus_tag="AC16V7_03075"
     CDS             complement(2500..3189)
                     /locus_tag="AC16V7_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256132.1"
                     /translation="MAIDMELEAVLRPHQYEMPPYPPKVITLANSKKMVVRQIQREDV
                     PTLLKSVFPCLTIERDYYDIVSARLYGELLAWYRYRVKSEYCLVGQIDGYAVGIVNGR
                     METEKVGMSYHTLAIDRGLRIGSHLFAAKMEYHIEYLGQDEVLIVAESPIGFRRWMIE
                     YPLEKTDIPHELGGGDSYLLTRDIYLKAKPRLVVGERPVPKELMEEAEADILVVDEDV
                     IREKIAGLKGR"
     gene            complement(3211..4092)
                     /locus_tag="AC16V7_03080"
     CDS             complement(3211..4092)
                     /locus_tag="AC16V7_03080"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR6256133.1"
                     /translation="MKFIDLTIPLGVATPPWPTYEPLQVKYFKRLAPNGANGQLVTHS
                     NHLGTHLDGEIHFHTPGKDIASLEMDFLVHEAAIVDLSDICGDYDVYTSKMVEERVEV
                     REGDILIIHTGYHHFGWDMPSANEVRYMVMHPGPDREFADWAKAKKLRWIGVDCGSAD
                     HPMNTIIRDWMPRQARQAEKVFKKKFGKSLAEYFDDTKYQLMHLEMFPYGIIHAECLG
                     GEIDLLVNRRVQVGFFPWRFVDGESSIGRCVAIVDDVEYEQLMQKKAQLPKNRFGDAY
                     DPTHLERINQLSALNLS"
     gene            complement(4168..4293)
                     /locus_tag="AC16V7_03085"
     CDS             complement(4168..4293)
                     /locus_tag="AC16V7_03085"
                     /inference="COORDINATES: protein motif:HMM:NF024759.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HIR6256134.1"
                     /translation="MDIEAVAKTIIDAGYRVDMIVENCILIENKTVDQLAPIHEA"
BASE COUNT          976 a         1190 c         1116 g         1132 t
ORIGIN      
        1 cagcggacac gagtggagat gacttagtga gcgttggaga aggcctgcca tattatggtc
       61 cggtcctttt agcgcttcaa ccgtttacga cagctccagg tggcgtaatc gattttgaag
      121 cgacctacac agaactcagc aatcttgccc cgatcaatgc tttacagaat cgtactattg
      181 tgatccacgg gaagactgtt ggtgatgatt acatcggctc cttgccggtt gcctgcgggc
      241 agataaggcc agaaccgcaa ggaaacaaat aatagattaa gaccagcttt ccctattggt
      301 ttggggagcg ctcaacaaaa tgaactatcg ggcggtaata tcaccgcccg atagccattt
      361 aacttcactc ttgggtgttt aagccatagt ctcgttcatg cagtacaagc agaaattacg
      421 gatacaattg gcctatgcat cttgtcaaag cccgggtctt gatcggagtt gtttttatcg
      481 taaacgtcca atgcgcggta gccttcctgt tatttccagg ctggtatgcg ccgttatttg
      541 agctccaggg tccagcaggc gaagcgatga ttcagggcat gggtgtgctg ttcctgatgt
      601 ggaatgtgcc ctatggcgtg gcgctctgga atccaatccg ccaccgccgg gccttgtttg
      661 aagcggtctt tatgcaggcg attggtctgg caggagaaac atggatttat ctgacaagcg
      721 catctgcact ggtaatcgcc cgtgaggcgc ttacccggtt tatcattttc gatgccttgg
      781 gattgctggc actcatcgca gccgtatggc tgactcgggg caaaagatag atgagcaagg
      841 gtcaagaatg gtaatgtgta actggaaaac tgtgtttaat ggggactgcc agccctgatt
      901 tatatgggct ggcagtcgtt ttctgatcga ataggttgaa ttgttgccga attaacccat
      961 tggctccaac atcaacccat acacatcctc gccgtactgc acacggacag gctcccagga
     1021 tggtctcatc ttcatgccga tcttcaagtt ataggtctcg tcgacgttta tctgtcccat
     1081 ggcgcgcaca ccgttctcga attcgactac cccaatggtc aggtagcgct gatcgaaccc
     1141 ccagggcagg ttgtagatgc gggtaaaagt gagtaacttc gtatttccct gtggtttcac
     1201 tttttcaaac tccctggtct tacaccccag gcagcggtcg tgaaaggggt agtggagttt
     1261 accgcatttc ttgcatttat aggccgtgat gtcgatttct cgcttcattg ctctaccctc
     1321 ctcagtacaa aggacaggac attattccca aatccaccaa agttgactgt caggccattc
     1381 ttagcgttct tgacttgcct gttcttgggc agctcgtggc gcaattggta aacgatatcc
     1441 actacctggg caacgccagt cgcgccgacc gggtgaccgc gcgccttcaa cccgccagag
     1501 acattgatgg ggattgtccc acctagcgca gtcttgcccg ccagtgccgc cttgccccct
     1561 tcgcccttct tgaagaatcc aacgtgctcg ctttcagcca cctccaggat ggtgtacgcg
     1621 tcgtgcagct cggccacgtg gatatccgat ggtttgagct tggccatctc taatgctttt
     1681 tgggaagcca gagttaccgc ttttaggtcg gttgggtctt cgcgctcctg caaggtatgg
     1741 gtatcggtag cctgaccaaa cccggcgatc actaccgggg ttttggcagg atttttgatt
     1801 ttatccagtg ggcagaggat caacgccgca gcaccatcgc tcactgggca ggcgtcgtat
     1861 aggtgcagtg ggtcagccac cacggggttg ttgacgatag acgccgaatt atcgtaaatg
     1921 ccctctttgg tgatggacat ctccacgtgt gcgtacggat tcagcccgcc catctcctga
     1981 ttcttgatgg ctatggcgat aagatgctca cgggtgacgc catatttttc catgtacagg
     2041 cgggtgaaca tgccagccaa ccccggcaag gtgatgccat aaacatattc agccagcggg
     2101 tgggtcatcg tggatacgaa atcggtagcg cgccagcctg tcgtctcgcg cattttctcg
     2161 ccacccacca ccaagacggt gtcataatag ccagaggcta ccgccagcaa gccattctta
     2221 actgccgaac cgccagaagc agggccgttc tccacggttt cagcagctgc gggcagcagg
     2281 ctcaagcgat ctaccagcga gctggcgatc gaagtctggt gttggatgat gcctcccccc
     2341 atatttccta cgtacactgc gtcgacggtt ttatctccca ggccggcatc gtccagcgca
     2401 ttcagagagg cgtaagccag catatccatc aggctgtctt cgtcacgccg cccaaagggg
     2461 atcatgccaa cgccgataac agctacgggt ctcattgttt catctccctt tcaatccggc
     2521 aattttttcg cggataacat cttcatcgac aaccaggata tctgcttcag cttcttccat
     2581 aagctccttg ggcactgggc gttcacccac caccaagcgt ggtttggctt ttaagtagat
     2641 atcacgcgtt agcaggtatg agtccccacc acccagctca tgcgggatat ctgtcttctc
     2701 gagcgggtat tcgatcatcc agcgccggaa gccgatgggg ctttctgcta cgattaggac
     2761 ttcatcttgc ccaaggtact cgatatggta ttccattttt gcggcaaaca gatgtgagcc
     2821 gatgcgcaag ccacggtcga tcgctaatgt atggtagctc ataccgactt tctcggtctc
     2881 catgcgccca ttaacaatcc ccaccgcgta gccatcgatt tgcccgacaa gacagtactc
     2941 actttttacc cgatagcggt accaggctag cagctcgccg taaagccgtg cgctgacgat
     3001 gtcgtaatag tcgcgctcaa tggtcaggca ggggaaaaca gacttgagta acgttggtac
     3061 atcctccctc tgtatctggc gcaccaccat tttcttgcta tttgccaggg tgatcacctt
     3121 gggcgggtag ggcggcattt catattgatg cggtcgcaag actgcttcca gttccatatc
     3181 aattgccaca gtaaaacctc ctttaattga ttagctgaga tttaaagcac tcaactggtt
     3241 gattcgctct aaatgcgtgg ggtcgtaagc atccccgaag cggttcttgg gcagttgagc
     3301 ctttttttgc ataagttgct catattctac atcatctacg atagccacgc agcgaccaat
     3361 ggaggattct ccatccacaa agcgccaggg gaagaacccc acttgcacgc ggcgattgac
     3421 gagcaggtct atctcgccac ccaaacactc ggcgtgaatg atgccatagg ggaacatctc
     3481 caggtgcatg agctggtatt tggtgtcatc gaagtactca gccagtgact tgccaaactt
     3541 cttcttgaat accttctctg cctggcgagc ctgtctcggc atccagtctc gaatgatggt
     3601 gttcatcggg tggtcggcgc taccgcaatc gaccccgatc cagcgcagct ttttggcctt
     3661 ggcccagtca gcaaattcgc ggtctggacc agggtgcatc accatatagc gcacctcgtt
     3721 cgcagagggc atgtcccagc caaagtgatg gtagccagta tggatgatga ggatatcccc
     3781 ctcgcgaact tcgacacgct cttcaaccat tttgctggtg tatacatcgt aatcgccgca
     3841 gatgtcgctc agatcgacga tggcagcctc gtgaactaaa aaatccatct cgagcgaggc
     3901 gatatccttc ccaggtgtat gaaagtggat ttcgccatcc aggtgggtgc ccagatggtt
     3961 agagtgggta actaactgcc cattcgcccc atttggcgcc aggcgcttga agtacttcac
     4021 ctgcagaggt tcgtacgtgg gccagggagg ggtagccact cccaggggta tggtaaggtc
     4081 aatgaatttc atgctaaagc tccttatata tattttttca atcttcaccg cgaaggcgct
     4141 atgagcgcga agattcttct ataacaatta ggcctcgtga atcggagcaa gttgatcgac
     4201 cgttttgttt tcgataagaa tgcaattctc gacaatcata tccactctgt agcccgcgtc
     4261 tattattgtc ttggcaaccg cctcaatatc catagatgcc ttcgcgttct ttgcgcctta
     4321 gcggtttatt acagaatcct caatgtttca aacagaatac cgatctgcat cacaccgcct
     4381 ttactccttg acgtaaggct ggccaagcgc ttca
//
LOCUS       DBNHOH010000172         4356 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_523091, whole genome shotgun sequence.
ACCESSION   DBNHOH010000172 DBNHOH010000000
VERSION     DBNHOH010000172.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_523091"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            324..1691
                     /locus_tag="AC16V7_03090"
     CDS             324..1691
                     /locus_tag="AC16V7_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256135.1"
                     /translation="MDGSNNLKLKDGSRVAVIGGGPAGSFFSYFLLQFAERMGLEIFV
                     EIYEPRDYSKPGPTGCNMCAGVVSESLVQNLATEGIILPSTVVQRGIDSYVLHMDVGS
                     ALIPSIRDERRIGTAFRGVGPRGIKEIKWDSLDHFLMKLALDKGAQHIPARVSEVEKV
                     ADPEKLNGGGQRLQVKTTKGLTQTYDLLAVSAGVNTAILKLFQGMDFGYQPPLTTQCF
                     VREYYLGEEAVSKHLGSSFHAFLLNIPNLDFAAMVPKGDYASLALLGENIDADLLQTF
                     TSDPALERCMPPNYPMDQVDCWCSPRINIKGSARPYGDRIVFIGDSAVSRLYKDGIGA
                     AYRAAKAAATAAIFEGVSSEAFERRYWPACRSMEIDNTIGKILFAVVREVQKRKFARR
                     AILQMVLSEQKRGVVGDRNMSMVMWDMLTGSAPYRKILLRTLHPVFWMRLLKHFFVAA
                     VQSFG"
     gene            1756..2172
                     /locus_tag="AC16V7_03095"
     CDS             1756..2172
                     /locus_tag="AC16V7_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR6256136.1"
                     /translation="MMKTGELGKVYQDRELIIRQGEAGDCMFVIQQGQVEILSEKDGK
                     EIRLAVRGEGEFIGEMAIFEREVRMATVRALGEVRVLTVDKKNILQRIHEDPSLAYRM
                     IQTMSRRIREQSQEVVRLTNLIKEKGALLDNDVPHR"
     gene            2156..>4356
                     /locus_tag="AC16V7_03100"
     CDS             2156..>4356
                     /locus_tag="AC16V7_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6256137.1"
                     /translation="MSPIAERYPSTSSSLPTGTLTYLFTDVEGSTRLWEQYPDQMRGV
                     MIRHDDLIESLVTNYGGSVVRPRGEGDSRFAVFPRAADAVAAASAIQRALSFEAWIIP
                     SPLRVRMGLHTGAADLRDNDYYGPAVNRCARLRNAARGGQVLVSQNTNVLVRDALPEG
                     VSMRDLGEYRLKDLNRTEHIYQLVIEGLPGDFPPLKALENLHTNLPAALTSFVGRERE
                     IEEVKQLLTQTRLLTIAGVGGAGKTRVALQVAEDLLEFFGDGVWWMDLAPISNPELII
                     QFVADVLGIREEAGKSLLQLLTSYMRTKNTLLVLDNCEHLIQGVALFVETILRSAPEL
                     RILATSREALGMSGEVIWRIPPLSAPGLRERVKSENLVQYEAVKLFVERAVAVKADFE
                     ITVENARSVAKICARLDGIPLAIELAAARVRVLSVEEIAERLDDRFRLLVSGNRTALP
                     RQQTLRALLDWSHELLSEHEQILLRRLSIFTGGWTLPAAEKVCFDEEVEAWQILELLT
                     HLIDKSLVVAESHDRSERYRYLETIRQYAQERLLEAKEVDELACKHAEYFLNLAEEAY
                     SELWGPRQATRLVQLEAEHENLRMALEYMSKDGSRAEMLLRLGSSLWRFWEIRGYISE
                     GRAWLKRALDANPDAPAYLRGNGLRGAGILACQQGDYMQAKALHEQSLTLFRELGNKL
                     GIARELNDLGEIARYLGNYQEAVALHTESLALRYEIDDKEGLAVSLGQLGT"
BASE COUNT         1013 a          997 c         1251 g         1095 t
ORIGIN      
        1 gtatgggcgg gcatcgcccg cccatacccc cctcctttcc tcttcaactg cgtaagtcct
       61 ataattattg agtattgcaa ccagaataat ctcaatggaa gctcttgtag aaacccggct
      121 aaatccaaga tggtacactt atctaattcc ttgttcaaga cctgtatccc gattacgtgt
      181 catggtggtt tcgaaagtgg gaagtgtcca ttctatttgc aatacttcta ctaacgattt
      241 tgagaagttg attaattaaa ctttctcaga tgcttaagtt catggcataa tgaagtatgg
      301 ttgcggtaaa gggagcgata gacatggacg gtagcaacaa tctcaagttg aaggacggat
      361 cccgcgttgc ggtgattggt gggggaccag caggctcttt ttttagttac ttccttttgc
      421 aattcgcaga acgcatgggt ttggagatct ttgtggaaat ttacgagccc agagattatt
      481 ccaaacctgg tccgaccggc tgcaatatgt gtgctggcgt ggtgtctgag tcacttgtcc
      541 agaatttggc aaccgaggga ataattctgc cgtccacggt ggtgcagcgc gggattgact
      601 cctatgtgct gcacatggat gtgggcagcg cactcatccc ctccattcgt gatgagagac
      661 gcatcgggac tgcgtttcga ggcgttggcc cgcgagggat taaggaaatc aagtgggaca
      721 gtctggatca ctttttgatg aagctggctt tggacaaggg tgcccagcac atccccgccc
      781 gggtaagcga agtcgagaaa gtcgctgatc ccgaaaagtt aaacggaggt ggtcagcgac
      841 ttcaggtgaa aaccaccaaa ggattgacac aaacttatga cctgctggct gtttctgccg
      901 gggtgaatac cgccatcctt aaactcttcc agggtatgga ttttggctac caaccccctc
      961 tcacaacgca gtgctttgta cgtgagtatt acttgggtga agaagccgta tcgaagcatt
     1021 taggctcatc gtttcacgct ttcctgctga acattcccaa tctagatttt gctgccatgg
     1081 tccctaaagg tgattacgcc tctcttgctt tgttgggtga gaatattgat gcagatcttc
     1141 ttcaaacctt tacgagcgat ccagcactag agcgctgtat gcctcccaac taccctatgg
     1201 atcaggtcga ttgttggtgc tcgccacgca ttaatattaa gggtagtgcc cgaccttatg
     1261 gagaccggat tgtcttcatt ggcgattctg ctgtgagccg tttgtacaaa gatggtattg
     1321 gggcagctta ccgcgcggcg aaagctgcgg cgacggcggc gattttcgag ggcgtatcgt
     1381 ctgaagcttt cgaacggcgt tattggcctg cttgccggtc gatggaaatc gataatacca
     1441 tcggaaaaat tctgtttgct gttgttcggg aagttcagaa acggaaattt gcccggcgag
     1501 cgattttgca gatggtatta agcgagcaaa agcgcggggt ggtcggagac cgcaacatga
     1561 gtatggtgat gtgggatatg ctgaccggta gcgcgccata tcgaaagatc ctcttgcgca
     1621 cgttgcaccc tgttttttgg atgcgtctat tgaagcattt cttcgttgcg gctgtacaat
     1681 catttggctg atggcgtaca attaattcgt gttgctagag aaccagcaac accatcctga
     1741 tcgtgagatg agggaatgat gaaaacaggt gaattgggaa aagtatatca ggatagggag
     1801 ctgatcatcc gacagggtga ggcgggggat tgtatgtttg tcatccagca agggcaggtt
     1861 gaaatcctga gtgaaaaaga cggaaaagaa attcgcctgg ctgtgcgcgg tgagggggaa
     1921 tttattggcg agatggcgat ttttgaacgt gaggtgcgca tggcgactgt tcgggcgctg
     1981 ggtgaggttc gcgtattgac agtggacaag aaaaatattc tccaacgaat ccacgaggac
     2041 ccctcactcg cataccgcat gattcagacc atgtcgcggc gcatacgcga gcaaagccag
     2101 gaagtagtgc gtttgaccaa tcttataaag gaaaagggtg ccctgttaga caacgatgtc
     2161 ccccatcgct gagcgatacc cgtccacatc ttcttctttg cccacgggaa cgctaaccta
     2221 tctttttacc gatgtagaag gtagtacacg cctttgggag caatatccag accaaatgcg
     2281 tggggtgatg attcgtcacg atgacttgat cgagtcgttg gtgacgaatt atggtggttc
     2341 tgtcgtccgg ccgcgtggcg aaggcgatag ccgttttgcc gtgtttcctc gcgctgcgga
     2401 tgctgttgct gctgccagtg ctattcagcg cgctctgtct ttcgaagctt ggataatccc
     2461 ctcacccctg cgcgtgcgca tgggattgca tactggtgcg gcagatttgc gcgacaacga
     2521 ttattacggc ccagccgtga atcgatgcgc cagactccga aacgctgctc gtgggggaca
     2581 ggtgcttgta tcccagaaca ccaatgttct ggtacgagat gctttaccgg agggggtaag
     2641 tatgcgcgat ttaggcgaat accgcctaaa agatcttaat cgaacagagc acatttacca
     2701 attggttatc gagggattgc cgggagattt cccacccctc aaagcgttgg agaacctgca
     2761 taccaattta cccgccgcac ttaccagctt tgtcggtcgt gaacgtgaga tagaagaagt
     2821 gaaacaatta ctgacgcaaa cgcgactgct caccatcgcg ggtgttggcg gggcgggcaa
     2881 aacgcgcgta gcattgcagg tggccgaaga ccttctggaa ttctttggtg atggtgtgtg
     2941 gtggatggat ctggcaccga tatccaaccc tgaactaatc atccagtttg tagcagatgt
     3001 tttaggcatc cgcgaagaag caggtaagtc attgctccag ttgctaacca gttatatgcg
     3061 taccaagaat accctgctgg tactggataa ttgtgagcac ctgatccagg gtgtcgccct
     3121 tttcgttgag acgattctgc gcagtgcgcc tgaattgcgg atactggcca ccagtcgcga
     3181 ggcgttgggc atgtcgggcg aggtcatctg gcggattccg cccctttctg cgcccggctt
     3241 gagggaaaga gtcaagtctg aaaacctggt tcagtacgag gcagtcaaac tttttgtcga
     3301 acgcgccgtg gcagtcaaag cggatttcga gatcacggtt gagaatgcgc gctcggtggc
     3361 aaagatttgc gcacggttgg atggcatacc actggccatc gagctggcgg ccgcgcgggt
     3421 gagggtgcta tccgttgaag agattgctga acgcctggat gatcgttttc gattgctggt
     3481 cagcggcaat cgcaccgctc tccctcgcca gcagaccttg cgtgcattgt tggattggag
     3541 tcatgaactg ctatctgagc acgagcagat tttactgcgg cgactatcga ttttcactgg
     3601 tggctggaca ttgccagcag cggaaaaggt atgcttcgat gaagaagttg aagcttggca
     3661 gattctcgag ctgctaaccc atctgatcga caaatcgctg gtcgttgcgg aatctcacga
     3721 ccgcagtgag cgctatcgct atttagagac gattcgccag tatgcccagg agcgtctttt
     3781 agaagccaaa gaagtggacg agctagcgtg caagcacgcg gagtattttt taaacctagc
     3841 cgaggaagca tatagtgagc tgtggggtcc aagacaagcc acacgcttgg tgcagctgga
     3901 agctgagcat gaaaatttgc gcatggcgct ggaatatatg tcgaaggatg gaagccgggc
     3961 ggaaatgctg ttgcgcttgg gcagttctct atggcgcttt tgggaaattc gtggatatat
     4021 cagcgagggt cgtgcctggt tgaagcgcgc tttggatgcg aatccggacg cgccggccta
     4081 tctgcgggga aacggtctgc gtggggcggg tattctcgcc tgccagcagg gtgattatat
     4141 gcaagccaaa gcgctacatg aacaaagcct aactctcttt cgagaactgg ggaacaagtt
     4201 gggtattgct cgcgagctca atgatttggg ggagatcgct cgttacttgg ggaattatca
     4261 agaagcggtc gccttacata cagaaagcct ggcgttgcgc tatgaaattg atgacaaaga
     4321 aggtctcgcc gtgtcattgg ggcagctcgg tacgat
//
LOCUS       DBNHOH010000173         4450 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_586886, whole genome shotgun sequence.
ACCESSION   DBNHOH010000173 DBNHOH010000000
VERSION     DBNHOH010000173.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_586886"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="AC16V7_03105"
     CDS             <1..544
                     /locus_tag="AC16V7_03105"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF014670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR6256138.1"
                     /translation="DAASQQTNTLVAPPVNFGASPYFLAYPGTLSLRLNTFVDLVEDL
                     VRSAYGQGFRRFLFLNGHGGNDSARVRLYELANQLPGMHLAWYAWWASHSVEVIAQKY
                     GLKPAHGNWLEGFTFTQVAELPDEGKIPPTVRGLLSAEEARHVYGDGSFGGPYKADKA
                     ILDEVFAACLSDILQLLQFE"
     gene            complement(546..1475)
                     /gene="miaA"
                     /locus_tag="AC16V7_03110"
     CDS             complement(546..1475)
                     /gene="miaA"
                     /locus_tag="AC16V7_03110"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIR6256139.1"
                     /translation="MSGEFKLPLVVILGPTAVGKTEIAIQLAERLEGEIVSADSRLFY
                     RGMDIGTAKPTPQQRKQVPHHLIDVANPDQPWSLAIFQQQAHLAISIIHSKRRLPFLV
                     GGTGQYIRAVIEGWQVPKAEPRPEMRQTLERWAAQITPLGLHHRLAILDPEAAARIEP
                     RNLRRTVRALEVILSTGRRFSEQRQRSSTPYRTIILGLMRSRTELYARVDERIQAMLA
                     SGLVEEVQTLLERGYSPNLPTLSAIGYREIISYLQGNTTLEEAVAQIKRRTRIFVRRQ
                     ANWFRSNDPNIVWFDVKPGIVDEMESIVQAWLG"
     gene            complement(1475..1843)
                     /locus_tag="AC16V7_03115"
     CDS             complement(1475..1843)
                     /locus_tag="AC16V7_03115"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR6256140.1"
                     /translation="MTSRLSLGRWGEAIAAEYLSSLGYVILEHNARTPYGELDLIAKQ
                     GDGLVFVEVKTRASLSLGLPEISVTPTKQAHLLSSAQAYLQAHPELGEDWRIDVIAIL
                     RLPGKSPQIEHFENIITSEA"
     gene            complement(1843..3033)
                     /locus_tag="AC16V7_03120"
     CDS             complement(1843..3033)
                     /locus_tag="AC16V7_03120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6256141.1"
                     /translation="MMADLYKPHVSGVTNYIALNKAYLEENGHEVFVFTFGDENIKDD
                     EANIIRSPGLPLLDTGFYINLSYNRDARKLLYSMDVVHVHHPFLSGSLALRYCRPRGI
                     PIVYTNHTRYDLYAQAYVPILGDLISETTMTTYLPVFCRLCDLVIAPSPGLRDVLMRF
                     GIDSPIAVVPNGVDLQPFRNPPRPMERSEFGFGPNDIVLIYVGRLGPEKNLIFLLRSF
                     AGAAKAYDNIALLLVGDGPERDNLEDRAQHMGIDDRVFFTGMIPYEKLPSYLSMADAF
                     VTASITEVHPLSVIEAMAAGLPVLGIKSPGIGDTVENGVTGLLTEEEELAMFTAKMVR
                     LVTEHQQRKEMGQRARQAAENYAIERTNMMMQEKYRFVYEKAGGRRSGWRVRFLRVLD
                     RWGS"
     gene            3355..4107
                     /locus_tag="AC16V7_03125"
     CDS             3355..4107
                     /locus_tag="AC16V7_03125"
                     /inference="COORDINATES: protein motif:HMM:NF040134.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5656 family protein"
                     /protein_id="HIR6256142.1"
                     /translation="MLAYALASFVDLPPREISVQLPGLYLAVELNARTVAALLVAGLT
                     ASGADWLLRTHPSVQGHKTFEHWLVPALTALVIGVPLSQISGAPQWWLGFIAGGALLM
                     LVLVAEYITVDSDDMRQPIAAAGLTTVSFALYLILAIALRYAGLRLFLLLPPLILAAW
                     LVSLRTLHLRLHGRWVFLQAGVVALICGQLAAVLHYLPLSPISFGLFLIGPAYALTSL
                     LANLAEDEPLRQAILEPLIVLAILLIITLWIG"
     gene            4116..>4450
                     /locus_tag="AC16V7_03130"
     CDS             4116..>4450
                     /locus_tag="AC16V7_03130"
                     /inference="COORDINATES: protein motif:HMM:NF025819.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HIR6256143.1"
                     /translation="MIEKLVMTREHWAQILTEVERVVPEEACGLLAGIDERVIESIPV
                     TNTLHSRVRFRMEPLEQLKAFQRIEAQGWDLIGIYHSHPLGQDGPSQTDIDEAYYPEV
                     VSVIVSGRDG"
BASE COUNT         1021 a         1084 c         1182 g         1163 t
ORIGIN      
        1 agatgctgcc agccagcaga ccaataccct ggtcgcaccg cctgtaaatt ttggagcctc
       61 accctatttt ctggcatacc ccggaacttt aagcctgcgc ttgaatactt ttgtcgatct
      121 agttgaagat ctggtgcgtt ctgcatacgg gcaaggtttt cggcgattcc tgttcctcaa
      181 tgggcacggc ggcaacgact ccgctcgcgt tcgtctctac gagctggcga accaattgcc
      241 tgggatgcac ctggcttggt atgcctggtg ggcttcacat agtgttgagg tgattgcaca
      301 gaaatatgga ctgaagccag ctcatggaaa ttggctggag gggtttacct ttacgcaggt
      361 ggcagagttg ccagacgaag ggaagatacc ccctactgtt cgcggattgt tgagtgccga
      421 ggaagcacga catgtatacg gagatggttc ttttggggga ccctataagg cagataaggc
      481 gattttggat gaggtattcg ctgcttgttt aagcgatata ttgcaattgt tacaattcga
      541 atgatctaac ccaaccacgc ttgtacaatt gattccatct cgtccacaat accaggtttg
      601 acatcaaacc agacaatatt cggatcgttt gatctgaacc agtttgcctg gcggcgcaca
      661 aatattcgtg tacggcgttt tatctgggca acagcttcct cgagggtggt attgccttgc
      721 aaataagaga taatttcccg gtaaccgatt gcagataagg ttggcaggtt gggagaatag
      781 ccacgttcca gtagggtttg aacctcttcc accagaccgg aagccagcat ggcttggata
      841 cgctcgtcaa cacgcgcgta taactcagta cgcgaccgca tcagtcccag tatgattgtc
      901 cggtatggag tggaggatcg ctggcgctgt tccgagaagc gcctaccggt actcaggatc
      961 acttctaggg cgcgcactgt gcgccttaga ttgcgcggtt caatgcgcgc tgctgcttca
     1021 ggatcaagaa ttgccagacg gtggtgaagg ccgagaggtg taatttgtgc cgcccaacgc
     1081 tccagtgttt gccgcatctc gggacgcggc tcagctttgg gcacctgcca gccttcaatg
     1141 acggcgcgga tatactgccc agtgccacct accagaaatg gtagccggcg ctttgaatga
     1201 attattgaaa tcgccaggtg cgcctgctgc tggaaaattg ccaggctcca aggttggtct
     1261 ggattggcca catcgatcag gtgatggggc acctgcttgc gttgttgtgg tgtgggtttt
     1321 gcggtgccaa tatccatccc ccgataaaac aaacgcgaat ccgctgatac aatctcaccc
     1381 tctaaccgtt cggcgagctg gatagcgatc tcggttttac ctactgcagt gggaccgagg
     1441 atgacgacca aagggagctt gaattcacct gacatcaggc ttcgctggtg atgatatttt
     1501 cgaaatgttc gatttggggg ctttttccgg gcaaacgcaa gatagcgatg acatcgatgc
     1561 gccaatcttc ccccaattca gggtgcgcct gcaggtatgc ctgggcagac gaaagcaggt
     1621 gagcttgctt ggtgggtgta actgagatct cgggtagccc gagcgataag gaggcgcgtg
     1681 tcttcacctc cacgaagacg agcccatcac cctgtttggc gatcaggtca agctcaccat
     1741 aaggggtgcg ggcattgtgt tcgaggatta cgtaccccag ggaagagaga tattccgctg
     1801 cgatcgcctc gccccagcgg cctaacgata ggcgggacgt cattagctgc cccagcgatc
     1861 cagaacccgc aggaatcgga ctcgccatcc actccttcgt cctcctgctt tttcatatac
     1921 aaaccgatat ttctcttgca tcatcatatt tgtccgttcg atggcgtaat tctcagctgc
     1981 ctgacgggct cgttgtccca tttctttgcg ctgctgatgt tccgtcacca accggaccat
     2041 ctttgcggtg aacatagcca gttcttcttc ctcggtcagc aaacctgtaa cgccattttc
     2101 aacagtgtcg ccgatgcctg gagatttgat gcccagcacg ggtaacccgg cagccatggc
     2161 ttctatcaca gaaagtgggt gtacttcggt tattgaagcg gtgacaaaag cgtctgccat
     2221 agaaagatag ctgggcagtt tctcataagg tatcattcct gtgaaaaaga cccgatcgtc
     2281 gatgcccata tgttgagcgc ggtcttccaa attgtctcgt tctggaccgt caccaacgag
     2341 caatagggcg atattgtcat atgctttagc agcaccggca aacgatctta gcaaaaatat
     2401 caggtttttt tccggtccaa ggcgacctac gtaaattaat acaatatcgt tggggccaaa
     2461 accgaattcg gaccgctcca tcgggcgagg aggatttcga aatggctgaa ggtctacccc
     2521 gtttggtact acagcgatag gcgagtcgat cccaaatcgc atcagcacgt cgcgcagacc
     2581 tggtgaggga gcgatgacca aatcgcatag acggcagaaa acgggaagat aggtcgtcat
     2641 ggtggtctcg ctgatcaagt cgcccaatat gggcacatat gcctgggcgt acaaatcata
     2701 acgggtatgg ttagtgtaaa caatcgggat gccacgcggg cggcaatagc gtaaggctag
     2761 ggaaccgctt aaaaagggat gatggacgtg tacgacatcc atggagtaga gcagttttct
     2821 ggcatcgcga ttgtagctca ggttgatata aaatcctgta tcgaggagag gtagccctgg
     2881 agagcggata atgttcgcct catcatcctt gatgttttca tcaccaaatg tgaaaacgaa
     2941 tacctcgtgt ccattctctt ctagataggc tttattcagt gcgatgtaat tagtgacacc
     3001 gctgacgtgt ggcttgtata gatcagccat cattccaatt cgcatagtcg accttttaac
     3061 gtccgtttct aatatgataa cctttgatca ttatactctg ggattgatag agcagagata
     3121 ttggcacggc agatcatcta tactgccaac tatctaaaat atagtcttgg gataagtaat
     3181 tgtggaaaga tgcgaattgc agggttgttg tattttcacg cgattctttt cctctttgaa
     3241 tgaggaagtc ctaaaaaatc accgcaattt tgcaggttgc ggtacaatcc agacgcagtg
     3301 ttacaacgcc atctccctga atctgaccgt cttagcatct tagcagccac gatattgctt
     3361 gcgtacgcac tggcgagttt tgtcgactta ccaccccgcg agatatctgt gcagttgccg
     3421 ggtttatatt tggctgttga gctcaacgct cgaaccgtgg cagccctact ggttgcgggc
     3481 ttaaccgcaa gtggagcgga ttggctctta cgaacacacc cttctgtgca agggcacaag
     3541 acgtttgagc attggctggt tccggctttg acagcattgg tgatcggggt gccactatcc
     3601 cagatctcag gcgcacccca gtggtggtta ggttttatcg ccggaggtgc tctgcttatg
     3661 ttggttttag tcgcagagta catcactgtc gactcagacg acatgcgcca gccaatcgca
     3721 gctgcaggat taaccacggt ctcctttgcg ttatacttga ttctggcaat cgcccttcgt
     3781 tatgcaggtt tgcgcctgtt tttactctta cctcctctca tcctggcagc ctggctggtt
     3841 agtttgcgga cattgcattt acggttacat ggccgctggg tatttttaca agcgggggtg
     3901 gtcgcgctga tctgtggaca gctagctgct gtgttgcatt accttcccct gtcgccaatt
     3961 tcttttgggt tatttttaat cggacccgcc tatgcattga ccagtctgct cgccaatctg
     4021 gctgaggatg aaccgctgcg ccaggcgatc ttagagcctc ttattgttct ggccattctt
     4081 ttgataatca ctttgtggat tggttagctt agcccatgat tgaaaaatta gtgatgacgc
     4141 gcgagcactg ggcgcaaatt ttaaccgagg tagagcgagt tgtgcctgaa gaagcttgcg
     4201 ggttattagc tggaattgat gaaagggtga tagagtccat accggtaacc aacacattac
     4261 atagccgagt gcgattccgc atggaaccct tggagcagtt gaaagctttt caaagaatcg
     4321 aagcacaggg gtgggatttg attggcatct atcactccca tccattggga caggatggac
     4381 cctcgcaaac cgatattgat gaagcttatt accccgaggt ggtttccgtg attgtgtcag
     4441 gacgagacgg
//
LOCUS       DBNHOH010000174         7278 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_651059, whole genome shotgun sequence.
ACCESSION   DBNHOH010000174 DBNHOH010000000
VERSION     DBNHOH010000174.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7278)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7278)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7278
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_651059"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="AC16V7_03135"
     CDS             <1..456
                     /locus_tag="AC16V7_03135"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013925290.1"
                     /note="catalyzes the formation of D-ribulose 5-phosphate
                     from 6-phospho-D-gluconate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HIR6256144.1"
                     /translation="SYTQGYMLMRTAAGEYEWNLNYGGIALMWRGGCIIRSAFLGKIK
                     EAYDRNPALPNLLLDPYFRREVSSSQAGWRRVLANAVQAGIPLPAISSALAFYDGYRQ
                     ERLPANLIQAQRDFFGAHTYERLDRPRGEFFHTNWTGEGGAVTSGSYQA"
     gene            complement(686..2248)
                     /locus_tag="AC16V7_03140"
     CDS             complement(686..2248)
                     /locus_tag="AC16V7_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR6256145.1"
                     /translation="MRSTQNSRWAFTSLDLRFGHFHQLISLALLLLLGLVLIQSISLG
                     RITNFPSRPANPVPVWGEKPVLGYFVFTPSLASRLSREAGLSEAQFQAIEQITHQELE
                     VLRAIEQESLPIIQDSELSLEEKRSLIVKMRYNERVRGVVRASRDKLKNTLGIFAYAR
                     LSQWIQRQWQNEREIHGSVETASGPRTYRIFATRYDAGGAYTVALPDKCLKFANGGNH
                     ICDSDGYVAGAGYTVFLSYEGSTAAIVGESGPWNVDDNYWATTSDPTPRRMFADLALG
                     MPEAQAAYFNGYNGGVDQFGRVVTAPFGIDLARQVSIDIGLQPGNNDWIDVSFLWTEG
                     WDGSAGSQPPQSDATAIPQPTKETIIPIEVASPNPDGSVVHVVQEGQTLWNIASAYQV
                     SLQEILVLNGLSDGALIYPGQKLLIKLASQTPTLTSTSTTETEIPSSVSTATLRPTRT
                     VVPRSATAIAQIVSTPLPPQNTPNAVPTAGVSSSDPDAEANVDPFIIGIVALAGVGLF
                     LLILGQWLNRRS"
     gene            complement(2397..3239)
                     /locus_tag="AC16V7_03145"
     CDS             complement(2397..3239)
                     /locus_tag="AC16V7_03145"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008183115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR6256146.1"
                     /translation="MKYRRIIFTLLLIIVSVSLVAAAGAKKQDTLWDIWKILQKKEFV
                     DLTHTFEPGIPHWPGFPDEERELFFWYDDQPPGPGTLGTGFYAQEFCHVGQWGTHVDP
                     PAHFIRGLRTIDQIEVKEMVLPLVVIDVHEKVEDNPDYTITMDDVRRWEKRHGKIPKG
                     AFVAMRTDWSKRWPDMAAMQNKDADGIAHYPGWSQEVLTYLYEVRKITASGHEPTDTD
                     PGIATSQGDYSLEAYILNTDHYQIELLNNLDMVPEAGALVIVAFPKPKDGSGFPARVF
                     AINP"
     gene            3776..4024
                     /locus_tag="AC16V7_03150"
     CDS             3776..4024
                     /locus_tag="AC16V7_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR6256147.1"
                     /translation="MPVKGWIVVDDLYCKGCELCVDACPQDVMGIDMTRLTPKGYHPA
                     ILKAEGCTGCAICAVVCPEAALTVYRETPVRAARAAAA"
     gene            4033..5166
                     /gene="vorB"
                     /locus_tag="AC16V7_03155"
     CDS             4033..5166
                     /gene="vorB"
                     /locus_tag="AC16V7_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HIR6256148.1"
                     /translation="MPRELLKGNNAMAEAAIRAGVEAYFGYPITPQTELLEHFSQRMP
                     ELGRVFLQAESEVAAINMVYGAACTGARVMSSSSSPGVSLMLEGISYIAGTEIPVVLI
                     DVVRGGPGLGNIAPSQGDYNQIVHGGGHGDYHPIVLAPASVQEAIDLTVLAFDLADQY
                     RTIVIVLADGNIGQMMEPAELPPMRPLRKEYPDWAVRGASGRERRILTSIYIDPKDEE
                     VVNYRLMKVWNQIQANEVRYKEYYLDDAEFVVVGYGTAGRVALSAVREARSQGIKAGL
                     FRPVSLSPYPYAQLEALTDRTQAFLVTEMNGGQMLDDVLKAIKGRVPVEFYGRMGGMV
                     PFPDEILAEIQRIAQEPLSIKENPRLDWMKRIAGIIDAARSRN"
     gene            5179..5955
                     /locus_tag="AC16V7_03160"
     CDS             5179..5955
                     /locus_tag="AC16V7_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR6256149.1"
                     /translation="MELSAQAQIVYQRPESLADVPSHYCPGCTHGIAHRLLAEVLDEM
                     GVRENTIGVAPVGCSVFAYNYFDFDFVEAAHGRAPAMATGIKRLLPERVVFTYQGDGD
                     LASIGMAEIMHAAGRGENLTVVFINNAIYGMTGGQLAPTTLPGQKTTSSPSGRDVEVM
                     GFPLRMSEVLSTLDGAGYIVRRSLHDPKNIRMAKKAIRLAFETQKRGLGFSMVELLST
                     CPTNWGITPLEALRWLEEHMIPVYPIQDFKVSSTIAGLRI"
     gene            5969..6535
                     /locus_tag="AC16V7_03165"
     CDS             5969..6535
                     /locus_tag="AC16V7_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HIR6256150.1"
                     /translation="MQMEIVIAGFGGQGVLFAGQVLAYAAMDNGKEVTWIPSYGPEMR
                     GGTANCTVIIADEEIGSPLVLNPKAAIVLNLPSLDKYESLIQPGGVLVANASLINRGP
                     SRSDIKSVFIPANEIAESIGNKRLTNMIALGALLGAMPILSLDAIDQALKDHLPDRHR
                     KMLPENFKALRQGAEFTQKTLADVIAPS"
     gene            6776..>7278
                     /locus_tag="AC16V7_03170"
     CDS             6776..>7278
                     /locus_tag="AC16V7_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256151.1"
                     /translation="MNNKIMQLISLLLVLSFLVSCTAATPVPPTPTPEPPTVTQLPPT
                     ETPLPTDTPTFTPLPPTDTPTQEPTFTPIPTETETPTSTASFTPSPPPLTHFIPLPAA
                     SNNTVNIYFIELNAGNAACGDRILAVSSGVEKSGNIQKDVAAGLRKLFSYKEKFYGDL
                     YNPLYAS"
BASE COUNT         1720 a         1785 c         1952 g         1821 t
ORIGIN      
        1 tcctacacgc agggatatat gcttatgcgc acggccgccg gcgagtatga atggaacctc
       61 aactatggag ggattgcctt gatgtggcgc ggtgggtgta ttattcgctc cgccttcctg
      121 gggaaaataa aagaagctta tgatcgtaat cctgccctac ccaacctgtt gttggaccca
      181 tatttccgcc gcgaagtgtc gtcttcccag gctggttggc gcagggtgct agcgaacgca
      241 gtacaggcgg ggatcccact cccagcaatt tccagcgcac tggcgtttta tgatggctac
      301 cgtcaggaac gcttaccagc gaatttaatc caggctcagc gggacttctt cggggcgcat
      361 acctacgagc gcctcgatcg gccgcggggc gaattcttcc ataccaactg gaccggcgag
      421 ggtggggcag tcacttcggg gagctatcag gcctaaatag cagtatccat tgcatcggga
      481 cctggcccga gttgaacaga ataatccaag ttgccataat agccatgccc atcatagtct
      541 tttataggga ggaatattgt atgtcggcgg gtcacggatt acagcgatat ccccccaatt
      601 ccccgtattg cggtaatgca tacacctggc agggcaatta ctttgcacta ccaaaatgat
      661 ggtcacatgg taatttgaaa aattattatg atctgcggtt caaccactgg cctaagataa
      721 gcagaaataa acctacgcct gcaagtgcta caatcccgat gatgaatggg tccacatttg
      781 cttcagcgtc tgggtcagaa gaggagactc ctgccgtggg gactgcgttt ggggtgttct
      841 ggggtggcaa aggtgtagag acgatttggg cgatggcggt agcagaccta gggaccactg
      901 tccgagtcgg tcgcagggtg gcggtcgaga cactggaggg tatttccgtt tcggttgtac
      961 tggtggaagt caaggtgggt gtttggctag cgagtttgat cagcagtttt tgaccaggat
     1021 agatcagtgc cccgtcgctt aatccgttca acactaggat ctcttgaaga ctgacttgat
     1081 acgctgaggc gatattccaa agcgtttgtc cctcttgcac cacgtggacc actgagccat
     1141 ctgggttggg tgacgcgacc tcgatgggaa taatggtttc ttttgtgggc tgcggtattg
     1201 cggttgcatc ggattgggga ggttgtgagc ccgcgctgcc atcccagccc tcagtccaca
     1261 agaaagaaac atcaatccag tcgttattgc ctggctggag accgatatcg atactcacct
     1321 ggcgtgccag gtcgatgcca aagggtgcgg ttaccacccg cccaaattga tccacaccac
     1381 cgttataccc attaaaataa gctgcttgtg cttctggcat gcccaatgct aggtcggcga
     1441 acattcgccg gggcgttggg tctgaggtgg ttgcccagta attatcatcc acgttccaag
     1501 ggccagattc tccaacgatt gctgccgtac tgccctcata gctgagaaat actgtatagc
     1561 cggcaccggc aacatagcca tcgctgtcgc agatgtggtt tccgccgttg gcgaacttta
     1621 ggcacttatc cggaagggcg acagtatatg ctccgcctgc gtcataacgg gtcgcaaaaa
     1681 ttcggtaggt gcgcggtccc gaggccgtct ccacagagcc gtgaatctcc ctctcattct
     1741 gccattggcg ctggatccat tgagagagcc gtgcgtaagc gaatatcccc aacgtgtttt
     1801 taagtttatc cctggatgcc ctgacaactc cccggacgcg ttcgttatat ctcatcttga
     1861 cgatgagcga acgtttctct tccaggctta gctcggaatc ctggattata ggtaggctct
     1921 cctgttcgat ggcgcgcaac acctctagtt cttggtgtgt gatttgctcg atggcttgga
     1981 actgagcctc ggatagccct gcttccctgg ataatcggct tgccaggctg ggtgtgaaga
     2041 cgaagtaacc aaggacaggc ttttcacccc aaacgggtac gggattagcc gggcgagatg
     2101 ggaaatttgt gatacgccca agggagatcg actggatgag cactagcccc aatagcaaaa
     2161 gcaaagctag gctgattagc tgatgaaaat gcccgaatct caggtcaagg gatgtaaaag
     2221 cccatcgact gttttgcgtt gatctcataa gtttgagcga attataatgg tctttttagt
     2281 gatgagcttg gagaagccac gcgtaaccaa gaggggatat gcaagcatca agtgttgcat
     2341 atccccacct taggaagtta acctaaaact atgggcagga ccgggaatgt tttattctat
     2401 gggttgatcg caaatacgcg ggccgggaaa cctgaaccat ctttgggttt tgggaaggct
     2461 acaatgacca atgcgccagc ttcaggaacc atgtccaagt tgttcaatag ctcgatttgg
     2521 tagtgatcgg tgttgagaat ataggcttcc aacgagtaat caccctggct ggtggcgata
     2581 ccggggtcgg tatcggttgg ctcgtgtccg gaagccgtga tctttcgcac ctcatacagg
     2641 tatgtcagca cctcctggct ccagcctggg tagtgggcga tgccatccgc atctttgttt
     2701 tgcatagctg ccatatcagg ccaacgcttc gaccaatccg tgcgcatggc aacaaaagcc
     2761 cctttgggga tcttaccatg cctcttttcc catcgacgca cgtcatccat tgtaatggtg
     2821 taatctggat tgtcttccac tttttcatgc acatcgatca ccaccaatgg caggaccatc
     2881 tcctttactt cgatctggtc gatggtgcgc aggccacgga tgaagtgggc tggtggatcg
     2941 acatgggtgc cccattggcc cacatggcag aattcttggg cgtagaagcc tgtgcctaga
     3001 gtgcccggcc cagggggttg gtcatcgtac cagaaaaata gctcgcgctc ctcatcgggg
     3061 aagcccggcc agtgtggaat ccctggttca aaagtatggg ttagatcgac gaattctttc
     3121 ttttgaagta ttttccaaat atcccagaga gtgtcttgtt tttttgctcc agcagctgcc
     3181 acaagggaca ctgaaacaat gattagtaat aaggtgaaaa tgatacgcct gtatttcatt
     3241 ttatcctcca tcagttatcg aattgaaata cggatcaggc ggaatattac ggcaatgata
     3301 tgtgatgtgt ctcctggtcc cacccatttt gccaactcta taagatagca tagcaaattt
     3361 tagaagaaat ctcaagtatt tttagaattc cttaaaacga gcgctcggtt ttgccaaata
     3421 gctaccgagc gcaagtttca ttatgtagat ttgtactgga tttaatagat tatctcgaac
     3481 ctgtatgtta cagcagtagt attgaaatcg aaattcaggt tatatatttt atagtataaa
     3541 aacgacttcg tgtactttat tgggaaaata tccctatttg ggcaaagtgt caattggcat
     3601 aaccagttaa tgacagttag cactattcaa aggcattgta gaataagata atataggttg
     3661 gaattgcatc gataggagtt gcacagttga tggaatttgt ggaaaaacga atctccaaat
     3721 tccgcccaac tgggtatttc tggtttaata aacgatatcg accaggaggt tgttaatgcc
     3781 tgttaagggc tggatcgtcg tggacgatct gtattgcaag ggatgcgagc tttgcgttga
     3841 tgcctgcccg caggatgtga tgggtatcga catgacccga ttaacaccca agggctacca
     3901 tcctgccatc ctcaaagctg agggctgtac agggtgcgcg atttgcgcgg tagtatgtcc
     3961 cgaggcagcg ctgacggttt atcgcgagac ccctgtgcgt gctgcgagag cggcggcagc
     4021 ttaggaggac gtatgcctcg agaattgctt aagggaaaca atgctatggc cgaagcggca
     4081 atccgcgctg gcgttgaggc gtatttcggc tacccaatca cacctcaaac cgagttgctg
     4141 gaacatttct cccagcgtat gcccgagctt gggcgtgtct tcctgcaggc agagagtgaa
     4201 gttgctgcca tcaacatggt gtacggagca gcctgcaccg gggcgagagt gatgagttca
     4261 tcctccagcc ccggtgtaag cttaatgttg gaaggtattt cctacattgc agggacagag
     4321 ataccagtgg tgttaattga tgtggtgcgt ggcgggcctg gtctgggaaa cattgccccc
     4381 tctcaggggg attacaacca aatagtgcat ggaggcgggc atggcgatta tcaccccatc
     4441 gtgttagcgc ctgccagtgt gcaagaggca attgatctga cggtgcttgc cttcgacttg
     4501 gcagatcaat atcgcacgat tgtgatcgtc ttagcggatg gaaatatcgg ccagatgatg
     4561 gagccggcag agctacctcc catgcgtcca ttgcgcaagg aatatcccga ttgggcggta
     4621 cgcggtgcct caggccgcga gcggcgcatt ctcacctcga tttacatcga tccgaaagat
     4681 gaagaggtgg tgaactatcg cctgatgaaa gtctggaatc aaatccaggc caatgaggtg
     4741 cgctacaagg agtattatct ggatgacgcg gagtttgtcg ttgtgggtta cggcaccgca
     4801 gggcgcgttg ccctgtctgc agttagagag gcgcgcagtc aagggatcaa ggcaggctta
     4861 ttccggccag tctcactcag cccctatcca tatgcccaac tggaggcctt gacagaccgc
     4921 acccaagctt tcttggtgac cgaaatgaac ggcggtcaga tgctggatga tgtcctcaag
     4981 gcgatcaagg gtcgcgtgcc agtagagttt tacgggcgca tgggcggtat ggtcccgttc
     5041 ccggatgaga ttctggcgga aattcaacgc atcgcacagg agccgctatc tatcaaagaa
     5101 aatccgcgcc tagattggat gaagcgtatt gctggaatca tcgacgcggc taggtcaagg
     5161 aactgaggag ctctgctaat ggaattgtca gctcaggctc aaattgtcta tcaacgcccc
     5221 gaatctttag ctgatgtgcc atcccattat tgtcctgggt gtactcatgg gattgcacac
     5281 cgcctacttg cagaagtgct ggatgagatg ggggtgcgtg aaaacacgat tggtgtagcg
     5341 ccggtaggct gctctgtttt tgcctacaat tattttgatt ttgactttgt ggaggcagcg
     5401 cacgggcgcg ctccggccat ggcaacgggc atcaagcgcc tgctccccga acgagtggtt
     5461 tttacatatc agggggatgg cgacctggcg tctattggca tggcagagat tatgcacgct
     5521 gcaggtcgcg gggagaatct caccgttgtt tttatcaaca acgccattta cgggatgaca
     5581 ggggggcagt tagctcccac cacccttcca ggccagaaga cgacctcctc ccctagcggg
     5641 cgcgatgtgg aggtgatggg cttccctcta cgcatgtcgg aggtattatc gacactggac
     5701 ggagccggtt acattgtgcg gcgaagtctg catgatccga agaatatccg catggctaaa
     5761 aaagccatcc gtttagcctt tgaaacgcag aagcgtgggc tggggttctc aatggttgaa
     5821 ttgctctcta cctgtccgac gaactggggt attactccct tagaagcatt gcgctggctg
     5881 gaagagcaca tgatacccgt ctatcccatc caggacttca aagtgtcctc tactatagcc
     5941 gggctgcgta tttagggaga tgatcacgat gcaaatggaa attgtcatcg caggttttgg
     6001 tgggcaggga gtactgttcg cagggcaggt tttagcctat gccgctatgg ataacggaaa
     6061 agaggtgacc tggattcctt cttatgggcc agaaatgcgt gggggcaccg caaactgcac
     6121 agtcattatc gctgatgaag agatagggtc gcctctggtg ctcaatccca aggcagccat
     6181 tgtgctcaac ctgccctcgc tggataaata tgaaagcctc atccaaccgg ggggtgtctt
     6241 ggtagccaac gcctcattga tcaatcgtgg tccatctcgg agtgatatca aatcggtttt
     6301 catcccagcc aacgagattg ccgaatcgat tggcaacaaa cgcctgacca atatgattgc
     6361 actcggggct ctgctgggcg cgatgccgat tctctcattg gatgccatcg atcaggcgct
     6421 caaagaccat ttaccagaca ggcatcgcaa aatgttgcct gagaatttca aggcactgcg
     6481 ccagggggca gagtttacgc agaaaacgct agccgatgtg attgcaccct cgtaaggatt
     6541 agattggcat agtggagtct gtaatggcag gaaggcgcta caacagaccc taattctgcc
     6601 ttttttgctt catttacccc ctttcctaat gtgtcaagat ggcttttttg tgctatcacg
     6661 atttggtgat agaattgaaa acttgaggat tttggctttt tcgcgttttt accaagaccc
     6721 tacctgtatc ctttttctcg tctcagttaa accgcaagat tagtcttgag ggcttatgaa
     6781 taacaagatc atgcaattaa tctcattgct cttagtttta tctttccttg tgtcatgcac
     6841 ggcagcaacg ccagtaccgc ccacccccac gcctgaaccg ccgacagtta cacagctgcc
     6901 tccgacagag accccactgc ctaccgatac acccacattt accccgcttc cgccgacaga
     6961 tacccccacc caagagccta cttttacacc aatcccgacg gaaacggaaa cacccacttc
     7021 aacggcatct ttcacgcctt cacccccacc attgacacac tttatcccct tgcctgcagc
     7081 cagtaataac acagtgaata tctacttcat tgagcttaac gcggggaacg ccgcatgtgg
     7141 agaccggata ctcgcagtta gctcaggcgt ggagaaaagt ggaaatatcc aaaaagatgt
     7201 ggcagccggt ttgagaaagc tctttagtta taaggagaag ttctatggcg atttgtacaa
     7261 cccgctgtac gcttctag
//
LOCUS       DBNHOH010000175         4316 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_586982, whole genome shotgun sequence.
ACCESSION   DBNHOH010000175 DBNHOH010000000
VERSION     DBNHOH010000175.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_586982"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            344..982
                     /locus_tag="AC16V7_03175"
     CDS             344..982
                     /locus_tag="AC16V7_03175"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HIR6256152.1"
                     /translation="MESDAGTALRPLKKVPFVVSLVIVLLMAVAYTVLDTLQPHEPTL
                     SILAFIPGTISILALLSAGFDRHECYLRFARLSRAGALALALTTVLLLPILSSSTAWG
                     GWDWLPALVYAPASGLAQELYFRSGLLPALEKAFQGNKKSALFAHAVVFVGFHYRTFQ
                     AIPSVGPGIIVAAVLFLAGCGWGWQVQRDSTVVWAVVQHSFFLVLMSMFDWA"
     gene            1037..2527
                     /locus_tag="AC16V7_03180"
     CDS             1037..2527
                     /locus_tag="AC16V7_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR6256153.1"
                     /translation="MKSQFNFRLLSLALIPALLLSSCAPQIVSTSSGAAAEAAPPLQP
                     TPEASAPTPMPQRPVYAPGELVDYTAQTGDTLPSLAVRFNTSVDEIRQANPIIPESAT
                     SMPPGMPMKIPIYYLPFWGSSYQILPDSLFVNGPAQVGFDTAKFIAGQPGWLKGYTEY
                     AADENRSAANMIDLVVLRYSVSPRLLLALLEYQSGALSQAEPSEEAQTYPMGYDRRER
                     RGLYRQLIWAANLLNNGYYGYRTNHLTSLDYEDGRMERFDPWQNAATVGLMNFFNTLL
                     PKDAYLQAISSDGFAATYASLFGDPWAADQPHIPGSLEQPEFALPFKAGEIWAFTGGP
                     HTAWGIGDPLSALDFAPPSVAGGCQETDLWATAVADGVIARSETGEIALDLDRDGDER
                     TGWVVFYLHLATQGRAPLGAEVVAGQPVGHPSCEGGSSTGTHVHIARKYNGEWMPAEG
                     TLAFNLEGWQARNGSQPYQGTLTRLSRTVTACVCSNRDTFIQSEKR"
     gene            2534..3847
                     /locus_tag="AC16V7_03185"
     CDS             2534..3847
                     /locus_tag="AC16V7_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6256154.1"
                     /translation="MPVERPTGMFGFTIVWIGQFVSLLGTSMTAFSLTIWAFELTGSA
                     TALALVGFFYVTPMLIFSPFAGAIVDRANRKWMMALSDLASGMATLGILGLYLSGQLQ
                     IWHLYVANVIIGLFQSFQWPAYSAAISVMLPKEHYGRANGMMSLAESGSGIFAPLLAG
                     AVLGIIGLGGVLFIDVATFLFAIATLTVIHVPQPTVTEDGLQGRGSLLQEAVFGFRYI
                     LARPSLLGLQSVFLLGNFFSSAMFIMLAPMILARTSNNELIYGSVMSAGAVGGVIGGL
                     AMSAWGGPKRRIHGVLGGWIVASILDGVVMGLGRDLPVWAAASLLGAFFIPVINGSNQ
                     AIWQAKVAPDVQGRVFSIRMLIAWFVNPVAMLVVGPLADYVMEPAMQAGGTLSGTFGW
                     LVGTGPGAGIALIFIFSGLMASLISMGAYTVTAIRDVESILPDHS"
     gene            complement(3849..>4316)
                     /locus_tag="AC16V7_03190"
     CDS             complement(3849..>4316)
                     /locus_tag="AC16V7_03190"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR6256155.1"
                     /translation="EAKVIWQGGMKLDGLADSGLHLPLGSRPAGDGDDQGFLPMELLA
                     TGLAGCTAMDVISILQKKGQDVTGFEVQVHIQRAPEHPRVFTHALIEYFVTGRRVDEA
                     AVVRSIELSATRFCPALAMFKKVFSIEMKYHVFEAGDDDKRQLVKSGEYAYPV"
BASE COUNT          789 a         1285 c         1229 g         1013 t
ORIGIN      
        1 tcattaactc ttgattagct caaagaattt gatagagagt gttaaaaaag ccatatatgg
       61 gggttgtttt ttgggattct ggcaaaaata tccccacctg tgtgggatag ccagagtttt
      121 acaacaccct cctggtgatt accaggttct tcaacaaagg agctggtgaa tttcggaaat
      181 tcgcagacag aataggtgag tatcttcgcc caggctacca agccttccta gatctcgcca
      241 gcatccaggc cgggcaaata ttcgaggcgc aattggggtt atactaatat cggtcgaggc
      301 ggtatggtta gatttcttgc cgcgtttggg agagggagga attgtggaaa gtgatgcggg
      361 gaccgcttta cgccccctga aaaaagttcc ttttgtggtc tctctggtga tcgtcttgct
      421 catggcggtt gcctatactg tcctcgatac cctccagccg cacgagccga cgctatccat
      481 tctcgcattc atccccggga cgataagcat cctggcgctt ctctcagcgg gtttcgaccg
      541 gcatgagtgc tacctgcgct ttgcccgtct ttcgcgggcg ggggcgctag ccctggcttt
      601 gaccaccgtg ctgctcctgc ctatcttgtc ctcgagcacg gcatggggcg gctgggactg
      661 gcttcccgcc ctggtttatg cgcccgcttc cggcctggcg caagagctct actttcgatc
      721 tgggctcctg cctgcccttg agaaggcatt tcaagggaat aagaaatccg ccttattcgc
      781 ccatgccgtg gttttcgtag gcttccatta ccgcacgttt caggccatac cttccgtggg
      841 cccggggata atcgtggctg ccgtcctgtt cctggcgggt tgtggctggg gatggcaggt
      901 tcagcgagat agtacggtcg tgtgggctgt tgtgcagcac agttttttcc tggtgttgat
      961 gtcgatgttt gattgggctt gagcatctct gcgctatact ctattgccga ataggtgtgg
     1021 gttataattc cctcccgtga aaagccagtt taattttcgt cttctatcac tcgctctcat
     1081 acccgccttg ctgctctcct cttgcgcccc gcaaatcgtc tccacctcct ctggcgcggc
     1141 ggcagaggca gctccaccgt tgcagccaac ccctgaagcg tctgcaccca cccctatgcc
     1201 acaacgcccg gtctatgcgc ccggcgagct ggtcgattac actgcgcaaa ccggcgatac
     1261 cttgccttcc ctggctgtgc gcttcaatac cagcgtggat gaaattcgcc aggccaaccc
     1321 gatcatccct gaatcggcga ccagcatgcc gcccggcatg cccatgaaga tcccaatcta
     1381 ctacctgccc ttctggggca gctcctatca aatcttgccc gacagcctgt tcgtcaatgg
     1441 cccggcgcag gtgggtttcg acaccgccaa gttcatcgct ggacagcccg gctggctcaa
     1501 gggatacacc gaatatgccg ccgatgagaa ccgctccgct gccaacatga tcgatctggt
     1561 ggtcctcaga tatagcgtca gcccgcgcct gttattggca ttgctcgagt accagtccgg
     1621 cgcgctcagc caggccgaac cctccgagga ggcgcagacc tacccgatgg gttacgatcg
     1681 tcgcgagcgc agaggcctgt accgccagct catctgggct gccaacctgc tcaacaacgg
     1741 ctactacggg tatcgcacca accatctgac cagcctcgat tacgaagacg ggcgcatgga
     1801 gcgcttcgac ccctggcaga acgcggcgac cgttggcctg atgaacttct ttaacactct
     1861 gttgcctaaa gacgcctacc tccaggctat ctcgtccgat ggtttcgccg ccacgtatgc
     1921 gtctctcttc ggcgacccct gggcagccga ccagccgcac atccctggca gcctggaaca
     1981 acctgaattt gcattaccct tcaaagctgg tgaaatctgg gccttcactg gcggaccgca
     2041 cactgcctgg ggcatcggcg accccctgtc tgctctggac ttcgccccac cgtctgtggc
     2101 gggcggctgc caggagacag acctgtgggc gactgctgtg gcggatggcg tgatcgctcg
     2161 ctctgaaacg ggcgagatcg ccctggacct ggaccgagat ggcgacgagc gcaccggctg
     2221 ggttgtcttc tacctgcacc tggcgacaca agggcgcgct cccctggggg ccgaggtagt
     2281 agccgggcag cctgtgggac atccttcctg tgaagggggc agcagcacgg gcactcacgt
     2341 gcatattgcc cgcaagtaca acggcgagtg gatgcctgcc gaaggaacgc tggctttcaa
     2401 tctggaggga tggcaggcgc gcaacggcag ccagccttat caaggcacgc tcacccgcct
     2461 gtcgcgcaca gtcactgcct gtgtatgctc taaccgagat accttcatcc agtccgaaaa
     2521 gcgctaaaac accatgccag tagaaagacc caccggaatg tttggcttca cgatcgtctg
     2581 gatcgggcag tttgtgtcgt tgctgggcac cagcatgact gccttttctc taactatttg
     2641 ggcctttgag ctcaccggct ccgccacggc tctggcgctg gttggtttct tctacgtcac
     2701 ccccatgttg atctttagcc cgtttgccgg cgcgatcgtc gatcgcgcta atcgcaagtg
     2761 gatgatggcg ctcagcgacc tggcttcggg gatggctaca ctgggtatcc ttggcctgta
     2821 tttgtctgga cagctacaga tctggcactt gtatgtagcc aacgtcatca taggcctctt
     2881 ccagtccttc caatggccgg cttattcagc tgccatcagt gtcatgttgc ccaaggagca
     2941 ctatgggcgc gccaatggaa tgatgtcgct ggccgagtcc ggttcgggta tattcgcccc
     3001 cctgctggct ggggcagtgc tgggaattat cgggctgggc ggcgtgctat tcatcgatgt
     3061 ggccaccttc ctattcgcca tagctacctt aacggtgatc cacgtgcccc aacccacagt
     3121 gacagaagac ggtttgcagg ggcgcggcag tctgctacag gaagctgttt tcgggttccg
     3181 ttatatcctg gctcgcccca gcttattggg gctgcaatcg gtgtttctat tggggaactt
     3241 cttctccagt gctatgttca tcatgctggc tcccatgatt ctggcgcgca ccagcaataa
     3301 cgagctaatc tatggcagtg ttatgtcagc gggagcggtg ggcggggtga ttggagggtt
     3361 ggcgatgagc gcctggggcg ggcccaagcg ccgcattcac ggcgtgctgg gtggttggat
     3421 tgtggccagt atattagatg gtgtggtgat gggtttaggg cgcgacctgc ccgtatgggc
     3481 tgccgcaagc ctgctggggg ctttctttat ccccgtcatc aacggctcca accaggctat
     3541 ctggcaagcc aaggtggctc ccgatgtgca gggacgggtg ttctccatcc gcatgctgat
     3601 tgcctggttc gtcaaccctg tggcgatgct ggtggtcgga ccgctggcgg attatgtcat
     3661 ggaacccgct atgcaggcgg gcggtacgct cagcggcaca ttcggctggc tggtaggcac
     3721 ggggcctggg gcgggcatcg ccctgatctt tatcttctca ggcctgatgg ccagcctgat
     3781 aagcatgggt gcctacaccg ttaccgctat ccgagatgta gagagcatct tgccagatca
     3841 tagctaagtt aaactgggta tgcatattcc ccgctcttga ccagctggcg cttgtcatca
     3901 tcccccgcct cgaagacatg gtatttcatc tcgattgaga acactttctt gaacatggct
     3961 agtgccgggc agaagcgcgt ggctgaaagc tcgattgagc gcacaactgc cgcctcatcc
     4021 acccgtcgcc cggtgacgaa atactcaatc agcgcgtggg taaatacccg ggggtgctcc
     4081 ggcgcgcgct ggatgtggac ttgcacctca aaaccggtca catcctggcc ctttttctgc
     4141 aagatggaga tcacatccat cgctgtgcag cccgctaagc cggtggcgag cagctccatt
     4201 ggcaggaacc cctgatcatc accgtccccg gcgggcctgg accccaacgg caggtgtagg
     4261 ccgctgtctg ccaagccatc cagtttcata cctccctgcc agatcacttt tgcttc
//
LOCUS       DBNHOH010000176         2498 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_523367, whole genome shotgun sequence.
ACCESSION   DBNHOH010000176 DBNHOH010000000
VERSION     DBNHOH010000176.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_523367"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="AC16V7_03195"
     CDS             <1..811
                     /locus_tag="AC16V7_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6256156.1"
                     /translation="IDTIYHLAALLSTRSEFTPEAAHRVNVEGTLVLLRLATEQSAWR
                     GTPVKFIFPSSIAAYGMPDLATKKAYARVREWEWNYPRTMYGCNKLYCEQLGIYFSRH
                     YRQLAADRSVMLDFRSLRFPGLISAFTAPSGGTSDYGPEMLHAAAKKVPYACFVREDV
                     QIPFMAMPDAVTALLRLTESPREALSRLVFNVTSFSLTAAEFREWVLKYFPDAQISFY
                     PDVKRQGIVDSWPADLDDSAARRDWGWQPDYDLERSFSEYLVPNIMKRYSA"
     gene            883..2049
                     /locus_tag="AC16V7_03200"
     CDS             883..2049
                     /locus_tag="AC16V7_03200"
                     /inference="COORDINATES: protein motif:HMM:NF049034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysS/YqeB C-terminal domain-containing protein"
                     /protein_id="HIR6256157.1"
                     /translation="MDITDYPEVKPGPIVLFGSGETAPSGRKIFDHVMRQLPPKPKLA
                     LLETPAGFELNSSQVIGRVGEFISHRLQNYDPQITIVPARKRGTAFSPDDPDLIEPLY
                     EADMIFMGPGSPTYAVRQLRGSLAWHALLARHRLGATIVFASAATVAASLFSIPVYEI
                     YKVGEELHWKDGLDFFGLYGLPLVFVPHWNNNDGGEELDTSRCFMGKPRFAELMEMIP
                     SELSILGIDEKTALVMDPQSCACRVIGLGGVTIIHAGPAHRSATQPSQLDGTGLNEVA
                     ENRKAHVHHYQHGQAFSLKRIGPFCDPEAGVGLPGEVWRRALEVHHARQSQEMASFED
                     ESPPPEVLVLVEERQAARARKDWAASDSLREQVADLGWLIQDTPEGPKVVKSDD"
     gene            2083..>2498
                     /locus_tag="AC16V7_03205"
     CDS             2083..>2498
                     /locus_tag="AC16V7_03205"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6256158.1"
                     /translation="MTQTLSVGYIGLGLMGKSIARNILKAGFPLVVHNRSQAAVEELV
                     AEGAVRAGSAAEVASSVDVVFTNLPDTPDVEKVALGSNGVIEGAHPGLVFVDNSTIQP
                     AAARRIAEVLGEKGVQCLDAPVSGGDIGARQGTLAI"
BASE COUNT          510 a          655 c          754 g          579 t
ORIGIN      
        1 gatcgacacg atttaccacc ttgcagcgtt gctttccacc cgctctgagt ttacccccga
       61 ggctgcccac cgtgttaacg tggagggtac gctggtgcta ttgaggctgg caaccgagca
      121 gtccgcatgg cgaggcacgc cggtgaaatt catttttcca agctctattg ctgcctatgg
      181 catgccggat ctggcgacca aaaaagccta cgcccgggtg cgcgagtggg agtggaacta
      241 cccccggacg atgtatggct gcaacaagct ctactgcgag caattgggca tttatttcag
      301 ccggcactac cgccagctgg cagctgaccg atctgtgatg ctggattttc gcagcttgcg
      361 ttttcctgga ttgatcagcg ccttcactgc gcccagcggt ggcaccagtg attatggtcc
      421 cgagatgctc cacgcggctg ccaaaaaggt tccctatgcc tgctttgtgc gcgaagatgt
      481 gcagatcccg ttcatggcaa tgccggatgc ggtgacagcg ctccttcgcc ttaccgagtc
      541 tccgcgtgag gctttgagcc gcttggtgtt taatgtgacc agcttcagcc tgaccgccgc
      601 cgagtttcgc gagtgggtat tgaagtattt ccctgacgcc cagatttcgt tttatcccga
      661 tgtgaagcgt cagggcatcg tggatagctg gcctgctgac ctggatgaca gtgccgcccg
      721 ccgagattgg ggttggcaac ctgattatga cctggagcgc tctttcagcg agtatctggt
      781 accaaatatc atgaagcgtt acagcgcttg atctcactat cgagcaacca atcgtgcaca
      841 gggtagaata tttaagcgaa tttattgatt agagatagag atatggatat aacagattac
      901 cctgaagtaa aacctggccc gatcgtactc ttcggctcgg gcgagaccgc tcccagcggg
      961 cgtaaaatat tcgatcatgt catgcgccag ttgccgccca aaccaaaact ggctttactg
     1021 gagaccccgg cagggtttga gctcaactcg tctcaggtaa ttgggcgggt gggtgaattt
     1081 atctctcacc gcctgcaaaa ctatgaccct caaatcacta tcgtgcctgc acgtaagcgc
     1141 ggcacagcat tcagcccgga cgatcctgat ttgatcgaac cactctatga agctgatatg
     1201 attttcatgg gtccgggtag ccctacttat gctgtccggc agttgagagg tagcctggct
     1261 tggcatgccc tgctcgcccg gcaccgcctt ggggcgacca ttgtctttgc cagcgcggct
     1321 accgtggctg ccagcctgtt ttctatacca gtgtacgaga tttacaaggt cggcgaagag
     1381 ctgcattgga aggatgggct ggattttttt ggcctgtacg ggctaccatt ggttttcgtc
     1441 ccccattgga acaacaacga tggaggagaa gagctggata ccagccgctg cttcatgggc
     1501 aagccacgct ttgctgagtt gatggaaatg attccctcag agctgtccat actgggaata
     1561 gatgagaaga ctgccctggt tatggatccg cagtcgtgcg cgtgccgggt gatcgggctt
     1621 ggcggggtga cgatcatcca tgccggcccg gcgcatcgca gtgcaaccca acccagccag
     1681 ctggatggta ccggcttgaa cgaggttgcc gaaaatcgca aggcgcacgt tcaccattac
     1741 cagcatggtc aggccttttc tttgaaaagg attggcccgt tctgtgatcc agaagctggc
     1801 gtggggctac ctggcgaggt ctggcgacgt gccctggaag tccaccatgc gcggcagagc
     1861 caggaaatgg cttcgtttga agatgagtca ccccctcctg aggtgctcgt cctggtggaa
     1921 gagcgccagg ctgctcgggc gcgcaaggat tgggctgcct ccgatagctt acgcgaacag
     1981 gtggctgacc tgggctggct gatccaggat acgccggagg ggccgaaggt ggtcaaaagc
     2041 gacgattaat ttccgaattt ttgatgacaa taaggagtca ctatgacgca aacgctttcc
     2101 gttggttaca tcggcctagg gctgatgggt aaatccattg cccgcaatat ccttaaagca
     2161 ggtttcccgc tggtggtgca taaccgaagt caggcagcag tcgaagagct ggtcgctgaa
     2221 ggggcggtgc gggctggctc ggcggcggag gtagcgtcta gcgtggacgt ggttttcacc
     2281 aatctgccgg atacccctga cgtagagaaa gtggctttgg gttcaaatgg ggtcattgag
     2341 ggtgcacatc cggggctggt cttcgtggat aactccacga ttcagccggc tgcggcgcgg
     2401 cggattgccg aggtacttgg tgaaaaaggg gtgcagtgcc ttgacgctcc ggtgagcggc
     2461 ggggacattg gcgctcgcca ggggacgttg gcgattat
//
LOCUS       DBNHOH010000177         1978 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1100172, whole genome shotgun sequence.
ACCESSION   DBNHOH010000177 DBNHOH010000000
VERSION     DBNHOH010000177.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1100172"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="AC16V7_03210"
     CDS             <1..759
                     /locus_tag="AC16V7_03210"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6256159.1"
                     /translation="NYQLVFGLGALGAMMSTYHLSRLRLPPEPPVRVGKLIQDLARPG
                     ILRFPDGLRSSVGLRFLTRAGDKPLLQLEILRTPFGLLIAGYLFFYITQYMGVPIYPI
                     YFVRGLNLTDGQISLGTALFYGTMLLTSIRLGPISDRLGFRVVLVSGAVCYGLYPLLI
                     AIDGLATYLLGSLVGVISWALAYGGLVNRLMERTPENKRPFHMSFHNLALNFGILGGS
                     LLGSLLADWVGLRPSLWVVAGLRVLAGLILALWA"
     gene            945..1676
                     /locus_tag="AC16V7_03215"
     CDS             945..1676
                     /locus_tag="AC16V7_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256160.1"
                     /translation="MIKKIAWLAGLILIIVSACSGSGPESLPADSHGGGGTPTPISNL
                     PGQVEEDVKPPERDRFDPEQFSEAIGNYVLRPEDLPNDYRIPQGGEHRLSNLGVIQDR
                     GEVEGKHYIVDTGRVDGWHLELERKKKEDIAPGTMASAIELFESSEGAELALSRDWFK
                     AYREENKTPNWIEDGCSFGDKCLFYYYQSIDPATNLTKLEYDIAFVYRNVMVWVMGRG
                     LDIDVTPDYILNAAEALFMRLKKAS"
     gene            complement(1671..>1978)
                     /locus_tag="AC16V7_03220"
     CDS             complement(1671..>1978)
                     /locus_tag="AC16V7_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012616.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HIR6256161.1"
                     /translation="IPAGAFYPAPTVESAVVRVDIYATPRIPAPQMDAFFRLTKAGFS
                     QKRKTLSNALAASLAWPKQEVVAMLLAAQIDPRRRAETLSLEEWNSLTQVYTDEVDL"
BASE COUNT          441 a          478 c          546 g          513 t
ORIGIN      
        1 aattaccagt tagtcttcgg acttggagca cttggcgcca tgatgagcac ctatcacttg
       61 tccaggttgc gccttccccc cgagccaccc gtgcgtgtgg gcaaattgat acaagatctg
      121 gcccgtccgg gaatattgcg atttcctgat ggcttgcgtt cgtcagttgg attgcgcttc
      181 ctcacccgcg ccggcgacaa acctctgctc cagctggaga tattgcgcac tccgtttggc
      241 cttttgattg caggttattt attcttttac atcacccaat acatgggggt accgatatat
      301 ccgatttatt ttgtgcgcgg tctaaattta actgatgggc aaattagcct tggtacagcc
      361 ctcttttacg gcaccatgtt gttgacttca atcaggctgg ggccgatcag cgatcgccta
      421 ggctttcgag ttgtattggt gagtggggca gtatgttatg ggttgtatcc attattaatc
      481 gccatagacg gcctcgccac ctatttgctc ggctcgctgg ttggggtaat cagttgggca
      541 ttagcgtatg gggggctggt gaatcgattg atggagcgca cgccagaaaa caagcgaccg
      601 ttccatatgt cgttccataa tcttgcgctc aatttcggca ttttgggtgg ttcactcctc
      661 ggatctctgc tagcggattg ggtgggattg cgtccttctc tctgggtcgt tgctgggttg
      721 cgtgtcctag ctggattaat attggcgctt tgggcgtaat gccattgggg gcgagagact
      781 gctgacaatc ttgataggca acctcgctag aggtactttt tccctattgt tcgaggcata
      841 caggcaatat acgatatgta agactggcgg ttagcaggcg gcgcttaccc tgctcctggc
      901 taataagttc aacacatacc tgcacaccca acaaggagga tttgatgata aaaaaaattg
      961 cctggcttgc cggtttgatc ttgattatcg tttccgcctg ttcagggagt ggacccgaat
     1021 cgcttcccgc agactctcat ggaggaggtg gcacgccaac gcctatctcc aatttaccgg
     1081 gtcaggtcga agaagatgtc aagccgccag aacgcgatcg atttgaccct gagcagtttt
     1141 ctgaagccat tggcaactat gtccttcgcc ctgaagatct gccaaacgac taccgcatac
     1201 cacagggagg tgagcaccgt ctctccaact tgggggttat acaggatagg ggtgaagtgg
     1261 agggcaaaca ctacattgta gatacaggcc gcgtagatgg ctggcatctg gagttggagc
     1321 gcaagaagaa agaggacatt gccccgggga caatggcgag cgccatcgag ctttttgaat
     1381 ccagtgaggg ggcagagctg gcattgagca gggattggtt caaagcctac cgagaggaaa
     1441 acaaaacccc aaattggatc gaggatggct gcagctttgg cgacaagtgc ttgttttact
     1501 actatcagag cattgatccg gcaactaacc tgaccaagct ggaatatgac attgcttttg
     1561 tctatcgaaa cgtgatggtc tgggtaatgg gccgcggtct ggatattgac gtgacgcctg
     1621 attacatatt aaatgcggcc gaggcactgt ttatgcgctt gaaaaaagcc tcataaatcg
     1681 acctcatctg tataaacctg ggtcagcgaa ttccactctt ccaggctcag ggtctctgcc
     1741 cttcggcggg gatcaatttg cgcggcgagc aacatggcca ccacctcttg tttaggccag
     1801 gccaggctcg ccgccaatgc gttactaagg gtttttcgct tttggctgaa gccagccttc
     1861 gtaagtctaa aaaaggcatc catttgtgga gctggaatac gcggggttgc gtatatgtcg
     1921 acgcgcacta cagccgattc gaccgttgga gcaggataga aagccccagc ggggatgc
//
LOCUS       DBNHOH010000178         2301 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_908195, whole genome shotgun sequence.
ACCESSION   DBNHOH010000178 DBNHOH010000000
VERSION     DBNHOH010000178.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_908195"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="AC16V7_03225"
     CDS             complement(<1..860)
                     /locus_tag="AC16V7_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6256162.1"
                     /translation="MTFFLQNVMNALQWGSFYALIALGYSMVYSILMLFNFAHGDIFM
                     TGSYFGFFISSALVGLSITGTLPLPSWLLLVLTILFTMIATAFLGMIVERVAYRPLRD
                     APRASAAITGLMVGIILETGNLALLGAQRRNYPTLIETQTYLIGGVTITSTRVMIIAL
                     SILLMVALHLFIRRTKWGMAIRAMAYDYVVLPLMGVSSNSIAVMTFALGSALAAAAGI
                     FFGIAYPVLEPYMGILIGWKAFVAAILGGRGSIPGAALAGFLLGFIEIFVAAIFPSTV
                     RDLIAYSIILV"
     gene            complement(986..2131)
                     /locus_tag="AC16V7_03230"
     CDS             complement(986..2131)
                     /locus_tag="AC16V7_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6256163.1"
                     /translation="MLIALALFTVGCGGAAPQTIKIGINAEITGDIPKVGEGTKYAAE
                     MWLEDINNAGGLEVGGKKYPVELVIEDNESKAESAVAANTKLITQDGVLVIVGPQASK
                     QAVPAGEVANNNETPMISPWSTNPRTTLDRPWVFRACFLDPFQGPVNAKFITDEFGFT
                     KAAVLYDVGSDYPKGLAEYFKQAWEELHGAGSVVAFETFTTKDKDFSAQLTKILNSGA
                     EVLFTPQYYDEVPLIVKQAHELGWDKPIVGSDSWGSAELMNLCGDDCKGLFFSTHYAA
                     AGAVGATKAFIDRYNTTYNYVPDDVGALTWDAMMIVQRAIQDCGTITGDLKTDRKCVR
                     DSLAKIQDFAGITGNMTFTEDGDPVKCAVIVKISDAGEFEFYDQACP"
BASE COUNT          588 a          589 c          573 g          551 t
ORIGIN      
        1 acaagaatga ttgagtaagc gatgaggtca cgaaccgtag aaggaaagat tgctgccacg
       61 aagatttcaa taaaccctag aaggaagccg gctagagcag ctccaggaat agagcctcgc
      121 cctccgagaa ttgccgccac aaatgccttc catccgatta gaatgcccat gtagggctct
      181 aaaactggat aggcgattcc aaaaaagatc cctgcagctg cagccagcgc cgatccaagg
      241 gcaaaagtca tcacagcaat tgaattgctg gatactccca tcaatggcag aacaacataa
      301 tcgtaggcca tcgcacgtat tgccattccc cacttggtgc gccggatgaa cagatgcaat
      361 gctaccatta ataatattga aagtgcaatg atcattacgc gtgtgctggt aatggtcact
      421 cccccgatca ggtaagtctg tgtctcgatc aaggtggggt agttgcgacg ttgggcccct
      481 agaagggcga gattgccggt ctccaatata atgcccacca tcaagcctgt gattgcagct
      541 gaagcacgag gagcgtcgcg taaaggcctg taagccacgc gctcgacgat cattccaagg
      601 aaggctgtgg caatcatggt aaataatatt gtcagtacca ggagcagcca actgggcaga
      661 ggtaaagttc ctgttatcga aagcccaacc aaagcactag agatgaaaaa cccgaaatag
      721 cttccggtca taaagatgtc accgtgggcg aaattaaaca gcataagaat gctgtaaacc
      781 atcgagtagc ccaaggcgat taaggcatag aaactacccc attgcaatgc attcatcacg
      841 ttttgaagga aaaacgtcat ctcgatggct cctcttatcg agagggcgga cattatcgaa
      901 tgccgccctc tcattggtca attagcggtg cacaagtgat cgatagatta cgctaggatc
      961 caggctatct ttcctatgca tatatttaag ggcaggcctg atcgtaaaat tcgaattcac
     1021 ccgcgtcact gatttttacg ataacagcgc acttgactgg atcgccatcc tcagtgaagg
     1081 tcatgttgcc cgtaatgcct gcaaagtctt ggatcttggc taacgaatcg cgtacgcact
     1141 tccggtctgt ttttaagtcg ccggtaatcg taccgcaatc ctggatcgcc ctttgcacaa
     1201 tcatcattgc atcccatgtc agagccccta catcatccgg gacatagtta taggtggtgt
     1261 tgtagcggtc aatgaacgcc ttggtcgcgc cgaccgcgcc agccgcagca tagtgagtgc
     1321 tgaagaaaag acctttgcag tcatcaccgc ataggttcat cagctcggct gagccccagc
     1381 tatcgctgcc tacgatgggc ttgtcccacc ccaattcgtg agcctgcttc acgatcaagg
     1441 gaacctcgtc ataatattgg ggagtgaaga ggacttcggc tccagagttg aggatcttgg
     1501 tcaattgcgc gctgaagtct ttatccttgg tcgtgaaggt ctcaaaggcc acaacagagc
     1561 cagctccgtg aagctcttcc caggcttgtt tgaagtactc ggccaagccc ttggggtagt
     1621 cgctgcccac gtcgtaaagt acggcggcct tggtgaagcc aaattcatcg gtaatgaact
     1681 tggcgttcac cggcccctgg aagggatcca ggaagcaggc gcggaagacc cagggacggt
     1741 ctaaggtagt gcgggggttg gtggaccacg gactgatcat aggggtctcg ttgttgttgg
     1801 ccacttcgcc ggcaggcacc gcctgcttag aagcctgggg accaacgatg acaagcacac
     1861 catcctgggt gataagcttg gtattggccg ctacagcgga ttcggcttta gattcgttat
     1921 cttcgatgac cagctcaacc ggatattttt tcccaccaac ctccaatccc cctgcgttgt
     1981 tgatgtcttc aagccacatt tcagcggcat atttagtgcc ttcgccgact tttgggatat
     2041 cgcctgtgat ctcagcatta attccgatct ttattgtctg gggtgctgcg cctccgcatc
     2101 ctactgtgaa gagcgccagc gcaatgagca tggcagtgaa tttaaaacaa gtattctttc
     2161 tcatagctcc tcatcctcca taaagtagtt gatctatgcc agcatcgatt gaaaaacttt
     2221 gaattattcg agacagagat tcgtcgctct gggcaattgc tatacatcac ctcctttgga
     2281 taatccattc cgtggagctt t
//
LOCUS       DBNHOH010000179         2729 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1484395, whole genome shotgun sequence.
ACCESSION   DBNHOH010000179 DBNHOH010000000
VERSION     DBNHOH010000179.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1484395"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="AC16V7_03235"
     CDS             complement(<1..786)
                     /locus_tag="AC16V7_03235"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR6256164.1"
                     /translation="MTTRIQWIQDEIKKLKDAGLYNRIRTLSTPQGAWLVVDGKRTLN
                     FCSNNYLGLANHPRLVQAAKDAVEKFGVGPAAVRSIAGTMELHIQLEQRLAAFKGVES
                     AITFQSGFNANLGSIPALVGKGDGIFSDELNHASIIDGCRLSGAQIVRYNHCDAKDLE
                     KALIQQRGKFTRAMVITDGVFSMDGDIAPLDKIYEVCQDHNVLLMVDDAHGEGVLGRG
                     GRGIVDHFRLHGKVDIEVGTLSKAFGVVGGVVAGNPTVVEWLRQ"
     gene            complement(803..1798)
                     /locus_tag="AC16V7_03240"
     CDS             complement(803..1798)
                     /locus_tag="AC16V7_03240"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HIR6256165.1"
                     /translation="MTTQITIVGLGQIGASIGLALAEQEQLFQRIGHDIHLETARRAQ
                     KMGAIDKVAINLPSAVREADLVILSVPADQLRETLEIIAQDLKENAVVMDTGPIKVSM
                     SEWIKELLPDNRFYVGLVPSINPAYLYQEEIGIDAAHADLFQRGIMAIITPPNTSSDA
                     LQLASKLASLLGSDPLFVDPLEIDGLLAATHALPQLLAVALLNATVSQPGWREARKIA
                     GRAYADVPAANLHGGMADGLGGMTLHNRDNVVRVLDRAIASLLSLREAIEANDGEFLV
                     KQLESAQQGYAEWLKERLAANWAFKGMPESVSKSSGRVDFLSRLLGSGVKRKDRK"
     gene            complement(1811..2353)
                     /locus_tag="AC16V7_03245"
     CDS             complement(1811..2353)
                     /locus_tag="AC16V7_03245"
                     /inference="COORDINATES: protein motif:HMM:NF014069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR6256166.1"
                     /translation="MIAERILYRARQFWNALFSAPSLEDLETISPLLNPAQMALFSQM
                     HRSEQVHSIKVMRTVMAASDGISAEQRRNLLQAALLHDVGKNYYPLRLWERVLIVLAK
                     TLTPDRVKDWGEGEARGWRRAFVIARQHPAWGAQMAAEAGASPLTVSLIQRHQDLLSE
                     AGEALEDQLLRLLQSADHEE"
     gene            complement(2350..>2729)
                     /locus_tag="AC16V7_03250"
     CDS             complement(2350..>2729)
                     /locus_tag="AC16V7_03250"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR6256167.1"
                     /translation="VSLAGPLSNLLLAIVAAIPFRLGVFSVLDAQMALFTARDQFLPT
                     LPQLLYIFIQINLVLLLFNLIPLAPLDGEKIAEYFLPASWLRTLDRIRPYGPVILMVI
                     VFFGVLTYIITPPLRLLMNLLVG"
BASE COUNT          670 a          773 c          663 g          623 t
ORIGIN      
        1 ctggcgcagc cattccacaa ccgtcggatt gccagctacg acgccgccca ccacaccaaa
       61 ggccttcgaa agcgtgccca cctcaatatc cacttttcca tgcaggcgaa agtgatcgac
      121 gatgccgcga ccaccccgtc caagcacgcc ctcaccgtgg gcatcatcca ccataagcaa
      181 cacattgtga tcctgacaga cttcgtaaat cttatccaga ggggctatgt caccatccat
      241 gctgaagacg ccgtcagtga tgaccatggc gcgcgtaaat ttaccgcgct gctggatgag
      301 cgccttctcc aaatcctttg catcacagtg attgtaacgg acgatctgag cccctgagag
      361 gcggcaacca tcgatgatgc tggcatgatt caattcatcg gagaaaattc cgtctccctt
      421 acccaccagc gccgggatac tacctaaatt tgcgttgaac ccagactgaa aggtgatagc
      481 cgattcaacg cctttgaaag cagccaggcg ctgctccaat tggatatgca attccatcgt
      541 cccggcgatg ctacgcaccg cagccggtcc cacgccaaat ttttccacag catctttggc
      601 cgcctgcacc aagcgtgggt ggttagccag ccccaagtag ttattcgaac aaaaatttaa
      661 tgtgcgctta ccgtccacca ccagccaagc cccttgcggt gtgctgaggg tgcggatgcg
      721 gttgtaaagc cctgcatctt ttagtttttt aatctcgtct tgaatccatt gaatcctggt
      781 agtcatggtt ttccctcgca atttatttac gatccttccg cttgaccccc gaaccaagca
      841 agcgggatag gaagtcaact ctccctgacg atttcgatac agattcgggc atgcctttaa
      901 aagcccagtt ggcggccagt cgctctttca accactcagc ataaccttgc tgggcagatt
      961 ctaactgctt cactagaaac tcgccatcat tcgcttcgat ggcttctcgc agcgatagta
     1021 atgaggcgat agccctgtct aggacacgaa ccacattatc acggttgtgc aatgtcatgc
     1081 cacccaagcc atctgccata ccgccatgca ggttggcagc aggtacatcg gcataagccc
     1141 gcccggcgat cttgcgcgcc tcacgccaac ccggctgact aactgtggca ttaagcaacg
     1201 caacagccaa cagttggggt aaagcgtgcg tggcagccag taagccgtca atttccaatg
     1261 ggtcgacaaa caggggatct gaacccagca ggctcgccag tttgctggct aactggaggg
     1321 catcgctgga tgtattgggt ggggtgataa tcgccatgat accacgctgg aataagtcag
     1381 catgcgcggc atcgatcccg atctcttctt gatataaata agccggattt atagatggta
     1441 ccagccccac atagaagcga ttgtctggta gcaactcttt tatccactca gacatcgaaa
     1501 ccttaatcgg accggtatcc atcaccaccg cgttttcctt tagatcctgg gcaatgattt
     1561 ctaaggtctc gcgcaattgg tctgcaggta cagacaatat aaccaggtcc gcctcgcgca
     1621 ccgcggacgg caaattgatg gcgactttat caatggcacc cattttctgg gcgcggcggg
     1681 cggtctccag gtgtatatcg tggccaattc gctggaacaa ctgctcctgc tcggccaagg
     1741 ccaatcctat tgatgctcca atctgtccta aacctacaat ggttatttgg gtcgtcatag
     1801 ataaattatc ctattcctca tgatctgcgg attgtaataa tcggagtaat tgatcttcta
     1861 acgcctcccc ggcttcggaa agaagatctt gatgtctttg tatcagagaa accgtcagcg
     1921 gcgaagctcc agcctctgct gccatttgag ctccccaggc ggggtgctgg cgggcgatca
     1981 cgaatgcacg ccgccaacca cgcgcttctc cttctcccca gtctttaacc ctgtcgggtg
     2041 tcaacgtctt cgctaagacg atcagaaccc gctcccacaa cctgagtgga taataatttt
     2101 tgcctacatc atgaagcagg gccgcttgca gcaaatttct tcgctgctcg gctgagatcc
     2161 catcacttgc agccatcaca gtccgcatca ccttgatgct gtgcacttgc tcgctgcggt
     2221 gcatctggct aaagagcgcc atttgcgcgg gattgagtag cgggcttatc gtctcaagat
     2281 cctcaagcga gggcgccgaa aacagggcat tccagaactg tcgggcgcgg tataggatac
     2341 gttcggcaat catcccacca gtagattcat caacagcctg agtgggggtg tgataatgta
     2401 agtcagcaca ccgaagaaca caatgaccat caggatgacc ggcccgtatg gacggatgcg
     2461 gtcgagggtg cgcaaccaag aagctgggag gaagtactcg gcaattttct ccccatccaa
     2521 aggcgccagt gggattaagt tgaacaggag caataccaaa ttaatttgga taaatatata
     2581 tagaagttgt ggtagcgtgg gtaaaaattg gtccctggca gtaaacaacg ccatctgtgc
     2641 atccagcact gaaaaaacac ctaaacgaaa cgggatggct gccacaatcg ccaggagtaa
     2701 attcgagagc gggccagcta acgagaccc
//
LOCUS       DBNHOH010000180         2679 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1484399, whole genome shotgun sequence.
ACCESSION   DBNHOH010000180 DBNHOH010000000
VERSION     DBNHOH010000180.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1484399"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="AC16V7_03255"
     CDS             complement(<1..822)
                     /locus_tag="AC16V7_03255"
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HIR6256168.1"
                     /translation="MSKKIYTFTILVIILAMITPMSVFSQGAADSVQITLLHTNDFHG
                     RLEADSSGRGGSANLASVINNIRAAEGDENVALLDAGDVYFAAPAVSQLLMGESTIDI
                     YNMMGYDLAVFGNHEFDKGQAELALRVDQSDFPWLGANVVLEGTDWDLPPWAEPYKIM
                     TVGEAPNQATLGVLGLAGEETPEVTLLGTTDGLVFKDLTETILHYYDEVLAQADALVV
                     VVHMGTQDSGPYKGLVTVAQELIDAGKPVDLMIGGHQHQALFAPVYVGDTAIVSAG"
     gene            1282..1413
                     /locus_tag="AC16V7_03260"
     CDS             1282..1413
                     /locus_tag="AC16V7_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256169.1"
                     /translation="MSVTNSLDLRVDLPIEWEDADPLARRGKNGGRIPGETLFLTRA"
     gene            complement(1492..2490)
                     /locus_tag="AC16V7_03265"
     CDS             complement(1492..2490)
                     /locus_tag="AC16V7_03265"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6256170.1"
                     /translation="MDSELLAEMMVAILERGYEIDSVSIIRNGYLVADVSVYPFRPNT
                     KHVIHSCTKSIVSILIGIAIEQGYIEGVQQPILSFFPGKTVANLDARKEAMTLEQVLS
                     MATGLNCRDSYLYRWQGLNQMRESDDWVQFMLDLPMVEAPGTRFEYCNGASYLLSAII
                     KETTGVSALEFAQEHLFGPLGISDVVWPSSPQEVNIGWGEIRMLPLDMAKIGILYLNG
                     GRWEGKQIVSTAWVEESTREHISATLEDGYGYQWWVDDSGIFMALGYAGQFIFVIPDK
                     EMVVVFTSDLSEEDFYVPQTLLNDFILPATKSSAALPDNPSGVALLESRIAELASP"
BASE COUNT          670 a          742 c          638 g          629 t
ORIGIN      
        1 gccagcagaa acgatggcgg tgtcgccgac gtaaactggt gcaaacagcg cctggtgctg
       61 gtgcccgccg atcatcaaat ccaccggttt cccggcgtcg atcaactcct gggccaccgt
      121 aaccaacccc ttgtacgggc cgctgtcctg agtgcccatg tggacgacca cgaccagcgc
      181 gtcggcctgc gccagaacct cgtcatagta atgcaggata gtctcggtta ggtccttaaa
      241 taccagacca tcggtggtgc cgagcagggt gacctccggg gtttcctcgc ccgccagacc
      301 aagcacaccc agcgtcgcct gattaggagc ttcgccaacg gtcatgatct tgtatggttc
      361 agcccagggt ggcaggtccc aatcggtgcc ctccaagacg acgttggctc ccagccaggg
      421 gaagtcggat tggtctacgc gcaaggctag ttcggcttgg cctttatcga actcgtggtt
      481 gccgaacaca gccaggtcgt agcccatcat attgtagatg tcgatggtgc tctctcccat
      541 caacagttgc gagacagccg gtgcggcaaa atatacatcg cctgcgtcca gtagcgccac
      601 attctcatcg ccctcggcgg ctcgtatgtt gttgatcacg ctcgccaggt tggcagagcc
      661 gccgcggcca gaagagtcgg cctccagccg cccgtgaaag tcgttggtgt gcagcagcgt
      721 gatttgcaca ctatccgctg ctccctggct gaacaccgac atcggtgtga tcatagccag
      781 gatgatcact aagatagtga atgtataaat tttctttgac atggctctcc tctttttgaa
      841 tagaagtcgt ctcgtaatca atccaatctt aaatcagtga cctatagtta ttcgtattat
      901 ccagcctcct ttgtgactaa gatcagttgt ttcttctcgt ggttagaaac catcaaaaac
      961 aaatcgccca ccacttgtgt gatgcgcgac catacacaaa gagaattcgc ctgtaagata
     1021 tttatcaagg tcatgaagag cgttaagagg gtgatgaacc tgtgtgcgcc ctaataacca
     1081 cagccaagca ataacaccca agcgatcatg tgcaggcgct atcctgataa acctgaaatc
     1141 agcgcacaac cttagcagaa aaaagaatat ttgatttaac ttttccaccc caccccccta
     1201 catccccaac acagatgcag gcacgcttta tcataataag aataataaaa taattcgatt
     1261 ttgcgtagtg acaatcatca tatgtctgtc actaactcgt tggatttgcg cgtcgatctt
     1321 ccgatcgaat gggaagatgc tgatccgctt gcccgtcgcg gcaaaaacgg cgggcgcatt
     1381 cctggcgaga cgctcttttt gactcgtgcg taatggtagc tttggagata gtaaaaatcg
     1441 tattggaact aatcggcttt gtggggcgag atagagtatg cgaggtgaac attatggact
     1501 ggctagctct gcgatgcgcg attcaagcaa tgctacgccg gagggattgt caggcaatgc
     1561 agccgacgat ttagttgcag gcaggataaa gtcgttcagc aaggtttgag ggacataaaa
     1621 atcctcctcg ctcagatcgc tggtaaatac caccaccatc tccttatctg ggataacaaa
     1681 gataaactgc cccgcatatc ccagtgccat gaaaatacca gaatcatcca cccaccactg
     1741 atacccatag ccatcctcaa gggtcgccga tatgtgctcg cgggttgact cctccaccca
     1801 tgcagtagag acgatttgct taccttccca tctcccgcca ttcaagtaca gaatcccaat
     1861 cttcgccata tccagaggca gcatgcgtat ttcaccccac ccgatattaa cctcctgtgg
     1921 gctggatggc cagaccacgt ccgagatccc caatggccca aagagatgct cctgggcaaa
     1981 ttcaagagcg ctcaccccgg tcgtttcctt aataatagcg gacagaagat acgaagctcc
     2041 attgcagtac tcgaaccgcg ttcctggggc ttccaccata ggcaggtcca gcataaattg
     2101 aacccaatcg tcactttctc tcatctggtt cagtccctgc cagcgataga gatacgaatc
     2161 gcggcaattt agccccgtcg ccatgctcaa gacctgctcc agcgtcattg cctctttgcg
     2221 tgcatccaga tttgcaactg tcttccccgg gaaaaagctc aaaatgggtt gttgcactcc
     2281 ctcgatatac ccttgctcga tggcgatacc aattaatata gagacaatgc tcttggtaca
     2341 ggaatggatg acgtgcttcg tattcgggcg aaacgggtaa accgaaacat ctgccaccag
     2401 gtagccattc cggataattg aaacgctgtc aatttcgtag ccccgctcca ggattgccac
     2461 catcatttct gctaacaatt ccgaatccat gccttgttgc tcaggcgtag aggtccgaaa
     2521 acccttggtt ggccagtatt cgacaaattc ctgtgtcgca atcggtgcca cggcgggtgt
     2581 aacttgagcg catccagaaa ggaacgcgag gatacaaaac agaagaagag atctgctgat
     2641 ttttaggttg aagatcatca cacccccgga gttctagct
//
LOCUS       DBNHOH010000181         1391 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1741649, whole genome shotgun sequence.
ACCESSION   DBNHOH010000181 DBNHOH010000000
VERSION     DBNHOH010000181.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1741649"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..1137
                     /locus_tag="AC16V7_03270"
     CDS             118..1137
                     /locus_tag="AC16V7_03270"
                     /inference="COORDINATES: protein motif:HMM:NF014841.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HIR6256171.1"
                     /translation="MNDTIEVLIALPFPDKLIERLTEISPRLKFTVKSPNKPDDISPD
                     LWAHTEVLYTARVLPTAELAPNLRWIQFHWAGVDHAIDDPILRKPDLVATTLSGAAIS
                     QMAEYAVMMLLALGRHMPELVTLQNRSEWPHDRWERFTPIELRGSTVGIIGYGSIGRQ
                     LARLLSYFGATVLATKRDAKHPQDSGYIPEGMGDQGGDYVRRLYPAQALRTMLKACDF
                     VVVCVPKTPGTINLIKAEELAAMKPSAYLVDVSRGGIVDQQALLAALKDHKIAGAALD
                     VFPEEPLPAKSPLWKLPNVLITPHISGITSHYDDRAVELFSANLLRYLEGQPLYNRID
                     PKLGY"
     gene            1139..>1391
                     /locus_tag="AC16V7_03275"
     CDS             1139..>1391
                     /locus_tag="AC16V7_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256172.1"
                     /translation="MSPRRIDPSSAAETARQRQLQAILFDLGSTLIYFDGNWDEIRPQ
                     RDAILLSRLHEAGINLDGDTFLEEFNANLSAYYIERDTEF"
BASE COUNT          335 a          404 c          343 g          309 t
ORIGIN      
        1 cccgatgtcc gcccaccccc cctcctttcc ttattaactg cgtaagtcct aatagaataa
       61 aatttgaggt atgtgtcgcg ctcccacgtg cttcaatttt tgctataatg gctaaccatg
      121 aatgatacga ttgaggtcct gattgcattg ccattcccag acaaactaat cgaacggctt
      181 accgaaattt caccgcgcct gaaattcacc gtaaaaagcc caaacaagcc tgatgatatc
      241 tccccggatt tgtgggcaca cactgaggtg ctatataccg cccgtgttct tcccactgca
      301 gagcttgcac ctaacctgcg ctggattcaa ttccactggg caggcgtcga ccacgctatc
      361 gacgatccca tcctgcgcaa gccggacctg gtggcaacga cactgagcgg tgcagcaatt
      421 tctcaaatgg ctgagtacgc cgtaatgatg ttactggcgc ttgggcgtca catgcccgag
      481 ttagtaaccc ttcaaaaccg tagcgaatgg ccgcacgacc gctgggagcg ttttacaccc
      541 atagaattgc gcggtagcac ggttggcatt attggttacg ggagcattgg gagacagcta
      601 gcgcgcctgt tgtcatattt cggcgctacg gttcttgcaa ccaaacgcga tgcgaagcat
      661 ccacaagatt ccggatatat ccccgaaggc atgggcgatc aaggcggtga ctatgtcagg
      721 cgtctctacc cagcccaagc gctgcgcacg atgctcaaag catgtgactt tgtagtggta
      781 tgcgtgccca aaacgcctgg cacaatcaac ctgatcaagg ctgaagaatt ggccgcgatg
      841 aaacctagcg cctacctggt ggacgtctcc cgaggtggaa ttgtagatca acaagccctg
      901 ctcgccgccc tcaaagacca caaaatcgcg ggggcagccc tggatgtctt ccctgaagag
      961 cctctgccag ccaaaagccc cctctggaaa ttgcccaacg tgctgattac accccatatc
     1021 tccggcatca cttcccatta cgatgatcgc gccgtagaac tgttcagcgc caacctgctg
     1081 cgctatctgg aaggacagcc attatataac cgcatcgatc ccaagctagg atattgattt
     1141 gtctccacgc cgcatcgatc catcctcggc agcagagaca gcaagacagc ggcagttgca
     1201 ggctatcctt tttgacctgg gcagtacgct gatctatttt gacggcaact gggatgaaat
     1261 tcgtccgcag cgcgacgcga tactgctcag ccggctgcac gaggcgggca tcaacctgga
     1321 tggcgacacg ttcttggaag aattcaacgc gaatttgagt gcgtactaca tcgagcgcga
     1381 taccgagttc a
//
LOCUS       DBNHOH010000182         2163 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_587484, whole genome shotgun sequence.
ACCESSION   DBNHOH010000182 DBNHOH010000000
VERSION     DBNHOH010000182.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_587484"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /locus_tag="AC16V7_03280"
     CDS             <1..1127
                     /locus_tag="AC16V7_03280"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF019552.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HIR6256173.1"
                     /translation="MLQLALWVHALPETQRQPFPVATRQSLSAATRWLLELVDGESGR
                     VPNLGPNDGAYIQPLTVCPFRDFRPVLQAAGAAFLGERPYPAGLWDEMSLWLGDVGQG
                     IGNKGDDVRLSSSPVLRIPQQESWAYLRAARFTSRPGHADQLHLDLWWRGINLAQDAG
                     TYLYNAPPPWDNSLSRTGVHNTLSVDGQDQMTRGGRFLWLDWAQADGVAYEKSPDGSW
                     ERMTASHNGYRRLGLVHQRAVTAYQDGRWMVEDSLVRSGKPKVESSKLKPTNFSLHWL
                     LPDWEWEMVERGESSVAIYLASSLGSVLLMMQPEEGTQLTGIQLVRAGELLVGDGAVA
                     PFQGWLSPTYGQKIPALSLTIAAVGTPPCSIVSRWIFETA"
     gene            1206..>2163
                     /locus_tag="AC16V7_03285"
     CDS             1206..>2163
                     /locus_tag="AC16V7_03285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6256174.1"
                     /translation="MHILLIHQAFTALDEPGGTRHHELARYLVTRGHRLTVIASPVSY
                     LTGEARHGRIPWVEWQEDEPGIAILRTYTYPALHRSFVHRVFSFFSFMFSSFLIGFGV
                     RDVDLVWGTSPPIFQGATAWALARLKGVPFLFEVRDLWPAFAVAVGVLRNKVLIRASE
                     WLERFLYRRANLVVVNSPGFVDHVHARGAWQVALVPNGSDTAMFDPRLDGSEFRAAHG
                     LQGKFLAMYAGAHGMSNDLGVVLEAVALLDDQPDIAIALLGAGKEKPALIAMAQAMGL
                     SNLHFLPPVPKGEMPRALAAADACIAILKPIEMYKTVYPNKVF"
BASE COUNT          427 a          570 c          640 g          526 t
ORIGIN      
        1 tgatgctgca acttgcgctg tgggtgcatg cccttcctga aacccaaagg caacccttcc
       61 cggttgctac gcgccaaagc ctgtcggcag ctacccgctg gttgctggag ctggttgatg
      121 gtgaaagcgg gcgcgtcccc aatttagggc cgaacgatgg cgcctatatt caacctctca
      181 ctgtttgccc tttccgtgat ttccgcccgg tgctgcaagc cgccggagct gctttcctcg
      241 gtgagcgacc atatcctgct gggttatggg atgagatgag tctttggcta ggagatgtgg
      301 gacaggggat agggaataaa ggggatgatg tccggctttc gtcctctcct gtattgcgta
      361 tccctcaaca agaatcatgg gcctacttgc gtgcagcacg ctttacctct cgtccgggtc
      421 acgccgacca actgcacctt gacctatggt ggcgcgggat taacttagca caggatgctg
      481 gtacttacct ttataacgcc ccgcccccat gggataattc cctgagccgt acgggtgtcc
      541 ataacacgct gtctgttgat gggcaggatc agatgacacg aggtggtcgt tttctctggc
      601 tggattgggc tcaagcggac ggagtcgctt atgagaaatc gccggatgga tcctgggagc
      661 gtatgaccgc cagccacaat ggctatcggc ggctgggatt ggtacaccag cgagctgtga
      721 cagcctatca agatggtcgc tggatggtgg aagattcgct ggtaaggagc ggaaaaccaa
      781 aagttgaaag ctctaagctg aagcctacaa actttagctt gcattggttg cttccagatt
      841 gggaatggga gatggtagag agaggagagt cgagcgttgc gatttaccta gcgtcatccc
      901 ttggaagcgt gttactcatg atgcaaccag aggaaggaac acagctaaca ggtatccagc
      961 ttgtccgcgc cggtgagctg ctggttggcg atggagcggt cgcgcctttc caaggctggc
     1021 tatccccaac ctatgggcag aaaatcccgg cgctctcgtt gaccattgcc gcggtgggga
     1081 cacctccttg ctcgatcgtt tctcgctgga tttttgagac tgcgtaacaa tatttattct
     1141 ataagtgcta acgaatccca gatattctgg gataatggtt tccagccttc gatcagaaat
     1201 atgctatgca catcctgtta atccaccagg cctttaccgc gctggatgaa cccggcggta
     1261 cccgccacca cgagctggcg cgttacctgg tcactcgcgg acaccggctc accgtgattg
     1321 ccagcccggt ctcctacctg actggcgaag cgcggcatgg tcgtatccct tgggtggagt
     1381 ggcaagaaga cgaaccgggg atcgccatac tgcgcaccta tacctacccg gcgctgcatc
     1441 gcagtttcgt ccaccgcgtg tttagctttt ttagctttat gttttcttcg ttcttgatag
     1501 gctttggtgt gagagacgtg gacttggtgt gggggacgtc gccacccatc tttcagggtg
     1561 ctactgcctg ggcgctggcg cgcttgaagg gcgttccttt cctgtttgaa gttcgcgatt
     1621 tgtggcctgc ttttgccgta gctgtagggg tattgcgcaa taaggtcttg atccgtgcct
     1681 ccgagtggct ggaacgcttt ttgtatcgcc gtgctaacct agtggtggtc aactcaccag
     1741 gcttcgtcga tcacgttcac gcacgtgggg catggcaggt agcgttggtg ccaaatggct
     1801 ccgacacagc catgtttgac ccacggctag atgggagcga atttcgtgca gcgcacggcc
     1861 tgcagggcaa attcctggcg atgtacgctg gggcgcacgg catgtccaac gatctgggtg
     1921 tggtgttgga ggcggtggcg ctgcttgacg accagccgga tatcgccatc gccctcttgg
     1981 gtgcaggcaa ggaaaaacct gctctgatag cgatggctca ggcaatggga ttatcgaact
     2041 tgcatttcct cccgccggtg ccaaagggag agatgcctcg ggcgctggcg gcggcagacg
     2101 cctgcatcgc cattctcaaa cctattgaga tgtacaaaac ggtctatcca aataaagtct
     2161 tcg
//
LOCUS       DBNHOH010000183         2912 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_203032, whole genome shotgun sequence.
ACCESSION   DBNHOH010000183 DBNHOH010000000
VERSION     DBNHOH010000183.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_203032"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..1094)
                     /gene="hypE"
                     /locus_tag="AC16V7_03290"
     CDS             complement(27..1094)
                     /gene="hypE"
                     /locus_tag="AC16V7_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HIR6256175.1"
                     /translation="MLERNEVNIEGAVCPVPLVHKDHIVMGHGSGGKLSHDLIAGMFL
                     PAFNNPILNAGNDAGLAQPNACTKLAISIDSHVVWPLFFRGGDIGRLAVCGTVNDVAM
                     LGATPLYLTASFILEEGLNISLLQKIINSMREAAQEAGVQIVAGDTKVVEKGKADGLY
                     INTSGIGLVKPGVEIGGAHARPGDKVILSGPIGDHGIAVLAARGELGFEAEIMSDVAP
                     LNKLVASILEVSDRVHVLRDPTRGGLATSLNEIARQSKVGILLDESNIPIRPAVQAAC
                     EMLGFDPLYVANEGKLIAIIGKEDADNAVVAMRKQAYGTQTCIIGEVIEAPQERVLMK
                     TSIGSTRIVDTLSGELLPRIC"
     gene            complement(1087..2193)
                     /gene="hypD"
                     /locus_tag="AC16V7_03295"
     CDS             complement(1087..2193)
                     /gene="hypD"
                     /locus_tag="AC16V7_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HIR6256176.1"
                     /translation="MKYVEEYRDGALVQGVINEIRSIITRPWVIMEICGGQTHSIMRY
                     GIDQLLPGEIELVHGPGCPVCVTSLELVDKALAIASMPNVIFTSYGDMLRVPGSGRDL
                     FAVRAAGGDVRVVYSPLDALKIAQQNPEKQVVFFAIGFETTAPANAMSVHQAHAMGVN
                     NYSVLVSNVRVPPAMRAILGSPANRVEGFLAAGHVCTVMGYWEYLPIAEKYKVPIVVT
                     GFEPLDIAQGILMAVKLLEQGKVEVANAYSRVVSQAGNRPAQALINQVFVDCDQKWRG
                     IGVIPQSGWCLRPEYRQYDAAIRFDVGNIQPDESPICIAGQILQGMKKPHHCTAFGKQ
                     CTPENPLGATMVSSEGACAAYYRYSWDRGAMVNA"
     gene            complement(2237..2473)
                     /locus_tag="AC16V7_03300"
     CDS             complement(2237..2473)
                     /locus_tag="AC16V7_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HIR6256177.1"
                     /translation="MCLGVPGKIIEIYEVNGLQMGKIDFGGVTREACLAYVPEAQLGD
                     YTIIHVGFALNLISEQEAQETLAILREIADLEQE"
     gene            complement(2482..>2912)
                     /locus_tag="AC16V7_03305"
     CDS             complement(2482..>2912)
                     /locus_tag="AC16V7_03305"
                     /inference="COORDINATES: protein motif:HMM:NF046761.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Kae1-like domain-containing protein"
                     /protein_id="HIR6256178.1"
                     /translation="YEAQAAFEFETKADHHEQGAYSFEYLKQDDNTSGPLIIANPASL
                     FIAMVEDLRTSQPIDLISARFHNAVANLVHEVVREISRQFGLQDVALSGGVWQNMNLL
                     YKTVALLQDDGYRVFIHNKVPANDGGLALGQAIIAAQAFK"
BASE COUNT          703 a          768 c          703 g          738 t
ORIGIN      
        1 ctatggcttg atcgcccaga ttaatttcaa caaattcttg gtagcaactc gccgcttagc
       61 gtatcgacga tgcgcgtgct accgatggat gttttcatta gaacgcgctc ttggggggct
      121 tcaattacct cgccgatgat gcaagtctgt gtgccataag cctgtttgcg catagccaca
      181 accgcgttgt ctgcatcctc tttgccaata attgcaatca gcttcccttc gttggcaaca
      241 tacaaggggt cgaagccaag catctcgcaa gctgcttgga ctgctgggcg aatagggatg
      301 ttggattcat ctaacaaaat tccaactttg gactggcgcg cgatctcatt caggctggtg
      361 gctaatcctc cgcgggtggg atcgcgtaaa acatgtaccc tatcactgac ttccaaaatg
      421 gaagctacta atttattgag tggagctacg tcgctcatta tctccgcttc gaagccaagt
      481 tcgccacgtg ctgctagaac cgcaattcca tgatcgccaa tcgggccgga gaggatcact
      541 ttatccccag ggcgagcgtg cgctccaccg atctccacgc caggcttgac caagccaata
      601 ccgctagtgt tgatatagag gccatctgct ttgccttttt ctacaacttt ggtgtcccca
      661 gcgacaatct gcacacctgc ttcttgggca gcttccctca tagaattgat gattttttga
      721 agaaggctga tgtttagccc ctcttccagg ataaaactgg ctgtaaggta gagaggggtt
      781 gctccaagca ttgctacatc attgaccgta ccacaaactg ccagcctgcc aatgtcgcct
      841 ccgcggaaaa acaatggcca aaccacatgc gagtctatgc tgatggctag ttttgtacaa
      901 gcattaggtt gagccaggcc cgcatcattc cctgcgttta atatgggatt gttgaacgcc
      961 ggcaggaaca taccagcaat cagatcgtga gatagcttgc cgccgctgcc atgacccatg
     1021 acaatatggt ctttatggac taagggcact ggacatactg ccccttctat attgacttca
     1081 ttgcgttcaa gcattaacca tcgctcctcg atcccaggaa tagcgataat acgccgcaca
     1141 agcgccttca gacgagacca ttgtggctcc aagaggattt tccggtgtgc attgtttgcc
     1201 aaaggccgta cagtggtgtg gctttttcat tccctggagg atttgaccgg cgatgcagat
     1261 tggagactcg tcgggttgaa tatttcctac gtcaaaacga atagcggcgt catactgacg
     1321 atattcggga cgcagacacc aacctgattg aggaataacc ccgatccccc gccatttctg
     1381 gtcacaatcg acgaaaacct gatttatgag cgcctgggca gggcggttgc ctgcctggga
     1441 caccacgcgt gagtaagcgt tagctacttc caccttgcct tgttcaagaa gctttaccgc
     1501 catcaatatt ccctgggcaa tatctagcgg ctcgaagccg gtgacaacga tgggcacctt
     1561 atatttttcg gcgataggaa ggtattccca atatcccatt acagtacaga catgtcctgc
     1621 tgccaggaat ccttcaacgc gattagcggg tgagcccaat atagcgcgca tggcaggggg
     1681 aacgcgcaca ttcgagacga gcacgctata gttgtttacg cccatcgcgt gtgcctggtg
     1741 gacgctcatg gcgtttgctg gcgcggtggt ttcgaaacct atggcgaaga atacaacctg
     1801 cttttccggg ttttgctggg cgatcttaag ggcatctaat ggtgagtaca cgacgcgcac
     1861 atcgccgcca gctgcgcgca cggcgaatag gtcgcgcccg gagccaggta cccgaagcat
     1921 atctccatat gaagtaaaaa ttacgttcgg catggaggca atagccagtg ctttgtcaac
     1981 taactccaag gaagtaacgc agactggaca gccaggtccg tgtacaagct caatctctcc
     2041 agggagtaac tggtcaatgc cgtagcgcat aatagaatga gtttgtccac cgcagatttc
     2101 cataataacc caaggacggg taataatact gcggatctcg ttgatcacac cctggacgag
     2161 tgcgccatca cggtactctt caacgtattt catgtcaacc tttttgcctg agcggttttc
     2221 cgcttgctca aagaccttat tcttgttcta agtcggcgat ttcgcgcagt attgcaaggg
     2281 tctcttgggc ctcctgctcg ctgatcaaat tcaaggcgaa accgacatgt atgatggtgt
     2341 aatcgcctag ctgcgcttca ggcacatagg caagacaagc ttcccgtgta acgccgccaa
     2401 aatcaatctt gcccatctgc aggccattga cttcataaat ctctattatt tttcccggta
     2461 cacctaaaca catgctgcct cctatttgaa cgcttgagct gcaattattg cctggccaag
     2521 tgcaagtcca ccgtcattcg caggaacttt gttatgaata aagacgcgat agccatcgtc
     2581 ctggagcagg gcaacggttt tgtatagcag gttcatattt tgccaaacac caccactcag
     2641 tgccacatcc tgtaagccga actgtcggga tatttctcgc acaacctcgt gtaccaagtt
     2701 ggccacagca ttgtgaaaac gggccgatat cagatcaatg ggttggctgg tgcgcagatc
     2761 ctcaaccata gcaataaaga gactagctgg gtttgcaata atcaatggac ctgatgtatt
     2821 gtcatcctgt ttcagatatt caaatgagta tgccccctgc tcgtgatggt cagctttcgt
     2881 ctcaaattcg aaggcagcct gcgcttcgta at
//
LOCUS       DBNHOH010000184         6176 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1292668, whole genome shotgun sequence.
ACCESSION   DBNHOH010000184 DBNHOH010000000
VERSION     DBNHOH010000184.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6176)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6176)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6176
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1292668"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="AC16V7_03310"
     CDS             <1..551
                     /locus_tag="AC16V7_03310"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HIR6256179.1"
                     /translation="QIEDLPDRLKVAAIGPKTAAALQKVGVVPDFVPDEYVAEAILPG
                     LGELQDLRVLLLRADLARAALPQAIEHAGGVAHEIAVYNTLPAQPDPQELRDLQLGVD
                     IVTFTSSSTVRNFVSLARQAGLDPQVLPGEPLIACIGPITAQTALEQGLNVDLVASEY
                     TTQGLIQALLEFPSLQITPGAS"
     gene            590..1654
                     /gene="hemB"
                     /locus_tag="AC16V7_03315"
     CDS             590..1654
                     /gene="hemB"
                     /locus_tag="AC16V7_03315"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HIR6256180.1"
                     /translation="MIVDTPSPTQTYTRPTARPRRLRLTSALRGMVRETELNPRDFIY
                     PLFIRPGWGIQNEIKSMPGVYQWSVDRLPAEIESIAKLGIPAVILFGIPSMKDPVGEE
                     NFAPNGIVQEAIRAIKQTVPEMVVITDLCLCEYTDHGHCGVLNVGDELPHPHQPEGYV
                     LNDPTLDVLAQAAVSHAEAGADIVAPSGMIDGMVAAIREALDAARFEHIPILSYAVKY
                     ASAFYGPFRDAAESPPKFGDRKSHQMDPANAREALRETALDVAEGADMLMVKPALPYL
                     DIIHRVREAFPELPLAAYNVSGEYSMIKAAAANGWLNEQLAVMEGLTAIKRAGADLIL
                     TYHAKDAARWLQNMNGSHYG"
     gene            1722..2801
                     /locus_tag="AC16V7_03320"
     CDS             1722..2801
                     /locus_tag="AC16V7_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04053 family radical SAM/SPASM
                     domain-containing protein"
                     /protein_id="HIR6256181.1"
                     /translation="MVHADFDLAPFTIAWEVTRACAFACKHCRADAQHRPDPKELTTD
                     EALHLIDRLADFGSPILIFTGGDPMMRRDLFDLIAHATQRGLRCSLTPTATALPTIDR
                     LTKAKEAGIRRIALSLDAPNPEVHDEFRQVKGSWLRTMKILKNAQSIGLSVQVNTTVS
                     RYNANLLPDMVPFIQEVGAVQWSVFFLVPTGRAQSEWMISPDEHERVFNWLFDLSQNA
                     PFDIKATAAPMYRRVAIQRKKAETIGDAQVTFQGAGFQYADGLNRPTKGVNDGNGFLF
                     ISHLGEIMPSGFLPISAGNVRSDNVVDVYRHHPLFKALRDPAQIKGKCGICDFQDVCG
                     GQRGRAYGLTGDYLETDPACAYEPN"
     gene            2788..3843
                     /gene="hemE"
                     /locus_tag="AC16V7_03325"
     CDS             2788..3843
                     /gene="hemE"
                     /locus_tag="AC16V7_03325"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HIR6256182.1"
                     /translation="MNQTEPQSRFLRACQRLPVDTTPVWFMRQAGRYMPEYRAIREKY
                     SLLEIVQHPEIAVEVTLQPVHALGVDAAILFADILLPIVPMGLHLEFAKGEGPVISNP
                     VRTAADITRLRPIDADTDLGHVMEAIRILRGELPASVPLIGFAGAPFTLASYMIEGGS
                     SRDYRLAKTLMQSEPGLWAEFMDKLATSLENYLLAQVRAGAQAVQLFDSWVGALSPQD
                     YADFVLPYSGRVLKTLEATGVPVIHFGTGTTTLLSLMKQAGGTVIGLDWRIPLDQGWA
                     MLGDGVAIQGNLDPTDLFAPLPVLKRRVEDVLRRAAGRAGHIFNLGHGILQGTPVDHV
                     RAAVEFVHEYHSVQETA"
     gene            3843..5264
                     /gene="hemG"
                     /locus_tag="AC16V7_03330"
     CDS             3843..5264
                     /gene="hemG"
                     /locus_tag="AC16V7_03330"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HIR6256183.1"
                     /translation="MRAVVIGGGIAGLSAAYYLVKMAQQAETALELTVLEREPRWGGK
                     IITEQADGFVIEGGPDTFLATKPWGVALCRELGLESRLHGTNPKQKNTYVLNQSRLQP
                     LPDGLAMMVPTHFGSMARTRLLSLPAKARMGLDFLLPPRNGDQDESLGAFVSRRLGRP
                     AYENLIEPLMSGIYAGDGDRLSLQATFPYLRDMERNHGGLIRGALAMRQKMAKNGGRT
                     NSSRSVFLTPSNGLTEIVDGLVERLAAERVDLRNGISVQAIQKTPGNYEVLLDDNRSL
                     SVDGIILATPAFVSGDLLEGLAPELSAQLRLIRYVTTATISLAYPVQSLPRPLDGYGY
                     VIPRREGRRALACTWTSTKFPHRAPDGYALVRVFIGRAGQEEQIAWDEPSLLQIAQEE
                     LKLTLGITAEPTLARVFIWEQAMPQYELGHPARLKEIDTAIERLPGLALAGNGYRGIG
                     IPDCIHSGEIAAQKILSNFQRSS"
     gene            5261..>6176
                     /gene="hemL"
                     /locus_tag="AC16V7_03335"
     CDS             5261..>6176
                     /gene="hemL"
                     /locus_tag="AC16V7_03335"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016739165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HIR6256184.1"
                     /translation="MNIERSKKLFQEANAILPGGVDSPVRAFRAVGGQPLFIERGDGA
                     YLFDVDGNRYIDYVLSWGPLILGHAHPEVVNSLKEAVQRGTSYGAPSPLEVELAQLIQ
                     SFMPNMEMLRFVNSGTEATMSALRLGRAFTRRDKIIKFEGCYHGHADMLLVQAGSGVA
                     TLGLPDSPGVPVGTAQDTLVARYNDLDSVEALFQRYPDEIAAVIIEPVAGNMGVVPPA
                     DGFLSGLRQLTQQYGALLIFDEVMTGFRVHPGGAQALYGIEPDLSTLGKVIGGGLPVG
                     AYGGRQEIMSLIAPSGPVYQAGTLSGNPL"
BASE COUNT         1324 a         1849 c         1709 g         1294 t
ORIGIN      
        1 tacaaattga agatctacct gatcgcttga aggtcgctgc gatcggcccc aaaaccgccg
       61 cagcgctcca aaaagtggga gtggtgcccg attttgtccc agatgaatat gtggctgaag
      121 cgatcctgcc aggcttggga gaattgcagg acctgcgagt attgctcttg cgagccgacc
      181 tggcacgcgc agccctgccg caagccattg agcacgccgg gggggtggca cacgagatcg
      241 ctgtctacaa caccctgcca gcccagcccg acccgcagga gctgcgcgac ttgcagctgg
      301 gtgtggacat tgtgaccttc accagctcct ccaccgtgcg gaattttgtc agcctggctc
      361 gccaggccgg gctcgatccc caggtactac ccggcgagcc actcatcgca tgcatcgggc
      421 cgatcacagc ccagaccgcc ctagagcaag gattaaatgt agatctggtc gccagtgagt
      481 acacaaccca gggtttgatc caggcattat tagaattccc ctctctacaa ataacaccag
      541 gtgcatcatg atcaattgga aaacatctca acaaaaacag gtaaaaccga tgattgtaga
      601 cactccatca cctacccaaa cctacacccg ccccaccgcc cggccacgcc gactgcgcct
      661 cacttctgcc ttgcgaggca tggtgcgcga gactgagcta aacccgagag attttatcta
      721 cccgttgttc attcgacccg ggtggggaat ccaaaacgag atcaaatcca tgcccggcgt
      781 gtaccagtgg tcagtcgacc gtttgcctgc agaaatagag tcaatcgcta aactgggcat
      841 accagctgtc atcttgtttg gcatcccgtc tatgaaggac ccggttggcg aggagaactt
      901 tgccccaaat gggatcgtcc aggaagccat ccgcgctatc aagcaaacgg tacctgagat
      961 ggtggtaatc accgatcttt gcctatgcga atataccgac catggacatt gcggtgtgct
     1021 caacgtgggc gatgagctgc cccaccccca ccagccagag ggctacgtgt tgaacgaccc
     1081 aaccctggat gtcttagccc aagccgccgt ctcgcacgcc gaggctggcg cggatatcgt
     1141 cgccccgtcg gggatgatag acggcatggt ggcagctatc cgagaagccc tggatgcagc
     1201 tcgcttcgag catatcccca tcctatctta tgctgtcaag tatgcctcag ctttctatgg
     1261 tccgttccgt gatgcggctg aatcgccacc aaaattcggt gaccgcaaat ctcaccagat
     1321 ggatcctgca aatgcgcgcg aagccctgcg cgaaactgcg ttggatgtcg ctgaaggagc
     1381 cgacatgctg atggtcaagc cagccttacc ttacctggat atcatccacc gcgtgcggga
     1441 ggctttccca gagttgcctt tggcagccta caatgtcagc ggcgagtaca gcatgatcaa
     1501 ggcagccgca gccaacggct ggctgaacga gcagttggct gtcatggaag gccttacagc
     1561 catcaagcgc gccggtgccg acctgatcct tacttaccac gccaaagatg cggctcgctg
     1621 gctacaaaac atgaatggat cgcactatgg ataaattttc cgctccccac ccttcactag
     1681 cccctgatgc agccgctgag cgacaatacc acgatcagcg catggtacac gccgatttcg
     1741 atctggctcc cttcaccatc gcctgggaag tgacccgcgc ttgcgctttt gcctgcaagc
     1801 actgccgcgc cgacgcccaa catcgccctg atccaaaaga gctaaccacc gacgaggcgc
     1861 tacacttgat tgatcggctg gctgattttg gtagcccgat attgatcttc actggtggcg
     1921 atcccatgat gcgccgcgac ctgtttgatt tgatcgctca tgccacccag agaggcctgc
     1981 gttgctcgct gacccccact gctaccgcct tgccaaccat agatcgcctt accaaggcta
     2041 aggaggccgg catacggcgc atagccctga gcctggacgc tcctaaccca gaagtgcacg
     2101 atgagttccg ccaggtgaaa ggctcctggc tgcgcaccat gaaaatactg aagaacgcgc
     2161 aatccatcgg gctgagtgtg caggtaaaca caaccgtgtc acgctacaat gcaaacctgt
     2221 tacccgacat ggttccattt attcaagagg tgggcgcagt gcagtggtca gtattcttcc
     2281 tcgtacccac agggcgcgcg caatcagaat ggatgatctc cccggatgag cacgagcgtg
     2341 tcttcaactg gctctttgac ttatcccaaa acgctccatt cgatatcaag gccacagcag
     2401 cgcccatgta tcgccgtgtc gccatccaac gcaagaaagc cgagactatc ggagatgctc
     2461 aggtgacatt tcagggtgct ggcttccaat acgctgatgg tttgaaccgg cccaccaagg
     2521 gcgtcaacga cggcaacggt ttcctgttca tctctcacct gggtgagatt atgccatctg
     2581 gcttcctgcc tatctcggcg ggcaatgtac gcagcgataa tgtggtcgat gtataccgcc
     2641 accatccact gtttaaagcc ctgcgcgatc cggcgcaaat caagggtaag tgcgggattt
     2701 gtgacttcca ggacgtctgc ggtggtcaac gtgggcgtgc ctacggtttg accggagatt
     2761 atctagaaac ggaccctgct tgtgcctatg aaccaaactg aaccccaatc ccgcttttta
     2821 cgcgcctgcc agcgcctgcc cgtagatacc acccccgtgt ggtttatgcg ccaggcaggt
     2881 cgttatatgc cagaataccg cgccatccgt gagaaatact cactcctgga gattgtgcaa
     2941 catccggaga tcgccgttga ggtgacgctg cagccggtgc atgctttggg cgtagatgct
     3001 gccatcctat tcgcagacat cctgctgccg atcgtcccca tgggcttaca cctggaattt
     3061 gctaagggtg agggaccagt gatctccaat ccagtgcgaa cagctgccga tatcacccgc
     3121 ctgcgcccga tcgatgccga tactgacctg ggtcatgtga tggaagctat ccgcatcctg
     3181 cgtggcgagc tacccgccag cgtacccctg atcggttttg ctggcgctcc attcacactg
     3241 gcttcctaca tgatcgaggg cggctcttcg cgcgattacc gcctggccaa gaccctgatg
     3301 caatctgagc caggcttgtg ggcagaattt atggacaagc tggcgacctc tcttgagaat
     3361 taccttctcg cacaggtgcg cgctggagcc caggcggtac agcttttcga ctcgtgggtt
     3421 ggcgcgctca gcccgcagga ctatgcggat ttcgtcctgc cttattccgg gcgcgtcctc
     3481 aaaactcttg aagcgactgg cgtgccagtt atccactttg gaacaggcac cacaacctta
     3541 ttgtcgctga tgaaacaggc tggcggcacg gtaatcggcc tggactggcg catccctctt
     3601 gaccagggct gggcaatgct gggcgatggg gttgctatcc agggcaacct ggaccccaca
     3661 gacctttttg cccccctgcc agtcttgaaa cgccgcgtgg aggatgtcct gcggcgtgct
     3721 gcaggccggg caggtcacat cttcaacttg ggtcacggca tcctgcaagg cacacctgtg
     3781 gatcatgtcc gcgctgccgt agaattcgtc cacgaatatc actcggtgca ggagacggca
     3841 taatgcgtgc tgttgtaatc ggagggggca tcgccggtct ctcggctgcc tattacctgg
     3901 taaagatggc tcagcaagcg gagaccgcgc ttgaattaac cgtcttagaa cgcgagccgc
     3961 gctggggcgg caagatcatc accgagcagg cggatggatt cgtcatcgag ggtggtccag
     4021 atacttttct agccactaaa ccctgggggg tggcattgtg ccgcgagctg ggattagaat
     4081 cacggctgca cggcaccaat ccgaagcaaa agaataccta cgtgctgaac cagagtcgcc
     4141 tgcagcctct accagatgga ctggcgatga tggtgcccac tcattttgga tccatggcgc
     4201 gcacccgcct gctatctttg cctgccaagg ctcggatggg gttggacttc ctgctccccc
     4261 cacgaaatgg tgaccaagac gaaagtctgg gcgcatttgt ctctcgccgt ctgggacgcc
     4321 ctgcctatga aaatctcatt gagccgctaa tgagcggtat ctatgctgga gatggcgacc
     4381 gcctcagctt acaagccact ttcccctacc tgagagacat ggagagaaat cacggcgggc
     4441 tgatccgtgg cgccctggcg atgcgccaga agatggcaaa aaatggcggc agaacaaaca
     4501 gctctcgcag cgtctttctc accccatcaa atggattaac cgagattgta gacggtctcg
     4561 ttgagcgatt agccgccgaa cgagttgact tgaggaacgg aatcagcgtg caggctatcc
     4621 aaaagacacc tggaaattac gaagtgttat tggacgacaa tcgatcgttg tctgtggatg
     4681 gtatcatcct ggcgacgcct gcttttgtca gcggcgacct gctggagggg ttagcccctg
     4741 agttaagtgc ccaattgcgc ctcattcgct acgtaaccac cgccacgatc tcgctggcat
     4801 atcccgtgca aagcttgcca cgcccgctgg atggctatgg ctatgtcatc ccccgccgcg
     4861 aggggcgacg cgcgctggcc tgtacctgga cttccacaaa gttccctcac cgcgccccag
     4921 atgggtatgc actcgtacgg gtcttcatcg ggcgcgcagg gcaggaagaa cagattgcct
     4981 gggatgaacc cagcctgctc cagatcgcac aagaggagct caaacttacc ctgggtatta
     5041 ccgctgaacc aaccctggcg cgtgtcttca tttgggagca agccatgccc caatacgagc
     5101 tgggacatcc agcccggcta aaggaaatcg acacagccat cgagcgcttg cccggtctgg
     5161 ccctggcggg caatggctac cgcggcattg gcatcccaga ctgcatccat tctggagaga
     5221 ttgcggcaca gaaaatatta tccaactttc aaaggtcatc atgaatatcg agcgatcaaa
     5281 aaagctattt caggaagcga atgctatcct gcctggcggt gtggattcac ctgtacgcgc
     5341 atttcgtgca gtgggtgggc aaccgctttt catcgagcga ggtgacgggg catacctgtt
     5401 cgacgtggac ggcaaccgct atattgacta tgtgctctcc tggggaccgc ttatcctggg
     5461 acatgcccac ccggaagtgg taaattccct gaaggaggca gtgcagcgtg gcacatccta
     5521 tggggcgccc agcccgctgg aggtggagct ggcgcaatta attcagtcct tcatgcccaa
     5581 tatggaaatg ctgcgcttcg tcaactcggg aacagaagcc accatgagcg ccctgcgcct
     5641 ggggcgcgcc ttcacccgcc gagataagat catcaaattt gagggctgct accacggtca
     5701 cgccgacatg ctgctggtgc aagccggctc aggggttgct accctgggct taccggactc
     5761 acccggcgtg cctgtcggga ccgctcagga taccctggtg gcgcgctata acgatctcga
     5821 ctccgtcgaa gccctctttc agcgctatcc tgacgagatc gctgcggtga tcatcgagcc
     5881 agtggcaggc aatatgggcg tcgtgccccc tgcagatgga ttcctctccg gtctgcgcca
     5941 gctgacacag caatatggcg cactgctgat cttcgatgag gtgatgaccg ggttccgcgt
     6001 ccaccccggt ggggcacaag ccctgtatgg gatcgagccc gacctgagca cgctgggcaa
     6061 ggtgattggc ggcgggctgc ctgtgggcgc ctatggcggg cggcaggaga tcatgtcgct
     6121 gatcgccccc tctggaccag tgtaccaggc tggtacgctc tctggaaatc cactgg
//
LOCUS       DBNHOH010000185         2246 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1100704, whole genome shotgun sequence.
ACCESSION   DBNHOH010000185 DBNHOH010000000
VERSION     DBNHOH010000185.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1100704"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="AC16V7_03340"
     CDS             complement(<1..211)
                     /locus_tag="AC16V7_03340"
                     /inference="COORDINATES: protein motif:HMM:NF018012.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR6256185.1"
                     /translation="MIKLNVLTENDIEAIHESSLRILAETGIVLTHPQGREILAAAGA
                     TVESDRVLLPPELVEEAIARCSNEVR"
     gene            complement(218..2017)
                     /locus_tag="AC16V7_03345"
     CDS             complement(218..2017)
                     /locus_tag="AC16V7_03345"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017280.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR6256186.1"
                     /translation="MPNSPIIDLEKLMRAPGVDEEHPFDISPDGQKVAFSWNKSGQWE
                     VYELPLDGSAPPRQITSGEGAKFSPRYSPGGERLAYMVDFDGGENFRLFIHDLTSGAQ
                     VNLTPNEPAALQSNFAWSPDGTQIAILSNRSGSFKTYIMPAAGGASRLVLDTPYPDWD
                     VRWSPEGSWLLVIADTEGSDQGIFIVPASGGEVIQLSDENGPLSAKQGRWSPDGKRIA
                     FASNSRGFYDIGIYTLATGEINWVTQGYGDKISPSWSPDGKRLVYIYNADGVTHLAVH
                     SLGKPTPETYQVEPGLHLLPQFTPRGEALVFIFNNPRCPDDLWHLTLSDANRRQLTSS
                     LPPDLQEAAFVMPSVERIPGLDGVPVPALLFQPPPAKDLSPAVVLVHGGPNWHLDVMW
                     YPLVQHMTSRGWVVIAPNYRGSTGYGREWQYASRFDQGGVDTRDVVATAQYLSQQGLA
                     DPKRIAVTGTSHGGYLTMMALTQYPQVWAAGSAVVPFLNWFTSHANSRDDLQHWDIEN
                     LGDPEKDYDLWHERSPFFFLDRVNASVQLICGANDVRCPASESIAARDALEALGKPVD
                     FHLYPDEGHSFLKTENVIDAARRRVEFLARVLE"
BASE COUNT          473 a          610 c          659 g          504 t
ORIGIN      
        1 agcgcacttc gttcgagcag cgggctatgg cttcttcgac aagctcgggt gggagcagga
       61 cacggtcgct ttctaccgtg gccccagccg cagccaatat ctcgcgccct tgcgggtgtg
      121 tcagcacgat gccggtctcg gcaaggatgc gcaggctgga ttcgtggatc gcctcgatat
      181 cgttttcggt aagcacattt agtttgatca taggtttcta ttccaatact ctggctaaaa
      241 attctaccct gcgccgggcc gcgtcgatga cgttctctgt cttgaggaag ctgtgccctt
      301 cgtcgggata gagatggaaa tctacaggct tccccagcgc ttccagggca tcacgggcgg
      361 cgatggattc gctggctggg cagcgcacat cgttggcgcc gcagatcaac tgcacggagg
      421 cgttaacccg gtctaggaag aagaagggcg atcgctcgtg ccataagtca taatctttct
      481 caggatcgcc gaggttctct atatcccaat gttgcaggtc gtcgcgagaa ttggcgtggg
      541 aggtaaacca gttcagaaaa ggcaccactg cggaaccggc ggcccaaacc tgcgggtatt
      601 gggtgagagc catcatggtc aggtaaccac cgtggctggt tccggtgacg gcgatgcgct
      661 tagggtcagc tagcccttgt tggcttaggt attgcgcggt agccaccacg tcgcgcgtat
      721 ccaccccgcc ctggtcgaag cggctggcat actgccattc tcgcccgtag ccagtggagc
      781 cacggtagtt tggggcaatg acaacccacc cccggctggt catgtgttgg acgaggggat
      841 accacatcac gtccaggtgc cagttgggcc cgccgtgtac cagaaccacc gctggcgata
      901 aatcctttgc ggggggtggc tggaataata atgcgggtac agggacgccg tccaggccgg
      961 gaattcgctc gacagacggc atgacaaaag cagcctcctg cagatcgggc ggtagcgagc
     1021 ttgtaagctg tcggcggttc gcgtcgctaa gcgtcagatg ccacaggtcg tcggggcagc
     1081 gcgggttatt gaagatgaaa actagtgcct cacccctcgg tgtaaactgc ggcagtaaat
     1141 gcagaccggg ctccacctga taagtttcag gggtgggttt gcccagcgag tgcaccgcca
     1201 ggtgggtcac cccatctgcg ttatagatat ataccagccg tttgccgtca ggcgaccagc
     1261 ttggcgaaat cttatcgcca taaccttgcg tcacccaatt aatctctccc gtggcgagtg
     1321 tgtagatgcc gatatcataa aaaccacgcg agttcgaggc gaaagctatg cgttttccat
     1381 ctggtgacca gcgcccttgt ttggcgctca atggcccgtt ttcatccgat aattggatca
     1441 cctcgccacc actggcaggg acaatgaaga ttccttggtc tgatccttcg gtgtcagcga
     1501 tcaccagcag ccagctacct tccggggacc agcgcacatc ccaatctggg tagggggtgt
     1561 ctagcaccag gcggcttgcc ccaccggcag ccggcatgat ataggttttg aagctgcccg
     1621 agcggttgga taagatggcg atctgggttc catccggcga ccaggcgaaa ttagattgca
     1681 gggcggcggg ctcgtttggg gtcaagttca cctgcgctcc gctggtgagg tcatggatga
     1741 aaagtcggaa attctcacca ccgtcgaaat ccaccatgta ggccaaacgc tccccaccgg
     1801 gagaatagcg tggcgagaat ttcgcaccct ctccgctggt gatctggcga ggcggtgcgc
     1861 taccgtccag gggaagctcg taaacctccc actgcccgct tttgttccat gaaaaggcta
     1921 ccttctgacc atctggagag atgtcgaaag gatgctcttc atccaccccg ggggcgcgca
     1981 tgagtttttc gagatcaatg atggggctat tgggcatcgg atcttgttct cacttgtgag
     2041 gaattatttt aacctacacc cttgttttgc agggtggatt gtccgactca caaaggaggg
     2101 aaattgcgta ggaagaaatc aaaataacga tccatcgtat tgatggatgt attaaccaga
     2161 ttgactcatc actttataca ggcggtttag tccctcgttc aggtaatccg ctggcaagcc
     2221 atagcttatc cgcaggaagt ggtcca
//
LOCUS       DBNHOH010000186         2705 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_266969, whole genome shotgun sequence.
ACCESSION   DBNHOH010000186 DBNHOH010000000
VERSION     DBNHOH010000186.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_266969"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="AC16V7_03350"
     CDS             complement(<1..316)
                     /locus_tag="AC16V7_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256187.1"
                     /translation="MDIYLATERAFHLVPQFTQEVARDRLEQKKTSLVAGTVGSMISR
                     PRPDEIQLVSVESRLEAFWLIDVFVRTTYDNNRTYTIPVSGPEVKQVTALGQDLQVTS
                     SPK"
     gene            675..1625
                     /locus_tag="AC16V7_03355"
     CDS             675..1625
                     /locus_tag="AC16V7_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256188.1"
                     /translation="MKRIPSFLSILILLALGACTLRMPGVEAPSAIVSTPETLTGEVV
                     APTEVIAPAQQAAPTDTAALPTVAMPAAALPTPYPAPETQAPPPPTSEPTATQPAPTA
                     TPTPVITIDPNTAYGEPDYVNRMQFPNLSEWAPPETGELPNNRNIRLQFKDGELYVTG
                     KRINFSTWWFSYHNLSDAYIEMTFETEDCSGEDAYGLIFRGPPHLAGESYGYAVSFTC
                     SGNLWVLRLDGVNPWEAETLIDMEDIYAIRTGKDEENVIGVRAEGERFVIYANGNQIA
                     EVEDDHFETGRGGVFVRAGRPGAYTYRVTNFAYWILGEDE"
     gene            complement(1787..2512)
                     /locus_tag="AC16V7_03360"
     CDS             complement(1787..2512)
                     /locus_tag="AC16V7_03360"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR6256189.1"
                     /translation="MSSNYSPVVDDVISVIRHLGQQSAALELTKSFRGIIFKQDVSIL
                     EVSPDDATFRASNIEMCAATETEVYLHNQLFPKPVMAQLKSLNIRKGMLVLSDFAYAD
                     MEWKKRQHERVQPKCPIYVTLHWKGKALRACMENISVDGLGLLAYKLFEKGMNIRPGS
                     NIRLDFQLSPGQKFSALRGRIIYINTIDRYSTTMGIRLFPKVKEVRVLEKYVARRKQE
                     ILEELNQEYSELSKPRGVDSLYF"
BASE COUNT          684 a          688 c          688 g          645 t
ORIGIN      
        1 ccttggggct ggaggtaacc tgcaagtctt gccccagcgc agtaacctgt ttgacttccg
       61 ggccgcttac cgggatggtg taagtgcggt tattatcgta cgttgtgcgc acaaagacgt
      121 caatcagcca aaaagcttcc agccggctct cgacagacac caattgaatc tcgtcgggcc
      181 tgggacggga gatcatcgac cccacggtcc cagcgacaag gctggttttc ttctgttcta
      241 ggcgatctcg tgccacctcc tgcgtgaatt gagggaccag atgaaaagct cgttcagtag
      301 cgagatagat gtccatatgg gatcctgccc tgtattttgg attctcggcg cggctgccca
      361 gcggaatttg tctatgaaaa gacgatctgg gcagcgctat aacgataatt atgacataaa
      421 accagcagat cgtgtaacgg ctaatttatc tgcccggagc ccttgtgtgc gggtccaagt
      481 ccacaaaaca gggatttagc ctcgggcttg tgatactgta aggtaatagc ttcgaccaca
      541 tgttataatc tttttgttct cctgcgggca gtaagaccta tatcaatcat caattgactt
      601 atctccattc caggtagcga caggatgtcg atgccaaaat aaagctaaaa gaaactatag
      661 attgggaaaa cctcatgaag agaattccat cctttctatc catcctgatc ctgcttgcct
      721 tgggcgcgtg cacactgagg atgcccgggg tagaagcacc tagcgcgatt gtctccacgc
      781 cggaaactct aaccggagaa gtcgttgcgc ctaccgaagt gatcgccccc gcccaacagg
      841 cagcccccac cgataccgca gcgctgccca ccgtggctat gccggcggca gcgctgccca
      901 cgccataccc agcgcccgaa acacaagccc ctccgccacc caccagcgag cccacagcca
      961 cccaaccggc gcctacggca acccccacgc cagtcattac cattgacccg aacacggcat
     1021 atggagagcc agattacgtg aaccggatgc aattccccaa tctgagtgag tgggcgccac
     1081 ccgagacggg cgagctaccc aacaaccgca acatccgctt gcagtttaaa gatggtgagc
     1141 tgtacgtcac gggcaagcgc ataaatttca gcacctggtg gttcagttat cacaacttga
     1201 gtgatgctta tatcgaaatg accttcgaga ccgaagattg ctctggcgag gatgcatatg
     1261 ggctcatctt tcgcggccca ccacacctgg caggggaatc gtacggttac gccgtatcgt
     1321 tcacttgtag cggaaattta tgggtgttgc gcctagatgg ggtaaatccc tgggaggctg
     1381 agaccctaat tgacatggaa gatatttatg cgatccgcac cgggaaggat gaagaaaatg
     1441 tgattggggt gcgcgccgag ggcgagcgtt tcgtcatcta tgctaatggc aatcagattg
     1501 cagaggtgga ggacgatcat ttcgagacgg ggcggggggg cgtgttcgtg cgggcaggta
     1561 ggcctggggc ttacacctac cgcgtcacga attttgcata ttggatcctg ggggaggatg
     1621 aatagatcaa tagaccggta gtatcccgag ttggcctaca gggtatcgag ggaaagccgg
     1681 ccgcggcagt taccggagtg agccaatccg gcagggatta catcgagtat agatagtcgg
     1741 gattaattgg gcgacgcttg tctatgctaa aaaggtccgc aagagattag aaataaaggc
     1801 tatccactcc cctgggcttg ctcaattctg aatattcttg gttgagctcc tcgagaatct
     1861 cctgcttgcg gcgagcaaca tatttttcca aaacacgcac ttctttaacc tttggaaaca
     1921 accggatacc cattgtggtt gagtatcgat ctattgtatt tatgtagatg atcctgcccc
     1981 tcaaagctga gaatttttgt ccaggagaga gttggaaatc caatcgaata tttgagccgg
     2041 ggcggatatt catccccttt tcgaaaagct tataagccaa aagtcccaat ccatcgaccg
     2101 agatattctc catgcaagcg cgaagggctt tccctttcca atgcaaggta acataaatgg
     2161 gacattttgg ctggaccctc tcgtgctggc gttttttcca ctccatatcg gcataagcga
     2221 agtctgagag caccaacata cctttgcgaa tattcagact cttgagctgc gccatcactg
     2281 gtttgggaaa taactggtta tgcaggtaaa cttccgtctc cgtggcagcg cacatctcga
     2341 tgttggatgc ccgaaaggta gcatcatctg ggctcacctc aaggatgctg acatcctgct
     2401 taaagatgat cccccggaat gattttgtca attccaaggc ggcgctttgt tgaccaagat
     2461 gcctgataac ggatattaca tcgtcaacga caggcgagta atttgaggac acggataaat
     2521 cctcctttgg cccccgtaag ccaatcagtt tttagagcga tggatgtaat ccaccctcaa
     2581 ttgagatggt ccatgctcac aagaaagtgt acacaggttc ctttataaat acatcataag
     2641 gcaggattga ggttttcttc ttaacaaaat tgaaattatt tagtcatcaa tctaagtaga
     2701 ttagc
//
LOCUS       DBNHOH010000187         2273 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_651948, whole genome shotgun sequence.
ACCESSION   DBNHOH010000187 DBNHOH010000000
VERSION     DBNHOH010000187.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_651948"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..1520
                     /locus_tag="AC16V7_03365"
     CDS             192..1520
                     /locus_tag="AC16V7_03365"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012457.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HIR6256190.1"
                     /translation="MAQATFHFPRGFLWGTATAAYQVEGKNIHSNWWAWEQEPGRILN
                     GHKSGPACDWWGGRWREDFDRAADAGQNAHRFSVEWSRIQPTPDRWDENALDRYREML
                     RGLKERGMTPMVTLHHYTEPNWITELGGWENEAIGGHFEKYVHKVVEALKEYVNLWIT
                     LNEPNALITRSYILGDFPPGRMNIRTAFRAGVNLVQAHAVSYHAIHKQQPEARVGIAQ
                     MYRSFQPARHWFPLDRWVTRAQSRSFNDMFPKALTDGVVRTPFGKTLIPRAKQTQDFL
                     GINYYTRDQVMFDLMKPSELFGRRYYRPEAELSESGFIAHEPDGIYEALTWGNQFNVP
                     MIITENGVEDASDGLRPRYLVEHLHQVWRAVNLNLPVRGYFHWTLVDNFEWAAGWTMR
                     FGLWELDPETQTRRKRPSADLYAEICRENGISSEMVARYAPQIFEKMFPN"
     gene            1549..>2273
                     /locus_tag="AC16V7_03370"
     CDS             1549..>2273
                     /locus_tag="AC16V7_03370"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6256191.1"
                     /translation="MISPDSLHPPGIFRVVCRPVLPKDSPDVLELTRWIWGGHDYIHL
                     VWADWLKDTEGLLAVAEYGGHVVGLYKLTRLSPSEWWLEGLRVHPDYEGRGIASRLHD
                     YLMAYWERHRGGTIRLGTSSARKPVHHLSLRSGFRKIMGLSFYAAENKPGPIGRVAPM
                     QPGEENEALNFALHNPTPTFPNGLMDTGWKWVAPSVDRITQGITSGHAWWWNDDGGRQ
                     AIILARRDVEQGVEDLMVQLLVCP"
BASE COUNT          540 a          588 c          643 g          502 t
ORIGIN      
        1 atgcccccac caaaacaccc caaacactct ttttacggga ttgcgcatgc ccctcagggc
       61 atatgcaatt gccctgttga gaatctacca agcagagtaa accgataaga ttgccttgcc
      121 cgcgaccggg tttgtcaaat gcggcgggta ggaatacaat tgcataaaac ctttaggcat
      181 tggagtagat aatggcacaa gcgacctttc atttccctcg cgggtttcta tggggtacag
      241 ccacagcagc ttatcaagta gaaggtaaga atatccattc caattggtgg gcctgggagc
      301 aggaaccagg gcgcattctc aatgggcata aatctggtcc cgcctgcgac tggtggggcg
      361 ggcgctggcg tgaagatttt gaccgtgctg ccgatgccgg acaaaatgcc catcgctttt
      421 ccgtcgaatg gagccgcatc caacccacac cagaccgctg ggatgagaat gccttggatc
      481 gctatcgcga gatgttgcgc gggctgaaag agcggggcat gacccccatg gttacactgc
      541 accactacac tgagccgaat tggattaccg aattaggcgg ttgggagaat gaagccattg
      601 gtgggcattt tgaaaaatat gtccacaaag tggtggaagc cttaaaagag tacgttaact
      661 tgtggatcac gctcaatgag ccaaatgcgt tgatcacgcg cagctatatt ttgggtgatt
      721 tcccgccagg taggatgaac atccgtaccg ccttcagggc tggggtgaat ctggtgcaag
      781 cccacgccgt gtcctaccac gccatccaca aacaacagcc tgaggcgcgg gttggcattg
      841 cccaaatgta ccgcagcttc cagccagcgc gccactggtt cccgctggat cgctgggtta
      901 caagagccca atccagatca tttaatgata tgtttcctaa agctttgaca gatggcgtag
      961 tgcgcacacc atttgggaag accctaattc ctcgtgctaa acaaacacaa gatttcctcg
     1021 gcatcaacta ttacacgcgt gaccaggtga tgtttgatct catgaaaccc agcgaattgt
     1081 tcggtcggcg ctattaccgc ccagaggcgg agttgagcga aagcggtttc atcgcacacg
     1141 agccggatgg gatatacgaa gcgctcacgt ggggcaatca attcaatgta cccatgatca
     1201 tcacggagaa cggggtagaa gatgccagcg atggcttgcg cccgcgttat ctggttgaac
     1261 atttacacca ggtgtggaga gcggttaatc tcaacctgcc agtgcgcggc tatttccatt
     1321 ggacactggt ggataatttt gaatgggcag caggctggac aatgcgcttc ggactctggg
     1381 agctagatcc ggagacacag acgcggcgta agcgccccag cgcagacttg tacgctgaaa
     1441 tctgccgcga gaatggcatt tcgtctgaaa tggtggcgcg ctacgccccc caaatctttg
     1501 agaagatgtt tccaaactga ttgttgagtg ctgacttgct gagtgggtat gatatcgcct
     1561 gattctttgc accctccagg aatcttccgg gtggtgtgtc gcccggtttt gcccaaagac
     1621 tctccagatg tgctagaact gacccgctgg atttggggtg ggcacgacta catccacctg
     1681 gtttgggcgg attggctcaa ggataccgag ggcttactag cggtggcaga gtacggcggg
     1741 cacgtggttg ggttatacaa gctgacgcgc ctatcaccaa gcgagtggtg gctggagggc
     1801 ctgcgcgtgc atcccgatta tgaagggcgc ggcatcgcct cgcgacttca cgactaccta
     1861 atggcgtact gggagcgcca tcggggtggc accatccgcc tggggacatc ctcagcccgt
     1921 aagccggtac atcacctcag cctgcgcagt ggctttcgaa aaatcatggg gttatcattt
     1981 tacgcagcgg aaaacaagcc cggcccgatt ggacgagtcg ctccgatgca gcccggtgaa
     2041 gaaaatgaag cgctgaactt tgccttgcat aatccaaccc ccactttccc caatgggcta
     2101 atggataccg gttggaaatg ggtagcaccc tcagtggatc gtatcaccca ggggataacc
     2161 tcggggcacg cctggtggtg gaatgatgat ggaggcaggc aagctatcat cttagcgcgc
     2221 agagatgtag aacaaggtgt agaagacctc atggtccaat tattggtctg ccc
//
LOCUS       DBNHOH010000188         2772 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_908755, whole genome shotgun sequence.
ACCESSION   DBNHOH010000188 DBNHOH010000000
VERSION     DBNHOH010000188.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_908755"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="AC16V7_03375"
     CDS             <1..1170
                     /locus_tag="AC16V7_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HIR6256192.1"
                     /translation="SYQWDLNYANPVVFNRMVEEMLFLANQGVEVLRLDAVAFIWKEL
                     GTNCESLPKAHMLIQAFNAAARIAAPALLFKSEAIVHPDEVIQYISPDECQLSYNPLL
                     MALLWEAMATRKVGLLSQALRDRFAIPTHCAWVNYVRCHDDIGWTFSDEDADRLGING
                     YSHRKFLNDFYTGRFPGSFARGLPFQENPKTGDARISGTCASLAGLEKAVREETDVEV
                     ELAIRRILLIHGVILTVGGIPLIYLGDEIGSLNDYSFRDDPGKAADSRWVHRMRVDWE
                     KYARRSNSATLEGRVYQGICRLINLRLSRPVFAGQDMQVLETDNENVLGYLRRHDNSI
                     ALIFANFSEREQTVSANLLRLYGLSYNTQDMFSGAKLPQGDINLKPYDFLALAPQ"
     gene            1470..>2772
                     /locus_tag="AC16V7_03380"
     CDS             1470..>2772
                     /locus_tag="AC16V7_03380"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR6256193.1"
                     /translation="MALLRAYLFRSIWVVTEDGRTLDLGSPTVRSLFAYLLLHRDHPS
                     DRRRLAFLFWPRASEAAARRNLRQYLHRLRRALEPVDPLGGLLIAKHSGVQLDPSAQI
                     WVDVEAFRHNTRPEATLPDLQTAAELYSGDLLEDVYEDWCDETRQELRQSYLNLLDRL
                     SQILQDMGRLDEANAFAQKWIGSEPLDEAGHRRLLSLYALAGDRGRAVSHYQSLKEKL
                     EEELNAEPLPETQILFQAIQRGTYHPDQHLPSTSSVRRTLLPQPTALPLVGRQQELAR
                     LHDALQNARQGRGNFILISGDSGIGKTRLVQEYLSLYPGLLTLQGVCHELESVVPYAS
                     LRQALVEAGNFLPPSMLKTPPSWLVTLGYFIPSLTTQLPYLPSRAPMQEESPRLLEAL
                     MNLTMTINTGTPDQPLHLIIDDLQWCDGPTWDFLLQLSHQAE"
BASE COUNT          655 a          688 c          698 g          731 t
ORIGIN      
        1 tcttaccaat gggacttgaa ttatgcaaat ccagttgttt tcaaccgcat ggttgaggag
       61 atgcttttcc tggcaaatca aggggtagaa gtgctgcgtc tagatgccgt tgccttcatt
      121 tggaaggagt tgggaacgaa ttgcgagagc ttacccaaag cccatatgct gatacaggct
      181 ttcaacgctg cggcgcgtat tgctgctcca gcccttttgt ttaaatcaga agctatcgtt
      241 cacccggatg aagttatcca atatatctca cccgacgaat gccagctatc atataatcct
      301 ttactcatgg ccttgctgtg ggaagccatg gctacccgta aggtgggttt gctgtcccaa
      361 gcgctgcgcg accgcttcgc aattcctaca cactgtgcct gggtcaatta tgtgcgctgt
      421 catgatgaca tagggtggac attttcagac gaagatgctg accgtttggg cataaatggt
      481 tattcccacc gaaaattttt gaacgatttt tataccggac gcttccctgg aagctttgcc
      541 agagggttgc cctttcagga aaatcctaag acaggcgatg cgcgcatttc agggacctgt
      601 gcttcgttag cgggattaga gaaagcggtg cgcgaggaaa ctgatgttga agtcgagctt
      661 gccatccgtc gtatcttgct cattcacgga gtaatcctta ctgtaggtgg tatccctctg
      721 atctacttgg gagacgagat tggcagtttg aatgattaca gcttccgtga cgatcctggc
      781 aaggcagcag atagccgatg ggtgcatcga atgagagtcg actgggaaaa atatgcccga
      841 cgttcaaatt ctgctacgct cgagggacgg gtttaccagg ggatctgccg cttgattaat
      901 ttgcgtttga gtaggcctgt atttgctggt caggatatgc aagtgctcga aacggataac
      961 gagaatgttt taggctattt acgtaggcac gataacagta tcgctctcat ttttgctaac
     1021 ttcagtgagc gtgaacaaac agtttccgcc aaccttcttc gtctctatgg tctgagttac
     1081 aatactcagg atatgttttc aggggcaaag cttccacaag gggatataaa tttgaaacca
     1141 tacgatttcc tcgctctggc tccacaataa gtttgagggc atctgttgcc ccaaatcaga
     1201 taacgtcagt agttgatgaa ttagatcgta catggtggta caggaattca tccgagcaaa
     1261 actcactgcg caaattgggc aatcgcattt gggcgatttc ccaccactga ggagagaaaa
     1321 tgaggtgttt tgatgggggt atgcccccac caaaacaccc caaacactct ttttacggga
     1381 ttgcgcatgc ccctcagggc atatgcaatt gccctgctgc gcaaagatgg tgatatgaac
     1441 cctatcgttt ataatgggaa catatctcca tggcgctctt aagggcttac cttttccgtt
     1501 ctatttgggt tgtaactgag gatgggcgca cgctcgattt gggttcgcct actgtgcgtt
     1561 cattgttcgc gtatcttctg ttacaccgcg accatcccag cgacaggagg cggctggcat
     1621 ttttgttctg gccacgagcc agcgaagccg cagcccgtcg aaacttgcgt caatatcttc
     1681 atcggttgcg gcgtgcactg gagccagttg atccactagg tgggttgtta attgccaagc
     1741 actccggtgt acagctggat ccaagtgccc agatatgggt ggatgtagag gcgtttcggc
     1801 acaatacgcg ccctgaagct acacttcccg atttacaaac cgcagcggag ttgtactctg
     1861 gcgatcttct cgaagatgta tatgaagact ggtgtgacga aacacgccaa gagctgcgtc
     1921 aatcttatct caacttactc gatcgtttaa gccagattct acaggacatg ggacgcctgg
     1981 atgaagctaa cgcctttgcc caaaaatgga ttggttctga gcccctggat gaagctggcc
     2041 atcgcaggct gctatccctc tacgcgttag caggggatag aggccgagct gtgtcccact
     2101 atcagtcatt gaaggagaaa ttagaggaag agctcaacgc tgaaccatta ccggagaccc
     2161 agatactttt ccaggcgatt caacgcggta cttatcatcc ggatcagcat ctcccttcga
     2221 catcttctgt tagacgcaca ctgctacccc agccaactgc gttacccttg gttggccgac
     2281 agcaggagct agcccgactg cacgacgcgc tgcaaaacgc ccggcagggg cgtggaaact
     2341 ttatcttgat cagcggggat tctgggattg gaaagacccg tcttgtccag gaatatctct
     2401 ctctttatcc cggtttgctc acactccagg gtgtttgcca cgagcttgag tcagttgtgc
     2461 cttacgcgtc gctgcggcaa gcccttgttg aggcaggcaa ttttctgccg ccaagtatgc
     2521 tgaagacccc gccaagttgg ttggtcacgc tcggttattt tattccatca ttaaccactc
     2581 agctacctta ccttccatcg cgtgctccca tgcaagagga aagtcctcgc ttgctcgaag
     2641 cgcttatgaa tttgactatg accataaaca ctggcactcc agatcagcca ttacacttga
     2701 tcatagatga tttacagtgg tgtgatggcc ccacctggga tttcctgcta caactttccc
     2761 accaggcaga aa
//
LOCUS       DBNHOH010000189         1999 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1357272, whole genome shotgun sequence.
ACCESSION   DBNHOH010000189 DBNHOH010000000
VERSION     DBNHOH010000189.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1357272"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="AC16V7_03385"
     CDS             complement(<1..591)
                     /locus_tag="AC16V7_03385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6256194.1"
                     /translation="MPDYRICLTPKVSGVGGMVSFRRRLAAGLVQRGVEVGDDLDDGR
                     YDAVLVIGGTRQLAGLWRARRRGARIVQRLNGMNWLHRLRRTGLRHYLRSEYGNWLLS
                     FIRSRLAGRIVYQSEFARRWWEQVYAPTAVPWRVIYNGVDLSAYSPVGPGQPPQDRWR
                     ILLVEGSLMGGYELGLEASVGLVNEISQHLNHTDSLS"
     gene            complement(595..1032)
                     /locus_tag="AC16V7_03390"
     CDS             complement(595..1032)
                     /locus_tag="AC16V7_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256195.1"
                     /translation="MDAYSFLVYARTWPILGRLAYYALKILGVEVPRSVKIGQDFELA
                     HGGFGVVIHPRSSIGDRVKIYPGVGLGRADIHLPMERSRFEGIVIEDDVILSPGCKLL
                     CKDGVLRVGRGTVIGANAVLLQSTGEWEMWAGVPAKLVGKRDR"
     gene            complement(1043..>1999)
                     /locus_tag="AC16V7_03395"
     CDS             complement(1043..>1999)
                     /locus_tag="AC16V7_03395"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HIR6256196.1"
                     /translation="HLRPGNLVVLESTSPPRTTVDVVAPILARSGLNPGVDFYLAYSP
                     ERVLPGQILRELIENARVIGGVNRASAEAGRDLYEVFVRGEIILTDATTAEMVKLMEN
                     TYRDVNIAVANEFARLADRLGVDVWEAIGLANRHPRVNILRPGPGVGGHCISVDPWFL
                     VEAASDLTPLIRAAREVNDGQPQFVVDLVRRVAGQLNGCRIAALGLAYKPDVDDLRES
                     PAIEIVRLLGEAGARVLAYEPYKPDANISGITCVTSLQEALQDADLALLLVGHTALKD
                     VQPAQAATLMHGRLVVDVVNAWHSEAWAKAGFQLHRLGVGVL"
BASE COUNT          470 a          630 c          535 g          364 t
ORIGIN      
        1 agaaaggcta tcggtatgat taaggtgttg ggaaatctcg tttaccaacc ctaccgatgc
       61 ttccagtcct agctcgtagc cacccatcag gctgccctcc accaaaagaa tccgccagcg
      121 atcctgcgga ggctgaccag gtccaaccgg agagtaagcc gaaagatcca ccccattata
      181 aataacccgc caaggcacag cagtgggagc atagacctgt tcccaccagc ggcgggcgaa
      241 ttcgctctgg tagacaatgc gcccagccag acgggaacgg atgaaggata gcagccagtt
      301 gccatattcc gagcgcaggt aatggcgcaa acctgtccgc ctgagtcgat gcaaccagtt
      361 catcccgttg agacgttgca cgatgcgggc accacgtcgg cgggcgcgcc agagtcctgc
      421 taattggcgg gtgccaccga tcaccaggac agcgtcatag cgaccgtcgt caaggtcgtc
      481 acccacctcc acgccgcgct ggactaatcc agcagccagc cgccggcgga aggaaaccat
      541 cccgccaacg ccagagacct ttggggtgag gcagatacgg taatcaggca tacattatcg
      601 atcgcgtttc cccaccagct tggcaggcac gcctgcccac atctcccact caccggtgga
      661 ttgcaacaag acggcattgg ccccgatgac tgtgccgcga ccaacacgca gtaccccgtc
      721 tttgcacagc agcttgcatc cgggagagag gatgacatcg tcttcaatca cgatcccctc
      781 aaagcgggaa cgctccattg gcaggtgaat gtctgcccta cccaacccta ctcccgggta
      841 tatcttgacc cggtcgccaa tactgctgcg cggatggatc accaccccga agccgccatg
      901 cgccagctca aaatcttgcc caattttcac cgagcgaggc acctctacgc ccagaatttt
      961 tagggcgtaa tatgccaagc gccccaaaat tggccaggtg cgagcgtaga ctaggaagga
     1021 ataggcatcc atactgttcg atctacaata cacctacgcc aagccggtgt agctggaaac
     1081 cggcctttgc ccaagcctca ctatgccaag cgtttacaac atccacaacc aggcgcccat
     1141 gcattaatgt cgcggcctgt gcaggttgaa cgtccttgag ggctgtgtgc cccaccaaca
     1201 ataaagccag atcggcgtct tgcagtgctt cttgcagtga ggtaacacag gttatgccag
     1261 aaatatttgc gtcaggttta tacggttcgt aggcaagcac gcgcgcccca gcctccccca
     1321 gcaggcgcac aatctcgatg gcggggcttt cgcgcaagtc gtccacatct ggcttgtatg
     1381 ctagccccag agcagcgatg cggcagccgt tcaattgacc agcaacacgg cgcaccaaat
     1441 cgactacaaa ttgcggctgg ccatcgttga cctcacgggc agcgcgtatc aggggggtca
     1501 ggtcggaggc cgcttccacc aaaaaccacg ggtcgacgct gatgcaatga ccgcccacac
     1561 ctggtcccgg gcgcaaaata ttcacacgcg ggtggcggtt ggcaagtcca attgcttccc
     1621 acacgtcgac acccaaacga tccgccaggc gggcaaactc attggctacg gcgatgttga
     1681 cgtcacgata cgtgttctcc atcagtttga ccatctcagc cgttgtggca tccgtcaaga
     1741 tgatctcacc gcgcacgaag acctcgtaaa gatctctccc ggcttcggca gaagcacggt
     1801 tgaccccgcc aatgacgcgg gcgttctcga tcaactcgcg caggatctgt ccgggcaaca
     1861 cacgctccgg cgaataggcc aggtaaaagt caacgccggg atttagccca gagcgggcta
     1921 gaattggcgc taccacgtcc accgtggtac gcggagggga ggtcgattcg agaactacca
     1981 ggttgccagg gcgcaagtg
//
LOCUS       DBNHOH010000190         1686 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1549219, whole genome shotgun sequence.
ACCESSION   DBNHOH010000190 DBNHOH010000000
VERSION     DBNHOH010000190.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1549219"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="AC16V7_03400"
     CDS             <1..493
                     /locus_tag="AC16V7_03400"
                     /inference="COORDINATES: protein motif:HMM:NF013200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR6256197.1"
                     /translation="SWNNHALARAFLPLAQELQPDLVLTGVQAHNDLDGSLGPLVAAL
                     MGLPYVGYVAGVTVSDGVATIRKEYPAGLLAEMEVILPAVLGIQAAEQPPRYVAFSKV
                     RQSMKTTTLDERPAGESDPQGGVTVEHMFQPETGEGATMLEGDLTQVTDKLVSILHEL
                     DVI"
     gene            518..1498
                     /locus_tag="AC16V7_03405"
     CDS             518..1498
                     /locus_tag="AC16V7_03405"
                     /inference="COORDINATES: protein motif:HMM:NF013200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR6256198.1"
                     /translation="MNQDVFVSIEHLRGQVTDLSYGMLAAARQVAAEAGGEVVAVLLG
                     HTGQHLAQGLAADRVLSIDHPALADFTSEAYIQAWSYLLREYNPRLALFGDTSIGADV
                     AGGLSIRMGLPLVSACQVLLVENGELLFRSQICGGKIMAQGKLPPTTALVTMVPGGYR
                     AEEARGEEPSEIISLEAPPLEDLPVRLKEYIEPEISDVDITKENVLVAVGRGIQNQDG
                     IELVQELAEALGGVVCASRPVIDQGWLPITRLVGKSGGQVKPVVYLALGISGAPEHVE
                     GIVDSQVIIAVNTDLAAPIFNVAKYGLTCDLFDLVPILVDKIRLSRSIQV"
BASE COUNT          365 a          404 c          505 g          412 t
ORIGIN      
        1 tagctggaat aaccacgctc tagcgcgcgc ttttctacca ttagcgcaag agctacagcc
       61 tgacctggta ttaaccggtg tgcaagctca caacgatctg gatgggtcgc ttggacccct
      121 cgtagcagcc ttgatgggat tgccctatgt gggctatgtg gctggtgtca ctgtaagcga
      181 cggggtagcc accatccgta aggaatatcc tgccgggctc ctggcagaaa tggaagtgat
      241 cctgccggct gtattgggta ttcaggcggc agagcagcca ccgcgctatg tagcctttag
      301 caaggtgcgc caatcgatga agaccaccac actcgatgag cgcccggcag gagaatcaga
      361 cccgcaaggt ggagtgactg tggagcacat gttccagcct gaaactggcg aaggggcaac
      421 catgttggaa ggcgacctca cccaggttac agataagttg gtttccattc tccacgaatt
      481 ggacgtgatc tgattcgctc tgaagaggag tttagcgatg aaccaggatg tatttgtttc
      541 gattgaacac ctgcgtggtc aggtgactga tctctcgtat ggaatgctgg cagccgctcg
      601 ccaagttgcc gctgaagcgg gtggggaggt agttgctgtt ttgctaggcc atactggtca
      661 acatctggct cagggattgg ctgctgatcg tgtgctgtct attgaccatc cagctctggc
      721 tgattttacg tcggaggcgt acatacaagc ctggtcttac ttgcttcgag aatacaatcc
      781 gcgcctggcg ttgtttggcg acacgtcaat tggggcggat gtggctggtg gactttcaat
      841 tcggatgggg ttaccactgg tgagtgcttg ccaggtattg cttgtggaga atggagagtt
      901 gttgtttcgc agccagatct gtggcggcaa aatcatggca cagggtaaat tacccccaac
      961 caccgctctg gtaacaatgg tccccggtgg ttatagggca gaagaggcgc gtggcgaaga
     1021 accctcggag attatctctc ttgaggcccc gccccttgaa gacttgccgg tacgcctcaa
     1081 agagtacatc gaacccgaga ttagcgatgt ggatatcacc aaggagaatg tgctggtggc
     1141 agtggggcgc ggtatccaga accaagatgg catcgaattg gtgcaagagt tagctgaggc
     1201 cttaggtggt gtggtatgcg cttcacgccc ggtaattgat cagggttggc tgccgataac
     1261 caggttggtg ggcaaatccg gcgggcaagt caagccggtt gtgtaccttg cgctgggaat
     1321 cagcggagcg cctgagcatg tcgagggcat cgttgatagc caagtgatca tcgccgtcaa
     1381 tactgacctg gctgcaccga tcttcaacgt agctaaatat ggcctcacgt gcgatctatt
     1441 tgatttggtg ccaatccttg tagacaaaat tcgtctatct cgttcgatcc aagtatgagc
     1501 tgggagttca ggttaaaacc atgccgattc gtgagacctt ctggaacatt cctcaatggg
     1561 ccgagattgg tcaatatatt cttggcttgc tttcgctgct gatactgatc tatggtgtgg
     1621 tccagcgcat tcgacgctgg cggcaggggc agccggaaaa tcgctctgac cagctactgg
     1681 ttcgct
//
LOCUS       DBNHOH010000191         2798 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_395607, whole genome shotgun sequence.
ACCESSION   DBNHOH010000191 DBNHOH010000000
VERSION     DBNHOH010000191.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_395607"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="AC16V7_03410"
     CDS             <1..403
                     /locus_tag="AC16V7_03410"
                     /inference="COORDINATES: protein motif:HMM:NF019413.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HIR6256199.1"
                     /translation="YSAYRKEFDSSPGTYYLSKGWLESGSNPLSEYKEYEARLGSEKA
                     MWVMDMQYQNYKRLVMVAHTQEDLEKYRPMALDVARFCERWGMRYEEILGSDSFVRKL
                     IEVASLPDQANEEFVVIPPGGELKQSAFIRW"
     gene            490..1611
                     /locus_tag="AC16V7_03415"
     CDS             490..1611
                     /locus_tag="AC16V7_03415"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014686340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR6256200.1"
                     /translation="MKRTPFLLFLLWIMALPAACATGNTGSPVPSSTIQAVSTSLPPP
                     TATFGPSLTPTFTSTPTPTTTWTATYTPTPTLHPMHILAMRQLSYPGSEIVLEEQLDR
                     GANYSRYRASYLSEGFKIYALLTIPDGEIPPTGWPAIVFNHGYIPPAQYRTTERYIAY
                     VDWLARSGYIVFRIDYRGHDRSEGEANGAYGNTGYTIDVLNAVASLKQFSQVDPDRIG
                     MWGHSMGGYLTLRAMVISPDIKAGVIWAGVVGSYPDLLNRWRRRGGTIPTQAVTSSRR
                     WRVSWLEQFGSPEENPEFWNGISSNAYLDDISGPIQLHHGSADESVPLEFSSILFEQM
                     QQAGQPVELYIYEQDNHNLSKFFSQAMTRTIEFFNRYLK"
     gene            complement(1634..2644)
                     /locus_tag="AC16V7_03420"
     CDS             complement(1634..2644)
                     /locus_tag="AC16V7_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR6256201.1"
                     /translation="MSPSADHNHSISSATENYLESVALARSQNTARTYRNALSFFLQV
                     LAQHNHSPKYDPISELPEDAISWFAAALKGYAPATERLYLTAAISFFEYLAAERLADV
                     NLPRVRLLVRQRARRPGQRLPQFSRNTIEDVVKYVINIANLPTDTEPERLRNLRDRAF
                     LITLADSGLRVHEACGLRRGDIDWREGRAVIIGKGDQQAVVRFSRRALNTLRDYLAAR
                     AALDGASGRPLASLPIFARHDRGAGKKVKPITTATGRNIVNQRVREALGLSAEDEEMV
                     DAITPHSFRHFFVTTVLRGSGGNLKLAQELARHKNIAVTQRYAHLSDDELDQGYWEIF
                     EK"
BASE COUNT          683 a          676 c          735 g          704 t
ORIGIN      
        1 atacagcgca tatcgcaagg aattcgactc ttcacctggg acgtattacc tctccaaagg
       61 ctggttggaa tctgggagca atcctctgtc agaatacaaa gaatacgaag cgcggcttgg
      121 ttcagaaaaa gccatgtggg tgatggatat gcaatatcaa aattacaaga ggcttgtaat
      181 ggttgcccat acacaagagg atttagagaa ataccgaccc atggcactgg atgtggcacg
      241 attttgcgag cggtggggga tgcgttatga agaaatcctg ggatctgata gcttcgtccg
      301 caaactcata gaggtggcga gcttgccaga tcaagcgaat gaagaatttg tggtcatccc
      361 cccaggaggg gagctcaagc aaagcgcttt tatacgctgg taagctttgc agttggatga
      421 atatagggaa agctacttct ttctctatct aaatacggtt tgatgagtag ttttttattg
      481 gtgataccca tgaaaagaac cccgtttttg ctatttttat tatggataat ggccctgccc
      541 gctgcgtgtg caactgggaa taccgggagc ccggttcctt ctagtactat ccaagctgtt
      601 tctaccagtc taccgccccc tacagccaca tttggccctt cgcttacgcc tacgtttaca
      661 agcactccca cgccaacgac tacctggact gcaacgtata cacccacgcc gacgctacat
      721 cccatgcaca tcctggcgat gcgtcaattg tcctaccctg ggagtgagat cgtgcttgaa
      781 gagcagctgg atcgtggggc aaattacagc cgctacagag catcctatct atcggaaggt
      841 ttcaaaatct atgcattgct gaccatacca gatggtgaaa tacccccaac aggctggcct
      901 gcgattgtct tcaatcatgg ctatattccg ccagcgcaat accgaactac tgagcgctat
      961 atagcttatg tcgattggct tgcccgcagc ggatatatag tatttcgcat agattatcgt
     1021 ggtcatgacc gctccgaagg agaagcgaac ggtgcttatg ggaatacagg ctatacgatt
     1081 gatgtgctta atgctgttgc ttcactcaag cagttttctc aagttgatcc agatcggata
     1141 ggcatgtggg gacactcgat gggtgggtat ctcacgttga gagccatggt aatctcccca
     1201 gatatcaagg ctggggttat ctgggcgggc gtggtcggat cctatccaga cttacttaat
     1261 cgttggcgca ggaggggtgg aaccatccca acccaggcgg ttaccagttc tagaaggtgg
     1321 cgagtaagtt ggctggaaca gtttggctca cccgaagaga accctgaatt ttggaatggg
     1381 atttcaagca atgcgtatct ggatgatatc tccggcccca ttcagcttca tcacggcagt
     1441 gccgacgaaa gtgtgccgct cgagttttcc agcattcttt ttgaacaaat gcaacaagcg
     1501 gggcaaccag tggagcttta tatctacgag caggacaatc acaatttgtc gaaattcttc
     1561 agccaggcga tgacgcgcac aatcgaattc ttcaatcgct acttgaagtg attctaaaat
     1621 tgtccgcaac caattatttt tcgaaaattt cccaataccc ttgatctagc tcgtcatcgc
     1681 ttaagtgcgc atatctttgg gttacggcga tattcttatg ccgggcgagc tcttgtgcta
     1741 gcttcaggtt gccaccggag ccgcgcagca ctgtggtcac gaaaaagtgg cgaaaggagt
     1801 gcggcgttat tgcgtctacc atttcttcgt cctccgcgga caagcctagc gcctcgcgca
     1861 cccgctgatt aacgatgttg cgtcctgtgg ccgttgtgat cggcttgacc tttttgccgg
     1921 cgccgcgatc gtgacgggca aatatgggta gggaggctag cgggcgcccg gatgcgccat
     1981 caagcgcagc tcgggctgcc aggtaatcgc gcagcgtatt cagagcacgg cgtgagaagc
     2041 gtacgactgc ttgctggtct cctttaccga taatgaccgc tcgcccctcg cgccaatcga
     2101 tatcgccgcg gcgcagacca caggcttcgt gtactcgcag gccgctatct gccagagtga
     2161 tcaggaaagc gcgatcccgc aagttcctta gtctctcagg ctcggtatca gttggtaaat
     2221 ttgcaatatt aattacatat ttaactacat cctcaatggt attgcgggag aattgcggca
     2281 aacgctggcc tgggcggcgg gctcgctggc gcaccagcag gcgaacacgg ggtaaattga
     2341 cgtcagccag acgctcggct gccaggtatt cgaagaaact gatcgcagcg gtcaagtaca
     2401 ggcgctcagt ggcgggtgcg taacctttga gtgcggctgc aaaccaggaa atggcatcct
     2461 ctggtagctc agatattgga tcgtacttgg gggagtggtt gtgctgagca agaacctgta
     2521 gaaagaatga cagtgcgtta cgataggtac gtgcagtgtt ttggcttcgt gcaagcgcca
     2581 cgctctctag gtaattctca gttgcagagg aaatagaatg gttatgatca gcagaagggc
     2641 tcatgatgcg tgttcttcct gagataataa tgtttatgat aataatactt atcaaaagca
     2701 taaatattat agcaaggaat ttttgtttgt caacccccct gctctagtgt tccaaacaat
     2761 tctcgcggaa ttggtctcac caaagccaat tggcggta
//
LOCUS       DBNHOH010000192         2470 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_844694, whole genome shotgun sequence.
ACCESSION   DBNHOH010000192 DBNHOH010000000
VERSION     DBNHOH010000192.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_844694"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="AC16V7_03425"
     CDS             <1..222
                     /locus_tag="AC16V7_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256202.1"
                     /translation="LSLTLKARVNRLVNIIVGILHILVLLGTQFVGEGETWFYWRLYE
                     LLEALFLALIIWTAWKWPTTDSQLQESAS"
     gene            612..1325
                     /locus_tag="AC16V7_03430"
     CDS             612..1325
                     /locus_tag="AC16V7_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6256203.1"
                     /translation="MTNTNYTAVPFEHSGYALLLWDSSIQSYINCYLLLRPPHTILID
                     TGKVEHLTALIATLSNLGVAPEDVTHIIATHGHQDHIGGAAGFPFAHKHIHAHDAPLL
                     AELSLPEFIPNLPDDGALLGLQCHLLGQHTPGSIALFDPKSRAVFCGDHICFFGDALP
                     EDGPVSYGGKLRQITYEYFHKWMRGPERLDDNDLALFLKGLATLATFEAEVLCTGHGA
                     ILIRDITLFLALLQEAASR"
     gene            1584..2300
                     /locus_tag="AC16V7_03435"
     CDS             1584..2300
                     /locus_tag="AC16V7_03435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR6256204.1"
                     /translation="MTKLVGQKAVQRSSPRQWIGLIVVYLSIPLVLFVCGGDFGWWQA
                     WVYSLLIVAAGVGGRILAERRHPGLSAERQNMESIQEAKAWDKVLAPLMALSVGFPLV
                     IVAGLDHRFGWSPVFPLWLIVLGIFLIALGYAFSVWALVENRFFSSVVRIQTDRGHVV
                     CDSGPYRIVRHPGYAGNILPLLGIVLALGSLWTLIPAVVALVIAVIRTALEDRTLQEE
                     LPGYQEYARRVRYRLFPGIY"
BASE COUNT          531 a          614 c          671 g          654 t
ORIGIN      
        1 ctgtccctta cgttgaaggc cagagtgaat cgcttggtga acatcatcgt aggtattctc
       61 cacatccttg tcctgttagg tacacagttt gtgggcgagg gcgagacttg gttttattgg
      121 agactttatg agctgctgga agccctgttc ctcgcgctca tcatctggac cgcatggaag
      181 tggcctacta ctgattccca acttcaggaa tctgcttcct gatcgatgat cctgtgctcc
      241 tcatccgcat tagcaacacc tatcgtccca atctcactgc ggatcaactc tttgacacat
      301 cccgaggatc gcggcttgtg ggataaagca cggagaaggc cagatctgtt gctcatggaa
      361 agtgccccga cgtgagaggg cttgttccaa ggagaggtgc gtattaatgc atcattacag
      421 gtatgagcgg gttcgtgctc ttttcgccgt ctaaccattt gctggagccc tggactgtcc
      481 gtccaggaca ggcagacctg agattcggcc cgttttgcct agagagggca tattatcggg
      541 gttgagggca ggctgctcag ctcaaagccg tttggtggcc acctatgacg ataagagatg
      601 gtaagaattc gatgacaaat accaattaca ctgctgttcc gtttgagcac tccggctacg
      661 ctctcctgtt gtgggattct tcgatccagt catacatcaa ttgctatctt ctcttgcgcc
      721 ctccacacac aatacttatt gatactggta aggtagagca cctcacagcg ctgatcgcta
      781 ccttgagcaa tcttggcgta gcacccgagg atgtcactca tatcatcgca actcacggcc
      841 accaggatca tatcggagga gctgctggat ttcccttcgc tcacaagcat atccatgcac
      901 acgatgcacc gttgctagcg gaactcagtc tcccagaatt cataccaaat ttacccgacg
      961 acggcgcatt gctcggcctg cagtgccatt tgcttggaca gcacaccccc ggatcaattg
     1021 ctctctttga tccgaaatcc agagctgtgt tctgtggaga tcacatttgc ttcttcggcg
     1081 atgcacttcc tgaagatgga cctgtctctt atggtggcaa actaaggcag atcacctatg
     1141 aatactttca taaatggatg cgtgggccgg agcgcttaga cgacaacgat ctcgccctgt
     1201 ttctaaaggg tctcgcaact ctggccacat tcgaggctga agttctatgt acagggcacg
     1261 gcgccatcct aataagggat ataactttat tcttggcact gctccaagag gcagctagca
     1321 gataaggaat tcggtggcta cctaacaata tgtgcactgg atagcgagat tctgcagctt
     1381 tttcgggtat tttccacgcc caagcggtat cctgctctca agccaagtcc aagtccgccc
     1441 ccggtccgcc ggtaacgcaa gccattgggc gaattattga aggctggaac ttagtggtag
     1501 gcaagggcgg aattgtttac ggtgaattca aaaagcagta tgattgaagc gctggctgat
     1561 tcttgcgtgg tttggaggat ggtatgacga agttagttgg tcaaaaggcg gttcaaagaa
     1621 gttcccctcg ccagtggatc ggtttgatcg tggtgtatct ctccatacca ctggttctat
     1681 tcgtttgcgg aggagatttc ggttggtggc aggcatgggt ctattccctg ttaattgttg
     1741 ctgctggtgt gggagggcgt attttggcgg aacggcggca tccgggattg tcggcagaac
     1801 gacaaaatat ggagagtatc caagaagcga aagcatggga taaagtgctt gccccgctga
     1861 tggcgttgag tgtaggtttc ccactcgtca ttgtggcggg gctggaccat cgctttggtt
     1921 ggtcacctgt atttccgcta tggctcatcg tgctcggcat cttcttaatc gcgctcggat
     1981 acgctttttc tgtgtgggca ttggtggaga accgtttctt ttctagcgtg gtgcgcattc
     2041 aaacggatcg agggcatgtg gtgtgtgaca gcggtccata tcgaatcgtt cggcatccag
     2101 gctacgccgg aaatattctg ccactgctag gaattgtgct ggccttaggc tctttgtgga
     2161 cactcattcc ggcagttgtg gcgctggtca tagcggtgat cagaaccgca ttggaagatc
     2221 gaacgctaca ggaagagttg ccggggtatc aggaatatgc gcgacgcgtg cgttatcgat
     2281 tgtttccggg gatttactag gagacctgtg ttgatgtgag ataatgttct ggtcgctgcc
     2341 acaaaaaaag aatgcgccca acaccgcttg cacactgacg cggggcgggc ttcgcctcgc
     2401 ccgccaggga ttcagcgcct ttggccgggt actacctttg gcattttctc gccaaattcg
     2461 cctcgtccgc
//
LOCUS       DBNHOH010000193         4573 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1678256, whole genome shotgun sequence.
ACCESSION   DBNHOH010000193 DBNHOH010000000
VERSION     DBNHOH010000193.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4573)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1678256"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="AC16V7_03440"
     CDS             complement(<1..1027)
                     /locus_tag="AC16V7_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256205.1"
                     /translation="MSNPNRFPFAREIEIPSEMQGWEEMYPAHRMFSPERREWEETQF
                     WYADKIHAPDPMVPLDEVFQQAWQIALSQHTTRIFCIPPAQGIAQRMVGCYMYICAIT
                     PPPPEVIQEKAELFPKRTFPLFESYGTLWEDWLTRFQTLGKELRSIKVPTEFPKYMPD
                     FNSATTLPAVTPAQELLEAFNTAVNIIYRGWQYHFNYLNVTYLSYLMFADVARQLFPG
                     ISESTIGKMVAGAEVSMFRPEEELCRLARLAHAESKVADILRSDRPAASKIQSLRESE
                     AGRHWLDELERIKDPWMYVSCGAGWYHYEGSWITKLDVPFSYLKGYIERLDKGEKIER
                     SFVAIAEA"
     gene            complement(1235..2512)
                     /locus_tag="AC16V7_03445"
     CDS             complement(1235..2512)
                     /locus_tag="AC16V7_03445"
                     /inference="COORDINATES: protein motif:HMM:NF049415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HIR6256206.1"
                     /translation="MEIAVVGMLDEREPALRLLKERIERRGHKVTLIDVSIGTGGIMP
                     DLKPDITCEEVAQASGSSIEEIRKMLAKERDTATSLMAEGLAKKTLELYQAGRLNGML
                     GVAGMTGTFMTLTAMKSLPFGVPKVLISSVAAMPAYASRFAEYFSIRDITIIHSVVDT
                     VGWNPLVRSLMINGAGAICGMVEDVEPRLKEGKPSVAMTELGFCDKGAHYVRDLIQDS
                     YNIVSFHATGLGDRAAEDLVGQGLFDAFVDLVPAGVSERLLGGNRAAGPDRLEGACRQ
                     GTPYILAPCGFDMISCGPIERRDKGDPVWVSRKLADRKLLIQDAMRVQARTSVDEMAE
                     IAEAVAEKLNRHKDKRLVKFLIPTKGFSSLSVEGGQLYDPEVDRAFAQELRKRLAPEI
                     QVIEVDAHINEMAFAQAVAEALETSVRLRDSVK"
     gene            complement(2613..4013)
                     /gene="ppcB"
                     /locus_tag="AC16V7_03450"
     CDS             complement(2613..4013)
                     /gene="ppcB"
                     /locus_tag="AC16V7_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylphosphate carboxylase subunit beta"
                     /protein_id="HIR6256207.1"
                     /translation="MKDLRDYIETCEAEGELKRVKAEVDWNLELSHIAKLNEERSGPA
                     LLFENVKGYSSPVLTSACTTTARLALIMGMPKTSSLVEMMREWVKRSADKVPSKWVDS
                     APCKENIDKGDSVDLEKFPVPWVYPKDGGRYFGTAVYVVSKSPKTGWINLGTYRLQML
                     DKKTLGTQFIKGKHADIMLKEYGELKKPMPVAAIIGGDPLMFLMGAARLPAYESEYDF
                     AGAIRGTPIDVIKGETVDLPVPATAEIVVEGEVDPVAMLPEGPFGEYTGYYSGIGTTP
                     RPFIKVNCVTYRNNPILWSTTVGRAVTDTHMTMALTYGATLWQELVRMRIPGVKAVYC
                     PPEGSGRFIAIISVKQMYPGHAAQVGTAAISTEMGAYGLKTVIVVDDDIDPWDLPRVL
                     WAISFRFQPSRAEFIKRGRSTPLDPSLPLDAREITSRLIIDATIPYEWKEKPIPIDMD
                     PDMMKRVESRWSELGL"
BASE COUNT         1068 a         1234 c         1114 g         1157 t
ORIGIN      
        1 gtgcttcggc aatggcgaca aacgatcttt caatcttctc gcccttatcc aaccgctcaa
       61 tgtagccttt gaggtagcta aacggcacat ccaacttggt gatccaactg ccctcatagt
      121 gataccagcc agcaccgcac gagacgtaca tccagggatc tttgattctt tcgagttcat
      181 ccagccaatg ccggcctgct tcgctttccc taaggctttg aatcttggac gctgcggggc
      241 ggtcagaccg aagaatgtca gccactttcg attcggcatg agccagacgc gctaggcgac
      301 acagttcctc ttccggacgg aacatggaaa cttccgctcc ggctaccatc ttaccgatgg
      361 tgctttcgct gatgcctggg aagagttgac gcgccacatc tgcaaacatc aggtacgaca
      421 ggtaggtcac gttcaagtag ttgaaatgat actgccagcc gcggtagata atgttgacgg
      481 cagtgttaaa tgcctccagc aattcttgcg ccggcgtgac ggcgggtaga gtggtcgccg
      541 agttaaaatc gggcatgtat ttcgggaatt ctgtggggac tttgatggag cgcagttcct
      601 tacccagagt ctgaaagcga gtaagccaat cttcccacag ggtgccgtag ctttcaaata
      661 gcggaaaagt tcgtttggga aacagctcgg ccttttcctg gatcacctct ggaggcggcg
      721 gcgtgatggc gcagatgtac atatagcagc cgaccatccg ttgggcgatg ccttgggcag
      781 gcgggatgca gaatatgcgg gtggtatgtt gagaaagggc gatttgccag gcttgttgaa
      841 aaacctcatc gaggggaacc atcgggtctg gcgcgtggat cttgtcggca taccagaatt
      901 gcgtttcttc ccattctcgt ctttctggac tgaacatgcg gtgagcagga tacatctcct
      961 cccagccttg catctcagag gggatttcaa tttcccgcgc gaaagggaat ctgtttgggt
     1021 tagacatgct ttacctcctt ggctttgttg attgttttga ttgcctaatg tttgttattc
     1081 acatggtcag gcaattgagc aaaggttgat tgctgataca actatcaccg ctccagcagg
     1141 aaaccgcgta gaaaggcgca acacgcgcgg gttccccgta taacctgctc tcaatttata
     1201 ggatatccgt tatcctcacc tcctcgttat cacctcattt gaccgaatcc cgtaaccgca
     1261 cgctcgtctc cagggcctcg gcaacagctt gggcgaatgc catttcgttg atatgtgcat
     1321 ccacctcgat cacttggatc tcgggagcca agcgctttcg cagctcctga gcgaatgccc
     1381 ggtcaacctc cggatcgtat aattgtccgc cttcgacgct caaagaggaa aatcctttgg
     1441 tgggtatcaa aaacttgacc aagcgtttgt ctttgtgccg gttcaacttc tctgcaaccg
     1501 cctcagctat ttcagccatt tcatccaccg aggtgcgcgc ctggacccgc atcgcatcct
     1561 ggatcaaaag tttcctgtcc gccagtttgc gcgaaaccca aacgggatca cctttatccc
     1621 gccgctcgat gggcccacaa ctaatcatgt cgaatccgca gggggccagt atatatggtg
     1681 tcccctgacg acaagcgccc tccagccggt cagggccggc tgcgcggttg cctcccaaaa
     1741 gtctttcgct cacgccggct ggcaccaagt caacaaaagc gtcaaacaac ccctgtccga
     1801 ctaaatcttc ggcggcgcga tctcccaatc ctgtggcatg aaacgagacg atgttatagc
     1861 tatcttgtat caggtccctt acgtaatgcg cccctttatc gcaaaagcca agctcggtca
     1921 tggcgaccga gggcttgccc tctttcaatc gcggctccac atcttctacc atgccgcaga
     1981 tcgccccggc gccattgatc atcaaacttc ttaccaatgg gttccagcca acggtgtcca
     2041 caacactatg aatgatcgta atgtcgcgaa tgctgaagta ttcggcaaac cggctggcat
     2101 aagcgggcat ggcagctacg cttgaaatga gaaccttggg aacgccaaac ggcaaagatt
     2161 tcatggccgt caaggtcatg aatgtgccag tcatgcctgc tacaccgagc ataccgttca
     2221 gccgtccggc ttggtacaac tccaaggttt tctttgccag accctccgcc ataagggatg
     2281 tggcggtgtc tctttcttta gccagcattt ttctgatttc ttcgatggaa cttccactgg
     2341 cttgtgccac ctcttcacac gttatatccg gcttgagatc gggcataatc ccgccagtgc
     2401 caatgcttac atcaatgagc gtaaccttgt gtccgcgccg ctcaatacgt tccttgagca
     2461 ggcgcaaggc tggttccctt tcatccaaca tgcccacaac agcaatttcc acgttgatcc
     2521 tcgtattatt aatagttcct tcagtattcc gggttcgttc gttgatattt ctattaaacg
     2581 aaggagccgc ggaaactgaa ggaacctggg atttagagac ctaactcact ccatctggac
     2641 tcgacgcgct tcatcatgtc gggatccata tcgatgggga tgggtttttc cttccactca
     2701 tatgggattg tggcgtctat gatgagtcgc gatgtaattt cccgggcatc aagcggcaag
     2761 gatggatcga ggggggtgga acggcctcgc ttgatgaact cggcacgcga gggctggaag
     2821 cggaagctta tcgcccacaa aacgcgcggc agatcccacg ggtcaatgtc atcatccacg
     2881 acaatgacgg ttttgagtcc ataggcaccc atttcggtcg agatggcggc ggtgcccact
     2941 tgggcagcat ggccgggata catctgtttc accgatatga tggcgatgaa ccggcctgag
     3001 ccctccggtg ggcaataaac tgcttttacg ccaggaatgc gcattctcac caattcctgc
     3061 cacagggtag caccgtaggt cagggccatg gtcatgtggg tgtcggtaac tgcccggcct
     3121 actgtcgtag accataaaat cgggttattg cggtaggtga cacagttgac cttgataaat
     3181 ggtctgggcg tggtaccaat accggaatag tacccagtat attcgccaaa cggtccctca
     3241 gggagcatag ccacaggatc cacttcgcct tccaccacga tctcggctgt agctggaaca
     3301 ggcagatcaa ccgtttcgcc tttgatcaca tcgatcggcg ttccgcgaat ggcgccggca
     3361 aaatcgtact cagattcata agcaggcaaa cgggctgccc ccatcaagaa catcaatgga
     3421 tcgccgccaa tgattgccgc aacaggcatg ggctttttga gttccccata ttccttcaac
     3481 attatgtctg catgcttgcc tttgataaac tgggtaccca gcgttttctt gtccaacatc
     3541 tggaggcggt acgtgccgag attaatccag ccggtcttgg gagatttgga cacgacatac
     3601 actgccgtac caaagtatcg cccaccatct ttaggataga cccacggaac cggaaacttt
     3661 tccaggtcaa cactgtcgcc tttgtcgatg ttctccttac aaggggcaga atcaacccat
     3721 ttggaaggaa ccttgtccgc tgatcgctta acccactccc gcatcatctc aaccaaactg
     3781 gaagtcttgg gcatgcccat aatcaaggcc agcctggctg tggtcgtgca ggcgctggtc
     3841 aacaccgggc tggaatagcc ctttacgttc tcaaacaaaa gagccggccc actgcgctct
     3901 tcgttgagtt ttgcgatatg gctcaactcc agattccaat ccacctctgc tttgacccgc
     3961 ttgagttcgc cttccgcttc gcacgtctcg atatagtctc gcaaatcctt catttttttc
     4021 tccttttcta ccgttagtat ttgagttgca taacgttttg tgctattaac gggaagaatc
     4081 accagccagt gatctccgtt gctgctatgg cgatgttaag tctcggcgag cttatctgag
     4141 agtctccgca agcgagactt gcggaaacgt gtttgattgg tcaaatgcta ttcgatcaaa
     4201 tgggctgctt gctgcttaga cgacacttgt aaaggaactg ccacgcggag tgaattctcc
     4261 taaattatta accgataacg taagcaatac attgatttga tatttaataa agagaacaga
     4321 ctatagctgg gacagcaagc cagtcccagc ctatacaaag tgtttataac aatatgatgc
     4381 gtctgatcca agtgataaat atcctataaa aattgataaa agtaacatct tgcacaaaat
     4441 agattgtgaa atatttctca aactaactta gatgaagtca agttatcccg tccagtgtgc
     4501 acattcctga ccaacgccct ggtcataata ttgcattttc agtccttgtt ttagatgaag
     4561 tgcgatctaa atc
//
LOCUS       DBNHOH010000194         2714 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1678262, whole genome shotgun sequence.
ACCESSION   DBNHOH010000194 DBNHOH010000000
VERSION     DBNHOH010000194.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1678262"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="AC16V7_03455"
     CDS             <1..1031
                     /locus_tag="AC16V7_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256208.1"
                     /translation="SPSIVTPPEQVASAELGTQAMPAVEPSVTPSPGADETLPPVERG
                     AGGPPAPPPTATSAPSAGKKPLPMAVWVGAGLVVVILVIFGIIQASRGGSQPSQALEP
                     AASATPTTAVVVAAAPTDTLAPTETAAPSATATLAPTDTPSPTPTPTLGLPPTPTFPA
                     GVPFAFIKSITLDEGLNYIVDYDTLEFQEKLPGVHVHFFFNTVPPEQAGNPVSGPCIL
                     WGGPRPFDKYRQRDHNEQATQMCVLVANADHSVQPESGNCAILPDVNAVTILTDTACL
                     AGTDVSSQVVTSLPLGQIARVLGISTDGAWWNIANPEDMAANCWVPGDLAFFHGDQAS
                     VPVVEPVD"
     gene            1076..>2714
                     /locus_tag="AC16V7_03460"
     CDS             1076..>2714
                     /locus_tag="AC16V7_03460"
                     /inference="COORDINATES: protein motif:HMM:NF014503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR6256209.1"
                     /translation="MDTTYGKLILLNPNGPEQEFELAKASVSLGRAITNDIILSDVRI
                     SRNHARLEGGSQGCTLVDLDSSNGTRVNGIQIERATLKPGDTISLGNQLLRYEIADIS
                     EDLGLTQIDTQAQLEQTMDREFLPQAINETGNPSLVIFTGDRTWRVDLEDQATIGRDE
                     SCTVFIDAANVSRRHAEIVYTGGIYVLKDLGSTNGTWVRDQQVDQLILQDGDVMRIGP
                     AQIIFKGGFQEQALTMADEQLAKPTGRRTVVFVPGSMGSELWLGNERVWPNVKTIFTN
                     PEIFIYPSRVPLEPRGIVDEVVIVPNLIKQDQYNRLGDYLVEELNFRRGVDFFEFAYD
                     WRQDVRISARQLGELIQSLPDTQPLVLIAHSLGTMVSRYYIERLGGHKRIERAILMGG
                     PHQGSTKILTSMLIAPEVLPFGIMGERLRQIVLTFPSTYQIIPTYEVGTDQDGKKINF
                     LDDESWLAPEFVPLLRQGREFRSELGSMLSFPYVSVFGYGLKTISDVTIARDAAGKVQ
                     KVDYISGNAGDNSVLEQSAFLQGSEIHPVRQHHGSLFV"
BASE COUNT          570 a          794 c          796 g          554 t
ORIGIN      
        1 acagcccatc cattgtaacc ccgccagagc aggtggcaag cgcagagctt ggcacgcagg
       61 ctatgccggc cgttgagccg tcagtgacgc cctcccccgg tgcagatgag accctcccgc
      121 ctgttgagcg tggtgccggc ggaccgcctg cccctccgcc gactgcgaca agtgctccga
      181 gcgcgggcaa gaagccgctg ccgatggcgg tatgggttgg cgcaggcctg gtagtcgtga
      241 tactggtcat ctttgggata atccaagcca gccgtggggg atcgcagccc agtcaagcgc
      301 ttgaaccagc agccagcgcc acgccaacta cggcggttgt ggtggccgcg gctcccaccg
      361 acaccctcgc cccaaccgag acagccgctc ccagcgccac cgctaccttg gctcccaccg
      421 atacaccctc gcctaccccc acgcccacgc tagggttgcc ccccaccccc acctttcccg
      481 ctggcgttcc ctttgctttc atcaagagca ttacactgga tgaagggcta aactatattg
      541 tcgattatga cacgctggag ttccaggaga agctgcctgg ggtgcacgtg catttcttct
      601 ttaacaccgt cccgcctgag caggctggaa atccagtatc aggaccctgt atcctgtggg
      661 gcgggccgcg gccctttgat aagtacaggc agagggacca caatgagcaa gccacccaga
      721 tgtgcgtact ggttgccaat gccgatcaca gcgtccagcc ggaaagcgga aactgtgcca
      781 tcctgccgga cgtcaacgcg gtcacgattc tgaccgatac tgcttgcctg gctggaacgg
      841 atgtgagctc ccaggtggtc accagcctgc cgctcgggca gatcgcgcgt gtcttgggaa
      901 tttcgaccga cggagcctgg tggaatatcg ccaatcctga ggatatggcg gcaaattgct
      961 gggtgccggg cgacctggcc ttttttcacg gcgaccaggc gtcggtgcct gtcgttgaac
     1021 cagttgatta attcgttaat tgcgtaactt ctgaagattc tgctcaggag ggaagatgga
     1081 caccacgtat gggaaattaa tcctgctgaa ccccaacggt ccagagcagg aatttgaact
     1141 ggcaaaagcc agcgtctcgt tggggcgggc gatcactaac gatattatcc tcagtgatgt
     1201 gcgtatttcg cgcaatcacg ctcgcctgga aggtggctcg caaggttgta ccctggttga
     1261 tctagactct tccaacggca cgcgggtcaa cggtatccag atcgagcgcg ccacgctcaa
     1321 acctggcgac acgatcagcc tgggaaacca gctgcttcgc tatgagatcg ctgacatcag
     1381 tgaagacctg ggcctgaccc agatcgacac acaggctcag cttgaacaaa cgatggatcg
     1441 ggaatttctt ccccaggcga tcaacgagac cggtaacccc agcctggtga tattcaccgg
     1501 cgataggacc tggagagtcg acctggagga ccaggctacg atcgggcgcg acgagagctg
     1561 cacggtcttc attgacgcgg ccaatgtctc ccggcggcat gctgagatcg tctatacggg
     1621 gggcatctat gtgctcaaag acctggggag cacgaacggc acatgggtgc gagaccagca
     1681 ggttgaccag cttatcctgc aggatggtga tgtaatgcgg attggcccag cccagatcat
     1741 tttcaagggc ggatttcaag agcaggcttt gaccatggct gatgagcagc tcgccaagcc
     1801 caccggccgg cggacagtgg tgtttgtccc aggttcgatg ggctcagagc tctggttggg
     1861 aaatgagcgc gtctggccga atgtcaagac gatcttcacg aacccggaga ttttcatcta
     1921 cccatccagg gtcccgttgg agccgcgcgg tatcgtggat gaagttgtaa ttgttccaaa
     1981 cttgatcaag caagatcagt acaatcgcct gggcgactac ctggtggagg aattgaattt
     2041 tcggcgcggg gtggatttct tcgagttcgc ttacgactgg cgccaggatg tgcgcatctc
     2101 ggcccgccag cttggcgagt tgattcagag cctgcccgat acgcaacccc tggtcctcat
     2161 cgctcacagc cttggcacca tggtcagtcg ctattacatc gaacgcctgg gcgggcacaa
     2221 gcgtatcgag cgcgccatcc tgatgggggg accgcatcag gggtctacca aaattctcac
     2281 cagcatgctg atcgcgcccg aagtgctgcc gtttggcatc atgggagagc gattgcgcca
     2341 gattgtgctg accttcccca gcacgtacca gatcataccc acctatgagg tgggcaccga
     2401 tcaggacggt aagaagatca acttcttgga cgatgagagc tggctggcgc cagagtttgt
     2461 tccgttgttg cgacagggca gagaattccg cagcgagctg ggcagcatgc tgagcttccc
     2521 atatgtctcg gtcttcggct atggcttgaa gaccatttct gatgtcacaa tcgccagaga
     2581 cgctgccggt aaagtgcaaa aggtggatta catcagcggc aatgcgggtg ataactctgt
     2641 cctcgagcag agcgccttct tgcaaggcag cgaaatccac cctgtgcgcc agcatcacgg
     2701 ctccttattc gtcg
//
LOCUS       DBNHOH010000195        10869 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_652243, whole genome shotgun sequence.
ACCESSION   DBNHOH010000195 DBNHOH010000000
VERSION     DBNHOH010000195.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10869)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10869)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10869
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_652243"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1390)
                     /locus_tag="AC16V7_03465"
     CDS             complement(161..1390)
                     /locus_tag="AC16V7_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003596089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HIR6256210.1"
                     /translation="MTNPSVALKEYLINIGLDEDVDFLRESVQLLSQMLIELEAEEQI
                     GADKHERTPQRNNHRNGYRQRLWETRVGEIDLRIPKLRKGSYYPSLLQPRRRTEKALL
                     AVVQQAYVEGVSTRKVDDLLKALGLTGIDKSKVSRICKELDEVVEQFRNRPLEGEYPY
                     LWLDALYLKVRQNHRIVSLAMVLAIGVDTQGRRHILGFGLGASEESAFWLDFLRSLVE
                     RGLQGVQLVISDAHEGLKAAISQVLTGATWQRCRVHFMRNVLAHVPKRDKALVAAAIR
                     TIFAQPDREAARQQLVEVVKAMRKRWSKAAEILERAEDDILAYMAFPQKHWTRIYSTN
                     PLERLNKEVKRRTNVVGVFPDQPAVLRLAGSILMEIDDEWQAADRRYFSLETMRELTH
                     PEVLLEDLGPLRLAPIH"
     gene            1831..2793
                     /locus_tag="AC16V7_03470"
     CDS             1831..2793
                     /locus_tag="AC16V7_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256211.1"
                     /translation="MKNLSFVFILLVLAACAPVDALQLPAGEVGSLEGQSQMPVETSP
                     RIQMGDGRTVEIIGGDEDTLREFVQRWLIPVYPGAPGGVTTVRFGALPEILPVEIPIP
                     KGAHIVASVQEPNSFTQIILDVDLSSDEITAFYSQSLDGSGWQSAPQESHSGFVGSGD
                     LPARYCLESDKAYLEVWAIETSDGPTDVRLNLYASMVSNFCQASGPGLMDEGMSLIPS
                     LKAPAGTQTLGGGAGSSGDGSAYVSTDLDSSLTVEALLEHYNAQLVAGGWELVEQGTT
                     PVVAWSTWELADMDGDVWGGTLFVMKKHMTADRLFAMLSVDKTP"
     gene            2828..4708
                     /locus_tag="AC16V7_03475"
     CDS             2828..4708
                     /locus_tag="AC16V7_03475"
                     /inference="COORDINATES: protein motif:HMM:NF016202.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HIR6256212.1"
                     /translation="MATHTTTRRMSVPTPGPVPQVFIALVGGLILFFMLSLGAVITFD
                     VIHAGRVYPGVSVAGVDLSGLRPHEATTLLAQRLTYPVTGNIVLKLGDKVWVAKPAEM
                     GLFFDAQTSANVAFSTGRQGNPLRRLSEQFNSWYRGVDVSPLLVYDQRAAYRYLEAIA
                     AQVDIPTVEASLSVNGTDVDVRPGQVGRSVDIEATLAPLEGQLRSMSDGLIPVVMVET
                     PPIILDASAQAEIARKILSAPLNLIVPDAAEGDPGSWTFDQQSIAQMLSIERVETPEG
                     MRYQVGLSAENLRGFLQGVSAELDRQPANARFIFNDDSRQLEVIQSAVIGRSLNVDAS
                     IQEINQKAVQGEHNIPLVFDYTQPAVGNDATAEQLGITELTSVQNSYFYGSSSSRIQN
                     IQTAAARFHGLLVPPGATFSMADALGDVSLDNGYAEALIIFGNRTIKGVGGGVCQVST
                     TLFRTAFFGGYPVVERYPHAYRVSYYELTRSGSVDTDLAGLDATVFVPVVDFKFTNDT
                     PNWLLMETYVKPEARVLTWKFYSTSDGRTVDWDSTGLENVEEPPEPLYQENEELARGE
                     IRQVDWEAAGADVVVYRTVHRDGQVYLQDEFVTHYMPWRAVYEYGPGTKIPKDKRNND
                     ED"
     gene            4797..5012
                     /locus_tag="AC16V7_03480"
     CDS             4797..5012
                     /locus_tag="AC16V7_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HIR6256213.1"
                     /translation="MVSQELLEILRCPNCVREKPGLLEYYKEAWLICQDCGRKYPIRE
                     DIPVMLIDEGDNWINVAKEDLPVPPPG"
     gene            5077..5676
                     /locus_tag="AC16V7_03485"
     CDS             5077..5676
                     /locus_tag="AC16V7_03485"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR6256214.1"
                     /translation="MMTLDELLLELTSGDDERAEGAVRSLSGYGEAALDALRSMLASP
                     DVDRRWWAVRALAEIPGGAAIPFIIQALSDEDPAVRQCAALGLCQQPDIPDDSVAEVV
                     VPALIEAMASNDALLARLAANALTCIGAAAVPALLDLLANSPDPVRLEAARALALIGD
                     TRAIPALFEALEDDSALMEYWANEGLERMGVGMVFFQPE"
     gene            complement(5703..6215)
                     /locus_tag="AC16V7_03490"
     CDS             complement(5703..6215)
                     /locus_tag="AC16V7_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019009679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HIR6256215.1"
                     /translation="MKTLYGWYLRVDQRITHWMARYGVLLLRISLGIVFFWFGVLKFF
                     PGLSPAQDLAARTIEQLTFGLITAKVSLPILAAWECIIGLGLISGIWMRATLFLLFMQ
                     MMGTITPVFLFPQEIFLHLPYAPTLEGQYIIKNLVIISAGIVIGATVRGGGVVDDPAL
                     VRLSHKPAHR"
     gene            complement(6223..6720)
                     /locus_tag="AC16V7_03495"
     CDS             complement(6223..6720)
                     /locus_tag="AC16V7_03495"
                     /inference="COORDINATES: protein motif:HMM:NF016213.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HIR6256216.1"
                     /translation="MPDWINASIFALTQVIMLVGLFGLIVPIFPGTVIIWLAALGYGV
                     VSGFGTLGIVLFVIITILMLAGTLADNVFLGAGARKGGASWKTIAIALVAGVLGTLIF
                     PPFGGLIAAPLAVLLLEYQRTGNLEKAWGALRGMAAGWGMSFIVRFAIGFVMMSLWWI
                     WAWKG"
     gene            complement(6730..8004)
                     /gene="serS"
                     /locus_tag="AC16V7_03500"
     CDS             complement(6730..8004)
                     /gene="serS"
                     /locus_tag="AC16V7_03500"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIR6256217.1"
                     /translation="MIDINLIREATDRVRQAMAVRQMESTLVDQALELDEKRRLLIQE
                     VETLKAERNAASKEIGRMKDPTERQAKIEAMRLVGDRITALDESLREVEGQLDGVMAL
                     IPNIPDPSTPLGKDENENVLIKTVGELPKYSFEPKPHWELGPALGIIDFEQGVKITGS
                     RFYVLSGAGARLQRALIAWMLDLHSRQGYTEKYPPFMVKGQTLFGAGQLPKFQDNLYR
                     DHEEDLWMVPTAEVPLTGLHMDEILDESALPRYYTAYTPCFRREKMSAGRDVRGIKRG
                     HQFDKVEMYIYCKPEDSPAAFEKMVADAEQTCAQLGLPYRVKQLCTGDLGFGSTITYD
                     IEVWAPGCGEWLEVSSVSNVTEFQARRANIRYRPADVGRPRFVHTLNGSGLGMPRTLI
                     GVLENYQQDDGSVVVPEVLRPWMGGVDVIRAA"
     gene            8428..8799
                     /locus_tag="AC16V7_03505"
     CDS             8428..8799
                     /locus_tag="AC16V7_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256218.1"
                     /translation="MLRRIELIHPAEKTTLPDDSIGFFPAIKLGLMGSINPKIVEAWS
                     DKLRGPGKRQSGIRRNARFYFTELGWKEVGRKVVKACQQVGQEYRVIKVKEGTVNVVW
                     RDRHYGLEVAAQPRKRRHNRR"
     gene            8804..9322
                     /locus_tag="AC16V7_03510"
     CDS             8804..9322
                     /locus_tag="AC16V7_03510"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6256219.1"
                     /translation="MDEKIEIRSLDEGDAEVFRALHLRALDELPEAFLAVYEEELCEP
                     LERVAERLRGSKPENFYLGVFVNGHLVGMVHFERPGSIKIRHRAYLSGMYVAPERRGQ
                     GLGKALLDRALAHARSLPDLEEVGLWVIIGNQQAEALYQSAGFETFCIEPRSIKIDDR
                     YYDGAGMIMRLR"
     gene            9319..9750
                     /locus_tag="AC16V7_03515"
     CDS             9319..9750
                     /locus_tag="AC16V7_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256220.1"
                     /translation="MSMDFIKENADSLARLKAFTDRVSLSQLEQRLESGWTVAATLAH
                     MAFWDQRASLLVRRWRENGVDSSPVDVDVINDASKALCHALLPKQTIQLVLESAEAID
                     REIEGISPALAEEIEAGATQFHLSRANHRDYHLDEIEAALE"
     gene            complement(9810..10322)
                     /locus_tag="AC16V7_03520"
     CDS             complement(9810..10322)
                     /locus_tag="AC16V7_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012964099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR6256221.1"
                     /translation="MDYFELLEKRHSIRSYTSQPVEDEKLHAILQAANRAPSAGNLQA
                     YEIYVVTDPARLQALAWAALDQTFIAQAPVALVFCASPKRSSVRYGRRGTTLYCIQDA
                     TIACAFAHLAAAALGLGSVWVGAFDEDGVRRVIGVWEDLRPVAILPVGYSSEKPEITS
                     RRQLSSLVHR"
     gene            complement(10348..>10869)
                     /locus_tag="AC16V7_03525"
     CDS             complement(10348..>10869)
                     /locus_tag="AC16V7_03525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HIR6256222.1"
                     /translation="RRSLRQFSDDPLTLEQIGQLLWAAQGITHPAGLRSAPSAGALYP
                     LEVYAVTQQAVYHDLPQDHCLSVQIQGDARAALYEAALKQGAVREAPAVIVIAAVYAR
                     TVKKYGEERSPRYVHLEAGHAAQNILLQAVALDLGAVPIGAFYDDQVQQVLELPPDHQ
                     PLYLIPTGHALIQ"
BASE COUNT         2307 a         2987 c         3171 g         2404 t
ORIGIN      
        1 gtattaattg ttcgatattt gtggacatcg ttatcacccc tactttatta ttctaaatgg
       61 cgctagcatg aaatgatttg aataactctt gttagtgtct cagtcaatgg tgtaaaagta
      121 cttgtgcact ccgatgagtc aaccgaatat tttttctttt ttagtgaatt ggcgccaatc
      181 tcaaaggccc caaatcttcc aggagcactt ctggatgggt cagttcacgc atagtctcca
      241 aactgaagta acggcgatcc gcggcctgcc attcgtcatc gatctccatc aggatcgaac
      301 ctgccaggcg caggacagca ggctgatccg gaaacacacc gaccacattc gtgcgacgct
      361 tgacctcctt gttgaggcgt tccagcgggt tggtcgaata aatcctggtc caatgcttct
      421 gaggaaacgc catatatgcc agaatatcat cttccgctcg ctccaggatc tcggcagctt
      481 tcgaccagcg cttgcgcatg gccttgacta cttccaccag ctgctgcctg gctgcttccc
      541 ggtctggctg ggcaaagatg gtgcggatgg cagccgctac cagggctttg tcccgcttgg
      601 gcacatgggc cagcacattg cgcatgaagt gcacccggca acgctgccag gtggctccgg
      661 tcaagacctg gctgatggcg gctttcaagc cttcgtgggc atcgctgatg accaattgca
      721 cgccctgcaa gccacgttct accaaagagc gcaagaaatc caaccagaag gcgctctctt
      781 cgctggctcc cagcccgaat cccaagatgt gacgccggcc ttgagtatct acgccgatgg
      841 ccagcaccat tgccaggctg acgatacgat ggttttggcg cactttcagg tacagcgcat
      901 ccagccacaa gtagggatat tcgccttcca gcgggcggtt gcgaaattgc tccaccacct
      961 catccaactc cttacagatg cggctcactt tactcttatc gatgccagtc aagcccagcg
     1021 ctttcagcaa atcgtccact ttgcgggtgc tcacgccctc cacgtaggct tgctgcacca
     1081 ccgccaggag cgctttctct gttcgcctcc ggggttgcag caaactcgga tagtagctgc
     1141 ccttgcgcaa cttcgggatg cgcaagtcaa tttcacctac ccgcgtctcc cacaaccgtt
     1201 ggcgatagcc gttgcggtga ttgttgcgtt gaggcgtgcg ctcgtgtttg tccgccccaa
     1261 tctgctcctc ggcttccagc tcgatcagca tttgactcaa caactgaaca ctttctcgca
     1321 aaaagtcaac gtcttcgtcc agtcctatat tgataaggta ctctttcaat gctacacttg
     1381 gattggtcat ctttgtacct cctttctgag tgtttggtca cacccatgga agcacaagat
     1441 gaccgttttt gtcaatcctt tttacaccac tctatgagac attatccttc gctcctcagt
     1501 atattgggaa gatgttcaga aattatgaag atgcgatttg gatattattt ggattcgtta
     1561 catacccata ctcatcctcg gatccccagt ctatgttttc cgggaaccat ttcacacaga
     1621 acaacgttaa tgaaacagtg ttacgcagtt ggggaggtga ttgggatacg ggcggcctta
     1681 cccgcctaaa ccttttgctt ccctaacatc gcgaacctca tgttcaaaca tgattattta
     1741 taatcgggcg aataaacaac ggcagaacac aggttaacac agatatcata acttttcggt
     1801 aaacgccttg aggctatgag gaggcatgtc gtgaaaaact taagctttgt attcatccta
     1861 ttggttttgg ctgcgtgtgc gccggtcgat gctcttcaat tacccgctgg ggaggtgggc
     1921 tctcttgagg gccaaagcca gatgccagta gagactagcc cgcgtattca aatgggtgat
     1981 ggtagaacag tggagatcat cggcggagat gaggatacgc tccgcgagtt cgtccaacgc
     2041 tggcttatcc cggtttaccc aggcgcgcct gggggtgtga ccacggtcag gtttggggca
     2101 ttgccggaga ttttgccggt ggagatcccc atccctaagg gggcacacat cgtcgcctca
     2161 gtgcaggagc ctaatagttt cacccaaatt atcctggacg tggacctgtc ctcggatgag
     2221 atcactgcgt tctactcgca aagcttggat ggatctggat ggcaatctgc cccccaagaa
     2281 tcacatagtg gtttcgtcgg ttcaggggat ttaccggcgc gctactgcct agaatccgat
     2341 aaggcctatt tggaggtttg ggctatagaa acatccgatg ggccgacgga cgtgcgcctg
     2401 aacctatacg cctcgatggt tagtaatttc tgccaggcga gtggtccagg cttgatggac
     2461 gaggggatga gtctgatccc atccctgaaa gcgcctgctg gaacgcagac gctcggcggg
     2521 ggtgcaggca gcagtggcga cgggagcgca tacgtctcta ctgatctcga ttccagcctt
     2581 acggtcgaag cgctattgga gcactataac gctcagttag tcgcaggtgg gtgggagctg
     2641 gttgagcaag ggaccacgcc ggtggttgcc tggagcactt gggaactggc ggacatggat
     2701 ggtgacgtgt ggggaggcac gctgtttgtc atgaagaagc acatgacagc agatcgcctc
     2761 tttgccatgt taagtgtcga caagacgcct taggcgatgc gatttttatt tggtacaata
     2821 ccccccaatg gctacgcata cgacaacccg gcgcatgagc gtgcccaccc ccggacctgt
     2881 accgcaggtt ttcatcgccc tggtcggcgg attgatcctg ttttttatgt tgtccctggg
     2941 cgcagttatc acctttgatg taatacatgc cgggcgagtg tatcctgggg tatccgtggc
     3001 gggagtagac ctttcgggct tgcgcccgca tgaagctacc actttgcttg ctcagcgctt
     3061 gacttacccg gtgaccggaa acatcgtcct caagctgggt gacaaagtct gggtggctaa
     3121 gcctgcggaa atgggccttt tcttcgacgc gcagacatct gcaaatgtgg ctttctctac
     3181 tggccggcaa ggcaacccat tgcgcaggct gagcgaacag ttcaattcat ggtatcgggg
     3241 cgtggacgta tcacccctat tggtgtatga ccagcgggct gcctaccgct atttggaggc
     3301 tatcgcggct caagtggata tcccaacggt ggaggcatcg ctttcggtaa atggcactga
     3361 tgtggacgtg cgacctggcc aggtggggcg cagtgtagac atcgaagcta ccctggcacc
     3421 cctagaaggt cagttgcgca gcatgagtga tgggctgatc ccggtggtga tggtggagac
     3481 cccgcctatc atcctagatg caagcgccca agcggagatc gcccgcaaaa tcctcagcgc
     3541 gccgctcaac ttgatcgtgc ctgatgccgc cgaaggcgat cctggctcgt ggactttcga
     3601 ccagcaatct attgcccaga tgctgagcat cgagcgcgtt gaaacgcccg agggaatgcg
     3661 ttaccaggtg ggcctgagcg cagagaacct gcgcggcttt ctgcaaggcg tcagcgctga
     3721 gctggatcgc cagcccgcaa acgctcgttt tatctttaat gacgattcgc gccagctgga
     3781 ggtgatccaa tctgcagtga ttgggcgctc tctaaacgtg gatgcctcga tccaggagat
     3841 caaccagaag gcagtgcaag gcgagcacaa tatcccgctg gtcttcgatt acacccagcc
     3901 ggcggtgggc aacgatgcta ccgccgagca gttgggcatc acggagctga ccagcgtgca
     3961 gaactcgtat ttttacggct ccagctcgtc acgcatccag aatatccaga ccgcagccgc
     4021 tcgtttccat ggcttgctgg taccgccagg cgcgaccttc tcgatggccg atgccctggg
     4081 ggatgtgagc ctggacaatg gttacgccga ggcgctgatc attttcggca accgcaccat
     4141 caagggcgtc ggcggtgggg tttgccaggt gagcaccacg ttgttccgca cggctttctt
     4201 cgggggttac ccggttgttg agcggtaccc acacgcctat cgcgtctcgt attatgagct
     4261 aacccgcagc gggagcgtgg ataccgacct ggcaggattg gacgctaccg tgtttgtgcc
     4321 agtggtggac tttaagttca ccaacgacac gcccaactgg ttgctgatgg agacttatgt
     4381 caagccagag gcgcgtgtct tgacctggaa gttctactct acatccgacg ggcgcaccgt
     4441 cgattgggat tccaccgggt tggagaatgt tgaggagcca cccgagccgt tgtaccagga
     4501 gaacgaagaa ctggcgcgcg gcgagattcg ccaggtggat tgggaggcgg ctggtgctga
     4561 cgtcgtcgta taccgtaccg tccatcgcga cggtcaagta tacttacagg acgagttcgt
     4621 cactcattat atgccctggc gggcagttta cgaatatggc cccgggacga agattcccaa
     4681 ggataaacga aacaatgatg aggattgagg ctagttctca ccctcttaca cccgatctct
     4741 tctccatgtg tggggaagac aaattctttc cctgagtggg catacaggag cgaatcatgg
     4801 ttagccaaga actgctagaa attctgcgct gtcccaattg cgtacgtgaa aaacccggtt
     4861 tgctggagta ctataaagag gcctggttaa tttgccagga ttgcgggcgg aaatacccca
     4921 tccgcgaaga tatccccgtg atgctcattg acgaagggga taattggatc aacgtggcga
     4981 aggaagattt gcctgtcccg ccaccgggat aactcgtttt gtccgcctga agttaagctg
     5041 cgggcgaacc tatttgggga ccagagccgt ttttttatga tgacactgga cgagctttta
     5101 cttgagctaa ccagcgggga tgatgagcga gccgagggag cggtcaggag tctttctggc
     5161 tatggagaag ctgccctgga tgcattgcgc tccatgttag cctccccgga tgtcgatagg
     5221 cgctggtggg cggtgcgcgc actggcagaa atccctggcg gggcagctat tcctttcata
     5281 atccaagcgc tcagcgatga agacccggct gtacgccagt gcgctgccct gggtttgtgc
     5341 cagcagcccg atataccaga tgatagtgtg gcagaggtag tagtgccagc cttgatcgag
     5401 gcaatggctt cgaatgacgc cttgcttgcc aggctggcag ctaacgctct gacgtgtatt
     5461 ggcgcagcgg ctgttccagc cctgcttgac ctgctggcga actcacctga ccccgtgcgc
     5521 ttagaggctg cccgggcgct ggctttgatc ggcgacacgc gcgctatccc ggcgctcttt
     5581 gaagccttgg aagacgactc tgccctgatg gaatactggg ctaatgaagg cctggaaaga
     5641 atgggggtag ggatggtgtt ctttcaacca gagtaattct tcggagcatc tcccaagtta
     5701 gttcatcggt gcgcaggttt gtgtgaaagg cgcaccagcg ccgggtcgtc aaccactccg
     5761 ccccctctca cggtggcgcc aatgacaata cccgcactga taatgaccag gttttttatg
     5821 atgtactggc cctccagagt gggcgcataa gggagatgca gaaagatttc ttgggggaat
     5881 aagaagaccg gcgtgatggt gcccatcatt tgcatgaata gcaaaaacag ggtagcccgc
     5941 atccagatgc ctgagatcag gcctaacccg atgatacatt cccaagccgc caggatcggt
     6001 agtgacacct tggcagttat caagccgaag gtcagctgtt caatggtgcg cgctgccagg
     6061 tcttgagccg gactgagacc agggaagaat ttcagcacgc caaaccagaa gaagacgatt
     6121 cccagactga tccgcagaag caggacgcca tagcgggcca tccaatgggt gatacgctgg
     6181 tccacacgca gataccagcc atacaatgtt ttcatcgttc ttttatcctt tccacgccca
     6241 gatccaccac aaggacatca tcacgaaacc gatggcgaag cgcacgatga aggacatacc
     6301 ccaacctgca gccatgccgc gcagcgcccc ccaggctttc tccaggttgc ctgtgcgctg
     6361 gtactcgagc agcagaactg ccagcggtgc ggcgatcagc ccgccaaatg gcgggaaaat
     6421 caatgtaccc agtacgccag ccaccagggc gatagcgatg gtcttccagg aagcgcctcc
     6481 tttacgggcc cctgctccca agaagacatt atccgctagc gtcccggcca gcatcagaat
     6541 ggtgatgatg acgaataaaa cgatccccag cgtgccaaat ccggagacca caccgtatcc
     6601 caaggctgct aaccagatta tgactgtgcc aggaaagatg ggcacgatca agccgaataa
     6661 ccctaccagc atgatgacct gggtcagggc gaagatggaa gcgttgatcc agtcaggcat
     6721 ggtgtatcct catgcggcgc gaatcacgtc aacgccaccc atccaggggc gcagcacttc
     6781 aggcaccacc accgaaccgt catcctgttg atagttctcc aacacgccga tcagggtgcg
     6841 gggcattccc aaccccgagc cgttcagggt gtgcacaaag cgcgggcgac cgacatctgc
     6901 cgggcggtaa cggatattgg cgcgccgcgc ctggaattcg gtcacattcg agaccgacga
     6961 gacctccagc cattccccgc agcccggtgc ccacacctcg atgtcgtagg taatggttga
     7021 accgaagccc aggtcaccgg tgcagagctg cttgacgcga taaggcaagc ccagctgggc
     7081 gcaggtctgc tcagcgtcgg cgaccatctt ctcgaaggcg gctggcgagt cttcgggctt
     7141 gcagtagatg tacatctcta ccttgtcgaa ctggtggccg cgcttgatgc cgcgcacatc
     7201 gcgcccggca ctcatcttct cgcggcggaa gcagggcgtg taggcggtgt aatagcgcgg
     7261 cagggcgctt tcgtccagga tttcatccat gtgcaagccg gtgaggggca cctcggcggt
     7321 gggcaccatc cacagatctt cctcgtgatc ccggtacagg ttatcctgaa acttggggag
     7381 ctgccctgcg ccgaagaggg tctgcccttt gaccatgaag ggcgggtatt tctcggtgta
     7441 accctggcgg gagtgcaggt cgagcatcca ggcgatcagg gcgcgctgca ggcgagcccc
     7501 ggccccgctc agcacgtaga agcgcgagcc ggtgattttg accccctgtt cgaagtcgat
     7561 gatccccagg gctgggccga gttcccaatg tggtttgggt tcgaatgaat acttggggag
     7621 ctcgccaacc gtcttgatga gcacgttttc gttctcatct ttgcctaacg gggtggatgg
     7681 atcggggatg ttggggatca gagccatgac gccgtcgagc tggccttcca cctcccgcag
     7741 gctctcgtcg agtgcggtaa tacgatcgcc aaccaggcgc atggcttcga ttttggcctg
     7801 gcgctcggtg gggtccttca tacgcccgat ctctttggag gcggcgttgc gctctgcctt
     7861 cagggtttca acttcctgga tcaatagacg gcgcttctcg tccagctcca aggcttggtc
     7921 caccagcgtg ctctccatct ggcggacggc cattgcctgg cggacgcggt cggttgcttc
     7981 gcgaataagg ttgatatcga tcatgagtgc acctccgagt agctttcagt ggtcagccat
     8041 cagcgtgtag ctaaacgctg gaatgaaaac acccctctcc catgcaggac gagaggggcg
     8101 ctcgtggtgc cacctgcttt cgccgtttga aacggcctct gactcgcgct aacgggcgaa
     8161 cccgcgcccc cttacggcga ggaacctcgc ccctacgggg gggactggcc ctagcgggcc
     8221 ggcggagtgg atttcgccga ttatctgctg cctcgcaccc accggcagct ctctgggatg
     8281 ggaagcggct acttggctcc gcgctggtcc ttccggactg agatgccccg attataccca
     8341 tgaataaggg gatgtcaagg cgcggcgcaa aatgcttggt aaaatagtaa tctattcggg
     8401 catataactt gcagcacggt gatcgttatg ctccgccgga ttgaactcat ccatcctgct
     8461 gaaaaaacta ccctaccaga tgactccatc ggatttttcc ctgcgattaa gctgggtctg
     8521 atgggatcta ttaatccaaa gattgtcgag gcttggagtg ataaactgcg cggccctgga
     8581 aaacggcaga gtggcatccg gcgtaacgca cgcttttact tcaccgagct gggttggaag
     8641 gaagtaggtc gcaaggtggt gaaagcctgc cagcaagtgg ggcaggagta ccgcgtgatc
     8701 aaggtcaaag agggtacggt gaacgtggtc tggcgcgatc gccactatgg actggaggtg
     8761 gctgcacagc ccaggaaacg caggcacaac cggcggtgat tggatggatg agaagatcga
     8821 aattcgttcg ttagacgagg gtgacgccga ggttttccgg gctctccacc tgcgggcgtt
     8881 ggatgagctt cctgaggcct tccttgcggt gtacgaggag gaattatgcg agccgctgga
     8941 gcgcgtggca gaacgcctgc gaggttcaaa accggagaat ttctacctgg gagtttttgt
     9001 taacgggcac ctggtgggaa tggtacactt tgagcgcccg gggagtataa aaatccgcca
     9061 cagggcatac ctcagcggga tgtatgtcgc gccggagagg cgcgggcaag ggcttggtaa
     9121 ggccttgctg gatcgagcac tagcccacgc tcgcagcttg ccggacttgg aagaggtggg
     9181 cttgtgggtc atcattggca atcagcaggc ggaagcgctg taccaatcgg cggggttcga
     9241 gaccttttgt atcgagccgc ggtcgatcaa aatagacgac cggtattatg atggtgccgg
     9301 catgatcatg aggttgagat gagcatggat tttataaaag aaaacgccga ttctttggcc
     9361 aggctgaagg ccttcaccga tcgagtcagc ctgtcgcaac tggaacagcg cctggagagc
     9421 ggctggacgg tagccgccac actggcgcac atggcctttt gggatcagcg cgcctccttg
     9481 ctggtgcgcc gctggcggga gaacggtgtg gactcatcac cggtggatgt ggatgtgatc
     9541 aatgacgctt ccaaagcgct ctgccatgct ctccttccaa agcagacaat ccaactggtg
     9601 ttggagtcgg ccgaagctat tgatcgcgag atcgagggca tctcccctgc cctggcggag
     9661 gagatcgaag cgggcgccac ccagttccac ctcagccggg caaatcaccg agattaccac
     9721 ctggatgaga tcgaagcggc gttggaatag cacaattaag gctctaatac tctttccacg
     9781 caagttgttt tcaatatgaa caaatgtgct tacctatgca caaggcttga aagttgacgg
     9841 cgggaagtta tttcgggctt ctcgctggag taacccaccg gtaagatggc gaccgggcgc
     9901 aagtcttccc atacaccaat cacccggcgc accccgtctt cgtcgaaagc gcccacccag
     9961 acgctcccta aacccagggc agccgctgcc agatgggcaa aggcacaggc gatggtggcg
    10021 tcttgaatgc agtacagggt tgtgccgcgc cgtccatacc tcacggaaga acgcttcggg
    10081 ctggcacaga aaaccagcgc cacgggggct tgggcgatga acgtctgatc cagggcagcc
    10141 catgccagcg cttgcaggcg ggcggggtct gtcaccacgt aaatctcgta ggcttgcagg
    10201 ttgccagccg aaggggcgcg gttggctgcc tgcaagatgg cgtgcaactt ctcatcctcg
    10261 acgggttgcg aagtgtacga gcggatagaa tgccgttttt ctagcagttc gaaatagtcc
    10321 attgtcattt accttcactg tttggcttca ttggatcagg gcgtgcccgg ttgggatcag
    10381 gtaaagcggc tggtggtctg gaggcagctc gagcacctgc tgaacctgat catcatagaa
    10441 agctccaatt ggcaccgcgc cgaggtccag ggccaccgcc tggagcagga tgttttgggc
    10501 ggcgtgtccg gcctccaggt ggacatagcg agggctgcgt tcttcgccgt acttttttac
    10561 cgtccgggca tatactgcag cgattacaat cacagctggc gcttcgcgca cagccccctg
    10621 ttttaaggcg gcttcataca gcgcggcgcg cgcatcaccc tggatttgaa cgctcaggca
    10681 atgatcttgt ggcaggtcgt gatagactgc ctgctgcgtg acggcataga cctccagcgg
    10741 gtaaagcgcg cctgccgatg gggcgctgcg caagccggcg gggtgggtga tgccttgtgc
    10801 agcccacaat aactgtccta tctgttccaa cgtcagtgga tcgtcggaga actggcgcaa
    10861 agagcgtcg
//
LOCUS       DBNHOH010000196         2755 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_267191, whole genome shotgun sequence.
ACCESSION   DBNHOH010000196 DBNHOH010000000
VERSION     DBNHOH010000196.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_267191"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            304..1353
                     /locus_tag="AC16V7_03530"
     CDS             304..1353
                     /locus_tag="AC16V7_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR6256223.1"
                     /translation="MKKSLLILSILLVVALVASCAPQAAPTEEAAPPEEGAPPEGEEM
                     VFASVVKSIAFNWFLRLETGVLDFGEDYGVKAFMEGPSQVDSAAQVAIIEDLIAQGVD
                     AICNVPYGVPENEPAQKKAMDAGIIVIGHEAATAKAGTLNYDVEAFDNCAYGEEMMKE
                     VATRMGESGQYIQFVGSLTNASHNEWQDCAKAYADANYPNLEFVAKYESKEDQEVAYN
                     TMKDVLKTYPDVKGVLGSAAGDVVGAGRAIEEAGLQDAVAVVGTSIPSYAGELLKTGA
                     VDLAMAWDPATAGYACNVVAYKLIKGEAITDGMDLGVPGYEKIVIRKGENGVDVIYGA
                     AWIKMDASNMDDYPF"
     gene            1436..>2755
                     /locus_tag="AC16V7_03535"
     CDS             1436..>2755
                     /locus_tag="AC16V7_03535"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR6256224.1"
                     /translation="MTEEILRVENVSKRFGGVIALDNVSLSIHKGETCCLVGENGSGK
                     STMIKIISGVYTPDEGDIYINGNHYKKLTPIGSINEGIQVIYQDFSLFPNLTVAENIA
                     TNEQLASGKQLVNWSEINRIAKEGLAKINISLPLDVIVSTLSTADRQLIAIIKALLAN
                     ARIIIMDEPTTALTKKEVASLFKLIDELKAQGISTLFVSHKLTEVTEIADRTVIFRNG
                     KKVMDQDAKGLDVQTMEFYMTGRHIDTSAIQFGEVQEAAEPLFRVENLHLSHGFFDLS
                     FELKHNEVLGITGLLGSGRTELALSLFGELPADSGKIFIEGKEVKIKGITDAVRYGIG
                     YVPDDRIREGLFLEQSISNNIVVSVIDRLINKMRLLNMKGKAEESKRWIKQLEVKTPS
                     GELPAKSLSGGNQQRLVLAKWLASKPKILILNGPTVGVDVGSKAEIHE"
BASE COUNT          745 a          613 c          750 g          647 t
ORIGIN      
        1 caaagagatc accgctgtgg tcgattcgag caaattcgga caagttgcct gaactgccat
       61 cgcgcccctg aaggctgtaa atcgcatcat tacggacaac ggacttgagc cgaaaatggc
      121 tgatcgaatc cgtgaaatgg gaattgaaat agtcctggta taacggcaaa ttattggttg
      181 aatcgattcc taaatgagga caatacaaga cctggttgct gaaaggaggt gaatagcaaa
      241 aattcctggc aaaaataatc tatagcataa accaatcgaa atcaatcttg caaggagaga
      301 aaaatgaaaa agagcctgct gatcttatcc attctgttgg tggttgccct ggtggcatcc
      361 tgcgcacctc aagcggctcc gacagaagaa gctgcacccc ccgaagaagg cgctccacct
      421 gaaggagagg agatggtctt cgcctcagtg gtgaagtcga tcgcttttaa ctggttctta
      481 cgcctggaga ctggggtatt agatttcggg gaagactatg gggttaaggc ctttatggaa
      541 gggccctccc aggttgactc ggctgcccag gttgccatca tcgaggacct gattgctcag
      601 ggagtggatg cgatctgcaa cgtcccttac ggtgtgccag agaacgaacc agcccagaag
      661 aaagcaatgg acgcgggcat cattgtcatt gggcatgagg ctgctactgc caaagcaggc
      721 accctcaact acgatgttga agccttcgac aactgcgcct acggcgaaga aatgatgaaa
      781 gaagtggcga cccgcatggg tgagtccggt cagtatatcc agtttgtcgg ctcgctgacc
      841 aatgcctccc acaatgaatg gcaggattgt gccaaagctt atgccgatgc caactatcca
      901 aacctcgagt tcgtcgccaa gtacgaatcc aaagaagacc aggaagtggc ctacaacacc
      961 atgaaagatg tcctcaagac ctatccggat gtcaaaggtg tcctgggttc cgcagcgggc
     1021 gacgtggtgg gtgctggtcg tgccattgag gaagctggcc tgcaggatgc tgttgccgtg
     1081 gttggcacga gcattccttc ctacgccggc gagttgctca aaaccggcgc agtcgatttg
     1141 gctatggctt gggatcccgc cactgcagga tatgcctgca acgtggtggc ctacaaattg
     1201 attaagggcg aggcgataac agatggcatg gacctcggtg tgcccggata tgagaagatt
     1261 gttatcagga agggcgagaa cggtgtggat gtgatctatg gggcggcgtg gatcaagatg
     1321 gacgccagca acatggatga ttatccattc taagccaata taatgaacta gtgggcggct
     1381 gaatgtctca gccgcccact cttacccatt ctataataaa taaggtgcat agttcatgac
     1441 agaggaaatt ctgcgtgtcg aaaatgtcag caagcgattt ggtggggtaa tcgcgctgga
     1501 caatgtgagt ttatctatcc ataagggtga aacttgctgc ttggtggggg agaacggctc
     1561 cggaaagtcc acgatgatca agatcatttc gggggtttat acaccggatg aaggtgatat
     1621 ttatatcaat ggtaatcatt ataaaaaatt aaccccgatc ggatcgatta atgagggcat
     1681 ccaggttatt tatcaggatt tttctctttt ccccaacctc accgtagccg aaaatatcgc
     1741 taccaatgaa caactcgcaa gtggcaagca actggtcaat tggtcggaga ttaaccgcat
     1801 tgcgaaggag ggcttggcga aaatcaatat ttcactgccg ctggatgtca tcgtcagtac
     1861 attgtccaca gctgatcgcc agttgattgc cattatcaaa gcgcttttgg ccaatgctcg
     1921 tatcatcatt atggatgaac cgactactgc cttgaccaaa aaagaagtgg cttcattgtt
     1981 taaattgatt gatgagctaa aagcgcaggg aatctcgacg ctttttgtta gccataaatt
     2041 gaccgaggtg accgaaatcg ccgaccggac cgtgatcttt cgcaatggca aaaaggtgat
     2101 ggaccaggat gccaaagggc tggacgtcca gacgatggaa ttttatatga ccggtcgcca
     2161 cattgatacc agcgcaatcc aatttgggga agtgcaggaa gcggctgaac ccctgtttcg
     2221 ggttgaaaac ctacatctct cgcacggttt cttcgatctc tctttcgaac tcaaacataa
     2281 tgaggtatta ggtattacgg gtttgctggg atcaggccgg acagagctgg cattatcctt
     2341 atttggggag ttgccggcag attcagggaa gatcttcatc gaaggtaaag aggtcaagat
     2401 caagggcatt acggatgctg tcagatatgg cattggttac gtgccagatg atcgcatccg
     2461 tgagggcttg ttcttggagc aatctatcag caacaatatc gtagtcagtg ttatcgatcg
     2521 gctgattaat aaaatgcgac tcctcaatat gaaaggcaaa gccgaagaat cgaagcgctg
     2581 gatcaaacag ctcgaggtga agacaccttc aggcgagctg cctgccaaga gcctttctgg
     2641 tggcaatcag cagcgattgg tgcttgccaa gtggctggca agcaagccga agattctgat
     2701 cttaaatggc ccaaccgtag gagtggatgt tggttcgaaa gcggaaattc acgag
//
LOCUS       DBNHOH010000197         6314 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1165317, whole genome shotgun sequence.
ACCESSION   DBNHOH010000197 DBNHOH010000000
VERSION     DBNHOH010000197.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6314)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6314)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6314
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1165317"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1882
                     /locus_tag="AC16V7_03540"
     CDS             <1..1882
                     /locus_tag="AC16V7_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009361546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6256225.1"
                     /translation="QFLIEHARQTPNKYAMRERNYGIWQAITWGQYLEHVKHFCLGMV
                     SLGLQPEEAIAIIGDNRPEWVIAELAAQSAGAKSIGLYQDSGVKEIIYIILHADVSFI
                     IVEDQEQVDKILEMWSELRGVRKVIYYDPKGLRNYTEDFLISFQDVEQLGQLYDRDHP
                     GWFEERVAHGKASDIAILSTTSGTTGDPKLAALTHESMVSMGHNLMKVDPMAPQDEFV
                     SFLPLAWIGEQMMVISCGLKVGFTVNFPEEPETVQHDLREIGPQVMFSPPRIWENLVS
                     QVQVKIEDSSLLKKKLYNWAMPIGYQMADIRFRKETPSLGLRWKYKLADWLIFQEIKD
                     HLGLRRLKRAYTGGAALGPDVFRFFHALGVNLKQIYGQTEINGIAVVHRDGDIKFQTV
                     GTPIPETEIKIDENGEILMRSPAVMTSYYRNPDATAGALKDGWLHSGDAGYFDEDGHL
                     IVIDRAKDVMTLHDGTKFSPQFIENKLKFSPYVREAVVFGGDWPFVSAMINIDFANVG
                     KWAENHQIAYTTYTDLSQKVQVYELIRQQVERANADLPPAARIQRFLLLHKELDADDA
                     ELTRTRKVRRNLIAQRYDDIISALYSQSSHLDVETTITYQDGRTAVLQTRLKIESMNG
                     GG"
     gene            1909..2781
                     /locus_tag="AC16V7_03545"
     CDS             1909..2781
                     /locus_tag="AC16V7_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6256226.1"
                     /translation="MELFIQLTLTGLTNGAILALAALGFVLIYKSSDVINFAQGEFLL
                     VGAYITFAMIAQFGLVWPLGMILTMFLAVVMGVIVERLVLRPMIGEPIISIIMVTIGL
                     SSLLRAIVSGIWGNLPRAFPPFIPSQTVKILSAVVGADRLWAFVIALILLGLFSLFFR
                     RSKEGIAMRAVADDQQAALSMGISVKKIFAWAWSIAAATAAIGGALVANIVGVSGELS
                     GFGLRVFPVVILGGLDSIPGAIVGGIIIGLLEAYTGGYIGQGLNTVIPFVLLIFILMV
                     RPYGLFGQEIIERV"
     gene            2944..3987
                     /locus_tag="AC16V7_03550"
     CDS             2944..3987
                     /locus_tag="AC16V7_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6256227.1"
                     /translation="MESGIFHATYQSDMALRRTHAQKIRIAIFLIFMGVFPFFASRYT
                     LTLANQVGIATIGAIGLNILVGYTGQISLGQGGFLAVGAYTAGILTARLGMPWYVSTV
                     LACLVTALIGAIFGIPSLRLKGLYLAIATLAAQEIILWLVTHWDALTGGVDALVVPNP
                     TFFGLQLNTDFRFYFVIWGLAGLTALATINLFRSHYGRAFIAIRDQDIAAEVMGVNIF
                     RYKLLAFAISSFFVGLAGALTAHYRSIVTWERFTIDVSVLYLAMIIIGGLGSVPGSFF
                     GATFMTLLPAVLTNLGRALKSTFPIIETVIPFIQLAAFGLVIILFLIFEPEGLNKMWR
                     NIKDYFRLWPFSY"
     gene            4085..5296
                     /locus_tag="AC16V7_03555"
     CDS             4085..5296
                     /locus_tag="AC16V7_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6256228.1"
                     /translation="MFQRNRTIQTWMVVFILATLLLSACGGGGGAGETSPVKVGAIFD
                     LTGATADVGTPYSEGLKAYVEWRNANGGINGHPIELISADYAYKVDVAEQLYTQYVQE
                     GVVAFMGWGTGDTEALRSKIASDKIPFMSASYSANLLDMAQAPYNFLIGTSYSDQALI
                     ALRWAAEDWEASGGAGPAKVAFIHHDSPFGKSPIPDAEAYAQAHGIEFVSLPMPGGVT
                     DYVAELAQVQQFGAGYIVVHTVSSPAAVLVKDIVSQGMKDKVKVINLNWCSDEIFINL
                     AGDAAEGVIGAIPFTPPSSPVPGHDDADKWLKDKGSSLAEKGLHFTQGWWTMAVMSEG
                     IKKVLDEGKEVNGENIKAALEGIQNYDTKGITAPISFSSSSHKGNSALRLFVVEGGQW
                     KQRSDFISAVP"
     gene            5369..6160
                     /locus_tag="AC16V7_03560"
     CDS             5369..6160
                     /locus_tag="AC16V7_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6256229.1"
                     /translation="MLTLNNVEVIYNEVILVLKGMSMDVPQGKIVGLLGSNGAGKSTT
                     LKAISGLLKAEDGEVTDGSIEFIGQPVHHRDAAEIVRMGIFQIMEGRRVFEHLTAEEN
                     LVAGSYTRQDGKVRDDIDMVYQYFPRLKERRNQTAGYLSGGEQQMLAIGRALMARPKL
                     MMLDEPSLGLAPMLVQEIFGIIKRINSEHGTTILLVEQNANLTLGVADYAYIMENGRI
                     VLDGYPADLRENADVREFYLGLTEVGQRKSYRDVKHYKRRKRWLS"
BASE COUNT         1492 a         1431 c         1725 g         1666 t
ORIGIN      
        1 ccaattcctc atcgaacatg ctcgccagac tcccaataag tatgcaatgc gggagcgaaa
       61 ctacggcatt tggcaggcta tcacttgggg acagtacctg gagcatgtca agcacttctg
      121 tcttggaatg gttagtttgg gcctgcagcc cgaggaggcg attgccatta ttggggataa
      181 ccgcccggag tgggttattg ctgaactggc agcacagtct gcgggggcga aatcaatcgg
      241 tctgtatcaa gattcgggtg ttaaagagat tatttacatc atcttgcatg ctgatgtgtc
      301 attcataatt gtagaagatc aggagcaagt agacaaaatt ctggaaatgt ggtctgagtt
      361 gcggggtgtg cgcaaagtta tttattatga cccgaaggga ttgcgcaact ataccgagga
      421 tttcctgatc tcctttcagg atgttgaaca attgggtcag ctttacgata gagaccaccc
      481 cggctggttt gaagagcgag tcgctcatgg aaaagccagt gatatagcaa tcttatctac
      541 gacatcaggt acaactggag atccaaagct ggctgcacta acccatgaga gcatggtcag
      601 catggggcac aacctaatga aagtggaccc catggcgcct caggatgagt tcgtctcttt
      661 tctgcctctg gcttggatcg gcgagcagat gatggtcatc tcttgcggct tgaaagtggg
      721 tttcacggtc aatttccctg aagaaccgga gacagtgcaa catgatctgc gcgaaattgg
      781 tccccaggtc atgttcagcc caccacgcat ttgggagaat ttggtttccc aggtacaagt
      841 aaagattgaa gatagctctt tactaaagaa aaaactgtac aactgggcga tgccaatcgg
      901 ttatcagatg gcggacattc gttttcgtaa agagacgccg tctctggggt tgcggtggaa
      961 atacaagctt gcagattggt tgatatttca agaaattaag gatcatctgg gtttgcgtcg
     1021 cttgaaacgc gcttatacgg gtggggcggc gcttggcccc gatgtatttc gcttttttca
     1081 cgccctgggc gtgaatttaa aacaaattta cgggcaaacg gagatcaacg gcattgcggt
     1141 tgtccaccgc gatggtgaca tcaaattcca gactgttggc actccgatcc ccgaaaccga
     1201 gatcaaaatc gatgaaaatg gcgaaatatt gatgcgttct ccagctgtga tgactagcta
     1261 ctatcgcaat cccgatgcga ccgcaggtgc gctgaaggat ggctggttgc attctggaga
     1321 cgcaggctat tttgatgaag atggtcacct gattgtcatc gatcgtgcca aagacgtaat
     1381 gactctacat gatggcacaa agttcagccc ccaattcatt gagaataaac ttaagttcag
     1441 cccttatgtc cgagaggcgg ttgtattcgg aggcgactgg ccttttgtca gcgcaatgat
     1501 caatattgat tttgcaaacg taggcaagtg ggctgaaaat catcagatcg cttataccac
     1561 atacacggat ctctcacaaa aagtccaggt gtacgaactc atccgccagc aggtagagcg
     1621 cgccaacgca gatttgccgc cggctgctcg tatccagcgt tttctcttgc ttcataaaga
     1681 attagatgct gatgatgccg agcttactcg tacccgaaaa gtaagacgga atctcattgc
     1741 ccagcgctat gacgatatca tctccgcatt gtacagccaa tcctcgcacc tggatgtgga
     1801 aacgacgata acgtatcaag acggtcgcac agctgtcttg cagactcgcc tgaagatcga
     1861 gagcatgaat ggtggcgggt aacgggtgac cgcatcaacg gaaatattat ggaactcttt
     1921 atccaactaa ctctgactgg tttgacgaat ggtgccatcc tggctttggc cgctttggga
     1981 tttgtgctga tctataaatc cagcgatgtg atcaatttcg cccagggtga attcctgctg
     2041 gtgggcgcgt acatcacatt tgccatgatt gcccaattcg gcctggtatg gccattaggc
     2101 atgatcttaa ccatgtttct agcggtagtt atgggggtca ttgtggagcg tttggttttg
     2161 cgccccatga tcggtgaacc aatcatctcc attatcatgg tgacgattgg attaagcagc
     2221 ttgctgaggg ctattgtcag cggcatttgg ggtaacctcc ccagggcatt tccaccgttt
     2281 atccccagcc aaacggtcaa aattctgtcc gctgtggtgg gtgctgatcg cttgtgggcg
     2341 tttgtcatcg cgttgatctt gttaggtttg ttctctctgt ttttccgtcg ttcaaaggaa
     2401 gggattgcca tgcgggctgt tgctgatgat cagcaagcag cactcagcat gggaattagt
     2461 gttaaaaaaa tatttgcctg ggcttggtca atcgcagcag ccacggcagc tatcggggga
     2521 gcgctagtcg ccaacattgt tggtgtaagc ggtgaactca gtggttttgg tctgcgcgta
     2581 ttcccggtcg tgatcttggg cggtttggat tcaattccag gtgctatagt gggagggatc
     2641 attatcggct tgctggaggc atacactggc ggctatattg gtcaaggtct taatactgtg
     2701 attccatttg tgttgcttat ctttatcctg atggtgcgcc cctacgggct atttggccag
     2761 gagatcatcg aacgtgttta actattatag gacttacgca gttgaagagg gaagaggagg
     2821 gtgtgggctg gcgcagcccg cccaaactcc cccatcgcct ctcgaactgc gtaacttatg
     2881 tattatttag ttacggaacc attagctttg tgtgaaacgc tttggtattg gagtctttga
     2941 gctatggaat caggtatttt tcacgctaca taccagtcgg atatggctct tcggcgcacg
     3001 catgcgcaga aaatacgtat tgcgattttt ttgattttta tgggcgtatt tcctttcttt
     3061 gcgagtcgct ataccctgac gctggcgaac caggtcggaa tcgctacgat tggtgcgata
     3121 gggttaaaca ttctcgtagg atataccggt cagatcagcc tggggcaggg gggatttttg
     3181 gcggtaggtg catacactgc tggcatcttg actgcccgtt tgggcatgcc ttggtacgta
     3241 tcgaccgttc tggcttgcct ggtgacggct ttaataggag caatatttgg catcccatct
     3301 ctacgcctga aaggcctcta tctggcgatt gctaccctgg ctgcacagga gatcatcctt
     3361 tggctggtca ctcattggga tgctctgaca gggggtgtcg atgcgctggt tgttccaaat
     3421 ccgacctttt ttggtctaca gttgaacacg gacttccgtt tctattttgt catttggggt
     3481 ctggctggcc taaccgcgct ggcgacaatc aatttattcc gctcccatta tggacgcgcg
     3541 tttatcgcta tccgggacca ggatattgct gccgaagtaa tgggcgtaaa catctttcga
     3601 tacaagctgc tggcatttgc gatctcatcg ttctttgtcg gcctggcagg tgcgctcaca
     3661 gcccattatc gtagcattgt gacctgggag cgatttacaa ttgatgtgtc ggtgctttat
     3721 ctggctatga tcattattgg tggattgggc tcagtaccag gctcgttctt tggagcaacc
     3781 ttcatgaccc ttctacccgc ggtgctgacc aacctggggc gcgcccttaa atctacgttc
     3841 cccattattg agacggtgat cccattcatt caactggccg catttgggtt ggtgatcatc
     3901 ctgtttttaa tttttgaacc agaggggttg aataaaatgt ggcgtaatat caaggattat
     3961 tttcgtctgt ggccgttcag ctattagctc aatgaagaac agaggaggtg ctctttgcag
     4021 gatgccaaaa tattcgtttc aatattagcc aatccataat ccatcaatca gaggaggaga
     4081 cattatgttc caaagaaata gaactattca aacctggatg gttgttttca tcctggcaac
     4141 actgctgctc tcggcatgtg gtggcggagg aggcgcagga gaaacgagcc cggtcaaggt
     4201 cggcgcaata tttgatctca ccggcgcgac ggcagatgta ggtaccccat attccgaagg
     4261 gcttaaagcc tatgtggaat ggcgcaatgc caacgggggt atcaatgggc atccgatcga
     4321 gttgatctcg gctgactatg catacaaggt cgatgttgcc gagcagttat atacccaata
     4381 tgtccaggaa ggggttgttg ccttcatggg ttggggtact ggcgataccg aagctctgcg
     4441 atcaaaaatc gcatcagata agatcccctt catgtcggct tcttactctg ccaacttgtt
     4501 ggatatggct caagccccct ataatttctt gattggtaca tcttactccg atcaagcgct
     4561 catcgcgttg cgctgggctg cagaggactg ggaagccagc ggtggtgcag gaccggctaa
     4621 ggtagccttt atccatcacg acagcccatt tgggaaatct ccaattcccg atgcagaggc
     4681 ttatgctcaa gcccatggta tcgagtttgt gagcctgccc atgcccggcg gtgtgacgga
     4741 ctatgtggct gagctagctc aagtacagca atttggagct ggttatattg ttgtccacac
     4801 agtatccagc ccggctgctg tgctggttaa ggatattgtc agccagggca tgaaggataa
     4861 agtcaaggtc atcaacctga actggtgttc agatgaaatt ttcatcaacc tggctgggga
     4921 tgcagctgag ggtgtcatcg gagcgattcc tttcaccccg ccaagctctc ccgtcccagg
     4981 gcacgatgat gcagacaaat ggttgaaaga taagggctcc agccttgcgg agaagggcct
     5041 gcactttaca caaggttggt ggacgatggc ggttatgtcc gagggtatca agaaagtctt
     5101 ggatgagggc aaagaagtca acggcgaaaa catcaaagcc gccctggaag gtatccaaaa
     5161 ctacgacacc aaggggatta ctgccccgat ttccttctcc tccagcagtc acaagggtaa
     5221 cagcgccctt cggctgtttg tggttgaagg tggtcaatgg aagcagagat ctgattttat
     5281 ctcagcggtc ccttaagaga tcgcccgtgg aggatggcag aggagtgtca ggtgagagcc
     5341 cctgccatcc tccaaaaatg gttgaaatat gctcacgctt aataacgtag aagtaattta
     5401 taacgaagtc atcctggttt taaaaggcat gtcgatggat gtgccgcaag ggaaaattgt
     5461 tgggctgctt ggttcgaatg gcgcgggaaa gtccaccacc ttgaaggcaa tctcaggctt
     5521 gttaaaggca gaggatggag aggtcacgga tggctcgatc gagttcatag gccaacctgt
     5581 tcatcatcgc gatgcagcgg agatcgtccg gatgggaatt ttccagatta tggaggggag
     5641 acgggtattc gagcatctca cggcggaaga aaatctggtc gcaggaagct acacccgcca
     5701 ggatggcaag gtgagagacg atatagacat ggtttaccaa tatttcccgc gcttaaaaga
     5761 acgcaggaat cagaccgcgg gctacctctc tggcggtgaa cagcagatgc tggcaattgg
     5821 ccgtgcgtta atggctcgac caaagctgat gatgttggat gagccatcct tggggcttgc
     5881 tccgatgcta gtccaggaga tttttggtat tatcaagcgt attaatagtg agcatgggac
     5941 gactatcctg ttggtcgagc aaaacgccaa tctgacttta ggtgtagcag attacgccta
     6001 cattatggag aatgggcgta tcgtcctgga tggttatcca gccgacttgc gcgagaatgc
     6061 tgatgtgcgc gaattttatc tcggcttaac ggaggtcggt caacggaaat cctatagaga
     6121 cgtaaagcac tacaagcgtc gcaagcgctg gctatcgtga gaatggatct tccaaacctg
     6181 gttgcccact tagcagagaa tgcgcctggt tttgctgcgc gtatcaaggc ggcgggcctg
     6241 acactggacg atctgcgcaa tccaaaagca ttggatgcat tgcccgtcgt tcgcaaggat
     6301 gatttggtgg agtc
//
LOCUS       DBNHOH010000198         3632 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_139393, whole genome shotgun sequence.
ACCESSION   DBNHOH010000198 DBNHOH010000000
VERSION     DBNHOH010000198.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_139393"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="AC16V7_03565"
     CDS             <1..869
                     /locus_tag="AC16V7_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR6256230.1"
                     /translation="TYPEVISLPDGFRPEGIAVGKGHTFYTGSLGNGAIYRGDLRTGE
                     GELIFNGQAGRIAVGMSFDERSGYLFVAGGLTGQAFVFDGETGDEVAAYTLSSPGASF
                     INDVIVTRQAAYFTNSFEKVFYRVPLGPAGELPDDPEEIQSIPLGENFVQVPGAFVFN
                     SNGIEASEDGDMLVIVNTARGELYRVNPLYGHADLIDLGGMSVSNGDGLLFRGSDLYV
                     VRNQLNQIEVVKLDREWTSGEIVDSLTNSNLRVPTTAAAFGNSLYLVNARFGVTPTPD
                     TEYEAVRLSLKH"
     gene            complement(989..1759)
                     /locus_tag="AC16V7_03570"
     CDS             complement(989..1759)
                     /locus_tag="AC16V7_03570"
                     /inference="COORDINATES: protein motif:HMM:NF025687.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HIR6256231.1"
                     /translation="MSQETTSANPDRGTESLDLTWKGLYQIGGAAALICALMYLVTLG
                     VYVPANLVSPPPETVLEWFLVFEGSPITGLFFLGLADIIIMILWGPMTLALYIVLKEA
                     NRIWSLIAIPFVFVGMAVYLATNTAFSMLSLSQEYAVAATEVEKSVLLAAGQAMLAVS
                     RGTGLYSGMALAWLAGLILAIVMLQSKVFSKATAWAGILGWGLLVAGTPFGGHYTGTG
                     TPTAVQSAMVALQYIGGGLLSLVWYILVGLKLLKFGRS"
     gene            complement(1963..2808)
                     /locus_tag="AC16V7_03575"
     CDS             complement(1963..2808)
                     /locus_tag="AC16V7_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HIR6256232.1"
                     /translation="MKIYAIQTGTVVIRPNQVQGKGAGFVRLVNTFSDRRWTEPLPIY
                     VWVIEHPEGIIVIDTGETAKAALPGYFPSWHPYFKNVIIQVKQGQEVGEQLQTIGIHP
                     NDVRWVILTHLHTDHAGGLHHFPKSEILVVRRAFELATGFAGQVRGFLPHRWPSWFAP
                     KLIDLAPNSFSVFPKSFKLTSAGDVIIVPTSGHTEAHISVVLQDADFSYFFAGDASYS
                     EQLMLEQQIDGVAINYREASQTLERIRQFAYSTPCIYLPTHDPESGNRLATKRLVPLD
                     GSHPS"
     gene            complement(2955..3488)
                     /locus_tag="AC16V7_03580"
     CDS             complement(2955..3488)
                     /locus_tag="AC16V7_03580"
                     /inference="COORDINATES: protein motif:HMM:NF015438.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR6256233.1"
                     /translation="MHNSFNNGSVVAVCKKAEPGLPKDVVDVIQLLENFGVEGDYHAG
                     KLVRHRFLAKKYPNKLNLRQVLIIDKTIHAEIEAQGIHIRAGMMGENILVDGISIMAL
                     PVGTQVGIGEAILELTEVRNPCYQLNEMHPHLLKAVAKKVDGKVLRNAGMMARILKGG
                     MVQIGDPVIVYNKPAEL"
BASE COUNT          899 a          917 c          875 g          941 t
ORIGIN      
        1 tcacctaccc cgaggtgatt tcactaccag atggttttcg ccccgaaggg atcgcagtcg
       61 ggaaagggca taccttctac accggctccc tggggaatgg tgccatctac cgcggcgacc
      121 tgcgcacagg tgagggcgag ttgatcttca atgggcaagc cggaaggatc gcggttggaa
      181 tgtcctttga tgagcgctcc ggttatcttt tcgttgcggg cggactgact ggacaggctt
      241 ttgttttcga tggagagacc ggtgatgagg tagccgccta taccctctct tcaccgggcg
      301 ccagctttat caatgatgtc atcgtgactc gccaggctgc ttacttcacc aactccttcg
      361 agaaagtctt ctaccgggtg cctttgggtc ccgctggtga gcttcccgat gacccagaag
      421 agattcaatc gatacctttg ggtgaaaatt ttgtccaggt gcctggagcg tttgtcttca
      481 actccaacgg catcgaggca tccgaggatg gtgatatgct ggtaatcgtc aatacagcgc
      541 gaggtgagct atatcgggtg aatcctttat acggccatgc tgacctgatc gatcttggcg
      601 ggatgagtgt ctcaaatggc gatgggttgc ttttccgtgg gtcagatttg tatgtggtcc
      661 gtaaccagtt gaaccagatc gaggtggtta aattggatcg agaatggacc tccggagaga
      721 tcgttgactc gctcaccaat tctaatctgc gcgttccaac aacagctgca gctttcggga
      781 atagcctgta tttagtcaac gcccgctttg gcgtcacacc cacaccggat actgagtatg
      841 aagcggtgcg attatcgcta aagcattaac ctggaatatc ttgcagggaa tttattagcc
      901 acgcaccaga cctggtgcgt ggctaatttt tatctccatg caagggatat taggggctta
      961 cttttccctt cctgcctgtg cccacccttc aagatcgacc gaacttcaga agctttagcc
     1021 ctactaatat ataccacacc agcgaaagca aaccgccgcc gatatattgc aaggcgacca
     1081 ttgcgctctg aacggctgtt ggcgtgccag tgcccgtgta atgaccaccg aatggcgtgc
     1141 ccgccaccaa gagtccccat cccaggatgc ccgcccaggc ggttgctttg ctgaaaacct
     1201 tgctctgcag catgacaatc gcgaggatca gaccggcgag ccatgccagt gccattcctg
     1261 aatacaagcc tgttcccctg ctgacagcga gcatggcctg cccggctgcc aacaagaccg
     1321 atttctccac ttctgttgct gctacagcat actcctgact aagcgaaagc atggaaaagg
     1381 ctgtattggt cgcaaggtaa accgccatcc ccacaaagac aaatggtatg gcgatcaacg
     1441 accagattct gttagcctct ttgagcacaa tgtatagggc tagagtcatc ggaccccaga
     1501 gaatcatgat gatgatgtcg gccaacccga ggaaaaacag ccccgtgatc ggactgcctt
     1561 caaagaccag gaaccattcg agaacggttt cgggaggtgg gctgaccagg ttggcaggga
     1621 cgtagacccc caaggtaaca agatacatca aggcgcaaat cagggctgcc gctccgccta
     1681 tttggtaaag ccctttccat gtgaggtcca aggattccgt tccacgatct ggattagcgc
     1741 ttgttgtctc ctgtgacatg gcttgctcct taatcttggc gaattttgaa gataatcctt
     1801 tcgatggaaa aaatgtaaaa ctcaaagcga ttcactgcta cagcgcctga caggttacgt
     1861 tacctatcgc tgattttaac ggatgggtca tcgacatatc tcttctagca agggaatgac
     1921 cacaagtcaa actgtgccga tggcagtcag gggctcattg tgttagcttg gatgcgaacc
     1981 atcaagcggc acaagccttt tagtcgcaag acgattgcct gactctgggt catgtgttgg
     2041 caaatagatg caaggtgttg aatatgcaaa ttgccgaatt cgttcgaggg tttggcttgc
     2101 ttcgcggtaa ttaatggcaa ctccgtcaat ctgttgctcc aacatcaatt gctcggagta
     2161 ggaggcatcg cctgcaaaga aataagagaa atcagcatct tgtaatacaa ctgagatatg
     2221 cgcctcagtg tgaccggaag ttggaactat gatcacatcc ccagcacttg ttagcttaaa
     2281 gcttttggga aatacactaa aagaattggg agcaagatct attaatttgg gggcaaacca
     2341 tgacggccaa cggtgtggca aaaagccgcg cacttgaccc gcaaagccag tggctaattc
     2401 aaaggctcgt ctgacaacaa gtatttctga ctttggaaaa tgatgtaacc caccagcgtg
     2461 gtcagtgtgg aggtgagtaa ggatgaccca gcgaacatca ttgggatgaa tacctatggt
     2521 ctgtaattgt tcgccgactt cttgtccttg tttcacttga atgattacgt tcttgaagta
     2581 aggatgccaa gatgggaaat aacctggcaa agcggctttg gcagtttcgc ctgtgtctat
     2641 gacaataatc ccttcaggat gttctataac ccagacgtaa ataggaagcg gctctgtcca
     2701 acgacggtca gagaatgtat tcactagcct gacaaagccc gccccttttc cctggacttg
     2761 atttggtcta attacgacag tgcctgtttg gattgcataa atcttcatgt attttccttt
     2821 tgaaacaaag aaccaaacct cgtaaatcgc accccctact ctcggtcgca gcccagcctt
     2881 gtgctggggg ggtagccggc ccaatattta tcgaacaaga agtcccccaa atattatcgc
     2941 ggaacaagca gatgtcacag ttcagcgggt ttgttataca caattactgg gtcgcctatc
     3001 tgtaccatgc ctccttttag aatgcgcgcc atcatccctg cattacgaag cacctttcca
     3061 tcgacttttt tcgctactgc tttgagcaga tgtgggtgca tctcattgag ttgataacat
     3121 ggattccgaa cctcagtcaa ttccaaaatc gcctcaccaa tccctacctg cgttcctacc
     3181 ggcagagcca taatagagat gccatctact aggatgttct cacccatcat ccccgctcta
     3241 atatggatgc cttgtgcttc tatttcagca tggatcgtct tatcaatgat cagtacctgg
     3301 cgtagattga gtttgtttgg atattttttc gccaggaagc gatgccgtac caacttccca
     3361 gcatggtaat caccttcgac accaaaattt tctaaaagct gtatgacatc gacgacatct
     3421 tttggcaatc caggctcagc ttttttgcaa acagcaacta ccgacccgtt attgaatgaa
     3481 ttatgcatta ggttgtcctc cacacctgga taacaacttt cgattccttt tacctgtcat
     3541 aatattaatg cagagcgcgt cctttggctt ggatatttga tgaaaccggt cgtgtttgcc
     3601 cgagcctcgt gtaattataa ccaatgaacc tt
//
LOCUS       DBNHOH010000199         2640 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_460542, whole genome shotgun sequence.
ACCESSION   DBNHOH010000199 DBNHOH010000000
VERSION     DBNHOH010000199.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_460542"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="AC16V7_03585"
     CDS             <1..479
                     /locus_tag="AC16V7_03585"
                     /inference="COORDINATES: protein motif:HMM:NF024581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR6256234.1"
                     /translation="CTERLPVIYDTLIGEPPANQVEPTTSARLYYLETLSPIERMDFW
                     LSHFDRCIRCYACRQVCPMCDCPTCLFERDDSLWVGPGIRLDEKRIYHLGRAYHLAGK
                     CVDCHECERVCPVNIPISLINSKLNLEMEKFFGYRAGEQAIPSPLLTILSGEERLP"
     gene            476..1483
                     /locus_tag="AC16V7_03590"
     CDS             476..1483
                     /locus_tag="AC16V7_03590"
                     /inference="COORDINATES: protein motif:HMM:NF028488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR6256235.1"
                     /translation="MKYISRSDLSIILNELIGESRLIAPKKVGGVILYQPITDTSHIT
                     WDFTRPVLSIKESFFPATERLFTIDINGQEVTLNDTLPEIPQIILGVRPCDARGMKIL
                     DALFIDTPPRDSYFAQHRANTTIIGLSCQELGETCFCTSMDGSPTDPQGMDVMLTEIE
                     DGYSVQIITEKGKKFFGDHLSLAYEEAPPPSGTLDRPSAIPAPNDIDWPRNFHEAYWD
                     QLAERCLSCRICAYICPTCRCFTVRDEPIASNDGMQQFERIRCWDACTSEAYRQIAGG
                     HNPRQAKKQRLRNRIYCKFDFYPKQYGPIACTGCGRCVEMCPVNIDVTEVLRDLAEVT
                     P"
     gene            1480..2334
                     /locus_tag="AC16V7_03595"
     CDS             1480..2334
                     /locus_tag="AC16V7_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012402.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HIR6256236.1"
                     /translation="MTTTNTPHNENSMKPSLGRLAYIKDLGTEIKLFGVQLLNGNGAD
                     FHAYHPGQFAFVSAFGIGEAAFGFASIPRNGTLEFAIQRLGSVTTELHELGVGDTVGV
                     RGPLGNCFPLDSMRGKNLIVLGGGIGGAPLRPVIQYVLANRADYGHLTILWAARHPSL
                     LVFTDEYDTWRSAPDTQLHLTVDVADVGWDHHTGLITDLLERVSPSPENTITITCGPP
                     IMIHFVNKMLIEKFGFRPEQNYVTLEARMHCGIGKCGRCNLDEKLVCVDGPVFTMAEV
                     GNFLESFL"
BASE COUNT          676 a          659 c          621 g          684 t
ORIGIN      
        1 attgtactga gcgtctgccg gtcatctatg atacgctcat cggtgaacca ccagcgaacc
       61 aggtggagcc tacaacctcc gcgcgcttat attatttgga aactttatca ccgatagagc
      121 gtatggattt ttggctttcc catttcgatc gctgcatccg ctgctatgcc tgcagacaag
      181 tctgtccgat gtgcgattgc ccaacctgcc tttttgaacg ggacgactcg ctttgggtgg
      241 gcccgggcat caggctagat gaaaaacgaa tttaccacct gggtcgggct taccatcttg
      301 cgggcaaatg cgttgattgt catgagtgcg aacgcgtgtg ccctgtgaat atacctataa
      361 gtttgatcaa tagcaagcta aatctagaga tggagaaatt ctttgggtac cgcgccggag
      421 aacaagctat accatctccc cttcttacaa tccttagcgg agaggagcga ttgccatgaa
      481 atacatctcc cgtagcgatc tctccataat attgaatgag ctaattggcg agtcgagatt
      541 aatcgccccc aaaaaagtgg gcggggtgat cttatatcag ccgataacag ataccagcca
      601 tatcacatgg gacttcaccc gcccagtact atcgatcaag gagagcttct tcccggccac
      661 cgagcgccta tttaccatcg atatcaacgg gcaggaggtc acattaaacg atacgttgcc
      721 cgaaatccct cagattattt tgggcgtcag gccatgcgat gcccggggta tgaagatcct
      781 cgatgcctta ttcatcgata ccccacctcg agattcatac tttgcacagc atcgtgccaa
      841 taccaccatt attggattgt cctgccaaga gttgggcgaa acatgcttct gtaccagcat
      901 ggatggctct ccaaccgatc cgcagggcat ggatgtgatg ctcaccgaga tagaagatgg
      961 ctattctgtc cagatcatca ccgaaaaagg caagaagttt tttggtgacc atttatcact
     1021 ggcctatgaa gaggcgcctc ccccctcagg cactcttgat cgcccatctg ccatccctgc
     1081 acccaacgat atcgattggc ctcgtaattt tcacgaggcg tactgggatc agttagcaga
     1141 gcgctgcctg agttgtcgta tctgcgcata catttgccca acatgccgtt gttttaccgt
     1201 gcgcgacgag cccatcgcca gcaatgatgg catgcagcag ttcgaacgaa ttcgctgttg
     1261 ggacgcctgc actagcgaag cctatcgaca aatagccgga ggtcacaatc ctcgccaggc
     1321 aaagaaacag agattgcgca accgcattta ttgtaaattt gacttctacc caaagcaata
     1381 cggtccgata gcctgtaccg gatgtgggcg ttgtgtggaa atgtgcccgg taaacatcga
     1441 tgtcaccgaa gtccttcggg atttagcaga ggtgacgcca tgaccacaac aaatactccc
     1501 cataacgaaa attctatgaa gcccagtttg ggacggcttg cttatatcaa agatttaggc
     1561 actgagatca agttgtttgg agttcaatta ctgaatggca atggtgctga tttccatgct
     1621 taccatccag ggcaatttgc ttttgtatcg gcatttggta taggggaggc tgcctttgga
     1681 tttgcttcaa ttcccaggaa tggaaccctt gagtttgcca tccagcgcct gggctcggtg
     1741 accaccgaat tgcacgaact cggtgtaggt gataccgttg gggtgcgggg tccgcttggc
     1801 aattgctttc cgcttgatag tatgcgaggt aaaaatctta tcgttttagg tggtggtatt
     1861 ggaggagcac ctctacgccc cgtaattcaa tatgtgctcg caaaccgcgc tgattatggt
     1921 catcttacca tcctctgggc agcccgtcac ccctcgttgc tggtatttac tgacgaatac
     1981 gatacctggc ggagtgcgcc agatactcag ctgcacctga ctgtggatgt ggctgatgta
     2041 gggtgggacc accatacagg cttaatcaca gaccttttag aacgagtttc accttctcct
     2101 gaaaacacaa ttacgatcac atgcggacca ccgatcatga ttcatttcgt caacaaaatg
     2161 ctaatcgaaa agtttggctt ccgcccggaa caaaactacg ttaccctgga ggctcgcatg
     2221 cattgcggca ttggcaagtg tgggcgatgc aatctagacg agaaactagt ttgtgtggat
     2281 ggaccggtat ttacaatggc tgaagtaggt aattttctgg agagcttctt gtaaggataa
     2341 ggaacaacct gtcctctatc tcaagacaaa tggagtatcc cgtccagttt tccctttcca
     2401 tccacctagg ttacttccga tcaatgttga tttcaccaaa aatcttggct aaatcaacat
     2461 ataaaactcg atacattcac gaaaaaaccc tcttctagag aaaccgaaga gctacacata
     2521 cccaacttga cgattgtact cagttaccat ggacaatgtg ttgacaatcg attggaattg
     2581 ctatacactc tttcttgtag gatagtgcga taaaacacaa taatttgcag ttaaatactc
//
LOCUS       DBNHOH010000200         2539 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1613803, whole genome shotgun sequence.
ACCESSION   DBNHOH010000200 DBNHOH010000000
VERSION     DBNHOH010000200.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1613803"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2512
                     /locus_tag="AC16V7_03600"
     CDS             <1..2512
                     /locus_tag="AC16V7_03600"
                     /inference="COORDINATES: protein motif:HMM:NF017539.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR6256237.1"
                     /translation="SFELDRLSFWLGFIAGILLIWLLGRLRPWVARLFQSLRERSQLS
                     KLGAATVTEIRLRNDLLRYTQGLHLASPLFSLDEISLTPRLLAPPALTEPGITPPSID
                     IAENILPPLPDAPELASFYGAPSFTALEALGGNAHLLIIGHSGSGKTVTLAKLCADII
                     QADQLPSNLKDSLPILLHAADITLPSSNPDDLLEPLVIAVAQICPSVPQARLEKFLPA
                     AFSGGRTLLLLDGLDELPPADVTIYVEYIKNLLDHYPDTRLIATATPDFFDGLTAIGC
                     VPLTLASWNDKQRREFINRWSDMWINHIGSMTKDTEALDPNLLNAWLTNDNSILSPLE
                     LTLKVWATYAGDLPGPHKTDWIEAYLQRVTVEIPKGRPALELLTRHAVNTRKPIFTLR
                     DAQNWLAEIEPSPTPDAAPGVEVKDEDANWQPIDTERPKSSPQAPIRDSRVLPALIES
                     GLLISHQNETLSFSHPLILSYLAACGFAKNGGVSSMTKDSQWPVYLQTLGFLATKSPD
                     ADSFVRPYFEKSPEPLMRDIFTAARWLRYAPESASWRAHIMRQLARVMQNESYPMALR
                     ARALTALTLSGSSGVDTLFRQFLTSKDNALRQLGALGCGALQDSKSINDLISLLGQNH
                     PNLRQAACLGLVAIGNQTALEAVAESLLNGDDELRQTAAEALANNIEEGHPTLKEGAT
                     LEDFLVRRAVVYGLRRVGQPWAIQTLERMQVSDEQWVVKDAAARVLDELAKPKVRLPK
                     SMPDLTETPWLIAFAGERGIGISPGKPALDLLLLALKEGNEEQRLSALDYLARYGEAN
                     AIPVILEIYATQRGEVCEAAYNTLWHIAASGVELPTLS"
BASE COUNT          626 a          686 c          593 g          634 t
ORIGIN      
        1 cagctttgag ttagaccggc tctccttctg gctgggtttc attgccggaa ttctcctcat
       61 ttggctactt ggcagactcc gtccatgggt ggcgcgcctg ttccaatcct tgcgcgagcg
      121 ctcacaacta tccaaattgg gagcagcaac ggtcaccgag atccgcttgc gtaatgatct
      181 cctgcgttac actcaaggtt tacatctggc ctcgccgcta ttctctctgg atgagatctc
      241 ccttaccccc cgcctgcttg caccgcccgc cctgactgaa cctggcatca ctccaccttc
      301 aatagatatt gctgagaata ttcttcctcc cctaccagat gccccagaat tggcttcttt
      361 ttacggcgcg cccagcttta ctgctctcga agcgctggga ggaaacgccc acctgctgat
      421 tatcggccac tcggggagcg gaaaaactgt caccctggct aaactctgtg cggacatcat
      481 tcaagcagac cagctcccgt ccaatcttaa ggatagtctg ccaatactgc ttcatgccgc
      541 tgatattacg ctcccatcct ctaatcccga cgatttatta gagcctttag ttattgccgt
      601 cgcccagatt tgtccatctg tcccccaggc acgcctggaa aagtttcttc ctgccgcctt
      661 ttctgggggc agaacgctac tcttgttgga tggattagac gaattgcccc ccgccgatgt
      721 aaccatatat gtggaatata ttaagaattt gctcgaccac taccctgata cacgccttat
      781 cgccactgca acaccagact tcttcgatgg tttaactgcc atcgggtgcg tgccccttac
      841 tctggcaagc tggaacgata aacagcgccg agagtttatc aatcggtgga gcgatatgtg
      901 gatcaaccac atcggatcaa tgactaagga tacagaagcc ctcgatccaa acctgctcaa
      961 tgcctggcta accaacgata attccatcct ttctccactc gaattaactc tcaaagtttg
     1021 ggcaacatac gctggcgatc tacctggtcc acataaaact gattggatcg aagcctattt
     1081 gcaacgtgtg acagttgaaa taccaaaagg tcgtcctgct ttggagctct taacccgcca
     1141 tgcggtcaac acaagaaaac ctatatttac gctgcgggac gctcagaatt ggctagcaga
     1201 gatcgagcca tctccaactc ctgatgcagc gcccggagtt gaagttaaag atgaagatgc
     1261 caattggcaa cctattgata cggaaagacc aaaatcatcc cctcaagctc caatccggga
     1321 ttcgcgcgtt cttccagcct taattgagag tggtttgcta atttcccacc aaaatgaaac
     1381 tttgtccttt tcccatccct tgatcctcag ttacttggcg gcctgtggct ttgcgaaaaa
     1441 cgggggggtg agcagcatga cgaaagatag ccagtggcct gtctatttgc aaacacttgg
     1501 atttttagct acaaaaagcc ctgatgcaga ctcttttgtg cgaccttatt ttgaaaagag
     1561 ccctgagcca ctgatgcgcg atatattcac cgcagcaaga tggctccgtt atgctcccga
     1621 gagtgcgtct tggcgtgccc atatcatgcg gcaattggct agagtaatgc aaaatgaaag
     1681 ttacccaatg gctctacgcg ctcgcgcact cactgcgctt accctctcag gcagctctgg
     1741 agtagataca ctatttcgcc agtttctcac ctctaaggat aatgcgttgc gccaattagg
     1801 cgctttgggt tgcggtgctc tgcaagattc caagtccatc aacgacttaa ttagcctgtt
     1861 gggacaaaac caccccaatt tgcgccaagc cgcatgcttg gggctagtcg caattggtaa
     1921 ccagacagcg ctggaggcag tagctgaatc cttgttaaac ggcgatgatg aacttcgcca
     1981 aacagccgcc gaggctttag ccaataatat tgaggaaggt cacccaacgc tcaaagaagg
     2041 cgcaaccctt gaagattttc tggtacgacg cgcagtggta tacggattgc gacgggtagg
     2101 ccagccttgg gcaatccaaa cattggagag aatgcaagta agcgatgaac aatgggtggt
     2161 taaggatgca gccgcacgcg tattggatga gttagccaaa ccaaaggtac gcctaccaaa
     2221 aagcatgcct gacttaacag aaacgccttg gctgattgca tttgctggcg agcgtggtat
     2281 tggtatctca ccaggtaaac ctgccttaga tttgctcctg ctggcactga aagaaggaaa
     2341 tgaggaacaa cgcttatccg ccctcgatta cttggcacgt tacggagagg caaacgccat
     2401 tccggttatt ttggaaatct atgcgaccca gagaggggaa gtatgcgaag ccgcctataa
     2461 cacgctttgg cacattgcgg cttcaggggt tgagctacca actctatcgt gattttgtta
     2521 ttagttactt aagtgagta
//
LOCUS       DBNHOH010000201         5370 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_203860, whole genome shotgun sequence.
ACCESSION   DBNHOH010000201 DBNHOH010000000
VERSION     DBNHOH010000201.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5370)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_203860"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="AC16V7_03605"
     CDS             <1..962
                     /locus_tag="AC16V7_03605"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6256238.1"
                     /translation="PYYHVLGFNVMLYAMMATSWNIMGGYTGYKSLGHSAFYGLGAYL
                     VAIAANQLGWNPLLSAPLLAFVVALVAILLGWIMLRTSGSAFVIATIAMLLIFRLTAL
                     NLREITKGAPGLSQPLPPWTPEFSRMPFYYYMLLWLIISIFVSWYISRSRFGLGLVAI
                     RDDEGKAEMVGVNTTLYKILAFGVSAYFVALGGGVWSYFTSHISPVFAFDIIVGVDMI
                     LMTMLGGLGTLWGPVLGAFILIPSSDLILFSFGSNTIYLAIIGALMITVIIFLPKGII
                     PTIQQRIQDHRAPRAAHEGAISMAEMHGLTDESASLVEENPHD"
     gene            955..1689
                     /locus_tag="AC16V7_03610"
     CDS             955..1689
                     /locus_tag="AC16V7_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012596637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6256239.1"
                     /translation="MTELLYTNGISRRFGGIQAVKDCNISVQAGSITGLIGPNGSGKT
                     TLFNLITGVYAPDEGEAYFDGKRITNLSPSKICHAGLSRTFQITRLFWEMTVLENMIV
                     PIRQVGLGSMLGPNMFKEEIDRAIGLLEHVGLTHLKDEPARKLSFGQQKLLEFAAILM
                     ANPTMVLLDEPAGGVNPTMIRFIMKLIQELNQDGVTFLVVEHNMGVVMELCNHVVVMD
                     QGTKLAEGAPEAIQANPAVLDAYLGN"
     gene            1692..2417
                     /locus_tag="AC16V7_03615"
     CDS             1692..2417
                     /locus_tag="AC16V7_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6256240.1"
                     /translation="MLLEIKDVVAGYLPDVNVLQGINIHVEPSELVCMIGPNGAGKST
                     VLRTISGLLQPKGGSIAFNGKEIGGLRPDLILHQGIAHVPQGHSSFPAMTVQENLLMG
                     AYILRNRAERNRRMGKVFEMFPILKERKNEKAGNFSGGQQKILEMGRALMLEPKMILL
                     DEPSLGLAPKTAKQVFDTIKSLNDEMGMTVLLVEQNAKSGLAIADRAYVLELGIERLE
                     GPASTLLNDPRVAELYLGGTLKS"
     gene            2422..3327
                     /locus_tag="AC16V7_03620"
     CDS             2422..3327
                     /locus_tag="AC16V7_03620"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR6256241.1"
                     /translation="MSGALWAITAGVGFGLFQTFNRRAVRGMDVYVATFIQLLVSTIV
                     LLAIVLLTEDIGALLVAPFSVWVNFALAGILHFFLGWTFLNASQKRVGAARTSPLIGT
                     TPLFATAIAAVTLREFPGIWGIIGIGLIVLGAYVISQRSGDSREIEGNPQPIKAGWQA
                     SWLGLSAALCWALSPIFIRAGLEDLPSPLLGVTWGIAFSALAYGIVLLWQRDRWLGSE
                     ITGDAWMFKLLAGLLVGLSTWVRWIALDLAPVAIVIALTMISVPLVILLSPLVSGKQM
                     ESVTVSLWVGAGLILGGALLLTFIV"
     gene            3351..4334
                     /locus_tag="AC16V7_03625"
     CDS             3351..4334
                     /locus_tag="AC16V7_03625"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6256242.1"
                     /translation="MIIDTHAHIIVPEILQDAKPNEAWRPRVVWENGKQFVEYKSKRI
                     GSATREFVSVETILSEMDKSGVDAVLLCPWVSLVRYEAAPEESLDACQVQNDAMAELA
                     AKYPKKIAGLGMLPLQDVPLAVRELERLMKSGLKGVEIGTHVNGIYPGDERFRPFWEA
                     CEALGVFVFIHPVEGGGRAELRDYYMWNVIGNPLETTVAAGHLILSGVMDAYPRLNIL
                     LAHGGGALPYIHGRLDRGFKQRPEINKFISRPPTEYLKLFYFDTITHDPEVLRNLVDL
                     VGADHVLLGSDYPFDMGNEKPADLVRAAGLGADAEAKILGATAMQMLHWEV"
     gene            4335..4709
                     /locus_tag="AC16V7_03630"
     CDS             4335..4709
                     /locus_tag="AC16V7_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012417.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6256243.1"
                     /translation="MARLFTFEQLPHLHSTRDTRDRLDLVTDNVPVNAQAIRADRIIY
                     HPGDTAAKHYHTDCHHLFFVLYGNGILHVDGGSYRLAPGKVAVVAPNEIHWFENDTDE
                     NFAFVEFWGPPPKETVWITDDV"
     gene            4776..>5370
                     /locus_tag="AC16V7_03635"
     CDS             4776..>5370
                     /locus_tag="AC16V7_03635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6256244.1"
                     /translation="MDMHMMDLSDEQRQMRAICREFVDNEVIPFIRANRENEWTTPPE
                     ERQPWELLKAADKLGLRALGVPEKYGGIQLEKQAQTFAIIAEELSRGDSGFADIISQL
                     WKVPVLLSNVAPEHLQDEWFPRFMDDPTFLMAHCLTEPRGASDRWLPYNVPEAGMHTK
                     AVLQDGYWVINGRKQFITNGYDAKLFVVYANTNPAVGI"
BASE COUNT         1211 a         1353 c         1471 g         1335 t
ORIGIN      
        1 cgccctacta tcatgttttg ggattcaatg tcatgttgta tgcgatgatg gcaaccagtt
       61 ggaacattat gggcgggtat acgggttaca aatccctcgg ccatagcgcc ttctatgggc
      121 tgggtgccta tctagtggcc atcgcagcca atcaactggg ttggaaccca ttgcttagtg
      181 cgcctttgct ggcgttcgtc gtcgccctgg tcgctatctt gctggggtgg atcatgctgc
      241 gcacaagcgg ttctgctttt gtcattgcga cgattgctat gctgctcatc ttccgcctaa
      301 cagccctgaa tctccgagag attaccaaag gcgcaccagg tttgagccaa ccactcccac
      361 cctggacgcc tgaattttct cgcatgccgt tctactatta catgctgtta tggctgatta
      421 tctccatctt tgtctcctgg tacatcagcc gctcccgctt tgggctggga ttggttgcca
      481 ttcgagatga cgaaggtaaa gcagagatgg tgggcgtcaa cacaaccttg tataagatcc
      541 ttgccttcgg tgtcagtgcc tattttgtgg cgctgggcgg tggcgtatgg tcctatttca
      601 cctcccatat cagcccggtt tttgcgttcg atattatcgt cggggttgac atgatcttga
      661 tgacgatgct gggtggtctg ggcacgcttt ggggacctgt gctcggcgca ttcatcctca
      721 taccatcctc ggatctgatc ctattcagct tcggttcaaa caccatctac ctggcaatta
      781 ttggcgcttt gatgataacg gtgatcatct ttttgccgaa gggcatcata ccaaccatac
      841 aacagcggat acaagatcac cgtgccccac gcgcagcgca tgagggagca atttcaatgg
      901 ccgaaatgca tggcttaaca gacgagtccg cctccctcgt cgaggagaat ccccatgact
      961 gaattactct acacaaatgg gatttctcgt cgtttcggcg gtattcaggc tgtcaaggat
     1021 tgtaatatct cggttcaagc gggcagcatc accgggttga tcggtccaaa cggctcggga
     1081 aagaccacct tatttaactt gatcacaggc gtctacgccc ctgatgaagg ggaagcctat
     1141 ttcgacggga agcgcattac gaacttatct cccagcaaaa tttgccacgc aggactcagc
     1201 cgaaccttcc agatcaccag gcttttctgg gaaatgacag tgctcgaaaa catgatcgtc
     1261 cccatccgcc aggttggttt aggttcgatg cttggcccca acatgttcaa agaagagatc
     1321 gatcgcgcaa ttgggctcct ggaacacgtc gggctcacgc atctgaaaga tgagcctgcc
     1381 cgcaagctgt cttttggtca gcagaagctt ttggaatttg ccgcgatctt gatggcaaat
     1441 cccaccatgg ttttgctaga tgaaccggcg gggggagtaa accccacgat gatccgtttc
     1501 atcatgaaat tgatccagga actcaaccag gatggcgtga cttttctggt tgttgagcac
     1561 aacatgggcg tagtcatgga actttgtaat catgtcgtcg tgatggatca aggcacaaaa
     1621 cttgctgaag gcgcgccaga agcgatccaa gccaacccag ccgttttaga cgcataccta
     1681 ggaaattaac aatgttactc gagataaaag atgtcgtcgc cggatacttg ccggacgtga
     1741 atgtcctcca aggcatcaat atccatgtag agccaagcga actggtatgc atgatcggtc
     1801 caaatggcgc aggcaaatcc acagtactgc gtacgatctc tggcctgttg caacccaaag
     1861 gcggaagcat tgcttttaat ggaaaagaga ttggaggtct acgtcctgat ttgatcttgc
     1921 atcaggggat tgcccatgtc ccgcaggggc acagctcatt cccagcgatg accgttcagg
     1981 aaaatctgtt gatgggggct tatatattgc ggaacagggc tgaacgtaac cgccgtatgg
     2041 gcaaagtgtt tgaaatgttc cccattctga aggagcggaa gaacgaaaaa gccgggaatt
     2101 ttagcggcgg acagcagaag atcctcgaaa tgggacgggc actgatgctc gaaccgaaga
     2161 tgatcttact tgatgaacct tcgttgggac tggccccgaa aaccgccaaa caggtttttg
     2221 ataccatcaa atccctgaac gatgaaatgg gcatgacagt gttgttagtt gagcagaatg
     2281 ccaaaagcgg attggcgatt gctgatcgcg cttacgtgct ggaactgggg atcgaacgtt
     2341 tggaaggccc tgcttccacc ttgctcaacg accctcgcgt ggccgagctt tatctgggtg
     2401 gcacactaaa gagctaaacc tatgagtgga gctttgtggg ccatcaccgc cggcgtgggt
     2461 tttgggcttt tccagacttt taaccgtcgc gcggtgcgtg ggatggacgt atatgtcgcc
     2521 acgttcatcc agttgctggt ttcaacgatc gtcctcctag caattgtcct actgacagag
     2581 gatataggcg ccctgcttgt tgctccattt agcgtctggg tgaattttgc tctagccggt
     2641 atattgcatt ttttcttagg gtggactttt ctgaacgcca gccagaaaag agttggagcc
     2701 gcgcgcacaa gtcccttgat cgggacaacc ccgctctttg caaccgccat cgctgcagtg
     2761 acgctgcgcg aatttcccgg tatttggggc attatcggca ttggcctgat cgtactaggg
     2821 gcttacgtga tttctcagag atcaggcgat tcgagagaaa tcgaaggcaa tccacaaccg
     2881 atcaaggctg gctggcaggc ttcctggctg gggcttagcg ctgctttatg ttgggcactc
     2941 agcccaattt ttatccgagc tggcttggaa gacctgccct ctcccttgtt aggtgtcaca
     3001 tggggcatcg cattcagcgc gctagcgtat gggattgttc tgttgtggca gcgagatcgc
     3061 tggctcggat cggagattac tggcgatgcc tggatgttta agcttctggc aggtttgctg
     3121 gttggcttat ccacctgggt gcgttggatc gcgctcgatc tagctccggt cgcaattgtg
     3181 atcgctctga ccatgatatc ggtgccgctg gtgatcttgc tctccccact cgtgtctggc
     3241 aagcaaatgg aaagtgtgac cgtctccctg tgggtaggag caggtctgat tttgggtggc
     3301 gcgttgctgt tgacttttat cgtctgaatc gagaaggagt ttctagatac atgatcattg
     3361 atactcacgc tcacattatt gtccccgaga ttcttcaaga tgcaaaacct aacgaagcgt
     3421 ggcgtccgcg cgtggtgtgg gagaacggca aacaatttgt agagtacaaa agtaagcgaa
     3481 ttggttctgc gacacgcgag ttcgtatcgg tggaaaccat actgtccgaa atggacaaat
     3541 ctggcgtgga tgcggtcttg ctctgcccgt gggtctcact ggtgcgctac gaagctgcgc
     3601 cagaagagtc gctggatgca tgccaggtac agaatgatgc tatggctgaa ctggcggcaa
     3661 aatatcccaa aaagatcgcc ggccttggca tgttgcctct ccaagatgtc ccactggctg
     3721 tgcgcgaact ggaacgtttg atgaagtcgg ggctgaaggg tgtcgagata ggcacgcatg
     3781 tcaacggtat ctatcctgga gacgagcgtt tccgtccatt ttgggaagcc tgcgaagcgc
     3841 ttggtgtgtt cgtatttatc catccagtgg aaggtggggg acgcgccgaa ttacgcgact
     3901 actacatgtg gaatgtgatc ggcaacccgc tggaaaccac cgtcgctgcg ggacatctaa
     3961 tactgtctgg cgtgatggac gcatatccgc gtctgaatat tttgcttgca cacgggggcg
     4021 gggcactgcc ttatatccac gggcggctgg accgcggctt caagcagcgc ccggagatca
     4081 acaaattcat ctcccgccca ccgactgaat atctcaagct attctatttc gataccatca
     4141 ctcacgatcc cgaagtgctg cgcaacctag tggatctggt gggcgccgat catgtcctgc
     4201 tcggttcaga ttatcctttt gatatgggga atgagaagcc ggcagatttg gtacgcgcgg
     4261 ctggtcttgg ggcagatgct gaagcgaaga tcctgggtgc aaccgcgatg caaatgctgc
     4321 attgggaggt ttagatggct cgtctcttca cgttcgagca attaccacat ctgcactcca
     4381 ctcgcgatac tcgcgaccgg ctggaccttg tgacagataa tgtcccagtc aatgcgcagg
     4441 caatccgcgc tgaccgcatc atttatcatc ccggcgatac ggcagccaaa cattaccaca
     4501 ctgactgcca tcacctcttc tttgtgctgt acggtaatgg catcctgcac gtggacgggg
     4561 gaagttatcg cctcgcccca ggcaaggtgg ctgtcgtcgc ccctaatgaa attcactggt
     4621 ttgagaatga cacggatgaa aattttgcct tcgtcgagtt ctggggacca ccgccgaaag
     4681 agacggtttg gataacagat gatgtctgaa cttgggagtc acacgcctga gttgcaggcg
     4741 ttctatataa cgcgaaaaac acaggaggta cgcggatgga catgcacatg atggatctgt
     4801 ctgatgaaca gcggcaaatg cgtgcaatat gccgtgagtt cgttgacaac gaggtaattc
     4861 cttttatccg tgccaatcga gagaatgagt ggacaacccc accagaggaa cgccaaccct
     4921 gggagctgct gaaagcagct gataagctcg gcctgcgcgc cctaggtgtg ccggagaaat
     4981 acggtgggat acaactggaa aagcaggcac agacattcgc aatcattgcg gaagaactgt
     5041 cgcgcggaga ttcgggcttt gccgatatta tcagccagct atggaaagtg cccgtcctgc
     5101 ttagtaacgt agcgccggaa catctccagg acgagtggtt tccgcgcttc atggacgatc
     5161 ccacattctt gatggctcac tgcctcactg agccgcgcgg tgcttctgat cgctggctcc
     5221 cctataacgt gcctgaagcc ggcatgcaca ccaaagctgt tctacaggat ggatactggg
     5281 taattaatgg gcgcaaacaa tttatcacca atggttacga tgcgaaactg tttgtggtct
     5341 atgccaatac taaccctgcc gtcgggattg
//
LOCUS       DBNHOH010000202         2155 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1165507, whole genome shotgun sequence.
ACCESSION   DBNHOH010000202 DBNHOH010000000
VERSION     DBNHOH010000202.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1165507"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(259..1212)
                     /locus_tag="AC16V7_03640"
     CDS             complement(259..1212)
                     /locus_tag="AC16V7_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256245.1"
                     /translation="MWLSGLVTYAQAEQILREIGRVDISQSSVWRRVDLWGRRLQAIE
                     SLQQAKAYGFEEPQEIDPNRSLGRMGAAMDGTMVHIREEGWKELKVGCVFDIAKRRQY
                     DPHTQEVIELGSAIHNGYVAHLGGPEVFGGKIWAEARQRHWMHAEDSLTMGDGALWIW
                     NLVSEHFYASHQLVDWYHATEHLANAAKTLNGEGTPAAQRWYRHWETKLYQGHVDKLV
                     QALQSQAERQPDKAEVLRKEAGYFHNNRKRMNYLEMRAEGYPIGSGMVESAAKQYKAR
                     FTGPGMRWSRTGIDRLLPVRTAIMSGRFNKMWRLAYNSPPN"
     gene            complement(1232..1615)
                     /locus_tag="AC16V7_03645"
     CDS             complement(1232..1615)
                     /locus_tag="AC16V7_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256246.1"
                     /translation="MKKSQGRLKAEFMAEAEALFDQLMEWDEQTAKPKLTQIEEIVLQ
                     LRQRLGEQMVQAVLARQENRQPAEKLSCPQCQGELEPKGEKGKRVESQVGSLQIERSY
                     YYCPGCRQGFFPSGSATTDLGETLE"
BASE COUNT          491 a          617 c          472 g          575 t
ORIGIN      
        1 ccaaaaagag taatctgcaa ggtgctcatt ctcctcctcc ataaaactat ccaattcgaa
       61 ggtattttgc agcaagtctg agcacctcga aaatatcatg ccatattata tatttatccc
      121 ctcatgagca ttgagatgcc aagccaagag gattttattc agaataggga aaattcaata
      181 ttaaatacag acaatcattg tctgtattat agtgaattta tgatatttgt caacaaggta
      241 atctgacctg ggcgcatttc aattcggggg tgaattgtaa gccaggcgcc acattttatt
      301 gaaacgacca ctcatgatcg ccgttcggac aggcagaagg cgatcaatac cggtgcggct
      361 ccaacgcatc cccgggccag tgaaacgagc cttgtattgt ttggcagcgc tctccaccat
      421 gccactgccg atcggatatc cttcagcgcg catttccaga taattcatgc gtttgcggtt
      481 gttgtgaaag taaccggctt ccttgcggag cacttctgcc ttgtcaggct gccgttcagc
      541 ctgactttgt agagcctgca ccagtttatc tacgtggccc tggtagagtt tggtttccca
      601 gtggcgatac cagcgctggg cagccggcgt gccttcgccg ttcaaggtct tggctgcgtt
      661 cgccagatgc tcggtagcat gataccaatc caccaactgg tggctggcat agaagtgttc
      721 acttaccagg ttccagatcc acaaagcacc atctcccatg gtcaggctgt cttcagcatg
      781 catccaatgt cgctgacggg cttcagccca tatcttgcca ccaaacactt ccggtccccc
      841 taaatgagct acgtaaccgt tgtggattgc acttcccaac tcgatcactt cctgggtgtg
      901 agggtcatat tggcgacgtt tagcgatatc aaacacacaa ccaaccttca attccttcca
      961 gccttcttcc cggatatgca ccatcgttcc atccatggct gcccccatgc gccccagcga
     1021 gcggttggga tcgatttctt gcggctcttc gaacccataa gccttagcct gttgtaagct
     1081 ttcaatggct tgcagcctgc gcccccaaag atcaacccgc cgccagacgc tgctttgcga
     1141 tatgtccact cgcccgattt cccgcaagat ctgttcagcc tgggcatagg tcaccaatcc
     1201 actcagccac accgcctgtt tagcaacctg ctcactccag tgtttctccc agatctgtag
     1261 ttgctgatcc agaggggaaa aaaccctgcc gacaaccagg acagtagtaa tagcttcgct
     1321 cgatctgcag gctgcctacc tggctttcaa cccgcttccc cttttccccc ttgggttcta
     1381 actcaccctg gcattgtgga cagctcagtt tctccgctgg ctgacgattc tcttgccggg
     1441 ctaacaccgc ttggaccatt tgctctccta accgttggcg taattgcaac acgatctctt
     1501 cgatttgggt taatttcggc ttggcggttt gttcatccca ctccatcaat tgatcaaaaa
     1561 gcgcctcagc ttctgccata aactcagctt tcagccttcc ctgacttttc ttcatcgctc
     1621 acctctctac cactaatttc cttattgtgc catagttttc ccccaaaact gaattacgcc
     1681 cctaaccctc gcgcgagggt ttttacgagg tagaaaatcc tgcgcaagca ggctcaaccc
     1741 cattcccaaa tcccaccccc aagcgattgc gcgatgctcc agggcctggg gaaggtgctt
     1801 gatgaatttt atggggttat gtaggcgggc aagccgcccg cattgcccca gaccccaaaa
     1861 aagtgcaagc cactttgtga aagccgtgtt cctgcccgct agtcacaggg atatatatgt
     1921 gagtgacgcg gcccactagt gagcatgggg gtgcaaaatt gaattgactt ttccaatctt
     1981 ctcggatacc ccccttagaa ttaccagtgc acatagggga gcaggaagca tggtagcaat
     2041 caccgaccgc ctcaaaagtt cagcaaattt ttatgcattt gcgtttttcg cgtaaatatt
     2101 aatgcgtcta aatgtttcga agtatccagc attctcttgg gctgtgtttt ttagc
//
LOCUS       DBNHOH010000203         2550 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_524726, whole genome shotgun sequence.
ACCESSION   DBNHOH010000203 DBNHOH010000000
VERSION     DBNHOH010000203.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_524726"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="AC16V7_03650"
     CDS             <1..208
                     /locus_tag="AC16V7_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256247.1"
                     /translation="RSGDRMACITAKGNAMLVIRGAFIAAIMSGYRRVEILVKRGKIF
                     RVRRGHVERIPHKQGINSIGLSRL"
     gene            351..2048
                     /locus_tag="AC16V7_03655"
     CDS             351..2048
                     /locus_tag="AC16V7_03655"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC1 kinase family protein"
                     /protein_id="HIR6256248.1"
                     /translation="MIRQRYRLITGFFARLLLSFAYWDLILPRLGMRKWAKKTRPERL
                     RQSARAFRGLAIQLGGVMIKVGQFLSARVDVLPKEITDELEGLQDEVPPEKFEDIRRV
                     VEGEFGAPLSEKYAYFDEQPLAAASLGQVHSARLNLKSENSDEKDPPKQAEGIFDVVV
                     KVQRPNIENIIATDLAALRTVGNWLKRYRPIRLRADIPALLDEFTRVLYEEIDYLAEG
                     RNAETFAANFKSNVWVKVPTVMWTHTTRRTLTLENVLAIKITDYVAITSAGIDRSEVA
                     SRLLDVYLRQIFEDGFFHADPHPGNLFVSPRDEKDLESEVPWQLTFVDFGMVGRVPPQ
                     TREGLRELIIGVGTQDANRVVKAYQMLGVLLPGADLELLKKMEAKAFEQFWGKTMSEL
                     QQISTHQVRKFASEFRELLYSMPFQVPQDFVFLARCVGILSGMCTGLDPQFNVWTHLA
                     PYAQKLIAEEARVGWDFWWDEIKKQVKVAFSVPLKMDAILSQLERGDIAVRTPEVIRR
                     ITALDWSIRKLIGAILFAALLLSGTQFYLVGKSPLGEILLLGAGLCLLWVLFIQRRV"
     gene            2148..>2550
                     /locus_tag="AC16V7_03660"
     CDS             2148..>2550
                     /locus_tag="AC16V7_03660"
                     /inference="COORDINATES: protein motif:HMM:NF013247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR6256249.1"
                     /translation="MKSDFVWMDGDLVPFEEAKVHVINPTLHYGPGVFEGIRCYETPK
                     GPAVFRLRDHLERFIESIHILGVLKFPYTIDELQRGVHTTIRVNGFSECYVRPLMYLD
                     GPLGLNMDRSKARVFIAAWEWGPYLGEEAQEK"
BASE COUNT          628 a          529 c          699 g          694 t
ORIGIN      
        1 acgcagtggg gatcgtatgg cgtgtatcac agcaaaaggg aacgccatgc tcgttatacg
       61 gggcgcattt attgccgcaa tcatgtctgg ttatcggaga gttgaaattc tggtaaaaag
      121 aggcaaaatc ttcagggtta ggagggggca tgttgagagg ataccacaca aacaggggat
      181 aaattccatt ggattgagta gattataacc ttcatatatg tcctttttgg tttcaatgat
      241 ccttgggttg tttgggcttc atagggtcat atatcgtttg tttcgcttgt tccacgtaat
      301 acctcatagg cttaaagttg atcatctaaa agggtataat ccctgatgct atgatccgcc
      361 agcggtaccg actcattacg gggttttttg cgcgtctgct gcttagtttt gcttactggg
      421 atctcattct cccccgcttg gggatgagaa aatgggcaaa gaaaacacgc cctgagcgct
      481 taaggcaaag cgccagggcg tttcggggtt tagcgatcca attgggcggg gtgatgatca
      541 aagtgggaca atttctctct gccagggttg atgttctacc gaaagagatc accgatgagc
      601 tagaaggctt gcaggacgag gtacccccag agaaatttga ggatattcgc cgagtggtgg
      661 aaggggaatt tggcgcccct ctgtccgaga aatatgcgta ctttgacgag caaccattag
      721 ctgctgcttc tctgggtcag gtgcatagtg caaggctcaa cctgaaatcc gagaattctg
      781 atgaaaaaga ccctcccaag caggcagaag gcatttttga cgtggtggtg aaagttcaac
      841 gtccaaacat tgagaatatt atcgccaccg acctggccgc cttgcgtact gtagggaact
      901 ggcttaaacg ctatcgacct atccgcctac gggcggatat tcctgccctg ttagatgagt
      961 ttacgcgggt cctctacgag gagatcgatt acctggctga gggtcgtaat gctgagacat
     1021 ttgcggcgaa cttcaagtcc aatgtatggg ttaaagtccc aaccgtgatg tggacgcaca
     1081 ccacgcgccg cacattgacg ctggaaaatg tgttggctat caagattaca gattatgttg
     1141 ccattacctc cgcaggcatt gatcgatccg aagtggcttc tcgattgctg gatgtctacc
     1201 tgcgccagat tttcgaggat ggtttctttc acgccgatcc acacccaggt aatttgttcg
     1261 tctcgccaag ggatgaaaaa gaccttgagt cggaagtacc ctggcagctc acgtttgttg
     1321 attttggtat ggttgggcgc gtcccacctc aaacccgaga agggttgcgt gaattgatta
     1381 tcggcgtggg cacacaggat gcgaatcggg tggttaaggc ataccagatg ttaggtgtcc
     1441 ttctcccagg agcggacttg gaattgttga agaaaatgga ggccaaggct ttcgaacaat
     1501 tctggggaaa aacgatgagt gagcttcagc aaatcagcac acaccaagtg cgcaaatttg
     1561 ccagtgaatt tcgcgagttg ctctactcca tgccttttca agtgcctcaa gattttgtat
     1621 tcctagctcg ttgcgtgggg atactttcgg gaatgtgtac tggtcttgac ccgcaattca
     1681 acgtgtggac acatctggca ccgtatgcac aaaagttaat agctgaggag gccagagttg
     1741 gctgggattt ttggtgggac gagatcaaaa agcaggtgaa agtggcgttt tcagttccat
     1801 tgaagatgga tgcgatatta tcccaattag agcgggggga tatcgctgtg cgcactccgg
     1861 aggtcatccg caggataaca gcgctggatt ggtctatccg caagcttatt ggtgctattc
     1921 tgtttgctgc gttgcttttg agtggaaccc agttttatct ggttggaaag agccctttag
     1981 gggaaatttt attgctaggc gcaggtttgt gtctcctatg ggtgctattc atacaaagaa
     2041 gagtttaaag tatattatta ttgcgatacc tggatttgaa ataaccaccg aacaaataat
     2101 ctgatccgtc tattggggtt ctacatgcga aaataaagga gttctaaatg aaatccgatt
     2161 ttgtttggat ggatggagac cttgtgcctt ttgaggaggc gaaggttcac gtgatcaatc
     2221 caactttgca ctacggccca ggtgtatttg aaggaatccg ctgttatgag acgccgaaag
     2281 ggcctgctgt gtttcgttta cgagaccacc tggagcgttt tattgaatca attcatatac
     2341 ttggagtgct caaattccca tatacaatcg atgaattgca acgcggtgtt cacactacca
     2401 tccgagtaaa tggctttagc gagtgttacg tgcgcccgtt gatgtatctg gatggacctt
     2461 tgggtcttaa tatggatcgc tccaaagcgc gggttttcat tgcagcatgg gagtggggac
     2521 catatttggg ggaagaggcg caggagaagg
//
LOCUS       DBNHOH010000204         4787 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1422168, whole genome shotgun sequence.
ACCESSION   DBNHOH010000204 DBNHOH010000000
VERSION     DBNHOH010000204.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4787)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1422168"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /gene="mreC"
                     /locus_tag="AC16V7_03665"
     CDS             <1..637
                     /gene="mreC"
                     /locus_tag="AC16V7_03665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR6256250.1"
                     /translation="NAELEAEVARLQTQIIELQEQLAETNVLSALVDFARANPENRYQ
                     AAAVIARDPSPFLQYVIINRGSDDSLRRGMPVVTQQGLIGRIAAVTAGAARVQLITDP
                     ASTVNVRLEPSGAQAILQGQITGEILLDMIPQGANVQPGDLVLTSGLGGGYPANILIG
                     QISSIRSRDQDIFQRASIQTVLDFSRINIVLVIVNFKPIDINPLLPTPVAP"
     gene            672..1172
                     /locus_tag="AC16V7_03670"
     CDS             672..1172
                     /locus_tag="AC16V7_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256251.1"
                     /translation="MATFIAIPILSGLMILQSGIISRIPLLQGTPDLLMLFILGWAMQ
                     KRVQTAWQWCIIGALIFGLASALPLGTPLLGYGLATGVALLLRRQLWQIPLLAMLVAV
                     LVATFANHAISLIALRLVGNPIPIPQALNLITLPSAFLNLIFAIPAFAMMGDLANWLY
                     PEALEA"
     gene            1169..3244
                     /gene="mrdA"
                     /locus_tag="AC16V7_03675"
     CDS             1169..3244
                     /gene="mrdA"
                     /locus_tag="AC16V7_03675"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HIR6256252.1"
                     /translation="MNESPALHNQVNPWRIWVFALIFLLVLGVYIFRLFTLQIIQGPV
                     WLAQATENRTRAINLPTERGVIYDRNGVVLARNIASYNVVITAANLPDDPGEIQEIFR
                     ALSAQIDVPINLNEISEENPYVPCVSDHGIAQIVEYGESSTPFQPVRIKCDVERITAM
                     VIQEKANDWPGVGIEIVPIRDYPTGVLTASMIGFLGPIPANRAEEFISVGLDPNRDKV
                     GYAGLELQFQDTLAGKNGQRVIEVDVAGKEMRTIGETQAPVPGQNLILTLDTRLQSAA
                     TAIVQREINFWNTYFGEIRSTSAVAIAMNPTTGEILAMVSYPSYENNRMARLIPAYYY
                     NQLVQDPAIPLLNHAVGDTLPAGSVFKLTTAVGALNEGVVTPEQVLETPPQITVSETF
                     SPNEPGRPREFVDWNEAGFGRLDFLGGISNSSNVYFYKLGGGYQDEVPEGLGVCRLGT
                     YARALGYGEYPGTGLPEEERGLIPDPQWKRITRGESWSTGDTYIASVGQGYVLATPLQ
                     VLLSATTIANDGKLMQPTLVREIVDGEGNLVQPFEPNMRWDITQDAIIQEFEETTLRG
                     CEPIEGRTKTVQPWVVAKVQEGMRRAVTDGTLQREFVNATIAAAGKTGTAEYCDKYAL
                     ARNRCIPGEWPTHAWTVAYAPYENPEIAVVAFVYNGGEGASVAGPIVWQLLNAYFELR
                     AIDVAQQAP"
     gene            3268..3948
                     /gene="minC"
                     /locus_tag="AC16V7_03680"
     CDS             3268..3948
                     /gene="minC"
                     /locus_tag="AC16V7_03680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HIR6256253.1"
                     /translation="MQPAVKIKGIREGLLIAIGEGAWDEVSTDLLAKLDEQGTFLKGA
                     RLTIDVGNRVLKAVDLGKMRDALSERGLILWAVLSNSPTTEQTAQSLGMATRLTKPHP
                     EREAKPLDTSVHSGEQAVLIHRTLRSGYSLHFNGHVVVIGDINPGAQVIASGDVVVWG
                     RLRGMVHAGAGGNTDALVCALDLAPTQLRIADQIAITPQRRGKPQPEMARLKDGQVVA
                     ETWNPKKK"
     gene            4019..>4787
                     /gene="minD"
                     /locus_tag="AC16V7_03685"
     CDS             4019..>4787
                     /gene="minD"
                     /locus_tag="AC16V7_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HIR6256254.1"
                     /translation="MAGKVVTVTSGKGGVGKTTATANLGAAMASDGKKVVCVDADIGL
                     RNLDVVLGLENRIVYDLVDVVEGRCRLRQALIRDKRLPELFLIPAAQTRDKSAVSPSD
                     MIRLCDDLRQEFDWILIDSPAGIERGFRNAIAPADIVLVITNPEVSAVRDADRIIGLI
                     EAEEKGPARLVINRIKPDMVRRGDMLSADDVLELLAIELVGLIPEDENVITSTNRGLP
                     IALNGKSRAGQAFRNIAKRLSGEEVPFLEMANDGNLMG"
BASE COUNT         1127 a         1313 c         1292 g         1055 t
ORIGIN      
        1 caatgcagag ctggaggctg aggtagcccg cttgcagaca cagatcatcg agttgcagga
       61 gcaactggct gaaaccaatg tgctctctgc gctagtggat ttcgctcgcg ccaaccctga
      121 aaaccgctat caagccgcag cagtgattgc ccgcgacccc agccccttcc tgcagtatgt
      181 aatcatcaat cgcggctccg atgacagctt gcggcggggc atgcctgtgg tgacacaaca
      241 agggttgatc gggcgcatcg ctgccgtcac agccggtgca gcgcgtgtcc agctcatcac
      301 cgatcctgcc tccacagtca acgttcgcct ggaaccatcc ggagcccaag ctatcttgca
      361 agggcagatc accggtgaga tcttgttgga catgatcccc caaggagcca atgtacaacc
      421 gggcgacttg gtgctcactt ccggcttggg aggcggttac ccggcaaaca tcctcatcgg
      481 acagatctcc agcattcgca gccgggacca agacatcttc caacgcgctt ccatccagac
      541 agttctggat ttctcgcgga ttaacattgt cctggtgatc gtcaacttca agccgatcga
      601 catcaacccg ctcctgccca cacccgtagc accgtaatct ttagaatctg gcttaaaata
      661 gtgtcagtgt catggcaaca tttatcgcaa tacctatcct tagtggatta atgatcctgc
      721 aaagcggaat tatcagccgc atcccgctgc tccaaggcac ccctgacctg cttatgcttt
      781 tcatcctggg gtgggcaatg caaaaacgcg tccaaactgc ctggcagtgg tgcatcatcg
      841 gcgcgctgat tttcggcctg gcctcggctt tgcccttggg tacgccccta ttaggatatg
      901 gactggcaac cggcgtggcg ctgctattgc gcaggcaatt atggcagata cctctactgg
      961 cgatgctggt ggcagtttta gttgccacat ttgccaacca tgccatttcc ttgatcgctt
     1021 tgcgactggt aggaaatccc attcccatcc cccaggcgct gaacctgatc acgctgccga
     1081 gcgcatttct aaaccttatc tttgcaattc cagccttcgc catgatgggt gatctggcga
     1141 actggcttta tcctgaggca ttagaggcat gaacgaatcc cctgccctgc acaaccaagt
     1201 gaacccctgg cgcatctggg ttttcgcgct aatctttttg ctggtactgg gagtgtacat
     1261 cttccgactc ttcacactgc aaatcatcca gggcccggta tggttggcac aggcgacaga
     1321 gaatcgtacc cgcgcgatta acctgccaac cgaacgtggt gtgatatacg accgcaacgg
     1381 tgttgtgctg gcacgcaaca tcgcttccta caatgttgtg attacagctg caaacctgcc
     1441 tgacgacccc ggcgaaatcc aagagatttt ccgcgcgctt tcggcgcaaa tcgatgtccc
     1501 aattaatcta aacgagatca gcgaagaaaa tccctacgtg ccttgtgtat ccgatcacgg
     1561 cattgctcag attgtagagt atggcgaatc atcaacacca tttcaaccag tgcgcatcaa
     1621 gtgcgatgtc gaacgcatca ctgcaatggt cattcaggaa aaggccaacg actggcctgg
     1681 tgtaggcatc gagattgtgc ctatccgcga ttatcccact ggcgtgctca ctgcctcgat
     1741 gattggtttc ctgggaccca tcccagccaa tcgggcggag gaatttattt cggtaggttt
     1801 ggatcccaac cgtgacaagg taggctatgc aggcctcgaa ctgcaattcc aggacacctt
     1861 agcggggaaa aatggacagc gggttatcga ggttgatgta gccggaaaag agatgcgcac
     1921 cattggcgag actcaggcac ccgtccccgg ccaaaatctg attctgactc tcgatacccg
     1981 attacagtct gcggcgactg ccatcgtcca acgggagatc aatttctgga atacatattt
     2041 cggcgaaatc aggtcaacca gcgcggttgc tatcgccatg aacccaacga ccggtgagat
     2101 tctcgctatg gtttcctatc catcctacga gaacaaccga atggcgcgcc tgataccagc
     2161 gtattactat aatcagttgg tgcaagatcc tgccatcccg ctgcttaacc atgcggtggg
     2221 cgacacatta ccagctggct cggtatttaa actgacaacc gcagtcggcg ctttgaacga
     2281 aggcgtggtc acccctgaac aggtgctaga gactccgccc caaatcacag tcagcgagac
     2341 cttctctccc aacgagcctg gtcgcccacg cgaatttgtg gactggaacg aggctggctt
     2401 tggacgtttg gactttttgg gtggcatctc taactccagt aacgtgtatt tctacaagct
     2461 gggtggaggc tatcaggatg aagttcccga agggctgggt gtgtgccgct tgggcacata
     2521 cgcccgcgca ctcggttatg gcgagtatcc tggcactgga ttgccggaag aggagagagg
     2581 cttgatccct gacccacaat ggaaacgcat cacgcgcggc gaaagctggt caacgggtga
     2641 cacctacatc gccagcgtag gtcaggggta tgtactggca actcctctgc aagtgctgct
     2701 ctctgccacc accatcgcaa atgatggcaa gctgatgcaa ccgacgctgg tgcgagaaat
     2761 cgtagatggg gaaggcaatc tcgtccaacc cttcgagcca aacatgagat gggacatcac
     2821 ccaggatgcc atcatccagg agttcgaaga aaccacctta cgaggatgcg agccaattga
     2881 gggacgcacc aagaccgtac aaccgtgggt agttgccaaa gtacaagaag gcatgcgccg
     2941 agccgtgact gatggaactc tgcagagaga gtttgtcaac gcgaccatag ctgcggcggg
     3001 taaaactggc actgctgagt actgcgataa atatgccctg gcgcgcaatc gctgcatccc
     3061 gggcgaatgg cctacccatg cctggacggt cgcctatgct ccttatgaga acccagaaat
     3121 cgccgtggta gcattcgtct ataatggcgg tgagggcgcc agtgtcgccg ggccaattgt
     3181 atggcaattg ctgaatgctt actttgaatt aagagcaatc gatgttgccc agcaggcacc
     3241 gtgagttgat gctataattc ctgcgatatg caaccggcgg ttaaaataaa aggtattcgc
     3301 gaaggcctgc tgatcgctat cggagagggt gcttgggatg aagtgagcac tgatctcctg
     3361 gcaaagctag atgagcaagg taccttctta aaaggcgccc gccttacgat cgacgtgggc
     3421 aaccgggtgc tcaaagcggt tgatttaggc aagatgcgtg acgctctatc tgagcgcggc
     3481 ctgatactgt gggcagtgct gagcaactcg cccaccacag agcaaaccgc acaatccctc
     3541 gggatggcga cccgccttac caaaccacat ccggagcgag aagccaaacc gctggacacc
     3601 agcgtacaca gcggagagca agccgtatta atccaccgca cgctgcgctc gggttacagc
     3661 ctgcatttta atggtcatgt ggtggtcatc ggggatataa accccggcgc gcaggtgatc
     3721 gcatctggcg atgtggttgt ctggggcagg ctgcgcggca tggtccacgc cggggcggga
     3781 ggcaatacag acgcactggt ctgcgcgctg gatttagctc ccactcagct gcgtatcgca
     3841 gatcaaattg ccatcacacc tcagcggcgc ggcaagcctc agcctgaaat ggcacgcctt
     3901 aaggacgggc aggtggttgc agaaacgtgg aaccctaaaa agaagtaacc tgagaagaga
     3961 gggttgaata gctcaatgct ctcattctcc agcatctaat ttatttatcg gagattttat
     4021 ggcagggaaa gttgtcacag taacatctgg aaaaggaggc gtgggcaaaa ccactgccac
     4081 cgccaacttg ggtgccgcta tggcatccga tgggaaaaag gttgtttgcg tcgatgccga
     4141 tatcggtctg cgcaacctgg atgttgtcct aggtttggaa aatcgtattg tttacgatct
     4201 tgttgatgta gtggaaggac gctgccgctt acgccaggca ctgatcagag ataaacgcct
     4261 gcccgaactc tttcttatcc ctgctgccca aactcgcgat aaaagtgcgg tatcacccag
     4321 cgatatgatc cgcctctgcg atgacttgcg acaggaattt gattggatct tgatcgattc
     4381 tcccgccggt atcgaacgcg gctttcgcaa cgccattgcc cctgccgaca tcgtcttggt
     4441 aatcaccaac cccgaggtat cggcagtacg ggatgcggat cgcatcattg gcttgatcga
     4501 ggccgaggaa aaagggccag cacgtctagt gatcaaccgc attaaacccg acatggtcag
     4561 gcgtggggac atgctctctg ccgacgatgt gttggagtta ctcgcgatcg agctggttgg
     4621 cctcatacct gaagatgaaa atgtcatcac cagcaccaat cgtgggttgc cgattgcgct
     4681 taatggcaaa agccgcgcag gacaggcctt ccgcaatatt gccaagcgtc ttagtggtga
     4741 agaagtgccc ttcttggaaa tggcaaatga tggtaactta atggggc
//
LOCUS       DBNHOH010000205         3966 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1037749, whole genome shotgun sequence.
ACCESSION   DBNHOH010000205 DBNHOH010000000
VERSION     DBNHOH010000205.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1037749"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="AC16V7_03690"
     CDS             complement(<1..297)
                     /locus_tag="AC16V7_03690"
                     /inference="COORDINATES: protein motif:HMM:NF024874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6256255.1"
                     /translation="MEIAWYGHSCFRLTERGMATVVTDPYDHAVAGYEVLKLKADIVT
                     VSHDAPGHNFVSAVKGKSHILTGPGEYEIGGVFITGVQTNGYSKRTTDDPRNSLY"
     gene            350..1105
                     /locus_tag="AC16V7_03695"
     CDS             350..1105
                     /locus_tag="AC16V7_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256256.1"
                     /translation="MPVLNPRQTPGSLVIPLPSGGWRLEIPAGPAGRYRLAQLDDYSG
                     LSRRDFPWHPPVSLTIACRASGDTIPGTWGFGFWNDPFSLSLGFGGTNRFPTLPNAAW
                     FFFASPPNYLSVRDDLPAQGGLSATFQSKPWNAALIALGAPLLPLLALPPFARLLRRL
                     GRRIVKQDAANLPIDPMEWHNYQLEWDKNGVTFCLDGRVVLHSSLAPFEPLGLVIWLD
                     NQYAAWPPTGRLSFGTLANSQAAWIELGPVTVS"
     gene            1229..1301
                     /locus_tag="AC16V7_03700"
     tRNA            1229..1301
                     /locus_tag="AC16V7_03700"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1262..1264,aa:Phe,seq:gaa)
     gene            1329..1400
                     /locus_tag="AC16V7_03705"
     tRNA            1329..1400
                     /locus_tag="AC16V7_03705"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1361..1363,aa:Gly,seq:gcc)
     gene            1486..1556
                     /locus_tag="AC16V7_03710"
     tRNA            1486..1556
                     /locus_tag="AC16V7_03710"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1518..1520,aa:Cys,seq:gca)
     gene            complement(1657..>3966)
                     /locus_tag="AC16V7_03715"
     CDS             complement(1657..>3966)
                     /locus_tag="AC16V7_03715"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR6256257.1"
                     /translation="VMLTIYVVTLDDMQRGYAAIDPANIFVQTTLFDDGLVKGIEEVE
                     GVRLAEGVRIFGTRLKASPEQWISINIQSFKNPGQQQINQVRLEEGVWPPGDKEIVLD
                     RYKLHDTNARLGDYLTIETPAGKSRQLKLVGVIQDQSIGAFRSAGGFFNAPVQGYINQ
                     DTLEWLGQPLPDRFDTLYITVAGDSTDTAYLREISASVRDKIEKNNAQVISTSLRSSY
                     EHPNLYLVMAVLAVLVVIGLLVVLLSGFLITNTLQALLNQQVGQIGILKSVGARRVQI
                     VGLYMMLSLFYGLLALAIALPLSFEVAFRIVDFLTIQMNNSFFGFRILPEVILVEAAI
                     AVLMPQIAALVPLWQGTHISVQEALSGIRQDHPLGRSWLDRSISRLRNFSLLLVMSLR
                     NTFRRKGRLALTLVTLTLGGAVFIATFNVRVSLIMYIDRIIQYFLADVNVTLNQPYRM
                     DEVRGLIMEVPGVKMVEGWAFARTELVMADGSVGESVSLLAPPADSPLVKPIIVAGRW
                     IQPGDQNAIALNELFRERYPDLQVGDTIQLQVDGEENDWVVVGFYQMAGKVSGFSAYT
                     SYEYLSELNHQTGKAISYRVVSSWQGLGRAQQEALGRAIEAHLEGAGLDVVDVTTGMS
                     LSETASNGFNVLTAFLLFLALLTAVVGSIGLTGTMSLNVMERTREIGVLRAIGASNRT
                     LMQMVLVEGVLIGFLSWILASLAAFPISVLIAESISQSLFGASSKFGFTPIGFVIWML
                     AVLVLSVLASVIPARNATRLTIREVLAYE"
BASE COUNT          943 a         1139 c          992 g          892 t
ORIGIN      
        1 atagagactg ttgcgtggat cgtctgtcgt gcgcttgctg tacccattgg tctgcacacc
       61 ggtaataaaa acgcccccga tctcatattc gcccggtccg gtaaggatgt gcgacttacc
      121 ctttacggca gagacgaaat tatgcccagg cgcatcgtga ctgactgtta cgatatcggc
      181 tttcaatttc aggacttcat aaccggcaac cgcgtgatcg tatggatctg tgaccactgt
      241 cgccataccg cgctctgtaa gccgaaaaca tgaatgccca taccaagcta tttccataaa
      301 acctccacgt cagcgttgat tatactatag ccaccgttcg gagtagaata tgcccgtgct
      361 aaatcctcgc caaactccag gctcactggt catacccttg ccatccggtg gttggcggct
      421 ggagattccc gccgggccgg cagggcgcta ccggttggcg cagttagacg attattccgg
      481 tctatcacgc cgggatttcc cctggcatcc gcccgtctct cttacgattg cctgccgcgc
      541 ttcaggagat actatcccag gcacctgggg atttggcttt tggaatgatc cattcagcct
      601 ctctctggga tttggcggta caaaccgatt tccgacactg cccaacgccg catggttttt
      661 cttcgcttcc ccccccaact atctctctgt gcgcgatgat ttgcctgctc aaggaggcct
      721 gtcagctacc tttcaatcca aaccctggaa cgcggcttta atcgctttgg gagcgccttt
      781 gctcccctta ctggcactgc ccccctttgc gcgcctgctt cggcgactcg gaagacgcat
      841 cgtgaaacag gatgcagcca atctgccaat cgaccctatg gaatggcaca actaccagct
      901 ggaatgggat aaaaatggcg taaccttctg cttggatggg agggtagtgc tccactcctc
      961 cctggcgcca ttcgagccgc ttgggctggt gatctggttg gacaaccaat acgctgcctg
     1021 gcctccgact ggacgcttga gctttggcac tctggcaaac agccaagccg cctggatcga
     1081 gcttggcccg gtcactgtga gctaaccgtt ctagacctac ccagcatgct tcaggtggct
     1141 gagatataca gcgatccttc ctttgcttga caatacccta cctagtaggt aaaataggcg
     1201 tgctctggga attgagctgt agattcaggc cgagatagct cagttggtag agcacgcgac
     1261 tgaaaatcgc ggtgtcgcca gttcgattct ggctctcggc actctcggcc tataaaatgg
     1321 ccggatacgc gggcgtggtt cagtggtaga acgtctcctt gccaaggaga aggtcgtggg
     1381 ttcgaatccc atcgcccgct ctgcctggtt gaagattttt cgttgccgat tgctgattga
     1441 cgatgatcaa tcagcaatct tcaatctaca atcctcaatt aggttggcga cgtggccaag
     1501 tggtaaggca agggtctgca aaaccctgat cacgggttca aatcccgtcg tcgcctctat
     1561 cagtctgcga cttacgtaag taaaaaataa gagagtgggg caggcaatgc ctgccccact
     1621 ctcttattat tggttgcgtt acttctacaa tttttattat tcgtaagcca acacttcccg
     1681 gatggtcaaa cgggtggcat tgcgggcggg gatgacgctc gccaacaccg aaagcaccaa
     1741 tactgcgagc atccagatca caaacccgat aggcgtaaat ccaaacttgg aagacgcccc
     1801 gaacagggat tgactgatcg attcggcaat taatacgctg atgggaaagg ccgccagcga
     1861 ggccagtatc cagctcagga acccgatcaa caccccctcc accagcacca tttgcatgag
     1921 ggtgcggttg gatgccccaa tcgctcgcag cacaccaatt tcacgcgtgc gctccatgac
     1981 gttaagactc attgtcccgg tcaggccgat gctgcccacc acggcagtca gcagcgccaa
     2041 aaagagcaag aaagccgtga ggacattgaa gccgttggaa gcggtctcgc tcagagacat
     2101 acccgtggtc acatcgacaa catccagacc agcaccctcc agatgcgctt caatcgcccg
     2161 accaagcgcc tcttgctggg cgcgccccag tccctgccag ctcgaaacca ccctgtagct
     2221 gatcgccttg ccggtctggt gattcagctc agagagatat tcgtaactgg tgtaggctga
     2281 aaaaccactc actttaccag ccatttgata gaaacctacc accacccagt cattctcttc
     2341 gccgtccact tgaagctgta ttgtgtcgcc cacctgcaga tcaggatagc gttctcggaa
     2401 caactcgttg agagcgatag cgttctggtc tccaggctgg atccagcgac ctgctacaat
     2461 aattggcttc accagggggc tgtctgccgg gggtgccagc aatgagacac tttcgcctac
     2521 ggaaccatca gccatcacta gctcggtacg ggcaaatgcc cacccttcca ccattttcac
     2581 ccccgggact tccatgatca gcccgcggac ttcatccatg cgatagggtt ggttcagtgt
     2641 gacattgaca tctgccagaa aatactggat gatccggtca atatacatta tcagtgaaac
     2701 gcgcacattg aaagtagcga tgaacaccgc tccccctaag gtgagggtga cgagggtcaa
     2761 agccaggcga cctttgcgac ggaaagtatt gcgtaaagac atgaccagaa gcagcgagaa
     2821 atttcgcaag cggctgatgc tccggtctag ccagctccgg cccagcggat gatcctggcg
     2881 gataccgctc aaggcttctt gcacactgat atgggtgccc tgccacaagg gtaccagcgc
     2941 ggcaatctgc ggcatcagaa cggcaatggc ggcttcaacc aggatgacct ctggaaggat
     3001 acgaaaacca aagaacgagt tgttcatctg aatggtcagg aaatcaacga tgcgaaatgc
     3061 cacctcaaac gagagcggca gggcaatggc cagcgccaga agcccgtaga acaagctcag
     3121 catcatgtag agaccaacaa tttgaacccg ccgggcgcca accgatttca ggataccgat
     3181 ctgtcccact tgctgattca acaaagcctg gagggtgttg gtaatcagaa aaccgctcaa
     3241 caagaccact agcagaccga tcacgaccag cacagccagg accgccatga caagatacaa
     3301 attagggtgc tcataagagc tgcgcagtga agtgctgatc acctgggcat tgtttttctc
     3361 gatcttatcc cgcacggagg ccgatatctc cctcaagtaa gccgtatcgg tgctgtcccc
     3421 tgctaccgta atatataacg tatcgaagcg gtctggtaag ggttggccca gccactccag
     3481 tgtatcttgg ttgatataac cttgcactgg ggcattgaaa aaaccacctg cgctacggaa
     3541 ggctccaatg ctctgatcct gtatcacgcc aaccaacttt agctggcgag atttgcccgc
     3601 gggcgtctcg atggttaaat agtcgcccag gcgagcgttg gtatcatgca atttataacg
     3661 gtcaagaaca atctctttat cgcctggagg ccatacccct tcctctaaac gcacctgatt
     3721 gatttgttgt tgaccaggat tcttaaacga ctggatgttg atcgaaatcc attgctccgg
     3781 gctagctttc aatcgggtgc caaagatgcg cacaccctct gcaaggcgca ctccctcaac
     3841 ctcttcaatg cccttgacaa gcccgtcatc aaacagggtc gtttggacga agatattggc
     3901 aggatcaatg gctgcatagc cgcgttgcat atcatccagc gtcacaacgt agattgtcag
     3961 catcac
//
LOCUS       DBNHOH010000206         2231 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_11830, whole genome shotgun sequence.
ACCESSION   DBNHOH010000206 DBNHOH010000000
VERSION     DBNHOH010000206.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_11830"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2036)
                     /locus_tag="AC16V7_03720"
     CDS             complement(<1..2036)
                     /locus_tag="AC16V7_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR6256258.1"
                     /translation="MLKRFVRIFGGDPNKRAIEKYIETVDAINDLEAEFEALSDEALR
                     AKTQEFRARLAQDETLDELLPEAFATVREASKRTIGLRHYDVQLIGGIALHQGRIAEM
                     RTGEGKTLVATLPLYLNALTGKGTHLITVNDYLARRDARWMAPIYHMLGLSVGVLQMA
                     TRTENGKKAFLVDLEKESPHEDQDQLRMVPRREAYAAEITYGTNSEFGFDYLRDNMTM
                     RLEDRVQRLGDDENTTGLYYAIVDEVDNVLIDEARTPLIISGPAQDEAEWYVRMAQVV
                     KQLRPEDYEVSEKDRNVTLTEIGEVHVEDLLGLPLRDPDRPEDVTHEQARMLGYLEQA
                     LRAQHLFRRNKDYLVQAGKVVIVDEFTGRLMPGRRWSDGLHQAVEAKESVRVQAENVT
                     YATITIQNYFRMYEKLSGMSGTAETEAEEFDKIYTLDVVSIPTNLEYQALREGSPLVE
                     VEDRDEQGYKYLYYTQRDDINKHPVFWKRKDYPDIVYRTAEAKLRAVVREILYNHVRG
                     RPLLVGTTSVELSERVSGRLKAEPLRRLCQVLLLRQVWFDRNDREEDGRLVPELQYLY
                     EPLEQLDISSMRKMARELELHFNPEDSSNIPLLLELLNLDTSDEARLSAALQGGIPHQ
                     VLNARKHTEESQIIAGAGAFGAVTIATNMAGRGVDIKLGGELSEEVVFAASRVLR"
BASE COUNT          418 a          637 c          632 g          544 t
ORIGIN      
        1 cgcaacacgc gactggcggc gaagactacc tcctcggaca gttctcctcc cagcttgata
       61 tctacaccgc gtccagccat gtttgtggcg atagtcaccg ccccaaaagc gcctgccccg
      121 gcgatgatct ggctctcttc agtgtgcttg cgggcgttga gcacctggtg gggaatccca
      181 ccttgtagtg ctgcgctgag acgcgcctca tcgctggtat ccaggttgag tagctccagc
      241 aggagaggga tattcgaaga gtcttccggg ttgaagtgga gttccagttc gcgcgccatc
      301 ttgcgcatgc tggatatatc caattgttcc agcggctcgt acaggtattg cagctctggc
      361 acaagccgac catcttcctc gcggtcattt ctatcgaacc agacttggcg caggagcagc
      421 acctggcaaa ggcggcgcaa cggttcggct ttgagccgtc ctgagacgcg ctcagaaagc
      481 tctaccgagg ttgtgcccac cagtagcggt cgccctctga catggttgta caggatctcg
      541 cgtactactg cccgtagctt ggcttctgct gtgcggtaaa cgatatcggg atagtcttta
      601 cgtttccaga aaaccggatg cttatttatg tcatcgcgct gggtgtaata aagatattta
      661 tagccctgct cgtcccggtc ttccacctcc acgagaggcg aaccctctcg cagcgcctgg
      721 tattccaggt tggtggggat gctaaccacg tcgagcgtgt aaatcttgtc gaactcctcc
      781 gcctcggtct cagcagtacc agacatgcca gatagctttt cgtacatgcg gaagtagttt
      841 tggatggtga tggtggcgta agtgacgttt tcggcctgga cgcgcacgga ctctttggcc
      901 tccacagcct ggtgcaagcc atcagaccag cgccgaccgg gcatcagccg cccggtgaat
      961 tcatcgacga tgaccacctt gcctgcctgt accaggtaat ctttgttgcg gcggaagagg
     1021 tgctgagcgc gcaaggcctg ttccaagtag cccagcatgc gcgcctgctc gtgggtgaca
     1081 tcctccgggc gatcagggtc gcgcagcggc aggcccagca ggtcttctac gtgcacctcg
     1141 ccgatttcag tgagggtgac gttgcggtct ttctcactca cctcgtagtc ttccgggcgc
     1201 agttgcttaa ccacctgagc catgcgcacg taccactcag cctcatcctg tgccgggccg
     1261 gagataatca aaggggtgcg cgcctcgtcg atcaagacgt tatctacctc gtccacgatg
     1321 gcgtaataca agccggttgt gttctcgtca tctcctaaac gctggacgcg gtcctccagg
     1381 cgcatggtca tgttgtcgcg caggtagtcg aagccaaact cgctgttcgt gccgtaggtg
     1441 atttcggcgg cataggcttc ccggcgtggc accatgcgca attgatcctg gtcttcgtgg
     1501 ggtgattcct tttctagatc gacgaggaag gcttttttgc cgttttccgt gcgggtagcc
     1561 atttgcagta cgcccacgct caagccaagc atgtggtaga tgggtgccat ccagcgggcg
     1621 tcgcggcgag ccaggtagtc gtttaccgtg attaaatggg tgcccttacc ggtcagggcg
     1681 ttcagataca gtggcagggt agcgaccagg gtcttgcctt cacccgtgcg catctcggcg
     1741 atcctgccct ggtgcaacgc gatgcccccg atgagctgta catcgtaatg gcgcaagccg
     1801 atggtcctct tgctggcttc gcgcacggtg gcgaaagcct ctggcagcag ttcgtccagc
     1861 gtctcatcct gtgccaggcg ggcgcggaac tcttgggttt tggcgcgcag cgcctcgtcg
     1921 cttaaagcct caaattccgc ctccaggtcg ttgatggcgt cgacagtttc aatatatttc
     1981 tcgatagcgc gtttattggg gtcgccccca aaaatgcgga caaagcgttt gagcatttat
     2041 ttctacctct cgggggtgat tatagcataa ggggcgtcat ctcccagcca tccatttgtt
     2101 atggaaagcg aggacagttg aaagtatttg caccatttgc ttgtcttccc aattgcgtcc
     2161 acagtttacc ggatcgtcct ggcaaaattt taaggttggt taaacctcta gtctgaatcc
     2221 ccatgctata a
//
LOCUS       DBNHOH010000207         1452 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_76102, whole genome shotgun sequence.
ACCESSION   DBNHOH010000207 DBNHOH010000000
VERSION     DBNHOH010000207.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_76102"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="AC16V7_03725"
     CDS             <1..899
                     /locus_tag="AC16V7_03725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6256259.1"
                     /translation="TGAFGYIGKYIARVLLENGERIVTLTNKPAPDDTLREKIPAYPL
                     DFDQPEALVSALSSVDTLYNTYWVRFNYGQTTFDKAIQNTQTLIHAAKKAGVGRIVHI
                     SIANPSTDSNLPYYRGKALLEQFIQQSGLTYAILRPTVVFGLEDVLINNIAFLLRYLP
                     IFAIPGSGNYRLQPIFVEDLAKIAFNAGHSSENQVLDAVGPDIFTYKFLVHLIAEAIG
                     SRSWIIHLPPSMALTLSRLIGLALNDVVLTRDELTGLMADLLVSREPPLGKTSLRDWL
                     ASNAEACGTKYASEIRRHFSSP"
     gene            1049..>1452
                     /locus_tag="AC16V7_03730"
     CDS             1049..>1452
                     /locus_tag="AC16V7_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256260.1"
                     /translation="MQTNLPRGAGETKAAWVLEPFGATPRVAIEAARAGYRVLVAANN
                     PVTRFLLEMAASPPAEDELISALAELAASRRGDERLELHIRSLYHSNCNICSNEVEVE
                     AFLWDRVVLSPQAEKPSPSGPVPFAKIYHCPH"
BASE COUNT          346 a          393 c          316 g          397 t
ORIGIN      
        1 tcactggagc tttcggttat attggcaaat acatcgctcg cgttctactt gaaaatggcg
       61 agcgcattgt cacattgacc aataaacccg cccctgacga tacccttaga gaaaaaatcc
      121 cagcttaccc cttagatttc gaccagcctg aggcgctagt ttcagcactc tccagtgtgg
      181 acacattgta caacacctat tgggtgcgtt ttaattatgg tcaaacgact tttgacaaag
      241 ctattcaaaa cacccagacc ctgatccacg ctgccaaaaa agctggtgtg gggcgcattg
      301 tgcacattag catcgccaac ccttcgactg attcaaatct cccctattac cgcggtaaag
      361 cacttctgga acaatttatc cagcaatctg gtctaacata tgctatcctt cgaccaacag
      421 ttgtttttgg tcttgaagat gtcctcatca acaacattgc tttcctattg cgctacttac
      481 caattttcgc tattcctggc tcgggcaatt accgcttaca gcctatcttc gttgaagatc
      541 tggcaaaaat tgcttttaac gctggccata gcagcgaaaa tcaggtattg gatgctgtcg
      601 gacctgatat attcacctat aaattcttgg tgcacctgat cgcggaagcc attggcagcc
      661 gttcgtggat catccatcta ccacccagca tggcgcttac actatctcga ctgattggcc
      721 tcgccttaaa cgatgttgtc ctgacacgag acgaactaac agggctgatg gctgatttat
      781 tggtttcccg cgagccacct ctaggcaaga catctttgcg cgattggctg gcatccaatg
      841 cggaagcatg tggcactaaa tacgcatcgg aaatccgtcg tcatttctca tcaccgtaaa
      901 gccagttgta tttttttacg aaatctatat actatccata gaaactgcta tgtctatgat
      961 cccttatctg gttgggcttc ctcctgccaa gccagaacct ttgtcccgtt acttgcctcc
     1021 aattcccgta ggggtggtta gaacttggtt gcaaacaaat ttgccgcgag gcgctggcga
     1081 aaccaaggct gcctgggtgc tggagccgtt tggggctact ccaagggttg ccatcgaggc
     1141 tgcgcgtgct ggatatcgtg tattggtcgc tgccaataac ccagtcactc gcttccttct
     1201 agaaatggca gccagtcccc ccgctgagga tgagctgatc tccgctctgg ccgagctggc
     1261 cgcctcacgc agaggtgatg agcgtctgga gcttcatatt cgctccttat accattccaa
     1321 ttgcaatata tgctcaaatg aagtcgaggt agaagcattt ttatgggatc gcgttgtctt
     1381 atctccccag gcagagaaac cctcaccttc agggccagta ccatttgcca aaatctatca
     1441 ctgccctcat tg
//
LOCUS       DBNHOH010000208         1790 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_203999, whole genome shotgun sequence.
ACCESSION   DBNHOH010000208 DBNHOH010000000
VERSION     DBNHOH010000208.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_203999"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..1151
                     /locus_tag="AC16V7_03735"
     CDS             204..1151
                     /locus_tag="AC16V7_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256261.1"
                     /translation="MKTTWLDEADQEQEAQKALLKAFSGTLHHFFGGWRELFKGVRDS
                     RNPELIRYALEGLLSTGVLMYLFQLGARRQINYQLRGNAPSQAKFEAWFEVDEVPHGD
                     TLNYAFRRVVPAEVQEVVCCMVATLICKKVLYRWRLYGNFLLVIDGTRVLTFQARDCR
                     YCLTQKLHNGEKLYYHPVLEAKLVTANGFAFSLMTEFIENTDPQADKQDCKLKAFYRL
                     SRRVKARFPQLPICLLLDGLFAGGPTFQLCQDYDWRSMITLQDADLPQVHRTYELVVR
                     SQTPQDHKQIRLGRSSRSHRSIIGSGRSIIAIARIATTR"
     gene            1148..1732
                     /locus_tag="AC16V7_03740"
     CDS             1148..1732
                     /locus_tag="AC16V7_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256262.1"
                     /translation="MNVVTCKESTPTRQGDISTTTFKWLTNFIPTAHNVDTLANQTGR
                     LRWKVENEGFNIQKNGGFNLQHPYSQDDNARKVFYYLLQIAHLLFQLLEKGSLFRQAF
                     PRGFGSLKNIAFRLLEAWRNLRLIPRLSVPSKAVATRSALTPPETSGMPAHSRCSACE
                     FGLYQAEPLCEDDMPCFLFYPTLFPSCFFPADIL"
BASE COUNT          410 a          487 c          474 g          419 t
ORIGIN      
        1 ggtcgtagcc gtggtagacg gttgccatgc ccccgcgccc cagctcggct ttgatctcgt
       61 aacgtcctat tttttccggt gccataatag atcaagtata cctatcccaa tggtggaacg
      121 caagaacaat tactagaaaa cagcggtgaa gaagcgaagg tttttcgcac aagatttttc
      181 gcacaagggg gtaaggttgg gggatgaaga caacctggtt agatgaggca gatcaagagc
      241 aagaggctca aaaagccttg ttgaaagcct tcagtggcac gctgcatcat ttctttggcg
      301 gctggcggga actattcaag ggggtcaggg atagtcgcaa tcccgagctg atcagatatg
      361 cgctggaagg tttactgagc acaggcgtgc tgatgtattt gtttcaattg ggggcgcggc
      421 gacagatcaa ctaccaactg cgtggcaatg caccatcgca agccaaattc gaagcctggt
      481 tcgaggtaga tgaggtgcca catggagaca ccctcaacta tgcctttcga agagtggtac
      541 cggcggaagt gcaggaagtg gtatgttgca tggtagcgac cctgatctgc aagaaggttc
      601 tgtatcgctg gcggctgtat ggcaattttc tgctggtgat cgatggcacc agagtgttga
      661 cctttcaggc ccgagactgc cgctattgtc tgacgcagaa actccacaac ggggagaagc
      721 tgtactatca tccggtgctg gaagccaaac tggtcacggc caatggcttt gcattttccc
      781 tgatgaccga gttcatcgag aatacggatc cacaagcgga caagcaagat tgcaagttaa
      841 aggctttcta tcggttgagc aggcgggtga aggctcggtt tcctcagttg ccgatctgcc
      901 tgttgctgga tgggctgttt gcaggcgggc caaccttcca actctgtcag gactacgact
      961 ggcgctccat gatcaccctg caggatgcag atctgcctca ggttcaccgc acctatgagc
     1021 tggtggtgcg ctcccagacc ccgcaagacc ataaacagat ccgcctgggc cgcagcagca
     1081 gatcacacag gagtatcatt gggtccggca gatcgattat cgcgatagcc aggatcgcca
     1141 ccacacgttg aacgtagtga catgcaaaga aagcacacca actcgccagg gagatatcag
     1201 caccactacc ttcaaatggc tgaccaactt catccccacc gcccacaatg tcgacacgct
     1261 ggccaatcag acgggaaggc tgcggtggaa ggtcgagaat gaaggtttca acatccagaa
     1321 aaatggcggt ttcaacctgc aacaccccta cagccaggac gacaatgccc gcaaagtctt
     1381 ctactacctg ctccagatcg ctcacttgct cttccagttg ctcgaaaaag gcagcctgtt
     1441 tcgccaggct tttccccgcg gcttcggctc gctcaagaat atcgcttttc gcttgttgga
     1501 agcctggcgc aacttgcggc tgatcccgag gctttctgtg ccctctaaag cggtcgctac
     1561 cagatccgct ttgactcctc ctgaaacaag cggcatgcca gcccactccc gctgctctgc
     1621 ctgtgagttt ggcctctacc aggcagaacc tctatgtgaa gatgacatgc cgtgcttctt
     1681 attttatcct accctgtttc cttcttgttt tttccctgct gatatcttat gaggccaccc
     1741 ctgcccattt tggggagctg gtttcgcgcc tgttcttgct cgtccagtcc
//
LOCUS       DBNHOH010000209         1568 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_139828, whole genome shotgun sequence.
ACCESSION   DBNHOH010000209 DBNHOH010000000
VERSION     DBNHOH010000209.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_139828"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1563)
                     /locus_tag="AC16V7_03745"
     CDS             complement(<1..1563)
                     /locus_tag="AC16V7_03745"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR6256263.1"
                     /translation="MPITDNLIAKVLQTRKPVRTITQTAEDPLIKLSTGFLVRSLLHV
                     PIISRGKALGVLTMVNHSHQKPFLDKDESLMTSLASYASIALDNAGLYHRAQQEIIER
                     RKIEEALRESEERYALAMRGSNDGLWDWDLRSNEIYFSPRWKSMLGYSEKEITNSPQE
                     WFSRVHPDDIDKLRIDIETHLTRNSPHFENEHRLQHKDGDYRYILSRGIAVSDANGMA
                     QRIAGSITDITERKAVEQKLLQYAFYDKLTGLPNRALFVDHLSLAIERAKRRPDFQFA
                     VLFMDLDTFKDVNDSLGHMVGDELLVSVARLLQSRMRSTDTVARFGGDEFVILLDDIH
                     DKFNAQQVSEWIQSALAEPFILNGQEVYISASIGIVLSETGYQRSEEVLRDADIAMYN
                     AKANGKSRFEIFNPEMRTRLLDRLELANDLRRAIENQELRVHYQLIVSLNNGRITGLE
                     ALVRWQHPQRGLLAPIEFIPLAEDTGLIIALDRWVLREACRQMHSWKLENPMFSDLTI
                     SVNISGKQLSQGD"
BASE COUNT          407 a          367 c          369 g          425 t
ORIGIN      
        1 gtccccttga gatagctgct tgccagagat gttcacgcta atggttagat cagaaaacat
       61 ggggttttca agtttccaag agtgcatctg gcggcaagcc tcacgcaaga cccatcggtc
      121 gagggcgata atcaagccag tatcttcagc taatggaata aattcgattg gagcaagcaa
      181 gcctcgctgc gggtgctgcc aacgaaccaa tgcttcgaga cctgtgattc gaccgttatt
      241 caaggaaacg atgagctgat aatggacgcg caattcttga ttctcaattg cccttcgcaa
      301 atcattggct agttccaacc gatccaataa acgagtgcgc atttcggggt tgaaaatctc
      361 aaacctagat ttgccgttgg ctttggcgtt gtacatggcg atatcggcat cgcgcaagac
      421 ttcttcggag cgctggtaac ctgtttcgct gagtacaatc ccaatactgg cggagatgta
      481 aacctcctgt ccattcaaga tgaatggctc agcgagggct gactgaatcc actcggacac
      541 ttgttgggcg ttgaatttat cgtggatatc gtcaagcaga ataacaaatt catccccacc
      601 gaaacgagca acagtgtctg tggaacgcat acgtgattgc aataagcgtg caacagagac
      661 aagtagttca tcgccgacca tgtgccccag gctatcgttt acgtctttaa atgtgtctaa
      721 atccatgaac agaactgcaa attgaaaatc aggccgtcgc ttagcccgct cgattgccaa
      781 actcaggtgg tcaacgaata acgctcgatt tggaagtcct gtgagcttat cataaaaggc
      841 atactggaga agtttctgtt cgacagcttt tctttcggtt atatctgtga ttgatccggc
      901 aatacgctga gccatcccat tggcgtctga aacggctatc ccacggctaa gtatatagcg
      961 ataatcccca tctttgtgtt gtagtcggtg ctcattttca aaatgaggtg aatttctagt
     1021 taagtgggtt tcgatatcta tgcgtagttt atcgatatcg tcgggatgaa cacgagaaaa
     1081 ccattcttga gggctattag tgatctcttt ttcactgtac cccagcatag atttccaacg
     1141 cggtgagaaa tatatctcat tcgaacggag atcccaatcc cacaagccat catttgaacc
     1201 acgcatcgcc agcgcgtatc gttcctcgct ctcgcgcaat gcttcttcta tttttcgtcg
     1261 ctcaatgatc tcttgttgcg ctcgatgata caaaccggcg ttgtccaacg caattgaggc
     1321 ataactcgcc agagatgtca ttagggattc atccttatcc agaaatggtt tttgatggct
     1381 gtgattaacc attgtaagga cacccagcgc ttttccgcgt gaaataatgg gtacatggag
     1441 tagactgcgg acgagaaatc ctgtactcaa tttgatcaaa gggtcttcag cagtttgcgt
     1501 gatggtgcga acaggctttc tggtctgcag cactttggcg atcagattat cagtaatcgg
     1561 caagcgca
//
LOCUS       DBNHOH010000210         1512 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_204057, whole genome shotgun sequence.
ACCESSION   DBNHOH010000210 DBNHOH010000000
VERSION     DBNHOH010000210.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_204057"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="AC16V7_03750"
     CDS             <1..1215
                     /locus_tag="AC16V7_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohexomutase domain-containing protein"
                     /protein_id="HIR6256264.1"
                     /translation="ANGLKVYLTDGATPTPVIAYSIVNKGAVGGVNITASHNPATDNG
                     FKVRNEFGGAIAPEGLKEIEGRIPDSEEKVRRVRLDDAISDGRIVRFDPTPAYIEQIH
                     SLIDLQPIKDAGLKVMVDPMWGNGAGWFQNLLGGGKTQILEIHSQRNPLFPEMSRPEP
                     IPPNVNAGLAAAYEQGADALIITDGDADRIGVGDENGQFIDQLRVYALLAYYLLEVRG
                     ERGPIVKTLSTTNMLNKLGKLYNVPVYDVGVGFKYVAPKMLETHAMIGGEESGGYAFH
                     GHVPERDGILAGLYILDMMIKLDKKPSELLDLLFSKIGAHYYDRIDTRFEGDRQSREK
                     MILSASPKAIGGLEVTGLETTDGFKFGLEDGGWLLIRFSGTEPIIRVYTETTHKDRVQ
                     DILRDGLRIVGL"
     gene            1219..>1512
                     /locus_tag="AC16V7_03755"
     CDS             1219..>1512
                     /locus_tag="AC16V7_03755"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR6256265.1"
                     /translation="MFFLADTHCHLDFDTFDSDRDAVIQRAQNVGVGRMLNPGIDIQS
                     SRVALRLAREYDEVYVACGVHPNDALTWDNQSQNELLGMLKQEKVVAVGEIGLD"
BASE COUNT          393 a          312 c          426 g          381 t
ORIGIN      
        1 gccaatggcc tgaaggtata tttaactgat ggagcgacac caacgccggt gatagcctat
       61 tctattgtca acaaaggtgc agtgggcggg gtcaatatta cagcatctca taacccagcg
      121 acagacaatg gttttaaggt ccggaacgaa tttgggggcg caattgcgcc agaaggtctt
      181 aaagagattg aaggtcggat accagattct gaagagaaag tcagaagggt gaggttggat
      241 gatgccatca gtgacggccg aatcgttcgc tttgacccaa ccccggcgta tatcgagcaa
      301 attcatagcc tgattgatct acagccgatt aaagacgctg gtttgaaggt aatggtggac
      361 ccaatgtggg gcaacggagc aggttggttt caaaatctat tgggaggagg aaaaacccag
      421 attctcgaga tacatagtca gcgtaatcct ttatttccag agatgtcccg tccagagcct
      481 ataccaccga atgtcaacgc gggcttagcg gctgcgtatg agcagggtgc tgacgcattg
      541 atcatcacag atggcgatgc tgatcgaatt ggagtaggcg acgagaatgg acagttcatt
      601 gaccaactgc gtgtttacgc cttgttagcc tattatttat tggaagtgcg cggtgagcga
      661 ggcccgatcg tcaaaacgct ttccacaact aatatgctca acaagcttgg gaagctttac
      721 aatgttccgg tctatgatgt tggtgtagga tttaagtatg tagccccgaa aatgttggaa
      781 actcatgcca tgatcggtgg tgaggaatca ggcggttacg ccttccatgg tcatgtaccc
      841 gagcgggatg gaattctagc tggtctgtac atactggata tgatgattaa gctggataag
      901 aagccctctg agttgttaga tttgctcttc agtaaaattg gcgcacatta ttatgaccga
      961 attgatacgc gtttcgaggg agatcgtcaa tcacgtgaaa agatgatctt aagcgcatcc
     1021 cctaaagcca tcggcgggct ggaagtgact ggtttagaga caactgatgg cttcaaattt
     1081 ggcctggaag acggcggttg gctgcttatc cgtttttctg ggacagaacc aatcatccgt
     1141 gtttacaccg agaccacgca caaagatcgc gtgcaagata ttttgagaga tggtctgcgc
     1201 atcgttgggc tgtaaacggt gttctttcta gcagatactc attgccattt ggactttgac
     1261 acattcgata gtgatcgcga tgcagtcatc cagcgagcgc agaatgtggg ggttgggcgc
     1321 atgcttaacc cggggattga tatccagtcg agtagggtcg cattgcgcct agctcgtgag
     1381 tatgatgagg tgtatgtagc ctgtggcgtg caccccaatg acgcgctaac ttgggacaat
     1441 caatcgcaaa atgagctgtt gggcatgctt aagcaggaaa aggttgtcgc tgtcggagaa
     1501 atcgggctag at
//
LOCUS       DBNHOH010000211         2044 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_396304, whole genome shotgun sequence.
ACCESSION   DBNHOH010000211 DBNHOH010000000
VERSION     DBNHOH010000211.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_396304"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="AC16V7_03760"
     CDS             <1..299
                     /locus_tag="AC16V7_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256266.1"
                     /translation="LKEEFGLPVVERPIDRTEVYLCDELFMTGTAAQITTVVKVDNRP
                     LGSGVMGPITTKLRKAFDDAIRGRNPRFRHWNVPVYPKPSTQEQKSTVSEKLAY"
     gene            complement(350..1735)
                     /locus_tag="AC16V7_03765"
     CDS             complement(350..1735)
                     /locus_tag="AC16V7_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR6256267.1"
                     /translation="MDLTFLTISQAADLISRRELSPVELAKAYFDRIRQFEPLINSYI
                     TVTEETALAAARQAEGEIQKGNYRGLLHGIPLALKDLYETAGVRTTHGSKVYADYVPE
                     SDGVVVKKLKAEGVVMLGKTNMHEIALGVTNVNPHFGPCRNPWSLERVTGGSSGGSAA
                     ALAAGMCMGSFGSDTGGSIRIPASLCGIVGLKPTFGRISLQGVIPLSWNLDHAGPMAR
                     SVRDAAILLAATAGYDPDDPASIDVPTQDYLAQLENGVRGWKIALAVDEFFSRVDDEV
                     NAALQEAAQVFKELGAQVVPVEFPEGYAAAAANGGIVTSDAAAFHIERIQTRSEDFGA
                     DVLERMQGGAARTSSEYARLRREQSRLRRVYERFFEGYDILLTATTPVTAPPIEGPNA
                     IEQARLLTRFTAPFNLTGLPALSLPCGYSSEGLPVGLQIISRPWAEASVLRAGTAYEL
                     ATQWQQHRPAL"
     gene            complement(1746..>2044)
                     /locus_tag="AC16V7_03770"
     CDS             complement(1746..>2044)
                     /locus_tag="AC16V7_03770"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HIR6256268.1"
                     /translation="KVALLSNAWDNMRDILENEWKIASDFDEVIISAEIGLAKPDHRI
                     YQLTLSRLGVGADEAVFVDDFVENVDAARWLGMRAIHFRSPDQARAELERMLNT"
BASE COUNT          465 a          603 c          531 g          445 t
ORIGIN      
        1 ttttgaagga ggaattcggt ctgccggtgg tggaacgccc catcgaccgg acagaagtct
       61 acctttgtga cgagctattc atgaccggta ccgcggctca aatcacaacc gtggttaaag
      121 tagataatcg gccattgggc tccggtgtca tgggaccgat caccaccaag ctgcggaaag
      181 cctttgacga cgcaatacgc gggcggaacc cccgcttccg ccattggaac gtgcctgttt
      241 atcccaagcc gtctactcaa gaacaaaaat caacagtcag cgagaagctg gcttattgat
      301 gaaaaggaca cgccccaggc tcatctgggg cgtgtccttt tcatccaact tacagcgccg
      361 gtcggtgttg ctgccattgc gttgcaagct cataagccgt tccagcgcgc agcacagatg
      421 cttcagccca tggacgggag atgatctgca atccaaccgg taagccctcg gatgaataac
      481 cgcatggtaa agatagggca ggcaagccgg tgaggttgaa tggcgctgtg aaacgggtta
      541 atagtcgcgc ttgctcaatt gcattaggcc cctcgattgg aggcgctgtc acgggagtgg
      601 tcgccgtgag caggatatcg tagccttcga agaagcgctc gtagacacgc cgcaatcgac
      661 tttgctcgcg gcgcaaacgg gcatattcag aagatgtgcg cgctgctcca ccttgcatgc
      721 gctcgaggac atccgctcca aaatcctcgg agcgggtttg aatacgctca atatgaaatg
      781 ctgccgcatc gctggtcaca atccccccat ttgctgccgc ggctgcgtac ccctctggaa
      841 actccaccgg cacaacttga gcgccaagtt ctttgaatac ctgcgcagcc tcttgtagcg
      901 ctgcgttcac ctcatcatca acgcggctaa aaaattcatc tactgccaga gcgatcttcc
      961 agcctcgaac tccattctct agctgcgcta aataatcctg cgtggggaca tcgatagagg
     1021 ctggatcatc cgggtcatag ccggcagtag ctgccaggag gattgcagca tcgcgcacgc
     1081 tgcgcgccat tggaccagcg tgatccaagt tccagctgag cgggatgaca ccctgcaggc
     1141 taatcctgcc aaaggtcggc ttgagcccca caattccgca cagagaggct gggatgcgga
     1201 tcgatccacc cgtatcgctg ccaaaagatc ccatgcacat cccagcggct aatgccgccg
     1261 cagagccacc cgatgagcca ccggtgactc gctccaaact ccacgggttc cggcacggtc
     1321 caaagtgagg gtttacattg gtcactccga gggcgatctc gtgcatattg gtcttgccca
     1381 acatgacgac cccctctgct tttagctttt tgacgacgac accatcggac tcagggacat
     1441 aatccgcgta gaccttcgac ccatgggttg tccgcacacc agccgtctcg tacaggtctt
     1501 tcagcgccag cgggatgccg tgaagcagtc cgcgatagtt acccttttgg atttcgcctt
     1561 ctgcttggcg ggctgctgcc aatgccgtct cctcagtgac ggtgatatag ctatttatga
     1621 gcggctcaaa ttggcggata cggtcaaaat atgcctttgc aagctcgacc ggggagagct
     1681 cccgccgcga gatcaggtcg gcagcctggc tgatggtaag gaaagtgaga tccatgatat
     1741 cctctttagg tatttaacat gcgctccaat tccgcccgcg cttgatccgg cgagcgaaaa
     1801 tggatagcgc gcatcccaag ccagcgcgct gcgtccacgt tttccacaaa atcatctacg
     1861 aaaacagctt catccgcccc cacacccaag cggctcaatg taagctggta gatgcgatgg
     1921 tcaggtttag ccagcccaat ctctgctgaa attattacct catcaaagtc gctggcgatc
     1981 ttccattcat tttccagtat atcccgcata ttatcccagg cattgctcaa caaggcaacc
     2041 ttgt
//
LOCUS       DBNHOH010000212         2395 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1678892, whole genome shotgun sequence.
ACCESSION   DBNHOH010000212 DBNHOH010000000
VERSION     DBNHOH010000212.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1678892"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="AC16V7_03775"
     CDS             complement(<1..283)
                     /locus_tag="AC16V7_03775"
                     /inference="COORDINATES: protein motif:HMM:NF014665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HIR6256269.1"
                     /translation="MLQKFSRYPYIVLTALLGVFILIVMGGVLSATGAGGACPDWPTC
                     FGVWTPPPASLEQPLADYLHRFGTALATFFILAAVWRGKRSQPWIIRPLL"
     gene            complement(335..1129)
                     /locus_tag="AC16V7_03780"
     CDS             complement(335..1129)
                     /locus_tag="AC16V7_03780"
                     /inference="COORDINATES: protein motif:HMM:NF014193.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial respiratory chain
                     complex IV assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HIR6256270.1"
                     /translation="MSGHAGSLVVHKKGVFQALRVFSRRWILSTLLVLAAAAVLARLG
                     LWQLDRLEQRRAFNARVQAQISQPVLELTAGTMGLDLASMEYRAVHLRGEYDFSHQVA
                     LRNQVWGNEPGVRLLTPLKIEGTDSYILIERGWVPGLESTPQDWQQYDETGMVEINGM
                     IRASQSKPDFGRRADPVPAPGEEPLRLWHFANVERIADQVPYRLLAVYIQQAPSLALT
                     GLPNRSLPELELTEGPHMGYALQWFSFAVLLLIGYPFYVNKRELKR"
     gene            complement(1129..1653)
                     /locus_tag="AC16V7_03785"
     CDS             complement(1129..1653)
                     /locus_tag="AC16V7_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256271.1"
                     /translation="MNAKFDVGPYIIRFIISALISVVVAGLINEGAYLFQKEKYDRPP
                     KTIQLVVPNGTADQLAAGEPIKSIPENMVFVAGDVLEVKNEDSVAHQLGPVWVPAGAT
                     GRLDLNDPNKYAYSCSFTTQRYLGLDVRQPTTLGTRLTALAVGGPPLLVFFFIYSLLV
                     FPVKPAQKEAVGQA"
     gene            complement(1653..2252)
                     /locus_tag="AC16V7_03790"
     CDS             complement(1653..2252)
                     /locus_tag="AC16V7_03790"
                     /inference="COORDINATES: protein motif:HMM:NF014667.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR6256272.1"
                     /translation="MRKNPLFYALYGLAALALITLGWRIFAGPYKFKGSQIDPPVPAA
                     DFTLTDQNSQAFRLSEQRGKVALIFFGYTNCPDVCPITLTTYTKIRARLGDEADKVRF
                     IFITVDPERDTVEQIRSHLGNFDPALIGLTGSREELESVWKNYGVFQSQHGAEGVEHY
                     DVDHSSRIYAIDPQGNWRLTYPAEMDYETIAKDVEHLID"
     gene            complement(2260..>2395)
                     /locus_tag="AC16V7_03795"
     CDS             complement(2260..>2395)
                     /locus_tag="AC16V7_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HIR6256273.1"
                     /translation="NGRKGLYSAERHWAVEACAVYWHFVDVVWIFFYPALYLIGLPVI
                     "
BASE COUNT          577 a          712 c          630 g          476 t
ORIGIN      
        1 cgagcaaggg acggatgatc cagggctggc tgcgcttacc tcgccagact gccgccagga
       61 tgaagaacgt agccagagcc gtcccgaaac ggtgcagata atccgccaac ggttgttcta
      121 acgaggcagg gggaggtgtc cacaccccaa aacaggtggg ccagtctgga caggcgcccc
      181 cagcgcccgt ggcgctcagc acgcctccca tgacaatcag gataaagacg ccaagcaagg
      241 cggtcaggac aatatatggg tagcgagaga atttttgaag cataatacct tgtgtgcaac
      301 gcgctcggtt aatcaaggaa tacccgggag caggttagcg cttaagctcc cttttattca
      361 cgtaaaatgg atacccaatc agcaacaaga cagcaaagct gaaccattgc aatgcgtaac
      421 ccatatgcgg cccttccgtc aattccagct ctggcaggct gcgattcggc agacccgtca
      481 aggctagaga aggcgcctgc tggatataaa ccgccaacag ccgatagggc acctgatcgg
      541 cgatacgctc cacgttggcg aaatgccaca ggcgcaaggg ctcctcgccg ggtgccggta
      601 cgggatcagc gcggcgaccg aagtccggct tgctttgcga agcgcggatc atcccgttaa
      661 tttcgaccat gccagtttca tcatactgct gccaatcctg gggggttgat tcaagcccag
      721 gcacccagcc gcgttcgatg agaatatagc tatcagtgcc ctcaattttt aatggggtga
      781 gcaggcgtac accaggctca ttgccccaca cctggtttcg caaagctacc tggtgagaga
      841 aatcgtactc gccgcgcagg tgtacagctc ggtattccat acttgccaga tccagcccca
      901 ttgtgccagc ggtcaattcc aataccggct ggcttatttg tgcctgaaca cgggcgttga
      961 atgcgcggcg ctgctccaga cggtcaagct gccagaggcc caggcgagcc agcaccgccg
     1021 ccgcggccaa aaccagcagc gttgaaagaa tccagcggcg actgaatacg cgcaaggctt
     1081 gaaatacacc tttcttatgc accaccagac tgccagcatg cccgctcatc acgcctgccc
     1141 cactgcctct ttctgagctg gcttgactgg aaataccagc agactataga tgaaaaagaa
     1201 caccaataag ggaggaccac ccaccgccaa ggctgtcagg cgtgtcccca gggtggttgg
     1261 ctgtcgcacg tccaacccca gataccgttg cgtggtaaag gaacaggaat aagcgtactt
     1321 attgggatcg ttcagatcca agcgcccggt agcgccggct ggcacccaga caggcccaag
     1381 ctggtgcgct acgctatctt cattcttgac ctccagcaca tcacccgcca caaagaccat
     1441 attctccggg atcgatttga tgggctctcc cgcagccaac tggtctgccg tcccattggg
     1501 gacgacgagc tggatggtct tcggcggccg gtcatatttc tctttctgga acaagtaagc
     1561 tccctcatta atgagcccag ccactaccac cgagatcagc gcggagataa taaagcggat
     1621 gatataggga ccaacatcaa actttgcgtt catcaatcga tcaaatgctc cacatccttg
     1681 gcgatggttt cataatccat ctcagccgga tacgtcaatc gccaattgcc ttgcggatcg
     1741 atggcataga tgcgcgacga gtgatccaca tcgtaatgct ccaccccctc ggctccatgc
     1801 tgggactgga acacgccgta attcttccag acgctctcaa gttcttcccg gctgccggtc
     1861 agaccgataa gcgccgggtc aaaattcccc aggtgcgagc ggatctgctc taccgtatca
     1921 cgttcaggat ctacggtgat aaagataaag cgcactttgt ctgcctcatc gcccaagcga
     1981 gcgcgtattt tggtatatgt cgtgagcgtg attgggcaga catccgggca attggtgtac
     2041 ccaaagaaga tcaacgccac ttttccacgc tgctcgctca accggaatgc ctgactgttt
     2101 tgatccgtca gtgtgaagtc tgcggctggc acaggcgggt cgatctgcga gcccttgaac
     2161 ttgtacgggc cagcgaaaat gcgccagcca agcgtgatca gcgccagcgc agccagtcca
     2221 tacaatgcgt aaaaaagtgg gttctttctc acttcgctct caaataaccg gcaacccaat
     2281 caggtacaaa gccgggtaga agaaaatcca caccacgtcg acaaagtgcc agtacacggc
     2341 gcaggcttcc accgcccaat ggcgctcggc tgagtagagc cccttgcgcc cattg
//
LOCUS       DBNHOH010000213         1701 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_909774, whole genome shotgun sequence.
ACCESSION   DBNHOH010000213 DBNHOH010000000
VERSION     DBNHOH010000213.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_909774"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="AC16V7_03800"
     CDS             <1..340
                     /locus_tag="AC16V7_03800"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6256274.1"
                     /translation="TQDGLPAGCVCLKGNDATTCELKRLYVRPSFRGQNIGWQMVNLL
                     VDEACQSGYQRIVLDRHIAMKKAHAIYQAVGFQIVEAPDDFPQYLRPTVVFMECDLST
                     NCERNGAGID"
     gene            556..1452
                     /locus_tag="AC16V7_03805"
     CDS             556..1452
                     /locus_tag="AC16V7_03805"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6256275.1"
                     /translation="MNQTPDSKIYAAFVLTMLIGGANFIAVSLSNRELPPLFGATLRF
                     ALATFLFFLIARAWRIPLARGRDAAGAVIYGLLNVGAAYALLYYALVSLNAGTVAVIV
                     ASVPLFTLAIAVLFGQERLSFNRVAGGILAIAGIAILRGFGGDFVLSYFVAAVVGAVA
                     IAASSVVAKAYPNVHPINMNAIGTAAGTLLLAVSSLVFGEQWVLPHENTTWFAVGWLV
                     IAGSVGLFQLFLFVIKRWTASATVYALTGMPVIATALGIIILDQPVTMEVLAGGALVI
                     AAVYVGAIARQRKTSKFYRQKA"
BASE COUNT          381 a          415 c          446 g          459 t
ORIGIN      
        1 cacacaagac ggactgccag cagggtgtgt atgtctcaaa ggcaatgacg ccacaacttg
       61 cgaactcaaa cggctctatg tgcgcccttc ctttcgggga caaaacatcg gctggcaaat
      121 ggttaacttg ttggtggatg aggcatgcca atctggatat caacgaatag tgctggacag
      181 acatatcgcg atgaaaaaag cgcatgcgat ctatcaagct gtgggctttc agatcgtgga
      241 ggctcccgat gattttccac agtacctgag gccaactgtg gtattcatgg aatgcgactt
      301 atccacaaac tgtgagagaa atggtgcagg tatagattaa taactggact aatcaattcg
      361 aagtgcggcc caaccacgtg tggtgtggac ggcagagatt atgcggtgct tgctggtatt
      421 ttctggattt gagttattat tgctcccaag cagaatccac gtccgcctgc cagcaggtaa
      481 cgcaaagcat taggcaggtc aggaattcca tttttatgtc ggtattgcat tattgtatcg
      541 gaaggatggt taattatgaa tcaaacaccc gacagtaaaa tctacgccgc attcgttctc
      601 actatgctta ttggaggtgc taactttatt gcagtcagcc tgagcaacag agaactgccg
      661 ccactcttcg gtgcaacctt gcgttttgcc ctggctactt tccttttctt tttgattgcc
      721 cgtgcttggc gtatcccctt ggcacgcggt cgggacgcag caggggctgt gatctatggg
      781 cttcttaatg taggcgctgc gtatgcactc ttgtattacg ccctggtcag tctcaatgcc
      841 ggcacggtgg ctgttattgt cgcatcagtt cctctattca cgcttgcgat tgccgttcta
      901 tttggtcagg agcgcctgtc attcaatcgg gtcgcaggtg gtatcctcgc gattgccggt
      961 atagcgattc tgcgcggctt tgggggcgat tttgttctct catactttgt tgcagctgta
     1021 gtcggcgcgg ttgccatcgc ggcttccagc gtggtcgcaa aggcataccc taatgtgcat
     1081 ccaatcaata tgaatgcaat tggcacagca gcaggaacgc tcctgctcgc cgtcagctcg
     1141 ctcgtatttg gcgagcaatg ggtgcttccg catgaaaaca cgacatggtt tgctgttggt
     1201 tggcttgtga ttgctggttc ggtgggtctt ttccagcttt ttctatttgt gatcaaacgc
     1261 tggactgcct cggcaaccgt atatgctctt acgggaatgc cagtgattgc cactgcactt
     1321 ggtattatca tactcgacca accggtcact atggaggttt tggcaggcgg cgcattggtg
     1381 attgcagcgg tctacgttgg cgccatcgca aggcagagga aaacatcgaa gttctaccgt
     1441 cagaaggctt agcgccatgc gaatcctgaa agcacagtaa gacccagatt aacacagctt
     1501 ttaacctgac caccttcggc gcgtacctag gtagctattc tctctgccgg ggtgatatac
     1561 tagcagatga ccaacccgcc ctccagcagg taatgcatac tattgagtgg tcttagggta
     1621 ttcctaagac caacgatact accaaaggaa cagcgctatg caaattatgc caactgaccc
     1681 tgtgattcgc gctgcgggtg g
//
LOCUS       DBNHOH010000214         1669 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_652928, whole genome shotgun sequence.
ACCESSION   DBNHOH010000214 DBNHOH010000000
VERSION     DBNHOH010000214.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_652928"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..1115
                     /gene="add"
                     /locus_tag="AC16V7_03810"
     CDS             111..1115
                     /gene="add"
                     /locus_tag="AC16V7_03810"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010635442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HIR6256276.1"
                     /translation="MASFIDPSFPWIDLHRHLDGSVRLETILDLGRKHNLPLPAWDVE
                     SLRPHVQITEPQPGVMAFIAKFKWMVGVLVDYAAVRRIAYENVEDAQKEGLDYVELRF
                     SPWFMAEPHHLRPAGVVEAVVDGVQAGIRDTGMRVKLIGILSRTYGPEIAWKELEALL
                     TQREQLVALDLAGDEANFQGELFNKHIRRGRDAGWNITVHAGEAAGPHSVWHAVRQMG
                     AARLGHAVHAAEDPSLLDYLAEQRIGIEANLTSNVQTSTVPDYPSHPLRRFLEHGILA
                     TINTDDPGISNITLAYEYDVAAPAAGLTPGQIRQAQRNALEVAFLTQEEKESLLAKGS
                     "
     gene            1118..>1669
                     /locus_tag="AC16V7_03815"
     CDS             1118..>1669
                     /locus_tag="AC16V7_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256277.1"
                     /translation="MHSWLKNTHVAYVPGPETSPVEEEFINSLLGQFRALGHYVQDVP
                     DPHTDILITTAPFGQPISWRKALLFTGRARYKYHHSPMVFTLIKVTPEVLDTQLAHFR
                     QALEKEEPDPKDYLFPGLASSAYKVLHEQARRGGAILALERLLQAQSKCINILLLVGD
                     QDIEAAYHFDLVGSHPLTIVRDNP"
BASE COUNT          385 a          485 c          449 g          350 t
ORIGIN      
        1 tcctcctcaa ttgtcaattt ttctgtttca ggatattata acaagaactt accccgctat
       61 tacatcaagt atcctgaaat aaagcatcac taaccgacct agcctgggcc atggcttcat
      121 ttatcgaccc ctctttccct tggattgatc tacaccgaca cctggatgga agcgtgcgcc
      181 tggaaaccat tctggacctg gggcgcaagc acaacctgcc cctacccgca tgggatgtcg
      241 aaagcctgcg cccccacgtg caaatcaccg aaccgcagcc cggtgtgatg gcttttatcg
      301 ccaagttcaa gtggatggtc ggggtgctcg tcgattacgc tgcagtgcgc cgcattgcat
      361 atgaaaatgt ggaagatgcc cagaaagagg ggctggatta tgttgagcta cgcttcagcc
      421 cctggtttat ggcagagccc caccacctgc ggcccgccgg tgtggtcgag gcggttgtgg
      481 atggagtcca ggctggtatt cgggacacgg gcatgcgggt taagctaatc ggcatcctca
      541 gccgcaccta tggcccggag atcgcctgga aagagctgga agccctgctc acccaacgcg
      601 agcagctggt agccctggac ctggccggcg atgaggcgaa ttttcaaggc gaattattca
      661 acaagcatat ccgccgcggg cgcgatgccg gctggaatat caccgtccat gccggagaag
      721 cggccgggcc gcatagcgtc tggcatgcag tgcgccagat gggcgcagcg cgcctgggtc
      781 acgccgtgca tgccgccgag gacccatcct tactggatta cctggcggaa caacggattg
      841 gaatcgaagc taacctgacc agcaatgttc agacgagcac tgtccccgac taccccagcc
      901 atcctctgcg ccgcttcctg gagcatggca tcctggcgac catcaacact gatgaccctg
      961 ggatcagcaa catcacactg gcatatgagt acgacgtggc tgcccctgcc gccggcctga
     1021 cccctggtca aattcgccag gcgcaacgca acgccctgga agtggcattc ctgacacagg
     1081 aggaaaaaga atcactgctt gcgaaaggtt cttaacgatg catagttggt tgaagaacac
     1141 tcacgtcgct tatgtcccag ggcctgagac ttctcccgtc gaagaggagt tcatcaactc
     1201 cctgctggga cagtttcgag cccttgggca ttatgtgcag gatgtgccag acccgcatac
     1261 agatatcctg atcacaaccg ctccattcgg tcagccaatc tcctggcgaa aagccttact
     1321 gtttaccgga cgggctcgtt ataagtacca tcactctccg atggttttca cgctgatcaa
     1381 agtcactccg gaagtgctag acacgcaact ggcgcacttt cgtcaggcat tggaaaagga
     1441 agagccagat ccaaaagatt atctctttcc aggtctggcc tccagcgcat acaaggtgct
     1501 ccacgagcaa gccaggagag gcggggcgat cttagcgctg gagagattgc ttcaggcgca
     1561 atcaaaatgc atcaacattc tattattagt gggcgatcag gacatcgagg cggcgtacca
     1621 ttttgatctg gtcggctcgc atccgctgac catcgtaagg gataaccca
//
LOCUS       DBNHOH010000215         3618 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1165946, whole genome shotgun sequence.
ACCESSION   DBNHOH010000215 DBNHOH010000000
VERSION     DBNHOH010000215.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1165946"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1867)
                     /locus_tag="AC16V7_03820"
     CDS             complement(<1..1867)
                     /locus_tag="AC16V7_03820"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6256278.1"
                     /translation="MFRKSIISQLSLVMIAVLTVLAPLSTGYAHPSDGTSIYTLDGAS
                     SESESIESFVDQYVADGMQAAHVPGFAVVVVKDGKTLISKGYGYADLEQRIPMTPQTN
                     LRAGSVSKPVVATAVMQLVDQGLVELDAPISRYIPDLELTDRFGQASTVAQLLTHQSG
                     YTDADLLSHAPTLEEWQPLGEYLHDNLGPRDIQPGVMAYSSRNTALLGYMIEKITGLP
                     YDQAIARSLFDPLGMSKTTFTQPLPDEIRANLATGYFYQGGTYEIVPLDYVRSSPGIA
                     MVTTAEDMGQLMQALLNGGQFNGMQVLKAETTETLLTRQAAAHTYSRGKSYAFSENRL
                     GGRQVLYHDGNGIGFGSRMILVPEHDLGIFISTNHRQVQFDANTHTPAFEFIRDLASA
                     ILEREVPETPQNISALTPLPDAAGRAGRYAGHYQLAESPLKDFFKIRLLLDNVDVSDN
                     GDGSITIGSKSYVEVEPLVFQRQDNASSFVVFIENARGEVEYLTFGGTGSYKKAGWYQ
                     TLNFTIGLVAGMLLIFLSYIVTWPFLRQGSWLAWAISLLNLIFLVGFGIMIATADIVL
                     FFKMIPLPMRLLMALPWISALGTLGLLVSQFRRSSRQGTSRWGQIHTLLMTIASLA"
     gene            2079..3275
                     /locus_tag="AC16V7_03825"
     CDS             2079..3275
                     /locus_tag="AC16V7_03825"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6256279.1"
                     /translation="MTTKLGYLTHRRPRALIFSLVSYLLWVTVTIRWITEFIEASHPL
                     TWLVSGMLVLYGILMGLEPLITKGSTWRAHLYLALQTALVMVAMLFYFELDFFAILLL
                     PLCGQAVLIFPRRAASIWVIIFVLANIVGQIHQFGWPQAISFILLYFGAMFFVAAFSF
                     LTVQAEEARQKSEELLGELREAHKKLQEYAGQTEELAVAKERNRLARELHDSVAQTLY
                     GLTLQSEAAARKLAAGQTQAAEDYMRDIRQSALNTLQETRLLIFELRPPILENEGLAA
                     ALRSRLEAVEARSGLETLIQVADIGRLPPHIEVGLYRIAQEALNNALKHAQANQISVS
                     LSQEGGKAILEVTDNGLGFDLSVVGTQGGMGLRGMRERAEQLGAKLSVESAPKAGTKV
                     KVEASL"
     gene            3272..>3618
                     /locus_tag="AC16V7_03830"
     CDS             3272..>3618
                     /locus_tag="AC16V7_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6256280.1"
                     /translation="MNSMSRVFIAEDHPIVRRGIRGLLETEPGIEVIGEASNGQDAVS
                     EVKRLKPDIVLMDLLMPIMDGIEATRQIRTNQPQVRILVLTSFATDDKVFPAIKAGAL
                     GYLIKDTPPEDLVR"
BASE COUNT          869 a          970 c          969 g          810 t
ORIGIN      
        1 aggccaggga ggcgatcgtc atgagcaagg tgtggatctg gccccaccgg gaagtgcctt
       61 gccgactcga gcgcctaaac tggcttacaa gcaatccaag cgtgcccagg gcactaatcc
      121 agggcagcgc catcaataag cgcatgggca gtgggatcat tttgaagaac aggacgatat
      181 ctgcggttgc gatcatgatc ccaaacccga ccagaaaaat caggttgagc aggcttattg
      241 cccaggccag ccaggagcct tgacgcagga aaggccaagt cacgatgtaa gacagaaaga
      301 tgagcagcat gccagccacc aacccgatgg tgaagttcag cgtctggtac cagcctgcct
      361 tcttgtacga tccggtgcca ccaaaggtca gatattccac ctcacctcgg gcgttttcga
      421 tgaaaacgac gaaggagctg gcattatctt gtctctggaa gaccagcggc tccacttcga
      481 cataactctt tgagccgatg gttatcgacc catcgccatt atcgctcaca tccacattgt
      541 ccagcagtaa tcggattttg aagaaatctt tcaacgggga ctctgccagc tggtaatgcc
      601 cagcataacg acccgctcgc ccggctgcgt ctggcaatgg tgtgagcgcg gagatatttt
      661 gaggcgtttc cggaacctcc cgctctaaaa tcgcgctggc cagatcccgg atgaattcga
      721 aagccggcgt atgggtattg gcatcaaatt gaacttggcg atgattggtg ctgataaaga
      781 tacccaaatc gtgctccggt accaggatca tccggctacc aaacccgatc ccgttgccat
      841 catgatacaa cacctgccgc ccaccgagac ggttttcgga aaatgcataa gactttccac
      901 gagaataagt atgagctgca gcctggcgtg tcagcagcgt ttccgtggtc tccgccttca
      961 agacctgcat gccattgaat tgaccaccgt tgagcagagc ttgcataagc tgccccatgt
     1021 cttcagcggt ggtcaccata gcgatgcccg gcgaggaacg tacataatcc aggggcacaa
     1081 tctcgtaagt gccaccctga tagaaatagc ctgttgccag attggctctg atctcatcag
     1141 gtagtggctg tgtaaatgtc gtcttgctca tgcccaaggg atcgaaaagg ctgcgcgcaa
     1201 tggcctggtc atagggaaga ccggtgattt tttcaatcat gtagcccaac agagcagtgt
     1261 ttcgactgct gtaagccatc acaccaggct ggatatcgcg cggacccaga ttgtcgtgta
     1321 gatactcgcc taacggctgc cattcttcga gggtcggggc atgcgagagc aagtcagcat
     1381 ccgtgtaacc gctttgatgt gtgagcaact gcgctaccgt gctagcttgt ccaaatcgat
     1441 cagtcagttc caggtccggg atgtatctac tgatgggggc atccaactcc accaagcctt
     1501 gatcgaccag ttgcatgaca gccgtggcaa cgactggctt tgacaccgat ccggcgcgca
     1561 ggttcgtttg cggggtcatc gggattcgtt gctcaagatc ggcatagccg taacccttgg
     1621 aaatcagggt tttcccatct ttcaccacga caaccgcaaa acccggaacg tgggctgcct
     1681 gcatgccatc ggctacatac tggtctacaa agctctcgat agattctgat tcactggagg
     1741 ccccatccaa ggtgtagata ctagtgccat ccgaaggatg ggcgtaacca gttgagaggg
     1801 gagccagcac agtgagtaca gcaatcatga ccagggacaa ctgagagatt attgactttc
     1861 taaacattgc acacctcctt atatttggtt tgctgaaatc tttgatctca gtatgcccaa
     1921 gttcacctta gttgtcatcc gaaaaagcct ggaaatggac atccataaaa ctatttatct
     1981 cccagacccc catatccata attttatgta ttgatgcggg ggatatttgt tcatataatg
     2041 atttcaattg ggtttccttc ggaattggat gtgcacacat gacaacaaaa ttaggctacc
     2101 ttactcatcg ccgaccacgc gcccttatat tctcgctcgt aagttacctg ctatgggtga
     2161 ccgtcacaat acgctggatt acggagttca tcgaagccag ccacccactg acctggctgg
     2221 tcagcggtat gctggtactc tatggcatcc tcatgggatt ggaacccttg attacgaagg
     2281 gctctacctg gcgcgcccat ctatacctgg ccttacaaac agccctggtg atggtcgcca
     2341 tgctctttta ttttgagcta gacttctttg cgatcctctt gttacccctc tgcgggcagg
     2401 cggtattaat atttccacgc cgggctgcgt ccatttgggt gattatcttc gtcttggcca
     2461 acattgtggg gcagatccat caatttggct ggcctcaagc tatttctttt atcctgctat
     2521 attttggagc tatgtttttc gtggctgcat tttcatttct cacagttcaa gctgaggagg
     2581 cgcgtcaaaa aagcgaagag ctattgggag aactccggga ggctcacaag aaactgcagg
     2641 aatatgccgg gcagacagaa gagctggcag tagccaaaga gcgcaaccgc ctggcacgcg
     2701 agctgcacga ttccgtcgcc cagaccttgt acgggctgac cctacaatct gaggctgctg
     2761 ccagaaaact ggccgctggc cagacacagg ccgccgaaga ctatatgaga gacatccgcc
     2821 agagcgcatt gaacaccttg caggagaccc gcctgctgat ctttgagctg cgccccccca
     2881 tactagagaa cgaaggcctg gctgctgctc tgcgctcccg gctggaggcg gtggaggcaa
     2941 ggagcgggct ggaaacgctc atccaggtgg cggatatcgg caggctgccc ccccatatcg
     3001 aagtgggcct gtaccgcatc gcacaagaag cgctgaataa cgccctcaag catgcccagg
     3061 ctaaccaaat cagcgtctcc ctctcgcagg aaggggggaa agccatcctg gaagtcaccg
     3121 ataacggtct cggctttgac ctctcggtcg tgggtaccca gggtggcatg ggcttgcgcg
     3181 gcatgcgcga gcgggctgag cagctaggag ccaaactctc ggttgaaagc gctccaaagg
     3241 caggaacaaa agtaaaggtg gaggcgtcat tatgaacagt atgagccggg tatttatcgc
     3301 cgaagaccac cctatcgtgc gccggggcat tcgtggccta ctagaaaccg agcccggcat
     3361 cgaggtcatt ggagaggcga gcaatggtca ggatgcagta agcgaggtta aaaggctgaa
     3421 gccggatatt gtgctgatgg atttattaat gcctatcatg gatggaatcg aggcgacgcg
     3481 ccagatccgt accaaccaac cgcaggtgcg catcctggtg ctgaccagtt ttgcaacgga
     3541 cgataaagta tttccggcga tcaaggcagg tgcgctgggc tacctgataa aagatacccc
     3601 gcccgaagac ctggtgcg
//
LOCUS       DBNHOH010000216         3660 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1743034, whole genome shotgun sequence.
ACCESSION   DBNHOH010000216 DBNHOH010000000
VERSION     DBNHOH010000216.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1743034"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="AC16V7_03835"
     CDS             <1..807
                     /locus_tag="AC16V7_03835"
                     /inference="COORDINATES: protein motif:HMM:NF046778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR6256281.1"
                     /translation="ADFDLVLFADGYQSLGRQLLFPEIGLDYRGYMLWRGLLPEREMD
                     DSAPLGSKLPRLSYSNLPGNLAIYFVPNHDGSVEEGNRIYNWAAYIPIPESELPEFMV
                     DRDGISHSGTIPPGNVRLEEENRLKRLMQDNLPDYYGEIIGKTENTYVQLIYTVRLPA
                     YHQGRLCLIGDAGMVAQPFTGSGVFKGYNNIKDMLQALNAYDRIDDALKHWGSEQVRI
                     GNRLLALGEQMEQAFIWNSLDLATADAKSTEAWWKQAVTFPEEFTYAADE"
     gene            1214..1600
                     /locus_tag="AC16V7_03840"
     CDS             1214..1600
                     /locus_tag="AC16V7_03840"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR6256282.1"
                     /translation="MHFIFPIQILVPIPLRYFGLGFITLGIILNYLSARQLKKMQTSI
                     QFDESPARLVRDGPFRVSRNPIYLGGVTVLLGMAIFLGSLITFIFPVILFLILDRIYI
                     PLEESRMEERFGVDYIEYKQAVRRWL"
     gene            complement(2152..2616)
                     /locus_tag="AC16V7_03845"
     CDS             complement(2152..2616)
                     /locus_tag="AC16V7_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256283.1"
                     /translation="MDKTRFLQIIRWGGAIALLLSACGMPIEIGGQPAVIGFSTDYEP
                     STSDSQTYTTNQDQSANEPTGVQEIQVDNVRVDLGVGSPVPVMVEISGTWPGPCSQLA
                     EIKQTAGEETFDIYLLATPDGPDCPLDQQGLFLEINIPLIWLGSRRARTGST"
     gene            complement(2693..>3660)
                     /locus_tag="AC16V7_03850"
     CDS             complement(2693..>3660)
                     /locus_tag="AC16V7_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256284.1"
                     /translation="RLLSDGIIRAAKMAYPESEGGFNFTFGDSITDPHTFRYDGIAWN
                     ADVILTRLFFLLVALVIVMLAAAFFDRFNPSKILIIKRKDGKLKSLEPAVTETIPLSQ
                     VHLTPSAGLPARFRFGALFIAELKLFLKGHRWWWYVITLGLVIAQLATDLKTARILLA
                     VTWLWPILLLSGLGSREARHNTREIVFSAPRPVLNQLPATWLAAFSVIALMGSGAFLR
                     FLFAGEITSLLAWLAGALFIPSLALTFGVLTGSSKAFEVVYVLWMYLILQEVPPLDFV
                     GVVPESPWAVYALLALGLFTLTAFVRQWQLKGGSVAKLAGKRRSI"
BASE COUNT          936 a          882 c          909 g          933 t
ORIGIN      
        1 gcagatttcg atctcgtatt attcgccgat ggttatcaat cgctgggtcg tcagttgctt
       61 tttcccgaga ttggcctgga ttatcgaggc tatatgttat ggcgtggttt gttgcccgaa
      121 cgggaaatgg atgacagtgc ccccttgggg agcaagttgc cacggctatc gtattcaaat
      181 ctacctggta atcttgcgat ttactttgtc cccaatcacg atggctccgt agaagaaggc
      241 aatagaatat ataactgggc ggcatatatc cccatcccag aatcagaact acccgagttt
      301 atggttgacc gtgacggaat ttctcactca ggtacgatcc ctcccggaaa tgtgagactg
      361 gaagaggaaa atcgcctaaa gcggctgatg caagacaatc tacccgacta ctacggtgag
      421 attattggca agaccgagaa tacctatgtg cagcttattt ataccgttcg cttacccgct
      481 tatcaccagg gccggttgtg tttaattggt gatgcaggca tggttgctca gccttttact
      541 ggctcaggcg tttttaaggg atataacaac atcaaagata tgcttcaggc gctcaacgcc
      601 tacgatagga ttgatgatgc tctaaaacac tgggggagtg aacaggtgcg gattgggaac
      661 cgcctgctgg ctttgggcga gcaaatggaa caggctttca tttggaattc gcttgacctt
      721 gccacagccg atgcgaaatc gacagaagcc tggtggaaac aagcggttac ttttccagaa
      781 gagtttacct atgctgccga tgaatagcag attgcgatta gtaggttaag ctgtgggggt
      841 gcccactata caaagttatg atggatagat ctgccagaag agacaaacgg ggtggtctat
      901 ttcttcaaag agggcggtgg cggtttccat agtggaatac gcctttaccc gacaaagggc
      961 atagcctctg tggtgatggt gaacagcacc gagttcgatt caaccaattt cttgtatcgc
     1021 gtggaaggag cgtttttcga tccatacaaa tttaaaatat tgcgcggcct aacaccgacc
     1081 agcagggcat acttctcctt gcgctttgca tagcgggtgg accgattgag acagtattct
     1141 ttccaaagac ataatgaaag tcaatccaat caatccatct tccttcaacc tcttaatacc
     1201 cctttcgatt gcgatgcatt ttatcttccc gattcaaatc ctcgttccaa ttccgttaag
     1261 atatttcggg ctggggttca tcaccttggg cataatcctt aattaccttt ctgcacgtca
     1321 gttaaagaag atgcaaacgt ccattcaatt cgacgagtct cccgctcgat tggtgaggga
     1381 tggtcccttc cgggttagcc ggaatccaat ctatttgggg ggcgtcaccg tgctattagg
     1441 catggctatt tttctcggct ctctaatcac ttttatattt cctgtgattt tatttctcat
     1501 cctggatagg atttacatcc cactcgaaga gagccggatg gaagagaggt ttggggtcga
     1561 ttacattgaa tataaacaag ctgtgaggag gtggctctga tcggtcgcaa gcataatcta
     1621 tcaaaaactt ttagctaacc cgcgttaagc caggctggct cgtcgacagt aacgaatttt
     1681 gcgagcccaa caaatctaag ggaagacacc agcaatatca gggcaatata tacaaatagt
     1741 tcaatctgcc tcttggctgg cttgaccgcc atggaggttg atttttcgtg tgagctcatt
     1801 atgcttcttt ccatgtgcat gcaaaatcga taaatttcct agccagaaat aattcagtga
     1861 attaaaaaaa agcctacttg agctttttat tataacctcg tcatgtaggt gatggatttc
     1921 aaacagcata aaagaataat gagtgcggag gagctcgaac gctcaaccga tggcttttct
     1981 ttacacttgc ttgcttcgat tgttattgaa tctgtaacat gagcgatgga ttttgataca
     2041 acaagcaagc tccaagaaag ctagtagctc tcacctgcta tctctaggag cctgccatgc
     2101 cgatctactt tgacaagctc ccgctccaat cgaagcttgt ttctttgcca ttcacgttga
     2161 ccctgtacgt gcccgtctgc ttcccagcca tattaaggga atattgattt ccaaaaataa
     2221 cccctgttgg tcgagagggc aatctggtcc atcgggtgtg gctaagaggt aaatatcaaa
     2281 ggtctcttcg ccggctgtct gtttgatttc ggccagctgg ctgcacggcc ccggccaggt
     2341 gccgctgatt tcgaccatca ccgggacggg tgaaccgacc ccaagatcaa cccgtacatt
     2401 gtctacctgg atttcctgca caccggtcgg ctcatttgct gattggtctt gattggttgt
     2461 gtaggtctga gaatcgctgg tcgaaggttc gtaatcagta gaaaacccga taacagctgg
     2521 ctgcccccca atttcaattg gcatcccaca ggcgctgagg agaagcgcta tagcccctcc
     2581 ccagcgtatg atttgtagaa atctcgtttt gtccatcgtc ttttccctta cctatataaa
     2641 catccaatct ttttagtgct ggcaggttgg aatggaagag gtaagctgcc tttcaaattg
     2701 atcttctttt gcctgccagc tttgctacgc ttccgccttt caactgccac tggcggacaa
     2761 atgccgtcaa ggtgaacaat cccagcgcca gcaaggcgta aacagcccag gggctttcgg
     2821 gaaccacccc cacgaaatcg agcgggggga cctcttgaag gatgaggtac atccacagga
     2881 cgtaaacaac ttcaaatgct ttgctgctgc cggtcaggac tccaaaagtc agcgccaacg
     2941 aagggatgaa cagcgcgcca gccaaccatg ctagcaagct ggtgatttct cctgcgaaca
     3001 ggaagcgtaa aaatgcgcca gaacccatca gcgcaatgac gctaaaggcg gccagccacg
     3061 tggcaggcag ctgattcagc accgggcgtg gggcagaaaa gacgatctcg cgggtgttgt
     3121 gccgcgcttc gcggcttccc aacccactga ggagcaaaat cggccatagc catgtgacgg
     3181 cgagcaggat gcgggcggtt ttgaggtcag tggctaattg ggcgataacc aaacccaagg
     3241 tgataacata ccaccaccag cgatgcccct tcaggaacaa tttcaactcg gcgataaaaa
     3301 gcgcgccaaa acggaagcgg gcaggtaagc cggctgacgg ggtgagatga acctgtgaaa
     3361 ggggaatcgt ttcggtgaca gcaggctcca gggacttcaa tttgccgtcc ttccttttga
     3421 ttattaagat ctttgagggg ttgaagcgat caaaaaaggc ggcggcgagc atcacgataa
     3481 ccaacgcaac tagcaggaaa aacaatcgag tcaggatcac atctgcattc caggcaatac
     3541 catcgtagcg aaaagtatgg ggatcggtga tgctgtctcc aaaagtgaaa ttaaacccgc
     3601 cttcgctttc aggataggcc atctttgcgg cgcggattat gccgtcactc agcagccgta
//
LOCUS       DBNHOH010000217         1350 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1358364, whole genome shotgun sequence.
ACCESSION   DBNHOH010000217 DBNHOH010000000
VERSION     DBNHOH010000217.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1358364"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="AC16V7_03855"
     CDS             <1..801
                     /locus_tag="AC16V7_03855"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR6256285.1"
                     /translation="ASRVFYPRNGSQWVEVNHIPLRLPRLSPKFHGYRLVQISDFHLG
                     TWLNYNDLRHAVELVNDQNPDLVVITGDFVTINSQKYYSDLVAALGILHATDGVVAVL
                     GNHDHWSDSAEIIRTLNQINIKILCNDVFTLKRGKEQLHICGIDDMLVGRDHLDQVLL
                     MLPEGGAAILLAHEPDFADISAATRRFDLQLSGHSHGGQIVLPWIGIPFLPSYASKYV
                     SGLYRVKDMVQYTNRGLGTSELQIRVNCPPEISVFTLEAVKLPKHDSS"
     gene            847..>1350
                     /locus_tag="AC16V7_03860"
     CDS             847..>1350
                     /locus_tag="AC16V7_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR6256286.1"
                     /translation="MREVAIIGIGQTPVGEHWEKSLRELAGEAIVAAMQDANRERVDG
                     LFIGNMLSGMVNHQENLGSLIADWVGLQGIEAFKVEAACGSGAAALRVAIMAVASGEM
                     ESAIALGVEKMTDNTGTDTTAALATAADADYEAAMGLSFVAINALIMQRYMFEFGWKH
                     DDFAPFSI"
BASE COUNT          362 a          306 c          337 g          345 t
ORIGIN      
        1 gcaagccggg tattttaccc acgaaatggc tcacaatggg ttgaggtcaa tcacattccg
       61 ctacgccttc cgcgcctctc gccaaagttt cacggttatc gcctggttca gatcagcgat
      121 tttcacctgg gcacttggtt aaactataat gatctgaggc acgctgttga gcttgtgaac
      181 gatcagaatc cggacttggt cgtaataact ggtgatttcg taaccataaa ttcccaaaaa
      241 tattactcag acctggttgc tgcactcggc attcttcatg caactgatgg agtagtggca
      301 gtgcttggta atcatgacca ctggagcgac tcagctgaaa taatccgcac acttaatcag
      361 atcaacatta aaatcttatg taacgatgta tttacactta aaagaggaaa agaacagctc
      421 cacatatgtg gaatcgacga tatgctggtg gggcgcgatc acctagatca ggtgcttttg
      481 atgcttccag aaggcggagc tgcaatcttg ctagcccatg agccagactt tgccgatatc
      541 agcgcagcaa cacgccgatt tgatctccag ctttcaggac attctcacgg tggtcaaatt
      601 gtcttgccat ggattggcat acccttcctg cccagttatg caagtaagta tgtctctggt
      661 ttgtatcgcg tgaaagacat ggttcagtat actaatagag gattgggcac atccgaactt
      721 cagataagag ttaactgtcc tcctgaaatt tccgtattca cattagaggc ggtaaaatta
      781 cccaagcatg atagctcttg ataggataaa cacctacaat attcaatgga aaaggattgt
      841 agctgaatgc gcgaggtagc tatcattggt atcggtcaaa ccccagtcgg tgagcactgg
      901 gaaaaatccc tgcgcgagct agcaggggag gcaatagtcg cggctatgca ggacgccaac
      961 agggagaggg ttgatgggct gttcattgga aatatgctct ctggaatggt gaatcatcag
     1021 gaaaacctgg gatctcttat tgccgattgg gtaggattgc aagggataga agccttcaaa
     1081 gtggaagccg cctgcggttc aggcgcagcc gcactgcgcg tcgctataat ggcagtggcc
     1141 tctggagaaa tggaaagcgc cattgccttg ggcgtggaga agatgactga taatacaggc
     1201 acagacacta ctgctgcact cgccacggct gccgacgcag attatgaagc tgcaatggga
     1261 ttatcatttg ttgccatcaa tgccttaatc atgcaacgat acatgtttga atttggttgg
     1321 aaacacgacg atttcgcccc cttttctatc
//
LOCUS       DBNHOH010000218         1476 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_268076, whole genome shotgun sequence.
ACCESSION   DBNHOH010000218 DBNHOH010000000
VERSION     DBNHOH010000218.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_268076"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..528)
                     /locus_tag="AC16V7_03865"
     CDS             complement(79..528)
                     /locus_tag="AC16V7_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256287.1"
                     /translation="MWNRIARQVQSYSSAVLTFVEGNGYPFSVRCDPQYDRSQQVFRL
                     HIPNQIALQSGPAGLLCHQHDKNLWNLKSMVLRGILEEDDKGWFFRPLQFIPGMGGFF
                     GYVRFVAQGRRLARNYLERRGLDRPTIPWAEYATIVKLAKKDKFQIE"
     gene            complement(530..1063)
                     /locus_tag="AC16V7_03870"
     CDS             complement(530..1063)
                     /locus_tag="AC16V7_03870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6256288.1"
                     /translation="MGDITHEFTLPPDVEAVFKKFWTCEFTTLAKDGTPVTWPILPIY
                     QPGLRSFVFLTSIGTPQKVFNIRRNRRVSLFYSDPTGSDLVDPPAVLVQGDAQVGDQI
                     ITSREQIDEQLSLDMQEQGRRLLKHQPAITMYTNNRLTQYVMDWYFMRLLITITPRRI
                     LWWEHGDFTSPAHVIEV"
     gene            complement(1222..>1476)
                     /locus_tag="AC16V7_03875"
     CDS             complement(1222..>1476)
                     /locus_tag="AC16V7_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256289.1"
                     /translation="LPWPLYALSLWLFGFVLLAAWKQGSPAGVALLLLAAGGHAPQLS
                     TQLFFGLVGLSLLTTRLPSSDFATYPAQAASASPPALEIG"
BASE COUNT          407 a          389 c          339 g          341 t
ORIGIN      
        1 gggcatcgcc cgcccaaacc cccctgccac gtatccaact acgtaacttc taaattaatt
       61 atactcgggt aagccctctt actctatctg gaatttgtcc ttcttggcta gtttcactat
      121 ggtggcatat tcagcccaag gtatggtagg gcgatccagg ccacgacgct ccaggtaatt
      181 gcgagcaaga cgccgtcctt gggcgacaaa tcgcacataa ccgaagaatc cacccatccc
      241 cggaataaat tgcaacggtc gaaaaaacca acctttatca tcctcttcca gtatgccgcg
      301 cagcaccata cttttcagat tccacaggtt cttgtcatgc tggtggcaaa gcaggccggc
      361 tgggccagat tggagggcaa tctgatttgg tatatgcagg cgaaacactt gctggctgcg
      421 atcatattgc gggtcacagc gcacgctaaa agggtaaccg ttcccctcga caaaagtcaa
      481 taccgcgctg gaataagatt gaacctgcct agcgatgcga ttccacaccc tacacctcaa
      541 ttacatgtgc tggagaagta aaatctccgt gttcccacca taaaatgcgt cgcggtgtga
      601 tcgtaatcag cagacgcatg aaataccaat ccatcacata ctgggtaagg cgattgttgg
      661 tgtacatagt tatcgccggt tggtgcttca aaagtctgcg cccttgctct tgcatatcta
      721 aacttaactg ttcgtctatc tgctccctgg aggtgatgat ctgatcccca acctgagcat
      781 ccccctgcac gagcacagct ggcggatcga caagatcact ccccgttgga tcggaataaa
      841 acaacgagac gcgccggttt cgccgaatat taaaaacctt ttgtggtgta ccgatagagg
      901 tcaggaaaac aaaactgcgc agcccgggtt gataaatggg caagatgggc caagtgacgg
      961 gcgtgccatc tttggcaagc gtggtgaact cacaagtcca gaacttttta aaaaccgctt
     1021 ccacatcagg tggaagtgta aattcatggg tgatatcacc catcgttcat cctcccgcaa
     1081 aaatcctgcc attaccccac ctattataag tacacagcta ttctttcaat tgttatacag
     1141 gtggttatta atgggaaagt acaaacggct ttcgccaagc ataatcccct cttctgtaat
     1201 tcaacgttgc tgtactaaat tttaacctat ttcgagtgca gggggagaag cggaagccgc
     1261 ttgagcggga taagtagcaa aatctgatga gggtagtctg gttgtaagca gactcagccc
     1321 gaccaaaccg aaaaacaact gcgtgctaag ctgaggagca tgtcctccag ctgccagcag
     1381 aagcaaggca acacctgcgg ggctaccttg cttccatgca gccaataaaa cgaatccaaa
     1441 cagccacaag ctaagcgcat acaaaggcca gggtaa
//
LOCUS       DBNHOH010000219         8701 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1166274, whole genome shotgun sequence.
ACCESSION   DBNHOH010000219 DBNHOH010000000
VERSION     DBNHOH010000219.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8701)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8701)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8701
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1166274"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1390
                     /locus_tag="AC16V7_03880"
     CDS             <1..1390
                     /locus_tag="AC16V7_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256290.1"
                     /translation="ALLSLGATPLQTGFHFIQIILQDSIHNLLGAWYQTINPEGLDLS
                     DRSVLASLALAVLTAGLALLFLLRVKGEEGDRPSYETSARWVRQAIWIGLLGAFLGPI
                     PIWVIDRQSLFGLQSGRFALAAMTGLSIFMIGVLEWFTPRRLPKSVLLAVLIGMAAGS
                     HLRVATSYYRSTLLQNQFYWQLYWRAPYIKPGTAILSKDELFIYVGRKPTAVTLNLLY
                     PQPFGTRNVGYWFLEMDHDIGAKMVPKLARGKTFTESFRTFDFTGSSLDSLVIFYKPG
                     AGRCLWVLSPEDADNPELPDLTLQALPVSNLSRIQAQPISNDYPPTEFFGEELEHNWC
                     YYYQKAELAAQFGAWQQVAELGDEALAKGFSPGNPHEWLPFIEGYARSRRWENAIART
                     QAAFTANELIAPRLCSLWKRILAQDVPPADFKSSIQQMQNLLQCNQAPNENSLSIKYS
                     STSIVVFDRTYP"
     gene            1677..2858
                     /locus_tag="AC16V7_03885"
     CDS             1677..2858
                     /locus_tag="AC16V7_03885"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008443 - phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR6256291.1"
                     /translation="MSKRVAILTGGGDVPGLNMCLKSLVYSLIDSGFEPIGVRKGWDG
                     FIQYNPHDPSSYSESFIELTKNMVRPIDRTPGSFLHSSRLDPRSVPRPLVPQFLRKSK
                     EEIQDLTNHIKDVVQRLGYQCLITIGDDDMLHYAAHLSEQGVPVIAIPKTIHNNIHGT
                     DYTLGFSTGMARGVSFMHELRALAGSREQVFVVETFAAKSGYSTLLMSFLGGVDRVII
                     PEVPYDPELLAQLVILDKKQTPANYALVLVCDGSSVREDKIEKFASVLPTGDGTEVMR
                     AHLSGDQKLVGSGMVAAQLLRHLLGEETFLQQLTYLLRTGPPDGQDLLGAANFAIIAS
                     RLAQEGRYGRMAAFVQDQMWTDVDLNLAIEGVRTVDVDAWYDTKEYRPKLSIIWSAGE
                     V"
     gene            2887..3615
                     /locus_tag="AC16V7_03890"
     CDS             2887..3615
                     /locus_tag="AC16V7_03890"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020485422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6256292.1"
                     /translation="MSSANGNNRLVQIVDLVKSFPVGGGEVTILKGLSFDVKQGEFVS
                     IVGPSGNGKSTLLNMITGIDRPTAGQVIVTGLEINKMSENQLAAWRGKHVGIIFQFFQ
                     MLPALSLLQNVILPMDFAKKYSPRERRERGMHLLEIVGLEDQAHKLPSMVSGGQQQRA
                     AIARSLANDPDLLVADEPTGNLDTRNAGDVFDLFLKLVDQGKTLLMVTHDKELSRRVP
                     RVVEIIDGKITRDEFVGGAVWTGY"
     gene            3627..6023
                     /locus_tag="AC16V7_03895"
     CDS             3627..6023
                     /locus_tag="AC16V7_03895"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6256293.1"
                     /translation="MGVLRYKIYRDLWVNFGRTLQVVLIIAIGAGAIGMIVGTRYLVI
                     SNMQSAWRAMNPATINLFIGPPIDEDDLIALESEDGVVEIEGMGDSNIEWRLNPNDEW
                     QLGGLTFRANYEEQKLNMLELVEGDWPSGKFVSIGQGDDQFFGVPKNGKVYLRLDEKE
                     YTIQTDGVVYNQLQQPAYFGGTAQFYATQEEYERIVGDRDFSQVLVTGVEYDELKLAE
                     LGDRLQDRIEKMGSDSSRMILDPNEHFFQPQLDGIFLILGVMGGLALALGLLLVYTTI
                     NAIITQQVDQIGIMKAIGARTWQILRLYLTSIFFYSILSLLIALPLGLGGAWSITSWL
                     VTGFGANPGSFDFSREAVIVMLLIVFLAPLLASLLPIFNGARITVREAISTYGLSTGA
                     GLLERLLAKAKAMSRLLLMVISNTFRNKGRVVLMQITLVLSGLIFMMVVSLRDSVVYS
                     VKDILFSILNANITFLFEEPQRIHYLEDLTMKYPGVKAVEMWGFAGVNMRPAGQEASE
                     DDELASVWGVPLPTQTYGYQLRSGRWLEPTDTHAIVMNQQLADDVGVTVGDWITVKYD
                     EKNEVNWQVVGLVFDPILTNVALASREVLLHDLRFVGRAQSAWIQTERDDMQTEIAVA
                     KGLREFYKQNGVEVSLRKGVFGMGGDSTTQAANALIAQFNTFIALPGLLAVIIAAVGG
                     IALSGVLTLSVLERRREIGVMRAIGASSFTIARLFIGEGLLLGWMSWLIALPLSIPVG
                     RLMPQAVGSALQLDLVYKYTPTGAVLWLVIITFLSIIASWLPARGATRISVRESLAYQ
                     "
     gene            6038..7222
                     /locus_tag="AC16V7_03900"
     CDS             6038..7222
                     /locus_tag="AC16V7_03900"
                     /inference="COORDINATES: protein motif:HMM:NF052757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR6256294.1"
                     /translation="MKSKLFTLIIIIALGSLFLSACGSIGAPADDAAPTEIPIVITDT
                     DVVAEGRLVPNDSANLSFKAAGQVTEILITEGDTVEAGQVIARLDNSEQLQSAIANAE
                     AELLNAQLARDALFDNAKVNSAAALQAIADARDAVRDSDRYLNNLNAGSRDTDIDLAK
                     ADVAILKDQLDEAQEDYKPYENKPEDDLKRATFLARFAQAEQLYKDAVRKLNNLQGEP
                     SKIDLDIAEANQAVAEAQLLLALQNYDEIASGPDPDDLAAVEARIKAAEAGLVSAQAT
                     LNNIELVAPFDGKIVDLVITTGEQVAPGVTAAVLADFSQWIVETDDLTEIEVPEISIG
                     QSVTIVPDALPDLELTGMVERISDVFEEKRGDITYTARIKLNEENELLRWGMTVVVTF
                     EK"
     gene            7359..8576
                     /locus_tag="AC16V7_03905"
     CDS             7359..8576
                     /locus_tag="AC16V7_03905"
                     /inference="COORDINATES: protein motif:HMM:NF052757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR6256295.1"
                     /translation="MTGVSLLLIFGSILLPACSAVGIGPSSTPTPTVTPEVESVIQVS
                     AEGQVVPRHHANLSTAVSGQVTEILFREGEQVQEGDIILRLGDREQYQADQAAAELEL
                     LSAEQALEELQKNVGVELAQTRQALALANQERSKIFDEYQNLSKPVPQDVLDQAYASV
                     LMAEKRLQKAGEDLEKMEKRYKNKSNIMWQFLNKKQFKDLLEQMRRARLSAEDRYYDV
                     LDRYNDLKNHPDEIELAKATANLGLVDARITDLQGDIAMLEKGPDPDKVASAEARRKA
                     AQVALEAAQLALNNSEIEAPFSGQIAEMNVTQGEWVEANQPVILLADLNHWEVQTDDL
                     TELDVPIVSLGQTVTIKLDALPELELKGKVDAIKDLSEEKRGDVTYTVTILLDEVDPR
                     LRWGMTAAVEFSD"
BASE COUNT         2051 a         2356 c         2380 g         1914 t
ORIGIN      
        1 cgcactcctt tccctgggcg ctaccccctt acagaccggg tttcatttta tccagatcat
       61 cctgcaagac tcgatccaca acctgttggg tgcatggtac cagaccatca acccagaggg
      121 tctggattta agcgatcgat ccgtactcgc ctcgctggca ttggctgtac tgactgccgg
      181 actggcgctc ctgtttctcc tgcgcgtgaa gggcgaggaa ggtgatcgac catcatacga
      241 aaccagcgcg cgatgggtgc gacaagccat ctggatcgga ctgctgggtg cttttttggg
      301 gcctatccca atttgggtta tcgaccggca gtcactcttc ggcttgcaga gcgggcgctt
      361 cgcactggcg gcaatgaccg gtttaagtat cttcatgatt ggcgtgctag agtggttcac
      421 cccgcgccgg ctgcctaaat cggttttact ggcggtgttg attggcatgg cagccggatc
      481 ccacctgcgc gtggccacct cttattaccg ctccaccctg ctacaaaatc aattctattg
      541 gcaactctat tggcgcgccc cctacatcaa acccggcacg gctatcctat ccaaggatga
      601 gctgttcatc tatgtaggcc gcaaacccac tgcagttacc ctaaacctgc tttacccgca
      661 gcccttcggt acgcggaacg tgggctactg gttcttggag atggatcacg atatcggtgc
      721 aaagatggtg cccaagctgg cgcgcggcaa gaccttcacc gaatcatttc gcacctttga
      781 ctttaccggc tccagcctgg acagcctggt gatcttctac aaaccaggag ctggccgctg
      841 cctgtgggtg ctctcaccag aagatgccga caatcccgag ctgcccgatc taaccttgca
      901 agccttgcct gtctccaatc tcagccgcat ccaagcccaa cccatctcca acgattaccc
      961 cccaaccgag ttctttggcg aggagcttga gcacaactgg tgttattact atcagaaggc
     1021 tgagctggct gctcagtttg gagcatggca acaggtggca gagcttggtg acgaggcact
     1081 cgccaaggga ttttcaccgg gcaacccgca cgaatggctg ccttttatcg aaggctatgc
     1141 acgcagcagg cgatgggaaa acgccatcgc gcggacgcag gctgccttca ctgccaatga
     1201 attgattgcc ccgcgccttt gtagtctgtg gaagcgtatt ttggctcaag atgtaccacc
     1261 ggcagacttt aaatcgagca tccagcaaat gcaaaatctg ttacaatgta atcaggctcc
     1321 caatgagaat agcctttcga tcaaatactc ctctactagc attgtggttt ttgatcgcac
     1381 ctatccataa acttcatcgt gaatttttaa catatctggc cataaccttc agcagattgg
     1441 cgatttaacg tcctcagctt ggctttggcg atgaaggtgc atattctcac acaattctgt
     1501 tactagcatt tgacctaaag ggtgggtttg aaatgagcca gataacaatt gtaaatctac
     1561 tactacatgc agccctgcgc gacccggctt aaataatctc gtatatagac aagattgagt
     1621 tgcattagaa atgagcaact ctcttgtcca agccacttta acataggaga atgaacatga
     1681 gcaagagagt tgccatactg accggtggcg gtgatgtccc tggcctgaac atgtgtctaa
     1741 aaagcttggt ttacagtctg attgattcgg gcttcgagcc cataggcgtg cggaagggat
     1801 gggatggatt tatacagtac aacccgcacg acccgtcctc ctatagcgag tcctttatcg
     1861 agttgaccaa gaacatggtt cgccccattg accgcacacc cggatccttc ttacattcat
     1921 cccggcttga tccccggtct gtgccgcgcc ccctggttcc acagtttctg cgtaaatcca
     1981 aagaagagat tcaggacctg acgaaccaca tcaaggatgt cgtgcagcgg ctgggttacc
     2041 agtgtctaat taccatcggg gatgacgaca tgctccatta cgctgcccat ctaagcgaac
     2101 aaggcgtgcc tgtgatcgct atccccaaaa ccatccataa caacattcac ggcaccgatt
     2161 acacacttgg cttcagcacc ggcatggcgc gtggcgtgtc tttcatgcac gagctgcgtg
     2221 ccctggcggg ctcgcgtgag caggtctttg tggtggagac cttcgccgcc aaatcgggct
     2281 acagcaccct gctcatgtca ttcctgggag gcgtcgaccg ggtgatcatc cccgaagtgc
     2341 cctacgaccc ggagctatta gcccagctcg tgatcctgga taaaaaacaa acgcctgcca
     2401 attacgccct ggtgttggtc tgcgatggct caagcgttag agaagacaaa atcgagaaat
     2461 tcgcttcggt gcttcccaca ggggatggga cggaagtaat gcgcgcccat ctatcgggcg
     2521 accagaaact ggtcggcagt ggtatggtag ccgcccagtt gctcagacac ctgttgggcg
     2581 aggaaacgtt tctccagcaa ctcacctatc tactccgtac cggtccacca gatgggcaag
     2641 acttgctcgg cgcggcgaat ttcgccatca tcgcctcccg cttggcccag gaaggacgct
     2701 atggtcgcat ggcggctttt gtccaggatc aaatgtggac cgacgttgat ctaaacctcg
     2761 ctattgaggg cgtgagaacc gtagatgtgg atgcctggta cgacacgaag gagtatcggc
     2821 caaaactgag tattatctgg tcagctggag aggtataagc aatcgacctg ggaacggaga
     2881 aagcatatga gtagcgcaaa tgggaacaac cgcttagtcc agatcgttga tctggtaaag
     2941 agcttcccgg tgggtggcgg ggaagtgact atcctcaaag ggttatcgtt cgatgttaaa
     3001 caaggggagt tcgtctccat cgttggtccc tccgggaacg gcaaatcaac gctgctcaat
     3061 atgatcaccg gtattgatcg acccaccgcc ggacaggtga tcgtcacggg actggaaatc
     3121 aacaagatga gcgagaatca gctggcagcc tggcgtggca agcacgtcgg tatcatcttc
     3181 cagttcttcc aaatgttgcc ggcgctcagc ctgctgcaaa acgtcatcct gccgatggat
     3241 ttcgccaaga agtacagccc tcgcgagcgg cgggagcgcg gcatgcacct gctggagatc
     3301 gtcgggttag aggaccaggc gcataagctg cctagcatgg tctccggcgg gcagcagcag
     3361 cgcgccgcca tcgcccgctc tctggcgaac gaccccgatc tactcgtagc tgacgagccc
     3421 accggcaacc tggacacgcg caacgccggc gacgtcttcg acctgtttct caagttggta
     3481 gatcagggta aaacccttct catggtgacg cacgataaag agctctcacg gcgcgtgcca
     3541 cgcgtggttg agatcattga tggtaagatc acccgcgacg aatttgtggg tggtgcagta
     3601 tggaccgggt attaaggatt tagctgatgg gcgtcttgcg ttataagatt taccgcgatc
     3661 tatgggtcaa tttcggccgt accctgcagg tggtattaat catcgccatc ggtgcaggtg
     3721 ctattggcat gatcgtgggc acgcgctatc tggtcatcag caacatgcaa tcagcctggc
     3781 gcgccatgaa cccagctacg atcaacctgt tcatcgggcc accaatagac gaggatgact
     3841 taatcgcttt agaaagcgaa gatggtgtgg tcgagatcga aggtatggga gattccaaca
     3901 tcgaatggcg tctcaatcca aacgatgaat ggcaactcgg agggctgacc ttccgtgcca
     3961 attacgagga gcagaagctc aacatgttgg aactcgtgga aggcgattgg cccagcggaa
     4021 aatttgtgtc catcgggcag ggagatgacc aattcttcgg cgtccccaag aatgggaaag
     4081 tctacctgcg cctggacgaa aaagaataca ccatccagac cgatggcgtc gtatataacc
     4141 agcttcagca gccggcgtat tttggcggca cggcccagtt ctacgccacc caggaagaat
     4201 atgagcgcat tgtcggcgac cgcgatttta gccaggtgct ggtgacgggc gtagaatacg
     4261 atgagctcaa gttggccgaa ctgggagatc gcctgcaaga tcgcatcgaa aagatgggca
     4321 gcgattcaag tcgcatgatt ctcgatccta acgagcactt cttccaacct cagctggacg
     4381 gtatattctt aatattgggc gtcatggggg gattggcatt agccctgggt ctcttactgg
     4441 tgtacaccac gatcaacgcc atcatcaccc agcaggtgga ccagatcggc atcatgaaag
     4501 ctatcggggc gcgcacctgg cagatcctgc gcttgtacct gaccagtatc ttcttttaca
     4561 gtatcctgtc gttgttgatt gccttgcccc ttggattggg tggtgcttgg tctatcacgt
     4621 cgtggttggt tacaggcttt ggcgccaatc cgggctcttt cgatttttca cgtgaagcag
     4681 taatcgtcat gctgttgatc gtattcctgg cgccgttatt ggcatccctg cttcccattt
     4741 tcaacggggc gcgcatcact gtgcgcgagg ccatcagcac ctacggtcta agcactggcg
     4801 ccggcttact cgaacgcctg ctggccaaag ccaaagccat gtcgcgcttg ttgctgatgg
     4861 tcatcagcaa cactttccgc aataaggggc gcgtggtgct gatgcagatc accctggtgc
     4921 tcagcggatt gatcttcatg atggtggtga gcttgcgcga ctcggtggtg tactcggtca
     4981 aggatatcct cttctctatc ctgaacgcca atatcacttt cctgtttgaa gagccgcagc
     5041 gtatccatta cctggaagat ttgaccatga aataccctgg cgtgaaggca gtcgaaatgt
     5101 ggggctttgc tggagtgaac atgcgcccgg caggtcagga agcatctgag gacgacgagc
     5161 tcgcttcggt atggggtgta cccttgccca cccaaaccta cgggtaccaa ttgcgctccg
     5221 gtcgctggct ggaacccacc gatacccatg ccatcgtcat gaaccagcaa ttggccgatg
     5281 atgtgggtgt gaccgtagga gattggatca ccgtcaaata tgatgagaag aacgaagtga
     5341 attggcaagt tgttggattg gtattcgacc cgatcttgac caacgtcgca cttgcttccc
     5401 gcgaagtgtt gctacacgac cttcgctttg tgggtagagc ccagtcagcc tggattcaaa
     5461 ccgagcgcga cgacatgcaa acggagatcg cggttgccaa aggtttgcga gaattctaca
     5521 aacagaatgg ggtagaggtc agcctgcgta agggtgtatt cggcatggga ggcgacagca
     5581 cgacccaggc cgccaacgcc ctgattgcac agtttaacac atttatcgca ctgccagggc
     5641 tcttagcggt catcattgcg gcggtgggag gcattgcatt gagcggcgtg ctcacgttga
     5701 gcgtgctgga acgccgccgg gagattggcg tgatgcgcgc catcggagct tcttcgttca
     5761 ccatcgcgcg cctgttcatc ggcgagggct tgctgctggg ttggatgagc tggctgatcg
     5821 ccctgcctct cagcatcccg gtggggcgcc tcatgccaca agccgtgggc agcgccctgc
     5881 agcttgattt agtatataaa tacacaccta cgggcgctgt gctctggctg gtcattatca
     5941 catttttgtc aattatcgcc agttggctgc ccgcgcgagg ggctacgcgc atcagcgtgc
     6001 gcgagagtct ggcttaccaa taaaggagac acctgggatg aaatctaaat tgttcactct
     6061 gatcatcata atcgctctgg gcagcctgtt cttgtctgcc tgcggctcga ttggcgcgcc
     6121 agccgatgac gccgccccga ccgaaatccc catcgtcatc accgacaccg atgtggtcgc
     6181 cgagggtcgc ctggtgccga atgattcggc caacctgtcc tttaaagcag ccgggcaggt
     6241 gaccgaaatt ctgatcaccg agggagacac cgtcgaagct gggcaggtta tcgctcgcct
     6301 ggataacagt gagcagctgc aatcggcaat tgccaacgca gaggcagagt tgctaaacgc
     6361 acagctagcc cgcgacgccc tgtttgacaa tgctaaggtg aactccgccg cggctctcca
     6421 ggctatcgcc gacgcccgcg acgcggtgcg cgactcagac cgatacctga acaacctgaa
     6481 tgctggcagc cgggacaccg acatcgatct ggccaaggca gatgttgcca tccttaaaga
     6541 ccagttagat gaagctcaag aagactataa gccatatgag aacaagcccg aggacgacct
     6601 caagcgagct accttcctgg cccgatttgc ccaagctgag caattatata aagacgcggt
     6661 ccgcaagctg aacaatcttc aaggggagcc cagcaaaatt gaccttgaca tcgcggaggc
     6721 caaccaggct gttgccgagg cgcaactact cctcgcctta caaaattatg atgaaatagc
     6781 ttctggtcca gatccagacg acctagccgc ggttgaggcg cgcattaaag ccgccgaggc
     6841 tggcttggta tcggcccagg caaccctaaa taatatcgag ctagttgccc cattcgatgg
     6901 caagattgtc gatctggtaa tcaccaccgg tgagcaagta gctccgggag tgacggctgc
     6961 cgtgctggca gatttctctc aatggattgt cgaaaccgat gacttgaccg agatcgaagt
     7021 ccctgagata agcataggcc agtccgttac aattgtcccc gacgccttgc cggacctgga
     7081 gctgaccggc atggtcgagc gcatcagcga cgtatttgaa gagaagcgcg gggatatcac
     7141 ctacacggcg cgcatcaagc tcaatgagga gaatgagctc ttgcgctggg gcatgaccgt
     7201 ggtggtcacc tttgagaaat agcgccatta accagcaatt tgtacgcata ggcttcttta
     7261 agcaattgga tcgaaatgtg tctgcagatg aattgtaggc aaacgaaaag caagaagaaa
     7321 aggacgctat gtctgtaact cgaaaaataa gtcccctaat gacaggtgtg agtctcctgc
     7381 ttatctttgg ctctatcctg ctacctgcct gcagcgcagt ggggattggg ccatccagca
     7441 ctcctacacc gacagtcact cccgaggttg agagcgttat ccaggttagc gccgaaggcc
     7501 aagtcgtgcc gcggcatcac gccaacctct ccaccgcggt aagcgggcag gtgaccgaga
     7561 ttctcttccg cgaaggcgag caggtccaag aaggtgacat aatcctgcgc ctgggcgacc
     7621 gcgagcaata tcaagccgac caggccgcgg ctgaactgga gctgctgagc gccgaacaag
     7681 cgctcgaaga gcttcaaaag aatgtcgggg tggagctggc ccaaacccgc caggcattag
     7741 ccctggcaaa ccaggagcgt tcaaaaatct ttgatgagta ccagaactta agtaagcctg
     7801 tcccccaaga tgtgcttgat caggcttatg ccagtgtcct catggctgaa aagcgattac
     7861 aaaaggctgg cgaagacctg gaaaaaatgg aaaaacgcta caagaacaag agcaacatca
     7921 tgtggcaatt cctgaacaag aagcagttca aggatctgct cgagcagatg cgtagagcca
     7981 ggctgtctgc agaggatcgc tattacgatg tactcgaccg ttacaacgat ctaaagaacc
     8041 acccagatga gatcgagctc gccaaggcta ctgcaaacct tgggcttgtg gatgcccgca
     8101 tcacagacct tcagggcgac atcgctatgc tagagaaagg tccagaccct gataaagtag
     8161 cctctgcaga agcccgccgc aaagcagctc aggtcgctct ggaggcggct cagctggctt
     8221 tgaataacag cgagattgaa gcacccttct ctggtcagat cgcggaaatg aacgtcacgc
     8281 aaggcgagtg ggtggaagcc aaccagccgg tgatcctgct ggcggacctt aatcactggg
     8341 aggtccagac cgatgacctg acagagctgg atgtgccaat tgtaagtctg gggcagacgg
     8401 tcaccattaa gctagatgcc ctgccagagc tggagttaaa aggcaaggtg gacgccatca
     8461 aagatctttc tgaagagaag cgcggggatg taacatacac ggtgactatc ttgttggatg
     8521 aggtggaccc gcgcctgcgt tggggcatga ctgccgcggt cgaattcagc gattaagcca
     8581 aacattggcg aatatttcaa aacatggaca ataaaccaac aagcccctta gcggggcttg
     8641 ttttcttcat cgaagctcac aaaatagaaa acccggctaa tctctgaaaa ctatctcggg
     8701 a
//
LOCUS       DBNHOH010000220         1879 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_589090, whole genome shotgun sequence.
ACCESSION   DBNHOH010000220 DBNHOH010000000
VERSION     DBNHOH010000220.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_589090"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="AC16V7_03910"
     CDS             <1..801
                     /locus_tag="AC16V7_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016781547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HIR6256296.1"
                     /translation="VVAVTGGKGGVGKTNVSVNLSVALSQAGRRVVLMDADLGLANVD
                     VLLGLKPTHNIANLLAGECGLRDIMVEAPGGLRIVPASSGTQALVNMSAHEHAELIHA
                     FSEMEDEIDVLVVDTAAGISESVVSFVRAAQEVLVVVCDEPTSITDAYALIKLLNRDC
                     GMNRFRVLANMVRSEQEGRNMYAKLTTVTDRFLDVTLQYVGSIPYDESVRKAVQKQQP
                     VYRAFPRSPAAQAFSKLAQKVLAWPVLSAPRGHLEFFVERLVRMPNQR"
     gene            819..1535
                     /locus_tag="AC16V7_03915"
     CDS             819..1535
                     /locus_tag="AC16V7_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007021045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HIR6256297.1"
                     /translation="MYSQLESDSQAEVVERYAPLVKRIAHHLLARLPSSVVLDDLVQS
                     GMVGLLEAARKYDASKGASFETYAGIRIRGAIIDEVRRGDWTPRSVHRNGRRISESIQ
                     SVEAKTGRDAQDADVAREMGVSLDEYYALLRDSSSTRLFSIDELREGRDSTLADELVG
                     DQPDPQLEHQQQAFQRALAEAIGRLPEREQLVFSLYYDEELNLKEIGQVLGVSESRVS
                     QIHSQAALRLRARLQTWGGA"
     gene            1626..>1879
                     /locus_tag="AC16V7_03920"
     CDS             1626..>1879
                     /locus_tag="AC16V7_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020209487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6256298.1"
                     /translation="MKILIVDDFSTMRRIIKNLLRDLGFNNTVEADDGSTALPVLKNG
                     GIDFLVTDWNMPGMSGLDLLEQVRADPALAKLPVLMVTAEA"
BASE COUNT          407 a          452 c          584 g          436 t
ORIGIN      
        1 gtagttgcgg tcaccggagg caagggcggg gttggcaaga ccaacgtatc ggtcaatctg
       61 agtgtggcgt tgtcccaggc gggtcgtcga gtggtgttga tggatgctga tcttggcttg
      121 gctaacgtgg atgtgctgct ggggctgaaa ccgacccata atatcgccaa tctgctggcg
      181 ggagagtgtg ggctgcgcga tatcatggtg gaggcgccgg gcggcttgcg tatcgtgcca
      241 gcctcgtccg gtacccaagc gctggttaat atgagtgccc acgagcacgc cgagctaatt
      301 catgccttca gcgaaatgga ggatgagatc gacgtgctgg tggtggatac cgctgcggga
      361 atttcggaat cggtggtgag ttttgtgcgc gcggcacagg aggtgctggt agtggtgtgc
      421 gatgagccta cctcgattac cgacgcctac gcgctgataa agttgttaaa tcgtgattgc
      481 ggcatgaatc gctttcgtgt gttggctaat atggtcagaa gcgaacagga aggtagaaat
      541 atgtacgcaa agttgaccac ggtcacagac cggttcctag atgttacgct tcaatatgta
      601 ggatcaatac cttatgatga gtcggtcaga aaggccgttc aaaaacagca gccggtttac
      661 cgggcgtttc cacgcagtcc cgctgctcag gcctttagta aattagccca gaaagtcttg
      721 gcctggccgg tattgtctgc cccgcgaggg caccttgaat tttttgtcga gcgactggtt
      781 cgaatgccaa accagcgttg atgcctttaa gggtgttcat gtacagccag ctagaatcgg
      841 attcgcaggc agaagttgtt gagcgctatg cgccgctggt caagcggatc gctcatcact
      901 tgttggcgcg cctgccctct agcgtggtac tggatgacct cgtccagtcg gggatggtgg
      961 ggctcttgga agcggcgcgt aagtacgatg ccagcaaagg tgccagcttt gaaacctatg
     1021 ccggcatccg gattcgcggc gccattatcg atgaggtgcg ccgtggtgat tggacgccac
     1081 gctcggtgca tcgcaatggt cgacgcatct ccgaaagtat tcaatccgtt gaagcaaaaa
     1141 ctggtcgtga tgcacaagat gccgatgtgg cgcgtgagat gggcgtcagt ctggatgagt
     1201 actatgcctt gttgcgcgat agctcctcca cgcggctgtt tagtatcgat gagctgcgcg
     1261 aagggcgcga ttccacgctc gcagacgaac tggtaggtga tcagccagat ccgcagctag
     1321 agcaccagca gcaagccttt cagcgggcct tggcggaagc gatcggccgc ttgcctgaaa
     1381 gggaacagtt ggttttctct ctttattacg acgaagagct caacctgaaa gagattggcc
     1441 aagtgctagg ggtgagcgaa tcgcgggtta gccaaattca cagccaagct gcgctacgct
     1501 tacgtgcacg tttacaaacc tggggcggag catagtaata tgctgcacac cctgctcctc
     1561 tgctaaatgt gataccgagg agttcgcaat tgacaggcta gacggaggtc gccttggaca
     1621 aaaacatgaa gattcttatt gtcgatgatt tttcgactat gagacggatc atcaaaaatc
     1681 tgcttcggga cctgggtttt aacaacaccg tagaagccga cgatggcagc accgctctgc
     1741 cagtgttgaa aaatggtggt atcgatttcc tggtcactga ctggaatatg cccggcatgt
     1801 cgggcctgga cctgctggaa caggtgcgtg ccgatcctgc gttggccaag ctgccggtgc
     1861 tgatggtgac cgccgaagc
//
LOCUS       DBNHOH010000221         5476 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_653489, whole genome shotgun sequence.
ACCESSION   DBNHOH010000221 DBNHOH010000000
VERSION     DBNHOH010000221.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5476)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5476)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5476
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_653489"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="AC16V7_03925"
     CDS             <1..880
                     /locus_tag="AC16V7_03925"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008443 - phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR6256299.1"
                     /translation="TDEVNDLTPEVLKNIEYLNINYLIPIGGDDTLSYAVRLYQEGMK
                     VVAIPKTMDNDVPGTDYCIGFSTCVTRTLQMTNSLRTSAGSHERILVLEVFGRYAGFT
                     ALLPTMAGAAHRCVIPEYKFKIDHLTELLVYDRLKNPSRYSVVLVSEGAMFEGGEMVF
                     QDRTTDAYGHAKLGGIGDLVSEKIKEIAPQFNNGKPINTITQKLGYMVRGGEPDAIDA
                     IVPMAYGNLALDLILNRIHGRMVVLKNGRYDNVPIDVVIAMKKAVNVKKFYNTERLRP
                     NFEQFEMMPLFIMTSD"
     gene            complement(1054..5094)
                     /locus_tag="AC16V7_03930"
     CDS             complement(1054..5094)
                     /locus_tag="AC16V7_03930"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR6256300.1"
                     /translation="MSFVHLHVHSEYSLLDGFSKVKKLVKRAKEMEMPALALTDHGTM
                     FGVIDFYNAATAAGIKPIIGMEAYLAARSMQERDPQEDKKSTHLLLLAENETGYKNLL
                     QIASAAQMQGFYYFPRIDHDFLAQHSEGLICTSGCMAAEIPRLIYQSNLEAARRQLDW
                     YYEVFGPERFFLELQSHQIQELDTINQHLMTLGKRYKARFVATNDVHYVNPQDARLQD
                     ILLAIQTSCVLTDPNRMRMSDTSYYLRSPQEMAQLFAEVPEAIHNTVLIAERCNVDLG
                     FKGYHLPVFEVPEGQTPVSYLRQQCEKGFVKRFGSRADNPVYRQRLEYELDIIHQMGF
                     DTYFLIVWDLCQFARDQGIWYNARGSAAGSIVAYALEITLVDPIEHGLIFERFLNPGR
                     ISMPDIDLDFRDDRRAEIMEYATKKYGEDKVAQIITFGTLGARAAIRDVGRVMDIPLN
                     EVDNVAKLIPNIPGKPMTISDALEEVPDFKKMYDTAPYLRDLIDTATEMEGVVRNAGT
                     HAAGVIITDKPLLEYIPLHRPTGSASSEDSPVKVVTQFEMSVLESLGLLKVDFLGLAT
                     LTIMARACDLILQRHGVELNLDNIPLDDPATYELMGRGETAGVFQVEGSGMRRYLIQM
                     KPKELANVIAMVALFRPGPMDFIPGYIRRMHGEEPVTYRHPALEPIFQETYGYPVYQE
                     QLMFAVMQLAGYTAPEADDLRKAVAKKQKEKLLKHRQKFVKGAVKNSVPEETAEAIFD
                     EWEEFARYGFNKAHAADYGIIAVQTAYLKAHYPVEYMTAVLSVNLSDTAKVALYVADC
                     RRLGIAIEPPDVNFSGWDFTIEDHPENSTSTIRFGLGAVKNVGHGPVDAILQGRDDQT
                     FVDLDDFAHRVDLRQVGKRALESLIKVGALDSFGPRPALLEGIDRIISVSAAHFRAAD
                     MGQMSLFGVHTGVSETISLPQLVGEISRREMLNWERELIGLYVSDHPLSPVMNEITQA
                     VTHFSAQLPEVAANEKVRVAGVIVRIRPHQSKAGKPMGFVAIEDLQGTIELVIFPSVW
                     SRVSTIIEYDRIILVDGRVDSSGAEPKVLVDNVTTDLNMKVAVQEDKASRRNNETTNR
                     QARKLEDRKIDAKEESLSEEDLLLESDSSNSSFNDFPDFDDDVPPMPDPPSDWDIYEP
                     EEPSIAAIAVGLFAGGENGELGVDTATVSDVMSDGNVQDAATGDQLVGEQAPSVEEAT
                     EQKGMPGETPSTQDVKPDHAPEKTTEQATERLPAKPSDPAVLLPPFLVRPPISSAREQ
                     VQMITVILRSTGDKTRDVLRLRRIHGTLMSYPGNDRFAFHVFERERGYLLEFPNFTTD
                     VSAELIAKLQALVGADNIRLESITFQ"
BASE COUNT         1318 a         1484 c         1424 g         1250 t
ORIGIN      
        1 cacagacgag gttaatgatc tcacccctga ggtgttaaag aatattgagt accttaacat
       61 caattacctc atcccaatcg ggggtgacga caccttgagc tacgctgtac gcctgtatca
      121 agaaggcatg aaagtagtag ccatacccaa aacgatggat aatgatgtcc caggcaccga
      181 ttattgtatc ggcttcagca cctgtgtcac ccgcacactc cagatgacca atagcctgcg
      241 cacatcggct ggatcgcatg agcgcatcct ggtgctggaa gtattcggtc gctatgctgg
      301 tttcaccgcc ctactcccca cgatggcggg ggcagctcat cgctgtgtga tcccagagta
      361 caaattcaaa attgaccact tgaccgaact cctggtgtat gaccgcttga aaaaccctag
      421 ccgttattca gttgtgctgg tatccgaagg ggctatgttt gaagggggcg aaatggtttt
      481 ccaggatcgc acaaccgatg cctacggtca cgccaaactg ggtggaattg gcgatttggt
      541 ttctgagaag atcaaagaga ttgcacccca attcaataat ggcaaaccaa ttaatacgat
      601 aacccaaaag ctcggttaca tggtgcgcgg tggcgagccg gatgctatcg atgccatcgt
      661 acccatggcg tatggcaacc tggctttaga tctgatacta aaccgtatcc atgggcggat
      721 ggtcgtcttg aaaaatgggc gttacgataa cgtacccatc gatgtcgtca tcgccatgaa
      781 aaaagccgtg aacgtgaaga aattctacaa cacagagcgg ttacggccaa actttgagca
      841 atttgagatg atgccgctct tcatcatgac cagcgactaa cccaattagg gctacgcccg
      901 cagggcgatg ccgccctccc ccgctggaga gcgagaaata tatcggaaaa acgggctatt
      961 gggcggcggg aatgccgccc aatagcccgt ttttccgtaa atcatccccc tcccgccgct
     1021 ttggcggaag ggaggggaac gacttgttct cttctattga aaagtaattg attctaagcg
     1081 gatgttatcg gcgcccacca gagcctggag tttggcaatc aactcggcgc tcacgtccgt
     1141 cgtaaagtta gggaactcta acaggtagcc tcgctcgcgc tcaaatacgt ggaaagcgaa
     1201 gcgatcatta cccggatagg acatgagcgt gccgtggatg cggcgcaagc gcagcacatc
     1261 gcgagtcttg tcccccgtag aacgcagtat caccgtgatc atctggactt gctctcgtgc
     1321 agatgagatg ggaggccgta ccaggaaagg cggcagcaac accgcaggat cggatggttt
     1381 cgctggcagt cgctccgttg cttgttcagt ggttttctca ggagcatggt cgggctttac
     1441 atcttgtgtg gagggagtct cacccggcat acccttctgc tcagtggcct cctccacaga
     1501 gggcgcttgc tccccgacca actggtctcc tgtagcagca tcctgcacat ttccatcaga
     1561 catgacatcg ctcactgtgg ctgtatccac ccccaactct ccgttctctc caccagcaaa
     1621 gaggcctacc gcgatcgcag ctatgcttgg ttcttcgggc tcgtatatat cccagtctga
     1681 aggcgggtca ggcatgggag gtacatcatc gtcaaagtct ggaaagtcgt tgaatgacga
     1741 attcgaagag tcactttcaa ggagtaaatc ttcttctgac agactctctt ctttcgcatc
     1801 gattttccga tcttccaact ttcgagcctg tcgattagtc gtttcgttgt tacgccgact
     1861 cgctttatcc tcctggacag ctactttcat atttaaatca gtggtgacat tatccaccag
     1921 cacctttggc tcagcgccgc tagaatccac gcgcccatcg acaagaatga tacgatcata
     1981 ttcgattatt gtggagactc tcgaccagac gctggggaag atgaccagtt cgatagtccc
     2041 ctgcaaatct tcgatagcta caaagcccat tggcttgcca gccttgcttt gatgagggcg
     2101 aatacgtacg atcacacccg caacccgtac tttttcattc gccgctacct caggtagctg
     2161 agctgagaaa tgggtaacgg cctgggtgat ttcgttcatc accggagaga ggggatgatc
     2221 ggagacatac aggccgatca actcacgctc ccaattgagc atttctcggc ggctgatttc
     2281 cccaaccaat tgcggcaagc taatggtctc agagacacct gtgtgcacac caaaaaggct
     2341 catctggccc atatctgctg cacgaaaatg ggccgcgctg acagagatga tgcggtcaat
     2401 gccttccaaa agggcagggc gcgggccaaa gctgtccagc gcgccaacct tgatcaagct
     2461 ctctaaagcg cgcttgccga cctggcgcaa atccacgcga tgggcgaaat cgtccagatc
     2521 aacaaaggtt tgatcatcac gcccctggag gatcgcatcc accgggccgt gccccacgtt
     2581 cttgaccgcc ccaaggccaa aacggatggt tgaggtgctg ttttcggggt gatcttcaat
     2641 ggtgaaatcc cacccgctaa agttgacatc tggcggctca atggcaatac ccaagcggcg
     2701 gcaatccgcc acataaagag ccaccttggc agtgtcgctc aggttgaccg acagaaccgc
     2761 cgtcatatac tcaaccgggt agtgagcctt caggtaagcg gtttgaaccg caataatgcc
     2821 ataatcagcc gcgtgggctt tgttgaaccc atatcgagca aattcttccc actcgtcgaa
     2881 tatggcttca gctgtctctt cagggacgct gtttttaact gcccccttta caaacttctg
     2941 gcggtgttta agcagcttct ctttctgctt cttggcgacc gctttgcgca agtcgtcggc
     3001 ctctggggca gtgtaacccg ccagctgcat tacagcgaac atcaactgtt cctggtagac
     3061 tggatatcca tacgtctcct ggaaaattgg ctctagtgca gggtggcgat aggtcacagg
     3121 ctcttcccca tgcatgcggc ggatgtagcc agggatgaaa tccatcggtc caggacgaaa
     3181 caaagctacc atggcgatca cgttagcaag ctctttgggt ttcatctgaa tcaagtaacg
     3241 gcgcatcccc gacccttcca cctggaacac accggcagtc tcacccctgc ccatcagctc
     3301 atacgtcgca ggatcatcca gcgggatatt atccaggttc agttccaccc cgtgtcgctg
     3361 gaggatcagg tcgcaagcgc gtgccatgat ggtaagcgtg gctaatccca agaaatccac
     3421 cttgagcagc cccagcgact cgagcaccga catctcaaat tgggtgacca ctttcaccgg
     3481 gctgtcttcg ctggatgcgc tcccggtagg ccggtggagc gggatatact cgagcaaggg
     3541 cttatcggtg atgatcactc cggccgcgtg cgtgcccgcg ttacgcacca ccccctccat
     3601 ttcagtggct gtatcgatca aatcgcgcag atagggagct gtgtcataca ttttcttgaa
     3661 atcggggact tcttccaacg catctgaaat ggtcatcggc ttgcccggga tattagggat
     3721 cagcttggca acgttatcca cctcattcag gggaatatcc atcacgcgac ccacatcgcg
     3781 tatggcggcg cgggcaccca gtgtgccaaa cgtgatgatc tgcgccacct tatcctcgcc
     3841 atactttttt gtggcatact ccatgatctc cgcccggcgg tcatcgcgaa aatccaggtc
     3901 aatatcgggc atggaaatac gccctgggtt gagaaagcgc tcgaagatca agccatgctc
     3961 aatgggatcc accagggtga tttcgagggc ataagctaca atcgatccgg ctgctgagcc
     4021 gcgggcgtta taccaaatac cctggtctcg ggcgaactgg catagatccc aaacgatcaa
     4081 aaagtacgta tcgaagccca tctggtgaat aatatccagc tcatattcca agcgctggcg
     4141 atagaccggg ttatcagcac gcgagccgaa tcgcttgaca aaacccttct cgcactgctg
     4201 gcgcaggtag gatacagggg tttgaccttc aggtacctcg aaaacgggca ggtggtagcc
     4261 cttgaaaccc agatcaacat tgcaccgctc tgcgatcaga accgtattgt gaattgcctc
     4321 cggcacctcg gcgaaaagct gtgccatttc ttgcgggcta cgcaggtaat agctagtatc
     4381 actcatgcgc atgcggtttg gatcggtgag cacgcaactg gtctggattg ccagcaggat
     4441 atcctgcaga cgggcatctt gtggatttac ataatggaca tcattagtgg caacgaagcg
     4501 cgctttgtag cgcttgccca atgtcatcag gtgctggtta atggtgtcca gctcttgaat
     4561 ttggtggctt tgaagctcca ggaaaaagcg ctccgggccg aagacctcgt aataccagtc
     4621 caactggcgg cgggctgctt ccaggttaga ttgatagatc aagcgcggta tttcggctgc
     4681 catgcaccca gaagtgcaga ttaaaccctc ggagtgctgc gccaggaaat catgatcgat
     4741 acgcggaaag taataaaaac cctgcatctg ggccgcgctg gcaatctgga gcagattctt
     4801 gtagccagtc tcattttctg ccagaagcaa caggtgagtg gattttttat cctcctgagg
     4861 gtcgcgctcc tgcatcgacc tggcagccag gtaggcctcc atgccgatga taggcttgat
     4921 gccagcggca gtagcggcgt tataaaagtc gatcacgccg aacatggtac cgtggtcagt
     4981 gagcgccagg gcgggcattt ccatctcctt ggcgcgcttg accagcttct tcaccttgga
     5041 gaagccatcc agcagggagt attcggaatg gacatgcagg tggacgaagg acatgatttg
     5101 cagtggtcag ccaacaaaac gaaacgaaca aaaccctaat ggatatgaat tggtttgcga
     5161 gatgcacgca gggcaaaaaa aatctagccg tgaaaggatt tccgggcttg gagagattat
     5221 agcacggcga gtgcagaacg gaggtttaac caaccttaaa atttcgccag gataagggtg
     5281 ctacagtaat ccaccgaaat tggaaaccac ttatgtgcaa aaacgtcccg cagatttctc
     5341 taaaacaagc ctgtgtgctc ataaacctgc gggacgttca tctgggtaac ccaatttgcc
     5401 cttaaggctt acggatgaaa ggcaggtaaa ttaactctgg gggctcagcc gacccgcagg
     5461 tgtagatgcg gatatc
//
LOCUS       DBNHOH010000222         1124 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1679694, whole genome shotgun sequence.
ACCESSION   DBNHOH010000222 DBNHOH010000000
VERSION     DBNHOH010000222.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1679694"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="AC16V7_03935"
     CDS             complement(<1..364)
                     /locus_tag="AC16V7_03935"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR6256301.1"
                     /translation="MFFAECLDCGHQYHYSPHTVTCPNCNGQWCEGRYDYPALGKIFP
                     SLLKERPFNLWRYRELLPIYEYNQSFSMGEGGTPLVHAANLGMMLGCPNIYIKDERQG
                     PTRSFKDRQAAVGIASLKE"
     gene            complement(406..>1124)
                     /locus_tag="AC16V7_03940"
     CDS             complement(406..>1124)
                     /locus_tag="AC16V7_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6256302.1"
                     /translation="FTEQGSITLRAYTQSGLGEQPQLYISVTDTGPGIAPEDREKLFQ
                     PFSQVDASATRKAGGSGLGLSISRHLVEMHGGQIGVESELGKGSTFYFTLPIPQKNGF
                     FSEDTNPTLTNNPLVLTIDSEMSILQHYERYLMNNGYQAYALTDPLKAIELAIKLHPM
                     AIILDVLIPNFDGWQLLKDLKTDPNTQHIPVIICTLLDNPSKGFSLGADQYLMKPVLE
                     DDLVHALKRFSNNVSRQNNM"
BASE COUNT          283 a          252 c          285 g          304 t
ORIGIN      
        1 cctctttcag tgaggctatc cctacagccg cctggcgatc tttaaatgat ctcgtaggac
       61 cttgccgctc atccttgata tagatattgg gacaacccaa catcattcca agatttgcgg
      121 catgcactaa tggggtacca ccttccccca tagaaaatga ttgattgtat tcgtagatgg
      181 gcagaagctc acgatagcgc cacaggttaa agggacgctc cttgagtaaa gacggaaaga
      241 ttttgcctag tgctggataa tcgtagcgcc cctcgcacca ctgaccgtta caatttgggc
      301 atgtgaccgt atgaggtgaa tagtggtact ggtggccaca gtcgagacat tcagcgaaaa
      361 acatggacga tggcggagac tgtgtggtgt gattcaaagt tggctttaca tgttgttctg
      421 acgtgatacg ttgtttgaga accgcttgag cgcatgcacc agatcatcct ctaaaacagg
      481 tttcattagg tattggtccg cgcccaggct gaaacctttt gatggattat ctaatagggt
      541 gcatatgatg acagggatgt gctgagtatt cgggtcggtc ttcaaatcct tcagtagttg
      601 ccatccgtca aaattgggga tcaagacatc caggatgata gccattggat ggagcttaat
      661 tgccaactca atagccttca agggatctgt cagagcgtag gcttggtagc cattgttcat
      721 gagataacgc tcgtaatgct gcaggatgga catttcactg tcgatcgtaa gaaccagagg
      781 gttgttggta agggttggat ttgtgtcttc cgaaaaaaat ccatttttct gcggaattgg
      841 cagagtgaaa tagaaagtgc tccctttgcc taactcactt tctaccccta tttgacctcc
      901 atgcatttcc accaggtgac gggagatgga taagcctaat ccgctaccgc ctgctttgcg
      961 cgtagccgat gcgtccacct gggaaaaagg ctggaataac ttttcgcgat cttcgggggc
     1021 aatacctgga ccagtatccg taacacttat ataaagctga ggctgttctc ccaatccgct
     1081 ttgggtataa gcgcgaagag tgattgaccc ttgctcggta aatt
//
LOCUS       DBNHOH010000223         1972 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_525649, whole genome shotgun sequence.
ACCESSION   DBNHOH010000223 DBNHOH010000000
VERSION     DBNHOH010000223.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_525649"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1415
                     /locus_tag="AC16V7_03945"
     CDS             <1..1415
                     /locus_tag="AC16V7_03945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR6256303.1"
                     /translation="FSYVVSLLRPEIIRELDLPKHGLAILPLESTLTPLPDGDYLYRD
                     ADHYRNMREIARFSPRDADAYEDYSHTMYFMAKAVKYILGIHPPNPASLHPNDLAGLY
                     RLARHFLNLPGEQIHTLARLMTMSSADFLERWFESEPLKGTLSASGIIGTFLGPRSPG
                     TAYVLLHHYMGEIDGNFRSWGFAKGGTGGISQAIAGAARSLGAEIRLNSPVSEVIVKG
                     GRAVGVALRDGKEIAAKVVVSSLDPNQTFLKLVDPNVLPNDLVSSVRKYVYRGSSGKV
                     NLALDAAPQLARMPDGGAALRGAISISPSVDYIERAYDQAKYGDFSQRPYIDIIIPSM
                     IDPNMAPPGKHVMSCFVQYASYHLQEGSWEQRREAFGDALVDTLSEYIPNLKDIIVYR
                     QVITPYDIEQSIGLTEGNIFQGELSLSQLFFLRPAPGYANYRTPVKGYYQCGSATHPG
                     GGISGAPGRLAALRILKDLR"
     gene            1412..>1972
                     /locus_tag="AC16V7_03950"
     CDS             1412..>1972
                     /locus_tag="AC16V7_03950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024842.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR6256304.1"
                     /translation="MMSTVSKGEEFDAVIIGAGHNGLVTAAYLAKAGLRVVVLERREV
                     LGGAAATEEIFSGYQINTGTCDTSLLLPKIISDLDLETNGVEFIHPPAAIFAPQLDGR
                     PLTIWRDPQRTREEIAAFSKKDADNFPGYLRLVSRLGEILRDTMTLTPPAIPEITFGS
                     VLPWLPLALKVKRLGDKDLTELLRVVP"
BASE COUNT          490 a          481 c          490 g          511 t
ORIGIN      
        1 tcttctccta tgtagtaagc ttgttgaggc ctgaaattat ccgcgagtta gatcttccaa
       61 agcacggcct cgccattctt ccattggaga gcacccttac acccttgccg gatggagatt
      121 atctatatcg agacgcagat cattatcgca acatgcggga gatcgctcgt ttttccccca
      181 gagatgctga tgcctatgag gattattccc acactatgta ctttatggca aaagcggtaa
      241 agtatatcct ggggatccat ccacctaatc ctgccagctt gcatcccaat gatcttgctg
      301 ggctttaccg gctcgctcgc cacttcctca acctgccagg tgaacaaatc cacacactcg
      361 cccgactgat gaccatgagt tcggcagact tcttggagcg ctggtttgag tcggagccac
      421 tcaagggaac cctctctgcc agcggcatca ttggcacatt tttagggccg cgctcaccag
      481 gtacagctta cgtgctcctc catcattaca tgggtgagat cgatggcaac tttcgctcct
      541 ggggttttgc gaaaggtggc acgggtggta tttcgcaagc tattgccggc gctgcgcgtt
      601 ccttaggagc ggagatacgc ctaaattctc ccgtaagtga ggtgatcgtc aaaggcgggc
      661 gggctgttgg tgtagctcta cgggatggca aagaaatagc cgccaaggta gttgtatcta
      721 gtctggatcc caaccaaacg ttcttaaaac tagtcgatcc aaatgtcttg cccaatgact
      781 tggtctcctc ggtgcgcaaa tatgtatata ggggatcttc cggcaaggtt aatctagccc
      841 ttgatgctgc cccacaatta gcccgtatgc cagatggggg cgctgcattg cgcggggcga
      901 tttcgatcag ccccagcgta gattatattg agcgcgccta tgatcaggca aagtatgggg
      961 atttctcgca acgcccctat atagatatca taatcccatc catgatagat ccaaatatgg
     1021 cgccaccggg caagcatgtc atgtcttgct ttgtgcaata tgcatcctat cacttacaag
     1081 aaggctcgtg ggagcagcgc cgagaagcat ttggtgatgc actagtcgat acgctatccg
     1141 aatatattcc gaatctaaag gacatcatag tataccgaca ggtgataacc ccttatgata
     1201 ttgagcagtc tattggttta accgagggaa atatcttcca aggggagctc agtctctcgc
     1261 agctcttctt tttacgacca gctccaggat atgcaaatta tcgcactcca gtcaaaggct
     1321 actaccaatg tggttctgct acccatccag gaggagggat ctcgggtgca ccgggtcgcc
     1381 tagcagcatt aagaatatta aaggatctac gatgatgagc actgtttcaa agggtgaaga
     1441 atttgatgct gtaatcatcg gtgcagggca taatggactg gtgacagcgg cttatttagc
     1501 caaagcagga ctgagggttg ttgtactaga acgaagggaa gtgttgggtg gtgcagctgc
     1561 aacggaggaa attttctccg gttatcaaat taatacaggg acatgcgata caagcctatt
     1621 gttgcccaag atcatctcag atctcgatct agaaaccaat ggtgttgagt tcattcaccc
     1681 ccctgcagca atatttgcgc ctcaattaga tggtcgtcca ctgacgatct ggcgagatcc
     1741 acaacggact agagaagaga tcgctgcttt ttcgaaaaag gatgcggata atttccctgg
     1801 ttaccttcga ttggtttctc gattggggga aattctgcgg gatacaatga cactcacccc
     1861 tccagcgatc ccagaaataa cttttggatc ggttctaccc tggttgccat tggcattgaa
     1921 agttaaacgc ttgggtgata aagatctcac agaactattg agagttgtgc cc
//
LOCUS       DBNHOH010000224         6187 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1743642, whole genome shotgun sequence.
ACCESSION   DBNHOH010000224 DBNHOH010000000
VERSION     DBNHOH010000224.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6187)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6187
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1743642"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="AC16V7_03955"
     CDS             <1..281
                     /locus_tag="AC16V7_03955"
                     /inference="COORDINATES: protein motif:HMM:NF019632.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HIR6256305.1"
                     /translation="AEGYGWEQEDDLNQNIYEKYRGWSLDEVLAYYRESFQETVAAVQ
                     VMTEEELFTPGRYAWTKNNTLADYVVPCTSEHYQWARQEMSAGLKPGR"
     gene            283..1302
                     /locus_tag="AC16V7_03960"
     CDS             283..1302
                     /locus_tag="AC16V7_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifH"
                     /protein_id="HIR6256306.1"
                     /translation="MMAWQDLLHGDSLSWLLEPDSPGVRYLALRDLVDLPHDDPALLA
                     ARAAAHTQGPIAAILSEMDESGYWAKSGPGYLPKYRSTVWSIIMLAQLGALAENDPRL
                     ERACAYLIDHALASGGQFSASGAPSGTADCLQGNMCAALLDLGYDDPRLEAAFEWMAR
                     TLTGEGIAPNSERDAPVRYYASKCGPLFACGSNNKLSCAWGGVKVMLAFARWPASRRT
                     ALTERAIQQGVDFFFSTDPAKADYPSGFSDKPSGNWWKFGFPVFYVTDLLQIVEALVA
                     LGYAADSRLSNAIELIRQKQDKHGRWALEYSYTGKTWVDFGPKKQPNKWVTLRALRVL
                     KATGD"
     gene            1311..1805
                     /locus_tag="AC16V7_03965"
     CDS             1311..1805
                     /locus_tag="AC16V7_03965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HIR6256307.1"
                     /translation="MKNSSQPPKLIWRVMRKLNLRIARNYLRGIGPGSIVLLLTTTGR
                     KSGLPRLTPLQYEEYEGVYYIGSARGQQADWFRNIQANPQVEVQVRRKRFPGLAEPIT
                     DPKRIADFLEMRLRRHPLMIGALMLLEGLPLRHTRADLERIAAQKALVIIRPILGSAS
                     STDR"
     gene            complement(1921..2094)
                     /locus_tag="AC16V7_03970"
     CDS             complement(1921..2094)
                     /locus_tag="AC16V7_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HIR6256308.1"
                     /translation="MAKDPVCGMDVDPKTAQWRSEYKGQMYYFCAPGCKRSFEKDPEK
                     FLSKLGDPAHHVH"
     gene            complement(2217..2666)
                     /locus_tag="AC16V7_03975"
     CDS             complement(2217..2666)
                     /locus_tag="AC16V7_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR6256309.1"
                     /translation="MKRVFYLLVLLCLIALLSACGGGSSQNADAVQQGEVAGEKVKVA
                     GGAYTDVDVAELQSMLANKDFTFVNVHIPFEGDIANTDVSIRYDQIEQNLSLLPPEKD
                     AKIVLYCRSDRMSNIAAETLVSLGYSNVYNLKGGMVAWERAGLPRVK"
     gene            complement(2758..4485)
                     /locus_tag="AC16V7_03980"
     CDS             complement(2758..4485)
                     /locus_tag="AC16V7_03980"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6256310.1"
                     /translation="MVETNPRPYSPWRGLTLQVFVLTILPLTVLLLFITVNSLSVHQR
                     AMRALVGERDERAVRTASRALESEIFHRASAVRSLALRAGNADSTALDYILSSSAFLQ
                     ADFDGGLAFFDQQGRLLSFTGETSLWENMASQGTTWIEAVLSSDAFPAYISPTFRDQI
                     SSEAMVLVAASAESTEIIAVGVFSPGSLVGRALEGAFASESQIGILLVDASGNALYES
                     GSLSSGEGIGAYSGVEEALRGKAGTIYIGSGDDEHVVAYAPVEPVGWAIVTEESWELV
                     TTPTLRTSQVMPLALVPALLIAVVGLWFGIRQVVQPIQKLEEKAARLAWGDFGSIEEP
                     VGGIPEVQHLQVELAQMARKLRAAQKGLHDYIGAITAAQEEERRRLARELHDDTIQSL
                     IALKQRVQLTRLSIKERQAADSMAELEGLAEQTIDNLRRLTRALRPIYLEDLGLVTAL
                     EMLARETSQSAGVPVTFHLDGSERRLPPEVEMAFYRITQEALSNVIRHSKASEASVRL
                     HYQPGSVSVGISDNGQGFEVPRSPAEFVPRGHFGLLGLHERAELLGARLEIRSAPGKG
                     ASIIVDMPT"
     gene            complement(4502..5179)
                     /locus_tag="AC16V7_03985"
     CDS             complement(4502..5179)
                     /locus_tag="AC16V7_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6256311.1"
                     /translation="MSSQTPPIRILLADDHAVVRAGIRQILEHAGEIQVVAEAGDGEA
                     AQAYIQQYQPDVAVLDIQMPKVTGIDVTRWARAQKIKVGILILTAYDDDPYVMAVLQA
                     GANGYVLKTASPDELIQAVRDVHAGKSALDAVVTQKLMSHMFQPRDTLPVEPLTEREM
                     EVLQLAARGFTNKAIGAQLGISDRTVQGHLAHIFAKLQASSRTEAVMRAVSLGWISQS
                     IGEIKDD"
     gene            complement(5246..>6187)
                     /locus_tag="AC16V7_03990"
     CDS             complement(5246..>6187)
                     /locus_tag="AC16V7_03990"
                     /inference="COORDINATES: protein motif:HMM:NF044972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR6256312.1"
                     /translation="ILNTLRLPSYWLIVVLSLDYALRRLDFITARWGEQVDSLLYILY
                     LLAGFTFAWSFIQNMFAWYGEEIATRTETDLDQQLMPFFRRVALIILTAVGLITLLGY
                     FDVEIGALVTTLGIGSLAIALAAQAALSDTISGFIIMIDRPFRIGDRIEIQDLDTWGD
                     VIDIGLRSSRIRTRDNRMVIVPNSVLGKSLIVNYSYPDSQYRIQIQVGVAYGTDLEKA
                     RKVMVEAVSNVEGVLTDHPVEALFLEFGPSALIFRVRWWIDSYVDTRRMFDKVNTALY
                     NGLTEAGIQIPAPQVDVHHHFKPGDASRAADFMPPRA"
BASE COUNT         1429 a         1794 c         1624 g         1340 t
ORIGIN      
        1 ctgctgaagg gtatggatgg gaacaggagg atgacctcaa tcagaatatc tacgaaaaat
       61 accggggctg gtcgctggac gaagtgctgg catattaccg cgaatccttc caggagactg
      121 tggcagccgt gcaggtcatg acagaagaag agttgttcac ccccgggcgg tatgcctgga
      181 caaagaacaa cacactggcg gattatgtag ttccttgcac gagcgaacat taccagtggg
      241 cacgccagga aatgagcgca gggctaaagc ccgggaggtg agatgatggc atggcaagat
      301 ctgttacatg gggactcgct ttcttggcta ttggagcccg attcccccgg agtccgctac
      361 ctggccttac gcgatctagt cgatctaccc cacgacgacc cggcgctatt ggctgcgcga
      421 gcggccgcgc atacacaagg acctatcgct gccatcctat ccgagatgga tgagagcgga
      481 tactgggcaa agtcaggccc tggctatctc cccaagtatc gctctacagt ctggtcaatc
      541 atcatgctgg cgcagttggg cgcgctagcc gaaaatgacc cgcgcctgga acgcgcctgt
      601 gcctacctga tcgatcatgc cctggcatct ggcgggcaat tttccgccag cggggcgcct
      661 tctggcactg ctgactgtct gcaaggcaac atgtgtgctg ccctgctaga cctgggctac
      721 gacgacccgc ggctggaggc ggcttttgaa tggatggcgc gcaccctgac cggcgaaggt
      781 attgccccga actccgagcg cgatgcccct gtgcgctatt atgctagcaa gtgcggaccg
      841 cttttcgcct gcggctcgaa caacaagctg tcctgcgctt ggggtggggt caaggttatg
      901 ctggccttcg ctcgctggcc ggccagccgc cgcactgccc tcaccgagcg agccatccag
      961 cagggtgttg actttttctt cagcaccgac ccagccaaag ccgattatcc atccggattc
     1021 agcgataaac ccagcggcaa ctggtggaag tttggcttcc cggtattcta cgtcaccgac
     1081 ttgctgcaaa ttgtggaagc gctcgtcgct ttgggctacg cggctgacag ccgcttgtcg
     1141 aatgccatag agctcatccg ccagaaacaa gacaagcacg ggcgctgggc gttggaatac
     1201 agctacaccg gcaagacctg ggtcgacttt ggtccgaaga agcaacccaa taaatgggtg
     1261 accttgcgcg ctttgcgcgt cttgaaggca acaggggatt aaatgcctta atgaagaatt
     1321 cgagccaacc cccaaagctg atctggcgcg tgatgcgtaa gctgaacctg cgcatagcac
     1381 gaaactacct gcgaggcatt ggcccaggta gcatagtgct gctgctcacc accaccggca
     1441 ggaaatcagg cttgccgcgc ctgacgcctc tccagtacga ggaatatgag ggggtttact
     1501 acattggctc agcgcgaggc cagcaagctg actggttccg caatatccag gcaaacccgc
     1561 aggtcgaggt gcaggtgcgc cgcaagcgct tccctgggct ggcggagccg atcactgatc
     1621 cgaaacgcat cgccgacttt ttagaaatgc gcctgagacg ccaccccttg atgatcggcg
     1681 ctctgatgct cctggaaggg ctgccgctca ggcacacccg tgctgattta gagcgcattg
     1741 cggcgcaaaa agcgctggtc atcatacgac cgattcttgg ttcggcgtca tctacagaca
     1801 ggtagcgaat aaaagtcaca tagtggggga agtaatcgat gcagttaggc aagggaatac
     1861 aagccaaaat ttgatagtct gggaggcttc gctgctcaat ctatcaaatt ttggctatgt
     1921 ttagtgaacg tggtgggccg gatctccaag cttgctcagg aatttttcgg ggtctttttc
     1981 aaatgaccgc ttgcagcccg gggcacagaa ataatacatc tggcccttgt attccgatct
     2041 ccattgagcg gtcttggggt cgacgtccat tccacatacg gggtctttag ccattggcgc
     2101 aatctccttt ctagctgact tctattgacc tacagaattt ttggttatga acgtcccgca
     2161 ggtttaggat cacacaagct tgtttcaggg gaacctgtgg gacgttttta catagatcac
     2221 ttcacccgag gcaggccggc tctctcccag gctaccatac caccctttaa gttataaacg
     2281 ttgctatacc ccaaactaac cagcgtctcg gcagcaatgt tgctcatccg gtcgctgcgg
     2341 cagtacaaga cgattttggc gtccttctcg gggggcaaca gagatagatt ctgctcgatc
     2401 tggtcataac ggatggatac atcggtattt gcaatgtccc cttcgaaggg gatgtgcaca
     2461 ttgacaaatg taaaatcctt gttcgccagc atggattgca attcggcgac atcaacatcg
     2521 gtgtaggcac cgccggcaac cttcaccttc tcacccgcga cctccccctg ctgtacagcg
     2581 tccgcattct gtgatgatcc ccctccacag gctgaaagca aggcaatcaa acacagcaaa
     2641 accaaaaggt aaaatactct cttcatcgat catttactcc ttctacaaat ctagatctca
     2701 tcctgatatg cttgcttgag ggttttggca ccctcaattt ttttaactga acgataccta
     2761 tgtgggcata tccacgataa tgctggctcc ctttccaggc gcggaacgga tctccagcct
     2821 ggctcccagc aattcagcgc gctcatgcaa ccccaacaat ccaaagtgcc cgcgcgggac
     2881 aaactccgct gggctgcggg gaacctcgaa tccctggcca ttatcactta tccccacaga
     2941 cacagagccg ggttgatagt ggagccgcac actcgcttcg ctggccttgg aatgacggat
     3001 cacgttgctc aaagcttcct gggttatccg gtagaaggcc atctccactt ctggaggcag
     3061 ccttcgctcg ctgccgtcca aatggaatgt cactgggact ccagcgctct ggctcgtctc
     3121 tcgagccagc atctccagag cagtcaccaa acccaagtcc tccaagtata ttgggcgcag
     3181 ggcacgcgtc aatcggcgca ggttatcgat ggtttgctca gccagcccct caagctccgc
     3241 cattgagtca gcagcctgcc gctccttaat ggacagtcgc gttagctgga cgcgttgctt
     3301 gagtgcgatt aaggactgga tagtatcatc gtggagctcc cgcgccaggc ggcggcgctc
     3361 ctcttcctgg gcagctgtga tagcgccaat ataatcgtgc aagccctttt gggcggcgcg
     3421 cagtttgcgg gccatttgag ccagctcaac ctgcaggtgc tgcacttctg ggatgccgcc
     3481 gaccggttcc tcaatggagc caaaatctcc ccaggcgagc cgggcggctt tttcttccag
     3541 cttttgaatc ggctgaacca cctgccgtat gccaaaccac aagccaacca cagcaatcaa
     3601 caaggctggc accagggcca ggggcataac ctgcgatgtg cgcagggtag gggtagtcac
     3661 caactcccac gactcttcgg tgacgatagc ccagcccaca ggctctactg gtgcataggc
     3721 gaccacgtgc tcgtcatccc cagatccgat atagattgtc cccgccttgc cgcgcagtgc
     3781 ctcttctaca cctgaatatg cacctattcc ctcgccagat gataacgatc cgctctcata
     3841 cagcgcatta ccactggcat ccaccaaaag gatgccgatc tggctttccg aggcaaaggc
     3901 tccctccaga gcgcgtccta ccagcgaacc aggagagaag actcccactg caatgatctc
     3961 ggtggattcg gcactcgctg caaccaggac catcgcttca ctgctgattt gatcgcggaa
     4021 tgtgggtgag atgtaggcag ggaaagcatc ggacgagagc accgcttcga tccaggtcgt
     4081 cccttgagat gccatgttct cccacagact ggtctcccca gtaaatgaca gcaacctccc
     4141 ctgctggtca aaaaatgcca atcctccatc aaaatcagcc tggagaaagg cgctgctgga
     4201 tagaatataa tccagtgcag tggagtcggc gtttccagcc cgcagagcaa ggctgcgcac
     4261 tgcggatgcg cggtgaaaaa tttcagactc tagagcgcgc gaagccgtgc gtacggcgcg
     4321 ctcgtcccgc tcaccgacca gggcgcgcat agcgcgttga tgcaccgaca gactgttgac
     4381 cgtgatgaac aataacagca cagttagcgg caggatggtt aacacaaata cttgcaatgt
     4441 cagcccgcgc cagggagagt aaggccgagg gtttgtttcc accatgccag gtacctataa
     4501 gctaatcgtc tttgatctcc ccaatggatt gggagatcca ccctaaggaa accgcacgca
     4561 taaccgcctc ggtacggctg ctggcctgca gcttggcaaa tatatgcgcc agatggcctt
     4621 gcaccgtccg gtcactgata cccaattgag cgccaattgc cttgttggta aatcccctgg
     4681 cagcaagctg caacacttcc atctcccgct ctgtaagcgg ctcaactggc agcgtatctc
     4741 gcggttgaaa catgtgcgac atgagcttct gggtcacaac agcatccagg gcggatttgc
     4801 ccgcatgcac atcgcgcaca gcctggatca attcgtctgg agaggccgtt ttgagcacat
     4861 aaccatttgc gccagcctgc aacaccgcca tcacataggg gtcatcatcg taggctgtaa
     4921 ggatcaggat gcccaccttg atcttttgcg cccgcgccca gcgagtgaca tcgatgccgg
     4981 tgactttggg catttgaata tccagcactg cgacatccgg ctggtactgc tggatgtatg
     5041 cctgagcggc ttcaccatca cccgcctcgg caacaacctg gatctcccca gcgtgttcca
     5101 ggatctggcg aatgccagcc cgaacaacgg cgtggtcatc tgcaagcaga attcgtatgg
     5161 gtggcgtctg agaagacatt atcaataacg tatcctagcg gttacaagcc atactctctt
     5221 tatcaactcg catcactcta tctgcttatg ctcgtggagg cataaaatca gccgccctac
     5281 tcgcatcacc cggtttgaaa tggtggtgca cgtccacctg cggggcagga atttgtatac
     5341 cagcctctgt caacccgttg tacaatgcag tgttcacctt atcaaacatg cggcgcgtat
     5401 ccacataaga atcgatccac cagcgcaccc ggaagatcaa ggcggaaggt ccaaactcta
     5461 aaaacaaggc ttccactggg tgatcagtga gcactccctc cacgttactg acagcctcga
     5521 ccatgacctt tcgcgctttt tccaggtctg tgccatacgc gaccccaact tgaatttgga
     5581 tgcgatactg gctgtctgga tatgaatagt tgacaatcaa gctcttgcca agtaccgaat
     5641 ttggcacgat caccatgcga ttatcacgcg tccggatacg cgagctgcgc aggccaatat
     5701 caattacatc accccacgta tccagatcct gaatctcgat ccggtctccg atccggaagg
     5761 gtcggtcgat catgatgatg aatccgctga tcgtgtcaga taaagctgcc tgagccgcca
     5821 gcgcgatggc gagtgatcca atcccgaggg tagtgaccaa cgcgccgatt tctacatcga
     5881 aatatcccaa caaggtgatc agccctaccg cggtcaagat gatcaatgca acccgccgga
     5941 agaagggcat taattgttga tctaggtctg tttcagtccg tgtggcgatc tcctctccat
     6001 accaggcaaa catattctgg ataaagctcc aggcaaaggt aaaaccagcc aagagataga
     6061 gaatgtagag gagcgagtct acctgttcgc cccatcgcgc ggtaataaag tcgagcctgc
     6121 gcagggcata atcaagggat aagaccacta tcagccagta agacggcagg cgcagggtat
     6181 taagtat
//
LOCUS       DBNHOH010000225         1913 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1102632, whole genome shotgun sequence.
ACCESSION   DBNHOH010000225 DBNHOH010000000
VERSION     DBNHOH010000225.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1102632"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1226)
                     /locus_tag="AC16V7_03995"
     CDS             complement(213..1226)
                     /locus_tag="AC16V7_03995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR6256313.1"
                     /translation="MRKPVFIAILLLGVFFVITRMAEVEEIVRTSQQGDWRFFLLALT
                     AVLAWLINVAVSYKTIFQATGIDEKFTKLILLAAAANFVNVVAPSAGVGGMAVFVSEA
                     QRRGYSTGRVTMAGVLYILFDYAGFLCVLFLGLIVLIRRHNLTAAEVAATTLLVLIAA
                     AMAAFIYIGMQSSKMLRNVLVWMARRINRLLWPFIHRPYLSEERAHSFAQDAAAGLVE
                     LRRNPQSLITPFGLALSNKTLLISILFFVFLAYKTPFSIGTLVAGFSVAYLFLIISPT
                     PSGIGVVEGVMTLVLRSLYVPWGLAALITLTYRGFTFWLPLIFGLIAFRYISRSAEAK
                     IAI"
     gene            complement(1323..>1913)
                     /locus_tag="AC16V7_04000"
     CDS             complement(1323..>1913)
                     /locus_tag="AC16V7_04000"
                     /inference="COORDINATES: protein motif:HMM:NF016206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HIR6256314.1"
                     /translation="ARSRMSGAQAAQRLIERGGLYDVKIEGVQGNLTDHYDPRTRVLR
                     LSRGVYSGSSVASLAIAAHELGHAMQDQQDYMPLRLRSALVPAVNVGSYLGWIFIFIG
                     LLLRAPQLAWVGVIVFSGGALFALATLPVELNASARAKRLLVESGLIVGEDEARGVNA
                     VLNAAALTYVAALVTAVMQLMYYATLVLGMGGRRRG"
BASE COUNT          494 a          519 c          515 g          385 t
ORIGIN      
        1 caacttccgg acaagctcca acctgccgtt atactcaact gcacgctcag gtattcatat
       61 cagcgaccaa ttgaatgtta gcggggaagg tcagtgatcc tcgcgcctgg ctatgcggag
      121 ctcttcaagg tcagcaatca tagcttcatc gtgcatttgc atgcccgtgc cgtcaagagg
      181 agatgtgaag aaaacatgac ggggatgttg ctttatatcg ctatctttgc ctcggcgctg
      241 cgagagatat agcggaaggc aatcaagcca aaaatcaatg gcaaccagaa tgtaaatccg
      301 cgataggtca atgtgatcaa ggcggccagg ccccagggca catacagcga gcgaagcaca
      361 agtgtcatta ccccttcaac tacgccaatt cccgatggcg tcggcgatat gatgaggaaa
      421 agatatgcca cgctgaagcc ggccaccagc gtcccgatag aaaatggagt cttataagcc
      481 aggaaaacaa agaataagat cgagataagc aaggttttgt tgctcagtgc tagtccgaag
      541 ggcgtgatca ggctctgcgg gttgcgccgc agttctacca aaccggcggc agcgtcttga
      601 gcgaagctat gtgcccgctc ctcggagagg tacggtctgt ggataaaagg ccacaagagg
      661 cggtttatac ggcgcgccat ccagaccaat acgttgcgta gcattttgga agactgcatg
      721 ccgatgtaaa tgaacgcagc catcgcagcg gcaatcaata cgaggagcgt cgttgctgcc
      781 acttcagcgg cggtgaggtt gtgccgccgg ataaggacaa ttaatccgag aaatagcacg
      841 catagaaatc cggcgtaatc gaacaatata tacaatacgc ctgccatggt aacccgcccg
      901 gtggaatagc cccgccgctg tgcttcggag acaaacactg ccatcccccc cacccccgct
      961 gagggcgcca ccacattgac aaaattggct gcggccgcca gcaggatcaa tttggtgaat
     1021 ttttcgtcga tgcctgttgc ttggaatatc gttttatagg aaactgctac attgatcagc
     1081 caggccaaga cggctgtaag cgccaggaga aagaatcgcc aatcgccttg ctgggaggtg
     1141 cgcacaatct cctctacctc tgccattctt gtgatcacga aaaatacacc caaaagcaga
     1201 atggcgataa agacaggctt gcgcatgaaa ttattgtatg gaaattgcta ctcgattatc
     1261 ttaaattcag ggattagctt gggattctat aacacgtctt gatcccaagc tatccagcga
     1321 gcttaacccc ttctcctgcc gcccataccc aacaccaggg tagcgtagta cattagctgc
     1381 atcacggcgg ttaccaatgc agcgacatag gtaagtgcag cagcgttcaa tactgcattc
     1441 acgccgcgcg cttcatcttc tccaacaatc aagccggatt caaccagcaa acgcttggcg
     1501 cgggcggagg cattgagctc gacaggcagg gttgctaggg caaagagcgc cccacccgag
     1561 aagacgatca caccgaccca ggccagttgt ggtgcacgta agagcaagcc gatgaagatg
     1621 aagatccagc ccaggtatga gccgacatta actgccggga caagggctga gcgcaagcgt
     1681 agcggcatat aatcctgctg atcctgcata gcgtgaccca gttcgtgcgc ggcgattgcc
     1741 agggaggcca cggagctccc ggaatatacg ccccgcgaga ggcgcagcac ccgcgtgcgg
     1801 ggatcgtagt gatcggtcag gttgccttgt acgccctcga tttttacatc atataagccc
     1861 ccgcgctcga tcaggcgctg ggctgcctgg gcaccgctca tacggctgcg ggc
//
LOCUS       DBNHOH010000226         1932 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_140996, whole genome shotgun sequence.
ACCESSION   DBNHOH010000226 DBNHOH010000000
VERSION     DBNHOH010000226.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_140996"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            504..>1932
                     /locus_tag="AC16V7_04005"
     CDS             504..>1932
                     /locus_tag="AC16V7_04005"
                     /inference="COORDINATES: protein motif:HMM:NF052741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR6256315.1"
                     /translation="MVSRPHLISRLNAGMGVKFVLISAPAGFGKSTLMSEWARTIDKP
                     VTWLSLDENDNNLKRFLSYLIAALRQIDGSIGESILASLESTDNPPIELAITILINEI
                     AESGVEFSLVLDDYHLITSQKINEALNFFLDHLPPNAHLLISTRVDPPLPLSRYRARS
                     QMVEIRLDDLRFTEAEVRSFLNDLLGLDLSTGDILALLTRTEGWITGLQLAALSMQGR
                     VDKHEFITAFSGSHHYIIDYLVDEVMARQIEKIQTFLYQTSILDRFSASLCNDVLEIS
                     NSNDVLQALDRASLFLIPLDDEHTWYRYHHLFADFLRQRLGEREPGSIPKLHRRASKW
                     LEKNKFLTEAINHSLAGEDFEQAARIVESLGPEMMMRSEFDQLSSWLDAIPQQMVVAW
                     PWLCIIRAWMCQRWARLKEAEENLQYAERALEDENTPEPVGGKDVIQGQAAAIRALIA
                     LNKAQIPQSMEYANQALAYLPDNHFN"
BASE COUNT          522 a          461 c          428 g          521 t
ORIGIN      
        1 tccacatcat ccgtggaacg gttctcaaaa aattgtgcct ctaagcggag ctgacgcgaa
       61 ctacgtgatt ggggatattt gtcattttag cattgctttc cagaatgata attctcacaa
      121 ttaaaatcgt caagtaatgc ctgcgttaca cattctgaac acaggtatat tatctgatta
      181 tttctctcct tcccctggtt ttcaggctca agacagtagg acaatcgcat ttgggcgatt
      241 tcccaccact gaggagagag attggggtgt tttggtgggg gcagaccccc accaaaacac
      301 cccaaacact ctttttacgg gattgcgcat gcccctcagg gcatatgcaa ttgccctgcc
      361 tcaagacagt ttataacgtg cgcaatgtac aaaaatcgtg taagatatat caattcaata
      421 gacctaactg acaaaatctg gtttcaaccg tggcgattcc gctcctgacc acaaaacttt
      481 atattcccca aacctctcca ggcttggttt cgcgcccgca cctgatttca aggctcaatg
      541 ctggtatggg ggttaagttt gtcctgatat ctgcgccggc aggttttgga aaaagcactc
      601 taatgagcga atgggccagg accatcgata aacccgtcac ctggctctct ttagatgaga
      661 atgacaataa ccttaagcgc tttctttctt atctgattgc tgcactccgg caaattgacg
      721 gatcgattgg ggagagcatt ctagcttcac tagaatcgac agacaaccct ccgattgaac
      781 ttgcaatcac catcttgatc aacgagattg cagagtcagg ggtagagttt tcattggtgc
      841 tcgatgatta tcatctgatt acttcacaga agataaatga agccctaaat ttcttcctcg
      901 accacctacc tccgaacgct cacttgttga taagcacacg agtcgatcca ccattacctc
      961 tatctcgtta ccgtgccagg agtcaaatgg tcgaaatacg cctcgatgat ttgcgcttta
     1021 cagaagcaga agttagatct tttctaaatg atctccttgg attggatttg tccactgggg
     1081 acatactggc gctgcttact cgcacagaag gctggatcac agggttgcaa ttggcggctt
     1141 tatctatgca agggcgcgta gataagcatg agtttattac cgcattttca ggaagccatc
     1201 actatattat cgattaccta gtagacgagg tcatggctcg tcagatagaa aaaatccaaa
     1261 catttctgta ccaaacctcc atcttggatc gtttcagtgc ttctctttgc aacgatgttt
     1321 tagaaatttc caacagtaat gatgtcctgc aagctttaga tagagctagt ttgtttttaa
     1381 ttccattaga tgatgagcat acctggtacc gttatcatca tttattcgcc gattttctgc
     1441 gccagcgttt gggcgaaaga gaaccaggga gcattcccaa attgcatcgc agggcctcca
     1501 aatggctaga aaagaacaaa ttcctcactg aagccatcaa ccattcgttg gcaggagaag
     1561 atttcgagca ggctgcccgg atagtagaaa gtctcggccc cgagatgatg atgcgaagcg
     1621 agtttgacca gttatcgagc tggctagatg caatccccca acaaatggtt gtcgcctggc
     1681 cctggctttg catcattcgg gcttggatgt gtcagagatg ggctcgttta aaagaagcag
     1741 aagaaaactt gcagtacgcc gaacgagctc tagaggatga aaacactcca gaacccgttg
     1801 gtgggaaaga tgtaattcag ggacaggcgg ccgcaattcg cgctctaatt gcccttaata
     1861 aggcacagat ccctcaatcc atggaatatg ctaatcaggc gcttgcatac ctgcctgata
     1921 accactttaa tc
//
LOCUS       DBNHOH010000227         2340 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_910867, whole genome shotgun sequence.
ACCESSION   DBNHOH010000227 DBNHOH010000000
VERSION     DBNHOH010000227.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_910867"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="AC16V7_04010"
     CDS             complement(<1..906)
                     /locus_tag="AC16V7_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR6256316.1"
                     /translation="MKSLLEKLNIKDVNPGACTGSDGWITDPNGKELVSYNPATGEPI
                     AKVVQATPETYETIASTAYNSFLAWREIPAPKRGELVRDLGIALREYKEPLGDLVTIE
                     MGKIRVEGHGEVQEMIDIADFAVGLSRQLYGLSMHSERPQHRMYEQWHPAGVLGLITS
                     FNFPTAVWSWNSTLAAVCGDTVVWKPSSYTPLTAIATQHISNRVMAEHGYSGIFNLVI
                     GSGRDVGELLLNDPRMPVVSFTGSTSIGRHVSEAVARRFGRAILELGGNNGIIVAPDA
                     DLDLATRAILFGAVGTAGQRCTSTRR"
     gene            complement(918..2183)
                     /locus_tag="AC16V7_04015"
     CDS             complement(918..2183)
                     /locus_tag="AC16V7_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256317.1"
                     /translation="MPQDLRQTLRIPHARIDEINSLLLDQNSRVVDDLLEVVSRYGTP
                     EGINQKADEARQFPNLLKRIKETNPKYLADLAWLETQRDLGSFINVADYRRNVLGARA
                     DELHFKDDFAVTLEISAAQYFPWIIAIANRSIENGMLMPGRFIKVRKMREQENDGDLP
                     AIAAAMQIIGASYVETLDTKGTDGSNVHLGGPETITGYFGGVGQPNDYPLKWLDEFLY
                     YYTNYGVKQVLNINPGTVLVGYLLHNLGVDIEFKISVFMGNDNPYAALWTLLGAKLFS
                     RDDGTSPLIGFNWSNSVNNQTMEITAEFRKGLGFEEVVRFEHHITETWKSIVRQPYNR
                     RQELVEIADHVANISAKHEGGDPEIDSKRVHPSDILDYFRDRSEVIASGDWDCLLLNF
                     IDKFDALNHTARALTENGLSFVAAQNLHK"
BASE COUNT          542 a          594 c          591 g          613 t
ORIGIN      
        1 acgacgtgtc gaggtacaac gctgcccggc tgtgccaact gcaccaaaca agatggctcg
       61 tgtggctaag tccaagtctg catcaggggc gacaatgatg ccgttattgc cgcccagttc
      121 aaggatggct cgcccgaaac gcctggcgac agcttccgat acgtggcgcc cgatacttgt
      181 agagccagtg aaagacacaa ctggcatcct tggatcgtta aggagcagct cgcccacgtc
      241 tcttcctgag ccgataacaa ggttaaatat cccgctatag ccatgctcag ccatcacgcg
      301 attacttata tgttgggttg ctatcgctgt taatggagtg taggaagacg gtttccatac
      361 tacggtgtcg ccacacaccg cagcgagggt tgaattccag gaccacacgg cagtcgggaa
      421 gttgaatgaa gtgattaagc caagcacgcc tgccgggtgc cattgctcgt acatgcggtg
      481 ttgcggcctt tcggaatgca tgcttaagcc gtaaagctga cgggataacc cgaccgcgaa
      541 atcggcgata tcgatcattt cctgtacttc accgtgccct tcgacccgga ttttccccat
      601 ttcgatagtc accagatcgc cgagcggttc cttatattcc cgtaacgcga tacctagatc
      661 gcgaaccaac tcaccgcgct ttggcgcggg tatttctcgc caggctaaaa agctgttata
      721 cgctgtagag gcgattgttt cataggtctc aggcgtggct tggaccactt tggctattgg
      781 ctctccagtg gcagggttgt atgacaccaa ctctttgccg ttgggatctg taatccaccc
      841 gtctgagcca gtgcaagcgc cggggtttac atccttaata ttcaattttt ccaataagct
      901 tttcatgtac acctcgttta tttatgcaaa ttctgggcag cgacaaagga aagtccattc
      961 tcggtcagcg cgcgagcggt atggttgaga gcatcaaact tatcgataaa gttgagcaag
     1021 aggcaatccc aatctccgga ggcaatcacc tctgatctgt cgcggaagta gtcaagaata
     1081 tccgaaggat gtacgcgctt gctatcgatt tccgggtcgc cgccttcgtg cttggcagaa
     1141 atattggcga cgtgatcagc gatttcgacc agctcttggc gccgattata tggctggcgt
     1201 acgatactct tccaagtttc tgttatgtgg tgttcgaaac gaacgacttc ctcgaatccc
     1261 aagcctttgc ggaattcagc ggtgatctcc atggtctggt tgttgactga attgctccag
     1321 ttgaagccaa tcagtgggga tgtgccatcg tcgcgtgaga acagcttggc cccaagtaat
     1381 gtccaaagag cagcgtaagg gttgtcgttg cccatgaaga cggatatctt gaattcgatg
     1441 tccacgccca agttatggag caagtatccg acaagtaccg tgcccgggtt gatattcaag
     1501 acctgcttca cgccgtagtt ggtgtaatag tacaggaatt cgtctagcca ttttagggga
     1561 tagtcattag gttgccctac gcctccgaag tagcctgtga tcgtctctgg accccctagg
     1621 tgtacattgg agccatccgt gcctttggtg tccagtgttt ccacgtagct cgcgccgatt
     1681 atctgcatgg cagccgcaat cgcaggtaaa tcgccgtcgt tttcctgctc acgcatttta
     1741 cgcaccttga tgaaacgacc gggcatgagc atgccgtttt cgatggaccg attggcgatt
     1801 gcaatgatcc aagggaaata ttgggctgcg ctgatttcaa gtgtcactgc gaagtcatcc
     1861 ttgaaatgca gctcatctgc tctcgcaccg agcacgttgc ggcgataatc ggcaacatta
     1921 ataaatgagc cgagatctcg ttgagtttcc agccacgcca aatctgccag gtatttagga
     1981 tttgtctctt ttattcgctt taataaattg gggaattgac gggcttcatc ggctttttgg
     2041 ttaatccctt caggagtacc atatcttgat acaacttcca agagatcatc gactacacgg
     2101 ctattctggt caagcagcag cgagttgatc tcgtctatcc gggcatgcgg aattctaagt
     2161 gtttggcgca aatcttgcgg cataatagac ctcctgatca agccttagtt gctaaagtcg
     2221 acacaaaaaa atcatttcag catatgcaca agctcttcat attcttcgtc gggcatgcca
     2281 aaccagtttt cgggctgcgg gaaggtctta gcactgactt cttcgacata ctgtgtaaga
//
LOCUS       DBNHOH010000228         1576 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1486969, whole genome shotgun sequence.
ACCESSION   DBNHOH010000228 DBNHOH010000000
VERSION     DBNHOH010000228.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1486969"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            493..>1576
                     /locus_tag="AC16V7_04020"
     CDS             493..>1576
                     /locus_tag="AC16V7_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6256318.1"
                     /translation="MIERKVLQETIQNALDRSRVVALIGPRQCGKTTMARLFVPSTSI
                     NYFDLEDPLSLTRLDQPMTALQDLRGLVVIDEVQRRPDLFPILRVLVDRTPLPARFLI
                     LGSASPDLLRQPSESLAGRISTITMSGFSLAEVGKEAQNQLWRRGGFPLSFTADTEET
                     SLEWRKDFVRTFLERDIPQFGFNIPSTSLFRFWSLLAHYHGQIWNNAEAARTLNFGEA
                     TARRYLDLLQDLFMVRLLQPWYANLSKRQVKTPKIYIRDPGLLHYLLGIRSEKDLLLH
                     PRSGASWEGFAIEETVKAVMPDEVYFWATHSGAELDLLLIKNGQRIGAEYKRVDAPRL
                     TPSMRTACKDLELVRLLVIYPGLKPYP"
BASE COUNT          358 a          401 c          404 g          413 t
ORIGIN      
        1 cagccaggat ttgacctggt ttgtcgctat tttggggctc aaaattcaac ctttaccttt
       61 cggattttgg ttaaagagcc aaagtccagc ccaaacactc tttttacggg attgcgcatg
      121 cccctcaggg catatgcaat tgccctggtg gatgccccct ggagttgatg ggattaccat
      181 cacctagatg tctacttggt gactcagctg ctgcgtgccc aacacgacgc aagcatcccg
      241 tcttacatcg tatcgaagtt ttggtgaagc aggcgctaga ggcgcagagt ggtctgtctt
      301 atgctctctc gaactggcag ctaggggttt cggatgtttt ggcatattta ccaagaatag
      361 ccctatgtat ctgtggaaga gatgctccac gcctacatgg tcatattgac aacaacccat
      421 ctcaagaata cagaacatca ttaccatgaa aatccgccat cgaatggcgg attttcatgg
      481 tatcataagt ccatgattga aagaaaggtt cttcaagaga ccatccaaaa cgcactcgat
      541 cgcagtcggg tggttgcgtt gatcggtcca cgccagtgtg ggaagacaac gatggcgcgt
      601 ctatttgtcc cttccacctc aatcaactat tttgatctgg aagacccatt aagcctcact
      661 cgcttggatc aacccatgac ggcgttgcag gatctacgcg gcctggttgt gattgatgaa
      721 gtccaacgcc gcccggatct attcccaata ctgcgggtat tggtagaccg cactccactg
      781 ccggcgcgtt ttctgatact cggtagcgct tcaccagacc tgctacgcca gccgtcggag
      841 tcgctggctg ggcgtatttc gaccatcacc atgagcggct tcagcctggc tgaagtgggt
      901 aaggaggcgc aaaatcagct atggaggcgg ggtggctttc cgctgtcatt cacggctgac
      961 accgaagaaa ctagccttga atggcgcaag gattttgtac gtacttttct tgaacgggat
     1021 atcccgcagt ttggtttcaa tatcccctcc acgagcttgt ttcgtttttg gagtctgctg
     1081 gctcattacc atggacagat atggaataac gccgaagcag cgcgcacatt gaattttggt
     1141 gaagccacag cccgccgcta tctcgatctg cttcaggatt tgttcatggt gcgcttgtta
     1201 caaccctggt atgccaatct tagtaagcgc caggtaaaga cacccaaaat ctatattcga
     1261 gaccctgggt tgctgcacta cttgctgggt atccgcagtg agaaagactt gcttttgcac
     1321 ccgcgcagcg gcgcttcctg ggaaggtttc gctatagagg aaaccgtcaa agcagttatg
     1381 ccagatgagg tttatttttg ggcgactcat tcgggagcag agcttgatct gctgctcatc
     1441 aagaatgggc agcgcattgg cgcagagtat aaacgtgtgg atgcgccacg gctgacgcct
     1501 tcgatgcgca ctgcctgcaa agatttggaa cttgtccggt tgttggtcat ttaccctgga
     1561 cttaagcctt atcctc
//
LOCUS       DBNHOH010000229         2248 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1039156, whole genome shotgun sequence.
ACCESSION   DBNHOH010000229 DBNHOH010000000
VERSION     DBNHOH010000229.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1039156"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1267)
                     /locus_tag="AC16V7_04025"
     CDS             complement(<1..1267)
                     /locus_tag="AC16V7_04025"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HIR6256319.1"
                     /translation="MAEPNVLPRSEIPEKYTWNSTSVFSTKEAWEAALKELPELTARV
                     TKYQNHLGDNPSMLVEALQAFEKLYRQSGMVYVYATMSQAVDTTNQEANRIRSRAQSA
                     FAQVFAALAFIDPELISIGQDKLRKWIEDEPRLRSYGHYLENLFRRQAHVRSPEVENL
                     LGMLRDPMGNIYNTANMLANADIQFSPAYTSDGGEVQVSNSTYDSILSSPDREARRTA
                     YESYTDSYLSLKNTFASNLITSVKSNIFMMRGRRHPSTLEASLFESNIPTSVFHNLID
                     TYRKNLPTWHRYWAIRRKALGVDELQPYDIWAPLTQKHPKLTYEETVEWICRGLAPMG
                     KDYVDTIRRGCLEDRWVDVFPNQGKMDGAFSSGWPGTFPFILMSFDGTIFSLSTLAHE
                     LGHSMHSHLTFEHQPVIYSDYSVFLAEVAS"
     gene            complement(1388..>2248)
                     /locus_tag="AC16V7_04030"
     CDS             complement(1388..>2248)
                     /locus_tag="AC16V7_04030"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6256320.1"
                     /translation="FRRVAVLLDGSATAAQALPMAKRVCQATDAEMILLSSVKAVGDP
                     DYENIFNERKAYLRQQCYELEQQGYRVSYTVRPGHIADATSALVKEQYIDLIVTSTSG
                     KSGTKHWQRGGVSRKLMALLNIPVLLVKVSEMDILMPSFKRIVLSLDGSEFSERALPY
                     ASVFAELFDSELILLSVPEVPEVKDYRAAAGVIDTIREKAYANMHEYLNETANSLLQT
                     NGKINVRTMVAGSLPARTIVNVSEEENADLIMLTSQGRGGLDLLMTGSVALNVVEDTD
                     LPVFMVPIIS"
BASE COUNT          561 a          572 c          519 g          596 t
ORIGIN      
        1 ttgatgctac ctctgctagg aacacagagt aatcgctgta aatgacgggc tggtgctcaa
       61 aagtaaggtg ggaatgcatg gaatgcccca gctcatgtgc cagcgtgctc aggctaaata
      121 tcgtgccatc gaagctcatc aatataaagg ggaaagtccc cggccaaccc gaagaaaatg
      181 ctccatccat tttcccctga ttagggaaca cgtccaccca tcgatcttcc aggcaacctc
      241 tgcggatggt atccacgtag tcttttccca tcggcgccaa gcctctgcaa atccattcga
      301 ccgtttcttc ataggttagc ttagggtgct tttgagtcag gggcgcccag atgtcatatg
      361 gttgaagctc atccaccccc aacgctttgc ggcggattgc ccaatagcgg tgccaggtcg
      421 ggagattttt gcgataagta tcaatcagat tgtggaaaac cgacgttggt atattgctct
      481 cgaagagtga tgcctccagt gtcgagggat gtcgtcgacc acgcatcatg aaaatattgg
      541 acttcaccga ggttataagg ttgcttgcaa aggtgttctt tagtgataaa tagctatctg
      601 tatagctctc gtaggcggtg cgccgcgcct cccggtctgg gctggacaga atgctatcat
      661 acgtactatt ggatacttgc acttctccac catcactagt ataagccggg gagaactgta
      721 tatcagcgtt ggcaagcata ttggctgtat tgtagatatt acccatagga tcgcgtagca
      781 tgccaagtag attttccact tcaggcgaac gcacatgagc ctggcggcgg aaaagatttt
      841 ccaggtagtg accatagcta cgcaggcgcg gctcgtcctc gatccatttc cggagtttat
      901 cctgcccgat ggatatcagc tcaggatcaa tgaacgccaa agcggcaaaa acttgtgcga
      961 aggctgactg agccctgctt cggatccggt tggcttcctg gttagtggta tccactgcct
     1021 gagacatcgt ggcgtaaacg taaaccatcc cagattggcg ataaagtttc tcgaaagcct
     1081 gcagcgcttc gaccagcata gatgggttgt cccccaaatg gttctggtat tttgtgacac
     1141 gcgccgtcaa ctcgggcagt tctttcaatg ctgcttccca agcttctttt gtcgagaaca
     1201 cgctagttga gttccaggtg tacttctcag gtatctcact gcgaggtaaa acattaggtt
     1261 cagccattgg tttctccaag tttataacaa agatttttta ttcttcatct ccgtcaacac
     1321 ctgactttgt cctgctttga aggcaattgc cctaaaagat gcgtttgctc attaaagcat
     1381 tcatcgctca ggagattatt ggcaccataa agacaggcag gtcagtatcc tcaacaacgt
     1441 ttaacgctac gctaccagtc atcaacaggt ctaagccccc gcgcccttgc gaggtcaaca
     1501 taattaaatc agcgttttct tcttcgctca cgtttacaat cgtacgtgct ggcagcgagc
     1561 cggcgaccat ggttcgcaca ttaatcttgc cgtttgtctg caataagcta ttggcggttt
     1621 cgttcaaata ttcatgcata ttggcataag ctttctctcg aatagtatca atgacccctg
     1681 ctgcagcgcg atagtccttt acctctggta cttctggtac agacaacaag atgagctcgc
     1741 tgtcaaataa ctctgcaaac acactcgcat atggcagtgc gcgttcagaa aactcggaac
     1801 catccagcga gagaacgatt cgcttaaaag aaggcattaa gatatccatc tcagatacct
     1861 tcaccagtaa aacggggata ttgagaagcg ccattaattt gcgggaaacc ccaccccttt
     1921 gccagtgttt agtgccagat ttcccactcg ttgaggtgac aatcaaatcg atatattgct
     1981 cttttaccag tgcgctggtc gcatcagcaa tatgtccagg acgtacagtg tagctaaccc
     2041 tatatccttg ctgctccaat tcataacatt gttgtctcag ataggctttc cgctcgttaa
     2101 atatattttc ataatcggga tcacccacag cttttacaga agacagcagg atcatttccg
     2161 catcggttgc ctggcatact cttttagcca ttggcagggc ttgagcagcg gtcgctgaac
     2221 catccagcaa gacagcaacc cgccggaa
//
LOCUS       DBNHOH010000230         9740 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1167009, whole genome shotgun sequence.
ACCESSION   DBNHOH010000230 DBNHOH010000000
VERSION     DBNHOH010000230.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9740)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9740)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9740
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1167009"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="AC16V7_04035"
     CDS             <1..932
                     /locus_tag="AC16V7_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256321.1"
                     /translation="GIPVRSHRPSRSLRDEPATHCLLTLAALAHPAWDFLPSKFDIAY
                     ALVQAIQGMDLVRARLLSEIVYRARAGAPVLSPFDRIKADVQERITYLLGNRYEDLRL
                     WLAEYAQSPEAELDHFLSRLFGELLSQPGFGFHVNYAAGEVTANLIESVQKFRWAVGD
                     VLADEGIPLGKEYLMMVQDGVIAAQYIRSWQILPGDAVMVAPAYTFLMSNRPVEVQFW
                     LDVGSRGWFERLYQPLTHPYVLSRGWGQGQIWTDEHEFHASQDSLRRLVLGLVRRCRR
                     GIYLGLSELSEQGYEERGMLLQVIQRVLREGRA"
     gene            938..3178
                     /locus_tag="AC16V7_04040"
     CDS             938..3178
                     /locus_tag="AC16V7_04040"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR6256322.1"
                     /translation="MEFIPRPKQQEVLDYTGGYMGVSAVPGSGKTWTLSLLAAQIVAS
                     GELDSDQEVLVVTLVNSAVDNFYKRVSDFIQAAGLLPHLGYRVRTLHGLAHDIVRERP
                     ELVGLADNFQIIDERQVDSIRSEVAEAWLRANPNALDGYLNPELDDERLAWVRREPLP
                     NLVSEIALSFIRYAKDLQLTPERLRLQLESLPVPLSLAEMGHAIYVDYQRALSYRGAV
                     DFDDLIRLALEALRLDDKLLERMRYRWPYILEDEAQDSSRLQEEILKTLAGPGGNWVR
                     VGDPNQAIYETFTTANPKYLRAFIKSRGVLGRELPNSGRSTQTIIDLANHLVKWTQRQ
                     HPVRQAREALGGPPFIEPVPEGDEGANPPDDLSKLHLVARKYSPVDEISAVVDSLQRW
                     LPDHPDDTVAVLVPRNQRGFELVDVLRRRGIEVVDSLLRSTASTRFSARVLTQLLSYL
                     SDPGSPSRLALLYKAWQRGEQAGARRERVSELLRKCGQVEDFIWPNPGQDWLEELDLE
                     DTEPQVYTQLLEFRQLVRRWQEAILLPVDQMILTASQDLFTEPSQLALAHKLAALLRQ
                     AGQSHPSWRLPELTEELAVIARNERRFLGFSDDDTGFDPGQYRGKVVVATMHKAKGLE
                     WDRVYLMSVNTYDFPSGLQGDRYISEKWFLRDNLNLEAETLAQLDALLSTDEYTWYQE
                     SEATHRARLDYVRERLRLFYVGITRAKKELVVTWNTGRRGELQPSTPLLALMDYVSSQ
                     ETSNDL"
     gene            3215..4096
                     /locus_tag="AC16V7_04045"
     CDS             3215..4096
                     /locus_tag="AC16V7_04045"
                     /inference="COORDINATES: protein motif:HMM:NF024117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR6256323.1"
                     /translation="MKLPDGFIFSQSSLQDFVDCRRRFQLRYINRLAWPALETEPALE
                     AERYLQQGARFHRMVQQHLLGVPAERLSNMITDDDLSRWWHNYLSFVQALRQQSAAPT
                     EGVSEHPLFSAARGYPEISLSATLAGFRLEAKFDLVLLLPDGGTRIYDWKTSRRRPER
                     RWLANRLQTRLYPYLLTRAGAVLNGGKDLLPEKVGMIYWFADHPGQPERIAYSAQAFR
                     DDEAYLTDLVATIQRLGDDDFPLTTDLRRCAFCSYRSLCDRGARAGDLDEMQGEADIT
                     DEMALNLDFEQIGEIEF"
     gene            complement(4127..5353)
                     /locus_tag="AC16V7_04050"
     CDS             complement(4127..5353)
                     /locus_tag="AC16V7_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6256324.1"
                     /translation="MNIIQSFYEALESVSSNKMRSGLTILGIVIGVAAVIAMVSVGRG
                     AQNTITGSIQGIGTNLLFVFRGGSEDVRNPKPITLGDGDAIADPFQAPSVADVAPMLQ
                     GDGIVSYAGENHFTSIIGVTPSYGPVRNTSVSEGEFIREEHILGQASVVLLGPEVAKK
                     LFNRTEGLVDETVRIEGQPFRVLGVLESKGGSAFSNQDDRILVPFSTAQTRLLRRATR
                     DRVDLLMVQAVSAQAVPQASEEVAQILRTRHRTEVGADDFTVLTQQDFLDTAQTITGV
                     LTVFLGGIAAISLLVGGIGIMNIMLVSVTERTREIGLRKALGARKIDILVQFLTESSV
                     LSLIGGIVGIMLGWLISFIVGRIAAANEADITPAIGVDTILLATLFSTAVGLFFGLYP
                     ANRAANLEPVEALRYE"
     gene            complement(5350..6048)
                     /locus_tag="AC16V7_04055"
     CDS             complement(5350..6048)
                     /locus_tag="AC16V7_04055"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6256325.1"
                     /translation="MDNWVIEAENLTKVYKMGEVEVHALRGLSLRISRGEVAAIMGPS
                     GSGKSTLMNMIGCLDQPTSGEYILDGERVSNLKDDQLATIRNRKVGFVFQSFNLLPRA
                     TALANVELPLRYAGVGRGRKDRAREALELVGLADRIHHRPNELSGGQQQRVAIARALV
                     NNPAIILADEPTGNLDSKSGEEIMQLLLGLNQEHGTTLVIVTHDPVVASRTPRIIQIM
                     DGVVVEQDTGNRNQ"
     gene            complement(6051..7601)
                     /locus_tag="AC16V7_04060"
     CDS             complement(6051..7601)
                     /locus_tag="AC16V7_04060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6256326.1"
                     /translation="MRKWWIGGIAVLILVSAGLAISFLRSQRQAAALSGLLTEPAQRG
                     DLTATVGATGVVRANQTALLTWQTTGTVGEVNVGVGDQVAKEQVLAVLDQTSLPQSII
                     LAQAELVTAQRALEDLLNSQIQQAQALQAVDDARQALEDAGNPELAQAKAQQAIADAQ
                     KAVENAERLLRAAKSPASQSFIDEAQARVVLTRDILEQAQERFEPYQNKPQDNLIRAR
                     LQTELAAAQQQYDAAVRILNSLQGTSNPIDQAVAQADLETAQAQLVEAQREWERVKDG
                     TSPAALALLEAQLADAEREFERVKNGPDPDDIAAAQARVAAAQAAINLASIHAPFAGM
                     ITQSESKTGDQTAPGSVAFRLDDLSRILVDVQISEVDINRIQIGQDVNLVFDAIPNQE
                     YHGTVTEVALVGTSSQDVVDFIVTLELVDADQAVKPGMTAAVNIVVNQLKDVLLVPNR
                     AVRILEGKRVVYLLRGEAPDPVEIQLGASSEASSEVIGGDLKEGDPIVLNPPTVFEQN
                     GPPPFVRR"
     gene            complement(7638..9209)
                     /locus_tag="AC16V7_04065"
     CDS             complement(7638..9209)
                     /locus_tag="AC16V7_04065"
                     /inference="COORDINATES: protein motif:HMM:NF052757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR6256327.1"
                     /translation="MRKAFIILVIFSVLVFAGLAISRASSLRALASRTDYETLPVRRG
                     DLQITVTAAGVLRADQSAVLRWQTSGSIESVNVKKGDPVSMGQILASLEQTSLPRQLI
                     LAQVDFIEAQRKLDDLLYSQTQPAQAQKAVEDAERALEDALESNLVQSNAMAAFVQAQ
                     KAVDTAQRNLDILLTPPSQDGIDAARATLLQWEHSLELIRLDIERVQRKLQKDEDAYS
                     PGESRDSYEAQLESLLQKVVRYQRLYEDAVARYQRLQMPPDPNDLAVAEAKLKLAQAQ
                     LRQAQSDWERVKDGPNPADIAVLQARLDDSRRTAEHLKDGPDPDEVTAARARLAAAQA
                     ALDLAHIKAPFDGVITEAAAQPGDQVLPGDVAFRLDDLSPLWIELDVSEIEVNRIREG
                     QPVIITLDSSPGRQYNGEVVDVSAVGKISSGVASFRVKAEIRDADRASRPTMTASASI
                     IVAEMKDVLIVPASAVRFIDGQRVVYVLRQGQPTPVHVTLGSFSSNEIQVLEGDLQPG
                     EAVILNPDDREQGSS"
BASE COUNT         1962 a         2826 c         2822 g         2130 t
ORIGIN      
        1 ggggcatacc ggtgcgctcc caccgccctt cgcgctcgct gcgtgacgaa ccggcgaccc
       61 actgcttgct caccctggcg gctctggccc atcccgcctg ggacttcctg ccctccaagt
      121 tcgacattgc gtatgcgctg gtgcaagcta tccagggcat ggacctggtg cgcgcccgcc
      181 tgttgagcga gatcgtctac cgcgcccgcg ccggtgcgcc tgtcctctcc ccctttgacc
      241 gtatcaaggc cgatgtccag gagcgcatca cttacttgct gggaaaccgc tacgaagatt
      301 tgcggctgtg gctggcggag tacgcccaaa gccccgaggc tgagctggat cactttctca
      361 gccgcttgtt tggcgagttg ctctcgcagc ctggcttcgg cttccacgtg aattacgccg
      421 ccggggaggt caccgccaac ctgatcgagt cggtgcagaa gttccgctgg gcggtgggag
      481 atgttctggc agacgagggc atccccttag ggaaggaata cctgatgatg gtgcaggatg
      541 gggtcatcgc agcccaatat atccgtagct ggcagatact gccaggcgat gccgtgatgg
      601 tagccccagc ctataccttc ctgatgagca accgcccggt cgaggtgcag ttctggctgg
      661 atgtgggcag ccgcggctgg ttcgagcgac tgtaccagcc gctcacccac ccctacgtgc
      721 tcagccgggg ctgggggcag gggcaaatct ggacggatga acacgagttc catgccagcc
      781 aggattccct gcgccgcctg gtgttgggtc tggtgcgccg ctgccgccgg ggtatctatc
      841 tggggctgag cgagctgagc gagcagggct acgaggagcg cggcatgctg ctgcaagtga
      901 tacagcgtgt tctgcgagag ggcagggcgt agaaaacatg gagttcattc cacgccccaa
      961 gcagcaagaa gtactcgact acaccggcgg ctacatgggt gtctctgccg tgccgggcag
     1021 cggcaagacc tggacgctct ccctgctggc agctcagatc gttgccagcg gcgagctgga
     1081 cagcgaccag gaagtcttgg tggttacgct ggtcaattcc gcggtggata atttctataa
     1141 gcgtgtcagc gacttcatcc aggccgccgg gcttcttccg cacctgggct accgcgtgcg
     1201 cacattgcat ggtctggcgc acgatatcgt ccgcgagcgc cctgagctgg tggggctggc
     1261 ggataacttc cagatcattg acgagcgcca ggtggacagc atccgcagtg aggtggctga
     1321 ggcatggctg cgcgccaacc ccaatgcact ggatggctac ctgaaccctg agctggatga
     1381 cgaacgtttg gcctgggtgc gccgagagcc gctccctaac ctggtcagcg aaattgccct
     1441 gagcttcatc cgctatgcaa aagaccttca gcttaccccc gagcggctga gattgcaatt
     1501 ggagagcctg cccgtgccgc tcagcctggc tgagatgggt cacgccatct atgttgacta
     1561 ccagcgcgcc ttgtcctacc gcggcgcggt ggactttgat gacctcatcc gcctggccct
     1621 ggaggcgttg cgtctggacg ataagttgct ggagcggatg cgctatcgct ggccgtatat
     1681 cctggaggac gaagcccaag acagcagccg tctgcaggag gagattctca agaccctggc
     1741 tggcccaggc ggcaactggg tgcgcgtggg cgacccgaac caggccatct acgagacttt
     1801 taccaccgcc aacccgaaat atctgcgcgc tttcatcaag agtcgcggcg ttttggggcg
     1861 ggagctgccc aactcaggcc gctccacaca gacaataatc gacctcgcaa atcacctggt
     1921 gaagtggacc cagcgccagc acccagttcg ccaggctcgc gaggccttgg gaggaccgcc
     1981 cttcatcgag ccggtgcccg agggcgatga aggcgccaac cccccggatg atctatctaa
     2041 gttgcacttg gtggcgcgca aatacagccc tgtagacgaa atctcggcgg tagtcgattc
     2101 gctgcagcgc tggctgccag atcacccaga tgacacggta gccgtgctgg tgccgcgcaa
     2161 ccagcgcggc ttcgagttgg tggacgtttt gcgccgccgg ggcatcgagg tagtggacag
     2221 cttgttgcgc agcacggcct cgacccgctt ctctgccagg gtgctcactc aactgctcag
     2281 ctacctctcc gatccgggct cgccatctcg cctggcccta ttgtataaag cctggcagcg
     2341 cggtgagcaa gcaggtgccc gccgtgagcg cgtctccgag ctgctgcgta agtgtggaca
     2401 ggtggaggac tttatctggc ctaacccggg gcaagattgg ttggaagagc tggatctgga
     2461 ggacactgaa ccacaggtct atactcagct cctggagttt cgccaattgg tccgccgctg
     2521 gcaggaagcc atcctcctgc ctgtagatca gatgatcctc accgcctcgc aagacctgtt
     2581 taccgagccg tcacaattgg cattggcaca caagctggcg gcgctgctgc gccaggcagg
     2641 ccaatcgcac ccctcctggc gcctgcccga gctgactgaa gagctggcgg tcatcgctcg
     2701 caatgagcgc cgtttcctgg gtttcagcga tgacgacacc ggcttcgacc cgggccaata
     2761 tcggggcaag gtggtggtcg ccaccatgca caaggccaag ggcttggaat gggaccgtgt
     2821 ctacctgatg tcggtcaaca cctacgattt cccctccggc ctccagggag atcgctacat
     2881 ctcagagaag tggtttctgc gcgacaacct gaacttagaa gctgagacgt tggcgcagct
     2941 ggatgcgttg ctctcgaccg atgagtacac ttggtaccag gagagcgagg ccacccaccg
     3001 cgcccgcctg gattatgtgc gtgaacggct gcgcttgttc tatgtgggca tcacgcgcgc
     3061 caaaaaagag ctagtggtca catggaacac tggcaggaga ggcgagctgc aaccatccac
     3121 gccgctatta gccttgatgg actacgtgag ctcccaggaa acatccaatg atctgtgatt
     3181 atctcgatcg gtcaagatgt ctggagactg acccgtgaaa ctgcccgatg gttttatctt
     3241 tagccagagt agcttgcaag attttgtgga ctgccgtcgc cgctttcagt tgcgctacat
     3301 taaccgcctg gcatggccag ccctagagac ggagccagcc cttgaggctg aacgctacct
     3361 gcagcagggt gcgcgtttcc atcgcatggt gcagcagcat ctcttgggtg tgccagcgga
     3421 gcgtctatct aacatgatca ctgacgatga cctgagccgc tggtggcata actacctgag
     3481 cttcgtccag gcgttgcgcc agcagagtgc ggctcctaca gagggggttt ctgagcatcc
     3541 ccttttcagt gcggcgcgag gctatccgga gatcagtctg tctgcgacac tagccggttt
     3601 tcgcctggaa gccaagtttg acctggtgct attgctgccg gatgggggca ctcgaattta
     3661 cgattggaag acgagcaggc gcaggcctga acggcgttgg ctggctaacc gtctgcaaac
     3721 gcgcctgtac ccctacctgc ttacccgtgc tggtgctgtt ctcaatgggg gaaaggatct
     3781 actgcccgag aaggttggga tgatttactg gttcgccgat cacccgggac aaccggagcg
     3841 tattgcctat agcgcacagg ctttccggga cgatgaggca tacctgaccg acctggtcgc
     3901 aaccattcag cgtctgggcg acgacgattt ccccctgacc accgatctgc gccgctgcgc
     3961 cttttgcagc tatcgctctc tttgcgatcg cggcgcacgc gcgggagatt tggatgagat
     4021 gcagggggag gcggatatca ccgatgaaat ggctctcaac ctggattttg agcaaatcgg
     4081 cgaaatcgag ttttaaccaa taactactac catcccaaac tgcacatcac tcgtatcgca
     4141 gcgcctctac cggttccagg ttggcggcgc ggttggctgg gtatagtcca aagaataagc
     4201 ccaccgcggt tgagaagagg gttgccagca ggatggtatc cacgccgatg gcgggggtga
     4261 tgtcggcttc atttgccgcg gcgatccgcc ctacgatgaa agagatcaac catccgagca
     4321 tgatgcccac gatgccccca atcaggctga gtaccgagga ttcggtgaga aactgcacca
     4381 aaatatcgat cttgcgcgct cccagggctt tgcgcagacc aatctcgcgc gtgcgctcgg
     4441 tgaccgagac cagcatgatg ttcataatgc cgatcccgcc aaccagcagc gagatggcag
     4501 cgatgccgcc caggaaaacg gttagtacgc cagtgatggt ctgggcagta tccaggaaat
     4561 cctgttgggt aagaacggtg aagtcgtcgg cgccaacctc cgtgcggtgg cgcgtgcgca
     4621 ggatttgagc gacctcctca gaagcctggg ggaccgcctg ggcgctaacc gcctgtacca
     4681 tgagcaggtc cacccggtcg cgggtggcgc gtcgcaacag acgggtctgg gcggtggaga
     4741 acggaacgag aatccggtca tcctggttgg agaaggctga gccgcctttt gactccagca
     4801 cgccgagcac ccggaaaggt tggccttcta tacgcactgt ctcgtctacc agcccctcgg
     4861 tgcggttgaa taatttcttc gccacctctg ggccgagaag aaccaccgag gcctgaccca
     4921 agatatgctc ctctcgaata aactcaccct ctgagacaga ggtattgcgc acgggaccgt
     4981 aggatggtgt cactccaatg atggaggtga agtgattttc gccggcatac gagacgatgc
     5041 cgtcgccctg cagcatggga gctacatctg ccacggaggg tgcctggaac gggtcggcga
     5101 tggcgtctcc atctcctaag gtgatgggct taggattgcg cacatcctcc gagccaccgc
     5161 ggaagacaaa caacaggttg gtgccgatgc cttggattga gccggtgatg gtgttttggg
     5221 cgcctcgccc aacagatacc atagcgatca ctgccgccac gccgataacg atccccagga
     5281 tggtcagccc ggagcgcatc ttgtttgatg acacgctctc cagggcttcg tagaaagatt
     5341 ggatgatgtt cattggtttc ggttccctgt atcctgctcc acaacgaccc catccataat
     5401 ttggataatg cgtggcgtgc gtgaggctac cactgggtcg tgggtgacaa tcaccagcgt
     5461 ggtgccatgt tcctggttaa gtcccaataa caattgcatg atctcctcgc ccgacttgct
     5521 gtcgaggtta ccggtgggct catccgccag gatgatggcg gggttgttta ccagggcgcg
     5581 ggcgatggcg acgcgctgtt gctgcccacc cgaaagctcg ttggggcgat ggtggatgcg
     5641 atcggccagc cccaccagct ctagagcttc acgagcacga tccttgcgcc cgcgccctac
     5701 tcctgcgtac cgcagaggca gctctacatt ggctaatgcc gtggcacgcg gcagcaggtt
     5761 gaagctctgg aagacaaagc ccactttgcg gttgcggata gtggcgagct ggtcgtcctt
     5821 caagttcgag acgcgttccc catccaggat atactcgccg gaggtgggct ggtctaggca
     5881 gccgatcatg ttcatcaggg tggatttgcc cgagcctgat ggtcccataa tggcagccac
     5941 ttcaccgcgg gaaatcctga gggacaaccc gcgaagggca tgcacctcta cctcgcccat
     6001 cttatatact ttggtcagat tctcagcttc gatgacccaa ttgtccatcg ttacctccgt
     6061 acaaatggcg gagggccgtt ttgctcaaag actgtcggtg gattgagcac gattgggtcg
     6121 ccctccttca aatcgccgcc aatcacctca ctcgatgcct ccgaggatgc cccaagctgg
     6181 atttctaccg gatctggggc ctccccgcgc agcaggtaaa cgaccctctt tccctccaaa
     6241 atacgtactg cacggttggg gaccagaagc acatctttca actggttaac cacgatgttg
     6301 accgcggcgg tcatgcccgg cttaactgct tggtcagcat caaccagttc cagggttact
     6361 ataaaatcga ccacatcctg gctggaagta cccacaagag caacctcagt cactgtgccg
     6421 tgatattcct ggtttgggat ggcgtcgaag acaaggttca catcttgccc tatttgaatg
     6481 cggttgatat ccacttcaga gatttgcaca tccaccagta tcctggaaag gtcatctagg
     6541 cggaatgcta cgcttcctgg ggcggtttga tccccggtct tgctctctga ctgtgtgatc
     6601 atccccgcaa aaggagcatg gatagatgcc aggtttatgg cggcctgggc ggcagcgacg
     6661 cgcgcctgag cggcggcgat gtcatctggg tcagggccgt ttttcacgcg ctcaaattcg
     6721 cgttcggcgt ctgccagttg tgcttccagc aaagcgagcg cggctgggct ggtgccatcc
     6781 ttaacgcgtt cccactcgcg ctgagcttcg actaattgag cctgggcggt ctctaagtcg
     6841 gcttgcgcca cggcctggtc aatggggttg gacgtgccct gtaggctgtt caatatcctt
     6901 acggcggcat cgtattgctg ctgggcggcg gctaattcgg tctgcaggcg cgcacgtatc
     6961 agattatcct gtggcttatt ctgataaggc tcaaaacgct cctgcgcttg ctccaggata
     7021 tctcgtgtaa gaaccacccg agcctgcgcc tcgtcaatga agctctggct ggcaggcgat
     7081 ttggctgccc ttaataaccg ttcggcattc tcgacagctt tctgcgcgtc agctatggct
     7141 tgctgtgctt ttgcctgtgc taattctgga ttgcctgcat cctctaaggc ttggcgggcg
     7201 tcatcgacgg cttggagggc ctgtgcctgc tggatttgcg agttgagtag gtcttcgagc
     7261 gcgcgctggg cggtaaccag ttcagcttga gccaggatga ttgattgggg caaagatgtc
     7321 tgatcgagca ctgccagtac ctgctccttt gcgacttgat cgcctacccc gacattcacc
     7381 tcgcccactg tgcccgtggt ctgccaggtt aataatgcgg tttgattagc gcgtaccaca
     7441 ccggtagcgc ctactgtggc agttaaatcg ccgcgctggg ctggctcggt tagcagtcca
     7501 gataacgcgg cagcctgtcg ttgactgcgt aaaaagctta ttgccaaacc tgcactgact
     7561 aggatcaaaa cggcgatacc gccgatccac catttcctca tacttcctcc aacaccattt
     7621 tacttagctc aagaatatca cgagctgcct tgttctcggt catcaggatt caatataacc
     7681 gcttctcctg gttgcagatc gccttccagc acctgtatct cattagatga aaaagaaccc
     7741 agcgtgacat gcactggggt aggctgccct tgccgcagca cgtacaccac ccgctgtcca
     7801 tcgataaagc gcacggcgct tgctggcacg atcagcacat ctttcatctc agcgacgatg
     7861 atactggcgg aagcggtcat ggttgggcgg cttgcgcggt cagcgtcgcg aatttcagcc
     7921 ttgacgcgaa atgaagctac tccactggag atcttgccga ctgcagaaac atcgaccacc
     7981 tcgccattat attggcgtcc tggcgatgag tccagcgtaa tgattacagg ctggccttca
     8041 cggatacggt tgacctcgat ctccgagacg tccaattcta tccataacgg tgacaggtcg
     8101 tccagccgga aagctacatc cccagggaga acctggtctc caggctgtgc ggcagcctcg
     8161 gtgatcacgc cgtcgaaggg agctttgata tgtgccaggt caagcgcggc ctgggctgca
     8221 gccagccgtg ctcgtgctgc agtaacttca tctggatcgg ggccatcctt caggtgctcg
     8281 gctgtgcgac gggaatcgtc cagcctggcc tggagcaccg ctatatccgc cgggttgggg
     8341 ccatctttca cgcgctccca gtcactttga gcctgtctta actgcgcctg ggcgagcttc
     8401 aatttggctt ctgccaccgc caggtcattg gggtccggcg gcatttggag tcgttggtac
     8461 cttgctactg catcctcata taaccgctgg tacctgacaa ccttttgcag caagctttcg
     8521 agttgtgctt catatgaatc gcggctttcc ccgggtgaat aggcatcctc atccttctgc
     8581 aacttgcgct gtacccgctc gatatccagg cggataagtt ccaggctgtg ctcccactgg
     8641 agcagcgtgg cgcgggcagc atcgataccg tcttgcgagg ggggagttag caatatatcc
     8701 aggtttcgtt gggctgtatc gacggctttc tgcgcctgga cgaaggcagc catagcattt
     8761 gactggacga gattggattc gagagcatct tccaacgctc gctcagcatc ctctaccgcc
     8821 ttctgtgcct gcgcgggctg ggtttgagaa tacaaaagat catccagctt gcgctgcgct
     8881 tcgatgaaat ccacttgggc caggatgagc tggcgcggca gggaggtctg ctccaaactc
     8941 gccaggatct gacccatgga aaccggatca ccttttttga catttacgct ttcaatcgaa
     9001 cccgaagtct gccatcttaa aacggctgac tggtcagcgc gcaatacccc tgcggctgtt
     9061 accgttatct gtagatcacc ccggcggact ggcagggtct catagtctgt ccggctggcc
     9121 agggcgcgca gactggatgc acgggagatg gcaagtccag caaacaccaa tacggaaaaa
     9181 atgacgagaa taataaacgc ttttctcatt gaagtgctat ctaaagccaa tctccttttc
     9241 cgccctcacc tcaagacctt gctttatcga gaaagaaggg tacgcgacct agcatataat
     9301 ggatcgacat aaggtagggc ggccgctaaa ttataacttg ttggcgggtc aagaagaaga
     9361 atattaaata tttctcaccc tccatcttgg gcggtgcacc atccaagcgg gtgataagcg
     9421 tcatgatccc tgtaaatccc agtatattgg gtaagtgaca accgtcatca aaagaagtga
     9481 catctgagac tgaaattaaa cctattgtgt cgttattatc ttagtactta cgcaattgaa
     9541 ggaaaaggtg gattggcagg tgtagcctgc ccaaccctcc attacatctc caactgcgta
     9601 acttctgtat cttaagcatc gaaagagatc gaacgcaagg aggacatcat catgcaggca
     9661 atcccgagcg attttttagc acttgatcga aaaacacgcg ctcgtatccc gtttctggat
     9721 gtagacctac gcagtcccca
//
LOCUS       DBNHOH010000231         3520 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1167044, whole genome shotgun sequence.
ACCESSION   DBNHOH010000231 DBNHOH010000000
VERSION     DBNHOH010000231.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1167044"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..288
                     /locus_tag="AC16V7_04070"
     CDS             73..288
                     /locus_tag="AC16V7_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256328.1"
                     /translation="MYEPNLFAEKYQLALETALKEKPQGGVCGFELEWNLLDDQFRPL
                     LTVGSGPSRQSFVDYLRAECLPSWLAA"
     gene            313..2925
                     /locus_tag="AC16V7_04075"
     CDS             313..2925
                     /locus_tag="AC16V7_04075"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR6256329.1"
                     /translation="MIEWATRPQYSPKAAVYEGRLMEAALLNGLARAGREFGERLFTW
                     HGNLLYLTPVGKDSIPGSWHIAKRRYLERCVDLYGDALATAGTHSNLSLPDPLLAWDF
                     MHLPASERNGYSQAGSLPQHFDEYKSQFYITGTRLTRAFAALFIATSASTPIQSQARD
                     GNPVAVLTEIDSVRNLTFPNPSAIDLPDLYRSYNDYLQLSYDLVRRGVRFGNNNWTPV
                     RARSFAEPVERLIAVTSEQLNDLYARGLYALGENLPADEMARQIEIQNLMARINLPMA
                     RVEVRTDDGGHPLEIEVANLTLKHLLLIRFYADPSFARAFRYDHEDIARARRNEENAA
                     RFGLRSEIENPFTGKPVGMRDFLHWTLLETKPLAEALGLWEDLTPLVEMAAGGPNTAE
                     MLRARLKSELGSEDVVPLELLQQMAEARQSQVQQDVEMIAETFQSLPGDSARLGEFLQ
                     RARDDVHLDMNVPIRFRPKPEALIELTYPDKTSEIIALAEQLIRIPSVTACPEERLDE
                     VRRAATFIYDYARDRGLVVNYFDKDKYPAVFVCFPGQIHAPVMLSGHFDVVPPEPDDS
                     QFEPRIDGNFLLGRGAADMKTVVATYLVWMKDTLRLAPSYPPINLLLIGNEENGEMEP
                     MGTPQALRYLASEAEASSQSYSPQLLIAGERTGERGNELWGEICTQNRGVMRFDVVAR
                     GQRGHSGVAGLSSDLTERLLAARAAITGILSKHLRLSSDDGWHSQARFPFIQVGTPGI
                     YNVTADFGLVGVEVRSIPQDDLNILREELQSYCDSQGLELQIPVMENGVLCDPQNQYL
                     KSLLQAVENLSGEKPRIGKKLPGTSARFAPGGQGVVWGQSGLGPHAANERHFIPSIEP
                     YYRALGEFGRLLKG"
     gene            2922..>3520
                     /locus_tag="AC16V7_04080"
     CDS             2922..>3520
                     /locus_tag="AC16V7_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006622444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HIR6256330.1"
                     /translation="MSIAFLLKGLVIGFSIAAPVGPIGVLCIKRTLTNGRMSGLVSGL
                     GAATADALYGSIAGFGLTLISAFLLDQQTWIRLVGGAFLCYLGLKTFISKPAEQDVRA
                     HGGNLISAYLSTFVLTLTNPLTILSFAAIFAGLGLGATDGDFASAGILVSGVFLGSAA
                     WWLLLSGGVSLLRQRLDQQVMAWVNRASGVIILGFGVAA"
BASE COUNT          818 a          805 c          975 g          922 t
ORIGIN      
        1 taggcgctgc tcaaagatta ttacaaggtc tagaagcaat gtgaagtcca cgtaatctcg
       61 cggggttcaa ccatgtacga gccaaactta tttgcagaga aatatcagct ggcacttgag
      121 acagccctca aagaaaaacc acagggaggt gtgtgtggct ttgagttgga atggaacttg
      181 ctcgatgatc aattccgccc attgctgaca gttggatcag gtccgagtcg tcagtcattt
      241 gtggattatt tgcgggcaga gtgcttgcca tcatggctgg ctgcgtagag ccaattagaa
      301 gtctttcact ggatgatcga gtgggctaca cgcccgcaat actcgccaaa ggcagcagtc
      361 tatgagggcc gcttaatgga agctgccttg ctgaatgggt tagcacgggc tggcagagag
      421 tttggcgagc gcttattcac atggcatggc aatttattgt atttgactcc ggtgggcaaa
      481 gactccatac ctggatcctg gcatattgca aagcgtcgtt atctggaacg atgtgtagat
      541 ctgtatggtg acgcattggc gacagccggc acacatagca acctttcctt gcctgatccc
      601 ttgttggcat gggatttcat gcatctaccg gcaagcgaac gaaacggata tagccaggcg
      661 ggaagcttac cacagcactt tgacgagtat aagagccagt tctacataac cggtacacga
      721 ttaacccgcg cttttgcagc cttatttatc gctaccagcg cgtctacacc aatccaatct
      781 caggcgcgcg atggaaatcc ggttgcagta ttaaccgaga tcgattccgt tcgtaacctc
      841 accttcccca atccaagcgc tattgacctg ccagatttat accgctccta caatgattac
      901 ctccagctat cctatgattt ggtgaggcgg ggtgtccgct ttggcaacaa taactggacg
      961 cctgtgcggg cgcgctcctt tgccgagcca gtagagcggt tgattgctgt aactagcgag
     1021 caattaaatg atctttatgc ccgaggattg tatgcacttg gggagaattt gccagcagat
     1081 gagatggcta ggcagatcga aatccagaat ttgatggcgc gcattaacct tccgatggcg
     1141 cgcgttgaag tccgtacgga tgatggaggg catcccctcg agatcgaagt agcaaatcta
     1201 acgttaaaac atctacttct tatacggttc tatgctgatc catcattcgc ccgggctttt
     1261 cgatatgacc atgaggatat cgctcgcgca cgaaggaatg aagaaaacgc tgctcgattt
     1321 gggctgcgct ccgagatcga aaaccctttt acgggcaaac ctgttggcat gcgtgatttc
     1381 ttgcattgga cattgctaga gacaaaaccg ctggctgaag cccttggtct gtgggaagac
     1441 ttgacgccgc tggtggagat ggctgcaggg gggccaaata ctgccgagat gttgcgcgct
     1501 cgattgaaat cagagctcgg cagtgaggat gttgtgcctc ttgaattatt gcagcagatg
     1561 gcagaagcga ggcaatctca agttcaacaa gacgtagaaa tgatcgcgga aacattccaa
     1621 tcgttgcctg gagattccgc tcgcctgggc gaatttttac agcgcgcccg agatgatgtg
     1681 caccttgata tgaatgtccc catccgtttc cgccctaagc ccgaagcact tattgagctg
     1741 acttatccag ataaaacgag cgaaataata gctctggctg aacagttgat tcgcattccg
     1801 tctgtgactg cttgtccgga agagcgcctc gacgaagttc ggcgagcagc cactttcatt
     1861 tatgattatg cgagagatcg cggcctggtt gttaattatt ttgataagga caagtatccg
     1921 gctgtctttg tatgttttcc gggacagatc catgctccgg tgatgctctc ggggcacttt
     1981 gacgttgttc cccctgaacc cgatgatagt caatttgaac cgcgaattga cggtaatttt
     2041 ttgttgggca ggggggcggc agatatgaaa acagtggtgg caacctacct cgtgtggatg
     2101 aaagatacgc tgaggcttgc tccaagctat ccccctatca atttgttgct cattggcaat
     2161 gaagaaaacg gcgaaatgga gccgatggga acccctcaag cgctgcgcta tttggcaagt
     2221 gaagctgaag cttcatccca atcctactct ccccaattat tgatcgctgg tgagcgcacc
     2281 ggtgagcggg gcaacgaact atggggagaa atttgcacac aaaatcgggg tgttatgcgg
     2341 ttcgatgttg tggcgcgtgg tcagcgtgga cactcggggg tggctggcct tagctccgat
     2401 ttaaccgagc ggttactggc agcccgggct gcaatcacag gaatactttc caaacatctc
     2461 aggctgagta gtgatgatgg atggcattcc caagcgcgtt ttccttttat ccaagtgggg
     2521 acaccaggta tttataatgt caccgccgat tttggcttgg taggcgttga ggtgcgctcg
     2581 atacctcagg atgatctaaa tattttgcgg gaagaactcc agtcatattg tgactcacaa
     2641 ggactggagc ttcagatacc cgttatggaa aatggggttt tgtgcgatcc acagaaccag
     2701 tatttaaaat ctttgctgca agctgtagag aacctatcgg gcgaaaaacc tcgcattggg
     2761 aaaaagctgc ctggcaccag cgcacgattt gcacctgggg gacagggtgt ggtttggggg
     2821 caaagtggtt tgggaccaca tgctgcgaat gaacgtcact ttatccctag catcgagcca
     2881 tattaccggg cgctaggtga gtttggaagg ctcctgaagg gatgagtatt gcctttttac
     2941 tcaaaggctt agtgatcgga ttctcgattg ccgcgccggt tggtccgatt ggggtgttat
     3001 gtattaagcg gacactgacg aatgggcgca tgtctggatt ggtttctggc ttaggagctg
     3061 ccacagccga tgctttgtat ggttccatcg ctggtttcgg gcttacattg atttccgcgt
     3121 ttttgcttga ccaacaaacc tggatacgcc tggttggggg ggcattcctg tgttatctag
     3181 gtttgaaaac tttcatatct aaaccagcag aacaggatgt cagagcgcat ggtggtaacc
     3241 tgatcagcgc ttatctcagt acatttgtgc ttacgctgac caatccattg accatcctgt
     3301 cctttgcagc aatattcgca ggattgggcc tgggcgctac ggatggcgat tttgcatcgg
     3361 caggtatttt agttagtggt gtatttttag gctctgctgc ctggtggcta ttgctgagtg
     3421 gtggcgtcag tttgctgcgg cagaggcttg accagcaggt tatggcttgg gtaaacaggg
     3481 cctcgggtgt gattattttg ggatttggag tggcagcctt
//
LOCUS       DBNHOH010000232         3735 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_462390, whole genome shotgun sequence.
ACCESSION   DBNHOH010000232 DBNHOH010000000
VERSION     DBNHOH010000232.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_462390"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="AC16V7_04085"
     CDS             complement(<1..563)
                     /locus_tag="AC16V7_04085"
                     /inference="COORDINATES: protein motif:HMM:NF013396.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HIR6256331.1"
                     /translation="MGRTVSWNHLVQNLPPELGKAALKAQPLPAIVTGIALDSRQVRP
                     GNVFVALTGGSTDGHGYIPEAVQRGAVVIVGEKPMGGLAVPYLQVAHSREALACLAAA
                     FYDLPARRLTVIGVTGTDGKTTTTNLIYRIMLDAGLRAGMISTVNAVVGDQELDTGFH
                     VTTPEAPDVQNYLAQMVEAGITHVVLEA"
     gene            complement(656..1393)
                     /gene="atpF"
                     /locus_tag="AC16V7_04090"
     CDS             complement(656..1393)
                     /gene="atpF"
                     /locus_tag="AC16V7_04090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR6256332.1"
                     /translation="MVALGLNLGYLLVQVFNFLIIFIVLRAWVVDPIVNILERRRETI
                     AQGLEDARVAAEARANAEVEAREIVAKAQTEANQKVREATERAEAAAREVQARADADA
                     AKAREAALAEAALERDRILADVRGQIAALAMAAAQRLIGDALDESRQRVLIEEFFSGV
                     RTGKVVVLEGEGISGSSAEITSALPLTPAEQEAVKSDVLSKIGSQSTVTFRVDPSILG
                     GLVVRVGDKVLDGSVSGQLETLRQSLR"
     gene            complement(1420..1647)
                     /gene="atpE"
                     /locus_tag="AC16V7_04095"
     CDS             complement(1420..1647)
                     /gene="atpE"
                     /locus_tag="AC16V7_04095"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HIR6256333.1"
                     /translation="MEVAAAKLIGAGLAMIGTIGAGLGIGVLVNGAVQGIARNPDASA
                     NVQTNMILGIAFAEAVAIYCLVVALLILFVG"
     gene            complement(1692..2678)
                     /gene="atpB"
                     /locus_tag="AC16V7_04100"
     CDS             complement(1692..2678)
                     /gene="atpB"
                     /locus_tag="AC16V7_04100"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR6256334.1"
                     /translation="MEQKKNRFFGIPNRYFVLAFIVLGYFAARAFPPILPHIQLPAER
                     VSHDPLFTLPVIGDFYWTNTLWAMLLVDVILIIIALAVRRAASGGSLVPSGFSGAVEA
                     LLEVIYNLTESTSGKWAKSILPFFVTITLMVLVANWIELIPGVDSIGVLEESDHGYAV
                     QEVLPGVATIIKGEEEVEHGGYSLVPYVRVLSTDLNFTVGLALISVIMTQVLGVRSLG
                     GKYFTKFINVGGLFSRPVFGVIDFGVGLLELVSEISKILSFSFRLFGNIFAGSVLLFV
                     IGTLVPVFAQSLFLMLEFFVGLIQAFVFGMLTMVFMAQATAGHGDHGDEQGH"
     gene            complement(2681..2977)
                     /locus_tag="AC16V7_04105"
     CDS             complement(2681..2977)
                     /locus_tag="AC16V7_04105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF021073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HIR6256335.1"
                     /translation="MESTPKQPERSRSRYAINMTLAAVAGQVGCLTLVIVLAALFGGL
                     WLDMRFGTKPMITVVLMVASVPVTLAGMFWIVRMAVSRINSASPANSTPREGEN"
     gene            complement(3096..>3735)
                     /locus_tag="AC16V7_04110"
     CDS             complement(3096..>3735)
                     /locus_tag="AC16V7_04110"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HIR6256336.1"
                     /translation="PLWAAVIAALSTATMTLGNTLALAQRNIKRMLAYSSVAHAGYAL
                     IGLVAVSGLGIASVVFYLVVYVVTNLAAFGVVAAFGKVTGSDEIAAYAGLSRRSPWLA
                     LTFMVALLSLSGMPPFGGFVVKFAVFAAAVQAGQVWLAVVGVLNSIVGLYYYLTVLKV
                     VYLYRSDEEDKPFPISRSYAVALTVLVFGIILLGTVFAPWFSVSNSAAAALF"
BASE COUNT          924 a         1127 c          960 g          724 t
ORIGIN      
        1 gcctccaaga cgacgtgcgt gatacccgcc tccaccattt gggccaggta attttgcaca
       61 tcgggcgctt cgggggtggt gacgtggaat cccgtatcca gctcctgatc tccaacgacg
      121 gcattgaccg tagagatcat ccccgcccgg aggcctgcat ccagcatgat gcggtagatc
      181 aaattcgtcg tggtggtctt gccatccgtg ccggtcacgc cgataaccgt caagcgacgg
      241 gccggcaagt cataaaacgc cgccgccagg caggcaagcg cttcacggct atgcgcaact
      301 tgcaggtagg ggaccgcaag tcctcccatc ggcttctccc ccacgatgac gaccgccccg
      361 cgctgcactg cctccgggat ataaccatga ccgtcggtgc tgccgccggt gagtgcgaca
      421 aagacattac ccggccgcac ctggcgcgaa tccagggcaa tgcccgtcac tatagctggc
      481 aaaggttgcg ctttgagggc cgccttgccc agctcagggg gcagattctg gaccagatga
      541 ttccacgaaa cggtgcgacc catgggttta ttttaactgc caaggcgcaa agcacgccga
      601 ggatggccaa cctccatcgc gtcctttgcg ccttggcggc ttttctaaga ataaactagc
      661 gtaaactctg gcggagggtc tctagctgac cgctcactga gccatccaga accttatcgc
      721 ccacgcgcac caccaggcca cccaaaatgc tcgggtcgac gcggaaggtc accgtggact
      781 gggaaccgat cttgctcaag acatcagatt tcaccgcttc ctgctcggca ggagtgagcg
      841 gcagggcgct ggtaatctcg gcagacgagc cgcttatgcc ctcgccttcc aataccacca
      901 ccttcccggt gcgcactccc gagaagaact cctcgatcag gacgcgctgc cggctctcat
      961 ccaaagcatc gccaatcagc ctctgggcag cagccatcgc cagagcggcg atttgcccgc
     1021 gcacatcggc cagtatgcgg tcgcgttcca atgcggcttc cgccagagcg gcttcacggg
     1081 ctttggctgc gtcggcatcc gcgcgcgcct gcacctcccg tgcggcagct tcagcccgct
     1141 ctgtcgcctc tcgcaccttc tggttggctt cagtctgagc ttttgcaaca atttcgcgcg
     1201 cttcgacctc ggcattggct cgcgcttcgg cagccacgcg ggcatcttcc agaccttgag
     1261 cgattgtctc gcggcggcgc tccagtatgt taacgatcgg gtcgaccacc cacgctcgca
     1321 ggacgataaa aataatcaag aagttaaaaa cctgaactaa caggtatccc aggttaagac
     1381 ctaatgctac caaggcttat ctcctttcac agcttggatt tagccaacaa ataagatcaa
     1441 cagggcgact accaggcagt aaatagctac ggcctcggcg aaggcaatcc ccaagatcat
     1501 gttagtctgg acatttgcag atgcgtccgg gttgcgggcg atgccctgca cggcgccatt
     1561 caccagcacg ccgataccca accctgcacc tattgttcca atcattgcca aacctgctcc
     1621 gatgagtttt gcagctgcaa cttccattgt taatcgatcc tccttgaaac tttagataaa
     1681 gtattgacta tttaatgacc ttgctcatca ccatgatcgc catgcccggc ggtcgcctgg
     1741 gccatgaaca ccatggtgag cataccaaac acaaaggcct gaatcaggcc tacgaaaaac
     1801 tccagcatca aaaagagaga ctgggcgaat acaggcacca gcgtgccgat cacaaacagc
     1861 agcaccgagc cagcgaagat atttccaaaa agacggaagg agaacgaaag gatttttgag
     1921 atttccgaga ccagctccag cagaccgacg ccaaagtcga tcaccccgaa aaccggccgc
     1981 gagaacagcc cgccgacatt aataaatttg gtgaagtact tgccgcctaa agagcgcaca
     2041 cccagcacct gggtcatgat cactgagatc agcgccaggc ctaccgtgaa attcaggtcg
     2101 gtggagagca cgcgcacgta ggggactaat gaataaccgc cgtgctcgac ctcttcttca
     2161 cccttgataa ttgtggcaac cccgggcaag acctcctgca cggcatagcc atgatcggat
     2221 tcctccagga ctccgatgct atccacgcct gggatgagct caatccagtt cgcaaccagc
     2281 accatcaagg tgatggtgac aaagaagggc aggatggact tggcccattt tccggatgta
     2341 gattcggtga ggttataaat cacctcaagc agggcttcca ctgcaccgct gaaaccactc
     2401 ggcaccaggc tgcctccgct ggcagccctg cgcacggcca gcgcgatgat gatcaagatc
     2461 acatccacca agagcatagc ccacagcgta ttcgtccagt aaaagtcgcc aattacgggc
     2521 aatgtaaaga gcggatcatg cgagacgcgc tctgcgggta gttggatgtg tggcaagatg
     2581 ggtgggaatg cccgggctgc gaaataaccc aggacgataa acgccagcac aaaatagcga
     2641 tttggaattc cgaaaaatct gttctttttc tgttccactg tcaattttct ccttcacgcg
     2701 gcgtagagtt tgctggagaa gccgaattga tgcgggagac cgccattcta acgatccaaa
     2761 acattcctgc taaagtgact gggacgctgg cgaccatgag caccacggtg atcatcggtt
     2821 tggtgccaaa acgcatgtcc agccataaac caccaaaaag ggctgctaac acaatcacca
     2881 aggttaggca gcccacttgc ccagccaccg ctgccaacgt catattgatt gcataacggg
     2941 agcggcttct ctcgggctgt ttcggggtcg actccatagt tagcgaattt tatcacatgc
     3001 ggttgcggat cgtcaacaaa tatgataatt ctgatcaatt attcatactg agcggttaat
     3061 ccgcagcatg tgattccaaa atgatcatct atcttttaaa agagcgcagc cgcggccgag
     3121 ttggatacgc taaaccaggg cgcaaaaact gtgccgagca ggatgatccc aaagacaagc
     3181 accgtcaaag ccaccgcata cgaccgcgag attggaaacg gcttatcttc ctcatcggag
     3241 cgatacaggt acaccacttt aagcaccgtc aagtagtagt acaacccgac aatcgagttc
     3301 aatacgccga caaccgccag ccagacttgt cctgcctgca ccgcggccgc gaagacagca
     3361 aacttcacta cgaacccgcc gaatggcggc atgcctgata gcgaaagcaa tgccaccatg
     3421 aatgtcaggg caagccatgg cgatcggcgg ctcaacccgg catacgcagc gatctcatct
     3481 gagccagtca cttttccaaa tgctgccacc acgccgaagg cagccaggtt agtgacgacg
     3541 tacaccacca ggtaaaagac aacgctggca attcccagcc cagagacagc caccagcccg
     3601 atcagggcat aaccggcatg ggctacggat gaataagcca gcatgcgctt aatattgcgc
     3661 tgcgccaggg caagtgtatt cccgagcgtc atggtggcag tggaaagtgc agcgatgacc
     3721 gcagcccaca agggt
//
LOCUS       DBNHOH010000233         3968 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_13471, whole genome shotgun sequence.
ACCESSION   DBNHOH010000233 DBNHOH010000000
VERSION     DBNHOH010000233.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_13471"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="AC16V7_04115"
     CDS             <1..870
                     /locus_tag="AC16V7_04115"
                     /inference="COORDINATES: protein motif:HMM:NF015755.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR6256337.1"
                     /translation="PVDYYAQIDFGAFIRFIDEINGITITIEKRMKVDQIGGKVKLLR
                     PGTYTLNGELALGYARARNTEGGDFDRAFRQQEVIIAIRKRLLESDNLPNLIAKSPVL
                     YQELSSGVHTNLNLDQAIKLAWLSAQIPKENIKRGVIGPPDQVLLVQSPEGDQVLKPI
                     ADQIRLLRDTVFFHNRPESPAAVNLASIDRVTAEAPKISVLNGTTTTGLAASTLEYLQ
                     SQGITAEAGNAEQVYNNTTIFNYTGKIYTVQYLVDLMKIDQNHIFNRYDPTSQVDVVV
                     ILGMDWAANNPAP"
     gene            943..3153
                     /gene="recD2"
                     /locus_tag="AC16V7_04120"
     CDS             943..3153
                     /gene="recD2"
                     /locus_tag="AC16V7_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SF1B family DNA helicase RecD2"
                     /protein_id="HIR6256338.1"
                     /translation="MDTLTGSVERITYYNPENGYSVVRLRPERGKVPGLNRDGLVTVV
                     GNLPELAPGEYLRLSGRWTNHPKHGLQFQSEICEQAQPATLAGIRRYLGSGLIKGIGP
                     KLAERIVDSFGEQTLEVIEEHPERLYEVPDIGPKRSTLIASAWEEQKQVKEIMLLLHS
                     HEVSTNLAVKIYKQYGDQSLEVVQSDPYRLARDIFGVGFKTADRIAQALGLPADHPSR
                     VEAGLIYTLNEMSDEGHVYAPAPELSERAIQLLDVPAELVPPAMERLAKDDRIRQDVL
                     PPPDTTPKGETPALREPAGAYGLPAVYLTPLYHGEVGVAERLRALSQAQPRLNGIRLE
                     PWQNLTSPLSPEQLSAVQAALKHPLSVLTGGPGTGKTTAVQALITALEAGRARLALAS
                     PTGRAAKRLSEATGRPASTIHRLLGYSPGEGFKHNAENPLPVDMLVVDEASMLDLILS
                     NQLLKALQPGTHLLLVGDVDQLPSVGAGDVLRDVIASGVAPVTRLSAIFRQAAGSHII
                     TNAHRINQGQMPLFPSDGDFFLFPGETAEEAGNWAQEVVCERIPKKFGYHPRDQVQVL
                     APLYRGPAGVHALNERLQEALNPPGAMKAEKRLFGQTFRLGDKVMQVQNNYDKDVFNG
                     DIGFVTAFDPIMHSMTVEFEGRKVDYDWSEADQLVLAYAISVHKAQGSEFTAVVLPIV
                     TAHYMMLQRNLLYTAVTRARQLCVLVGSRRAIGMAVKNDKVSRRYTALDWRLRL"
     gene            complement(3260..3553)
                     /locus_tag="AC16V7_04125"
     CDS             complement(3260..3553)
                     /locus_tag="AC16V7_04125"
                     /inference="COORDINATES: protein motif:HMM:NF020317.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HIR6256339.1"
                     /translation="MPIYITMMKLTDQGIKDIKNAPARFAEGVKGLEAVGGKMLGFYL
                     TMGEYDYVAITEGPSDEVALAYLLALGMQGNVRTNTIKAFPQELMAEMVKKLP"
BASE COUNT          872 a         1225 c         1084 g          787 t
ORIGIN      
        1 ccggttgatt attatgccca aatcgacttt ggcgctttca tccgcttcat cgacgagatc
       61 aacggcatca cgatcactat cgaaaagagg atgaaggtgg atcaaatcgg tggaaaggtc
      121 aagttacttc gtccaggcac gtatacgctg aatggcgaat tggccctggg ttatgcccgc
      181 gcccgtaaca ccgaaggtgg cgatttcgac cgcgccttcc gccagcagga agtgatcatt
      241 gcaatcagaa aacggctttt agaatcggac aatttgccta atctgatcgc caagtcgccg
      301 gtgctttacc aggagctctc ctctggcgtg cataccaacc tgaatctgga ccaggctatc
      361 aagctcgcct ggctttcggc gcaaataccc aaagagaaca tcaagcgtgg cgtcattggc
      421 ccacccgacc aggtgctcct ggtgcaatct ccagaaggcg accaggtatt gaagcccatt
      481 gctgaccaaa tccgcctgtt gcgcgatacg gtgttcttcc acaatcgtcc tgagagcccg
      541 gcggcggtca atcttgcatc gattgatcgg gtaactgctg aggcacccaa gatctctgtg
      601 ctcaacggca ccaccaccac tggattggct gcaagcaccc tggaatacct gcaatcacag
      661 ggcatcacag cagaggccgg caacgccgag caggtctaca acaacaccac catcttcaac
      721 tacaccggta aaatctacac cgtccaatac ctggtcgact tgatgaagat cgaccagaac
      781 cacatcttca atcgctatga tccaaccagc caggtggacg tggtggtcat cttggggatg
      841 gattgggctg caaataatcc agcgccataa tcaagcgctg cttgtatcac gtgttccggt
      901 gtcacgtgat aatgaaacac ttggcaccgg agcacgtgac aattggacac attaaccggc
      961 tcagtagagc gcattaccta ctataacccg gagaacggct atagcgtggt gcgcctgcga
     1021 cctgagcggg gcaaggtgcc cggcctgaac cgagatggct tggtgaccgt ggtcggcaac
     1081 ctgcccgagc ttgctccggg ggagtatctg cggctgagtg gtcgctggac caaccatccc
     1141 aagcacggac ttcaatttca aagcgaaatt tgcgaacaag ctcagcccgc cacgctggcg
     1201 ggcatccgcc gttatttggg ctccggtctg atcaagggca ttggcccgaa gctggcagag
     1261 cggatcgtcg acagcttcgg cgagcagacc ctggaggtga tcgaggagca cccagagcgc
     1321 ctgtatgaag tgccagatat cggccctaag cgctcgacct taatcgccag cgcctgggaa
     1381 gagcagaaac aggtcaagga gatcatgctc ctcctgcaca gtcatgaggt gagcaccaac
     1441 ctggcggtga aaatctacaa gcagtacggc gaccagtcgc tggaggtggt gcaaagcgac
     1501 ccctaccggc tggcccgcga tattttcggg gtggggttca aaaccgccga ccgcatcgcc
     1561 caggccctgg gcttgcccgc cgaccaccct tcgcgggtgg aggcaggcct gatctacacc
     1621 ttgaacgaga tgagcgatga agggcatgtc tacgcccccg ccccagagct atccgagcgc
     1681 gccatccagc tgctggatgt gcccgccgag ctagtgccgc ctgccatgga gcgcctagcc
     1741 aaagacgacc gcatccgcca ggatgtattg ccccctcccg atactacacc aaaaggggag
     1801 acccccgcat tgcgcgaacc cgcgggggct tatggcctgc ccgccgttta cctgacaccc
     1861 ttgtatcacg gcgaggttgg cgtcgccgag cggctgcgcg ccctgagcca ggcgcagcct
     1921 cgccttaatg gaatccggct cgagccctgg caaaacctga cctcgccgct ctcgccagag
     1981 cagctttccg ccgtgcaggc tgccttgaaa catcccctca gcgtgctcac cggcggtccg
     2041 ggtacgggca agaccaccgc cgtccaggcg ctcatcactg ccttggaggc cgggcgagcc
     2101 cgcctggccc tagcttcgcc caccgggcgc gccgccaaac gcctctcgga agcaacagga
     2161 cgcccagcca gcaccatcca ccgcctgctg ggctattctc cgggcgaggg cttcaagcac
     2221 aacgccgaga acccgctgcc ggtcgatatg ctggtggtgg atgaggcctc catgctcgac
     2281 ctgatcctct ccaaccagct gctcaaggct cttcagccag gcacgcacct gctgttggtg
     2341 ggtgatgtgg accagttgcc ctccgtgggc gccggcgatg tacttcgcga tgtgatcgcc
     2401 agcggggtcg caccggtcac ccgcctgagc gccatcttcc gccaagctgc cggttcccac
     2461 attatcacca acgcccaccg tattaaccag ggacaaatgc cgctatttcc ctcagatggc
     2521 gattttttcc tcttccctgg tgagacagcc gaggaggctg gcaattgggc gcaggaggtg
     2581 gtctgcgaac gcatccccaa gaaattcggc taccaccccc gcgatcaggt gcaggtgctg
     2641 gcacccttat accgcggccc ggcaggcgtg cacgctttga atgagcgcct gcaggaagcc
     2701 ctaaacccgc ccggtgcaat gaaagccgag aagcgtctct ttgggcagac cttccgcctg
     2761 ggcgataagg tcatgcaggt gcagaacaac tacgataaag atgtgttcaa tggggacatc
     2821 ggctttgtga ccgccttcga ccccatcatg cacagcatga cggtggagtt cgaaggccgc
     2881 aaagtggatt acgactggag cgaagccgac cagctcgtgc tggcgtacgc catctcggtg
     2941 cacaaggcgc aggggagcga gttcaccgcc gtggtgttgc ccatcgtgac ggcgcactac
     3001 atgatgctgc agcgcaacct gctctatacg gctgtcacgc gcgccaggca attgtgcgtg
     3061 ctggtgggca gccggcgcgc catcggcatg gcagtgaaga acgacaaggt ctcacggcgc
     3121 tacaccgccc tcgactggcg gcttagactg taaaactctc ttctactcgg aaatgctctc
     3181 tgtatacctg ggggtggcgc acccggtgta ctgagctacg catccaataa gttacctatt
     3241 aagtccctgc tgcatcagct cacggcagct tctttaccat ctccgccatc aattcctgag
     3301 ggaatgcctt gatggtgttt gtgcgcacat tcccttgcat acccagcgca agcaggtagg
     3361 ctaacgcgac ctcatcgctg gggccttccg taatagcgac atagtcgtac tctcccatgg
     3421 tgaggtagaa gcctaacatc ttcccgccca ccgcttcaag acccttaacc ccttctgcga
     3481 accgtgcggg ggcattcttg atatccttga tgccctggtc tgtcaatttc atcatagtga
     3541 tatagattgg catagatcat tcctcctatc agatcgtaaa ttattaagcc tgcgccaccc
     3601 ccacttcaaa cccgcccagt gcaaggatcg aagccaggga ctcaattgcc atggagtttc
     3661 acactccgtt gagccattga atataaaaat gccactgacc tatcgtcagc ggcacgaagg
     3721 ataaccacgt tgctgcccgc tttcagcgat cactggattg ggcaacgaga agcattatta
     3781 ttatacaaga cggtacatca gagtcaatat agtgaaatat ttcactaaca actcacccct
     3841 taagctcaat tcttcttagg aaaccacggg atgaaggcgc tggtggtgcg gatgtattcc
     3901 gcgtagcccg gcttggcctg ggccaggctt ttctccagca tggctacacc cgagacgcgc
     3961 accagcaa
//
LOCUS       DBNHOH010000234         7520 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_77612, whole genome shotgun sequence.
ACCESSION   DBNHOH010000234 DBNHOH010000000
VERSION     DBNHOH010000234.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7520)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7520)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7520
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_77612"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..591
                     /locus_tag="AC16V7_04130"
     CDS             70..591
                     /locus_tag="AC16V7_04130"
                     /inference="COORDINATES: protein motif:HMM:NF039426.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HIR6256340.1"
                     /translation="MRTTTSTLVDNSFFARIRRNHGLEHATLHILSKRFPRQSMAGYS
                     DWRGFWIIADVPLEEVQIAVETARKRMRGGERELAVHPTCGTNFVVSGILAGIGATMA
                     LFGVGQRKRDWLERMPLVATMATLALIIAQPLGLLIQSRLTTSADLGDMQVVEIFSGR
                     RGPLPAYRVLTRG"
     gene            594..1571
                     /gene="holA"
                     /locus_tag="AC16V7_04135"
     CDS             594..1571
                     /gene="holA"
                     /locus_tag="AC16V7_04135"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR6256341.1"
                     /translation="MADDPPVVYVLHGEDEFGIASFLADLSGKLGDATTAAMNTTRMD
                     GNTMTLEGLREAAMALPFLASRRLVVVTNPRARLNSPEIQEKFFHLLDQVPVSTAVIL
                     VEGRTLKEDDWLLRWAMGAGSRAYVRSFAQQKEGEMVQFILQQARQAGGQFAPAASWR
                     LARMVGSDTRLAFQEVHKLLAYVNYKRPVEPEDVETLSVPVFHDKIFALVDALAVQDG
                     HKASGLLRRFLEEEDPMYVMAMIVRQFRLLLLAREILDQGGMAKDVQSRLKLKRFEAS
                     KITEQARRFSLAALEGVYRSLRDIDEAIKSGRIEDEVALDSMVAGFTTR"
     gene            complement(1558..1824)
                     /locus_tag="AC16V7_04140"
     CDS             complement(1558..1824)
                     /locus_tag="AC16V7_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256342.1"
                     /translation="MNIGMLWFDNDPKTELNAKIERATSYYRNKYGKTPNVCFVHPSM
                     IGASPTSNGNETLKSGNVEVRISPSIRPNHFWIGVNGTLQANES"
     gene            complement(1821..2633)
                     /locus_tag="AC16V7_04145"
     CDS             complement(1821..2633)
                     /locus_tag="AC16V7_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256343.1"
                     /translation="MLSTQERLEIFLEVLERVAPEMGARAMYNLNTPVRDINQSLSGL
                     LERLSPLPPYSALIGMCEDGLPFLLDLSDTSPGSILIIADEQSGQTRLLQTILESACI
                     LNSPAKVVCSVISPNPGQFESLEAMDNCRAILSTYDRAASEMVVELAELADQRIYGRN
                     QGSVELLAIDDLAAFVQNNDHEINCYLKWLVAHGSQGAVWPISSIKMNQLWRIRDGMY
                     EAFGTHFIGRVSTSQLPAGLGAAPPHFEIPGVFSTLMDGEWIHFWVPERNSL"
     gene            2843..3226
                     /locus_tag="AC16V7_04150"
     CDS             2843..3226
                     /locus_tag="AC16V7_04150"
                     /inference="COORDINATES: protein motif:HMM:NF020317.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HIR6256344.1"
                     /translation="MLQLEPNCSVGFVDIRQIEAGANSNGGRKMATYVSLISYTQQGM
                     QKIKESPARLDEAKKVFKSMGAELKAWYLLVGEYDALVISEGPDGETVAKLLLMIGAL
                     GNVRTHTMLAFTENEYRKIVAGLPG"
     gene            complement(3360..4604)
                     /locus_tag="AC16V7_04155"
     CDS             complement(3360..4604)
                     /locus_tag="AC16V7_04155"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HIR6256345.1"
                     /translation="MSSSSILEVELNSLTYGGDALGRLPDGRIVFVPLGLPGEKVAIR
                     LVEDKRRHARGEVVEILRPSPERVEARCRHYGTCGGCHYQHLPYAAQLAAKQAIVAEQ
                     LERIAGISNPPVGQTIASPLAWGYRNFMQFHQTSQGALGLQKPRSNEVFPLQECHLPE
                     EALNTTWPMLEFEPIPGLDRIGLRAGAGDELQLILESKLPETPELSIEDLPLSVVHLS
                     PWGSLLLAGSQFIEIEVLGRPFRVSAGSFFQVNRLVAEAMVEHLLSNLSLETSTTLLE
                     LYCGVGLFSAFLAPRVARIVGVEASPQTCSDFEYNLDEYDNVELYEASAEHVMESLDM
                     HADIVLVDPPRAGLRRDVMDGLLALRPQQIAYISCDPATLARDAQALIDGEYDLVNIT
                     PFDVFPQTYHIESISWWIKSEI"
     gene            complement(4576..5652)
                     /locus_tag="AC16V7_04160"
     CDS             complement(4576..5652)
                     /locus_tag="AC16V7_04160"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HIR6256346.1"
                     /translation="MSRMFVPGPVDVDPQVLYAQTRPMLPHRSKEFEAIFQRASEKAR
                     QLFYTQQRVFLMTSSGSGLQEAAVRNFAKESVLSCVNGAFARRWYETARANGKQADLL
                     EVEWNRPISGDLVASALKSKHYEMLTVVHNETSTGLQNPVQEIAAAARSVSPDTLICV
                     DAVSSLSGAKIEMDAWGIDMLLTSSQKCLALPPGLALGAVSDRALAYAEGVPNRGWYF
                     DLVLMEKHRLKDSTPATPALSLIFALDLQIDRIMAEGLENRFARHSAMAERVQNWAAQ
                     RGLGLHAPEGYRSQTVSAVLNTLEWDIPALNKFLLEREMRIANGYGPLKGKTFRIAHM
                     GETQLDDIQKLLAALDEFIKHPRS"
     gene            5757..7214
                     /locus_tag="AC16V7_04165"
     CDS             5757..7214
                     /locus_tag="AC16V7_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256347.1"
                     /translation="MRRTLIPFVMVLLLACRAASSLPSSLAPATLTPTLPPTVTPAPT
                     YTPSPNLPRATYDVRFHPDGGLYVGDQVSFEVIAPPETNLRGRSAVVEVQGVEDAVFG
                     PVEFERYGIAGRVQSTMTWAWDTSGLLPGDYAVTFSIEPNGPSWTETVALLPEDALPA
                     VEQRARWARAESNCCLLYYITGSEAERDLAELLQMADREAATVEERLDTNLSEVVGLV
                     ILPRILGHGGFANQDLSVSYLDRRYTGGEPATILRHELVHFLDNQLGGELRPTMLVEG
                     LAVYLSGGHYKPEPLLPRAAALLELGWYLPLDELADDFYPSQHEIGYMEAGALVEYMV
                     ERWGWEAFNDFYRDIRSGPDQVEAMDKALVRHFGITFSRLEDDFIGALGEQAVSEAIK
                     DDVRLSVNFYDTARRYQQALDPSAYYLTAWLPNGKEMRERGIVADYLRHPSAPLNVIL
                     ETLLLSADERLRSGDYAEVEMLLQSVNAALDVFAP"
BASE COUNT         1585 a         2019 c         2231 g         1685 t
ORIGIN      
        1 tcactggaat cggcaccctt gaattaaggg ctatgcccat gtttattccc ccagtggtag
       61 aataatcccg tgcgtacaac tacctctacc ctcgtcgata attcattctt tgcccgcatc
      121 cggcgcaacc acgggctgga gcatgccacc ttgcatattc tctccaagcg ctttccccgc
      181 cagagcatgg cagggtattc ggattggcgt ggtttctgga taatagcaga tgtcccgctg
      241 gaggaggtac aaattgccgt tgagaccgcc cggaaacgca tgcggggggg cgagcgcgag
      301 ctggcggtgc acccaacctg cgggactaat tttgttgttt ctggtattct ggcggggatc
      361 ggcgcgacca tggccttgtt cggtgtgggg caacggaaga gagactggct ggagcgcatg
      421 ccgctggtag cgacgatggc cacgctggct ctcatcattg cccagcccct gggtttgcta
      481 atccagtcac gcctgaccac ttccgccgac ctgggggata tgcaggtggt ggagatattc
      541 tctggacggc gcggtccctt gccagcctat cgagtcctga cacgcgggtg agcatggctg
      601 acgacccccc tgtagtctac gtcctgcacg gcgaggacga gttcggcatt gccagcttct
      661 tggccgacct gtctggcaag ctgggggatg ctacgaccgc ggcgatgaac actacccgca
      721 tggatggcaa tacaatgacc ttggaagggt tgcgcgaagc agccatggcc ctgccatttt
      781 tagcctcccg gcggctagtg gtggtgacga acccaagggc gcggcttaat tcccccgaaa
      841 ttcaggagaa attcttccac ctgctggacc aggtgcctgt ctctactgca gtgattttgg
      901 tggagggacg caccttgaag gaggatgatt ggttgttgcg ctgggcgatg ggagcgggtt
      961 ccagagccta cgtgcgctcc ttcgctcaac agaaagaggg ggagatggtt cagtttatac
     1021 tgcagcaagc caggcaagcc ggagggcagt tcgctcctgc ggcgtcctgg aggctggcgc
     1081 gcatggttgg gtccgatacc cgcctggctt tccaagaagt gcacaaactc ctggcttatg
     1141 taaactataa acgcccggta gaacccgaag acgtggagac gctcagcgtc cctgtctttc
     1201 acgataagat ttttgccctg gtggatgccc tggctgtgca ggatggtcat aaagcgagcg
     1261 ggttattgcg ccgttttctc gaagaagaag acccaatgta tgtaatggcg atgatagtgc
     1321 gccagttccg cctgctgctg ctggctcgtg agatactcga tcagggagga atggcaaagg
     1381 acgttcaatc ccggttgaag ttgaaacgct tcgaagccag caagatcaca gagcaggcac
     1441 ggcgcttctc cttagcggcg ctggagggag tttatcgcag cttgcgggat atcgatgagg
     1501 cgatcaagtc tggtcggatt gaggacgaag tagctttgga cagcatggtg gcgggcttca
     1561 cgactcgtta gcctgaagtg tcccattaac gccaatccaa aaatggttgg ggcggataga
     1621 cggggagatg cgcacttcga catttccgct tttcaatgtc tcatttccat tagacgtagg
     1681 tgatgcgccg atcatgctgg gatgcacaaa gcacacgttt ggcgtcttac cgtacttatt
     1741 acggtagtag ctggtagcgc gctcgatctt ggcattcaac tcggttttgg ggtcgttatc
     1801 aaaccagagc ataccgatat tcataggcta ttcctttccg gaacccagaa atggatccat
     1861 tcgccgtcca tcagggttga gaatacccct ggtatttcaa aatgtggggg tgcagccccc
     1921 aatccagcgg gcaattgaga cgtggaaacc cttcctatga aatgggttcc aaatgcttca
     1981 tacatcccat cacgaatccg ccatagttga ttcatcttga tcgagctgat gggccaaacc
     2041 gccccctggg atccgtgtgc gacaagccac ttgagataac aattgatttc gtgatcgttg
     2101 ttctgaacga atgctgccag gtcgtcgata gccaggagct caacactgcc ttgattcctg
     2161 ccatagatgc gctggtcagc cagctcagcc agctcgacaa ccatctcgct ggcagcccgg
     2221 tcgtaggtcg acaggatagc ccggcagtta tccatcgctt ctaaggattc gaactgacct
     2281 ggatttgggc tgatcaccga gcagacaacc tttgctggtg aatttaaaat gcaggcagat
     2341 tctagaatgg tctgcagcag gcgtgtctgt ccgctttgct catcggcgat gatgaggata
     2401 gagcctggtg aggtgtccga taaatcgagc aggaagggta gcccgtcttc gcacatgccg
     2461 atcaaagcag agtagggcgg cagcgggctt agacgttcta gcaagccgct cagggattga
     2521 ttaatatctc tcaccggtgt gttaaggttg tacatggcgc gtgctcccat ttccggggct
     2581 acccgttcga ggacctcaag aaagatttcc agacgctctt gggtggataa catcgcaatt
     2641 gtattataac agaacgtatg ttctatgtca agagatgcgc gcagcaattt cccccctcct
     2701 gtggggggaa gccgctctca ggcgattgcc tgcctgagct ttgacaggca gtatattcgg
     2761 tcttataatt tgagtgagcc acacatctat ttattccaga tcggccacat cactacaatt
     2821 caaaacggat gccactggcg gtatgttaca gcttgaaccc aattgctcag ttgggtttgt
     2881 ggatattagg caaattgagg caggcgcaaa tagcaatgga ggaaggaaaa tggctactta
     2941 tgtgtccttg atcagctata cccaacaagg tatgcaaaaa atcaaagaga gtcctgcgcg
     3001 gctggatgaa gccaaaaagg ttttcaaatc tatgggtgca gagttgaaag cgtggtattt
     3061 attggttggt gagtatgacg cgcttgtcat cagtgaaggt ccggatggtg aaacagtcgc
     3121 taaattgctc ttgatgatcg gcgcgttagg aaatgtgcgc actcatacga tgctggcttt
     3181 cacagaaaac gagtacagga agatcgtcgc tggtttaccg ggataatata aatcgagtaa
     3241 gtcaatcaat ctgagatcag ctgcgctgcc tcatagagca actcctggcg gaaggttatt
     3301 ctgccaggag ttttttgtag gaacagcgag agtatgcagc gagcagcctc accgactatt
     3361 caaatttcag atttgatcca ccagctgatg ctttcgatgt ggtaggtctg ggggaaaacg
     3421 tcgaaagggg tgatgttgac taaatcgtat tccccgtcga tcaacgcctg agcgtcacgc
     3481 gccagggtag ccgggtcgca ggaaatatag gcgatctgct gaggccgcaa ggcgagcaat
     3541 ccatccatca catctcgtct caagccagcg cgtgggggat cgaccagcac gatgtcggca
     3601 tgcatgtcca ggctttccat gacgtgttct gcggaagcct cgtacaactc cacgttgtcg
     3661 tactcgtcca ggttgtattc aaagtcagag caggtctgag gggaggcctc aacccccact
     3721 atgcgcgcca cgcggggcgc tagaaaggcg ctgaacagtc ccacgccgca atacagctcc
     3781 agcagcgttg tgctggtttc cagggataag ttcgacaaaa gatgctcaac catggcctca
     3841 gccaccaagc ggttgacctg gaagaatgac ccggcggaga cgcgaaaagg gcggcccaag
     3901 acttcgatct cgatgaactg gctgcccgct aataaaagcg agccccacgg gctgaggtgc
     3961 acaaccgaga ggggcaagtc ttcaatgctg agctctggag tctctggcag tttgctttcc
     4021 aggatgagct gcagctcgtc cccggcaccc gcccgcagcc cgatgcgatc cagaccggga
     4081 atcggctcga actccagcat cggccaggtg gtgttgagcg cctcttcggg caggtggcac
     4141 tcctggagcg ggaagacctc gttggaacgc ggtttttgga gaccaagtgc gccttgggag
     4201 gtctggtgga actgcataaa gttgcgatag ccccacgcca gaggcgaggc tatggtctgc
     4261 cccacaggcg ggtttgagat gccagcgatg cgctccagct gttcggccac gatggcctgc
     4321 ttggcagcca gctgcgccgc atagggtagg tgctggtaat ggcagccccc gcaggtgcca
     4381 taatgccggc agcgggcttc gacgcgctcc ggcgatggac gcaggatttc caccacctct
     4441 cccctggcat ggcggcgttt gtcttccacc aggcggatag cgactttctc gccgggcagg
     4501 cccaaaggga caaacactat acgtccatcc ggcagcctgc ccagggcatc gccgccgtag
     4561 gtcagggaat tcagctcaac ttcgaggatg cttgatgaac tcatccagcg ccgccagcag
     4621 tttctggata tcgtccagct gggtttcgcc catgtgggca atgcggaagg ttttgccctt
     4681 cagcggcccg tagccattgg cgatgcgcat ctcgcgctcc agcaggaact tgttgagggc
     4741 cgggatatcc cactccaggg tattgagcac tgcggagaca gtctgcgagc ggtaaccctc
     4801 gggggcgtgc agccccagcc cgcgctgcgc agcccagttc tgcacgcgtt cagccatggc
     4861 gctgtgacgg gcaaagcgat tctccaaccc ctcagccatg atgcggtcaa tttgcaggtc
     4921 tagggcgaag atcagggaga gggctggtgt ggcgggggtg gagtctttca ggcggtgctt
     4981 ctccatcaat accaggtcga aataccagcc gcggttcggc acgccctcgg cgtaagccag
     5041 agcccggtca gagactgccc ccaatgccag gccggggggt agcgccaggc acttctgtga
     5101 agaggtgagc agcatatcga tgccccaggc gtccatctcg atctttgccc cgctcagcga
     5161 ggagacggca tccacgcaga tcagcgtgtc tgggctgacg ctgcgggctg ccgcggcgat
     5221 ttcctggacg ggattttgca agccagtgga ggtctcgttg tgcaccacgg tgagcatttc
     5281 gtaatgcttg ctttttagag ctgaagcgac caaatcgccg ctgatggggc ggttccattc
     5341 cacttccagc aggtcagcct gcttgccgtt ggcgcgggct gtttcatacc agcgcctggc
     5401 aaaggcgccg ttgacgcagg agagcacact ctctttggcg aaattgcgca cagcggcttc
     5461 ctgcagcccg ctgccggagc tggtcatgag aaagacccgc tgctgggtgt agaacaactg
     5521 gcgcgccttt tccgaagcac gctggaagat agcctcgaac tccttgctgc ggtggggcag
     5581 catgggtctt gtctgggcat ataaaacctg cgggtcgaca tcgaccgggc cggggacaaa
     5641 catgcgggac ataacgcctc cgagaaacga gcctgtagat tcgagtgggg atatatattc
     5701 agaccccatt gtactattat cttctctcta ttctcgaatc ccttataatt gcccccatgc
     5761 gtcgcaccct gatacccttt gttatggtct tgctgctggc ttgtcgggct gcctcctcct
     5821 tgccatccag cctggctcca gctaccctaa cgcctaccct gccgccaacc gtcacgcctg
     5881 cgcccactta cacgccttcg cccaacttgc caagggcaac ctatgatgtg cgctttcacc
     5941 ccgatggagg cctgtatgtc ggcgaccagg tgagctttga ggtgattgca cccccggaga
     6001 ccaacctgcg gggtcgcagt gctgtggtag aagtgcaggg ggtagaagat gctgtctttg
     6061 gaccggtgga attcgagcgc tacggcattg cggggcgggt gcagtccacc atgacctggg
     6121 cttgggatac gtccggtttg ctgccgggcg attatgctgt gacattctcg atagaaccca
     6181 atgggccaag ctggactgag acggtggcgc tgctccccga agatgccttg cccgcggttg
     6241 aacagcgggc gcgctgggcg cgcgccgaga gcaattgttg cctgctctac tacatcaccg
     6301 gctccgaggc tgagcgcgat ctggctgaat tgctgcagat ggcggatcga gaagctgcta
     6361 cggtggagga gcgcctggat accaatttat ctgaggtggt ggggctggtc atcctgccgc
     6421 gtatcttggg acatgggggc tttgccaacc aggacttatc cgtgtcctat cttgaccggc
     6481 gttacaccgg tggcgaaccg gcaaccatcc tgaggcatga gctggtccat tttttggata
     6541 accagcttgg aggcgagttg cgcccaacga tgctggtaga ggggctggca gtgtacctga
     6601 gcggtgggca ctacaaacca gagccgctcc tgccgcgcgc tgccgccctc ttggaactgg
     6661 gctggtacct ccccctggac gagctggcag atgatttcta tccctcccag cacgagatcg
     6721 gctatatgga ggccggcgcc ctggtggaat atatggtgga gcgttggggg tgggaggctt
     6781 tcaacgattt ttaccgagat atccgctctg gccccgacca ggtggaggca atggacaaag
     6841 cgctggtcag gcattttggg atcaccttct cccggctgga ggacgatttt ataggtgccc
     6901 tgggggagca ggcggtcagc gaggcgatca aagacgatgt gcgcctgagc gtgaacttct
     6961 acgataccgc ccggcgctac cagcaggcgc ttgacccatc ggcctattac ctgaccgcct
     7021 ggctgcccaa tgggaaagag atgcgagagc gggggattgt ggcagactac ctgcggcacc
     7081 catccgcccc gctgaacgtg atcctggaga cactcctgct ctctgcggat gagcgcctgc
     7141 gatcagggga ttatgccgaa gtggagatgc tgctgcagtc ggtgaatgca gcgctggatg
     7201 tctttgctcc ctaaatccca atggatcttc atctgctcca aaatgtccag cgaaaggaaa
     7261 ttaaaaactc tagcaactga ccttcggaca aaagtcttca tgaccgcagt tcatgaagac
     7321 ttttgtccga acctcagtgg tgattgtccc attttcggtt gggttttgag tgatcgccac
     7381 atctggcgat aatgtaatgc aaattgggct aatttacccc catatattgg gtctcgaaag
     7441 tcaccaacca attttgggac agacaccctc agtgaaacct cttgattttt tgtttttttg
     7501 tgctattatg gtatgcgtat
//
LOCUS       DBNHOH010000235         4790 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1359532, whole genome shotgun sequence.
ACCESSION   DBNHOH010000235 DBNHOH010000000
VERSION     DBNHOH010000235.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4790)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1359532"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="AC16V7_04170"
     CDS             <1..501
                     /locus_tag="AC16V7_04170"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HIR6256348.1"
                     /translation="GYSARAELGGGVILTLCHPLDYLRWLLGDVEALWAFAGRLGNLS
                     LDVEDTAEIGLRFADNTLGSVHLDYNQRPSAHTLEIVGSQGTIRWDNADGAVHLHHEK
                     WHVFPVPPSFERNDLFLAEMRHFQAVVRGEEEPLCSLDDGLQALRLALAARQSALECK
                     LIKLDH"
     gene            513..1676
                     /locus_tag="AC16V7_04175"
     CDS             513..1676
                     /locus_tag="AC16V7_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011290458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylyltransferase domain-containing protein"
                     /protein_id="HIR6256349.1"
                     /translation="MDCLAIIPARGGSKGIPRKNILPLAGKPLIAYNIEQAQRSRYIS
                     RLVVSTDDAEIADVARQYGAEVVWRPAKISGDTASSESALLHVLESLRQDEGYLPEVL
                     VFLQCTSPLTLAEDIDGTVQALLDQDADSALAVIPFHYFLWRQAEKDATGINHDKRIR
                     PLRQEREPQYLETGAVYVMRVEGFMRARHRFFGKTAMYIMPAERRLEIDDPVDFQVAE
                     VLLRQEARGAKSARLPEQVAALILDFDGVFTDDKVYVFQDGREAVRCDRFDGMGIGLL
                     KKTGLPIWVLSSESNPVVQARADKLGIPCLHGLQDKGLALGQLLHEQGIDPAQVVYVG
                     NDVNDLPCMAQVGYAVAVADAHPAVLAQANLVLEHSGGNGAIREICELILDSK"
     gene            1686..2558
                     /locus_tag="AC16V7_04180"
     CDS             1686..2558
                     /locus_tag="AC16V7_04180"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0070085 - obsolete glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HIR6256350.1"
                     /translation="MAKAVKIGDRWVGEGHPVFIIAEIGINHNGNLHIAKRLVDVAFM
                     AGCDGVKFQKRTPEKAVPPEFQNVMRETPWGIISYLEYRRMVEFGFKEYDDIDQYCKE
                     KGIIWFASCWDEDSVDFIEQFSPPCYKIASASLTDIPLLEKVKSTGRPVILSTGMSTL
                     EEIAASVELLGTEHLLIAHSTSTYPCPPEELNLRMIETLRERFDCPIGYSGHEVGLQT
                     TYAAVTLGAAFVERHITLDRAMWGSDQAASVEPGGMIRLVRDIRVIERALGDGQKRVY
                     DSELKAREKLRRRN"
     gene            2649..4421
                     /locus_tag="AC16V7_04185"
     CDS             2649..4421
                     /locus_tag="AC16V7_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional sulfate
                     adenylyltransferase/adenylylsulfate kinase"
                     /protein_id="HIR6256351.1"
                     /translation="MSKQSNNLISPYGGKLVNLVVSGEEREELLAKSSRLPSVQISSR
                     SLCDLELLATGAFSPIDRFMRKNDYERVLTEMRLHDGTLFPIPVTLPVDESALPSWGE
                     QITLSDSRNNTLAVMQIEEVYHWDAQREARLVLGTTDPRHPLFSEMVRWGKVYVSGEL
                     KIIDLPKYHDFQELRRTPAQVRAIMGKMGNGNVVAFQTRNPMHRIHEELTKRAAEGVN
                     GSLLIHPVVGMTKPGDVDHYTRVRVYRALAENYYEKNRTLLSLLPLAMRMAGPREALW
                     HAIIRRNYGANYFIVGRDHAGPGNDSHGRPFYGPYEAQMMVSQYANEIGVQPLEFKEL
                     VYLADDERYEEQDKIPEGARIFNISGTQVRDDYLAKGKSLPEWFTRKETAEILQQMYP
                     PRHKQGVCIWFTGLSGSGKSTTAEVLTALLLERGRQVTLLDGDVVRTHLSKGLGFSRD
                     DRDTNILRIGFVAGEISRHGGTVICAAISPYRATRNEARKMVGEEHFIEVFVDTPIDV
                     CEQRDVKGLYARARRGQITGFTGVDDPYEIPVDPEITLYTVEVSPEENAQKIVDYLEE
                     RGYLLPDGSLNAKQEEQQTEPTPA"
     gene            4424..>4790
                     /locus_tag="AC16V7_04190"
     CDS             4424..>4790
                     /locus_tag="AC16V7_04190"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6256352.1"
                     /translation="MRGLPKLFDLTGRVAVITGGAGLLGAEFCLTLAEAGAAVVIADL
                     NEGGALALAESLSQGGKRALAVKTDVTFPDSVQNMVAAALQTFGRLDLLVNSAALDPK
                     FDPQSIKQTGEREIGAKGIE"
BASE COUNT         1052 a         1371 c         1397 g          970 t
ORIGIN      
        1 ggatatagcg ctcgcgccga attaggcggc ggtgtgatcc tgaccctgtg ccaccccctg
       61 gactatctgc gctggctttt gggagacgtc gaggcgctgt gggcttttgc cgggcgcctg
      121 ggcaacctgt cgctagacgt ggaagacact gctgaaattg gcttacgatt tgctgacaat
      181 acgttgggaa gcgtgcattt ggattataat caacgaccgt ctgcccacac gctggagatc
      241 gtgggcagcc agggcacgat ccgctgggac aacgccgacg gagccgtcca cctgcatcac
      301 gagaaatggc atgtcttccc cgtcccgccc agctttgagc gcaacgatct gttcctggca
      361 gagatgcgcc acttccaagc ggtcgtacgg ggtgaagaag agccgctttg cagcctggac
      421 gacggcttac aggctttgcg cctggcattg gctgctcgcc aatctgccct agagtgcaaa
      481 ctgatcaaac tggatcattg accgagtaag agatggactg tctcgctatt atccctgcac
      541 gcggcggctc taagggcatc ccgcgcaaga acatccttcc cctggctggc aaaccgttga
      601 tcgcctacaa cattgaacag gcgcagcgtt ctcgctacat cagccggttg gtggtctcca
      661 ccgacgacgc tgagattgcc gacgtggcgc gccagtatgg ggcggaggtc gtctggcggc
      721 ccgccaagat cagtggcgat accgcctcat cggaatcggc tctgttgcac gttttagaga
      781 gcctgcgcca ggacgaggga tacctaccgg aagtcctggt tttcttacaa tgcacatccc
      841 cccttaccct ggccgaagat atagatggca ccgtgcaagc cttgttggat caagacgcag
      901 acagcgccct ggcggtgatc cccttccatt attttctttg gcggcaggcc gagaaagacg
      961 ctacaggcat caaccacgat aagcgaatcc gtcccctgcg ccaggagcgc gagccacagt
     1021 acctggagac cggggcggtt tacgtgatgc gcgtggaggg tttcatgcgg gcgcgtcacc
     1081 gcttcttcgg caagaccgcc atgtacatca tgcccgccga gcgccggctc gagatcgatg
     1141 acccggtgga ttttcaagtc gccgaggtgc tgctgcgcca ggaagcgcga ggcgccaaaa
     1201 gcgcacgcct gccggagcaa gttgccgccc tgatcctgga tttcgacggc gtattcaccg
     1261 atgacaaggt ttacgttttt caagatgggc gcgaggccgt gcgctgcgac cgtttcgatg
     1321 gtatgggcat tggcttgctc aagaagaccg ggttgcccat ttgggtgctc tccagcgagt
     1381 ctaaccctgt ggtgcaggcc cgtgccgata agctgggtat cccttgcctg catgggctgc
     1441 aggacaaagg tctggccttg ggacaactcc tccatgaaca gggcatcgac cccgcccagg
     1501 tggtttatgt tggcaatgat gtcaacgatc tgccctgcat ggcgcaggtc gggtatgctg
     1561 tggcagtagc cgacgcccat ccggctgtgc tggctcaagc caacctggtc ctggagcaca
     1621 gcggcggcaa tggggcgatc cgagagattt gtgagttaat tctagatagt aagtaaggag
     1681 cctgcatggc aaaagcagta aaaatcggcg accgatgggt tggtgaggga catcccgttt
     1741 tcatcattgc agagattggc atcaatcaca acggcaatct acacattgcc aagcgcctgg
     1801 ttgatgtggc ctttatggct ggctgcgacg gggtgaagtt ccagaagcgc acacctgaga
     1861 aggctgtacc acccgaattc cagaatgtga tgcgcgagac gccctggggt atcatctctt
     1921 acctggaata ccgccgcatg gtcgagttcg gcttcaaaga atatgatgat atcgaccagt
     1981 attgcaaaga gaaaggcatc atctggtttg cctcgtgctg ggacgaggat tcggtggatt
     2041 tcattgaaca attctcacca ccctgttaca aaatcgcttc tgcttcgtta acagatatac
     2101 cactattgga gaaggtgaaa tccaccggac gaccggtcat cctctccacc ggcatgtcca
     2161 ccctggaaga gatcgcggct tctgtagagc tgctgggcac ggagcatctg ctgatcgctc
     2221 acagcaccag tacctaccca tgcccgccag aggagctgaa cctgcgcatg atcgagaccc
     2281 tgagggagcg ttttgattgc ccgataggat actctggtca tgaagtgggt ctgcagacca
     2341 cctacgccgc agtgaccctg ggtgccgcat tcgtggagcg ccatattacc ctggatcgag
     2401 ccatgtgggg cagcgaccag gcagcctctg tagagccagg agggatgatt cgactggtgc
     2461 gcgatattcg tgtcatcgag cgcgccctgg gcgatgggca aaaacgagta tatgacagcg
     2521 agctcaaggc tcgcgagaaa ctgcggagac gaaattgaac caaaatgcaa gaatggcatc
     2581 aagccttatc agttatcttg tgatcatcat gccatagtgc aaaataatta ttcaaggaga
     2641 aacattgtat gtcaaaacaa agtaataacc tcatcagccc ctacggggga aagctggtca
     2701 atttggtggt atctggtgag gagcgcgaag aactgctagc caaatcaagc cgcctgcctt
     2761 ctgtgcaaat ctcatctcgc tcgctttgcg atctggaatt gctggcgaca ggggctttct
     2821 ctccaatcga tcgctttatg cgtaagaatg attacgagcg tgtcctgacc gagatgcgcc
     2881 ttcatgacgg caccctattc cccatcccgg tcacactccc tgtggacgag agcgctctac
     2941 ccagctgggg tgagcagatc acgctgagcg acagccgtaa caatacgctg gccgtgatgc
     3001 aaatcgagga agtgtaccac tgggatgccc agcgggaagc gcgcctggtg ctgggcacca
     3061 ccgacccgcg ccacccgctg ttctcggaga tggtgcgctg gggaaaggtg tatgtctcag
     3121 gcgagctgaa aatcatcgac ctgcccaagt accatgattt ccaggagttg cgccgcacac
     3181 cagcccaggt ccgcgctatc atggggaaga tgggcaatgg caacgtggtt gccttccaaa
     3241 cacgcaaccc catgcaccgc attcacgagg agctgaccaa gcgcgctgcc gaaggtgtga
     3301 acggcagcct gttaatccat cccgtggtgg gcatgaccaa gcctggcgac gtggatcact
     3361 acacccgcgt gcgcgtctac cgcgccctgg cagaaaatta ctacgaaaag aaccgcacgc
     3421 tgctcagcct gctgccgtta gccatgcgca tggctggacc gcgcgaggcg ctttggcatg
     3481 ccattattcg tcgcaactat ggcgctaact atttcatcgt tgggcgcgac cacgctggac
     3541 caggcaatga cagccacgga cgccccttct acgggcccta tgaggcgcag atgatggtaa
     3601 gccaatatgc caacgagatc ggcgttcagc ctctcgaatt caaggagctg gtctatctgg
     3661 cagatgatga gcgctacgaa gagcaggata aaattccgga gggcgcccgc atcttcaaca
     3721 tctctggcac tcaggtgcgc gacgactacc tggcaaaagg caagtcacta cccgagtggt
     3781 tcacccgcaa agagaccgct gaaatcctgc agcagatgta tccaccgcgc cacaagcaag
     3841 gcgtctgcat ctggttcacc ggtctgagtg gctcgggcaa gtccaccacc gcggaggtgc
     3901 tcacagccct gctgctggaa cgcggccgcc aggttactct gctagacggc gatgtagtgc
     3961 gcacccacct ctccaaggga ttgggcttct cgcgtgacga ccgcgacacc aatatcctgc
     4021 gcatcggttt cgtagccggc gagatctccc gccatggcgg caccgtgatc tgcgccgcta
     4081 tcagccccta ccgcgccacg cgcaacgaag cccgcaagat ggttggcgag gagcacttca
     4141 tcgaggtctt cgtagatacc cccatcgatg tctgcgagca gcgtgatgtc aagggcctgt
     4201 atgcacgcgc ccgccgcggc cagatcaccg gcttcaccgg cgtggacgac ccctacgaga
     4261 ttcctgttga tccggagatc accctgtata ccgttgaggt tagccccgag gagaacgccc
     4321 aaaaaattgt cgactacctc gaagagcgtg gctatctgct ccccgatggt tcgttgaatg
     4381 ccaaacagga agagcaacag actgaaccta ccccagcata aacatgcgag gtctaccgaa
     4441 attatttgac ctgacaggcc gtgtcgccgt catcactggc ggcgccggtc tgttgggcgc
     4501 ggaattctgc cttacactgg cagaagccgg ggccgctgtg gtcatcgccg acttgaacga
     4561 gggcggtgcg ctggcgctgg ctgaatctct ctcccaggga ggcaaacgcg ccttggcggt
     4621 gaagaccgac gtgacgttcc ctgattcggt ccaaaacatg gtggcggctg cacttcagac
     4681 ctttgggcgg ttagatctcc tggttaatag cgcagctctc gacccgaagt tcgatccgca
     4741 atcgataaag cagacaggcg aaagggagat aggggcaaag ggaattgaag
//
LOCUS       DBNHOH010000236         2133 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_333535, whole genome shotgun sequence.
ACCESSION   DBNHOH010000236 DBNHOH010000000
VERSION     DBNHOH010000236.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_333535"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1225
                     /locus_tag="AC16V7_04195"
     CDS             173..1225
                     /locus_tag="AC16V7_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256353.1"
                     /translation="MNKIWMLLFGLLLLTMACNLELALPGQPAQPTQVASATSTRRVV
                     ASPSPQAALASPTSAPKPTRTPTLTPVPVPSRTPVTPTEVLPTPPAGRLILIEYFDKY
                     GGDALWMPPDAYIGRHLPRLVIYTDGQLIWRDDAGALWESSMSKDAMCTLVGGMEKFG
                     LFKVEGDGTLEADDPIYEKIPADRLNDTGFVNFLLVVNGNPAKWVIIYRPFVDSLVRP
                     VKSSWELFNSFKPSDIQPYEPELYVMWIEAQQKLARSYGAGADLQPADWPRDLPKLNK
                     LLGDNDAVQIPLDSSEVHSLLKYYDPAPGVKLFKEGRDRYSVILRPLLPHEELNNLSP
                     VPQAAQQFALPFKCPK"
     gene            complement(1298..>2133)
                     /locus_tag="AC16V7_04200"
     CDS             complement(1298..>2133)
                     /locus_tag="AC16V7_04200"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter C-terminal domain-containing
                     protein"
                     /protein_id="HIR6256354.1"
                     /translation="ECAALIGPNGAGKTTFLKTLLGMLAPLAGEVVMGASLNVAYFAQ
                     AHEDLKPERTLVEEIEAVAPGMLLAGIRDFLARFLFSGEEVFKKVSTLSGGERGRLAL
                     AKLALSDANLLLLDEPTNHLDIPSQEILQEVLSEYQGTILLVSHDRYLIDALATQIWE
                     ILPDEASMQIFQGTYSQYRAYLEAEKATAEKAATEVKARHAGLAEKSRPSPEERQRRA
                     KLKELEDRIALLEGHLAALSSQLENPPTDPAKVQRLGGEYVRVQEELEGLMREWEGLH
                     A"
BASE COUNT          444 a          655 c          579 g          455 t
ORIGIN      
        1 aaatagcctt tagcgctcca aatcttagga gaccgttgac tcgagtgcca tgctccccta
       61 taatgggttt gggatcgcgc atacataaag gcacttcctg atccggcggg agcccgggat
      121 ttccctgcac cgccgtatga ctccacaaaa tcaatgcggg aggttggttg ggatgaacaa
      181 aatttggatg ctcctattcg gtctcctgct gcttacgatg gcctgcaacc tggagcttgc
      241 tcttcccggg cagccagcgc agccaacaca ggttgcatcg gcaacctcca cccgccgggt
      301 ggtagccagt ccatcgcccc aagccgcatt agcctcacct acatcggctc caaaacctac
      361 ccgaacccct acattgaccc cggtccctgt gccttccagg acacccgtca cgcctaccga
      421 ggtgctgccc acgcctccag ccggcaggct gatcctgatt gagtatttcg ataagtatgg
      481 cggtgacgcc ctctggatgc ccccagacgc ctacatcggc aggcacttgc ccagattggt
      541 gatctacacc gatggccagc tgatctggcg cgacgacgcc ggtgcgctgt gggaaagcag
      601 catgtccaaa gatgccatgt gcaccctggt ggggggcatg gagaaattcg gcctcttcaa
      661 ggtcgaaggg gacgggacgc tcgaagccga tgacccgatc tacgaaaaaa tcccggcaga
      721 ccggctgaac gatactggtt ttgttaactt cctgctggtt gtcaacggta atcccgccaa
      781 atgggtcatt atttaccggc ctttcgtaga ttctttggtc aggccggtta agtcatcttg
      841 ggagttgttc aacagcttca agccttctga tattcagccc tacgaaccag agctttacgt
      901 catgtggatc gaggcgcagc aaaaactggc ccgcagctat ggtgccggcg cagacctcca
      961 gccagccgat tggcccaggg atttgccaaa attgaacaag ctgctcggcg acaacgacgc
     1021 cgtccagata ccgctggaca gctctgaggt ccactcgctg ctgaaatatt atgacccagc
     1081 cccaggggtc aagttattca aagagggaag ggaccgctac agcgtcattc tgcgcccgtt
     1141 gctgccgcat gaagagttga ataacctttc acccgtgccg caggccgccc aacaatttgc
     1201 cctgcccttc aaatgcccca agtgagggga gcgggctcgc gctaattttg cgaaaatccc
     1261 aggggatgtc gacgcatatt ccagttcgcg tcgagagcta ggcgtgcaga ccctcccatt
     1321 cacgcatcaa accttccaat tcttcctgca ctctcacata ctccccaccc aaccgctgta
     1381 ccttggcagg gtcagtaggc gggttttcta gctggctgga gagcgctgcc agatgacctt
     1441 ccagcaaggc gatgcgatct tcgagttcct tcaacttcgc ccggcgttgt cgctcttcag
     1501 gcgaggggcg tgatttctca gccaatcctg catggcgtgc cttgacctcc gtggccgcct
     1561 tttcagcggt cgccttctcc gcttctaggt aggcgcggta ctggctgtat gtaccctgga
     1621 aaatctgcat ggaagcctca tctggcagga tttcccaaat ctgcgtcgcc agcgcgtcga
     1681 tcaggtagcg atcatgcgag accagcagga tggtgccctg gtactccgag agcacctctt
     1741 gcaaaatctc ctgagacggg atgtccaggt ggttggtggg ctcatccagc aagagcaagt
     1801 tggcgtcact caaagccagt ttggccaggg ccagacggcc gcgctccccc ccagatagcg
     1861 tggagacttt cttgaagacc tcctcgccgc tgaaaaggaa gcgcgccagg aagtcgcgaa
     1921 tacccgccag cagcatgcct ggggcaaccg cctcgatctc ctccaccagg gtgcgctcgg
     1981 gtttcaggtc ctcgtgcgcc tgggcaaagt aagctacatt taagctggcg cccatcacca
     2041 cttcgccagc caggggggcg agcatcccca gtaatgtttt gagaaacgtg gtttttccag
     2101 ctccatttgg accgatcagg gcggcgcact cgc
//
LOCUS       DBNHOH010000237         2285 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_77761, whole genome shotgun sequence.
ACCESSION   DBNHOH010000237 DBNHOH010000000
VERSION     DBNHOH010000237.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_77761"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..1768)
                     /locus_tag="AC16V7_04205"
     CDS             complement(41..1768)
                     /locus_tag="AC16V7_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6256355.1"
                     /translation="MSNNSSQPSWRNILNYGLIGSVITLYLAVIGMVEAFASRYLIGT
                     YISMGQIFFITGAIVSGILAARAFRDKSTLSAVGASLAAGMISSLTSLLLVLFIQVFV
                     IPQAGKDVLFNVRNMFVNFSPGLVEILTFGLGPALGSLALVIISGGLGMIGSVFMLLP
                     SRWRNALISGVAWTLGIGIFSENVAQIVQEIIGRGLVRILFQNKSLNPTAAVVIFVVA
                     FLVGYFRVHRQAGNQWGKLSPAQQKQGRFIGIVLLIAFILALPWMIGLFLSQALFIVG
                     YFIMMGLGLNIVVGYAGLLDLGYVAFFAFGAYTMGVLTSQGPLGVSNANFWVALPISV
                     LVGVIWGLLLGFPVLGMRGDYLAIVTLGFGEIIRILALSDWLAPLQGGAQGVLHIPPP
                     NLFGIQLNTPQSMYYLVVFGSLLAAFVTIRLRESRLGRQWMAMREDEDVAEAMGINLV
                     ETKLLAFAIGAAFSAAAGAIFAARLSNIFPHSFNLIVSINALALIIVGGLGSIPGVIV
                     GGLILVGLPELLREFTEYRLLMYGILLIVMMLVRPEGFLPEATRKRELHAGEENYIEP
                     QAAATEMEG"
     gene            complement(1778..>2285)
                     /locus_tag="AC16V7_04210"
     CDS             complement(1778..>2285)
                     /locus_tag="AC16V7_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6256356.1"
                     /translation="FQVPLVQLVVLFAAILLMIGLYLFVERTKQGKAMRAVSEDKDVA
                     RLMGINIDRVIVTTFAIGGGLAGAAGVFNGLFRPQGIYFFMGFFPGLKAFTAAVLGGI
                     CNVPGAMIGGLLLGVIESIFPNLILEGLGIPAANQLRDAFAFTVLVLILIFRPQGIFG
                     ERLAEKKA"
BASE COUNT          625 a          618 c          602 g          440 t
ORIGIN      
        1 tttatcacct tgcttgtaga taagattgtg cccatatcgc ttaaccctcc atctcagtgg
       61 ctgccgcttg cggctcgata tagttctctt ctcctgcatg caactcgcgc ttgcgggtgg
      121 cttcgggaag gaagccttcg ggtcgcacta acatcatcac gatcagcaag atgccataca
      181 tcaggaggcg atattccgtg aactcacgca ataattcagg cagcccgaca aggatcagcc
      241 cgccaacgat gacgccaggg atgctgccta agccacccac gatgatcaga gccagggcgt
      301 taatggatac gatcagattg aagctgtgag ggaagatatt gctcaggcga gcggcgaaga
      361 tggctccggc tgcggctgaa aatgctgcac cgatggcgaa agccagcagc ttggtttcga
      421 ccaggttgat gcccatggct tcggcaacgt cctcatcctc gcgcatagcc atccactgcc
      481 gtccgaggcg cgattcgcgc agacggatag taacaaacgc cgccaagagc gagccaaaaa
      541 ccaccagata gtacatcgat tgcggggtgt tcagttgtat gccgaaaaga ttcggaggtg
      601 ggatatggag caccccctgg gcgccgcctt gcaggggcgc tagccagtcg gaaagggcca
      661 ggatgcggat aatttcgcca aatcccagcg ttacaatcgc cagatagtcg ccgcgcatac
      721 ccagcacggg aaagccaagc agcaagcccc aaatcactcc caccaatacg ctgatgggca
      781 aggcgaccca aaaattcgcg ttgctgaccc ccagaggacc ttgggaagtg agcacgccca
      841 tcgtgtacgc cccgaaggcg aaaaaggcaa cgtaacccag gtcaagcaga ccagcgtaac
      901 caaccacgat gttcagtccc agacccatca taataaagta acccacgatg aagagagcct
      961 ggcttaaaaa taggccgatc atccagggca gggctaagat aaaagcgatc agcaaaacga
     1021 tgccgatgaa acgcccttgt ttctgttggg ctggtgaaag ttttccccat tggttgcctg
     1081 cttgcctgtg cacccggaag tatccgacca aaaaagcgac tacaaaaatg acaaccgccg
     1141 ccgtagggtt gagagattta ttttggaaca ggatacgcac caaacccctg ccgataattt
     1201 cttgaacgat ctgcgcgacg ttctcggaga agatgccgat tcccagggtc caggcgacac
     1261 cgctgatcaa agcatttctc caccttgaag gcaggagcat gaatactgag ccgatcatac
     1321 ccagcccgcc actaatgatt accagtgcca ggctaccgag agcaggtccg aggccaaatg
     1381 tcaaaatctc gaccagaccc ggcgagaagt tgacaaacat attgcgcaca ttgaagagca
     1441 catccttgcc agcctgaggg atgacgaaaa cctgtataaa gaggactaag agtagcgaag
     1501 taaggctgga gatcattcca gctgccaggc tggcacccac tgcgctcagg gtcgatttat
     1561 ctctgaacgc tctggcggct agtatcccgg aaacaatggc tccggtgatg aagaagattt
     1621 gccccatcga gatataagtt ccaatcaggt agcgtgaagc gaaggcttcc accataccga
     1681 tgacggccag gtagagcgta atcacgctgc cgattaggcc ataatttagg atattccgcc
     1741 agcttggttg tgaactatta ttagacatat tgcaaactca cgctttcttc tcggccaagc
     1801 gttcgccgaa aataccttgt gggcggaaga taaggattag gaccagcacg gtgaatgcga
     1861 acgcgtcccg tagttgattg gctgcaggga tgcccaatcc ctccaagata aggttgggga
     1921 agattgactc gatcacccct aaaagcaagc ccccgatcat ggcgcctggc acgttgcaga
     1981 taccccccag cacggcagct gtgaaggctt ttagaccggg aaagaagccc ataaagaagt
     2041 aaatcccttg ggggcgaaat aagccattga aaacaccggc tgcgccagcc aggccaccac
     2101 cgatggcgaa ggttgtgaca atgacgcgat cgatattaat gcccatcaag cgggcgacat
     2161 ctttatcttc cgaaacagcg cgcatggctt tgccttgttt ggtgcgttcg acgaacaagt
     2221 acaaaccgat catcaagagt atggcagcga ataagaccac aagttgaacg aggggaactt
     2281 gaaac
//
LOCUS       DBNHOH010000238         3906 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1295669, whole genome shotgun sequence.
ACCESSION   DBNHOH010000238 DBNHOH010000000
VERSION     DBNHOH010000238.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1295669"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="AC16V7_04215"
     CDS             <1..192
                     /locus_tag="AC16V7_04215"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015212449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HIR6256357.1"
                     /translation="GPGYYLPKVPYVMYFYKDYGLHGTYWHNNFGRPMSHGCVNLKTK
                     DAGWLYDFASVGTVVNVHQ"
     gene            197..1246
                     /locus_tag="AC16V7_04220"
     CDS             197..1246
                     /locus_tag="AC16V7_04220"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HIR6256358.1"
                     /translation="MNTTRQNQDAKIARSISRREFLKLGGAGILGFLLPGEHADQKTP
                     LEQQGRVLEDGIQLYDIPSFHGRELQKFWKDMVVPISEATIGDSEPAHNRVWYAINGE
                     GFVHSGSIQPVSTELNPELADIPNGGTVAEVTVPYTDAHWRPDKKYTVAYRLYYETTH
                     WVTGLVYDNLGQAWYRIAEDKWKLKYFAPATHLRILPAEELNPISPDLPPFAKRIEVR
                     TQEQVVIAYEWDQPVFMAKTATGAQFSNGNFATPPGRHITAHKRPSRHMAAGNLAYNG
                     YDLPGVPWICYITESGISFHGTYWHNNYGRPRSHGCINLSPKAAKWIYLWTMPMVPPR
                     DQFIRKDFGTIVDVI"
     gene            1247..1831
                     /locus_tag="AC16V7_04225"
     CDS             1247..1831
                     /locus_tag="AC16V7_04225"
                     /inference="COORDINATES: protein motif:HMM:NF016413.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HIR6256359.1"
                     /translation="MMHSAFLYNQYFLKRQVLALTGKIRLYSPDGSLLLYSQQKMFRL
                     REDIRVYNDESRSQELLYIQARNIIDFSAAYDVIDSLSGIKVGVLRRKGLRSLLRDQW
                     EILDASDQPQAILQEDNPTRALLRRLLLGTLLPQNYDVISGETRLADFRQRFNLFRYE
                     LELDFSMDSARRLDRRVGLAAAILLGIIEGRQES"
     gene            1836..2261
                     /locus_tag="AC16V7_04230"
     CDS             1836..2261
                     /locus_tag="AC16V7_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256360.1"
                     /translation="MSRQAIFSGLVIDENDNLVQVRVVGDEPCYVVDDAGFLRHIPSE
                     LVDRQVLEAMGESMQGHEDLISEQAAKMLGQEDIFSIAMIANQIKNIDKQLDSLIETG
                     IPEDVRAYLGMMGFKVRINVHGDVLEVVQPGSAGPPDIE"
     gene            2375..3208
                     /locus_tag="AC16V7_04235"
     CDS             2375..3208
                     /locus_tag="AC16V7_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012314.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6256361.1"
                     /translation="MMASDNIIDVSEADFEYQVLAYSQHIPVVVDFWAEWCVPCRTLG
                     PMLERLTLEAGGAFRLAKVNVDQNQNLSLRYNVRSIPAVKAFRDGKMVSEFVGMQPEP
                     KLKEWFRALAPSSTDLLLEKGLSLISEGEWVSSQEAFQEILKVNPDSAPALFGLAKSI
                     LAQGQVDEAQRILQSLPPSREFARAEALLPLTQALIRQQNGENNHDQPLEAAYQRALR
                     LILRGNIPASLDGLLDILREDKYFRDGEVRQVILGLFELMGDENPVTRQYRQELASVL
                     F"
     gene            complement(3316..>3906)
                     /locus_tag="AC16V7_04240"
     CDS             complement(3316..>3906)
                     /locus_tag="AC16V7_04240"
                     /inference="COORDINATES: protein motif:HMM:NF018190.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HIR6256362.1"
                     /translation="FDEWDFDRYSVSRVVYGDNRWNGNDDLSATLMIGWDDYYLYLAA
                     RVKDDIYVQKASGKNLFKGDSIEILLDANVSRDYNDNDLSNDDYQIGISPGQSTPSDD
                     IETYLWYPSSKAGEYTSIKAAALPTDDGYRIEAKIPWEIFGITPDIGKHFGFAFSVSD
                     NDKSSENVQQSMVSNVSTRHLTDPTTWGDLTLEGRR"
BASE COUNT         1037 a          924 c          951 g          994 t
ORIGIN      
        1 ggaccagggt attatctccc caaagtgccg tatgtcatgt atttctacaa ggactacggg
       61 ctgcacggta cttactggca taataatttt ggaagaccaa tgagtcacgg ctgtgtcaac
      121 ctgaaaacca aggacgccgg ttggctatat gactttgcct ccgtcggcac ggtggtcaac
      181 gtccaccagt aaactcatga ataccacgcg gcagaatcag gacgcgaaga tagcccgatc
      241 tatctcccgc cgtgaattct taaaacttgg gggggcggga atattgggtt ttctcttgcc
      301 gggagagcat gccgaccaaa aaacacctct ggagcagcag ggtcgagtcc tggaagatgg
      361 catccagcta tatgatattc cttcttttca cggaagagag cttcaaaaat tctggaaaga
      421 catggtcgtc ccgatctccg aagcaaccat tggagatagc gaacctgccc acaaccgcgt
      481 gtggtacgca attaatggcg aaggcttcgt ccactctgga agcatccagc cagtatcgac
      541 tgagctcaac cctgagcttg cagatatacc aaacggcgga acggtggctg aggtaacggt
      601 cccgtataca gatgcccact ggcgtccgga taaaaaatat acggtagctt atcgactata
      661 ttacgaaacc acccattggg taacaggtct ggtttacgac aatttgggtc aagcgtggta
      721 tcgaattgcc gaagataagt ggaaattaaa gtattttgcg cctgccaccc atttgcgcat
      781 actacctgct gaggagctta acccaatatc gcctgactta ccaccatttg ccaagcgcat
      841 cgaagtgcgg acgcaagaac aggtggtcat cgcttacgaa tgggaccagc ctgttttcat
      901 ggccaagact gccacgggag cacaatttag caacggcaat tttgccactc ctccggggcg
      961 ccacatcacc gctcataaac gcccatctcg gcacatggca gctgggaatc tggcgtataa
     1021 tggttatgac ctacctggag tgccgtggat ttgctacatc acagagagtg gaatctcgtt
     1081 ccacggtaca tattggcaca acaattacgg tcgaccccgc agtcatggct gcatcaatct
     1141 atctccaaag gctgccaagt ggatttacct ctggacgatg cctatggtgc cacctaggga
     1201 tcagtttatc cgcaaagatt ttgggacaat cgtggatgtg atttaaatga tgcattcagc
     1261 atttctctac aaccagtact tcctcaagcg acaggtgttg gcactaaccg ggaaaattcg
     1321 cttgtactct ccagatggtt ccctgttgct ctattcccaa caaaagatgt ttcgcctcag
     1381 ggaggatatt agagtttaca acgatgagag ccgcagccaa gagctactct acatccaagc
     1441 gcgcaatatt attgattttt cagcagccta tgatgtgatt gatagtctat ccggcatcaa
     1501 agtaggcgtt ctcagacgta aaggtttgcg ctccttgttg cgtgaccagt gggagatatt
     1561 ggacgcgagc gatcagcctc aggccatttt gcaagaggac aatcccacgc gcgccctatt
     1621 gcgccgatta ttgctgggta ctctattacc ccaaaactac gatgttattt ctggtgaaac
     1681 cagattagca gattttcgcc agcgcttcaa ccttttccgt tacgaattgg agctggactt
     1741 tagtatggat tcagctcgca ggttggacag gcgggtgggg cttgccgctg ctatcctgct
     1801 gggaatcatt gaagggcgcc aggaatcatg acctaatgtc ccgtcaggca attttttccg
     1861 gtttagtcat tgacgagaat gacaacctcg tacaggtgag agttgtgggg gatgagccct
     1921 gttatgtggt ggatgacgct ggttttttac gacacatccc ttcagaacta gtggatcgcc
     1981 aggtgctcga agcgatgggc gagagtatgc aagggcatga agatttgatc agtgagcagg
     2041 cagcaaaaat gcttggtcag gaagatatct tttccatcgc catgatcgcc aaccagatta
     2101 agaacataga taaacagctg gactccttga ttgagactgg tatccctgaa gatgttcgcg
     2161 cctatctcgg catgatggga ttcaaagtac gaattaacgt ccacggtgat gtccttgagg
     2221 tcgtacaacc agggtcagca gggcctcctg acattgagta agcaccctga taaattcgca
     2281 aagtccccta ccaaattatg tagatttcat ataattctct tacgaaggga tgttaaaatg
     2341 caattgttgg tgaattaatg atggagaaca taaaatgatg gcctcagata atatcattga
     2401 tgtatcagaa gcagattttg aatatcaggt actcgcatat tctcagcaca tacccgtagt
     2461 agtggatttt tgggctgagt ggtgtgtccc ctgtcgcacg ctggggccta tgctggagcg
     2521 tttaaccctg gaagccggag gcgctttccg tttagccaaa gtaaatgtgg atcaaaatca
     2581 aaacctatct ttgcggtata acgtacgcag cataccagca gtgaaagcat tccgagatgg
     2641 caaaatggta tctgaatttg tcgggatgca acctgaacca aagctgaagg aatggtttcg
     2701 ggctcttgcc cctagctcca cagatttgtt gctagaaaaa ggtttaagct tgatatctga
     2761 gggcgagtgg gtatcttctc aggaagcatt ccaggaaata ctcaaggtca accctgattc
     2821 cgcgcccgcc ctgttcggtc ttgcgaagag catattagcg caagggcagg ttgatgaagc
     2881 gcaacgcatc ctgcaatcct tgccccctag ccgcgaattt gccagggcag aagccttgct
     2941 accccttacc caggcgctca tacgccagca aaatggcgaa aacaatcatg atcaaccgct
     3001 tgaggcagct tatcagcgcg cccttcgtct tatcttgcgc ggtaatatcc ccgcaagcct
     3061 agatggactt ctggatatcc tcagggagga caagtatttt cgtgatggcg aggttcgaca
     3121 agtaatctta ggcttatttg agttgatggg agacgagaac ccagttacac gccaatacag
     3181 gcaagagctg gcttccgtgc ttttttagct cacagtaatt catttccaaa ataaaaaaat
     3241 acgcataagc aaaggactag atgggtgttg aacatccacc tagtcctttg ttatgaatgc
     3301 atcatatata aaatgttatc tgcgtccctc caaggttagg tcaccccagg tcgttgggtc
     3361 agtcaagtgc cttgtagaga cgttagacac catggattgc tgcacatttt cactgctttt
     3421 gtcattatcc gagacagaaa aggcgaatcc gaaatgcttg ccaatatctg gcgtaatacc
     3481 gaaaatttcc caaggaattt ttgcctcaat gcggtatcca tcgtctgttg gcaatgcggc
     3541 agcctttatg gaagtatatt cacctgcctt agaggacggg taccataaat aggtttcgat
     3601 gtcatcgcta ggcgtggatt gccccggaga tattccaatc tggtaatcat cgttactaag
     3661 atcattatcg ttataatctc tggaaacatt agcgtccagt aaaatctcaa tgctatcacc
     3721 tttaaaaagg ttcttgccac tggctttttg gacgtaaata tcgtctttga cccgggcagc
     3781 aaggtaaagg taataatcat cccagccaat cattagggtt gcggataggt catcattccc
     3841 attccagcga ttgtcgccat aaactacgcg agaaacggaa tagcggtcaa aatcccattc
     3901 atcaaa
//
LOCUS       DBNHOH010000239         2171 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_77949, whole genome shotgun sequence.
ACCESSION   DBNHOH010000239 DBNHOH010000000
VERSION     DBNHOH010000239.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_77949"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /locus_tag="AC16V7_04245"
     CDS             complement(<1..1227)
                     /locus_tag="AC16V7_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR6256363.1"
                     /translation="MNPEVRVDPVRLEVFKHLYASIAEEMGVVLRKASYSPNIKERRD
                     FSCAIFDERGTMISQAAHIPVHLGSMPLSVRSAISAFTSASHPEGFRPGDMVILNDPY
                     RGGTHLPDITLIAPVFLQSTGGDENLMGYVASRAHHADVGGIAPGSMSIAREIFQEGI
                     VIPPIKLFDGWQINQSVLDLILSNVRTPQERLGDLWAQVAANQRGVTRFLEIINRYGV
                     HEARLYMQLLLAYSERMTRRLLEAIPDGEYLFEDYLDDDGINLEPVPIRVKITVSGDE
                     AIFDFSGSGKQVQGSLNAVYAITLSAVNYVVRCLVGLDVPNNGGCLAPVKLIAPRGTV
                     VNAEHPAPVAGGNVETSQRIVDVLLGALALACPGRVPAASQGTMNNITIGGWDPDQNN
                     LFTYYETVGGGMGARPT"
     gene            complement(1224..>2171)
                     /locus_tag="AC16V7_04250"
     CDS             complement(1224..>2171)
                     /locus_tag="AC16V7_04250"
                     /inference="COORDINATES: protein motif:HMM:NF039581.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR6256364.1"
                     /translation="ELPTVTDANLVLGRLDPDYFLGGKMPLEAERSRQALHKLGLSLG
                     LDSVNAALGVIEVANAHMERALRLISVERGYDPAEFTLLSFGGAGGLHACMLAKKVGM
                     HRIIIPPYASVLSALGMLAANVIKDYSLTVMLPGDTQIDTIESAYAPMVERAMLELQK
                     EGFSEDLIHIERNLDMRYKGQSYELIVPFSDRCLEEFQALHHKEYGYFRAEAPIEIVN
                     LRVRSIGKVVPPSLRLNKDAGLDPARAYIGSRPVIFHEGSLPTPFYRGEELLPGNQIA
                     GPAVIVRDDTTILLLDDVSGSVDTFGNLWLQIHSEEYGQ"
BASE COUNT          576 a          560 c          510 g          525 t
ORIGIN      
        1 ggtcgggcgc gcccccatgc ctcctccaac cgtctcgtaa taagtaaata gattattctg
       61 gtctggatcc caccccccaa tggtgatgtt attcattgtg ccctggctgg cagctggaac
      121 acgtccaggg caagccaatg ccagcgcccc cagaagtaca tctacgatgc gctgagaggt
      181 ttctacgttt cctcctgcca ctggagcagg atgttctgca ttcaccactg tccctctagg
      241 ggcaatcaac tttacagggg caaggcaacc accgttattg ggcacatcta aaccaaccaa
      301 acagcgcact acataattga cagccgataa agtgattgca tagactgcgt tgaggctgcc
      361 ctgaacctgt tttccagacc ctgaaaaatc aaagatcgct tcgtcgccag agacggtgat
      421 tttcacacga attggcactg gctccaaatt tatcccatcg tcgtccaaat aatcttcgaa
      481 tagatattca ccatctggga tagcctccaa tagacgacgg gtcatcctct cggagtatgc
      541 cagtaataat tgcatataca agcgagcttc atgaacaccg tagcgattga ttatctctaa
      601 gaaacgggta actccccttt ggttagcggc aacctgcgcc cataaatcgc ccagacgctc
      661 ttgaggcgtg cgcacattcg aaaggatcaa gtcgagtaca gattggttaa tttgccaacc
      721 atcaaacagc tttataggcg ggataacaat cccttcttgg aatatctccc gtgcaatgga
      781 cattgagcct ggcgctatgc cgcctacatc agcatggtgg gcacggctgg cgacatatcc
      841 catgagattt tcatctcctc cagttgactg cagaaacact ggtgcgatta gggtgatgtc
      901 cggcaagtgt gtcccgcctc gatagggatc gttgagaatg accatatcgc cagggcggaa
      961 tccttcaggg tgggaagcag atgtaaaagc gcttatcgct gatctgaccg agagaggcat
     1021 agaacccaaa tggacaggta tgtgagccgc ttgcgagatc atggtccctc tttcgtcgaa
     1081 tatcgcgcag gagaaatcgc ggcgttcttt gatattgggg gagtagctag ctttacgcag
     1141 gacgaccccc atctcctctg caattgaagc gtagaggtgt ttaaagacct ctaagcgtac
     1201 ggggtctacc ctcacctctg ggttcattgt ccgtattcct cgctgtgaat ttgcagccaa
     1261 aggttaccaa atgtgtccac tgatccgcta acatcgtcca aaagcaaaat ggtcgtatca
     1321 tccctgacaa taacagccgg ccctgcaatt tggtttccag gcaataattc ctctccacga
     1381 taaaaggggg ttggaagtga tccttcgtgg aatataacag gtcgagaccc aatataggca
     1441 cgtgccgggt ccaggcctgc gtctttattt aaccgcagtg acggtggtac taccttgcca
     1501 atagagcgta cccgcaaatt gacaatctca attggcgctt ctgcacgaaa atacccatat
     1561 tccttgtgat gtaaagcttg aaattcttca agacagcgat ctgaaaacgg aacaattaac
     1621 tcataagatt gccccttata gcgcatatcg agattgcgtt cgatatgaat tagatcttct
     1681 gaaaaacctt ccttttgcaa ttcaagcata gcgcgttcta ccataggagc ataggcactc
     1741 tcaattgtgt ctatttgagt atcgccgggc aacatcacag tcaatgagta gtctttgatt
     1801 acattggcag ccagcatgcc cagggcagag agtacagatg cgtagggagg gatgattatc
     1861 ctgtgcatcc cgactttttt agctaacata caggcatgaa ggccgccagc accaccaaag
     1921 gaaagcaggg taaattcggc gggatcgtaa ccacgctcaa cagagataag cctcagagcg
     1981 cgttccatat gcgcattggc aacttcaatc actccaaggg cagcattcac gctgtccaat
     2041 ccgagggata agccaagctt atgaagcgct tgtcttgatc gctcggcctc taatggcatt
     2101 ttaccgccca agaaataatc tgggtccaaa cgaccaagaa ctaagttagc atcagtcaca
     2161 gttggtaact c
//
LOCUS       DBNHOH010000240         2240 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1167605, whole genome shotgun sequence.
ACCESSION   DBNHOH010000240 DBNHOH010000000
VERSION     DBNHOH010000240.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1167605"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="AC16V7_04255"
     CDS             complement(<1..751)
                     /locus_tag="AC16V7_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR6256365.1"
                     /translation="MKRSAWRFLWYAKDIRRKLLVTVGLLAIFRLAAHVPVPGANREA
                     IASIIAGGGAGGTLFSLLNMLSGGTVTNFSVLAMGVYPYITAQIILQLLVPIIPALQR
                     RMEEDPREGQKWMEKWTYMLAVPMAALQAIGQINLFNAFTGGVPIISDFGFSGANLLP
                     SITIILSMTAGTMFAIWLGELISEYGIRNQGLSLIIFAGIVSDVPTNLLRLLQDERTR
                     VFVPIFIAIMMALIIFAIVYVQQGRRNVPVMY"
     gene            complement(797..1252)
                     /gene="rplO"
                     /locus_tag="AC16V7_04260"
     CDS             complement(797..1252)
                     /gene="rplO"
                     /locus_tag="AC16V7_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIR6256366.1"
                     /translation="MKLHDLQPNPGSKKSRKRVGRGTAAGQGKNAGRGVKGQGARAGG
                     GVRLYHQGGNLPFYRRLPFVRGQGFTPLFRQEYNEVNLDQLADFPADSEVTPETLETA
                     RLLHEPENPVVILGRGEVSVALKVSAHRVSKGAQAKIEAAGGSVEILEL"
     gene            complement(1257..1463)
                     /gene="rpmD"
                     /locus_tag="AC16V7_04265"
     CDS             complement(1257..1463)
                     /gene="rpmD"
                     /locus_tag="AC16V7_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HIR6256367.1"
                     /translation="MARKAKVVEKRLRVTLVKSPIGYTQRQKDTVRALGLHRMHATVE
                     HIDSASVRGMIAKVSHLVDVEEVE"
     gene            complement(1456..1983)
                     /gene="rpsE"
                     /locus_tag="AC16V7_04270"
     CDS             complement(1456..1983)
                     /gene="rpsE"
                     /locus_tag="AC16V7_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIR6256368.1"
                     /translation="MTDYNEFDQLELDERVVEIARVAKVVKGGRRFSFRVTVVVGDNR
                     GSIGVGIGKANAVPDAMRKATDKARRNMQKVNMFGTTIPHEVMGIMAGSKVLLKPASP
                     GTGVIAGGGVRAVLEAVGVSDILTKSLGSSNVLNVVYATINALEQLKSPQEVAAFRGK
                     AVKDVTPFWERRKNG"
     gene            complement(2017..>2240)
                     /locus_tag="AC16V7_04275"
     CDS             complement(2017..>2240)
                     /locus_tag="AC16V7_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004101467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIR6256369.1"
                     /translation="GHTLVSASSVDRHLRDTLKGKKKSEQAHLVGKALAERAKEKGIS
                     IVVFDRGGYRYIGRVKALADGAREGGMEF"
BASE COUNT          505 a          660 c          551 g          524 t
ORIGIN      
        1 gatacatcac tggaacgttt cgccgcccct gttgtacata gacgatggcg aatatgatca
       61 aagccatcat gatggcgatg aagatgggca cgaagacccg ggtgcgctca tcctgcaaca
      121 agcgtagcag gttggtgggc acatccgaca caatacctgc aaagatgatt agggaaagcc
      181 cctggttgcg aataccgtac tctgagatga gctcacccaa ccagatggcg aacatcgtgc
      241 cagcggtcat gctgaggata atcgtgatcg acggcaagag gtttgcgccg ctgaagccaa
      301 agtcgctaat gatcggcaca ccacctgtga aggcattgaa caaattaatc tgtccaatcg
      361 cctgtagagc tgccatggga actgccagca tatacgtcca cttctccatc cacttctgcc
      421 cctcgcgtgg atcttcttcc atccgcctct gcagagcagg gataatggga accagaagct
      481 gcaggataat ctgtgctgtg atgtagggat agacccccat cgccaagacg gagaaattgg
      541 tcaccgttcc accggacaac atattgagca ggctgaataa ggttccaccc gcaccgccac
      601 cggcgataat actggcaatc gcctcccggt tcgctcctgg tacaggcaca tgcgctgcca
      661 agcggaatat ggccaacaaa ccgactgtta ccagcagctt gcggcggata tctttggcat
      721 accagagaaa gcgccaagca gaacgcttca tttttggctc ctatgggtaa ataccaaggt
      781 gttaattacc ttcatttcac agctcaagaa tttcaacact accaccggcc gcttcgatct
      841 tggcttgggc acccttgctg actcggtgag cgctcacctt gagggcaaca ctcacctcac
      901 cacgtccgag gatgactacc gggttttcgg gttcatgcag caaacgagct gtctccaacg
      961 tttccggggt cacttcgcta tctgcgggaa aatctgcgag ctgatccaaa ttaacttcgt
     1021 tgtactcctg gcggaacagg ggagtgaaac cctgaccacg gacaaatggc agacggcggt
     1081 agaagggtag attaccaccc tggtggtaaa ggcgtacacc gcctccggcg cgcgcacctt
     1141 gacccttgac accccgtccg gcattttttc cctggccggc ggcggtgccg cggccgacgc
     1201 gcttacgcga tttttttgat cctgggttcg gctgtaaatc gtgcagtttc attttattat
     1261 tccacttctt ccacatctac taggtggctc acttttgcga tcatgccacg caccgaggcc
     1321 gaatcgatat gttccacagt cgcgtgcatg cggtgaagtc ccagtgcgcg cacggtatct
     1381 ttctggcgct gagtgtagcc gatgggactt ttgaccagcg tgacacgcag tctcttttca
     1441 acaacctttg ctttcctagc cattcttcct ccgctcccag aacggggtga cgtccttcac
     1501 cgcctttcca cggaaggcag ccacttcttg aggcgatttg agctgttcca atgcgtttat
     1561 ggtagcgtag accacgttca gcacattgct gctgcccaaa gatttcgtca ggatatccga
     1621 tacgccaact gcctcgagca ctgcgcgtac gccgccaccc gcgatgacgc ctgtacctgg
     1681 ggaggctggt tttagcaaga ctttcgaacc agccataatc cccatcactt catgggggat
     1741 ggtcgtgcca aacatgttca ccttttgcat attgcggcgc gccttatcgg tagctttgcg
     1801 catggcatct ggcaccgcat tggccttacc aataccgaca ccaatgctcc cgcgattgtc
     1861 acccacaacc accgttacgc gaaatgaaaa gcgcctacca cccttaacca ctttggcaac
     1921 ccgggcgatt tcaacgacac gctcgtcgag ttctaattga tcaaattcgt tatagtcggt
     1981 catatatttc tccgtgcagc tggctttaag cccctgctaa aattccattc cgccttcgcg
     2041 agcgccatcc gcaagcgctt taacccggcc aatgtagcga taaccgcccc ggtcgaagac
     2101 aactattgag atgccttttt ccttggcgcg ttctgccaat gcctttccga ccaggtgagc
     2161 ctgctcgctc tttttctttc ccttcagtgt atcgcgcaag tggcgatcaa cactggaagc
     2221 agagacaagc gtatgaccag
//
LOCUS       DBNHOH010000241         2149 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1424350, whole genome shotgun sequence.
ACCESSION   DBNHOH010000241 DBNHOH010000000
VERSION     DBNHOH010000241.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1424350"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            250..774
                     /gene="lspA"
                     /locus_tag="AC16V7_04280"
     CDS             250..774
                     /gene="lspA"
                     /locus_tag="AC16V7_04280"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR6256370.1"
                     /translation="MRKYLRDYGYLLSLAALIITLDQWTKALVRSNLDFEEIWSPWHW
                     LLPYARIVHWKNTGAAFGMFQGFGGVFTVLAVVVALAILYYFPQVPRQDWTLRLAMGM
                     QLSGALGNLIDRLTQGYVTDFISIGTFPVFNVADSSISIGTAILILGMWYRERHTMPV
                     EPASPISPEEIQGE"
     gene            767..1699
                     /locus_tag="AC16V7_04285"
     CDS             767..1699
                     /locus_tag="AC16V7_04285"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR6256371.1"
                     /translation="MSDRLINLIYQEKEAQRLDKFLVSRLPDLSRARIQGLIKDGFVY
                     VDGLEAHKSGQIVDRGSVVRVSIPPPEPTTLEPEAIPLAIVFENQDLIVVNKPAGMVV
                     HPGAGHTRGTLVHAALAHAPDMAGIGGEGRPGVVHRLDKDTSGLILLAKNDAAHRWLQ
                     EQFRQRQVVKGYMALVDGHPPTPSGRIEAPIGRDPAQRRQMAVVAAHKGRAAVSEYHT
                     LESFPKHALLEVHPITGRTHQIRLHLSFLGCPVVGDSIYGRRKPSLPISRHFLHAARL
                     TIRLPKENAPRTFEAQLPTELVQILDDLRSSSEL"
     gene            1696..>2149
                     /locus_tag="AC16V7_04290"
     CDS             1696..>2149
                     /locus_tag="AC16V7_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HIR6256372.1"
                     /translation="MNYIDLRSDTVTKPTPAMWEAMARADVGDDVYGEDPTINRLQEM
                     AAERVGKEAALFLPSGTMGNLSAILAHCGRGDEVILGDKAHSFLLEAGGISALGGIHS
                     RQLPNQPDGTLALDDIRLAIRSDDAHFPISRLVTLENSHNRCGGAALSV"
BASE COUNT          466 a          623 c          587 g          473 t
ORIGIN      
        1 ttcggttgaa aatgtgctat ccttcatagg ggatgctcct ttcggtaagc gcggttgtga
       61 gaacctactt taccgaatta gcatcctttt ttcaatacct ttctccaaaa ggccaaactc
      121 gagcccaaac actctttttg cgggattgcg catgcccttc cgcgcatatg caattgccct
      181 gggcattgaa tcgcgttaac tcatctgagg taaaatgagg gggacaattt gaattcttgg
      241 gagcctgttt tgagaaaata tttgagagat tatggttact tactgagcct ggccgcgcta
      301 atcattacat tagaccaatg gacaaaggcg ttggtgcgtt ccaacctgga cttcgaagag
      361 atttggtcgc cgtggcactg gctgctgccc tatgcccgaa ttgtgcattg gaagaatacc
      421 ggggcagctt ttggcatgtt tcagggcttc ggcggtgtgt ttactgtttt ggctgtcgtc
      481 gtcgccctgg ccatcctgta ctatttcccc caggttcccc gccaagactg gacgctgcgt
      541 ctagcgatgg gcatgcaact tagcggtgcc cttggcaatc tgattgacag gctgacacaa
      601 ggatacgtga ctgatttcat ctccattggc actttcccgg ttttcaatgt ggccgattcc
      661 agcatttcga tcgggacagc gatcttaatc ctggggatgt ggtatagaga acggcacaca
      721 atgcctgtcg aaccagcctc acccatatct cccgaggaaa tccagggtga gtgatcgtct
      781 tatcaacctc atatatcagg aaaaagaagc ccagcgcctg gataaatttc tggtgagccg
      841 cttgccggac ttgtcccgcg cccgcatcca gggattaatc aaagatggct ttgtttatgt
      901 agatgggctg gaggcacaca agagcgggca gattgtggat cgaggtagcg tagttcgggt
      961 gagcatccca cccccggaac ccaccaccct ggagccggag gctatcccgc tagcgatcgt
     1021 tttcgaaaac caagacctga tcgtagtgaa taagcctgct ggcatggtgg tgcaccctgg
     1081 ggcgggtcat acccgtggca ccctggtaca cgccgccctg gcacacgccc ctgacatggc
     1141 gggcattggt ggcgaggggc gtccgggcgt ggtccatcgc ctggataaag acacttctgg
     1201 cctgattttg ctcgctaaga acgacgccgc ccaccgctgg ctacaagagc aattccgcca
     1261 gcgccaggtg gtcaagggct atatggcgct ggttgacggt cacccaccca cacccagcgg
     1321 caggatcgag gcgcctatcg ggcgagaccc agcccagcgt aggcagatgg cagttgtcgc
     1381 tgcgcataag ggacgagccg ccgtcagcga ataccacacg ctggaaagct ttcctaaaca
     1441 cgccctactg gaggtgcacc cgatcaccgg gcgcacacac cagatccgcc tgcatttgag
     1501 cttcctgggc tgcccggtgg tgggtgactc gatctacggg cggcgcaaac ccagcctgcc
     1561 tatctcgcgc cactttctgc acgcagcccg cttgaccatc cgcctgccca aagaaaacgc
     1621 cccgcgcaca tttgaagccc aactgccaac agagctcgtg cagatattag acgatttaag
     1681 atcatcgagt gaactatgaa ctatatcgac ctgcgctcag ataccgttac aaaaccaacc
     1741 ccagccatgt gggaagccat ggcgcgcgcc gacgtaggcg acgatgtgta cggtgaagac
     1801 cctaccatca accgcctgca agaaatggca gccgaacgag tgggtaaaga agcagccctt
     1861 ttcttgccct ccggcacgat gggtaacctg tctgctatcc tggctcactg cggacggggg
     1921 gatgaggtca tcctgggtga caaagcccac agtttcctgc ttgaggccgg gggcatctct
     1981 gccctgggag gcatccattc tcgccagctt ccaaaccagc ccgatggcac cctagcactg
     2041 gacgacatcc gtctcgccat ccgctccgac gatgctcact tccctatatc gcgcctggta
     2101 acgctggaga actcccacaa tcgttgtggt ggcgcagcgc tcagtgtcg
//
LOCUS       DBNHOH010000242         2659 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_269779, whole genome shotgun sequence.
ACCESSION   DBNHOH010000242 DBNHOH010000000
VERSION     DBNHOH010000242.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_269779"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            317..874
                     /gene="efp"
                     /locus_tag="AC16V7_04295"
     CDS             317..874
                     /gene="efp"
                     /locus_tag="AC16V7_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR6256373.1"
                     /translation="MIDVNALRKGVTFELDDALYKVLDFNHHKPGRGNATIRIKAINL
                     RTGATIDKTFQSGERVQDVRLDYHNVQYLYSDGALYHFMDLETYEQPVISGDVLGDSA
                     EYLKEGMEVKLTFYQGEPLDIELPTSVDLLVTQADIAVRGDTATGVTKKVTTETGLQV
                     SVPSFVEEGNIIRVDTRTGAYVTRV"
     gene            1227..2450
                     /locus_tag="AC16V7_04300"
     CDS             1227..2450
                     /locus_tag="AC16V7_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR6256374.1"
                     /translation="MSLTLLLDLDDTLLVNPLKQFLPAYLSKLSTHLAPYAEPSLMVQ
                     ALLAGTRQMVENQQPDCNLRDVFGSVFYPSLKVLPQEIQPAIDQFYTDVFPSLKQLTQ
                     IKPEAVELVDKAFERGYRVAISTNPLFPQTAIVQRLEWAGLSVQKYPFVLISSFESFH
                     FSKPNPAFFAEMMARMGWPEDPVLVVGNDLENDIRSATQLGLPTYFVLQPGTPLPVGE
                     GSAPQNTRLRPTASGDLAGLLSWLDETPFETLQPRYDEPSAILAVLRSTPAALNTMCD
                     TLAPAILARRPKPAEWSPSEILCHLRDVDNEVNLPRLKKVLQEDNPFIPGADADRWAE
                     ERAYHQQNGLAALHRFSTSRQELIHLMEKMSPDDWGRPARHAIFGPTNIKELVTINAS
                     HDQIHIRQMVEALRN"
BASE COUNT          682 a          699 c          629 g          649 t
ORIGIN      
        1 acagacgcat gtcgagccgc ctcaattgat gcgattatcc gaacaattga acatgcaaga
       61 acgtccgctc aacgtgcagg aatttattga attttggtgc gcttaacgct cagtatcgac
      121 ccctcaattc ctcaaccctt gccacaccta tttgactaag ctataatctc cgcctatctg
      181 gtaaaatcca gatgacgatt gtcattaata acgcagttgg aaaagtgatg ggggcttgtg
      241 ctggctctgc ccaaccaatc tctccatcag tcccaaccaa ctgcgtaagt ctgataaatt
      301 atgtgaggct ggttttatga ttgacgtgaa cgcattgcgt aaaggagtta ccttcgaact
      361 cgacgatgca ctctacaagg tgctggattt taatcaccac aaacctggtc gagggaacgc
      421 cacaattcgc attaaagcca ttaaccttcg tactggagcc accatcgata aaaccttcca
      481 gtctggtgaa cgcgtacagg acgtgcgcct agattaccac aacgtacaat acttgtatag
      541 cgacggtgcg ctttaccact tcatggatct ggaaacctat gagcaaccgg ttatttctgg
      601 agacgtgctg ggagacagcg ctgaatacct gaaagaaggt atggaagtca agcttacttt
      661 ttaccagggt gagccccttg acattgaatt acctacctcg gtagacctgc tggtcaccca
      721 ggcagatatc gccgtgcgcg gcgacactgc cacaggtgtc accaaaaagg tgaccaccga
      781 aaccggtttg caagtgagcg tgcccagctt tgtcgaagaa ggaaatatta tccgcgtcga
      841 cacacgtacg ggcgcatatg tcactcgcgt ttgagcatgc gcacacctgc tggatcagag
      901 tgccgctact tctatgggga ttactaccgc ggcagaaaac acgaagaatg ccgcctattg
      961 aatgattcat ctcctcctct gccatggaag ccagcatatt gttccacttg cccggtacca
     1021 gggattcttc gagcaaacgc gtgcaacgat ttatatttaa caccccgcct ggggcgccca
     1081 tttccattcc tgaaacagca ggtcgaggtg gatacctatt gcgagaaaac ccagcgagca
     1141 ggttttgatc ctcacatcgg ctgtggtgaa tgccatccta ttccctcaat atttactgag
     1201 ggagatttac ttagtggaga cgagaagtga gcctcacctt gctactggat ctggatgaca
     1261 ctctgcttgt caatccgtta aaacagttcc taccagcata tctgagcaag ttatccaccc
     1321 acctggcgcc atacgccgag ccatccttaa tggtgcaggc gttactggct ggtactcgcc
     1381 agatggtgga gaatcagcaa ccagattgta acctgaggga tgtatttggt tcggtattct
     1441 acccatctct taaagtacta ccccaagaga tccaaccagc cattgaccag ttttacacgg
     1501 acgtttttcc atccctaaag cagctcaccc agattaagcc agaagccgtc gaactcgtag
     1561 ataaggcctt tgagcgagga tatcgcgttg ctatatccac caaccctctc tttccccaaa
     1621 cagcgattgt ccaacgcttg gagtgggctg gtttgtccgt gcagaaatat ccattcgtcc
     1681 tgatcagttc ttttgaaagc tttcacttct ccaagccaaa tccggcattt tttgcagaga
     1741 tgatggctcg catgggatgg ccagaagatc ccgtattggt tgtgggcaat gacctggaaa
     1801 atgacatacg aagcgcaacg cagcttggat tgccaactta ctttgtgttg caacctggca
     1861 ccccgctccc tgttggagaa ggcagtgcac cgcaaaatac tcgccttcgt ccaacggctt
     1921 ccggggattt agccggactg ctttcctggt tagatgaaac tccgtttgaa actctccaac
     1981 caaggtacga tgaaccatca gccatcctgg ctgttctgcg atctactcct gcggcattaa
     2041 atactatgtg tgatactctt gcccccgcaa tcttggcaag acgtcctaaa ccggcagagt
     2101 ggagtcccag cgagatactt tgccacctgc gtgatgtaga taatgaggta aacctgccgc
     2161 gcctgaaaaa agttctccag gaagacaatc ccttcatccc tggggcggat gcagaccgat
     2221 gggcagaaga gcgtgcttat catcaacaga atgggctggc tgcattacac cgtttctcta
     2281 cctcacgcca agagctcatc cacctgatgg agaaaatgtc cccagatgat tggggtcgcc
     2341 cagctcgtca cgctattttt ggtccgacaa atatcaaaga actggtcacg ataaacgcaa
     2401 gccacgacca gatccatatc cgccaaatgg tagaagcgtt acgcaattga tcgaagttct
     2461 gtggaactcg aaactgcgcc tcgattctct cccattggtg ttttaacaaa aagtcggcgg
     2521 aagtgctaag aaattttcca cagcggtcag cgcagcgccg tgtttcgttg ctgaccgcta
     2581 tatgttgatt gatataatta ggacttacgc agttgaagag gaaaggaggg gggtttgggc
     2641 gggcgatgcc cgcccaaac
//
LOCUS       DBNHOH010000243         1795 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_333801, whole genome shotgun sequence.
ACCESSION   DBNHOH010000243 DBNHOH010000000
VERSION     DBNHOH010000243.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_333801"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="AC16V7_04305"
     CDS             complement(<1..230)
                     /locus_tag="AC16V7_04305"
                     /inference="COORDINATES: protein motif:HMM:NF014679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR6256375.1"
                     /translation="MQPLAIVTDSTSDIPDELIAQHHIQVVPNYVVIDGESAEDGKGM
                     TREAFYTKLPTMISFPTTATASSGTYQQLYNRL"
     gene            complement(232..>1795)
                     /locus_tag="AC16V7_04310"
     CDS             complement(232..>1795)
                     /locus_tag="AC16V7_04310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HIR6256376.1"
                     /translation="QIVNSLNVFPVPDGDTGTNMVLTLQSAMNEISESSESNFGRMAH
                     SVAHGALMGARGNSGVILSQLWRGFARAVDHQEILDATAMAHAFAISRDTAYKGVVRP
                     VEGTILTVSKDIATAAEAALETTNDPFEILEKVVQEASLSVERTPDLLPVLKEAGVVD
                     AGGKGLFFILEGMLRYANGQPLDSALTTVQPLAAMNLENAIESIEPGQDYEVVVDFRP
                     ATALDLESFYSHLEELGTSIQVGEGDGIYRMHIHVPTENLYEPIDYTRSLGVITKVSI
                     ENLIDQMQTIEAIRGGSRLTLAPVEPGQIAVVAVSPGPGISRVFASLGAAAIVDGGQT
                     MNPSTQEIINAFENLPTDQVIILPNNKNIILAAKSASELTVKKVAVVPSCTIPQGLAA
                     MLRLAPDGNFDKVAQEMIEALSDVESGEITTATRSVEIDGVAVREGQIIVLHNGKLVM
                     AADDLEEACLGLLKIAHADHFELITIFYGADMSKNEVNHIVDAIRRDYPGQEIEVQDG
                     GQPHYQFIIAIE"
BASE COUNT          407 a          453 c          464 g          471 t
ORIGIN      
        1 aggcgattgt agagttgttg atatgttcct gaagaagccg tggcagtggt agggaaacta
       61 atcattgttg gaagtttcgt gtaaaaggcc tcccgtgtca tgcctttgcc gtcttctgca
      121 ctctctccat caattacaac atagttggga acaacctgaa tgtgatgttg agcaattaac
      181 tcatcgggta tatcggaggt gctgtctgtt acgatggcga gaggttgcat gctattcaat
      241 ggcgatgatg aactgataat gtggctgtcc gccgtcttgg acttctatct cttgcccagg
      301 ataatcacgg cggatggcat cgacgatatg attgacctca ttcttggaca tgtcggcgcc
      361 atagaatatg gtgatcagct caaagtgatc agcatgcgca attttcaata atcccaggca
      421 agcctcttcc aggtcgtctg cagccatgac cagcttgcca ttgtgcagca cgatgatttg
      481 cccctctcgt actgcgacgc catcgatttc taccgaacga gtggctgtgg taatctcgcc
      541 actctctaca tcacttaaag cttctatcat ctcctgtgct actttatcga aatttccatc
      601 aggagcgaga cgcaacatgg cggctagccc ttgagggatg gtgcagctgg gtacaaccgc
      661 caccttcttc accgtgagtt cagaagcgga tttggctgcc aggatgatat ttttattatt
      721 gggcagtatg ataacctggt cggttgggag attctcaaat gcattgatga tctcctgtgt
      781 gctggggttc attgtctgac ccccgtccac aattgctgct gctcccaggc tggcaaagac
      841 acggctaatg ccgggaccgg gtgagacagc aactacggcg atctgcccag gctcgaccgg
      901 ggctagtgta agccggctac caccgcgaat ggcttcaatg gtttgcatct ggtcaatgag
      961 gttttcaatg gaaactttgg ttatgacgcc caacgagcga gtgtagtcaa tcggctcgta
     1021 gagattctct gtaggtacat gaatgtgcat gcggtaaatg ccatccccct cgcccacctg
     1081 gatcgaggtg cctagttcct ctaggtggct gtagaagctt tccagatcca aggcggtcgc
     1141 tggacgaaag tccaccacga cctcgtaatc ttgaccgggc tcaatagact ctattgcgtt
     1201 ctcaaggttc atcgctgcta aaggttggac agttgtcagg gcgctgtcca aaggctgccc
     1261 attggcgtaa cggagcatgc cttctagaat gaagaacaag cctttccctc cagcatcgac
     1321 tacaccagct tctttaagta ctgggagcag gtcaggggta cgctccacag acaaactggc
     1381 ttcctgaacc actttttcta ggatttcaaa tggatcgttg gtggtctcaa gggctgcttc
     1441 tgcggctgtt gcgatatctt tggatactgt cagtatggtg ccttctaccg ggcgcacgac
     1501 acctttatag gccgtatcgc gagaaatggc aaaagcatgc gccatggcgg ttgcgtccag
     1561 gatctcctga tgatctacag cccgggcaaa gcctctccag agctgtgaca gaatcacccc
     1621 tgaattgccg cgcgccccca tcagtgcccc atgagcaact gagtgtgcca tccttccaaa
     1681 gttgctctcg gaagattctg aaatttcatt catggcagat tgcagagtta agaccatatt
     1741 ggttccggtg tctccatcgg gtaccggaaa tacgttgagt gaattaacaa tctgc
//
LOCUS       DBNHOH010000244         6949 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_78047, whole genome shotgun sequence.
ACCESSION   DBNHOH010000244 DBNHOH010000000
VERSION     DBNHOH010000244.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6949)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6949)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6949
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_78047"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1475
                     /locus_tag="AC16V7_04315"
     CDS             117..1475
                     /locus_tag="AC16V7_04315"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR6256377.1"
                     /translation="MKTIKFVLINPTSPLWRVRGGERPRGSRYFRFSMLPSLYVAASM
                     PANVETQIIDEDVESIDFDVDADLIGVSFMTYNAPRAYEIADIFRTQKGKPVIFGGYH
                     PTFMPEEAIQHADAVCIGEAESNVPRMIDDFLAGDLKPFYHSEPVRLEALPKLNRNLI
                     RKKDYAPVDVLQATRGCFYRCSFCSVAAFHQYCFRTRPVADVIAELKTLGPHVLFMDD
                     NLIGDREYARELFSAMIPLGKRWFSQCGVGIASDDELLCLAARSGCGGLFIGFESLSQ
                     KGLRSWKKRTNLGKDYMEVVQRLHQAGMAVFAGFVFGGDEDTPDVFEQTLEFLLEANI
                     ETLQATRLTPFPGTTLFEELDGQGRIFDKDWSHYDFNHVVFEPRQMDRETLDRGVAWV
                     LREFHTRSRVARRLWKSLGYLHPQVVLRGVLPLNVGFRYKLSMDGNFQRGVDFAPPSM
                     MP"
     gene            1543..2334
                     /locus_tag="AC16V7_04320"
     CDS             1543..2334
                     /locus_tag="AC16V7_04320"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6256378.1"
                     /translation="MKHVVITGSTRGLGFGLADAFLELGCSVTVSGRMQSSVAAAVGK
                     LNEKHDSSRISGCACDVRHLEQIRALWDASIERFKQVDVWINNAGFSGPQLSAWKIPP
                     DQAQDIIQTNLLGVIYGSMVSIQGMLGQGYGSLYNMEGMGSDGRKHNGLALYGTTKYG
                     VHYFTECLVNETRGTPLVVGSIRPGMLITDMILKQYEDGSPDWERAKRVFNLLADRVE
                     TVAPWLAEQILANQRSGACIRWLTKGKLMRRMLFSPFQKRDLFEV"
     gene            2336..2455
                     /locus_tag="AC16V7_04325"
                     /pseudo
     CDS             2336..2455
                     /locus_tag="AC16V7_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015166760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
     gene            2485..2916
                     /locus_tag="AC16V7_04330"
     CDS             2485..2916
                     /locus_tag="AC16V7_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR6256379.1"
                     /translation="MFLYYFSGGLLVFSTVLYHVTQKMTPQDANPALALTVTYMSSLI
                     LCILLFFLFPVSSNLRTAFSQLNWASFGLAFALLGLEVGFLLAYRAGWNISIAAIFVN
                     VAGILLLVPIGLFFFKERLSLINLLGILVCIAGLIMVNWKK"
     gene            3030..3392
                     /locus_tag="AC16V7_04335"
     CDS             3030..3392
                     /locus_tag="AC16V7_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012694571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HIR6256380.1"
                     /translation="MAKILVHVTYGPEHPTRAALAFLVAKSAIDEGHTVTLFLAGDAI
                     QLLRDAVLDTLAGLGTGKLRDSYDAIVAGGGRFYLSGMSSKARGLNEADLAGKPAEFA
                     MPNVLVRLSLENDRMFTY"
     gene            3401..4309
                     /locus_tag="AC16V7_04340"
     CDS             3401..4309
                     /locus_tag="AC16V7_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006675886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6256381.1"
                     /translation="MKPLTNKVAVVAGATRGAGRGIACMLGEAGATVYCTGRSTRARL
                     KSRRSGNMAPFALEGRPETIEETAELVSRYGGVGIPVQVDHTDEEQVEHLFVQVQGEQ
                     GKLDILVNDIWGGDEVSEWGQPFWELSIQKGLLMQQRGVHTHIITSRYAAPLMVARRR
                     GLIIEITDGDSAAYRGNLFYDLAKSSVIRLAYAMAEELRPHNITALALTPGFLRSEAM
                     LEHFGVTEANWQEGATKDPHFIASETPYYIGRAVAALAADPNVFQMSGKALSSWNLAL
                     EYGFKDIDGRQPNWGKYFEEHIAGKM"
     gene            complement(4447..4842)
                     /locus_tag="AC16V7_04345"
     CDS             complement(4447..4842)
                     /locus_tag="AC16V7_04345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR6256382.1"
                     /translation="MPFYQYHCLNCRRRFDTFMSYSEYGTKSVHCPNCNSDQVRRRIG
                     RVRVARSEESRMEDLADPGNLAGLEDDPKALGRMMRKMSREMGEDMGPEFDDVIDRLE
                     SGQSPEEIEKAIPDLGGEGGGAGGLDEDF"
     gene            5030..5653
                     /locus_tag="AC16V7_04350"
     CDS             5030..5653
                     /locus_tag="AC16V7_04350"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR6256383.1"
                     /translation="MTERNIFNSHQDQPLLIVISGPSGAGKDTVIEHMKQRELPFYFV
                     VTATTRPPRPNEVHGVDYFFFSKDEFAGMIERGELMEYALVYKDYKGVPKEQVRRALA
                     SNQDVVMRLDVQGAATVRKLAPEAVLIFLTTSSEEELITRLEARMTESPEGLKLRIAT
                     ARSEMGRLAEFDYVVVNAQNQLDETVDTILAIIKAEHHRVLPRMVNL"
     gene            5650..6462
                     /locus_tag="AC16V7_04355"
     CDS             5650..6462
                     /locus_tag="AC16V7_04355"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR6256384.1"
                     /translation="MNLQDANTPAPGPDNQPGQETAPATAGGQRYVVVKMPPTKPYVT
                     YAFMGFTILVYLLQLGSKFFLGGLDYPAALGLKANDAILQGELWRLFTPMFLHSTSSL
                     LHIGFNMYALYALGPELERHFGHLRYLALYLLSGFAGNVASFLLSPEYSLGASTAVFG
                     LIGAQGAFLYHNRSLFGRIAQRALVNILVVAGINLFIGLSPGIDNWGHVGGLVGGTLF
                     ALFGGPLLQVEAEMGALKLSDRREPGAWVAAGLGVGLLFAALAAVKFFLSGG"
     gene            6486..>6949
                     /locus_tag="AC16V7_04360"
     CDS             6486..>6949
                     /locus_tag="AC16V7_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003326317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6256385.1"
                     /translation="MRVLVTGGAGYIGSHTCVELLQAGYEAVVVDNLSNSKEEALRRV
                     EEITGKRLEFHQVDLLDEAALDAVFRQNPVEAVLHFAGFKAVGESVAKPWSYYHNNVT
                     GTLVLLKVMEQHAVKDIVFSSSCTVYGDPHKVPITEDFPLSATNPYGRTKLI"
BASE COUNT         1480 a         1779 c         2001 g         1689 t
ORIGIN      
        1 acccttacga agcaggctct tgatgcggtg tggctttcaa tcgcctcaac cagacaactt
       61 ttcgcaaaat tgatgccatg ggaggtaatt gggtgggaag aagtaggcta ccaaatttga
      121 agaccattaa gtttgtgctc atcaatccaa cctccccatt atggcgcgtc cgcggtggag
      181 agcgcccacg cggctctcgc tatttccgtt tttccatgct cccaagcctc tacgtggcag
      241 cctcgatgcc agccaatgtt gagacacaaa ttatcgatga agatgtggaa tccattgact
      301 ttgatgtgga tgcggacctc atcggggtgt ccttcatgac ctataatgcg ccgcgagcct
      361 atgagatcgc ggatatattc cgtacccaga aaggcaagcc cgtgatattt ggaggctatc
      421 acccgacctt tatgcccgag gaggctatcc agcatgctga cgcggtttgc attggagagg
      481 cggagagcaa cgtgccgcgc atgatagacg actttttagc tggagatctc aagcccttct
      541 accacagcga gccagtcagg cttgaggcgc tgccaaaact aaatcgcaat ctcatccgca
      601 agaaggacta tgccccggtc gatgtgctcc aggccacgcg cggctgcttc taccgctgca
      661 gtttctgctc cgtggcagcc ttccaccagt attgtttccg cacccgcccg gtggctgacg
      721 tcattgctga gctcaagacg ctcgggcctc atgtgctctt catggatgat aacctgatcg
      781 gtgatcgaga atacgcccga gagttattct ctgccatgat cccacttggc aaacgatggt
      841 tcagccaatg cggggtgggt attgcctccg acgatgagct gctgtgcctg gcagcccgca
      901 gcggatgtgg cgggctgttt atcggctttg agtccctgtc gcagaagggt ttgcgttcct
      961 ggaagaagcg cactaatctg gggaaagact acatggaagt cgtgcagaga ttacaccagg
     1021 cggggatggc ggtgttcgct ggctttgtct ttggtgggga tgaggatact ccggacgtgt
     1081 ttgagcaaac cctggagttt ttactggagg ccaatatcga gacgttgcaa gccaccaggc
     1141 tgacgccatt cccaggtaca acactcttcg aggagctaga tgggcaggga cggatcttcg
     1201 ataaggattg gagtcattac gatttcaatc atgtcgtatt tgagccgcgg cagatggatc
     1261 gtgaaacgct tgatcgtggg gttgcatggg ttctgcgcga gtttcacacg cgtagccggg
     1321 ttgcccgcag gctctggaag agcctgggct acctgcatcc ccaggtggtt ttgcgcggag
     1381 ttttgcccct gaatgtcgga tttcggtaca aactttccat ggatgggaac tttcagcgcg
     1441 gggtggactt cgccccgcct tctatgatgc catgataaac gcaggaagtt atctggcggt
     1501 tgacaggcga taattcttag gggagaatag cagctataaa ctatgaagca tgtcgtgatc
     1561 acaggcagca cacgtggcct aggttttggg ctggcggacg ctttcttaga gcttggatgc
     1621 tcggttactg ttagcggtcg gatgcagtcg agcgtcgctg cagctgttgg caagctaaat
     1681 gagaagcatg atagcagccg catctctggc tgtgcttgtg atgttcggca tctcgagcaa
     1741 atcagggcgc tgtgggatgc gtctatcgag cgcttcaagc aagtggacgt atggataaac
     1801 aacgctggct tcagcggacc ccagctgtca gcctggaaga tcccacccga tcaagcccag
     1861 gatatcatcc agaccaatct attgggcgtc atatatggct cgatggtctc catccagggt
     1921 atgctggggc aaggctatgg cagcctctac aatatggaag gcatgggcag tgatggccgc
     1981 aagcataatg gcctggcctt gtatggaacg accaagtatg gtgtgcacta tttcaccgag
     2041 tgcttagtaa atgagacccg gggcactccg ctggttgtgg gttcaatccg tcccggcatg
     2101 ctcatcaccg atatgatcct caagcaatat gaagatggct cacccgattg ggaacgcgcc
     2161 aagcgtgttt tcaaccttct cgccgaccgg gttgaaactg tggcgccttg gttggccgag
     2221 caaatcctag ccaatcagag atcaggtgct tgcatccgct ggcttactaa aggcaagctc
     2281 atgcgccgca tgctcttcag cccttttcaa aagcgagact tgtttgaggt ataaaggtcc
     2341 cagcgctccc cgttccgttg cagaaggcgc agacacaatc gtctggctgg ctacattgcc
     2401 agatggcggc ccaagcgggc agtttttccg cgaccaggcg caaatcagat ggtaagttta
     2461 actaaaatga gaaagggttc caacatgttc ctctactatt tctcgggtgg tctattagtc
     2521 ttctcaaccg tgctttatca cgtgacccaa aaaatgactc cccaggatgc gaacccagcc
     2581 ctggccttga ccgtcacgta catgagctcg ctgatcttgt gtatcctttt gttttttctt
     2641 tttccggtga gctcgaactt gagaaccgct ttcagccagt tgaattgggc aagctttggc
     2701 ttggcttttg ccctactcgg cttggaagtg gggttcttac tggcctaccg ggcgggctgg
     2761 aatatcagca tcgcagccat ttttgtcaat gtggctggga tccttctttt ggtgccgatc
     2821 gggcttttct tcttcaagga gagattatct ctcatcaacc tgctgggtat cctggtttgt
     2881 atcgctggcc tgatcatggt gaactggaaa aaatgatcgg ctgtgcagcg cttcaatttt
     2941 gtattcgcta atgataggat aattgagtca gacttaggta ttgctgggat tggtgctacc
     3001 cctcagctca cagacaaagg aggaaaaaca tggctaagat actcgttcat gtcacctacg
     3061 gtccggagca tccgacacga gcagcccttg catttctggt cgccaagagc gcgattgacg
     3121 agggccatac cgtcacgctg tttcttgccg gtgatgctat ccagttgctg cgcgatgcag
     3181 tgcttgacac gctcgcaggc ctgggaacgg gcaagttgcg ggattcgtat gatgcgatcg
     3241 ttgcaggcgg cgggcgcttc tatttatccg gtatgtccag caaggcgcgc gggctcaacg
     3301 aagctgacct ggccggcaag ccggcggagt tcgccatgcc caatgtcctg gtgcgcctct
     3361 cgctggagaa cgaccgcatg tttacctatt aattttcaaa atgaaaccac ttaccaataa
     3421 ggtcgcggtt gttgctggtg caactcgcgg cgctggccgc ggcattgcct gcatgctggg
     3481 tgaagcaggt gccacggtgt attgtacggg gcgcagcacg cgcgccaggt taaaatcgcg
     3541 ccgatctgga aatatggccc catttgccct ggaagggcgt cctgaaacga tcgaagagac
     3601 tgcggagttg gtctcaagat atggcggagt gggcataccg gtccaggtgg accatacaga
     3661 cgaagagcag gttgaacatc tttttgtaca ggtgcagggc gagcaaggca agctggatat
     3721 tttggtcaac gatatttggg gtggggacga ggtttccgag tggggtcagc cgttctggga
     3781 gctctcgatt cagaaggggc tgctgatgca gcagcgcggt gtgcacacgc atatcatcac
     3841 cagtcgctat gcagcaccgc tgatggtcgc acgccgtcgg gggttaatca tcgagattac
     3901 ggatggcgat agtgctgcct accggggcaa tctattctac gatctggcca agtcgtcggt
     3961 gatccgcctc gcctacgcca tggctgaaga gctgcgcccg cacaacatca ctgcgttagc
     4021 cctaacccct ggattccttc gctctgaggc aatgctggaa cattttgggg taaccgaggc
     4081 caactggcag gaaggtgcaa caaaagaccc gcatttcatc gcctccgaga cgccgtatta
     4141 cattgggcgg gcagtggctg cgctggcagc ggacccaaat gtgtttcaga tgtcgggcaa
     4201 ggcgctcagc tcctggaact tggcattgga gtatggcttc aaggatatcg atggtcggca
     4261 acccaattgg ggcaagtatt ttgaagaaca tattgccggc aaaatgtagg tgcagaaaac
     4321 ccccttgtcc tagagcaatc tctaggaaca tagtaattta tacccctgtt gagaataaat
     4381 gatattgtaa agacctagat tacgcttaat ggagagtgca gtgttgaggg gaggaaagcg
     4441 ttttgcttag aaatcttcgt ccagaccgcc cgccccgccg ccctctccgc ctaagtctgg
     4501 tatggctttt tcgatttctt ctggggattg cccagactcc aggcggtcaa tcacatcatc
     4561 aaattcgggg cccatatcct cgcccatctc gcggctcatt ttgcgcatca tgcgccccag
     4621 ggctttagga tcatcttcca ggccagccag gttgcccgga tcagccaggt cttccatgcg
     4681 actctcctca gagcgggcga cgcgcacgcg cccgatgcgc cggcgcacct ggtcgctgtt
     4741 gcagtttggg cagtgcaccg acttggttcc gtattcgcta tagctcataa aggtgtcaaa
     4801 tcgcctgcgg cagttcaggc aatgatattg ataaaacggc atgccgtacc tccattatca
     4861 atgtctttat ttgaattata caaaagttac gcggccagac aggcgctttc ttattcaatc
     4921 gcgtaaatcc tattataatc aggtgtcgag ataaattggt atccagggaa gaaactccca
     4981 cgagttttaa atcagggaag cgaggttagg gaaaacctga gcaaattgta tgactgagcg
     5041 aaatattttt aattcacatc aggatcaacc cctcttgatt gtgatctctg gcccttcagg
     5101 ggcaggcaag gacacggtca tagagcacat gaagcagcgc gagctgccat tctattttgt
     5161 cgtaactgcc acgacgcgcc cgccgcgccc aaacgaagtg cacggtgtgg actatttctt
     5221 cttttcaaag gatgagtttg ctgggatgat cgagcgaggc gaacttatgg aatatgccct
     5281 ggtctacaag gactataaag gtgtgcccaa ggagcaggtc cgccgtgcgc tggccagcaa
     5341 tcaggacgtg gttatgcgcc tggacgtgca gggggctgca accgtgcgca agctggctcc
     5401 ggaggcggtg ctcatcttcc tgactaccag cagcgaggag gagttgatca cacgcctgga
     5461 ggcgcgcatg accgaatcgc ccgaggggct caagctgcgc atcgccaccg cgcgctcgga
     5521 gatgggacgt ctggctgagt ttgactatgt ggtggttaac gcccagaacc aactggacga
     5581 aacggtggat actatcttgg cgatcatcaa ggctgagcac caccgcgtcc tcccccggat
     5641 ggtgaacctg tgaacctgca agacgcaaac acacctgcac ctggtcccga taatcagcca
     5701 ggtcaggaaa ctgcgccagc cactgcaggc gggcagcgat atgtagtggt taagatgcct
     5761 cccaccaagc cctatgtcac gtatgctttt atgggtttta ccatcctggt ctatctgttg
     5821 cagttgggga gcaagttctt cttgggtggg ctagattatc cggcagcgtt gggcttgaaa
     5881 gctaacgacg ccattttgca gggcgagctt tggcgcttgt tcaccccaat gttcctgcat
     5941 tccaccagct ccctgctgca cattggtttt aatatgtatg ctttgtacgc tctggggcca
     6001 gagctggagc gccattttgg gcatcttcgc tatctggctt tgtacctgct aagtggcttt
     6061 gccgggaatg tggcctcctt cctgctctct ccagagtact ccttgggagc gtcgaccgca
     6121 gtttttggcc tcatcggcgc gcaaggagct ttcctgtatc ataatcgtag cctgtttggg
     6181 cgtatcgctc agcgtgccct ggtcaatatt ctggtcgtgg cgggcatcaa cctgttcatc
     6241 gggctctcgc cgggtatcga taactgggga catgtcggcg ggttggtcgg tggtacccta
     6301 tttgcactgt ttggcgggcc gcttctgcag gttgaagcgg aaatgggcgc gctgaagctc
     6361 tccgaccggc gcgagccggg tgcttgggta gcggcagggc tgggggtggg cctgcttttt
     6421 gctgcgctgg cagcggtcaa attctttttg tccggcggat aaatccctta atcaggagga
     6481 gtatgatgag agtccttgtc accggcggag ccggctacat cggcagccat acctgcgttg
     6541 agctgcttca ggcgggctat gaagcggtgg tggtggataa tctgtccaac agcaaagaag
     6601 aggctctgcg ccgggtggag gagatcactg gcaagcggtt ggaatttcac caggtggatt
     6661 tgctcgacga ggctgcgctg gatgccgttt ttcgtcaaaa tccagtggaa gcagtcttgc
     6721 attttgcggg cttcaaagcg gtgggtgaat cggtggctaa gccatggagc tattatcata
     6781 ataatgtcac cggtacgctg gtgttgctta aggtcatgga gcaacacgcg gtcaaggata
     6841 ttgtctttag ctcgtcctgc acagtgtacg gcgatccgca taaggtgccc atcacggaag
     6901 atttccctct ctcggcgacc aacccctacg ggcgcaccaa gttgatcat
//
LOCUS       DBNHOH010000245         2202 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_847150, whole genome shotgun sequence.
ACCESSION   DBNHOH010000245 DBNHOH010000000
VERSION     DBNHOH010000245.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_847150"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="AC16V7_04365"
     CDS             complement(<1..652)
                     /locus_tag="AC16V7_04365"
                     /inference="COORDINATES: protein motif:HMM:NF014713.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HIR6256386.1"
                     /translation="MVISNITLGLAFLAGLASFLSPCVFSLVPAYVGYLGGRTVSSAK
                     SEAPSRWVTFSHGLAFVLGFSVVFILLGVAASALGGVLYDLRTWVAKIGGIVVIVFGL
                     HMTGIFRIPFLEYDLRPQSTPDRRQGYAASALMGVFFSAGWSPCVGPVLGAILTFAIN
                     GGSIAHGVSMLSAYSAGLAIPFLLAALGIGWVTTILRRYGKVMRYAEIVMGVILVIV"
     gene            complement(682..1782)
                     /locus_tag="AC16V7_04370"
     CDS             complement(682..1782)
                     /locus_tag="AC16V7_04370"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HIR6256387.1"
                     /translation="MRKEAITRRNFLHLAALSLGGFALRPLRRIAYLQDFPKYERLGR
                     AFTKVEIKARPDINSQTVGVIYDDAVVPWLREVSGYHPYRYKQRFVETIDGYIWASDL
                     QPVRNLPNQPLQTIHETSLGPGMWAEVSVPWVDIVLENNPASPGFKARLEVGMPLRLY
                     YGQVLWIDKVKSDESGQVWYRVNERYGYGDLIWAAAEAFRPLTHDEVAPISPEVEDKH
                     IVVNVEEKYQTLACYEGKTEVYYCRISGGKKFDPDGTPLGHSSTPTGTHTIWRKQVSA
                     HMSGGTTGAGYDLPGIGWTTLFSGDGVAVHATFWHNNFGGELMSHGCVNATPEDAKWV
                     FRWAHPPVYYDPGDLYSKDTDIPPTKVTVLEG"
     gene            complement(2019..>2202)
                     /locus_tag="AC16V7_04375"
     CDS             complement(2019..>2202)
                     /locus_tag="AC16V7_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005452820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256388.1"
                     /translation="QRTLRTIKQNLFWAFFYNVILIPAAAAGLLNPMLAAGAMAFSSI
                     FVVSNSLRLKGYHTKI"
BASE COUNT          612 a          590 c          508 g          492 t
ORIGIN      
        1 cgacgatcac caggatcacg cccatgacga tctctgcata acgcatcact tttccatagc
       61 ggcgcaggat ggttgtcacc cagccaatgc cgagtgccgc caaaaggaat gggattgcca
      121 gtccagctga ataagccgat agcatactta ccccatgcgc aatcgaacca ccgttgatcg
      181 cgaaggtcaa tatcgcgccg agcacgggac caacgcatgg agaccatccc gccgagaaaa
      241 atacgcccat caacgccgag gctgcatatc cctgtcttcg atctggggtg gattggggtc
      301 gcagatcata ctccaagaac gggatgcgaa aaattccagt catatgcaag ccaaatacga
      361 tcacgactat tccaccaatt ttagccaccc aggtacgcag atcatacaga accccaccca
      421 aggctgatgc cgccacgccc aagagaataa aaactacgct aaaacccagg acaaaagcta
      481 acccgtggct gaatgtcacc cagcgacttg gcgcttcgga ttttgccgat gagaccgtcc
      541 tgccccctaa atacccgaca taggccggaa caagcgaaaa aacacatggt gagagaaatg
      601 atgctaaacc agctaagaac gccagcccga gagtaatgtt cgaaatgacc atattggaaa
      661 ttcaactcct agcaaaatta tttacccctc caaaaccgtc accttggtag gaggaatatc
      721 cgtgtcctta ctataaagat caccagggtc ataataaact ggtggatggg cccaacggaa
      781 aacccatttc gcgtcttcag gggttgcatt cacacaacca tgagacatca gttccccacc
      841 aaagttatta tgccaaaacg tagcatgaac agccacacca tcgcctgaaa agagcgtagt
      901 ccagccgatg ccaggcaaat catacccggc tccggtggtg ccgccactca tgtgggcaga
      961 gacttgcttg cgccagatgg tgtgcgttcc ggttggcgtg gaggagtgcc ccagtggagt
     1021 accatcggga tcaaactttt tgccaccaga tatccgacag tagtacactt cggttttacc
     1081 ctcatagcat gccagggtct gatatttttc ctccacattt acaacgatat gtttatcttc
     1141 aacctcaggg cttattggtg ctacttcgtc atgtgtcaat gggcgaaatg cttctgcagc
     1201 cgcccaaatc aaatcgccat acccgtagcg ctcgttcacc cggtaccata cctgcccgct
     1261 ctcgtcactc ttgaccttat cgatccataa aacctgaccg taatataatc tcaatggcat
     1321 accaacctcc agccgcgcct taaatccagg gctggccggg ttattctcca agacaatatc
     1381 tacccagggc acactcacct ccgcccacat ccccggaccc aggctggttt catgtatcgt
     1441 ttgaaggggc tggttaggaa ggttgcgcac aggctgcaaa tccgacgccc aaatgtaccc
     1501 atcaattgtc tcgacaaagc gctgtttata gcgatatgga tggtacccgg atacctcgcg
     1561 tagccaaggt accaccgcgt cgtcatagat aacaccaacc gtctgcgaat tgatgtctgg
     1621 acgagcctta atctccactt ttgtaaacgc acgccccagc cgctcatatt ttggaaaatc
     1681 ttgcaggtag gcgattcgcc tgagtgggcg cagggcaaac ccacccaagc ttagagcggc
     1741 taaatggaga aaattgcgcc tggttattgc ttctttacgc atggggttaa gcataatcgg
     1801 aaatgttaag agggtcaata gaaggaaatt acaaccacat taataaatga gatagggtca
     1861 ggatagactt tcttacttat caggcgtaat aaattatgga ttggatctat cagcttgttt
     1921 ggatggtctt ctatgtccag ttgactgttg gcaagacata tgcgacgggc agattaaaaa
     1981 tcgatatgcg acgcatccaa ggatacgtcg catatcgatt atatcttggt gtgataaccc
     2041 ttcaagcgca ggctgttgct taccacaaat atactactaa acgccatagc gccggcagcc
     2101 aacatgggat tgagtaggcc agccgctgca gcagggataa gaattacgtt atagaaaaag
     2161 gcccaaaaaa gattttgctt gatggtgcgg agtgtgcgtt gt
//
LOCUS       DBNHOH010000246         2651 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_206111, whole genome shotgun sequence.
ACCESSION   DBNHOH010000246 DBNHOH010000000
VERSION     DBNHOH010000246.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_206111"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="AC16V7_04380"
     CDS             complement(<1..255)
                     /locus_tag="AC16V7_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256389.1"
                     /translation="MPAINLARLRKQTAVLVDHFNQPEAFLRLLHDLLDHYADHILRH
                     GQAGEPPPLIQAYRVPAPMLRQLAQELAPRASEDAPLTLAL"
     gene            complement(263..787)
                     /locus_tag="AC16V7_04385"
     CDS             complement(263..787)
                     /locus_tag="AC16V7_04385"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HIR6256390.1"
                     /translation="MNITVFCSSQTKPGEPAYQEGLRLGQLIGSAGHTVLTGGYIGSM
                     EAVSRGAAEAGGWVVGVTCDEIEAFRPGKANQWVHEERRFSTIRQRLYALIDGCDAAI
                     ALPGGIGTLTEVAMMWSHLQTGVISPRRLVLIGPGWRDSMHTFWRVFDPHVPERFRTL
                     LSFAPDADAAMSTI"
     gene            complement(784..1092)
                     /locus_tag="AC16V7_04390"
     CDS             complement(784..1092)
                     /locus_tag="AC16V7_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016561985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HIR6256391.1"
                     /translation="MELRDLIQRMHEFVSAKGWYETDSPRPQTLRNLAISLNLEASEV
                     LEHFQWGEQVVNNDELAKELADVALYLLQLASLAGIDLEQAILNKLEQNYQRKWDQPR
                     "
     gene            1118..>2651
                     /locus_tag="AC16V7_04395"
     CDS             1118..>2651
                     /locus_tag="AC16V7_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR6256392.1"
                     /translation="MRIILTHEQADFDAIASLLGAYLNDETSVPVLPRRMNRNVRAFL
                     TLYGADLPFVDPRDLPGEPIDFVTLVDTQSLVSVRGMSETTRVHLIDHHPLRDDLPQD
                     WVITVSGTGANTTPFVEMLHEHEHVLTTIQATLLLLGIYEDTGSLTYDRTTPRDLRSA
                     SYLLEQGASLQIAANFLNHPLSKAQQVLYDHLRQTAEYHHIHGYTIIVACGDAREMGE
                     ELSTVAHKLRDLLDPDALCMLVATRGGVQFIARSTNDNIDVAEVAAYFGGGGHERAAA
                     SLIKDRTVEQVHDELVSILPDYVNPAITVAQIMSPDPQLLAPDTPVEEAATRMQRYGY
                     EGYPVVRDGKLIGLLTRRAVDRAISHKLKVNAASLMEAGEFTVQPDDSIEHLQRLMTD
                     TGWGQIPVIDPQTGEIIGIVTRTDLIKTLSPRPRIPGRQNLAERLERALPPARLALLK
                     SVAKVAHEQKVALYIVGGFVRDLLLERPSLDFDLVVEGDAIALARALSRKHGGRVTSH
                     ARF"
BASE COUNT          562 a          727 c          759 g          603 t
ORIGIN      
        1 gagtgccagg gttagcggtg catcttcgga ggcacgcggc gctagttcct gggctaattg
       61 ccgtaacatt ggggctggaa ctcgataggc ctggatgagt ggcgggggct cacctgcctg
      121 tccatgacgc aggatgtggt cggcgtaatg atccaacagg tcgtgcaaga ggcgtaaaaa
      181 agcctcgggt tggttgaaat gatctaccag gacggcggtt tgttttcgca gccgggctaa
      241 gtttatggca ggcatagaat aactatatcg tgctcatagc tgcatcggcg tctggggcaa
      301 aggagagcag cgttcggaat ctttccggta catggggatc gaaaactcgc caaaatgtgt
      361 gcatggaatc gcgccagcca ggcccgataa gcaccagacg acgcggggag attaccccgg
      421 tttgcaggtg cgaccacatc atggctacct cggtcagggt gcctatacca ccaggcagcg
      481 caatggctgc atcgcagcca tcgatgaggg cataaaggcg ctgccgtatg gtggaaaagc
      541 gacgctcttc gtgtacccac tggttggctt tgcctggacg gaaggcttcg atctcatcac
      601 aggtgacgcc gaccacccag cccccagcct ccgctgcccc gcgcgagacg gcttccattg
      661 agccgatgta ccccccggtg agcaccgtgt gacctgcgga gccgatcagt tgtcccaggc
      721 gcaacccttc ctgataagct ggttctcctg gtttggtttg tgagctgcaa aaaacggtga
      781 tgttcatctc ggctgatccc atttccgctg gtagttttgt tccagcttgt ttagtatggc
      841 ctgctccaga tcgatgccag ccaggctggc gagttgtagc aggtaaaggg ctacgtcggc
      901 gagctcctta gccagctcat cattattcac cacttgctca ccccactgga aatgctctag
      961 aacctcggag gcttccagat tgagagagat ggccaggttg cgcagggttt gtggccgcgg
     1021 gctgtctgtt tcataccacc cttttgcgga cacaaattcg tgcattcgct ggataaggtc
     1081 gcgaagttcc ataggtgttg attataatac atacagtatg cgtatcatcc tgacacacga
     1141 gcaagctgat tttgacgcta tcgcctcatt actgggggcg tacctgaatg acgagacctc
     1201 tgtcccggtt ctgccacgcc gcatgaaccg caatgtccgc gcctttctga ccctatatgg
     1261 cgcagacttg ccttttgtcg acccccgcga tctgccgggt gagccgatcg atttcgtgac
     1321 cctggttgac acgcaatccc tggtcagcgt gcgggggatg agcgaaacca cccgcgtgca
     1381 cctgattgac caccatccat tgcgggacga cctgcctcag gattgggtga tcactgtttc
     1441 gggtacggga gcgaacacta ccccatttgt cgagatgctg catgagcatg aacacgtgct
     1501 gaccacaatc caggctacct tgctcctgct agggatctat gaggacactg gatcgctaac
     1561 ttacgaccgg actacgccac gtgatctgcg ctccgcaagc tatttgctgg aacaaggtgc
     1621 cagcctgcag attgctgcta attttctcaa ccatcccctg tccaaagcgc agcaggtgtt
     1681 gtatgaccat ctgcgccaga ccgcggagta tcaccacatc cacggatata cgatcattgt
     1741 ggcgtgtgga gatgcccgtg agatggggga ggagctatct acagttgctc ataaattgcg
     1801 cgatttgctg gaccccgatg ccttgtgcat gttggttgcc acgcggggcg gggtgcagtt
     1861 catcgcccgc tctaccaacg acaatataga tgtcgccgaa gtggcagcct actttggagg
     1921 cggaggtcat gagcgggcgg ctgccagctt aatcaaagac cgcactgttg aacaagtcca
     1981 cgacgaattg gtgagtatcc tgccagatta tgtcaacccg gcaattacgg tcgcccaaat
     2041 tatgtctcct gacccgcaat tgctcgctcc agacactcca gtagaagagg ctgccacccg
     2101 gatgcagcgc tatggttatg aaggctatcc tgtggtgcgc gatgggaaat tgattggatt
     2161 gctgacgcgt cgggcagttg atcgcgccat ttcccataaa ctcaaggtaa acgcagcgag
     2221 cttgatggaa gctggcgagt tcaccgttca gcctgatgat tcaattgagc atttgcagcg
     2281 cttgatgacc gacactggct gggggcaaat cccggtgata gatccacaga ctggagaaat
     2341 cattggaatt gtcacccgta ccgacctgat caagaccctc tcgccacgcc cacgaattcc
     2401 tgggcgccaa aacttagctg agcgcttgga aagggctctg cccccagcgc gtctcgcctt
     2461 gctaaagtcg gtcgctaagg tagctcatga gcagaaagtc gccttgtata ttgttggagg
     2521 gttcgtccgc gatctgttat tagaacgccc cagcctggat tttgacctgg tggtcgaagg
     2581 tgatgcgata gccttggcgc gcgcattatc ccgcaagcat ggcgggcgcg tcacaagcca
     2641 cgcgcgcttt g
//
LOCUS       DBNHOH010000247         1694 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_78095, whole genome shotgun sequence.
ACCESSION   DBNHOH010000247 DBNHOH010000000
VERSION     DBNHOH010000247.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_78095"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="AC16V7_04400"
     CDS             <1..207
                     /locus_tag="AC16V7_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HIR6256393.1"
                     /translation="SLEQDADIVMFIHRPEVYEKDTLKQNIAQIKVAKHRNGPVGMVE
                     LIFRHQLAKFENAATRVIDLTKVA"
     gene            234..950
                     /locus_tag="AC16V7_04405"
     CDS             234..950
                     /locus_tag="AC16V7_04405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HIR6256394.1"
                     /translation="MNSSFKGFPQGKLRLTPIPALFFHELLPYIDHLGELKVSLYAFW
                     RMSYQEGNFRYLQLGDFTGDERFMQGLGGTPEAAQAALHDSLERAVQRGTLLRASIEV
                     EEGEQSLYFVNSPRGREALQAIARGEWRPSGQAQQPVEILDEAPNIFRLYEENIGPLT
                     PMIADALREAEQEYPIQWLEDAMRIAVENNARNWRYVEAILERWQDKGRDEQKDRRDT
                     EKARRKYAEWENLGSGQDRS"
     gene            862..>1694
                     /locus_tag="AC16V7_04410"
     CDS             862..>1694
                     /locus_tag="AC16V7_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012529.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6256395.1"
                     /translation="MSKKIDETLKKLAASMQSGKTSAPDKTEAERNSSSSDLPGDPNC
                     PHCGGLGYLRQDLPPGHPDFGKLQVCSCRNSQVSQQVRQRLFALSNLDELTHLSFENF
                     QPRGRVGLWPQEADSLEQAHNQARQFAQSLNGWVVFQGGYGCGKTHLAAAIANFAVGM
                     GVPTLFITVPDLLDSLRYTYDNPQATFEERFEEIRRAPLLILDDFGTQNATAWSQEKL
                     FQIFNYRYINRLPLVVTTNTPLEDIEGRIRSRLEDPDLVTKVQILAPDFRRPAGDTGQ
                     N"
BASE COUNT          403 a          504 c          460 g          327 t
ORIGIN      
        1 tcactggaac aagacgcgga tatcgttatg tttattcacc ggcccgaggt gtatgagaaa
       61 gacaccctca agcaaaacat cgcccaaatc aaggttgcca agcaccgtaa tggtccggtt
      121 ggcatggtgg aactgatctt ccgccaccaa ttagcaaaat ttgagaatgc tgccacacgg
      181 gtcatcgatc tcactaaagt ggcatagcgt tatagttgta aaatacaagt gcaatgaact
      241 cttcttttaa aggctttcca cagggaaaac tacgcctcac acctatccca gcactcttct
      301 tccatgagct cctgccatat atcgaccacc ttggcgagct aaaggtgagc ctgtacgcct
      361 tctggcggat gagctaccag gaggggaatt tccgctacct gcaattgggc gatttcacag
      421 gggacgagcg tttcatgcag gggctgggag gcacacccga agcggcgcag gcagcgctgc
      481 acgactcgct ggagcgggcg gtgcagcgcg gtacgttgct gcgggcatcc attgaagtag
      541 aagagggtga gcagtccctg tacttcgtga actcaccgcg cggacgggaa gccttgcaag
      601 ccattgcacg cggcgaatgg cgcccatccg ggcaagcgca acaaccggtc gagattctcg
      661 acgaggcccc caatattttt cgcctatacg aagaaaatat cgggccgctt acgccgatga
      721 ttgccgatgc cctgcgggaa gccgaacaag aataccccat ccaatggctt gaagatgcca
      781 tgcgcattgc tgtagaaaac aatgcccgca attggcgcta cgttgaggcc atcctggaac
      841 gctggcaaga taaaggtaga gatgagcaaa aagatcgacg agacactgaa aaagctcgcc
      901 gcaagtatgc agagtgggaa aacctcggct ccggacaaga cagaagctga gagaaactct
      961 tcttcgagcg atttgcctgg cgacccaaac tgcccgcact gcggcgggct cggctatttg
     1021 cgccaggact tgcccccagg acatcccgat ttcggcaaac tgcaggtatg ttcctgccgt
     1081 aacagccaag tgagccagca ggtgcgccag cgcctgttcg ccctcagcaa tctagacgag
     1141 ctgacccacc tgagcttcga gaacttccag ccgcgcggcc gggtggggct atggccccaa
     1201 gaagccgatt cgctggagca ggcccataac caggcgcgcc agtttgcaca gtcgctcaac
     1261 ggctgggtgg tttttcaggg tggctacggc tgtggcaaga cccacctggc ggcagccatc
     1321 gccaacttcg cggttggcat gggcgtgcct accctgttca tcaccgtgcc cgacctgctg
     1381 gactccctgc gttataccta cgataatcca caggcaacct tcgaagagcg cttcgaggag
     1441 atccgccgcg cccctttgct catcctggac gactttggca cccagaacgc caccgcctgg
     1501 tcgcaggaaa aactcttcca gatcttcaat taccgctaca ttaaccgcct gcccctggta
     1561 gtgaccacca acacccccct ggaagacatc gagggtcgca tccgctctcg cctggaggat
     1621 ccagacctgg tcacgaaagt gcaaattcta gcccctgatt tccgccgtcc agctggggat
     1681 accggccaga atag
//
LOCUS       DBNHOH010000248         3435 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_269964, whole genome shotgun sequence.
ACCESSION   DBNHOH010000248 DBNHOH010000000
VERSION     DBNHOH010000248.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_269964"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..643)
                     /locus_tag="AC16V7_04415"
     CDS             complement(128..643)
                     /locus_tag="AC16V7_04415"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR6256396.1"
                     /translation="MVDNTQNSPRLVRELMTVGVETCSPDTLVVDLARVFLEKGIEEI
                     VVLDDGNALGVVGQEELIRAYARGDFESLKAADVLREGVLQVPPDIPLAAAAEIMLDH
                     GVRSLFLMHHAGGIEYPAAYISYRHYLRYIAAQEIDDLQDLGIYAQRSSPLETFFQKR
                     DKLRRPGSSAK"
     gene            complement(726..3020)
                     /gene="acsB"
                     /locus_tag="AC16V7_04420"
     CDS             complement(726..3020)
                     /gene="acsB"
                     /locus_tag="AC16V7_04420"
                     /EC_number="2.3.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit alpha/beta"
                     /protein_id="HIR6256397.1"
                     /translation="MSRYIATRAIRGANALVSEAELMLQKAIREKGIETPVSFPNTAY
                     FLPLTLGMTGVAVENLGDLKPVLDHARNLLHPTPSVKHWTPYLGETLDSGMATLLAAE
                     TIEAVRFVYNLQPEAMPGFQLAGGTAFTSPDAGANGGGNGMDGHLNGPIDDIQLRSWG
                     IQLVDGRMPGFAAIVGCAKSNEVAVKIVRELQRRNILCFLSGNVNGRSVIHQLNEEGV
                     ELGYDTYTVPFGTDTISAIYALGFATRSALTFGGLKPGQARDILLYNRERVFAFVLAL
                     GEVDDLKYAAAAGAINFGFPVIADTVIPEILPTGITRYEHVVSMPFNEIEGKDDLERA
                     ERLVQKCIEVRGVKVKVADVPVPVPYGSAFEGEVVRKNDMRVEFGGKRSRCFEYLHMA
                     PLEEVVDGKVEVVGADFENVPAQGSMDVGIVVKVAGRQMQEDFEPVLERQIHYFINGA
                     SGIQHIGQRDIAWIRISANAADKGFNLEHFGKILHARFHSEFGAIVDKVQVTIFTEPE
                     QMQEWLAKARQAYDHRNKRLADLTDDKVEEFYSCTLCQSFAPNHVCVVSPQRLGLCGA
                     YNWLDCKASFSINPTGPNQPIKLGKLIDMRKGYWEGTNDYAKIGSHGVVEEVSMYSIM
                     ENPMTACGCFECIVMLIPEANGVMVVSREDTSMTPAGMTFSTLAGIAGGGLQTPGVMG
                     VGKYYLISPKFISADGGFKRVVWMSSVLKESMAEELEAVAEREGDPDLINKIADDRNA
                     STVDELLAWLEEQNHPALVMDPIF"
     gene            complement(3095..>3435)
                     /locus_tag="AC16V7_04425"
     CDS             complement(3095..>3435)
                     /locus_tag="AC16V7_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256398.1"
                     /translation="IATYVVASGGYVIFGGSSPVSGMPDRMSDSDIILRYISEGWEKI
                     YGGKLEFIKEADEMIRRTLEHIDKKRAALGLPEYNPDNFGRSGDWRMLEVENLPLSER
                     RAAIYGVAAD"
BASE COUNT          852 a          955 c          779 g          849 t
ORIGIN      
        1 ccctcggact gcatcgcacc aatcgtgatg gcgcgaacag aaccaggcca tttatctatg
       61 ggaatttcga tcggcatgta tttcactcct atgtcgttta gaatcgactt attaacggca
      121 atcctaatta ttttgcggaa ctgcctggtc tgcgtagctt gtcccgtttt tggaagaatg
      181 tctcaagcgg agagctacgc tgagcataga ttcctaaatc ctggagatca tcaatttctt
      241 gagctgcgat ataacgcagg taatgacggt aagaaatata agccgcagga tattcaattc
      301 caccggcatg atgcatcaag aacaatgagc gcacaccatg gtcaagcata atctcggccg
      361 cggctgccaa cggaatatct ggaggcactt gcagcacccc ttcacggagc acgtccgcag
      421 ctttgagaga ctcaaagtct cctcgggcgt aagcgcgtat aagctcctct tgacctacaa
      481 caccaagggc attgccatca tctaagacaa caatctcttc tatgcctttt tctaaaaaga
      541 cgcgcgccag atcaacaacc agggtatctg gtgaacatgt ctccacgccc acagtcatta
      601 actcgcggac cagacgggga gaattttgtg tattatctac cacaagtttt atttagaggg
      661 gaaagggata gggtgaatgc gacgacgata aattgatgtt accctatccc ttattcccat
      721 ctcctttaga atattggatc cattacgagc gctggatggt tttgttcttc caaccatgcc
      781 agcagttcat ccaccgtaga tgcattgcga tcatctgcga ttttattgat gaggtctgga
      841 tcgccctcac gctcagcgac agcttcaagc tcctccgcca tgctctcctt caacacgctg
      901 ctcatccaaa ccacgcgttt gaagccacca tccgctgaga taaacttggg gctgatcaag
      961 taatacttgc caacacccat cacacccggg gtttgcaagc cgccacctgc gatgccagcc
     1021 aaggtgctga aggtcatacc agccggggtc atgctggtat cttcacggct aaccaccata
     1081 acaccatttg cttccggtat aagcataaca atgcactcaa aacatccaca agcagtcatg
     1141 gggttctcca tgatcgagta cattgagact tcctcgacca caccgtgtga accgatcttg
     1201 gcgtaatcgt tggtaccctc ccagtatccc ttgcgcatat caatgagttt tccaagctta
     1261 atgggctggt tgggtcctgt tggattgatg ctgaaagatg ctttgcaatc cagccagtta
     1321 tacgcaccgc ataaacccag tctttgtggg ctgaccacac acacatggtt cggcgcgaag
     1381 gactggcaaa gcgtgcaaga gtagaactct tccactttat catcggtcag atccgccaag
     1441 cgcttgttac gatggtcgta tgcttggcga gcttttgcta gccactcttg catctgctcc
     1501 ggttcagtga aaattgtgac ctgcacctta tccacgatag caccaaactc agagtggaat
     1561 cgggcgtgca ggatttttcc aaaatgttcc aggttgaaac ccttatcagc cgcgttcgcg
     1621 gatatacgga tccaagcaat atctcgctgg ccaatgtgct gaattcccga ggcgccattg
     1681 ataaagtagt ggatttggcg ctcgagaact ggttcaaagt cctcctgcat ttgcctacca
     1741 gctaccttta ccacaatacc cacgtccata ctaccttgtg ctggcacgtt ttcaaagtcc
     1801 gctcccacca cctctacttt cccatcgacg acctcttcta gcggtgccat gtgcagatat
     1861 tcaaagcagc gagagcgttt tcctccaaat tccacccgca tatcgttctt gcgcaccact
     1921 tcaccttcga aggcagagcc atatggtact ggcacgggca catcggcgac tttgaccttc
     1981 acgccgcgca cttcgatgca cttttgcact aggcgctcag cacgctccag atcatctttt
     2041 ccttcgatct cgttgaaggg catgctgact acatgctcgt aacgggtaat tcctgtgggc
     2101 aagatttctg ggatgactgt gtctgcgatg accgggaagc caaaattgat cgctccggcc
     2161 gctgctgcgt actttagatc gtcaacttcg cctaaagcaa gcacaaaggc gaagacgcgc
     2221 tcacgattgt acaagagaat atcgcgtgct tgaccaggct ttaagccgcc aaaggtcaat
     2281 gccgacctgg tagcaaaacc taacgcatag atcgcactga tggtgtcggt gccaaatgga
     2341 acggtatagg tatcatatcc cagctcgaca ccctcttcgt tcaattggtg aatcacgctc
     2401 cgaccattaa cattgccaga aaggaagcac aaaatattgc ggcgctgaag ctctcgaacg
     2461 atcttgaccg ccacctcgtt cgatttggca catccaacaa tggctgcaaa tccgggcatg
     2521 cgtccatcaa ctagctgaat accccaagat cgcagctgta tatcgtcgat gggaccgttg
     2581 aggtgcccat ccattccatt acccccacca ttagcaccag catccgggct ggtgaaggca
     2641 gtcccaccag ctagctggaa gccaggcatg gcttcaggct gaaggttata aacaaaacgc
     2701 acagcttcga tcgtttcggc tgccagcagc gtcgccattc cactgtccaa tgtttcaccc
     2761 agataaggtg tccagtgctt gactgacggc gtgggatgca ataggttacg cgcgtgatcg
     2821 agaacaggct taagatcgcc cagattctct acagccaccc cggtcatccc taaggtcaaa
     2881 gggagaaaat aagctgtatt tgggaaagaa actggtgtct caattccttt ctcacgaata
     2941 gccttttgga gcattaactc cgcttcagaa actaacgcat tcgcaccacg gattgcgcgg
     3001 gttgcaatgt atcgtgacat ttttcctccg agtcgctatc gtattgtgga catttttctc
     3061 atattcagtg aagccgcaca atacaggctg tttctcaatc ggccgcaaca ccatagattg
     3121 ctgcgcggcg ttcggagagt ggcaaattct ccacctccag cattcgccag tctccactac
     3181 gcccaaagtt atccggattg tattcgggca acccaagcgc agctcgcttc ttgtcgatat
     3241 gctcaagtgt gcggcggatc atttcatccg cttccttgat aaattcaagc ttgccgccgt
     3301 aaatcttctc ccagccttcg ctgatgtatc gcaagataat atcgctatct gacatacggt
     3361 caggcattcc gctcactggg gacgaaccac cgaatatcac ataacctcca gatgcaacaa
     3421 cgtaggtggc gatag
//
LOCUS       DBNHOH010000249         2717 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_270071, whole genome shotgun sequence.
ACCESSION   DBNHOH010000249 DBNHOH010000000
VERSION     DBNHOH010000249.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_270071"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1230
                     /locus_tag="AC16V7_04430"
     CDS             <1..1230
                     /locus_tag="AC16V7_04430"
                     /inference="COORDINATES: protein motif:HMM:NF025104.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR6256399.1"
                     /translation="PPITSCDDDRITPIGRWLRDTKLNELPQLWNVLNGDMSLVGPRP
                     EDPEIFLTLPENYREKIVSVRPGITSPASIMYHDEEKILTSKNLMDDYAELILPNKLR
                     MDLLYVRYHNLINDFDTLFWTFAILIFRIGKQNIREGWLYNGPIARFVRHYLNWFMID
                     FITASAGIVSVGLGWRLFEPLDLGISNALILGIILAFSFSISNSILEVNLISWQQAGA
                     GDSIKLIISCLLVGIFYIILNVYVGIIPQLTTLFIAIACLVVLGAFVGVRYRSRLIMG
                     IATRWINSRQSSIGVYERALIIGAGNAGKFTTWTLKHPDFRHFYKVIGYVDDAPGKQG
                     MRFDGIKVLGTTADIPNLVTKHNIGLLFYSIRDANETGKARILSICRQVELPIVSVPD
                     VIDNMLHLFAQSMKSID"
     gene            1262..1492
                     /locus_tag="AC16V7_04435"
     CDS             1262..1492
                     /locus_tag="AC16V7_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256400.1"
                     /translation="MSFDWKNITFLATDAGSLFGSHFVETLVNRGESAPGAEREVTLP
                     LYALMAEVDVRAVVSAAREALGESGLNQLDFM"
     gene            1493..2365
                     /locus_tag="AC16V7_04440"
     CDS             1493..2365
                     /locus_tag="AC16V7_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013633343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6256401.1"
                     /translation="MTDTLLNQFDDSDWSLIIEPRRKLLNLHLRELWRYRDLVLLFVR
                     RDFVSVYKQTILGPLWYLIQPLLTTLTFTVIFGNIAGLPTDGLPQFLFYMSGTVIWAY
                     FADCLNKTANTFAGNASLFGKVYFPRLAVPVSILISNLITFFFQFALFLLFVIFFAVR
                     GAPIQLHWGWVALSPLLVLMMAGLGLGFGIIISSLTTKYRDLRFLVSFGVQLFMYATP
                     VIYPASSIPARFKWIVQANPMTPVVESFRYAFLGNGTVSYFQLLYSFAFMLVVVFLGS
                     LVFNRVEQTFMDTV"
     gene            2490..>2717
                     /locus_tag="AC16V7_04445"
     CDS             2490..>2717
                     /locus_tag="AC16V7_04445"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR6256402.1"
                     /translation="MNPFWKDKTVLVTGAGGFIGSHLVEALLARDAKVRAYVRYTSRR
                     DPGLLRDLSPADFARLEVVSGDLRDERAVRES"
BASE COUNT          577 a          677 c          700 g          763 t
ORIGIN      
        1 ccacctatta cctcctgcga tgacgaccgc attaccccca ttggtcgttg gctgcgagat
       61 accaaattga acgaacttcc gcaactttgg aatgtattaa atggtgatat gagcctggta
      121 gggccccggc ctgaagaccc ggaaatcttt cttacactgc ctgagaatta tcgcgaaaaa
      181 atagtatctg ttcgccccgg aattactagt ccggcgagca ttatgtatca cgatgaagag
      241 aaaattttaa cttcaaagaa tttgatggat gattatgcag aattaatttt acccaataaa
      301 ctgcgcatgg acctgttgta cgttcgatac cataatctta tcaatgattt cgacaccctt
      361 ttctggacat ttgccattct tatcttccgg attggcaaac aaaatattcg ggaaggctgg
      421 ttatataacg gtccgattgc ccggtttgtg cgtcactatt taaactggtt tatgatcgat
      481 tttataaccg cttcggcagg aattgtttcg gtagggcttg gatggcggtt gtttgagccc
      541 ctggatttgg gaatatcaaa cgccctcatc cttgggatca tcctggcatt ttcattcagc
      601 attagcaatt ctatattgga agtaaatctg atttcctggc agcaggcagg ggctggtgat
      661 tcaatcaagt tgattatttc ttgcctgctt gttggcattt tttacatcat tcttaatgta
      721 tatgtcggga ttattcccca gcttaccact ctcttcattg caattgcttg cctggtagtt
      781 ctcggagcat ttgtaggggt acgctaccgg tcgcgcttga tcatgggcat tgccacacgc
      841 tggattaatt ctcgtcaaag cagtatcggg gtatatgaaa gggcccttat cataggagct
      901 gggaacgcgg gcaaatttac gacctggacg ttgaagcacc cggatttccg gcatttctat
      961 aaggtgatcg gttatgtgga cgacgctccc gggaaacagg gtatgcgatt tgatgggatc
     1021 aaggtattag ggacaactgc agatatccca aatctggtta cgaagcacaa catagggttg
     1081 cttttctatt cgattcgaga tgcaaatgaa acgggtaagg ctcgcattct ttcgatctgc
     1141 aggcaggtgg aattgccgat tgtatctgtt ccagatgtga tcgataacat gctccatttg
     1201 ttcgctcaaa gcatgaagtc tattgattaa aactttcagc cggatatcag gtttcatata
     1261 tatgtcattt gattggaaaa atataacttt tctcgcaacc gacgcaggca gtcttttcgg
     1321 cagccacttc gtcgagacgt tggttaaccg tggagaaagc gctccaggcg ctgaacgcga
     1381 ggtgaccttg cccttgtacg ccctgatggc agaagtcgat gtgcgagcgg tggtgagcgc
     1441 cgcgcgcgag gcgctgggcg agagtggctt aaaccaattg gatttcatgt agatgacgga
     1501 cacccttctc aaccaatttg atgatagtga ctggtcgctg atcattgagc cgcggcgcaa
     1561 gctgctgaac ctgcacctga gggaactgtg gcgctaccgc gacttggtgc tgctgtttgt
     1621 gcgccgtgac tttgtgtctg tctacaaaca gaccatcctt ggcccgctgt ggtacctgat
     1681 ccagccgttg ctcactaccc tcaccttcac cgtgatcttc ggcaatatcg ccgggctccc
     1741 cacggacggc ctgccgcagt tcctgttcta tatgtctggc acggtcatct gggcttactt
     1801 cgccgattgc ctcaacaaaa ccgccaatac ttttgccggc aatgccagcc tgtttggcaa
     1861 ggtttacttc ccacggttgg cagtgccggt ttctatcctg atctccaacc tgatcacctt
     1921 cttcttccag tttgctctgt tcctgctgtt cgtgatcttt tttgcagtgc gcggcgcgcc
     1981 tatccagcta cactggggct gggtggcgct ctcccccctg ctggtgctga tgatggccgg
     2041 gctgggcttg ggcttcggca tcattatttc atcgcttacc accaagtacc gtgacctgcg
     2101 tttcctggtc tcttttggtg tgcagctgtt catgtatgcc acaccggtca tttacccggc
     2161 ctcatctatc ccggctcgtt tcaaatggat cgttcaggcc aatccgatga cccctgtcgt
     2221 ggagtcattc cgttacgctt tcttgggcaa cggtacggtg agctacttcc agctgctgta
     2281 cagcttcgcc ttcatgctgg tggtggtctt tttgggcagc ttggtcttca accgggtgga
     2341 acagacgttc atggatacgg tgtgaagact ttggccatta gaagaaattc gaagtgttca
     2401 aagaactatc tcaaaagact tcccagcggt ttgaggtcag gtggcaaaat cgttatccaa
     2461 tcatgtcgga tgatacgagg ggacatacta tgaacccctt ctggaaagat aaaaccgtgc
     2521 tggtcaccgg cgccggcggc ttcatcggca gtcacctggt cgaggccctg ctggcccggg
     2581 atgcgaaggt gcgtgcctat gtgcgctata cctcgcgcag ggatcccggc ctgctgcgcg
     2641 acctgagccc tgccgacttt gccagattgg aggtggtttc gggcgacctg cgggacgagc
     2701 gcgctgtccg cgaatcc
//
LOCUS       DBNHOH010000250         2107 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1040119, whole genome shotgun sequence.
ACCESSION   DBNHOH010000250 DBNHOH010000000
VERSION     DBNHOH010000250.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1040119"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..374
                     /locus_tag="AC16V7_04450"
                     /pseudo
     CDS             198..374
                     /locus_tag="AC16V7_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012576.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
     gene            402..1295
                     /locus_tag="AC16V7_04455"
     CDS             402..1295
                     /locus_tag="AC16V7_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR6256403.1"
                     /translation="MIRQRSYTLGVVTAGLKYIGPSRTLSGITRAAEGVGYSLLLKEL
                     PHFDANDVIPIFQTLLSRHVDGIIWAVPEVGDNRNWVSKLSLELEIPIVYLAMNSQEN
                     IYVVSINNYLGGQLAISHLLEQGYRHIGHISGPLDWWEARERMAAWRDVLRAADLEAK
                     DEHWAEGNWSSASGAQATERLFNQYPEMDAIFVANDQMALSVMQYACRKGLRIPDDLG
                     IVGFDDIPESAFFWPPLTTVQQNQYNVGEVVVEEIIKIIESYRQEQEPVQPKSIMLAP
                     ILIVRQSSLRMRGVKGGESGR"
     gene            1496..>2107
                     /locus_tag="AC16V7_04460"
     CDS             1496..>2107
                     /locus_tag="AC16V7_04460"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR6256404.1"
                     /translation="MRVVRLHSQGELRLHDEPEPVPLAGEELLRVKAVGVCGSDLHWF
                     TEGEIGDATLDSPLVLGHEFSAVTGRGERVAVDPAISCGLCEFCQKGYPNLCPQVKFA
                     GHGLQDGALREYMPWPQDHLYRLPDVLSDAEGAMLEPLGVALHATDLGKLRAGMSVGV
                     FGCGPIGLLVVQLARLSGAVQIIATDKLAHRVEAARNYGAHQAI"
BASE COUNT          524 a          453 c          554 g          576 t
ORIGIN      
        1 cgcccaaact cccccatcac ctctcgaact gcgtaactta tgagatattt accattagac
       61 ggagtttaga aaaagcgctg gtcaaccact ggcgaggttg aattgtttaa tctggacctg
      121 attatgaatt gttgggtttc ccctatgatt aaatatatga tatttgtctc ttgatttcct
      181 atttcaattt tgctataatg ttatcgataa cgggatcgat aacattgtat cagcgaactg
      241 gacgaccaac cattaaggaa gttgcgtctg cagcgggggt gtctacgcag acggtatccc
      301 gcgtgattaa caataggcca gacgtttccc cccaaactcg aagacgcgtt caagaaatca
      361 tagatcagtt aggctatcaa cccagtgccc tggctcgtag cttgatccgc cagcgcagct
      421 acactttggg ggtcgttact gccggcttga agtatatagg cccatcgcgt actttgagtg
      481 gcatcacccg tgcggcagaa ggagttggtt actcattgtt attaaaagag ctcccgcatt
      541 tcgatgccaa tgacgttatt cctatttttc aaacgttgct ttctcgccat gtggacggga
      601 ttatctgggc tgtccccgaa gttggggata atcgaaattg ggtcagtaag ctatctctgg
      661 aattagaaat cccaatcgtc tacctggcta tgaattcaca agaaaatatc tatgtagtat
      721 ctatcaacaa ttatcttggt ggacaattag caatctccca tttgctggaa caaggatatc
      781 gacacatcgg gcatatctca ggtccgttag attggtggga agctcgtgag cgtatggcgg
      841 catggagaga tgttttgagg gcagctgatc ttgaagccaa ggatgaacat tgggcggagg
      901 gaaattggtc ttcggcaagc ggcgcgcaag caaccgaacg acttttcaat caataccctg
      961 agatggatgc gatttttgtc gccaatgacc agatggctct aagcgtgatg caatacgctt
     1021 gtcgaaaagg cttgcggatt ccggatgatc taggaattgt cggttttgat gacattcccg
     1081 aatcggcctt tttttggccc ccattgacaa cagtacagca gaatcaatat aacgtcggtg
     1141 aagtagtcgt ggaagaaatt attaaaatca tagagtctta ccggcaagaa caagagccag
     1201 ttcagcccaa gtctattatg ttggcaccga ttttaattgt acggcaaagt tcattaagaa
     1261 tgaggggggt caaaggaggt gaatctggga gatagtctca tctcccattg cttaagcctg
     1321 ctgaatgata tctttttcga ttggtttgtc tcggttgatg aaatttcaac tgttgattac
     1381 aagactattt tggctggaac cttaggtcat aaaaacacca gtattacggt tgtcggtcgc
     1441 gtctttgtga cgcaccccag tattagcgag gattttttca agagtaacaa acgtgatgag
     1501 agtcgtgcgc ctccattccc agggagaact ccgcttgcat gatgagcccg agccagtacc
     1561 ccttgctggt gaggaattgt tgcgagttaa agcagtaggc gtatgtggat cagatttgca
     1621 ctggttcacc gaaggggaga tcggtgatgc tacattagat tcccctttgg ttttggggca
     1681 tgagttttca gcggtcactg gcagaggcga gcgtgtcgcc gttgacccag ctatctcatg
     1741 cgggctgtgc gaattttgcc aaaagggata tccaaacctt tgccctcaag tgaaatttgc
     1801 aggacacggc ttacaggacg gcgctctgcg cgaatacatg ccatggccgc aggatcatct
     1861 gtatcgccta ccagatgtat tgagcgatgc tgaaggcgcc atgttggagc ctctcggtgt
     1921 agctttacat gcaaccgatt tgggtaaatt acgagctggg atgagcgtag gcgtgtttgg
     1981 atgcggaccg attggtttgc ttgtcgtgca gttggcgagg ctttcggggg cagtgcagat
     2041 tattgctacc gataagctcg cacatcgcgt ggaggctgcc aggaactatg gggcgcatca
     2101 ggcgatt
//
LOCUS       DBNHOH010000251         1695 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_14082, whole genome shotgun sequence.
ACCESSION   DBNHOH010000251 DBNHOH010000000
VERSION     DBNHOH010000251.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_14082"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..912
                     /gene="rlmB"
                     /locus_tag="AC16V7_04465"
     CDS             175..912
                     /gene="rlmB"
                     /locus_tag="AC16V7_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIR6256405.1"
                     /translation="MREWINGRNPVYEVLRAARRQVFRLQIAQGVQEKGRLAEIASLC
                     QRGRIPIERPQRNQLDARFPNNQGVALEVSSYPYSDLVDMLALAEKRKEPPLLLILDT
                     LQDPQNLGTLLRTAEIVGVHGVLLPLRHTATVTPAVVHASSGASEHLLITQLNLAQAI
                     ETLKREDVWVVGLEGDPAEAVPSDVRLDGPLALVVGSEGEGMRSLVRKSCDVLLRLPM
                     RGHVESLNAAVAGSVALYLAWQARRWG"
     gene            complement(1005..>1695)
                     /locus_tag="AC16V7_04470"
     CDS             complement(1005..>1695)
                     /locus_tag="AC16V7_04470"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferredoxin--NADP reductase domain-containing
                     protein"
                     /protein_id="HIR6256406.1"
                     /translation="VGQDIQAAKDFILSALPKAYESVSESRLLFDFLASPVRILGDAS
                     GQVQGLEVEETKLIPFNGDTKARGTGIKRNLDVDTVIFCIGDKVDETFGLPVQWNEFV
                     KNPNPSYPVNGISHEVFDAQTGQPIQGIFVAGWSREASAGLVGVARKDGESGAQAMLQ
                     YLQTMPAMENPTAVADNVNKLLQKISKPIITKANILRLEAVEQSEAERRNLDEFKFGS
                     NEEMLAAIGSG"
BASE COUNT          367 a          437 c          488 g          403 t
ORIGIN      
        1 tggaagatgg gcgcggtcaa gtaaacagta aagcgacggt ggtggtcacc aaggttttgc
       61 agacggcagc tggtcgcatg atctttgctc gcttggacca gaggggtgaa ctatagagcg
      121 gtttcacact atcgctgaag gtgcattggt agcacgctct aaaattaatt gactatgcgc
      181 gaatggatca acgggcgaaa cccggtttac gaggtgctgc gcgccgcgag gcgacaagtt
      241 ttccgtctgc aaattgccca gggtgtccag gaaaagggtc gcctggctga gatcgcgagc
      301 ctgtgccaac gggggagaat acccattgaa cgccctcaac ggaatcaatt agatgcgcgc
      361 ttccccaaca atcaaggagt ggcacttgag gtgagcagct acccctattc ggatttagtc
      421 gatatgctgg ctctggctga aaagcgcaaa gagccgccat tactgcttat cctggacacc
      481 ttgcaggatc cgcagaacct ggggaccttg ctgcgtacgg cagagattgt tggtgttcat
      541 ggcgtgctgt tgccgctgcg gcatacggcg accgtcactc cagcagtagt acacgcctca
      601 tctggggcta gcgagcatct attgattact caactcaact tggcccaggc aatcgagacc
      661 ttgaaacgag aagatgtgtg ggtggtgggc ctggagggag accctgctga ggcagtgcca
      721 agcgatgtca ggctggatgg ccctctggcg ctggtagtag gcagcgaagg ggaggggatg
      781 cgctcattag tgcgcaagtc ttgcgatgtt ttgttgcgct tgcccatgcg cggccatgtt
      841 gagtcactca acgcagcggt cgccgggtct gtggcgctat acctggcctg gcaggcccgc
      901 aggtggggat agaaatacac catccctata agcagggatg gtgatattca gcagaatggg
      961 gattttgatc cccaaattca gttggcaaga gcattaggct gcattcagcc tgaccctata
     1021 gctgccagca tctcctcatt gctaccgaac ttgaattcat ctagattgcg gcgctcggcc
     1081 tccgactgtt cgacggcttc caggcgcagg atattcgcct tcgtaatgat cggcttgctg
     1141 attttctgga gaagtttatt tacgttgtcc gcaactgccg ttggattctc cattgcaggc
     1201 attgtctgca aatattgcag catggcttga gcgccgcttt ctccgtcttt ccgtgcgact
     1261 cccactaatc ctgcgctggc ttctcgtgac cagccggcga cgaagattcc ttgaatgggt
     1321 tgaccggttt gcgcatcaaa aacttcatgg gatatcccat tcaccgggta gctgggatta
     1381 ggatttttaa caaattcatt ccattgcaca ggcagcccga aagtttcatc caccttgtcg
     1441 ccgatacaga agatgaccgt atccacatcc aggtttcgct tgatcccggt gcccctggct
     1501 ttggtgtcgc cattgaaggg gatgagcttg gtctcttcaa cttccagccc ctgcacctgt
     1561 ccgctggcat ctcccaggat gcgcacgggt gaagccagaa aatcaaagag caggcgggat
     1621 tcagagacgg attcataagc ttttggcagc gctgagagga tgaagtcctt ggcagcctga
     1681 atgtcctgtc caacc
//
LOCUS       DBNHOH010000252         8572 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_398437, whole genome shotgun sequence.
ACCESSION   DBNHOH010000252 DBNHOH010000000
VERSION     DBNHOH010000252.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8572)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8572
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_398437"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            401..2194
                     /gene="metG"
                     /locus_tag="AC16V7_04475"
     CDS             401..2194
                     /gene="metG"
                     /locus_tag="AC16V7_04475"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIR6256407.1"
                     /translation="MTKNILVAVAWPYANSLIHVGNITGSYLPADIFARFQRLRGNRV
                     LMVSGSDAHGTPVSVRADAENSTALQVYQKYHAGFIELFQKLGLAYDLFTSTHTENHF
                     KVSQAIFLALKNNGYLYTERQLQWYAPSQGRYLPDRYVEGTCYICGYASARGDQCDNC
                     GSLLEATQLLNPRSKIDGSTPELRETEHFYLDLHKLQPEIVDFLRQRESYFRPNVLRQ
                     SLGQILANDLHGRAITRDLDWGIPVPVDGWEGKCLYVWFEAVIGYLSATVEWSQVAGQ
                     PEAWHDWWYDPQARAYYFIGKDNIPFHAVIWPGQLIGAGEWFGKLFEGSEDNHLTLPF
                     DVPANEFMNLEGQKISGSRNWAVWGLDFLTRYDPDPLRYYLTANMPESKDTDWAWQDF
                     LNRNNNELVATWGNLANRVLAFAYKHWDGRVPEPSELRQPDREILAQVEAGFQSVGEQ
                     LEAVRLRAGLMEVMRLATEVNKYLDQAAPWFEIKSDKNAAASSIYTALRAIDSLKLLF
                     CPFLPFTSQRLHTYLGYTQPLFGEQYVETYQDELGEHVALRYRPDKGSGRWEPSQLPA
                     GQVLQKPEPLFRKLDESILDEERQRLGKVDA"
     gene            complement(2205..2684)
                     /locus_tag="AC16V7_04480"
     CDS             complement(2205..2684)
                     /locus_tag="AC16V7_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015335287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HIR6256408.1"
                     /translation="MDSKLIELQIGEILTQRKLTMATAESCTGGLVSHRITNVPGSSH
                     YFLGGVVAYANQTKMALLDVRLDTLEKFGAVSEATVLEMARGVRQALNADIGVAVSGI
                     AGPGGGTPEKPVGLVWIGISGWGEDQARSYVWSGDRMAVKEHSAQAALEMLLSMLDK"
     gene            2818..4680
                     /gene="uvrC"
                     /locus_tag="AC16V7_04485"
     CDS             2818..4680
                     /gene="uvrC"
                     /locus_tag="AC16V7_04485"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIR6256409.1"
                     /translation="MDLQSNLQAILDTLPTKPGCYLMKDEAGKVIYVGKAINLRNRVR
                     SYFHDSAQHERRILKLVRKIRDIEWIVVDSELEALILEMNLIKKHRPHYNVRLKDDKR
                     YPYIKVHWGNPFPKITVTRQMVQDGSRYYGPYTSVWAVHQTLDVLRRIFPYLTCDRVI
                     TGNDERACLYYDIKLCTAPCIGAIDQANYQRMIDDFCHFLMGRTEPVVTRLQAEMERA
                     SEALRFEQAAVIRDQLRAIENVVARQRVISSEYVDSDVIAIARADGEACVQVFFIRGG
                     KLIGREYFLLEGTEETPDAGVMAEFIKQFYDQAPSVPQHVLLPHEVEEAQIITQWLNQ
                     KSSGQKVEILVPREGAQHELIEMALQNATETLAALQAQWHADTVRQEQALAELQSALS
                     LTEPPNRIECYDISNTQGTNAVGSMVVFERGVPKKSLYRHFNIRSVAGPDDFASMEEV
                     LTRRFNRWQAAQEASQKPGKRPDTSFSRLPDLILIDGGKGQLGRATAVFDRFGLLGKI
                     PVAGLAKQNEELFVPGRSDPIILSRQSQGLYMIQRVRDEAHRFAITSHRKLRTKQGLA
                     SRLDSIPGIGPARRKLLLNHFGSIQDIQAASMEELTAVSGITSKLAQAIKEHLE"
     gene            4699..4824
                     /locus_tag="AC16V7_04490"
     CDS             4699..4824
                     /locus_tag="AC16V7_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256410.1"
                     /translation="MGQGKKGPTKREIRRVRTQQIIFALIAVIVILSWVISLVSN"
     gene            complement(4921..>8572)
                     /locus_tag="AC16V7_04495"
     CDS             complement(4921..>8572)
                     /locus_tag="AC16V7_04495"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR6256411.1"
                     /translation="NKEGKERWLDFTPNLIEIQFEGRPAGLGTAFDISQRKNAEQSSS
                     QTEVKLRRRAEELSVLHSLSLDISAPLELTEMLQKIVKRSVELLNAAGGAISLCDDEH
                     NKVVVQFENYHALENHHDVVFAYGEGAAGWVAQTGKPLIIDDYRIWDGRASVYEERQP
                     YSAVLSVPMISQGKVIGVLQVMADNMERLFDQSDQELLSLFANQAAIAVENARLLNQA
                     SIEKRHLSLLYDVGRELTTSLDPDEILNRAITLTCQALEGLVGQAFYYVPNNDLLSLR
                     AIYGRPATLLKDFDEQIAINPGRGLAGWTALHRQPVYVPDVTQDERWLFVQGIDDDAR
                     SAISAPILAGERLFGVLSVLHVETGAFSSDQLNLLQGICQEVGLALSNATRYQQAQRQ
                     LAEITLIQNLAQTFNRRLGLQALLNVIVTQLAERLGYPLVEIFLIEGDSLVQRAAYGV
                     AHSQDLIPLTKGIIGRVARTGQAALIPDVYLDPDYIPSFSDTVSELVVPIFHSTVVVG
                     VVNIESRVPGQLTVQDRDLLEVLAVQVSIALENAVLYDHLVQHAADLEHKVVERTIEL
                     TELYELSQKIGYTLSTRDLLQMLLSHLRNALRSDLVMGGMFSNGQRLLIVETNQPLAP
                     DCVTLLHAHWLDMLQSQNISHKEYEDFQPEIVHAKTFDTGHEPLTHIDSLISSPITVA
                     GDVVGLLIAGCARENAFAEAHIRILNTFANQAAVAFQRMAAILAAEQKRLEGLVEHLP
                     VGILLLDSEFRLVVANPLGKEILAELSEEYAEGSLEYLGSQSVAELVARHNDQFPVEI
                     VQRGTARRVFEAQARPAGKETGQWLLVLREVTYEREYQMRIQMQERLATVGQLAAGIA
                     HDFNNIMAAIMVYTDLLSDDPNIPPTSQDRLNTIQQQVQRATSLIRQILDFSRRSVME
                     QCDLDLLPFTKELDKMLRRVLPETIQLELSYRSNKYMVRADPTRLQQALMNLALNARD
                     AMPDGGLLQFELNRILIQPGERPPMEDMLPGEWIRFTISDTGQGISTDKLPHIFEPFF
                     TTKPVGQGTGLGLAQVYGIIKQHDGFIDVKSRKGQGTTFSIYLPASSVPPVEEPVTKT
                     KIQFSGGGRYVLVVEDDQATREALEALLEAHQYSVVTSNNGAEALKLLEDGHTKIDLI
                     VSDVVMPQMGGVALYREVRNRWPQIKFLFITGHPMGGEYQDLLEKGKVLWLQKPFSAQ
                     SFNQSVHSLLEE"
BASE COUNT         2091 a         2085 c         2224 g         2172 t
ORIGIN      
        1 ctctgggctc gtatcttccc ggcttcatag tcaatcacca ttttcagttt gtgctgttct
       61 ttcatcgtta atgtcacttg ttttccatgg ggtgacattt tccctgaaca gtcagggggt
      121 gtcaatatcg ctgaacaaca acaaacttgg gtgcgaacac ttgacaaatc ctgtggccca
      181 atatagactt accaagatct ttctgcaggt tgcttgagat ggtgcgtata ttatcgctca
      241 aggttaatgg gatgaccttc gttatgtcca tgatgatgcg catggaaatg aaatatcgcg
      301 ttcaggcagc cagtgggaat ccggcgtagc cggccctcaa agagatatga caggctgtcc
      361 tgagaaggac agcctttttt attagatgtg ggaggtactg atgacaaaaa acatcctggt
      421 ggcggtggct tggccttatg ccaactcgct catccatgtg ggtaatatca ccggatccta
      481 tcttccggca gatatcttcg cccgcttcca gcgcctgagg ggtaatcgcg tattaatggt
      541 ctctggctct gatgcccacg gtacccccgt ttcagtgcgt gccgatgcgg agaattccac
      601 agctttgcag gtttaccaaa agtaccacgc aggctttata gagctttttc agaaacttgg
      661 tttggcttac gatcttttca ccagcacgca tacagagaat cacttcaaag tgtctcaggc
      721 aatcttccta gcgttaaaaa ataatggata tttgtataca gaaaggcagc ttcaatggta
      781 tgccccctca caggggcgct accttcctga tcgatatgtg gagggcactt gttatatttg
      841 tggctatgct agtgcgcgcg gtgaccaatg cgataattgc ggtagtctat tagaagccac
      901 ccagttatta aatccgcgtt caaaaatcga tggcagcaca ccggagctgc gcgagactga
      961 gcatttctat cttgatttgc acaagctaca gccagagatc gtcgatttcc tccgccaaag
     1021 agagagctac ttccgaccta atgtgttgcg ccaatcatta ggtcaaattc tggcaaatga
     1081 cctgcacggg cgcgctatca cacgtgattt ggattgggga ataccagtgc cagtggatgg
     1141 ctgggaaggc aagtgccttt atgtatggtt tgaagcggtc attggatatc tctccgcaac
     1201 agtagagtgg tcgcaagttg ctggtcagcc tgaagcttgg catgactggt ggtatgaccc
     1261 ccaggcgcgt gcatactact ttattggcaa ggacaacata cctttccatg cagtaatttg
     1321 gccagggcag ttgatcggtg caggtgagtg gtttggtaag ctttttgagg gttcggagga
     1381 taaccatctg acgttaccct tcgatgtccc agcgaacgag ttcatgaact tagaagggca
     1441 gaagatctca gggagccgca attgggcagt gtgggggctg gatttcctca cccgttacga
     1501 tcctgaccca ctgcgctatt acctaaccgc caacatgcca gagagcaagg atacagattg
     1561 ggcctggcaa gattttctca accgcaacaa taacgaactg gtagctacct ggggcaacct
     1621 agcaaaccgt gtgctcgcct ttgcttacaa gcattgggat ggccgagtgc cagaaccgag
     1681 tgaattgcgc caacctgatc gcgagatcct agcccaggtg gaggctggct ttcaatctgt
     1741 gggtgaacaa ttggaggcgg tgcgcctgcg agccggactg atggaggtta tgcgcttagc
     1801 aacagaggtg aacaagtatt tggaccaggc tgccccctgg tttgagatca aatcggacaa
     1861 gaatgcggca gcctcttcaa tctacactgc gttgcgggca attgattccc tcaaattgtt
     1921 gttctgcccc tttcttccat ttaccagcca gcggttacat acctacttgg gctataccca
     1981 gccgcttttc ggggagcagt acgtggaaac ctaccaggat gagcttggtg agcatgtagc
     2041 gctgcgctac cgtcccgaca aaggcagcgg gcgctgggag ccaagccagc tacctgctgg
     2101 tcaggtattg cagaagcccg agccgctgtt tcgcaagctg gatgagagca ttttggatga
     2161 agagcggcaa cggcttggca aagtagacgc ttgatcgcaa taggctattt atccagcata
     2221 cttaagagca tttctaacgc ggcctgggcg gaatgttctt taaccgccat gcggtcaccc
     2281 gaccaaacat agctcctggc ttggtcttcg ccccagccgc taatgccaat ccaaaccagc
     2341 ccaactggtt tttcaggagt gccgccgcct gggccagcga tcccgctaac agcgacgccg
     2401 atatctgcat tgagtgcctg gcgcactccg cgtgccattt ccagcacggt tgcttcactc
     2461 acagcgccaa atttttctaa ggtatccaag cgtacatcca gcaatgccat cttcgtctgg
     2521 tttgcgtatg ccaccacgcc gcccaagaag taatgggatg agcccggcac attggtgatg
     2581 cgatgactga ccaggcctcc tgtgcaggat tcggccgtag ccatagttaa tttgcgttgg
     2641 gtgagtattt caccgatctg caattcgatt agtttactgt ccatggttgt attatatttg
     2701 gattgggcag ttgaataaca gcaatctggg tttaacctat attgtgtcaa actgcgcagt
     2761 ttctaattga tgtgctggga taaatatctg tggggtgact atgttagaat cttggcgatg
     2821 gatttacaat ccaatcttca ggctatcttg gatactttgc ccaccaaacc tggctgctat
     2881 ctgatgaaag atgaagccgg caaggtgatc tatgtcggca aggctattaa cctgcgcaat
     2941 agggtgcgat cctattttca tgatagtgcc cagcatgagc ggcgtatcct aaaactggtt
     3001 cgcaagatac gggatatcga atggatcgtg gtggactccg agctggaggc gctgatcttg
     3061 gagatgaacc tgatcaagaa gcaccgcccg cactacaatg ttcgcctgaa ggacgataaa
     3121 cgttatcctt atatcaaagt tcactggggc aacccttttc ctaagatcac cgtgacccgc
     3181 cagatggtcc aggatggatc gcggtattat gggccatata ccagcgtgtg ggcagtacac
     3241 cagacgctcg atgtgttaag gcgtatattc ccttacctga cctgtgatcg cgtcatcact
     3301 ggaaatgacg agcgcgcttg cctgtactat gacatcaaat tgtgcaccgc accatgtatc
     3361 ggggccattg atcaggcaaa ttaccaacgt atgatcgacg atttttgcca ttttcttatg
     3421 ggtcgcaccg agccggtggt gacgcgtttg caggctgaga tggagcgggc ctctgaagcg
     3481 ttgcgtttcg agcaggcggc tgtgattcgg gaccaattgc gggcgataga aaatgtagta
     3541 gctcgccagc gagtgatctc gtcggagtac gtcgactcgg atgtcattgc cattgcccgt
     3601 gcggatggtg aagcctgtgt gcaagtgttt tttatccgcg ggggtaaatt aattggccgc
     3661 gaatatttcc tgctggaggg tactgaagaa acccctgatg cgggtgtgat ggctgaattc
     3721 atcaagcagt tttacgatca ggcacccagt gtgcctcaac acgtactcct accccatgag
     3781 gtggaagaag cccagatcat cacccaatgg ttaaatcaaa agagtagcgg acaaaaagtg
     3841 gaaatcttgg tgccgcgcga gggggcgcag catgagttaa ttgaaatggc attgcaaaat
     3901 gccacggaaa ccctggcggc gctccaggca cagtggcatg ccgatacagt gcggcaggaa
     3961 caggctttgg ctgaattaca atcggcatta agcctgaccg aaccgcccaa tcggatcgag
     4021 tgttatgata tttccaacac ccagggtacc aacgctgtgg gtagcatggt agtattcgag
     4081 cgaggtgtgc cgaagaagtc gctgtatcgc catttcaata ttcgcagcgt ggccggtcct
     4141 gatgattttg ctagtatgga ggaggtactc acccgtcgtt ttaaccgctg gcaggctgct
     4201 caagaagcct cccagaaacc tggtaaacgt ccggatacat ctttctcaag gctgccagat
     4261 ctgatcttaa tagacggcgg caaaggtcaa ttagggcgcg ccaccgctgt tttcgaccgt
     4321 tttggattgc ttggtaaaat cccagtcgct ggattggcaa agcaaaacga ggaactattt
     4381 gtaccgggaa gaagcgatcc gattatttta tctagacaat cccagggttt atatatgatt
     4441 caacgcgtgc gcgatgaggc acatcgcttt gccatcacat cgcaccgtaa actgcgcacc
     4501 aaacaaggtc tggcgtcccg cttggattct atccccggga ttggtccagc acggcggaaa
     4561 ttgttgctca atcacttcgg ctcgatccag gatatacaag cagcctcaat ggaggaattg
     4621 actgccgtat cagggatcac ttccaagctt gcgcaggcga tcaaagaaca cctggaataa
     4681 aattaaagga gattattaat gggacaggga aagaaaggtc caaccaagcg tgaaatacgg
     4741 cgagtacgca cacaacagat tatcttcgct ttaatcgcgg tgatcgtcat attgtcctgg
     4801 gtaatcagcc tggtatcgaa ttaactataa agactattcc cttctgggtt taaatataaa
     4861 tgaacctgcc agtgtagctt ccatggcatg caggagcgat gatgcgctgg ggttgtaggg
     4921 ttattcttcc agaagtgagt ggacagactg attgaagctt tgggctgaga atggcttttg
     4981 cagccacagc accttgcctt tttctagcaa atcttggtat tctccgccca tggggtgtcc
     5041 agtgataaac aggaatttga tttgaggcca gcgattccgg acttccctgt agagggcgac
     5101 accacccatt tggggcatta ctacatcgct tacgatcaga tcgattttcg tatgcccgtc
     5161 ctccaataac tttagcgctt ccgcgccatt gtttgatgtt actacggagt attggtgagc
     5221 ttcgagcaat gcttctaagg cttcgcgagt agcttggtca tcttcgacta ctaaaacata
     5281 tcttcctccc ccactaaatt ggatcttggt tttcgtgact ggctcttcaa ccggaggcac
     5341 gctactggca gggagataaa tcgaaaatgt ggtgccttgt cccttgcggc ttttgacatc
     5401 aataaagcca tcgtgttgct taatgatgcc atagacctgt gcaagaccca atcctgttcc
     5461 ctgcccgacg ggtttggtgg tgaagaaagg ctcaaatatg tgggggagtt tatctgtaga
     5521 gataccctgc ccggtatcgc tgatggtgaa acgaatccat tcccccggaa gcatgtcttc
     5581 catgggaggc ctctcgcctg gttggattag gatacgattc agctcaaatt gtaataaacc
     5641 tccatcaggc atggcgtctc gggcgtttaa agccaggttc atgagagcct gttgtaaacg
     5701 ggtgggatca gctcgaacca tatacttatt tgaacggtag gataattcca actggatagt
     5761 ctctggtagc acgcgccgca gcatcttatc cagttctttg gtgaaaggta gcaaatccaa
     5821 gtcacactgc tccattaccg agcgacggct gaagtccaag atctggcgga tcaaactggt
     5881 agcacgttgc acttgctgct gaatggtgtt tagtcgatct tggctggtag gaggaatgtt
     5941 cgggtcgtca ctcagcaagt ctgtataaac cattatggct gccataatgt tgttaaaatc
     6001 gtgcgcgatg cctgctgcaa gttgcccaac agtagccagt cgttcttgca tctggatgcg
     6061 catttgatac tcgcgctcgt aggtcacctc acgcaagact aataaccact gacctgtttc
     6121 ttttcccgct gggcgtgcct gggcttcaaa gactcgccga gctgtaccgc gctgcacaat
     6181 ctcgacagga aattggtcat tatggcgggc tacgagttca gcaacacttt gagaccctaa
     6241 gtattccagg gatccctcgg catactcttc actgagctca gctaggatct ccttgccgag
     6301 cggattagcc accaccagcc gaaattcaga atccaataat aggataccca ccggtaagtg
     6361 ttcaaccaaa ccttccaggc gtttctgctc tgcagccaga atagcagcca tgcgttgaaa
     6421 agcgactgca gcctggttag caaaagtatt caaaatgcgt atgtgcgctt cggcaaaggc
     6481 attttctcgt gcacacccgg caatcaacag accgactacg tctccggcga ctgtgatggg
     6541 tgagctgatc agtgagtcga tatgcgtcaa aggctcatga ccggtgtcga acgtcttcgc
     6601 atggacgatc tctggctgaa agtcttcata ttctttgtgc gatatatttt ggctttggag
     6661 catgtccagc cagtgagcgt gcaaaagagt tacgcaatcg ggtgccagag gctggtttgt
     6721 ctcgacgatg agcaatcgct gtccattact aaacatgcct cccatcacta ggtcgctgcg
     6781 tagcgcattg cgcaggtggc taagcagcat ttggagcaaa tccctagttg atagcgtata
     6841 gccgattttt tgacttaatt catacagctc ggtaagctca atcgtgcgct ccaccacttt
     6901 gtgttccagg tcggcggcat gttgcacaag gtgatcatat aaaaccgcgt tttctaatgc
     6961 tatcgaaacc tggactgcca gcacctcaag cagatcgcga tcttggacgg tcaattgtcc
     7021 aggaactcga gattcaatgt ttactacccc aaccaccact gttgagtgaa atatcggaac
     7081 aaccagttca gagactgtgt ccgagaagct agggatatag tctggatcga ggtatacatc
     7141 gggaatgaga gcggcttgtc ctgtgcgcgc tacacgccca atgatgcctt tggtaagcgg
     7201 gataagatct tgggaatggg cgactccata ggcagcacgt tggaccagag agtcaccttc
     7261 gatgaggaaa atctctacta gcggatatcc caagcgttcg gctaattgcg tgacaatgac
     7321 gttgagtaat gcctgcagac ctagtcgtct attaaatgtt tgcgcgaggt tttggatgag
     7381 ggtgatctca gctaattggc gctgggcttg ctggtaacgc gttgcgttgc tgagagctaa
     7441 tcccacctcc tgacagatac cttgtaacaa attcagttga tcagaggaaa atgcgccggt
     7501 ctcgacatgc agcaccgaga gcacaccaaa tagtcgctcg ccagcgagaa tgggagcgct
     7561 gatcgccgag cgggcatcat catcaatacc ttgaacaaat agccaacgct catcttgggt
     7621 gacatcgggc acgtaaactg gttggcgatg caaagcagtc caacccgcta atcctcggcc
     7681 aggattaatg gcaatttgct cgtcgaaatc tttaagcaag gttgctggac ggccataaat
     7741 ggcacgcagg ctcaaaaggt cattattggg aacatagtag aacgcctgac caactaatcc
     7801 ttccagagcc tggcatgtaa gagtaattgc tcggtttagg atttcatctg gatcaaggct
     7861 ggtggttaac tctcgcccta catcgtaaag caggctgagg tgacgcttct cgatagatgc
     7921 ctggtttaag agacgcgcat tctcgaccgc gatggcggcc tgattggcaa ataatgacag
     7981 taattcctga tcggattgat caaagagacg ctccatattg tcagccatca cctgtaagac
     8041 accaataacc tttccttggg aaatcatggg tacactgagg acagcggaat agggttgcct
     8101 ttcctcgtaa actgatgcgc gaccatccca gatgcggtaa tcgtcgataa tcaatggttt
     8161 gcctgtttgg gcaacccacc cagccgctcc ctctccataa gcgaaaacca catcatgatg
     8221 attttctaga gcgtgataat tttcgaactg tacgactacc ttattatgct catcgtcaca
     8281 cagagagatt gcgccaccag ctgcatttaa aagctctaca gagcgcttga caatcttttg
     8341 cagcatttcg gtgagttcga gcggtgcaga gatgtccagc gagagagagt gaagaactga
     8401 aagttcttcg gcgcgacggc gtaatttaac ctcagtttgg ctgctggatt gctcagcgtt
     8461 ctttctctgg gaaatatcga aagcagtccc cagaccagct ggtctgcctt caaactggat
     8521 ttcaatcagg ttaggagtga agtctaacca gcgttctttc ccctccttat tg
//
LOCUS       DBNHOH010000253         2289 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_976060, whole genome shotgun sequence.
ACCESSION   DBNHOH010000253 DBNHOH010000000
VERSION     DBNHOH010000253.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_976060"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="AC16V7_04500"
     CDS             complement(<1..572)
                     /locus_tag="AC16V7_04500"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR6256412.1"
                     /translation="MWNLTLRSPEGKPREFQISKSKTTIGRRSGNDIIVADISASRIH
                     AVIEHHDQQDSLIIRDLGSTNGTFVNRERLVKPRYLRHQDVIRIGEHVINVVKNQVGS
                     NVRAGSVTRQLTRDVVLESIDQHAVLIYEISRKLNNVLNIDSALKEVSNMMRQALGAD
                     RCVVMLVDQFEKLSQMGFPNSIADLVIKQRS"
     gene            828..2114
                     /locus_tag="AC16V7_04505"
     CDS             828..2114
                     /locus_tag="AC16V7_04505"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6256413.1"
                     /translation="MPDNKNLKQLLAEPHKMEDLHIPQNIVIDIILRLLFNEGNVALG
                     RFVDVLKVSNRIIDDVMLWMQKEHLVEVSKASSELGRLGYVYTLTDAGKQRARDALDR
                     SLYVGPVPVPIHYYNRGMELQTKQRRRIPADQVKEALKPLVLPPDFHRRIGPAINSAS
                     SLFLYGPPGNGKTTIAQGIGNLIAGVEPIWIPYALTAGGHIIQIHDRLIHKSITIEGG
                     RTEELGRVDQRWGLFTRPSVIVGGELKMEALDLRFDPVAKIYEAPLQLKANGGMFLLD
                     DFGRQQVRPSDLLNRWIVPLENEVDFLRLLSGQTIVVPFRMLIVFSTNLDPNELVDDA
                     FLRRIQIKVEVVAPDEKLFYQIFMNMCKYFNIPFERESFVYLLQNWYRQNGRVMQAVH
                     PRDIIRTIIALCDYEGIQPKLTPELIDDACRTYFVK"
BASE COUNT          657 a          536 c          488 g          608 t
ORIGIN      
        1 gatctttgct taatcaccaa atcggcgatt gaattgggaa agcccatttg cgaaagcttt
       61 tcaaattgat ctaccagcat cacaacgcac cgatcagccc ccaaagcctg tctcatcatg
      121 ttagatactt ctttgagagc ggaatcaata ttcaacacat tgttgagctt tctagaaatt
      181 tcatagatca agacagcatg ttggtcaatc gattcgagaa ctacatctcg cgtgagttgt
      241 cgtgtaactg atccagcacg aacattgctg ccaacttgat tttttactac attgataaca
      301 tgttcgccta tacgaatgac atcctgatgg cgtagatacc gcggctttac tagccgctca
      361 cgattgacaa atgtgccatt ggtactacca agatctcgaa tgatcagaga gtcttgttgg
      421 tcatgatgct cgatcacagc gtgaattcgc gacgcagaaa tgtccgctac aattatgtca
      481 ttacctgatc tgcgcccaat agtcgttttt gatttagaaa tttgaaattc acgcggtttt
      541 ccttctggag aacgcaaagt taaattccac attgtatgct aaagccctct ttcctgcgcg
      601 tcaatcacct agaaatcaat agcacatcat ttcaatttta ttaccaaaca agatatattt
      661 ttccttgaac atgagaactt tctatattgt tagcgcagca attccatggc ttgctaccat
      721 cggctggatg gtaaaattca agggggatag gtgccaatcc ttgcacctct ttatccaggt
      781 tatcataaaa ctacagaagg catactcccg aacacggaag gtaacgaatg ccagataata
      841 agaacttgaa gcaacttcta gccgaacctc ataaaatgga ggacctgcat atcccccaaa
      901 acatcgtgat agacattatt ttacgtttac tatttaacga aggcaatgta gcactaggta
      961 gatttgttga cgtacttaag gtgtccaacc ggattatcga tgatgtaatg ctgtggatgc
     1021 aaaaagagca tctggtcgaa gtatccaagg cgagttccga attaggtcgt ctaggatatg
     1081 tttatacact caccgacgca ggaaaacagc gtgcccgcga cgctctggat cgcagtcttt
     1141 acgtaggccc agtacctgtc ccaattcatt attacaaccg tgggatggag ctccaaacca
     1201 agcaacgcag gcgcataccc gcagatcagg tgaaagaagc cctgaaacca ctcgtcttac
     1261 cccccgattt tcaccgccgg attggccctg ccataaactc tgcctcatca ttgttcctgt
     1321 atggtccccc aggaaacggc aagacaacta tagcccaggg tattggcaat ttgatcgccg
     1381 gcgtggagcc aatctggata ccttatgctc tgaccgctgg tgggcacatc attcaaattc
     1441 acgaccgcct gatccataaa tcgattacca ttgaaggcgg acggactgaa gaattagggc
     1501 gtgtagatca gcggtggggt ttatttaccc gcccatcagt aatagtcggt ggtgagctaa
     1561 aaatggaagc gctggatcta cgctttgacc cggtagcaaa aatatacgaa gcaccgctac
     1621 agctcaaagc gaatggaggc atgtttctcc ttgatgattt tgggcgtcag caagtacgac
     1681 catcagacct acttaatcgc tggatcgtac cgcttgaaaa cgaggtcgac tttctccgct
     1741 tgcttagcgg gcagacaatc gtagtcccct tccgtatgct catcgtattc agcaccaatc
     1801 tagacccaaa tgaattggtg gatgatgcct tcttgcgacg aattcaaatc aaagtagagg
     1861 ttgtcgcgcc agatgaaaag cttttctatc agatattcat gaatatgtgt aagtatttta
     1921 atattccttt tgaacgtgaa tctttcgtat atcttctcca aaattggtac aggcaaaatg
     1981 gtcgagtcat gcaagctgta cacccaaggg atatcatccg aaccatcatc gcactttgcg
     2041 attacgaagg catacaacca aaattaactc cagaattaat tgacgatgcc tgcagaacat
     2101 attttgtaaa ataagtcggc gccaggaggt aagagatgcc tagtatcgta caggaaaaaa
     2161 ttaaccaggc gatcaatatt ttacgcgata aaaatgtaga tgtatggctc acttttgtcc
     2221 gcgagacgac cgcatccggt gaccctgtat taccgctcat ctatggaggt aacctgacct
     2281 ggcaaagtg
//
LOCUS       DBNHOH010000254         2289 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_719827, whole genome shotgun sequence.
ACCESSION   DBNHOH010000254 DBNHOH010000000
VERSION     DBNHOH010000254.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_719827"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..1802
                     /gene="fdrA"
                     /locus_tag="AC16V7_04510"
     CDS             66..1802
                     /gene="fdrA"
                     /locus_tag="AC16V7_04510"
                     /inference="COORDINATES: protein motif:HMM:NF004760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HIR6256414.1"
                     /translation="MTIIEAEIRSGVYYDSVILMQLQRSLIELSGILDAGVVMGTPAN
                     KDILAQSNLLFPEVEAAVADDLVIVVKGSDRESAKAAINKVDELLVTRRGTETEHEYR
                     PKSLDTAIQLLPEAQWVMISVPGRYAARIARQSLNSGKNVFVFSDNVLLDDEIEFKKL
                     AAAKGLLVMGPDCGTAIINGVGLGFANKVRRGSIGIVAASGTGLQQVSARIHQFGGGI
                     THAIGTGGRDLSSDVNAVTARQGLELLNRDANTQVIVLVSKPPSASVADELVEMARQF
                     RKPVVINFIGYSTQIRVVDNVHFATTFDETAQLAVQLANSASISEPARDLGVERFAPG
                     QRYLRGLFSGGTLAYETLLILENYLPEVFSNAPLDKKFRLSDSLVSQAHSVVDLGEDE
                     FTVGRLHPMMDNDLRIRRLETEANDPQVAVILLDVVLGFGAHADPAAELAPAIAKAKA
                     KVKEAGRYLEVVVVVSGTDEDPQDLDAQVQMLIDAGGVVETSNDKAVRYVGQLLRSLN
                     PELRQAGKEPLPQVDLAAINRPLAAINIGIESFTESLESQNAQVIQVDWRPPASGNEK
                     LMSILERMKSKS"
     gene            1811..>2289
                     /locus_tag="AC16V7_04515"
     CDS             1811..>2289
                     /locus_tag="AC16V7_04515"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR6256415.1"
                     /translation="MLKEFIAACVQIAIKPNDVQANIDKAVVWLKKAVTDYKAELVVF
                     PETVTTGYETDLTPEELWDLVDEAPGSITSAIQMTAKELGTHVVWPSYRRGGERGVVY
                     NSAILIGPNGEIVGLYDKTHPAPWERRDGGGWVELGTRADVFETELGTIGMIICYDG"
BASE COUNT          583 a          530 c          652 g          524 t
ORIGIN      
        1 ataaaacggt ctaaaaatca ctatccatgc ttatcaaaac acagggttac aatttggatt
       61 gaacaatgac aataattgaa gcagaaattc ggtctggtgt atattacgat tcagtcatct
      121 tgatgcagct gcagcgttcc ctgatagagc tttcaggaat actcgatgcc ggggtggtaa
      181 tggggacacc cgcgaacaaa gatatacttg ctcaaagcaa cctgcttttt ccagaagtcg
      241 aggctgccgt cgcggatgat ctggtcatcg tcgtaaaagg cagtgatcgg gaatccgcca
      301 aagcagcaat taataaagtg gacgagttgc tcgtgactcg ccgtggaact gaaacagagc
      361 atgaatatcg tccaaagagc ctggatacag caattcaatt actacctgaa gcgcagtggg
      421 tcatgatctc agttcccggg cgctatgcgg cacgtatagc ccgccagtct ctcaattcag
      481 gcaaaaacgt gtttgttttt agcgataacg ttttactgga tgatgagatc gagttcaaga
      541 agctagccgc tgccaaaggg ttgctggtaa tggggccaga ctgtggaaca gcgattatca
      601 atggggtcgg tctgggattt gccaacaagg tgcgccgtgg atcgattgga attgtagccg
      661 cgtcgggcac cgggttgcag caagtgagcg cgcgcattca ccaattcgga ggcgggatca
      721 cccacgcgat tgggacaggc ggtcgagatc tatccagtga tgtcaatgca gtaactgcac
      781 gccaggggct agagctacta aacagggatg cgaacacaca agttatcgtg ttggtttcca
      841 agcccccgtc tgcttcagtg gcggatgaat tagtggagat ggctcgtcaa ttcagaaagc
      901 cagtagtcat caacttcatt gggtattcaa cgcagattcg cgtcgtggat aatgtccact
      961 ttgccaccac ctttgacgaa actgcgcagc tggcagttca actggcaaac tcagcatcaa
     1021 tatcagaacc tgcaagggat ttaggtgtcg agcgtttcgc gcctggacag cgctacctga
     1081 gaggtctctt ttctggaggc accctggcat atgagacctt gcttattttg gagaattatc
     1141 tgccagaagt attctccaac gccccgctgg ataaaaaatt tcgcctgagt gactcgctgg
     1201 ttagccaggc acattccgtt gtggatctgg gagaagacga atttacagtg ggtagactgc
     1261 acccaatgat ggataatgat ctgagaatcc gccgtttgga gacagaagct aacgatccac
     1321 aggtggcggt tatcttgctg gatgtagtgc taggtttcgg cgctcacgct gatcctgcag
     1381 ccgagctggc gcctgccatt gcgaaggcaa aggccaaagt taaggaggcg ggacgctatc
     1441 tggaggttgt cgtggttgtc tctggcacgg acgaggaccc acaagactta gacgctcagg
     1501 tgcagatgct tattgatgcg gggggagtag ttgagaccag caacgataaa gctgtccgtt
     1561 atgtcggtca gctcttgcgg agtctcaatc cagagctccg tcaggccggg aaagaacccc
     1621 tgcctcaggt ggatttagcg gcaataaacc gccctctcgc agcgatcaat atcggcatag
     1681 agtcattcac ggaaagcctg gagagccaga acgctcaggt tatccaagta gattggcgcc
     1741 cccctgccag tgggaacgaa aaattaatgt ctatactgga acggatgaag agcaagtctt
     1801 aggaaggtgc atgttgaaag aatttatcgc tgcctgtgtc caaatagcta tcaaaccgaa
     1861 tgacgtacag gccaatatcg acaaggctgt cgtctggctt aaaaaggcag tcactgatta
     1921 caaggctgag ttggttgtat ttccggagac ggtaaccaca gggtatgaga ctgatctcac
     1981 accagaggaa ttatgggatc tggttgatga ggctccgggt agcattacca gcgctatcca
     2041 gatgaccgcc aaagagttag ggacccacgt ggtgtggccc tcataccgcc gggggggtga
     2101 gcgaggtgtg gtctataact cagccattct tatcggacca aatggggaga tcgtgggtct
     2161 ttatgacaag actcaccctg ccccatggga gcgccgcgat ggcggtggtt gggtagagct
     2221 gggcacccgg gcggacgtgt tcgaaaccga gctaggcacc atcggtatga tcatctgcta
     2281 cgacggtga
//
LOCUS       DBNHOH010000255         1579 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1745269, whole genome shotgun sequence.
ACCESSION   DBNHOH010000255 DBNHOH010000000
VERSION     DBNHOH010000255.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1745269"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..524)
                     /locus_tag="AC16V7_04520"
     CDS             complement(189..524)
                     /locus_tag="AC16V7_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018888485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HIR6256416.1"
                     /translation="MAYDKNLAARMRQILDELNPPSLTEKHMFGGVGYLVQGNMACGV
                     HKDELIIRVGPEAYIDSLNLPGARPFDLTGRPMNGWVSVRSAGFETDHSLRDWIEQGL
                     AFAKTLPAK"
     gene            complement(589..1059)
                     /locus_tag="AC16V7_04525"
     CDS             complement(589..1059)
                     /locus_tag="AC16V7_04525"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6256417.1"
                     /translation="MKTDIIIDYLADHPDLLAVLASWFYAEWGHSKPDFTIHFFEDEL
                     RGRMNRDQLPLTLVAFLNDNPVATVSLLLEEMETHPQFLHWLGSVYTLPDYRHHGIGS
                     QLLEHAASEAKRLGVRELYLWTRHVESLYARFGWVIIQRPVYRGRPVAIMRRSL"
     gene            complement(1116..>1579)
                     /locus_tag="AC16V7_04530"
     CDS             complement(1116..>1579)
                     /locus_tag="AC16V7_04530"
                     /inference="COORDINATES: protein motif:HMM:NF027484.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HIR6256418.1"
                     /translation="EAVYLPPTRAIGAVPPIIQATATPIPEQAPTQAVTSTINPPCTD
                     NLLYLEDQTIPDGSLVSAGEVLDKRWFVENNGSCNWGKDYRLKLISGFDLGALPEQGL
                     YPARSGTQAAIRIVFTAPAEPGVYRSAWQASNPAGQPFGDPIYIEVQVASD"
BASE COUNT          365 a          410 c          425 g          379 t
ORIGIN      
        1 aaaatggccc gagttagcca caatggctcc atccttcatc acttcgacgt gctgactgcg
       61 caacaggtga agtacttaaa ttcttgggaa gaaggaacgt aactatcaag tgcctgaaat
      121 cgcaggcagc gcgatatcga acgcaggtct ttcccttgta tccccgcccg gtaatgggcg
      181 gggatttttt atttggctgg cagggtcttg gcaaaagcca ggccctgctc aatccaatct
      241 cgcagcgagt ggtcggtttc gaaacccgct gagcgaacgc taacccagcc gttcatcggc
      301 ctgccagtca ggtcgaaggg gcgggcgccg gggagattaa gggagtctat ataggcttct
      361 ggcccgacgc ggatgatcaa ttcatccttg tgcacgccac aagccatgtt cccttgcacc
      421 aggtagccta ctccaccgaa catgtgcttc tcggtcaggc ttgggggatt taattcgtca
      481 agtatctggc gcatgcgtgc cgccagattt ttatcgtaag ccactcagac cttctccatt
      541 ctccttatat cgtgtaactt ctgccagaaa ctaacaggct tttggttttt ataaacttct
      601 tcgcatgatg gcgaccgggc gtccccgata gacaggtcgt tggatgatga cccagccaaa
      661 gcgagcgtag aggctctcaa catggcgggt ccagagatat agctcgcgta cgcccaggcg
      721 cttcgcctcg cttgctgcat gctcgagcaa ttgcgagcca atgccatgat ggcggtaatc
      781 tggtaaggtg taaacgcttc ccagccaatg taaaaattgc gggtgagtct ccatttcctc
      841 taacaataag ctgacggtgg caaccggatt atcgttcaga aaagctacca gagtcagtgg
      901 tagctgatct cgattcatac gacctctcaa ttcatcctcg aaaaaatgga tggtgaagtc
      961 gggcttacta tgaccccatt cagcatagaa ccaggatgca agaacagcca gcaggtctgg
     1021 atggtctgcc agataatcaa taatgatatc ggttttcatg atgggagcct gctctctatg
     1081 actgataatt cacttctcag cctttcccca ttccctcaat ccgaagcaac ctgtacttcg
     1141 atatatattg ggtcgccaaa cggctggcca gcggggtttg acgcttgcca agcgctgcgg
     1201 tatacccctg gttctgcagg ggcagtaaac acgatgcgaa tagcagcctg cgtgccgcta
     1261 cgtgctggat agagtccctg ctcgggtagc gcacccaggt caaaaccgga aatcagcttg
     1321 aggcggtagt cttttcccca gttgcaggaa ccattgtttt ctacgaacca gcgtttgtca
     1381 agcacctcgc ccgcagagac cagagagccg tctgggatag tctgatcttc aagataaagc
     1441 aggttgtcag tgcagggtgg gtttatggta gaagtgactg cctgggtggg agcttgctct
     1501 ggaattggag ttgccgtcgc ttggatgatc ggcgggacag ccccaattgc gcgagtagga
     1561 ggcaggtaga cagcttcct
//
LOCUS       DBNHOH010000256         3467 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1552636, whole genome shotgun sequence.
ACCESSION   DBNHOH010000256 DBNHOH010000000
VERSION     DBNHOH010000256.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1552636"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3295)
                     /locus_tag="AC16V7_04535"
     CDS             complement(<1..3295)
                     /locus_tag="AC16V7_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HIR6256419.1"
                     /translation="MSISGMDVRIFQGTDPTGELLELQSLETSSGPVAYQFVNFTSRV
                     TLDPQLGGATQYVVAVVQPPGLPRMPKNQQGCSFEGTATSCLDLREKDYGWANGKGEK
                     NVQPWALPDIHIEGVTGSVLVSSAGALSVFSADHPASLAGFSQTFSFDTWEVTAEVEE
                     AACIPGGEVTTVVRGHGFVGLPTVGTDGSGPPPWIEVDFKLCGTDLQQAHLELSISPP
                     GIPVANTGVGVILIGGTVTIGPNSTEIKLDIGFQTMDNYLFTDGVGVVTINTAGMFSL
                     KASGKLVGKALIANQVLLEVAWNPLDVLVDAQVSYQNLLWGSLRMHTWVGSGWQNKYP
                     WIQDNKTHFTGQIGAKVQIPTGELINKKYLKVPPFTISISAEVAFGEFCNKAGCAPPY
                     PWGMSATVKVFGYGVGVYVDKSGPEIFIGSSSHKLIDQFGGSAQSAQLLDASLGEVAE
                     ADPVVYNPGAQQAYLAPPFKTPTDTWLAESPATSCTGSAPTITCKFYVDAGIGKALFV
                     ASWQNGGLGVTLIKPDNTEISPGNALAHGITISNTVETNLTMVAFAAQPYGGATSLTQ
                     GDWKVRLNNVGVGLPAGFFNNYQLMFAADPPAPVVSWTTPAAPGVTPDGSGNVNLSWS
                     VMRGGSPLPPFEVPMKLFYIPAAQKPITPTVITGTLIADQIYASVGSHQWNTKGLASG
                     EYAVGARLDDHLKSNGHVVSWAPGTVVVNDITPPPIPSVVGNIAVQGGVITTWYKDLV
                     TPDLAGYLVEYSIPDWDLTSHLPRVRRVIPRPRNEANYLDIFERVRLGGLPITVSIPI
                     NTIICVRAYDASGNVSDCVPDEYTMPEALDERLGPVSALSGDTTGDGAIDVGWDVPTF
                     GTADGYLLGYKPTRCLLPDVVYVADEGPPPITLFPPPFDTTLHGLTPGQWYKFWMQPY
                     NNLGDIGPAVSFEKMILDYTDGDFDGLPDQWEAIFGVSDPSADEDEDLLINLHEYELG
                     SDPLNADSDGDGFYDSEEANPEFGLAISDPCGPDTPPAHEGPKLAVVGLAEMNFTSAV
                     NLPPPPQYVQVMNLGGGALNWTVAASHSWIVLDEPAGDGYGAIPIRVDPKAVRSYGTY
                     VGQITITGGP"
BASE COUNT          695 a         1082 c          969 g          721 t
ORIGIN      
        1 ctggcccgcc agtgatcgtg atctgcccaa catacgtacc gtatgatcgc accgccttcg
       61 gatcaacccg gatgggaatc gccccatagc catcgccagc aggttcgtcc agcacgatcc
      121 aggaatggga ggcagctacc gtccagttca gcgccccgcc acccaggttc atcacctgaa
      181 catactgagg cggagggggc aggttgacgg ccgaggtgaa attcatctct gccagcccca
      241 ccaccgccag cttaggtccc tcatgagctg ggggagtatc cggcccgcat ggatcgctta
      301 tcgctaagcc aaattcagga ttagcctctt cgctgtcgta gaagccatcc ccgtcgctat
      361 cggcgttcag cggatcggag ccaagctcgt actcatgcag gttgatcagc aaatcctcgt
      421 cttcgtctgc gctcgggtcg ctcaccccga aaatcgcctc ccactggtcg gggagtccgt
      481 cgaaatctcc atccgtatag tcgaggatca tcttttcgaa actgaccgcg ggaccgatat
      541 cccccagatt attgtacggc tgcatccaga acttgtacca ttgccctggc gtcagcccgt
      601 gcagggtggt gtcgaatgga ggagggaata acgtgatcgg tggcggccct tcgtctgcca
      661 cgtagaccac atccggcagc aggcaacggg tgggtttgta gcccagcagg taaccgtcgg
      721 ctgtgccgaa tgtgggcaca tcccagccca catcgatggc gccatcaccg gtggtatctc
      781 ccgacaacgc cgagaccggt cccagacgct cgtccagcgc ttccggcatt gtgtactcat
      841 ccggcacaca atcgctcacg ttgccgctgg cgtcatacgc ccgcacacaa atgatggtgt
      901 tgatcggtat agagacggtg atgggtaacc cgcccagccg gactcgctcg aagatatcca
      961 ggtagttcgc ctcgttgcgc ggccttggga tgacgcgccg cacgcgcggc aggtgagaag
     1021 tcaaatccca gtctgggatg ctgtactcca ccaggtagcc tgccagatcg ggcgtaacca
     1081 gatccttata ccaggttgta atcaccccgc cttgcacggc gatatttcca accaccgatg
     1141 gaataggcgg aggggtgata tcgttgacca ccaccgtccc aggcgcccac gagactacat
     1201 gcccgttgga tttgaggtgg tcgtctagcc gggcgccaac tgcgtattcg ccagaggcca
     1261 gtcccttggt attccattga tgactcccca cgctggcgta gatctggtct gcaatcagcg
     1321 tgccggtgat caccgtcgga gtgatgggct tctgcgctgc agggatgtag aacagcttca
     1381 tgggcacctc gaagggcgga agcgggctcc cacctcgcat gacgctccac gacaggttga
     1441 cgttcccgct cccatccggc gtcacaccag gagcggcagg ggtggtccag ctcaccaccg
     1501 gcgcaggcgg gtcggcagca aacatgagct gataattgtt gaagaagcct gctggcaacc
     1561 ccacacccac attattcagc ctgaccttcc agtctccttg cgtcagggag gtcgcgcccc
     1621 cataaggctg cgcggcgaag gctaccatcg tcaggtttgt ttccaccgtg ttcgagatgg
     1681 tgatcccatg tgccagggcg ttgccaggtg agatctcggt gttatccggc ttgatcaggg
     1741 tgacgcccag cccgccattt tgccagcttg ccacgaagag tgccttgcca atccccgcgt
     1801 cgacgtagaa tttgcaggtg atggttggcg cagatccagt gcaggaggta gccggggatt
     1861 ccgccaacca ggtgtcggtc ggcgttttga agggtggcgc caggtaggcc tgctgggcgc
     1921 ctggattgta gacgacggga tccgcctcag ccacttctcc cagggaggcg tctagcagtt
     1981 gtgccgactg ggctgacccg ccgaattgat cgatcagctt gtggctgctg ctgccgatga
     2041 agatctccgg gccgctctta tccacataca cacccacgcc atagccaaac actttcactg
     2101 tcgcggacat gccccacggg tacggcggcg cacagccagc cttgttgcaa aactctccaa
     2161 aagccacttc agccgagatg cttattgtaa acgggggcac ctttaaatat ttcttgttga
     2221 tcaactcacc tgtgggtatc tggactttgg ccccgatttg cccggtaaag tgggtcttgt
     2281 tatcctgaat ccacgggtac ttattctgcc agcctgaccc cacccaggtg tgcatgcgca
     2341 ggctacccca taacaggttc tggtatgaca cctgggcatc caccagtaca tccaacgggt
     2401 tccacgccac ctccagcagc acctggttgg caatcagcgc ctttccgacc agtttaccgg
     2461 atgctttgag cgagaacata ccggcagtgt tgatcgtcac cacccccacg ccatcggtaa
     2521 acaggtaatt atccattgtc tggaaaccga tgtccagctt gatctcggtt gaattcggcc
     2581 caatggtgac cgtcccgccg atcagaatga cgcccacgcc agtattggca accggaatgc
     2641 ccggcggcga tatggacagt tccaggtggg cttgttgcag gtccgtcccg cacagtttga
     2701 agtccacctc gatccacggt ggcgggccac taccgtcggt ccccaccgtc ggtaacccga
     2761 caaaaccatg acctcttaca accgtggtca cctccccgcc gggtatgcag gcggcttcct
     2821 cgacttcagc cgtcacctcc catgtgtcga agctgaacgt ctgggagaag cccgccaggg
     2881 aagctgggtg gtctgcactg aatactgaaa gcgctcccgc gctgctcacc agcaccgagc
     2941 ccgtcacccc ttcgatgtgt atgtccggca gtgcccaggg ctgcacgttc ttctccccct
     3001 tgccgttagc ccagccgtaa tctttctcgc gtaagtccag acaactggtc gcagttccct
     3061 caaaggaaca tccctgctgg ttcttcggca tgcgcggcag gccgggcggc tgcacaactg
     3121 ccaccacata ttgggtagcc ccacctagct gcgggtcaag cgtcacccgg ctggtgaaat
     3181 tgacaaactg gtaagccacc gggccagagg aagtttccaa agattgcagt tccagcagct
     3241 caccggtagg gtcggttccc tggaagatgc gcacatccat ccccgagatg ctcaatggct
     3301 gaggctccaa tggctgccaa tgagttatcg gcaatggatc aaaggtgtat ttgccacatg
     3361 cagtgcatcc tggctggttg ggatataacc gccagttaaa ttcccacaag ctctcctgcg
     3421 ggggctgggc gatatctacg gtaacctggg tggtcagcac cacttca
//
LOCUS       DBNHOH010000257         1834 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_591045, whole genome shotgun sequence.
ACCESSION   DBNHOH010000257 DBNHOH010000000
VERSION     DBNHOH010000257.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_591045"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="AC16V7_04540"
     CDS             complement(<1..455)
                     /locus_tag="AC16V7_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256420.1"
                     /translation="MELMFLGLTSAQWTEAGISLLIVIAVVILGRRLVAIVLDRGIKR
                     LTSSTKTNFDDAILNTLRLPSYWLIVVLSLDYALRRLDFITERWGEQTDSLLYILYLL
                     VGFAFAWSLIQNMFAWYGEEIATRTETDLDQQLMPFFRRVALIILTAVGL"
     gene            complement(546..1205)
                     /locus_tag="AC16V7_04545"
     CDS             complement(546..1205)
                     /locus_tag="AC16V7_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256421.1"
                     /translation="MSLIGVVYTGLPFLAPLLMHIGWTSAGSAIYTIYSLLCHQLPQR
                     SFFLFGNRFTYSLIEIQSVWENSINPLVLRKFIGNSEMGWKVAWSDRMVWMYVSILFF
                     GLLWWLLRYRSKPLPWQGLILFLLPIVLDGTTHFFSDLAGIGQGFRNDNVWLRILTRE
                     AFSISFYTGDAWGSFNSLARLVTGILFGMGIAWFGYPYLNEFFVQISNSVTFEGGAKG
                     G"
     gene            complement(1306..1620)
                     /locus_tag="AC16V7_04550"
     CDS             complement(1306..1620)
                     /locus_tag="AC16V7_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256422.1"
                     /translation="MILDKPCDHLVDWAVQKLTNAGLQVMPTFNLQAARLSHPDCPCP
                     YHGTQACDCQMVVLLVYTDEPQPASLVIHMHHGRALFYLVDTPQQRAGEHLIALIQGA
                     LL"
BASE COUNT          531 a          477 c          436 g          390 t
ORIGIN      
        1 agccctaccg cggtcaagat gatcaatgca acccgccgga agaagggcat taattgttga
       61 tctaggtctg tttcggtgcg tgtggcaatc tcctctccgt accaggcaaa catattctgg
      121 attaagctcc aggcaaaggc aaatcccacc aacagataaa gaatgtagag gagcgagtct
      181 gtctgttcgc cccaccgctc ggtaataaag tccagcctac gcagggcata atcaagggat
      241 aagaccacta tcagccagta agacggcagg cgcagggtgt tgagaatggc gtcatcgaag
      301 ttcgtctttg tactggaggt taacctcttg atacctcggt caagcacaat cgccaccaac
      361 ctgcggccca agatcacgac cgcaatgacg atgagcaaag atatcccggc ttcggtccat
      421 tgggcactgg ttaatcctag aaacattaat tccataccaa actcctgcag agattatatc
      481 aaaaggaaaa tcttacaggt cttctcgctt taacaaaaac ccaatgaaga atattataaa
      541 gccagctacc ccccttttgc gcctccttcg aaagttaccg agtttgaaat ctgcacgaaa
      601 aattcgttca agtacggata gccgaaccaa gctattccca tcccaaacag aatcccggtt
      661 actaggcgcg ccagtgagtt gaacgatccc caggcatctc ctgtatagaa gctaatcgag
      721 aacgcctcgc gggttaggat tctaagccac acattgtcgt tgcgaaaccc ttggccgatc
      781 ccagctaaat cgctgaaaaa gtgcgttgtc ccatctaaca caatcggaag aaggaaaaga
      841 ataagtcctt gccaaggcaa gggttttgat cgataccgaa gcaaccacca gagcaaacca
      901 aaaaatagga tgctgacgta catccatacc atgcgatctg accaggcaac tttccagccc
      961 atctccgaat taccgataaa cttgcgcaac accaatggat tgatgctatt ctcccaaaca
     1021 gattgaattt caatcaggga ataggtaaat cggttgccaa acaagaaaaa cgaacgttgt
     1081 ggcaattggt ggcaaaggag tgaatatata gtgtagattg cactacccgc gcttgtccac
     1141 ccgatgtgca tcagaagcgg cgccaggaaa ggcaaccctg tataaaccac cccgattaga
     1201 ctcacccaaa atatccagcg cctgcaaaaa gcatttacca ggcgcccaag aattgatcgg
     1261 tagcgtatag attgcgcgct caatgctgca actcacctcc cggtcctaga gcaaagcacc
     1321 ctggatcaag gcaatgagat gctcaccggc gcgctgctgg ggtgtatcga ccagataaaa
     1381 cagggcgcga ccgtggtgca tgtggatgac caaactggct ggctggggtt catctgtata
     1441 cacgagcagc accaccatct ggcagtcaca cgcctgtgta ccatgataag ggcaggggca
     1501 atccggatgg ctgagccgcg ccgcttgcag gttaaaagta ggcataacct gcaagccagc
     1561 gttggtcagc ttctggacag cccagtcgac caggtgatcg cagggtttat ctaagatcaa
     1621 aaaaggcgag gcaccaatca tgcgttttca attaaggaat tatggcaccc agttggacag
     1681 tacagtcagg gtacgctccg gctgctgagg gtcgttgctg gctacatgga cccgaaaatc
     1741 gtgcattccg tccatatcac catgcatcat aaattccata gacagcgtgg tattttcacc
     1801 aggattgagg gtcatcgcac cgatgactag tttg
//
LOCUS       DBNHOH010000258         3699 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1681397, whole genome shotgun sequence.
ACCESSION   DBNHOH010000258 DBNHOH010000000
VERSION     DBNHOH010000258.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1681397"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="AC16V7_04555"
     CDS             complement(<1..658)
                     /locus_tag="AC16V7_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxymethylpterin diphosphokinase MptE-like
                     protein"
                     /protein_id="HIR6256423.1"
                     /translation="MNKQAFKQFFPPPLWRAASEMRYSLVRAAQWPAATFHPWRRESI
                     RQLEVLKDKHRGQRCFIIGNGPSLKKTDLSRLRGECTFGMNRIYLMFPELGFTTSYYL
                     SVNSLVIEQCADEISALPIPKFLSWRSRHLIQPSANMAFLFTTYTGPKFARDARGRLW
                     EGATVTYVALQLAYHMGFQQVILIGVDHSFTTQGKPNTTITSQGDDPDHFHAGYFGKG
                     F"
     gene            complement(704..1483)
                     /locus_tag="AC16V7_04560"
     CDS             complement(704..1483)
                     /locus_tag="AC16V7_04560"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009785906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6256424.1"
                     /translation="MTQTPANPPLKAVLITGAAGGIGRACVNIFAQMGWRVLGVDRSA
                     FGAGFPSGSLFVQSDISIGENLKTIFNQAHAFTGYLNALVNNAALQIAKPLLETSVEE
                     WDAVMASNLRSVFLGVKLAHPLLKAAGGGAIVNVSSVHAVATSANIAAYAASKGGLLA
                     LTRAMAIEFAPDNIRANAILPGAVDTPMLRAGLGRGHVGGDDILERLDNLARKTVNGR
                     IGTPEEIARAIYFLADDAQSSFMTGQALIIDGGATARLSTE"
     gene            complement(1549..2520)
                     /locus_tag="AC16V7_04565"
     CDS             complement(1549..2520)
                     /locus_tag="AC16V7_04565"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HIR6256425.1"
                     /translation="MYTILLTAPYMVPLYERFLPVFKHYGLEVIVVPVKERLDEEQLL
                     SYAGKFDGALAGDDQYTARVLEACAPRLKVISKWGTGIDSIDPQACTRLGIKIGRTPN
                     AFTLPVSDSVMGYVLAFARRQPWMDRDIKAGVWYKLPGRSLSETTLGVVGLGNIGKAV
                     IRRARPFGMRLLGVDIIEIAPDFLVENGVEMTSLEDLLARADFVSLNCDLNPTSYHLM
                     DARTLTLMKPTAVLINTARGPVVDEPALIAALQSQSIAGAALDVYEHEPLPPESPLRQ
                     MENVMLAPHNSNSSPAAWERVHWNTLRNLLEGLGQDASSLESFKASG"
     gene            complement(2579..3259)
                     /locus_tag="AC16V7_04570"
     CDS             complement(2579..3259)
                     /locus_tag="AC16V7_04570"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009785909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HIR6256426.1"
                     /translation="MTLVALVPMRHHSQRVPGKNYRPLGSKPLFHYIIESLLNCPEID
                     QVVVDTDSPPVMDGLRQHFPQVTLIERPEHLRADETPTNEVLLHDTAQVQADFYLQTH
                     STNPLLRPETISRAIQTFLSCYPAYDSLFSVTRWQTRLWDQLGRAINHNPAILLQTQD
                     LPPVYEENSCIYIFTRQNLVARRNRLGERPLLFEIGASEAWDIDEELDFAIVDFLMRQ
                     GIGDNGER"
     gene            complement(3266..>3699)
                     /locus_tag="AC16V7_04575"
     CDS             complement(3266..>3699)
                     /locus_tag="AC16V7_04575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR6256427.1"
                     /translation="GLEDLDWAHWAVGEGYRLAYVAEAEVIHVHNETPRAVYNRYRRE
                     AMAFKRIFPQEHFGLRDFLRLSLSNIFSDMWHAARQGVLFRSLGSIFWFRLMQFSGTY
                     QGYRHHGPLTWQLRQTFYYPRGIAPTGQAAARNVEPIRYNE"
BASE COUNT          759 a          998 c         1198 g          744 t
ORIGIN      
        1 gaaagccttt gccgaagtaa ccggcgtgga aatggtcggg gtcgtcgccc tgcgaggtga
       61 tggtggtgtt gggcttgccc tgagtggtaa agctgtgatc cacaccgatc aggatgacct
      121 gttgaaagcc catatgatag gctaattgca gggctacgta ggtgacagtg gcgccctccc
      181 acaggcggcc gcgggcgtcg cgggcgaact ttggcccggt gtatgtggta aacaggaagg
      241 ccatattcgc gctgggctgg atgagatgcc gcgagcgcca ggagagaaac ttcggaatgg
      301 gcaaagcgct gatctcatca gcgcactgct cgatcaccag gctatttacc gagagatagt
      361 aagaagtggt aaagccaagc tcggggaaca ttaagtaaat gcggttcatg ccaaaggtgc
      421 actcgccccg caggcgagaa aggtcggtct tcttcaggct gggaccgttg ccgataatga
      481 agcagcgctg gccgcggtgc ttgtctttga gcacttcaag ctggcggatg ctctcccgcc
      541 gccaggggtg aaaggtcgcc gcgggccatt gggcggcgcg cacaagcgaa tagcgcatct
      601 cgctggctgc gcgccagaga ggagggggaa agaactgctt aaaggcttgt ttattcatcg
      661 aaaatgaaga agagggtttt tcccctagcc tgcaatttgt ggatcactcc gtgctcaatc
      721 tggctgtggc accgccgtcg atgatcaggg cttgcccggt catgaaggat gattgtgcat
      781 catcggccaa aaagtagatg gcgcgggcga tctcttctgg agttccgatg cgcccgttga
      841 cggttttgcg cgccaggttg tccagacgct ccaggatatc atcgccgccc acatggccgc
      901 ggcccagccc ggcgcgcagc atgggcgtgt ccaccgcgcc cggcaagatg gcgttggcac
      961 ggatattgtc gggggcgaac tcgattgcca tggcgcgcgt cagcgccagc aggccgccct
     1021 tgctggcagc gtaggcggcg atgttcgcgg atgtagccac ggcgtgcacc gaggagacgt
     1081 tgacaatagc ccctccgcca gccgccttga gtaacgggtg ggcgagctta acccctagaa
     1141 aaaccgagcg caggttggag gccatcaccg catcccactc ttccacgctc gtctccagta
     1201 gaggcttggc aatctgcagg gcggcgttgt tgaccagggc gttcaggtag ccagtgaagg
     1261 cgtgcgcttg gttgaagata gtttttaaat tctcgccaat agaaatatca gactggacga
     1321 aaaggctgcc agaggggaag cccgcgccga aagcggagcg atccacaccg agcacgcgcc
     1381 agcccatctg ggcgaagatg ttcacgcacg cgcgcccgat accaccagcc gcgccggtga
     1441 ttaggacagc ctttagaggt gggttagcag gtgtttgtgt catgtcactt taagcctacg
     1501 agttgtaatg cggttgtcgg tcgggggata acggtcaact gtttactttc aaccgcttgc
     1561 tttgaaagat tccagagaac tggcgtcttg ccctaagcct tccaatagat tgcggagggt
     1621 gttccaatgg acacgctccc aggctgccgg gctggaattg gagttatgcg gcgccagcat
     1681 gacattctcc atctgcctga gcgggctctc aggcgggagg ggttcatgct cgtatacgtc
     1741 cagggcagct ccggcgatgg attggctttg caaggcagcg atcagggcgg gctcatctac
     1801 taccgggccg cgagcggtgt tgatcagcac ggctgtgggc ttcattaatg tcagggtgcg
     1861 cgcatccatc aggtggtagc tggtggggtt caggtcgcag ttcaagctga cgaaatcggc
     1921 tcgagccagc aggtcttcca ggctggtcat ctcaacgccg ttctccacca gaaagtctgg
     1981 tgcgatctcg atgatgtcaa cccctagcag gcgcatgccg aaggggcgcg cccggcggat
     2041 gaccgccttg ccgatattgc ctaatcccac cacccccagg gtggtttcgc tcaaagatcg
     2101 cccggggagt ttataccata ccccggcctt gatgtcgcgg tccatccagg gctgtcggcg
     2161 agcgaaagct aggacataac ccatcacgct atcggagacg ggcagcgtga atgcgttggg
     2221 agtgcgcccg atcttgatcc ctagacgggt gcaggcctgc gggtcaatag agtcgatacc
     2281 ggtgccccat ttggagatca ctttcaaccg cggggcgcag gcctccagga cgcgggcagt
     2341 atactggtca tcgcctgcca gagcgccatc gaactttccg gcgtaggaca ggagctgctc
     2401 ctcgtcgagc cgttccttca ctggtacaac gatcacctcc aacccataat gtttgaatac
     2461 cgggaggaaa cgttcataca gagggaccat atagggagca gtgagcagga tggtgtacat
     2521 aagggactag gggtaaggga gtaggggaga ggggataaga aggtgggaaa cggagggatt
     2581 acctctctcc gttatcgcct atcccctgtc tcattaagaa gtctacgatg gcgaaatcca
     2641 actcctcgtc gatgtcccag gcctcggaag cgccgatctc gaagagcaag gggcgctctc
     2701 ccaggcggtt gcggcgggca accaggttct ggcgagtgaa gatgtatatg caggagttct
     2761 cctcataaac cgggggcaga tcctgggttt gaagcaggat cgccgggtta tggttgatcg
     2821 cccgcccaag ctggtcccac aggcgggttt gccagcgggt gacggaaaag agcgaatcgt
     2881 aggccggata gcaagaaagg aaggtctgga tggcgcgcga gatcgtctct gggcgcagca
     2941 gggggtttgt gctgtgcgtc tgcaggtaaa aatcggcttg cacctgggcg gtgtcgtgca
     3001 gcagcacttc gttggtgggg gtctcgtcgg cgcgcaggtg ctcggggcgc tcaatgaggg
     3061 ttacctgggg gaaatgctgg cgcagcccgt ccatcacggg cgggctgtcg gtatccacca
     3121 cgacctgatc gatctctggg cagtttagaa gactctcgat aatgtagtgg aagagcggct
     3181 tgctgccaag cgggcggtag ttcttacccg gcacgcgctg cgagtggtgg cgcataggga
     3241 cgagggcgac gagggtcata gattattact cattataccg aataggttcc acattccgcg
     3301 cagctgcctg gccggttggc gcaatacccc tgggataata aaatgtctgc cggagctgcc
     3361 aggtgagcgg cccatggtgg cggtagccct gataggtgcc cgagaactgc atcaggcgaa
     3421 accagaagat gctccccagg ctgcgaaaca agacaccttg ccgtgcggcg tgccacatat
     3481 cgctgaatat gttgctcagg cttaggcgca ggaagtcgcg cagaccgaaa tgctcctggg
     3541 ggaagatgcg tttgaaggcc attgcctccc ggcggtatcg attgtacacc gcgcgtggcg
     3601 tctcgttgtg cacgtggatc acctccgctt cggctacata ggccagcctg tacccctcgc
     3661 ccactgccca gtgtgcccaa tccaggtcct ctagtccgc
//
LOCUS       DBNHOH010000259         1618 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1168272, whole genome shotgun sequence.
ACCESSION   DBNHOH010000259 DBNHOH010000000
VERSION     DBNHOH010000259.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1168272"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="AC16V7_04580"
     CDS             <1..1087
                     /locus_tag="AC16V7_04580"
                     /inference="COORDINATES: protein motif:HMM:NF018012.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR6256428.1"
                     /translation="LIDYFRLMDGLEHIDFIAPVYARDFPEANRDVKVFELMIANTDK
                     HMHMRAYSKKSLEIILEMATIVAGSKESLKKRPVLSLLEAPLSPLTFLDITVDALFLC
                     GEFGIPLELCCMPIAGGTGPVTLAGSVTLANAEFLAGVVISQLYRPGAPLEYAPRCMI
                     MDMKTTVGLTGSIEGAMMAAAGAQLAREKYNAPISLHGPWTDSVLPDEQSNLERTNFA
                     FLAGLAGTNVFSGAGMIEQGKTFSHLQLVMDDEIHAMVKLVLGGMSTTQDHLAVEAIE
                     RAGPGGNFLIDPLTLKYFKTERFFPSLLLRETRSSWVSKGGKSYRERAREKVFEILKH
                     HQPNPMEEEVMNEISAIVKRSEKELEG"
     gene            1096..>1618
                     /locus_tag="AC16V7_04585"
     CDS             1096..>1618
                     /locus_tag="AC16V7_04585"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR6256429.1"
                     /translation="MVTRKMLFSPGPVLTSERVKASLIHPDLCHRRPDFEQVLHRVRE
                     NILRLFNADHEYTSVVISGSGTAANETALSSIIKDADEVLLIKNGEFGERLHDILNCY
                     RLRVHVLEYPWGSPPELSDVRRYLVGNENIQWVCMVFHETSTGMLNPVRQVGELAHEF
                     QRRFYVDCVSAVGG"
BASE COUNT          406 a          380 c          443 g          389 t
ORIGIN      
        1 tctaattgat tatttccgcc tgatggatgg gttggaacac atcgatttta tcgcaccagt
       61 gtacgcacgg gattttccgg aagcaaaccg tgatgtgaag gtgtttgaat tgatgatcgc
      121 caacaccgat aaacatatgc acatgcgcgc ctatagcaag aagagccttg agatcatcct
      181 cgagatggct actatcgtgg cagggagcaa agaaagtctg aaaaaacgcc cagtattaag
      241 cttgctagaa gcaccgctca gcccgctcac tttcttggat atcactgtag atgctctatt
      301 cctgtgtggt gaattcggca tcccgctgga attgtgctgt atgcccatcg ctggtggcac
      361 aggtccggtg acactggcgg gtagtgtgac cctggcaaac gccgagttcc tggccggcgt
      421 ggtaatctcg cagttatatc gcccaggtgc gcctctcgaa tatgctcccc gttgtatgat
      481 catggacatg aagactaccg tgggattaac cggttccatc gaaggtgcga tgatggccgc
      541 tgcgggtgct caactggcgc gcgaaaagta taacgcgccg atcagcctgc atggcccctg
      601 gacagactct gtgctcccgg atgagcaatc gaatctcgag cgaacaaatt ttgccttctt
      661 ggctggtcta gctgggacaa acgtcttttc tggagctggc atgatcgaac aaggaaaaac
      721 cttcagccat cttcagcttg tgatggatga tgagattcat gctatggtca agctggtatt
      781 aggtggtatg tccactacac aggatcatct agccgtggag gcaattgagc gagctgggcc
      841 aggaggaaac ttcttgattg acccactgac actaaaatac ttcaagaccg agcgcttctt
      901 cccaagcctg ctattacgcg aaacaaggtc ttcctgggta tcaaaaggcg gtaagtctta
      961 tcgagaacgt gctcgggaga aggtgtttga gatcttgaaa catcaccaac ccaatcccat
     1021 ggaggaagag gtgatgaacg aaatatcggc aattgttaag agatccgaga aagagctaga
     1081 aggttgatca taaatatggt tacaagaaaa atgttgttca gcccgggtcc cgttctaact
     1141 tcggagcggg tgaaggcatc tctgatccat ccagacttat gccaccggcg tccagatttt
     1201 gagcaagttt tacaccgggt gcgcgaaaat attttgcggc tgtttaatgc cgaccatgaa
     1261 tacacttcgg tcgtgatcag tggttctggc accgcagcca acgagacagc actatcgtcg
     1321 atcatcaagg acgctgacga ggtcttgctg ataaaaaatg gcgagttcgg cgagcgatta
     1381 cacgatatct taaattgtta ccgtttgcgt gtacacgtcc tggaatatcc ctggggcagc
     1441 ccgccggagc taagcgacgt ccgcaggtat ctggtgggga acgagaatat acaatgggtt
     1501 tgcatggttt tccacgaaac cagcacagga atgctaaacc ctgttcgcca ggttggcgaa
     1561 ttggcgcatg agttccagcg aagattttac gtggactgcg tcagcgcggt cggggggg
//
LOCUS       DBNHOH010000260         6508 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1040705, whole genome shotgun sequence.
ACCESSION   DBNHOH010000260 DBNHOH010000000
VERSION     DBNHOH010000260.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6508)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1040705"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="AC16V7_04590"
     CDS             complement(<1..1061)
                     /locus_tag="AC16V7_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR6256430.1"
                     /translation="MLTLAEKILFVIAILASLIAAYRVADRIIRTIRRGQGEVDWLIA
                     QKRLIGVLVKTIALQPVFRLRFWPSLFHGLVAWGFIYYLLVNLFDVLAGYIEGFAIPG
                     VLGDLYRLGADLLSVGALVGMLALIIRRFGMKPATLSARQDVLLHPKARAGIRRDSAI
                     VGGFILVHVGSRFFGQSVHIAATGHDAWQPFASLVAKLWAGWSDAALTFGEHAAFWLA
                     LGTILAFIPYFLYSKHIHLFFAPINFLLKPERLSIGELGRLDFDDESIEQFGATRLED
                     LGWEQIMDAYACIMCYRCQEVCPAYNTGKVLSPAALEINKRYFLNYEGERMAKGEPSE
                     KTLLEFAIPAEAVWACTACG"
     gene            complement(1100..2497)
                     /locus_tag="AC16V7_04595"
     CDS             complement(1100..2497)
                     /locus_tag="AC16V7_04595"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF015531.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR6256431.1"
                     /translation="MKPRSHAVWILPFVLVLLACQAVTGLVQPTATTSLLHTSKNLPK
                     PVSATPTSTETPLPTRTATPTETPTPTLTATATIVPSPSALQLKVFEELWHAVLTDYL
                     YPDYNGQDWEAVGEKYRQRIKDGMSAHDFYNAMEEMIASLNDEHSVFLRPEEAIEEDA
                     EFSGANDYVGIGVLTNVVPERKRAVILIVFPNSPAEEAGLKSHDSILAVDGEPLIDET
                     GMRRNLLRGPENTSLELTVQTPGEAPRQISLTRRRVTGSVPVPYAVFTTPHGKRVGYI
                     LLVTFSDETIDNQVEKALKALTAQGRLDGLILDNRVNAGGADNVARAVLGFFTKGTLG
                     YFYRRDQSRRAFNVIGTEINGSYEVPLVVLVGPDTVSFGEIFSGVLKDNGRAYVIGEL
                     TQGNVELLWGYNFEDGSRAWLAQEKFRPRNNPDQDWEETGIIPDLTVATNWDEIVPDT
                     DPAILAALEYMDGLE"
     gene            complement(2584..3789)
                     /locus_tag="AC16V7_04600"
     CDS             complement(2584..3789)
                     /locus_tag="AC16V7_04600"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HIR6256432.1"
                     /translation="MYSILGLFGKIGARRSHRWALGLILLAALISYRPALAAGDDPGV
                     LCLPDVYLQVASDCTPLGPSAYLTHLAEKNFTFTAAPLTYKRPDFSLTNVNTRYGQVN
                     KTNAPVYGSLEDALKGNKKEATRQIDAVFSYISYTEDVVVDGKRFYMIDQDAWMTAND
                     IVRLGAVPLFQGLTFNHTPNHSFGWVLSYLSQGPVETKRTPGWQNQDYTGHTLNNHEI
                     VPVYSTEVVNEEEWYMVGPDEWLHQNVVARVVPNPTPPQGVTGDRWIEINLFEQTLAI
                     YEQRQLVFATLIASGLDPFWTRPGLFQIYDKLDTTPMRGAFERDRSDAYYLEDVPWTM
                     YFDQARALHGAYWRANLGFPQSHGCVNLSVGDSRWIFNWANIGDWVYVWDPSGKTPID
                     PSVYTAGGA"
     gene            complement(3782..4642)
                     /locus_tag="AC16V7_04605"
     CDS             complement(3782..4642)
                     /locus_tag="AC16V7_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013615246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     polyprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HIR6256433.1"
                     /translation="MKSVRDFLELIKFEHTVFALPFAYLGMLLAARGWPGWKEFIWIT
                     IAMAAARTVAMGFNRIADRWIDAHNPRTAKRPLVTGMISTRVAWGGTFVAAAVFTLAA
                     WQLGPLPFKLLPGAFLFLFGYSFTKRFTWLSHYIVGFTDGLAPMGAWAAVRGSLFTQA
                     DLPAWIMLGVVTLWIGGFDLIYACQDVPYDQQDGLHSVPARFGIPTALRLSTVSHILT
                     VGLLAILGVLMGLGWPYWIGLLLVAGLLTWEHTLVHPDDLSRVDMAFFNLNSYISVTL
                     FLSILGALYV"
     gene            complement(4742..5299)
                     /locus_tag="AC16V7_04610"
     CDS             complement(4742..5299)
                     /locus_tag="AC16V7_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR6256434.1"
                     /translation="MRIHQSSFLRHTFAILIILAMVLGAFPNAAQAATCDTYYTVKSG
                     DTKTKIAKLYKIKWREIAEANDLEEPYNLKVGQKLCIPYNADDDEELANPKLRLRVSA
                     THTIVTLTLSGLSDKKAVFIVRMRDATIGIGGWQKLGRIKLKKNSTSKETFRIPKEFK
                     NTLYLRVCIKNSSTDELTCQVVLHP"
     gene            complement(5351..6091)
                     /locus_tag="AC16V7_04615"
     CDS             complement(5351..6091)
                     /locus_tag="AC16V7_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6256435.1"
                     /translation="MKKRDLLFAVAALIFLWQLVAMAVNRPILPTPWVVTQTFVDELR
                     SDLLVHFRASLWRVIASTLLAIALAAPAGLVLGQSKKLNSFFSPLIYLVYPVPKVVFV
                     PIVLLFLGLGDSPKIVIIFLILFFQVLVLVRDQAAALRPELIQSVRSLGAGRRALFRF
                     VYLPASLPAILTALRQSVGTAVAVLYVAELYATQKGLGYYIYLNGSTLLNYPAMYAGI
                     VAMSLLGLGLYFSVDWLEKRLCPWLFTS"
     gene            complement(6108..>6508)
                     /locus_tag="AC16V7_04620"
     CDS             complement(6108..>6508)
                     /locus_tag="AC16V7_04620"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6256436.1"
                     /translation="LERLGLSSVTNKYPAQISGGQRQRTAIARTLALKPDLLLMDEPF
                     SSLDAPTRESLQNLTLELQSEQNLTLVIVTHAIEEAAIMGRKILLLGTPPNREAQVVE
                     NPGATQSNFRETAQYLEMCKHLRSLMETPT"
BASE COUNT         1598 a         1707 c         1782 g         1421 t
ORIGIN      
        1 ccgcaagccg tgcatgccca aactgcttcg gctgggatag caaactccag cagagtcttc
       61 tcgctcggct cgcctttagc catccgttcg ccttcgtagt tcagaaagta gcgcttgttg
      121 atctccagcg cagctgggga gagcaccttg cctgtgttgt aggccgggca gacctcctgg
      181 cagcggtagc acattatgca ggcataggca tccataatct gctcccaacc caggtcttcc
      241 aggcgggtgg ctccaaattg ctcgatgctt tcgtcgtcaa agtccaaacg accaagctcc
      301 ccaatggaga ggcgttcagg cttaagcagg aagttgatgg gggcaaaaaa taagtggatg
      361 tgcttggagt acaggaaata gggaataaat gccaggattg tgcccaaagc aagccagaaa
      421 gcggcgtgtt cgccgaaggt taaagccgca tcgctccacc cagcccacag cttggctaca
      481 aggctggcaa agggttgcca ggcatcatgt cccgtggcag cgatatgcac ggattggccg
      541 aagaagcgcg accccacatg caccaaaatg aatccaccga cgattgccga atcccgtcgg
      601 atgccagcgc gcgctttagg gtgcagcagc acatcctggc gggcgcttaa ggtcgcgggc
      661 ttcatgccga atcggcggat gatcagggca agcataccaa ccagggcccc tacgctgagt
      721 aggtccgccc ccaagcggta caggtctcca agcacacctg ggatggcgaa gccctcgata
      781 taaccagcca gaacatcgaa caaattgacc agcagataat agataaaacc ccaggctacc
      841 aagccatgga ataagctggg ccaaaaacgg aggcgaaaga ccggctgcaa ggcgatggtt
      901 ttgaccagca cgccgatcag gcgtttttga gctatgagcc aatccacttc tccctgcccg
      961 cgtcggatgg tgcgaatgat gcggtctgca actcgatatg ctgcgatgag cgaggccagt
     1021 atggcgatga caaacaggat tttttcggcg agtgtgagca tgatgcctcc gctttctcgg
     1081 tgtcgggagt aggcgtattt tactctaatc cgtccatata ctccaatgct gccagaattg
     1141 cagggtcagt gtctggaacg atttcatccc aattcgtggc tacagtcagg tcggggataa
     1201 tgccggtctc ttcccaatcc tgatcggggt tgtttcgagg gcggaatttc tcttgggcaa
     1261 gccaagcgcg tgagccgtct tcgaagttat aaccccaaag tagctcaaca ttaccctgtg
     1321 tcagctcccc aatgacatag gctcgtccat tgtccttcaa cacccctgag aatatctctc
     1381 cgaagctcac cgtgtctggt cctaccagca cgaccagggg cacttcatac gaaccattga
     1441 tttcagttcc aatcacgtta aaggcgcgcc tggattggtc ccggcgatag aaatatccta
     1501 atgtcccttt cgtaaagaaa cccaaaacgg ctcgggcgac gttatctgca ccaccagcgt
     1561 tgacacggtt atccaggatc aagccatcca gacggccttg tgcagtcagc gctttcagcg
     1621 ctttctcgac ctggttatct atcgtctcat ccgaaaaggt aacgagcaga atatagccaa
     1681 cgcgtttgcc gtgtggagtg gtaaatacgg cataaggcac cggcactgag ccggtcacgc
     1741 gccgccgggt gaggctgatc tggcgcggag cttcccctgg ggtttgtaca gtcaattcga
     1801 ggcttgtgtt ttcaggaccg cgcagcaggt ttcgccgcat cccggtctcg tcaataagcg
     1861 gctcaccatc cacagccaaa atgctgtcgt gggatttcag gccggcttct tctgcggggc
     1921 tgttgggaaa cacgatcaaa atgactgcac gcttgcgctc tggaaccaca ttggtaagca
     1981 ccccgatgcc cacatagtcg ttggcaccag aaaattcagc atcttcctca attgcctcct
     2041 cagggcgcaa gaaaaccgag tgctcatcgt tcaggctggc aatcatctcc tccatcgcat
     2101 tgtaaaaatc atgggcgctc atgccatcct taatgcgttg acggtatttc tctcccaccg
     2161 cctcccagtc ttgaccattg taatcagggt acagataatc agtcagaaca gcgtgccaga
     2221 gctcctcaaa aaccttgagt tggagagccg aggggcttgg gacaatggtt gcagtagctg
     2281 taagggttgg tgtaggtgtt tcggtggggg tagcagttct agtcggaagg ggtgtctcag
     2341 tagaggttgg tgtggcagag accggcttgg gcaggttttt gctggtatgc aatagcgagg
     2401 tggtcgcggt gggctgcacc aaaccggtga cagcctggca agccagcaaa actagcacaa
     2461 aagggagaat ccaaacagca tgcgagcgtg gtttcatgtg cagcagcggt cacgaaccag
     2521 agaaatggtg cttacgttgc agccaggaag cagacaattg tcaataaaag ccgggaggca
     2581 gccttacgcg ccaccagcag tataaaccga tggatcaatt ggtgtcttac ccgaagggtc
     2641 ccaaacgtac acccaatcgc cgatgtttgc ccaattgaag atccagcgag aatcacctac
     2701 tgataggttg acacagccgt gtgattgagg gaaacccagg ttggcgcgcc aatatgcacc
     2761 gtgcagggcg cgcgcttggt caaaatacat cgtccaaggg acatcttcga gataatatgc
     2821 gtcagagcgg tcacgctcaa aggccccgcg catgggtgtt gtatccagtt tatcgtagat
     2881 ttgaaacagg cccgggcgcg tccagaaggg gtccaggccg ctggcgatca gtgtagcgaa
     2941 gaccaattgg cgttgctcat atatggccag ggtttgctca aacaggttga tttcgatcca
     3001 gcggtcacca gtcacacctt ggggcggagt agggttaggc acaacacgag ccaccacgtt
     3061 ctggtgcagc cattcatccg gcccaaccat gtaccactct tcctcattga cgacctctgt
     3121 ggagtacacc gggacgatct catgattgtt gagtgtatgg ccagtgtaat cctgattctg
     3181 ccagccaggt gtgcgcttgg tctcaaccgg tccctggctg aggtaggata gaacccaacc
     3241 aaatgaatgg ttaggcgtgt ggttgaatgt aagcccttgg aataggggca ccgcaccaag
     3301 gcggacaata tcattggctg tcatccaggc atcttggtcg atcatataaa agcgcttgcc
     3361 atccactacc acatcttcag tataggagat ataactaaat acggcatcaa tctgcctggt
     3421 agcttctttt ttattgccct tcagagcatc ctctagggaa ccatataccg gcgcgtttgt
     3481 tttattgact tgaccataac gcgtattgac gttggtcaag gaaaaatccg gacgtttata
     3541 tgtgagcggg gcagcagtaa aggtgaagtt tttctcggct aggtgagtca gatacgccga
     3601 gggaccaaga ggggtgcaat cgctggcgac ttgcaggtag acgtccggca ggcagagcac
     3661 accaggatca tcacctgccg ccagggcagg tcgatatgaa ataagggcag caagcaagat
     3721 caagcccagc gcccaacgat gagatcgccg cgcgccaatt tttccgaata aaccgagaat
     3781 actatacata taaagcacct aatattgaaa gaaaaagcgt aacactaata taactattga
     3841 ggttgaagaa ggccatatcc acccggctga ggtcatccgg atggaccagg gtgtgttccc
     3901 aggtcagcag tccagccacc aagagcagac caatccaata cggccagccg agtcccatca
     3961 aaactcccag gattgccaag agcccaacgg tgagaatatg actgactgta gacaggcgta
     4021 aggcggtggg gatgccaaaa cgggcaggga ctgaatgcag gccatcttgc tggtcatagg
     4081 gcacgtcctg gcaggcatag atgagatcga agccgccaat ccacagcgtg accacaccga
     4141 gcataatcca ggcgggtaga tcggcctggg tgaatagcga accgcgcacc gccgcccagg
     4201 ctcccattgg agctaagccg tccgtgaaac caacaatgta gtgggaaagc catgtgaagc
     4261 gcttggtgaa tgagtacccg aacaggaata gaaacgcgcc gggcaataac ttgaacggta
     4321 gcgggcctag ttgccaggct gccagggtga aaacagccgc tgcgacaaaa gtcccacccc
     4381 aggcaacgcg tgtagatatc atgccggtga ccagggggcg tttggctgtg cgcgggttgt
     4441 gggcgtcgat ccagcggtcg gcgatgcggt tgaaacccat cgccaccgtg cgcgctgcag
     4501 ccatcgcaat cgtgatccaa ataaattcct tccacccggg ccagcctctg gcagccagca
     4561 gcatacccag gtaggcgaac ggcagcgcaa agacagtgtg ctcgaatttg atgagctcga
     4621 ggaaatctcg tacagatttc atgggatgct attatagcgt agaacggaaa attgggtgat
     4681 cgcggcgttg tagctgcctg ttcacccaat tcttcgggtt cctttccgtt ggagtcaaat
     4741 attacggatg caagactacc tggcaggtta gctcatcggt ggaggagttt ttaatacaga
     4801 cacgcaggta aagcgtgttc ttgaactctt tggggatacg gaaggtctct ttggaggtcg
     4861 agttcttctt gagctttatc ctgcccagtt tttgccatcc gccaatgccg atcgtggcat
     4921 cacgcatgcg cacaataaaa acggcttttt tatccgacaa acccgatagc gtaagtgtca
     4981 ctattgtatg agtggcactg accctcaatc ttagtttggg gttggctagt tcttcgtcgt
     5041 catcagcatt gtacgggatg cagagcttct gccctacttt gaggttatat ggttcttcca
     5101 gatcgttcgc ctcagcgatc tcccgccatt tgatcttata caatttcgcg atcttggttt
     5161 tcgtatcacc tgacttgaca gtatagtacg tgtcgcaagt ggcagcttgg gctgcattcg
     5221 ggaacgcccc caacaccata gcgagtatga tcaggatcgc gaacgtatgc ctaaggaacg
     5281 atgattgatg gatcctcatt tattactcct ttctttgatc ccggacctcc tgtggagatt
     5341 atacactaaa tcagcttgtg aacagccacg ggcagaggcg cttctcaagc cagtccacgc
     5401 tgaaatacag tcccaatccc agcaggctca tcgctacaat ccctgcgtac atagcggggt
     5461 agtttaacag cgtgctgcca ttcagataaa tataataacc caggcctttt tgggtggcgt
     5521 acaactcagc cacataaagc acagccactg ctgtgcctac cgactgtcgt aaagccgtca
     5581 atatcgccgg aaggctcgcc ggcagataga caaagcgaaa taaagcccgc cgccctgctc
     5641 ccaggctgcg cacgctctga attaattctg gtctcagggc agccgcctgg tcacgtacca
     5701 acaccaatac ctggaaaaat aaaattaaaa aaataatcac gatcttggga gaatcgccca
     5761 ggccgaggaa aaggaggaca ataggcacga agactacctt cggcacaggg tagaccaggt
     5821 aaatgagcgg agagaaaaag ctgttcagct tctttgactg gcctaagacc agtcctgcag
     5881 gagccgccag cgcaattgcg agcagcgtac tggcaatcac ccgccaaagg ctcgcccgga
     5941 aatggaccag caggtcgctg cgcagctcat caacgaaggt ttgcgtcacg acccacggcg
     6001 tgggcaagat tggccggttc actgccatgg caaccagctg ccagagaaag attagtgcag
     6061 cgacagcaaa gagaaggtct cttttcttca ttacaggttt acctctctca ggttggtgtc
     6121 tccataagag agcgcaagtg cttgcacatt tccaggtatt gtgcagtctc tcgaaagttt
     6181 gattgggttg caccagggtt ttccaccact tgagcttctc gattgggagg ggtgccgagg
     6241 agcaggattt tgcgccccat gatggctgct tcttcgatag cgtgggtgac gatcaccagg
     6301 gtcaaatttt gctcggattg caattcaagg gtcagatttt gcaaactttc gcgtgtgggg
     6361 gcatccagtg aggagaaagg ctcgtccatc aggagcaggt ctggcttgag cgccagggtg
     6421 cgagcgatgg ctgtgcgctg gcgctgacca cctgagattt gagcggggta cttattggtc
     6481 acagagctga gacccagtct ctccaacc
//
LOCUS       DBNHOH010000261         3668 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_463837, whole genome shotgun sequence.
ACCESSION   DBNHOH010000261 DBNHOH010000000
VERSION     DBNHOH010000261.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_463837"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="AC16V7_04625"
     CDS             <1..355
                     /locus_tag="AC16V7_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256437.1"
                     /translation="LLRATLYPPLMTAYPQASETQQEILMVVFQGVTNFNFEGLWALE
                     EIFWGIWWLGIGLVLRSERRSLGIFTVILGIAFLGGGAGWLLRVGPLARLENAFFLVP
                     FWAVWLGVVIWRRGE"
     gene            506..2554
                     /locus_tag="AC16V7_04630"
     CDS             506..2554
                     /locus_tag="AC16V7_04630"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6256438.1"
                     /translation="MTQRTARWIAWFLVVFYVNLATVGLALQAITDTPFVKSGLPVLI
                     FLVILVGMWFVTGALIISRHPQHPVGWLLCAGLITGAIDMFAAGYAAYDTYVFSGTLV
                     GVDLALIWLKLVNLGPHGLVAFTLIVLLFPDGRFPSPGWRKVAWTTVGTLLFFLPLQA
                     LEPGPADPFILPMRANPLGVSASQWSFLEPLMWTAFSILVLCYGAAFVSLIVRLRNSR
                     GDVRQQIKWLLFPAGLFGIYLVVFIIGMTEADQGIMEIGIALGQLAIAGMVIAIAFAI
                     FKYRLYDIDLLINRTLVYGTLTACVVGLYVLTVGALGMFFQTQGNLIIALLATGIVAA
                     LFQPLRERLQRGVNRLIYGERDDPVEALSRLGRQLEMAVPPDKVLPTLVETIAQTLKL
                     PYVAIYLPHQEGDKIAAIYGNSVPELVQFPLVYQGEDTGQLAVGLRSPGSPFSPAEMR
                     LLQNIALQAGTAVHAVQLTTDLQRSRQNLVAAREEERLRLRRDLHDGLGPAIASVIWQ
                     ADSAREMIHTNPSEAAHLLERSIEQAQAALADIRRLVYGLRPPALDELGLVGALEQSA
                     RQHQHTSVTIEAPAPFPSLPAAVEVAAYRIIQEALKNAIEHGKAKNCVTSISFDDGLA
                     PGSLCFTIRDDGVGLPEVVTPGVGLISMRERTEELGGAFTIHPRQAGGTEVEVRLPLD
                     "
     gene            2565..3233
                     /locus_tag="AC16V7_04635"
     CDS             2565..3233
                     /locus_tag="AC16V7_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018658321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6256439.1"
                     /translation="MENNTIHLLITDDSQAALTGLKALLKGVADIQLVGDAPGGGEAI
                     RLAERLQPDVILMDLHMPDMNGIEATRQIVHTSPHIAVLVLTMYDDDDSVFAAMQAGA
                     RGYLLKGASKKEMLRAIRDVASGAAIFSPAIARRMMSYFNQIRKQPAAHAFPELTQRE
                     REVLTLMAQHHSNQEIAQSLSLSDKTVRNYTSNIFAKLQVANRAEAIIQARDAGLGQE
                     VDPR"
     gene            complement(3438..>3668)
                     /locus_tag="AC16V7_04640"
     CDS             complement(3438..>3668)
                     /locus_tag="AC16V7_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256440.1"
                     /translation="SFQRRKRLMERTGPLLPLWTKEVPKARDNVLNLWEILASCQLGA
                     EGRPPNGARAWLLGPYSADGKIASLSGRRRSY"
BASE COUNT          749 a         1000 c         1028 g          891 t
ORIGIN      
        1 gctcctgcgc gccaccttat acccgcctct gatgactgct tatccacaag cttcggagac
       61 ccaacaggag atactcatgg tcgtctttca gggagtcaca aatttcaatt tcgagggttt
      121 gtgggcgctt gaggagatat tttggggcat atggtggctg ggcatcggtc tggtattacg
      181 gagcgaacgg cgcagcctgg gcatctttac ggtgatactg gggatcgcgt tccttggcgg
      241 cggcgctgga tggctcctga gagttgggcc ccttgccagg ctcgaaaatg cgttcttttt
      301 ggtgcctttc tgggcagtat ggctcggtgt tgtgatatgg cggcgaggtg agtagagcga
      361 gtagacggtg tcagtggcaa cggctgcgtg acatttttaa tgaaggagaa tcgaatgaat
      421 catctgtata atataatcac gtaaacgata aattttgtcg ttcttgatgg gcatcctcag
      481 aaatcgtcag aaggtgagtg atcgcatgac tcagcgtacg gcccggtgga tagcctggtt
      541 cctggtagtc ttttacgtca acctggcgac agtcggcctg gcactgcagg ccattaccga
      601 cacgccattt gttaaatcag gacttcccgt attaatcttc ctggtgattt tagtcggcat
      661 gtggtttgtc accggcgcac tcatcatctc acgccatccg cagcacccgg ttggctggct
      721 gctttgcgcc ggtttgatca ctggtgccat cgatatgttc gccgccggtt atgccgccta
      781 tgacacctat gtgttttccg gcacgctggt gggagtggac ctggcgctca tctggctcaa
      841 gctggttaac ttgggacctc atggattggt tgcctttacc ctgatcgtcc tgctgtttcc
      901 agatgggagg tttccctcgc ccggctggcg caaagtggcc tggacgaccg tcgggacgct
      961 gctcttcttt cttcctttgc aggcattaga gccaggtccc gccgatccat tcatcttgcc
     1021 gatgcgtgcc aatcctctcg gggtcagcgc atcccagtgg tccttcctgg aaccgttgat
     1081 gtggacggcg ttttccatcc tggtgctgtg ttatggagcc gcgtttgtat ccctgatcgt
     1141 gcggctgcgt aattcacgtg gcgatgttcg gcagcagatc aagtggctgc tcttcccggc
     1201 tggtttattt gggatctatt tggtggtgtt tattattggc atgactgagg ccgatcaagg
     1261 aatcatggag ataggcatcg ccctagggca gttggccatc gccggcatgg tcatcgccat
     1321 cgcattcgcc attttcaagt accggctcta cgacattgac ctgcttatca atcgcacgct
     1381 ggtctatggc acactgaccg cctgcgtggt tggcctgtat gtactgaccg ttggcgcatt
     1441 agggatgttc ttccaaactc agggcaacct gatcatcgcc ttattggcaa ccggtatcgt
     1501 agccgccctc ttccaaccgt tgcgagaaag attgcagcgt ggagtcaatc ggctcattta
     1561 cggggagcgg gatgatccag tggaagcgtt atccaggttg ggccggcagt tggaaatggc
     1621 tgtgccgccc gacaaagtgc tgcccaccct ggtagaaacc attgcccaaa ccctgaagct
     1681 gccttatgtt gccatctact taccgcatca ggaaggggat aaaatcgcag ctatatacgg
     1741 aaattccgtc ccagaacttg tccagttccc ccttgtctat cagggggaag ataccggcca
     1801 attggctgtt ggcttgcgca gcccgggcag cccattcagc ccggctgaaa tgcggctgct
     1861 gcagaacatt gccttgcagg cgggcacagc ggtccacgcc gtccagctaa ccactgatct
     1921 ccagcggtcg cgccagaatc tcgtcgccgc ccgtgaggaa gagcggcttc gcctgcgccg
     1981 cgacctgcac gacgggttgg gtccagccat cgcctctgtc atctggcagg ctgacagtgc
     2041 tcgtgaaatg atccacacga atccgtcaga ggctgcgcac ttgctggaaa gaagtatcga
     2101 acaggcgcaa gctgccctgg ccgacatccg gcgtctggtt tacggtttgc gaccgccggc
     2161 actggacgaa ttggggctgg tcggcgcttt ggagcaatcg gcccggcaac accaacatac
     2221 ttccgtcacc atcgaagcgc ccgcgccctt tccttcgctt ccagccgccg tggaagtcgc
     2281 agcctatcgc atcatacagg aagcgcttaa gaatgccatc gagcatggga aggctaagaa
     2341 ttgtgttacc agcatatcct ttgatgatgg ccttgcccct ggcagcttat gctttacgat
     2401 tcgcgacgac ggtgtggggt tgccggaagt agttacacca ggtgtggggc tgatctcaat
     2461 gcgtgagcgg acggaggagc tgggaggcgc gtttacgatc catccacgac aagccggcgg
     2521 aacagaggtc gaggtgcgat taccgctgga ttaagagagt gcccatggaa aacaacacca
     2581 tccatctgct cataactgac gattcccagg ccgcactgac tggattgaag gcactcctga
     2641 aaggagtggc agacattcaa ctggtaggcg acgcgcctgg cggtggcgaa gccatccggc
     2701 ttgctgaacg gctacagcct gatgtcatcc tcatggacct gcacatgccg gatatgaatg
     2761 gcattgaggc cacgcgccag atcgtgcaca ccagcccgca tatcgccgtt ctcgtcttga
     2821 cgatgtacga tgacgacgac tctgtgttcg cagcgatgca agccggtgcg cgaggttatc
     2881 tgcttaaagg agcttccaag aaagaaatgc tgcgtgcgat ccgggatgta gccagcgggg
     2941 ccgcaatctt cagtccagct atcgcacggc gcatgatgag ctactttaat cagatccgca
     3001 aacagccagc cgcgcacgct ttccccgaat taacccagcg tgaaagggag gtactgacgc
     3061 taatggccca gcatcactcc aatcaggaaa tcgcccagtc gctctcactg agcgacaaga
     3121 ccgtgcgcaa ctacacttcg aatatcttcg ccaagcttca ggttgccaat cgagcggaag
     3181 ccattatcca ggcaagggat gctggcttgg ggcaagaggt ggatccaagg taaagccccg
     3241 atcgaaaata acaccagcac cggtttaata cgtggtgctg gcttgacttt agttactcac
     3301 cgtgggtagg gagcccaccc tctccgcagg taatttccgc caatccacgc caattttttc
     3361 gagacattgt ggagattggc gggtattttt ccaataaccc tgcaccaatg ttttcctttt
     3421 attgtcttta gttctattta ataactccgt cgtctaccag ataggctggc gattttgcca
     3481 tcggctgagt agggaccgag cagccacgcg cgtgcgccgt ttggcggtct gccttctgct
     3541 cccaactggc aagacgccaa aatttcccaa agattgagga cattgtctct agcttttggg
     3601 acttcttttg tccataatgg taacaatggt cccgttcttt ccatcaatcg cttccgtctt
     3661 tggaaact
//
LOCUS       DBNHOH010000262         9146 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_78901, whole genome shotgun sequence.
ACCESSION   DBNHOH010000262 DBNHOH010000000
VERSION     DBNHOH010000262.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9146)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9146)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9146
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_78901"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="AC16V7_04645"
     CDS             <1..976
                     /locus_tag="AC16V7_04645"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HIR6256441.1"
                     /translation="DLSRVDYSPELLRLGTLEREKLPDLMPPLTILGTLTQHAADDLG
                     LHTGVQVVIGTPDIPSAALGSGAVRDFEAHLYIGTSSWIACHVPFKKTDLFHNIASLP
                     SAIPGKYLVSDEQQTSGACLSFLRDNILFYHDELFTEADVPDVYNIFNQIAARAPAGS
                     GGVIFTPWLYGERTPSDDRYARGGFFNLSLQSTREHIIRAVLEGVAYNTRWLFGYVEK
                     FVGRSLDALNAVGGGASSDIWCQIMADVLGRDIHQMKDPILANARGAAYLASVAMGYM
                     TFDQISDQTQIKRTYAPNPENKLIYDAMFKEFLQIYQRTKPIYARLNR"
     gene            1047..2522
                     /locus_tag="AC16V7_04650"
     CDS             1047..2522
                     /locus_tag="AC16V7_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR6256442.1"
                     /translation="MNPRWVSVIEKMLRAIPSFNKAVHNEIETLTTDLYASLKPYRND
                     FRSYECLPPIGRDRDEILAEMQAIIAREESTWRNGFVSGGVYHGEDTHIKFLNQVYAL
                     QSQANPLHADIWPSISKFEAEIVSMTAHMMNAGTLGENNDVCGTVTSGGTESILLAMK
                     TYRDWARERKGITRPEMVLPVTAHAAFDKASHYFNIKPVLIPIDSDYKADINAARNAI
                     TRNTVVLVGSAPSFPHGIIDPIHELSELALQHGIGFHTDACLGGFLLPWVERLGYTIP
                     PFDFTLPGVTSISADTHKYGYAPKGTSVVLYRSTEMRHYQYYTASEWPGGMYISPTIA
                     GSRPGALSAACWAAMVTMGEEGYLEAARKILQTAVSIRQGIESIPDLYVLGDPLFVIA
                     FASSSLDIYQVTEHMHRRGWNLNRLHKPISVHLCVTLAHTQPGVAERFITDLKASVDE
                     ARLFPEKKTGMAPIYGMTASLPLRGVINDVLKRYIDALYKV"
     gene            2612..3553
                     /locus_tag="AC16V7_04655"
     CDS             2612..3553
                     /locus_tag="AC16V7_04655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6256443.1"
                     /translation="MILITGGTGFIGKALIRNLVESGNVVRTLIRPSARSPQLPHGVP
                     VEVAVSSLSDARSLRAAMVGVKAVYHLAGVERRGAYADLMAVDIQGTRLVAQAAADAG
                     VERIFFLSHLGADRASAYPVLKAKAIAEEYVRRSQVDYTILRSAIIFGPNDGFTTGLA
                     GLLSTIPYLFFYPGDGRTLIQPLWVEDLVTCMTWALEDDATRNQTYELGGPEYFTFLQ
                     VLEMVMQSLGLRRRLVSMRPPYLRAFTVALEALFPGLPVSVYWLDYLAANRTCALDTI
                     PRTFHLMPTRMSQRLDHLRGVNWRVSMLRNLLRRSPR"
     gene            3574..4488
                     /locus_tag="AC16V7_04660"
     CDS             3574..4488
                     /locus_tag="AC16V7_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256444.1"
                     /translation="MTIQLRILETPEEIVLVEELQRQVWPGSETEIVPAHVLLTAIHN
                     GGLVIGAFSDDREPGVQSLVGFVFGFPGLYFTPDGPHPKHCSHMLGVLPEYRDLGVGF
                     TLKRAQWQMVRHLGLDRITWTYDPLLSRNAYLNITKLGAVCNTYRRNEYGQLRDGINM
                     GLPTDRFQVDWWVNSQRVNHRLSRRARQPLDLAHYFAGGAEVINPSEIGTDGLPRPAA
                     MDHYEPLPRSADSGRPEVVPLLLVEIPADFMELKAQNFDLALQWRLHARNLFEDLFNR
                     GYLVTDFVFLKGSLPRSFYVLSFGESTL"
     gene            4485..5591
                     /gene="menC"
                     /locus_tag="AC16V7_04665"
     CDS             4485..5591
                     /gene="menC"
                     /locus_tag="AC16V7_04665"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HIR6256445.1"
                     /translation="MKIDRVALYHVRMPLVSPFETSFGRSETRDCVIIQVFADGLVGF
                     GECVADRDPGYSYETTGTAWHVLQEFLVPLLVGKSFSHPTGFQATFDQVRGHLMAKAG
                     LEMALWDLQGKAQGRSLRQLLGGVHPRVAVGVSVGIQPSPAALVQVVEEYVHQGYQRI
                     KIKIKPGHDVSETQAVRRALPHIRLQVDANSAYTLESAQVLLPLDELNLLLIEQPLFE
                     DDLWDHSRLQAQFTTPICLDESIISPRHARQALEMGACKVINIKAGRVGGLSQALAIH
                     DLCRSRGVPVWCGGMLETGVGRASNLALASLPGFSLPGDISASDRYYTQDITQERFKL
                     NPDSTIDVPDAPGLGVSIDQDALNKFTIQKDLLK"
     gene            complement(5647..7761)
                     /locus_tag="AC16V7_04670"
     CDS             complement(5647..7761)
                     /locus_tag="AC16V7_04670"
                     /inference="COORDINATES: protein motif:HMM:NF014628.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HIR6256446.1"
                     /translation="MADTAFRDHIRSALADENLQVALHANAVRRLNARQQAFSSLPRP
                     WEEMRQRAHAVRAVVIANLDELLAKFIERAEMNGMTVLRAADGTQAANTILEIARQNG
                     AQLVAKAKSMVSEEIGLNQILESAGLRVVETDLGEYIVQLRGEHPAHIITPAVHLRRE
                     QVGQTFYEKLGIPYTVDIPTLTLAARRVLRQVFLDAQIGLSGVNFGVVDSGTLCVVTN
                     EGNGRMVTSLPKVHIALMGIERLVPSLDDLALMLYLLPRSATGQKLSVYTNLIHGPAH
                     PDAPSGPQQRYLILLDNGRTNLRQSPLDEALYCIRCGACLNACPVFREIGGHAYVNPS
                     GKSSPYSGPIGSVISQGLFGQDFGNLARASSLCGACEEVCPVGIDLPKLLLRVRAGDV
                     QPLKPPPMEDKPGESDNKTPISNAPGWLAFGIRMYTWFATSASRFSLAQRLASFFGYL
                     ASPVSMWMRFPAITGWGYSKDFPRPALKPFRARWREMRENDYSASQHLLNSESSSLPP
                     NNGEQSSDQPAIDEQAQALAPLVQFSNEISAVGGEFVRCREEELSKHVLDLLQDRDIR
                     SILAWEEGYLPTGLLEELRAAGIAIHHEPDPEVRGGLTGASAAIAETGSLLVCEGGGR
                     PLTASLLPEVHIAVIQEHDIYENLTQVLNLQEVRQATSAVLITGPSRTADIEMTLTIG
                     VHGPKELIVVCMTPQEVAIHRE"
     gene            complement(7786..8523)
                     /locus_tag="AC16V7_04675"
     CDS             complement(7786..8523)
                     /locus_tag="AC16V7_04675"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015416196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR6256447.1"
                     /translation="MVAHQVQLFVTCIIDTLYPEVGEAVVRVLRRAGARVDFPSQQTC
                     CGQPAFNAGLRTQAREMAMHTMRVFEKTQGSIVLPSGSCTGMLRHGYLELFADDPQWL
                     GRARALSERTFEFSEYLVDVLGVVDLEARVAGKLTYHSSCHLLRDIGIDHQPKALLEK
                     VRGAELVELRETAECCGFGGVFSLEHPELSAAMLERKIANIETSGADWVVSCDGGCIT
                     HINGGLNRSGKPRRAIHIAQILDRSDL"
     gene            complement(8683..>9146)
                     /locus_tag="AC16V7_04680"
     CDS             complement(8683..>9146)
                     /locus_tag="AC16V7_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta/alpha barrel domain-containing protein"
                     /protein_id="HIR6256448.1"
                     /translation="TALPNFASRLVEAGASGLVLFNRFYQPDFDLELLEVVPNLVLSN
                     SHELRLPLRWIAILYGRVKTDFALTSGVHSAEDLIKSVMAGASVAMTASALLKNGVGR
                     AREILDGMVAWMEEREYESVEQMKGSMSQQAVAEPGLFERANYMKVLSSYY"
BASE COUNT         2084 a         2627 c         2324 g         2111 t
ORIGIN      
        1 ggatctctcc cgcgtggact actctccaga gttgttgcgc ctggggaccc tggagcgcga
       61 aaaactaccc gacctgatgc ctcctttgac cattctcggc acgctcaccc aacatgccgc
      121 cgacgacctg gggctgcaca caggtgtcca ggtggtgatc ggcacaccag acatcccgtc
      181 cgccgcctta ggatctggcg ctgtccggga ttttgaggcg cacctctaca ttggcacatc
      241 ctcctggata gcctgccacg tgcccttcaa aaagactgac ttattccaca atatcgcttc
      301 cctgccatct gctatcccag gaaagtatct ggtttcagat gagcaacaga catccggcgc
      361 ttgccttagc tttctgcgag ataacatcct cttttaccat gacgagctgt tcaccgaggc
      421 ggatgtgcca gatgtgtaca acattttcaa ccagattgcc gctcgcgctc cagccggtag
      481 cggtggcgtg atattcaccc cctggctata cggtgaacgc acacccagcg atgatcgcta
      541 cgcacgcggt gggtttttca acttgtcgtt gcaatctacc cgtgagcaca tcattcgcgc
      601 cgtgctagaa ggtgtggcat acaatacgcg ctggctattt ggctacgtgg aaaaattcgt
      661 tgggcgtagt ctggatgcgc taaacgcagt cggtggtggt gccagctcag atatctggtg
      721 ccagataatg gccgatgtat taggccggga tatacaccag atgaaggatc ctattctggc
      781 caacgcccgc ggcgcagcat acctggcctc ggttgccatg gggtatatga cctttgatca
      841 gatttctgac cagacacaga tcaaacgcac atatgctcca aatccagaga acaagctcat
      901 ctacgacgcc atgttcaagg agttcttgca aatctaccag cgtacgaagc caatttacgc
      961 tcgcctgaat cgatagcagc cagtaaacaa ggcaatagag gtgcaccttg aaatataaag
     1021 actcaattct tgccatcctc ggacgcatga acccacgctg ggtgtcagtg atcgaaaaaa
     1081 tgttgcgggc gataccttca ttcaataaag cagtgcacaa tgagatcgag acgctcacca
     1141 ccgatctata cgcttcactt aaaccctacc gtaatgattt cagaagttat gaatgtcttc
     1201 cacccattgg tcgtgaccgg gatgaaatcc tagcggagat gcaggcaatc atagctcgcg
     1261 aagaatctac atggagaaat ggtttcgtgt cggggggtgt ttatcacggc gaagacacac
     1321 acatcaaatt tctcaaccag gtttacgccc tgcaatccca ggccaatcca ttgcacgccg
     1381 acatatggcc tagcatcagc aagtttgagg ccgagatcgt ctctatgaca gctcatatga
     1441 tgaatgctgg aacgcttggt gagaataatg atgtttgcgg cacggtaact tccgggggta
     1501 cagagagcat cctgctcgct atgaaaacct atcgagattg ggcacgcgag cgcaagggta
     1561 tcacccgacc cgaaatggtg ttgcccgtta ccgcacatgc tgcctttgac aaagcatccc
     1621 attatttcaa catcaagcca gtcttgatac caattgattc cgattacaag gctgatatca
     1681 acgccgctcg taatgcgatc acgcgcaata cagtcgttct ggtaggctca gcaccatctt
     1741 ttccccacgg cattatcgat ccgatccacg agctatccga actagccctg caacacggta
     1801 ttggttttca caccgatgcc tgcctgggag gattcctgtt gccttgggtg gagagacttg
     1861 gttacaccat tccgcctttc gattttactc tgcccggcgt gacctccata tctgctgata
     1921 cgcataaata tggttacgct cctaaaggca cctctgttgt actgtaccgc agcactgaga
     1981 tgcgccatta ccaatactat accgcttccg aatggcctgg aggcatgtac atctccccga
     2041 ctattgccgg cagccgacca ggtgcactga gcgcggcgtg ctgggctgct atggtcacca
     2101 tgggtgaaga aggctatcta gaggctgccc gaaagatatt acaaaccgca gtttccatcc
     2161 gccaggggat tgagtcaatc cctgatcttt atgtgcttgg agatccgctg tttgtcattg
     2221 ctttcgcctc atctagcctc gatatttacc aagtgaccga gcacatgcac cgccggggct
     2281 ggaacctcaa caggctgcat aagccgatca gtgtgcatct atgcgttacc ctcgcgcaca
     2341 cgcagccagg tgtagccgag cgttttatta ctgacctcaa agcctctgtt gatgaggctc
     2401 gcctgttccc agaaaaaaag accggcatgg ccccgattta cggcatgact gcctccctgc
     2461 cgctgcgcgg cgtaatcaat gatgtattaa aaagatatat cgatgccctg tacaaggtct
     2521 aatcaaaatc tgtgatgatg gcgcttgggg gacgcgcacc tatcatggta gacttgatct
     2581 aaacacaaat aaggcataaa atcctcctgc gatgatatta attactggtg gaactgggtt
     2641 tatcggcaag gcgctcattc gcaaccttgt cgagagtgga aacgttgtgc gcacgctcat
     2701 ccgcccttcc gctcgctcgc cacaattacc ccacggcgtg ccagtggaag tcgcagtgag
     2761 tagcttgagc gatgcgcgca gcttgcgcgc ggcgatggta ggtgtaaaag ctgtctatca
     2821 cctggctggt gtggaacggc gtggagctta tgccgattta atggccgtgg atattcaggg
     2881 cactcgctta gtcgcccagg cagccgctga tgctggtgtg gaacgcatct ttttcctaag
     2941 ccaccttggt gcagatcgcg cctccgccta tccagtactc aaagccaaag ccatcgctga
     3001 ggagtacgta cgccgcagcc aggtagatta caccattttg cgctccgcca taatatttgg
     3061 accgaatgac ggtttcacga ctggccttgc cgggctgctc agcaccatcc cttatctttt
     3121 cttctacccc ggtgatggtc gcacgctcat ccagcctttg tgggtcgagg acctggtcac
     3181 ctgcatgacc tgggcactgg aagacgatgc tacccgaaac cagacttacg agctcggcgg
     3241 gcctgagtat ttcacctttc tgcaggtcct tgagatggtc atgcaatcgc ttgggctgag
     3301 acgccgtttg gtgtctatgc gccctcctta cctgcgagca ttcaccgtag cactcgaagc
     3361 ccttttcccc ggccttccgg tatctgtcta ttggttggat tatttggcgg caaaccgcac
     3421 ctgtgccctg gatacgatcc cgcgcacctt tcaccttatg ccaacccgca tgtcgcaacg
     3481 cctggatcat ctgcgggggg tgaattggcg ggtctccatg ctgcgaaacc tgttgagaag
     3541 atctcctcga tgagtagagg tggtttgact aagatgacaa ttcaattgcg gattttagaa
     3601 acgccagaag agatcgtctt ggtcgaggaa ctccagcgcc aggtttggcc gggcagtgaa
     3661 acagagatcg ttcccgccca tgtcctgctg actgccatcc acaatggcgg gttggtgatt
     3721 ggagcctttt ccgacgatcg tgagcctgga gttcaatcgc tggtcggatt tgtttttggc
     3781 ttccccggtc tctactttac ccctgatggg ccgcatccca agcactgttc ccatatgttg
     3841 ggtgtcctgc ctgaatatcg cgacctaggg gtaggattca ccctcaagcg cgcccaatgg
     3901 caaatggtgc gccaccttgg tttggaccgt atcacatgga cctacgatcc tctactcagc
     3961 cgcaatgcct atttaaacat aaccaagctg ggcgcagttt gcaacacata ccggcgcaac
     4021 gaatatggtc aattgcgcga cgggattaat atgggcttac ccactgaccg attccaagtg
     4081 gattggtggg tcaactcaca acgcgtcaac caccgcctca gccggcgcgc tcgccaacct
     4141 ctcgacctgg ctcattattt tgctggcggc gcggaagtga ttaacccttc agagatcggc
     4201 accgacgggc taccccgacc ggctgcaatg gatcattatg aacctctccc ccgctctgcc
     4261 gattctggca ggccagaagt agtccccctc ttgctcgtcg aaatccccgc ggatttcatg
     4321 gaactcaaag cccagaattt cgacctggca ttgcagtggc gtttgcatgc ccgcaacctg
     4381 tttgaagatt tattcaaccg cggatacttg gttacagatt ttgtctttct aaaaggtagc
     4441 cttccgcgca gtttctacgt tttgagtttt ggagagagca cactatgaaa atagacaggg
     4501 ttgccctgta ccacgttcgc atgccgcttg tatccccttt tgagacctcc ttcgggcgca
     4561 gcgaaacacg cgattgtgtc atcatccagg tatttgcaga cggtctggta ggctttgggg
     4621 agtgcgttgc tgaccgggat cctggctact cctatgaaac caccgggaca gcgtggcacg
     4681 tccttcaaga atttctcgta ccactactgg ttggaaaatc cttctcccat cctacaggtt
     4741 tccaagcaac ttttgaccag gtgcgaggtc acctgatggc aaaggctggg ctggagatgg
     4801 cactctggga tctgcagggt aaagctcagg ggcgttctct acgccagcta ctgggtggag
     4861 tgcaccctcg cgtggcagtg ggcgtctcgg ttggcatcca gccctcaccc gcagccctgg
     4921 ttcaggtagt ggaagaatat gtccaccagg gttaccaacg gatcaaaatc aagatcaagc
     4981 ccggtcatga tgtgagtgag actcaggctg tgcgccgtgc gcttccgcat atcaggctgc
     5041 aggtagacgc caactcagcc tacactctcg aaagcgccca agttttgctg cccttggacg
     5101 agctaaactt gctgctgatc gagcagccgc ttttcgaaga cgatctctgg gatcacagtc
     5161 gccttcaggc gcaattcact acccccattt gtctggacga gagcatcata agcccaaggc
     5221 acgcccgcca ggccctggag atgggtgcct gtaaggtgat taatatcaaa gccggacgcg
     5281 tgggcgggtt gagccaagca cttgccatac acgatctttg tcgctcacgt ggcgtgccag
     5341 tctggtgtgg cggtatgcta gaaaccggtg tggggcgcgc ttccaacttg gctctggcat
     5401 ccttacccgg cttctctcta ccaggagata tctctgcctc agatcgttac tacacgcagg
     5461 atattacgca agagcgcttc aaacttaacc cagatagcac catcgacgtg cccgatgccc
     5521 ctggtttggg tgtgagcatt gatcaggatg ccctgaataa attcacaatt caaaaagatc
     5581 ttctcaagtg atcttagaca ggaatctcca tagtttcaca cagcgcccct gacgccgcac
     5641 tttcccctac tctctatgga tggcgacttc ttgaggggtc atacatacta cgatcagctc
     5701 tttgggacca tggaccccaa tggttagtgt catttcaata tctgcggtgc gcgaaggtcc
     5761 ggtgatcaac accgccgaag ttgcctgtct gacttcttgc agatttaaaa cctgcgtgag
     5821 attttcatag atatcgtgct cttggatcac cgcgatgtgt acctcgggca acagcgaggc
     5881 agtcaatggg cgccctccac cctcgcaaac cagaagagac cctgtctctg caatcgccgc
     5941 gctagcacct gtcagcccgc cgcgcacctc gggatcaggt tcatgatgga tggcaatccc
     6001 ggctgcgcgc aactcttcta aaaggccagt cggcagataa ccctcctccc aggccagaat
     6061 ggagcggata tcccgatctt gcaacagatc aagcacgtgc tttgaaagct cctcttcgcg
     6121 acaacggacg aattctcctc caactgcact gatttcattc gaaaattgca ccagcggcgc
     6181 caaggcttgt gcctgctcat caatggctgg ttgatcgctg gactgctcac cattgtttgg
     6241 cggaaggcta ctcgattcgc tattcagcaa atgctgactg gctgagtaat cattctcacg
     6301 catctcacgc cacctggctc gaaaaggctt cagcgcagga cgtgggaaat cctttgaata
     6361 gccccaaccg gtgatcgccg ggaaacgcat ccacatggat acaggcgaag ccaaatagcc
     6421 aaaaaaactt gccaaacgct gtgccaggga gaaacggcta gccgaagtcg cgaaccaggt
     6481 atacatgcgg ataccaaatg ccaaccagcc gggtgcgttt gagatgggcg ttttgttatc
     6541 ggactcaccc ggcttatctt ccataggggg tggttttaat ggctgcacat ctccggcgcg
     6601 cacgcgcagc aacaatttcg gcaggtcgat acctaccggg caaacctcct cacaagcccc
     6661 gcataggctg gaggcgcgcg ccagattccc aaaatcctgt ccaaaaagac cttgtgagat
     6721 aaccgagcca atcggtccag aatatgggct gctcttacca gatggattta cataggcgtg
     6781 accaccgatc tcgcgaaata ccgggcaggc attcaggcat gcaccgcaac ggatgcaata
     6841 aagggcttca tccaggggcg attggcgcag gttggtgcgc ccgttatcca gtaggatcag
     6901 gtatcgctgt tgcggcccgc tcggtgcatc cgggtgtgct ggcccatgga tcagattggt
     6961 gtacacgctg agcttctgcc ccgtggctga gcgcggcaac aggtagagca tcagggcaag
     7021 atcgtccaga ctgggcacca ggcgctcaat gcccatgagc gcaatgtgta ctttaggcaa
     7081 gctggtgacc atacgcccgt tgccttcgtt ggtgacaaca cacagggtgc cgctgtccac
     7141 cacaccaaaa ttgacccctg aaaggccaat ctgtgcatcg agaaacacct gtcggaggac
     7201 ccgtcgcgct gccagggtca gcgtggggat gtctacggtg tatggtattc cgagtttttc
     7261 gtagaaggtt tgcccaactt gttcccgccg taagtgtaca gccggggtga tgatgtgcgc
     7321 cgggtgctca ccgcgcagct gcacgatgta ctcgcccagg tcggtttcaa ccacgcgcaa
     7381 gcccgccgat tccaagatct ggttcaaccc gatctcctcg gaaaccatgc tcttggcttt
     7441 ggcgaccagt tgcgccccat tttggcgggc aatctccaaa atagtgttcg ccgcctgcgt
     7501 cccatcagcg gctcgcagca cggtcatacc attcatctcg gcgcgctcaa tgaatttcgc
     7561 taacaactca tccagatttg ctatcaccac cgcccgaaca gcatgagctc gctgcctcat
     7621 ctcctcccag ggtcttggca agctggaaaa cgcctgctgg cgggcattca gacggcgcac
     7681 agcgttggcg tgcagggcta cttgcagatt ctcatccgcc agtgctgagc gaatatggtc
     7741 acgaaatgct gtgtctgcca caagtattgc cttgatggtc aaatatcata gatcacttct
     7801 atccaatatc tgggcgatat ggatagcccg cctcggcttc ccagacctgt ttaacccccc
     7861 gttgatgtgg gtgatgcacc ccccgtcaca ggaaactacc caatctgcgc cgctggtctc
     7921 aatattagcg attttccgct ctaacatcgc ggcagataat tcaggatgct ctaaagagaa
     7981 aactccgcca aatccgcagc actccgcagt ttcacgcaat tcaactagct ccgcgccacg
     8041 cactttctcg agcagagcct tgggttggtg gtctatgccg atatcgcgca acaggtggca
     8101 agacgagtgg taggttagct tacccgccac gcgcgcctct agatcgacga cccccagcac
     8161 atctaccaga tattcgctga actcgaaggt gcgctcgctg agagcgcggg cgcgccccaa
     8221 ccattgcggg tcgtcggcga acaattccag gtaaccatgc cggagcatgc ctgtacacga
     8281 gccagacggg agaacgatcg agccctgagt tttctcaaag acccgcatcg tatgcattgc
     8341 catttctcgc gcctgggtgc gtaggcctgc gttgaaggcc ggctgcccac agcaagtctg
     8401 ctgggatgga aagtccacgc gggcaccagc ccgcctcaac acccgcacta ccgcctcacc
     8461 cacctctgga tatagcgtat caataataca agtgacaaaa agttgaactt ggtgagcaac
     8521 catattcctg tagtataagc caataggtcg aaaaaagtag ccgaactggg aagtataaaa
     8581 acccatttcg gctacgttgt gctaaccctt ttgcaccggg ggattaatga taaccacctg
     8641 ccctcatatt tcaaatgaaa ccaggattta gtcagcaggg cattaataat atgaactgag
     8701 caccttcata tagtttgccc gttcaaaaag acctggttca gcaactgcct gctggctcat
     8761 gctgcctttc atctgctcga ccgactcgta ttcacgctcc tccatccagg caaccatgcc
     8821 gtccaggatt tccctggcac gacccacacc gttcttgagt aacgccgatg cggtcatcgc
     8881 cacactggca ccagccataa ccgacttgat caaatcttca gcactatgca ctccactggt
     8941 gagggcaaaa tcagtcttaa cccgtccgta aaggatagca atccaccgta agggcaagcg
     9001 caattcatgc gagttgctca acaccaggtt tggtaccacc tctaacaact ccaggtcaaa
     9061 atctggttgg tagaaacggt tgaataacac caacccgctt gccccagcct ccaccagccg
     9121 gctggcgaag ttcggcaaag cagtgt
//
LOCUS       DBNHOH010000263         2072 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_655856, whole genome shotgun sequence.
ACCESSION   DBNHOH010000263 DBNHOH010000000
VERSION     DBNHOH010000263.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_655856"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="AC16V7_04685"
     CDS             <1..682
                     /locus_tag="AC16V7_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HIR6256449.1"
                     /translation="PNGAGKSTLFKVIAGVLQHTSGEVNIYGHEPGGHICIAYVPQRS
                     QVDWKFPVSVADVVMMGRISKLGLLRWPKPRDWDYVRQSLQVVGLDELADRQISELSG
                     GQQQRMFIARALAQEAELMLMDEPFTGLDINSQEDILSIMDELRQRGVTVLVSTHDLN
                     LAGEHFDRVLLLKRRVYGLGLPSEVFVPDLLLSAFGGHLRLLQVETEVVALGDTCCDD
                     EASHEHLA"
     gene            666..1532
                     /locus_tag="AC16V7_04690"
     CDS             666..1532
                     /locus_tag="AC16V7_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIR6256450.1"
                     /translation="MNTWLDLLLEPFAYAFMVRGMLAALLVGIVCATVGTYVVLRGMA
                     FFGDALAHAILPGVAVGYLLGGGAREPLFWWSLLTAIAASIGIGAISKGAQLREDTAI
                     GIVFAGMFALGIALISTVRNYTNDLAHFLFGDVLGVSASDLVRIAIFGLIILLTVLLF
                     YKEFLVLSFDPLLAATLRLPARVLEYILLILIALAVVVSLQTVGVALMVAMLVTPAAT
                     AYLLTRRLPYMMALGAIIASLSGVIGLYLSYYVSVSSGAAIVLTSTLIFVIVWGARTL
                     RRAYRGRSTSQA"
     gene            complement(1583..>2072)
                     /locus_tag="AC16V7_04695"
     CDS             complement(1583..>2072)
                     /locus_tag="AC16V7_04695"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6256451.1"
                     /translation="FGLKIAALGAASAVIGLSELGGESLTAILVDRFGKLRAVGIGLM
                     VNCLAAIALPILGDSIPGALIGLFCFYISFEFTVVSSIPLMSEIVPSARATLMSSNAG
                     ALSLGRALGALLASPLYALGFWWSCLAAVLLNLLAMLALLKLRKEYGDGKEIGKLSTA
                     KP"
BASE COUNT          392 a          554 c          614 g          512 t
ORIGIN      
        1 gcctaatggc gcgggtaaga gcacattgtt taaagtgata gctggcgttc tccagcacac
       61 cagtggcgag gtgaatatct acgggcatga gccagggggg catatttgta ttgcctatgt
      121 accgcaacgc agccaagttg attggaaatt cccggtgagc gttgccgatg tggtgatgat
      181 gggccgcatc agtaagctgg gtttgctgcg ctggccaaaa cctcgcgatt gggactatgt
      241 gcgccaatca ctgcaggtgg tgggcttaga tgagctagca gaccggcaga tctcggagct
      301 gtccggcggg caacagcagc ggatgttcat cgcccgtgcg ctggctcagg aagccgagct
      361 gatgcttatg gatgagccct tcaccggctt ggacataaat tcccaagagg atatcctgag
      421 tatcatggac gagctgcgcc agcgaggtgt gaccgtgctg gtctcgaccc atgatctcaa
      481 tctggcggga gagcatttcg accgtgtatt actgctcaaa cggcgggttt atgggcttgg
      541 cttgccttcg gaagtttttg tccccgatct cctgttgagc gcttttggtg ggcatctgcg
      601 cctgctgcaa gtcgagaccg aggtagtggc tctcggagac acctgctgcg acgacgaagc
      661 cagtcatgaa cacctggctt gacctgctcc tcgagccgtt tgcgtatgcc ttcatggtgc
      721 gcggcatgtt ggctgcgctg ctggtgggta tagtctgcgc caccgtgggg acctatgtag
      781 tgctgcgcgg catggctttc ttcggtgatg ctctggcgca cgccatcctg ccgggtgtgg
      841 cggtaggcta ccttctcgga ggaggggcgc gcgagccgct cttctggtgg agcttgctga
      901 ccgctattgc ggcctccatc ggcatcggcg ctatcagcaa gggggcacaa ttgcgcgaag
      961 atacggcgat tggtattgtc tttgccggca tgttcgcctt ggggatcgcc ctgatttcca
     1021 ctgtgcgcaa ttacaccaat gatctggctc acttcctttt tggcgatgtt ctgggtgtat
     1081 ccgcctcgga tctagtccga atcgccatct tcggcttaat tatcctgttg acggtgttgt
     1141 tgttttataa agagttcctg gtgctctcat tcgaccccct gcttgccgcg actttgcgcc
     1201 tgccagcgcg cgttttagaa tatattctct tgatattgat cgcattggca gtagtggtct
     1261 cgcttcagac ggttggagtg gccttgatgg tcgccatgct ggtgacccct gccgcaacgg
     1321 catatctgtt gacccgccgc ctaccttata tgatggcctt gggcgcgatc attgcttcgc
     1381 tgtctggtgt gatcggcttg tacctgtcgt attatgtcag tgtatcatct ggtgctgcca
     1441 tcgtgctgac cagcacgctc atttttgtca ttgtgtgggg ggcgcgcact ctgcggagag
     1501 cttacagggg taggtctact tcccaagctt aacttgtatc tagaatcaaa ttcttctgcc
     1561 aattttatcc atgcgcacac ttttacggct tagcggtgga taatttccct atctctttac
     1621 catccccgta ttcttttctc aacttgagaa gggccagcat agcaagcagg ttgagcagaa
     1681 ccgctgccag gcagctccac caaaatccaa gcgcatacag cggagacgcc agcagcgcgc
     1741 ccagggcacg gcctaatgag agagcccccg cgttggagga catgagtgtg gcgcgagcgg
     1801 agggcacgat ctcgctcatt aaagggatgc tgctgacgac ggtgaattcg aaggaaatat
     1861 agaagcaaaa caagccgatc agggcgccgg gtatgctatc ccctaggatg ggcaacgcga
     1921 tggctgccag gcagttgacc atcaagccaa tgcccaccgc gcgcagcttt ccaaatcggt
     1981 ccaccagaat ggcggtcaga ctttcgccgc ccaactcgga gaggccgatc actgccgagg
     2041 ctgcgcccag ggcagcgatc ttcagtccga at
//
LOCUS       DBNHOH010000264         3897 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1617281, whole genome shotgun sequence.
ACCESSION   DBNHOH010000264 DBNHOH010000000
VERSION     DBNHOH010000264.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1617281"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="AC16V7_04700"
     CDS             complement(<1..481)
                     /locus_tag="AC16V7_04700"
                     /inference="COORDINATES: protein motif:HMM:NF014750.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6256452.1"
                     /translation="MQGWTGKILDINLTDGSIETIPLDMEMARLFLGGRGLGARLLWD
                     LVGPEVSPLSPENVLIFTTGPITATGSQTSNRFNVSTKSPLTDTILHANSGGWWGMQF
                     KRTGYDVLIVRGKAQSPVMIEITPEGVELKKADHLWGKGVFETTEALGQSRNQRNVLC
                     "
     gene            complement(599..904)
                     /locus_tag="AC16V7_04705"
     CDS             complement(599..904)
                     /locus_tag="AC16V7_04705"
                     /inference="COORDINATES: protein motif:HMM:NF041918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HIR6256453.1"
                     /translation="MKIKLFGGLRQKAGSKEQTASGATIREALEGICLDNETLRAAIF
                     DGNVLHPHVRVMVNGRDCELAQGLDTPVSDDDQIAVFPPIAGGQCTRRGGTEKMRSG"
     gene            complement(958..2415)
                     /locus_tag="AC16V7_04710"
     CDS             complement(958..2415)
                     /locus_tag="AC16V7_04710"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HIR6256454.1"
                     /translation="MPAITVLAIDIGAESGRVMAVHFDGKQLASEELHRFPNTTITVN
                     CTLHWDFLRLWGDIQEGIEKGKALNPASIGLDTWGVDFGLLDSQGNLIGNPVHYRDGR
                     TAHMMDIAFARVPQADIFAQTGIQFMPINSLYQLLSLVESQSPQLQLADTFLTAPDLL
                     NYWLTGVKVCEFSNATTTQLFNPTTGTWATGIMAKLGIPSHIFPEIVPPGTRLGTYKG
                     IPVIAPACHDTGSAVAAIPTQRHDFAYISSGTWSLVGLEVNSPILTPEALTANVTNEG
                     GVYGTYRLLKNVMGLWILQQCRATWAAEGEVYTYAELVNNAAQAEPLATVFDPNDPAF
                     LSPGDHPQLIRDLCSKTNQPVPETPGAVVRSVLESLALAYREVLELVSAVANRHVSVL
                     YIVGGGAQNQLLNQMTADATRLPVIAGPIEATVIGNALVQLITLGEITDISQARQILA
                     NMAELKQYEPVGNAGSKTSVWEDAYDRYRRNRPPR"
     gene            complement(2418..3692)
                     /locus_tag="AC16V7_04715"
     CDS             complement(2418..3692)
                     /locus_tag="AC16V7_04715"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019318 - hexose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HIR6256455.1"
                     /translation="MNQQPTTTQIEQAYKQARERYAAVGVNTEEAMARLADIAISLHC
                     WQGDDVVGFEDPDRGLSGGIMTTGNYPGKARTPNELRQDLDKVYSLIPGTHRLNLHAI
                     YLETDNKVPRNEIEPKHFARWVDWAKENNHGIDFNPTCFSHPLADDGFTLSHPDVGIR
                     QFWIEHCIASRVIGAHFGRELGTPAITNIWIPDGYKDTPVDRLGPRRRLRDSLDRILT
                     EPTDRRYNLDSVESKLFGIGSESYVVGSHEFYLGYATANQTLLCLDAGHFHPTESIAD
                     KISAVLLYVPELLLHISRGVRWDSDHVVTLTDELQAMMGEIVRGNYLDRVHIGLDFFD
                     ASINRIAAWAIGARNALRALLMALLEPTEQMRALEMAGDFTARLALFEELKGMPLGAI
                     WDSYCLQQNVPVGMTFLDEIRAYENKVLAQRG"
BASE COUNT          857 a         1074 c         1004 g          962 t
ORIGIN      
        1 tgcacaacac attgcgctgg ttgcggcttt gtcccaatgc ctcggtggtt tcaaacacac
       61 ccttgcccca caggtgatcc gcttttttta actccacgcc ttccggtgtg atctcgatca
      121 tgaccggcga ttgcgctttg ccacgcacaa tcagcacgtc gtagccggtg cgcttgaact
      181 gcatccccca ccagccgcca ctgttggcat gcagaatagt gtcggtcagt gggcttttgg
      241 tgctaacatt gaagcgatta ctcgtctgtg aaccggttgc tgtaatggga cccgttgtaa
      301 agatgagcac gttttcgggc gagagtggac tcacttctgg tccgaccaaa tcccacaata
      361 accgtgcacc taggcctcgc ccccctaaaa agaggcgtgc catttccata tctaacggta
      421 ttgtttcaat gctgccgtct gtgaggttaa tatccagtat ttttccagtc caaccttgca
      481 taagtacctc ccaacaaatt agatttatat tcgccgacga tgagctaccg ctagtcaaaa
      541 actcctacga gagttacacg aaaagataag aataacaaaa gagagatatt ctctattttt
      601 atccagatcg cattttttcg gttcctccgc gtctagtgca ttgaccacct gcaatcggtg
      661 ggaagacggc gatctggtcg tcatcagata cgggcgtatc caaaccttgc gccaattcgc
      721 aatcgcggcc gttcaccatc acacgcacgt ggggatgcag cacattgcca tcaaatatcg
      781 ctgcacgtaa cgtttcatta tctaaacaaa tgccttccag cgcttcccga atcgtcgcgc
      841 ctgatgccgt ttgctctttg ctcccggcct tctggcgcaa cccgccgaac agcttgattt
      901 tcatcctaat ccctcttact caacgataat gtcgctccga aattgtgaat ctcctcgtca
      961 tcgaggcgga cgatttcgcc gataacggtc atatgcgtct tcccaaacgg atgtcttaga
     1021 gcctgcgttt cctactggct cgtattgctt cagttcagcc atattggcta atatctggcg
     1081 tgcctggctg atgtctgtaa tttcacctag ggtgatgagt tgcaccaagg cgttgccgat
     1141 gacggtggcc tcgataggtc cagcgataac gggcaggcgc gtggcatccg cggtcatctg
     1201 gttgagcagc tggttttgcg cgcctccgcc cacgatgtat agaactgaga cgtggcggtt
     1261 ggcaacggca cttaccagtt ccagcacctc gcgatacgca agcgcaagac tttccaggac
     1321 gctgcgcacc accgctcccg gcgtttctgg aacgggctgg tttgttttgc tgcacaagtc
     1381 acgaatgagc tgcgggtggt cgccagggga gagaaatgca ggatcattgg ggtcgaaaac
     1441 ggttgctagt ggctccgcct gtgctgcatt gttcaccaat tcggcatagg tgtacacctc
     1501 gccttcagcc gcccaggtgg cgcggcactg ctgtaaaatc cacagcccca tcacgttttt
     1561 cagcaaccgg taagtcccgt aaacgccgcc ttcgttggtg acattagctg tcagcgcttc
     1621 cggcgtcagt atcgggctat tcacttccag gcctaccagg ctccaggtac cgctgctgat
     1681 gtaagcgaag tcatgtctct gggtaggaat ggccgccacg gcactgcctg tatcgtgaca
     1741 ggccggggca atgaccggga tacctttata ggttcccaat cgggttcccg gtgggacgat
     1801 ttcggggaaa atgtggctgg ggatgcccaa cttcgccatt atcccagtag cccaggtgcc
     1861 ggtggtaggg ttgaacagct gggtggtggt ggcattgcta aattcacaca ccttaacgcc
     1921 ggtcagccaa tagttgagca aatcaggcgc tgtcaaaaag gtgtcggcaa gttgcaactg
     1981 cggcgattgg ctttctacca ggctcagcaa ctggtagagg ctattgatgg gcataaactg
     2041 gataccggtc tgggcgaaga tgtcagcttg gggaactctg gcaaaagcaa tgtccatcat
     2101 gtgcgcggtg cgcccgtcgc gatagtgcac cggattgcca atcaggttgc cctggctgtc
     2161 cagcagacca aaatccacac cccaggtgtc caggccaatg ctggcaggat tgagcgcttt
     2221 gcccttctct attccttctt gaatatctcc ccacaggcgt aagaaatccc aatggagtgt
     2281 gcagttgacg gtaattgttg tgttgggaaa acgatgcagt tcttccgacg ccagttgctt
     2341 gccgtcgaaa tgcaccgcca ttactcggcc tgattcggct cctatatcga tggctaggac
     2401 tgttatggca ggcaatatta acctcgttgc gccaacacct tattttcgta ggcgcgaatt
     2461 tcatccagaa aggtcatgcc aaccggcacg ttttgctgca aacaatagct atcccaaatt
     2521 gcacccaacg gcatcccttt caactcttcg aatagagcca gccgggctgt gaaatctcca
     2581 gccatctcaa gcgcacgcat ctgctcggtg ggctccaaca aggccataag cagcgcccgc
     2641 agtgcgttgc gcgccccgat agcccaggcg gcaatgcggt tgatgctggc atcgaagaaa
     2701 tccaggccaa tatgtacacg atccagatag ttgccacgca caatttcacc catcatcgcc
     2761 tgcagctcat ccgtcaaggt gaccacgtgg tcgctatccc agcgcacgcc gcggctgatg
     2821 tgcagcagca gttccggaac gtacagcagc accgctgaaa ttttgtcggc gatggattct
     2881 gtggggtgaa aatgaccggc atcgagacaa agcaatgtct ggttggctgt ggcgtagccc
     2941 aaataaaatt cgtgagagcc gacaacgtag ctttcagagc cgataccgaa taatttactt
     3001 tccaccgaat ccaggttgta gcgccggtcc gttggttcgg tgaggattct gtccagcgaa
     3061 tcacgcaagc gccggcgtgg tcccagccga tccaccgggg tatctttata gccgtctggg
     3121 atccagatat tggtgatggc aggcgtgccc agttctcgcc cgaaatgcgc tccaatcacc
     3181 cggctggcaa tacaatgctc aatccaaaat tgacggatac caacatcagg atgtgataac
     3241 gtgaagccat catcagccaa cgggtgcgaa aaacaagttg ggttgaaatc tattccgtga
     3301 ttgttctcct ttgcccaatc cacccacctg gcgaaatgtt ttggttcaat ctcgttacgc
     3361 ggcaccttat tgtctgtttc caggtagatt gcatgcaagt tgaggcgatg agtgccggga
     3421 atgaggctgt agactttatc taagtcctgg cgcagttcat tgggcgtgcg tgctttaccc
     3481 ggataattgc ccgttgtcat aataccaccg ctcaaacctc gatccgggtc ttcaaaaccg
     3541 accacgtcgt caccctgcca gcagtgcagg ctgatagcga tatcggcaag ccgtgccatt
     3601 gcttcttccg tgttcacacc gacagccgca tatcgctctc tggcctgttt gtacgcctgc
     3661 tcaatctggg ttgtggttgg ttgttggttc atgactgctc ccgtgtactt gtggggtgat
     3721 tattccggaa tcaccctaaa aagctttcat tttctgtaat gtgttgcagc actggtcgcc
     3781 tcctctaatc caggtgaaaa tactcttcca gctcaaggaa agtctcatca ggacgagcgt
     3841 tggcatcgat gaaatgggac atgaatttct gccagcgcgt gtttacctct ttggcgg
//
LOCUS       DBNHOH010000265         1509 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1104754, whole genome shotgun sequence.
ACCESSION   DBNHOH010000265 DBNHOH010000000
VERSION     DBNHOH010000265.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1104754"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1359)
                     /locus_tag="AC16V7_04720"
     CDS             complement(<1..1359)
                     /locus_tag="AC16V7_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256456.1"
                     /translation="MRTEGLKPGFPWLPLTILNPREYSDHHLLFHIALIPFTLGDLRE
                     GAKLAAVLFPAIAFLCIWWLLRVQRVPYSALWALGLLAISEAFIFRMSLVRRQSLTLA
                     VMAVALHWMLTKKYVRLIPLGFVYVWLYDAFPLLLTLTTIYVASILLIERRLELRPLI
                     YCGVGVTLGLVLNPYFPNDIIFAIRHLAPKITETTALDVGNEWYPYQTSQLLENSPLT
                     LVAFFSGVLALGLRERRMETRTATSLFIALLFGLLLLQSRRFIEYFPPFALIFAAFAW
                     SPLLQLQDTNQDDINVSDPSLRFLKYSILGLRWRQVTAVVVLVALLIPGIWLTVRDAQ
                     KSVKSSKPYTLFSGASEWLKANTPSGSRVFQTDWDDFPRLFYHNTHNTYLIGLDPTYM
                     QLYNADLYDLWEKITEGDVERPSQFIYDRFGANFVVSDLNHKDFIRRAEEDPGLEEVY
                     RDE"
BASE COUNT          422 a          330 c          406 g          351 t
ORIGIN      
        1 ctcatcgcgg tatacctctt ccagaccggg gtcctcttcg gctctgcgga tgaagtcttt
       61 atggtttaaa tcgctcacca cgaaattggc gccaaagcga tcgtaaataa attgggaagg
      121 tcgttcgaca tctccttcgg tgatcttttc ccagagatca tacaaatcgg cgttgtacaa
      181 ttgcatatag gtgggatcta ggccgatcaa ataggtattg tgcgtgttat ggtagaagag
      241 acgaggaaaa tcatcccaat ctgtctgaaa tacacgcgag ccagatggtg tgttggcttt
      301 caaccattcg gatgccccag aaaaaagtgt gtatggttta gaagatttta cgctcttttg
      361 cgcgtcgcgc acggtcagcc atatcccagg gattagtaag gcgaccagta caacgactgc
      421 ggtcacctgg cgccagcgta atcctaaaat ggaatatttc agaaagcgga ggcttggatc
      481 ggatacatta atgtcatcct ggttggtgtc ttggagttgc aagaggggac tccaagcaaa
      541 tgctgcgaat atcaaggcga atggagggaa atattcaata aatcgccgcg attgaaggag
      601 caataaacca aaaaggaggg cgatgaagag gctcgttgca gtgcgtgttt ccatgcgtcg
      661 ttctcgcaag cccaaagcaa gcaccccgga aaagaaagcg accagtgtca gtggggaatt
      721 ctccagcaac tggctggttt gatagggata ccattcgttg ccgacatcta aggcagtcgt
      781 ctccgtgatt ttgggtgcga ggtgacgaat ggcgaatatg atgtcgtttg gaaaatatgg
      841 attaagaacc aaccccaggg ttacccctac tccacaatag atcagggggc gcagctccaa
      901 gcggcgttcg atgagtagga tcgaagcgac gtaaatggtg gttagtgtta ataaaagggg
      961 aaatgcatcg tatagccaga cgtacacgaa tcccagggga atcaaacgaa cgtatttttt
     1021 cgtcagcatc cagtgtagag cgactgccat gacagccaag gtgagtgatt gtctccttac
     1081 caggctcatg cgaaaaataa aagcctccga aattgctagc aatcccagcg cccacagcgc
     1141 gctgtagggg acacgctgca ctcgcagcaa ccaccaaatg cacagaaaag ctatggcagg
     1201 gaagagcaca gcagctaatt tggctccttc tcgtaagtca cccaatgtga agggtattaa
     1261 tgcaatgtga aagagaagat ggtggtcgct atattcgcgc gggttaagaa ttgtaagcgg
     1321 tagccaaggg aaaccgggtt ttagtccctc cgttcgcatg atgctcgcaa agcgtatgtg
     1381 gaaatagcca tctactcctg cgagattgtc tgtggagaat tgcactatgc ttaggagggc
     1441 gatgaaaatt agcatcatcc caattacaga gagaaaagtt ctcaccttag ggtgatccag
     1501 cgtgattct
//
LOCUS       DBNHOH010000266         5301 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1105004, whole genome shotgun sequence.
ACCESSION   DBNHOH010000266 DBNHOH010000000
VERSION     DBNHOH010000266.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1105004"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="AC16V7_04725"
     CDS             complement(<1..121)
                     /locus_tag="AC16V7_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008408126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HIR6256457.1"
                     /translation="MTPLQIVFILVAAATLGSALLVVTTRNLVHAALWLVASLF"
     gene            complement(137..1372)
                     /gene="nuoH"
                     /locus_tag="AC16V7_04730"
     CDS             complement(137..1372)
                     /gene="nuoH"
                     /locus_tag="AC16V7_04730"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HIR6256458.1"
                     /translation="MGDPVTFIASWLKGLLLGWGLAPGFVTLILAVIGVVVMATVALV
                     LDIFLVWVERKVVARFQDRLGPNRLGPFGLVQPVADIIKLLIKEDITPIGADRIVYNL
                     APIMALGTVLTLWAVLPISATIYGSNINVGVLYIVAIGAISTLGIIMAGWASNNKYAL
                     LGAFRTVAQMVSYEVPMVITLLVPVILARSMGLKDIVDGQTVWFLFMAPLAALIFLVS
                     NIAELGRAPFDLTEAESEIVAGFHIEYTGMKFGLFYAGELLHALTVGAIFSTLFLGGW
                     RGFGAETYPIIGVFWFFLKAFFMYWVIMWVKYTFPRIRIDHMLNFNWKFLTPLSLVVL
                     IVTAIADKLVPRDNLLVYTLWMLLANIVIAWFTLQILRARARVERKTVGEPRPVATPP
                     QQPNVMPSSASQPPAGTAQ"
     gene            complement(1457..1813)
                     /locus_tag="AC16V7_04735"
     CDS             complement(1457..1813)
                     /locus_tag="AC16V7_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HIR6256459.1"
                     /translation="MLNDWLFIGVFLLLAPIFPGLALVVPRLIAPRKPNPIKLQTYEC
                     GIETVGDSWVQFRVQYYIFALVFLIFDVETVFLYPWAVAFDQLPLFAVLEGVLFILIL
                     VAGLIYAWRKGVLEWI"
     gene            2048..4375
                     /locus_tag="AC16V7_04740"
     CDS             2048..4375
                     /locus_tag="AC16V7_04740"
                     /inference="COORDINATES: protein motif:HMM:NF013961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HIR6256460.1"
                     /translation="MKITRPPGTSRWWDIPSILILLVILTTAFTRLSATNWSENLDAI
                     RMISYVGLLAGLALGYSRFSPRQVAFIALAYGIFTIPWRVGLSLGEEIAWMERLQSMA
                     GRLGVILWYLLQQRAAPDNFLFVTLMSILFWFTSVHAGYTLVRYANPWVVALPSGVAI
                     VLIQTYDPHPRRVWGLIIYLFLALLLVARLDFVNRRKRWEQNHTYIPSYLGTDFLRFA
                     LVACLLLIALSWATPALAKALPAAKTSWDRVVTPWWNDVRNVFENAFSSLRSSIGLTG
                     EYYGPNLSLGRGNILSDTVIFTVQAPGGIPAGVRYYWRARVYDTYEKIWISSLQTTRA
                     LDAQEIDLKFPDLSDNPPRTPSFTFYLSRPLATILAPNHPVWLSRPSRAEFSLNPDGT
                     VDLGMLRATPSLRGGEIYSVRSSFNVVTVEELRQAGTDYPSWVTARYLQLPTSITIRT
                     RQLAAQIAENKETPYDRAQAITQYLRENYQYTETVPALPSNQELIDWFLFDLKQGFCN
                     YYATAEVVLLRSLGIPARLAVGYASGENVDDPEFYTVRQLDAHAWPEVYFPEIGWVEF
                     EPTVSQPELSRPLTALGQDNLNNLSSSNLDREDPEPEPPTPRNTLDNPFERTNIWQER
                     LYIIIIAGLFAIMIALLIPLIRRKQLHKRIPFLSIALEKALLRAGLKPPAILSQIALM
                     ASLSPLERAYQQINLALVRLGKRPQPTHTPSERTTLLVNRLPSTREPAHLVLTEYQNT
                     IYSQHGLTNQIIAQRAGQEIRRISLRAYIRRRFGV"
     gene            4562..>5301
                     /locus_tag="AC16V7_04745"
     CDS             4562..>5301
                     /locus_tag="AC16V7_04745"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0140299 - molecular sensor activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR6256461.1"
                     /translation="MDSETSLAHILNQLITRLSHLTEASRQSINQDPTSLPTQIFDEF
                     GQLKNNLSKVSQHVALLEKHHKDLLSLINIGQVINSSLDPDEVLMTVMDTIIRLLKAE
                     RGFLMLKDTQGKLSIRVARNWTQETVQLSETAISRTVIKRVISDRKPVLTTNAQEDPR
                     FGGQESVIAYHLRSILCIPLKVKDELTGVIYADNRIHSGVFTEYELDLLSAFANQAAV
                     AIENARLFESLRQTLSEVTELKNLMDNV"
BASE COUNT         1411 a         1409 c         1187 g         1294 t
ORIGIN      
        1 caaataggga tgcaactaac caaagtgcag cgtgcaccag gttgcgcgtc gtgacgacaa
       61 gcagcgcaga gcctagggtt gccgcagcga caaggataaa aacaatttgc aaaggagtca
      121 tggacacact ccacttctat tgggctgtcc ctgcgggtgg ctgggaagca ctcgatggca
      181 ttacgtttgg ttgctgaggt ggtgtcgcca caggtcgcgg ttctcccacg gtttttcgtt
      241 caacccgtgc tcgggcgcgc aagatttgca aggtaaacca agcaatgaca atgtttgcca
      301 gcagcatcca cagggtgtag acgagcaaat tatcgcgagg cactaatttg tcggcgatgg
      361 cagtcactat caagaccacc agcgatagag gtgtaaggaa tttccaatta aaattcaaca
      421 tgtgatcaat gcgtatgcgc gggaaggtgt atttgaccca catgatcacc cagtacataa
      481 agaaagcttt gaggaaaaac cagaacaccc cgatgatcgg ataagtctct gcgccaaatc
      541 cacgccagcc accaaggaaa agagtagaga atattgctcc gaccgtcaag gcgtgcaaca
      601 gctctccagc gtagaacaaa ccgaatttca ttccagtgta ctcgatgtga aatccagcga
      661 cgatctcgga ctctgcctcg gtcaggtcaa acggcgcccg ccctaattct gcgatgttgg
      721 acaccaggaa aattagagct gctagtggcg ccatgaatag aaaccacacg gtttgaccat
      781 caacaatatc cttaagcccc atggagcggg ctaatatcac aggtaccagc aaagtgatca
      841 ccatgggaac ttcgtatgaa accatctgcg ctacagtacg aaatgcgcct agtagggcat
      901 atttattatt cgaagcccaa cccgccatga taattcccag cgtgcttatc gcaccgatag
      961 cgacaatata tagcacgccc acattgatat tgctgccata tattgtggct gagatgggca
     1021 gtactgccca caaggtaagc actgtgccca aggccataat tggggcgagg ttatagacaa
     1081 tcctgtctgc accaatcggg gtgatgtcct ctttaataag cagcttgatg atatcggcaa
     1141 ccggttggac caatccaaat ggccccagtc gatttggtcc cagacgatcc tggaaacggg
     1201 caactacctt tcgttcgacc cataccagaa aaatatccag caccaaagca actgttgcca
     1261 ttacaactac accgatcact gctaagatta aggtgacaaa accaggggca agcccccatc
     1321 caagcagcag gcctttcaac caactcgcga tgaatgttac tggatcaccc atccaaaaac
     1381 tccttatcct aaacacgtaa aaggctgagg gagataccca tcagcctttt tatacattag
     1441 catgaccaat atttctttag atccactcca atacgccttt gcgccaagca tatatcagcc
     1501 cagcgaccaa gatcaagata aaaagaacgc cttccaggac cgcgaacaat ggcaactggt
     1561 cgaaggctac tgcccatgga tacagaaaga cggtctcgac atcgaaaatg agaaatacca
     1621 gagcaaaaat atagtattgt acgcggaatt gtacccagga atcccccacc gtttcgatac
     1681 cgcattcata ggtctgtaat tttattgggt ttggcttgcg aggcgcaatc aaccggggaa
     1741 ccaccagggc taacccggga aaaatagggg cgagcaacaa aaatacgccg atgaataacc
     1801 agtcgttgag catgcaggct ccttatttag aagtatacgc ctattcccca agaaagtgag
     1861 aaggagaaca acggcgcaaa ttttaccata agttcaactg tttttacaaa gtttgttcca
     1921 tttttaacag attgtggatc tgtattcctg attaatgctt tcaaaacagg taatggagca
     1981 atgttaattg tacatcgaaa ataactatcg aaccaactac cgcttttttt ggtaaactga
     2041 atgaaagatg aaaattacgc gccctcctgg aacatcccgt tggtgggata taccgtcgat
     2101 tctcattctt ttggtcatat tgaccacagc ctttacgcgc ctatcggcaa ccaattggtc
     2161 tgagaacctc gatgccattc gcatgatttc ctacgttgga ttgcttgctg gattggcttt
     2221 ggggtacagc cgcttctcgc cacgccaggt agccttcatc gccctggctt acggtatctt
     2281 taccatcccc tggcgcgttg gtctctctct gggcgaggag attgcatgga tggaacgatt
     2341 gcaaagcatg gctggacggt taggtgtgat cctttggtat ttactccagc agagagccgc
     2401 gcccgataac tttctatttg tgaccttgat gagcatcctc ttctggttca ccagcgtgca
     2461 cgctgggtat acgctggtgc gttacgccaa cccttgggtg gtagccctac ccagcggtgt
     2521 tgcaattgtg cttatccaaa cctatgatcc gcacccgcgc cgtgtctggg gtctaattat
     2581 ctatttgttt cttgcgcttt tactagttgc ccgcttggat tttgttaatc gacggaagcg
     2641 ctgggaacag aaccatacct acataccatc gtatcttgga acggatttcc ttcgcttcgc
     2701 cctggttgca tgccttctcc taatcgcact ttcatgggca accccagcac ttgcaaaggc
     2761 actccccgct gctaaaacga gctgggatcg agtggtgact ccctggtgga acgatgtgcg
     2821 aaatgtattt gagaacgcat tttcctcact ccgctcctcg ataggcctga cgggggagta
     2881 ttatggcccg aatctctcac taggtcgtgg caatattctg agcgatacag ttatattcac
     2941 cgtccaagcg cctggtggta tacccgcggg ggtgcgctac tattggcgag cgcgcgtcta
     3001 cgacacgtat gagaaaatct ggataagctc gttacaaacc acacgcgcct tagacgccca
     3061 ggagattgac cttaaattcc ctgatctctc agataaccct ccacgaacgc cctcctttac
     3121 cttctatctg agtcgcccat tggctactat cttagctccc aaccatcctg tatggttgag
     3181 tcgcccttca cgcgccgaat tctccctcaa tcccgatggg actgttgatc taggcatgct
     3241 acgcgccacg ccttccttgc gtggcggcga gatttatagc gtacgttctt cattcaatgt
     3301 agttactgtg gaagaattga gacaagctgg cacagattac cccagctggg tcacagcacg
     3361 ctatctgcaa ttacccacgt ctattacaat acgcacccgc cagcttgccg cccagattgc
     3421 ggagaataaa gagacaccct atgatagagc ccaagccatc acgcaatatt tgcgtgaaaa
     3481 ctatcaatac actgagaccg ttccggccct gccatccaat caagaactga tcgactggtt
     3541 cttattcgat ctaaagcagg gattctgtaa ttattacgcc acagccgagg tcgtcctgct
     3601 ccgctctctc ggtattccag cccgccttgc tgtaggttat gccagtggtg agaacgtgga
     3661 tgatccagaa ttttatacgg tgcgccagct agatgcgcat gcttggccgg aagtttactt
     3721 tccggaaatc ggttgggttg aattcgagcc aacagtttcc caaccagaat tatctcgtcc
     3781 attaacggcg cttggacaag ataatctaaa taatttatcc agctcgaatc tcgaccgcga
     3841 ggatcctgag ccagagcccc ccaccccgcg taataccttg gacaaccctt ttgagcgcac
     3901 aaatatatgg caggaaaggc tgtatatcat tattatcgca ggtttatttg ctatcatgat
     3961 tgccctgttg atccccttga tccgcagaaa gcaattgcac aagaggatac ctttcctttc
     4021 aatcgcgtta gagaaagcct tgctgcgcgc aggactcaag ccgccggcta tactctccca
     4081 gatcgccctt atggcttcac tttctccatt agaacgagct taccagcaaa ttaacttggc
     4141 acttgtccgg ctgggtaagc gcccccagcc aacccatacc ccttccgaac gcacgacttt
     4201 gctggtaaat agattgccgt ccacaagaga acctgcccat ttagtgctaa ccgaatatca
     4261 aaacaccatc tacagccaac atggtttaac aaaccaaatc atcgctcagc gcgctgggca
     4321 agaaatccgc aggatttccc tccgagcgta catacgacgc cggtttggag tataaaaagg
     4381 ctctttggta acaaggtggt agaaataccc agctcggttg cgctgggtaa ttaatgtacc
     4441 ggatagcctg gcacttatgt ttgcactcgt ctgttgagct ttctggtaat agtcccagcg
     4501 aagtataata cctcatccta tttgttacgg aatttcccac ttcagtatgt cgagatggca
     4561 catggatagc gaaacatctt tagcgcatat actcaaccaa ctcatcacgc gactcagtca
     4621 cctaaccgag gcttctcgcc aatctataaa ccaagatccc acctctttac ccacccaaat
     4681 ctttgacgaa ttcggtcagc taaaaaataa cctctcaaaa gttagccagc atgtagccct
     4741 cctagaaaag catcataaag acctcctgtc actgatcaat atcggtcaag tgattaattc
     4801 ctccctcgat cctgacgaag tattgatgac cgtcatggac accatcatac gcttactgaa
     4861 ggcagagcgt ggctttctca tgctgaagga tactcaagga aagttatcta tccgcgtcgc
     4921 tcgcaactgg acacaagaaa cagtacaact ctcagaaact gcaatcagtc gcactgtgat
     4981 caagcgcgtt atttccgatc gtaagccggt gttaactaca aacgctcaag aagacccccg
     5041 ctttggcggt caagaaagtg taatagctta ccatctacga tctattctgt gcataccgct
     5101 caaggtaaag gatgaactca ccggggtaat ttatgccgac aatcgcatcc acagcggcgt
     5161 cttcaccgaa tatgagctgg atcttctatc cgctttcgcc aaccaagccg ctgtcgccat
     5221 cgaaaatgct cgcttattcg aatcgctgcg gcagactttg tctgaggtta cagagttgaa
     5281 gaacctgatg gacaatgtct t
//
LOCUS       DBNHOH010000267         6977 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_977221, whole genome shotgun sequence.
ACCESSION   DBNHOH010000267 DBNHOH010000000
VERSION     DBNHOH010000267.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6977)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6977)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6977
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_977221"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /locus_tag="AC16V7_04750"
     CDS             <1..1246
                     /locus_tag="AC16V7_04750"
                     /inference="COORDINATES: protein motif:HMM:NF024947.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HIR6256462.1"
                     /translation="LIESLYSQGTDMGDRFRANQVLLADTKQRVSQLEAEIDILSKAE
                     KEQLVQTQNIARLDTQLENIQQAIQDWEAVGAARLAEITSSLQEDAFSPEARDRLAEI
                     DAELKGIGYDAAAHDAARRKETDGRPADTAMRDLEKARAALSPLEREIADLQTQIKAL
                     KDEFTRQQDEHESARASLDQAEAQAPDVETAEIELLATQEQENQLRGDVGAAQQKVQV
                     LDTLKTRRKEYEAQREEIARQVGRYKQLERACGKDGVPALLIEQALPEIEGRANGILD
                     RLSGGSMSVRFVTQAAYKDRRREDLRETLDIQISDGAGTRDYEMFSGGEAFRVNFAVR
                     LALSEVLAQRAGARLQSLVIDEGFGSQDAQGRQRLVEAINLVRQDFAKILVITHIDEL
                     KDAFPTRIEVEKTDRGSTVRVT"
     gene            1270..2457
                     /locus_tag="AC16V7_04755"
     CDS             1270..2457
                     /locus_tag="AC16V7_04755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6256463.1"
                     /translation="MDLFLLTLSTIGLLVGLAITYWIHSQYRMEVVVRPSLSLSEINT
                     NPPLVSVIVPARNEDRNIRRCILALLAQDYPNFEVIVVDDRSTDETPRILADLQRSSA
                     NAHQRLIVIQGDELPTGWAGKPYALTQGAAVARGDWLCFVDADTFAIPELLSATYACA
                     QSQRADLFTMLTDQELGSFWEKSILPLVFTALSFGFPAARVNDPQKPDAIANGQFILI
                     RADVYHKLGGHEAVRGRIDEDKALAELVKRAGHRLVIADGRLVARTRMYTSLPEIWEG
                     WTKNIYLGLQDRLWLLLFGAVVGLLGALILPFWLLAGVVWLAAGGGIVAAIIAIQALI
                     VWSYLIVERMRVSRGMGISPLFAFTLPLGTLLFTAMMFVSAYNVLSGKGVTWKGRVYS
                     QSS"
     gene            2484..3224
                     /locus_tag="AC16V7_04760"
     CDS             2484..3224
                     /locus_tag="AC16V7_04760"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6256464.1"
                     /translation="MIVLETQQLTKQYQMGDVTVTALNDVNFAVERGEFVAIMGPSGS
                     GKSTLLHLLGGLDTPTSGEVVLAGQALSQLSDDQITRFRRQKIGFIFQFYNLLPTLTA
                     AENVVLPLLIDGRTASEYKARLQVLLSLVSLEDRASHLPDQLSGGQQQRVAIARAFIN
                     DPEIVLADEPTGNLDSRSGKSILDLLRKACRELEATIVIVTHDPRAASYADRVVFLKD
                     GQIVHRLNNGTEGVSVNKIVEVMASLEL"
     gene            3221..5818
                     /locus_tag="AC16V7_04765"
     CDS             3221..5818
                     /locus_tag="AC16V7_04765"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6256465.1"
                     /translation="MSTPFFLAQRNMRTRWGRTLLTLLGIVLGVAVVLAIQMTNQSTL
                     DSIRQVFDQATGQASLVVVPANRGADKLEEETLFRAEKFQGVLAAAPSVRVQTLLASE
                     AKSWQIAFSINGIAAGNLLVLYGVDPDLDTQVRVYKLAAGRMPLSDEYEAILPEKYAT
                     EKKLAIGDELEILTPSGRDSLEIVGLLADEGVALLNDGVVTFAPIQVVQDLFAMGSGM
                     DEIGLRLENAISEDPAALESFKDRLNERLGRDAEVIYPAGRGQLVSQMMATYQLGLSF
                     FSVIAVFVGAFLIYNTFSMTIVERTREIGMLRSIGMPRRGIIQMVLAEAILLSSVGSL
                     LGIGAGIVLARGLMQLVSEVIIPVENLARVPLEGLVQSLGVGVGVTLGAALIPATQAA
                     RISPLAALRVRTRSIERVRAAVWLSGLALLIVGRITLYHITWPTEIRFTVGSTSILLI
                     LLGATLTVALASGWLERLARPLAAALYGREGALGSANVRRSVSRTALTVASLMVALTM
                     IISINSLAYSFKEDMTAWIVNALGGDLYVRSPIVMRESFGRQLASVDGVQVVTPTRII
                     LVRAAQRSVPRDQSVGDTIVLNAIEPSTFRQISDMEFAAGQGNTQANWQRLGQGDAVF
                     ISNVVADRYQLDQGDTLYLLTRRGEKGFYIAGIVMDFTGQGLVIYSTYLVLQRWFGET
                     GADRFTIKTAQGSSVAVVAEEIEERYQKRRNISVQTTEEFKDNILNLMDRSFRLFDVL
                     NLIGVIIGALGVINTLTMNVMERQREIGGLRSLGMTRRQVLRMVLAEAQALGIMGGIY
                     GLGFGYAIAHVLILGTNLMIGYDLVFLFTAQPYIIGALIALVIVQMAAIYPARRAAHI
                     NIVEAIKHE"
     gene            complement(5888..6082)
                     /locus_tag="AC16V7_04770"
     CDS             complement(5888..6082)
                     /locus_tag="AC16V7_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256466.1"
                     /translation="MAYSFKNSRGVTYYLHGKTRTLSSGKTTTLYYFAKAVQAGALDA
                     VPAGYVVSESKNGLPVLKRK"
     gene            6427..>6977
                     /locus_tag="AC16V7_04775"
     CDS             6427..>6977
                     /locus_tag="AC16V7_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256467.1"
                     /translation="MTKESPNNQIFRSTRLISAVVVPFLVLAFIILYFYPNLSGERFA
                     WKIQPNMTAVFMGAGYLGGAWLFINAIFGRRWHHIAAGFPPVTTFTVAMLLATALHWD
                     RFDIRHFPFILWLGLYVITPFLVPWMWLRNRVTDPGIPEANDLLVPVVARWSLRLLGV
                     FLFIFAVVGFIAPQWVIGLWPWQL"
BASE COUNT         1484 a         1952 c         1999 g         1542 t
ORIGIN      
        1 gctgatcgaa agcttgtaca gccagggcac agacatggga gaccgcttcc gagccaacca
       61 ggttttgctt gcagacacca aacagcgggt cagccagctg gaggcggaaa ttgacatcct
      121 tagcaaggct gaaaaagagc agctcgtgca aacccagaac atcgcccgcc tggacaccca
      181 gctagagaac atccagcaag ccatccagga ttgggaggcg gttggagcag cgcgcctggc
      241 agagataacc tccagcctcc aagaagatgc tttcagcccc gaggcgcgcg accgattagc
      301 cgagatcgac gctgagctca agggtattgg ctacgatgcc gcagctcacg acgctgcccg
      361 ccgcaaggag actgacggtc gcccagcaga cacggccatg cgcgatcttg agaaggcgcg
      421 ggccgccctc tctccactgg agcgcgagat cgccgacctg cagacgcaga ttaaagccct
      481 aaaagacgag ttcacccgcc agcaagatga acatgagagc gcacgcgcct ccctggatca
      541 ggctgaagcg caggcgcctg acgtggagac cgctgagatt gagctgctgg ctacccaaga
      601 gcaagagaac cagctgcgcg gagatgtggg tgcggcgcag caaaaggtgc aggtactgga
      661 tacactcaag acgcgccgta aggaatacga agcacaacgc gaagagatcg cccggcaagt
      721 aggtcgctac aaacaactgg aacgcgcctg cggcaaggat ggcgtgccag ccctgcttat
      781 tgagcaagcc ctacccgaga ttgaggggcg cgccaatggc atcctagacc gcctaagcgg
      841 aggtagcatg tcggtgcgct tcgtcaccca ggcagcttac aaggatcgcc gccgggagga
      901 tctgagggag accctggaca tccagatctc tgatggtgct gggacgcgcg attacgagat
      961 gttttccggt ggcgaggctt tccgcgtcaa ttttgccgtc cgcctggcgc tctccgaagt
     1021 gctggcacaa cgcgccggag cccgcttgca atccctggtg atcgacgagg gtttcggcag
     1081 ccaggacgcc cagggccgcc agcgcctggt ggaggctatc aacctggtgc gtcaggattt
     1141 tgccaaaatc ctggtgatca cccatatcga tgaactcaag gacgcgtttc ccacacgtat
     1201 cgaggtagag aagaccgata gggggtcgac tgtgcgcgtg acataaagca cgcatttact
     1261 gtattaccaa tggacctttt cctcttgaca ctttccacaa tcggattatt ggtcggtctg
     1321 gcgatcacgt actggatcca cagccagtac cgcatggagg tcgttgtccg tccatcgctc
     1381 tccctctcag agattaacac caacccacct ttggtatccg taattgtgcc agcccgcaac
     1441 gaggatcgta acatccgtcg ctgcatcctg gcgttgctgg cacaggatta cccgaatttt
     1501 gaggtcatcg tggtggacga ccgctcgacc gacgaaacgc cgcgcatcct tgcagacctg
     1561 cagcgctcct cggcaaacgc ccatcagcgc ctgattgtga ttcaaggtga cgagctccct
     1621 acaggctggg caggcaaacc atatgccctg actcagggag ctgctgtagc gcgcggcgac
     1681 tggctatgct tcgtggacgc agataccttc gccatccccg aactactctc cgcaacatat
     1741 gcctgtgcac aaagccagcg cgccgacctg ttcacgatgc tgactgacca ggagctgggc
     1801 agcttttggg aaaaatctat cctgccactg gtcttcacag cactgtcttt cggctttccc
     1861 gcggctcgcg tcaatgaccc gcagaaaccc gacgctatag ccaacgggca gtttatcctg
     1921 atcagggctg atgtttacca taagctaggc gggcacgagg ccgtgcgcgg gcgcatcgac
     1981 gaggataaag ccctggctga actggttaag cgagccgggc accgcctggt gatcgccgac
     2041 ggtcggttgg ttgcgcgcac gcgcatgtac acctcgctgc ctgaaatttg ggaaggttgg
     2101 acgaaaaaca tctaccttgg cttgcaagac cgcctgtggc tgctgctttt tggcgcggtg
     2161 gttgggctgc tgggggcact gatcctgcct ttttggctgc tggccggagt agtctggctt
     2221 gccgccgggg gaggcatcgt ggcggctatc attgccatac aggctctgat tgtctggagt
     2281 tacctgattg tcgagcgaat gcgggtttca cgaggtatgg gcatctctcc cttatttgcc
     2341 ttcaccttgc cgctggggac attgctcttc acagccatga tgtttgtttc cgcatataac
     2401 gtgctttcgg gcaagggggt cacctggaag ggacgagtat attcccaaag ctcataaata
     2461 aaactgcatc aatttattgt ccaatgatcg ttcttgagac tcaacaactt accaaacagt
     2521 atcaaatggg ggatgtgacc gtcaccgcgc taaacgatgt taactttgct gttgagcggg
     2581 gcgagttcgt cgccatcatg gggccatcag gctctgggaa atccactttg ctgcacctgc
     2641 tgggcggcct ggatacgccc acttcaggcg aggtcgtcct ggcaggacag gcgctctcgc
     2701 aattgagtga cgaccaaatc acccgctttc gccggcagaa gatcggcttc atcttccagt
     2761 tttacaacct attgcccacg ctgaccgccg ccgagaacgt ggtactcccg ctgcttatag
     2821 acggacggac ggcgtcagaa tacaaagcac gccttcaggt gctgttatcg ctggtgagcc
     2881 tggaggaccg cgccagccac ctgcccgacc agctttccgg tgggcagcag cagcgggtgg
     2941 ctattgcgcg tgccttcatc aatgatccgg agatcgtgct ggccgacgag cccaccggca
     3001 atctcgactc gcgctcgggc aagtccatac tggatctgct gcgcaaggct tgccgcgaac
     3061 tggaggcgac catcgtcatc gtcacccacg acccgcgcgc cgcttcctat gccgaccggg
     3121 tggtattcct caaagatggg caaatcgtgc accgtctgaa caatggcaca gaaggtgtgt
     3181 ccgtcaataa aattgttgaa gtgatggcgt ctttggagct atgagtacac ctttcttcct
     3241 tgctcagcgg aatatgcgca cgcggtgggg gcgcacgctg cttactctgt tgggcattgt
     3301 gctgggagtg gcggtcgttt tagccatcca gatgacaaac cagagcaccc tggattcgat
     3361 ccgccaggtg ttcgaccagg cgactggaca ggccagcctg gtggtggtac cagccaaccg
     3421 tggtgcagac aaactagagg aggagacgct cttccgcgcc gagaagtttc agggcgtgtt
     3481 ggcagcggca ccctccgtgc gcgtgcaaac cttgctagcc agcgaggcca aatcctggca
     3541 gatcgccttt tcaatcaatg gcatcgccgc aggcaattta ctcgtgcttt acggcgtgga
     3601 ccccgacctc gatactcagg tacgtgtcta taaacttgcc gctggacgca tgccgctctc
     3661 tgacgaatac gaagccatct tgccggagaa atacgccaca gagaaaaagc tcgcgatagg
     3721 tgatgaactt gagatcttga ctccatcggg acgcgatagc ctggagattg tgggcttgct
     3781 ggctgacgaa ggcgtggcgc tgctcaatga tggggtggta acctttgcgc ctatacaggt
     3841 tgtgcaagat ctgtttgcaa tgggcagcgg gatggacgag atcggcttgc gcttggagaa
     3901 cgccatcagc gaagatccag ccgcactgga atcgttcaaa gaccgcctga acgaacgcct
     3961 ggggcgggat gccgaggtga tctaccctgc aggtcgcggg cagcttgtca gccagatgat
     4021 ggccacctac caattaggct tgagcttctt ctccgtcatc gccgtgtttg tgggtgcctt
     4081 cctgatctac aacacattct ccatgaccat cgtggagcgc acgcgggaaa ttggcatgct
     4141 gcggtcaatt ggcatgcccc gccgaggcat catacagatg gtactggctg aggccatatt
     4201 gctttcaagc gtgggatcgc tgctgggcat cggcgcgggg attgtgctgg cgcgcggctt
     4261 gatgcaactg gtcagcgaag tgattattcc ggtggagaac ctggctaggg tgcccctaga
     4321 ggggctggtg caaagcttag gcgtgggggt tggagtgact ctcggagcag cactgatccc
     4381 ggcaacgcag gcggcgcgca tctcgccatt ggcggcccta cgagtgcgca cccgcagcat
     4441 agaacgagtc cgggctgcag tgtggctctc cgggctagcc ctgctcattg tagggcggat
     4501 cacgctctat cacatcacat ggccaacaga gatacgtttt accgtgggca gcacatcgat
     4561 cctgctcatt cttctaggcg ctactttgac agtggccctg gcaagcggat ggcttgagcg
     4621 gctcgcacgc ccgctagctg cagccttata tgggcgggag ggagccctgg gctcggcgaa
     4681 tgtgcgtcgc tcggtaagtc gaacagcgct aaccgtcgcc tcgctgatgg tagcgttgac
     4741 catgatcatc agtatcaatt cgctggcata ttccttcaaa gaggacatga ctgcctggat
     4801 cgtgaatgcc ttaggtggcg acctgtacgt gcgctcgcca atcgtcatgc gcgaatcttt
     4861 tggacgccaa ctcgccagcg tggacggtgt gcaagtggtt acgcccacgc gcatcatcct
     4921 ggtacgagct gcgcagcgct ctgtgccgag ggatcaatct gtgggtgaca cgatcgtcct
     4981 gaatgctatc gagccaagca ccttccgcca gatcagcgac atggagttcg ctgctggtca
     5041 gggtaatacg caagcaaatt ggcaacgatt ggggcaagga gatgcagtgt tcatctccaa
     5101 tgtagttgcc gaccgctacc aactggacca gggtgacacg ctttaccttc tcacccgacg
     5161 tggggaaaaa ggcttctata tagccgggat cgtaatggat ttcaccggcc aagggttggt
     5221 gatttatagc acctatcttg ttttacaacg ctggttcgga gaaactggtg ccgatcgttt
     5281 cacgatcaag actgcgcagg gaagctctgt agctgtggtc gcggaggaga tcgaggagcg
     5341 ctaccaaaaa cggcgcaaca tcagcgtaca gactacagaa gagttcaaag acaacatcct
     5401 gaacctgatg gaccggtcct tccgcctgtt tgacgtgctt aacctgattg gggtgattat
     5461 cggggcactg ggggtgatca atacgctgac catgaacgtg atggaacgcc agcgagagat
     5521 cggcggcctg cgctcgttag ggatgacccg ccgccaggtg ctgcgtatgg tgctggcgga
     5581 ggcacaggcg ctgggcatca tgggcggcat ctacggactt ggcttcggtt acgccatcgc
     5641 ccacgtgctg atactgggta ccaatctgat gattggctac gacctggttt tcctgttcac
     5701 agcccagccc tatatcatcg gcgctctgat cgctttagtg attgtacaga tggcggcgat
     5761 ctaccccgcg cggcgggcag cgcacatcaa cattgtcgag gcgatcaagc acgagtaaat
     5821 atacgcgcta aaactagggt ggctcattgt gagccaccct ttccttcaga gaactagatt
     5881 gattggttta cttgcgcttt agaactggca acccgttctt gctttcggat acgacgtacc
     5941 ctgctggtac ggcgtctaat gcacccgcct ggacagcttt cgcgaaataa taaagcgtcg
     6001 tcgttttacc gctcgaaagg gtgcgagtct ttccatgcag ataataggtt acaccgcgtg
     6061 agtttttgaa actgtaagcc atgattatcc tccttccaat atctgtcgaa ctatattgca
     6121 gttaagtgaa aattgatcca ttgatagata gtccaaatag ccttctctca caccttacaa
     6181 ctatacttta tctcgtttta ttcagcccgt caatggttga atttgcccct ttcaaggggt
     6241 ggtgaacgca ttttctaggt cgttccgatg cgtctccgac tctcttaacc attataattg
     6301 ggggtcatgg aggcaatccc agtacccaga ctgcgtggta catctgagcg cgcatccttt
     6361 caaagattaa ggcgtttaac atatatacat caaaccgaat cttagcttta caggtattca
     6421 aaaacaatga cgaaagaatc tccaaataac caaatcttcc gctcaacacg cctcatctcg
     6481 gcggtggtcg tgcccttcct ggtattggcc tttatcatcc tatacttcta ccctaatcta
     6541 tcaggtgagc gcttcgcatg gaagatccag ccaaatatga cggctgtctt catgggggcg
     6601 ggttatcttg gcggcgcatg gctattcatt aacgccatct ttggccggcg ctggcaccac
     6661 atcgctgcag ggttcccgcc ggtgacaacc ttcacggtcg ccatgctcct ggcaactgcg
     6721 ctccattggg accgcttcga catcagacat tttcctttca tcctgtggct gggcctgtat
     6781 gtcatcacgc catttctcgt cccttggatg tggctacgca accgcgtaac tgatccgggc
     6841 attcccgagg ccaacgatct gctggtccca gtggttgcac gctggagcct gcgattgcta
     6901 ggcgttttcc tgttcatctt cgccgtggtt gggtttatcg ccccgcagtg ggtcatcggc
     6961 ttatggccat ggcagct
//
LOCUS       DBNHOH010000268         3222 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1682576, whole genome shotgun sequence.
ACCESSION   DBNHOH010000268 DBNHOH010000000
VERSION     DBNHOH010000268.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1682576"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1227)
                     /locus_tag="AC16V7_04780"
     CDS             complement(49..1227)
                     /locus_tag="AC16V7_04780"
                     /inference="COORDINATES: protein motif:HMM:NF017072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-fold NAD(P)-binding domain-containing
                     protein"
                     /protein_id="HIR6256468.1"
                     /translation="MQINIGLARMHMEPGERRDFLPGFVARLEHYGARVVLEYGYGSG
                     MTLVEEDYLQVAPSVTFATRQEVYQQDIVLVLRCPDTNDISMLRPGACFISMLHYPTR
                     PQRVEFLRSLGIEAVSLDSIKDDSGRRVVENLRSVAWNGIEAAFKVLRNTYPDPGFGS
                     KQRSPIQVTLMGTGAVGSHVVSAASRYGNENLRAKMVAAGIPGVKVVAIDYDVTPHEE
                     IMREILMNTDILVDATQRPDPSKPVIPNQWVGYLPQHAVILDLSVDPYNCDTQLLSIK
                     GIEGIPQGNLDRYIFSPDDPEFDILPACISTIHRRHTVSCYSWPGIYPKKCMELYGRQ
                     IRPILHVLIDKGGPQNIDPEGSFFERAIARALLSRLPIDNNAVQSTQYSQQRNNKDGS
                     "
     gene            1404..1973
                     /locus_tag="AC16V7_04785"
     CDS             1404..1973
                     /locus_tag="AC16V7_04785"
                     /inference="COORDINATES: protein motif:HMM:NF036525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6256469.1"
                     /translation="MTRDEILEAAAQIFREKGFHAASMQDIALAVNLQKASLYHHVSS
                     KQEILLALLDRALDLLIEHIEQVISQPLSPDEKLRQAMRVYLQVMLENRDLAAVLLLE
                     HRSLEPQYHARHTPRRDRFERLWRDLINDGHEQGIFSCADPALASRFVLGVLNWSITW
                     YRPDGSLSPTEIADQFAEMFLKGLLVRKS"
     gene            1973..3043
                     /locus_tag="AC16V7_04790"
     CDS             1973..3043
                     /locus_tag="AC16V7_04790"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6256470.1"
                     /translation="MMVEEPLSKPPEVSLFETSRGARIFQIPLQEFPILWGYAYLVLL
                     DDPQEGSYRVLIDSGSGFGDSNQHLEYGFQRISQIIGQTIGLEHLTHVFITHGHIDHF
                     GGLTYVRPRTQARIGVHELDLRNLTNYEERLTIVARRLEAFLFEAGVSPGRHANLIEM
                     YMLPKSLFHSIHVDFTYEAAGMRVGPFEFLHVPGHCAGHVVIRLHDVLFSGDHVLDGL
                     SPHQSPEHLTLFTGLDHYLKSLDALEAWAGDARITLGGHRGVITDLPGRLNAIRQVHT
                     KRLNSVLEILEQPHTISEVSHALFGDVHGYNVLLALEETGAHVEYLAQRGRLRISNLD
                     EIEKNHNPVAIRYQKLALNGEE"
BASE COUNT          753 a          805 c          867 g          797 t
ORIGIN      
        1 tagttgcacc tcgtaataag atttgagaat gatgttggac ctacctggtc aggagccatc
       61 cttgttgttg cgttgctgag agtattgtgt gctttggacc gcgttgttat ctataggtaa
      121 gcgagagagc aaagcgcggg caatggcgcg ttcgaaaaag ctgccctcgg gatcgatatt
      181 ttgcggtccc cctttgtcta tcaggacatg caaaatgggg cggatttgcc gtccgtacaa
      241 ttccatgcac ttctttggat aaatgccagg ccacgagtaa caagataccg tgtggcggcg
      301 atggatggtt gaaatgcaag ccggcaggat gtcaaactct gggtcgtctg gagagaagat
      361 atagcggtct aggttgcctt gagggatacc ttcaatacct ttgatggaaa gcagctgcgt
      421 atcacagtta taaggatcta ccgagaggtc gagaatgact gcatgttgcg gtaaataacc
      481 cacccattgg ttcgggataa cgggtttact tgggtcgggg cgttgggtgg catccactaa
      541 gatatcggta ttcatcagga tctcgcgcat gatctcttcg tggggcgtga catcgtaatc
      601 gatggcgacc actttaactc ccgggattcc cgcagccacc attttcgcgc gcaggttttc
      661 attgccatag cggctagctg ctgaaacaac gtgactgcct accgcacctg ttcccatcaa
      721 tgtgacctgg atgggggagc gttgcttgct gccgaaaccg ggatcgggat aggtgttgcg
      781 taatactttg aaagcagcct cgatgccgtt ccaggctacc gagcgcaggt tctccaccac
      841 tcgtcgccca gaatcatctt tgattgaatc cagggacacg gcttcaatac ccaaagagcg
      901 taggaattca acccgctgag gacgtgttgg ataatgtagc atagaaatga agcatgcgcc
      961 tggtcgaagc atggagatat cgttggtatc gggacagcgc aggaccagta cgatgtcttg
     1021 ttggtaaacc tcctggcggg tagcaaaagt aacggacgga gcgacctgga ggtagtcctc
     1081 ttcgactagt gtcatgccgc tcccatagcc atattcgagc acaaccctag cgccataatg
     1141 ctccagcctg gctacaaatc cgggcaagaa atcgcgtcgc tcgcctggtt ccatatgcat
     1201 gcgcgccagg ccgatattga tctgcatttg cgtagttctc cttcgttcct gtgataacga
     1261 actcagttta ccacagctta atatggcaca agtaacgatt ggactatgca ggaggcatac
     1321 ataccatgtt gtaagttgac attagggttg gtttttgata tactaaatct accgaccgtt
     1381 cggtagggag taaaggaaaa gttatgaccc gggatgagat cctggaagct gccgctcaaa
     1441 tctttcgtga aaaaggtttc catgccgcct cgatgcaaga tatcgccctg gcagtaaacc
     1501 tgcagaaagc cagcctttat caccacgttt cgtccaagca agagatatta ttggcgctgc
     1561 tcgatcgggc gttggacctg ctaattgagc atatcgagca ggtgatctcg cagccgctct
     1621 ctcccgatga aaagctccgc caggcaatgc gcgtatattt acaggtcatg ctggaaaatc
     1681 gtgatctggc tgcagttttg ctcctggaac atcgctccct tgagcctcag tatcacgccc
     1741 gtcacacacc gcggagagac cgttttgagc gcctgtggcg ggatttgatt aatgatggtc
     1801 acgagcaggg cattttttcc tgcgcagacc ctgccctggc ttcccgcttt gtgctgggtg
     1861 tcctgaattg gtccatcact tggtaccgcc cagatggttc cctttcaccc actgagatcg
     1921 ccgatcaatt tgccgaaatg tttttaaagg gtttactggt ccgtaaaagc tgatgatggt
     1981 tgaagaaccg ctatccaaac cccctgaggt aagtctattt gagacttccc gtggcgcgcg
     2041 tatatttcaa attcccctcc aggagtttcc catcctgtgg ggttatgcct atctggttct
     2101 actggatgac ccccaggaag gctcttatag ggttttgatt gatagtggct cgggttttgg
     2161 cgactcgaac cagcatctgg agtatggatt ccagcgaata tcccagatta tcgggcaaac
     2221 gattggcttg gaacacctga cccatgtttt tatcacccat gggcatattg accacttcgg
     2281 gggactcacc tacgtccgcc cgcgcacgca agccagaatc ggggtgcatg agcttgacct
     2341 gcgcaacttg accaattacg aagaacgttt gacaatcgtc gctcgtcgcc tggaggcatt
     2401 cttgtttgaa gccggtgttt cgccggggcg gcacgccaac ttgattgaaa tgtacatgct
     2461 gccgaagagc ctgttccatt ccatccatgt ggatttcacg tacgaagcgg caggcatgcg
     2521 cgtaggacca tttgagtttt tgcatgtacc tggacattgc gcgggtcacg tggttatccg
     2581 cctgcacgac gtgctgttca gcggcgacca tgtgctagat ggccttagcc cacaccagtc
     2641 gccggagcat ctaacgctat ttaccgggtt ggatcattat cttaaatcac tggacgccct
     2701 ggaagcctgg gcgggcgacg cacgcatcac actgggaggg caccgcgggg tgatcacaga
     2761 tctgcctggc cgccttaatg ccattcgcca ggtgcacaca aaacgattga attcggtgct
     2821 ggaaatattg gagcagccgc acaccatctc tgaggtttct catgccttat ttggcgatgt
     2881 gcatggctat aacgtattgc ttgccctgga agagaccggt gctcatgtgg aatatctggc
     2941 ccagcgaggc cggctgcgga tcagcaacct ggatgaaatc gagaaaaacc acaacccggt
     3001 tgctattcgc taccaaaaac tagcattaaa tggggaagaa tgacttggat ttgtcgagtt
     3061 aatctgatga gaaagtagat ccagaaggag tgtgaccatg ttctcatttg aaccaactga
     3121 agagcaaaaa atgctcgtgg acgccattgg gcgttacgcc caaagtgact tgcgcgccaa
     3181 ggctcatgat gccgatgaag aagaccagta cccgcaaagc ct
//
LOCUS       DBNHOH010000269         1938 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_335426, whole genome shotgun sequence.
ACCESSION   DBNHOH010000269 DBNHOH010000000
VERSION     DBNHOH010000269.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_335426"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1353)
                     /locus_tag="AC16V7_04795"
     CDS             complement(<1..1353)
                     /locus_tag="AC16V7_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256471.1"
                     /translation="MRRLHLVSLAAVWLLMMAVVACAGAPTPAAPTSPPTSAPSPTQS
                     ESISDETEDLEKPYEDFDPDNFDDPTTIDNEWLPMRPGMQHIFEGVTEEAGLTIPHRV
                     IFTVTDLTKVIDGVRTVVIWDQDFSAGALVETELAFFAQDNDGNVWRMGEYPEVYEDG
                     KLVEAPAWISGLKGAVAGIMMKADPQLGMPSHSQGWAPSVDFTDRGQVDQVGQETCVP
                     AGCYEDVLVVAEFSEAEPDAFQLKYYAEDLGNIRVGWRGADATKETLELVEIIQLSPE
                     ELVEIRAQALELEERAYEISEEVYDQTPPIEIPQDSASVPEPTQGTVTDGATAEVLVY
                     ASDLPESALSEFEVWDEPVSPGGKMLGVTNNGDEMDPPPEDDPHVTFNVQVQDSIPYR
                     CWIHMKVGAPKGKSQANVFFVQFSDAMKQSNEDAFAPETDSYLTAQGPTQEGWTWVGC
                     D"
     gene            complement(1377..>1938)
                     /locus_tag="AC16V7_04800"
     CDS             complement(1377..>1938)
                     /locus_tag="AC16V7_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6256472.1"
                     /translation="AERQAASQRILLAGSVAVIAIFFLLYTSFENWRLATLTFFTLPW
                     ALVGGVLAAYFSNGILSLGSLVGLLTILGIATRNGIMMISHFQHLEEEEGETFGPELV
                     KRGARERIAPIMMTALTTGLALVPLAIAGEIPGQEIEYPMAIVILGGLVTSTLLNLLV
                     LPTMYLRFGKSEAWQSPSQSSAGSTA"
BASE COUNT          448 a          551 c          497 g          442 t
ORIGIN      
        1 atcacagcca acccaagtcc agccttcctg ggtcgggcct tgcgcagtca ggtagctgtc
       61 cgtttccgga gcgaaggcat cttcatttga ttgtttcatg gcatcagaga attgcacgaa
      121 aaacacgttc gcttgcgatt tgcccttcgg tgcgccaact ttcatatgta tccagcaacg
      181 gtaagggatg ctatcttgaa cctgcacatt gaaagtcaca tgggggtcat cctcgggagg
      241 agggtccatc tcatcgccgt tattggtaac tccgagcatc ttacctcctg gtgatactgg
      301 ctcgtcccaa acttcaaatt cggataaggc gctttctggt aggtctgagg cgtagaccaa
      361 aacctccgcc gtggcaccgt ccgtcactgt accctgggtg ggctcaggaa ctgaggccga
      421 atcctgggga atttcaattg gcggagtttg atcgtatacc tcttcgctga tctcgtaggc
      481 gcgctcctcc aattccagtg cttgtgcgcg aatctctact agctcttccg ggctgagttg
      541 gatgatctca accagttcta gtgtctcctt ggttgcatcc gcacccctcc agccaacacg
      601 tatattgccc aaatcctcag cgtagtactt gagctggaat gcatcgggct ccgcctcgct
      661 aaactctgca accaccagca catcttcata acaacccgct gggacacagg tctcctgacc
      721 tacctgatct acctgcccgc ggtcagtgaa atccactgag ggtgcccatc cctgggagtg
      781 actgggcatc cctagctggg gatccgcttt catcataatc cccgctaccg cgcctttgag
      841 acctgaaatc caggcaggag cctcaacgag ctttccgtcc tcgtatacct ctggatactc
      901 acccatgcgc cagacattgc catcattgtc ctgcgcaaag aaggccagct ccgtctccac
      961 caatgcaccc gcgctgaagt cctgatccca aatgactaca gtacgcacgc catcaatcac
     1021 cttggtcagg tcagtgacag tgaatatcac gcgatgaggg atagtcagac cggcttcttc
     1081 tgtgacccct tcaaagatgt gctgcatccc cggtcgcatg ggtaaccact cgttgtcgat
     1141 ggtagtcgga tcatcgaaat tgtcggggtc gaagtcctca tatggtttct caagatcttc
     1201 ggtctcatcg gagatggatt ccgattgtgt aggtgatggt gcagatgtgg gtggcgaggt
     1261 cggtgcagcc ggggtaggcg cgccagcaca tgccacaacc gccatcatga gaagccacac
     1321 tgctgctaaa gagactagat gaaggcgtcg catagtaatt cgtattcctt tcaaaattaa
     1381 gctgtactgc ctgcgctaga ttgggaaggc gactgccatg cttcgctctt cccgaagcgc
     1441 aaatacattg tgggtaatac cagcaaattc agcaatgttg aagtgaccag gccgcctaga
     1501 atgacgatgg ccatggggta ctcaatctcc tgaccaggaa tttcaccagc gatcgccaag
     1561 ggtacaagag ccagccctgt ggtgagagct gtcatcatga tgggggcaat ccgctcgcgc
     1621 gccccgcgct taactagctc gggaccaaag gtctcgcctt cctcctcctc cagatgttgg
     1681 aagtggctga tcatcataat cccgttgcga gtggcaatac caagtatggt gagcaacccg
     1741 accaaggaac cgagcgaaag gatgccgttg ctaaaatagg ctgccagcac gccacctacc
     1801 aacgcccaag gcaatgtgaa gaaagtaagc gttgccaagc gccagttttc gaaagaggtg
     1861 tacagaagga agaaaatcgc gattacagcc acagatccag ccaggaggat acgctgcgaa
     1921 gctgcttgcc tctcggca
//
LOCUS       DBNHOH010000270         1836 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_207788, whole genome shotgun sequence.
ACCESSION   DBNHOH010000270 DBNHOH010000000
VERSION     DBNHOH010000270.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_207788"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..1472
                     /locus_tag="AC16V7_04805"
     CDS             204..1472
                     /locus_tag="AC16V7_04805"
                     /inference="COORDINATES: protein motif:HMM:NF017019.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HIR6256473.1"
                     /translation="MNIRSITYFCDPGWPLDGEQLRQAGSFMAAARPAFESAGYPVQT
                     TRLASVPFPRLVNGWGTAQQTSERASPVEHVVELAQALDESVAAQGIDYISLGPALPE
                     VPESYTAIPAALSATQNTFLSGSMTGVDGRISLPAVRQCAKVISEAANISPDGFANLR
                     FAALANVPAGAPFFPAAYHEGGAPVFALAMEAAVLAVEAFDGAPSLHAARQNLVEAME
                     SHARAMEVVAQKLAESHSLRFGGIDFSLAPFPEEQRSLGTALERLGVPAVGLHGSLAA
                     AAFLTDSVDRARYPRAGFSGLFLPVLEDAVLARRAAEGKLSVNDLLLLSAVCGTGLDT
                     VPLPGDVDADELAAVLLDIAALACRLDKPLTARLMPIPGKRAGDLTSFDFSYFATSRV
                     LALDARPLQGLLVGEESFDLKPRPPSGSEK"
     gene            1597..>1836
                     /locus_tag="AC16V7_04810"
     CDS             1597..>1836
                     /locus_tag="AC16V7_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6256474.1"
                     /translation="MARIVKEEEYIARRNQILDAAQRLVYTKGYEQMTIQDILDDLQI
                     SKGAFYHYFDSKGEVLETLVERMVVDEVIPLLTNIV"
BASE COUNT          356 a          519 c          575 g          386 t
ORIGIN      
        1 ggcgcgcctg catattccta tggccggcgg agtggaatcg ttgaacgccg cggccgcagc
       61 ggcagtgctg ctcttcgaag tcgtccggca gagaactcag gccgggaaat aatccgaaga
      121 ggaagtatgc gcgcacaagg cgcccgcata cctccccttc gtaattgttg ccccctccaa
      181 tgggaagggg ggatgggcta ataatgaata ttcgatccat cacttatttt tgtgatccag
      241 gctggcccct ggatggtgaa cagttacgcc aagcggggag cttcatggcc gcggcgcggc
      301 cggcgttcga gagcgctggc tacccggtac agacaacgcg tctggcgagt gtgccattcc
      361 cgcgtttggt gaatggctgg ggtactgctc agcaaacttc agagcgtgcc tcgccagtgg
      421 agcacgtcgt ggaattggcg caggcgctgg atgaaagtgt tgccgctcag gggatcgact
      481 atatttccct cggtccggct ctcccggaag ttccggagag ctacactgcc atccccgcag
      541 ccctgagcgc aacccagaac actttcctga gcgggagtat gactggcgtc gatgggcgca
      601 tatcattgcc ggcggtgcgc cagtgcgcca aggtcatctc tgaggcggcg aacatctccc
      661 cagacggctt tgccaatctt cgctttgccg ctttagccaa cgtgccagct ggcgcgcctt
      721 tcttcccagc cgcttatcat gagggcggcg cgccggtttt cgccctggcg atggaggcgg
      781 ctgttctggc tgtggaggct tttgacggtg cccccagcct gcacgccgcg cgccagaatc
      841 tggtggaggc gatggagagc catgcccgtg ccatggaggt cgttgcccaa aagctggccg
      901 aatcgcacag cctgcgcttt ggcggcattg atttctcgct agctcctttc ccggaagaac
      961 agcgctcgct ggggactgcc cttgagcgcc tgggtgtccc ggcggtcggg ctgcacggca
     1021 gcctagccgc ggcggcattc ctgaccgact cggtggatcg cgctcgctac ccccgcgccg
     1081 gattcagcgg gctattcctg ccggttcttg aagacgccgt gctggcgcgc cgtgccgctg
     1141 agggcaagct gagcgtcaat gatttgctgc tcttatctgc ggtgtgcggg acgggactag
     1201 ataccgtgcc gctaccaggc gatgtggacg cggacgagct ggcggctgtg ctcttagata
     1261 tcgccgccct ggcttgccgc ctggataagc cactcaccgc ccgcttgatg cccatcccag
     1321 gcaagcgcgc tggcgacctg accagctttg atttctctta tttcgccact agccgcgtgc
     1381 tggcgctaga tgcccggccg ttgcaggggc tgctggtagg ggaggagagt ttcgatctca
     1441 agccgcgccc acccagcgga tcagaaaaat aatcttgcaa ggaatcagga taataaggcc
     1501 aacggactct gagtggggat tgacagggtg tagcgcgaag gatataattt ttattaaaat
     1561 gataaaccgt cggtcggttt aataaagaga tgaataatgg ctcgcattgt taaagaagag
     1621 gaatacatag caaggcgtaa ccaaatcctg gacgcagccc agcggctggt gtataccaaa
     1681 ggttacgagc agatgacgat ccaggacatc ctagatgacc tgcagatctc caagggggcg
     1741 ttttatcatt attttgactc gaaaggggag gtgctggaaa cgctcgtcga gcgcatggtc
     1801 gtggatgagg tcataccact gctcaccaat atcgtg
//
LOCUS       DBNHOH010000271         1384 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_143233, whole genome shotgun sequence.
ACCESSION   DBNHOH010000271 DBNHOH010000000
VERSION     DBNHOH010000271.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_143233"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..300)
                     /locus_tag="AC16V7_04815"
     CDS             complement(25..300)
                     /locus_tag="AC16V7_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256475.1"
                     /translation="MNTARRWASPDNFSYCDKLMRAAGFEKDTNHLSGYAFGRVFPTG
                     WHNLLQERKHSCSVFVNGVGLLPAHQDSGANNLLYAELAIYEIGKHP"
     gene            complement(442..1047)
                     /locus_tag="AC16V7_04820"
     CDS             complement(442..1047)
                     /locus_tag="AC16V7_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR6256476.1"
                     /translation="MKGFSISRNPRRLGLKAFSLLLVLALLTASLPVVALAAPSAATC
                     ARNYTVQSGDTLSKISVTYDVSIAELATANNLKEPYTLYVGQQLCIPGSTTTTTTTSS
                     SSSSSSSSSSSDKFSITLENDKIVVKVSGLTKNSAFVIKGMKYDRGDTSWIKFGNLKT
                     DKKGNGSVTLKLPKHLREGSYLVFCVKNLLNDKVECQRIRK"
BASE COUNT          352 a          302 c          351 g          379 t
ORIGIN      
        1 gtaagcgttc cttggggggt gtgttcatgg gtgtttacct atctcataaa ttgccagttc
       61 tgcgtataac aaattgttag caccggaatc ctggtgcgct ggcagcagac ccactccatt
      121 gacaaaaacc gagcaagagt gttttctctc ctgtaacagg ttgtgccaac ctgtcgggaa
      181 gacgcgccca aaggcatagc cggatagatg attggtatct ttctcgaatc cagctgcccg
      241 catcaatttg tcgcagtatg agaaattatc gggagatgcc caacgccggg cggtgttcaa
      301 agccttctac ttggcgcaat tcaagattta tcgagcgaac cttcatcgga ctccaaaaaa
      361 ctgtgggatt tccaggaaga tattacccgg aaatcccact tattcaagag agaatgtaat
      421 ataggggaat agctcgctaa gctacttacg gatcctctgg cactcgacct tgtcgttcag
      481 gaggttcttt acgcaaaaca ccaggtagct tccctctctt aggtgcttgg gtagcttgag
      541 cgtaaccgag ccgttaccct ttttgtccgt tttgagattg ccgaacttaa tccaggaggt
      601 atcgcctcgg tcgtatttca tgcccttaat tacgaatgca gagttcttcg tgaggccaga
      661 aacctttacg acgatcttgt cattttccag ggtgatgctg aatttatcgg acgagctgga
      721 agagctgctg gaagagctgc tggaagaggt ggtcgtagtt gtggtcgtgc tcccggggat
      781 gcatagttgc tgcccaacgt aaagggtgta gggctctttg aggttattgg cagtcgccag
      841 ctcggcgatg ctaacatcat aggtgactga gatcttgctc agcgtatctc cagattggac
      901 ggtgtaattc ctggcacaag ttgcagcaga cggggctgcc agggcgacaa ctggcaggga
      961 agctgtcaga agcgccagca ccaggagcag gctgaatgcc ttcaggccca gcctacgggg
     1021 attgcgagaa attgaaaagc ctttcattat gtatctcctt ttcagactct gacgatttta
     1081 ccacaataaa gttccgcttt tcacatctcg attttaggat tcgcttaagg aaattattag
     1141 agttttggca agaggcagcg attaaatacc aattatataa atctaatatt agatatttcg
     1201 gcgtatatat atctaagatg agatctagat ggtaaaatta gtcatttaga atgaggttag
     1261 tgtaatttaa tggctgaaca cagcattttt ggataccctt cacaactcga cacgttaatc
     1321 caggtcaata tacgggattt cttagtcgcc tttggtctgg agaaacttcg gcgcgggcga
     1381 aaag
//
LOCUS       DBNHOH010000272         2286 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1233561, whole genome shotgun sequence.
ACCESSION   DBNHOH010000272 DBNHOH010000000
VERSION     DBNHOH010000272.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1233561"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(385..579)
                     /locus_tag="AC16V7_04825"
     CDS             complement(385..579)
                     /locus_tag="AC16V7_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIR6256477.1"
                     /translation="MMTTRQALRRSRSNRVFMGVCGGLAEFFGISAFWFRLAFIIALI
                     PGGVPGILIYLLLWLIIPSE"
     gene            complement(637..1281)
                     /locus_tag="AC16V7_04830"
     CDS             complement(637..1281)
                     /locus_tag="AC16V7_04830"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIR6256478.1"
                     /translation="MTIYNDQISQYTTNLFAIHDKGLQRAWDETPRKGLPAISIKPEE
                     GRFLQFLTRACGAKKALEIGTLGGYSGIWIARGLVPGGKLITLELESRHAEIAREHFE
                     FAGVGESIEIRLGDAHHALEQLSSEAPFDFVFIDAEKTGYPKYLAWALEHTRVGGIIA
                     AHNAYRKGSVAGLAEPDAYTETMREFNISIATNPRLMSTIYPAGDGTLVSVKVA"
     gene            complement(1301..2218)
                     /locus_tag="AC16V7_04835"
     CDS             complement(1301..2218)
                     /locus_tag="AC16V7_04835"
                     /EC_number="3.5.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000118 - histone deacetylase complex
                     [Evidence IEA];
                     GO_function: GO:0004407 - histone deacetylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HIR6256479.1"
                     /translation="MKVFYSDRFAVPLPEGHRFPMPKYTLLRQRAFESGLLHPDEFIE
                     AEPASDIQLLRVHTAEYLEKVKVGLLSEREIRRIGLPWSPQLFERSRRSVGCTIEACR
                     VALQEGLAANLAGGTHHAYPDHGEGFCVFNDVAVAVRAMQAEDHASRMMILDCDVHQG
                     NGTAAIFAGDPTVFTFSIHGEKNFPYHKERSDLDIAMPDGTSDQVYLEALQDGLRRCL
                     EAFQADLAIYLAGADPYIHDRLGRLSLSKTGLVERDRTVLAACHQFGLPVAITMAGGY
                     AKDIHDVADIHMNTLIIAQQFFQNGKQSN"
BASE COUNT          566 a          577 c          578 g          565 t
ORIGIN      
        1 gaaattctgt gcgataaatc aagctatatc gtaatctatg tcgcctttgt acctcctttt
       61 caaggggcat tctgaaattt attttatcta tttaaccaag ttgggatgaa aacttaaata
      121 ctaaagagag caatgaaatt gctcaaagtc gaatttatca atttggactt tttgacaata
      181 gcagaaaaat aaacttgatt caagtgacaa acgtcatgtc acatttcctc aatgaggaaa
      241 aaggttgttc cgaaaataaa aggggtttgc gtggacgcat ttcagctgcc acacaaaccc
      301 aatcctgcaa ttaagcaaat acacatctct aaattggttt ggttatatga gatctcctca
      361 ccttatttcg agatactttc aagattattc gctggggatg atcagccaca gcaagaggta
      421 gatcaaaatt cccgggacgc cgccagggat aagggcgatg ataaacgcaa gccggaacca
      481 gaatgcgctt atcccgaaga actccgccag cccaccacac acgcccataa aaacacgatt
      541 gctgcgcgaa cgacgtaatg cttgtcgagt agtcatcata ttacctcctg taaaccttac
      601 atatccattt acgcaggtga agaataaaag ttgcactcag gcaaccttga ccgagaccag
      661 tgtgccgtct ccggctggat agatggtact catcaagcgt gggttagtgg cgatgctgat
      721 attaaactcg cgcatagttt cagtgtacgc gtccggttca gcaagcccag ctacacttcc
      781 ctttctgtat gcgttgtggg cggctatgat gcctcctacc cgtgtgtgct ccaatgccca
      841 ggctaagtat tttggataac cagttttctc cgcatcgata aagacaaaat cgaatggtgc
      901 ctctgacgac aattgctcca gggcatggtg ggcgtcgcct aaacgtattt cgatcgattc
      961 gccgaccccc gcgaattcga agtgctcgcg ggctatttcg gcatggcggg attccaactc
     1021 gagcgtgatc agtttcccgc cagggacaag gccgcgagcg atccaaatgc cgctgtaacc
     1081 gcctagcgtg ccaatttcca gggctttttt tgcgccgcag gcccgcgtca ggaattgcag
     1141 gaaccgcccc tcctctggct tgatgctgat ggcaggcagc cctttacgag gggtctcatc
     1201 ccaagcgcgc tggaggcctt tgtcatggat ggcgaacagg ttggttgtgt attggctgat
     1261 ctggtcgtta tagatggtca tgtaaggtta ctccataata ttaattggat tgcttcccat
     1321 tctgaaagaa ctgttgggca attatgagtg tattcatgtg gatatccgcg acatcatgaa
     1381 tatccttggc atatccgcct gccatggtga tcgctaccgg caacccaaat tggtggcagg
     1441 cggcgagaac cgtgcggtca cgctctacaa gaccggtttt gctcagggag agacgcccca
     1501 ggcggtcatg gatgtatggg tccgcgccgg cgaggtaaat cgccaaatct gcctggaagg
     1561 cttctaaaca gcgtcgcaac ccatcttgca aggcttccaa atagacttga tcactggttc
     1621 catccggcat ggcaatatcc aggtcggagc gttctttgtg atacgggaaa tttttctcac
     1681 catgaatgga aaaggtgaaa accgtgggat cgccagcgaa gatagcagcc gttccgttgc
     1741 cctggtggac atcgcaatcc aggatcatca tgcggctggc atgatcctct gcttgcatgg
     1801 cgcgcaccgc cacggcgaca tcgttgaaaa cgcaaaagcc ctcaccgtgg tccggatagg
     1861 catggtgagt gccccctgcc aggttggcag ccaggccttc ctgtaatgct acccggcaag
     1921 cctcgatggt gcagcctacg ctgcgccgcg agcgctcaaa taattgcgga gaccatggca
     1981 gaccaattcg gcgaatttcc ctttcgctca gcaacccgac tttgactttt tccaggtact
     2041 cagccgtgtg cacgcgcagg agttggatat ctgaggctgg ttcggcttct ataaattcgt
     2101 ctggatgcag caaacctgac tcaaacgctc gttgccgcaa cagagtgtac ttgggcatgg
     2161 ggaagcggtg cccctccggc aagggaactg cgaatcggtc tgagtaaaag accttcataa
     2221 tcgctattgt atcgcgatta atatggcgga ttatttattt cgcagggcaa ttgcatatgc
     2281 cctgag
//
LOCUS       DBNHOH010000273         1308 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1617884, whole genome shotgun sequence.
ACCESSION   DBNHOH010000273 DBNHOH010000000
VERSION     DBNHOH010000273.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1617884"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="AC16V7_04840"
     CDS             complement(<1..267)
                     /locus_tag="AC16V7_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR6256480.1"
                     /translation="MKGIILAGGRGTRLYPLTIGISKQLLPVYDKPMVYYPLSMLMLA
                     GIREILVISTPEALPAFKALLRDGSQWGLQFSYAEQSEPRGLADA"
     gene            complement(264..>1308)
                     /gene="rfbB"
                     /locus_tag="AC16V7_04845"
     CDS             complement(264..>1308)
                     /gene="rfbB"
                     /locus_tag="AC16V7_04845"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HIR6256481.1"
                     /translation="SNFVRYLLANEPDIQVVNLDALTYAGSTENLKELPDQERHTFVH
                     GNICDRGLVDDLLRRHQIDTIVHFAAESHVDRSILGPEQFVQTNVIGTFTLLEAARQL
                     WLEEKAIAREAVRFHHVSTDEVFGALTPAEPAWDESTPYDPHSPYSATKAGSDHLVRA
                     YQHTYQLPMTITNCSNNYGPNQFPEKLIPLMILNALDGRPLPVYGDGEQIRDWLYVED
                     HCEAIWQVLQHGTLGETYNIGGGNQPNNLTVVRTLCKILDKCLPESPNTPHDKLIQFV
                     PDRPGHDRRYAMDITKTNRELGWQPRHSLADGLLKTVQWYLSHVDWIDAIRKQQDFQG
                     WLARNYSKRGGNL"
BASE COUNT          291 a          330 c          370 g          317 t
ORIGIN      
        1 tgcgtccgcc agcccgcgcg gttcggattg ctcggcgtaa gagaattgca gaccccattg
       61 agatccatcc cggagcaggg ctttaaacgc gggtagcgcc tcgggcgtgc tgataaccaa
      121 aatttcgcgg atgcccgcca acattaacat ggataacgga taatacacca taggcttatc
      181 gtagaccggt agtaattgct tgctgatgcc aatggtgagc gggtacaggc gggtgccgcg
      241 cccgccggca aggataatgc ccttcataaa ttccctcctc gcttggagta attacgtgcc
      301 agccaaccct gaaaatcttg ctgtttgcga atagcgtcga tccaatccac gtgactcagg
      361 taccattgta cggttttcaa cagaccatcc gccagcgaat ggcgcggttg ccagcccaac
      421 tcgcggttag tcttggtaat gtccatggca tagcgccggt cgtgtccggg gcggtcgggc
      481 acgaactgaa ttagcttatc gtgtggagtg ttgggtgatt ccggtaggca tttgtccagg
      541 atcttacaca aggtcctcac cacggtcaag ttgttgggtt ggttacctcc accgatgttg
      601 tatgtctctc ccagggtgcc gtgttgcagc acctgccaaa tggcctcaca gtggtcttcc
      661 acatatagcc aatcgcgaat ttgttcgccg tcaccatata ccggcagggg acgaccgtcc
      721 aaggcattca gaatcatcaa tgggatcagt ttttcgggaa attggttggg tccgtagttg
      781 tttgagcagt tggtaatggt catcggcagt tgataagtat gttggtaggc acgaaccagg
      841 tgatcgctgc cggcctttgt tgccgagtac ggagagtgag gatcataagg agtggattca
      901 tcccaggctg gttctgcggg tgtcagggcg ccgaacacct cgtcagtaga gacgtgatgg
      961 aagcgtacgg cttcccttgc aatagctttt tcctcaagcc aaagctggcg cgcggcctct
     1021 aaaagcgtga atgtaccaat cacgttggtc tggacaaact gctcaggtcc cagtatggag
     1081 cggtccacat gcgattcagc agcgaagtgc acgatggtgt cgatctggtg acggcgcaac
     1141 aggtcatcca ctaaacctcg atcacaaata ttcccatgga caaaggtgtg ccgctcttga
     1201 tcaggcagtt ccttgagatt ttctgtgctg ccagcgtagg ttagcgcgtc aagattgaca
     1261 acttgaatgt ccggctcatt tgccaataag taacggacga agtttgag
//
LOCUS       DBNHOH010000274         2979 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_528392, whole genome shotgun sequence.
ACCESSION   DBNHOH010000274 DBNHOH010000000
VERSION     DBNHOH010000274.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_528392"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1239)
                     /locus_tag="AC16V7_04850"
     CDS             complement(124..1239)
                     /locus_tag="AC16V7_04850"
                     /inference="COORDINATES: protein motif:HMM:NF021539.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2270 domain-containing protein"
                     /protein_id="HIR6256482.1"
                     /translation="MYDSSSTSDVHPENDSQDKQTQSSQESDAVWTFRGYRLKSSEFT
                     TAMVHFFRAEISRANVWRTRLDATTNWAVVTTGATITIAFNPSGYHGVIILNTLLITL
                     FLFIEARRYRYYELWSYRVRLMETDFFASMLVPPFHPAPDWAEALAENLLHPDYPISM
                     WEAFGRRFRRNYMWIYIILAVAWVAKLWLLTPGPMTSWADFLAAASIGSLSGLIVLLI
                     GVVFNAALFLIGVLTTTLHKATGEVLPRYIQDIKILGPATATGQDTESRRAWFRQSRR
                     RQQLLAFVVTDQAQIVSQEILKEMARGVTSLEGVGMYTGKRHSVLMVALTTTEVAQLK
                     ALVSKTDANAFVIVSPAQEVLGRGFIPLKTKETEREN"
     gene            1325..1421
                     /locus_tag="AC16V7_04855"
     tRNA            1325..1421
                     /locus_tag="AC16V7_04855"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1359..1361,aa:Sec,seq:tca)
     gene            1483..2700
                     /locus_tag="AC16V7_04860"
     CDS             1483..2700
                     /locus_tag="AC16V7_04860"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR6256483.1"
                     /translation="MYPLEFDNLTSIVARVLQIEDITRGDPKKDFIARYRGQLYSEDT
                     AAAYDQLASALRPLEVTPYFRKEQESHVVILALGVNRPKPSKSWVNLVLFLLTVISVF
                     LAGTYYTLLGTYDGPMNPTFRSLLPYVLQSTGGGAAFAASLLAILLAHEFGHYLAGRY
                     HRTHVTLPYFIPFPFSAFGTLGAFIQLKEPPKNRRILLDIGIAGPLAGLVVAIPVLIL
                     GLSLSQVNPIPAQLPPGQGLQIEGNSVIYLLTKYLVFGKLLPEPGNYGNLPPWMYWVR
                     YFFTGRPLPLGGLDVLLHPVAWAGWAGLLVTALNLIPAGQLDGGHVLYVLLGKKSSFL
                     RPIILGALVLLGLVWPGWWLWAFLIFMLGRFHAEPLDQITSLDPPRQAIAILGLLIFF
                     LVFTPVPLLTVGL"
BASE COUNT          685 a          801 c          750 g          743 t
ORIGIN      
        1 ttgccgatct tctccaggct ctctactgaa cccggcttaa gcgccggaca atctggtgtg
       61 tctcattcat acattaacat ccgatgtagg tatcgcatta taccaagagg gcataggctg
      121 aaatcaattc tctcgttccg tttctttggt cttaagcggt ataaagcccc gacccagtac
      181 ctcttgggct ggggaaacga tcacaaaggc gttcgcatcc gttttactca ctagagcttt
      241 cagctgagct acttcagtgg tggttaaggc aaccatcaga acagaatgtc tctttccggt
      301 gtacataccc accccttcca acgaggtcac accccgtgcc atctccttta gaatttcctg
      361 acttacaatc tgagcttgat ctgtaacgac aaatgccagt agttgctggc gcctgcggga
      421 ttggcgaaac caggcacgcc ggctttccgt atcttgccct gtggcagtag ctggaccaag
      481 aattttgata tcctggatat agcgcggcag cacctcccca gtagccttgt gcaaggtggt
      541 ggtaagcacg ccaatcagga acaaggcggc attaaaaacc accccaataa gcaacacgat
      601 caagcctgaa agggagccga ttgaggctgc ggctaaaaaa tcagcccagg atgtcatcgg
      661 gccaggtgtt aagagccaaa gtttggccac ccatgcaacc gccaggataa tgtaaatcca
      721 catatagttt cgccggaacc gcctaccaaa tgcttcccac atagaaatcg ggtaatcagg
      781 gtgtagcaag ttttctgcca aagcctctgc ccaatcgggt gcaggatgga atggtggcac
      841 gagcatagag gcaaagaaat cggtctccat gaggcggacg cggtagctcc ataactcata
      901 ataacggtaa cgccgggctt caatgaaaag aaaaagagtg atcagcagcg tatttaatat
      961 gatcacccca tgatatccac tggggttaaa tgcaatggtg atagtcgcgc cagtggtcac
     1021 cactgcccaa ttggttgtag catccagccg cgtccgccac acgttggcgc gcgaaatctc
     1081 ggcgcgaaag aagtgcacca tggcggtggt aaattcgctt gacttcaagc gatacccacg
     1141 gaaagtccat acggcatcgg attcttggga gctctgcgtt tgcttgtctt gggaatcatt
     1201 ctctgggtga acatcggagg tggagctgga atcatacata gccgtattat atctatatta
     1261 atctgatagg acgttaattt atttaaatat ctaatctgat ataatccgcg tgtcccgttc
     1321 ctgggggagt ggttgggtcc cggtgggctc cccggtcttc aaaaccggtg gcgggtcgcg
     1381 ttgcgggccg cggtgggttc gactcccatc cactcccgcc atattattat ctgatgatta
     1441 agtttgagta tagagaaccg attactcgat tgatgatgtt atatgtatcc tcttgaattc
     1501 gataacctga cctcaattgt cgcgcgagtg ttgcagattg aagatattac gcgcggcgac
     1561 ccgaaaaaag acttcatcgc ccgctaccga gggcagctat atagcgagga cactgcagcc
     1621 gcgtacgacc agctggcctc agccctgcgc ccactcgaag tgacccctta tttccgcaaa
     1681 gaacaagaga gccatgtggt tattctggca ttgggagtaa atcgacccaa accttcaaaa
     1741 tcttgggtca atttggtatt gttcctgctg acggtgatct ccgtctttct ggctggaacg
     1801 tattacacac tgctcggcac ctacgatggc ccgatgaatc ccaccttccg cagcttgtta
     1861 ccttatgtgc tgcaaagtac ggggggaggc gcggcgtttg ccgccagctt gctggctatc
     1921 ctcctggcgc atgagttcgg gcattacctg gcagggcgct atcaccgcac acatgtcacc
     1981 ctgccgtatt ttatcccctt ccccttcagc gctttcggaa cgctgggtgc cttcatccag
     2041 ctaaaagaac cgcccaagaa ccggcgaatc ttgctggata tcggcatcgc cggcccactc
     2101 gcaggtctgg tcgtggccat cccagtgctg atactgggac tttccctctc gcaagttaac
     2161 cccatccctg ctcaattgcc acccgggcag ggcttgcaga tcgaaggaaa ctcggtaatt
     2221 tatctgctca ccaagtacct ggtctttggc aaactcctgc cggaaccagg caattatggc
     2281 aacctgccgc cttggatgta ctgggtgcgc tacttcttca cagggcgccc actgccactt
     2341 ggagggctgg atgttctgct tcatcccgta gcctgggccg gttgggcagg cttgctggta
     2401 acagcgctca acctgattcc agcaggtcag ctcgatggtg gtcatgtgct atatgtgtta
     2461 ttgggaaaga aatccagttt ccttcgaccg atcatcctgg gtgccttggt gctgttgggg
     2521 ctcgtttggc ctggatggtg gctgtgggca ttcctgattt ttatgctggg tcgcttccat
     2581 gccgagccgc ttgaccaaat cacctcttta gacccacctc gccaggcaat cgctattcta
     2641 ggtctgctga ttttcttcct ggtcttcacg ccggtgccat tgctgaccgt cggattgtag
     2701 taacccgcct cccgcgccag gaaaagggat ttgtctgatt cattggggtt tgtatgctga
     2761 gaccatacaa accccaatgt ctgtattaca cgcctcaatg cgcgccaggc aagattagcg
     2821 aggggatgtt ttgctcaaat tgcaatggac aacatacaat tttgttagga cctccccgct
     2881 cggagcaaat aaaataactt caattgacgc gctcttgtga ccatgctaga atgactccgt
     2941 tcggaaggat aaaaatccat ccgccatagc caaccttca
//
LOCUS       DBNHOH010000275        10593 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1553738, whole genome shotgun sequence.
ACCESSION   DBNHOH010000275 DBNHOH010000000
VERSION     DBNHOH010000275.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10593)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10593)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10593
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1553738"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /gene="rarD"
                     /locus_tag="AC16V7_04865"
     CDS             complement(<1..757)
                     /gene="rarD"
                     /locus_tag="AC16V7_04865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HIR6256484.1"
                     /translation="MSNKGILYGIGAYLMWGFFPIYFKAIHTVPALQIMFHRVVWCFI
                     FLAILILLKKEWGKLKAQVTKPKTLVIYTLAASLLAVNWLVYIYGVNSDQIVETSLGY
                     FINPLLNVALGVIVLREKLRPAQWVPIGLAAAGVTYLTMQHGALPWIALALAFSFGLY
                     GLVKKVSPLGSLYGLTLETAILFLPGLAYLLFAEAQGSGSFAHAGWQVTLLLALTGVV
                     TALPLLLFASAARSIPLYMVGILQFIAPTCQFLL"
     gene            1125..1541
                     /locus_tag="AC16V7_04870"
     CDS             1125..1541
                     /locus_tag="AC16V7_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256485.1"
                     /translation="MNKRFPHIMIVLTLVAVLAGALAFSTSTAKAENGTFNVSVYHDI
                     NGRNLGLSRELPVIATVSSTDGLYAVVPLKFKDRFSADLPAGTYTIMVSAVINGSLVE
                     LPSMSVGPVTIPADVDVRLIARLKSDGTPYIKVRVQ"
     gene            1762..2583
                     /locus_tag="AC16V7_04875"
     CDS             1762..2583
                     /locus_tag="AC16V7_04875"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6256486.1"
                     /translation="MLNILKDLSAPSLARSVKANLYAFFHILGRSSKSEFYTHPKLVR
                     WHTPIPHPWFQGILSAESAELDDDQLVRDMASYFKSRGVTSFTWWLEPNLRPDSWSSH
                     LSAHGFRYDKSTPGMAVHLAALPGSVPQPEKLVIRVVDDLEALEVWVRSFMLGYGLPE
                     AWSIDFFELLASLGLDLPIRHYLAYLDGEPVANSTLLLGAGVAGIYNVATIPEARGQG
                     IGAALTLFPLQQAAELGYHAGILQSSEMGFSVYRRLGFQHLCQMDHFFWKSDVDS"
     gene            2713..3678
                     /locus_tag="AC16V7_04880"
     CDS             2713..3678
                     /locus_tag="AC16V7_04880"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010698471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HIR6256487.1"
                     /translation="MTNAKPWKYRKVSIVGAGAVGSTFAYALAQSGLASEIAIIDRNQ
                     ELARGQVLDLLHGVPFFPSVEIHAGEQRDYADSQVIVITAGSKQSQGETRLDLLQRNA
                     SIIEAIVDDILHERSQAILVIVSNPVDVLTYVALKRSGWDKSRVIGSGTVLDSARFRY
                     ALSRHCDVDVRNVHAYILGEHGDSEVAAWSMTHIAGVPIDDYCPFCKRCGNWEEEKSR
                     IVEEVRNSAYHIIDYKGATFYAIGLALVSIVGAILRNQRSVLTVSNLLDGEYGMKDVC
                     LSVPCVLSERGIEHIIEAKLMPHEQEALSNSGSILRSALLELGHG"
     gene            3700..5790
                     /locus_tag="AC16V7_04885"
     CDS             3700..5790
                     /locus_tag="AC16V7_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012861.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HIR6256488.1"
                     /translation="MEDARTQAKRVLDRIVLSETTVSLGTAILVGAGTGIGAVLFIRL
                     IELVQKIFFEGSELALGSLGRGLILLIPILGGLLAGPIIAFFASEAKGHGVPEVMQAI
                     ALRGGRIRPRVVVAKILASALCIGSGGSAGREGPIVQVGAALGSSIGQGLKLSSDRIR
                     NLVACGAAAGIAATFNAPIAGVIFAMEVILGELHLGDLGNVVISAVTASTLARVMLGE
                     NPAFAIPSYGMRTPWEVLLYVLLGALAAFVAVGFIKILYWFEDLFDNWRFPQAVKPAV
                     GGLLLGSLALIYPLVLARGGVPAQEARTGLPLIANIPHVFGSGFSVIEDALLGRSSFL
                     LLLALIFLKPLATSFTLGSGNSGGVFAPALFTGAALGGAFGLLVERLFPGATAGPGAF
                     ATVGMAAVFAGAARAPFSAILIVFEMTNDYRLIVPLMAAVIASLILAERLHKESIYTL
                     KLTRRGIRLRRGRDVDVMENLTVSEVMRPAPEPVPALTPLGMVPALLEGVRMHGLPVV
                     DGVGNLCGVITVQDIEAAVEQDEHNIDLPVTRFCSQRLVVAYPDETVNQALARMSSRD
                     IGRLPVVDPAQTRRLLGWISRSDIIRAYERALTRRVAIQHQLTHARLGAVSGVNVFEF
                     VVASGSAVDGKSLSEIAWPEDSLVASVHRGQRLIIPHGNTVLQAGDRLSIVAHVHDEK
                     LLQALVSAASSSNN"
     gene            5898..6671
                     /locus_tag="AC16V7_04890"
     CDS             5898..6671
                     /locus_tag="AC16V7_04890"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR6256489.1"
                     /translation="MTLSLTDLEFLARQAGDILRAKYGQRQQVDHKGIIDLVTEADRQ
                     SEAFLLAQIRRRFPDHRILAEESGGQAGEDCCLWYIDPLDGTVNYAHGVPIFSVSLAF
                     QEMGRMRFGVVYDPMRDECFSAEAGSGAWLNGEPIRVSDTPDLDNSLLVTGFPYDIRE
                     NPDNNLDHYTRFSLLSQAVRRLGSAALDLSYVACGRFDGFWELRLSPWDVAAGALIAQ
                     EAGAVVTNIHGGTDYLSPQQSILAANPHIHAVMLAQFEK"
     gene            complement(6679..7479)
                     /gene="surE"
                     /locus_tag="AC16V7_04895"
     CDS             complement(6679..7479)
                     /gene="surE"
                     /locus_tag="AC16V7_04895"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HIR6256490.1"
                     /translation="MEKPQILLTNDDGIRSPGLWAAAKALSEIGFVHVAAPREQYSGA
                     GRSMPNHSDGVIQVEQVHVNGDAWIVHAVGGTPAQAVQHAMFEILPQRPSLIVSGINY
                     GENLGSGVTISGTVGAALEGAAFGIPSLAISLETSVNHHLSYSDDVDFSIAGHFTALF
                     GRKLLEHRLPPDVHVLKVDVPADATPATPWEITRLSPTNYFTAIPTQRRSWSEPGKIS
                     YRVSDGLQDEPPGTDIYALHIQRHVAVTPLSLDLTSRVDLMILKDSLE"
     gene            complement(7445..7966)
                     /locus_tag="AC16V7_04900"
     CDS             complement(7445..7966)
                     /locus_tag="AC16V7_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HIR6256491.1"
                     /translation="MEKKPSPPPDTLNVIGVLTRREIEARLLAPLLEAFSEEFGRQRA
                     LEITRQTIIRIAREQGTSLAQSMGGNSLAHFADTLEAWKKDDAMQIEVLEQSEGRFAF
                     DVTRCRYAEMYNALGVPELGGLLSCNRDHALIEGFNPQVQLTRTQTIMEGAPSCDFRY
                     TIYGKTPDTPNQR"
     gene            complement(7998..8432)
                     /locus_tag="AC16V7_04905"
     CDS             complement(7998..8432)
                     /locus_tag="AC16V7_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR6256492.1"
                     /translation="MITVKQILRSKGHAAWTISPDASVLEALQLMEQKDIGALVVVEG
                     KKLVGMFSERDYARKFIHGMGSTKDIQVRELMSAPVDTVRPGMSIGACMALMTERRFR
                     HLPVVEGENLVGMISIGDVVKAIISEQQKVIDELGNFINGKR"
     gene            complement(8434..>10593)
                     /locus_tag="AC16V7_04910"
     CDS             complement(8434..>10593)
                     /locus_tag="AC16V7_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6256493.1"
                     /translation="HFRVYTPGSDAGIPVSILASLKCPPISWDGNRELLREKISGTVT
                     ALLGLVGLDDIDPVRSREHILLSNIFEAAWSQAKDLDLGELILQTQTPPFAKLGVFDV
                     NTFFPEKDRFELAMLLNNILAAPAFQIWIEGEPLEIQDLLYTSDGRPRHSVFYIAHLS
                     DSERMFFVTLLYSAMETWMRSQPGTTSLRTILYFDEIFGYMPPVGNPPSKHPMLRMLK
                     QGRAFGVGQVLVTQNPVDMDYKALSNAGTWFIGKLQTEQDKERLLDGLESAIASGLNR
                     AEYDRLISTLGKRIFLLHNVHAKGAQLFQTRWAMNFLAGPLTRAQIPQLNQLASGEPG
                     LAVKDQQTQALVTDGVSDQTEINSVQAGPQPDSIPTPAPVSSPAPVHSPQPMAASTRP
                     TLPAGLKEYFLPYNLTFSQAFKAAGRPLSKEAIIQGLVYRPVILAQVEVRFLNRKYNL
                     DTILRKTALVAEPDRRGLVRWEEYIAPGVDAASLDAAPDPQARYAVPEAPFTDPKTMT
                     MLEKDFLDWVYRTIQVKVRANEQLKLYAGPQISSAEFRTQCAEAARQGRDAEIRKISS
                     AYDAKIATIQTKLSREERELSEDQAELSQRKMEELGTHAENIFGIFGGRKSSRRLSSS
                     LSKRRMTSKAKADVDESLDAITEYKKQIADLEKDKAAALQEVNDHWSQIANQTEEINI
                     TPLKKDILLDLFGVAWMPYHLIRIGEELLELPAYAEI"
BASE COUNT         2347 a         2673 c         2950 g         2623 t
ORIGIN      
        1 ccagcaggaa ctggcaggta ggggcgatga actgcaagat gcccaccatg tagagcggaa
       61 tcgaccgggc tgcggaggcg aacaagagca gcggtagcgc agtgacaacc ccggtcagcg
      121 ccagcaagag cgtcacctgc cagccggcgt gggcgaagga tccactgcct tgggcttctg
      181 caaatagcag gtaagccagg ccgggcagga agagtatggc tgtttccaac gtcaggccat
      241 acagtgaccc taagggggag actttcttca ccaggccata gagaccaaag ctgaaggcca
      301 gcgccagggc gatccacggt agggctccgt gctgcatggt gagataggtc accccggcag
      361 ccgctagccc gatgggcacc cactgcgccg gacgcagctt ttcacgcagg acgatgaccc
      421 ctaatgccac gttcaacaag ggattgataa agtaacccag gctggtctct acaatctggt
      481 cgctgttgac tccgtagatg tacaccagcc agttaaccgc cagcaggctg gctgccaggg
      541 tatagatgac cagggttttc ggcttggtga cctgcgcttt cagtttcccc cattcttttt
      601 tgagcaggat gagaattgcc aggaatataa agcaccacac cacccgatgg aacatgatct
      661 gtagcgccgg cactgtgtgg atggctttaa agtaaatggg gaagaacccc cacatcaggt
      721 atgcgccgat accatataag atacccttgt tggacatcct aacctctggg aatagagtaa
      781 ataacaggta tcaatgatac caccttcttt gcatgcgatc atggatggtt cgagatttat
      841 tagggcaatc gcatttgggc gaattcccac cactgaggag agaaaatggg gtgttttggt
      901 gggggaatgc ccccaccaaa acaccccaaa cactttttta cgggattgcg catgcccctc
      961 agggcatatg caattgccct gcgagattta ttatctaagg agattgattc attttcaagc
     1021 tttaatggaa tactaagctt taagccttga catattgtat aagtattgta taatatatat
     1081 atcaatacct atacaatatt tatacattat taaaggagct tacgatgaac aaacgctttc
     1141 cccacattat gattgtccta actttggtag ctgtcttggc tggtgctctg gcctttagca
     1201 cgtcgacagc caaagctgaa aatggcacct tcaatgtctc tgtgtaccat gatatcaatg
     1261 gcaggaacct gggtctctcg agggaattac ctgtaatcgc cacggtttcc tccaccgacg
     1321 gtttatatgc tgtagtccca ttgaagttca aagatcgttt ctctgctgat ttgcccgctg
     1381 gcacctacac gattatggtc tctgcggtca taaacggtag cctggttgag ctgcccagca
     1441 tgagcgttgg cccggttacc atccctgccg atgtagatgt gcgcctgatt gccaggctta
     1501 aatcagatgg caccccctat atcaaagtac gtgtgcaata atcatcaagt aatacttttg
     1561 agattgttgt ccaaaacata actttgcaat cgaataggtg ttgtacccaa gccccggagt
     1621 tcaatccggg gcatggtctt taatttgatt atgggtgaaa atatttcttc catctctaga
     1681 atgggagtaa aaatttatga gtataattcg gagaccgacc caccgagatt agaatgttgt
     1741 aactcgagat cgaatattgc catgttaaat atactgaaag atctctccgc cccgagcctc
     1801 gccagatcag tcaaagccaa cctgtacgcc ttcttccata tacttggacg ttcctcaaaa
     1861 tctgagttct atacacatcc caaattggtg cgttggcata ccccgattcc gcacccttgg
     1921 tttcagggca tcctcagcgc agagtctgct gaactggacg acgaccagct ggtccgcgat
     1981 atggcatcct atttcaaatc acgcggcgtg acgagtttta cctggtggtt ggagcccaat
     2041 ctaaggccgg atagctggtc tagccacctg agcgcacatg gctttcgcta cgataaaagc
     2101 actcctggca tggctgtgca cctggctgct ttaccaggct ccgttccaca gcccgagaaa
     2161 ctcgtaatcc gggtagttga tgatctggag gccttagagg tgtgggtacg tagctttatg
     2221 cttggctacg ggttgcccga agcgtggtcg atagactttt ttgaactgct tgccagcctg
     2281 ggactcgatc ttccaattcg gcattacctt gcctatttgg atggcgaacc cgtggcaaac
     2341 tccacactct tgctcggagc tggtgtggca ggcatctata atgttgccac gattcctgaa
     2401 gcaagggggc aagggatcgg cgcagctctg acgttgttcc cgttgcagca agccgctgaa
     2461 ttaggctatc acgctgggat cctgcaatcg tccgagatgg ggttcagcgt atatcggcgt
     2521 ttgggttttc aacatctctg ccagatggat cattttttct ggaagtctga tgtggatagc
     2581 tgacaggaag tggttgataa taaagacagg atttccacaa cgggagatgg gatgaggaag
     2641 cttggggagg gatccctcca tcgatccatt cttcattaga ttgcgcaaat cctgagagag
     2701 aaggtatttc ttatgaccaa cgcaaagcct tggaagtatc gaaaggtttc tatagtcggt
     2761 gcaggcgccg tgggatccac gtttgcctat gccttagcac aaagcggttt agcctctgag
     2821 atcgctataa tagaccgtaa tcaagagtta gcgcgtggac aggtgcttga cctgctgcat
     2881 ggggtgccat ttttcccttc ggttgagatc catgcagggg agcaacgaga ttatgccgat
     2941 tcgcaagtga tcgtgattac cgcgggcagc aaacaaagcc agggggaaac gcgcctggac
     3001 ctgctccagc gcaatgccag catcattgaa gcaatcgttg atgatattct acatgagaga
     3061 tctcaggcaa tcctggtaat tgtcagcaat cccgtggatg tcctgaccta tgtggctttg
     3121 aaaagatcag gttgggataa aagcagggtc attggttcag gcactgtact ggatagcgct
     3181 cgctttcggt atgcactcag ccgccattgc gatgtggatg tgcgcaatgt tcatgcttac
     3241 attttaggcg agcacgggga tagtgaagta gcggcctggt cgatgacgca cattgcaggc
     3301 gtgccgatcg atgattattg cccgttctgt aagcgttgtg gaaattggga ggaggagaaa
     3361 tccaggatcg tcgaggaggt gcgcaattcc gcctaccata ttatcgatta taaaggggcc
     3421 actttttatg ctatcggatt agccttggtg agcattgtgg gggcgatctt acgcaaccag
     3481 cgcagtgtgc tcaccgtatc aaatcttctg gatggggagt atggcatgaa ggacgtgtgc
     3541 ttgagcgtcc cttgcgtgct ttcggagcga gggatcgagc acattattga agccaagctc
     3601 atgccacacg agcaagaggc gctatccaat tcgggatcta ttctccggag cgcattacta
     3661 gagcttgggc acggttaaat gcccttttgg agagaaataa tggaagatgc tcgtacgcag
     3721 gccaagagag tacttgatcg cattgttcta tctgaaacaa ctgtgtcgct cggaacagcc
     3781 atcctggttg gagcaggcac tgggatcggt gcggtcttat tcattcgact gatcgagctc
     3841 gtacaaaaaa tattcttcga aggcagcgag ctagcgcttg gctctctggg tcgaggactg
     3901 atcctgctta tacccatatt gggtggattg ctggctgggc cgattatcgc tttcttcgcc
     3961 agtgaggcca agggacatgg cgttcccgag gtcatgcagg caattgccct gcgcggcggg
     4021 cgcatccgac cccgagttgt agtggctaaa attcttgctt ccgcgttatg catagggagc
     4081 ggcggatctg ctggtcgcga gggtccaatt gtgcaagtcg gcgcggcgct gggctcctcg
     4141 attggtcagg gactgaaact atcgtctgat cgcatccgaa acctggtggc ttgtggggca
     4201 gcagctggga ttgcagccac cttcaacgcg ccaattgctg gggtgatctt tgccatggag
     4261 gtgatcctgg gtgagttgca tttaggggat ctgggtaatg tggtcatttc agcggtcacg
     4321 gcttccacgc tggcgcgcgt gatgttgggt gagaaccccg cctttgccat cccgagttac
     4381 ggtatgcgta cgccttggga ggtgcttctc tatgtgctgc tgggcgccct ggcagccttt
     4441 gttgcagttg gatttattaa aatcttgtat tggttcgaag atctttttga caactggcgt
     4501 tttccacagg cggttaaacc tgctgtgggt gggctcttgt taggtagtct ggcgctgatt
     4561 tacccattgg ttctcgctcg gggtggtgta ccagcccagg aagcacgcac cggtctgcct
     4621 ctaatcgcta atatccccca tgtttttggt tccggttttt cggtgatcga agatgccttg
     4681 ctgggaagaa gctcatttct gcttttactg gctttgatct tcttgaaacc tttggctaca
     4741 tcttttacct tgggatcagg taattctggt ggggtttttg cgcctgctct gtttacaggg
     4801 gctgccttgg gtggtgcgtt tggcctttta gttgagcggc tattccccgg tgccacagca
     4861 ggaccagggg ctttcgccac ggttggcatg gcagcggtct ttgctggagc ggcacgtgcc
     4921 cccttctcgg caatactgat cgtgttcgag atgaccaacg attatcgctt gattgtgccc
     4981 ttgatggcgg cggtgatcgc cagcctgatc ttagcggagc gcttgcacaa ggagtcgatc
     5041 tacaccctaa aactcacgcg gcgtggcatt cgcttgcggc gcggccggga tgtggacgtt
     5101 atggaaaacc tcaccgtctc cgaagtgatg cgccctgcgc cggaaccggt gcccgcatta
     5161 acccctctag gcatggtccc ggctctgctg gaaggggtgc gcatgcatgg tctgcctgtg
     5221 gttgatggcg ttgggaatct gtgtggtgtg atcacagtgc aggatatcga agctgctgtg
     5281 gaacaagacg agcataacat cgatctgccc gtgacccgtt tctgttctca gcggctggta
     5341 gtagcttacc ccgatgaaac cgtcaaccaa gccctggcac gcatgagcag ccgggatatt
     5401 ggtcgccttc cagtcgtcga ccctgctcaa acgcgtagac ttctcgggtg gatcagccgt
     5461 tcggatatca tacgggctta tgaacgagcg ctgacgcgac gcgtggcgat ccaacaccag
     5521 ctgacccatg ctcgtttggg agctgtctcc ggtgtcaatg ttttcgagtt tgtggtggca
     5581 tctggcagtg cggtggatgg gaaatcgctg tcggaaattg catggccgga ggatagcctg
     5641 gtggccagtg tacaccgtgg tcagcgtttg atcattccac atgggaatac agtgctccaa
     5701 gctggggacc ggttgtcgat tgtcgctcat gtgcatgatg aaaagttgtt acaggcatta
     5761 gtgagtgcag caagtagctc gaataattaa ggtaacaaaa taccatgaag gtgctacaca
     5821 ccgacctgcg ccaatggtga agaaatcctg cgcgcgctga aaaatatcta tcgtttatta
     5881 ggacaataca acagcttatg accctctcat taacagacct cgaattttta gcacgccagg
     5941 cgggtgacat cctgcgtgcc aagtacggtc agcggcaaca ggtcgaccac aagggaatca
     6001 tcgacctggt caccgaagcg gatcgccaat ccgaggcttt tctactggcg caaatccgta
     6061 ggcgctttcc tgatcaccgc atcctggctg aggagagcgg agggcaagct ggagaagact
     6121 gctgtctgtg gtatatagac ccgttagatg gcacggtcaa ttatgcccat ggcgtcccaa
     6181 ttttctctgt atcactggca ttccaggaga tggggaggat gcgcttcggc gttgtttacg
     6241 acccaatgcg ggatgagtgc ttcagtgcgg aggcggggag cggtgcctgg ctgaatgggg
     6301 aacccatccg cgtctcagac acacctgatt tggataacag cctgctggtc accggttttc
     6361 cctacgatat tcgcgaaaat ccggataaca acctggatca ctatacccgc ttttcactgc
     6421 tcagccaggc agttcgccgc ctgggttcgg ctgctctaga tttatcttat gtagcctgcg
     6481 ggcgtttcga tggcttttgg gaattacgcc tgagcccttg ggatgtggcg gctggtgcgc
     6541 tgattgcgca ggaggccgga gcggtggtta cgaacatcca tggagggacc gattacctct
     6601 ccccacaaca atccatctta gctgccaacc cccatattca cgctgtcatg ttggcgcagt
     6661 ttgaaaaata gggatttatt actccaggga gtccttcagt atcattaaat cgacgcgcga
     6721 agtcagatcc aggctgagag gcgttaccgc cacgtggcgc tggatatgca gggcgtaaat
     6781 gtccgtcccc gggggttcat cctgcaatcc atcggaaacc cggtagctaa tcttgccagg
     6841 ctcgctccag gagcggcgtt gtgtggggat ggctgtaaaa taattggtcg gggaaagtcg
     6901 cgtgatctcc catggagttg cgggtgtggc atcggctggc acatcgactt tgagaacgtg
     6961 cacatcgggc ggtagccgat gctccagcaa cttgcgcccg aagagggcgg taaagtgccc
     7021 cgcgattgaa aaatctacat catctgagta tgatagatgg tggttcacgg atgtctcgag
     7081 ggatatggcc agcgaaggga tgccgaaagc tgcgccctcc agagcagcgc ccactgtccc
     7141 ggagatcgtc acgccgctac cgaggttctc cccataattg atacctgaga caatcaagct
     7201 gggtcgttga ggcaagattt caaacattgc atgttgcaca gcctgtgctg gcgtaccacc
     7261 cactgcgtgc acaatccagg catctccatt gacatgtact tgctcaacct ggatcacccc
     7321 atcggaatgg ttgggcatgc tgcgaccagc cccggaatat tgctcacgcg gcgcggcgac
     7381 atgaacgaaa ccgatttcag aaagcgcctt tgccgctgcc cataatccgg gcgagcggat
     7441 gccatcatcg ttggttagga gtatctgggg tttttccata aatagtatag cggaaatcgc
     7501 atgaaggggc gccctccatg atggtctgcg tgcgcgtcag ctggacctgc gggttgaagc
     7561 cctcgatcaa ggcatgatcg cggttacagg agagcagtcc ccctaactct ggaacgccca
     7621 gggcgttgta catctcggca tagcggcagc gggttacgtc aaaagcaaat cgtccttcgc
     7681 tttgctctag cacctcgatc tgcatcgcat cgtccttctt ccaggcctcc aacgtgtcag
     7741 caaagtgcgc caaactgtta ccgcccatgc tttgggcgag actcgtacct tgctcacggg
     7801 cgatacggat gatggtctgg cgagtgatct ccagcgcgcg ctggcgccca aactcttcgg
     7861 aaaaagcctc tagcagtgga gccaagagac gcgcctcgat ctcgcgacga gtcagtacgc
     7921 ctatgacatt caaagtgtcg gggggcgggg atggtttctt ttccaagata actccattat
     7981 tttcaaacat taatagatca gcgcttacca ttgataaagt tacccagttc gtcaatcacc
     8041 ttttgctgtt cggagatgat tgctttgacc acatcaccaa tagagatcat gccgaccaaa
     8101 ttttcaccct cgacaacagg caggtggcgg aaacgccgtt cagtcatcag tgccatgcaa
     8161 gcgccgatgc tcatccctgg gcggacggtg tccaccggcg cgctcatcag ttcccgcacc
     8221 tggatatctt tggtcgaacc cattccatgg ataaacttgc gggcatagtc gcgttctgag
     8281 aacatgccga caagcttttt gccttcaaca actaccaacg cccctatgtc tttctgttcc
     8341 atcaattgca aggcttcaag gacgctggca tctggtgaga tcgtccaggc agcatggccc
     8401 ttagagcgca gaatttgttt gactgttatc atctcaaatt tcagcgtagg caggtaattc
     8461 gagtaattct tcgccaatac ggattagatg gtacggcatc catgctaccc caaacaggtc
     8521 cagcaagata tcttttttca ggggagtgat gtttatttct tctgtttgat tagcaatttg
     8581 actccagtga tcgttgacct cctgcaaggc agcggcttta tctttctcca gatctgcaat
     8641 ctgcttctta tattctgtga tggcgtccag cgattcgtca acatcggcct tagcttttga
     8701 cgtcatgcgt cgtttggaga gcgaactgga cagccgccgg gagctcttac gtcccccaaa
     8761 tatgccgaaa atgttttccg catgggtgcc aagctcttcc atctttcgct gtgagagctc
     8821 agcctgatct tcactcagct cgcgctcttc gcgcgacagt tttgtctgta tggttgcgat
     8881 cttcgcatca taggcgctgg agatcttgcg aatttcagca tctcgaccct gccgggcggc
     8941 ttcggcacat tgagtgcgga actccgcgga ggaaatctga ggccccgcat agagtttgag
     9001 ttgttcgttg gcgcgcactt ttacttggat ggtgcgatag acccaatcta ggaaatcttt
     9061 ttccagcata gtcattgttt tgggatccgt gaagggcgcc tctggcactg catacctggc
     9121 ttgcgggtcg ggagcagcat ccaggctagc ggcatccacc ccaggggcta tgtactcttc
     9181 ccaacgaact agcccgcgcc ggtctggctc tgccactagg gcggtcttgc gcagtatggt
     9241 atccaagttg tacttgcgat tcaagaagcg cacttccacc tgagccagga ttaccgggcg
     9301 gtaaactaaa ccctgaatga tggcctcctt tgatagcggg cggccagcgg ctttgaaagc
     9361 ttgtgaaaag gtcaggttat agggtaggaa gtattccttc aacccggctg gcagagtggg
     9421 acgagtcgaa gcagccatgg gttgaggtga gtggacaggc gcgggggagc tgacgggcgc
     9481 aggggtgggg atgctgtctg gttgcggccc agcttggacg gagtttatct ccgtttgatc
     9541 agacactccg tctgtcacga gcgcctgcgt ctgctgatcc ttaacagcta atcctggctc
     9601 gccactcgcc aattgattta attgtggtat ctgggcgcga gttagtggcc cagccaggaa
     9661 gttcatcgcc cagcgcgtct ggaataattg tgcccctttg gcatgcacgt tgtgtaacag
     9721 gaagatccgc ttgcccaagg ttgagatcag gcgatcgtat tcggcgcggt tgagaccgga
     9781 agcaatggca ctctccagtc cgtctagcaa gcgctccttg tcctgctcag tttgcagttt
     9841 gccaataaac catgtcccgg cattcgagag agctttgtaa tccatatcta ctggattttg
     9901 ggtgacgagc acctgtccca caccgaaggc gcgcccttgc ttcaacatac gcagcatggg
     9961 gtgcttggag ggagggttgc ccactggtgg catataacca aagatttcgt caaaatacaa
    10021 aattgtgcgt aatgacgtcg tccctggctg agaccgcatc caggtctcca tcgccgagta
    10081 gagcagtgtg acaaaaaaca tgcgttccga atcactaaga tgtgcaatat aaaacacact
    10141 atgacgtggt cgaccatcgg aggtgtaaag aaggtcctgg atctccaggg gctcaccttc
    10201 aatccaaatc tggaacgcag gagcggccaa gatgttattg agcaacatgg ctagctcaaa
    10261 acggtccttc tctgggaaga aggtgttcac atcgaatacg cctagcttgg cgaaaggtgg
    10321 tgtttgagtt tgcaggatga gttcgcctag gtcgagatct tttgcctggc tccaggcggc
    10381 ctcaaagata ttgcttagca ggatgtgttc ccgagagcgc actgggtcga tgtcgtccaa
    10441 gccaaccaaa cccagcagcg cagtgacggt gcctgagatc ttctcccgta agagttcgcg
    10501 atttccgtcc caggagatcg gcgggcactt cagcgaagcg agaatgctga ccggaattcc
    10561 ggcgtccgag ccaggagtat aaacgcggaa gtg
//
LOCUS       DBNHOH010000276         3819 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1169484, whole genome shotgun sequence.
ACCESSION   DBNHOH010000276 DBNHOH010000000
VERSION     DBNHOH010000276.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1169484"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..535)
                     /locus_tag="AC16V7_04915"
     CDS             complement(8..535)
                     /locus_tag="AC16V7_04915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 domain-containing protein"
                     /protein_id="HIR6256494.1"
                     /translation="MPTKEIDLRPVSHITTDAIGPPGKRIFYIQGWQEERTVTLIVEK
                     IQIQSLAVGLEQFLAEIYERFPNLTKASSEYVEENMHIHPPLDPLFRTGELGLGYDAE
                     NDLVVLIARELLTEDQDPEEASVVRYWCSRDQIRALCHWGMEVASRGRPLCPQCGQPM
                     DPEGHFCPKKNGHKH"
     gene            complement(577..1194)
                     /locus_tag="AC16V7_04920"
     CDS             complement(577..1194)
                     /locus_tag="AC16V7_04920"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR6256495.1"
                     /translation="MPTILLIRHGENDFVKKGIMPGRMPGIHLNDNGRAQAQAVAEKL
                     RQAPIKAIYTSPLERAAETAEYLAKALGQETIVRPGLMESDIGEWQGKSHKSLRRLKV
                     WRIVQSTPSLFRFPGGESFAECQARMVGEIETLRAQYEEKDIFVCVSHADPIKLAVAY
                     YLGLPLDQFQRLFIAPASITALQVGQMGSYLLALNYDLSFNLSKP"
     gene            complement(1199..2608)
                     /locus_tag="AC16V7_04925"
     CDS             complement(1199..2608)
                     /locus_tag="AC16V7_04925"
                     /inference="COORDINATES: protein motif:HMM:NF009881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6256496.1"
                     /translation="MDLFSHAMHERMKSEAPLAARMRPRILDEFVGQEEIVGPGRLLR
                     RAIESDRLFSSIILWGPPGSGKTTLAMVIANHTRMHFETLSAVLAGKAELKKVIEAAI
                     ERRKLHNRRTILFVDEVHRWNKAQQDALLPHVENGNITLIGATTENPYFEVIGALVSR
                     SRVFQLRPLEDEHVRRILEYALQDDERGYGAKHIKLDEDALEHLIRVAGGDARNALNA
                     LELAVESTPAGDQETIHITLQVAQESIQRRAVLYDKDGDAHYDTISAFIKSVRGSDPD
                     AALYWLAKMLYAGEDPRFILRRLIILAGEDIGLADPLGLVIANAAAQSFDYIGLPEGI
                     YPIVEATLYLATAPKSNTATSYFKAFQLIEDEGITAVPQHLQDANRDAKPLGHGQGYQ
                     YPHEDPEHFLPQQYLPHPLLGTYFYKPSSQGYESQVIDRLSRWRDAQRKALGITQTTE
                     LPEPSQDEIQEIKHKHKAG"
     gene            3061..3432
                     /locus_tag="AC16V7_04930"
     CDS             3061..3432
                     /locus_tag="AC16V7_04930"
                     /inference="COORDINATES: protein motif:HMM:NF014368.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIR6256497.1"
                     /translation="MGSSQESKTIRVLLVCAFGFSTALLEARITEAAKAAGVNLDIRG
                     DTVDGIPLIDFEKKIFDVILLAPQVRFMRKRIAQRVAPFNIVVQVLDPMAFGMMDGEL
                     LFRQILEALQAHEATELSSLS"
     gene            3599..>3819
                     /locus_tag="AC16V7_04935"
     CDS             3599..>3819
                     /locus_tag="AC16V7_04935"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6256498.1"
                     /translation="MAPDNTITLYKWILKEFTIAERGKAVQKIGRHLAPLVQAGDQVL
                     DLCCGTGPASFWFEERGAAVTAIDFAPYM"
BASE COUNT          828 a          970 c         1084 g          937 t
ORIGIN      
        1 agaaggatca atgtttatgc ccgtttttct tggggcagaa atgtccttcg gggtccatgg
       61 gttgcccgca ttgtgggcag agaggacggc cgcgcgaggc cacttccatc ccccaatggc
      121 acaaggcgcg gatttgatca cgtgagcacc aataacgtac aacgctggcc tcttcagggt
      181 cttgatcctc ggttaagagc tcgcgtgcga tgaggaccac caggtcgttt tctgcgtcgt
      241 accccagccc taactcgcca gtccggaaga gcggatccag gggcggatgg atgtgcatgt
      301 tctcctctac gtattccgag gaggccttgg tgagattggg aaaacgctcg taaatctctg
      361 ccaggaattg ttccagaccc accgccaggg attgaatctg gatcttctct acgatcagtg
      421 ttacggtgcg ctcttcctgc cagccctgaa tgtaaaaaat acgcttacca ggcgggccga
      481 tagcgtcggt tgtaatgtgg ctgaccggac gaaggtcgat ctctttagtg ggcattaggc
      541 tctcctgcct gggatactga gcgtagtata ccagaatcaa ggtttggaca ggttaaaaga
      601 aagatcatag ttcagcgcaa gcaggtagct gcccatttgg ccgacttgca gggcggtaat
      661 cgaagctggt gcaataaata gtcgttggaa ctgatccagg ggaaggccaa ggtagtaggc
      721 cacagccaat ttgatcgggt cggcgtgaga cacgcaaacg aagatatcct tctcttcgta
      781 ctgggcgcgt aatgtttcaa tttctccaac catgcgcgcc tgacattcgg cgaatgattc
      841 gccgccaggg aagcggaaga gcgagggcgt actctgcacg atgcgccaga ctttcaggcg
      901 gcgcagactt ttgtggcttt taccctgcca ctcgccgata tcggattcca tcagacctgg
      961 tcgtacaatg gtctcctgac ctaatgcctt ggccaggtat tcggcagtct ccgcagcccg
     1021 ttccagtggg ctggtgtaga ttgccttgat gggcgcttgt cttagctttt ctgctacggc
     1081 ttgtgcctga gcgcgcccgt tatcattgag gtgaatgccc ggcatacgcc ctggcataat
     1141 tcctttctta acgaagtcgt tttccccgtg tcgaattaat aatattgtgg gcatttactt
     1201 agcctgcctt gtgtttgtgc ttgatctctt ggatttcgtc ttgggatggt tcgggaagct
     1261 cagttgtctg ggtgataccc aacgctttgc gctgggcatc tcgccagcgg ctaagcctat
     1321 cgatgacctg agactcgtaa ccctgtgatg acggtttgta gaaataggtc cccaaaagcg
     1381 ggtggggaag atactgctgt ggtaagaagt gctctggatc ttcatgcgga tattggtaac
     1441 cctgcccgtg cccgaggggc ttggcgtcgc ggttggcgtc ttgcaagtgc tgggggacgg
     1501 cagtaatgcc ctcgtcctcg atgagctgga aggccttgaa ataggaggtg gctgtgttgg
     1561 atttcggtgc ggtggccagg taaagcgtgg cctctacgat ggggtagatc ccctcgggta
     1621 agccaatata atcgaaggat tgggcggcag cgttggcgat caccagccca agtgggtcag
     1681 ccagaccgat atcctcccca gccaggatga tcagcctgcg caggatgaag cgcgggtctt
     1741 ccccggcata aagcatcttc gccagccagt agagagcggc atccggatca gagccgcgca
     1801 cagatttaat gaatgccgag atggtgtcat aatgggcatc gccatccttg tcgtagagca
     1861 cagcacggcg ctggatggat tcttgcgcca cctgcaatgt gatgtgtatc gtctcctgat
     1921 cgccagcggg ggtgctctca accgccagtt ccagtgcgtt taaggcattg cgggcatcac
     1981 ctccagctac gcggatcagg tgctctaaag catcttcgtc aagcttgatg tgctttgccc
     2041 catagccgcg ttcgtcgtcc tgcaaagcat attccagtat gcggcgcaca tgctcgtcct
     2101 ccagagggcg gagctggaag acgcgcgagc gcgagaccag cgcgccgatc acctcaaagt
     2161 acggattttc agtggtcgcg ccgatcaggg taatgttgcc attttccacg tggggtagca
     2221 gagcgtcctg ctgggctttg ttccagcgat gcacctcatc cacgaagaga atagtgcgcc
     2281 ggttgtgcag cttgcgcctc tcgatggcag cctcgataac ctttttaagc tcagccttgc
     2341 cagctagcac tgctgaaaga gtctcgaagt gcatgcgggt gtgattagct atgaccatcg
     2401 ccagggtggt cttaccgctg cccggcgggc cccacaggat gatggacgag aacaggcggt
     2461 cggactctat ggcgcggcgc agcagccgcc ccggcccgac gatctcctcc tgcccgacaa
     2521 actcgtccag gatgcgaggg cgcattcgcg ccgccagagg cgcttcggat ttcatacgtt
     2581 cgtgcatggc gtgggagaag aggtccatgg aggagattat aacgccccat caagtcaggg
     2641 gcaagcgaag aaatgctcat ctaacgggct gggcatcact ttggactatt cttgagcacg
     2701 cgggtagagc ttgtcctggc atgatgactt ttctgatgtt gtgctatact ctcaccaaaa
     2761 tactcattaa aatattgtgc agcctattgt attttatgtc ttagaggtct gttagatgtt
     2821 gagcagctga atcgcagcga acgacagtct tgcagccgaa atactttctg tttcgcttca
     2881 tgtgtagggc tgaacccgac tgttaggcag caccttctat cctatgacaa ccgctgcttg
     2941 tcaagcggtg catggcccaa actgacatcg tcaaatcatc taataggaaa cattcacatg
     3001 taatctaagc gtcatagtct tagataaggc gtacccgata cgtgtaagga gctcgttcac
     3061 atgggaagct cacaggaatc taagaccatc agagtgctcc tcgtttgcgc ctttggattc
     3121 tctaccgccc tcttggaggc acggattact gaggcggcga aagctgctgg agttaatcta
     3181 gacattcgcg gagacacggt ggacggcatt ccgttgattg attttgaaaa gaaaatcttt
     3241 gatgtgatct tgctggcccc acaggtgcgc ttcatgagga agaggattgc ccagagggtc
     3301 gcgccattca acatcgtcgt tcaagtgctc gacccgatgg cttttgggat gatggatgga
     3361 gaactcctct ttcgacagat actcgaagca ttgcaggctc acgaagcaac agaattatcg
     3421 tcattatcct gagtttatcg tgctgaagag atgacaactt ttgtatccca tctaccacag
     3481 acaagcactt gtctggccgg cccgcagata gcatttgtgc gataattatc tttggctagc
     3541 tgatacgcca gcttgtagca gttgagcggt cggaattact accgacgaaa gggttcaatt
     3601 ggctcccgat aacacgatta ccctctacaa gtggattttg aaggaattta ccattgctga
     3661 gcgtggcaaa gcagtgcaga aaatagggcg ccaccttgct ccattggtcc aagctggtga
     3721 ccaagtcctg gatctttgct gtgggacagg gccagcatct ttttggtttg aagaacgggg
     3781 cgctgcggtt accgccatag attttgcccc ttatatgat
//
LOCUS       DBNHOH010000277         2362 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1682971, whole genome shotgun sequence.
ACCESSION   DBNHOH010000277 DBNHOH010000000
VERSION     DBNHOH010000277.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1682971"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1607
                     /locus_tag="AC16V7_04940"
     CDS             <1..1607
                     /locus_tag="AC16V7_04940"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HIR6256499.1"
                     /translation="SFDHKVIGIQYGVTALILFTLAGAYALTFRTELLAPEIQFLQPK
                     NFNTTMSLHGIVMIGAMLIGVGAMSNYLVPLMIGANDMAFPRLNAFAYWFNVPAAVLL
                     TLVWFLGGFDTGWTGYPPLSAKAPLGMQMFFLGVYFIGFSSIFGSLNIIVTIVKMRAK
                     GMSYFRMPIFVWTAFATALIGLTATQLIGLSFQMVMFERLFGMGFFEPSKGGDPILFQ
                     HLFWFYSHPAVYVFVLTGLGVISELLPVFVRKPLFGYRWVALSSFGIALVGFLVWAHH
                     MFTSGMAAYLRVPFMYSTLLVAVPTGIKFFSWVATMWEGKMSFETPMLFVLGAISIFL
                     LGGLSGPPNGVVSTDLHLQDTYWIVGHFHATMFGGFVFPFFAAIYYWFPKVTGRMYNE
                     RLGKIHFYLMLPAFYVQSLGQMQVGLLGMRRRIADYDPALDINLSHQLITLAGFFIGI
                     SVLIAFYNFITSLRRGEVAVANPWRSRSPEWQIPSPVPAHNFDQPFEVVGEPYDYGLE
                     GSAYVSFTPSRPSTEVPSGVEPAAAD"
     gene            1691..1918
                     /locus_tag="AC16V7_04945"
     CDS             1691..1918
                     /locus_tag="AC16V7_04945"
                     /inference="COORDINATES: protein motif:HMM:NF015582.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HIR6256500.1"
                     /translation="MDEKELEEKKLDAFRVGVGVLILLGVMTIGEYWLGAVASAWWSP
                     LLGVALLKAFFVVRDYMHLPRLFAPEEGAHE"
     gene            1915..>2362
                     /locus_tag="AC16V7_04950"
     CDS             1915..>2362
                     /locus_tag="AC16V7_04950"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HIR6256501.1"
                     /translation="MTTHTSTLVEYRRKVAANRLGLWLFILSDTFVFGGLYVSRFYLL
                     GTQERPEVSQLLGFVVTAILLLSSYFMNRAETAMAHGDRKTFLRGTLTTLILGVIFLV
                     GVVGVEWQIAPFGPGDGAAASIFYTMTGFHAFHVLTGVIFLAVVYNN"
BASE COUNT          397 a          648 c          681 g          636 t
ORIGIN      
        1 tctcgtttga tcacaaggtg atcggcattc aatacggggt cactgcgctg atcctgttca
       61 ctttagcggg tgcctatgcc ctcacattcc gcaccgagct gcttgccccg gagatccaat
      121 tcctgcagcc gaagaatttt aacaccacca tgagcctgca cggcatcgtg atgatcggag
      181 ctatgctgat cggcgtaggg gcgatgtcga actacctggt gccgttgatg atcggcgcca
      241 acgacatggc tttcccccgc ctgaacgcct tcgcttactg gttcaacgtt ccggcggctg
      301 tcctgcttac cctggtgtgg ttcctgggcg gttttgatac aggctggacg ggctacccgc
      361 cgctgagtgc caaagcgcct cttggcatgc agatgttctt tttgggcgtg tattttatcg
      421 gtttttcttc gatctttggg tcgcttaata tcatcgttac catcgtcaag atgcgcgcca
      481 agggcatgag ctacttccgc atgccgatct tcgtttggac agccttcgcc acagctctca
      541 tcggcctgac cgcgacccag ctcatcggtc tgtctttcca gatggtcatg tttgagcgcc
      601 tgtttggtat gggtttcttc gagccgtcca aaggtggcga cccgatcttg ttccagcacc
      661 tgttctggtt ctactcgcac ccggcggtgt acgtcttcgt gctcaccggg ttgggggtga
      721 tcagcgagct gctgccggtc tttgtgcgca aaccattgtt tggctatcgc tgggtggcgc
      781 tgtcctcctt cggtattgct ctggtgggat tcttggtctg ggcgcaccat atgttcacct
      841 cgggcatggc ggcttacctg cgtgtgccct tcatgtatag caccctgctg gtggctgtgc
      901 cgacgggtat taaattcttc agctgggtgg ctaccatgtg ggaaggcaag atgagcttcg
      961 agacccccat gctgttcgtg ctgggcgcca tctcgatttt cctgttgggt gggctgagcg
     1021 gcccgcctaa cggtgtggtc tccaccgacc tgcacctgca agacacatac tggatcgttg
     1081 ggcacttcca tgccaccatg tttggcgggt ttgtcttccc cttctttgca gccatttatt
     1141 actggtttcc aaaagtcacc gggcgcatgt acaacgagcg gctgggcaag atccatttct
     1201 acctgatgct gccggctttc tatgtgcagt cgttggggca gatgcaggtt ggcctgctgg
     1261 gtatgcgccg ccgcattgca gattacgatc cggccttgga tattaacctg tcgcaccagt
     1321 tgatcaccct ggcaggtttc tttattggta tctcggtgtt gatcgccttc tacaacttca
     1381 tcaccagcct gcgccggggt gaggtggcgg ttgccaaccc gtggcgctcg cgctcacccg
     1441 aatggcagat cccctcgcca gtgccagcgc acaatttcga ccaacctttc gaggtggtcg
     1501 gagagccgta cgactatggt ttggaaggct cagcgtatgt cagttttacc ccatcccgcc
     1561 cctccacgga ggtgccgtct ggggtagagc cagccgcggc tgattgagct ggagtgtaga
     1621 gatccttgac tgaaggctga gcatcagccc ttcatcacga aatgttgtgg agtatttcag
     1681 gagttaaatc atggacgaaa aagaattgga agagaagaaa ctagacgctt ttcgcgtggg
     1741 cgtgggtgtg ctcatcctgt tgggcgtgat gaccatcggc gagtactggc ttggcgcggt
     1801 ggcttctgcc tggtggtcgc cactgctggg cgtggctctg ctgaaagcct tttttgtggt
     1861 gcgcgattat atgcacctgc cgcgactctt tgcccctgag gagggtgcgc acgaatgaca
     1921 acacatactt caacacttgt cgaatatcgc cgcaaagtgg cagctaaccg cctgggattg
     1981 tggctgttca tcctttcgga tacttttgta tttggtggac tgtacgtcag tcgcttctac
     2041 ctgcttggca cgcaagagcg ccctgaagtc agccagctac tgggttttgt agtcaccgcc
     2101 atacttcttc tgagcagcta tttcatgaac cgggctgaga ccgccatggc gcacggggat
     2161 cgaaagacct tcttgcgtgg cacgctgacg acactgattc tgggtgtgat ctttctggtc
     2221 ggcgtggtgg gagtggagtg gcagattgcc ccctttggcc ctggtgatgg agccgctgcc
     2281 tcgatctttt acaccatgac gggtttccac gcttttcacg tgctcaccgg cgtgatcttc
     2341 ctggctgtgg tgtacaacaa tg
//
LOCUS       DBNHOH010000278         7520 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_335780, whole genome shotgun sequence.
ACCESSION   DBNHOH010000278 DBNHOH010000000
VERSION     DBNHOH010000278.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7520)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7520)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7520
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_335780"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="AC16V7_04955"
     CDS             <1..459
                     /locus_tag="AC16V7_04955"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR6256502.1"
                     /translation="GLLRNLAILLAHSGDSWFWLLGLVLLWWLGGDFWQGRALELGVG
                     ILVTAVLVMLIKFSVRRRRPVGEWGKIYRNTDPHSFPSGHAARAILLAVMAVGLGPGW
                     FALLLWFWAPLVVLARVAMGLHYFSDVLAGAGLGFVMGIVILRFLPAFPF"
     gene            complement(425..1492)
                     /locus_tag="AC16V7_04960"
     CDS             complement(425..1492)
                     /locus_tag="AC16V7_04960"
                     /inference="COORDINATES: protein motif:HMM:NF006426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HIR6256503.1"
                     /translation="MNLDDLAYFSKVDIQHMHDQINSLPDQLEKAWSLGSSQSLPNWQ
                     GIRQVVIAGMGGSAIGGDLLSAYVEPFCKVPVVVHRDYGLPAWGRGPETLVIASSHSG
                     NTEETLSSYEQAQANNCRILAISTGGKLAEAAKNAGTPLWVFEHHGQPRAAVGYSFGL
                     LLSALAHLDLVPDHSDEIMDAAAAMKAQQAQLVASTPVVKNPAKRIAGQLMGRWVTVF
                     GAGILAPVARRWKGQVNELAKAWGQYDLLPEADHNTLAGILNPPEQLPRMVALFLRSP
                     SDHPRHRLRLDLTRKSFMLEGISTDFIDATGDSRLANQWTGLHFGDYTAYYLAMAYGI
                     DPTPVDPIETFKREMQAESAK"
     gene            complement(1498..2334)
                     /gene="rsmI"
                     /locus_tag="AC16V7_04965"
     CDS             complement(1498..2334)
                     /gene="rsmI"
                     /locus_tag="AC16V7_04965"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006516138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR6256504.1"
                     /translation="MGILYLVATPIGNLEDISARALRILGEARLIAAEDTRHTRKLLS
                     HFGIHTPMISYHEHNKLTRLDEVLAALAQGDVALVSDAGTPALNDPGYELVGAAIAAG
                     YTVSPIPGPTAPIAALVASGLPTDAFLYLGYLPRKAAERRNLLAQVASMPFTLIFLET
                     PHRLMGALEDMQSKLGDRPCAIARELTKLHEEIYRGSLGEALRHFGAQKPRGEFTIVV
                     AGSSIQQEPRWTEEKLRSALQSGLDQGESPSNLAKRIASKSGWSRREIYRMIANKHER
                     EE"
     gene            complement(2334..3404)
                     /locus_tag="AC16V7_04970"
     CDS             complement(2334..3404)
                     /locus_tag="AC16V7_04970"
                     /inference="COORDINATES: protein motif:HMM:NF027484.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HIR6256505.1"
                     /translation="MKLSLIRSSLLAFLILAIVSGCNIPRREASSTDINVTQAYETIQ
                     ARLTQNSQSNTSSQGTPTPTTGLTSPTPLTTPPTITPTREPTANQPTATSRFSCDQAS
                     PGNPIDVTIPDDTPLQPGQSFTKVWRLQNTGTCTWNNTYAVAFFSGDQMGAPASVALA
                     GNVAPGQSVDISVDMVAPSKAGNYQGNWKLRNASNVLFGIGPGGAAPFWVRIVVSVTS
                     TPSSTPRTPTPTSTPTPTATATSPVHIVGTANLNVGATLDLDNDQINGGGADIIYEPI
                     DSKHQLAPQDSTLLGVYGMSQPGQANCQSSSLDTSPIVVEDIPLNSYICYRTHEGRYG
                     RARLSEFNAVNFTVRLEFLTWP"
     gene            complement(3423..4574)
                     /locus_tag="AC16V7_04975"
     CDS             complement(3423..4574)
                     /locus_tag="AC16V7_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009247 - glycolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGDG synthase family glycosyltransferase"
                     /protein_id="HIR6256506.1"
                     /translation="MKHPVPGKPHILILFSDTGGGHRSAAEAIIEALHLEYEEQFTIE
                     MVDIFKDIALPPLNRMPDLYPTMVRIPQAWGFGFRLTNGSQRSRLLTSSTYPYVRKAL
                     RKLIAKHPSDLIVSVHPLANAPILRSLGKDRPPYITVVTDLVTAHALWYHRLTDLCLV
                     PTEAARQRALRYGLKPDQVKVVGLPVANRFCIAAGDPVELRNQYGWRQDRPVILLVGG
                     GEGMGPLEKTARAIADAGLNASLVVITGRNESLKARLESMSWSIPTHIYGFVREMPNF
                     MRAADILVTKAGPGTICEALNAGLPMILYSRLPGQEEGNVSYIVSEGAGVWAPQPEKI
                     VATLHNWITHPQELAQAEAACRRLARPQAARQVAHILAGFVGIQYQPGA"
     gene            complement(4552..4764)
                     /locus_tag="AC16V7_04980"
     CDS             complement(4552..4764)
                     /locus_tag="AC16V7_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HIR6256507.1"
                     /translation="MLPNIQLDDIVRLRKPHPCGSYEWRIVRLGADIGLVCLGCGRRV
                     LLDRRTLAKRMKKILPKTSDETSGAG"
     gene            complement(4849..>7520)
                     /locus_tag="AC16V7_04985"
     CDS             complement(4849..>7520)
                     /locus_tag="AC16V7_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009547575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR6256508.1"
                     /translation="GPNELEERGVKSPWLILWEQLTETMVVILIISAAISSILGDLKD
                     AVAILAIVVINTLLGFRQEYQAEKAMAALKKLAVPVVKVRRGGHVLEISSRDLVPGDI
                     VLLEAGNLVPADCRILESVNMRTQEAALTGESEPVSKSSQAQPGKSLPLAERYNMVYM
                     GTLVTYGRGIGVVAETGMRTELGNIAELIQSAAQEPTPLQQRLNRLGKQLAWVALGLV
                     ILVFLLGVARGEELRLLFLTAISLAVAAVPEGLPAVVTISLALGARRMLRRQALIRKL
                     PAVEALGSVTVICSDKTGTLTENRMTVTVIDLAEHRIDLSEEETRGYFSENLEPSHSE
                     TPGHQQLAYLNENPALTVMLAGSVLCNDAYLEYDENTSQRFHIVGDPTEGALMVAAAR
                     MGLRREALEAIMPRVAEAPFDSDRKRMTTLHELKSEQLEGWQETLLELLNLQPENLRL
                     IAFTKGAMDGLLEVSDRVWVQNHIEPLDAGWRKRIEDANERLAMEGVRVLGVASRAVD
                     DQPEGDDVFKLEQGLIFIGLVGMIDPARAEVKQAVQTCITAGIRPIMITGDHPLTARY
                     IARELEIDIENGVPTGNDLDRMSAQELEETVGKVSVFARVSPEHKLKIVQALQNRGQI
                     AAMTGDGVNDAPALRQADIGVAMGITGTDVSKEASDMVLLDDNFATIVAAVEEGRVIY
                     DNIRKFIKYTFTSNAGEIWVMILAPFLGMPLALLPLQILWINLVTDGLPGLALGIEKA
                     ERNTMRRPPYPPNENIFARGVGRDILFIGALMGLLSLGVGFWYWRMGHPTWQTMVFTT
                     LTLSEMGYVLAIRSNRDSLFTIGVFSNWALIGAVILTTLLQIAVVYVPFLQSIFETMA
                     LPVRDLGIAMLLSTSLFIVVELQKQLMRRLSPK"
BASE COUNT         1605 a         1985 c         2176 g         1754 t
ORIGIN      
        1 ggcttgttgc gcaacttggc aattcttctg gctcactcag gagattcctg gttttggctc
       61 ctgggtctgg ttttattatg gtggttgggt ggagatttct ggcaagggcg agctctggag
      121 ctgggggttg gtatcctggt gacggctgtg cttgttatgt tgattaagtt ttctgtgcgc
      181 agacggcgac ctgtggggga gtggggcaaa atttaccgca acacagaccc gcactcgttc
      241 ccatcgggtc atgcagcccg agctattctg ctggcggtta tggctgttgg attggggcca
      301 ggttggtttg cgctgctgct ctggttttgg gcaccgctgg tcgtattggc tcgggtagcg
      361 atgggtttgc actacttctc agatgtgctg gctggtgcgg ggttggggtt tgtgatgggg
      421 atagttattt tgcgctttct gcctgcattt cccttttaaa cgtttcgatt ggatccacag
      481 gcgttgggtc gatcccataa gccatagcca agtaataagc ggtgtagtca ccgaaatgca
      541 gcccggtcca ttggtttgcc agccggctat caccagtcgc gtcgatgaaa tcggtgctaa
      601 tgccctctaa catgaaactt ttgcgggtca aatcaaggcg caggcgatgt cgggggtgat
      661 ccgatgggct acgcaggaat agcgccacca tgcgcggtaa ttgctctggg gggtttagga
      721 tgcccgccag ggtgttatgg tcggcttccg gcagaagatc gtattgtccc caggccttgg
      781 cgagctcatt aacctgccct ttccagcggc gggcgaccgg ggcaagaatc cccgcgccga
      841 ataccgtcac ccagcgcccc atgagctgcc cagcgatacg cttggcagga ttcttgacta
      901 cgggtgtgct ggcgaccagc tgggcttgct gggctttcat cgcggcagcg gcatccatga
      961 tttcgtcgga gtgatctggg accaaatcaa ggtgagctaa tgcggaaagc agcaacccga
     1021 aggaatagcc gaccgcggcg cgtggttgtc catgatgttc aaaaacccat aatggtgtgc
     1081 cagcattttt agcagcttcg gccagttttc caccggtgga tatagccagg atgcggcaat
     1141 tgttggcttg ggcttgctca tatgatgaga gcgtctcttc ggtattgccg gaatgagatg
     1201 aggcgatcac cagcgtctcc gggccgcgcc cccaggcagg caagccataa tcgcggtgaa
     1261 cgacgacagg cactttgcag aaaggttcca cataagcgga tagcaaatca cccccaattg
     1321 ccgagccacc catacccgca atcacaacct ggcggatgcc ttgccagttg ggaagtgact
     1381 ggctcgagcc caacgaccaa gctttctcaa gctggtcagg caagctgtta atctggtcgt
     1441 gcatgtgttg gatatcgact ttggaaaagt aagccaggtc gtctaagttc atttgtttta
     1501 ctcctctctc tcgtgtttat tggcaatcat ccgatagatt tcccgccggc tccagcctga
     1561 ttttgatgcg atgcgcttgg ctagattaga tggggattcg ccttgatcga ggccagactg
     1621 caaggcggag cgcagtttct cttctgtcca gcggggttct tgttgaatgg aactccccgc
     1681 cacgacgatc gtgaattcgc cgcgcggctt ttgagcgcca aagtgtctca atgcttcgcc
     1741 caggctgcct cgatagatct cctcgtgcaa tttggttagc tctctggcta tggcgcatgg
     1801 acgatcgccc agttttgatt gcatgtcttc aagggcaccc atcaagcggt gaggtgtttc
     1861 caggaatatc aatgtaaatg gcatgctggc tacttgtgca agcagattcc gccgttccgc
     1921 ggccttgcgc ggcaggtagc caagatataa aaaggcatct gtaggcaacc cagaagccac
     1981 cagggcggcg atgggcgcag ttggcccagg gatcgggctg acggtgtacc cggcggcgat
     2041 agccgcgcct accaactcat atcctggatc gtttaaggcc ggtgttcccg catcggaaac
     2101 cagcgcaacg tccccctggg ccagcgccgc cagcacctcg tccagtcggg tgagcttatt
     2161 atgctcatga tagctgatca tcggggtgtg gatgccaaag tgagagagca gcttgcgtgt
     2221 gtggcgtgtg tcttcagctg caatcaaacg cgcctcgccc aagatgcgca gggcacgagc
     2281 gctgatatct tcgaggttgc cgatgggagt agcaaccaga tacagtatac ccattatggc
     2341 caggtcagga attccagtct aacggtgaaa ttcactgcgt tgaattcaga caggcgagcg
     2401 cggccgtaac gtccctcatg agtgcggtag caaatatagg aatttaatgg gatatcctca
     2461 accacaatag gagacgtgtc gagggagctg gactggcagt tcgcttgccc tggctggctc
     2521 atgccatata ctcccagcag ggtgctatct tgcggggcta actggtgctt gctgtctatg
     2581 ggttcgtaaa tgatgtctgc gccgccgccg ttgatttgat cattatccag gtcaagagta
     2641 gctcccacat tcaagttggc tgtgccaacg atatgtactg gggaggtggc tgtggcggtg
     2701 ggtgtcggcg tggaggtggg ggtaggggtg cgcggcgtac tgcttggcgt ggatgttacg
     2761 gataccacga tacgcaccca gaacggcgcg gcaccacccg gtccgatacc gaagaggaca
     2821 ttggaggcgt tgcgcagttt ccagttgccc tggtaattac cagctttgga tggagcgacc
     2881 atatccacgg aaatatccac gctctgtcca ggtgctacgt tgcctgcgag agctacactg
     2941 gctggtgcgc ccatttggtc gccggaaaag aatgccaccg cgtaggtatt attccaggta
     3001 caggtacctg tgttttgcaa acgccagact ttggtgaagg attgacctgg ctggagaggc
     3061 gtatcatcgg ggatagtcac atcgattggg ttgccaggag atgcctggtc gcaagagaag
     3121 cgggatgtcg ctgtgggctg gttggcggtc ggctcgcggg tgggagtaat ggtgggaggc
     3181 gtagtgagag gggttgggct ggtgagacca gttgttgggg tgggggttcc ttgcgaagac
     3241 gtgttggatt gtgaattctg ggtcagcctg gcctgtatcg tttcatacgc ttgtgtgaca
     3301 tttatgtcgg ttgacgaagc ctcacgcctg ggtatattac aacccgatac aatggcgaga
     3361 ataaggaagg ctagcaggct cgaacgaatt aacgacaatt tcatgggttg tcctgatgga
     3421 gtctacgccc caggttggta ttgtatcccc acaaaccctg ccaggatgtg ggcgacctgg
     3481 cgggcggcct gcgggcgtgc tagccggcga caggcagctt cggcctgggc gagctcttgt
     3541 ggatgagtga tccaattgtg cagggtagcc acaattttct ctggctgggg tgcccaaacc
     3601 cctgccccct cggatacgat atagctcaca ttgccttcct cctgacccgg aaggcggctg
     3661 tagagtatca taggcagccc tgcgttcagc gcctcacaaa tcgtgcctgg gccagctttg
     3721 gtgaccaaaa tatcggccgc acgcatgaaa tttggcattt cgcgcacaaa gccgtaaata
     3781 tgggtaggta tggaccagct catggactcc aggcgggctt ttagactctc gtttcgtccg
     3841 gtgatcacaa ccagcgaggc gttcaagcca gcatcggcga ttgcgcgagc ggttttctcc
     3901 agaggaccca tgccctcacc accgcctact aacaggatga cgggtcgatc ctgccgccag
     3961 ccatattgat ttcgtagctc aaccggatct ccagccgcaa tgcagaagcg gttagcaacg
     4021 ggtagcccca ccacctttac ctggtcaggt ttcaacccat aacgcagggc gcgttggcgg
     4081 gcggcctctg tgggcaccag gcataaatcg gtgagacggt ggtaccaaag agcgtgagcg
     4141 gttaccagat cggttacgac agtgatgtag ggaggcctgt ctttgcctaa agagcgcagt
     4201 ataggtgcat tcgctagagg atgaacggat acgatcaggt cacttgggtg ctttgcaatc
     4261 agcttgcgca gcgctttgcg tacataggga taggtgctgg aggtgagaag gcgcgatcgt
     4321 tgagatccat tggtgagccg gaagccaaag ccccaggctt ggggaatcct gaccatcgtc
     4381 ggatacaaat ccggcatgcg atttagtgga ggcagagcaa tgtctttgaa aatatccacc
     4441 atctcgatcg tgaattgctc ttcgtattcc aaatgcagcg cctcgatgat cgcctctgcc
     4501 gcgctacgat gcccaccgcc agtatcggag aataatatca gtatatgagg tttacccggc
     4561 accggatgtt tcatcacttg ttttcggcaa tatctttttc atgcgcttgg caagcgtgcg
     4621 tcggtcaagt agcacacgcc gaccgcagcc taagcatacc agcccaatat ctgcgcccag
     4681 tcggacaatg cgccactcgt aactgccgca tggatgcggc ttgcgaagtc gaacaatatc
     4741 gtccagttgg atatttggga gcatcaagcg cattataaac tgaaaatcgc tctttgcgga
     4801 tgcttgcatc gagctggatg tgctcgggcg caggggtaga acgcctggtc attttggtga
     4861 tagacgtcgc atgagctgct tttgcaattc aaccacaata aacaaggatg tgctaagcag
     4921 catggcgatg cccaagtcgc gcacgggcaa ggccatcgtc tcgaaaatgg attgcaagaa
     4981 gggtacatag accactgcga tttgtaataa ggtagtcagt atcactgcac cgataagcgc
     5041 ccagttgcta aaaactccga tggtgaagag agaatctcgg ttagatcgga tagccagaac
     5101 atagcccatt tcagataagg tcagggtagt gaagaccatc gtctgccagg taggatgtcc
     5161 catgcgccag taccagaagc ctacacccag ggagaggagc cccatcaacg cgccgatgaa
     5221 caaaatatcc cgacccaccc cgcgcgcaaa gatattctca ttgggcgggt aaggggggcg
     5281 tcgcatagta tttctctcag ccttctctat gcctaacgct aatcctggca gcccatcggt
     5341 gacgaggttg atccaaagga tttgcagcgg gagcagggct agaggcatac ccaggaaagg
     5401 tgccaggatc atgacccaaa tttctccggc attgctggta aatgtatatt tgatgaactt
     5461 gcggatgtta tcgtagatca cccgcccctc ttcgacagct gccacgatgg tggcgaagtt
     5521 gtcgtccagc agcaccatat cggaagcttc tttggataca tccgtgccag tgatgcccat
     5581 ggctacaccg atatcggctt gtcgaagagc tggcgcgtca tttaccccat ctccggtcat
     5641 ggccgcgatc tgcccccgat tttgcagcgc ctgcacgatc ttcagcttgt gttccggcga
     5701 aacgcgggca aacaccgaga ccttcccaac ggtttcctcc agctcttgcg cagacatacg
     5761 atccaaatca tttcctgtgg gcacaccgtt ttcaatatca atctccagct ctcgggcgat
     5821 atagcgagct gtgagcgggt gatccccggt gatcatgatc ggccggatac cagccgttat
     5881 acatgtctgg acagcctgct ttacctcagc gcgggcggga tcaatcatgc ccaccaggcc
     5941 aatgaaaatt aggccttgtt ccagtttgaa aacatcgtcg ccttctggtt gatcgtcaac
     6001 agcccggctt gccactccga ggacgcgcac tccttccata gccagacgtt cgttggcatc
     6061 ttcgatacgc ttgcgccagc ctgcatcaag tggttcgatg tgattttgca cccacacccg
     6121 gtcggatact tccaggagtc catccatagc acccttggta aatgcgatca ggcgcaggtt
     6181 ctcgggttgc aggttgagca gctccagcag cgtctcctgc catccttcga gctgctcact
     6241 tttgagttca tgcagggtgg tcatgcgttt tcgatcggaa tcgaaaggtg cttccgcgac
     6301 gcgcggcatg atcgcttcaa gggcttcgcg cctcaagccc atgcgcgccg cggcaaccat
     6361 caaggctccc tcggttgggt cgccaacaat gtggaagcgt tgcgaggtat tttcatcata
     6421 ttccagatac gcatcgttgc acaacacact gcctgcaagc atcacggtca aggcaggatt
     6481 ctcattcaaa tatgcgagct gttggtgacc tggtgtttca gaatgggacg gctccagatt
     6541 ctctgaaaaa taacctcgag tttcttcctc actgagatca atgcggtgct cggctaggtc
     6601 aatcacggtc actgtcatgc ggttctcggt cagtgtgccg gttttatcag agcagatgac
     6661 cgtcacggag cccaacgcct caacggcggg caatttgcgg ataagggctt ggcggcgtag
     6721 catgcggcga gcacccagtg ccagggaaat tgtgactaca gccggtaggc cctcggggac
     6781 cgctgcaaca gccaaactta tggcagtgag gaatagcaag cgcaactctt cgccacgcgc
     6841 cacccccagc aaaaatacta atatgaccaa gcccaacgct acccaggcca gctgctttcc
     6901 gagccggttc aggcgctgct ggaggggggt gggttcctgg gcagcagact ggatcagttc
     6961 agcaatatta cctaactcgg tgcgcatgcc tgtctcagcg accacaccaa tgccgcgccc
     7021 gtaggttacc agggtgccca tatagaccat gttataccgt tctgccagcg gcagcgattt
     7081 acccggctgt gcttgcgagc ttttggaaac tggctcggat tcaccggtta gggctgcctc
     7141 ttgagtgcgc atgttaacgc tctccagtat gcggcagtca gctggcacca ggttacctgc
     7201 ctcgagcaaa acgatatcgc ctgggaccaa atcgcgagaa gagatttcga gaacgtgccc
     7261 gcctctacgt actttgacaa ccggcactgc tagtttcttc agcgcggcca ttgctttctc
     7321 tgcttggtat tcctggcgaa agcctaacaa ggtgttaatc acaacgattg ccaggatggc
     7381 aactgcatcc ttaagatccc ccagaataga tgagatagca gccgagataa tgaggatcac
     7441 caccatggtt tccgtgagtt gttcccacaa aatcagccag ggtgacttaa cccctcgctc
     7501 ctccagctca ttggggccgt
//
LOCUS       DBNHOH010000279         2373 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1746712, whole genome shotgun sequence.
ACCESSION   DBNHOH010000279 DBNHOH010000000
VERSION     DBNHOH010000279.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1746712"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="AC16V7_04990"
     CDS             <1..337
                     /locus_tag="AC16V7_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256509.1"
                     /translation="AQGFSQEALRLLAWMGAQPDLTTHMAARRRIIEAGAYRLSARYL
                     LDGDKPGPALLAYGRALIRNPRFALQHWHRMIYAVLSLVGGKSLANLYYRYRKPSPRP
                     SAPPLKGNG"
     gene            350..1288
                     /locus_tag="AC16V7_04995"
     CDS             350..1288
                     /locus_tag="AC16V7_04995"
                     /inference="COORDINATES: protein motif:HMM:NF024860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HIR6256510.1"
                     /translation="MHLTKDRRNKPILVTGGHRTGTTWVGRMLTASSEAAYISEPLNV
                     LHRPGVMRASVRYWYTYICPDNEAAYLPALRQTLNFRYHLAAEIVSLRSRRDALRMAR
                     DWWAFFRGRFLRLRPLLKDPFAVFSAPWFAQRLGCTVVITVRHPAAFASSLKRLEWSF
                     QLEDLLAQPLLMRDWLEPFRADMQAMPAGDIIGRASLLWKMIYQVVDTFRSLHPQFQI
                     VRHEDLSLEPASGFRNLYNSLELDFTPQVSTAIQNSSSAENPKELASSAVHAVRLDSR
                     ASLDNWKHRLSIEEVTRIHRITAGVVENFYDESSWG"
     gene            1292..2077
                     /locus_tag="AC16V7_05000"
     CDS             1292..2077
                     /locus_tag="AC16V7_05000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR6256511.1"
                     /translation="MAEQPLVSIVTPSFNQAHYLEETIHSVLAQSYPHLEYIIVDGGS
                     TDGSVEIIKKYANRLAWWINEPDRGQTDAINKGFARAQGEILAWLNSDDTYLPHAVAE
                     AVETLQAHPEAGMVYGDANLVDEAGQVIGRFPARQTDYRRLRRGYVHIPQQAAFFRAS
                     LWREVGPLDPTFFFAMDYDLWVRLAKVSALLYTPRLWANFRLHSHGKTVVSDDRCWPE
                     MLRVHYREGGSPLSLLTLKAKLRPLLYAWLPLRLRLWIRRWAR"
     gene            2139..>2373
                     /locus_tag="AC16V7_05005"
     CDS             2139..>2373
                     /locus_tag="AC16V7_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013746712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6256512.1"
                     /translation="MRNILVTGGAGFIGSNIVKALNDRGREDILVVDEPGNEAKLANL
                     ADCTYLDLVNKDDFLASLDDGTLRGGGVETVFHE"
BASE COUNT          485 a          745 c          681 g          462 t
ORIGIN      
        1 ggcacaggga ttcagccagg aagccctgcg cctgctggcg tggatgggtg cgcaacccga
       61 cctgaccacc cacatggctg ccaggcgccg tatcatcgag gcgggtgcct atcgcctgag
      121 cgctcgctac ttgctggacg gggacaaacc cggcccggcg ctgctggctt acgggcgcgc
      181 cctgatacgt aacccgcgct tcgccctgca acactggcac cgtatgatct acgccgttct
      241 cagcctggtt gggggcaaga gcctagctaa tctctattat cgttacagga aaccatcccc
      301 gcgtcctagt gctcccccgc ttaaagggaa tgggtaagag acaaaaagaa tgcatctcac
      361 caaagatcgc cgaaataagc ctattctcgt caccggcggg caccgcaccg gcacgacatg
      421 ggtgggtcgc atgctcaccg ccagcagtga ggcagcctac atcagtgagc cgctcaacgt
      481 cttgcaccga ccaggggtga tgcgtgcctc ggtgcgatac tggtacacct atatttgccc
      541 ggataacgaa gctgcttacc tgcccgcact gcgccagact ctgaacttcc gctaccacct
      601 ggcagcagag attgtctccc tgcgctcgcg ccgtgacgcc ctgcgtatgg cgcgtgactg
      661 gtgggcattc tttaggggtc gtttcctgcg attacgcccc ctgttgaagg atccatttgc
      721 cgtcttctcg gcaccctggt tcgcccaaag gttgggctgc acggtggtga tcaccgtgcg
      781 ccacccggca gctttcgcca gcagcctcaa gcggctagag tggagcttcc agctcgaaga
      841 cctgctcgcc cagccgctgc tgatgcgcga ctggcttgag cccttccgcg ccgacatgca
      901 agccatgcct gcgggagaca tcatcgggcg cgccagcctg ctatggaaaa tgatctacca
      961 ggtggtggat acgtttcgct ccctgcaccc ccagttccag atcgtgcgcc acgaggacct
     1021 atcgctcgag ccagccagcg gttttcgcaa cctttacaat tccctggagc ttgatttcac
     1081 cccccaggtc agcacggcga ttcaaaactc cagcagcgcc gagaacccca aagagctggc
     1141 gtcatccgca gttcatgccg tccgcctgga tagccgcgcc agcctggaca actggaagca
     1201 ccgcctcagc atcgaagaag tcacccgcat ccatcgcatc acagccggcg tggtcgaaaa
     1261 cttctacgat gaaagctcgt ggggttagcg catggctgag cagcccttag tatccattgt
     1321 caccccttca ttcaaccagg ctcactacct ggaggagacc atacactcgg tgctggcgca
     1381 gagctacccg cacctagaat atatcatcgt cgatgggggc tccaccgatg gcagcgtgga
     1441 gatcatcaag aagtatgcca accgcctggc ttggtggata aacgaacccg accgcgggca
     1501 gaccgacgcc atcaacaagg gcttcgccag ggcacaagga gagatcctgg cctggctgaa
     1561 ctctgatgat acttacctgc ctcacgccgt tgcggaggcg gtggaaactt tgcaggctca
     1621 ccccgaggcg ggcatggttt atggcgatgc aaacctagtg gacgaggctg gacaggtgat
     1681 cgggcgcttc ccggcaaggc aaaccgatta ccgccgcctg cggcgcggct acgtgcatat
     1741 cccccagcaa gccgccttct tccgggcaag cctgtggcga gaagtaggcc cgctcgaccc
     1801 taccttcttc ttcgccatgg attacgatct gtgggtccgg ctggcaaaag tatccgcgtt
     1861 gttgtatact ccccgcctgt gggcgaactt ccgcctgcac agccacggca aaaccgtggt
     1921 ctcggatgac cgctgctggc ctgagatgct gcgcgtgcat tatcgagaag gcggcagccc
     1981 gctctcgtta ttgaccttaa aagccaaact ccgcccgtta ttgtatgcct ggttgccgct
     2041 tcgcctgcga ttgtggatcc gccgatgggc taggtagtgt atccacgctc gaaaaattgg
     2101 agaaaagaga tacaattcaa ggcgaaaaaa ggaatttcat gcgcaatatc ttagtcaccg
     2161 gcggggccgg gtttatcggc agcaatatcg tgaaggcgct caatgaccgg gggcgcgagg
     2221 acatcctggt ggtcgacgag ccgggcaacg aggccaagct cgccaacctg gccgattgca
     2281 catatctcga cctcgtcaac aaggacgatt tcctcgcctc gctggatgac ggaacgctgc
     2341 gcggcggggg tgtcgagacc gtgtttcacg agg
//
LOCUS       DBNHOH010000280         1870 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_721362, whole genome shotgun sequence.
ACCESSION   DBNHOH010000280 DBNHOH010000000
VERSION     DBNHOH010000280.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_721362"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1485)
                     /locus_tag="AC16V7_05010"
     CDS             complement(<1..1485)
                     /locus_tag="AC16V7_05010"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR6256513.1"
                     /translation="MKDARPKTLSGGRHRLGLPSLVATLVLVFSMLACSTGSYWVPPK
                     GTDTPPPDISFLLEPTPTEITILDPPPGDIATPVTDPPLPQDTPVPPAINTTPVLYYT
                     QAADTLPVVAVRFGVRPEEITSPDPVPEEALLPPNQLLIIPRRLVNAASSQRIFPDSE
                     VVYSPSAIDFDIQAYVQEAGGYLSTYKEWLQTTKTTTGADIVARVAVENSINPRLLLA
                     LLEYQSGWVYGQPSDLKMADYPMGLVNLNKKGLYQQLVWAINHLSVGYYSWREGRLLE
                     LRFADGVTARLAPDVNAGSAALQYYFSQIYTGEQWVRAIDLQDGLPALYERMFGSPWM
                     RAQAVEPLYPPGLAQPELILPFEVDRLWSYTGGPHGAWEHDGSYAALDFAPASSEPGC
                     QQSNAWVTASASGLVVRSGNGVVVLDLDGDGYEQTGWVVLYLHVSTQSRAPVGTWVGA
                     GDHLGHPSCEGGMATGTHVHIARKYNGEWIAADGPMSFQLSGWMA"
     gene            complement(1448..>1870)
                     /locus_tag="AC16V7_05015"
     CDS             complement(1448..>1870)
                     /locus_tag="AC16V7_05015"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR6256514.1"
                     /translation="DLLGCIVSNDWVTAVADGLILRAENGAVIQDLDGDGYEQTGWVV
                     LYMHVESRERVSPGTFLKAGERIGHPSCEGGISSGTHVHLARRYNGEWIPADQNLPFV
                     LDGWVSRGAGLEYDGYLQKDGYEVEAYEGRSAENAIRR"
BASE COUNT          425 a          550 c          552 g          343 t
ORIGIN      
        1 ggccatccaa ccagagagct gaaatgacat ggggccatcc gcggcgatcc attcgccgtt
       61 gtatttgcgg gcaatgtgga cgtgcgtgcc ggttgccatc cccccttcgc aggagggatg
      121 acccaaatgg tcgccagcac ccacccaagt ccccaccggg gcgcggctct gcgtagaaac
      181 gtgcaaatag agcaccaccc atccagtctg ctcgtagcca tccccatcta gatcgaggac
      241 aaccaccccg ttgccagaac gcaccaccag cccagaggcc gaggcggtca cccaggcatt
      301 agactgctgg caacctggct cggagctggc aggggcaaag tccaaagcag cgtaagagcc
      361 gtcgtgctcc caggcaccgt ggggaccgcc ggtatagctc cacaagcggt caacttcgaa
      421 gggcaggatg agctcgggct gggccagacc gggagggtaa agcggctcca ctgcctgggc
      481 gcgcatccaa ggggagccga acatgcgctc gtacaaggca ggcaaaccgt cctgcaggtc
      541 gatggcgcgc acccactgct caccggtgta gatttgtgaa aagtaatatt gcagggcagc
      601 gctgccggcg ttcacatccg gtgccaggcg ggcagtgacc ccatcagcga agcgcaactc
      661 caacaaccga ccctcccgcc agctatagta accgaccgag agatggttga ttgcccagac
      721 aagctgttgg tagagccctt tcttgttaag gtttaccaaa cccatggggt aatctgccat
      781 tttgagatcg gagggctgac cgtagaccca gccgctctgg tattccaaca aagcgagcag
      841 caagcgcggg ttgatggaat tctcgactgc cacccgagcc acgatatcgg cacctgtggt
      901 ggttttggtg gtttgaagcc actctttata ggtgctcaga taaccaccag cctcttgtac
      961 atatgcctgg atatcaaagt cgatggcgga cggcgaataa accacctcgc tgtcagggaa
     1021 tatacgctgc gaagaggccg cgtttaccag tcgacgtgga atgatcagca gctggttagg
     1081 tggcagcagg gcttcctctg ggacagggtc aggggaggtg atctcttcag ggcgcacacc
     1141 aaagcgcacc gctacaacgg gcaaggtatc tgcagcctgg gtgtagtaaa gcacaggtgt
     1201 ggtgttgata gcaggcggaa caggcgtatc ctggggaaga ggcgggtcgg tcaccggagt
     1261 ggcgatatcc cctggtggag ggtccagaat cgtaatttcc gtgggggtag gctctaacag
     1321 gaaggagata tcgggtgggg gcgtgtcggt gcctttggga ggcacccagt aactgcctgt
     1381 ggaacaggca agcatagaaa aaaccagcac cagagtcgcg accagggaag gcaagcccag
     1441 acgatgccta ccgccggata gcgttttcgg ccgagcgtcc ttcataggcc tccacttcgt
     1501 aaccatcttt ttgcaaatat ccatcgtatt ccaatcccgc gccgcgcgac acccagccat
     1561 ccaggacgaa gggcaggttt tggtcagcgg ggatccactc tccattataa cgccgggcaa
     1621 ggtgcacatg cgtgccagac gagatgccac cctcgcagga gggatgaccg atacgctcgc
     1681 ctgctttgag gaacgttccc ggacttacac gttcccggct ttcgacgtgc atgtacagca
     1741 ccacccagcc agtttgttca tagccatccc cgtccaagtc ctggatgaca gccccatttt
     1801 cagcacgcaa gatcaatcca tcggcgacag ccgtcaccca gtcattgctt acgatgcacc
     1861 ccagcaggtc
//
LOCUS       DBNHOH010000281         3938 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1683211, whole genome shotgun sequence.
ACCESSION   DBNHOH010000281 DBNHOH010000000
VERSION     DBNHOH010000281.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1683211"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..1423)
                     /locus_tag="AC16V7_05020"
     CDS             complement(122..1423)
                     /locus_tag="AC16V7_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6256515.1"
                     /translation="MRLKNIAILGAGIGGMAAAYDLRRAGHQVTIYEAADFVGGLASG
                     FKEPHWDWYVEKYYHHWFASDRHILGLIDELGWRDQVLFPRPYTVVYHEGQFYPFDSI
                     LTALMFPGLGWGIHKLRFGLVGLYLKLTNRWQPLESVTVDDWMRRWAGDRVYELMWEP
                     LIIGKFGERYAKQVNMAWLWARLKARTTRLGTFKGGFQTFANRFANSLREMGVHIHLS
                     TPINHIEPQAAGLLTLTTPTNPVQFDQCLVTTSPALLARLAPSLPPEYLSGLLNLKSM
                     GAVVMVLSLKHQLSEQGYYWYNLPKAAGFPFLALVEHTNYLPPEHYGGDHLVYAGDYL
                     ETDHEYFSLSQDELLQRFVEVLPRFNPNFTPDWVRKSWLFRTNYAQPVPFVNHSKNIP
                     AIQTPIPGLYFASMSQVYPWDRGTNFAVEIGRRAAGIMLGE"
     gene            complement(1420..2448)
                     /locus_tag="AC16V7_05025"
     CDS             complement(1420..2448)
                     /locus_tag="AC16V7_05025"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR6256516.1"
                     /translation="MRRWQFWLGVVISLLFLYVALRGLHLEDLEASFTEANYWWLIPG
                     VGVYFLGVWARAWRWHYLLRPVKPIPTRVMFPIVAIGYMGNNIYPARAGEVLRAVVLK
                     RRQGVPVSASLATIIVERVYDGVVMLAFVFLNLPKLARLTADSGFVGNIQTLALWGAG
                     AFVGALGVFLLAAMFPEQAEAILTTLVQRLVPARIRDRTLSLGQRFLGGLQALRSPRE
                     AMMVFFTSVVIWLLETGKYWFVMHAFPFEVSFFALMLMNGIVNLATTIPSAPGYVGTF
                     DAPGIAVLAAYGVERAIAAAYTLVLHVALWVPITALGAYYFAREGLHWGEGVGEIEKD
                     EVEGVKVS"
     gene            2549..3058
                     /locus_tag="AC16V7_05030"
     CDS             2549..3058
                     /locus_tag="AC16V7_05030"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIR6256517.1"
                     /translation="MLTLNQLAPDFLLPDLDGTLHSLRNYRGRVVVLNFWSAECPHAA
                     RTDAQLVANQKTWGSEVVLISIASNANEPPELLRQVANQEGLTLILLDQGSDVADRYG
                     AITTPHLFVIDEKGILRYQGAFDDINFRKRSATKHYLYDAVEAVLAGALPNPAETPPY
                     GCAILRHVI"
     gene            complement(3111..>3938)
                     /locus_tag="AC16V7_05035"
     CDS             complement(3111..>3938)
                     /locus_tag="AC16V7_05035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR6256518.1"
                     /translation="AIQTGGLRVDSILGDFIVKPAQASDEPSQVGVVDVVILGVKAWD
                     VPNAAQAMRPMVGDQTFVVPLENGVNAPEQLASVLGREHVMGGLCQISAFIAGPGHIR
                     HVGIEPFVAFGELDGRPSQRAEGLLQVLEAAGVKAAIPPDIQVSMWEKFLFIATVSGL
                     GAVTRAPADIYRSLPETRQMLIAAFEEIVALARARGVMLPEDTVEKRLAFVDNLPDGL
                     LASMQRDILEGKPSELASQNGAVVRMGAELGVPTPVHSFIYNCLLPQELRARGQLKF"
BASE COUNT          902 a         1131 c         1082 g          823 t
ORIGIN      
        1 ctcttcaacg ggcgctaatt cggtggcagc ttgcagtact ttctgccctg atggggtaag
       61 caaattgcga aaagtagcta aatccacacc tgcattttat caggcaactg gctcccattg
      121 cctattcgcc gagcatgatt ccggcagccc tgcgcccaat ctctacagcg aagttggtcc
      181 cccgatccca cgggtatacc tggctcatgc tggcaaagta aagcccgggg atgggtgtct
      241 gaattgcggg gatgtttttc gagtggttca caaagggcac aggctgggca tagttggtgc
      301 ggaaaagcca ggatttacgc acccaatcgg gcgtgaagtt ggggttgaag cgcggtagca
      361 cttccacgaa gcgctgcagt aactcgtcct ggctcaggct gaagtactcg tgatcggttt
      421 ccaggtaatc gccagcatac accaggtggt ctccaccgta atgctccggc ggcaggtaat
      481 tggtgtgctc caccagggca aggaagggga agcccgccgc tttgggcagg ttgtaccagt
      541 aataaccctg ctcagatagc tggtgcttaa gggagagcac catgaccact gcgcccatgc
      601 tttttagatt gagcaggccg cttaaatact ctggcggtag agatggcgcc aggcgcgcca
      661 gtaatgccgg cgaggtggta accagacatt gatcgaattg gacagggttg gtgggggttg
      721 tcagggtgag caagccggcg gcctgtggct cgatgtggtt gataggtgta gacagatgga
      781 tgtgcacacc catctcacgc aaagagttgg cgaaacggtt ggcaaaagtc tggaagcctc
      841 ctttgaaggt gcccagtcgg gtggtgcgag ctttcaagcg cgcccacagc caggccatgt
      901 tgacctgctt ggcgtatcgc tcgccaaact tcccgatgat caatggctcc cacatcagct
      961 cgtaaacgcg gtcaccagcc cagcggcgca tccagtcgtc tacggtgaca ctttccagag
     1021 gttgccagcg gttggtaagc ttgaggtaga gcccaaccaa gccgaagcgc agtttatgga
     1081 taccccagcc aagccccggg aacatcaagg cggtcaggat cgaatcgaag ggataaaatt
     1141 gcccctcgtg gtacaccaca gtgtaagggc gcggaaaaag cacctggtcc cgccagccca
     1201 gttcatcaat caagcccagg atatgacggt cgctggcgaa ccagtggtgg tagtacttct
     1261 caacgtacca atcccaatgc ggctctttga aaccagatgc cagcccgccg acaaaatctg
     1321 ctgcctcgta tatggtcacc tggtgaccgg cgcgcctcag gtcataggcg gcagccatgc
     1381 cgccgatgcc agcgcccagg atggcgatat ttttcagtct caactgactt ttaccccttc
     1441 cacttcgtct ttttcaattt cacccacccc ctctccccag tgcaaaccct ctctggcgaa
     1501 gtaatatgcc cccaaggcag tgatcgggac ccacagggcg acgtgcaaga ccagcgtata
     1561 ggctgcggct atggctcgct ccactccata ggctgctaga acggcgatac caggcgcatc
     1621 gaacgtgccc acgtaacccg gcgctgaagg gatggtggta gctaagttga cgatgccgtt
     1681 catcagcatc agagcgaaaa agcttacctc gaatgggaaa gcgtgcatca cgaaccagta
     1741 cttgcctgtt tctagcagcc agatgacaac agacgtaaag aaaaccatca tcgcttcgcg
     1801 cggtgagcgc agtgcctgta atcctcccag gaagcgctgc cccaggctga gggttcgatc
     1861 gcggatgcgt gctgggacca agcgttgcac aagggtcgtc agaattgctt cggcttgttc
     1921 cgggaacatg gcagccagaa gaaagacccc cagcgcaccc acaaatgcgc ctgcacccca
     1981 cagtgccagg gtctggatat tccccacaaa gcccgaatca gcggtcagcc gggccagctt
     2041 gggcaggttg aggaacacaa aggccagcat gaccacacca tcgtatacgc gttccacaat
     2101 gatagttgcc agagaggctg atacgggaac gccttggcgg cgtttaagca ccacagcgcg
     2161 cagcacttcg cctgctcggg caggatagat gttattgccc atatatccga tggcgacaat
     2221 agggaacatc acacgtgtgg gtattggttt gaccggtcgg agcaagtaat gccagcgcca
     2281 ggcgcgtgcc cacaccccaa ggaagtacac gcccacccca ggtatcagcc accaataatt
     2341 cgcttctgtg aaggaggcct ccaaatcttc cagatgcagg ccgcgcagcg cgacgtataa
     2401 aaaaagcagg ctgatgacca cgcccaacca gaattgccag cgtctcatgg cgctcattct
     2461 accaccgatg ccttatcact tcgtctttca ttctagttgg gctggttgca gctcaatacc
     2521 cgcgcagaga ttgatttata atctcaccat gcttacactc aatcaattag cccctgactt
     2581 cttattacct gatcttgacg gtaccttgca ttcgctgcgg aattaccggg gacgggttgt
     2641 ggtgctcaat ttctggtcgg cagaatgccc tcatgcagcc cgtaccgatg cccagttggt
     2701 cgcgaatcag aaaacatggg gatctgaggt agtgctcatc tcgatcgcca gcaatgccaa
     2761 cgagccccct gaactcctca ggcaggttgc caaccaggaa ggccttactt tgatcttact
     2821 ggatcaggga agtgatgtgg cggatcgtta tggggcaatc acaacgcccc atctatttgt
     2881 gatcgatgaa aaagggattt tgcgctacca gggcgctttt gacgacatca actttcgcaa
     2941 acggtcagca acgaagcact atctgtacga cgcggtggaa gccgtcctgg ctggcgcgtt
     3001 gcccaacccg gcggagacac ccccctatgg ctgtgctatc ttgcgacatg ttatttgatc
     3061 attgggggta taggcagtta ataaggaaca acccccaatt gagtaatttt ttagaatttc
     3121 agttgaccgc gggcgcgcag ttcctgaggc agcaagcagt tatagatgaa ggagtgcact
     3181 ggcgtgggga cgccaagctc tgcccccatg cgcaccacgg cgccattttg tgaagccaac
     3241 tcagaaggtt ttccctccag gatatcccgt tgcatcgatg ccagcaagcc atccggtaag
     3301 ttgtcgacga aagccaggcg tttctcgact gtatcttcgg ggagcataac cccacgtgcc
     3361 cttgccaggg caacgatttc ttcaaatgcc gcaatcagca tctggcgtgt ttcaggcagg
     3421 ctgcgataaa tgtctgccgg agcccgtgtt accgcgccca atccactcac cgtggcgatg
     3481 aacaagaatt tttcccacat cgatacctgg atatcgggcg ggatagcggc ttttacaccg
     3541 gcggcctcca aaacttgtaa caggccctca gcgcgctggc tggggcgccc gtctagctca
     3601 ccgaaggcca caaaaggctc gatgccgaca tggcggatat gccccggccc ggcgataaaa
     3661 gccgagatct ggcacagacc ccccatgaca tgctctctcc ctaaaaccga ggcgagctgc
     3721 tcgggtgcat ttacgccatt ctccagcggc acgacgaagg tttggtctcc caccattggg
     3781 cgcattgctt gagctgcatt gggcacgtcc caggccttca cccccaagat tacaacgtcc
     3841 accacgccga cctggcttgg ctcgtcgctt gcctgggcag gttttacgat gaaatcgccg
     3901 agtatgctat ccacgcgcaa gccaccggtt tggatggc
//
LOCUS       DBNHOH010000282         1391 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_657042, whole genome shotgun sequence.
ACCESSION   DBNHOH010000282 DBNHOH010000000
VERSION     DBNHOH010000282.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_657042"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="AC16V7_05040"
     CDS             complement(<1..997)
                     /locus_tag="AC16V7_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-sulfuration enzyme family protein"
                     /protein_id="HIR6256519.1"
                     /translation="MHIETQCIHAGQHPDPATGAVMTPIYQTSTYVQDKIGLHRGYEY
                     SRTGNPTRTALEACLTDLEGGQYGLAFSSGMAATDAVLRLLSPGDHVVASDDVYGGTY
                     RLFEQVLRPFGLDFTYVDTSNLAQIRAALRPQTRLVWLETPTNPLLSVSDVHAIAEIC
                     QAHPSLPLLVVDNTFATPFLQRPLELGADIVVHSTTKYLGGHSDVVGGALVVKDAALH
                     QRLAFLQNAVGAVPGPMDCFLVLRGLKTLPVRMERHAANAARIAEYLHAHPQVSRVLY
                     PFHDSHPQVETARRQMRSGGGMISFVPNGGAQAAHRMAESTRIFALAESLGGVESL"
BASE COUNT          332 a          362 c          392 g          305 t
ORIGIN      
        1 tcagcgattc gactcctcca agagattctg ccagggcaaa gatgcgcgtg gattcggcca
       61 tacgatgggc agcctgagcg ccaccgttgg gcacgaaaga gatcatgcca cctccggaac
      121 gcatctgtcg ccttgcggtc tctacctgtg gatgcgaatc gtgaaagggg tagagcacac
      181 gcgacacctg ggggtgggca tgcaggtatt cggcgatgcg ggcggcgttg gcggcgtggc
      241 gttccatgcg caccggcagg gttttcaatc cgcgcagcac caggaagcag tccatcggcc
      301 ctggcacggc accaacagcg ttttgtaaaa atgccagtct ctgatggagc gcagcatctt
      361 taaccaccaa cgcccctcca accacatccg agtgaccgcc gaggtatttg gtggtggagt
      421 gcactacaat atcagcgcct agctccagtg gcctttgtaa gaaaggcgtg gcgaaggtat
      481 tatcaacgac caagagcgga agggatggat gcgcctggca tatctctgcg atggcgtgta
      541 catcgcttac gctgagcagc gggttggtgg gtgtctccag ccaaaccagg cgggtctgtg
      601 gacgtaaagc ggctctaatt tgagccaggt tggatgtgtc cacgtaggtg aaatccagcc
      661 caaagggacg taaaacttgc tcaaacaacc ggtaggtacc gccataaaca tcatcgctcg
      721 ccaccacatg gtcacccgga gagagcaggc gcagcacagc gtccgtagct gccatacccg
      781 aggaaaatgc cagcccgtat tggccacctt ccaaatctgt aaggcaagct tccagcgctg
      841 tgcgcgtggg gttgcccgtg cgtgaatact cgtaaccgcg gtgtaatcct atcttatcct
      901 gaacataggt tgacgtctga taaatgggcg tcataacggc tcccgtagca gggtcagggt
      961 gctgcccggc gtgaatgcat tgcgtttcga tatgcatgga ttgatcctcc ttcgcgaaca
     1021 tcattgtagc caaggttggg ctagagggga agtgaattaa tgagatattt tcaaaccctc
     1081 gtagctctct aaggtaagcc ttctctgaag acgggtgaca ataaaactgt ctgccctttt
     1141 acatgattag gggagaagtt tgattctttg gtttgtgttc gctagcagac cgcctctggc
     1201 aagggttata atcatctgga aaaattacca aaggaccaat catggatatt ttctttcaag
     1261 atcccacggc aattcccttg ccgcccaacg aagtacgtat aaaagagttg cttgcggagc
     1321 cctggccaga taatcgtcgg gtgcgaataa ccctggaagt gactcccttt caaaaacgcc
     1381 ccaatgggca a
//
LOCUS       DBNHOH010000283         1447 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1042104, whole genome shotgun sequence.
ACCESSION   DBNHOH010000283 DBNHOH010000000
VERSION     DBNHOH010000283.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1042104"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..1256)
                     /locus_tag="AC16V7_05045"
     CDS             complement(96..1256)
                     /locus_tag="AC16V7_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR6256520.1"
                     /translation="MDKCINFQGFMRYLLDDEKLAEKGARIAKALLEAQSPRLTDISE
                     KMSGKGSSRYKEIQRFLKRADLKQSLKRLYQEDAEFVIGDPTEMERYKAPKTPYVGTL
                     KDGTTAGYWLLVLSTPFRGRSLPFSFVVYSSRTIGEQCTSRNQEHYRCFNEVKELLGD
                     RPLVLDREFSYEDLMEILHIENIQFVIRLNLGDQRKQPRLIDADGEPIKLFIRPGETI
                     IRPNVYYLGTVKVNLIGYWHKSLSKPLWVMTSLDPKQGLEIYQKRMKIEQTFRDCKDL
                     LHLTKLMNKKQSLLEQMIALSLLAYVIGVWFGEALRDVVYAKLDIAQVPDALLNKPQV
                     DANKHPKWLLYSGLFVLLKQKPRLSKKQVDAVTIAAASAFALLVCGNVRSFV"
BASE COUNT          342 a          362 c          352 g          391 t
ORIGIN      
        1 cttggattgg tcatctttgt acctcctttc tgagtgtttg gtcacaccca tggaagcaca
       61 agatgaccgt ttttgtcaat cctttttact gaggttcaga caaaagatcg gacattgccg
      121 caaaccaaga gggcgaaagc agaagcagca gcaattgtga cggcatccac ttgtttcttg
      181 gataaacggg gcttttgctt gagcagaaca aacaagccag aatagagtaa ccacttggga
      241 tgcttattgg catcaacctg tggcttgttg agcagagcat cgggcacttg agcaatatcc
      301 agcttggcat aaaccacatc gcgaagagct tcaccgaacc agaccccgat cacgtaggcc
      361 agcaatgaga gcgcaatcat ctgctccagg agcgactgtt tcttgttcat tagcttggtg
      421 agatggagca ggtctttgca gtcgcggaag gtttgctcga ttttcatgcg cttttggtag
      481 atctcaagcc cctgcttggg gtccagactg gtcatgaccc ataggggttt ggaaaggctc
      541 ttgtgccagt agccgatcag gttaactttc acggtcccca aatagtacac attggggcgg
      601 ataatcgttt caccaggtcg aatgaacaac ttgattggtt cgccatccgc atcgatcaag
      661 cggggctgct tgcgctggtc gcccaggttc aagcggatga cgaactggat gttttcaatg
      721 tgcaagattt ccatcaggtc ttcgtagcta aattcccgat ctaacaccaa ggggcgatca
      781 cccagcagct ctttgacttc attgaaacag cgatagtgct cctggttgcg cgaggtgcat
      841 tgttctccga tcgttctcga tgaatacacc acaaacgaga agggtaggga tcgcccacga
      901 aagggtgtcg agagcaccag caaccagtat cctgccgttg tcccatcttt caaagtgcct
      961 acatagggcg ttttgggagc cttgtaacgc tccatctcgg ttgggtctcc gatcacgaac
     1021 tctgcatctt cctggtacag gcgcttcaag gattgtttca aatcggctct cttcagaaaa
     1081 cgctggattt ctttatatct gctgctgccc ttcccagaca tcttctccga tatatccgtc
     1141 aggcgtgggg attgggcttc taataaagct ttggcaatcc tggccccctt ttctgctagc
     1201 ttctcgtcat cgagtagata ccgcataaat ccctgaaaat tgatacactt gtccatggaa
     1261 gcctcctttg agtatctcgt tgagagcggt tactcaattt agaggcttct atctgttttg
     1321 gcaagaccct gtccgaactc ttgtccgaag gtcaggaaaa ctacttgaca gattccacaa
     1381 tacccttaaa atcgggttga atggaatact cttatgtccg tcatttatca gggtttcgcc
     1441 ccgcagc
//
LOCUS       DBNHOH010000284         2536 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_849339, whole genome shotgun sequence.
ACCESSION   DBNHOH010000284 DBNHOH010000000
VERSION     DBNHOH010000284.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_849339"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="AC16V7_05050"
     CDS             <1..856
                     /locus_tag="AC16V7_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6256521.1"
                     /translation="TTMRILTGYMPPTSGRATVAGFDVVEQSLDVRRRVGYLPETVPL
                     YPEMTVFDYLKFMADLRKLNNAEDRVYDVLDLVHMTDRAEGYIANLSKGMRQRIGVAQ
                     ALLHEPEVLILDEPTIGLDPAQILEVRNMIRELGRERTVLLSTHILSEAQQVCNRVLI
                     INKGRIVAEDTPENLQSRLAGAKRVSLRVRGDVEGLEELLAKIPGVLRIFTSGDGGLE
                     FETAPGQDVRPEVARTAVNQGYDLLELRSIGLSLEDIFLQLTREEPEPPSMAADDDFV
                     QAEEGEEE"
     gene            861..1595
                     /locus_tag="AC16V7_05055"
     CDS             861..1595
                     /locus_tag="AC16V7_05055"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6256522.1"
                     /translation="MRNVWTIANREYKLYFASPVAYMVAFFLLLILGIIFYVNVAGAI
                     VQQFPPNIQGVLGPLMTLLLFTTPAITMRLLAEEQRLGTMELLLTAPVRDWELVVGKW
                     LGGFLFLLTILAVTWLYPIFLNQMVDPGIDQGPLITGYLGVILFTASLVAIGVAVSSL
                     FSNQIAVFFATVGVYLLLWLISLPSQASGSATANELLRYLDFSDHFYNTFYQGIIDVS
                     DVIYYLSLTALALFLGSVSVESRRWR"
     gene            1595..>2536
                     /locus_tag="AC16V7_05060"
     CDS             1595..>2536
                     /locus_tag="AC16V7_05060"
                     /inference="COORDINATES: protein motif:HMM:NF021351.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HIR6256523.1"
                     /translation="MKPEWRRFAPVGLYLAILAALTSISLYVIQREWNLYLQISLGVV
                     LLGLAIFALLDPGRVRTALTGRQARYGSNALVMAVAFVGILVVVNYLGYKNSKRWDLT
                     EDKQNTLATETLDTLANLPEPVEALGFYTARISRESAENLLEKYKFEGKGKFNYRFID
                     PEADPIAAKDAKITRDGMIVMKMGDRQESLEFVSEKEMTSALVRLISGETRSVYFLTG
                     HGERDPEGVGDEAYSLVKLVLEGKNYVTGKLNLLSTANIPDDATVIVIAGPQQPLANE
                     EVEKLKAFVESGGGLVVMEEPLVLTDFADRPDPLATYL"
BASE COUNT          574 a          578 c          708 g          676 t
ORIGIN      
        1 taccacgatg cgcatcttga ccggttacat gcctccaaca tccggaagag caaccgtcgc
       61 tggcttcgat gttgttgaac aatcactcga tgtgcgccga agggtgggct atcttccgga
      121 gactgtcccc ttgtatccgg aaatgactgt attcgattat cttaaattta tggccgacct
      181 gcgcaaattg aacaatgccg aggatcgagt atatgacgta ttggatctgg tgcatatgac
      241 cgatcgggca gaaggctaca tcgccaatct atccaaaggg atgcggcagc gtattggggt
      301 tgcgcaggct ttgttgcacg agcccgaggt attaattctg gacgagccta ccattggcct
      361 agaccctgca caaattcttg aggtgcgcaa tatgatccgc gaactaggtc gggagcgcac
      421 tgtactgctg tccacccata tcctttcgga agcccagcag gtctgcaacc gggttttgat
      481 tatcaataag ggacgcatcg ttgcagaaga tacccccgaa aatttacaat cccgcctggc
      541 aggcgccaag cgcgttagct tgcgcgtccg gggcgatgtg gaaggattag aagaattgct
      601 ggcaaaaatc ccaggtgtat tgcgtatttt tacatctgga gatggcggcc ttgagttcga
      661 gacagcccct gggcaggatg tgcgtccgga ggtagccaga accgcagtta atcaagggta
      721 tgacttgtta gaacttcggt cgataggctt gagcttggaa gatatcttct tgcagctaac
      781 tcgcgaagag ccggagccgc ctagtatggc tgctgacgat gattttgttc aagcagaaga
      841 aggggaagag gagtaattct atgagaaatg tttggacgat tgcaaaccgg gaatataagc
      901 tctacttcgc tagcccggtt gcgtatatgg tggctttctt tttgttgctt attttgggga
      961 taatattcta cgtcaatgtt gccggggcga ttgttcagca atttccccca aatatccaag
     1021 gcgtgttggg ccccctgatg acccttttgt tgttcactac ccccgccatc accatgcgct
     1081 tgctggctga agagcagcgt ttgggtacca tggagttatt attgactgcc ccagtgcgcg
     1141 attgggagtt ggtggtgggc aagtggctag gagggttcct cttcctgctg accatattag
     1201 cagtaacctg gctttaccca atcttcctta atcaaatggt ggacccgggc attgatcagg
     1261 ggccattgat tactggttac ctgggtgtaa tactattcac ggcttccctg gtagcgattg
     1321 gtgtggctgt atcatcgctg ttctcaaacc agatcgctgt ttttttcgct actgtgggag
     1381 tttatctcct cttgtggctg attagtcttc cctcgcaggc ctcaggcagc gctactgcga
     1441 atgaactgct gcggtatctg gatttcagtg atcatttcta taacactttt tatcagggga
     1501 tcattgatgt cagcgatgtc atctattact tgtctctgac tgctctggcg ctctttcttg
     1561 ggtcggtttc ggtcgagtct aggaggtggc gataatgaaa cccgaatggc gtcgctttgc
     1621 tccagttggg ttgtatctgg cgatacttgc agccctgaca tcgatttcgt tatatgtaat
     1681 ccagcgtgag tggaatctat atctgcagat cagcctggga gttgtgctgc tcggattagc
     1741 tatctttgcc ctgttggatc cgggcagggt gcggacagcg cttacaggtc gccaagctcg
     1801 atatggtagc aatgctctgg tgatggcagt cgcctttgtt ggcattctgg tggtggttaa
     1861 ctaccttgga tataaaaatt caaagcgctg ggaccttacg gaagataagc aaaatactct
     1921 ggcaactgaa acgctcgata ccctcgctaa tttgccggaa ccagtcgaag cactgggctt
     1981 ttacaccgcg cggatctcta gggagagcgc tgaaaacttg ctggagaagt acaagttcga
     2041 aggcaaaggc aagtttaatt accgctttat cgacccagaa gctgatccaa tcgctgcaaa
     2101 agatgcaaag attacccgtg acggtatgat cgtcatgaag atgggagacc gccaagaatc
     2161 gctggaattt gtctcagaaa aggagatgac atctgccctg gtacgcttga tttcgggcga
     2221 aacgcggtca gtgtatttct tgaccgggca tggcgagagg gatcctgagg gtgtggggga
     2281 tgaggcatat tctttggtca agctggtgct agagggtaaa aattacgtca cagggaagct
     2341 caaccttttg agcaccgcta atatccctga tgacgccact gtgatcgtga tcgctggacc
     2401 gcagcaaccc cttgcaaatg aagaagtgga gaagctcaag gcttttgtgg agtcgggagg
     2461 ggggttggtg gtgatggaag aacccttggt gctgaccgac tttgctgatc gccctgaccc
     2521 attagcgaca tacctg
//
LOCUS       DBNHOH010000285         2991 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_657099, whole genome shotgun sequence.
ACCESSION   DBNHOH010000285 DBNHOH010000000
VERSION     DBNHOH010000285.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_657099"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="AC16V7_05065"
     CDS             <1..730
                     /locus_tag="AC16V7_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR6256524.1"
                     /translation="AVLAEARRQGALTAAITNAVASDLARQADHVIDLGAGVERSVAA
                     TKTYTSALLAIALLSVCLSDGSEMLDALQRVPQAAFQTLDVEGQVAGIVQRYRYMHDC
                     VVIGRGYNYATAFELALKLKELTYTMVEPYSSADFLHGPLAVIEHGFPAIIIAPSGAM
                     LPELQEFMRTIQQRQAEVLAISDHNETLALSDIPLRLPIAVPEWLSPITAIIPGQLLA
                     MHLASIRNYDPDHPRGLRKVTETV"
     gene            850..2160
                     /gene="melA"
                     /locus_tag="AC16V7_05070"
     CDS             850..2160
                     /gene="melA"
                     /locus_tag="AC16V7_05070"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010496524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HIR6256525.1"
                     /translation="MIKVSIIGAGSNEFSAELTRDILATPALSQGTFALIDIDAERLE
                     LAHRMVERLIEQTGRNWTVEASIERKDIMAGSDYIINAIEVAGLANVRHDYDIPMKYG
                     VDQCIGDTSGPGGLFKALRTLPAWIEILKDAERLAPDCLVMNYTNPMSLTVLAGLRAT
                     RLPIVGLCHSVQHTSMQLSSYLDIPYPQIRWQVAGINHLAWFVELSRDGEDLYPRLRQ
                     RARLPEVYEQDPVRLEIMLHLGAFPTESSGHVSEYVGYFRKRPDLLQKYMRSGYRGES
                     GFYANNWPTWRQRGDQRTHAYLRGDGSYPLERGVEYASYIIEAIETNLPTVIYGNVLN
                     SGLITNLPQDGVVEVACLVDRRGIQPTHHGPLPAQLAALNYPHMAFHNLVVTAVLERD
                     REAAIHALMVDPLTSAVCSLDEIRRMFEEMVEAQKAFLPEFLAA"
     gene            2560..>2991
                     /locus_tag="AC16V7_05075"
     CDS             2560..>2991
                     /locus_tag="AC16V7_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256526.1"
                     /translation="MNKKLLLIPLVVLVLLLAAVPIALAGDEACDTRVNNTFAKLLEC
                     VTLDGVREHQAAFQAIADANNGIRTSGTPGYDQSVLYVAGLMEAAGYNVTVQPFEYQT
                     FITLSPSVLEQVSPVAGPVVNNIMSYSGSGDHTAPVSTLPVI"
BASE COUNT          641 a          870 c          828 g          652 t
ORIGIN      
        1 cgctgtattg gctgaggcgc gccgacaggg cgctctgacg gctgccatca ctaatgcggt
       61 tgcctccgac ctggccaggc aagctgatca cgtcattgac ctgggggcag gagtggagcg
      121 gtcggtcgcc gcgaccaaaa cttacaccag cgccctgctc gccattgccc tgctgagcgt
      181 gtgcctttct gatggctccg agatgctgga tgccctgcag cgcgtcccgc aggcggcatt
      241 tcagaccctg gatgtcgagg gacaggttgc cggtatcgtc cagcgttacc gctacatgca
      301 cgactgtgtc gtaatcggac gcggctataa ctacgccacg gctttcgagc tcgctttaaa
      361 gttaaaggag ctgacctaca ccatggtgga accctatagc agcgccgatt ttctacatgg
      421 tcctctggca gtgatagagc acggctttcc cgctatcatc atcgcaccta gcggggcgat
      481 gttgcccgag ctgcaagagt tcatgcgcac gatacagcag cgccaggcgg aggtcctggc
      541 gatcagcgat cacaacgaga ccctggcatt atccgacatc cccctgcggc tgcctattgc
      601 ggtgccggaa tggctctctc cgatcactgc tatcatcccc gggcagctcc tggctatgca
      661 cctggcatcc attcggaact acgatccaga tcatccacgt ggcctgcgca aggtaacgga
      721 gacagtgtga ccttagcccc cacccccgac accttcccca tacgggggag gggagtataa
      781 gcctaatttc catcacattt tcccattccc agctttggtg gatcttatat gtctaccaca
      841 ggagatttta tgatcaaagt gtcgatcatt ggcgcgggca gcaacgagtt ttccgccgag
      901 ctgacccgcg atatcctcgc tacccctgct cttagccagg ggacgtttgc tttaatcgat
      961 attgacgctg aacgcctgga gctggcccac cgcatggtgg agcgcttgat cgagcagacc
     1021 gggcgcaatt ggacggtaga agccagcatt gagcgcaaag atatcatggc tggcagcgat
     1081 tacatcatca atgctatcga ggtagccggg ctggccaacg tgcgccatga ctacgatatc
     1141 cccatgaaat acggcgtgga tcagtgcatc ggtgatacct ccggccccgg cgggctgttc
     1201 aaggcgttgc gcacattgcc agcctggatc gagatcctca aagacgccga gcgtctggct
     1261 cccgattgcc tggtgatgaa ctataccaac cccatgtcgc tcaccgtgct ggccgggctg
     1321 cgcgccacgc gcttgcctat tgtcgggttg tgccactccg tgcagcacac gtcgatgcag
     1381 ttgagcagct acctggatat cccctacccg cagatacgct ggcaggtggc gggcatcaac
     1441 cacctggcat ggtttgtgga gctctctcgc gatggcgaag acctgtaccc ccgcctgcgc
     1501 cagcgcgccc gcctgcccga ggtctatgag caagacccag tgcgcctgga gatcatgctg
     1561 cacctgggag ccttccccac cgaatcgagc gggcacgtct ccgagtatgt cggctatttc
     1621 cgcaagcgcc ctgatttgct gcaaaagtac atgcgcagcg gctaccgcgg tgaaagcggc
     1681 ttctacgcca ataactggcc gacctggcgc cagcggggtg accagcgcac gcacgcctat
     1741 ctacggggcg acgggtctta cccgctggag cgcggcgtgg agtacgcctc ttacatcatc
     1801 gaggccattg agaccaacct ccccacggtc atttacggga atgtgttgaa cagcggcttg
     1861 atcaccaacc tgccgcagga tggcgtggtg gaggtggctt gcctggtgga taggcgcggc
     1921 atccagccca cccaccatgg gccgttgcct gcgcagctgg cggcgctcaa ctatccccat
     1981 atggccttcc acaacctggt ggtgactgcc gtgttggaaa gggatcgaga ggcggcgata
     2041 cacgctttga tggtagaccc gctgacctcg gcggtgtgct cgctggatga gatccgccgc
     2101 atgtttgagg agatggtgga agctcaaaaa gctttcctgc cggaattttt agccgcttaa
     2161 ttgctgggct aacgcagttg gaaagggcat tttgggcgag tgatgcccac tcaaaatccc
     2221 ctccattctt ctttgactac gttaaccata aatagattag tatttgctga caaattctga
     2281 taaatacttg ccaaaatcga taaatttggg tataaacaaa tggggtaatt tatatcgatc
     2341 taggtgaatt ttttcgacaa gactgttctt taatcgagat tcgtaagggc actaaaatat
     2401 cccaataaag ggaggtgatg gattgacagg aataccatgt tcgatttcat actcggaaaa
     2461 ttaatcccat agcaggatct gataaaccaa gcagattcgg caacccaaga agaggctgta
     2521 tagcctctat accaaaccaa cctttggagg aaaattgtaa tgaacaagaa actgttatta
     2581 atcccgctcg tcgtcctggt cctgctgctg gcggctgttc cgatagcgct ggctggcgat
     2641 gaggcatgtg acacacgcgt caacaacaca tttgccaagc tgctcgagtg tgtcacgcta
     2701 gatggtgtgc gcgagcacca ggcggcgttc caggctatcg ccgacgctaa caatggcatc
     2761 cgcacctcgg gcacgccggg ctacgaccaa tccgtgcttt atgtagcggg actcatggaa
     2821 gccgccggat acaatgtcac cgtccagcct ttcgagtacc agacattcat cactctctca
     2881 ccgtccgtcc ttgagcaggt atctccggtg gccggaccgg tcgtgaacaa tatcatgtct
     2941 tactcaggga gtggcgacca cacagcgcct gtgagcaccc tgcctgtgat c
//
LOCUS       DBNHOH010000286         1557 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1105883, whole genome shotgun sequence.
ACCESSION   DBNHOH010000286 DBNHOH010000000
VERSION     DBNHOH010000286.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1105883"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="AC16V7_05080"
     CDS             <1..1165
                     /locus_tag="AC16V7_05080"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA mutase large subunit family protein"
                     /protein_id="HIR6256527.1"
                     /translation="IIWAMYIAVAEKQGVRPDQLRGTTQNDILKEYIAQKEFIFPPEP
                     SMRLVVDTIQFGTTTVPQWNTISISGYHIREAGSTAAQELAFTLADGLEYVRWGLERG
                     LEIDQFAPRLSFFFNAHNDFFEEISKYRAARRIWARELQQTFGAQNPRSWLMRFHTQT
                     AGVSLTAQQPENNIVRVAIQALAAILGGTQSLHTNSMDEALALPSEHAVTIALRTQQI
                     ISEESGVANTIDPLGGSFFVEAQTNQMEKQTYDYFRRIEDLGGVLPAIEKGFFQSEIA
                     DAAYRYQREIDEGLRSIVGVNAFQDEKSVTIPILQMDPQGYRRQVARLEEVRRTRDSG
                     QVGQALDRLRIACQGTENTMPYILDAVRAYATLGEIVSVMKEVFGTYQEPSWI"
     gene            1239..>1557
                     /locus_tag="AC16V7_05085"
     CDS             1239..>1557
                     /locus_tag="AC16V7_05085"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019671440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR6256528.1"
                     /translation="MANVQKKKGIIGILTGGGDVPGLNPAIRAVTFRALREGYDVIGI
                     RRGWGGLIDMIRDPKADNSNNFQILSEEIVNRAGRTGGTFLHTSRTRPSRISKPNVPE
                     HLRD"
BASE COUNT          386 a          426 c          403 g          342 t
ORIGIN      
        1 gatcatctgg gcaatgtaca tcgccgtcgc agaaaagcaa ggtgtgcgcc ctgaccaact
       61 acgcggcacc acccaaaatg atattctcaa agaatatatc gcccaaaaag aatttatctt
      121 ccctcccgaa ccctccatgc gcctggtggt ggatactatc caattcggca caacgaccgt
      181 accgcaatgg aataccatca gcatatccgg ctatcacatt cgagaagccg gatctacggc
      241 agctcaggaa ttggccttca cattagccga cggcctggag tatgtgcgct ggggattgga
      301 gcgcgggctg gaaattgacc agtttgcacc gcgcctgtct ttcttcttta atgcacacaa
      361 cgatttcttt gaagaaatct caaaataccg cgccgcccgg cgcatttggg cgcgcgagtt
      421 gcaacagacc ttcggagctc agaacccacg ctcctggttg atgcgcttcc acacccagac
      481 cgcgggggta tccttaaccg cccagcaacc cgagaacaac atcgtgcgcg ttgccatcca
      541 ggcgctggcg gctatcctgg gaggcacaca atcgctgcat acgaacagca tggatgaagc
      601 cctggcactt ccttccgagc acgctgtaac cattgccctt cgaacgcagc aaatcatctc
      661 cgaagaatcg ggcgtggcga ataccattga tccattgggc ggctcctttt tcgtcgaagc
      721 gcaaaccaat caaatggaaa agcaaaccta tgattacttc cgtcgcattg aagatttggg
      781 tggggtgttg cctgctatcg aaaaggggtt tttccaaagc gagattgctg acgctgccta
      841 tcgctaccag cgggagattg atgaaggctt acgcagtatt gtgggcgtga acgccttcca
      901 ggatgaaaaa tcagtgacta tacccatctt gcagatggat cctcagggtt atcgcaggca
      961 ggttgctcgc ctggaagaag tgcgccggac gcgcgactcg gggcaagtgg ggcaggcttt
     1021 agatcgcttg cgcatcgcct gtcagggtac tgaaaacacc atgccctaca tcctagacgc
     1081 tgtgcgtgct tacgccacgc tcggtgaaat cgtcagcgtg atgaaagagg ttttcggcac
     1141 gtaccaagaa ccatcctgga tctgaaattt caggctgttc aaccgcccgc ttttcccgaa
     1201 cgacctgcat ctttttataa gcatttggag gttgtgtaat ggcaaatgta cagaagaaaa
     1261 aaggaattat tggtatcctg actggtggtg gagatgtccc cggattgaac ccagccatca
     1321 gggctgtgac tttccgagcg ttgcgcgaag gctatgacgt cattggcatc cgtcgcgggt
     1381 ggggtggtct catcgatatg atccgtgacc caaaagcaga caacagcaac aatttccaga
     1441 ttctctccga agagattgtc aaccgcgcag gtaggacagg tggaacattt ctgcacacct
     1501 cgcgcactcg cccaagccgt atctcaaaac ccaacgttcc agagcactta agggaca
//
LOCUS       DBNHOH010000287         4641 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1747139, whole genome shotgun sequence.
ACCESSION   DBNHOH010000287 DBNHOH010000000
VERSION     DBNHOH010000287.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4641)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1747139"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="AC16V7_05090"
     CDS             <1..791
                     /locus_tag="AC16V7_05090"
                     /inference="COORDINATES: protein motif:HMM:NF037660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR6256529.1"
                     /translation="IEEAERASQEANRMIKTISDPEPEAMTITLDCQVRLQLAAENYL
                     EASKLVREHGLDVRDEILYFHELEHIVLARVLIYSGRENPDNGHLLTDAQELIARMLE
                     VAKPAGCMREVTVLLVLQALAYQAQMNYDLALSSLAEALVLAEQEGYVRTFIDEGDQM
                     QELLRLASSQGIATDYIGKLLTAFGSGRLVTQQIASPLIEPLSERELEVLHLLATELS
                     GPEIARELVISLNTMRTHTKNIYSKLNVNSRVAAINKARELNLL"
     gene            988..1230
                     /locus_tag="AC16V7_05095"
     CDS             988..1230
                     /locus_tag="AC16V7_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256530.1"
                     /translation="MPEFYKILIKGHLQDRWEEWLEGLMICRNADGTTTLSGVLSDQA
                     ALHGVLLKIGNMNLKLISVNPIEEYLEDKNTNRKEK"
     gene            1233..1595
                     /locus_tag="AC16V7_05100"
     CDS             1233..1595
                     /locus_tag="AC16V7_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256531.1"
                     /translation="MITEQFFSKIERRSMAESLYWAGVLVWAGLVFGANSLGVLPQFG
                     SGDAWSWVFIGAGLYGTFMNIYHSTLPDPVTTTWDYIWSGFWLLLGLSGLFAIDMFWP
                     LVLVLIGGVTLIKAISNG"
     gene            1703..1942
                     /locus_tag="AC16V7_05105"
     CDS             1703..1942
                     /locus_tag="AC16V7_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256532.1"
                     /translation="MTFKQKTTQEKQTPFDISACMAMMEKMMGEHMAGCDCAEMMSQI
                     TGQAEVPDEWLKVMSQMMELHCQPLEGADKRAQVL"
     gene            2036..3355
                     /locus_tag="AC16V7_05110"
     CDS             2036..3355
                     /locus_tag="AC16V7_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bactofilin family protein"
                     /protein_id="HIR6256533.1"
                     /translation="MNFKTSIQNKWGRLAIFVPVMVGFLTLAFAGSAQAAEVVTGDPD
                     AIVAAGVVINDDLIISGDKLVIDGTITGNLFATGIEVVLNGSVEGSVFIGGELLTING
                     DIGGSLYSGGCAITLGPEATIGRSVYAGGFSLIAESGSHVGYDVHAAVFQNRLNGEVG
                     KDVEVATGAFEFNGSAGGNVDVELGETCPYCLEFVPVEAIQPGLRIADDSTVGGEITY
                     KVTARNYESVDASNIEIQPMEVDFEELRNSVLRFIVVQEIRRAIGEFISLLIVGGLLV
                     YFLKDRVRSVMDKISGSALPSFGWGLLVILLFPLVLMVTLALVIAMAFITGLITLGQL
                     SSIVIGFGGFTIASFVVFFVFVISLVTKAVFAYMLGRLVVGRISKDTLESRWGDVWAL
                     AIGVLIYEIVRIIPIIGLFVAMAVILVGAGAIVRSLRDTLRLSAAIA"
     gene            3450..4154
                     /locus_tag="AC16V7_05115"
     CDS             3450..4154
                     /locus_tag="AC16V7_05115"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6256534.1"
                     /translation="MSVLKNDVAAFIDLDNTLYTKFLWQALFAHHRRNRFKRLVVARF
                     VAFHFPLWQLAKAGLVPKDLFYRINATNLAWLVGGVSIERAERIWDWIIDHEILPNLR
                     PEMQAVIEDHRNQVHRVILISGSFTPLLDKLAIRLGLECAIATPLVVKDGHYTGKIVP
                     PLNIGQGKVERLQRFLNDRGNEIDLEKSYFYTDAFVDAPVLEMFGNPIAVYPDSHLAA
                     LAAERGWSVIGCAHVN"
     gene            4356..>4641
                     /locus_tag="AC16V7_05120"
     CDS             4356..>4641
                     /locus_tag="AC16V7_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256535.1"
                     /translation="MTIEQNTSKKKERQRLEGLWWAVALIWAGLVLGADSMGFLPQIG
                     SADTWSWIFLGAGVFGSLGNLYRMGSSDAPNPTSWDWIWSGAFLILGLGGF"
BASE COUNT         1212 a          994 c         1219 g         1216 t
ORIGIN      
        1 atatagagga agccgaaagg gcatcccagg aagcaaaccg aatgattaag accatttctg
       61 accctgaacc ggaagcgatg actataacct tagattgtca agtaagatta cagctggcag
      121 cggaaaatta cctcgaagca tcaaagttgg ttcgggagca tggtttagat gtacgtgatg
      181 aaatcttata ttttcatgaa ttggagcata tcgttttagc ccgggtgctc atctattcag
      241 gtcgtgaaaa cccggataac gggcatcttt taactgatgc acaggaatta atcgccagaa
      301 tgttagaggt agcaaaaccc gccgggtgta tgagagaagt gactgtccta cttgtacttc
      361 aggcgctggc ttatcaggcc caaatgaatt acgatctagc gttgagctca ttggcagagg
      421 cgctggtgct ggctgagcaa gaaggctacg tacgtacatt tatcgacgag ggcgatcaaa
      481 tgcaagaatt gctacgcctg gcatcgtccc agggcatcgc cacggattat attggcaaat
      541 tgttgaccgc atttgggtcg ggcaggcttg tcacgcagca aatagcctca ccactcatcg
      601 agccgcttag cgagcgcgaa cttgaagttc tccacctgct cgcaaccgaa ctctccggtc
      661 cggagattgc gcgagagttg gtgatctccc taaataccat gcgtacacac acgaagaaca
      721 tttactcgaa attaaatgta aacagccgag tggcagctat aaataaggca agagagctaa
      781 acctgttata agctacagcg atcccccata tcacgcaaac aaggaagaat tttaaaaatt
      841 gcccctcggg gcttttttga ttcaccattg ccacatcatt tttacttacc agaaatcacc
      901 aggttaatca catcaagtgg tgatgacgcc tcaccacatt tttttgtata ttctgagcaa
      961 ctcatgaatg taaggaaata gataaaagtg ccagaatttt acaaaattct cataaaagga
     1021 cacctgcaag atcgctggga ggaatggctc gaagggctga tgatttgccg gaacgcagac
     1081 ggaacaacaa ccctatctgg cgtactctcc gatcaagctg ctttacacgg ggtgttactc
     1141 aaaatcggta atatgaacct caaattgatc tcagtcaatc caattgaaga gtacctagaa
     1201 gataaaaata caaacagaaa ggagaagtaa aaatgatcac agaacaattt tttagcaaga
     1261 ttgagaggcg ctccatggct gaaagcctat attgggctgg cgtattggta tgggcaggcc
     1321 tggtatttgg ggcaaacagc ttgggcgtcc tgcctcaatt cggcagtggt gatgcctgga
     1381 gctgggtgtt cattggtgcg ggtttgtacg ggacatttat gaatatctac cactccacct
     1441 taccagaccc tgtgacaact acttgggatt acatctggtc cggattttgg ctgttactcg
     1501 gtctgagtgg acttttcgcc atcgatatgt tttggccgct cgtgctggta ttgatcggcg
     1561 gagtgacctt gatcaaggcc attagcaacg gttaggaatc cctctatgaa gcgcattgct
     1621 tggggttttg cttccgggca gtgcgtttca tcagagcgag aattattttt acataccaga
     1681 aatcaagata ggagagcaag aaatgacatt taaacaaaaa acaactcaag aaaagcaaac
     1741 cccatttgac atatctgcct gcatggcaat gatggagaag atgatgggag agcacatggc
     1801 aggatgtgat tgtgctgaaa tgatgtctca aatcacaggc caggcagaag ttccagatga
     1861 atggctgaag gtgatgtccc aaatgatgga acttcattgc cagccgctgg aaggagcgga
     1921 taagcgagcg caagtattat aagatttcgc ttattgacgc tatcgatgtg ccggagaccc
     1981 ctcctaagag accaggagct ccggtgcaac taactaaaca gaggagatat gtgaaatgaa
     2041 tttcaaaacg tctattcaaa ataagtgggg gaggttagct atctttgtcc ctgtcatggt
     2101 tgggttcctg accttggctt ttgcaggctc tgcccaggct gctgaggttg tcacgggcga
     2161 tccggatgca atcgtagccg cgggtgttgt cattaacgac gacctgatta tttctggaga
     2221 caagttggta attgatggca ccatcaccgg caacctgttt gcgaccggaa ttgaggttgt
     2281 cctgaacggc agcgtagaag gcagcgtttt cattggaggc gagcttctaa ccatcaatgg
     2341 agatattggc ggcagcctgt atagcggtgg atgtgcgatc acgcttggac cagaggccac
     2401 gatcggtcgc agtgtttacg ctggtggttt cagcctgatc gccgagagtg gcagtcatgt
     2461 gggctatgat gttcatgctg cggttttcca aaacaggctg aatggggagg tcgggaaaga
     2521 tgttgaggtt gccaccggtg ccttcgaatt caacgggtca gccggcggga atgtggacgt
     2581 cgaattgggc gagacctgcc catattgcct cgaatttgtt cctgtagaag ccattcaacc
     2641 cggtctgagg atcgcagatg attctacggt tgggggagag atcacctaca aagtgaccgc
     2701 gaggaattac gagagtgtgg atgccagtaa cattgaaatc caacccatgg aggttgattt
     2761 cgaagaactt cgaaattctg ttcttcgttt tattgtcgtt caagaaatac gacgggcgat
     2821 aggtgaattt atttccttgc taattgtggg tggactgctc gtttatttcc ttaaagatag
     2881 ggttcggagt gttatggata agatctctgg tagcgcattg cccagctttg gatgggggct
     2941 actggtgatc ttgctcttcc ctctagtgct tatggtcaca ctcgctctag ttattgcgat
     3001 ggctttcata actggtctaa ttactttggg acaactttct agcattgtta tcggcttcgg
     3061 cggttttacc atagcttcct ttgtggtttt tttcgttttc gtgatttcgc tggtaactaa
     3121 agcggtcttt gcttacatgc ttggcaggct tgtagtgggg cgaatttcca aggacacact
     3181 tgagagccgt tggggtgatg tgtgggcatt ggctatcggc gtactgatat acgaaatcgt
     3241 acgcataata ccgatcatcg gcctttttgt agcaatggca gtcatcttgg ttggtgccgg
     3301 agcgattgtg agatctcttc gagatacgct caggctgtcc gcagccattg catgatgtcc
     3361 ttgtgtgtaa tccaggtatg gcttcaacac catacctgga taccagttta ataaaagtaa
     3421 gcaatgctag agactgaaag acttagcaga tgagcgtact aaaaaatgat gtcgccgctt
     3481 tcatcgactt agacaatacg ctttatacga aattcctttg gcaggcactt tttgcacacc
     3541 atagacgaaa cagattcaaa cgccttgttg tggctaggtt tgtggctttt cattttcccc
     3601 tgtggcagtt ggctaaggca ggtctagtac ctaaggacct tttctaccgg ataaatgcaa
     3661 ccaaccttgc ctggctggtg ggaggagttt caatcgaaag ggcggagagg atttgggatt
     3721 ggattatcga tcacgagatc ttacctaatc tgcgccccga gatgcaagct gttatcgagg
     3781 atcaccggaa ccaggtccac agggtcatct tgatttcggg ttcttttaca ccccttctcg
     3841 acaaactagc catccgtttg gggcttgaat gcgctattgc caccccactg gtcgtgaagg
     3901 atggacatta cacgggaaag atcgtccctc ccctgaatat tgggcaaggt aaagttgagc
     3961 gcctccagcg ctttctgaac gaccgaggaa atgaaattga tctggagaag tcttactttt
     4021 ataccgatgc cttcgtggat gcacctgtcc tcgagatgtt cggcaatccg atcgctgtat
     4081 atccggattc acatctggct gccctagcag cagagcgtgg ctggtcggtg attggatgtg
     4141 cccatgtgaa ctgacactta gccttagaaa ggaaacgctt ttcgggaaaa gtgagggggc
     4201 atcttgaata taaacgaaag aaaggtagag tgaaatgaat gcaataatca cacttaatca
     4261 ccataccaat caccaaatga tgtgatgaca catcaccaca ttaatttgta tagttataac
     4321 aagccaatcg aaactgaaat agaaaggaga gaaaaatgac gattgaacag aacacaagta
     4381 agaaaaaaga acgacaacgt ctggaagggc tgtggtgggc agtagctctg atctgggccg
     4441 ggttggtcct tggcgctgac agcatggggt tcctgccgca aattggtagc gcggatacct
     4501 ggagctggat cttccttgga gcgggagtgt ttggctctct tggtaatctt taccggatgg
     4561 gctcatctga tgctccgaac ccaacctctt gggactggat ttggagtggc gccttcctga
     4621 tcctcggcct gggcgggttt g
//
LOCUS       DBNHOH010000288         1510 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1490456, whole genome shotgun sequence.
ACCESSION   DBNHOH010000288 DBNHOH010000000
VERSION     DBNHOH010000288.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1490456"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            367..>1510
                     /locus_tag="AC16V7_05125"
     CDS             367..>1510
                     /locus_tag="AC16V7_05125"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR6256536.1"
                     /translation="MSKIADLEIGLHRRSPEAYAVEFRFSQPDSDVDIRLGQGNVQFD
                     FQTLTQLLPNQPEYGQALTKSLFAESAVQSAFAQALTNIQTLDIPLRIRLFIGPSAPE
                     LHGLYWETLRNPQDDTPLATNENILFSRYLSSLDWRVARLLPRGDLRALVVVANPSDL
                     GEYNLATIDVEGELARAGQGLGQIPVTPLPEPGGDRRATAKDLFELLHTSAYDILYLV
                     CHGALVKGEPWLWLEDEHGKVERLSGGELVTRMKELEQRPRLVVLASCESAGDGSGGA
                     LAALGPRLAESGIPAILAMQGKISMQTVSEFMPLFFSELELDGRIDRALTLARSAVRH
                     RTDFWMPALFMRLKSGRIWYVPGFRDMQDVFKKWQSIAGFIQEKTCT"
BASE COUNT          352 a          385 c          429 g          344 t
ORIGIN      
        1 agggcaatcg catataaccg atttgccccc ttaatggagg taatgggagt gttggagtgg
       61 ggcacagccc tacaccaaca ccccctatcc ccgcctggcg attgaatctg gcttaagatg
      121 ccagttgcag tcgcctgcct gtaaaatgca gactaaaatc gtggtaatat tagcgcgcaa
      181 gtagattgca ggcatatctt cgattcaagt tcctgctcac ccctttcaac gttatcaaga
      241 ttgggcgaaa atccaaactc acttcaaatt tgtatagtaa accagcgtgt taaccaaagc
      301 aatatttacg gaaaggggat ttacgctttg gatggatgat cgataataaa ggatggttca
      361 aagcctatga gcaagatcgc tgacttggaa atcggcttac ataggcgaag ccctgaagct
      421 tatgcggtgg aattccgctt ctcgcaaccg gatagtgacg tcgacatccg gttggggcag
      481 ggcaacgtcc aatttgattt tcaaacattg actcaactgc tgcccaatca gcctgaatat
      541 gggcaagcct taaccaagag cctttttgcc gagagcgccg tacaaagtgc tttcgcccag
      601 gcgctgacca acatccagac tctggatatc ccgctgcgta tccgcttgtt tatcggtccc
      661 agcgcgccgg aactgcacgg cttatactgg gagactctgc gcaatcccca ggatgacacg
      721 ccgctcgcca ccaatgagaa cattttattt tcgcgttatt tgagtagcct ggactggcgg
      781 gtggcgcgcc tgcttccccg gggcgatcta cgcgctttgg ttgtggtagc caacccttcc
      841 gatctgggcg aatacaatct ggcaactatt gacgtggagg gtgaactggc acgggcgggg
      901 caaggtctcg ggcaaatccc ggtgaccccc ttgccagagc ccggcggcga tcggcgggcg
      961 acagcaaagg atctatttga gctgctgcac accagcgcgt acgatatctt gtacctggtc
     1021 tgtcatggag ctttggtcaa gggtgaaccc tggctgtggc tcgaggacga gcatggtaaa
     1081 gtagagcgtt tgtccggagg agaactggta acccgaatga aagagctgga acaacgcccg
     1141 aggctggtag tgctggcgtc ctgtgaaagc gccggagatg gttccggcgg agcattggct
     1201 gctctaggcc cgcggctggc agagagcggc attccggcta tcctggcgat gcagggcaag
     1261 atcagcatgc aaaccgtctc tgagttcatg ccactatttt tcagcgaatt ggagcttgac
     1321 gggagaatcg accgggcgct gacattagct cgcagtgcag tacgccaccg gacggatttc
     1381 tggatgccag ctttgttcat gcgcttaaag agcgggcgca tctggtatgt ccctggattt
     1441 cgtgatatgc aggatgtatt caagaaatgg cagagcatcg ccggctttat ccaggagaag
     1501 acctgcacgc
//
LOCUS       DBNHOH010000289         3118 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1106287, whole genome shotgun sequence.
ACCESSION   DBNHOH010000289 DBNHOH010000000
VERSION     DBNHOH010000289.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1106287"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="AC16V7_05130"
     CDS             <1..454
                     /locus_tag="AC16V7_05130"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6256537.1"
                     /translation="QRALRGQAGAPVISKVDQASLHFLLGRLLRSTGQLDQSLHHLSE
                     AIHLAPDQVNGYLEMGHVHLERREHTQALSAYRQAITVSPGDYRAYYSIGLALKESRD
                     YVGAEKMLRRAAELAPDDVSIHRLLGAVVALNLVHNRREPTREASRPM"
     gene            489..>3118
                     /locus_tag="AC16V7_05135"
     CDS             489..>3118
                     /locus_tag="AC16V7_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6256538.1"
                     /translation="MTSSQNLPSSIHPMSRHLLVRVVQAAIQLGHTRFARQACLNWLA
                     AYPGDLPVNLLYANVLLQDGRNEQAIPILARLCQVDPEYLDAVQAYLRAQKGLHNPGE
                     AQSKNARRRRQRLQNSTNDILNWAMALGINPRAGKATTLPTSSQEASTPIWSEQVLQA
                     RRALEASIASDDKNHELDEAEIALTPALANSSASPLVGVTQLRVLKAKNNPTQSIRGL
                     AEYYLKQWPECVQFQLFLAETLMETGQEEEAVALLHRAAARDLTGQVAIRLWGRGHPY
                     RSLWPERLEHALDIPIPAQVAAALGWNQLPVGEPIAIPLKRDEARPVNHPVPKGVEKP
                     PLTIDNHASSETSATESAGSPKPVFPTIPETLRSVQVELEKVAQRLQQPELARADGRF
                     PVYVIMTTQKGLHTQYGEKASVLTAEMQRLAQAVGARRDWRSVLFFADEGLPPDVRSA
                     RPSDPWAIKLALADLDAHLGCKGEMIGAVLIVGGPEVVPFHHLPNPVDDADDYVPSDN
                     PYATRDENYFIPEWPVGRLPGGVAKDPSGLQILLKSLTDQHIALAKHSKPRNSPWWIA
                     WLNWLLRRQAEPFNSKHKRPSFGYTAEVWRKASQIVFRPIGEAKALRVSPPLSASGQS
                     MVPLPSGKLAYFNLHGLVDAVEWYGQSEAVTSSGNGSGVPNEDQRDYPIAIRPQDIVN
                     SGHAPQVVFSEACYGAHILGKSIEEALALKFLQAGSQAMVGSTCTAYGSINTPLTAAD
                     FLGHAFWSALKQGSPAGEALQRAKVSLAREMHRRQGYLDGEDQKTLISFVLYGDPLAQ
                     PLGQVRQAKGKFRPAVLRPLKPPRGVKTICDRVRLEDQENPLPVEVVDYVKSIVEQYL
                     PGMKGAQVSFSQEHIDCNMDG"
BASE COUNT          773 a          864 c          844 g          637 t
ORIGIN      
        1 tcaacgcgcg ctgcgcggcc aagctggagc accagtgatc tcgaaggtcg atcaagcaag
       61 tttgcacttc ctgctcggca gattgttgcg cagcaccggg cagcttgacc agtctttgca
      121 ccatctaagc gaggcaatcc atttagcacc cgatcaagta aacggctacc tagagatggg
      181 gcacgtccac ctggagcgcc gcgagcacac ccaagcatta agcgcctacc ggcaggcaat
      241 cactgtgtca ccaggagact atcgggccta ctactcgatt ggtctggcgc tcaaagagag
      301 cagggattac gttggtgcag aaaaaatgct gcgccgcgca gcagagctgg cgcccgacga
      361 tgtcagcata catcgtctat taggggctgt cgtagcgctc aatctggtac acaatcgccg
      421 tgagccaacc cgtgaagcca gccgtccaat gtaagccagc ctatttagat gttggttgaa
      481 atacaccgat gacatcatcg caaaatctac cttcttctat tcatcccatg tcacgccacc
      541 tgttggtcag ggtggtccag gctgcaattc agctagggca cacacgtttt gcacggcaag
      601 cttgtctaaa ctggctggcg gcttacccag gggatctgcc cgtcaatttg ctgtacgcca
      661 atgtcctgct acaagatggt cgtaacgagc aagccatacc gattttagcg cggttgtgcc
      721 aggtcgatcc agaatatctg gatgccgtac aggcatactt gagagcccaa aaggggctgc
      781 acaacccggg agaggcacaa tccaaaaatg cgcgacggcg gcgccaaagg ttacaaaact
      841 ccaccaatga tatcttgaac tgggcaatgg ctttgggcat caatcctagg gcagggaaag
      901 caacaacact gcccacctct tcccaagaag ccagcacgcc gatctggagc gagcaagtcc
      961 tccaggcacg ccgggcttta gaggcaagca ttgccagcga cgataagaac catgagctgg
     1021 atgaggcaga gatcgccctg acccctgctc ttgcaaacag ctcagcctcc cctttggtgg
     1081 gcgtcacaca attgagggta ttaaaagcca aaaacaaccc aacgcaaagc attcgtggtc
     1141 ttgccgaata ttacctaaag caatggccag agtgcgtgca atttcaatta ttcctggcag
     1201 agactttgat ggaaacaggc caagaggagg aggcagtcgc gctcttgcat cgagcggcag
     1261 cgcgggatct gacgggtcag gtagccattc gcctgtgggg aagaggtcac ccataccggt
     1321 ctttgtggcc tgagcggttg gagcatgcgc tggatatacc tatccctgcc caggtagccg
     1381 ccgcgttagg ctggaaccaa ctacctgtgg gcgaacctat cgccatccct ctcaagagag
     1441 acgaagcgcg gccagttaac cacccagtcc caaaaggtgt agaaaaacca cctttgacga
     1501 ttgataacca tgcttcatcg gagacgagcg ctacagagtc tgcaggaagt ccaaaaccag
     1561 tttttcctac cattccagag actttaagat ctgttcaggt agagctggag aaggtggctc
     1621 agaggctaca acagccagag ctggcgcgcg ctgatggacg ctttccagta tacgtgatca
     1681 tgaccacgca gaagggattg cacacccaat acggagaaaa ggcttctgtc cttacagctg
     1741 aaatgcagcg actagcccag gcggtggggg cgcgtcgaga ctggcggtct gtgctctttt
     1801 ttgccgatga aggtctgcca cccgacgtac gatctgctcg cccatccgac ccttgggcga
     1861 tcaaactagc cttggcagac ttagatgccc acttgggatg taaaggcgag atgatcggtg
     1921 ccgtgctaat tgtgggtgga ccggaggtcg tcccattcca ccatcttcca aatccggtgg
     1981 acgatgctga tgattacgtg ccctcagata atccctacgc cacacgtgat gaaaactact
     2041 tcatccctga atggcctgtt gggcgtcttc caggcggcgt tgcaaaagac cccagtggat
     2101 tgcagatcct attaaaatcg ctcactgatc agcacattgc cttagcaaaa cacagcaagc
     2161 ccaggaacag tccttggtgg atcgcttggt tgaactggct actacgcagg caagcggaac
     2221 ctttcaactc caagcataag cgccccagct ttggctatac tgctgaggtt tggcgcaagg
     2281 cttcgcagat cgtttttcga cctattgggg aagctaaagc tctacgagtt tcaccccccc
     2341 tcagcgcaag cggacaatct atggtgcctc ttccctctgg caagttagct tacttcaacc
     2401 tgcatggtct ggtagatgca gtggagtggt acgggcaaag tgaagcagtc acatcttctg
     2461 ggaacggatc gggcgtaccg aacgaagacc aacgcgacta cccaatcgcc attcgcccac
     2521 aagacattgt caacagcggg cacgcacctc aagtggtctt cagcgaagct tgctatggag
     2581 cgcatatatt ggggaagagc attgaagagg ccttggcact caagttttta caagcgggta
     2641 gccaggcgat ggttggctcc acatgcacag cctatggatc gattaacacc cccctaactg
     2701 ccgccgactt cctgggtcac gccttctgga gcgccctcaa acagggcagc ccagccgggg
     2761 aggctctgca acgggcgaag gtctccttgg cacgcgagat gcaccgccgg caaggttatt
     2821 tagacggcga agatcagaaa accttgatct ccttcgtgct gtacggagac ccattagccc
     2881 aacctttagg gcaggtacgg caggcgaagg gaaagttccg cccagcggtg ctgcgcccgc
     2941 tgaaaccgcc acgcggcgta aagactatct gcgatcgagt gcgattagag gatcaggaaa
     3001 accccctgcc ggttgaggtg gtggattacg tcaaatccat cgtcgaacaa tacttacccg
     3061 gcatgaaggg agcgcaagta tcttttagcc aagaacatat agattgcaac atggatgg
//
LOCUS       DBNHOH010000290         3343 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_722154, whole genome shotgun sequence.
ACCESSION   DBNHOH010000290 DBNHOH010000000
VERSION     DBNHOH010000290.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_722154"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /locus_tag="AC16V7_05140"
     CDS             complement(<1..1274)
                     /locus_tag="AC16V7_05140"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6256539.1"
                     /translation="MVSVGRHPNITLLSYSEVEAVSGYVGNFNIVVRRKPRYIDETQC
                     TGCGKCAEVCPIEIKNPFDLGLSTRKASYRFSAQSVPGAYTIQKSGIAPCRSACPTDQ
                     RAQGYIALLRQKRYADAYWAIRREHPFPSVCGRVCNHRCEDACSRAKYDEPVNIMGLK
                     RFIADWAFHHRDQLPSMRDKSLAGTPFQHHHPPTGKKIAIIGAGPAGLTAALDLVRLG
                     HAITIFDALPVAGGMMRVGIPPHRLPVEMLDWEIQQIIDEGIDLQLNTWVDDIPALLD
                     NGYNAVLIATGAHQAKKLPIRNSNHPGNWLSLKLLRSVALGENINLTGKKVVVLGGGN
                     VALDTARTVLRLGASQVRMACLEPRGEMPGFEWEVKVAEDEGVILCPARTFKEIVVEN
                     EKISGVRCAEVIFHGFKRGRPDIEEISSTEHI"
     gene            complement(1290..1883)
                     /locus_tag="AC16V7_05145"
     CDS             complement(1290..1883)
                     /locus_tag="AC16V7_05145"
                     /inference="COORDINATES: protein motif:HMM:NF013086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6256540.1"
                     /translation="MNRSKIRTGVYVCHCGTNIAANVDVKSVAEYAQELDGVILARDY
                     TYMCSDPGQDLIKQDIQERNLNRIVVASCSPLMHEPTFRRACQDAGLNPFFFQMANIR
                     EHCSWVIDDRQLATNKAKSMVEAAVRRVRYHTPLSIRNVDMTPSALIVGGGIAGIEAA
                     LRLADAGKQVYLVERESSIGGHMAQLYKTFPTLDCAA"
     gene            complement(1880..3022)
                     /locus_tag="AC16V7_05150"
     CDS             complement(1880..3022)
                     /locus_tag="AC16V7_05150"
                     /inference="COORDINATES: protein motif:HMM:NF024247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR6256541.1"
                     /translation="MPVIDTQNCAYFLRGKCRACEKFCQREAINFEQQDQLIDVQVGT
                     IILATGFKDFDPRQSPKYEFSNLDNVVTGMELERLINTGGPTTGNVLLKDGRKPKSIG
                     LLHCVGSRDPEHHEYCSRVCCMYSLKLAQLIRHYIDAEVYEFYRDMRTFGKDYEAFYE
                     QVRRDGVQFIRFDEELKISQRDGQIQISAIDVYTGSPNSYLVDMLVLSTGLEPNADQL
                     RVAKTFGVSLSPDGFFLEKHPKLAPVETATDGIFLAGTCQAPKDIPDSVAQGGAAAAA
                     ALSLMDAGHVSLEPFTVHIIPENCGGCRTCEGLCPYGAIQMVDNAERQVAYVNEVLCK
                     GCGACAAACPAGAALQNGFTYEQILAEIEGALLESLQIYPVQEMPV"
     gene            complement(3104..3271)
                     /locus_tag="AC16V7_05155"
                     /pseudo
     CDS             complement(3104..3271)
                     /locus_tag="AC16V7_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012267.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
BASE COUNT          782 a          849 c          825 g          887 t
ORIGIN      
        1 aagatatgtt cggtgctaga aatctcctcg atgtccggtc gcccacgttt aaagccgtgg
       61 aatatcactt ctgcacagcg tacaccagat attttttcat tttctacgac gatctctttg
      121 aatgttctgg cggggcataa aatcacacct tcgtcttcag ccacttttac ttcccattcg
      181 aagccaggca tctcaccgcg cggttccagg catgccatgc gaacctggct ggctcccaag
      241 cgcagcacag tgcgtgcggt atccagggcg acgtttcccc cacctaagac aaccactttc
      301 ttgccggtta gattaatatt ctcgccaagg gcaacgcttc gaagcaattt caggctgagc
      361 cagttgccgg gatggttgga gtttcgtata ggtagctttt tggcttggtg tgcaccagta
      421 gcaatcaaaa ccgcattgta gccgttatcc agcaaagcag gtatgtcatc gacccaggta
      481 ttaagttgca aatcaatgcc ttcatctatg atttgctgga tttcccaatc gagcatctct
      541 actggtaacc tgtggggagg gatgcccacc cgcatcatgc cacctgctac tggtagggca
      601 tcaaagatcg tgatggcatg gcctaaacga actagatcca gcgcagcggt taatcctgct
      661 ggaccggcgc caattattgc tattttcttt ccagtaggcg gatgatgatg ttgaaatggt
      721 gtgcctgcaa gacttttgtc gcgcatactt ggaagttggt ctcgatggtg aaaagcccaa
      781 tcggcaataa atcgttttaa acccataata ttgactggct catcgtactt ggcacggctg
      841 cacgcatctt cgcaacgatg attgcacact cgtccgcaaa cggatggaaa tgggtgttcg
      901 cgacgaatag cccagtaggc atctgcatat cgcttctgac ggaggagggc gatatatcct
      961 tgagcgcgct ggtctgttgg gcaggcgctt cggcagggtg caatacctga tttctgaatt
     1021 gtgtaagccc ctgggaccga ctgtgctgaa aaacgatagg atgctttgcg cgtgcttaaa
     1081 cccaaatcga atgggttctt gatctcgatt gggcagacct cggcgcactt gccacagcca
     1141 gtacactggg tttcatcaat gtagcgtggt tttcggcgaa caacaatgtt gaaattgccc
     1201 acatagccgc taactgcctc aacctcgctg tagctaagca gtgttatgtt tgggtggcgt
     1261 ccaacggaca ccattttagg ggtgagaatt caagccgcac aatctagggt agggaatgtc
     1321 ttgtaaagtt gggccatgtg cccaccgata cttgattccc tctcaacgag atatacttgc
     1381 ttgccggcgt ctgccagtcg aagtgcagct tcaataccag ctatgccacc acctactatt
     1441 agagccgatg gcgtcatatc cacgtttcga atcgataatg gagtgtgata gcgcactctt
     1501 cgaacagctg cttccaccat acttttggct ttgttcgtcg ccaattgacg gtcgtcgatc
     1561 acccaggaac aatgttcgcg gatatttgcc atctggaaaa agaatggatt taaacccgca
     1621 tcttggcatg cgcggcgaaa ggttggttca tgcatgagtg gtgaacatga agccaccacg
     1681 atccggttaa gattgcgctc ttgaatatct tgtttgatta aatcctggcc tggatctgaa
     1741 cacatgtacg tgtaatctct ggcaaggatg acaccatcga gttcctgtgc atactcagca
     1801 accgatttta cgtccacatt ggcagcgata ttagtaccgc agtgacaaac gtataccccg
     1861 gttctgattt tcgatcggtt catactggca tctcctgtac aggatagatt tgcaaggatt
     1921 cgagcaaggc gccttcaatt tcagctaaaa tttgctcgta ggtgaatcca ttctggagtg
     1981 ctgctcctgc agggcaggct gctgcacaag cgccacaccc cttgcagagc acttcgttga
     2041 cgtatgcgac ttgcctttca gcgttatcga ccatttgtat agctccatac ggacatagcc
     2101 cttcgcaggt gcggcaacct ccacaatttt cgggaataat atgcactgtg aacggctcca
     2161 gggagacgtg accagcgtcc attaaagaaa gcgccgccgc ggctgctgcg ccaccctgag
     2221 caacgctgtc cgggatatct ttgggtgcct ggcaagtgcc tgctaaaaag atgccatccg
     2281 tggctgtctc caccggcgcc agcttgggat gtttctcaag gaagaagcca tctgggctta
     2341 gactgacgcc gaatgttttg gcgacccgca actggtcggc gttgggctcc aggccggtac
     2401 ttaaaaccag catgtctacc aagtagctgt tggggcttcc cgtgtaaaca tctatggcgg
     2461 agatttgtat ttgcccatct cgttggctga tcttaagctc ttcatcaaaa cggatgaact
     2521 gtacgccatc gcgtcgaacc tgctcataaa aagcctcgta atctttgcca aaggtgcgca
     2581 tatcgcggta gaactcatat acttcagcgt ctatataatg acgaataagc tgggcaagct
     2641 tgagagagta catacaacat acccttgaac aatattcatg gtgctcaggg tcgcggctgc
     2701 ctacgcaatg taataatcca atgctttttg gcttgcgtcc gtcctttagc agcacatttc
     2761 cagtagttgg gcctccggta ttgatcaggc gctcaagttc cataccggtc actacattgt
     2821 ctaggttgct aaactcatac ttcggtgact ggcgtggatc gaagtcttta aaaccggtcg
     2881 caaggataat tgtccctacc tgaacatcaa tcaattgatc ttgttgctca aaattgatgg
     2941 cttctctttg gcaaaacttt tcgcaggcgc ggcattttcc ccgcagaaaa tatgcgcagt
     3001 tctgcgtgtc gatcacaggc acgcgcggta ctgcttgggc aaaaggaaaa tatatcgctg
     3061 ggcgagtgcc taatcctaga tcgaattctg aggggatttt tttccaggga catttctcta
     3121 cgcacgtacc gcatccggta caattttcct cactcacgta acgggctttc tttctcacgc
     3181 ttactgtgaa gttgcctaca tagcccgaaa ctgattcgac ctcgctgttg cttagtagtc
     3241 tgatgttttc gtggcgtcca acggacacca ttttgggggt gagaattcaa gccgcgcagt
     3301 ccaatgtcgg gaacgttttg tagagttgag ccatatgtcc acc
//
LOCUS       DBNHOH010000291         1679 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1684096, whole genome shotgun sequence.
ACCESSION   DBNHOH010000291 DBNHOH010000000
VERSION     DBNHOH010000291.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1684096"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="AC16V7_05160"
     CDS             <1..817
                     /locus_tag="AC16V7_05160"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010505912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR6256542.1"
                     /translation="EATALAERAPQSYSSVVGVGGDGTLHEIVNGLLRASGEAETISV
                     GIVPLGNGDDFAKMIPPETPVGGKPFDWQNAVTKIARGKTHLFDVGRISSNPPPSKAH
                     NRPHYFINSLDVGFGAQGSHNFRTIPKFLSGLPAYLAAVLKTLINYSIPNVRIQLDDQ
                     PAFEQSTTITAVMNGRCFGNGFWVCPEARADDGFLDVMVAQALGRASILRLIPKIMKG
                     THVNEPVLSMHRARRVEIESQEPLLVEADGEFPSLGTHHLKIEILHKKLRMIV"
     gene            944..1243
                     /locus_tag="AC16V7_05165"
     CDS             944..1243
                     /locus_tag="AC16V7_05165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HIR6256543.1"
                     /translation="MYGTVARLRVKPGAEKQLIEMSQNDMDSQMRGYVGQYVYRMDAD
                     PNEYYLVVIFESKEAYFANADSPEQDARYRAFRALLEQDPEWHDGEIVYSAGQVK"
     gene            1249..>1679
                     /locus_tag="AC16V7_05170"
     CDS             1249..>1679
                     /locus_tag="AC16V7_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013507.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Kelch repeat-containing protein"
                     /protein_id="HIR6256544.1"
                     /translation="MKVTRIILWGILIVLVVPIGAYLASTSVVCRVPAYPLSPDAGRW
                     AEKANLPTPRSELAATAAGGKIYVAGGLEGSATSDAFEVYDPASDTWKAAKNLPVALH
                     HTALAATGGKVYMTGGFGDLAFSQTSNLAWVYDPQSDNWQQV"
BASE COUNT          410 a          454 c          454 g          361 t
ORIGIN      
        1 cgaagcgacc gcgctcgctg aacgagctcc gcaaagctac tcaagcgtgg ttggagtagg
       61 cggcgatggc accctgcacg agattgtcaa tgggctgctg cgcgcttcgg gcgaggcgga
      121 aaccatctcc gtgggcattg tcccgctggg caacggcgac gactttgcca aaatgatccc
      181 gcctgagacc ccggttggag gtaaaccctt tgattggcag aatgcagtga caaaaatcgc
      241 tcgcggtaaa acccacttat tcgatgtagg gcgcatttcc agcaacccac cacccagcaa
      301 ggcacacaat agaccacatt atttcatcaa cagcctggat gtaggcttcg gtgcccaagg
      361 ttctcacaat ttcaggacga taccaaaatt tctgagcggc ctgcccgcct atcttgccgc
      421 agtgcttaag acgttgatca attactccat ccccaatgtt cgcattcaat tagatgacca
      481 acccgctttc gaacaatcca caaccattac tgcggtaatg aacgggcgct gttttggaaa
      541 cggattttgg gtttgccctg aggcgcgcgc agatgacggc tttttagatg taatggtggc
      601 tcaggcttta gggcgcgcca gcatcctcag gctgattccg aaaatcatga aggggacgca
      661 tgttaacgag ccggtcttaa gcatgcatcg cgcccggcgc gtggagatcg agtcgcaaga
      721 acccctgctg gtcgaagccg atggcgagtt tccttccctc ggaacacatc atttgaaaat
      781 cgaaatactt cataagaagt tacgcatgat cgtatagcac tcaaccaagc taaagaaaaa
      841 ttcggattgc actcatctga attgcaattt gccggttctt ggcttacaat gggggatgtc
      901 aagatccaat tacggccata ttcaccaggc aaaggaggaa cctatgtacg gaaccgtggc
      961 gcggttgcgg gtaaagccgg gcgcagaaaa acagcttatc gagatgagcc aaaacgatat
     1021 ggattcacag atgcggggct atgttggtca gtacgtctat cgcatggatg cagatcccaa
     1081 tgaatattat ctggtggtga tcttcgagag caaggaggct tacttcgcta acgcagatag
     1141 cccagagcaa gatgcacgct accgcgcctt tcgtgctctg ctcgagcagg atcccgagtg
     1201 gcacgacgga gagatcgtct actcagcggg tcaagtcaaa taagtcactt gaaagttacc
     1261 cgcatcatct tatggggtat attgatcgtg ctggtagttc ccatcggagc ttatctcgcc
     1321 agcacatctg tggtgtgccg cgtccctgcc tacccattat ctccagacgc aggccgctgg
     1381 gctgaaaagg cgaacctccc cacgccccgc agcgagctgg ctgccaccgc ggcgggtggc
     1441 aagatctacg tcgccggtgg gttggaagga tccgcaacat ccgacgcttt tgaggtctac
     1501 gatcccgcaa gcgacacctg gaaggccgcg aaaaacctgc cggtggcatt gcaccataca
     1561 gccctggcag ccactggtgg caaagtctac atgaccggag gctttggcga tttggccttc
     1621 agccaaacca gcaatttggc ctgggtttac gatccgcaaa gcgataactg gcagcaggt
//
LOCUS       DBNHOH010000292         1846 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_657939, whole genome shotgun sequence.
ACCESSION   DBNHOH010000292 DBNHOH010000000
VERSION     DBNHOH010000292.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_657939"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..338)
                     /locus_tag="AC16V7_05175"
     CDS             complement(33..338)
                     /locus_tag="AC16V7_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256545.1"
                     /translation="MAQINLSWLLSLSLSAKNWAECKVQSEHRSAQSSALVSALRLLS
                     SSAVPSARVLGVYHAFLSALFKGDISTLSNMGHFYFGLTDTNFPERYFLEAQPVDNS"
     gene            complement(325..1605)
                     /locus_tag="AC16V7_05180"
     CDS             complement(325..1605)
                     /locus_tag="AC16V7_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_246585292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HIR6256546.1"
                     /translation="MSKKELSRLEIMQRIQEKRLTQKEAAWMLGLSVRQVKRLYRAYR
                     QQGAAGLVSRRRGKPSNNRMDPQVEQQVIDLIHERYRDFGPTLAHEKLVELHGLKISD
                     ESVRKLMIAERLWKPKKAKRSAVHPLRERRACLGELVQIDGSEHAWFEARGPKCVLLV
                     FIDDATGQLGELRFVPQETFFAYCEASRHYFEQHGKPLAFYSDKDSIFRVNQPRPLGL
                     SSGQTQFARAMEELDIQTLCANTPQAKGRIERVIQTLQDRLVKELRLRGISDMQAGNA
                     FLPEFRADFNRRFGVVPRSSHDAHRPLLPSDNLERILTHQEIRTLTKNLTVHYHNVVY
                     QIHTQRPTYALHKAPVTVCENARGEVTLLYKDQPLDYSTYHKPQRQAEVVSSKTLDHQ
                     LRLPKPPPANHPWRRYGRHLNGKPIQKVFPDGAD"
BASE COUNT          383 a          489 c          519 g          455 t
ORIGIN      
        1 ttccccatta agtgcgtttt atccacagtt tttcacgagt tatccacagg ttgagcctct
       61 aaaaagtacc gctccggaaa atttgtgtct gtcaagccaa agtagaaatg tcccatattt
      121 gacaaagtag agatgtcccc tttgaagagg gcagatagaa aggcatggta gacaccaagc
      181 actcgtgcgg agggtacggc actgctggag agcaagcgga gcgctgacac gagtgccgaa
      241 gactgggcag agcggtgctc gctctgcact ttgcattctg cccagttttt ggccgaaagc
      301 gataaactca agagccagct caaattaatc tgcgccatct ggaaagacct tctggatggg
      361 tttgccattg aggtggcggc catagcgacg ccaggggtga ttggcagggg gtggcttagg
      421 taggcgcaac tggtggtcga gggtcttgct ggagaccact tctgcctggc gttggggttt
      481 gtgataggtg gagtaatcga ggggttggtc tttgtagagc agggtgactt ctccacgggc
      541 attttcgcac accgtgaccg gggctttgtg cagggcatag gttgggcgct gggtgtggat
      601 ctgatagacg acattgtggt agtgcacagt caagttcttg gtcagggtac gtatttcctg
      661 gtgggtgagg atgcgctcca ggttatcgga gggcagcaaa ggtcggtggg catcgtggga
      721 actgcgtggg accaccccaa agcggcgatt gaaatctgcc cggaattcag gtagaaaggc
      781 attacctgct tgcatatctg agatgccgcg cagacgcagc tccttgacca ggcgatcctg
      841 cagggtctgg atgacccgtt cgatgcgacc tttggcttgt ggggtgttgg cgcacaaggt
      901 ttggatgtcc agctcttcca tcgcccgtgc aaactgggtt tgcccactgc tcagtcccaa
      961 ggggcggggc tggttgaccc ggaagatgct gtctttgtcg ctgtagaagg ccaacggctt
     1021 gccatgctgc tcaaagtaat ggcgcgaagc ttcacaatag gcaaagaaag tctcctgtgg
     1081 tacaaacctc aactctccca actgcccggt ggcgtcgtcg atgaacacta acaggacaca
     1141 tttcggccca cgcgcttcga accaggcgtg ctccgagcca tcgatctgca ccaactcccc
     1201 caaacaggct cgccgctcac gcagcgggtg cacggctgac cgcttggctt tctttggctt
     1261 ccacagcctt tcagcgatca tcaacttacg cacactctcg tcagatatct tcaacccatg
     1321 caactccacc agtttctcgt gcgccagcgt cggcccaaag tcccgatagc gttcatggat
     1381 caaatcgatg acctgctgct ctacttgggg gtccatacgg ttgttactcg gtttgccacg
     1441 ccgtctgctc accagccccg ctgctccttg ttggcgataa gctcgataca gtcgcttcac
     1501 ctgtcgcacg cttagcccaa gcatccaagc tgcttccttc tgcgtcaaac gtttctcctg
     1561 gattcgttgc atgatttcta atcggctcag ttcttttttg ctcattgtca gtaggttgtc
     1621 cactttgttg tccctttcga aggggacatt ttaactttgg taaaagggga ctctactact
     1681 ttgggctaac atctagcaat tatccctatg caaaccgcac cctttgggtt atactattga
     1741 caggagccac gcagatgcgt ggcttacttt ccgctcctga ccggctccca gccgagttta
     1801 ggggcaaacc cgtggaaagg aggcatcccc tgcatgcgcg agtatg
//
LOCUS       DBNHOH010000293        18665 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_593661, whole genome shotgun sequence.
ACCESSION   DBNHOH010000293 DBNHOH010000000
VERSION     DBNHOH010000293.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 18665)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18665)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18665
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_593661"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1267
                     /locus_tag="AC16V7_05185"
     CDS             <1..1267
                     /locus_tag="AC16V7_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6256547.1"
                     /translation="REEIILRVNQALEYLGISELRERETFHLSGGEKQKVALAGVLAM
                     RPSILVLDEPLASLDPASALETLQLVRRMADEGMTIMMVEHRVEDVLKINPDRAMFMM
                     DGKVHYLGSPEGLATVVDYREVKLPAHMIIERVAAESLALEPQELRGAVDRTTETTPI
                     VQFKDVTFEYESGVDVLHGINLTIRRGDVIAVLGPNGAGKTTLVKHAIGLLKPKSGQV
                     LVNGRDTRELTVAQVASTLGYVFQSPSHMLFAPTVIEELAFGPTNLRHSPDEIEKEVS
                     EAIKIVNLSGREEDPPLALSFGQQKRVSIAAILAMRSRILVMDEPTAGQDYKNYMSFM
                     DSILQLPNFEAILFITHDLDLAVIYANRVLLVSGGQVVDDGPAQEVLADIEWLKTCRL
                     VPTTLLQANLERLHRTGRFLRAEALAQVQ"
     gene            1358..2293
                     /locus_tag="AC16V7_05190"
     CDS             1358..2293
                     /locus_tag="AC16V7_05190"
                     /inference="COORDINATES: protein motif:HMM:NF018816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR6256548.1"
                     /translation="MNKLTSVVIALVITLILFLALVYVGGTILGIERLQTGENPVLRF
                     VFVIIGLLVVFFVYYLTRDNKAWEVGTREVVFMAIGAALYAIFSYLFNGTVFAVPSVS
                     QVALRPAICIPIFFGYTFGPLVGLFTGAVGNMLGDALTGFGLYPQWSLGNGLIGMISG
                     MAWLFTDKKRSLNSVLIVSAVLAVIATLVYYFNRTLPNMLFFDPEQNIFGDQPITLFA
                     GISAVVGFVLILVVRYMFGQSIDVTAAVTWGMLGNLIGLGFASISDIWINGFSLPVAI
                     VGEFLPSAGPNLIFTAILVPLLVGAYAATRRQAGR"
     gene            2360..3229
                     /locus_tag="AC16V7_05195"
     CDS             2360..3229
                     /locus_tag="AC16V7_05195"
                     /inference="COORDINATES: protein motif:HMM:NF014420.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HIR6256549.1"
                     /translation="MLVTWRYRKRKSLIQTFDPRAWLIFFGCFLASTLFFWDLRFLLV
                     MLAIALFFVFTSDIHWHESRRAWLFIGGFILFYAALVFLTGRGGTELYDQEYLLKRLE
                     VPFTIFGWRPGINITAERIMFASSQLVRVFSIAAMTILIPYSLNPAQYGVAFRGLGLP
                     DKIAYAMDLTMRFIPTFGRDFQLTMDAQRARGYEIEKLSGGLFAQVRKLAPLVVPVTI
                     HAIMGSEDIIDAMDLRAFGVGPRMWLEQLHYRKRDRVLIYMGILLFITSLVVTILGYG
                     EFWVPEFWYQLAQ"
     gene            complement(3498..4658)
                     /locus_tag="AC16V7_05200"
     CDS             complement(3498..4658)
                     /locus_tag="AC16V7_05200"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR6256550.1"
                     /translation="MSSVLGLALSERVLALAMTIQQIPAPTFSEARRAAFIFERFQAE
                     GLSDISMDAIGNVYGRLPGAGSTPPLVVSAHLDTVFPEDTPLDCRREAETIYGPGIGD
                     NALGVAGLFGLLWGVKEIVSPRLQPNLSGLKAKSSLALPGDLWLVANVAEEGMGNLLG
                     MTAVVDRFGGDARAYLILEGMALGQVYHRGLNVRRYQISASTAGGHSWVDYGQPSAIH
                     ELAGLITRLSALSLPSQPRTTMNVGVISGGTTVNSIAAEANCLLDLRSEGHESMVNLA
                     TEVEGLIDAGNRNGVQFSWEVVGQRPAGEMPRDHPLVQLAASCLESQGIQPLFNIGST
                     DANLPLSRGLPAICIGLTTGGGAHTTGEYIHTQPLSQGLAQLLMLVEAVFNG"
     gene            complement(4655..5674)
                     /locus_tag="AC16V7_05205"
     CDS             complement(4655..5674)
                     /locus_tag="AC16V7_05205"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR6256551.1"
                     /translation="MESKLPRFIIAGSLLREYIILPSGEALLDVPGGNVLYAAVGLKL
                     WEPEPPPAVLARVGEDYPQEWLDQFTRRGLDTRGVNVISQPVDLRAFTAFLSRTNRAT
                     DDPVAHFARAGLTFPKELLGYRDSSSDLNSRTNLSSISLRQGDIPADYLDASAAHLCP
                     LDYLTHSLLPAVLRQAEFTTVTLDPSPGYMNPTFWDDVPALVTGLTAFLPSEEEMSSL
                     FQGRSTDLWQMAEGLASYGCEIIVIKRGEGGQLLYDAAGRARWEIPAYPSRMVDPSGA
                     GDAFCGGFLAGYRRTYDPLQAALHGNISASLVVEGNGPFYALDCLPALPQARLEALQQ
                     GVRKI"
     gene            complement(5771..6706)
                     /locus_tag="AC16V7_05210"
     CDS             complement(5771..6706)
                     /locus_tag="AC16V7_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6256552.1"
                     /translation="MSIEVIHATNLTRRFGDFTAVDHISFTMNAGEVVGYLGPNGSGK
                     TTTIRMLLGLLIPTEGEASVLGYDVVRQAEQIRERVGYMSQKFALYHDLTVGENLAFY
                     AGVYGERSPARLAEVLELVGLSQLEGERVSDLPVGWRQRLALGTAIVHRPKLLFLDEP
                     TSGVDPTTRRAFWDLIYALVDEGVTALVTTHYMDEAEYCGRVGIMRDGRLLAMDAPST
                     LKTSALPGLTWDVFLDGVSSTGASSLLVALNALETCPCVLRAGLVSDHLRAITPADVS
                     AETLRQHLDEAGIEGLRIDPAEPTLEDVFLALATR"
     gene            complement(6766..7713)
                     /locus_tag="AC16V7_05215"
     CDS             complement(6766..7713)
                     /locus_tag="AC16V7_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6256553.1"
                     /translation="MHKSFGETRAVDGLNLRVAAGEIYGLVGPDGAGKTTTLRLLCAA
                     LQLDRIKDDEQSSIRISGYDAVRQTELVRAQIGYLPQRFSLYEDLTVLENIRFFAEVR
                     GMSSADWRPRCMEILGFVGLDPFVDRLAGHLSGGMKQKLGLAAALVHRPRVLLLDEPT
                     TGVDPVTRQDFWQLIIRLASQANHDHAEVAVLVSTPYMDEAARCTRVGFMRAGRLLIE
                     GSPADLRLRLKDQILELRGQPLDMLRHLAHADQDVEDVQMFGDRIHLRTRLGSRDLVE
                     ERLTQRILAEGGEITLLRPVAPQLEDVFISLLDQPNSSS"
     gene            complement(7779..9125)
                     /locus_tag="AC16V7_05220"
     CDS             complement(7779..9125)
                     /locus_tag="AC16V7_05220"
                     /inference="COORDINATES: protein motif:HMM:NF052764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR6256554.1"
                     /translation="MHPNPRKIVPIVLLIAILGGAIWYFTLGQGNGNSSALTASGTIE
                     GTQVVIAPELGGRVREVMVQEGEVVEAGQTLVRFDDSLLQAQLSQARAALHLTQANYD
                     LAAKGPTDEQRRLAIASAEMELTTARQALQALYDNSGLTESQLEQSIAAIDKSRDKAQ
                     QYLDNVNTEADPSDIDAAWASVVIAQDKLEDALEDFEPYEKKDQSNLTRAIFQAQVAS
                     AQKQYDSMVERYNNLVGTANQYELALARSEVSLAEAKLADAKRQYEELQEGIDPDSLA
                     LAQAHLNTAEANLAVAKADPSPEQLAAAQAQVEVAQGAMEVIEAQIAKLVINAPMDGV
                     VLTRTAEPGEVLVPGAPLLSLLRLDDLTITVYVPEDRYGTIKLGQTASVTVDSYPSEA
                     FEATVIRIADQAEFTPRNVQTEAGRRTTVFAVELSVTNPEGKLKPGMPADVDFSLK"
     gene            complement(9144..10274)
                     /locus_tag="AC16V7_05225"
     CDS             complement(9144..10274)
                     /locus_tag="AC16V7_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6256555.1"
                     /translation="MNSRLTSLIRKEFLQILRDPRTLALALVMPLVQLFLLGYSATND
                     VRNVPLAVYNQDRGPEARALLDAYRAANYFSLEYDVSSEQELRDLIDRGQARGGLVIP
                     PDYSSRLQGDGQAEVAFILDGSDPTVAATALSAAQVIGQQHATSVLSDRLGRRGQSSA
                     IRPPIEVRTQVWYNPDLESAYFMIPGVIGMILYAITSILTSTAIVRERERGTIEQLIV
                     TPIRPWELVVGKMLPYVILAFLNTLEVLAIGALWFHVPIRGDLGLILLLSALFLLTSL
                     GIGLLASTIANTQQEAMLTVWMTLLPSIFLSGFFFPLEAMPKVLQWLSYLMPLRYYLV
                     IIRSLLLKGVGADMLKSDILALAIFGFAIMAAAAMRFRKRLD"
     gene            complement(10271..10897)
                     /locus_tag="AC16V7_05230"
     CDS             complement(10271..10897)
                     /locus_tag="AC16V7_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6256556.1"
                     /translation="MSEEIAPRGERTRNDILQAAYQLFLERGFHGTSMRQIAQAAGIA
                     LGGIYNHFSSKEDIFTAVFLERHPFRDVLPLMNTAQGETVEEFVRDAAQRMVAGLGQR
                     TDFLNLMFIELVEFKGQHVPHIFQIIFPEMMEFTQRFQTERQELRDIPLLVVVRAFIG
                     LFFSLLITEIMIADQLPPEEREHALDYFVDIYLYGILKGGTTLEKVQQ"
     gene            complement(11136..11471)
                     /locus_tag="AC16V7_05235"
     CDS             complement(11136..11471)
                     /locus_tag="AC16V7_05235"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HIR6256557.1"
                     /translation="MEELKEMKCVPCRGGVPPLTSEQIAGLHPGVPEWQVLDVEGIQR
                     LQRVFKFKNFAQALEFTNKVGALAEEEDHHPALLTEWGKVTVTWWTHKINGLHRNDFI
                     MAAKTDALK"
     gene            complement(11556..11786)
                     /locus_tag="AC16V7_05240"
     CDS             complement(11556..11786)
                     /locus_tag="AC16V7_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256558.1"
                     /translation="MSNNGNEQAGGNWKSKALLAGAVLGAVVGLSAAYLIIQRAERED
                     GEVRISPGDGVKLGVLVFGLLRQVAQLGEGEK"
     gene            complement(11802..12233)
                     /locus_tag="AC16V7_05245"
     CDS             complement(11802..12233)
                     /locus_tag="AC16V7_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256559.1"
                     /translation="MNENTFPPSAPKPNPVTQRKHRQEVLWQITLPLALGALLVLAAG
                     AGVVYAGATQTGPVDRWASISIIWLIIPTMFLTLIFLVVTAGLAYGIIWLIGALPKYT
                     RQIQDVFVLIDARVRKAADAIVEPTLRVQSFTAGLRALRRK"
     gene            complement(12241..13167)
                     /gene="prmA"
                     /locus_tag="AC16V7_05250"
     CDS             complement(12241..13167)
                     /gene="prmA"
                     /locus_tag="AC16V7_05250"
                     /inference="COORDINATES: protein motif:HMM:NF018076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR6256560.1"
                     /translation="MGNSSWLEISLIVDGEMAEAVAEVLGRYVSGGVAIESTAVTARA
                     DDSEGQAIGPLRVCGYLPVDDQLDENRQRVEQALWFLGRIRPLPAPQYHIIHEQDWSQ
                     AWKSHYHPIAIGQRLMVVPAWEQPPDPARIAIRMDPGMAFGTGTHPTTQLCLELMEIY
                     LPGREAAIIDLGCGSGILAVAALKLGAEQALGVDIDLEAVQATRDNAELNGVAGQLEI
                     GKGSVDEIRRGEFALRRANLVLANILAPVIIRLLDDGLPDLLTPDGVLILSGILEEQA
                     VEVEDALQRYNLRTLDRRQSGDWVALVAGMIL"
     gene            complement(13252..14382)
                     /gene="dnaJ"
                     /locus_tag="AC16V7_05255"
     CDS             complement(13252..14382)
                     /gene="dnaJ"
                     /locus_tag="AC16V7_05255"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR6256561.1"
                     /translation="MMTERDYYEILGVPKNASNEELKSAFRRLARQFHPDVNKESDAE
                     ERFKEINEAYAVLSDQDKRSVYDRLGHAGVKGAGGVPDFSVDFSDFADIFGDLFGFGG
                     FTRSSRRQRNAPRRGQDLSYRLDLTFEEAVFGLEKEIEFVRDELCNTCSGSGAEPGTS
                     PVRCTTCNGSGEMRQVRQTLLGSMVQVSTCPTCKGVGETITTPCHTCQGRGLERRTRR
                     KVVNIPGGVDDGTQIRLAGEGQPGLNGGPNGNLYITIHVQPHKFFRRRENDIILDLDI
                     NLAQATLGAEVEIPTVDGPARLKIPSGTQPGKVLRMRSKGVPYLRSNGRGDQLVVVNV
                     DIPTRLTSEQRELFEQLAKSLGSEVRPQERSFIDWLKETLGG"
     gene            complement(14459..16363)
                     /gene="dnaK"
                     /locus_tag="AC16V7_05260"
     CDS             complement(14459..16363)
                     /gene="dnaK"
                     /locus_tag="AC16V7_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR6256562.1"
                     /translation="MGKIIGIDLGTTNSVVAVMEGGEPVVIPSAEGERLVPSVVAFNK
                     NRERLIGRVARNQAVVNPENTIFSIKRFMGRKFSDPEVQRATTRVPYKVAQAPNGDVR
                     VILDEREYSPPEVSAMILGKLKADAEAYLGETVTQAVITVPAYFNDAQRNATKDAGKI
                     AGLEVLRIINEPTASSLAYGLDKKKDELIAVYDLGGGTFDISILDVGDGVFQVRATSG
                     DTFLGGDDFDQRIIDFIADEFKREQGIDLRKDRQALQRLKEGAEKAKIELSSMMQTEI
                     NLPYITADASGPKHLVMTLTRAKLEQLTADLIERSLGPVRQAMKDAGLEPDQVNEVVL
                     VGGMTRMPAVQDAVRKLFGKGPHKGVNPDEVVAVGAAIQAGVLGGEVKDILLLDVTPL
                     TLSVETLGSVASPMIERNTTIPTRKSQIFSTAADSQPQVEIHVLQGERPMANDNKSLG
                     KFILDGIPPAPRGIPQIEVTFDIDANGILKVTAQDKATSRSQNITITASSGLAEAEVE
                     RMRKDAEMHADEDRKRKELIEAHNTADNAIYTAEKSLRDLGDKIPAELKSQVEEHVSK
                     VREVINSEDAEKIRQETEALFSVVQQVGTAAYQQEAPSEQAPSEDEQGDEPDSGPDDE
                     EVVDGEFRNV"
     gene            complement(16379..16948)
                     /locus_tag="AC16V7_05265"
     CDS             complement(16379..16948)
                     /locus_tag="AC16V7_05265"
                     /inference="COORDINATES: protein motif:HMM:NF013212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR6256563.1"
                     /translation="MTNESISQSENHEMDQVASGEAQETVEALQATTPDDVQVLKDEL
                     IEWQTKANEYLDGWQRARADYANYKKRVERDQAQAHQNATANAIRRFLEVKDDLELAL
                     KNRPNQGDDAGWADGVELIYRKLAAFLEAEGVSIIETDGKFFDPNFHEAITHEVHPEL
                     ESGQIIAVLKQGYRMGDRVLRPAQVRVAS"
     gene            complement(17020..18054)
                     /gene="hrcA"
                     /locus_tag="AC16V7_05270"
     CDS             complement(17020..18054)
                     /gene="hrcA"
                     /locus_tag="AC16V7_05270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIR6256564.1"
                     /translation="MPKLTERQKLILALVIRSYIETAQPVGSAHLVRRFNLDFSSATV
                     RNEMQTLTELGYLRQPHTSAGRVPTEEGYRYFVRQLMGQSELPVATKHTIAHQFYQAG
                     HDVDRWMRLAASVLAHHSQAASLVTAPHTEQARFKHLELIATHGRQMLMVMVLSGGDV
                     RQQMLSLAEPVSQEQLSAAAAKINALCQGLTPEGLAGVTAQLDVLEKDVIKLVIDDMR
                     RSQDILGGEVYRDGLTHVLSEPEFAEVDVARKALRILEERSLLEDLLARTIMNDAIGG
                     VQVLIGGEGTWEELRDCSMVLARYGVPGLATGTLGVLGPIRMAYGQTISTVRYVAGLL
                     SDLVVDLHAE"
     gene            complement(18178..>18665)
                     /locus_tag="AC16V7_05275"
     CDS             complement(18178..>18665)
                     /locus_tag="AC16V7_05275"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR6256565.1"
                     /translation="KVYHALVTGTPTWQERVVDAPLLPDGDRRHRTIIEGLRGKPALT
                     QLRLLERFQDSTLLEARPKTGRAHQIRAHLAHLDYPILGDRLYGGNDLYLSMLKPDFR
                     GGINGECSILGRVGLHAWSLEFLHPTNGEKLSLIAPYPKDFSGALKQLRKYQPRDIPL
                     Y"
BASE COUNT         3979 a         5225 c         5158 g         4303 t
ORIGIN      
        1 tcgtgaggaa attattctgc gggtaaatca agcacttgaa tatcttggta tctctgagct
       61 gcgcgagcgt gaaacctttc acctttctgg gggagaaaag caaaaagtag cgcttgctgg
      121 ggtgctagcc atgcgcccca gtatcctagt tctggatgag ccattggcga gcctggatcc
      181 tgccagcgcg ttagaaacac tacaattagt acgtcgcatg gccgatgagg gaatgaccat
      241 catgatggtt gagcatagag ttgaagatgt attaaagatc aacccagatc gtgccatgtt
      301 tatgatggat ggtaaggtac actacttagg ctctccagaa ggtttagcca cagtagtcga
      361 ctatcgggaa gttaaactcc cagcccatat gattattgag agagtagcag ccgagtcatt
      421 agctctagag ccgcaagagt tacgcggtgc tgttgatcgt accaccgaaa ccacaccgat
      481 tgttcaattt aaagatgtga catttgaata cgaaagtggt gtggatgtgc ttcatggtat
      541 caatctgact atccgacgtg gcgatgtgat cgctgtgcta gggccaaacg gcgctggcaa
      601 aaccacactg gttaagcatg ctattggctt gctcaaaccc aagtcagggc aggttttggt
      661 aaacgggcgt gacacgcgcg aactcactgt agcacaagtg gccagcaccc ttggttatgt
      721 gttccagagc cccagccaca tgctttttgc tccaacggtg atcgaagagc ttgccttcgg
      781 tccgaccaat ctgcgtcaca gccccgatga aatagaaaag gaggtgagcg aggctattaa
      841 gattgtcaat ctctctggtc gagaggaaga ccctccgttg gcgctctcat ttggccagca
      901 aaaacgtgtc agcatcgctg ccatcttagc catgcgttct cgtatccttg taatggacga
      961 gccaaccgct gggcaggatt ataaaaatta catgagcttt atggattcta ttttgcaact
     1021 gccaaatttc gaggcgatcc tgtttataac tcatgatctc gatttagctg tcatctacgc
     1081 aaatagggtg ctgctggtga gtggaggaca agttgtggat gatggtcctg cccaggaagt
     1141 gttggccgat atcgaatggc tgaaaacttg ccgcctggtg cctactacgc tgcttcaagc
     1201 aaaccttgag cgtttacacc gcaccggacg attcttgcgc gcggaggcat tggctcaagt
     1261 ccaataatgt cttataagag gacttgaaaa ttcagtactt gttagagacc aacgagctag
     1321 accatgaacc cgaatctaat aaacaatgga ggacaacatg aataaactta catctgtagt
     1381 tatagcgctg gtaatcacac tgatactttt cttggcgctg gtgtacgtgg gcggcactat
     1441 attgggtatt gaacggctcc aaacgggcga aaatccagtg ctgcgctttg tattcgtgat
     1501 tatcgggctt ctagttgttt tctttgtcta ctatctcact cgagataaca aggcctggga
     1561 ggtgggaaca cgcgaggtcg tctttatggc tattggggca gccctttacg ccatcttttc
     1621 ctaccttttc aatggtacag tcttcgctgt accctcagtc agtcaggttg cattgcgccc
     1681 agcgatatgt atcccgatct tctttggtta cacctttgga ccattggttg gtttgttcac
     1741 tggtgcggta ggcaatatgc taggtgacgc tttaactggc tttggtcttt acccccaatg
     1801 gagtcttggc aatggtctga tcggcatgat atcggggatg gcttggctgt tcaccgataa
     1861 aaagagaagc ctaaattcag tgctcatcgt cagcgctgta ctagcggtaa tagccacatt
     1921 ggtatattac ttcaaccgta cgcttccaaa catgttgttc ttcgatcccg aacaaaacat
     1981 ttttggtgac cagccgatca cactgtttgc gggcatatct gcagtggtcg gtttcgtttt
     2041 gatattggta gtccggtata tgttcggcca gagtattgat gttacagctg ccgtcacatg
     2101 gggtatgttg ggcaatctga ttggtctggg tttcgcctcg atatctgaca tatggatcaa
     2161 cggttttagc ctgccagtgg ctattgtagg cgaatttcta ccctctgcgg gaccaaacct
     2221 aatcttcacc gctattcttg tgccgttact tgttggtgca tatgctgcga ccaggcggca
     2281 agctggtcga taataatgag cttaggatat ggagggcgat atgtaactcg tttcgccctc
     2341 caaactaatt atttccatta tgctggttac ttggcgctac cgtaagcgta aatcactaat
     2401 acagaccttc gatccgcggg cctggttgat tttttttggc tgctttctgg cgtcaacgct
     2461 cttcttctgg gatttgcgct tcttgctagt aatgcttgca attgctttgt tttttgtttt
     2521 cacctctgat atccattggc acgaatcgcg ccgcgcctgg ctatttattg gtggctttat
     2581 acttttctat gctgcactgg ttttcctgac cgggcgaggt gggactgaac tttatgacca
     2641 ggaatacctg ctcaagcgat tagaagtccc atttacaata tttgggtggc gccctggcat
     2701 taatatcact gcagaacgca taatgttcgc cagcagccag cttgtccgcg tttttagcat
     2761 agctgccatg accatcctga taccatattc gctcaatcca gctcagtatg gtgtggcttt
     2821 tcgtggactg ggtctcccag ataagatagc ctatgctatg gacctgacca tgcggtttat
     2881 ccctactttt gggcgagatt ttcaactgac catggatgcc caacgagcac gtgggtacga
     2941 gattgaaaag ttaagcggag gtttgtttgc gcaggtgcgc aaactggcgc cacttgttgt
     3001 acctgtaact attcacgcca taatgggcag cgaggatatc atagacgcta tggatttgcg
     3061 tgcttttggc gttgggccac gcatgtggct agaacagttg cattaccgga agcgcgaccg
     3121 agtactgata tacatgggta tcctgttatt tatcacttct ttggtggtga cgatcttggg
     3181 ctatggcgag ttctgggttc cggagttctg gtaccagctg gctcaatagc atgccgggga
     3241 ctaaagtcct cggctcagtt caattaagtc cactgaagtg gacataaaaa ccggctttca
     3301 atcaccttca agtggcttcc gagggtctca ggagctgtcc aaaaagaccc aaacgatcta
     3361 tccattaccc ctgtaagcag ataagattgt caataagacc gccaatgttg gcggcttacc
     3421 aagtcgcccc aggtcttttt ggacaatctc gtgggataaa aatcggattt ccgaacgttc
     3481 tcgaatagat taaattacta gccgttaaat acagcctcta ccaacataag caactgcgct
     3541 aacccttgcg agaggggttg ggtgtgaata tattcgccag ttgtatgggc tccgccacca
     3601 gtcgttaagc cgatgcagat tgccggcagg ccgcggctga gcggcaggtt ggcgtcggtt
     3661 gaaccgatat tgaacagcgg ctggatgcct tgcgattcca ggcagctcgc cgccagttgc
     3721 accagcgggt gatctcgtgg catctctccc gcagggcgtt ggccgaccac ctcccaagag
     3781 aactgcacac cgttgcgatt gccggcatcg atcagtcctt ccacttcggt ggctagatta
     3841 accatgcttt cgtgtccctc ggagcgcaag tctaacaggc agtttgcctc cgcggcgatg
     3901 gagttgactg tcgtcccacc cgaaattacc cccacgttca tggtggtgcg tggctggctg
     3961 ggcagcgata gggcacttag acgggtgatc agtcccgcca gctcgtggat agcggagggc
     4021 tgcccataat ccacccaaga gtgcccgcca gctgtactgg cgctaatctg gtagcgccgt
     4081 acattgagac cgcgatgata tacctgcccc aaagccatgc cctccaggat aagataggcg
     4141 cgcgcatcac ccccgaagcg gtctaccacg gcggtcatgc cgagcaggtt gcccatgcct
     4201 tcttcggcca cattggcaac cagccacagg tccccgggta aggcaagcga agatttcgct
     4261 tttagacctg agaggttggg ctgaagcctg ggtgagacaa tttcttttac gccccaaagc
     4321 agaccgaaca accctgccac acccagggcg ttatcaccaa tgccgggacc gtagatcgtc
     4381 tccgcctcgc ggcggcagtc taaaggcgta tcttccggaa agacagtgtc gagatgggcg
     4441 ctgaccacca gcggcggcgt gctccctgcg cccggcaacc tgccatacac gttacctatt
     4501 gcatccattg agatgtcgct cagcccctcc gcttgaaaac gctcaaagat gaaagcggct
     4561 cgccgagcct cggagaatgt gggcgcaggg atctgctgga tggtcatagc cagagccagc
     4621 acgcgctcgc tcagcgcaag acctaacacg gaactcatat tttcctaact ccttgctgta
     4681 aagcctccag cctagcttgt ggcagcgcag gcaggcagtc cagggcataa aaaggaccat
     4741 tcccttcaac taccagcgaa gccgagatgt tgccatgcag ggctgcctgg agaggatcgt
     4801 aagtgcgccg gtagcctgcc aggaagcctc cgcagaaggc gtcgcctgcg ccgctgggat
     4861 ctaccatgcg agaggggtag gccggaatct cccatcgggc gcgcccagct gcgtcgtaga
     4921 gcaattggcc accctcgccg cgcttgatca caataatctc gcaaccatac gaagccaggc
     4981 cctcggccat ttgccacaag tcggtgctgc gcccttggaa tagggagctc atctcttctt
     5041 cggagggtag gaaggccgtc aacccggtca ccagcgctgg tacgtcatcc cagaaagtag
     5101 ggttcatgta acctggcgaa ggatccaggg tgacggtagt gaattccgct tggcgcagaa
     5161 ctgcgggcaa caggctgtgc gtcaggtaat ccagggggca taggtgagcg gcgctggcgt
     5221 ccaggtagtc ggcggggata tcaccttggc gcagtgagat ggatgataga ttcgtgcggc
     5281 tgttaaggtc agacgatgaa tcacggtacc ccagcagttc tttcgggaag gtaagtccag
     5341 cacgggcgaa gtgcgcaacc gggtcatcgg tagcacggtt tgtgcggctg agaaaagcgg
     5401 taaaagcacg cagatccacc ggctgggaga tgacgttcac accgcgtgta tctagccctc
     5461 gccgggtaaa ctgatccagc cactcctggg ggtagtcctc gcccacacgg gccagaacgg
     5521 cgggaggggg ttccggttcc cataatttca atccaacagc agcgtagaga acgttcccac
     5581 cgggtacgtc caggagtgcc tcgcccgaag gcaggatgat atattcgcga agcaacgatc
     5641 cggcgataat gaagcgaggc aattttgatt ccatatgacg aataatccgt taagaattat
     5701 acgttagaaa gaaaagatat aaaagtttgg gtcttgggaa tttggcgtag caggggcaat
     5761 aaagagatgg ttatctggtg gctaaagcca ggaaaacatc ttcgagggtt ggctcggctg
     5821 gatctatgcg taagccttcg atgcccgcct catcaagatg ttggcgcagt gtctcagcac
     5881 tcacatcggc gggcgtgatt gcccgcaggt gatcgctgac caaccccgcc cgcaatacgc
     5941 acgggcatgt ctccagggca tttagggcaa ctagcagtga tgacgccccc gtgctactga
     6001 ccccatccaa gaacacatcc catgtcagcc cgggcagggc gctggttttt agtgtcgatg
     6061 gagcatccat agctagcagc ctgccgtcgc gcatgatgcc gactcgtccg cagtactccg
     6121 cctcatccat atagtgcgtg gtcaccaaag ccgtcacgcc ttcgtccacc agcgcataga
     6181 tcaaatccca aaaagcgcgg cgagtcgtag ggtctactcc gctggtgggc tcgtccagaa
     6241 atagcagctt ggggcggtga acgatggctg tgcccaaagc caggcgctgt cgccagccaa
     6301 ccggcaagtc gcttacccgc tccccttcga gctggctgag ccccaccagc tccagcacct
     6361 ctgccaggcg tgccggcgag cgctcgccgt acaccccggc gtagaaggcc agattttcgc
     6421 ctacagtgag gtcgtggtag agggcgaatt tctgcgacat gtaccccacg cgctcccgta
     6481 tctgctcagc ctggcgaacc acgtcgtagc cgagcacgct ggcctctccc tcggtgggga
     6541 tgagcaggcc cagcaacatg cggatagtgg tggtcttgcc cgaaccatta ggccccagat
     6601 agcccacgac ctcaccggcg ttcatggtga aagagatgtg gtccactgcg gtgaaatcgc
     6661 caaagcggcg agttagattc gttgcatgga tgacttctat actcatactc aatcttgcgc
     6721 ttttatctct gtttttcctc ggcgattccc aatgagacct ttatgtcagg atgatgaatt
     6781 aggttgatcc aacaaagaaa tgaacacatc ctccagctgg ggcgcaactg gacggagcag
     6841 ggtgatttcg ccgccttcgg caaggatgcg ctgtgtcagc cgctcttcga ccaaatccct
     6901 cgagcccagc cgggtccgca ggtgaatgcg gtcgccgaac atctgtacat cttcgacatc
     6961 ctggtcagcg tgtgcaaggt ggcgcagcat atctaagggt tggccacgta gctccaggat
     7021 ttgatccttc aggcgcaggc gcagatcggc aggcgagcct tcgatcagca aacgacccgc
     7081 gcgcatgaag cccacccgcg tgcaacgcgc cgcctcgtcc atatacgggg tgctgactag
     7141 cacggccacc tcggcgtggt cgtggtttgc ctggcttgcc aggcggatga tcaactgcca
     7201 gaaatcctgg cgggtgactg ggtctacccc ggtagtgggc tcatctagta gcagcacgcg
     7261 cggcctgtgc accagcgctg ctgccagtcc aagtttctgt ttcatgcccc ctgaaagatg
     7321 acctgccagc cggtcgacaa acggatccag cccgacgaag cctaggattt ccatacagcg
     7381 cggtcgccaa tccgccgagc tcatgccgcg cacctcggcg aagaagcgga tgttctccag
     7441 cacggtcagg tcttcatata gcgaaaagcg ctggggcaag tagccgatct gagcgcggac
     7501 gagctcagtc tggcggactg catcataccc actgatccgg atggaggatt gctcgtcgtc
     7561 tttgattcgg tcaagttgca gcgccgcgca cagcaggcgt agcgtggtgg ttttgccagc
     7621 gccgtccggt ccaaccagac catagatctc ccctgccgcc acgcgcaggt ttaacccatc
     7681 cacagcccgc gtttcgccaa acgacttatg caaattaagg gcttcaataa gtatgtccat
     7741 agagatgggg gtgaaagtgg tgaaattgcc gaacactttc acttgagact aaagtctaca
     7801 tccgcgggca tgcccggctt gagcttgccc tccggatttg tcaccgagag ttccactgca
     7861 aacacggttg tacggcgacc ggcttcggtt tgcacgttgc gcggggtgaa ctcggcctgg
     7921 tcagcgatgc gtatgaccgt ggcctcaaag gcctcgcttg gatacgagtc cactgtcacc
     7981 gaagcggtct gtcccagctt gatggtgcca tagcggtctt cgggtacata tacggttata
     8041 gtaagatcat ccaatctgag tagagatagc aggggtgcgc ccggaaccag cacctcgccc
     8101 ggttccgccg tgcgggtcag cactacccca tccattggag cgttgatgac cagtttggcg
     8161 atctgcgctt caatcacttc catcgcaccc tgcgcgactt ccacctgtgc ctgggcagcc
     8221 gccagttgct cggggctggg gtcggctttg gcaaccgcca ggttggcttc ggcagtgttc
     8281 agatgcgcct gggccagggc cagggagtca ggatcgatgc cttcctgtaa ttcttcgtat
     8341 tgccgttttg catcggccag cttagcttct gcaaggctta cctccgaacg agccaaggct
     8401 agttcatact ggttggcggt gccgaccaga ttgttgtaac gctcgaccat ggaatcgtac
     8461 tgcttctgtg cagaggccac ctgtgcctga aagatggcgc gtgtcaggtt gctctgatct
     8521 tttttctcat acggctcgaa gtcctctaag gcgtcctcca gcttatcctg ggcaataacc
     8581 accgaagccc atgcggcgtc gatgtctgag gggtcagcct cggtattgac attgtccaag
     8641 tattgctgcg ccttatcgcg ggatttatca atggcagcga tggactgctc gagctgtgat
     8701 tccgtcaagc ctgaattatc gtacagggct tgcagtgcct gccgggcagt cgtcagctcc
     8761 atctccgctg aggcgatggc aagtcggcgc tgctcatctg tcggtccttt ggcggctaaa
     8821 tcgtagttgg cctgggtcaa atgcaatgca gcccgtgctt gacttaactg cgcctgcagc
     8881 agggagtcgt cgaagcgcac tagggtctgc ccggcttcca ccacctcacc ctcctggacc
     8941 ataacttccc gtacacgccc gccaagttca ggggcgatga ccacctgtgt gccctcgatg
     9001 gtccctgagg cggttaacgc actggaattg ccgttcccct gcccgagggt aaaataccag
     9061 atcgcacccc caaggatggc gatcagcaaa acaataggca ctatctttct aggatttgga
     9121 tgcatgggaa cctcctacga tgatcagtcc agtcttttcc ggaacctcat cgccgcggct
     9181 gccataatgg caaaaccgaa aattgcaagc gccaggatat cgctcttgag catgtccgcc
     9241 cccacaccct tgagcagcag cgagcggatg atcaccagat aatagcgcag cggcatcagg
     9301 taagaaagcc actggagcac tttgggcatg gcctccagcg ggaagaagaa cccggagagg
     9361 aagatgctgg gcagtagcgt catccatacg gtgagcatgg cctcctgctg cgtgttagcg
     9421 atggtagagg ctaagaggcc gatccccaga ctggtgagta ggaataacgc ggagagcagc
     9481 aaaatcagac ccaggtcgcc gcggataggc acatgaaacc acaaagcgcc aatggcgagc
     9541 acttccagcg tattaagaaa ggccaaaatg acgtagggca acatcttgcc caccaccagc
     9601 tcccaggggc gaataggggt gacgatgagc tgctcgatgg tgccgcgctc gcgttcgcgc
     9661 acgatggctg tggaagtcag gatagaggtg atggcgtaca ggatcatccc aatcactccg
     9721 gggatcataa agtaagcact ctccaagtct gggttgtacc aaacctgggt gcgcacttcg
     9781 atgggcgggc ggatggcgga ggactgcccg cgccgaccca ggcggtcgct gagcacactg
     9841 gtagcatgct gctggccgat cacctgcgct gccgagaggg ccgtggctgc caccgtgggg
     9901 tcgctgccgt ccaagataaa tgccacctct gcttggccat ctccctgtaa gcgggagctg
     9961 taatctggtg ggatgaccag gccgccgcgc gcctggcctc tgtcgatcaa gtcgcgcagt
    10021 tcctgctcgg agctcacatc gtactccaga gaaaagtaat tggcggcgcg gtaagcgtcc
    10081 agcaaggcgc gcgcttcagg cccacgatcc tggttgtaga ccgccagcgg cacgttgcgt
    10141 acatcgttgg tagctgaata gccgagcagg aaaagttgca ctaatggcat gaccagcgcc
    10201 agcgctaggg tgcgcgggtc gcgcaagatc tggagaaact ctttgcggat cagggaggtc
    10261 agacgagagt tcattgttgc accttctcga gtgtagtgcc acccttcaag attccgtata
    10321 ggtaaatatc gacgaaataa tccaaggcat gctcgcgctc ctctggcggc aactggtctg
    10381 cgatcatgat ctctgtgatc aggagagaga agaagagtcc gatgaaggcg cgcaccacca
    10441 ccaaaagcgg gatatcgcgc agctcctggc gctcggtctg gaaacgctgg gtaaactcca
    10501 tcatttcggg gaagatgatt tgaaagatgt gtgggacatg ctgcccctta aattccacca
    10561 gctcgatgaa catcaggttg aggaaatcgg tgcgttgccc taaaccggcc accatgcgct
    10621 gggcagcatc gcgcacaaat tcttctaccg tctcgccctg cgcggtgttc atcagaggca
    10681 gtacatcccg aaagggatgg cgttctagga agactgctgt gaatatgtct tccttgctgg
    10741 agaaatggtt gtatatgcca cccagagcga taccggctgc ttgagcgatc tggcgcatag
    10801 acgtgccatg gaaaccgcgc tccaagaaca attggtaggc ggcctgaagg atgtcgttgc
    10861 gggtgcgctc cccgcgaggc gcgatctctt cagacatagg agtttctcgc tctatactga
    10921 acgaacgttc gttcatatta tagatagaga tagttcgcct gtcaagagct tgcaatgagc
    10981 gtagctatca aatgagggtt cgagggttct ttgaaaaagt tattgcgagg tacccctaca
    11041 cacagggcgc acatccaccc caagagtcac agcccgttat cgcgaccctc gcaaggtaag
    11101 cttatatggc aagattttgc cgtttatgta ggttactact ttaacgcatc ggtcttggct
    11161 gccatgatga aatcgttgcg atgcaggcca ttgatcttgt gagtccacca tgtcacggtc
    11221 accttgcccc actcggtgag cagcgccggg tggtgatctt cttcttcagc tagcgcgcca
    11281 actttgttcg tgaattccaa ggcttgggca aaattcttga atttaaatac ccgctgcaac
    11341 ctttggatac cctccacatc gagcacctgc cattctggga cacccggatg caagccggca
    11401 atctgttcac tggttagagg gggcactccg ccgcggcaag gcacacattt catttctttt
    11461 aattcttcca tctgataatc tcctgcaaaa tcctaccaat aaaaaggggg cggtcagtcc
    11521 gccccgatct gtaatctttg gatatttatc ctgggttatt tctcgccctc acccagctgg
    11581 gccacttgac gcagcaagcc gaacaccagc actcctagtt tcaccccatc acctggggag
    11641 atgcgcacct ccccatcttc gcgctcagct cgctgaatga tgaggtaggc agcgctaaga
    11701 cctaccactg cgcctaaaac tgccccagct agcagagcct tggatttcca gtttccgcca
    11761 gcctgttcat ttccattgtt tgacattaat cactcctttg ctcacttgcg tcgcagcgcc
    11821 cgcagccctg ctgtgaagct ttgaacacgt aaggtcggct ccacgatagc atcagcggct
    11881 ttcctcaccc gcgcatcaat caaaacaaat acatcctgga tttggcgggt atatttaggc
    11941 agggcgccaa ttagccatat gattccatac gccagacccg ctgtcactac cagaaagatg
    12001 agggttaaaa acatggtcgg gatgatcagc catatgatgg agatgctcgc ccaacgatcc
    12061 accgggcctg tctgggtcgc tccagcgtaa accacaccag cccccgcagc cagcaccagc
    12121 agcgcgccga gcgctaatgg cagcgtaatt tgccagagaa cctcctggcg atgcttgcgc
    12181 tgggttaccg gattgggttt gggggcagaa ggtgggaagg tgttctcatt catgtattta
    12241 ttataatatc atccccgcca ctagcgctac ccaatccccg gactggcggc gatccagggt
    12301 gcgcaggttg tatctttgca gggcgtcttc gacctcaacc gcttgctctt ccaggatgcc
    12361 tgaaaggatc aggacgccgt cgggtgtgag cagatcgggc aggccatcat ccagcaagcg
    12421 aatgatcaca ggtgccagga tattggccag caccaggttt gcgcggcgaa gcgcaaattc
    12481 accccggcgg atttcgtcaa cagaaccctt gccaatttcc agctgacccg ccacgccgtt
    12541 cagttcggcg ttatcgcgcg tcgcctgcac ggcttccaag tcgatatcca cgcccagggc
    12601 ttgttccgcg ccaagtttga gcgccgccac ggcaaggatg cccgatccgc agcccaggtc
    12661 gataattgcg gcttcccttc cgggaagata aatttccatc agctccaggc agagctgtgt
    12721 agtggggtgt gtgccagtgc caaaagccat gccagggtcc atgcgaatgg cgatgcgtgc
    12781 tggatcggga ggctgctccc aggctggcac gaccatcaac cgctgaccga tggcgatggg
    12841 gtgataatga gacttccagg cttgtgacca gtcttgttcg tgtatgatgt ggtattgtgg
    12901 ggctggcaaa ggacggatac gccccaggaa ccataaagcc tgctcgacgc gctggcggtt
    12961 ctcgtcaagc tggtcgtcaa caggcaggta accgcacacg cgcagcggac cgatagcctg
    13021 cccttcgctg tcgtctgccc tggctgtcac tgccgtagac tcgatggcga caccgccgga
    13081 gacatagcgc cctagcacct ccgctacggc ctcagccatc tctccatcga caatcaaaga
    13141 tatctcaagc cagcttgaat tacccattca gcctgtattc cgatctttcc cccgctccaa
    13201 agggggttag aggtgagtga agtgtaagtc ggggcgactc gagggcatta actaccctcc
    13261 caaggtttct ttcaaccagt caataaagct gcgctcttgc gggcgcactt cgctccccag
    13321 gctttttgcc agttgctcaa aaagctcgcg ctgttcactg gtcaagcggg tcggaatatc
    13381 tacattaacc accactaact ggtctccacg accgttgctg cgcaggtagg gcactccttt
    13441 gctacgcata cgcagtacct tgccaggctg cgtaccggag gggatcttca atcgcgctgg
    13501 accatctaca gtaggtattt ccacctctgc tcccaaggtc gcctgcgcca ggttaatgtc
    13561 caggtcaagg atgatatcgt tctccctgcg gcggaagaat ttgtgaggct ggacatggat
    13621 ggtgatgtaa aggttgccgt tcggcccgcc gttcaagccc ggctgccctt ccccagccag
    13681 gcggatttgg gtaccgtcat ccactccgcc cgggatgttc acgaccttgc gacgggttcg
    13741 ccgctccagg ccgcgaccct ggcaagtatg gcagggtgtg gtgatggttt cgccgacacc
    13801 tttgcaggtg ggacaggtag atacctgaac catggacccg agcagggtct ggcgaacctg
    13861 gcgcatctcg cctgagccgt tgcaggtcgt acaacgaacc ggggaagtgc ctggctccgc
    13921 gccagagcca gaacaggtgt tgcacaactc atcgcggaca aactcgatct ctttctccag
    13981 gccaaatacg gcttcctcaa aggtcagatc aagccggtac gagaggtctt gtccgcggcg
    14041 gggtgcgttg cgctggcggc gcgagctgcg tgtgaagcca ccaaagccaa ataaatctcc
    14101 gaagatatcg gcgaaatccg agaaatccac gctgaaatca ggtactccgc cggctccctt
    14161 cactccggcg tgacctaagc gatcgtaaac cgagcgttta tcctggtcgg atagcacggc
    14221 atacgcctcg ttgatctctt taaagcgttc ctcggcgtcg ctttccttgt tgacgtcagg
    14281 atggaattgc cgagccaaac gccggaaagc ggacttcagc tcttcattgg aagcgttctt
    14341 cgggacgccc aggatttcgt aataatctcg ttcagtcatc atggattatg gttgccggat
    14401 gcaggcagat acgagcagcc tgctagcctc caacctgttg tcattgaacc aataagcatt
    14461 aaacgtttct aaactcacca tcgacaactt cttcgtcatc aggtcccgaa tctggctcgt
    14521 cgccttgttc atcttcgctg ggcgcctgct cgctgggcgc ttcctgctgg taggcggcgg
    14581 tgccgacctg ctgcaccacg ctgaacaacg cctcggtttc ttggcgaatt ttctccgcgt
    14641 cttcgctgtt aatcacctcg cgcactttgg aaacatgctc ttccacctgg ctcttgagtt
    14701 ctgcggggat tttatcgccc aggtcgcgga gcgacttttc ggcagtgtag atggcgttgt
    14761 cggccgtgtt gtgcgcctca atcaactctt tccgcttgcg atcctcatca gcgtgcatct
    14821 cggcgtcctt acgcatgcgc tcaacttccg cttcggcgag ccccgatgag gcggtaatcg
    14881 tgatgttctg gctgcgactg gtagccttgt cctgtgcggt gactttgaga atgccgttgg
    14941 cgtcgatgtc gaaggtcacc tcaatctgcg ggatgccgcg cggcgcgggt ggaataccat
    15001 ccaggatgaa cttacccagc gatttgttat cgtttgccat ggggcgttcg ccctgcagta
    15061 catgaatctc cacctgaggt tggctgtcgg cagctgtgga aaaaatctgg cttttccgcg
    15121 tggggatggt ggtgtttcgc tcaatcatcg ggctggctac gctgcccaag gtctctacag
    15181 atagggtcag gggcgtgacg tccagcagca agatatcttt cacctcgccg cccagcacgc
    15241 ctgcctggat cgctgcacct accgcaacca cttcgtcggg gttgacaccc ttatgggggc
    15301 ctttaccgaa cagtttgcga accgcgtcct gcacagcagg catacgcgtc atgccgccca
    15361 ccaatacgac ctcgttcacc tggtctggtt ctagcccggc gtctttcatc gcctgtcgca
    15421 ctggcccaag cgagcgttcg atcaagtcgg cagtcaactg ttctagcttg gcgcgagtca
    15481 gcgtcatgac cagatgcttc ggtccactgg catctgcggt gatgtatggg aggttgatct
    15541 cggtctgcat catgctggaa agctcgattt tagctttctc agccccctct ttcaagcgtt
    15601 gcagggcttg cctatctttg cgcaggtcaa tgccttgttc gcgcttaaac tcgtcggcta
    15661 taaaatcaat gatgcgctgg tcgaaatcgt cgccgcccag gaaggtgtcg ccgctggtcg
    15721 cgcgcacctg gaagaccccg tctcccacgt ccaagatgga gatatcgaag gtgccgccac
    15781 ccaggtcata gacggcgatg agctcgtcct ttttcttgtc caggccatag gccagggagg
    15841 aggcggtcgg ctcgttgatg atacgcagca cctccagccc ggcaatcttg cctgcgtcct
    15901 tggtggcatt gcgctgggcg tcgttgaagt aagccggcac ggtgatcact gcctgggtga
    15961 ccgtctcgcc caggtaggcc tcagcatctg ccttaagttt gcccaggatc atggcgctca
    16021 cttcgggcgg agaatattcg cgctcgtcaa ggatcacgcg cacatcgcca ttgggcgcct
    16081 gtgctacctt atatggcacg cgtgtcgtgg cgcgctgcac ctcgggatcg ctaaacttgc
    16141 gtcccatgaa gcgcttgatc gagaagatcg tgttctcggg gtttacgaca gcctggttgc
    16201 gcgccacgcg cccgatcagg cgctcccggt tcttgttaaa ggcgacgaca gacggcacca
    16261 ggcgctcgcc ctccgccgag gggattacca ccggctcccc gccttccatg acagccacga
    16321 ctgagttggt agtacctaaa tcgattccaa taatttttcc cataatgaat tcctccgatt
    16381 agctagctac ccgaacctgg gcggggcgca acacccggtc acccatacga tagccttgtt
    16441 taagcacagc gataatttga ccactctcca gctcgggatg gacctcgtga gtgatggctt
    16501 cgtggaagtt tggatcgaaa aatttgccat ctgtctcgat gatgctcaca ccctcggctt
    16561 cgaggaaggc agcaagcttg cgatagatca actccacccc gtctgcccaa ccggcatcat
    16621 cgccctggtt tgggcgattt ttgagtgcca gctccagatc gtctttcact tccaggaaac
    16681 ggcggatggc atttgccgtt gcgttttggt gtgcctgggc ttggtcacgt tccacgcgtt
    16741 tcttgtagtt ggcgtaatcc gcccgggcgc gctgccatcc atccaggtat tcattggctt
    16801 tggtttgcca ttcgatgagt tcgtccttta atacctgaac gtcgtctggt gtggtcgctt
    16861 gaagcgcctc gacggtttct tgtgcttctc cggaggctac ctgatccatt tcgtggttct
    16921 ctgattggct gatactttcg tttgtcattg ctccttgtcc tgcaaacagg taaatttctg
    16981 ataacgatca aaaactcatc aaaaaactgt gatgctcgat tattcggcgt gtaaatcgac
    17041 aaccagatcg ctcaaaagcc cggccacata gcgcacggtg gagattgtct gaccgtaagc
    17101 catgcggatc ggccccagca cgcccagggt gcccgtggct agacctggca cgccataccg
    17161 agctagcacc attgagcagt cacgtagttc ctcccaggtg ccctcgccgc caatgagaac
    17221 ctgcacaccg ccaatggcgt cattcatgat cgtgcgggca agcagatctt cgaggagcga
    17281 acgctcttct aaaatgcgca gcgctttgcg ggccacatcc acctcagcga actcgggttc
    17341 agacagcaca tgcgtcagcc cgtctcgata tacttccccg cccaggatat cctgcgagcg
    17401 gcgcatgtca tcgatgacta gtttgatcac gtccttttca aggacatcca gctgggcagt
    17461 cacgccagct aaaccctcgg gcgtgagccc ctggcacagt gcgttaattt tagcggctgc
    17521 agcgctcaac tgctcctggg acactggctc tgccaggctc agcatctgct ggcgcacatc
    17581 gccgccagag agaaccatca ccatcagcat ctgtcgcccg tgcgtagcga tcagctccag
    17641 atgcttgaag cgtgcctgtt ccgtgtgtgg ggcggtcacc agcgaggcag cttgcgaatg
    17701 gtgcgccagg accgaagcag ccaggcgcat ccagcgatcc acgtcgtgcc cggcctggta
    17761 aaactggtga gcgatagtgt gcttggtcgc tacgggcagc tcagactggc ccatcagctg
    17821 gcgcacgaag tagcgatatc cctcctcggt gggcacgcgc ccagccgaag tatgtggctg
    17881 tcgcaggtaa cccagctctg tcagggtctg catctcgttg cgcaccgtag ctgagctgaa
    17941 atccagattg aaacgtcgca ccaggtgggc cgatccgacc ggctgcgcag tctcgatata
    18001 gctgcggatc accaaggcca ggatcagttt ttggcgttcg gttaatttcg gcattttcac
    18061 ctcattgaat ttagcactct cgaggcgaga gtgctaaaaa agcgcaataa atgataccat
    18121 gcccgcatga gaacgtcaag aaacgaggtg atgcgagctg acaattctga tatttggtta
    18181 ataaaggggt atatcccgtg gttgatattt gcgcagctgc ttcagcgcgc cagaaaaatc
    18241 cttcggatag ggagcgatga ggctcagctt ctccccattc gtcgggtgta aaaactccag
    18301 cgaccaagcg tgcaagccca cccgccccag gatgctacat tcgccattta tgccgccacg
    18361 aaagtctggc ttgagcatgg acagatacag gtcgttacca ccgtacagcc tatcgcccaa
    18421 gattggataa tccaggtgcg caaggtgggc gcggatttgg tgtgcgcggc ccgtcttggg
    18481 tctggcttcc agcagagtgg aatcctgaaa tctctccagc aggcgcaact gcgtcagtgc
    18541 tggcttgccg cgcaaacctt cgatgattgt acggtgcctg cgatctccgt ccggcagcaa
    18601 tggcgcatct accacgcgtt cttgccaggt gggtgttcca gttaccaggg catggtagac
    18661 cttgg
//
LOCUS       DBNHOH010000294         2277 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1747932, whole genome shotgun sequence.
ACCESSION   DBNHOH010000294 DBNHOH010000000
VERSION     DBNHOH010000294.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1747932"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..1042)
                     /locus_tag="AC16V7_05280"
     CDS             complement(14..1042)
                     /locus_tag="AC16V7_05280"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR6256566.1"
                     /translation="MPKNYLVTGGAGFIGSNYTARLLQRGENVTIYDNLSRAGGPLNL
                     RWLEDTFGKGSFRLVEGDLRDAALLTANTRDADVIVHLAAQVAVTTSVVDPRDDFEIN
                     ALGTFNMLEAARLSERKPAILYASTNKVYGGMEDVRVDENESRYHYADYPYGVPETFP
                     LDFHSPYGCSKGAGDQYVRDYHRIYGLPTVVFRQSCIFGPRQFGVEDQGWVAWFIIAT
                     LTGKPITIYGDGKQVRDVLYIDDLLDVYDAALAQIDAVTGEVFNIGGGPNNVMSVWGE
                     FGPILKRLLGSKIQVSWGDWRPGDQKVYISDIRYAEKKLGWKPKINVEAGVECLFEWA
                     RENRKLFT"
     gene            complement(1130..2125)
                     /locus_tag="AC16V7_05285"
     CDS             complement(1130..2125)
                     /locus_tag="AC16V7_05285"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HIR6256567.1"
                     /translation="MSFGPLYIAGPERSGTSLIYALLASHPNIAMTRRANMWTHFFNQ
                     YGDISRPKNFERCLSMMMRYKRLLLLKPDPERIRREFWQGEQTYARLFALLEEHYAEQ
                     VGKPRWGDKSLNTERYADAIFAAFPEARILHMVRDPRDRYSSALTRWKVSRGGVGAGS
                     AIWLSSIKLAQQNQEKYPDQYKIIRYETLVNQPEETMREICAFIGEEYTPAIFSMDGA
                     KTFRDEGGNSSYGQHEPGRISNKSIGRFRQVLSKRQIAFMQSMAGREMEAFGYILDNI
                     HLSPFEQSLYYLLDVPINLARMAAWLLREAYLDQIGRPVPSYRIVAGPDAIKAGA"
BASE COUNT          575 a          574 c          515 g          613 t
ORIGIN      
        1 gtccttagtc cacttacgta aatagtttcc ggttctccct agcccactcg aataaacact
       61 ccacgccagc ttcaacattt atcttgggtt tccatcccag ttttttctcc gcataccgga
      121 tgtctgaaat gtaaactttc tgatcgccgg gtctccaatc accccaggat acttggatct
      181 tgcttcccag caaccttttc agtataggtc caaattcgcc ccataccgac atgacgttat
      241 tagggcctcc gccaatgttg aaaacttcac ctgttacagc atcaatctgt gccagagccg
      301 catcgtagac atcgagaaga tcatcgatat acagcacatc acgtacttgt ttgccatcgc
      361 cataaattgt gatgggttta cctgttaacg tggcaataat aaaccaggct acccagccct
      421 ggtcttcgac tccaaactgg cgagggccga aaatgcaact ttggcgaaag acgacggtgg
      481 gcaagccgta aatccggtga taatcgcgca catactgatc tcccgctccc tttgaacagc
      541 cataaggtga atgaaagtcc agcgggaatg tttctggaac accgtagggg tagtcggcat
      601 aatgatagcg cgactcgttt tcatcaaccc gcacatcttc catgccgcca tataccttat
      661 tggtagaggc atacaagatc gctggttttc gctctgacaa gcgggctgct tctagcatat
      721 tgaatgtgcc cagggcattg atctcgaagt catcgcgcgg gtcgaccaca gatgtggtaa
      781 cagccacttg ggcagccagg tggacaatca catcagcgtc gcgagtattc gcggtgagca
      841 aagccgcatc gcgcaggtct ccttccacca accggaaaga accttttcca aaggtatctt
      901 ccagccaacg cagattgagc ggtccaccag cccgagaaag attatcatag atggttacat
      961 tctcgcctcg ttgtagcagg cgggcagtat agttcgagcc aataaaacca gctcctccgg
     1021 taactaagta gtttttcggc atattaattc ctgtttataa atgatattat gtctattcac
     1081 tggagaaatt cagctttttg tggtgtatca aatgaattta gattgattat tacgctccag
     1141 ccttgatggc atctggtcca gcgacaattc gataagatgg cactggacgg ccaatctgat
     1201 ctaagtacgc ttctcttaac agccatgcag ccatgcgcgc cagattaatt gggacatcta
     1261 ataagtaata caagctttgc tcaaaaggtg aaagatggat attatccaat atatagccaa
     1321 acgcttccat ctcgcgtccg gccatgctct gcatgaatgc gatttggcgt ttagataata
     1381 cctgccgaaa acgcccgatt gatttgtttg aaatgcggcc tggctcgtgt tgtccatagg
     1441 agctattgcc gccctcatct cgaaaagttt ttgcaccatc catgctgaag atggctgggg
     1501 tgtattcttc accgataaat gcgcaaattt cgcgcatcgt ctcctcaggt tgattgacta
     1561 aggtttcgta acgtatgatc ttatactgat caggatattt ttcttggttc tgctgggcaa
     1621 gttttatgga tgagagccat attgcgcttc ctgcgcccac acctccccgg cttactttcc
     1681 agcgggttaa tgctgatgaa tatctgtcgc gtggatcacg caccatgtgg agaatcctgg
     1741 cttcgggaaa agctgcaaat atggcatctg catagcgctc tgtattcaat gatttatccc
     1801 cccagcgcgg tttgcctacc tgttccgcat aatgctcctc aaggagggca aataagcggg
     1861 cgtaagtctg ttcaccttgc caaaactcgc gacggatacg ttctgggtct ggtttgagta
     1921 gcaacaagcg cttgtaacgc atcatcatgg agagacaacg ctcaaaattc tttggtcggc
     1981 tgatgtctcc atattgattg aaaaagtgag tccacatgtt ggcccgtctg gtcatcgcga
     2041 tattagggtg ggatgctaat aacgcgtaga tcagacttgt tccacttctc tctgggccgg
     2101 caatgtatag tggtccaaaa ctcatgtatc ttcctccagt ttctttctag acgacaaaca
     2161 aaataagtgc aataaaaggc agctcaatcc tgagcgtaag gtcaatggct tttacccatc
     2221 cggcttcgcc aagctgccat accagccagg ttagctggcc aatcgatgaa taagtat
//
LOCUS       DBNHOH010000295         1488 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1106723, whole genome shotgun sequence.
ACCESSION   DBNHOH010000295 DBNHOH010000000
VERSION     DBNHOH010000295.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1106723"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1316
                     /locus_tag="AC16V7_05290"
     CDS             <1..1316
                     /locus_tag="AC16V7_05290"
                     /inference="COORDINATES: protein motif:HMM:NF042614.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6798 domain-containing protein"
                     /protein_id="HIR6256568.1"
                     /translation="TVILAIHSIQGQIFARKALDFDLEILHFGLAGQYLLGLDFQNSS
                     FGVFLLLSMHAFLQRKTVWAILWLSLASIFHSAYLFSAALMTLAYLGLLFYENFRKGN
                     TTGYSPASLPQGSQEERSNTGVSEFTRHTLIQRVVMAAKAPFLHGLLALVLVSPVVIY
                     QQTALSSTSPQLSAQALDILVNQRIPHHTLPKVWMDGGAYLQIAIMLAGLLLVRKTRL
                     FPVMLALTLGGALGWFIQVATGSNSLAMLGPWRVSVILVPLSTCLLIAWPIAKFFDRF
                     ARQVEVILPVVVVLCLAGIVYLGREGLAIQRGRFARYERMDSHPMMYFIRQEKAPGQV
                     YLIPPRDNRFDEFRTFTGAPAFINWKSHPYRDFEVLEWYKRNMLARDFYTDDQATACQ
                     TLDKLTAEYMLTHVVIDLQQPHAICNGMQEAYRDQYYAVYSLVKQ"
BASE COUNT          292 a          451 c          383 g          362 t
ORIGIN      
        1 ttacggtgat cctggcaatt cactccattc aggggcagat tttcgccaga aaggcgttgg
       61 acttcgatct ggaaatcctg cattttggcc tggcaggtca gtacctgctc gggctagatt
      121 ttcaaaacag ttcgtttggc gtcttcctgc tgctctctat gcatgccttc ttgcagcgaa
      181 aaactgtatg ggctatccta tggctctccc tggccagtat attccattca gcctacctgt
      241 tcagcgccgc cctgatgacg ctagcttatt tgggcctcct gttttacgaa aatttccgta
      301 aaggaaatac tacggggtat tctcctgcct cccttcccca aggaagtcag gaagagaggt
      361 caaatactgg ggtgtctgaa ttcacccgtc acacccttat tcagcgagtg gtgatggcag
      421 ccaaagcccc ctttctgcac gggttacttg ccctggtcct ggtctccccg gtcgtgatct
      481 atcaacagac agctttgagc tccacctcac cgcagctctc ggcacaggcg ttggacatcc
      541 tggtcaacca gcgcatccca caccatacct tgcccaaggt gtggatggat ggcggcgctt
      601 atcttcaaat tgccatcatg ctggctgggc tgctgctggt gcgtaaaaca cggttattcc
      661 cggtcatgct tgcgctaacc ctgggaggcg cgctgggctg gttcatccag gtggcaactg
      721 gcagcaacag cctggctatg ctgggtccct ggcgagtctc ggtcatcctg gtgccgctct
      781 caacctgcct gctgattgcc tggcctattg caaaattctt cgatcgcttc gcccgccagg
      841 tggaggtcat ccttccagtg gtggtggttt tgtgcctggc agggatcgtc tacctgggac
      901 gcgaagggct ggcgatccag cgcggccgct ttgcccgtta tgaacggatg gacagtcacc
      961 caatgatgta ttttatccgg caggaaaaag cccctgggca agtctatctg attccccctc
     1021 gcgacaaccg cttcgacgag tttcgcactt ttaccggcgc cccggccttc atcaactgga
     1081 aatcacaccc ctaccgcgac ttcgaggtgc tggagtggta caagcgcaat atgctggcgc
     1141 gcgacttcta caccgacgac caggcaaccg cctgccagac cctggataag ctgactgcag
     1201 aatacatgct gacccatgtc gtcatcgacc tgcagcagcc ccacgctatc tgcaacggca
     1261 tgcaggaggc ttaccgcgac cagtattacg ccgtgtattc tctcgtaaaa cagtaagtcg
     1321 ggattacttt cccagaaaat atcctatgcc cgcaaccgtc tcatcttgcc ccttgtgtgg
     1381 gagcgaacac agcgccccct ttgacagacg cacttttcgc aggcagcccg tggtcaaccg
     1441 cttgtgccag tcttgcggcc tggtctacca atcgccgcgc atggatga
//
LOCUS       DBNHOH010000296         4454 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_273403, whole genome shotgun sequence.
ACCESSION   DBNHOH010000296 DBNHOH010000000
VERSION     DBNHOH010000296.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_273403"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="AC16V7_05295"
     CDS             complement(<1..629)
                     /locus_tag="AC16V7_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR6256569.1"
                     /translation="MAIKTYGTMQVDWEQRIDFERLRRERLDRAKKLLKESEMGALLC
                     FDMNNVRYITATHIGTWAQDKVNRFTLLPQDDEPILWDFGSAAKHHQLYAPWLGEKRS
                     RAGISLLRGAMSPEMGRAEDVANKIWAELKERGLEKEPVGVDIAEVPVLFELQKKGLT
                     IVDGQQLMSEARVIKTQDEIALLTHAAAQVDAAYDELYRAMKPGMRENEA"
     gene            complement(649..1635)
                     /locus_tag="AC16V7_05300"
     CDS             complement(649..1635)
                     /locus_tag="AC16V7_05300"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR6256570.1"
                     /translation="MAEREITFAEAIREALAEEMRRDQNVFIIGEDVAEAGTPFKVLS
                     GLVEEFGKNRVFDSPISEPGFTGMGVGAAMTGMRPVVDIMFGDFLTLAMDQIVNQAAK
                     VHYMSGGKLKVPIVFRTTMGATRRSAAQHSQSLQAWVSHVPGLKVTMPSTPYDAKGLL
                     KTAIRDENPVVFIEDKMMYKIKGRVPAEEYTIPFGVADIKLQGDDITLVATSSMVQVA
                     LGAAEKLKDIGISAEIIDPRTTTPLDKQTLIESAKKTSRAIVIDEGYEQYGVTAEIAS
                     VIADGAFYYLDAPVKRMGAMNVPIPFSPVLEDLTVPTEETVFEMAKSLCGKA"
     gene            complement(1672..2676)
                     /locus_tag="AC16V7_05305"
     CDS             complement(1672..2676)
                     /locus_tag="AC16V7_05305"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR6256571.1"
                     /translation="MANETQVTTNPKPETLDVEQWLHAYEQMAKIREFEEKANELYMK
                     ALMPGLTHLYSGQEAVAVGVCEALRRDDYITSTHRGHGHCIAKGASVDLMFAELLGKE
                     AGYCRGKGGSMHIADPESGNLGANAIVGGSAGIATGAALSAKMRGTDQVAVCFFGEGA
                     LGQGLLYEVMNMASLWKLPIIYVCENNLYNEYTHYSEALAGEVTERARAFGIHTESIY
                     GQDVQLVYETMRKLVERARRGEGPAFLECLTYRYYGHHVGDINRTYYRSKEEEQEWRE
                     VHDPLKLLEEKLVASKLTDQDVLERIYADAKNEVEAGVQFAIDAPYPDQSQVTEDVYA
                     "
     gene            complement(2676..3554)
                     /locus_tag="AC16V7_05310"
     CDS             complement(2676..3554)
                     /locus_tag="AC16V7_05310"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6256572.1"
                     /translation="MANLGFIGLGVMGGRMVKRLLDAGHSVTGYNRTRSKAQWLLEAG
                     MTWGNTPRAVAKSADIILSMVTNTEALHSIAEGPDGLLAGLSAGKIYVDMSTVSPAFS
                     RELAKEVAIQGASMLDAPVSGSVVTLEEGRLSVMVGGDAETFEKAKPILQDIGPKVTH
                     VGANGLAVTMKIATNLSLAVQMLAFSEGVLLAEKSGIKREIAVEVLVNSVIASPMVKY
                     RGPFVLEMPDEAWFDCYMMQKDMNLALELGRELSIPLPTTAVTNEFLSAARGMGLEKQ
                     DFAIMFDVLAQMSGVK"
     gene            complement(3576..4274)
                     /locus_tag="AC16V7_05315"
     CDS             complement(3576..4274)
                     /locus_tag="AC16V7_05315"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR6256573.1"
                     /translation="MYQPIQSERLYEQIVDQIERRILAGDLKIGDQLPAERELAEQFA
                     VSRTAVREAIKALREKGLVEIRLGRGTFVTSGAAGAVRRSLGLLLKTENGFVNLEEVR
                     EILEPEIAALAATRISEEYITAMTEAVETMDTALDNVEIFVEADLDFHLALAEATQNP
                     IIPSLMDSIIDLLREQRKRTGNVEGGLARGQYHHKKILEAVIQRDPQAARKAMQDHLQ
                     QVREDTYASAKNTD"
BASE COUNT          984 a         1268 c         1053 g         1149 t
ORIGIN      
        1 gcttcgttct cgcgcatgcc aggtttcatc gcccggtaca gctcatcata agccgcatcg
       61 acctgggccg cggcgtgagt gagcagggca atttcatcct gcgtcttgat gacgcgcgcc
      121 tcagacatca gctgctgacc atcaacgatg gtcagtccct ttttctgcag ctcaaagaga
      181 accggcactt cagcaatatc cacacctacc ggttctttct ctaacccgcg ctctttgagc
      241 tcggcccaga ttttgttcgc cacgtcttca gcgcggccca tttcgggcga catcgccccg
      301 cgcagcaacg agataccagc ccgcgagcgc ttttctccca gccagggagc gtaaagctgg
      361 tggtgtttgg cggcagaccc aaagtcccac aggattggtt cgtcatcctg gggcaacaat
      421 gtaaagcggt tgaccttgtc ctgcgcccaa gtgccgatgt gagtggcagt tatgtagcgc
      481 acgttgttca tatcgaagca gagcagcgcg cccatttccg attctttcag tagcttcttc
      541 gcccggtcga gacgttcacg gcgcaagcgt tcgaagtcta tgcgctgttc ccaatcaacc
      601 tgcatggttc cgtaggtttt tatagccatt tcaatttctc ctcgtttact aggctttgcc
      661 acaaagtgac tttgccattt cgaaaacggt ttcttcagta ggtacggtta aatcttctag
      721 cacaggagag aacgggatgg gtacgttcat ggcgcccatg cgttttactg gtgcatccag
      781 gtaatagaat gcaccatcgg caatcaccga cgcaatctcg gcggtgacgc catattgctc
      841 atagccttcg tcaataacga tggcccgtga agtctttttg gcggattcga tcagcgtttg
      901 tttgtcgagc ggggtggtgg tacggggatc aatgatctcg gcgctgatgc caatgtcttt
      961 gagtttttct gccgctccga gcgccacctg gaccatggaa ctggtcgcca ccaatgtaat
     1021 gtcgtcgcct tgcagcttga tgtctgctac cccgaatggg attgtatatt cctccgctgg
     1081 gacgcgacct ttgatcttgt acatcatttt gtcttcgatg aaaaccacgg ggttctcgtc
     1141 acgtatggcg gtcttgagca aacctttggc atcgtaaggc gtggatggca tggtgacctt
     1201 cagccctggc acatggctga cccaagcctg taagctctgg gaatgctggg cagctgaacg
     1261 gcgcgtggcg cccatggtgg tgcggaacac aatcggaacc ttgagcttgc cgccggacat
     1321 gtaatgaact ttggcagcct ggttgacaat ctggtccatt gcaagggtca ggaaatcgcc
     1381 gaacatgatg tccactaccg ggcgcatgcc cgtcatcgcc gcaccaaccc ccatgccagt
     1441 aaacccaggc tctgagattg gcgagtcgaa cacacgattc ttgccgaact cttccaccag
     1501 accagataaa accttgaacg gcgtgcccgc ttctgctaca tcctcaccga taataaaaac
     1561 attctgatcg cgacgcattt cttcagccag tgcctcgcgg atcgcttctg caaacgttat
     1621 ttcacgctca gccatattta ttgtgctgcc tcccattgtg aaactttaat gtcaggcata
     1681 aacatcctcg gtcacctggc tctgatctgg gtatggcgcg tcgatggcaa actgtacacc
     1741 tgcttccact tcgttttttg catctgcgta aatgcgctca agcacatctt ggtcagtgag
     1801 ctttgatgca actaattttt cctctaacag tttcaacgga tcatggactt ctcgccattc
     1861 ttgctcttct tccttcgagc ggtaatatgt acgattgata tcccccacat ggtgaccgta
     1921 ataacggtag gtcaggcact ctaggaatgc aggtccttca ccgcggcggg cgcgctcaac
     1981 cagtttccgc atcgtctcat agaccagctg cacgtcctga ccgtaaatgc tctcggtgtg
     2041 gatcccaaat gctctggcgc gctcggtaac ctcccctgca agcgcctcgc tataatgggt
     2101 atattcgtta tacaggttat tttcacagac gtagataatg ggcaatttcc acaacgatgc
     2161 catattcatg acttcataca aaagaccttg ccccaatgcc ccctccccga aaaaacaaac
     2221 ggctacctga tccgtgccac gcatcttggc cgataatgct gcgccagtcg ctattccggc
     2281 actgccaccc acaatggcgt tcgcccccag attgccagat tcgggatcag caatgtgcat
     2341 tgagccgcct ttaccgcggc aatacccggc ttctttgccc agaagttcag cgaacatcaa
     2401 gtcaaccgaa gcacctttcg caatacaatg accatgcccg cggtgcgtgc tggtaatgta
     2461 atcgtcccgc cgcaaagctt cacagacccc cacagcgact gcctcttgac ccgaatacaa
     2521 atgggtaagt ccgggcatca gtgccttcat gtataactca ttggccttct cctcgaattc
     2581 acggatcttg gccatctgct cgtaggcatg cagccattgc tcgacgtcta gcgtctctgg
     2641 tttggggttg gtagtgactt gcgtttcatt ggccattact tcactcccga catttgcgcc
     2701 agcacatcga acatgatcgc aaaatcctgt ttttcaagcc ccatacctcg ggctgcagaa
     2761 aggaattcgt tcgttacagc agtggtgggc aggggaatgc tcagctcgcg tcccagttcc
     2821 agtgctaggt tcatatcctt ctgcatcata taacaatcga accaggcttc atctggcatc
     2881 tccaggacga acggaccgcg atacttgacc attggcgaag cgataacact gttaaccagc
     2941 acttctactg caatctcgcg cttgatgccg cttttctcag ccagcagaac accctcgctg
     3001 aaagccagca tttgcactgc cagactgaga ttggttgcga ttttcattgt cacggcgaga
     3061 ccattggcgc caacgtgcgt tactttgggc ccaatatctt gtaaaatcgg ttttgccttt
     3121 tcgaatgtct ctgcgtcacc gccaaccatc acggacaatc gaccctcttc gagagtcacc
     3181 acactgcctg atacaggagc atccagcatg gaagcacctt gtatagcaac ctcctttgcc
     3241 aattcccgac tgaaggccgg actgacggtg ctcatatcta cataaatctt gccagcgctt
     3301 aagcccgcta atagaccatc ggggccttcg gctatgctgt gaagagcttc ggtgtttgtg
     3361 accatactta aaatgatgtc agcagatttg gccacggcac gaggcgtatt accccaggtc
     3421 atgcctgctt ccaacagcca ttgggctttt gaacgagttc ggttatagcc cgttacgctg
     3481 tgaccagcgt ctagaagacg ctttaccatc cgtccaccca ttacaccgag tccgataaat
     3541 cccaggtttg ccatgcaatc ctccgaaatt atgcctcagt ccgtgttttt ggctgaggcg
     3601 taagtatctt cgcgcacttg ttgaaggtgg tcttgcattg ccttgcgggc tgcctgtggg
     3661 tcgcgttgta tgacagcttc aaggatcttt ttatggtgat attgccctcg tgccaggcca
     3721 ccttccacat tccctgtccg cttgcgctgt tcgcggagca aatcaatgat cgaatccatt
     3781 aaggatggaa taattgggtt ttgggttgct tccgccagcg ccagatggaa gtcgaggtcg
     3841 gcctctacaa aaatctcgac gttatccagg gcggtatcca tcgtttcaac ggcctccgtc
     3901 atcgcggtaa tatattcttc gctaatccga gtagcagcca gcgcagcgat ttcgggttcc
     3961 aggatctccc gcacctcttc cagattaaca aagccatttt cagtttttag caacaaacct
     4021 agcgaacgtc gaactgcacc tgccgcacca ctggtcacga atgtcccccg gcctaaacga
     4081 atctccacca gacctttctc tctcaaagcc ttaatggcct cacgcaccgc tgttcggctg
     4141 acagcgaact gttctgcaag ctctcgttca gcaggcaatt gatcccctat cttcagatct
     4201 cccgctaaaa tccggcgttc gatctggtct acaatctgtt catataggcg ttcagactgg
     4261 ataggttgat acattatgag aggtttttca caagtggtat gatgaccata ccattatatc
     4321 acgacaaaaa cgggtgtcaa tagccaaata tggtgaatta tttcacaagc gtatagtgga
     4381 ttctttttag tgaaacacac cgcagaaaac tgcggtgtgt ttcaaaatca tctacttcgg
     4441 taatgccgag cgct
//
LOCUS       DBNHOH010000297         3936 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1748146, whole genome shotgun sequence.
ACCESSION   DBNHOH010000297 DBNHOH010000000
VERSION     DBNHOH010000297.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1748146"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="AC16V7_05320"
     CDS             <1..1051
                     /locus_tag="AC16V7_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HIR6256574.1"
                     /translation="AVRAGDTFGASDSLPAYLQLIGEIPMGGVPGFSLAAAQCALIHT
                     GGMLPEGADAVVMVEHTQSARPGEVEVLRAVAAGENVLKVGEDVLPGDEVIPAGVRIR
                     PAEIGGLMALGMIKTRVSRRPRVGIISSGDEVVSPEKTLGPGQVRDVNAYTLSSLVEA
                     AGGQPVRYGIVSDQEKVMREVGSKALAECDVVVITAGSSASTRDLTSQVVNSLGKPGV
                     LVHGVNVRPGKPTILAVCNNKAVIGLPGNPVSALVIAWLFVIPVVEALLGLAYRQPRP
                     SASARLTLNLSSQAGREDWIPVRLVPGAGGYQADPVFGKSNLIFSLARADGLVRIPAD
                     ATGLEAGEQVEVYLF"
     gene            1051..1449
                     /locus_tag="AC16V7_05325"
     CDS             1051..1449
                     /locus_tag="AC16V7_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR6256575.1"
                     /translation="MELSVLPELLAVCQLSPDEELPPWAGQSDLLAVIRDGDELTIVC
                     AQRFVPPGVQAEQGWRALKISGPLDFSLVGVLAELAATLAQAGVSIFALSTYSTDYIL
                     VKDEVLENAIRALRSSGHLISLVSRLDQAG"
     gene            1483..2478
                     /locus_tag="AC16V7_05330"
     CDS             1483..2478
                     /locus_tag="AC16V7_05330"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR6256576.1"
                     /translation="MPVKYGFLVLLIIIILSSPSWVKEDVSQSPVDDSPQLTGKSITR
                     DMVKELALAKTVLPVEATSTPTVAASALPPTAEPTPTSTPTPQWIWNSPGEVVAPILL
                     YHHVANRDPSSRYFISPDVFEDQMRILHRWGYTTIPLSLLVEALVEGAELPPRPVVIT
                     FDDGYRDVVNHALPIMQHYGFRGVAYAIAGQIGKGKYMKPAELRELFDAGWEIGSHSW
                     SHPNLRSESVSLQREIVESRLKLEELLGSPLETFAFPYGLTSKYITALVKEEGYLAAV
                     GLGTSSRHTEKTRYYLSRIEVRSEYDLQQFKALLPWSGVPDRGERLRNDQQPGNP"
     gene            2522..>3936
                     /locus_tag="AC16V7_05335"
     CDS             2522..>3936
                     /locus_tag="AC16V7_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HIR6256577.1"
                     /translation="MSIYLHDIPLPEAQSRLEQALKEAGRWGVLAAEEIPLDESCLGR
                     VLAEPVWAKISSPHYHAAAMDGFAVRAGDTDNAMPTSPVTLALGAQAVYVDTGDPLPG
                     WANAVIPIENVEPLDAQGEHSPNARRPAAIRIRAALPPWQHVRPLGEDIVATQLVLPA
                     GHVLRPVDLGAIAACGHERVRVARKPRVAILPTGTELVPVGQPVRPGDIIEYNSLVMA
                     SQVNEWGGWATRYPITPDDFDQIRQRVIEAAQGHDLVLLNAGSSAGSEDFSARVVESL
                     GELLVHGVAVRPGHPVILGMIDRGRLAQPAGQETQTKLPIIGVPGYPVSAALTGEIFV
                     KPLIRRWLGLEPDEPLTVRAELTRKVTSPAGDDDYLRVAVGRVGSRLLAAPLARGAGV
                     ITSLVRADGLAILPRGSQGLPSGAEVLVRLYRSPGELERTIFAIGSHDITLDVMAQFL
                     AAKNRRLASANVGSQGGLVALR"
BASE COUNT          725 a         1102 c         1241 g          868 t
ORIGIN      
        1 tgccgtacgc gctggggata catttggtgc cagcgattcc ttgcccgctt atttgcaact
       61 gattggtgag atccctatgg gcggagtgcc gggcttttca ctggctgcag cacagtgcgc
      121 tttgatacat actggcggta tgcttcccga aggggcagat gccgtggtta tggttgagca
      181 tacccagagc gcgcgccctg gcgaagtgga ggtgctgcgc gcggtggctg ctggtgagaa
      241 cgtgctcaag gttggtgaag acgttttgcc cggcgatgag gtgatacctg ctggcgtccg
      301 gatacgccca gctgagatcg ggggcttgat ggcgttaggt atgatcaaaa cgcgggtgtc
      361 tcgtaggccg cgggtcggga tcatttccag tggcgatgag gtggtgtccc cagagaagac
      421 ccttggcccg ggacaggtac gggacgtcaa cgcctacacg ttgagttctc tggtcgaagc
      481 ggcaggcggc cagcctgtgc gctatggcat cgtgtctgac caggagaagg tcatgcggga
      541 agttggatcc aaagctttgg ctgaatgcga tgtagtggtg atcacagcag gttcttctgc
      601 cagcacgcgc gacctgacat cccaggtggt caacagcctg ggtaagccag gggtgctggt
      661 gcatggggtc aatgtgcgcc ctggcaagcc gacgatctta gcggtatgca acaataaagc
      721 agtcatcgga ttgcctggca acccagtgag cgccctggtg atcgcctggt tgtttgtgat
      781 acccgtggtt gaggcgctgc tcggacttgc atatcgacaa ccacgcccca gcgcttcagc
      841 ccgcttgaca ctaaacctct cctcccaggc aggccgggaa gattggatac ccgtgcggtt
      901 ggtccctggc gctggcggct atcaagctga cccggtcttt ggcaagagta atttgatctt
      961 ctcgctggca cgcgccgatg gattggtgcg tattcctgcg gatgccaccg gcttagaagc
     1021 tggagaacag gtcgaggtgt atctcttctg atggagctga gcgtactgcc tgaactgctg
     1081 gctgtgtgcc agctttcgcc ggacgaggag ttgcccccat gggctgggca gagcgatttg
     1141 ctggccgtga tacgcgatgg cgatgagctg actatagtgt gtgcccagcg gtttgtacca
     1201 ccgggggttc aggctgagca ggggtggcgc gccctgaaaa tcagcggtcc gctggatttt
     1261 tccttggttg gtgtgctggc ggaactggca gccacgttag cgcaggctgg ggtgtccatc
     1321 tttgctcttt ccacctacag caccgattac atcctggtca aagatgaggt gctggaaaat
     1381 gccatacgag ccttgcgctc cagcgggcat ttgatcagct tggtttcgcg cctggatcag
     1441 gcaggttgag agcgcttttt cgcctttggg aagttcgatt ttatgcctgt aaaatatggc
     1501 ttcttggtcc ttctgatcat cattatcctc tcttcgccta gctgggtgaa agaagatgtt
     1561 tcgcaaagcc ctgtggacga ttcccctcag ctcaccggaa aatccatcac cagggatatg
     1621 gtgaaagaac ttgcattagc caaaacggtt ttgcctgtag aagccacatc tacgccaacg
     1681 gtcgcggcgt ctgcattgcc accgaccgct gagccaacgc ctacatcgac ccccacgccc
     1741 cagtggattt ggaactcgcc cggagaggtt gtcgccccca tcctgctcta ccaccatgtc
     1801 gctaatcggg acccgtcttc tcgatacttc atatcccccg atgtcttcga ggatcagatg
     1861 agaattttgc accgttgggg ctatacgacg atacctctat ccttgctggt ggaggcgctg
     1921 gttgaaggcg ctgagctccc ccccaggccg gtggtgatca ccttcgatga cggttatcgc
     1981 gatgtggtga accacgccct gccgatcatg cagcactacg gattccgtgg ggttgcttat
     2041 gccatagcag gccagatcgg caaaggcaaa tatatgaagc ccgcagagct gcgcgagctc
     2101 tttgacgccg gctgggagat tggcagccat agctggtcgc accccaacct gcgtagcgag
     2161 tcggtgagcc tgcaacgcga gattgtcgaa tcgcgcctga agctggaaga gttgcttggc
     2221 tcgccgctgg agacgttcgc attcccctat ggcctgacct ccaaatacat caccgccttg
     2281 gtcaaagagg agggctatct ggcagctgta ggattgggga cttcatcccg ccataccgaa
     2341 aaaacgcgtt attatttgag ccgcatcgag gtgcgcagcg agtatgatct ccaacaattc
     2401 aaggccttgt tgccatggtc gggcgtcccg gatcgcggtg aacgcttgag aaacgaccag
     2461 caaccaggca atccgtaatc agatgttgtt attaacccct ttaatgtatt ggacctaagc
     2521 gatgtcaata taccttcatg atatcccact ccctgaagct cagtcacgcc tggagcaggc
     2581 tctcaaagag gccgggcgct ggggtgtgct tgctgctgag gagatccctc tggatgagag
     2641 ctgcctgggg cgcgtgctgg ctgagccagt gtgggcgaag atctcttcac cgcactatca
     2701 cgcggcggcg atggatggtt ttgctgttcg cgctggggat accgacaacg ccatgcccac
     2761 ttcaccagtg acgctggcgc tgggcgctca ggcagtctac gtggacaccg gcgatcctct
     2821 gcctggttgg gctaacgcgg tgatcccgat tgagaatgtc gagcctttgg atgcacaggg
     2881 ggagcattcc ccaaatgctc gccgcccggc tgctattcgc attcgcgccg cgctgccacc
     2941 ttggcagcac gtgcgcccct tgggggaaga tattgtcgct acccaattgg tattgcctgc
     3001 cgggcatgtg ctccgcccgg tggacctggg tgccattgca gcttgcgggc acgagcgcgt
     3061 gcgcgtggcg cgcaaaccgc gtgtcgccat cttgcccacc ggcacagagc tggtgccggt
     3121 aggccagccg gtgcgccctg gcgatattat cgagtataac tccctggtga tggcatccca
     3181 ggtcaatgag tggggagggt gggcgacgcg ctacccgata acacccgacg atttcgacca
     3241 aatccgccaa agggtaatcg aggcagctca ggggcatgac ctggttctcc tgaatgccgg
     3301 ctcctccgca ggctcggagg acttctccgc ccgggttgtc gaatctctgg gggagctgct
     3361 ggtgcatggc gtggcagtgc gcccagggca cccggtgatc ttaggcatga tcgatagggg
     3421 tcgcctcgca cagcctgctg ggcaagagac gcagacaaaa ctacccatta tcggcgtgcc
     3481 ggggtatcct gtatcggcgg ctttaacggg tgaaatcttc gtcaagccgc tgatcaggcg
     3541 ctggttgggg ttagagcccg acgagcccct gactgtacgc gcagagctga cccgcaaagt
     3601 gacctccccg gcaggcgatg atgactacct gcgcgttgcg gtgggtcgtg tggggtcgcg
     3661 attgctggct gcgcccctgg cgcgcggggc gggtgtgatc acttcgctgg tgagagccga
     3721 tggcctggct attctgccgc gcggctcgca aggcctgccc tccggggcgg aggtgctggt
     3781 gcggctctac cgctcccctg gcgaattgga gcgcaccatc ttcgccattg gctcccacga
     3841 catcaccctg gatgtgatgg cgcaattcct ggctgcgaaa aaccgccgcc tggcatctgc
     3901 caatgtgggc agccagggag gtctggtggc attgcg
//
LOCUS       DBNHOH010000298         3086 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_658411, whole genome shotgun sequence.
ACCESSION   DBNHOH010000298 DBNHOH010000000
VERSION     DBNHOH010000298.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_658411"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="AC16V7_05340"
     CDS             complement(<1..590)
                     /locus_tag="AC16V7_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HIR6256578.1"
                     /translation="MIDIGVLTAGFVIILVLDFITVASGTALQKTNMARLLAYSEAGD
                     PGVQGAMRLLNSPARLQATIDLAQWIWRALQVLLLFIILYQGPWISPLAAAAGLTLLA
                     SLVIFWLEWYIRESTGKSAEAWTLRLYPFIAVLLAVFSPFVALPLAVSGGEQEQENGG
                     SMVDELKSLVDAGQRDGLLEQEERRMLRSIFELGNTL"
     gene            complement(597..1943)
                     /gene="glmU"
                     /locus_tag="AC16V7_05345"
     CDS             complement(597..1943)
                     /gene="glmU"
                     /locus_tag="AC16V7_05345"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HIR6256579.1"
                     /translation="MNISAVILAAGQGTRMRSSMPKVLHPLLGSPMLLYSLEAARLVT
                     GTTPVLVIGHGGEKVKQVIGGAAHYALQEPQLGTAHALMQAEPLLRGKADLVLLTAAD
                     MPLITPETLSKLVQAQAAHAGPITLLSGRADTASDFGRVLRGPDGRIQAVVEVAQATP
                     EQLAVREVNTSIYCFAAEWLWDALHRIPLSPKGEYYLTDAISLAVAEGLSVQGVQVED
                     SIETLGINTRVQLAEAEVALRQRINRGWMLDGVTIVDPATTYIEPGVHIGTDTTIWPN
                     TYLQGNTEIGENSVIGPNAVLRNTRVGDGCKVLASVLEDAVLEDDVDVGPFGHLRRGA
                     HLAQGVHMGNFGEVKDSYLGPGTKMGHFSYVGNATIGPKVNIGAGTVTCNYDGEKKSP
                     TEIGAGAFIGSDTMLVAPIKIGEGARTGAGAVVTKDVPPNTLAVGMPARRIKKLRK"
     gene            complement(2091..2161)
                     /locus_tag="AC16V7_05350"
     tRNA            complement(2091..2161)
                     /locus_tag="AC16V7_05350"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2127..2129),aa:Gln,seq:ttg)
     gene            complement(2229..>3086)
                     /locus_tag="AC16V7_05355"
     CDS             complement(2229..>3086)
                     /locus_tag="AC16V7_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256580.1"
                     /translation="GMSGLELLQRMRQNRPELKGILTAVNPDTRTGQLALKANANALV
                     PKPIDISKFLEAVYRCLDQEPERGLPPKQQATSAGSLSERLEAIYHRCGAMAILLLDE
                     DGHILAQAGEAEKAKPLMSQIPVVLEALGASARLSQAMGENIPDDLVCCAGLGVELFA
                     AHLGKLNALLYIFDTGVLGYTRARSVRLVSESVLEMTGFMPMFGGAQRSMLAKKEEPT
                     DPRPKKEAETNVDLDEVFQQASKNSLDPQDVDGFWETAVDQPDSDSGAGPDSISYDQA
                     KDMGLVKDG"
BASE COUNT          741 a          918 c          756 g          671 t
ORIGIN      
        1 agggtgttgc ccagctcgaa gatggagcga agcatgcgac gctcttcctg ctccagcagg
       61 ccatcgcgct gacccgcgtc taccaaggac ttaagctcat ccaccatgct acctccgttt
      121 tcctgctcct gctcccctcc agagactgca agcggtagag ccacaaacgg cgaaaacaca
      181 gctagcagca cggcgatgaa tggatatagc cgaagcgtcc aggcttcagc cgatttgcca
      241 gtcgattctc tgatgtacca ttccagccag aagatcacca gagaggccag caaggtgagc
      301 ccagcggccg cggccagggg tgaaatccaa ggtccttgat aaaggataat gaacagcagc
      361 agtacctgca gggcacgcca aatccattga gcaagatcaa tggtggcttg tagtcgcgct
      421 ggagagttca gcaagcgcat agcaccctga actccgggat cacccgcctc gctatatgcc
      481 agcaggcgcg ccatattggt tttctgcaag gcagtcccag aagctaccgt gatgaagtcc
      541 agcactaata taatgacaaa accagcagtg agcactccaa tatcaatcac aattccctac
      601 ttccttaact ttttgatcct gcgagccggc atacccaccg ccagcgtatt gggcggcaca
      661 tctttggtga ccaccgcacc agcgccagtg cgcgcccctt ccccaatctt aatcggtgcc
      721 actaacatgg tatcactgcc gataaaggcg ccggcaccga tctcagtcgg actcttcttc
      781 tcgccatcgt agttgcaggt gaccgtcccc gcaccgatgt tcacctttgg gccaatggtg
      841 gcgttgccaa cataagaaaa atgccccatc ttggttcccg gacccaggta tgaatccttc
      901 acctccccga aattgcccat atgcacaccc tgagctaggt gagcacccct gcgcagatga
      961 ccgaatggac ccacatcgac atcatcttcg aggactgcat cctccagaac cgaggccagc
     1021 actttgcagc catcaccaac ccgggtgttg cgcaatacag catttggccc aatcacgctg
     1081 ttctcaccga tctctgtatt gccctgaagg taagtatttg gccagatcgt agtatctgtt
     1141 ccaatatgca cgcccggctc aatataggta gttgcagggt cgacgatggt aaccccatcc
     1201 agcatccaac cccggttaat acgttggcgc aaggcgactt ccgcttctgc caattgtacc
     1261 cgggtgttga ttccgagggt ctcgatgctg tcttcaacct gcactccttg tacgctcaat
     1321 ccttctgcta cagccaggct aatcgcgtcg gtgagatagt attctccttt gggggagagc
     1381 gggatacgat gaagggcatc ccaaagccac tcagcagcaa agcagtagat gctggtattt
     1441 acctcgcgaa ccgcaagctg ctcaggtgta gcctgcgcca cttctacaac cgcttggatg
     1501 cgcccatctg gcccgcgcaa tacccgacca aagtcgctgg ctgtatctgc tcgtcctgag
     1561 agcaaagtaa tggggcctgc atgagccgcc tgcgcttgaa cgagcttgct caacgtctcg
     1621 ggagtaatta ggggcatatc ggcggcagtc aacaatacca ggtcagcttt gccacgcagg
     1681 agaggctcag cctgcatcag agcatgcgcg gtgcccaatt gcggttcctg taaagcgtag
     1741 tgcgctgccc caccgatgac ctgcttcacc ttttccccac cgtgaccaat aaccagtacg
     1801 ggtgtagtcc ctgtgacaag ccgggcggct tccagcgaat agagcaacat tggactccct
     1861 aataatgggt gaagtacttt gggcatcgac gaacgcatac gcgtcccctg acccgcggcg
     1921 aggatgacag cactgatatt catacgaact ccatgggaat agaataaaaa caacgagcac
     1981 ccacgatcca attatggtag cgcggggctc gctgaaaggt taaaccaaat ctgtggtctg
     2041 aaactgtctc ttcgtcagac acgaaacaaa acctgtgcaa aacgatcaga ctggggcgcc
     2101 cggattcgaa ccaggatata cggattcaaa gtccgctgtg ctaccattgc accacgcccc
     2161 aaaatcacct gggtaacagg cgaagcgtat tgtatcataa gttagagatg ccaatgtccc
     2221 gaatcagctt acccatcctt aacgagaccc atgtccttgg cttgatcgta ggagatcgaa
     2281 tcgggtccag cacccgaatc gctatccggt tgatccaccg cggtttccca aaagccatcc
     2341 acatcttgtg gatcgaggct attcttggat gcctgttgga agacctcatc aagatctacg
     2401 ttggtctctg cttccttttt aggccgggga tcagtgggtt cttccttctt cgccagcatt
     2461 gaccgttgtg cacctccgaa cattggcata aaaccggtca tctccaagac tgactcgctc
     2521 accagacgaa ccgagcgagc gcgtgtgtaa cccagcacgc ccgtatcaaa aatatacaat
     2581 aacgcattca actttcccaa atgcgcagca aacagctcaa cacctagacc ggcacaacat
     2641 accaagtcat ccgggatgtt ctcacccatg gcttgagata gccgggcgct ggcgcccagg
     2701 gcttccagca caacggggat ttgcgacatt aacggcttgg ctttttctgc ttctccagcc
     2761 tgggccagga tatgaccatc ctcatccagc aagaggattg ccatcgcacc gcatctatga
     2821 tagatagcct cgagtctttc agaaaggctg ccagcagagg tagcctgctg ctttggcggc
     2881 aatcctctct cgggctcttg atccagacaa cggtacacag cttccaaaaa cttggaaatg
     2941 tctattggtt tgggtaccaa agcgttggcg tttgccttaa gcgcaagctg accggtgcga
     3001 gtatccgggt tcaccgctgt caagatgccc ttgagttccg ggcgattttg ccgcattctt
     3061 tgcaacaatt ccaggccaga catacc
//
LOCUS       DBNHOH010000299         2398 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_17449, whole genome shotgun sequence.
ACCESSION   DBNHOH010000299 DBNHOH010000000
VERSION     DBNHOH010000299.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_17449"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="AC16V7_05360"
     CDS             complement(<1..331)
                     /locus_tag="AC16V7_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256581.1"
                     /translation="MNVGQWIVIGLCIFIGLWYATGFYYNRRKSKAVAAWLYTGLSLY
                     GNVKVSRLKGTAAWGAQFHVDCREAPFRYFEVAFLLEARENLPLWIYNRLLRKAHSSS
                     SPASVPAA"
     gene            419..1210
                     /locus_tag="AC16V7_05365"
     CDS             419..1210
                     /locus_tag="AC16V7_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012407398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6256582.1"
                     /translation="MDLGLTNKRALVTGSSRGLGYETARLLAMDGCRVVINSRSAENL
                     FTAAKKLEEETGGQVFPLVGDVTDRALPEQLVQKSAEALGGLDLLVTNSGGPPSGKFE
                     IFDDATWQKAVELNFLSHVRLIRAALPYLRQSKAASVLAITSYSVKQPIPNLVLSNSV
                     RAATVGLIKTLALELGGDGIRFNSILPAWTETERVIELMAFRAQTNGTTVEEEIAKQA
                     KDSPIGRMGHPEEFANAAVFLLSPAASYITGVMLTVDGGMFKGTL"
     gene            1314..>2398
                     /locus_tag="AC16V7_05370"
     CDS             1314..>2398
                     /locus_tag="AC16V7_05370"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HIR6256583.1"
                     /translation="MSAILNIAGSIARQGLTWLGRRRLPQITGNIDIPGLHAPAEIIR
                     DRWGIPHLYANDRHDLFFAQGFVHAQDRLFQMELNRRTSSGCLSEIFGPLALDTDRAS
                     RTFGFARLGRIDWENANDELRNVLLAYTEGVNAYLNHPDSKLPVEFTLLGYHPELWKP
                     EDSSALARFMMWQLSHAWYGEIVRAQLIQAVGAERAAELEIHIPSQNPITLPRGIEFN
                     RLLPGEGLQQVGGPFLKRGMGSNSWSISASRTENRHTYLCNDMHMPLSQPSLWYGNHL
                     VAGDFNVTGVGLPGVPMVLVGHNARIAWGMTLAYTDCEDLFIEQFDTQNPHRYRFRDD
                     WLEAEIIPETIKVKGRPEGHMERVVIT"
BASE COUNT          586 a          652 c          628 g          532 t
ORIGIN      
        1 acgctgcagg aacagatgca ggagatgatg acgagtgggc ctttcgcaac aggcgattgt
       61 aaatccagag cggcaggttt tcgcgtgctt ccaatagaaa tgctacctcg aaataccgga
      121 aaggagcttc tctacaatca acgtgaaatt gcgctcccca ggcagcagtg cctttcaagc
      181 gagatacctt cacgttacca tacagactaa ggccggtata caaccatgct gcaaccgctt
      241 tagactttct gcggttataa taaaatccgg tcgcgtacca tagcccaata aaaatgcaaa
      301 ggccgatgac aatccattga ccaacattca tgcgaacctc ccgcgtgatt atttctccag
      361 tttagcccaa gaggggctca atgtaaagat tctataaatc taatgaggag agaactttat
      421 ggatctggga ttaacgaata aacgcgccct cgtgacagga tcaagtcgcg gtctgggcta
      481 tgaaaccgcg cgcctcctgg caatggacgg ttgccgggtg gtcatcaata gccgcagtgc
      541 ggagaacctg ttcacggcgg caaagaagct tgaagaagag accgggggtc aggtctttcc
      601 actggtcggt gatgtaaccg atcgagccct accggaacaa cttgtgcaga agtctgcgga
      661 ggcgctcggt ggcttggact tactggtcac caattctggc ggacctccat ccggcaaatt
      721 cgaaattttt gatgacgcaa cgtggcagaa agccgtagaa ttgaactttc tcagccatgt
      781 gcgcctgatc cgcgccgctc tgccctacct gcggcagagt aaagcagcca gcgtgcttgc
      841 catcacttcc tattcggtca aacaacctat ccccaacctg gtgctctcca atagcgtgcg
      901 ggctgccact gtcggcctta ttaagaccct cgcccttgag ttaggggggg atgggatccg
      961 cttcaactcc atcctgcctg cctggaccga gacagaacgt gttattgagc taatggcttt
     1021 tcgcgcccag acgaacggta ccacggtcga ggaagagatc gccaaacaag ccaaagatag
     1081 cccaataggg cgcatgggcc accccgagga attcgctaac gccgccgttt tcctgctctc
     1141 gccggcagct tcctatatta ccggcgtgat gttgactgtg gatgggggaa tgttcaaagg
     1201 gacactatag ctagtcatac aagcaatctt atctggctgg tctgagcata tagaccagcc
     1261 agattctttc agcctaagct gcttatctag tagatagaat acgaggtcac catatgtctg
     1321 ccatattaaa tatcgctgga tctatcgctc gccaagggtt aacctggctg gggcgcaggc
     1381 gcttgccaca gatcacaggt aatattgata tccccgggtt gcatgcgccg gcagagatca
     1441 tccgcgatcg ttggggcata ccccacctat acgccaatga cagacacgac ctgttcttcg
     1501 cccaaggctt tgtccacgca caggatcgcc tgttccagat ggaattgaac agacgcacgt
     1561 caagcggatg cctgagtgaa atttttggtc cgctcgcgct ggatactgac agggcatccc
     1621 gcactttcgg cttcgctcgc ctgggacgca tcgattggga gaacgcaaat gatgaattgc
     1681 gcaacgttct cctggcctac accgaagggg tgaacgccta cctgaaccac ccggatagca
     1741 agctacccgt cgagtttacc ttgctcggct accatccgga actatggaag cccgaggatt
     1801 cgtccgcact ggcgcggttc atgatgtggc agctttcgca cgcctggtac ggagagattg
     1861 tgcgagcaca actaatccag gctgtaggag cggagcgcgc agccgagctt gaaatacaca
     1921 ttccttcaca aaatcccatc acactaccac gtggcattga atttaaccgc ctgctgcctg
     1981 gagagggatt gcagcaagta ggggggccat tcctcaaacg cggcatgggt agcaatagct
     2041 ggtctatatc ggcatcgcgg actgaaaatc gccacaccta cctgtgcaat gatatgcaca
     2101 tgccgctatc acaaccttcc ttgtggtatg gaaatcattt agtcgcgggt gatttcaatg
     2161 tcactggcgt tggcttgcca ggcgtcccta tggtactcgt tggacataac gctcgcatcg
     2221 cctgggggat gacgttggct tataccgatt gtgaggacct ctttatcgag caattcgaca
     2281 cccagaaccc ccatcgctat cgattccgtg acgattggct cgaagccgag atcatccctg
     2341 agaccatcaa ggtcaaaggt cgcccggaag ggcacatgga gcgggtagta ataaccca
//
LOCUS       DBNHOH010000300         2383 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_401858, whole genome shotgun sequence.
ACCESSION   DBNHOH010000300 DBNHOH010000000
VERSION     DBNHOH010000300.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_401858"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="AC16V7_05375"
     CDS             complement(<1..579)
                     /locus_tag="AC16V7_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256584.1"
                     /translation="MIASSFREDVLRALEHIRYLSVTIGGRGSCTAAERQAAQYTVDQ
                     MDEMGVKNAHLEFYNGSASTYRPYAMAFGAALLGTLLAWLGEGRVWLALAALLNVLGV
                     LGMLAETDFASNWTRWFSPRASSQNAVGVIPASIQPRRRAVLCAHVDSHRTPIFYSSP
                     TWRKLFQLLVSTAYLNMAVGGAFFALGALLGIT"
     gene            complement(576..1652)
                     /locus_tag="AC16V7_05380"
     CDS             complement(576..1652)
                     /locus_tag="AC16V7_05380"
                     /inference="COORDINATES: protein motif:HMM:NF024247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6256585.1"
                     /translation="MSQNPYQLLAQRLDSLPNGFPPTDDGVELRLLAELFTPQEAMLA
                     AQLRLSRETPEQIAARVGANPQLLREQLKSMARRGLIKAGRVEGGLGYGLMPFVVGIY
                     EMQVERLDAELAQLFEDYYHRAFGQMLSVRPSVHRVVPVGESVHMDIQIHPYESVTDI
                     LAAAQAWGVMDCICRKQKALIGDPCQHPLDVCMILSQKPGAFDQSSFVRALTYDQAQE
                     TLGRAAQAGLVHSVSNSQSGIGYICNCCTCSCGILRGIAELGFANAVAHSAFINQVDE
                     DLCVGCELCVEYCQFDALTMDNIARVDAIRCVGCGVCVPSCPDGALGLVRRPSQEIET
                     TPVDEIAWLQERAAARGLDLGSVL"
     gene            1871..2335
                     /locus_tag="AC16V7_05385"
     CDS             1871..2335
                     /locus_tag="AC16V7_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR6256586.1"
                     /translation="MKLSKRGEYGVRAMIDLASWEGRNGMVQIKDIAEREHIPAKFLE
                     QILLTLKNAGLLQSKMGMGGGYYLAKPADQINLGHIVRVLDGPLAPIRCVSQMAYDSC
                     GCPDEHSCGLRMVMNDVRSAIADVLDRTTLADITKRVEMAHNMPGDTTRPND"
BASE COUNT          578 a          680 c          647 g          478 t
ORIGIN      
        1 ggtgatgccc aggagtgcgc ccagggcgaa aaaagcccca cccactgcca tattaaggta
       61 ggctgtgctc accagcagtt gaaataattt gcgccaggtc ggcgaggaat aaaatattgg
      121 ggtgcgatgg ctgtccacat gggcacacag caccgcgcgc cgacgtggct ggattgaggc
      181 tgggatgacc cccacagcat tttggctgct ggcgcgcggg gagaaccagc gcgtccaatt
      241 cgaggcgaag tcagtctctg ccagcatgcc cagaacaccc aagacattca gcaaagccgc
      301 gagtgccaac cacacacgac cctcgcctag ccaggcaagc aaggtgccca ggagtgcggc
      361 gccaaatgcc atggcgtatg gccggtaggt agaggctgac ccgttataaa attcaaggtg
      421 ggcgtttttc acccccatct catccatttg atccacggta tattgtgccg cctgccgctc
      481 ggctgcggtg cagctccccc ttccgccaat ggtaaccgat aagtaacgga tgtgctccag
      541 cgctcgaaga acatcttctc gaaagctgga tgcgatcaaa gtacgcttcc caggtccagg
      601 ccgcgggcag cggcgcgctc ttgtaaccag gcaatctcat cgactggcgt ggtctcgatt
      661 tcctggctcg ggcggcggac caatcccagg gcgccatccg ggcaggatgg cacgcacacg
      721 ccacagccca cacagcggat tgcatccacg cgtgcgatgt tgtccatcgt caaggcgtca
      781 aactggcaat actcaacaca tagctcacaa ccaacacaca ggtcttcgtc cacctggttt
      841 atgaaggctg agtgtgccac cgcgttggca aaccccagtt cggcaatacc gcgcaggatg
      901 ccacaagaac aggtgcagca attgcagata taaccaatac cactctggct attgctcacc
      961 gagtgcacca gaccagcctg cgcagcccgc cccagcgtct cttgggcttg atcgtaggtt
     1021 aatgcccgca caaaggagct ttggtcgaag gcgcctggct tctggctcag tatcatgcaa
     1081 acatccagcg ggtgctggca tggatcgcca atcaaggctt tctgcttgcg acagatgcaa
     1141 tccatcactc cccaggcttg ggcagcagcc aggatgtctg tcacgctctc ataggggtgt
     1201 atttggatat ccatatgcac gctctcgccc actggtacca cgcggtgtac agatggacgc
     1261 acgctaagca tctgcccgaa agcccggtgg tagtaatctt caaagagttg ggctagttca
     1321 gcgtccaagc gctccacctg catctcgtaa atacccacca cgaagggcat cagaccgtaa
     1381 ccaagacccc cttcaacccg cccggctttg atcaacccgc gccgtgccat cgatttaagc
     1441 tgctcgcgca gcaactgcgg gttggctccg acgcgggcag caatctgttc gggagtctca
     1501 cggctcaggc gaagctgagc agcgagcatg gcttcctgcg gggtgaatag ctcggcaagc
     1561 aggcgcagct cgacgccatc atcagttggt ggaaagccat taggcaagct atccaggcgc
     1621 tgagccagca gttgataggg attttggctc attatctacc tccttcgggt tttcgggcat
     1681 cgtcgtgcaa gcatctaagc aatagacgtt atgccacccg aatagaatac aagaaaaatg
     1741 ccagcaagac aagtgacaaa aatccaattg acaaaagaca aattcgcgta tattctaaga
     1801 atgtaataaa tactatagaa acactttact ttgtaggata ttgttcccct acaatatacg
     1861 agcctaatct atgaaactat ccaaacgtgg tgaatacggt gtgcgagcca tgattgactt
     1921 ggccagctgg gagggccgta atgggatggt gcagatcaag gatatcgccg agcgagagca
     1981 tatcccggcg aaattcctgg agcagatctt gctgacactc aagaacgcag gccttctgca
     2041 aagcaagatg gggatgggag gcgggtacta cctggctaaa cctgccgacc agatcaacct
     2101 gggtcatatt gtgcgcgttc tcgatggccc gctggccccc atccgctgtg tgagccagat
     2161 ggcctatgat tcatgcggct gccctgacga acattcttgc ggcttgcgca tggtcatgaa
     2221 cgatgtgcgc tctgccatcg cagacgtgct tgaccgcacc acgttagccg acatcacaaa
     2281 aagggtggaa atggcacaca acatgcctgg ggacacaacg cggccaaatg actgagtgaa
     2341 ctttactgga aaacccgaaa ggcgcgaggt ggttttctta ttg
//
LOCUS       DBNHOH010000301         2301 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_530217, whole genome shotgun sequence.
ACCESSION   DBNHOH010000301 DBNHOH010000000
VERSION     DBNHOH010000301.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_530217"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="AC16V7_05390"
     CDS             <1..208
                     /locus_tag="AC16V7_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR6256587.1"
                     /translation="DGLDLIQQLIQAAPRWLAPGGLCILEIDIEHAEAALALAQGIFP
                     KANVSIIPDLAGLDRLVSIQLPSE"
     gene            423..1181
                     /locus_tag="AC16V7_05395"
     CDS             423..1181
                     /locus_tag="AC16V7_05395"
                     /inference="COORDINATES: protein motif:HMM:NF016176.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HIR6256588.1"
                     /translation="MRVKILTLSIIALLMAACSAAAPQPEASSPTQAIQPTQPPSAPP
                     TEAPTATTELSPTDIPAAEPSSTEAPSAGNVTAYQIIPGDSQLKYEVGEVFFKDNNRF
                     NLAVGVTPQVTGEILVDMSAPQNSQIGTISADISQFQSDSSRRDNAIRQRFIETSRYP
                     IVTFTPTAVEGLPEAYSEGQEIPLKISGDLTIREVTRPVTFDALVKLEGGTLNGSATT
                     TILMSEFGFGPIDILGTLKTEDEAKVTLSLVARP"
     gene            1296..2084
                     /locus_tag="AC16V7_05400"
     CDS             1296..2084
                     /locus_tag="AC16V7_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018399469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIR6256589.1"
                     /translation="MFLFLSKFLPLLVYPLGLASILILVSLFIRQPRWQKGILALAFV
                     VLYLGGNGWVAHALAKSLEWRYLPPEEIPQADVIVVLAGDERSAQYPRQTPEVGEAGD
                     RVIYAAWLYQQGAAPAILFSGGSIKWLSVGSSPKDAAAYFLEMMGVPRKALWYESESR
                     NTYESAVACRKILDERGIGRVILVTSAAHMPRSVALFEQQGIEVIPAPTDYTVTQSVP
                     QHRLSGRLPSVVISLLPSTEYLEITTRMLKEYFGIFIYKLRGWL"
     gene            2096..>2301
                     /locus_tag="AC16V7_05405"
     CDS             2096..>2301
                     /locus_tag="AC16V7_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HIR6256590.1"
                     /translation="MANPLKDKHIILGVTGSIACYKAVELASKLAQAGALVDVILTQA
                     ATQFVTPLTFQSVTGRRAFVEADL"
BASE COUNT          563 a          639 c          552 g          547 t
ORIGIN      
        1 agatggcctc gacctaatcc agcaattaat ccaagccgct ccgcgatggc tagcaccggg
       61 tggactatgt atcctggaaa ttgatataga gcatgcagag gccgccttag ccctcgccca
      121 gggaatcttc ccaaaggcaa atgtatctat catccccgac ctggcagggc tggaccggct
      181 ggtttcgatc caactcccat ccgaataatc ttcaccccta atcaccaggt aatttaggac
      241 ttacgcagtt gaagaggaaa ggaggggggt ttgggcgggc gatgcccccc ctgccacgta
      301 tccaactgcg taacttctat aattgttaaa gcctgattag aatttctgat cccgcaatcc
      361 atcatgttgg tggatgcgta aaaatagatg attaccaaat ccagaaaaga aagaggaata
      421 caatgcgcgt taagatatta acgttatcaa ttattgcttt gctgatggca gcctgcagcg
      481 ccgcagcccc acaaccagaa gctagtagcc ctacacaggc tattcaaccg acacaaccac
      541 catccgcccc accaacggag gctcccaccg caacaactga gctttcccct acggatatac
      601 cagcagctga accttctagt actgaagcac catcggctgg taatgtaacc gcttaccaga
      661 tcatccctgg agattcacaa ctgaaatacg aggtcggtga agtcttcttt aaagataaca
      721 accgttttaa ccttgccgtt ggggtcacac cccaggttac gggcgagatt ctggtcgata
      781 tgtccgcacc gcagaacagc cagattggca ccatcagcgc cgatatcagc cagtttcagt
      841 cggatagctc aaggcgcgat aacgcaatcc gacaacgatt tatcgaaact tcccgctatc
      901 ctattgtcac cttcaccccg accgcggtcg agggcttgcc cgaggcatat tccgaaggtc
      961 aggaaatccc cttgaaaatt agcggtgatc taaccatccg ggaggtcacc cgcccggtga
     1021 ctttcgacgc cctggtgaaa ctggaaggtg gcaccttgaa tggctccgcc acaaccacca
     1081 ttctaatgag cgagtttgga tttggaccga ttgatatcct cggcactttg aaaacagaag
     1141 acgaagccaa agtaacccta tcgctcgtcg caagaccata aatatctcct agtgcgcgga
     1201 tgtatgtgta tatggggtct gagcaggtgc agggttgccc gcacagaccc cgttttttgc
     1261 tattaattta tctccaaatg cattatgatt aacccatgtt tctttttctt tcaaaatttc
     1321 tgccactcct ggtctaccca ttagggcttg cctctatcct tatcctagtg tctcttttca
     1381 tccgccaacc cagatggcag aaagggattc ttgccctggc ctttgtggtg ctctatctgg
     1441 gtgggaacgg ttgggttgca cacgccttgg caaaatctct agaatggcgc tatttacccc
     1501 ctgaagaaat ccctcaagca gatgtgattg tcgtcctcgc aggggatgag cgttcagcac
     1561 aatacccgcg ccaaactccc gaggtgggtg aggcgggaga tcgcgtcatc tacgctgcgt
     1621 ggctgtacca acagggcgct gctccagcta tcctgttcag cggcggttcc atcaagtggc
     1681 tctctgtggg ctccagtcca aaggatgccg cagcctactt tttggagatg atgggagtcc
     1741 cgcgcaaggc cttgtggtat gagagcgaat cacgcaacac gtatgaaagc gcagttgcct
     1801 gccgtaaaat cctcgatgaa aggggcatag gccgagtgat attggtaacc tctgctgcac
     1861 acatgccccg atcagtagcc ctttttgagc agcaggggat tgaagtcatc ccagcgccta
     1921 ccgattacac cgtcacccag tcagtccctc aacaccgatt atcaggccgg ttgcccagtg
     1981 tggtgatctc gctactcccc agcaccgaat accttgaaat tactactcgc atgctaaagg
     2041 aatatttcgg catctttatt tacaagctac gtggttggtt gtaatataag catatatggc
     2101 taatccactc aaagacaaac acatcattct cggggtcacc gggtcgattg cgtgctataa
     2161 agccgtggag ctggcgtcca agctggccca ggctggtgcg ctggtggatg tcatccttac
     2221 ccaggcagcg actcaattcg tcaccccgct cactttccag tcagtcactg gacgacgcgc
     2281 ttttgtggaa gccgatctat g
//
LOCUS       DBNHOH010000302         7233 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_209871, whole genome shotgun sequence.
ACCESSION   DBNHOH010000302 DBNHOH010000000
VERSION     DBNHOH010000302.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7233)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7233)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7233
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_209871"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /gene="hemW"
                     /locus_tag="AC16V7_05410"
     CDS             complement(<1..967)
                     /gene="hemW"
                     /locus_tag="AC16V7_05410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIR6256591.1"
                     /translation="MIYSTNMDSYSIYLHIPFCKHRCAYCDFNTYAGLDGLIPNYTRA
                     LCREIEYSAASGEPRSVHTVFFGGGTPSLLPAHAIGELLTALDRSFALSVDLEITLEA
                     NPCTLSFEYLNTLRQLGVNRLSLGMQSANAEELRLLEREHDQRDVIQAVSWARRAGFE
                     NLNLDLIFGLPYQQMDNWKHSLTLAVDLHPEHLSLYALTLEHGTPMQKWVGRSLLSEP
                     DADLAAEMYEWAGEFLESKGYGQYEISNWARKDANGKLMVCRHNEQYWRCQPYLGFGA
                     GAHGYAEGFRIANVLSPAVYIERMQPRNSEVLLDFPWTPATTSAVK"
     gene            1104..2258
                     /locus_tag="AC16V7_05415"
     CDS             1104..2258
                     /locus_tag="AC16V7_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR6256592.1"
                     /translation="MSPEQNTKTTKLCPTCGTRLSEDAARCLVCGTELATTDKPSKPA
                     KAVQGSRLPEITLNLPAVLGLFILFIAIGAGLVYYAMRQTTPDTIPPTETPTITITPS
                     PTITTTPQPPTATNTPVPTPTPLSYKVASGDTCSSIAFAFGVSTQSIILLNSLPAACD
                     NLIIGQNLLIPHPTPTSTPQPTATMNAEESTRVACGEFEYTVQENDTLSSISANYAVA
                     IVVLKEYNGLVNDTVRSGQVIKIPLCQRAATAGPSPTPTLPPPYAAPSLLLPQDGAPF
                     TGTDDTVTLQWASVGTLRDNEAYAVTIVDVTGGQDRKLVDYVVDTKYNVPLSFRPTDN
                     LPHVIRWWVLTARQVGTDDSGNPIWEPAGASSAQRDFIWVGVALAVTPTP"
     gene            2539..3234
                     /locus_tag="AC16V7_05420"
     CDS             2539..3234
                     /locus_tag="AC16V7_05420"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR6256593.1"
                     /translation="MSVITISRQYGSGGDEIAELICHNTGYQLFDKHILARAAFEAGI
                     SDQEVIDYSEDNYMVKNFLARLFGISRPIAQLRVWKEGKDGVRVAEEIQLNEEHALSL
                     VRKAIEYAYEIGNIVIMGRGGQVILKDKPDVIHVRIEADIEDRLVRVRSYPEQKTQPF
                     SDSVEARRAAQDIIESFDTGSADYLKRFYGVDSSDPLLYHFIINTSKFSIEHSANLIT
                     QVAQQIKPIAEPA"
     gene            complement(3244..4551)
                     /locus_tag="AC16V7_05425"
     CDS             complement(3244..4551)
                     /locus_tag="AC16V7_05425"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HIR6256594.1"
                     /translation="MDLKEKLAASLPEWRERVRKLVKDSGDVVVGDVTISQVYGGMRD
                     VKALVTDISYVDPNEGIRLRGYTIPEVLEKLPKPEGATMPYVGGLYYLLLVGEIPTRD
                     QAVEVEEEWKRRATVPQFVFYALKAMPIDAHPMMLFSIAILALQNGSLFAKRYHEGMR
                     REEYWEPMLEDSLNLTAKLPAIAAYIYNLKYHERRIIAPDPKLDWAANFAQMVGIPTP
                     EYMDLSRLYFILHSDHESGNASAHATHLAASTLSDIYYAFSAGINSLAGPLHGLANQE
                     SLGWLLHIHDQFTGVPTREQLRQFAWDTLNAGQVIPGYGHAVLRKPDPRFTAQFEYAN
                     KYMPDDEIFQIAKMVYEIVPQVLIEQGKAKNPSPNVDAISGTLQYHYGLKEFDFYTVL
                     FGVGRSLGVSANAVWARALGQPIERPKSLTTAILEDIASKAKD"
     gene            complement(4591..5511)
                     /gene="mdh"
                     /locus_tag="AC16V7_05430"
     CDS             complement(4591..5511)
                     /gene="mdh"
                     /locus_tag="AC16V7_05430"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012660558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HIR6256595.1"
                     /translation="MKSKVSIIGAGMTGSTTAHWLAEREIADIVLVDVVEGMPQGKAL
                     DLQQAMPVIGKDVTVVGSNDYDATAGSDIIVITAGLPRKPGMSRDDLLAANATIVRQA
                     TEKTIEKSPNVIYVVLTNPLDAMAYLTMKTANISPERVVGQAGILDSARMRAFVAMEL
                     GVSVENVHCYVLGGHGDEMVPLTRHSNVAGIPLVDILPSDRLAAIVERTRKGGGEIVS
                     LLKTGSAYYAPAIAVTQMVDAILKDKHLIVDASAYLRGEYGLKDIFFGVPVQLGRKGV
                     EKVIEYRLNEEEMAAFQKSASGVAENLAKL"
     gene            complement(5586..6581)
                     /gene="trxB"
                     /locus_tag="AC16V7_05435"
     CDS             complement(5586..6581)
                     /gene="trxB"
                     /locus_tag="AC16V7_05435"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018461050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HIR6256596.1"
                     /translation="MDFSLTNMASSQQTSPERVKVLIIGSGPAGLSAALYAARADLNP
                     VVLTGMELGGQVSLTYTVENYPGFPDGVGGTELVELFQKQAERFGARLEWDSATEVDL
                     SQRPFRVRTYNGEYLADALVITTGATPIHLDVPGESELTGRGVSYCATCDGWFFKEKD
                     VVIVGGGDSALEEGIFLTRYAKSVSIIHRRDALRAGAILQKRAMENPKIKFIWNSIVT
                     EILGDEAVNAVRLKDVVTGEERDYATDGVFIFIGHRPNTEIFKGQLELNERGYIVADQ
                     RMHTNVPGVFAAGEVADPIFRQVITSAGMGAAAAMQAIHFVEEQEESSAEPKVAA"
     gene            complement(6676..>7233)
                     /locus_tag="AC16V7_05440"
     CDS             complement(6676..>7233)
                     /locus_tag="AC16V7_05440"
                     /inference="COORDINATES: protein motif:HMM:NF013247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR6256597.1"
                     /translation="GEEAQEKGIHMMISSFTRLHPNISMTKAKITGNYINSMLVKTLA
                     LRSGYDEAVILDPQGFVAECTGENIFMIRKGKLYTPPLSTVLDGITRDTIMQLAEDMG
                     IPVVEKMITRDQLYIADEVFISGTAAEIVGVRMIDHRRVGKGGVGPVTQALLHAYHEN
                     LHGNGARSSEWLDLVPGEAGELTDF"
BASE COUNT         1865 a         1994 c         1580 g         1794 t
ORIGIN      
        1 tttttacagc acttgtcgtc gctggagtcc aagggaagtc tagcaacacc tcggagttcc
       61 tgggttgcat gcgttcgata tacaccgcag gcgagagaac gttggctatg cgaaaaccct
      121 cggcatatcc atgcgctcca gccccaaatc ccaaatatgg ctggcatctc cagtattgct
      181 cattgtggcg gcagaccatc agtttcccat ttgcatcctt gcgggcccaa tttgagatct
      241 catattgccc gtatcccttc gactccagga attctcccgc ccattcatac atttctgctg
      301 ccaaatcagc atccggctca cttaacagac ttcgcccaac ccatttctgc atgggtgttc
      361 cgtgctccaa ggttagcgca tacagcgata agtgctcggg gtgcaaatca actgccaaag
      421 tcaggctgtg tttccagtta tccatttgct ggtaaggcag cccgaaaatc aggtccagat
      481 tcagattttc aaaaccagcc cgccgcgccc aagatactgc ttggatgacg tcgcgctggt
      541 cgtgctcacg ctctaaaagt cgcaattcct ccgcattagc ggattgcata cccaagctca
      601 accggtttac acccaactgc cgcaatgtat tcagatattc aaagctgagc gtacatggat
      661 tggcctccaa tgttatctca agatcaactg agagtgcaaa cgaccgatcc aaagcggtca
      721 atagctctcc aatcgcatgc gctggaagca acgatggcgt tccgccacca aaaaacaccg
      781 tatggacgct acgcggttcg cccgaggcag cgctatactc aatttcgcgg cacagcgcgc
      841 gtgtataatt tggaatcaag ccatccaaac ccgcgtaagt gttgaaatcg caataggcac
      901 agcggtgttt gcaaaatggg atgtgaaggt aaatgctgta agaatccatg ttagttgagt
      961 atatcatcct ctgctttccc cgggcgacat gggaaaggct accattaaag aaatactatg
     1021 ccttatcgca catcaaacac aatactggta taatcgccag agtctgcgcc agaacctaat
     1081 agaaaatctg gctggaaatg aatatgagtc ctgaacaaaa cacaaaaacc accaaactct
     1141 gccccacctg cggaacacgc ctgagcgagg atgccgctcg ctgcttggtg tgtggcacag
     1201 aactagcaac cacagataaa ccctctaagc cagctaaggc agttcaaggc agccgtctgc
     1261 cagagatcac cctcaacttg ccagccgtgc tggggctatt catcttattc atcgcaattg
     1321 gtgctggatt agtgtattat gcaatgcgcc aaacaactcc agatacaatt ccgcccacag
     1381 aaacacctac cataacaatc acaccttctc cgaccataac tactactcca caaccgccaa
     1441 ctgcgacgaa tacgcccgta ccaacgccaa cgcctcttag ttacaaggta gcttctggcg
     1501 atacctgttc atcaatcgca ttcgcattcg gcgtctccac gcagagcatt atcctgctca
     1561 acagcttgcc agctgcctgt gacaatctaa ttattgggca aaatctgttg attcctcacc
     1621 ctacacctac cagcacacca cagcccacag ccaccatgaa cgccgaagaa tcgacccgcg
     1681 ttgcttgcgg agaatttgaa tataccgtgc aagaaaacga tacactgagc agtatctccg
     1741 ctaattacgc tgtcgccatc gtagtgctca aggaatacaa cggtctggta aatgacacag
     1801 tccgctctgg ccaggtaatt aaaattccgc tctgccagcg agccgccact gcgggcccgt
     1861 cacccacacc cactcttccg ccaccctatg ctgcgcccag tttactgcta cctcaggatg
     1921 gcgcgccctt tacaggcacc gatgatactg tgacgcttca atgggcttcg gtaggaacac
     1981 tgcgagacaa cgaagcttac gcagttacta ttgtagatgt caccggcggt caggaccgca
     2041 aacttgtgga ttacgttgta gataccaaat acaatgtacc gctttccttc cggcctactg
     2101 acaacctccc ccatgttata cgctggtggg ttctgactgc tcgccaggta ggcaccgatg
     2161 acagcggtaa ccccatttgg gagccagccg gcgcatccag cgctcagcga gacttcattt
     2221 gggtcggcgt cgcgttggca gtcacaccaa caccataatt atttacgagt aatacggttc
     2281 gtgagttagt gtgggtagtc ggcgagattg ttcgactacc cgcatatttt caattgcgat
     2341 aaccccgatt ttgctgaatg cttcttgatt aagcacagca actatgctat attgaattta
     2401 aacaggaacg tggttccagc agcaagccag ttttaggcgg gaaccttaat tccaatgcag
     2461 aatttcatca aagcccgccg tattttatgg cgggctttat cacatatacg aatttaatta
     2521 aggatgagag gttttgccat gtctgttata actatctccc gacaatatgg cagtggcgga
     2581 gatgagatcg ctgaattaat ctgccacaac acaggctacc aactattcga caaacatatt
     2641 ctcgcaagag cggcgttcga agctggcatt tccgatcagg aagttatcga ctactccgaa
     2701 gataactata tggttaagaa cttccttgca cgcctgtttg gtatctctcg cccgatcgcc
     2761 caattacgcg tctggaagga gggcaaggat ggggtgcggg ttgctgagga gatacaactc
     2821 aatgaagagc atgcgctctc gcttgtgagg aaagctatcg aatacgccta cgagatcggc
     2881 aatatcgtta ttatgggtcg tggcggacag gttatcttga aagataaacc ggatgtgatc
     2941 catgtccgca ttgaagccga tattgaagat cgcctcgtgc gggtcagaag ctatccagag
     3001 cagaaaacac aacccttcag cgactcggtc gaagcccgaa gggcagccca ggatatcata
     3061 gaatcatttg acacaggctc ggctgattac ctgaaacgct tttacggtgt cgattcgtcc
     3121 gatccgctgc tatatcactt catcatcaat accagcaagt tcagcataga acactctgcc
     3181 aatctcatca cccaggtcgc gcaacagatt aagcccatcg cagagcctgc ctgagacggc
     3241 gaattagtcc ttcgccttgc tggcaatatc ttctaatata gcagtagtca aagacttagg
     3301 ccgctcaatt ggctggccca gtgcgcgcgc ccatactgca ttagcactta cgcccaacga
     3361 acgtcccaca ccaaacaaca cggtataaaa atcgaactct ttcaagccgt aatgatattg
     3421 aagtgttcca ctgatggcat ctacattggg tgaagggttt ttagctttcc cttgctcaat
     3481 gagcacctga ggcacaatct catataccat tttcgcaatc tggaatatct catcatcagg
     3541 catgtactta ttggcgtatt cgaactgcgc cgtgaagcgt ggatcgggct tgcgtaacac
     3601 cgcatgacca taacccggga ttacctgccc agcattgaga gtatcccaag caaattggcg
     3661 taattgttct cgtgtgggga caccagtaaa ttgatcatga atatgcaaga gccagcctaa
     3721 agattcctgg ttagccaatc cgtgcagtgg tccagccaaa ctattgatac ctgccgagaa
     3781 agcataatag atatccgata gtgttgatgc cgccaaatgt gttgcgtggg cgctggcgtt
     3841 accgctctcg tgatccgagt gaaggataaa gtacaaccgg ctcagatcca tgtattcagg
     3901 ggttgggatg ccaaccattt gagcaaaatt cgccgcccag tccagctttg gatcgggtgc
     3961 gattattcgc cgttcgtggt atttcaaatt atagatatac gccgcaatag cagggagctt
     4021 agctgtcagg tttaggctat cctccagcat gggttcccag tactcttcac gccgcattcc
     4081 ttcatgataa cgcttggcaa aaagcgaccc attttgcagc gcgagaatag cgatagagaa
     4141 aagcatcatg gggtgcgcgt caatcggcat agctttcaga gcatagaata caaattgagg
     4201 gacggtcgca cgtcttttcc attcttcttc tacctcaacc gcctgatccc tggttgggat
     4261 ctctccaacc aatagaaggt agtaaagacc gccaacataa ggcatcgttg cgccttcagg
     4321 cttcggtaac ttttcaagca cttcgggtat cgtataaccc ctcaagcgga tgccttcatt
     4381 cggatcaacg taagagatat cggtgaccag ggcttttaca tcgcgcatac caccatagac
     4441 ttgactaata gtcacatcac caacgacgac atccccacta tctttgacta attttcttac
     4501 ccgctcgcgc cattctggca acgaagctgc taacttttct ttaagatcca tttttttagt
     4561 atcctttgac gaaattctat ttacttaaaa ctacagtttg gcgagatttt ctgctacacc
     4621 actcgccgac ttctggaagg cagccatctc ttcctcgttc aagcgatatt caatcacttt
     4681 ctcaacacct ttgcgtccta actgaactgg cacgccgaaa aagatatcct ttagcccata
     4741 ttcgccccgc aagtaagcag aagcatccac gatcaaatgc ttatccttca ggatcgcgtc
     4801 caccatctgg gttacggcga tggctggggc ataatatgct gatcccgttt ttaataggct
     4861 gacaatctcc ccgccaccct tacgtgtacg ctccacaatt gccgccaaac gatctgaagg
     4921 aaggatatcc actaagggga tgccagccac attcgaatga cgtgtcagcg gtaccatctc
     4981 atcaccatgt ccgcccagca cgtagcagtg tacattttcc acacttaccc ccagctccat
     5041 agctacaaag gcgcgcatgc gcgccgaatc aagaattcct gcctggccaa caacccgttc
     5101 aggtgaaata ttagcagttt tcatggttaa gtaggccatt gcatcgagtg gattggtcag
     5161 caccacatag atcacattgg gcgatttctc aatggttttt tctgtcgctt ggcgcacaat
     5221 tgtagcgttt gctgccagga ggtcgtcccg gctcattccc ggtttgcgcg gcaagccagc
     5281 agtgattact ataatgtctg aacccgctgt agcatcataa tcattgctac ccactacggt
     5341 cacatccttg ccaatcaccg gcattgcctg ttgcaagtcc agcgcctttc cctgaggcat
     5401 gccctccact acatccacca ggacaatgtc agcaatttct cgctccgcca gccaatgggc
     5461 agtggtagac ccagtcatac ctgccccaat aatagaaact ttcgatttca tgcatttctc
     5521 tcctgaataa aataacgatg gttcagaaga tatactcctg aaccactgct taaatcataa
     5581 caaactcatg ccgccacttt tggttcggct gaggattctt cttgttcttc aacaaagtgt
     5641 atcgcttgca tagccgccgc cgcacccata ccggccgacg tgatcacctg ccggaatatg
     5701 gggtcagcta cctcaccggc tgcaaacact cctgggacat tggtatgcat acgctgatca
     5761 gcaacaatat atccacgttc gttcagttcg agttgccctt tgaagatttc agtatttggc
     5821 ctgtgaccta taaatataaa tacgccgtcg gtagcgtaat ctctctcttc gcccgtgaca
     5881 acatctttca acctcaccgc attgaccgct tcatctccca atatctcggt gacaatagaa
     5941 ttccagataa atttgatctt tggattttcc atcgcccgtt tttgtaaaat cgccccagct
     6001 cgtaaagcgt cccggcggtg gattatggag actgattttg catagcgggt caagaagatt
     6061 ccctcttcaa gggcgctatc acctcctcca acgatcacga catccttttc cttgaagaac
     6121 cagccatcgc aggttgcaca gtaagaaaca ccccgcccgg ttagttcgct ctccccaggg
     6181 acatccagat ggatcggagt tgctcccgtt gtgatgacca atgcatccgc caagtactca
     6241 ccattgtagg tgcgaacacg gaaaggacgc tgagagagat ctacttcggt agccgaatcc
     6301 cactccaatc gagctccaaa acgctcggct tgcttctgaa atagctcaac taactcggtt
     6361 ccccccactc catccggaaa accagggtaa ttttcaactg tataggtgag cgatacttgc
     6421 ccacccagtt ccataccagt caatacgacc gggtttagat ccgctcgagc tgcatacaaa
     6481 gccgctgaga gtccagccgg tccagaacca ataattagaa cttttactct ctcagggctg
     6541 gtttgctggc ttgaagccat attcgtcaaa ctaaaatcca tgtatcatat cctcatcgta
     6601 attctatcaa ataatagatg gagaattaca gttgctctca ttttatcaga tgcgccgaca
     6661 agccaaaaag ttgcattaga aatctgttaa ttctcccgct tctccgggga caaggtccag
     6721 ccactcactc gatcgagcgc cgttcccatg aagattctca tgataggcat ggagcaaagc
     6781 ctgggttaca ggcccaactc cacccttacc aactcttcga tggtcgatca tgcgcacccc
     6841 aacaatctcg gctgcggtac ccgaaataaa tacttcatct gcgatatata actgatcgcg
     6901 tgtaatcatc ttttctacta ccggaattcc catatcttcg gcaagttgca tgatggtatc
     6961 gcgagtgata ccatccagaa ctgtggacaa tggcggggtg tataacttcc ctttgcggat
     7021 cataaagata ttttcgcccg tacactcggc cacaaatcct tgcgggtcga ggatgacggc
     7081 ttcatcgtat cccgagcgca acgcaagcgt ctttaccagc atagaattga tatagttgcc
     7141 cgttattttg gctttggtca ttgagatatt gggatgcaag cgggtgaacg aagaaatcat
     7201 catgtggatg cccttctcct gcgcctcttc ccc
//
LOCUS       DBNHOH010000303         2938 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1299552, whole genome shotgun sequence.
ACCESSION   DBNHOH010000303 DBNHOH010000000
VERSION     DBNHOH010000303.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1299552"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="AC16V7_05445"
     CDS             complement(<1..503)
                     /locus_tag="AC16V7_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR6256598.1"
                     /translation="MSHPFDVLPSIPLKPGSSFGPETKPLAHAHRDSPAVTVMTDFRT
                     VTPVTIDTEIPIDRALKKMKTAGVRLLFVTNDAYEIIGLITANDILGERPITYTQQTR
                     MPRSNITVAAIMTPQRGIQVLDAATVQMATVGDIIATLHALERQHALVAKIDEETQTQ
                     TVIGMFST"
     gene            complement(744..>2938)
                     /locus_tag="AC16V7_05450"
     CDS             complement(744..>2938)
                     /locus_tag="AC16V7_05450"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013713.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="spermine/spermidine synthase domain-containing
                     protein"
                     /protein_id="HIR6256599.1"
                     /translation="SAVGCYLIAQQVSFNPEEILWDLNQPLRLLLIYLVLTLPFFFVA
                     NGIGLALTHYHGEINRVYAADLLGASIGSLGIVLLLFFVFPATALQILGTLGFAAAAL
                     ASWELKYQPRRIALVLILLSFTPLMLPAGWLALAISPYKGMNQSLQISGTRIIDEYSS
                     PLGLISVMESSQIPLRHAPGLSLNASGEPPSQLGVFTDADAMTVITAHTDERDKLSYL
                     DQVTSALPYHINKPEKILILGAGGGAEVLQANYHGAGHIDAVELNPQIIDLVREKYKD
                     FAGGLYTAANVDVHIAEARGFVSGSAERYDLIQIALLDSFGASSAGLYSLSENYLYTV
                     QALQEYLARLSPGGMLAISRWIKLPPRDTLKLFATAVETLRQSGIDEPGNRLVLIRGW
                     QTSTLLVKNSPFSEKEILGIKKFCEARSFDTAYYPGITAAETNRYNVLAEPYFFQGAQ
                     VLLSDEAATYLQNYKFNLQPASDDQPYFFHFFKWGALNEILALRGQGGMPLLEWGYLI
                     LIATLIQALIASVVLILLPLLLSRRLLNKKTKNISHSRISGYFIALGLAFLFIEIAFI
                     QKFILFLHHPLYATAMVLASFLLFAGLGSAWSQRYANPQSDKNGVKGAVTGIISVGLL
                     YLLALGPLFSMLNWLPIFVKVIISVLLIAPLAFCMGMPFPLGMARLGKAAPAFIPWVW
                     GVNGCASVLSAVIATILAIHFGFTVVVISALMLYGMAMLVFNGGWGKE"
BASE COUNT          745 a          736 c          778 g          679 t
ORIGIN      
        1 gtggaaaaca taccgatcac cgtttgcgtc tgtgtttcct cgtcgatttt cgccacgagt
       61 gcatgctggc gttcgagtgc atgcagggtt gcaatgatgt cgcctaccgt tgccatttgc
      121 acggtggcgg catcgagcac ctggattcca cgttgcggcg tcatgatcgc ggccaccgtg
      181 atgttggaac gtggcatgcg cgtttgttgc gtatatgtaa tcggccgctc gcctaggatg
      241 tcattggcgg tgatgagccc gataatttcg taagcatcat tggtaacgaa cagcagccgt
      301 acgcccgcgg ttttcatctt cttgagcgcg cggtcgatgg gtatctcggt gtcgattgtc
      361 acaggcgtca ccgttctgaa atcggtcatg accgtgacag caggactgtc gcggtgagca
      421 tgcgcgagcg gtttggtctc ggggccgaac gacgagccgg gttttagcgg aatgcttggc
      481 aacacgtcga acggatgact catcgcgcac ctcctggagc atttattcct tggccactat
      541 gttacgcccc ttgcctgcga gtccaagagg gatgcgcgcg agcgcgctaa tgtcacaata
      601 aaaatcaggt tactagtcct gcttctcgaa ttggcgcggc gcctgggcgg cgagggatgc
      661 cgggggatga tagtgtgtgg ttaagcttta atccctccgt ctcttttaat tttcttattt
      721 cagaaaaagt accagtccat gagctactcc ttaccccacc ctccattaaa aaccaacatc
      781 gccatcccat ataacatcaa cgccgatatc accaccactg taaatccaaa atggatcgca
      841 agaatagtag ctatcaccgc acttaagacc gaggcacagc cattcacgcc ccacacccag
      901 ggaataaaag caggcgcggc cttacccaat cgtgccattc ccaatggaaa tggcataccc
      961 atacaaaagg ccagaggagc aatcagaaga actgaaatga taaccttcac gaaaatcggc
     1021 aaccagttca acatggaaaa taatggcccg agcgccagaa gataaagcag ccctaccgat
     1081 ataattcctg tgacagcccc ctttacgccg tttttatctg actgcggatt ggcataacgc
     1141 tgcgaccagg cgctgccgag ccccgcgaag aggagaaagc tggcaagaac catggctgtg
     1201 gcatagagag gatggtgtag aaatagaatg aatttctgga tgaaggcgat ctcgataaat
     1261 aaaaatgcga gtcccagtgc aataaagtag ccgcttatcc ggctatggct aatattcttt
     1321 gtttttttat tgagtaggcg ccgagataac aacaatggca gcaggatcaa taccacactg
     1381 gcgatgagtg cctggatcaa ggtggcgata agtatcaggt agccccactc cagcaacggc
     1441 atgccgccct ggccgcgcag ggcaagaatt tcatttaatg cgccccattt aaaaaaatgg
     1501 aaaaagtaag gctggtcgtc gctggccggt tgcaggttga atttgtaatt ctgtaaatag
     1561 gttgccgctt catcgctaag caggacctgt gcgccttgga aaaagtaagg ctctgccagg
     1621 acattgtaac gattggtttc agccgccgtg atgccgggat aataggcagt atcaaaggaa
     1681 cgtgcttcgc agaacttttt gatgccaagg atttcttttt cgctgaatgg gctgtttttt
     1741 accagcaagg tactggtttg ccagccacgg atcagtacca gtctattccc gggttcatct
     1801 attcccgatt ggcgtaatgt ctccacggcg gtggcaaaca gtttcaaggt gtcacggggt
     1861 ggcaatttga tccaacggct gatggcaagc attccaccgg gacttaagcg agcgagatat
     1921 tcctgtagcg cttgtacggt atagagatag ttctcgctta aggaatagag ccctgccgaa
     1981 gaggcgccga aggagtccaa taacgcgatc tgtatcaggt cgtagcgctc agcactgccg
     2041 ctgacaaaac ctcgggcttc ggcgatatgc acatccacat ttgcggctgt ataaagtccg
     2101 cccgcgaagt ctttatattt ctcacgcacc aaatcaataa tttgtggatt gagttccacg
     2161 gcatcgatgt gccctgcccc atggtagttt gcttgtaata cttcggcgcc accgccggca
     2221 ccgaggatga gaatcttttc tggtttattt atatggtaag gaagcgcaga ggtgacttgg
     2281 tccagatagg aaagtttgtc tcgctcatcc gtgtgcgccg taatgacagt catcgcatcg
     2341 gcatcggtaa atacaccgag ttgggacggt ggttcaccgc tggcattcag gcttaatccc
     2401 ggtgcatggc gcaagggtat ctgtgaactt tccatcacac tgatcagccc caaggggctg
     2461 gaatactcat cgatgattcg ggtgccactg atttgcaagg attggttcat ccccttgtag
     2521 ggtgagatgg ccagcgccag ccagcccgcg ggtaacatca ggggtgtgaa tgagagcaag
     2581 ataagaacaa gggcgattcg tcgcggctga tattttaatt cccacgaggc caaggcggcg
     2641 gccgcaaagc ctaaagtccc gaggatttgc aatgcagtgg cgggaaagac aaaaaacaac
     2701 aacaggacaa taccgaggct gccgatactg gcgcccagca aatcggccgc atacacacgg
     2761 tttatttcac catgataatg ggtcagcgct aagccaatgc cgttagctac aaaaaagaac
     2821 ggcaatgtca gcaccaaata aatcagcaac agccgtagcg gctgattaag gtcccataat
     2881 atttcctcgg gattaaacga tacttgctgg gcgatcagat aacagccaac cgctgaca
//
LOCUS       DBNHOH010000304         5348 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_81740, whole genome shotgun sequence.
ACCESSION   DBNHOH010000304 DBNHOH010000000
VERSION     DBNHOH010000304.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5348)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5348)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5348
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_81740"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2933
                     /locus_tag="AC16V7_05455"
     CDS             <1..2933
                     /locus_tag="AC16V7_05455"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6256600.1"
                     /translation="FAIITQHLHAPVVPPRAKNDAIPVSLDALVVRLLSKDPDERPAS
                     AALVLEMLDEVASAPSRPQVEAPIELSLLDRIVRGRIVGREGELAEATVLWERARRGE
                     GQLLLVSGEPGVGKTRLVREVVTRAEVSGSLALAGECYAEGGAPFAPFAQIVRGAVQD
                     GSQGLDVPEFILADLLLLAPDLRLYYPDIPPNPSLDPQSDKVRLFENVVAFCQVLCQR
                     TPLLLLLEDVHWADSGTLSLLHYLARRTRRLPLLLVATYRETDLDQTRPLQALLHELN
                     RERLGNQLKLRRLDKTSTENMLAALFAEGITPDFLAGIYQETEGNPFFIEEVCKALVE
                     SGKLAYADGRWYRPEMSDLEIPQSIRVAVQSRASNLPVDAQEVLTLAAVLGRQFDYEL
                     LAAAGRLEENSLIRSLESAVQAQLIEEMSGARGGVFRFTHALIPPILRESLSGPRRRR
                     YHRQVMAALEELRPDDLESLTHHALESGDLQKGYRYSLQAARKAHALYALDEALQHAR
                     HALDATEELDQVDQLVEIYELMGDILFLQGSYLPCAEAYQQALHLTEEPRQRVELNTK
                     IGTTYIWAQDERGLEYLNTVVRDADPRTQSNELAHATALLGRFHHYRGLYRQAIKYLA
                     EAQEIAEPFQDPLVLTSTYAAMAGAYQHLSEFDNSIAWARRLTELGERHNYPLAIALG
                     YEFQAECFTFMGFWSDTLQYGGKNRAIGIKIGAQHRIGWADYSLAVAYFGMGDLSRAE
                     ELAQNSLDLADRIQDNRLQVLSESQLAVILADRGNHQAARTYAKRAQQRGEKLGDISM
                     VGTGNQALAYCYAMNGDWTAAGQLMDAVAVALKRSDTLMVPLLYFGFYSQVTLESGDL
                     EKAEGIAGGNLTLAREAQSPHYEAEALRVLAQLRSVQGQREPAKELLDQAIQIFERLG
                     SRLELGKAFYYYGKAQIEWEEPQAARQSLDRAAHIFEDCQAVHWIQITGDLSSEIKRK
                     GHEG"
     gene            3286..4944
                     /locus_tag="AC16V7_05460"
     CDS             3286..4944
                     /locus_tag="AC16V7_05460"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0004534 - 5'-3' RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HIR6256601.1"
                     /translation="MSKKNNHGGKLRIIPLGGLGEVGKNMMAYQYEENILVVDAGLMF
                     PENDMLGIDYIIPDFEYLIQNRQKIRGVIVTHGHEDHTGAIHHLMQQINAPIYATPLT
                     RGLVEIKLSRGGLLDKIQLNTIQAGEKVQIGPFSVEFFHVCHSIPDGVGLGITTPAGL
                     VIHTGDYKFDHTPVDNWPTDYAKLADFAGRGVLALLADSTNSDKPGWTPSERVIDPAF
                     DQVFREAKGRIIIASFASLISRMQQVANAALRHHRKMAFVGTSMVDNARMARKLGYLD
                     IPDDLLVSVDQALKMSPKDIVLMSTGTQGEPSSIMGRLSTGTNRQFDIIPGDTVVLSS
                     HPIPGNEESVYRTINRLFRRGANVIYEAVAPVHVSGHASQEEMKLMLNLVKPKYFIPI
                     HGELRHLKQHATLAKQVGLPEERIAIVENGQIVEFQNGEMNKGERVPGGYVFVDGAGV
                     GEIGPDIMREREILARDGFVLVNLVLDRNNYRLLEEPEIITRGFVYAPEAEELLEAAR
                     QVVVKTVNIASNGRMQSDLVQTLKSFFYSKTKRRPMVFVTMSES"
     gene            complement(4998..5264)
                     /locus_tag="AC16V7_05465"
     CDS             complement(4998..5264)
                     /locus_tag="AC16V7_05465"
                     /inference="COORDINATES: protein motif:HMM:NF026248.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4491 family protein"
                     /protein_id="HIR6256602.1"
                     /translation="MSLQWAGVILAVVTFATIAAGHVLVRRLHARYSTRPALPLFVIG
                     GLVLIGSLATKDNLLSGALGITAITILWDGIEIYRQEKRMKKNG"
BASE COUNT         1202 a         1445 c         1508 g         1193 t
ORIGIN      
        1 cctttgccat tatcacccag cacctgcacg ccccggtagt cccaccgcgg gcaaagaacg
       61 atgctattcc cgtctctttg gacgctttgg tcgtccggtt gctgagcaag gatccagacg
      121 agcgcccggc atctgctgcg ctggttttgg aaatgctaga tgaagtcgct tcggcgccgt
      181 ctagacccca ggtcgaggcg ccgatagagc tatccctgct cgaccgcatt gtgcgcgggc
      241 gtatcgtggg gcgagaaggc gagctggctg aagcaactgt cctgtgggaa cgcgctcgcc
      301 gtggcgaggg ccagctcctg ctggtcagtg gagagccggg ggtgggcaag acccgcctgg
      361 tgcgcgaagt cgtcacccgc gctgaggtct ctgggagctt ggcactggct ggggaatgtt
      421 acgcggaagg gggcgcgcct tttgccccct ttgcccagat tgttcgcggc gcggtgcagg
      481 atggctcgca ggggctcgat gtgccagaat tcatccttgc cgacctgctc ctcttggctc
      541 ctgatttgcg cctttactac cctgacattc cgcccaatcc atcattggat ccacaatcgg
      601 ataaagtgcg cctttttgaa aatgttgtgg cgttctgcca ggtgctgtgc cagcgcaccc
      661 cgctattgct gttgttggaa gatgtgcact gggccgacag tggcacattg agcctgctcc
      721 actacctggc tcgccggacg cgccgcctgc ccctcctgtt ggttgccacc taccgagaga
      781 ctgatctcga ccagacccgc ccgctgcagg ccttgttgca cgaactgaac cgggagcgat
      841 tgggcaacca gttgaaattg cgccgcctgg acaaaaccag cactgaaaat atgctggcgg
      901 ctctctttgc cgaagggatt acgcctgatt tcctggcagg gatttatcag gagacggaag
      961 gaaacccctt cttcatcgaa gaggtctgca aggcgctggt ggagagtggt aagcttgctt
     1021 atgctgatgg gcgttggtat cgtccggaaa tgagcgatct ggaaatcccg cagagcatac
     1081 gcgtggctgt ccaatccagg gcgagcaacc tgccagtcga tgcacaggaa gtgctaaccc
     1141 tggcagcagt gctggggcgc cagtttgatt acgagttact ggcagcggct gggcgccttg
     1201 aggaaaacag cttgatccgg tccctggaga gcgctgttca agcccaactc atcgaggaga
     1261 tgagcggcgc acgcggtgga gtatttcgtt tcacacatgc gctcattcca cccatcttga
     1321 gagagtcgct gagtggtcca cgccgccgtc gctatcaccg ccaggtgatg gcagccctcg
     1381 aagaactgcg tcccgacgac ctggaaagcc taacccatca tgccttggag agcggcgacc
     1441 tgcagaaagg gtatcgttat tcgcttcagg ccgctagaaa agcccacgcc ctttacgcgc
     1501 tggatgaagc cttgcagcat gcccgccacg ccttggacgc gacggaggag cttgatcagg
     1561 tggatcaact ggttgaaatc tatgagctca tgggtgacat cctctttcta caagggtcgt
     1621 atctgccctg tgctgaggcg taccaacagg cactgcatct caccgaagag cctcggcagc
     1681 gcgttgagtt gaatacaaag attggcacaa cttatatctg ggcccaggat gagcgcgggc
     1741 ttgagtacct gaatactgtc gtccgcgatg ccgatccacg cacccagtcg aatgagttag
     1801 cgcatgcaac tgcattactt ggcaggttcc atcactaccg aggcctgtat cggcaagcta
     1861 taaagtatct ggcggaggcc caagagatcg ccgagccatt ccaagatccg cttgtgctta
     1921 cgtccaccta tgcagcgatg gcgggggcgt accagcactt gagtgagttt gacaacagca
     1981 tagcatgggc gcgtcgtttg actgaattag gcgagcgcca taattatcca ttggcaatcg
     2041 ccctgggtta cgaatttcag gctgaatgtt tcacatttat gggattttgg tcagacaccc
     2101 ttcagtatgg cggtaaaaac cgtgccattg gcatcaagat cggcgcccag catcgcatcg
     2161 gctgggccga ctattccctg gcggttgcat attttggcat gggtgatctg tcgcgcgccg
     2221 aagagctagc tcaaaactcc ttggacctgg ctgatcgcat tcaagataac cggttgcaag
     2281 tgctttccga gagccaactt gcggtcatct tagcagacag gggaaatcat caagctgcga
     2341 ggacatatgc caaacgagcc cagcagcgcg gggaaaagct gggggacata tctatggtcg
     2401 gtacaggcaa tcaagctctt gcttattgct atgcgatgaa cggtgattgg acagcggcag
     2461 gtcagttaat ggatgcggtt gcggttgctc tgaaaagatc cgataccctt atggtacctt
     2521 tgctgtattt tggtttctac agccaggtta cccttgagtc aggtgacctg gagaaagctg
     2581 aaggcatagc tggcggcaat ctgaccctgg cgcgggaggc tcaatcgccc cattatgagg
     2641 cagaggcttt gcgtgtcttg gcgcagctgc gcagcgtgca gggtcagcgc gagccagcca
     2701 aggaattgct cgaccaggcg atccagattt tcgagcgctt aggcagccgc ctggagcttg
     2761 gcaaagcgtt ctactactat ggaaaggcgc aaattgaatg ggaagagcca caggcagccc
     2821 gccaatccct cgatcgagcc gcacatatct ttgaagattg ccaggcagtt cattggattc
     2881 aaataactgg ggatttgagt agcgaaatta aacgcaaagg acacgaagga tgaagaaata
     2941 agttgggcca ttgttgatac ttcgtgttca ttatgttaat aattcataga tattgcccaa
     3001 ttaatctttg acaaaaccta tccatcatgc tacaattctt ttcgttcgtg agtcacgcct
     3061 ggccggtact ccggccccat catgtaccat ggttcaaaca accagaagga atgtgagggg
     3121 aaacatatta cgcattgaat caatgccaat ttgtatattg cgccccggcc ggatgttcac
     3181 agactatcga gtcaacccgc cggggcgttt atattacgac aaatgaacct tccgcaggtt
     3241 cttaacctgg gagaggttaa tacttagtaa ggattataga aagatatgag taaaaagaat
     3301 aatcatggag gtaaactccg cattatcccc ctgggaggcc tgggggaggt cggtaagaac
     3361 atgatggcct accagtacga ggagaatatt ctcgttgtgg acgctggcct gatgttcccc
     3421 gaaaacgata tgttggggat cgactatatc atccccgatt ttgaatattt gatccaaaac
     3481 cgtcaaaaaa ttcggggggt catcgtcacc catgggcacg aggatcacac tggtgccatc
     3541 catcacctga tgcagcagat caacgcaccc atctatgcta caccactgac gcgcggcttg
     3601 gtggaaatca agctctcgcg aggcggcttg ctggataaaa tacaattgaa caccatccag
     3661 gctggcgaga aggtccagat tggccccttc agcgtggagt ttttccacgt ctgccactcc
     3721 atccccgatg gagtgggctt gggcatcacc acccctgctg gtctggtgat ccataccgga
     3781 gactacaagt tcgaccacac cccggtggac aattggccca ccgattacgc caagctggca
     3841 gattttgccg gacgcggcgt gctggctttg cttgccgatt cgaccaattc cgacaaaccc
     3901 ggttggaccc cctccgagcg ggtgatcgac ccggcctttg accaggtttt ccgggaagcc
     3961 aaggggcgca tcatcatcgc cagcttcgcc tcgttgattt cgcgcatgca gcaggttgcc
     4021 aacgccgctt tacgccacca tcggaaaatg gcctttgtag gcaccagcat ggtcgataac
     4081 gcacgcatgg cgcgtaaatt gggctatttg gatatccccg acgatttgct ggtgagcgtg
     4141 gatcaagctc tcaagatgag tccgaaggat attgtcttga tgtccactgg tacccagggt
     4201 gagccgtctt cgatcatggg gcgcctatcc accggcacca atcgccaatt tgacatcatc
     4261 cctggcgata ccgtggtgct ctcgtcacac cctatacccg gcaacgagga gagcgtctat
     4321 cgcacgatta accgtctctt ccgccgcggc gccaatgtga tctatgaagc ggttgcccca
     4381 gtgcatgtct cgggacacgc cagccaggaa gagatgaagc tgatgctcaa cctggtcaaa
     4441 cctaaatatt tcatccccat ccatggggag ctgcgccatc tcaagcagca cgccaccctg
     4501 gccaagcagg taggcctccc cgaagagcgc atcgccatcg tagaaaatgg tcagatcgtt
     4561 gaatttcaaa acggcgagat gaataaaggc gagcgcgtgc ctggcggtta cgtttttgtg
     4621 gatggagccg gcgtgggcga gattggacca gatatcatgc gcgagcgcga gatcctggct
     4681 cgcgatggat ttgtgctggt caacttggtg cttgatcgga ataactaccg cctgcttgaa
     4741 gagccggaga ttatcacgcg tgggtttgtc tatgccccag aagccgagga gctgctggaa
     4801 gctgcccgac aagtggttgt caagacagtc aatatcgcca gcaatggacg catgcagtct
     4861 gacctggtgc aaacgctgaa gtcgtttttt tacagcaaaa ccaagcgccg ccccatggtt
     4921 tttgtgacca tgagcgaatc ataataacaa gctgattgat cgaaaaacgg gtaaatgtag
     4981 gcggtatcgt cgcccattta cccgtttttc ttcattcgtt tctcctggcg gtagatctct
     5041 atcccatccc ataaaatggt aatggcggtg atgcctaaag cgccggagag cagattgtcc
     5101 ttggttgcaa gcgagccgat cagcaccaat cccccaataa caaagagagg cagcgccggg
     5161 cgagtgctgt agcgggcgtg caaccggcgc accaggacgt gtccggctgc gatggtggcg
     5221 aaggtgacca ccgccagtat cacaccagcc cattggaggc tcacgactca accctttcat
     5281 gaggaggaat attgttctct ccgccagttc tccccgctgt tctcttggga ttagccggga
     5341 actgcccg
//
LOCUS       DBNHOH010000305         1308 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1107113, whole genome shotgun sequence.
ACCESSION   DBNHOH010000305 DBNHOH010000000
VERSION     DBNHOH010000305.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1107113"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="AC16V7_05470"
     CDS             <1..637
                     /locus_tag="AC16V7_05470"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013232.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019284 - obsolete L-methionine salvage
                     from S-adenosylmethionine [Evidence IEA];
                     GO_process: GO:0033353 - L-methionine cycle [Evidence
                     IEA];
                     GO_process: GO:0046124 - purine deoxyribonucleoside
                     catabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase family protein"
                     /protein_id="HIR6256603.1"
                     /translation="AIHQMLPGASYEKSPFGEWFVHSNTVPVIIFQGGWGKISAAASA
                     QYAIDRWKPDLLINMGTCGGFEGEIERGTVILAERTAVYDIIEQMDDDTPFLAFYTTE
                     IDLSWLAEPYPMEVKRTLLVSGDRDLLAEEIPTLKERYGAVAGDWESGAIAWVAARNG
                     VRLLILRAVSDLVGSGGGEAYGDYAGFKRAARVIMEKLIASLPTWLERANL"
     gene            774..>1308
                     /locus_tag="AC16V7_05475"
     CDS             774..>1308
                     /locus_tag="AC16V7_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6256604.1"
                     /translation="MVVNNDLSERELEILKLVATGASNKEIARDLFISTNTVKVHLRN
                     IFSKIGAVSRTEAAMYAVQAGVVAGNNSIEGGFTAPIPTTELPGRWRLILPIGIIVII
                     ILLGFLLVQQTQPVQQTSGSDLSQAEQARWQEKAPMPTARFGLAAAVYENQIYAIGGE
                     TDQGVTGAVERYDPQTDA"
BASE COUNT          306 a          317 c          390 g          295 t
ORIGIN      
        1 agccatccac cagatgttgc ctggggcttc ttatgaaaaa tcaccgtttg gagagtggtt
       61 tgtccacagc aacactgtcc ctgtgatcat ttttcagggt ggctggggca aaatttccgc
      121 ggccgcatct gcccaatacg ccatcgaccg ctggaagccg gatttattga tcaatatggg
      181 gacgtgtggt ggtttcgagg gggagatcga gcgcggaacc gtcatcctgg cagaacggac
      241 tgctgtgtac gacatcattg agcagatgga tgacgataca ccgttcctag ccttttacac
      301 cacggagatc gatttatcct ggttggccga gccatatccc atggaagtga agcgaacgct
      361 gctggtatct ggtgaccggg atctgctggc tgaagagata cccacgctca aagagcgtta
      421 tggcgctgtg gcaggcgatt gggagtcggg ggcaattgcc tgggtggcag cccgcaacgg
      481 tgtgcgcctg ctcatcctgc gcgcagtcag cgacttggtc ggcagcggag ggggagaggc
      541 ttatggagat tacgcaggct tcaagcgagc cgcgcgggtg atcatggaga aattgatcgc
      601 ctcgctacct acctggttag aacgagccaa tttatagtcc cttatatcgc tgtgatcaac
      661 gctcaagatc aattaacccc tgcttacagt tttgatggtc gcaaaggcgg gagaagcgat
      721 ccaatttagt ggaatgttat aatttgtaga cccactaaat agggatttct actatggttg
      781 taaacaacga tctgagcgaa cgcgagctag aaatattgaa gcttgttgcc acgggggcaa
      841 gcaataagga gatcgctcga gatttattta tcagcaccaa cacggtaaaa gtccacctgc
      901 gcaatatctt ttccaagatt ggggctgttt cgcgcacgga agcggcgatg tatgcggtgc
      961 aggcaggggt ggtggcgggg aataattcaa ttgagggtgg tttcactgca cccatcccaa
     1021 caacagagct gcctggtcgg tggaggctga ttctccccat tgggattatt gtcatcatta
     1081 tcttgcttgg gtttcttctt gtccagcaga cacaaccggt gcagcagact tccgggagcg
     1141 acctgagtca ggcggaacaa gcacgctggc aggagaaagc ccccatgccc acagcacgtt
     1201 ttggcctggc tgcggcggtg tacgagaacc agatctacgc cattggcggc gagacggacc
     1261 aaggcgtcac cggagcagtg gagaggtacg atccccaaac ggacgcat
//
LOCUS       DBNHOH010000306         3885 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_402165, whole genome shotgun sequence.
ACCESSION   DBNHOH010000306 DBNHOH010000000
VERSION     DBNHOH010000306.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_402165"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="AC16V7_05480"
     CDS             <1..663
                     /locus_tag="AC16V7_05480"
                     /inference="COORDINATES: protein motif:HMM:NF044761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6256605.1"
                     /translation="YAQMTIQDILDDLQISKGAFYHYFDSKGEVLETLVERMVVDEVI
                     PLLTNIVEDPDLPALDKLNRFFSTSVRWKTAKKTFMLALLRVWLADENAIVRQKLFAM
                     SIKQVTPLLTKIIHQGIREGVLATSYPDQVAEVVYSLLQRLGDTFGGLLLSYNPEQGD
                     LRQLEITVAAYTHALERVLGAPSASIVLVDDESLKEWFVTVGDSNVVHGELHTEGNVA
                     FG"
     gene            757..1986
                     /locus_tag="AC16V7_05485"
     CDS             757..1986
                     /locus_tag="AC16V7_05485"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6256606.1"
                     /translation="MRLYLRLAWRNLWRQRRRTFIVVLAMALGLSMMMLYDGLVAGFD
                     QAIYGNAIRILGGNIHVHAAGYRAKADQTPLLPLADDQAVVAAAQAQPQVLVATRRIN
                     TGGLASSREGAFAVGITGIEPEIEKDVNLAAQLVIAGRFLNSDDADVIFIGKGLADAM
                     GVQVGDRITLTGRATHEQMRRRTMTVIGIYDLDMPDIEKRTIYISLSEAQSLYGLNGQ
                     STEVAIELKQLGQEPAVISALQPALPGYEVESWETNFPELKAALGTKGAVMNVFSVII
                     LLIAGIGILNLLLMAVYERTREIGVLGALGLKPRQISLLFVLEGTMMGVVGIAAGIVI
                     GLAINGLLRQVGLDYSQFANVTSYMALVGGRVYPEWGLDKIVGRGLTVAIISALAAFI
                     PAREAAQREPAEALHYV"
     gene            1995..3212
                     /locus_tag="AC16V7_05490"
     CDS             1995..3212
                     /locus_tag="AC16V7_05490"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6256607.1"
                     /translation="MIQLFKMAFRDLGRNRRRSFFSSLALALGLALLLLMAAFIQGEM
                     GSAMDATIRLQSGHLQIRAQSYDESKTSLKWEDLIENPAQVAEQLASLAPVKVATPRL
                     FASGIVSVRDETAGVRIFGIDPLSEANTPYQLGMISGEFLAPDDRQGELVGQPLAEKL
                     GLNVGDKFSLSANTSNGDVAEQVFTIRGIYSTETNAFDSTTVFLPLAKAQTITQAENH
                     ASTIFVLLNDKAQTEPVVAALQTSKYKVLTWTEMNALIIQYEEFANAYMVILYLIVLA
                     ITATVIVNTLVMAVFERTREIGILSAIGMKGGRIMAMFLAESTLLAVGGIIMGLILGN
                     LVVAYFTKNGFYIGNMGITGLLIGNTIYTELTLEDTINLTVIALVITLLAGLYPALLA
                     ARMEPVEALHGGQ"
     gene            3282..>3885
                     /locus_tag="AC16V7_05495"
     CDS             3282..>3885
                     /locus_tag="AC16V7_05495"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6256608.1"
                     /translation="MEVARIENVTRIYKTGKVETHALRGVSLSIESGEFAALVGPSGS
                     GKTTLLQLIGCLDKPTSGRVYVGGKDVSQLNRNQRANMRRGTIGFIFQFFALIPTLTA
                     YENVELPLLLNGHSSSERRARVMQLLESVDLTERAFHRPDQLSGGEQQRVAVARALAT
                     QPTLVLADEPTANLDTPNGKQVMETMTRLNQETGVTFVFAT"
BASE COUNT          852 a         1055 c         1105 g          873 t
ORIGIN      
        1 tacgctcaga tgacgatcca ggacatcctg gatgacctgc agatctccaa gggggcgttt
       61 tatcattatt ttgactcgaa aggggaggtg ctggaaacgc tcgtcgagcg catggtcgtg
      121 gatgaggtca taccactgct caccaatatc gtggaagacc ctgacctgcc tgccctcgat
      181 aaactcaacc gctttttctc cacgtctgtg cgttggaaaa cagccaagaa gaccttcatg
      241 ctggccttgc tgcgagtgtg gctcgccgat gaaaacgcca ttgtacgaca gaaattgttc
      301 gcaatgtcga ttaaacaggt cacgccattg ctaaccaaga tcatccatca gggaatccgg
      361 gaaggtgtcc tggcgacttc ctatccggac caggtagccg aagtggtcta ctcccttcta
      421 caaaggcttg gggacacatt cggcgggttg ctgctctcat ataatcctga gcagggcgat
      481 ctgcggcagc tcgaaatcac cgtagccgcc tatacacatg cgctcgagcg cgtgttgggg
      541 gctccaagtg cttcaattgt gcttgttgac gacgaatcac taaaggaatg gtttgtaact
      601 gtgggtgata gcaacgttgt ccatggcgaa ttgcacactg aaggaaatgt agcatttgga
      661 tgataaaggg cggggttgga tgggttgtcg aagatctggc tgttttttgg tcagacagtc
      721 catccttcgc cgaactaaat gaaaggaatg ctaaacatga gattatatct acgcctggcc
      781 tggcgaaatc tttggcgcca acgccgccgc acgttcattg tggtcctggc tatggccttg
      841 ggactgtcga tgatgatgct ttacgatggt ctggtagccg gtttcgatca ggcgatctat
      901 gggaatgcta tccgcatcct gggaggcaat attcatgtcc atgccgccgg ttatcgcgcc
      961 aaagcggacc aaactccgct cctgccgctg gcggatgacc aggcagttgt ggctgccgcc
     1021 caggcgcagc cgcaggtcct ggtagcaacc cggcgcatca acaccggcgg gttggcaagc
     1081 agccgcgagg gcgcattcgc agtgggcatc acaggcatcg agcctgagat agagaaagat
     1141 gtcaatcttg ccgcgcagct ggtgatcgcc gggcgtttcc tgaactccga tgacgcggat
     1201 gtgatattca ttggtaaagg actggccgat gccatgggcg ttcaggtagg tgaccgtatc
     1261 acgctgaccg ggcgggctac gcacgagcaa atgcgtcgcc gaaccatgac cgtcattggc
     1321 atttatgact tggacatgcc cgacatcgag aaacgcacca tctacatctc cctgagcgaa
     1381 gcgcaatctc tctatggctt aaacgggcag tccaccgagg tggcgatcga gctgaaacaa
     1441 cttgggcagg agcccgccgt aatctctgcg ttgcaaccgg cattgcctgg ttacgaagtt
     1501 gagtcctggg agaccaattt ccccgagctc aaggccgccc tcggaaccaa aggtgctgtc
     1561 atgaacgtgt ttagcgtcat aatcctgctg attgccggga taggcatcct caacctgctg
     1621 ctgatggctg tctacgagcg cacgcgcgag atcggggtgc tgggcgccct cgggctcaag
     1681 ccacgccaga tttctcttct gtttgtcttg gaaggcacga tgatgggagt ggtcggcata
     1741 gcggctggga ttgtcattgg ccttgccatt aatggcctcc tcaggcaggt tggacttgat
     1801 tacagccagt tcgccaatgt caccagttac atggcgttgg tcggcgggcg cgtatatcct
     1861 gagtggggcc ttgataagat cgtcgggcga gggctaaccg tggcgatcat ctcggcgctg
     1921 gctgccttca tcccggcgcg tgaggcggcc caacgtgagc ccgctgaagc tctgcattac
     1981 gtgtgaggtt ggcgatgata caactcttta aaatggcttt ccgcgacctc gggcgcaacc
     2041 gtcgccgttc attcttttca tccctggcgc tggctctcgg cctggctctg ctgctgctga
     2101 tggcggcctt catccagggt gagatgggca gcgctatgga tgcaaccatc cggctgcaaa
     2161 gcggtcatct gcagatacgg gcgcaatctt acgatgagag caagaccagc ctcaagtggg
     2221 aggacctgat cgaaaaccca gctcaagttg ctgagcagct cgcctcgctg gctccggtga
     2281 aagtagccac acccaggctt tttgccagcg ggatcgtctc tgtccgcgat gaaacagcag
     2341 gggtgcgaat cttcggcatt gacccgctct ctgaagccaa tacaccttac cagttaggaa
     2401 tgatcagtgg tgaatttctg gcacccgatg atcgccaagg cgagctcgta gggcaaccgc
     2461 tggcagaaaa actaggattg aatgttggcg acaagttcag tctctcggca aatacctcga
     2521 acggcgatgt ggctgaacag gtttttacca tacggggcat ctactccacg gagaccaatg
     2581 ccttcgacag caccaccgta ttcctgccac ttgccaaagc gcagaccatc acccaggcag
     2641 agaaccacgc cagcacgatc ttcgtcttgt tgaatgacaa ggcacaaacc gaacccgtcg
     2701 tcgccgcgct gcaaacctca aaatataagg tcttgacctg gacagagatg aatgcgctca
     2761 tcatccagta cgaagaattt gccaatgcct atatggtcat tctctacctg attgtgctgg
     2821 ccataacagc caccgtgatt gtgaatacgc tcgtcatggc ggtcttcgag cgcacgcgtg
     2881 agatcggcat cctttcggcg attggcatga agggaggccg catcatggcg atgttcctgg
     2941 ctgaatctac gctgctagcc gtgggcggca tcatcatggg cttgatcctg ggcaatctgg
     3001 tggttgccta ttttacgaaa aatggatttt acatcggcaa tatgggtatt accggcctgc
     3061 tcatcggcaa caccatttac acggagctga ccctggaaga tacgatcaat ttgaccgtca
     3121 tagcgcttgt catcacgctg ctggccgggc tttatccggc cttgctcgct gcgcgcatgg
     3181 agcccgtcga agccctgcac ggcggacaat aatgggagca ggacttacgt agttgaaggg
     3241 gacatcacct ccccaactgc gtaacttcta aggagtataa aatggaagtc gcaagaatcg
     3301 aaaatgtcac gcggatctat aagactggca aggttgaaac ccacgcgctg cgcggcgtca
     3361 gtctttccat tgagagtggt gaatttgctg ctctggttgg cccgtctggt tcggggaaga
     3421 ccaccctctt gcagttgatt gggtgcctgg acaagccaac cagcgggcgc gtctacgtgg
     3481 gcggcaagga tgtcagccag ctcaatcgca atcagcgcgc caacatgcgc cgcggcacga
     3541 ttggttttat ctttcagttc tttgccctaa tcccgacgct gacggcttat gaaaacgtag
     3601 agctgccatt gctcttaaac ggtcacagca gctccgagcg tcgcgcccgg gtcatgcagc
     3661 tgctcgaatc ggttgacctt accgagcgcg ctttccatcg cccagatcag ttgagcggcg
     3721 gggagcagca gcgcgtggct gttgctcgcg ccctggcaac ccaaccaacg ctggttttgg
     3781 cagatgaacc caccgccaac ctggatacgc ccaatggcaa acaggtcatg gaaacaatga
     3841 caaggcttaa tcaggagacc ggcgttactt ttgtttttgc gacgc
//
LOCUS       DBNHOH010000307         1667 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_786827, whole genome shotgun sequence.
ACCESSION   DBNHOH010000307 DBNHOH010000000
VERSION     DBNHOH010000307.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_786827"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..462
                     /locus_tag="AC16V7_05500"
     CDS             187..462
                     /locus_tag="AC16V7_05500"
                     /inference="COORDINATES: protein motif:HMM:NF017239.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HIR6256609.1"
                     /translation="MVNFDRRVILPDGVLMRPVGDEAVLLNLDTNTYFALNPVGADML
                     IVLTQSSSIEQAFTRLLDMYDIAPDVMRADLQELIEDLAQQGLVLIA"
     gene            1164..1352
                     /locus_tag="AC16V7_05505"
     CDS             1164..1352
                     /locus_tag="AC16V7_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256610.1"
                     /translation="MSLAGHTVAAHLFDRQLISQHFTFCSQSATVVPVRKLIYPRKFS
                     QLPQVWQVLKNDLAGIHA"
     gene            1345..>1667
                     /locus_tag="AC16V7_05510"
     CDS             1345..>1667
                     /locus_tag="AC16V7_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256611.1"
                     /translation="MRDNKLFHNLVPSSGPERIHDLARLISGRVGAREIPAGHWADIN
                     QLAVRHGLGPLLWYILLQQDQQVCSTAVFQPLADSVNIASFNYQLLRESQSWVQAAFS
                     QAGIPS"
BASE COUNT          357 a          443 c          419 g          448 t
ORIGIN      
        1 tgttttggtg ggggcatacc cccaccaaaa caccccattt tctctcctca gtggtgggaa
       61 atcgcccaaa tgcgattgcc ctaggttttc gggcttttcc catctatttg taacctaatt
      121 atacttgagt gtttcgcatg ttcgattgtt ctaaagtgaa cgcaatcggt acgccagaaa
      181 tcatccatgg ttaactttga tcgacgcgta attcttccgg acggtgtttt gatgcggccc
      241 gtgggtgatg aggcagtgct gttaaacctg gataccaata catactttgc cttgaacccg
      301 gtgggggcgg acatgctgat cgtgcttacg caaagctcct cgatcgagca ggcattcaca
      361 cgcctcttgg acatgtatga tattgccccg gatgttatga gggcagatct tcaagaactg
      421 atcgaggatt tagcccagca agggctggtc ctgatcgcct agatggatat aactgaacat
      481 ataccaccca tttcatgtcg cctggcagga cagacgattt ggtttaatca atcgctgaat
      541 gagctacagc cattccgatt ggagggattg acctccccac cctgctctct cccggccaac
      601 ctcctggaat tagttcggga acccccgttg cttcaagcgg taggagggct agctgacaag
      661 tccacgtcca tctctctatg gcgggatgac aatggctatt tactcgaaat ccctgctgcc
      721 ggcctcttct ggacagcgat gtctggaagc tgtatctacc aggtttttcg ggtgcctagt
      781 gcgaacccat ctctcttaag cgagtctatc ttggggccgc ctctggttct ggcgttatgc
      841 ttgtctggca tctggtgctt acatgccagc gcaattgagt atgaaaacca gctcgctgta
      901 tttatagggt catctggcgc gggaaagtca acccttgcaa cctatctggc taaccattcc
      961 gggatacggc aaaccgcgga tgatattctg cctgtatccg aggcagtcca agggttgact
     1021 ttccccattt ccctcagctt aaaatacatc ctgaggagca gcccgggtta gcatttcctg
     1081 agagactgcc actttcagtt atttatctgc tggatgaggg tgtcgatgtc tcgcttgaaa
     1141 cactgagccc tgctgaggct gccatgagtc ttgctgggca tacggtggca gcccacctct
     1201 ttgaccggca gctcatcagc cagcatttca ccttctgcag ccagtctgct acagttgtac
     1261 cggttcgtaa attgatctat ccccgtaaat tttcacagct gccgcaggtc tggcaggtgc
     1321 ttaaaaatga tctggcaggc attcatgcgt gacaataaac tcttccacaa cctggtccca
     1381 agttctggtc ctgaacggat ccacgacctt gcgcgtttga tcagtggacg ggttggggcg
     1441 cgggagatac ctgcaggcca ttgggcggat atcaaccagc tggctgtccg acacggatta
     1501 ggcccgctgc tgtggtatat acttctacag caagaccagc aggtatgttc aactgctgtc
     1561 tttcagccct tggctgattc tgtaaacatc gccagcttta attaccagct gcttagagaa
     1621 agccagtctt gggtgcaggc tgccttctca caggctggta taccctc
//
LOCUS       DBNHOH010000308         2023 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_145354, whole genome shotgun sequence.
ACCESSION   DBNHOH010000308 DBNHOH010000000
VERSION     DBNHOH010000308.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_145354"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="AC16V7_05515"
     CDS             <1..897
                     /locus_tag="AC16V7_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6256612.1"
                     /translation="WTASEEESYQILSAAFEAGINFIDTADVYSRWVEGNPGGVAEAI
                     IGKWMKRNNIPRHQVIIATKVRSRIGDDPNDEGLSRNHIFRAVEASLSRLGTDYIDLY
                     QSHWYDENTPIAETMVAFDDLVRQGKVRYIGCSNYPAWRLTEALWTSKHHRIVRYDAI
                     QPHYNLVHRAEFERELADVSRAYNLGVIPYSPLAAGFLTGKYRKGKVVESSRANRARR
                     YFNDRNWSLLNQMERIGKEKGGYTISQIALAWLLNDPMITSPIIGPRSLEQLEDNLGA
                     AGLRLSQNEKKELDETSRWTDE"
     gene            complement(903..1874)
                     /locus_tag="AC16V7_05520"
     CDS             complement(903..1874)
                     /locus_tag="AC16V7_05520"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR6256613.1"
                     /translation="MAGETILIVEDNDVLRQGLHELLLSEGYAVLTASHGMDAIEQMK
                     TKPPDLILSDIAMPEMNGYLFFEEVRTKPEWVSIPFIFLTARRGRDEIFTGKKLGVED
                     YLVKPITRQELLTTIRSRLTRSQQLLLAQLEQAYEASLIMLANAIELRDHYTRGHVER
                     VRDHALAIAQQLGWTTNHLNYLAYGSILHDIGKIHVLESVLSKAGALDKMEWEEMKKH
                     PVIGAEMVKNISYLQSAIDIIRHHHERWDGTGYPDGLAGEDIPLSARIVAVADSFDAM
                     ITSRSYRVALTPEAAYDEILRSSGTHFDPSVVLAFQRAWGEIRNSLE"
     gene            complement(1847..2008)
                     /locus_tag="AC16V7_05525"
     CDS             complement(1847..2008)
                     /locus_tag="AC16V7_05525"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6256614.1"
                     /translation="MHGTGLGLAIVKEIADMHGSRIDFKSDLGKGTTFYVRFPALIGE
                     EWLVKQSLS"
BASE COUNT          537 a          521 c          472 g          493 t
ORIGIN      
        1 tggactgcga gtgaagaaga gtcttaccaa atcctcagcg cagcatttga ggctgggatc
       61 aattttattg ataccgccga tgtgtattct cgctgggtgg aaggaaatcc aggaggggtg
      121 gcagaggcca tcatcggtaa atggatgaag cgcaataata tcccccgtca ccaggtgatc
      181 atcgctacca aggtgcgtag tcggatcggc gatgatccca acgacgaagg tctctcgcgc
      241 aaccacattt tccgggcagt ggaggcctcg ttaagcaggt taggtaccga ctacatcgac
      301 ctgtaccaat cacactggta tgatgagaat acaccaattg ccgaaacaat ggttgccttc
      361 gacgatttgg ttcgccaagg gaaagtgcgt tatatcggct gctccaatta ccccgcctgg
      421 aggttgacag aggctttgtg gacatcaaaa caccatcgaa tagtccggta tgatgcaatc
      481 caacctcact acaatttggt ccaccgagcc gaattcgagc gagagttagc cgatgtcagc
      541 cgcgcctaca acctgggagt gatcccctat agccctctgg cagccggttt tctcaccggg
      601 aagtatcgca aaggtaaagt tgtcgagagc tcgcgcgcaa accgcgcccg acggtatttt
      661 aacgatcgta actggtcctt actcaaccaa atggaaagaa ttggtaaaga aaagggtggc
      721 tatactatct cccaaatagc cctggcatgg ttgctcaacg accccatgat caccagcccg
      781 ataattggac ctcgctcctt ggaacaactc gaagataacc tgggcgcggc tggattacgg
      841 ctttctcaga atgagaaaaa ggaattagat gaaactagtc gttggacaga tgaataagcg
      901 aactactcca aactattccg aatctctccc caggcacgtt gaaaggccag aaccaccgac
      961 gggtcaaaat gggtgccact ggagcgtaat atctcatcat aagccgcttc cggggtgagg
     1021 gcaacccgat aggaacgaga ggtgatcatt gcatcaaatg aatccgctac cgccacaatg
     1081 cgcgccgaaa gagggatatc ctctcctgcc agaccatcag gataccccgt tccatcccac
     1141 cgttcgtggt ggtgacggat aatgtcgatg gctgattgca aataggaaat atttttcacc
     1201 atttccgccc cgatcacggg gtgttttttc atctcctccc attccatctt atccaatgcc
     1261 ccagctttgc ttaatacgct ctccaggaca tggatttttc caatatcatg cagaatactg
     1321 ccataagcta aataattcaa atggttggtg gtccacccca gttgttgggc aatagctaag
     1381 gcatgatcgc gcacgcgctc gacatgccct cgagtgtagt gatcgcgcag ttcaatggca
     1441 ttagccagca taatcaagct cgcttcatac gcttgctcaa gctgagctaa tagcagttgc
     1501 tggctgcgcg tcaaacggga gcgaatcgta gtaagcagtt cctgtcgagt aattggtttg
     1561 accaggtaat cttctacacc aagtttcttg cctgtaaaaa tttcgtcccg accgcgccta
     1621 gcggtgagaa agataaatgg tatcgagacc cattccggtt tagtgcgaac ttcctcaaaa
     1681 aataaatatc cattcatttc cggcatggca atatccgaaa ggatcagatc tggtggcttg
     1741 gtcttcatct gctcaattgc atccatgcca tgcgatgcag tgagtacagc ataaccttct
     1801 gataaaagca gctcatgcag accctgtcga agcacatcat tgtcttctac gataaggatt
     1861 gtttcaccag ccattcttct cctatcagtg ccggaaaacg cacgtaaaat gtggtgcctt
     1921 tccctagatc gctcttaaaa tcaattcgac taccgtgcat atcagcaatt tcttttacaa
     1981 ttgccaaccc taatccagtg ccatgaattt tactttgtcg tac
//
LOCUS       DBNHOH010000309         1376 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_979650, whole genome shotgun sequence.
ACCESSION   DBNHOH010000309 DBNHOH010000000
VERSION     DBNHOH010000309.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_979650"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="AC16V7_05530"
     CDS             complement(<1..531)
                     /locus_tag="AC16V7_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256615.1"
                     /translation="MFTNTIDPDNPLTPSEVVFLNGEQFAKKVMLGNMDLLHSDEKVS
                     LTQLGQAILATAFLACEEAGAFRLEVRERKAMLGLRKVQELFAVPSNPRENLPEHSLE
                     ATLSDLATRLAPQGNSDIYTMVYAWLREDASSPWNTAMELVKAGMAKRGMLDATEEKK
                     LKIFTVTRYALPERTAK"
     gene            1037..1159
                     /locus_tag="AC16V7_05535"
     CDS             1037..1159
                     /locus_tag="AC16V7_05535"
                     /inference="COORDINATES: protein motif:HMM:NF013753.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256616.1"
                     /translation="MAGLNNKLKVLKRRCYGLFNLKHLFQRIFLDLVGYRLFAG"
BASE COUNT          302 a          377 c          370 g          327 t
ORIGIN      
        1 cttggctgtc cgctctggca gagcgtatcg ggtgacggtg aagatcttga gcttcttctc
       61 ctccgttgcg tccagcatgc cgcgcttcgc catcccggct ttcaccagct ccatggcggt
      121 gttccacggg gaagaggcgt cttcgcgcag ccaggcatag accatggtgt agatatcgct
      181 attaccctgg ggcgccaggc gggttgccag gtcagacaag gtggcttcca ggctgtgttc
      241 gggtaaattc tccctggggt tagacgggac ggcgaatagc tcctgcacct tgcgtaagcc
      301 gagcatggct ttacgctcgc gcacctccag gcggaatgcg cccgcctcct cgcaagccag
      361 gaatgcggtt gccaggatcg cctgcccaag ctgggtgagg gataccttct catcggagtg
      421 caataggtcc atattcccca gcatgacttt cttggcaaac tgctccccgt tcaagaacac
      481 cacctcgctg ggagtgaggg ggttatctgg gtcaatagtg tttgtgaaca tcgaagggtc
      541 tccttagaca agtatattac aactcttgct aacctggccc tgcaaattgc tgattatttg
      601 tttgactttt ccctgctctt cccataaact caagccatgc tggcccgcgc atattcttgc
      661 gctgtgattg ggttagaggg ggtgatcgtc gaagtcgagg tggacaccgg gcaagggctg
      721 cctgggatga ctatcgtcgg tccaaaaaac cgtgcaattc agcctctccc tggcagacgt
      781 gccgtatgca aaggtcatcc aggcgacgca tgatcaattg tagaggcatg cataattcta
      841 acgtgaattc cagcaacaac tgacaaaagt ttttgatcaa cacatctccc cggcagatgc
      901 caaaaccaaa atccgggtct ggatcacacg cctcaccaga agcgggttgc gctgtttcga
      961 taagttcatc aacaccctgg gccgctggtg acaggaaatc atcaactact ttgcaaaccg
     1021 ggaaagcagc ggctttgtgg caggactgaa caacaaactc aaggtgctca agcggcgttg
     1081 ctatggattg ttcaacctga agcatctttt ccaacgcatc ttcctcgatc tagtaggcta
     1141 tcgcctgttc gcaggctgac caccatatgt ggccgaatca cagtaactcc cctcagggca
     1201 atcgcatgta ccctgagggg catgcgcgat cccgcataaa tagtgtttgg ggtgttttgg
     1261 tgggggtatg tccccaccaa aacacccgat tttctctcct cagtggtggg aaatcgccca
     1321 aatgcgattg ccccgtaact cccagtgggg cgtaattcag ttttggggga aaacta
//
LOCUS       DBNHOH010000310         2827 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1555799, whole genome shotgun sequence.
ACCESSION   DBNHOH010000310 DBNHOH010000000
VERSION     DBNHOH010000310.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1555799"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="AC16V7_05540"
     CDS             <1..473
                     /locus_tag="AC16V7_05540"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR6256617.1"
                     /translation="VSSPFPGNCIVPDGCRLRYDRRLLPGDTRQSLMDSLHEAMANHQ
                     RWSIGFQPVRLETYTGHVLEGEHFYPAWTLPEDSPWLAKARRGLEMNGLTAKSMAVPY
                     CTNASFTAGQAGIPSLIFGPSDISLAHIVDEYVEVDELMRAVAGFTGLAEALAE"
     gene            complement(545..1012)
                     /locus_tag="AC16V7_05545"
     CDS             complement(545..1012)
                     /locus_tag="AC16V7_05545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6256618.1"
                     /translation="MKSWHDFSIEEPELAQIGQALLFRSRIGLAFLATLRKDGAPRLH
                     PVSLIISNGHLYVLIPASSPKCADLVRDGRYAMQAFPSQGEENEEFYLAGRAERIQDT
                     MIRQAVIKHTNILAGEDEVMFEILLDRAMYTKLVDYGTPDERPVHRKWRATSS"
     gene            complement(1084..1950)
                     /locus_tag="AC16V7_05550"
     CDS             complement(1084..1950)
                     /locus_tag="AC16V7_05550"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6256619.1"
                     /translation="MLNSITPHVYWLPPDAATDRPILGAIVGEQRTLIVDAGNSPAHA
                     NLLLGELSKLHIAFPDYLVLTHWHWDHVFGSSAFKLPTFASKETRRIVAEMARLNWSD
                     AALDRRVEDGLEISFCRDMIKAEMPDRSNLTITPPDIAFDSPIQLDLGGLTCYIVPVG
                     GDHSSDASIIYVEEDKIMFLGDCLYTDIYSGPPRFTTRKLFPLLDRLLSFDTDYYLAA
                     HESNPMSRTDMVEYAGLLKTIGCVVEKIGPDRQSILAALQTTFSQQLNDEHVEIADAF
                     LAGLSKSTPPAE"
     gene            complement(2161..2586)
                     /locus_tag="AC16V7_05555"
     CDS             complement(2161..2586)
                     /locus_tag="AC16V7_05555"
                     /inference="COORDINATES: protein motif:HMM:NF013003.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HIR6256620.1"
                     /translation="MAEHEHLIQWQEGFEAWNKWRAENQDTRPNLLGADLSWASLLWA
                     DLNDTDMRRVNLNGANLSGARLIGADLRFANLTMTDLRGANMGGSNLSGANLTGANLT
                     GVNLHGADLSETRLTGARYNSATRWPIGFDVNASGAILG"
BASE COUNT          677 a          763 c          708 g          679 t
ORIGIN      
        1 tcgtctctag cccattccct ggcaactgca tcgtccccga tggctgccgc ctgcgttacg
       61 accggcgcct tttgcctgga gacacccgcc aaagcctgat ggatagcctg cacgaagcaa
      121 tggcgaacca ccaacgttgg agcatcggct tccagcccgt ccgcctggaa acctacactg
      181 gtcatgtact ggaaggtgag catttctatc cggcttggac gctgccagaa gattcccctt
      241 ggctagcgaa agcccgccgg ggattggaga tgaacggttt aactgcgaaa agcatggcgg
      301 tgccctactg tacaaacgct agcttcactg ctgggcaagc gggcatcccc agcttgatct
      361 ttggtccatc cgatatcagc ctggcacaca tcgtcgacga gtacgtcgaa gttgacgagc
      421 tgatgcgcgc cgtagctggc tttaccggtt tggcagaggc actggcggag taatctccag
      481 gcatgacctg tcagttactc caacttatag aatatctagt ctagatctca tgtgaccaag
      541 aaggttatga acttgttgcc cgccacttcc tgtgaacagg tcgttcgtca ggggtgccat
      601 aatcgacaag cttggtatac atggcgcggt caagcagaat ctcaaacatc acttcatctt
      661 ctcctgccag aatgttcgta tgtttgataa ctgcctggcg gatcatcgta tcttgaattc
      721 gctcagcgcg accggcgagg taaaattcct cattttcctc accctgcgaa ggaaacgcct
      781 gcattgcata tctgccatcg cgcactaggt cggcgcactt cggagagctt gcagggatga
      841 gcacgtatag atgaccgttg gagatgatca ggctgacagg gtgcaatcga ggggcgccat
      901 ccttgcggag agttgccaaa aaagcaagcc caatgcgaga acgaaaaaga agagcctggc
      961 caatttgagc caattctggc tcctcgatag agaagtcgtg ccaagatttc atgtaatgat
     1021 catcctctat tgctgccgaa acgcgagcgt ataagtatcc cccgttgtgc gcttcagcca
     1081 tcctcactct gctgggggcg tggatttgct caagcccgcc aagaaggcat cggcgatttc
     1141 aacatgctcg tcatttaatt gctgactgaa tgtggtctgt aaagcggcaa gtattgactg
     1201 acgatctggg ccaatttttt ctactacaca accaatggtc ttgagtagac ccgcatattc
     1261 gaccatatcc gttctggaca tagggttaga ctcatgcgct gcgagatagt aatccgtgtc
     1321 aaaacttagc aggcggtcga ggagcggaaa tagtttacgc gtagtgaatc tgggcggacc
     1381 gctgtaaata tcggtgtata aacaatcacc cagaaacatg attttgtctt cttccacata
     1441 gatgatactg gcatctgatg agtgatcgcc tcccacggga actatgtagc aagtgaggcc
     1501 tccaagatca agctggatgg gagaatcaaa ggctatatcg ggaggcgtaa tggtgagatt
     1561 gctccggtcg ggcatctcag ccttgatcat atcccggcaa aatgatatct cgagcccgtc
     1621 ttcgacgcgt ctatccagcg ccgcatcact ccagtttaaa cgtgccattt cggcgacaat
     1681 acgcctggtt tcttttgaag cgaaggtggg taatttgaat gctgatgatc caaatacatg
     1741 atcccaatgc cagtgagtga gcactaggta atcgggaaat gcaatatgga gcttcgacaa
     1801 ttcacccaat aacaggttgg cgtgtgctgg agaattccct gcatcaacaa tcaatgtgcg
     1861 ttgctcgccc acaatggcac ccaggattgg gcgatctgtt gcggcatcag gtggtaacca
     1921 atacacatgg ggcgtgattg aattcaacat ggaatgagct ttatcaattt gtatccgctt
     1981 ttattgagca tcaccatggc tccagggaga ttagaggctc tcacatactc attttggccc
     2041 aatttcccaa tttgttacag gatcggcttg ctgattcaat gtctaacgcc atcccctctt
     2101 ccctctcgtc tcgttcccct gcaaggactg atcgaaaaag agagagccat caaccactgg
     2161 tcagcccagt atagcccctg aagcattcac atcaaagcca atgggccagc gggtggcgct
     2221 gttgtagcgc gccccggtca gtctggtctc gctcaggtca gcgccgtgca ggtttacccc
     2281 ggtcaggttg gccccggtca agttggcgcc gctcaagtta gacccaccca tattggcacc
     2341 gcgcagatcg gtcatggtca ggttggcaaa cctcaggtca gcaccgatca agcgcgctcc
     2401 gctcaagtta gccccattca agttcacccg gcgcatatcg gtatcgttta gatcagccca
     2461 caacaggctt gcccagctca ggtccgctcc aagcaggttt ggacgggtat cttgattttc
     2521 agctcgccac ttattccagg cttcaaagcc ctcttgccac tgtattagat gttcatgttc
     2581 tgccaattgc aacactcctg gtacattggg tcgataattc aattaagaat cagaaagcac
     2641 accgcagggt ctaccattat aaaatcaagt caccaacccc tgctgacatc gcaggcacac
     2701 cggttgaagc atgatgattg tcctctcagc agagtatagc cccggaattt tccacctcaa
     2761 agccatccgg ccagcgggtg aggctgtcgt agatcgcccc actcaaattt gccccactca
     2821 ggtttgc
//
LOCUS       DBNHOH010000311         6152 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1555924, whole genome shotgun sequence.
ACCESSION   DBNHOH010000311 DBNHOH010000000
VERSION     DBNHOH010000311.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6152)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6152)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6152
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1555924"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..787
                     /locus_tag="AC16V7_05560"
     CDS             5..787
                     /locus_tag="AC16V7_05560"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0030497 - fatty acid elongation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HIR6256621.1"
                     /translation="MKLLEGKTALVFGLANERSIAWGITKAFHEHGATLGISYAGEVL
                     ERRVRPLAAEIGCNFVESCDVTADEQIEMVAQKAAEAFGQIDILVHAIAFANRDELSG
                     PYYNTSRAGFHTAMDISVYSFTALAKAFQPLLRPGGALLTLTYYGAEKVSPHYNVMGV
                     AKAALEASVRYLAYDFGKQKVRVNAISAGPIRTLAAAGVSGFKSMYRQFAEIAPLHEN
                     ITIEDVGGAAVFLCSDLAGKTTGEVTYVDSGYNILGIPEPVD"
     gene            790..1233
                     /locus_tag="AC16V7_05565"
     CDS             790..1233
                     /locus_tag="AC16V7_05565"
                     /inference="COORDINATES: protein motif:HMM:NF016408.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactofilin family protein"
                     /protein_id="HIR6256622.1"
                     /translation="MFRRREDPLLPPTQPMPTERVTSVLGSGIVWKGNLSGVGGIRIE
                     GAFEGDISLRGMLVIGETGRVTCEHLRANVVIVAGAVQGNITAEKVEIRRTGRVWGDV
                     TTAAFATEEGAFLRGQIRMEEQVELGTGASSEADAEAESEAGVDG"
     gene            complement(1239..2057)
                     /locus_tag="AC16V7_05570"
     CDS             complement(1239..2057)
                     /locus_tag="AC16V7_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256623.1"
                     /translation="MRRSNRELWLSFAAIVCISLAYLFVLAWQDGIPRASEFFGHSLG
                     ILGFIMMVMTETLYTLRKRSHSARWGRMADWLDFHIFTGIVGPYMVLLHSSWKFNGLA
                     GIVMLLTVTIVASGFIGRYIYTSVPRTVDGVEVEARLLEEQIAAADQELKNWLSAPDK
                     SLRNLAHNFSTAPLGSAGQFTLIVGRGLLDLTYQLNWWLKRRRLSSQERQQARVLEKL
                     LSRRRTLEIQLASLTTARRLLALWHAVHIPIGMVLFTAAIIHIIAAIYYATLLR"
     gene            complement(2044..3366)
                     /locus_tag="AC16V7_05575"
     CDS             complement(2044..3366)
                     /locus_tag="AC16V7_05575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6256624.1"
                     /translation="MRRYVMIGSGVASIAAIEAIRSVDPRGEITLIGDESHGYYSRPG
                     LAYYLNGEINERQLFPMDQANYHKLNIRRLHARVERIYPEQHLVRLHNGATMPYDRLL
                     IAVGAEANMMNIPGVDLHGVVKLDKLDDARWIIKLARKARTAVVVGGGITALEIVEGL
                     VKHGVRTHYFLRGDRYWSNVLDKTESQIVENRLRGTGVHIHYHTELREILGKNGRVVG
                     VRTQDNQTMRCDILAIAIGIRPRKELAEISGLCVDRGVEVSETLQSSAPDVFAAGDVA
                     QVYDPFTGKSVVDSLWGPARDQGHTAGLNMAGITTPYYKLIAFNVTRLADLTTTIVGT
                     VGRGDDQDVIGIVRGDSETWRQLPDAIAVQSHFEINRLRLQIGKNNLLGAIIMGDQTL
                     SRPLHQLITEQADITPIRQQLLQPGAPLVDILADFWCHWKARYAFATQ"
     gene            complement(3368..3649)
                     /locus_tag="AC16V7_05580"
     CDS             complement(3368..3649)
                     /locus_tag="AC16V7_05580"
                     /inference="COORDINATES: protein motif:HMM:NF024634.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR6256625.1"
                     /translation="MNITSLFKLEITRRKALVQGYVVPEPARCVQCGVCTYNCPINID
                     VRQHAWKGIPVKDSHCLTCGECVARCPRGVLRFEGTSMFTSQTPGLAGD"
     gene            complement(3646..5442)
                     /locus_tag="AC16V7_05585"
     CDS             complement(3646..5442)
                     /locus_tag="AC16V7_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256626.1"
                     /translation="MRKFLLFSIPIALALAMLVSSMGAYLAYAAPFQPGSSLFPLQEF
                     AEEKGAFLFINPTGKANYYLDLSERRVMDLLELARTDREVIALKALDNALDQAEIAIA
                     AAPDEDLPALMARLANVVTQIEKLVSVLTIAPIEDPDTVAALLAQVETLRSLLAGSGA
                     STAQSLENARKLLVLSVVGTHEDPSTLDVSAIAAIDPRIINFPAGSLGAQHTFFPLGG
                     GHAGLACESCHKNGQFSGTSSQCIDCHRNVVPANHYPGDCASCHTILAWDDIHFNHQV
                     VATKDCQSCHARPKNHFSGQCSACHSTQAWTPARFNHEATGAKDCQSCHTRPKNHFSG
                     QCSNCHSTSAWRPAKFDHTGLTDCQSCHKRPSNHFSGQCSACHNSSAWRPAKFDHTGL
                     TDCQSCHSRPRNHFSGQCSACHTTSAWKPANFNHTGLTDCQSCHNRPSNHYSGQCSNC
                     HNTSGWGSASFSHTGLTDCQSCHNRPSNHYSGQCSNCHNTNGWGSVSFSHSGLTDCQS
                     CHNRPSNHYSGQCSDCHNTQNWSDVRLSNHTFPINHKGANGRCSVCHPSGGSSYTCYN
                     CHDRNKTEEHHNEKGITDIASRCADCHPRGEE"
     gene            complement(5458..>6152)
                     /locus_tag="AC16V7_05590"
     CDS             complement(5458..>6152)
                     /locus_tag="AC16V7_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR6256627.1"
                     /translation="CHTPDAWSPATWEEAPFEHAIQAGFSLSLHRVDYEGNPLSCLGC
                     HSGDAQNFEQESCISCHSQGDERAAFMVEHQAQYGSDCVACHDGVDRMANFNHEAVFP
                     LDGRHAEIACQECHADQVFTGTPATCAECHAEPDIHAGFFGLECQYCHSAQAWTPALL
                     IQHRFPLDHGEQGEITCNACHPDSYIEYTCYGCHDHQPDETAAKHQEEGISLEKLADC
                     ASCHPAGLKEDN"
BASE COUNT         1312 a         1583 c         1835 g         1422 t
ORIGIN      
        1 atagatgaaa ttacttgaag gtaaaaccgc gctggtattt ggtctagcca atgaacgctc
       61 gatcgcttgg gggattacca aggcttttca tgagcatggc gccaccttgg gcatcagcta
      121 cgcaggcgaa gtcttggagc ggcgtgtccg cccccttgca gcggagatcg gttgtaactt
      181 tgtagaatcc tgtgacgtga ctgccgacga gcagatcgag atggtggcac aaaaggctgc
      241 ggaagccttt gggcagatcg atatcctggt gcatgccatc gcatttgcaa accgggatga
      301 gctgagcggg ccgtattaca ataccagccg ggctggtttt cacacggcga tggatatcag
      361 cgtgtattcc ttcaccgcgc tggctaaagc cttccagccc ttattgcgcc ccggtggcgc
      421 cctgcttacg ctgacatatt atggggcgga gaaagtttcc cctcactata atgtgatggg
      481 tgtcgccaag gcggctttgg aagcctcggt gcgctacctg gcgtatgact ttggcaagca
      541 gaaggtgcgt gtaaacgcga tctccgcagg cccaatccgc acactggcgg cagctggcgt
      601 atctgggttt aagagcatgt accgccagtt tgctgagatc gctcctctac atgaaaatat
      661 caccattgaa gatgtgggcg gtgcggcggt attcctgtgc tccgacctgg caggtaagac
      721 cacgggcgag gtaacgtatg tcgattcggg gtataacatt ctgggcatcc cggagccggt
      781 tgattaaaga tgtttcgtag aagggaggat cctttacttc cccccaccca acccatgcct
      841 acggagaggg ttacctcggt gttgggcagc ggcattgtgt ggaaaggcaa cctgagcggt
      901 gtgggtggga tccgaattga aggcgccttc gaaggcgata tttccctgcg gggtatgttg
      961 gtcatagggg agaccgggcg ggtcacctgc gagcacctgc gcgctaatgt ggtcattgtg
     1021 gctggagcag tgcagggtaa catcactgcc gaaaaagtgg agatccgtag gactggacgt
     1081 gtatgggggg atgtaactac tgcggcattt gccactgaag agggggcatt tctacgcggt
     1141 cagatccgca tggaagagca ggtagaattg gggactgggg caagctcgga agcagatgca
     1201 gaagctgaat ccgaagccgg ggttgacgga tagatcgatt atcgcaagag cgtggcgtaa
     1261 taaattgctg cgatgatgtg gatgatggca gcggtgaaga gtaccatgcc aatggggata
     1321 tgcaccgcgt gccacagcgc cagcagacga cgtgccgtgg ttagtgatgc cagctggatc
     1381 tccagtgttc tccttcgaga tagcaatttc tccagaaccc gggcttgctg gcgttcctgc
     1441 gaggacaagc gccggcgctt tagccaccaa tttaattgat aggtcaaatc taataagcct
     1501 ctgcccacaa ttaaggtgaa ctgccctgca gaacccagtg gcgcggtgga gaaattatgt
     1561 gccaggttgc gcagagactt atctggcgcg ctgagccagt tttttaactc ctggtctgcg
     1621 gcagcgattt gttcttcgag caggcgcgct tctacctcca cgccgtcaac ggtacgcggc
     1681 acggaggtat agatatagcg accaataaag ccgctggcga cgattgtgac agtgagcagc
     1741 atcacgatcc cagccaggcc attaaacttc catgaggagt gcaacagcac catatatgga
     1801 ccgacaatcc cggtgaagat gtggaagtcc agccagtctg ccatgcgccc ccagcgtgcg
     1861 ctatggctgc gctttcgtaa agtgtagagc gtctcggtca ttaccatcat aataaaaccc
     1921 aggatgccga ggctgtgacc aaagaattcg ctggcgcgcg ggatgccatc ctgccaggcc
     1981 agaacgaaca agtacgccag gctgatgcat acgatggcag caaatgagag ccacagctcg
     2041 cggttactgc gtcgcaaagg cgtatctcgc tttccaatgg caccagaaat cagccaggat
     2101 atccaccagc ggcgcacccg gctggagaag ctgctggcgg atcggcgtga tatccgcctg
     2161 ttcggtgatc aattggtgta atggtctgga gagagtctgg tcgcccataa tgatcgcacc
     2221 caataagttg tttttaccta tctggaggcg caggcggttg atctcgaagt gggattggac
     2281 ggcgatggca tcgggaagct ggcgccaggt ctcgctatcg ccgcggacga tgccgatcac
     2341 gtcctgatcg tcgcctcgac cgactgtgcc gacgatggtt gtggtcaaat cggccagtcg
     2401 ggtcacgtta aatgcaatta gcttatagta tggagtggtg atacctgcca tgtttaatcc
     2461 ggcagtgtgt ccttggtctc gcgctggtcc ccaaaggctg tccactaccg acttgccagt
     2521 gaaggggtca tacacctggg ctacatcacc cgctgcgaac acgtcgggag cactgctttg
     2581 caaggtctcg ctcacctcga cgccgcgatc gacgcacaag ccagatatct ccgccagctc
     2641 tttgcgagga cggataccaa ttgcaatagc tagtatgtca caccgcatcg tctggttgtc
     2701 ttgggtgcgc acgcccacca cacgcccgtt tttgcctagg atctctctga gttcggtgtg
     2761 gtagtggata tgcactccag ttcctcgcag acggttttct acaatttgtg attcggtttt
     2821 atccaacaca ttgctccagt aacgatcgcc gcgcaagaag taatgtgttc tcacaccatg
     2881 ttttaccagc ccttcgacaa tctccaatgc ggtgatgccc ccgcccacaa cgaccgccgt
     2941 tcgcgctttg cgagccagct tgatgatcca gcgggcgtcg tccagcttgt ctagtttgac
     3001 cacgccgtgt aagtccacgc cagggatatt catcatgttt gcctcggctc ccacagcgat
     3061 caacaggcgg tcgtagggca ttgtggctcc attgtgtagg cggacgagat gttgctctgg
     3121 ataaattcgt tctacccgtg cgtgcagacg gcggatattt agcttatgat agttggcttg
     3181 atccatagga aaaagttgcc gctcattgat ctcaccgttc aggtaatagg ctaaacctgg
     3241 acgcgaatag tacccgtgag attcatcccc aatcaaggtg atctctccac ggggatcgac
     3301 gctgcggatg gcttcaatcg ctgcgatgga agccacaccg ctgccgatca tcacgtagcg
     3361 gcgcatctca gtctccagcc aatccgggcg tctgtgatgt gaacatgctg gtaccctcga
     3421 agcgaagcac accgcgcgga cagcgggcaa cacattcgcc gcacgtcagg cagtggctgt
     3481 ccttcaccgg gatccctttc caggcatgct ggcgcacatc aatgttgatt gggcagttat
     3541 atgtacaaac accgcactgc acacagcggg ccggttcggg aactacgtat ccctggacca
     3601 gggccttgcg ccgggtgatt tcgagtttaa atagactggt gatgttcatt cctcacctcg
     3661 aggatggcaa tctgcgcaac gggaagcgat atcggtgatg cctttttcat tgtgatgttc
     3721 ttccgttttg ttccggtcgt ggcagttgta gcatgtatat gaacttccgc ctgaagggtg
     3781 acagaccgaa cagcgaccat ttgcaccttt atgattaatg gggaaggtgt gattactcag
     3841 gcgcacatcg ctccagtttt gggtgttatg gcagtcggag cactgcccgg agtaatggtt
     3901 ggacgggcga ttgtggcagg actggcaatc ggtcaagccg gagtgactga aactcaccga
     3961 tccccatcca ttggtgttgt ggcagtttga gcactgcccg gagtaatggt tggatggtcg
     4021 gttgtggcag gactggcagt cagtcagacc agtatggctg aaactcgctg atccccatcc
     4081 actggtgttg tggcagtttg agcactgccc ggagtagtgg ttggatgggc ggttatggca
     4141 agactggcaa tcggtcaggc cagtatggtt gaagttagct ggcttccagg cgctggtagt
     4201 gtggcatgcc gagcactgtc cgctgaagtg gtttctcggc cttgaatggc aagactggca
     4261 atctgtcaga cctgtgtggt caaatttggc cggtctccat gcgctggaat tgtggcatgc
     4321 cgagcactgc ccgctgaagt ggttcgacgg ccttttgtga caagactggc agtcggtcag
     4381 tccggtgtgg tcaaatttgg ctggtctcca tgcgctggtg ctgtgacaat tggagcactg
     4441 cccgctaaag tggtttttcg gccttgtatg gcaagactgg caatcttttg cgcctgtggc
     4501 ctcatggtta aaacgcgccg gtgtccatgc ctgggtgcta tggcaggcag agcactgccc
     4561 gctgaagtgg ttttttggcc gagcatggca ggactggcag tccttggttg ccactacctg
     4621 gtgattgaag tggatatcgt cccatgccag gattgtatga caggaagcac aatcgccagg
     4681 gtaatggttg gcaggcacca cattgcgatg gcaatcgata cattggctgg aagtaccgga
     4741 gaattgcccg tttttatggc agctttcaca cgctaaacca gcgtgccctc cacctagggg
     4801 gaagaaggtg tgttgcgccc cgaggctgcc ggctgggaag ttgatgattc ttgggtcaat
     4861 cgcggcgata gcggagacat ccaatgtgga tgggtcttca tgcgtgccaa ccacagaaag
     4921 aacgagcagc ttcctggcgt tctccagtga ttgagcggta gacgccccag atccagccag
     4981 cagcgagcgc aacgtctcca cctgggctaa aagcgctgcc acagtatcag ggtcttcaat
     5041 tggggcgatg gttagcacgg agacgagctt ttcgatctgt gtcaccacat tcgccaggcg
     5101 cgccatcaac gctgggagat cttcgtcggg cgccgcggcg atagctattt ctgcctggtc
     5161 cagggcatta tccagggcct tcagtgcgat aacctcccga tctgtgcgtg ctaactcgag
     5221 aagatccatt actcgccgct cactcaggtc taagtagtaa ttcgccttgc cggtagggtt
     5281 gataaatagg aaggcgcctt tttcctcggc gaactcctgc agtggaaaaa gggaactgcc
     5341 aggttggaag ggagcggcat aagccaggta cgctcccata ctcgacacaa gcatagctag
     5401 cgcaagtgca atgggtatcg agaacagcag gaattttcgc atggcgtttt cctcaggcta
     5461 attatcctct ttgagaccag ctgggtggca actggcgcag tccgcaagct tttcgagcga
     5521 gattccttct tcctggtgct ttgcagcagt ttcatctggc tggtggtcat ggcacccgta
     5581 gcaggtgtat tcgatataac tgtctgggtg gcaggcattg caggtaattt caccttgttc
     5641 gccgtgatcc aagggaaagc ggtgttgtat caacagggca ggtgtccagg cttgggcgct
     5701 gtggcaatac tggcattcca gcccaaagaa cccggcatgg atgtctggct cggcgtgaca
     5761 ctctgcacaa gtggccggcg tgccggtgaa gacctgatca gcatgacatt cctggcaggc
     5821 gatctcggcg tggcgcccgt ccagagggaa gacggcctcg tggttgaaat tggccattcg
     5881 atccacgccg tcatgacagg cgacacagtc gctaccatat tgcgcctggt gctcgaccat
     5941 gaaagcagcc ctttcatccc cttgagaatg gcaggatata cagctttcct gttcaaagtt
     6001 ctgggcatct cctgagtggc aaccaaggca ggagagtgga ttgccctcat aatccacacg
     6061 gtgtaaggat aggctgaagc ccgcctggat ggcgtgctcg aagggtgcct cttcccaagt
     6121 ggcaggagac caggcatcgg gggtatggca gg
//
LOCUS       DBNHOH010000312         4132 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_467041, whole genome shotgun sequence.
ACCESSION   DBNHOH010000312 DBNHOH010000000
VERSION     DBNHOH010000312.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_467041"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="AC16V7_05595"
     CDS             complement(<1..844)
                     /locus_tag="AC16V7_05595"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR6256628.1"
                     /translation="MFDSISPNPDGPVLVIGAAGVDIVGRLRGELRSKTSTPAVIRSS
                     YGGVARNVAENLVRLGQPVNLISVVGEDDEGDRLLQGLIAAGVNPERVLRTSQYVTNS
                     YLAIIDSQGELQFALDDMRAINCLTPQFIREQAALFKEASILFVDANLPKDTLRTVMS
                     LARRAHLPICADPTSVSLAVRLRPYLSRFFLMIPNNAEAAVLSERTSPVGGRRQALEV
                     AKYLISQGVDIVIITMAEQGLCYATSESSGYIPAIKTSILDPTGAGDALTATVIFALL
                     NDIPL"
     gene            complement(849..2603)
                     /locus_tag="AC16V7_05600"
     CDS             complement(849..2603)
                     /locus_tag="AC16V7_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256629.1"
                     /translation="MKYELSLNAMITDWLRWLAPLKDPVFGALDLLTAWFFDFGSGWA
                     ILRWFAITLVGAGLWVLLAIQPLEKLAGAIDPNLVFDQVFYVICQVRETIVALPPEEA
                     AVHFCGAYANPEAISFEPLIFEIPLQWLFGAGVFRHILLLAFACWLAFKIASQYQADV
                     YDIPDISCSEHFLMQAALINPRNVINIQNASQRPLEEHLPLYLIGGPGKVCVHLENAA
                     LFEKMDGVPRVVGPTVRQPVTLDGFERLRKVIDLRDQKESFDIRGRSQDGIRVAAKDV
                     QVVYSVFRNHIEPSYEQPYPFQDPRAIENLIFQQSQQVWNLSVGIQIRGALLDFFAQH
                     TLNEFLAMIQEPELKKAQEYELELMKESRRLSGSEEPIGTPPIENVDGIEYVSRPQIT
                     DLFYERAEETKLARGAEIDWVGVGTWDFPAQLIPVRHQEAWRITHDNLMKNNPAVFNG
                     LRWQYKINETLRLIQMVPIATFKKMISTPDIHTDDAIRALILAYHTQMREAYADYERS
                     ERELEKLESQETDPVKVLEHREKLGKLIAEKQRISEVMAFLARFIGRWLVGPSLTGPT
                     PKGPLPTGPQAAESGNGG"
     gene            complement(2639..3970)
                     /locus_tag="AC16V7_05605"
     CDS             complement(2639..3970)
                     /locus_tag="AC16V7_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256630.1"
                     /translation="MVVAAFLFWIAFFAQFSLPVRNFGQRILASNYLFSYAIGRHGPA
                     IKIDNGDIPDRYPRDKPHRRGVIVLDTASAAVLRTRTQFTRSVGPGLVFTLPGEYLAG
                     SVDLHQKISHVPALGPLADEMYPFAPWDQENEAEEDHEERQKRRFETSGETRDAVEVV
                     PNIHTVTRIDPDHRPNQLSGVPADRGLYSWLREYMQSDSHTRFGYNPEAVRLIVTGEA
                     VDPMVNMPIPEKRQIPWYQFPAYLAVDLWREYLRRFTFEELFSRLEDYGGQTAFQVIQ
                     DKIFERLTRPYVLEINQVGKPTGRLVESREFAMLRERGIRVLAAPIRNLRFEKKIDQQ
                     LEDQWVSFWRLRAEAERDYINRRRSYSIHEGEKDAQREFAFWATRRIESSLLQSPKPP
                     SQAEARIQMRVVLERLLRGTLGQCLHDTRLHQRLSGEESQLITIIEWLRRL"
BASE COUNT         1052 a         1127 c         1012 g          941 t
ORIGIN      
        1 caagtgggat gtcgtttaac aatgcaaaaa tcacagtcgc cgtgagtgca tcaccagcac
       61 ccgttggatc taaaatggat gttttgatgg ctgggatata accagaactc tccgaagtcg
      121 cataacataa cccttgctct gccatggtga tgataacgat atctacaccc tgtgatatga
      181 gatacttagc cacctctaac gcctggcgac gtccaccgac tggcgaagtt cgctcggata
      241 atactgcggc ttcagcgtta tttgggatca tcaaaaaaaa tcgcgataag taagggcgca
      301 agcggaccgc cagtgaaacc gaggtaggat cggcgcatat aggcagatgc gcccggcgcg
      361 ccagcgacat cacggtgcgg agcgtatcct tgggcaaatt ggcatcgaca aagagaatgg
      421 aggcttcttt gaacagcgca gcctgctcac ggataaactg gggcgtaagg cagttgatgg
      481 cgcgcatatc atcaagcgca aattgcagct ccccttgact atctattatc gccagataag
      541 agtttgtcac atattgggaa gtgcgcaaaa ctcgctccgg attcactccg gcagcaatca
      601 agccttgcaa caggcgatcc ccctcatcat cctctcctac cacgctaatc aggtttacag
      661 gttgccctaa gcgaaccaga ttctctgcaa cattgcgggc taccccgcca taggaggagc
      721 gaatcaccgc cggtgtcgaa gtctttgatc gcagctctcc ccgcagcctg ccaacaatat
      781 ccactcctgc cgcgccaatc accagtactg gaccatctgg atttggggaa atgctatcaa
      841 acatcgcctt agcctccgtt gccagactca gccgcctggg gaccagtcgg taatggacct
      901 ttcggtgttg gtccagttag agacggtcct accagccagc gaccaataaa gcgtgcaagg
      961 aaagccatca cctcggaaat cctctgcttt tctgcgatca atttcccgag tttctcgcgg
     1021 tgctccagca ctttcaccgg gtcagtctcc tggctttcaa gcttttcgag ctcacgttcc
     1081 gaacgctcat aatcggcata tgcttctcgc atctgggtat gataagctaa aatcagagca
     1141 cgaattgcgt catcggtgtg gatatcgggg gtgctgatca tttttttgaa agttgcaatg
     1201 ggcaccatct gaatcagccg cagggtctcg ttgattttat actgccaacg caaaccatta
     1261 aataccgctg gattattttt catcaggttg tcatgggtaa tgcgccaagc ttcctgatgg
     1321 cgcacgggga tgagctgggc tggaaaatcc caagttccaa ctccaaccca atcgatttca
     1381 gcaccgcgcg ccagcttggt ctcctcagcc cgctcataga acaggtcggt gatttgcggg
     1441 cgtgaaacgt attcaatacc atccacattt tctatgggag gcgtgccaat tggctcttct
     1501 gaaccggata gtctccggct ttctttcatg agctccagtt catattcttg ggctttcttt
     1561 aattccggtt cttgaatcat cgccaagaac tcattcaagg tgtgttgggc aaagaaatcc
     1621 agcaatgcac cgcggatctg gatgcccaca ctcagattcc atacttgctg gctttgttgg
     1681 aagatgaggt tttctatagc tcttggatct tgaaaaggat agggttgttc atacgaaggt
     1741 tcaatatgat ttcgaaaaac gctgtagact acctgcacat ctttggcagc cacgcggatg
     1801 ccatcctgcg accgcccgcg aatatcaaaa gattctttct gatcacgcaa gtcaatcact
     1861 ttgcgcaaac gctcgaaacc gtccagggtg acaggttgtc tcaccgttgg gccaacaacc
     1921 cttggcaccc catccatctt ttcgaaaagc gcggcatttt ccaggtgcac acatactttt
     1981 ccaggaccac cgatcaggta caatggcaaa tgctcctcca gtggtcgctg gctggcattc
     2041 tggatgttga tgacattacg gggattgatt aatgccgcct gcattaaaaa atgctcggag
     2101 caggagatgt ctggtatatc gtagacatct gcttgatact gactggcgat cttaaatgcc
     2161 agccagcagg caaacgccag cagcaagata tgacggaata cgcccgcacc gaacaaccat
     2221 tgaagtggga tttcaaatat cagcggctcg aatgagatcg cctctgggtt tgcgtacgca
     2281 ccacagaaat gaacagcagc ttcttcaggt ggaagagcaa caatcgtctc ccgcacttgg
     2341 caaatcacat aaaacacctg atcaaaaacc agattggggt caatggcacc agcaagcttc
     2401 tccagcggtt ggattgccaa aagcacccat aaccctgcgc ctaccagggt aattgcgaac
     2461 cagcggagta tcgcccagcc agaaccgaag tcgaaaaacc aggcagtcag cagatcaagg
     2521 gcgccgaaga caggatcttt aagaggagcc agccagcgaa gccagtctgt gatcatcgcg
     2581 ttcaggctta gttcgtattt catagtcctg ggcttacggt ttcggttcat tagccagcct
     2641 acaacctgcg caaccattcg atgatggtaa tcaactgcga ctcttctccg gataaacgtt
     2701 gatgcaaccg ggtatcatgc aaacactgac ctagagtacc ccgcaacaaa cgctccaata
     2761 ccacgcgcat ctggatgcgg gcttccgctt gcgagggtgg ctttggagat tgcagcaagc
     2821 tgctctcaat acggcgagta gcccaaaatg caaactcgcg ctgggcatcc ttctcacctt
     2881 cgtggatgct atagctcctc cgccggttaa tgtaatcccg ttcggcttcc gcgcgcaggc
     2941 gccaaaacga cacccattgg tcctccagtt gttgatcgat ctttttttca aagcgcaggt
     3001 tacgaattgg agcagctagc acccggattc cacgttctcg caacatggca aactcacggc
     3061 tctcgaccag tcttcccgtt ggtttaccca cttggttaat ctctagcaca taagggcgtg
     3121 tcaagcgctc aaagatctta tcctggatga cctggaaagc agtctgtcca ccataatctt
     3181 ccagcctgga aaagagctct tcaaaggtaa agcgacgcaa gtattcgcgc cagagatcca
     3241 ccgccaaata agccgggaat tggtaccagg ggatttgtcg cttttcgggt attggcatat
     3301 tgaccatcgg atcaactgcc tcaccagtga cgattaaacg caccgcttca ggattgtatc
     3361 caaaacgagt gtgcgaatcg ctctgcatgt actcacgcag ccaactgtac aacccgcggt
     3421 ctgccgggac acccgagagc tggttaggcc gatggtctgg atcgatgcgc gtaactgtat
     3481 ggatattcgg tacaacttcc actgcatcac gtgtctcccc gctggtttcg aaacggcgtt
     3541 tctggcgctc ctcatgatct tcctcagcct cattctcttg atcccatggt gcgaagggat
     3601 acatctcatc tgccaaagga cctaacgccg gaacgtggga gatcttttgg tgcaggtcaa
     3661 ccgagccagc cagatactcg ccgggcaatg taaagaccaa cccaggaccc acactgcgcg
     3721 tgaattgggt gcgcgtgcgc aacaccgcag cgctggcagt atccagcacg atcacacccc
     3781 ggcggtgtgg tttatccctc gggtagcggt cggggatatc accattatca atcttgatag
     3841 ccggtccgtg ccgaccaatg gcgtaggaaa acaggtaatt tgacgccaga atacgctggc
     3901 caaaattgcg cactggaagg gaaaattggg cgaagaaggc aatccaaaac aggaaggcag
     3961 caacaaccaa gaccatatcc cgaaaaaaaa accatcacac cttcgggaat tcgatcccac
     4021 atgagcagga agaacttgac aacccccttg atcagctccc acagcgagaa atcgggagaa
     4081 gctcggttcg ccagatatag gtataggaga atgacgaaca agctgaccag ga
//
LOCUS       DBNHOH010000313         2267 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_338127, whole genome shotgun sequence.
ACCESSION   DBNHOH010000313 DBNHOH010000000
VERSION     DBNHOH010000313.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_338127"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="AC16V7_05610"
     CDS             <1..219
                     /locus_tag="AC16V7_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6256631.1"
                     /translation="EGSGFNADESSHPPIGWGLVGMRERAESVGGSFRIHTSPGIGTQ
                     IEVIVPLSGTEPLPSEENNHESNTLDAR"
     gene            191..880
                     /locus_tag="AC16V7_05615"
     CDS             191..880
                     /locus_tag="AC16V7_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6256632.1"
                     /translation="MSQIRLMLVDDHEIVRTGLRTFLQSQEGFNIVAEASSGKQALER
                     AQEAQPDVVIMDITMPQMDGLEATRRLKRILPDCHVLALTVHEDKQYLFEMLAAGASG
                     YITKQSAAEDLVAAIHSVASGNVYLQPALARWLLEDYRRLLSHAPPATGPASEERKLG
                     KGLEILSERELQVLELLSQGLTNPQIGVKLGISPKTVARHRERIMNKLDMHSSTELVK
                     FAIRTGLIDVN"
     gene            1075..1596
                     /locus_tag="AC16V7_05620"
     CDS             1075..1596
                     /locus_tag="AC16V7_05620"
                     /inference="COORDINATES: protein motif:HMM:NF015085.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HIR6256633.1"
                     /translation="MEESITTTTQTSTAGRAKFIVGGLLIIAAIVYLIVSSTQASAQY
                     FLTVEELNAKVDSVAGRELRISGAVIGDSIQYDPQTLTLTFTVAHVPGDNKEIEAQGG
                     LATALHAAVMDPNRPRLEVVYTGVKPDLLRDEAQAIMTGRMGDDGKFHAEELLLKCPT
                     KYEEAVPQQIDQG"
     gene            1605..>2267
                     /gene="ccsA"
                     /locus_tag="AC16V7_05625"
     CDS             1605..>2267
                     /gene="ccsA"
                     /locus_tag="AC16V7_05625"
                     /inference="COORDINATES: protein motif:HMM:NF013725.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HIR6256634.1"
                     /translation="MAANIGYGALVITFVISLYGIFAAIYGVRKSSPAWVDSARNAML
                     LTWPLLTISALSIIYLLVIGRYEVEYVAEVTSNSMPLYLRITALWGGQAGSLVFWTWL
                     MAAFASAVTLRKWDRDREFLPWVVVVSLVTLAFFLVMIIFFENPFARLWQVPLGQPVK
                     AMLQPEGSVPLVMPDGRGLNPLLRHPGMIIHPPMLYLGFVSFVIPFAFAIAALVTGRT
                     DDR"
BASE COUNT          490 a          625 c          606 g          546 t
ORIGIN      
        1 gagggttcgg gctttaacgc cgacgaaagc tcccacccgc caattggctg gggtctggtg
       61 gggatgcgtg agcgtgccga gtcagtcggc ggatcgtttc gcatccatac atcccctgga
      121 attggcaccc agattgaagt gatcgtgccc ctttcaggca ccgagccatt accgagcgag
      181 gagaacaacc atgagtcaaa tacgcttgat gctcgttgat gaccacgaaa ttgtgcgcac
      241 cggcctgcgc acattcctcc aatctcaaga aggctttaac attgttgcgg aggcttccag
      301 cggcaagcaa gcccttgagc gcgcccagga agcacagccc gatgtggtca tcatggatat
      361 cacgatgccg caaatggatg ggttagaggc aactcgacgt ctcaagagga tactgcccga
      421 ctgtcatgtg ctggcattaa ccgttcacga ggataagcaa tacctgtttg agatgcttgc
      481 ggcgggagct tcgggttaca tcaccaagca gtctgctgcc gaagatctgg tagctgcaat
      541 acactccgtt gccagcggca acgtgtattt gcaacctgca ctggcccgct ggttgttgga
      601 ggactatcgg cggctactat ctcatgcacc accagcgacc ggacctgctt ctgaagaacg
      661 gaagctgggc aaagggctgg aaatcttgag cgagcgtgag cttcaagtgc ttgagcttct
      721 ctcacaggga ctcaccaatc ctcagattgg ggtcaagctg ggcatcagcc caaagacggt
      781 tgcccgccat cgcgagcgta ttatgaataa gctggatatg cactccagca cggagctggt
      841 caaattcgcc atccgtaccg gcctaattga tgtcaattaa agcttgagaa cttctgccgt
      901 tgaagtactt gaatcatcaa ccgtgtagtt tttggttcta atgttttttt tggcgcaatc
      961 tcagctattc ttaatctgaa tttgtaacaa tgtgaattag actattaacc gcaatccaag
     1021 cggcgatttt atgccgccct tagatcattt tactgaactg atgaggtgtt caacatggaa
     1081 gaaagcataa ccacgacaac acagacctcc actgcgggac gcgccaagtt tatcgtaggc
     1141 ggacttttaa ttatcgccgc catcgtgtac ctgatcgtct cctcgaccca ggccagcgcc
     1201 cagtatttcc ttaccgttga ggagctgaat gccaaagtcg atagtgtcgc cgggcgcgag
     1261 ttgcgcatct ctggcgcagt gatcggtgat tccatccaat atgatcccca gacgctgacg
     1321 ctcaccttta cggttgctca cgtgccggga gataacaaag agatcgaggc gcagggtggt
     1381 ctggcaacag cactgcacgc cgccgtaatg gaccccaacc gcccgcgctt ggaagtggtc
     1441 tacactggtg tgaaacctga cctgcttcgt gacgaagcgc aggcaattat gactggacgc
     1501 atgggcgacg atggcaagtt ccacgccgaa gaactcctgc tcaagtgccc gacaaaatac
     1561 gaagaagctg tgccccaaca gatcgatcaa ggttaaactt tatcatggct gctaacatcg
     1621 gatacggtgc gcttgtcatt acatttgtaa tatctttgta cggcatcttt gcagcgattt
     1681 acggcgtgcg caagagctct cctgcctggg tagacagtgc tcgcaacgcc atgctgctca
     1741 catggccttt gcttaccatc tcagcattga gcatcatcta cttgctggta attgggcgct
     1801 atgaggtgga gtatgtcgcc gaggtgacca gcaacagcat gccgctgtac ctgcgcatca
     1861 ccgccttgtg gggcgggcaa gctggctcat tggtcttctg gacctggctg atggctgctt
     1921 tcgcctccgc ggtgactttg cgcaagtggg atcgtgaccg agagtttctg ccttgggtgg
     1981 tcgtggtctc cttggtcacc ttggcattct tcctggtcat gatcatcttc tttgaaaatc
     2041 cgtttgccag gctgtggcag gtacccttgg gtcagccggt aaaagccatg ctccagcccg
     2101 aaggctccgt gcctctggtg atgcctgatg gacgtggcct caatcccttg ttgcgccacc
     2161 ccggcatgat catccacccc cccatgctct acctgggctt tgtctctttt gtcatcccgt
     2221 tcgccttcgc catcgccgcg ctggtcaccg gacgcactga tgatcgc
//
LOCUS       DBNHOH010000314         7484 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_659117, whole genome shotgun sequence.
ACCESSION   DBNHOH010000314 DBNHOH010000000
VERSION     DBNHOH010000314.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7484)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7484)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7484
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_659117"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="AC16V7_05630"
     CDS             <1..354
                     /locus_tag="AC16V7_05630"
                     /inference="COORDINATES: protein motif:HMM:NF021883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIR6256635.1"
                     /translation="VSQRVLSGLQINLSAMRRNLANYAPFAGTERVLMALGKAGADRQ
                     DMHERLREHALAAWQDLQLGGENRLGKLLSSDTEIKRYLQGSEIEALLDASAHVGDAP
                     QRARSLAHTIREAIL"
     gene            complement(349..789)
                     /locus_tag="AC16V7_05635"
     CDS             complement(349..789)
                     /locus_tag="AC16V7_05635"
                     /inference="COORDINATES: protein motif:HMM:NF024773.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR6256636.1"
                     /translation="MDQTIQNFIEDKRLAVVGVSRRGNKFGNMATKELRERGYQVYVV
                     HPEATDIDGEKCYPKLSSLQEKVDNLLVSVPSKQAAGVLQDASSAGIKNIWLQQGSET
                     NELLTQAGQLGLNVVSGKCILMYAPPVRSFHGFHRFVMKLIGQL"
     gene            complement(1040..2032)
                     /locus_tag="AC16V7_05640"
                     /pseudo
     CDS             complement(1040..2032)
                     /locus_tag="AC16V7_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930375.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0008076 - voltage-gated potassium channel
                     complex [Evidence IEA];
                     GO_function: GO:0015459 - potassium channel regulator
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel beta subunit family protein"
     gene            complement(2104..2913)
                     /locus_tag="AC16V7_05645"
     CDS             complement(2104..2913)
                     /locus_tag="AC16V7_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR6256637.1"
                     /translation="MISTLQFATELACSTGKLLTEFYRPGGSEARLKADQSVVTEADL
                     AADRLVKQAIRDAYPQDLLLSEELHPSLSVGNQEAVWVVDPLDGTTNFSLGLHIWGVS
                     IARAVNGIPETGAIYFPLLDELYSAQIGTGAFWNGERIRVQPMDKEKPATFFACCGQT
                     HKLYNVSVPYKPRILGSAAYDFCALARGAAVLTFEATPKIWDLAAAWVLILEAGGVIE
                     SHHGPAPFPLTPGMDYTKRAFPTLAAATAKMVVKGRNMIKIKDELLLTNDG"
     gene            complement(2910..4889)
                     /locus_tag="AC16V7_05650"
     CDS             complement(2910..4889)
                     /locus_tag="AC16V7_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6256638.1"
                     /translation="MSITADLILTNAIVLTMDEKFAHYEPGAVVVNGDSITAVGPDAE
                     IRAAYSAKETLDCGGKVLMPGFVNAHTHVPMTLLRGLADDLRLDVWLMGYMMPVEREF
                     VSPDFVRLGTKIACVELIRSGVTSFADMYYFEEEVARATAEAGLRAVCSQTVMKYPTP
                     DAQFYEESLASARDFLEKWKGHPLIVPSVAPHAPYTCTPDILNATAQLAVEFDVPLHT
                     HLSETAGEVENSRDEHGMPVIPYVKKQNLFDAKVLAAHCVHVDEGEIYTLLHHHAGIA
                     HNPSSNLKLASGFAPVSKMLEAGLNVGIGTDGPASNNDLDMFEEVRLAAFLAKAHSND
                     PTVLPASSALAMATRLGANALHIGSLTGSLEPGKRADFILVDLSPLHNAPRFRRDPNG
                     LYAQLVYAGKSTDVTHVMVNGQWLMRDHQLTTLDESDLLTQAAEYASKIDYFLIQREQ
                     SVLSKLIAIGGATEAESFEVQAKVRISDTSSVHQALEKPEIEILHHRHYREYDTYFSF
                     EDPKQGHLRYREDEFIDSKGEVSNVRYRLTLLGPAREEHFPSDVLLSRSRFIAPATHS
                     LRFYREYFKPTSETAIQKDRLRWRILFRGTEFYINLDFVNTPDLGTFLEVKSRTWSRR
                     DAEFKAELATDIIIFLGALPEKTVAQDYIEIVEDR"
     gene            complement(4906..6048)
                     /locus_tag="AC16V7_05655"
     CDS             complement(4906..6048)
                     /locus_tag="AC16V7_05655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HIR6256639.1"
                     /translation="MPCAEIITIGTEILLGDIVDTNAAYLARALRDAGIDLYRKTTVG
                     DNVRRIAQAIQQSLERCDIILTTGGLGPTVDDPTREAVALAFGVETEFLPELWEQIQA
                     RFRRYNRQPTENNKRQAYVPRGATPMENPVGTAPAFAVEKGDRVVISLPGVPREMEYL
                     VQNAVLPYIKQRFNLQGIIKSRVIHTAGVGESQIDDLIGDLEALSNPTVGLAAHSGQV
                     DVRITAKAASESEADALIQPIEVTLRQRLGKWVYGADQETLEAAALENLQRKGWSLAV
                     VEAGLAGNLIRRLASAQGPFLGGQVLPHSPTPEELTSLTDAYRLSCSAEIGLGVAIYP
                     AGDRQDVYLTLVSPDGIQEITRPYGGPKEYAPRWALHHSLDVIRNL"
     gene            complement(6108..7262)
                     /locus_tag="AC16V7_05660"
     CDS             complement(6108..7262)
                     /locus_tag="AC16V7_05660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6256640.1"
                     /translation="MIVPKLADIVIIGGGVMGASIAYHLALHGQDNIVLLEKEDFFGQ
                     GATGRCAGGVRYQFATEVNIRLSLQSLPMLERFEDEIGQAIDYRQCGYLFVLTQPADV
                     EVFKRNVALQNQLGAGTIWLDGDEIRRRLPLMHMEDVIAGTFNPKDGLVDPNGVVMGY
                     IRSAKRRGVQALTGMEVIGVKVVGGQVEGVETSAGVIATRVVINAAGPWAGLVGRMAG
                     VDIPITPIRRQMFTTTPIPEIPPDFPFVIDFAQSLYFHRESQGLLIGMSNPNEKPGFD
                     QNVDPEFELVNLESATARLPLLEHAGLASHWAGLYEVTPDAHPIYGKTPIEGFYTCTG
                     FSGHGFMHGPISGKLMAEILLDGAAHSVDISMLDLARFEEGRLIREYNVV"
     gene            complement(7273..>7484)
                     /locus_tag="AC16V7_05665"
     CDS             complement(7273..>7484)
                     /locus_tag="AC16V7_05665"
                     /inference="COORDINATES: protein motif:HMM:NF014476.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HIR6256641.1"
                     /translation="LKAILLGYITAKHIHGELGEIVLGRKPGRTNPDQVTFFKSVGVA
                     VQDAVAARLALENAQKNGLGQQVEW"
BASE COUNT         1713 a         1983 c         1937 g         1851 t
ORIGIN      
        1 gtttcacagc gtgtcctgag tgggttgcag atcaacctgt ctgcgatgcg ccgtaatttg
       61 gctaactatg caccgttcgc ggggactgaa cgggtgctga tggctctggg gaaggctggc
      121 gccgatcggc aagacatgca tgaacgcctg cgtgagcatg ctctggcagc ctggcaagac
      181 ctgcaattgg gtggggagaa tcgtctcggt aagctattga gcagcgatac agaaattaag
      241 cgttatctac agggaagcga gatcgaagcg ctactggatg ccagcgccca cgttggcgat
      301 gcgccacaac gcgcgcggtc gctggctcat accatacgcg aagcaatatt atagctgccc
      361 gattagcttc ataacaaagc ggtggaaacc gtgaaaagag cgtaccggag gggcatacat
      421 caggatgcac ttgccactga ccacattcaa ccccagctga ccagcttgcg ttagcaactc
      481 gttggtctcc gacccctgtt gaagccatat attcttgatg ccagctgatg atgcgtcttg
      541 tagcacgccc gctgcttgct tggaaggtac gctgaccaag aggttatcta ccttttcctg
      601 caaagagctc agtttaggat agcatttttc cccatcgata tctgtagctt cgggatgcac
      661 aacgtatacc tggtaaccgc gctccctgag ctctttggtc gccatgttgc cgaacttatt
      721 tcccctgcgc gaaaccccca ccactgccaa acgcttgtcc tctataaagt tttgtattgt
      781 ttgatccatc ttctatcctc ctgcgttctc tatgcagtta ggtatcgctt cctactgcag
      841 gaacaaaatt ttacttatat tagcaatatt tttactctat gtgtactgtt tttcgtcacc
      901 cggggacgcg agcaatatca tgccaaccct aattcgggga gggatagata tatgtgatcg
      961 gtgatatggc ttttcgtatt tggttctttt ttagagttgt ttctaggttg gtgatgatct
     1021 cggtaatatc gaggtttatt tatcgctcat cctctattgg ctcaggcttg ttgtcgagaa
     1081 tctcctcgat acgttccatg atatcaggtg tgagctttcc aactacctcc aaggatccga
     1141 gattctcact tatctggtct gggcgagatg cacctgtgat taccgtactc acatgttgat
     1201 tcttgagaca ccatgctaag gctagttgtg ccatgctgca gcccagctca tcagcgagtg
     1261 gtgctaactg ccgagcttta gcaatgtttt gttggctttc ttcgctttca atccactctt
     1321 ttagccattc atatcccttc aggttagccc gtgttccagg gggaagacct tcgttgtact
     1381 tcccagtaag cagcccgcta gctaatgggc tgaagatggt ggtccccaag cctgtctcat
     1441 cgtatagcgg agcatactct ttctctacgc gctcgcgatg gaacatgttg tattcgggct
     1501 gctccatcag cggaggtatc aaatgctcgc ggcgggcgat tcgataggca tccaagatct
     1561 ggtatgcgct ccactcgctg gtaccccaat atagagcctt cccctgttgg atagcctggt
     1621 tcatcgccca gactgtctct tcaatggggg tgtagatgtc gggtcggtgg caaaaaacca
     1681 ggtcaacgta gtccatctgg agcctttata acgaagcgtt caggccttcg aagatgtgtt
     1741 tgcgagataa acccctgtca ttcggtcctt tgcctcccca aaagattttg gttgagataa
     1801 cgagatcttt gcgtttccaa tcagcttttt ttatcacatt ccccatcgta gtctcagcct
     1861 tgccatgggc ataggcttcg gcattatcga agaaattgat gccggcatca taggccagct
     1921 tcatacactc aaaggccaca tcgtcgctga tctgttgtcc aaaggtgacc caggctccca
     1981 atgagagagc cgaaattttt agtcctgact ttcccagaaa gcggtattcc atattagcat
     2041 tctccaagtg atgtgaaatt tactagtttc tagaactgat gtattcaccg ctgatggatg
     2101 gtatcatccg tcgtttgtta acagcagttc gtctttgatt ttaatcatat ttctaccctt
     2161 caccaccatt ttagcagttg ctgctgccag ggtgggaaaa gcccgtttgg tgtaatccat
     2221 gccaggcgtc aatggaaacg gtgcaggacc atgatgagat tcaatcactc caccggcttc
     2281 aagtatcagc acccaggctg ccgccaggtc ccaaatctta ggtgtggcct caaacgttag
     2341 cacggcggca ccgcgcgcta atgcacaaaa gtcgtaagca gctgagccta atatgcgcgg
     2401 tttataggga acactaacgt tgtagagctt atgggtttgc ccgcagcaag cgaagaatgt
     2461 agctggtttt tctttatcca ttggttgaac ccggatgcgc tccccattcc agaaagctcc
     2521 cgtgccaatt tgggcggaat aaagctcatc taacaaaggg aagtaaatgg ccccggtttc
     2581 aggtataccg ttcacggcgc gtgcgatgga aaccccccaa atatgcaacc ccaagctaaa
     2641 gttggttgta ccgtccagcg gatcaaccac ccagacagcc tcttggtttc caacagacaa
     2701 cgatgggtgg agttcttccg ataagagcaa atcctgtgga taagcgtctc ggatagcctg
     2761 tttcaccaag cggtcggctg ccagatcggc ttccgttacc acagactggt cagccttcag
     2821 gcgtgcctct gatccaccag gccggtaaaa ttctgttagc aacttcccgg ttgagcaggc
     2881 gagctctgta gcgaactgga gtgtgctgat catcgatctt cgacgatttc gatataatcc
     2941 tgcgctacag ttttttcggg caatgctccc aggaatatga ttatatcagt cgcaagctcg
     3001 gctttaaatt cggcatcccg ccggctccac gtgcggctct ttacttccag aaaggtgcct
     3061 agatcagggg tgttgacaaa atcgagatta atatagaatt cggtgcctcg gaataagatc
     3121 ctccagcgca agcggtcttt ttggattgcc gtctcagaag tcggcttgaa atactcccgg
     3181 tagaaacgca agctatgtgt ggctggcgcg atgaatcggc tgcgcgagag caatacgtcg
     3241 ctgggaaaat gctcttcgcg cgcagggcct agcagagtca gacggtagcg cacattgctg
     3301 acttcacctt tagagtcgat aaactcatcc tcgcggtagc gaagatgccc ttgtttggga
     3361 tcttcaaagg agaaataggt atcgtattct cggtaatggc ggtggtgcag gatctcgatc
     3421 tcgggctttt ccagtgcttg atgtactgag gatgtatcgg aaattcgcac tttagcctgc
     3481 acctcaaagc tttctgcctc ggtcgctcca ccgatggcga tgagcttgga gagcaccgat
     3541 tgctccctct gaataagaaa ataatcaatt ttagaggcat attctgcagc ttgcgtcagc
     3601 agatcggact catcgagggt agtcagctgg tgatcgcgca tcagccattg accattgacc
     3661 attacatggg tgacatcggt cgacttgccg gcataaacca actgtgcgta cagtccgttg
     3721 gggtcgcggc gaaagcgggg tgcattatgc agtggggaga gatccaccag gataaaatcg
     3781 gcgcgtttgc caggttccag cgagccggtc aggctgccta tgtgaagggc atttgcgccc
     3841 aggcgtgtag ccattgccag ggcagacgag gcgggcaaca cggtaggatc gtttgagtga
     3901 gctttggcca agaaagctgc caggcgcact tcttcaaaca tgtccaggtc attattggag
     3961 gctggcccgt cggtgcctat gccaacattt agaccggctt ccaacatttt gctcaccggc
     4021 gcaaaacctg aggctaactt gaggttagaa gatggattat gggctatacc cgcatgatgg
     4081 tgtagtagag tgtaaatctc cccctcatca acatgaacgc aatgagccgc aagcactttg
     4141 gcgtcgaaca ggttctgttt cttgacatag gggatgactg gcatgccgtg ctcatcgcgt
     4201 gagttttcca cttcgccggc ggtctcagac aaatgagtgt gcaagggtac atcgaactca
     4261 acagccagct gggcagtggc gttcaggatg tcgggtgtgc atgtatacgg ggcgtgcggt
     4321 gctaccgaag ggacaatgag tggatgacct ttccactttt caaggaaatc acgagcagag
     4381 gctagggatt cttcatagaa ttgagcatct ggtgtgggat atttcatcac agtttgtgaa
     4441 caaactgcgc gcagcccagc ttcggcggtt gcccgggcga cttcctcttc gaaataatac
     4501 atgtcagcga agctggtcac acccgagcgt atcagctcga cacaggcgat cttggtgcca
     4561 agacggacaa aatccgggga aacgaattcg cgttcaaccg gcatcatgta ccccataagc
     4621 caaacatcta atcgcagatc atctgccagg ccgcgcaaca gggtcatggg aacgtgggta
     4681 tgggcgttga caaagcctgg catcagcact ttaccgccgc aatccagcgt ctctttggcg
     4741 gagtaagccg cccggatttc cgcatcaggc ccaacagcgg taatgctgtc accgttgact
     4801 accactgcac cgggttcata gtgagcaaat ttttcgtcca tggtcaggac gatggcattt
     4861 gtgaggataa gatctgcggt tatggacaca aaatcacctt tctagtcaaa gattacgaat
     4921 tacatccagg ctgtgatgca gtgcccagcg cggggcatat tctttcgggc cgccgtatgg
     4981 gcgggttatc tcctggattc catctgggga aaccagggtt aggtacacgt cttgacggtc
     5041 gccagctggg tagatcgcaa cacccaaccc aatctcggcg ctgcaagaca ggcggtaggc
     5101 atccgtcaag gaggttaatt cctcgggtgt tggggagtgt ggcaacacct ggcctcctag
     5161 gaaaggccct tgagctgaag ccaacctgcg gattaaattg ccagccagcc ccgcctcaac
     5221 cactgccaag ctccaacctt tacgctggag attttccagg gcagctgctt ccagggtctc
     5281 ttgatctgca ccatataccc acttccctaa gcgctgccgc agagtaactt caatgggttg
     5341 gatgagagca tcggcctcac tttctgatgc agcttttgcg gtgatgcgca catccacttg
     5401 tccagagtgg gctgccaagc ccacggtggg gttacttagc gcttctagat cgccaatcag
     5461 atcgtcgatc tgggactcac ccacacccgc agtgtggata actcttgact taatgatacc
     5521 ctggagattg aagcgctgct tgatgtaagg caagacagcg ttctgaacca gatactccat
     5581 ctcccgaggc acgcccggca gcgagataac cacccgatct cccttctcta ctgcgaaagc
     5641 cggcgctgtc cctaccggat tttccattgg tgtggcgcct ctgggtacat aagcctggcg
     5701 tttgttattc tcggttggct ggcggttgta acgccggaag cgagcctgga tctgctccca
     5761 taattcgggg aggaactcag tctccacgcc aaatgccagc gcaaccgcct cccgggtcgg
     5821 atcatccacg gtgggtccca acccccccgt ggttaggatg atatcacagc gttccaggga
     5881 ttgttgaata gcctgggcga tacgtcgcac gttgtcgcca accgtcgtct tgcggtacaa
     5941 gtcaataccg gcatcacgca gggcacgagc gagatacgca gcgttggtat ctactatgtc
     6001 gccaagtaat atttcggtcc caatggtgat gatctctgca cagggcatat tctaactgcg
     6061 gccaggctta gggtctacat gaaggtgttc ggcttatgga tcagccttca aactacatta
     6121 tattcgcgaa tcaagcgacc ttcttcgaaa cgggctaggt cgagcataga gatatctaca
     6181 ctatgggctg caccgtccag cagaatctcg gccattagct taccagaaat cggcccgtgc
     6241 ataaagccgt gccctgaaaa cccggtgcat gtgtaaaaac cctctatggg tgttttgcca
     6301 tagatcggat gtgcatctgg ggttacctca tataaccccg cccagtgcga tgccaatcca
     6361 gcatgctcca gcaggggaag gcgagcggtg gcactttcaa gattcaccag ctcaaactct
     6421 ggatcgacat tctggtcgaa acctggcttt tcattggggt tggacatgcc aatcagcaac
     6481 ccctgacttt cgcggtgaaa atacaatgat tgggcaaaat caattacaaa ggggaaatcg
     6541 ggagggattt ctgggatggg ggttgtggtg aacatttgcc gtctaatagg ggtgatcgga
     6601 atatccacac cagccatgcg acccaccaac ccggcccaag ggccagccgc gttgattacc
     6661 acgcgtgtgg cgatgacccc agcggaagtc tccacccctt caacctggcc tccgactacc
     6721 ttaactccaa tcacttccat gccggtcaac gcctgcacac ccctgcgctt agcggagcgg
     6781 atatagccca tgaccacacc gttgggatca accaaaccat ccttggggtt aaaagtaccc
     6841 gcaatcacat cttccatgtg catgagaggc aaacggcggc ggatttcatc gccatccagc
     6901 cagattgttc ccgctcctag ctggttttgc agcgctacgt tacgtttgaa gacctccaca
     6961 tctgcaggct gagtgagcac aaatagatac ccacactggc ggtaatcaat cgcctgaccg
     7021 atctcgtcct cgaagcgttc cagcataggc aggctttgca atgacaggcg gatattcact
     7081 tcggtcgcga actggtagcg caccccaccc gcgcaacgac cagtagcacc ttggccgaag
     7141 aaatcttctt tttcgagcag gacgatattg tcctgtccat gcagagccag gtgataagcg
     7201 atgctggcgc ccatcactcc acctccgatg atgacgatgt cggctaattt tgggactatc
     7261 ataattaccc ctttaccact ccacttgctg ccccaaccca ttcttctgag cattttcaag
     7321 cgccaggcgg gcagccaccg cgtcctggac tgccaccccg acagatttga agaatgtcac
     7381 ttgatctggg ttggttcgac caggtttgcg gcctagcacg atctcgccga gttcgccgtg
     7441 gatatgtttg gcggtgatgt aacccagaag tattgctttt aaca
//
LOCUS       DBNHOH010000315         1885 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1685349, whole genome shotgun sequence.
ACCESSION   DBNHOH010000315 DBNHOH010000000
VERSION     DBNHOH010000315.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1685349"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /gene="sufC"
                     /locus_tag="AC16V7_05670"
     CDS             complement(<1..642)
                     /gene="sufC"
                     /locus_tag="AC16V7_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HIR6256642.1"
                     /translation="MSELSIRNVHVSIDGKEILKGVDLTIRQGEIHALMGPNGTGKST
                     LAYALMGHPSYVITDGEVWFKGQNILELEPDQRSHLGIFLAFQYPVAIPGVSVANFLR
                     SALNARRKAINPEDKGVPIPEFRKLLKERMDLLKMDHSFAGRYLNEGFSGGEKKRAEI
                     LQMAALRPEIAILDETDSGLDIDALRIVSEGVNALRSEELGVLVITHYQRILNY"
     gene            842..1540
                     /locus_tag="AC16V7_05675"
     CDS             842..1540
                     /locus_tag="AC16V7_05675"
                     /inference="COORDINATES: protein motif:HMM:NF013209.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR6256643.1"
                     /translation="MTEFDPHAILFRMATIVRDEEQSKSTRQRVMQTLLSRQRCTIND
                     LAEAVEINPISVRHHITRLEAEGLVNSEEERHGVGRPRRLYFLTEKGLERFPSRYLRF
                     TIRLLEQLKDTMPEAMVSSLFRQMAKDVAADYESMVNGLNMEERIDLVAQLLSSEGFT
                     VEWERKGSEYHIREISCPYYHIGQSHPEVCSVDQTLISTMLSVPAEKVKCVLNGDTNC
                     TYVVSSMASLENNQ"
     gene            1537..1881
                     /locus_tag="AC16V7_05680"
     CDS             1537..1881
                     /locus_tag="AC16V7_05680"
                     /inference="COORDINATES: protein motif:HMM:NF013999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HIR6256644.1"
                     /translation="MSEETSSTLIWQVDSTHPELSAPLRDALREVVDPEIGLNIIELG
                     LVRDVAVEEDSAKITMILTTPFCPYGPALLDITRQKGEKALQRSTTIEMGIEMWDFSM
                     MEEGAGAAWGFS"
BASE COUNT          533 a          462 c          420 g          470 t
ORIGIN      
        1 atagttcaaa atacgctggt agtgtgtaat tacgagcacg ccaagctcct cgctgcgcaa
       61 tgcattaacg ccctcagaga caatccgcaa cgcatcgata tccaagcctg aatctgtctc
      121 atccaaaata gcgatctcgg ggcgcaaggc ggccatctgc aagatctctg cgcgcttctt
      181 ttccccacca gaaaatccct catttagata acgcccggca aagctgtgat ccattttgag
      241 tagatccatg cgttccttca aaagcttcct aaattcagga atcgggacgc ctttatcctc
      301 tggattaata gccttgcgcc gagcattcaa tgctgatcgc aagaaatttg ctactgacac
      361 acccggtatt gccacaggat actgaaaagc cagaaaaatc ccaagatgtg aacgctggtc
      421 cggttccagt tcaaggatat tttgaccttt gaaccagact tcgccgtcag tgattacata
      481 gcttgggtgt cccattagcg catacgccaa ggtggatttg ccagtgccat ttggccccat
      541 taaagcgtgg atctcgccct gtcttatggt cagatcgaca cctttcaata tttccttgcc
      601 atcgatgctc acgtgcacat tgcggatgga aagttcagac ataaataaag ctccttatcg
      661 tatgtgatta caaattcgac caggggtgat aataattcga tttgaatgca cccatccaaa
      721 tgcagtatta tagcagtcct gggccaatcc gtcaatattt agcataaaaa tcttatatat
      781 tcacgattat agcaaggcgt acttcaaaag tcaataatat ttagcataaa aatcttatat
      841 attgacagaa ttcgacccac atgctatact ttttcgcatg gcaaccatag ttagagatga
      901 agaacaatct aagagcactc gccagcgtgt aatgcagaca ttactcagca gacaacgctg
      961 cacaatcaat gacctggcag aagcggttga gatcaacccc atatcggtac ggcatcatat
     1021 cacccggctg gaagcagagg gtttagtcaa ttcggaagaa gaacgccatg gtgttggacg
     1081 accacgacgg ttgtatttcc ttacagaaaa gggcttggag cgctttccat cacgttacct
     1141 ccgctttacc ataaggttgc tggaacagct aaaagacacc atgccagaag cgatggttag
     1201 cagccttttc cggcaaatgg ctaaggacgt tgccgctgat tatgaatcaa tggtcaacgg
     1261 gctcaacatg gaagaacgga ttgacctggt tgcccaactg ctcagcagtg aaggtttcac
     1321 cgtcgaatgg gaacgcaaag gcagcgaata ccatatacgc gagatcagtt gcccgtacta
     1381 ccatattggg caatctcatc ccgaggtttg ctctgtcgac cagactctca tttccactat
     1441 gctttccgtg ccagcagaaa aggtcaaatg cgttttgaat ggcgatacga actgcacata
     1501 tgtagtatcc agtatggcct ctttagagaa taatcaatga gtgaggaaac cagctcaaca
     1561 ctaatttggc aagtcgacag cacccatccc gaattatccg ctcccttgcg tgatgcgcta
     1621 cgagaagttg tcgacccaga aattggatta aatatcattg agcttggttt ggtgcgtgat
     1681 gtggcggttg aagaagattc tgcaaaaatc accatgatcc tcaccactcc gttttgtccc
     1741 tatggaccag cactactgga catcacccgt caaaaaggcg aaaaagccct gcaacgctct
     1801 actaccatag aaatgggtat agagatgtgg gatttctcca tgatggaaga gggagctggc
     1861 gcggcctggg ggttttccta aacct
//
LOCUS       DBNHOH010000316         1376 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_467106, whole genome shotgun sequence.
ACCESSION   DBNHOH010000316 DBNHOH010000000
VERSION     DBNHOH010000316.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_467106"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="AC16V7_05685"
     CDS             <1..291
                     /locus_tag="AC16V7_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256645.1"
                     /translation="IVTAAAAPSAVLDSLLPGFYSIVIPALGTLFASLAMLKGVFSKP
                     TAYLGLAVGVTGIIAVADPFIGALGKVHIINALLATIWFLLVGIRLYKLGQQ"
     gene            366..1343
                     /locus_tag="AC16V7_05690"
     CDS             366..1343
                     /locus_tag="AC16V7_05690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HIR6256646.1"
                     /translation="MEVTKPEPKEDQVLVKVHAASANALDYRRFEKISTMGRLMDERL
                     TKSVGKVLGADISGVVVAVGAKAKKFQPGDEVFGVAAGSKGGFAEYACAAEDHLALKP
                     ASLSFETAAAVPVAAMTALQGLRDKGQIQPGQKVLINGASGGVGTFAVQIAKSFGAEV
                     TAVCSTRNLEIARSIGADYVIDYTKEDFTKSGERYDLIFAVNGNHQLSVYQRALNPQG
                     VYICAGGALPQIIQAMLLGPLVSIIARKKMGFMGIAKPNQKDLIYMAELLEAGKVVPV
                     IEKCTPLNEGPAVMRYLAEGHATGKVVLIVDHNHRIRHGPDQNQLGGDS"
BASE COUNT          329 a          346 c          383 g          318 t
ORIGIN      
        1 atcgtcactg cggctgccgc tccgtccgcg gtgctagatt ctcttctgcc aggcttttac
       61 tccatcgtga tcccagcgtt gggaacgctc tttgcgagcc tggccatgct caagggggtt
      121 ttcagcaagc ctacggctta cctgggcctg gctgtgggtg tcaccgggat cattgctgtg
      181 gcagacccat tcataggcgc attaggtaag gtgcatatca tcaatgccct ccttgccaca
      241 atctggttct tgctggtagg catacgactc tacaagctcg gtcagcagtg actcctattc
      301 cacggaggaa taaaataaaa gcaattgttt acacccaata cggtcccccg gatgcccttc
      361 agctcatgga ggtcacaaaa cctgagccca aggaggatca agtcctggta aaagttcatg
      421 ccgcatctgc caacgccctg gactaccggc gattcgagaa gatatcgacc atgggccgcc
      481 tcatggatga acgtttgacc aaatcggtcg gcaaggtact gggtgcggac atatctggag
      541 tagttgtggc ggttggtgca aaggcaaaaa aattccagcc tggtgatgag gtcttcgggg
      601 tagccgcagg gtcaaaaggt ggatttgccg agtacgcgtg tgctgctgaa gatcatttag
      661 ccctgaaacc ggccagtcta tcatttgaga ccgccgctgc cgtccctgta gcggcaatga
      721 ccgccctgca gggtcttcgc gacaagggac agattcaacc tgggcaaaag gttctcatca
      781 acggcgcgtc tggtggcgta ggcacatttg cggtgcagat tgccaaatcc ttcggggctg
      841 aagtgactgc cgtgtgcagc acgcggaacc tggagattgc gcgctcgatt ggtgcagact
      901 acgtcattga ttacacaaaa gaagatttca cgaaaagtgg tgagcgttac gacttgattt
      961 ttgctgtcaa tggaaatcac cagctttcag tctatcagcg cgctctgaat ccccaaggag
     1021 tgtatatctg cgcaggaggc gccttgccgc aaataattca agcgatgcta cttgggccat
     1081 tggtatcgat cattgcaagg aagaagatgg gcttcatggg aattgcgaaa cccaaccaga
     1141 aggatttaat ttacatggca gagcttctcg aagctggcaa agtcgttccg gtcatcgaaa
     1201 aatgtacccc cttgaatgaa ggcccggcgg tgatgcggta tcttgctgaa ggacacgcca
     1261 ctggaaaagt tgtcctaatt gtggatcata accacagaat ccgacacggt cccgatcaaa
     1321 atcaacttgg aggtgactcg tgaaagcgac tgcgtatgca agtgtggggt cacgcg
//
LOCUS       DBNHOH010000317         1530 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1044276, whole genome shotgun sequence.
ACCESSION   DBNHOH010000317 DBNHOH010000000
VERSION     DBNHOH010000317.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1044276"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /gene="hemH"
                     /locus_tag="AC16V7_05695"
     CDS             complement(<1..845)
                     /gene="hemH"
                     /locus_tag="AC16V7_05695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - protoporphyrin ferrochelatase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HIR6256647.1"
                     /translation="MGLTPQRSIAVLLMAYGSPDSPDQMEAYLLDVRGGRPTPQTLVD
                     EISHRYAQIGGRSPLLDLTRRQADALEAELNRRLEAENIRFHTYLGMRHWEPRIAEGI
                     AQIAADGLTDVVALVMAPHSSRMSTGMYFKRLEEAVAEQGLNLQIFPITSWHNHPGLI
                     EAITSQALDAIEKFGDQAPYIVFTAHSLPERILAQGDLYDVQLRETAAMVAARLAWPP
                     QRWEFCYQSAGQSAEPWLGPQIEQEVVRLADGGEKNLLIVPIGFVCDHVEVLYDIDIA
                     ARQLA"
     gene            complement(836..>1530)
                     /locus_tag="AC16V7_05700"
     CDS             complement(836..>1530)
                     /locus_tag="AC16V7_05700"
                     /inference="COORDINATES: protein motif:HMM:NF018484.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HIR6256648.1"
                     /translation="WMPEQTPRTLSHFAFYRFSKQLYLLPSDERKQILSAWLALISAT
                     ASKVDVYQLYPTQADIDVLVWSAIEADQPDQAHDFFSDFARQTNPHRHYIHPVQTLWG
                     FTRPSQYTKTRSTQEIDPFATERKPYLVIYPFVKTTDWYLMGREARQGMMNEHIRIGK
                     QYPEITQLLLYSFGLQDQEFIVVYETADLVQFSDLVYELRASEARRYTLRDSPLYTAI
                     YHPPQETLALWA"
BASE COUNT          336 a          392 c          468 g          334 t
ORIGIN      
        1 tcggctaatt gtcgggcggc aatatcaatg tcgtagagca cctcgacatg gtcgcagaca
       61 aaaccgatgg ggacgatcag aaggtttttc tcgcctccat cagccagccg gacgacctcc
      121 tgctcgatct gtggtcccag ccagggttcg gcgctttgtc cagcactctg gtagcagaat
      181 tcccaacgct gagggggcca ggcaaggcgc gcggcgacca tcgctgcagt ctcgcggagt
      241 tgaacatcgt ataggtcgcc ttgagcgagg atgcgctctg gcaggctatg agcagtgaag
      301 acgatatacg gtgcttggtc gccaaatttc tctatggcat ctaaagcttg cgaggtaatg
      361 gcttcaatca agccaggatg gttatgccag cttgtgatgg ggaaaatctg caggttcaag
      421 ccttgttcgg cgacagcttc ttccaagcgc ttgaaataca tgcctgtgct catgcgcgag
      481 ctgtgcggcg ccatgaccag ggcgaccacg tccgtcaacc cgtcggctgc aatctgggcg
      541 ataccctcgg caatgcgcgg ctcccaatgg cgcatgccga gatacgtgtg aaagcggatg
      601 ttttctgcct caagtcggcg gttgagctct gcctccaagg catcagcctg gcggcgagtg
      661 aggtccagaa gaggcgaccg cccaccgatc tgggcatagc ggtgcgaaat ctcatcaacc
      721 agggtctgtg gcgtggggcg gccgccgcgc acgtccagca ggtaagcttc catctggtcg
      781 ggcgagtcgg ggctgccata ggccatcaac agcacggcaa ttgatcgttg tggtgttagg
      841 cccatagcgc cagcgtctcc tgtgggggat ggtagatcgc ggtgtacagg ggagaatcgc
      901 gcagggtgta gcgcctcgcc tcgctggcgc gcagctcgta taccaaatca gagaactgca
      961 ccaggtctgc ggtctcatag accacgataa attcctgatc ctgcaggccg aaagaataca
     1021 gcagaagctg cgtgatctca gggtattgct ttccgatgcg aatgtgctcg ttcatcatac
     1081 cctggcgcgc ttctcgtccc atcaggtacc aatcagtggt tttgacgaat gggtagatca
     1141 ccaggtaagg cttgcgctcg gtagcgaatg ggtcaatttc ctgagtcgaa cgggttttcg
     1201 tatactggga agggcgagtg aatccccaca aggtttgcac aggatggata tagtgccgat
     1261 gaggattggt ctggcgggcg aaatcagaga aaaaatcgtg ggcttgatca ggttggtctg
     1321 cttcgatggc gctccaaacc agcacgtcaa tatccgcctg ggttggataa agctgatata
     1381 cgtccacttt tgaagcagtg gctgaaatca gagccaacca tgcagaaagg atttgtttgc
     1441 gttcgtctga ggggagaagg tagagttgtt tgctaaaccg ataaaaagcg aaatggctca
     1501 atgtgcgcgg cgtctgctca ggcatccaac
//
LOCUS       DBNHOH010000318         1660 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_274406, whole genome shotgun sequence.
ACCESSION   DBNHOH010000318 DBNHOH010000000
VERSION     DBNHOH010000318.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_274406"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..463
                     /locus_tag="AC16V7_05705"
     CDS             158..463
                     /locus_tag="AC16V7_05705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR6256649.1"
                     /translation="MLIVHVFVHVKQEFVDDFRAATILNARNSVQEAGIARFDVIQQQ
                     DNPTRFVLVEVYRTADDPARHKETAHYATWRDTVAEMIAEPRTSIKYTNIFPDESGW"
     gene            478..1632
                     /locus_tag="AC16V7_05710"
     CDS             478..1632
                     /locus_tag="AC16V7_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR6256650.1"
                     /translation="MQFEFATATRIIFGKGEIGNAGVLAASMGRRALITAGRDQSRAE
                     LLVGVLKESGVQTTYFAVSGEPTTALVRQGVELARQEGCDLVIGFGGGSAIDAGKAIA
                     ILLTNEGDVYDYLEVIGKGKSFGRPSAPFVAIPTTAGTGSEVTRNAVLSAVDFGVKVS
                     LRSSWMLPQLALIDPSLTYSLPPSITAFTGLDALTQLIEPYLTKNPNPLTDTICREGM
                     QRAARSLLNAYNNGKDEAAREDMSIAALFSGLALANAKLGAVHGFAGVLGGSLNAPHG
                     AICARLLPVVMSINARALSERAPQSEAFARCDEVAQILTGKANARAADGIAWLYDLVA
                     GLGTAPLSSFGLRRNDFPGIVQKSAKASSMKGNPVQLTQEEMSEILELSL"
BASE COUNT          415 a          357 c          475 g          413 t
ORIGIN      
        1 gccaagcttt ttaatgtttg gttgggaatt gggtatacct cctttgaccc atgcgaagct
       61 tgtattgctt agtataaacg gctcttccct gacgcgtcaa ttgaatttag agaaggattc
      121 agatatacta ccttaaacat ttaacgagga ggccaagatg ctcattgtgc atgtattcgt
      181 ccacgtcaaa caagaattcg ttgatgactt tcgagctgca acgattttaa atgctcgtaa
      241 tagcgtgcag gaggctggta ttgcgcgttt cgatgttatc cagcagcagg ataaccccac
      301 ccgttttgtc ttggtggaag tgtatcgcac cgcagatgac cctgctcggc ataaagagac
      361 agcccactac gcaacgtggc gagatactgt ggctgagatg atagcagaac cgcgcacgag
      421 catcaagtat acaaatatat tccctgacga atcgggctgg tgaacatggg gaattccatg
      481 cagtttgaat ttgccactgc gacacgcatc atattcggta aaggggaaat tggtaatgct
      541 ggagtcctgg cggcgtctat ggggcgccgc gccttgatca cggctggacg agatcagagc
      601 agggcagagc ttctagtggg ggtgttgaag gaatcaggag tccagaccac atatttcgca
      661 gtaagcggtg agccgacgac ggcactggtt cgacaggggg tggaactagc ccgacaggag
      721 gggtgtgatc tagtgattgg ttttggtggg ggaagcgcaa tagatgctgg taaagcaatt
      781 gctatcttgt tgaccaacga aggcgatgtg tatgattatt tggaggtaat aggcaaaggc
      841 aaatctttcg gcagaccatc tgcgcctttt gtcgccatcc cgaccacagc aggcacaggc
      901 agcgaagtca ctcgcaacgc agttttaagc gcagtcgact ttggggtgaa ggtcagcttg
      961 cgttcatcgt ggatgttgcc gcagctggca ttgattgacc cgtcgctaac ctatagcctg
     1021 cctccaagta tcacagcctt cactggtctg gatgcgctaa cccaattgat tgagccgtat
     1081 ctaacgaaaa atccgaaccc attaaccgat acgatttgca gagaagggat gcaacgtgca
     1141 gcccgttcgc tgttgaatgc ttataataat gggaaggacg aggctgcacg agaggatatg
     1201 tcgattgcgg ctttatttag cgggcttgca ttagcaaatg caaagttggg agctgttcat
     1261 ggttttgcag gtgtactggg aggaagctta aacgcaccac atggagcaat ttgcgcccgc
     1321 ctgttgcccg ttgtgatgtc gataaacgcg cgcgcattga gcgagcgcgc tccgcaaagc
     1381 gaggcttttg cgcggtgtga cgaggtggcg caaattttaa ctggcaaggc gaatgcccga
     1441 gcagctgacg gaattgcctg gttatatgat ttggtagcag gtttgggtac agctccgttg
     1501 tcatccttcg gacttcggag gaatgatttt cctggaatag tgcaaaaatc tgctaaagct
     1561 agcagcatga aagggaatcc ggtacaattg acacaggagg agatgagtga gattcttgag
     1621 ctgtcgctat aattactagt gtatgagtcg aatccgatct
//
LOCUS       DBNHOH010000319         1613 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_82466, whole genome shotgun sequence.
ACCESSION   DBNHOH010000319 DBNHOH010000000
VERSION     DBNHOH010000319.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_82466"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..>1613)
                     /locus_tag="AC16V7_05715"
     CDS             complement(93..>1613)
                     /locus_tag="AC16V7_05715"
                     /inference="COORDINATES: protein motif:HMM:NF017050.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HIR6256651.1"
                     /translation="TQAQTGREYSFPKTNWKFWEYLWAPALRCFGILQITAFRDMIVM
                     HGVGVGGGSLGYANVLMQPSDDLFEHPAWKHLADWKSILLPHYQTARHMLGATPNPCL
                     WESDQVLNEIATELGAENTFQPVEVSVFFGEHGQEGQEVPDPYFGGEGPARNACIHCG
                     ACMIGCRYNAKNTLDKNYLYFAEKWGAQVRAESEARDIRPLTDRQPDGARYEVIYMRT
                     TALGFKPRRSVRARNVVISAGTLGTLHLLFRCRDVTRSLPHISARLGDTVRTNSESLL
                     GAVSRDAEVDYSKGVSITSIFQPDAVTALEPVRYPAGSSLMRFLSSPLVEPGSSIPKR
                     FLRTALMILRYPLDFLRAYVLPGWAQRTTILLVMQTENNFTRMRLGRHIFTLFRKRLV
                     TQSNGAYKIPGSIEIGHNVTRRFAEKVNAAPAGTINEGLLNIPMTAHILGGYPFGRDD
                     QEGVIGLDFQVHNYPGLFIVDGSVVPANPGINPTLTITALSEYAMSQIPPISTKGR"
BASE COUNT          367 a          418 c          458 g          370 t
ORIGIN      
        1 tgtctctggc ccgcaggctg caaagaataa gacgatgaaa gttatgatta atatcagagg
       61 tagggatgat ttggataaaa ctgtcatttc ttttacctcc ctttagtgga aataggaggt
      121 atctggctca tagcatattc agacagtgcg gtgatggtca atgtagggtt gatgcctgga
      181 ttggcaggca caaccgatcc atccacgata aagagtccag ggtaattatg gacttggaag
      241 tccagaccaa tcacgccctc ctgatcatct cgtccaaagg ggtagccacc caaaatgtga
      301 gcggtcatgg ggatgttcag caaaccctca ttaattgtgc cagccggtgc agcgttaacc
      361 ttttccgcaa acctgcgtgt aacgttatgc ccgatctcaa ttgatcccgg gattttatac
      421 gctccattgc tttgggtgac caggcgtttt cggaaaagag tgaatatgtg gcgacccagg
      481 cgcattcggg taaagttgtt ctctgtctgc atcaccagca agatggtcgt gcgctgcgcc
      541 cagcctggta atacataggc acgcagaaaa tccagcggat accgcaatat cattaaggct
      601 gtgcgcaaga atcgcttggg tatactgctc cccggctcta ccagtgggct ggagagaaaa
      661 cgcatgagcg aggagcccgc cgggtagcgc accggctcca aagctgtcac tgcgtcgggt
      721 tggaaaattg aagttatgga gacgcccttg gaataatcca cctcggcgtc gcggctgaca
      781 gctcccaaca gggactccga gttggtgcgc acggtatccc caagtcttgc agaaatgtgg
      841 ggcaggctgc gagtgacgtc tcggcagcgg aaaagtaaat gcagagtgcc cagggtccca
      901 gcggagatga ccacgttgcg agcgcggaca gaccggcgcg gtttgaagcc caaggctgtt
      961 gtgcgcatgt agatcacttc gtagcgcgct ccatcgggtt gcctatccgt cagtgggcgt
     1021 atgtcacggg cttcggattc agcgcgcact tgtgctcccc atttttcagc aaaatagagg
     1081 tagttcttgt ccagagtgtt cttggcattg tagcgacaac caatcatgca cgcgccacag
     1141 tggatgcagg cattgcgcgc tggcccttcg ccaccgaagt aaggatcggg cacctcctgg
     1201 ccttcttgtc catgctctcc aaagaagacg ctcacctcta ccggctgaaa tgtattttcc
     1261 gcgcccagtt ccgtggcgat ctcattgagc acctggtctg attcccacag gcacgggtta
     1321 ggtgtcgcgc ctagcatgtg ccgggcggtt tggtagtgag gtaaaaggat ggatttccag
     1381 tcagccaggt gcttccaagc ggggtgttcg aagaggtcgt cggagggttg catcagcacg
     1441 ttggcgtaac ccaggctacc cccacccacc ccaacaccgt gcatcacaat catgtctcga
     1501 aaggcggtaa tttgcaagat gccaaagcag cgcaatgcag gcgcccatag atattcccag
     1561 aacttccagt tggtttttgg gaaggagtac tcgcggcctg tttgtgcctg cgt
//
LOCUS       DBNHOH010000320         3161 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_274528, whole genome shotgun sequence.
ACCESSION   DBNHOH010000320 DBNHOH010000000
VERSION     DBNHOH010000320.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_274528"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="AC16V7_05720"
     CDS             <1..228
                     /locus_tag="AC16V7_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256652.1"
                     /translation="VLSQEPIFICLTAYAIRASALSLYYSLQEMVAGFAGRISVGELA
                     LEEHSAGRMLSMAIFARWESVTILDAQRKVI"
     gene            373..1140
                     /locus_tag="AC16V7_05725"
     CDS             373..1140
                     /locus_tag="AC16V7_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HIR6256653.1"
                     /translation="MADVIEYKIFGDDLQLVEVELDPGEGVRAEVGTMTYMEQGIEMQ
                     TGTGGGIMKGLMRMFTGESFFITAFYNSGQNKSRAAFAAPYPGKIIPLDLGALGGRFL
                     CQKDSFLCAEKSTEIEVAFTKRIGAGLFGGEGFILQRLVGDGLVFVHAGGTIIEKDLA
                     PGDVLRVDTGCLVAFATTVDYDIQFIGGFKNVLFGGEGLFLAKLTGPGKVYLQSLPLS
                     RLADRINAAARGRQVDERKGVAGVGGDILGNILSGNS"
     gene            1460..2452
                     /locus_tag="AC16V7_05730"
     CDS             1460..2452
                     /locus_tag="AC16V7_05730"
                     /inference="COORDINATES: protein motif:HMM:NF024930.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0005085 - guanyl-nucleotide exchange
                     factor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RCC1 domain-containing protein"
                     /protein_id="HIR6256654.1"
                     /translation="MSNRFYRFLCLTICVGLIAHPGQAASAPKASNASDIAWEAGLDK
                     MSSVSAGLDHSCGIKLDGTVACWGSNSDGKTDPPAEVFSLISPGDDHKISASTFHNCG
                     IKQDGTVVCWGNNEFSQSTTPGGTFTQISAGGSHTCGVKADDTVACWGLDLDGQTSPP
                     EGEHFKQVSAGIEHTCGVKLDETLACWGNPFLPEIYDTPDGTFKQISAGGYHNCAVKK
                     SDNTLACWGAGTENTGIDPDYGQAMPPGDAFTQVSAGGYHTCGIRTNGTVACWGYNYY
                     NQATPPAGVFTQVSAGDFHTCGIRQDGTVTCWGDNFFGQAPRGVTPLFLPLVSR"
     gene            complement(2457..>3161)
                     /gene="mazG"
                     /locus_tag="AC16V7_05735"
     CDS             complement(2457..>3161)
                     /gene="mazG"
                     /locus_tag="AC16V7_05735"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HIR6256655.1"
                     /translation="PWDKEQTHQTLRSHLIEESYEVLAALDAGDPQAMREEFGDLLLQ
                     IVLHAQIASEFGEFTMSDVLESIHTKIVHRHPHVFGNLALKDVQGVLENWERLKEAER
                     NANGKAEVGLLDGVAKALPALVQAQEYQKRAGRVGFDWPNVQGVIDKVHEELREVKAA
                     RTADDQAAEIGDLLFAVVNLARWVDVDAESALREANARFRSRFAQIEAAARAQNRSIT
                     DLSLDEMEALWQAAKK"
BASE COUNT          712 a          866 c          892 g          691 t
ORIGIN      
        1 gtcctcagcc aagagcctat ctttatatgc ctgaccgcct acgccatccg cgcctcagcc
       61 ttgagcctgt attacagctt gcaggagatg gttgccgggt tcgctggacg gatcagcgtc
      121 ggtgagcttg ccctcgaaga gcacagtgca ggaaggatgc tttcgatggc gatattcgcc
      181 cgctgggaga gcgtgacgat cctggatgcc cagcgaaagg ttatctagga taaatttcgg
      241 ccacgcagtt actgcgccaa acaagcttgg caatagccgg gctcaaatat tagcaccagg
      301 tataataaat gcattgcgcc accgggctta ttaagacaaa ttcaatttca ccaaaaatat
      361 aggaggtcaa tcatggcaga cgtgatcgaa tataaaatat ttggcgatga cctgcaactg
      421 gtcgaagtag agctggatcc tggcgaaggc gtgcgcgccg aagtcggcac gatgacctat
      481 atggaacaag gcatcgagat gcagaccgga accgggggcg ggatcatgaa aggcctgatg
      541 cgcatgttca ccggagagag cttcttcatc actgcattct ataacagcgg gcagaataag
      601 agccgggcgg ccttcgcagc cccctaccca gggaagatta tcccgctcga cttgggcgct
      661 ctgggtggtc gcttcctgtg ccagaaagac tccttcctat gcgctgaaaa aagcaccgaa
      721 attgaagtgg ctttcacaaa acgtatcggt gcaggtctgt ttggcggcga aggcttcatc
      781 ctgcagcggc tcgtgggaga tggtctggtt tttgtccacg ctggcgggac gattatcgag
      841 aaagacctgg ctcccggcga tgtgctgcgc gtcgatactg gctgcctggt ggcttttgcc
      901 accacggtgg attacgatat ccagttcatc gggggtttta agaacgtgct tttcggcggt
      961 gaagggctgt ttctagccaa gctgaccggc cccggcaagg tctacctgca aagcctgccg
     1021 ctatctcgcc tggccgaccg catcaatgct gctgctcgcg gccgtcaggt tgatgagcgc
     1081 aagggtgttg cgggagtggg cggcgacatc ctgggcaata tcctcagcgg caactcatag
     1141 aatcgctttc ttcgcccaga agtccatcca taagggtttg catcgtatcc aatcaccccc
     1201 aagctccatc atgaagcctg ggggcgattg gtttcgcttt aggcaatgcc taccaagaat
     1261 tgcttgacga gatcaattaa ctgggtatac tgaggtacat taagtatagc tgcgcatcta
     1321 tcgtaagcgc gtatttcgct cagaggggaa gaacgcaggg agctggcaag aaaccacacg
     1381 cttcccatgc catgtcggcg gcatgacatc cccatctagt ttgtatgtat tacctgccct
     1441 atcaatgagg ctagcatcta tgagcaacag gttttatcgc tttttgtgtt taaccatctg
     1501 tgtgggcttg attgcacacc ctggtcaggc agcgagcgct cccaaggcga gcaacgcctc
     1561 agacatagca tgggaggcgg gattggacaa gatgagctca gtcagcgccg gccttgatca
     1621 cagctgtggg atcaagctgg atggtacggt agcctgctgg ggttcgaata gcgacggcaa
     1681 aaccgacccg cccgcagaag ttttcagcct aatcagccca ggcgatgacc acaaaatcag
     1741 cgcgagcact ttccataatt gcgggatcaa gcaggacggc actgtagttt gctgggggaa
     1801 taatgaattc agccaatcca caacgccagg gggcactttc acccagatca gcgcaggtgg
     1861 gagccatacc tgcggggtca aggcggatga tacggtggct tgttgggggt tggatttaga
     1921 cggccagaca tcgccacctg aaggagaaca ctttaaacag gtaagcgccg gcatcgaaca
     1981 tacctgtggt gttaagctgg acgaaacgtt agcctgctgg ggcaatccct tcctccctga
     2041 aatctatgac actccggacg gaaccttcaa acaaattagt gcaggtggct accataactg
     2101 cgcggtgaaa aagagcgaca atacgctggc ttgctgggga gctggcactg agaatactgg
     2161 catcgacccg gattacggcc aggctatgcc accaggagat gccttcaccc aggtcagcgc
     2221 cggtggctac catacctgcg ggatcaggac gaacgggaca gtagcctgct gggggtataa
     2281 ctactacaac caagccaccc cgcccgcagg tgtcttcacc caggtcagtg caggcgactt
     2341 ccacacctgc ggaatcaggc aggacgggac ggttacttgt tggggagata atttttttgg
     2401 tcaagccccc agaggggtca ctccgctatt cctgcctctg gtgagcaggt aagatttcat
     2461 tttttcgcgg cctgccacag ggcttccatc tcgtccaggc tcaggtcagt gatcgagcgg
     2521 ttttgggctc gtgccgcggc ttcaatttgg gcaaaacgac tgcggaagcg cgcgttagcc
     2581 tcgcgcaggg cgctctcagc atctacatca acccagcgag ccaggttgac caccgcaaag
     2641 agcaggtcgc caatctcagc tgcctggtcg tctgccgtgc gggcagcctt gacttccctg
     2701 agctcctcgt gcactttgtc aatcacgccc tggacatttg gccaatcgaa accgacccga
     2761 ccggctcgct tctggtattc ctgcgcttgc accagcgcgg gaagcgcctt tgcaacgcca
     2821 tccagcaagc caacctcagc tttgccattg gcattgcgct cagcttcctt caggcgctcc
     2881 cagttctcta acactccttg tacatctttc aaagccaggt tgccaaagac atgcgggtgg
     2941 cggtgcacga tctttgtatg aatgctttcg agcacgtccg acatggtgaa ctcgccgaat
     3001 tcgctggcaa tctgggcatg cagcacaatc tgaagcagca aatcgccaaa ctcttcgcgc
     3061 atagcctgtg gatcgcctgc atccagcgca gccaggacct cgtagctttc ctcaatcagg
     3121 tgggagcgaa gcgtttggtg ggtctgctcc ttgtcccaag g
//
LOCUS       DBNHOH010000321         3118 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1236501, whole genome shotgun sequence.
ACCESSION   DBNHOH010000321 DBNHOH010000000
VERSION     DBNHOH010000321.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1236501"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="AC16V7_05740"
     CDS             <1..514
                     /locus_tag="AC16V7_05740"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR6256656.1"
                     /translation="VTLALIITIIQVLRLSSGTYEEFVFAHLHHGNWETFYNQPWRMI
                     TSPFIHRDFLHYLGNLIWLLFFGYQIEKIYGGKYVLGVFFGALITGYFTHLTIMHEGI
                     IGISGGVCGLFGFSLVANRRTPWWKTFTKNPLYIIYLAGLIWFLIEDVANPEPNQVAH
                     INHLGVFHLS"
     gene            complement(520..1062)
                     /locus_tag="AC16V7_05745"
     CDS             complement(520..1062)
                     /locus_tag="AC16V7_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256657.1"
                     /translation="MFRETDERRLDWMKQHTLQLLSGKTKRLITEFRKLAKQPKTRKT
                     ARTQLLTTANYFERNLPFMDYPTYLARGYPIASGVIEGACRHLVKDRCELSGMRWSQC
                     GAENLLRLRSVAENNDWHDYHAYRRRQRHLRLYGSLPLHLPPTEMQSLVVAQPAPHML
                     VEQPTPLQHATAYARLPLAN"
     gene            complement(1132..2901)
                     /locus_tag="AC16V7_05750"
     CDS             complement(1132..2901)
                     /locus_tag="AC16V7_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256658.1"
                     /translation="MSKNEAPQLTHHAMLIAWGQFAQCLGLIEDLSAIPIKQKTVDHS
                     PQSKIQEFLVANLAGLAHLKEISIAAHPLAQDLAVAQAWGQSSWADYSGVSRTLAHLT
                     REETEQILKVLAKVSQPFIDKEAVLALRAEGRLTLDGDLSGRPVSNTSRTYPEVGFGH
                     MDDQVRLGYQAAMVCLQSPTYDRLWLSVTQHPGDTVSCTEAEPMIRRSEAVLGLRPTR
                     RVHLLRKRVADLEGRQISLKAVNEKASQSLASARQDLEEVSQQVQQWEHCVQETEASY
                     EKKERPVRPNSRLAQSRTKLACYQKRQKRREATLSQAEKRLTRQQKEWQAWQSEMDLL
                     QARLLQFEQDNQNNTVPIQAIFRMDAGFGTMENVALLIEMGYEVYTKPHGNWLLGRLK
                     NWVKAQSSWERVGKNAEMVAWSAKELSDFPYPLDLALERFQIGQQMQFGGLIHFGQDA
                     VAEHLTDWFERYNQRQTIEAGNKEEKQVFELHHLKVRSRSGILLQEQFVAFAANFVRW
                     ATLWLQEDCANAAEGWLDPVHPHVKDQVKVGARSLAWVSWYEQGCMLRFEDHSVFAGR
                     SLNIKKQWTFQLVLPFAKSYLFS"
BASE COUNT          729 a          779 c          737 g          873 t
ORIGIN      
        1 ggttactctc gctctcatta tcacaataat acaggtactc cgcctgtcaa gtggtacata
       61 cgaggagttt gtatttgccc atttacatca tggaaattgg gaaacattct acaaccaacc
      121 ctggcgcatg attaccagtc ccttcatcca tcgggatttt ctgcattatc ttggaaattt
      181 aatctggctt ctattttttg gttatcaaat tgaaaaaata tatgggggaa aatatgtatt
      241 gggagtattt tttggtgcct tgataactgg ttatttcacc catctcacta tcatgcatga
      301 aggaataata ggaatctcag gtggagtgtg tggtttgttt ggattttccc tagttgctaa
      361 ccgccgcacc ccctggtgga aaacctttac gaaaaacccg ctgtatatta tttatttggc
      421 agggttgatt tggtttttga tcgaggatgt tgcaaatcct gaaccaaatc aggttgctca
      481 tataaatcat ttgggtgtgt ttcatttgag ttgacaggtt caattcgcca ggggcaatct
      541 tgcataagca gtagcatgtt gcaaaggggt gggctgctcg acaagcatgt gcggagcggg
      601 ttgtgcgaca acaagggatt gcatttcagt tggcggcagg tggagtggga gcgagccata
      661 caaacgcaga tggcgttgcc gtctgcggta agcatgatag tcgtgccagt cgttgttttc
      721 ggcgacggag cgcaaacgca agaggttttc agcgccgcat tgcgaccagc gcatgccaga
      781 caattcacag cggtctttca ccaaatggcg gcaagcgccc tcgatcacgc cggaagcaat
      841 cgggtagccg cgggccaggt aagtcggata gtccatgaaa ggcaggttgc gttcaaaata
      901 gttggctgtg gtcagcagct gggtgcgtgc ggttttgcgg gtcttgggtt gtttcgccaa
      961 cttgcgaaat tcggtgatca gccgcttggt ttttcctgaa agcagttgca gggtatgttg
     1021 tttcatccag tccaggcggc gctcgtcggt ctctctgaac aacgcattgg caaccttcca
     1081 gagatattca ttagcgtaag tattgtgcaa ccagtttgaa aagtcggaaa atcacgaaaa
     1141 aaggtaactt ttagcaaacg gaaggaccaa ttgaaaagtc cattgttttt tgatatttag
     1201 tgatcgtcca gcaaacacgc tgtgatcttc gaaccttaac atacaacctt gctcatacca
     1261 gctgacccag gctaacgacc gggcgcccac cttgacctga tccttgacat gcggatgaac
     1321 aggatcaagc caaccctcag cagcattcgc acaatcttct tgtaaccaga gagtcgccca
     1381 acgcacaaag ttagccgcaa aagccacaaa ctgttcctgc aataaaatgc ctgaacggga
     1441 acgaaccttc agatggtgca gttcaaagac ttgcttctcc tctttgttcc cggcttcaat
     1501 ggtttgcctc tggttatatc tttcaaacca gtcagtcaaa tgctcagcca ctgcatcctg
     1561 tccgaaatga atgagcccac cgaattgcat ctgctgtcca atctgaaagc gctccaaggc
     1621 cagatcgagc ggatatggaa aatctgacag ctccttcgcc gaccaagcca ccatctcagc
     1681 attcttccca acgcgttccc aagaagattg agccttgacc caatttttca aacgaccgag
     1741 cagccagttt ccatgaggct tggtatacac ttcgtagccc atttctatca acaaagctac
     1801 attttccatt gtgccaaagc cagcgtccat tcggaatatt gcttgaattg gcactgtgtt
     1861 gttttggtta tcctgctcaa actgcagcaa acgcgcttgc aaaagatcca tttcactttg
     1921 ccacgcttgc cattcttttt gctggcgggt cagtcttttc tctgcttgac ttagggttgc
     1981 ctctctccgt ttttgccgct tttggtagca agccagcttc gtccgagatt gtgctaatcg
     2041 actgtttggt cggacgggtc gctctttctt ctcgtaactg gcttccgtct cttgaacaca
     2101 gtgttcccac tgttgaactt gttggctcac ttcttcaaga tcctgacggg cacttgccag
     2161 ggattggctg gctttttcgt tgaccgcttt caggctgatt tgcctgcctt ccagatccgc
     2221 cacccgcttt cggagcagat gcacccgtcg ggtcgggcgc aagcccaaga ccgcttctga
     2281 cctgcggatc atcggctctg cttcggtgca agaaaccgtg tcaccaggat gctgggtcac
     2341 tgagagccaa agtctgtcat aggtcgggga ctgcaggcac accatcgctg cctgatatcc
     2401 cagacgcact tgatcatcca tgtgaccaaa accaacttct ggatacgtgc gactggtgtt
     2461 ggatactggt ctgccggata gatcaccatc cagagtcagc ctgccttctg ctctcagtgc
     2521 cagaaccgct tctttatcaa taaagggttg gctgaccttt gccaaaactt tcaagatctg
     2581 ctctgtttct tcccgcgtca aatgtgccaa ggtgcggctg acgccactgt agtccgccca
     2641 actgctctga ccccaggctt gggcgactgc cagatcttga gcaagcggat gcgcggctat
     2701 actgatctct ttcaagtggg ccaaccctgc caggttcgct accagaaatt cctggatctt
     2761 ggactgcggc gaatgatcta ccgtcttttg tttgatgggg attgccgaga ggtcttctat
     2821 taatcccaga cattgggcaa actgccccca ggcaatcaac atggcatgat gggttagctg
     2881 cggtgcttca tttttactca tggctcttct ccatttatag ttgccataag cttactcgac
     2941 cagagttcgt aggttgtcaa tgttctattt ttttacgttt tttcgacttt tcgattctgt
     3001 tgcacaatac ttacgttaga tagaaacggt gatgtattat tacaacttta agacaaatcc
     3061 accagaaatc ccatatgcaa aaaccataac tgattttgta cgccattcat gtggatca
//
LOCUS       DBNHOH010000322         1547 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_851701, whole genome shotgun sequence.
ACCESSION   DBNHOH010000322 DBNHOH010000000
VERSION     DBNHOH010000322.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_851701"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="AC16V7_05755"
     CDS             <1..719
                     /locus_tag="AC16V7_05755"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HIR6256659.1"
                     /translation="GIEVYLVTATRGERGWFGTREDIPGKEALGRIREAELRCAAQTL
                     GIKQVEFLDYIDGDLDKADPTEAVGKIVESIRRVRPDVVITFPPDGAYGHPDHIAICQ
                     FTTAALVSAADPTYPVKNQIQPHRVSKLYYTADNQELFEIYTQIFGDLRMTIDGKERQ
                     AVAWSDWAITTHIDGEKYWPSTWQAVQCHASQIAGLGDRERITEEQHRRLWSERTYYR
                     AYSLVNGGRKIETDLFAGLR"
     gene            726..1517
                     /locus_tag="AC16V7_05760"
     CDS             726..1517
                     /locus_tag="AC16V7_05760"
                     /inference="COORDINATES: protein motif:HMM:NF015534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HIR6256660.1"
                     /translation="MKSMIALIGGEEFADGFEDVHANLLELSFSNRSQKNGKPVQVVF
                     LPTCATEDGADTITYWCDEATKRLEAVGAKVKPLRIVDRPSGDDPENARLIIEADWIY
                     IGGGYPHVGMRILRGTRAQQALEAAWQRGALIAGASAGAMMLGAQSWVITPELDAAVT
                     KLFSQGAGISEWDLPLPPALECLGFVPRSMCWPHANQLFSLRWLKSGLLPPHHFVIGI
                     DEQTAFVQTANGGWKVLGRGKVTLVDDQFQVKELNPGSQIENLIM"
BASE COUNT          389 a          399 c          415 g          344 t
ORIGIN      
        1 aaggtatcga ggtttatctg gtaacagcca cgcgcggcga acggggttgg tttggcacca
       61 gagaggatat tcccggtaaa gaagcgttag ggcgaatccg tgaagccgag cttcgctgcg
      121 ctgcccaaac gcttggcata aagcaggtgg aatttctgga ttatatcgat ggagatcttg
      181 acaaggctga tccaacagaa gccgttggta agatcgtcga aagcatccgt cgagttcgcc
      241 ctgacgttgt aattaccttc ccacccgatg gagcttacgg gcatcccgac catatcgcta
      301 tctgccaatt cacaactgct gcactcgtca gcgcggctga ccctacctac cctgtaaaaa
      361 atcaaatcca accccaccgc gtctcaaagc tgtattacac ggcggacaac caggagttgt
      421 ttgaaatcta tactcagata tttggcgatc tgcgcatgac aatcgacggt aaagagcgac
      481 aggcagtagc ctggtctgat tgggctatca ccactcacat cgatggggag aaatattggc
      541 cttctacctg gcaagcagtc caatgccacg catcccaaat tgcagggctt ggtgaccggg
      601 aacgaataac cgaagaacag cacaggcgct tgtggagcga gcgcacttat taccgcgctt
      661 atagcttggt aaatggcggg cgaaaaatcg agaccgatct atttgctgga ttgcgctaaa
      721 tagtcatgaa atcaatgatt gccttaattg gaggcgaaga atttgcagat ggcttcgagg
      781 atgttcacgc taacctgcta gaactgtctt tctccaaccg tagccagaag aatgggaagc
      841 cagttcaggt agtatttctg ccaacgtgtg cgaccgagga cggtgcagat acaattacct
      901 actggtgcga cgaagcaacc aagcgtctcg aagcggtggg agccaaggtt aaacctttgc
      961 gcattgtcga tcgacccagc ggcgacgatc ctgagaacgc acgcctgatc atcgaggctg
     1021 attggattta catcggtggt ggatatccac acgtcggcat gcgcatcctg cgtggcaccc
     1081 gagcgcagca agcgcttgaa gctgcttggc agcgcggcgc gttgatcgct ggcgccagcg
     1141 ccggcgccat gatgctgggt gctcaatcct gggtgatcac cccagagctt gacgcggctg
     1201 tcaccaagtt attctctcaa ggagctggga tttccgagtg ggatttaccc ctgccacctg
     1261 cactggaatg cttgggtttt gtgccccgca gcatgtgctg gccccatgca aaccaattat
     1321 tctcccttcg ctggctaaaa agtggactgc tcccgccgca tcattttgtc atcgggatcg
     1381 acgaacagac cgcctttgtg caaacagcca atggtggatg gaaagtcctt gggcggggca
     1441 aagtcaccct cgtggatgac caatttcagg taaaagagct gaatccagga tctcagatag
     1501 aaaaccttat tatgtaaaca aatctggatc acaaaccgaa attagtc
//
LOCUS       DBNHOH010000323         6953 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1107748, whole genome shotgun sequence.
ACCESSION   DBNHOH010000323 DBNHOH010000000
VERSION     DBNHOH010000323.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6953)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6953)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6953
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1107748"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="AC16V7_05765"
     CDS             complement(<1..1104)
                     /locus_tag="AC16V7_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR6256661.1"
                     /translation="MMTELPAYLPAEVEPKWQARWEAEGQYHANVDPSRPKHYALTML
                     PYPSGDLHIGHWYAMTPSDARARFMRMRGYNVMFPMGFDSFGLPAENAAINRGVHPKE
                     WTYANIERMRTQLRSMGAMFDWRREAVSSDPEYYRWSQWFFTQLHKNGLAYRKMSPVD
                     WCPNCNTTLAREQVWGEDRHCERCGTPVIKKDLEQWFFRTTNYAEELLDFSHLDWPER
                     VQLLQTNWIGRSEGAEVHFIVDDPRLSVLESQLTVFTTRPDTLWGATFMVLAPEHPLV
                     AKITTSDQKAEVDAYVQQASRMTDIQRESADKEKTGVFTGAYAINPVNDERIPIWIAD
                     YVLMTYGTGAIMAVPAHDQRDFEFARKFRLEVRV"
     gene            complement(1494..1566)
                     /locus_tag="AC16V7_05770"
     tRNA            complement(1494..1566)
                     /locus_tag="AC16V7_05770"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1531..1533),aa:Ala,seq:ggc)
     gene            complement(1623..1695)
                     /locus_tag="AC16V7_05775"
     tRNA            complement(1623..1695)
                     /locus_tag="AC16V7_05775"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1660..1662),aa:Ala,seq:cgc)
     gene            complement(1750..2418)
                     /locus_tag="AC16V7_05780"
     CDS             complement(1750..2418)
                     /locus_tag="AC16V7_05780"
                     /inference="COORDINATES: protein motif:HMM:NF014269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR6256662.1"
                     /translation="MDVNVMDLPLFPLNVVLFPGMPMQLHIFEERYKEMVQVCLRSNR
                     IFGVALIRDGEEASGPLPEPYKVGCMASIVDIQPLEEGRMNLTVVGRERIQILSLDRE
                     SAPYLIGRVETFPLKDGGEESLHQVAKQLRPKIEQYLQLLVRLSDRQREPDPLPDDPK
                     LVAYLAAILLQIPLPEKQTFLEVETIIEFYDLLGGIIDRELALLRAMLSESSRDGIGV
                     FSRN"
     gene            2624..3757
                     /locus_tag="AC16V7_05785"
     CDS             2624..3757
                     /locus_tag="AC16V7_05785"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR6256663.1"
                     /translation="MPTIEFKTPYLRRQNRLRQAMQKAGIDALALNPGPSLTYLTGLY
                     FHLMERPVVVLFTTKRPPTFVLPELEAGKTSNLTFDLITSPFGEDPQTWPGVFKEAVK
                     GLEISKNARVGIEPRRLRVLELRLLESASPGSSFTSAEDVIAELRMHKDPEEISAMRQ
                     AVEFAQNALQATLPSIQIGMSEKDIAAELTLQLLRGGSESQLPFPPIVASGPNSANPH
                     SVPSDRRLQPSDLLVIDWGASHQGYFSDLTRTFAVAEILPEFDQVAKVVAAANEAGRL
                     AARPGVPASTVDIAARNVIERSGFGEYFIHRTGHGLGMEEHEEPYIRGDNDQPLAEGM
                     TFTVEPGIYLPNRGGVRIEDDIVITAQGSESLSDMPRDLRIVG"
     gene            3770..4927
                     /locus_tag="AC16V7_05790"
     CDS             3770..4927
                     /locus_tag="AC16V7_05790"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6256664.1"
                     /translation="MGRDLILMAFALTTWGIGEGAFVGFQTLYLQELGADPLRIGTII
                     GGFGLAASLAHIPAGYLSDRLGRRPLLWVAWIIGAIAAWIMATANSLAVFVAGMMLYG
                     ITAFVISPLNSYITAARGRWSVGRVLTLISATYNTGAILGPLLGGMIGERYGYQKIFL
                     FSACVFILSTLIILFIRPQPVDTPEPEEKGKNLIRNRRYLYFVFAYSLATFAMYLPQP
                     LSPNFLQNHRGLGPAQIGQLYSISSVGIVILNLLLGQLNARIGYLIGQVGVAIFALLL
                     WRGDQFLIYGLAFFILGGFRLARSLATAHIRSIVSQSNMGLAYGLAETLTAISLLFAP
                     LLAGYLYDRDPTQIYMFSLGIIGVSVLVNSVLSAGPRTPTKIEEGYSTIET"
     gene            complement(5021..>6953)
                     /locus_tag="AC16V7_05795"
     CDS             complement(5021..>6953)
                     /locus_tag="AC16V7_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR6256665.1"
                     /translation="ISDFEEEEQRRNLSLSTAIIPIEYNDYKINFLDTPGYTDFVGEV
                     ISALRVSDGALILVDSVAGVEVGTEIAWNYCDTFNLPRFVVINKMNRDNANFRKAVES
                     VQNISDSRLISVQLPWGEKLDFNGVIDLLTMKAYAGDGKNPQAIPAELIEEAEAARME
                     LIEAAAEGEDALLEKYLEGEELTADEIKRGLRKVVRSGSFVPVLVAAGSAEIGLGPLL
                     DAVVSLMPSPKDLPPVTASGKGGDEEITPVDNGPLAAYVWKTTADPFVGKITYFRIYS
                     GMITSDSRVWNETKSSEERLGTLHLLRGKEQIAVKVIHAGDIATVPKLTTTSTGDTLT
                     DRGHPLTLAVPEYPNALYQVAVFPKTQADSAKISPTLTRLCEEDMTLSWRQEPSTNQT
                     ILQGMGDQHIDVAIRKSETKFQTSLLMELPKVPYQETITKQGQAMYRHKKQTGGAGQF
                     GEVHMRVEPLPDEDYAFSNDVFGGAISSSYMPAIEKGIKAVMKEGAIAGYPIKNVKVS
                     VIDGKEHPVDSKPVAFEIAGREAFKLSVKDASPVLLEPIMNVRVTVPETNMGDVLGDL
                     NTRRARVQGMDTDKGRSVVTAQVPLAEMQRYTTDLRSITGGRGVFTMEFSHYEVVPSH
                     LQQDIINVRQKELQTAKEE"
BASE COUNT         1634 a         1890 c         1686 g         1743 t
ORIGIN      
        1 aacgcgcacc tccaacctga atttacgagc aaactcgaag tcacgctggt catgcgccgg
       61 cactgccatg atggcacctg tcccataggt catgagtacg taatcggcaa tccagatggg
      121 gatacgctcg tcattcacgg ggttgatagc gtaagcaccg gtaaatacac cggttttttc
      181 cttatcagcg gactcgcgct ggatatctgt catgcgcgat gcctgctgca catatgcgtc
      241 cacctcagct ttttgatccg aggtggtgat tttagcgacc agtggatgct cgggcgctaa
      301 taccataaat gtagctcccc acaacgtgtc ggggcgagtg gtaaaaacgg ttagttgcga
      361 ttcgagaacc gatagccgag ggtcatcgac aataaaatgg acttccgcgc cttccgacct
      421 tccaatccag ttggtctgta gcaattgcac ccgttctggc caatccaaat gggagaaatc
      481 cagaagctct tcagcgtagt ttgtggtccg aaagaaccac tgctccaggt ctttcttaat
      541 cactggggta ccgcagcgtt cgcagtgacg atcttctccc caaacctgct cgcgtgccaa
      601 tgtcgtgttg caattggggc accagtccac cggcgacatc ttgcggtagg ctaaaccgtt
      661 cttatgtaat tgggtgaaga accactgtga ccaacggtaa tattccggat ccgaggaaac
      721 cgcctcacga cgccaatcga acattgcacc catgcttcgc aattgagtgc gcatgcgctc
      781 gatatttgca taggtccatt ctttgggatg cactccacga ttgattgctg cattctctgc
      841 aggcaaacca aatgaatcga atcccattgg gaacatcaca ttgtagccac gcatacgcat
      901 aaaacgggcg cgcgcatccg aaggagtcat ggcataccag tgaccgatat gcagatcacc
      961 cgaaggatag ggtagcatgg tcaaggcata gtgcttgggg cgtgaagggt caacattggc
     1021 atggtattgg ccttcggctt cccagcgagc ttgccatttt ggttcaactt ccgcaggtaa
     1081 gtatgcaggt aattctgtca tcatttttac tctctgatat ttcctgtttg attcaatatt
     1141 cgggttagcc atactctcga ataaaaaaag cccttcgccc gaatacaggg acgaaggtgc
     1201 tccgcggtac caccctgctt gtcttccact gtttcattaa gacaacctac aggtcaataa
     1261 agctcatttc gaatagcaat ccgataacct gtaagcagtg gaagaccgct ttgatgcacg
     1321 ataacgggcg cacccgccac cgactactct tagttcaccg gcggagtctg cctcgtgggc
     1381 gacaggcgag ttcagcccaa ctcaaaatgt gctctacaaa caccctgccg ggcttccacc
     1441 tcgctctccc ggctcgctgg tagatggcct actactcctg ctagactttt ctgtggaccc
     1501 agagggattc gaaccctcga ccttctcaat gccattgaga cgcgctccca actgcgctat
     1561 gggccctgga atgttaactc agcgacggaa ttttaatcgc aaaaacccgt ttcgtcaaca
     1621 aatggacctg gcgggattcg aacccgcgac ctcttcagtg cgattgaagc gcgctcccaa
     1681 ctgcgctaca ggccctccat gcagagtggc atgattctat ctgagtgcag gattgctgtc
     1741 aagctggagc taatttcggg agaagacgcc gattccatct cggctactct cggataacat
     1801 ggcgcgcagc aacgccagct ccctatcgat aataccaccc agaagatcgt aaaattcaat
     1861 tattgtttcc acttctaaaa atgtctgctt ttcgggcagg gggatttgca ataaaattgc
     1921 tgccagatag gcaaccagct tggggtcatc tggcaacggg tcaggctctc gttgacgatc
     1981 gcttaaacgc accaagagtt gtaagtattg ctcgatcttt gggcgcagtt gctttgcaac
     2041 ttgatgcaaa ctttcctcac caccatcctt aagtggaaag gtttcaaccc taccaatcaa
     2101 atatggtgca gattcgcggt caagcgaaag gatttggatg cgctcgcgtc caacgaccgt
     2161 caagttcatg cgtccttctt ccaagggttg gatatctaca atgctagcca tacaacccac
     2221 cttatacggc tcgggtaatg gacctgatgc ctcctcccca tcacggatga gcgctacacc
     2281 aaatatgcga tttgatcgca agcagacttg caccatctcc ttatatcgtt cctcaaagat
     2341 gtgcaattgc ataggcatgc ctggaaataa caccacattc aaggggaaca aaggcaagtc
     2401 catcacgttt acatccataa tcaatacacc tgcagcaatt tatcttaatt gacagagatc
     2461 agcagggcaa ttgcatatgc cctgaggggc atgcgcaatc ccgtaaaaag agagaatggg
     2521 gtgggaaatc gcccaaatgc gattgcccta gatcaggcgc taaagcgttt ttatgctatg
     2581 atagacctat tctaacatac cccctatccc tattgagatg catatgccca cgatagaatt
     2641 taagaccccc tacctgcgcc ggcagaaccg gctgcgtcaa gccatgcaga aggctggtat
     2701 tgatgcacta gctctcaatc caggtccaag tctgacatac ctgaccgggt tgtattttca
     2761 cctaatggaa cgtccggtgg tagtgttgtt cacaaccaag agaccgccaa cgttcgtgct
     2821 tcccgaacta gaagcgggca aaacctccaa cctgacattt gacttaatta ccagcccatt
     2881 tggcgaagat ccgcaaacat ggcctggtgt gtttaaggag gctgtaaaag gcttagagat
     2941 cagtaaaaac gctcgcgttg gtatagaacc ccgcaggctg agagtgttgg agctgcgtct
     3001 tctagaaagc gcttcgccgg gatcttcatt cacttcagca gaggacgtta tcgctgaatt
     3061 gaggatgcac aaagaccctg aagaaatttc ggctatgcgc caggcagtgg aattcgctca
     3121 aaacgcttta caagctaccc tgccctctat acagataggc atgtctgaaa aggatatagc
     3181 agccgaattg actctccaat tgcttcgggg cggctccgaa tcccagcttc cattcccccc
     3241 catcgtagcc tcgggtccca acagcgccaa cccacactcc gtccccagcg atcgccgtct
     3301 gcaacctagt gatctactgg taatcgactg gggggcttct catcaaggct acttctccga
     3361 tttaacgcgc acattcgccg tcgctgaaat cctgcctgaa ttcgatcaag tagcgaaggt
     3421 tgttgctgcc gccaatgagg caggtcgtct tgctgctcgc ccaggtgtac ctgcaagcac
     3481 agtcgacatt gctgcccgaa acgtgatcga acgatcgggt tttggagaat atttcatcca
     3541 tcgcactgga cacgggttgg gtatggaaga acacgaagag ccatatattc gtggtgataa
     3601 tgaccagcct ttagcagaag gcatgacctt cactgtggaa ccgggcattt acctgccgaa
     3661 tcgaggcggc gttcgcatcg aggacgacat tgttattacc gcccagggaa gtgagagtct
     3721 aagcgatatg ccgcgcgatt tacgaatagt gggataagca acctgaatta tgggccgaga
     3781 tcttattttg atggcctttg ccctcaccac ctgggggatt ggagaagggg cgtttgtcgg
     3841 tttccagacg ctctatctac aggaattggg agcggacccg cttcgcatcg gcacgatcat
     3901 cggcggtttt ggcttggcag ccagccttgc ccatattcct gcgggctact tatccgatcg
     3961 actaggtcgc cgtcccttac tgtgggtagc ctggattatt ggcgcgatcg cagcctggat
     4021 aatggcaacc gcaaactcgc tggcggtgtt tgtcgctggt atgatgctct atggtattac
     4081 cgcgtttgtt atctccccat tgaacagtta tattaccgcg gcacgcggca ggtggtcagt
     4141 ggggcgcgtc ctgaccttaa tttctgccac ctataatacg ggcgcaattc ttgggcctct
     4201 cttgggtggg atgatcggcg agcgctatgg ttatcaaaaa atatttctct tttcagcctg
     4261 cgtctttatc ctttcaacct tgatcatcct cttcattcgt cctcagcccg tcgacacccc
     4321 ggagcccgag gaaaaaggca aaaacctaat cagaaacagg cgctatcttt actttgtttt
     4381 tgcctattca ctggcgactt tcgccatgta tctaccccag cctctctcgc ccaacttcct
     4441 acaaaaccat cgcggtcttg gtcctgcgca aatagggcag ctatactcca tcagcagcgt
     4501 ggggattgtc atactcaatt tgctattggg acaattgaac gctcgaattg ggtatctcat
     4561 cggacaagtt ggcgtggcga tatttgcttt gctcctctgg cgaggggacc agtttctcat
     4621 ttatggccta gcttttttta tcctaggcgg attccgtctg gcgcgttcat tagcaaccgc
     4681 ccacatccgc tccattgtca gccaatccaa tatgggcttg gcctacggac tagctgagac
     4741 acttactgcc atctctttgc tcttcgctcc acttctagct ggctatcttt atgaccgcga
     4801 tccaacgcag atttatatgt tcagcttggg gatcatcggc gtctcggtgc tggttaactc
     4861 cgtcctgagc gctggacctc gaacccctac gaagatcgaa gagggatatt caacaattga
     4921 aacttgatca ttagaccgct taaaaggatt tcggggctca ggtgcagccc cgaaatttta
     4981 taatcaaatg ctatcgccaa aaatctcgct aaacaggcga ttactcttcc ttagcggttt
     5041 gcagttcttt ctgtcggaca ttgatgatat cttgctgcag gtgcgaaggg actacctcat
     5101 agtgggaaaa ttccatcgta aagactccgc gcccgccggt tatcgagcgt agatcggtag
     5161 tgtagcgctg catctccgcc aaaggcactt gcgccgtcac gacagagcgc cctttatcgg
     5221 tatccatgcc ctggacacgc gccctacgcg tgttcagatc acctagaaca tcacccatat
     5281 tcgtttccgg tactgtgacg cgcacattca tgattggctc cagcagcact ggtgaagcgt
     5341 ctttgaccga cagcttgaag gcttcccgac ctgcaatctc gaacgccact ggctttgagt
     5401 ccaccgggtg ttctttccca tcaattacag aaaccttcac attcttgatg ggatatccgg
     5461 caatggcgcc ctctttcatc actgctttaa tgcctttttc aatcgcaggc atatagctgc
     5521 ttgaaatggc acccccgaag acatcattgg aaaaagcata atcctcatca ggcaaaggct
     5581 caacgcgcat gtgcacctcg ccaaactggc cagcgccccc ggtctgtttt ttatggcggt
     5641 acatggcctg tccttgcttt gtgatcgttt cctggtaagg gaccttgggc aattccatca
     5701 gcaagcttgt ctgaaacttt gtctcagatt tgcggatggc cacatcaata tgttggtcac
     5761 ccatgccttg caggatagtc tggttggtgc tgggctcctg gcgccaggaa agtgtcatgt
     5821 cctcctcgca caagcgggtt aatgttgggc tgatcttcgc agagtcagct tgtgtcttgg
     5881 ggaagacagc cacctggtaa agcgcattgg ggtattcggg tacagcaagg gttaatggat
     5941 gccctcgatc agtgagcgta tcaccagtag aggtggtcgt tagcttaggc actgtagcaa
     6001 tatcgcccgc gtggataacc ttgactgcaa tttgctcttt gccgcgtaat aaatgcagcg
     6061 tgcccagcct ttcctcgctg gatttggttt cgttccagac ccgcgaatct gaagtgatca
     6121 tgcctgaata tattcggaag taggttatct tgccaacgaa gggatctgcg gtagtcttcc
     6181 agacataggc tgccagcggg ccgttatcaa ctggggtgat ctcttcgtca cccccttttc
     6241 cgctggctgt cacaggtggg aggtccttcg gggaaggcat caaagagacg acagcatcga
     6301 gcaacggtcc cagaccaatc tccgccgatc cagcggcaac gagaaccggg acaaaactgc
     6361 ccgagcgaac caccttgcgc agaccgcgct taatctcgtc agcggttagc tcctcgccct
     6421 ctaaatattt ttcgagcagg gcatcttctc cttctgctgc agcttcaatc aactccatgc
     6481 gcgccgcttc cgcctcttca ataagctccg ccgggattgc ctgaggattt ttgccatccc
     6541 cagcataggc tttcatggtc aataagtcga tcacaccatt gaaatccagt ttttcgcccc
     6601 aaggtaattg caccgatatc aagcgtgaat cactgatatt ctgcaccgac tcgaccgcct
     6661 tgcgaaagtt ggcattgtcg cggttcatct tattaatcac gacaaagcgc ggtaggttga
     6721 atgtatcaca ataattccag gcgatctccg ttcctacctc taccccggca accgagtcaa
     6781 ccaggatcag cgctccatcg gagacgcgta gcgccgagat aacctcccca acaaagtcgg
     6841 tgtagccagg tgtatccaaa aaattaattt tatagtcgtt atactcgata gggatgatgg
     6901 ctgtggacag tgaaagattg cgccgttgct cttcctcctc aaaatccgag atg
//
LOCUS       DBNHOH010000324         3633 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_18633, whole genome shotgun sequence.
ACCESSION   DBNHOH010000324 DBNHOH010000000
VERSION     DBNHOH010000324.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_18633"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2520)
                     /locus_tag="AC16V7_05800"
     CDS             complement(<1..2520)
                     /locus_tag="AC16V7_05800"
                     /inference="COORDINATES: protein motif:HMM:NF013957.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HIR6256666.1"
                     /translation="MPRNIKSFLSQYRIPALVIFVLLVSAGVYLIVRGWPRADSGGQQ
                     IPVDTGISTGQGGISEVETGEPDVSIQLSEGQAQPQTAVPVPQATGEPLTEDEIQQIL
                     SRLPALAMQPPDQVDFRLAQEPIPPPRPGETISEQFPPPPDVQPVEVEAGPLEVLRFA
                     PEGEIPIAPFVNITFNQPMVPLATLADLAAEQVPVRMEPALPGTWRWLGTKTLTFQYD
                     SELIDRLPKATLYRVTVPAGTQSAIGGRLAQAVEWTFSTPPPKMISTYPYDVPQPLEP
                     LFFIAFDQRVNPDAVLETIQVSAGGQPVSLDLVSEEGIQADKQVSRLVKNTPQGRWLA
                     FKAKAPLPADTSITVTVGPGTPSAEGPLVTKDTQSYSFRTYAPLRIDEHGCSWYSDQC
                     LPLTPLFIRFNNPIDAGAYEESMLRIDPELPGASVNIFGNTINIQGATKGQTTYTVYL
                     DGDIQDIFGQKLGQDARLTFRIGPAEPLLVGPESIFVTLDPVTSKPTFSVYTINYTKL
                     DVKIYAVQPSDWPAFKSYLREYQRTDNPPKPPGRLALDKTIQVEAPADTLNEVGIELN
                     QVMDGKFGHFIVIVQPPRGLFQEDQRWRAVQAWVQITQIGLDAFADHSQMVVWTTALK
                     DGTPLPGVTIQPGAGGPEIVTGEDGTARLAVPEGAAYLEASQGADHAMLPRSIYFWDD
                     DTWHRSFPSDQLRWYVFDDRQMYRPGEELHVKGWLRRIGAKQDGDVGLVGSAVSAIDY
                     RITDPQGNDLGSGQAEVNALGGFDFVFALPQAVNLGYAQLYLQARGPLDSLNNTQYYH
                     GFQIQEFRRPEFEVNARNETPGPYFAGGQAVVAVEAKYYAGG"
     gene            complement(2858..3070)
                     /locus_tag="AC16V7_05805"
     CDS             complement(2858..3070)
                     /locus_tag="AC16V7_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR6256667.1"
                     /translation="MADRIIGTVKWFNGSKGYGFISREDGPDVFVHFSAIMADGYRNL
                     MEGQKVEFTIENGPKGLQAANVLPVS"
     gene            complement(3267..3479)
                     /locus_tag="AC16V7_05810"
     CDS             complement(3267..3479)
                     /locus_tag="AC16V7_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256668.1"
                     /translation="MKEPGNNPLPSILVVIILLGLIYALYVMMGGDWLAFTKLTSSGE
                     RIVDGITGSMRALGDSLGRTFSSILR"
BASE COUNT          826 a         1012 c          961 g          834 t
ORIGIN      
        1 accaccggcg taatacttgg cctcgacggc aaccacagcc tgcccaccgg caaagtacgg
       61 tccgggagtc tcgttacgtg cattcacttc gaactcgggc ctgcgaaact cctggatctg
      121 gaaaccatga taatactgag tgttattcaa gctatccaac ggacctcggg cctgcagata
      181 gagctgcgca tatcccaggt ttacagcttg cggcagggca aagacaaagt caaagccacc
      241 caaggcattg acctcagcct gaccgctgcc caggtcgttg ccctgagggt cggtgatgcg
      301 gtagtcaatg gcgctcaccg cgctcccaac cagacccaca tcgccatcct gcttggcgcc
      361 gatgcgcctc aaccacccct tgacatgcaa ctcctcgccg gggcggtaca tctggcgatc
      421 atcgaacaca taccagcgca gctggtcgct agggaagctt ctatgccagg tatcatcatc
      481 ccagaaataa attgaacgcg gcagcatggc atgatcggca ccctggctag cctccagata
      541 ggctgcccct tcgggtacag ccaagcgggc ggtgccatct tcacccgtga cgatttctgg
      601 ccctcccgct ccgggctgga tagtcacgcc gggtagtggg gtgccgtcct tcagggctgt
      661 tgtccatacc accatctggc tgtgatcagc gaaagcgtcc agcccgattt gggtgatctg
      721 cacccaagct tgcacagccc gccagcgttg gtcctcttgg aagaggcccc tgggcggctg
      781 tacaatgaca ataaaatggc cgaatttgcc gtccatgacc tggttcaact caatgcccac
      841 ctcattcagg gtatcagccg gcgcctccac ctggatcgtt ttatccaacg ccaggcggcc
      901 cggcggcttc ggagggttgt ctgtgcgctg gtattcgcgc agataggact tgaaagctgg
      961 ccaatcggac ggttggacag cataaatttt tacatccaat ttggtgtagt taatcgtgta
     1021 caccgagaag gttggcttgc tggtgacggg atcgagagtg acaaatatcg attccggccc
     1081 caccagcaag ggttctgcag gaccaatgcg gaaggtcaag cgggcatctt gccccaactt
     1141 ttgaccaaag atatcctgta tatccccgtc taaatagacc gtgtaagtgg tctgcccctt
     1201 ggtagccccc tggatattga tcgtgttgcc aaagatattc accgaagcgc cgggcaattc
     1261 cggatcgatg cgcagcatgc tctcttcata agcgcccgca tcaatgggat tattgaagcg
     1321 gatgaacaaa ggcgtcagag gcaggcattg gtcactatac caggaacagc cgtgttcatc
     1381 gatgcgcagc ggggcatagg tgcgaaagct ataagactgt gtgtctttag taaccagcgg
     1441 gccttcggcg gagggggtgc ctggaccaac cgtcaccgtg attgacgtgt ctgcaggcaa
     1501 aggcgctttg gctttgaagg ctaaccagcg cccctgcggg gtatttttca ccaaccggct
     1561 aacttgtttg tctgcctgga taccctcctc gctcaccaga tccaggctga caggctggcc
     1621 tccagcgctg acctggatgg tttccagcac ggcatccgga ttgacacgct ggtcgaaggc
     1681 gataaagaag aggggttcca gcggctgggg gacatcatac gggtaagtgg agatcatctt
     1741 gggtgggggc gtgctgaatg tccattcgac cgcctgagcc aacctgccac cgatggccga
     1801 ttgcgtccca gcaggcacag tgacccggta cagtgtcgct tttggtaatc ggtcgatcag
     1861 ctccgagtca tattggaaag tcagcgtctt ggtacccaac catcgccatg tgcccggcag
     1921 ggctggttcc atgcgcacag gcacctgctc ggcagccagg tcggcgagcg ttgccagggg
     1981 gaccatgggc tggttgaaag tgatgttgac aaaaggcgcg attgggatct ccccttctgg
     2041 ggcaaagcgc agcacctcca ggggaccggc ttcgacctca acgggttgca cgtcgggagg
     2101 gggcggaaat tgctccgaga tggtctcgcc agggcggggc ggcgggatag gctcttgcgc
     2161 cagcctgaaa tccacctggt ctgggggctg catggctaaa gccggcagcc ggctaagaat
     2221 ttgctggatc tcgtcttcag tcaatggttc accagtggct tgcggcacgg ggacagccgt
     2281 ctgcggctgg gcctggcctt cgctcagctg gatggataca tcgggctcac ctgtttccac
     2341 ctcggatatg cctccttgcc ccgtggatat gcccgtatcc accgggattt gctgcccccc
     2401 ggaatctgcc cgcggccaac cacgtacaat cagataaaca ccggctgata ccagcaagac
     2461 gaagatgaca agcgctggga tacgatactg gctcagaaaa ctctttatat tgcgtggcat
     2521 ggccgcattc cctccttaac ccctgtgtac ttatatatat tttatacgct attcaacaga
     2581 cgtataaata tatttattcg ttccacaagc tgatgtggga ttgctcattg atgtatcagt
     2641 ttttgggtca actagcgggg taatgataat acacaatgga aaacaggctg acacctcccc
     2701 ctatttctgc acacaagcgg ggtaccgcca gatgggtcaa gaaggcaaat tgattgtcgg
     2761 tgagccctaa tgcgcaaatg gtgacagctt aaactaagga acaccccgaa ttgagatcaa
     2821 atcggggtgt tttgtgttac atatgaaatt gggaaattta acttacgggt aaaacgttgg
     2881 cagcctgcaa tcccttggga ccattctcga tggtgaactc caccttctgg ccttccatga
     2941 gattgcggta accgtccgcc atgatggcag agaagtggac gaacacatct ggaccgtcct
     3001 cgcgggagat gaaaccatat cccttgctac cattgaacca ctttactgtg ccgatgatac
     3061 gatcagccat tttcttttaa ctcctgtttt ttgaaaaaat tactcgtgat gctggctttg
     3121 ttccatcgac gcagtcaaca aaaaagcccc aggacttagc ctaaggccat agtcttcctt
     3181 acgtgcaacg gttactcact ggcatcccaa cgcaatcaat tatagcatgt gaattcaagt
     3241 ttgtcaaata aattttatac gttttgtcac cttaaaatac tggagaacgt cctccccaga
     3301 ctatctccta gcgcccgcat gctacccgtt atcccatcta caatccgctc tccagacgat
     3361 gtaagttttg taaaggcaag ccaatcccct cccatcatga catataaggc gtaaatcaat
     3421 cccagcaaaa tgattaccac caggatggat ggtaacgggt tgtttccggg ttctttcatc
     3481 gcatatcctc cacttatcac ccgcgcgcat cacttgtctt caatgccaga aggcgctagc
     3541 tttgataact tgaccgccag gtaagatggc ttttggtcac gtatctcggc ccattctttc
     3601 tgaaccacag cattgcggac aaagtgtgcg cca
//
LOCUS       DBNHOH010000325         1954 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1300717, whole genome shotgun sequence.
ACCESSION   DBNHOH010000325 DBNHOH010000000
VERSION     DBNHOH010000325.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1300717"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1143
                     /locus_tag="AC16V7_05815"
     CDS             115..1143
                     /locus_tag="AC16V7_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIR6256669.1"
                     /translation="MPHYKLAFLGFGNVGQALARLLLRKQETIQSQFGITFNVTGIAT
                     GRHGTAIDPGGISLERALGLMDGLHSLNELSASPAPKDALDFIRHCGADVLFENTPVN
                     YESGQPAVDHLRLAIEQGMHAITANKGPVVHAYRDLTELAREKGRKFYFESTVMDGAP
                     IFSLFRETLPAAHLRSFRGVLNSTTNLILTRMEGGEAFDQAVAYAQSIGIAETDPSGD
                     IDGWDAAVKVAALITVLMGVPFKPSEVERQGIREITLQDIEQARLQGKRWKLVCSAER
                     QGKDVRGRVAPEMVGMDSPLFGVDGTTSIIQFESDVLGLLSVIEADPGPHTTAYGLLA
                     DFLNAVRN"
     gene            complement(1239..1874)
                     /locus_tag="AC16V7_05820"
     CDS             complement(1239..1874)
                     /locus_tag="AC16V7_05820"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6256670.1"
                     /translation="MNIQLVLFDMDGVIFEGENFWLELHQHYGTQKKGVAFANKYLDN
                     DYDQLVRLVAGNLWKGKPASAVQTMVEQRRYQPGIFDVFEFLHAKSILSAIISSGPYE
                     LALRAQRDLGIPIVKANHLVVENGLIQGDVEVMVPDADKARVGREVMEELGIHPSNVA
                     FIGDTDSDIELAKIVNLPIAYNAQSQALIKVCKVVLNYGELGKLRDILQQF"
BASE COUNT          487 a          553 c          471 g          443 t
ORIGIN      
        1 gaagaaatcg cagcgctgat ccagcgcgca aatcgcatac ttgagaatgg ttgttaccca
       61 aatcctgacc tcagccggag gcagtatccc tggcctcccg tataggagaa tgaaatgcca
      121 cactacaaac tcgcttttct aggctttggc aacgtcgggc aggctcttgc acgcctgttg
      181 ctcagaaagc aagaaacgat ccaatcgcaa ttcgggatta ccttcaacgt gaccggtatc
      241 gccaccggcc gccacggaac agccattgac cccggtggga tcagcctgga gcgagcctta
      301 gggcttatgg atggcctcca ttcgctgaat gaactctccg cctccccggc tccaaaagat
      361 gccctagact tcatccgtca ctgcggtgca gacgtgctct tcgagaacac cccagtcaac
      421 tatgaaagcg gtcagccagc cgtcgaccac ttacgactgg cgatagagca gggtatgcac
      481 gccattacag ccaataaagg tccagtggtg cacgcctacc gtgacctgac agagctagcc
      541 agggaaaagg gacgtaaatt ctacttcgaa tccacggtga tggacggtgc gccgatcttc
      601 tctttgttta gagagacact gccagccgcc catctgcgct ctttccgtgg cgtgcttaac
      661 tccaccacca acctgatctt gacgcgcatg gaaggcggcg aggcttttga tcaggctgtc
      721 gcctatgcac aatcaattgg catcgctgag accgatccca gcggcgatat cgatggttgg
      781 gatgcagcag tcaaggtggc cgccctgatc acggtgttga tgggtgtgcc cttcaaacca
      841 tcagaagtag agcgccaggg aatccgcgag atcacattgc aagatattga acaggcacga
      901 ttgcaaggta aacgctggaa gctggtctgc tcggctgagc gccagggaaa agatgtgcgc
      961 gggcgcgtcg ccccagagat ggtaggcatg gattcccccc tcttcggcgt ggatggtacc
     1021 accagcatca tccaattcga gagtgacgtg ctcggcctgc tctcggtcat cgaggcagac
     1081 cctggaccgc ataccaccgc ctacggcctg ctagccgatt ttctcaacgc ggtcagaaat
     1141 taaaccaggt tattcttggg gtaaaagaac atcataagct tgagccatcc tgcccattag
     1201 ggagaaccgc tcctaaatct cagcaggact gattcggatt agaattgctg tagaatatct
     1261 cgaagcttgc ccagttctcc gtaattcagg accaccttgc aaactttaat caaggcttgt
     1321 gattgggcat tgtaggcaat aggcagattc acgatcttcg ctaactcaat atctgaatcc
     1381 gtatccccaa tgaatgcgac gtttgaaggg tgaatgccca actcttccat cacctcgcgg
     1441 cctacacgcg ctttatctgc atccggcacc atcacttcta catccccttg gatcaagcca
     1501 ttctctacaa ctaggtgatt agccttaact atcgggatgc ccagatctcg ctgtgctcta
     1561 agagccagct cgtacggacc agagctaatg atcgcagaga ggatggactt tgcatgcaga
     1621 aattcaaaca catcgaagat tccaggctgg taacgtcttt gttccaccat ggtttgtact
     1681 gcagaagccg gcttgccctt ccagagattt ccagcgacca aacggactag ctggtcataa
     1741 tcattgtcca aatatttatt cgcaaatgcc accccctttt tttgagtacc gtaatgctgg
     1801 tggagctcaa gccaaaagtt ttccccttcg aaaatcaccc catccatatc aaatagcacg
     1861 agttgaatat tcataacacc acaaattaaa cgtgttaatt ggggcgcgta agggtcatct
     1921 cacccgccat ctcacttacg cccacaacta tcca
//
LOCUS       DBNHOH010000326         3995 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1685948, whole genome shotgun sequence.
ACCESSION   DBNHOH010000326 DBNHOH010000000
VERSION     DBNHOH010000326.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1685948"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /gene="rpsT"
                     /locus_tag="AC16V7_05825"
     CDS             <1..261
                     /gene="rpsT"
                     /locus_tag="AC16V7_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIR6256671.1"
                     /translation="SEIKRNKQNEKRRLRNRIFRGRARSAVKRARLAIDANEVDSARQ
                     AALEAISALDKAAEKGVLHKNNAARRKSRLMRHLATLEKQSD"
     gene            484..2292
                     /gene="argS"
                     /locus_tag="AC16V7_05830"
     CDS             484..2292
                     /gene="argS"
                     /locus_tag="AC16V7_05830"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIR6256672.1"
                     /translation="MFEAEQQAIEKSIIAYCAQSGLPEPGPLQWSPIPFSGEWGISTS
                     FFQLAAQEARQGKGSGVPVPQRAAEIAAGVAAYLGTPPGYSRVEAVKAYLNCYFSASE
                     YARRVVDTVLTQGADFGRGAVKGERVMVEYANTNTHHSFHIGHARNALLGEALARLVE
                     FAGFETIRASYPGDIGLGVITVVWAYQKFYLGQEPSGVHERGQWLLKIYVEATKLLEP
                     REDETPEERAQRESYETERREIYRRWDANDPRVRELWRMTRQWSLDEQLAILDMLDIH
                     IDVWFFESEVDESAKTIVDELVALGVADDERSNAGPVIVKIDEKLGLKKEKYRSNIIL
                     RSDGTTLYLTKDLALAKEKFEKHHVDRSIYVIDVRQSLHMQQTFKILELWGFKQAEKC
                     FHLGYGFVSLPEGAISARRGRIVLFKDVADEAIRRVLSVIAEKNPEMPESDRQVVARQ
                     VGLGALAYSMLSVDTNKDIVFDWDAALNFDGHTAPYIQNAYVRANSILKKAGGLPPHS
                     SFDMPFSSHEVQLIDLISRFPQVVQSAAGEYRPLVMANYAYDLASTFHSFYHVVPVIQ
                     ADTEAMRDARLRLVAAARQTLANALRLLVIQPPEVM"
     gene            2369..3688
                     /locus_tag="AC16V7_05835"
     CDS             2369..3688
                     /locus_tag="AC16V7_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HIR6256673.1"
                     /translation="MSPTRILIMGAAGRDFHNFNVFYRDNPDYEVVAFTATQIPDIEG
                     RLYPPELSGPHYPKGIPIFSENDLVKLIRELQVDMVDFAYSDVPHEYVMHKASLALSA
                     GADFHLLGMGSTQLKSSKPVISVCAVRTGSGKSQTTRRVSLILRGMGYKVAVIRHPMP
                     YGDLTKQVVQRFADYGDLDKYNCTIEEREEYEPHLDNGMIVFAGVDYERILRQAEQEV
                     DIILWDGGNNDFPFYVSDLKIVVADPHRPGHELSYHPGETNARSADVFVINKVDTAPP
                     ENVIKVREHLRSLNPNAVMIEGASPLFVDDPGAIQGKRVLAVEDGPTLTHGEMAYGAA
                     WVAARRFGAAEIVDPRPFAVGSIKATFEKYPTIGAVLPAMGYGGQQIKELEETINRSN
                     ADLVLIGTPIDLNRLVKINKPNQRVRYELQEIGQPTLHDLLKAKFGK"
     gene            3748..>3995
                     /locus_tag="AC16V7_05840"
     CDS             3748..>3995
                     /locus_tag="AC16V7_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011431022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015225 - biotin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015878 - biotin transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HIR6256674.1"
                     /translation="MENTLALTITHRYWPRANGLLRDILLIGAGSFLVAALAQVRIPL
                     PFTPVPLTGQTFAVLLVGAALGSRRGAASLALYLSAGL"
BASE COUNT          891 a         1076 c         1112 g          916 t
ORIGIN      
        1 tctgaaatta agcgcaacaa acaaaacgaa aagcgccgct tgagaaaccg tatcttcaga
       61 ggccgcgctc gctcagctgt gaagagagcc cgcttggcga tcgatgcaaa cgaggtggat
      121 tccgcccgcc aggctgccct ggaggccatc agcgccctgg acaaggctgc cgagaagggt
      181 gttctgcata agaacaacgc tgctcggcgc aagagccgtt tgatgcgcca cttggccacg
      241 cttgagaagc aaagcgactg attgagtaat cattaattta attggggata gaaatgcatt
      301 cagtgcattt ctatccccaa tttctcttta attctgtatt cttttagagc ggcataaacc
      361 gctctattct gaatttgact tgatgtggct aataatccac tgaaaagagg ggtaggtcaa
      421 cacggcgcct gttttatgcg tgatataatc ctggctggca tgccccatat tcaggtgtaa
      481 cccatgtttg aagcagagca gcaagctatc gaaaagagca ttatcgctta ttgtgctcag
      541 agtggtctgc ctgagccagg accgctgcaa tggagcccca tccccttctc aggggagtgg
      601 ggtatttcta cctctttctt ccagttggct gcccaagaag cgcgccaagg gaagggcagt
      661 ggtgttccag taccccagcg cgcggcggag atcgccgccg gtgtggcggc gtatctaggc
      721 acaccgcctg gttattctcg cgttgaagct gtcaaggcat acctgaattg ttacttttca
      781 gcttccgaat acgctcgcag ggtggtagat actgtgctga ctcagggggc tgactttgga
      841 cgaggcgcag taaagggcga gcgcgtcatg gtggagtacg ccaacaccaa cacgcaccat
      901 tccttccaca tcggccatgc acgcaatgcc ttattgggcg aggctctagc tcgcctagtg
      961 gagtttgcgg gctttgaaac gatccgcgcc tcgtatccag gcgatatcgg cctgggcgtg
     1021 atcaccgtgg tgtgggctta ccagaagttt tacctgggac aggaaccctc tggggtgcat
     1081 gagcgcggcc agtggctgtt aaaaatctac gttgaagcta ccaaactgct cgaacccagg
     1141 gaagacgaaa ccccggaaga aagagcccag cgtgaaagtt atgaaactga gcggcgtgag
     1201 atttaccgcc gttgggatgc caatgacccc cgggtgcgag agctatggcg gatgacccgc
     1261 cagtggagcc tggatgagca gcttgctatc ctggatatgc tcgatattca catcgacgtc
     1321 tggttcttcg agagcgaagt ggatgaatct gccaaaacca ttgtggatga actggtggcg
     1381 ctcggcgtag ccgatgatga gcgctccaat gctggcccgg tgattgtcaa gatcgatgaa
     1441 aagcttggct tgaagaaaga gaaatatcgc tccaatatca ttttgcgcag cgatggcact
     1501 accctttacc tgacgaaaga cctagcctta gccaaagaga aatttgaaaa acaccatgtg
     1561 gatcgctcga tctacgtgat cgacgtacgc cagagccttc acatgcagca gaccttcaag
     1621 atcctggagc tttggggctt caagcaagcc gaaaagtgct tccatcttgg ctatggcttt
     1681 gtcagcctgc ccgagggcgc catctcggcc cggcgcgggc gcatcgtgct gttcaaagac
     1741 gtggcggatg aagccattcg ccgcgtgttg agcgtgattg cggagaagaa ccccgagatg
     1801 cccgagtccg accgccaggt ggttgcccga caggtgggcc tgggtgcttt agcctattcc
     1861 atgctttcgg tggataccaa caaagatatt gtttttgatt gggatgccgc tctgaatttt
     1921 gacgggcata ccgctccgta tatccagaac gcctatgtgc gcgccaacag catcctcaaa
     1981 aaggctggtg gtctgccacc gcattccagc ttcgatatgc ctttttccag ccatgaagta
     2041 cagctcatcg acttgatctc gcgctttccc caggtcgtcc aatcggcggc tggagagtat
     2101 cgcccgctgg tgatggctaa ttacgcctat gatctggcca gcaccttcca tagtttttac
     2161 cacgtcgttc cggtaatcca ggctgataca gaagccatgc gagatgcccg cctgcgtttg
     2221 gtggcagccg ctcgccagac acttgccaat gccctgcggt tgctggttat ccagccaccg
     2281 gaggtcatgt aggcaaaaag aacaggtgct acctttgatg ccctatcgct tggaaagaaa
     2341 atcaatcatt gaggagataa aatagcatat gtcccctacg cgcatcctga tcatgggagc
     2401 cgccggccgc gacttccaca acttcaacgt tttttatcgc gacaatcctg attacgaagt
     2461 ggtggctttc acggcaaccc agattccgga tatcgaaggt cgcctctatc cgcccgaact
     2521 gtctggcccg cactatccca agggcattcc cattttctct gaaaatgacc tggtaaagct
     2581 gatccgcgaa ttgcaagtcg acatggtgga ttttgcctac agcgatgtgc ctcacgagta
     2641 tgtcatgcac aaggcctcgc ttgctctttc cgctggggcg gacttccacc tgttggggat
     2701 gggcagcacg caactcaaat ccagcaagcc ggtaatctcg gtatgcgcgg tgcgtaccgg
     2761 ctcgggcaag agccagacca cccgccgagt atccttgatc ctgcggggca tgggctataa
     2821 ggtggctgtc atccgccatc ctatgcccta tggtgacctc accaaacagg tggtgcaacg
     2881 atttgccgat tacggtgact tggacaaata caattgcacc atcgaagagc gcgaggaata
     2941 cgagccgcac ctggataatg gcatgattgt tttcgctggc gtggattacg aacgtatcct
     3001 gcgccaggcg gaacaagagg tggatattat cttgtgggac ggtggcaata acgattttcc
     3061 cttctatgtc tccgacctaa aaatcgtggt ggctgaccct caccgccctg gtcacgaatt
     3121 aagttaccat ccaggggaga ccaatgcccg ctctgcggac gtgttcgtca ttaataaggt
     3181 ggataccgct cccccagaga acgtcatcaa agtgcgcgag catctgcgct cgctcaatcc
     3241 gaacgccgtg atgatcgagg gggcgtcccc gctgttcgtg gatgaccctg gcgcgatcca
     3301 gggcaagcgc gtgctggcgg tcgaggacgg gcccactctg acgcatggtg agatggcata
     3361 cggcgcagcc tgggtggctg cgcgccgttt tggtgccgct gagatcgtgg accccaggcc
     3421 gtttgcggta ggatccatta aggccacctt tgaaaaatat cccaccatag gggctgtcct
     3481 gccagccatg ggctatggcg gacagcagat caaggagcta gaagagacca tcaaccgctc
     3541 caatgccgat ctggtgttga tcggcacccc gattgatctc aatcgcctgg ttaaaataaa
     3601 taagccaaac cagcgtgtgc gctacgagct gcaggagatt ggtcagccca cgctgcacga
     3661 tcttttaaag gctaagtttg gtaaataggc tggtaatcca agatcctaaa gatcaccgag
     3721 atctttaggg tctttatttg gtgtattatg gaaaacacac ttgctctcac aatcacccat
     3781 cgttattggc cgcgggcgaa tggattgctg cgcgacatac tacttatcgg tgcaggcagc
     3841 ttccttgttg ctgctctggc tcaggtgcgc atcccgctgc cgttcacgcc ggtgccgctc
     3901 actggtcaga ccttcgccgt tctcctggtt ggcgctgcgc tgggcagccg gcgtggcgcg
     3961 gccagccttg cgctttatct gagcgcaggc ttgtt
//
LOCUS       DBNHOH010000327         2882 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1236895, whole genome shotgun sequence.
ACCESSION   DBNHOH010000327 DBNHOH010000000
VERSION     DBNHOH010000327.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1236895"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="AC16V7_05845"
     CDS             complement(<1..186)
                     /locus_tag="AC16V7_05845"
                     /inference="COORDINATES: protein motif:HMM:NF015418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HIR6256675.1"
                     /translation="MPEFLELLPPPEALSQLLAYLPIHPEPEEIDTEQALGRVTVEPV
                     IAPHPLPSFPRTTVDGYA"
     gene            complement(239..1159)
                     /locus_tag="AC16V7_05850"
     CDS             complement(239..1159)
                     /locus_tag="AC16V7_05850"
                     /inference="COORDINATES: protein motif:HMM:NF013226.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HIR6256676.1"
                     /translation="MADSRSQNPFVLFFRISRPLFLLGAVLIYALGAGVADYLGTSID
                     WSVYLVGQLWVIALHLSGVFLYEYYTAVSEASNPYRDPLSGGDGKIGPIRTPVTMLVT
                     AAVSLTVVATLSVWMLRELRLSPVAWLVMVLACLGAVFYSVPPVRLAFSGYGELVIAI
                     LYANLLPAFAFVLQEGDLHRLLAMSTFPLTALMLALIMAFELPDYAIDIKQDRRNLMV
                     RLGWQRGMGLHNGMIFVAFLLIGLALTFGMPLFIGLPALLTLPLGLLQIWQMRRIAEG
                     VKPNWMALKLTAVVLFATTTYLLTFAFWTR"
     gene            complement(1152..1649)
                     /locus_tag="AC16V7_05855"
     CDS             complement(1152..1649)
                     /locus_tag="AC16V7_05855"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR6256677.1"
                     /translation="MTDSPTRIAERMQVEGKRTLEFFRELRPDQWRAIMYTEGSVWTV
                     REVLAHFVSAESGMTRLVENILAGGLGSPDDFDLNAYNERKVASLKEASTDELLGQFA
                     FLREKSAALAANMNPDDLTKTGRHPWLGMASIADILKMMYRHNQIHLREIRRMMNSSE
                     PEQDG"
     gene            complement(1790..2137)
                     /locus_tag="AC16V7_05860"
     CDS             complement(1790..2137)
                     /locus_tag="AC16V7_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR6256678.1"
                     /translation="MPQRRTSPSILPFVLVALGLVLILGAGVWYYNMTLSPPELVQPS
                     SPVEEENYPDIARVSLADAKAAYDTGSAVFVDVRSADSYAQSHIPGALSIPLAELPNR
                     ISELNPSDWIITY"
     gene            complement(2286..>2882)
                     /locus_tag="AC16V7_05865"
     CDS             complement(2286..>2882)
                     /locus_tag="AC16V7_05865"
                     /inference="COORDINATES: protein motif:HMM:NF013761.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HIR6256679.1"
                     /translation="VGTYIRIDMEDSPWVDKTLALYRKAHLEHGFEHLGVVIQSYLFR
                     SEQDIHSLCEECTGVRLCKGAYKEPPDVAFPKKKDVDANYDLLARLMMDSALQRGCPQ
                     ISDDGRIPPPPVFATHDEQRVINALNYAQQIGLPKKAMEFQMLHGIRRDLQQKLAADG
                     YPVRIYVPYGTEWYPYFIRRLAERPANLWFFVSNFFRR"
BASE COUNT          746 a          786 c          737 g          613 t
ORIGIN      
        1 ggcataacca tccacagtgg tgcgcgggaa agatggcaaa gggtgcggtg caattacggg
       61 ttctaccgtc accctgccta acgcctgttc agtgtcaatc tcttccggct caggatggat
      121 gggtaaatac gctaatagct gggataatgc ctctggggga gggagaagtt ccaaaaactc
      181 gggcatactt gatggtcatc cgggtttgtc gattcgtgaa taatctacga atcacatact
      241 atcttgtcca aaacgcgaag gtcaaaagat aggttgtcgt cgcaaaaagc actaccgcgg
      301 tcagtttgag cgccatccag ttgggcttga ccccctcagc gatgcgccgc atctgccaga
      361 tctgaagcaa gcccagtggc aaagtcaaca acgctggcaa gccaatgaac agcggcatgc
      421 caaaggtaag ggccaggcca atcagcagaa acgcaacgaa aatcatgccg ttgtgcagcc
      481 ccatcccgcg ctgccaaccc aggcgcacca ttaaattgcg ccgatcttgc ttaatatcta
      541 ttgcatagtc cggcaactcg aatgccatga tcaaagccag catcaaggca gtcagcggaa
      601 aggtgctcat cgccagcaaa cggtgtaaat caccttcttg cagaacaaag gcaaatgcgg
      661 gcagcaaatt agcatacagg atggcgataa ccaattctcc gtatcccgag aatgccaggc
      721 ggaccggggg tacagaatag aacaccgcgc ccaggcacgc cagcaccatg accagccagg
      781 ctaccggtga taggcgcaac tcgcgtaaca tccacacgct caacgtggca accactgtca
      841 ggcttactgc agctgtcacc agcatggtga caggtgttct tattggacca atcttcccat
      901 ccccgcccga caatggatcg cggtaaggat tgctcgcctc actaactgct gtgtaatact
      961 catacagaaa caccccactc aaatgcaggg cgatcaccca caactgcccg actagataaa
     1021 cactccaatc gatgctcgtg cccaggtaat cagctacccc agcgcccaag gcatagatta
     1081 agacagcacc tagcaggaag agcgggcgcg agatgcgaaa aaataggaca aacggatttt
     1141 gcgaacggct atcagccatc ttgttcgggt tcgctggaat tcatcatcct gcggatttct
     1201 ctgaggtgga tctgattatg gcggtacatc attttcaaga tgtccgcaat agatgccatg
     1261 cccagccagg gatgtcgtcc tgtctttgtc aggtcatccg ggttcatgtt ggctgccagc
     1321 gcagcgcttt tttctcgaag aaaggcaaat tgccctaaca gctcgtctgt ggaggcttcc
     1381 ttcaagctgg ctaccttgcg ctcattatag gcattgagat caaaatcatc aggtgaccca
     1441 agccctcccg ccaggatatt ctccacaaga cgagtcatcc ccgactcggc agacacaaaa
     1501 tgggccaata cctcccgcac ggtccacaca gaaccttctg tgtacatgat ggcgcgccac
     1561 tggtctggcc ttaactcgcg gaaaaactca agggttcttt taccctcgac ctgcatgcgc
     1621 tccgcgatac gggtgggcga gtcagtcatt cgaatgttcc ttatggctcc atgggatagc
     1681 cagcgttgga ccaggcgacg aatccgcctt tgacaggcat gactgaattg aagccgttgt
     1741 tttgcagtag ttgcgccgca cgggcgcttg actcttcagc cggtcaggtt cagtaagtga
     1801 tgatccagtc tgacggattg agttcactga tccggttggg cagctcagcc aaagggatag
     1861 ataatgcccc ggggatgtga ctctgggcat aagaatcagc gctgcgcaca tctacaaaca
     1921 cggcgctacc tgtatcgtag gctgccttgg catctgcgag gctgacgcgg gctatatcag
     1981 ggtaattttc ttcttctacc ggagaactgg gttgaactaa ctctggaggg ctcaaagtca
     2041 tgttgtagta ccacacgccg gcacccaaga tgagcaccaa acccagagct accagaacaa
     2101 acggcaagat ggaaggagag gtacgtcttt gtggcatgaa acctccatca gaattgacgt
     2161 ttaaagatac gctatcagcg agatgatacc ataaatcccc cttgcttcag attaacccca
     2221 tggataaacc ggggcaaacg catttgggcg atttcccacc actgaggaga gaaaccaggg
     2281 tgccgctacc tgcgaaagaa attcgagaca aagaaccaga ggtttgcagg acgctcagcc
     2341 aagcgacgaa tgaaataggg gtaccactct gtgccatatg gaacgtaaat gcgcaccggg
     2401 tagccatctg cagccagttt ttgttggaga tcacgacgga taccgtgtag catctgaaac
     2461 tccatcgcct tcttcggcaa tccaatttgc tgcgcgtagt ttaaggcatt gatcacgcgt
     2521 tgctcatcat gggttgcaaa aactggcgga ggaggaatcc taccgtcatc gctgatctgc
     2581 gggcagcctc gttgcaaggc actatccatc atcaggcgtg ccaggagatc atagttggca
     2641 tccacatcct ttttctttgg aaaagccaca tcgggcggtt ccttgtaggc ccctttacac
     2701 agacgcacgc cggtacactc ctcgcacagg gaatgaatat cctgctcgct gcggaacagg
     2761 taagactgaa tgactacgcc gagatgttcg aagccatgct cgaggtgtgc cttccggtag
     2821 agagccagag tcttatccac ccagggcgaa tcctccatat caatacgaat gtaggtaccc
     2881 ac
//
LOCUS       DBNHOH010000328         6683 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_146493, whole genome shotgun sequence.
ACCESSION   DBNHOH010000328 DBNHOH010000000
VERSION     DBNHOH010000328.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6683)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6683)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6683
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_146493"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="AC16V7_05870"
     CDS             <1..390
                     /locus_tag="AC16V7_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6256680.1"
                     /translation="ESKRWIKQLEVKTPSGELPAKSLSGGNQQRLVLAKWLASKPKIL
                     ILNGPTVGVDVGSKAEIHELIRSLARQGIGILLISDDIPELLMTCHRILLMRAGRIVE
                     EFKREETTEQELSSELVGAAASPTNSH"
     gene            401..1414
                     /locus_tag="AC16V7_05875"
     CDS             401..1414
                     /locus_tag="AC16V7_05875"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6256681.1"
                     /translation="MLNKFIKSSEFIIFLILVALSLVVGLINPAFFSISTVFDILRSS
                     IVFFIMAFGLLPIVIAGGIDISFVAIAAVTSYSTHMFLLNRGYQGGILLYYIIACTMG
                     LLAGLLNGFLVTRFKLPVFDVSLAAFTMWYGFTLFFVGATANFDLPSGTVGYYGKFII
                     TAQDPLVGATGLHESFIYVVVIGLLIWWFLNYTTIGRGVFAIGGNREVAVRSGFNITR
                     IMLITFAIMGVLSAIAGVTQAFLSRYFNPVIFITQPLDVLAAVILGGAAITGGRGTVF
                     GTALGVLLIQLINRTLILTGIPVEWQRLVVGIILILFTSLPALRERRAKRIGHTTELT
                     QAE"
     gene            1437..2474
                     /locus_tag="AC16V7_05880"
     CDS             1437..2474
                     /locus_tag="AC16V7_05880"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6256682.1"
                     /translation="MDTKSNPSTLMKSLSTLIKKIQMGSQTALLLGVFVVLFAFFAVT
                     EDRFLTVRSVTSMGFQLPELGVLSLAMMLSILTGGINLSVVATSNLAAVLAGFFMVKF
                     IPPDASPQQIMLYIAIALLIALVVGWLSGVINGFLIAHVGVPPILATLATLTFYTGIS
                     TGLTGGATVTGFPEQLSGIGNETFFGIPIPFLVFVLLTIYTYFLLNRTTFGFKARMLG
                     SNPIASNFSGIDNKSIIMNTYIVSGVFSAITGILIMSRTMSAAYEYGSTTYVLLTILI
                     AVLAGILPGFGHVVDIFIAVLILQILSTGFHMVLVGIRGSSFFKDFSWGVLLIIIFVI
                     NYFTRFRKAHE"
     gene            2506..3573
                     /locus_tag="AC16V7_05885"
     CDS             2506..3573
                     /locus_tag="AC16V7_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR6256683.1"
                     /translation="MDKRWAQLGDLLVNYSMAVKPGEKVMIAFVEVETYPLVHAIYRS
                     CLQAGALPQVQFLSEELNRLVLVHGTPEQIGWVPEIEAYGMEWADVYFGLRGAHNLDV
                     FWDIPAEKLSLLRQAMGKISTLRWQKTRWCLLRVPNAALAHQAGVDEETITDMFFNAC
                     FLDWPVVSQEWRRWADELTKGDQIRIVGKGTELSFSTEGRTWDVAAGHSNMPDGEIAT
                     APVESTIDGVIYFDFPGVLGGRLVHDIRLRWEQGKLVEATSSNNQDFLRSVLNTDAGA
                     SLIGEFAIGTNPEVNYFCKDILIDEKIGGTVHIALGRAYPKVGGTNQSAIHWDIIKDM
                     RQEGEIYLDGELVFRNGKVLL"
     gene            3597..5132
                     /locus_tag="AC16V7_05890"
     CDS             3597..5132
                     /locus_tag="AC16V7_05890"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HIR6256684.1"
                     /translation="MSNRFLVGVDIGTYSSKGVLVKTDGTVVASHTVPHGMDMPKPGY
                     FEHDADQVWWHDFVVITKNLLSSSGIDPKQILGIGTSAIGSCVLPIDENGKPLRPGIL
                     YGIDTRASTEIEYLERELGKHEIFRTSGSHLSSQASGPKVLWIRNHEPEIYQKTRWFL
                     TSEAYLVHKLTGKASIDIYTAGGYAPLYDVRSREWISEVAELITPLERLPKAYWSYEI
                     VGEVTAEAAQETGLAEGTPVVAGTTDAAAEAVSAGVAEFGDMMLMFGSSIFFIMKTAE
                     LIKTQSFWSSNFLEEGSFAFLGGMSTSGSLTTWFRDQLAHYEIEKEKEGGENAYAVLA
                     SLASASPIGSNGLIALPYFEGERTPLHDPKAKGVWFGLSLKHTKGDIYRAILEGVAFG
                     IRHNVEVMGEEGVQPARILAVGGGTKNPLWLQIVADVCNIDLVVPEQQIGASYGDAFL
                     AATGVGLFKDLSEIKQWVRIKETIHPNLESRKLYEINYQIFRDLYASTKHIMHRLSDH
                     LAG"
     gene            5268..6083
                     /locus_tag="AC16V7_05895"
     CDS             5268..6083
                     /locus_tag="AC16V7_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006058203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA family protein"
                     /protein_id="HIR6256685.1"
                     /translation="MKTWIDEIFGVKKPIIAMCHLWPLPGDPYYDKKGGLQEVIEWAR
                     KDLTALQDGGVDAVMFSNEFSMPYLTKVHAVTVACMARVIGELLTHIRVPFGVNVLWD
                     PSASLDLAVATGASFVREIFSGVYASDFGLWNPNCGEIVRHQHAIGAENVKLLFNIVP
                     EAAQYLAKRDVVEIAKTTVFNHRPDALCVSGLTAGSQTDVSVLRKVKDAVPETPVFAN
                     TGVRLENIDEQLSIADGAVVGTTFKFEGDFENHVDEQRVWAFMEKVRSFRKSI"
     gene            6097..6585
                     /locus_tag="AC16V7_05900"
     CDS             6097..6585
                     /locus_tag="AC16V7_05900"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6256686.1"
                     /translation="MAEYHFFTTWDVDAPIDAVWDVLSDATRYPTWWKYVISVTELEP
                     SQPDKKGGLFLYKWGTALPYTLEFKMRVTEYEPPRLMEGHARGELDGVGRWELIEKGG
                     FTRVTYDWRVRTTKAWMNLLAPLTKSAFSWNHDVIMTEGGRALARRLGVNLRGIKNET
                     LK"
BASE COUNT         1522 a         1614 c         1822 g         1725 t
ORIGIN      
        1 gaatcgaagc gctggatcaa acagctcgag gtgaaaacac cttcaggcga attgcctgcc
       61 aagagtcttt ctggtggcaa tcagcagcga ttggtgcttg ccaagtggct ggcaagcaag
      121 ccgaagattc tgatcttaaa tggaccaacc gtaggagtgg atgttggttc gaaagcggaa
      181 attcacgagc tgatccgctc tttagccagg cagggcatcg gtatcttatt gatctccgac
      241 gatatccctg agcttctgat gacctgccat cgtattctat tgatgcgtgc tggaagaatc
      301 gttgaagaat tcaagcgcga ggagaccact gaacaggaat taagctctga actggtcggt
      361 gctgccgcct ctccaactaa ttcacactag agggtgtgcc atgttaaata agtttatcaa
      421 gagcagtgaa ttcattattt ttctcatcct ggtagccctg tccttggtgg tcggcttaat
      481 caatccagca ttcttttcca tatctacagt atttgatatc ctgcgctcgt ctattgtctt
      541 ctttatcatg gcctttggcc tgctacccat tgtcattgcg ggcggcatcg atatctcctt
      601 tgtcgctatt gcggctgtca cttcgtattc cactcatatg ttcctcctta acagaggtta
      661 ccagggtggc atcctgttgt actacatcat agcttgtacc atggggctac tagcgggttt
      721 attgaacggt tttctggtta ccaggtttaa gctcccggtc tttgatgtat ccttagccgc
      781 cttcaccatg tggtacggtt ttaccctctt ctttgttgga gcaactgcta acttcgatct
      841 accttcgggc acggtcggat attacggcaa gttcattatt actgcccagg atccactggt
      901 tggtgctacg ggcttgcacg aatcttttat ctatgtggtt gtcatcgggt tgttgatctg
      961 gtggtttttg aattacacca ccatcgggcg gggggttttt gccattggcg gcaatcgtga
     1021 ggtggctgtt cgctcgggtt ttaatatcac ccgcatcatg ctgatcacat ttgccatcat
     1081 gggggttctc tcagcgatag caggggttac ccaggcattc ttgagcagat atttcaaccc
     1141 agtgatcttt attacccaac cactggatgt gctggcagcg gttatcttag gcggcgcagc
     1201 catcacaggt gggcgcggca cggtctttgg cacggcatta ggtgtattgc tcatccagct
     1261 cattaaccgc acactgattt tgaccggcat cccagtcgaa tggcagcgct tggtcgtggg
     1321 tatcatctta atactcttta cttcgcttcc agcgctaaga gaacgccgcg ccaaacgaat
     1381 cgggcacaca accgaattaa ctcaggcaga gtgattacat taacaatggc atgattatgg
     1441 atacaaaatc aaacccttcc acattgatga aaagtctttc caccttgatc aagaaaatcc
     1501 agatgggcag tcagaccgct ctgctgctag gcgtattcgt cgtgctgttt gccttcttcg
     1561 ctgtaacaga ggaccgcttc ttaaccgtgc gcagcgtcac ctctatgggc tttcagcttc
     1621 ccgaactggg cgttctttcg cttgctatga tgctcagcat cctgaccggc ggcatcaacc
     1681 tctcggtggt tgcgacctcc aacctagccg cggtgctagc tggctttttc atggtcaagt
     1741 ttatcccccc ggatgcctcg cctcagcaga ttatgctcta tatcgccatt gcgctgctta
     1801 ttgcgctggt cgttgggtgg ctcagtggcg tgatcaatgg ctttttgatc gctcatgttg
     1861 gcgtgccgcc gatcttagcc acgcttgcca cgctcacttt ttacaccggc atatctactg
     1921 gcttgaccgg tggtgcaacc gtgaccggtt ttcctgaaca gctctcgggg attgggaacg
     1981 agacattctt tgggattccc attcctttcc tggttttcgt cctgttgacc atttacactt
     2041 attttctcct caaccggaca acttttggct tcaaagcgcg catgcttggc tcgaacccga
     2101 ttgcatctaa tttttctggc atagacaata aatccatcat catgaatacc tatattgtca
     2161 gcggcgtttt ttcggctatt actggtattt tgatcatgag ccgcaccatg tctgcggcgt
     2221 atgaatatgg ttcaacaacc tatgtgttgc tgactatcct catcgcggta ctggcgggca
     2281 ttttgccagg gtttgggcat gtggtcgata tctttatcgc cgtgctcatc ctgcaaatcc
     2341 tgtccaccgg ctttcacatg gtgctggttg ggatacgcgg cagctctttc ttcaaggatt
     2401 tctcgtgggg tgtcttgctc attatcatct ttgtcattaa ttatttcact cgcttccgga
     2461 aagcccatga gtgaaagcgc acgctaggtt aggagtgcat cgatcatgga taaacgttgg
     2521 gctcagttgg gtgatttgtt ggtgaattac tctatggcgg tcaaacccgg tgaaaaggtg
     2581 atgatcgcct ttgttgaagt ggaaacctat ccactcgtac acgccatata tcggtcttgc
     2641 ctccaggctg gagcgctccc acaagtccaa tttctctcag aagagttgaa ccggctggta
     2701 cttgtacacg gaactcccga gcagatcggc tgggtgccgg agatcgaagc gtatggcatg
     2761 gaatgggccg atgtctattt cggcttacgg ggcgcccata atctggacgt gttttgggat
     2821 atccctgccg aaaagctctc gctcctgcgc caagccatgg ggaagatctc caccttgcgt
     2881 tggcaaaaaa cccgttggtg tttgctgcgc gtgccgaatg ccgccctggc acaccaggct
     2941 ggtgttgacg aagagacaat cacagatatg tttttcaacg cctgtttcct ggattggcct
     3001 gtcgtgagcc aggagtggcg gcgttgggcc gatgaattga ccaaggggga tcaaatccgc
     3061 attgtcggca aagggacaga attgagcttc tccaccgaag ggcgaacatg ggatgtcgcc
     3121 gctggccaca gcaatatgcc ggatggggaa attgccactg cgccggtgga aagcaccatc
     3181 gacggtgtca tctatttcga ttttccgggt gtgctaggag ggcggttggt gcatgatatc
     3241 cgcttgcgct gggaacaagg gaaattggtt gaagccacat cctccaataa ccaggatttc
     3301 ctgcgctctg tgcttaatac ggacgcgggt gcaagcttga tcggtgagtt cgccatcggc
     3361 acgaacccgg aagtgaatta cttctgcaaa gatatcttga tcgatgaaaa gattggcggc
     3421 accgttcaca tcgctttggg tcgagcctat ccaaaagtag gcggcacgaa ccaatctgct
     3481 attcactggg acattatcaa agacatgcgc caggagggcg agatctatct ggatggcgag
     3541 ctcgtcttcc ggaatggcaa agtgcttctt tagatcctcg cttggagaga gggagaatgt
     3601 caaatcgctt tctggttgga gttgatatcg gcacgtattc ttccaaaggt gtgctagtga
     3661 agacggatgg cacagtcgtc gccagccata ctgtgccgca tggcatggat atgcccaagc
     3721 cgggttattt tgagcacgat gccgaccagg tctggtggca tgattttgtc gtaatcacaa
     3781 aaaatctgct ttccagcagt ggtatcgatc cgaaacagat tctgggcatc ggcacgagcg
     3841 caatcggttc gtgtgtatta cctatcgacg aaaatggcaa gccattgcgt ccgggcatcc
     3901 tgtatggcat tgatacacgc gcctcgacag aaatcgagta tctggagagg gaattgggga
     3961 agcatgagat attcagaacc agcggctcgc atctgagttc gcaagcttct gggccgaagg
     4021 ttttgtggat acgcaatcac gagcccgaaa tctaccagaa aacacgctgg ttcctgacca
     4081 gcgaagccta tctggtgcac aaactgaccg gaaaagcttc gattgatatc tacactgctg
     4141 gggggtatgc tcctttatac gatgtccgct cgcgagagtg gatatcagag gtcgctgagc
     4201 taatcactcc cctcgagcga ctgcccaagg cctattggag ttacgagatt gtcggagaag
     4261 tgaccgccga ggcggcccaa gagactggtc tcgccgaggg cacgccggtg gtggcgggca
     4321 caacagatgc agccgcggag gcggtcagtg ctggagtagc ggaattcggc gacatgatgt
     4381 tgatgttcgg cagcagcatt ttcttcatca tgaaaaccgc cgagctgatc aagacccaga
     4441 gtttctggtc gtccaacttt ttagaggaag gctcgtttgc cttcttgggt gggatgtcca
     4501 cctctggaag tctgaccacc tggttccgtg accaattggc ccactacgag atcgaaaagg
     4561 aaaaagaggg cggggaaaac gcctatgctg ttcttgcgag cttggcatct gcctcaccca
     4621 tcggctcaaa cggcctgatt gctctgcctt acttcgaggg tgagcgcacc cccttgcacg
     4681 atccgaaggc gaaaggggtc tggtttggtc tgagcctcaa gcataccaaa ggggatattt
     4741 accgggctat tttggaggga gttgcatttg gcatccggca caatgttgag gtcatgggcg
     4801 aggagggcgt tcaaccagcg cgcatccttg ccgtcggcgg aggcaccaaa aacccgttat
     4861 ggctgcagat tgtagcggat gtatgtaata tcgatttggt tgtccctgaa caacaaattg
     4921 gggcaagcta tggcgatgct ttcttagctg ccaccggtgt gggattgttc aaggatctgt
     4981 ccgagattaa gcaatgggtg cgaatcaaag aaacgatcca ccccaacctg gagtcccgca
     5041 agctgtatga gatcaattat caaatctttc gcgacctgta tgccagcacc aagcacatca
     5101 tgcacaggct cagcgatcac cttgctgggt agagaaaggc tagttagtta gacagcatgg
     5161 caaattgaat cctcatagag tgagaaagtg taattcagca aaaacccagc caagctaccc
     5221 aattgtgcta ggagaagtca gaaacaattg tgcgagaggc tactgaaatg aaaacctgga
     5281 tagacgaaat ttttggggta aagaagccga ttatcgccat gtgtcacctg tggcctttgc
     5341 cgggtgatcc ctactacgat aaaaaaggtg gcctacaaga ggtcattgaa tgggcgagga
     5401 aagatttgac cgctttgcag gacggcggtg tggacgcggt gatgttctcc aacgaattca
     5461 gcatgccgta tttgaccaag gtccatgctg tgacagtggc atgtatggcg cgggttatcg
     5521 gcgagctgct cacgcatatc cgcgtcccat ttggcgtcaa tgtgctatgg gatccaagcg
     5581 catcgttgga tttagccgta gccacaggcg ccagcttcgt gcgggagatt ttttctggtg
     5641 tgtatgccag cgatttcggc ttgtggaatc caaattgtgg tgagattgtg cgacaccagc
     5701 atgctatagg cgccgaaaat gtgaaactac ttttcaatat cgtccccgag gcggcgcagt
     5761 atctcgcaaa gcgagatgtc gtcgaaattg ccaagaccac tgtgttcaac caccggccag
     5821 atgcgttgtg cgtatccggc cttaccgcgg gctcacagac cgatgtgagt gtgctgcgga
     5881 aagtgaaaga cgccgtccca gagacgccgg ttttcgctaa cacgggtgtg cgcctggaga
     5941 acattgacga acagttgagt attgccgatg gggcagtggt ggggacaacc tttaaattcg
     6001 aaggggattt cgagaatcat gtcgatgaac agcgcgtttg ggctttcatg gagaaggtca
     6061 ggtcgtttcg caagagcata tgatggagga atcacgatgg ctgaatatca tttttttacc
     6121 acctgggatg tggatgcccc gatcgatgca gtctgggatg tgctgagcga tgcgactcgc
     6181 tatccaacct ggtggaaata tgtaataagc gtaacagaac ttgaacccag ccagccagat
     6241 aagaaagggg ggctgttcct ttataaatgg gggacggcac taccctatac tctggaattc
     6301 aaaatgcgag tgactgagta tgagcctcct cgcctgatgg aagggcatgc ccgaggagaa
     6361 ctggatgggg ttgggagatg ggagctaatt gaaaaaggag gcttcacccg tgtcacttat
     6421 gattggcgcg tgcgcaccac gaaagcctgg atgaacctgc tagcgcctct gacaaaatct
     6481 gctttttcct ggaatcacga cgtcatcatg accgaaggag gccgtgcgct ggcgcggcgt
     6541 ttgggcgtga acctgagggg gataaagaac gaaaccctga aataatccaa cgaggatgga
     6601 aagcttcaac ttttcaatca atggcttaca aggctcctac tctaccatct agctatatgt
     6661 cgtggtggag atggtgtaca att
//
LOCUS       DBNHOH010000329         2253 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1556947, whole genome shotgun sequence.
ACCESSION   DBNHOH010000329 DBNHOH010000000
VERSION     DBNHOH010000329.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1556947"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="AC16V7_05905"
     CDS             <1..222
                     /locus_tag="AC16V7_05905"
                     /inference="COORDINATES: protein motif:HMM:NF013767.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_function: GO:0043424 - protein histidine kinase binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HIR6256687.1"
                     /translation="ADGDPEGIRRIAHGLKSNGADIGAIAFSDMCKDLEMVGKSGSLD
                     GSVDLLARINAEYAKVEAALKDIQREGQI"
     gene            219..1628
                     /locus_tag="AC16V7_05910"
     CDS             219..1628
                     /locus_tag="AC16V7_05910"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6256688.1"
                     /translation="MMEEHGHILVVDDNRMNRMKLSISLEQQGYTVGLAEDGQQALDI
                     LERQSFDVVLLDIIMPGLDGYQVLERIKGDPVMRDIPVIVISALDEIDSAVRCIEMGA
                     EDYLPKPFNPVLLRARLNASLQKKKLRDLEKAYLQQEVMLRQSEKLATIGKLSAGMAH
                     ELNNPAAAAQRGAEQLKDAFLSLQKIHLKLVELDLTKEQIETLLALNGLAHEKAAKPA
                     EVDSLTRSDREYDLEVWLEEQGVENAWEIAPTLVNLGYGRGDLNKLSTDFPPSEFKTI
                     IAWLHLTYTIYSLQEEIGHGSRRVSEIVKALKAYSYMDQAPIQNVDIHEGLDNTLVIL
                     RSKLKPGIKVHRDYAADLPKIQAYGSELNQVWTNLIDNAIDAMDGVGEITLRTRHDDQ
                     WVIVDVIDNGPGIPEGIQSKLYDPFFTTKPPGKGTGLGLNISHNIIVHKHQGKITIDS
                     QPGHTRFEVRLPLEYVPVE"
     gene            1700..>2253
                     /locus_tag="AC16V7_05915"
     CDS             1700..>2253
                     /locus_tag="AC16V7_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011140483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6256689.1"
                     /translation="MAKPIILAVDDEPVVLNSVERDLRQKYGREYRVMKSESGEMALA
                     ALKQLQARGESVALFIADQRMPEMNGTQFLEQAIELFPEAKKVLLTAYADTEAAIRSI
                     NRINLDYYLMKPWDPPRENLYPVLDDLLADWKVSASPSYDGIRVAGALWSPQSHAVKD
                     FLARHQIAYQWLDVDSNPDGRALA"
BASE COUNT          585 a          535 c          634 g          499 t
ORIGIN      
        1 gcagatgggg atccagaggg gatacgaagg atagcacatg gccttaaatc gaatggggct
       61 gatattggcg ccattgcttt ctccgatatg tgcaaggact tggaaatggt tggtaagtca
      121 ggctcgctgg atggatcggt agatttgctg gctcgaatca acgccgagta tgcaaaagtc
      181 gaagcagccc ttaaggatat ccagcgggaa ggccaaatat gatggaagaa cacggtcata
      241 ttttggtcgt agacgacaac cgcatgaatc ggatgaaatt atccattagc ctggagcaac
      301 agggttatac cgttggtctg gctgaagatg gacagcaggc gttagatatt ctcgagagac
      361 aatcattcga tgtcgtgctt ctggatatca tcatgccggg gttagatggt tatcaggtgc
      421 tagaacgcat taagggcgat ccggttatgc gtgatattcc agtcattgtc atctcggcat
      481 tggatgagat cgacagcgcc gtgcgctgta tcgagatggg ggcagaagac tacctgccca
      541 agccgtttaa tccggtattg ctgcgagcgc ggctgaacgc cagcctgcag aagaaaaagt
      601 tgcgtgatct cgaaaaagca tacttgcaac aggaggtcat gctgcggcag agtgaaaaac
      661 tggcgacaat tggtaagctc agcgcgggta tggctcacga gctgaacaat ccagccgcgg
      721 ctgcccaacg cggcgcagag caattgaagg atgcattctt gagcctccag aaaatccatc
      781 taaagctggt agagttggac ctcaccaagg agcaaataga gacgctcttg gcgctcaacg
      841 ggctggcaca cgaaaaagca gctaaacccg cggaggtcga ttcactgact cgcagtgatc
      901 gcgaatatga tttggaagtc tggctcgagg agcaaggggt agagaatgcc tgggaaatcg
      961 cgcctaccct ggtgaacctt gggtatgggc gcggtgactt aaataagctc tcaacggatt
     1021 tccccccgtc tgagttcaag actatcattg catggctgca tctaacttat acgatttaca
     1081 gcctccagga agaaattgga cacggttcaa gacgcgtctc cgaaattgtg aaagctctga
     1141 aggcctattc ctatatggat caggcaccca tccagaatgt ggacattcac gagggattgg
     1201 ataacactct tgtcatactg cgcagcaagc ttaaacccgg catcaaagtt caccgcgatt
     1261 atgcagccga cctgccgaag atacaggcct atggcagtga actcaaccag gtctggacca
     1321 atctgattga caatgccatc gacgcgatgg acggggtggg tgagatcacc ctgcgcacgc
     1381 ggcatgacga ccagtgggtg attgtcgatg tgatcgataa tggtccgggc attccagagg
     1441 ggatccaatc gaagttgtat gacccattct tcaccaccaa accgcccggt aaagggactg
     1501 gtttggggtt aaatatcagt cataatatca ttgtgcataa acatcaagga aagattacga
     1561 ttgattcgca gccgggccat acccgttttg aggtgcggct ccccctcgag tatgtgccgg
     1621 ttgagtaaaa tggcgctctc ggggttatat gccgggagag caacaagaaa ttttgacttt
     1681 tagtaaggaa aagataccta tggcaaaacc catcattctt gcagttgatg atgaaccagt
     1741 tgtactgaat tcagtggaac gcgacttgag gcaaaaatat ggtcgcgagt atcgtgtgat
     1801 gaaatccgaa tcgggcgaga tggcgcttgc cgcgcttaaa cagttgcagg cgcgcggcga
     1861 atcagtcgcc ctctttatag ccgatcagcg catgcccgag atgaatggaa cccaattcct
     1921 ggagcaggca atagagctgt ttccagaggc gaagaaggtg ttgctgaccg cctacgcaga
     1981 tacagaggcg gctattcgct ccatcaaccg cattaacctg gattactacc tgatgaaacc
     2041 ttgggatcca cctcgggaga atctctaccc ggttctggat gatctgcttg cggattggaa
     2101 ggtgagcgca tccccctcct acgatggcat ccgcgtagcg ggagctttgt ggtcgcccca
     2161 gtcacacgcc gtcaaggact tcctcgcccg gcaccaaatc gcttaccagt ggctggacgt
     2221 ggattcgaat cctgatggac gcgccctggc aga
//
LOCUS       DBNHOH010000330         4479 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1492830, whole genome shotgun sequence.
ACCESSION   DBNHOH010000330 DBNHOH010000000
VERSION     DBNHOH010000330.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1492830"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..177)
                     /locus_tag="AC16V7_05920"
     CDS             complement(<1..177)
                     /locus_tag="AC16V7_05920"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR6256690.1"
                     /translation="MINRDRRIFPYLETLFAVIVWGASFIATKVALRDISPVTLVWLR
                     FGIGVVILGIVTAAR"
     gene            complement(322..771)
                     /locus_tag="AC16V7_05925"
     CDS             complement(322..771)
                     /locus_tag="AC16V7_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256691.1"
                     /translation="MYSILLAVHNVLRWVVVVLAILALVRAYRGWLGRRDWGSADRRA
                     GVLFTSFMDLQLLLGLLLYIFFSPITKAALADFGAAMSNAGARFFALEHFVYMFVAVV
                     FAHMGNAFSKKAAEPHAKHQRAAIWYTLTVLAILLGMPWMRPLFPGW"
     gene            1011..2360
                     /locus_tag="AC16V7_05930"
     CDS             1011..2360
                     /locus_tag="AC16V7_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR6256692.1"
                     /translation="MFDLSNEEILPLAQQHTFWTWSAQGRVEPIPVTRAKGVHFWDAE
                     GKRYLDFNSMTMCVNIGHGEERVVEAIAEQARQLPYAAPNMATKPRALLGKLLSEITP
                     GDLDHFLFTLGGADANENAIKLARATTGKHKILARYRSYHGATAGAMAATGDPRRVTW
                     EPNLMTGVVHFLDPYRYRSTFHRSNPDISESEFTQDYLNHLEEIITYEGADSIAAILL
                     ETVTGTNGVIIPPDGYLQGVRALCDRYGILLIADEVMSGFGRTGKWFAVDHLGVVPDL
                     MTMAKGLTSAYAPLGAVGMRPQIAAFFNERVFQSGLTFTAHPISLAAAIANIRVMQDD
                     HIVEHAAAMGPVLRRMLTDLGEQHPSVGDARSIGLFGILELVRDRRTKEPMAPYDGSS
                     AEMVALRKYCLEHGLFLYTHWHTVLIIPPLIITEEQLAEGFEILDKALEITDAAANP"
     gene            2448..3875
                     /locus_tag="AC16V7_05935"
     CDS             2448..3875
                     /locus_tag="AC16V7_05935"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HIR6256693.1"
                     /translation="MSAQPAEKPSSPPLPSAYLERMQRLLGGEFEAFLQSYQQPPSTG
                     LRVNTLKISPQAFQANFPYPLSPIPWCPSGFFLKETPTSPGKHPYHAAGLYYLQDPSA
                     MIVAELLRPQAGELVLDIAAAPGGKSTHLASWMGGDGVLVANETHPQRVWELAENLER
                     WGARNTVILNETPARLAQALGDIFDKVLLDAPCSGEGMFRKNEMSRLEWSPQRVASCA
                     LRQSAILRETAGLVRPGGTLVYATCTFAPEENEAVIAMFLKENPHFELVPALPVPNVA
                     PGQPKWANFDARKKFEDTYTAALKGAVRLWPHHGAGEGHFIAMLRRTDQTNRDKKKPW
                     QPPAPSAEAARLFGEFCSDHLQWNFETGSLVQIGSYLYQIPQGLPKLGHLKTIHPGLW
                     LGTVKTGRFEPSHALALALPVEQAQKVIALDFDEANTYLRGETQKISGADGWALMTLD
                     GFPLGWGKRVKGIVKNFYPKGLRWR"
     gene            complement(3876..4259)
                     /locus_tag="AC16V7_05940"
     CDS             complement(3876..4259)
                     /locus_tag="AC16V7_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256694.1"
                     /translation="MTKYTQYQSRGRAPARPWRVHPVWRGIGCVMMILIPVIAYAGAV
                     RLVEENLKQGWLPVPAEFARAITLPVLGSVQYLLAYLLVTVVLSLIGFGVFTALYSLI
                     YSLIGPSKYGPLDSPPIRREQRGRR"
     gene            complement(4256..>4479)
                     /locus_tag="AC16V7_05945"
     CDS             complement(4256..>4479)
                     /locus_tag="AC16V7_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR6256695.1"
                     /translation="LHPVQQAFIEAGAVQCGYCTPGFLMSAAKLLEERPHPTRDEIQQ
                     SITGNLCRCTGYYTIVNAIDRAAQLMEER"
BASE COUNT         1081 a         1312 c         1208 g          878 t
ORIGIN      
        1 gcgtgcagcc gtaacaattc ccaagataac caccccaatt ccaaatcgca gccaaaccag
       61 ggtcaccggc gagatatctc gcaaggcaac cttggtggca atgaaagaag ccccccaaac
      121 aatgaccgca aacagcgttt caagataagg gaatatccta cggtctcgat tgatcatata
      181 cagttgtgcg atagattccc catatctcac aagaggaaat atatagaggc taaaatgagg
      241 agcatagctt aatttcggcg gttacccagc ttaccactgg gaagcaagag tggagatatg
      301 agggttgggt ggggaatggc atcaccaacc ggggaacagc ggcctcatcc agggcatgcc
      361 tagcaggata gccagaacag tcagcgtgta ccagatggcg gcgcgctggt gcttggcatg
      421 tggctcagcc gctttcttgg agaaggcatt tcccatgtga gcaaaaacta ctgccacaaa
      481 catatacacg aaatgctcca gggcaaagaa acgtgcccca gcattactca tggccgcccc
      541 aaaatcagct agggcagcct tcgtgatcgg gctgaagaaa atatagagca acaacccaag
      601 caaaagctga agatccataa atgaagtgaa caacacgcca gcgcggcggt cagcgctgcc
      661 ccaatcccgc ctgcccagcc agccccggta ggctcgaacc aaagccagga tagccagcac
      721 gacgacaacc cagcgcaata cattatgaac agcgagtagt atagaataca tcgaaaggga
      781 cctccattgc agattattcc aaaaacgaga caagcacccg aatagtttag ccgaaattgc
      841 ctagcgtgag aaggtttcga ggttgattga ctattgcctt ctgtgatacc attgacacca
      901 agactagaac tcatcataaa atggttattt ggggtgtttt gcagttactg cgtcgcagca
      961 aggcaccccc tcatcttgtg tttgagaacc tttctaatca cgaggcaacc atgttcgacc
     1021 tttccaatga agagatcctc cccctggctc agcagcacac attctggacc tggtcggcac
     1081 aagggcgagt tgagccaatc ccggtgacac gcgccaaagg cgtgcacttt tgggatgcgg
     1141 aaggcaagcg ctacctggat ttcaactcca tgaccatgtg cgtcaatatt gggcatggcg
     1201 aagagcgcgt cgttgaagcc atcgccgagc aggcgcgcca gctcccctac gctgcgccga
     1261 acatggcgac caagccacga gcattattag gcaaactgct gagcgagatc acgcctggcg
     1321 acttggatca cttcctgttc accctgggcg gcgccgatgc caatgagaac gccattaagc
     1381 tggcacgggc aaccacaggc aagcataaaa tcctagcccg ctaccgctcc tatcacgggg
     1441 ccacagcggg agctatggca gccaccggcg acccgcggcg cgtaacctgg gaacccaacc
     1501 tgatgaccgg cgtggtacac ttcctggatc cctaccgtta ccgctccacc tttcaccgct
     1561 caaacccgga tatctccgag agcgagttca cccaggatta cctcaatcac ctggaagaaa
     1621 ttatcactta tgagggggcg gacagcatcg ctgctatcct gctggaaact gtgaccggaa
     1681 ctaacggagt catcattcct ccagatggtt atcttcaagg agtgcgagca ttatgcgatc
     1741 gctatggcat cctgctcatc gcggatgagg tgatgagtgg atttgggcgc accggcaagt
     1801 ggtttgctgt ggatcacttg ggcgttgtcc ccgacctaat gactatggcc aagggattga
     1861 cctcggctta tgccccgctg ggtgcggtgg ggatgcgccc acaaatcgcc gcctttttca
     1921 acgagcgcgt ctttcaaagc gggctgacct tcacagctca ccctatctcg ctggcggctg
     1981 ctatcgccaa tatccgcgtc atgcaggacg accatatcgt tgagcacgcc gccgccatgg
     2041 ggccggtgct gcgccgcatg ctcactgatc tcggcgaaca gcacccttcc gtgggcgatg
     2101 cccgctctat cggcctcttc ggcatcctcg agctggtgcg cgaccggcgc accaaggagc
     2161 ccatggctcc ctacgacggc tccagcgcag agatggttgc cctgcgcaag tattgcctcg
     2221 agcatggctt attcttatat acccattggc acactgtgct gatcatccct ccgctgatta
     2281 tcacggaaga gcagctggct gaagggtttg aaattttgga taaggcgcta gagattacag
     2341 acgcagccgc gaatccctga tgccggaaat ggatcgctac ccacctcact tattaacagg
     2401 atcacgggaa gggcagagat aaactgtcgt ctcgcgggct gtcaaagatg agcgcacaac
     2461 ctgctgaaaa gccctcctcc ccaccgctgc cgtcggccta tcttgagcgc atgcaacgcc
     2521 tcctgggcgg tgaattcgag gccttcttgc agagctacca acagccgccc agcactggcc
     2581 tgcgcgtcaa caccctgaaa atctcacccc aagccttcca ggctaatttt ccctatcccc
     2641 tctcccccat cccctggtgc ccctctggat tctttcttaa agaaacgccc acatcacccg
     2701 gcaagcaccc ctatcacgct gccgggctgt attacctgca agacccatcg gcaatgatcg
     2761 tcgccgagct gcttcgacct caagcgggcg agctagtatt agatattgcc gctgcaccag
     2821 gcggcaagtc tacccacctg gcatcctgga tgggcggaga cggcgtcctg gtggcgaatg
     2881 agacacaccc acaacgtgtt tgggagctgg cagagaactt agaacgctgg ggagcgcgaa
     2941 acaccgtcat cttgaacgag acacctgcac ggctggcaca agccttagga gatattttcg
     3001 ataaagtgct gctggacgct ccctgctctg gagagggcat gttccgcaag aatgagatgt
     3061 cccgcctgga atggtcgcca cagcgggtgg caagctgcgc cttgcggcag agtgctattc
     3121 tgagagagac ggctgggctg gtgcgacccg gcggcacact ggtctacgcc acttgcacgt
     3181 ttgcacctga agagaacgag gcagtcatcg ctatgtttct aaaggagaac ccccacttcg
     3241 agctcgtgcc ggcgctgccg gtgcccaacg tggcgcctgg acagcccaag tgggcaaatt
     3301 tcgacgcaag gaagaaattt gaagacacct acacggctgc gctaaaggga gcagtgcgct
     3361 tatggcccca tcacggcgca ggagaaggac attttattgc catgcttcgt aggactgatc
     3421 aaaccaaccg agataaaaag aagccctggc aaccgccagc cccatctgca gaagccgctc
     3481 gcctgtttgg cgaattctgc tccgatcact tgcagtggaa tttcgagacc ggctccctgg
     3541 tccagattgg ctcttacctg taccagatcc cccaagggtt gccaaaacta ggacatttga
     3601 agactatcca ccctggatta tggttgggca cggtcaaaac cgggcgcttc gagccctcgc
     3661 acgccctggc gctggccctg cctgtcgaac aggcgcagaa ggtgatcgcc ctcgacttcg
     3721 acgaggcgaa cacttatttg cgcggggaaa cacagaaaat tagcggggcg gatggatggg
     3781 cgctgatgac cttggatggc ttcccgctcg gttggggcaa gcgggtcaaa ggtatcgtaa
     3841 agaacttcta cccaaaaggc ctacgctggc gttaatcacc tgcgccctct ttgctctcgc
     3901 cttatcgggg gtgagtctaa aggtccatac ttcgacggac cgatgaggga atagataagg
     3961 gaataaagcg cagtaaaaac gccaaacccg atcaggctca agacgacagt caccagcagg
     4021 taagccagca aatattggac gctacccagc acagggaggg taatcgcacg cgcaaactcg
     4081 gcaggcacag gcaaccaacc ctgcttcagg ttctcctcaa caagccgaac tgcgcccgcg
     4141 taagcgatca cagggattaa gatcatcatc acgcagccga tgccgcgcca gaccgggtgc
     4201 accctccagg gacgtgcagg tgcgcggccg cgggattggt actgagtgta tttggtcatc
     4261 gttcctccat tagctgagca gccctgtcga tcgcattcac gatcgtataa tatcctgtgc
     4321 agcggcacag gttgccggtt atggattgct ggatctcatc gcgagtgggg tgaggcctct
     4381 cctccagcag ctttgcagcc gacatcagga accctggggt gcagtagccg cactgcacag
     4441 cacccgcctc gataaaagcc tgctgaacag ggtgcagat
//
LOCUS       DBNHOH010000331         1379 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1300961, whole genome shotgun sequence.
ACCESSION   DBNHOH010000331 DBNHOH010000000
VERSION     DBNHOH010000331.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1300961"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1368)
                     /locus_tag="AC16V7_05950"
     CDS             complement(<1..1368)
                     /locus_tag="AC16V7_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6256696.1"
                     /translation="MRSREVAHQLGIPDWEHTMLANIVDICFLLGDLVYIQAVVTELN
                     ELIQCIPNPEYSLLLNQFIEARMHRFKGEIDEALTTLRRCSESAKQSGRTRIEAKNNV
                     IWGDLLIELGKIEEAEKIIAETLQIGEYVDEEDKVIAQLLMGTVRTKQGRYDEAHQLI
                     DSIKAQIVNNPTIGTKGYLAWSEAHLAGAEENWQRAFEYFQNLDQMTSQLKLPWYRAR
                     ALVDWAAVHINRGEADDLLRSKELLEEAIQIYTDLGLPRYAARVEQIGHNLPELEFHI
                     SRLARDRYDFEDTLFSLTGNVVFANFHAARLFAQKMNQKRDLINYPERAVRPGQINAM
                     GLIDEILHLVISIYIQQRNPRAMREALAWLYDNLGQDTVDTTLLRFTEEFPPVSVYRR
                     QLESDEYLSGESGGVSHILLALEEMLMLSITNANPACGPYLELFDDSNLEKVTRYADI
                     MAGLRE"
BASE COUNT          332 a          335 c          328 g          384 t
ORIGIN      
        1 ctctctcaag ccagccatga tatcagcgta acgagtaact ttctcgaggt tggaatcgtc
       61 aaataactcc agataagggc cacaggctgg attagcgttg gtaatcgaga gcatgagcat
      121 ctcttcaagc gccagcaaga tgtgcgacac acccccgctt tcgccgctca aatactcatc
      181 cgactccaat tggcggcggt aaactgacac gggagggaat tcctcggtaa agcgtagcag
      241 ggtggtatcc accgtatcct gacccaggtt gtcgtatagc cacgccagag cttccctcat
      301 tgcgcgcggg ttgcgctgct gtatatatat ggagatgact aaatggagga tctcatcaat
      361 caatcccata gcgttaatct gcccgggtcg cactgctcgt tctgggtaat tgatcaggtc
      421 gcgcttttgg ttcatctttt gtgcaaaaag gcgagctgca tggaaattgg cgaagaccac
      481 attgccggtt agtgagaaaa gtgtgtcttc gaagtcatag cggtcgcgtg ccaggcgcga
      541 gatgtgaaac tccaattccg ggaggttgtg acctatctgt tcaacacggg cggcatagcg
      601 aggcagacca agatcagtgt aaatttggat cgcttcctcc aataactctt tggacctgag
      661 aagatcgtct gcttcacccc ggtttatgtg aaccgcggcc caatctacca gggcgcgggc
      721 tcgataccaa ggaagtttta gttgagatgt catttggtcg aggttttgaa aatattcaaa
      781 agctctctgc cagttttctt ccgcgccagc gagatgggcc tccgaccaag ctaaatatcc
      841 cttagtacca atggtgggat tattcactat ttgagctttt atcgagtcga tcaattgatg
      901 ggcctcgtca tacctgccct gcttcgtgcg tacggtgccc attagcaatt gtgcgattac
      961 cttgtcctct tcgtctacat attctccaat ctgtagcgtt tcagcgataa ttttttccgc
     1021 ttcttcaatt tttcctaatt cgatcaaaag atctccccag atgacattat ttttggcttc
     1081 tatacgtgta cgaccggatt gctttgcact ttcgctgcat ctcctcaagg ttgtaagtgc
     1141 ttcgtcaatt tctcctttga agcggtgcat acgagcttcg ataaattgat ttaacaataa
     1201 cgagtactct ggatttggaa tacattggat gagttcgttt agctccgtaa cgactgcctg
     1261 gatatagacg agatctccta agagaaaaca aatatctaca atattggcga gcatggtgtg
     1321 ctcccaatcg ggaataccta attgatgagc tacttcgcga ctgcgcatca gatgctcac
//
LOCUS       DBNHOH010000332         2601 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1172789, whole genome shotgun sequence.
ACCESSION   DBNHOH010000332 DBNHOH010000000
VERSION     DBNHOH010000332.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1172789"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="AC16V7_05955"
     CDS             <1..330
                     /locus_tag="AC16V7_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR6256697.1"
                     /translation="HRRQVQIDLGVVKWNGNNTIKGYIEKPTYEYDVSMGVYVFEPRV
                     LEFIPHNQYLDFPDLVLKMIAANEKVAGYIFEGYWMDLGRPDDYAQAAENFSVMRSQF
                     LPDECDL"
     gene            327..1475
                     /locus_tag="AC16V7_05960"
     CDS             327..1475
                     /locus_tag="AC16V7_05960"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR6256698.1"
                     /translation="MRWRVPLSDIDFGLEEEAAVLEVVRSRWLTMGAVTQEFEQEVAA
                     FTGAKHAIALTNATAALHLACLAAGLGPGDEVILPALTFVASANAVRYTGATPVFADV
                     EGEHALNVSPAACEARLTSRTRALLVVHYGGYACDMPAIMGLAKRHNLAVIEDAAHAI
                     GSRLEERQLGTWGQSGCYSFFSNKNMTTGEGGMLVTDDDEVAEKVRNLRSHGMTSLTW
                     DRHKGHAWSYDVADLGYNYRIDEIRSALGRAQLRKLARNNQVRRERTALYWELLQELC
                     PEVKLPFREHAGLSACHLLPILLPQGKWRQAFMEGMKNRGVQTSIHYPPIYHFSAYRA
                     LAAGSQAGLQRTEDLAARVVTLPLYPALSEAEVLLVSEAVRDTLAGLQ"
     gene            1602..>2601
                     /locus_tag="AC16V7_05965"
     CDS             1602..>2601
                     /locus_tag="AC16V7_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6256699.1"
                     /translation="MTQPVISVEHLSKSYQLGQINTGTFTNDLKLWWARARGKPNPFL
                     RIGEVDNGNRNGEMLWALRDVSFQVQQGEVLGIIGRNGAGKSTLLKILSRVTAPTEGR
                     IRAKGRIASLLEVGTGFHPELTGRENIYLNGAILGMSRAEVARKFDEIVDFAEVEKFI
                     DTPVKRYSSGMYVRLAFAVAAHLDAEIMVVDEVLAVGDVEFQKKCLGKMGDVAQGGRT
                     VLFVSHNMAAVQNLCGRGIVLQEGQIKYQGDIDTGVSSYLVGQKEENCSWKRKQGSNG
                     QMEINEISISLRGEQPRHTLHISLTLLSNHQHRPAFIAIDISSSFGNTIMQAIPTIV"
BASE COUNT          570 a          713 c          786 g          532 t
ORIGIN      
        1 caccgccgtc aggtgcagat cgacctgggc gtggtgaagt ggaacgggaa taacaccatc
       61 aaaggctata tcgaaaagcc gacctatgaa tacgacgtca gcatgggggt ttacgtcttt
      121 gaaccccggg tcttggagtt catacctcat aaccagtatc ttgactttcc cgaccttgtc
      181 ttgaagatga tcgcagccaa cgaaaaggtc gccgggtaca tcttcgaagg ctactggatg
      241 gatctaggcc gcccggatga ctacgcccag gccgccgaaa acttcagcgt catgcgttcc
      301 caattcctgc ccgatgagtg tgacctgtga ggtggcgcgt cccattatct gatatcgatt
      361 tcggcttgga ggaagaggcg gcggtcctgg aggtggtacg gagccgctgg ctgacgatgg
      421 gggccgtcac ccaggaattc gagcaagaag tggccgcctt cacgggcgcg aagcacgcca
      481 tcgcgctgac caacgccacc gccgccctgc acctggcctg cctggcagcc gggctgggtc
      541 cgggcgacga ggtgatcctg ccggccctga ccttcgtggc cagcgccaac gctgtgcgct
      601 acacaggtgc cacgccggtt tttgccgatg tggagggcga gcacgctttg aacgtttccc
      661 cggctgcgtg cgaggcccgt cttacgtctc gcacgcgcgc gctgctggtg gtccattatg
      721 gcggttacgc ctgcgacatg ccggccatca tgggactggc caagcggcac aacctggcgg
      781 tgatcgaaga tgctgcccac gccatcggtt cccggttaga agagcgccaa ctcggtacct
      841 ggggccagag cggttgttac agtttctttt ccaacaagaa tatgaccact ggcgaagggg
      901 gcatgctggt aacagacgat gatgaggtgg ctgaaaaagt gcgcaacctg cgctcgcacg
      961 gtatgacctc actgacttgg gaccggcata agggacacgc ctggagttac gatgtcgccg
     1021 acctgggcta caactaccgc atcgacgaga tccgttcggc gctggggcgg gcgcagctgc
     1081 gaaagctggc gcggaacaac caggtgcgcc gcgagaggac ggcgctttac tgggaattgc
     1141 tccaggagtt atgcccggag gtcaaacttc ctttccgcga acacgctggc ctttcagcct
     1201 gtcatctgct gccaatcttg ctcccgcagg gaaagtggcg tcaggctttt atggaaggca
     1261 tgaagaaccg cggggtgcaa accagcatcc actacccgcc gatctatcat ttcagcgcat
     1321 accgggctct cgccgccggt tcccaagctg gtctgcagcg caccgaagac ctggccgcgc
     1381 gcgtggtcac gctgccgctc tacccggccc tttccgaggc cgaggttttg ctggtgagcg
     1441 aggcagtgcg ggatacgctg gctggtttgc agtgagaatt ccagatttct gtcgatttgc
     1501 ggtggtcgag agtaccgcca atgtatatcc tctccaaatc ctggattcac gtttcattat
     1561 atatatttag agataggcca cttaaaatcc aagagtaata tatgacccaa cccgttatct
     1621 ccgtcgaaca cctctccaaa tcttaccagc tgggacagat caacaccggc acgttcacca
     1681 acgacctgaa gctgtggtgg gcgcgggcgc gtggcaaacc caacccattt ttacgcattg
     1741 gggaggtgga caacggcaac cgcaacggcg agatgctgtg ggcgctgcgc gacgtttcct
     1801 ttcaggtaca gcaaggcgag gtgctgggca tcattgggcg gaatggggcg ggcaagagca
     1861 cgctgctcaa gatcctctcg cgcgtcaccg cccccaccga gggccgcatt cgcgccaaag
     1921 gaaggatagc cagcctgctg gaggtgggca ccggtttcca ccccgagcta accgggcggg
     1981 agaatattta cctcaacgga gccatcctgg gcatgagccg cgccgaggtg gcccgcaagt
     2041 tcgatgagat cgtggacttt gcggaggtgg agaaatttat cgacacgccg gtcaagcgct
     2101 actcgagtgg catgtacgtg cgcctggcct ttgccgtggc cgcccacctg gacgcagaga
     2161 tcatggtggt ggacgaggtg ctggcggtgg gagacgtcga gttccagaag aagtgcctgg
     2221 gcaagatggg ggatgtggcg caaggggggc gaacggtgtt gttcgtcagc cataatatgg
     2281 ctgctgttca gaacctgtgt gggcgcggga ttgtattgca ggaagggcaa ataaaatacc
     2341 agggggatat agataccggt gtttcttctt atttagttgg gcaaaaagaa gaaaattgct
     2401 cctggaagcg taaacaaggc agcaacggcc aaatggaaat caatgaaata tcaatttcat
     2461 taagaggcga acagcccaga cacacattac atatatcgtt aactcttctc agtaatcatc
     2521 agcatagacc cgcattcata gccatcgata taagtagttc ttttggtaac acgattatgc
     2581 aagctattcc aacgatagtt c
//
LOCUS       DBNHOH010000333         2299 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1749885, whole genome shotgun sequence.
ACCESSION   DBNHOH010000333 DBNHOH010000000
VERSION     DBNHOH010000333.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1749885"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="AC16V7_05970"
     CDS             <1..921
                     /locus_tag="AC16V7_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256700.1"
                     /translation="KRLVKVASLWLVFALILSILYPARQVHDLIWALIPLWILAALTL
                     AGLFADSGENSMISLGHATILFIFMTLVWVNIAGLGQSEPGSPGYLTRLAVVGGVIAL
                     GAVTSGLIGLGWSWKTAQDGAVWGMCAILGIYTLAGMWWASQVRDPGRVELWQPFPSS
                     AGTRLIIETVTDLSAWNIGDTQALDVTLAVDSPALHWSLRTFPSLSVIPEGEQLISMG
                     SPAIVITRQDVEEPGLAAEYRGQDFVFRTYPGWFGALPPNILRWVAFRLAPLQEEQVI
                     VWARADLFPGGSSLSEESPPAFDIPGLDEP"
     gene            911..1618
                     /gene="cmk"
                     /locus_tag="AC16V7_05975"
     CDS             911..1618
                     /gene="cmk"
                     /locus_tag="AC16V7_05975"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIR6256701.1"
                     /translation="MNPRRMPNPTVIAIDGPAASGKSTLGQNLAGELGFLYFDTGVMY
                     RAVTWAALQRNISVHDEIAITTLAESLTIDVRPPSMADGRAYDVWVEGEDVTWHIRRP
                     EVEADVSEVSAYAGVRRALTLQQRKIGLRGRVVMVGRDIGTVVMPEAALKIYLDASAE
                     ERARRRYLELQARGEQPDQEAILQAMLARDRIDSTRDLAPLRPAEDAVVLVTDGLDAD
                     QVLEKAKELASQAGISA"
     gene            1728..>2299
                     /locus_tag="AC16V7_05980"
     CDS             1728..>2299
                     /locus_tag="AC16V7_05980"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR6256702.1"
                     /translation="MENIPRQGGYLIAANHVSLYDPPLVLAFWPTEPEAVGAVDIWQR
                     PGQSLLVRMYGAIPAHRGEYDRHLMNTAQAALLSGKSIMIMPEGTRSHAPGMRRAAPG
                     VAYLIEKTDVPVVPVGVVGTTDDFFQRAMRGEKPSLYMYIGAPFRLPLVDGKGEDRRR
                     RRQENADLIMAHIAALLPPEYQGVYANTTR"
BASE COUNT          468 a          553 c          686 g          592 t
ORIGIN      
        1 aagcggctgg ttaaggtcgc cagtttgtgg ctggttttcg cattaatttt atcgatactt
       61 taccctgccc gtcaggtaca cgacctgatt tgggctctca tccctctatg gatactagcc
      121 gccttgacac tggctggatt atttgcagat tcgggcgaaa attctatgat atcgctcggt
      181 cacgccacga ttttgtttat ttttatgaca ttggtctggg taaacattgc gggattgggc
      241 caatctgaac caggctcgcc gggttatctg acccggctag cagtggttgg tggtgtcatc
      301 gcgctgggtg cggtgacatc aggcttgatt ggcctggggt ggtcttggaa gacggctcag
      361 gatggcgcgg tttggggtat gtgtgccatt ttaggcatct atacactggc tggtatgtgg
      421 tgggcctccc aggtgcgtga tcctgggcgg gtggagctct ggcagccgtt cccaagcagc
      481 gctggtacga ggctcatcat agaaactgtg actgatttat ctgcctggaa catcggtgat
      541 acgcaagctt tggacgtgac tctggcagta gattcgcctg cccttcactg gtcgctgcga
      601 acatttccct ctttgagcgt gatcccggaa ggtgaacaac tgatttccat gggctccccc
      661 gcaattgtta tcacccggca ggatgtggaa gaaccaggcc tggctgccga atatcgaggt
      721 caggattttg ttttcaggac atatcccggc tggtttgggg cattacctcc taatattttg
      781 cgctgggttg cttttcgcct agcgccactg caagaggagc aagtgatcgt gtgggcgagg
      841 gccgacttat ttccgggtgg ttcatcgctc tcagaagaat ctccacctgc ctttgacatt
      901 cccggtctcg atgaacccta gaagaatgcc caatcccacg gttatcgcta ttgatggtcc
      961 ggcagcttca ggaaaatcca ctctggggca aaatttagct ggtgaactgg gttttttgta
     1021 ttttgatacg ggcgtaatgt accgcgctgt gacctgggct gctttgcagc gaaatatctc
     1081 agtacatgat gaaatagcaa taaccacctt agcagaaagc ctaaccatcg atgtgcgacc
     1141 gccatccatg gccgatgggc gagcttatga tgtgtgggta gagggtgagg atgttacctg
     1201 gcatattcgc cgacctgagg tggaagccga tgtgtccgag gtttctgctt atgcgggggt
     1261 gcgtcgcgct ttgactctcc aacagcgcaa gattggattg cgcggccggg tagtgatggt
     1321 cggtcgggat atcggcacgg tagtaatgcc agaagctgcc ctgaaaattt acctggacgc
     1381 ttcggcggag gagcgtgccc gccggcgcta tttggaattg caggcacgtg gcgaacaacc
     1441 tgaccaagag gctattctgc aggccatgct ggcgcgtgac cgcatcgatt caactcgcga
     1501 cctggcacct ttgcggcctg ccgaagatgc ggtggttctg gtcactgatg ggctggatgc
     1561 cgaccaggtg ctggaaaagg caaaagagct ggccagccag gctgggataa gtgcttgaga
     1621 tgagcggcgt ttacaaacta cctatgggta tacgcctagc acgtaggata ttaagacctc
     1681 tgtttcgagg tatttttcga ttaatcagca aggtgaatat tatcggtatg gagaacattc
     1741 ctcggcaggg ggggtatctc attgcggcga atcatgtttc attgtacgat cctcccttgg
     1801 tgctggcctt ttggcctacg gagccagagg cggtaggtgc tgtggatatc tggcagcgtc
     1861 ccggacagtc gcttctggtt cgcatgtatg gtgcaatccc ggcgcatcgg ggcgaatatg
     1921 accgccattt aatgaatact gcccaggctg ccttgctatc tggcaaatcc ataatgatta
     1981 tgcctgaagg aactcgttcg cacgcgccgg gtatgcgtcg tgccgcaccg ggtgtcgctt
     2041 atcttattga gaagacagac gtgcctgttg tcccggttgg cgtggtgggc acaacggatg
     2101 acttttttca gcgagctatg cggggagaaa agccatcatt gtacatgtac atcggtgccc
     2161 ccttccggtt gccactggtg gacggcaaag gagaagatcg tcgccgaagg cgccaggaaa
     2221 atgctgatct gattatggcg catatcgctg cattgttacc gcctgagtac cagggtgtat
     2281 atgccaacac aacccggat
//
LOCUS       DBNHOH010000334         2433 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1429874, whole genome shotgun sequence.
ACCESSION   DBNHOH010000334 DBNHOH010000000
VERSION     DBNHOH010000334.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1429874"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..1351)
                     /locus_tag="AC16V7_05985"
     CDS             complement(176..1351)
                     /locus_tag="AC16V7_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256703.1"
                     /translation="MLLGASWMIVAEKANPIQTRRLEPMGRVLALGLISGLLHLSRAD
                     GITWLAVAVIMTLVRIARNVDQSGNSVEYFSRATRLRELLLTIIALLLGYLIVMGPWM
                     GRNLAVFGTFLSPGGLRALWFTDYNELFSYPSTLLTPQHWWSSGLDKIIEARLYSLGQ
                     NLQTALIVQGEILLTPLLLLGLWHWRRKVSVQFGLLAWSLTLVIMTIVFPFVGWRGGF
                     LHSGAALQPLFWAAAPSGLDIFIEWGRRVRQWNTYQASRVFRVGMVGMTLLLTIFIVQ
                     RRVIGSSVADPIWGRSWSAYIRLEEILIDQRIKPGEIVMVNNAPGYYAANRRPALSIP
                     NGDGETTLEVAHRYQAAYILLDHNHPEGLAKLYENPADQIGLKHLITFEGTHIFAVK"
     gene            complement(1795..>2433)
                     /locus_tag="AC16V7_05990"
     CDS             complement(1795..>2433)
                     /locus_tag="AC16V7_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256704.1"
                     /translation="GITVWAVEGRMSGYLLQSRLFFVVFPPFAVLAGVGFNVLSQLHG
                     AGIRFGRVAAALVLLVYGFSVLDVGRNSLKQNVPQMIFGTISSEQYLEENLGWYSVAM
                     QAIKTLPEDSQVLMLWEPRSLYCLPKCVPDEVIDRWKHDYFTWKNQVAIIHNWRASGY
                     THLLFYRFGANFVRQHDHRYLSSDWDALDSLLAELPELNDFGQTYILYSLIP"
BASE COUNT          749 a          622 c          493 g          569 t
ORIGIN      
        1 gcccattctc catacaatgc cagatttctg gcataagtct ggtgaatcca ggcatcatcc
       61 aaaggaaagc cgatacgata tgttagccaa cttatatata cataccctgc tgaagataca
      121 agtgacaata cccccaagag catgtatatt ctcggatcag gcttccacaa atccttcatt
      181 ttacggcaaa aatgtgcgtc ccttcaaagg taattaagtg cttcaatcca atttgatcag
      241 caggattttc atataatttc gccaaacctt ctgggtgatt atgatctaat aggatatatg
      301 ctgcttgata ccgatgggca acctccaaag tcgtttctcc atcaccattc gggatactta
      361 gggcgggacg ccgattggcg gcataatatc caggggcgtt gttaaccatc acaatttctc
      421 ctggcttaat cctttgatct atcaaaatct cctctaagcg aatataggct gaccaagatc
      481 tgccccaaat ggggtctgcc actgaagacc ctataaccct tctctgaaca atgaaaatgg
      541 tgagtaataa ggtcattcca accataccga cccggaaaac acggcttgcc tgatacgtgt
      601 tccattgtcg cacgcgtctc ccccattcaa taaaaatatc caatccagat ggagcagctg
      661 cccaaaatag aggctggaga gcagcaccag agtgtaaaaa cccaccacgc cagccgacaa
      721 atggaaatac tatcgtcata ataaccaacg ttaacgacca agctaacaag ccaaattgca
      781 cagacacttt tcttcgccaa tgccacaacc caagcaaaag aaggggtgtc aataaaattt
      841 ctccttgaac gatcagggcg gtttgtaaat tttgtcccaa ggaatacaag cgtgcctcga
      901 tgattttatc caacccgctt gaccaccaat gctgtggggt gagcaaagtg gatggataag
      961 agaaaagctc gttgtaatct gtgaaccaca atgcccgcaa tccacccggc gagagaaatg
     1021 ttccaaaaac agccaagtta cgccccatcc aggggcccat aactatgagg tagcctaaca
     1081 acaaggcgat aattgtcaac aataattccc ttaggcgggt tgccctactg aaatattcga
     1141 cactatttcc tgactggtca acatttctgg ctatcctgac caaggtcatt atcactgcaa
     1201 ccgccagcca agttatccca tcggcccgag atagatgaag aaggcctgag atcagtccta
     1261 aggccaaaac tctgcccatc ggttccagac gtcgcgtttg gataggattc gctttctcag
     1321 caactatcat ccaagaagcc cccagcaaca tgtagatccc gaaggtatcc gtcgtcccca
     1381 tataggttag ataaaaccca ggaaaggccg caagaagccc agcaaggatt gcatgatctc
     1441 tctgtccgct aatcgaatat gctagcgaag aagtcacagg tggcacaagg cctgcgataa
     1501 aaattagacc taatctggca gcccaaaagg atgtagatcc tgcaaccttc atccctaacg
     1561 ctgcgatcag cgaggcgagt ggcatccaat atgcatggga tggatgaggc aaaccgtggg
     1621 ggttatccag gtagttccac aacaactctt cggtaaatcc tttgccttct gccagacgaa
     1681 ttccacctgc ataatagtag tccgcatcca tgtaaccggg tgcaatttga aatgtaccca
     1741 ccaataaaat tacggctatg ccgagggtaa aaaggaataa ctgaattcgc cagctcatgg
     1801 tatcaggctg tatagaatgt atgtctgacc aaaatcgttc agttcaggta actcggctaa
     1861 caaagaatca agtgcatccc aatcagaaga caaataacga tggtcatgct ggcggacaaa
     1921 gttcgcacca aatctataaa atagtagatg ggtatatccg ctcgcccgcc agttatgtat
     1981 gatagctact tgattcttcc aagtaaaata atcatgtttc caccgatcaa ttacttcatc
     2041 tggaacgcat tttggtaagc agtacaggct gcgcggttcc cataacatga gcacttgaga
     2101 atcttccggt aatgtcttta tcgcctgcat ggctacagaa taccagccca aattctcctc
     2161 taaatattgc tcagaagaga tcgtgccaaa aatcatttgt ggcacgttct gcttcaaaga
     2221 attcctccca acatctagaa cactaaatcc ataaactaat aagacaagcg cagcggcaac
     2281 acgtccaaat cggatgcctg ccccatgcaa ctggcttaat acattaaatc ccacacctgc
     2341 cagtaccgca aatggtggaa atacaacaaa gaataatctg gattgaagga gataacccga
     2401 catgcggcct tccacagccc agacagttat acc
//
LOCUS       DBNHOH010000335         2675 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1429921, whole genome shotgun sequence.
ACCESSION   DBNHOH010000335 DBNHOH010000000
VERSION     DBNHOH010000335.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1429921"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="AC16V7_05995"
     CDS             complement(<1..322)
                     /locus_tag="AC16V7_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256705.1"
                     /translation="MPEIQIRPAIASDIPAIIAIDHNYSSDYVWQMEIQSEEGQVGVS
                     FRQIRLPRSVRVEYPRPISQLEHDWTQRPGLLVASLNEETVGWIGLRLDIAPVTAWAT
                     DLGVL"
     gene            complement(337..1047)
                     /locus_tag="AC16V7_06000"
     CDS             complement(337..1047)
                     /locus_tag="AC16V7_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256706.1"
                     /translation="MVEALRFFRAYEIWIYLILGLGGLIYIRKFILAWQELREAAFGL
                     ERESALGRLNQSASILVVVLALTITEFFLVSFVAPSLPEAIPLLTPTLDLLATTTSTL
                     PPTTLEAGTSPIQATATVASTPAGLAEGCIPGQVMITSPPSGTEISGVVVISGTASIT
                     NFGFFKLEMKRVEESNWLTILAGNEVKVDDTLGAWNTSLLTPGDHELSLVVTDNQGNS
                     LSPCIIGVRVINTAAPQP"
     gene            complement(1114..1950)
                     /locus_tag="AC16V7_06005"
     CDS             complement(1114..1950)
                     /locus_tag="AC16V7_06005"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIR6256707.1"
                     /translation="MSEFITLEQIDQATNHIRSRITTSPRVGLILGSGMGILAESVEN
                     GTQISANELPNWPVSTVEGHQGRLVIGKLEGKQVMVMQGRSHYYEGYSMGQITLPVRV
                     MQRLGLEILVVTNAAGAVNPSFEPGDLMLITDHLNLVGMTGLNPLRGPNLDEFGPRFP
                     DMSQAYDRQLGKIARQVAVENQIGLQEGVYACLSGPSFETPADLRFLRAAGADAVGMS
                     TVPEVTVARHGGLRVLGISGISNKANLDGSTITTHEEVLESGRVITPKLLAILRGVLR
                     NV"
     gene            complement(2132..>2675)
                     /locus_tag="AC16V7_06010"
     CDS             complement(2132..>2675)
                     /locus_tag="AC16V7_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HIR6256708.1"
                     /translation="LLLHIDGARLFNAVVAQGVDACALAAPADSVTFCLSKGLCAPVG
                     SLLCGSTEFINRAHRMRKQLGGGMRQAGVLAAAGIVALETMVERLAEDHQRARMLAEG
                     LATIHGLVLDPDTPRTNMIFCSLADTVLMNASQVVAALAERGVRVGAVGTRRFRLVTH
                     YWIGDGDVSQAVEAFREVLV"
BASE COUNT          613 a          761 c          708 g          593 t
ORIGIN      
        1 gcaacactcc caggtcggta gcccaagcgg tgaccggggc gatgtccagc ctcaaaccga
       61 tccagcctac cgtctcttca tttagcgagg ccaccaataa tccaggtcgt tgtgtccaat
      121 cgtgttccaa ctggctaatg ggtctgggat attctactcg cacagagcgt ggcaggcgaa
      181 tttggcggaa gctgacaccc acctgtcctt cttcagactg gatttccatc tgccatacat
      241 aatcgctgga gtagttgtga tcaattgcga tgatcgcggg gatatcggaa gctattgctg
      301 ggcgaatttg gatctcaggc atcaatacca cctttattag ggttggggtg ccgcggtgtt
      361 gatcacccgc accccgatta tacaggggga tagagagttc ccttggttgt cagtgaccac
      421 caggctgagt tcgtgatcac ccggggtgag caagctggtg ttccaagctc cgagcgtatc
      481 atctactttg acctcattcc cggccaggat ggtcaaccag ttgctttctt ccacccgttt
      541 catctccagc ttaaagaaac cgaagttggt aatgctggcg gtgccgctga taaccaccac
      601 cccgctaatt tcggtgccgc ttgggggaga cgtaatcatc acctgtccgg ggatgcatcc
      661 ctcggccaat cccgctgggg tggaggctac cgtggcggtt gcctggatag gtgacgtccc
      721 tgcttcgagt gtggttggag ggagtgtgga agtggttgta gccagcaaat ccagtgtagg
      781 ggttagcaag gggatagcct caggaagaga gggagcaaca aaagatacca aaaaaaactc
      841 tgtaatcgta agcgccagca ccaccaccaa tatgctggcc gattggttta ggcgccccag
      901 agcactctcg cgttccaagc caaaagccgc ctcgcgcagc tcttgccagg ccagtataaa
      961 tttacggata tagattagcc cccccaagcc tagaatcagg tagatccata tttcgtaggc
     1021 tcgaaaaaaa cggagcgctt ctaccataaa gaaattcgat acctgggcaa tcttgaagct
     1081 agcggacgcc ggactccaaa ggaagggata aggttatacg ttgcgcagca cccccctgag
     1141 aatggccaat aatttcggag tgatcacacg tccagattcc aatacctctt cgtgtgtggt
     1201 gattgtactg ccatccagat tagccttatt gctaatgccc gagataccca gcacacgcaa
     1261 cccaccatgc cgggcaacag tcacttcagg cacagtagac atgcccactg cgtcagcccc
     1321 ggctgcacgt agaaaacgca aatcggcggg tgtctcgaac gatggaccag acaagcaggc
     1381 gtaaacgcct tcttgcaatc cgatctgatt ttcaaccgcc acctgccggg caatcttgcc
     1441 gagctgacgg tcatatgcct ggctcatatc cgggaagcgt ggtccaaact cgtccaggtt
     1501 agggccgcgc agcggattga gaccggtcat gccaaccagg tttagatgat cagtgatcag
     1561 cattaagtcg cccggttcga agctggggtt gaccgctcca gctgcgttgg tgacaaccag
     1621 tatctccagc cctaggcgct gcatgacgcg cactggcagc gtgatctgcc ccatactata
     1681 gccttcatag tagtgcgacc ggccttgcat gaccatgact tgcttgcctt ctagttttcc
     1741 gatgaccaag cgtccctggt gaccttctac agtggatact ggccagtttg gcaactcatt
     1801 ggcagagatt tgtgttccat tttcaacaga ttctgccagg atgcccatgc ccgagccaag
     1861 gattagccca acgcggggag atgtggtgat acgggagcgg atgtggttgg tggcctgatc
     1921 gatttgttct agtgtgatga attcgctcat agatcgtttc cattgaattg ggatatgacc
     1981 ccaccgcagt tgacaattat aacatgcgta tctcttcgtg ctttcctacc ccgcagggca
     2041 atcgctctaa tgtccccccc attccttcac ggggcgatag gagcaagccc aaaaatctcc
     2101 tatactaggg taaatcagcg aacgctctct attaaaccaa tacctcccta aacgcctcta
     2161 cggcttggga tacgtcccca tccccgatcc agtaatgtgt caccaagcga aagcgccgcg
     2221 ttccaactgc gcccacgcgc acaccgcgct ctgccagggc tgcgacgacc tgggaagcgt
     2281 tcataagcac tgtatcggct aatgagcaaa agatcatatt cgttctgggt gtatcggggt
     2341 ccaataccag accatggatg gtggctagcc cttcggctaa cattctggcg cgttggtggt
     2401 cttcagccag gcgctcaacc atggtctcta aagcgacaat gccagctgcc gccaggacac
     2461 ctgcctggcg catccccccg cctaactgct tgcgcatgcg gtgcgctcgg ttgatgaact
     2521 ccgtggagcc gcacaacaac gagcccaccg gtgcgcacaa ccctttgctc aggcaaaagg
     2581 tcaccgaatc ggctggcgca gccagggcgc aggcatctac cccctgggca acaaccgcgt
     2641 tgaataggcg cgctccatca atgtgcagga gcagg
//
LOCUS       DBNHOH010000336         2150 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_724696, whole genome shotgun sequence.
ACCESSION   DBNHOH010000336 DBNHOH010000000
VERSION     DBNHOH010000336.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_724696"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="AC16V7_06015"
     CDS             complement(<1..523)
                     /locus_tag="AC16V7_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HIR6256709.1"
                     /translation="MGYRPFTSKRAPIYADVPDDKWNSWRWQLSNRINTVEEFEKVIR
                     LTDGERQALSTPNLFRVDITPYFISLIDPDDPNDPIRKQVVPSSAELVPFTSMMEDSL
                     AEDRHSPVPGLVHRYPDRVLMLVTTQCASYCRYCTRSRIVGDPSATFSRVEFEMQLEY
                     LKNTPQVRDVLLSG"
     gene            complement(613..1632)
                     /locus_tag="AC16V7_06020"
     CDS             complement(613..1632)
                     /locus_tag="AC16V7_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256710.1"
                     /translation="MASSPRQPLRWIFIFGVLLAGCDSLSQPALPTALPLEYIPTAVA
                     LTAGASGASLHASTQAATPSAQDMLLGTPTPETHSLPTASATPNPSSTPTLSPTGPSP
                     TPYTLPPSPTATPTPEIPNAEIEIRNLGPLSRVTSPLHVYLYLKPGAGGKVLIELLGE
                     ENRVLYREIRKINFVPVGAWAVFTVDFDFEIPGVAEAGRLKISVDDEFGRTVALNSVP
                     LILLSVGDADIIPPPDVLAPIVIRQPRRKALVQGGKLVVSGQVRSSDDQALMVKLITT
                     TGSEVGFRLAEVDAPAEGDFATFAVEVSYTVSEPTPALLVVQQGAGTLNDIAHLSSIE
                     VLLSP"
     gene            complement(1632..1997)
                     /locus_tag="AC16V7_06025"
     CDS             complement(1632..1997)
                     /locus_tag="AC16V7_06025"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6256711.1"
                     /translation="MARLLFVDDDPFTLETLAKAVQVLGHQPILASNGQEALLAAEEQ
                     SPDLIFTDMSLPDTDGATLIELLHGQESTAKIPMFILSASPTEDAIERTRSAGAQAYL
                     NKPIRLQTLIDIIGKFAVG"
BASE COUNT          520 a          570 c          615 g          445 t
ORIGIN      
        1 cgccagagag caacacatcg cgcacctgag gtgtattctt caaatactcg agctgcattt
       61 caaactcgac gcgcgagaac gttgccgatg ggtcacccac gatgcgcgag cgcgtacagt
      121 agcggcaata ggaggcgcac tgagtagtaa ccagcatcaa cacccggtct gggtaacggt
      181 ggactaatcc aggaacgggc gagtgacgat cctccgccag cgagtcctcc atcatggatg
      241 tgaacggcac tagctcggct gagctaggca ctacctgctt gcggatagga tcgtttggat
      301 cgtccgggtc aatcaacgag atgaaatagg gggtgatatc cacccggaac aagttggggg
      361 ttgaaagagc ttggcgctcg ccatcggtga ggcggattac cttttcaaat tcttcaaccg
      421 tgttaatgcg gttactcagt tgccaacgcc agctattcca cttatcatcg ggcacgtcag
      481 cgtaaatggg tgcacgtttt gaggtgaatg gtcgatatcc catacaaaat actcctaatc
      541 gaggttacca aaagcctgaa tatagtttag cccaatctcg cgtagatgaa aaggtgtacc
      601 gcccgaatca gcttatgggc ttagcaaaac ctcgatgctg gacaggtggg cgatatcgtt
      661 taatgtgccc gcgccctgct ggacgaccaa taacgccggg gtcggctcgc tgactgtata
      721 ggagacctct accgcaaatg tggcgaaatc gccttcggca ggcgcatcca cctctgccag
      781 acggaagcca acttcactcc cagtggtggt gatcaatttc accatcagcg cttgatcgtc
      841 actggagcga acctgcccag aaacaaccag cttacctccc tggaccaggg ctttcctccg
      901 cggctgccgg atgacaatcg gggcgagcac atctggcggc gggatgatat ccgcatcgcc
      961 aaccgagagc aagatgagcg gaacggagtt aagcgccact gtgcgcccga actcatcgtc
     1021 cacgctgatc ttcaagcgcc ccgcctcagc caccccaggg atctcgaaat caaaatccac
     1081 cgtgaacacc gcccaagcgc ccacagggac aaaattgatc ttacggatct cgcgatataa
     1141 aacgcggttc tcttcaccca acagctcgat gagcacctta cctcccgcgc ctggtttgag
     1201 atacagatag acatgcagcg gagacgtgac ccgagaaagc ggtccgaggt tgcgaatttc
     1261 gatctcagcg ttgggtatct ccggcgtagg agtagcagta ggagagggcg gcaaggtgta
     1321 aggggttgga cttggccccg tggggctaag tgtgggggtc gaagacgggt ttggcgttgc
     1381 acttgcagta ggaaggctgt gtgtttctgg ggttggcgta cctagcagca tatcctgggc
     1441 tgacggggtc gctgcctggg ttgacgcatg caaagatgcc ccactcgccc ccgctgtgag
     1501 tgctaccgca gtaggtatat attcaagggg cagtgcagtg ggcaaagccg gctgtgacag
     1561 gctatcgcaa ccggctaaaa gaaccccaaa tataaaaatc caacgcaaag gctgacgtgg
     1621 ggaagacgcc atcaacccac cgcgaatttc ccgatgatgt cgatcagagt ctgcaggcgg
     1681 atgggcttat tgagatacgc ctgggctccg gcagatcggg ttctttcgat cgcgtcctcg
     1741 gtgggactgg cagaaaggat gaacataggg attttggccg tactctcctg tccatgcaac
     1801 agctcaatca atgtcgcgcc gtcggtgtcc ggcaagctca tgtcagtgaa gatcaggtct
     1861 ggagattgtt cttctgcggc caacagcgct tcctggccgt tgctggcaag gatgggctga
     1921 tgcccaagca cttgaacagc cttggccaat gtttctaggg taaaaggatc atcatctaca
     1981 aaaagtaagc gggccatgaa ttccccttga ggacctataa taagatttta ccacagcatg
     2041 tggcgtggcg ataggtgatc gttccaaagc tggtagaaac gtggttaaat ggttgagggg
     2101 cagttagcgg accacccccc aacctacaaa cagaaaggca cacgtgcatg
//
LOCUS       DBNHOH010000337         8417 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_917204, whole genome shotgun sequence.
ACCESSION   DBNHOH010000337 DBNHOH010000000
VERSION     DBNHOH010000337.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8417)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8417)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8417
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_917204"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="AC16V7_06030"
     CDS             complement(<1..768)
                     /locus_tag="AC16V7_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012299.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6256712.1"
                     /translation="MSAMENLPLVETDNSPPSAQPSNGAKSALIIGAGIAGIQAALDI
                     ADAGFKVYLVERSSSIGGRMSQLDKTFPTLDCSACILTPKMADISRNPNITLLTGTQV
                     VGVEGQAGTFHVQLEQQPRYVDVTSCTGCGLCADACPVEMPSEFNAGLVTHKAIYRRF
                     PQAVPAAYVIEKRESPCKSACPAHIPVQGYTALIAQGKFREALERIRQAGVAFPGTLG
                     RVCYHPCESICKRGEWDEPVAICALKRFAYDAAADDDT"
     gene            complement(768..1880)
                     /locus_tag="AC16V7_06035"
     CDS             complement(768..1880)
                     /locus_tag="AC16V7_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR6256713.1"
                     /translation="MKTIIAVENSGVLAGVRHFLKRLMETGVVEAIYVPMQVDGGTII
                     PALVTDPRWLDQADPLSPVMPINGARAVSALTGKHSPGRLGAVLRSCEIRALLELVKL
                     QQASLDGMSLISVDCPGTCELKDYEAMKRNGGFDLESFLESASAGSEPQGFNLRAACQ
                     MCLQPDYEAADAHLQLFGADVQQGIPVVLRDDIAMKLELVADPNPSDGRKQIIDQVVT
                     RHEQKRQAELSAIRGELSSNGGMASLFSTCLRCHNCMTACPICYCKTCLFRSEAMSHP
                     PEYYLNAAHRKGAVRLLSDTLLFHMTRLNHMGTSCVSCGMCASACPADIPVGTIFSAI
                     SEQVQTAFGYQPGRDVGEPLPLITFQPNEWTEIGEG"
     gene            complement(1933..2361)
                     /locus_tag="AC16V7_06040"
     CDS             complement(1933..2361)
                     /locus_tag="AC16V7_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HIR6256714.1"
                     /translation="MNNDKFEPHIVAFLCNWCTYTGADLAGTSRLQYPSNIRIVRTMC
                     SGAVDPVYVLKLLMDGADGVLVGGCHPGDCHYQSGNYKARRRYVALREILKAAGFDEQ
                     RVWLRWISASEGQKFADTVTEMVSALTELGPNPTREIWAV"
     gene            complement(2377..7005)
                     /locus_tag="AC16V7_06045"
     CDS             complement(2377..7005)
                     /locus_tag="AC16V7_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6256715.1"
                     /translation="MLSSDNGNHNTKKPVGAALVVGAGIAGMQAALDLAESGIKVYLA
                     ERGPAIGGTMAQLDKTFPTNDCAMCIMSPKLVEVGRHLNIDLITCAEVDGISGEPGDF
                     VVSMRQRPRYVDSLKCTGCGDCATVCPISLPDAFNGGLSTRKAIFKLYPQAIPNAFSI
                     DKRGTAPCRDACPIHQRAQGYVALVSEGRYADAYRTIREDNPFPSICGRVCNHRCEDA
                     CNRAQADAPVNIMALKRFVADWAWENLLDTPITGSPSVEGDGETGVSFPEARKTRGEG
                     ERPLSGRRVAVVGSGPAGLTCAQDLSGDGCEVTIFEALPVPGGMLRAGVPVYRLPPEV
                     IRREIDQIINNGIDLQLNHRIEDVESLLGEFEAVFVAIGAHAGVKLPIPGNDLPEVWM
                     ATDFLRQANLAISGDLQPGESALLHTQVQDQRVLVLGGGNVAIDAAMTAVRLGAKWVG
                     MTCLEGREQMPAHAWEVRDAEEEGIAVYPSRTFKEVTSSEGRCTGVRTVEVNFRGFID
                     GRPDFDEIPGTEQVIPADIVIFAIGQRPESDCLKRVKLLRGGRVSVDAETLATDVPGI
                     FAGGDAVTGTAFVVDAIAAGHRAARSIGAFLLGCEDLKLDRNQANVVKLDSHEVSQRI
                     AIGDASSAIRAETKKRPAAERKLDFNEIYAGLSEDEAKAEAMRCLRCGVCSECNQCVY
                     ACRAEAILHEDAERLTELNVGAVILAPGVEAMPGDIRPEYGYGRYPNVVTSVQFERML
                     SASGPFGGVVQRPSDGKHPLKVAWIQCVGSRDYTSLPDGTPRAPYCSSVCCMYATKEA
                     IIAKEHDPRIQPTIFYMDVRSFGKGFEGYIERARGEHGIRYIRCMVSSVSEAPGTKNL
                     RLGYVTYEGSNGRKPNLHEEEFDLVVLCVGLRPSPAARATAERLGVELNEYGFVAGQA
                     FEPSLTSCPGIYAAGAFAEPKDIPESVIEASCAAAQASALLGEARASQTRLPSFPPER
                     DISDEPEKVGVFICHCGINIGSVVDVPAVVEHAMTLPGVAYAEHNLYTCSQDTQERIR
                     QRIQENGLNRVVVSSCTPRTHEPLFQDTLRAAGLNPHLFEMANIREQDSWVHRSKPQA
                     ATEKAEQLVSMAVAKARRLRAIRRDSFAVESRALVVGGGLAGMTAALSIARQGYDVFL
                     VERSPQLGGKLRYLYTSAPGGGDPQVLLQETIRAVKTEPRIRVFTETVLTELRGYTGQ
                     YTSTVQQSDGSTLELQHGAVVVATGGQPYKPREYLYGKHSRILTQLELEDLLTHPANH
                     MPQVIQSGQSFVMIQCVGSRDDEHPYCSRVCCTQALKNALVIKEKFPDARVTVLYRDI
                     RTYGFREQLYRQARQAGVVFLEYSEADKPTVTPDGERLRIEVAIQPENEAVNLAADWL
                     VLSAGIEPEPDNPSLAQILKVPLNEAGYFLEAHVKLRPVDFAAEGIFLAGLAHSPRQI
                     EETIAQAHAAAVRAVGLLSRSRLEATPIIASVNPKLCAACGLCVEICPYGARLLEPGM
                     SHAEVIDVLCQGCGACVVACPNKASQQKGFEFAQVFGMVEEALNLPHS"
     gene            complement(7022..7918)
                     /locus_tag="AC16V7_06050"
     CDS             complement(7022..7918)
                     /locus_tag="AC16V7_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HIR6256716.1"
                     /translation="MESAFSPKQVQKEKRPVYSYYPGCSLHSTGLEYGQSTREVFRAL
                     EFELAELDGWNCCGASSAHALNHTLAYALPARTLALAQAMGRELVTPCAACFNRLKQA
                     DHALKSEPELRAEIESVVGFQYSGEVAIRPVLAVLFEDVPASRLVELVKFPLNGLKVV
                     PYYGCLLVRPPQVTRFDDPDHPQVMTKLLCSIGAEVMDWSQATDCCGAGLSLGRPDVV
                     QGLVGRLVERAREAGAQALVTACPLCQMNLEMRQTGPDKMPAIYFTELLGLAFGLPEA
                     KKWWPKHLIDPRPVLRMAGVSV"
     gene            complement(7919..>8417)
                     /locus_tag="AC16V7_06055"
     CDS             complement(7919..>8417)
                     /locus_tag="AC16V7_06055"
                     /inference="COORDINATES: protein motif:HMM:NF024581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR6256717.1"
                     /translation="KRLSNQQVELCYHCHKCAAGCPVVGAMAYGPDRILRMAALDQYE
                     SIFQSKDIWLCAGCYTCATRCPNSIDIAAVMDSLRQLALAEGYQAGEPDVFVFHRLFL
                     GVLQRLGRSHEAILLGLFKLRSHQPLMNDLGAGFGLVLRGKIPVLPQNVKAAPPVRKI
                     FQGTK"
BASE COUNT         1907 a         2544 c         2254 g         1712 t
ORIGIN      
        1 agtatcatcg tctgcagccg cgtcgtatgc aaagcgtttg agggcacaaa tcgccacagg
       61 ctcgtcccac tcaccccgct tgcagatact ctcgcaaggg tgatagcaca cgcgtcccaa
      121 ggtgccaggg aaagccaccc cggcctggcg gatgcgctct aacgcctcac ggaattttcc
      181 ctgcgcaatc aaagcggtat agccttggac cgggatgtgc gccgggcaag cactcttaca
      241 aggagactcc cgcttctcga tgacataggc tgcgggtaca gcctgaggga aacggcggta
      301 gatcgccttg tgcgtcacca gccccgcatt aaattcgctt ggcatctcca ccgggcaggc
      361 gtcggcgcac aggccacagc cggtgcaact cgtcacatct acataacggg gctgctgctc
      421 caactgaacg tggaatgtcc ctgcctggcc ttctacgcca accacctgcg tgccggtcag
      481 cagggttata ttgggattgc gcgagatgtc agccatcttg ggcgttagta tacatgcgga
      541 gcagtccagc gtcggaaaag ttttgtcaag ctgggacatg cgcccgccaa tgcttgacga
      601 gcgctcaacc aggtacactt tgaagcctgc atcagcgata tccagcgccg cctggattcc
      661 cgcaatcccc gcgccgatga tcagcgcaga tttggcacca ttcgagggtt gggcagatgg
      721 tggagaatta tctgtctcga caagaggcaa gttttccatt gcgctcatca cccctcacct
      781 atctctgtcc actcattcgg ctggaatgtg atcaatggca gtggctcgcc tacatccctg
      841 ccaggctggt agccgaaagc tgtctgcacc tgctcgctga tggcgctaaa tatggtcccc
      901 accgggatgt cggccggaca ggccgacgcg cacatgccac agctgacaca tgaagtaccc
      961 atatggttaa ggcgtgtcat gtgaaacagc agggtatcgc tgagcaggcg aacagccccc
     1021 ttgcgatgcg cagcgttcag gtagtattcc ggcgggtggc tcatcgcctc cgagcggaac
     1081 aggcaggtct tgcaatagca gatcgggcag gcagtcatac aattgtggca gcgcaagcag
     1141 gtggagaaca aggacgccat gccgccgttg gaagagagct cacctcgaat ggctgagagt
     1201 tcagcttgcc ttttctgctc atgcctggtc accacctgat ctataatttg cttgcgtcca
     1261 tccgaaggat tggggtcggc aactagctca agtttcattg cgatatcatc ccgcagcacc
     1321 accggtatgc cctgttgcac atccgcgcca aagagttgca agtgagcatc cgccgcctcg
     1381 tagtctggct ggaggcacat ctggcaggcg gcacgcaggt taaaaccttg tggttccgat
     1441 ccggcagagg cactttccag gaaactctcc aggtcgaagc caccgttacg cttcatggcc
     1501 tcataatcct tcaactcaca ggtgccagga caatccactg agatcaggct cataccatcc
     1561 agtgaagctt gctgcagctt taccaattcc agcagggcac ggatttcgca tgaccgcagg
     1621 acagctccca ggcgcccggg tgagtgcttg ccggttagcg cagaaacggc gcgcgctccg
     1681 ttgatgggca tgacgggtga tagtgggtcg gcttggtcta gccagcgcgg atcagttaca
     1741 agagcaggta tgatggtccc gccatcgact tgcataggaa catagatcgc ttccaccacg
     1801 ccggtctcca tcaaacgctt caggaagtgc ctaaccccag ctaacacccc gctattttct
     1861 actgcgatga ttgttttcat atatacctcg ctcgttagga cgcagagcgt aatcagtacc
     1921 gccgtttaat gcctaaactg cccatatttc ccgagtaggg tttgggccca gctcagtgag
     1981 agcagatacc atctccgtca ccgtatcagc aaatttctgt ccttcactgg cgctgatcca
     2041 ccgcagccag acacgttgct catcgaatcc agcagctttc aagatctccc gcaaagcaac
     2101 gtagcggcgc cgcgctttgt aattgcccga ttggtagtgg caatcgccag gatggcagcc
     2161 accgaccagc acgccatctg caccatccat caagagcttc agcacataga cgggatcgac
     2221 cgccccgctg cacatcgtgc ggactatgcg gatgttggac gggtactgca agcgcgaagt
     2281 acccgccagg tctgcgccgg tataggtaca ccagttgcac aggaatgcta caatgtgcgg
     2341 ttcaaatttg tcgttgttca taatgcctcg tattaattag gaatggggaa ggttgagcgc
     2401 ttcttctacc atgccaaaca cctgggcaaa ctcaaagcct ttctgttggc tggctttatt
     2461 ggggcaggcg acgacacagg cgccacagcc ctggcataaa acatcgatga cttcggcatg
     2521 gctcatgccg ggctccaaaa ggcgcgcacc gtaagggcat atctccacac acaacccgca
     2581 ggcggcgcac aatttagggt taacgctggc aataatcggt gtggcctcca gccgcgaacg
     2641 ggagagcaga cctaccgctc gcaccgcagc agcatgggct tgggcgatag tctcctcgat
     2701 ctgacgaggc gagtgagcca ggccagccag gaagattccc tcagcagcaa aatctaccgg
     2761 gcgcaacttg acgtgagcct ccaggaagta tcccgcttcg tttagcggca ctttcaaaat
     2821 ttgggccagg gaggggttat ccggctcggg ttcaatcccc gcgctgagca ccagccagtc
     2881 ggcagccagg ttgaccgctt cattttccgg ctggattgcg acctcgattc gtaatctctc
     2941 accatctgga gtgacggtgg gtttatccgc ctcgctgtat tccaggaaga ccacgccagc
     3001 ctgacgcgcc tgccggtaca attgctcgcg gaagccataa gtgcgaatgt cgcgatagag
     3061 caccgtcacg cgcgcatcag ggaatttttc ttttatcacc agtgcattct taagggcttg
     3121 agtgcaacag acgcgtgagc aataaggatg ctcatcatcg cgcgagccta cgcactgaat
     3181 catgacaaac gattgtcccg attggatgac ttgaggcatg tgattcgctg gatgagttag
     3241 caggtcttcc agctccaatt gggtcaaaat gcgtgagtgc ttgccgtaaa gatactcacg
     3301 cggcttgtac ggttgaccgc ccgtagccac taccaccgca ccatgctgca gctcaagggt
     3361 tgaaccatcc gattgctgca ctgtgctggt atattgaccg gtataaccgc gcagttcggt
     3421 taatactgtc tcggtgaata cacggattcg tggctctgtc ttcaccgccc ggatcgtctc
     3481 ctgaaggaga acctgcggat cccctccacc gggcgcactg gtgtacaagt agcgcagttt
     3541 tcctcctaac tgcgggctac gctcaaccaa gaacacgtcg taaccctggc gagcgatgga
     3601 taatgctgcc gtcatccctg ccaggcctcc gccgaccacc agggcccggc tttctactgc
     3661 gaagctatcg cgccggatgg ctcgtaagcg gcgtgctttg gcgacagcca tcgataccaa
     3721 ttgttcggct ttttccgtgg cagcttgcgg cttggagcgg tgcacccagg aatcttgctc
     3781 gcggatattg gccatctcga acaaatgcgg gttgagtcca gccgcccgta gcgtatcttg
     3841 gaagagcggc tcatgggtgc gcggcgtgca agaagacaca accacacggt tcaaaccatt
     3901 ttcctggatg cgctggcgaa tgcgctcctg ggtatcttgc gagcaagtgt ataggttatg
     3961 ctcggcatag gccaccccag gcaaggtcat ggcatgctca accacggcgg gcacatctac
     4021 cacagagcca atattgatgc cgcaatggca gatgaacact cccaccttct cgggctcgtc
     4081 ggaaatatct cgctcggggg ggaaagaagg caagcgggtc tgcgaggcgc gcgcctcacc
     4141 caacagcgct gaggcctggg cagccgcaca ggaggcttct atcaccgatt cggggatatc
     4201 ctttggttcg gcaaacgcac ccgcggcata gattcctggg caagaagtga gactcggctc
     4261 aaaagcttga ccagcgacaa agccgtactc atttagctca acccccaggc gttctgcggt
     4321 tgcacgcgcc gcaggtgaag gtctcaaacc cacacacaga acgaccagat caaattcttc
     4381 ctcatgcaga ttaggcttgc gcccattgct tccctcgtaa gttacatagc caaggcgcaa
     4441 atttttcgtc ccaggcgctt cgcttaccga cgagaccata catcgtatgt agcggatccc
     4501 gtgttcacca cgtgcgcgct cgatatagcc ctcgaaccct ttgccaaagg agcgcacatc
     4561 catgtagaaa atggttggct gaatacgggg gtcatgctcc ttggcgatga tggcttcttt
     4621 cgtcgcgtac atacagcaca ccgacgagca atatggcgcg cggggggtgc catccggtag
     4681 gctggtgtaa tcgcgcgagc ctacacattg tatccaggct acttttaggg gatgtttgcc
     4741 atccgaaggg cgctgcacca caccaccgaa gggtccagag gcggagagca tgcgctcgaa
     4801 ctgcacactg gtgacgacgt tgggatagcg cccataacca tactccgggc gaatgtcgcc
     4861 cggcatagcc tccacacctg gagccaagat gaccgcaccg acattcaact cagtcagccg
     4921 ctcggcatct tcatgcagaa ttgcctctgc gcggcaggca taaacacact ggttgcactc
     4981 tgagcaaaca ccacagcgca ggcagcgcat cgcctccgcc ttggcctcgt cctcgcttaa
     5041 gccagcgtat atctcgttaa aatccagctt gcgctcggcc gcagggcgct tcttagtttc
     5101 agcgcggatt gcgctggatg catctccaat ggcaatgcgc tggctgactt catgcgagtc
     5161 gagcttaaca acattggctt gattccgatc aagtttcaga tcctcacagc cgagtagaaa
     5221 ggcaccaatg gagcgggctg cgcggtgccc ggccgcgatg gcatccacca cgaaagcggt
     5281 gccggtgacc gcatccccac cagcgaagat ccccggcaca tcggttgcca gcgtctccgc
     5341 gtccacagac acccgcccgc cacgaaggag cttgactcgc ttcaggcaat ccgattcggg
     5401 tcgctgtcca atggcaaaaa tcacaatatc tgccggtatg acctgctcgg tgccgggaat
     5461 ctcatcaaaa tcaggccgcc catcgataaa cccgcggaag ttgacttcaa ccgtgcgcac
     5521 acctgtacat cgaccctctg aactggtcac ttctttgaac gtgcgggatg ggtagaccgc
     5581 gatcccctct tcctctgcgt cccgcacctc ccaggcgtga gcaggcatct gctcgcgccc
     5641 ttccaggcaa gtcatcccaa cccatttggc tcccaggcgt actgccgtca tggctgcatc
     5701 gatggccaca ttaccgcctc ccaggacgag cacccgctgg tcttgtacct gggtgtgcag
     5761 cagcgcactt tcgcccggct gtagatcgcc gctgatagcc aggttggcct ggcgcagaaa
     5821 atcggtcgcc atccatactt caggcagatc gttgcccggt atgggcaact tcacgccggc
     5881 gtgcgcaccg atggcgacaa agaccgcctc gaactcgcct aaaagcgatt cgacatcttc
     5941 aatccgatgg ttgagttgaa gatcaatccc gttattgatg atctgatcga tctcccggcg
     6001 gattacctca gggggcaagc ggtagacagg caccccagcg cgcagcatac cgcccggcac
     6061 aggtagcgcc tcaaagatgg ttacttcgca accatctccc gacaaatcct gagcacaggt
     6121 caaccctgcc gggcctgatc ccaccaccgc caccctgcgg cctgacaagg ggcgctcgcc
     6181 ctcgcccctt gtctttctgg cctcaggaaa ggacacgccc gtttctccat ctccttcgac
     6241 ggagggggag cctgtgatgg gagtgtccaa taaattttcc caggcccaat cggccacaaa
     6301 gcgcttaagc gccataatat ttaccggggc atccgcctgg gcgcggttgc aggcatcctc
     6361 acagcgatga ttgcacactc gcccgcaaat cgaggggaag gggttatcct cacggatggt
     6421 tcggtaggcg tcagcatagc gtccttccga gaccaaagca acatagcctt gcgctcgctg
     6481 gtgaatcggg caggcatccc ggcagggggc agttccacgc ttgtcaatgg aaaatgcgtt
     6541 agggatagct tgcggataaa gcttaaaaat ggcttttcga gtggagagcc caccgttgaa
     6601 cgcatcaggc aagctgatag ggcaaaccgt agcgcaatct ccacagccag tgcatttcaa
     6661 actgtcgaca tagcgcggcc tctggcgcat gctgacgacg aagtcacccg gctcaccgct
     6721 gataccatca acctcagcac aggtgattaa atcgatgttg agatgccgtc caacttcaac
     6781 cagcttggga gacatgatgc acatggcaca atcgttggtt gggaaagtct tatcgagctg
     6841 cgccatggta ccgccaattg ctggcccgcg ttcagccagg taaaccttaa tacctgactc
     6901 ggccaaatca agggcagcct gcattccagc gatgcccgcc cccaccacca gggcagcccc
     6961 aacgggtttt ttcgtattgt gatttccatt atccgaagag agcataatcc caatctccac
     7021 cttagacaga cacgcccgcc atgcgcaaaa ctggccgggg atcaatcagg tgcttaggcc
     7081 accatttttt ggcttcaggc aacccaaacg ccagcccaag caactccgta aaataaatcg
     7141 caggcatctt atccgggcca gtttgccgca tctccaggtt catctggcat aatggacagg
     7201 cagtcaccag cgcctgagct ccagcttcac gagcgcgctc aaccagacgc cccaccaacc
     7261 cctgaacaac atccgggcga cctaatgaaa gcccagcacc gcagcaatct gtggcttgag
     7321 accagtccat cacctccgcc ccgattgagc agagcaactt tgtcatgacc tggggatgat
     7381 cggggtcgtc gaaccgggtc acttgagggg ggcgcacgag caagcagcca tagtagggaa
     7441 ccactttcaa tccattcagg ggaaatttca ccaactcaac aaggcgactg gcaggtacat
     7501 cttcaaaaag caccgccagc accggtctga tagccacctc cccagaatat tgaaacccca
     7561 ccacagactc gatctcagcg cgcaattcgg gctcgctctt gagcgcatga tcggcttgtt
     7621 tgaggcgatt gaaacaagcc gcacaaggtg tcacgagttc gcgtcccatc gcctgcgcta
     7681 aggccaatgt gcgggcgggc aaggcatatg ccagcgtgtg gttcagggcg tgtgccgacg
     7741 aagcaccaca acagttccag ccgtccaatt cagccagctc aaattccagc gcacggaaga
     7801 cctcacgggt ggattggcca tactccagac cagtcgaatg caaactacag ccgggatagt
     7861 aagaataaac cggacgcttt tctttctgaa cttgctttgg agagaatgcg ctttccactt
     7921 atttagtccc ttggaagatt ttacgaaccg gaggcgcagc cttgacattc tgtggcagca
     7981 cggggatttt ccccctcagt acgagaccga accccgcacc taaatcgttc atcaatggct
     8041 ggtgagagcg cagcttgaag agacccagca ggatcgcctc atgggagcgc ccaagacgct
     8101 gaagcacacc taaaaacagg cgatgaaaaa caaatacatc cggctcacct gcctgataac
     8161 cttcagccaa ggcaagctgg cgtagcgagt ccatcaccgc ggcaatgtcg atactatttg
     8221 gacagcgtgt ggcacaggtg tagcaacccg cacacagcca gatatcctta ctctggaaga
     8281 tcgactcgta ctgatccaag gctgccatac gcaggatacg atccgggcca taagccatcg
     8341 cgccgaccac cgggcagcca gctgcgcact tatgacagtg atagcagagc tcaacttgct
     8401 ggttcgacaa acgctta
//
LOCUS       DBNHOH010000338         3360 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_83125, whole genome shotgun sequence.
ACCESSION   DBNHOH010000338 DBNHOH010000000
VERSION     DBNHOH010000338.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_83125"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /gene="xseA"
                     /locus_tag="AC16V7_06060"
     CDS             <1..818
                     /gene="xseA"
                     /locus_tag="AC16V7_06060"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR6256718.1"
                     /translation="RKRPLPKWPQRIGIVTSPSGAAFRDILNTIRRRYPLPVVILAPT
                     PVQGVEAPGGIVKALHELNRLAHPDVIILARGGGSIEDLWAFNDEDVARAIVASEAPV
                     VTGVGHETDFTIADFAADVRAPTPTAAAEIVTPNRIDLIADLTEFEGRINRALSASIN
                     SRRWALIDQSNRLRLHSPLTRLRSDRQRVDEQARRAWSSLFHRLQLSRAGLGSLEQRL
                     EVLNPMSVLGRGFAVISHPDGELVRSVKQVSSGDTLNVRLVDGDFGVRVDGEV"
     gene            827..1258
                     /locus_tag="AC16V7_06065"
     CDS             827..1258
                     /locus_tag="AC16V7_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256719.1"
                     /translation="MVDEALRPEPPEELPKLAERLNENLRLSGSSSAEHTFGLGCSLG
                     FTPLLVILVILLVLRVINLILAGILLVIGLLALVGVVMLLAQKARYNGIHRAYHTSVE
                     AEINQYLAQNKLSRRQFDVIVSQLLPENAPLQAFLSKQEYV"
     gene            1255..1512
                     /locus_tag="AC16V7_06070"
     CDS             1255..1512
                     /locus_tag="AC16V7_06070"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008562988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIR6256720.1"
                     /translation="MTKSLSNVPIPETPVDELSYEQALAELEGIVAGLETGDHALEAS
                     LALYERGQLLVRYCEKLLEKAELKIQSLSGEDLVDFGQQSD"
     gene            1515..1961
                     /locus_tag="AC16V7_06075"
     CDS             1515..1961
                     /locus_tag="AC16V7_06075"
                     /inference="COORDINATES: protein motif:HMM:NF014711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HIR6256721.1"
                     /translation="MADLFSNYALIAGLVAWGIAQVVKVPLEYLQTRTWNWVLLLRAG
                     GMPSSHSALVTAISYATGLFIGFDQPVFALSVAVAMVVVYDATGIRRQAGKHAALINA
                     MINDLASGHPLKEEQLREVLGHTPLEAIGGVVLGLVTAQLVWFIWG"
     gene            complement(1948..>3360)
                     /gene="murJ"
                     /locus_tag="AC16V7_06080"
     CDS             complement(1948..>3360)
                     /gene="murJ"
                     /locus_tag="AC16V7_06080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HIR6256722.1"
                     /translation="VAGGALASAFIPTFTGILTQEDKKGAWRLASSIFNLILFTMVGA
                     IVLAALFAPQITRSVLAPGFASEPAKEALTISLMRMMLPSAAIFGVSGLIMGILNAHQ
                     VFFIPALTPSMYQLGLIFGVLVLTPKMGIYGLAWGVLIGAGLHLGLQIPALLRQKGQY
                     FPTLGIEYAPVREVARLLGPRLLGVAVVQLNFWVNTRLASLQPEGSVTAIVTAFALML
                     MPQAAIAQSVAIAAMPTLAAQYARGQLREMRRSLADSLRGVLLLSIPASLGLMLLRQP
                     VITLLYQRGAFDSRSTELVAWALLWYAAGLVGHSLVEVLARAFYALHDTRTPVLVGAL
                     AMALNVIFSLLFSALFSRIGWLPHGGLALANTLATALEAVGLVYYMRRRLEGMQGDFI
                     LRGSLQAAAGALVMSAGIWIWLAWAGESPAWRTTLGGLVIGGGIYSVVMWVMGVKEVK
                     GMVAMISGYIARNRKNFTPR"
BASE COUNT          724 a          955 c          954 g          727 t
ORIGIN      
        1 aacgcaagcg ccccttgcct aaatggccgc aacgtatcgg gatagtcacc tcgccgagcg
       61 gtgcggcatt tcgggatatc ctgaacacca tccggcgccg ctatcccctg ccggttgtca
      121 tcctggctcc tacgccggtc cagggtgttg aagcaccagg gggcattgtg aaggcactgc
      181 atgagctcaa ccgcctggca catcccgatg tgatcatcct ggcgcgcggc ggcggctcta
      241 tcgaagacct gtgggcattt aacgatgagg acgttgcgcg cgccattgtc gcctcggaag
      301 cacccgtggt taccggtgtg ggacatgaga ccgacttcac tattgccgat tttgccgctg
      361 acgtgcgtgc cccaaccccc acagctgctg ctgagattgt cacacctaat cgtattgatc
      421 tgattgccga cttgaccgaa ttcgaggggc ggattaatcg tgcattatcg gcgagtataa
      481 attctcggcg ctgggctctc attgaccaat ccaatcgctt gcgcttgcat tcccccctta
      541 cccgcctgcg tagcgaccga cagcgagtgg acgagcaggc gcgccgcgcc tggtcatcct
      601 tgttccaccg cctgcaactg agccgtgccg gcttgggtag tctggagcaa cgcctggaag
      661 tgctcaaccc gatgtctgta ttggggcgcg gttttgctgt gatctcacac ccagatggcg
      721 agctggtgcg cagcgtcaaa caggtcagtt ccggcgatac tttgaacgtg cgcctggtgg
      781 atggcgactt tggagttcgg gttgatggag aggtctaggg gtatttatgg tagatgaagc
      841 attaaggcca gagccacctg aggagctgcc caagctggct gagcgcctga acgaaaacct
      901 gcggctcagc ggatcaagca gcgctgagca tacttttggg ctgggatgct cattgggatt
      961 tacccctctc cttgtgattc tcgtcatctt gcttgtcctg agagtgatca atttgattct
     1021 ggcgggcatc ttgcttgtga taggtttgct agcactggtc ggggttgtca tgttgcttgc
     1081 ccagaaagcg cgttataacg gtatccaccg ggcataccac accagtgtgg aagcggagat
     1141 taaccaatac cttgcccaaa acaagctgtc ccgccgccag tttgatgtta tcgtctcaca
     1201 acttttacct gaaaacgccc cccttcaggc tttcctttct aaacaggagt atgtatgacc
     1261 aagtcattgt cgaatgtacc aattccagag accccggtag atgagcttag ctacgaacaa
     1321 gccctcgctg agctggaggg aattgttgcc ggtcttgaaa cgggtgacca tgccctggag
     1381 gcttccctgg cgctctacga gcgcggccag ctgttagtgc gttactgcga gaaattgctg
     1441 gaaaaggcgg agctgaaaat tcaatcgctt tccggagagg atctggtcga ttttggccaa
     1501 caatctgatt gaatatggca gacctgtttt caaattacgc gctcatcgct ggattggtag
     1561 cctgggggat tgcccaggtt gtcaaggtcc cccttgaata tttgcaaacg cgaacctgga
     1621 actgggtgct tcttttgcgc gctggcggga tgcccagctc gcactcagcc ctggtcaccg
     1681 ccatatccta cgccactggc ttgtttatcg gatttgacca accggtcttt gccttatcgg
     1741 ttgcggtggc gatggtggtg gtctacgatg ccaccggaat ccgccgccag gctggcaagc
     1801 atgctgcttt gatcaacgct atgatcaacg acctggctag tggtcaccca ctcaaagagg
     1861 agcagttacg cgaggtgctg ggtcacactc cgttggaggc gattggagga gttgttttgg
     1921 ggttagtgac cgcccaattg gtgtggttca tctgggggtg aaattcttgc gattacgagc
     1981 gatgtatcca ctgatcatag ccaccatgcc cttcacttcc ttcactccca ttacccacat
     2041 cactacagag tagattcccc ctcctatcac cagacctccc agggtagttc tccaggcagg
     2101 cgactcgccg gcccacgcca accaaatcca tatccctgct gacatgacca gcgcgccagc
     2161 tgctgcctgc aggcttcctc ttagaatgaa atcgccctgc attccctcca gtctacggcg
     2221 catatagtag accaatccaa ccgcctctaa agccgtcgcc agcgtgtttg ccagggctag
     2281 ccctccatga ggcaaccagc ctatgcggga gaatagggca gaaaagagca ggctgaagat
     2341 tacgtttagc gccatcgcca acgcgcccac caggacaggg gtgcgcgtat cgtgcagagc
     2401 gtagaaggcg cgcgccagaa cttcaaccag ggagtggccc accagcccgg ctgcatacca
     2461 tagcaatgcc caggctacca gttccgtgga gcgtgaatca aaagcgccgc gctggtagag
     2521 cagggtgata accggctggc gcaacagcat caaacccagg ctggctggta tggagagcag
     2581 cagtaccccc cgcaggctat cggctaatga gcggcgcatc tcgcgcagtt gaccgcgagc
     2641 atactgcgct gccaaagtgg gcatggcagc gatagctact gattgagcga tggctgcctg
     2701 gggcatgagc atcagcgcaa aggcggtgac aatggcggtc acgctgccct ctggctgcag
     2761 agaagccaag cgagtattca cccagaagtt gagctgcacc actgccaccc ccaatagccg
     2821 cggccctagt agccgcgcca cctccctcac tggagcgtat tcaataccca aggttgggaa
     2881 gtattgcccc ttttgccgga gaagtgcggg gatttgcaag cccaggtgca atcctgcgcc
     2941 aatcagcacc ccccaggcca gcccgtagat acccatcttg ggagtgagta ccaacactcc
     3001 gaaaattagg cccagttggt acatagaggg tgttagggca gggatgaaaa aaacctggtg
     3061 agcgttgagt atgcccatga tcaacccgct taccccgaag atcgccgctg aaggcagcat
     3121 catgcgcatc agcgagatag taagagcttc tttggctggc tcactagcga agccgggtgc
     3181 tagcacggag cgcgtgatct gcggggcgaa aagcgcagct aggacgatgg cgccgaccat
     3241 ggtgaataag attagattga aaatagacga ggccagccgc caggcgccct ttttatcctc
     3301 ctgggtaagt atcccagtaa aagttggtat gaaagccgag gctagcgccc cgcccgctac
//
LOCUS       DBNHOH010000339         3511 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_211688, whole genome shotgun sequence.
ACCESSION   DBNHOH010000339 DBNHOH010000000
VERSION     DBNHOH010000339.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_211688"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(541..774)
                     /locus_tag="AC16V7_06085"
     CDS             complement(541..774)
                     /locus_tag="AC16V7_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256723.1"
                     /translation="MEVILTTLASMFKTDERFIMHRYYSTRISMMVGIILIVAWFSYE
                     LLVNNLLRWDLAIIAGAMAITKILAMLYFRATG"
     gene            complement(765..989)
                     /locus_tag="AC16V7_06090"
     CDS             complement(765..989)
                     /locus_tag="AC16V7_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015321249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR6256724.1"
                     /translation="MGTEVRNQIKERRLRHGLSQEELAKAVGVSRQSINSIERGRYIP
                     SLPLALRFARLFDCPLDDLFQLQEEGETWK"
     gene            complement(1060..2031)
                     /locus_tag="AC16V7_06095"
     CDS             complement(1060..2031)
                     /locus_tag="AC16V7_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6256725.1"
                     /translation="MLERTPAIVAHKLTKRFGDFTAVNDVDFEVQRGEIFGFLGPNGS
                     GKTTTIRMLLGLMKPSAGSVEVLNMPVDGDTQKIRPRVGYMSQRFSLYNDLSVLQNLR
                     FYGAAYGLSDAELEVRIGEALVMCGLEGREDSKTKDLSGGWRQRLALSAAILHRPEVL
                     FLDEPTAGVDPVSRRVFWDLLYALVVQGVTVFVTTHYMDEAEHCHRLAFIQHGRIIAC
                     GSPAEIKAHMMQGQVLEIVPSDAIRAVKVLRAAQESGELAIAEVELYGSLVHVVAPRM
                     QEQQRLIAAKLGQSGIQAGQMSIIEPSLEDVFIACMKGKAARDPYKT"
     gene            complement(2036..3160)
                     /locus_tag="AC16V7_06100"
     CDS             complement(2036..3160)
                     /locus_tag="AC16V7_06100"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6256726.1"
                     /translation="MLRRIWILVIKEFIHLRTDWWLPAFMLLGGAMELLLVGWATSRP
                     ITNLPLMVLDQCNSPASRSLLVALENTGTFQLQEQVSDMVTIESALEQGKINAALVIP
                     PDFSEQINSSYGRPTLLAILNGAESTPAMAALRAIDGVSREVDKRILIQRLGLSADEF
                     SDFNLSLRVWFNEELSEALYTTPAELGLMLEFTVLLFAALAFSRERELGTLEQLLVMP
                     FSSLEIIIGKAIPVIIVGFTDFLLMLGMVHFAFGVPVRGSLPLLLVLALGYLLVELSK
                     GMVISVISRTQHQAFLLVMLLGMVDFMFTGYAAPVDGMPKALQYFANLIPAHHWLSIL
                     RGILLKGAGLDVLWQHVLTLFILGLLIGTFSWRFVRRALD"
     gene            complement(3164..>3511)
                     /locus_tag="AC16V7_06105"
     CDS             complement(3164..>3511)
                     /locus_tag="AC16V7_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6256727.1"
                     /translation="LYFGLSAIFLFAVLSMGMIVGVFMRTQAAALALSFLVIFFPGFF
                     LTGIFFPLASMPEEVRMEAMMLPGTHYAVITRGVFLPGVGLEVLWPYAVMLIVLGLAF
                     TGLAALFFRKKLG"
BASE COUNT          933 a          980 c          865 g          733 t
ORIGIN      
        1 aatggatgac gtgcttcgta ttcgggcgaa acgggtaaac cgaaacatct gccaccaggt
       61 agccattccg gataattgaa acgctgtcaa tttcgtagcc ccgctccaag atttccccca
      121 tcatttctgc taacaattcc gaatccatgc cttgttgctc aggcgtagag gtccgaaaac
      181 ccttggttgg ccagtattcg acaaattcct gtgtcgcaac cggtgccacg gcgggtgtaa
      241 cttgagcgca tccagaaagg aacgcgagga tacaaaacag aagaagagat ctgctgattt
      301 ttaggttgaa gatcatcaca cccccggagt tctagctaat ttttgttaat gtaaaccagt
      361 caatataaca ataatactcc cgacactcgc cgggcgttta aatttccttg aacccgatgt
      421 atggaatatg cagatgaaaa gctgggatca cctggctgta caaccggaat gatcaaagag
      481 tgttgatcac aatgctagtc cacttgccga aggaggctat agccaactgc atccatgcaa
      541 ttaaccagtg gctcggaaat agagcatcgc cagaattttt gtgatcgcca tcgcccccgc
      601 aatgatagcc agatcccagc gcagtagatt attgactaaa agctcatagc tgaaccaagc
      661 cacaatcaga atgatcccga ccatcatgga tattcgagta gagtaatacc tgtgcatgat
      721 gaagcgctca tccgttttga acatgctggc cagtgtggta agtatcactt ccatgtctcg
      781 ccttcctcct ggagttggaa caaatcatcc aacggacaat caaataatcg ggcaaatctc
      841 aacgcgagtg gtaagctggg tatgtaccgc cctcgctcga tggagttgat gctctgacga
      901 gacacaccga cagccttcgc gagctcttct tgagagagac cgtgccgcaa gcggcgttct
      961 ttgatttgat tcctgacttc agtgcccatt ctaaattccg agtagatgtc aagtctactt
     1021 gacatcatac acgagaaatt ccgcggtgtc aagttggctt tacgttttgt agggatctcg
     1081 agctgcttta cccttcatgc aggcgatgaa cacgtcttcc agggaaggct cgatgatgga
     1141 catctgtcca gcctggatgc cagactggcc taacttggcg gcgatgaggc gctgctgctc
     1201 ctgcatccgc ggcgcgacta cgtgcacgag tgagccatac aattcgactt cggcaatggc
     1261 tagctcaccc gactcttgag cagcacgcag caccttgacc gctcggatgg cgtcggaagg
     1321 gacaatttca agcacctgac cttgcatcat gtgcgctttg atctccgcag gcgagccgca
     1381 ggcaatgata cgcccatgct ggataaatgc cagcctatgg caatgttctg cttcatccat
     1441 atagtgtgtg gtgacaaaga ccgtcacccc ctgaacaacc agggcataca gcaaatccca
     1501 gaaaacgcga cgcgagaccg ggtccacgcc cgctgtaggc tcgtccaaga aaagcacttc
     1561 aggacggtgc aagatagccg cgctaagagc caggcgctgt cgccaccctc cagataggtc
     1621 tttggttttg gaatcttcgc gcccttccag cccacacatc accagggctt caccaatgcg
     1681 cacttccaat tcagcgtcag acaatccgta tgcagctccg taaaagcgca ggttttgcag
     1741 cacgctcagg tcattataca aactgaaacg ctgcgacatg taccccacac gcgggcgaat
     1801 cttttgagtg tctccatcca caggcatatt gagcacttcc accgacccgg cgcttggctt
     1861 catcagcccc agcaacatgc ggattgtggt agttttgccc gatccattgg gacccaagaa
     1921 gccaaaaatc tccccgcgct gtacttcaaa gtccacatcg ttcacagcgg tgaagtcgcc
     1981 gaagcgtttg gttaatttgt gcgccacgat ggcaggtgtt ctttccaaca tcgatttaat
     2041 ccaatgcccg gcggacgaag cgccaagaga aagtaccaat cagcaatccc aagatgaaca
     2101 gggtcaaaac atgctgccag agcacatcca gcccggcgcc tttgagcagt atgccgcgca
     2161 gaatgctcaa ccaatggtga gcgggaatca ggttagcaaa gtattgcaag gctttgggca
     2221 taccatccac gggagcagca tagccggtga acatgaaatc caccataccc agcagcatca
     2281 ccagtaaaaa ggcttgatgc tgggtgcgtg agataaccga aatgaccatg cccttgctca
     2341 actcaactaa caggtagccc agagccaaaa ccaggagcag tggcaacgat ccgcgcactg
     2401 gcacgccaaa agcgaaatgt accatgccaa gcatgagcag aaaatcggta aagcccacga
     2461 tgatcactgg gatggctttg ccgatgatga tctccagcga ggagaatggc attaccagca
     2521 gttgttcaag tgtccccaat tcgcgttcgc gtgaaaaggc caaggctgca aagagcagca
     2581 ctgtaaactc tagcatcagg cccagctcag ccggagtagt gtataaagct tcgctgagct
     2641 cttcattgaa ccacacccgc aggctaagat tgaaatccga aaattcatcc gcgctcaagc
     2701 ctagtcgctg gatgagtatg cgtttatcta cctctcggct gactccatcg atggcgcgca
     2761 gcgcggccat cgccggcgtg ctttctgcac cgtttaggat cgcaagcagt gtcgggcgcc
     2821 cataagacga gttgatctgc tcgctgaaat ctggtgggat aaccaaagcc gcattgatct
     2881 tgccctgctc cagtgccgat tcaatcgtga ccatgtcgct aacctgctcc tgtagctgaa
     2941 acgtgcccgt attctctaga gctaccaaaa gagatcgact ggcagggcta ttgcactggt
     3001 cgagcaccat caaaggcaga ttggtgatcg gacgcgaggt cgcccagcca accagcaaaa
     3061 gctccatcgc cccacccagc agcatgaagg cgggtagcca ccagtctgtg cgcaagtgga
     3121 tgaactcttt gatcaccagt atccagattc gcctcagcat gagctatccc aatttcttac
     3181 ggaaaaacaa ggctgccagt cccgtaaatg caagacccag cacgatgagc atcacggcat
     3241 acggccacaa cacctccaga cccacgcctg gcaggaagac cccgcgcgtg atcaccgcgt
     3301 agtgcgtgcc cggcagcatc atggcttcca tgcgcacttc ttcaggcatt gaagccaatg
     3361 ggaagaaaat accggtcagg aagaagccag ggaagaatat caccaggaac gaaagtgcaa
     3421 gcgcagcagc ctgagtacgc ataaacacgc ccacaatcat gcccatgctc agcacagcaa
     3481 acaggaagat cgccgagagg ccaaaatata g
//
LOCUS       DBNHOH010000340         4934 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1750139, whole genome shotgun sequence.
ACCESSION   DBNHOH010000340 DBNHOH010000000
VERSION     DBNHOH010000340.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4934)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1750139"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="AC16V7_06110"
     CDS             <1..345
                     /locus_tag="AC16V7_06110"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6256728.1"
                     /translation="EHDWTQRPGLLVASLNEETVGWIGLRLDIAPVTAWATDLGVLRR
                     LRRHGIGSALVLAAQEWGETHDCRRLVLEMQPKNYPAIQMAHKLGLDFCGYNDRYYAN
                     HDIGLFFAKSLR"
     gene            388..2349
                     /locus_tag="AC16V7_06115"
     CDS             388..2349
                     /locus_tag="AC16V7_06115"
                     /inference="COORDINATES: protein motif:HMM:NF028237.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HIR6256729.1"
                     /translation="MVDLFVSTLVTLNQLLTAGVAITAFSLLLYALTFNLRDRVARSF
                     AWILTCVVIVFASDAISSTTNSPADLVFWLKLQWVGIVFLPATYLHFSDGLLATTGRP
                     SRGRRRMVVRLSYGLSLAFLATLPFNLLVGSLVQGEQPAPHLDRTWLTWVFSIYFGIA
                     IAFAMINFARAYRRTLASTSRRRMGYLLVGALAPALGSFPYLLFGSGLASRHTLLFWI
                     IVTLSNLAISALLVLMAYAVAFFGVNWPDRVVKRRLFKWLMRGPVTASTVLAATTLAR
                     RAGESFGQSYTTAVPIIMVALILLLEYLITLAAPVWERWLFHGGDRENIEMLQNLEER
                     LLTLGDLRQFLESVLAAVCDRLQASNAFVAALGQNGLEMVVTIGSNHILKQEDLSANL
                     LEQVNQNGLHTNLFSWGGYWLVPLYAHQDDQDELLGLMGTKQQPGYEIDEEQQEALSI
                     LANRAALALEDRRAQEQLFTSVEALTPAVELIQNLRAATRYGGGGALGELLTQPELSK
                     EKGAFSSWVKDALTHYWGGPKLSQSPLIKLRVVQQALEEHEGNPTNALRAILRQAIDR
                     VRPEGDRRFTAEWILYNILELKFMEGRKVREVALRLAMSEADLYRKQRVAIEEVAKAI
                     IDMETIAVDEDNVAMGVATTEANHYTRWA"
     gene            2349..3335
                     /locus_tag="AC16V7_06120"
     CDS             2349..3335
                     /locus_tag="AC16V7_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012784.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIR6256730.1"
                     /translation="MVNKQPYKFTNELSEGPPPLDESWWDAVMAEEGAHKPTSPRSGS
                     TRPTTNDKASAAHTAEHPTPVDWDMAIELYESDKIVTLQVSGYNRGGLLVNGDGLQGF
                     VPVSHLVELPAEKSAAEQELALSEYVGRDLELKIIECDPSRGRVVYSERAALAEPGSR
                     NTLLHQLQSGQCVQGTVTNITDFGVFVDLGGVEGLVHVSEISWGRVRHPADVVQYGEP
                     LQVQVINVDPERSRVALSIKRLNQNPWETAETRYFPGQVTEAVITSLVPFGAFARLEE
                     GLDGLIHISEINSGNPPANLNNLLQEGQRVTVRVLHVDSANQRLGLSMVVQE"
     gene            3395..3985
                     /locus_tag="AC16V7_06125"
     CDS             3395..3985
                     /locus_tag="AC16V7_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR6256731.1"
                     /translation="MKSWWGILWPISLGLMLGLLASGLILLVSSQPRGEAVRLSPPPT
                     APPLVVHVSGAVSQPGVFNLMQGSRVQDAIQAAGGITPDADAQALNLAAPLEDGVLVY
                     VPLKSQEGPATMSPGISVPSPAGNPGPTAGGLININTASQSELESLPGIGPVLAQRII
                     EYRQSHGAFSSTEAIIDVSGIGPGIFEKIKDLITVN"
     gene            complement(4011..>4934)
                     /locus_tag="AC16V7_06130"
     CDS             complement(4011..>4934)
                     /locus_tag="AC16V7_06130"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6256732.1"
                     /translation="YQIAEGVYAAIGLIGGAAYSNAGIIDLGDRALIFDTFETPTAAD
                     DLRAAAEQLVGNQFSYIIISHAHTDHWMGNQSFPESVAIITTQVIRDEMYEYGAEMRE
                     LQQNPSEFEEEIREYEERLQAETDEGQRASLQATISRYRYSLEMLPDLKLRFPNQTFD
                     GKLVFHGSQRAVELVASGKGHTASDCFLVLPAEKIAFIGDLGFFQCQPYMVYCDPDAW
                     VAQLEAMEAWDIETFVPGHGPVGSKVDLALQKEYIRTLDQLVARVIGEGGTVEDALRQ
                     PLPAPFDAWLPAGRVRFEANMRSSYKRLFGG"
BASE COUNT         1114 a         1338 c         1367 g         1115 t
ORIGIN      
        1 gaacacgatt ggacacaacg acctggatta ttggtggcct cgctaaatga agagacggta
       61 ggctggatcg gtttgaggct ggacatcgcc ccggtcaccg cttgggctac cgacctggga
      121 gtgttgcgcc ggctgcgccg ccatgggatc ggcagcgctc tggtgctggc agctcaggaa
      181 tggggcgaga cgcatgactg ccggcggctc gttttggaga tgcagcccaa aaactacccg
      241 gctatccaga tggcgcataa attaggtcta gatttttgcg gctacaacga tcgctactac
      301 gcgaatcatg atattggttt attttttgca aagtctttaa gatagtatgc tatgccatct
      361 tgaaccgtag taaactatta acaaggtatg gtcgatctgt ttgtctctac cctggtaact
      421 cttaaccaac tgctcaccgc gggagttgcc atcacggcat tctcgctact gctatacgcg
      481 ctgactttta atttgcgcga tcgagtggcg cgctcattcg cctggatcct gacatgtgta
      541 gtcatcgtct tcgccagcga tgccatatca tccaccacca attcgcctgc agacctggta
      601 ttttggctca agctccaatg ggtaggtatc gttttcctgc cagccactta cctgcatttt
      661 tccgatggac tcttagccac caccggacgt ccatcgcgtg gaagacgccg catggttgtg
      721 cgcctgtcct atggcctttc actggctttc ttagccacat tgcccttcaa cctgcttgtc
      781 ggttcgctgg tacagggcga gcaacctgcg cctcacctgg accgcacctg gctcacctgg
      841 gtcttcagca tctacttcgg catcgcgatc gcctttgcga tgatcaactt tgcccgcgcc
      901 taccgacgaa ccctggcgag caccagccga aggcgtatgg gctacctgct ggtaggggcg
      961 cttgcgcccg ctttgggctc tttcccgtac ctgttattcg gttctggcct ggctagcaga
     1021 catacactgc ttttctggat aattgtcaca ttaagcaacc tggctatctc tgcgttgctg
     1081 gtcttaatgg catacgcggt ggcgttcttt ggggtaaact ggcccgaccg ggtggtcaag
     1141 cgacgccttt tcaagtggtt aatgcggggt ccggtaaccg cctccaccgt gctggctgcc
     1201 accacgctcg cccggcgggc tggggagtcc tttgggcaaa gctacacaac cgcggtgccc
     1261 atcattatgg tagccttgat tctcctcctg gagtacctga tcactctcgc agcacctgta
     1321 tgggagcgct ggctatttca tggaggcgat cgcgaaaaca tcgagatgct gcaaaaccta
     1381 gaagaacgac tgctgacttt aggggatctg aggcagttcc tggaatcggt gctggctgcg
     1441 gtgtgcgatc gcttgcaggc ctccaatgct ttcgtggcgg cacttggaca gaacgggtta
     1501 gagatggtag ttaccatcgg cagcaatcac attcttaaac aggaggatct atcggcaaat
     1561 ttactggaac aagtcaatca aaacggtctg cacacaaatc tgttctcatg ggggggatac
     1621 tggctggtac ccctgtatgc ccatcaagac gatcaggatg aactattggg cttgatgggg
     1681 accaaacagc agcctggata tgaaattgat gaagagcagc aggaagcgct ctcgatactg
     1741 gcaaaccggg ctgcactggc attagaagac cggcgggcgc aggagcaatt atttacctct
     1801 gtcgaagcat tgacacctgc ggtagaattg atccaaaatc tacgcgcagc cacgcgctac
     1861 ggcggtgggg gggcactcgg agagctactc actcagcccg agctctccaa agagaagggc
     1921 gccttctcta gttgggttaa agatgcactt actcactatt ggggaggtcc aaagctatct
     1981 cagagtccat tgatcaaact gcgcgtggtg caacaggcgc tggaggaaca cgaggggaac
     2041 cccaccaacg ctttgcgagc tatcctgcgc caagctattg accgagtcag accggaggga
     2101 gatcgacgct ttacagcaga atggattttg tataatatat tagaattgaa attcatggaa
     2161 ggtcgcaaag tgcgcgaggt agctttacgc ctcgccatgt ctgaagctga cctgtatcgt
     2221 aagcaacggg tagcaatcga ggaggtcgcc aaagctatta tagatatgga aacaatcgcc
     2281 gtcgatgaag ataatgtggc gatgggggtc gctacgacag aagctaatca ttacacaagg
     2341 tgggcttaat ggttaacaaa caaccttaca aatttactaa cgaactgagc gagggtccac
     2401 ctccgctaga tgaatcttgg tgggatgcag tcatggcgga agagggtgct cataaaccaa
     2461 catcacccag gtccgggtca acccggccca cgacaaatga caaagcaagt gctgcgcaca
     2521 cggctgaaca tccaactcca gtcgactggg acatggctat agagctgtat gaatcagata
     2581 agatcgtcac cttgcaagta agcggatata accggggtgg attgctggtc aatggagatg
     2641 gattgcaagg ctttgtgccc gtttcgcacc tggtcgagct gcctgcagaa aaaagcgccg
     2701 cagagcagga actggcccta agcgagtatg tcggcaggga tttagaactt aagattatcg
     2761 agtgtgaccc ttccaggggg agggtggtct attctgaacg cgccgcactg gctgagccag
     2821 gcagccgcaa cacgctgcta caccaattgc aatccggcca atgcgtgcag ggcacagtca
     2881 ccaatatcac tgattttgga gtctttgtag atctgggggg cgtggagggg ctggttcacg
     2941 tatccgagat ttcttggggg cgcgtgcggc acccagcgga tgttgtccaa tatggggagc
     3001 cactgcaggt tcaggtgatc aatgtagacc cagagcgctc tcgagtggct ctcagtatca
     3061 agaggttaaa ccagaacccg tgggaaacag ccgaaacgcg ctatttcccc gggcaggtta
     3121 ccgaggcggt tatcaccagc ctggtaccct ttggcgcctt tgcccgcctt gaagaaggtc
     3181 tggacgggct gattcacatc tccgaaatta actcaggcaa ccctccggca aacctgaata
     3241 acttactgca ggaggggcag cgcgttacgg ttcgcgtgct gcatgtggac tccgccaacc
     3301 agcgcctggg gctgagtatg gtcgtccaag agtaatatct ctcctccccc aatcacgggg
     3361 gagtgacttg ggtaggggaa ggtaagtaag tgccatgaag tcctggtggg gtattttgtg
     3421 gcctatcagc ctggggctaa tgcttggctt gcttgcttcg ggtttaatct tgctggtcag
     3481 cagccagcca cgcggcgagg cggtccgcct ctctccacct cccacagcgc cacctctcgt
     3541 cgtccacgtg agcggcgctg tttctcaacc gggcgtcttc aatctaatgc aaggcagccg
     3601 cgtccaggat gcgatacagg ctgcgggggg catcacccca gatgcagatg cgcaagccct
     3661 caacctagcc gctcccctgg aggatggggt gctggtctat gtccccctga aaagccaaga
     3721 ggggcctgcc acaatgtcac cgggcatatc cgttccctcc cccgcgggaa accccggccc
     3781 gacagcggga ggcctgatca atatcaacac ggcctctcaa agcgagctgg agagcctgcc
     3841 gggcatcggt ccagtgctgg cacagcgcat catcgaatac cggcagagtc acggagcttt
     3901 ttcaagcacc gaggcgatta tagatgtctc tggcattgga ccagggatat ttgagaaaat
     3961 caaagacctc atcacggtga actaaacggt tgcgctggct ggattgctgc tcatccaccg
     4021 aaaagtcgtt tgtaggatga gcgcatatta gcttcaaagc gcacccttcc ggctggcaac
     4081 cacgcatcaa acggcgcggg aagcggctgg cgcagcgcat cttccaccgt tccaccttct
     4141 ccaatcacac gggcaaccag ctgatccagc gtgcggatgt attctttctg caatgccagg
     4201 tccactttgc tccccaccgg accatgcccg ggcacgaagg tttcaatgtc ccaagcttcc
     4261 atcgcttcta gctgggccac ccaggcatcg ggatcgcagt aaaccatgta gggctggcat
     4321 tgaaagaatc ccaaatcacc tataaaggca atcttctcgg ctggcaatac gagaaagcaa
     4381 tcgctggcag tgtggccctt gcctgaggcg actaattcca ctgcccgctg cgagccatga
     4441 aataccagct tcccgtcaaa ggtttggttt ggaaatcgaa gtttgaggtc aggcaacatc
     4501 tcaagtgaat agcgatagcg tgaaatcgta gcttggagag atgccctctg gccctcgtct
     4561 gtctccgcct gaaggcgttc ttcatactcc cgaatctcct cttcaaactc tgatggattt
     4621 tgctgaagct ctctcatttc agcgccatat tcgtacatct cgtcgcggat cacctgggtg
     4681 gtgatgatgg caaccgactc agggaaagat tggttgccca tccaatgatc cgtgtgggcg
     4741 tgactgataa tgatgtagct gaactgattc ccgaccaatt gctcagcggc agctcgcaaa
     4801 tcatctgctg cagtgggcgt ctcgaaggtg tcaaagatca atgcgcgatc gcccagatcg
     4861 atgatgccag cattgctgta tgccgcacca ccaataaggc cgatcgcggc gtagaccccc
     4921 tcggctattt gata
//
LOCUS       DBNHOH010000341         3555 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_275587, whole genome shotgun sequence.
ACCESSION   DBNHOH010000341 DBNHOH010000000
VERSION     DBNHOH010000341.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_275587"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="AC16V7_06135"
     CDS             <1..312
                     /locus_tag="AC16V7_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SanA/YdcF family protein"
                     /protein_id="HIR6256733.1"
                     /translation="IVLDYAGQRTYDTCYRAREIFGIKEATLVTQGFHLPRALYTCNQ
                     LGVEAIGVPADLRDYRRGARIFWHGREVPATLVALWEIHITRPKPILGEPEPIFPLEA
                     Q"
     gene            312..866
                     /locus_tag="AC16V7_06140"
     CDS             312..866
                     /locus_tag="AC16V7_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HIR6256734.1"
                     /translation="MDRSEALAFVREHVNNENLVRHMLAVEAAMRFYAEMFGEDVELW
                     GLIGLLHDFDWEIHPTLEEHPQAGAPILRARGVDEVIVRAILSHADHTGVPRNTRMEK
                     ALLACDEITGLITAVALVRPSRSLHDLEAGSVKKKWKDRAFAAGANRGEISHAVEDFG
                     IDLWEHVGNVIQAMRRIAPQLALD"
     gene            981..2927
                     /locus_tag="AC16V7_06145"
     CDS             981..2927
                     /locus_tag="AC16V7_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR6256735.1"
                     /translation="MIKVEEENRGSVVLRRAAEYTPPSSWRTWLIGRPLQTADAPHQT
                     IGKAIGLAVFASDALSSTAYATQEILIILAVAGTVAFGYVFPISIAIVILMAIVTISY
                     QQTIHAYPGGGGAYIVSRDNLGELPAQIAGAALLTDYILTVAVSVSSGVAQIVSAYPE
                     IFAYRVHISVILVFFIMLINMRGVKESGAAFALPTYFFVVMMYITVGVGLFRALTGSL
                     GPVIDPPELEAIHALQGVTAFLILHAFSSGTAALTGIEAISNGITAFKEPRSRNAGIT
                     LIWMALILGSLFLGISYLAREIGTIPSEIETVISQLARTIFHGQAGLYLAVIAATTVI
                     LVMAANTAFADFPRLSALLAADGFLPRQLTYRGSRLVFSRGIALLALVASLLIIVFNA
                     SVTRLIPLYAIGVFSSFTLSQAGMARRWWKIGHLEPGQELKERGSMLRYESGWQLKMV
                     INGFGALCTLVVMFVFAITKFRDGAWIVLILTPSLVAIFSSIHQHYRNLAKRLSLDNY
                     GLPPRIPRHRVILPISGVHRGSLAALQYARTLSDDITAVHVCTDPEEASKTQQKWEKW
                     GDGVRLVLLDSPYRLLAEPLLEYIESIANQRQPNAIITVIVPQFVPKRWINNLLHTQT
                     ALWLRLALLFKPGIVITDVPYQVE"
     gene            2943..>3555
                     /locus_tag="AC16V7_06150"
     CDS             2943..>3555
                     /locus_tag="AC16V7_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR6256736.1"
                     /translation="MNIIIVGCGRVGAELAYRLYQKGHNISVIDHVASAFNNLPSDFR
                     GRTIEGEVMNMDVLSRAGIDSADGLAAVTNSDPLNAVVAHLARSAYGVPNIVVRNYDP
                     SLRVLHETFDLQVVSSSSWGAQRLEEMLASVNLRTVFSAGNGEVEIYEFSVPAAWDGA
                     TLGELLPTKNGIIISVTRAGKAILPTPDYQIKTNDVILVSATFD"
BASE COUNT          787 a          965 c          928 g          875 t
ORIGIN      
        1 atcgtcttgg attacgccgg ccaacgcact tacgacacct gttatcgcgc tagggaaatt
       61 ttcggcataa aagaagctac tcttgtcacg caaggctttc acctgccgcg cgccttgtac
      121 acatgcaacc agctcggtgt cgaagccatc ggagtgcctg cagatctgcg agactaccgt
      181 cgtggcgcga ggattttctg gcatggccgc gaggtccctg ccactctggt agccttatgg
      241 gagatacaca tcacccgacc aaagcctatt ttaggcgagc ccgaacccat ctttcccctg
      301 gaggcgcaat aatggatcga tccgaagccc tggcttttgt ccgagagcat gtcaataatg
      361 aaaatcttgt gcgccatatg ctggctgtcg aagcggctat gcgtttctac gcagagatgt
      421 ttggtgaaga cgtggagtta tgggggctga ttggtctgct gcacgatttc gactgggaga
      481 tccatccaac cctggaagag catcctcagg caggggcgcc aatcctgcgg gcgcgcggcg
      541 tagatgaagt catcgtgcgc gcgatcctca gccacgccga tcacacaggt gtgccacgca
      601 atacccgtat ggagaaagcc ctgctggcct gcgacgagat caccgggttg atcacagcgg
      661 tggcacttgt gcgcccatct cgctcgttac atgacctcga ggcgggatcc gtgaaaaaga
      721 agtggaaaga ccgcgccttt gcagccggtg ccaaccgggg agaaatctca cacgcggtag
      781 aagatttcgg catcgatttg tgggagcacg tgggaaatgt aattcaagct atgcgccgca
      841 tcgccccaca gctagccttg gattgaaatt tccttattga cgcttcgttc gatattgaat
      901 agaatcaaag agcttaccag cccgccgggt gctatcttcg gatcgcggtg ggctttcgtt
      961 tgctattaag gaaaatgcac atgattaagg ttgaagaaga aaatcgcggc tcggttgttt
     1021 taaggcgcgc cgcagaatac acaccgcctt cgtcctggcg cacgtggtta attggtcgcc
     1081 cgctgcaaac tgctgacgcg ccgcatcaaa cgattggcaa agccatcggg ctggcagtat
     1141 tcgcctccga tgctctctcg tctaccgcat acgccaccca ggaaatcttg attatcctgg
     1201 ctgtagcggg taccgttgcc tttgggtacg tattccccat ctccatcgcg attgtgatct
     1261 tgatggcgat cgtaaccata tcgtatcagc agacaatcca cgcctatcca gggggaggtg
     1321 gcgcctatat tgtctcgcga gacaatctgg gtgaattacc tgcccagata gccggtgccg
     1381 cgctgctcac cgattacatc ttgacagttg cagtttctgt ctcatctggg gtagcgcaga
     1441 ttgtctccgc ttacccagag atattcgctt accgagtgca catttccgtg atcctagtat
     1501 tttttattat gttgatcaac atgcgcggcg ttaaagaatc aggcgcggcg tttgcactcc
     1561 ctacctactt ttttgttgtc atgatgtaca taactgtcgg agttgggcta tttcgagcct
     1621 tgactggctc cttgggacct gtaatcgatc cacccgaatt agaagctatt cacgccctgc
     1681 aaggggttac ggcgttcctg atactacacg cattttccag cggcacagct gctttgactg
     1741 gaatagaagc catatccaat ggcatcacag catttaaaga accgcgcagc cgcaacgctg
     1801 gcatcacatt gatctggatg gctctcatcc tgggttccct gttcctgggg atttcgtacc
     1861 tggcgcgcga aattggcacc atcccatccg agatcgaaac cgtcatctcg cagctcgccc
     1921 gcacaatatt tcacgggcaa gcaggtttgt acctggctgt aattgcggcc accaccgtga
     1981 tattggtcat ggcagccaat accgccttcg cagatttccc ccgcctcagc gccttgctcg
     2041 ctgcggatgg ctttttacca cgccagttaa cctaccgtgg cagcagactg gtgttctcac
     2101 gaggcattgc gcttctagcc ttggtggcct cgcttttgat tattgtattc aacgccagcg
     2161 tgacccgttt gatccctttg tacgctatcg gcgtgttcag ttcattcacg ctatcgcaag
     2221 ctggcatggc gcgcaggtgg tggaaaattg gacacctgga accaggtcag gaactcaaag
     2281 aacgcggttc aatgcttcgt tatgaatccg gctggcaact caagatggtt atcaatggat
     2341 tcggagcgtt gtgcaccctg gtggtcatgt ttgtatttgc aataaccaag ttccgcgatg
     2401 gggcatggat cgtcctgatc ctaaccccaa gcctggtcgc cattttctcc tccattcacc
     2461 aacattacag gaatttggcg aaacgcctct cgttggataa ttatggtctg cccccacgta
     2521 tcccaaggca ccgcgtcatc ctgcccatca gtggtgtgca ccggggttca ttggcagcct
     2581 tgcaatacgc gcgtactctg agcgatgata tcaccgccgt gcacgtttgc accgacccgg
     2641 aagaagccag caaaacccag cagaagtggg agaaatgggg ggatggcgtg cgcctggtgt
     2701 tgctcgattc accatatcgc ttgttagctg aacccttgct ggagtatatt gaatcgattg
     2761 ccaatcaacg ccaaccaaac gcgatcatca ctgtcatcgt gccacaattt gtccccaaac
     2821 gctggataaa caatctgctg cacacccaaa ccgcactctg gttgcgattg gcgttattat
     2881 ttaaaccagg gatcgtcatc accgacgtgc cttatcaagt tgagtaaagg agcctctctc
     2941 aaatgaatat catcatcgtc ggttgtggtc gggtaggtgc agaattagcc taccggctct
     3001 accagaaagg gcataacatc tccgtgatcg atcatgtggc atctgcgttc aataaccttc
     3061 cctcagattt tcgtgggcgg acgatagaag gcgaagtgat gaatatggat gtgctcagcc
     3121 gtgcggggat tgatagtgcc gatggtctgg cggcggttac caattccgat ccattgaacg
     3181 ccgtcgtggc tcatctggcg cgaagtgcat acggcgtccc gaacatcgtt gtgcgaaatt
     3241 acgacccaag cctgcgtgtc cttcacgaga cctttgactt gcaagtagtc agttcctcca
     3301 gttggggagc ccaacgcctg gaagagatgc ttgcttccgt aaatttgcgc accgtatttt
     3361 ctgcgggtaa tggtgaggta gagatctacg agttcagcgt accagctgct tgggatggcg
     3421 caaccttggg tgaattgctc cctacaaaga acggcatcat catctccgtg acacgtgccg
     3481 ggaaagccat cttgccaacc ccagattacc agatcaaaac aaatgatgtc atcctggtaa
     3541 gtgctacgtt cgatg
//
LOCUS       DBNHOH010000342         1567 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_147192, whole genome shotgun sequence.
ACCESSION   DBNHOH010000342 DBNHOH010000000
VERSION     DBNHOH010000342.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_147192"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..1081
                     /locus_tag="AC16V7_06155"
     CDS             29..1081
                     /locus_tag="AC16V7_06155"
                     /inference="COORDINATES: protein motif:HMM:NF013324.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR6256737.1"
                     /translation="MARVFDVIEYPNEMREELVHRFPETEAGTFRIGSQVIVRESQAA
                     VFFRDGSALDVFEPGRHTIVTYNIPLLIDKIGKLFNDRTPFPAEVYFVSMREFVDRKW
                     GTPQPIIVRNPNMGLGVALLQGFGNYSFQIKDPQQFVTQVVGAQGIYRTSDIEGRMRA
                     ILLSKLQDLLGETAAKHSVPELIGLTEEIGAGVRAKAQDDFAAVGLTLKTFYVENLKP
                     SSKSAEELRAMGMLDMQTYTQLQAADALRDAAQNPSGGAGLTAGIGAGMGVGNVISQS
                     LSGMTGGGKAGEQAQPSGGPSAPPPVMTPGEAATFLRVSEEDVIAAITAGDLKAKKIG
                     NAYRISKEALDEYLKG"
     gene            complement(1143..>1567)
                     /locus_tag="AC16V7_06160"
     CDS             complement(1143..>1567)
                     /locus_tag="AC16V7_06160"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6256738.1"
                     /translation="VVDEQNKKAVKAGVELRLEVASNIPEINADPKSLERVITVLLDN
                     AIKFSPDGGVTTIEVGFDDYKVWVRVSDSGVGIPEEAMPRLFRRFFRLDEVGGHLFSG
                     AGLGLSIARQVVEQHGGEIHVESQLGKGSTFTINLNRE"
BASE COUNT          407 a          421 c          371 g          368 t
ORIGIN      
        1 attagactaa aaatcaggag attataaaat ggcaagagta tttgatgtca ttgaataccc
       61 caacgagatg cgcgaggagc tcgtacatcg ctttcccgaa acagaggcag gcacttttcg
      121 tattggctca caagtaattg tacgcgaatc ccaggcagcg gtctttttcc gtgatggatc
      181 tgcgctcgac gtctttgaac caggacgtca caccattgtg acctacaata tccccctctt
      241 aatcgacaaa attggcaagt tatttaacga tcggacacct ttcccagcag aagtctattt
      301 cgtctccatg cgtgaatttg tcgaccgaaa atggggtaca cctcaaccga tcattgtgcg
      361 caaccccaac atgggtttag gtgtggccct tctacaaggg ttcggcaatt attccttcca
      421 gatcaaggat ccgcaacagt ttgtcacgca ggtagtcgga gcacagggaa tttaccgcac
      481 atcagacatt gaagggcgca tgcgcgcaat cctgctctcc aagctgcaag atttgctggg
      541 ggagacggct gccaaacaca gcgtacccga actgatcggg ctgaccgaag aaattggcgc
      601 cggcgtgcgc gccaaagctc aggatgattt tgcagcagtc ggtttgacac taaaaacctt
      661 ctacgttgag aatctgaaac cgtctagcaa gtctgccgaa gagctgcgtg ccatgggtat
      721 gttggatatg caaacttata cgcaattgca agccgcggat gcactccgcg atgcagccca
      781 aaatcccagc ggcggcgcag gactcactgc cggcatcggc gctggaatgg gcgttggaaa
      841 tgtaatcagc cagagtctct ccggaatgac cggaggcggc aaggcaggcg agcaggctca
      901 accctctggt gggccatctg cgccaccccc tgtaatgaca cccggcgaag ctgccacttt
      961 tctgcgcgtt tccgaggaag atgtaattgc tgccattact gctggcgact taaaggcaaa
     1021 aaagattggt aatgcttatc gcatcagcaa agaggcctta gacgaatatc tcaagggata
     1081 aaagtaatta atcagaggct gataagtaga agcgagcggg ctatttatgc ccgctcgctt
     1141 tgttattccc tattcagatt tatcgtaaaa gtgctccctt ttcccagctg actctccaca
     1201 tggatctccc ctccgtgttg ctcaaccacc tggcgagcaa tggaaagccc cagtcccgca
     1261 cctgagaaaa ggtgacctcc cacctcatcc aatcgaaaaa aacgtcggaa taatcgcggc
     1321 atggcttcct ctgggatacc aacaccagaa tcgctcaccc gtacccagac tttatagtca
     1381 tcgaaaccca cttctatggt ggttacccca ccatctgggc taaattttat agcgttatca
     1441 agcaacacag tgatgacacg ctccaggctt ttgggatcgg cgttgatttc tggtatatta
     1501 gaggcaacct ccagcctcaa ttcaacccca gcttttacag ctttcttatt ttgttcatcc
     1561 acaaccc
//
LOCUS       DBNHOH010000343         1944 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_981531, whole genome shotgun sequence.
ACCESSION   DBNHOH010000343 DBNHOH010000000
VERSION     DBNHOH010000343.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_981531"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..318
                     /locus_tag="AC16V7_06165"
     CDS             139..318
                     /locus_tag="AC16V7_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256739.1"
                     /translation="MGFRKLRRILGILILLLSLALLIWGLWPYTELTRSTIIQPGEMQ
                     LPTPESFMWMAQYVI"
     gene            335..1066
                     /locus_tag="AC16V7_06170"
     CDS             335..1066
                     /locus_tag="AC16V7_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256740.1"
                     /translation="MQRIRSHLSLTSLVILMLGMPLSACATTGAPPAMAPEASLPTLS
                     PTDVASAPLTQEPLPTQAPAILEARRLNLEWPPTIRVGDSDVIRLTLEVDDQGNLTPT
                     AQIEGHETRGETVLIPDLYDTHNVLAEARLDMSGMQVTPEGEVSEPLAPGQSVSFFWS
                     VRPVDVGTYRGTIWMHLRFIPLDGSAESRKPLTAQLVEVRAVNFLGLGGLAARLLGVL
                     GTLVGSVLGLDNIIPWVIKRFRRKK"
     gene            1114..1710
                     /locus_tag="AC16V7_06175"
     CDS             1114..1710
                     /locus_tag="AC16V7_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6256741.1"
                     /translation="MTNCYLVADPDTGEAAVIDPADNGRTIVGEAERRGWRIGNIWLT
                     HAHFDHLAGASGVADHTNPPPPVALHPEDYPLWRMQGGAMLFGMRIDPGPEPTVDLKH
                     GMILRLGGNQFEVRHAPGHTRGHVILYCAEEKVVFCGDVIFQGSIGRTDLPGGDFNTL
                     IQSIRTQILTLPDDTRLLSGHGPETTVGQERRINPFLL"
BASE COUNT          408 a          482 c          547 g          507 t
ORIGIN      
        1 gttggctgag tttcatgtga cattccttcg tttggttaat aattaccaat tggtggcatg
       61 tacagtgtag catgcttcga tgtattttag aatactttag cggcctaatg gatggtttgc
      121 tgtggtataa tagcagccat gggcttccga aaattaaggc ggattctcgg tattttgatc
      181 ctgctgcttt ccctggcact tctgatctgg ggattatggc cttacactga gctgacccgc
      241 tctaccatca tccagccagg ggaaatgcaa ctgcccaccc cggagagttt catgtggatg
      301 gctcagtatg tcatctagct ggatattgat agccatgcag aggatcaggt cccatctgag
      361 cctaaccagt ttggttatcc tcatgcttgg gatgcccctg agcgcatgtg ccacaactgg
      421 tgctccccca gcgatggccc ctgaagcgtc actgccgact ctaagcccaa cagacgtggc
      481 ttctgctcca ctgacccaag agccgttgcc tactcaagct ccagccattt tggaggcgcg
      541 tcgcctgaac ttggaatggc ctcctacgat ccgggtaggt gactcggatg ttattcgcct
      601 gactctcgag gtggatgatc agggcaacct gactcccacg gcgcaaattg aaggtcacga
      661 aacgcgcgga gagacggtct taatcccaga cctctatgac acacacaatg tactggcgga
      721 ggcgcgcttg gatatgtcag gtatgcaggt gacacctgaa ggggaggtga gtgagccact
      781 agccccaggc cagtcggttt catttttctg gagtgtgcga ccggttgatg tgggtactta
      841 ccgtggtacg atctggatgc atttgcgctt tattcccctg gatggaagcg ctgagagtcg
      901 taaaccgctt actgcccagc ttgttgaagt acgagcagta aacttcctag gcttgggtgg
      961 actggcagcc aggttgctgg gcgtattagg gaccctggtg ggatcagtgc tcgggctcga
     1021 taatatcatc ccatgggtga tcaagagatt tcgtagaaag aagtaaacca gatcgctatg
     1081 ctttcgatag tctcatttac tctgggctct gtaatgacca actgctatct ggtagctgat
     1141 cctgataccg gtgaagcagc ggtgattgat ccggctgata atggtcgtac cattgtcggt
     1201 gaggcagaaa ggcgcggttg gcgcattgga aatatctggc ttactcatgc acacttcgac
     1261 catcttgcag gagcgagtgg ggttgcagat cacactaacc cgccgccacc ggttgccctg
     1321 caccccgaag attatccact atggcgcatg caaggagggg ccatgctgtt tggcatgcgc
     1381 atcgacccgg gccccgagcc gacggtggat ttgaagcatg gcatgatctt gcgcttgggt
     1441 ggaaaccagt ttgaagtccg ccatgctcct ggtcatacgc gcgggcatgt catattgtat
     1501 tgtgctgagg aaaaggtggt tttttgcggt gatgtgatat tccagggcag cattggccgc
     1561 actgatttgc cagggggtga ttttaatacg cttatccaaa gcatccggac ccagatactc
     1621 accctgccag acgacacgcg cctgctttca gggcatggac ccgaaacaac cgttgggcaa
     1681 gagcggcgca tcaatccctt cttgttgtga tgatatctct tgcgagagat aagtaggtcg
     1741 ggtatggcac ccgacctact ctggtttgcg ttccaagccg tcctaacgtt ttttggacag
     1801 cttattattc ttttactgga tgatctgggc agcggtctct actttgcgta atcgatcgtg
     1861 cgggtctctc ggatgaccgt cactttgatt tgcccgggat actgcatgga atcttcaatg
     1921 ttgcgggcga tgtcgcgcgc tagg
//
LOCUS       DBNHOH010000344         1385 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_468861, whole genome shotgun sequence.
ACCESSION   DBNHOH010000344 DBNHOH010000000
VERSION     DBNHOH010000344.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_468861"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="AC16V7_06180"
     CDS             complement(<1..524)
                     /locus_tag="AC16V7_06180"
                     /inference="COORDINATES: protein motif:HMM:NF023222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6256742.1"
                     /translation="MGARTGAQIIEKLREDSPELWHRGERVRDVTTHPALKNGVHSLA
                     ELYDLQWEHPEVMFFDSPSSGDKVGRSYMIPRTQDELKSVSRMMKVWADHYHGMMGRA
                     PDYLNRAISAYAGGADFIAQLDARFADNIRRYHEHIRENDLCLTHTLITPQANRSTGT
                     AEQADPFLAARVKE"
     gene            complement(524..>1385)
                     /locus_tag="AC16V7_06185"
     CDS             complement(524..>1385)
                     /locus_tag="AC16V7_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013144280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR6256743.1"
                     /translation="GETAGASLVAHPGVQLISFTGETSTGKEIMRNGAATLKRYSMEL
                     GGKSPTIIFDDADLERALDGAIWQVYSLNGERCTAGSRLLLQKSVYDDFLALLEARVR
                     NIRVGDPTDPRTEVGPLIHSDHWDRVHGYMDVARQGGASILVGGGRPATLQQGNYFEA
                     TLIAGVSNEMRVAQEEIFGPVLVVIPFDDDADALRMAKDIPYGLAAYVWTNDLKRGHQ
                     IAEAIESGLVWVNSQNVRDLRTPFGGCKASGIGREGGHYSFDFYTEISTIHVALGEHR
                     IPVLGKGGSS"
BASE COUNT          299 a          409 c          361 g          316 t
ORIGIN      
        1 tcttctttca ctcgagcagc caagaatggg tctgcctgtt cggctgttcc cgttgagcga
       61 ttcgcctggg gggtgatcaa ggtatgggtt agacacaggt cgttttcccg gatgtgctcg
      121 tgatagcggc ggatgttgtc cgcaaagcgc gcatctagct gtgcgataaa gtcagctcct
      181 ccggcgtaag ctgaaattgc ccggttgaga taatctgggg cacgtcccat catgccgtgg
      241 tagtggtcag cccatacttt catcatgcga ctcacgctct tcagctcgtc ctgagtgcgt
      301 gggatcatat aggagcgccc aaccttgtcg cccgaggaag gagagtcaaa aaacatgacc
      361 tcgggatgct cccattgcag gtcgtaaagc tcagccagac tgtgcacccc atttttcagg
      421 gcaggatgtg tggtaacatc tcgcaccctt tcgccgcgat gccacagttc tggagaatcc
      481 tcgcgcagtt tttcaataat ttgtgcacca gtacgcgcgc ccattacgaa ctacctcctt
      541 ttcctaatac gggtatgcga tgttctccca gagcaacatg gatggttgaa atctcggtgt
      601 agaagtcgaa gctgtaatgc ccgccttccc ggccgattcc actggctttg cagccgccaa
      661 atggcgtgcg cagatcgcgc acattctgag agttcaccca cactaacccc gactcaatag
      721 cttcagcgat ttggtgtcct cgcttcaggt cgtttgtcca cacataagct gccagcccat
      781 aagggatatc cttagccatc cgcagtgcat cggcgtcatc atcaaagggg ataaccacca
      841 gcaccgggcc aaaaatttcc tcctgggcga cccgcatctc gttgctcacc ccggcgatca
      901 gggtagcttc gaaatagttg ccctgctgaa gagtggctgg tctgcctccg cctaccagga
      961 tactcgcacc cccctggcga gcgacatcca tatagccatg caccctgtcc caatgatcag
     1021 aatgtatcaa aggaccaacc tcggtgcgcg ggtcggttgg atcacccacg cgaatattgc
     1081 gcacgcgcgc ttccaggaga gcgagaaagt catcgtagac cgatttttgc aagagcaagc
     1141 gagaacccgc cgtacaacgc tcgccgttca gggaatacac ctgccagata gccccatcca
     1201 gagcccgttc caggtcagca tcatcaaaga tgatagttgg cgattttcca cccagctcca
     1261 tcgagtatcg tttcaatgtg gcggcgccat tgcgcatgat ctcttttcca gtgctggttt
     1321 caccggtgaa ggagatgagc tgtaccccag ggtgggctac cagcgacgcg ccggcggtct
     1381 ctcca
//
LOCUS       DBNHOH010000345         3021 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_404282, whole genome shotgun sequence.
ACCESSION   DBNHOH010000345 DBNHOH010000000
VERSION     DBNHOH010000345.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_404282"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="AC16V7_06190"
     CDS             <1..485
                     /locus_tag="AC16V7_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR6256744.1"
                     /translation="VISTRPVAETVPSSHEAVSVLGLKGLLTNFARCCNPAPGDDIVG
                     YITRGRGATIHRQDCPNILRVQDRERLVRVTWGEARNTYPVPVRVKAYDRDGLMKDVA
                     TLIADEGVNMTGINVDVNRNLAILDLTLEVPDVAKLSRVLDRLENLPNVMAAQRVKPG
                     "
     gene            789..2033
                     /locus_tag="AC16V7_06195"
     CDS             789..2033
                     /locus_tag="AC16V7_06195"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF015418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HIR6256745.1"
                     /translation="MSDLLSVSEALQRLLSAFSSLEIETVSITKAVGRVNAGSVQATF
                     DIPPFDNSSMDGFAVRAEDISMSSSDNGVVLNVVADIPAGQVSDVLLGKGEAARIMTG
                     APLPAGADAVVPVEDTDFNDRQPGTAAPPKVLVYRAVNSGENVRPRGQDVAAGEIVLT
                     AGQRLRPQDIGFFAMLGIPHINVYRLPRIAIFSSGDELIPVSTNLAPGKIYDSNTYTL
                     IGQVEKYGAHPVNYGIVPDNIEAVRACLDRAVAEQVDLILSSAGVSVGAFDFVKHVVE
                     QGGSLGFWRVNMRPGKPLAFGEYKGIPYIGLPGNPVSAFVGFEVFVRPSILKMSGVAD
                     FTRPVTKVRILEAIESDGRESYLRGVVTHKLGGWEARLTGHQGSGNLRSLVQANALLL
                     VPSGVKSLPIGAEVDAWILDDL"
     gene            2055..2516
                     /locus_tag="AC16V7_06200"
     CDS             2055..2516
                     /locus_tag="AC16V7_06200"
                     /inference="COORDINATES: protein motif:HMM:NF019499.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HIR6256746.1"
                     /translation="MITDSKLRSAAIVVSAIGAIDSLYLSWVKITHSQAFCGTSGQCE
                     TVNNSPYSEIAGIPIAYLGLGAYLVVIGLLYLEKREGLWRDNAPLLVFGISLVGVIYS
                     AYLTYLEIAVIHAICPYCVVSAIMMLFLLIISIIRLVQSQTTLNPVQNGGG"
     gene            2518..2751
                     /locus_tag="AC16V7_06205"
     CDS             2518..2751
                     /locus_tag="AC16V7_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256747.1"
                     /translation="MPRIRCHYMDCVLLDDGYCGAAAIELDPDMGCMTYKRSDDLVEE
                     DDWDAEEEEELDEWEELDGEDEDDELWIDDDEF"
BASE COUNT          754 a          584 c          816 g          867 t
ORIGIN      
        1 atgtcatttc aactcgtcca gttgcagaaa ctgtcccttc atctcatgag gctgtcagtg
       61 ttttaggatt gaagggcttg ctgaccaatt tcgcacggtg ttgtaaccca gctccaggtg
      121 atgatattgt gggttacatc accagagggc gaggggcgac gattcacagg caggattgcc
      181 caaatatctt gcgcgtccaa gatcgggagc ggctagttcg tgttacctgg ggtgaagcga
      241 ggaataccta cccggtgcca gtgagagtga aagcctatga ccgtgatggc ttgatgaagg
      301 atgtggctac attgattgcc gatgaagggg tcaatatgac cggcataaat gtagatgtaa
      361 ataggaatct ggcaattttg gatttaactc tcgaggttcc cgacgtagcc aaattgagcc
      421 gggtactcga tcgcctggaa aatttaccca atgtgatggc tgcccagcgt gtcaaacctg
      481 ggtaaaacat caccaaggat atcaatttgc gtgatgtgca taccctttat gtgccttggg
      541 ggctagaaat ggggcgcagt gcaaattagc ctaaatctga taaatcatta aaaaagcctt
      601 ctattttgtt tatagcaatg ctttattttg ccatctacaa tctcacggat aattgatgag
      661 tttgcctaat aaaaatgttt gtgtttatta aggcacaata gttcaatttt tattcaaggg
      721 aatggtataa ttttgcgccg cgcccattcg atgaatgtgc gtaagtcaaa gaatttcttt
      781 tatatcgtat gagcgatttg ctgagtgtct ccgaagcact tcaaagactt ttgtcggctt
      841 tttcatcact tgagattgaa acagtctcca taacaaaagc ggttgggagg gtgaatgcgg
      901 ggtcggttca agcgactttt gatatccctc cttttgataa ctccagtatg gatggctttg
      961 ccgttcgggc agaagatatc tcgatgagct cttctgataa tggagtggtg ttgaatgttg
     1021 tggcagatat cccagctggt caggtttcag atgtcttatt aggcaaggga gaagccgcca
     1081 gaataatgac aggggcgcct ttgccggcag gtgcagatgc cgtggttcct gtggaggaca
     1141 ctgatttcaa cgaccggcag cctggcaccg cagcgcctcc taaagtattg gtatatcgag
     1201 cggttaattc tggagagaac gtaagaccaa gagggcaaga tgttgctgca ggggaaattg
     1261 tactaacggc tgggcaacgc ctgcgacctc aagacattgg cttttttgca atgttgggaa
     1321 taccgcatat taatgtgtat cgtctaccca ggattgctat tttttcttca ggagatgagt
     1381 taattccagt cagcaccaac cttgcgccgg gtaaaattta tgattcgaat acatacacct
     1441 tgatcggtca agttgagaaa tatggtgccc atccggtaaa ctatgggatt gtgcccgata
     1501 atattgaggc tgtaagggct tgcctggacc gcgctgtggc cgagcaagtg gatctgatac
     1561 tatcgtctgc tggggtaagc gtgggtgcat ttgattttgt aaagcacgtc gtagagcagg
     1621 gaggaagtct gggtttctgg cgtgtcaata tgcgtcccgg caaaccactt gcattcggag
     1681 aatataaggg gattccatat atcggccttc ctggtaatcc agtctcagcg tttgtagggt
     1741 tcgaagtatt tgtgcgaccg tcaattctaa aaatgtctgg tgttgccgat tttactcgac
     1801 ccgtaaccaa ggtaagaatt ttggaagcga ttgagtccga tggtcgcgag agttatctcc
     1861 gaggagtggt tacccataag ttgggcggct gggaggcgcg attgacaggc caccaaggat
     1921 cgggaaatct gcgctctttg gtgcaagcaa acgctttgct tctggtgcca tctggggtaa
     1981 aatcactgcc gattggcgca gaagtggacg cctggatatt ggatgatctc tagagaggtg
     2041 accgtcaaat tactatgatc acagatagca aattgcgctc agctgcaatt gttgtttcgg
     2101 cgattggggc tattgattcc ctatacttga gttgggtaaa gatcacacat agccaggcct
     2161 tttgcggcac atctgggcaa tgtgagaccg tgaataacag tccctattcg gaaatagcag
     2221 gcatacctat cgcctacctt ggattaggtg catacctggt tgtaattgga ctgctttatt
     2281 tggagaaacg agagggattg tggcgtgaca atgctcccct tctagtgttt gggataagcc
     2341 tggttggtgt tatttattct gcctatctta catacctaga gattgctgtc attcacgcta
     2401 tttgccctta ttgcgttgtc tcggcgatca tgatgttatt cttgttaatt atctcaataa
     2461 ttcgtctggt gcaatctcag actacattaa accctgttca aaatggagga ggttgaaatg
     2521 ccccgtattc gctgtcatta catggattgt gtgttattag atgatggata ctgcggtgct
     2581 gctgcaatcg aattagatcc ggatatgggc tgtatgacat acaaacgctc ggatgacctt
     2641 gttgaggagg atgattggga tgcagaggag gaagaagagc tggatgaatg ggaagaatta
     2701 gatggcgaag atgaagacga tgaattatgg attgacgatg acgagttcta ggatgactat
     2761 cgtgtgggtg cgctttcctt gaaatttgca atgttaaaca tactttttat ttttaatgga
     2821 gccttctgtc aattggaagg ctcatttaat ttgagatgat tgaagcagtt gtatttggga
     2881 acataaccct cgatataatt tgcaaaacag ttaacgatgt ccccagacat gattctctgg
     2941 cttttgagga ggcggcagta atgccggggg gctgcggttc aaacgttgat ttggggctat
     3001 gtgcgctggg cgtgccaaca g
//
LOCUS       DBNHOH010000346         4525 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_212131, whole genome shotgun sequence.
ACCESSION   DBNHOH010000346 DBNHOH010000000
VERSION     DBNHOH010000346.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_212131"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..1000)
                     /locus_tag="AC16V7_06210"
     CDS             complement(104..1000)
                     /locus_tag="AC16V7_06210"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6256748.1"
                     /translation="MHLPSWNVLIQVTIIGILTGGVYALMATGLTLIFGVMRVINIAH
                     GAFLIFSAYISYWMFTKYGLDPFLSVIVSVPILFLIGIIFQRYVLSRVKGDPGLGVLL
                     TFAMALTLEGVMGSLWESTGRSIRTDYTSQVFAIHLSELTLRLPWMRVGGLVAAIAAL
                     GAIFLLLRSTNLGRAIRATIQNRSAAQLMGVNVERVQALTFGIGLASVAAGGALFSMI
                     WTFNSGSHEEWISKMLSIIVLGGMGSLPGTFVAAIIMGVAESVAAVVMTAYLSPIVFY
                     LILFVTLIFRPQGLFGVRIREG"
     gene            complement(1160..2473)
                     /locus_tag="AC16V7_06215"
     CDS             complement(1160..2473)
                     /locus_tag="AC16V7_06215"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR6256749.1"
                     /translation="MKSKFWKLLISITLMSMLIVACGGQPATEAPAPEAPAATEAPAP
                     TEVPPTEAPPPTEVPPTEEPEAEPIRIGASLPLTGRMSEPGTAAQQGYEVWAAIVNES
                     GGILGRQVELTIVDNASDQDTAVADYEKLITVDKVDLVVGPFSSFLVIPTSEVAARYG
                     YAFVEPAGGAPDVFNRGLTNIFFAQPAPGNQQAIPFTDYILALPDDQRPTTFAVVSQD
                     DPFTLGVMDGVKETLTGAGLELVFDEIYAPETTDFSSIAIQVADLDPDLIVGGTQLED
                     AVGQIRAYQEAGYQPRGAYFTTGPSLPGPFREGVGTATEGVFASISWFPEADEYQNAD
                     MVAKYTEMFGGNAGDIPEDVANAFTVGQVLHQAVENIDSLDNAALIEELHKGTYQTVV
                     GPLSFDEVGRPQGTFMLLQWQSDTFVIVGPGDRARADAIWPKPNW"
     gene            complement(2615..3955)
                     /locus_tag="AC16V7_06220"
     CDS             complement(2615..3955)
                     /locus_tag="AC16V7_06220"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HIR6256750.1"
                     /translation="MATNVIMPALEMAQESGKLVRWLKREGETVTKGEPLMEIETDKV
                     TIEIEAPDSGILGGVLIKENDEVPVGQTIAWILAPGEKVPSIPVSEPQSGRVNTLQAL
                     PETKPQPTSAPVALEISPVARKMAEQHGIDLTQFKAKGKRIEKADIQAYLNAMQPAPA
                     RSSSTVAPTSPIVLASPKARRLASERGIDLSVLTGSGPDGAVLATDVPLSVERFSAAS
                     TSLETPGTVWRLMAERMSTSWTTVPHFYLVREVDASMLVDWRKSVTPTIEKRIGIKPT
                     FTDLLVKLIAFTLREHPRLNASWADGSIQWNQEINVGIAVAIDEGLIVPVIHGADGAS
                     LSEIATRRFDLIERARQKKLLPADISGGTFTLTNLGMYNIDAFDAIVNTPQAAILSVG
                     RIADRVVPVDRQVAIRPMMVMTLSLDHRVVDGARAAQFLDDLANLIEHPWGLMS"
     gene            complement(3956..>4525)
                     /locus_tag="AC16V7_06225"
     CDS             complement(3956..>4525)
                     /locus_tag="AC16V7_06225"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR6256751.1"
                     /translation="DPVYYDILQSYMGYRTCYYRCFTIGYASHAMVDAYKRCRDYLDA
                     AIELVRPGRTTGEIASIWPKATEFGFPNEEAAFALQYGHGVGLAIWEKPVISRLVSLD
                     HPAEIKPGMVFALETYWPSSDGWSAARIEEEVVVTETGHEIITRFPANDLMVAGHHYY
                     TATGPLSGIRTHEPEMSMSVVELVESARG"
BASE COUNT         1080 a         1221 c         1208 g         1016 t
ORIGIN      
        1 gaatcccaaa acatgatagt agggcgctcg gaacagccag gggtacgcga gaagaatcaa
       61 caagacagca tatttccacc aacgagccag gaaatccgtc atctcaaccc tccctgatcc
      121 gtacgccgaa caaaccttgc gggcggaata tcagtgtaac gaacaagatt aagtaaaaaa
      181 caatcggtga gagataggct gtcataacta cagcagccac actctcagcg acgcccataa
      241 taatcgcagc cacaaatgtc cccggaaggc tacccatgcc accaagcaca atgatcgaaa
      301 gcattttcga gatccactct tcatgactgc cagagttgaa ggtccagatc atactaaata
      361 gcgcgccacc tgccgctacg gaggccaggc caatcccaaa ggtgagtgcc tgaactcttt
      421 ccacgtttac ccccattaat tgtgcggccg agcgattctg tatcgttgcg cggatagcgc
      481 gaccaaggtt agtggaacgc aataagagaa aaatcgctcc aagtgcggca atggcagcaa
      541 ccaatccacc cacgcgcatc caggggaggc gcaaagtcag ttcggaaagg tggattgcaa
      601 acacttgact ggtgtagtct gtccggatgg agcgtccagt cgactcccac aaagaaccca
      661 tcaccccttc cagtgtcagt gccatggcaa aggtgagcaa aacgcctagg cctggatcac
      721 ccttgacccg agaaagcaca tagcgttgaa agatgattcc aattaaaaat aaaattggca
      781 cagacacaat gaccgaaaga aaaggatcca gaccatactt tgtaaacatc caataggaaa
      841 tgtaagcgct aaagatcaag aatgcaccat gagcgatgtt gatcacgcgc atcacaccga
      901 aaatcaacgt cagaccggta gccatcaggg cgtaaacccc tcccgtcagg attccgatga
      961 tcgttacctg gatgagtaca ttccaagaag gaagatgcat ttgctctctc ctagataaag
     1021 ttggggctgt ctcaaaagaa ctgaacgaac agccagtgct cctgcctgta ggtcgggtta
     1081 gtatcccgac ctacaggcgg aggtttggca aatcgtccta caacttatag acagccccaa
     1141 tcgacggtca aactataatt taccagttgg gcttcggcca gatagcatct gcacgggcgc
     1201 gatcgccagg gccgacgatc acgaaggtat cactctgcca ctggagtaac atgaaggtac
     1261 cttgcgggcg gccgacttca tcaaagctga gcggaccaac cacggtctgg tacgttccct
     1321 tgtggagctc ttcgatcaga gccgcgttat ccagggagtc gatattttcg acagcctggt
     1381 gtagcacctg tccgacagtg aaggcattag caacatcttc ggggatatca ccagcgttac
     1441 cgccaaacat ttcggtgtac ttggctacca tgtctgcatt ctgatactca tctgcctcgg
     1501 ggaaccagga aatggaggcg aatacgcctt ccgttgcagt gcccacaccc tcacggaatg
     1561 ggccaggcag tgacggccca gtggtgaagt aggcgccgcg cggctgatag ccagcttcct
     1621 ggtaggcgcg gatttgccca acagcatctt caagctgcgt gccgccaacg atcaagtctg
     1681 gatcgagatc ggcaacctga atggcgatgg atgaaaagtc agtggtttca ggagcataga
     1741 tctcatcaaa gaccaactcg aggccggcac cagtcagggt ttcttttacg ccatccatca
     1801 cgcccagtgt gaagggatca tcctggctga ccacagcaaa ggtcgtcggg cgctggtcgt
     1861 caggcaatgc cagaatatag tcggtgaagg ggatggcctg ctggtttcca ggagcgggct
     1921 gggcaaagaa gatattggtc aagccgcggt taaagacatc gggggcgccg cctgccggct
     1981 caacaaaggc atagccatag cgtgccgcca cttctgaggt cgggataacc aggaagctcg
     2041 agaatggacc gacaaccagg tctactttat cgacggtgat cagtttttca tagtcagcaa
     2101 ccgcagtatc ctggtcggag gcgttatcca caattgtcaa ttcgacctgg cgaccaagaa
     2161 tgccgccgga ttcattgaca atagctgccc atacttcata gccttgttgg gctgccgtgc
     2221 ctggctcgct catccggccg gttagaggca gagatgcacc gatcctaatc ggttcagctt
     2281 ccggttcttc tgttggggga acttcggtcg gaggcggcgc ttcggttggg ggaacttcgg
     2341 tcggtgccgg tgcttcagta gctgccggtg cttcgggggc gggcgcttca gttgcaggct
     2401 gtcctccgca agctacgatc agcatgctca tgagtgtgat actgatcaac aatttccaga
     2461 atttggattt cattttttct ctcctttgga tatgattaga ttttgagttt ataagctagt
     2521 caggttatcg atgagttaat ccatccactc ctccttccat acttgcgata ttcgaggata
     2581 gcagcatgag attgtgccca ccacgtgagg tatttcaact cattagtccc cacgggtgtt
     2641 caatcagatt ggccaggtcg tccaggaatt gcgccgccct ggccccgtcc actacacgat
     2701 gatcgagtga tagtgtcatc accatcattg gccggattgc cacttgcctg tccaccggca
     2761 caacccgatc agcgatgcgt cccactgata gaatggcggc ttgcggtgta ttgacgatgg
     2821 catcaaaggc atcgatgttg tacatgccca agttggtcaa tgtaaaagtg ccacccgaga
     2881 tgtcagcagg cagcagcttt ttctggcggg cgcgttcgat cagatcaaag cgccgtgtgg
     2941 cgatctcact taatgaggca ccatctgctc cgtggatcac tgggacgatc aatccctcat
     3001 caatagcaac agcaatgcca acgttgattt cctgattcca ctggatgctg ccatctgccc
     3061 aagatgcgtt cagacgggga tgctcgcgca gggtgaacgc gatcaacttc accagtagat
     3121 ctgtaaatgt tggtttgatt ccaatgcgtt tttcaatagt cggcgtgaca ctttttcgcc
     3181 aatcaaccag catgctggca tccacttcgc ggaccaggta gaaatgtggt acggttgtcc
     3241 aactggtgga catacgctct gccatcaggc gccagaccgt tcccggcgtt tcgagacttg
     3301 ttgatgcagc cgagaatctt tcgacgctca acggcacgtc ggtggccaaa actgcgccgt
     3361 ccggcccgga gccggtcaat actgacaggt cgatgccgcg ctcactggcc agcctgcgcg
     3421 cttttggaga agcaaggacg atgggcgaag tgggtgcaac ggtagatgaa ctgcgagcag
     3481 gagcaggttg catggcgttc aggtaagcct ggatgtcggc tttttcgatt cgtttccctt
     3541 tagccttaaa ctgtgtcagg tcgatcccat gctgctctgc cattttgcgc gccactggtg
     3601 aaatttctaa cgcgacaggc gcggatgtgg gttgtggttt ggtttcaggg agagcttgca
     3661 gggtgttgac ccgcccagac tgtggttcgc tcactggtat ggatggcact ttctcaccag
     3721 gtgccaggat ccaggcaatg gtttgaccaa ccggcacctc gtcgttctcc ttgataagca
     3781 ccccacctag aatgcctgaa tctggcgcct cgatctcgat agtcacctta tcggtctcga
     3841 tttccataag gggctcacct ttggtgacgg tttctccttc acgcttgagc catctcacca
     3901 gtttgcccga ctcttgagcc atttcaaggg cgggcattat cacattggtt gccatctagc
     3961 ctcttgccga ctctaccagt tccacaacac tcatgctcat ctccggctcg tgggtgcgta
     4021 ttccagacag ggggccggtc gccgtgtaat agtgatgccc ggctaccatc aagtcattgg
     4081 ctgggaagcg ggtgatgatc tcgtgaccgg tctcggtaac cacgacttct tcctcaatac
     4141 gggccgcgct ccagccgtct gaggatggcc agtaggtttc gagcgcaaac accatacctg
     4201 gcttaatttc agcaggatgg tccaatgaga cgaggcggct gataaccggc ttctcccaga
     4261 tggccagccc gacgccatgt ccgtactgca aggcgaaggc agcctcctcg ttcgggaatc
     4321 caaattcagt agctttcggc caaattgacg caatctcgcc tgtggtgcgt ccaggtcgga
     4381 cgagttcgat agccgcatcg agataatcgc ggcagcgttt gtatgcgtct accatggcat
     4441 gcgaggcata gccaattgtg aaacagcgat agtagcaagt gcggtagccc atgtaagact
     4501 gcaaaatatc atagtagacc gggtc
//
LOCUS       DBNHOH010000347         2852 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_981769, whole genome shotgun sequence.
ACCESSION   DBNHOH010000347 DBNHOH010000000
VERSION     DBNHOH010000347.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_981769"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="AC16V7_06230"
     CDS             complement(<1..449)
                     /locus_tag="AC16V7_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR6256752.1"
                     /translation="MTYLTHLECTQCQNRFDAHKVHTFCPECTAPLFTRYDLEKAREE
                     LDRERIQRSPRGMWRWKDLLPVFEPSNIVSLGEGDTPLLNIPRLGEQLALPHLYVKDE
                     SLNPTVTFKARGLSAAVSKANEYGLNKVIIPTAGNAGGAMAAYAARGG"
     gene            791..1489
                     /locus_tag="AC16V7_06235"
     CDS             791..1489
                     /locus_tag="AC16V7_06235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR6256753.1"
                     /translation="MKVSAVMPCYNEIRTIDTIIKKIQAVELEGIEKEIVIVDDGSLD
                     GTRDYLATLQGVDGFKVVLQPENRGKGAALRTGFEHASGDIIIIQDADLEYEPNEYPK
                     LLKPILDGKADVVYGSRFVGSDTHRVLYFWHSLGNRFLTLLSNIFTNLNLTDMEVCYK
                     VFRREILNNIELKEDRFGFEPEFTAKIASGGYIVYEVGISYYGRTYEEGKKINWKDGI
                     RAVYVILKYGIFRK"
     gene            1597..2415
                     /locus_tag="AC16V7_06240"
     CDS             1597..2415
                     /locus_tag="AC16V7_06240"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007464952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR6256754.1"
                     /translation="MSARRDFLSRRWLGILFILFVAAIIILAFARLRASNLLETPQNT
                     PEIEQDPYSSERLDMVSQTIEARGIKNPTVLEAMRSVPRHHFVLEEFLDQAYADHPLP
                     IGYGQTISQPYIVAWMTELLDLKPGDRVLEIGTGSGYQAAVLAALGGIEVYSIEIIPE
                     LAESAAERLETLGYTDVKVKQGDGYYGWPEYAPFDAIIVTAAPDHLPSPLAAQLAENG
                     RLVIPIGPPGYFQSLWKFYLEDNQLKAVNLGGVAFVPFTGPGVEAQAETPSPIP"
     gene            complement(2363..>2852)
                     /locus_tag="AC16V7_06245"
     CDS             complement(2363..>2852)
                     /locus_tag="AC16V7_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256755.1"
                     /translation="ILFLGNPAYAMTTVLFTLLLFSGLGSQFSHRIPIRLGIGTLVIL
                     VVILPYFLPLLFRETLGFSIAAKLLISCLVLSPVGFLMGIPFPAGLRLISRLVHSEAW
                     IAWGWAVNGASSVMASILAALFALSWGFSWVFYLGAACYAGAWLTGSAMASLPVLPPP
                     DR"
BASE COUNT          734 a          665 c          731 g          722 t
ORIGIN      
        1 ccgcctctcg ccgcataagc cgccattgcc cctcctgcgt tgccagcagt ggggatgatg
       61 actttattca gtccatactc gttggctttg gataccgccg ctgaaagacc gcgggctttg
      121 aacgtcaccg taggattcag gctctcgtct ttcacgtata gatgagggag tgcgagttgc
      181 tcacccagcc tgggtatatt gagcaggggg gtgtccccct ctcccaagct gacaatattt
      241 gaaggctcga ataccggcaa gagatccttc caccgccaca tgcctcgcgg cgagcgctgg
      301 atgcgctcac ggtccagctc ctcgcgtgcc ttttctagat catagcgagt aaatagcggt
      361 gctgtgcact cgggacaaaa ggtatgcact ttgtgagcgt caaaccggtt ctggcactgc
      421 gtgcattcta ggtgggtcag ataggtcata gatcaataaa taaaacctgc gaaatgcaga
      481 tgatatggga gactttatct ccactcagta taaagtgaga tgattatatc cttttgtggt
      541 aggttacact gatatactct tagaccttgt tggctgaaat cttggcttta atcttcagct
      601 cgtgtatacc ctcatagggt aatttggcgt ataatgacca ggcccgctct cctgaccctg
      661 ttgcaatccg aattgacgca atgcgaacaa tcaaactacg ggtggagcaa gatattatcc
      721 agtttgggca atttaatctg aatcggtctg aagaaggatt gcgtaccaat tccatttttg
      781 aggtttttgt atgaaagttt ctgcagttat gccttgttac aatgagatca ggaccattga
      841 tacgatcata aaaaagattc aggctgtaga acttgaaggg attgaaaaag aaatcgttat
      901 cgttgatgac ggctcactag atggaacacg cgattatctt gctaccttgc agggtgtgga
      961 tggatttaag gtggttctcc aaccggaaaa tcgtggcaaa ggagccgcat tgcgcaccgg
     1021 attcgaacat gccagcgggg atattatcat tattcaggat gccgacctgg aatacgaacc
     1081 gaacgaatac cccaaattat tgaaacctat tttggacgga aaagcggatg tcgtctacgg
     1141 ctcccgcttt gtcggcagtg acacacaccg ggtcttgtat ttctggcatt ccttaggaaa
     1201 ccgattcctc actttgctct cgaacatatt tacgaacctg aacttgaccg atatggaggt
     1261 ctgttataag gtattcaggc gcgagatcct caacaatatt gaattaaaag aagatcgctt
     1321 cggatttgaa ccggagttca ctgcgaagat agccagcggt gggtatattg tctatgaagt
     1381 aggtatctct tattatggtc gcacctacga agaggggaaa aagatcaatt ggaaagatgg
     1441 catccgagcg gtttacgtga ttctcaaata tgggatattt cgtaaataat cagaattccc
     1501 acccttaatc taataatatg cagttgccat gtcaccctag cggtttgctg gggtgatttt
     1561 tttacgcggt gtataatcaa gtagggagtg aattcgatgt ctgctcgaag agatttctta
     1621 tcacgccgtt ggctggggat cctgttcatc cttttcgttg ctgcaatcat tatccttgcc
     1681 tttgcgcgct tgcgggcgtc taatcttctt gaaactccac agaacacccc agaaattgag
     1741 caagacccct attcatctga gcgcctggat atggtaagcc aaacgattga ggcgcgtgga
     1801 attaaaaatc ctacggtgct ggaggccatg cgcagtgtgc ctcgccatca ttttgtcctt
     1861 gaagagttcc ttgatcaagc ctatgcagac cacccattgc cgattgggta tggacagacc
     1921 atctctcagc catatatcgt ggcttggatg actgagctgc tcgatctgaa gccgggggat
     1981 cgggtgctgg agataggcac gggctcggga taccaagctg cggtactagc tgctctgggt
     2041 ggaatcgagg tttattccat tgagataatc ccggagttgg ccgagagcgc cgctgagcgg
     2101 ttagaaacac taggttatac ggatgtgaaa gtcaagcagg gagacggtta ttatggctgg
     2161 cctgaatatg ccccatttga tgccattatc gtcactgcag ctcctgacca cctgccctcc
     2221 ccattagcag cccaactggc tgagaatggg cgtctggtca ttcctatcgg cccaccggga
     2281 tattttcagt cgctgtggaa attctattta gaagataacc aattgaaggc agtaaatctg
     2341 ggtggggtag cgtttgttcc gttcaccggt ccgggggtgg aagcacaggc agagacgcca
     2401 tcgccgatcc cgtaagccac gcacctgcgt agcaagctgc acccagatag aatacccagc
     2461 taaaacccca agagagcgca aataaggctg ccagaatgga cgccataacc gaagatgcgc
     2521 catttacagc ccatccccag gcaatccatg cctcgctgtg aaccaatctt gagatcagtc
     2581 gtaatccggc agggaaaggg atgcccatca gaaatcccac tggtgataat acgagacaac
     2641 tgatgagcag tttcgctgcg atcgaaaagc ccagtgtctc tctgaatagc aggggcaaga
     2701 agtatggaag aatgacgacc aagatcacca gcgttcctat cccaagacgg attgggattc
     2761 tgtggctgaa ttgactaccc aagccagaga acaataataa tgtgaagagg actgtggtca
     2821 tggcgtatgc cgggttgcct aagaacaaga ta
//
LOCUS       DBNHOH010000348         2385 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_853339, whole genome shotgun sequence.
ACCESSION   DBNHOH010000348 DBNHOH010000000
VERSION     DBNHOH010000348.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_853339"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="AC16V7_06250"
     CDS             complement(<1..317)
                     /locus_tag="AC16V7_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6256756.1"
                     /translation="MKLDFHPVTPDRWSDLETLFGPRGATGGCWCMYWRLKRSEFEQM
                     KGEGNRQAMKSIVDSGEIPGLLAYHDGQPVGWISVAPRPAFSALERSRILKPVDERPV
                     WSVV"
     gene            complement(446..1345)
                     /locus_tag="AC16V7_06255"
     CDS             complement(446..1345)
                     /locus_tag="AC16V7_06255"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6256757.1"
                     /translation="MTTPFRIDPRIRAYGKARIDVTRRARDEREWQTQWREPRYPFPF
                     SWEGGWRHCIQYTVDDFAAEIGFYIDVLGFPVNAFSPSYAQFTSPDQAFFFAVAAIQE
                     GAQSTPFDSLRMQFMVEDLPATIEELEKRGISFERKPESDEVFPAIAILRSPHGIGIE
                     LWGVVRDQPRDATHEMSEEDDTADFWADDDEVEDEVDEQEADDTQDDAEQPSYEGIGD
                     GELVDEPVYVDEDEAGQKEPLHPMVANPTDGSSRSIPSLPRAVIRRTEDLLKGGVQFP
                     VRKVRGNGFRNQKLLDVNGDPGE"
     gene            complement(1386..1751)
                     /locus_tag="AC16V7_06260"
                     /pseudo
     CDS             complement(1386..1751)
                     /locus_tag="AC16V7_06260"
                     /inference="COORDINATES: protein motif:HMM:NF013222.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
     gene            1912..2100
                     /locus_tag="AC16V7_06265"
     CDS             1912..2100
                     /locus_tag="AC16V7_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256758.1"
                     /translation="MTLSTPKTQTAQVAMAMTQGSTISPYNLQMAASLLTVAVPLMIF
                     LFLGRYFIRGILAGVLKG"
BASE COUNT          565 a          643 c          560 g          617 t
ORIGIN      
        1 acaaccgacc agactggcct ttcatctacc ggcttgagga tgcgcgagcg ttccagggcg
       61 gaaaatgcag ggcgcggcgc aacagagatc cagcccaccg gctgaccatc atggtaggct
      121 agcagaccgg gtatttcgcc cgagtccact atggacttca tggcttggcg gtttccctcg
      181 cccttcattt gctcgaattc agagcgcttc aagcgccagt acatgcacca gcaacctcca
      241 gtggcacccc ttggcccgaa cagggtctcc aggtcagacc agcggtcggg ggtgacggga
      301 tgaaagtcta atttcatggg tggggagcct cctatcgaaa cttgattcta cctctaatgc
      361 tgaagtgctg ggattccatc agccgcccgg ttctcccgag cggctgtctg gatatatatc
      421 attttgaaat tacttgacga tgctactact ctcctgggtc gccattcacg tcaagtagct
      481 tttgattacg gaagccattc ccacgcacct ttcgaactgg aaattgaacg ccgcctttga
      541 gcaggtcttc cgtacggcga atgactgccc gaggcaagga tgggatggaa cgtgatgatc
      601 catctgtcgg attagcaacc attgggtgaa gaggctcctt ttgtcctgct tcatcctcat
      661 ccacatacac tggctcatct actaattcac catcgccaat accctcatag gatggctgtt
      721 ccgcatcatc ctgggtatca tctgcttcct gttcatctac ttcgtcttcc acctcatcat
      781 catccgccca aaaatcggca gtatcgtcct cttcgctcat ctcatgggtg gcatcacgtg
      841 gctgatcacg aaccacaccc cataattcga tcccgatgcc gtgtggagaa cgcaaaatgg
      901 caatggcagg aaaaacttca tccgattccg gtttgcgctc aaagctgata cctcgcttct
      961 ccaactcctc gatcgtagcg ggcaagtctt ccaccatgaa ctgcatgcgg agcgaatcga
     1021 acggcgtgct ctgagcacct tcctggatag cggccacagc aaagaaaaaa gcttgatccg
     1081 ggctggtaaa ttgggcatag gagggggaga aggcattgac tggaaatcct agtacgtcaa
     1141 tgtagaaacc gatttcagcg gcgaaatcgt caacagtata ttggatgcaa tgcctccaac
     1201 cgccttccca actaaacggg aacgggtagc gaggctctcg ccactgcgtc tgccactcac
     1261 gctcatcgcg cgcacgccga gtgacatcga tgcgcgcctt gccataggct ctaatacgag
     1321 gatctattcg aaatggtgtt gtcatgtcat ccatctcctg gaaataccaa ccaataggct
     1381 tgaagttatt cttcgggtga cccagcccac ccaaatatct tcaaatcaac acggtcgcgt
     1441 gaatcgaata caacaccttc agcttccaat aactggcgct gctgcaaggc actgcttgag
     1501 gggcgcaggc tgattttgcc ttgagaattg atcacccgtt gccaggggac atcttctggt
     1561 caagctgcca tagctccccc cacccagcgg gcagaccagg cggcgtaatc ctttggatcc
     1621 atacccttcg gaggagggat aagcgcggcg atattcccat aggtgattac tctaccccca
     1681 gggacctggc gaacgatatc ccacacttta ttacgaaaat cagcttgctg tgctggagac
     1741 aggtatttca ttcttcaggt ctccttgtga tctcacttaa tgtgaattgt ctcaaaacta
     1801 atcctgattg catcgattat ttatatcgaa tagaacgaca aaagatctgt atcagtctct
     1861 gcagttaaat atacacgaac gtttgttcta tgtcaagatc atttcatatt tttgacgctg
     1921 agcactccaa aaacgcagac cgcccaagtt gccatggcga tgacccaagg gagcacgatt
     1981 tcaccataca acctgcaaat ggctgcttct cttttaaccg tcgccgtgcc cctgatgatt
     2041 ttcctgttcc tgggtcgtta cttcatacgc ggcatcctgg ctggggtgct aaagggttga
     2101 cattgttttt ttcgcatttc ccatttcaac cgaaaatgct ttacatcgca tatatctttc
     2161 tgtataattt aaaaaggtca caggcttagt tctacgaata ttgggggaga ccatacatct
     2221 ttgttccatc gctggcggaa tatattaagc tgcgcgacct tatctagtac ttgggtggga
     2281 tgtttatcgg tcatcttctg ggtgaggtga aaggaggtga tgtctgcgat tcagcaagct
     2341 ccatactgtt atcgactgaa tgttcatatg tatttgtctt ccttt
//
LOCUS       DBNHOH010000349         4343 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_981812, whole genome shotgun sequence.
ACCESSION   DBNHOH010000349 DBNHOH010000000
VERSION     DBNHOH010000349.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_981812"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1128)
                     /gene="mvaD"
                     /locus_tag="AC16V7_06270"
     CDS             complement(154..1128)
                     /gene="mvaD"
                     /locus_tag="AC16V7_06270"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR01240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HIR6256759.1"
                     /translation="MRSATAIANPNIAFIKYWGDRERALRLPANGSISMNLDGLYTRT
                     EVVFDPALQQDSLTLDGHSITGPGLERVSALLERVRRMAGMALHARVSSENNFPAGAG
                     LASSASAFAALSLASSAAAGLQLSEQELSRLARTGSGSACRSVPGGFVEWQMGEGDQD
                     SYAFSIASPAHWRLVDCVAIVSHEHKPTGSTEGHARAQTSPLHAARLAEVGRLLVDCR
                     HAILSRDFEALARATEMDCHLMHAVMMTSMPALIYWQPATLEVMQAVRDWREAGLPVC
                     YTIDAGPNVHVICEEQVATQVRAQLGQIDGVKQVLAAGVGGPARLEIR"
     gene            complement(1898..2923)
                     /locus_tag="AC16V7_06275"
     CDS             complement(1898..2923)
                     /locus_tag="AC16V7_06275"
                     /inference="COORDINATES: protein motif:HMM:NF016836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR6256760.1"
                     /translation="MDKEQVLQSNLGEYSRCDKHKQKTDHRTIISKTQKTDAALKEKI
                     RNALWNDKVLRALDDNEIDVQVINQVVYLEGHIVSSSSLNRIINALHTISGILGIKNY
                     LVLDDKLSSQIASSLGMLEHNNDCKFFTGVTHGVVVLNGQVNSKNLRKKAEQCAANNP
                     KVRGVINYIRVPGVELDIQDHRFLQPSIGEEIKFLDGISGTVRKVIINPDNRRVVAMI
                     IQGHFYGVMRNLPLSKLNASQSEEQIIVIPMVATRHVNERSIFLNINSSDFTKYPEFD
                     PSKFIVPNKDWLPPYPYCPDDILIPVKFQPVDKDIAGNPIQASSTHITGIPKWMEELL
                     YNDSLGG"
     gene            complement(2993..3127)
                     /locus_tag="AC16V7_06280"
     CDS             complement(2993..3127)
                     /locus_tag="AC16V7_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011715667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256761.1"
                     /translation="MAKSQKNKKDSKKKPLRTKKEKRQAKRDKKNNNNDLITSINQTN
                     "
     gene            complement(3247..3636)
                     /locus_tag="AC16V7_06285"
     CDS             complement(3247..3636)
                     /locus_tag="AC16V7_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017301671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256762.1"
                     /translation="MKKEVGLWIDHREAFLVTLTDNMEELNRIYSKIEVDAQFSGVSR
                     SAGSQEAENIQDRKYLNNLEKYYDVVIGYIRDADSILIFGPGKAKGELAKSLKNKNLS
                     QHIVSIETSDKLTEPQIAAKVREYFPR"
     gene            complement(3888..4208)
                     /locus_tag="AC16V7_06290"
     CDS             complement(3888..4208)
                     /locus_tag="AC16V7_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256763.1"
                     /translation="MNRETIGKWAFIIGLVIAVLAGLFFQPGWAIWVLAVLGVIVGLL
                     NVTAEETRGFLLASIALTISATALNSLPIIGTAFSYVLPFIVAFVAGGMIVVALKELF
                     QTAR"
BASE COUNT         1054 a         1021 c          984 g         1284 t
ORIGIN      
        1 ctgtaacaat tgattcgcaa atagattaga gacaatcatt tttctgtaaa atccatccaa
       61 aagcgttgtc tggaataaaa caacacgtaa ttttactcat tatatccttt tcgctaacaa
      121 gtcgctagat acaggggatg tgaacgagaa tttctaacga atttctaagc gcgccgggcc
      181 accaacgcca gctgccaata cttgcttgac accatcgatt tgcccaagct gtgcgcgcac
      241 ctgggtagca acctgctcct cgcagatcac gtgcacattc ggtccagcgt caatcgtgta
      301 gcagacgggc aagcccgcct cgcgccagtc acggaccgcc tgcatgacct ccaaggtagc
      361 cggctgccag tagatcaaag caggcatgga ggtcatcatc acggcatgca tgaggtggca
      421 atccatctcg gttgccctgg ccagagcctc gaagtcacga ctcaggatgg cgtgccggca
      481 atccaccagc aagcgcccga cttctgcaag gcgggcagcg tgcagcgggc tggtctgcgc
      541 acgggcatgc ccctctgtgg agccagtggg cttgtgctca tggctgacga tagccacaca
      601 atccaccaag cgccaatgcg cgggtgaggc gatcgagaag gcataggaat cctgatcccc
      661 ttcacccatc tgccattcga cgaagccccc cgggactgag cggcaggctg agccactgcc
      721 ggtgcgcgcc aggcgggaga gctcctgctc gctcagctgc agtccagcgg ctgcggatga
      781 ggccaggctt agagcggcaa acgctgaagc cgacgaagcc aacccggccc cagcagggaa
      841 gttgttttcg ctgctcaccc tggcgtgcag cgccatacct gccatgcggc gtactcgctc
      901 caaaagcgcg ctcacacgct ccagccccgg gcctgtgatc gaatgcccat cgagagtcaa
      961 ggagtcctgc tggagggcag ggtcaaaaac cacctctgtg cgggtgtata acccgtccag
     1021 gttcatggaa attgagccgt tcgccggcaa gcgcaaggca cgctcgcggt cgccccagta
     1081 tttaataaaa gcaatatttg gattcgcgat tgcagtagct gatcgcatac tgcgagttta
     1141 ccacagggtt agcaggtcgc tggcaagata caactgcacg atggctgtgt atcatgctcc
     1201 ctgatgcccg accggagcca ccttggacac cggcaccaag ggtcagctat tcagttgtat
     1261 cgaaccccct atgttcactt ctggggtgaa ctacccctct acttagggtg ctccaaaatt
     1321 tattcggatt gaaaaagaga aagagcaatt caattgaacc cccccctata ttcgctttag
     1381 ggtcgcgcta gccccttata taaccacgtg actaccggac ggggggcgat tcggtacgga
     1441 tatacgtgga tttgtgttgt gctgtgaggc ttttattgta tactaatatt agctttcaat
     1501 aacaacacta caacgtgttg cgaataatat cagggagtaa tattgctctg ttcgtcgccc
     1561 tcctaggaag ccgttggagg gcgtttgcat tagggatgga tttttattac aagtctggga
     1621 gggacagatg acttggattc aactcgtaag tgcaacaggt acattctctt taaagaactc
     1681 ttgaaaagga gaattgaagg cgagactgtg gtggacccat cattgtttgt tggggggata
     1741 tatataagcc ttattaatac cccgatgaca ccttacgcta acaggatgtt ttcggtataa
     1801 gtttacttca cgcatattca cgcatattat tttttcttca ccatgttttt tatgcgtttt
     1861 taatgttagt catcgtttaa tggtttttct ccttatacta tccgcccagg ctgtcattat
     1921 ataggagttc ttccatccat ttcgggattc cagtaatgtg tgttgatgat gcctggatcg
     1981 gattgcccgc tatatcttta tctacgggtt ggaacttaac cggaattaga atatcatcgg
     2041 gacagtaggg gtatggcggt aaccaatctt tattcgggac gatgaatttg gatgggtcaa
     2101 attctggata ctttgtgaag tctgagctat taatgtttag gaaaattgac ctttcattca
     2161 catgccttgt ggcaaccatc ggaataacaa ttatttgttc ttctgattgg ctagcgttga
     2221 gtttcgataa cgggagattt cgcattaccc cataaaagtg cccttgaatt atcatggcga
     2281 ctactcgacg gttatctggg ttgatgataa cttttctaac tgttccagaa atgccatcaa
     2341 ggaatttaat ttcctcgcca atggatggtt gtaaaaaacg gtgatcttga atatccaatt
     2401 ctaccccggg cacacgaatg taattgataa caccacggac tttggggtta ttggcggcgc
     2461 attgttctgc ttttttacgt aaattcttgc tgttcacttg accgttcagc accactacgc
     2521 catgtgtgac tcctgtgaaa aacttacagt cattattgtg ttccaacatc cctaatgatg
     2581 aagcaatttg gctggatagt ttatcatcca aaacgagata attcttaatc cctaagattc
     2641 ctgaaatggt gtgcaaggcg tttataatcc ggttcaggct ggatgaactc acaatatgtc
     2701 cttcaaggta gacaacctga tttattacct ggacatcaat ttcgttgtca tccagagctc
     2761 tgagaacctt atcattccat aaggcgttcc taattttttc ctttaatgcc gcatccgttt
     2821 tctgggtttt actaataatt gttctgtgat ctgtcttttg cttatgttta tcacaccggg
     2881 aatattcacc caaatttgat tgcagtacct gttccttatc catttgtgtt tctctcaaga
     2941 ctatcttttg gcggattttc catataaacc agcaaaggtt atatttgatt ggctaatttg
     3001 tttgattgat tgatgttatc aagtcattat tattgttttt cttatccctt ttggcttgtc
     3061 ttttttcctt tttagttctt agcggtttct tcttagaatc ctttttattc ttctgacttt
     3121 tagccatgat ttttctcctt aggtggttta tttttaggtt agattgcagc tatgaatttg
     3181 tgtttgttag ttgaaactgg gacttattct taattccgag acattcttta cttcttgtgg
     3241 caactgctat cttggaaaat actctcggac tttagccgca atttggggct ctgtgagctt
     3301 gtcactagtc tcaatgctta cgatatgttg acttaagttt ttatttttca gactttttgc
     3361 gagctcgcct ttggctttac caggtccgaa gatcaggatt gagtctgcat ctcgaatata
     3421 cccaatgacg acatcatagt atttctctag attgtttaaa tattttctgt cctgaatgtt
     3481 ttccgcttcc tgggatcctg ctgaccttga gaccccagaa aactgggcat ctacttcaat
     3541 ttttgagtag atccgattca gttcctccat attatcagtg agtgtcacga ggaacgcctc
     3601 tcgatggtca atccacaatc ccacttcttt cttcatggat atggttcctt tctttcttcc
     3661 ttgtcacatt gattggctgg ctgccttctg gcagcgttgt cgtgctacgg gtctgcgtcg
     3721 gctcagacat gtttttgtaa ggcgaattca tagagcccga agatggttgt caataaacct
     3781 ttccgaacgt caccaacacc taatacaaaa tgcgctttgc cttatcgata gcaggtggga
     3841 aatataccat tgcctgctat cgataattgg gcagttggct aggattatta tcgagcggtc
     3901 tggaatagtt ctttcaatgc gaccacaatc attccaccag cgacaaaggc tacaatgaac
     3961 ggcaggacat aggaaaaagc tgttccgata atgggtagag agtttaacgc cgttgctgaa
     4021 atagtaagtg caatcgaagc caacagaaag cctcgtgttt cttcggcggt gacattgagc
     4081 agacctacga tcacaccaag gaccgccagc acccagatag cccatccggg ttgaaagaac
     4141 agcccggcga gtacggcaat aaccaggcca atgataaaag cccatttccc gatagtttca
     4201 cgattcattt tagtctcctt tcactaacga atagcaagtc cctcttcgaa ggttatgttt
     4261 tggttcctca aagaggataa tttaacttac tggaacatta ttctcaattt atttgattcc
     4321 tttctcgctg gataagttca ccg
//
LOCUS       DBNHOH010000350         3387 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_532769, whole genome shotgun sequence.
ACCESSION   DBNHOH010000350 DBNHOH010000000
VERSION     DBNHOH010000350.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_532769"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="AC16V7_06295"
     CDS             <1..357
                     /locus_tag="AC16V7_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR6256764.1"
                     /translation="SNGSEQTRVTWNEYDDNFPVWSPTENILLYTAVINGVQEIYTMD
                     LDTGDEWELTGDTSGEDHPRWSPDGHYIVYDAKNGPGSNGGVDSEIYLFDYWSDQAIP
                     IRLTDNDVDDDAPAVK"
     gene            367..759
                     /locus_tag="AC16V7_06300"
     CDS             367..759
                     /locus_tag="AC16V7_06300"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl trahydropterin synthase family
                     protein"
                     /protein_id="HIR6256765.1"
                     /translation="MYTVAVKCDFIAQHYLIGGDWGAENQLHAHHYQAEVQLEGSELD
                     QHGYLVDIVDIENALDTLVARYRDRALNDLPEFAGLNPSIEHFSRILCQALVAHIQSA
                     NLRAVTVKLWENEIAWASYRAEDLNSRG"
     gene            808..1908
                     /locus_tag="AC16V7_06305"
     CDS             808..1908
                     /locus_tag="AC16V7_06305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6256766.1"
                     /translation="MKLGLVIYGSLDSLSGGYLYDRKLVNNLTRLGDTVDVIALPPGQ
                     PTRAGWLNYVRHLGDNLSQGLSRRLERLEVDILLQDELNHPSLAWVNRRLRARVAYPV
                     VSIVHHLRSDELRPAWQNRFYAWVERIYLKSVDGFIYNSRTTQGRVERLAGKGLPSVV
                     AYPAANHLNPQIEQVEIIQRARQAGPLSLFFVGNLIPRKGLHTVLEALSRLPKGEFIL
                     EVAGSLTSDPVYVDAIRQQIEKLNLGSAVRLHGALGDSQLVDNYRRAQVLVLPSSYEG
                     FGIAYLEGMGFGLPCIGTTAGAAGEVIEEGQTGLLIPPGDSVSLANHLRRLADDRTLL
                     ETMSLAARRRYLSHPTWEQTAEKTRSFLETLI"
     gene            1923..2831
                     /locus_tag="AC16V7_06310"
     CDS             1923..2831
                     /locus_tag="AC16V7_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6256767.1"
                     /translation="MEYSFPRYLLSKQSVDDRALNPRVYAALHSSLPYQSPLRITEVG
                     AGIGTMFTRLIHWGLLDKADYTVVDETPENIDYAREWLPRQAREAGFEVEESGNSLRL
                     YDASRDIRVHLLASDVFEFAQTQPPRAHLLIANAFLDLLPLPESLTSLFSLVEPKGLA
                     WLTINFDGVTIFEPQIDPALDAQIENLYHRSMDERLTGGQSSGDSRTGRHLFAYLRQP
                     GLGAEIIEAGSSDWVVYPHQGHYPADEAYFLHFILYFFERTLSGNPDLDPARFADWLA
                     ARRAQIEKGELIYIAHQMDFLVRVHG"
     gene            2854..3303
                     /locus_tag="AC16V7_06315"
     CDS             2854..3303
                     /locus_tag="AC16V7_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011387828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR6256768.1"
                     /translation="MFSQMISADNADGLPALPGSYALRLRLAKDSILQVGRLGQFRFS
                     RGDYVYLGSALGPGGLRARLGRYLRGDGRLRWHIDTLRTAAQVFGFHYIISPLSLECR
                     WNQALAAMPGSSTPVPGFGASDCRSICPAHLIFFSEGEIPAVFPYPD"
BASE COUNT          704 a          943 c          951 g          789 t
ORIGIN      
        1 agcaatggca gtgagcagac tcgcgtgaca tggaatgaat acgacgacaa tttcccggtc
       61 tggtctccga ccgaaaatat tttgctctat acggcggtca ttaatggtgt ccaggagatt
      121 tatacaatgg atctcgacac gggagatgag tgggaattaa ccggtgatac cagcggtgaa
      181 gatcacccgc gctggtcacc ggatgggcat tacattgtct acgatgctaa aaacggcccg
      241 ggatcaaacg gaggggtaga ttctgagatt taccttttcg attattggtc tgaccaagcc
      301 atcccgataa ggctaaccga taatgatgtg gatgacgacg cgcctgcggt aaaataagct
      361 tcagttatgt acactgttgc tgtcaagtgc gacttcatcg cccagcatta cctgatcggc
      421 ggggattggg gggcggaaaa ccagcttcac gctcatcact accaggcaga agtgcaattg
      481 gaaggctctg agctggatca gcatggctac ctggtggaca tcgtggatat cgagaatgct
      541 ttggataccc tggtcgcccg ctaccgcgac cgggcgctca acgatttgcc ggaattcgct
      601 ggtcttaacc ccagcatcga gcatttctct cgcatcttgt gccaggcgtt ggtcgcccac
      661 atccagtctg ccaatctgcg cgccgtcacg gttaagctat gggagaacga gatagcctgg
      721 gcatcctatc gggcggaaga tctcaatagt aggggctgaa gtgcccttcg atattcactc
      781 taccggtact tcaccgttta accacccatg aagctgggcc tggtcatcta cggcagcctg
      841 gattccctgt ctggcggtta cctgtatgat cgcaagctgg tcaataacct gacccgcctg
      901 ggcgatacgg tagacgttat cgccttgccc cctgggcagc ccacaagggc gggctggctt
      961 aactacgtcc gccacctggg ggataacctg tctcagggat tatcccgccg ccttgagcgc
     1021 ctggaggtcg atatcctgct gcaggatgag ctcaaccacc catccctggc gtgggttaac
     1081 aggcggctgc gcgctcgcgt ggcatacccc gtggtttcca tcgtgcacca cctgcgcagc
     1141 gatgagctcc gtcctgcctg gcagaaccgt ttctacgcct gggttgagcg catctatttg
     1201 aagagcgtgg atggattcat ttacaacagc cgcacgaccc agggcagggt ggaaagactg
     1261 gctggtaaag ggctgccgtc ggtggtcgct tacccggcgg ctaatcatct gaatcctcag
     1321 atcgagcagg ttgagatcat ccagcgtgct cgccaggcag gccctctatc cctgttcttt
     1381 gtaggcaact tgatcccgcg caaaggcttg cataccgtgc tcgaggccct ctcccggctg
     1441 ccaaaagggg agttcatcct cgaggttgcc ggcagcctga ccagcgatcc cgtctatgtg
     1501 gatgccatcc gacagcagat tgagaagcta aacctgggtt cagccgtccg cctgcatggc
     1561 gcgctgggag actcgcaatt ggtggacaat taccgccgcg cccaggtatt ggtgctgccc
     1621 tcctcctacg agggctttgg catcgcctat ttagagggta tgggctttgg tctgccctgt
     1681 atcggcacaa cggccggcgc ggctggggaa gtcatcgaag aaggtcagac cggtctcctg
     1741 atcccgcccg gggactctgt atcgctggca aaccacctgc ggagactggc agatgatcgc
     1801 acactactcg agaccatgtc cctggctgct cgccggcgct atctatccca tcctacctgg
     1861 gagcaaacag cagaaaaaac caggagtttt ttagaaacgc ttatctaatc tggaatattc
     1921 aaatggaata ctcttttcca aggtacctgc tgtccaaaca gagcgtggat gaccgcgccc
     1981 tcaaccctcg cgtctacgcc gctttgcatt ctagtctgcc gtatcaaagc ccgctgcgta
     2041 ttaccgaagt gggagcgggg atcggcacta tgttcacccg cctgatccat tggggtctgc
     2101 tcgataaggc tgactatacc gttgtcgatg agacccctga gaatatcgac tacgccagag
     2161 aatggctgcc gcgacaggcg cgggaggcgg gattcgaagt tgaagaatcg ggtaattcgt
     2221 tgcgcctgta cgatgccagc cgggatatcc gcgtgcactt acttgcctcc gatgtcttcg
     2281 aattcgccca aacccagccc cctcgggcgc atttgctcat cgccaacgcc tttcttgacc
     2341 tgctgcccct gcccgagagc ctgacgagcc tgttctcatt ggtcgagcct aagggccttg
     2401 cctggttgac gatcaatttc gacggagtga ccatatttga accgcagatt gaccctgctc
     2461 tcgacgccca gattgaaaac ctctaccacc gcagcatgga tgagcgtctc acaggagggc
     2521 aatcatcggg agacagccgc accggtcgcc acttgttcgc atacctgcgc cagcctggtc
     2581 taggcgctga gatcatcgag gcgggctcgt cagattgggt tgtatacccc catcaaggtc
     2641 actacccggc ggacgaagct tactttttac acttcatctt gtatttcttc gagcgcaccc
     2701 tcagcggaaa ccctgacctc gatccggcgc gtttcgcgga ttggctggcg gcacgccgcg
     2761 ctcaaatcga gaagggcgag cttatttaca ttgcccatca aatggatttt ctggtccggg
     2821 tccatgggta aaggggatat tggcttgaaa tcagtgttct ctcagatgat ttctgctgat
     2881 aatgcagatg gcttgcctgc tttgcctggt agctatgccc tgaggctgcg attggcaaaa
     2941 gattcgattc tacaagtggg tcgtctggga caattccgtt tttcgagagg ggattatgtc
     3001 tacctgggta gcgcgctcgg gccgggtggc ttgcgagccc gtttgggtcg ctacctgcgt
     3061 ggcgatgggc ggttgcgctg gcatatcgat accttgcgca cagctgccca ggtatttgga
     3121 tttcactata tcattagccc attatccttg gaatgccgct ggaaccaggc tctggccgca
     3181 atgccaggat ccagtacacc ggtccctgga tttggagcca gcgactgccg ttcaatttgc
     3241 cctgcccatc tgatcttctt ctcagagggc gaaattccgg cggtttttcc ctatccagat
     3301 tgattaaata agcgaagggg tggtacgaga ggagatgatt accgcttttg gttcccggaa
     3361 gggagagata aaaagcaggg atcgtgt
//
LOCUS       DBNHOH010000351         2265 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_853404, whole genome shotgun sequence.
ACCESSION   DBNHOH010000351 DBNHOH010000000
VERSION     DBNHOH010000351.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_853404"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1944)
                     /gene="lepA"
                     /locus_tag="AC16V7_06320"
     CDS             complement(145..1944)
                     /gene="lepA"
                     /locus_tag="AC16V7_06320"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIR6256769.1"
                     /translation="MSELIRNFCIIAHIDHGKSTLADRLLQLTGTISERDMTEQVLDS
                     MDLEREKGVTIKASAVRMQYTARDGLSYELNLIDTPGHVDFGYEVSRALAACEGALLI
                     VDATQGIEAQTLANLYMALEADLEIIPVINKIDLPSAHPDQVAEEIGSLLGTPAEKVL
                     RISAKAGINVESVLEAVVEEIPPPSGDDGTPLQALIFDSHYDPYKGVVAYIRLMEGCL
                     KHTDSLRLMVSDVVLKPVEIGVFTPYMRPAQELRAGEVGYVATGLKTVRECRVGDTLT
                     QVTHPAAKPLPGYRKVKPMVFAGIYPVEGEEYDDLRDALEKLQLNDASLIYEPETSQA
                     LNFGFRCGFLGMFHMEIIQERLEREYDLDIIVTAPSVEYEVILNDNRVVRIDSPAQLP
                     DESQIVEIREPWMKIQIFTPTEFYGTVMELATGRRAIFTDQEYPAPMRVQLNFEIPLS
                     ELIVDFFDQLKSRTRGYASLDYQFAEYRPDDLVKLEILVNNEPVDALATIVHRKDAFH
                     KGQAVISKLKDLIPRQLFVVPIQAYAAGRVISRANVKALRKDVLAKCYGGDITRKKKL
                     LEKQKRGKKRMKMVGNVEIPQDAFLAVLRHGED"
BASE COUNT          560 a          612 c          538 g          555 t
ORIGIN      
        1 gcttcaccca gtttttttat gtctgcgaaa tagaatacaa gacccagcga tatcaaactg
       61 atcgccaggc caggcaaaat tcgcctccac tcaccaccct gctggcgagt ttcttggggg
      121 ggttggctga cttccaatgt ggtttcaatc ctccccatgc cgcaatacag ccaggaaggc
      181 atcttgggga atctcgacgt tcccaaccat cttcatgcgt ttcttaccgc gtttttgttt
      241 ctccagcaat ttcttcttac gggtaatatc accgccataa cacttggcta atacatcctt
      301 gcggagagct ttgacattgg cacgcgagat cacccgtcca gcggcgtaag cctggattgg
      361 cacaacgaaa agttggcgcg ggattagatc tttcagtttg gagatcaccg cctgaccttt
      421 atggaaggca tccttgcgat ggacaatcgt cgccagcgca tccactggct cattgttgac
      481 caggatctcc agcttgacca aatcatcagg tcggtattca gcaaactggt aatccagcga
      541 ggcatatcca cgtgtgcgtg atttcagttg atcgaaaaag tcgacaatca attcagagag
      601 agggatttca aaattgagct gtacccgcat cggagcagga tattcctggt cggtaaagat
      661 cgcacgtcgt ccagtagcta gctccattac tgtcccatag aactcagttg gggtgaaaat
      721 ctgtatcttc atccatggtt cgcgaatttc gacaatctgg gactcatcgg gcaattgggc
      781 aggcgaatca atacgcacaa cccggttgtc attcaagatc acctcatatt ccaccgaggg
      841 cgcggtgaca atgatatcca aatcgtattc gcgctccagc cgctcttgaa tgatttccat
      901 atggaacatg cccagaaaac cgcaacgaaa gccaaaattt aacgcttgag aggtctccgg
      961 ctcatagatc aaagaagcat cgttcagttg tagcttctcc aacgcgtctc ttaagtcgtc
     1021 atactcttcg ccttccacag ggtagatgcc agcaaatacc atgggcttaa ccttgcggta
     1081 gcctggcaga ggcttggcag ctgggtgggt tacctgggtt agggtatccc ccacgcggca
     1141 ttcgcgcact gtcttcaaac cggtggcaac atagcccacc tcgccggcac gaagctcttg
     1201 ggctggacgc atatatggag taaaaacgcc gatctccacg ggtttgagca ccacgtcgct
     1261 gaccatcaac cttaagctat ctgtatgttt taggcagcct tccatcagcc ggatataggc
     1321 aactactcct ttatatgggt cataatggga gtcaaaaatc aaggcttgca atggggtgcc
     1381 gtcatcccca ctgggcgggg gaatttcctc caccaccgct tccagcaccg actcaacatt
     1441 aatcccggcc ttcgcagaga tgcgcaagac cttctctgca ggtgtcccta gcaggcttcc
     1501 aatctcttca gccacctgat caggatgggc agacggcaga tcgatcttat tgataaccgg
     1561 gatgatctcc aggtcggctt ctagagccat atacagattg gccagtgtct gcgcctcaat
     1621 tccctgggtt gcgtccacaa tcagtaacgc gccttcgcat gctgccaatg cccgagagac
     1681 ttcatagcca aagtccacat gccctggcgt gtcaatcaag tttagctcat agcttaggcc
     1741 atctctggct gtatattgca tgcgcaccgc cgaggccttg attgttaccc ctttctcgcg
     1801 ctccaagtcc atgctgtcga gcacttgctc cgtcatatcg cgctcagaga tagtgcccgt
     1861 cagttgcagc aagcggtcag ccagggtaga tttcccgtgg tctatatggg caataatgca
     1921 aaagttacgg attagctcgg acataggcaa acaataagta taactcaaac aacataggct
     1981 acgaaaaata cttcgaggtc tttttgggat atgacatggg ctattaagat gaacctggta
     2041 tacaaggctg agaggccctc ataccacata caaagatccc gctctccttt taaatatcgg
     2101 agacaggggt tcgtattact ctctatgaag aagcgtacaa ttagctaccg gagatacgtc
     2161 cgtcaaccca cttcaactac gtcacgagct ggctctcata ggtgcgcatg gcgttgaacg
     2221 cggcaatggc aacctcaagc ataggcaaat ctggttcgcg agttg
//
LOCUS       DBNHOH010000352         3863 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_596874, whole genome shotgun sequence.
ACCESSION   DBNHOH010000352 DBNHOH010000000
VERSION     DBNHOH010000352.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3863)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_596874"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1943)
                     /locus_tag="AC16V7_06325"
     CDS             complement(<1..1943)
                     /locus_tag="AC16V7_06325"
                     /inference="COORDINATES: protein motif:HMM:NF021570.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HIR6256770.1"
                     /translation="MLSALLWYFTITLIGWITFPLVYRLLPALVDRGYAFTRTVGLLL
                     WAYAFWLLASLGILRNNLGGLLFSVLLLIALSGWALRSVSLEEMADWLRKQRSLVLVV
                     EILFLVAFAFMGLVRAANPEILGTEKPMELAFINAILNSPEFPPHDPWLSGYAISYYY
                     FGYVMVAMLAKLTGTPGGVAFNLGVTLVFALSAVGSYGLVYNLLSFRSTRLPRRQMDS
                     SGRAAQAKSTFLSLALLGPIFVLLIGNLEGFLEVLHARGLFWQSTPTGELTSPFWKWL
                     DMRELSSPPQQPFSWTPSRYLWWWRASRVVQDYDFAENWKEVIDEFPAFSYLLADLHP
                     HVLAMPFAFLAMGLALNLFLGGGEGRLSWLQRGLGERVESWLSLEINPPVFLLGAVCL
                     GGLAFLNTWDFPFYVALFAGTFALVRMRQVGTGWGVGISEFFRLTIALVVTGIILYLP
                     FYIGFSSQAGGVLPNLVYPTRGTHLWVMFAPLLLPLFAFLYFRWMHSSGLGFWKGGAL
                     ALSLTLLLWLLSILLTVGIAMLPEVNNLFLGSLSAPDYGNLLAESLVRRIRSPGGWLT
                     LLILLAMTLGLMLKAVGEKKIEENVEGSRALSNRESAQDVPQSLSPNIFVLLLILLGT
                     LLVLGPEFLYLRDLFGWRINTIF"
     gene            complement(1945..>3863)
                     /locus_tag="AC16V7_06330"
     CDS             complement(1945..>3863)
                     /locus_tag="AC16V7_06330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HIR6256771.1"
                     /translation="LFSMIAMGAFSLGWNLFNSRYLFDNRQPFMQGSEPSFFNRISPF
                     IPALAAAFGMAVLGNLGTVRMLFQGYQRIVAPDGVIEGVGLLTRWGWAVRGFVRVLSG
                     ANLPYSIGDWYWIPSRAIPALNDVEPITEFPFFTVLYADPHAHLFALPIALLVLGIAL
                     SLVLGRARWKSRLEVVCGFVLAGLAIGALRPTNTWDFYPYLALGSVAIIYALGLYFRP
                     VAERPLPLIGRLPPSSQRLVVTISGFLLLLALSMFLYQPYATWYALGYSKLAIWKGSH
                     TTLSAYLTHWGLFLFVIVSWMVWETRDWLASTPVSALRKLKPYRELVLSSPFVLLGVV
                     LLLAFYLEVAIAWLVLPLAVWAGVLLLRPNQSDAKRFVLFLVGSGLLLTLMVEVIVLV
                     GDIGRMNTVFKFYLQVWTMFAVCAAAALGWILTDISGWLPGWRVSWQVVLTILGFGAA
                     MFSLMGSMAKVEDRMSLEAPYTLDGMAFMAHTQYEETWGTMDLSQDYRAIRWMQENVS
                     ASPVIVEANQLNLYRWGSRFSIYTGLPGVVGWEWHQKQQRAAIPTNWVSERIAEIETF
                     YTTTDLLQAQSFLSKYDVRYIILGQQERGLYAGEQGEQLEGLAKFPAADGLLWRSVYR
                     DGDTIIYEVMDKGN"
BASE COUNT         1106 a         1115 c          951 g          691 t
ORIGIN      
        1 ttaaatatgg tgttgatgcg ccacccaaac agatcgcgca aatagaggaa ttccggcccg
       61 aggacgagca gtgtccccag caggatcagc agcaagacaa aaatatttgg tgaaagtgac
      121 tgcggcacat cctgggcact ttctctattg ctcaacgccc tgctgccttc cacattctcc
      181 tcgatcttct tctctcctac cgccttgagc atcagcccca gggtcatcgc cagcaggatc
      241 agcagtgtga gccaaccgcc tgggctcctg atccggcgga ccagcgattc cgccagcaga
      301 ttgccataat cgggcgccga gaggctaccc agaaacagat tgttcacttc gggcaacatg
      361 gcaatcccca ccgtcagcaa gatggagaga agccacagta gtaatgtgag actcaatgcc
      421 agcgcaccac ccttccagaa accaagccca gaggagtgca tccaacgaaa atacaaaaag
      481 gcaaacaatg gcaacagcag tggggcaaac atgacccaaa ggtgcgtccc gcgcgtcgga
      541 tataccaggt ttggcagaac tcccccggcc tgagatgaaa agccgatgta gaatggcagg
      601 taaaggatga tccccgtaac gaccaatgcg atggttaagc ggaagaattc actgattcca
      661 actccccacc ctgtgcctac ctgtctcata cgcaccaaag cgaaagtccc agcgaacagg
      721 gctacataaa aggggaaatc ccaggtattc aagaatgcca atccacccag gcagactgca
      781 cccagaagaa atactggagg gttgatctcc agggataacc atgactctac gcgctcgccc
      841 aatccccgct gcaaccagct caggcgcccc tcccctccac ccaaaaatag gttgagcgcc
      901 agtcccatcg ccaggaaggc aaatggcata gccaaaacat gagggtgcaa gtctgccagc
      961 agataggaga aagctgggaa ttcgtcaatg acttctttcc aattttcagc gaaatcgtaa
     1021 tcttgaacca cgcgcgaagc acgccaccac cacaaatagc gggagggcgt ccaggagaaa
     1081 ggttgctgcg ggggcgagct cagctcacgc atgtcgagcc acttccaaaa aggcgaagtg
     1141 agctcaccag tgggtgtgct ctgccagaac agcccgcgcg catgcagcac ttccaggaag
     1201 ccttccaggt taccgatcag caagacaaat atcggaccta ataatgccaa agacaggaag
     1261 gtggatttag cctgggctgc gcgccctgac gaatccatct gccgacgggg caaccgggta
     1321 gatcgaaaag acagcaggtt atatactaat ccgtaagacc caacggcgct caaggcaaag
     1381 accagggtga cccccaggtt aaacgccacg ccgccaggtg tgccagttag cttagccagc
     1441 atggcgacca tgacatagcc gaagtaataa taagaaattg catacccaga aagccatggg
     1501 tcatgtggag gaaactcagg cgagttcaag atcgcattga tgaaggccag ttccatgggt
     1561 ttctcagtgc ccagaatctc aggatttgcc gcccgcacca accccataaa agcaaatgcc
     1621 accagaaata ggatttccac caccaatacc aaagatcgtt gcttgcgcag ccaatcggcc
     1681 atttcctcca gcgaaacact gcgcaatgcc catccgctca gggcaatgag gagcagcaca
     1741 gagaacaata gcccacccag attattgcgc aaaattccga gcgaagccag caaccaaaag
     1801 gcatacgccc aaagcaacaa tcccacagtg cgcgtgaagg cgtatcctcg atccaccagt
     1861 gctggaagca gccggtacac aagtgggaaa gtgatccagc cgatcagcgt gatagtgaaa
     1921 taccagagga gggcggaaag catattaatt tcctttatcc atgacctcgt aaattattgt
     1981 atctccatca cggtacacgc tgcgccacaa caatccatct gcggctggga actttgccaa
     2041 gccctccaat tgctcgccct gttcaccggc atacaaaccg cgctcttgtt gtcccagaat
     2101 aatgtaacgc acatcatact tgctcaagaa gctctgtgcc tgtaacaggt ctgttgtcgt
     2161 ataaaaagtc tcgatctcag caatacgctc gctgacccag tttgtcggta tggcggcgcg
     2221 ctgttgcttt tggtgccatt cccaccccac gacaccaggc aacccggtat aaatcgaaaa
     2281 gcgcgatccc caacgataaa ggttgagctg gttcgcttca acaatcactg gcgaggcgga
     2341 gacgttctcc tgcatccatc ggatggcgcg gtaatcttgg ctaaggtcca ttgtccccca
     2401 ggtttcttcg tattgtgtat gagccataaa ggccattcca tccagggtgt atggcgcttc
     2461 taagctcatc ctgtcctcaa ccttagccat gctgcccatc aaagagaaca tagcagctcc
     2521 aaaacccagg atcgtcaaga ccacctgcca ggaaacccgc catcccggta gccatccgga
     2581 tatatccgtt aatatccaac ccagtgcagc tgcagcgcag acggcaaaca tcgtccacac
     2641 ctgcaagtag aatttaaaca ctgtattcat gcgaccaatg tcgccgacca ggacaataac
     2701 ctctaccatt agagtcagca gcaggccgct tcccaccaag aagaggacaa agcgcttggc
     2761 atctgattgg ttggggcgca acagcaacac acccgcccag actgccaagg gcaataccag
     2821 ccaagcaatc gccacctcca aatagaaagc caacagcaga acaacaccca aaagcacaaa
     2881 gggtgacgaa agaaccaatt cccgatatgg tttgagcttg cgcaaggccg agacaggtgt
     2941 ggaagccagc caatcgcgcg tttcccaaac catccatgag acgatcacaa acaggaaaag
     3001 gccccaatgg gtgaggtaag cactcagggt ggtatggctg cctttccaaa tggctagctt
     3061 cgagtaaccc agcgcatacc aggtagcata tggttggtaa agaaacattg agagagccaa
     3121 gagcaacaag aagccactga tagtaaccac aagccgctgg gagctgggcg gcaatcgccc
     3181 gatcagcggt aatggtcttt cagcaaccgg gcggaaatat agcccaaggg catatattat
     3241 tgctacgctt cccagcgcta ggtaaggata aaaatcccag gtattggtag ggcgaagcgc
     3301 gccaatggct agacccgcca ggacgaaccc acacactact tccagtctgc ttttccagcg
     3361 ggcgcgccct aatacgagcg acaaggcgat ccccaatacc agcagggcga tgggcaaagc
     3421 aaagagatgt gcgtggggat cagcatatag cactgtaaag aacggaaact cggttatcgg
     3481 ctccacgtcg ttgagagccg gtatggcacg actgggtatc caataccagt cgccgattga
     3541 atagggcaag ttcgccccag agagcacgcg cacaaatccg cgcaccgccc acccccagcg
     3601 cgtcaatagg ccaacgcctt caatcacgcc atctggggcg acgatgcgct gatatccctg
     3661 gaaaagcatt ctcacagttc caagattccc tagcactgcc atcccaaaag cagctgccag
     3721 ggcagggatg aacggcgaga tacgattgaa aaatgacggc tcactgcctt gcataaatgg
     3781 ctggcgatta tcgaacagat accgagaatt gaacaaattc cagcccagcg aaaaggcgcc
     3841 catggcaatc atggaaaaca gcg
//
LOCUS       DBNHOH010000353         1451 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1302303, whole genome shotgun sequence.
ACCESSION   DBNHOH010000353 DBNHOH010000000
VERSION     DBNHOH010000353.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1302303"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /gene="lpdA"
                     /locus_tag="AC16V7_06335"
     CDS             <1..1221
                     /gene="lpdA"
                     /locus_tag="AC16V7_06335"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIR6256772.1"
                     /translation="HTLRERGKELGFSFENLTLDYSVAVKRSRQVSERLVKGVGFLMK
                     KNKIDVHMGAARVTASDTVAVTDKDGNIVELKTKNIVVATGASVAVPPGWEIDGEKIV
                     TYLEAILQEKLPKSVIIIGAGAIGVEFATVWNSYGVDVTIVEMLPRLVPLEDEEVSTE
                     LTRAFNKRKITSLVGHRVEAIAATKGGVQVKVSSEAGEKTLEAEQALVAIGFTPNGRG
                     LGLEEVGVKLSQRGAVEIDERMATNVPGIWAIGDVTAKLMLAHVGSAQGIICAENIAG
                     AETVTLDYEMMPRATYSQPQVASFGMTEAQAKERGYDVKVGRFPFQASGKALGMGESA
                     GWVKILTDAKYGEILGAHMVGPEVTELLPELTIAQMMELTTAEIARNVHAHPTLSEVL
                     MEAAHGAEGHAINI"
BASE COUNT          315 a          383 c          468 g          285 t
ORIGIN      
        1 cacactctgc gcgagcgcgg caaggagctg ggcttttcgt ttgagaatct caccctggat
       61 tacagcgtgg ctgtcaagcg cagccgccag gtctctgagc ggctggtcaa gggggtgggc
      121 tttctgatga agaaaaacaa gatcgatgtc cacatggggg ctgcccgggt gacagccagc
      181 gacacggttg ccgtgacgga taaagatggc aatatcgtgg agttgaagac caagaacatc
      241 gttgtggcaa ctggtgccag tgtggcggtc ccgccgggct gggagatcga cggcgagaag
      301 atcgtcacct atttggaagc cattttgcag gagaagttgc ccaaatcagt cattatcata
      361 ggcgccgggg cgatcggcgt ggagtttgcc acagtgtgga attcctacgg tgtggatgtg
      421 accatcgttg agatgctgcc gcggctggtg cccctggagg acgaggaggt cagcaccgag
      481 ctgacgagag ccttcaacaa acgcaagata acttcactgg tcgggcatcg cgtggaagcc
      541 atcgcagcca caaagggcgg cgtgcaggtc aaggtctcca gtgaggccgg cgagaaaacc
      601 ctggaagccg agcaggcgct ggtggctatc ggtttcacac ccaacgggcg cggcttgggc
      661 ttggaggaag tgggggtaaa actcagccag cgcggcgcag tggagatcga cgagcgcatg
      721 gcgaccaacg tgccaggcat ctgggccatc ggcgatgtaa ccgccaaatt gatgctagcc
      781 cacgttggat cggcgcaggg gatcatctgc gctgagaaca tagctggcgc tgagacagta
      841 accctggatt acgagatgat gccgcgcgct acctacagcc agccacaggt ggcctccttt
      901 ggcatgaccg aagcccaggc caaggagcgc ggttatgatg ttaaggtagg gcgcttcccc
      961 ttccaggcta gcggtaaggc gctcggcatg ggcgaatcag ctggctgggt gaaaattctc
     1021 accgatgcta aatacggtga gatcctgggc gcgcacatgg ttggtccgga ggtgactgag
     1081 ctgctccctg agctaaccat cgcccaaatg atggaactga cgacggctga gatcgcccgc
     1141 aacgtccacg ctcatcccac ccttagtgag gtgctgatgg aggcggctca tggagcagaa
     1201 gggcacgcca tcaatatcta agtcctatat ctggtatcca ttgctggaac attgaaattg
     1261 ggacaggcga ttcattttat ctttcgtttg aaaaaacccg ctgataatat ctatctgcct
     1321 gccccatcac ccatttatat tctgcccgct cagcgatcag gcgggtgttt tcctcccggg
     1381 cgcgtaggcg catctctggg tctcgtaggg cgcgcaggat cgcctccgac agcgcgctcg
     1441 gggagcgggg g
//
LOCUS       DBNHOH010000354         2636 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_84078, whole genome shotgun sequence.
ACCESSION   DBNHOH010000354 DBNHOH010000000
VERSION     DBNHOH010000354.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_84078"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..1451
                     /locus_tag="AC16V7_06340"
     CDS             177..1451
                     /locus_tag="AC16V7_06340"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR6256773.1"
                     /translation="MHLESQESPGSHLSLLYQLTQTFNSSLNLDEVLNRVMDEVIAAT
                     HAERGFVMLLEADEKLSFRIARGMDQTTIDAPQFQVSRSVVERVAQKGQAVLTSDAQT
                     DERFNIRQSVVLLGLRSILCVPLKLKGRVIGVIYVDNRLQAGIFTEADLRLLSAIASS
                     AAVAIENARLYQVAVEKGRLEQELSMARRVQVSLLPRKMPQLSGWDFITRWKPARQVG
                     GDYYDFIETDDGLLGLVIADVTDKGMPAALFMAFTRSIIRGNLDHATSPAAGINRVNR
                     LICQESDNGLFVTLFYGLLDPNTGQLTYVNAGHNPPILISSSELGHSTPMLYLESTGI
                     PLGIEEETSYTQNVVQLKPGDFITLYTDGITEAIDASEQEFGLERLQHVLLDHQDQSA
                     SEIAVALEEAIDHYSGDLTSHDDITFIIAKRL"
     gene            1460..>2636
                     /locus_tag="AC16V7_06345"
     CDS             1460..>2636
                     /locus_tag="AC16V7_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR6256774.1"
                     /translation="MTTLSQLMGRYQLMQELGKGGMGMVYEGHDNLLNRQVAIKVLSE
                     ARNIRLGSEGRSRLLHEAQAAAGLNHPNIVDIYDAGEDQGVSFIVMELVKGRSLHEHK
                     PKSIDEIISIACQICAALDHAHTHGIVHRDLKPENVLVTPDGTAKLTDFGLARSVASH
                     ISLDGMIVGTVYYLAPEAALRQSYDGRADLYSMGVMLYELTTGLLPFTADDPLAVISQ
                     HLYAPLVPPRAHNPQIPEALDALIVQLMSKQPEDRPRNAGEVWQLLELLETAQQLGMK
                     KPAGQEYTLLDRIVRGRLIGREREVAQMSAAWQQSTSGQGGVLLISGEPGIGKTRLAR
                     ELVAQALISGGKVMTGACYAEGGIPFDPFPQIIREAFDSSDSHVDLPRYFLADLAQIA
                     "
BASE COUNT          656 a          703 c          662 g          615 t
ORIGIN      
        1 accagcgaat atgtcacccc caccaaaacg ccccaaacac tctctttgcg gggttgcgca
       61 tgcccctcag cgcatatgca attgccctga agccaaacca tgccggatac gattccattc
      121 ataaaggggt ataatacccc aaatatacct cggaatatat tcaatagatc aggaaaatgc
      181 acctcgaatc ccaagaatca ccgggcagcc atctcagtct gctctaccag cttacgcaaa
      241 ccttcaattc ctcgctcaac ctggacgaag tgttaaaccg ggtgatggac gaggtcattg
      301 ccgccaccca tgcggagaga ggttttgtta tgctgctcga ggccgatgag aaactcagtt
      361 ttcgaattgc ccgtgggatg gatcaaacga ctattgacgc accccagttc caggtatccc
      421 gcagcgtagt ggaacgtgtt gcccaaaaag gacaagcggt gctgaccagc gatgcccaaa
      481 cggacgaacg ttttaacatc cggcagagcg tcgtgctcct gggtctgcgc tcgatcttgt
      541 gtgtcccttt gaaactaaaa ggccgcgtga tcggcgttat ctacgtggat aatcgtctcc
      601 aagcgggcat cttcactgaa gccgacctgc ggctgctcag cgcaatcgcc tccagtgcag
      661 cagtagccat tgagaacgct cgcctctacc aagtggcagt cgagaaaggg cgcttggagc
      721 aggaattaag catggcacgc cgtgttcagg tcagcttgct tcctcgcaag atgcctcagc
      781 tatccggctg ggattttatt actcgctgga aacccgctcg tcaggtaggt ggtgactatt
      841 acgactttat tgagaccgat gatggactgt taggcctagt gatagccgat gtgactgata
      901 aaggtatgcc tgccgcgctg ttcatggcct tcactcggag catcatccgt ggcaacctag
      961 accatgccac cagtcctgct gctggaatta accgtgttaa ccgcctgatt tgccaggagt
     1021 ctgataacgg gctttttgtc actctgtttt acggactgct cgatccaaat actgggcagc
     1081 tcacatacgt gaacgctgga cacaaccctc ccatcctgat atcatcctcg gaattgggac
     1141 actccactcc aatgctgtat cttgaaagca ctggcatacc gcttgggatt gaagaagaaa
     1201 cttcatatac acaaaacgtt gttcaactca aaccagggga ttttataacc ttatataccg
     1261 atgggattac cgaagcgatt gatgcgagtg agcaagagtt tggattggaa cgattgcaac
     1321 acgtgctctt ggatcaccag gatcaatctg cctccgagat cgctgtagcc ctagaagaag
     1381 ctattgatca ctactccggc gatttgacat cccatgacga tatcacattc atcatagcta
     1441 aacgcttata gaaaaaacga tgacgacact tagccaactt atgggtcgct atcagcttat
     1501 gcaagaactt ggcaaaggtg gcatgggtat ggtttatgag gggcacgata acctcctgaa
     1561 ccgccaggtc gcaatcaaag ttctcagcga ggcgagaaat attcgcttgg gtagcgaggg
     1621 tcgctcgcgg ttattacatg aagcacaggc agctgctggt ttaaaccacc ccaatattgt
     1681 cgatatctac gacgccggtg aagaccaggg ggttagcttt atcgtcatgg agttggttaa
     1741 gggaagatca ttgcacgagc ataaaccaaa gtcgatcgat gagatcatat ccattgcctg
     1801 ccagatatgt gccgcgctgg atcacgccca cactcacggg attgtccacc gcgatctcaa
     1861 gccagaaaac gtgctggtaa cccctgatgg gaccgctaag ctgactgact ttggactggc
     1921 gcgctctgtc gcctcacata tatccctaga tgggatgatt gtgggcacag tgtattacct
     1981 agcccctgag gcggctcttc gtcaatccta tgatggtcgc gcagatctct attcaatggg
     2041 agtgatgtta tacgagctca ctaccggatt gctgcccttt actgcagatg atccgctggc
     2101 tgtaatatct caacatttgt acgcgcctct tgtcccaccc cgcgcgcata atccccaaat
     2161 tccagaagcg ctggacgctc tcatcgtgca actaatgagc aaacaacccg aggaccgtcc
     2221 gagaaacgcc ggcgaggtat ggcagctatt ggaactgctt gagactgccc aacagctcgg
     2281 catgaaaaaa cctgctggcc aagagtacac tctactagat cggattgtgc gcgggcgctt
     2341 gatagggaga gagcgagagg tcgctcaaat gagtgctgcc tggcagcaat ctacatctgg
     2401 gcagggaggt gtattgctta tcagcggaga gcctggtatt ggcaaaaccc gcctggcacg
     2461 cgagctggtg gctcaagccc tgatctccgg tgggaaggtt atgaccggtg catgctatgc
     2521 agaaggaggt atccccttcg atccattccc tcagatcatt cgagaggcct ttgattcatc
     2581 cgattcccac gtagacctcc cacgttactt ccttgcagat ctagctcaaa ttgcac
//
LOCUS       DBNHOH010000355         2947 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_276436, whole genome shotgun sequence.
ACCESSION   DBNHOH010000355 DBNHOH010000000
VERSION     DBNHOH010000355.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_276436"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="AC16V7_06350"
     CDS             <1..501
                     /locus_tag="AC16V7_06350"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HIR6256775.1"
                     /translation="TTMLQENSPTLAETLDFLGELGVPTIGLNALIYSGHGLTVGTGL
                     RERDLQPLLELARQRTEQYAQRLIWYTPTQYCNFDPMQLELGVKGCTAALYNMCVEPD
                     GVVLPCQSYYQSLGNLLIDPWDKIWSHDLAVSLRERRGIPSKCNDCVLLAECGGGCPL
                     MFNLNN"
     gene            513..1661
                     /locus_tag="AC16V7_06355"
     CDS             513..1661
                     /locus_tag="AC16V7_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HIR6256776.1"
                     /translation="MTSLFAPIRRLFDPIKPIPQGIYHYQSPLEDPRNYRLHLRIEED
                     GSGIMIVNASTVLHLNQTAAEYAYYFVKNTPAEKVTQLVSQRYSVDPAQAAKDYHNLT
                     ERILTLIEVPDLDPVTFLDFERKTPYARTILAPYRLDCALTYRLPKGADPEAAPKKRV
                     DRELNTQEWISVLDKAWHAGIPHIVFTGGEPTLREDTLTLIAHAEHNGQVSGLLSDGL
                     RLVEEAYLVDLLQTGLDHLMMVLEPENDLSWQALGKVLAADLFTAIHLTLTPQNAASL
                     PGYIKKLAEMGTPAISLTSQDPSLGETLQALRDHAADLQMSLVWDLPVPYSSQNPIAM
                     ELVEEKLVDGAGRAWLYVEPDGDVLPAQGLNQILGYILNDPFEKIWNR"
     gene            complement(1658..2302)
                     /locus_tag="AC16V7_06360"
     CDS             complement(1658..2302)
                     /locus_tag="AC16V7_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012797.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR6256777.1"
                     /translation="MDTHGIQVRKCVRAECGMRFPLLLSDQHGKRCPECGASTRIVLE
                     KIVAPESGGELKTYHNIKIEAMLDNIRSAWNVGSMFRTADGVGIRYMHLCGLTPTPDN
                     PKVTKTSLGAEVSVPWLSYRDGVRTAGELKGKGMRLWALEHNPGAESMLELQDIPADT
                     SIVLVVGNEIAGVDPGILELCERVMYLPMIGLKRSYNAAVAFGIAAHYLRFAVP"
     gene            2330..>2947
                     /locus_tag="AC16V7_06365"
     CDS             2330..>2947
                     /locus_tag="AC16V7_06365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6256778.1"
                     /translation="MSANETPINWHLRFAQQARWTEDVRRFLFTRAAIESARRVLDVG
                     CGTGVLLDELEGNFDTALYGIDIASNYIKMAMRNTQRSFFTQSDAHALPYPSQSFDLA
                     YCHFLLLWVSEPWKVIQEMARVVRPGGAVLALAEPDYGGRIDYPSELEELGQAQHASL
                     AIQGADPLMGRKLSEIFHQAGLTSIETGVLGGNWTSSPSLEEWQDE"
BASE COUNT          705 a          823 c          714 g          705 t
ORIGIN      
        1 actactatgc tccaagagaa cagcccaacc ttggcggaga cgctcgattt tcttggtgag
       61 ctgggcgtcc ctaccattgg gctcaatgca ttgatctatt cgggtcatgg cctcacggtg
      121 ggaaccggat tgagagaaag agatttgcaa ccgctcctcg aactggcacg ccagcgcacc
      181 gaacaatatg cccaacgctt gatctggtac acacccaccc aatattgcaa tttcgacccg
      241 atgcagcttg aactgggcgt caaagggtgc actgcagcgc tctacaacat gtgcgtcgag
      301 ccagatggtg tggtattgcc ttgccaatct tattatcagt ccctgggtaa tttactgatt
      361 gatccgtggg acaagatttg gtctcatgat ttagcggtgt cattgcgcga gcgccgcggc
      421 atcccgtcaa aatgcaatga ctgcgtcctt cttgctgagt gcggaggcgg ttgcccgctt
      481 atgttcaacc tgaataattg aggaaaaaag ccatgacatc cttattcgcc ccaattcgcc
      541 gcctgttcga cccgatcaaa ccgatacccc aaggtattta ccattaccaa tcaccgctcg
      601 aagacccgcg gaattatcgc ctacacttgc gcatcgaaga agatggcagc ggcattatga
      661 tcgttaacgc ctccaccgtg ttgcacctca accaaactgc ggctgaatac gcttactatt
      721 tcgtcaagaa cacgcccgcc gagaaagtta cccaactggt ctcacagcgt tacagtgttg
      781 atcctgcgca agctgctaaa gactaccaca acctcaccga acggatcctg accctgatag
      841 aggtacctga cctcgacccg gtcacattcc tggatttcga gcggaagaca ccctacgcac
      901 gcacgatttt ggctccctac cggctggact gcgcccttac ataccgccta ccaaagggcg
      961 ctgaccccga ggctgctcct aaaaagcgcg tagatcgaga actgaacacg caggaatgga
     1021 tttcagtcct ggataaagcc tggcatgcgg gcattccaca tatcgtattt actggcggtg
     1081 agcccacttt gcgagaagac actctgacat tgattgccca tgcggagcat aatgggcagg
     1141 ttagcggctt gctatccgat ggtctgcgcc ttgttgaaga ggcttacttg gtcgatctgc
     1201 tccagactgg tttagaccac ctgatgatgg tcctggaacc cgagaatgat ctttcctggc
     1261 aagctttggg gaaggttctc gcggccgatc tctttacagc aatccacctg acactcacac
     1321 ctcaaaatgc cgcatctctt cctggataca taaagaagct tgccgagatg ggcacccctg
     1381 caatctccct cacttctcaa gatccaagcc tgggagagac gcttcaagcg ttgcgggatc
     1441 acgctgctga tttgcaaatg tcgcttgtat gggatttacc agtgccttac tcaagccaga
     1501 atccaattgc tatggagctg gttgaagaga aactggtgga tggcgctgga cgcgcttggc
     1561 tttacgttga gcctgatggc gatgtgctcc ccgctcaagg cctcaaccaa atcctgggct
     1621 acatactaaa cgaccccttc gagaaaatct ggaatcgcta aggcacagca aagcgcagat
     1681 aatgcgctgc aattccaaaa gccactgccg cgttatagga acgctttaaa cctatcatcg
     1741 ggagatacat cacccgctca caaagctcca agatcccagg gtccactccc gcaatttcgt
     1801 tccccactac cagcacaatc gaggtgtctg ctggaatgtc ttgcagctca agcatcgatt
     1861 ccgcacctgg attatgttcg agagcccaga ggcgcatgcc cttcccttta agctcgccgg
     1921 ctgtgcgcac gccatctcta taggataacc agggtacgct tacttctgca cccagtgaag
     1981 tcttcgttac cttaggatta tccggcgtcg gggtcaatcc acacagatgc atataccgga
     2041 tgccaacccc atctgccgtg cggaacattg agccgacatt ccatgcactg cggatattat
     2101 ccagcatcgc ctcaattttt atgttgtggt aagtttttaa ttccccacca gattcgggcg
     2161 cgactatttt ttccagcacg atgcgcgtcg atgccccaca ttcgggacag cgcttcccat
     2221 gttgatcgct taggagcagg ggaaagcgca tcccgcattc agctcgaaca cacttacgca
     2281 cctgaattcc atgggtatcc ataggcacat tccattataa tcactttata tgtctgcaaa
     2341 tgaaacacca ataaattggc acctgcgttt tgcacagcag gcacgctgga cagaggatgt
     2401 ccgccgtttc ctgttcacac gggcggctat cgagagtgct cgtcgtgtcc tggacgtggg
     2461 ctgcggcaca ggtgtgcttt tggatgagtt ggaagggaac ttcgatacgg cattatatgg
     2521 cattgatatc gcatcaaact acatcaagat ggctatgcgc aatacgcaaa gatcattttt
     2581 tactcaaagt gatgctcacg cgcttcccta cccttctcaa tcatttgatc tggcatactg
     2641 ccattttttg cttttatggg tcagtgaacc ctggaaagta attcaagaga tggcccgtgt
     2701 agtacgacct ggcggcgcag tgttagcctt agccgagcca gactatggcg ggcgcatcga
     2761 ttacccatcc gagttggaag aactgggaca ggcacaacac gcttccttag caattcaggg
     2821 cgctgatccc cttatgggtc gcaagctgag cgagattttt catcaagctg gcctcacatc
     2881 catcgagact ggcgtgctcg gtggaaattg gacttcttcg ccttcgctag aagaatggca
     2941 agacgaa
//
LOCUS       DBNHOH010000356         1288 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1558494, whole genome shotgun sequence.
ACCESSION   DBNHOH010000356 DBNHOH010000000
VERSION     DBNHOH010000356.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1558494"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="AC16V7_06370"
     CDS             <1..315
                     /locus_tag="AC16V7_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256779.1"
                     /translation="RAEILIGRGLYPRYFLAGQGEAPESRWPSFVVRDYNRLGFLLIG
                     PEQRQVVLRTPVSPPYFPHASDVLVLGCSEGDYLDAFMVVFLESPNKTLVRSPLEGWA
                     CP"
     gene            306..>1288
                     /locus_tag="AC16V7_06375"
     CDS             306..>1288
                     /locus_tag="AC16V7_06375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR6256780.1"
                     /translation="MSIDRSPSWHKYLHLVPPLVFFMLMFLYFPFRERFEFDPDEGYE
                     AMKAFLVVQGYPLYTEIWSDQPPLFTYLLAPVIRVFGPDIDAARTLVLLFSTALMLAC
                     SIFLCSNWGVPHALASSILIFLLPFYNTLSVSVMVAVPILAFALLSLIALSMWHKGHK
                     DRWLIFSALALALSIITKLFIAFLAPIFALGILFDMRSRMGRNANWLQVLRPATLWCL
                     TLAIVLGGLGLLLVGPANLGQLLNTHLAASQSGTYIARANATPIIWHLGDAWPILLLA
                     IPGCLFVLLERRWISLYLLAWLASAYLLLSVIVPVWYHYQLLVTIPAAMLAA"
BASE COUNT          211 a          400 c          315 g          362 t
ORIGIN      
        1 cgtgcggaga tcttgattgg tcgtgggcta tatcctcgtt attttctcgc aggacagggc
       61 gaagcgcctg aaagccgttg gcccagcttc gttgtccggg attacaatcg cttgggcttt
      121 ctcttgatcg gccccgagca acggcaggtg gtattacgca ctccagtttc accaccttat
      181 tttcctcacg cttcagatgt gcttgtgctg ggctgctctg agggagatta cctagacgcc
      241 tttatggttg tgttcctgga atctccgaac aagacccttg tgcgctcacc gctggaagga
      301 tgggcatgtc catagatcgc agcccaagct ggcacaaata cctgcacctg gttccgccct
      361 tggtcttctt catgttgatg ttcctgtatt tccccttccg cgagcgtttc gaattcgatc
      421 cggatgaggg ttatgaggcc atgaaggcct ttctggtcgt ccagggttac ccgctctata
      481 ctgaaatctg gtccgaccag cccccgctct ttacttacct gttggctccc gttattcgcg
      541 ttttcggccc agatatcgac gctgcccgta ccctggtact gctcttctcc accgccttga
      601 tgctggcttg ctcaatcttc ttatgctcca attggggcgt tccgcacgcc ctcgccagca
      661 gcatcttgat attcctccta cccttctata acacgctcag cgtatcggtc atggtagctg
      721 tgccaatcct ggcctttgct ctgctctcct tgatcgccct ctccatgtgg cataagggtc
      781 acaaggatcg ctggcttata ttctccgccc ttgccctagc cctatccatc atcaccaagc
      841 tgttcattgc tttccttgcc cctattttcg ccctgggcat cttattcgat atgagaagta
      901 gaatgggcag aaatgcaaac tggctgcaag tgctccgccc ggctacactc tggtgcctga
      961 ccttggcgat tgttttaggc gggctgggcc tactactggt tggcccggcc aacctggggc
     1021 agctcctgaa cacccacctg gctgccagcc aaagcgggac gtatattgcg cgcgccaacg
     1081 ctaccccgat catctggcat ttgggcgatg cctggccgat tctcctgctg gccattccag
     1141 gatgtctctt cgtcctgctc gaacgccgct ggatttctct atacttgctt gcctggttgg
     1201 cttcagccta cctgctgctc tctgtaatcg tcccggtgtg gtatcactat caactgctcg
     1261 tcaccatacc ggccgccatg ctggcagc
//
LOCUS       DBNHOH010000357         3391 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1494330, whole genome shotgun sequence.
ACCESSION   DBNHOH010000357 DBNHOH010000000
VERSION     DBNHOH010000357.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1494330"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..170)
                     /locus_tag="AC16V7_06380"
     CDS             complement(<1..170)
                     /locus_tag="AC16V7_06380"
                     /inference="COORDINATES: protein motif:HMM:NF019544.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase-like protein"
                     /protein_id="HIR6256781.1"
                     /translation="MPRRYLDRPVWDEVVGLATEAGPVGQRLISGMHQSIAALSRAGV
                     NVVADHVLVEQE"
     gene            complement(249..1067)
                     /locus_tag="AC16V7_06385"
     CDS             complement(249..1067)
                     /locus_tag="AC16V7_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6256782.1"
                     /translation="MMDNYICITCGHQFAESKGPPERCPICEDDRQFVHPRGQSWTTL
                     DKLAREHRNVLVSLEKGLTSINTEPKFAIGQRSLLVQTSRGNVLWDCVSLIDEPTIEA
                     VGHLGGISALAMSHPHLAGSMVEWSRAFGDAPIYLHADLEPWIQRPDPVIQFWEGEDL
                     LLEEGLSLHRCGGHFEGSTVLLWPEGADGQGVLLTGDTMYVTPDRKHVSFMYSFPNFI
                     PLPAPVVERIVERALTLRFDRMYGHFADLDIMADAKQVVKRSAERYKRAIGVED"
     gene            complement(1166..1672)
                     /locus_tag="AC16V7_06390"
     CDS             complement(1166..1672)
                     /locus_tag="AC16V7_06390"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR6256783.1"
                     /translation="MSEVTEINGINVAELLEFKNICKQDPSKADRDPVLVAHWVGGSR
                     SRVEFKDKVIHLGGDDEFNPMQMFLATLAACDVDLVAMHASFLGIKIESLSVEVTGHF
                     NVRSYLGLEGAPGPGYDGISYTVRLSAPKITPEQVAQLRERCERSSPVGDSMARVIPL
                     KLEFTANT"
     gene            complement(1971..3230)
                     /locus_tag="AC16V7_06395"
     CDS             complement(1971..3230)
                     /locus_tag="AC16V7_06395"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011771208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HIR6256784.1"
                     /translation="MIMNEIGKKGRTAAHSVAHASQEQKRTVLLRLADQLVEASTEVL
                     SENQIDLQAARGAGLSDAMLDRLMLNEGRIHGIAADLRAVADLPDPVGEVFDEQTLSN
                     GLRVHKQRIPLGVLGVIYEARPNVTMDVAGLASKTGNSAILRGGSETINSNRILVSVI
                     QRALLENGLPPEVIQFVDDPDRARVYELLQLHEYVDMIIPRGGAALHDFCRENSRIPV
                     ITGGIGICHLFVDESADLEAGLQVIRNAKIQRPTVCNALDTALVHRNVAEQFLPKLVA
                     YLLQDGVSFRAHDSALPYLDQTTHESVKSAGPGDFDIEWLSLVLGLKVVNGLDEAIAH
                     IDEHSTGHSDGILTRDDENARRFVEQVDSAAVYVNASTRFTDGGQLGLGAEVAVSTQR
                     LHARGPMGLRELTTYKWVIIGDNHIRM"
     gene            complement(3227..3388)
                     /locus_tag="AC16V7_06400"
     CDS             complement(3227..3388)
                     /locus_tag="AC16V7_06400"
                     /inference="COORDINATES: protein motif:HMM:NF013626.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PUA domain-containing protein"
                     /protein_id="HIR6256785.1"
                     /translation="MLDQQGREIARGLVNYSAADLARVCGRHSSEIEKILGYMYGEEV
                     IHRNNMVLL"
BASE COUNT          831 a          986 c          800 g          774 t
ORIGIN      
        1 cactcctgct ccaccaacac gtggtctgcc accacattaa cgccggctct ggataatgcc
       61 gcaatggact ggtgcatgcc ggatatcaat ctctgcccca ccggacctgc ttccgtcgcc
      121 aaacccacta cctcatccca caccgggcgg tcaagataac gccggggcat gggcctacct
      181 cgtacatgaa accgcgctga gccttcatcc gctccagcga gcggctaggc agcatcgggg
      241 gtacagtctc aatcctcgac cccaattgct cgtttgtatc gctccgccga tcttttcacg
      301 acttgcttgg cgtccgccat gatgtctagg tcggcgaagt ggccgtacat ccggtcgaat
      361 ctcaaggtca aagcccgctc aacgatcctt tcaacaaccg gtgccgggag aggaatgaaa
      421 ttcgggaaac tgtacatgaa cgaaacgtgc ttgcgatcgg gggtcacata cattgtgtca
      481 ccggtcaaca acacaccttg gccatctgcc ccttcaggcc aaagcaggac cgtcgagccc
      541 tcgaaatggc cgccacatcg atggagcgag agcccttctt ccagtagcaa atcctcgccc
      601 tcccagaatt ggatgacggg atccggtcgc tgaatccaag gctcaaggtc ggcatgcaag
      661 taaatgggag catcgccgaa tgcacgactc cactcgacca tgcttcccgc caagtgggga
      721 tgggacatcg ccagagctga aatcccgccc agatggccta cagcctcaat agtgggctca
      781 tcaataaggc tcacacaatc ccacaggaca ttacctctgg atgtctgcac aagcaaagag
      841 cgctggccga tcgcgaactt cggctccgta ttaatgctgg tcaggccctt ctctagagac
      901 accaatacat tgcgatgctc tcgggcaagt ttatctaacg tagtccagga ctgaccgcga
      961 ggatgtacga actgccgatc atcttcacag attggacagc gctcgggggg tcccttgctc
     1021 tcggcgaatt gatgcccgca tgtgatgcag atgtagttgt ccatcatgtt ctactctcga
     1081 tcgcaatgct ggctgtttga gacatactta caaccgacag attcagatcg acaaaggaac
     1141 agacccttac caatcaaatg ccttgtcaag tattcgctgt aaattctaat tttaaaggta
     1201 tcacccgcgc catcgagtca cccacaggtg acgaacgttc acaccgctct cgtaattgag
     1261 caacttgttc aggggttatt ttgggagcag aaagacggac ggtgtatgaa ataccatcat
     1321 atccagggcc tggcgcacct tctaaaccta agtatgaccg tacattaaaa tgaccagtca
     1381 cttcaaccga gagactctcg atttttatac cgagaaagga agcgtgcatt gcaaccaaat
     1441 caacatcaca agcggcaagt gttgcaagaa acatttgcat tgggttgaat tcatcgtcgc
     1501 cgccgaggtg gataacttta tctttaaatt ccactcgcga acgtgagccg ccaacccaat
     1561 gcgcaactaa tacaggatcg cggtctgcct tgctgggatc ttgcttgcat atattcttga
     1621 actccagcaa ttcagcaacg tttatgccat tgatttcagt tacttcgctc attttttctc
     1681 ctttctatca aacatgcttt gacttgataa tactattggc ggaagcaggt tgcccaaaaa
     1741 gtgtatataa cgcaactatt cggttaattc ggatataaat catcctaata ggaattatac
     1801 accctaaacg ccacacttcc accgaaaaaa gcagggcaat tgcatatgcc ctgaggggca
     1861 tgcgcaatcc cgcaaaagag acctcaacca ggtccgcgac cggttgagct tgaagagcta
     1921 tgcctagttg tagtattagc ctgtcaaaag gagctacgtg cgaatcgagt tcacatgcgg
     1981 atgtgattgt caccaataat aacccattta taggtggtca attcgcgtaa acccatcgga
     2041 cctcgagcgt gcaagcgttg cgtggataca gccacctcag cgcccaatcc cagctgccca
     2101 ccgtccgtga aacgagtact tgcatttaca tacaccgcag ccgaatcgac ctgttccaca
     2161 aaccggcgcg cgttttcatc atctcttgtc agaataccat ccgagtggcc agtgctgtgc
     2221 tcgtcaatgt gagcaatagc ttcatcaagg ccattaacca ctttaagccc taaaacaagc
     2281 gagagccatt cgatgtcgaa atctccagga ccagccgatt ttacgctctc atgtgtggtt
     2341 tgatccaggt aaggcaatgc cgagtcatgc gcccgaaagg aaacaccgtc ttgcaacaga
     2401 taggcaacca gcttgggcag gaattgttcg gcgacatttc gatgcaccaa ggccgtatcc
     2461 agcgcgttgc atactgttgg tctctgtatc ttggcattcc ggatcacttg taaaccggct
     2521 tctaaatcag cgctctcatc tacgaacaag tgacagatac cgataccccc agtgataacc
     2581 gggatgcggc tgttctcccg gcaaaaatcg tgcaacgcgg ccccgccgcg tgggatgatc
     2641 atatccacgt actcgtggag ttgcagcaat tcgtacacgc gcgcccggtc tggatcatcc
     2701 acaaactgaa tcacttcagg cggcaagcca ttttccaaca aagcgcgctg aatcactgat
     2761 accaggatgc gattagagtt aatcgtctcg ctgccgccgc gcagtattgc cgaattaccg
     2821 gtcttgctcg ccaatccagc tacatccatg gtcacattcg ggcgggcttc atagatgacc
     2881 cccaatacgc ccagtgggat gcgttgtttg tggactcgca gcccgttgga gagcgtctgc
     2941 tcgtcaaata cctcgcctac cggatcgggc aggtctgcca ccgcccgtag atctgcggcg
     3001 ataccgtgta tgcgcccttc gtttaacatc agccgatcca gcatcgcatc gctcaagccg
     3061 gccccacggg ctgcctgcag gtctatctgg ttttctgaca agacctccgt cgaggcttcc
     3121 accaattggt cagccaggcg gagcaagacc gtcctctttt gctcttgcga ggcgtgagcc
     3181 actgaatggg cggcagtgcg ccccttcttt ccgatttcat tcatgatcat aacagcacca
     3241 tattattccg atggatgact tcttcaccat acatataacc caggatcttc tcaatctcgc
     3301 tggagtgtcg cccgcagacg cgcgcaaggt cagccgccga gtaattgacc aggccacggg
     3361 cgatctcccg cccttgctga tccagcacac g
//
LOCUS       DBNHOH010000358         2118 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_661727, whole genome shotgun sequence.
ACCESSION   DBNHOH010000358 DBNHOH010000000
VERSION     DBNHOH010000358.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_661727"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1174)
                     /locus_tag="AC16V7_06405"
     CDS             complement(<1..1174)
                     /locus_tag="AC16V7_06405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR6256786.1"
                     /translation="MAILLELIDALKNAQIEARFDLPTRILYSTDASIYQIAPLGVAF
                     PKSLDELAGTVELAASFDIPVLARGSGSSLGGQAIGHALILDCSRYLTRILEINPEDR
                     SAVVEPGVILSSLNKAAAEFGLQFGPDPASAERATMGGSLANNATGAHSILYGMAADH
                     IVSADVVLADGSITTFAEVTLEQARRIAGEVEEGLGDGGGHTSLSPISSPLVRSIYGA
                     SLRIREQYSGAIQARWPETWRRAAGYNLNYLLPWTPTSPPGWDEQFAYDRLSALSYPP
                     VSKGSINLAQLFAGSEGTPAVLRKASVRLVPLPKQTILGVLAFPDIAEACDAVLGLLE
                     LEPSAVELIPGILVNLARTVPAYASQLSIFDQLNHGSESPDLLAVEFSGDDPARLRE"
     gene            1526..>2118
                     /locus_tag="AC16V7_06410"
     CDS             1526..>2118
                     /locus_tag="AC16V7_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR6256787.1"
                     /translation="MSISQLARTIAESPTLALNEEARILRERGEPVINMSIGEPKNKA
                     PITAILSSAAKLTSGDVKYTPPDGTPSLKKAIIRYMEENYDRMVAPENVVISAGAKQS
                     LYNILFSILNPQDEVIILAPYWVSYPEMVKMCYGVPVFVTPEDGSFHPRMEEIERAVS
                     SYTKAIIVNSPNNPSGCVFSKEFIAQIVEFCERKNIYL"
BASE COUNT          554 a          549 c          530 g          485 t
ORIGIN      
        1 gctcccgcaa gcgagcagga tcatcaccgg aaaactctac agctagcaaa tccggagatt
       61 cactgccatg atttagctga tcgaatatag acagctgcga ggcatatgct gggacggtgc
      121 gcgccaggtt gaccaaaata cccgggatga gctcaactgc cgagggctca agctccagca
      181 atcccaaaac agcatcacaa gcctcagcaa tgtccggaaa agcaagcaca ccaaggatgg
      241 tttgtttggg aagcggaacc aaccgcaccg aagcttttct tagcacagct ggtgtgccct
      301 ctgagccggc aaataattgt gcgaggttta tagatccttt agatacaggc ggatatgaga
      361 gagctgacaa gcgatcatat gcaaattgtt catcccaacc cgggggtgaa gtgggcgtcc
      421 aaggaagcag gtaattgagg ttatatccag cggctctccg ccatgtctcg ggccatctgg
      481 cttgaattgc cccggagtat tgctcgcgga tacgcaaaga tgctccataa atagatctta
      541 ccaaggggga ggagataggg gatagagagg tgtgccctcc cccatcccct aatccttctt
      601 ccacttcccc tgcgatccta cgagcctgct cgagggtgac ttctgcgaag gttgttattg
      661 atccatctgc tagcacgaca tccgcagaga cgatatggtc ggctgccatg ccatacaaaa
      721 tcgagtgcgc gccagtggca ttgttcgcca ggctaccacc catggtagcc cgctcagccg
      781 aagctgggtc aggcccaaat tgcaagccaa attctgctgc agccttgttc aggctgctca
      841 gaatgacgcc tggctcaacc acagccgagc ggtcctctgg gttaatttcc agtatgcgcg
      901 tcaagtaacg cgagcaatcc agtatgagcg catggccgat cgcctgtccc cctaagctgg
      961 agccagaccc gcgggctaag acaggtatat caaaggaagc cgcaagctct acagtcccag
     1021 ctaattcgtc caaggattta gggaaggcta ctcccagggg ggcgatctgg tagattgaag
     1081 cgtccgtgct gtataggata cgtgttggaa gatcaaagcg cgcttcaatc tgggcgtttt
     1141 ttagggcatc aatgagctca agcaggatag ccatatttcg tcattttaga gcaaaaacca
     1201 taaacaagca agcgcaactc gttctactta gggcaatcgc atttgggcga tttcccacca
     1261 ctgaggagag aaaatggggt gttttggtgg ggcatacccc caccaaaaca ccccaaacac
     1321 tctttttacg ggcttgcgca tgcccctcag ggcatatgca attaccctgt cgcggtactt
     1381 gattaatgtc acattgacgc ctaatttgaa tagagataca ctctacattg atgcataatt
     1441 tattgcctta tcgaaaaatt catatttctc accctggagt atcgatttct ctcgatagaa
     1501 tctcattttg gagataaaag tgcccatgtc aatcagccaa ttagcgcgga caatcgccga
     1561 gtcgcccacg ctggcgctca atgaggaggc gagaatctta cgggagcgcg gggagcctgt
     1621 cattaatatg agcatcggcg aaccgaaaaa taaagcccca ataaccgcca tcctaagctc
     1681 agcagccaaa ctgacctcgg gcgatgtcaa atacacacct cccgatggga caccctccct
     1741 gaaaaaagcc atcatccgct atatggaaga aaattatgac cgcatggttg cgccggagaa
     1801 tgtcgtcatc tcggctggcg ctaaacaatc gctgtacaat attctcttta gtatcttaaa
     1861 tcctcaggat gaagtaatta tcctggcccc ctattgggtc agctatcccg agatggtcaa
     1921 gatgtgctat ggcgtgccgg tcttcgtcac gcccgaggat ggtagttttc acccgcgtat
     1981 ggaggagatc gagcgcgctg ttagctcata tacgaaagca atcatagtta atagccccaa
     2041 taatccttct gggtgtgtgt tttccaagga gttcattgct cagatcgtcg aattttgcga
     2101 gcgaaagaat atatatct
//
LOCUS       DBNHOH010000359         1608 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_982394, whole genome shotgun sequence.
ACCESSION   DBNHOH010000359 DBNHOH010000000
VERSION     DBNHOH010000359.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_982394"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /gene="queA"
                     /locus_tag="AC16V7_06415"
     CDS             <1..869
                     /gene="queA"
                     /locus_tag="AC16V7_06415"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HIR6256788.1"
                     /translation="GDVLVLNETRVIPARLFAKKLPTGGSVELLLLRRVDDHTWETLV
                     GGKGLVAGKRLQVVDGPLAEITASLEGAQRIVRFSEPIEGYLGQAGHVPLPPYIHEKL
                     TNPERYQTVYARAPGSAAAPTAGLHFTPRLIERLKNQGIHIAQVTLHVGLDTFSPVTE
                     NDPQEHVIHTEWCQVSTQAAEAINRAKREGGRVVAVGTTSVRTLESAASRLQTGDIVS
                     PFEGLTSLFILPGYRFRVVDAMITNFHLPRSTLIMLVSAFAGRERILEAYETAKREGY
                     RFYSFGDGMLIV"
     gene            919..>1608
                     /locus_tag="AC16V7_06420"
     CDS             919..>1608
                     /locus_tag="AC16V7_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011434002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR6256789.1"
                     /translation="MTDKRDDRVWRALNEEIVQCTLCPRLVAWREQVARERRRAYRDF
                     EYWGKPVPGFGDVGARVLVVGLAPGAHGANRTGRMFTGDGSGDFLYPALHKAGFCNQP
                     VSIHRDDGLSLRDIFISSVCRCAPPANKPTTQEIGNCLGYLEREMSMLENLQGIVALG
                     KIAFDYSLRLLRARYNDIPRLQFAHNAFYPLGQGIPWLLASYHPSRQNTQTGRLTEAM
                     FNQIWQSVEHAI"
BASE COUNT          361 a          401 c          477 g          369 t
ORIGIN      
        1 caggggatgt gctggtgctt aacgagacgc gtgttatccc agcgcgcctc ttcgcaaaaa
       61 agttgccgac aggtgggagt gtggagctgc ttctcctgcg tcgcgtggat gatcatacct
      121 gggaaaccct ggtgggaggc aaaggactgg tagctggcaa gcgattgcaa gtggtagatg
      181 ggcctctcgc tgagatcacc gcctcactgg aaggcgcgca gcggatagtg cgcttcagtg
      241 agcccattga aggttattta gggcaggctg gacacgtacc cttgcctcca tatatccacg
      301 agaagctgac caaccctgag cgttaccaga ctgtgtacgc ccgtgcaccg ggttcggcgg
      361 cagcgcccac agctgggctg cacttcactc ccaggcttat cgagcgcttg aaaaatcaag
      421 gcatacacat tgcgcaggta acactgcacg tcgggctgga tacattttct ccggtaaccg
      481 aaaacgatcc ccaggagcac gttatccata ccgaatggtg ccaggtttct acgcaggctg
      541 cagaagcgat taaccgggca aaacgagaag ggggaagagt agttgccgta gggaccacga
      601 gtgtgcgcac attggagagt gctgcatcgc ggctccaaac aggagatatt gtgagtccgt
      661 ttgagggtct caccagcttg tttattttgc ctggatacag gtttcgtgtg gtagatgcga
      721 tgattacaaa tttccatttg ccgcgctcga cattgattat gctggttagc gccttcgcgg
      781 gccgtgaacg cattttggaa gcctatgaaa cggcaaagcg ggaggggtat cgcttctatt
      841 cgtttggcga cggaatgttg atagtgtgaa ttgacacgcg caattgacca aagagggacg
      901 cgagaacagg atgcttcgat gaccgataag cgagatgacc gagtttggcg ggcgctgaac
      961 gaagagatcg ttcagtgcac gctctgcccg cggctggtgg cgtggaggga acaggttgcc
     1021 cgagagcgca ggcgcgccta ccgagacttt gagtactggg gaaagcctgt gcctggattc
     1081 ggcgacgttg gagcaagggt gctggtggtc ggcttagccc cgggagccca tggggccaac
     1141 cgcactgggc gtatgttcac tggtgatggc tcaggagatt ttctttaccc tgccctacac
     1201 aaagctgggt tttgcaatca gcctgtatct attcatcgag acgatgggtt gtcattacgc
     1261 gatattttca tttcatcggt atgccgctgt gctcccccgg ctaacaaacc tacgacacaa
     1321 gagatcggca attgcttggg ctatttagaa cgggaaatgt ccatgttgga aaacctgcaa
     1381 gggattgtcg cgttgggtaa aattgccttt gattattccc tgcgccttct gcgcgctcgc
     1441 tataacgata tcccgcgctt acagttcgcc cacaacgcat tctatccatt gggccagggg
     1501 ataccctggt tattggcttc ttaccatccg agccgccaaa atacacagac cggcaggctg
     1561 acagaggcga tgtttaacca gatctggcaa tcagttgaac atgctatc
//
LOCUS       DBNHOH010000360         1287 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1302849, whole genome shotgun sequence.
ACCESSION   DBNHOH010000360 DBNHOH010000000
VERSION     DBNHOH010000360.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1302849"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..>1287
                     /locus_tag="AC16V7_06425"
     CDS             95..>1287
                     /locus_tag="AC16V7_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MerC domain-containing protein"
                     /protein_id="HIR6256790.1"
                     /translation="MRKIPILHPFLFTAYPVVAFLAHNIEQTEINSALRPLLVGLASA
                     AILYILLKAVYRDNHRAGLLTSFLLILFFTYGHVYNAIEGFSILGMNIGRHRLLAPLW
                     MALAGFSFWRGFQSRRDLQPLSNRANIIALIALTMPLGQISAYQISLRSVRIGSANSK
                     VFSSAQIDPNRPLPDIYYLILDSYSRDDMLKKYYDLDNTPFIDQIEQMGFYVARCAQS
                     NYGQSELSMASSLNYDYLDNLGRQFNPENRRRTGLADLIQHSAVRSLLEDLGYTVVAT
                     DTGYDATRLSDADIYLAPRVSAEINDFEETFLRTTAARLFIEGAAILKLPPDWEKRDQ
                     AHRERILFQLEQLRQIPAIPGPKFVFAHIITPHWPYIFGPNGEPVHEHPDSKTGYRDQ
                     VIFINR"
BASE COUNT          316 a          356 c          288 g          327 t
ORIGIN      
        1 ttgagcaggt cttcgcatcg cgttacacca tccatcgggt tgaggccatc caagattcgc
       61 aacgctccct ctacctgatg gaggcacgca agtaatgcgc aaaatcccca ttttgcaccc
      121 ctttctattc accgcctacc cggttgtggc tttcctggcg cataatattg agcaaaccga
      181 gattaactca gcgctgcgcc cgttgctggt agggttggca agtgccgcaa tcctttatat
      241 cttgttgaaa gccgtataca gggataatca tcgagcaggc ttgctcacct catttttgct
      301 gatcttattc ttcacctatg ggcatgtcta taacgctatt gaaggtttct caatcctagg
      361 gatgaacatc ggtcgccatc gcttgctggc gccgctctgg atggcattgg ctggtttttc
      421 cttttggcgg ggatttcaat caaggcgtga tctgcagcca ttaagtaatc gagccaatat
      481 catcgcctta attgcgctca ccatgccgct gggacagatc agtgcatacc aaatcagcct
      541 tcgatcggtc agaattggct cagcaaattc aaaagtgttt tccagtgccc aaatcgaccc
      601 aaaccgccct cttccagata tttattatct aattctggac agttattcgc gcgatgatat
      661 gctcaagaaa tactatgact tggataatac cccttttatc gatcaaattg agcaaatggg
      721 gttttacgtc gcacgctgtg cccagagcaa ttacggccaa tctgagctat ctatggcttc
      781 ttcactaaac tacgattatc tggataattt gggccggcag ttcaacccag aaaaccggcg
      841 acggactggc ttggcagact taatccagca tagcgcggtg cggtcgttat tggaagactt
      901 aggctacacc gtcgtcgcta cagacacggg ctatgacgct acccgcctga gtgacgcgga
      961 tatatacctg gccccacgtg tatcagcaga aatcaacgat ttcgaagaaa cattcctgcg
     1021 gaccactgct gcccgcctgt tcatcgaagg cgcagcaatt cttaaactac ccccagattg
     1081 ggagaaacgc gatcaagcgc accgcgagcg gatactattc caactggagc aattgagaca
     1141 aattcctgct ataccaggtc ctaagttcgt ctttgcccac atcatcaccc cacactggcc
     1201 ttacatcttc ggcccaaatg gtgagccagt ccatgaacat cccgactcga aaaccgggta
     1261 ccgcgaccag gtgatcttca tcaaccg
//
LOCUS       DBNHOH010000361         4011 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1431580, whole genome shotgun sequence.
ACCESSION   DBNHOH010000361 DBNHOH010000000
VERSION     DBNHOH010000361.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1431580"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="AC16V7_06430"
     CDS             <1..632
                     /locus_tag="AC16V7_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256791.1"
                     /translation="PLATYLSESFGIVLGNDIVVDFTSQQPFVAVANSYASHPITQKM
                     QGLVSLFPTTRSVSVTGEFNDLNRTELVLTAENAWAETDLQSLQGGEQIELQPDPGVD
                     MLGPVSLAVVIESLGKQNRLIVYGDADFANDANFMQYGNGDLLINSIDWAAGQENLIN
                     LTPKDSTQRVLIPPGRYTMGLILFGFVFLVPGAVLFSGIVVWIQRRKRG"
     gene            686..1294
                     /locus_tag="AC16V7_06435"
     CDS             686..1294
                     /locus_tag="AC16V7_06435"
                     /inference="COORDINATES: protein motif:HMM:NF025598.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HIR6256792.1"
                     /translation="MIRRPTWVLLTLFIVILAAALLWQRSEENKSASEPTPTLPARLL
                     DIDANTIRDIKIDDDQNNRLFLRKLGGGLWIMTEPERQNLDLEELNARVTQLSLINIM
                     TTLQPAPDADEIGLLPPAYTLTITDEAGKKYILDVGSETPTQSGYYVRMNGSIYVVSK
                     FNIDGMIEMLENPPIAPPTGTPTGAVAPQEVITGTAVTTTTP"
     gene            1376..2590
                     /locus_tag="AC16V7_06440"
     CDS             1376..2590
                     /locus_tag="AC16V7_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR6256793.1"
                     /translation="MIDEDLLSAIEDREEEHPAIARLIELGRQKSYVTIDDILSFFPD
                     AETDVDQLEEAFAALISAGIAYMDEPVAAGPSDDELAEEEIEEESQRDLSIDDNYLAN
                     IDTDDTIGLYLKEVGRVPLLTAREEVELAQRIEAGRLAREELARGNVSSRRRVELQLL
                     IEDGWAAREHLITANSRLVISVAKKYMGRGVPFLDLIQEGNIGLIRAAKKFDYRRGHK
                     FSTYATWWIRQAVTRAIADQGRTIRVPVHMGDQINKLLRVQHQLTQRLGRDPTVEELA
                     GALDVTPQKVENMIQVARRPLSLETPTDDEEDSVLGDFIQDEEVAAPDETATYNLLRE
                     HLDNVLNGLPPREVRILQLRYGLLDGQAYTLEEVGRKMGVTRERVRQIEAQALSRLRH
                     PAIRRKLREYLS"
     gene            complement(2650..>4011)
                     /locus_tag="AC16V7_06445"
     CDS             complement(2650..>4011)
                     /locus_tag="AC16V7_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256794.1"
                     /translation="SGKTQASFPHISLFVNAIADGITCLLLLRLGRRLGFLLAGIGAS
                     LVWAIAPYSVTFAIGGLETSLYVLLLTGAISAYLDQKFVLTMFLGSLSFLTRPDALIL
                     LGPLFVDRLILIIKVRRDLPTRLITRSLFFEGIAFLTPLLPWLIFALFYFGSPLPHSI
                     AAKSLAYRLPPEAAIIRLMQHYATPFLENLTFGTPAIRVGVVLYPFLFLVGALVAFRA
                     QRSIWPWLAFPWLYFTTFAIANPLIFRWYLTPPLPAYFLIILVGIERITHQIFNSRKS
                     RISFPNISKTRIGNILSIAFVVIAPYFLTLRGWTFHPDHGPTRPAPEMAYIQLELFYH
                     QAAESLNADISSHSSLPLLAAGDVGILGYYTGARILDTVGLNSPVSTSYFPLDPALYV
                     INYAIPPDLIIDQAPDYVVILEVYGREGLLKDPRFQAAYHLRQKIPTDMYGSDGMLIY
                     ERK"
BASE COUNT         1106 a          874 c         1071 g          960 t
ORIGIN      
        1 acccattagc gacatacctg agtgagagct ttggcatcgt tctgggaaat gatatagtcg
       61 tcgattttac ctctcagcag ccttttgtag cggtggctaa ttcttacgcc agccatccta
      121 taacccaaaa gatgcagggc ctcgtatcgc tcttcccgac aacgcgtagt gtctcggtaa
      181 caggagaatt taatgacctt aaccgaaccg agcttgtact gactgctgag aacgcgtggg
      241 cagaaaccga tttgcaatct ctgcaaggag gggagcagat tgagctacag ccggatccag
      301 gggttgatat gcttggccca gtttcgcttg cagttgtcat tgagagttta ggcaagcaaa
      361 ataggctgat cgtctacgga gatgcagact ttgccaacga tgcgaatttt atgcaatatg
      421 gcaatggcga tttgctcatc aactcgatcg attgggctgc cggacaggag aacctgatca
      481 acttgacacc taaggattca acccaacggg tactgatccc accgggaagg tacacgatgg
      541 ggctgatcct atttggtttt gtttttctag tgcctggcgc tgtgttgttc tccggtatcg
      601 ttgtctggat tcaaagacgc aagagaggtt gattcttgag tgaaccatcg acgcgccgat
      661 attgtatgtt tgtagaggaa tgtttatgat ccggcggcca acctgggtac ttttaaccct
      721 gtttatcgta attttggcag cagctttgtt atggcagcgc tcggaggaga ataaatctgc
      781 aagcgagcct acaccgacgc ttcctgcgag gctgcttgac attgatgcga atactatacg
      841 cgatataaag atagacgatg accagaataa ccgtctgttt ttgagaaagc taggcggggg
      901 tctctggatt atgaccgaac ccgaacgcca aaatcttgat ctagaagaat taaacgcgag
      961 agtcacccaa ttgagcttaa ttaacatcat gaccacttta caacccgcac cagatgccga
     1021 tgagattggt ttgctacctc cagcctatac cttgacgata actgatgaag ctggcaagaa
     1081 gtatattttg gatgtgggtt ccgaaacacc aacccaatcc gggtactatg tacgtatgaa
     1141 tggatcaatc tatgtcgtca gtaaatttaa tattgatggc atgatcgaga tgctggaaaa
     1201 tccaccgatc gcgcccccta ctgggacacc cactggtgcg gtggcacccc aagaggttat
     1261 tactgggacg gctgtaacca caaccacgcc atagtgagtg gaaattgggt attgattttg
     1321 agttaaaaag agtgtattct catattaagt tggttgaaaa tgtaatggag tctgaatgat
     1381 tgacgaagat ttgttgagtg caattgaaga tcgagaggaa gaacaccctg ctattgcgcg
     1441 cttgatcgaa ttggggcggc aaaaatcata tgtcacgatt gacgatatat tgagtttttt
     1501 ccccgatgcg gaaaccgatg tcgaccagct ggaagaggca tttgcggcat tgatcagcgc
     1561 cgggatagca tatatggatg agcctgtggc agctggtcca tccgatgatg aattggcgga
     1621 ggaagaaatc gaggaagaga gccagcgcga tctttccatc gatgacaatt atctagcaaa
     1681 tatcgacaca gatgatacga ttggactgta cttaaaagag gtcgggcgtg tacccttgct
     1741 gaccgctcgg gaagaggttg agctggctca acgtatcgaa gcgggacgct tggcacgtga
     1801 ggaactggcg aggggtaatg ttagctcacg ccggcgtgtt gagttgcaat tgttgattga
     1861 ggatggatgg gcagcacgcg aacacctgat taccgcaaat tcaagactgg taatcagtgt
     1921 tgctaaaaag tatatgggcc gtggtgtgcc ttttctagat ttgatccaag aaggaaatat
     1981 tggtctgata cgcgcggcaa aaaagttcga ttatcgtcgc ggacataaat tcagtaccta
     2041 tgcaacctgg tggatacgtc aggctgtgac gcgcgccatt gccgaccagg gtcgcacaat
     2101 ccgtgttcct gttcacatgg gagatcaaat caacaagctg ctgcgcgtac agcaccagtt
     2161 gacccaacgg cttggtcggg atccaacagt ggaggagctg gctggagcat tagacgtcac
     2221 accccagaag gttgaaaata tgatccaggt ggcgcggcgc ccactttcgc tcgagacacc
     2281 caccgacgat gaagaggatt cggtcctggg cgatttcatc caggatgagg aagtggctgc
     2341 ccctgacgaa acagctacct ataacctatt gcgtgagcat ttagacaatg tgctcaatgg
     2401 cttgcctccg cgcgaagtgc gaatccttca actgcgctat ggtctccttg acggccaagc
     2461 atatacttta gaagaggtgg gtcgtaagat gggcgttact cgcgagcgtg tgcgccagat
     2521 tgaagctcaa gcgcttagtc gcttgcgaca cccggctatt cgtcggaaat tgcgcgaata
     2581 tctgagctga caatacgggt tatttattca gacagggcta tgtggactta attcccccat
     2641 agccctgttt tactttcttt cgtagatgag catcccatcg cttccataca tgtcagttgg
     2701 aattttttgg cgaagatgat atgctgcctg gaagcgcgga tctttaagca agccttctcg
     2761 accatacacc tccaagatga cgacataatc gggagcctga tcgatgatga ggtctggcgg
     2821 gatagcgtag ttgattacat ataaagctgg gtcgagcggg aagtagctgg tggagacggg
     2881 tgagttcaac ccgaccgtat caaggatgcg cgcaccggtg taataaccca atatacccac
     2941 atcccccgcg gccaataaag gcagactaga atgcgaagaa atatctgcat tgagggattc
     3001 agcagcttgg tggtagaaaa gttcaagttg tatgtaagcc atctctggcg caggacgagt
     3061 tggaccatga tcgggatgaa acgtccatcc tctcaaggtc aaaaaatatg gggcaattac
     3121 cacgaaagcg atagaaagga tatttcctat acgtgttttt gaaatgttcg gaaaagaaat
     3181 tcgagatttt cgggagttaa atatctggtg ggtgattcgt tcaataccta ccaggattat
     3241 tagaaagtaa gcgggtaacg gtggggtgag gtaccaacga aagatgagcg ggttggcaat
     3301 ggcaaacgtg gtgaaataaa gccacggaaa tgccagccaa ggccatatcg atcgctgtgc
     3361 tcgaaatgca accagagctc ccaccaaaaa cagaaaggga tacaaaacta ccccaaccct
     3421 aattgccggg gtaccgaaag tcaggttttc caggaaaggc gtggcgtagt gctgcatcaa
     3481 tcggataata gccgcctccg gtggtaatcg ataagccaag ctttttgccg ctatggaatg
     3541 aggcagcggg ctgccaaaat aaaatagagc gaaaattagc cagggtaata agggggttaa
     3601 aaatgctatg ccttcaaaaa atagcgaccg cgtgatgagc ctagttggta aatcacggcg
     3661 cacctttatg ataagaataa gacgatcgac aaataatggt cccaacagaa ttaaagcatc
     3721 tgggcgggtg agaaatgata gggagcccaa gaacattgtc aaaacaaact tctggtctaa
     3781 atatgcactt attgcgccag taagcagaag aacatacagg ctggtttcca ggccgccgat
     3841 cgcaaaggtc acgctgtagg gtgcgatagc ccagaccaat gatgcaccga tccctgccag
     3901 aaggaatcct agcctacgac ctagtctgag caatagcaga catgtgattc catcagctat
     3961 ggcgttcaca aataaggata tatggggaaa agatgcctgg gtttttcctg a
//
LOCUS       DBNHOH010000362         2469 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1110158, whole genome shotgun sequence.
ACCESSION   DBNHOH010000362 DBNHOH010000000
VERSION     DBNHOH010000362.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1110158"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="AC16V7_06450"
     CDS             complement(<1..840)
                     /locus_tag="AC16V7_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256795.1"
                     /translation="MSVIWVKVWFDLWHNKLRTLLVILSIAVGVFAVGTTFGLAEQML
                     PTMDAAHQMTLPAHVTMALMQPVDRDSLLALSDVPEVEEVEVLNMIEVRYKLRPGDKW
                     RKGVIMSRDDYESEVYELLQLKAGSWPEGKYLNIERMHAPFYGIDVGDKVIFEVGKRE
                     RTFEINGKIRHPFVPPPTMYDMAYFFGGEDTMEEYGIPRGQFTRIMFRVTPYTDDHAR
                     EVASVVKERLAKQGVSVAATMYQDPNKHWGRAFVDGMALVTKVLAVISMLLSAVLVSN
                     TLMA"
     gene            complement(837..1823)
                     /locus_tag="AC16V7_06455"
     CDS             complement(837..1823)
                     /locus_tag="AC16V7_06455"
                     /inference="COORDINATES: protein motif:HMM:NF014036.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HIR6256796.1"
                     /translation="MPTNLPPDYFEVDKRFREAQSPAEKVALLEELIATVPKHKGTDK
                     LRADLRRQLSKLREEAQVRKKQGAHTSAFQIEREGAGQAVLIGPTNVGKSALVAALTR
                     AEPDVSPAPYTTWKPTPGMMMVENIQVQLVDTPPMDSGYVEPGLFDLVRRSDLVLLVV
                     DLQADPLNQLERTLTLLEEHRIAPAHLKDTYPQDSRMFFLPVIVLANKCDDEHLDELF
                     EVCCEIMEGDWTTLPVSAVTGRNFELLKRTVFERLDIMRVYAKPPGNEADLERPFVLK
                     KGSTITDLAQKIHRDFYDHLKSARGWGSSTFDGQMVQRDYELQDGDIVELRV"
     gene            complement(2130..>2469)
                     /locus_tag="AC16V7_06460"
     CDS             complement(2130..>2469)
                     /locus_tag="AC16V7_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256797.1"
                     /translation="GVLYLIGAIFKSLYTLRHGDEFYGSFADKAILAPSKQLIRKLVI
                     PQARAFTILLIVFQLTVAFCILSRGALVRPGLMAGAVFSFAVVFVSNTSGAIANLVLP
                     VVQAYLALIR"
BASE COUNT          603 a          708 c          596 g          562 t
ORIGIN      
        1 cgccatcagc gtattggaaa ccagcactgc gctgagcaac atggagatca ccgccagcac
       61 ttttgtgacc agcgccatac catccacgaa ggcacgtccc caatgcttat tcggatcctg
      121 gtacatggta gccgcgacgc taaccccctg cttggccagg cgctccttga ccaccgaggc
      181 cacctcacgg gcgtgatcgt cagtataagg agtcacgcgg aacataatgc gggtaaactg
      241 gccgcgcggg atgccgtact cctccatggt atcttcacca ccgaaaaaat aggccatatc
      301 gtacatcgtg ggcggcggca caaatggatg gcgaatcttg ccgttgatct cgaaagttcg
      361 ttctcgcttg ccgacctcga aaatcacctt atcgccgaca tcaatgccat aaaacggcgc
      421 atgcatgcgc tcaatgttca gatacttgcc ctccggccag gaacccgctt taagctgcaa
      481 tagctcatat acttcactct cgtagtcatc gcggctcatg atcacgcctt tgcgccactt
      541 gtcgcccggg cgcagcttat aacgcacttc gatcatgttt aagacctcca cctcctcaac
      601 ctcgggcaca tcgctcaacg ccaggaggct atcacggtct acgggttgca tcagtgccat
      661 cgtgacgtgg gcgggcaagg tcatttggtg ggctgcgtcc atggtgggca gcatctgttc
      721 cgccaggccg aaggtagtgc ccactgcgaa gacgcccacc gcaatgctca gaatgacgag
      781 cagtgtgcgc agcttgttat gccacagatc aaaccaaact tttacccaaa tgacactcat
      841 acgcgcaact ccacaatatc tccatcttgc agttcataat cgcgctgcac catctgccca
      901 tcaaaggtac tgctgcccca cccgcgcgcc gattttagat gatcatagaa atcacgatgg
      961 attttttgag ccagatcagt aatcgtgctt ccctttttta acacaaatgg tctctcgagg
     1021 tcggcttcat tgccgggtgg tttggcatac acccgcatga tgtccaagcg ctcaaagacg
     1081 gtgcgtttca aaagctcaaa attacgaccc gtgactgctg acaccggcag ggttgtccaa
     1141 tctccttcca taatctcgca acacacttca aatagctcat ccaggtgctc atcgtcacac
     1201 ttgttggcta gcacgatcac cgggagaaag aacatgcggc tgtcctgcgg ataggtgtct
     1261 ttcaaatggg caggcgcaat acgatgctct tccagcaagg tcagggtgcg ctcaagctga
     1321 tttaacggat ccgcttgcag gtcgaccacc agcaaaacca gatccgatcg gcgcaccagg
     1381 tcgaacaagc cgggttcgac gtaaccgctg tccataggcg gcgtatccac cagttggacc
     1441 tggatatttt caaccatcat catgcctggg gttggcttcc aggtggtgta aggcgcgggt
     1501 gaaacgtccg gctcagcccg ggtcagcgcc gccacgagcg cagatttccc cacgttggtt
     1561 ggaccgatca acaccgcctg accagccccc tcccgttcga tttgaaaagc cgaggtatgc
     1621 gcgccttgct ttttgcggac ctgggcttcc tctcgcagtt tggatagctg gcggcgcagg
     1681 tctgcgcgca gcttatcggt gcccttgtgc ttcggtacgg tagcgatcag ctcttccagc
     1741 aaagccactt tttccgccgg ggattgtgcc tcacggaagc gcttatccac ctcgaaataa
     1801 tctggaggta ggtttgtagg catggcttat tgttaaatta tatacaattt ccgcctactt
     1861 taggcaaatg tcttgataaa tgcgtcattg tgggtaatct gacagcgcag ggcacacttc
     1921 aatctcaggc gaaatcgacg gttatcgcga cacctggcaa aaaaagatat gtctgggcga
     1981 tttcagataa agattgagat ggcttaatcg ctgcccaccc gggccagctc gaccaatgct
     2041 actggctaag caacaacccc cacatatagc ccttactaag gcaagttcag aagaaccata
     2101 ctatatgttg cctaaggatt atgaattttc taccggataa gtgcaagata cgcctgaacg
     2161 acgggcaaca ccagattagc tatcgcacca gaagtattcg agacaaacac aactgcgaag
     2221 ctaaacactg ccccagccat tagtcctggc cttacgagtg ctcccctgct caagatgcag
     2281 aacgccactg tcaattgaaa gacgatcagc aatatcgtga acgccctcgc ttgaggaatt
     2341 accaatttcc gaattaactg ttttgaagga gctaggatgg ctttatctgc aaaacttcca
     2401 taaaactcat caccgtgacg cagggtatag agggacttga aaatagcccc tattagatac
     2461 agcactcca
//
LOCUS       DBNHOH010000363         1840 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_470075, whole genome shotgun sequence.
ACCESSION   DBNHOH010000363 DBNHOH010000000
VERSION     DBNHOH010000363.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_470075"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="AC16V7_06465"
     CDS             complement(<1..895)
                     /locus_tag="AC16V7_06465"
                     /inference="COORDINATES: protein motif:HMM:NF014476.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HIR6256798.1"
                     /translation="MLILNADDVRKALPMAQAIEAMKTAFAALSDGRAEVPLRTQLPI
                     KSRNAVSLFMPAYVLDEKGESLVVKIVSLFPNNALHGLPLIHAAVLVLEANTGRTIAL
                     LEGGTLTAIRTGAASGAATDLLAKPDARVVAIFGSGVQGRTQLEAVCSARSIQTAWIY
                     DRNPERRDEFIAEMTGQEDIPADLRPAESPSQALEDADVVCTATTSKRPIFDDADLKA
                     GVHINGVGSYTPEMQEIPAETVRRALVVVDSRSASMVEAGDLLKAILLGYITAKHIHG
                     ELGEIVLGRKPGRTNPDQVTFF"
     gene            complement(935..>1840)
                     /locus_tag="AC16V7_06470"
     CDS             complement(935..>1840)
                     /locus_tag="AC16V7_06470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6256799.1"
                     /translation="AVQPPDVEGLKANIRLQQSVGIQTSFVSPQEIKEIDPHADVAGI
                     GGAAYEPEGGYCDAATAANAFAQAAQRLGTRMEIGVETTRIQVESGRVVGVETSQGFY
                     PAPHVVVVAGPWTPRLLSHLGVEFPSIAARIKIGFYRRPAQFDRHCVWGDFANQIYLR
                     PETGNLTLVGSISPDEANDTVADPDNFRETVELEVLADFAERAVKRFPALEQSHVLRS
                     YAALYDVTPDWHPILDGLPGVEGLYVCAGSSGHGFKLAPAAGKMMAKLIMDGKKQDDD
                     IELFSFERFEKGSLVRGQYAYSIVG"
BASE COUNT          401 a          528 c          475 g          436 t
ORIGIN      
        1 tgaagaatgt cacttgatct gggttggttc gaccaggttt gcggcctagc acgatctcgc
       61 cgagttcgcc gtggatatgt ttggcggtga tgtaacccag aagtattgct tttaacaagt
      121 ctcctgcttc gaccatggac gctgagcgtg aatctacaac caccagagcg cgccgaactg
      181 tctctgcggg aatctcttgc atctcgggtg tgtatgaccc gaccccgttg atatgcacgc
      241 cggctttcag gtctgcgtca tcgaagatcg gtctcttaga tgttgtcgcc gtacagacca
      301 catccgcatc ttctagcgct tgggacgggc tttccgccgg gcgcagatcg gctgggatgt
      361 cttcctgccc tgtcatctct gcaataaatt catccctgcg ctcagggttt ctatcgtata
      421 tccaggctgt ctggatcgat ctcgccgagc agaccgcctc caattgggtt cgcccctgca
      481 cgcccgagcc aaaaatagca accacgcgcg catccggttt cgccagcaaa tcggtagcgg
      541 caccggatgc agccccggtt cgtatggcgg tgagcgtgcc cccttccagc aaggcgatag
      601 ttcgcccggt gttagcttca agcaccagaa cggcagcgtg gatgagtggc aaaccatgca
      661 aagcgttgtt tgggaagaga gatacaatct tgacaaccaa ggattcgccc ttctcatcca
      721 aaacataggc aggcataaac aggctcactg cattgcggga ttttatgggc aattgtgtcc
      781 gcagcggtac ctcagcgcgc ccatccgaga gagccgcgaa agcggttttc attgcttcga
      841 tggcttgtgc cattggaagt gcttttcgaa catcatcagc gttcaggata agcatgtcaa
      901 catcctcttc caaacccttt gctttggtca gtcatcatcc aacgatactg taagcatatt
      961 gcccacgaac caaactcccc ttttcaaagc gctcaaatga gaataattcg atatcgtcgt
     1021 cctgtttttt gccatccata atcagcttcg ccatcatttt ccctgcagca ggtgccagtt
     1081 tgaagccatg cccgctgctg ccggcacaga catataatcc ctcaacccct ggtaagccgt
     1141 cgagtatcgg gtgccaatca ggcgtgacat cgtataaggc tgcataactg cgcaaaacgt
     1201 ggctctgttc tagggcaggg aaacgcttga cggcgcgttc tgcgaaatca gccaagactt
     1261 ccagctcgac cgtttcacga aaattatccg ggtcagctac tgtgtcattg gcttcatcgg
     1321 gtgaaatcga gccaaccaag gtcaggttgc ccgtctcagg tcgcaagtag atctggttcg
     1381 caaagtcgcc ccatacgcaa tgccggtcaa attgtgcggg tcggcgatag aagccgatct
     1441 tgatacgtgc ggcaattgat gggaattcaa cacccaggtg cgagagcagc cggggtgtcc
     1501 aaggaccagc caccaccacc acgtgaggag cggggtaaaa tccctggcta gtctcaacac
     1561 caaccactcg accactttca acctgtatgc gggttgtctc taccccaatt tccatgcgtg
     1621 tgcccaagcg ctgagctgcc tgagcaaagg cgttggctgc cgtggctgca tcgcaatagc
     1681 cgccctcggg ctcgtaagcc gctcctccaa ttcccgccac atcagcgtga gggtcaatct
     1741 ctttgatctc ttgtggggag acaaaacttg tttgtatgcc caccgattgt tgcaggcgaa
     1801 tattggcttt caggccttcc acatcgggtg gctgtaccgc
//
LOCUS       DBNHOH010000364         7294 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_405501, whole genome shotgun sequence.
ACCESSION   DBNHOH010000364 DBNHOH010000000
VERSION     DBNHOH010000364.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7294)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7294)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7294
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_405501"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="AC16V7_06475"
     CDS             complement(<1..339)
                     /locus_tag="AC16V7_06475"
                     /inference="COORDINATES: protein motif:HMM:NF015013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HIR6256800.1"
                     /translation="MGYTIGMSHIARSSLIIAVFFGLDKILGFLRQVFFNRQFSAAER
                     DVFFVSNNIPDLLSALISGGALGIALIPILSEFLERDGRQAAWDLFSRIVNLAFLVTA
                     CISVVIILLAE"
     gene            417..1424
                     /locus_tag="AC16V7_06480"
     CDS             417..1424
                     /locus_tag="AC16V7_06480"
                     /inference="COORDINATES: protein motif:HMM:NF027484.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HIR6256801.1"
                     /translation="MPLKKILNFVLLSSLLVFSGCKPGGQAEATETPLSPEAVFTAAA
                     LTAQVRMTELIASTPSPQPATPTQTLAAATVTSTVPSAGAAPSLTLPPAVPGADKVEF
                     VADLTIPDGTVFSPGEKFTKTWRLMNAGTATWTTAYTLVFFSGEQMGGAASTSLPLEV
                     PSGQTLDLSVQLTAPAQAGSYTGYWILRNAAGRNFGLGPNADGPFYVQINVSGTAATG
                     TPQTGTPSASSTPGGDTVSGVTLGVDAATVQQACPYTFTFSGRFTLNKTATVTYRLEA
                     DTSFPINLPGATTTSLNAGTHEVTYTLEFSDSVTGTARLHVTAPQDVNSDPVSFSLTC
                     N"
     gene            1485..2729
                     /gene="queG"
                     /locus_tag="AC16V7_06485"
     CDS             1485..2729
                     /gene="queG"
                     /locus_tag="AC16V7_06485"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HIR6256802.1"
                     /translation="MSLTQAIKQQAQTLGFQLVGITTPDPPPHYPAYEGWLQAGRHGE
                     MGYLASESARQRRRDPRQILPECRSILVLGIRYPAPTHPFPGGKEQIDIPLVKGSVAA
                     LDQRTSFPLPKVMGEGVGGGGYQQNAGRVASYAWGDDYHNVLATRMRSLVAFIETQLG
                     GPFPNRWYTDTGPILERDLAQRAGLGWIGKNTCLIAPSLGSYFFLAEILLGIELEPDP
                     PFLPDYCGSCTRCIEACPTACILPDRTLDATRCISYLTIELKGVIPLDLRPQMGNWIF
                     GCDICQQVCPWNQRFADPEGDSDFRPRPGVPDPDLIPELALNPQEFNRKFKDNPVKRA
                     KRRGYLRNVAVALGNTHHTDAISPLIQALEGDAEPLVRIHAAWALGQIGGKEARLGLQ
                     SALQSEADNEVRAEILVALEMV"
     gene            2738..3910
                     /locus_tag="AC16V7_06490"
     CDS             2738..3910
                     /locus_tag="AC16V7_06490"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HIR6256803.1"
                     /translation="MPRLAALFLLTVFLLSACQSQQASIPSTGDPLTANSTPTSSSPP
                     PLSLTPLAPILSTSLPSTNTPSPSPSPTPTQAPRSPTPPPTTTLLFTGVIVPARCVQA
                     AIDASGDPDYPYDEVREVITKADLAVGTLNATMSDYPPHTGCKSTYVLVGNPENADAL
                     GRAGFDLMSVATNHIKNCGLTNCGDRAFFDTLDNLRRVGIQPIGAGANHTEAMQPVVV
                     TLNGVRFGFVSLGQIEPMAFATDERPGIAVLNEDNLRSAIAAARKVSDVVIAMPHWGP
                     EDVPTPNWSQRGLAQQAVEAGADLVVGNHTHVVQAMQQIDGVQVFYGLGNFVFDQWPR
                     DHQQGVILLVRFIGTRYDGYEFIPTHVDRDGRVHMANQDEADEILERIRQASEKLK"
     gene            3972..4766
                     /locus_tag="AC16V7_06495"
     CDS             3972..4766
                     /locus_tag="AC16V7_06495"
                     /inference="COORDINATES: protein motif:HMM:NF028519.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR6256804.1"
                     /translation="MNRRHIYASLYLLGLLIVLVGLGAPALAAPNLQLTPFPTPTPGP
                     DGRIVYFVQEGDTLLRISLISGITVDELRGLNNLTGDNIVVGQELLLGLGGPSQPSPS
                     PGPSPTVTPPLPTPSPQPGLANLCVILYDDINGDSIRQEEEPFIPGGAISVNNRSGSV
                     AETVQTPSGLEPYCFEELPEGEFSISVAIPEGYNPTTVTNYAMRLNAGDETFLDFGAQ
                     ANTETIAEAPTPTGSGRSPILGIIGGLFLLAGAGLAFFASRLMRSK"
     gene            complement(4809..5921)
                     /locus_tag="AC16V7_06500"
     CDS             complement(4809..5921)
                     /locus_tag="AC16V7_06500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6256805.1"
                     /translation="MTLPGTLLHSVLYDGWSLIYQPNSPQSLHLLAILSCRLEGFQGF
                     LALPGEPPDWLPPVETTRVIFTANMPRNRLVWEQRILPGLRRELEADLLHLTSPTPPL
                     FGPAVNVVSPSGFGGSHRVGGFSSRLRQAVSQGGMARLAGLFWPDDLPDPKGMGAVYR
                     LPPAIPVEFIDLGSKPGDRQLGESLDLPEAYVLYHGPAEETALRRLLNAWSWVAGSVG
                     EMHPLLAIGLEDESRKKLDVLAASFDLSETVRSLPAMPPKDLGLIYQRCVAVLHPGDD
                     SPWGGPVRLALASGKPVVSQVSEHMDALVGPAAYLVKGGEARALGAALITVLVEEEIA
                     ERLSQLGRQRAEAWSLETFSQGLLAAYQDMLSKQNK"
     gene            complement(5918..6334)
                     /locus_tag="AC16V7_06505"
     CDS             complement(5918..6334)
                     /locus_tag="AC16V7_06505"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF046335.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HIR6256806.1"
                     /translation="MDLNQHIQPGLFREDTYFVEEHQSAIHVGSGSLRVLATPWMIAF
                     MEKTARLLLSERLPAGYSSVGTHVDVRHLAPSAVGARLRVRAEVLSVDALKVAFAVQA
                     WDEQEQIGAGQHQRVVIDEERFLRRIAAKTSKAEGS"
     gene            complement(6353..>7294)
                     /locus_tag="AC16V7_06510"
     CDS             complement(6353..>7294)
                     /locus_tag="AC16V7_06510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6256807.1"
                     /translation="LQSLRDYELVAVDDGSNDSTPEKLRQRAENDPRLRVLALPHGGI
                     IAALNAGLAACRGEYVARMDADDRAHPARLEKQLIYLEDHPEVAVLGCRVAGFPPDQV
                     REGFRIYLQWQNALLSDAEIRREMFVESPVAHPSVMVRRQWLEEVGGYQEHGWAEDYD
                     LWLRLYLAGARFAKLPEILLEWRERPERLTRRDGRYSLENFLRAKAFYLGKGPLAGRD
                     AVMIWGAGMIGRRLSKHLLRQDVPLVAFIDIDPRKIGRLRRDRPILSPDELPDWWGRY
                     QNPVVLAAVGARGARALIRQRLNAMSLCEGRDWWGVA"
BASE COUNT         1594 a         2386 c         1900 g         1414 t
ORIGIN      
        1 ctcagccagc aagatgatga ccacagagat gcatgctgtg accagaaaag ccaggttgac
       61 gatgcgagag aacaggtccc aggccgcttg gcgaccgtcg cgctccagaa attcagacaa
      121 gatcgggatc agtgcaattc ccagcgcgcc accggagatt aaggcagaga gcaggtcggg
      181 gatgttattg gagacaaaga aaacatctcg ctcggcggcg ctgaactggc ggttgaagaa
      241 cacctgtcga agaaatccca ggatcttatc cagaccaaag aatacagcaa tgatcagcga
      301 cgagcgggcg atgtgcgaca tgccgatagt ataccccacc taaacaactc ccctgcgccc
      361 taatttcaag cttaccagtt atacttgggg cgtggaaatt acctggaggt aagatcgtgc
      421 ctttgaaaaa aatactcaac ttcgttttat tgtcatctct cctggtcttc tcaggctgca
      481 aaccgggggg gcaagctgaa gcaaccgaga cacccctcag ccctgaagcc gtattcactg
      541 cggcggcctt aaccgcccag gtcaggatga ctgagctgat tgccagcacc ccctcccccc
      601 agccagccac gcccacgcaa actctcgctg cagcaacggt aacgtctacc gtgccttcgg
      661 ctggtgccgc acctagcctc accttgcctc ccgctgtgcc gggagcagac aaggttgagt
      721 tcgtcgccga tttgaccata cccgacggca cagttttctc acctggtgaa aaattcacca
      781 agacctggcg cttgatgaat gccggcacag ctacctggac aactgcctat accctggttt
      841 tcttcagcgg ggagcaaatg ggtggtgcgg catctacctc cttacccctg gaggtgccat
      901 ctggacaaac cttggacctc agcgtacaac tcaccgcccc cgcccaggct ggttcgtaca
      961 ccggttattg gatcctccgc aatgctgccg gaagaaattt tgggctggga cctaacgcag
     1021 atgggccatt ctatgtgcag atcaacgtct cggggacagc cgccaccggc acaccccaga
     1081 ccggcacgcc atccgcctca tccacaccag gaggagatac cgtcagcggt gttaccctgg
     1141 gcgttgacgc ggcaacggta caacaggcct gcccctacac tttcaccttt tctggacggt
     1201 tcaccctaaa caaaaccgcc acggtcactt accggctcga agcggatacc agcttcccga
     1261 tcaatctacc tggagcgacc accacttcac tgaatgccgg gacgcacgaa gtcacctata
     1321 ccctggaatt cagcgattcg gtcacgggga cagcccggct gcacgtcact gccccacaag
     1381 atgtgaattc ggatccggtt agcttctcgc tgacctgcaa ttaagctgtg tttttggtct
     1441 gtgggcatct atatctctca tagtctatcg tttcatttgc aaccatgtcc ctcacccagg
     1501 caatcaaaca gcaggctcaa acattgggtt ttcaattggt tggcatcacc acgcctgatc
     1561 ctccgcctca ttacccggcc tacgagggct ggttgcaagc cggccggcac ggcgagatgg
     1621 gctacctggc aagcgagagt gcccgccagc gccgccgcga cccgcgccag atcctgcctg
     1681 aatgccgctc aattctggtg ctgggcatac gttatccagc ccccacccac ccgttcccag
     1741 ggggaaaaga gcaaatagat atcccgctgg tcaagggcag cgtcgctgcc cttgaccagc
     1801 ggacttcttt cccccttccc aaggttatgg gggagggggt agggggtggg ggataccagc
     1861 aaaatgccgg ccgtgtcgca tcttacgcct ggggcgacga ttatcacaac gtactggcga
     1921 cgcgcatgcg ctccctggtg gctttcatcg agacgcagct gggaggcccg tttccaaacc
     1981 gctggtacac cgacaccggc cccatcctgg agcgcgacct ggcacaacgc gccggtctgg
     2041 ggtggatcgg caaaaacacc tgcctgatcg cacccagcct gggatcgtat ttctttctgg
     2101 cagagatcct cctggggatc gagctggagc ccgatccgcc cttcctgccg gattattgcg
     2161 gctcgtgcac gcgctgtatc gaagcctgtc caaccgcctg cattttgccc gatcgcacct
     2221 tggatgctac ccgctgcatc tcgtacctga ccatcgagct aaaaggcgtt atcccgcttg
     2281 acttgcgccc gcaaatgggc aattggatct ttggttgtga tatctgccag caagtctgtc
     2341 cgtggaacca gcgcttcgct gatcctgaag gcgactcgga ctttagacca cgcccgggcg
     2401 tgccggaccc ggacttgatc cccgagctgg ctctcaaccc ccaggagttc aaccgaaagt
     2461 ttaaggataa cccggtaaaa cgcgccaaac gacgcggcta cctgcgcaat gtagcggtcg
     2521 ctctgggcaa cacacatcat actgacgcca tctcccccct aatccaagcc ctggaaggcg
     2581 acgcagaacc cctggtgcgc atccacgccg cctgggcctt ggggcaaatc ggcggcaaag
     2641 aagcccgcct ggggctgcag tctgccttgc aaagcgaagc agataacgag gtgcgtgctg
     2701 aaattcttgt ggcattagaa atggtataac tcgcagcatg cctcgattgg ccgccctctt
     2761 ccttctcaca gtatttctgc tatcagcctg tcagtcccag caagcgagca tcccttcgac
     2821 tggagaccca ttaacggcga attccacccc cacatcctca tcccctcctc ccttatccct
     2881 tactccctta gcccctatcc tctcaacctc tcttccgtcc acgaacaccc cttctccttc
     2941 tccctctccc actccaaccc aggcaccccg cagccccact ccaccaccca caacaacgct
     3001 gctctttacc ggcgtgatcg tgccagcgcg ctgcgttcaa gctgccatcg atgcctccgg
     3061 agacccagac tacccctacg atgaggttcg agaggtcatc accaaagcag acctggcggt
     3121 cggcacgctc aatgccacca tgagcgacta cccgccgcat accggctgca aatcgaccta
     3181 tgttttggtg ggcaaccccg aaaacgccga cgccttaggt cgtgctggtt tcgacctgat
     3241 gagcgtggct accaatcaca ttaaaaactg cggcctgacc aactgcggtg accgcgcctt
     3301 ttttgacacg ttggacaacc tgcgtcgggt gggtatccag ccgataggtg ccggggcaaa
     3361 tcacaccgag gccatgcagc cggtggtagt gacactcaac ggcgtgcgct ttggtttcgt
     3421 gtcgctggga cagatcgagc caatggcctt tgccaccgac gaacgaccgg gcatcgccgt
     3481 cctcaatgag gataacctgc gcagcgcaat tgccgctgcc cgcaaagtct cagatgtggt
     3541 cattgccatg ccccactggg ggccggagga cgttcccaca ccgaactggt cgcagcgcgg
     3601 gctggcccaa caggcggttg aagcgggcgc tgacctggtg gtcggcaacc acacccacgt
     3661 cgtacaggca atgcagcaaa tcgatggcgt gcaggtcttt tacgggctgg gaaatttcgt
     3721 cttcgaccaa tggccgcgcg accaccagca aggtgtgatt ctgctggtgc gcttcattgg
     3781 cactcgctac gatggctacg agttcatacc cacccatgtg gaccgcgacg ggcgagtcca
     3841 catggcaaat caagacgagg ctgacgagat tctagagcgc atccgtcagg cgagtgaaaa
     3901 actcaaatag ttcataggca actaccctaa tacaggcata ctccggtgat aatttccgtt
     3961 ataatctctt tatgaaccgg agacacatct acgccagtct ctaccttcta ggtctgttaa
     4021 tcgtcctggt tggcctgggc gcgccggccc tggcagcgcc aaacctgcag ctaaccccct
     4081 tccccacccc cacacctggc ccggacgggc gcatcgtcta ctttgtccag gagggtgata
     4141 ccttgttacg catctccctc atctccggga tcacggtgga cgagctgcgc gggttgaaca
     4201 atctcaccgg ggataatatt gttgtcgggc aagaactgct actcggtctg ggcggcccat
     4261 cccagccttc accctccccg ggccccagcc ccaccgtcac accacccttg cctaccccct
     4321 caccacagcc cgggctggcg aacctgtgtg tcattctcta tgacgacatc aatggcgact
     4381 ctatccgcca ggaggaagag ccatttatcc cgggtggcgc catcagcgtg aacaatcgct
     4441 ctgggagcgt cgcagagacc gtccagacac ccagcgggct ggaaccctac tgcttcgagg
     4501 aactgcccga aggagaattt tcaatctcgg tcgcaatacc agagggctac aaccccacca
     4561 ccgtgaccaa ttacgccatg cgcctgaacg caggtgatga gaccttcttg gattttggag
     4621 cgcaagccaa tacagagact atcgctgagg cacccactcc aaccggcagc ggcagatcgc
     4681 ctatattggg catcattggt ggactgttcc tattagcagg tgccgggctg gcgttcttcg
     4741 catcccgctt aatgcgaagc aaataaaata caatacatga aaaaacccca caatttctca
     4801 ttgtggggtt atttgttttg cttgctaagc atatcctgat atgccgccaa cagcccctgg
     4861 gaaaaagtct ccagcgacca ggcctcagcc cgctggcgtc ccagctggga tagccgctcc
     4921 gcaatctctt cttctaccag cacagtgatt aaagccgccc ccagggcacg cgcctcacca
     4981 cccttcacaa gataagccgc tgggccgacc agtgcgtcca tgtgctcgct cacttgagac
     5041 acaaccggct tcccgctcgc cagagccaaa cgcaccggcc cgccccaagg agagtcatcg
     5101 ccgggatgta agacagcgac acagcgctgg tatatgagcc ccaagtcttt aggaggcatc
     5161 gccggcaggg aacgcactgt ctcgctaaga tcaaagctcg cagccagaac atccagcttt
     5221 ttccgacttt cgtcctccaa tccaatcgct aatagagggt gcatctcacc taccgagcca
     5281 gccacccagc tccaggcatt taacaagcgg cgcagggcgg tttcttctgc cggtccgtga
     5341 tacaacacgt aggcctccgg caggtcaagc gactcaccca gctggcggtc acctggcttc
     5401 gaaccgaggt caataaattc gacagggatc gcagggggta gccggtaaac cgctcccatc
     5461 ccttttggat ccggcaaatc atctggccaa aacaaaccag ccaggcgtgc catgcctccc
     5521 tgggaaaccg cctggcgcag tcgggagctg aagccgccta cgcggtggct gccgccaaat
     5581 ccgctcgggc tgaccacatt gactgcaggt ccaaaaagtg gcggggtagg cgaagttaga
     5641 tgcaggaggt ctgcctccaa ttcacgccgc agaccaggca gtatgcgttg ctcccacact
     5701 agccggttgc gtggcatatt ggcggtgaaa atcacccggg tagtttcgac gggtggaagc
     5761 cagtcgggcg gctccccggg caatgccagg aagccctgga agccctccaa acggcaagat
     5821 aggatagcca gcaagtgcag agattgcgga ctatttggct ggtaaatcag tgaccagcca
     5881 tcatacaaga cggaatgaag cagtgtgcca ggcaaggtca cgaaccttct gcttttgatg
     5941 tttttgccgc gatacgccgc aggaagcgct cttcatcgat cactacgcgt tggtgctgcc
     6001 ccgcaccaat ttgctcctgt tcatcccaag cctggacagc aaaggcaact ttcagagcat
     6061 ccaccgacag cacttcagca cgcactcgca ggcgcgcccc cactgccgag ggagccaggt
     6121 ggcgcacatc cacatgagtt cccacgctgg aataacccgc aggcaggcgc tcgctcagca
     6181 acaggcgagc tgttttttcc ataaaggcga tcatccaggg tgtagccagc acgcgcaacg
     6241 agccgctacc cacatggatg gcggactgat gctcttcgac aaaataggtg tcctcgcgga
     6301 acagaccagg ttggatatgt tgattaagat ccatacttgt ccaatatctc ctttacgcca
     6361 ccccccacca atcccgcccc tcgcacaggc tcatggcgtt caagcgctgt cggatcagcg
     6421 ctctcgcccc acgcgccccc accgccgcca gcacaacggg gttttggtag cgtccccacc
     6481 agtcaggcaa ctcgtcagga gaaaggatag ggcggtctcg cctcaggcgc ccgatcttac
     6541 gagggtcgat atcaataaag gccaccagcg ggacatcttg gcgaagcagg tgcttactca
     6601 accgtcgacc gatcatgccc gctccccaga tcatcacggc atccctgcct gcaagcggcc
     6661 ctttccccag gtaaaatgcc ttggcacgca ggaagttctc caaagagtaa cgcccatcgc
     6721 ggcgcgtcag ccgctccggt cgctcgcgcc actcgaggag aatttcgggt agcttggcga
     6781 agcgcgcccc cgccagatac aggcgcaacc acaggtcata atcctccgcc cagccgtgct
     6841 cctgataacc gccaacctcc tccaaccact gtcgccggac catcacgctg gggtgcgcca
     6901 cagggctttc gacaaacatc tcacgccgta tctcggcatc gctcagcaaa gcattctgcc
     6961 actgcaggta aatccggaat ccttcgcgca cctgatctgg cgggaatccc gctacccggc
     7021 agcccaacac agccacttca gggtgatcct cgaggtatat gagctgcttc tccagccgag
     7081 cagggtgagc gcgatcgtcg gcgtccatac gggcgacgta ctcaccccgg caagctgcca
     7141 gacccgcgtt cagcgcagcg atgatacccc catgcggcag cgccaggacc cgcaggcgcg
     7201 ggtcattctc agcgcgctgg cgcagcttct ccggcgtgct atcattcgag ccgtcatcca
     7261 cagccaccag ctcataatcc cgcaggctct gtaa
//
LOCUS       DBNHOH010000365         2245 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_213420, whole genome shotgun sequence.
ACCESSION   DBNHOH010000365 DBNHOH010000000
VERSION     DBNHOH010000365.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_213420"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="AC16V7_06515"
     CDS             <1..336
                     /locus_tag="AC16V7_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256808.1"
                     /translation="VRPMVHQWFEARYSPSIAIVLISLALLVIKLTPRTTLHPLARIL
                     LCTGAGFLGFGFFRVTLGLVYAEALVWANFWEELTELMFVVAVIYTLWVFRRTLLPDN
                     KFLETHTSL"
     gene            701..1990
                     /locus_tag="AC16V7_06520"
     CDS             701..1990
                     /locus_tag="AC16V7_06520"
                     /inference="COORDINATES: protein motif:HMM:NF013226.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HIR6256809.1"
                     /translation="MKHTEVKSTITCDMEGRIETYNKGAEEIFGYSAEEVIGHKRVSL
                     FSPGLIVLEHVPTWLKTASETGEYKGQTVFVRKDGSTFNAEIRITPTFRDGKQVGYCG
                     VTKPLDPSQRANAMPKISMLTKVFAGMVITRMPFLTASLIPVLIGATWAVVARSASPF
                     PWLVFILTLLGAAALHVAANTFNDYFDWTSGTDKVNNDYFLPFSGGSRSMELGLISEK
                     GVLNVGLVALGLAFGFGLTVMFLSQQPLLILFGLAGAFSAYFYTAPPLRLVARKGLGE
                     LLIGLNFGPLMTAGTVLALTGRVAWEDFVIGLPVGLLTTAILWINQFPDEIADRKSGK
                     HNLVVVLGIERARWGYLTLLVAAFGISIICTLAGLFPIGTLAMLGGVPLAVSASRILF
                     QHYKDRELVSANAKTINLQLIAGLLTAAGILISPLIG"
BASE COUNT          569 a          511 c          540 g          625 t
ORIGIN      
        1 gtccgcccga tggtccacca gtggttcgag gcgcgttaca gcccttcgat agcgattgtc
       61 ttgatcagcc tggcgttgct ggtcataaag ctcaccccgc gaacaacgct ccatccgctg
      121 gcgcgtattc tcctttgcac tggagcaggt tttttggggt ttgggttttt ccgggtgacc
      181 ctgggcttgg tctatgctga ggcgctggtc tgggctaact tctgggaaga gcttaccgag
      241 ctgatgttcg tcgttgctgt gatatacacc ctgtgggttt tccggcgtac gttgttaccg
      301 gacaacaaat ttctggagac acacacttcg ttataatagt tatctttatt actaaccttg
      361 cactttaaga acttctgtag cgcctcagag cttgtgagac aaaattgggg caaaaactaa
      421 gagacacttt taggatgcaa cacccacaca aatatacata ctgctacgct tgaaagtatt
      481 cagcctctac gtgcagatat gcacgatgaa ccataatggt aaaatgccct tatccaaaac
      541 cagcacttcc tttagggagt agaataggga gtagaagccc tccctctttt ttccattgaa
      601 gattggttca accaacttca ggaaatttta agaaacactc gcaaaaatta tgatttaata
      661 tatacaaatt tacccatctt aatcttattg gagaacgtca atgaaacaca cagaagttaa
      721 aagcacaatc acttgcgata tggaaggccg tattgaaacg tataacaagg gcgctgaaga
      781 aatatttggc tacagtgccg aagaagtaat aggccataag cgagtatcac tattctcacc
      841 tggactgatc gtcttggaac atgtaccaac ctggctgaag actgccagcg agactggaga
      901 atataaaggc caaaccgtgt ttgttcgaaa agacggttca actttcaacg ctgaaattcg
      961 cattacaccc acttttcgcg atggtaaaca ggtcggatac tgcggggtca ccaaacctct
     1021 ggatccatcc cagagggcaa atgcaatgcc gaaaatcagt atgttgacca aagttttcgc
     1081 tggcatggta atcacgcgaa tgccatttct gacagccagc cttatcccag tcttaatcgg
     1141 tgcaacctgg gcagtggttg cgcgctcggc atcgcctttc ccctggttgg tattcatcct
     1201 tacattgctt ggtgctgcag ccctgcatgt tgctgctaat acattcaacg attactttga
     1261 ttggacaagt ggcacagata aagtcaataa tgactacttt ttacctttct ctggaggaag
     1321 tcgctcaatg gagcttggct taatttctga aaaaggtgtg ttgaatgtgg gcctggttgc
     1381 tttaggtttg gcctttgggt ttggcttgac cgtgatgttc ttgagccaac aaccattgct
     1441 aatcctgttt ggacttgcag gggcattctc agcttatttc tacacggcac caccattgcg
     1501 gctggtcgct cgcaaggggt tgggagaatt attaattggc ttgaattttg gtccgttgat
     1561 gactgctggc accgtgctag cactgacagg tcgcgtggcg tgggaagatt ttgtaattgg
     1621 tttaccagtt gggttgctaa ctacagccat tctatggatc aatcaatttc cggatgagat
     1681 tgccgatcgc aaaagtggta aacacaatct agtggtagtg ctgggtatag aacgtgcccg
     1741 ctggggctat ttaaccctgt tggttgctgc ttttggcatt agtataattt gtacactggc
     1801 aggactattt ccaatcggca cgctggcgat gcttggggga gttccgcttg cagtctcagc
     1861 cagccgtatc ctcttccagc attacaagga tcgagaactt gtatctgcca atgccaaaac
     1921 aattaacctt caattgatcg cggggttgct taccgcagcg ggcattttga tcagccctct
     1981 gataggatag cagcgaggtc attctaaatg atgttgggac aagcgacacc ggttgcggat
     2041 aaatcgcgca tgttagatag attacccttt caccattttc gatcgttatt ccacgaccag
     2101 cagcaagtct agaattcggc gtttcccgtt agatggtgtt tcacgatctc caaaaccagg
     2161 gataaactca ggaaattgta ttcggtgaga gaagagtaat agtgaccaag gctccccaac
     2221 attccggtta agcacacggc atgct
//
LOCUS       DBNHOH010000366         5952 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_790356, whole genome shotgun sequence.
ACCESSION   DBNHOH010000366 DBNHOH010000000
VERSION     DBNHOH010000366.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5952)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5952
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_790356"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="AC16V7_06525"
     CDS             <1..300
                     /locus_tag="AC16V7_06525"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR6256810.1"
                     /translation="NDPCFELGNTCQELVYDETQIRQLCAAYFGEATPARLARMKLNM
                     IMSDVGWALWAAIQAKISTIEFDFWGWAAERWGRAEAKMDSPEFPAWLDFVQKAP"
     gene            368..1306
                     /locus_tag="AC16V7_06530"
     CDS             368..1306
                     /locus_tag="AC16V7_06530"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR6256811.1"
                     /translation="MKLYYATDIHGSEICWKKFISAGKFYEVDTLILGGDMTGKAIVP
                     IIAQGNDRYKVTLLEQETILDGREEVEKVVKTIHNRGYYPYVTDPEEVAEISSTPGRS
                     DELFLSQALETVERWMAYAEEKLSGTGIRCYVCPGNDDMMEIDAVIAASKSVQLAEGR
                     VVRLDDHHEMASTGWSNPTPWDTHREEPDEALLERIEAVISQVQDIPNAVFNLHPPPY
                     GSGLDEAPELTDDLRPAYAGRSLIPVGSRAVLKVIEKYQPLVSLHGHIHEGKGTRKLR
                     RTLCINPGSMYEQGILHGVVMELKPKGLGNYLMVTG"
     gene            1482..3341
                     /locus_tag="AC16V7_06535"
     CDS             1482..3341
                     /locus_tag="AC16V7_06535"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIR6256812.1"
                     /translation="MSEVTLFVRKASGLVRSWSIFDAFIYATFSINLITLGLFIFSYC
                     YYFEGNLATAVVVGGAFTIFEVIVYASLISAMPRAGGDYVWQSRILGRGWGFILAVTG
                     WWFILWLWVPLYGQMLNYEFFTPILGVLGAKDAALWFTNTPEGLLTGMLLVCALVFVY
                     IGIGMKWYARVQKFCFWGGMVGLLLVFILLLFGNNATFVNNLNTMMPKLFGTEPGVDL
                     YQATLDAGAEAGSVVLPLGNIAFLASFAVIPMIVFFNLWPNWGSTLYGEVRGASDYKR
                     NFWGMAAAVVVTAALALILFALISKTIGWNFYNSANGAFWNYTWGYTDQPPPLPIWPY
                     PVLFAAMLTTSPVLQFIVVLLVSLWWFGWSGTVFLSSTRVIFAAAFDRMLPEWASAVE
                     PRTRTPVNALLLMVIPSVVISILYAYDIFSLQTIVLDATLVIAVTFLGTTVAAILLPW
                     RQKEVFDGSPIAKYQVPSWLSWIVTLLYALAAIYLIRRTYTVGLEVFGGFADLGALTR
                     LMVWLIAILSVVNAALLVWIFYFAIRKLLSGETFPLITVAGLIFFGFLDWLLVEWFWD
                     PGYLYGIGWQNDISMLFMVAMYGLAAAIFYGFNAYRRRQGIDVDKVYKEIPVE"
     gene            3622..4782
                     /locus_tag="AC16V7_06540"
     CDS             3622..4782
                     /locus_tag="AC16V7_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6256813.1"
                     /translation="MPQKTSISRRDFLKMSALTGGAAFLAACGGGPAAPSGANLPDLT
                     SGNSIPLEELIAAAKTEGELTTIALPHDWANYGEIIETFKTKYGLKVNELDPNAGSAD
                     ELEAIRANKESKGPQAPDVIDVGVGHTETAMKDGLVAKYKVSTWDSIEMKDPDGYWWA
                     EYYGVLVFEVSKPAIENSPQDWEDLLKPEYKGKIAMAGDVLKSNQSVQTVLASGLSRA
                     GGDITKAAEAGLQFWKEVNDAGNFIPVIADQGTIAQGETPIVMEWDYLALANRDSLAG
                     NPDLEIVVPKTGVLAGPYAGGISAYAPHPYAARLWWEFVMSDEGQNLYLKGYAHPIRF
                     NDMAKRGVIPQEMLDKLPPAAAYEKAFFPTIQQLTETKTYITENWRKVVYGE"
     gene            5040..5945
                     /locus_tag="AC16V7_06545"
     CDS             5040..5945
                     /locus_tag="AC16V7_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006469433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6256814.1"
                     /translation="MSVARGQPSSGSKAGGGPSKRFSWDWVGLAPFFAFAIMFLFLPS
                     VSIFIRSFEAPGGGFTISNLVALVTQRDLINAYKTSLSISLITATGGAIFGFLLAYAI
                     VLGGLPPALRTFMTTFSGVASNFAGVPLAFAFIATMGNAGLVTRWLESALGLDIHARG
                     FTIYSIWGLSIVYMYFQFPLMVLIMAPALDGLRREWKEACQNLGGTNWHYWRHVAFPI
                     LLPSLLGTTILLFGNAFGAYATAYAFSGSFINLVTIVIGAQIQGDVLYNPGLGNALAL
                     GMIIIMSLSMSAYIYLQRLSARWVR"
BASE COUNT         1231 a         1692 c         1657 g         1372 t
ORIGIN      
        1 aatgatccct gctttgagct gggcaatacc tgccaggagc tggtctacga tgaaactcaa
       61 atccggcagc tatgcgcggc gtacttcggt gaagccaccc ccgccaggct ggcgcgcatg
      121 aaattgaata tgatcatgtc cgatgtgggc tgggcgctgt gggcagccat ccaggccaag
      181 atctccacca ttgaattcga tttctggggc tgggctgccg agcgatgggg gcgtgccgaa
      241 gccaagatgg actcgccgga gttccccgcc tggctggact tcgtccaaaa ggctccctga
      301 ttgccgatcg ttgactactg attgctgacc gctgattgct gactgttgat tgtaaaggag
      361 agaattaatg aagctctact acgccacaga tattcatggt tcggaaatct gctggaaaaa
      421 gtttatcagc gccggaaaat tctacgaggt ggacaccctg atcctgggtg gagatatgac
      481 cggcaaggct atcgtgccca tcatcgccca gggcaatgat agatacaagg tcaccctctt
      541 ggaacaagag accatcctgg acgggcgcga agaagtggag aaggtggtca agaccatcca
      601 taatcgaggt tactatcctt acgtgacgga cccggaagaa gtggcagaaa tatcgtctac
      661 cccaggcagg tcggatgagc tcttcctgtc gcaagcactt gagaccgtgg agcgctggat
      721 ggcctacgcc gaggagaaac tgtcaggcac cggaatccgc tgctacgtct gcccgggcaa
      781 tgacgacatg atggagattg atgcagtcat cgccgcctcc aagagcgtgc aattggccga
      841 gggccgggtg gtcagattag acgaccatca cgagatggcc agcactggtt ggagcaaccc
      901 caccccctgg gacacccacc gcgaagaacc cgacgaagcc ctcctcgaac gtatcgaggc
      961 ggtcatatcc caggtgcagg acattcccaa cgccgtgttc aacctgcacc cgccgccata
     1021 cggcagcggg ctggatgaag cgcctgagct gaccgatgat ctgcgccctg cctatgccgg
     1081 ccgctctttg atccccgtgg gcagccgcgc cgtcctcaaa gtgatcgaaa aataccagcc
     1141 tctagtgagc ctgcacgggc atatccatga aggcaagggc acgcgcaagt tacgcaggac
     1201 gctgtgcatc aaccctggca gcatgtacga gcaaggcatc ttgcatggtg tggttatgga
     1261 gctgaaacct aaaggcttgg gcaattacct gatggtgaca ggttgagctc ctcacccctt
     1321 cccggttccc ctacccttat tccaagggag ataggggaaa aggttggggt gagggagtta
     1381 gaggaaggag gtggacctga gtagattgga acacataagc aaaagtaccc ggttttcaat
     1441 ccattcatca ttagatatcc caaaaatgga ggagtactga catgagtgag gtaacacttt
     1501 ttgttcgcaa agcaagcggc ctggtgcggt cctggtcgat ctttgacgcg tttatctacg
     1561 caaccttctc gatcaacctg attacgctag gtctgtttat cttctcctat tgctactatt
     1621 ttgaaggcaa cctagcaacc gcggtggtcg tcggcggggc tttcaccatc ttcgaggtga
     1681 tcgtctacgc cagcctgatc tctgccatgc cgcgcgccgg tggcgattac gtctggcaat
     1741 cgcgcatctt ggggcgaggc tggggcttca tcctggccgt caccgggtgg tggttcatct
     1801 tgtggctgtg ggtgccgctc tacgggcaga tgctcaacta cgagttcttc acccccatct
     1861 tgggcgttct gggcgccaag gatgccgcct tgtggttcac caatacccct gaaggtctgc
     1921 tcaccggcat gctactggtg tgcgccctgg tcttcgtcta catcggaatt ggcatgaagt
     1981 ggtatgcccg cgtgcagaaa ttctgtttct ggggtggcat ggtcggcctg ctgctagtct
     2041 tcatcctgct actcttcgga aacaacgcca ccttcgtcaa caatctcaac accatgatgc
     2101 ccaaattgtt cggcaccgaa ccaggtgtcg atttgtatca agccacattg gacgcgggtg
     2161 cggaagcggg ctcggttgtc ttgcctctag gcaatatcgc cttcctggcc agcttcgccg
     2221 tgatccccat gatcgtgttc ttcaacctgt ggccgaactg gggctccacg ctgtacggcg
     2281 aggtgcgcgg cgcttcggac tacaaacgca acttctgggg catggctgcc gccgtcgtcg
     2341 tgactgctgc cctggcgtta atcttattcg ccctgatcag caagactatc ggttggaact
     2401 tctacaacag cgccaatggc gctttctgga actacacctg gggctacacc gatcaaccac
     2461 cgccacttcc tatctggccc tacccggtgc tcttcgccgc tatgctgacc accagcccag
     2521 tgctgcagtt catcgtggtg ctgctggtga gcctgtggtg gttcggctgg tcgggtacgg
     2581 tcttcctgag ctctacgcgc gtcatcttcg ccgcagcttt cgatcgcatg ctgccagagt
     2641 gggcctcggc tgtcgagccg cgcacgcgca cgccagtgaa cgccctgctc ctgatggtca
     2701 tcccatcggt ggttatctcg atcttgtacg cctatgatat cttcagcctg caaaccattg
     2761 tgttggatgc caccctggtc atcgccgtga ccttcctggg caccaccgtg gcagccatcc
     2821 tcctgccctg gcggcagaaa gaggtcttcg atgggtcacc gatcgccaag taccaggtgc
     2881 caagctggct gagctggatt gtaacgctgc tgtatgcgct tgccgcaatc taccttatcc
     2941 gccgcaccta tacagtagga ttggaggtat ttggcgggtt tgccgatctg ggcgccttga
     3001 ccaggctgat ggtatggctg atcgccatct tgagtgtggt caacgcagcg ctgctggtgt
     3061 ggattttcta ctttgccatc cgtaagctac tgagcggcga gacgttcccg ctcattacgg
     3121 ttgccggttt gatctttttc ggcttcctgg actggctgct ggtcgagtgg ttctgggacc
     3181 ccggctacct gtatggcatc ggttggcaga atgacatctc gatgctcttt atggtggcga
     3241 tgtacggtct ggccgcggcc atcttctacg gcttcaacgc ctaccgccgc cgccagggca
     3301 ttgacgtgga caaggtctat aaagaaattc cggttgaata ggtctccatg tgcgacgcac
     3361 caataaggtg cgtcgcacat tacttccgat tattaaaaac tgctttgact ttgctggtta
     3421 atgatgctac aatttacccc tgcgcgcttt ttaccatgcg cgcttcccat tacagctaca
     3481 tgtcgcttcc atttgtgtaa tgtcgggggc gaatattagc catacaggag gtgaaccgcg
     3541 caaacacttc tgagttaaaa tgcactttca accaccaatc tttattgaat tactcatact
     3601 aaacaaactt cggaggacaa gatgccacaa aaaacatcca tcagccgccg cgatttcctg
     3661 aaaatgagcg ctctcaccgg cggagccgcc ttcctagcgg cttgcggagg tggtcctgct
     3721 gctcctagtg gggccaatct gcccgacctg acttctggta actccatccc cttggaggag
     3781 ctgattgccg cggcgaagac agaaggcgaa ctgaccacca tcgcccttcc gcacgactgg
     3841 gcaaactacg gtgagatcat cgagaccttt aaaaccaagt atggattgaa agtcaatgaa
     3901 ttggacccga acgccggctc agctgacgag ctggaagcca tccgggccaa taaagagagc
     3961 aaaggcccgc aagcccccga cgtgatcgac gtcggcgttg gccataccga aacggccatg
     4021 aaggacgggc tggtggctaa gtacaaagtg tctacctggg atagcatcga gatgaaggac
     4081 ccggatggct actggtgggc tgagtattac ggtgtgctgg tttttgaggt gtccaagcca
     4141 gctatcgaga acagccccca ggactgggaa gatctgctca agccagagta caaaggcaag
     4201 atagccatgg ctggcgatgt gctcaagtcc aaccagtctg tgcaaaccgt gctggcgtct
     4261 ggcttgtctc gcgccggtgg tgatatcacc aaggcggcag aagcaggtct gcagttctgg
     4321 aaggaagtca acgacgctgg caacttcatc cccgtcattg cagaccaggg cacgattgcc
     4381 cagggagaga cgccgattgt gatggaatgg gactacctgg ccctggccaa ccgagactcc
     4441 ctggcgggca accctgatct ggagattgta gttcccaaga cgggtgtgct cgccggcccg
     4501 tacgccggcg gcatcagcgc ctacgccccg cacccctacg ctgcccgcct gtggtgggag
     4561 ttcgtcatga gcgacgaagg ccagaacctg tacctgaagg gttacgccca ccccatccgc
     4621 ttcaacgata tggccaagcg cggcgtgatc ccgcaagaga tgctggacaa gctgccgcca
     4681 gccgcagctt acgaaaaagc gttcttcccg accatccaac aactgaccga gaccaagact
     4741 tatatcactg agaattggcg caaggtcgtt tatggcgagt agaaatttcg gtcgttgatc
     4801 aacccgcagt tctactgcgc ttgaatacaa aatgttcatt gcgagactgc ctgcttgacc
     4861 tgtctccagc gggcagtctc caaagaaact gtcactgcga gcctgcgagc ctgccgtttt
     4921 cacctaacct gtaggtctgg acaggtcggc aggcgaagca gtctcacccg tcgtcaagtc
     4981 gagattgctt cagtcgtccg aatggctcct tcgcaatgac ttgtcaggag gtgctgacca
     5041 tgagtgtagc ccgaggacaa ccatcatccg gctccaaagc cgggggaggt ccatccaaac
     5101 gtttttcctg ggattgggtg gggcttgccc cgttttttgc ttttgccatc atgttcttgt
     5161 ttcttccgtc ggtttcgatc ttcatccgca gcttcgaagc gcctggtggt ggcttcacta
     5221 tcagtaattt ggttgccttg gtgacccaaa gggatctcat caacgcctac aaaaccagct
     5281 tgagcatcag cctgatcaca gccacggggg gtgctatctt cggcttcctg ttggcttatg
     5341 cgattgttct aggagggctg ccgccggctc tacgcacttt catgaccacc ttttcgggtg
     5401 tagcctccaa ttttgccggc gtgccgctag catttgcctt tattgccacc atgggcaatg
     5461 ccgggctggt cacccgctgg cttgagtctg ctctgggact ggacattcac gcccgaggct
     5521 tcaccatcta ctcgatatgg ggcttaagca tcgtctatat gtacttccag ttcccactga
     5581 tggtgctgat catggccccc gccctggatg gcctgcggcg cgagtggaaa gaagcttgcc
     5641 aaaacctggg cggcacaaac tggcactact ggcgccatgt agccttcccc atcctgctgc
     5701 cgtccctact gggcaccacc attctgttat tcggcaatgc ctttggtgcc tacgccactg
     5761 cttacgcctt ctcgggcagc ttcatcaacc tggtgaccat cgtgatcggc gcccaaatcc
     5821 agggagatgt gctgtataac cccggcttgg gcaatgcact ggcgctgggc atgatcatta
     5881 tcatgtcgct gtcaatgtct gcatatatct atctgcagcg cctgtcagcg cgatgggtgc
     5941 gatgagagat ga
//
LOCUS       DBNHOH010000367         5028 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1110960, whole genome shotgun sequence.
ACCESSION   DBNHOH010000367 DBNHOH010000000
VERSION     DBNHOH010000367.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5028)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5028)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5028
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1110960"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="AC16V7_06550"
     CDS             complement(<1..242)
                     /locus_tag="AC16V7_06550"
                     /inference="COORDINATES: protein motif:HMM:NF026248.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4491 family protein"
                     /protein_id="HIR6256815.1"
                     /translation="MNLTGFYLGLLMLCAIGFGFLWVIKLEYYLGARVWKAVLGVGVL
                     ICLASLFTPSFTLSAILGILGGSVVWGATELSPQEER"
     gene            complement(310..981)
                     /locus_tag="AC16V7_06555"
     CDS             complement(310..981)
                     /locus_tag="AC16V7_06555"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR6256816.1"
                     /translation="MDEDVFYTDERQRMVEEQLIYRNIRDERVLEAMRSVPRHAFVPL
                     EYRHMAYSDGPLPIGSGQTISQPYIVALMTQLLRLKGDENVLEVGTGSGYQAAVLGKL
                     AKQVYTIERYTELADQAAAVLQKLGLNNVRVHIGDGSLGLPEHAPFQAILVTAAAPKV
                     PQSLLEQLDEGGRLVVPVGGRMNQFLELWERRGAKFAQDVLVPVAFVPLRGRHGWKED
                     SWEVF"
     gene            complement(988..1989)
                     /locus_tag="AC16V7_06560"
     CDS             complement(988..1989)
                     /locus_tag="AC16V7_06560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR6256817.1"
                     /translation="MMNIDASLPPVPLTSIPAIAQAAEQLGFAAVWSTETQHDPFLPG
                     ALIAEHTQRLQFGTAVAIAFARSPATIAYTAWDLAQASQGRFILGLGTQVKAHIERRF
                     GMPWPDSVVGKLREQITAVRAFWHTWQSGERLNHRGEYYKLTLMSPFFNPGPIEHPHI
                     PIYIAGVNTGLARLAGEIADGFHVHPLHTPRYLREVLLPAIEQGATGAGRSRQDLKIA
                     ASPFVVTGDQEDLFVRSQIAFYASTPSYKPVMALHSWQEVAERLSGLAARGEWGEMPA
                     LISDEMLSTFAVVAPTEDVRAALEERYTGLADRLTPYMPFIPGKRDAFWGALLKQAG"
     gene            complement(2014..3072)
                     /locus_tag="AC16V7_06565"
     CDS             complement(2014..3072)
                     /locus_tag="AC16V7_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIR6256818.1"
                     /translation="MNQLPPIDSDYLLTFLADLLNTPSPTGYAQRAIDLTQTAPEKFP
                     GLTLWRTRKGALVAEWRGAHSDAPRALTAHTDTLGAMVKEIKSNGRLRLSKIGGFAWN
                     TVEGEGCIVFTSRGETVRGALLLDKASSHVHGKEVNELPRNDQNMEVRLDTRTTNAEE
                     TQALGIQVGDFVAFDPRVEITNGFVRSRHLDDKACVACILAAVKSLYDAGLQPDQTTF
                     LLISNYEEVGHGAAAGIPPQVTELLTVDMAALGKGQTSDEFHATLCVKDSGGPYHHGL
                     SQRLRELADAHGLPYKVDIYPYYGSDGEAFWRAGGDVAVSLIGPGVDASHNYERTHVE
                     ALIATTQWIAAYLLDNRD"
     gene            complement(3069..3503)
                     /locus_tag="AC16V7_06570"
     CDS             complement(3069..3503)
                     /locus_tag="AC16V7_06570"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR6256819.1"
                     /translation="MPEFNYYHPIEVRYGDLDPQGHLNNAKYLTYMEQARVSYVKHLG
                     LWNSTSFLDVGIILADIHITFRAAVQFGQPVRVGVRVSRLGNKSMDMHYIIEDTRSGE
                     ELASGSSVVVTYDYHKNLAIPIPDEWRRVISAFDKIQARYKA"
     gene            3592..4890
                     /locus_tag="AC16V7_06575"
     CDS             3592..4890
                     /locus_tag="AC16V7_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6256820.1"
                     /translation="MENSDQDETQPFPVNPQDETNFDISDTQPTPVPRPNSAPPEADQ
                     TMPVNVDAAPTDFQGGDATMPVEVRSQPPQVLPPGPLAPKPKKRRFPALASWSLLGLL
                     GLLLIGLISAFGGYNAGIDQRKQAQASQEVAIADEQYQLALQDMQAGRYDLARQRFEY
                     VIELNPGYPGVTDKLADVLLYMNATATPTVTPTPTITPTPDTRNVQELFSQAQQELAN
                     SNWSTAIDTLLSLRKADPNYQPVWVDGMLYVSFLNRGREKILNQADLEGGIYDMTVAE
                     KFGLLDTESRGLQNWARLYITGASFWELDWAQVVYYFAQIAPALPNLRDGSGWTAAER
                     YRLALIGYGGDLAEKAEWCDAQEQLELALSMGPDEQASRLLADVSNVCSGGSSGGSDN
                     SNSNDNDNSQQPAETEAPPTEEQPGEPAATEPPPDSTPAP"
BASE COUNT         1076 a         1484 c         1404 g         1064 t
ORIGIN      
        1 cgctcttctt gaggggatag ttcggtcgcc ccccacacca cggatccacc caaaatgccc
       61 aggatggcgg atagtgtgaa cgacggcgtg aacagcgaag ccaggcagat cagcacaccg
      121 acgcccagca cagccttcca cactcgcgcc ccaagatagt actcgagctt tatcacccac
      181 aagaaaccga agccaatggc acagagcatc aacaaaccca agtaaaagcc agtcaaattc
      241 acgtcacaac ctcatatctt gcccagacta tgcaaatccc aaagagctca gattctttgt
      301 cgccccttgt taaaaaactt cccaggaatc ttccttccat ccgtgtcgcc cgcgcagggg
      361 cacgaaagcc actggtacca gcacatcctg ggcgaacttc gctccgcgtc gctcccatag
      421 ctccaggaac tggttcatcc gaccgccaac cggcacaacc aaccgcccac cctcatcgag
      481 ctgctcaagt aaggactggg ggactttagg cgcggctgca gtgacgagaa tggcctggaa
      541 gggggcgtgc tcaggcaatc ccaaggagcc atcaccgata tgcacgcgta cattgttcag
      601 ccccagtttc tgcaacacgg ccgcggcttg gtctgctagc tcagtgtagc gctcaatggt
      661 gtacacctgc ttggccagct tgcccagcac tgctgcctgg tatcctgagc ctgtgcccac
      721 ttccagcacg ttctcatctc ctttcaagcg cagcaattgg gtcatcaagg cgacaatata
      781 gggttgggag atcgtctgcc cgctgccgat gggcaacgga ccgtcgctgt aggccatgtg
      841 gcgatactct aaggggacaa aggcgtggcg gggtacagat cgcatagctt ccaggacacg
      901 ctcatcgcga atgttgcggt agatgagctg ctcctctacc atgcgctggc gctcgtcggt
      961 gtaaaaaacg tcctcatcca tggctgctca gccagcttgt ttcaaaaggg ctccccaaaa
     1021 tgcatctcgt ttacctggga taaaaggcat ataaggtgtc agccggtctg ccagaccggt
     1081 gtatcgctcc tccaatgccg cccgcacgtc ctctgttggg gcgaccacag cgaaggtaga
     1141 tagcatctcg tcgctgatca gagctggcat ctcaccccac tcgccgcgtg ccgcgagccc
     1201 agagaggcgc tcggcgacct cctgccagct gtgcagcgcc atcactggct tgtaagacgg
     1261 cgtggaagcg tagaaggcga tctgtgagcg cacgaaaagg tcttcctggt ctccagtgac
     1321 cacaaagggc gaggcggcga tctttagatc ttgtctggaa cgacctgccc cggttgcccc
     1381 ctgctctatg gcgggcagca gcacctccct caggtagcgg ggtgtatgca acgggtgtac
     1441 gtggaagccg tcggctatct cacctgccaa tctagccagc ccggtattca ccccggcgat
     1501 gtagatgggg atgtgcgggt gctcgattgg gccggggttg aagaatggtg acatcagcgt
     1561 gagcttgtaa tattctccgc ggtggttgag ccgttcgccg ctttgccagg tatgccagaa
     1621 ggcgcgtacg gcggtgatct gctcgcgcag cttgcccacc acggagtcgg gccagggcat
     1681 cccgaaacgt cgctcgatgt gtgcttttac ctgtgtgccc aagcccagga taaagcgacc
     1741 ctgggaggct tgtgccagat cccaggcagt ataagcgatg gttgccgggc tgcgcgcaaa
     1801 ggcgattgcc acggctgtgc cgaattgcag gcgctgggta tgctcggcga tcagcgcccc
     1861 cggcaggaag ggatcgtgct gtgtctctgt agaccacacc gccgcaaagc ccaactgctc
     1921 tgcagcctgg gcgatagcag gtattgaggt taatggaacc gggggaaggg atgcgtcaat
     1981 attcatcatt caatttgtac tactcttatt catctagtct ctgttgtcta acagataagc
     2041 agcaatccat tgtgtggtag caatcagcgc ctccacatgc gtgcgctcgt aattgtgcga
     2101 ggcgtccacg cccggaccaa tcaacgacac ggccacatca ccgccagcgc gccagaaagc
     2161 ttcgccatct gagccgtaat atggatagat atcgactttg tatggcaggc cgtgagcgtc
     2221 tgccagctca cgcaggcgct ggctcaggcc gtggtggtac ggtccgcccg agtcctttac
     2281 acacagcgtg gcgtggaact catcagaggt ctgcccttta cccaaagcgg ccatatccac
     2341 agtcagcagc tcggtcacct gtggtggtat gcctgctgca gcgccatgcc caacttcctc
     2401 atagttgctg atcagaagga atgtggtctg gtctggctgc aagccagcgt cataaagcga
     2461 cttgaccgcc gccaaaatgc aggccacaca ggctttgtcg tccaggtggc gagagcgcac
     2521 aaaaccattg gttatctcga cgcgaggatc gaaggctacg aagtcgccta cctgaatgcc
     2581 tagagcttgt gtctcctcag cgttcgtggt acgggtatcc aggcggactt ccatattctg
     2641 gtcattgcgg ggcagctcat tgacctcttt gccatgcaca tggctggagg ccttgtcgag
     2701 caacaatgcg ccgcgcacgg tttctccccg gctggtaaat actatgcatc cttcgccttc
     2761 caccgtattc caggcgaacc cgccgatctt gctcagcctg agccgcccgt tgctcttgat
     2821 ctctttgacc atcgcaccca gggtgtcagt gtgcgcggtc agggcgcggg gtgcatcgga
     2881 gtgggcgccg cgccactcag ccaccaatgc ccctttgcgt gtgcgccaca gcgttagccc
     2941 agggaacttc tctggcgctg tttgggtcag gtcaatcgcc cgctgggcgt agcccgtggg
     3001 gctgggtgtg ttcagcaggt cggcgagaaa cgtaaggaga taatcgctgt caatgggggg
     3061 taattggttc atgccttgta ccttgcttga attttgtcaa aagcgctgat caccctcctc
     3121 cattcatccg gaatagggat cgccaagttt ttgtgataat cgtacgtcac cacaaccgat
     3181 gagccgcttg ccagctcctc gccactcctg gtgtcctcga tgatgtaatg catgtccatg
     3241 cttttattgc ccagccttga gacgcgcacc cccacgcgca ctggctgccc gaactgcacc
     3301 gcggcacgga aggtgatgtg gatgtccgcc aggatgatgc ccacatctaa aaaggatgtg
     3361 ctgttccaca agcctagatg cttgacgtag ctgacgcggg cttgttccat gtaggttagg
     3421 tatttggcgt tgttgagatg tccctggggg tctagatcgc cgtaacgaac ttcgatggga
     3481 tgatagtaat tgaactcggg cataatggga ttatatacta aatcgtgggg agtaatttaa
     3541 cccatttagc tatcccgact caagccccac gtctgcggta taatgtgccg catggaaaat
     3601 tctgatcaag acgaaactca accttttcct gtgaatccgc aggacgagac aaattttgat
     3661 ataagcgata cacaaccaac gccagtaccc cgcccaaaca gcgccccgcc cgaggcggat
     3721 cagacgatgc cggttaacgt cgatgcagcg cccaccgatt ttcagggcgg agacgcaacc
     3781 atgccagtcg aggtccgaag ccaaccgcca caggtactcc cgccgggccc cctggctcca
     3841 aaacccaaga agcgccgctt tccagctttg gcgagctggt cgctcctggg cttgctgggg
     3901 ctgttactga ttggcttgat aagcgccttt ggcggttaca acgcaggcat cgaccagcgc
     3961 aaacaggccc aagcctcaca ggaggttgca attgccgacg agcagtacca gttggctttg
     4021 caggatatgc aagctggccg gtacgacctg gctcgccagc gcttcgagta tgtgattgag
     4081 cttaacccgg gctatccagg cgtcacggat aaactggcgg atgtgttgct ctacatgaac
     4141 gctaccgcta cgcctactgt tacccccact cccaccatca ctcccacgcc cgatacacgc
     4201 aacgtccagg agttgttttc acaggcgcag caggaactgg ctaattccaa ttggagtact
     4261 gccatcgaca ccctgctatc cctgcgcaaa gccgatccga actaccagcc cgtttgggtg
     4321 gacggtatgc tgtatgtctc tttcctcaac cggggacggg aaaaaatcct aaaccaggct
     4381 gatctggaag gcggtatcta tgatatgacc gtcgccgaaa agtttggact gttggacacc
     4441 gaatcgcgcg gcctgcagaa ctgggcacgc ctgtatatca ctggagccag cttttgggag
     4501 ctggattggg cgcaggtagt ctattacttt gcccaaatcg ctcccgctct gcccaacctg
     4561 cgcgatggtt ctggctggac cgcagccgag cgctaccgtc tggccttgat cggctacggt
     4621 ggggacttgg ctgaaaaagc cgaatggtgt gatgctcaag agcagcttga actggcgctc
     4681 tctatgggac cagatgagca ggcttcgaga ttgctcgcag acgtgtcaaa tgtctgcagc
     4741 ggagggtcta gcggcggaag cgacaacagc aatagcaatg acaatgataa cagccaacaa
     4801 cctgctgaaa cagaagcacc tccaaccgaa gagcagccag gcgaaccagc tgctacagag
     4861 ccgcccccag acagcacacc tgcgccttag gtaatgagct cgccacccgt ttgggcgctt
     4921 agcctgatct attggctgca tatgctcgct accgtggttt ggattggcgg gctggcagcg
     4981 ctctcgttct tggtgctgcc ttctgccaag cggatactgg aaccggct
//
LOCUS       DBNHOH010000368         2397 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_21735, whole genome shotgun sequence.
ACCESSION   DBNHOH010000368 DBNHOH010000000
VERSION     DBNHOH010000368.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_21735"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(291..467)
                     /locus_tag="AC16V7_06580"
     CDS             complement(291..467)
                     /locus_tag="AC16V7_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256821.1"
                     /translation="MVALLKFIFGLIGAVIGLVVGLIGLVVGLVGGVIGITVGCLVLA
                     IVLAPLILLLVLIF"
     gene            complement(690..2048)
                     /locus_tag="AC16V7_06585"
     CDS             complement(690..2048)
                     /locus_tag="AC16V7_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase type-2 family protein"
                     /protein_id="HIR6256822.1"
                     /translation="MTFSLTQTEWGEKVDRILAASLGAVEPGSALRRFVQRNADQLII
                     DGQIYDLASYRRIWVVGAGKAGALMACAMADILDERLEGGLVIVKDGYLQPCEGLKPE
                     RGLRLVEAGHPLPDGRGVRATQEIISLLSDCNAEDLVICLISGGGSALLTAPAPGISL
                     HDLQTLTQLLLASGANINEINALRKHLDQVKGGQLARMAYPAHLATLIVSDVVGNPLD
                     VIASGPTVSDGTTFSDAYAVLERYNLVGQVPQAILAYLQSGCAGEILETPKEGDSIFT
                     AVQNVIVGSNMQAVAAALRQAQQEGFNTQLLTTYMQGEARQVGRLLAAIVRQVATTGQ
                     PISRPACVVVGGETTVTLQGDGLGGRNQELALGAVEDLGGLMDVALVTLATDGGDGPT
                     DAAGAVVTGETLQRARQLGLRPSEYLQRNDAYHFFEALGDLIITGPTGTNVNDLAFLF
                     AF"
     gene            complement(2045..>2397)
                     /locus_tag="AC16V7_06590"
     CDS             complement(2045..>2397)
                     /locus_tag="AC16V7_06590"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014626.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HIR6256823.1"
                     /translation="KKEGQGKIPTYIRLASGSPFAIAGLWESWNAPDGSNVLSCTIIT
                     TQPNEFMATIHNRMPVILPASSYSLWLESGEQDTRRLADLLAPYTSGEMQAYPVSPLV
                     NNPKHDVPELIQPR"
BASE COUNT          563 a          699 c          605 g          530 t
ORIGIN      
        1 ttttttgtat tattcggaaa aatcaaatgg ataatatttc caaatcagca taagccgagc
       61 catgagcact actgaacgta caaaaaattt atccctagcg tgcgcacaaa atcgcccgtc
      121 acacaccagc taacaggatg agttatccaa gcatgtcata gttctaaccc cctcttccta
      181 gttcagaggg ggttagaagt ttaggcttct atattgaaat agcggggaaa gtttagaaac
      241 tgagctctaa gtgggttgtg tcaagcttta gccatacagt atgataggca tcagaagatc
      301 agcacaagca acaggatgag tggcgcaagg acgatcgcca aaaccaggca cccgactgta
      361 atcccgatca ccccacctac caggccgacc actaaaccga tcagccccac caccaggcca
      421 ataacggctc caatcagccc gaagataaat ttcaatagtg ctaccatcgc aaggacctcc
      481 atttcctttc tattgatttc agtagttgag cagtctagga ggactttggc gggattaggc
      541 cagcgtgtct cgctgcgatt cccgcgcctt tcatctaact gcgcaaactt tgatttatat
      601 caatctacgc agttttgagc ttttagttgc aatcggagag caatttgccg tttccagtcc
      661 caagcattgc agatgtgtat tacaaatcct taaaacgcaa acaagaaagc cagatcgttc
      721 acattcgtcc cggtcggacc ggtgataatc aaatccccca gggcttcaaa gaaatgatag
      781 gcatcgttac gctgtaaata ctcagaagga cgcagaccaa gctggcgggc gcgctggagt
      841 gtctcccccg tgaccaccgc cccggctgcg tcagttgggc cgtcaccccc atcagttgcc
      901 aatgtaacca gggctacatc cattaagcca cccaggtctt ccacggctcc taatgccagc
      961 tcctggtttc gcccccccaa gccgtctcct tgcagggtga ccgtggtttc accacctacc
     1021 acgacacatg caggccgcga gatgggctgg cctgtcgtag caacctgccg aacgatcgcc
     1081 gccagcagcc tccccacctg tcgcgcttcc ccttgcatgt aggttgtcaa cagctgggtg
     1141 ttgaagccct cctgctgggc ctgcctgagg gcagccgcca cagcctgcat attgctccca
     1201 acgatcacat tttgaacagc ggtgaatata gaatctcctt ctttgggcgt ttccaggatt
     1261 tcccctgcgc acccactttg cagataggcc agtatggctt gcggcacctg gccgaccaga
     1321 ttatagcgtt ccagcactgc gtaggcatct gaaaaagtag tgccatcaga tacggttggg
     1381 ccggaggcga tcacatccag cgggttgccc accacatctg agacgatcaa ggtcgccaga
     1441 tgagccgggt acgccatgcg agccagctgc ccccctttga cctgatccaa atgcttgcgc
     1501 aaggcgttga tctcattgat attggcaccg ctggcgagca gtaattgggt gagggtttgc
     1561 aagtcatgta acgaaatgcc tggtgctggt gctgtgagca acgccgaccc gcccccagag
     1621 atcaggcaga tcaccaagtc ttcagcattg cagtcgctca gcagtgagat gatttcctgg
     1681 gtcgcgcgta ccccgcgtcc gtccggcagc ggatgcccag cctccacaag acgcaaacca
     1741 cgttcaggtt ttaatccttc gcaaggctgc aggtagccgt ctttgacaat caccaatcca
     1801 ccctccaggc gctcatccaa gatatcagcc atggcgcaag ccatcagcgc cccagccttg
     1861 cctgccccca cgacccaaat ccgacggtag gacgctaggt cgtaaatctg tccgtcgatg
     1921 ataagttggt ctgcgttgcg ctgcacaaag cgcctgagcg ccgagccggg ttccacagca
     1981 cccagcgagg cagccaggat gcgatcaacc ttttcgcccc actcggtttg tgtcaaagag
     2041 aatgtcatct cggctggatt aattcgggta cgtcatgttt tgggttgttc accaagggtg
     2101 aaaccggata agcctgcatc tcgcccgaag tatagggggc cagcagatcg gcaagccgac
     2161 gtgtgtcttg ctctccactt tcaagccaca gcgagtagga gctcgcgggg agtatgactg
     2221 gcatgcggtt gtggatggtc gccataaact cgttaggctg ggtggtgatg attgtgcagg
     2281 agagcacgtt cgacccatcg ggcgcgttcc atgactccca caatccagcg atggcaaatg
     2341 gactgcccga agccagtcga atgtaggtgg ggatcttgcc ctgcccctcc ttcttcc
//
LOCUS       DBNHOH010000369         1813 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1239958, whole genome shotgun sequence.
ACCESSION   DBNHOH010000369 DBNHOH010000000
VERSION     DBNHOH010000369.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1239958"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="AC16V7_06595"
     CDS             complement(<1..657)
                     /locus_tag="AC16V7_06595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6256824.1"
                     /translation="MIANLLQLTVRPVSDKDLQRLANLIHFEALVHRHLDWRAPLDWV
                     GYNPYLVAEHNDEILAALACPPDPPSVAWIRLFATSSEISVNLAWEELWNAACDQICN
                     LGDTALVAAIPLQNWFRKLLEKSEFVRSHNVIVLSWRRAPRPPASRALPGFIRTMELR
                     DINAVEELDKAAFGLIWQNSYSSLEVAFRQAAVATVLDNDGEIIGYQISTATPVGGHL
                     A"
     gene            complement(735..1286)
                     /locus_tag="AC16V7_06600"
     CDS             complement(735..1286)
                     /locus_tag="AC16V7_06600"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6256825.1"
                     /translation="MNEQISVNQLISQGKSAYQQGDFIAAARAFDAAGDSYSVSGDEL
                     NAAEMRNNSSVAYLQAGEAQAALVAVEGTPAIFATAGDLRRQGMALGNLAAALEAMNR
                     LEEATKAYLQSAELLESIGEKDLRAYVMKSLSALQLRSGRHIEALSTMQAGLDGIEHP
                     KPQQRLLKRLLNVPFKFLGKSSD"
     gene            complement(1295..>1813)
                     /locus_tag="AC16V7_06605"
     CDS             complement(1295..>1813)
                     /locus_tag="AC16V7_06605"
                     /inference="COORDINATES: protein motif:HMM:NF024949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6256826.1"
                     /translation="SLPKLEVLALYLGIPLDHFWGSKARSEDASPALDFDVKQLLRVR
                     QRMIGALVRQARTQGDISLEELSQEVGIAPDQLEACENGEAELPLPTLEAFCARLNRP
                     IEEFFDKQGPVGVWIGQQQAVQDFLQLPDEIQTFVAKPINRPYLELAQRLSEMSVDKL
                     RALGEGILEITL"
BASE COUNT          443 a          485 c          432 g          453 t
ORIGIN      
        1 ggccagatga cctcctacgg gggtcgcggt gcttatctgg tagccaatga tctctccgtc
       61 attatccaag acggtggcaa ccgcagcctg gcgaaacgcc acttctaaac tggaataaga
      121 attctgccat attaggccaa atgcagcctt atccaattcc tctacggcat tgatgtctct
      181 tagctccatt gtgcggatga acccagggag agctcttgat gctggaggtc ttggagcccg
      241 tctccagctc aacacaataa cgttgtgtga gcgcacaaat tcgctttttt cgagcaattt
      301 tctgaaccaa ttttgcagag ggatagcggc aactaacgct gtatcaccca ggttacatat
      361 ttggtcacag gctgcgttcc atagctcctc ccacgccaga ttgaccgaga tttccgatga
      421 tgtcgcgaat aaacgaatcc aggccacaga gggaggatct ggtgggcagg ccagtgccgc
      481 gaggatctca tcattgtgtt ctgcgaccag ataagggtta tagccgaccc aatccagcgg
      541 tgctcgccag tccaaatgcc ggtgaacaag cgcctcaaaa tggatcagat tcgccaatcg
      601 ctggagatcc ttatctgata caggccgaac agtaagctgc aggagattag cgatcatgaa
      661 cgccgtcttt atcgatgact attatatatg ctttgaaagc tggaatgaag cgattacaag
      721 aagcgggata acgtttagtc tgatgacttt cccagaaatt taaatgggac attcaataac
      781 cgcttcagga gacgttgctg gggctttggg tgttcgatcc catccagccc agcttgcatc
      841 gtagacaatg cctcgatatg gcgacccgag cgtaattgta atgctgagag tgacttcatt
      901 acataagccc gcaagtcttt ctccccaatt gattcgagca attcagcaga ctggaggtag
      961 gctttagtcg cttcctctag gcgattcatt gcctcaagag ctgccgccaa gttacccagc
     1021 gccatcccct gccggcgtaa atcgccagcg gtcgcaaaga ttgctggcgt cccttccact
     1081 gcaacgaggg cagcttgagc ttcgccagct tgcaaatagg caacactgct attattgcgc
     1141 atctcagcag catttagctc gtctccgcta acactgtagc tatctcctgc agcatcaaat
     1201 gcacgtgcag ctgcaatgaa atccccctgt tgataggcgg attttccttg actgattagc
     1261 tggttgacag aaatctgctc attcatactg tccctcataa tgtaatctcc aagatcccct
     1321 ctcctaaagc tcgcagttta tcgaccgaca tctcactcag acgctgtgcc aactccagat
     1381 atggacgatt gatcggctta gccacaaatg tttgtatctc atctggaagt tgaaggaagt
     1441 cttggacggc ttgctgttgc ccaatccaaa ccccaactgg cccttgttta tcaaaaaatt
     1501 cttcgattgg gcgatttaat cttgcacaaa acgcttcgag tgtcggtaga ggcagctcgg
     1561 cttcaccatt ctcgcaggct tcaagttgat caggggcaat gccgacttcc tgtgacaatt
     1621 cttccagcga gatatcgccc tgagttcgcg cttgacgcac cagcgcgcca atcatgcgct
     1681 ggcgtacgcg cagcagttgt ttgacatcaa aatccagtgc ggggcttgca tcttccgatc
     1741 tcgctttgct accccaaaag tgatccagag gtatacccag atacaatgcg agcacctcga
     1801 gcttgggaag cga
//
LOCUS       DBNHOH010000370         4303 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_85558, whole genome shotgun sequence.
ACCESSION   DBNHOH010000370 DBNHOH010000000
VERSION     DBNHOH010000370.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_85558"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="AC16V7_06610"
     CDS             <1..253
                     /locus_tag="AC16V7_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HIR6256827.1"
                     /translation="RFGYTVPTNVEAELMRMMIDRTKGPIYVVADHTKWGVVSNFEVA
                     PIHKDLKLITDEGLDEHARADLAARTVEVIIARAYPAKI"
     gene            297..1292
                     /locus_tag="AC16V7_06615"
     CDS             297..1292
                     /locus_tag="AC16V7_06615"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR6256828.1"
                     /translation="MSFFFKKSPKKTTRLFFATDIHGSERTFRKFINAGKFYQANVLV
                     MGGDITGKLLIPIIKEGNGHFRATLQGSIQKIETEAELQQLQERIGILGFYSQIMDED
                     EFRQTQVDPVAVDKLFRQLARQRLDDWVDLAETRLAGTGIKCFVTGGNDDDPDTLEPL
                     YRQGTQSFFGCENKVVYVDDEHSMVSMGYSTPTPWKTPREVSDDELGKLIEEMVAQVP
                     DKGKCIFNFHDPPLDSTLDTCPMLDWSKDPPEPIVKAGQIVLHGAGSQSVRNAIETHQ
                     PMLGLHGHIHESQSVAKIGRTTCVNPGSEYGEGVLRGCLVNFSNGVIEGYQMTSG"
     gene            1460..3313
                     /locus_tag="AC16V7_06620"
     CDS             1460..3313
                     /locus_tag="AC16V7_06620"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIR6256829.1"
                     /translation="MTATTAQAKPEVFTRKASGLVRVMSPYSAFVYNILTMGLIFPWT
                     YLWAPGALPGGKMAWGIILAMLIEIPIAFAYVWLSTALPRSGGDYVFESRVFGGGVAF
                     TVVMSGYVIWILQWVALSGWLLAYLGFAPLFLGLGATMGNASLSAIGLWFTTPSGIII
                     TSILNALVSMLILTSGFKNYVRFQWVMWYAVLLAFAVMFAVLFVTPASQFVERLNAFA
                     VASGAGENFYATATAAAQSAGVDLNPPFSLIATILVAPIAWTSLQWATYSAQQNGEIK
                     DARSFKNQAFIMIGSLIVTGLLLALLAVGLERLAGSEFLYVAGAGYWYALPEATVNGF
                     YLWPNIIAVAVTASPLIVLIIGLGYILNSHQIVHNCYIGMTRVLVAMSLDRLLPDWFS
                     KVDERRHTPVNAHLAYFLGSIPIILAYNLAPGWVGLTLGVTFGCGYVFIITCLAGALL
                     PYRAKEVYEASPGAKYNVSGYVGVLFTLLGVVAFIWTSWILAPDAFGSYPILVWLVRL
                     GSIVAVVLFLYPMRQYIMEWFSGKPMPWLSALGMLGGGFGMAMVVAFLLAPQLGILGN
                     WDFSNFPGGLWSQIIAFGIIVVSAVWYLLVKRAQSARGINVEYAFKEIPPE"
     gene            3495..>4303
                     /locus_tag="AC16V7_06625"
     CDS             3495..>4303
                     /locus_tag="AC16V7_06625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6256830.1"
                     /translation="MKNQARVVVIGGGIYGVNVAYHLAKLGWTDLVLVEKNEIASGES
                     AQAAGLVTQFATSQTMMQFRKYSIELYSELGLFDHVGSLRLASSKEQLHELQRSVSRA
                     KGIGMDVEIISPAEALKIMPQLSPKDLYGAIYLPRDGHLDPYTTTTNMARLVREMGCE
                     VVTNTRVTGIKLTPKGEVSEVVTTRGSIKCEHVINATGLWGPRLAAMAGLHLPTTPVD
                     HQHIALKAVPGNEFPHSTPCLRDPDNLVYMREEQGGLVIGGYEPNPKERWV"
BASE COUNT          903 a         1236 c         1198 g          966 t
ORIGIN      
        1 aaggttcggc tacaccgtgc caaccaacgt cgaagcagaa ttgatgcgca tgatgatcga
       61 tcgcacaaaa ggccccatct atgtggtcgc agaccacacc aaatggggcg tggtctccaa
      121 ttttgaggtt gccccaattc ataaagacct caagctaatc acagatgaag gtttagacga
      181 acatgctcgc gccgacctgg ctgcccgtac agtcgaggtc attattgcgc gcgcatatcc
      241 agcaaagatc tgacaggttg ctgcctgttt tatgatgaca atccggaggc atgaggatgt
      301 cttttttctt caagaaatcg cctaagaaaa ccacccgctt gtttttcgct accgatatac
      361 atggttcgga gcgtactttc agaaaattca tcaatgccgg aaagttttac caagcaaatg
      421 tgctggtcat gggcggagat atcacaggca aactgcttat ccccatcatc aaagagggca
      481 atggacattt tcgcgccacc ctacagggca gtatacagaa aatcgagacc gaggcagagc
      541 ttcaacagct tcaagaaaga attggaatac taggctttta cagccagatc atggacgaag
      601 acgaatttcg ccagacgcaa gtcgacccgg tggcggtgga taaactcttc cgccagctgg
      661 cgcgccagcg cctggatgac tgggtagacc tggccgagac acggctggcg ggcacaggca
      721 ttaagtgctt cgtaaccggc ggcaacgatg acgaccccga taccctggag ccgctttatc
      781 gccaggggac gcaatccttc ttcggctgtg agaacaaagt ggtctacgtg gatgacgagc
      841 acagcatggt cagcatgggc tacagcaccc caaccccgtg gaagacgccc cgcgaggtaa
      901 gcgatgatga gctgggcaag ttgatcgaag agatggtggc gcaggtgcca gataagggca
      961 aatgcatctt caattttcat gacccgccgc tcgactccac cctggacacc tgcccgatgc
     1021 tggattggag caaagacccc ccggagccca tcgttaaagc cggtcagatt gttttacacg
     1081 gtgcaggcag ccagtctgtg cgcaacgcca tcgaaactca ccagcccatg ctggggttgc
     1141 atggtcacat ccacgaatcg caatcggtgg ccaagatcgg gcgcacaacc tgtgtcaacc
     1201 ctggcagcga atatggcgaa ggggtgctgc gcggctgcct ggtgaatttt tccaacggcg
     1261 tgatcgaagg ctatcagatg acgagtggct gatactctgg agcctcccgc aggtttagaa
     1321 tctgcgggag gttttcacgg aaggaggtaa ctatcggagc agataagggt taactttaac
     1381 tagctattta gcctgatacg ttctgccaat cggcagtcgc aggccaatcc acatattctc
     1441 aaccaaagga ggatgtacta tgaccgcaac aaccgcccaa gccaagcccg aagtctttac
     1501 ccgtaaagcc tccggcctgg tgcgggtcat gtccccgtat tcagcatttg tttacaatat
     1561 cttgaccatg ggattgattt ttccctggac ctatttatgg gcacctgggg cgctgcccgg
     1621 cgggaagatg gcctggggga ttatcctggc tatgctgatc gagatcccca tcgccttcgc
     1681 ttatgtatgg ctgtccaccg ccctgccccg ttccggcggc gactacgtct tcgaaagccg
     1741 tgtcttcggc ggcggcgtgg ctttcacggt ggtgatgtcc ggctacgtca tctggattct
     1801 gcagtgggtg gcgctgtccg gctggctgct tgcgtacctg ggctttgcac cattgttcct
     1861 tggcctgggc gctacgatgg gtaacgcctc actatcagcc atcggcctct ggtttaccac
     1921 tcccagcggc atcatcatca ccagcatatt gaacgcattg gtctcgatgc tcatcctgac
     1981 cagtggcttc aagaactacg tgcgcttcca gtgggtcatg tggtacgccg tgttgctggc
     2041 attcgccgtg atgttcgctg tgctgttcgt taccccggcc tcgcagttcg tcgagcgcct
     2101 gaacgctttt gcggttgcat ccggtgctgg tgagaatttc tacgcaactg ccaccgcagc
     2161 ggctcagtct gctggagttg atttaaatcc ccccttcagc ttgatcgcca ccatacttgt
     2221 cgctcccatc gcctggacct ccctgcagtg ggctacctac tccgcccagc agaacggcga
     2281 gatcaaggat gcccgctcct tcaagaacca ggctttcatc atgatcggct cgctgatcgt
     2341 cactggcttg ctgctagctt tgctggcggt gggtctggag cgactggcag gttctgagtt
     2401 cctttatgta gctggtgcag gctactggta tgcgctgcct gaagcaacgg tgaacggttt
     2461 ttacctgtgg cctaatatca tcgccgtggc tgtgaccgcc agcccgttga tcgtgttgat
     2521 catcggtctg ggctacatcc tcaactctca ccagatcgtg cataactgct atattggcat
     2581 gacgcgcgtg ctggtggcga tgtcgctcga ccgcctgctg cctgactggt tcagcaaggt
     2641 agatgagcgc cgacacacac ccgtgaacgc ccacctggct tacttcttgg gcagcatacc
     2701 tatcatcctg gcgtacaact tggcaccggg atgggttgga ctgaccttag gcgtgacctt
     2761 cggctgcggt tacgtcttca tcatcacctg cctggccggc gcactgctgc cctaccgcgc
     2821 caaggaagtc tacgaggctt cccctggagc aaagtacaat gtcagcggtt acgtgggcgt
     2881 gctcttcacc ctgcttggcg tggtggcctt catctggacc tcctggatac tggctcccga
     2941 cgctttcggc agctatccta tcctggtctg gctggtgcgc ctgggttcca tcgtcgccgt
     3001 cgtcctgttc ctctacccga tgcgccagta catcatggaa tggttcagcg gcaagcccat
     3061 gccctggctg tcagcgcttg gcatgctggg tggcggcttc ggcatggcca tggtggtggc
     3121 cttcctgctg gctccacaac tcggtattct gggcaactgg gacttctcca acttccccgg
     3181 tggcctctgg tcgcagatta tcgcctttgg gattatcgtt gtctcagcag tgtggtacct
     3241 gctggtgaaa cgagcgcaga gcgcccgcgg catcaatgtc gaatatgcgt tcaaggagat
     3301 cccgcccgaa taaattaaac tttgtctcgt aactgcttag accccatttc tccgaccccc
     3361 gcgtgcccgt ggaggagcga agatagccga aaaaggggtg cacccccctt tttcggcctt
     3421 ttccccttgc ctgggggagt gggataggag ggatgaggaa tcaataaagt gcaccttcga
     3481 ggagatgcaa aaccatgaaa aatcaagcca gagttgtcgt cattggcggg ggcatctacg
     3541 gcgtcaacgt tgcctaccat ctggcaaaac tgggctggac tgacctggtc ctggttgaaa
     3601 aaaacgagat tgccagcgga gaatctgcac aagccgctgg tctggtaacc cagtttgcga
     3661 cttcccaaac catgatgcag ttccgcaagt acagcatcga actttacagc gagctgggtc
     3721 tgttcgacca tgtcggcagc ctgcgccttg cctccagcaa agaacagttg cacgagcttc
     3781 agcgcagtgt cagccgcgcc aagggcatcg gcatggatgt ggagatcatt tcgcccgccg
     3841 aagccctgaa aatcatgccc caattgagcc ccaaagatct ctacggggcg atttacctgc
     3901 cacgcgatgg tcatctcgac ccctacacca ccactacgaa tatggctcgc ctggtgcgcg
     3961 aaatgggttg cgaggtagtc accaacacgc gcgtcaccgg tattaagctt acccccaagg
     4021 gggaagtgag cgaggtggtt accacacgcg gctcgataaa gtgcgagcac gtgattaacg
     4081 ccaccggtct ttgggggccg cgcctggctg ctatggctgg gctgcatctg cccaccaccc
     4141 ccgtcgatca ccagcatatc gcccttaaag cggtgccggg gaacgagttc ccgcacagca
     4201 ccccctgcct gcgcgaccca gataacctgg tgtacatgcg cgaggagcag ggaggcctgg
     4261 tgatcggcgg ctacgaaccc aatccaaaag aacgctgggt gga
//
LOCUS       DBNHOH010000371         4392 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_277645, whole genome shotgun sequence.
ACCESSION   DBNHOH010000371 DBNHOH010000000
VERSION     DBNHOH010000371.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4392)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_277645"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1567
                     /locus_tag="AC16V7_06630"
     CDS             <1..1567
                     /locus_tag="AC16V7_06630"
                     /inference="COORDINATES: protein motif:HMM:NF019341.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6256831.1"
                     /translation="HRLVRAQSIQRVDGQTLSCYRFRHILFQKYLYGSLDEVERVHLH
                     EQVGTSLEGLYVAREEIVDIAVQLAMHYQKARIMERAIYYLHLAGDKAVQLSAYQEGK
                     AHLSRGLELLMSLPDSSQRDQQELALLISLGIAWKSDIPDPEGEKALIRARELCQRMG
                     KTTQLCRVLGELSVIPYVRAEYQKARELGEEALSLAEQAEDPLLVALGHWHLGYVLFG
                     LGEFTTSHAHLLQVISTYEPQQHHQPFVSLRGSDAGVSALAYDACCLWCLGYPDQALS
                     RSQEVLDLARDLGHPFSLADVLCFAGCLFNELRRDARALEQHAEELERLSRKMSFLSF
                     GETGTCYRGVALAMLGEIQEGIVLIRQGLAGRQSMEALCDFSGIMSALAAAHAKGGQP
                     EEGLATLDEALSLIEVTGERFLEAEHHRLRAELLLMQGDEEGAEASLESAIEVARRQS
                     AKSLELRATIDLARLWRERGRTDDARQVLGQIYNWFTEGFETIDLIEAKALLEEISHE
                     TIQDMDDLQSMSG"
     gene            1765..>4392
                     /locus_tag="AC16V7_06635"
     CDS             1765..>4392
                     /locus_tag="AC16V7_06635"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6256832.1"
                     /translation="MRLKIYLLGQFRIEVDERAIELSSRPAQSLLSYLALNVGVTQRR
                     EKLAGLLWPEASESNARSYLRQSLWRIHKSLERGGVKWEDYLEISELSVTFDKESAYW
                     LDAEQVLDVAEGRGVEVLKEAVRQYRGELLPGFYDEWVVVERERLQAAYHQRMSELLE
                     GLIEAREWDEVLKWGEEWIRYGYGPEAAYRGLMMAYAGMGDHGKVRASDQRCEEAMER
                     ELGMEPSPETEQLYEQLRRREVEGVGPGRVERGERGPAFLEEWEGVEEEKAVFVARER
                     ELAELDRILGQALGGEGRMIFITGEAGSGKTALVQEFSRRAQEAHAGLIVASGNCNAY
                     TGIGDPYLPFREILELLTGDVESRWVAGAISREHALRLWEVLPETGQALVEVGTDLID
                     TFVSGTSLIERVGGSMDGAGEWLVRLKEIVRGRPSGLGMPSLQQSNLFEQYARVLRAL
                     AQGKPMLIVLDDLQWADLGSISLLFHLGRRVGGSRILIVGAYRPEEVGLGRDGERHPL
                     EAVVHELQRAYGEIVVDVDQAERRVFVEAVLDSEANRLGVLFRDMLYRQTRGHPLFTI
                     ELLRGLQERGDLVQDQEGYWVEGSALDWERLPARVEAVIAERIGRLAEPLRAVLRVAS
                     VEGEQFTAEVVARVLGTGERQMVGQLSSELDRKHRLVRAQAIERLESRRVSRYRFRHS
                     VFQKYLYDNMDKAERAYLHEDLGNVLEEMYGDHSQEIAAIAPQLARHFLEAEMAKKAI
                     HYLHQASQRAVQLSAYQEALPHLIKGLELLMSLPNSPQRDQQELALQLGLGMAWIGVR
                     GFASQELKEAYLRARQLCQQMGDTRQLSRVLGELSLYYYVRAEHQNARELGEEALSLA
                     EQIEDPLLVALGHWYTGVSL"
BASE COUNT          938 a          929 c         1616 g          909 t
ORIGIN      
        1 gcatcgcctg gttcgcgcgc agagtatcca acgcgtggat gggcagaccc tgtcatgtta
       61 tcgcttccgg cacatcttgt ttcagaaata cctgtacggc agtctcgatg aggtggaacg
      121 ggttcacctt cacgagcaag taggcacttc cctggagggg ctgtatgtag ctcgggagga
      181 gattgtggat atcgccgtgc agttggcaat gcattatcag aaagcaagga taatggagag
      241 ggcgatctac tatctgcact tggccgggga taaggctgtg caattgtctg cctatcagga
      301 ggggaaagct cacctgagca ggggcttgga gttgcttatg agcttgcctg actcttcaca
      361 gcgagatcaa caagaactgg ctttgttaat ttcattggga atagcctgga agtcagatat
      421 tccagatccc gagggggaaa aagctttgat cagggcgcgt gagctatgcc agcggatggg
      481 taaaacaact caattatgcc gggtattggg tgaactgtcg gtaataccct atgtgcgagc
      541 cgagtatcaa aaggcacgag agcttggaga agaggcgctc agtcttgccg agcaggctga
      601 agacccgttg cttgtggcac tgggtcattg gcatctggga tatgtcttat tcggtctcgg
      661 agaattcaca acatctcacg cccacctttt gcaagtgatc tctacctatg agcctcaaca
      721 gcatcaccag ccattcgtgt ctctgcgcgg atctgacgcg ggtgtgagcg cgctagcgta
      781 tgatgcctgc tgcctgtggt gtcttggata tcccgatcaa gccctgagcc gcagccaaga
      841 ggtgctggac ttggcgcgag acttagggca tcccttctcg ctggccgatg tcctctgctt
      901 tgcgggttgc ctgttcaatg aattgcgtcg cgatgcgcgg gcgctcgagc agcatgctga
      961 ggagcttgag cgcttatcca ggaagatgtc cttcttgtcg tttggggaga cgggaacctg
     1021 ctaccgaggg gtagcgcttg ccatgctggg tgagatccaa gaagggatcg tactaatacg
     1081 ccagggcttg gcaggtaggc agtctatgga ggcactgtgt gacttttcag gaataatgag
     1141 tgctctggcc gctgcgcacg ccaagggggg gcaaccagag gaagggctgg caaccttgga
     1201 cgaggcatta tctctgattg aagtgacggg cgagcgtttt ttagaggctg agcatcaccg
     1261 cttgcgggca gagctgctgc tcatgcaggg cgatgaggag ggagccgaag ccagcctgga
     1321 aagtgcgatc gaggtcgccc gccggcagag cgctaagtcg ctggagctgc gggctacgat
     1381 tgacctggct cgcctgtggc gagaacgggg caggacggat gatgcgcgac aggtgttggg
     1441 gcagatctat aactggttca cggaaggctt cgagacgata gacctgatag aggctaaggc
     1501 tttgcttgag gagataagcc atgagaccat tcaagacatg gatgatctcc aatcgatgag
     1561 cggttagaaa gccggagccc atcgcaatca gaccgtcatt catgactatt agttagctgc
     1621 tttaaggtag accaacttgt atagctgggc taaaattatt acagttatgt gcccgccgaa
     1681 gtgtgtcgcg aaccaggaat ttatctcata ggctacgaat gtagggcgct atagggtagt
     1741 ttgtctgcgg attggaagta gggcatgagg ttgaaaatat acctgcttgg gcagttcaga
     1801 atagaggtcg acgagcgggc gattgagttg tcatcccggc cggcgcagtc gttgctgtcg
     1861 tatctggcgt tgaatgtggg ggtgacgcag cggcgggaga agctggctgg cctgctgtgg
     1921 ccggaggcga gcgagagcaa tgcgcgcagc tacctgaggc agtcgctgtg gcgcatccac
     1981 aaatcgctgg aaagaggggg agtgaaatgg gaagactact tggagatcag cgaactcagt
     2041 gtgacatttg acaaggaatc ggcgtactgg ctggatgcgg agcaggtgct ggatgtggca
     2101 gaggggcgtg gggtggaggt attgaaggaa gcagtccgtc agtatcgtgg ggagctgttg
     2161 cctggatttt atgatgagtg ggtcgtcgtg gagcgggaac gactgcaagc agcataccac
     2221 cagaggatga gtgagttact ggaaggattg atcgaggcga gggagtggga tgaggtattg
     2281 aagtggggag aggagtggat acggtatggg tatgggccgg aggcagccta ccggggattg
     2341 atgatggcct atgcggggat gggggatcat gggaaggtga gggcgagcga ccagcggtgt
     2401 gaggaagcca tggagcgcga acttgggatg gagccatcgc ctgagacgga gcagttgtat
     2461 gagcagctgc gccggaggga ggtggagggt gtgggtccag ggcgggtgga gcgaggtgag
     2521 cgagggccgg cgtttcttga agagtgggag ggggtagagg aagagaaggc ggtgtttgtg
     2581 gcgcgggaga gggagctggc ggagttggac aggatacttg ggcaagcgct gggcggagag
     2641 gggcggatga tattcatcac tggggaggca gggagcggga agacagcgct ggtgcaggag
     2701 ttcagccggc gggcacagga ggcgcacgcc ggactgatcg tggcgagcgg gaactgtaac
     2761 gcctacaccg gcatcggcga tccctacttg ccgtttcggg agatactgga gctactgacg
     2821 ggggatgtag aatcccgctg ggtggcaggg gcgataagca gggagcacgc cctgcggtta
     2881 tgggaggtgc tgccggagac ggggcaggcg ctggtggagg tggggacgga tttgatcgac
     2941 acatttgtgt ccgggacaag cctgattgag cgggtcgggg gtagtatgga cggtgcgggg
     3001 gagtggctgg ttcggttgaa agagattgtg cggggtaggc cgagtgggct ggggatgccg
     3061 agtctgcagc agagcaacct attcgagcag tatgccagag tgctgcgagc gctggcacaa
     3121 gggaagccaa tgctgatagt gttagacgac ttgcagtggg cggacctggg ttcgatcagc
     3181 ttgttgttcc accttgggcg gcgggtgggg gggagccgca ttttgattgt gggggcatac
     3241 cgtccggagg aggttgggtt ggggagggat ggggagcgtc atccgctgga ggcggtggtc
     3301 catgagctgc agcgagcgta tggggagatc gtggtggacg tggatcaggc agagaggcgt
     3361 gtgtttgtgg aggcggtgct ggacagcgag gcaaaccggt taggggtgct gtttcgggac
     3421 atgctgtacc ggcagacccg ggggcatcca ttgttcacga ttgagttgct gcgggggctg
     3481 caggagcgag gggatttggt gcaggatcag gaggggtact gggtagaggg atctgcgttg
     3541 gactgggaga ggctgccggc gcgggtggag gcggtgattg cggagcgtat tggacgattg
     3601 gctgaaccgc tgcgggctgt gttgcgggtg gcgagtgtgg agggagaaca attcactgct
     3661 gaggtggtag ctcgagtcct aggaacgggt gagagacaga tggtagggca gttgagcagc
     3721 gagctggacc gcaagcatcg cctggttcgc gctcaggcga tcgagcgttt ggagtcgcgg
     3781 cgtgtatccc gttaccgatt caggcattct gtgttccaaa aatatctgta cgataacatg
     3841 gacaaagcgg aacgggcata tctccacgag gacttgggaa atgtgctcga ggagatgtat
     3901 ggagaccact cgcaagagat cgcggcgatc gctccgcaat tagcgcggca cttcctggaa
     3961 gcagaaatgg caaagaaggc gatccactat ctgcaccagg caagtcaaag ggctgtgcaa
     4021 ctgtccgcct atcaggaggc gctgcctcac ttgatcaagg gcttggagtt acttatgagc
     4081 ctccccaatt ccccgcaacg cgatcagcaa gagctggcgc tgcaactggg attgggcatg
     4141 gcgtggatag gcgtccgggg gtttgcatcg caggaattga aagaagctta ccttagagcc
     4201 cgccagctgt gccagcagat gggtgatacg agacaactct cgcgggtgct gggggagctg
     4261 tcgctgtatt actatgtacg ggcagagcat cagaatgcgc gagagctcgg agaagaggcg
     4321 ctcagcctgg cagagcagat cgaagaccca ctgctcgtag cattgggaca ctggtacaca
     4381 ggcgtttcct tg
//
LOCUS       DBNHOH010000372         1832 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1367966, whole genome shotgun sequence.
ACCESSION   DBNHOH010000372 DBNHOH010000000
VERSION     DBNHOH010000372.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1367966"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /gene="glnS"
                     /locus_tag="AC16V7_06640"
     CDS             complement(<1..931)
                     /gene="glnS"
                     /locus_tag="AC16V7_06640"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HIR6256833.1"
                     /translation="MAPEETTPPSDFIREIVREDNRTGKFGGRVHTRFPPEPNGYLHI
                     GHAKSIVLNFGIAEEFGGKCNLRFDDTNPTKEEVEYVESIIQDVRWLGYDWEDRLFYA
                     SDYFAQLYEWAVKLIKKGEAYVCDLTADEVREYRGTLTQPGRESPYRDRSVEENLDLF
                     ARMRAGEFPDGARTLRAKIDMSSGNLNMRDPVLYRILHESHHRTGDVWCIYPMYDFAH
                     GQCDSIEDITHSICTLEFQDHRPLYDWFLDELEVYHPQQIEFARLNLSYTLLSKRKLL
                     RLVKDGHVNGWDDPRMPTLSGLRRRGYTPQAIRD"
     gene            1296..1366
                     /locus_tag="AC16V7_06645"
     tRNA            1296..1366
                     /locus_tag="AC16V7_06645"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1328..1330,aa:Gln,seq:ctg)
     gene            1463..>1832
                     /locus_tag="AC16V7_06650"
     CDS             1463..>1832
                     /locus_tag="AC16V7_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019119467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR6256834.1"
                     /translation="MTTQFEVHDPAILSQARGVLDACKTSQEMYQAVLDAVKRNEQWR
                     GQHCINLLAPEAPTSPTVRALLSSEVGIRAAEGHIGALNRWFVGTHYIDEIEALCIEL
                     LKQVFKVRYADHRLMGSMLGN"
BASE COUNT          424 a          506 c          469 g          433 t
ORIGIN      
        1 aatcgcggat cgcctgcggc gtatagccgc ggcggcgcag acccgagagg gtcggcatgc
       61 gcgggtcatc ccagccgttg acgtgcccat ccttaaccaa acgcagcagc ttacgcttgg
      121 agagcaatgt atagctcagg ttaagccggg caaactcgat ctgctgtgga tggtaaactt
      181 ccagctcatc caggaaccag tcgtacagag ggcgatggtc ctgaaattct aacgtgcaga
      241 tggaatgggt gatatcctcg atggagtcgc actgaccgtg ggcaaagtcg tacatcgggt
      301 aaatgcacca tacatcgcca gtacggtggt gagattcgtg caagatgcgg tacagcactg
      361 ggtcgcgcat attcagattg ccggaagaca tgtcgatctt ggctcgcaag gtgcgcgccc
      421 catcagggaa ttcaccggcg cgcatgcggg cgaacaagtc caggttttcc tcaaccgagc
      481 ggtctcggta agggctctct ctgcccggct gggttaacgt accgcgatac tcgcgcacct
      541 cgtccgcagt gagatcgcac acataagctt cacccttctt aatcagcttg accgcccatt
      601 catagagctg tgcaaagtaa tctgaggcat agaagagtcg atcttcccaa tcgtatccca
      661 gccagcgcac gtcctgaatg atcgactcga catattcgac ctcttccttg gtagggtttg
      721 tatcgtcaaa gcgcaggttg catttgccgc caaattcctc agctattcca aaattaagca
      781 cgatcgattt ggcatgacca atgtgcaggt agccgtttgg ctcaggtgga aaacgagtgt
      841 gcacgcgacc accaaacttg cctgtgcggt tatcttcccg gacaatttca cgaataaagt
      901 cgctgggagg ggttgtctct tcgggtgcca tagcagcgcc tctcttatga cctatttttg
      961 gatggtaact taatgcacaa attataacaa acctatggcg ttaatacccc tctcattgat
     1021 ctcctcctgc cctaatcccg ctgctttgcc cttgaacagc ctaacacaaa tttgttaagc
     1081 ttatgaaagg cttcccctca gaagtgcaag ggtgtaactg agaatagcct gctatacttg
     1141 ccaagccaac atctttctac tgtcgagaaa gggttggatt tgggagccgg gcgcaggcct
     1201 ggctccctta tttattacac ccattctaac gatctcggtt cgccccacct ttgacgaggc
     1261 cccccttaca tggtacaatc tccatcgtcg aacgctgggg gttcgtctag tggtaggaca
     1321 gcagactctg aatctgtatg ccgaggttcg aatcctcgac ccccagcctg acaccttttt
     1381 atcgctccag cactaaagca ccccatggga tatccttggg gtgttatcta tcatccaatg
     1441 agctatcgaa aggaatacct cgatgaccac acaatttgag gtgcatgatc ccgccattct
     1501 atcccaggcg cgaggcgttt tagatgcctg caaaacatcg caggagatgt accaggcggt
     1561 tctggatgct gtcaagcgca acgagcaatg gcgcggtcag cattgcatca acctgctggc
     1621 ccctgaagcc cccactagcc ctaccgtacg cgcgctgctt tccagtgaag tgggcatccg
     1681 cgcggccgag gggcacatcg gcgccctaaa tcgctggttc gtaggcacgc attatattga
     1741 tgagatcgaa gccctgtgca ttgagctttt aaaacaagta ttcaaggttc gctacgcaga
     1801 tcatcgcttg atgggcagta tgctgggtaa cc
//
LOCUS       DBNHOH010000373         2244 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1623897, whole genome shotgun sequence.
ACCESSION   DBNHOH010000373 DBNHOH010000000
VERSION     DBNHOH010000373.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1623897"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="AC16V7_06655"
     CDS             <1..329
                     /locus_tag="AC16V7_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256835.1"
                     /translation="KSAGTGYQANLRYGSQQSITRDWSMPMHQTDTLFHKGKVAMQFI
                     FGGEADNHALNTVPKETLVKITKAEPGGLDGKGPWEPTKTGFTPGHEGDFMQKYLAGG
                     AVEIKK"
     gene            354..617
                     /locus_tag="AC16V7_06660"
     CDS             354..617
                     /locus_tag="AC16V7_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256836.1"
                     /translation="MPYEEFDVEDPMDLVGMVLPGEPGQLEAMAECFVEEYVRMGWNE
                     TRLMTLFVNPMFMATHRIYRQKGEKYVKELIQRVGSKYQLKTG"
     gene            658..1917
                     /locus_tag="AC16V7_06665"
     CDS             658..1917
                     /locus_tag="AC16V7_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018047899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR6256837.1"
                     /translation="MAEVYNWQLGRNMSYVFDEAHPQKQFVAIFNINRCIGCQTCTGA
                     CKATWTFSKGQEYMWWNNVETKPYGGYPQHWDIKILQILDEAHKTAGVKAEWDTSQTS
                     ENAPYGVYNGMTITEAGGDNQQALGYIPPDTEWRAPNFYEDTSTAYKGDALGISKEGV
                     SLPEHQTWFFYLQRICNHCTYPACAAACPRKAVYKRPEDGIVLIDQARCRGYRKCVEQ
                     CPYKKSMYRGTTRTSEKCVACYPRVEGTDPLTDGVPMETRCMAVCPGKIRLNGLVDIG
                     PDGSWAEDPQNPLYYMIQVEKVALPLYPQFGTEPNIYYIPPRWAPRSYLRQMFGPGVD
                     EAIARYAAPSRELLAVMQLFRTTQKMIFRYEIEEGPLVLETEVNGKPWQMYNDTVIGF
                     DAQGREATRLSVVEPIYERPADRMNSI"
     gene            1914..>2244
                     /locus_tag="AC16V7_06670"
     CDS             1914..>2244
                     /locus_tag="AC16V7_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256838.1"
                     /translation="MITDNAIRRAQLYCFLADAFLYPAENWLEDLPALTPILRDLELA
                     ELSPPTFDLQLSELQSEHRRSFGLTGSLCYETEYGLPNEYRQSQELADIAGFYQAFGF
                     KVGGLRRE"
BASE COUNT          540 a          601 c          634 g          469 t
ORIGIN      
        1 caaagtcggc ggggacggga tatcaagcca acctgcgtta tggctcgcag cagtcgatca
       61 cgcgtgactg gtctatgccc atgcaccaga ccgatacgct cttccacaag gggaaggtag
      121 ctatgcagtt cattttcggt ggtgaagccg acaatcacgc cttgaacacc gtccccaaag
      181 agaccctggt aaagatcacc aaagccgagc ccggaggttt ggacggcaaa ggcccctggg
      241 agccgaccaa gaccggcttt acccctgggc atgaaggcga cttcatgcag aaatatctgg
      301 ctggtggggc ggtagaaata aagaaatgat tggtgtgcac actggaggaa agaatgccat
      361 acgaagaatt tgatgtagaa gatccaatgg acctggtcgg gatggtgctg cccggcgagc
      421 caggccagct tgaagcgatg gcggaatgct tcgtggaaga gtatgtgcgc atgggttgga
      481 atgaaacacg cctgatgacc ctctttgtca acccgatgtt catggccacg caccgcattt
      541 atcgccaaaa gggtgagaag tacgtgaaag agctgatcca gcgagtgggc tcaaaatatc
      601 agctcaagac aggctagtca tgctaggaaa attgctcgca ccaaggagtt aatcgatatg
      661 gctgaagtct acaactggca actaggccga aatatgagtt acgttttcga cgaggcgcac
      721 ccccagaaac agtttgtcgc catctttaac attaaccgct gtattggctg ccagacctgt
      781 accggggctt gcaaagctac ctggaccttt tcgaaaggtc aggaatacat gtggtggaac
      841 aatgtggaga ccaaacccta tggtggctac ccccagcact gggatatcaa gatattgcag
      901 atactggacg aggctcacaa gaccgctggc gtaaaggctg agtgggatac atcccagacc
      961 agcgagaacg ccccttacgg tgtctacaat ggaatgacca ttaccgaagc gggtggcgat
     1021 aaccagcaag cgctggggta tatccctcca gacactgagt ggcgagcccc aaatttctac
     1081 gaggacacgt ctactgcata taagggcgac gcgctgggta tcagcaagga gggcgtatcg
     1141 ctgcccgaac accagacctg gttcttctac ttgcagcgca tctgcaacca ttgtacctat
     1201 ccagcctgtg cagcggcttg cccgcgcaag gcggtttaca agcgcccaga ggatgggatc
     1261 gtgctgatcg accaggcgcg ctgccgcggt tatcgcaagt gcgttgaaca atgcccctat
     1321 aaaaaatcga tgtatcgcgg cactacccgc accagtgaga agtgcgtggc ctgttacccg
     1381 cgtgtggagg gcactgaccc gcttacggat ggcgtgccta tggagacgcg atgcatggca
     1441 gtctgccccg gcaagattcg cttgaatggt ctggtagata tcggcccgga tggctcgtgg
     1501 gcagaagacc cacagaaccc actgtactac atgatccagg ttgaaaaagt ggcgctgcca
     1561 ctctacccgc aatttggcac cgagcctaat atttattaca tcccgccgcg ctgggcgccg
     1621 cgctcctacc tgcgccagat gtttggtcca ggggtggatg aggcgatcgc tcgctatgcg
     1681 gcgccatcgc gtgagctact ggctgtcatg cagttattcc gcaccaccca gaagatgatc
     1741 ttccgctatg agatagagga aggcccactg gtgctggaaa ccgaggtcaa cggcaagcct
     1801 tggcagatgt ataacgatac cgtgatcggt tttgatgctc agggccggga agccacgcgc
     1861 ttgagcgtgg ttgagccaat ctacgaacgc ccggcagata ggatgaattc gatatgatca
     1921 cagataatgc cattcgccgt gctcaattgt attgcttcct ggctgatgct tttctctatc
     1981 ctgcggagaa ctggttggaa gacctgcctg cgctgacccc gatcctccgc gatctggagc
     2041 tggctgaact ctccccccca accttcgatc ttcagctatc tgagcttcaa tctgagcacc
     2101 gccgctcatt tggcctgacg ggttctctat gctacgaaac agaatatggt ctgccaaacg
     2161 aatatcgcca gtcccaagaa ctggccgata ttgccgggtt ctaccaggcc ttcggtttca
     2221 aggtgggcgg gcttcgacgc gaac
//
LOCUS       DBNHOH010000374         3183 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1752490, whole genome shotgun sequence.
ACCESSION   DBNHOH010000374 DBNHOH010000000
VERSION     DBNHOH010000374.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1752490"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="AC16V7_06675"
     CDS             complement(<1..512)
                     /locus_tag="AC16V7_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/alpha barrel domain-containing protein"
                     /protein_id="HIR6256839.1"
                     /translation="MIDLTTSYLGLSLKNPLVASASPLSKQLDSARQLEDSGVSAIVM
                     YSLFEEQIIQDSFKLDRDLDRGPYSSAEAFRYFPDYGMYSIGPESYLQHLRRVKEAVS
                     IPVIGSLNGVSSGGWVEYAQKIEQAGADALELNLYDLATDVSLTSAELEEQHIRLVED
                     VSKEIKIPLAV"
     gene            complement(582..>3183)
                     /gene="nifJ"
                     /locus_tag="AC16V7_06680"
     CDS             complement(582..>3183)
                     /gene="nifJ"
                     /locus_tag="AC16V7_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HIR6256840.1"
                     /translation="EPLYQDVVTAINEGLAEGSVPYTQFAHRPYPRVIGGRYGLSSKE
                     FTPAIVKGIFDELRKAEPKNHFTVGINDDVTFSSIEYDPSFTIDMPDTVRCVFWGLGA
                     DGTVGANHNSIKIIGEETDNYAQGFFVYDSKKSGTVTVSHLRFGPRPIRAPYQISKAN
                     FVACHQFSFLERLDVTKYAAPGAVFLLNSIYGPDEVWDKLPVETQIDIIEQKLHFYVI
                     NAYDVARETGMGGRINTIMQTCFFAISGILPRDQAIAEIKHAIERTYGKRGKAVVQAN
                     FAAVDKTLENLFEVKVPEKVSSQLTRRPAVAAQAPEFVRNVVGKMIAGEGNSLPVSAL
                     PIDGTFPSATTQWEKRNIALEIPVWEPDICIQCGKCTFVCPHAVIRAKVYEPSHLANA
                     PEGWQADEARFKEFPDKMYTLAVAPEDCTGCGLCIEICPVKDKTQVGRKAINLAPQPP
                     IRDAECVKWDYFLTIPEVDRTQMNLKVVKNSQLLQPLFEFSGACTGCGETPYVKLLSQ
                     LFGDHIVVANATGCSSIYGGNLPTTPWAINKEGRGPAWSNSLFEDNAEFGLGMRLTLD
                     KHNEYARELLVRLADVVGETLVDELLKADQTSESGIQAQRERVKLLKEKLNGVDDPRA
                     HDLLSLCDVLVRKSVWIVGGDGWAYDIGYGGLDHVLASGRNVNVLVLDTEVYSNTGGQ
                     ASKATPRAAVARFAAQGKALPKKDLGMIAMAYGYIYVARVAMGASDQQTLNAFMEADA
                     YDGPSLIIAFSHCIEHGYDLRKGLDQQKLAVLAGHWPLYRFNPSLIAEGKSPLSIDSK
                     GPSIPYEQYAYNETRFKVLLHSNEQRAESLMKLAQKDARTRWELYQQMAQMHYDGSAE
                     KNGKNGDNDS"
BASE COUNT          744 a          885 c          777 g          777 t
ORIGIN      
        1 accgccaggg ggatttttat ttccttgctc acatcctcta ctaaacggat gtgttgctct
       61 tccagctcag cgctggttaa ggaaacgtcg gttgctaaat cgtacaggtt caactccaga
      121 gcatcagctc cggcttgctc gattttttga gcgtattcca cccaaccgcc agaagaaacc
      181 ccattcaggc ttccgatgac agggatgctc acggcttcct taacccggcg caaatgctgc
      241 agataactct caggaccgat actgtacatg ccgtaatctg gaaaatagcg aaacgcctcc
      301 gcagatgaat atggcccccg atccaggtca cgatccaact taaagctatc ctggatgatt
      361 tgctcctcga acaatgaata catcacgatc gcagatacac cagaatcttc cagctggcgg
      421 gctgaatcca gctgcttcga aagaggggag gcagaagcca ccaagggatt ttttagggat
      481 aaacccaggt aagacgtggt cagatctatc atatataact cctcattata aaggggaatt
      541 agggatagga tgtagctcct attctaatcc cacctcccta cttaggaatc gttatctccg
      601 ttctttccat tcttctctgc agagccatcg taatgcattt gggccatctg ctggtacagc
      661 tcccagcgcg tcctagcatc tttttgagcc agcttcatca aggattcagc cctctgctcg
      721 ttgctgtgca acagcacctt gaagcgtgtc tcgttatacg cgtactgctc gtatgggata
      781 ctgggacctt tcgagtcaat actgagcggg cttttccctt ctgcaattaa ggaggggttg
      841 aaacggtaca gtggccagtg accagccaga acggcaagtt tttgttgatc cagtcctttg
      901 cgcaagtcat aaccatgctc aatgcaatgg ctaaaggcga taatcaagga tggcccatcg
      961 taggcatctg cctccatgaa agcgttgagc gtttgctggt cgctggctcc cattgcgacg
     1021 cgagccacat agatatatcc atacgccata gcaatcatgc ccaggtcctt ctttggaagg
     1081 gctttgcctt gagcggcgaa gcgggcaact gcagcgcgtg gggtagcttt agatgcctga
     1141 ccacctgtgt tcgagtacac ctcagtatcc agtaccaaca cattcacatt tcgcccggag
     1201 gcgagcacgt gatccaaacc gccatatcca atgtcatacg cccagccatc acctccgacg
     1261 atccatacac tcttccgcac caggacatcg cataggctga gcagatcatg cgcgcgtggg
     1321 tcgtccacac cgttaagttt ttccttgagc agtttcacac gctctctttg tgcttggata
     1381 ccgctctcac tagtctggtc cgctttgagc agctcgtcaa ccagtgtctc gcccaccacg
     1441 tctgccagcc gaaccagcag ctcgcgagca tactcgttgt gtttatccag cgtcaggcgc
     1501 atacccaaac cgaactcagc gttgtcctcg aaaagagagt tcgaccaggc tggaccgcgg
     1561 ccttccttgt ttatagccca gggtgtggtg ggcaaatttc cgccgtagat cgaagagcaa
     1621 cccgttgcgt tcgctaccac gatatggtct ccaaagagct gggagagtag ctttacatag
     1681 ggtgtctcac cacatccagt acaggcaccg ctaaactcaa acaaaggttg cagtaactgt
     1741 gaatttttca ctaccttgag attcatttgg gtgcggtcta cttcaggtat ggtcaggaaa
     1801 taatcccact tgacacactc agcatcgcgt atcggcggct gcggcgccaa attaatcgcc
     1861 ttcctcccca cctgggtctt atctttcacc gggcaaattt caatacacaa accacatccc
     1921 gtgcaatcct cgggcgccac cgctaaggta tacatcttat ccgggaattc tttgaaacgt
     1981 gcttcgtctg cttgccatcc ctccggcgca ttcgccaagt gagaaggctc atatactttg
     2041 gcgcggataa cagcatgcgg gcagacaaag gtgcacttgc cgcactgaat gcatatatcc
     2101 ggttcccaca ccgggatctc cagggcgata tttctcttct cccactgcgt ggtagccgac
     2161 gggaatgtgc catcaattgg cagagcgctt acaggcaggc tattaccctc gccagcaatc
     2221 atctttccga ctacattgcg cacgaactct ggtgcctggg cagccaccgc tggacgacga
     2281 gtgagttggc tagacacttt ctcagggact ttcacttcga acaggttctc caaggttttg
     2341 tcgacagctg cgaagttggc ttgtaccacg gctttaccgc gcttgccata ggtccgctca
     2401 atagcgtgct tgatctcggc gatcgcctga tcgcgtggca atattccgct gatggcgaag
     2461 aagcaggttt gcataatggt attgatgcga ccacccatgc ccgtctcgcg ggcgacatcg
     2521 taagcgttaa tcacataaaa gtgcagcttc tgttcaatga tgtctatttg cgtttctacg
     2581 ggcaatttat cccaaacttc atcgggacca tagatgctgt tgaggagaaa gactgcccct
     2641 ggagcggcgt acttggttac atctaaacgc tccaagaagg aaaactggtg acatgcgacg
     2701 aagttcgcct tgctaatctg gtacggagca cggatcgggc gcgggccaaa acgcaaatgc
     2761 gagacggtca ccgtgccaga tttctttgag tcgtacacaa agaagccctg ggcatagttg
     2821 tcggtctcct cgccaatgat tttgatcgag ttatggttgg cgcccaccgt cccatccgcg
     2881 ccaagccccc agaacacaca gcgcacagta tcgggcatat caatggtaaa tgaagggtcg
     2941 tattcaatac tgctgaaagt cacatcgtca ttaatgccca cggtgaagtg attctttggt
     3001 tctgctttgc ggagctcatc aaatatgccc ttgacaatgg caggggtaaa ctctttcgat
     3061 gaaagaccat aacgaccgcc gatcacacgc gggtacggtc gatgcgcaaa ctgggtatac
     3121 ggcacgctgc cttctgccaa gccttcgttg atggcggtga ccacgtcttg gtagagcggc
     3181 tct
//
LOCUS       DBNHOH010000375         1336 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_727477, whole genome shotgun sequence.
ACCESSION   DBNHOH010000375 DBNHOH010000000
VERSION     DBNHOH010000375.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_727477"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..1115
                     /locus_tag="AC16V7_06685"
     CDS             174..1115
                     /locus_tag="AC16V7_06685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6256841.1"
                     /translation="MNDFPDIHPSRPELNLLTPSGWVDYALLDSGNGLKLERYGPYTF
                     VRPEPQAIWQPAASQESWQAAHAIFQPTREESGGHWQFNKPVKESWVMQYKGLKFRVQ
                     TTAGRHLGVFPEQAPHWDWIGNVVQTAKRDVNVLNLFGYTGLASLEAARAGAHVTHID
                     ASKKSVAWARENQALSDLGNKPIRWIVDDALKFVQRDARRGVKYDGLILDPPKFGRGP
                     KGEVWEFYRLLPELLSACRSVLSQEPIFICLTAYAIRASALSLYYSLQEMVAGFAGQI
                     SVGELALEEHSAGRMLSMAIFARWVGETILGAQGKFI"
BASE COUNT          334 a          327 c          370 g          305 t
ORIGIN      
        1 gggggtcagc ctggctttga cgatctcgta caccttgggg tttgtggttg ccatcacgca
       61 gcaggcatcc atctggggga cactctatgg ttggatcact gcaggggtat tcctggtact
      121 gaccgccggt tacgcttata ttctggtaaa gaaaaattcc gagtaagcat ttgatgaacg
      181 actttccaga tatacacccg agcagacctg agctaaactt gcttaccccg tccggctggg
      241 tggattacgc cctgttagat agcgggaatg ggctaaaatt ggagcgctat ggcccataca
      301 ctttcgtgag acccgaaccg caggctatat ggcagccggc agcatcccag gaaagctggc
      361 aggcggctca tgcaatattc caacccaccc gcgaggagag cggcgggcac tggcagttca
      421 acaagccagt caaggaaagc tgggtgatgc aatataaagg gttgaagttt cgcgtccaga
      481 ccaccgctgg ccgccacctg ggcgtttttc ctgaacaagc gccgcattgg gattggatcg
      541 ggaatgtcgt gcagactgcc aagcgcgatg taaacgtgct caatttgttt ggctacactg
      601 gcctggccag cctggaggcg gccagagctg gagcgcacgt cacccatatc gatgcctcca
      661 aaaagtccgt cgcttgggca cgagagaacc aggccctctc ggatctagga aataagccta
      721 tacgctggat tgtggacgac gctctgaagt ttgtgcagcg cgatgccagg cgaggcgtca
      781 aatatgatgg tctgatactt gacccaccca aatttggacg cggaccgaaa ggcgaggtgt
      841 gggagtttta ccgattactc cccgaattat taagcgcctg ccgtagcgtc ctcagccaag
      901 agcctatctt tatatgcctg accgcctacg ccatccgcgc ctcagccttg agcctgtatt
      961 acagcttgca ggagatggtt gccgggttcg ctggacagat cagcgtcggt gagctcgccc
     1021 tcgaagagca cagtgcagga aggatgcttt cgatggcaat ctttgcccgc tgggtgggtg
     1081 agacgatcct gggtgcccag ggaaagttta tctaggacaa attcaagcca tgaagttttt
     1141 gagcatttaa aatttcttaa aaattaaatc acaacgtatt taccatatat aatgaaagta
     1201 ttatgcgccc catgaagtaa taagataaaa taaatttcac caaaaagtat tggaggtcaa
     1261 tcatggcaga cgtgatcgaa tataaaatct ttggcgatga cctgcaactg gtcgaggtag
     1321 agctggatcc tggaga
//
LOCUS       DBNHOH010000376         2373 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_406385, whole genome shotgun sequence.
ACCESSION   DBNHOH010000376 DBNHOH010000000
VERSION     DBNHOH010000376.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_406385"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="AC16V7_06690"
     CDS             <1..388
                     /locus_tag="AC16V7_06690"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF013575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0106029 - tRNA pseudouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HIR6256842.1"
                     /translation="RYAWRVWPALDLDLLQQATSLLVGRQDFAAFGSPPRPSGSTQRT
                     VFQAQWTRYNHELIFLITADAFLYRMVRRLVSFQVEIGQGRRELEALRQVLELPPDSA
                     VQGLAPAHGLTLLKVVYPSDEARILI"
     gene            494..922
                     /gene="rplM"
                     /locus_tag="AC16V7_06695"
     CDS             494..922
                     /gene="rplM"
                     /locus_tag="AC16V7_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIR6256843.1"
                     /translation="MEKTYYPKAGEISQDWILVDANDQNLGRLATHIATLLLGKHKPN
                     FTPGVEMGDYVVVINAERIRVTGKKLVEKKYYRHSNYPGGLKTISLQDQLAKHPDRVI
                     KSAVWGMLPHNKVGRRLLKNLKIYAGPEHPHAAQTPKSLS"
     gene            935..1330
                     /gene="rpsI"
                     /locus_tag="AC16V7_06700"
     CDS             935..1330
                     /gene="rpsI"
                     /locus_tag="AC16V7_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIR6256844.1"
                     /translation="MSTQFYEGIGRRKQATARVRIMSGSGNFTVNEKPAEAYFTRLGD
                     LEQIVAPLKAAGENPNQLDISVVVDGGGVTGQTDAVQLGVARAILLMNPDMRSTLRRG
                     GYLTRDARVKERKKPGLKRARKAPTYTKR"
     gene            1421..>2373
                     /locus_tag="AC16V7_06705"
     CDS             1421..>2373
                     /locus_tag="AC16V7_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR6256845.1"
                     /translation="MDNSSHGGIILLGLGPGDPALLTRQAWELLNNIQVIHLRTSQHP
                     VVAGFPPSLRVLSFDDLYERAENFEDVYTQIIARILELGKSPDGVIYAVPGHPFVAEA
                     TCPEIARRARLDGIPVRVLEGISFLEPCLTALELDPFPHLALIDALELASAHVPSFPP
                     SSPAIIAQIHSRAVASDVKLTLMSLYPDEHPVKLVHAAGTPDELVEAIPLFEIDRSEH
                     TGLLTALYVPPLGAATSFEAFQEVIAHLRAPEGCPWDKEQTHQTLRSHLIEESYEVLA
                     ALDAGDPQAMREEFGDLLLQIVLHAQIASEFGEFTMSDVLE"
BASE COUNT          549 a          626 c          656 g          542 t
ORIGIN      
        1 tcgatacgcc tggcgggtgt ggcctgccct ggatctcgac ttgctccaac aggctacgag
       61 tctcttggtc ggaaggcagg attttgccgc atttggttcc ccgcctcgcc cttcaggaag
      121 cacacagcgg acggtctttc aggcacaatg gacccggtat aaccacgagc tgattttttt
      181 gatcactgct gatgcatttc tttatcggat ggtgcggaga ttggtcagct tccaggtgga
      241 gatcgggcag ggaaggcgtg agcttgaagc gttaaggcaa gtgctggagt tacccccaga
      301 ctcagcggtt caaggcttgg cgccagccca tgggctaacc ctgctaaagg tggtctaccc
      361 atccgatgaa gcgaggatac tcatttagga aatatgcagt taagttatca atctaactgc
      421 atcacgcggt cattgatgaa atagatcaga aattgtcagg ctaaatgact ggaagatagc
      481 ggagaatttg aacgtggaaa aaacttatta tccaaaagct ggtgaaatca gccaagattg
      541 gatcctggtt gacgcaaatg atcaaaacct gggacgtctg gcgacacata ttgctactct
      601 tttgctgggt aagcataagc caaacttcac tccgggcgtg gagatgggag attacgtcgt
      661 cgttattaat gcggagcgca tccgcgtaac cggaaagaag ttggttgaga aaaaatatta
      721 tcgccattcc aattaccccg gtgggctgaa gaccatcagc ctgcaggatc agctcgctaa
      781 gcatccggat cgagtgatca aaagcgctgt atggggtatg ctgccgcaca ataaagtcgg
      841 caggcggttg ttgaaaaatt tgaaaattta tgctgggccc gaacatccac atgctgccca
      901 gacacccaag tcattgtctt aaggagcaag aagcatgtcg acgcaatttt atgaaggcat
      961 tggtcgccgc aagcaagcta ccgcgcgcgt gcgcatcatg tcaggatcgg gaaatttcac
     1021 ggttaatgag aagccagccg aggcttactt cacccgcttg ggtgatttgg aacaaattgt
     1081 cgctccccta aaagcggctg gagaaaaccc taaccagctg gatatatccg ttgtggttga
     1141 tggcgggggt gtcacagggc aaacagacgc tgtccaattg ggcgtggcgc gcgccattct
     1201 cttgatgaac cctgatatgc gctcgacgct ccgtcgaggc ggatacctga cccgtgacgc
     1261 gcgtgtcaag gagcgcaaga agcccggtct gaagcgggct cgcaaggctc ccacctacac
     1321 caagcggtaa ttcgtacaat ttcacggctt aagaatgata ttggggtaat gcgcaggcga
     1381 gatctgccca ttaccccaaa agtcttttaa aatagtggct atggacaact catcacatgg
     1441 tggaattatt cttcttgggc ttggcccagg cgatccggcc ttgctcaccc ggcaggcatg
     1501 ggagcttctc aataacatcc aggtaattca cctgcgcacc agccagcatc ccgtggtggc
     1561 aggtttccct cccagcctga gggtactatc attcgatgat ctgtatgagc gcgcagaaaa
     1621 tttcgaagat gtttacacgc agatcattgc tcgcatctta gagctgggga agtcgccaga
     1681 tggtgtgata tatgccgtcc cggggcatcc ttttgtggcc gaagccacct gtccggagat
     1741 tgcccgccgc gctcgcttgg atggcatccc ggtgcgcgta ttggagggga ttagctttct
     1801 ggagccatgc ctgacagctc ttgaattaga cccctttccc cacctggctc tcattgacgc
     1861 cctggagctg gcatctgccc atgttccatc cttcccgcca agctccccgg caatcatcgc
     1921 ccaaatacac tcgcgggcgg ttgcttcgga cgtcaagctc actttgatga gcctgtaccc
     1981 tgacgagcat ccggtcaagc tggtacatgc ggcaggcacg cccgatgaat tggtggaagc
     2041 aatcccgcta ttcgaaattg accgtagcga gcacactggg ctgctcactg ccttgtatgt
     2101 tcccccatta ggtgccgcga cctcctttga ggcttttcag gaggtgatcg cccacctgcg
     2161 cgcgccggaa ggctgccctt gggacaagga gcagacccac caaacgcttc gctcccacct
     2221 gattgaggaa agctacgagg tcctggctgc gctggatgca ggcgatccac aggctatgcg
     2281 cgaggagttc ggcgacctgc tgctacagat tgtgctgcat gcccagattg ctagcgaatt
     2341 cggcgagttc accatgtcgg acgtgctcga aag
//
LOCUS       DBNHOH010000377         2154 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_406392, whole genome shotgun sequence.
ACCESSION   DBNHOH010000377 DBNHOH010000000
VERSION     DBNHOH010000377.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_406392"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="AC16V7_06710"
     CDS             complement(<1..769)
                     /locus_tag="AC16V7_06710"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6256846.1"
                     /translation="MPAPRLHRLPTNVWVLSAASFLTDVSSEMLNNLLPLFLYNVLGV
                     GTAIIGLIDGIAETTASLVKIYSGVFSDRIGKRKPLTISGYLLSTLAKPFLYLATSWG
                     WVLGVRFVDRIGKGIRTAPRDALLAGSVSTEQRGFAFGLHRASDTAGAFTGLGIAAYL
                     IWINQAGKAQLTQATFQWVVLASILPAALAVLILVIGARDVEATKTANSIVPTFRFTW
                     NNLDRRIKAFILVIALFTLGNSSDAFIILRSQERGLSV"
     gene            complement(784..1464)
                     /locus_tag="AC16V7_06715"
     CDS             complement(784..1464)
                     /locus_tag="AC16V7_06715"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6256847.1"
                     /translation="MAIDKYPRPSLTVDIVIFTLRENRLQVLLIRRANQPFAGYWALP
                     GGFVQVDESLEDAAARELSEETGIEQAYLEQLYTYGDPERDPRGRVVSVAYFALIPAD
                     ASVRSEGGSDASQASWFPLDELPDLAFDHAEIISYAIRRVRYKLEYTAVGFELLPESF
                     TLSEIQKTYEIILGEPLDKRNFRRRMLESGIIEPTQFKRGKGGRPARLYRYREDAVAE
                     IKARRLFP"
     gene            complement(1477..>2154)
                     /locus_tag="AC16V7_06720"
     CDS             complement(1477..>2154)
                     /locus_tag="AC16V7_06720"
                     /inference="COORDINATES: protein motif:HMM:NF014476.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256848.1"
                     /translation="GLPYAVMDCTWITAYRTAAATALSARFLARPESSVAGILACGVQ
                     GRTNLEALKAIFPIQRAYAYDIVPEVQKRYVEEMSKKLDLEIIGVDDPHKAVAESDLV
                     VSSGPILKHPQPTIQKDWLKPGSFGSAVDFDSYWHPSAMAQMDKIATDDHSQFQYYRS
                     VGYFGQTPDPYADLGEIVAGFKPGRQSDDERTLAINLGLALDDMAVAPEIFRRAKELG
                     LGVWLPL"
BASE COUNT          536 a          544 c          574 g          500 t
ORIGIN      
        1 gcacgctaag acctctttcc tgactgcgta aaataataaa ggcgtcggaa gagttgccca
       61 gcgtgaacag ggcaatcacc aggataaagg ctttgatgcg ccgatccaag ttgttccacg
      121 taaagcgaaa tgttgggacg atggaattcg ctgtttttgt agcttcgaca tcgcgagctc
      181 caatgacaag gatcaagacg gcaagtgctg caggcagtat gctcgccagc acgacccact
      241 ggaatgtagc ttgtgtgagc tgagctttgc ctgcctggtt gatccaaatc aaataggcag
      301 ctatgcccag ccccgtgaat gcccctgctg tatcgctggc tcggtgcaga ccgaaggcaa
      361 agccgcgctg ttctgtggag acacttccgg caagtaaagc atctctaggg gctgtacgta
      421 tgcctttacc aatccgatcg acaaagcgga cacccagaac ccatccccaa ctggtggcga
      481 gatatagaaa tggtttcgcc agggtggaaa gcaagtaacc tgatatcgtc aacggttttc
      541 gtttcccaat gcgatcagag aatacccctg agtaaatttt gactagactg gcggtggtct
      601 ctgcgatacc gtcaatgagg cctataatgg ctgtgcccac acccaatacg ttatatagaa
      661 acaacggtaa caggttgttg agcatttctg aagaaacatc ggtcaggaat gaggctgctg
      721 atagcaccca gacattggta ggcaagcgat ggaggcgtgg tgcgggcata tgggattaga
      781 tctttacggg aataaacgcc tggctttaat ctcggcaacc gcatcttccc ggtagcggta
      841 gaggcgcgcg gggcgacctc ctttgcctcg cttgaactgg gttggttcga tgatgccaga
      901 ttcaagcata cgcctgcgga agttgcgctt atccaatggc tcgccgagaa tgatctcgta
      961 cgttttttgg atttcagaca acgtgaaact ttctggcaag agctcaaacc caacggcggt
     1021 atattcaagt ttataacgca cgcgtcgaat agcataagag atgatctcgg cgtgatcgaa
     1081 ggctagatca ggcaactcat ccaatgggaa ccaactagcc tggctggcat cgctaccacc
     1141 ttcgctgcga acagatgcat ctgctggaat cagagcgaag tatgctacac tgaccacccg
     1201 tccacgcgga tcgcgttctg gatcaccata ggtgtaaagc tgttccaggt aggcttgctc
     1261 aataccagtc tcctcgctta attctcgcgc cgcagcatct tccaaggatt cgtcaacctg
     1321 tacaaatcca ccaggtaacg cccagtagcc agcgaatggc tggtttgcac gtcggatgag
     1381 caatacttga aggcgatttt cacgcagtgt gaagatcaca atgtcgacgg tgagcgaagg
     1441 gcgaggatat ttatctatgg ccatgcaatc gtctctctat aatggcagcc agactcccaa
     1501 tccaagttct ttggcacgcc gaaatatctc aggggctaca gccatatcat ccagcgcaag
     1561 gccgaggttg atagcaaggg tgcgctcatc gtcgctttgg cgaccaggtt tgaaccctgc
     1621 cacgatctca cccaggtcag cgtatggatc tggtgtttgc ccaaagtagc caaccgagcg
     1681 ataatattga aattgggaat gatcatctgt ggcaatttta tccatctgcg ccatggcgga
     1741 tggatgccag taactgtcaa aatctacggc actgccaaag gagcccggtt ttaaccagtc
     1801 tttttgaatg gttggttgtg ggtgcttgag aatcgggccg gaggatacaa ccaaatcgct
     1861 ttctgcgaca gccttgtgcg ggtcgtccac accgataatc tcaaggtcca gctttttcga
     1921 catctcttca acgtaacgtt tctgaacctc tggcacaatg tcatatgcat aggctctctg
     1981 gatggggaaa atggctttta acgcctcaag attggtgcga ccttgcacgc cgcaggctaa
     2041 gatacctgct acactgcttt cagggcgagc aagaaagcgg gcagacaaag cagtagcggc
     2101 tgccgtgcgg taggcggtga tccaggtgca gtccatgact gcgtacggca ggcc
//
LOCUS       DBNHOH010000378         1686 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1175822, whole genome shotgun sequence.
ACCESSION   DBNHOH010000378 DBNHOH010000000
VERSION     DBNHOH010000378.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1175822"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..1116
                     /locus_tag="AC16V7_06725"
     CDS             112..1116
                     /locus_tag="AC16V7_06725"
                     /inference="COORDINATES: protein motif:HMM:NF014646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR6256849.1"
                     /translation="MTSLVQIHTGSEEPLYNIGVVSRMTKISMATLRAWERRYEFPES
                     KRTMGGHRLYSEKDIIRLRWVKERVDEGMQTAQAIHALRHQEQTGQAVLTEEDFRELT
                     PARIERMDAIDKGDREDRRSFSASVQERLLYALLHHNTQQADQLIADALAVLSPEVVI
                     LDIIRSVLNEVGELWQQDKISIANEHLATNYLRQRLLMWMVSGPPPRTQNSIVLACAP
                     DEWHEGSLLILGALLRRRLWPIVYLGQSVPLPDLASFVHDVNPSMVVLNAMTEETAKN
                     LMDWPKLLPDVVSKGRPIVGFGGRVFVEKPEWRLKIAGIYLGNNFEEGILTIEKLLRD
                     "
     gene            complement(1227..1298)
                     /locus_tag="AC16V7_06730"
     tRNA            complement(1227..1298)
                     /locus_tag="AC16V7_06730"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1264..1266),aa:His,seq:gtg)
     gene            complement(1318..>1686)
                     /locus_tag="AC16V7_06735"
     CDS             complement(1318..>1686)
                     /locus_tag="AC16V7_06735"
                     /inference="COORDINATES: protein motif:HMM:NF015113.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HIR6256850.1"
                     /translation="GDMVIGLLLEKYGHLPESLDTSPAPVLVTVFDDKSLLASFTLAA
                     ELRRAGINVNCYPEVAKLSKQFKFADRVGIRLAVVLGPEEQQSGMVTIKNLHSGEQIT
                     ISRSSASSTISHWLEKPASP"
BASE COUNT          490 a          349 c          421 g          426 t
ORIGIN      
        1 tgccaggata aatgtcaata tattgtatag caattgtcga ataatattga caaaaaagac
       61 tttggatgat aaactaaatc tataaaaaat ggacaaaaaa caggagtgtt tatgacttca
      121 cttgtgcaga ttcacacggg gagtgaagaa cctttataca atatcggtgt tgtctcgcga
      181 atgacaaaaa tttcaatggc gaccttgcgt gcctgggaac gcaggtacga atttccagaa
      241 tccaaaagga caatgggcgg gcatcgcttg tactcagaga aggatatcat tcgcttacga
      301 tgggtaaagg agcgcgtcga cgaagggatg caaaccgctc aagcaatcca tgcgctacgc
      361 caccaagaac agacaggcca ggcagttctc actgaggaag acttcagaga actgacgcca
      421 gcccgaattg agcgtatgga tgccatagat aagggtgaca gagaagatag acgaagcttt
      481 tctgcgagcg tccaagagag gttgctgtat gcgttattgc atcataatac ccaacaggca
      541 gaccaattaa ttgctgatgc gctagccgtt ttatctcccg aagttgtcat actggatatc
      601 atcaggtcag ttttgaatga agttggcgag ctgtggcagc aagataaaat cagcatagct
      661 aacgagcatc tagcaacaaa ttatttacgc caacgcttgt taatgtggat ggttagcgga
      721 cctccccccc gaacccagaa ttctattgtc ctggcctgcg cgcctgacga atggcatgaa
      781 ggcagcttgc tcattctagg tgctctcctg agaaggaggt tatggccaat agtgtacctc
      841 ggtcagtcgg ttccgttgcc agatcttgcc agcttcgtac atgatgtgaa tccttcgatg
      901 gtcgtattaa acgcaatgac ggaagaaaca gcaaaaaact taatggattg gcctaagctg
      961 ttgcctgatg tagtttcgaa ggggcgtcct attgttggat ttggcggtcg agtttttgta
     1021 gaaaaacctg agtggcgtct gaaaattgcg ggtatctact tgggtaataa ctttgaggaa
     1081 ggtatattaa cgatagagaa gctattgcga gattaagatg gccgtacatt gggggatatg
     1141 caaaattcgt ttgatgtgga tagaaaaaga aagaattatt ggtttaataa aagggacttt
     1201 tcgaaagaaa agtccctttc agctagggtg cctgatgggg gtcgaaccca caaccacctg
     1261 atccacagtc aggtgctctg ccattgagct acaggcacca cagcaagccg tattctatca
     1321 tggagaggcg ggcttttcaa gccaatggct tatagtagat gatgctgagg atctcgaaat
     1381 ggtgatctgt tctccagaat gaaggttttt gatagtcacc ataccgctct gctgctcttc
     1441 tgggccgagg acaacagcca gccgtatacc aactcgatct gcaaatttaa actgcttgct
     1501 taactttgct acctctggat agcaattcac atttatgcct gcgcgacgaa gctctgccgc
     1561 cagagtgaaa gaggctaaaa gcgatttatc atcgaagaca gtaactaaga ctggtgccgg
     1621 agaggtatct agactttcag gtaaatgccc gtacttttct agaagtagac cgattaccat
     1681 atcccc
//
LOCUS       DBNHOH010000379         2559 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_277864, whole genome shotgun sequence.
ACCESSION   DBNHOH010000379 DBNHOH010000000
VERSION     DBNHOH010000379.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_277864"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..1653)
                     /locus_tag="AC16V7_06740"
     CDS             complement(151..1653)
                     /locus_tag="AC16V7_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HIR6256851.1"
                     /translation="MESKLKQLKVILGEVVDLRNVSQVLGWDQQTYMPPGSSEDRGYH
                     LATIDQLAHVKFTSDEVGKLLDELEPWTAQLDPDSDEARLVKVTRREYDKLIKVPPDM
                     VMGFSQITTAAFSVWQEARAQSNFAMFQPHLEKVVDWRRRYAALFAPYDHVYDPLLDD
                     FEPGMKTADVKAIFDTLRPQQVALIQAISESPQVDNSFLHQSFDEEKQWDFGVEAATK
                     FGYDWDRGRQDKSAHPFTTSFGINDVRITTRFMPNYFSSAFFSTAHETGHALYDLGID
                     PALARTPLAGGTSLAVHESQSRMWENLVGRSLPFWEGFYPRLQEYFPDQLGNVSLESF
                     YKGINRVQPSLIRVEADEATYNLHIMLRLEIEIGLMEGSLDVKDLPEVWNARMQEYLG
                     QTPPNDTQGVLQDVHWSIGYLGYFSTYALGNLVSVQLWERINADIPDLSEQIRRGEFS
                     ALLGWLREKIHRHGAKFEPQELVERITGSKIDPALYMRYLTTKYSQIYGL"
     gene            complement(1732..>2559)
                     /locus_tag="AC16V7_06745"
     CDS             complement(1732..>2559)
                     /locus_tag="AC16V7_06745"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HIR6256852.1"
                     /translation="REIGAYEALIVRSRTKVTAAVLDAAGRLKVIGRAGVGVDNIDLA
                     AASRRGVIVVNAPQSTTLAVAEQTMALMLALARQVPHADAAMKTGQWLKKELLGVELS
                     GKVLGIIGVGNIGAKVAQLAAAFGMSVLGYDPLLPQEVIRQRGAEAVSLQELYARSDM
                     ISLHLPLTPETRGLLSGQSFGQMKRGVRLVCTARGGIIDETALLGALESGQVAGAALD
                     VFAKEPPGLTALVAHPNVIATPHISAQTVEAQVRAAEDIAREVLSALRGESLRWKVV"
BASE COUNT          588 a          733 c          690 g          548 t
ORIGIN      
        1 gagtctacaa gggtagccgg gtaatgtagc atcctggctt taccagatgg gcaaaaaaac
       61 aattgtaaga tgaggaaaag caggcggata gcctgctttt cctcatctac agagattatt
      121 cctataaaat aggggaaaag atacagagga ctacaacccg taaatctggc tgtattttgt
      181 ggtcaggtag cgcatgtaaa gcgccgggtc gatcttcgag ccagtgatcc gctccaccag
      241 ctcctgtggc tcgaacttgg ctccgtggcg gtggattttc tcccgcagcc agcccaaaag
      301 tgcagagaac tccccacgac ggatctgctc gctcaaatcg gggatatcgg cgttgatgcg
      361 ttcccacaat tggacggaaa ctaggttacc cagggcatag gtggagaagt agcccagata
      421 accaatagac cagtgcacat cctggagcac gccctgggtg tcgttagggg gagtctgtcc
      481 caggtactct tgcatgcgcg cattccacac ttcgggcagg tccttgacat ccaggctgcc
      541 ttccatcagg ccgatctcga tctccaagcg cagcatgatg tgcaggttat aggtagcctc
      601 atccgcctca acccgaatca gggaaggctg gacacgattg atgcctttat agaaggattc
      661 caagctcacg ttacctagct ggtcagggaa gtattcctgc aagcgggggt agaagccttc
      721 ccagaagggg agcgagcgac ctaccagatt ctcccacatg cgcgattgcg attcgtgaac
      781 ggcgagcgat gtgccccctg ccagtggtgt gcgcgccagg gcagggtcga tacccagatc
      841 atacagggca tgcccggtct catgggcagt gctgaagaag gcagagctga agtaattagg
      901 catgaaacgc gtggtgatgc gcacgtcgtt gatgccaaag ctggttgtga aaggatgcgc
      961 cgacttgtcc tggcggccgc gatcccagtc gtagccgaat ttcgtagctg cctcgacccc
     1021 gaaatcccat tgcttttctt cgtcaaagga ttggtgaaga aaagaattgt ccacctgggg
     1081 gctttcggag atggcttgaa taagggcaac ttgctgcggg cgcaatgtat caaagatcgc
     1141 cttgacatct gcagtcttca taccgggctc aaagtcatct aacaggggat catagacgtg
     1201 atcgtagggg gcaaataggg cagcgtagcg acgcctccag tccaccacct tttccaggtg
     1261 aggctggaac atggcaaaat tgctctgagc gcgggcttct tgccaaacgc taaaagctgc
     1321 cgtggtaatc tgggaaaacc ccatcaccat gtctggtgga accttgatga gcttgtcata
     1381 ttcgcggcgg gtgaccttga ccagccgggc ttcgtctgag tcaggatcga gctgggcagt
     1441 ccacggctcc aattcgtcca gcagcttacc cacctcatca gacgtaaact taacatgagc
     1501 cagctggtcg attgtcgcca ggtggtagcc gcggtcctcg gaacttccag gaggcatata
     1561 ggtctgctgg tcccatccca acacttggct gacatttctc aggtctacca cttcacccaa
     1621 aatgactttc agttgcttca attttgattc catctgtatt tcacctcgtt aaatttctct
     1681 tcaaatcctc aagaggaata aattaattgc gcgttctgca gaaaaccatc cttaaaccac
     1741 cttccaccgc aatgattcgc ctcgcagcgc agacaacact tcacgggcga tgtcttcggc
     1801 tgcgcgcacc tgagcctcaa ccgtctgggc actaatatgg ggggtagcga tcacgttggg
     1861 atgggctacc agggctgtca gaccgggtgg ttctttggca aacacatcta aagccgcacc
     1921 ggctacctgc ccagactcca gcgcccccaa cagggcggtt tcgtcaatga tgcctccccg
     1981 ggcggtacaa accaggcgca cgccgcgctt catctggccg aacgactgcc cgctaagcag
     2041 ccccctcgtc tcgggggtga gcggcaagtg caggctgatc atatcagagc gagcgtaaag
     2101 ctcctgcaag gacactgctt cagcgccgcg ctgacgaatg acttcctgcg ggagcaaagg
     2161 atcataacct aataccgaca tcccaaacgc ggcggcaagc tgggcaacct tagccccaat
     2221 attacccaca ccgatgatcc ccagcacctt tccagagagc tccacgccga gcagctcttt
     2281 tttgagccac tgaccggttt tcatggctgc gtcggcgtgc ggcacctggc gcgctagggc
     2341 taacatcagt gccatggtct gttcagccac agccagcgtg gtggattggg gcgcattgac
     2401 cacgatcacc ccgcgcctgg aagcagccgc cagatcgata ttatctactc ctacccctgc
     2461 tctcccaatg acttttaggc gtccggcggc atccaacaca gccgcagtca ccttggtacg
     2521 gctgcgcaca atcaacgcct cgtaggcgcc aatctcgcg
//
LOCUS       DBNHOH010000380         2574 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1111257, whole genome shotgun sequence.
ACCESSION   DBNHOH010000380 DBNHOH010000000
VERSION     DBNHOH010000380.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1111257"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="AC16V7_06750"
     CDS             <1..366
                     /locus_tag="AC16V7_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR6256853.1"
                     /translation="IALQQGLSIATPAIHHSDQGIQYAAPAYVQVLEQNGIQISMASV
                     GKPEENGYAERLIRTIKEEEVELSEYRDFAEAYHQIGRFIEDVYLHKRIHSALGYLTP
                     AEFELAWRLALTQPVEGTP"
     gene            complement(421..795)
                     /locus_tag="AC16V7_06755"
     CDS             complement(421..795)
                     /locus_tag="AC16V7_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256854.1"
                     /translation="MKWKTFLIIHALFLAFNGLGTLFIPQIAWGMWGAKVDGTFFVRL
                     LGTLMTANAVLSWFFREVTDAKARRAIVYQQLLAWGLALILFVVGQFDGTFAPIGWAT
                     VVLGGLFTFGWVYFGFIKPNAE"
     gene            complement(1530..2306)
                     /locus_tag="AC16V7_06760"
     CDS             complement(1530..2306)
                     /locus_tag="AC16V7_06760"
                     /inference="COORDINATES: protein motif:HMM:NF044618.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256855.1"
                     /translation="MTSYFLPHFDSTLTAGYFRPGGPFDPHSGTYYQYSQLADLSFKR
                     LGGAFTVPADSHSLKFWISYDIETDWDFAFVEINEVGTDSWTTLADVNGLTTDNTGDS
                     CQSGWVDEIHPFLAHYMDAACNPSGSTGSWNGFTGNSGGWQQVEMDLSAYAGKTVELH
                     ISYATDWSVQNLGVFVDDIELSGYPLEDFEAGSGSWSTSVPAGSTAFNNWVRITGAGI
                     PEGPAIRTPYSVYLGFGLEAIDTAANRATVMDKVMEYLGQ"
     gene            2226..>2574
                     /locus_tag="AC16V7_06765"
     CDS             2226..>2574
                     /locus_tag="AC16V7_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256856.1"
                     /translation="MRVEGPARAEVARGQGAVEVGQEVAGHQEGAGAALVVRAVATLQ
                     GEAHPIEEITGGARHVEWQVGSCIYAPSILVDKRAQVVLEEVVCVITTRGRQPGQILA
                     SCQQLAIFIVHKLP"
BASE COUNT          609 a          729 c          685 g          551 t
ORIGIN      
        1 atagccttgc agcaggggct tagcatcgcc acacctgcca tccaccacag cgatcagggc
       61 atccagtatg ctgccccggc ttatgtgcag gtgctggagc aaaacggcat ccagatcagc
      121 atggcgagcg tgggcaagcc cgaagagaat ggctacgccg aacgtctgat ccgcaccatc
      181 aaggaagagg aggtcgagct gtcggaatat cgtgactttg cagaagccta tcatcagatc
      241 ggtcgtttta ttgaggatgt atacctgcac aaacgcatcc attcagcgtt gggatacctg
      301 accccggctg agttcgagct cgcttggcgg ctcgctctca ctcagcctgt ggagggtacc
      361 ccctaagaaa tcctaaaaaa gtgtccagtt tttcgagacc accacaataa cttcccttgt
      421 ttactccgca ttcggcttga tgaaaccgaa atacacccag ccgaacgtga acagcccacc
      481 caacacgacc gtagcccagc caattggtgc aaaggttcca tcaaattgac caacaacgaa
      541 caggatcagc gccagccccc aggctagaag ttgctgatac acaatagcgc gtcgtgcttt
      601 tgcatcagtt acctcccgga agaaccaact caagaccgca ttggcagtca tcagggttcc
      661 aaggagccgc acgaagaatg tcccatcaac cttcgcaccc cacatccccc aagcaatctg
      721 ggggatgaaa agggttccga gtccattgaa ggccaagaat aaagcgtgaa tgatcaaaaa
      781 agtcttccac ttcatccgat ttgctccttt caattggtga caaattccca attcatagtt
      841 ccacacatgg acacaagccg gtctctgctt cgagacgagc ctaccctatg caaaagctac
      901 atacgcacct ccttgaaacc gatttgcaag ccgccgcccc gatggcacca acgagcgcag
      961 cgagagcgaa gcggtcggtg ccattagtgc atatactgca aattatccgt ataaacagtc
     1021 aattcggtat cttatactga aattggcttt tcataggagt ggttatctta actatcaaat
     1081 atactttaca acatttttct cgtattatca acagcttcag caatgaattc tcaaaacaat
     1141 aacgcccagt gagaactggg cggggggact ctgtgctatg ctccgccctt ccagagcaca
     1201 ggcgcgctgg gtagccagag gcgggaggcg taacgtgctg attagcacta cagacccagg
     1261 cttgctattt ggctgagagc agcttgtggt aatcctcagg cacacacctg tactgcgcga
     1321 aaggtgtagc ctgcctaccc ccttggctat tcgtataggc tttgaggttc tcgtatctta
     1381 gggttcccct tttgcgccct cgcccaagac caaagacttt gaaagatatc ctcagagata
     1441 tttctaatag tatatgtaaa tgagaagggc tcatcccaaa tccgaaattt tggatgagcc
     1501 acctatgatc tgcgctggta tacctgtgct tactgcccca ggtactccat cactttatcc
     1561 atgaccgtag cccggttggc tgccgtgtcg atggcctcca ggccaaagcc taggtagact
     1621 gagtagggcg tgcggattgc cggcccctcg gggataccag ccccggtgat gcgcacccaa
     1681 ttgttgaaag cggtgctgcc ggcggggacg ctggtggacc agctgcccga acccgcctcg
     1741 aaatcttcga gcgggtaacc agatagctcg atgtcgtcga cgaagacgcc caggttctgg
     1801 accgaccaat cggtggcgta gctgatgtgc agttcaaccg tcttgccggc gtaagccgaa
     1861 aggtccatct ctacttgctg ccagccgccg ctgttgccag tgaacccgtt ccaggagccg
     1921 gtgctaccgc tggggttgca ggcggcgtcc atgtaatgcg ccaggaaggg atggatctcg
     1981 tctacccagc cggattggca gctgtcgccg gtgttgtcgg tggtcagacc gttcacatcg
     2041 gccagggtgg tccagctatc cgtgccgact tcgttgatct cgacgaaggc gaagtcccaa
     2101 tcggtttcga tatcgtagga gatccagaac ttcaaggagt gcgagtcagc cgggacggtg
     2161 aaggcgcctc cgagccgctt gaatgacagg tctgcgagct ggctatattg gtagtacgtg
     2221 cctgagtgcg ggtcgaaggg cccgcccggg cggaagtagc ccgcggtcaa ggtgctgtcg
     2281 aagtggggca ggaagtagct ggtcaccagg aaggtgctgg agcagcactg gttgtccgcg
     2341 ccgtcgccac cctgcagggc gaagctcatc ccatcgaaga gatcaccggg ggcgcccgcc
     2401 acgtcgaatg gcaagtcggt agttgcatct atgcccccag tatcctggtc gataaacgcg
     2461 cccaggtagt actggaagaa gtcgtctgcg taataaccac ccgcggtcgc caaccaggcc
     2521 aaatcctggc cagttgccaa cagcttgcca tcttcatagt tcataaactc cctg
//
LOCUS       DBNHOH010000381         5687 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_663168, whole genome shotgun sequence.
ACCESSION   DBNHOH010000381 DBNHOH010000000
VERSION     DBNHOH010000381.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5687)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5687)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5687
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_663168"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..537
                     /locus_tag="AC16V7_06770"
     CDS             55..537
                     /locus_tag="AC16V7_06770"
                     /inference="COORDINATES: protein motif:HMM:NF019632.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HIR6256857.1"
                     /translation="MNKKEIIAAMQNSHELLVEMLNELNTEEMDVPGVVGNWTIKDIL
                     AHLTRWEAELVKLLWQLRQGTQPTTILNQGISLDEVNARWHEEDKDRPLDRILKDFHG
                     VREQTIRRLDHFKDDDLTNPQRYTWLGGRPLWMWVAEDSFEHDLEHMEQIQAWRENRG
                     "
     gene            863..1435
                     /locus_tag="AC16V7_06775"
     CDS             863..1435
                     /locus_tag="AC16V7_06775"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009792604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HIR6256858.1"
                     /translation="MKHHTGSIEVITGSMFCGKTDELIRRLRRATIARQRVQVFKPVI
                     DDRYSVEKVTSHAGSEYAAQPIQHAGDIPSLIEPETTVVGIDEAQFFDSDIVPIVQSL
                     ADRGIRVLIAGLDTDFRGEPFGPMPLLMAAAENVDKLHAICMMCGEAASRTQRLVNGK
                     PAHYNDPVVIVGASELYEARCREHHDVPRN"
     gene            1457..2014
                     /gene="hpt"
                     /locus_tag="AC16V7_06780"
     CDS             1457..2014
                     /gene="hpt"
                     /locus_tag="AC16V7_06780"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIR6256859.1"
                     /translation="MQGYKEFLDEILITEEALQNRIAELGAQISKDYEGKQPHLICIL
                     RGGVMFLSDLIRHLTVPNTIDFMAISSYGVGARQSTGKVRITLDLQEDIIERDVLLVE
                     DIVDSGYTIATVLEFLKTRHPSSLRVCTLLNKAERREIQVPIDYCGFVIPDKFVFGYG
                     LDLDELYRNLPFIGTVNPERYHPPV"
     gene            2065..3681
                     /locus_tag="AC16V7_06785"
     CDS             2065..3681
                     /locus_tag="AC16V7_06785"
                     /inference="COORDINATES: protein motif:HMM:NF013871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA nucleotidyltransferase/poly(A)
                     polymerase-related protein"
                     /protein_id="HIR6256860.1"
                     /translation="MGRWVARLGERIIGQGGTPEQALWAARAARSKENPQIDYIPTVQ
                     PLVFSARLEPIRSLMPVDMPAYLVGGAVRDALLMRTTHDLDFVLPSGALKAGRAVANA
                     LRGAYYPLDAVRQTARVILYAPDGTREFLDFAVIRGEDLESDLRARDFTINAMAIDLR
                     HPTQLLDPLGGAADLLARQLRACSPRAFFDDPVRILRGVRLAAELNLKIQPETLKIMR
                     QSVGELERVSSERKRDELFRILDGPQPAKALRALEMLGALVYVLPELVSLKNVMLAPP
                     VDKNVWTHTLDVVRNLATLLDVLDLKYKPDTAANLSMGLVSMRLGRYREQLAEHLKSS
                     ITPERPLRSLLFLAALFHEVAQSQIPQVDESAGDMIDNDIAGAQMVEERGEALHLSND
                     EIARLKIIVRFHRRTILLAQEEDPPSRRAIYRFFRDVGDVGVEISLLSLANTLATYGP
                     APHQDMWARHINTVRMLYEAWWEHHQEYISPPALLGGYDLIDELRLKPGLHIGQILEA
                     IKEAQATGQVTDRDGALSLARSWLAEHSID"
     gene            complement(3678..3872)
                     /locus_tag="AC16V7_06790"
     CDS             complement(3678..3872)
                     /locus_tag="AC16V7_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256861.1"
                     /translation="MKRLRANPRVLNLRLVVADTCCPACQQVEGTYAKDQVPKLPVEG
                     CSHSLGCRCFYEPMLSEIYP"
     gene            complement(4085..4675)
                     /locus_tag="AC16V7_06795"
     CDS             complement(4085..4675)
                     /locus_tag="AC16V7_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6256862.1"
                     /translation="MNNAALTRVLVIDDDAAMTEMLRLILEPNAFDVKVSHSGREAIE
                     SAKHLSPEVIILDLSLEDMDGGEVCKQIRSFSQAPILVLSAVSRPGMVAKALDEGADD
                     FLLKPMTSSVLIAHLKRLVWRARAEENANHRTDKSSQKAQPSTGSTLHRNSEPRRGKD
                     IRALHAAKYPSQPKDDSTLARNKAQRRISTDKPKKV"
     gene            4865..>5687
                     /locus_tag="AC16V7_06800"
     CDS             4865..>5687
                     /locus_tag="AC16V7_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HIR6256863.1"
                     /translation="MSSQIHCPQCGQPTESSSRTCEKCGVVLAIAAVLAEREILPHRI
                     DGSLAVTPEILVPRLGEYLVEKGKLTRPNLERALDYQKKKSRSGKTLLLGQALRELEL
                     ITAEELDQAITEQILNLQVALRRSNQELEARVRERTIELERALEMLGELNQLKANFIA
                     NVSHELRTPLTHIKGYLGVMATGELGPISVDQADALRVMQQAEARLERLIEDLIQFAL
                     ISRGQLTLSLSHANIAEMVENVVNQVQQKANLAGINLETDISEGLPLAVCDNDKIA"
BASE COUNT         1371 a         1360 c         1519 g         1437 t
ORIGIN      
        1 caccttacga acatttggca gcaatccgcc agtttgctga ggagatgggc ttgcatgaat
       61 aagaaagaaa taatcgctgc tatgcaaaat agccacgagc ttttggtaga aatgcttaat
      121 gagctgaaca cagaagagat ggacgtgcca ggtgtagttg gcaactggac gatcaaagat
      181 attttggcgc atcttacccg ttgggaagca gaactagtaa agctgttatg gcaattacgt
      241 caaggtaccc agcccacaac catcctcaac caaggcattt cccttgacga agttaatgct
      301 cgctggcatg aagaggataa agatcggcca ttggatcgca tactgaaaga ctttcatggt
      361 gttcgcgagc agactatccg ccgcttggat catttcaagg atgatgatct gactaatccg
      421 cagcggtaca cctggcttgg aggtcggcct ttgtggatgt gggtggcgga agatagtttt
      481 gaacacgatc ttgagcacat ggagcagatc caggcttggc gagaaaatag gggttagtag
      541 aggcttcctg gttggagaga tatattgact ttggtcaggc ttgattctcc cagagtctct
      601 atttttataa tgatatgtag ctcacagggc aattgcatat gtcctgaggg gcatgcgtaa
      661 tcccgcaaaa agagtgtttg gggtgttttg gcgggggcat acccccacca aaacacccca
      721 ttttctctcc tcagttgtgg gaaatcgccc aaatgcgatt accctactca atccccagct
      781 cctttcagca aacatctttt gggtgtggta gaatccgtca ccagatgtca ttggtaataa
      841 tccaaatcat ccgtggggta atatgaaaca tcatactggc tcgattgagg tgattaccgg
      901 ttccatgttt tgcggtaaaa ccgatgagtt gattcgtcgg ttgcgcaggg caaccattgc
      961 ccggcaaaga gtccaggtct ttaagccagt tattgatgac cggtattccg ttgaaaaagt
     1021 tacctcgcat gcaggcagtg agtatgctgc tcagcccatc cagcatgcgg gtgatattcc
     1081 atcattaatc gagccagaaa ccactgtggt tggcatcgat gaagcgcaat tctttgatag
     1141 tgatattgtc cccattgtgc aatccctggc agaccggggg attcgtgttc tcattgctgg
     1201 attagacacg gattttagag gcgagccttt tggcccaatg ccattgctta tggcggcggc
     1261 agagaacgtg gataaactcc acgcgatatg tatgatgtgc ggtgaagcgg ctagtcgcac
     1321 ccaacgtctg gtaaatggca agcctgcaca ctataatgac ccagtggtga ttgttggtgc
     1381 ttcggaattg tacgaagctc gatgtcgtga gcatcatgat gttccaagga actaagcttg
     1441 ggatgatggg catcttatgc agggctataa agaattttta gatgagatat taatcactga
     1501 ggaagcacta cagaaccgca ttgcggagct cggggctcaa atcagtaaag attacgaggg
     1561 gaagcagccg cacttgatct gcattcttcg cgggggtgtc atgtttttat ctgacctgat
     1621 acgccacctc accgttccga atacaatcga ctttatggcg atttcatcat atggggtggg
     1681 ggcgcgtcaa tctaccggaa aagtgcgcat aaccctcgat ttacaagagg acatcatcga
     1741 acgcgatgtg ctgttggtgg aagacattgt agatagcggc tacaccatcg ccacagtact
     1801 tgagtttctt aaaacacgcc acccgagcag tttgcgagtc tgtacattgc tgaacaaggc
     1861 cgagcgccgg gaaatccagg tgccaatcga ttattgtgga tttgttattc cggataaatt
     1921 tgtgtttggt tatgggttag atttggatga gctgtaccgt aatttgccgt tcattggcac
     1981 ggtgaatcct gagcgttacc atccgcctgt gtaatcaaaa tgccgcgcca gccgaagtcc
     2041 cctccgggta atcatgatcc ctatatgggt cgttgggttg cccgcttggg tgagcgtatc
     2101 atcgggcagg gaggtactcc cgagcaagcg ttatgggcgg caagagcggc ccgtagcaaa
     2161 gagaatcccc agatagatta catacccaca gtgcaaccat tagtattctc cgcgcgatta
     2221 gagccaatcc gtagccttat gcccgttgat atgccggcat atctggttgg cggcgcagtg
     2281 cgggatgccc tcctgatgcg cacaacccac gacctggact ttgttttacc atccggcgct
     2341 ctgaaagcag ggcgcgcggt ggcgaatgcc ttacggggcg cttattatcc cttggatgcg
     2401 gtgcgccaga ctgcgcgagt aatcctgtat gccccagatg ggacgcggga gtttttggat
     2461 tttgctgtaa ttcgcggaga ggatttagag agcgatttgc gcgctcggga ttttaccatt
     2521 aatgccatgg ctattgactt gcgccacccc acgcaattgc tagacccgct cggcggtgcg
     2581 gcggatttac ttgcccgcca attgcgtgcc tgctctcctc gggctttttt tgacgatcca
     2641 gtgcgtatat tacgcggtgt acgcttggct gccgagttga atctgaaaat ccagcccgag
     2701 acgttgaaaa taatgcgcca atccgtgggg gagttggagc gagtctcctc ggaacgaaaa
     2761 cgagatgagt tgttccgcat ccttgatggc ccgcagccag ccaaggcact gcgcgcactg
     2821 gaaatgctcg gcgcgctggt ctacgtgcta cctgagttgg tgagcctgaa aaacgtaatg
     2881 ctagcaccgc ctgtagataa aaatgtgtgg acacacactt tggatgtggt gcgaaacctg
     2941 gcgacccttc tcgatgttct tgatctgaaa tacaaacctg ataccgccgc taacctatcg
     3001 atgggtttgg tatctatgcg tcttgggcgt taccgagagc aacttgcgga gcatctgaaa
     3061 agctccatta cgccagagcg tcccctgcgg tctttgctct ttctggcagc gcttttccat
     3121 gaggtagccc agtctcaaat cccccaggtg gacgagagtg cgggcgatat gattgacaac
     3181 gatatcgcgg gagctcagat ggtcgaagaa cggggagagg cactccattt gagtaatgat
     3241 gagattgcac gtctaaagat aattgtacgc tttcacagga ggacgatcct gctggcacag
     3301 gaagaagacc ccccatcccg gcgagctatt taccgcttct tcagagatgt gggtgatgtc
     3361 ggcgtggaaa tcagcctgct atcccttgcg aacaccctgg caacctatgg cccagctccg
     3421 caccaggata tgtgggcgcg tcacatcaat accgtccgca tgttgtatga agcctggtgg
     3481 gagcatcatc aagaatacat ttctccccct gctctgttag gtggctatga tctaatagat
     3541 gagcttcgcc tcaaacccgg cttgcatatc gggcaaatat tagaggcgat aaaggaagcg
     3601 caagccacag gtcaggtgac cgatcgtgat ggtgcgctaa gtctggctcg ctcctggttg
     3661 gctgaacaca gtatcgatta ggggtatatc tcagagagca tcggctcgta gaagcaacga
     3721 caacccagcg agtgtgagca accctcgacc ggtagcttgg gtacttgatc tttggcgtag
     3781 gttccctcca cctgctgaca agcaggacag caggtgtcgg cgactaccag gcgcaggtta
     3841 agtacccttg gattggcgcg cagtctcttc aaagccgcgg atattttaga gaattcactc
     3901 agatctgctt gacaattact gcaataagca gccgaatctg gagattgggc attgcattga
     3961 gaacagattt gcacgtcact gcctccgtga aaattttcgc taattgtaat cgaaaataag
     4021 gcagttagat atgtcacccc aaacactctc ttcagtggtg ggaaatcgcc ctaatacgga
     4081 tgccctacac ctttttaggc ttatctgtcg agatccttct ctgggcttta tttcgggcta
     4141 gagtggaatc atcttttggc tgggatggat atttggcagc atgtaaggcg cgaatgtctt
     4201 tccccctgcg aggctccgag ttgcggtgca acgtgcttcc agtagagggt tgtgctttct
     4261 ggctgctttt gtcagttcgg tggttggcat tttcttcagc tcgagcacgc cagaccagcc
     4321 ttttcagatg tgcgatcaag acgctactgg tcatgggctt gagcagaaaa tcgtcggcgc
     4381 cttcatccag tgccttggcg accatccccg gtctgctgac tgctgaaagt accaggatag
     4441 gcgcctggct aaaggagcgt atctgcttgc agacttcccc cccatccata tcttccaggg
     4501 atagatccaa gatgatcacc tctgggctca ggtgcttggc tgattcgatg gcttctcgtc
     4561 ccgaatggga tactttaaca tcaaatgcat ttggctctag gattaaccgc aacatttctg
     4621 tcatggcggc atcatcgtcg atcaccaata ctctggttaa tgcagcgtta ttcacctaga
     4681 cggttttact cctcaaatga acgaatcggg atcaggaaaa aattctcctg attgcttccc
     4741 ggctgtctgc tccaattatg cctgaaaaca gcggtgccac accttacaat cacttaacag
     4801 gtcggtctgc gaaaatgctt ctgacttgcg ccctggaaga ttcggagata tgattatatc
     4861 ccagatgagc tcacaaatac actgtcctca gtgcggtcag ccgactgaat cttcttcgcg
     4921 tacttgtgaa aaatgtggag tagtcctggc aattgccgcg gtgttggccg aacgcgagat
     4981 tctgcctcac cggatagatg gtagcttagc ggtaaccccg gaaatcctgg tgccacgtct
     5041 cggtgaatat ttggttgaaa aggggaagtt gacacgtccc aatctagaac gtgccctgga
     5101 ttatcaaaag aagaaatccc gttctggaaa gacgctatta ttgggacaag ccctgcgcga
     5161 gttagaattg atcacagccg aggaattaga tcaggcgatt actgagcaga ttcttaattt
     5221 gcaagtggcc ttaagacgct ccaatcagga gctggaagcc cgcgtgcgcg agcgcacaat
     5281 cgaattggag cgggcgctcg aaatgctggg ggagctcaac cagttgaagg ctaatttcat
     5341 tgccaacgta tctcacgaat tgcgaactcc cttgactcat atcaaaggat atctcggagt
     5401 tatggctact ggtgagttgg gaccgatttc cgtcgaccaa gctgatgcct tgcgcgtaat
     5461 gcagcaagct gaagcacgtt tggagagatt gatcgaagat ttaatccaat ttgcgctcat
     5521 atcgcgcggt cagcttacct tgagcctgtc acatgcgaac attgccgaaa tggttgagaa
     5581 tgtggtaaac caagtgcagc aaaaggctaa tttggctggc atcaatttgg agacggatat
     5641 ctctgaaggt ttaccgctcg cggtctgcga caatgacaaa atagcct
//
LOCUS       DBNHOH010000382         3141 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_791133, whole genome shotgun sequence.
ACCESSION   DBNHOH010000382 DBNHOH010000000
VERSION     DBNHOH010000382.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_791133"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..2708)
                     /locus_tag="AC16V7_06805"
     CDS             complement(183..2708)
                     /locus_tag="AC16V7_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6256864.1"
                     /translation="MEIDLELYRREVRVSVRPRVRISVIDVAPERAQRTICFVHGYGG
                     RATQWKYQLNKFSDTNRVIAMDLRGHGRSDKPPGEYTMPSLLADLDAVLNALDVDDKI
                     TLVGHSFGGAIASEYANAHPGRVDRLVLIASTGEYRLNPFYRFLLSLPNTIHPVVKPF
                     TNKWLGAPPHVMKSLYEHTLSTWVGWSMFRGLNLPVLVIRGHQDIVFEKPLFEEVVRT
                     IPAAEDVDVGASGHLVMLERRDAVNRAIDRFLETSPRTWREAGAAPGETDGSTLVRER
                     PWLPYYDDGVPYGVAIPRVPLQSLLTSAVRRFPSHTALIFEGKRISYLRLNREINCFA
                     NALRAIGVEKGDRVMLLLPNLPQMIIAFFGTMKAGGVAVFTLPTTTADELLRQIQESG
                     ARTLVTLVEFGALLQEMQEKFTATDLPLQHIILSRGGDYLDTTKRFLVKLSGKGNQPN
                     LNDGFLGSKVQPWSSLLSGKSPEAPEVPITPNDLAVIIYTGGTTDVPKGVMLSHQNLV
                     ANALQTRHWIPDASEGRERFLCALPFSHSYGLTTALNVPIALGATLILKPRFEINDVL
                     KTIKQYRPTIFPGVPQMYVAIKDYPGVRRYGVSSIKACISGSAPLPVEVQEAFEKLTR
                     GRLVEGYGLTEASPVTHGNPLKGLRKVGSIGVPFPSTEARVVDLRNGKKEVRVGQIGE
                     LAVRGPQVMLGYWQNEEETRRVLTTDGWLLTGDVAQMDSDGYFRIIARKAEMWYPSKP
                     GEPAFPRDVEEVLFEVPQVKEAAVVAVAGQPIAFIIARSERPTADALVAYCKRRLPPE
                     IVPRLVIFVDDFPRSFIGKVLRRELAKRFEERTKEEGEQTIDE"
     gene            complement(2876..>3141)
                     /locus_tag="AC16V7_06810"
     CDS             complement(2876..>3141)
                     /locus_tag="AC16V7_06810"
                     /inference="COORDINATES: protein motif:HMM:NF013508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256865.1"
                     /translation="SASGDGWTCDEEGSVVTCSRPSLPAGDAPLITLTVTAPPDPGTI
                     TNSAVVSAGTEDPNVANNSDNVQTTVNPVPGIIRRLYLPLITR"
BASE COUNT          731 a          848 c          837 g          725 t
ORIGIN      
        1 caaggcatag gcagagtaac cgttggcagc cagagaagga gcaatttcag tccaatcata
       61 aagcgaggca gccatgccat gtattaagat gaccggctga cctcttccga gtgagatatc
      121 gttggcgtga aattcgttca ttctaattgt tgaaatacct gagaccaaat gatgggctaa
      181 tcttattcgt cgattgtttg ctccccttct tccttcgtcc tctcctcaaa gcgctttgcc
      241 agctcccgtc gtaacacttt tccgatgaaa gatcttggaa aatcgtcgac aaatatgacc
      301 aggcgcggca ctatttctgg tggtaagcgg cgcttgcaat aggcaaccag cgcatcagct
      361 gtggggcgtt ctgaacgggc aatgataaag gcaatcggct gcccggcaac cgctacaacg
      421 gcagcttcct ttacctgggg tacttcaaag agcacttcct ccacatctcg cgggaaggca
      481 ggctctccag gctttgaagg ataccacatc tccgcctttc tagcgataat acggaaatac
      541 ccatcgctgt ccatctgagc cacatcaccg gtcagcaacc agccgtcggt ggttagcaca
      601 cggcgcgtct cttcctcatt ttgccagtag ccaagcatta cttgtggtcc gcgtactgcc
      661 agctcgccaa tctgaccaac acgaacctct ttcttcccat tcctcaggtc aaccacgcgg
      721 gcttctgtgg acgggaaagg aacgccgatg ctacctacct tgcgcaagcc tttaagcggg
      781 ttgccatgtg ttacaggcga ggcctccgtt aatccataac cttcaactag ccggccacgc
      841 gtcagtttct cgaaagcctc ctgcacctct accggtagag gtgcagatcc gctaatacag
      901 gccttgattg agctcacacc atagcggcgt acacctggat agtctttgat cgccacatac
      961 atctgcggga caccagggaa aatcgttggg cgatattgct tgatcgtttt caacacatcg
     1021 ttaatttcaa acctgggttt caagatcagc gtggcaccca aagcgatggg aacattcagg
     1081 gcagtggtca gcccatagct gtgcgagaag ggtagcgcgc agagaaaacg ctcacgcccc
     1141 tcgctcgcat caggtatcca atgacgggtt tgcaaggcgt ttgccactag gttttgatgc
     1201 gaaagcatca ctcccttggg cacatcggtg gtgccgccgg tgtaaataat gacagccaga
     1261 tcgttggggg ttatgggtac ttctggagct tctgggctct tgccagacaa caagctactc
     1321 cagggttgca ccttgcttcc caaaaagccg tcattcaaat tgggttggtt tccctttcca
     1381 gacaacttaa ccagaaaacg tttggttgta tccaggtaat cgccgccgcg agacaggatg
     1441 atgtgctgaa gtggaagatc tgtggcagtg aatttttcct gcatctcctg aagcagcgcg
     1501 ccaaactcga ccagcgttac caaggtgcgg gcgccacttt cctggatttg ccgcagcagc
     1561 tcatcggctg tggtggtggg cagggtgaag accgccacgc cacctgcctt catcgtgccg
     1621 aagaaggcga tgatcatctg gggtaagttt ggcaagagca gcatcacccg atcgcctttt
     1681 tcaacgccga tggcacgtag agcgtttgca aagcaattga tctcccgatt caggcgcaga
     1741 tagcttatcc gcttgccctc aaatatcaat gccgtgtggg atggaaagcg gcgtacagcg
     1801 gaggtaagca aggattgaag cgggacgcgc ggaatggcga cgccatatgg cacaccatca
     1861 tcgtaatacg gcaaccaggg acgctccctt accagggtgc ttccatctgt ttctccaggc
     1921 gcggcccccg cttcgcgcca ggtgcgcggg ctggtttcta gaaatcgatc gatagcccgg
     1981 ttgacggcgt ctctacgctc cagcatcacc aggtggcccg aagcacccac atcgacatcc
     2041 tcagcagccg gaatggtgcg cacaacttct tcgaagagag gtttctcaaa gacaatatct
     2101 tgatggccgc ggatgacaag aacaggcaga ttcaatcctc tgaacatact ccaacccacc
     2161 caggtggata aggtatgctc atataaggat ttcatcacat ggggaggtgc cccgagccat
     2221 ttgttggtaa agggtttcac cacagggtgg atggtgttcg gcaagctgag taaaaaacgg
     2281 tagaaggggt taagacggta ctcgccagtt gatgcaatca gcaccaggcg atcaacgcgc
     2341 cctggatgag cgttggcgta ctcgctggcg attgctccgc caaaagaatg gccgaccagc
     2401 gtaatcttat catctacatc taaagcgttt agcaccgcgt ctaaatctgc tagcaggcta
     2461 ggcatggtat attcgcctgg tggcttatca gagcgcccgt ggccgcgcaa atccatggca
     2521 atgacccggt ttgtgtcaga aaatttattt agctggtact tccactgggt tgccctaccc
     2581 ccgtatccgt gaacaaaaca aattgtgcgt tgcgcgcgct cgggagcgac atcaattacc
     2641 gagatacgca ctcgcgggcg cacagagaca cgcacctctc gacggtagag ctctagatca
     2701 atttccatgc ttggaagtct acaacattta aagggaatcg taaagcgttt gtcgtgttga
     2761 aaaaaacaga cgatccgctg gttgcagcca ccggatcgtc tgtttttggt tacttatcgt
     2821 ccatcaggta ttttgcacca tcaggtgatt tgtcctgctt gccatatccc cgaggttatc
     2881 tggtaatcag cgggagatag agccggcgaa tgatgccagg aaccgggttt acagtcgtct
     2941 gtacattgtc ggagttgttg gcgacgttgg ggtcttcggt acctgcgctg accacagctg
     3001 agttggtgat cgttcctggg tctggcgggg cagtgacagt cagggtgatg agcggggcat
     3061 ccccagccgg tagagacggg cgggagcagg taaccacact accctcttca tcacatgtcc
     3121 agccgtcgcc cgaagcgcta t
//
LOCUS       DBNHOH010000383         4318 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1752834, whole genome shotgun sequence.
ACCESSION   DBNHOH010000383 DBNHOH010000000
VERSION     DBNHOH010000383.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4318)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1752834"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="AC16V7_06815"
     CDS             <1..698
                     /locus_tag="AC16V7_06815"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6256866.1"
                     /translation="LLHSAGGSLLSWPAEIRRLAGYRIYALDLPGHGKSGGQGYQSIS
                     SYADVVMEWLDAVGLSRAVFVGHSMGSAIVQMLALDHPGHVLGLVLVGSAASLPVNPM
                     LINESANQTTFNKAVEKVVSWSFSPQTPQNIKTLVAKRLAETRPSVLHGDFLACDTFN
                     VTNRIHEIHQPTLVICGSQDKMTPVRNAYFLADNLPAARVEIIPGAGHMVMLERPQEV
                     AASLAQFFRQIPY"
     gene            781..852
                     /locus_tag="AC16V7_06820"
     tRNA            781..852
                     /locus_tag="AC16V7_06820"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:813..815,aa:Met,seq:cat)
     gene            complement(1185..1637)
                     /locus_tag="AC16V7_06825"
     CDS             complement(1185..1637)
                     /locus_tag="AC16V7_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256867.1"
                     /translation="MSKKPINIDEREMNIFRNICTTLYIITLFSLIGMQLYRQFILHQ
                     SQEEWNDIALLIAFNVVVLLGSVLYVSGTINPKKIKFRYLIAGYLGFILFGFLFTIFK
                     YTVLLGQNIGLTQVLDYLLIVASISGLLALTWGLLAFLGSKRIEKLIE"
     gene            complement(1630..1851)
                     /locus_tag="AC16V7_06830"
     CDS             complement(1630..1851)
                     /locus_tag="AC16V7_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR6256868.1"
                     /translation="MTMKAQVNMKLKLARVEKQLSQQELADIVEVTRQTIGLIEKGNY
                     NPTLKLCVKIAKALDRTLDQLFWEDEKGE"
     gene            complement(2227..4095)
                     /locus_tag="AC16V7_06835"
     CDS             complement(2227..4095)
                     /locus_tag="AC16V7_06835"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6256869.1"
                     /translation="MKNIQHIMRLASLLIALLFSMSSCSTKASVNSDNTFYSPDKAAA
                     DMEVYARFEAELESIRQKSMVPGFSAAIVKDQQVTWAAGFGYADLEKQVEATPSTAYP
                     LASLTKPFAAAIIMQLVEDGTLSLDDPITDYGVDFSSDGVIELRHVLSHTSHGMPGEE
                     YRYNGDRFADLGYLVRNASGRSFRELLNARILEPLEMTNTAPNPISMGDDFLDVFRIW
                     MDADNTRVYRATAKPYRLDQEYKLVKSGCPDFFTPASGLISTVLDIAKFDIAMDQNIL
                     LEQETKVQMFTPTTSNNGSNLPYGIGWFTQEYKDQPLIWHYGWQPSCGSSLILKIPDE
                     EITFIVLANSDNLSRPYRLGSGEVLAIDSILALAFYKTFVFEPQYSLVVPQIDWEADE
                     SDLVAQLQEVENENVTEILQRELLSYRKLFHSVGRVDQAEKLLNVYEKVFMNTEARLE
                     NYAELLELGELPWIYLPLPRMRHVTILGFFVLIVLSVFTLWPIDYAIGRLRTRKSGLS
                     RSVDKNKKAARNARAAAYLAVITCLVVVFLFVSYISSYPNGGLLEWKNGTMLVKTLIG
                     FANLSVVFSFLLAGFTIQAWRKGYWSAGWRIHYTLTTIAILCNSYLWQQLDLLGWV"
BASE COUNT         1180 a         1016 c          931 g         1191 t
ORIGIN      
        1 tcctgctgca cagtgcaggc gggtcgctcc tttcttggcc tgcggagatc cgtcgtctgg
       61 ctggatatcg catctacgcc ctagacctgc ctggtcatgg gaaatcgggc gggcagggat
      121 atcaatccat ctccagctat gctgatgttg ttatggaatg gttggatgca gtgggcttat
      181 ctcgcgcggt cttcgttggg cattctatgg gcagcgccat tgtccagatg ttggcattag
      241 accatcctgg ccatgtgctt ggattagtat tggtcggcag cgcagccagt ttgcccgtca
      301 acccaatgtt aatcaatgag tctgctaacc aaaccacctt caataaggcg gtggaaaaag
      361 ttgtttcctg gtctttcagc ccccaaacgc cacaaaacat caaaaccttg gtagccaaac
      421 gtttggctga aacccgccca agcgtgctgc atggtgattt ccttgcgtgt gacaccttca
      481 acgtgaccaa ccgtattcac gaaattcatc agcccacatt ggtgatttgc ggctcgcagg
      541 acaaaatgac ccccgtgcga aacgcgtact tccttgcaga caaccttcct gcggctcgcg
      601 tggaaatcat accaggggct ggacacatgg tcatgttaga gaggcctcaa gaagttgctg
      661 cctccctggc gcaatttttt cgccagatcc catactaatt ctcaaactga gagcattttc
      721 gcttgacgcc catgggtggg cgtgctataa tgtgggccgc ccagtaaacg ggcaaaacga
      781 tgcggggtgg agcagtggca gctcgttggg ctcataaccc aaaggtcctt ggttcgaatc
      841 caagccccgc tactataaga aagcaccttt ttcaaggtgc ttttttgcgt attaggtatg
      901 tggttaagta acggaacctg aaatccgaag actatgagcc cgtcaaagtc cttgtaaagg
      961 ctccataatt aattgaggct cgggttttag agttaggatc ttctcgactg gaggccgttg
     1021 gtgtggatga tctgcccttt tttgatgctg gatttttggc cttaccaatt ctttcatctc
     1081 gaatcatgat cataccctac atcccaaaaa atatctaatc aagcgtcttt tccgtcgttc
     1141 tgtcgatcga caactcctga ctacaaacca tatgagatat attttcactc tatcagcttt
     1201 tcaattcttt tgcttccgag aaacgcgagg agaccccacg taagcgcaag gagcccacta
     1261 atagacgcga ctatgagtaa atagtccaat acttgcgtca ggccaatgtt ctgccccagt
     1321 agaacagtgt acttaaagat cgtaaacagg aaaccaaata ggataaagcc gagataaccg
     1381 gcgatgagat agcgaaattt gattttcttc gggttgatgg ttccactaac atacaaaact
     1441 gaccccaaca aaacgacaac attaaatgca atcagaagag cgatatcgtt ccattcttct
     1501 tgtgattgat gcaggataaa ttgtctgtac aattgcatgc caatcaacga aaacaaagtg
     1561 ataatataca acgtggtaca gatattccga aagatgttca tttcgcgttc atcaatattg
     1621 atgggtttct tactcacctt tttcatcctc ccaaaacagt tggtctagcg ttctatccaa
     1681 tgcttttgca attttgacac aaagtttgag cgtagggtta tagtttcctt tttcaatgag
     1741 cccgatggtt tgacgggtca cctcgacgat gtcagcaagt tcttgctggg agagttgttt
     1801 ttcaactctg gccagtttga gcttcatgtt aacttgtgcc ttcatggtca taatatacta
     1861 cataaattgc ataatgtcaa gtatatattt tattaagcaa tttaattatt acaatttatg
     1921 aagtagtttc aagcttatac gcttccccgt tctgcgcgga aaagtactcg aataagagat
     1981 agggtagata tcttacgagt ggctgaaaaa tgaaggattg acagtgaaat ttcctcgggc
     2041 aataaatgca tcatttgatg tacgtcaggc acatatactc ccaaccaccc gggtcatcca
     2101 cctcagacta gcgccccaac aggctcagat agattgaact cgatcatatg agatggctgc
     2161 tcattaggtt gttcactcct gggatccatg cgaattctcc ttgtccattc catccaaagc
     2221 cttgaattag acccatccca acaaatctaa ttgttgccat aaataactgt tgcatagtat
     2281 agctatggtt gtgagcgtat agtggattcg ccatccggcg ctccaatagc cttttctcca
     2341 cgcctggatt gtgaaacccg ccagcaagaa cgaaaaaaca acgcttaaat tcgcgaaccc
     2401 gatcagtgtt ttaacaagca ttgtcccatt cttccactcc aataaccctc catttggata
     2461 gctggaaatg tagctgacaa agagaaatac gacaactagg catgtgatta cagcaagata
     2521 tgccgcagct cgagcgtttc tcgctgcttt tttatttttg tcaactgatc tggataagcc
     2581 agactttcga gttcgtaatc gaccaattgc ataatctata ggccataggg tgaacaccga
     2641 gagaacaatc agcacgaaga aaccaagaat ggtgacgtga cgcattctcg gcaatggaag
     2701 atatatccag ggtaactcac caagctcaag taattcagcg tagttttcca ggcgcgcctc
     2761 ggtgttcata aatacctttt cataaacatt cagtaacttt tcagcttgat ccactcgacc
     2821 aacgctgtga aaaagtttcc gatatgataa tagctccctc tggagaattt cagtcacatt
     2881 ttcattttca acttcctgca attgggctac gagatcactc tcatcagcct cccaatctat
     2941 ttgaggcacc accaggctat attggggttc gaagacgaat gtcttataga acgctaatgc
     3001 caggatcgaa tcgattgcca atacttcccc cgaccccaaa cgatatgggc ggctcaagtt
     3061 atctgagttt gccagaacga taaaggttat ctcttcatca ggtattttaa ggatcaaaga
     3121 tgatccacaa gaaggctgcc agccgtaatg ccagattaag ggttgatctt tgtattcttg
     3181 tgtaaaccag ccgataccat agggcaaatt gcttccgttg tttgatgtgg ttggggtgaa
     3241 catctgcacc ttggtttcct gctcgagcag gatattttga tccatggcga tatcaaattt
     3301 agctatatca aggacagttg aaatgagacc agaagctggc gtgaagaaat cagggcaacc
     3361 gcttttcact agcttgtatt cttgatccaa tcgatagggc ttggccgttg ctcgatatac
     3421 cctggtgttg tctgcatcca tccaaatacg gaacacatcc agaaaatcat cacccatgct
     3481 gatgggattg ggagcagtgt tggtcatttc gagaggctcc agaatccgag catttaacaa
     3541 ctccctaaac gaccttccag aagcattcct aaccaaataa cctagatcag caaaacgatc
     3601 tccattgtag cgatattctt cacccggcat tccgtgggag gtatgcgaca agacatgcct
     3661 caactcgata acaccatcac tggaaaaatc taccccatag tcagttatcg ggtcatcaag
     3721 actaagcgtt ccgtcttcaa cgagctgcat gataatcgct gcagcaaaag gttttgtaag
     3781 agatgctagc ggataagcag tgctgggggt cgcttcaacc tgtttctcga gatcagcata
     3841 gccaaatcct gctgcccaag taacttgctg atctttgacg atagcagctg aaaagccggg
     3901 aaccatcgat ttttggcgga tgctttccaa ctcagcttcg aatcttgcgt agacttccat
     3961 atctgctgca gccttgtctg gcgaatagaa tgtattatcc gaattgacac tcgcttttgt
     4021 tgagcagctg ctcatcgaga ataacagtgc aatgagcagg ctagctagcc tcatgatatg
     4081 ctgtatattt ttcataggtt tttctccatc cgagtatcaa tatctgacat attttcttat
     4141 cgttgagaaa aaatattcat ataattgaaa atagttcctc aatttcttgt acgctcgaca
     4201 aaaccaaagg ttgcaaatta tctcaccgtt agaccgggat ccttggttcg agtgcaagcc
     4261 tcgctaacaa acataactca tggggtgggc acatgagcta cgaaatacaa caaatatt
//
LOCUS       DBNHOH010000384         2997 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_663240, whole genome shotgun sequence.
ACCESSION   DBNHOH010000384 DBNHOH010000000
VERSION     DBNHOH010000384.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_663240"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="AC16V7_06840"
     CDS             complement(<1..184)
                     /locus_tag="AC16V7_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011610311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6256870.1"
                     /translation="MIDSQDRIALGKTDIMISPMGIGTWAWGDRIYWGYGRNYSEPEV
                     RTAFETSLNAGINFFDT"
     gene            complement(177..1733)
                     /locus_tag="AC16V7_06845"
     CDS             complement(177..1733)
                     /locus_tag="AC16V7_06845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR6256871.1"
                     /translation="MNSATPRVVVIGAGIGGLTTAAVLAREGMDVTVLEAQVYPGGCA
                     ATYYHQGYRFDAGATLAGGFYPGGPMDLVARATGIKEWQAHLAQAAMMVHLPDDQKIT
                     LWNDERRWSERERLFGDQGIRFWRWQERTADAMWDLAMRLPPWPPQSYRELAGLTQLA
                     SSWLINLPNKTITSQLLSDAVRPVAAHLKNTPDVLRLFTDAQLLISAQATSDRVNALY
                     GASALDLSRRGVVHLEGGMGSIAQSLVRIIQQHGGQVIFRQEATRVALQKGAPKHVET
                     KRGGIFPADIVIANLPPANLRNLLGDNAPSRIKNASTVPQDGWGAFIMYVGIESSVIP
                     EEYPLHHQVVTNTKHAEGNSIFISINPSWDHERAPSGYRAVTISTHTNLTPWWQIYHE
                     DKAQYESRKNEYADKVMSNAEIALPGFRRAAKLILPGTPITFQRFTRRIDGWVGGFPQ
                     THLLRTLSPKIGKYMWMVGDSIFPGQSTAATALGGLRVARHILDQLEYKVYLSSSQKS
                     LALARTSHSD"
     gene            complement(1765..2208)
                     /locus_tag="AC16V7_06850"
     CDS             complement(1765..2208)
                     /locus_tag="AC16V7_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6256872.1"
                     /translation="MRRYRHTFTVKANKQAVADFHSHSSSMASITPPPVIVQMHKAPE
                     NLGENSEMEFTLWLGFLPIRWLASIEDISPTGFTDRQIRGPFKEWSHRHTYIPIDEKH
                     TEIIDEISYSFKEDLYWGLIGWGMATSLPLLFAYRAWKTRRILKS"
     gene            complement(2205..>2997)
                     /locus_tag="AC16V7_06855"
     CDS             complement(2205..>2997)
                     /locus_tag="AC16V7_06855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HIR6256873.1"
                     /translation="DAQTSEGWGSLVEGAEAIVNLAGESIAGDSMKSLILDRWSPARK
                     RTILESRVKAGRAIIQAVQQAKQKPKVVIQASAVGYYGNRGDEELLESSSSADDFLAQ
                     VCKEWEASTAEIESMEVRRAIIRTGGVALSTEGGAFPFMLLQSKLFVGGPIGSGKQWF
                     SWIHIADEARAIRFLIENPEASGAFNLCAPQTITNADFSRTLGRVLKRPSFFPVPGFA
                     MSLTIGEKAGLLLGSQKQIPKHLLDLGFSFKFPDAESALRDLLIK"
BASE COUNT          742 a          773 c          700 g          782 t
ORIGIN      
        1 cggtatcaaa aaaattaata ccagcattca ggcttgtctc aaatgcggtc cttacttctg
       61 gttcagaata gttgcgccca tatccccaat aaattcgatc tccccatgcc catgttccta
      121 tccccatcgg agagatcata atgtctgttt ttcctaatgc tattcgatct tgactatcaa
      181 tcactatgag aagtccttgc cagtgccaat gatttttgag agcttgatag ataaaccttg
      241 tattccaatt ggtccaggat gtgccgtgca actcgaagcc ctcctagggc agtggcagca
      301 gtggattgcc cggggaagat gctatcacca accatccaca tgtatttccc tatcttcggg
      361 ctaagggtac gcagcaggtg ggtttgggga aacccaccta cccaaccatc gatacggcgg
      421 gtaaagcgtt gaaatgtaat tggtgtgcca ggtagaatca atttagcagc ccggcggaag
      481 cccggaagag ctatctcggc gttagacata actttatcgg cgtattcatt tttcctggat
      541 tcgtattgtg ccttatcctc atggtaaatc tgccaccatg gggttaaatt agtatgggtg
      601 cttatggtca ccgcgcgata ccctgaaggc gcccgctcgt gatcccagct tggatttatg
      661 gaaataaata tcgaattacc ttctgcgtgc tttgtattag tgacaacttg atggtgcagc
      721 gggtattctt cggggattac agaactttct ataccaacgt acattataaa tgccccccag
      781 ccatcttggg gaactgttga cgcgtttttt atcctgctcg gagcattatc tcctaataaa
      841 tttcgtaaat ttgcaggggg taaatttgct atgacaatgt cggcagggaa tataccaccc
      901 ctttttgtct ccacatgttt gggcgccccc ttttgtaatg caacccgtgt ggcttcttgg
      961 cgaaatatta cctgtccgcc gtgctgttgg ataattctca ccagggattg ggcgatactg
     1021 cccatgccgc cttcaaggtg aactactcca cgcctagaca aatccaatgc agatgcacca
     1081 tatagtgcat tcactctgtc gctcgtcgcc tgagcagata tcaataattg agcatcagta
     1141 aataggcgta ggacatcagg cgtattttta agatgtgctg caactggtct gactgcgtca
     1201 gataatagtt gcgatgtgat ggttttattt ggtaagttta tcagccagga tgaggcgagc
     1261 tgagtcaggc cggcgagttc acgataagat tggggaggcc aaggtgggag acgcattgcg
     1321 agatcccaca tcgcatccgc agtgcgttct tgccatcgcc aaaatctaat tccctggtct
     1381 ccgaatagcc tttcacgctc gctccaacgg cgctcatcat tccaaagcgt gatcttctga
     1441 tcgtcgggga gatgcaccat catggcggct tgcgcaagat gagcctgcca ttctttgatc
     1501 ccggttgctc ttgcgacaag atccattggg ccgccaggat agaatccccc ggctaatgtt
     1561 gctccagcat caaaacgata gccttgatga taatatgttg ctgcgcagcc tccggggtaa
     1621 acctgagcct ccaagacagt cacgtccata ccttcgcgag cgagaaccgc ggctgtagtc
     1681 aagcccccaa tccccgcacc aattactaca acccgtggcg tagcgctatt cattatcacc
     1741 aacaatctca attggaaaaa ttgattatga tttcaatatg cgccgtgtct tccaggcgcg
     1801 ataagcgaac agtagaggca agctcgttgc cattccccag cctatcaacc cccaatagag
     1861 atcctctttg aatgaataac taatttcatc gatgatctcc gtgtgtttct cgtcaattgg
     1921 gatatatgtg tgacgatggg accactcctt aaaaggccca cggatttgcc ggtcagtaaa
     1981 cccggtgggg gatatatctt caatgcttgc cagccagcga atcggcagga agcccaacca
     2041 aagcgtgaac tccatctcgc tattttcacc taaattttcc ggtgctttgt gcatctgaac
     2101 aataactggc ggaggtgtga tggatgccat actcgatgaa tgagaatgaa aatcagcgac
     2161 tgcctgtttg ttagctttga cagtgaatgt gtgccgatag cgtctcattt gatcaacaaa
     2221 tcccgtaagg ctgattcagc atccggaaac ttaaaggaaa aacccagatc caataagtgc
     2281 ttgggaattt gcttctgact acccagtagc aagccggctt tctcgccgat tgtgagcgac
     2341 atcgcaaatc ctggtacagg gaaaaacgaa ggccgtttta ggacacgccc caaagtgcgt
     2401 gagaaatccg cattggtaat tgtttgtggt gcgcataaat tgaatgctcc gcttgcctcg
     2461 ggattctcaa tgagaaagcg gatagcgcgc gcctcatcag caatatgaat ccaggaaaac
     2521 cactgctttc ctgagccaat aggaccgcct acgaataact tagactgcaa gagcataaag
     2581 ggaaaagctc caccttcggt gctgagtgca acacccccgg tgcggatgat tgctctgcgt
     2641 acttccatgc tctcgatttc cgccgtgctg gcttcccact ccttgcaaac ctgagccaaa
     2701 aaatcatcag cggatgatga gctttcaagt agttcctcat cacctcggtt gccatagtag
     2761 ccaaccgcgg aagcctgaat caccactttg ggcttctgct tggcttgctg aacagcttga
     2821 ataatcgctc taccagcctt tacgcggctc tccaggattg tgcgcttgcg agccggcgac
     2881 caacggtcta gtatcaaaga tttcattgaa tcacctgcaa tcgattcgcc agccaggttg
     2941 acaatggctt cagctccttc aacaagcgat ccccatccct ccgacgtctg agcgtcc
//
LOCUS       DBNHOH010000385         2072 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_149973, whole genome shotgun sequence.
ACCESSION   DBNHOH010000385 DBNHOH010000000
VERSION     DBNHOH010000385.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_149973"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1361)
                     /locus_tag="AC16V7_06860"
     CDS             complement(<1..1361)
                     /locus_tag="AC16V7_06860"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR6256874.1"
                     /translation="MTRVLICANIYTLNPQQPHATAIVIDGERILRLGARQEILTEFA
                     GNGHRVEILDARHQTLIPGLIDAHFHLEQYALAQQMVDCETPTRAECERRVAERAQQM
                     PPGEWVLGHGWNQNDWPEGFGNNTDLDAVSPKNPVYLTAKSLHAAWANSLALEAAGIG
                     AHTSDPPRGRIGRNEHGEANGILFEGAMELVQQALPEPTPQQVAQAIRDVLPKLARMG
                     LTGLHDFDQRLCFSALQILHQREELTLRVVKSIPLEDLPYAAALGLRTGFGDLYLRIG
                     GIKAFADGALGPRTAAMVQPYEGEVDNRGMLLLDSEELFEQGRMAIDNGLCIAVHAIG
                     DRANHEVLNAFTQLRAYEKEQNTTHSFPRFPLRHRIEHVQVIHPDDTRRLAELGIIAS
                     MQPIHATSDMLMADKYWGSRAALAYAWRTQLEHGATLAFGSDAPVESPNPFWGLHAAV
                     TRRR"
     gene            complement(1358..>2072)
                     /locus_tag="AC16V7_06865"
     CDS             complement(1358..>2072)
                     /locus_tag="AC16V7_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR6256875.1"
                     /translation="PQELAELSASIKEHGVLQPLLVSYDNLSGNYTLIAGERRLLAAR
                     QAGLETVPAIVREVNEQQRLELALIENLQRADLTPLEAAEAYRQLVEEYSLSHENIAD
                     RVGKSRAAVTNTLRLLKLPIRVQQALADGRISEGHARALLALPTPEAQEAALRTILKQ
                     DLNVRQTEEIVRKLSGLRPVSTPKANPSPELTALEERLRTKLGTRVKLNRSRKGGTLV
                     IHFYSDEELDALLETLLQE"
BASE COUNT          430 a          547 c          588 g          507 t
ORIGIN      
        1 cgtcggcggg tcacagcggc gtgcaaaccc cagaacgggt tgggcgattc aaccggcgca
       61 tccgacccaa aggctagggt tgccccgtgt tctaattgtg tacgccaagc atacgccaat
      121 gcagccctgg acccccaata tttatctgcc atgagcatat ctgatgtcgc gtggatgggt
      181 tgcatcgagg cgatgatgcc taactctgct aggcgtctag tgtcatctgg atgaatcacc
      241 tggacatgtt caatgcggtg cctcaaggga aatcgaggga aagaatgtgt tgtattctgc
      301 tctttctcat acgctcgcag ttgggtgaat gcattcagca cttcgtggtt ggcgcggtcc
      361 ccaatggcat gtaccgctat acataaacca ttgtcaattg ccatgcgacc ctgttcgaac
      421 agctcttctg agtctaataa gagcatgcca cggttgtcga cctcaccttc atagggttgc
      481 accatggctg cagtgcgcgg gccgagagct ccatcagcga aagctttaat gccgccgatg
      541 cgcaggtaga ggtctccgaa gccagttcgc aacccaagcg cggcagcata gggcagatct
      601 tctagtggga tactcttgac cacgcgtaga gttagctctt cgcgctggtg taagatttgc
      661 agagcggaaa aacaaagccg ctggtcaaaa tcgtgaagcc cggtcaggcc catcctcgcc
      721 aattttggta agacatcccg aatagcctgg gctacctgtt gcggggtggg ctcagggaga
      781 gcttgttgga ccaactccat cgccccttcg aaaaggatgc cgtttgcctc gccatgctca
      841 tttcggccga tccgtcccct gggtgggtcg gaagtgtgcg cgccgatgcc tgcagcttcc
      901 aaggctaggc tgtttgccca tgcagcgtgc agcgatttgg ctgtcaaata tactgggttt
      961 ttgggagata ctgcgtcgag gtcggtgttg ttgccgaagc cctcaggcca atcattttgg
     1021 ttccagccat gccctaacac ccattctccc ggtggcatct gctgagctcg ctcagcgacc
     1081 ctacgctcgc actcagcacg cgttggagtt tcgcagtcca ccatttgctg tgctagagca
     1141 tattgttcta ggtgaaaatg cgcatcgatc aggccgggga tgagcgtctg atggcgtgca
     1201 tccaggatct caacacgatg tccatttccg gcgaattcgg ttaatatctc ctgccgcgca
     1261 cccagcctca aaattcgttc gccatcaatg acgatggcgg ttgcatgggg ctgttgcggg
     1321 ttgagcgtat agatatttgc gcagataaga actctggtca ttcttgcagc agtgtttcga
     1381 gtaaagcgtc tagctcctca tcagaataga agtggatgac cagggttccc cctttacgcg
     1441 agcgattgag ctttacccgt gtgccgagct tcgtccgcag tctttcctct aaggcagtga
     1501 gctctggaga tggatttgct ttgggcgtgg acacaggacg gagtccactg agcttgcgca
     1561 cgatttcttc ggtctgtcgc acattcaaat cctgtttgag tatcgtgcgc agcgccgctt
     1621 cttgagcttc gggtgtgggg agggctaaca aagcgcgggc atgtccttcg ctgatacgac
     1681 cgtcagcaag cgcttgttgt acgcgaatgg gcaatttgag caagcgcaag gtattggtaa
     1741 ccgcggctcg gctcttgcca acccggtcgg caatattttc atgagagaga gaatattcct
     1801 caacaagttg ccggtaggcc tctgcagcct caaggggggt caggtcggca cgctggaggt
     1861 tctcaatcag agccagttcc aggcgttgtt gttcatttac ctcgcgaacg atagctggta
     1921 cggtttctaa gcctgcctgg cgcgcggcca gcaatcggcg ctcaccggca atcagtgtgt
     1981 aattgccgga taaattgtca taactgacga gcaatggttg cagaactcca tgttctttaa
     2041 tagaggcaga tagttcggcc aactcctgtg gg
//
LOCUS       DBNHOH010000386         2633 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1560498, whole genome shotgun sequence.
ACCESSION   DBNHOH010000386 DBNHOH010000000
VERSION     DBNHOH010000386.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1560498"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            392..1432
                     /locus_tag="AC16V7_06870"
     CDS             392..1432
                     /locus_tag="AC16V7_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR6256876.1"
                     /translation="MSIHNKRPTIIQVAKAAGVSTQTVSRVINDRPDVAPETRKRVKQ
                     VIDQLGYQPSALARSLSQRRSYTLGVVTAGLKYTGPSRTLNGITTKAEELGYALLLKE
                     LPSFTTNDIQSLIQFLLARHIDGLLWAVPEIGNNRKWIEMGVDKIPVPIVFITMQSRP
                     GYSIVSVDNHRGGILATQHLLDLGRQHIGHVSGPLEWWEARQRKQGWQEALHAAGRKV
                     TEAHCTEGNWSASSGETAFLQLLQSYPEMDAVFVANDQMALAALQIAHRRNLHIPKDL
                     AVIGFDNIAESAYFYPSLTTINQNQHELGCQAVQELVSLIEAAHSGNQAIEPKSFLLA
                     PELILRESSVVR"
     gene            1465..>2633
                     /gene="araA"
                     /locus_tag="AC16V7_06875"
     CDS             1465..>2633
                     /gene="araA"
                     /locus_tag="AC16V7_06875"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HIR6256877.1"
                     /translation="MEFKKLEVWFITGSQHLYGEATLKKVADHSQEIAREMTQSGKLP
                     IKVVYKPVVTTPDAVTAICREANNAPNCVGLITWMHTFSPAKMWIAGLNALDKPFVHL
                     HTQYNRDLPWADIDMDFMNLNQSAHGDREFGFIGSRMRLRRKIIVGFWQDEQVLAKLG
                     IWMRAASAWHDMQGMKIARFGDNMREVAVTEGDKVEAQIRLGYAVNGYGLGELVNEVK
                     AVSDAEVDRLVAEYDERYLVTEALTPKGSQREALRQAARIEAGLRTFLEKGRFKAFTT
                     TFEDLHGLTQLPGLAVQRLMAEGYGFGAEGDWKTAALVRAMKVMGAGLEGGTSFMEDY
                     TYDLDETTPQVLGAHMLEVCESVAEGKLSLEIHPLSIGGKSDPPRLVFDIRNGPAV"
BASE COUNT          664 a          731 c          686 g          552 t
ORIGIN      
        1 caaaacaatt ggctaaatca tagaattttt gttatatcaa acataccggt ataaatttag
       61 gcctttaatg agccaagcgc cggcctaacc gcccaatatt ttcgtgcgag caaaattcaa
      121 cttccgagaa gactatctcg gaagtttctc tttgatgacc tgcaccaaat acacgccata
      181 tcacaatgaa ctgtttataa gccccttcca cctcccccct acctcaaaca aaccacaacg
      241 ctctagtttg cgcctacttt gagatgctgg cgattccgga cagtccagct ggcggaactc
      301 ggtctcctct tgacaaacat gcccagattt gctattctgt tatcgttccc gataacgata
      361 acagaaagcc ctctaaaggg ttttccctgt gatgagtatt cataataagc gccccaccat
      421 catacaagta gcaaaggctg ctggagtctc cacgcagact gtctccaggg taatcaatga
      481 ccgccccgat gtagctccgg agactcgcaa gcgtgtgaag caggtcattg atcaacttgg
      541 ctatcagccc agcgctctgg ctcgcagcct gtcccagcga cgcagctaca cgctgggcgt
      601 tgtcacggct ggattgaaat acactggccc ctcgcgcact ctgaacggca tcacaaccaa
      661 agccgaagaa ctggggtacg ccttgctcct caaggagctt cccagcttta ccaccaatga
      721 catccaatcg ttgattcaat ttctcctcgc ccgacacatt gatggtctac tatgggcagt
      781 gcccgaaatt ggcaacaacc gcaaatggat agaaatgggc gtcgacaaaa tcccggtgcc
      841 gatcgtattc atcaccatgc agtcgcgccc agggtactca attgtcagcg tggataacca
      901 tcgaggcggc atattggcaa cgcaacactt gcttgatctg ggacgccagc acattggcca
      961 cgtttccgga cccctggaat ggtgggaagc gcgccagcgc aaacagggct ggcaggaggc
     1021 gttgcatgcc gcaggacgaa aagttactga ggcacattgt acggagggaa actggtccgc
     1081 atccagcgga gaaaccgcct tcctgcaatt gctgcaatcc tacccggaaa tggacgcggt
     1141 cttcgtcgcc aacgaccaga tggccttggc tgcattgcaa atagcccacc gtcgaaacct
     1201 gcacatccca aaggatctgg ccgtgattgg cttcgataac atcgccgaat ccgcttactt
     1261 ctatccttct ctcaccacca taaatcaaaa ccagcacgaa ttaggctgcc aggcagtgca
     1321 ggagctggtc agcctgatcg aagctgccca ctcgggcaat caagccatag aacctaaatc
     1381 attccttctt gcacctgagc tgattttgcg ggagagctca gtcgttcgat aaccgcctaa
     1441 tgcttatctt agggaggcat tgtgatggaa ttcaaaaaac tcgaagtatg gttcattacc
     1501 ggaagccagc atttatatgg agaagccacg ctcaagaaag tggcagatca ttcgcaggaa
     1561 atagcgcgag agatgaccca gtcgggcaag ctgccgataa aggttgtcta caagccagtc
     1621 gtgacaaccc ccgatgcggt caccgcaatc tgccgggagg cgaataacgc ccccaattgt
     1681 gtcgggctga tcacctggat gcacaccttc tctccagcga aaatgtggat cgctggattg
     1741 aatgcgctcg acaagccctt cgttcacctc catacacagt acaaccgcga cctgccgtgg
     1801 gctgacatcg acatggattt tatgaatctc aaccagtccg cacacggcga ccgggaattt
     1861 ggatttatcg gcagccgcat gcgattgagg cggaagatca tcgtcggttt ctggcaggac
     1921 gagcaagtcc tggcaaaact tggcatctgg atgcgggctg cctctgcctg gcacgatatg
     1981 caagggatga agatcgcccg ctttggcgat aacatgcgcg aagtggcagt caccgagggc
     2041 gacaaggtag aagctcaaat ccgcctggga tatgctgtca acggatacgg actgggtgaa
     2101 ctggtgaatg aggtgaaggc tgtatcggat gccgaggtcg ataggctggt agctgaatac
     2161 gacgaacgct atctcgtgac cgaggccttg acgccgaagg gtagccaacg tgaagccttg
     2221 cgccaggcag cccgcatcga agcagggtta aggacgttcc tggaaaaagg ccgattcaag
     2281 gccttcacca caaccttcga agatttgcac ggattaacac aactaccggg tttggcggtc
     2341 cagcgcctga tggcagaggg ctacggcttc ggcgcagaag gggactggaa aaccgccgcc
     2401 ttggtacgcg ccatgaaggt catgggagcc ggactggagg gcgggacgtc attcatggag
     2461 gactacacgt acgacctgga tgagactaca ccccaggtgc tcggcgcaca catgctcgag
     2521 gtttgcgaat cggttgccga gggaaaactc agcctagaga ttcatcccct ctccattggc
     2581 gggaaatccg atccgcccag gctggttttt gacatccgca atggtccagc ggt
//
LOCUS       DBNHOH010000387         2104 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_663417, whole genome shotgun sequence.
ACCESSION   DBNHOH010000387 DBNHOH010000000
VERSION     DBNHOH010000387.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_663417"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1211)
                     /locus_tag="AC16V7_06880"
     CDS             complement(108..1211)
                     /locus_tag="AC16V7_06880"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6256878.1"
                     /translation="MPLKGKGFFIWQIRNTEGGNASAIASLADQANLSHVLIKIADGT
                     YSYNVNSSGVDLVPPVAQALRARGIQVWGWHYVYGDSPIDEANKAIQRIQQTNMQGYV
                     IDAEGEYKQPGKDKAAAQFMSRLRSALPNFPIALCSYRYPSYHPQLPWKEFLEKCTYN
                     MPQVYWVSAHNPGDQLRRSVSEFQAMTPFRPIIPVGSAYKSGSWAATASDVTEFLRTA
                     QSLNLSAANFWEWANCRTYLPSVWNAIRDYKWDAEPPPPPPTDITEQYITALNTHNPA
                     QVVGLYTPAAVHVTAARTIQGTVAIQNWYQTLFTQLLPNASFTLVSFSGSGSSRHFTW
                     TATSSAGKVNNGSDTLGLIEGKIAYHFSSFSIT"
     gene            complement(1213..>2104)
                     /locus_tag="AC16V7_06885"
     CDS             complement(1213..>2104)
                     /locus_tag="AC16V7_06885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR6256879.1"
                     /translation="IPPTAAFEPTEIPTETPIPTPEVLTFESLPTDFGPVGSIEEPKG
                     ITNILLLGSDMRSWWGKGSLFRTDTIILASLNPEAGTVSLVSFPRDLYITIPGHGQDR
                     INTAWGRGGFSKLSAAFEYNFGVQVDHYVLIEFSNFKRVVDSLGGLDVQVGERLSDYY
                     RGRLTTIKPGIRTMNADMVLWYVRSRKTSNDFARNRRQQEILQALINKFLSLDAIQRA
                     PELYDLYDESVKTDLNLFDLLPYLPLAAKLGDGKRVKNYFVGSGKVTPYVTAGGAQVL
                     LPQKDKIMRLLRKAMDGQAE"
BASE COUNT          469 a          521 c          608 g          506 t
ORIGIN      
        1 cagatcgaag aaaccgtcta ctcgctcagg cagcgctaag ctagctcctg cgaagcgaac
       61 ccaacggaga agcgggtcaa aataaagctc agttgtgggg atctggtcta ggtgattgag
      121 aaagaactga agtggtaagc gatcttgcct tcgatcagcc ccagagtatc gctgccgttg
      181 ttcactttac ccgcgctcga ggtcgctgtc caggtgaagt ggcgtgagct gccgctgccc
      241 gaaaaactca ccagggtaaa gctggcattg ggcaggagtt gtgtaaacag tgtttggtac
      301 cagttctgaa tggcgacggt accctggatg gtgcgagcgg cggtgacatg cactgctgct
      361 ggggtataca agccgaccac ctgggcaggg ttgtgcgtat taagtgcggt aatatattgt
      421 tctgtaatgt ccgtaggggg tggtgggggc ggctcggcgt cccatttata atctcgaata
      481 gcattccaaa ccgatggcag gtaggtacgg caattggccc attcccagaa gttggctgca
      541 ctcagattga gactttgcgc cgtgcgcaga aattccgtca cgtctgatgc ggtggcagcc
      601 cagctacccg atttatatgc tgatccgacc gggataatgg gacgaaaggg ggtcatggct
      661 tgaaattcgc tcaccgagcg acgtagctgg tcaccagggt tgtgggcgct gacccagtac
      721 acctgcggca tattatatgt gcacttttcc agaaattcct tccagggaag ctggggatga
      781 taggatggat agcggtatga gcacagtgca atggggaagt tgggtaacgc cgagcgcagg
      841 cgactcatga attgtgctgc tgctttatcc ttcccaggtt gtttatactc gccttcggca
      901 tcgatcacgt agccttgcat attggtctgc tggatgcgct ggatggcttt attagcttca
      961 tcaatgggcg agtcgccata cacataatgc catccccaga cctggatgcc gcgcgcccgc
     1021 agtgcctggg ctacaggcgg gaccagatcc acaccgctgg aattgacgtt ataactgtag
     1081 gtgccatcgg cgatcttgat cagcacgtga ctcaagttag cctggtcggc tagggaggca
     1141 atcgccgagg cgttgccgcc ttccgtattg cgaatttgcc agataaagaa ccctttcccc
     1201 ttgagtggca tcttattctg cctgcccgtc catcgccttg cgcaacagtc gcataatttt
     1261 atccttttgg ggcagcaaca cctgcgctcc accggctgtt acgtaaggag tgaccttgcc
     1321 cgagccgaca aaatagttct tcacgcgttt gccatccccc agtttagcgg caaggggcag
     1381 ataaggcagc aaatcgaaca ggttcaggtc cgtttttacg ctttcatcgt acaggtcgta
     1441 tagttcaggg gcacgttgga tagcatccag gcttaggaat ttgttgatca atgcttgaag
     1501 aatctcttgt tggcggcgat tacgagcaaa gtcgttgctg gtctttcggg agcgtacata
     1561 ccagagaacc atatcggcgt tcatcgtccg tataccgggt ttgatggttg tcagacgccc
     1621 gcggtagtag tctgataatc tctctccaac ttggacatcc aaaccgccaa gactatcgac
     1681 aacccgcttg aagtttgaaa actcaatcaa aacgtaatgg tccacctgaa cgccgaagtt
     1741 atattcgaag gcggcggaaa gcttggagaa acctccccgc ccccaagcgg tattgatgcg
     1801 gtcctgtcca tggcccggga tagttatgta caggtcgcgc gggaacgaga ccaggctaac
     1861 cgttcctgct tcgggattca aactagccag tatgattgtg tcggtgcgaa ataggctgcc
     1921 tttgccccac caggagcgca tgtctgaacc aagcagaagg atattggtta tgcctttggg
     1981 ctcttcgata gatccaactg gaccaaaatc ggtgggtagc gattcgaatg tcagaacttc
     2041 cggcgtggga attggggttt cggtggggat ttccgtgggc tcgaaagctg ctgtgggcgg
     2101 tatg
//
LOCUS       DBNHOH010000388         3407 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_984307, whole genome shotgun sequence.
ACCESSION   DBNHOH010000388 DBNHOH010000000
VERSION     DBNHOH010000388.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_984307"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="AC16V7_06890"
     CDS             <1..663
                     /locus_tag="AC16V7_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR6256880.1"
                     /translation="IGSNTLEILGVPSLHGGEYTLGPDYLEVVSFIGAAAITKGSIRI
                     HKAAPEYLDMIAIVFRRLGVSWEVEGEDIVVPAQQSMEVEPDLGGVIPEISVMPWPSF
                     PTDLMSIAIVIATQSKGSVLFHDWMYPSRMFFIDKLVGMGAQIILCDPHRCIVQGPTQ
                     LYGENLESPDIRAGMALLLAALASKGKSVIRNVGQIDRGYERVDEKLYALGAHIKRVH
                     QD"
     gene            complement(725..1075)
                     /gene="trxA"
                     /locus_tag="AC16V7_06895"
     CDS             complement(725..1075)
                     /gene="trxA"
                     /locus_tag="AC16V7_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR6256881.1"
                     /translation="MVQEPIHVTDAAFEKTVLQSTIPVVVDFWAPWCGPCKMIAPTLE
                     KLAKEHAGKLLIAKVNTDEHPEWSMRFGVQGIPTMLFIAGGKVIHRQVGALPEPMLRD
                     IIGQFLEVVQNVES"
     gene            1185..1964
                     /locus_tag="AC16V7_06900"
     CDS             1185..1964
                     /locus_tag="AC16V7_06900"
                     /inference="COORDINATES: protein motif:HMM:NF014654.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR6256882.1"
                     /translation="MYPERVSPKPQPYTVSTPVYQGPLDLLLHLIERAELDITRLALA
                     QVTDQFLAYINNMPELSADEVSTFLVIAAKLIQIKSEALLPRPPVREEGEEDPGEALA
                     RQLLEYRRYKYIADFLSQKEELGLQTYLRLAPPPRIEGVVDLSEIHLDDLVAAAQAIL
                     HKIDPRSSLNSVVSPSPVTIRQKISRIARILRRERRVSFFTLLNGARTRLDIVVTFLA
                     ILELIKQKIVLASQTMQFGNIEIEALKEWDEGVEFELEFDD"
     gene            2006..>3407
                     /locus_tag="AC16V7_06905"
     CDS             2006..>3407
                     /locus_tag="AC16V7_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR6256883.1"
                     /translation="MSNLLANENSPYLLQHANNPVQWYPWGNEALNKAQREEKPIFLS
                     IGYAACHWCHVMAHESFEDPDTASIMNQRFINIKVDREERPDLDNIYMNAVISMIGHG
                     GWPLSVFLTPEGHPFFGGTYFPPTRRYNMPSFREVLLSVYNAWTEDRAHILDVAQQIS
                     SRLAQSTIKSGEYPEVTPETLDNASLALAQTYDWKYGGWGSAPKFPQPMTIEFLLRRA
                     STGDKLALDLATHALRAMAKGGMYDVIGGGFARYSTDNEWRVPHFEKMLYDNALLSQV
                     YLHAYLLTGDKSFRRVCEETLDFIARELSHPSGGFFSSLDADSEGEEGKYYLWTPATI
                     QAAISDKEQLEMVTAAYEITDSGNFEGSTVLQRVIDDASLAVRFAIAEEDVPLRLAEI
                     HKQLLRAREKLVRPATDDKVLVMWNALTLVSFSEAGRYLKRSDYVDMAIRNAKFLLDE
                     LYTQDRLLRSWRNGRSQ"
BASE COUNT          923 a          822 c          781 g          881 t
ORIGIN      
        1 attggctcaa acacactcga aattcttggt gtgccaagct tgcatggggg tgagtacact
       61 ctcggacccg attatcttga agtggtcagt tttattggag ccgctgccat aactaaggga
      121 tcaattcgta ttcacaaagc ggctcctgaa tacttggata tgatcgccat tgttttccgc
      181 aggcttggag tgagctggga ggtggaaggt gaggatatcg tcgttcccgc ccagcagagc
      241 atggaggttg aacccgattt aggaggcgta atacccgaaa tcagcgtgat gccctggcct
      301 tctttcccca ccgacctgat gagcatcgct attgtcattg ccacacaatc caagggtagt
      361 gttttgttcc atgactggat gtaccccagc cgaatgtttt tcatcgacaa attggtcggc
      421 atgggcgccc aaatcatcct atgtgatccc catagatgta ttgtccaagg tcccactcag
      481 ctttacggag aaaatttgga aagcccagat atacgcgcag ggatggctct tttgcttgct
      541 gccttagcga gcaaagggaa atctgtcatt cgcaatgtcg gtcaaattga tcgcggatac
      601 gaacgagtgg atgaaaaact atatgcgttg ggtgcacata ttaaacgagt ccatcaggat
      661 tgagaaataa actacgccca gcactttgcc tgctgggcgt agcaaaacca atccgctaaa
      721 gggcttaaga ttctacgttt tgtactactt ctaaaaactg tccaatgata tcacgcagca
      781 tcggctcagg tagcgctccc acttgccgat gaattacctt cccacccgct ataaacagca
      841 ttgttggaat gccttgcacg ccaaacctca ttgaccactc aggatgctcg tcggtattaa
      901 ctttagctat caatagtttg ccagcatgtt ccttggcgag tttctctagt gttggggcaa
      961 tcattttgca cggcccacac cagggagccc aaaaatccac aacaactgga atggtggatt
     1021 gcaatactgt cttctcaaaa gcagcatcag taacatggat aggttcttga accatcaatt
     1081 tacctcctaa taatcaaatc tttgcattat aaagattaga tgcagatttc ctctaaaagt
     1141 tacaaacaaa gtatatcatt taattgtcag gtataatcca agccatgtat cccgaacggg
     1201 taagtccaaa gccacagcca tataccgttt caacgccagt atatcaaggg ccattggatt
     1261 tattgttgca ccttattgaa cgtgcagagt tagatatcac cagacttgct ctcgctcagg
     1321 ttacggatca gttcctcgca tatatcaaca atatgcctga gctctctgcc gacgaggttt
     1381 ctactttcct ggtcattgca gcaaagttga tccagatcaa gtccgaagct ttgttgccgc
     1441 gcccaccggt gcgtgaagaa ggcgaagagg atccaggcga agctctagcg cgccagttat
     1501 tggaataccg ccgctataaa tacattgcag attttttatc acaaaaagaa gaactgggac
     1561 tgcagacgta cttacgcctc gctcctcccc ctcgcattga aggagttgtc gatttgagcg
     1621 agatacacct ggatgacctg gtagctgccg cccaagccat tttgcacaaa atagacccgc
     1681 gttcatcatt aaattcggtt gtcagtccat ctccggtgac tattcgccaa aaaatcagtc
     1741 gcattgcccg aattctacgc cgagagcgaa gggtatcctt ttttacactg ttaaatggcg
     1801 cgcgcactcg tctggatatc gttgtgacat ttcttgcaat tttagagctc atcaaacaaa
     1861 aaattgtcct tgccagtcaa acaatgcaat ttggcaacat cgaaattgag gcacttaaag
     1921 aatgggacga gggcgtggag ttcgagttag aatttgacga ttagccattt cgtttacccc
     1981 tccctgaacc tgattgataa ttcacatgtc taatttatta gcaaatgaaa attcaccata
     2041 cctgctccaa cacgcaaaca atccagtcca atggtatcca tggggcaacg aagcactgaa
     2101 taaagctcaa agggaagaaa aaccaatatt cctgagtatc ggctatgctg cctgccactg
     2161 gtgccatgtt atggctcacg agtcatttga agatcctgat acagcatcca tcatgaacca
     2221 gcgattcata aatatcaagg tggacagaga agagcgacct gatctagata atatatatat
     2281 gaatgctgtt atatccatga ttggacatgg aggctggcct ttgagtgttt ttctcacccc
     2341 ggagggacat cctttctttg gtgggaccta ttttccacca acccgtcgat ataatatgcc
     2401 ctcatttcgc gaggtcctac tgagcgttta taacgcatgg acagaagacc gggcgcatat
     2461 tttggatgtt gcccagcaaa taagcagtcg gcttgcgcaa tctactatca aaagtggtga
     2521 atacccagag gttacccccg agacgctgga taatgcatcc cttgctttag cacaaaccta
     2581 tgactggaaa tacggtggtt ggggtagcgc gccaaaattc ccgcaaccca tgaccattga
     2641 atttttactt cgccgcgcca gtacgggtga caagctcgct ctcgacctag ctacgcatgc
     2701 tttgagagcc atggctaaag gcggcatgta cgatgtgatt ggaggaggtt ttgcccgcta
     2761 tagtaccgat aacgagtggc gcgtccctca ttttgaaaaa atgctctatg ataacgccct
     2821 actatcccag gtatatctcc atgcctactt gcttactgga gacaagagct ttcgtcgcgt
     2881 ttgcgaagag actttagatt tcatagctcg cgagcttagc cacccatctg gggggttttt
     2941 cagcagccta gacgccgatt cagaaggaga agaaggaaaa tactatcttt ggacgcctgc
     3001 cacaattcag gccgcaatct ccgacaaaga gcaattggag atggtcacag ccgcgtacga
     3061 gataacagat agcggaaatt tcgaaggaag cactgtattg cagcgggtaa tagatgatgc
     3121 atcccttgca gtaaggtttg ccatcgccga agaagatgtt ccactccgct tagcggaaat
     3181 tcataagcaa ttattgcgcg cgcgcgaaaa actagtccgc cctgctacag acgacaaagt
     3241 cctggtcatg tggaacgcgt tgacgttggt ttcgttctca gaagctggta ggtatctaaa
     3301 gcgaagcgat tatgtggaca tggctatacg caatgccaaa ttcttactcg atgagcttta
     3361 cacccaagat cgattactcc gctcttggag aaatggccgc tctcagc
//
LOCUS       DBNHOH010000389         1415 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1624485, whole genome shotgun sequence.
ACCESSION   DBNHOH010000389 DBNHOH010000000
VERSION     DBNHOH010000389.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1624485"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="AC16V7_06910"
     CDS             complement(<1..759)
                     /locus_tag="AC16V7_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR6256884.1"
                     /translation="MSETKHVTLPVTGMTCANCVSTVERNLKKVQGVQQASVNLSSER
                     ASVEYDPSLANLGDLIARVQKAGYGVASGEADLLIRRLSDDNDARRLETSLQKVDGVL
                     EAQVNFASERARLSYIPTIISQSEIRSAVAAAGFEAVETGGEVEDAERKARQAEIAQQ
                     RQYLIVGLIFTIPLFLFAMGRDLNLLPHWAHDPWANWMMLALATPVQFYVGWQYYVGA
                     FKALRNGSANMDVLIAMGSSAAYFYSLPIVFGLIS"
     gene            complement(762..968)
                     /locus_tag="AC16V7_06915"
     CDS             complement(762..968)
                     /locus_tag="AC16V7_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR6256885.1"
                     /translation="MTTVTYSIPNISCGHCVQTIQNEISELDGVISVIANRDMRTATI
                     MFEPPATEEKIQSLLAEIDYPVVA"
     gene            complement(989..1258)
                     /locus_tag="AC16V7_06920"
     CDS             complement(989..1258)
                     /locus_tag="AC16V7_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HIR6256886.1"
                     /translation="MKHDNVSLRLKNVEGHIRGIRRMADQDAYCIDIIRQIQAVQAAL
                     NKISTAILEQHLNSCLTTTVRGDDPDERERVLNEIASVFETATKT"
BASE COUNT          362 a          351 c          348 g          354 t
ORIGIN      
        1 gctgatcaat ccgaacacga tgggtagtga atagaaatac gcagctgaag accccatggc
       61 gatgagcaca tccatattgg cggatccatt acgcagcgcc ttgaatgcac ccacataata
      121 ctgccagcca acataaaact gcaccggagt agccagtgct aacatcatcc aattcgccca
      181 tgggtcgtgc gcccagtgag gaagcaggtt aagatcccga cccatggcaa acaaaaagag
      241 cgggatggtg aatattaatc ccacaatcaa gtactggcgc tgctgggcaa tttccgcttg
      301 gcgtgccttg cgctcagcat cctccacttc cccaccagtc tcaactgcct caaaacctgc
      361 tgcggcaacc gcgctgcgaa tctccgattg gctgattatc gtagggatat agctcaagcg
      421 tgcgcgctcg cttgcaaaat ttacctgggc ttcgagaacg ccatctacct tttgcaggga
      481 ggtttcaagg cgacgagcgt cgttatcatc actcaaacgc cgaataagca agtctgcttc
      541 acccgaggca accccgtagc ctgctttttg gacgcgggca atgaggtctc caaggttcgc
      601 tagcgaagga tcatactcaa cgctcgcgcg ctcagaagac agattgacgg atgcctgttg
      661 gacgccttgt acctttttga gattacgctc aaccgtcgag acacagttgg cacaagtcat
      721 accggtaacc ggtagtgtga cgtgcttggt ttcgctcatg atcaagctac aacagggtaa
      781 tcaatctccg ccaagagtga ttgaattttt tcctctgtgg cagggggctc gaacataatt
      841 gtcgctgtac gcatatcccg atttgcgatc acagagatca caccatccag ttcgctgatc
      901 tcgttctgga tagtctgcac gcaatgaccg caagaaatgt ttggaatgga ataggttaca
      961 gtagtcatgg gtatctcctt agttagattt aggttttcgt tgctgtttca aaaacactgg
     1021 cgatttcatt tagaacgcgc tcccgctcat cgggatcatc gccgcgaacg gtcgtagtaa
     1081 ggcaagaatt taggtgttgt tcaagaattg cagtgctgat tttattgagc gcagcttgga
     1141 cagcttggat ttgccggata atgtcaatac agtatgcgtc ttggtcagcc atccgacgga
     1201 taccgcgaat gtgaccttca acatttttga gacgtagcga gacattatca tgtttcatag
     1261 cactatcctt gttcctaagt atcccacccc ccctgggggg ggtatggtta ttgtatcaca
     1321 tagccatatc ttgtcaagaa aaaagacctg gaaacagaca atttgagata agagggcatt
     1381 atggagccgt tttgaggttc attggcgtaa aaatg
//
LOCUS       DBNHOH010000390         1097 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1304532, whole genome shotgun sequence.
ACCESSION   DBNHOH010000390 DBNHOH010000000
VERSION     DBNHOH010000390.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1304532"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /gene="pyk"
                     /locus_tag="AC16V7_06925"
     CDS             <1..1068
                     /gene="pyk"
                     /locus_tag="AC16V7_06925"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIR6256887.1"
                     /translation="NLELIVTKVHGDRVETQVSLGGTLKPHKGVNLPGAQLDVPSFTE
                     KDEDDLSFGLEHRVDAVAISFVRSAQDILTIRQAIANIAPDRSWIPIIAKLERPEAVV
                     NLEEIVESADGVMVARGDLGVEMSPEAVPIAQKEIIESANNHARIVITATQMLDSMIS
                     KPRPTRAEASDVANAIFDGTDAVMLSGETAAGKYPVQSIETMNAIISQAEPHLSEWGH
                     WKGTCMPELSQDDAYYMTLAARELAHDRNVAAIVVFTKSGRTALLMSKARPHLPILAF
                     TPDPNTYQRLGLYWGVYPNLIPQADTIEEMLQVVETAMVASSSMQAGQQVVVICGFPV
                     SQIRPTNLALLYTIGEISTYT"
BASE COUNT          300 a          282 c          269 g          246 t
ORIGIN      
        1 aatttggaat taatcgtcac aaaagtgcac ggtgaccgcg tcgagacaca ggtatccttg
       61 ggcggtacgc tcaagcctca taaaggggtc aacctgcctg gcgcgcagtt ggatgtcccg
      121 agcttcacag aaaaggatga agatgatctc tccttcggtc tagaacacag agtagatgcc
      181 gtggcgatat cctttgtccg ctcagcccaa gatatcctaa ctatccgcca ggccatcgct
      241 aatattgctc cagaccgcag ctggatacca atcatcgcca aattagaacg accagaagct
      301 gtcgtaaatc tggaagagat cgttgaaagc gcagatgggg tcatggttgc ccgaggtgat
      361 ctgggagttg aaatgtcccc agaggctgtg cccattgctc aaaaagagat cattgaaagc
      421 gctaataacc atgcacggat cgtgatcacc gccacacaga tgctcgattc gatgattagc
      481 aaaccacgcc ccacacgcgc cgaggcatcc gatgtagcaa acgccatatt tgatggaact
      541 gatgctgtaa tgctctcggg agagacagcc gcaggaaaat atcctgtgca atcgattgaa
      601 acaatgaacg ctattatcag ccaggcagaa cctcacctgt ctgaatgggg gcattggaag
      661 ggcacttgta tgcccgagtt aagccaggat gatgcctatt acatgacatt ggcggcacgc
      721 gagcttgcac atgaccgcaa cgtagctgcc atcgtggtat tcaccaagag cgggcgaact
      781 gccttattaa tgtccaaggc gcgtccacat ttacctattc tggcattcac acccgacccg
      841 aacacctacc agcggctggg attgtactgg ggagtgtatc caaacttaat cccgcaagcc
      901 gatacaatcg aagaaatgct ccaagtggtt gaaaccgcca tggtagcttc ttcatccatg
      961 caggctggac aacaggtggt tgtcatttgt ggttttccag ttagccaaat ccgtccgaca
     1021 aatcttgctc ttctctatac aataggagaa atatcaacat atacttgatc catgcatagg
     1081 ctaactgcga tcactgg
//
LOCUS       DBNHOH010000391         1456 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_406758, whole genome shotgun sequence.
ACCESSION   DBNHOH010000391 DBNHOH010000000
VERSION     DBNHOH010000391.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_406758"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..418
                     /gene="kal"
                     /locus_tag="AC16V7_06930"
     CDS             50..418
                     /gene="kal"
                     /locus_tag="AC16V7_06930"
                     /EC_number="4.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019475 - L-lysine fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminobutyryl-CoA ammonia lyase"
                     /protein_id="HIR6256888.1"
                     /translation="MSEHDVHYGGGLVDGARLMALFGDAATELCIRHDGDEGLFRAYN
                     RVEFLRPVHAGDFLEITAHLVGVGRTSRQMRFLVHRTICSAYSTENPSAAEVLSEPEL
                     VAIAEGTCVVPFDKQRRREG"
     gene            422..1249
                     /gene="kce"
                     /locus_tag="AC16V7_06935"
     CDS             422..1249
                     /gene="kce"
                     /locus_tag="AC16V7_06935"
                     /EC_number="2.3.1.247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019475 - L-lysine fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage enzyme"
                     /protein_id="HIR6256889.1"
                     /translation="MQPLIITAAVVGAELSRQDTPFLPLTPDEIAEEAWEAQQAGAAM
                     VHLHARAADGSPTQDIQAYQSIIQAVRSRCPDLIIQVSTGGAVGMSAEERLQPLALQP
                     DMASLTTGTVNFGEEVFYNSLPMIRQFARAMLEKNIRPELEIFDSGMIANALRLFKEG
                     LLLEPLHFNFVLGVPGGAAGTLKNLMHMVESVPPSSTWCASGIGAAQLPLTTMAILLG
                     GHVRVGLEDNIYYRRGELSQGNAPLVARVFRLAGELGRPLATPNEARRILNLEREKA"
     gene            1246..>1456
                     /locus_tag="AC16V7_06940"
     CDS             1246..>1456
                     /locus_tag="AC16V7_06940"
                     /inference="COORDINATES: protein motif:HMM:NF053195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HIR6256890.1"
                     /translation="MNVGNRYGEHRVIYPPGALPQAAQRLDNQAPPLDNEIGITVQAL
                     HITSTSFYRLWNEADENANSFANKIK"
BASE COUNT          312 a          429 c          410 g          305 t
ORIGIN      
        1 gtcctcaata tcggctgcac ctattttgac ggtcaaattg cgccggcgta tgtcggagca
       61 cgatgtccat tacgggggcg ggctggtaga tggcgctcgg ctgatggctc tattcggcga
      121 cgcagccacc gaactatgca tccgacacga tggtgatgaa ggactcttcc gtgcctataa
      181 ccgggtagaa ttcctgcgcc ccgtccacgc gggtgatttc ctggaaatca ccgcccacct
      241 ggtcggtgta gggcgcacgt cccgccagat gcgttttctc gtccaccgca cgatctgctc
      301 agcctacagc actgaaaacc catcggctgc tgaggtgcta agcgagcccg aactggttgc
      361 catcgctgag ggcacctgtg tagtgccatt tgacaaacag cgaaggcggg agggttaaat
      421 ggtgcagccg ctgatcatca ccgctgcagt ggtcggggcg gaattgtccc gccaggatac
      481 gcctttcttg cccctcaccc cagacgagat cgctgaggaa gcctgggaag cccaacaggc
      541 tggggctgca atggtccatc tccatgcgcg cgccgctgat ggctccccca cccaagacat
      601 ccaggcctat caaagcatca tccaggctgt gcggtcccgc tgccccgatc tgatcatcca
      661 agtttctact ggtggagcag tgggcatgag cgccgaagag cgtttgcaac ctctagctct
      721 ccagccagac atggctagtc ttactaccgg cacggtcaac tttggcgaag aggttttcta
      781 caacagcctc cccatgatac gccagtttgc acgtgccatg ctggagaaga acatccgccc
      841 agaattggag atcttcgaca gcggcatgat cgcaaatgcg ctgcgcttgt ttaaagaagg
      901 actgcttctc gaaccgctcc atttcaactt cgttctgggt gtcccgggtg gcgccgccgg
      961 aacgctgaaa aacctgatgc acatggtcga gtcagtgcca ccaagcagca cctggtgcgc
     1021 gagcggcatt ggcgcagccc aactacctct gaccaccatg gccattcttt tgggtggaca
     1081 tgtccgtgtt ggcctggagg acaacatcta ttaccggcga ggtgagctga gccagggcaa
     1141 tgctccgcta gttgcccgag ttttccggtt ggctggcgag cttggtcgcc cgctggcaac
     1201 accaaatgaa gctcgtcgca tcttgaacct tgagcgagaa aaagcatgaa tgtgggtaac
     1261 cgctacggag aacatcgcgt gatttacccg cctggtgcgt tgccgcaggc cgcgcagcga
     1321 ttggataatc aggcgccacc cctcgataac gagatcggta ttaccgtgca ggctttacac
     1381 atcacttcga ccagctttta ccgcttgtgg aacgaagcag atgaaaatgc aaacagtttc
     1441 gccaacaaga tcaagg
//
LOCUS       DBNHOH010000392         1681 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1368595, whole genome shotgun sequence.
ACCESSION   DBNHOH010000392 DBNHOH010000000
VERSION     DBNHOH010000392.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1368595"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="AC16V7_06945"
     CDS             <1..842
                     /locus_tag="AC16V7_06945"
                     /inference="COORDINATES: protein motif:HMM:NF037628.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HIR6256891.1"
                     /translation="RLNAAGRLDTALAALNLLLTSDLGEAGILSQQLDMYNRERQEIT
                     RLIQESAEQMVLADDPQALLLVAAHSDFNAGVVGLAASRLCEKYYRPSIIATRGEEFT
                     RASCRSIPEFHITQALDQCADLLEHHGGHAAAAGFTVVNENVDELIARLRLIANQELG
                     ELDLRPVLQSDMELPLNELKPELLNYLDWLQPTGYGNRAVHFVSRNLRVTRSQAVGRD
                     GSHLKMSVSDGRITYDAIAFRQGHWLEDLPTHVDLLYEFETNEYNGRVSLQLNVRDIK
                     PSS"
     gene            907..>1681
                     /locus_tag="AC16V7_06950"
     CDS             907..>1681
                     /locus_tag="AC16V7_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6256892.1"
                     /translation="MTAFRPANPAFSTSKPSGGHFVPPPLSLIEDTGLSALWLQDLAL
                     KIMYFQGYMTGFKMAEEIALPFNGIVDQILESLKREKFVEVKSSQQMGLGEGSYQYAL
                     TGAGIARAREAMERSQYAGPAPVPLKVYNESILRYSKGRPMVGSRDLRQALSELVLNE
                     RTYQRLGPAINSGTSIFLYGPPGNGKTTIARAVGQMILRESMYIPYALYVDGQVVKLF
                     DSVNHQLLRDEDSTPLGTGSLRSGSRRDPRWLRIRRPFIV"
BASE COUNT          403 a          420 c          427 g          431 t
ORIGIN      
        1 ccaggttgaa cgctgctggt cgcctggata ccgccctggc tgccttaaat cttttattaa
       61 catccgattt gggtgaggct ggcatattat cacagcaact ggatatgtac aatcgcgaac
      121 gccaggaaat tacccggctt atccaggagt ctgctgaaca aatggtactc gccgatgacc
      181 cacaagcatt gttgcttgtg gctgcccact ctgattttaa cgctggcgtg gtggggcttg
      241 cagcctcaag gctttgcgag aagtactacc gaccatcgat tattgccaca cgtggagaag
      301 agtttacacg cgcctcgtgt cggagtatcc cggaatttca cattacccag gcgcttgacc
      361 aatgtgccga tttgttggaa catcatggcg gtcatgcggc ggcggctggt tttacagtgg
      421 tcaacgagaa tgtggacgaa ttaatcgcac gtttgagatt gatcgccaat caagaactag
      481 gtgaactgga cttgcgccct gttctgcaaa gcgacatgga gctgcctctt aacgaactca
      541 agccagaatt gcttaattac ctggattggc ttcaaccaac tggttatggc aaccgagcgg
      601 tccattttgt gtctcgtaat ttgagggtga cacgctcgca ggcggtcggc agagatggct
      661 cccatttgaa aatgtcggtg agcgatggac gcatcaccta tgacgctatt gcgtttagac
      721 agggccactg gttagaagat ttacccaccc acgtggatct actgtatgaa tttgaaacga
      781 acgaatacaa cggtcgggtt tcgcttcagc taaacgtgcg ggatattaaa ccgtccagct
      841 aactccgtgg caaaaagctc gccatgctat aattcaaagg ttacatttga ttgaggagca
      901 taaaatatga ccgcttttcg tcccgcaaat cctgccttca gcacgagcaa accatcaggt
      961 ggtcatttcg tccctccacc gctctcacta attgaagata ccggcctgtc tgctctctgg
     1021 ttgcaagatc ttgctctaaa aatcatgtat ttccagggtt atatgacggg cttcaagatg
     1081 gctgaggaga ttgccttgcc tttcaatggc attgtagacc agatactcga atctctaaaa
     1141 agggagaaat tcgtcgaggt aaagtcatct caacaaatgg ggttgggtga agggtcttat
     1201 caatatgctc tcactggggc tgggattgcc cgcgctcgtg aggccatgga gcgcagccag
     1261 tacgccggcc ctgctccggt tcctttgaaa gtgtataacg aatcgatctt acgttatagc
     1321 aaaggccggc ctatggttgg cagccgcgac cttaggcaag cgctgtctga gctggtgctc
     1381 aacgagcgca cataccagcg cttaggccca gcaatcaatt ctggcacttc gatcttcctc
     1441 tacggccccc ctggcaatgg taaaactacg atcgctcgtg ctgtaggcca aatgattttg
     1501 cgcgagagca tgtatattcc ctatgcacta tatgtcgatg gacaagtggt caagcttttt
     1561 gattcggtca atcatcagct actacgtgat gaagattcca ccccattagg cactggttca
     1621 ttgcgtagcg gctcacgacg agatccacgc tggctacgga ttcgacgccc atttatcgtt
     1681 g
//
LOCUS       DBNHOH010000393         1978 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1496520, whole genome shotgun sequence.
ACCESSION   DBNHOH010000393 DBNHOH010000000
VERSION     DBNHOH010000393.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1496520"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="AC16V7_06955"
     CDS             <1..542
                     /locus_tag="AC16V7_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256893.1"
                     /translation="EVRERYPNDPAVSRLNRNLRGYRWKLMGIRIAGILIGIILLAFL
                     GFLGMGRIRAYLISLTPTPTPTATATPTFTPTPTFTPTLTPTMTPTATPTLTPTPLVV
                     FTQRDVWARNGCYEDFTAVGRIPARANLRLLPSDRRFDNLSRECVLVEYQRDGAAVIG
                     WVLTVDVGIDLPPTPTRSP"
     gene            complement(594..1280)
                     /locus_tag="AC16V7_06960"
     CDS             complement(594..1280)
                     /locus_tag="AC16V7_06960"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR6256894.1"
                     /translation="MSEIINRMIDYPSNGQTTPAFLARPMQDKAPGLVVIQEWWGLVP
                     HIMDVCERFAHYGYVALAPDLYHGQAASEPDEARKLAMELDRDRAVQEIVAALQFLRG
                     LEQVSPKKVGVVGWCMGGGLAVVTAAASQEVDAVVSFYGMPRDLNLAASIRAPLLGLY
                     AEHDHSIPLDLVHAFRKELEAHNVAHDVRIYLGAQHAFFNDTRPNIYDPESARDAWTR
                     TLDWFRTYLT"
     gene            complement(1273..>1978)
                     /locus_tag="AC16V7_06965"
     CDS             complement(1273..>1978)
                     /locus_tag="AC16V7_06965"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6256895.1"
                     /translation="GNPAVDYIAADLSSQAQVRQLADVFQGRYSRLDVLVNNAGAFFL
                     RRQQSVDGIEMTFALNHLNYFLLTHLLLDMLKASAPARIVNVASNAHRRAIMNFEDIE
                     GKRKYSGFQAYGQSKLANLLFSYELARKLEGIAVTANAAHPGFVATNIGKNNGPLAKL
                     VMPIATLRALRPEEGARTPVYVASSPDLEGVSGQYFVKQMPVPSSPESYDLQSARRLW
                     EISAKMTGLLTEASYE"
BASE COUNT          454 a          580 c          535 g          409 t
ORIGIN      
        1 cggaggtaag agaacgttat cccaacgatc cggcggtgag ccgcttgaac cgcaacttac
       61 gaggctatcg gtggaaactc atggggatca ggatagccgg catcttgatc gggatcattc
      121 tcttagcttt cctcggtttc ctgggcatgg gtaggattag ggcttacctg atctccctga
      181 cccccacgcc aacgcctacg gcgacagcca cgccgacttt cacccctaca cccactttta
      241 cacccacgtt gactcccacc atgacgccca ccgcgacgcc cactctgacc ccgactccct
      301 tggtggtctt cacacagcgg gatgtatggg cacggaacgg ctgttacgaa gacttcaccg
      361 cggtgggaag aatacccgcc agagccaact tgcgcttatt gccctcggat cggcgcttcg
      421 ataacctcag ccgtgaatgc gtgctggtgg aatatcaaag ggacggcgca gctgtgatcg
      481 gctgggtgct aactgtggac gttggaatcg acctgccgcc tactccaacc cgttcgcctt
      541 agtctcgtct tttcccccta caaataaacc cagactctag ccaaccacca gcattaggtc
      601 aggtaggtgc ggaaccaatc gagcgtgcgc gtccaggcgt cccgagcgga ttcgggatca
      661 tagatattgg ggcgcgtatc attgaagaag gcatgctgtg cacccaggta gatacgcaca
      721 tcatgcgcga cattatgcgc ctcgagttcc ttcctgaagg cgtgcaccag atcaagcgga
      781 atgctatggt cgtgttcggc atacaaaccg agcaagggag cccggatact ggcagccagg
      841 ttgagatcac gcggcattcc ataaaaagac actaccgcat ccacctcttg gctggcagcc
      901 gcagtgacaa cagctagacc accacccata caccagccca caacaccgac cttcttggga
      961 gagacctgct ccaatcctcg caggaattga agcgcagcaa cgatttcctg cacagcccga
     1021 tcccggtcca gctccatcgc cagcttgcgc gcttcgtccg gctcgctggc tgcctgcccg
     1081 tgatatagat cgggagccaa ggcgacgtat ccatagtgag caaagcgctc gcatacatcc
     1141 ataatatggg gcaccagacc ccaccactcc tggataacaa ccagaccggg cgctttatct
     1201 tgcattggcc tagccaagaa agcgggtgtg gtttgtccat tggacgggta gtcgatcatg
     1261 cggttgataa tctcactcat aggaagcctc cgttaataga ccagtcatct tggcgctgat
     1321 ctcccaaagc cgcctggcgg actgcaaatc ataggattct ggactcgatg gtaccggcat
     1381 ttgttttaca aaatattgac ctgaaacacc ctccagatca ggcgaagaag ccacgtagac
     1441 gggtgtgcga gcgccctcct cgggacggag cgcacgcagc gtggcaatgg gcatcaccaa
     1501 ttttgccaag gggccgttat ttttaccaat attggtcgcc acgaaaccag ggtgggcggc
     1561 gttggcggtc actgcaatgc cttctagctt acgggccagc tcataagaga aaagcaggtt
     1621 ggcgagcttt gattgaccgt aggcctggaa gccggaatat ttgcgttttc cctcgatatc
     1681 ctcgaaattc atgatcgccc tgcgatgggc gttcgatgcg acgttaacaa tgcgagcggg
     1741 cgcgctggcc ttcagcatat ccaaaagcag gtgggtgagc aggaaatagt tgagatggtt
     1801 cagggcaaag gtcatctcga tcccatctac actctgctgg cggcgcagga agaaagcacc
     1861 cgcgttgttt accagcacat ccaggcgtga gtagcgcccc tggaaaacat ctgctagctg
     1921 gcgaacctgt gcctgcgagg ataaatcggc ggcgatatag tccacagccg ggttgcct
//
LOCUS       DBNHOH010000394         3511 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1753175, whole genome shotgun sequence.
ACCESSION   DBNHOH010000394 DBNHOH010000000
VERSION     DBNHOH010000394.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1753175"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="AC16V7_06970"
     CDS             <1..376
                     /locus_tag="AC16V7_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005991016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6256896.1"
                     /translation="RKFGKTKYGLNRTIKVILDLFTVKFLISYAHKPIYLFGGTGTLF
                     MIISTIMLAFLFIRKLLFNTSVLQSTFFQIGIMLMILGVQSILMGLIAELLVRTYHES
                     QRKPTYTVRKVLNNKRAKTIEP"
     gene            376..1518
                     /locus_tag="AC16V7_06975"
     CDS             376..1518
                     /locus_tag="AC16V7_06975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6256897.1"
                     /translation="MRILVLIHEFPPIGGGGGQVAKDICQKLVKLGHEITLITAHTKG
                     QLRKEVIEGIQIIRQPSFRRDAAVADLLAMGAYILFSLYPALRLVRHWRPDLIHVHFA
                     VPAGALAWIVSRLTGIPYLLTVHLGDIPGGVPEKTENWFRWFYPFTPAIWRDAARVVA
                     VSNYTRGLAAKFYPVNVHVIHNGVDLEQLDPGEIRVGSPPTIVFAGRFVSQKNPVQLV
                     KILSGLKDLPWHCVMLGDGPLREEVEWQIRENGLQNRFTIPGWVHPDEVLGWFARSDI
                     LFHPSLSEGLPVVGVQALAMGLAIVASRVGGYVEIVKPDQNGYLYFPDDAAGMRSGLR
                     SLLENPIALREARLQSRRLAAHFDLQLIAQDYLSIIQEVVADLAFV"
     gene            1515..>3511
                     /locus_tag="AC16V7_06980"
     CDS             1515..>3511
                     /locus_tag="AC16V7_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256898.1"
                     /translation="MNSGRFPRDWITTSIALAYTGYLSYAVTRLLETPLTIRLRTLLI
                     AGWLLVVLTCALVVLLVVFRRGDRLYKLLEQGARWVQVLDRRVRGLFYLALMVSIATL
                     SYTYVSRGLMENKALRMAVLLALIVVAVQFWPFQAPRSWGGRFLITLLGGAFSLLSGD
                     YLQYAVNYPFSLHWSEGNRLYDYSLIFGKGLYQYDGNLSLPYYAPGRYGLWGIWFLIP
                     GLPISFHRFWNATLWIVPPILLGWLLARSLSRQPGVRLGVALWLGLFLSQGPIYAPIL
                     LVASLLVAFDRSKTRERYIAIGVASLYAGLSRWTWFGAAGAWAALSQICAAEVKPRQP
                     YLKRYIQIVWVIIVGSLPGILANWTQFFSPKDIAFPLSQPKLWYRLFPNSTYPLGIIP
                     GLALACGPLLVILIWLVSSRRFKLGKLQLVAIGGVMVVTLVAGLLASVKIGGGSNLHN
                     LDMFFITLALIVMLYLDQTTTQEDYESFDNPRRSDDEMQQDESSPSLMRGWPYWAKAV
                     MVVASLMLAWPYFSNVRTLKLPPQEDIQRSLDNLRLKIERYKGKGDILFLDQRQLLTF
                     EVIKDIRLLPVYEKKYLMDQAMAGNAVYFQDFHNDRANKRFALIVSEPLFMSYDNEYD
                     PFGEENNAWVKWVSEPVLCFYAPEKTYKEMRVQLLLPRTGT"
BASE COUNT          796 a          865 c          907 g          943 t
ORIGIN      
        1 ccgtaaattt ggcaaaacca aatatggact aaaccgcacg attaaagtca tcttggacct
       61 tttcacggtc aagttcctga tcagttatgc gcacaagccc atttatcttt ttggaggaac
      121 tgggacgctc ttcatgatca tcagcacgat catgctggct ttcttgttca tccgcaagtt
      181 gcttttcaat acaagcgttt tgcaatcgac cttttttcaa atcggcatca tgttaatgat
      241 tcttggcgtc caatccatcc tgatgggctt gatcgccgag ctgctcgtgc gcacctacca
      301 tgaatctcag cgcaaaccaa cctacactgt gcgcaaggtt ttgaacaaca aacgtgctaa
      361 aaccattgag ccctaatgcg catcctcgtt ttaattcatg aattcccccc gattggcggc
      421 ggcggtggac aggtagccaa ggatatctgc cagaagttgg ttaagctagg gcatgaaatc
      481 accctgatca cagctcacac gaaggggcaa ttgcgcaagg aggtcatcga aggcatccag
      541 atcatccgcc agccttcctt tcggcgcgat gccgccgttg ctgatctgct tgctatgggg
      601 gcatacatcc tttttagctt gtatcctgct ttgcgcctgg ttaggcactg gaggcctgac
      661 ctgatccacg ttcacttcgc tgtcccagct ggcgcattag cctggattgt atcgcgccta
      721 actggcatcc cgtatttatt gaccgtgcac ctgggcgata tacccggtgg tgtgccagag
      781 aagaccgaga actggttccg ctggttttat ccttttactc cggcgatatg gagagatgcg
      841 gcccgagtgg tggcagtgag caattatacg cgcggattag cggcgaaatt ctatccggta
      901 aatgtgcacg tcatccataa tggtgtggat ctagaacagt tagacccggg cgaaatccgg
      961 gttggatcac cacccaccat cgtttttgcc gggcggttcg tctctcaaaa aaacccggta
     1021 caattagtga aaattttgtc agggttgaag gatcttccct ggcattgtgt aatgcttgga
     1081 gatgggcccc tacgcgaaga agtggaatgg cagattcggg aaaacgggtt gcagaaccgc
     1141 ttcaccatac cgggttgggt tcatccggat gaagtactcg ggtggttcgc ccgcagcgac
     1201 atcctcttcc atccctcact ttccgaggga ttacccgtgg tgggtgtcca ggcactggcg
     1261 atgggcttgg caattgtcgc cagccgggtc ggaggttatg tcgagattgt taaaccagat
     1321 cagaacggct acctttactt tcctgatgac gctgccggaa tgagaagtgg tctgcggagt
     1381 ctgctggaaa acccaatcgc gttacgggaa gcccgtttac agagccgtcg actagccgct
     1441 cactttgatc tacagcttat agcacaggat tacctgagca ttattcaaga agtggtggca
     1501 gatttggctt tcgtatgaac agcgggcgct ttccgaggga ttggatcact actagcatcg
     1561 ctttagccta taccggctat ttaagctatg cagtgacacg cctgctagag acacccctaa
     1621 ccatccgatt gcgtactctc ttgatcgccg gatggttgct ggtcgtcctg acctgtgcgc
     1681 tggtggtatt gctggttgtg ttcaggcgag gcgatcggct ctacaaatta ctggaacagg
     1741 gggcgcgttg ggtgcaagtt ctggataggc gagtccgtgg gttattctat ttggcactga
     1801 tggtgagcat cgccacgttg agctacacct acgtcagccg tgggttaatg gaaaacaaag
     1861 ctttacgaat ggcggtcttg ctagcattga tcgtagtagc agttcagttc tggcctttcc
     1921 aagcaccaag aagttggggc ggacgcttcc tgataactct tcttggaggg gcgttctctc
     1981 ttttgagcgg cgattatcta caatatgcgg tgaattaccc gttttctctt cactggtcgg
     2041 aaggcaaccg tttatacgat tactcgctta tctttggtaa aggcctttac caatacgatg
     2101 gcaacctatc gctaccctat tatgcaccag gtagatatgg gctgtggggt atctggttct
     2161 taattcctgg cttgccgatc tccttccacc gcttctggaa tgctaccttg tggattgtac
     2221 cgcctattct cctgggatgg ttgctggcgc gaagcctctc caggcaaccg ggcgtacgcc
     2281 ttggcgtggc gttgtggctt ggtttgttct tgagccaggg tccaatctat gcccctatct
     2341 tgctggtagc ttccttgtta gtcgcatttg atcgatctaa aactcgagag cgttatattg
     2401 caatcggtgt ggcaagcctc tatgcaggtt tgagtcgctg gacctggttc ggggcagcag
     2461 gcgcctgggc ggcgctaagc cagatctgcg cggctgaagt aaaacccagg caaccatacc
     2521 ttaagcgcta catacagata gtctgggtca tcattgtcgg tagtctgccg ggaatattag
     2581 cgaactggac acagtttttc tcacctaaag atattgcttt cccactctct caacccaaac
     2641 tatggtaccg cttgttccca aacagcacat atccattggg aatcatacct ggattggctt
     2701 tagcttgtgg gccgttgcta gtgatattaa tctggctagt gtcttcgcgc cgtttcaagt
     2761 tgggtaagct gcaactggta gccattggcg gtgtaatggt cgttacccta gtcgcaggtt
     2821 tgctcgccag cgtaaagatc ggcggtggca gcaatctgca caacctggat atgtttttca
     2881 tcaccctggc actcattgtc atgttgtacc tagatcagac aaccacacag gaagattatg
     2941 aaagctttga caatccaagg agatcggatg atgaaatgca gcaggatgag agctccccct
     3001 cgctgatgcg aggttggcct tattgggcga aggcggttat ggttgtagcc tctcttatgc
     3061 ttgcctggcc atacttttca aacgtccgaa cgctgaaact gccaccgcag gaagacatcc
     3121 aaagatcgct agataatctg cgcctaaaaa ttgagcgtta taagggcaag ggtgatatct
     3181 tattcctgga tcaacgccag ctactaacct tcgaggttat caaagacatt cgcctattgc
     3241 cagtgtatga aaagaagtat ttaatggatc aggccatggc tggtaatgct gtctatttcc
     3301 aggatttcca caacgatcga gccaacaaac gatttgcctt aatcgtatcg gagccgttat
     3361 tcatgagcta cgataatgaa tatgacccct tcggcgaaga aaacaatgcc tgggtgaaat
     3421 gggtctcgga accagtgcta tgtttttacg ctcctgagaa gacctacaaa gaaatgcgtg
     3481 tccaattact cttgccacgt acaggcacat t
//
LOCUS       DBNHOH010000395         4207 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_406938, whole genome shotgun sequence.
ACCESSION   DBNHOH010000395 DBNHOH010000000
VERSION     DBNHOH010000395.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_406938"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="AC16V7_06985"
     CDS             complement(<1..265)
                     /locus_tag="AC16V7_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256899.1"
                     /translation="MNGTISPANQPTSEPRIKPQAWRTLSFFAAVTMELSWVALWIQL
                     LLATRIMISYWRAFLTCGAILLFSYYLDELNLRRNIHAAKRRVI"
     gene            complement(316..1512)
                     /locus_tag="AC16V7_06990"
     CDS             complement(316..1512)
                     /locus_tag="AC16V7_06990"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR6256900.1"
                     /translation="MPIQRWLPLFIIIFYLGIVLRSPFLTAFSSTLLVIFGIAYWWHK
                     HALDGVRYTRRFRYTRGFPGEHFPVRLEIENNKLLPLSWLRVTDPWPKAVGPEDERAL
                     TPSHIADQGLLIHAFSLRWFERARRMYELYLRSRGVYKVGPVQVQSGDFFGIYKQSDR
                     LGSVDQITVFPTLIPLEELKLPPENPFGDQRSRRRFLEDPNFPMGVREYRPEDGFRRV
                     HWPATARTGQLQVKVFQPTSALTLVVCLNVLTYQHSWEGINPALFERLLSIAATLLYQ
                     GIEHGYRVGLISNGCLSNSDQPFHIPPGRSPKQLGRLLEALAGASPVMAKPFENFLMR
                     EIPHVPYGSTLVVVTALTSPALVETLSRLKKHERQITLFSLAEEPPPDILGVRCLHMP
                     FKEEEV"
     gene            complement(1514..2449)
                     /locus_tag="AC16V7_06995"
     CDS             complement(1514..2449)
                     /locus_tag="AC16V7_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6256901.1"
                     /translation="MIIDIAQRLRQNIQKVIIGKDEVINLTLTVVLCEGHMLLEDVPG
                     MGKTTLARALAASLGCTFRRIQFTPDLLPSDITGISCFNQKTQEFEFRSGPVISQVVL
                     ADEINRATPRTQSALLEAMQERQVTVDGVTRPLPRPFLVMATQNPIELEGTFPLPEAQ
                     IDRFLMRIALGYPEESEENAILERFRLADPLVDLEPVTTPQEILEYINQRRQVRIADP
                     VREYIVRVARATRQNTEIELGASPRATLSLYQAAQAWAAIHGREYVLPDDVKNLAPYI
                     LSHRLMISPQAQLRGRKIEELISDIVEAVPVPVEG"
     gene            2634..>4207
                     /locus_tag="AC16V7_07000"
     CDS             2634..>4207
                     /locus_tag="AC16V7_07000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HIR6256902.1"
                     /translation="MSSIVVLDIETTGLDSQKDAVIEIGAVRFNGRRVEDEWSTLINP
                     GRRIPPYITQLTGITDQMVLQAPAIKNVLQELVDFTGNSPVLGHNVGFDLSFLRRYNI
                     LLTNEPIDTYELASVLLPGARRYNLGALAQELSVPLRATHRALDDAHTTRGVFLRLID
                     LIMELPLNLLAEIVRLSEPLEWGGSWAFRQALRSRTKEIVNAQQVRHAYQGPIYTEAL
                     PRPSAPLHPLDKPVSLDIDDTAALLEHGGAFSHHFPQYEYRPQQVAMLRAVAEAISYS
                     RHLMVEAGTGTGKSMAYLIPATLWAIQNNNRVIISTNTINLQDQLINKDIPDLRNAMG
                     LDVQAVVLKGRSNYFCPRRLEILRRRSPETSEELRVLAKVLVWLQNTRTGDRAEINLN
                     GPIERAIWSRISAEDEGCTSENCIKRTGGICPFHRARQAAQSANILIVNHALLLSDVA
                     TGNRVLPEYQTLIVDEAHHLEDATTNALSYRVTQWDIERLLRELGGPRGGALGWLLAA
                     AQEVLGPDEFVAFEQLA"
BASE COUNT         1026 a         1134 c         1083 g          964 t
ORIGIN      
        1 taataacccg acgcttagca gcatgaatat ttctgcggag attcaactca tccaaatagt
       61 atgaaaacag gagaatagcc ccacaggtta agaatgccct ccagtagctg atcattatcc
      121 tggtcgcaag aagcaattga atccacaatg ccacccagct cagctccatg gtaacagcgg
      181 caaaaaacga caatgttcgc caggcttgag gctttattct cggctcgctc gttggctggt
      241 tggctggaga gattgtcccg ttcacaagct cacatccatc gtgatcacat cttggttaat
      301 cgtgcaagga cacatctaca cctcttcctc cttgaaaggc atgtgcaggc agcgcacgcc
      361 caagatatcg ggcggtggtt cttcagctaa cgagaacaat gtgatttgcc gttcgtgttt
      421 ctttaatcta gagagagtct ctaccagggc aggggatgtc aaagcagtca ccacaaccag
      481 tgtcgagccg taaggaacat ggggtatttc tcgcatcaag aagttctcga aaggtttcgc
      541 catcactggc gatgcacccg ccagcgcctc taacaagcgt cccaattgtt tgggggagcg
      601 cccagggggg atatgaaacg gttggtctga attcgaaagg catccattgg atatcagacc
      661 aacccgataa ccatgctcaa tgccttgata taacaaagtc gccgcgatgc ttagcaagcg
      721 ctcaaataat gcaggattta tcccttccca ggaatgctga tacgtcaaga cgttcaggca
      781 tacgaccagg gtcagggctg acgttggctg gaaaactttc acttgcaatt gtcctgtgcg
      841 ggctgtcgct ggccaatgca cgcggcggaa gccatcctcc gggcgatatt cacgtacccc
      901 catcgggaag ttgggatctt cgagaaatcg ccttcgagag cgttgatcgc caaaggggtt
      961 ttcagggggc agcttcaact cttcgagggg aataagcgtt ggaaagaccg ttatctgatc
     1021 taccgaaccc aaacgatcag attgtttata gattccaaaa aaatcaccag actgcacctg
     1081 tactggtccc actttataaa ctccacggct tctcagatat agctcgtaca tgcgccgagc
     1141 ccgctcaaac caacgtaaac tgaatgcgtg gatcagtaat ccctggtctg cgatgtggct
     1201 tggcgtgaga gcgcgctcgt cctccggacc cacagctttt ggccagggat ccgtgacgcg
     1261 caaccaggat aatggaagca atttattatt ttcgatctca agccgtacgg gaaagtgctc
     1321 acctgggaag ccgcgtgtat aacgaaagcg ccgcgtatag cgcaccccat ccagggcgtg
     1381 tttatgccac cagtaggcta ttccaaaaat gacaagcagt gtggaggaga aggctgtcag
     1441 aaatggcgag cgcagaacta tcccaagata aaagatgata atgaataagg ggagccagcg
     1501 ctggatgggc acgtcagccc tccacgggca cgggcacagc ctcgacgatg tccgagatta
     1561 actcctcgat cttcctgcca cgcaactgcg cctggggaga gatcatcagg cgatgactga
     1621 gtatgtatgg cgccaaattt ttcacatcgt caggcaaaac gtattcgcgt ccgtgaatgg
     1681 cagcccaagc ctgggctgct tggtagagtg aaagggttgc tcgggggctg gcacccagct
     1741 cgatctctgt attttggcgg gttgcgcgag ccactcgcac aatatactct cgaacagggt
     1801 cagcgatacg cacttgccgg cgctggttaa tgtactcgag tatctcctgc ggtgtagtca
     1861 ctggctccag atccaccaaa ggatccgcta ggcgaaatcg ttccaggatt gcattctctt
     1921 ctgattcttc agggtagccc aaagcaatcc gcatcaagaa gcggtcaatt tgcgcttccg
     1981 gcagtgggaa agtcccttcc agttcgatcg ggttctgcgt ggccatcacc aaaaaaggtc
     2041 gtggcagcgg gcgcgttacc ccatccacgg tgacctgccg ttcttgcatg gcctccaata
     2101 atgcagactg cgtgcgcggc gtggcgcggt tgatctcatc tgccagcacc acctggctta
     2161 tcaccggacc cgaacgaaac tcgaattctt gcgttttctg attaaaacag ctgatcccgg
     2221 taatatcaga aggcaacagg tctggcgtaa attggatccg gcgaaatgta cagcccaacg
     2281 aagctgctaa ggctcgagcc agggtggttt tacccatgcc gggaacatcc tcaagcagca
     2341 tgtggccttc gcagagcacc actgtcaggg tgaggttaat cacctcatcc ttaccgatga
     2401 ttaccttttg gatattttgt cgtaagcgtt gagcgatatc gataatcatt ttctacaatg
     2461 aagtttacca gaaataccat caccagccat tacaacggct gcctcggata ttcccaaaga
     2521 actaatatca ttacccgcga cacgggtatc aagttcatca aatgattagc gtctttagat
     2581 atccgtggtc agagtggtat acggcatttc tacagcagag tataatcctt tgcatgagct
     2641 ctatcgttgt cctggatatc gaaactaccg gactggattc gcaaaaagac gcggtcatcg
     2701 agatcggtgc tgtgcgcttc aacggtcgtc gtgtcgagga cgaatggagc acgctgatca
     2761 atccaggtcg ccgcatcccc ccctatatca cccagctgac cggcattaca gatcaaatgg
     2821 ttttgcaggc tcctgccatt aagaatgtgc tgcaagagct tgttgatttc actggaaata
     2881 gccctgtcct cggtcacaat gttggctttg atttatcctt cctacgccgg tataacattc
     2941 tgctgacgaa tgagcccatc gacacctatg aactagcctc tgtattactt cccggtgcgc
     3001 gccgttacaa cctgggcgcc ctggcacagg agctgagcgt tccgctgcgg gccacccatc
     3061 gcgccctgga cgatgcccat accacgcgcg gggtcttcct gcgcctcatt gatctgatca
     3121 tggagttacc actgaattta ttggcggaaa tcgtgcgctt gagcgagccg ctcgagtggg
     3181 gtggctcgtg ggcatttcgg caagctctgc gctcacgcac gaaagaaatc gtcaatgccc
     3241 aacaggttcg tcacgcctac cagggtccaa tatacactga agccttaccc cgtccttcgg
     3301 ctcctctaca tcccttggat aaacctgttt ccctggatat cgatgatacg gctgcgttac
     3361 tagagcatgg aggggcattc tcccatcatt tcccgcagta tgaataccga ccccagcaag
     3421 tagccatgtt gcgagctgtc gcagaggcca tttcctatag caggcatctg atggttgaag
     3481 ctggcaccgg gacgggaaaa agcatggcat atctcatacc tgccacgctg tgggctatcc
     3541 agaacaacaa ccgcgtcatc atttctacca ataccattaa cctgcaggat cagctcatca
     3601 acaaagacat tccagacttg cgcaacgcga tggggttgga tgtgcaagcc gtggttttga
     3661 agggccgctc gaactacttt tgcccgcgca ggttagagat tctacggcgt cgtagcccag
     3721 agacaagcga agaattgcgt gttttggcaa aagtgctggt ctggctgcaa aatactcgca
     3781 caggtgaccg agctgaaata aacttgaacg gtcccatcga gcgcgccata tggagtcgaa
     3841 tctccgccga ggatgaaggc tgcacctccg agaattgcat caagcgcacg ggcggcattt
     3901 gcccatttca ccgggcgcgc caggcggctc aatcagccaa tatcttaatc gtcaaccacg
     3961 ctctactgct ttcggatgta gctactggta accgtgtctt acccgaatat caaaccctaa
     4021 tcgtagacga agcccaccac ctggaggatg caacgaccaa tgccttgagc tatcgcgtca
     4081 cccaatggga tatagagcgc ctgttgcgag aattgggagg accgcgaggt ggtgcgctcg
     4141 gctggctgct ggcggctgcc caggaggtac ttggaccaga tgagtttgtc gccttcgaac
     4201 aattagc
//
LOCUS       DBNHOH010000396         5393 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_150319, whole genome shotgun sequence.
ACCESSION   DBNHOH010000396 DBNHOH010000000
VERSION     DBNHOH010000396.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5393)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_150319"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..818)
                     /locus_tag="AC16V7_07005"
     CDS             complement(96..818)
                     /locus_tag="AC16V7_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256903.1"
                     /translation="MHYIPILSTLVSFAFAVAVLNRYRVRKGAHLLFWGIGLILYGLG
                     TLSEVILGFAFNALVLKIWYLSGAMLTAAWLGQGTIFLLVRRSSVATGLALFLVVLSL
                     ASLALILAAPVSGAAAAYDISQPISGQYKEILSRNGAIIALTIFLNLYGTVGLVGGAL
                     YSAYLFWRKRVLAYRMWGNFLIAAGALMPAMAGSFIKAGLVDWLYLSELIGVVLMYLG
                     FLRATSRSQVEADVLASALSNL"
     gene            complement(910..1059)
                     /locus_tag="AC16V7_07010"
     CDS             complement(910..1059)
                     /locus_tag="AC16V7_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256904.1"
                     /translation="MTDKRPSKRFKPTTWMERLVPLLLGLLALALLAVFVVVVLAVLG
                     LTPGT"
     gene            complement(1059..1685)
                     /locus_tag="AC16V7_07015"
     CDS             complement(1059..1685)
                     /locus_tag="AC16V7_07015"
                     /inference="COORDINATES: protein motif:HMM:NF024139.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR6256905.1"
                     /translation="MQVEWLSLSDVAKLLGIHPSTVRLWSDKGVLPVHRTQGGHRRYL
                     RSELDLWIQSQRVGGTNEVHLVVQNALKNTRFQISEGRLEAEAWYAKLDNEAREQYSK
                     SGRTLLEGLFGYLASDGAMAVAEARSLGYEYASRGRRYGLNVTEAVHAFLFFRNVLMD
                     SMLTVYEAASVQSASAWGGMFRKINEFTDQILITLLETYEAYERGNNR"
     gene            complement(1725..3026)
                     /locus_tag="AC16V7_07020"
     CDS             complement(1725..3026)
                     /locus_tag="AC16V7_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCD family MFS transporter"
                     /protein_id="HIR6256906.1"
                     /translation="MLLKRLQLGLIHVAVAMTLVPINSTLNRVMIKELALSATLVALL
                     ASLPYLFSPIQMAIGSFSDRHPILGLRRSPYIALGLILCVLGVVLSPQAAFLLSSNFW
                     AGFGLGLVTFGAWGMGFNFATVSYLSLASELSGEKGRSKTIAVMWFMMISSIIVTAIA
                     LSQMVDPYTPQALSRAFWIIGLAALVLGSLGLLGLERRSVGGQTASEGYSWGIMLAAI
                     LENRQARLFFWYLTVLLAAILGQDILLEPFGGEAFGMSVQATTRITSLWGGCVLLALL
                     LAGFLEGRLGKRVVARWGALSALLGFALIVVSGITLSQGVFYLGVVLLGVGTGLSTVS
                     NLSLMLDMTTAENVGLFMGAWGMSNAVSRLLGTVLGGVVRDGVNQVAGNPLVGYISVF
                     AIEAGLLAVSLLLLRRIDVSAFQKKAASISVVERAAMAGEV"
     gene            complement(3206..4696)
                     /locus_tag="AC16V7_07025"
     CDS             complement(3206..4696)
                     /locus_tag="AC16V7_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009786578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR6256907.1"
                     /translation="MSIRQAYPKYPLLYQINARVYARSPRWGGSGKLDEIPESELDEL
                     ARMGFDWVYLLGVWQTGAVARKISQSIPELLREYHEALPDLRPQDICGSCFAIKSYKV
                     RRDLGGNAALKRFRKRLSQRGLRLMLDFVPNHTAPDHPWVETHPDYYVHGTPELLAQQ
                     PHNYTRLKKTPGAPVWAYGRDPYFPGWSDTLQLNYGNPELRQAVVGELAKVAALCDGV
                     RCDMAMLLLPEVFQRTWGIPAAPFWPQAIQEVRSIFPDFVFMAEVYWDLEWTLQQQGF
                     DYTYDKQLYDRLRERQARQVRQHFDAALDYQNKSVRFLENHDEPRAAKVFSSDEHRAA
                     AVLTYLSPGLRFFHQGQIQGWQTKIPVQLCRAPDQPTDRLVNAIYLRLLELLHDDILR
                     NGEWRLLECTPAWENNASWDSLIAFSWQGSGDRQWLAVVNYAPHPSQGYVRLPFPELG
                     GATLRLKDLLSSAGYDRQGDDLLAHGLYLDLPPWEVNVFEVVRLTE"
     gene            complement(4880..>5393)
                     /locus_tag="AC16V7_07030"
     CDS             complement(4880..>5393)
                     /locus_tag="AC16V7_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256908.1"
                     /translation="AIFLSLSLAACAAIRDSNAPVQTGLATGVGGQPGSCPVTQPPDP
                     PYAPPPSYSSPEEGAFWYGSDALWTMLPLDGTWRGLPFSDNGYTQKTIWWQQDYDWQA
                     DPQPDLTVSGERLDASAPPLPASGATNGYNPDLGSFMLVGVDIPTEGCWEITGQAGDQ
                     SLSFIVWVEP"
BASE COUNT         1306 a         1649 c         1433 g         1005 t
ORIGIN      
        1 ttgcgccgga tacgggcaaa gaatgaatta tcgacaaggg tagaggtagt tgtgctcacg
       61 agataattct accactgggg gaatctagat ttacgctata aattggatag ggccgaagcc
      121 agcacatctg cctccacctg cgagcgcgac gtagctctca agaaaccgag atacatgagc
      181 accacgccga ttagctcgct cagataaagc cagtcgacca gcccagcctt gataaatgag
      241 cctgccatgg ctggcataag cgccccagcg gcaatcaaaa aattgcccca catacggtat
      301 gccagcaccc gcttacgcca gaacaagtag gccgagtaca gcgccccgcc aaccagtccc
      361 actgtgccgt acaggttcag gaaaatcgtc aaggcaatga tagccccatt gcggctgagg
      421 atctccttgt actgcccgga tatcggctgg ctgatatcgt aggcagccgc cgctccgctc
      481 accggcgctg ccaggatcag agcaagcgac gcaagcgata gcacaaccag gaagagcgcc
      541 agtcccgtcg ccacactgga gcgtcttacc agcaggaaga tcgtcccctg tcccagccag
      601 gctgcagtga gcatcgcccc gctgagatac cagattttca acaccagggc gttaaacgca
      661 aagcccagaa tcacctctga aagcgtcccc agcccatata agatcaaccc aatgccccaa
      721 aacaacaggt gagcaccttt tctaacccgg tagcgattta agaccgccac cgcaaaggca
      781 aagctgacca gcgtggataa aatcgggatg taatgcataa ccttctcctt tttatgtttc
      841 cccatccccc ttaccccctc ccccgcttcc gggggagggg gtaggggtgg gggggaaaaa
      901 taattccact caagttcctg gcgtcaaacc caacaccgct aggaccacca cgacgaagac
      961 ggccagcaac gccaaggcga gcaatcccaa gagcagcggt acaaggcgct ccatccaggt
     1021 ggtaggttta aagcgtttgc tcggccgttt gtcggtcatc agcggttatt tccacgctca
     1081 tacgcttcgt aggtctctaa cagggtgatc aggatctggt cggtgaattc gttaatcttg
     1141 cggaacatac cgccccaagc cgaagctgac tgcaccgagg cagcctcata taccgtcaac
     1201 atcgaatcca tcaatacatt gcggaagaac aagaaggcat gcaccgcctc ggtaacgttt
     1261 aagccgtaac gccgtcctcg cgaggcgtat tcatagccca gagaacgcgc ctcggcaaca
     1321 gccatagccc cgtcggaggc taaatagcca aataagcctt ccaggagagt ccggccactc
     1381 ttgctatact gctcgcgggc ctcgttatcc agtttggcat accacgcctc agcctccagg
     1441 cgcccttcac tgatctggaa gcgggtgttc ttcaaagcgt tctgcaccac caggtgcact
     1501 tcgtttgtcc caccaacgcg ctgcgattga atccacaagt ctagctcact gcgcaggtag
     1561 cggcggtggc cgccctgtgt gcgatgaacc ggcagcacgc ccttatccga ccacaagcgc
     1621 acagtgctgg gatggatacc cagtaacttg gccacgtcgc tgaggctcag ccactcaact
     1681 tgcatctagc cctcacctcg atatggattt gaaaattcat ctgatcacac ctcgccagcc
     1741 atagccgccc gctctacaac agagatcgaa gcggcctttt tctgaaaagc gctcacgtcg
     1801 atgcggcgca gcaacaacag ggagaccgcc agcaagcctg cctcgatggc gaacacgctt
     1861 atgtagccaa ctagcgggtt gccagccacc tgattcaccc catcgcgcac cacaccgccc
     1921 aggacggtgc caagcaagcg cgagacagca ttcgacatgc cccacgcccc catgaacaag
     1981 cccacattct cagcggtggt catatccagc atcagcgaca ggttagagac cgtggataag
     2041 cctgtcccaa cgcccagcaa caccacgccc aagtaaaaca ctccctggct gagggttata
     2101 ccactcacca cgatcaaggc aaagcccagc agggcactca aggcacccca gcgggccaca
     2161 acgcgtttgc ccaaacggcc ttccaggaag ccagccaata acagcgccag caatacacag
     2221 ccaccccaaa gggaggtgat acgcgtcgtg gcctgtaccg acatcccaaa ggcttcgccg
     2281 ccaaaaggct ccaacaggat atcttgccct aagatagctg ccagcaaaac agtcaaatac
     2341 cagaagaaca acctcgcctg tcggttttcc aggatggcgg cgagcatgat gccccaggaa
     2401 taaccttctg aggcggtctg cccgccaaca gatcgccgct ccagccccaa caatcccagc
     2461 gacccgagga ccaaggctgc cagcccgatg atccaaaagg cgcgcgagag ggcttgcggt
     2521 gtgtaggggt ctaccatctg gctcaaggca atcgcagtga cgataatgct ggaaatcatc
     2581 atgaaccaca tcactgcgat ggtcttactg cgtcccttct caccggagag ctccgatgcc
     2641 aatgacaggt aggagacggt agcgaagttg aaacccatac cccacgcccc gaacgtgaca
     2701 agcccaaggc caaagccggc ccaaaagtta ctgctcagca ggaaagccgc ctggggcgag
     2761 agcaccacgc ccagcacgca caggatcagc cccaaggcaa tgtacgggct gcggcgcaat
     2821 cccaagatgg gatggcggtc agagaacgat ccgatagcca tctggatagg cgagaagagg
     2881 tatggcaacg aagccagcaa cgccaccagt gtggccgaga gtgccagctc tttgatcatc
     2941 acccggttga gcgtgctatt gatgggcacc agggtcattg ccacagcgac gtggatcagc
     3001 cccagctgga gacgtttcag cagcattatc gtcctcaatt tattccattt aataattgtg
     3061 aagccagtgc gtccaggctg gaaaaacaaa tccagacaac caataattat gcctcagatt
     3121 atcaggattt acccctcttc gtcaatgtag atttaggtag gttttctata gaaaaaagta
     3181 agcaaatcta caggctttgc acagctcact cggtcaatct gaccacctcg aacacattca
     3241 cctcccaagg gggcaggtcg agatagagac catgcgctaa taggtcatcg ccctggcggt
     3301 cgtagcccgc agagctcaag aggtccttaa gtcgcagggt tgcgccgccc aattcaggga
     3361 aaggcaatcg cacgtaaccc tggctagggt gaggcgcata gttcaccacc gccagccatt
     3421 gccgatcacc cgaaccttgc catgaaaaag cgatcaagct atcccaggaa gcgttattct
     3481 cccaggcagg ggtgcactcc agcagccgcc actcgccatt cctcaatata tcgtcgtgta
     3541 acagttccag taagcgcaaa taaatggcgt ttaccagcct gtctgtgggt tgatctgggg
     3601 cgcggcacag ctggacaggg attttcgtct gccagccctg gatttgaccc tggtggaaaa
     3661 aacgcagtcc cggactcagg taggtcagca cggccgcggc acgatgctcg tcagatgaga
     3721 ataccttcgc ggcgcgcggc tcgtcgtggt tctccaaaaa gcgcaccgac ttattctggt
     3781 aatccagggc agcatcaaaa tgctggcgca cctggcgcgc ctgccgctcc cgcaggcggt
     3841 catatagctg cttgtcatag gtgtaatcaa agccctgctg ttgcagggtc cactccaaat
     3901 cccaatacac ctccgccatg aagacgaaat ccggaaatat tgaacgcact tcctggatcg
     3961 cctgcggcca aaacggagca gccgggatac cccaggtacg ctggaacacc tcgggcagga
     4021 gcaacatcgc catgtcgcag cgcacgccat cgcatagagc cgccactttt gccagctccc
     4081 cgaccacggc ttgtctcaat tcagggttgc cgtaattgag ctgcagggta tccgaccaac
     4141 ccgggaaata tgggtcacga ccgtatgccc ataccggtgc gcctggcgtc tttttcaggc
     4201 gggtgtaatt gtgcggctgc tgggcaagca gctctggcgt gccgtgcaca tagtaatcag
     4261 ggtgcgtctc gacccaggga tggtctggcg cggtgtgatt ggggacaaaa tccagcatca
     4321 ggcgcaagcc gcgctggctc aggcgttttc tgaacctctt cagggcggcg ttaccgccca
     4381 ggtccctgcg caccttgtag ctcttgattg cgaaacacga gccgcaaata tcctgcggac
     4441 gcagatcggg tagggcctca tggtactcac ggagtaactc agggatggat tgcgagatct
     4501 tgcgggcaac cgcgccggtt tgccaaaccc ccaggagata gacccagtca aagcccatgc
     4561 gcgccagctc atccagctcg ctctcgggga tttcatccag cttgcccgag cccccccaac
     4621 gaggcgagcg ggcatacacg cgcgcgttga tctggtatag caacgggtac tttgggtacg
     4681 cctggcggat gctcattgga ttattttacc cgtttgtgcc gttccgaaca cgaatctaag
     4741 atgtgcttta agctcttcgg tgcaaaaacg ccccgcaggt ttccctaaaa ctgctcgtgt
     4801 gatcataaac ctgcgggacg ttcataacca agttatctaa aggtcagtaa ggtttgatcc
     4861 ggtacatgtg gggcggaact caaggttcta cccagacgat gaagctcagc gattggtcgc
     4921 ctgcctgccc tgtgatctcc cagcaaccct cggtggggat atccaccccc accagcatga
     4981 aagagcccag gtccgggttg tagccattgg tcgcaccgga ggctggcaag ggcggcgcag
     5041 aggcgtctag acgctccccg cttacggtta aatccggttg tgggtctgcc tgccaatcat
     5101 agtcttgctg ccaccagatg gtcttctgtg tatagccgtt atcgctgaaa ggcagccccc
     5161 gccaggtgcc atccagcggc agcattgtcc acagcgcatc gctcccgtac caaaatgcgc
     5221 cttcttcggg cgaagagtag gacggtggag gcgcgtacgg ggggtcaggg ggctgcgtga
     5281 cggggcacga accaggctgc ccgcccacgc cggttgccag accggtttga accggcgcat
     5341 tgctatcgcg gatcgcagca cacgcagcga gcgacaggga taggaaaatc gca
//
LOCUS       DBNHOH010000397         3786 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_86450, whole genome shotgun sequence.
ACCESSION   DBNHOH010000397 DBNHOH010000000
VERSION     DBNHOH010000397.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_86450"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="AC16V7_07035"
     CDS             complement(<1..489)
                     /locus_tag="AC16V7_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HIR6256909.1"
                     /translation="MTYSTLFFDLDDTLYPPQNGVWAAIRERMNLYMRDKMRLPSEQI
                     PDLRRYYYETYGTTLRGLQIHYQVDPDEFLAFVHDLPIRKMVQPDPKLRDLILSLPPR
                     KYIFTNADSAHADRVLDALGMQGCFDGKIDVRALDFHCKPEPLAYQTALSLAGETLPQ
                     RCV"
     gene            656..1420
                     /locus_tag="AC16V7_07040"
     CDS             656..1420
                     /locus_tag="AC16V7_07040"
                     /inference="COORDINATES: protein motif:HMM:NF039822.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HIR6256910.1"
                     /translation="MKSIHRFLFPLLALAAIALSVAGVALAREMSERDLFNVSEREIE
                     ITGVVESFVDNTLVIDGQAIVVPVSAEVKGEIVVGQVVKVHALVGDNGTLTAREIEPA
                     MPGMFGDDDLDDDSANINGDDDLDDDLDDDSANINGDDDLDDDLDDDSANINGDDDDD
                     DLFDDVDDDHGANINGDDDDDDDNSGSSSNSGHDDDNDNDNSGHDDDDDNDNSGHDDD
                     DDNDNSGHDDDNDNSGHDDDDDDNDNSGRGDDDDDD"
     gene            1505..2629
                     /gene="mutL"
                     /locus_tag="AC16V7_07045"
     CDS             1505..2629
                     /gene="mutL"
                     /locus_tag="AC16V7_07045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIR6256911.1"
                     /translation="MSIRILASEVASQIAAGEVVERPASVVKELVENALDAGAQRINI
                     TVEEAGRRLIEVADDGSGIPAKELPLAVDRHATSKVSSAEDLIHITTLGFRGEALASI
                     GSVARLSLTTRVQDSAEGAHLRVEGGRMGELQPVGAPVGTLVRVEELFYNVPARLKFL
                     KSDTTERRQIDSLVTRYALAFPRVRFQLRQDGRLSLQTSGDGDRRAVLAALYGVDTAR
                     QMLEVLAEDDNLHIGGFISPVALTRSNRREILFFINRRPVQDPSLTAALVQAYHTMLM
                     VGRYPLAMLFLDMPPEAVDVNVHPTKAEVRFRDTSRMFAAVQRAVRRALLAHTPVPEW
                     KPTSAFPVTGEGFGSGDETTRSAPTIPHPAAPSPYPPPTM"
     gene            2672..3250
                     /locus_tag="AC16V7_07050"
     CDS             2672..3250
                     /locus_tag="AC16V7_07050"
                     /inference="COORDINATES: protein motif:HMM:NF020260.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair MutL family protein"
                     /protein_id="HIR6256912.1"
                     /translation="MVGQVASAYLVAEGPDGLYLIDQHAAHERVLFERMLAQREAVQG
                     KPLPGQTLLEPATVELPPAAARALEDQLPLLGSLGFQVEVFGPGTFLVRAIPALLAGM
                     DATGALRVLVEDFEEDETPLQAEVEARLIARVCKRAAVKAGRTLAPDEQRALLDDLQV
                     CRSPRTCPHGRPTMIHLSVDLLERQFGRRGAR"
     regulatory      3490..3568
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          876 a          943 c         1121 g          846 t
ORIGIN      
        1 aacgcaacgc tgagggagcg tttctcctgc cagggaaaga gccgtttggt aggctaaagg
       61 ctccggtttg cagtggaaat ctaaggcgcg cacgtcgatc ttgccatcga agcagccttg
      121 catgcccagt gcgtccagta ctcgatctgc gtgggccgag tcagcgttgg tgaagatgta
      181 ctttcgcggg gggaggctga ggatcagatc gcgaagcttg gggtcgggtt ggaccatttt
      241 acggataggc agatcgtgaa caaaggctag aaattcatca gggtcaactt ggtagtgaat
      301 ttgaagaccg cgcagggtgg tgccgtaggt ctcataataa tagcggcgga gatcggggat
      361 ttgctcagat ggcaggcgca ttttatcgcg catatagaga ttcatgcgct cccgtatggc
      421 tgcccatacc ccgttttggg gtgggtagag tgtatcgtcc aaatcgaaaa acagcgttga
      481 ataggtcaca gggtaattat aaggcacagg ggaggttagg caaagattaa tagatggtgt
      541 gaatttggtt agccagcgtc acattttgct caaaaatgcg actaataagg tagacaaaca
      601 aacataagat cgctaggtga ggaccaccga gataacacac ataaggagat accaaatgaa
      661 atccatacat cgctttttat tccccctgct tgctctggct gcgattgcgc tcagcgtcgc
      721 tggtgtagcc ctcgctcgcg aaatgtccga aagggacctt ttcaatgtga gcgaaagaga
      781 aatcgaaatt accggagtgg ttgaatcttt tgttgacaac acgctggtaa tcgatggaca
      841 ggccatcgtt gtccctgtct ccgctgaagt taagggcgag atcgtagtgg gtcaggtagt
      901 caaagtgcat gccctggtgg gtgacaatgg cacgttgacc gcccgcgaga tcgagccggc
      961 tatgccaggc atgtttgggg atgatgatct ggatgacgac agtgccaata tcaacggtga
     1021 tgacgacctg gacgatgacc tggatgacga cagtgccaat atcaacggtg atgacgacct
     1081 ggacgatgac ctggatgatg acagtgccaa tatcaacggt gatgacgatg atgacgacct
     1141 ctttgatgat gtagacgacg atcacggcgc caatatcaat ggtgatgatg acgacgatga
     1201 tgataattcc ggcagcagct ccaactctgg gcatgacgac gacaacgata acgacaactc
     1261 aggccatgac gatgacgatg acaacgataa ctctgggcat gatgatgacg acgacaatga
     1321 caattccgga catgacgatg acaatgacaa ctccgggcat gacgatgatg acgatgacaa
     1381 cgacaactct gggcgcggcg atgacgatga tgatgattaa gataaaatag caaacctacc
     1441 caaacccaaa aaaagaatgg ggcggcaaaa ccgccccatt cttttttggc tttataatac
     1501 gggcatgtcc atccgtattc tggcatcaga agtggcctcg cagatcgcgg ctggtgaagt
     1561 tgtggagcgt ccggcatccg tggtgaagga gcttgtcgaa aacgctctgg atgctggggc
     1621 gcagcgtatt aacattaccg ttgaggaggc gggccgacgc ctgatcgagg tggcagacga
     1681 tggcagcggt atacctgcaa aagagctgcc actggcagtg gaccggcatg ccaccagcaa
     1741 ggtgagtagt gctgaagatc taatccacat taccaccctg ggtttccgcg gcgaagccct
     1801 tgcctcgatc ggttcggtgg cgcgcctttc gcttacgacc cgtgtgcagg actcagctga
     1861 gggagcacac ctccgagtag agggaggccg aatgggggag ttgcagcctg tcggcgcgcc
     1921 tgtgggcacg ctggtgcggg tggaagagct tttctacaac gtgccagccc gattgaagtt
     1981 tctcaagagc gacaccaccg agcggcgcca gatcgactct ctggtgaccc gctatgccct
     2041 ggcattcccg cgggttcgtt tccagttgcg ccaggatggg cgtttgtctc tgcagaccag
     2101 cggcgacggc gaccgccggg ctgtgctggc tgccctgtac ggggtggaca ccgcccgcca
     2161 gatgctcgag gttttagcgg aagacgacaa cctgcacatt ggcggcttta tcagccccgt
     2221 agccctgaca cgatctaacc ggcgggagat tttgttcttt atcaatcgcc gtccggttca
     2281 ggatccaagc ttgaccgcag ctcttgtgca ggcctaccac acgatgttaa tggtgggtcg
     2341 ttacccgctg gcaatgctct ttttggatat gccccccgaa gccgtggacg tgaacgtgca
     2401 ccctaccaag gccgaggtgc gcttccgcga taccagccgg atgttcgcgg cggtacagcg
     2461 cgccgtgcgc cgtgcattgc tggcgcatac cccggtccca gagtggaagc cgaccagcgc
     2521 cttcccggtt actggtgaag gttttgggag cggggatgag actacaaggt cagcgccaac
     2581 tatcccgcat ccggcagcgc cctcgccgta tcctccccca actatgtagc cctcacagcc
     2641 gctacccggc acgccacccc cgattttacg cttggttggt caggtagcat cggcctattt
     2701 ggtggctgaa ggtcccgatg gcctatatct gatcgaccag cacgcagccc atgagcgtgt
     2761 cttgtttgaa cgcatgctgg ctcagcgaga agctgtccag ggcaagccgc tgcctggaca
     2821 gaccctgcta gagccggcta cggtggaact gccgccagca gcagcgcgcg cgctggaaga
     2881 tcaattgccc ttgttgggga gcttgggatt tcaggtggag gtatttgggc cgggtacatt
     2941 tctggtgcgc gcaattccgg ctctgcttgc gggaatggac gctacgggag cgctgcgcgt
     3001 gctggtggaa gactttgaag aagacgagac accgttacag gccgaagttg aggcgcgcct
     3061 gatcgctcgg gtatgcaagc gggctgcggt taaagccggc cgcaccctgg cacctgacga
     3121 gcagcgcgcc ttgctggatg acctgcaggt ctgccgctca ccgcgcacat gcccgcatgg
     3181 tcgaccgacc atgatccacc tttcggtgga cttgctagag cgccagtttg gtcgccgggg
     3241 ggcacgctag accgacaaat accatcagga aattatcagg tgtgatctag ggagtttgta
     3301 ataaaataaa ttcatgcgca actgggattg aacttgcgca aaactcagtg ttagggttta
     3361 taataattac attgggatat tagaataagc aagtcaatag ggttgatttt aatctcctag
     3421 caccgatcga ttttattagt agttgtgttt gtcatttaat ggtagtcatg tcaattcgtt
     3481 tggcttgatt cctcgaaaag gcgaacctgc tgcaaagtgg gggcgcaaag tcatgggtct
     3541 gtctcttgca gatcgccagg ctgccgaagg gaataaaacc ttcggcagtt gtcattacag
     3601 aaggaggtga tctcactcat tattcgaccc gtgttttgtg atcaggtgct atacacacaa
     3661 catatttaag gagaaatata gatgaaagcc aagacattcc acatgtttct ggttttaacc
     3721 ttgcttttgc ttttcatggt gcctgcggtg attgccgcgc cagcagcttc ggatggcttc
     3781 gatgac
//
LOCUS       DBNHOH010000398         1786 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_150357, whole genome shotgun sequence.
ACCESSION   DBNHOH010000398 DBNHOH010000000
VERSION     DBNHOH010000398.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_150357"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..>1786
                     /locus_tag="AC16V7_07055"
     CDS             137..>1786
                     /locus_tag="AC16V7_07055"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039222.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043758 - acetate-CoA ligase (ADP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HIR6256913.1"
                     /translation="MLKPFFQPDSVAVIGASSNPSKLGHAVLKNLVDGGYAQRGMVYP
                     INPGANSILGHKAYPSVLDVPAAIDLVVIVIPYPQVPDALRECGQKGIPAAIVISAGF
                     REAGMEGFERERELIQIAKEYNLRLIGPNCLGVIDTFTPLNASFSAGTPPSGPMAFMS
                     QSGALGTAVLDIALAGRLGLSKFVSLGNKADVSEIDLLQAWKDDEHTRVILIYTEGMP
                     NGEEFIRVARQITPKKPVIAIKSGVTQSGSRAVSSHTGSLAGSEQAYQAAFNQAGILR
                     AESMQALFDMALAIGYQPELRGNRIAIITNAGGPGILATDALERAGLSMARFEIETIH
                     ALEQYLPDAASAANPVDVLGDARADRYQFAMEKVIADPNTDGLMIVLTPQAMTDIEAT
                     AEAIGALADATDKPILACFMGEARVEKGIEILNRFGVPNYPFPERAADAFRAMAKYQE
                     IKSRQTSQTQPIEADIKQVQTVITRVRSEKRTTIGDSEARDILQAYGLHIPRSEIASS
                     PERAIEIASQIGYPVVLKIASPDILHKTDVGGVKVGLQNAEA"
BASE COUNT          428 a          512 c          466 g          380 t
ORIGIN      
        1 acggtggaaa ccgagaatat ggccgcgctg aattacgaaa aacctgaccg cgccgcagcc
       61 ataaccctgc aagtcaatcc ccttgacgag cggactatcg tattaatcct gatttccgag
      121 aagtaatata cctgatatgc tgaaaccgtt ctttcaacca gactcggtag ccgtcattgg
      181 agcatcgagt aacccgtcca agttaggcca tgcggtatta aaaaatctag ttgatggtgg
      241 ctacgcgcag cgcgggatgg tttaccccat caatccaggc gctaactcta tcttaggaca
      301 caaagcttac ccctccgtgc tggatgtacc cgctgccatc gatctggtag tgatcgttat
      361 cccctaccct caagtgcccg atgcattgcg tgagtgcggg cagaaaggca tcccggctgc
      421 gattgtgatc agtgccggct tccgtgaagc cggtatggaa ggattcgagc gcgaacgtga
      481 gctcatccaa atcgccaaag agtacaatct gcgcttgatc ggcccgaact gcctgggcgt
      541 tatcgacact ttcacgccac tcaatgcatc cttttctgct ggaacaccgc cttctggtcc
      601 aatggctttc atgtcccagt cgggagcgct tggcactgcg gtgctggata tcgccttggc
      661 gggccggctg gggctctcaa agttcgtcag cctgggaaat aaagccgatg ttagcgaaat
      721 cgatctcctg caagcctgga aagacgatga gcacacccgc gtcattttga tctacactga
      781 aggcatgcct aacggggaag agtttatccg cgtcgcccgc caaatcaccc ccaaaaaacc
      841 ggtcatcgcc atcaagtctg gtgtcaccca atccggttct cgagcggtct catcgcacac
      901 cggctcgcta gccggctcgg agcaagccta tcaggcagct tttaaccagg cgggtatctt
      961 gcgcgccgaa tccatgcaag ccctgttcga catggcctta gcgatcggct accagcctga
     1021 acttaggggc aatcgtattg cgatcataac caacgcgggt ggcccaggca ttttagctac
     1081 cgatgccctc gagcgcgctg gtctgagcat ggcgcgtttc gaaatcgaaa ccatccatgc
     1141 tttggaacaa tacttgccgg atgcagccag cgcggcgaac cccgtagatg tgttgggcga
     1201 tgcgcgagct gaccgctatc aatttgccat ggaaaaagtt atcgctgacc caaacaccga
     1261 tggtcttatg atagtgctca ctccacaggc aatgacagat atcgaggcca ccgccgaagc
     1321 cataggtgcg ctcgctgatg cgacggataa acctatcctg gcatgtttta tgggagaggc
     1381 gcgagtcgag aagggcattg aaatcctcaa tcgcttcggc gttccaaatt atcccttccc
     1441 cgagcgggca gcggacgctt ttcgcgcgat ggccaaatat caggagatca aaagcaggca
     1501 gaccagccaa actcaaccca ttgaagcaga tatcaagcag gtccaaacgg tgatcacccg
     1561 agtccgctcg gagaagcgca caacgatcgg tgattccgag gcgcgtgaca tcctgcaagc
     1621 atatggcttg cacatcccgc gctcagagat tgcctcttca cctgaacgcg ctattgaaat
     1681 tgccagccag atcggctacc ctgtagtgct caaaatcgct tcaccagata tcctgcataa
     1741 gaccgacgtg ggcggcgtaa aggttggtct gcaaaacgct gaagct
//
LOCUS       DBNHOH010000399         2148 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_22727, whole genome shotgun sequence.
ACCESSION   DBNHOH010000399 DBNHOH010000000
VERSION     DBNHOH010000399.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_22727"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="AC16V7_07060"
     CDS             complement(<1..304)
                     /locus_tag="AC16V7_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256914.1"
                     /translation="MSALTIVGLSLFFAFVLAAIFHPQLGLLARWRVNSPAGQKELVE
                     DALKFLLKQEREGRYASPEALAGTLRLYGNDLLRLMTRMETQGLLLSADGGLRLTPD"
     gene            complement(301..969)
                     /locus_tag="AC16V7_07065"
     CDS             complement(301..969)
                     /locus_tag="AC16V7_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HIR6256915.1"
                     /translation="MRHNLTQAIEDYLKAIYEITLSDERATTNQIAERMGVTPASVTG
                     MLKRLATNDPPLLEYRKHRGVELTPAGERVALEIIRHHRLLELFLHEQLGYSWDQVHA
                     EADRLEHVISEDLEERIAQTLGDPRIDPHGDPIPSRELHLPLDTLLPLGELRQGQRAI
                     VQRVRNDDPSLLRYLDSIGLTPQVYLTVLDYSSFDGNLHLQIADRKLVLGPAITQRVY
                     VEVV"
     gene            complement(1051..1641)
                     /locus_tag="AC16V7_07070"
     CDS             complement(1051..1641)
                     /locus_tag="AC16V7_07070"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016107.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR6256916.1"
                     /translation="MNTETPYQLSAIVLLGTAMSISIFFRRRADHLGEKISTDGEGVL
                     ILNLRRIFGLALWLSALAYLLSPHWMAWASLPLSPRVRWSGAMIMAVCVLLIYWVFSS
                     LGKNITSTVAIRKDHALVMHGPYRWVRHPLYTVGFLAFVGFSLLAANWFIFAMLLLGY
                     PVLILRTFTEEANLVARFGDEYRDYMRRTGRFLPRW"
     gene            complement(1645..>2148)
                     /locus_tag="AC16V7_07075"
     CDS             complement(1645..>2148)
                     /locus_tag="AC16V7_07075"
                     /inference="COORDINATES: protein motif:HMM:NF014582.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR6256917.1"
                     /translation="LLDAISRRQSASGKSEASQRLSLAYVIATGIGLHNLGEGLAIGA
                     AYNVGEIALGAFLVVGFIIQNITEGLGIIAPVLRDRPGVGSLALMGLVGGAPAVLGTW
                     IGGFAPNVSLAVLFLAIGTGAVFEVVYEIAKLIQKDTASQPMPMTVFSGVVAGMLLLW
                     VTGLLIK"
BASE COUNT          490 a          641 c          616 g          401 t
ORIGIN      
        1 cgtctggggt caagcgcagc ccgccatctg ccgatagcag caatccctgg gtttccatgc
       61 gggtcatcaa gcgcagcagg tcgtttccat agagccgcaa cgtgcccgcc aacgcttctg
      121 gcgaggcata gcgcccttcg cgctcctgtt tgagcaggaa tttcagggca tcttccacca
      181 gctccttctg tcccgccgga ctgttcaccc gccagcgcgc cagcagccca agctgggggt
      241 ggaaaatcgc cgccagtaca aaagcgaaga acaggctcaa gcccacaatc gtgagggcgc
      301 tcataccacc tccacataca cccgctgggt gatggctggt ccgagcacca acttccggtc
      361 ggcaatctgc aggtgcaggt tgccgtcgaa gctggagtag tccagcacag ttagatacac
      421 ttgcggggtc aatcctatgg aatccaggta gcgcagcaag gaagggtcat cattgcgcac
      481 gcgctggaca atagcccgct gaccctggcg cagctctccc aacggaagca gcgtgtccag
      541 tgggagatgt aattcgcggc tggggatggg gtcgccatgt gggtcgatgc gcgggtctcc
      601 caaggtttgg gcaatacgct cttcgagatc ctccgagatg acgtgctcca ggcggtcggc
      661 ctcggcatgc acctgatccc aagagtaacc caattgctcg tgcaagaaga gctctagtag
      721 gcggtgatgg cggatgatct ccaaggcaac gcgctcgccg gcaggggtga gctccacgcc
      781 gcgatgtttg cggtactcca gcaagggtgg gtcgttggtt gccaggcgct tgagcatacc
      841 cgtcaccgag gcgggagtca cgcccatgcg ctctgcgatc tggttggtag tagcgcgctc
      901 atcggaaagc gtgatttcgt agatagcctt gagataatcc tcgatagcct gggtcaggtt
      961 gtggcgcatc tcagctagtt atcctttcca aacggaacaa catgcgggca gcaatggtga
     1021 tccacttcat tttgattata tgccaatttc ctaccacctc ggcaagaatc gcccggtgcg
     1081 acgcatgtag tctcgatatt cgtcgccgaa cctcgccacc aggtttgctt cctcagtaaa
     1141 ggtgcgcagt atcaacacag gatagcccaa taacagcatg gcaaagatga accagttggc
     1201 tgccagcagg ctaaacccga cgaaggccag aaaccctact gtgtagagcg ggtggcgcac
     1261 ccagcggtag gggccgtgca tcaccaatgc gtgatccttg cgaatagcga ccgtggaggt
     1321 gatgtttttc cccagactgg agaataccca atatataagc agcacgcata ccgccatgat
     1381 catcgcaccg ctccagcgca ccctgggaga cagtggcaag ctagcccagg ccatccaatg
     1441 cgggctgagc agataggcca gcgcagaaag ccacaacgct agaccgaaga tgcgccgcag
     1501 gttgaggatc agcacccctt ctccgtctgt ggagatcttt tcacctaagt ggtctgcccg
     1561 gcggcgaaag aatatggaga tggacatggc agtccccaac aggactatgg cagataattg
     1621 ataaggagtc tctgtgttca tatttcactt gatcaacaac ccggtcaccc agagcaacaa
     1681 catccctgct accacacccg agaagacagt catgggcatg ggctggctag ctgtatcttt
     1741 ttgaatcagc ttggcgatct cgtagaccac ctcaaacaca gcgccggtgc caatcgccag
     1801 gaaaagcacc gccagggaga cgtttggggc gaagccgcca atccaggttc ccaggacagc
     1861 cggcgcgccg ccgaccagcc ccatcaaggc cagcgaaccg accccgggtc tgtcgcgaag
     1921 caccggcgcg atgataccca gcccctcggt gatgttctgg ataataaagc ccaccaccag
     1981 gaatgcaccc agggcgatct caccgacgtt atacgccgca ccgattgcca gcccttcacc
     2041 cagattgtgc aggccgatgc cggtggcgat cacataagcc agagaaaggc gctgggaggc
     2101 ctcgcttttg ccagaagctg actggcggcg ggagatagca tccagcag
//
LOCUS       DBNHOH010000400         1912 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_663903, whole genome shotgun sequence.
ACCESSION   DBNHOH010000400 DBNHOH010000000
VERSION     DBNHOH010000400.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_663903"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..1167)
                     /gene="iolG"
                     /locus_tag="AC16V7_07080"
     CDS             complement(148..1167)
                     /gene="iolG"
                     /locus_tag="AC16V7_07080"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HIR6256918.1"
                     /translation="MDKDRLNIGIVGAGMIGKVHAENLAYRIPEANPRIIADINEKAA
                     WETAARCNIPKMTTDYQEILADSEVDVVVICSSTDTHARIIGEAAQAGKHIFCEKPID
                     FSLANIDQALALVNKAGVKLQIGFNRRFDSNFARVRQAITSGEIGEPHLLHIISRDPE
                     PPTPEYVKTSGGIFMDMTIHDFDMACFLIDSEVEEIFAYGGVRIDPEIGKAGDLDTVL
                     ILLKYADGTLGTIDNSEKAAYGYDQRVEVFGSKGAIQIANNYPNSATISTGESIRREL
                     PLYFFVERYVDSYIAEMRAFVRAVLEDKPTPVSGADGRLPVVMALAARKSYDENRPVS
                     LAEIS"
     gene            complement(1160..>1912)
                     /locus_tag="AC16V7_07085"
     CDS             complement(1160..>1912)
                     /locus_tag="AC16V7_07085"
                     /inference="COORDINATES: protein motif:HMM:NF012742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR6256919.1"
                     /translation="FERRRVDGVVVASSRIGSEYASRLEQIHIPVVMINNQARGDYEN
                     LFSVAVDDYQGAQLAMQHLLDMGHRRIGYIGVQNRPGSNERRMRGYLDALKACGIAAQ
                     PSWVLLGSPQATEDFAGDVEVGQVLMSELAQENVTAVFCYCDTVAAGVMIACRKLGLS
                     VPGQISVVGFDDTDVCTVLAPPLTTISQPRREMGQLAMEMLLTAISGEPTSDVLLQPN
                     LVVRESTAPPNGSSKIQPNSLPADNKNMRSYG"
BASE COUNT          442 a          561 c          479 g          430 t
ORIGIN      
        1 ggctgtactc tggtcagggt cagcaaccgc gaccagatca gcttcaggga cgttaaaagc
       61 cagtgtatgg gcaaagacct tgcccatacg ccctgcgccg atcaacccaa cccggatgct
      121 caaatgctcc ctcccttcac cgtcactcta tgagatttca gctaaactga ccggacggtt
      181 ttcatcataa gatttgcgcg ccgccagcgc catgaccacc ggaagccgcc catctgcccc
      241 gctgactgga gtgggcttat cttcgaggac tgcccgcaca aaggcgcgca tttcggcaat
      301 gtagctatcc acataccgct caacaaagaa atacaagggc aattcgcgac ggatagactc
      361 tcccgtgctg atggtggcgg aattgggata attattggcg atctgtatcg cacccttact
      421 accaaagact tccacgcgct ggtcatagcc atatgccgct ttctcgctgt tatcaatggt
      481 acccagcgta ccgtcggcat acttgagcag gatcagaacg gtgtccaggt cgcctgcttt
      541 tccgatctcc gggtcgatgc gcacgccacc ataggcgaaa atctcctcga cttcactatc
      601 gatcagaaaa cacgccatgt caaaatcatg gatggtcata tccatgaaaa ttcctccgga
      661 agtcttgacg tattctgggg taggcggttc cggatcgcgg ctgatgatgt gcagcaggtg
      721 aggctcgccg atctcaccgc tggtgatagc ctggcgcacg cgggcgaagt tggaatcaaa
      781 acggcggtta aagccaatct gcagcttaac acctgcttta ttcaccagcg cgagcgcctg
      841 atcgatattc gctaaggaga aatcgatcgg tttctcgcaa aagatatgct tgcccgcctg
      901 ggcggcttcg ccaataatcc tggcatgcgt gtcggtcgaa gagcagatga cgacgacatc
      961 aacctccgag tcggcgagta tctcttgata atccgttgtc atttttggga tattgcagcg
     1021 cgctgcagtc tcccaggcag ccttctcgtt gatgtcagca attatccgcg gattcgcttc
     1081 gggaatgcga tacgccaggt tttcggcatg aaccttgccg atcatcccgg ccccaacgat
     1141 tcctatgttt agacgatctt tatccatatg acctcatatt tttattatca gccggtaggc
     1201 tgttgggctg gattttggat gacccatttg gaggcgcggt gctctcacgc accaccaggt
     1261 tgggttgcag aagaacatca ctagttggtt cgccagaaat tgccgtgagc aacatttcca
     1321 tcgccagctg gcccatctct cgccggggct ggctgattgt ggtcagcgga ggggcgagca
     1381 cagtgcacac atcagtatca tcgaagccaa caacgctgat ctggccaggt acagaaagtc
     1441 ccaatttacg acaggctatc atgacgcctg cagccactgt atcgcagtag cagaacaccg
     1501 ccgtgacatt ttcctgagcc agttccgaca tcaagacctg acccacttca acatctccgg
     1561 caaagtcctc ggtcgcctga ggcgatccca gaagcaccca gctgggctga gcagcaatac
     1621 cacatgcctt tagggcatca aggtaaccgc gcatccgccg ctcattcgaa ccagggcgat
     1681 tttggactcc aatgtaacca atgcgccggt gacccatatc caacaggtgc tgcattgcca
     1741 gctgcgctcc ctgataatcg tccaccgcaa ccgaaaacaa gttttcgtaa tcgcctctcg
     1801 cctggttgtt gatcatcacc accggtatgt gtatctgctc caggcgactg gcgtattcac
     1861 taccgatgcg ggacgaggcg acgacaacac catccacacg ccgacgctca aa
//
LOCUS       DBNHOH010000401         3689 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1496920, whole genome shotgun sequence.
ACCESSION   DBNHOH010000401 DBNHOH010000000
VERSION     DBNHOH010000401.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1496920"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="AC16V7_07090"
     CDS             <1..1018
                     /locus_tag="AC16V7_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256920.1"
                     /translation="TMLTFQGMLERILEADGITREMIQAQQTKLNLLQRLLNASEESR
                     IEMARNEDELIDAEFFGMLSRLLETSMASNDRETAQALAELQKSILPETTFGRQLQEQ
                     TQEVEAALSSLQEIGQDLTRDKLLDLVIKAPNDTRVSAFVSLARPVMDYTFFQILSDR
                     IDRARGDGRTRLIQLRERLLDLTRQVDQQLEARAAQSRQLLDNILQAEDVSAAAAQYL
                     PAFDDFFIQELNTALEAARGQGDLEKINDLQRVVQVIQEASSAPPEIAFIEELLDAPD
                     ETTRQKILEDNQDMITPEFLDTLTGLVARVEGGSQDQELVANLKAVHRQSMRYSMQMK
                     FKGQ"
     gene            complement(1019..1687)
                     /locus_tag="AC16V7_07095"
     CDS             complement(1019..1687)
                     /locus_tag="AC16V7_07095"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR6256921.1"
                     /translation="MVIKGEEVPMLMAEAGPLNGQRWPIRGSLIIGRDSDCEIVIPDR
                     QISRRHARITTTPKGVLLEDLGSKNGTYCDGVRVDEPALLQDGTSIQFAFVQTFMFMS
                     SDATLPLENDHLHPVRAETSLLRLELRSRRVWIGNQEILPPLSASQFKLLELLYNNQG
                     KVVTRQELIEAIWGEEQAITVSEQALDALVRRLRERLATFDPSRALIATVRGHGLRLD
                     NPTE"
     gene            1752..2741
                     /locus_tag="AC16V7_07100"
     CDS             1752..2741
                     /locus_tag="AC16V7_07100"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR6256922.1"
                     /translation="MPTPLKAGEVLRNRYKIKRIIGQGGMGSIYLADDLRLEGRLCAL
                     KEVEHDRTMPADLYKQTREQFLREATVLARLDHPNLPKVSDFFSMGGRDYLVMDFVPG
                     KDLRTLMVEARQRGAFLSERDVLGWANQLADALVYMHNQNPPILHRDIKPSNLKLTPN
                     GLVKLVDFGLVKILASDEMTITILQGRGTALYTPLEQYGGDTGHTDARSDIYAFGATL
                     YHLLTNKPPVEARELFLNPDRLIPPRQINPDISLRTDRAILWAMNLHPEERPQDVEAF
                     RQALLGEWNPATRPRAPLPGPSLVDLVSSPTELTMVWISAGLVLLSLIFTLIR"
     gene            complement(2730..>3689)
                     /locus_tag="AC16V7_07105"
     CDS             complement(2730..>3689)
                     /locus_tag="AC16V7_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256923.1"
                     /translation="PHYLDATNIAKLTAYYGLYSKTPNFIDAAARLLFRELRPAGALP
                     ISVLGVGYDLISATMPDPNQVIPLMLDLPSESVTNGTSTPEPVPTPEFRVESLIAVRT
                     GVIIDHNGHPVPDGTPVQFIATTNGEVNTLPQIVTTQDGIAFTTIQVNASGSLEIRAE
                     SEPAKQSQILHFDIPAINGDITPTATEMPTETPTTMPNPTILPTQMATQEPENLVSPN
                     FRDWIMAILLTSIVGALSYRLAAYIGQVRWGVRSGFLSLIGGLLAYTYLALGAPGSQN
                     LLETAGSWGIVLVTLTGCTAGILAAWSWRAIQSALKSESRSAN"
BASE COUNT          899 a          885 c          985 g          920 t
ORIGIN      
        1 gaccatgctg accttccagg gaatgctgga gcgcatcctg gaagctgatg ggatcacgcg
       61 tgagatgatc caggctcaac agacgaaatt gaacctgttg cagcgtcttc tgaacgcctc
      121 tgaagagtct cgcattgaga tggcacgtaa cgaagacgaa ctgatcgacg cagagttctt
      181 tggaatgctt agccggttgc ttgagacatc catggcttct aatgatcgcg agacagccca
      241 agctttagct gagcttcaaa agagcatatt gccggagaca acctttggtc gccagttgca
      301 agagcaaact caagaggtgg aggcggctct atcttcattg caagaaattg ggcaggattt
      361 aacgcgggat aaattgttgg atctggtgat aaaggctccc aatgatacgc gcgtgagtgc
      421 tttcgtcagc cttgccagac cagttatgga ttatacgttc ttccaaatcc tcagcgatcg
      481 tatcgacaga gcgcgcggag atggacggac ccgtttaatt caattgcgcg agcgtctgct
      541 agatttaacc cgtcaggtgg accagcagct tgaagcccgc gctgctcagt ctcgtcagtt
      601 gctagataat attctgcaag ctgaagatgt tagcgcggcg gcggcgcaat acctgcctgc
      661 atttgatgac ttctttatcc aggagttgaa cacggctctt gaagctgcac gtgggcaggg
      721 cgatttagag aaaatcaatg atttgcaaag ggttgtgcag gtcatccaag aagcatcctc
      781 tgcacctcca gagatcgctt tcatcgagga attgcttgac gcgccagacg agactacccg
      841 ccagaaaatc ctagaggata atcaggatat gattacaccc gaattcttgg atacgctcac
      901 gggactcgtc gcacgggtgg aaggtggcag tcaggatcag gagctggtgg caaatctcaa
      961 ggctgtccac cgccaatcca tgcgctactc gatgcagatg aaatttaaag ggcagtgatt
     1021 attctgtagg gttgtcgagt cgaagaccgt gaccgcgaac cgtggctatc aaggcgcgag
     1081 atggatcaaa agtggcaaga cgttcacgca atcgtcgtac gagggcgtct agagcttgct
     1141 cggagacagt gatcgcctgt tcttctcccc agatggcttc gatgagctcc tggcgggtca
     1201 ctacttttcc ttgattattg taaagcaact ctaataattt aaattgagag gctgataggg
     1261 gtggcagaat ttcctggttg cctatccaca ccctccttga gcgcagctcc agtcgcagta
     1321 agctagtctc ggctctcaca ggatgaaggt gatcattctc aagaggtaaa gtggcatctg
     1381 agctcatgaa catgaaggtt tgtacaaaag cgaattggat actggttcca tcttgcagca
     1441 atgctggttc atcaactcgc actccatcgc aatatgtacc gtttttgctg cccagatctt
     1501 ccaggagtac tcctttgggc gttgtggtga tgcgggcgtg acgtcgtgag atttgccgat
     1561 caggaatcac gatttcacaa tctgaatctc gcccgatgat tagactgcca cgaataggcc
     1621 agcgctgacc attcaatggt ccagcttcag ccattaacat tgggacttct tcacctttta
     1681 ttaccatgtc cctgcctcca cgggtataat atcatactgt gggcatctat agataatctt
     1741 aatttcaaat catgccaacg ccgctaaaag caggagaagt attaagaaat cgctacaaaa
     1801 taaaacggat cataggccaa gggggtatgg gaagcatcta tttagcagat gatctgcggc
     1861 tggaagggcg gttgtgtgcg ctcaaggagg tcgaacacga ccgcaccatg ccagccgatc
     1921 tatacaagca aacccgcgag cagttcctgc gcgaggctac agtattagcg cgcctggatc
     1981 atcccaacct gcccaaggtc tccgattttt tctcaatggg cggtcgtgat tacttggtaa
     2041 tggactttgt tccgggaaag gatctgcgca cattgatggt cgaagcccgc cagcgggggg
     2101 catttttatc cgagcgtgac gtgctcggtt gggcaaatca attagctgac gcgttggttt
     2161 acatgcataa ccaaaacccc ccaatcctgc accgggatat taaacctagc aatttaaaac
     2221 tgacacccaa tggtctggtt aagctggtag attttggatt ggtgaaaatc ttagcgtccg
     2281 acgaaatgac cattacgata ttgcagggac gtggcaccgc attgtatacc ccattggagc
     2341 aatacggtgg ggatactggc catactgacg cccgcagcga tatatacgca tttggggcaa
     2401 cactatacca tctgctaacc aacaagccac ctgtggaagc gcgcgaatta tttctaaatc
     2461 cagatcggct tatcccacct cgccagatta acccagatat cagcttgcgc acagatagag
     2521 cgattctttg ggcgatgaat cttcatccgg aggaacgtcc tcaagatgtg gaagccttcc
     2581 gccaggcact tttaggcgag tggaacccag ccacccggcc gcgtgcccct cttccaggac
     2641 cgtctctggt agatttggta tcctctccca ccgaattgac aatggtgtgg atttcggcag
     2701 gcctggtttt gttgagtctg atttttacgc taattcgctg atctgctctc ggattttaat
     2761 gctgattgga ttgcccgcca actccaggca gctaatattc ccgcagtgca tccggtaaga
     2821 gtaaccaaga caattcccca gcttccagct gtttccaata gattttggct gccaggcgcg
     2881 cctagggcga gataggtgta ggctagcagg ccaccgatta aagataaaaa tccgctacga
     2941 acgccccatc tgacctgacc aatataggcg gccaatcgat acgataatgc acccacaata
     3001 gacgtcaaaa ggatggccat gatccaatct cggaaattgg ggctgaccaa attttcgggt
     3061 tcttgggttg ccatttgggt tggtagtatt gttgggttgg gcatggttgt gggggtttca
     3121 gtaggcatct cagttgcagt gggagttata tctccattta ttgctggtat atcgaagtgt
     3181 aatatttgcg attgtttggc tggttcactt tcagcgcgaa tttccagcga ccccgaggca
     3241 ttcacctgaa tggtggtaaa ggcgataccg tcctgggttg ttacgatttg gggtaatgta
     3301 ttgacttcac cgttggtggt tgcgatgaac tgtacaggtg tcccatccgg cactgggtga
     3361 ccgttgtgat cgatgatcac tccagtgcgc actgctatca ggctttctac ccgaaactcc
     3421 ggagttggga caggttctgg cgtactggtt ccattggtga cagattccga agggagatcg
     3481 agcatgagtg ggattacctg gttagggtcc ggcatggtcg cagatatcag atcataaccc
     3541 acacccagaa ctgaaattgg caaggctccc gccggacgga gctcgcggaa gagcaggcgg
     3601 gccgctgcgt cgatgaaatt tggggttttg ctgtacagcc cgtaataggc agtgagcttg
     3661 gcgatattgg ttgcatctaa atagtgcgg
//
LOCUS       DBNHOH010000402         3085 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_86740, whole genome shotgun sequence.
ACCESSION   DBNHOH010000402 DBNHOH010000000
VERSION     DBNHOH010000402.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_86740"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..704)
                     /locus_tag="AC16V7_07110"
     CDS             complement(177..704)
                     /locus_tag="AC16V7_07110"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HIR6256924.1"
                     /translation="MEILLSSQAWLGAGLIFILRVTDMTLDTLRVLVVMRGRKSYAWI
                     LGFFQAGIFVVAISTVLQNLDNPLNLVGYASGFATGNVVGLLIEQRLAIGHILLNIVS
                     PSRGTAIAEYLRGEGYAVTEIPARGKDGMVSLLYCNVLRRNVDRVRKLVNEVDPEAFI
                     TSEDVRPVMRGFWRA"
     gene            complement(708..2885)
                     /gene="glgP"
                     /locus_tag="AC16V7_07115"
     CDS             complement(708..2885)
                     /gene="glgP"
                     /locus_tag="AC16V7_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HIR6256925.1"
                     /translation="MESFRTVIPNQFSLPRRIARLGDLAYNLWWTWDPDSQRLYSGID
                     RELWDRLNHNPVLFLRQVERARLNAVTNNRHYLEYYDRIFRAFDEYMQAGETWFSSHN
                     PELRNRPIAYFSMEFGLHEIFPIYAGGLGVLSGDHVKEASDLGLPFVAVGLYYSEGYF
                     TQRIAEDGWQEASYTPHALEELPIQAVLDTEDMPVTLSVELPGREVIARVWEIRVGRV
                     PVYLLDTNVPENIPGDRALSARLYSSDPEVRISPEILLGMGGLRALRVLGYNPSVWHM
                     NEGHSAFLILERTRELVNSGYDFEKAKEMVRASNVFTTHTPVPAGNDEFPLWLTDKYF
                     AHIWPELGLSRDQFIDLARHSVSWGDSFSMPMLALRFSQGCNAVSELHGRVARKMWNF
                     VWPQRREDDVPITHITNGIHTGTWLARRMGHLFDRHLGQDWRERVDDPDVWDQILAIP
                     DDELWEVRRHLKRKLAYYITDCARNRWVKGGAPPIQILAGGALIDPNVLTIGFARRFA
                     PYKRANLILRDIDRLLTSLNRPGKPIQIIFAGKSHPDHEGGKMLIQDVYRAVKRPEAG
                     GRLVFLEEYNMNLARYLVQGVDIWLNTPRRPNEASGTSGQKAALNGALNFSVLDGWWR
                     EGYNGKNGWAIGEDRDYDNLDQQDEIDAESLYDILEDEIIPLYYDERTADGLPFEWIA
                     RVKESVRTLAPLFSMRRMVKEYLEDMYLPGMQSSPSTQKQKGS"
BASE COUNT          786 a          856 c          741 g          702 t
ORIGIN      
        1 tcaacaaatt agcagtcagc gattattaat cactaacccc aatatcgccc tataggctgg
       61 ctgtacttat tattgcagat ttctcaaaaa tgtctatatg tgatcgagaa tcaaaatgtc
      121 catactgccc acctgtgaga tcgcaggcca aagaggagtc aaagcggaag ggaaaactat
      181 gcccgccaga agcctcgcat cactgggcgc acgtcttccg acgtgataaa tgcctcgggg
      241 tcgacttcgt ttaccagctt cctcacccga tccacattgc gccgcagcac attgcagtac
      301 agcaggctga ccatgccgtc cttgccgcgc gccgggattt cagtcaccgc gtatccttct
      361 ccgcgcagat actcggcaat ggctgtgcca cggctggggc tgacgatgtt cagcaagatg
      421 tgcccaatcg caaggcgctg ctcgatcagc aatccaacca catttcccgt ggcgaaccca
      481 gacgcgtatc cgaccagatt caacgggtta tccaagttct gcaatacagt gcttatcgcc
      541 acaacgaaga tacccgcctg aaaaaaaccc aatatccacg cataggattt acgaccacgc
      601 ataaccacca gcacccgcag cgtgtccagg gtcatatcgg ttacgcgcaa aatgaagatc
      661 agacctgcac ctaaccaggc ctgagatgaa agtagaattt ccattaacta cgatcctttt
      721 tgtttctgag tcgaggggga ggattgcatg cccggtaagt acatgtcttc cagatattcc
      781 ttcaccatgc ggcgcatact aaaaagcggt gccaatgtgc gcacagattc cttgactctt
      841 gctatccact cgaacggtaa gccatcagcc gtgcgttcgt cataatagag cgggataatt
      901 tcatcctcca ggatgtcata caggctctcg gcatcgattt catcctgctg gtcaaggttg
      961 tcatagtctc ggtcttcgcc aatggcccaa ccgtttttgc cgttatagcc ttcccgccac
     1021 cagccatcca gaacggaaaa gttcaatgcc ccgttcaagg cggctttttg cccggaggtt
     1081 ccagaagctt cattgggacg gcgcggtgta ttcagccata tatccactcc ttgcaccagg
     1141 taacgcgcca gattcatgtt gtactcttct aaaaagacca ggcgccctcc agcctctggg
     1201 cgctttacag cgcggtaaac atcctggatt aacatcttgc cgccttcatg atcagggtgt
     1261 gatttgccag caaagataat ttgaatcggc ttgcccggac ggttgagcga tgtgagcaag
     1321 cggtcgatat cgcgcaagat caggttggcg cgtttatagg gtgcaaaacg tcgtgcaaaa
     1381 cctatggtga gcacattcgg gtcgatcaat gcgccgcctg ccagaatctg gatcggagga
     1441 gcgcctcctt tcacccaccg gttgcgggcg cagtcggtga tgtaatacgc cagtttgcgc
     1501 tttaagtgcc tgcgcacctc ccaaagttca tcatcgggga tagcgagtat ttgatcccac
     1561 acgtctggat catcaacgcg ctctcgccaa tcctgaccca ggtgacgatc gaacaaatgc
     1621 cccatacgcc tggctagcca ggtgccggta tggatgccgt tggtaatgtg ggtgattggg
     1681 acatcatctt cacgccgctg aggccataca aagttccaca tcttgcgcgc cactctaccg
     1741 tgaagttcgg acacagcgtt gcaaccctgc gaaaaacgca acgccagcat cggcatgctg
     1801 aagctatctc cccaggaaac tgaatggcga gccaaatcga tgaactggtc gcggctgaga
     1861 ccgagttcag gccaaatatg agcgaaatat ttatccgtta accacagcgg gaactcgtca
     1921 tttcctgccg gaaccggtgt gtgcgtagtg aacacgttgg atgcgcgcac catctccttg
     1981 gctttctcaa aatcgtatcc cgagttgacc aattctcgcg tacgctccag tatcaagaaa
     2041 gcagagtgcc cttcgttcat gtgccatacg ctggggttat aacccagcac tcgcaacgcc
     2101 ctcagacctc ccatgccaag caatatctcc ggagaaatac gcacttcagg gtcactgctg
     2161 tacaagcgag cagataaagc gcggtcgccg ggaatatttt ccggcacatt ggtatctagc
     2221 aggtacaccg gcaccctacc cacacgaatt tcccacaccc gggcaattac ctcgcggccg
     2281 ggaagctcaa ccgatagggt aaccggcata tcctcagtat ccagaacagc ctggataggc
     2341 agttcctcca gtgcgtgcgg agtataggaa gcctcctgcc agccatcctc ggcgattctc
     2401 tgggtaaagt aaccctcgga ataatacaag cctaccgcta caaagggtag gcctaaatcg
     2461 ctggcttctt tgacgtggtc accagataga acacccaatc cacctgcata gatcggaaag
     2521 atctcatgca ggccaaactc cattgaaaaa taggcgatag gacggttgcg caactccgga
     2581 ttatggctgc taaaccaggt ttcgcctgcc tgcatatact catcaaaggc gcgaaaaatg
     2641 cgatcgtaat attccaggta atgacggttg ttggtaaccg cgtttaatct tgcccgctcc
     2701 acttgcctga gaaagagcac aggattatgg tttagccgat cccataactc tctgtctatt
     2761 ccagaataca gacgctggga atcaggatcc catgtccacc ataggttata agccagatca
     2821 cccaaacgag cgatgcgtcg aggcaggcta aattggttcg gaattacagt tcgaaatgat
     2881 tccatgaatt acgaaatgct ccttatatag cgtggatgtg cgagcgaata atattaccac
     2941 atcaaggggc actctcacac tcgaggcgat taaacatact tgtgattccc gcgttaaaat
     3001 cgtttgaggg tgtagaattt gcaagaatgc cctcccaaaa gcgacatatt cgaggtgaga
     3061 gaaatcggtt tacttgacgt acatg
//
LOCUS       DBNHOH010000403         1257 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_920782, whole genome shotgun sequence.
ACCESSION   DBNHOH010000403 DBNHOH010000000
VERSION     DBNHOH010000403.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_920782"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="AC16V7_07120"
     CDS             complement(<1..1008)
                     /locus_tag="AC16V7_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HIR6256926.1"
                     /translation="MKRPMVTIDGNEATAFVAHRVSEVIAIYPITPSSSMGEHADKWS
                     AINHKNIWGTVPLVVEMQSEGGAAGTVHGALQAGARTTTFTASQGLLLMIPNMYKIAG
                     ELTSTVFHVSARTVATHALSIFGDHSDVMATRSTGFAMLASNSVQEAQDLALIAHAAT
                     LEARVPFVHFFDGFRTSHEVNKIEQLLEEDIRAMIDDELVHAHRARALTPDHPFVRGT
                     AQNPDVFFQARETINPYYQVCPDIVQKAMDRFASVVGRQYKLFDYVGAPDAERVIVIM
                     GSGAETAGETAKFLQENGESVGVLKVRLYRPFSVKYFLEALPPSVRAIAVLDRTKEPG
                     AA"
BASE COUNT          312 a          311 c          322 g          312 t
ORIGIN      
        1 agctgcgcct ggctctttgg ttcgatctaa aaccgcgatg gcgcgcaccg aaggcggcaa
       61 ggcctccaag aaatacttca ccgagaacgg acggtatagg cgtactttga gcacaccaac
      121 actctcacca ttttcttgca ggaacttggc tgtctcgcct gcggtctctg cgccggagcc
      181 catgatgacg attacccgct cagcgtcagg tgcaccgacg taatcaaaga gtttatattg
      241 tcgcccaacc acactggcga aacggtccat cgctttctga acgatatcag ggcaaacctg
      301 gtaataagga tttatcgtct cacgtgcctg gaagaacaca tctggatttt gggctgtgcc
      361 tcgcacgaag ggatggtcag gagtgagagc gcgtgcccgg tgagcgtgca ctagctcatc
      421 gtcaatcatc gcccggatat cttcctcgag cagttgttcg attttattga cctcgtggga
      481 tgtgcggaag ccatcgaaga aatgcacgaa aggcacgcgc gcttccagag tagctgcatg
      541 agcaatcagg gctagatcct gtgcctcctg cacagaattg gatgcaagca tagcgaagcc
      601 agtgctccgt gttgccatta catcggaatg atctccgaag atcgataggg cgtgggttgc
      661 aacggtacgc gccgagacgt ggaaaaccgt agacgtcaat tcgccagcga tcttatacat
      721 attcgggatc atcaaaagca gcccctgaga tgcggtgaac gtagtagtcc gcgcaccagc
      781 ctgcaacgca ccgtggactg ttccagctgc accaccctca gattgcattt ctacaaccag
      841 aggcacggtg ccccagatat ttttgtgatt aatcgccgac cacttgtcgg cgtgctcacc
      901 catcgatgaa gagggcgtaa tagggtagat cgcgataacc tcactcactc gatgtgctac
      961 aaatgctgtg gcttcattac cgtcaatagt aaccataggt cgtttcatga attcgctcca
     1021 aatctgcctg cccaggttgg gtaggtcgac aatgagaata gttgttagtt tcaatttaac
     1081 agtttagtcc cgcttcgaat tttcacatag ttattttagg catgtaaaac cctgagacct
     1141 gtcatatttc agacaatttt aggtgttatt aagtagagtg tatgtgatca gaaaattaaa
     1201 tccttgattg tgtaattgct tcataagtta cgcagttcga gaggtgatgg gggagtt
//
LOCUS       DBNHOH010000404         3029 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_856260, whole genome shotgun sequence.
ACCESSION   DBNHOH010000404 DBNHOH010000000
VERSION     DBNHOH010000404.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_856260"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1656)
                     /locus_tag="AC16V7_07125"
     CDS             complement(<1..1656)
                     /locus_tag="AC16V7_07125"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6256927.1"
                     /translation="MAASTPALIQIDEKEYSRRIWAWTMYDWANSAFATTILAAVLPI
                     YFSQVAGANLPSAATATAYWSTGLSVSLLIIAVLSPILGTISDVMRGKKPFLAVFAGI
                     GIVSTALLILVGTGDWVLASVFGVLGRIGFSGANTFYDALLPHVAREADRDKVSARGY
                     AMGYLGGGLLLAINIVMIQMLPGTWGARLSFFSVAIWWAVFSIPLFLRVPEPPAATAK
                     LDSGENVISVSFKRLAETLRDIRQYRELFKFLLGFLIYNDGIGTIIGVAAIYGAELGF
                     GSLELILALLLVQFVGIPYSLIFGRLPSQNEKRRPFFLAYVLFNLVALPLVGIAGTRL
                     LPSQITGTPPARFASSPTAAGQGIYLATDNRLKTTGTWSDLEVSAAELGGETSATYRA
                     GSDAEARASFTFNGEKVELTYSSGPQGGIWAVLMDGQPLIDPDSGEPLTIDAYHPTVR
                     YGEEVVLAAAAPGEHTLVLANTDRKAPESQDTQISLAQIEVLPPSRQSNLLVIIGLIA
                     AVEIIGLLISWVLGPPLFSRLAASIDTRRAILLALSVYAVISVW"
     gene            2065..>3029
                     /locus_tag="AC16V7_07130"
     CDS             2065..>3029
                     /locus_tag="AC16V7_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HIR6256928.1"
                     /translation="MSVEFIFRLVGMVVFAIMGAYFGVYLAAISNGPIDMYGVVVALT
                     GALAGLILTPYLTTRPARYMRAVFTRISAQTLAASLTGLIAGLIIAALLAFPFSMLPD
                     PFRAILPFIGVLVFGYLGVAVFVMRQNDIFSILRINLPGRSAPGNDGGANRDASRTIL
                     LDTSVIIDGRIADITRTGFLVGTLMIPRFVLNELQYIADSADGLRRQRGRRGIEVLSQ
                     LQKDTTIPVRISDIDIEGTREVDDKLVILARQLRCPILTNDYNLNRVAELQGVPVLNV
                     NELANAVKSVLLPGEALAVNIIQEGKESGQGVGYLDDGTMVVVEDG"
BASE COUNT          686 a          769 c          856 g          718 t
ORIGIN      
        1 ccagacggag atcacggcat agaccgacag ggcgagcagg atagcccgac gggtgtcaat
       61 gctcgccgcc aagcgggaga agaggggcgg acctaaaacc caggaaatca gcagtccaat
      121 tatctcgacc gctgcgatca ggccaatgat caccaaaagg ttgctttgac gagagggtgg
      181 taacacctca atttgggcca ggcttatttg tgtatcttgg ctctccggag cttttctgtc
      241 ggtattggcc agcaccaggg tatgctcgcc cggcgcggct gctgcaagta ctacctcttc
      301 gccatagcgc actgtcgggt ggtaggcgtc tattgtcagg ggctccccgc tgtctgggtc
      361 gatcagcggc tgcccatcca ttagaaccgc ccagattccg ccctgaggac cactgctgta
      421 agtgagttcg accttttcgc cgttgaaggt gaagctggct cttgcctctg cgtcagaccc
      481 ggcgcggtaa gtggcgctgg tctcacctcc cagctccgct gcgctcactt ctaggtcact
      541 ccatgtgccg gtcgtcttca ggcggttgtc ggtggctaag taaataccct gtccggcggc
      601 tgttgggctg ctggcaaagc gggcaggtgg cgtgccggtg atctgggatg gcaggaggcg
      661 cgtgccagcg atccctacca agggcagggc aaccaggttg aaaagcacat atgccaggaa
      721 aaacggccga cgtttctcgt tttgactggg tagccggcca aagatcaggc tgtaggggat
      781 gcccacaaat tgcaccagca gcaatgccag gatcagctcc aggctgccaa agcccagctc
      841 ggcgccatag atagctgcta cgccaatgat agtgccgatg ccatcattgt aaattaagaa
      901 gcccagtaaa aacttaaaca gctcgcggta ttggcggatg tctctcagtg tctctgccaa
      961 gcgcttgaag ctgactgaaa tcacattttc acctgagtcc agcttggctg tggcggctgg
     1021 cggctcaggc acgcgcagga aaagcgggat agagaagact gcccaccaaa tcgccacgct
     1081 gaagaaggac aggcgcgctc cccaggtgcc gggcagcatc tggatcatga caatattgat
     1141 tgccagcaac agcccgccgc ccagataacc catggcgtag ccgcgcgccg aaactttgtc
     1201 ccggtctgcc tcgcgcgcca catgaggcag taaggcatca tagaacgtgt tggctccgct
     1261 aaaaccgatg cgccccaaga cgccgaagac ggaggctaat acccaatccc ccgtcccgac
     1321 cagtatcagc aacgcagtgc tgacgatccc gattccggcg aaaaccgcca ggaaaggctt
     1381 cttaccgcgc atcacatcgc tgatggtgcc caggatgggc gagagcacag cgatgatcag
     1441 cagggaaaca ctcagcccag tgctccagta cgctgtggcg gtggccgcgc tgggcaggtt
     1501 cgcgccggcg acttggctga aatagatggg tagcacagca gccagaatcg tggttgcgaa
     1561 agcggagttg gcccagtcgt acattgtcca ggcccaaata cgccgagagt attctttttc
     1621 gtcaatttgg attagggcag gggtactggc agccatcgtg gaatctccca atagataaga
     1681 tgccagcaat tatagccgac actgcaacaa tacattgata ctactcaccc ccaatttttt
     1741 agagaagacg ggctggaaaa acgcaatcat tgtctttttt ggaaattccc ccaataataa
     1801 gcccaataga taagtttttt gaattataat gagacacata ttaattcatt tggcgatcca
     1861 ctccggcgat aattattttc tttgattatt ctttacctgc gcgaagatgc cggattggtt
     1921 gctgcattat tcaaggactt attttttatt atctacagta agtagcttga taatagcgac
     1981 cttgggttgt cacgcctatt gtgacaggcg gacaggtgtg agcgcaggta tattgcgctc
     2041 tttttgtttt tataaggagt tattatgagc gttgaattta tctttcgcct ggtcggtatg
     2101 gtagtgtttg caatcatggg tgcttatttt ggggtatatt tggcggcgat ctcgaatgga
     2161 ccgatcgata tgtatggcgt ggtggtggcg ttaaccggag cgttggcagg tctgatcttg
     2221 acaccatact tgaccacgcg tccggcgcga tatatgcggg cagtgttcac gcgcatctcc
     2281 gcgcagactc tggcggcaag tctgacgggg ttaattgcag ggttgatcat tgctgccttg
     2341 ctggctttcc ctttctcaat gctacctgac ccatttcgtg caatcctgcc atttatcggc
     2401 gtgctcgttt ttggctatct cggtgtagcg gtatttgtta tgcgccagaa tgatatcttc
     2461 tcgattctga ggatcaacct tcctgggcgc tctgcgccag gaaatgatgg cggcgcaaat
     2521 cgtgatgcct cacgcaccat tttgttagat accagcgtga ttatcgacgg tcgtattgct
     2581 gatatcaccc gcacaggttt cctggtaggc acgcttatga tcccgagatt tgtgctcaat
     2641 gaattgcaat acatcgctga ctcagccgac ggtttgcgta ggcagcgagg ccggcgtggg
     2701 attgaggtgc tatctcaatt gcagaaagac acaacgatcc ccgttcgcat cagcgatatt
     2761 gatatcgaag gaacgcgaga ggtggatgat aagctggtca tccttgcacg ccaattgcgt
     2821 tgtcctatct tgaccaatga ctataatcta aatcgtgtgg ctgaactgca aggtgttccg
     2881 gtgctgaatg ttaacgaatt ggcaaatgca gtgaaatcgg tgctgctgcc gggcgaagcg
     2941 ctggcggtga atatcatcca agaaggtaaa gagagcgggc aaggcgtagg ttacctggac
     3001 gatggcacca tggtggtggt ggaagatgg
//
LOCUS       DBNHOH010000405         2175 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_792262, whole genome shotgun sequence.
ACCESSION   DBNHOH010000405 DBNHOH010000000
VERSION     DBNHOH010000405.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_792262"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="AC16V7_07135"
     CDS             complement(<1..418)
                     /locus_tag="AC16V7_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR6256929.1"
                     /translation="MRKIAWIISIFVIAAVLLAACGGPQATEAPPPTEAPPPTEAPPE
                     GGIPDLGGQTIAVAVENAYPPFNTIDEVTNEGVGWDYDAVREICARLNCVPEFVQAAW
                     DGIFPAMQAGEFDMLADGVTITAERDEIVDFSIPYVI"
     gene            630..1034
                     /locus_tag="AC16V7_07140"
     CDS             630..1034
                     /locus_tag="AC16V7_07140"
                     /inference="COORDINATES: protein motif:HMM:NF014132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HIR6256930.1"
                     /translation="MPHFPSPEWLPSLKDKLNTDERYAQIAQNWEGDMLVFIEPDGAL
                     TQPFAYYLDLWHGKCREVYIVDQNQEVKPFLTLSGRYGIIVRVIKGELDPIQAMLTRK
                     LHVEGNMIVLVRNVPTVMEFIRCCRENTDSYT"
     gene            1098..>2175
                     /locus_tag="AC16V7_07145"
     CDS             1098..>2175
                     /locus_tag="AC16V7_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256931.1"
                     /translation="MQSQTPNRSPSQRRAGMKSRLTLVLFILFSAGIFFVTASIGVLG
                     GYQTGMREYSRLQSIDEVKFLQEQYLLAVQDFDSERYDLARQRFEYVLEQDPAFPGAA
                     AKLVQVVQILYATATPTAIPPTVTPTPTRDLRPVQDQFNQAQVDYSQGNWNSAIDVLL
                     GLRKEDAAYNVVEVDSLLYRSLRNRGLQKIREEANLEGGMYDLSLAENFGPLDTQADN
                     VRNLARLYVIGSGFWEVYPEQAVFYFSQVAAAVPGLRDASGWSASGRLWASLVQFGDF
                     LAERDDWCAAEEKYNAAFAMQSNTELMEKISQAYDKCHPPTETPKPTPKPSETATNTI
                     VPPVITVETPTSTTLPPAVTQTATR"
BASE COUNT          538 a          509 c          570 g          558 t
ORIGIN      
        1 cgatgacata ggggatagag aagtcgacga tttcgtcgcg ctcggcggtg atggtgacgc
       61 catcggcgag catatcgaac tcaccagcct gcatagcggg gaagatgcca tcccaggcgg
      121 cttgtacgaa ctcgggaaca cagttgaggc gagcgcagat ctcgcgcacg gcgtcatagt
      181 cccagcctac gccttcattg gtgacctcat cgatggtatt gaagggcggg taggcgttct
      241 ccacagctac tgcgatagtc tgacctccca ggtcagggat tccaccttct ggcggcgctt
      301 cagtcggtgg cggtgcctcg gtaggcggcg gtgcttcagt agcttgaggc cctccacagg
      361 ctgccaatag tacggctgcg atcacaaata tggaaattat ccaggcgatt tttctcattg
      421 ctgtctcctt attatattat tttcagtgat gttaaacatg ttgtgttttg tcatgtgcct
      481 cctctgttag tagcttggaa tttggttaga atacaagaaa attgcgaata gagcaattga
      541 aaacagtata atcataatac gtattcactt agttgaaaag ttgctcgttt attatttata
      601 aagttgttat atagagagag taaaaccaaa tgccccattt tccaagtcca gaatggctgc
      661 ccagcctgaa agataagttg aacacggatg agcgttatgc acaaatagcc caaaattggg
      721 aaggagatat gcttgtattt atcgaaccag atggcgctct tacccaacca tttgcttatt
      781 atttagattt atggcacggc aagtgtcgag aagtttatat cgtggaccaa aatcaggagg
      841 tcaagccgtt cttgacgctc agcgggcgat atgggattat tgttcgcgtc atcaagggcg
      901 aactcgaccc gattcaagcg atgctcactc gtaagcttca cgttgagggg aatatgattg
      961 tcctcgtgcg caatgtgccc acagtgatgg agtttatacg ttgctgccgg gaaaatacgg
     1021 atagctatac ctaattggga acgtcgatgc cgcaatttgc gtctataagg ggcgagctct
     1081 caactcgtta ttgatctatg caatctcaaa ctccaaatcg ctctccttcg caacgtcggg
     1141 ctggcatgaa atccaggttg actttggtgc tatttattct attctcggct ggaattttct
     1201 tcgtgacagc atccattggg gtgttggggg gataccaaac cggcatgcga gagtatagca
     1261 ggttgcaatc cattgatgag gttaaatttc tccaagagca gtatctcttg gcggtacagg
     1321 attttgattc ggagcgttat gacctggctc gccaacgctt tgaatatgtc ttggaacaag
     1381 atccggcttt ccctggagca gcggcaaagc tggttcaggt ggtgcagatc ttgtacgcga
     1441 ctgccacccc caccgccatc ccgccgacag tcaccccaac gccgacgcgt gatttgaggc
     1501 ctgtgcaaga tcagtttaac caagctcaag tcgattattc ccaaggaaat tggaacagcg
     1561 cgatcgatgt tttattggga ttgagaaaag aagatgcagc ttataacgtg gttgaggtcg
     1621 atagcctgct atatcgttca ctaagaaatc gtggcttgca aaaaattcgt gaagaggcca
     1681 atttagaagg aggcatgtac gatctttcgt tagctgagaa cttcggtcca ctggacaccc
     1741 aggctgataa tgtgcgtaat ctggcgcgct tgtatgtgat tggatctggc ttctgggaag
     1801 tatatcctga gcaggctgta ttttatttca gccaggtggc tgcagcagtc cctggactcc
     1861 gcgacgcttc ggggtggtcc gcctcgggtc gcctttgggc tagcctggtt caatttggag
     1921 atttcctcgc ggagcgggat gattggtgtg cggcagagga gaaatataat gcagcctttg
     1981 ccatgcaaag caacacggag ctcatggaga aaatatccca ggcctatgac aaatgccacc
     2041 ctccgacaga gacgccaaaa ccgacgccga agccctcgga gactgccacg aacacaattg
     2101 tgcctcctgt gatcacggtt gaaacgccaa catctaccac ccttcctcct gcggtgacgc
     2161 aaacagcaac acgta
//
LOCUS       DBNHOH010000406         1607 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_984989, whole genome shotgun sequence.
ACCESSION   DBNHOH010000406 DBNHOH010000000
VERSION     DBNHOH010000406.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_984989"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="AC16V7_07150"
     CDS             complement(<1..571)
                     /locus_tag="AC16V7_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR6256932.1"
                     /translation="MSSTITSSQLIERDQSVVTPAIYRYTDGAYARGEGIYLIDFEGR
                     RYYDMVAGIATMSVGHCHPRVVAAITDQAQTLIHGAAHVGYMQPYVDILEKVLSLAPS
                     PLDQGKGVLLNSGSEAVEAAIKLARAVTGRSMVLAFMESFHGRPMGALALTASSSGYR
                     RHLTPLLSGVEHVPYPYCYRCPFGHKHRSR"
     gene            complement(634..1338)
                     /locus_tag="AC16V7_07155"
     CDS             complement(634..1338)
                     /locus_tag="AC16V7_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014531226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6256933.1"
                     /translation="MLAVQNLSVKYGAFHALRDLTLSIPEGQVVSIVGANGAGKTTLI
                     NTISGLIRPVSGAILFNGNDLTRMEGHAIASLGIAQVPEGRKLFPDMSVYENLLLGGY
                     SGRARSQRAESMEQVMELFPILRTRKNQLARTLSGGEQQMLAIGRAIMMRPRLMMFDE
                     PSLGLAPLVVRNIFEAITTLKRDGMTILLVEQNVRQSLQIADYAYVLETGHITLEGSG
                     QEVLDNDHTRKAYLGV"
     gene            complement(1340..>1607)
                     /locus_tag="AC16V7_07160"
     CDS             complement(1340..>1607)
                     /locus_tag="AC16V7_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein C-terminal
                     domain-containing protein"
                     /protein_id="HIR6256934.1"
                     /translation="LATEPSLLLLDEVAAGLNTVEVDDVLVLVRQLSQEGITLLIVEH
                     LMKVIMNLSHRIIVLDFGQKIAEGTPEEISRDPAVLEAYLGEEE"
BASE COUNT          370 a          439 c          427 g          371 t
ORIGIN      
        1 cgcgtgagcg gtgcttatgc ccaaatgggc agcgatagca gtatgggtat ggaacatgtt
       61 cgacgccgct cagcaatggg gtcaggtgac gccggtaacc gctgctgctg gcggttaacg
      121 ctagcgcccc cattgggcgt ccgtggaagc tttccataaa tgccagcacc atcgagcgac
      181 cagtgacagc ccgtgccagc ttgatcgccg cttcaacagc ctcactgccg ctgttgagca
      241 ggaccccctt tccttgatcg aggggagagg gagccaggct gagtactttt tccagaatat
      301 ccacgtaagg ctgcatataa cctacatgtg cggcgccatg gatcagcgtt tgcgcttgat
      361 ctgtgatcgc agctaccacg cgtgggtggc aatggcccac actcatggtg gcaatcccag
      421 ctaccatatc gtaataacgc cggccctcaa aatcgataag gtatataccc tcgccgcggg
      481 cataggcacc atccgtataa cgatagatgg ccggcgtgac gacgctttga tctcgctcga
      541 tcagttggct ggaagtaatt gtagatgaca tagttgatcc cttcttctag acagacttaa
      601 atgccatcaa tctaaacctg gctctgagag aacttacacg cccaggtaag ctttgcgagt
      661 atggtcgttg tccaggactt cttgcccgct cccttccaag gtgatatgtc cagtctccaa
      721 gacataagcg taatcagcga tttgcaagct ctggcgcaca ttttgctcga ctagcaatat
      781 ggtcataccg tctcgcttga gggtggtgat ggcttcgaag atgttgcgta caaccagggg
      841 ggcaagacct agcgacggtt catcgaacat catcaacctg gggcgcatca tgatggcgcg
      901 tccgatagct agcatctgtt gttctccacc tgagagcgtg cgcgcaagct ggtttttgcg
      961 ggtgcgcagg atggggaaca attccatgac ctgttccatg gattctgccc gttgtgagcg
     1021 agcgcgccca gaataaccgc cgagcaagag gttctcataa acgctcatat cggggaatag
     1081 cttgcgtccc tctggtactt gggcaatgcc taatgacgcg atcgcatgcc cctccattct
     1141 cgttaagtca ttaccattga aaaggatggc gcctgaaact gggcgaataa gcccggaaat
     1201 agtattgatc agggtcgttt tgcctgcccc atttgcacca acgatgctga ctacctgtcc
     1261 ttcaggaatg gagagggtga gatcgcgtaa ggcgtgaaac gccccatact tgacgctaag
     1321 attctgaacc gcgagcaatt tactcctcct ctccgagata cgcttcaagc acggcgggat
     1381 cgcggctgat ctcttccggt gtgccctcgg cgatcttctg gccgaaatcc agaacgataa
     1441 tgcggtgcga taggttcatg atcactttca tcaagtgctc aacgataagc aatgtgatgc
     1501 cctcctggct caattgacgc acaagcacca ggacgtcatc cacttcaacc gtgttgagtc
     1561 cagcagcaac ttcatccaac aacagcaagc tgggttcggt agccaag
//
LOCUS       DBNHOH010000407        12941 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1497152, whole genome shotgun sequence.
ACCESSION   DBNHOH010000407 DBNHOH010000000
VERSION     DBNHOH010000407.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12941)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12941)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12941
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1497152"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..202
                     /locus_tag="AC16V7_07165"
     CDS             20..202
                     /locus_tag="AC16V7_07165"
                     /inference="COORDINATES: protein motif:HMM:NF024930.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256935.1"
                     /translation="MSAIAAGGWHTCALTGSGGVKCWGSNWDCQLGNGYTHFLPHDVV
                     GFEGQYNMIYLPLVKK"
     gene            complement(352..1338)
                     /locus_tag="AC16V7_07170"
     CDS             complement(352..1338)
                     /locus_tag="AC16V7_07170"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase"
                     /protein_id="HIR6256936.1"
                     /translation="MFNIEDPEIRFALDAVRQASRLVQTVQSELVSPALVKDDRSPVT
                     VADYASQALVASLLSQAFPNDSLVGEEDSSELRHNQPVLEQVTGFVRRFMPEAGEEGI
                     CDWIDLGASAPSSRFWTLDPIDGTKGFLRSDQYAIALALVVEGEVQLGVLGCPKLSQA
                     YLPDYHGPGSLVLALRDQGTWTSPLEGEMQFKRLHASKQTDPVQARLLRSFESGHTNP
                     GQIDEFVQLLGVGAEPVRMDSQAKYAVLAAGQGELLVRLLSSSKPNYREKIWDQAAGS
                     LIIQEAGGTITDLDGKPLDFTAGRSLANNRGVLASNGYLHAAALGALRQVGA"
     gene            complement(1425..2807)
                     /gene="rimO"
                     /locus_tag="AC16V7_07175"
     CDS             complement(1425..2807)
                     /gene="rimO"
                     /locus_tag="AC16V7_07175"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HIR6256937.1"
                     /translation="MSSSKKSKTFHLVSLGCAKNTVDSQSMAQLLVGSGYRLQGDAAN
                     AEVVIVNTCGFIGPAKEESLQVLSDLAAHKRRGQLLIAAGCLTQRYGVEVARQVPVID
                     GILGTRRWMDIVDVVTRLRDGAHPEPIYHLPDAPTVGTDEGGALRAAVQGASAYVKIA
                     DGCRRPCAFCAIPLIKGTAVSRPVGAILNEARALQEMGVRELVLIAQDTTDYGHDLGL
                     KDGLAQLLEDLVRTVPHVDWIRVMYAYPGYVTDRLMEVMASQPQVLPYLDMPLQHAHP
                     AMLRRMRRPANMDWVHRTLEKMRASMPELALRTTFIVGYPGETEEEFQTLLDFVNEVR
                     FDRLGTFQFSFEPGTASEPLGDPIPPEVKEARYNRLMEAQQHISLEKNQMLVGKTLDV
                     LVEGQGRLEAEGNKHGEQTPISLGRSYRDAPEIDGMVLIEGEAPIGEIVPVRITGAMA
                     YDLAGMVDRE"
     gene            complement(2857..4110)
                     /locus_tag="AC16V7_07180"
     CDS             complement(2857..4110)
                     /locus_tag="AC16V7_07180"
                     /inference="COORDINATES: protein motif:HMM:NF024805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HIR6256938.1"
                     /translation="MSESIGQQLQQARQARSLTLEQVARATHLRVHYLQALEAGDLSR
                     VPSLVQARGFLRIYAEYLGLNVKTLLGELEGLASEPPVAPAPPEAQAEPVSADSAEPL
                     ASPAEATTAFTEIGLKLRQQRDVLGLSLDDVERHTHLRIFYLKALEAGELDRLPSPVQ
                     GRGMLSNYAEFLGMNPEPLLLRFADGLQAKLVERQAIKQPPRPRAERRPPRPPSLLRR
                     LFSAELLVGLSLVVVLVGFALWAAIRINALREEQQPAPTAPPIAAVLLATPVESPSPS
                     PVPPTATIPAPVAALAQPTEGTAQEALPPLASGEFVQVYVTVRQRTWMRVLVDGEVEF
                     EGRVIPGSAYQYSGEEKVEIVTGNGAALQVFYNQNDLGIIGLYGEVVHQVYTLQGIQS
                     PTPTMTMTPTTTARPSRTPRVTATP"
     gene            complement(4219..5193)
                     /gene="rplI"
                     /locus_tag="AC16V7_07185"
     CDS             complement(4219..5193)
                     /gene="rplI"
                     /locus_tag="AC16V7_07185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR6256939.1"
                     /translation="MKVLLLEDVYKLGRAGDVKRVADGYGRNYLIPQGLAVLATPGAL
                     KQVEHIRARAETSRAVLNQEMSGIAERLAKLTLAFPARAGETGKLYGSITSQMIADSI
                     QDKLGLEIDRRQLDAQPLRTLGEHNIHVRLTIDLVPEVRVIVYREGETVDLGEEEVAA
                     EAVAEAEVAEESAPEAEVAEVPAPEAEVAEVPEPEAEVAEVPEPEAEVAEVSEPEAEV
                     AEVPEPEAEVIEEAQPEAEVAEVPEPEAEVTEEAQPEAEVAEVPEPEAEVTEEAQPEA
                     EVAEVPEPEAEVAEESEPEAEVAEESEPEAEVAEEAQPDTEAPAKAAE"
     gene            5351..6148
                     /gene="recO"
                     /locus_tag="AC16V7_07190"
     CDS             5351..6148
                     /gene="recO"
                     /locus_tag="AC16V7_07190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIR6256940.1"
                     /translation="MPSRDRSFRVEAVVLRHSDWGEADRLLWLFTREMGKVKAVAKGV
                     RKLRSRKAGHLEPQTRVNLLLARGRDILLVTQAETVEAFLPLREDLKLWEYACHIIEL
                     LDRFTYEEDENRALYRLLVDTLGRLARQPQAELVVHYYEIRLLDLLGYRPHLFHCANC
                     SKEIQPVDQYFSADQGGVLCPSCGREVKGARPITMSALKYLRHFQRSSFSEAARARIP
                     HNVMREIEALMEYYLTYLLEQGLNSLAFIRRMRRGEMSNKARDQEII"
     gene            6246..7964
                     /gene="nuoF"
                     /locus_tag="AC16V7_07195"
     CDS             6246..7964
                     /gene="nuoF"
                     /locus_tag="AC16V7_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HIR6256941.1"
                     /translation="MSDFDLAPLQPVLAEFTTQGRTALLPALHAAQKLYGHLPEPVAA
                     EVGRALGVPLADVYGVIDFYSMFYSHPTGKTMVRVCTDPACALGGGEDVLERLCRQLN
                     VSPDEPSPDGAFQVERAPCLGLCEHAPAALVGDRAVGDAGSLQLDDLLSGHVSPLQAV
                     LGGDLRLLTANCDNGQPTSLQEYAARGGFAGLKKALRMPPDSVIAEVKASGLVGRGGA
                     AFPTGIKWEGAAKAEGSPKYVICNADESEPGTFKDRLLLEGDPQLILEGMLIAAYATG
                     ASRGYIYIRGEYPHAYQIMEDALSQARQAGYLGENILGTGFEFDLELRLGAGAYICGE
                     ETALFESIEGKRGFPRVKPPFPTTHGVFGKPTVINNVETLCNVPTILQHGAAAYRQMG
                     TEKSPGPKLFCVSGDVARPGLYEVPFGITIRRLVYDLAGGVRDGRSLQTILFGGAAGA
                     FATPDQLDVRMSFEDLRAAGLPLGSGVVMVFDERRDLRDVLVRLGRFFAHESCGKCYP
                     CQLGTQRQYEILSRLAAGETRPGDAERLSDVGWTMTDASLCGLGQTAATAVLSAMKIW
                     PELFGN"
     gene            8055..8774
                     /locus_tag="AC16V7_07200"
     CDS             8055..8774
                     /locus_tag="AC16V7_07200"
                     /inference="COORDINATES: protein motif:HMM:NF024900.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR6256942.1"
                     /translation="MPEKVTLTIDGSSVTVPAGTTILKAAEQIGEKIPTICYHDHCTA
                     NALCRLCVVEVEGARVLSPSCVAQVSEGMKVETRSPKVERSRRTILEMLASAVDLSEA
                     PEILDFMGEYDVHDEPFPGSERRQPPLLDDNPMYMRDYAKCVLCWRCVQVCADDAQYT
                     YAINFSGRGFETSIGTFFDKTMPDTTCVFCGQCVGVCPTGALKPKREWLLEQGMTPDE
                     VMQMTRTERRGKRDSRVENQE"
     gene            8847..11009
                     /gene="fdhF"
                     /locus_tag="AC16V7_07205"
     CDS             8847..11009
                     /gene="fdhF"
                     /locus_tag="AC16V7_07205"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HIR6256943.1"
                     /translation="MIKPDRVVSTTCPYCGVGCNLELHVKDETIYRVTSPFDAVVNQG
                     NLCVKGRFGYDFVYHRRRVTTPLLRKVRQEPGKRTQAFERDDWRQVSWDEALDYVADR
                     LVEIYRRDGPDALAVYCCAKATNEDNYLLQKMFRSLFRTNNVDHCTRLCHAGSVVALQ
                     QAVGSSAMSNTASEVIKSDVFIVTGSNTPENHPIIALQMKAAVMKHGAKLIVIDPRRS
                     ELCDFATLWLPLKPGTNVPVFSAMAHVIIRDGLVNRQFVEQRTEGFDEFARSLEKFTP
                     EYAEMVSGVKRDLIVQAAHMYASAERGAIYWGMGISQLSHGTASALAVIHLALLTGHI
                     GREGTGLNPLRGQNNVQGASDMGSMPYHYPGYMLVDNPDNAAKWERAWNIEPGGLSLK
                     RGLTTTEILSHARPGGVRSLYIMGENPMMSEPNLNLTRHHMEQLEFLVAQDLFINESG
                     AFADVILPSTSWAEKDGTFTNTDRRVQRVRQAIAPRGDSRPDWQIICDLAKRIEARLG
                     RPNSAGWDYAQPSEVLAEVGRVVPEFTGVTYERLEESGLQTPVWDESHPGTPYLFSQS
                     FPRGRGKFHPLEFVPSVELPDDEYPFILSTGRVLEHWHGGTLTRHSKLDDLYPEARVE
                     LHPTDAALIDIRDGQAVRVSSRRGTVALRATVTEKTRAGVVFIPFHFAEAAANLLTID
                     ALDPQAKIPEFKACAVKIEPVSDADLPHPEAVLRRGRY"
     gene            11181..11645
                     /locus_tag="AC16V7_07210"
     CDS             11181..11645
                     /locus_tag="AC16V7_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012036844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HIR6256944.1"
                     /translation="MSHPCEITEQPAQPTLSIRTRAAVQNLPQVLGDGYGAIMQYLGE
                     LGISPTGHPFVIYYNMDMQDLDIELGFPVSQPLPGKGQIHAARMEGGKVAEYLYVGPY
                     ADCGPAYEELAQFVKDQGYEATGVAIEYYLNDPSQPPYEEPQTRIVLPLKNT"
     gene            11874..12662
                     /locus_tag="AC16V7_07215"
     CDS             11874..12662
                     /locus_tag="AC16V7_07215"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017601844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HIR6256945.1"
                     /translation="MSCKNYLVDMDGVLVRGTSTIPGAAQFIDRLVTCGCKYLILTNN
                     PLYTPGDLAHRLQVAGLDVPETHIFTSALATAQFLDRQKPKGKAFVIGESGLTSAIHS
                     VGYIITDLEPDYVILGETFAYNLELVTKAIRLISAGAKFIATNPDPSGPSESGLVPAC
                     GAMAALIEKASGVSPFFVGKPNPLMMRMALNYLDVHSENTVMVGDRMDTDIITGVMSG
                     METILVLSGVTKQGEIDRYPYQPTYIYDSVKDIEPCKPKKRVKE"
     gene            12666..12920
                     /locus_tag="AC16V7_07220"
     CDS             12666..12920
                     /locus_tag="AC16V7_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017291331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HIR6256946.1"
                     /translation="MSEEKVRTEEFEVSGDELVAKIKELVHEGNIRRISIRNEEGRTL
                     IDLPLTISVVGAILVPQLAALGAIAALVTHCTIVIEKVDE"
BASE COUNT         2715 a         3845 c         3781 g         2600 t
ORIGIN      
        1 gagcgggctg tccagcgggg tgagtgcaat tgccgctggg ggatggcata cttgcgcact
       61 gactggctcc ggcggggtca agtgctgggg tagtaactgg gattgtcagc tcggaaacgg
      121 ctacacacac tttttgcccc acgatgtggt cgggtttgaa gggcaataca atatgatcta
      181 tctacccctt gtaaaaaagt gagcaagcaa ccattgccct gaagggtttc ccagggacat
      241 cgcgttttcg aaagctgtag aaggtctgat tggaggaaaa gccctgcccg cctggaggcg
      301 ggcagggcag cggtatgtcc aggggagcca ttacggttga gaacaggctc gtcaagcccc
      361 aacctgccgc aacgccccca gcgccgccgc gtgtagatac ccattggagg ccagcacacc
      421 gcggttgttc gccaggctgc gcccagcagt aaagtccagg ggcttcccgt ccaggtcggt
      481 gatggtcccg ccagcttcct ggatgatcag cgaaccggca gcctggtccc atatcttctc
      541 gcggtagttg ggtttggatg acgagagcaa gcgcaccagc agctcaccct gaccggcggc
      601 caggacagca tacttggcct ggctatccat gcgcaccggt tcggccccca cgcccagcaa
      661 ctggacaaat tcatcgatct gaccggggtt ggtatgacca gactcaaagg agcgcagcaa
      721 gcgtgcctgg actgggtccg tctgcttgga ggcgtgcagg cgcttaaatt gcatctcgcc
      781 ctccagcggg gatgtccagg taccctggtc ccgcagagca agcaccagcg agcccggtcc
      841 gtgatagtct ggcaggtacg cctggctcaa tttcgggcag cccaacacgc ccaattgcac
      901 ctcaccttcc acgaccagcg ccagggcaat agcgtattgg tcgcttcgta ggaaaccctt
      961 agtgccgtcg atggggtcca gcgtccaaaa gcgcgaagac ggtgctgacg ccccaaggtc
     1021 gatccaatca catatgccct cctcacccgc ttcaggcatg aaacggcgca cgaaaccggt
     1081 cacctgctcc aggacaggct gattgtggcg caactcgctg gagtcctcct cgcccaccag
     1141 ggagtcgttt gggaaggcct ggctgagcag gctcgctacc aaagcctggg aggcgtaatc
     1201 cgccaccgtt accggcgagc gatcatcctt gaccagggca ggggaaacca gctcggactg
     1261 gacggtttgt accagacggg acgcctggcg caccgcgtcc aaggcaaaac ggatctcagg
     1321 gtcttcaata ttgaacatat attttcccag tcaacaaaat cgaacaggat gtacaggata
     1381 aataggatca tattttcatc ctgtttatcc tgttaattct ttctctattc cctatcgacc
     1441 atcccagcca ggtcgtaggc catggccccg gtgattcgca ccggcacgat ctcgccgatg
     1501 ggagcttcgc cttcgatgag caccatgcca tcgatctccg gggcgtcgcg gtagctgcgc
     1561 cccagcgaga tgggggtctg ctccccatgt ttatttccct ccgcctccag gcggccttgc
     1621 ccctccacca gtacgtccag ggtcttgccc accagcatct ggtttttctc caatgagatg
     1681 tgctgctggg cttccatcag gcgattatag cgcgcctcct tgacctcggg cgggatgggg
     1741 tcaccgagcg gctcgctggc cgtgcctggc tcgaaggaaa actggaacgt gcccaggcgg
     1801 tcgaagcgca cttcattcac aaagtccaac agcgtctgaa actcttcttc ggtctcacca
     1861 gggtagccca caatgaaggt ggtacgcaag gccagctcgg gcatagaggc gcgcattttt
     1921 tccagggtgc gatgcaccca gtccatgttt gcggggcggc gcatgcgccg cagcatcgcc
     1981 gggtgagcgt gctgcagagg catatccagg taggggagca cctgcggctg gctggccatg
     2041 acttccatca ggcggtcggt gacgtaaccg gggtaggcat acatgacgcg aatccagtcc
     2101 acatgcggga cggtgcgcac caggtcttcc agcaattgcg ccaggccatc tttcagcccc
     2161 aagtcgtgcc cgtaatccgt tgtatcctgg gcaatcagga ccagctcgcg aacacccatc
     2221 tcctgcaaag cgcgggcttc attcaggatg gcacccaccg ggcggctgac cgcggtgccc
     2281 ttgatcagcg ggatggcgca gaaggcgcaa ggtcgccggc aaccgtcagc gatcttgaca
     2341 taggcgctgg caccctgcac cgcagcgcgc agcgcgccac cctcatcggt tcccaccgtg
     2401 ggtgcatctg gcagatggta aatgggctcg ggatgggcac cgtctcgcaa gcgggtcacc
     2461 acatccacga tatccatcca gcgacgggtc cccaggatgc cgtcaatgac aggcacttgc
     2521 cgggcaacct cgacgccata acgttgcgtc aggcatccgg ctgcgataag cagctggccg
     2581 cggcgcttgt gcgcggccag atcgctcaaa acctgcagcg attcctcttt cgccgggccg
     2641 ataaaaccgc aggtatttac aatcaccact tctgcattgg cggcatcacc ctgcaggcgg
     2701 taacctgatc caaccagtaa ttgcgccatc gactgcgaat caacggtgtt tttcgcacac
     2761 cctaacgata ccaggtgaaa tgttttcgat ttcttactag agctcatcaa taagttgatt
     2821 ccttgttctt aatatatctt gaaagcatac tcacgctcat ggagtagctg tgactctggg
     2881 ggtgcggctg ggacgtgccg tggtggtggg cgtcatcgtc atggtaggtg ttggggattg
     2941 tatgccctgc agggtgtaga cctggtgaac cacctcgcca tacaggccaa tgatcccgag
     3001 gtcattttga ttataaaaca cctgtaacgc agctccattg cctgtcacta tctcaacctt
     3061 ttcctcgcca gaatactggt aggcgctgcc cggaataaca cgcccttcaa actccacctc
     3121 tccatccacc agcacgcgca tccaggtgcg ctggcgtaca gtgacataga cctggacaaa
     3181 ctccccgctg gcaaggggtg gcagggcctc ctgggcggtg ccttcggttg gctgtgccaa
     3241 ggcagccaca ggtgccggga tagttgccgt gggcggcaca ggcgatggtg aaggggattc
     3301 aacaggcgtg gccagcagga cggctgcaat ggggggagca gtaggagcag gctgctgctc
     3361 ttcccgcagg gcgttaatgc gaatggcagc ccacagggca aatccgacta gcactaccac
     3421 cagggatagg ccaaccagca actcggcgga aaataagcgc cgcagcaagg acggtgggcg
     3481 gggagggcga cgttcggcgc gagggcgcgg gggctgcttt atcgcctgtc gctcaaccag
     3541 cttagcctgc aagccatcag caaagcgcaa aagcagaggc tcaggattca tccccagaaa
     3601 ttcagcatag ttgctgagca tgccgcgtcc ctgcaccggc gagggcaagc gatctaattc
     3661 acccgcctcg agcgccttta gataaaagat acgcaggtgg gtgtgtcgct cgacgtcgtc
     3721 taaagacagg cccagcacat cgcgctgctg gcgcagcttc aggccaatct cagtgaaggc
     3781 tgtggttgcc tcagccgggc tggcgagtgg ctcggcagaa tcagctgaga caggctcagc
     3841 ctgagcttca ggtggagcag gggcaactgg gggctcagag gccagcccct ccagctcgcc
     3901 cagcaatgtc ttaacattca acccaaggta ttcggcataa atacgcagga aaccgcgcgc
     3961 ctgcaccaaa gagggcactc tggagagatc accggcttcc aatgcttgca agtaatggac
     4021 gcgcaggtgc gtcgctctgg cgacttgctc caaggtgagc gatcgcgcct ggcgtgcttg
     4081 ctggagctgt tgaccgatcg actctgacat tacggttacc aggtatgatt acggcaacag
     4141 gggcggggtt tccagacgct ggttggttac cccgcccctg gatgctacac aggagcgctg
     4201 tttcctgccc ggacggaact actcagcagc ttttgcaggc gcctctgtat caggctgagc
     4261 ttcctcagcc acttcagctt ctggctctga ctcttcagcc acttcagctt ctggctctga
     4321 ctcttcagcc acttccgcct ctggctctgg cacttcagcc acttctgcct ctggctgagc
     4381 ttcttcggtc acttccgctt ctggctctgg cacttcagcc acttcagcct ctggctgagc
     4441 ttcttcggtc acttctgcct ctggctctgg cacttcagcc acttctgcct ctggctgagc
     4501 ttcttcgatc acttccgctt ctggctctgg cacttcagcc acttctgcct ctggctctga
     4561 aacttcggca acttctgcct ccggctctgg cacttcagcc acttctgctt ctggctctgg
     4621 cacttcagcc acttccgctt ctggcgctgg cacttcagcc acttctgctt ctggcgctga
     4681 ctcttcagct acttcagcct cagccactgc ttcagcggca acttcctcct cgcccagatc
     4741 gaccgtctct ccctcacggt agacgatcac ccgcacttcg gggaccagat cgatagtgag
     4801 gcgcacatga atattatgtt cgcccagcgt gcgcaggggc tgtgcgtcca gctggcggcg
     4861 atcgatttcc aggcccagct tatcctgaat agagtcggca atcatctggg aagtgatgga
     4921 accgtacaat tttccggtct ctccagccct agccggaaag gcaagggtga gcttagctaa
     4981 ccgttcggcg atcccgctca tctcctggtt gaggactgcg cggctggtct ctgcgcgggc
     5041 gcggatgtgc tccacctgtt tcagagcccc gggggtagcc agcactgcca gaccttgcgg
     5101 gatcaggtag ttgcgaccat agccatcggc gactcttttg acatcgccag cgcgaccaag
     5161 tttatacacg tcttccaata acaaaacttt catttctttc tcaagccctt tcaatagaat
     5221 gtctcgacat acaaacccgg gcgaagggta caacgctccg ggcaggggga ttttaccaga
     5281 tattttaaga aattgggcac aaaccgctct ccatcccgca aacgcccgat ttcgccatta
     5341 taatcaagcc atgccttcca gagaccgctc cttccgtgtc gaggcggtgg tgctgcgcca
     5401 cagtgactgg ggtgaggctg accgcctgct gtggctgttc acgcgcgaga tgggcaaggt
     5461 gaaagcggtg gcaaagggcg tccgcaagct gcgttctcgc aaggcaggtc acctcgagcc
     5521 gcaaacgcgc gtcaacctgc tgctggcacg cgggcgcgat atcctgcttg tcacccaggc
     5581 agagacagtg gaagccttcc tgcccttgcg ggaagatctt aagctgtggg agtatgcctg
     5641 ccacattatc gagctgcttg accgtttcac gtatgaagaa gacgagaatc gggctctata
     5701 ccgcttgctt gtggacacgc tagggcggtt agccagacag ccgcaagcgg aattggtggt
     5761 gcactactac gagatacgcc tcttggattt actgggctac cgcccgcatc tatttcactg
     5821 cgcaaactgc agcaaagaga tccagccagt agaccagtat ttctccgcag atcagggggg
     5881 tgtgttgtgc ccatcttgcg ggcgagaggt gaaaggtgcg cgtcccatca ctatgtcggc
     5941 gttgaaatac ctgcgccact tccagcgcag cagttttagc gaggccgctc gtgcccgcat
     6001 cccccacaac gtgatgcgcg agatcgaggc gttgatggaa tattatttga cttatttgtt
     6061 agagcagggg ttaaattcac tggcgttcat tcgcaggatg agaaggggag agatgagcaa
     6121 caaagcgaga gatcaagaaa ttatatgaac tggtcatccc cttcgctcaa ctcactagct
     6181 aactgtccaa taccgttgaa aattatcatc aagatgggta caatggacga tgatatggag
     6241 gaattatgtc ggattttgat ctggcacccc tgcagcctgt attagctgaa ttcactaccc
     6301 aggggcgcac tgccctatta cccgccctgc atgccgccca gaaattgtac gggcacctgc
     6361 ccgagccggt cgccgccgag gtaggccgcg ccctgggtgt gcccttagcc gatgtgtacg
     6421 gcgtgatcga cttttacagc atgttctaca gccatcctac cggcaagacg atggtgcgcg
     6481 tgtgcaccga tccagcctgt gccctggggg gtggcgagga tgtgctggag cgcctgtgtc
     6541 gccagctcaa cgtcagtcca gacgaaccca gccccgacgg ggcattccag gtggagcgcg
     6601 ctccttgcct ggggctttgc gagcacgcgc cagcagccct ggtgggcgat cgggcggtcg
     6661 gggacgccgg ctcgctgcag ctcgacgacc tgctgtcggg tcatgtttca ccactccaag
     6721 ccgtgctggg tggtgacctg cgcctcctga ccgccaactg cgataacggg cagccgacat
     6781 ccttgcaaga gtacgccgcc cgagggggat tcgccgggct gaagaaagct ttgcgcatgc
     6841 cccccgacag cgtgatcgcc gaagtaaagg cctccgggtt ggtggggcgc ggcggggcag
     6901 ccttccccac cgggattaag tgggagggcg ccgccaaggc cgaaggctcg ccaaagtatg
     6961 tgatttgcaa cgccgatgaa tctgagcctg ggacgttcaa agatcgcctc ctgctggaag
     7021 gtgacccgca gctcatcctc gaaggcatgc tcatcgctgc ttacgccact ggagcaagcc
     7081 ggggatatat ctacatccgt ggcgagtacc cccatgcata ccagatcatg gaagacgccc
     7141 tttcccaggc gcgccaggcg ggctacctgg gggaaaatat cctgggaacg ggcttcgagt
     7201 tcgacctcga gctgcgcctt ggcgcggggg cttatatctg cggtgaggag accgccctgt
     7261 tcgagtctat cgagggcaag cgcggtttcc cgcgcgtcaa gccgcccttc cccaccaccc
     7321 acggggtgtt tggaaaaccc accgtcatta acaatgtaga gacgctgtgt aacgtgccaa
     7381 ccatccttca gcacggggca gcagcctacc gccagatggg tacggagaaa tctccaggac
     7441 ctaagctgtt ctgtgtctca ggcgacgtgg ctcgtcccgg gctgtacgag gtgccattcg
     7501 gcatcaccat ccgccgcctg gtgtacgacc tggcaggagg cgtacgcgac gggcgatcgc
     7561 tgcagaccat cctgtttggt ggcgccgcag gggccttcgc cacgcctgac caattagacg
     7621 ttcgcatgag cttcgaagat ttgcgcgccg cgggcttgcc gctaggttct ggtgtagtga
     7681 tggtcttcga cgagcggcgc gacctgcgcg atgtgctggt acggttggga cgattcttcg
     7741 cccacgaatc gtgtggcaag tgttacccct gccagcttgg cacgcaacgg cagtacgaga
     7801 tcctctcccg gctggcagca ggtgagacgc ggcctggcga tgccgagcgc ctgtctgatg
     7861 tgggctggac catgacggac gcttctctgt gcggcttggg acaaactgct gccacggcgg
     7921 tgctgagtgc catgaagatc tggcctgaac tatttggaaa ttaagaagag atgccgcgct
     7981 gcgcgaatta cacaaatgct tagcgatatt cgcgtaattc gcggatatta cccatattat
     8041 tcgaaggagc tcgcatgcca gagaaagtaa cgcttaccat cgacggctct tcggtaactg
     8101 tcccggcagg gaccacgatc ctcaaagccg ccgagcaaat cggcgaaaag atacccacga
     8161 tctgctatca cgatcattgc acggcaaacg ccctgtgccg cttgtgcgtg gtagaggtag
     8221 aaggcgcgcg cgtgctcagc ccttcctgcg tagcacaggt aagcgagggc atgaaagtag
     8281 agacgcgcag ccccaaggtg gagcgcagcc ggcgcaccat cctggagatg ctggcctcgg
     8341 cggtggacct gtcagaggcg ccggagatcc tggacttcat gggagagtat gacgttcatg
     8401 acgagccctt cccagggagc gagcgacgcc agccacccct gttggacgat aatcccatgt
     8461 acatgcgtga ctacgccaag tgcgtgttgt gctggcgctg tgtacaggta tgcgccgatg
     8521 acgcccagta cacctacgcg atcaacttca gcgggcgtgg cttcgagacc tccatcggca
     8581 catttttcga taaaaccatg cctgatacca cctgtgtgtt ctgcggacag tgcgtgggcg
     8641 tgtgccccac tggtgcattg aagcccaagc gtgaatggct gctcgagcag gggatgaccc
     8701 cagatgaggt catgcagatg acgcgaaccg aacgcagggg gaagcgagat tcgagagtag
     8761 agaatcaaga gtagcgaatt gagaaacgca gattacgaat ccccaatctc gatgctcgaa
     8821 tcccgattct ctttttggag gcaacaatga tcaagcctga ccgagtagta tcgaccacct
     8881 gcccttattg cggggttgga tgtaacctgg aattgcacgt caaggacgaa acaatttatc
     8941 gggtgacctc accctttgac gcagtagtga accaggggaa cctgtgcgtg aaagggcgct
     9001 ttggctacga ttttgtctat caccgccggc gagtgacaac cccactgctc cgcaaggtgc
     9061 gccaggagcc tggaaagcgc acgcaagcct tcgagcggga tgattggcgc caggtctcct
     9121 gggatgaagc gctggattac gtagctgacc ggctggtaga aatctatcgc cgcgatgggc
     9181 cagacgccct ggcagtatat tgctgcgcca aggccaccaa cgaggataac tacctgctac
     9241 agaagatgtt tcgctccctg ttccgcacca ataacgtaga ccactgcacg cgcctgtgcc
     9301 atgctggctc ggtggtggcg ctgcagcagg cagtcggatc gtcagccatg agcaacaccg
     9361 cctccgaggt gatcaagagc gatgtattca tcgtcaccgg ctcgaacaca cccgagaacc
     9421 accccatcat cgcactgcag atgaaagcag ccgtgatgaa gcacggcgcc aagctgatcg
     9481 tgatcgaccc gcggcgctca gagctgtgcg actttgccac cctgtggctg ccgctcaagc
     9541 caggcacgaa cgtgcccgta tttagcgcca tggcgcacgt cattatccga gatggcctgg
     9601 tcaaccgcca gttcgtggag cagcgcaccg agggcttcga cgagttcgcc cgctccttgg
     9661 agaagttcac cccagagtat gccgagatgg tttcaggggt aaagcgcgac ctgatcgtgc
     9721 aagcagcgca catgtacgcc agcgccgagc gaggggctat ttactggggc atgggcattt
     9781 cgcagctctc gcatggcact gccagcgccc tggcagtcat ccacctggcg ctgctcaccg
     9841 ggcacatcgg gcgagagggc accgggctga atcccttacg gggacagaac aacgtgcagg
     9901 gcgcgtcaga catgggctcc atgccttacc attacccggg ctacatgctg gtagataatc
     9961 ccgacaatgc ggccaaatgg gagcgggcct ggaatatcga acccggcggg ttgagcctga
    10021 aacgcgggct gaccactacc gaaatcctct cccacgctcg ccctggcgga gtgcgatcgc
    10081 tctacattat gggtgaaaac ccgatgatga gcgagcccaa cctgaacctg acccgccacc
    10141 acatggaaca gctcgagttc ctcgtggcgc aggacctgtt tatcaacgaa tccggagcgt
    10201 ttgccgatgt gatcctgcca tctacttcct gggcagaaaa agacggcacg ttcaccaaca
    10261 ctgaccggcg ggtgcagcgc gtgcgccagg caatcgcccc acgcggagat tcgcgccccg
    10321 actggcagat catctgcgac ctggccaagc gcatcgaggc tcgcctgggg cgcccaaact
    10381 ccgcaggctg ggattatgcc cagccttccg aggtgctggc cgaggtgggc cgcgtggtgc
    10441 cggaatttac cggcgtgacc tacgagcgct tagaagagag tggcttgcag acaccggtat
    10501 gggatgaaag ccatccgggc acaccttacc tgttcagcca gagctttcca cgcgggcggg
    10561 gcaagttcca cccgctggag tttgtgccca gcgtggagct acctgacgac gagtacccct
    10621 tcatcctgag caccgggcgg gtgctggagc actggcacgg cgggacgctc acccgccatt
    10681 ctaagctgga cgatctctac ccagaggcgc gggtggaatt gcaccctacc gatgcagccc
    10741 tgatcgatat tcgcgacggt caagcggtgc gcgtctcttc gcggcgcggg acggtggcgc
    10801 tgcgcgccac ggtgaccgag aagacccgcg ccggggtggt ctttatcccc ttccactttg
    10861 ccgaggctgc cgccaacctg ctgaccattg acgccctcga cccgcaggcc aagatcccgg
    10921 aattcaaagc ctgcgcggtg aagatcgagc cggtcagcga cgcggacctg ccgcacccgg
    10981 aggctgtgct acggcggggg aggtattaag gcaacgcgcc aaatcatcag gcgtacggat
    11041 cgagattaat agaggttttg tagccatcat tccagtgccg tggggacggc agccgcacca
    11101 atcttgacaa aatagaacgc atgtgctatt attcacaaaa gattttcgtt caatctttac
    11161 atctattggg agggagtgca atgtctcacc cgtgcgaaat cacggagcag cctgctcagc
    11221 caacactgag catccgcacc agggctgcgg tgcagaatct accacaggtg ctgggagatg
    11281 gctatggagc tatcatgcaa tacctgggcg agctggggat aagcccaaca ggacatccat
    11341 tcgtaatcta ttacaacatg gacatgcaag atttagacat cgagctgggc ttccccgtgt
    11401 cccagccgct gccgggcaag ggtcagatcc acgctgcccg catggagggg ggtaaagtcg
    11461 cggagtacct gtatgtcggt ccctacgcag attgcgggcc cgcttacgag gaattggccc
    11521 aattcgtcaa agatcagggt tatgaagcca ccggcgtggc catcgagtat tacctcaacg
    11581 accccagcca accaccctac gaagagccac aaacccgcat tgttttgccg ctgaaaaaca
    11641 cctgatcaag ttatttacac cccaaagggc acaaaagtag caaacataga acaccccaat
    11701 ttaataattg ttggattttg tagttcgtat gcccaccccc tcgttccccc tcccaccgcc
    11761 tgcggcttcg ggagggggga tgattgacgc atatcacagt attccttata atgagtttgg
    11821 gcggtgaaat caccaacaat ttatctccgc tggatggcac tggagcatgg ataatgtcct
    11881 gtaaaaatta cctggtcgat atggatgggg tgctggtgcg cggcaccagc actatccctg
    11941 gcgcagccca gtttatcgat cgcctggtga cctgcggctg caaatacctg atcctgacca
    12001 ataacccgct ttacacgccg ggcgacctgg ctcaccgcct ccaggtagcc gggctggatg
    12061 tcccagagac gcatatcttc acctctgcgc tggcaactgc ccaattcctg gatcgccaga
    12121 aacctaaggg caaagccttt gtgatcggag agagcggtct gacttccgcc atccacagtg
    12181 tgggctacat cattacggac ctcgagccgg attacgtcat cctgggcgag acctttgctt
    12241 acaacctgga gcttgtcacc aaagcgatcc gattaatctc ggcaggggcg aagttcatcg
    12301 ccaccaatcc agacccatcc gggccttccg agagcgggct ggtccccgct tgcggggcga
    12361 tggcagccct gatcgagaaa gccagcgggg tctcaccgtt ctttgttggc aagccgaacc
    12421 cgttgatgat gcgcatggcg ctcaattatc tggatgtcca ttccgagaac accgtgatgg
    12481 tcggcgaccg catggacacg gatatcatta caggtgtcat gtctggaatg gaaaccattt
    12541 tggtgctcag cggggtaaca aaacaaggcg agatagaccg ttatccatac cagccgacct
    12601 atatctatga ttctgtgaag gatatcgaac cgtgcaaacc gaagaaacgc gtaaaggagt
    12661 gaagcatgtc ggaagagaaa gtccgaaccg aagaatttga ggtcagtggc gacgaactgg
    12721 tagccaagat caaggagctg gtgcacgagg gcaatatccg gcgcatcagc atccgcaacg
    12781 aggagggccg caccctgatc gacctgccgc tcaccatcag cgtggtgggg gcgatcctgg
    12841 tgccgcaact ggcggcgctc ggcgcgattg ccgcactggt aacgcattgc acgatcgtta
    12901 tcgagaaggt ggacgagtag agccggaatc atcctcccgc a
//
LOCUS       DBNHOH010000408         1756 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_472047, whole genome shotgun sequence.
ACCESSION   DBNHOH010000408 DBNHOH010000000
VERSION     DBNHOH010000408.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_472047"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..964
                     /locus_tag="AC16V7_07225"
     CDS             167..964
                     /locus_tag="AC16V7_07225"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6256947.1"
                     /translation="MRVRLKDVYLAVEERGDGRPLLFIHGYPLRGAMWAPQIEGLADV
                     ARVIVPDLRGHGESDATTGAYSMDLLAEDCREMLDAMGIIEPIVICGLSMGGYVTFAF
                     YRKYPERVAGLILAATRAVDDSPQGKANRDKAADLARQGGTGAIVESMLPKMFAPKTY
                     LERADLVQNLREIMQGVSLDGILGDLMGMKSRPDSTPTLGQIDKPTLILHGSDDQLIP
                     VSDASSMQSVVAGSVLRVLQDSGHLLNLEQPDLFNQAIREYLKELHR"
     gene            961..>1756
                     /locus_tag="AC16V7_07230"
     CDS             961..>1756
                     /locus_tag="AC16V7_07230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR6256948.1"
                     /translation="MKFGVIYPQTEYGSDPSAIKDFAQTVEDLGYAHIGCFDHVLGVN
                     PDRPGGWQGQYTYQSAFQEPFTLFSFFAAVTQRTLFTTGVLILPQRQTALVAKQAATL
                     DVLSEGRLRLGVGLGWNAVEYTCLNENFHNRGRRLEEQIEVLRLLWTEPLASFSGRWH
                     TIPDAGINPLPVQRPIPIWFGGRAEPALKRAARLGDGWVAPYRTPTEAMPNFEKFAQY
                     LDEADRSWEKYSVEVRLYYGPGDPDIWKKLVREWQVVGVDNISFNTM"
BASE COUNT          439 a          385 c          486 g          446 t
ORIGIN      
        1 ccgccggata tcgtccaaaa agaacgagat aaactggcgg ttttagatga aactgtgcag
       61 aaactcaggg agcagttaaa atcaatctcg tgaagatcgc ccattatcgg gtcggcatag
      121 gtgatagaaa aattcattgt ccttgaagta tttattgagg tgagctatgc gagttcgtct
      181 taaggatgtt tatcttgctg ttgaggagag gggtgatggt cgcccgttgc tgtttatcca
      241 cggttatccc ttgaggggtg ccatgtgggc gccacaaatc gaaggcttgg cagatgtggc
      301 gcgggtaatt gttccagacc tgcgcggtca tggcgaatct gatgccacaa caggtgcgta
      361 ctcaatggat ttgttggcag aagactgtcg agaaatgtta gatgcaatgg gcattattga
      421 gcctatcgtg atctgtgggt tatcgatggg aggctatgtg acctttgcct tctatcgcaa
      481 atatccggag cgtgtggcgg gcttgatcct ggcagctact cgggctgtag atgactcacc
      541 acagggcaaa gccaatcgcg ataaagccgc tgatctcgct cgccagggtg gtacgggcgc
      601 aatcgtggaa agcatgttac ccaaaatgtt tgcaccgaaa acatacctcg agcgtgcgga
      661 tttagtacaa aatttgcggg agatcatgca aggtgtgtct ctcgatggaa tactaggcga
      721 tcttatgggg atgaagtctc gacctgattc gacccccacc ttgggtcaaa ttgataaacc
      781 aaccctgatc ttgcacggca gtgacgatca acttattcca gtgtctgatg caagttcgat
      841 gcaatcagtt gtcgcaggat cagttctgcg cgtgttgcag gactctggtc acttgttgaa
      901 tctcgaacaa cccgatttgt tcaaccaagc catacgcgaa tatcttaagg agctccatag
      961 atgaaatttg gtgtgattta tccgcagacg gaatatggtt cagatcctag cgcaatcaaa
     1021 gattttgctc agaccgttga agatttgggt tatgctcata tcggttgttt tgatcatgtg
     1081 ttaggtgtca atccagaccg ccctggtgga tggcagggac aatacacata ccaatcagct
     1141 tttcaagaac cattcacact gttcagtttt tttgctgcag tgacacaacg taccctcttt
     1201 acaacgggag ttttgatact gccacagcga cagacggcac tggtggccaa gcaggctgcc
     1261 actctggatg tgttgagcga agggcgcctg cgactgggtg tgggtctggg ttggaacgca
     1321 gttgagtaca catgcttaaa tgagaatttt cataaccgag ggcgccgctt ggaagaacaa
     1381 atagaggtcc tgcggttgtt atggactgag ccgttagcta gcttctctgg gcgatggcac
     1441 acgatccctg atgcaggtat caacccgcta ccggttcagc gaccaatccc gatatggttt
     1501 gggggacgcg ccgaaccagc cttgaaacgt gctgcccgac taggcgatgg ctgggtagcc
     1561 ccataccgaa cacctaccga agcgatgcct aattttgaaa aatttgccca atacctcgat
     1621 gaggcggatc gaagctggga aaaatatagt gtcgaggtaa ggctgtatta tggaccggga
     1681 gatccggata tctggaaaaa acttgtccgt gaatggcaag tggttggggt tgacaacatt
     1741 tcattcaaca caatgg
//
LOCUS       DBNHOH010000409         7937 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1625192, whole genome shotgun sequence.
ACCESSION   DBNHOH010000409 DBNHOH010000000
VERSION     DBNHOH010000409.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7937)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7937)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7937
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1625192"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..2445
                     /locus_tag="AC16V7_07235"
     CDS             178..2445
                     /locus_tag="AC16V7_07235"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HIR6256949.1"
                     /translation="MTKITREDALEYHRLGGKPGKVSVVPTKPMATQRDLSLAYTPGV
                     AVPVLEIEKDPELGYEYTSKGNLVAVVSNGTAILGLGDRGALASKPVMEGKGVLFKRF
                     ADVDVFDIEVDTHDPDEVIQVVAAISPTFGGINLEDIKAPECFYIEEKLKEMLNIPVF
                     HDDQHGTAIISGAALMNGLEIVGKRIEEARITISGAGASAISCAELMVRLGARRDNIL
                     LVDTRGVVYQGRTEGMNKYKERFAHDTDARTLADAMRGADVFYGLSIANILTPEMVKT
                     MAESPLIFAMANPDPEIRYELAKEARPDAIVATGRSDYPNQINNVLGFPFIFRGALDV
                     RAKAINEEMKVAASHALSALTKEDVPDSVLRAYGLESLKFGPDYIIPKPLDPRVLLWE
                     SPAVAKAAMDSGAARRHIDLEEYGEQLAYRQGMGEQVRYYIMHQAQKCPERKRVVFAE
                     GEESRIIRAAAQIVEEGIATPILIGRSDVVTQKIVALGLDCCPEIIDPNNFERIGEYA
                     QAYYEIRQRKGVVLQDAKKRVCEPNVFGPMMVAMGDADAFVSGLTYDYPEVLRPALQI
                     HHTHPGAERAAGVYIMIVDSRTYLFTDATVIIDPTAEDLAEIACLAADYARQLGLEPR
                     VALLSFSNFGSTPHPLSDKVRKAIQLIRDKCPDFAVDGEMQADTAVVSEIVDERYPFS
                     QVKDANVLVFPSLESANIAYKLVARLGNAEAIGPILLGVGAPIHVLQTGAEVRDIVNI
                     AAVAVMDAMNRKGCE"
     gene            complement(2845..3927)
                     /locus_tag="AC16V7_07240"
     CDS             complement(2845..3927)
                     /locus_tag="AC16V7_07240"
                     /inference="COORDINATES: protein motif:HMM:NF013752.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR6256950.1"
                     /translation="MESIITWLQSAIIKPVPILDLSSHPGKRRGKLRLSPEQLLTIIH
                     AQELAAWALGMSRRLSKPCLPRGPGGRPLTYPDSGILLMAVVQTTWRKSYEHMVDWVA
                     TNEALALVLGFRERTGEGKLQTISKGQYWERRQALGVLPFLFFFLALVAQLIRLGAIT
                     GKELIVDSTLLEAWYKADPGAAWQQYAGKAHVFGYKVHTLLCRQANLPVFVLITPANV
                     HDSLVGWLIVLVAALVYGFRVLVVYADAAYFDRRFFWVVFEILGAHAAVDYNLRRAGK
                     RKLAEPFFLRQWWRLVIRPRSDIERHFAWIKRYFGLKYFQCFTFLRVCQFVCLTYIAA
                     LAVALAAQRYDRPELVRSRAMVLAHV"
     gene            4302..4658
                     /locus_tag="AC16V7_07245"
     CDS             4302..4658
                     /locus_tag="AC16V7_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256951.1"
                     /translation="MGGLADFPPSDEPYELRDPVHLYVVNTGEKVMVIDPRIKTPSSY
                     PAKWNSQERKYIDPLTGTTDDLFGRPLRGPATQNLPRYPVEIKDGCVLIRLPWSQVEG
                     YEANAPAYRLPQLIKP"
     gene            complement(4644..6365)
                     /locus_tag="AC16V7_07250"
     CDS             complement(4644..6365)
                     /locus_tag="AC16V7_07250"
                     /inference="COORDINATES: protein motif:HMM:NF014044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6256952.1"
                     /translation="MMPDPQSPINNQQSTIGYVVGGGLKESLHVRLTIPAQQVQEGSF
                     VIIESGNWQFYGLVTDLLLGSTDPRFADEQSEARLPSNLARLLHGQTLYTNLEVLLAL
                     MLERGPDPGSPDYAQWLVDHPELPRPLPVKTVPSHHAPVRLAAEGDIAEIFGKPDDQG
                     NFVIGYTREQAHPVCINLDRFVQRSSGVFGATGTGKSFLTRLVLAGLIHYNRASMLVL
                     DMHNEYGFDDTASDTGLRVIGLKTKFPSRTRVVGLGQAALIRGKTPDFNLEVAMSDIQ
                     PQDIELLTRELNLRETTPTVLEALVSTYGREEWFSAFREMENGAFIQTDDGTKVPAPN
                     SVAHWAGRAGVNVMAAEGLHSKLNRLFHKSYIVEHPAADSVSEIIKSLEAGQHVVLSF
                     GKFESDLDYLLVSNLLTRRIRQAWEERTNAFRTTGKDEPRPLVIVLEEAHKLLNREMA
                     AQTTFSTIARELRKYYVTLLIVDQRPSQIYDEVMSQLGTRVSGWLGDDDDIRAVLSGL
                     AGRDALRGMLAHLQPKEEVLLLGWGVPLPLPVRSRRYDETFWDELLGKGGGKRSTSQS
                     LMELGFD"
     gene            complement(6362..6733)
                     /locus_tag="AC16V7_07255"
     CDS             complement(6362..6733)
                     /locus_tag="AC16V7_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015155778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR6256953.1"
                     /translation="MDADELKKRTKQFALRTIRLVETLPNTRTANVIGNQLLRSGTSV
                     GSNYRSACRGRSRAEFASKVGIAIEEADESLYWMEMLIETGIVQESHLSSLMQEANEL
                     IAIFTASVKTARSNNQRRQPK"
     gene            complement(6783..7397)
                     /locus_tag="AC16V7_07260"
     CDS             complement(6783..7397)
                     /locus_tag="AC16V7_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256954.1"
                     /translation="MRSIQIGRLLRASTSGFVAGCRVANFETDAPSLGALVRAPLGSS
                     MQIYGMIYDIHIDDDGLVRQLVTAEGVDEAAIADNRQNRTVPVEMSVLAVGYQQDGQI
                     YHLLPPRPPLSLDVVTLCDDEELRRFTSSGRFGYFRHILRNSDLPAAEILAAHIQQAQ
                     RAHLEHGNPAWVADASQELITLLRDDYTALMSVLSALSDTMIDF"
     gene            complement(7397..>7937)
                     /locus_tag="AC16V7_07265"
     CDS             complement(7397..>7937)
                     /locus_tag="AC16V7_07265"
                     /inference="COORDINATES: protein motif:HMM:NF020933.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HIR6256955.1"
                     /translation="ATVAGYVDKPGEDYVVRLLEVASQPAENARERPLRGVRDTDLFR
                     HLLVPGERSAVFEVQTRSAGIYRRRSASFAPHFFYLNVGRLGRPWLARVDILAWVAED
                     RHRLDDLHAVLVHQCRMLGSRPYPYALHRAHEVARVTADEKGQVELMITNELRRHGVP
                     VDESHKQYLKNLSGKGRYG"
BASE COUNT         1790 a         2099 c         2232 g         1816 t
ORIGIN      
        1 ctggatcacc aatatctttt ttggtaagcc atgtaattca tttaggcaag ttatccagga
       61 aataatgtga caaaagctac tatgcgggtg aagaaaatta tgcgaatatt attgcattga
      121 ctggagacca ttcttcgata cctgaaaacc ccaataccaa tcgtgaagga gttgaatatg
      181 accaaaataa cccgtgagga tgctctcgaa tatcatcgcc tgggtgggaa acctggtaaa
      241 gtttcggtgg ttccaaccaa accgatggcg acccagagag atttgagtct ggcatacacc
      301 ccaggcgttg ctgttccggt cttggaaatt gagaaagacc ctgagttagg ctatgaatat
      361 acttccaagg gaaacctggt tgcagttgtt tctaacggga cagccatact cggtttggga
      421 gaccgggggg cactggcgtc aaaaccggtt atggaaggca agggcgttct ctttaagcgc
      481 ttcgctgatg tggatgtttt cgatatcgag gttgacaccc atgatcctga cgaggtgatt
      541 caggttgttg cagccatttc tccgacattt ggcggcatca atttagagga tattaaagcc
      601 ccagaatgct tctacatcga agagaagctg aaagaaatgc tgaatatccc agtctttcat
      661 gatgaccagc acgggacggc aatcatctct ggcgcagctt taatgaatgg cttggaaatt
      721 gtgggtaaac gcatcgagga ggcccgaatt accatatcag gggcgggagc ttcggctatt
      781 tcgtgcgctg aactgatggt aaggcttggg gcacgccgcg ataatattct tctcgtcgat
      841 accaggggag ttgtctacca gggccgcacg gaaggtatga acaagtataa agagcgtttt
      901 gctcatgata cggatgcccg cacactagcc gatgccatgc gtggtgctga tgttttttat
      961 ggcctctcca ttgcgaatat cctgacaccc gaaatggtga aaacgatggc ggaaagtccc
     1021 ttgatctttg ccatggctaa tccagatccg gagattcgct atgaactggc gaaggaagcc
     1081 cgccctgatg cgattgtcgc tacaggtcgc tcggattacc caaatcagat taacaatgtg
     1141 cttggtttcc cgttcatatt ccgtggcgct ctggatgtgc gtgccaaagc gatcaatgaa
     1201 gaaatgaaag tcgctgcctc gcatgccttg tcagccttga ccaaggagga cgttccggat
     1261 agtgtgctgc gcgcatacgg gctggaaagc ctgaagttcg gcccggatta tattatcccc
     1321 aagccgctag acccgcgcgt cctgctgtgg gaatcgcctg cagtcgccaa ggcagccatg
     1381 gactccggtg cagctcgccg ccacatcgat ttggaagagt atggcgagca actagcctat
     1441 cgccagggga tgggggaaca ggtgcgttat tacatcatgc accaggctca gaagtgccca
     1501 gagcgcaagc gtgtggtctt tgcggaaggc gaagagtcta ggatcatccg ggccgcagcc
     1561 cagattgtag aggaaggtat cgcgactccg atcctgatag gtcgatcaga tgtagtcacg
     1621 caaaaaattg ttgccttggg tcttgactgc tgcccggaga ttatcgatcc aaataatttc
     1681 gagcggattg gggagtatgc acaggcttat tacgaaatac ggcaacgcaa gggggttgtc
     1741 ctccaggatg ccaagaagcg tgtgtgcgag ccgaatgttt ttggcccgat gatggtcgcc
     1801 atgggtgacg cagacgcctt tgtctctggt ctaacctacg attatcctga agtgctccgt
     1861 cctgctttgc aaatccacca cacccatccg ggtgcggagc gggcagcagg tgtgtatatt
     1921 atgatcgtcg atagccggac atacctgttt accgatgcaa ccgtgatcat cgatcccacc
     1981 gctgaggatc tggcggagat tgcttgcctg gcagccgatt acgcccgcca gctaggattg
     2041 gagccgcgcg tggccttact ctccttctcc aacttcggca gcacgcccca cccgctctcg
     2101 gacaaggtgc gcaaggcgat ccagctcatc cgtgataaat gccccgattt cgcagtggat
     2161 ggtgagatgc aagcggatac ggctgtcgtc tctgagatcg tcgatgagcg ctatcccttc
     2221 agccaggtca aagatgccaa tgtgctggtc ttcccatccc tggaatcagc caacattgcc
     2281 tataaactgg ttgcccggct gggtaacgct gaggcgattg gtcctattct cttgggtgtg
     2341 ggcgcaccca tccatgtctt gcaaacaggc gctgaagtgc gtgatattgt caatatcgcc
     2401 gcggttgcag tcatggatgc aatgaaccgc aagggatgtg aatagagaac agatcgtgtt
     2461 tattacgaag gactaagata ataatgaccc ccatttatgg gggtcattgc attttatgga
     2521 tatcgctgtg catcaattga ataggatgac gatatactta ctcgcttcag gctttcgatg
     2581 gtcatgtcca atgaacaagg aaatgtaagg gaaggctcca ggataacagc taaggttgtt
     2641 ggttagaaag ccaagagctg ggatgtctgg taccgctacg ccaaattggt cagttgtgag
     2701 tggttgtgta cataattaaa atgggtccat ctctacacaa tcacgcgcct tctgcacgcg
     2761 ggggattctt tgcctgaata ttatgtgctg taagcttcgt atttaaccaa tagcttatat
     2821 tgaaagcgtt cggaaacccg aagatcatac atgagctaaa accattgcac ggctgcgaac
     2881 cagttctggg cgatcataac gttgagccgc caacgcaaca gccaaggctg caatgtaagt
     2941 caggcagacg aactggcaca cacgcagaaa ggtgaagcat tgaaagtact tcaatccgaa
     3001 gtaacgtttg atccaggcga aatggcgctc gatgtccgag cgtggccgga tcaccagacg
     3061 ccaccactgg cgcaagaaaa atggttcagc cagcttgcgt ttgccagccc gacgcaggtt
     3121 gtaatccact gcggcatgag cgcccaggat ctcgaagacg acccagaaaa agcgccgatc
     3181 aaagtaggcg gcatcggcat acacgaccag cactcgaaag ccgtagacga gagcggccac
     3241 caacacgatg agccatccga ccagggaatc atgcacattg gctggggtta tcaggacaaa
     3301 cacgggcaag ttggcctgtc tacacagcag cgtatgcacc ttgtagccga agacatgcgc
     3361 tttcccggcg tattgctgcc aggctgcgcc tggatcggcc ttgtaccagg cttccagaag
     3421 ggtcgagtcc acgatcaatt ccttgccggt gatggctccc aggcggatca gttgcgccac
     3481 caacgccaga aagaagaaca ggaaaggcag aacgcccagg gcttgccgcc tttcccagta
     3541 ctggcctttc gagatcgtct gcaacttacc ttctccggtg cgctccctga agcccaggac
     3601 gagtgccaag gcttcattcg tcgctaccca gtccaccatg tgctcataag atttacgcca
     3661 ggtcgtttgc accactgcca tcaacaagat gccgctgtcc gggtaagtca gcggtctgcc
     3721 gccaggacca cgaggcaagc agggcttgct caagcgccgg ctcatcccca acgcccatgc
     3781 ggccagctcc tgagcatgga tgatcgtcag aagctgctcc gggctgaggc gcaatttgcc
     3841 acgccgcttg cccggatggg acgacaagtc caagatagga accggtttta tgattgccga
     3901 ttgcagccat gtaatgatag actccatgct gactactcct gcttatggga agatcgtggc
     3961 ggtacacgca tcttatccca tttgcctgag tagtcaatct tttaaggttc aatttgagag
     4021 tttccgaacg ctctctatac tatcttacaa ataataatcg attacatcat atgttttcct
     4081 tataattatg caaactctag aaaatcatac ttgatcagtc cgaatttttt tgtgtatgtt
     4141 atctagtgtt ggcatcacag gaggatgtat atcgactata tatagaatac ctatgcgtac
     4201 agaactggta tttctattgc tgtctagttt gctggtcttg agcgtgattt gcctggctgg
     4261 gttggcatgg tacgctaccc tcccataacg aggtgagtga tatgggcggc ctggctgatt
     4321 ttcccccgtc tgatgagccc tatgagctgc gcgatccggt ccacctgtac gtggttaaca
     4381 caggcgaaaa ggtgatggtc atcgatccaa ggatcaagac gccgagcagt tacccggcga
     4441 agtggaacag ccaggagcgc aaatatatcg atccactcac cggtacaacc gatgacttat
     4501 tcggccggcc tttgagggga ccagctaccc agaacttgcc acgctatcct gttgaaatta
     4561 aagatgggtg cgtcttgatc cgactcccct ggagccaggt ggagggatat gaggctaatg
     4621 cgccggctta ccggctaccc caactaatca aaccctaatt ccataagact ttgcgaggtg
     4681 ctacgcttac cccctccttt gcccagcagc tcgtcccaga atgtctcatc atagcggcga
     4741 gagcgcacag gcaagggtag cggtacgccc cagcccagaa gcagaacttc ctctttgggt
     4801 tgcagatgtg ccagcatgcc acgcaaggcg tctctgccag ccagaccgga gagcacggcg
     4861 cggatgtcgt catcgtcgcc caaccagccg gagacgcgcg tgcccagctg cgacatgacc
     4921 tcatcgtata tctgcgaggg gcgctggtca acaatcagca gggtgacata gtacttgcgc
     4981 agctcgcggg cgatggtgct gaaggtggtc tgggcggcca tctcgcggtt gagcagcttg
     5041 tgggcctcct ccaggacgat gaccagcgga cgcggctcat ccttgccagt ggtgcgaaag
     5101 gcgttcgtgc gctcctccca ggcctggcgg atgcgtcggg tgagcaggtt gcttaccagg
     5161 aggtagtcca ggtcgctctc aaatttcccg aaagaaagca caacatgctg tccggcctct
     5221 aaagatttga tgatttcgct cacgctgtct gcagcggggt gttcgacgat gtagctcttg
     5281 tggaatagac ggttcagctt ggagtgcaga ccttcggcgg ccatgacgtt cacccctgcg
     5341 cggccagccc agtgagccac gctgttcggc gcaggcacct tcgtgccatc gtcggtctgg
     5401 atgaacgcac cgttttccat ctcgcggaag gccgagaacc actcctcgcg cccataagtg
     5461 ctcaccaagg cttccaggac agtcggcgtg gtctcccgca ggttcaactc gcgggtgagc
     5521 agctcgatat cctggggctg gatatcgctc atggcgacct ccaggttgaa gtcaggggtt
     5581 ttgccacgga tgagagccgc ctgtcccagg ccgaccaccc gggtgcggct ggggaatttg
     5641 gtcttgagac cgatgacgcg cagaccggtg tctgaggcgg tgtcgtcgaa gccgtattca
     5701 ttgtgcatat ccaataccag cattgaggcg cggttgtagt ggatcaaacc agccagcacc
     5761 agccgagtga ggaaggattt gcccgtcccc gtggcaccga atacaccaga cgagcgttgc
     5821 acgaagcgat ccaggttgat gcaaaccggg tgtgcctgct cgcgggtata accgatgacg
     5881 aagtttccct gatcgtcggg tttaccgaag atttcagcga tatcgccctc cgcagccagc
     5941 ctgaccggcg catggtgtga cggtacggtc ttgactggca gcgggcgggg gagctcggga
     6001 tggtcgacca gccattgcgc gtaatccggt gagccggggt cgggcccgcg ttccagcatc
     6061 agcgccagca acacttccag gttggtgtaa agggtctgcc cgtggagcaa acgggcaaga
     6121 ttggacggca ggcgcgcctc gctttgttca tcggcaaagc gcgggtcggt ggagcccaat
     6181 agcaggtcgg tgaccagccc gtagaactgc cagttcccgc tctcgatgat gacgaagctg
     6241 ccttcctgca cctgctgggc ggggatggtc aagcgcacgt gcaggctttc cttcagcccg
     6301 ccgccgacaa cgtagccgat tgttgattgc tgattattga ttggtgattg aggatctggc
     6361 atcattttgg ttgccttctt tggttgttac tcctagcggt tttaacagag gcggtgaaaa
     6421 tggcgattaa ttcatttgcc tcttgcatca acgagcttag atggctctcc tggacaatac
     6481 ccgtttcgat aagcatctcc atccaataca aggattcgtc tgcttcttcg atggcaatac
     6541 ccactttgga tgcaaattcg gctcttgagc gtccccggca tgcagaacga tagtttgatc
     6601 caactgaggt gcctgatcga agcaactggt tgccgatcac gttcgcggtt cgagtgttgg
     6661 gtagggtctc cactaatcga atcgtacgta aagcgaattg ttttgtgcgt ttttttaatt
     6721 catccgcgtc cattgagcct ctaaaattgt caatagacca atcaacaatc atcaatttgc
     6781 aattaaaaat ctatcattgt gtcacttaat gcacttagta cactcatcag tgcggtgtag
     6841 tcgtcgcgca gcagagtgat gagctcctgt gaagcgtcgg cgacccaggc gggattgccg
     6901 tgctcaaggt gcgcacgctg cgcctgttga atgtgtgccg caagaatttc ggcggcgggc
     6961 aggtcgctgt tacgtaggat atgccggaag tagccaaagc gtccactgga tgtgaagcgg
     7021 cgcagttctt cgtcgtcgca cagggtgacc acatccaggc tgagaggcgg gcgcggcggc
     7081 aggagatggt agatctgccc atcctgctgg tagcccaccg ctagcacgct catctcgacc
     7141 ggcacggtgc ggttttgacg gttatcggcg atggcggctt catccacccc ttcggcggtc
     7201 accaattgcc gtaccaggcc atcgtcgtcg atgtggatgt cgtaaatcat gccgtagatt
     7261 tgcatgctgc tccccagcgg agcgcgcacc agcgcaccca aagagggagc gtctgtctca
     7321 aagttcgcca cgcggcagcc cgccacaaag ccactggtac tagcgcgcag caatcgcccg
     7381 atctggattg atctcattag ccgtatctcc ccttgccgga taagttctta agatattgtt
     7441 tgtgagattc atcaaccggc accccgtgcc tgcgcagctc attggtgatc atcagctcca
     7501 cttgtccttt ttcatctgct gtcacccggg cgacctcgtg agcgcggtgc aatgcgtagg
     7561 ggtagggacg tgagccgagc attcggcact ggtgaaccag cacagcgtgc aggtcatcca
     7621 gcctgtgcct atcttcagcc acccaggcga ggatatcgac gcgcgccagc cagggacggc
     7681 caagcctgcc cacgttgagg tagaagaaat ggggtgcgaa ggatgcgctc ctgcgccggt
     7741 agatgccggc ggagcgcgtc tgcacttcga atactgcaga gcgctcaccc ggtaccagga
     7801 gatgccggaa gaggtcggta tcgcgcacgc cgcgcagtgg acgctctctg gcgttttctg
     7861 cgggctgaga agctacttcc agcaggcgga ctacatagtc ctctcctggt ttatccacgt
     7921 atcctgccac ggttgcg
//
LOCUS       DBNHOH010000410         1450 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_921313, whole genome shotgun sequence.
ACCESSION   DBNHOH010000410 DBNHOH010000000
VERSION     DBNHOH010000410.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_921313"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..>1450
                     /locus_tag="AC16V7_07270"
     CDS             131..>1450
                     /locus_tag="AC16V7_07270"
                     /inference="COORDINATES: protein motif:HMM:NF028199.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HIR6256956.1"
                     /translation="MRSVSKLEIAIKALRELGLGQVSQYAWYKILLRTGYIRWATRNP
                     ALKTAGARRDQSRPRGLSGVRPLFRLPAREQVMLTLGESGLADLLKEADEILSGQVRL
                     FGGEPQPLNLSPPGPLLHWTRYDPGRGEGGDAQDIKFLWEPGRFGWAYILGRAYYLSQ
                     DERYAEGFWKFTETFWEANPPYLGPHWVSAQEVALRLLALVFAYQIFSPSIHTTTPRS
                     VLIYQIIADHARRIPPSMTYARAQNNNHLLSEAVGLYTAGLALPNHPKAVRWQKMGWR
                     WIQRGLQKQIAEDGAYIQHSTNYHRLMLQLALWVHALPETQRQPFPAATRQSLSAATR
                     WLLELVDGESGRVPNLGPNDGAYIQRLTVCPFHDFRPVLQAAGSAFLGERPYPPGLWD
                     EMSLWVGDVGQGIRDGGDARGDDVRLSSPPVLRIPQQESWAYLRAARF"
BASE COUNT          295 a          386 c          422 g          347 t
ORIGIN      
        1 tcatagccgc catttccgct ggtggcccgc cccctattcc ttacgatcat attattgggg
       61 tgacgcaggc gaccttcgcc gcagtcgagg ccttgcgaaa caatgaacgc aggttggtaa
      121 aatttcagga atgaggtcgg tttctaagct agaaattgca ataaaagcgt tgcgggagct
      181 gggtctaggt caggtgtcgc agtatgcctg gtacaagatc ctgctgcgaa cgggttatat
      241 ccgctgggcg acgcgcaatc ctgctctaaa aacggcgggt gctcgccgtg atcaatctcg
      301 tccgcgcggt ttgtccggtg ttcgcccatt attccgcctg cctgcaaggg agcaagtgat
      361 gctgaccttg ggtgagagtg gtttggcgga cttgctaaag gaggcggacg agatcctttc
      421 tgggcaggtt cgattgtttg ggggtgagcc gcagccattg aatttgagcc caccgggccc
      481 tttgcttcac tggactcggt atgaccccgg gagaggggag gggggagatg cacaagatat
      541 aaagtttctc tgggagccag ggcgcttcgg ctgggcatat attctggggc gtgcctacta
      601 cctcagccag gatgagcgat atgccgaagg tttttggaaa tttacggaga cattttggga
      661 ggccaacccg ccttatttgg gtccgcattg ggtctcagct caagaggtcg ctttgcgatt
      721 gctcgcattg gtctttgctt accagatttt ttctccttct atccacacca caaccccgcg
      781 ctctgtgctc atctaccaga taatcgctga ccacgcccga cgcataccgc ccagcatgac
      841 ttacgcccgg gcgcagaata ataaccactt actcagcgag gcggttggct tgtacactgc
      901 tgggctggct ctccccaacc accctaaagc agtgcgctgg cagaagatgg gctggcgctg
      961 gatacaaagg ggcttgcaaa agcaaatcgc tgaagatggc gcttatatcc agcacagcac
     1021 aaattaccat cgtttgatgc tgcaacttgc cctgtgggtg catgcccttc ctgaaaccca
     1081 aaggcaaccc ttccctgctg ctacgcgcca aagcctgtca gcagccaccc gctggttgct
     1141 ggagctggtt gatggtgaaa gcgggcgcgt ccccaattta ggaccgaacg atggcgccta
     1201 tatccaacgt ctcactgttt gccctttcca tgatttccgc ccggtgctgc aagccgccgg
     1261 atctgctttc ctcggtgagc gaccatatcc tcctgggtta tgggatgaga tgagtctttg
     1321 ggtaggggat gtgggacagg ggataaggga tgggggagat gcaagagggg atgatgtccg
     1381 gctttcgtcc cctcctgtat tgcgtatccc tcaacaagaa tcatgggcct acttgcgtgc
     1441 agcacgcttt
//
LOCUS       DBNHOH010000411         3906 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1112775, whole genome shotgun sequence.
ACCESSION   DBNHOH010000411 DBNHOH010000000
VERSION     DBNHOH010000411.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1112775"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..1138)
                     /locus_tag="AC16V7_07275"
     CDS             complement(209..1138)
                     /locus_tag="AC16V7_07275"
                     /inference="COORDINATES: protein motif:HMM:NF024637.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HIR6256957.1"
                     /translation="MMHKYSLVILVIMIIFAAYAPLTPAFAQDEIKLSNLEVDLWPEY
                     DRPSVLVIYRITLPPTISLPVDLTFRIPAAAGDPSAVALRQMTPEGDSGLFTIPYQRQ
                     VVGEWGMITITATVPEIQLEYYDPDLVKQDDARQFEYRWPGDYAIDALILQVQQPLGA
                     SEMVISPASDPGVSGNDGLVYYNKQVGSLAAGQTFSLTVNYNKASDDLTAANLQVQPS
                     APVSVTPGQNSLMTLLPWALGILGVLLILGGVVWYRQSGRGKPGDRPRRRRATASREP
                     EESTSFIYCHQCGKRASPGDRFCRTCGTKLRTE"
     gene            complement(1261..2028)
                     /locus_tag="AC16V7_07280"
     CDS             complement(1261..2028)
                     /locus_tag="AC16V7_07280"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6256958.1"
                     /translation="MNSSADKPIVAINHLSAIFDNGNGGLRALEDISFSVSSQEFVSV
                     LGPSGSGKSTLLRILAGLLPPTRGQVFYQGEELSGPRRGVGFVFQRANLMPWRTVLEN
                     ITLPLELLGTPHQTTLERAQELVNLVGLQGFEDSLPRDLSGGMAQRVAIARALIHDPD
                     VLLLDEPFGNLDALTRERMGAELLRIWQARRKTVIMVTHSISEALFLADRVLVLTPRP
                     GRLRLDLSVELPRPREDDVRYTPVFGKLVRQVREAIG"
     gene            complement(2009..3022)
                     /locus_tag="AC16V7_07285"
     CDS             complement(2009..3022)
                     /locus_tag="AC16V7_07285"
                     /inference="COORDINATES: protein motif:HMM:NF020650.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6256959.1"
                     /translation="MAHKTILTVALVFLLALAACSPSATPSQETTESPQETEPPLAHI
                     RLPLGYIPNIQFAPLYVAVEKGYYQAEGIEIEFDYSFETDGVSLVGAGELQFAVVSGE
                     QVLLARAQELPVVYVMAWYQDYPVGVVAKTSQSIRTPQDLAGKKIGLPGPFGASYIGL
                     RALLNAGGLQESQVTLDSIGFNQVEALAADQEQAVVIYIANEPVQLRAKGYEVDEIRV
                     SDYVRLASNGLITNEATIQQNPDLVRAMVRATLRGIADVIANPDEAYKISLEYVEELA
                     QADEAIQKEILATSITFWQAEQLGVSDISAWENMQAVLLDMGLLAQPLDLSKAFTNEF
                     ISR"
     gene            complement(3057..>3906)
                     /locus_tag="AC16V7_07290"
     CDS             complement(3057..>3906)
                     /locus_tag="AC16V7_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6256960.1"
                     /translation="VPKLWGALFDRMNMDITRTEKTLPWPVRNLGRSRIYLALFSMLL
                     TLLLWGAVTSLTDFPAFILPGPWLVWARFWQLVIDGSLLRHILVTLQEVLLGLALGVS
                     AATVLGYLLAKSPVFERLLSPYIVASQSVPVVAIAPLLIIWFGPGMFSKILICALIVF
                     FPVLVNTIVGLRSVPEELEDLMRSLQATRWQMAAYLQVPAALPVFLGGLRIGATLSVI
                     GAVVGEFVGADRGLGFLINLGRGQYDTALVFVAVFTLVTLALGLYGAVVVIEYRWLSW
                     QQRIEL"
BASE COUNT          871 a         1072 c         1135 g          828 t
ORIGIN      
        1 gacggaaaaa cgggttattg ggtggcggta ccgccgccca ctaacccgtt tttccgttat
       61 ttcccccgcc ctcccggcgg gagggcgggg gaaataacgg aaaaacgggt tattgggcgg
      121 cggtaccgcc acccaataac ccgtttttcc gtcaatcagg cgaaataccc gaaatttatt
      181 attcggggtg ttagatgttt tttgagggtt actcggttct taattttgtg ccgcacgtgc
      241 ggcagaagcg gtctccaggc gaggcccttt taccacattg gtggcagtag atgaaactgg
      301 ttgattcttc tggttctctc gaggctgtcg cccggcgtcg tcgagggcga tcgcctggtt
      361 tgcctctgcc tgactgccgg taccaaacta caccgccaag gatcagcagt acccccagga
      421 tgcccagagc ccagggtaag agggtcatca agctgttttg acccggcgtg acactgacgg
      481 gcgcgctggg ctgcacctga agattagcgg cagtcagatc atcagaggct ttgttgtagt
      541 tgactgtcag cgagaaggtt tgcccagccg cgagggagcc aacctgcttg ttgtaataca
      601 ccagcccatc attgccggac acacccgggt ctgatgcagg cgagatgacc atttcgctgg
      661 cacccagcgg ttgttggact tgtaaaatta gcgcatcaat ggcgtaatct cccggccagc
      721 gatactcgaa ctggcgagcg tcatcctgtt taactaaatc cgggtcgtaa tactcgagct
      781 ggatctcggg tacagtggct gtgatggtta tcatgcccca ctccccaacg acctggcgtt
      841 ggtaagggat tgtgaataac cctgaatcgc cctcgggagt catctggcgc aatgccacgg
      901 cactcgggtc acccgcggcg gccgggatgc gaaatgtcag gtcaactggc agagatatag
      961 tggggggcag cgtgatgcga tagatgacta agacagatgg acgatcgtat tcaggccaca
     1021 ggtccacttc tagattgctc agcttgatct catcttgagc gaaggctggc gtgagcggcg
     1081 cgtaggcggc gaagatgatc atgatcacca gaataacaag ggaatattta tgcatcattt
     1141 cttcctcgta atgaaattcg gttgggaatc ttatcatgca atgaggcata cggggagggc
     1201 agagttagcg gaaaggggac ttatacccat ttccgaggta gattcccagg aagttaggga
     1261 ctacccaatt gcttcgcgta cctgccgcac cagcttgccg aagaccggag tgtagcgcac
     1321 atcgtcctcg cgaggtcggg gcagttcaac gcttaagtct aggcgcaagc gcccggggcg
     1381 aggtgtgagt accaggacac ggtcggccag gaaaagcgcc tcagagatcg agtgcgtgac
     1441 cattatcacg gtcttgcgcc ttgcctgcca gatgcgcagc agctcagctc ccatgcgctc
     1501 gcgcgtcaag gcatccaggt tgccgaatgg ttcatctagc agcagcacat ctggatcgtg
     1561 aatcagggcg cgggctatgg cgacacgctg cgccataccc ccggagaggt cgcgcggcag
     1621 gctgtcctcg aaaccctgca atccgacgag attgacgagc tcctgggcgc gttccagggt
     1681 tgtctggtgg ggtgtgccca gcagctccag cggaagagta atattttcca ataccgtacg
     1741 ccagggcatc aggttggcgc gctggaacac gaatccgacc ccgcgccgtg gtccgcttaa
     1801 ctcttcaccc tgataaaaaa cctggcctcg ggtgggaggc aataaacctg cgagtatgcg
     1861 aagcagggtg ctcttgccgc tgccagacgg accgaggacg ctgacaaact cctgtgagct
     1921 cactgagaag gagatatcct ccagcgctcg caagcctccg ttgccattgt caaaaatggc
     1981 gctcaggtgg ttgatggcga cgatgggttt atcggctgat gaattcattc gtgaaagcct
     2041 tgctcaaatc cagcggttgg gcgagcagcc ccatatccag cagaacagct tgcatgttct
     2101 cccaagcgga aatgtccgat acgcccaatt gttcggcttg ccagaatgtg atggatgtgg
     2161 cgaggatctc tttttggatc gcctcgtctg cctgagccag ctcttctacg tattcgaggc
     2221 tgattttgta cgcctcgtct ggattggcta tgacatcagc aataccgcgc agtgttgctc
     2281 tcaccatggc gcgcaccagg tctggatttt gctggatggt ggcttcgttg gtgattagcc
     2341 cgttggatgc caggcggacg taatcggaga cgcggatttc atccacttca tatcctttgg
     2401 cgcgcaattg aaccggctca ttggcgatat aaatgaccac tgcctgctcc tggtctgcgg
     2461 ccagcgcttc gacctggttg aagccgatcg aatccaaagt tacctggctc tcttgcaacc
     2521 cgcctgcatt gagcaaggcg cgcaagccaa tatagctggc tccgaatggt cctggtaggc
     2581 cgatcttctt tcccgccaga tcctgcggag tgcggatact ctggctggtt tttgccacca
     2641 cacccaccgg gtagtcctgg taccaggcca tcacatagac gactggtagc tcctgggcgc
     2701 gcgccaacag cacttgctcg ccggagacca cggcaaattg tagctcgcct gcccccacca
     2761 gggagacgcc gtcggtctca aagctgtaat cgaactctat ctctatgccc tcagcctggt
     2821 aatagccctt ttctacagcg acgtacaggg gcgcgaactg gatattgggg atgtagccca
     2881 gtggtaggcg aatgtgcgcc agaggtggct cggtctcctg cggtgattcg gttgtctctt
     2941 gtgagggggt cgcgctcggc gagcaagctg ccagcgctag taggaagacg agagcaacag
     3001 tgagtatcgt tttgtgtgcc atccgaaaaa tcctcccaaa atctatctgg ctgatcctat
     3061 agctctatgc gttgttgcca ggaaagccag cggtattcga taacaacgac ggcgccgtac
     3121 aggcccagcg ccagggtaac cagcgtgaag actgccacaa ataccagggc ggtatcgtac
     3181 tggccgcgcc caaggttgat caaaaagccc aaaccgcggt ctgcgccgac gaactcaccc
     3241 actactgcgc cgataaccga gagagtggcc ccgatgcgca ggcctcccag gaagactggc
     3301 aaggcggcag gcacttgcag gtaggcggcc atttgccagc gggtggcttg cagcgagcgc
     3361 atcagatctt ccagctcctc cggtacagag cgcagtccga cgatggtatt gaccagcacg
     3421 gggaaaaaga cgatcagggc gcagatcagt atttttgaga acatgcctgg cccaaaccag
     3481 atgatgagca gtggggcgat agccacaaca ggcacagatt ggctggcgac gatgtatggc
     3541 gagagcagtc gctcaaatac cggcgatttt gccaacaggt agcctaagac tgtagcagcg
     3601 ctcacaccca gcgccagccc taaaaggact tcttgcaatg tcaccaaaat atgccgcagc
     3661 aggctgccat caatgacaag ctgccagaag cgcgcccaga ccagccacgg gccagggagt
     3721 atgaaggctg gaaaatccgt caggctcgtt acagcacccc ataacaggag cgtaagcagc
     3781 atcgaaaata gtgccagata tatcctggag cgtcccaaat tgcgaaccgg ccaaggaagt
     3841 gttttttctg tgcgcgtgat atccatattc atcctgtcaa ataaagcgcc ccagagttta
     3901 gggact
//
LOCUS       DBNHOH010000412         1562 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_856792, whole genome shotgun sequence.
ACCESSION   DBNHOH010000412 DBNHOH010000000
VERSION     DBNHOH010000412.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_856792"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /gene="hydA"
                     /locus_tag="AC16V7_07295"
     CDS             <1..1205
                     /gene="hydA"
                     /locus_tag="AC16V7_07295"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HIR6256961.1"
                     /translation="DHYTGHKAAAFGGTTTVMDFVPQERATLKENVELWRAKADPKAA
                     IDYSFHMNSTHFSEAIAEQITSLVDLGITSVKVFTAYNNRLRLMDGDIFRVMRIAKEN
                     GFLTMLHAENGDVIEVLVEEALAAGHTSPEWHALTRPTWGAVESALRGIALTAETGAP
                     LYIVHMNAAGEVDQLSYGRERGLPVMGETCPAYLFFTIDHLRRPDGAKWICSPPMRSE
                     ADNARLWEGIADGTIQTIGTDHCPFFYDGTKPILYENKPIAIPGKELGKDDFTKIPNG
                     LPGVGDRLPILWTYGVRAGRMTANQFVALNSTNPAKIFGLYPRKGALLPGSDADIVIW
                     DPERRLTYGLAHAQHRTDYNLYEGWELVGFPEKVFLRGQMIVDGENWLGKPGIGQFIP
                     RQRGEVIV"
     gene            1272..>1562
                     /locus_tag="AC16V7_07300"
     CDS             1272..>1562
                     /locus_tag="AC16V7_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256962.1"
                     /translation="MISYLPLFSILVLLIFSALTPAAFLFRRSMTQSWLVAVLGAFIG
                     WILILLAGFDLPYNISLLSWEPPELFSASPLLLVDRISWPFALGLATLALAVI"
BASE COUNT          372 a          369 c          419 g          402 t
ORIGIN      
        1 atgaccacta cacaggtcac aaggcagcag catttggcgg aaccacaact gtgatggatt
       61 ttgtgccaca ggagagagcc acgctgaagg agaatgtgga gctttggcgc gccaaagctg
      121 accctaaagc tgccatcgat tatagcttcc acatgaatag tacccatttt agcgaagcga
      181 ttgctgagca aataacgagt ctggttgacc tgggtattac gagcgtgaag gtatttaccg
      241 cctacaacaa ccgcttgcgg ctaatggatg gggacatatt ccgtgtgatg cgcatagcca
      301 aagagaatgg gttcttgacg atgctgcatg cggagaatgg cgatgtgata gaagtgttgg
      361 ttgaagaggc tctagcggca gggcacacct cacccgagtg gcacgcgctc acgcgtccta
      421 cctggggagc agtagaatct gccctgcgcg ggatcgcatt gactgccgag accggcgcac
      481 ccctttacat tgttcacatg aatgctgccg gagaagtaga ccagctgagt tacgggcggg
      541 aacgaggtct tccagtgatg ggggagacat gcccggcata tttgttcttt accatcgatc
      601 atctgcgacg accagacggg gcaaagtgga tttgctcgcc tcccatgcgc tcggaagcag
      661 ataacgctcg cttatgggaa ggcatcgccg atggtactat ccaaactata gggacagacc
      721 attgcccgtt cttctacgat gggacaaaac ctatacttta tgaaaataag ccgattgcaa
      781 ttcccggtaa ggagttgggc aaggatgatt tcacgaagat acccaatggg ctaccagggg
      841 taggagatcg tttgccaatc ttatggacat atggggtcag ggctggacgg atgacagcca
      901 accagtttgt tgccctaaac agcacgaacc cggcaaaaat ctttggtcta taccctcgaa
      961 aaggcgcctt gctccctggt tctgatgctg atatcgtcat ctgggatcct gagcggcgct
     1021 tgacctatgg cttggcgcat gcccagcacc gcacagatta caacttgtat gaggggtggg
     1081 agttagtagg ctttcctgaa aaagttttcc tgcggggtca aatgattgtg gatggtgaaa
     1141 attggttggg aaaacctggt atagggcaat ttatccctcg ccaacggggt gaagtaatcg
     1201 tgtaatacca aacagaattc ataagggaaa tcaatcaatt ccgatctaaa cccgttgtcc
     1261 accattcttt catgatttct tatctgcctc tgttttcaat tcttgtcttg ctcatcttct
     1321 cggcactaac gccggctgcc ttcttgtttc ggcgtagcat gacgcaatcc tggctggtgg
     1381 cagtcttggg ggcctttatc ggatggattc tcattttact tgcaggtttt gatctgccat
     1441 acaatatttc gctattaagt tgggaaccac cggagttgtt ctcagcatcg cccttattgt
     1501 tggttgatcg aatttcctgg cctttcgcct taggtttggc aacgcttgct ttggcagtca
     1561 tt
//
LOCUS       DBNHOH010000413         2747 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_664730, whole genome shotgun sequence.
ACCESSION   DBNHOH010000413 DBNHOH010000000
VERSION     DBNHOH010000413.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_664730"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /gene="dnaX"
                     /locus_tag="AC16V7_07305"
     CDS             complement(<1..1040)
                     /gene="dnaX"
                     /locus_tag="AC16V7_07305"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIR6256963.1"
                     /translation="MAQALYLKWRPHRWEEIVGQPHIVQTLRNAVAADRVAHAYLFAG
                     QRGTGKTTTARLLAKAVNCLEEELASRPCDRCAHCKAVNKGRFLDLIEIDAASNTSVD
                     DVRSLRDKINFSPNQGRYKVYIIDEVHMLSTAAFNALLKTLEEPPPHAIFILATTEIQ
                     KIPATVLSRCQRHEFRRIPVNEIVAHLKQIVAAEGLQTEDEALTLLARQATGALRDAI
                     SLLDQLASTGQAITLERAQQVLGTATSQSVLEIVDAIQSNQSAAGLDYIHATLDAGSD
                     PRQFARQIVDYLRNLLLVRMGNPEQLDATADLKSQMARHAQGFDVPQLLSLIRVFNQA
                     ANDARSAWQPSLP"
     gene            complement(1048..1497)
                     /locus_tag="AC16V7_07310"
     CDS             complement(1048..1497)
                     /locus_tag="AC16V7_07310"
                     /inference="COORDINATES: protein motif:HMM:NF020908.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ family domain-containing protein"
                     /protein_id="HIR6256964.1"
                     /translation="MSNIEEHIRRAMQEGQFDNLPGKGKPLHLDDNPFEDPEWHLAYH
                     VLRNSGFSLPWIEKRRELEEAIEAARKALRIKWSWRESALASNQPLAAINAEWSRARQ
                     NFCRQVDELNQQIMSYNLEAPSERFHMPRLNAEREITLTTSPGSDKL"
     gene            complement(1530..2321)
                     /locus_tag="AC16V7_07315"
     CDS             complement(1530..2321)
                     /locus_tag="AC16V7_07315"
                     /inference="COORDINATES: protein motif:HMM:NF013125.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR6256965.1"
                     /translation="MRQWKRLVYYLLINILVSACTVLAVLMIWDRTQTPATLLTPEPS
                     IPVISALSTSAPTQEGTPPTQETPGPTSSPTSKPSPIGDVEEYQVGFGDTLGQISDRF
                     DVPIEELMRVNQINDPNALSVGMVLYIPVTPQARPSDTPAPTSTSATLGTTLPTNPPQ
                     ETRLIINAVISPGELTSEHVFITRTGDGPLSLAGWQLKDEDGNVFVFPQLDLYKDGAV
                     NVWTTSGANTVVDLYWGLSAPIWKSGEKVTLLDANGKERATYTVP"
BASE COUNT          539 a          713 c          863 g          632 t
ORIGIN      
        1 ggcagggaag gctgccatgc gctgcgggca tcattggcgg cctggttaaa aacgcggatc
       61 aaacttaaca gctgtggaac gtcgaagccc tgggcgtggc gggccatttg cgacttgaga
      121 tcagcggtgg cgtccaactg ctccgggttg cccatccgca ccagcagcag gttgcgcagg
      181 tagtccacga tctggcgggc aaactggcgc gggtcgctgc cggcatccag tgtggcgtgg
      241 atataatcca gcccagcggc gctctggttg ctctgtatgg catccacgat ctccaggacg
      301 gattggctgg tggcggtgcc gagcacctgt tgggctctct ccagcgtgat ggcctgacct
      361 gtggaagcga gctgatccag cagtgagatg gcgtcgcgca gggcaccggt agcctgccgg
      421 gcaagcaagg tcagggcctc gtcttcggtt tgcaagccct ccgcggcgac gatctgcttg
      481 agatgggcga cgatctcatt gaccgggata cggcggaact cgtggcgctg gcaacgtgaa
      541 agcaccgtag cgggtatttt ttggatttcc gtggttgcca ggatgaagat agcgtggggg
      601 ggcggctctt ccaatgtttt cagcagggcg ttaaaggctg ctgtggagag catgtggact
      661 tcatcaataa tgtagacttt gtagcgaccc tgattggggg agaagttgat cttgtcccgc
      721 aggctgcgca cgtcatccac gctggtattc gaggcggcgt cgatctcgat tagatcgaga
      781 aagcggccct tgttaactgc cttgcagtgg gcgcagcgat cgcacgggcg cgaagctagc
      841 tcttcctcca ggcagttgac cgcctttgcc agcaagcggg cggtggtggt tttgcccgtg
      901 ccgcgctgac cggcaaacag gtaggcgtga gcgacacgat ccgccgccac cgcgttgcgc
      961 agggtctgga caatgtgcgg ctgaccgacg atctcttccc agcggtgagg tcgccatttt
     1021 agatacagtg cttgagccat aagatgttta gagtttatca gagccaggcg atgtggtcaa
     1081 cgttatctcg cgctcagcgt tcagccgggg catgtgaaag cgctcggacg gggcttccag
     1141 gttatagctc atgatctgct ggttgagctc gtccacttgc cggcagaagt tttgcctggc
     1201 acgcgaccat tcagcgttga tggctgccag gggctgattg ctcgccaggg ctgattcccg
     1261 ccatgaccat ttgatgcgca gcgccttgcg cgccgcttcg atggcctctt ctagctcgcg
     1321 gcgcttctcg atccagggca agctaaagcc gctgtttcgc aatacatggt acgccaggtg
     1381 ccattcaggg tcttcgaaag ggttatcgtc caggtgcaag ggtttccctt tgcctgggag
     1441 gttatcgaac tggccctcct gcatggcgcg gcggatatgt tcttcgatat tgctcatgac
     1501 acttaaaaaa accctggata catcgactgt taggggacgg tgtacgtggc tctctctttg
     1561 ccattggcgt cgagcagggt tactttctcc ccagatttcc atatgggggc ggacaatccc
     1621 caatacaaat ctacaacggt gtttgctccc gaggtggtcc acacattgac tgcaccgtct
     1681 ttatataaat ccagttgggg gaagacgaac acgttgccgt cttcatcctt caattgccag
     1741 cctgccaggg aaagaggccc atcgccggtg cgggtgatga agacatgctc ggaggtcaat
     1801 tcgccggggc tgatcacggc attgatgatc aggcgcgttt cctggggcgg gttggtgggt
     1861 agcgtggtgc ccagtgtagc tgaggtactg gtgggggctg gcgtatcgct cggcctggct
     1921 tgcggggtga ccgggatgta aagcaccatg cccaccgata aggcgttggg atcattgatc
     1981 tggttgaccc gcatcaactc ctcgatgggc acatcaaagc gatcggaaat ctgccccagc
     2041 gtgtcgccaa atcccacctg gtactcttcc acgtctccaa taggcgacgg tttgctcgtt
     2101 gggctggaag tgggaccagg cgtttcctgc gtgggggggg tgccttcttg ggtaggtgca
     2161 gacgtgctga gcgcggagat aaccgggatg gatggctcag gagtaagcag tgtcgccggg
     2221 gtttgtgtgc gatcccagat catcagcacc gctaggacgg tgcaagcaga taccaggatg
     2281 ttgatcagca ggtaatagac caggcgtttc cattgtctca tggcggggcg ctcctgtgaa
     2341 atcatagcat aatctataga aactcacaac cagagttttc aaaagaacgg ggttttggga
     2401 tgcaggaagg cggctccacc gccttcctgc acccccatac tccccttggg atttcgcact
     2461 tatgaaaccc ctgaaaggca caacgttagg gtaatcgcat tagggcgatt tctctctttg
     2521 cgggattgca tatgccctgg cacaacgttg acggacgggc agtttgtcaa taaaatggaa
     2581 tgcgtatgcc atatctgatt gacggacata atctgatccc caagattcct gggctggatc
     2641 tcggggatgt ggatgatgaa attcaacttg tcgagttgtt gcaagaattt tgccgcaacc
     2701 gccgcaagca ggtggaagta tattttgaca acgctccgcc tgggcag
//
LOCUS       DBNHOH010000414         3545 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1177516, whole genome shotgun sequence.
ACCESSION   DBNHOH010000414 DBNHOH010000000
VERSION     DBNHOH010000414.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1177516"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="AC16V7_07320"
     CDS             complement(<1..377)
                     /locus_tag="AC16V7_07320"
                     /inference="COORDINATES: protein motif:HMM:NF013482.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR6256966.1"
                     /translation="MAIGKSIPMLDALARVTGTVDYMINLQLPEMLIGKIVRSQSPHA
                     NLLKVDTTEALRVPGVVAILTGADLGPNSLYGASIKDQGVVAIDTVRYVGEPIAAIAA
                     ESEEAAEEAAMLLDIEYDELPAV"
     gene            complement(364..1224)
                     /locus_tag="AC16V7_07325"
     CDS             complement(364..1224)
                     /locus_tag="AC16V7_07325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR6256967.1"
                     /translation="MSIDFLEPVDFSEAVALLSEYGDDAKVIAGGTSVVLMLQQKLIA
                     PSVLISLGRISGWDFVRTEPDGLHIGALIPLHDAELSPVVRQFFPALAHTFSVVGNVR
                     VRNQATLGGNLSAADYAADPPAMLTALGARARLISLEGTRDVPLSEFFLGFYTTALNP
                     TEILAEVVIPTLPETTRAAYLRYTSVSAEGRPCVAVGAVADFDKDGKCQDLRIAVGAA
                     VETPQRVGGAEAMAHGQPLTDELVAAIAEQYAQTLDTLSDVRGSAWYRKEMVRVFVKR
                     ALEEVRDGHR"
     gene            complement(1214..1657)
                     /locus_tag="AC16V7_07330"
     CDS             complement(1214..1657)
                     /locus_tag="AC16V7_07330"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6256968.1"
                     /translation="MQIKDSFTIAAPQEKVWELFFDIPRLSQCVPGVESVEVLDDGTY
                     RGKLVVKVGPIKSQFGGIVTLTEVDPPHRIAGTLEGDDKANASSVKASFSSTLTSVVE
                     GTEATFVMDVNLRGRLAQFGGPVINATAKKLTAEFAKNLRAQLEH"
     gene            complement(1815..2147)
                     /locus_tag="AC16V7_07335"
     CDS             complement(1815..2147)
                     /locus_tag="AC16V7_07335"
                     /inference="COORDINATES: protein motif:HMM:NF014376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6256969.1"
                     /translation="MPTIFEPNDLPAKHGNGAKYITLANAAMLGVDALQVERVTLESG
                     KRAEPASAVDAEQFLYVIQGMGQAYVGSTTFQLAPESMLWIDPGDTYSLEAGSEGLEV
                     LICCAPAK"
     gene            complement(2159..3184)
                     /locus_tag="AC16V7_07340"
     CDS             complement(2159..3184)
                     /locus_tag="AC16V7_07340"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR6256970.1"
                     /translation="MKALVVKEFGGPLILEDLPVPQPAADEALIRVKACAVDQFDLTI
                     RDGKFAMANKVPIILGHEIAGVVEAVGTNVKTVAPGDRVTSTLYLTCGQCRYCLTGRE
                     TICADFKGYVGIHTPGAYAEYTTVPAINLVKMPETISFPDGSVLANAIGTPYHALTKR
                     ARLQAGERIIVTGAGGGVGTHAIQLARMMGAFVMAVDIGEKKLSLARELGAEAVFDAS
                     QGDFSSVAREWSNGEGVDVVLELVGTATFESSIKSLARGGRMVIVGSHTGSTLAASPQ
                     AMIANEWEILGSRNVSKRELVEVVNLVAAGRIRPVVTGTYPLEEAEAIHQRLRKQELI
                     GRVVLEP"
     gene            complement(3181..>3545)
                     /locus_tag="AC16V7_07345"
     CDS             complement(3181..>3545)
                     /locus_tag="AC16V7_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256971.1"
                     /translation="GTSVAFTVLTIISSIASTGMSPLVVSAGLLLFGLTAGYGFPAQL
                     NIRVNYFPVIRGTAGALQFFARFIGTTIAPILAGLLTDSFGLPSGYGLATAFLTLGAM
                     VAFFTVSNPVPAMEVVAE"
BASE COUNT          804 a         1030 c          954 g          757 t
ORIGIN      
        1 aacacagcag gcaattcgtc gtattcgata tcgagcagca tggcggcttc ctcggcagcc
       61 tcctcgctct cggcagcgat ggcagcgatc ggctctccca catagcgaac tgtatcaatt
      121 gccacgacgc cctggtcctt gatagacgcg ccgtaaaggg aatttggtcc caggtctgca
      181 cctgtcagga tggcgaccac gcccggcaca cgcagcgctt cggtcgtatc cactttcaac
      241 agattggcgt gtggcgattg gctgcgcacg atcttgccga tcagcatctc cggcaattga
      301 aggttgatca tgtagtccac agtaccggtt acgcgcgcca gggcgtctag catgggtatc
      361 gatttaccga tggccatctc gaacctcctc tagagcgcgc ttcacgaaaa cgcggaccat
      421 ctccttgcgg taccaggccg agccgcgcac gtccgataaa gtatcgagcg tttgggcata
      481 ttgctcagcg attgcggcga ccagttcatc ggttaggggt tgaccatgcg ccatggcttc
      541 ggcgcccccc acccgttggg gtgtctccac cgctgcgccg acagcaatcc gcagatcctg
      601 gcattttcca tccttatcga aatccgcaac tgcaccgacc gccacgcatg gccgcccctc
      661 ggctgagacg gacgtgtagc ggagataggc agcgcgggtt gtttcgggaa gggtggggat
      721 gacgacctcc gccaggattt cggtcggatt gagggcggtg gtgtagaaac caaggaagaa
      781 ttcagatagg ggaacgtccc gcgtcccttc taaactgatg agcctggcgc gcgcccccag
      841 agccgtcaac atggctgggg gatcggcggc gtaatctgca gccgacaggt taccacccag
      901 ggtggcttga ttgcgcacgc gcacgttacc cacaacgctg aaggtgtgcg ccagcgcagg
      961 gaaaaactgg cgcacgactg gcgaaagttc tgcatcgtgc aaggggatca gcgcgccaat
     1021 atgcaggcca tcgggctccg tgcggacgaa gtcccatccc gatatgcgcc cgaggctgat
     1081 aagcacgctg ggtgcgatca atttttgctg caacataagc accactgacg tgccgcccgc
     1141 aatcactttg gcatcgtctc cgtattcgga tagcagggcg acagcctctg aaaaatcaac
     1201 tggctccaga aaatcaatgc tcaagttgtg ccctcagatt ctttgcaaac tcagccgtca
     1261 attttttagc tgtagcgttg ataactggtc caccgaactg cgccagccgt ccgcgcaggt
     1321 taacatccat cacaaaagtg gcttcggttc cctccaccac gctggtcagt gtgctgctga
     1381 aactggcttt gacggaagac gcattcgctt tgtcatctcc ttcgagtgtg cctgcgatac
     1441 gatgcggcgg atcgacttct gtcaaggtga caatgccgcc gaactgcgat ttgattggcc
     1501 cgactttgac gacgagcttg ccgcgataag tcccgtcgtc caagacctct acggactcga
     1561 cgcctggtac acactggctg agacgaggga tgtcgaaaaa caattcccag actttttctt
     1621 gtggagcagc gatggtgaag gaatctttga tttgcatgaa tctctctgat taaaaacgcg
     1681 tcgtcctgag cggaggatcg aatagacagc gatcttttgc cgtatggatc cccccaatta
     1741 aagggcaatt ctcaggttaa tttctttgct tcgattatac ccatataaca gctcgttcat
     1801 ttcaatgata aatattattt cgcaggtgcg caacagatga gaacttccag tccctcggag
     1861 ccagcttcca gcgaataggt atctccagga tcgatccaca gcatggactc gggggcgagc
     1921 tgaaaggttg tgcttccgac atacgcctgt cccatgcctt gaatcacata caaaaattgt
     1981 tccgcatcca ctgcgctggc aggctcagcc cttttgccag attccaatgt aacacgctcc
     2041 acttgtaaag catccacacc caacatggca gcattggcaa gcgttatgta cttggctcca
     2101 tttccatgtt tcgctggtaa atcatttggt tcaaaaatgg tcggcataaa cactccaatc
     2161 aaggttccag gacaacacgt ccaatgagct cttgtttgcg cagacgctgg tgaattgctt
     2221 cggcctcttc aagcggatag gtaccagtca caacagggcg gatacgcccc gccgcgacca
     2281 ggttcaccac ttccaccaat tcccgcttgg acacattgcg cgagcccagg atttcccatt
     2341 cgttggcgat catcgcctgc gggctagcag ccagcgtgga acccgtatgc gacccgacga
     2401 tgaccatgcg tccgccacgc gccagcgatt tgatagatga ctcaaaagtg gccgtcccca
     2461 cgagttcaag aaccacgtcc acaccttcgc cgttcgacca ctccctggct acagacgaga
     2521 aatcgccttg cgaagcatcg aagaccgctt ccgcgcccag ttcgcgggcc agcgagagct
     2581 ttttctcacc gatgtccaca gccatgacaa aagcacccat catgcgcgcc agctggatgg
     2641 catgggttcc caccccaccg cctgcaccag tgacgatgat gcgttcacca gcttgcaagc
     2701 gagcgcgttt cgtcagagca tgataaggag tgccaattgc attggcaagc acgctgccat
     2761 cagggaaaga aattgtttct ggcatcttga ccaggttgat ggctggcacc gtcgtgtatt
     2821 cagcatacgc cccaggcgtg tggataccga cataaccttt gaaatcagcg cagatcgtct
     2881 cgcgtcccgt caggcaatag cggcattgac cacaggtcag gtagagggtg ctggtcacgc
     2941 ggtcgccagg cgcgaccgtc ttcacgtttg tgcctaccgc ctcgaccacg cctgcgattt
     3001 cgtgcccaag gatgattggg actttgttcg ccatagcaaa cttcccatcc cggatggtca
     3061 ggtcgaactg atccactgcg caggctttga cgcggatcag ggcctcgtct gcggcgggtt
     3121 gcgggacagg caagtcttcg agaattaagg gaccaccgaa ctctttgaca accagcgctt
     3181 tcattctgca acaacctcca tcgcggggac agggtttgaa accgtaaaga aggcgaccat
     3241 tgcgcccagc gtcaggaagg cagttgctag gccgtatccg ctaggcaggc caaaactgtc
     3301 tgtcagtaag ccagccagga tgggagcaat ggtcgtgcca atgaagcggg caaagaattg
     3361 caaagcgccc gccgtgccgc gtatgaccgg aaaataattc accctgatat tgagctgagc
     3421 aggaaatcca tagcctgcgg ttaacccaaa tagcaataat cctgccgata cgaccagtgg
     3481 cgacatgccg gttgaggcga ttgaactgat gatcgtgagg acggtaaatg caaccgaggt
     3541 cccca
//
LOCUS       DBNHOH010000415         4842 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1754281, whole genome shotgun sequence.
ACCESSION   DBNHOH010000415 DBNHOH010000000
VERSION     DBNHOH010000415.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1754281"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="AC16V7_07350"
     CDS             complement(<1..180)
                     /locus_tag="AC16V7_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256972.1"
                     /translation="MNTPSDRPRSLQTVIFILIAVGLIALALGGYLTPLSRILLNPMV
                     AVQTWVATRFQAVQDI"
     gene            complement(240..1310)
                     /gene="mreB"
                     /locus_tag="AC16V7_07355"
     CDS             complement(240..1310)
                     /gene="mreB"
                     /locus_tag="AC16V7_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HIR6256973.1"
                     /translation="MAFNPLNWLLGFFSLDIGIDLGTANTLVNVRGKGIVINEPSWVA
                     VEKRSRKPLAIGAEAKEMVGRTPANVIAIRPLRDGVISEFEITQAMLEYFIGKAHEQS
                     IVPVPRPRVVIGIPSGVTEVEKRAVYDASMSAGARETYMIEEPVAAALGAGLPVSEIR
                     GSMIVDIGGGTSEVAVMSMGGVVVSRSLRVAGDEMDEEIVQYMRNKYNLSIGLRMAEQ
                     AKISIGSAYHLPEEKTMLLRGRNLVSGLPESVEVSSVEVREAISGSVQTIIDTVKDAL
                     DEAPPEIIADLMDTGVCLAGGGALLQALDRRLTEDLRIRVWVAEDPMSCVARGAGMIL
                     EDLDHLNHLLVGLERGSTRHSQ"
     gene            1928..3769
                     /gene="glmS"
                     /locus_tag="AC16V7_07360"
     CDS             1928..3769
                     /gene="glmS"
                     /locus_tag="AC16V7_07360"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 -
                     L-glutamine:D-fructose-6-phosphate transaminase
                     (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR6256974.1"
                     /translation="MCGIVGYIGSRDATPIILNGLKRLEYRGYDSAGLAVLENGHIEV
                     RRDAGKLARLSAMVEDSPVHGNLGIGHTRWATHGEPSARNAHPHLGMTGNVVVVHNGI
                     VENFLELREELSAEGVEFKSDTDTEVIVHLVERYLSRDTGLAEAARSALSHLKGAHGV
                     VIFSSQEPDKIVAARVGNAGGVVVGVGEGEMFVASDMPAILEHTRRMVFLESRQMVVV
                     TRDGLEVKTLDGAPVNYEMHSVAWDPVAAEKGEYRHFMQKEIHEQVRSLTDTIGGRAD
                     FEEGRILLPELNLTPEFAQRLRKIFIIACGTAAHAGMVGKVLIEHIARIPVEVDIGSE
                     FRYRDPIIDQDTVVLAISQSGETADTLAAMEEARRKGATLWSIVNVIGSQAMRIADGY
                     VSMQTGPEIGVASTKAYTAPLVDLYMLAILLADLRGVISERERRRLVNDLRLVPDLAG
                     QCLDREQEVESVARALLHVNHCLYLGRGINMPTAYEGALKLKEISYIHAEGYPAGEMK
                     HGPIALIDKDMPVVVIAPRDPWYEKMLSQIEQAKARGGVVVALATDGDSLIPGLADYV
                     LWLPEAPWLLSPVTAVIPLQMLAYHIAALRGLDVDQPRNLAKSVTVE"
     gene            3835..>4842
                     /locus_tag="AC16V7_07365"
     CDS             3835..>4842
                     /locus_tag="AC16V7_07365"
                     /inference="COORDINATES: protein motif:HMM:NF016310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HIR6256975.1"
                     /translation="MSLQLSSSDQDMLAGAKGPAAKLAMSILARMAEVYGATELMDIS
                     AAHIDSTIYIGEAGLEFAERLAGLGAKVAVPTTLNVSGLDEHGWQEWAVPADWAAKAY
                     RQMVAYQSMGCVPTWTCAPYQTDLRPAFGQQIAWGESNAIAFANSVLGARTERYPDLL
                     DICCAITGRVPAVGLHLTENRAGQLLLRLVGVPLALQQDDSFYPVLGHLIGEVAGEQI
                     PVVDGLYARPTEDQLKALGAAAASSGAVALFHIIGVTPEAPTLGAAFQDRQPETVLSV
                     DMGWLRQARSELTTAQGDELQMIVLGSPHFSIQEFEQLSKLVAGQKAHPQVKFLVTSS
                     RV"
BASE COUNT         1074 a         1298 c         1403 g         1067 t
ORIGIN      
        1 aatatcctga acagcttgaa agcgcgtcgc gacccaggtt tggacagcga ccatgggatt
       61 gagtaagatg cgggaaagag gggtaaggta gccgcccagc gcaagagcaa tcaaccccac
      121 tgcaatcagg ataaagatca cggtttgcag tgagcgagga cgatctgatg gggtgttcat
      181 gttaaagcat tggtgattcc gtcaggtagg atatgccaga tagaatcgcc aggtattttc
      241 tattgactat gtcttgtgct tccacgttct aatccaacca ataaatgatt caggtggtct
      301 aagtcttcca agatcatccc agcaccgcgc gccacgcagc tcatggggtc ttctgcgacc
      361 cagaccctga tgcggagatc ctccgtcagg cggcggtcta gggcttgcaa tagagcacca
      421 ccacccgcca ggcagacacc ggtatccatc aggtcggcaa tgatctctgg cggagcctca
      481 tccaaggcat ctttcacagt atcgataatg gtctgcactg agccggagat cgcctcgcgg
      541 acttccacgg acgaaacctc taccgattca ggtaacccgc tcaccaggtt acgcccacgc
      601 agaagcattg tcttctcttc gggcaggtga tacgctgagc caattgatat cttggcctgt
      661 tcggccatgc gcaaaccaat gcttaaattg tacttgttgc gcatatactg aacaatctct
      721 tcatccattt cgtcgccagc cacgcgtaac gagcgggaga caaccacacc gcccatcgac
      781 atcacggcta cctcagaagt gccgccgcca atatctacaa tcatactacc acgaatttcg
      841 ctcaccggga gcccagcgcc cagagccgct gccactggtt cttcaatcat ataggtttct
      901 ctggctccgg cagacatcga agcgtcgtac actgcccgtt tctcgacttc cgtaacgccg
      961 gacgggatcc caatgaccac acgcggtcga gggactggca cgatgctttg ttcgtgggct
     1021 ttaccgataa aatattccag catggcttgg gtaatctcaa attcggagat cactccatca
     1081 cgcagtggtc ggatagcgat cacgttggct ggggtgcgtc ccaccatttc cttcgcctct
     1141 gcgccgatgg ctaacggttt tcggctgcgc ttttcgaccg ctacccaaga cggttcatta
     1201 ataacgatgc ctttaccccg gacattaact aatgtgttcg ctgtgccaag gtcgatgcca
     1261 atgtccagag agaaaaaccc caacagccag ttgaggggat tgaacgccaa ttcaggctcc
     1321 tccgtcgttc gctatggatg tgtatctctt taaggcattg ttaagaatgg ccctctcaga
     1381 cacatcggga tggattatac cagaacatga ccaccatcga atttcattat tcacattgtt
     1441 aaaattctca cattttccat taaaacatat cattttagtc taagaggcgt gtcgttttac
     1501 caatttccac accatttctg ttttgtacac aatgtcagac gatcgtgcgg gactatttag
     1561 cttacaatca tgaaaaccat gtcttaggga ttaggaatca ggtataattt tcgcacgtta
     1621 tgatctatat ttaggaggta agtggccttt ataagcaatt attctaggga gagatagcgc
     1681 aaggaccttc ttacagaagg ttgacgagga cgagggttcc cgcctgcgag ggcggggtaa
     1741 ccggctaccc tttaggttgc cagcctgagg gccgggaaaa tcgaatgatc agcggaagcc
     1801 ctccgggcca gccacggccc gctttctttc cctccagtgt aaacttcgcg gcaaatgctg
     1861 cccgggtaac cgcacagaca aaaccgtgaa gagtggcaga atctaaaaga aatttggagg
     1921 gaatcccatg tgtggaatcg ttggatacat tggatcgcgg gacgccacgc ccatcattct
     1981 gaatgggttg aaacgtttgg aataccgcgg ttatgactcc gctggactgg cggtgttgga
     2041 gaacgggcat atcgaggtgc gccgcgacgc aggcaagctg gcgcgccttt cggcgatggt
     2101 cgaggattcg cctgttcacg gaaacctcgg cattgggcac acacgctggg ctacgcatgg
     2161 cgagccaagt gcccgcaatg cccatcccca tttaggcatg accggcaacg tagtggtcgt
     2221 gcacaatggg attgtggaga atttcctgga gctgcgcgaa gagctcagtg ccgagggagt
     2281 agaatttaaa tcggatacag atacggaagt gatcgttcac ctggtcgagc gttatctcag
     2341 ccgagataca ggcctggcgg aggcagcccg cagcgcactc tctcacttga aaggcgcaca
     2401 cggtgtggtc attttttcat cacaggaacc tgacaagatt gtggccgctc gagttggtaa
     2461 tgcgggcggg gtggtggtag gtgtgggtga gggagagatg tttgtagcct cagatatgcc
     2521 cgccatcttg gagcacaccc gccgcatggt gttccttgag tcgcggcaga tggtggtggt
     2581 cactcgagac ggcttggagg tgaagacact ggacggcgcg ccggtgaact atgaaatgca
     2641 cagcgtagcc tgggatccag tggcggccga aaaaggcgaa taccgtcact tcatgcaaaa
     2701 agagatccac gagcaggtgc gttcgctcac ggataccatc ggtgggcgcg cagattttga
     2761 agaggggcgc attctgctgc ccgagctcaa cctgacccca gagttcgctc aacgtctgcg
     2821 taaaatcttc atcatcgcct gcggtacggc tgcccacgcc ggcatggtag gaaaagtgct
     2881 aatcgagcac atcgcccgaa taccggtgga ggttgatatt ggctccgagt tccgctatcg
     2941 cgacccaatt atcgaccagg atactgtggt gctggcgatc agtcagtcgg gcgagacggc
     3001 ggacacgctg gcagccatgg aggaggcgcg ccgtaaagga gctaccttgt ggtccatcgt
     3061 gaacgtcatc ggctctcaag cgatgcgcat agccgacggg tacgtctcta tgcaaaccgg
     3121 tcccgagatc ggtgtggctt ctaccaaggc ctatacggca cccttggtgg acctgtacat
     3181 gctggcgatc ttgctggcag acttgcgcgg tgtgatcagc gagcgtgagc gccggcgctt
     3241 ggtaaatgat ttgcgcctgg tgcccgattt ggctgggcaa tgcctggatc gcgagcagga
     3301 ggtggagagc gttgctcgcg ccttgctgca tgtcaaccat tgcctgtatc tgggccgggg
     3361 gatcaatatg cccactgcat acgaaggggc gctcaagctc aaagagattt cgtacattca
     3421 tgctgaggga tacccggcgg gcgagatgaa gcatggtcca atcgccttaa tcgataaaga
     3481 tatgccggtt gtggtgattg ccccgcgcga tccctggtac gagaaaatgt tgagccagat
     3541 tgagcaggcc aaggcgcgcg gcggggtggt ggttgctctg gcaacggatg gcgactcgtt
     3601 gatccctggt ttagctgact atgtgctgtg gttacccgag gcaccctggt tgctcagccc
     3661 ggtgacagct gtcatcccgc tgcaaatgtt ggcttatcac attgccgccc tgcgtggtct
     3721 ggacgtggac caacccagga atctggcgaa aagcgtcacc gtggaataaa agttcttaac
     3781 cggcgatcaa ggcgtatcct ggtacgccgc ttgtctttca gaaaattggg attaatgtct
     3841 ctgcagcttt cttcatccga tcaggatatg ctggcaggtg ccaaaggccc ggcagccaag
     3901 cttgccatgt ccatcctggc gcgcatggct gaggtgtacg gcgcgaccga gctgatggat
     3961 atcagcgcag cccatatcga cagcacgatc tatatcggcg aggccgggct ggagtttgca
     4021 gagcggctgg ccggactcgg cgctaaggtc gcagtgccaa ccacattgaa cgtgagtggc
     4081 ctggatgagc atggctggca ggagtgggct gtgccggccg attgggcggc gaaagcctac
     4141 cggcaaatgg tcgcatacca aagtatgggc tgtgtcccga cctggacctg cgccccatac
     4201 cagaccgatt tacgcccagc attcggtcag cagatcgcct ggggcgaatc aaacgctatc
     4261 gcttttgcca attcggtcct gggggcgcgc accgaacgct accccgatct gctggatatc
     4321 tgctgcgcca tcaccgggcg tgtcccggcg gtagggctgc atctgacgga gaaccgggct
     4381 ggtcagctgc ttttacgcct ggtgggcgtt ccgctagcgc tccaacaaga cgacagcttc
     4441 tacccggtgc tgggtcactt aattggtgaa gtggctggtg agcaaatacc ggtggtggat
     4501 ggtttgtatg ccagaccaac cgaagaccag ctcaaagccc tcggcgcagc ggcagcttca
     4561 tcgggggcgg tggcattgtt tcacatcatc ggtgtcaccc ccgaagcccc cacgctgggt
     4621 gcggcattcc aggataggca accggagact gtgctctctg tggacatggg atggctgcgc
     4681 caggccagga gtgagctgac caccgcacaa ggcgacgagc tccaaatgat cgtgctgggc
     4741 agcccgcact tctccatcca ggagtttgag cagctatcca agttggtggc tgggcagaag
     4801 gcacacccgc aggttaaatt cctggtcacc tctagccgtg tg
//
LOCUS       DBNHOH010000416         1754 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_664791, whole genome shotgun sequence.
ACCESSION   DBNHOH010000416 DBNHOH010000000
VERSION     DBNHOH010000416.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_664791"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..234)
                     /locus_tag="AC16V7_07370"
     CDS             complement(1..234)
                     /locus_tag="AC16V7_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256976.1"
                     /translation="MKQKVIQSDEMAVEPRVLHQAQREALPGGLTNRFLLGSCRRRGW
                     RAGNFLYLIQQALAEGIAHVENVWLLCGEVEKS"
     gene            352..1203
                     /locus_tag="AC16V7_07375"
     CDS             352..1203
                     /locus_tag="AC16V7_07375"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6256977.1"
                     /translation="MDKKFYTLIQCDLCGHDLELTDDALGCTNCGRSVPLQDGKPIFS
                     SPPPGLQVSEKIVRGPEIGTPWRQANWRFLAEQVAALEPEAVILDVGAGRGDFADLFE
                     GRNYLALDVYPYPEVDIVCDLTRSNPFRAASLDTILLMNVLEHVYDTHALLASLAEML
                     KGDGTLLIAIPFLVKMHQEPVDFVRYTHYALERMGAEHGLALEHLEGFYDPISLLGEG
                     TGNIKHAVLPNLRGFRHYAGRLLLLGMEALSAGMRWAVGEEQMRHPQEARSHAPTGYH
                     VIYRKKY"
     gene            1205..>1754
                     /locus_tag="AC16V7_07380"
     CDS             1205..>1754
                     /locus_tag="AC16V7_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256978.1"
                     /translation="MGTKQTVFICADHGLAVFYFLQSDIIPVLLEAGINVVLLTDDAT
                     TELVAERYARPSMIVEGLRLDQIQQYTRNVSPTLQWWLDFLRRAGAAAGTNLAVVDAY
                     IQQVKSEAHARRRQLFPLMEAVARFMRRSRPARSLLSNLQRRFTPQIYADLFEKYHPD
                     LVIAGSPGFRQDRYLLREAAARE"
BASE COUNT          374 a          531 c          479 g          370 t
ORIGIN      
        1 ctaggatttc tccacctctc cacacagcag ccatacgttt tccacatggg caataccctc
       61 agcgagcgcc tgttggatga ggtaaagaaa gttaccggcg cgccagccgc gcctgcggca
      121 ggagccaagc aagaaacggt tcgtaaggcc gcctggcagc gcctcgcgct gcgcctggtg
      181 caacaccctc ggttcaaccg ccatttcgtc agattgtata actttctgtt tcaagcttta
      241 cactccaaaa gatgacgagt ctcgatcacc ggtcacctcg cttggaggct gagacaagaa
      301 ccacaatgct caaaatccgt gctgatctgt gtgtatgtgt ggtttctcgc aatggataag
      361 aagttttaca ccctcatcca gtgcgatctc tgcggtcatg atctggaact cacggatgac
      421 gcgctgggtt gcacaaactg cggcaggagc gtaccgctgc aagatggtaa gcccatcttt
      481 tcatctccgc cgcccggctt gcaggtctcc gagaagattg tccgtggccc agagatcggc
      541 acaccctggc ggcaagccaa ctggcgcttt ttagcagagc aggttgctgc acttgaacca
      601 gaagcggtca tcttggatgt gggtgcaggg cggggcgatt ttgctgactt attcgagggg
      661 cgaaattacc tggcattgga cgtctaccca taccccgaag tggacatcgt ctgtgacctg
      721 acacgcagca atccctttcg ggcggccagc ctggatacca tactgctgat gaacgtgctg
      781 gagcatgttt acgacacgca cgccttgcta gcctcgctgg cagagatgct caaaggggat
      841 ggcaccctgc tgatcgccat ccccttcctg gtcaaaatgc accaagagcc ggtggacttc
      901 gtgcgctata cccactacgc cctggagcgc atgggcgccg aacacgggct ggcgctggag
      961 catctggagg gcttttacga cccgatctca ctccttggcg aaggcaccgg caacatcaag
     1021 cacgccgtgt tgcccaacct gcgcggattt cgccattacg ccgggcgctt gctgctattg
     1081 ggcatggaag ctctttccgc tggcatgaga tgggcggtcg gtgaagagca gatgagacat
     1141 ccacaggaag ctcgctccca cgcgccgacg ggctatcacg tcatatatcg aaaaaaatat
     1201 taatatggga acgaaacaaa ccgtctttat ctgcgccgat cacggtctgg ctgtttttta
     1261 ctttcttcaa agcgatatca tccccgtcct actcgaggcg ggaataaatg tagtgctgct
     1321 gactgacgac gccaccactg agctggttgc cgaacgctat gctcgcccga gcatgattgt
     1381 cgaagggctg cgcctggatc aaatacagca atacacccgc aatgtctctc ccacgctcca
     1441 atggtggctg gatttcctgc gccgggctgg cgcagccgca ggcaccaacc tggctgtggt
     1501 agatgcctac atccagcagg tcaagagcga ggcacatgcc cgccgcaggc aattattccc
     1561 actgatggaa gccgtcgccc gcttcatgcg ccgctcacga ccagctcgca gcttgctctc
     1621 caacttgcag cggcgtttca cgccccagat ctacgccgac ctgttcgaga aataccatcc
     1681 agacctggtg atcgccggct cgcccggctt ccgtcaagac cgctacctgc tgcgcgaagc
     1741 ggcggctcgg gaga
//
LOCUS       DBNHOH010000417         2795 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1754360, whole genome shotgun sequence.
ACCESSION   DBNHOH010000417 DBNHOH010000000
VERSION     DBNHOH010000417.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1754360"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="AC16V7_07385"
     CDS             <1..193
                     /locus_tag="AC16V7_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6256979.1"
                     /translation="SWLFRTNYAQPVPFVNHSKNIPAIQTPIPGLYFASMSQVYPWDR
                     GTNFAVEIGRRAAGIMLGE"
     gene            228..1412
                     /locus_tag="AC16V7_07390"
     CDS             228..1412
                     /locus_tag="AC16V7_07390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037043.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP-like domain-containing protein"
                     /protein_id="HIR6256980.1"
                     /translation="MDLATFRNLLTPLGQEVLQAATELAPVEEDFLRHFTSLSRHYPV
                     ELARLALGIAILRKQGERKFPFANQLYFTREALEQASSWEVSSYRAQRYRGFSQLIDL
                     GCSVGSDTLALAQIAPTIGLDLDPLRLAMAQANLAALGLSASSLIQADLQSPLPISHT
                     TSSPALFFDPARRARHRRAFSVRDYQPPLAIIKEWLPRFPALGVKISPGVNLNELTPY
                     DAEIEFISLNGDLKEAVLWFGPLQSAYRRATLLPGGHTLFTDTSLEMRQSIDEPRAFL
                     YEPDPAVLRAGLVVMLGAQLNAAQLDPDIAYLTAHEYKATPFAKAWEVEAWFPFGLKR
                     LRAYLRQRHVGRVTVKKRGSPLEPETLISELRLSGDEERTIFLTHLRGKPIVVIGWKV
                     ED"
     gene            1560..1877
                     /locus_tag="AC16V7_07395"
     CDS             1560..1877
                     /locus_tag="AC16V7_07395"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR6256981.1"
                     /translation="MPQKIVDPIVFAKALADETRQKIMSLCCCQWLSVGEIVDALDVS
                     QPTVSHHLSLLRSAGLVSVRREGKQVFYTLNQSRMAEGCCTLAEAFAPDHELNLSPRE
                     QNN"
     gene            2158..>2795
                     /gene="arsM"
                     /locus_tag="AC16V7_07400"
     CDS             2158..>2795
                     /gene="arsM"
                     /locus_tag="AC16V7_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007999365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HIR6256982.1"
                     /translation="MTIEQNTQDIRTFVRERYGKIAEDFQPTSEASCGCAPSDSDCCG
                     GESSSSVRFYDTPDAAALPDEVTGLSLGCGDPVTLAALQPGQTVLDLGSGGGIDCFLA
                     AQRVGADGQVIGIDMTPAMLDKARANQKKLGIENVEFRLGEIEHLPVPDSTVDVIISN
                     CVVNLSPDKPQVFREAFRALKSGGKLAVSDLVTDGPLPQAVKSSLSAWGGCVA"
BASE COUNT          628 a          777 c          758 g          632 t
ORIGIN      
        1 atcctggctt ttccgcacca actatgccca gcctgtgccc tttgtgaacc actcgaaaaa
       61 catccccgca attcagacac ccatcccagg actttacttc gccagcatga gccaggtata
      121 cccgtgggat cgggggacca acttcgccgt agagataggg cgcagggcag ccggaatcat
      181 gctcggcgaa taggcaatgg gagcatgttg cctgataaaa tgcaggtgtg gatttagcta
      241 cttttcgcaa tttgcttacc ccactcgggc aggaagtact gcaagctgct accgaattag
      301 cgcccgttga agaggatttt ttacggcatt tcacctccct aagccggcat tacccagtgg
      361 agctggctcg cctagcctta ggtatagcca tcctgcgcaa gcagggggag cgcaaattcc
      421 cgttcgcaaa ccagctttac tttacacgcg aagccctgga gcaagcctcg agctgggagg
      481 tttcgagcta ccgcgcccag cgataccggg gattctctca attgatcgat ttaggctgct
      541 cggtgggcag tgacacgctg gcgttggcgc aaatcgcccc cacaattggg ctggacctcg
      601 accccttgcg cctggcaatg gcccaggcaa acctggctgc cttgggactc agcgcatcat
      661 cactcatcca agcagacctg cagtcccctc tccccatatc tcacacaact tcctcgcctg
      721 cccttttctt cgatcccgcc cgccgagcaa gacacaggcg tgcattttct gtacgcgatt
      781 accagcctcc gctggcaatc atcaaagagt ggctgccccg tttcccggcg ctgggtgtga
      841 aaatctcccc gggggtgaat ctgaacgagt tgacacccta tgatgccgag atcgagttta
      901 tctccttgaa cggggatttg aaggaggccg tactttggtt tggaccgctc caaagcgcct
      961 atcgccgggc aacccttctc cccgggggac ataccctgtt caccgacaca agcctggaga
     1021 tgaggcagtc catcgacgag ccgcgcgctt tcctgtacga gcctgatccg gcagtgctgc
     1081 gtgctggtct ggtggtgatg ttaggagccc agctcaacgc ggctcagctc gatccagata
     1141 tcgcctacct gaccgcccac gagtacaaag ccaccccctt tgccaaagcc tgggaggtgg
     1201 aggcatggtt cccatttggg ttgaaacgtc tgcgagccta tctccgccag cgccatgttg
     1261 gacgggtgac ggtgaaaaag cgcggctcgc cgcttgagcc ggaaactttg ataagcgaat
     1321 tgagactatc cggtgacgaa gagcggacga ttttcctcac ccacctgcgg ggcaaaccta
     1381 tcgtggttat tggttggaag gtggaggatt aagaacgggt aggaatgcct ggatgcatta
     1441 ggtaggtctg tttgctgctt gacatataga tgaacttcta ttagtatatg gtcaatcgat
     1501 ttactttcga gaagctactc tatcaattta ttgttataac tggaatgaat gggtaaacca
     1561 tgccacaaaa aattgttgac ccgattgtat ttgctaaagc cctggcggat gagacccgcc
     1621 agaagatcat gagcctgtgc tgctgccaat ggctcagcgt aggagagatt gttgacgcct
     1681 tagacgtatc tcagccgacg gtgtcgcacc atctgagcct gctgcgctca gccggcctgg
     1741 tgagcgtgcg tcgcgaaggc aagcaggtgt tttacacgct taaccagtcg cgcatggccg
     1801 aaggatgttg cacgctggca gaggcttttg ccccggatca cgagctgaat ttatccccac
     1861 gcgagcaaaa caactaaaaa gttcttgata cctgaatctc cttatgagga gaaatccaga
     1921 ttcagcgcca gggcaattgc atatgcccct cagggcatgc gcaatcccgc aaaaagagtg
     1981 tttgaggtgt tttggtgggg gtatgcccca ccaaaacacc ccattttctc tcctcagtgg
     2041 tgggaaatcg cccaaatgtg attgccctaa ttcagcgcat ttaatcttga caagagtaaa
     2101 aactgcgtgt aacatataga cacatataaa tatttctata tatatcagga gaatataatg
     2161 accattgaac aaaacaccca ggacattcgc accttcgtgc gagaacgata tggaaagatc
     2221 gctgaggatt tccagccgac ctcagaagcc tcttgcggct gcgccccatc ggatagcgat
     2281 tgctgtggcg gagagtccag tagctctgtg cgcttctacg acacccccga cgcagctgcc
     2341 ctgccggatg aagttaccgg tctctctttg ggttgcggcg atccagtgac gctggccgca
     2401 ctacagcctg gtcaaactgt attggacctg ggctccggtg gtggcatcga ttgcttcctg
     2461 gctgcgcagc gggtgggcgc tgatggccag gtaattggca tcgatatgac tcccgccatg
     2521 ctggataagg cgcgcgccaa ccaaaaaaag ctgggcattg agaatgtaga attccgattg
     2581 ggcgagatcg agcacctgcc tgtaccggat agcaccgttg acgtgatcat ctcaaactgt
     2641 gtggtcaatc tcagtcccga caagccccag gtctttcgcg aggcttttcg tgcgctcaag
     2701 tcgggcggca agcttgccgt gagcgatcta gtgacggatg gtcccctgcc ccaggcagtt
     2761 aagtccagcc tcagcgcctg gggaggctgc gtggc
//
LOCUS       DBNHOH010000418         3604 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1497841, whole genome shotgun sequence.
ACCESSION   DBNHOH010000418 DBNHOH010000000
VERSION     DBNHOH010000418.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1497841"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..1099)
                     /locus_tag="AC16V7_07405"
     CDS             complement(155..1099)
                     /locus_tag="AC16V7_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003343087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6256983.1"
                     /translation="MQATASSTDVPIIAERSPSRIRTTIRILLRNRSAVFGIVILLSM
                     TLIAIFAPLLSTHDPLKPLIGIEQVAKRAPPCIHLLGCPQDQPQHFFGTDGNVRDEFS
                     RVLYGARLSLIIGFTTVTFAIIIGTILGLVSGYAGGWVDNVIMRSMDVLLAFPSLLLA
                     IAIVTVLGPGLINALLAIGIVSIPTFARVTRASVLLLKEMDFVSASRALGADSVTVVL
                     KHILPNSLTPLIVQGTLGIATAILDAAALSFLGLGAQHPTPEWGLMVGQEYKNLFNAP
                     HLTFFPGIALMLVVLSFNLLGDGLRDALDPRLSHTRTG"
     gene            complement(1102..2178)
                     /locus_tag="AC16V7_07410"
     CDS             complement(1102..2178)
                     /locus_tag="AC16V7_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6256984.1"
                     /translation="MTQYIARRLLSSLPVFFGILFATFALARLLPGDPCRAVLGERAT
                     DQVCDAFIKRMGLDRPIPVQFGIYMRDVFQGDLGVSLRQGRPATEILAERLPTTIELA
                     FYAMLFATIVGVPLGIISAYWRNSPIDLSTMILANVGVSTPVFVLGLLLEYFFAIILK
                     DTFLWLPPSGRISSGFSLPSLAETWNLTDLSPFWLAVVEFISRLYTFNALVTRNWEVL
                     ADVWSHMILPSVAVGTIPLAIVARMTRSSILDVLGLDYIRTARAKGLRMPQVVFRHAL
                     RNALLPVVTIIGLSLGGLLSGAVLTETVFSLPGVGRTLFESITARDYTVVQAFTLVIA
                     IVYVVINLIVDVLYAFLDPRIQLS"
     gene            complement(2256..>3604)
                     /locus_tag="AC16V7_07415"
     CDS             complement(2256..>3604)
                     /locus_tag="AC16V7_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6256985.1"
                     /translation="VGTGPFVLEAWNRGESVVLKRFEDYWGDPAKAPTAELKWSQEGA
                     QKLIELQAGTVDGIDNVAPDDIPNVQADATLQLTPRAAFNIFYVGMNNTYAPYDKEEV
                     RQAIAMAIDRKRIVDTFYPPGSAVADQFLPEGLLARADSQWYEFDVAKAKELLTAAGF
                     PDGFKTVLNYRNVFRGYLPEPPVVAQDIAAQLKENLNIDVEIVEIESGTFIDMSDNGE
                     LEGLHLLGWTGDYPDPTNFYDFHFGTGATDQFGTKWDDITSRLSQAAQLGDEEARRVI
                     YAEVNDLLKQHVPMIPIAHGASAAAYKADVQGAHASPLGNESLEVMYIEGQDTFTFMQ
                     NAEPIGLYCADESDGESLRACEQIGESLLAYEVGGVEVIPSLATGYTSNDDATEWVFN
                     LREGVLFHDGSTLDANDVVLSYVVQWDAAHPLHVGRDGNFSYFSGLFGGFLNPPPP"
BASE COUNT          940 a          947 c          998 g          719 t
ORIGIN      
        1 gttctcgggg aggggtgaat gggagaaata caatatacat gattaacccc gaatttaatc
       61 tttccgggat taatcgtata cttcaacccc cacctgccgt tcgggcaggg aaggagaatg
      121 attacaaacc aaatacaaaa ttagctgagc ctgttcaacc tgtgcgcgta tgagacaggc
      181 gcggatccag cgcgtcgcgc agaccatccc ccagaaggtt gaaggacaaa accaccagca
      241 ttaaagcaat gcccgggaaa aaggtcaagt gcggggcgtt gaagaggttt ttgtattctt
      301 gccctaccat caatccccat tcaggggtgg gatgttgggc gcccaaaccg aggaacgata
      361 atgccgccgc gtcgagaatg gccgtggcga tgcctaacgt accttgcaca atcagcggag
      421 tgagcgagtt gggcaagata tgcttcaata ccaccgtgac gctatccgcg cccagggcgc
      481 gcgaggcact gacgaagtcc atttctttca gcaggagaac gctggcgcgc gttacccgcg
      541 cgaatgtggg gatcgagaca atgccaatcg ccagcaaggc gttaatcagc ccaggcccca
      601 aaacggtcac aatagcaatc gccagcagca gggagggaaa tgccaggagc acatccatac
      661 tgcgcatgat gacattatcc acccagccgc cagcataacc gctgaccaac cccaggatgg
      721 tcccaataat aatggcgaag gtgaccgtgg tgaaaccaat gattaaggac agtcttgcgc
      781 catatagcac gcggctgaat tcatcgcgca cattgccatc agtgccaaaa aaatgctggg
      841 gttgatcttg cgggcaaccc aataaatgga tgcacggcgg agcgcgtttt gctacctgct
      901 caataccgat taatggtttc aacggatcgt gggttgacag gagcggcgca aagatagcaa
      961 ttaaagtcat gctaagcagg atgacgatac caaatacagc tgaccgattg cgcagcaaga
     1021 tacggatagt tgttctgatc cggctaggtg atctttcagc gataataggc acgtcagttg
     1081 aagatgctgt agcctgcata gtcagctcag ttggatgcgt ggatcgagaa aggcgtagag
     1141 tacatcaaca ataagattaa taacaacata aactatagcg atcaccaggg taaatgcctg
     1201 caccacagtg taatcgcgcg cggtgatgga ttcaaaaagt gttctaccca cgcctggaag
     1261 gctgaaaacc gtctcagtga gcactgcccc gctgagtaac ccgcctaacg agagaccaat
     1321 aatggtcacc acggggagga gggcattgcg caaggcgtga cgaaatacaa cctggggcat
     1381 cctcaaccct ttggcgcgcg cggtgcggat ataatccagc ccgaggacat ccaggatact
     1441 tgagcgcgtc atgcgagcga caattgccaa gggaatcgtg cccactgcca cagacggcag
     1501 gatcatatgg ctccaaacat ctgcgagcac ttcccagttt ctagtcacca aggcgttgaa
     1561 cgtgtacaag cgggagatga actccacaac ggcaagccag aatggtgaca gatccgtcag
     1621 attccaggtc tccgccagag agggtagcga gaagccggaa gatatccgtc cagatggggg
     1681 cagccagaga aaagtatctt taagaattat tgcaaaaaag tattccagca gcaaacccag
     1741 gacgaaaact ggcgtggata cccccacgtt cgccaaaatc atggtgctca gatcgatcgg
     1801 cgagttccgc cagtaggccg agataatacc caatggcaca cctacgatcg tggcgaacag
     1861 catggcatag aaggcaagct cgatggtggt tgggagccgc tcagcgagga tttcagtcgc
     1921 gggtctgcct tgacgcaagg aaacacccag atcgccttga aagacatctc gcatatagat
     1981 gccaaattgc acggggatcg gacgatccag gcccatgcgt ttgatgaagg cgtcgcacac
     2041 ttgatcggtg gcgcgctcac ccagcactgc ccggcacgga tcgccaggca gcaatctcgc
     2101 cagagcgaaa gtggcgaata atatgccaaa aaataccgga agcgaggata acaaacgtcg
     2161 ggcaatgtac tgcgtcatgg cataatcaat acatgatttt gatggggcat tgcaagaagc
     2221 aatgccccat cgatgactat taacttaatc gtagtttatg gtgggggcgg gttcaggaag
     2281 ccgccgaaca agccggagaa gtaggagaaa ttaccatctc gaccgacatg cagcgggtga
     2341 gccgcgtccc actggacgac ataagacagg accacatcgt tggcatccag cgtggagccg
     2401 tcgtggaaca gtactccctc gcggagattg aacacccact cagtggcatc atcattcgat
     2461 gtgtaaccag ttgccagcga agggatcacc tctacgccac ccacttcata ggcaagcaac
     2521 gactcgccaa tctgctcaca ggcacgcagg gattcaccat cagactcatc ggcgcagtac
     2581 aggccaatcg gctcggcatt ctgcatgaag gtgaaggtgt cttgtccctc aatgtacata
     2641 acctccaggc tttcgttgcc cagcgggctg gcgtgagcac cctggacatc agccttgtag
     2701 gcagccgcag aagcaccatg ggcgatgggg atcatgggta catgctgctt gagcaggtcg
     2761 ttgacctcgg cgtagatcac ccggcgggcc tcctcgtcac ctaattgggc agcttgactg
     2821 agtcgtgagg tgatgtcatc ccacttggtt ccaaactggt cggttgcgcc agttccaaag
     2881 tggaaatcat agaagttggt cggatcagga tagtcgcccg tccagcctaa cagatgcagg
     2941 ccttctagct cgccgttatc ggacatatcg ataaaggtgc ccgattcgat ctcaacaatc
     3001 tctacgtcga tattgagatt ttccttcaat tgggcagcaa tatcctgggc gacaactggc
     3061 ggttctggca ggtagccgcg gaagacattg cggtagttaa gcacagtttt gaacccatcc
     3121 gggaaaccag ccgcggtgag caattccttg gctttggcga cgtcgaactc gtaccattgt
     3181 gaatcggcgc gagccagcag gccttccggc aggaattgat cagcaacagc tgacccaggc
     3241 gggtagaagg tatccacgat gcgcttgcgg tcaatcgcca tggcgatggc ctggcgaact
     3301 tcttctttgt catagggtgc gtaagtattg ttcatcccga catagaaaat attgaacgcc
     3361 gcacgaggtg tcagttgcag agtggcatca gcctgaacgt tggggatgtc atccggcgca
     3421 acgttatcga tgccatcgac tgtgcctgcc tgcagctcga tcaacttctg ggcgccttcc
     3481 tggctccatt tgagttcggc ggttggagct ttggcagggt cgccccagta atcttcgaag
     3541 cgcttgagca caacactctc gccgcgattc caggcctcga gtacgaaggg tcctgtacca
     3601 accg
//
LOCUS       DBNHOH010000419         2546 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_985762, whole genome shotgun sequence.
ACCESSION   DBNHOH010000419 DBNHOH010000000
VERSION     DBNHOH010000419.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_985762"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="AC16V7_07420"
     CDS             complement(<1..732)
                     /locus_tag="AC16V7_07420"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HIR6256986.1"
                     /translation="MLTNIWWIRRDLRLADNLALNEARKGAGALVPLFILDPHLLKQP
                     APSRKAFLFEGLRRLDEDLQRMGNRLVVRSGDPLDVLRRVVAESGAQRIYAVEDYSPY
                     ALQRDEHIGRVLPLTLQPGVTVRHPASVAKPDGSPYTVYTPFSKAWKALTLTAGNSTL
                     ETLPAPEYLPGSIPLDSESLPEDDPPELFPAGEAEARRRLEHFCEDLIYAYTEDRNRL
                     DLEGTSALSPYLRFGMLSPRQALNAA"
     gene            1009..2412
                     /locus_tag="AC16V7_07425"
     CDS             1009..2412
                     /locus_tag="AC16V7_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR6256987.1"
                     /translation="MPQLMRCLYCGLLQDEPAGVKSCARCGGELGYESMPTSDERSSY
                     VSVQMELDQITAPSGQNCDRYLMVTLRTPAQVPAEQAAPTQSGRPPLNFCAILDVSGS
                     MQGEKITQAKEAVRQTLNHLREGDVFSLVTFSSDVRAVFEPRLVDSQTRRVVESALQE
                     IQAGGMTALDGGLALGLEKIASRKEETNLALLLSDGQANVGETDLEKVGQRGYAARQQ
                     GVIVSTLGVGLDYNEALMVEIATQGGGRFYHLHSAGQIAVFLAGELGEVTSLAARETT
                     LQLDLPPGATLVPLSSAYLAQQSGGQASVVIGDIPSDTELEIPLRLTLPAQSPGTKLS
                     IEGSLQYRSPAGNLLHTKLNRVTVRFVKKAIFKLRDGIVLPVAERVFEQMKAASVLRV
                     SRAYAMNAVDGQRQSEERLAAMHEYAVKLGDERAVEEMKIAEQSFGFMASSPSAAKAA
                     VADAFSKQRSSKDFDKK"
BASE COUNT          585 a          729 c          719 g          513 t
ORIGIN      
        1 ggctgcattc agcgcttgcc ggggagagag catgccaaaa cgcagatagg gggagagagc
       61 cgaagtgcct tccaggtcca ggcgattgcg atcctcggta tacgcataga tcaggtcttc
      121 acaaaaatgc tccagacggc gccgcgcttc tgcctcacca gctgggaaca gctcgggcgg
      181 atcatcttca ggcagtgact ccgagtccaa cgggatcgaa ccaggtaaat attctggggc
      241 aggcagcgtt tccaacgtcg aatttcctgc ggtcaacgtc aaggctttcc aggccttgct
      301 aaatggggta tacaccgtat aggggctgcc atctggcttt gccactgagg cgggatggcg
      361 aaccgtcacg cccggctgca aagtcaacgg caaaacccgg ccaatgtgct catcgcgttg
      421 gagggcataa gggctgtaat cttccaccgc gtatatgcgc tgtgcaccgc tctccgccac
      481 cacccgccgc agtacgtcca aggggtcgcc agagcgcacc accaggcgat tgcccatgcg
      541 ctgcaaatcc tcatccaaac ggcgcaaccc ttcgaagaga aacgccttac gcgatggtgc
      601 cggctgcttg agcaaatgcg gatcaagaat aaatagcggg actagcgctc ccgctccttt
      661 tcgtgcctcg ttcaaagcca gattgtccgc caggcgcagg tcacggcgga tccaccagat
      721 gtttgtaagc atgtctgcga acctctactt ctcgatttct aaagattata gctgataggc
      781 tttatcgtat ccagcttgag caagtattga tccaacgtaa taacactaat acgatagccc
      841 taggtttaga ttcacctctt gccgaattgc aagaacgttc tataatcatt agaagttacg
      901 cagttggata cgtggcaggg gggtttgggc ggatcatgac ccgcccaaac ccccctcctt
      961 tcctcttcaa ctgcgtaagt cctaatcata taaacaaagg aggaactcat gccacaactc
     1021 atgcgctgcc tgtattgtgg gctcctgcag gatgagcctg ccggtgtgaa atcctgcgct
     1081 cgatgcggcg gggagctggg gtacgaatca atgccaactt cggacgaacg aagctcctat
     1141 gtttccgtcc agatggagct agaccagatc accgcgcctt ccggacaaaa ttgcgaccgc
     1201 tatctaatgg taacactgcg cacgcccgca caggtgcccg cggagcaggc cgcgcctact
     1261 cagagcgggc ggcccccctt aaacttctgc gccatactgg acgtctccgg ctcaatgcaa
     1321 ggcgagaaga tcacccaggc caaggaagcc gtgcgccaaa cattgaatca cttgcgtgaa
     1381 ggagatgtgt tctcgctggt aactttttca tccgacgtgc gtgccgtttt cgagccacgc
     1441 ctggtagatt cccagacccg gcgtgtggtg gaaagcgctc tgcaggagat tcaagctggc
     1501 ggcatgaccg cactggatgg tggattagca ctcggactag agaaaatcgc ttctcgaaaa
     1561 gaggagacca acctggcatt gctgctgagc gacggacagg ccaatgttgg tgaaacggac
     1621 ttagaaaagg tcgggcagcg aggctatgca gcccggcaac agggcgttat cgtatccacg
     1681 ctgggggtgg ggctagacta caacgaggca ttgatggttg aaatcgccac tcagggaggc
     1741 gggcgttttt accacctgca ttccgctgga cagatcgccg tcttcctggc aggtgagctg
     1801 ggcgaggtga cttccctggc tgcccgcgag acaaccctgc aattagattt accccctggt
     1861 gccactctgg tgccgctttc gtcggcctac cttgctcagc aaagtggagg gcaagcatct
     1921 gtcgtcatcg gtgatatccc cagcgacacc gaattagaga ttcccctgcg cctgaccctg
     1981 cccgcccaat cccctgggac caagctcagc atcgaaggct cactccaata ccgctcccct
     2041 gcgggcaacc tgctccacac caagctgaac cgggtgacgg ttcgcttcgt gaaaaaggct
     2101 atcttcaaat tgcgagatgg catcgtgctg cctgtggctg agcgggtctt tgagcagatg
     2161 aaggccgcca gtgtgctgcg ggtctcccgc gcctacgcca tgaatgctgt ggatggccag
     2221 cgacaatccg aagaacgatt ggcggcaatg cacgagtatg ctgtcaagct gggagacgag
     2281 cgggcagttg aagagatgaa gatagcagag caatctttcg gttttatggc gagcagcccg
     2341 agcgcagcca aagctgcggt ggcggatgcc ttctcaaaac agcgcagctc gaaggatttt
     2401 gataaaaagt aaatcgctaa taaacatgaa cccccaccca cccctaattc ccttcgggct
     2461 acgcccgcag ggcgatgccg ccatccccca ctgggagggc ggggaatata tcggaaaaac
     2521 gggctattgg gcggcggaac cgccgc
//
LOCUS       DBNHOH010000420         2077 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1562084, whole genome shotgun sequence.
ACCESSION   DBNHOH010000420 DBNHOH010000000
VERSION     DBNHOH010000420.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1562084"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="AC16V7_07430"
     CDS             complement(<1..167)
                     /locus_tag="AC16V7_07430"
                     /inference="COORDINATES: protein motif:HMM:NF013629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HIR6256988.1"
                     /translation="MTHCDTLLQALRDLGYRITPQREMIVEAIAHASGHATAEEIFEQ
                     VKTRTKYVNLAT"
     gene            complement(358..681)
                     /gene="trxA"
                     /locus_tag="AC16V7_07435"
     CDS             complement(358..681)
                     /gene="trxA"
                     /locus_tag="AC16V7_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR6256989.1"
                     /translation="MSDLAHVNEADFQQEVLEASQPVLVDFTAVWCAPCKMLDPVVKQ
                     LAQEWAGKVKVVKLDVDSNPNLAMHYQVMSVPTLMLFVNGQPSQRIFGYQPKDRIIAK
                     FGPSI"
     gene            1139..>2077
                     /gene="pheS"
                     /locus_tag="AC16V7_07440"
     CDS             1139..>2077
                     /gene="pheS"
                     /locus_tag="AC16V7_07440"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIR6256990.1"
                     /translation="MLQKLEEIQRAALDALEAVDDEGALENWRVAYLGRSAPLMSVFD
                     QLGSLPKDERPAIGRRANEIKRALEASLAERDAALREGALQRSLQAERLDVTLPGRPL
                     TQGRLHPITQTYRQIARIFAEMGFQVYRSRDIETDEYNFQLLNFPPHHPAREMQDSFY
                     IDSPHEEDNPLVLRTHTSPGQIRSMREFSSSNPENPPPVRIILPGMCYRYEQITARSE
                     IQFSQVEGLVVGEHITFTDLKGTLSDFARRLFGGQVRTRFRASHFPFTEPSAEMDVEC
                     FVCGGLGCSVCKRSGWLEILGCGMVHPIVLHNGGYPP"
BASE COUNT          446 a          558 c          604 g          469 t
ORIGIN      
        1 gtggccaggt tgacgtattt ggtgcgcgtt ttgacctgct cgaagatctc ttcggcagtg
       61 gcgtgaccgc tggcatgggc gatggcctca acgatcatct cgcgctgagg ggtgatgcgg
      121 tagcccagat cacgcagggc ttggagtagg gtgtcacagt gggtcattgc gaaaactttc
      181 agttgcgaat atttgcaata agattatacc ggattaggta gagattgtaa aggacttgtc
      241 atgtggtcat aataaatcca agcccttgct acaggacagg ggtgttgtgg catgaaaaaa
      301 ggtatctgcc tatgcaaaca ccttttttct cgctaaaaat ggggatagat tatttggtca
      361 aatcgacgga ccgaacttgg cgataatgcg atccttgggc tggtaaccga aaatgcgctg
      421 gctgggctga ccattaacga acagcatcag cgtgggtaca ctcatcacct ggtagtgcat
      481 tgccaggttg gggttgctgt ccacatccag cttgaccact ttgactttgc cagcccactc
      541 ctgggcaagc tgcttgacca ccgggtcgag catcttgcac ggcgcgcacc ataccgccgt
      601 gaaatccacc agcacgggtt gactggcttc caacacctcc tgttgaaaat cggcttcatt
      661 cacgtgagcc aaatcgctca tcgaattacc tccatgcact attgggagac atggtaatca
      721 tactcgcccg aaaagtcaag gaagtcccca tcttgacaga accttctttt tgaagataat
      781 aggggcaaca accgacctgc aatagcttgg acggaggaaa gtaggctagc tgaagccgac
      841 agggaggcat ggggcgcaga ctgagacccc agcccggtgg aaactgtccg aagttccctc
      901 ctgagctgcc cgggtgaatg acgttccagg taccaagttc tactgttctg gacatagtgc
      961 ggcacttgga acatgaactg tttagtagtt tgggccgcga ccccggcgtt aaaaggggag
     1021 ctgatgaact cgtcagtccg agttggatcg ccttgggcga tcaagttggg tggtaccgcg
     1081 ggaatttgct cccgtccctt cgccgggatg ggagtttttc attatcttgg agggttgaat
     1141 gttacaaaaa ttggaagaga ttcaacgtgc cgcactcgat gcgcttgaag cggtggatga
     1201 tgagggtgcc ctggagaact ggcgtgttgc ctacctgggg cgcagcgccc cgttgatgag
     1261 cgtgtttgac caattaggca gcctgcctaa agacgagcgc ccggcaattg ggcggcgtgc
     1321 caacgagatc aaacgcgcct tggaggcctc tctggcggag agagacgcgg ccctgcggga
     1381 gggagcattg cagcgctcgc tacaggccga aaggctggat gtcaccctgc ctggtcgccc
     1441 gctcactcag ggtcgccttc accctatcac gcagacctat cgccagatag ctcgcatctt
     1501 cgctgagatg ggatttcagg tctaccgctc gcgagacatt gagacggatg aatataattt
     1561 ccagctgctc aacttcccgc cgcaccatcc tgctcgcgag atgcaagatt cattctatat
     1621 cgactccccg cacgaggagg acaacccgct cgtattgcgc acccatacct cccccggtca
     1681 gatccgctcg atgcgcgagt tctcgagctc taatcctgag aatccgccgc cggtaaggat
     1741 tatcctgccg ggtatgtgtt accgctacga gcagatcact gctcgctcgg agattcagtt
     1801 cagccaagtc gagggtctgg tggttgggga gcatatcacc ttcaccgacc tgaaggggac
     1861 gctgagcgac ttcgcccggc gtctatttgg gggacaggtg cgcacccgct tccgtgcttc
     1921 gcacttcccc ttcaccgaac ccagcgccga gatggacgtg gaatgcttcg tatgtggcgg
     1981 gctgggttgc tcggtctgca agcgcagcgg ctggctggag atcctgggat gcggtatggt
     2041 ccatcccatc gtgctgcaca acggcggcta ccctcct
//
LOCUS       DBNHOH010000421         4152 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_729419, whole genome shotgun sequence.
ACCESSION   DBNHOH010000421 DBNHOH010000000
VERSION     DBNHOH010000421.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_729419"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1279)
                     /locus_tag="AC16V7_07445"
     CDS             complement(<1..1279)
                     /locus_tag="AC16V7_07445"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6256991.1"
                     /translation="MYLNTLKKTLIFGLLILLLAFSLASCGQKEAEVPTEVTVIEPTA
                     VVEPTEQPAEEPTVAVEPTAEEAPMPEQSTIVILVPEDPTGFNGLLTDTGYEQLVGEL
                     VMLSVAEVDPMGTVIPEIAAEIPTLENGGVEFDEENWTMNVIWKIRDDVYWADGEQLT
                     VDDIIFSWNVITDPDNGTWTDGLDYTDSIEKIDDFTFKVFYNTVYTAYQTQFGGENFF
                     VYPEHYCDASQGFFEWDCDKEPLSSGPFMLEEWVTNDHLTFVKNPNYFEAGKPGIDRV
                     IVQIIPEQTVRKTLMLEGDADLHVWPPDAIWQEYENAPDLQAVKSPTERWVMRFIPNL
                     AARGETDPVENPHPFLSDVRVRQAIRHAINVDTLIEDIFLGHGVPVWTEFFRPPYECE
                     IPRPVYDLDGAAALLEDAGWTDTDGDGVRECHGC"
     gene            complement(1333..1983)
                     /locus_tag="AC16V7_07450"
     CDS             complement(1333..1983)
                     /locus_tag="AC16V7_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HIR6256992.1"
                     /translation="MEDEMYKKLAQAVIDGEPEDAVALAKEALAQGLDPMECITQGLT
                     KGIQEVGTLFSCGDYFLPELIIGAEAMKDALAILEPALLADQKRDVVANVVLGTVEGD
                     VHEIGKTLVGTMLTANGFKVTDIGVDKPAAEFIEAIKETGATIVGVSALLTTTMLQQE
                     KIIRALEEAGLRNEVKVMVGGAPVTASYAKQIGADGYADDAISAVALAMRLADAPA"
     gene            complement(2031..3482)
                     /locus_tag="AC16V7_07455"
     CDS             complement(2031..3482)
                     /locus_tag="AC16V7_07455"
                     /inference="COORDINATES: protein motif:HMM:NF018012.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR6256993.1"
                     /translation="MVEIQPIKSIYPFEALDADQLACIKDATLYVLDKVGVRFPSEKA
                     LRIFDEHGANVDFETKIVCMPPDLVLESMGKAPRNYTLSGRAEGTDLYLGNGYSYFST
                     DGCGHETLDFETGERRLSCKEDVAKMAWVCDYLSSIAFYWPIVSAQDFGQLAPLHEID
                     AAFNNTVKHVQTETVMGMGMAKYAIRMAEVVAGDMESLREAPVLSSLICTIAPLSQDR
                     EGIEGAMVFAEAGIPVGFMGMPNMGSTAPAVPGGALVVANAETVSAMVLMQLVAPGAP
                     VFQSILVSGMDPQSADYIVSSPEKYLCNVAAVQIAHDWGVPSLAGTFGVDCPEPASWQ
                     LGRDSVYTALLTALAGTDITIGLGMLKASTLLVPEQIIFDDKIYHIHRVLARGLDIRE
                     SSLAVDVIEAVGPAGHFLRQKHTRTNIRERWIPKLTHPSPSMDGTTTPHIRLRAKAEF
                     DRILKQHQPEPLSDRKQQELRTIIEIAQNDIGI"
     gene            complement(3505..>4152)
                     /locus_tag="AC16V7_07460"
     CDS             complement(3505..>4152)
                     /locus_tag="AC16V7_07460"
                     /inference="COORDINATES: protein motif:HMM:NF018012.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR6256994.1"
                     /translation="LYPGTPLLYGSVSAVLDMRTGILPLGAPERGMVNSGAAIMGHYY
                     GIPSMCGGLSSDAKELDAQAGFEKAVTAVPLLMEGASIIYGVGAVDAGSAISYSEMVM
                     DDEFIGALRRMMQGIAIHDLDEEVELIKRLTPRGNFLKEKHTRKNYHEHWQPHLLNRE
                     AYDTWAIDRKTVAQKARQRAENILQQHQPKPLPVKAEAEIERILSRYLVVAEGSP"
BASE COUNT          956 a         1146 c          962 g         1088 t
ORIGIN      
        1 gacaaccgtg acactctcgc acaccatctc catccgtgtc ggtccagcca gcatcctcga
       61 gtaatgccgc tgcgccatcg aggtcgtaaa ccggtctggg gatttcgcac tcatacggcg
      121 ggcggaaaaa ttccgtccac accggcacac catgacctag gaatatgtcc tcaatgaggg
      181 tatctacgtt aattgcatga cgaattgctt ggcggacgcg aacgtcgctg aggaatggat
      241 gtggattctc tactggatct gtctctcctc tggctgctaa gttcggaatg aaacgcatca
      301 cccatcgctc cgtgggggac ttcacggcct ggaggtcggg cgcgttttcg tattcctgcc
      361 aaatcgcgtc tggtggccaa acgtgcaaat cagcatcgcc ttccagcatc aatgtctttc
      421 gcacagtttg ctccgggatg atctggacga taaccctgtc aatccctggt tttccagctt
      481 caaagtagtt ggggttcttc acaaaggtca ggtgatcgtt tgtcacccac tcttccagca
      541 tgaatggacc gctgcttagc ggttctttgt cacaatccca ctcaaaaaat ccctgggatg
      601 catcgcaata atgctcagga tatacaaaga agttttcccc gccaaactgg gtctggtagg
      661 ctgtgtaaac cgtattgtaa aagaccttga aggtgaagtc gtctatcttc tcaatgctgt
      721 cggtgtaatc cagaccatct gtccaggttc cattatccgg atcggtgatg acgttccagg
      781 agaagatgat gtcatcaaca gtgagctgct caccatccgc ccagtagaca tcatcacgga
      841 tcttccatat cacattcatt gtccagtttt cttcatcgaa ctccacaccc ccattctcca
      901 gtgtcggaat ttccgcggct atttctggaa taacggtccc catgggatcg acttctgcta
      961 ccgacagcat gaccagctcg cctaccagct gctcataacc tgtatcggtc agcaaaccat
     1021 tgaagccggt tgggtcttct ggaaccaaga taacaatcgt ggattgttca ggcatcggtg
     1081 cttcttcagc agttggctcc acggcaacgg ttggttcttc ggctggttgc tcagttggtt
     1141 ccactacagc cgtcggctcg attactgtga cctcagtcgg cacttctgct tctttctgac
     1201 cacacgaggc cagagaaaat gccaggagga gaatcagcaa accgaagatt agggtttttt
     1261 tcagggtgtt cagatacacg tgtgcctcct tttgttattg ttgctcgaat ctaaccaaca
     1321 tttccagcaa atttatgctg gcgcgtcagc gagacgcata gccagagcca ctgctgagat
     1381 tgcatcatca gcatagccat cggctccgat ttgctttgca taactcgcgg taacaggtgc
     1441 tccaccgacc atgaccttga cttcattacg gagacctgct tcctccaagg cgcggattat
     1501 tttttcctgt tgtaacattg tggttgttag caatgccgat accccgacga ttgtggcgcc
     1561 cgtttccttg atggcctcga taaactctgc ggctggtttg tcaaccccaa tatctgtgac
     1621 cttgaagcca ttggcagtca acattgtccc caccagtgtc ttgccaatct cgtgtacgtc
     1681 accttcaaca gtaccgagca ccacattggc taccacatcg cgtttttgat ccgcaagcaa
     1741 agctggctcg aggattgcca aggcatcttt catcgcctcg gcaccgatga tcagctctgg
     1801 caaaaagtaa tcgccgcaag agaatagcgt accaacctct tgtatgcctt ttgtaaggcc
     1861 ttgcgtgatg cactccatgg ggtctaaccc ttgcgctagc gcctctttgg cgagcgctac
     1921 ggcgtcttcc ggttcgccat caattacagc ctgggctagt tttttataca tttcgtcttc
     1981 cataaattcc tcctttttct catacggtct ttgtcatctt cccccataag tcagattcca
     2041 atgtcatttt gcgcgatctc gatgatagtt ctaagttcct gctgttttcg atcgctaagg
     2101 ggctcgggtt ggtgttgctt tagtattcga tcgaattctg ccttagcccg caggcgtata
     2161 tgcggcgttg ttgtcccatc catcgatgga gaagggtggg tcaatttggg tatccaccgt
     2221 tctcgaatat tcgttcgcgt gtgtttttga cgcaggaaat gtccagctgg acccacagct
     2281 tcaatgacat caacagccaa gctcgactcc cgaatatcta atccccgggc aagcacacgg
     2341 tgaatgtggt aaatcttgtc atcaaagata atctgctcag gaaccagcaa ggtagatgct
     2401 ttcagcattc ctaaaccaat cgttatatcc gtcccagcca atgcagtcag caaagcggtg
     2461 tagacgctat cccgtcctaa ttgccagcta gctggttctg ggcagtccac accaaaggta
     2521 cccgccagcg atggcacacc ccaatcatgc gctatttgta cagctgccac attgcacaaa
     2581 tacttctccg gcgagctgac gatataatcc gccgattgtg gatccatacc ggaaaccagg
     2641 atggattgga aaaccggggc gccaggtgcc accagctgca taagaaccat cgcactcact
     2701 gtttcggcgt ttgccaccac caaggcccct cccggcacag ctggcgcggt tgatcccata
     2761 ttgggcatcc ccataaaccc aaccggtatc cctgcttcag caaagaccat tgccccttca
     2821 atgccttccc gatcctggct caatggagca attgtgcaaa taaggctgga aagcacaggg
     2881 gcctctctca gggattccat gtcaccggca accacctctg ccatcctgat tgcgtattta
     2941 gccattccca tgcccataac cgtttcggtc tggacatgtt tgaccgtatt attaaaagct
     3001 gcatcgatct catgcagagg tgccagctgg ccaaaatctt gagcgctgac gattggccaa
     3061 taaaaggcaa tcgaagaaag ataatcgcag acccatgcca ttttggctac atcttcttta
     3121 cacgatagac gtctttcgcc ggtctcgaaa tccagggtct cgtgtccgca gccatcggtc
     3181 gaaaagtaag agtagccatt gcccagataa aggtctgtcc cctcggcgcg tccagagagg
     3241 gtgtaatttc ggggagcttt gcccatcgac tccaaaacca aatccggggg catgcataca
     3301 atcttggtct caaaatccac attcgcacca tgctcgtcaa atatcctcag ggctttttcg
     3361 gatggaaaac gcacaccaac cttgtctagc acgtacaacg ttgcatcctt tatgcatgca
     3421 agctgatcag catcaagcgc ttcgaaggga tatatcgact ttatgggttg gatctcaacc
     3481 aatagttact cctatatagg gagttcacgg cgaaccctcc gctaccacta agtaacggct
     3541 gaggatacgt tcaatctctg cttcggcttt tacaggcaat ggttttggtt gatgttgttg
     3601 caggatgttt tcagctcgtt gacgagcttt ttgcgctacc gtcttgcgat caattgccca
     3661 ggtatcatag gcttcgcggt ttaagagatg cggttgccag tgttcatggt agtttttgcg
     3721 ggtgtgtttt tctttcagga aattacctct tggtgtgaga cgtttgataa gctcaacttc
     3781 ttcatccagg tcatgaatcg caataccctg catcatcctg cgtagggctc cgataaactc
     3841 gtcatccata accatctcgc tgtaagagat cgctgaaccg gcatctactg cgcctacacc
     3901 gtagatgatg ctcgcgcctt ccataagcaa aggcactgcc gtgaccgcct tttcaaaacc
     3961 tgcttgtgcg tctaattctt ttgcatccga ggataagcct ccgcacatag aagggatacc
     4021 ataataatgc cccatgatcg cggcaccact gttgaccata cctcgttctg gcgcgcctag
     4081 gggtaaaata cctgtccgca tatcaaggac agccgaaacg ctgccataca atagcggcgt
     4141 gccagggtaa ag
//
LOCUS       DBNHOH010000422         1677 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_408303, whole genome shotgun sequence.
ACCESSION   DBNHOH010000422 DBNHOH010000000
VERSION     DBNHOH010000422.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_408303"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(299..>1677)
                     /locus_tag="AC16V7_07465"
     CDS             complement(299..>1677)
                     /locus_tag="AC16V7_07465"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent decarboxylase
                     family protein"
                     /protein_id="HIR6256995.1"
                     /translation="QRYKSYDMDWKSGKVWAYVYNPGSEVMEVITKAHNLFLAENGLD
                     PSVFPSLLKLETDVVRMVANLLRGDEQVVGHVTTGGTESIMLAVKTARDKARFERPEI
                     TAPEMVLPKTAHAAFHKAAHFLGLKPVVTPFDPQTFRADVSAMRSAITENTVLLVASA
                     PCYSQGVIDPIREIGALAQEMGLPFHVDACVGGIHLSFMREMGYSLPEFDFSVPGVTS
                     ISVDMHKYGYAAKGCSTILYRDKSLRRYQIYACTDTTTYSLINPTVLSTKSGGPLAGA
                     WAILNYLGEAGYKRMVSEVQDATHRLIQGINHIEGLRVLGEPDMCMFSFTSDIINVYQ
                     LADEMKKRGWYLQGQFSTPLSPRNLHISVNHGTLPNVDAMLRDLGECAEIVKKAKPLD
                     TTFIRSQVASALENPSPETFANLAELAGIRGTELPNEMATINEVLDALPDEVANMLLI
                     EYFNELYV"
BASE COUNT          353 a          459 c          486 g          379 t
ORIGIN      
        1 taataacaat ctgacaagca tactgaatgg ggtggcgatg gcatttagcc tcttcttctt
       61 gtggctgctg attgcccagg tagtcatcct cacccaggga tgggaattgt accaaggcac
      121 ggcaagccgc atggagagca gtgaatcttg agcagtgcaa tataaaatct ttgccgaatt
      181 gtatattatc cgatatatcg atttgttttt ataggcaccc catggcattt tcccttgata
      241 ccacccttgg ccaactatga gacgaattaa gctcgggact gcgtaacaaa atatcccctc
      301 atacatatag ctcattgaaa tactcaatca acagcatgtt ggcgacctcg tccggcaggg
      361 cgtccagcac ctcattaatg gtagccatct catttggcag ttccgtgccg cggatgcctg
      421 ccagctctgc caagttggcg aaggtctccg gggaggggtt ctccagcgcg ctggcgacct
      481 gcgagcggat gaaggtggtg tcgagtggct tggctttttt gacgatctcg gcacactcac
      541 ccaggtcgcg cagcatggcg tcaacattgg gcagtgtgcc atgattgacc gagatatgca
      601 agttgcgcgg cgagagcggt gtggagaact gcccttgcag gtaccagccg cgctttttca
      661 tctcgtcagc cagctggtag acattgatta tgtccgaagt gaaggagaac atgcacatgt
      721 ctggctctcc cagcacgcgc agcccttcga tgtggttgat gccctggatg aggcggtggg
      781 tggcgtcctg cacctcgctc accatgcgct tgtaacccgc ctcccccagg tagttcagga
      841 tcgcccaggc gcccgccaac ggcccgcctg acttggtgct cagcacggtc gggttgatca
      901 gcgagtaagt ggtggtgtcg gtgcaggcgt agatctggta acggcgcagg ctcttatccc
      961 ggtacaggat ggtcgagcag cccttggcgg cgtacccgta tttgtgcatg tctaccgaga
     1021 tggaggtaac tccgggcacg ctgaaatcga actccggcag agagtagccc atctcccgca
     1081 tgaaagacag gtggatgcca cccacgcaag cgtccacgtg aaatggcagc cccatctcct
     1141 gggccagagc gccgatctcg cggatgggat cgatcacacc ctgcgagtaa cacggtgccg
     1201 aggccaccag caggacggta ttctcggtaa tggcagatcg catggcgctc acgtccgccc
     1261 ggaaagtttg tgggtcgaat ggggtcacca caggctttaa gcccaggaag tgagcagctt
     1321 tgtgaaaagc tgcatgggct gtcttgggga gcaccatctc gggagcagtg atttcagggc
     1381 gctcaaagcg ggctttatca cgggcagtct tcactgccag catgatgctc tccgtcccgc
     1441 cggtggtgac gtgacccacc acctgctcat cgccgcgcag caggttggca accatgcgca
     1501 ctacatctgt ctccagcttc aacaggctcg ggaaaacgga tggatcgagg ccgttctcgg
     1561 cgaggaacag gttgtgggct ttggtgatta cttccataac ctcggagcct gggttataca
     1621 cgtatgccca tactttgcca gatttccagt ccatgtcgta ggatttatag cgttgca
//
LOCUS       DBNHOH010000423         2397 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_922021, whole genome shotgun sequence.
ACCESSION   DBNHOH010000423 DBNHOH010000000
VERSION     DBNHOH010000423.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_922021"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1632
                     /locus_tag="AC16V7_07470"
     CDS             127..1632
                     /locus_tag="AC16V7_07470"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008682076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR6256996.1"
                     /translation="MNQDDTRAATETGEGSGTGQELDESVVEPGNALPEVTMADLPES
                     WLQAMGRAGWSKLMPVQAKAIPYIMAGRNLLVQSRTGTGKTGAFILPMLERINPLLDA
                     CQVLILVPTRELAQQVARDAEVLAGDRGIRIIAVYGGVRYGAQLKAFQEGAHLVIGTP
                     GRVLDHLFKQSLSLEKLQILLFDEADRLLSMGFYPDMREVQRFLPPHPVEGYMFSATF
                     PPRVMRLAEQFLGKSEFLGLSSGAVHVAGTEHVYYSIPGMEKDRGLVRIIEIENPDSA
                     IIFCNTRAKVHYVTVVLRRFGYDADELSADLSQNERDKVLTRVRRGKLRFLVSTDVAG
                     RGIDIPELSHVIQYEPPEDPEVYIHRAGRTGRAGAAGEAITLVDVTEQINLRRIAKQY
                     QIDFEERPLPTREDVAAVVSERMTVFLETQLRTRDRLKTERMQRFVPLAQSLSSSEDE
                     LPLITMLLDDYYQQSLHTPPPQPPGAEEPPKKRSDDGHSKRKGRRGGRRRN"
     gene            1651..1791
                     /locus_tag="AC16V7_07475"
     CDS             1651..1791
                     /locus_tag="AC16V7_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6256997.1"
                     /translation="MHFAGSDQQGEMPRGGALRHTQVEGAEVFDWDFYQRVSGDATCP
                     PA"
     gene            complement(1772..>2397)
                     /locus_tag="AC16V7_07480"
     CDS             complement(1772..>2397)
                     /locus_tag="AC16V7_07480"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6256998.1"
                     /translation="RTAVENEVVLYAVNWPSPIFDMAAREDGAGFRRVSELVGCALSY
                     AQSTAADYRGDPDNVALVGFSMGAMYGAWLALGQDALEPAWERFEQARGGPPTQVSCE
                     LAAGVPHLQTFIAIGGTMYGFDPLRSRDAEIWELVSAYSYIGRNPDLKVRFLAGERDA
                     EGVTLEANRLNDALAEAGYDTAVISYDGAHVLPPELAISEILKLAGR"
BASE COUNT          519 a          699 c          695 g          484 t
ORIGIN      
        1 acgcgcaccc gggcccgtca atacagacct ggcgcttttc cagttctttg aagacctcgg
       61 ccaccctgtt tgctgcccat caagcaacgc tgtatcatcg cttcatagca acaattcgga
      121 gaacacgtga atcaagacga tactcgcgct gctactgaaa ccggcgaagg tagcggcaca
      181 ggacaagaat tggacgaaag cgtggtcgag cctgggaacg cgcttcccga ggtgaccatg
      241 gctgatctgc cggaatcgtg gcttcaggcg atggggcggg ccggctggtc caagctgatg
      301 ccggtgcagg ccaaagcgat tccctacatc atggccggtc gaaacctgct ggtgcagtca
      361 cgtaccggca ccggcaagac gggtgctttt atccttccca tgctggaacg gataaatcct
      421 ctactggatg cctgccaagt tctgatactg gtacctaccc gggaactggc acagcaagtc
      481 gccagggacg ctgaagtcct ggctggggac aggggcatcc gcatcatcgc cgtctatggt
      541 ggcgttcgct atggggcgca gctcaaggca tttcaagagg gagctcacct ggtcatcggt
      601 acgcctggtc gcgtgctcga tcatctattc aagcagtcgc tgtctcttga aaagctacag
      661 atcttgcttt ttgatgaagc ggatcggcta ttgtctatgg gtttctaccc cgacatgcga
      721 gaagtgcagc ggttcctgcc tccccacccg gtcgagggtt atatgttctc ggccactttc
      781 ccgcctcggg tgatgcgcct ggctgaacag ttcttgggca agtcggagtt tctaggattg
      841 agcagcggcg ccgtacatgt ggcagggacg gagcacgtct actactcaat tccagggatg
      901 gaaaaagatc gcggtttggt gcgaatcatt gagatcgaga atcctgactc ggccatcatc
      961 ttctgcaata ccagggccaa ggtgcattat gtgaccgtcg tcctacgtcg attcggctac
     1021 gatgccgacg agctaagcgc tgatctttcg caaaatgagc gggacaaggt attgacacgg
     1081 gtacgccggg gaaaactacg cttcctcgta tccaccgatg tggccggccg tggcattgac
     1141 atacccgaac tatcccacgt gatccaatac gaaccaccgg aagatcctga agtttatatc
     1201 catcgagccg gccgcacagg gcgcgctggc gctgccggtg aagcgatcac cttggtagat
     1261 gttaccgaac agatcaatct caggcgcatc gccaagcagt accagatcga ttttgaggaa
     1321 cggccgttgc ctacccgaga agatgtcgcc gctgttgtgt ccgaacgaat gaccgttttc
     1381 ttggagaccc aactccgcac ccgcgacagg ctgaagacag agcgtatgca gcgcttcgtt
     1441 cccctggccc aaagcttgag cagcagtgaa gatgaattgc cgttgatcac gatgctgctg
     1501 gatgattatt accagcaatc gcttcacacc cctcccccac agcctccagg agcggaagaa
     1561 cctcctaaga agcgctccga tgacggccac agcaaacgaa aggggcgtcg cggtgggcga
     1621 cgtcgcaatt aaaggcgtgt tgattggctg gtgcacttcg ctgggagcga ccaacaggga
     1681 gagatgccgc gcggcggcgc gctacgccac acccaggtcg agggggcaga agtcttcgac
     1741 tgggacttct accagagagt aagtggcgat gctacctgcc cgccagcttg aggatctctg
     1801 aaatggcgag ttcaggcggg aggacgtggg cgccgtcgta cgagatcacg gcggtgtcat
     1861 atcccgcttc tgccagagca tcgttcaggc gattggcctc cagggtgaca ccctcagcat
     1921 cccgttcccc ggccaagaag cgaaccttta ggtccgggtt ccgccctatg taactgtaag
     1981 cgctgacaag ctcccagatc tcggcgtctc tactgcgcaa cgggtcaaag ccatacatgg
     2041 tgccaccgat ggcgataaag gtttgaagat gaggcacgcc tgcggccagc tcacaagata
     2101 cctgggtcgg cgggccaccc cgtgcctgtt cgaatcgttc ccaagcaggt tccagcgcgt
     2161 cttggcccag ggccaaccat gccccataca tggcccccat agagaagcca acgagggcga
     2221 cattgtcggg atcgcccctg tagtcggcgg ccgtggattg ggcatagctg agggcacagc
     2281 ccaccagttc ggacacccgg cgaaatcctg cgccatcttc cctggctgcc atatcgaaaa
     2341 tcgggctggg ccagttcacc gcgtatagaa ccacttcatt ttcaaccgca gtccggt
//
LOCUS       DBNHOH010000424         3571 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1562381, whole genome shotgun sequence.
ACCESSION   DBNHOH010000424 DBNHOH010000000
VERSION     DBNHOH010000424.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1562381"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="AC16V7_07485"
     CDS             <1..1017
                     /locus_tag="AC16V7_07485"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HIR6256999.1"
                     /translation="TLEADYVLVSIQDGESGPQKTEVSIGVDIPEKARHLINGILQSV
                     LTSKEPVLLESASGGMASAAGLHAMLAAPLVVQESRKDGPALGVILAASRRTRAFGQR
                     QLSILQMIAGQVSLVVQNVRLLAQLEYKTMIEERMRLAREIHDGLAQTLGFLKLKTAQ
                     MRSYLEQGEGDLLQEAVDTCYSVLAEAYQDARQSIDGLRISSKEGFGEWLQQTVDEFQ
                     EHTGMLVDLCDSQIAGALLPEVQVQLIRIVQEALNNVRKHAKARQVEIDCQRVGQDMV
                     MEIRDDGGGFNVEDVPGPSQHGLRGMRERAELIGADFQVISQPEKGTTVRIRLPVQER
                     ERLL"
     gene            1014..1655
                     /locus_tag="AC16V7_07490"
     CDS             1014..1655
                     /locus_tag="AC16V7_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6257000.1"
                     /translation="MKPISLLVVDDHALFRSGLISLLKGMPEFEVVGEAGNGQEALEA
                     ARQLAPDMVLLDVNMPVMDGVEAVRAIKAALTCRIMMLTISKNDDDLFGSILAGADGY
                     LLKNATPDELRKAILQVHGGMAVLAPDVTRKVMGAVRTDPASLASHGLSSREMEVLQC
                     LAQGLTTNQISNELFISDNTVKTHVRHIMEKLEASNRAEAVSKATQMGLIGGE"
     gene            1779..3221
                     /gene="coxB"
                     /locus_tag="AC16V7_07495"
     CDS             1779..3221
                     /gene="coxB"
                     /locus_tag="AC16V7_07495"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HIR6257001.1"
                     /translation="MTQRARPQRILIASSHALFGQGLRSLLLARQKAEVEVVGMVSSL
                     GEALAAIDVLNPDLVIVDYDDQALNREEFLARFVGSEKKLRVVLLSLQNPEEAIVYDR
                     RTMAAAQIDRWLEEWPTEEEAEAPPPPSGGGPLSANRLRRNTMKHLIIAGIFVVLVTV
                     LLIVGMDTAGLLPEQASLQAIPIDSMFDLEFKVIYFLFALIVVFMVYSIVVFRRKPGD
                     TSDARHVEGNTKLEVTWTLIPLVTVLTFSFLGAQSLAETQAIGDSVMEVNVIGSQWAW
                     RFEYPDTGIVSNELRLPVGKQALLHLQSTDVIHSFWVPEFRVKQDALPGGEEFIRDLR
                     VTPTKIGEYKVRCAELCGLNHAYMEAPVIVMAEADYKDWEIAESGLSPDPVVRGQKWY
                     ENYGCNACHTLDGTIKVGPSWKGLYGSQAKFTDGTTAVADDAYIYESIRNPGAKTVEG
                     FTPGLMPQNIAEKMSDDQISDVIEFIKTLK"
     gene            3269..>3571
                     /locus_tag="AC16V7_07500"
     CDS             3269..>3571
                     /locus_tag="AC16V7_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257002.1"
                     /translation="MSENNRVNLFSTSLVRGLLAMVIGIPVGMGLVTLVRLLVGLPAW
                     KAEPAWVVGAVFGVVAFMLGTGVMNDWLKWARGEETPEHPIANPNLKGLAKYLSVSF"
BASE COUNT          760 a          924 c         1132 g          755 t
ORIGIN      
        1 acgctggaag ctgattatgt gctggtttcg atacaggatg gtgagagcgg accccaaaaa
       61 acggaggtct cgatcggggt ggatatcccg gagaaagccc gccacctgat caacgggatt
      121 ttgcaaagcg tgctgacgtc caaagagccg gtcttgctgg agagcgcctc tggggggatg
      181 gcatccgcgg cagggttgca cgccatgctg gctgcgccgc tggtggtcca agaatccagg
      241 aaagacgggc cagcgctggg ggtcatcctg gctgccagcc ggcgaaccag ggcgttcggt
      301 cagcgccagc tgtccatcct gcagatgatc gccgggcagg tctccctggt ggtgcagaac
      361 gtgcgcctgc tggcccagct tgagtataag acgatgatcg aagagcgcat gcgcctggcg
      421 cgggagatcc atgatggtct ggcgcaaacc ctgggattct tgaagctgaa gaccgcacag
      481 atgcgctcgt atctcgaaca gggtgaaggc gatttgctcc aagaagcggt agatacctgt
      541 tatagcgtgc tggcagaagc ctaccaggac gctcgccagt cgattgacgg gctgcgcatc
      601 tcttccaagg aaggctttgg agagtggttg cagcagacgg tcgacgagtt ccaggagcac
      661 actggcatgc ttgtcgatct gtgcgactcg cagatcgctg gcgctcttct gcctgaggtc
      721 caggtgcagt tgatccgcat cgtccaggag gccctgaaca acgtgcgcaa gcatgccaaa
      781 gcccggcagg tggagatcga ctgccagcgt gtgggccaag acatggtgat ggagatccgc
      841 gacgacggcg gggggtttaa tgtggaggat gtgcctggtc catcgcagca cgggttgcgc
      901 gggatgcgcg agcgggctga gctgatcggc gctgatttcc aggtgatcag ccaacctgag
      961 aaggggacaa ccgtccgcat ccggctgccg gtgcaggaga gggagaggct gttatgaaac
     1021 cgatcagctt gctggtggtg gatgatcatg ccctgttccg ctcggggttg atcagcctct
     1081 tgaaggggat gccagagttc gaggtggtgg gcgaggcagg caacggccag gaagccctgg
     1141 aggccgcccg ccagcttgcc cctgatatgg tcttgctgga tgtcaacatg ccagtcatgg
     1201 atggggtgga ggcggtgcgc gccatcaaag cggcccttac ttgccgcatc atgatgctga
     1261 ccatctccaa aaatgacgat gacctgtttg gctcgatcct ggctggcgcg gatggctacc
     1321 tgctcaaaaa cgccaccccg gacgagcttc gcaaagccat cctccaggtg catggtggca
     1381 tggcggtgct ggcgccagat gtaacccgca aggtgatggg cgcggtgcgc accgacccgg
     1441 cgagccttgc cagccacggg ttgagcagcc gggagatgga agtgttgcag tgcctggcac
     1501 agggcttgac caccaaccag atatccaatg agctgtttat ctcggataac accgtcaaaa
     1561 cccacgtgcg gcacatcatg gaaaagctgg aagcttccaa ccgggcggag gcggtcagca
     1621 aagccaccca gatgggtttg atcgggggag agtagaagcg caagttgtat cttcggaggg
     1681 gtgatgaaaa aatcacccct ccgggttagt taaatccctc ttttatgaca agggtggtgg
     1741 atgacatttt agaccttctt tggtaaatta taggaggtat gacacagcgc gccagacccc
     1801 agcgcatttt gatcgcctct tcccacgctc tctttgggca ggggttgcgt agtctgttgc
     1861 ttgcccgcca gaaagcagag gtcgaagtag tgggtatggt ttccagcctc ggcgaggcgc
     1921 tggcagctat cgacgtgctc aaccccgacc tggtcatcgt ggattacgac gaccaggccc
     1981 tcaaccggga ggagtttttg gcgcgttttg tgggcagtga gaagaaatta cgcgtggtct
     2041 tgctctcgct acaaaaccca gaagaggcca tcgtctatga ccgccgcacc atggcggccg
     2101 cccaaattga ccgctggctt gaggagtggc ccaccgaaga agaagcggaa gcgccgccgc
     2161 ccccctctgg gggtgggcct ctttccgcca accgtctcag gagaaacact atgaaacatt
     2221 tgatcattgc tggtattttc gtcgtccttg tcacggtttt gctgatcgta gggatggaca
     2281 cagccgggct cttacccgaa caggcaagcc tgcaagccat ccccatcgac tcgatgtttg
     2341 acctggaatt caaggtgatc tacttcctgt tcgccttgat tgtggtgttc atggtctaca
     2401 gcatcgtggt attccggcgt aagcccgggg atacatctga tgcccgccat gtggagggca
     2461 atacgaagct cgaagtgacc tggaccctca tcccgctggt gaccgtgctg actttttcct
     2521 tcttgggagc ccagtctctg gctgagaccc aggcgattgg ggatagcgtc atggaggtga
     2581 acgtgattgg ttcgcagtgg gcctggcgct ttgaataccc cgatacggga atcgtatcca
     2641 atgaattgcg cttgccggta ggcaagcagg cacttctgca cctgcaatca acggatgtga
     2701 tccattcctt ctgggtgcct gagttccgcg tcaagcagga cgccctgccg ggtggagagg
     2761 agttcatccg cgatctgcgc gttactccga ccaaaattgg cgagtacaag gtgcgctgcg
     2821 ccgaattgtg cggattgaat cacgcttaca tggaagcgcc agtgatcgtt atggcagaag
     2881 ctgactacaa agattgggag atcgccgaat caggtctctc gccagaccct gtggtgcgcg
     2941 gccaaaagtg gtacgaaaac tacggctgca atgcctgcca tacgttggac ggaaccatca
     3001 aggttggtcc gagttggaag ggtctctatg gaagccaggc taagttcacc gatgggacaa
     3061 cggctgtcgc ggatgacgcc tatatttacg aatccattcg caacccaggc gccaagacgg
     3121 tggagggttt cacacctgga cttatgccgc agaatatcgc cgagaagatg agcgacgatc
     3181 aaatcagcga tgtgatcgag ttcatcaaga ccttgaaatg ataatgagat acccgcaatg
     3241 acggcacaat catataagga gtttgggtat gagtgaaaac aacagagtta atcttttctc
     3301 caccagcctg gtgcgtggct tgctggcgat ggttattggt attccagtag gcatgggctt
     3361 ggttaccctg gtgcgcctgc tggttggctt acccgcttgg aaggccgaac cggcttgggt
     3421 ggtgggtgcg gtgtttggcg tggtagcttt catgctgggc acgggcgtga tgaatgactg
     3481 gcttaagtgg gcgagagggg aagagacacc cgaacaccct atagccaatc caaacctgaa
     3541 gggtctggcc aagtacttga gtgtctcgtt t
//
LOCUS       DBNHOH010000425         2501 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_152009, whole genome shotgun sequence.
ACCESSION   DBNHOH010000425 DBNHOH010000000
VERSION     DBNHOH010000425.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_152009"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(401..1945)
                     /locus_tag="AC16V7_07505"
     CDS             complement(401..1945)
                     /locus_tag="AC16V7_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HIR6257003.1"
                     /translation="MLARVYSCAVIGLEGVIVEVEVDTGQGLPGMTIVGLPDAAVQES
                     RERVQAAVRNAGFEYPRKRIVVNLAPATVRKEGPAYDLPIALGVLVMSERIPQEAVEE
                     TLVIGELSLDGNVRHARGVLPMAAIARQEGFKRIFVPEADAAEAALIPDLEVIPVPSL
                     AALYLHLCGQARLAAQPSITPENLPLLPQTDFREVKGQEHVKRALEVAAAGGHHALLV
                     GPPGAGKTLLARAMPGILPHMSIDEALDVTRIYSVADLLPSDVPLIRSRPFRAPHHTI
                     SHAGLVGGGNWPHPGEISLAHRGVLFLDELPEFGPRVLEVMRQPIEDKMVTISRAQGS
                     LTFPANFQLVAAMNPCPCGYYGDPVKPCTCAMGMVTKYQKRISGPLLDRIDIHIEVPR
                     VEYEKLSDQRMGEPSDVIQQRVEAARQIQRRRFAGEDEPGGADLHAATCNADMHLAEV
                     RKYCILDETGSSLMKTAMQQLQLSARGYHRILKLARTIADLAGAEQIAPAHLAEALQY
                     RPKMVI"
     gene            1980..2252
                     /locus_tag="AC16V7_07510"
     CDS             1980..2252
                     /locus_tag="AC16V7_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257004.1"
                     /translation="MPRFAPWPTLAKTMTNQFRSDIVPGEQRISLQVDVGGTLIGKAS
                     SIFNACMGLPKEGFDAYMAATCAAAVGRGWVGYDTGTGKHLALASQ"
BASE COUNT          553 a          705 c          727 g          516 t
ORIGIN      
        1 taatccttcc aaaagcacct ccattctggg tgtgcaactc tgttcaaaaa aaaacacacg
       61 gggcagcatg ggcctgtcgt gtgtttgtta cttccggcta ttttcggcga aggggagcgt
      121 tgtatgcggc gagtgtctgc acatcctgca taattctcaa aataagacaa ataagagtgg
      181 tttgatatta tagcatgaat tagctagaat aaattaataa gcgataataa aaatctaatt
      241 tatccggaat aatatagtga aattgcgggg gaagagttcg agcatgtgtt cgctcgcgcg
      301 tatcatcaat tcgtctagat taatcgtata gggagccagg gtgcagccgg ctgaataaaa
      361 tcaataaaag ccttcaccct agtggaggct cgatgttgga tcagatcacc atcttcggcc
      421 ggtactgcaa agcctctgcc aggtgcgccg gggcgatttg ttccgcgccc gccaggtcgg
      481 cgatcgtgcg cgccagtttc aatatacggt ggtaaccccg cgccgagagc tgcagctgct
      541 gcatggcggt cttcatcagc gaactacccg tctcatccag gatgcaatac ttgcgcactt
      601 ccgctaggtg catgtcggcg ttgcaggttg ccgcatgcag gtcagccccc cccggctcat
      661 cttcgcccgc aaagcgcctg cgctggatct ggcgcgccgc ttcgacccgc tgctggatga
      721 catcagacgg ctcgcccatt cgctggtcgc tgagcttttc gtattccacg cgtggcactt
      781 cgatatggat gtcgatgcga tcgagcagcg gaccagagat gcgcttctga tatttcgtca
      841 ccatacccat agcgcaggta cagggtttga ccgggtcacc ataatagccg catggacagg
      901 gattcatagc agcgaccaac tgaaaattgg ccgggaaggt cagcgatccc tgtgcccggc
      961 tgatggtgac cattttatcc tcgatgggct ggcgcatgac ctccagaacg cgcgggccga
     1021 actcgggcag ctcatccagg aagagtacgc cgcggtgcgc cagcgagatc tcccccgggt
     1081 gaggccagtt gccgcctccc accagcccgg cgtgagagat cgtatggtgc ggggcgcgga
     1141 agggacggct gcgaatcagg ggcacatccg acggcaacag gtcggcgacc gagtagatgc
     1201 gggtcacatc cagggcctcg tcgatgctca tgtgcggcag gatgcccggc atggcgcgcg
     1261 ccagaagggt tttgccagct ccaggcggcc cgacgagaag cgcgtggtgc ccacccgccg
     1321 cagccacctc cagagcgcgc ttgacatgct cctgaccttt aacctcgcga aaatcggtct
     1381 ggggcaaaag gggtagattc tcgggtgtga tggatggctg cgcagccaag cgggcttgcc
     1441 cgcatagatg caggtaaagc gccgccagcg acggcacagg gataacttcc agatcgggga
     1501 tcagggctgc ttcagcggcg tcggcttcag gcacaaaaat acgcttgaaa ccctcctgcc
     1561 gggcgatagc cgccatgggt agcacaccgc gggcgtggcg cacattgcca tccagcgaga
     1621 gctcgccgat caccagggtc tcttccaccg cttcttgggg aatgcgctcg ctcatgacca
     1681 gcacacccag ggcgatgggc aggtcatatg ccgggccttc cttgcgcact gtcgccgggg
     1741 ccaggttgac cacgatacgc ttgcgcgggt actcgaagcc agcgttgcgc accgccgcct
     1801 ggacacgctc tcgactctct tgcacggcgg catccggcag cccgacgata gtcatcccgg
     1861 gcaacccttg cccggtgtcc acctcgactt cgacgatcac cccctctaac ccaatcacag
     1921 cacaagaata tacgcgggcc agcataaggg tagtttagac tgcgtgggac gaaaagtcaa
     1981 tgccgcgttt cgcgccttgg cctaccctcg ccaagaccat gacaaaccaa ttccggagcg
     2041 atattgttcc aggagaacag cgtatcagct tacaggtaga tgtgggcgga acactaatag
     2101 gaaaggcttc aagtattttc aacgcttgta tgggcttgcc caaggaggga tttgatgcgt
     2161 atatggccgc tacctgtgca gccgccgtgg gacgaggatg ggtgggatac gataccggaa
     2221 ccggaaaaca cctagctctt gcctcgcaat aaaccatgtc ttaagtacct tgcatatctt
     2281 tttgccagct ctcttgaatt acgatggatg ggttcgaagt tacctcatcg gttactaagc
     2341 taacatcggg cggtcgttga gaaccacacg accgagggtg ctatgcaatg gcgcgaaaac
     2401 aaatagggtg atctgggcgc agagtcaatg aatcgttgtt gtataggcac agggcttgcc
     2461 tgcctagtta tcaggcaagc cctgtgccga tggagaactc c
//
LOCUS       DBNHOH010000426         3174 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_280276, whole genome shotgun sequence.
ACCESSION   DBNHOH010000426 DBNHOH010000000
VERSION     DBNHOH010000426.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_280276"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1757)
                     /locus_tag="AC16V7_07515"
     CDS             complement(<1..1757)
                     /locus_tag="AC16V7_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR6257005.1"
                     /translation="MNVLEVFKTMDYGPAPESPAAINAWLEDHERKFGLFINNQWVFP
                     DGSQTFASNNPATGEKLADTVQAGQEDVDAAIAAARQAFNTWSQTPGSVRARYLYAIA
                     RNIQKHHRLLAVLESMDNGKPLRESRDIDVPLLARHFYYHAGWAQLMETELSDYKPVG
                     VIGQIIPWNFPLLMLAWKIAPAIAMGNTVVLKPASYTRLTALLFAEVVAEAGLPPGVV
                     NVITGSSRAGTMIVTHPDVDKIAFTGSTDVGRILRRQTAGSGKKLSLELGGKSPFIVF
                     EDADLDSAVEGVVDAIWFNQGQVCCAGSRLLVQENVADRFIHKLKARMENIRVGDPLD
                     KCIDMGAIVDKSQWDTIDGWVKTGIQEGGVCFQPHIKLPDKGLFYPPTLITNLDAAAS
                     TVQEEIFGPVLVSLTFRTPSEAIALANNTRYGLAASVWSDNINLALDVASKVKAGSLW
                     VNCTNLFDAASGFGGYRESGFGREGGREGLFEYLRPAWQDRPRPEIQPQTEEKSGWGA
                     YIPARPSMPSDGRVNGKEMPHVDRTPKMYIGGKQVRPDGHYTRAVLGRKGEIVGQVGD
                     GNRKDIRDAVEAAHAAHTA"
     gene            complement(1788..2750)
                     /gene="deoC"
                     /locus_tag="AC16V7_07520"
     CDS             complement(1788..2750)
                     /gene="deoC"
                     /locus_tag="AC16V7_07520"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009565111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIR6257006.1"
                     /translation="MASKDDRNPGMPLDLDWVAGAHVNRSAVERRVATLPARRSIKKD
                     WQAAWLLRAITCIDLTTLAGDDTPGRVNRLCAKARQPLRPDMLAALAAQDQNITVGAV
                     CVYPSRIRDAVEALKGTNIPVASVATGFPAGQTPLPQRIAEIEQAIEAGAREIDVVIT
                     RTHVLTGNWGALYDELRQFRATCGNAAHMKTILATGDLGTLKQIYQASLVAMMAGSDF
                     IKTSTGKERVNATLEFGLVMTRAIREYWERTGYKVGFKPAGGISKAKQTLAWQALMRE
                     ELGVEWLMPDLFRIGASDLLTDIERQLYHYISGHYAARHHMPMG"
BASE COUNT          686 a          911 c          875 g          702 t
ORIGIN      
        1 ctggcggtat gcgctgcatg agccgcctcc accgcatcgc ggatatcctt gcggttgccg
       61 tcacccacct gcccgacgat ttcgcctttc cggcctaaca cggcacgcgt gtagtgccca
      121 tctggacgca cctgcttgcc gccaatatac atcttaggcg tgcgatcaac atggggcatc
      181 tcttttccat tcaccctgcc atcggagggc atactgggac gggcaggtat atatgccccc
      241 cagcccgatt tctcctcggt ctggggctgg atttcgggac ggggacgatc ctgccaggca
      301 gggcgcagat actcaaataa gccttccctg cccccctcgc gcccaaagcc actctctcgg
      361 taaccgccaa agccggaagc cgcatcgaag agattggtgc agttgaccca caatgaccct
      421 gccttcacct tgctggcaac atccaatgcc aggttgatgt tatcgctcca aacgctggca
      481 gccaatccat agcgcgtgtt gttcgccagg gcgatggctt cggagggcgt gcggaaggtc
      541 aaagagacca gcaccggccc gaatatctct tcctgcacag tagacgccgc cgcatccaga
      601 ttagtgatta gcgtcggcgg gtagaagaga cccttatcgg gcaatttgat atgaggctgg
      661 aagcaaaccc cgccttcctg gatgcctgtc ttgacccagc catcgatggt atcccattga
      721 gacttgtcga caatggctcc catgtcgatg catttatcca ggggatcgcc aacgcggata
      781 ttttccatgc gcgctttgag tttatggatg aaacgatcgg caacgttctc ttgcaccagc
      841 agtcgcgacc ccgcgcaaca cacctgaccc tggttaaacc agatggcatc caccacgccc
      901 tctacagcgc tatcgagatc agcatcctcg aagacgatga agggggactt gccacccagc
      961 tcaagggata acttcttgcc ggagcccgct gtctggcggc gcaggatgcg acccacatcg
     1021 gttgaaccag taaaggcgat cttatccaca tcggggtgag taacgatcat cgtgcctgcc
     1081 cggctgctgc cggtaatcac gttgaccacc cccgggggaa gcccggcttc ggcaaccacc
     1141 tcggcaaaga gcagggcagt gaggcgggtg taggaggccg gtttgagcac gacggtgttg
     1201 cccatggcga tagccggggc gatcttccag gcaagcatga gcaaggggaa attccacggg
     1261 atgatctgcc caatcacgcc aaccggcttg tagtcgctca gctcggtctc catcaactgt
     1321 gcccaacccg catgatagta aaagtgacgc gccagaaggg gcacatcgat gtcacgcgat
     1381 tcgcgcaggg gtttgccgtt gtccattgat tcgagcaccg ccaggagacg atggtgcttt
     1441 tgaatgttgc gggcgatagc atacaggtag cgcgctctca ctgaaccagg agtctggctc
     1501 catgtattga acgcctgtcg agccgcagca atcgccgcat ccacatcttc ctgcccggct
     1561 tgcaccgtgt cagccagctt ttcaccggtg gcggggttat tgctggcgaa ggtttggctg
     1621 ccatcaggga agacccattg attgttgatg aagagcccaa acttacgctc gtgatcttct
     1681 agccaggcat ttatagcggc tggcgattcg ggagcggggc cgtaatccat tgtcttgaag
     1741 acttctaata cgttcatgtt tggctccatt tcagttagcc cttataacta tcccatcggc
     1801 atgtggtggc gtgcagcgta gtgccccgag atgtagtgat atagctgtct ttcaatgtca
     1861 gtaagcaaat cgcttgcgcc aatgcggaac aggtcgggca tcaaccattc gacgccaagt
     1921 tcctctctca tcagcgcttg ccaggccagg gtttgcttgg ctttgctgat gccgcctgcc
     1981 ggcttgaagc cgaccttgta gccggtgcgt tcccaatatt cgcggatggc gcgtgtcatt
     2041 accaggccga attcgagggt ggcgttgacg cgctcctttc cagtggaggt cttgatgaaa
     2101 tccgagcccg ccatcatggc caccaggcta gcctggtaaa tctgtttcaa agttcccaga
     2161 tcaccggtag ccaggatcgt tttcatgtgg gcagcgttac cacaggtcgc acgaaactgc
     2221 ctgagctcat cgtaaagagc cccccagttc cctgtaagga catgtgtgcg cgtaatcacc
     2281 acatcaatct ccctggcgcc ggcctcgatg gcttgctcga tctcggcgat gcgctgaggg
     2341 agcggcgtct gaccggcagg gaagccggtc gccaccgaag cgacggggat atttgtgcct
     2401 ttgagcgctt caacagcatc cctgatacga ctgggataca cacacacagc ccccaccgtg
     2461 atgttctgat cttgggcagc caaggccgca agcatgtcag ggcgtaacgg ttgcctggct
     2521 ttggcgcaca ggcgattcac cctgccgggg gtgtcatctc cggcgagcgt ggtgagatcg
     2581 atgcaagtga tggcgcgcaa cagccaggct gcttgccagt ctttcttgat cgaccgccga
     2641 gccgggaggg tagcaacacg ccgctccact gcgctgcgat tgacgtgcgc gcccgccacc
     2701 caatccagat cgagcggcat accgggattg cgatcatctt tggaagccat cgttcaatcc
     2761 tttttgatcc tttgcgtaaa tattgccttt agaaattgcg caatcagagc ttctttgggt
     2821 caactgcgca agttattgtt ttttaattgt accaaaataa cttgcagcat gcggttatgc
     2881 aaagcagggc aattgcataa tccctgccag gcatgtgaaa ccctgcaaaa cgtggggtgg
     2941 ggggtaatca ctccaatgcg attggcttaa tacatagatc aacccaagtg atcccacagg
     3001 aaagatgcta aaataaatga gaccatgtcg ttgatatagc tctactgaat aacgctgaag
     3061 aataaacgtg attgagctta aggaacttca aaaagtaatc ggtgagaaca ccgtactgga
     3121 tattcccagt cttgccatac aagatggcga gatcgctgcc ctggtcggcc cggc
//
LOCUS       DBNHOH010000427         4724 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_473376, whole genome shotgun sequence.
ACCESSION   DBNHOH010000427 DBNHOH010000000
VERSION     DBNHOH010000427.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4724)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_473376"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..348)
                     /locus_tag="AC16V7_07525"
     CDS             complement(79..348)
                     /locus_tag="AC16V7_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257007.1"
                     /translation="MTPERQVYSVHGHYAGYLSDDPRILRKQSSGYLKPRQSPPRSPM
                     PIHPPATIPLAPMMPELPIGVNDVLEELPDMLPAVDFGDLREDMD"
     gene            complement(485..946)
                     /locus_tag="AC16V7_07530"
     CDS             complement(485..946)
                     /locus_tag="AC16V7_07530"
                     /inference="COORDINATES: protein motif:HMM:NF016060.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HIR6257008.1"
                     /translation="MTSILTNSRERTRFLRFAVVGMIGAVIDFGVANLLVYVFFAPLV
                     IAGTISFISAILSNFFWNRYWTYPDSRSKPIAQQLIQFAVVNGLGLTIRIPILRWLEP
                     VLFRLFSRLPFSLPVFSPDVLAKNLTLAVAVLIVMFWNFFVNRYWTYSDVE"
     gene            complement(943..1902)
                     /locus_tag="AC16V7_07535"
     CDS             complement(943..1902)
                     /locus_tag="AC16V7_07535"
                     /EC_number="4.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048040 - UDP-glucuronate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HIR6257009.1"
                     /translation="MRILITGAAGFLGSHLCDRLLAEDHEVIGMDNFITGSPDNLAHL
                     AGNSKFLFIRHDVSNFIYVPGKIDAVLHFASPASPNPNSPYGYVNLPIQTMKAGALGT
                     HNTLGVARSNNARFLLASTSEIYGDPLEHPQTESYWGHVDPIGLRSVYDEAKRFAEAL
                     TMAYHRAHGIDTRIVRIFNTYGPRMHLDDGRVVPNFLQQALRKEPLTVYGDGSQTRSF
                     CYVDDLIDGIVRLLYSNEIQPVNIGNPVETTILEFAHTINRLVDNPAGIVYKPMQRGE
                     GDPQRRQPDISRARQTLGWEPKISIGEGINNTIPYFQSKMGLA"
     gene            complement(1909..3396)
                     /locus_tag="AC16V7_07540"
     CDS             complement(1909..3396)
                     /locus_tag="AC16V7_07540"
                     /inference="COORDINATES: protein motif:HMM:NF019142.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HIR6257010.1"
                     /translation="MSIAADGQNVELQVSAGTTVQQALEAAAITLGGLDRVEPPSYTL
                     LTDGASIRVVRVREEFTIEEEVIPFERQVLQSESLSDQQQLLVQSGVNGLNELTDRKV
                     FENGVEISNTPVKAVIVKDAIPEIIMVGIRAPFTPAEITGRIVYLLGSNAWMMEGSTA
                     NRRPLINSGDLDGRVFSLSEDGRWLLFTRRASEVDDINTLWVADLNADPVDEIDLKVA
                     NVVHFADWVPGSTSKVVFSTVEPRASPPGWQANNDLNALSFSPSGWVSNWQAKPVLET
                     NSGGVYGWWGMNFFWSPNGQRLAFSRPDSVGVLEFKDGVMTTLLDITPLQTGEDWAWV
                     PGVAWGADGNVLYTVSHEAPEDSQLFNLSAIPVESGVPIQLSQESGMFAYPAISPMQS
                     LPSGESAYKVAFLRAVLPRQSKSSSYRLVVMDRDGSNQNILFPPQGEPGLEPQQVVWS
                     PAPLPGDGNYALAILYQGNFWLVNSITGDAQQITGDSLIRRITWR"
     gene            complement(3486..4058)
                     /locus_tag="AC16V7_07545"
     CDS             complement(3486..4058)
                     /locus_tag="AC16V7_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257011.1"
                     /translation="MYSRNYLPVALAVVVLLLVNMACNLTQRSEPTPAEVIPITTEAV
                     ESLKKDLQKAGKEIKLSGQTTLVIDEAELTSLAAFELQNQDSQVFQDPQIFLRDGQIK
                     IATQVEQGNSRASVDIVMEVSADADGRPEYQLVSAKIGPLPLPESMSQQIYKLIDDAF
                     TSKIDPQMDDIFIDNITIKNGLMTVQGHLR"
     gene            4149..4700
                     /gene="scpB"
                     /locus_tag="AC16V7_07550"
     CDS             4149..4700
                     /gene="scpB"
                     /locus_tag="AC16V7_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIR6257012.1"
                     /translation="MSAKLEALLFASPSSVTPSQLAIALEITTQEVEAGLKELELRYT
                     QENFKFGLRLQRLHGRIQLTTAPESAPLIERLLGLEATSRLSRAALEALAIIAYTEPV
                     TRPQIDAIRGVNSDGVLKSLLNKGLIQEIGRAEAPGRPILYSITQDFLGHFGLNSLDD
                     LPPLPPQDGDQENTVSTDELTAL"
BASE COUNT         1266 a         1220 c         1083 g         1155 t
ORIGIN      
        1 catgctcggg ctgcatcaac tgggctatct gaacgcgggt ggagctgacg gttttcccag
       61 gaggagcatc catcttgctc aatccatatc ctcgcgcaaa tcaccgaaat ctactgcggg
      121 aagcatatca ggcaactctt ccaagacgtc attgacacca attggtaact ccggcatcat
      181 tggggccaga ggtatcgtag caggtggatg aatgggcatt ggggatcgcg gtggcgactg
      241 gcgcggcttt agataaccgg atgactgctt gcgaaggatg cgtgggtcat cgctaagata
      301 acccgcgtaa tgtccatgga cagagtagac ctgcctttcc ggcgtcaccc atccaatcca
      361 ttctccttga cgattatata tatacggata cttgagatat ccccccacat ctccacttgt
      421 tgtatagata gcgatcaagt gcaacttgtt agtcatatct attaggaatt atagcatccc
      481 aagatcattc cacatcacta tatgtccaat atcgattgac gaagaaattc caaaacatca
      541 caatgagcac tgcaaccgcc aaggtgagat tttttgccag gacgtctgga ctaaacactg
      601 gcaacgaaaa aggcagcctg gaaaaaaggc ggaataaaac tggctccaac catcgcagaa
      661 tgggtatgcg gatcgtcagc cccaagccat tgaccaccgc aaattgaatt aattgctggg
      721 cgatgggctt agagcgcgag tccggatatg tccagtagcg attccagaag aaattgctta
      781 atatagctga gataaatgag attgtcccgg ctattaccag cggggcgaag aaaacataaa
      841 ccagcagatt cgcaacacca aaatcaatca ctgcaccgat cattccgaca accgcgaagc
      901 gcaagaaacg cgttctttca cgcgagtttg tcagaatgct ggtcatgcta gccccatctt
      961 ggattgaaaa tatggtattg tattatttat cccctcacca atgctaattt ttggttccca
     1021 gcccagcgtc tggcgtgctc ggctgatatc aggttggcgc cgttgcggat cgccttctcc
     1081 acgctgcata ggcttataaa caattcctgc agggttatcc accaagcgat taatggtgtg
     1141 ggcaaattcc agaatggttg tctccaccgg attaccaatg ttaaccggct ggatctcatt
     1201 cgaatatagc agacgaacga tcccatcaat taaatcatcg acataacaaa agctgcgcgt
     1261 ctgcgatcca tccccataaa ctgtcaaagg ctctttgcgt agggcttgct gcaaaaagtt
     1321 cggcaccaca cgaccatcat ccaggtgcat acgcggaccg taggtattga aaatgcgcac
     1381 gatccgcgta tcaataccgt gcgcacgatg atatgccatt gtcaatgcct cagcgaaacg
     1441 tttggcttcg tcataaaccg agcgcagacc aatcggatca acatgtcccc aataggactc
     1501 agtttgcggg tgttctaaag gatcgccata gatctcgctg gtagaagcca acaaaaatcg
     1561 cgcattgttc gaacgcgcca cacccaatgt gttatgcgtt cccagcgcac ctgctttcat
     1621 cgtttggata ggtagattta catatccata gggagagttc gggttagggc tggcgggcga
     1681 agcaaaatgc agcaccgcgt cgattttgcc cggcacgtag atgaaattag aaacatcatg
     1741 gcgaatgaat aagaatttcg aattccccgc aagatgtgcc agattatcag ggctgccggt
     1801 gatgaaattg tccatcccaa tcacttcatg gtcttctgcc agcaggcgat cgcacaaatg
     1861 cgaacccaag aaacccgcgg cacccgtaat taatatacgc ataaggtatt atctccaggt
     1921 aattcgtcta atcaggctgt cgcctgtgat ttgctgagca tccccagtta tcgagttgac
     1981 cagccaaaaa tttccttgat atagaattgc aagggcataa tttccatcgc ccggaagagg
     2041 cgcaggtgac catactacct gctgtggttc cagcccaggc tcaccctgag gcggaaatag
     2101 gatattctgg ttcgaaccat ctcgatccat gaccaccaaa cgataagaac tggacttact
     2161 ctggcgaggg agtactgctc tcaaaaatgc cactttatat gcactctcac cactagggag
     2221 cgattgcatc ggggaaatcg cagggtaagc gaacatgcct gattcttgag atagttggat
     2281 cggtacgcca ctctctacag gaatggcgga gagattaaaa agttgagaat cttctggcgc
     2341 ttcatggcta accgtgtaga gaacatttcc gtccgctccc caagcaacac ctggtaccca
     2401 agcccaatct tctccagtct gcaagggcgt gatatccagc agagtggtca tcactccgtc
     2461 tttaaattcc agcacgccga cactgtcggg gcgagaaaaa gcgagacgct ggccattcgg
     2521 agaccagaaa aaattcatgc cccaccaccc ataaacacca cctgaattcg tttccaatac
     2581 cggttttgcc tgccaattgg aaacccagcc gctgggacta aaactgaggg cgtttaaatc
     2641 attattggct tgccatccag gagggctggc gcgtggctcg acagtagaaa acaccacttt
     2701 cgaggttgat ccaggaaccc aatctgcaaa atgaacgaca tttgctacct tcaggtcgat
     2761 ctcatccact ggatcggcat tcaggtcagc cacccacagc gtgtttatgt catccacttc
     2821 cgaggcacgc cgggtgaaca aaagccagcg gccatcctca gacaacgaaa atactcgtcc
     2881 atctaaatca ccggaattaa ttaatggacg ccggttagct gtactgcctt ccatcatcca
     2941 agcattgctg ccgagaagat acactattct cccggtaatt tcagctggcg taaagggagc
     3001 acgtattccc accatgatga tttctgggat cgcatccttg acaatcactg ctttcaccgg
     3061 cgtattggaa atttctacgc cattctcaaa taccttccgg tcagtgagct cattcaagcc
     3121 attcaccccg ctctggacca gcaattgttg ctggtcggag agcgactcgc tctgcaaaac
     3181 ttggcgctca aacgggatca cttcctcttc aatggtgaac tcctcgcgca ctctcaccac
     3241 ccgtattgat gcaccatccg tcaacagcgt ataagatggt ggctcaacgc gatccagccc
     3301 ccctagggta atcgctgctg cttccagcgc ctgctggaca gtggtacctg ctgaaacctg
     3361 caactccaca ttttgcccat cagcagcgat actcacatta atagttgact gagtagcttg
     3421 aggaggcgaa caggcagcca acacaaggca aagcagaaga attccgattt tgggatggta
     3481 taatctcatc gcaaatgacc ttgtacggtc atgagcccat tcttgattgt aatattatca
     3541 ataaagatgt catccatctg tgggtcaatt ttactcgtaa aggcatcgtc gatcagctta
     3601 taaatctgtt gggacatgga ctctggcagc ggtagtggac caatttttgc cgaaactaac
     3661 tggtattctg gcctgccatc tgcatcggca gatacctcca ttacaatatc aacgcttgct
     3721 cgagaattcc cttgctcaac ctgtgtggca attttgattt gcccatcccg aaggaatatc
     3781 tgtgggtctt ggaacacttg ggaatcttga ttttgaagtt caaaagcagc caatgaggtc
     3841 aattccgctt catcaatgac cagagtagtt tgaccgctca gtttgatctc tttaccagct
     3901 ttctggaggt ccttttttaa actttcaacc gcttcggttg taattggaat aacctctgct
     3961 ggggtgggct ctgatctctg agtcaagttg caagccatgt ttacaagcaa taatacaaca
     4021 accgccagcg caactggcag ataatttctt gaatacatta aattctccat ttcgatctat
     4081 ttcttcaacg ctccggcaca ttttatcata gcatggtaga gacaattgaa cccgaaaatg
     4141 aactgagttt gtccgcaaaa cttgaagcgc ttctatttgc ttccccgtct tcggtgacgc
     4201 catcacaact ggcaattgct ttggagataa ctacccaaga agtggaagcc ggtctaaaag
     4261 agctggaatt gcgctacact caggaaaact ttaagtttgg gttgcgtttg caacgacttc
     4321 acggtcggat ccagcttaca actgcccctg aatcagctcc acttattgaa cgtttattgg
     4381 gactggaagc caccagccgg cttagccggg ctgcattgga agctttggcg attatcgcct
     4441 acacggaacc cgtcacgcgc ccgcaaattg atgcaatccg cggcgtaaat agcgatggcg
     4501 tgctgaaaag cttgctgaac aaagggctaa ttcaggaaat tggccgcgct gaagcccctg
     4561 gaaggccaat cttatacagt attacacagg actttctagg gcattttggc ttgaactcgc
     4621 tagacgacct accaccactc ccgcctcaag atggcgatca ggagaataca gtatccactg
     4681 atgagttgac tgcattataa taatctccag tggaagaacg tttg
//
LOCUS       DBNHOH010000428         3201 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1755067, whole genome shotgun sequence.
ACCESSION   DBNHOH010000428 DBNHOH010000000
VERSION     DBNHOH010000428.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1755067"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1733
                     /locus_tag="AC16V7_07555"
     CDS             <1..1733
                     /locus_tag="AC16V7_07555"
                     /inference="COORDINATES: protein motif:HMM:NF012562.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HIR6257013.1"
                     /translation="QAMIQKDRKDFSAKLGVYQEGVTAALAEMQRDRIIPRIWAHDHT
                     VWKPEPVEIANRLGWLHSPEAMLETIPRLHALAEQLRAEGFRHALLLGMGGSSLAPEI
                     FRKTFGVGEGYLDLAILDSTDPDAVKDWGNRLDLSRTLFIVSTKSGGTVETFSFFKYF
                     YQQLTSHVSKERVGNHFIAITDPGSGLAEIAQKHDFRATFLNDPNIGGRYSALTYFGL
                     VPAALIGADLKTLLERASTAASNCKESIDQNEGVNLGAHLGAILGEMAKAGRDKLTLV
                     TSTPISSFADWVEQLVAESTGKDGTGILPVVGESPGTADMYENDRLFVNLRLAGDSTH
                     DEMLNALKANGHPVVRLELKDAYDLGAQIFYWEMATAVAGYRLGIHPFDQPNVEAAKV
                     QARKMVSAYHSEGKLPEPTPDLQLGEITIYLDQKSRIVGNNPGEILNAFLHQAQPGDY
                     VAMQAYVQPGPEMDEMLGKLRERIRSHTQLATSIGYGPRFLHSTGQLHKGDAGKGLFI
                     QFTSQVQGDVAIPDEAGFPDSSMSFGVLKLAQALGDRQALLDNRRRIIRIHLGERPVA
                     SLETILKQLT"
     gene            1812..3116
                     /locus_tag="AC16V7_07560"
     CDS             1812..3116
                     /locus_tag="AC16V7_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257014.1"
                     /translation="MEKIFPSQHTSAWQWIALVLWTLFALAFLAFFLIDSRLDYIQLL
                     APCKGTDCNWMAISSAEVELLESWGLYTRAYAALMIGTAALTVAVYWLLGGLVLLRQG
                     AARFGLAVSLVLLSIPIAMISDPDNLYASYPALLLPSIFLQSLGTIFLLLFLYLFPNG
                     RFYPRLASIPLAGAVLIILISDVLEVSGFDLDSPVQITLFQALFMMLALGTVFQIFRY
                     RRDSTLLERQQTKWVLFGFSILTLSFPLWGSIFGGFLEIPPGEARLLANILGWITIQF
                     ILIALPISIAIAILRYRLWDIDFIIRRTLIYGGLTLTLAAVYFGSVVLLQALSQRVVG
                     QESTVAIVLSTILIAALFNPLHKRIQSDIDQRFYRKKYDTQRTLETFAASLREEVELE
                     QLTGQLQAVVQETMQPEHVSLWLRTPDVNEAINLQPPKASVD"
BASE COUNT          748 a          857 c          809 g          787 t
ORIGIN      
        1 aacaagccat gatacaaaag gatcgcaaag atttttctgc caaactgggg gtgtaccagg
       61 aaggcgtgac agcagcgtta gccgagatgc agcgcgatcg catcatcccc cgcatatggg
      121 cgcatgacca cacggtctgg aaacctgagc cagtcgagat cgccaaccgg ctaggctggc
      181 tacacagccc agaagccatg ctggaaacca tccctcgctt gcatgccctg gcggagcaac
      241 tgcgagctga gggctttcgc catgcactgc tcttaggtat gggcggttcc agcttagcgc
      301 cagaaatttt ccgcaagact ttcggtgtag gagagggata tcttgatctg gccattttgg
      361 acagcactga tccagatgct gtgaaggact gggggaaccg ccttgacctg agccgaaccc
      421 tgttcatcgt atcgactaag tccggcggga cggtagagac tttctctttc tttaagtatt
      481 tctaccagca actgacgtcg catgtaagca aagaacgggt tggcaatcac ttcatcgcca
      541 taactgatcc aggcagcgga ctggctgaga ttgcccagaa acatgatttc cgcgccacct
      601 ttttaaatga tccaaacatc ggtggtcggt attctgccct gacctacttc ggcctcgtcc
      661 cagcggcatt gatcggtgca gatctcaaaa cactactgga gcgtgcgtca accgctgcca
      721 gcaactgcaa ggagagcatc gaccagaacg aaggagtcaa tcttggagca cacctggggg
      781 caatattggg cgagatggcg aaagccggcc gggataagct caccctggtg acgtctaccc
      841 caatctccag ctttgcagat tgggtagagc aattggtcgc cgaaagcacc ggaaaagacg
      901 gtacgggcat cctccccgtg gttggtgaat ctccgggcac cgcggatatg tatgaaaatg
      961 accgcctatt cgttaatctg cgattggctg gagactccac gcatgatgag atgttaaacg
     1021 cgcttaaagc gaatggtcat ccagtcgtgc gactcgaatt gaaagacgcc tatgatttag
     1081 gcgctcagat cttctattgg gagatggcta cggcggtggc cggctacagg cttgggattc
     1141 acccctttga ccagccaaat gtcgaggcgg ctaaggtcca agcacgcaag atggtctcgg
     1201 cttaccattc ggaggggaaa ctgcctgaac ctaccccaga tttacagctt ggggaaatca
     1261 caatttacct ggatcaaaag agtcgcatcg tgggaaataa tcctggtgaa attctgaacg
     1321 cgtttttgca tcaagctcag ccaggtgact atgtcgccat gcaggcttat gtgcaacccg
     1381 gtcctgagat ggatgaaatg cttggcaagc tgcgcgagcg aattcgctct catacccaat
     1441 tagcgaccag cattggttac ggtccgcgct tcttgcactc cactggccag cttcacaaag
     1501 gcgatgctgg gaagggtttg ttcatccagt ttacctctca ggtacaggga gatgtcgcta
     1561 tccccgacga ggccggtttc ccagactcgt cgatgagttt tggagtgtta aagttggcac
     1621 aggccctcgg tgaccggcag gcattgttgg acaatagacg ccgtattatt cgcatccatt
     1681 tgggcgaaag gccagtcgcc agtctggaaa ccatcttgaa acaactgaca tgattcatcc
     1741 agtctcccct tagtggttga gagcatgaac aaggagcaca ataaccgggt tcaccagaat
     1801 agtgaggatt aatggaaaaa atatttccgt cacaacatac ctctgcttgg caatggatcg
     1861 cgctggtatt atggacatta tttgcgctgg cattcctcgc ctttttcctg atcgattcgc
     1921 gcttggatta catccagctt ctggcgcctt gcaagggcac agattgcaac tggatggcaa
     1981 tttcatcagc cgaggttgag cttctcgagt cctgggggct atacacccgg gcttatgctg
     2041 ctttgatgat agggaccgcc gctttgaccg tggctgttta ctggctattg ggcgggttgg
     2101 tcctgttgcg acagggagcc gcccgatttg ggctagcggt ttcccttgtt ttactgtcca
     2161 tccccattgc gatgatttca gaccctgata atctatacgc cagctatccg gctctgcttt
     2221 tgccttcgat ttttctccaa tcccttggca cgatcttcct gctgctgttc ctttacctct
     2281 tccctaacgg acgtttttac ccccgcttgg catccatccc actggcagga gccgtcttaa
     2341 tcattttgat cagcgatgtc ttagaagtca gtggattcga ccttgattca ccggtacaaa
     2401 ttacattatt ccaagcctta ttcatgatgc tagccctggg tactgtcttt caaatatttc
     2461 gctacagaag agattccacg ctcctagaac gccagcaaac caagtgggtc ttgttcgggt
     2521 tttctatcct cactctcagt tttccactct ggggatccat tttcggtgga ttcctagaaa
     2581 taccccctgg agaggccagg ttgctggcaa acatacttgg ctggatcaca atccaattca
     2641 tcttgatagc cctgccaatt tcgatcgcca tcgccatcct gcgctaccgg ttgtgggata
     2701 ttgactttat catccgccgc acattaattt atggtggtct cacgctcacc ttggccgcgg
     2761 tctacttcgg tagcgttgtc ttgctacagg ctctctccca acgcgttgtc ggccaagagt
     2821 caactgtggc gatcgtcctt tctactatct tgatcgccgc cctgttcaac ccactacaca
     2881 agcgcatcca gagcgacatc gaccagcgct tctatcgaaa gaaatacgac acacaacgca
     2941 cgctggagac tttcgctgcc agcctgcgcg aggaggtcga gctggaacaa ttgaccggac
     3001 aactccaggc agtcgtgcag gaaaccatgc agccggagca cgtcagcctc tggttgcgaa
     3061 cgcctgatgt aaatgaagcg attaacctcc aaccacctaa agccagcgtg gattaaccag
     3121 aagattgaaa ttcttcgtat acaaggagat cgcatgtcac tcactcatat gcaaataaaa
     3181 gacttattgg atgaaaaccg c
//
LOCUS       DBNHOH010000429         5252 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1050593, whole genome shotgun sequence.
ACCESSION   DBNHOH010000429 DBNHOH010000000
VERSION     DBNHOH010000429.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5252)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1050593"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="AC16V7_07565"
     CDS             complement(<1..356)
                     /locus_tag="AC16V7_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase"
                     /protein_id="HIR6257015.1"
                     /translation="MLRSFLIYLSQAAWARKIVTSWSIAWKAASRFIAGETLNDAINV
                     VKKLNEKGINATLDHLGEHTTNPDEARRATQDILRILDAIQQTGVRSYVSIKLTQIGL
                     ALDESLCEENLMTILER"
     gene            674..2089
                     /locus_tag="AC16V7_07570"
     CDS             674..2089
                     /locus_tag="AC16V7_07570"
                     /inference="COORDINATES: protein motif:HMM:NF016827.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HIR6257016.1"
                     /translation="MLQQSQAPITRPITTAHLATTMSLMSLTAIELHERIEAELASNP
                     ALELGEKHHCTSCGRPLVEKGTCLVCSRLSPGLGEEPIVFISPREDFYLPARQSTPNG
                     IPGDEITAETEDLPTYVLRQIAPELAPEDRLLAAHLLTSLDEDGLLRVPLIEFARYHH
                     RPPSHIEQLLKLIQRADPIGVGSSSPEEAMLVQLEVLGETQSIPPMTDQVIRQGIGLL
                     SRRHFTELGKLLGISASQVKQIARFISGNLNPYPARSHWGNVHQGAEPAQDVYHFPDI
                     LISRLNDNPDSSLIVEVISPLAGRLRLNLLFRQSIRLAPEDKVEEWQNDLEQASLLIK
                     CLQQRNHTIVRLMQRIAAIQRHFILHGDAYLEPLTRASLAEELQVHESTISRAVAGKA
                     VQLPNGKIVPLSKMFDRSLHIRTELRQIIAHECQPLTDTELAALLKKKGYRVARRTVA
                     KYRAMEGILPAHLRQPLQTAG"
     gene            2097..4067
                     /locus_tag="AC16V7_07575"
     CDS             2097..4067
                     /locus_tag="AC16V7_07575"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HIR6257017.1"
                     /translation="MNTPLKLNMLPGLRPTPGLAEIESMMELFKSACLLVESRNRRIL
                     LANSQATELTAYTRGELSGMAFSKLIDQPNKHPFWESPSGQPFPTALTLLRRNGAKVS
                     VQATRHELAAQSKWAIIQLEESHVIEQRQSQQRRQSGMIQSMVTISQAIGLPDLSEAI
                     TMILEASHEITGCDNIGVYLQEMATKGQGFEMVRYAQHGADNILPERLPAQDLVHLRA
                     PNYWIPGKRSASSLHRAARSAGLAFLASVPLGQDKAPVGFIAIAGGAPIAQNEILPPL
                     EILANIIDVLIQIHTRTTNLEKSLGDNIHARALEKTSMDAIEDGIIVLTPTLNVVRLN
                     PAAETILGYTNREARNRPVEDILIGSGALTTSLELALKGVPTIKQENPHLYRRSGTPF
                     LAQVSIIPAHVEDHLEGIVVLIRDLSEQEQIQVQAEQLKQRAVLGEVSSIFAHEVRNP
                     INNISTGLQVMAYNLSPDDPHQEVISRLQRDCERLDELMKSVLTIGRPAEYKLGPTDL
                     SMLATRLVERFKPRMVNANIQPFLQIEPSLPLVNGNLLALDQVFINLINNAIEAMSEK
                     GGTLALRLQTATDGDGKRFVEVNVADNGPGIPKEVQERLFQPFFTTKKDGNGLGLVIT
                     RRILNAHKGTITLTSYPGATIFHVQIPVLESP"
     gene            4064..5239
                     /locus_tag="AC16V7_07580"
     CDS             4064..5239
                     /locus_tag="AC16V7_07580"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR6257018.1"
                     /translation="MSLTVLIVDDEENARYNISSFLGAHGYEVLGVSTLSEARETLRR
                     GNADIILLDVTLPDGFGTDLLEETAHMPNRPPIIMITAYGNIEMAVNAMKNGAHDFLP
                     KPIQLAELEKSIQRGGELVALRRELAHLRSVQHNQVDFVVGQTPVMRNLLNQAQRAAA
                     ASVSVLITGETGTGKEVLARAIHGMGPRSSKPFIGENFAAIQPTVLESELFGYEPGAF
                     TGAEKRKIGLMEVAEGGILFLDEISSLSVDMQAKLLRALEERAFRRVGGNNQIRVDVQ
                     FLAASNRHLPTLITEGKFREDLYYRLKVVDLHLPPLRERKEDIPELVGLFIRQLNPRM
                     GLNITDVTPRALQALMSHNWPGNIRELHNIIERAMLFCDEAAIDLPHLPAELSNGKE"
BASE COUNT         1285 a         1522 c         1301 g         1144 t
ORIGIN      
        1 cgctccaaga tggtcatcaa gttctcctcg cacaggcttt cgtccagcgc aaggccaatt
       61 tgtgtcagtt taatcgacac ataggagcgc acaccagttt gctggattgc gtctaaaatc
      121 ctcaggatgt cttgtgtcgc ccggcgagcc tcgtcgggat tggtggtgtg ttcgcccaga
      181 tgatccagcg tggcgttgat ccctttttca tttaactttt ttaccacatt gatcgcatca
      241 ttcagagtct cccctgcgat gaagcgtgag gcggccttcc aagcaatact ccagcttgtg
      301 acaattttcc gcgcccaagc ggcttgtgag agataaatga ggaaggagcg aagcatatct
      361 gaataccctc atgttgctgt taatgatacg aaacccaacc cctcggggtc ggcaatgtca
      421 gtctcatgca gaaatttgat caagcgaatc cattgtagca taaatcactt ttgaagtctg
      481 tttacaagca tcctgcctcc agccgacaaa cttcacatta aaggtcaact ctgatcaagc
      541 aaatgaggga gatttgtgcg aagcaagccg tctaataccc taatccgtgt tcagctttac
      601 tgcttgcatt cacctatccc tggcacattt cttgcaacag gttataatca gatattgcac
      661 aaaaggaaaa aacatgctgc aacagtccca ggctcctatc acccgcccca taaccaccgc
      721 tcacctggcg acgacgatgt cgttgatgag cttaaccgcc atcgagctgc acgagcgaat
      781 cgaggcggag ctagcctcca atccggcctt ggagctgggt gaaaaacatc actgcacttc
      841 ctgtgggcgt cccctggttg agaaaggaac ctgcctggtg tgcagccggc tctcaccagg
      901 gttaggggaa gaacccatcg tgttcatctc cccgcgcgaa gacttctatc tcccggcacg
      961 ccaaagcaca cccaatggaa tacccggcga cgagatcacc gccgagacag aagatttgcc
     1021 cacctatgtc ctgcgtcaga tcgcccctga gctagccccc gaagaccgcc tgctggcagc
     1081 ccacttgctc accagcctgg atgaagacgg gctactgcgc gtccccctga tcgagttcgc
     1141 ccgctaccat caccgaccgc cttcccatat tgagcaactt ttaaagctga tccagcgcgc
     1201 tgacccgata ggcgttggca gctcatctcc cgaagaagca atgctggtgc aacttgaagt
     1261 cctgggagaa acgcagtcca tcccccctat gaccgaccag gtcatccgcc agggcatagg
     1321 gttgctctca cgccgccact tcacggagtt gggcaaattg ctgggcatct ctgcctccca
     1381 ggtcaaacaa atcgctcgct tcatcagcgg taaccttaac ccctatcctg cccgctccca
     1441 ctggggtaat gtccatcaag gtgccgaacc ggcacaggac gtatatcact tccccgatat
     1501 ccttatcagc cggctgaacg acaaccccga ctcgtccctc atcgtcgaag tgatctcccc
     1561 tttggcaggc aggttgcggc taaacttact attccgacaa tcgatccgtc tcgcgcctga
     1621 agacaaggtc gaggagtggc agaacgactt ggagcaggca tccttactga tcaaatgcct
     1681 gcaacaacgc aatcacacca tcgtacgctt gatgcagcga atcgcggcca tccagcgtca
     1741 ttttatctta cacggtgacg cttacctgga gccgctgaca cgcgccagcc tggctgaaga
     1801 actccaggtg cacgaatcca ctatctcccg cgcggtagcc ggaaaagcgg tacaattgcc
     1861 caatggcaag atcgtgccac tctcgaagat gttcgaccgc agtctgcaca tccgcaccga
     1921 actgcgccag atcatcgccc atgagtgcca accactcacg gatacggagc ttgcggcatt
     1981 attaaagaaa aaaggctacc gggtggctcg ccgcaccgta gcaaaatacc gtgctatgga
     2041 gggcattctg ccggctcact tacggcaacc cctgcagacc gctggctagt ggattgatga
     2101 ataccccact caaactcaac atgctccccg gattgcgccc cacaccagga ctggccgaga
     2161 ttgagagcat gatggagcta ttcaaatccg cctgtctatt ggtggaatct cgcaatcgac
     2221 gcatcctgct ggccaattcc caggccacag agctgaccgc ctacactcga ggcgagttgt
     2281 ctgggatggc tttttccaag ctgattgacc aacccaataa gcatcctttc tgggagtccc
     2341 cctctggaca accctttcct actgcgctaa ctctgcttag acgtaatggg gcaaaagtta
     2401 gcgtgcaagc gacgcgacac gaactggcag cacaaagcaa atgggccatc atccaattgg
     2461 aagaatcgca tgtaatcgag caacgccaga gccagcagcg ccgacagtcg gggatgatac
     2521 agagcatggt taccatcagt caggccattg ggctgcctga tctaagcgaa gccatcacaa
     2581 tgattttaga agcaagccat gagattaccg gttgcgacaa catcggtgtc tatcttcaag
     2641 aaatggccac caaagggcaa ggctttgaga tggttcgcta tgcccagcat ggcgcagata
     2701 atatcctacc tgagcgctta cctgcccaag atctggtgca tctacgtgcc cccaattact
     2761 ggattcctgg gaagcgttct gcctccagcc tacaccgggc ggctcgcagc gctgggttgg
     2821 cctttctggc ttctgttcca ttgggacagg ataaagcccc ggttggcttt attgccatag
     2881 ccgggggtgc cccgatagca caaaatgaaa tcttgcctcc cttggagatc ctagcgaata
     2941 ttatcgatgt gctgatccaa attcacacac gcaccacaaa tttagaaaaa tctcttggcg
     3001 acaacattca cgctcgcgca ttagagaaga ccagcatgga tgccattgaa gacggcataa
     3061 ttgtactcac accgacactc aatgtggtgc ggctgaatcc tgctgccgag acaatcctgg
     3121 gatacacaaa ccgggaggcg cgtaaccggc cagtcgaaga cattctcatc ggttctggtg
     3181 cgttaaccac ctctctggag ctcgccctga aaggcgtacc caccatcaaa caagaaaatc
     3241 cacacctcta ccgtcgttct gggacaccct tccttgccca ggttagcatc ataccagcac
     3301 atgtggaaga tcatttagag ggcatcgtgg tcctgatacg ggacttgagc gaacaggagc
     3361 aaatccaggt ccaggcagag caactcaaac agcgtgctgt gcttggcgaa gtatcctcaa
     3421 tcttcgccca cgaggtgcgc aatcctatca acaacattag cactggtctg caagtcatgg
     3481 cctacaactt atcccccgac gatcctcatc aggaggtgat ctcccgcttg cagcgcgatt
     3541 gtgagcgcct ggatgaatta atgaaatctg tattgaccat cggtcgccca gcggaataca
     3601 aattgggtcc aactgacctg agcatgctgg caactcgtct ggtggagcgt tttaagccac
     3661 gcatggtgaa cgccaatatt caaccattcc ttcagattga gccttccttg cctctggtga
     3721 atggcaactt gctcgccttg gaccaggtct tcatcaacct gatcaataac gcgattgagg
     3781 cgatgagcga aaaagggggc accctggcat tgagattgca gaccgccaca gatggtgatg
     3841 gcaaacgctt cgtagaagtc aatgtagctg acaacggtcc tggcattcct aaagaggtac
     3901 aggaacgcct tttccaacct ttcttcacca ccaaaaaaga tggcaatggc ctaggtctgg
     3961 tgatcacccg ccgcatccta aatgcccata aggggacaat caccctcacc agctacccag
     4021 gcgctaccat atttcacgta caaattcccg ttctggagtc gccatgagct tgaccgttct
     4081 gattgtagat gatgaagaaa atgcccgcta taacatcagc tcgttcctgg gagcccacgg
     4141 gtacgaggtg ttgggtgtat ccacattaag tgaggcccgc gagacgttgc gccgcggtaa
     4201 tgcggatatc attttgctcg atgtaacact tcctgatgga ttcggcaccg acctacttga
     4261 agaaaccgca catatgccca atcgcccccc gattattatg atcacggcgt atggcaatat
     4321 cgagatggcg gtaaatgcca tgaaaaacgg cgcgcacgat ttcttaccca agccaatcca
     4381 gcttgctgaa ctggagaagt caatccagcg cggcggggaa ctcgttgcct tgcgcagaga
     4441 gttggcacac ctgagatcgg ttcagcacaa ccaggttgat tttgtcgttg ggcagacgcc
     4501 ggtgatgcga aacctactta accaggcaca acgcgccgcg gctgcatcgg tctcggtcct
     4561 gatcaccgga gagacaggca caggcaaaga agtattggcg cgcgccatcc acggcatggg
     4621 gccacgctcc agcaagccct ttatcggtga aaactttgcc gccatccaac ctacggtttt
     4681 ggaatcggag ttgtttggct acgagccagg ggctttcacg ggcgcggaga agcgcaagat
     4741 tggcctgatg gaagtggcag aaggtggcat cctgttcctg gatgaaatct cctccttatc
     4801 agtggatatg caagccaagc tgctgcgcgc cctagaggag cgcgccttca gacgggttgg
     4861 cggcaacaac cagattcgtg tggatgtgca attcctggca gcctccaacc gtcacctgcc
     4921 cacactgatt accgaaggca agttccgcga ggatctttac tatcgcttga aggtggtaga
     4981 cctgcacctg ccaccgttgc gcgagcgcaa ggaggacatt cccgagctgg taggattgtt
     5041 tatacgccag ctcaacccac gtatgggact taatatcacg gacgtcacac cgcgcgccct
     5101 acaggctctt atgtctcata attggcctgg aaatatccgc gaacttcaca acatcattga
     5161 gcgagccatg cttttctgtg atgaagcagc catcgatttg ccccacctgc ctgccgagtt
     5221 atcgaacggt aaagaataat ttgcgcagtc ga
//
LOCUS       DBNHOH010000430         2614 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_408689, whole genome shotgun sequence.
ACCESSION   DBNHOH010000430 DBNHOH010000000
VERSION     DBNHOH010000430.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_408689"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="AC16V7_07585"
     CDS             <1..410
                     /locus_tag="AC16V7_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012966992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase C-terminal domain-containing protein"
                     /protein_id="HIR6257019.1"
                     /translation="WGQLPYLQKAARMAYQQAGIRSPIGVVDFAEVDDLYAYKELQSL
                     EALGLCGIGEAGSITEEGLTEADGELPVNVSGGSLGIGNLLDATGLSRALEVVLQLRG
                     EAGARQLENAEIGLAQSWRGVPTTSAAVAVFSN"
     gene            423..1589
                     /locus_tag="AC16V7_07590"
     CDS             423..1589
                     /locus_tag="AC16V7_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014512167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HIR6257020.1"
                     /translation="MGMRKVAVIGAGMTKFVRRAQETAKELSWEAARMALETSELTLD
                     DVDAVCMGTAPDAFDSVHMNGEYLSDGAGAWRKPYMRSYVGGGTGVFAPIQGWYHVAS
                     GLFDVVLVVAEEKMSSFYPHAQAAFLTIFDHTTERPLKPNLLWIFALEMNRYMQTYGI
                     SKDDIARVSVQHKRNAADHPCALLGDADITVQDVLNSEVLAWPVQRLDVSPVSDGAVA
                     IILAAEHIANRVTDKPVWIEGVGWSLDTAYWANRDLAYPRYVESAARMAYDMAGIREP
                     RKQINIVEPYDPFDYKALHHLEGLQLADRGQAPQLYIDGIAARDGNMPVCPSGGLLGV
                     GNPIAAAGLMKIAELYWQLRGEAGNRQIPGSPRRGLAQAWGDLMQVGTVVIMGR"
     gene            1609..2124
                     /locus_tag="AC16V7_07595"
     CDS             1609..2124
                     /locus_tag="AC16V7_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010878466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR6257021.1"
                     /translation="MTAKITNFPGTPLGEEDFAEGKVLFNYDQMRGDFAWDTGIAIGR
                     YLAALKEGIILGARCATCRKTVVPPRTVCEWCFRPMQEYVPLQDSGIVNTFSLCYVTW
                     DVQRIKEPEIPAVIEIDGASPLHGIMHKIGGVDPQKVHIGMRVKAVWRPPSEREGAIT
                     DIMYFKPIEEV"
     gene            2126..2593
                     /locus_tag="AC16V7_07600"
     CDS             2126..2593
                     /locus_tag="AC16V7_07600"
                     /inference="COORDINATES: protein motif:HMM:NF013920.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR6257022.1"
                     /translation="MSILERDVNAPASWNGNLPVTSRYTFGLAGERFFRAIKDEGRLY
                     GTHCPNCNRTYVPAASFCERCLSELTEWIDVGLVGEVHTFTLLHENYDGSPRETPEVV
                     AFIRMEDGGLVHRIDEIQPDEIEIGLHVEAVFKPKVERNGSILDILYFKPANL"
BASE COUNT          621 a          655 c          721 g          617 t
ORIGIN      
        1 agtggggaca gctcccatac ctacaaaaag ccgcacgaat ggcttaccag caagcgggca
       61 ttcgcagccc aatcggagtt gtagattttg ccgaggtgga cgacctctat gcctacaaag
      121 agctgcaatc gcttgaagcc ttgggtttat gcggaatagg tgaggcgggc tcaatcaccg
      181 aggaagggtt gaccgaagcg gacggagagc tcccggtgaa cgtatccggc gggagtttgg
      241 gtataggaaa tctgctcgat gcaaccggtc tctcgcgagc cttggaggtc gtcttacaat
      301 tgcgaggtga agctggcgct cggcaactcg agaacgccga aatcggcctc gcccagtcct
      361 ggcgcggcgt tcccaccacc agcgctgcag tcgctgtatt tagcaactga aaggaggtaa
      421 tcatggggat gcgtaaagtt gccgtgattg gcgccggcat gaccaaattt gtacgccgtg
      481 cccaggagac cgccaaagaa ctttcctggg aagcggcgcg tatggcgctc gaaactagtg
      541 aattaacctt agacgatgtt gacgcggttt gcatgggaac tgcccccgac gcatttgaca
      601 gcgtgcacat gaatggggaa tatctcagtg atggtgctgg cgcttggcga aagccctata
      661 tgcgctccta cgtaggcggt ggaacagggg tatttgcacc catccagggt tggtaccacg
      721 ttgcatccgg gttatttgat gtcgttctgg tcgtggcaga agagaaaatg tcaagctttt
      781 acccacacgc ccaagccgct ttcttaacca tctttgacca cactactgaa cgcccgctca
      841 aacccaacct gctgtggatt tttgctctcg aaatgaaccg atatatgcaa acctacggca
      901 tctctaaaga cgatatcgct cgagtttccg ttcaacacaa gcgtaacgcc gctgatcatc
      961 catgtgcgct gctaggtgat gccgacatca ctgtccagga cgtactcaac tcagaagtgc
     1021 tagcctggcc agtccaacgc ctagacgtca gccctgtaag tgatggagct gtggcgatta
     1081 tattggcggc tgagcatatc gccaatcgag tcacggataa gccagtctgg atcgaaggcg
     1141 taggttggtc cctggatacc gcttactggg caaaccgcga tcttgcctat ccacgctatg
     1201 tcgaatctgc tgcgcgtatg gcttatgata tggcaggcat ccgcgagccg cgtaaacaaa
     1261 ttaatatcgt tgagccttat gatccattcg actacaaagc cctccaccac ttagaaggct
     1321 tgcagctagc agatcgaggg caggcacctc agttatatat cgacggtatc gctgcccgcg
     1381 atgggaatat gcccgtttgc ccatcgggtg gtctcttagg cgttggcaat ccaatcgctg
     1441 cggctggcct aatgaagatt gctgaactct actggcagtt acgtggtgag gctggcaatc
     1501 gccagattcc tggatcgccc cgcagaggcc ttgctcaagc ctggggtgac ctgatgcagg
     1561 ttggcacggt cgtgatcatg ggacgttaaa ttttggcgga gaatgaatat gactgcgaaa
     1621 ataactaatt ttcctggtac acccctgggt gaagaagatt tcgccgaggg aaaggtgttg
     1681 ttcaattacg atcagatgcg cggcgacttc gcctgggata ctggtatcgc catcggtcgc
     1741 tatctggcag ctctgaaaga agggattatt ttgggagcgc gctgcgctac ctgtcggaaa
     1801 accgtcgttc caccccgcac ggtttgcgaa tggtgttttc ggcctatgca agaatatgta
     1861 ccccttcagg attcgggcat tgtcaacact ttttcgcttt gttacgtcac ttgggatgtg
     1921 caaagaataa aagagcctga aatacccgcc gttatagaga ttgatggcgc atcccctctg
     1981 catggcataa tgcataaaat tggaggcgtc gaccctcaaa aagttcatat tggcatgcgt
     2041 gtgaaggcag tctggcgtcc ccctagtgag cgcgagggag caatcaccga cataatgtac
     2101 ttcaaaccaa ttgaggaggt gtaatatgag catcctcgaa cgtgatgtta acgctcccgc
     2161 ttcctggaat gggaatctac cggttaccag tcgttacacc tttggccttg caggtgagcg
     2221 atttttccga gcgatcaaag atgagggacg tttgtatggc acgcattgcc cgaactgcaa
     2281 tcgcacatat gtgccagcag cttccttctg tgagcgctgt ctaagtgagc tcaccgaatg
     2341 gatcgatgtc ggtcttgtgg gcgaggttca caccttcacg ctcctccatg aaaactacga
     2401 cgggagcccc cgagagaccc cagaggttgt cgccttcatc cgcatggaag atggcggatt
     2461 ggtacaccga atagatgaga ttcaacctga tgagattgag attggcctgc acgtagaggc
     2521 agtattcaaa cctaaggttg agcggaatgg ttctatattg gatattctat atttcaagcc
     2581 agccaattta taagcaaaac attgattgac gcgg
//
LOCUS       DBNHOH010000431         3130 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_408736, whole genome shotgun sequence.
ACCESSION   DBNHOH010000431 DBNHOH010000000
VERSION     DBNHOH010000431.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_408736"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="AC16V7_07605"
     CDS             <1..398
                     /locus_tag="AC16V7_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="BMP family lipoprotein"
                     /protein_id="HIR6257023.1"
                     /translation="PYDFEGSCEQAPDICLGVPYFNWGPAYLETVKAVQAGTWEQSWD
                     WNPPAWDALTDNTKTAVGWLDGSALTSEMKVSLDEFIAGLASGDINVWTGPVTLQDGT
                     EYVAAGAVATDDEIWYLPQLLDGMEGPSE"
     gene            501..2069
                     /locus_tag="AC16V7_07610"
     CDS             501..2069
                     /locus_tag="AC16V7_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014904362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6257024.1"
                     /translation="MMSDTDTLPSGHPRITHLELRGIVKRFPGVLANDHVNFDVRAGE
                     VHALLGENGAGKSTLMKILYGLYQPNEGEIWLNGSKVTISSPSDAIRLGIGMIHQHFM
                     LVPSLTVAENVALGLPSSRGPITDLDLVSIRINELAKVYGLQVDPSTYVWQLSVGQQQ
                     RVEIIKSLYRGAALLILDEPTAVLTPQEVDDLFVTLNQMAKDGHAMIFISHKLHEVLS
                     ISDRVTVLRDGRVVGTQPTSSTTKGELANMMVGRPVSLKIDHQQVELGQVRLELNDIW
                     AMSDRGTEALRGVNLEVRSGEILGLAGVSGNGQRELAEVIAGLRPATRGKVMLDSQVV
                     SGRSPGELIEYGLSYIPEERMRDGMIREFTVSENLILREHHKMPYSQAGFLRPPDITK
                     ISAELIEDFNVKTPSQETPVKNLSGGNIQKLILARELSRKPGVVIAAQPTRGLDIAAT
                     EYVHYRLMEQRREGTATLLISEDLDEILALSDRIAVIYEGKIMGVVPAAQAEPKELGL
                     MMAGVMAEAKGQEN"
     gene            2174..>3130
                     /locus_tag="AC16V7_07615"
     CDS             2174..>3130
                     /locus_tag="AC16V7_07615"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR6257025.1"
                     /translation="MKKKFLGYQCSLCNTQYAPDEVTYTCPKDGGNLDVILDYEAIKR
                     ETTPEKIAAGEESSLWRYLPLLPVADPQGLGTPLRAAGGTPLFAPKQLAENLGLRSLW
                     VKDESRNPTASFKDRASAVVVARAREVGAEVVVTASTGNAGAALAGMAAAVGQQAVIF
                     APKTAPPAKIAQLLIYGARVLLVDGTYDDAFDLTIQAAQEFGWYCRNTGYNPFTAEGK
                     KTAALEIWEWVKIPRATIFVSVGDGNIISGIHKGFKDLLELGWMENMPRIFGVQAEGS
                     AAVANAFHARTETITPVSARTLADSISVDLPRDGVRAVRAARD"
BASE COUNT          737 a          777 c          916 g          700 t
ORIGIN      
        1 ttccctacga cttcgaaggt tcctgcgaac aagcaccgga tatctgcctt ggcgtgccgt
       61 acttcaactg gggtcctgct tacctggaga ccgtcaaggc tgtccaggca ggcacctggg
      121 aacaatcctg ggattggaat ccgcctgcat gggatgcctt aaccgacaac accaagaccg
      181 cagtgggatg gcttgacggc tcggcgctca ccagcgagat gaaggtgagc ctggatgaat
      241 tcatcgcagg cctggcgtcc ggcgatatca atgtctggac ggggccggtc acgctccagg
      301 acggcacaga atatgtcgca gccggagctg tggcaaccga tgatgaaatt tggtacctgc
      361 cccagctgct ggatggtatg gaaggtccga gcgagtaatc tcgaaccctg tactttggta
      421 gcttaaccat tggagagagc gattatgatg atcgctctct ccttcaccca atagagtcac
      481 aatagagaaa aaaccaaaat atgatgagtg atacagatac tttgccctcc ggtcatcccc
      541 gcataacaca cttagagttg cgcggcattg taaagcgctt cccaggcgtg ctggcaaacg
      601 accatgtaaa ttttgacgtc cgcgccgggg aagttcacgc actgcttggt gagaatggag
      661 cgggcaaaag taccctgatg aaaatcctct atggccttta ccagcctaat gagggggaga
      721 tctggcttaa tggcagcaag gtgaccatct cctcgccttc agatgccatc cgcctgggaa
      781 ttgggatgat ccaccagcac tttatgttgg ttccctccct gacggttgcg gaaaatgtcg
      841 ccttgggttt accatcctcg cggggcccga tcacggatct cgatcttgtc tcgatcagga
      901 tcaacgagct ggccaaagta tacggtttgc aagtcgatcc aagcacttat gtttggcagc
      961 tttcggtggg gcagcagcag cgagtggaga tcatcaagtc cctttatcgg ggcgcagcgc
     1021 tgctcatcct ggatgagccc accgcggtgc tcaccccgca ggaagtcgac gatttgttcg
     1081 tcaccctgaa ccagatggct aaagacggtc atgcaatgat ctttatttct cataaattgc
     1141 acgaagtttt aagtattagt gaccgggtca ctgtgttgcg agatgggcgg gttgtgggca
     1201 cacaacccac atcctctacc acgaaaggtg agctagccaa tatgatggtc gggcgacctg
     1261 tcagcctgaa aatcgatcat cagcaggttg agcttggaca ggtgcgcttg gagctaaacg
     1321 atatctgggc gatgagcgat cggggaacgg aggcactcag aggggtcaat cttgaagtgc
     1381 gatcaggcga aatcctcggg ctggctgggg tatctggaaa tggtcagagg gagctggcgg
     1441 aggtcattgc aggcttgcgc ccagcaacac gtgggaaggt gatgctagat agccaggttg
     1501 tatccggtcg atcgccgggc gagttgattg agtacggttt atcatatatc cctgaggagc
     1561 gcatgcggga tggaatgatc cgggagttca ccgtctctga gaatctgata ttgcgagaac
     1621 accataaaat gccttactcg caggcgggtt tcttgaggcc gccggatatt acgaaaatct
     1681 ctgctgagct gatcgaagat ttcaatgtta aaaccccttc ccaggagacc ccggtcaaga
     1741 atctatctgg cggtaacata caaaagctta tcttagccag agagctttcc agaaagcccg
     1801 gcgtagtcat cgctgcccag ccaacgcgag gattggatat cgcagctacg gaatatgtgc
     1861 actaccggct gatggagcag cgcagagaag gcaccgcaac gttattaata tctgaggatt
     1921 tggatgagat cctggcgctc tctgatcgaa tcgcggttat ctatgaaggc aagattatgg
     1981 gagttgtacc tgcagcacag gctgagccca aagagctggg cttgatgatg gctggggtga
     2041 tggcagaagc aaaaggtcaa gaaaattagt ctgaggctta cacagttgaa gcaaaagagg
     2101 ctgttgctgg cgggcgaaat ctgcctataa ccgtcattag ctgcccaact gtgtgatttc
     2161 tgtggttaat aaaatgaaga aaaaattcct tggttatcaa tgctccttat gtaatacaca
     2221 atacgcgccg gatgaggtca cctacacttg ccccaaagat ggtggtaacc tggatgtcat
     2281 cctggactat gaggcaataa agcgagagac caccccggaa aaaattgcgg ctggtgagga
     2341 aagctcgcta tggcgctacc tgccccttct accggtggct gaccctcagg gattggggac
     2401 accgctgagg gcggctggtg gtacgccgct cttcgctccc aagcagctgg cagaaaatct
     2461 gggattgcgc tccctgtggg tgaaggacga gagccgtaac ccaacagcct catttaagga
     2521 tagagcgagt gctgtggttg tagcgcgcgc tcgtgaggtc ggggctgaag tagtggtcac
     2581 cgcctcgaca ggcaatgccg gggcagccct ggcgggtatg gccgccgcag ttgggcagca
     2641 ggcggttatt ttcgctccaa agacagctcc ccctgccaaa attgcccagc ttctgattta
     2701 cggggcgagg gtattgttgg tggatggcac atacgatgac gcctttgacc tgactattca
     2761 agctgcacag gagtttggct ggtattgtcg aaacacggga tacaatccgt tcaccgcgga
     2821 gggcaagaag acggctgcgt tggaaatctg ggagtgggta aaaattcctc gagcaaccat
     2881 atttgtctct gtcggcgatg ggaacatcat ctctggcatc cacaaaggct ttaaggacct
     2941 gctcgagctg ggctggatgg agaacatgcc tcgcattttt ggagtgcagg ctgaaggttc
     3001 tgccgccgtt gcgaatgctt tccatgccag gacggagacg atcaccccgg tttctgcccg
     3061 cacacttgcc gatagcatct ctgtagacct gccgcgagat ggtgtgcgcg ccgtgcgcgc
     3121 cgctcgcgat
//
LOCUS       DBNHOH010000432         3623 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_344599, whole genome shotgun sequence.
ACCESSION   DBNHOH010000432 DBNHOH010000000
VERSION     DBNHOH010000432.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_344599"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..566)
                     /locus_tag="AC16V7_07620"
     CDS             complement(189..566)
                     /locus_tag="AC16V7_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257026.1"
                     /translation="MGPRQQFCPNMTYPARGKVGENIIVVHSRKEANYQCKYMVRPLL
                     QRPGRHSTECIIRWMVIVVTLIAHGCPKAAQGLLKISQGRTKLNTAFIERLNATFRSC
                     LAVLVRRSRALIRNPQSWSHSRI"
     gene            673..2367
                     /locus_tag="AC16V7_07625"
     CDS             673..2367
                     /locus_tag="AC16V7_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR6257027.1"
                     /translation="MKGKIQTSSIPNRKQSSGILLTVLAIWHVLLALGCLAGAVVLWQ
                     RYTPEATWLQWLYIIVIVLIAASSAITVPLILRREHRGRILSLAINYLGFLICLFGSM
                     HALGIFVGIDSLASSFGRGILFLAGVLSGYLITTIGDRYENHPGRERFFRRLGKIIMA
                     IFGILFLFAVGILPGIISLLSRFNSPVTLALGVGVLLFGLMIWAMWQQPSAQAMGAKN
                     IHEEILNGYLFLSPNLFGFLFFFAGPLLLSFYVSFTDWDAFGSKNWIGLTNYAKIVNL
                     DIATLEDPKQRASEVLDVKVYDELTRFSLFGKHIIIGAEDKLFWLALRNTFIFSVLAV
                     PLSIIPALFLANLLNSKLPGMKFFRAIYFLPSIAAVVGVALIWQWLYNAAVGYINYGI
                     TSMVGLINSVTDSALIDPQIRWLSDTRTALLAVVIMSAWQWIGFNTVLFLAGLQNIPG
                     ELYEAATVDGAGQWAKFWNITIPLLAPTTFFVVTTAIIQALQLFEQVYVLIPTEPPGG
                     PNNATLSLVLYLYQKGFQRFDQGYASAVAWVLFVVIFSVTLFQFLRQRSGRGAYSV"
     gene            2384..3598
                     /locus_tag="AC16V7_07630"
     CDS             2384..3598
                     /locus_tag="AC16V7_07630"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR6257028.1"
                     /translation="MRSYHLGRLITNTLVYIILILFAFFMLFPFIYMFSTSLKYQKDT
                     FRYPPRLMPMQGITTQVEGFDEPLPLFYVDYNGEERKFALTESGIKIGIYAEPNNLEV
                     TYERPLTQVKPTGGVVNQAIVMVNGKEEKLFDVDVEGQMIPMIKVGQTALGNFIDPSD
                     PSIFVLQNVRLSQPVEEPAGRPENYKEVTELQGLDRSLTNTALVTILVVIGQLVTSVL
                     GGYALARLRFPGRDQIFVIYLGTIMIPFVVLITPLYQLMVMIGWVDKLAALIIPWIFT
                     AYGTFLMRQFFISYPKEIEESALIDGASRLQILVSILLPASVPALAALATFTFLYAWN
                     SFFWPLVIINTGNRANHVLTLSLSILRGRASDNANLVMAGAAIDILPPMIIYILAQRF
                     FVESATSSGVKG"
BASE COUNT          808 a          948 c          901 g          966 t
ORIGIN      
        1 atgttttgag ggtcgctact catgcggtgg tatgagaaat agaagtagct ccttaacagt
       61 ccaaatatga tcggtaaggc ccgcagcgat ggctggggat cgttgtaccc agcggaagcc
      121 atggctgtcc acccactgct tcaagagcag gctcttgtgc tcggggcaga agatgtagac
      181 acagcccatc agatacgtga gtggctccaa gattgcgggt tgcggatcag cgctcgactg
      241 cgccgtacca ggactgccag gcaagagcgg aaggtggcgt tcagccgctc gatgaaagcg
      301 gtgttgagct tggttctgcc ttggcttatt ttcaacagcc cctgagcagc tttcggacag
      361 ccatgcgcta tcagggtcac tacgatgacc atccaacgaa tgatgcattc ggtagaatgg
      421 cgacccggtc gttgcagcaa aggccttacc atatatttgc attggtaatt tgcttctttt
      481 cggctgtgaa caacgataat gttctcacct actttgcccc ttgcagggta ggtcatattg
      541 gggcagaatt gttgtcgagg gcccatgtgg cttacgcttt tcaggtggta gaacctttag
      601 agtgccacag gaccttcatt ttttcaacct ccacgtttta ttggagtgct accacacgca
      661 caggaggatg ttatgaaagg aaaaatccaa acatcatcca tccccaatcg aaaacaaagc
      721 tcgggaatct tgctaactgt gctcgccatc tggcatgtac tcctggcatt aggctgccta
      781 gcaggtgcgg ttgtgttgtg gcagcgctac acacccgaag caacctggct acaatggctg
      841 tatattattg tcatagttct catcgcagcc tcaagcgcca tcacggtgcc gctgatctta
      901 cgccgcgagc accgagggcg catcctatcg ctcgccatta actatctggg tttccttatc
      961 tgcctgtttg ggtccatgca tgcgcttggc atattcgtgg gcatcgactc gctggcaagc
     1021 agcttcgggc gtggaattct attccttgct ggcgtattat ccggttatct gatcacgact
     1081 atcggcgatc gctacgaaaa ccatcccgga cgggagcgat tcttccgccg ccttgggaag
     1141 atcatcatgg cgatcttcgg catactcttc ttgtttgctg tagggatttt gcctgggatt
     1201 atctcgctgt tatcgcgctt taactccccg gtaacattag ctctgggtgt tggcgtgctg
     1261 ctatttgggc tgatgatctg ggcaatgtgg caacagccaa gcgcgcaggc aatgggcgcc
     1321 aagaacatcc atgaagaaat cctcaacggt tatctatttt tatcccctaa cttgttcggc
     1381 ttcctgtttt tctttgccgg acctttgctt ttatcgtttt atgtaagctt caccgactgg
     1441 gatgctttcg gctcaaaaaa ttggattggg ctgacgaact atgccaagat tgttaatctt
     1501 gatattgcca ccctggaaga tcctaaacaa cgcgcttcag aggttctgga tgtaaaggtc
     1561 tacgacgaac taacccgttt cagcttgttt ggaaaacata tcattatcgg ggcagaggat
     1621 aaactcttct ggctggcgct gcgaaacaca ttcatattca gcgtgctggc tgtcccgctg
     1681 agtattattc ccgcactatt cctggcgaat ttactgaaca gcaagctgcc aggcatgaaa
     1741 ttttttcgag ccatctattt ccttcccagc atcgcagctg tagttggtgt ggctttgatc
     1801 tggcagtggc tgtacaacgc ggccgtaggt tatattaact acggtatcac cagcatggtg
     1861 ggcttaataa attcagtgac ggattctgcg ctcatcgacc cccaaatccg ctggttatcg
     1921 gacacacgca ccgctctact tgctgttgta atcatgtcag cctggcaatg gatcggcttt
     1981 aacacggttt tattcctagc cgggttacaa aatatccccg gcgaactcta cgaagctgct
     2041 accgtagatg gagccgggca atgggcgaaa ttctggaaca taaccatccc tttgctggca
     2101 ccaaccacct tctttgtagt tactaccgca atcatccagg ctctgcagct tttcgagcaa
     2161 gtctatgtgc tgatccccac cgaaccaccc gggggtccca acaatgccac actgtctttg
     2221 gtactttacc tctatcaaaa aggcttccag cgttttgatc agggctatgc ctccgcggtt
     2281 gcctgggtat tgtttgtggt cattttcagt gtgacccttt tccaattcct gcgccagcgc
     2341 tccggaaggg gcgcgtatag tgtttgagtt ccggaggcca gtcatgcgct cttatcatct
     2401 gggcagattg ataacaaaca cactggtgta tatcatcctt atcctgtttg catttttcat
     2461 gctcttccca tttatttaca tgttctcaac ctccttgaaa taccagaagg acactttccg
     2521 ctatccgccg cgcttgatgc ctatgcaagg cataaccacc caggtggaag gttttgatga
     2581 gccgctgccg cttttctatg tcgattacaa tggcgaagaa cgcaaattcg ctttgacaga
     2641 gagcgggatc aagatcggta tttatgctga gccaaacaac ctggaggtga cctacgagcg
     2701 cccattgacc caggtcaagc caaccggcgg tgtagttaac caggccattg tgatggttaa
     2761 cggaaaagaa gagaaattat tcgatgtaga cgtcgaaggg cagatgatac ccatgatcaa
     2821 agtaggtcag acagctcttg ggaattttat agatccgagc gacccgtcta tatttgtgct
     2881 tcagaacgtc cgcctgagcc aacccgtcga agagccggct ggcagaccgg aaaattacaa
     2941 agaggtgacc gagcttcagg gactggaccg cagcctgacc aacacagcct tggtcaccat
     3001 cctggtagta atcggccagc tagtgacatc tgtgctcggc ggttacgcgc tggcgcgcct
     3061 tcgcttcccg ggccgcgacc agatatttgt catctatctg ggcacgatta tgattccatt
     3121 cgttgtgctg atcacccccc tttaccagct gatggtaatg atcggctggg tggataagct
     3181 ggctgctctg atcatcccat ggatttttac cgcatacggg acttttctga tgcgccagtt
     3241 cttcatatca tatccaaaag agattgaaga atcagcactg atcgatggcg cttcgcggct
     3301 gcaaatcctg gtgagcatcc tactgccggc aagtgtcccc gcgctggctg ctctcgccac
     3361 ctttaccttt ctatacgcct ggaatagctt cttctggcct ctggttatca tcaacacagg
     3421 caaccgcgcc aaccatgtgc tgactctttc actgagcatc cttcgtggcc gcgccagcga
     3481 taatgccaat ctggttatgg cgggcgcagc aattgacatc ctgccgccga tgatcattta
     3541 tatcctggct caacgcttct ttgtggaaag tgctaccagc agcggggtaa aagggtaaca
     3601 ataaccgccc attaccgttc cgg
//
LOCUS       DBNHOH010000433         3259 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_344728, whole genome shotgun sequence.
ACCESSION   DBNHOH010000433 DBNHOH010000000
VERSION     DBNHOH010000433.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_344728"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="AC16V7_07635"
     CDS             complement(<1..315)
                     /locus_tag="AC16V7_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257029.1"
                     /translation="MTVCRYCTPEWLEESAKIYRSSPKFKQALEKLSTRICFRVLAEP
                     VWGIDEDIIFGGTVDKGELVELVFFNEESARNKADFILSATPQEWKRLLRKESKFVTE
                     FML"
     gene            complement(356..2053)
                     /locus_tag="AC16V7_07640"
     CDS             complement(356..2053)
                     /locus_tag="AC16V7_07640"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR6257030.1"
                     /translation="MSQATYLDKPWIKSYRAGPYKLDASLAPYPREPVFKALDDAAQN
                     YPGQTAVLYLGRSLKYSQLKNQVDRLAAGLVRLGIEKGDRVCLFLPNCMEFIVSDWAI
                     MKAGGAIVPTSILRTDEGLLHEVSSSKSKIVICQEDKLERVLAIKEQSDIETIIVTSC
                     SGYDVESVATSLPRGCLDFRRLLHDNDLVTPAVNIDAEQDLCELAFTGGATGAPKGVM
                     ISHFNRYSCILQGFPWLLKPLLRGFVGKASTLISIPLFHSYGHYAYQASTYLGLRTIL
                     LPDPRDTDMMVDSIQTYRPFMIPGVPTQFMRIAEANLSRMNVLLLSGSAPLPLEVAEA
                     IKKKTGMPISEGYGLTETSPSTHYNLSAFSKIFGFMSKEKFGIGIPTPDTECKLVDPE
                     TGQMVPFGESGEMLIRGPQVMMGYWPQAGLGLTQDGWLHTGDIAVMDEDGYFQVVDRI
                     KDMVNISGLKVYTTQVDDVLYKHPAVLMAAAFGVPDPQIPGSERVMAVIQLKEGYQGK
                     VQAEDILELCKAHLAPYAVPKFVEFRDEMPLTVTEKVYKKALRDEILSKMKASGDNR"
     gene            complement(2074..>3259)
                     /locus_tag="AC16V7_07645"
     CDS             complement(2074..>3259)
                     /locus_tag="AC16V7_07645"
                     /inference="COORDINATES: protein motif:HMM:NF013481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HIR6257031.1"
                     /translation="VGFDAFGTNMVADMVNVRGGYPTHNWQEGVFEFIDAVNAQGLTD
                     TVFVRGVNCFACPVSCGRGTEIREGKWQGNKGEGPEYETTNTLGALCGVSDLNAITMA
                     NYRCNQFGLDTISAGATIAFAMECYRRGILTPEQTHGLEINFGDADLVVNLVEKIALR
                     EGVGDLLAEGSRGMAQRLGQGSEHFAMNVKGLELPAYDPRAAKLCGLAYVTANRGGDH
                     MTAYIEGPTFVDVPFLLVDDSHIQDPFTANPQEARIVVDLENALTVFDSIGGCKFMGI
                     LLTAEDMTNLLANATGWQIGVDDFRKSGERIYNLMRAFCVREGIDRHQDTLPGRLLED
                     PLPGGPAEGMVIERDSLEKMKDAYYEMRGWDLATGIPTPQKLSELGLEYLIADLWNAK
                     PN"
BASE COUNT          720 a          895 c          832 g          812 t
ORIGIN      
        1 caacataaac tcggtaacaa atttgctctc tttgcgcagc agccttttcc actcttgagg
       61 ggttgccgac agtatgaaat cagctttgtt cctggcactt tcttcattga aaaagaccag
      121 ctcaaccagt tctcctttat caacagtgcc accaaagata atatcctcat cgatccccca
      181 gaccggctcc gccaggactc gaaaacagat acgggtggat aatttctcca atgcctgctt
      241 gaattttggt gaagagcgat atatcttggc gctctcctca agccattcgg gggtgcaata
      301 tcgacagact gtcatctctc cgcctccatt gaggactagc gcttgagccg ccctcttatc
      361 gattgtctcc actagccttc attttggata gtatttcgtc tcgtaacgct ttcttatata
      421 ccttctccgt aaccgtcagc ggcatctcgt ctctgaattc gacgaactta ggtactgcgt
      481 atggcgcaag gtgagcttta cataattcga gtatatcctc cgcttgcact ttcccctggt
      541 agccctcctt taactgaatg acagccataa ctcgttcgct gccaggaatc tgggggtcgg
      601 gcacgccaaa ggcagccgcc attaagacag ctggatgttt gtacagcaca tcatcgacct
      661 gggtggtgta gaccttcagc ccggagatat tcaccatgtc tttgatccgg tcgacaacct
      721 ggaaataacc atcttcatcc atcacggcaa tatcgccggt atgaagccag ccatcttggg
      781 tcaaccccag gcctgcctgg ggccagtatc ccatcatcac ctgagggcct ctgataagca
      841 tctcgcccga ttctccgaat gggaccattt ggcccgtttc cggatcgacc agcttgcatt
      901 ctgtgtctgg ggtggggatt ccgatgccaa atttttcttt cgacataaaa ccgaaaatct
      961 tggagaaagc agacaggttg tagtgggtgc taggagatgt ctctgtcagc ccgtagccct
     1021 ccgagatagg cataccggtt ttcttcttga tcgcctcagc cacttccagc ggcagtggcg
     1081 cagagccact tagcaggagg acattcatgc ggctcagatt tgcctcggcg atgcgcataa
     1141 actgagtggg caccccaggg atcatgaagg gacggtaggt ttgaatactg tcgaccatca
     1201 tgtcagtgtc ccgtggatcg ggtaagagga tggtgcggag ccccaaatat gttgaagctt
     1261 ggtaggcgta atgcccgtag gaatgaaata agggaatgga gattaatgtc gaggcttttc
     1321 ccacaaaacc ccgcagcagg ggcttgagca gccagggaaa tccctggagt atgcaactat
     1381 agcggttgaa atgagagatc attacgcctt tgggggcgcc ggtcgcccca ccggtgaatg
     1441 ccagctcgca caaatcttgc tccgcgtcga tgttcaccgc aggcgttaca agatcgttgt
     1501 catgtaggag ccggcgaaaa tcaaggcaac ccctcgggag cgaggtggca actgattcga
     1561 catcatagcc gctgcaagaa gtgacgatga tggtttcgat gtcagactgc tctttgattg
     1621 ccagcacacg ctccagtttg tcttcctggc aaatgacgat cttgcttttt gagctggata
     1681 cctcgtgcaa taagccctcg tcagtgcgca ggatgctggt tggcacaatt gcaccgccgg
     1741 ctttcatgat agcccaatcg ctgacgataa actccatgca gttaggcagg aagaggcaaa
     1801 ctcggtctcc tttttcgatc cccagcctga ccagtccagc cgccagccgg tcgacctggt
     1861 tctttaactg gctgtacttt aacgacctcc cgagatatag aactgcggtt tggccggggt
     1921 aattttgggc tgcgtcatcc agcgccttga agacgggttc tctgggatag ggagctaggc
     1981 tggcatctag tttatagggc cctgcccggt agcttttgat ccagggttta tccagatagg
     2041 tggcttgact catggcaaaa aatggctttc ccgctaattt ggttttgcat tccagagatc
     2101 tgcgatcaga tattccagcc ccaattcact cagcttctgg ggagtaggga tgccggtagc
     2161 gagatcccag ccgcgcattt cgtaataagc gtccttcatt ttctctaggc tgtcgcgctc
     2221 gatgaccatc ccctctgctg gtccgcccgg caatgggtct tccagcaagc gacctggtag
     2281 tgtgtcttga tgccgatcga tcccctctcg cacacaaaaa gcgcgcatga gattgtagat
     2341 gcgttccccg ctcttccgga agtcatctac cccaatttgc catccagtgg cgttggcgag
     2401 taagttggtc atgtcttcag ccgtcagaag aatacccatg aacttgcagc cgccgatgga
     2461 gtcaaaaaca gtcagggcgt tttcaagatc aacgacaatt ctggcctctt gcgggttagc
     2521 agtgaacgga tcttgaatat ggctgtcatc aaccagcagg aagggcacat ccacgaaggt
     2581 ggggccttcg atgtaggcgg tcatgtggtc tccccctcgg ttggctgtta cataagccag
     2641 gccgcagagc ttggctgccc tgggatcata ggcaggtagc tcgagccctt tgacgttcat
     2701 ggcgaagtgt tcgctgcctt gacctaatct ttgtgccatg cccctgcttc cctccgccag
     2761 tagatcgccg acgccttccc ggagagcgat tttttctacc aggttcacca ccaggtcggc
     2821 gtctccaaag ttgatttcaa gaccatgcgt ctgttcggga gtgaggatac ctctgcggta
     2881 gcattccatg gcaaaggcga tggtggcgcc agcgctgatg gtatccagac cgaactggtt
     2941 gcaacgatag ttcgccatgg tgatggcatt taaatcggaa acgccgcaca aagcacccaa
     3001 tgtattggtg gtttcatatt ccggcccttc gcctttgttc ccctgccatt tgccttctct
     3061 gatttcagtc ccgcgaccac acgagaccgg gcaagcaaag caattgacgc cccggacgaa
     3121 tactgtgtcc gtcaagccct gcgcgttgac agcatcaatg aattcaaaga ccccttcctg
     3181 ccagttgtgc gtcgggtatc cgcctcgcac gttcaccata tcagcaacca tgttcgttcc
     3241 aaaggcatca aaaccgact
//
LOCUS       DBNHOH010000434         3781 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1242969, whole genome shotgun sequence.
ACCESSION   DBNHOH010000434 DBNHOH010000000
VERSION     DBNHOH010000434.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1242969"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="AC16V7_07650"
     CDS             <1..970
                     /locus_tag="AC16V7_07650"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR6257032.1"
                     /translation="PKPGAGQALVWLRAAALNRMDLWVREGWPGIKLEYPHISGADGA
                     GEIAALGDGVTGWSIGDQVVINPSLNCGHCEFCLAGQDNLCNKWALLGETVRGTLAEY
                     VVVPSTNLFSLPADYDPHQAAAAALVFQTAWHSLITRGGLRPGETVLVVGASGGVNTA
                     AIQIAKLAGAVVYVVGSSEDKLALAESLGADYLIDHTKEENWSKAIFQLTNRRGVDVV
                     VDNVGTTFPQSFRTARKGGRILTVGNTGGPKFEIDNRYIFGKHLSIIGSTMSTHQDFA
                     RVMELIFAGKLKPVVDRPFPLSETRAAEEHLEKGNQKGKVTIDIRG"
     gene            1191..1646
                     /locus_tag="AC16V7_07655"
     CDS             1191..1646
                     /locus_tag="AC16V7_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257033.1"
                     /translation="MSNKNLQPSNASFGAQFSSTYPRPFSVTLLVFGVLTIAGINLYR
                     CIEAIVQWRFLAGLLPALVSYIMLSGLFWSLIGIPLAWSLWRGNRRAPQMTLWIVLVY
                     ITYYWLDRLLIRQGSASDNLPFTLGFAVLVLAFVFWTLSRPVAKAFFDT"
     gene            1745..3298
                     /locus_tag="AC16V7_07660"
     CDS             1745..3298
                     /locus_tag="AC16V7_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR6257034.1"
                     /translation="MSEIDPKIQELRKLKSQSRLGGGAERIESQHQRGRLTARERIDL
                     LMDKGSFREIDAFAEHRTTDFGLEKQKYLGDSVVTGWGTIGGRLVYVFSQDFTVFGGS
                     LGEVHAEKICKIMDMAMKNGAPLIGLNDSGGARIQEGVVSLGGYADIFLRNTLASGVI
                     PQISVIMGPCAGGAVYSPALTDFIIMVRNSSYMFVTGPEVVKTVTHEEVSFEELGGAS
                     VHSETSGVCHMAADSEADALYLIQKMLSYLPQNNMEDPPFVPNGDDPLRMEEALDKLI
                     PDDPGKPYDIKNAIRLIVDDRQFFEIHEAYAQNIVVGFARLGGHSIGIIANQPAVLAG
                     VLDINASEKAARFVRFCDAFNLPILTFVDVPGFLPGTAQEHGGIIRAGSKLLYAYCEA
                     TVPKLTVVTRKAYGGAYDVMSSKHIRGDVNLAWPTAEIAVMGPDGAVNIIFRRELAQA
                     KDPAKRKAELVDEYRKKFANPYVAASRGYIDDVIEPRETRPRLINALEMLSNKRDTNP
                     PKKHGCIPL"
     gene            complement(3315..>3781)
                     /locus_tag="AC16V7_07665"
     CDS             complement(3315..>3781)
                     /locus_tag="AC16V7_07665"
                     /EC_number="2.8.3.-"
                     /EC_number="4.1.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-transferase subunit beta"
                     /protein_id="HIR6257035.1"
                     /translation="GAQVDRVGNLNTSYIGPVRQPQTRLPGSGGACDLASLAKRHIIV
                     MPHQKRRFVPKVDYITSPGFGDGRGWREAQGLPRGGPAAVITTLGVLHFDPSTFEMVL
                     TSIHPGVTVEEVQANTGWALRVAPDITQTPEPSDEQLAILRKNDLEGYWTGV"
BASE COUNT          875 a          992 c         1046 g          868 t
ORIGIN      
        1 ccccaagcca ggagcaggac aagcactggt atggctcagg gcggctgcat tgaaccgcat
       61 ggatctttgg gtgcgggaag gctggccagg catcaaactc gaataccctc atatcagcgg
      121 ggcggatggt gctggggaaa tcgccgccct gggggatggc gtcacaggat ggtcgattgg
      181 tgaccaggta gtgatcaatc ccagcctgaa ctgtggacat tgcgagttct gcctggctgg
      241 tcaagataac ctgtgcaaca aatgggctct actcggtgaa acagtgcgcg gcacgttggc
      301 ggaatatgta gtcgttccga gcactaacct cttctcgctt cccgctgact acgacccgca
      361 ccaggctgct gcagcagcgc tggtctttca aaccgcctgg cactcgctca tcacgcgcgg
      421 cgggctgcgc ccaggtgaga cagttctggt ggtaggagct tcgggagggg tgaacaccgc
      481 agctattcag attgccaaat tggcgggagc cgttgtctac gtggttggtt caagcgagga
      541 caaactggct cttgcagagt ctctaggggc agattatctg attgaccaca ccaaggagga
      601 gaactggtcc aaggccatat tccaattgac caaccggcgc ggtgtggatg tcgtggtgga
      661 caatgtgggc accaccttcc cgcagagctt tcggacagcg cgcaagggtg ggcgcatcct
      721 gactgttggc aatactggcg gcccaaagtt tgagatcgat aaccgttata tatttggcaa
      781 acacttgtcc attattgggt cgacgatgag cacccaccag gactttgccc gcgtgatgga
      841 actgatcttt gctggcaagc tcaaaccagt tgtggatcgt ccgttccctc tctcagagac
      901 gcgtgcagcg gaggaacacc tagaaaaggg gaatcaaaag gggaaagtca ccattgatat
      961 tcgaggataa tggctggaga caaacccaaa ccttcagggc aattgcatat gcccggaatg
     1021 gcatgcgcaa tcccgcaaaa agagtgtttg gggtgttgtg gtgggggtat gccccaccac
     1081 aacaccccat tttctctcct cagtggtggg gaatcgccca aatgcgattg ccctacccaa
     1141 accttggatt gatcacaaaa gggtgatcag ttaagggtag aattctttct atgtccaata
     1201 agaatctgca gccctcgaac gcttcatttg gcgcccaatt ttcatcaacc tatcctcgac
     1261 ctttcagcgt aaccctgctg gtatttgggg ttttaaccat tgctgggatt aacttatatc
     1321 gctgcattga agccatcgta caatggcgct ttctcgccgg gctgctcccg gcgcttgtat
     1381 cttatattat gctaagtgga ttattttgga gcctgattgg gatcccttta gcctggagcc
     1441 tatggcgcgg caatcgcagg gcacctcaaa tgaccttatg gattgtcctg gtgtacataa
     1501 cctactactg gttagaccgg ttgcttatac gtcaaggatc agcatccgat aacctgccct
     1561 tcacactggg attcgctgtg ctggtgctag catttgtttt ttggacgtta tctcgaccag
     1621 ttgccaaagc atttttcgat acatagcaaa aaatagccta taatcagctt ttctatattc
     1681 ccgtatacag catgggtaaa attgaaatgc gatcaagaag gaagcttatc ttttggagcg
     1741 ttgaatgagc gaaattgatc ctaagatcca agaattacgc aaactgaaaa gccagagccg
     1801 gctgggcggc ggtgctgagc gcattgaatc gcaacaccag cgcggccgcc tgaccgcacg
     1861 cgagcgcatt gatctgctga tggataaggg ttctttccgc gagattgatg catttgcaga
     1921 acaccgcacg acagattttg gcctggaaaa acagaagtac ctgggcgaca gtgtagttac
     1981 cggttggggc accattggcg gtaggttggt ctatgtcttc tcgcaagatt ttacagtctt
     2041 tggcggcagc ctgggagagg tgcatgctga gaagatctgc aagataatgg atatggcaat
     2101 gaagaatggc gcgcccctaa tcgggttaaa cgattccggc ggggcacgca tccaagaagg
     2161 ggtagtctcc ttgggaggtt atgcagatat attcctgagg aacaccctgg cttctggcgt
     2221 tatcccccag attagtgtga ttatgggacc ttgtgccggg ggagcagtct actctcctgc
     2281 cctgactgac ttcatcatca tggtgcgcaa ctcctcgtat atgttcgtca ccggaccaga
     2341 ggtggttaag acggtgacac acgaggaggt ttccttcgaa gagttgggcg gcgccagtgt
     2401 gcactctgag acgtctggcg tctgccacat ggctgccgac agtgaagctg atgccctcta
     2461 tcttatccaa aagatgctct cctacctgcc ccagaacaac atggaagacc ctcccttcgt
     2521 gcctaacggg gatgatccac tgcgcatgga agaggcgctg gataaactga tccctgacga
     2581 cccggggaaa ccctacgaca taaaaaatgc gatccgcctg atcgtggacg acaggcaatt
     2641 ttttgaaatc cacgaagcat acgcgcaaaa catcgtcgtg ggcttcgccc gcttgggagg
     2701 ccacagcatt ggtattattg ccaatcagcc cgctgtatta gctggcgttt tggacatcaa
     2761 tgcatccgag aaagcagccc gctttgtgcg gttctgcgat gcattcaatc tccctatact
     2821 gacctttgtg gatgtgcctg gttttctgcc tggcactgcg caggaacacg gcggcatcat
     2881 tcgcgcgggc tccaagctgc tgtatgccta ttgcgaagcc accgtgccaa agttgacggt
     2941 ggtcacccgt aaagcttacg gcggcgcgta tgacgtgatg agcagcaagc acatccgagg
     3001 agacgttaat ctggcctggc ctacggcaga gatcgccgtg atggggccag acggcgcggt
     3061 aaacattatt ttccgcagag aactggcaca agcgaaagac cccgccaaac gcaaggctga
     3121 gttagttgac gaataccgaa agaaattcgc caatccgtat gtagcggctt cacgcggcta
     3181 tattgacgat gtgatcgagc cgcgcgagac gcgcccgcgc ctgatcaatg ccctggagat
     3241 gctctcaaac aagcgcgata ccaatccgcc gaagaaacac ggctgcattc cgctctagaa
     3301 tctctagctg ttggtcagac ccctgtccaa taaccttcca aatcattttt gcgcaagatt
     3361 gcaagctgct cgtcactagg ctctggcgtt tgtgtaatgt ccggcgctac acggagtgcc
     3421 caccccgtgt ttgcttgcac ttcctcaact gtaacccccg gatggattga ggtgagcacc
     3481 atctcaaagg tagacgggtc gaaatgcagc acgcccagag ttgtgatcac tgctgctgga
     3541 ccgccgcgcg gcaagccttg tgcttctcgc cagccccggc cgtctccaaa gccgggcgag
     3601 gtgatgtagt ccaccttagg gacgaaacgg cgcttttggt gaggcatcac aataatatgc
     3661 cgcttagcca gtgaagccag gtcgcaagcc ccaccgctac ccggcaagcg ggtctgtggt
     3721 tggcgcactg gtccaatgta gctggtattc aaattgccaa cacgatccac ctgagcgccg
     3781 c
//
LOCUS       DBNHOH010000435         3312 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1755499, whole genome shotgun sequence.
ACCESSION   DBNHOH010000435 DBNHOH010000000
VERSION     DBNHOH010000435.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1755499"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1479
                     /locus_tag="AC16V7_07670"
     CDS             <1..1479
                     /locus_tag="AC16V7_07670"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR6257036.1"
                     /translation="LVEKELITSSELQRALEYQKLKAASGQHRLLGRSLLDLGYIDRE
                     TLDKVIVSQIFSLHIALQQANQQLEQRVQQRTQDLEKRFDQIYTTSELAQIAVFATDL
                     DELLKRAVALIVERLGFDHAIIYLLDEAGDFAEPRAAFDAFGKDKDLGNYKLPINSHS
                     AVGWVASQNQTLVISDIHQESLFAQCEIFPTARSEVGIPIVPDEKLKGILNALHSNPD
                     AFDTDTVTTLQTIANLIASHIQNLSLLRATQSNLKVMEKRVAILETLDLVSKAISSDL
                     DLNTLFQLAHQQINQLMGDVDVTITFEDTNTNTAEVSYPAEPDGSSPKQSDISDQGLI
                     TRVLHTKSPLMLVEDAELQAAELGIPFTGAAAKSWLGAPLLVGGMAIGAIVVRDFHQK
                     HRFDEDDQRFLINLATQIAITVQNNQQLENARAQAERERIANEISSQLWSATDVQSIL
                     RTAIKELSNKLGAVEGVIHIHAPNAPNGITAQANNGDNKGSP"
     gene            1551..>3312
                     /locus_tag="AC16V7_07675"
     CDS             1551..>3312
                     /locus_tag="AC16V7_07675"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR6257037.1"
                     /translation="MYVLVLNAWDRANRHASVILCLVGINTFVVGWMIGVENLQQAHF
                     PALLLAITSPSIQPLLFVAIIAIVKPEWLISRNRWTGWMFYTLTIIPIAFTLYDQFFA
                     NPLWYSGLPFNYTGGFIPLGEVTQGLIADRIRFVSFTLLPALALLLLLYFAVFDKRVL
                     GGNRRLAWLLLIAQAASYMTAKVLSPQLLPAVAAMASSTLFITVFVYAFFLQILSERR
                     IQRGSLQFRLTASIMVVAIPLLIAITVFVNWRASSLINWGATTQLSESELLAGEMLFQ
                     RTSLISLAIGVMLLVLISWLTIRYALQPLIDLSNTANGVASGDLTRVAVVQTEDELGD
                     LARGYNSMITQVRETITSLQRRVAERDRDLERRTIQLQLSAEVARQSSANSSLAQLLD
                     RIVRLISDRFNIYHAGIFLIDEEGDHLVLRAASSPGGKRLVERNFKQAIDQTSLVGSV
                     ADKGELLLSQDIDKDTLAIGHADLPQTRSEIALPLKTSDLTLGVLDLQASKIAAFSND
                     DIEIFEILSDQIALAIDRAKLQTKNEELSREMGLLYQQQIHQSWQRRLEDLSMTFAYR
                     SGNVTREFSHDAIEEYEEQKT"
BASE COUNT          895 a          807 c          736 g          874 t
ORIGIN      
        1 ttagttgaaa aagagttaat tacctccagc gaactgcagc gggctctgga gtatcaaaag
       61 ctgaaagctg ccagcggcca acaccgctta cttggtcgct ctctattgga tttgggatat
      121 atagaccgag agaccctgga taaagttatc gtttcccaaa tattttcgct tcacattgct
      181 ttgcagcaag caaaccagca gcttgaacaa cgtgtccaac agcgcacaca agatctagaa
      241 aaacgttttg atcaaattta tactacctct gagctggcgc aaatcgcagt cttcgcaacg
      301 gatttggatg agttactcaa gcgagccgtt gcgctgattg tagagcgctt gggctttgat
      361 cacgccataa tttatctttt ggatgaagct ggtgactttg ccgaaccacg cgctgcattt
      421 gatgcttttg gcaaagataa agacttgggt aattacaaat taccgatcaa ctcgcactcc
      481 gctgtcggtt gggttgcaag ccaaaatcaa acccttgtga tctctgacat ccatcaagaa
      541 tccctattcg cacaatgtga aatattccct actgctcgtt ccgaggtagg aattcctatt
      601 gtgccggacg agaaactgaa gggtatcctc aacgcccttc attcaaaccc tgatgcattt
      661 gatactgaca cagtcaccac cttacaaacc atcgctaatt tgatcgcgtc acacattcag
      721 aatctgtctc tgctgagagc aactcaaagt aatctgaaag taatggagaa aagggttgcg
      781 atcctagaga cgcttgattt ggttagtaaa gctatctcat ccgatctcga tcttaacact
      841 ttatttcagc tggcccacca acagataaac caattgatgg gcgatgtaga tgtcaccatt
      901 acgtttgaag atacaaacac caataccgca gaggtgtcat atccagctga acctgatgga
      961 tcctcaccaa agcaatctga catctcagac caagggctaa ttaccagggt attgcatact
     1021 aaatcgccgc taatgctggt cgaagacgct gagctccagg cagcggagct cggaatccca
     1081 tttacgggcg cggcagccaa gtcctggctg ggagcacctc tactggtagg aggaatggct
     1141 atcggagcca tagtagtaag agactttcat caaaagcatc gcttcgatga agatgaccag
     1201 cgctttctaa tcaatttggc aacccaaatt gctattacag tccagaataa tcagcaatta
     1261 gagaatgctc gcgcccaagc agagcgcgaa cgtattgcta acgaaatctc gtcacaacta
     1321 tggtccgcaa cagatgtgca gtccattctg cgaaccgcta tcaaggaatt aagcaacaaa
     1381 ttaggagccg ttgaaggggt aatccatatt catgcgccaa atgccccaaa tggaataact
     1441 gcacaggcaa acaacggaga taacaaggga tcaccgtgac tctatcgatg atcgatcaat
     1501 caatccaatc tttagcctgg ttaattgcca tcattgagct cattctcggc ttgtatgtac
     1561 tggtgttaaa cgcttgggat cgcgctaacc gtcatgccag tgtgatactg tgcttggttg
     1621 gaatcaatac atttgtagtc ggctggatga tcggggttga aaacctgcaa caggcgcact
     1681 ttcctgctct cttgttagcg attacctctc catccatcca accattgctt ttcgtagcca
     1741 taatcgctat tgtaaaaccc gagtggctca tcagtcgaaa tcgctggaca gggtggatgt
     1801 tctacacgtt aaccatcatt ccaatagcat tcactcttta cgatcaattt ttcgctaatc
     1861 ccctctggta tagtggtttg ccattcaatt acacaggtgg atttatccct cttggcgagg
     1921 tgacccaggg tctgattgcc gatcgtatcc gatttgtctc ttttacactg ttgccagcac
     1981 tggcgttatt gttattgctc tactttgctg ttttcgacaa gcgtgtactc ggaggaaatc
     2041 gacggcttgc ctggttgcta ctcattgccc aagccgcatc ctatatgaca gcgaaagtat
     2101 tgtcaccgca acttttacca gcagtggctg caatggcttc cagcacacta tttatcaccg
     2161 tctttgtata tgcatttttc ctacagatct tatcggaacg tcgtattcag cgaggcagcc
     2221 tgcagtttcg gctaacagct tcgatcatgg ttgtcgccat cccacttctg attgcgatta
     2281 cggtattcgt gaattggcgt gcaagtagct tgataaattg gggtgccacc acccagcttt
     2341 ctgaaagtga attattggcg ggagaaatgt tatttcaacg cacgtcatta atctcccttg
     2401 ctattggcgt aatgcttctt gtcttgattt catggctgac aattcgttat gctctccagc
     2461 cattaataga tttatcaaat actgctaatg gcgtcgcatc gggggatctc acccgagtgg
     2521 ccgttgtcca aacggaagac gaattgggag atcttgcgcg tggttataac agcatgatca
     2581 cacaagttcg cgagacgatt acaagcctcc aacggcgtgt tgctgaacgc gatagagatc
     2641 ttgaacgtcg cacaatccag ttgcaattaa gcgctgaagt cgccagacag tcatcagcaa
     2701 atagcagtct ggctcagctc ttggatcgca ttgtccgctt gatatccgat cgcttcaaca
     2761 tataccatgc tggtatcttc ctgattgatg aagagggtga tcacctcgtc ttgcgcgcag
     2821 catcctcgcc tggaggaaag cgattggtgg agcgcaattt caaacaggca attgatcaaa
     2881 catctttggt agggtctgtg gcggacaagg gtgaactgct cctatcccaa gacattgaca
     2941 aggacaccct cgcaatcggg catgctgatc tcccgcaaac acgttctgaa atcgcactgc
     3001 cactaaaaac cagtgacctc acccttggag ttttagatct gcaagccagc aagattgcag
     3061 ctttttccaa cgatgacatc gaaatatttg aaatcctttc tgaccagata gcactcgcaa
     3121 ttgatcgcgc aaagctacaa acaaagaatg aagaactttc gcgtgagatg ggattgcttt
     3181 atcaacagca gatacaccaa tcatggcaga gacggctaga agacctgtct atgaccttcg
     3241 cttaccgctc tggcaatgtg acaagggaat tttcacatga tgccatagaa gaatacgagg
     3301 aacaaaaaac tc
//
LOCUS       DBNHOH010000436         4083 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_409181, whole genome shotgun sequence.
ACCESSION   DBNHOH010000436 DBNHOH010000000
VERSION     DBNHOH010000436.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_409181"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="AC16V7_07680"
     CDS             complement(<1..915)
                     /locus_tag="AC16V7_07680"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HIR6257038.1"
                     /translation="MPIWNPEYEAMPRDELKKLQGKRLRQIVEYAYNKMPFYQRKLKA
                     AGVNPEDINSLDDLKRLPYTTKADLREEYPFGTFAVPLDEVVRIHASSGTTGKPTVGG
                     YTRKDLEIWAEVMARTVTSCGVTSKDIAHNAYGYGLFTGGLGFHLGFETVGATTIPMS
                     GGLTRRQIMLMEDFGATVLTCTPSYSLVLGEEAAEIGVNFKERMKVRIGIFGAEPWTE
                     EMRGEIEEHLGLSAYDIYGLTEVIGPGVSVECEHRTGLHIQEDHFLPEIIDPDSGESL
                     DYEEEGELVFTALTKEAMPLIRYRTRDRT"
     gene            complement(956..1564)
                     /locus_tag="AC16V7_07685"
     CDS             complement(956..1564)
                     /locus_tag="AC16V7_07685"
                     /inference="COORDINATES: protein motif:HMM:NF013707.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HIR6257039.1"
                     /translation="MDNGTINFLLAGVGGQGTLLASDILAEVGLHVGYDAKKSEVHGM
                     AQRGGAVVSHVRWGKRVASPLCERGTVDYFVAQEMLESLRWLEYLRPGGTVVLSRQQL
                     PPTSTVFGSDVYPNDDQVIAEVSKLAGKVLALDAIGVAEGLGNRRIANSVLLGALSSE
                     LDLDPEAWITVIESKVPQRHKEANRKAFLAGREGQWELLRNP"
     gene            complement(1601..3379)
                     /gene="iorA"
                     /locus_tag="AC16V7_07690"
     CDS             complement(1601..3379)
                     /gene="iorA"
                     /locus_tag="AC16V7_07690"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HIR6257040.1"
                     /translation="MKSLMSGNEAIARGIWESGTEVATAYPGTPSTEILQNVRLYDDI
                     YAEWSANEKVALDVAIGSAYAGRRSVAAMKHVGLNVASESLFYASYTGINAGLVIITA
                     DDPGLHSSQNEQDNRHYARFAKVPMLEPTDSQEAKDYILRAFELSEQFDTPVLVRTTT
                     RTSHAKSVVALKDRQQVEKRPYVKNAPKYVMIPGYARLRHPVVEERMVKLAQWAEQTD
                     LNTIEWGDRSLGIITSGIAYQYAREVFPQASILKLGMVWPLPSEKIREFASQVSRLIV
                     VEELDPFIEENVRQLGLQVEGKSIFPLIDELTPERVRDAAIGANLLSGNGNGYKPSVG
                     PLPRRPPVLCAGCGHRDVFWVLRKVKAAVNSDIGCYALGVLPPLDSTDTIGCMGASIG
                     VAMGMRRGGLEKDNVAVIGDSTFFHAGLPALATAVYNNTPMVLIVVDNSTTGMTGHQG
                     NPASGETLRGENISRIDIAAVSRSMGVKHVTEVDARDLDAMETAVRVAIKSGETAVVV
                     AHTVCVFVTSHNRESYTVVTEDCNGCTLCYRIGCPAIYKSDILDVKTSRPKAWIDPKQ
                     CVGCGLCYDVCARQAIEPGAEKVVMV"
     gene            complement(3434..>4083)
                     /locus_tag="AC16V7_07695"
     CDS             complement(3434..>4083)
                     /locus_tag="AC16V7_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6257041.1"
                     /translation="LIGANGAGKSTLLNSVSGIVPSKRGVILLDGQEITHLPAETIVS
                     LGVSQVPERRQVFSSLTVPDNLLLGAYLRLRRGEKKAVYEDREYVYTLFPILRERQRQ
                     LAGTLSGGEQQMLAIGRGLMARPKVLLLDEPSLGLAPLLVKEIFQVLLRLRSEGTTIL
                     LVEQNARAALDVSNRAYVLETGRIVLQGTPKELISNQEVQKAYLGKSMNHRTPKP"
BASE COUNT          854 a         1169 c         1071 g          989 t
ORIGIN      
        1 cgtgcggtcg cgggtgcgat agcggatcaa tggcatcgcc tctttggtca gcgcagtgaa
       61 taccaactcg ccttcttcct catagtcgag gctctcaccg ctatcggggt cgatgatctc
      121 aggtaagaag tgatcttcct ggatatgcaa cccggtgcgg tgctcgcatt ccacggatac
      181 ccccggtccg atcacttcgg ttaatccgta gatgtcatag gcgcttaatc ccaggtgctc
      241 ctcgatctcg ccgcgcatct cttcggtcca gggctccgcg ccgaagatgc caatgcgcac
      301 cttcatgcgt tccttgaagt tcaccccaat ttcggccgct tcttccccca atacgagcga
      361 gtaggagggc gtgcaggtga gcacggtggc accgaagtcc tccatcaaca taatttgccg
      421 ccgggtgagc cctcccgaca tggggatggt tgtggcgcca actgtttcga accccaggtg
      481 gaagcccaac ccaccagtga atagcccata cccataggca ttgtgggcta tgtccttgga
      541 tgttactcca caggatgtga ccgtacgcgc catcacttct gcccaaattt ccaagtcttt
      601 gcgcgtgtag ccgcccacgg tgggtttgcc cgttgtacca ctcgaggcgt ggatgcgcac
      661 cacttcatcc aggggcactg caaatgtccc aaagggatat tcctcgcgca ggtctgcctt
      721 tgtcgtgtac ggaaggcgct tcagatcgtc gaggctgttg atatcctcag gattcacgcc
      781 agccgccttg agtttgcgtt ggtaaaaagg cattttattg taggcatatt ccacgatctg
      841 ccgcaatcgc tttccctgga gtttcttcag ctcatcgcgc ggcatcgctt catattcggg
      901 attccagata ggcatcgatt cctcctaaga taagaaatgt tttttataca cgcttttatg
      961 ggttgcgcag tagctcccat tggccttctc ggccggctaa aaatgctttg cgattcgcct
     1021 ccttgtgtct ttgggggact ttggattcga tcacagtaat ccatgcttct ggatcaagat
     1081 ccagctcgga tgaaagcgcg cccagcaaaa cgctgttggc gatgcggcgg tttcccagcc
     1141 cctcggcgac accgatcgca tccagagcga gaaccttgcc agccaatttg ctcacttctg
     1201 caatcacctg gtcatcattt ggatacacat cgctgccgaa tacggtcgag gtgggaggca
     1261 attgctgcct gctgaggaca accgtgcctc ctggcctgag atattccagc caacgcagag
     1321 attccagcat ctcttgagcc acaaaataat ccaccgttcc acgctcgcag agaggcgaag
     1381 ccacgcgttt tccccagcgc acatgactta ctacagctcc gccgcgttgc gccataccgt
     1441 ggacctccga ttttttggca tcatagccaa cgtggagacc tacttcagcc aggatatcgc
     1501 tggccaacag cgttccctgc ccacccacac ctgcaagcaa aaagttaatt gttccgttat
     1561 ccataacatc tctctcctat ctagccgggg taggctgatc tcaaaccata acaacttttt
     1621 cagcgccggg ttcaatcgcc tggcgggcgc atacatcgta gcacagcccg caacccacac
     1681 actgctttgg atcaatccag gctttggggc ggctggtttt tacgtccagg atgtcgcttt
     1741 tgtaaattgc cggacaacca atgcggtagc acagcgtgca accgttgcaa tcttcggtga
     1801 ccactgtgta ggattcgcgg ttgtggctgg ttacaaacac gcacacggta tgagcgacca
     1861 ccactgcggt ttcgcctgat ttgatggcga cgcgcacggc ggtttccatt gcgtccaaat
     1921 cgcgggcgtc gacttcagtg acgtgcttca cgcccatcga tcgagacacc gcggcgatat
     1981 cgatgcggga gatattctca ccccgcagtg tctctcctga agcagggttg ccctggtgac
     2041 cggtcatccc ggtggtgctg ttatccacca caataagcac catgggagtg ttgttgtata
     2101 ccgctgtcgc aagggcgggg agacctgcgt ggaagaaagt gctgtcgcca atcacggcta
     2161 cattgtcttt ttcaagaccg ccacggcgca tacccatcgc caccccgata ctggctccca
     2221 tgcagccgat cgtatcggtg ctatccagag gtggcagaac acccagggca tagcagccga
     2281 tgtcggaatt tacggctgct tttactttgc gtaataccca gaacacatct cggtgaccac
     2341 agccagcgca cagtacgggg gggcggcgag gcagcggtcc caccgatggt ttgtagccgt
     2401 tgccattccc gctcaacaga ttcgcaccaa tggcggcatc gcgcacccgc tcaggtgtta
     2461 gttcatcgat taatgggaag atgctttttc cttccacctg aagcccaagt tgcctgacat
     2521 tttcttcaat gaaagggtct aactcctcca ccacgataag gcgggaaacc tggctggcga
     2581 actctcggat tttctcggat gggaggggcc agaccatacc aagctttagg atcgaggctt
     2641 gcggaaaaac ttctcgggca tactggtaag caattccgga agtgataatg cccaaagagc
     2701 gatcgcccca ttcaatcgtg ttgaggtcgg tctgttctgc ccattgagcc agttttacca
     2761 tgcgctcttc taccacggga tgtcgtagac gtgcatagcc agggatcatc acgtactttg
     2821 gcgcgttctt gacataaggc cgtttttcaa cctgctggcg gtccttcagc gcaaccaccg
     2881 atttggcatg tgaggtgcgt gtggtggtac gcaccagcac gggggtgtca aattgctcac
     2941 tgagctcaaa cgcccgaagg atataatctt tcgcctcctg gctgtcggta ggctccagca
     3001 tgggtacctt ggcgaagcgt gcgtaatggc gattgtcctg ctcgttttgc gatgaatgca
     3061 gcccggggtc gtcagcggta atgatcacca atcctgcatt gattccagtg tatgaggcgt
     3121 agaagagtga ctcagaagcc acgttcagcc cgacgtgttt catcgcggcg acactccgcc
     3181 gcccggcata cgcagagcca atcgctacat ccagggcaac tttctcgttt gcagaccact
     3241 cagcgtaaat gtcatcatat aatcttacat tttgcagtat ctctgtgctg ggtgttccgg
     3301 gataggcggt ggctacctcg gtgcccgatt cccagatgcc tcgggcaatt gcctcgttac
     3361 cgctcattaa ggatttcatt attcacctcc tcgtaaggtt gataccaata ctttatatat
     3421 atatatggac gggtcatggt tttggggtcc ggtgattcat gcttttgccc aaatatgctt
     3481 tttgcacctc ttgattagag atcaattctt tgggggtgcc ctgtaagacg atgcgtcctg
     3541 tctccaggac atatgccctg ttggagacat ctagtgcagc ccgggcattc tgctcgacca
     3601 ataagatcgt tgtcccttca gagcgcaagc ggagcagtac ctggaatatc tccttgacca
     3661 gcagaggagc cagacccagg gagggctcat ccaggagtaa gaccttaggc cgcgccatca
     3721 acccgcgacc gatggcaagc atttgctgct cgccacccga gagggttccg gctaattggc
     3781 gttggcgctc tcgcaggatg gggaacaagg tatacacgta ctccctgtct tcgtaaactg
     3841 cttttttctc gccacgccgc aagcgcagat acgcgcccaa caacaagtta tctggtacag
     3901 tgagcgagct aaacacctgg cgtctttcgg gcacttggct gacgcccagg cttacgatgg
     3961 tctcggcggg caggtgggtg atttcttgtc catccagcag gatgacgcct ctctttgagg
     4021 ggacgatgcc agagacacta ttcagtagcg tggattttcc agccccattt gcaccgatca
     4081 agg
//
LOCUS       DBNHOH010000437         1145 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1499084, whole genome shotgun sequence.
ACCESSION   DBNHOH010000437 DBNHOH010000000
VERSION     DBNHOH010000437.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1499084"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="AC16V7_07700"
     CDS             <1..381
                     /locus_tag="AC16V7_07700"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6257042.1"
                     /translation="ALSDRIGRKGLILAGWTIYGLVYLCLAFAKTGFQVWLFFSLYGI
                     YYALTEGTARAYIADLVSQDQRGGAYGLFHAAIGLASLPASLIAGILWQGIGPWAGFG
                     PSAPFIFGAGMAILSVGLLVSWVK"
     gene            complement(475..650)
                     /gene="ssrS"
                     /locus_tag="AC16V7_07705"
     ncRNA           complement(475..650)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="AC16V7_07705"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(704..>1145)
                     /locus_tag="AC16V7_07710"
     CDS             complement(704..>1145)
                     /locus_tag="AC16V7_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HIR6257043.1"
                     /translation="PSHHLFTSPMLEDIPYLDSEPGKARGQQYDLVLNNYEVGGGSIR
                     IHDRGLQEKVFQLIGLDAEVAQQRFGHMLEAFEFGTPPHGGIAPGIDRICMILADERN
                     IREVIAFPKNQAARDVMGNAPSPVESEQLKELHIKLDLKPERPN"
BASE COUNT          253 a          290 c          299 g          303 t
ORIGIN      
        1 gccttgtctg atcgtattgg tcggaaaggg ttgatcctgg ctggatggac catctatggt
       61 ttggtatacc tttgcctggc atttgccaag actggcttcc aggtgtggct gtttttcagc
      121 ttgtacggca tctattatgc actgaccgaa ggcaccgcca gagcttacat cgccgacctt
      181 gtctcacaag accagcgcgg gggagcatac gggcttttcc acgccgccat tggactggca
      241 tcactgccag catctcttat cgctgggatc ctatggcaag gcataggacc ctgggcaggg
      301 tttggtccaa gtgcaccttt tatatttggc gcaggcatgg ctatcttgtc agttgggctt
      361 ttagtgagct gggttaagta aatctgctct tgcatcgaaa aaaaacacct ctcgaggtca
      421 attggcctcg agaggtgtcg tctttctgga tatcacctag cggcgattca gaaatgacac
      481 cccactgtgc cgtgtgcagc taagttttga acctgccttc gcaggttggg acccgccccg
      541 agtgctccgc cttggctcag gcctatcgcg tcacactcgc aagattggga cccgatttgt
      601 gggtgttggc tcaaaaccgg gttgcagcaa cacgcacaca gcagggttat ttatcttata
      661 ccataaaacc atttgcgtga ataggtaaaa cgagattaat aatttagtta ggtcgctcgg
      721 gcttcaagtc cagcttgata tgaagttcct taagctgttc cgattctacg ggggagggag
      781 cattacccat aacatcacgt gctgcctgat ttttagggaa tgcaatcact tcgcgtatat
      841 tgcgctcgtc agccaggatc atacagatgc ggtcaatacc tggagcgatc ccaccatgag
      901 gcggcgtgcc aaattcaaac gcctcaagca tgtgcccaaa acgctgctgg gctacctctg
      961 catctagtcc gattagctgg aaaacttttt cctgtaatcc gcggtcatgg atacggatcg
     1021 atccgccacc cacttcatag ttattcaaca ccaggtcata ctgctggccg cgcgctttgc
     1081 caggttcgct atccaagtag gggatatctt ctagcattgg tgatgtgaaa aggtgatggc
     1141 tggga
//
LOCUS       DBNHOH010000438         1138 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_474088, whole genome shotgun sequence.
ACCESSION   DBNHOH010000438 DBNHOH010000000
VERSION     DBNHOH010000438.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_474088"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1138
                     /locus_tag="AC16V7_07715"
     CDS             <1..>1138
                     /locus_tag="AC16V7_07715"
                     /inference="COORDINATES: protein motif:HMM:NF036968.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR6257044.1"
                     /translation="PGLYASPLSALDAIPDEDAATSEIVQLGRTIGCFQIESPGMRAT
                     LREIQASSVDDLMVALALYRPGPLTGGLKDDFVRRHRGEAEVTHLHPALAPLLADTFG
                     VILYQEQVLRIAHELAGLSLADADLLRRAMSHFDPGKQMQTLQEKFIAGAAERKGVPA
                     DVAERIWELMAAFAGYGFPKAHAASYAQVAWQSAWCKAHHPAIFMAAVLANWGGYYGQ
                     RVYLTEARRMGLTLRPPHVNFARREFSVAYEKSQPLLYMGLDQVRELTRQTIERIIRL
                     RPFASLEDFLTRVNPRPGEIDNLIRAGALDVFGKIPALLRRLKGEIVRGGQLSLFVSD
                     EDADDWSLEEKVAAQEAILGVSVIAHPLEMVTDQIAEAGALSTL"
BASE COUNT          230 a          306 c          355 g          247 t
ORIGIN      
        1 atcccggact ttatgcttct cccttaagcg cgctggatgc tattccagat gaagatgcag
       61 ctacgtctga gatcgtgcag ctaggtcgta ccatcgggtg cttccagatc gaaagcccag
      121 gcatgcgcgc aacgctgagg gagatacagg ccagtagcgt ggatgatctt atggttgccc
      181 tggctttgta ccgacccggc ccgctcactg gaggactgaa ggacgatttt gtgcgccgac
      241 accggggtga ggcggaggtg acccacctgc acccggcgct cgccccgctg ctagcggata
      301 catttggcgt gatcctttac caggaacagg tgctgcgcat tgctcatgag ctggctggac
      361 tgagcctggc ggatgccgac ttgctgcgcc gggcgatgtc tcattttgac ccgggaaagc
      421 aaatgcagac tttgcaggag aaattcatcg ctggggcagc ggagcgaaag ggagtccctg
      481 cggatgtggc tgagcgcatt tgggagctga tggcggcgtt cgccggatac ggttttccca
      541 aggcgcacgc cgcctcgtat gcccaggttg cctggcaatc ggcgtggtgt aaagcccatc
      601 atccggcgat attcatggcg gctgtcctgg ctaattgggg aggatattac gggcagcgtg
      661 tttacctgac agaagcaagg cgcatggggt tgactctccg cccaccacat gttaacttcg
      721 cccggcgaga atttagcgtg gcctacgaaa agagccaacc ccttctctat atggggctgg
      781 atcaagtgcg agagctgacc cgccagacca tcgagcgaat tatccgcttg cgaccctttg
      841 cctcgctgga ggatttctta acccgcgtaa atcctcgccc gggggaaatc gataacctga
      901 tccgagcagg cgccctggat gtcttcggaa agatacccgc tctgttgcgc cgtttgaaag
      961 gagaaatcgt tcgcggggga cagctctccc tgtttgttag tgatgaggat gccgacgatt
     1021 ggtcattgga agagaaagtc gctgcccagg aagccattct tggggtgagt gtcatcgccc
     1081 acccgctgga gatggtaact gaccagattg ctgaagctgg ggcactcagt acgctgga
//
LOCUS       DBNHOH010000439         1423 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_858469, whole genome shotgun sequence.
ACCESSION   DBNHOH010000439 DBNHOH010000000
VERSION     DBNHOH010000439.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_858469"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="AC16V7_07720"
     CDS             complement(<1..610)
                     /locus_tag="AC16V7_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6257045.1"
                     /translation="MSNVAIRVEHLGKQYGLGGRQASITTFREILVDSARAPLRWLKG
                     ERKAEQNLFWALDDVSFEVKHGEAVGIIGRNGAGKSTLLKILSRITSPTRGRVDIFGR
                     VGSLLEVGTGFHSELSGRENIYLNGAILGMSRVEIGRKFDEIVDFSGIEKFLDTPVKH
                     YSSGMYVRLAFAVAAHLEPEILLVDEVLAVGDITFQQKCLGKM"
     gene            complement(612..>1423)
                     /locus_tag="AC16V7_07725"
     CDS             complement(612..>1423)
                     /locus_tag="AC16V7_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6257046.1"
                     /translation="VVIEPPKGWSSLELSELWKYHELLLFLTWRDIKVRYKQTMLGAA
                     WAILQPVLTMIVFSIIFGGLAKLPSDGIPYPIFTFTALLPWQLFAYALSQSSNSLVGS
                     QNLISKVYFPRLVVPFSSVLAGVVDFAIAFLVLVGMMAFYGIGLTPAALLLPLFVLLA
                     LASALAVGLWLSALNVQYRDIRYVVPFLTQFWMYATPIAYSSSLIPENWRWLYSLNPM
                     TGVVEGFRWVILGKSSLDVLSLGISACVVVLLLVGGLFYFKRMEANFADII"
BASE COUNT          393 a          397 c          327 g          306 t
ORIGIN      
        1 ccatcttacc taaacatttt tgttgaaagg tgatatcacc aactgctaag acctcatcta
       61 ccagtaatat ctctggttca aggtgagctg cgacagcaaa agccaggcgc acgtacatgc
      121 cgctggaata atgttttaca ggcgtatcga gaaatttctc aatgcctgaa aaatcgacga
      181 tttcatcgaa tttgcgcccg atctcaaccc ggctcatgcc caggatagcc ccgttgagat
      241 aaatattctc gcgcccgctc aattcagaat ggaaacctgt gcctacttcc agcagggaac
      301 cgacgcgccc aaaaatatcc acccgcccac gcgtcgggct ggtgatgcgc gaaaggattt
      361 tgagtaatgt gctcttccca gccccattgc gaccaataat ccccactgct tccccatgct
      421 ttacctcaaa cgagacatca tctaatgccc aaaagaggtt ctgctcggct ttgcgttcac
      481 ctttcagcca gcgcagtggt gctcgggcgc tatccaccag tatctcgcgg aatgtggtaa
      541 tcgatgcctg ccgcccgccc agcccgtact gcttccccaa atgttcaaca cgaatggcta
      601 cattgctcat tttatatgat gtccgcaaaa ttggcttcca tacgcttaaa gtagaataac
      661 ccgccaacta atagcaagac taccacgcag gctgaaatgc ccagggacaa cacatccaga
      721 ctgctcttgc ccaggatcac ccagcgaaaa ccctccacaa cgcccgtcat tgggttcagg
      781 ctataaagcc agcgccaatt ttccgggatc agtgaactcg aataagcaat tggagtggca
      841 tacatccaga actgcgttag gaacggcacc acataacgga tatcgcgata ctgcacattg
      901 agtgctgaaa gccataagcc taccgccaac gcgctggcca gtgccaggag cacgaagagg
      961 ggcagcagca gggcggcggg agtcaggcct ataccgtaaa aagccatcat tcccaccaat
     1021 accaagaagg caatcgcaaa atccactacc cccgccaata ccgacgaaaa tggcaccaca
     1081 agtcgcggga aatagacttt gctgatcagg ttttggctgc ccactaggct gttggaagat
     1141 tgggacagtg cgtaggcaaa tagctgccag ggtagcagtg ccgtaaacgt gaaaatcgga
     1201 tatgggatcc cgtctgaagg tagtttggcc agacctccga aaataatgct aaatacgatc
     1261 atggtcagca caggttgtaa tattgcccag gcagcgccca gcatggtttg tttatagcgc
     1321 accttaatat cgcgccaggt caagaataaa agtagttcgt ggtatttcca cagctcactc
     1381 aattcaagcg acgaccagcc tttgggtggt tcaataacaa cgt
//
LOCUS       DBNHOH010000440         2607 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1114403, whole genome shotgun sequence.
ACCESSION   DBNHOH010000440 DBNHOH010000000
VERSION     DBNHOH010000440.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1114403"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..981
                     /locus_tag="AC16V7_07730"
     CDS             85..981
                     /locus_tag="AC16V7_07730"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF012693.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR6257047.1"
                     /translation="MKRFIDKLRGSQQALSQEIPTRPIPKENLETISNSPHPRPEPPQ
                     LIVGCAQSTGVQRNNNEDALFTLTTTLTGDFRNTPFGLYIVADGMGGHQHGEIASGIA
                     IRSMASYVIRKLYSPLLSLKPNLPEESLLEIMQEGVQDAHSAIIKQVPGGGTTLTAAL
                     IVGNRMTIAHVGDSRAYLIGNNGKIEALTRDHSLVNRWVELGQITLEEAAVHPHRNVL
                     YRALGQGEPFDPDINSIQIPDTGYLLICSDGLWSVVPEEEIQAIVQKSKNPEQACQML
                     VEAANAAGGPDNITVILIRLHV"
     gene            990..2498
                     /locus_tag="AC16V7_07735"
     CDS             990..2498
                     /locus_tag="AC16V7_07735"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HIR6257048.1"
                     /translation="MEQLPDYYAQLGVSREASAEEIRRAYHRAARELHPDLNEDVNAN
                     DFFLQLQAAYEVLSDPDKRTEYDQSLPPLEEKTPAVGLNVLFSRPSLIKISEPQMIFV
                     MMELTAPPAAKAEPSPPLNVCLVLDRSTSMRGERMDTVKRTAIKLLRQLRQEDIISIV
                     TFSDRAEVLVSGGKLSNRIEIENKIKMIQPSGGTEIYHGLDAGFFEVRSRSRNNSTNH
                     IILLTDGRTYGDEAECLRIADQAAYSGIGISGLGIGSEWNDVFIDSIAARTGGSSLYI
                     SNPSEIEKALNEVFSGLRQIYAERVSFEFFAQPGVELHYAFRLSPQANPLKTSSPIRL
                     GRIPMEADLTFLLEFIVDPIDKTSDALTLIEGQISMDIPTRANTEHTIPVHLSGVISQ
                     ESSTEPPPTQLLKAVSQLTLYRMQERANQYLAEGQTDDASRFMKNLATHLFSQDQHDL
                     ARAALAEANNILHKQRLSETGKKRIKYGTRALLLPSGYTGQIDSPPGKGNSP"
BASE COUNT          695 a          706 c          615 g          591 t
ORIGIN      
        1 gacggtgtag tatacatcgg ttcaaattat catcacctct atgcacttcc tgcatagtag
       61 ttaaaaacag agaaggtaaa ctacgtgaag cgcttcatcg acaaattgcg cggctcccag
      121 caagccctga gccaggagat accgacgcgg ccaattccca aagaaaacct cgagacgata
      181 tcgaactcac ctcatccgcg ccccgagccc ccacagctaa ttgtaggttg tgcacaatca
      241 acaggagtac agcgcaacaa caacgaagat gccttattca cccttaccac caccctgact
      301 ggcgacttta gaaatactcc cttcggcttg tatatcgtgg ctgatgggat gggcggacac
      361 cagcatgggg aaatcgccag tggcattgcc atccgttcga tggctagtta cgttattcgc
      421 aagttatatt cccctctact gagcctaaaa ccaaatctcc ccgaagaatc gctccttgaa
      481 attatgcagg aaggcgttca agacgctcac tctgcgatca ttaagcaggt gccgggcggt
      541 ggcaccaccc tgaccgcggc attgatcgtc ggcaaccgta tgaccatcgc acatgtagga
      601 gatagccgcg catacctgat tggcaacaac ggaaaaattg aggcgctaac acgcgaccat
      661 tctttggtta atcgctgggt ggaactggga cagatcacac tcgaagaagc agctgtccat
      721 cctcaccgga atgtgctcta tcgagccttg ggtcaaggcg aaccatttga tcccgatatt
      781 aattcaatac aaatacctga cacgggttac ctgcttattt gctctgatgg actttggagt
      841 gtggtgccag aagaggaaat ccaagccatc gtacagaagt cgaagaatcc tgaacaggct
      901 tgccaaatgc tggtggaggc tgccaacgcg gctggtgggc cagataacat cactgtcatc
      961 cttatccgtt tgcatgtata acagccttta tggagcaatt acctgattac tatgcgcaac
     1021 tgggggtgtc tcgagaagcc tcagcggagg aaattcgccg tgcctaccac agagccgctc
     1081 gcgagctcca tcctgaccta aacgaggatg tgaacgcgaa cgactttttc ctacaacttc
     1141 aagccgccta tgaggtgcta tccgatccag acaagcgtac cgaatacgac caatctttac
     1201 caccgctgga agagaaaacg cccgcagttg gcctaaatgt cttgttcagc cgcccttcgt
     1261 tgatcaaaat cagcgagccg caaatgatat ttgttatgat ggagctgact gccccgccag
     1321 cagccaaggc agaacccagc ccccctctca atgtatgtct ggtgttggat cgctcgactt
     1381 ccatgagagg cgagcgcatg gatacggtca aaagaacagc catcaaattg ctccgccagc
     1441 tccgccaaga agacatcata tccattgtga ctttcagtga tcgggctgag gttttagtct
     1501 ctggcggaaa actctccaat agaatcgaga ttgaaaacaa gatcaagatg atacagccca
     1561 gtggtggcac cgaaatctac cacgggttgg atgcaggttt ttttgaggtg cgcagccgga
     1621 gcagaaataa ctccaccaac cacattatcc tgctcacgga tggtcgcacc tatggagacg
     1681 aagccgagtg cttgcgtatt gctgaccaag ctgcgtactc cggaatagga attagtgggc
     1741 ttgggatcgg ttctgagtgg aatgatgtat tcatcgatag catcgctgct cgcaccggtg
     1801 gcagtagcct gtatatctcc aatccgagtg aaatcgagaa agccttgaac gaggtgtttt
     1861 caggattacg gcagatatat gccgaacgag tgtcttttga attttttgct caacctggag
     1921 ttgaattgca ttacgctttt cggctctccc cacaagctaa ccccctgaag accagctctc
     1981 ctatccgttt gggcaggatc ccaatggaag cagatttgac atttttgctt gaatttattg
     2041 tagaccccat cgataaaacc agcgatgcgc tgacactcat tgagggacaa atttcgatgg
     2101 acattcctac tcgagccaat acggaacaca caattcctgt gcacctatcc ggggtgatta
     2161 gccaggaaag cagtaccgaa cctcctccaa cacaactgtt gaaggccgtc tcccagctta
     2221 ctctataccg catgcaggag cgggctaacc agtaccttgc tgaaggtcag acagatgatg
     2281 ctagccgttt catgaaaaac ctggccaccc atcttttttc tcaagaccag cacgatctgg
     2341 cacgcgctgc tttggctgag gccaataaca tccttcacaa gcaaaggctg agcgaaaccg
     2401 gcaaaaagcg cattaaatat ggcacacgcg ccctgctact tccctccggc tataccggtc
     2461 aaatcgattc ccccccagga aaagggaact ctccatgatc gcatgccctt cttgccagca
     2521 cgaggaatta tacggcgcac tcttttgcag cgaatgtggc actcaaatca catatcaaaa
     2581 tccatcgcca ccagagacag ctgtcta
//
LOCUS       DBNHOH010000441         7286 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1243395, whole genome shotgun sequence.
ACCESSION   DBNHOH010000441 DBNHOH010000000
VERSION     DBNHOH010000441.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7286)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7286)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7286
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1243395"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="AC16V7_07740"
     CDS             complement(<1..746)
                     /locus_tag="AC16V7_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257049.1"
                     /translation="MESQEIPKSEVIEQVYEWIYSLGALRAGLELRVWEKIAAGVDTA
                     DKIAAQEGWNLAGVRVLLDTICALKLLTRDGERYYLVPESAYYLIPGKPTYKGGIVEH
                     EINWEGNGKLAEAIRSGVRPVVYDATTADVVGLWIADYSRRWVYPEAYFDIDNKLWQS
                     LEIQAQEGLRVLDLACGPAPISMALARLNPGVCITWLDWEDVLQTALKVAVGLGIANQ
                     VVLMPGDLWSVDLGSDAFDVAYLGNVTHFF"
     gene            complement(759..1169)
                     /locus_tag="AC16V7_07745"
     CDS             complement(759..1169)
                     /locus_tag="AC16V7_07745"
                     /inference="COORDINATES: protein motif:HMM:NF024865.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6257050.1"
                     /translation="MEPADDLRAFLRDWIDKSHYDLTRVEKCWSDQEGALCIFAAPHE
                     WLEGRQAILDYYRAALPGSGEYRVIVDHLDAYCEGSVGWVAARPVFVLPNGDQVPARF
                     TAVLHREENIWKFVQYHVSVGVTDEEFFHKAQPG"
     gene            complement(1261..2511)
                     /locus_tag="AC16V7_07750"
     CDS             complement(1261..2511)
                     /locus_tag="AC16V7_07750"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012958.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HIR6257051.1"
                     /translation="MKKVYCLFLSVLVAVIMGSCTNSPSASVKGIITPGKKIGEMTAE
                     QGTPTLIRPYIWQFCENMPDVHTPNASTIDCDVPMMSGMTIQLGWLAKESKFASNWDA
                     MTWELFIDGYKIDLDTFDWYDSDYPAHGEANKSRIWYINLKNLSPGEHTLRMLWESKL
                     AVDDGMNVYQPGTYEHRVNFTVLEKAVYPVLPSTTIGQHPYTSEKADLDFLLYLPKDY
                     GKNPQQKWPLIVYLHGAPVRGSTLELLMQEPLPQQLNTENDFPFIVVSPLGDGEYEFW
                     AKDTMINQLFTLLDEIKTEYSIDTKRIYLTGNDMGGNGVWVIGLLNPEYFAALVPLAG
                     YASYPFEVPENICELKNVPVWAFHGERDPYVPAQVAQDLVDALIACGGDAKITLSRQM
                     KMDVPFKVYKDPKLYEWMLSQIQE"
     gene            complement(3139..3636)
                     /locus_tag="AC16V7_07755"
     CDS             complement(3139..3636)
                     /locus_tag="AC16V7_07755"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6257052.1"
                     /translation="MKTNIFAPDLFKDRVVLITGAAGGVGGATAELLGSLGAKLALTD
                     CKEEAVKEKAATLEALGVQADISSVADCQRVIATTLAYYGRLDALVNCAGVWVEGDSA
                     EATEEDWERCVNVNLKGTFFMCSKAIPALKKSRGAIVNISSDAGVVGNLGAAVMLDFG
                     ITAGY"
     gene            complement(3639..4421)
                     /locus_tag="AC16V7_07760"
     CDS             complement(3639..4421)
                     /locus_tag="AC16V7_07760"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6257053.1"
                     /translation="MHPLFSLEGQVALVTGAGSLQGIGFATAKLLAEMGAKVAVAATG
                     QRIHDRAAELLQVGHTARGYIADLTDRAATRSLVEAVESDFGKIDILVNNAGMTMEGA
                     PEEFSEFAELSDEAWDLSIARNLTTCYNVTRLVIPGMIGRRYGRIINVSSVTGPLVSN
                     RGEAAYSAAKAAMIGMSRSIALEVAEHNVTINNVAPGWVATGSQTDEEKIASAHTPFG
                     RAGTPTEMATMIAFLATPGASYITGQMMIVDGGNCLQESKGV"
     gene            complement(5078..5398)
                     /locus_tag="AC16V7_07765"
     CDS             complement(5078..5398)
                     /locus_tag="AC16V7_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009358741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HIR6257054.1"
                     /translation="MPKYLVQANYVGDGVKGLIKEGGSSRRAAVAKLFESVGGKVEAF
                     YYAFGETDLFVIAEIPDNVTAASLSLTVNASGAATTKITVLLTPEEVDAAAKKSPSYR
                     PPGP"
     gene            complement(5528..5797)
                     /locus_tag="AC16V7_07770"
     CDS             complement(5528..5797)
                     /locus_tag="AC16V7_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257055.1"
                     /translation="MMKARLTQSAPDWRDFAAFSGFIYAQAESCSQAESTPARQQRTQ
                     TVWQFHTRLLRNIPWIEFSAKSYKNHPLAQHYSCLFNHSVLDAMV"
     gene            complement(5794..6852)
                     /locus_tag="AC16V7_07775"
     CDS             complement(5794..6852)
                     /locus_tag="AC16V7_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR6257056.1"
                     /translation="MKPGYKKLFIVMVALILVACVATPSQSPTSTPVSAPTILSTPTP
                     TSVPTILPTPSTSVPLEERLLAAIDVKLPDELVFAKGFIWVKTDSGHVVQVDPATNSV
                     VGDIKVDTTSWQHDYCQGLGTDGENIWACSTRGNENFKTIDVVRIDPSSQTVTATVEV
                     GKIFDQYNMPFLSNHIWVLTDDGSKLVGIDTTTNQPSPAIDLGVRCFQVAATSSSLLV
                     TCKLDDLILRVDPESMQVTERRTFTRSPWNIVATEDGAWVGLGNAVVRLDPESLNPVA
                     TFSGLSGDKGLFATKEGLWVRLDEGFLYRIDPVSNQIVEQITSDQRFYNLGSVIVTSD
                     AIWTTAGDDDLLLRISLK"
BASE COUNT         1837 a         1895 c         1702 g         1852 t
ORIGIN      
        1 ctgaagaaat gggtgacatt gcccagatag gctacatcaa aagcgtcact accgagatcc
       61 actgaccaca ggtcgccagg cataagcacc acctggttgg ctatccccaa tcccactgcg
      121 actttgaggg cggtttgaag gacatcctcc cagtccaacc aggtgataca cacgcccgga
      181 ttcagccgcg ccagcgccat tgaaataggc gccggtccgc acgccaggtc cagcacccgc
      241 aacccttcct gcgcctggat ctcgagggat tgccaaagtt tgttgtcgat atcgaaataa
      301 gcctctggat agacccatct gcgtgaataa tccgcgatcc atagaccgac aacatctgca
      361 gttgtggcgt cgtacaccac gggtcgcacc ccgctgcgga ttgcctctgc cagcttgccg
      421 ttcccttccc aattaatttc atgttccacg atgccgcctt tgtaggtggg tttgccggga
      481 attaaataat aggcggactc cggtaccagg taatagcgct ccccatctct ggtcaacagc
      541 ttgagagcgc agatggtatc cagcaggacg cgtaccccgg ccaggttcca gccctcttgc
      601 gccgcgattt tgtctgcggt gtccacgccc gcggcgatct tctcccatac ccggagttcc
      661 aacccggcgc gcagcgcgcc gagactgtag atccattcgt agacctgctc gatcacttcc
      721 gattttggta tttcctgcga ttccatattc cctccgatct atcccggttg agccttgtga
      781 aagaactcct cgtctgttac acccaccgag acgtggtact ggacgaattt ccagatgttc
      841 tcctccctgt gcagtacggc ggtgaaacgc gcaggaacct ggtccccatt gggaaggacg
      901 aagactggtc gagctgccac ccagccgaca ctgccttcgc aatatgcgtc caggtgatcc
      961 acaatcaccc ggtactcccc gctgcctggc agcgctgcgc ggtaataatc caggatcgcc
     1021 tgacgcccct ccagccattc gtgtggggca gcaaagatgc acaatgcgcc ttcttggtca
     1081 gaccagcatt tttccaccct ggtaaggtcg tagtgggatt tgtcgatcca atctcgcagg
     1141 aaagccctta gatcatctgc tggttccatc tttcgtaact cttgctataa acgttcggct
     1201 tacctcccga tactttcatt ggggacggca atacaaacgt gtaggtgaac catcccaata
     1261 tcactcttgg atttgtgaca acatccactc ataaagtttg ggatctttgt aaactttgaa
     1321 gggaacatcc attttcattt gtcggctgag agtaattttt gcatcccctc cacatgcaat
     1381 tagagcatcc accaaatcct gagctacctg agccggaaca tatgggtctc tttccccgtg
     1441 gaatgcccag acaggaacat tttttaattc gcagatgttt tcagggactt caaaagggta
     1501 gcttgcatag cctgcaagtg gcactaaggc cgcaaagtac tctggattaa gcaagccaat
     1561 cacccaaaca ccattaccgc ccatatcatt gccagtgaga tatattctct tggtgtctat
     1621 agagtactca gtcttaattt catccaataa agtgaatagc tgattgatca tcgtgtcttt
     1681 agcccagaat tcatactccc catcccccag tggagatacg acgatgaagg ggaaatcatt
     1741 ctctgtattt agttgttggg gtagtggttc ttgcatgagt agttcaagag tggagccgcg
     1801 tacaggcgca ccgtgaaggt aaacgatcaa aggccatttc tgctgcgggt tttttccata
     1861 gtccttcggc aggtaaagca ggaaatcaag gtctgctttc tcagaggtat aagggtgctg
     1921 accaattgtg gtcgatggga gtaccgggta aacagctttt tctagtacag tgaaattcac
     1981 cctgtgctca tacgtaccag gttggtagac attcatgccg tcatcgaccg cgagtttcga
     2041 ttcccataac attcgaagag tgtgctcgcc aggagacaga ttttttagat ttatatacca
     2101 aatgcgactt ttattagcct ctccatgcgc aggataatca gaatcatacc agtcaaaagt
     2161 gtctagatcg atcttataac catctataaa taattcccat gtcatcgcat cccagttaga
     2221 tgcaaatttc gattctttgg cgagccagcc aagctgaata gtcattcctg acatcatcgg
     2281 gacatcgcaa tcgattgtag atgcgtttgg tgtgtgcacg tcaggcatat tttcacaaaa
     2341 ctgccatata tatgggcgta ttagggttgg tgtaccttgc tctgcggtca tttcaccgat
     2401 ctttttccca ggtgtaatta tccccttgac tgaggcagag ggactatttg tgcatgatcc
     2461 cataatgacg gcaaccagaa cagacaggaa caggcaataa acctttttca tgattgcctc
     2521 ctttatatat agagaacaga ttgagctgat gttaggtatg cttgttctgc caacgatagg
     2581 caaaacttgt gatttactcc acaaactcat tctactgcaa aaaaggataa aatcaagcca
     2641 gtaaatgtga actgcccaac tattggatat actgcatatt tgcagcatat cttaccaatt
     2701 gggcgatttt aaggattcac cagagatgat atttttaaga acggttatct ttactataat
     2761 gtactttaca atatttttct tgcgttatca acagattcgg caatgaattc ttaaaacaat
     2821 tacgcccagt gagaactggg cgtacgatct ctatgacatg ttccgccctt ccagcgcgcg
     2881 gacgattaga gatcttgccc gcagctatgc aaccgctaag acactgaaat agggccaatg
     2941 ttcaaggccc tcagccgcgg gtcgaggaca acccgtaagc catctcctac caggctctac
     3001 cccgtcgaga aaagcactac ggcggcgctc accgggatgt aggtgtacca atattaaagg
     3061 atcgccaatc atccagtcgc gagaggtgct cagcatgtcc cccgacaaac aaaccgattt
     3121 gttgatgaga aagcagtttc aatatcccgc cgtgatgcca aaatcgagca tcaccgccgc
     3181 gcccaagttt cccaccacac ctgcatccga actaatattc acaattgcgc cgcggctttt
     3241 ctttaacgca gggatggctt tactgcacat aaagaatgtg cctttgagat ttacgttgac
     3301 acaacgctcc caatcttcct cggttgcctc ggcgctatcg ccctccaccc aaacccctgc
     3361 gcaattaaca agcgcatcca agcgaccgta ataggcaagt gttgttgcga tcacgcgttg
     3421 gcaatccgcc acggatgaaa tatctgcttg aacgcccaac gcttcgagag tcgcggcttt
     3481 ctctttgacc gcctcctctt tgcaatcggt gagcgcgagt ttggctccca aactccccaa
     3541 caattcagcc gtcgccccac ctactccgcc ggctgcgcct gtaatcaaca ccacacgatc
     3601 cttgaagaga tcaggagcaa aaatatttgt tttcatgatt acactccttt gctttcttgc
     3661 agacagttgc cgccatccac gatcatcatt tgcccggtga tataactcgc acctggtgtt
     3721 gcaaggaatg cgatcatggt tgccatttca gtcggagtac cggcgcggcc aaatggagta
     3781 tgagctgaag cgattttttc ctcatccgtt tgtgagcctg ttgccaccca gcccggagct
     3841 acattattga tcgtgacatt atgttcagcg acctccaatg caatacttcg gctcatgccg
     3901 atcatcgccg cttttgcagc gctatacgcc gcttcgccgc gattgctcac cagcggaccg
     3961 gtcacagatg agacattgat aatgcgtcca tagcggcgac cgatcattcc cggaataacg
     4021 aggcgggtca cattgtagca agtggtcagg ttgcgcgcga tagataaatc ccaagcttca
     4081 tccgagagtt ccgcaaactc actgaactcc tccggcgctc cttccattgt catccctgca
     4141 ttgttaacga ggatatcgat ttttccaaaa tcagattcga ccgcttcgac cagtgacctg
     4201 gtagctgctc tgtccgtcag gtcggcgatg tacccgcggg cggtatggcc cacctgtagc
     4261 aattcggcgg cgcggtcgtg aatgcgttgc cctgtcgcgg caacagcgac ctttgctccc
     4321 atctccgcca gcagtttggc ggtggcaaac ccaatgccct gcagactgcc cgcgccggtc
     4381 acgagcgcaa cctgcccttc aagcgaaaat agtggatgca tgttcaagcc tcttgttgtg
     4441 agatttcgtg cctcatacac gattttcggt agtggatgaa aagtaagtga gtaatgccta
     4501 tcacagcccg agagacacta ccctcccact tgctttgtga ccactagcta aattttgaac
     4561 taccaagaat gaacggtgag atgggaaatt ataatgccga ttccagattg gaaaatgata
     4621 gatccctgtg cgttctttag ttaagaatca cgcgtttgag taactgaggt tcgacattcc
     4681 tgcgggtcct aacttataag atgttcaggc aaagatttat tgcttgtcta accgctacaa
     4741 tttaccttta ccgcttggtt atatcctgcc aagggcttgg gggctccatc agtgtagatg
     4801 atttccttcg ccatttgtaa atacctgcgt ttttgataaa tgaccgccaa acgacttacg
     4861 taccctgcgc aggggcggac ggcggtacac cgtccaatga gaataaaact aaggcatgga
     4921 aaatgcctga aaacggcgca gaatcccgcc agtcaggtgc acgctttgtt ggacagggtc
     4981 ttggaaaaac aaagcatctt tttgcggtca tatgccgccg acctcacaac ttaggtcggc
     5041 ggcaacttta cctattatcg ttacgaaagg aaataactta cggtccgggc gggcggtaac
     5101 taggtgattt tttcgccgcc gcgtcgactt cttcaggagt taatagtaca gtgattttgg
     5161 tagtggcagc tcctgaggcg ttaactgtca gagacagaga cgcggcggtg acgttatcgg
     5221 gtatttctgc aatgacaaaa agatcggtct ctccgaaggc gtaatagaag gcttccacct
     5281 ttccgccaac tgattcgaac aatttcgcga ccgctgcgcg tcggcttgaa ccgccttcct
     5341 tgatcaatcc tttgactcca tcaccgacat agttggcttg aacgagatac tttggcattt
     5401 tattctcctt tagtaataag tgtgctcctc cgagaaggca cattgacgga taacgataaa
     5461 caattaatgg ttggaacctt gacttatagg cacctcctta tagatctcac accttcaact
     5521 tcccctatta gaccatagca tcgaggacag agtggttgaa taaacaagaa tagtgttgtg
     5581 ccaatggatg gtttttataa ctcttcgcag aaaattcaat ccaaggaata tttcttaaaa
     5641 gccgcgtatg gaactgccaa acggtttgcg ttctctgctg gcgggcgggc gtggattctg
     5701 cttgggagca ggattctgct tgggcgtaga taaagcctga aaatgccgca aaatcccgcc
     5761 agtcaggtgc actctgtgtt aggcgcgctt tcatcatttg aggctgattc gaaggagcaa
     5821 atcatcatcg cccgcggtgg tccagatggc gtcggatgtg acaataacac tacccaagtt
     5881 gtaaaacctc tgatcggacg tgatctgttc aacgatctga ttgctgaccg ggtcaattcg
     5941 gtagaggaaa ccttcatcca gccgcaccca aagcccttct ttcgtggcga aaaggccctt
     6001 atcgccagac agcccggaaa atgtagcaac gggattgagc gattctggat ccaggcggac
     6061 gacggcatta cccagaccaa cccatgctcc atcctccgtt gcgacgatgt tccaggggga
     6121 acgagtaaat gtccgtcgct cagtgacctg catgctttcc gggtcaactc gaagaataag
     6181 atcatccaat ttgcaagtga ccagcaaaga gctgctcgtt gcggctactt gaaaacatcg
     6241 cacaccgaga tcgatcgccg gtgagggctg attggttgtg gtatcgattc cgaccagttt
     6301 ggaaccgtcg tccgtaagca cccagatgtg attgcttaga aacggcatat tgtactggtc
     6361 aaaaatcttt cctacctcga ccgttgcagt gactgtttgc gagctgggat caatacggac
     6421 aacatcaatg gttttaaagt tttcattacc cctggtagag catgcccaaa tattttctcc
     6481 atctgtgcca agcccttgac aatagtcatg ttgccagctg gttgtatcca ctttgatatc
     6541 gccaaccacg ctatttgtgg ccgggtcaac ctggaccaca tggccactat ccgtcttgac
     6601 ccagataaaa cctttagcaa agacaagctc atcaggtaac ttgacgtcga tcgctgccaa
     6661 taagcgctct tcaaggggca cgctcgtcga aggcgtgggc agaatcgtcg ggactgaggt
     6721 aggcgtaggc gtggacagga tcgttggagc tgaaacgggc gtagaagtag gcgattgaga
     6781 aggcgtagcc acacaggcca ctaatatcag tgcaaccata acaataaaga gttttttgta
     6841 tccgggtttc ataacagtaa atcctctaaa cacttttcaa aagatggatg aaaattcttg
     6901 cgtatttatt tcaacacgca acaagtcgcc agcgaccaac tttgctttag aagcgctagc
     6961 ttcctaatca accaaattat agaattgtaa agatattaca ggaaaatagc ttacccccac
     7021 gagtggattt taccagattc tttcgaaaaa aaacatattt attgaggtac ttagggtcat
     7081 atgtgggtga tcaccagtgt catttctgtt gaccacctcg ttgatttatt gcagaatcca
     7141 acccaactta accgaataag gcgatgttgg aaacgagtaa gcctgagcta aactgttagc
     7201 gaacccggga gtgatgagag cttgagtaga ccatcaggct ggaaatcctt ccggagccgc
     7261 aaaattagcg atcagccaac tgatgg
//
LOCUS       DBNHOH010000442         6987 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_858734, whole genome shotgun sequence.
ACCESSION   DBNHOH010000442 DBNHOH010000000
VERSION     DBNHOH010000442.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6987)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6987)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6987
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_858734"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="AC16V7_07780"
     CDS             <1..283
                     /locus_tag="AC16V7_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018153127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidinotransferase"
                     /protein_id="HIR6257057.1"
                     /translation="HLKSSVNYAGKNTLLICEDFAGRGEFQGYRTIVVPQTEAYAANT
                     LWANDHLITPAGFPETRKRLMALDLPIIELDVSEAHKMDGGLTCMSLRF"
     gene            315..785
                     /locus_tag="AC16V7_07785"
     CDS             315..785
                     /locus_tag="AC16V7_07785"
                     /inference="COORDINATES: protein motif:HMM:NF024052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HIR6257058.1"
                     /translation="MTEHLTPEKLHVTYIDDVRDDGPLAPRAYTLTHSDTTGDLFLTI
                     GLRYNLPQISGLYTRFMRDEVLAEWRQEEQLTLHVHCHVSGGLVFGSSGYRDSIFRYH
                     LPMVLEAFRYGDQKLVEAHPQLAQAPIMVHFHAREDKFDKTEQWGVFDEYQISD"
     gene            864..1892
                     /locus_tag="AC16V7_07790"
     CDS             864..1892
                     /locus_tag="AC16V7_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR6257059.1"
                     /translation="MKAVRMVDVNLPLQMFDIPIPALGEGDILVRIRAAGICHSDAHY
                     RAGKSPVRPLPMTLGHEIAGIVEEVGKRVTSVKVGDRVCLHYLLSCGECSYCRIGSEQ
                     FCTQGSMLGHYSDGGYAEYIAVPERNAVHLSDEISFEQGAIMMCSSATSFHALRKSRL
                     RAGETVAIFGIGGLGISAVQLASLFGALEVFAVDINPEKLKLVERYGAIPINATLNDP
                     VAEIRRLTHNKGVDVALELIGLSQTMKQAVRSLAFMGRAVIAGISDKPLEIDTYWELL
                     GKETEVIGTNDHLLSELPTIIEFARQGKLDLSGVITRTVPLEADHINRALDNLEKFGD
                     DIRTVIIS"
     gene            1958..5674
                     /locus_tag="AC16V7_07795"
     CDS             1958..5674
                     /locus_tag="AC16V7_07795"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6257060.1"
                     /translation="MNEQEQLEHAIAALEAQRSLLGDSVVDAALASMRQRLAILRSPP
                     LAEQRKTVTILFADLVDFTAMSEQLDPEDVREIVNAYFTRCTEAIEMYGGNIEKFIGD
                     AVMAVFGLVEAREDDPENAVRAALNIRQALSELNQEVQARWEMRLRMRIGIHTGPVVV
                     SLLSERKGMDFVVVGDAVNLASRLQSVAPADGILISHSTYRHVRGNFNVQVIEPIRIK
                     GKSESIQAYVILEAKSRPFRLETRGVEGIETRMVGREAEFQLLSDALDQMVRQGQNRI
                     VTLVGEAGVGKSRLIYEFINWLELLPENFTFFKGRAGPAMQNAVFSLLRDLFSFRFQI
                     QDSDPPQVVQEKMERGIYWAYETLPKLEQGGDHPAKQLSIQEIQKRAHFIGHLVGFDF
                     LDSPYLRVDKHDARYLRDRAMVYLADYYRTLAVYRPIVIVLEDLQWADDNSLDVFSRM
                     TSILPQSDSSHGVLVLCAARPDFFTRLPGWDEIQPLNVRIDLQSLDRVTCIELVDEIL
                     QKIPRVPAELRELVIANTDGNPLYIEEFIKMLIEDQVIVKADEEWQIDLAQLTSIHIP
                     PTLTGILQARLDSLPVGERLVLHRASVIGRVFWDAAVEYLAENERSEDMPISAPTAGL
                     LDRLRSREMVDQRKESVFEKTREYLFRHALFRDVAYESVLKRQRSLYHAHAARWLEAV
                     TQRSGRADEFSLLIADHYDRANEAQPAAKWYYQAGLQAAEHFANREAIRYFNRCLELL
                     ADGSPEDRFPAILAREKVFELLGERQLQHEDLESLTRLVDEMTPGKETDTRRVQVLLR
                     RSRLSEATSDNVASIEYARRALKHAQIASLDESMASCYLLWGSALWRQGKYVEARQPL
                     EKALEIARRVSIPNLEADILRNLGIVNAVLSNYTEARLYYEQSLAIKRELGDLRGESA
                     TLNSLSMLVYDLGDYLQARQYLEQSLNIKREIGDRRGEEIAMNNLGLVAYSEGDPVAA
                     RRYMGEALSMSQEILDLEGQTTALAVLGLIATYQGDYAAAQDYLENSLNLFREQVNDV
                     QGESEALSYMALLQNRLGNQEVAQEYATRALELARDVGAWREQFNALLFLGHALLALN
                     RTEEAIQAYQQALDLPDKERQTSPHLEAVAGLGRAWLEKWQVNSGISAGMANDALEQA
                     RNCVEIILDHLATKPLQDVHEPARIYLTCYNVLHASRDARASWVLEQAYHWLNDRAST
                     FQDEALKRLFLDNIPANRIISAEWETAHQLEDKP"
     gene            5671..6936
                     /locus_tag="AC16V7_07800"
     CDS             5671..6936
                     /locus_tag="AC16V7_07800"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR6257061.1"
                     /translation="MNRDLIRQVPLFSQLPDAELDYLAQTLQYCERGPDTILFREGDL
                     SQCFMVILDGQIEIVKALGTPEETVLTVEGPGEFLGEISLFLPDRNRSASARTLTHVQ
                     LFELSPAGFHTLLHRNPSLALPIMQVLTQRLRNSENTTIRDLQQKNIQLSEALQELHA
                     AQEQLIEKEKLEHELQVARQIQERFLPKELPSKPGWEIAAYWQPARAVGGDFYDFVVF
                     PNGQLGIVVGDVSGKGVPAALIMATTRSVMRAVTEHHFSPGSILERVNDLLNPDMPSN
                     MFVTCVIAVLEPSSGHLRLANAGHDLPHHLALDGSLELHATGMPLGLLPGMRYDEIET
                     TLSDGDSLLFYSDGLVEARDTQGEMYGFPRLRKFLSTWAQQKMASLTPDGASELLIRD
                     LLVSMNAFTGPVLEQDDDVTFVTLRRLQI"
BASE COUNT         1618 a         1779 c         1934 g         1656 t
ORIGIN      
        1 gcatctgaaa tccagtgtga attatgcggg caaaaacacc ttgttgatct gcgaggattt
       61 tgccgggagg ggggaatttc aggggtatcg tacaattgtt gtgcctcaga cagaagctta
      121 tgcagccaac actctgtggg ccaacgatca tctgatcacg ccagcgggtt ttcccgaaac
      181 caggaagcgg ctgatggctc tcgatctgcc gatcatcgag ctggatgtta gcgaggcgca
      241 caagatggat ggtgggctga cttgtatgtc tttgcgtttc tagcatacaa tttggttcaa
      301 ttcacaggtt agtcatgacg gaacatctca cgccagagaa actgcacgtc acttatatcg
      361 acgacgtccg agacgatgga ccgcttgctc ccagggcgta cacattgact cactcggaca
      421 cgacagggga tttgttcctc acaattggcc tgcgttacaa cctgcctcaa atatcaggtt
      481 tgtatacgcg gttcatgcgg gatgaggtgc tggctgaatg gcggcaggaa gaacagttaa
      541 ccctgcacgt tcactgccat gtcagtggag gattggtgtt cgggtcatcc ggttaccggg
      601 attcgatctt caggtaccac ttgccaatgg tcttggaggc attcaggtac ggcgatcaaa
      661 aattggttga agcacatccc caattagccc aggcgccgat catggtgcac tttcatgcac
      721 gcgaggataa atttgataaa accgaacagt ggggggtatt cgatgaatac cagatctcag
      781 attgatttgt tagtgctgtt tatttccctg tccatttcta ttatgggcag gcaataattc
      841 cctagaaagg attacgataa actatgaaag cagtccgcat ggtcgatgta aatctccctt
      901 tgcagatgtt tgacataccc atacctgcgc ttggcgaagg cgatatcctg gtacgcatac
      961 gcgcggcagg aatttgccac tcggatgcac attatcgagc tggaaaatcg cctgtgcggc
     1021 ctctgcccat gactctgggt cacgagatag ccggtatcgt ggaagaggtt ggcaaacgtg
     1081 tcacatccgt gaaagtggga gaccgtgttt gcttgcatta cctgttgagt tgcggcgagt
     1141 gctcctattg ccgtattggt agcgagcagt tttgcacgca aggctccatg ttggggcatt
     1201 attcggatgg tggttacgcc gaatacattg ctgttcctga gcgcaatgcc gtccatctgt
     1261 cggatgaaat ctcgtttgag caaggcgcaa ttatgatgtg ctcgtcggca acatcatttc
     1321 atgcgctgcg caagtcgcga ctcagggcgg gtgagactgt agccatcttt ggcattggtg
     1381 gtttaggcat atcggcagtc cagctagcat ccctcttcgg cgcgctggaa gtctttgcgg
     1441 ttgatattaa tccagaaaaa cttaaactgg tggaaagata tggggcgata ccgatcaatg
     1501 ccacgttgaa cgatccggtt gctgagatcc gccggcttac ccacaacaaa ggtgtggatg
     1561 tcgccctgga gctcatcgga ctttcgcaga cgatgaagca ggcagtgcgc tcgctggcat
     1621 tcatgggccg ggcggtaata gccgggatca gtgataagcc gttggagatc gatacctatt
     1681 gggagctgct tgggaaggaa accgaggtca ttggcaccaa tgaccacttg ctctccgagc
     1741 tgcccactat catcgagttt gcccgccagg gaaaattgga tctatcgggg gttatcacac
     1801 gcacggtccc gctggaagct gaccacatca accgtgcttt ggataatctc gaaaaatttg
     1861 gtgatgatat tcgaaccgtg attatatcgt gatcagcccg gctatttact gacccaggaa
     1921 ggctgatctc atatataatt gatctatata ctatcaaatg aatgagcaag agcagttaga
     1981 acatgccata gcagctctag aagctcagcg ctctttactg ggggattctg tggttgatgc
     2041 tgcgctggct tcaatgcgcc agcgtctagc tattctgcgg tcacctcccc ttgccgagca
     2101 gcgtaaaacg gtcactattc tcttcgccga cctggtcgac ttcaccgcca tgtccgagca
     2161 actcgacccg gaggatgtgc gcgagatcgt caacgcatat ttcacgcgct gtaccgaagc
     2221 gatcgaaatg tatgggggaa acatcgaaaa gttcattggt gatgcggtga tggcggtctt
     2281 tggcttggtt gaagcgcgcg aagacgatcc tgagaatgcc gtgcgcgctg ccctaaacat
     2341 tcgccaggcg ttgagtgagt tgaaccagga ggtgcaggcg cgctgggaga tgcggctgcg
     2401 catgcggatc ggtatccaca ctggtccggt ggtggtcagc ctgctcagtg agcgcaaggg
     2461 aatggacttt gtggtggtcg gcgatgctgt taacctcgct agccgtctgc aatcggtggc
     2521 tccggcagat ggtatcttga tctcccacag cacgtaccgg cacgtgcgtg ggaacttcaa
     2581 cgtgcaggtt atcgaaccaa ttcgcataaa aggcaagagc gagtctattc aggcctatgt
     2641 gatcctagag gcaaagagtc gtcccttccg cttggaaaca cggggcgtgg aaggcatcga
     2701 gacgcgcatg gtcggtcgtg aagccgagtt ccagctactc agtgatgcat tagaccagat
     2761 ggtcagacag ggtcaaaatc gtattgtcac gctagttggc gaagcgggtg tgggaaaatc
     2821 gcgtttgatc tacgagttca tcaactggct tgagctgcta cctgaaaatt tcaccttttt
     2881 caaaggccgc gccggcccgg caatgcaaaa tgccgtcttt tctttgctgc gcgatttatt
     2941 ctcctttcgc ttccagatcc aagacagcga cccgcctcag gttgtgcagg agaaaatgga
     3001 gcgcggcatc tactgggctt acgaaacact ccccaaatta gagcaaggag gagaccaccc
     3061 agccaagcaa cttagcattc aagagattca gaagcgcgcc cacttcattg ggcatctcgt
     3121 tggattcgac tttctggaca gcccatattt gcgagtagat aaacatgatg ctcgttatct
     3181 tcgtgaccgg gcgatggttt acctggccga ttattacagg accctggcgg tttaccgtcc
     3241 gatcgtcatt gtgctcgaag acctgcaatg ggcagatgac aactccctgg atgtcttctc
     3301 acggatgact agtattctcc cccagtccga ctcctcgcat ggcgttttgg ttctgtgtgc
     3361 tgcgcgcccg gattttttta cgcgcctacc tggttgggat gaaatccagc cgctcaatgt
     3421 tcgtattgac ctgcaatcat tggaccgcgt aacgtgcatc gagttggtgg atgaaatcct
     3481 gcaaaaaatc ccccgggtac ctgctgagct tcgcgagctg gtcattgcca acacagacgg
     3541 taacccgctt tacatcgaag aattcatcaa aatgctcatt gaggaccaag tcattgtgaa
     3601 ggctgatgag gaatggcaga tagatctggc acagctcaca tctattcata tccctcccac
     3661 actgacgggc attcttcaag ctcgactgga cagtctgccg gtaggcgagc gattagtttt
     3721 gcaccgtgct tcagtaattg gtcgcgtctt ttgggacgct gccgtagaat atctggcaga
     3781 gaatgagcga tctgaggata tgccaataag tgcgccaacg gctggattac tcgaccgatt
     3841 gcgctctcgc gagatggttg atcagcgtaa agaatcagtc tttgaaaaga cgcgtgaata
     3901 ccttttcagg catgccctat tccgtgatgt ggcgtatgaa agtgtgctta aacgccaacg
     3961 tagcctatac catgcgcacg ccgcccgctg gttggaggcg gtcacccagc gcagcggacg
     4021 tgctgatgag ttctctctcc tgatcgctga ccactatgac cgcgccaacg aagcacagcc
     4081 agctgcgaaa tggtattacc aggcaggttt gcaggctgct gagcatttcg ccaatcgcga
     4141 agcgatccgt tattttaatc gctgccttga gttattggcg gatggcagtc cagaggatcg
     4201 tttccccgca attctggcac gcgagaaggt cttcgaatta ttgggcgagc gccagttgca
     4261 gcatgaagat ctggaaagcc tgactcgcct ggtcgatgaa atgaccccag gtaaagaaac
     4321 agatacccgc cgcgtccagg tcctgctgcg ccgctctcgt ttgagcgagg cgacgtcaga
     4381 taatgtcgcc tccatcgagt atgctcgccg agctttaaag cacgctcaga ttgctagcct
     4441 agacgagagt atggcgtctt gctacctcct gtgggggtcc gcactttggc gacagggtaa
     4501 atatgtcgaa gcccgccagc ctctcgaaaa agccctcgag atcgctcgca gggtaagcat
     4561 acccaatctg gaagcggata tactgcgtaa cctcggtatt gtcaacgcag tgctaagtaa
     4621 ttacaccgaa gcgcgcttat actatgagca atcgctggcg ataaaacgcg agctggggga
     4681 tttgcgcggg gagagcgcaa cgctgaatag cttgagcatg ttggtttatg atttaggcga
     4741 ctacttgcag gcgcgccaat acctggagca aagcctgaac atcaagcgcg aaatcggcga
     4801 ccgacgcggc gaggaaattg ccatgaacaa tttgggcttg gtcgcttata gtgagggcga
     4861 tcctgtggct gcccgccgat acatgggaga ggctttaagc atgtcccagg aaattcttga
     4921 cctggaggga cagaccacag ccttggcagt tttggggctg attgccacct atcaagggga
     4981 ttatgccgct gcgcaagatt acctggagaa ctcgttgaat ctcttccgtg aacaggtgaa
     5041 tgatgtacaa ggcgagagcg aagccctcag ttacatggcc ttgcttcaaa atcgcctagg
     5101 aaaccaggaa gtggcgcagg aatatgcgac ccgcgccctc gaacttgcgc gcgacgtggg
     5161 agcttggcgt gaacaattca atgcccttct ctttctcggt cacgccttgc tcgcgctgaa
     5221 ccgcacggaa gaagccatcc aggcttatca gcaggcactc gatctgcccg acaaagagag
     5281 gcagaccagt ccacatctcg aagcggttgc cggactggga cgtgcctggc ttgagaaatg
     5341 gcaggtgaat tcgggcattt cagcaggtat ggcgaacgac gccctggaac aggcacgaaa
     5401 ctgtgtggag attatccttg accacctggc gaccaagcct ctgcaggatg tccacgagcc
     5461 agcccgcatt tatctgacct gctataacgt gttgcatgcc agcagggacg cgcgggcatc
     5521 ctgggtctta gagcaggcat atcactggct gaatgacaga gccagcactt ttcaggatga
     5581 ggccctgaag cgccttttcc tggataacat acccgctaac cgtataatat ccgctgaatg
     5641 ggaaaccgct caccagcttg aggacaagcc atgaatagag acttgatccg ccaggtgcct
     5701 ctcttttctc agttaccaga tgcagaattg gactacctgg cacaaaccct acaatattgc
     5761 gagcgtggtc cagatacgat cctttttcgt gagggtgacc tatctcaatg ctttatggtg
     5821 atcttagacg gacagatcga aattgttaaa gcgctgggca cgccggagga aaccgtgctg
     5881 acggtggaag gtccgggtga attcctgggt gagattagcc ttttcttgcc ggatcgaaat
     5941 cgctctgcca gcgcacggac cctaacccat gtccagttgt tcgagttatc tccggcaggt
     6001 ttccacaccc tcctccatcg caatccatcg ctggcactcc ccatcatgca ggtattgacc
     6061 caacggttgc gcaattcaga gaataccacc atccgcgacc tgcagcagaa gaatatccag
     6121 ctctctgagg cactccaaga actgcacgcc gcacaggagc aattgattga gaaagaaaag
     6181 ctcgagcacg agttgcaggt tgcccgccag atacaggagc gtttcctgcc taaagagttg
     6241 ccttctaaac ctggttggga gatcgccgcc tactggcagc cggcgcgcgc ggtgggcggc
     6301 gatttctatg atttcgttgt ctttcccaac gggcagcttg gtattgtggt gggtgatgtg
     6361 tccggaaaag gcgtcccggc tgcgctgatc atggcgacca cgcgcagcgt gatgcgcgct
     6421 gtcaccgaac atcactttag tcctggaagt atcctggagc gggtcaacga tctactgaat
     6481 ccagatatgc cgtcaaatat gtttgtcacc tgcgtgattg ccgtgctaga gccgtccagc
     6541 ggtcatctgc gcctggctaa tgccggtcat gatctacctc atcatctcgc actagatgga
     6601 tcgctcgagt tgcatgcaac tggcatgccg ctaggcttgc tgcctgggat gcgatacgat
     6661 gaaatcgaaa ccacgctctc cgatggcgat tcactgctct tctacagcga tggattggtt
     6721 gaagcgcgcg atacgcaggg cgagatgtat ggtttcccgc gcttgcgtaa atttctgtct
     6781 acatgggcgc agcaaaaaat ggcttcgcta actcctgacg gggcgagcga actgttgatc
     6841 cgcgacctcc tggtaagtat gaatgctttt accggtcccg tgctggagca agacgacgac
     6901 gtgaccttcg tcactttacg taggttgcaa atttaattcg ggggatacac aatgccaatc
     6961 tcagaaaatg agcgcttcaa ttgaagg
//
LOCUS       DBNHOH010000443         2526 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_89277, whole genome shotgun sequence.
ACCESSION   DBNHOH010000443 DBNHOH010000000
VERSION     DBNHOH010000443.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_89277"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..>2526
                     /locus_tag="AC16V7_07805"
     CDS             130..>2526
                     /locus_tag="AC16V7_07805"
                     /inference="COORDINATES: protein motif:HMM:NF037660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR6257062.1"
                     /translation="MKDSLLLTKLYIPSLSPRVVHRPHLISRLNEGLARGCKLTLLSA
                     PVGYGKTSLISDWLSTLNSRAAWLSLDTEDDDPLRFWVYVIAALQTIQPELGQTALTA
                     LQTAQMPPIENMLIDLINQISTLSDKIILVLDDTHLIETTEIHQSLNFLLDHLPPQIH
                     LVFITREDPPLPLPQMRAKGQISELRIKDLRFTMEESAEFLNHKMGLNLHPKGITALG
                     QRTEGWAAGLQMAALSLHELDDPAAFIEVFAGDNRYVADYLISEVLDRQPDHIRHFLL
                     QTAIVDRFTSSLCDALIGDTSHQSSRIIEKIEALGLFIMPLDHVRQWYRYHQLFADLL
                     RYRLRQDDPSRFMDLNRTASRWYEQQGLIDEAVKYGLVGEDHEYVAELIEISGLAMFG
                     RNQFARLQNWISALPEKIIQKHPYLSVLLVWVGSLTGQSDLARRHLALAEKNLSSAPA
                     DQHAEIISHVALLRAYATRTSGDLDSSIKHAQEALSYLPENNVFLDCTIHLNLGGNYW
                     LKGNFPAVEGPLKHAISFIDIPEVEYPVLAAAGFLANAYLQQGKLHNAEALCKKIVER
                     NSYHAHPAAAYVLLELGELFFERNDLDGALEVLSKTIQIGEIADKVVNVIRARQLLAR
                     VYQALGNQEEVANLMIQADELFKQTSPRYQVMHQIEYEYYRVRCLLFQQSTWGALQWA
                     DDYAKRREPVNNPWALLNELVYAHVLLIDRRPDQALSILKPCEDSARSYGARGWVVQN
                     LALQSLCCHATNDLEGALELLRSALSLAEPEGYVRTFVDYGLPMEQLLQLTAVRGIAA
                     D"
BASE COUNT          634 a          634 c          605 g          653 t
ORIGIN      
        1 tattgttagt gagatgcgaa ttagtgtctt aacgccctat tcttcattga acttcatact
       61 ctatactttc tcgacgcctt agatttgtca ggcctgacgg cgactacgac gtcatctatt
      121 gccatcagaa tgaaagacag tttactgctg acgaaactct atatcccgtc actaagtccc
      181 agggttgtgc accgtccaca ccttatctcg cgtttgaacg aaggtttagc gaggggctgc
      241 aaactcaccc tgctatccgc gccagtggga tatggcaaga catctctaat cagtgattgg
      301 ctatctaccc taaacagccg agctgcctgg ctttcattgg acacggaaga tgacgaccct
      361 ctgcggtttt gggtatacgt gattgccgcg ctgcaaacca tccaacctga actcggccag
      421 acggcgctta ctgccctcca aacggcacaa atgcctccga tcgaaaatat gctcattgat
      481 ctgattaacc agatttctac gctctcagac aaaattatcc tggttctcga tgatacccat
      541 ctcattgaaa caaccgaaat acaccagagc ctcaattttt tacttgatca tttgccaccc
      601 caaatacatc tggtcttcat aacccgagaa gatcctccat taccgctgcc tcagatgcgt
      661 gcgaagggac aaattagcga gctgcgaatc aaggatttac gcttcacgat ggaggagagc
      721 gctgaatttt taaatcacaa aatgggactt aatttgcacc ccaaaggcat taccgccttg
      781 ggacaacgca cagaaggttg ggccgccgga ttgcaaatgg ctgcgctttc actgcacgag
      841 ttggatgatc ctgctgcatt cattgaagtt tttgcgggtg acaatcgtta tgtggccgat
      901 tacctgatct cagaagtatt ggatcgccaa ccagatcaca tccgccattt ccttctgcaa
      961 accgcaatcg ttgaccggtt cacatcatct ttgtgcgatg cattgatcgg ggacacatcc
     1021 caccaaagca gcaggatcat cgaaaaaatc gaggcgctag gccttttcat catgcccctt
     1081 gatcatgttc gccagtggta tcgctatcac cagttatttg cggacctact tcgttatcgt
     1141 cttcgacaag acgatccatc cagattcatg gatttaaatc gaaccgccag tcgctggtat
     1201 gaacagcaag gcctaatcga tgaagcggtt aagtatggat tggtcggtga agatcacgag
     1261 tatgtagcgg aactgatcga gatctctggc ttagccatgt ttggacgaaa ccagttcgct
     1321 agactgcaaa attggatcag cgccctgcct gaaaagatta ttcagaaaca tccgtattta
     1381 tctgttcttc tggtctgggt cggctcattg accggacaga gcgatcttgc caggagacat
     1441 ctggccttgg ctgagaagaa tctttcttcg gcacctgccg atcagcatgc tgaaattatt
     1501 agccatgtcg ctctgcttcg ggcatatgcc acgcgcacca gtggcgacct ggactcatcc
     1561 atcaaacatg cacaggaagc cctgagttac ctcccggaga ataatgtttt cttagactgt
     1621 accattcacc taaacctggg aggtaactac tggctcaaag gaaatttccc agcggttgag
     1681 gggccgctca aacatgccat ttctttcata gatatccccg aggtcgaata tcctgttctt
     1741 gcagcggctg gatttctcgc gaatgcctac ctgcagcagg ggaaactgca taacgcggag
     1801 gccttgtgta aaaaaattgt tgagcgcaat agctaccatg cccacccggc tgctgcctat
     1861 gtgttacttg agctaggcga gttgttcttc gaaagaaatg atctcgacgg agctttggag
     1921 gttctctcaa agacaattca gatcggagaa atcgcggata aggttgttaa tgttattcgc
     1981 gcccgccagc ttttagcacg agtctatcaa gcgcttggca accaggaaga ggtggccaac
     2041 ttgatgatac aggctgacga gctgtttaaa caaaccagcc cccgctatca ggtcatgcat
     2101 caaatagaat atgagtatta tcgcgtccgc tgtctgctct ttcagcaaag tacgtggggt
     2161 gctctacaat gggcagatga ttatgcaaaa aggcgcgagc cggtcaacaa tccgtgggct
     2221 ttgttgaatg aactcgtcta cgctcacgtt ttgcttattg ataggcgacc tgatcaggcg
     2281 ctttcgatct taaaaccatg cgaagactct gccagatcat atggtgcaag gggatgggtt
     2341 gttcaaaatc tggcgcttca gtctctctgc tgtcatgcga cgaatgattt ggaaggggca
     2401 ttggaattgc tgcgcagtgc gctcagccta gctgagccag agggctatgt ccgcaccttt
     2461 gtcgattacg gattgccaat ggagcagttg ttgcaactta ctgcggtgcg cggaatagca
     2521 gcggat
//
LOCUS       DBNHOH010000444         2372 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1243626, whole genome shotgun sequence.
ACCESSION   DBNHOH010000444 DBNHOH010000000
VERSION     DBNHOH010000444.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1243626"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..809)
                     /gene="rnc"
                     /locus_tag="AC16V7_07810"
     CDS             complement(81..809)
                     /gene="rnc"
                     /locus_tag="AC16V7_07810"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIR6257063.1"
                     /translation="METAQGSLRGESPQDLIQRLGLSITDFGLIARALTHRSFANEHN
                     DVLEDNERLEFLGDAVLDFLVGAWLYDHYPELKEGDLTRLRSGLVRTEQLAEFAREID
                     LGRAMRLGRGEDDGGGRERIALLCGTFEALIGALYLSDGLPLVQQFVEPMLIPAAERI
                     LVSLEEQDPKSLLQEWIQAQGYDHPTYRTVTANGPDHAKIFEVEVIFNGEIQGRGLGH
                     SKQAAAKSAAREALTNLGIIRNDD"
     gene            complement(973..2211)
                     /gene="fabF"
                     /locus_tag="AC16V7_07815"
     CDS             complement(973..2211)
                     /gene="fabF"
                     /locus_tag="AC16V7_07815"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIR6257064.1"
                     /translation="MAERIVVTGMGAISPLGHSANQSWENAIHGVSGVGPITQFDTSD
                     YLVKIACEVKDFRPEDYMEAREARRRDRFTQLAAVAAQEAMRQSGLDVKAEKPERIGL
                     IISSAIGGLKTLQDSIFTLKEDGPRRLSPFVIPMLMSNGAAGMMAIEYGMTGPSFSVA
                     SACASGADGIGTAWMMLRSGMIDVALTGACESTITPIGIGAFDRLGAMSRRNDDFSMT
                     PQPFDKNRDGLVMGEGAAVLVLESEAHARARGAEILAELAGYAATADAFHITAPAEDG
                     AGGALAMKLALNASGVNPEDVDYINAHGTATPLNDISETRAIKSAFGKLAYNIPVSST
                     KSMTGHMMGATGALEATFCVQAVRNNVIPPTIHYQTPDPECDLDYVPNQAREMTVNVA
                     LSNAFGFGGHNAVLVIKKYR"
BASE COUNT          591 a          664 c          545 g          572 t
ORIGIN      
        1 caaaaactgt ctggctagca aaggttttat aaccttgcag ctcgagggat ttcagacgca
       61 tcaggagtgc tgttttatct tcagtcatca ttccgaatta ttccaagatt ggtaagcgct
      121 tcgcgagcag cggatttcgc agcggcttgt ttgctatgac ccaaaccgcg cccctgaatt
      181 tcgccattaa aaatgacctc cacttcgaaa attttagcat gatccggtcc attggcggta
      241 acggtgcgat aggtggggtg atcataccct tgagcttgaa tccactcttg aagtaagctc
      301 ttagggtctt gctcttcgag ggagacaagg atcctttcag cggcgggtat cagcatgggt
      361 tccacaaatt gttgaacaag gggaagacca tcgctcagat atagtgcccc aatcagcgcc
      421 tcaaacgtac cgcacagtaa tgcaattcgt tcacggcctc cgccatcatc ttcaccgcgt
      481 cccaggcgca tcgccctacc caaatcaatt tcgcgggcaa attcagctaa ttgttcagtg
      541 cgcaccaatc ccgaacgcaa tcgtgttaaa tcgccttcct ttaactcagg gtaatgatca
      601 tatagccagg cgcctactaa aaaatcgagg accgcatcgc ccaaaaattc taggcgctca
      661 ttgtcctcca gtacatcgtt atgttcgttg gcaaatgagc ggtgcgtgag cgcacgcgca
      721 atcaaaccga aatcggtgat tgacaacccc aaacgttgga ttaaatcctg gggggattca
      781 ccccgtaggg atccctgagc agtttccatg tataacctcc cggaactcag ggagcgtcag
      841 acgccccacg ccgggtcgag cgtctggcgt tccattcgtt ccttctttct acctattgca
      901 gatttttgac gcgtggcagg ttgaccctca caattgtact tccaaccaac cacatctcac
      961 ataaagaccc tctcatcgat actttttgat caccaacact gcgttgtgac cgccaaaacc
     1021 aaaggcatta cttagagcta cattaacagt catctcccga gcttggttag gaacataatc
     1081 cagatcgcat tcgggatcag gagtttgata atggatcgtt ggagggatga cattgttgcg
     1141 cacagcttgt acacaaaacg tggcttccag cgcgccagta gcacccatca tgtgtccggt
     1201 cattgatttc gttgacgaaa cagggatatt atacgccaat ttaccaaacg cggacttaat
     1261 cgcgcgggtc tcagagatat catttaatgg tgttgcagta ccgtgggcat tgatgtaatc
     1321 cacatcttcg ggatttactc cacttgcatt caaggcaagc ttcatcgcca gtgcaccacc
     1381 tgcgccatcc tctgctggag cggtgatatg aaaggcgtcg gctgtagccg cataacctgc
     1441 caactcagcc aatatctccg ctcctcgagc gcgggcatga gcctcggatt ccaataccag
     1501 gacagccgca ccctcaccca tcactagacc atcacggttt ttatcaaagg gttgtggtgt
     1561 catagaaaag tcgtcatttc ggcgtgacat ggcacctaaa cggtcaaatg cccctattcc
     1621 gatgggcgtg atagtggact cgcaggcgcc cgttagtgca acatcaatca tgccagaacg
     1681 cagcatcatc catgcagtac cgatcccatc tgcaccagag gcgcaggcag aagctacaga
     1741 gaacgacgga ccagtcatac cgtattcaat cgccatcatg cctgccgcgc catttgacat
     1801 caacatcggt ataacaaacg ggctcaggcg gcgggggcca tcctctttta atgtaaatat
     1861 cgaatcctga agagttttca agccgccgat cgccgaggat atgatcaacc cgatgcgctc
     1921 aggtttttcc gccttaacgt ccagcccaga ttggcgcatt gcttcctgag cagctacggc
     1981 agccaattgt gtgaagcgat cgcgacgacg cgcttcacgt gcctccatat aatcctcagg
     2041 acgaaagtct ttgacctcgc atgcgatctt gaccaaataa tcgctggtat caaattgggt
     2101 gattggtccg acacccgaga cgccatggat agcgttctcc catgactgat tcgccgaatg
     2161 acctaacggg ctgattgctc ccatccccgt gacgacaatg cgctctgcca ttatgatgtc
     2221 tccttagttt gttgctgttc ctatccactt aaatcctgat ccggtcaaaa gtacaaccac
     2281 ggggtcaggc atccgacctg ccaattgttc caacgcgctc caaattaccg ctgaggtggg
     2341 ctccacataa aaacctaaat gagccaatgc ct
//
LOCUS       DBNHOH010000445         2185 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_602567, whole genome shotgun sequence.
ACCESSION   DBNHOH010000445 DBNHOH010000000
VERSION     DBNHOH010000445.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_602567"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..1060
                     /locus_tag="AC16V7_07820"
     CDS             257..1060
                     /locus_tag="AC16V7_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6257065.1"
                     /translation="MVSVSESAPVVEMRNIKKNFAAVQALRGVDLVLHHNEVLGLLGD
                     NAAGKSTLMKVLSGAYIPDEGEIILDGEKVQITDPSHARRLGIEMVYQDLALANNLNV
                     AANVFLGRELVGSQFGPVGVMDSHHMEVETQRLLDRLKIDIPSVRLLVERLSGGQRQA
                     VAIARATAFNSKVTIMDEPTAALSVAAISKVLDLVRELKAQGASIIIISHRLEDIYQV
                     SDRMIVLRHGRKVGDCPVEGDIDEFRERVVAYIVGARDDFANKEVGSVK"
     gene            1081..>2185
                     /locus_tag="AC16V7_07825"
     CDS             1081..>2185
                     /locus_tag="AC16V7_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019414470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HIR6257066.1"
                     /translation="MSYFKDLQKLFQQNIREYGMYIAFFVIIVFFSFTTKGLFVSSRN
                     IGNLVNATSYIAVLAVGMTLVIVIRHIDLSVGFLAGFLGAIAAISMKFWLNPSYLTIP
                     LILGLGILAGLLTGFLVAQMKIPAFVATLAGSLGYRGALQLVTSSTGTIIISDDMFNA
                     IGNGFIPDIPGLEILPEIHKLTLLLGLGAIILFIIGEINSRRKTLSYDFEVLSMSMFI
                     TKLVFVSAVIGGITWVLAGYQGLSWAAVVLLAVVMIYSFITTKTVLGRHIYAVGGNPE
                     AAELSGISVKRITYLVFGSMGMLAALSGILYASWLRSATTIAGTLFELDAIAAAFVGG
                     VAVAGGVGKVTGSIIGALVMASLTNGMNLMGVGI"
BASE COUNT          510 a          495 c          584 g          596 t
ORIGIN      
        1 ttgtggaagc cgtgattgac tccggttact gggatgccag cgaattcact ggattaccgt
       61 aagatctaac agttcatgaa cgtaggaata gtgatgggta aaaccatcac tattcctata
      121 tttcgtatat tactcaatct ttgacaaaca catttgtaaa acaatcgctt ttgaattaaa
      181 ctatctatta agcagaaaaa gaggcgtcat cgtctaagat cgattcttta taaatacaat
      241 tctcaaggag aaagcgatgg tatctgtgtc agaatccgcg cctgttgtgg aaatgcgcaa
      301 tatcaaaaag aactttgctg ctgtccaggc gttgcgcggg gtagaccttg tattgcacca
      361 taatgaagta ttgggtttgc taggcgataa cgcggctggc aaatctacat tgatgaaggt
      421 tctcagtggg gcatacatcc ctgatgaagg agagatcatc cttgatggcg agaaggtcca
      481 aatcactgac ccttcgcatg cgcgccggct gggcatcgag atggtctatc aggacctggc
      541 tctagccaac aaccttaacg tggctgccaa tgttttcctg ggacgggagc tagtaggttc
      601 ccaatttggc ccggttggtg taatggacag ccatcacatg gaggtggaga cccaacgcct
      661 cctggatcgg cttaaaatcg atatcccatc ggtaagattg ttggtagaga gactgtccgg
      721 gggacagcgt caggcggttg ccatcgcccg tgcaacggct ttcaacagca aggtcaccat
      781 tatggacgag ccaacggcgg ccttgagcgt ggcagccatc agcaaggtgc ttgatctggt
      841 gcgtgagctc aaggcgcagg gcgcatcgat tatcatcatc agccaccggt tggaagacat
      901 ataccaggtg agcgaccgga tgatcgtgct gcgccacggg cgcaaggtag gcgactgtcc
      961 agttgaaggc gacattgatg agttccgtga gcgcgtggtg gcttacatcg ttggcgcccg
     1021 ggatgatttc gcaaataaag aagttggctc ggttaaataa agcaggtggg agcgctcaag
     1081 atgtcttatt tcaaggatct ccaaaaatta ttccagcaga atattcgcga atacggcatg
     1141 tatatcgcat ttttcgtgat catcgtattc tttagcttta ccacaaaagg gcttttcgtt
     1201 tcgtcaagaa acatcggcaa cttggtaaat gcgaccagtt acatcgcagt tctggcggtt
     1261 ggtatgacgt tggtcatcgt catccggcat attgatcttt cggttggttt tcttgcgggc
     1321 tttctcggtg caattgcggc gatttccatg aaattctggc tcaatccctc ctacctgact
     1381 attccgctta tcctggggct tggtattcta gccgggctgc tcaccggttt cctggttgcg
     1441 cagatgaaga taccagcctt tgttgcaacg ctagccgggt cgctgggtta ccggggcgcg
     1501 ctgcagttag taacctcgag tacgggaacg attatcatct cagatgatat gtttaacgct
     1561 ataggcaatg gttttattcc cgacattccc ggccttgaga tattaccgga aatacacaaa
     1621 ttgacccttc tcctcggatt aggagccatt atcttattca tcataggcga aatcaacagc
     1681 cgcagaaaga cgctatctta tgactttgaa gttctttcga tgagcatgtt catcaccaag
     1741 ttagtattcg tgtcagcggt gatcggaggt atcacctggg tcctcgctgg ataccagggt
     1801 ctctcctggg ctgcggttgt cttacttgct gtagtaatga tttattcttt catcactact
     1861 aagacggtgc tcggcaggca tatctacgcc gttggcggaa atccagaggc tgcagagcta
     1921 agcggtatca gtgttaagag gattacctat ttggtttttg gttcgatggg aatgcttgcc
     1981 gccttgtctg gtatccttta tgcatcctgg ctgcgttctg ccaccacaat cgctggaacc
     2041 ctgtttgaat tggatgccat cgctgcggct ttcgttggag gcgtagcggt ggcgggtggc
     2101 gtcggcaagg tcactggatc gatcattggt gctctggtta tggcgtcatt gacaaacgga
     2161 atgaacctga tgggcgtcgg catat
//
LOCUS       DBNHOH010000446         2087 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1307442, whole genome shotgun sequence.
ACCESSION   DBNHOH010000446 DBNHOH010000000
VERSION     DBNHOH010000446.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1307442"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="AC16V7_07830"
     CDS             complement(<1..307)
                     /locus_tag="AC16V7_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M20"
                     /protein_id="HIR6257067.1"
                     /translation="MLAQALDYARSHQGKSLDRLCEWLRIPSISTLAENAQDVRRAAQ
                     WTSEALREMGLDHIEVVETSGHPLVYADWMGADGPTLLIYGHYDVQPVDPLDEWRTPP
                     "
     gene            complement(407..1744)
                     /locus_tag="AC16V7_07835"
     CDS             complement(407..1744)
                     /locus_tag="AC16V7_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6257068.1"
                     /translation="MKRTLFHISYSMGNFANTIAYQVFGNRIQFYYVDILGLNAALAG
                     ILWTIYGVWNAINDPLMGQLSDRTRTRMGRRVPYVFFGAIPLGLSFFFLWTPPTQSPW
                     MLAAYFFALLFIFDTIYSLTMIAYNALFPEVAPTLRDRVDLSATREILATVALLLAFI
                     LAPILAEEVGYIWMGAIMGIFVAIGYLVSMIGVRENLANIKDDSVGIIPSLKIVLSSV
                     PFRWFVGANMAKEYVWLALAAMLPFWRKYALGIQGEGLVFGIRMGAGDQEAILLGLPI
                     VLCIPCLLIWRPIVPRLGNRKTWILASFVFIPGFLVVAFANNFYTGLLGTVLAAPGLA
                     GSMIMPFPVISEVIDDDASRHGYQRSGIFFGMSWGIVKLAFSAQGVLFASVMSAAGYA
                     AGSVVQTASAVSGIRFLIGITPIIACLIVAYCMYRYPLGAAQIKDAHKAEELT"
BASE COUNT          476 a          596 c          582 g          433 t
ORIGIN      
        1 agggcggcgt gcgccactcg tccaacgggt caacaggttg cacgtcatag tgcccgtaga
       61 tgagcagggt agggccatcc gcgcccatcc agtcggcgta gacgaggggg tgcccgctgg
      121 tctccacaac ttcgatgtga tcgagtccca tctcgcgcag ggcttcgcta gtccattggg
      181 cggcgcggcg cacatcctgt gcgttttctg ccagcgtgct gatacttggg atgcgcagcc
      241 attcgcatag cctatctaga gattttccct gatggctgcg ggcgtagtcc agagcttgtg
      301 ctaacatgat ttatccttca gccaggcgag ttttgttgtt ggattcatct catctcctat
      361 ttgacctcga tccttgacct cccgcaggtt ctgaacctgc gggaggttaa gtaagctctt
      421 cagccttgtg agcgtctttg atctgggctg ctcccaaggg gtacctgtac atgcaatagg
      481 ctacgatcag gcaggcaatg atcggggtga tgccgatcaa aaaacggatg cctgatacgg
      541 cgctggctgt ttgcaccaca cttccggcgg catatcctgc agccgacatc accgacgcaa
      601 agagcactcc ctgggcggaa aaggccagct tgacgatgcc ccagctcatc ccaaagaaaa
      661 tgcccgaccg ctgatagccg tgacgggatg cgtcatcgtc gatcacctcc gatataaccg
      721 ggaagggcat aatcatcgag cctgccagac cgggagccgc cagtaccgtt cctaataaac
      781 cggtgtagaa attattggca aaggcgacca caagaaaccc cgggataaag acaaagctgg
      841 caagtatcca ggttttgcgg ttgcccaggc ggggcacgat cggacgccag attaacaggc
      901 aggggatgca cagcacaatc ggcagcccca gcaagatggc ttcctggtcg ccagcgccca
      961 tgcgaatgcc gaataccagc ccctcaccct ggatgcccag ggcgtacttg cgccagaagg
     1021 gtaacatagc tgccagcgcc agccagacgt attcctttgc catattagcg cccacgaacc
     1081 agcggaaagg cacgctggat agcacgatct tcagggatgg gataatccca accgagtcat
     1141 ccttgatgtt ggcgaggttt tctctcaccc cgatcatgga gactaagtag ccaatcgcga
     1201 cgaagatgcc catgatggcg cccatccaga tgtagcccac ctcttccgct aggatgggcg
     1261 ccaggatgaa ggctagcagt aaggcgacag tcgccagaat ctcgcgcgtc gccgacaggt
     1321 ccacccgatc tctcagcgtg ggagccacct cggggaataa ggcattgtat gcgatcatgg
     1381 ttaggctgta gatggtatcg aagatgaata gcagggcgaa aaagtatgcc gccagcatcc
     1441 acgggctttg ggtgggcggc gtccacagga agaaaaatga cagaccgaga ggtatggcac
     1501 cgaagaagac gtatggcacg cgccgcccca tgcgggtgcg cgtccggtcg gagagctgcc
     1561 ccatcagcgg gtcgttgatg gcattccaca cgccatagat ggtccacagg atgcctgcca
     1621 atgctgcatt caggcccagt atatccacat agtagaactg gatgcggttg ccaaacacct
     1681 ggtaggcaat agtgttggcg aagttcccca tggagtaact gatgtggaaa agcgtcctct
     1741 tcatgatgcg cttgcctccc ggctttggtc ttggtggatc cgccttgctg ccccacccac
     1801 atcgtaccca ttataaaaag ttaaacccct ttgccctata tgtggttacg gtattgacaa
     1861 tcttaacgcc aagtgtagct cgttgaagcg cgcataaaac tcgcccgcgc tatccgtctc
     1921 caatcgaggg cggcagcctg aaaacccccc ggtgatctac gccgttgtgc aacgggtgat
     1981 aggtactact cgaatgtcaa gtacgttaga actaaacggt ttgcttttct actggcgcca
     2041 ggttttcctt tccgtacacc ttgaccaccc gcactgccca gaagacg
//
LOCUS       DBNHOH010000447         3151 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_731093, whole genome shotgun sequence.
ACCESSION   DBNHOH010000447 DBNHOH010000000
VERSION     DBNHOH010000447.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_731093"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="AC16V7_07840"
     CDS             <1..620
                     /locus_tag="AC16V7_07840"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6257069.1"
                     /translation="ISIIALKEARQRIGEHGHYVVCDIANLPFKAEAFEGIVSLHTIH
                     HLPETEHLQAYSELYRLLSPGCAATVVNGWPGSRLMRWAEPLIHLNRRIRSILRRLLK
                     RTQPTAHRARHSARDTSQVTADTPKGTYTSRHDAAWIRSEVARHMPVQIWVWRSVSVR
                     FLRNFIYPQLGGRLWLRLLYWLEERFPHWLGENGQYPLIVIRKVE"
     gene            719..1732
                     /gene="pseB"
                     /locus_tag="AC16V7_07845"
     CDS             719..1732
                     /gene="pseB"
                     /locus_tag="AC16V7_07845"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HIR6257070.1"
                     /translation="MDWSDKVVLVTGGTGSFGKKFIRIMLDEYHPAKLIVFSRDELKQ
                     HEMRVSGFDHPSLRYFIGDVRDVERLRRAMHGVDIVIHAAALKQVPACEYNPLEAIKT
                     NILGSSNVIEAALDMTVKKVMALSTDKAVNPVNLYGTTKLAAEKLFVQSNAYAAGTST
                     RFSCVRYGNVVGSRGSVVPIFVQQRKNGKLTITDERMTRFWLTLEQGVRFVIRCTEQM
                     LGGEVFVPKIPSMKIIDLAQAIAPDTEMEIIGIRPGEKLHEVLIHEDEARSAVELDDM
                     FIVQPATELWFGHEWQKLGKPLPDGYRYASNTNPEWLDIQQIQQMIAPFERDEGQIMI
                     DEG"
     gene            1845..2777
                     /locus_tag="AC16V7_07850"
     CDS             1845..2777
                     /locus_tag="AC16V7_07850"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009226 - nucleotide-sugar biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6257071.1"
                     /translation="MRILVTGASGLLGLNLALSAAGQAGEGHLVIGTVKDHALKTDAF
                     RTIQTDLLIPGAVERLLDQTQPDWVIHCAAMANVDACEADPEQAKQMNSELPGKLAAY
                     VARGGARLVHISTDAVFDGQRGDYLEEDVPNPLSIYARTKLEGELSVAQANPEAIIAR
                     VNLFGWSISGARSLGEFFFYNLSAGKRVRGFTDVFFCPILANEIAPILIRMLEKKLSG
                     LYHVVSRECTTKYEFGVRIAHRFGLDESLITPSSVADAGLQAVRSPKLTLRTDKLAAA
                     LGEPPPDLSTGIEGFYTLYQQGYPQILKGIGGRG"
     gene            2858..>3151
                     /locus_tag="AC16V7_07855"
     CDS             2858..>3151
                     /locus_tag="AC16V7_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HIR6257072.1"
                     /translation="MNLLLEIARCPNVITCVKKPSEQHPCSQVIASQKATSLDSFHVP
                     EPWNGLIEQAPLLFISSNPSFNRKEEFPLWSWDESLIEDYFTHRFGGGKKLWVK"
BASE COUNT          749 a          862 c          877 g          663 t
ORIGIN      
        1 atatttcaat catcgccctg aaggaagccc ggcagcgcat cggggagcac gggcactacg
       61 tggtttgcga tatagccaac ttgcccttta aggcggaggc attcgaaggc atcgtctcgc
      121 tgcacacgat ccatcaccta ccagaaactg aacatttgca agcctattcg gagttatacc
      181 gcctcctgtc ccccgggtgc gcggcaacgg tggtcaatgg ctggcctggc agccggctga
      241 tgcgctgggc tgagccgctc atccacctaa accgccggat aaggagcatc cttcgccgcc
      301 tgctaaaacg gactcagccc accgcacatc gcgctcggca ctccgcacgt gacacttcgc
      361 aagtgacagc tgacaccccc aaaggaacat ataccagccg tcatgacgca gcctggatca
      421 ggagcgaagt cgccaggcac atgccggttc aaatctgggt ctggcgcagt gtgagcgtgc
      481 gcttcctgcg caattttatc tacccccagt tgggcggacg cctgtggctg aggctgcttt
      541 actggctgga ggagcggttc ccgcactggc ttggcgaaaa cgggcagtat ccgttgattg
      601 tcataagaaa ggtagaatga agaatttcgc cgtagagatg caatatatac tgagataccg
      661 caggagaaac atctgcatac tcagtgtctc tacggtaagt tagcataagc gaggaattat
      721 ggattggagc gataaagttg ttctggtaac cggcggaacc ggatcgtttg gtaaaaagtt
      781 catcaggatc atgctggacg agtaccatcc cgccaagctg atcgtcttca gccgcgacga
      841 gctcaagcaa cacgagatgc gcgtgagcgg ctttgatcac cccagcctgc gctatttcat
      901 cggcgatgtg cgcgacgtag agcgcttgcg ccgcgccatg catggggtgg acatcgtcat
      961 ccacgctgcg gccctcaagc aggtgccagc ctgcgagtac aacccgctgg aggcgatcaa
     1021 gaccaacatc ctgggcagca gcaacgtcat cgaggcggct ctggatatga ctgtaaagaa
     1081 ggtcatggca ctgagcacag ataaggccgt caatccggtt aacctgtacg gcactaccaa
     1141 gctggctgca gagaagctct ttgtacagag caacgcctac gccgccggca ccagcacccg
     1201 tttcagctgc gtgcgctatg gcaacgtggt gggcagccgg ggaagcgtcg tcccgatctt
     1261 tgtgcagcag cgtaagaatg gcaagttgac catcaccgat gaacgcatga cccgcttctg
     1321 gctcaccctg gaacagggcg tgcgctttgt catccgttgc accgagcaaa tgcttggggg
     1381 cgaagtcttt gtgcccaaaa tacccagcat gaagattatc gatctggccc aggccatcgc
     1441 tccagacacc gagatggaga tcatcggcat ccgcccgggt gagaagctcc acgaagtgct
     1501 cattcacgag gatgaagccc gctctgcggt ggagctagac gatatgttta ttgtgcagcc
     1561 tgctaccgag ctgtggttcg gtcacgagtg gcagaaactt ggcaagcccc tgccggatgg
     1621 ctatcgttac gccagcaaca ctaatccaga gtggcttgac atccagcaga tccaacagat
     1681 gattgcaccc ttcgaaagag acgaagggca aataatgatt gacgaaggat gaaagctgaa
     1741 gcattaaatc tggggctgat gaatattggt cgacctcaga taatgagtag acggaggacg
     1801 aaagacgcag aacaatagct aattgctgct tatataggtg aattatgcgc attctggtca
     1861 ccggagccag cggcctgcta gggctgaatc tggctctgag cgcggcgggg caggccggag
     1921 aaggccacct cgtcatcggc acggtcaagg atcacgctct caagaccgac gccttccgta
     1981 ccatccagac cgatttgctc atccccggcg cagtggagcg cctgctcgac cagactcaac
     2041 ccgattgggt gatccactgt gctgcgatgg caaacgtaga cgcctgcgaa gctgatcctg
     2101 agcaggcaaa acagatgaat agtgagctcc ctggaaaact ggcagcctat gtcgccaggg
     2161 gcggagcgcg cctggtgcat atctctaccg atgccgtctt tgacgggcag cgcggcgatt
     2221 accttgaaga agatgtcccc aatccattga gcatctatgc ccgcaccaag ctggagggtg
     2281 agctcagcgt agcgcaggca aatcccgagg cgatcatcgc ccgcgtcaac ctgttcggct
     2341 ggagcatcag cggggcccgc agcctgggcg agttcttctt ctataacctc agtgcaggca
     2401 agcgcgtgcg cggcttcacc gacgtgtttt tctgccccat cctggcaaac gagattgccc
     2461 caatcttgat ccgcatgttg gagaagaagc tcagcgggct gtaccatgta gtcagccggg
     2521 aatgcaccac caaatatgaa ttcggcgtgc gcatcgccca ccgcttcggc ctggatgaaa
     2581 gcctgatcac cccctcttcg gtggcagacg ctggcttgca agccgtgcgc tctcccaagc
     2641 ttaccctgcg caccgataag ctggctgctg ccctgggtga gccgccaccg gatttatcca
     2701 ccggaatcga gggcttttac acactgtatc aacagggtta tccacagatt cttaagggga
     2761 tagggggaag gggataggga aaaactcccc ttacatcctg aacaatcaat taaagtattg
     2821 cttcatggga tttcttgtgc atattttgag gtgaaagatg aatctgttac tagaaatcgc
     2881 tcggtgtcca aatgttatta cttgtgtcaa gaaaccatcg gagcaacacc cttgctccca
     2941 agttattgcg agtcaaaagg ctaccagcct agatagtttt catgttcccg aaccctggaa
     3001 tggcttgatt gagcaagccc ctttgctgtt tattagttcc aatccttcat tcaacaggaa
     3061 agaggaattt cccctttggt cgtgggatga aagcttaatc gaggattatt ttacccatcg
     3121 tttcggaggc ggaaagaagt tatgggtaaa a
//
LOCUS       DBNHOH010000448         1976 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_794596, whole genome shotgun sequence.
ACCESSION   DBNHOH010000448 DBNHOH010000000
VERSION     DBNHOH010000448.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_794596"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="AC16V7_07860"
     CDS             <1..462
                     /locus_tag="AC16V7_07860"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253450.1"
                     /note="catalyzes a two-step reaction, first charging a
                     glutamine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HIR6257073.1"
                     /translation="VRLRYAYFIRCEYAVKDENGEVVELHCTYDPATRGGDAPDGRKV
                     KSTIHWVSASHALQVEARLYDRLFTKENPDDAPEDGDFTDNLNPESLVNLPDSYVEPE
                     LRGTLVGSIYQFERQGYFCVDPDSTKDKLVFNRTVSLRDTWARIEKKLQSG"
     gene            complement(559..>1976)
                     /gene="gltX"
                     /locus_tag="AC16V7_07865"
     CDS             complement(559..>1976)
                     /gene="gltX"
                     /locus_tag="AC16V7_07865"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR6257074.1"
                     /translation="TALYNYLIARQTGGQFLLRIEDTDRKRYVPGAEEELIDSLHWLG
                     LQWDEGPDVGGPYAPYRQSKRKEIYQHHARKLVEMGHAYYCFCTPQHLAQVRQEQQKL
                     KQSTLYDGLCRKLDPDEADKRVANGESHVVRFKTPKEGSITAVDRLRGPITVENHTLD
                     DYILLKSDGLALYHLAAMVDDHLMKITHVLRSSEWLPTFPLHAHIIRAFGWQEPEWVH
                     LSVFLKPSGKGKMSKRESADLKQSGYSIFIKDLEELGYLPEAVVNWIALMGWSYDDHT
                     EFFTLPDLVEKFSLDKLNPSPAAINFTKFDHFNGLHIRHLAVNDLAQRVKPFFQAAGY
                     SVDDDRLLKVVPLIQERIITLEDAPAMGGFFFRDDLQPAPDDLVGKKMTPGDSARAAR
                     EAYQILLPLGEINIETAEEPLRALAESLEISAGQLFGILRAAVTGQTVSPPLFESMAV
                     IGKTKVLERIQNAINALEKME"
BASE COUNT          454 a          496 c          545 g          481 t
ORIGIN      
        1 gtgcggctgc gttacgcgta tttcatccgt tgcgaatacg cagtaaaaga cgagaatggg
       61 gaagtggtcg agctgcactg cacttacgat ccagccacgc gcggtgggga tgcccccgat
      121 gggcgcaaag tcaaatcaac catccattgg gtgtccgcat ctcatgcctt gcaagtggag
      181 gcgcgcctgt acgaccggct gtttaccaag gaaaaccccg acgatgcgcc tgaagacgga
      241 gattttaccg ataacttgaa cccggaatct ttggtgaacc tgccggatag ctacgtcgag
      301 ccagagctga gaggtacgct ggtgggcagc atttaccagt tcgagaggca agggtatttc
      361 tgcgtggatc cagactcaac gaaagacaaa ctggtgttca atcgaactgt atctctgaga
      421 gatacttggg ccaggatcga aaaaaaattg cagtctggat agctttttat tatttcaaac
      481 caaagacgaa aaaacctttt ccaccgaggt gtaggttaat tgggcagacg gaactcccca
      541 aacctcacct cggtgttctt actccatttt ttctaaggca ttgatcgcat tttgaatgcg
      601 ttccagcact ttggttttgc caatcaccgc catgctctca aaaaggggcg ggctgacggt
      661 ttgtccggtg accgcggcgc gcagtattcc aaaaagctgc ccggcactga tctccagcga
      721 ctcagccagg gcgcgcaagg gctcttcagc ggtctcgata ttgatttccc caaggggtag
      781 gaggatctga taagcttctc gggcggctct tgccgaatcg ccgggggtca tcttcttgcc
      841 aactaaatcg tctggagcag gctgcaggtc atctctgaaa aagaatccac ccatcgcggg
      901 tgcatcttcc agggtgatga tgcgctcctg gatcaatggg acgactttta aaagcctgtc
      961 gtcatccacg ctgtatcctg cagcttgaaa gaatggcttc actcgttgtg ccaggtcatt
     1021 cactgccaag tgacggatgt gcaatccgtt gaaatggtcg aacttggtga agttgatcgc
     1081 ggctggggag ggattaagct tatccaggct gaatttttcc accaggtccg gcagtgtaaa
     1141 gaactccgta tggtcatcgt agctccaacc catgagagcg atccagttaa ccacggcttc
     1201 tggcaggtag cccaattctt caagatcttt gataaagata gaatagccgc tctgtttcaa
     1261 atcggcggat tcacgcttgc tcatttttcc cttgccgctg ggtttaagaa atactgaaag
     1321 atgcacccat tcgggttcct gccaaccgaa agcgcggatg atatgagcgt gcaatgggaa
     1381 ggttggcagc cattcagagc tgcggagaac atgggtgatt ttcatcaaat ggtcgtcgac
     1441 catagcagcc aggtgataga gcgccaaccc gtcagatttg agcaggatgt aatcatccag
     1501 ggtgtggttt tctaccgtaa tagggccgcg caatcggtct accgcggtga tgctgccttc
     1561 ttttggggtt ttgaagcgta ccacatgaga ctcgccgttg gcgacacgct tgtcggcctc
     1621 atcagggtcc agcttgcggc ataagccatc atataaggtg ctttgcttta gtttttgctg
     1681 ctcctggcgc acctgggcga ggtgttgggg ggtacaaaag cagtaatagg catggcccat
     1741 ctcgactaac ttgcgggcat ggtgctggta tatctcctta cgtttggact ggcgatatgg
     1801 agcataagga ccacccacgt cggggccttc gtcccattgc aatcccagcc agtgcaggct
     1861 atcaatgagt tcctcctcag cgccgggaac gtatcgcttg cggtcggtat cttcgatgcg
     1921 tagcaagaac tgtccaccgg tctggcgggc aatcagatag ttatacagcg cggtgc
//
LOCUS       DBNHOH010000449         2060 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1692697, whole genome shotgun sequence.
ACCESSION   DBNHOH010000449 DBNHOH010000000
VERSION     DBNHOH010000449.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1692697"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1495)
                     /locus_tag="AC16V7_07870"
     CDS             complement(<1..1495)
                     /locus_tag="AC16V7_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HIR6257075.1"
                     /translation="MTALTTLAEEESRAETEDRINNFSITVATVNGSGSQTSNLTILR
                     ALFKMGIPTSGKNLFPSNIQGLPTWYTIRVSKDGFLARREEHEIVVAMNPATFSRDLA
                     DVSPGGVFFYADDIKMSVNRDDIVSYAMPVKQIVRESGAPSSLRDYVANMVYVGILAQ
                     MLGIDLDKIYQAISFHFKGKTKPIEMNWEVVQASANWAAQNLEKTDPFGVEPMDATTD
                     YIMADGNTAAALGSIFGGVQFVSWYPITPASSLAESLAEYLHLLRKDPETGKNTFGVV
                     QAEDELAAIGMAIGAGWSGLRAMTSTSGPGLSLMTEYAGLGYYSEVPIVVWDVQRVGP
                     STGLPTRTMQGDLTFVYFMGHGDTNQVILLPGTVSECFEFGWKSFDIAERLQTPVFVL
                     SDLDLGMNNWMTRPFEYPDKPIDRGKVLWEEDLKKLNGQWGRYKDIDGDGIPYRTLPG
                     NRHPQAAYMTRGTGHDEYAHYTEDPDEWHNMMERLKRKFETACKYVPA"
     gene            complement(1519..>2060)
                     /locus_tag="AC16V7_07875"
     CDS             complement(1519..>2060)
                     /locus_tag="AC16V7_07875"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR6257076.1"
                     /translation="LVDVRPRTGIRRKAYAFFPAVWQSLGYAVELDHAYFDAFAELRN
                     HLEAAYWHQAVRLLTPQDHLELQRLVANALEKLQGPQIQIPHSEHRQLHICIYSRLQN
                     PFVLGILEAYWQAYEAVGLNLYAGYDYLQEVWEYHRRMVDAICCGDFDGGYQALVEHT
                     DLLHHRPLSVLMGNSITEK"
BASE COUNT          520 a          550 c          488 g          502 t
ORIGIN      
        1 gagcgggcac atatttgcag gcagtctcga atttgcgctt cagccgctcc atcatgttat
       61 gccattcgtc tggatcttcg gtgtaatggg cgtactcatc atgtccggtt ccacgtgtca
      121 tataggctgc ctgcggatga cggttaccag gtaaggtgcg gtaagggata ccatcaccgt
      181 caatatcctt gtagcgcccc cattgaccgt tcagtttctt caggtcctct tcccagagaa
      241 ctttacctcg atcaatgggt ttgtccgggt actcaaatgg gcgtgtcatc caattattca
      301 tacccaaatc cagatcagat agcacaaaga caggtgtctg caagcgctca gcgatatcaa
      361 aggacttcca tccaaactcg aaacactccg acaccgtgcc aggtagtaag ataacctggt
      421 ttgtatcacc gtggcccata aagtatacaa aggtcaggtc gccctgcatt gtacgggtcg
      481 gtaagccagt gcttggaccc acacgttgca catcccatac gactatcggt acttccgaat
      541 aatatcccag accagcatat tcggtcatca agcttaatcc cgggccggag gtggacgtca
      601 tcgctcgcaa accggaccac cctgccccaa tcgccatgcc gatagctgct aattcatctt
      661 cggcctggac gacgccaaag gtattcttac ccgtttccgg gtctttgcgc agtaagtgaa
      721 ggtactctgc cagcgactcc gccaggctgg atgcgggggt aatcggatac caggagacga
      781 actgcacccc tccaaagata gagcctaaag cagcggcggt gtttccatca gccataatgt
      841 agtctgttgt ggcatccatt ggctcaacgc cgaagggatc cgtcttttct agattctgag
      901 ccgcccaatt ggccgacgcc tgaaccacct cccagttcat ttcaatgggt ttagttttgc
      961 ctttaaaatg aaagctgatt gcttgataga tcttatccag atcaatgccc agcatttgcg
     1021 ccaaaatgcc cacataaacc atgttagcga cataatccct caagcttgaa ggagcgccag
     1081 actcccggac aatttgtttt accggcattg cgtacgacac aatgtcgtct cggttgaccg
     1141 acatcttgat gtcatcggca tagaaaaaga cgccccctgg tgaaacatcg gccaaatctc
     1201 tggaaaaggt ggccgggttc atggccacaa cgatctcatg ctcttccctc cgggccagaa
     1261 agccatcctt gctcacgcgg atggtgtacc aggtcggtaa gccctgaatg ttcgaaggga
     1321 aaagattttt ccctgaagtt gggataccca tcttaaaaag ggcgcgcaaa atagtgagat
     1381 tggaagtttg gcttcccgaa ccattgaccg tggcaacggt gatcgagaaa ttatttatcc
     1441 gatcctcggt ctcagcacgg gattcctctt cggcaagcgt agttaatgca gtcatcacta
     1501 ctccttgaga tgaactaatt acttttctgt gattgaattt cccatcaaca ccgataacgg
     1561 gcgatggtgt aataggtctg tatgctccac tagagcctga tagccgccat caaaatcacc
     1621 gcagcatatc gcatccacca tgcgtcgatg atattcccat acttcttgca ggtaatcata
     1681 gccagcgtaa agatttaaac caacagcttc atatgcctgc cagtaggctt ccaaaatacc
     1741 cagcacaaaa ggattttgca gcctgctata gatacatata tgtaactggc ggtgttcgct
     1801 gtgagggatc tggatttgag ggccttgtaa tttttccaaa gcgtttgcca ccagcctttg
     1861 aagctcaagg tgatcttgcg gcgtaagcaa acggacagcc tggtgccagt aagcagcttc
     1921 caggtgatta cgtagctcgg caaaggcatc aaagtaggca tgatctagct caacagcgta
     1981 gcccaggctc tgccatacgg cgggaaaaaa ggcgtaagct ttgcggcgga taccagtacg
     2041 tggcctcaca tccaccaatc
//
LOCUS       DBNHOH010000450         1969 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1436665, whole genome shotgun sequence.
ACCESSION   DBNHOH010000450 DBNHOH010000000
VERSION     DBNHOH010000450.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1436665"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..1871
                     /locus_tag="AC16V7_07880"
     CDS             45..1871
                     /locus_tag="AC16V7_07880"
                     /EC_number="1.2.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HIR6257077.1"
                     /translation="MALGGYADKVAHIDLTKGQVTYDAIPEDWKLKYIGGRGVGVKYV
                     FDNGPQVDPLSPDNLLSFMNGPLTGTEVNLSGRIAIVTKSPLTGTITDSHHGGWSGAR
                     LRWAGFDGLNFKGKAEKPVYVFVKAGKVEIHDASDVWGKGVHDTVKYFKDKYGEKDLS
                     VITIGQAGEKLSRFACWVNENDRASGRGGTGCVGGSKNLKAVVIKATKKMPKAVDREL
                     WKEAHANALAQLKDPKYVTSPRKGGLSTLGTNVLMNMVNVIGAMPTKNSQLTYFEDHE
                     LISGEYVRDNILVNNPTCHACPVACKKEVIIKDGPYAGLQMESVEYESAWALGANCDN
                     SDVASVAKLIDQCNDYGFDTIEMGNVLSVYMEASEKGYTNGAGGLKWGDHPAMVETVR
                     KIASREGIGNVLAEGAERAAKTFGHPEIAMTVKGMAIPAYDPRGIKGMGIGFATSNRG
                     ACHLRGYTPAAEVVANVLGSTDLTDPLEWKGKGQLTVIFQNVHTMTDCLDICKFATFA
                     EPLDSFAAQYTAITGVPADAGMLLKAGERVYNLERYYNNLIGFVEGSDYLPERFLKEP
                     STGPGSKGHVCELPEMLEEYYEARGWRNGVVTEEKLKELELI"
BASE COUNT          524 a          473 c          538 g          434 t
ORIGIN      
        1 tatcgcacag aatttccatt caccattctc aaaaggaggc agatatggca ctcggagggt
       61 atgcagataa agtagctcac attgatttaa ccaaagggca ggtcacgtac gacgcaatcc
      121 ccgaagactg gaagcttaag tacatcgggg ggcgaggtgt gggcgtgaag tacgtctttg
      181 acaatggccc tcaggtagat cctctttctc ccgataacct tttaagcttt atgaatggtc
      241 cactgacggg tactgaggta aacctcagtg ggcgtatcgc catcgtcaca aagtcgccgc
      301 ttacgggcac catcaccgac agccaccatg gaggctggtc aggcgctcgt ttacgctggg
      361 caggctttga tggtttaaat ttcaaaggaa aagctgaaaa accagtatat gtttttgtca
      421 aggctggtaa agttgaaatt catgacgctt cagatgtctg ggggaaaggc gttcacgata
      481 cagtcaaata ctttaaggat aagtatggag aaaaagacct gagtgtcatc accattggtc
      541 aggctggcga gaaactctcg cggtttgcat gctgggtgaa tgaaaacgat cgagccagcg
      601 gtcgtggggg cacaggctgc gtgggaggca gcaagaacct caaagctgtc gtcatcaaag
      661 caaccaagaa gatgccaaaa gcggttgacc gcgagctctg gaaagaagct catgcaaacg
      721 ccctggctca actaaaggat cccaagtatg tcacatcacc gcgcaagggt ggtttgtcga
      781 ccctcggcac aaacgtgctt atgaatatgg tcaatgtgat cggcgccatg ccaaccaaga
      841 actcccagct gacctatttt gaggatcatg agttgatcag tggcgagtat gtacgcgata
      901 atatcctggt aaacaatccc acctgccatg cttgcccggt ggcttgcaag aaagaagtaa
      961 taatcaagga tgggccttac gccggcttgc agatggaaag tgtcgaatat gagtctgcat
     1021 gggcattagg cgcgaactgc gacaattccg atgttgccag cgtcgccaaa ttgattgacc
     1081 agtgcaatga ttacggtttc gataccatcg agatgggtaa tgtgctctcg gtctacatgg
     1141 aagccagcga gaaaggctat accaatggag cgggtggatt gaaatgggga gaccatcccg
     1201 ccatggtaga aacagtccgt aagatagcct cacgggaggg tattggtaat gtcctggctg
     1261 agggtgcgga acgagcggcg aagacattcg ggcatccaga aatagccatg accgtcaaag
     1321 gtatggcaat cccggcgtat gaccctcgcg gtatcaaggg tatgggcatc ggatttgcga
     1381 cgagcaaccg gggtgcctgc catctgcgcg gttacacccc ggctgccgaa gtggtcgcca
     1441 atgtacttgg ttcaacagat ctgaccgacc cgctggagtg gaaaggcaag gggcaactca
     1501 cagtgatctt tcaaaatgtc cacaccatga cagattgttt ggatatttgc aagttcgcta
     1561 ccttcgctga acccttggat tcgtttgccg cccaatacac agctatcaca ggcgtgccag
     1621 ccgatgccgg catgcttctc aaagcgggcg aacgcgtgta taacctggag cgctactata
     1681 acaacctaat aggattcgta gaaggctccg actacctgcc cgagcgcttt ctgaaagaac
     1741 cttccacagg ccctggctcg aagggacatg tttgcgagtt gcctgagatg ttggaagaat
     1801 actacgaagc gcgcggctgg agaaacggtg ttgtcaccga agagaagctc aaggagttag
     1861 agctcattta gccgtattaa aaaatacata acacatcccg aatttaatat tcggcacgtg
     1921 ctacttattt accccatccc cccttcccac cgctttgctc cgggaaggg
//
LOCUS       DBNHOH010000451         3807 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_281889, whole genome shotgun sequence.
ACCESSION   DBNHOH010000451 DBNHOH010000000
VERSION     DBNHOH010000451.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_281889"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1439
                     /gene="murC"
                     /locus_tag="AC16V7_07885"
     CDS             <1..1439
                     /gene="murC"
                     /locus_tag="AC16V7_07885"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR6257078.1"
                     /translation="GLIPVCSGGLYLMMNQSMEHVHLIGIGGSGLSAIARVLLERGVV
                     VSGSDRQPSGVTRALQDAGARVFIGHSAGNVQGADLVVRSSAVSDENLEVIAAQGAGI
                     PVLKRAEFLGQILEGYRVIAVAGTHGKTTTTAMLSWILTTLGESPSFIIGGMARDLGT
                     NARAGSSRLFVVEADEYDHMFLGLSPDIAVVTNVEHDHPDCYPTAEDFYRAFQQFAGR
                     LKPHGILVGCHDDPGARRLLRDAVQTGQRTLSYGLNHWAPLDGPNYAARELVPNARGG
                     FSFEVIQQDRQSAGIQVDLKIPGRHNVSNALACLVVIDCLNLPLEKAAQALGQYSGAG
                     RRFEVRGEARGVIVIDDYAHHPSEIRATLAAARSRFPEKNIWAVWQPHTYSRTRTLLS
                     EFATAFENADHVVVTDIYAARENSPANGFSSQQVVENMVHREANYVPGLSQAADYLLD
                     RVQPEAVVLVLSAGDADQISDLLLRKLG"
     gene            1487..1669
                     /locus_tag="AC16V7_07890"
     CDS             1487..1669
                     /locus_tag="AC16V7_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257079.1"
                     /translation="MTEKQRKYEAEVQLVKPQILMPESKHEEASMYLVNTRLERVHRT
                     FSRETVVRLINWFKQE"
     gene            1676..2644
                     /gene="murB"
                     /locus_tag="AC16V7_07895"
     CDS             1676..2644
                     /gene="murB"
                     /locus_tag="AC16V7_07895"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR6257080.1"
                     /translation="MFIRFETANVNLESLNRLQASFGQRLQRGVPLDRFTSARVGGTA
                     EILLEVESADELAQAATLLWSEGVAWKILGGGSNVLVSDSGVRGVVVVNRARKVRFDE
                     DSDPPTVWAESGANFGLVARQAASRGLSGLEWAAGIPGTIGGAVVGNAGAHGGDMAGN
                     LIVATILQRNLEDGVPIRVTWPVKRLEYAYRTSQLKRMPGQAVVLEAQLRLQRSTPEG
                     VQAKMDEYVSYRRRTQPPGASMGSMFKNPPGDYAGRLIEAAGLKGVQVGDAQISTLHA
                     NFFTNHGEATAADIYALIELAKRAVAEKFGVHLELEIELVGNWVGR"
     gene            2646..3767
                     /locus_tag="AC16V7_07900"
     CDS             2646..3767
                     /locus_tag="AC16V7_07900"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR6257081.1"
                     /translation="MSQKDKLRVGVLFGGRSGEHEISLLSARSVLSALDPQKYEVTQI
                     GITHQGVWLVGENVLVAFNQGHKKDLVSAALLPVPTRNDLYAIRPTQHGEIFELISKL
                     DVVFPILHGTFGEDGTIQGLFEMADLAYIGAGVVGSSVGMDKGIFKDVMRSNAIPVLE
                     YVIVLRREIENAMEAVIAKAEALASYPLFVKPANLGSSVGVSKCSNRSDLYEGLIDAA
                     RYDRRVLVERGINAREIEVSVLGNDDPQASVVGEVIPSRDFYSYEAKYLDDRSELLIP
                     APISKELSQQVQSLAVQSFKAIDCAGLARVDFLLDKDTNELYLNEINTIPGFTRISMY
                     PKLWDAAGVPYPALVDRLIDLALERKRERDRTVRKYNRV"
BASE COUNT          858 a          871 c         1133 g          945 t
ORIGIN      
        1 gcggtctcat tcctgtttgt tctggtggtc tttatttaat gatgaatcaa tcgatggagc
       61 acgtgcatct cattggtatc ggcggaagcg gcctttcggc cattgcccgc gttcttttgg
      121 aacgcggcgt agtggtcagt ggctccgacc ggcagccttc tggggtaacc cgggcgctgc
      181 aggacgctgg tgcacgtgtg ttcatcggcc acagtgcggg taatgtacaa ggggcggacc
      241 tggtggtacg ctcatcggct gtctcggatg agaatcttga agttattgct gcgcaaggag
      301 ccggcatccc ggtgcttaag cgagccgaat tcttgggaca aatcctggaa ggctaccggg
      361 tgattgcagt tgccggcacg cacggcaaga ctaccaccac ggccatgctg agctggattt
      421 tgacaacgct cggagaatct ccatctttta tcatcggtgg catggcgcgc gatttgggca
      481 ccaatgctcg agcaggctca agtagattgt ttgtcgtcga agccgatgag tacgaccaca
      541 tgttcttagg cctttcgcct gatatcgctg tggtgaccaa cgtggagcat gatcatccgg
      601 attgctatcc gacagcggag gacttttacc gggctttcca gcagtttgcc ggtcgtttga
      661 aaccgcacgg cattttggtg ggctgtcatg atgatcctgg cgcccggcgc ttattaaggg
      721 atgctgtaca aactggtcaa cgcaccttgt catatggctt gaatcactgg gcacccttgg
      781 atggacccaa ctatgcagcc cgggagctag tgccaaatgc gcgtggcggt ttcagcttcg
      841 aggtgatcca acaggatcgt caatcagcgg gtatccaggt ggatttgaag atccccggac
      901 ggcacaatgt cagcaacgcg ctggcttgct tggttgtgat cgattgtctt aatctccctc
      961 tggagaaggc tgcccaggcg ctcggacagt atagtggcgc aggaagacgt tttgaggtgc
     1021 gcggagaagc ccgtggtgtg atcgtcattg atgattacgc ccaccatcca agtgagattc
     1081 gggcaacgct ggctgcagct cgttcacgct ttccagagaa aaacatctgg gctgtctggc
     1141 aaccacatac ttattcgcgc acgcgcaccc tgctctccga gttcgccacg gcgtttgaga
     1201 acgctgacca tgttgtggtt acggatattt atgcggcgcg ggagaactct cctgccaatg
     1261 gcttttcatc gcaacaagtt gtggaaaaca tggtgcaccg agaagccaat tatgtccccg
     1321 ggctatctca ggcggctgat taccttctgg atcgtgtcca gcctgaggct gtcgtgctgg
     1381 tgctctccgc tggagacgct gaccaaatca gtgatttatt gctgcgcaag ttgggatgag
     1441 ctaattgaat cgttatagca gccccgtagt tgtaggagtg acgaggatga ctgaaaaaca
     1501 acgcaagtat gaagctgaag tgcagttggt caagccgcaa atattgatgc cggagtcaaa
     1561 acacgaagag gcgagcatgt acctggtgaa tactcgtctc gaacgtgttc ataggacatt
     1621 ctcgcgggag actgtcgttc gcttgatcaa ttggtttaag caggaataac gtgctttgtt
     1681 cattcgattt gaaactgcca acgtgaacct cgaaagcctt aaccgtctgc aagcctcttt
     1741 tggacagcgc ctgcaacgcg gtgtgccatt agaccgcttc acctcggctc gggttggcgg
     1801 cacggcggag atcttgctgg aggtcgaatc agctgatgag ctggcgcagg cagctacgct
     1861 tctatggagt gagggtgtag cttggaaaat actgggcggt ggctccaatg tgctggtgag
     1921 cgactcgggt gtacgtggcg tggtggttgt caaccgggca cggaaagtgc gttttgatga
     1981 ggactctgac ccaccgacag tgtgggctga atcaggtgca aactttggtt tggtcgcccg
     2041 gcaggctgcc agccgtggat tatcgggatt ggaatgggcg gcgggcattc ccggcacgat
     2101 cggcggagcg gtagtaggta atgctggtgc ccatggtgga gatatggctg ggaatctgat
     2161 cgtggcaacc atcttgcaac gaaatctaga ggatggggta ccgattcgag tgacctggcc
     2221 tgtaaaacgg cttgagtatg cttatcgtac aagccaactg aagcgtatgc cggggcaagc
     2281 ggttgtttta gaggctcaac tacgtttgca gcgcagtacg cctgaagggg tacaggcaaa
     2341 gatggatgaa tacgtgagct atcgccgtcg cacacaaccc ccaggtgcaa gtatgggctc
     2401 tatgttcaaa aatcccccag gagactacgc cggtcgtctg attgaagctg ctgggttgaa
     2461 aggtgttcaa gtaggtgatg cgcaaattag tactttgcat gccaattttt tcactaacca
     2521 cggagaggcc actgctgctg atatttatgc actgattgag ctagcgaaaa gagctgttgc
     2581 tgagaaattc ggtgtgcacc tggaactgga aatcgaattg gtaggaaatt gggtaggtcg
     2641 ataagatgag tcaaaaagat aaattgcgcg tcggggtgct ttttgggggg cggtcgggag
     2701 agcatgaaat ctctttgctc tcagcccgtt cggtgttgtc ggcgctcgat ccgcagaaat
     2761 acgaggtaac ccagattggg ataactcacc agggtgtctg gttggtagga gagaatgtgc
     2821 tggttgcgtt taaccaaggc cataaaaagg atttggtttc tgcagcgctc ttgcccgtcc
     2881 caacccgtaa cgatctatat gctatccgcc cgacacaaca tggcgagatc ttcgaattaa
     2941 tctccaaatt ggatgtggtt ttcccaatcc tgcacggtac atttggagag gatggcacta
     3001 tccagggtct atttgaaatg gcagatctgg catatatagg ggctggcgtg gtgggttcat
     3061 cggtaggaat ggacaagggt attttcaaag atgttatgcg ttcaaatgct atcccagtat
     3121 tagagtacgt gattgtgctg cgccgcgaga tcgagaacgc catggaagcc gtcattgcca
     3181 aagccgaggc cttggcctct tatccgttgt ttgtcaagcc agcaaacctg ggatcatcag
     3241 taggggtaag taaatgcagc aatcgttctg atctatatga aggcttgata gatgctgctc
     3301 gttacgacag gcgggttctg gtggagcgag gcatcaacgc tcgcgagatc gaggtgagtg
     3361 ttttgggcaa tgatgatccg caggcatctg tggtcggaga ggttatcccc tcgcgtgact
     3421 tctattctta tgaggccaaa tatctggatg accgctctga attgctgatc cctgcgccta
     3481 tttcaaaaga actaagtcag caggttcaat ccctagcggt acagtcattc aaggcgattg
     3541 attgcgccgg gctggcacga gtggattttt tactagataa agatacgaat gagttgtatt
     3601 tgaatgagat caacactatt ccaggattca cccgcatcag catgtacccc aagttgtggg
     3661 atgctgccgg cgttccttat ccagcactgg tggatcgatt gattgatttg gcattggagc
     3721 ggaaaagaga acgggaccgc actgtgcgta agtataaccg ggtgtaacag cgattggaga
     3781 atatcgttat gtcagaaacg agtcgct
//
LOCUS       DBNHOH010000452         1720 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_410432, whole genome shotgun sequence.
ACCESSION   DBNHOH010000452 DBNHOH010000000
VERSION     DBNHOH010000452.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_410432"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..781
                     /locus_tag="AC16V7_07905"
     CDS             221..781
                     /locus_tag="AC16V7_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257082.1"
                     /translation="MVSTETIERAHDPVEALNASLERAWHSLNQAYDSTAFMPQLPEL
                     FARADLSRPEQAAQAVLVSMLHEVMETAGRFSPWYKQPARAFGLTSVRDPLTQRPRWL
                     LAPEAAQRWRRVLESLSDVISQYAGLIQATILVDDLMSDLPANDPRIIASCTCRPPRT
                     IQLRQSVMDKAEILCQNCSQPFLERG"
     gene            1064..>1720
                     /locus_tag="AC16V7_07910"
     CDS             1064..>1720
                     /locus_tag="AC16V7_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004066796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6257083.1"
                     /translation="MSHAYMGKILRVNLTKGEIIEEELREQWVREFIGGAGLATKYLY
                     EEVPKGVDPLGPDNRLIFMTGPLTGTSSASASRYSVVAKSPLTGIWGQANSGGSFGPA
                     LRRSGYDGIIFEGASAIPVYLRIIRGRAELCDASHLWGKTISETEDLIQQSSEQKLTV
                     ASIGPAGENLVRFAAIMNNTHRAAGRCGLGAVMGSKRLKAIACAGNSPVPLANEDLFR
                     E"
BASE COUNT          431 a          468 c          473 g          348 t
ORIGIN      
        1 gagaaggaag cggctctgca taaaaacctg gtagacccaa ggccgctgtt atatgctaaa
       61 gggatgatcg gaacatcgtg aattctccag gcggctttgc cgtctcggag gggcgtcgaa
      121 gtactcctcc cacaccaccc tctctctcaa agaggaggca gactccaagg ttctagagaa
      181 ccagtcaggg ttcgaggagc ccaccagagg aggtcttcgc atggtttcaa cggaaacaat
      241 cgaacgggca catgatccgg ttgaggcgct caacgcctct ctagagcgcg cctggcatag
      301 cctgaaccag gcatacgaca gcacagcttt tatgccccag ctgcccgagc tgttcgcgag
      361 ggcagatctt tccaggccag agcaggccgc tcaggcagtg ctggtctcaa tgctccatga
      421 ggtgatggag acagcgggtc gcttctcacc ctggtataag caacccgcgc gggcattcgg
      481 gttgacttcg gtgcgcgatc cgctaaccca gcgaccgcgc tggctcttgg ctcccgaggc
      541 agctcagcgc tggcgaagag tattggaatc gcttagcgat gtaatcagcc agtacgcagg
      601 tctcatccag gctacgatcc tggtggacga tctgatgagc gacctgcctg ctaacgaccc
      661 ccgcatcatc gccagctgca cctgtcggcc gccgcgcacg atccagcttc gccagtcagt
      721 gatggacaag gcagagatct tgtgccagaa ctgctcgcaa ccgtttttag agagagggtg
      781 atttattatc aaaatgggcg gaaactatag gtgggcggtt gccataaata aagtgacaaa
      841 ccgccgatat aagaagccag actaaactta gagagacaca cgctacatgc tggctaaagt
      901 tgccccaacc cacattgcaa aatgaatcgt cgagcacact aggctgagaa attgtccaac
      961 acaatctcct aaaccaactc aatacgaacc ttaagttcac acaatagacc gtgtaagtag
     1021 cgataataaa aaatcttcaa tttgccttgg aggagataaa acaatgagcc acgcgtatat
     1081 ggggaaaatc ttgcgggtaa acctgaccaa gggagaaatt atcgaagaag agcttcgcga
     1141 gcaatgggtc agggagttca ttggtggcgc cgggttagca accaaatacc tctacgagga
     1201 ggtccccaaa ggggttgatc ccttagggcc cgataaccgg ctgatcttca tgaccggtcc
     1261 ccttaccggc acatcatctg ccagtgccag ccggtattcg gtggtggcga aatctcctct
     1321 gaccggcatc tggggacaag ccaattcagg gggcagtttt ggccctgccc ttaggagaag
     1381 cggatatgac ggcatcatct tcgagggggc atctgctatt cccgtctact taaggatcat
     1441 acgcggtcgc gccgagctgt gcgacgcctc ccacctgtgg ggaaagacga tctctgaaac
     1501 cgaggatttg attcagcaaa gttctgaaca aaaattgact gtcgcctcca ttggaccagc
     1561 cggagaaaac ctcgtccgct tcgccgctat tatgaacaac acacaccgcg ccgccgggcg
     1621 gtgcggttta ggcgcggtga tgggatccaa gcggttgaag gctattgcct gcgcggggaa
     1681 ctcccctgtt cccctggcaa acgaagatct ttttagggaa
//
LOCUS       DBNHOH010000453         2435 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_859719, whole genome shotgun sequence.
ACCESSION   DBNHOH010000453 DBNHOH010000000
VERSION     DBNHOH010000453.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_859719"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="AC16V7_07915"
     CDS             complement(<1..852)
                     /locus_tag="AC16V7_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257084.1"
                     /translation="MRTTRLLNILGVPLAFLLLLLLSFGLMIPWLGFYWDDWPIMLAT
                     RLQGIGAFWEYYGDERPFNPLTVIPSAWLMGTQPLNWHIFALLMRWLTVLGIYWSLKR
                     LWPRQVWQVTGMVLLFAIYPIFTQQPVSATFTTHWIVYASFFYSLGAMIQSERTPRWR
                     WPLTLTGIAAQLLHMLPLEYFWGLELLRPFMLWLIHTEKIPDKRKLMWATLRSWAPYL
                     LVYSGVVAWWVSYRGNLPDSNQPDALLSLGATPLQTGFHFIQIILQDSIHNLLGAWYQ
                     TINPEGLD"
     gene            991..1974
                     /locus_tag="AC16V7_07920"
     CDS             991..1974
                     /locus_tag="AC16V7_07920"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR6257085.1"
                     /translation="MKQSHIFITRKIPMEKLALARQLGQVDLWDDDLPPSPQALLEHI
                     QGVDAILCLLTERIDAEVMDTAGPQLKVISNCAVGYDNIDVAEANRRRIPVGNTPGVL
                     TDTTADVAFALLMSAARRIVEGNQFARQGQWKTWGLTQLLGQDITGATLGIIGFGRIG
                     KGMAKRARGFDMRVLFYDPFGQDDPYAAEVGAQAVDLDTLLRQSDFITVHSPLNRETY
                     HLIGEGALAKMKPNAILINTARGPVVDPQALYHALKDGVILGAALDVTDPEPIPANDP
                     LLSLENVIITPHIASASVETRGRMVEMALDNLRAGLRGERLPNCVNPEVYR"
     gene            complement(2009..>2435)
                     /locus_tag="AC16V7_07925"
     CDS             complement(2009..>2435)
                     /locus_tag="AC16V7_07925"
                     /inference="COORDINATES: protein motif:HMM:NF015791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HIR6257086.1"
                     /translation="HQLGILRHMGVQVEPAESTEQASDSELVIDGLIGYSLQGTPRGS
                     DAALIRWANAQPTPILALDIPSGLDATSGAAYNPTIRATATMTLALPKEGLRTEAAQA
                     YTGELYLADISVPPGLYSGPSLGLQVWPIFAENEIIRLI"
BASE COUNT          562 a          628 c          727 g          518 t
ORIGIN      
        1 atccagaccc tctgggttga tggtctggta ccatgcaccc aacaggttgt ggatcgagtc
       61 ttgcaggatg atctggataa aatgaaaccc ggtctgtaag ggggtagcgc ccagggaaag
      121 gagtgcgtct ggttggttag aatcgggcaa atttcccctg tacgagaccc accaggcaac
      181 caccccactg tagaccagca gatagggggc ccaggatctc aatgtagccc acatgagctt
      241 gcgtttatcc gggattttct ctgtatggat gagccatagc atgaatgggc gcagcagctc
      301 cagtccccag aaatattcta acggcaacat gtgcaggagt tgggctgcta tgccagttaa
      361 agtgagcggc cagcgccagc ggggtgtgcg ttcagattga atcatggcac ccagagaata
      421 aaagaatgag gcgtagacaa tccaatgcgt ggtaaatgtg gctgatacgg gctgttgggt
      481 gaagatcggg tatattgcaa ataaaagcac catccctgtg acctgccaaa cctggcgcgg
      541 ccataatctc ttcaggctcc agtatattcc tagcacggtc aaccagcgca tcagtagggc
      601 aaagatgtgc caattcaggg gctgcgtgcc cattagccag gcggatggaa tgactgtcag
      661 gggattgaac ggtcgttcat ccccgtagta ttcccaaaat gcaccgatcc cttgcaggcg
      721 ggtcgccagc atgatcggcc aatcatccca atagaagcct aaccagggga tcattagccc
      781 aaagctgagc agtagtaaaa gcagaaaggc gagcgggacc cctaaaatat tgagtagtcg
      841 cgttgtgcgc atctatgtat tataaacaga tgttatgaag ttggggaagt ggtgggattc
      901 gggggctgcc aaacttgtca atccggcgat gacgttttac aatacaagaa agcccggata
      961 aacattttgg tgcgatttag ccgaggttca atgaagcaat cccatatttt cataacccgc
     1021 aagatcccga tggaaaagct cgccctggca cgccagctag ggcaggtgga cttgtgggat
     1081 gatgatctgc cgccctcgcc gcaagccctt ctggagcaca ttcaaggggt agatgccatc
     1141 ttgtgcttgc tgacggagcg catcgacgct gaggtaatgg atacggccgg cccgcagctc
     1201 aaggtgataa gcaactgtgc tgtggggtac gataatatcg atgtagccga ggcaaaccgg
     1261 cgacgaatcc cggtcggcaa cacgcccggt gtgctcaccg ataccaccgc cgatgttgcc
     1321 ttcgcattac tgatgtccgc ggcgcggcga atagtcgagg ggaatcaatt tgcccgccag
     1381 ggccagtgga aaacctgggg actgacgcag ctactcgggc aggatatcac gggggccacg
     1441 ctgggcatta tcggttttgg gcgcatcggc aaggggatgg caaaacgtgc ccgcggtttt
     1501 gatatgcgcg tcctgtttta cgatccgttt ggccaagatg atccttatgc tgctgaggta
     1561 ggggcgcaag ctgttgattt ggacaccctg ttgcgccaat ccgattttat tacggtacac
     1621 agcccgctta atcgagagac ttatcatctg atcggcgagg gggctttagc caaaatgaag
     1681 cccaacgcca tcctaatcaa taccgcgcgc ggcccggtgg tcgatccgca ggcgctctac
     1741 catgcgctaa aggatggcgt gatcctcggc gcagccctgg atgtcactga tccggagcca
     1801 atcccggcca atgatccctt gctgagcctg gagaacgtga tcatcactcc gcacattgcc
     1861 agcgccagtg tagagacgcg cggcaggatg gtagagatgg cgctggataa cttgcgtgcg
     1921 ggtctgcgcg gcgagcgctt gcccaactgc gttaacccag aggtgtatcg ataagggggc
     1981 aatgggtgct gggtgcccga tacccgtttt atattagccg gataatctca ttctcagcaa
     2041 aaattggcca gacctgcaag cccaggcttg gtccgctata caaacctggt ggcacgctga
     2101 tatcggccag atacagctcg ccggtgtaag cctgcgcagc ttcagtgcgc aagccttctt
     2161 tgggcagagc cagggtcatg gtagcggttg cgcggatcgt gggattatag gctgcgccgc
     2221 tagtggcgtc caaccccgag gggatatcca aagccaggat gggggttggt tgggcattcg
     2281 cccagcggat cagcgccgcg tcggaaccac gcggggtgcc ttggaggcta tacccgatca
     2341 ggccatcgat gaccagttcg ctatcgctgg cttgttcggt tgattcggcg ggctctacct
     2401 gcacacccat atgccgcaag atgcctaatt gatgg
//
LOCUS       DBNHOH010000454         1388 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_475238, whole genome shotgun sequence.
ACCESSION   DBNHOH010000454 DBNHOH010000000
VERSION     DBNHOH010000454.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_475238"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="AC16V7_07930"
     CDS             <1..531
                     /locus_tag="AC16V7_07930"
                     /inference="COORDINATES: protein motif:HMM:NF024785.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HIR6257087.1"
                     /translation="SLSTLSGGLSVLAAAREVLAGNGAVVEALEQLERVATAVQARFP
                     DVALYLDLAELRGYNYHTGLVFAAYLPGYGQAVAKGGRYDEIGKVFGRARPATGFSAD
                     LKVLVEQAQTPDAGVQGILAMGRGDESLTAEVARLRAIGERVVIALPGAEADAADMGC
                     DRKLIQADGDWIVTAI"
     gene            568..>1388
                     /locus_tag="AC16V7_07935"
     CDS             568..>1388
                     /locus_tag="AC16V7_07935"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIR6257088.1"
                     /translation="MGKNVVVLGTQWGDEGKGKIVDLLTEQAVAVARFQGGHNAGHTL
                     VIDGEKTVLHLIPSGILRDNVTCMIGNGVVLSPEALLKEIGELESKGVPVRERLRLSP
                     ACPLILSYHVALDQARELARGENKIGTTGRGIGPAYEDKVARRGLRLGDMLYPERFAV
                     KLKEVLEYHNFALTQYYKVDAVDYQSTLDEAMQMAEELRPMVADVTAMLHDYRRKGEN
                     ILFEGAQGSLLDIDHGTYPFVTSSNPTAGGTATGSGFGPLYLDYILGITKAYTTRV"
BASE COUNT          278 a          339 c          446 g          325 t
ORIGIN      
        1 tcattgtcga cattgagtgg cgggctgtca gtgttggctg ctgcacgtga agtgttagcc
       61 ggtaatggag cggttgttga agcgctggag cagctcgagc gggttgcaac agcggtgcag
      121 gctcgattcc cggatgtcgc actgtatttg gatctggctg agttgcgcgg atacaactac
      181 cacaccggat tggttttcgc cgcttacttg cccggttacg ggcaggcggt cgccaagggt
      241 ggtcgttacg atgagattgg caaagtattt ggccgagcgc gtcctgctac tggcttcagt
      301 gcggatctga aggtgctggt tgagcaggcg caaaccccgg atgccggagt acagggaatt
      361 ctggcaatgg gacgtggtga tgaatcgctt accgcggaag tcgctcgttt gagggctatc
      421 ggtgagcgtg ttgtcatcgc tttgccgggt gcagaagcgg acgcagcaga tatggggtgt
      481 gaccgcaaac tgattcaggc tgatggcgat tggatcgtca cggctatcta aatttattaa
      541 ctgtttacgt ctagagaatt accgaatatg ggcaaaaatg tcgttgtttt aggcacccag
      601 tggggcgacg agggtaaagg caaaatcgtt gacctgttga ctgagcaggc ggttgccgta
      661 gcgcgctttc aaggcgggca caatgcgggg catacgctgg ttattgacgg cgaaaagacc
      721 gttctgcatc tgataccctc cggtattttg cgtgacaatg tcacctgcat gatcggtaat
      781 ggtgtggtgc tatctcccga agcgttgctc aaagagatcg gtgagctcga gtccaaaggc
      841 gttccggtgc gcgagcgtct ccgcttgagc cccgcgtgcc cgctgatttt gagctatcac
      901 gtggcgctgg atcaggctcg tgagctggct cgtggcgaaa acaagatcgg taccacgggt
      961 cgtggcatcg gacccgctta cgaagataaa gtcgcgcgcc gcgggttgcg gcttggcgat
     1021 atgctctacc ccgagcggtt tgcggtcaag ctcaaagagg tactggaata ccataacttc
     1081 gccttgaccc agtactacaa ggtggatgct gtggattacc agagcacgct ggatgaagcg
     1141 atgcagatgg ccgaagaact gcgtccgatg gtggcggatg tgaccgccat gctgcacgat
     1201 tatcgccgca agggtgagaa tatcctgttt gagggtgccc agggttcgct tctggatatc
     1261 gaccacggta cttatccgtt cgtgacctcc tccaatccca ccgcgggcgg caccgcgacc
     1321 ggcagcggtt ttggcccgct gtatctggat tatatccttg gcatcaccaa ggcctatacc
     1381 acgcgggt
//
LOCUS       DBNHOH010000455         3194 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1180127, whole genome shotgun sequence.
ACCESSION   DBNHOH010000455 DBNHOH010000000
VERSION     DBNHOH010000455.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1180127"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="AC16V7_07940"
     CDS             complement(<1..399)
                     /locus_tag="AC16V7_07940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR6257089.1"
                     /translation="MKHAVIIGAGMGGLATALRLSYHGFRVTVLEKQPRPGGRSNVIE
                     ESGFRADMGPTILVMKGAFEELYRSIGQDLNSRLQFIQLDPNYRVYYHDGSCIDLYSN
                     MAKLAQEIERVQSGGAERLFRFVGEGAKKYQ"
     gene            complement(585..2009)
                     /locus_tag="AC16V7_07945"
     CDS             complement(585..2009)
                     /locus_tag="AC16V7_07945"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR6257090.1"
                     /translation="MAKNPRQKQTFVTKKHLARQQREQRSIRMITISAIVVIVAVIGL
                     IGFGVLNERVLKGLQPVAIVNGEKITTNEFQAQARLARQNLVDNALSTYQFAQQFGNN
                     PQFLSSLSSQLSQIQSQLEPVILGNQVLEQMIQDRLIRQEASRRGITVSQEEIEQSVQ
                     EAFGYYQNGTPTPEPTLAEKPTSTLSSLQMTLTAPTATLAFSPTFAATQAFTPTATLE
                     PTVTPTATATITPIPSPTPTQAPTATPTEYTLEGYQQLYKDQLERLKSIDFDEDDLRY
                     IIESQLYREKVLDAVLAESDLTAEEDQVWARHILVPDEATANIVLGRLADGEDWSELA
                     ALFSTDTSNKDFGGDLGWFGKGRMVAEFENAAFALDVGETSQPVQTSFGWHIIQVLGH
                     EARPLSQDEYEQLRQTRFDEWIKEQRDATEVETLEIWQERVPTEPAFPAEVEGFIQAV
                     QQQLLQQSQPTVPAPSVTEEAPTQ"
     gene            complement(2078..2350)
                     /gene="rpsR"
                     /locus_tag="AC16V7_07950"
     CDS             complement(2078..2350)
                     /gene="rpsR"
                     /locus_tag="AC16V7_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR6257091.1"
                     /translation="MSNQFDMKDQMQSGPRRYYAHPRVCQFCTDHNVTIDYKNVDLLR
                     RYVTEEGKIRPRRQTGTCARHQRELARAIKRARHLALLQFTGEILR"
     gene            complement(2369..2764)
                     /locus_tag="AC16V7_07955"
     CDS             complement(2369..2764)
                     /locus_tag="AC16V7_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR6257092.1"
                     /translation="MSRGLNKVMLIGRLGRDPEMRYTPSGRPVTTFSLATSRTWNTSE
                     GERRTETEWFNIVAWGSLAEICKQFLNKGQQVYVEGRLQTRHWEDAEGSKHSVTEIVA
                     NEMIILGDRREMNNAAGEPGAEEEDEFPF"
     gene            complement(2799..3080)
                     /gene="rpsF"
                     /locus_tag="AC16V7_07960"
     CDS             complement(2799..3080)
                     /gene="rpsF"
                     /locus_tag="AC16V7_07960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR6257093.1"
                     /translation="MREYEYIFIIHPDLDENAFKELVDKIKGWITGAGGQVVKVDLWG
                     KRKLAYPIRKQTEGQYVLLDTVMSPDYSAELERNVRFLEPILRYLVTLK"
BASE COUNT          743 a          865 c          779 g          807 t
ORIGIN      
        1 ctggtatttt ttggctccct caccaacaaa acggaaaagg cgttctgcac cccccgattg
       61 caccctttca atctcctgcg ccaatttagc catgttgctg tacaaatcaa tacagctccc
      121 gtcgtgataa taaacccggt agttgggatc tagctggata aattgtagcc gggaattgag
      181 atcttgacca atggagcggt acagctcctc aaaggcacct ttcatcacaa ggatggtagg
      241 ccccatatct gcgcggaaac cggactcctc gatgacgttg ctccgcccac ctggacgggg
      301 ttgtttttcc agcacggtaa cacgaaagcc gtgataactc agccgcaagg ccgtagccag
      361 cccccccata cccgcgccaa tgatcacagc gtgtttcata gccatttcct ttgatgaaga
      421 tagataaatt ctatcctatt gcacatataa tgtcaatata ttattgacat ttattgtaca
      481 taagacctct cgatctcgcc gttataaaaa aactgggtgt gaacaagcaa ttcacaccca
      541 atttattcga catagagagc aattgtagga aaaatatttt ccagctattg cgtcggggct
      601 tcctccgtca ccgaaggagc tggcacagtg ggttgagatt gctgtaagag ttgctgttga
      661 acggcttgaa taaagccctc cacctctgcc gggaaagccg gctcggtggg gacgcgctcc
      721 tgccaaatct ccaaggtttc aacctcggta gcatcgcgtt gttctttaat ccattcatca
      781 aatcgagttt gacgtaactg ctcgtattca tcttgtgaga gtggtctcgc ctcatgacca
      841 agcacctgaa tgatgtgcca gccgaagctg gtctgcactg gctgacttgt ctcaccaaca
      901 tctaaagcaa aggcagcatt ctcgaattca gccaccatgc gaccctttcc aaaccatcca
      961 agatcgccac caaaatcttt attgctcgta tcggtagaaa acagggctgc aagctcgctc
     1021 cagtcttccc catcggcgag ccgacccagc acgatgttag ccgtagcctc atcaggcacc
     1081 aggatatgcc gggcccacac ctgatcctcc tccgctgtta aatcagattc tgcaagaacc
     1141 gcatctaaca ctttctcgcg atagagctgg ctctcgatga tatatcgcag atcatcttca
     1201 tcaaaatcaa tcgacttcag cctctcgagt tggtccttat agagctgctg ataaccctcc
     1261 agcgtatact cggtgggtgt tgctgtcggc gcttgcgttg gagtggggct tggaataggc
     1321 gtgatggttg ccgtagcggt gggtgtgacg gtaggttcca aggtcgccgt gggtgtaaaa
     1381 gcctgcgtgg cagcgaacgt tggcgagaaa gccagggtcg ccgtgggagc cgtcagggtc
     1441 atttgcaatg aggagagtgt tgaggtgggt ttttcggcca gggtgggctc gggtgtaggt
     1501 gtgccatttt gataataacc aaaagcctct tggacagatt gctctatctc ctcttgactc
     1561 acagtgatac cacgccggct ggcctcctgt cggatcaacc gatcctggat catttgctcc
     1621 agtacctggt tgcctaaaat cacgggctca agctgcgact ggatttggga taactggctc
     1681 gacagcgatg agagaaattg agggttattg ccgaactgtt gcgcaaactg gtatgtgctg
     1741 agcgcattgt caactagatt ttgtcgcgcc aggcgcgctt gcgcctgaaa ttcgtttgtg
     1801 gtgatcttct ctccattaac tatcgccacc ggctggagac ctttcagcac gcgctcattg
     1861 agcacgccaa aaccgatcaa cccaatcaca gccacaatta ccacaatcgc gctgatggtg
     1921 atcattcgaa tcgatctttg ctcgcgctgc tggcgggcaa gatgtttctt ggtaacaaac
     1981 gtttgtttct gtctaggatt ttttgccatg ttaaatctac aagaaagaag cgccagtgtc
     2041 taaatcaccc atctgacgct tcgtggagat tcggatttca gcgcaggatt tcgccagtaa
     2101 actggagcag agccaggtgg cgggcgcgct tgatggcacg cgccagctcg cgttggtgac
     2161 gagcacaggt gcctgtctgt cgacgcgggc ggatcttgcc ctcttcagta acgtaacgcc
     2221 tcagcaaatc gacgttcttg taatcaatag tgacgttatg gtcagtacaa aactggcaca
     2281 cgcgtgggtg agcatagtaa cgtcgcggcc cgctttgcat ctggtctttc atatcaaatt
     2341 ggttactcac gattaaaact ccttattctt aaaagggaaa ttcgtcttcc tcttccgccc
     2401 cgggctctcc cgccgcattg ttcatctcgc ggcggtcgcc aagtatgatc atctcattgg
     2461 caacgatctc cgtgacagaa tgcttgctcc cttcagcgtc ttcccaatgg cgggtttgca
     2521 agcgcccctc aacatacact tgttggcctt tattgagaaa ctgcttacaa atctctgcca
     2581 ggctacccca ggcaacgatg ttaaaccact cggtctctgt acgtcgttcg ccttcagaag
     2641 tgttccacgt gcggctggta gccaagctga aggtggtcac aggtctgcca gacggcgtat
     2701 aacgcatctc tggatcccga cccaagcgac caataagcat cactttattc aagcctcggc
     2761 tcattttcta ctccttcaag tgacacctac acaactactt actttaaagt caccagatag
     2821 cgcaggatgg gttcaagaaa acggacattg cgctcaagct ccgcactgta atcaggggac
     2881 attaccgtgt ctagcaacac gtactggccc tctgtttgct tgcggatggg gtaagccaat
     2941 ttgcgcttgc cccacaagtc cactttcaca acctgcccac cggctccagt gatccaaccc
     3001 ttgatcttat cgaccagctc tttaaatgcg ttctcgtcca gatcggggtg gataatgaag
     3061 atgtattcat actcgcgcat gcaggggatg cctcctttcc acgggtttgc ccctaaactc
     3121 ggctgggagc cggtcaggag cggaaagtaa gccacgcatc tgcgtggctc ctgtcaatag
     3181 tataacccaa aggg
//
LOCUS       DBNHOH010000456         1988 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1052637, whole genome shotgun sequence.
ACCESSION   DBNHOH010000456 DBNHOH010000000
VERSION     DBNHOH010000456.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1052637"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="AC16V7_07965"
     CDS             <1..228
                     /locus_tag="AC16V7_07965"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP carboxykinase; PEP carboxylase; PEPCK;
                     catalyzes the phosphorylation and decarboxylation of
                     oxaloacetate to form phosphoenolpyruvate using ATP;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HIR6257094.1"
                     /translation="GELDAVAYAVDPVFGFQVPKSCPGIPDGVMNPATSWPSQEAYMK
                     RYRGLAARFIDNFKKYVEKIPGEVLAAGPKI"
     gene            complement(256..468)
                     /locus_tag="AC16V7_07970"
     CDS             complement(256..468)
                     /locus_tag="AC16V7_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257095.1"
                     /translation="MWRSINKFLDKAAEFLAHRKGLLPVLGIILVLANSVLRFMPSGG
                     WLAETDFMLHLGVILGFIGILLAWAL"
     gene            complement(477..1805)
                     /gene="hflX"
                     /locus_tag="AC16V7_07975"
     CDS             complement(477..1805)
                     /gene="hflX"
                     /locus_tag="AC16V7_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HIR6257096.1"
                     /translation="MAKKIPEPTQRPRERAFMVGVEIRNQDNLLVLQDSLAELELLAG
                     TAGLNVVGQATQRLDHPNPQTFIGPGKVEEVHALVEEVRADVVLFDEELSPRHLRELE
                     KIFGENARIVDRTALILDIFAQHAATREGALQVELAQYEYRLPRLTRAWTHLARQAGG
                     GGGRSGSVGGVGLRGPGETQLEVDRRDIRRRIAHLKVELEKVRTHRQQYRLRRKRSRI
                     PVVALVGYTNAGKSTLLNHLSEADVYVADQLFATLDPTTRRVELPSGNMILVTDTVGF
                     IQKLPTELIAAFRATLEEIVEADLLMHVVDVTHANAKAQAEAVYQTLSEIEADHIPTL
                     SVLNKIDLLADPENARLALANFPNSVAISALTGEGIQDLLGRVNEHLYETYSPVIVRL
                     PYQEGSLIAAFHDQGQVERVEQTHQGVLIQGRLPGRLLARYQPFLVQRAG"
BASE COUNT          470 a          542 c          500 g          476 t
ORIGIN      
        1 ggcgagctgg acgcggttgc gtatgctgtc gatccggttt ttggcttcca ggtgcccaag
       61 agttgccctg gcatccccga tggtgtgatg aacccggcaa cctcctggcc cagccaagaa
      121 gcatatatga agcgctaccg cggtctggca gcccgcttta tcgataattt caagaaatat
      181 gtagagaaaa tcccaggaga agtccttgcc gctggtccaa agatctgatc cgatcgctat
      241 cgataagcga gaagtttaca aagcccatgc cagcaagata ccaataaacc ccaggatcac
      301 ccccaagtgg agcatgaaat cggtctcagc cagccagccg ccactgggca tgaaacgcaa
      361 cacgctgttc gcaagtacca agatgatccc taataccggc aaaagccctt tgcggtgagc
      421 cagaaattcc gcagctttat cgagaaattt attaatgcta cgccacatac gctaaattat
      481 cctgcgcgct gcacgaggaa cggctggtag cgtgccagca gccggcctgg caagcgtccc
      541 tggattaata cgccctggtg ggtttgctca acgcgctcca cctgcccttg atcgtgaaaa
      601 gcggcaatca aactaccctc ttgataaggc aggcgcacta tcacaggaga gtaggtttca
      661 tataggtgtt cattaacgcg acccaatagg tcttgaattc cttccccggt caaagccgag
      721 atagccaccg aatttggaaa attggcaagt gccaggcggg cgttctctgg gtcagccaat
      781 agatcgatct tgttcagcac gctcaaagtt ggtatatgat ctgcctcaat ctcactcaaa
      841 gtttggtaaa cggcttctgc ctgtgccttg gcgttggcgt gggttacgtc aaccacatgc
      901 attaataaat ccgcttcgac gatttcttct aatgtggcgc gaaacgctgc aatgagctcg
      961 gtgggcagct tctggataaa tccaaccgta tcagtcacta gaatcatatt tccgctcgga
     1021 agctccactc tacgggttgt cgggtcaagt gtggcaaaga gttgatccgc cacatagaca
     1081 tcggcttcag acaaatggtt tagcagcgtg gattttccgg cattggtgta gccaaccagc
     1141 gccacgacgg ggatacgcga gcgcttacgc cgtaagcggt attgctgtct atgggtccgc
     1201 accttctcca attctacttt gagatgagcg atacgcctgc gaatatcccg ccggtcaact
     1261 tcgagctggg tttcgccagg accgcgcaac cctacgccgc ccacgctccc agatcggcca
     1321 ccaccaccac ccgcttggcg cgcaaggtga gtccaggctc tcgtcaacct gggaagccgg
     1381 tattcatact gcgccagttc tacctgtagc gcaccctctc tggtggctgc atgctgggca
     1441 aagatatcca atatgagggc agtacggtca acgatgcggg cattttcgcc gaagattttc
     1501 tcaagctcac ggagatggcg cggcgagagt tcttcgtcga atagtacaac atccgctcgc
     1561 acctcttcga ccagagcatg tacttcttcc acctttcctg gcccgatgaa agtttgagga
     1621 ttgggatgat ccaagcgttg agttgcctgc ccaacgacat ttaatccagc tgttcccgct
     1681 agaagttcaa gctccgcaag agaatcttgc aatactagca gattgtcttg atttcggatt
     1741 tcaacaccca ccataaaggc acgttcgcgg ggtcgctgag taggttcagg aattttcttc
     1801 gccatattac ttataaggat ccttcaactc gccagtctga attgatgacc ggaatagctt
     1861 ggccattttt tcatctggag gtcgtttacg gattgggagc agtatgtgga atttggaacc
     1921 tgggcatcct acgtcatcat gtgcttctga ttcgacccat attgttccgc catgagcttc
     1981 aaggatgc
//
LOCUS       DBNHOH010000457         2199 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_282533, whole genome shotgun sequence.
ACCESSION   DBNHOH010000457 DBNHOH010000000
VERSION     DBNHOH010000457.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_282533"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="AC16V7_07980"
     CDS             <1..377
                     /locus_tag="AC16V7_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6257097.1"
                     /translation="TGRKVEIPFDVLVIFSTNLPPKDLVDEAFLRSLRHKIEIGDPNY
                     NDYREIFRRVAVAKDITYSDRGLAYLLQEWYIKRGRKMRASHPRDLCDQILDIARYLS
                     VEPAMTRDLLDRAAESYFVELY"
     gene            389..1168
                     /locus_tag="AC16V7_07985"
     CDS             389..1168
                     /locus_tag="AC16V7_07985"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015000.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIR6257098.1"
                     /translation="MLDQLPNPAIIAHRGASAYAPENTLAAFELAVRQKADAIELDAK
                     LTLDNHVVVFHDQTLERTTGVHGRVIGSKLADLRRLDAGSHFDVAYRGETIPTLDEVF
                     ELVGRNILINVELTNYASPTDHLPEQVAEIVQKHNLAQRVMFSSFNPIALLRIHRKLP
                     QVPIGLLAYDGRKGSWARSSLGELIRYQSLNISLEDAEASLVDRVHRKGRRLLVYTVN
                     QAEDMRRLFQLGVDGIFTDDPLTAKRVLSTGSEQRLKNKES"
     gene            1165..2124
                     /locus_tag="AC16V7_07990"
     CDS             1165..2124
                     /locus_tag="AC16V7_07990"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HIR6257099.1"
                     /translation="MIPYTWIEQARARIAAHILHTPVTFDPELNTYLKWENLQVTGSF
                     KARGAFNKVFTLQAWELERGLVAASAGNHGQGVALAGSKAGAQVLVFASEHAVPAKLD
                     AMRALGAELRLIPGGYSEAERAGLEYSANSGTTWVSPYTDGQVIAGQGTLVLETLEDM
                     PELASATWVVPVSGGGLISGVGAALKEGSAPPQAKLVGVQTEASPFMYGLYHNGTQEN
                     AVELPSLADGLAGPVEEGSITIPLVRQYVDDIVLVNESEITNAIHYAWEKYHERIEGS
                     AAVSLAAILNNRVIDRPTVLVLSGGNIQPKVHQEIVGGEAWKT"
BASE COUNT          549 a          554 c          592 g          504 t
ORIGIN      
        1 acacgggtcg caaggtcgaa ataccctttg atgtactcgt gattttttca accaacctgc
       61 ccccgaaaga cctggtagac gaagccttcc tacgtagctt gcggcataaa attgaaattg
      121 gtgacccaaa ctacaacgat tatcgggaga tcttccggag agtggcagta gccaaggaca
      181 ttacctatag tgatcgcggg ctggcttact tgcttcaaga gtggtatatc aagcgagggc
      241 gtaagatgcg cgcctcccat ccgcgtgacc tgtgcgatca aatcctggat atcgcccgtt
      301 atttatcggt tgaaccggcg atgacacgcg atcttctcga ccgggcggca gaatcgtatt
      361 ttgttgaact gtattaaaaa ttcaacctat gttggaccaa cttcccaatc ctgccattat
      421 cgctcacagg ggcgctagcg cctacgcgcc tgaaaatacg cttgcagcgt tcgagctagc
      481 tgtgcgccaa aaggctgatg cgattgaact cgatgccaaa ctcacgctcg acaaccatgt
      541 ggtagtattt catgaccaaa ccctggagcg aacgaccggc gttcatggac gggttattgg
      601 ctccaaatta gctgatttac gccgcctaga tgcaggcagc cacttcgatg tagcttatag
      661 gggtgagacg atcccgacct tggacgaggt tttcgagctg gtgggcagga atatcttaat
      721 caacgttgaa ctgaccaact acgcctctcc caccgatcac ctgcctgagc aagtcgccga
      781 gatcgtgcag aaacacaacc tcgcacagcg ggtgatgttc tcatctttca atcccattgc
      841 cctgctacgc atccatcgca aacttccgca ggtacccatc gggttattag cttatgatgg
      901 gcgcaagggc tcctgggcac gctcctcgct aggagagcta attcgctacc agtcgctgaa
      961 tatctccctt gaagacgctg aagctagcct ggtagaccga gttcaccgga aaggtcgccg
     1021 cctattggta tacactgtca atcaagctga agatatgcgc cgcctcttcc agcttggggt
     1081 ggacggtatc ttcaccgacg accctctaac tgcgaagagg gttctatcca caggcagcga
     1141 gcaacgcctg aaaaacaagg aatcatgatc ccatacacct ggattgagca agcgcgggcg
     1201 cgtatcgctg cgcacatttt acacacacca gtcacatttg atccagaatt aaacacctac
     1261 ctgaagtggg aaaatctgca ggtcacaggt tcattcaagg cgcgtggggc attcaacaaa
     1321 gtgttcaccc tgcaagcttg ggaattagaa cggggactgg tagcggcttc ggctgggaat
     1381 catggacaag gggtcgcttt ggctggttcc aaggcaggtg cccaagtact agtctttgcc
     1441 tcagaacatg ccgtgccagc caagctggac gctatgcgag ccttaggtgc ggaattgcgg
     1501 ctaattcccg gcggatacag tgaggctgaa agagcagggt tggaatattc tgcgaatagc
     1561 gggacaacct gggtttcgcc ctatactgac gggcaggtaa tcgccgggca aggtacgctg
     1621 gtgcttgaaa cgctggaaga tatgccagaa ctggcgagcg ccacttgggt ggtgcctgtc
     1681 agcggtggtg gattaatctc tggggtgggt gctgcgctca aagagggaag tgcacctcct
     1741 caagctaagc tggttggagt tcaaaccgaa gcctcgccat ttatgtatgg tctttatcac
     1801 aatggcactc aagagaatgc tgttgaactg cccagcttag ccgatggatt ggcgggtccc
     1861 gttgaggaag gctctattac cattccatta gtacgacaat atgtggacga tatagtcttg
     1921 gtaaatgaat cggaaatcac caacgctatc cattacgcct gggaaaagta ccatgaacgt
     1981 atagaaggat cggcagccgt aagtctggca gctatcctca ataatagagt gatagatcgc
     2041 cctacggttt tagttctatc tggcggaaac atccagccta aagtgcacca agaaattgtg
     2101 ggaggcgagg cgtggaagac ataaccccag atgaggtcat ggtggtatgt attgctcgcc
     2161 aaattcgaga tggcgaaact gttgcccagg gtttggcaa
//
LOCUS       DBNHOH010000458         3046 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_218575, whole genome shotgun sequence.
ACCESSION   DBNHOH010000458 DBNHOH010000000
VERSION     DBNHOH010000458.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_218575"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="AC16V7_07995"
     CDS             complement(<1..658)
                     /locus_tag="AC16V7_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HIR6257100.1"
                     /translation="MKIDSPSSAEVAIIGAGPIGLELAAVLKRLGVDYLHLEARQIGY
                     TISWWPRQTYFFSTTERIEIAGIPLQNTAQQRATGEEYLAYLRSVVEQLDLQVNTYEP
                     VVGIQRTENGFQLITLPQTGERRYLCRKLVLAIGDMHAPNRLGIPGEDLPHVSHYFDD
                     PHKYFRKRLLIVGGRNSAAEAALRCWRAGSQVSISYRREDFDPHVVKHWILPDIKTQI
                     E"
     gene            complement(725..1678)
                     /locus_tag="AC16V7_08000"
     CDS             complement(725..1678)
                     /locus_tag="AC16V7_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HIR6257101.1"
                     /translation="MKKFITIVAVFLISLAYGCQVNPVPTPQVGEAGLIVLAAESFLA
                     DIAQNVAGERLTVETLLPAGLDPHAFEPTPKDVARIADSDILIVNGAGLEEWLENITE
                     AAGGERLVIEASAGLTPRHRSSGGACGASSQIAEGGNNGEGDPHFWLDPSLVIYYVEN
                     IRDGLSQVDPQGEDQYTLNAEAYTARVMELDSWITSQVDQIPSDRRLLVTNHESLGYF
                     ADRYGFQLTGAILPSFSTAASPSAADLARLADCIRSTGAPAIFLETGANPQLAEQLAQ
                     ETGIRVVTELYSHSITPPNGNAPDYLSMMKYNTQTIVNALK"
     gene            complement(1876..2928)
                     /locus_tag="AC16V7_08005"
     CDS             complement(1876..2928)
                     /locus_tag="AC16V7_08005"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
                     /protein_id="HIR6257102.1"
                     /translation="MEALIREWDGETAIIHYDRPTGAWIFIAIHSTRLGPAVGGTRMK
                     PYASVQAALQDAMRLAAGMTYKFATPGMPFGGGKAVIVIPADFEAQSRPALLRRYGAL
                     IHQLGGLFSTGPDVGTSPADMDIIAETGAPYIFARTPAAGGAGDPGPFTARGVFAGIE
                     ETCEHLFGDMSLVGRRMLVQGAGDVGAKLINYLCDAGATVAYSEVDEGAIRKVRDDLG
                     LEFVPPEEVYTTECDIFAPCALGGVLNADTIPQLKCRAVVGGANNQFATQEDAERLRA
                     RGILYAPDYVVNVGGAMAITSMEMKGWSQERAEREVVESVRRALRQIFEVASKEGITT
                     DAAARQLAENRLSAGK"
BASE COUNT          699 a          865 c          788 g          694 t
ORIGIN      
        1 gctcgatctg ggtttttatg tccggcaaga tccagtgttt gactacatgc gggtcgaaat
       61 cctcccggcg atagcttatg ctgacctggc tgcctgctcg ccagcagcgt agggctgctt
      121 ctgcagctga gttgcgccct ccgactataa gcaaacgctt gcgaaagtac ttatgagggt
      181 catcaaagta atgggagaca tgaggcaggt cttcaccggg aatccccagg cgatttggag
      241 catgcatatc gccaatagcc aggaccagct tgcggcataa ataacgccgc tcgcccgtct
      301 gcggtaaagt tatcaattgg aagccattct ctgtccgctg gatgcccacc actggctcat
      361 aggtatttac ttgcaagtct agctgctcta ccacgctgcg caggtatgct aaatattctt
      421 caccagtagc gcgctgctgg gcagtgtttt gcagggggat gccggcgatc tcgatgcgct
      481 ctgtggtgct gaagaaatac gtctggcgcg gccaccagga gatggtgtag ccgatctggc
      541 gggcttccag gtgcaggtaa tccacgccga ggcgcttcaa gactgccgcc agttccaacc
      601 caattggccc ggcaccgatg atagctacct cagcagaact cggcgagtcg attttcatac
      661 gatcccgaaa tcctccctct ataccccaaa ggatttgaaa accttgggga gtcttggcat
      721 tcacttattt tagggcgttg acaatggttt gcgtattgta cttcatcatc gagagataat
      781 ctggggcatt tccatttggc ggggtgatcg aatggctgta aagctcggtg acaacccgta
      841 ttcccgtctc ctgggcgagc tgttcagcta gctgtgggtt cgcacccgtc tccaagaaga
      901 tggctggcgc accggtcgag cgtatacaat ctgccaggcg cgctagatcc gcagcggatg
      961 gagatgccgc agtgctgaag ctgggcaaaa tagcaccagt aagctgaaaa ccatagcgat
     1021 cggcaaagta gcccaaggat tcgtggttgg tcaccagcaa gcgacgatct gacgggattt
     1081 ggtcaacttg cgaggtaatc caggaatcca gctccataac tcgggctgtg taggcttccg
     1141 cgttcaaagt gtattggtct tccccttggg gatcgacctg gctcaatcca tcgcggatgt
     1201 tttccacgta ataaatgacc aggcttggat ctaaccaaaa gtgtgggtca ccctcgccgt
     1261 tatttccacc ttcagcaatt tgggatgaag cgccgcatgc ccccccagaa gatcgatgcc
     1321 tgggagttaa accggctgaa gcttcgatga ccaatcgctc acctcccgcg gcctccgtaa
     1381 tattctccag ccactcctcc agcccagccc cattcacgat caatatgtcg ctgtcagcga
     1441 tgcgagctac atccttgggt gtcggctcga aggcatgtgg atcaagcccc gctggaagca
     1501 atgtctcaac ggtgaggcgt tcgccagcca cgttttgggc aatatcagcc aggaaggact
     1561 cggctgccag tacaatcaag cctgcctccc caacctgagg agtcgggact ggattcacct
     1621 ggcagccata ggcaaggctg ataaggaata ctgcaacgat cgtgataaat ttcttcatca
     1681 aataggctgg gagatttgag gtgtgccacg catccctata attatatagc atgggcggca
     1741 ttcgatggct ggatcaccag gccaaaaacc tgttccagtg acccagatat ggaaaagctg
     1801 aacaagttga cactcggtgg ttatatattg caagcaatcc tgtgaacaat cacctcttca
     1861 tagattttat cgagactact tcccagcaga aagccgattt tcagcaagtt ggcgcgccgc
     1921 ggcatccgtc gtaataccct ctttcgacgc cacctcaaaa atctgtcgca aggcgcgccg
     1981 gacactctca accacctccc gctctgcccg ttcttgcgac caacctttca tttccatgct
     2041 cgtaatagcc attgcgccgc cgacgtttac cacatagtca ggtgcgtaaa gaatacccct
     2101 agcccgaagg cgctccgcat cttcctgggt ggcaaactgg ttgtttgcac ccccaaccac
     2161 tgcccggcat ttcaactggg gaattgtgtc ggcgttgagc acaccaccca gtgcacacgg
     2221 ggcaaaaatg tcgcattctg ttgtgtaaac ttcctcagga ggcacaaact ctaatccgag
     2281 atcgtcgcgc accttgcgga tcgcgccttc gtccacctcg ctatacgcca ctgtagcacc
     2341 ggcatcgcag aggtaattga tcagttttgc cccaacgtca cccgcccctt gtaccagcat
     2401 tcgccggccg actagggaca tgtctccgaa gagatgctca catgtctcct ctattccagc
     2461 gaataccccg cgggctgtga aaggcccggg gtccccagct ccgcccgcgg ctggcgtgcg
     2521 ggcgaatatg tagggagcgc cagtctcagc aatgatatcc atatcagctg gcgacgtgcc
     2581 cacgtctggt ccggtggaga acagaccacc gagctggtga atcagcgcac catagcgtcg
     2641 caatagcgcc ggacgagact gggcctcaaa atcggcgggg attacgatga cagcctttcc
     2701 acctccaaat ggcatgccag gcgtggcgaa cttgtaggtc atccctgcgg caagtcgcat
     2761 tgcgtcctgc aaagctgcct gcacggaggc atagggcttc atccgcgtgc cacctaccgc
     2821 aggcccaagg cgggtggaat ggatggcgat aaaaatccag gctccagttg gtcggtcgta
     2881 atgaataatg gcagtctcac catcccattc cctaatcaaa gcctccatga taagtttgcc
     2941 tcctttcttg tcgcatcaat ttttgagtaa tggcccttga caaaatgaca cctacaggtt
     3001 atacaatgag tattatacgt tacattatgt gtaatacaag ttacca
//
LOCUS       DBNHOH010000459         9956 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_860189, whole genome shotgun sequence.
ACCESSION   DBNHOH010000459 DBNHOH010000000
VERSION     DBNHOH010000459.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9956)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9956)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9956
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_860189"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="AC16V7_08010"
     CDS             complement(<1..653)
                     /locus_tag="AC16V7_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005371124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HIR6257103.1"
                     /translation="MSSNTIKAPVGGETITIRDGLLQVPDHPIIPFVEGDGTGRDIWR
                     ASQRVFDAAVQKAYRGRRKIHWMEVYAGQKAFDKFGSWLPEETVEAFREYLVGIKGPL
                     TTPIGGGIRSLNVALRKALDLYVCQRPVRYFNGVPSPVKHPEYVDMVVFRENTEDIYT
                     GIEFEDGGEDNQRFKRLLKEHFPEDYAKIRFPDTAGIGLKPISKEGTARLVRAAIRWA
                     "
     gene            1353..4235
                     /locus_tag="AC16V7_08015"
     CDS             1353..4235
                     /locus_tag="AC16V7_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012996064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR6257104.1"
                     /translation="MTIEMIPDQDKITDLNDGNSYVLQSEEVIRRLATFSDKGLSSEE
                     AERRLKEFGPNALTEAPPPTFWQMLWEQFNNFVVIMLIVAALISGILGEFIEAAAIMA
                     IVVLNAALGVVQQRRAEQALAALRKLASPEAHVIRDGSRQVIPSPQLVPGDLVLLEAG
                     NYIPADIRLLEAVNLRIEEAALTGESVPVQKDATARLESGIPLGDRKNTAFMGTLVNY
                     GRGRGVVTDTGMRTQIGLIAEMLQSVQQEPTPLQRRLDQLGKTLGWGALTICALVFVV
                     GWARGFNPLDMFMIAVGLAIAAVPEGLPAVVTISLALGMREMIKRHALIRRLSSVETL
                     GSATTICSDKTGTLTQNEMTATRMWVDGKFIEITGSGYNPNGDFIIDGKAVNPTDYQG
                     ALTALWVATLNNDADLEITGASDGRQTYRMVGDPTEGALIVAAAKVGARTRELNEAYP
                     RVQEVPFDATRKRMVTVHALRHPKPEDISPFYDEKPHGYVVAVKGAPDIVLDLCSSYQ
                     HMGDYPTPLDESQRMRILSANDSLTQDALRVLGLAYKVIPEQPDIEDMQALEEDLIFV
                     GLIGMIDPPRLEVPPALDVADQAGIRTIMITGDYPNTARAIAESIGLLRPGHQVLTGS
                     EMNQMDDQTLQRQVEYTDVFARVSPEHKLRIVEALQANGEVVAMTGDGVNDAPSIKKA
                     DIGIAMGITGTDVAKESADMVLTDDNYASIVSAVEQGRIIYANIRKFVFFLLSSNVAE
                     IMIIFLATLAGLPTPLTVIQLLWLNLLTDGAPALALAMEKGDPDEMNRPPRPPSEPII
                     NKSMQLGIFIQTITQTGAVLTAFGLGLLWHLAQGDILPPGANPLTFLLGYDWRGLDVQ
                     TAETMAFATLSLCELFRAYTVRSERISIFRMGVFSNRYMQYAVGFSIALLLLVVNVPF
                     LQPIFNTHFLKPVEWAVVVGLALIPAISEEITKAALRWRDQRERGE"
     gene            4709..5638
                     /locus_tag="AC16V7_08020"
     CDS             4709..5638
                     /locus_tag="AC16V7_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HIR6257105.1"
                     /translation="MVAIYKNTEQGLSMLTEPTTGCWISAVNPTLDEIHWLQSLGIPQ
                     DYITYPLDVDERSRTERENGELLILLRIPYFQGHSADIPYTTIPLGIILNDRYLVTVC
                     KRETDILNEFADGRVKSLATSKRLRFVLRLLLSTATKYLVYLREINRTVEALEDQLQL
                     STRNKEVLELLKYQKSLTYFTTALKSNELMLERLQRSQIFKAFPEDEDLLEDVITENQ
                     QAIEMTNISSNILSGMMDAFASIISNNLNAVMKFLAAITVVLSLPTMIASFYGMNVDL
                     PLDHLPVAYIYPIAFSLIISIVVVVVFYRRDWL"
     gene            complement(5866..7803)
                     /locus_tag="AC16V7_08025"
     CDS             complement(5866..7803)
                     /locus_tag="AC16V7_08025"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6257106.1"
                     /translation="MPKEQILFFLLDTYIGQLVEDTPLRSAGYEVNLVKDFPAYKALV
                     RASLPDVVILGERLPGEQEDEEINGLDVATSLLEKHPNLPILLVANQPTDALLAEAMR
                     IGMVDCLRPPLRGDDLLLAVQRALQRGQKIKQWARGEVKRNTQSLQARLSSLETLQKI
                     GRNVTSSLDLDTILTAVVDSAVDLTGAEKGSLLLLDEATGELYMRASRNFGEDFARTF
                     RVPVLDTLAGEVLRTGKPVILDEKTPKKIKTAYLVHSLLYVPLVIEESTIGVLGVDNG
                     QSGHPFTEYHLGLVSALAAYAAVAIQNASLYARSEVERNKLEAILTRITDSVIVVGLD
                     GRLVLINQTARHAFAYKPEESLTGKPADEVIHSLDLVEILCDAEATTPFRSEIGLDDG
                     RVFNAQVTPIPEVGVAVILQDITHLKELDRIKSEFVNTVSHDLRSPLTAILGYIELIE
                     RVGPINQQQLEFIQRVEQSVQNITALINDLLDLGRIEAGFDARKEIVPLPPLVDLTID
                     SLLPRFEEKPQHLVVDIPKDLPAVLGNPIRLRQMLVNLVTNALKYTQQGGEIRVSAHA
                     AGGQVILQVSDDGVGIPRADQPYIFDKFYRASNVVREASGTGLGLAIVKSIVENHQGR
                     IWVKSSPGKGTMFTVVLPAAR"
     gene            complement(7824..9026)
                     /locus_tag="AC16V7_08030"
     CDS             complement(7824..9026)
                     /locus_tag="AC16V7_08030"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6257107.1"
                     /translation="MGNQQEKILLVESSHKNSDLIARQILEPLGYRVNVARSAPDALQ
                     EVALFSPDLIITNLKLSGLSGKDLLVALTSQGLEYPVIVLCEKGAEADLIQTFRLGAV
                     DILLWPAREVEVISAVERALNQVRARRDREALARQLQQTNQELQRRVRELTTIFAIGK
                     AVTAITDYHILFERIVEGAMYICEADSAWLLLRDEQSKAFLLRAQRNLPVSVSAKIGQ
                     PWDDGISSLVAASGEPLIIHGEPLRRFKISLLGRSAMVLPVKVNNEVVGLLAVLRKSA
                     VPFGANSQALLSAVVDYASISIVNANLFKALEERTHRLQQAANADQDEERNKDELLNR
                     LRQEMSAPLAEADQAIESLLIDEQSGLGDSQKSLLRQTQECLHRMAELLTSQPSNAKV
                     KDGLGTLN"
     gene            9261..>9956
                     /locus_tag="AC16V7_08035"
     CDS             9261..>9956
                     /locus_tag="AC16V7_08035"
                     /inference="COORDINATES: protein motif:HMM:NF013705.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HIR6257108.1"
                     /translation="MEYKDYYKTLGVDRKAKEDEIKRAYRKLALKHHPDRNPNDKKAE
                     EKFKEINEAYQVLSDPQKRTRYDQLGDSYHHWQQRGGTPGGFNWDEWVTTPNQSNVRV
                     EVGNLEDLFGGGLGDFSDFFRSVFGGMSGMGTQRSSRSRAAERTRIPSSQSEVTISLR
                     EAFQGTTRRIEVDGRHLEVKIPRGARTGTKIRVAQALTSPNGSKGDLYLVIKVADEPN
                     FQSKGNDLYTDVEV"
BASE COUNT         2362 a         2732 c         2643 g         2219 t
ORIGIN      
        1 gcccagcgga tggccgctcg taccaagcga gccgtcccct ctttggagat cggcttcagg
       61 ccgatcccgg ctgtatcagg gaagcggatt ttggcgtaat cttccggaaa atgctctttc
      121 aataatcgct tgaagcgctg gttatcttcc cctccatctt caaactcgat gcccgtgtag
      181 atgtcttcgg tgttctcgcg gaagacaacc atgtccacgt attccgggtg cttgaccggg
      241 gaaggcacgc cattgaaata gcgcacgggt cgctggcaaa cgtacaggtc aagggcttta
      301 cgcagggcaa cgttcagaga acggatgcca ccaccgatgg gtgtggtcaa ggggcctttg
      361 atgcccacca aatactcgcg aaaggcctcc acggtttctt caggcagcca agaaccgaat
      421 ttatcgaagg ctttctgacc agcgtagacc tccatccaat ggatcttgcg cctgcctcga
      481 taggctttct gtacggcagc atcaaacacg cgctgcgacg cccgccagat atcgcgtccg
      541 gtgccatctc cttcgacgaa ggggataatg ggatgatccg gcacctgcag caacccatct
      601 cggatggtaa ttgtctctcc gcccactggc gcttttattg tattggaact catgaaatgc
      661 tctcccttgt atttagataa ccgcaagaca attaaaccac aaaggacaca agtacacaaa
      721 gagtattttg aaaatagctt taacaaataa aaccaacgta actacttgcc catcttccct
      781 ttccagtgag aaagggaaga agagagtcaa ggttttcacc tagatggttt ggattgatcg
      841 acattgaaaa actgtgagtt aaaagtgaaa atagcttgcc aaccatgcaa atatttgtta
      901 tgattacttg taggtgggga gtagctgcct gccatctagg taggggaact gtcgtcatca
      961 cggaattttt cccggcagtt caattgcttt cctcgattca gccagaccac catgacgata
     1021 gttgtggtgg tttgtatctt aatagggctc caatgcacag gctttcgtta gatgactttc
     1081 tcaatagagc acaacgcaaa acaaaggaga agctatgtac atgcgatggc taagccagtt
     1141 catgttattg agactggttg atttggtcct cttggtgttt tttctcatcg ccttgatcca
     1201 tcaacccata cgaaagctca tcattcgcct ccagaggtaa agttgggtat gaattttcag
     1261 gttgccatct cggcgcgcgc tagaaataaa gagaattaag gagtaggcga gcaatttgat
     1321 cttccgccaa ccccattcgg atagagtcaa ctatgacaat tgaaatgatt ccagatcaag
     1381 acaaaattac cgatctaaat gacggcaact cttacgtact tcaatccgaa gaggttatcc
     1441 ggcgtttagc aaccttttcg gataaagggc tatcctcgga agaggcagag cgccgtctga
     1501 aagaattcgg ccccaacgca ctcaccgaag ccccgccacc caccttctgg cagatgctgt
     1561 gggagcaatt caacaacttc gtcgtgatca tgctgatcgt ggcggcactc atctcgggca
     1621 tcttgggcga gttcatcgaa gccgcggcga ttatggcaat tgtagtgctg aatgccgccc
     1681 tgggagtcgt ccaacagcgc cgtgctgagc aagctttggc agctctgcgc aagctggctt
     1741 cgcccgaggc gcacgtgatc cgtgatggct cgcgccaggt gatcccatcc cctcaactgg
     1801 ttccaggtga cctggtgctg ctggaagccg gcaactatat ccctgcagat atccgcttgc
     1861 ttgaggcagt aaatctgcgt atcgaggagg ctgccctgac cggcgaatcc gtccccgtcc
     1921 agaaggatgc tactgcccgc cttgagtcgg gtattcccct gggagaccgc aagaacacgg
     1981 ccttcatggg cactctggtc aactatgggc gcgggcgcgg cgtcgtgacg gacacgggta
     2041 tgcgcacgca gattggcctg atcgccgaaa tgctgcaatc ggtgcagcag gaacccacgc
     2101 ctttgcagcg ccggctggac cagctgggga aaaccctggg ctggggcgcc ctgaccatct
     2161 gcgccctggt gtttgtagtg ggctgggcgc gcggctttaa cccgctggat atgttcatga
     2221 tcgcagtagg gctggcgatc gccgcagtgc ccgaaggctt gcccgccgtg gtgaccatca
     2281 gcctggcgct gggcatgcgc gagatgatca agcgccacgc cctaatccgt cgcctgtcgt
     2341 cggtagagac gctgggctcg gcgaccacaa tctgctccga caaaaccggc acgctgaccc
     2401 agaacgagat gaccgccact cgcatgtggg tggatggaaa gtttattgag atcaccggca
     2461 gcggctacaa ccccaacggc gatttcatta tcgatggaaa agcggtcaac cccaccgatt
     2521 accagggggc actgactgct ttgtgggtgg ctacgctcaa caacgacgca gatctcgaaa
     2581 tcaccggtgc cagcgacggc aggcaaacct atcgcatggt gggagatcct actgaaggcg
     2641 cgctgattgt tgcagcagct aaggtcggag cgcgcacgcg agagttgaac gaggcttatc
     2701 cacgcgttca agaagtgccc ttcgacgcca cgcgcaagcg catggtaacc gtgcacgccc
     2761 ttcgtcaccc caagccggaa gatatctctc ccttctatga tgagaagcca catggatacg
     2821 tggtggcagt gaaaggcgcg ccagacattg tgctcgatct atgctcttcc taccagcata
     2881 tgggtgatta ccctacccca ctggacgagt cacaacgcat gcgcatcctg tcggccaatg
     2941 acagcctgac acaggacgcc ctgcgcgtgc taggccttgc ctataaagtc atcccggagc
     3001 agcccgatat cgaagatatg caggccctgg aggaagactt gatattcgtc gggctgatcg
     3061 gcatgatcga tccgccacgc ctcgaggtgc cgccagcgct cgacgtagcc gaccaggcag
     3121 gtatccgcac gatcatgatc accggcgatt accccaatac tgctcgcgcc atcgccgaat
     3181 cgatcggctt gctgcgcccc gggcaccagg tgctcaccgg cagcgagatg aaccagatgg
     3241 acgatcagac acttcagcgc caggtggaat acactgacgt atttgcacgt gtctctccag
     3301 agcacaagct gcgcatcgtg gaagctctcc aggcgaatgg cgaagttgtc gccatgaccg
     3361 gcgacggggt gaatgacgcc ccttcgatca agaaggccga catcggcata gccatgggca
     3421 tcaccggcac agatgttgcc aaggaaagcg ccgacatggt gttgacggat gacaactacg
     3481 ccagcatcgt ctcggcagtg gagcagggac ggatcatcta cgccaacatc cgtaaatttg
     3541 tgttcttctt gctctcatcg aacgtggccg agatcatgat catcttcctg gctaccctgg
     3601 caggattgcc caccccactg actgttatcc aactgctgtg gctgaacctg ctcacagatg
     3661 gcgctccggc cctggcgctg gcgatggaaa aaggcgatcc agatgagatg aaccgcccgc
     3721 caaggccacc cagcgagccg atcatcaaca agagcatgca gctaggcatc ttcatccaga
     3781 ccatcaccca aaccggcgcc gtgctaacag cttttggcct gggcttgttg tggcacctgg
     3841 cccaggggga tatcctgcca ccaggcgcca acccgctgac ctttctgctg ggttacgatt
     3901 ggcgcggatt ggacgtgcaa accgcggaga cgatggcttt cgccaccctc tcgctttgcg
     3961 agctgtttcg cgcctacacg gtgcgttccg agcggatctc gatattccgc atgggcgtgt
     4021 tctccaaccg ctacatgcaa tacgcggtgg ggttctccat cgccctgctg ctgctggtgg
     4081 tgaacgtgcc tttcttgcag ccgatcttca acacccattt cctcaaacca gtcgagtggg
     4141 cggtggtagt cggtctggcg ctgatcccgg cgatctccga ggagatcact aaagccgctc
     4201 tgcgttggag agatcaaagg gagagagggg aatgaatcgc ttgcccccca ggatcacctg
     4261 tgaaagagga atcaaaaggg tactttttcc tgacaatagc atgagcgtca cttgcgccaa
     4321 tatggcgaat gggatatact aacggcaagg catgttatca gattttgttt gcggtttgga
     4381 acttgttgca atgggatgcc cccttatgcg gggacactcc actgcaccca aacgtaccaa
     4441 tttatataag gaggctccgg cttagctagg agttgcgcgc gcacgtccac gcttctcgaa
     4501 acgccggcct cgtctggtct gcgacaaaat ttcgagaagc gttttgtttt attatttgga
     4561 gaagaaagat aaggagtaag acaatgaaag cgaagagagg ggcacgtccc tgccatcgct
     4621 caccatcaca agatatcacc cggggcgagt ccgataagcc gccctgactt taaccctgcg
     4681 ggcagttgcc cggaaaggag agaaacgcat ggtcgctatc tacaagaaca ctgaacaagg
     4741 tctgtccatg ctgaccgagc caaccaccgg ttgctggatc agcgcggtga accccaccct
     4801 cgatgaaata cattggctgc aaagccttgg aatcccacaa gattatatta cctacccttt
     4861 ggatgtcgat gagcgatcgc gcacggagcg ggaaaacggc gagctgctca tcttactgcg
     4921 catcccttac ttccaggggc atagcgccga tataccctat actactatcc ccctgggcat
     4981 tattctgaac gaccgttacc tagtcacggt ctgcaaacgc gaaaccgata tcctgaacga
     5041 gtttgctgat ggccgcgtga aaagcctggc gaccagcaag cgcctgcgct ttgtgctgcg
     5101 cctgctgctc agcactgcca ccaaatacct ggtttacctg cgcgagatta accgaacagt
     5161 agaagctctg gaagaccagc ttcagctctc aacgcgcaac aaagaggttc tggagctgct
     5221 caagtatcaa aaaagcctga cttatttcac cacagcgctc aaatcgaacg agctgatgtt
     5281 ggaacgatta cagcgcagcc aaatcttcaa ggcttttcct gaggacgagg acctgttgga
     5341 agacgtgatc accgagaacc aacaggcgat cgaaatgacc aacatctcca gcaacattct
     5401 cagcggcatg atggatgctt ttgcttcgat catctcgaac aacctgaatg cggtcatgaa
     5461 gttcctcgct gccatcacgg tggtgctcag cctgcccacc atgatcgcca gtttctacgg
     5521 gatgaacgta gatttgccgc tggaccatct acctgtggcg tatatctacc ctattgcgtt
     5581 ctctttgatc atctcgatcg tcgtagtggt cgtcttctac cggcgcgatt ggctgtgagg
     5641 tgcgcttgtg agtaatatca catacaactg atgatctttg acaataactt tggagatgta
     5701 tatttagtat gataagtcta gaaaacggga tttcaggata atctcctgat gttcatcgct
     5761 gtttcctcct ttggcgaaag cctccgccgg gttctcctca ccgccccggc ggaggcgatt
     5821 ttaacgacac atgggctcac gcagttgaag gaaaagtggt tgggcctagc gcgccgccgg
     5881 caggacaacg gtgaacatag tacccttacc tggtgacgac tttacccaga tacgaccctg
     5941 gtggttctca acaatcgatt tcacgatcgc cagccccagg ccagtcccag atgcttccct
     6001 gacgacattg ctcgcccggt agaatttatc aaagatatat ggctgatcag cccgggggat
     6061 acctacccca tcatcactaa cctgtaaaat cacctgcccc cccgccgcat gagctgaaac
     6121 acgaatctca cctccctgct gggtgtattt gagcgcattg gtgaccaggt ttaccagcat
     6181 ctgccgaagc cgaatggggt tccccagcac cgcaggaaga tccttaggta tatctacgac
     6241 aaggtgttgc ggtttttcct caaagcgcgg cagcaagcta tcaattgtga gatccaccaa
     6301 tggcgggaga ggcacgatct ctttgcgggc atcgaaccca gcttcgatcc gacccaggtc
     6361 aagtaaatcg ttgatcagcg cagtgatgtt ctgcacgctt tgctcgacac gctggatgaa
     6421 ctcaagctgt tgctgattaa tcggtcctac cctttcaatt agctcgatgt aacccaggat
     6481 ggcagtcagg ggggagcgca agtcgtggga gacggtgttg acgaactcac tcttgatgcg
     6541 atcaagctcc ttcaagtggg tgatgtcttg caagatgacc gccactccca cttcggggat
     6601 aggagtcacc tgagcgttga agacgcgccc atcgtccagt ccgatttcgc ttcgaaaggg
     6661 tgttgtggct tctgcgtcgc agagaatctc gaccaggtcg agggagtgaa taacttcgtc
     6721 tgccggcttg cccgtcagac tttcttcagg tttataagca aatgcatggc gggctgtctg
     6781 gttgatgagc accaagcgcc catccaagcc aaccacgatc acgctatcgg taatgcgcgt
     6841 caatatggct tcaagtttat tacgctcgac ctcggagcgc gcatacagtg aagcgttctg
     6901 aatagcaacg gcagcgtaag ctgccagcgc tgataccaga ccgagatgat attcagtaaa
     6961 aggatgcccg ctttgtccgt tgtccacacc aagcacaccg atggtgctct cctctattac
     7021 taagggcaca tacaataagc tgtgcaccag gtaagctgtc tttattttct taggggtttt
     7081 ctcgtccaga atcacaggtt tgccagtgcg caagacctca cctgcaagcg tatccaatac
     7141 tgggacacga aaggtgcgcg caaaatcttc accgaagttg cgcgaggcgc gcatatacag
     7201 ttcgccagtc gcctcatcga gcagcaacag gctgcccttc tctgcgccag taagatccac
     7261 cgcagaatcc accacagcag tcaggatggt gtccaaatcg agggatgagg tcacgttgcg
     7321 cccgattttt tgcaaggtct ccaggctgct cagacgcgct tgcagagact gggtattccg
     7381 cttgacctcg ccgcgcgccc attgcttgat cttttgacca cgctgcaagg cgcgctgcac
     7441 tgcaaggagt aagtcatcgc ctcgcaaggg agggcggaga caatcaacca ttccaatccg
     7501 catggcctca gccaggaggg catccgtggg ctgattggca accagcaaaa tgggcaaatt
     7561 gggatgcttc tcaagcaggc tggtggctac atccaacccg ttaatctcct catcttcctg
     7621 ctcaccaggt agtctttctc ccaagatcac aacgtctggc aggcttgctc tcaccaatgc
     7681 cttgtaggct ggaaaatctt tcaccagatt gacttcatac ccagccgatc gcaggggggt
     7741 atcctcgacc aactggccta tatatgtatc taaaaggaaa aaaaggatct gttctttagg
     7801 cattgcgttc gcaacttcct gcttcagttt agtgtcccta gcccatcttt gactttcgca
     7861 ttggagggtt gggatgtcaa tagttcagcc atacggtgga ggcactcctg cgtttggcga
     7921 aggaggcttt tttgtgagtc ccccagtcca ctctgctcgt cgatgagcag tgattcgatg
     7981 gcttgatcag cttcggccag aggcgcgctc atttcctggc gcagcctgtt aagcagttca
     8041 tctttgttcc tttcttcatc ctgatcagca ttggcggctt gctggaggcg gtgggtgcgc
     8101 tcctccagcg ctttgaacag gttagcattg acaatagata tggatgcgta gtctaccacg
     8161 gcactcaata aagcctggct attggcgcca aacggcaccg ccgatttgcg caagacggct
     8221 agcaatccca ccacttcgtt attcaccttg accggcagca ccattgccga cctccccaat
     8281 agcgagatct tgaaacgcct taacggttcg ccgtgaatga tcagtggctc gccagaggca
     8341 gccaccagcg agctaatccc atcgtcccag ggctggccta tcttagcaga gacggatact
     8401 ggcaggttac gctgggcgcg tagaaggaac gctttgctct gctcatcgcg cagcagcagc
     8461 caagcactgt cagcctcgca gatatacatg gcgccttcga cgatgcgctc aaacaggata
     8521 tggtaatctg tgatggctgt aaccgccttg ccaatggcga agatggtggt cagctcacgc
     8581 acccggcgct gaagttcctg gttggtctgt tgcaattgcc gagccagggc ttcccgatcg
     8641 cggcgcgcac gtacttgatt gagagcacgc tcaaccgccg agatgacctc aacttcgcgc
     8701 gccggccaga gcaagatatc caccgcacca aggcgaaaag tttgtatgag atcagcctcc
     8761 gccccctttt cgcacaggac aatcacagga tattcgagac cctgagaagt aagggctacc
     8821 aacaagtcct tgccgctcag accggataat ttcaggttgg tgatgatcag gtcgggggag
     8881 aaaagcgcaa cttcctgaag agcatcaggt gctgatctgg cgacatttac gcgataaccg
     8941 aggggttcca gtatctgcct tgcgatcaga tcactatttt tgtggctgct ctcaacaagc
     9001 agaatctttt cctgctggtt gcccatgaga taatcatagc atggttgagt gaaagatggg
     9061 gacaggaaaa ttgacaattg gctacttgga gggcaaagaa acgttagaat aatataaatg
     9121 ttgccaaaca tacttgactt tgtacgcatg acgcgcatac aatcatagta gtgatgtaga
     9181 agttatgcag tcgatgagca tttcattaca ctgcataagt cctacaatta ttgaagtggt
     9241 ttgaccatca agttggctca atggaatata aagattatta caagactttg ggtgtagacc
     9301 gtaaggcaaa agaagacgag atcaagcgtg catatcgcaa attagcgctg aagcatcacc
     9361 ccgaccgcaa cccaaatgat aaaaaggcag aagaaaaatt taaagagatc aacgaagcat
     9421 accaggtctt aagcgatccg caaaagcgaa ctcgctacga ccaattgggc gattcctacc
     9481 atcactggca gcagagaggg ggaacgccgg ggggcttcaa ctgggacgag tgggtcacca
     9541 cacctaacca aagcaatgtg cgcgtggagg ttggcaacct ggaagatctt tttggtgggg
     9601 gtctgggtga cttttctgac ttcttccgta gtgtattcgg cggtatgtct ggtatgggaa
     9661 ctcagcggtc aagtcgcagc cgggcggcag agcgcaccag gatcccgtcc tctcagtccg
     9721 aggtgaccat cagcctgcgc gaagcattcc aggggaccac gcgccgcatc gaggtagatg
     9781 ggcgccatct agaggtgaaa attccgcgcg gggcgcgcac cggcacaaaa atccgcgtcg
     9841 ctcaggcact cacctcaccc aacgggagca agggcgacct gtaccttgtg atcaaagtag
     9901 ctgatgagcc caacttccaa agcaagggaa acgatctcta cacggatgtg gaggta
//
LOCUS       DBNHOH010000460         3904 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_26736, whole genome shotgun sequence.
ACCESSION   DBNHOH010000460 DBNHOH010000000
VERSION     DBNHOH010000460.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_26736"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="AC16V7_08040"
     CDS             <1..592
                     /locus_tag="AC16V7_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR6257109.1"
                     /translation="NHRQLDYLVIANPADEHIAALPGILERFPPNQVVWAGPTHASSS
                     ARYLQSILSEAGMQPIPTQSGQTLDLGNGAILRVLSANARGAVLLLEWRNFSALLPMG
                     MDTESLQSLPNRLAPVTALLLAESGYAPINPPAWLEHLHPQVVLLSVSPGDHHGLPSP
                     ETLEAVQDYTLLRTDRNGWIELTTDGEQMWVEVEKR"
     gene            complement(721..1941)
                     /locus_tag="AC16V7_08045"
     CDS             complement(721..1941)
                     /locus_tag="AC16V7_08045"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIR6257110.1"
                     /translation="MKLSKRIVLISVLVLSMLLALGPASGAKGLGHISERIYGTTIGG
                     QDVHEYTLTNAGKRPVEVKIITYGGIITSIKVPDKWGNMENVALGFDNLSDYETKNPY
                     FGCITGRYANRIDEGRFTLDGVTYCLDINNPPNSLHGGYVGFDKKVWEVTKAEVGPDG
                     VVLELHYLSLAGEGYEPAECADGEAGYPGNLDVYVTYTLNNKNQIVMDYVATTDAPTV
                     VNLTNHTYWNLAGEGEGDINDHILYLNADRFTPVDPTLIPTGELAPVAGTPFDFTVPK
                     PISDGLRSNAEQVAIGRGFDHNWVLSRPSFDDTSMLLAATLCEPGSRRTLNVWTEEPG
                     IQFYAGNFLDGTLYGTSGRAYRQGDGIALETQHFPDSPNQPGFPSTVLRPGETYATRT
                     IFELLVNKGQCRTK"
     gene            2948..3139
                     /locus_tag="AC16V7_08050"
     CDS             2948..3139
                     /locus_tag="AC16V7_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257111.1"
                     /translation="MKVLVLGGTGLCGKDGAPLLVQLRLGYATDLFIDMTKATMEFGV
                     RLTSHDEYIQKVLVNAPVH"
     gene            3178..3330
                     /locus_tag="AC16V7_08055"
     CDS             3178..3330
                     /locus_tag="AC16V7_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257112.1"
                     /translation="MNKILVRKMSGTVSVVIAREGGVRNWQCWAPYATVSWSLVYAAL
                     GVYWGL"
     gene            3327..3566
                     /locus_tag="AC16V7_08060"
     CDS             3327..3566
                     /locus_tag="AC16V7_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257113.1"
                     /translation="MSRYWFPYDPTLVSDDTTLVVGWLGSVVLLSVSSGDRQSLPTLE
                     TLEVMQGHTLLRTDRNGWIDPTMDGAQLWVEAERR"
     gene            complement(3666..>3904)
                     /locus_tag="AC16V7_08065"
     CDS             complement(3666..>3904)
                     /locus_tag="AC16V7_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257114.1"
                     /translation="EELARIKDELLPVEARLSGPSRLEELARIKDELPPVEARLSGPS
                     RLEELARIKDELLPVEARLSGPSRLEELARIKER"
BASE COUNT          901 a         1009 c         1060 g          934 t
ORIGIN      
        1 aaaccaccgc cagctcgact acctggtcat cgccaacccg gccgatgagc acatcgccgc
       61 cctgccgggc atcctggagc gcttcccgcc aaatcaggtg gtgtgggctg gtccaacgca
      121 cgcctccagc tccgcccgct acctgcaatc catcctctcc gaagcaggca tgcagcccat
      181 ccccacccag agcggtcaaa ccctggacct ggggaatggg gctatcctca gagtgctgag
      241 cgccaacgcc cgcggcgccg tgttgttatt ggaatggcgc aacttctccg ccctgctgcc
      301 catgggcatg gataccgaga gcctgcaatc cttgccaaat cgcctcgccc cggtgaccgc
      361 cctgctgctt gcggaaagcg gttatgctcc gattaacccg cccgcctggc tcgagcacct
      421 gcacccccag gttgttctgc tcagtgtctc ccccggcgac catcatggcc tgccctcccc
      481 tgagacactg gaagctgtac aggattacac tcttttgcgg acggaccgca atggctggat
      541 cgagctaacc acggatgggg agcagatgtg ggtggaggtg gaaaagaggt gattgggcgg
      601 tctgttcgtt gggaacgatt tttggtcaat gatagagcgg gtgcgcattt ccttgtgcac
      661 ccgctccaat ttcagattgc ctggtcgaac gtgactagcg taaatacaga caagctggcg
      721 ctatttggtc ctgcattgtc ccttattaac cagtaattca aaaatggttc tggtcgcgta
      781 agtctcgcca gggcgcagca ccgtggaggg aaaacccggt tggttgggcg agtcgggaaa
      841 gtgctgggtc tccaaagcga tgccgtcgcc ttgccggtag gcgcgaccgc ttgttccgta
      901 gagtgtcccg tccaggaagt ttccggcgta gaactgaatg ccaggctctt ctgtccagac
      961 attcagcgtg cggcgagagc ccggctcgca taaggtagct gccaggagca tggaggtgtc
     1021 atcaaatgag ggccggctca gcacccagtt gtggtcaaag cctctaccga tggcgacctg
     1081 ctcagcgtta gaacgaaggc cgtcagaaat tggtttcggc accgtgaagt cgaacggcgt
     1141 tccagcaacg ggtgcgagtt cgccggtggg gatcagggtg gggtcaaccg gcgtgaagcg
     1201 gtctgcgttg aggtacagga tgtggtcatt gatgtcgccc tccccttctc cagccaggtt
     1261 ccaatacgtg tggttggtca gattaacgac ggtaggtgcg tcggtggttg ccacgtaatc
     1321 catgacgatc tggttcttat tgttcaaggt gtaggtgacg taaacgtcga ggttcccggg
     1381 gtatcccgcc tcgccgtcgg cgcattcggc cggctcatag ccctcgcccg ccaggctgag
     1441 atagtgcaac tcgagaacca ctccgtctgg gccgacctcc gctttcgtca cctcccagac
     1501 cttcttgtca aacccgacgt aaccgccgtg cagggagttg ggagggttat taatgtcaag
     1561 gcagtatgtt acgccgtcga gggtgaacct cccttcatcg atgcggttgg catagcggcc
     1621 tgttatacag ccaaaatagg ggttcttagt ttcataatca gagagattat cgaagccgag
     1681 ggccacattc tccatattac cccatttgtc gggcaccttg atggaggtga tgataccgcc
     1741 ataggtgatg atcttcacct caaccggcct cttgcctgcg ttcgttaaag tatattcgtg
     1801 aacgtcctgg ccaccgatcg tcgttccata aatcctttcg gaaatatggc ctaggccttt
     1861 agcccctgat gctggtccca aggcgagaag catcgagagg acgaggacgc tgattaagac
     1921 gattcgtttt gagagtttca tggtcgttat cctcctatca gttttactat tcggattaaa
     1981 agtctgtttg aattgatccg ctcgatcgcg ttcaagttaa atgaaacgct ctgagctcac
     2041 caaatgtttt aggcaatctg tgaatcgtcg atatagacga gcgataaatg tggaaacttg
     2101 acgattttgc taccatctac ctcgtgtaat atcgagaata tgccaggact atccacaatg
     2161 gagaatgcgg ctatctgatt taatatacag cgaagtgaaa tcacgccgac agaattgatt
     2221 atttaagaaa atagacacct cctcctgcct taacagaatt ggcggtgtcg aagttgtata
     2281 tattgaaatg atggatgatg atttggcgag gttatcctgt caaagcaggt tattgctgaa
     2341 ttaaacaaat aataacagag tcaactttac gagtcaagcg agttttcgtt atatctttgt
     2401 gacaaaatcg gtggatgaat tagaaaagtg gtgaaaaggc aagaaatccc catacctaaa
     2461 ctttgggctt agtaaggaaa caccgcttct agcctggagc agtgaaattt atatccccta
     2521 cgaggtgatg cttatcttcc aagccggaag tatcttttct ggcagaggtg ttgctggggg
     2581 gcatctgttc cttgcgtgag caagccctag tagtgagcta ccgcttgtat cccttatctg
     2641 caggtatatc cctctaaaac gaaataattt tgaacgacat cacttctatc acttggacga
     2701 tagaaaaatc cctggattta tagaataaat tcaggttgcc tctttttgta ctgctgtctc
     2761 ataaccgcta atccccgatc ataccagagc tcccccatag ggcgacattg caatgcagaa
     2821 catatttcaa agaatgacta tattcgccag attagatctc cacccaacct tgccaacagc
     2881 atctctacat acttggttac tagagcatag tatatagaaa gcagagataa cctgaaagag
     2941 ggttcccatg aaagtacttg tcctcggtgg tacgggctta tgtggcaagg atggggcccc
     3001 gctgcttgtg cagttgaggc tgggttacgc tacggacttg ttcatcgaca tgactaaagc
     3061 caccatggag ttcggtgtcc ggctgacttc gcatgatgaa tatatacaga aggtgctagt
     3121 aaatgctccg gtacactgaa gaataaaatg agctagttca taacacaaga aaaaatcatg
     3181 aataaaatcc tcgtgagaaa aatgagcggg acagttagtg tcgtgatcgc tcgtgaagga
     3241 ggggttcgca actggcagtg ctgggcgccg tacgcgacgg tttcgtggtc actggtctac
     3301 gccgcgctgg gggtctactg ggggttgtga gcaggtactg gttcccctac gaccccacgc
     3361 ttgtgtctga cgatacaacg ttggtggtag gctggttggg ctcggtcgtc ctgctcagcg
     3421 tctcttccgg cgatcgccag agcctgccca ccctagagac actggaagtg atgcaaggcc
     3481 ataccctgct gcgcaccgat cgcaatggct ggatcgatcc aaccatggat ggtgcgcagc
     3541 tgtgggtaga agcagagaga cggtaggggc aatcgatcag ggatgttgat accagcctgg
     3601 gctgaccaaa gctcagaaca tctttaccaa ggacgttgag aagtacgatg ctctaattta
     3661 atctatcagc gttccttgat ccgtgccagc tcttccagcc gggatggccc actgaggcga
     3721 gcctcgacag ggagcaattc gtccttgatg cgtgccaact cttccaaccg ggatggcccg
     3781 ctgaggcgag cctcaacggg gggcaattcg tccttgatgc gcgccagctc ttccaaccta
     3841 gacggtccac tgaggcgagc ctcgacgggg agtaattcat ccttgatgcg cgccagctct
     3901 tcca
//
LOCUS       DBNHOH010000461         5498 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1245002, whole genome shotgun sequence.
ACCESSION   DBNHOH010000461 DBNHOH010000000
VERSION     DBNHOH010000461.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5498)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5498)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5498
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1245002"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="AC16V7_08070"
     CDS             <1..578
                     /locus_tag="AC16V7_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257115.1"
                     /translation="IMDWFCNSHFGFGEIDHMLTLSDTTKGITTTQIYSWRVENGLGW
                     GQIKKCVESGTCIDTTSTLSATLTLPNGKPKKNGKCQDLNDEAGALAGETGGSPDTIL
                     GWACDGFNMNEIKKAYEMIHGTGTPAEEILTLRASGLSWGEIKKLLKDAETYSQSNKP
                     NNKPNKPNKPEQGNKSGKTPPGKTKPKPGKP"
     gene            797..2707
                     /locus_tag="AC16V7_08075"
     CDS             797..2707
                     /locus_tag="AC16V7_08075"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase/lipase family protein"
                     /protein_id="HIR6257116.1"
                     /translation="MVLQKLRISLLIGLFSVALSLSHSLPAHSQDSSPEVILLNNRGI
                     PIDHITDGDEIRLRVTLPQSAEQPMQVSFTLVGSETPLGECDIERGQDRCDSLSFNTL
                     GWYWDAASSPVTQRSVQASAGGSTLAVSEPLPVLPRPVVMVHGFSSSWVAWENYLGPD
                     GYLAQIGIQGFAVGDGQFEGTMNTGRLEQPNGSTNTIAQNAAILAEYIASVRKHTGAQ
                     KVDLLAHSMGGLISRYYIDRLMGESEVAQLIMLGSPMAGTDCANLPSALGFYLPAALE
                     IRPNYVQDVFNQQITHRHGIAFHALAGIPILEAIKSPCTAIPSDIAVSRQSVGAIPLH
                     VSEMPILHMELNTSKQVFEEYVRPLLQTTAGNFAYEPDPPLAPPGMEQEQFTRTFTGH
                     VEPGGSQELTIPIDPDVAVASFALFDPTRSLNVTVRGASGNVIELDPVKNGLTIVDNP
                     EALFYLGYGFNNPKPGVWKVTLSTSEKTPAEGADFALSASFQGGARLEAQASQLLPKL
                     DEAVQFSARLDKGGQSLDILQAQTNVRSPDGNQESLSMSQAGEMRQVAWTPTQPGLYS
                     VEISVSGRTPDGMAVERAAYFTVEAQPAPRSPLISWGILCLVMFGILAINLWVYAFLI
                     KKNRQRANPGLS"
     gene            2753..4063
                     /locus_tag="AC16V7_08080"
     CDS             2753..4063
                     /locus_tag="AC16V7_08080"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HIR6257117.1"
                     /translation="MSCSSLRDYIHQLESEGNLTRITAPMSKTYQVSGALKQLEPAAV
                     LFEKVLESPFPVVGNLFSSKAAFASYFGVLPREIIPLLADAIQRRSQPEIIEHAACQE
                     VVVSQPDLDALPILRHCDGDGGNYISSGVVIARHPRYGLNADFHRCMQISKTEMAMRV
                     VKGRHFDAFLGDLGEVEVAVCIGNPPNLMAAAATSVEVGINELEIANALQPLPVTRAK
                     TVDVLVPAEAEFVLEGVVYRDRRHAEGPFVDLTETYDVVRQEPVFVVKTITHRKDAIW
                     QALLPGALEHKLLMGMPREPTIYRKVNEGTRCLDVNVSPGGCSWLHAVVQIDKQNEND
                     GLVAIQAAFDGHRSCKHVFVVDKDIDIYDPQQVEWAMATRFQGDTGLVVREREPGSSL
                     DPSAEAGTHLTTKIGFDLTKPFKTKGKSFEKAAFPQVDLSQFTG"
     gene            4111..5310
                     /locus_tag="AC16V7_08085"
     CDS             4111..5310
                     /locus_tag="AC16V7_08085"
                     /inference="COORDINATES: protein motif:HMM:NF016310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HIR6257118.1"
                     /translation="MKLTDSEQEMLDGVHGRATQKAMQILVALGTIYGAERMLPVSSV
                     QIAGVSYDNLGEAGLQFLTEMADGGGKARVLATLNPAGMDTENWQALGIPEDFARNQE
                     RVIEEFARMGVVTTCTCTPYLSGNLPQFGEHIAWAESSAVCYANSVLGARTNREGGPS
                     ALASALSGITPAYGMHLEQERQPELTIQVEAHVEGTHSFGALGMVMGQKLESTRRKPI
                     PYIVGIEHASLEELKTFCASLATYGSAALFHMAGITPESTRYSPPSTRIKITQADIAA
                     AQRSLNPSTEQDVDFVSLGCPHLSIQEIARLADLLKDKHVTKEFWITTARPTKEIADR
                     IGYTQIIEASGAKFAADTCCVVAPIRGRFQVMATDSAKACYYASGKNQFKTVFKPFDE
                     VVEEALR"
BASE COUNT         1282 a         1572 c         1513 g         1131 t
ORIGIN      
        1 agatcatgga ctggttctgc aactctcact tcggcttcgg cgagatcgac catatgttaa
       61 ccctgagcga taccaccaag ggcattacga ctacacaaat ctacagctgg cgagtcgaga
      121 acggtttagg ctgggggcag atcaaaaagt gtgtagaatc tggcacatgt attgacacga
      181 ccagcacact cagcgctacc ctgacattac cgaacgggaa acctaagaaa aacggcaaat
      241 gccaggatct aaacgatgaa gccggtgcac tggcggggga gaccggcggg tcacccgaca
      301 cgatcctggg ctgggcctgc gatgggttca atatgaacga aatcaaaaag gcttacgaaa
      361 tgatccacgg cacaggcacg ccagcagaag aaatcttaac cttgcgcgct tccggcctga
      421 gctggggtga gataaagaag cttcttaaag acgcggagac atacagccag tctaacaaac
      481 cgaacaacaa gccaaataaa cctaacaagc ctgagcaagg caacaagtca ggcaagaccc
      541 caccgggtaa aacgaaaccc aaacccggga aaccataaga aacctgttgt atctaacacc
      601 ccaaatctaa gatttggggt gttactcaat tgatattccg atcatcccag tccccctcct
      661 ctccgcctac ccccgatggc tgcaggaggg ggatgactgg ttttacctaa cattactggc
      721 ttgacgtact ccgcaaggct ggctatcatt tatcctatgc cgatttattg gccatcaatc
      781 ctttgatagg agccgaatgg tgttacagaa actcagaatt tccctgctta tcggtttgtt
      841 ttctgtggct ctttccctct cgcatagcct gcccgctcac agccaggatt cctcgccgga
      901 agtgatcctg ctaaataatc gagggatacc gatcgaccat atcaccgatg gagatgaaat
      961 ccgcctgcgc gtcacgcttc cccagagcgc cgagcagccc atgcaggtga gctttactct
     1021 ggttgggtcg gagacgcccc tgggagagtg cgatatcgaa cgcggccagg accgttgtga
     1081 cagcctatcc ttcaatactc ttggctggta ttgggatgca gcaagctctc cagtaacgca
     1141 acggtcggtc caggctagcg ctggtgggag cactttggcg gtctcggagc cgttgccggt
     1201 gttgccgcga cccgtggtca tggtgcacgg attctcatcc agctgggtag catgggagaa
     1261 ttacctcggc ccggatggct acctggccca gatcggaatc caggggttcg cggtcggcga
     1321 tgggcagttc gaggggacga tgaacaccgg gcgcctggaa caacccaatg gcagcaccaa
     1381 cactatcgcc caaaatgccg ccatattggc ggagtatatt gccagtgtcc gcaagcacac
     1441 cggtgcgcaa aaggttgacc tgttagccca tagcatgggt gggctgatct cacgctatta
     1501 tattgaccgg ttgatgggtg agagtgaagt cgctcaattg atcatgctgg gctcgccgat
     1561 ggctggcacc gactgcgcca acctgccttc cgcccttggg ttttacctgc cggctgccct
     1621 ggaaatccgc cccaattacg tgcaggatgt cttcaaccag cagatcaccc accgtcacgg
     1681 catcgctttc catgccctgg caggcatccc catcttggag gcgatcaaat caccctgcac
     1741 cgccattcct tctgatattg cggtctcgcg acaaagtgtg ggggcgatcc ccttgcacgt
     1801 ctccgaaatg cccatcctgc acatggagct caacacttct aagcaggtgt ttgaagaata
     1861 cgtcaggcca ctgctgcaaa ccacagcggg taatttcgcc tacgaacctg acccacccct
     1921 ggcgccccct ggcatggagc aggagcaatt cacgcgtacc ttcacaggtc acgtggaacc
     1981 aggtggttcc caggaactga ctatccccat cgatccggat gtagctgtcg ccagctttgc
     2041 gctgtttgat cccacccgct cgcttaacgt gaccgtgcgc ggggcgagcg gcaacgtgat
     2101 cgaactcgac cctgtaaaaa atgggctgac catcgtggac aatccagaag ccctattcta
     2161 cttgggttat ggcttcaaca atccaaaacc cggggtctgg aaggtcacct tgtccaccag
     2221 cgaaaaaacg ccagccgagg gtgcagattt tgcgctcagc gcctccttcc agggaggcgc
     2281 caggcttgaa gcccaggcca gccagctact ccccaagctg gacgaggccg tgcagttcag
     2341 cgcgcgtctt gataaaggcg gtcaatccct ggacatcctg caagcccaga ccaacgtgcg
     2401 ctcccctgat ggcaaccagg aatccctgtc catgagccag gctggggaaa tgcgccaggt
     2461 cgcgtggacg ccaacccagc ctggcctgta ctcggtggag atcagcgtga gcgggcgcac
     2521 gccggatggc atggcagtcg agcgggcagc ctatttcacg gtcgaggccc agcccgcgcc
     2581 gcgctcgccg ctgatttcct gggggatctt gtgcctggtc atgttcggaa tcctggcgat
     2641 caacctgtgg gtgtatgcct ttttgatcaa aaagaacaga cagcgcgcaa accccggtct
     2701 ttcctgagct atcagaaaag agagcatacc tagcactgta aaggactatc ccatgagctg
     2761 ctcatcactg cgagattata tccatcaact ggagtcagaa ggcaatttga cccgtatcac
     2821 cgcccctatg tccaaaacgt accaggtttc cggggcactc aaacagttag aacccgccgc
     2881 ggtgctgttt gagaaggtcc tcgaatcccc cttcccggtg gtgggcaatc tgttctccag
     2941 caaggctgcc tttgccagct acttcggcgt tttacccaga gagattatcc cgctgctggc
     3001 agatgccatt cagcgccgct cccaacccga gataatcgag catgcagcct gccaggaagt
     3061 ggtcgtcagc caacccgacc tggatgctct gcctatcctg cgccactgcg acggggacgg
     3121 cgggaattac atcagctctg gtgtggtaat tgctcgccat ccgcgctacg gcttaaacgc
     3181 tgacttccac cgctgcatgc agatctcaaa aaccgagatg gcaatgcggg tggtgaaagg
     3241 cagacatttc gatgccttcc tgggcgatct gggcgaggtc gaggtagcgg tatgcatcgg
     3301 caaccctccc aatctgatgg cagcagcagc cacctcggta gaggtcggca tcaacgagct
     3361 ggagatcgcc aatgcattgc agcccttgcc agtgacgcgt gccaaaactg tagatgtgct
     3421 tgttccagcg gaagccgagt ttgtcctgga gggcgtggtc taccgcgacc gccgtcacgc
     3481 cgaaggtcca ttcgtcgacc tgaccgagac ctatgatgta gtgcgccagg agccggtgtt
     3541 cgtggtcaag accatcacac atcgtaagga tgcgatctgg caagccttgc tccccggagc
     3601 gctggagcac aagctgctga tgggcatgcc acgcgagccc accatctacc gcaaggtcaa
     3661 cgagggtacc cggtgtttag atgtgaacgt cagccctggc gggtgttcct ggctgcacgc
     3721 agtcgtgcaa atcgacaagc aaaacgaaaa tgacgggctg gtagccatcc aggctgcttt
     3781 tgacggtcac cgctcttgca agcatgtctt cgtggttgat aaggatatcg acatttacga
     3841 tccccagcaa gttgaatggg cgatggcgac gcgctttcag ggagataccg gattggtcgt
     3901 cagggagaga gagcccggct caagcctgga tcccagcgca gaggcaggca cgcatctcac
     3961 cacaaaaatc ggttttgacc tgaccaagcc ctttaaaacc aaggggaaat cctttgagaa
     4021 ggcagctttc ccgcaagtgg accttagcca atttaccggc taacctgcgc atggcttaat
     4081 agtagcagga atgagaaggc tatactcaat atgaaattga cagactcaga acaagaaatg
     4141 ctggacggag tgcatggccg ggcaacccag aaggctatgc aaatccttgt tgcgctaggc
     4201 acgatatatg gcgccgaacg catgctgccg gtgagctcgg tacagatcgc cggagtgagc
     4261 tatgacaacc tgggtgaggc tggactgcaa ttccttaccg agatggctga tggcggtggt
     4321 aaggcacgtg tactcgccac gctcaaccca gctggcatgg acactgaaaa ttggcaagct
     4381 ctaggcatcc cagaggactt cgcccgcaac caggagcgcg tgatcgaaga gttcgctcgt
     4441 atgggagtgg tcaccacctg tacttgcacc ccatacctct cgggcaacct gccccaattt
     4501 ggggagcata tcgcctgggc tgagagcagt gccgtgtgtt atgccaactc cgtcctggga
     4561 gcgcgcacca atcgggaagg agggccaagc gccctggcgt ccgccctcag cggtataaca
     4621 cccgcctacg gcatgcatct ggagcaggag cggcagcctg aattgaccat tcaggtggag
     4681 gcacatgtgg agggtacgca tagctttggc gccttgggga tggtcatggg ccagaaacta
     4741 gaatcgaccc gtcgcaagcc tatcccatac attgtaggta tcgagcatgc atcgctggaa
     4801 gagttgaaaa ctttctgcgc cagcctggcg acctatggca gcgctgcgct tttccatatg
     4861 gccgggatca ccccagaatc gacccgctat tcaccaccat cgacccggat taagataacc
     4921 caggctgata ttgcagccgc ccagcgttca ctgaacccca gcactgagca ggacgtcgac
     4981 ttcgtcagcc tgggctgccc gcacctctca atccaggaga ttgcccgatt ggccgacttg
     5041 ctcaaggata agcatgtaac caaggaattt tggatcacca ctgccagacc gactaaggag
     5101 atcgccgacc gtataggcta tacgcaaatc atcgaggcga gcggagcaaa gtttgctgcc
     5161 gacacttgct gcgtggtagc acccatccgc gggcgtttcc aggttatggc aaccgatagc
     5221 gccaaggcgt gctattatgc ttccgggaag aatcaattca aaactgtctt caagcctttt
     5281 gacgaagtcg ttgaggaggc tttgagataa ttatccacca ttgcgccaat ttctggtatt
     5341 tccctttgcg caattggcat tatttgcgga taaaccccct ggcataaata tgttgataca
     5401 tggaagaaca atctttgcag gggtagccca gggagaggct ctggttactt ccatgggcat
     5461 ctccttttat ggtggcgtcg acccagagag cggtatcg
//
LOCUS       DBNHOH010000462         1903 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1308782, whole genome shotgun sequence.
ACCESSION   DBNHOH010000462 DBNHOH010000000
VERSION     DBNHOH010000462.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1308782"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..893)
                     /locus_tag="AC16V7_08090"
     CDS             complement(153..893)
                     /locus_tag="AC16V7_08090"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HIR6257119.1"
                     /translation="MDFPFYTHPIPYLTKQQMVEVDRAMVEDYQIELAQMMENAGRNL
                     AHMARVRFLGGDPRAKRVVVLAGSGGNGGGALVCARRLHNWGAQVIILITRHDAEFTP
                     TPAHQLGILRHMGVQVEPAESLERASDSELVIDGLIGYSLQGAPRGSAATLIRWANAQ
                     RAPILALDIPSGLDATSGAAYNPTIRATATMTLALPKEGLRAEAAQAYTGELYLADIS
                     VPPGLYSEPSLGLQVEPIFAESEIIRLT"
     gene            complement(1085..>1903)
                     /locus_tag="AC16V7_08095"
     CDS             complement(1085..>1903)
                     /locus_tag="AC16V7_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257120.1"
                     /translation="IAYCTRQILRWFVPLSVIDRLPLWVLPALVLVVLLLFARKRHWA
                     SFFKQLSDSPQWIMITFSAIYLVIIVLTTITGDHLHPHDDRFQSVVFLPVLVLLFTVL
                     QELIIAPLEERKLNLTRPVLVFLILVWSIYPVFTLWKYVQKSVQDGEAIYNLYNLRVY
                     QESPVIRYLKEHPPEPGIPLYSNDSEAVYFFIRKTVELSPTDFVNNQRSEAYLLEHYQ
                     GWPNAEMAYLVWFVERGDRRYYYPPDELEEVAILEPLVESRKVGGVYLVRPLSR"
BASE COUNT          451 a          474 c          558 g          420 t
ORIGIN      
        1 catcgccagc gccagtgtgg agacgcgcgg caggatggtt gatatggcgc tggataactt
       61 gcgggcaggt ctgcatggtg agcgtttgcc caactgcgtt aatcctgaga tatatcgata
      121 gagggcaatg gtggtagtgc ctggttgtcg tgctaagtta gccgaataat ctcactttca
      181 gcaaaaattg gttcgacctg caagcccagg cttggctcgc tatacaaacc tggtggcacg
      241 ctgatatcgg ccaggtacag ttcgccggtg taagcctgag cagcttcagc gcgcaagcct
      301 tctttgggca gggccagggt catggtagcg gttgcgcgga tcgtgggatt ataggctgcg
      361 ccgctggtgg cgtccaaacc cgaggggata tccaaagcca ggatgggggc tcgttgggca
      421 tttgcccatc ggatcagcgt cgcggcggaa ccacgcgggg caccttgcag gctatacccg
      481 atcagtccat cgatgaccag ctcgctatcg ctggctcgtt ctaatgattc ggcgggctct
      541 acctgcacac ccatatgccg caagatgcct aattgatggg ccggagtagg ggtgaattct
      601 gcgtcatggc gggtaatcaa gatgatcacc tgggctcccc aattatgcag cctgcgagcg
      661 cagaccagcg ctccaccacc attgccaccg cttccagcca gaacgacaac tcgcttagcg
      721 cgcggatcgc cacccaagaa gcgaacgcgc gccatgtgtg ccaggttacg tccagcgttc
      781 tccatcatct gtgccagctc aatctggtaa tcttcaacca tggctcgatc gacttcaacc
      841 atctgctgct ttgtaaggta ggggattggg tgggtgtaaa agggaaaatc cattcagtat
      901 tcctccagat tatctgtatt caatgatccc ctagcctgtc tgtgttgcaa cggtatgttc
      961 ggctgcgtgg atggtggtga gggaatagct ccggtctgcc tcgcggaagc gctaccggcc
     1021 aggcgtttgt gttctgcgtt ttatgatccc cgaacacgaa agatgacaaa gtgaagataa
     1081 tttattaccg agagaggggg cgtacgaggt acacgcctcc gactttcctt gattccacca
     1141 gaggctccag aatagccacc tcctccagtt catctggggg ataataatat ctacggtcgc
     1201 cgcgctccac gaaccatacc aaataagcca tctctgcatt aggccatccc tggtagtgct
     1261 cgagcagata agcttcgctt cgctggttgt ttacaaaatc agtaggagat agttcgacag
     1321 ttttgcggat aaaaaagtag acagcttccg aatcattgct gtagagtggg atgccgggct
     1381 cgggcgggtg ctccttgaga tagcgaatta ccggcgattc ctggtaaact cgcaggttgt
     1441 ataggttgta gatcgcctcg ccgtcttgga cggatttttg cacgtatttc cacagggtga
     1501 agacagggta aatagaccag actaagatca ggaataccag cacaggccgg gtaagattga
     1561 gcttgcgctc ttccaggggg gcaatgatca gctcttgcaa aactgtgaaa agcagcacca
     1621 gcacaggaag aaagacaacg gattgaaagc gatcgtcatg gggatggagg tgatcaccag
     1681 tgatggtggt gaggacgatt attaccaaat agatggctga aaaggtgatc atgatccact
     1741 ggggtgaatc ggatagctgc ttgaagaagg atgcccagtg gcgtttgcgg gcaaacagca
     1801 gcaaaaccac cagaacgagc gccgggagga cccagagagg tagccggtcg atgacgctca
     1861 atggcacaaa ccagcgcaag atctggcgcg tgcagtaggc gat
//
LOCUS       DBNHOH010000463         2498 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_989127, whole genome shotgun sequence.
ACCESSION   DBNHOH010000463 DBNHOH010000000
VERSION     DBNHOH010000463.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_989127"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            272..1420
                     /locus_tag="AC16V7_08100"
     CDS             272..1420
                     /locus_tag="AC16V7_08100"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: protein motif:HMM:NF012870.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016893 - obsolete endonuclease activity,
                     active with either ribo- or deoxyribonucleic acids and
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase catalytic domain-containing protein"
                     /protein_id="HIR6257121.1"
                     /translation="MMSLKSKRELLEAVRARYLKASKAEKQTILDEFTCATGYHRKHA
                     IRVFKNQRQIQNHLKGKTKTYKTLYGGEVVQALEQIWEIYGRICSKRLQPILPEAIKV
                     LERCKEIELSKETKELLLKISSASIDRCLRPIRCKSPHGLSTTKPGSLLKTLIPVRTF
                     TEWDQERPGFMEIDLVAHCGNTTEGQYLNTLTCTDISTGWTDVTALAHRSQQVVSTAI
                     QRMRQRLPFPLLGIDSDNGSEFINDTLYRYCLDEKITFTRSRPYKKNDQAHVEQKNWS
                     VVRHTVGYDRWETELELALLESIYDDLRLYINFFQPSFKLISKERIGNRTLKRYDRAK
                     TPYQRVLERKDVSIESKARLMNLYLQLNPAELRRRIDQKTAKLWKISR"
     gene            1588..>2498
                     /locus_tag="AC16V7_08105"
     CDS             1588..>2498
                     /locus_tag="AC16V7_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257122.1"
                     /translation="MECSTRRMEQVAKLLAEEIAEKLAGKQELDEMESMMRELVREAA
                     NVGLRRVIEQGEETYGSREVICPCGQKAQFVSKRSAVLWTVFGKVDYQRRYYLCPTCH
                     QGQCPLDLKYGIVPGQTTPTLASLLGMLGVEVSFAEASRLAERFLLFRVSDNTVRKQT
                     EGYGNAQAQVEQEWIENAEDEKALQVRERSLEPQPGRIYASIDGAHVPLQQEWRELKT
                     LCWYKVEKLRPSIPRKHHGQPMDGQSDLQAKEMKYHCDITEAEQFGRLLWATGLQNQA
                     DTYEEVVFVNDGALWIWRLVEKYFPQAV"
BASE COUNT          725 a          589 c          643 g          541 t
ORIGIN      
        1 cttctcctta cttcgccact cgcaatacgc agggtcactg gtatccagtg gaatgagttt
       61 agcggggatg gataacccgc tcaaggacgc ttttgcccgt cctaggcaag atatgtggcg
      121 cttttcgcgc cacggcggag cattataaca caagataggg gagacgcgcg cttcgcgaat
      181 tgcatcatgg aaaatgttac tatggagggt atcgttgcat caggaaaaat gttacttacc
      241 gataggcagc aaagccagtc ggaggagcaa catgatgagc ctgaaaagca agcgcgaatt
      301 gttagaagca gtgcgagcca gatacctgaa agcgagtaaa gcggaaaagc aaacaatcct
      361 ggatgagttt acttgtgcca ccggatatca ccggaaacat gccatccggg tgttcaaaaa
      421 ccaaaggcaa attcaaaacc acctaaaagg gaaaaccaaa acctacaaaa ccctctatgg
      481 tggtgaagtc gtacaggcac tcgaacaaat ctgggaaatc tatggacgga tctgctccaa
      541 acggctgcaa cccatattac ccgaagcgat caaagtgctg gagcgttgca aagaaattga
      601 actttccaaa gaaaccaaag aactactcct gaaaatcagt tccgccagca tagaccgttg
      661 cttgcgtccc atccgttgta agtcaccaca cggcttgagt accaccaagc ccgggagcct
      721 gctcaaaacc cttattccag tacgcacctt taccgaatgg gaccaagagc gacccgggtt
      781 catggaaatt gacctggtgg cgcattgtgg gaacaccaca gaagggcaat acttgaatac
      841 tttgacctgt accgacattt ctacaggctg gacggatgtc acggctctgg cacaccgctc
      901 gcaacaggta gtttctacag ccattcagcg catgcgccaa agattgccat ttcctttgct
      961 gggcattgat tcggacaatg gcagtgagtt cattaatgac acgttgtatc gttactgtct
     1021 ggatgagaag atcacgttta cccgttcacg cccttacaag aaaaacgatc aggcacatgt
     1081 cgagcaaaag aattggtccg ttgtcagaca cactgttggt tatgaccgtt gggaaacgga
     1141 actggaactt gccctcctgg aaagtattta tgacgaccta cggctctata tcaacttctt
     1201 tcagccatcc ttcaaactga tttccaaaga acgcattggc aatcggaccc tcaaacggta
     1261 tgatcgagcc aagacacctt accagcgtgt cctggaacgt aaggacgtat ctattgaatc
     1321 caaagcgcgg ctcatgaacc tgtatctcca gctcaacccc gccgaacttc gacgtcggat
     1381 tgaccagaaa actgccaaac tttggaaaat ttctcggtaa catttctgct tgatgcaacg
     1441 ttacctgcaa agtaacattt tatcttgatg cattacgcta agccctgaac cacgggtaca
     1501 ctaaaagtat gtagatgggt attcaaaagc gagcctctga gataggcttg ggtttggaca
     1561 acaacaaacc gaacccggag gctcaaaatg gagtgtagca caagacggat ggagcaggta
     1621 gcaaaattgt tggcagagga aattgcagag aaactggcag gcaagcagga gctcgatgaa
     1681 atggaaagca tgatgagaga gttggtcaga gaagcagcca atgtaggcct gcggcgggtc
     1741 atcgaacagg gtgaagaaac ttatggaagc agagaagtaa tctgtccctg tgggcagaaa
     1801 gcgcaatttg tcagtaaacg ctcagccgtg ttatggacgg tctttggaaa agtggactac
     1861 cagcgcagat attatctttg tcctacatgc catcaaggac aatgcccctt ggatctgaag
     1921 tatggcattg tgccaggaca gaccacacca accttggcga gtctgttagg catgttgggg
     1981 gtggaagtct cctttgcaga agcgagtcga ttggcagaac gctttctctt atttcgagtg
     2041 agtgataata ctgtgcgcaa gcaaaccgaa ggctacggaa acgcacaagc gcaggtagaa
     2101 caagaatgga tcgagaatgc ggaagatgaa aaagctttgc aagtccgcga gcgcagtttg
     2161 gaacctcagc cagggcggat ctatgcctcc atcgacggtg cccatgtgcc cctccagcaa
     2221 gaatggcggg aactcaagac cctgtgctgg tacaaagtgg agaaacttcg tccatctatt
     2281 ccgcgaaagc atcacgggca accaatggat ggacagagcg atttgcaggc caaggagatg
     2341 aagtatcact gcgacatcac cgaggcagag caattcggca ggctgctttg ggcaacgggt
     2401 ctgcaaaacc aggccgacac ttatgaggaa gtcgtttttg taaatgatgg tgcgctgtgg
     2461 atctggcgtt tagtggaaaa gtattttcca caagccgt
//
LOCUS       DBNHOH010000464         2378 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_475885, whole genome shotgun sequence.
ACCESSION   DBNHOH010000464 DBNHOH010000000
VERSION     DBNHOH010000464.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_475885"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..886)
                     /locus_tag="AC16V7_08110"
     CDS             complement(245..886)
                     /locus_tag="AC16V7_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257123.1"
                     /translation="MKQQFLFLFFAIALASCNSLAGNTQVIPPSVTTPEVAESSDQTP
                     SSGIESTVEISANETEFFSSDHLGLCFSYPKGYTQISFDDPILSVSIAAPDLPGTDTK
                     GHLSLEISDAYNRTAEEIADEDMTYAVDHAGVPPENLGRWTVTLGGEQAVVLDSMPGQ
                     DFRRQVYIVREQTLYMLGFSPVRSENKAANDQMEVLYEAVTRSWAWSPCSGVE"
     gene            complement(1046..1669)
                     /locus_tag="AC16V7_08115"
     CDS             complement(1046..1669)
                     /locus_tag="AC16V7_08115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6257124.1"
                     /translation="MMVNDFLHLDQTWECAEQSEITLVRSAQSGDVDSFNALVLKYQD
                     VMYRTGLRILGQEALAEDATQDALISAYHHIGQFRDGSLKAWFIRILVNKCYDDLRRM
                     QRTPAISLDESLTSDGENEGLYTVLWDQAPSVEEYVETSEKSEYIQKCLSKLPADYRV
                     VLVLVDIEEMSYVEASAILKIPLGTVKSRLARGRLYMRRELEAGGAF"
     gene            complement(1745..>2378)
                     /locus_tag="AC16V7_08120"
     CDS             complement(1745..>2378)
                     /locus_tag="AC16V7_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257125.1"
                     /translation="ISIMPLNVYALDSTGEKVWLIGNYMWQPFEHPVGSEFQFTTYTK
                     PAAGPVTIVVENAVAVYAPLYTDPPQAKPEEMTFSFDAGENPQYGQTWDVNNEFTIAG
                     YHIKVTSIRAASFKDIDALNFPFGSQGFEYGYDFMVEGDPSIKMQPLMKILSDSPVCY
                     LSNGDMTQPTSTPLHFIQLCRDTYPKGTVKVNIWQLSVLVENTWQVTWQP"
BASE COUNT          618 a          613 c          560 g          587 t
ORIGIN      
        1 aggtaggaaa gaaggagagg ttggtgttgt gacaagacca ggcgtgcagg cactcatcag
       61 ggtcactgcc agtaataaca agcaaagggt gtggataaac ttcattggta gtttcctcgg
      121 tctccaaaat gggatctttt ccctgagaat ctcgagtgac actttgcatg cggggcgaga
      181 gagatttccc agttgggatt catttctcac tccgatgcaa atagaacacg gacagcaatg
      241 gtgtctattc cacgcctgag cagggggacc atgcccatga tcttgtgacc gcttcgtaca
      301 ggacctccat ctggtcgttt gccgccttat tctctgagcg aactggcgag aacccgagca
      361 tgtagagagt ctgctcacgc acgatgtaca cctgacgccg aaaatcctga cctggcatac
      421 tatccaggac caccgcctgt tcgccaccga gcgttacggt ccaacgaccc aggttttcgg
      481 gggggacccc cgcatgatcg acagcgtatg tcatgtcctc atcagcaatt tcctcggcgg
      541 tgcgattata ggcatcgctt atctcaagcg agagatgccc tttcgtatca gtacccggaa
      601 gatcgggggc ggcgatgcta acagatagga ttgggtcgtc aaaggaaatt tgtgtgtatc
      661 ccttagggta ggaaaagcag agccccaaat gatcacttga gaaaaattcg gtttcattcg
      721 cggatatttc cacagtagat tcaatacctg atgaaggtgt ttgatccgaa ctctctgcca
      781 cctcaggggt tgtaaccgat gggggtatga cctgcgtatt ccctgccagc gaattgcaag
      841 atgctaatgc gattgcaaag aacaaaaaca gaaattgttg cttcataata ttgctccttg
      901 aagggtttgg gtaaacaaac tcttcgcatt ccataacgaa aaaattcctg aaaagttcca
      961 cacgatatct tcaagcggag gtcacgaggc agcaattgat aaatcaaaat gctggacatt
     1021 ttatcctgac cacattggtc tgcattcaaa aagctcctcc cgcttccaat tctcttcgca
     1081 tataaagccg tccccgcgcc aggcgactct tcactgtccc gagagggatt ttcaagatgg
     1141 cagatgcctc aacataagac atctcctcaa tatccacgag aacaaggaca acgcggtaat
     1201 cggccggcaa tttgctcaaa catttctgga tatactcgct cttttcggag gtttccacgt
     1261 actcctcaac cgatggcgcc tgatcccaaa gcaccgtata cagaccctcg ttctcgccat
     1321 cactcgtcaa actttcgtcc aaggatatgg caggagtacg ctgcatgcgc ctgaggtcgt
     1381 catagcactt gttgaccaaa atccgtataa accaggcttt gagcgaacca tcgcggaatt
     1441 gaccaatatg atgataggca gagattaatg catcctgcgt ggcatcctca gcgagagctt
     1501 cctgccccag gattcgcagc cctgtacggt acatcacatc ctggtatttc agcacaagcg
     1561 cgttgaaaga atccacgtcg cctgactgcg ccgagcgaac cagtgtaatt tcactttgtt
     1621 ccgcgcattc ccaagtttgg tccaagtgca gaaaatcatt aaccatcata gcctcaagaa
     1681 caaaacaatc tcttttcaag ctcgttccag cgaggggaaa ccctcgctgg aacacgtcat
     1741 ctatttacgg ctgccaggtg acctgccagg tgttttccac caaaacggag agctgccaaa
     1801 tatttacctt tacggttcct tttggataag tatcccggca aagttgaata aaatgtagtg
     1861 gcgtacttgt gggttgcgtc atatcaccgt tcgacaaata acataccgga gagtcagaca
     1921 ggatcttcat cagcggttgc attttgatcg agggatcgcc ttctaccatg aaatcatatc
     1981 catactcaaa gccttgagag ccaaacggga agttcaatgc atcaatatcc ttgaagctcg
     2041 ccgcgcgaat ggaagtcacc ttgatgtgat atccggcgat cgtgaactcg ttgttcacat
     2101 cccaggtctg accatattgc ggattttctc ccgcatcgaa tgagaatgtc atctcttccg
     2161 gttttgcttg tggcggatct gtatagagtg gcgcatacac agcaacggca ttctccacca
     2221 caatggtcac gggtcccgcc gcaggtttgg tgtacgtagt aaattggaac tcactaccca
     2281 ccggatgctc gaatggctgc cacatgtaat tgccgatcaa ccagaccttt tctccagttg
     2341 agtcgagtgc atatacgttc aacggcatga tcgaaatc
//
LOCUS       DBNHOH010000465         2542 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_925120, whole genome shotgun sequence.
ACCESSION   DBNHOH010000465 DBNHOH010000000
VERSION     DBNHOH010000465.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_925120"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2426)
                     /locus_tag="AC16V7_08125"
     CDS             complement(<1..2426)
                     /locus_tag="AC16V7_08125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR6257126.1"
                     /translation="MLVFIGLIVVVYMVQLQRQRPFVRVDRKLAMALITTGALTLGVI
                     AVQLIPLSEFWPRLSKSQDVVGSHSLRQIYLDYTSKDTFRPDAFSVLPARQDYYAYIG
                     IIPISALLLLPIALWKRERKPILVLSLMLLFVVLWVNLEHMPWYEPFLRIKLLMQFSN
                     LLRVLVFGSFAILLLGALGIDTLWKMLEAQISTNPTGGWEKGRALAANLGLVLLGGIM
                     LVSVGDLYQTHRKYIETQPFDQAVYTAARWLRQHDLSDYYVRFNPTNSAHEAVISADL
                     RFINAWYHFGDIRQFDNKLNTRPLQANATYVIQGSTDPPPASPTAKLVQQIEGFNIFR
                     LPDSLPYIFSVADTLLAPENNPAPLHSSEVTTQTSFSPGPNRVEVIASASENETLVVM
                     TTHYPGWKLEVDGHSHDLLNVGGYLGTKLLPGVHKYVFYFRPRTFTIGLIVSLLCASV
                     TLYLLIIDSLPTLRQGFSQLRSTPERLRNLGHRVRRKTYAGRMVEQAIYKKGKLELEK
                     PLELDENAPIRLELETLVPQSSPSKAAWRRWKWASADLVGTLLKAITLTGLLFAGSLM
                     VYAITRFYALDRFPIYFFGDEAVQVNYAENLIARGFHGPDGALMPLFVEVSGNRWAPL
                     ISVYFHALTLTLFGKSIMVTRATSAAISLLGAGAVGLILHKVFKARYAWAGVLLVAVT
                     PAWFLHSRTAFETVLTTSFYALFLLFYLLYYTESPRYLYAAVIFGAATFYSYSNAQLI
                     VLAAATLLFISDIRYHLRNRDIVLRGLLLAAVLAIPLIEFRLSHPQAIHEHLRVIGSY
                     WTQAIPLQNKL"
BASE COUNT          657 a          629 c          682 g          574 t
ORIGIN      
        1 agtttattct gcagtggtat ggcttgtgtc cagtaggagc caatcacgcg aagatgctcg
       61 tggatcgcct gcgggtggct caatcgaaat tcaatcaacg ggatcgccag cacagcagcc
      121 aggagcagcc ccctaagaac gatatctcga ttgcgcaggt ggtagcggat atcggagatg
      181 aatagtaacg ttgctgcggc caggacaatc agctgggcat tagaatacga gtagaacgtg
      241 gctgccccga agattacggc ggcatagaga tagcgcggcg actcggtgta atacagcaga
      301 taaaacaaca gaaaaagggc gtagaaggag gtggtcaata ctgtctcgaa ggctgtgcga
      361 gagtgtaaaa accaggctgg ggtcaccgcc accaacagca cgcccgccca ggcatagcgt
      421 gccttgaaga ccttgtgcaa tatcagccca actgccccgg cacccagtaa gctgatagcc
      481 gccgatgtcg cccgggtgac catgatagac ttcccgaaga gggttaaggt cagagcgtgg
      541 aaataaaccg agatcagcgg tgcccagcgg ttgcctgaga cttccacgaa tagtggcatc
      601 agggccccat ccgggccatg aaatcccctg gcaatcaggt tttcggcgta gttcacctgt
      661 actgcttcat cgccaaaaaa gtagattggg aagcggtcca gggcatagaa gcgtgtgata
      721 gcataaacca tcagggaacc agcaaacagc aaacctgtta atgtaatagc ttttagcagc
      781 gtgccgacca agtctgcgga ggcccatttc cagcgcctcc aagccgcttt gcttggtgag
      841 ctttgtggca cgagcgtttc gagttctaat ctaatggggg cattctcgtc gagttcgagc
      901 ggcttttcta gttctagctt gcctttctta tatattgcct gttccaccat acgtccggcg
      961 taagtttttc gtcgaacccg gtgacccaag ttgcgtaatc tctccggggt agatctgagt
     1021 tggctaaatc cttgtcgaag agttgggagc gaatcgatga ttaacaagta gagtgtgact
     1081 gatgcacaga gcaggctaac gatcagccca atagtgaatg tgcgcggcct gaaataaaag
     1141 acatatttat gtacaccggg cagcagcttg gttcccaggt atccacccac attgagcagg
     1201 tcgtgagagt gcccatctac ctccaatttc catcctggat agtgtgtggt catcacaacc
     1261 agggtctcgt tttctgaggc gctggcgata acctctaccc ggtttggtcc gggtgagaag
     1321 gatgtttgag tggtcacctc gctgctgtgt agcggagcgg ggttattttc cggcgccagc
     1381 agggtatccg ccacggagaa aatgtatggc agactatctg gcaagcgaaa gatattgaag
     1441 ccctcgattt gttgaactag ctttgctgtg ggtgatgcgg ggggcggatc ggtggatccc
     1501 tggataacat aggtcgcatt ggcttgcagg gggcgcgtgt tcagcttatt atcaaattgg
     1561 cggatatccc caaaatggta ccaagcgtta ataaaacgca gatctgctga tatcaccgcc
     1621 tcatgtgcgc tgttggtagg gttgaagcga acatagtaat ccgagaggtc atgctgcctc
     1681 agccagcgcg ccgcagtata taccgcctga tcaaatggtt gcgtctcgat atattttcta
     1741 tgtgtttggt aaagatcgcc cacgctcacc agcataatcc cacccaagag aaccaatccc
     1801 aggttcgcgg cgagcgcgcg ccccttctcc catcctccgg tgggatttgt gctgatttgg
     1861 gcttccagca ttttccaaag tgtatcaatc cctagcgcac ccagtagcag gatagcgaag
     1921 ctcccaaaaa ccagcacgcg cagcaggttg ctaaactgca ttaacaactt gatgcgcaga
     1981 aagggctcat accatggcat gtgctctaaa ttgacccaca aaactacaaa gagcagcatc
     2041 agtgaaagca ccaaaatggg ttttcgttct cttttccaca gtgcgatggg caatagcagc
     2101 agcgctgaga ttggaatgat gccgatatat gcatagtaat cttgtcgtgc tggtagcacg
     2161 gagaaagcat ctggtctgaa tgtgtctttc gatgtgtaat ctaaatagat ctgtcgcaga
     2221 gaatgcgagc caactacatc ttgggatttg gacaggcgcg gccagaactc gctcaacggt
     2281 attaactgga cggcgattac ccccagcgtt agcgcgccag tagtgattag tgccatggcg
     2341 agctttctat cgactcgcac gaagggtcgc tggcgctgga gttgcaccat atacaccact
     2401 acaatcaaac cgatgaatac caacatatag aacgcaaaat aaacattccc ggagaaaaat
     2461 aacaatgaca gggagataat cgtcccagcc caaaaaacac gccgcctagt cttaagtaag
     2521 cagaaaacgt tacctattgc cc
//
LOCUS       DBNHOH010000466         2196 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_668167, whole genome shotgun sequence.
ACCESSION   DBNHOH010000466 DBNHOH010000000
VERSION     DBNHOH010000466.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_668167"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="AC16V7_08130"
     CDS             complement(<1..1007)
                     /locus_tag="AC16V7_08130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6257127.1"
                     /translation="MMSVDVVWLLVFCALVVIAGVTSLNALTFPRLRSSALQAYPLVS
                     VLVPARDEAQVIAETVRRILAQDYPSFELILLDDHSSDGTADLAKAAAGDDTRMLILA
                     GQALPEGWTGKNWACWQLAGKAQGEILVFSDADVRWESGALTAIVAGLQATKADVFSA
                     WPTQRSGSWGERLVVPLMMFAVLGYLPEVAVRFVPLSALAAANGQCLAFRKEAYERVR
                     GHFSVRNRVVEDVEMARLSKASGLRLVMALGSEMVWCRMYHCWREVRDGFAKNILAGH
                     GHPLYLVASTLFHWLLFIVPWFWLLVGGWIDLGTGWPFAPLLLIGLGLGSRLLSAKVA
                     G"
     gene            complement(1004..1609)
                     /locus_tag="AC16V7_08135"
     CDS             complement(1004..1609)
                     /locus_tag="AC16V7_08135"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR6257128.1"
                     /translation="MDTIFWSLAGFLSGSIPFSLIIGRLGRDVDIRQYGDHNPGATNV
                     LRAAGWRWGIPAMLLDYFKGVVPVGLAWFFGDQVGWRIVPIALAPVLGHAFSPWLGLR
                     GGKAVAATFGVWTGLTLGAGPIMFGILLGLMYAVMVVSGWAVILAYLCFAIFLATVYR
                     GNPEFLVIWLGIFLVLCWKHRHDLRQRPAIRPGILRLVRLK"
     gene            complement(1573..>2196)
                     /locus_tag="AC16V7_08140"
     CDS             complement(1573..>2196)
                     /locus_tag="AC16V7_08140"
                     /inference="COORDINATES: protein motif:HMM:NF016155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HIR6257129.1"
                     /translation="ALIVLVLAWMIEWLGGTTGFPFGDYHYTEILRPKLGNVPWLIPI
                     AWLMMLVPSWAVGQAALGGIRERLKGLYWVTLAVISGAAFTSWDLYLDPQMTERGLWS
                     WEHPGGYFGIPWVNFGGWWLSAALITLILRPANLPQGPLIVIYTLTWAFQAVGLGIFW
                     GQPGPALAGFLVMGVFVLLAVIRVAPEGNHLRGWRGWIRYFGLWPDF"
BASE COUNT          589 a          713 c          524 g          370 t
ORIGIN      
        1 tgcccggcta cctttgcgct aaggagtcga gatcccaagc ccaagccgat caataataat
       61 ggggcaaatg gccagccagt ccccagatcg atccatccac cgaccagcag ccaaaaccag
      121 gggacaataa aaagcaacca atgaaacagg gtcgaggcga ccaggtaaag cgggtgcccg
      181 tgcccagcta ggatattttt ggcaaatcca tctcttacct cacgccagca atgatacatt
      241 cggcaccaaa ccatttcgct ccccaaggcc atcaccaggc gcaacccaga tgctttgctc
      301 agcctcgcca tctccacatc ttcaactacc cggttgcgga cagaaaaatg ccctctcaca
      361 cgctcatagg cttccttgcg gaaggccagg cactgcccgt tggctgctgc gagggctgac
      421 aggggtacga aacgtaccgc gacctcaggc aggtatccca gcacggcgaa catcatcagc
      481 ggcactacca gtctctctcc ccaggacccg ctgcgctggg tgggccaggc actaaataca
      541 tcagccttgg ttgcttgcaa acctgccaca atggcggtca gcgcacccga ttcccagcgc
      601 acatccgcat cggaaaagac caggatctca ccctgtgcct tgccagcaag ctgccagcat
      661 gcccaattct tcccagtcca gccctcgggt aaagcctgcc cggctaagat gagcatacgg
      721 gtgtcatcgc ccgctgccgc ttttgccaga tcggcagtgc cgtcgctgga gtgatcatcc
      781 agtaatatga gctcaaacga agggtagtcc tgcgccagta tcctgcgaac tgtttcggcg
      841 atcacctgcg cttcatcccg agcaggtacg agcaccgaca ccaagggata cgcttgcaaa
      901 gctgagctac gcaagcgcgg gaaggtcagc gcgttgaggg aagtcactcc cgcgatgacg
      961 accaacgcac aaaaaacgag cagccacaca acatccacgc tcatcatttc aggcgcacta
     1021 atctcaggat acccggacgg atagccgggc gctggcgtaa atcatgccgg tgtttccagc
     1081 aaagcacaag aaatatcccc agccagatca ccaaaaactc tggattgcct ctatagaccg
     1141 tcgccaggaa gatcgcaaag caaaggtaag ccaggatcac cgcccaccca ctgaccacca
     1201 tcaccgcata catcagccca agaagaatcc cgaacattat agggccggca cccaaagtca
     1261 agccagtcca aaccccgaag gtggctgcaa ccgccttgcc gccgcgtagc cccaaccagg
     1321 gtgaaaaggc atgccccagg acaggtgcca gggcaattgg cacaatcctc caaccgacct
     1381 gatcaccgaa gaaccatgcc agtcccaccg gtacaacacc cttgaaataa tctaataaca
     1441 tcgccgggat accccagcgc cacccggctg cgcgcagcac attcgtggcg cccggattgt
     1501 ggtcgccata ttggcggatg tccacatctc gccctagacg cccgatgatc aatgaaaagg
     1561 ggatcgaccc acttaaaaat ccggccagag accaaaatat cgtatccatc ccctccatcc
     1621 tcgcaagtga ttgccttcag gtgctaccct gattacagcc agcaggacaa atactcccat
     1681 caccaaaaaa cctgccagag ctggccccgg ctgtccccaa aagattccta accctactgc
     1741 ctggaatgcc caggtaagcg tgtaaataac gatcaaagga ccctggggga ggttagccgg
     1801 acgtagaatc agggtgatca aggctgcgct caaccaccat ccaccaaaat ttacccaggg
     1861 gatgccgaag tatccaccgg ggtgctccca gctccataga ccccgttcgg tcatttgcgg
     1921 gtcgaggtac aggtcccatg aagtgaaagc tgccccagaa atcacagcca atgtaaccca
     1981 gtataaaccc tttaagcgtt cacgaatccc acccagagca gcctgcccca cagcccagga
     2041 tggcaccagc atcatcaacc aggcaatggg gatcagccaa gggacattac cgagtttggg
     2101 gcgaaggatt tctgtgtaat gataatcacc aaatggaaac cctgtcgtcc cacccagcca
     2161 ctcaatcatc caggccagga cgagtacaat caaggc
//
LOCUS       DBNHOH010000467         1599 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1757842, whole genome shotgun sequence.
ACCESSION   DBNHOH010000467 DBNHOH010000000
VERSION     DBNHOH010000467.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1757842"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..1554
                     /locus_tag="AC16V7_08145"
     CDS             274..1554
                     /locus_tag="AC16V7_08145"
                     /inference="COORDINATES: protein motif:HMM:NF020130.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HIR6257130.1"
                     /translation="MNDQLDKPEDSQETISFFDRPGVQVQILPIGPGIPLGIEQVSSP
                     SLGIGGAYGTWGESYDNASLPSLVEHRLGAPLDESDRLDLSPLGFDRRHHITDLTDEE
                     HYQVEIQVGAQLLRAAAQACGWEPSEVDAVMIGLSGPIAEDYTEQIARTAGVPESALK
                     VSIHKACDGSVGALHLALNPDLPTHRLKGRNIAEELYGKKVLVGGIEGLSRFIRKTRD
                     KNAMQLFGNGAGVIGLIPGKTMKFLVGKTHEVFDDEGVLAVRMYYPHSRHTEEGGSMV
                     EVTQTGEHHIRIAGLMHEPEDASPVVMAGPMGMVKLFVRSGVDVVRDVYAAYQARLNE
                     LGMAGKQVATTVVHHANLKINRLKEKHLQKEGINLPMPWVIKEFGNVSAASNMIAFLR
                     QLPGLRPGDHVLFDGFGAGTYYDVLAVVLSETAA"
BASE COUNT          396 a          425 c          431 g          347 t
ORIGIN      
        1 atcttctcgc ctttcctttg ggatggttca gattatactt acacacaacc attggtcaag
       61 gtgacatttt tgctgaacag ttagggtgac attttccctg aacaatgaca ggagtaaatt
      121 gtcaatagtt gaaaattgct gtcttgacaa aacgaccagg tttaactaat atagccaaga
      181 cttaacgaag taacttatcc gcagtacgcg tccacccgcc agcttcttgt cggatggtgc
      241 tatttattaa tttggaaaga acaggtacaa accatgaatg accaattaga caagccagag
      301 gattcacagg agactatttc cttttttgat cgccctggag tacaagtgca aatcctgccg
      361 atagggcctg gcataccgct gggcatcgag caggtctctt ccccgtcact gggtattggc
      421 ggcgcatacg gaacctgggg agagagctat gacaacgcca gcttgccaag cctagtggaa
      481 caccgcctag gagcacccct cgacgaatcc gaccgcctgg atctatcgcc gttgggattt
      541 gaccgccggc accacatcac agatctaacc gacgaagaac attaccaggt tgaaatccag
      601 gtgggtgccc aactgcttcg tgccgccgcc caagcttgtg gctgggagcc aagcgaagta
      661 gatgccgtga tgattgggct ttccggaccg atagcagagg attacaccga acaaattgcc
      721 cgcacggccg gcgtgcccga gagcgcatta aaggtgagca ttcacaaagc ctgcgacggg
      781 tctgtgggcg ccctgcatct ggcgcttaat ccagacctgc caacccaccg gcttaagggg
      841 cgcaatatcg ctgaagaact ctacggcaag aaagtgctgg taggtggtat tgagggactg
      901 agccgcttca tacgcaagac gcgggataaa aatgccatgc agttattcgg caacggcgcc
      961 ggggtgattg gtttaattcc gggaaagacg atgaaattcc tggtgggcaa gacccacgaa
     1021 gtattcgacg acgagggcgt gctggctgtg cgcatgtact accctcattc tcgccacaca
     1081 gaagagggcg gatcgatggt cgaagtcacc cagacaggcg agcaccacat ccgtattgcc
     1141 ggtctgatgc acgagcccga ggatgcctca ccagtagtca tggcgggccc gatgggcatg
     1201 gtcaagctat tcgtgcgttc cggcgttgac gtggtgcgag acgtctatgc cgcttaccag
     1261 gcgcgcttga acgagcttgg catggctggc aagcaggtcg ccaccacagt tgtgcaccac
     1321 gccaacctga agatcaaccg tctgaaggaa aaacatcttc aaaaagaggg cattaacctg
     1381 cctatgccct gggtcatcaa agaattcggc aatgttagtg ccgcatccaa catgatcgcc
     1441 ttcctgcgcc agctcccagg gttgcgtcca ggcgaccacg tgctattcga tggttttgga
     1501 gcaggcacat actacgatgt gttagcggtc gttctaagtg agactgcagc ataaacatta
     1561 cctggcaacc gctttcttgc ctatcgcgag accccctat
//
LOCUS       DBNHOH010000468         1802 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1629192, whole genome shotgun sequence.
ACCESSION   DBNHOH010000468 DBNHOH010000000
VERSION     DBNHOH010000468.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1629192"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="AC16V7_08150"
     CDS             <1..250
                     /locus_tag="AC16V7_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257131.1"
                     /translation="VQPTSTPPVTSVGVSNLQDPRILWPMVLATLAPVLGGLVVLGLI
                     AIKPEILEQEERSRVIRVGFVVTLTVGAVLVMGVFGGI"
     gene            complement(608..775)
                     /locus_tag="AC16V7_08155"
     CDS             complement(608..775)
                     /locus_tag="AC16V7_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257132.1"
                     /translation="MVVGSAIAGLRLIPPKRASIFPRVRFLCRAPPFQRAGALAMKEL
                     AHNQATAKTLN"
     gene            780..1697
                     /locus_tag="AC16V7_08160"
     CDS             780..1697
                     /locus_tag="AC16V7_08160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR6257133.1"
                     /translation="MDFLHHRIVVVVPAYNEERFIGSVVIKILKYPVTVIVVDDGSTD
                     DTAYVAQAAGAVVVCHEKNQGKGQALNTGFCQARQLGPEAIVIIDGDGQHLPEQLPHL
                     VQPVLAGQADLVVGSRYLRKDCRVPRTRLIGHWFFNQLARHASGVGVSDSQSGFRAFS
                     PRAYNADLFHCSDFSVESEMQFLAREYGLRVAEVPITIRYEGPPKRPALQQGLVVLNG
                     ILHMVGQYRPLFFFGVPGAAFLLTGLGWGLWVVELFMQTKQLAIGHAFIFVLLFLTGQ
                     AMLSTGFILHSVRGLLHDMLRGRVKPNGG"
BASE COUNT          341 a          543 c          519 g          399 t
ORIGIN      
        1 ggtacaacca acatcaactc cccctgtgac cagcgtaggg gtatcgaacc ttcaagatcc
       61 gcgcattctt tggccgatgg tcttggcgac actggcccct gtgctgggag ggctcgtcgt
      121 cttgggcctc attgccatta aacctgaaat cctcgaacaa gaggagcgat ccagggtcat
      181 ccgcgtgggc tttgtcgtca ccttgacggt gggggccgtc ctggtgatgg gcgtcttcgg
      241 cggcatctag cgcggcgccg cggccggcat cctgggcacc gtcgctggct acctgctccg
      301 gggcgtcaaa gagggctgag ctcttcgtcc gcgccttccc gatcccccgc cggatggggc
      361 gtatccgcct cgtccaccct ttcttggctg acaagcttgc ttacaatttt tttataaaac
      421 aacatttttg gaaaattttc tgtatactct cattacacat tattgatata actggaatgt
      481 cacccattta acacggcgcg gtatggtcca tgagcttggc acaatgctct tctaggataa
      541 gctccggtag gtgggcctga acggggggca caatcagccg tagccaatcc ctcatatcaa
      601 agttacttta gttcagcgtc ttagcggttg cttggttgtg ggcaagttct ttcatcgcca
      661 gcgcgcctgc gcgctggaag ggcggagcac ggcaaaggaa acgcacacgg gggaatatgg
      721 aagcccgttt gggagggatc agccgcaagc cagcaatcgc ggagcccaca accatcccgg
      781 tggattttct ccaccaccgc attgtggtag tggtcccggc ttacaacgaa gagcgcttca
      841 tcggcagcgt ggtgatcaaa atcctcaaat atccggtcac cgtgatcgtg gtcgacgacg
      901 gatcgaccga cgacaccgcc tacgtggccc aggcagccgg tgcggtagtg gtgtgccatg
      961 agaaaaatca aggcaagggc caggctttga acacgggttt ctgccaggcg cgccagctgg
     1021 ggccagaggc catcgtcatc atcgacggcg acggacagca cctgcccgag cagctgcctc
     1081 acctggtgca acccgtactg gcaggtcagg ccgatcttgt ggtaggctca cgctacctga
     1141 ggaaagattg ccgcgtgccg cgcacccgcc ttatcggtca ctggttcttt aaccagctcg
     1201 cccgccacgc atcgggagtt ggggtcagcg attctcagtc cggcttccgc gccttctctc
     1261 cccgcgctta caacgccgac ctgttccatt gctccgactt ctccgtcgaa tcggagatgc
     1321 agttcctggc ccgcgagtac ggcctgcgcg tggcagaggt gcccatcacc atccgctatg
     1381 aaggcccgcc caagcgccct gccctgcagc aggggctggt ggtgctcaac ggcatcctgc
     1441 atatggtggg acagtaccgc ccgctgtttt ttttcggcgt gccgggggct gcgtttctat
     1501 tgaccgggct gggctggggc ctgtgggtgg ttgagctctt tatgcagacg aaacagctcg
     1561 ccatcggcca cgccttcatc tttgtcctcc tcttcctcac cggccaggcc atgctctcca
     1621 ccggcttcat tctgcactcg gtgcgcggcc tgctgcacga catgctgcgc gggcgcgtga
     1681 aacccaacgg tgggtagagg aattcgggct tgatcagcag cagagtatta tccgcaccct
     1741 tgagatgata tatctcaaaa tcgcaaccta taaaaatatt cggtcaatgc caacgtagtg
     1801 tg
//
LOCUS       DBNHOH010000469         1871 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1757888, whole genome shotgun sequence.
ACCESSION   DBNHOH010000469 DBNHOH010000000
VERSION     DBNHOH010000469.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1757888"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..732
                     /locus_tag="AC16V7_08165"
     CDS             169..732
                     /locus_tag="AC16V7_08165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010650240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HIR6257134.1"
                     /translation="MPVRTEKEKMLAGESYNCLDPDLDAERQKTKELFRLYNLTEDLA
                     ERQTILQQMLGHIGQNSIIEPPFYCSYGHNTHIGDHTYLNFMCTILDNNEVRIGDYVM
                     IGPVVQIYTAAHHLQAEPRNQGLEVAKPIVIEDNVWIGGGAIILPGVSIGRNAVVGAG
                     AVVTRSVPANTVVAGNPARVIREIEQL"
     gene            1364..>1871
                     /locus_tag="AC16V7_08170"
     CDS             1364..>1871
                     /locus_tag="AC16V7_08170"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6257135.1"
                     /translation="MSNFMKRICSKVWENGLTRTFQQFISVMEDYYFDLKHGIDTSEI
                     VQVEDLDITREDKKHSRRYQPTRIRYIKKLIRELKLPEGSVFVDFGSGKGRVLFVASN
                     YNFKRVVGVEISSKLCEIARNNLVVYERKLKRPLQVEIVESNVLRYDIRDDENIFYFF
                     RPFDGLIME"
BASE COUNT          531 a          362 c          459 g          519 t
ORIGIN      
        1 tggtcttcag gtttcaatac cgcccaacac cggctccaca tgaccgggta tgcgtcggtc
       61 attttaaatc aaagccgcca ggaggttgat taagaagttt tcccactctg atggcgaggg
      121 tgagcaatct gagaaatgcg cacaagatag tcaagcagga gtcataagat gcccgtaaga
      181 accgaaaaag agaaaatgct agctggagaa agctacaact gtcttgaccc tgatctggat
      241 gctgaacgcc aaaaaacgaa ggagttgttc cggctctata atctgaccga agatctcgct
      301 gaacgacaaa ccatactcca gcaaatgtta ggacacatcg gtcagaattc gatcattgag
      361 ccacccttct actgtagcta tggccataac actcatattg gggatcatac ctatctgaat
      421 ttcatgtgta ccattctaga taataatgag gtgcgtattg gtgactatgt catgattgga
      481 cctgtcgtcc agatctatac cgccgcccat caccttcagg cggaaccccg taatcaaggc
      541 ttggaagtag ccaaaccgat cgtgatcgag gacaatgtgt ggatcggggg tggtgccatc
      601 atcttgccag gggtgagtat aggccggaat gctgtggtgg gcgcaggggc agtcgttaca
      661 cggagtgttc cagcgaatac ggtcgtggcg gggaatccag ccagggtgat cagagagatc
      721 gagcagttat aactggctag agggctatta gggtacgctc catgggcgca ctctgctacc
      781 atggggttag tttaaccact tctcctttga aaacaagggg ggcataattt cggtctactc
      841 agctttcgct cctgacctcg cgcccttggt atccaagtcg gctggctctg tgccatttgc
      901 agatctcgtc aagaccctca aacagtatct gcccgattgc tctccgcttg accattgtga
      961 atggtacaga acgagatacc ttatgatgcc tgtcgcttat tattaagggg tggattgcct
     1021 cgggagttgg cgctgttcct tatagcagtg ataccgctat cctctcccat atcgggggtc
     1081 ggaccattac attgttaggc ttgaattcaa tctaccagta attttttgat ctcaacttcg
     1141 tttattaaaa agacaatttt gagtaaaata gggtagaaaa ttccaaagta aagaatttga
     1201 gccattggga tcttctaaat tcccaagact gcgctttgtg cgtaggcaat aacattttca
     1261 aagatcgaga agggttccca tatcgatcta ccaaaaccag aggtgatggt gatggtaagt
     1321 agtattgatt tcgcgagatg aacgtgcttt tgaggtatta caaatgtcga attttatgaa
     1381 gcgtatctgt agcaaggttt gggaaaatgg gttaacaaga acttttcagc aatttattag
     1441 cgttatggaa gattattact tcgatttgaa gcatggaatt gatacatcag agatcgttca
     1501 ggtggaagat ttagatataa cacgtgaaga taaaaaacat agtcgacgat atcaacctac
     1561 tcgcatcaga tatattaaaa aattgatcag agaattaaaa ttgccggagg gtagtgtatt
     1621 tgttgatttt ggatctggta aggggagggt tttatttgtt gcatcaaatt acaattttaa
     1681 gcgtgttgtg ggtgtcgaga tatccagtaa attatgtgaa atagccagaa ataatcttgt
     1741 ggtctacgag aggaaattga agagaccttt acaggtggaa atcgtagaat caaatgtttt
     1801 acgatatgac attcgtgatg atgaaaacat tttttatttc tttaggccat tcgatggact
     1861 aattatggaa a
//
LOCUS       DBNHOH010000470         6202 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_219187, whole genome shotgun sequence.
ACCESSION   DBNHOH010000470 DBNHOH010000000
VERSION     DBNHOH010000470.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6202)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6202)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6202
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_219187"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="AC16V7_08175"
     CDS             complement(<1..796)
                     /locus_tag="AC16V7_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012595446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR6257136.1"
                     /translation="MRVKLILPALTEAKSPFWRPIKYSLFPPLGLASLAGYLGPDDDV
                     TIQDEHVETLNLDDRPDLVVIQVYITSAYRAYEIADHYRRKGVYVCLGGLHVTSLPEE
                     AAQHADSIFIGPGEDTWPVFLEDFRAWFPKKVYRSHSRTLIGVPRVRRDLIKRHLYLV
                     PNSLVVSRGCPHHCDFCYKDTFFQGGRSFYTQAVDHALAEIERLPGRHLYFLDDHLFG
                     DPRFAASLFDGMRGMGRLWQAAGTVQSVLRPGLLEKAVECGLRSLFV"
     gene            complement(804..1535)
                     /locus_tag="AC16V7_08180"
     CDS             complement(804..1535)
                     /locus_tag="AC16V7_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257137.1"
                     /translation="MKERTRWIFVVLFAAAMAWLEAAVVLYMRLLLDRVQPYQIDPLP
                     VSVGLGKAEVVREVSTLIMLWAVGYLAGAERRSRLGYAMLAFGAWDILYYLFLVPLSG
                     WPDSLLDWDILFLIPLPWWGPVLAPASIALLMVMTGTLITQFQPSGQWLWPRWPAWLA
                     SLCGAGMALYTFMTDALRALGEGPIAVRQVLPVTFNWPLFGLALLLLSMPIIDLVWQL
                     WLPLSLGRVRYMSGKVPGRQETLGR"
     gene            1663..3030
                     /locus_tag="AC16V7_08185"
     CDS             1663..3030
                     /locus_tag="AC16V7_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257138.1"
                     /translation="MLDVLVFFQYIQAWEWITMNLLSEIQSARDDPKEMESLYQTACK
                     ADKCEEFRLALEACYQESPEDILYAAWHYRLKASLEQDSSQAAGVNWKLAVPLGIATG
                     LIFWGLSDFNVHFLDHLPYLALLWAPIATLFALGFLAFTAQRHYRRSLLVGIAVCLAS
                     AYVLLIAPSQKSGYHRHYLDLMIIHLPLLSWISLGISVLERGTRPRNKFAFLMKSIEV
                     MLTAGVYLVAGAVFAAITNGMFQALSIELPDITIRLITFGGIGLIPVLALASSYNPRF
                     RPQEQDFTQGLSRFVATLMRLLLPLTLAVLVIYILFIPFNFMEPFKNRDVLIVYNLML
                     FAIMGLLIGATPVRTEDLSLQLQKGLRYGILVVAILAVLVSIYALSATVYRTIEGGIT
                     MNRLTIIGWNSINIAILIVLIYKQFRYRGKNWANHLKSVFSLGMTGYVVWVIFLIVAI
                     PLLFR"
     gene            3037..3786
                     /locus_tag="AC16V7_08190"
     CDS             3037..3786
                     /locus_tag="AC16V7_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6257139.1"
                     /translation="MLEIILVEDNQRLRKALKSGLEATSQARQVFDSDRGEAALEYCL
                     EAQPEPSKTVILMDVQLAGEMNGIQAAVAIRREFPRFPVVFYSIQDEDVYYRDFRRSG
                     ILSHYAYVRKSNYLLPEMILPLLQDAIAGRSFIDAEIEARVQEVRLKDENDPMALLEP
                     NEQVVARLLAEGLTNEQIAARLGFRDKRTISRTNGQIYVAWGLTNSTTDEKVARTRAV
                     LIVQSGQLLSWDEGGVAHVLNEQGDWIPWVQ"
     gene            3797..5917
                     /locus_tag="AC16V7_08195"
     CDS             3797..5917
                     /locus_tag="AC16V7_08195"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6257140.1"
                     /translation="MSGNFYLDWAIQAISLFNTILLLWLGLTVLLNAERRTPGLWIAG
                     AQLLMGAAFFLSHSIILGYGQFVFGPGVNFWWRVGWIPVIGVPYTWYGVMLWYAGYWS
                     EPVSELRRRHRYWFMITTGLVALVFGLLIFANPLPTFSQVAELKLNATPSIGGIPLLI
                     LIYPLYIATCISLSIDVLRRPAPSGRLMGDLARRRARPWLFAASFVQLGVSILVGLVM
                     LGVILSAGRRVYDHTMAVTVAWFDLVIAGLITSAIVLVGQAIVSYEVFTGKPLPRGGL
                     RRYWQRLIILAAGYAVAVSFSLTLPLKPIYSLMLSTALMIAFYALLTWRSYNERESYI
                     ERLRPFVASQRLYEQITASSLQSPPELDAYPPFQALCADLLGAQCAYLIPLGPLAPLA
                     GPPLVYPQAKDAPLPGLGEIIPQWTNPQDLCTPLDPGVFYGAAWGIPLWSERGLVGGL
                     LLGEKVDGSLYTQEDFEIARAVCERLVDTQASAEMARRLMALQRQRLAESQVLDRQTR
                     RALHDDVLPRLHAMMLDLSGGKLPPHTTDEMMKSLGELHHQISDLLRELPPATAPQIT
                     RLGLIEALRQVVDGELKGAYDQVEWHISSDADARARDLPPYKAEVLFYAAREALRNAA
                     KHAGWQQAAAPLYLRIRARWHDGFELSIEDNGRGVVGSGDDQRGSGQGLTLHSTLMAV
                     IGGSLEMESTPGVSTCVILRIPDV"
     gene            complement(5945..>6202)
                     /locus_tag="AC16V7_08200"
     CDS             complement(5945..>6202)
                     /locus_tag="AC16V7_08200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6257141.1"
                     /translation="GLPVIVAEGDGTQDDLVRPENGWRVPPGDLGALTRTLQDALTDA
                     SRLRHMGRQSYRIVAEEANLERMVEVFVNALSSMKIGYRGD"
BASE COUNT         1304 a         1764 c         1740 g         1394 t
ORIGIN      
        1 ccacgaacag gctgcgtaag ccgcattcga cggctttttc taacaagcca ggtcgcaaca
       61 ccgactgcac cgttccagca gcctgccaca gccgccccat gccgcgcatc ccgtcgaaaa
      121 gtgaggcggc gaagcgcgga tcgccaaaca ggtggtcgtc gaggaaatac aggtggcgtc
      181 ctggtagtcg ctcgatctca gccagagcat gatccactgc ctgggtatag aacgacctgc
      241 cgccctgaaa aaaggtatct ttatagcaaa agtcgcagtg atgtgggcag ccgcgcgaga
      301 ccaccaaaga attcggcacc agatacaggt ggcgcttgat cagatctcgg cgcacccttg
      361 gcacaccgat cagggtgcgt gagtgcgagc gataaacctt tttggggaac caggcacgaa
      421 aatcctccag gaaaaccggc caggtatcct ccccggggcc gatgaagatg ctatccgcgt
      481 gctgggcagc ttcttcgggg agggaggtga catgcagccc gcccaggcat acataaaccc
      541 ctttccgtcg atagtggtcg gcgatctcat aggcacgata ggccgatgta atatacacct
      601 ggatcacgac caggtcgggc cggtcatcaa ggttaagcgt ttctacgtgc tcatcctgga
      661 tggtgacatc gtcatcggga cccaagtagc cagccagaga tgccagcccc aatggcggga
      721 agagcgagta tttaatcggt cgccagaagg ggctcttggc ttcggtcagc gcgggcagga
      781 tcagttttac tctcatgatt gggctatctt ccaagcgttt cttggcgacc gggcactttt
      841 ccgctcatgt atctcaccct gcccaaagaa agcggaagcc atagctgcca gactagatct
      901 atgattggca tcgagagcag cagtaaggcg agcccaaaca acggccagtt aaatgtgacc
      961 ggcaacacct ggcggacagc tataggtccc tctcccaggg cgcgcaacgc atcggtcatg
     1021 aaggtgtaga gcgccatgcc tgccccacag agacttgcca gccaggcagg ccagcgaggc
     1081 cacagccact gaccggatgg ttggaattgt gtgatgaggg taccggtcat gaccatcaat
     1141 aaagcaatgc ttgcaggggc aagcaccggc ccccaccagg gtagggggat caagaagagg
     1201 atgtcccagt ccaggagcga gtcaggccat ccgctcaacg gcaccaggaa aaggtaatac
     1261 aggatatccc aggcgccaaa agccagcatg gcatagccta aacgcgagcg gcgctctgct
     1321 cctgccaggt agccgacagc ccataacatg attaatgtgg atacctcgcg aaccacctcg
     1381 gctttcccca atcccaccga gactggcaat ggatcgatct ggtagggctg cacccggtcg
     1441 agcaataatc tcatgtagag cacaacagcc gcctctaacc acgccatcgc agcggcaaac
     1501 aggacaacaa agatccagcg agttctttcc ttcattacta aacctcctag cttcatcagc
     1561 tcttcagcaa agtccgctca tgctcgaatg agcatccaga tccatcttag gtgcctcacc
     1621 agataaactc cacctttcaa aaccgcagtc tggtgcactc aggtgcttga cgtgcttgtg
     1681 tttttccagt acattcaagc ctgggagtgg attaccatga atttattgag cgagattcaa
     1741 tccgcgcggg acgatcctaa ggaaatggaa tctctgtatc agaccgcttg caaggctgat
     1801 aaatgcgaag aattccgcct cgcgctggag gcatgctacc aggaatcccc cgaggatatt
     1861 ctgtatgccg cctggcatta ccgcttaaaa gcttcccttg agcaggactc atcccaggct
     1921 gccggggtca attggaagtt agctgtccct ttgggaattg ctacaggctt aatattctgg
     1981 gggctctccg acttcaatgt gcattttctg gatcacctgc cctacttggc gttgctttgg
     2041 gcgcccatag ctaccctgtt cgccttaggt tttctggcat ttacggcgca gcgccattac
     2101 cggcgtagtc tcctggttgg gatagccgtc tgtctggcaa gcgcatacgt gctgctcatt
     2161 gccccgtctc aaaaaagtgg ctaccaccgt cattatctgg atttgatgat cattcacctg
     2221 ccattgctga gctggatttc cttggggatc agtgttctgg aacgcggcac cagacctcgg
     2281 aataaattcg cgttcttgat gaaatccatt gaagtgatgc tcactgctgg tgtatatctg
     2341 gtcgccggcg cggtttttgc cgccatcacc aatggaatgt tccaggcgct cagtattgag
     2401 ctgccagaca ttaccatacg gttgattacc ttcggcggta taggcctgat tcctgtgctg
     2461 gcgctggcca gctcctataa ccccagattc agaccgcaag aacaggactt cacccaaggg
     2521 ttaagccgtt tcgtcgctac cttgatgcgc cttctgctgc cactcaccct ggcggtgttg
     2581 gtcatttaca tcctattcat accattcaac ttcatggagc ctttcaaaaa ccgtgatgtg
     2641 ctcatcgtgt ataacctgat gttgttcgcc atcatgggcc tactgatcgg tgctacacca
     2701 gtgagaacgg aagacctatc gctgcaactg caaaagggat tgcgctatgg cattctggtt
     2761 gtggcaatct tggctgtcct ggtcagtatt tatgcactct ctgccactgt ttaccgtacc
     2821 atagagggtg gtatcaccat gaaccgcctg accatcattg gctggaacag catcaacatc
     2881 gccatcttga tcgtgctcat ttataaacaa ttccgatatc gggggaaaaa ttgggccaac
     2941 cacctgaaat cagtatttag cttggggatg accggttacg tggtttgggt catatttctg
     3001 atagtcgcca tcccgttact gttcaggtaa gggcgtatgc ttgaaatcat ccttgtcgaa
     3061 gataatcaac gcctgcgcaa agccttaaaa tccgggctgg aggccaccag tcaggcgcgc
     3121 caggttttcg atagcgaccg cggcgaagct gccctggagt actgcctgga agcgcagcca
     3181 gagccatcta aaaccgtcat cttgatggat gtgcagttgg ctggggagat gaacggcatc
     3241 caggcagcag tcgccatccg gcgagagttt cctcgctttc cagtcgtttt ttattcgatt
     3301 caggacgagg atgtctacta ccgtgatttt cgccgctctg gtatcctcag ccactacgct
     3361 tatgtgcgca aatccaacta cctgctccct gagatgatcc ttccgctgct gcaagatgcc
     3421 atcgccgggc gcagctttat cgatgcggag atcgaggcgc gcgtccagga ggtgcgtttg
     3481 aaagacgaaa acgacccgat ggcgttgctg gagccaaacg agcaggtcgt cgctcgtctg
     3541 ctggcggagg ggttgaccaa cgaacaaatc gccgcccgtc tgggtttccg cgataagcgc
     3601 actatcagcc gcaccaacgg gcagatatat gtcgcctggg ggttgaccaa ttcgaccacc
     3661 gatgagaagg tggcgcgcac ccgggcagtg ctgatcgtcc aatcaggcca gctgttaagc
     3721 tgggacgaag gcggcgtcgc ccatgtgctg aacgagcagg gcgattggat tccttgggtg
     3781 cagtgatcgg cgaaagatga gcggtaactt ttatctcgac tgggcaatcc aggccatctc
     3841 actgtttaac accatcctgc tgctctggct gggtctcacg gtgctgctca atgccgagcg
     3901 acgcacgccg ggactgtgga ttgctggggc acagctgctc atgggtgctg ccttcttcct
     3961 gagccattct atcatcctgg gatatggaca atttgttttt ggtccaggcg tcaatttttg
     4021 gtggcgggtg ggttggatac cggtgattgg cgtgccatat acctggtacg gagtcatgtt
     4081 atggtacgct ggctactgga gcgaaccggt ctcggagttg cgccgccgcc atcgctactg
     4141 gtttatgatc accaccgggc tggtggctct ggtctttgga ttgctgatct ttgccaaccc
     4201 cctgcctact ttcagccagg tggcagagct gaaattaaat gccaccccct ctattggtgg
     4261 aatcccgtta ttaatcctga tctacccgtt gtatatcgcc acctgtatca gcctctcgat
     4321 tgatgtgctg cgccgcccgg ctccatccgg tcgtttgatg ggggatttgg cgcgccgccg
     4381 agcccgccca tggttgttcg cagcttcgtt tgtgcaactt ggggtcagca tcctggtagg
     4441 gctggtgatg ctgggggtta ttttgagcgc cggtcgacgg gtgtacgacc acactatggc
     4501 agtcactgtg gcctggttcg acctggtcat cgcaggcctg atcaccagcg ccatcgttct
     4561 cgtcggacag gcgattgtct cctacgaagt ttttaccggc aaacctctac cccgcggcgg
     4621 cttgcgacgc tactggcagc ggttgattat cctggcggcc gggtacgccg tggcggtcag
     4681 tttcagcctg accctacccc tgaaacccat ctacagcctg atgctgagca ctgctctaat
     4741 gatcgctttc tacgccttgc tcacctggcg atcttacaac gagcgagaga gttacatcga
     4801 gcgcctgcga ccctttgtcg ccagccaaag gctgtatgag caaataactg cttcctctct
     4861 ccagtcgccg ccagaattgg atgcttatcc cccgtttcag gcgctatgcg ccgatctatt
     4921 gggggcgcag tgcgcgtacc tcatccctct cggcccgcta gcaccgctgg caggcccacc
     4981 gttggtatac ccgcaagcaa aagatgcccc gctgcccggt ctgggtgaaa tcatccccca
     5041 atggaccaat ccgcaggacc tctgtacccc actcgatccg ggagtatttt acggggctgc
     5101 ctggggcatc ccactgtgga gcgagcgcgg cctggtaggc gggctgctgt tgggtgaaaa
     5161 agtcgacggg tcgctgtata cgcaggagga tttcgagatc gcccgcgccg tctgcgaacg
     5221 gctggtagat acccaggcca gcgcagagat ggcgcgccgc ttgatggcgc tgcaacgcca
     5281 gcgccttgcg gagagccagg tgctcgacag gcaaactcgc cgcgccttgc acgatgatgt
     5341 cttgccccgc ctgcacgcca tgatgctgga tttgagcggg gggaagctgc ctccacacac
     5401 caccgatgag atgatgaaat ccttggggga actccaccac cagatttctg acctgctgcg
     5461 cgagctgcct cctgccactg ccccgcaaat cacgcgtctg gggctgatcg aggcgctgcg
     5521 ccaggtggta gatggcgagt tgaaaggtgc ctatgaccag gtcgaatggc acatctcgtc
     5581 ggatgctgat gcgcgtgctc gtgatttacc cccatataaa gcagaagtgc tcttttacgc
     5641 ggcgcgggag gccttgcgta acgctgccaa acatgctgga tggcaacagg ccgctgcgcc
     5701 actatacctg cgcatccgtg caaggtggca cgatggtttt gagctgagta ttgaagacaa
     5761 tggacggggg gtggttgggt caggcgacga tcagcgcggc agcggtcaag gtctcaccct
     5821 gcatagcacg ctgatggctg tgatcggcgg gtcgttggaa atggagagca cgccgggcgt
     5881 atccacctgt gtgatcctga gaatccccga tgtttaaaca agatttatgg ggagtatcta
     5941 cgcactaatc cccacgataa ccaattttca tacttgatag cgcgttgaca aatacttcca
     6001 ccatgcgttc caggttggct tcttcagcca cgatgcggta agattggcga cccatgtggc
     6061 gcaggcggga ggcatcggtg agggcatctt gcagcgtgcg ggtcagcgcc cccaggtcgc
     6121 ccggcggcac tcgccagccg ttctcggggc gcaccaggtc atcctgcgta ccgtctccct
     6181 cggcgacgat caccggcagt cc
//
LOCUS       DBNHOH010000471         2099 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_27376, whole genome shotgun sequence.
ACCESSION   DBNHOH010000471 DBNHOH010000000
VERSION     DBNHOH010000471.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_27376"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="AC16V7_08205"
     CDS             complement(<1..473)
                     /locus_tag="AC16V7_08205"
                     /inference="COORDINATES: protein motif:HMM:NF012703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HIR6257142.1"
                     /translation="MSAKNPNPAFGAHLEPFHGITTFMRLPASRQLEGVDVAITGIPF
                     DSGTSYRSGTRFGPRKIRESSLLLWGFNTVLGVSPGETLKIVDYGDIEIIPVEISKTM
                     GNITQEISHLLQNGVTVIALGGDHSVSRPLLRAHAAQFGPLAVIHFDSHPDTWDQ"
     gene            complement(605..1003)
                     /locus_tag="AC16V7_08210"
     CDS             complement(605..1003)
                     /locus_tag="AC16V7_08210"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6257143.1"
                     /translation="MEILSASLEDLDQISELARIVWYASFIGMISEGQIEYMLSLKYT
                     PEKLRADILDSGAIYLKLVERDQLVGFASFRSSDELNLLLLDKLYVHPAQQRKGNGTR
                     LMSQAKKRRSKKDIRESFSPLSLRPTRDVT"
     gene            complement(1033..1416)
                     /locus_tag="AC16V7_08215"
     CDS             complement(1033..1416)
                     /locus_tag="AC16V7_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008191135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6257144.1"
                     /translation="MKTTTLPEHPDAKSPAGADIRFLMDGKTGNMIHSTVPPHQVNKA
                     TVHATVSEFWYVLEGHGEIWRDNGLESSITALVPGTSIDIPVGTAFQYRNVSDVEMKF
                     ICIAMPRWPGDSEATYVKGIWQPTV"
     gene            complement(1729..>2099)
                     /locus_tag="AC16V7_08220"
     CDS             complement(1729..>2099)
                     /locus_tag="AC16V7_08220"
                     /inference="COORDINATES: protein motif:HMM:NF013087.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HIR6257145.1"
                     /translation="KVAVGLGIANQVVLMPGDLWSVDLGSDAFDVAYLGNVTHFFSPE
                     ENTRLFRKVYSALAPGGCIVVNSMVRRESEWYSVALWLYAATATGGAYDFTEYRIMLE
                     NAGFANVRDFDQGPIKAVKP"
BASE COUNT          481 a          565 c          539 g          514 t
ORIGIN      
        1 tcctgatccc aggtatcggg gtgtgaatcg aagtggatga ctgccagcgg accaaattgg
       61 gcggcatggg cgcgcagtag cggtcggcta accgagtggt cgccccccaa ggcaatcact
      121 gtaacgccat tctgcaacag gtggctgatt tcctgggtga tattgcccat tgtttttgaa
      181 atctcaactg ggatgatctc gatatccccg taatcgacaa tcttgagcgt ttcccccggg
      241 gagacgccaa ggacggtgtt gaatccccac agcagcaagg aagattctcg tattttgcga
      301 ggcccaaagc gggtgccgct ccggtaactt gtgccactgt cgaaggggat gcctgtgatg
      361 gcgacatcca cgccctccag ctgacggctg gctggcaggc gcatgaaggt cgtgatccca
      421 tgaaatggct ccaggtgggc accaaaggct gggttgggat ttttcgcgct catactgttt
      481 tcctctacga tttgtatgcg agatacgggg ttacaatgcc gctgaatttc ccaggattgt
      541 aatttgggct gcaagcagcg tcaagcactc acggcgcgaa cctcgacttt gctatctctt
      601 gcccctaggt tacatctctg gtcggcctaa gcgagagagg cgagaaggat tccctgatat
      661 ccttcttact ccgccgcttc ttcgcttgag acatcagcct ggtgccgttg cctttccttt
      721 gttgtgcggg gtgaacataa agcttgtcaa gaagaaggag attcagctcg tctgaagatc
      781 tgaacgatgc aaagcctacc agctggtctc tttcaacaag cttcagataa atagcaccgc
      841 tatctagaat atctgctctc aacttctccg gggtatattt cagcgacagc atgtactcaa
      901 tttgaccctc ggagatcata ccaatgaagc ttgcgtacca tacaatccgg gctaactcgg
      961 agatctgatc gagatcttca agtgaagcac tcagtatttc catcgattac cggcaacgaa
     1021 tatcggacag cctcaaaccg ttggctgcca aatccccttg acataggtag cctctgagtc
     1081 tcccggccag cgcggcatgg cgatgcaaat gaacttcatt tctacgtcgg atacgtttcg
     1141 gtattgaaag gctgtgccta ccggtatatc gattgaggtg cctggaacga gagcagtgat
     1201 gctgctctct aagccattat ctctccagat ctcaccgtgc ccctccaaca cgtaccagaa
     1261 ttcgctgacc gttgcgtgaa cagtggcttt atttacttgg tgcggaggga ctgtactgtg
     1321 gatcatgttg cccgttttgc catccataag gaaacggatg tctgcaccgg ctggcgattt
     1381 cgcatcgggg tgctctggaa gggttgtagt cttcatattc tctcctcaaa tctgatctgc
     1441 ttagcgagta aattcagcct gctccataat ctgcgggtgt accacaccta gaaaatacat
     1501 cccggaatct tccaatccac agttccggca aacccgctgc gatggaatat tatctgcgcg
     1561 aacgccagta tgcactaact ctgctgaata ttcttcataa caggccagta tgatccgggc
     1621 aagtaacaag cgggcgtaca gcaagacgtg cgtctgtaaa ttgttttcca gcgcctgggc
     1681 gatatctcag gcggaggatt caagcagggt atcgcccttc ccaaactttc agggttttac
     1741 cgccttgatc ggtccctggt cgaaatctcg gacgtttgcg aagccggcgt tttcgagcat
     1801 gattctgtat tcggtgaagt catatgcccc gcccgtagcg gtagccgcgt agagccacag
     1861 agcaactgag taccactcgc tttcccgccg aaccatagaa ttgaccacaa tgcagccgcc
     1921 aggcgccagg gctgaatata ccttgcggaa caggcgggtg ttttcctccg gactgaagaa
     1981 atgggtgaca ttgcccagat aggctacatc aaaagcgtca ctaccgagat ccactgacca
     2041 caggtcgcca ggcataagca ccacctggtt ggctatcccc aatcccactg cgactttga
//
LOCUS       DBNHOH010000472         2289 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_155224, whole genome shotgun sequence.
ACCESSION   DBNHOH010000472 DBNHOH010000000
VERSION     DBNHOH010000472.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_155224"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="AC16V7_08225"
     CDS             complement(<1..636)
                     /locus_tag="AC16V7_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257146.1"
                     /translation="MIIEFIGTPGAGKTTLLPVVMDYFDGLGFRAYTVVQAARPFAAR
                     TLLGRAISRLAPAGLRQQLLWQVFYHLSKLYRLRFMLKNRELIYSVQRFQRKRPITSQ
                     DREHVLYWFIHLIGYYEFLKSQLEPNEALIFDEGFIHRVVQLFASEVEEPDPDQITAY
                     INLIPKPDLVVFPYSPKEICEKRVYARGIWDRFRLKSREEISRYMANSHEIV"
     gene            complement(665..2053)
                     /locus_tag="AC16V7_08230"
     CDS             complement(665..2053)
                     /locus_tag="AC16V7_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HIR6257147.1"
                     /translation="MQGLTDLDLLVDPEDAQVFKQVLAEHDIKPFLAAPGKRYPALED
                     YMGFDPSSGKFFHLHVHYQLVLGEQFVKNYRLPLEDQFLDSIQYRSGVKIPAPELELI
                     VLSIRALLKYRDRDSIKDILSIRSPGIPAHIMGEINWLLNQTCLNKVSKTLDEVADAI
                     SPDLILDFLNTVMMTPRAGYRLFRLRQSVRRAISVYQRHNRVWASLTYFKEFWRRRKS
                     FLRFMPERKMTNPRGGLTLALVGVDGAGKTTISRELSAWLDWKLDIHTYYLGSKQPSW
                     MSDRSYLLFRIARRSHRSISKVLGEKNFISRLIEALRQALLYSHYLFTGYDRYRRYLA
                     GNRNAKNGSISLYDRYPLEAPLDGPQIHLASENDPWRIGDIFSKLERKLYQEIKMPDL
                     LFVLEVSPEVSLERKPDHDLAAIEAKNIALGKLATFIEASPKKVRVSYINASLPLEDV
                     LGKIKQEIWGAL"
BASE COUNT          596 a          526 c          547 g          620 t
ORIGIN      
        1 gacgatctcg tgtgaattgg ccatgtaacg ggaaatctct tcacgggatt tcagacgaaa
       61 tcgatcccaa atgcctcggg cgtaaacgcg tttttcacat atttctttag gggaatacgg
      121 aaagactacc aggtcgggct tggggataag gttgatatac gctgtaattt gatcggggtc
      181 aggttcttcg acctccgagg caaagagttg tactacccgg tgaataaacc cttcatcgaa
      241 aataagtgct tcgttcggtt ctaattgcga cttcaagaac tcgtaatacc ctatcaaatg
      301 gataaaccaa tacaggacgt gttctcgatc ttgactggta atcggtcgct ttctttggaa
      361 tctctgcacg ctgtagatta gttcccggtt tttgagcata aatctgagcc ggtatagctt
      421 gctgaggtgg tagaacactt gccacaataa ttgctgccgc aaacccgcag gagccagccg
      481 gctgatagcc ctgcctaaca atgtcctggc ggcgaaaggt cgggctgctt gaacaaccgt
      541 atatgcccta aagccaaggc catcgaaata atccatcacc acgggcagga gggttgtttt
      601 gcccgcccca ggcgtgccta taaattctat gatcatgaat ggtttgaagt gaaaaaaagg
      661 tgctttacaa tgccccccat atctcctgct ttattttccc caggacatcc tcgagcggaa
      721 ggctggcatt gatgtacgat acccgaactt ttttcggtga tgcttcaatg aaagtggcca
      781 gtttccctag tgcgatattt tttgcttcga ttgccgccaa gtcgtgatcg ggttttcgct
      841 ctaaggagac ttctgggctc acttcgagaa caaagagaag atcgggcatt ttaatttctt
      901 gataaagctt ccgctctaac ttggagaaaa tgtcgccaat ccgccaagga tcattctcgc
      961 tggcgaggtg gatttgcggt ccgtccaatg gcgcttccaa tggatagcga tcataaaggc
     1021 tgattgagcc attttttgca ttacgattgc cagccaggta tcgacgataa cgatcatagc
     1081 cggtgaaaag ataatgactg tatagcagag cctggcggag agcttcaatc agcctgctga
     1141 taaagttttt ttccccaagc actttgctga tacttcgatg gctcctgcga gctatcctga
     1201 atagcaggta agagcgatcg ctcatccaag aaggctgttt gcttccaagg taataggtgt
     1261 gaatatccag cttccaatcc aaccaagcag acaactcgcg gcttatagtt gtctttccag
     1321 cgccatccac accaaccaac gccagggtca atccacccct ggggttggtc attttccttt
     1381 cgggcataaa tcgcaggaac gattttcgtc gccgccagaa ttctttgaaa taggtcaatg
     1441 aggcccacac gcggttgtgc cgttgataaa cgctgatggc tctgcgcacg ctctggcgca
     1501 atcgaaacag tctataccct gccctgggag tcatcatcac ggtgtttaga aaatccagga
     1561 tcagatcagg ggaaatggca tcagctactt cgtctaatgt tttggagacc ttgttgaggc
     1621 aggtttgatt caatagccag ttgatttctc ccataatatg agcgggtatg ccgggtgatc
     1681 tgatagaaag aatgtcttta atggaatccc ggtctcggta cttaaggaga gcccgaatag
     1741 agaggacgat cagttctaac tcaggcgcag ggatttttac acccgatcta tattgaattg
     1801 agtctaggaa ttgatcttca aggggtaagc ggtaattttt gacaaattgt tcgcctaaga
     1861 caagctgata atgaacgtgt aaatgaaaga acttgccgct gcttggatca aatcccatat
     1921 aatcttcaag ggctgggtag cgtttaccag gggcggctaa aaacggctta atatcgtgtt
     1981 cggcgagcac ctgcttaaag acctgagcat cttcagggtc cacaagcaga tcaaggtctg
     2041 tcagaccttg catgccccat tccaatctca aattgctttt ccagtggcaa tacctgatac
     2101 cagaatggtt taatgcctgg aataaaccct caactgtttg aagaatattt gtcttgtctt
     2161 cgagaggtgt ctgaattcta acagtggtat cctgaatcat agtcctcgaa ctgagccgtg
     2221 aatcaataat cgcaagtaat cttactcgtt gaaagaatat ccttgtaaga agtcgtactg
     2281 ccctatttt
//
LOCUS       DBNHOH010000473         4466 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1694496, whole genome shotgun sequence.
ACCESSION   DBNHOH010000473 DBNHOH010000000
VERSION     DBNHOH010000473.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1694496"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1334
                     /locus_tag="AC16V7_08235"
     CDS             <1..1334
                     /locus_tag="AC16V7_08235"
                     /inference="COORDINATES: protein motif:HMM:NF014740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HIR6257148.1"
                     /translation="GKQKQQIHGVSVVGRLDELARVISVRHVDEVVIAIPSAPGSVIR
                     QVADVCRLKSLPFRTMPGIFELIGGTVSVSRLREVEITDLLRREPTKIDEKIIGSSLG
                     GRRVMVTGAGGSIGRELCIQTARWGPSELILLGHGENSIFETLIEIEENFPSIPIHPV
                     IADIRDLDRLSVVFDDLRPQVIFHAAAHKHVPLMEANVEEAVSNNVLGTRNIVEVSQN
                     FGIERLVMISTDKAIRPTSVMGATKRLAELIVLDAAIRTHQAFSVVRFGNVLGSRGSV
                     IPKFKRQIAKGGPVTITHPDMERYFMTIPEAVHLVLQASSMGEGGEVFVLNMGQQVRI
                     LDLAEDLVRLSGLEPYKDIDIIFTGIRPGEKLSEDLWDQWVQFEPTSHPDILLLNDED
                     IISGQELRLKVNDLLHLAREGDSTAIISILDEFIPGAAVRSTPPPDMTSVV"
     gene            1436..2443
                     /locus_tag="AC16V7_08240"
     CDS             1436..2443
                     /locus_tag="AC16V7_08240"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase FemX"
                     /protein_id="HIR6257149.1"
                     /translation="MPEVSASEWDKFLSSYKDVHILQTSNWGELKKAFGWQVVRFVCT
                     APSQCGAQILFRRLPFGISLAYIPKGPVGKEWSALWSEIDSVCLQRKAIFLKVEPDLW
                     DGQALDNVQGDAPAGFQVGVQTIQPPRTLVVNIDGDEAQVMARMKQKTRYNIRLSHKK
                     GVVVRPSADLQLFSTLMRITAERDRFGAHSQAYYEKVYDLFHPRGECELLLAEYEGEP
                     LGALMVLASGKRAWYFYGASANSHRERMPSYLLQWEAIRWARARGCLEYDLWGVPDAS
                     EEVLEANFMARSDGLWGVYRFKRGFGGQLRRSIGPWDRVYSPVMYNLYSRVAGARVGF
                     G"
     gene            2462..3652
                     /locus_tag="AC16V7_08245"
     CDS             2462..3652
                     /locus_tag="AC16V7_08245"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase FemX"
                     /protein_id="HIR6257150.1"
                     /translation="MDHSIVNGAGWNTLIASFPDPHLLQTWEWAQVKSRFGWNPLYMV
                     WKHPGEAGAVIRDLTASTLGGQIPFAAALVLQRNIPIRMFSGNLRVLYVPKGPLMQDW
                     GNSAHRDRVFQDLKELGRKQGAIFLKIDPDIRLGKGIPGEDGAQESELGKKIANSLRH
                     QGWVFSSEQVQFRNTLLVDLKPSEEMLLAGMKQKTRYNVRLAMRKGVSVRIGTQDDLG
                     LLYRMYAETSVRDRFVIRDESYYRFLWQTFMSGDRSPDISDGPRALPLIAEVSGNPVA
                     ALVLFYFVHKAWYMFGMSREAHREKMPNHLLQWEAMRRAKLAGCTTYDLWGAPDEFVQ
                     NDPLWGVYRFKEGLGGVVVRHLGAWDLPIRPVIYKLYTELLPRILAIMRRRGKFRTHR
                     MVSI"
     gene            3671..>4466
                     /locus_tag="AC16V7_08250"
     CDS             3671..>4466
                     /locus_tag="AC16V7_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007192654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-aminocyclopropane-1-carboxylate
                     deaminase/D-cysteine desulfhydrase"
                     /protein_id="HIR6257151.1"
                     /translation="MINLPRVKIAYLPTPAHAMPRLSAALSGPRLLIKRDDQTGLAFG
                     GNKTRKLELLMAEAQANGAKTLVTAGAVQSNHCRQTAAAAARFGMDCILVLAGEKPIE
                     PSGNLLLDHLFGAELIWTQRDQRDRRVQEVFQNAWEEGRRPYLVPYGGSSPTGAAAYA
                     LAIEELIEQCQTFELEGHSPDWIVFPSSSGGTQAGLVAGARLFGYGGKILGISVDEPA
                     DRLRQNVAELAGRTAEHLGEQISFVPDEILVDDSFIGDGYGIMSEKE"
BASE COUNT         1087 a          959 c         1246 g         1174 t
ORIGIN      
        1 ctggaaaaca aaagcaacaa atccatggtg tttcggtggt gggccgcctg gatgaactgg
       61 cgcgggtaat tagcgtccgc catgtggacg aagtggtgat cgcaattccc agtgcgcctg
      121 gtagtgtgat ccggcaagtt gcagatgtat gccgtttgaa gagtttgccg ttccgaacaa
      181 tgcctggaat atttgaatta atcggtggta ccgtcagcgt tagccgcttg agagaagtgg
      241 aaatcaccga tttgttgcgc agagaaccca caaagataga tgagaaaatc attggatcaa
      301 gtctgggagg gcgccgtgta atggtcaccg gtgctggggg atccattgga agggaattat
      361 gtattcaaac ggctcgctgg ggaccctctg aacttatctt attgggtcat ggcgagaaca
      421 gtatttttga gacactgatc gagatcgagg aaaatttccc atccatccct attcatccag
      481 taattgcaga tattcgcgac ctggatcgat tgtcggttgt ttttgacgat ctgcggcctc
      541 aggttatatt tcatgctgcg gcgcataaac atgtccctct catggaagca aacgttgagg
      601 aagctgtatc gaataacgtg ctcggcacac gtaatatcgt tgaggtgagt caaaattttg
      661 gtattgagcg tttggtgatg atctcaactg ataaggccat tcgaccaacc agtgtcatgg
      721 gagccaccaa acgattagca gagctgattg tactagatgc cgctattcgc actcatcagg
      781 ctttttctgt ggtgcgcttt ggaaatgtgt tgggaagccg agggagcgtc ataccgaaat
      841 tcaaacgcca gattgccaag ggtgggccgg tgaccattac ccacccagat atggaacgct
      901 actttatgac aatccctgaa gccgtgcatc ttgtattaca agcttcatct atgggcgagg
      961 ggggagaggt gtttgtgcta aatatgggtc agcaggtgcg catcctagac ttggctgagg
     1021 atttggtgcg tctttctggc ctcgagcctt acaaggatat tgatatcata tttacaggaa
     1081 tccgtcctgg agaaaagctt agtgaagatc tatgggatca atgggttcaa tttgagccca
     1141 cttcacaccc tgatatcctc ctattaaatg atgaagacat cattagtggg caagaattgc
     1201 gtctcaaggt taacgatttg ctccatcttg cccgtgaagg ggattctact gctatcataa
     1261 gcattttaga cgagtttata cctggggctg ccgttcgcag cacacctcca ccagacatga
     1321 cgtccgttgt atagttgact aaaaagtgtc tcaatagcat ggacagaggt gatttgcagt
     1381 gatacagtca ctcaaaatta cccccaaaga ccattattga gatgagtatc agtttatgcc
     1441 cgaagtttca gcaagtgaat gggataaatt tctatcctct tataaggatg tccacatcct
     1501 gcaaacctcc aattggggcg agcttaaaaa agccttcggc tggcaggtag tacgctttgt
     1561 gtgtacagct ccatcccaat gtggggcaca gattttgttt cgccgattgc cttttggcat
     1621 ttcgcttgcg tatatcccaa aaggtcctgt ggggaaagag tggagcgcgt tatggtctga
     1681 gattgattcc gtttgcctcc agagaaaggc tatattcttg aaggttgagc ctgatctttg
     1741 ggatggtcaa gcgctggata atgtgcaagg agatgctccc gcgggatttc aggtaggtgt
     1801 gcaaactata caaccaccac gcaccctggt agtaaatatt gatggcgatg aagcgcaagt
     1861 tatggcacgc atgaagcaga agacacgcta caatatccgc ctttcccata agaagggcgt
     1921 ggtagtacgt ccttctgcgg atttacagct ttttagcacg ctgatgcgta taactgcgga
     1981 acgcgatcgc tttggcgcac atagccaggc atattatgag aaagtctacg atctgtttca
     2041 tcctcgcgga gagtgtgaac tgctgctggc agaatatgag ggcgaaccct taggtgcatt
     2101 gatggtacta gccagtggca agcgagcctg gtacttttac ggcgcttctg caaatagcca
     2161 tcgagagcga atgccatctt acttattaca atgggaagcg attcgatggg cgcgtgcccg
     2221 cggttgcctt gagtatgatc tctggggtgt gccggatgct tccgaggagg tgctggaggc
     2281 gaattttatg gcccgttcgg atggtttatg gggcgtttat cgcttcaagc gtggttttgg
     2341 aggtcaattg cgccgctcca taggtccctg ggatcgggta tattcacccg tgatgtacaa
     2401 tctttattct cgggtagctg gggctagagt gggttttggc tagagaagag agcaacagat
     2461 cttggaccat tctatcgtca atggagctgg ctggaataca ctaatcgcat ctttccccga
     2521 tcctcatttg ttgcagacct gggaatgggc gcaagttaaa tcccgatttg gttggaatcc
     2581 gctatatatg gtgtggaaac atccaggaga agcgggagcg gtcatcaggg atctgactgc
     2641 atctaccctt ggagggcaga taccgtttgc ggcagcactg gttctgcaac gcaatatccc
     2701 aatccgcatg ttttcgggca atttgcgagt gctctatgtc cccaaagggc ctttgatgca
     2761 agactgggga aattctgccc atcgggaccg cgtattccag gatttgaaag agctaggtcg
     2821 caagcaaggg gctattttcc tcaaaatcga tcctgacata cgtctcggca aagggatccc
     2881 aggggaagat ggagcacaag aatctgaatt aggaaaaaag attgctaact ccctcaggca
     2941 tcaaggttgg gttttttcta gtgagcaagt gcaatttcgc aatacacttt tggtagatct
     3001 caaaccttct gaggagatgt tgttggcggg gatgaaacag aagacacgct acaatgtccg
     3061 ccttgcgatg cgaaaaggtg tgtctgtgcg cataggcacc caggatgatt tgggactttt
     3121 ataccgcatg tatgcagaga catcggtgcg ggatcgcttt gtgattcgag atgagagtta
     3181 ttaccgattc ctctggcaaa catttatgtc tggtgaccgg tccccagata ttagcgatgg
     3241 acctcgcgcg ttgccactca tcgcggaagt gtctggcaat ccagtagccg cattggtatt
     3301 gttttatttt gtgcataagg catggtatat gttcggtatg tcgcgtgaag cgcatcgcga
     3361 aaaaatgccc aaccacttgc tgcaatggga agccatgcgc cgtgcaaaac ttgcaggctg
     3421 cactacgtat gatttatggg gtgcgcctga tgagtttgtt caaaacgatc ctttgtgggg
     3481 tgtgtatcgc tttaaagagg gattaggggg agtagtagtt cgacatcttg gcgcttggga
     3541 tttgcccatt aggccagtta tctacaagct atacaccgaa ttactgcccc gtatattggc
     3601 gatcatgagg cgtagaggca agtttcggac tcacaggatg gtttcaatct agaaatcaaa
     3661 ggtggagaga atgattaatt tgcccagagt taagatagcc tatctgccca ctcctgctca
     3721 cgccatgccg cgtttatcag ctgcgttaag tgggccgagg ttgctcatca agcgcgatga
     3781 tcaaacgggt ctggctttcg gaggtaataa aacccgaaag ctcgagttac tgatggcaga
     3841 ggcacaggcc aatggtgcca aaacgctggt tactgctggc gccgtgcagt caaatcactg
     3901 tcgccagacg gcagcggctg cagctcgctt tggaatggat tgtattcttg ttttggcggg
     3961 ggaaaagccg attgaacctt ctggcaatct tttgctcgat catctgtttg gggctgaatt
     4021 aatatggact cagcgcgatc aacgtgatcg gcgtgtgcag gaagtgttcc aaaatgcctg
     4081 ggaagagggg cgcaggcctt acctggttcc atatggtgga tccagcccaa cgggtgcagc
     4141 cgcttatgcg cttgcaattg aggagttgat cgaacaatgc cagacctttg agcttgaagg
     4201 tcattctccg gattggattg tttttccctc gtcttccgga gggacacagg ctggattagt
     4261 agctggagca cgtctgtttg gatacggggg gaaaatcctg gggatcagcg tggatgagcc
     4321 agccgatagg ctgagacaaa atgtggctga gttagctggt cgaacagctg agcatttagg
     4381 agagcagata tccttcgtgc ctgacgagat tcttgtagat gatagcttca taggcgatgg
     4441 ttacggcatc atgagcgaga aagagc
//
LOCUS       DBNHOH010000474         9863 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_989639, whole genome shotgun sequence.
ACCESSION   DBNHOH010000474 DBNHOH010000000
VERSION     DBNHOH010000474.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9863)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9863)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9863
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_989639"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /gene="mscL"
                     /locus_tag="AC16V7_08255"
     CDS             <1..381
                     /gene="mscL"
                     /locus_tag="AC16V7_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003446703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HIR6257152.1"
                     /translation="IIIGAAFGKIITSFVNDVLMPPIGLLLGGVNFANFFLTLSGPTV
                     KTLEEAQAAGAVTLNYGVFINTIIDFLIVAFAIFLLIRQVNRLQRQPAPEPAAPTTKE
                     CPYCFTSISIKAVRCPNCTSQLEE"
     gene            441..3209
                     /gene="alaS"
                     /locus_tag="AC16V7_08260"
     CDS             441..3209
                     /gene="alaS"
                     /locus_tag="AC16V7_08260"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIR6257153.1"
                     /translation="MTNSLSGSKIRQEFIDYFVNKAGHTFVPSSSLVPGGDQTLLFTN
                     AGMVQFKDVFLGTDKRPYTRAANSQKCMRVAGKHNDLDDVGRDDTHHTFFEMLGNWSF
                     GDYYKKEAIAWAWELLTDIWGLPRQQLWVTCFRDDIGDIPQDDEAASEWTKQPGMDPS
                     HVLFFGRKENFWEMAEVGPCGPCSEIHIDRGPQYCNKQAVPGHVCRVNGDCTRFLELW
                     NLVFIQYNRHSPTMLEPLPAMHVDTGMGLERIVSVLQDVDSNYKTDLLNPMMDAVQRL
                     TGHNQAERRANVTPYRVIADHSRAAAFLIADGVVPGNVGRNYVCRMIVRRAARFGSKI
                     GLDEPFLAKVAEVVIDTYGDFFPELNRNRTSILDNLTREEIRFQRTVEGGVTKLENLL
                     ARLKAQGGIVLSGDQAFELYATYGLPLEITRDIAREQNLEVDEPAFRRAMEEHRLASG
                     AGETFGPLGGEDVDIYRKLLEELQGQGKLGDSGVAYDPYSTVEVEGPVLALVKEGQII
                     SSASQGDKVEIVLPETCFYVEAGGQVSDTGSIVAAGKPDPSETRRSMWKIHISEMYKP
                     AAGMIVHVGEVLQGEVKTGDQAIARVDVQRRHDIMRNHTATHLLHHELRAVLGEHARQ
                     AGSLVAPDRLRFDFTHPEALTTQQLEQIEAGVNRNILSNYRLSIAFKPLQQAIAEGAI
                     ALFGEKYAETVRNITIGDAEPFSNELCGGTHVDETGDIGTFLITNEGSAAAGIRRIEA
                     VTGRGAYNLIQRRFRALKQSAALLAAPPEETPTKTQALLDELENARKQVAALRSELAA
                     SDFAQHLEDVPLIVDVPVLVAKLPGADVDTLRLMTDRFRERYPSGVAVLASIIEGRPI
                     LVAAVTQDLIERGLHAGELIKFVAQPLGGGGGGRPTLAQAGGKDASQLDKALGSVAGW
                     VAEKLGAD"
     gene            3398..5440
                     /locus_tag="AC16V7_08265"
     CDS             3398..5440
                     /locus_tag="AC16V7_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257154.1"
                     /translation="MMKNWHFRSNTVPIALFAYCLISFGLLIPWLGFYWDDWPSIYYL
                     HVLGPRGFIDAFAVDRPTLGWFFMLTSSIVGESMLAWQIFGLLSRWLSCLALWWMLRV
                     LWPNHDRQVTWTALLFAAYPGFLQQYIAVTYSHTWLVLATFLLSMGSMIWAIRKPRYF
                     WPLILFSCILSAWCMFTDEYFFGLELLRPIILWLVVQPKGNGLTKRLTSVTIRWLPFV
                     AVIAAFLIWRLFIHVSPRGQVRIFDKISESPIIAIREIVTTVAQDIVESSLIAWGQVL
                     NIPRQTSYGMGPTVLYLFVTALSIALAMIYLSRFGLQTASGSPPGGTRYWSRQAIFLG
                     LAGLLFAGIPFWVTNLPIELRFPWDRFTLAFNLGASLLLAGVLELLPKRRILNVLILA
                     TLLGLASGMHEQLANLYRREWNSQKALFWQLVWRAPGIQPGTMLLSAELPFIYYSDNS
                     LSAPLNYTYNPAQPSSELSYMFYNIESRLGSKLEGFEKGQEVNETYRALYFSGTTSQA
                     LAIYYSPPGCAKVIDPATDAKTPQKPLYFSRVLSLSDLGFIQATATPPAMPPKAYFGD
                     EPEPNWCFYFEKADLARQQSDWAEVVRLGEQAFQLNHRLYEVNATELLPYIEGYARTG
                     RWEKARELTNEAHQLTFRMNSILCETWKRIQGQDGLPLEGQNTVNRVKQRLKCATN"
     gene            5518..6483
                     /locus_tag="AC16V7_08270"
     CDS             5518..6483
                     /locus_tag="AC16V7_08270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR6257155.1"
                     /translation="MKNLSIVIPVYNSESTLGDLVERLAKVLPSLAPVYEVILVNDGS
                     RDRSWEIIDQLIQTYSWASGINLMRNYGQHNALLCGVRQARYEIIVTMDDDLQHPPEE
                     IHKLLDVLSQGNDVVYGIPHKQPQNWWRNIFSMIIRRLLAYVMGVKNIRDFASFRAFR
                     AYLRGAFADYQNPNVVLDVLLAWGTTRFANAVVTEMPRTVGRSNYNFFKLVQYTMIVL
                     TGFSTVPLRITSMLGFIFTFVGIAIFLYVLAIYFLVGSIPGFPFLASIISIFSGTQLF
                     ALGIIGEYLARIFDRSMDRPAYVVRESIRHGEGYITGIEDKVEAA"
     gene            6480..7175
                     /locus_tag="AC16V7_08275"
     CDS             6480..7175
                     /locus_tag="AC16V7_08275"
                     /inference="COORDINATES: protein motif:HMM:NF020463.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HIR6257156.1"
                     /translation="MKCVLLQPSYIPWRGYFHQIHKADIFVFYDDVQFDKHGWRNRNR
                     IKTHQGTIWLTIPVQSHTAPLHHTPINEVIIDNHQPWNRKHWMTLSQSYGKAPFFSRY
                     ADLLEAYLRQPQELLAEFTIELTIALSKELGIMDTQFVRSSSLTVTGTKTERLIQIVN
                     CLGADHYITGPAAKSYIDQSQFDLAGIRLEYMEYNYPDYEQLYPPFDPQVSILDLMFM
                     TGPNALDYIVGKG"
     gene            7179..8324
                     /gene="rffA"
                     /locus_tag="AC16V7_08280"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             7179..8324
                     /gene="rffA"
                     /locus_tag="AC16V7_08280"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose:2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HIR6257157.1"
                     /translation="MDKEIFIDFNRPALVGNELAYITEAVSRLHLSGDGYYTHLCQDI
                     LAEELGARKVLLTTSCTHALEMAALLLDVQPGDQVIVPSFTFVSTINAFVLRGAQPVF
                     IDIRPDTLNLDENQLEGLVNERTKAILPVHYAGVGCEMDDILKIAEKYNIPVVEDNAH
                     GLFGKYKEKLLGTFGCMATLSFHETKNISCGEGGALLINELEYIERAEIIREKGTNRS
                     RFFRGQVDKYTWVDIGSSYLPSDILAAFLYAQLEKRLEIQALRKAIWDRYYEGLASWA
                     EQFNVRLPIVPQHCEQPYHMFYLLLPSLEIRQSLISYLKHRGIQSVFHYQPLHLSEMG
                     RRLGGIPGDCPVTEEISDQLLRLPFHAHLEEADQMRVIQAITSYQQK"
     gene            8337..8555
                     /locus_tag="AC16V7_08285"
     CDS             8337..8555
                     /locus_tag="AC16V7_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257158.1"
                     /translation="MSIMSILSPLVEIVTAPAKANPAYLDPGSGSFILQLLLAALVGS
                     AFIIKSYWKKITDFFRKLFSRADQRKEE"
     gene            8552..>9863
                     /locus_tag="AC16V7_08290"
     CDS             8552..>9863
                     /locus_tag="AC16V7_08290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6257159.1"
                     /translation="MTSANFQGLTRSVEASFRDPSGHLFTLDGTLFRQVNRFYQEHYT
                     HLMDSGLYQSLVKDGLLIPHDEVSVTPPDPSLAWKVIQPETIPFISYPYEWSFSQLKD
                     AAQVTLEIQKRALEHGMVLKDSSAYNIQYHQGKPVLIDTLSFEIYTEGQPWIAYRQFC
                     QHFLAPLSLMSHRDIRMGQLLRIYIDGVPLDLASRLLPWKTRFNWPLQMHIHLHAASQ
                     KRYASSSLPSERKMGKTAMLGWIDSLESAIRGLSWSPEGTAWSEYYTDTNYTSSGLEH
                     KKALVREYLDFFHPKSIWDLGANTGLFSRIASQMGIQTTAFDIDPGAVELNYRECRKE
                     SETNLLPLLLDLTNPSPGIGWENRERQAFAERGPGDAVMALALIHHLAIANNVPLQRL
                     ASFLHGLSQWLLIEFIPKGDSQVQRLLASREDIFTEYTQANFEQV"
BASE COUNT         2425 a         2548 c         2429 g         2461 t
ORIGIN      
        1 atcattatcg gcgcggcttt cggtaaaatc attacttcgt ttgtgaacga tgtccttatg
       61 ccccccattg ggttattgct gggcggggta aattttgcta acttttttct aaccctatcc
      121 ggtcctacag ttaaaacact ggaggaggcc caggcggcag gagcagtgac gctcaattat
      181 ggtgtattta tcaatacaat catcgatttt ctcatcgtcg cttttgccat ctttttgctg
      241 atacgccagg tcaatcgctt gcaacgccaa ccagccccag aacctgccgc tcccaccaca
      301 aaagagtgtc catattgttt cacgtctatt tcgatcaagg cggtacgctg cccgaattgc
      361 acttcgcaat tagaagaata acgaattggc tatcaagaat taagattaaa atatctatta
      421 aaaagaacat ttaggatggt atgaccaact cattatctgg atccaagatt cgacaagaat
      481 ttatcgatta cttcgtcaat aaagccgggc acacatttgt gccgtcctcc tcacttgtcc
      541 cgggtgggga tcagacgctc cttttcacca acgcaggtat ggtgcaattt aaggatgtct
      601 tcctcggcac cgacaagcga ccatacacac gagctgccaa ttcacaaaag tgcatgcgcg
      661 tagctggcaa gcataatgat ctcgacgatg tcggtcgaga cgacacgcat cacacgttct
      721 tcgaaatgct ggggaattgg tcctttggag actattacaa gaaagaggct atcgcttggg
      781 cttgggaatt gctcaccgat atttgggggc tgccgcgcca gcagctttgg gtaacctgtt
      841 ttcgtgacga tataggagat atcccacaag acgatgaggc tgccagtgag tggaccaagc
      901 aaccgggcat ggacccgtct catgtgctct ttttcgggcg caaggaaaat ttctgggaga
      961 tggccgaagt gggcccctgc ggtccatgca gcgaaatcca catagaccgc ggtccacaat
     1021 attgcaataa acaagctgtc cccggccacg tctgtcgagt caacggtgac tgcacccgtt
     1081 tcttggagct gtggaacctg gtgttcatcc agtataaccg ccatagcccc acgatgctgg
     1141 aaccactgcc cgctatgcac gtcgataccg gcatggggct ggagcgtatc gtttcggtgc
     1201 tccaggatgt cgactccaac tataaaaccg atctactcaa tccaatgatg gatgcggtac
     1261 agcgcttaac agggcataac caggctgaga gacgagctaa tgtcactcct taccgtgtta
     1321 tcgctgacca ttcccgtgca gctgcattcc ttatcgctga tggcgtcgta ccaggaaatg
     1381 taggacgcaa ctacgtatgt cgtatgatcg tgcgccgtgc tgcccgcttc ggcagtaaaa
     1441 ttgggttgga cgaaccgttt ctcgccaagg tggctgaagt ggtcatcgac acctacggcg
     1501 acttttttcc cgagttgaat cgaaaccgca caagtatcct ggacaactta acccgcgagg
     1561 agatacgctt ccaaaggact gtagaaggtg gtgtgaccaa actagaaaac cttctcgctc
     1621 gcctgaaggc acagggggga attgtcttat cgggtgacca ggcatttgaa ttgtacgcta
     1681 cctatggcct gccgcttgag atcactcgcg atatcgcccg ggaacaaaat ctcgaggtag
     1741 atgaacccgc ttttcgcagg gcaatggagg agcaccgcct cgcttctggc gcaggtgaaa
     1801 ccttcggtcc cctgggtggg gaggatgtgg atatttaccg caagctcctg gaagagctac
     1861 aaggacaagg caagcttggg gatagcgggg tggcatatga tccttatagt accgttgagg
     1921 tggaaggtcc agtccttgct ctggtcaaag aagggcaaat tatttcgagc gcttcgcagg
     1981 gagataaggt agagattgta ctgccggaaa cctgtttcta tgtcgaggct ggtggtcaag
     2041 tctccgatac tggttcaatc gtcgcggcgg gcaaacccga tccttccgag acccgtagat
     2101 cgatgtggaa gatacatatt tccgaaatgt ataaaccagc cgcaggaatg atcgtgcatg
     2161 tgggcgaggt gctacaaggc gaggtgaaga cgggtgatca agctatcgcc cgcgtggatg
     2221 tccaaagacg ccacgatatt atgcgtaacc acactgccac ccacttgctc catcacgaat
     2281 tgcgcgctgt acttggtgag cacgctcgcc aagctggttc cctggtagct ccggataggc
     2341 tgcgctttga cttcacccat cctgaagcgc ttaccactca acaacttgag caaatcgagg
     2401 ctggggtaaa ccgcaatata ttgagcaact ataggttaag tattgcattc aagccattgc
     2461 agcaggctat cgctgaaggc gccattgcac tttttggtga aaaatacgct gagacggttc
     2521 gcaacatcac cattggcgat gcagaaccat tttccaacga gctatgtgga ggtacgcacg
     2581 tcgacgagac aggcgacatt ggcacttttt tgatcaccaa cgaaggcagc gcagctgcgg
     2641 gcatccgccg catagaggct gtgactgggc gcggcgcata caacctcatt cagcgtcgct
     2701 ttcgcgcgtt aaaacaatct gcagctttgc tggctgcgcc ccctgaagag accccaacta
     2761 agacccaagc cttgctggac gaactggaaa acgctcggaa gcaagtcgct gctttgcgaa
     2821 gcgagctagc cgcaagcgat ttcgcccaac acttagagga tgtccctttg atcgtcgacg
     2881 ttccagtgct ggttgccaaa ctaccggggg cagacgtcga tacgctgcga ttgatgaccg
     2941 atcgcttcag ggagcgatat cctagcggcg tggctgtgct ggcatcgatt attgaaggac
     3001 gtccaatttt ggtggcggca gttacacaag atctcattga aagaggcctc cacgctggtg
     3061 agctgatcaa gtttgttgcc cagccattgg gtggcggagg cggaggccgt cctactctag
     3121 ctcaggcagg cggaaaagac gcatctcagc tggataaagc cctgggtagc gtagctggct
     3181 gggttgctga gaagctaggc gctgattagc ctggagacaa ctctccatca aaaaagcaat
     3241 ttattcccaa ataatgggta ctcgggtaga aatcggatcc caaccccagt ctcttgaacc
     3301 gtctctctga atgtaaagag gggggtgaga caattcaatg cccttggggg agggcattac
     3361 ccttttacgt tataattcca tcaaataagt cccagcgatg atgaaaaact ggcatttccg
     3421 ttcgaacaca gtccctatcg cacttttcgc ctattgcctg attagctttg ggctactcat
     3481 accttggttg ggtttttatt gggatgattg gccttcgatt tattatcttc atgtgctcgg
     3541 cccaaggggg tttatcgatg cgttcgcagt agatcgccca actctgggct ggtttttcat
     3601 gctcacttcg tcaattgtgg gggaatcgat gcttgcctgg caaattttcg gtctgctctc
     3661 gcgctggctg agctgccttg cactgtggtg gatgctaagg gtgctctggc ctaaccatga
     3721 ccgccaagtg acctggaccg cgctcctgtt tgccgcttat cctggcttcc tccaacagta
     3781 cattgccgtc acgtacagtc acacctggct ggtcctagcg acctttctcc tttcgatggg
     3841 gagcatgatt tgggcgattc gcaagccgag gtacttttgg ccgctgattt tgttctcctg
     3901 cattctttct gcctggtgca tgttcaccga cgaatacttc ttcgggctcg aactgctacg
     3961 cccaattatt ctttggttgg tggtccaacc gaagggcaac ggtcttacca aacgcctaac
     4021 tagtgtaaca attcgctggc tgccatttgt cgcggtgatt gcggcatttc tgatttggcg
     4081 cttgttcatc cacgtctctc cccgcggcca ggtacgcatt ttcgataaaa taagcgaatc
     4141 gcccataatt gccatacgag agatcgtaac aacagtggcg caagacatcg tcgaatcaag
     4201 cctaatagcg tggggccagg tactcaacat tccaagacaa actagttacg gcatgggacc
     4261 aaccgttctc tacctttttg tcacagccct gtcgatcgca ctggcaatga tatatctatc
     4321 ccggttcggt ctgcagacgg caagcggctc accgcctgga ggaacccggt attggtctcg
     4381 ccaagccata ttccttggtc tggcaggttt actgtttgca ggcatcccat tttgggtaac
     4441 aaatctaccg atcgagctgc gttttccatg ggatcgcttc accctagcct ttaacctggg
     4501 cgccagcctt ctcctcgccg gggtcttgga gctcctgccc aaacgccgaa tcttaaatgt
     4561 gctgatcctc gcaacgcttc tgggtctggc aagcggaatg cacgaacagc ttgccaatct
     4621 ctaccgtcgg gaatggaatt cacagaaagc cttattctgg caacttgtct ggcgagcacc
     4681 tggtatccag cctggaacaa tgcttctctc cgccgaattg ccatttattt attacagcga
     4741 taattcatta tctgcaccct tgaattacac ctataatcct gcgcaaccat cgtcagagct
     4801 atcctacatg ttttataata tcgaatctcg cttggggagc aagctggaag gcttcgaaaa
     4861 aggtcaagag gtaaacgaaa cttatcgggc actatatttt agtggaacaa catcccaagc
     4921 tttggcaatc tactatagcc cacccggttg tgcaaaggtt atcgatccag ctacggatgc
     4981 caaaactccg caaaaaccgc tatatttttc gagggtattg tcgttatctg atcttggatt
     5041 tatccaagcc acagctaccc cgcccgcgat gcccccaaaa gcatactttg gagatgaacc
     5101 agagccgaac tggtgttttt attttgagaa agcagatcta gctcgccaac aaagtgattg
     5161 ggctgaggtg gtacgcctgg gtgagcaagc attccaattg aaccaccgcc tgtatgaggt
     5221 caatgcaacc gaactgcttc cttacatcga aggctacgcc cggaccggga gatgggaaaa
     5281 ggcaagagaa ttaactaatg aagcgcacca gttaacattc cgcatgaata gcatcctatg
     5341 cgagacctgg aaaaggattc aaggacaaga tgggctgccg ctcgaggggc agaatactgt
     5401 taaccgtgta aagcagaggc tgaaatgcgc caccaattga tcaaattctc ccacttgaat
     5461 ttaaggagta cctagtcgcg tagcctcggt cagtgattat gttgcctgaa atcgcctatg
     5521 aagaatttgt caatcgttat cccggtttat aacagcgaga gcacccttgg agacttggta
     5581 gagcgcctag ctaaagttct accatcgctg gcacccgtgt acgaggtcat ccttgtcaat
     5641 gatggaagcc gcgatcgctc ctgggagatc atcgatcagt tgatacaaac ctattcatgg
     5701 gcgtctggta tcaacttgat gcgtaattat ggacaacaca acgctctctt gtgcggggtg
     5761 cgccaagctc gttatgaaat tatcgtcacc atggatgatg acctgcagca cccccctgag
     5821 gagatccaca aattgctcga cgtattatcc cagggtaacg acgttgtata cggcataccc
     5881 cacaagcaac cgcaaaattg gtggcggaac atcttctcga tgatcatccg ccgcttgttg
     5941 gcttacgtga tgggcgtcaa gaatatacgt gatttcgcca gtttccgggc tttccgtgct
     6001 tatttgcgcg gagcttttgc tgactatcaa aatccaaatg tcgttctgga cgtgcttctt
     6061 gcatggggca cgacccgctt cgccaatgcc gtagtcacag aaatgccacg cacagttgga
     6121 cgctcaaatt acaacttttt caaattagtg caatatacga tgatcgtgct aaccggattt
     6181 agcaccgtac ctctacgcat caccagtatg ttaggtttca tttttacttt tgtcggaatc
     6241 gctatcttcc tgtacgtcct ggcaatctat ttcttggttg gcagtattcc aggattccca
     6301 ttcctggcgt ccattatatc gatatttagt ggcacacagt tattcgccct ggggattatc
     6361 ggcgaatacc tggcacgcat cttcgaccgc agcatggatc gcccggctta tgtggtcaga
     6421 gaatcgatac gccatgggga aggttacatc acgggaatcg aggacaaggt cgaggctgca
     6481 tgaagtgtgt cctactgcag ccatcttata tcccctggcg tggctatttt caccagatac
     6541 ataaagccga tatctttgta ttttacgatg atgtccaatt cgacaaacat ggctggcgta
     6601 atcgcaaccg gatcaagact caccaaggaa ctatatggtt aacgatcccc gtccagagcc
     6661 ataccgctcc gctccatcat acaccgatca atgaagtaat tattgataat caccaaccct
     6721 ggaatcgcaa gcattggatg accttatcac aatcttacgg gaaagcacct tttttctcgc
     6781 gttatgccga tctacttgag gcatatctcc gccaacccca ggaattgctg gctgaattca
     6841 ccatcgagct aaccattgcc ctatctaaag agctgggaat tatggatact cagtttgttc
     6901 gttcatcttc gcttacagtg accggcacca aaactgagcg gctcatccaa attgtgaact
     6961 gcctgggagc agaccactat ataacaggtc cggctgctaa aagctatatt gatcaatccc
     7021 aatttgactt ggcagggatt agattagaat atatggagta caattacccc gattacgaac
     7081 agctttaccc cccttttgac ccacaggtat ctattttgga cttgatgttt atgaccggcc
     7141 cgaatgcgtt agattacatt gttggcaaag gataatcatt ggataaagag attttcatcg
     7201 atttcaatcg cccagctctg gtcggcaacg aactggcgta tatcactgaa gcggttagca
     7261 ggctgcacct ctccggcgat ggatactaca cacacctctg ccaagatatc cttgcggaag
     7321 aattaggtgc acgaaaagtt ttgctgacta cctcctgcac acacgccctg gagatggctg
     7381 ccctgttgtt agatgtccaa ccaggggatc aagtaatcgt accctccttc acctttgtgt
     7441 ccacgataaa tgccttcgta ctgcgcggcg ctcagccagt gtttatcgat attcgtccag
     7501 atacgctcaa cctggatgaa aaccagctcg aggggctggt caatgaacgc accaaagcga
     7561 tcctccctgt gcattatgcg ggtgtgggct gcgagatgga cgatattttg aaaatcgccg
     7621 aaaaatacaa catcccggtg gtcgaagata acgcccacgg tttgttcgga aagtataagg
     7681 aaaaactcct ggggaccttt ggatgcatgg ccacgttgag cttccacgag acaaaaaata
     7741 tctcctgcgg agaaggaggc gccctgctca taaacgagct tgaatacatt gagcgggctg
     7801 agatcatccg cgaaaagggc actaatcgca gccgtttctt ccgcgggcag gtagataaat
     7861 acacctgggt cgatattggc tcgagctatt tgccttccga tatcctggca gcttttcttt
     7921 acgcccagtt ggaaaagcgt ctggaaatcc aagctctccg caaggccatc tgggacaggt
     7981 attacgaagg gctggcaagc tgggcagaac aattcaatgt gcgattgcca atcgtcccac
     8041 aacactgcga gcagccttat cacatgtttt acttgctgct accctccctt gaaattcgcc
     8101 agtcactgat ctcatactta aaacatagag gcatccaaag cgtatttcat tatcaacccc
     8161 ttcacctatc cgaaatgggt agacgcctcg gagggattcc aggtgattgc cctgttaccg
     8221 aggaaatcag cgaccaatta ttgcgcctgc catttcacgc tcatctggaa gaagcagacc
     8281 agatgagagt aatccaggcg attacatcct atcaacaaaa gtgagtcaaa gaaaatatgt
     8341 caatcatgtc aatcctttcg cccttggtag aaatagttac tgcgcccgcc aaagcaaatc
     8401 cagcatatct ggatcctggc tcaggcagct ttatcttgca actgctgctt gcagcgctgg
     8461 ttggaagcgc cttcatcatc aaatcgtatt ggaaaaagat caccgacttc ttccggaagt
     8521 tattctcgcg agcagatcaa agaaaagaag aatgacctca gccaatttcc aaggccttac
     8581 ccgctcggtt gaagcctctt ttcgtgatcc gagcgggcac ctgttcactt tggacggcac
     8641 cctctttcgc caagtgaacc gtttctatca ggagcactat acccacctga tggattctgg
     8701 tctttatcaa agcctggtga aagacggatt gctcatcccc cacgatgagg tgagtgttac
     8761 cccgccagac ccaagcttag cctggaaagt tatccagcct gagacaattc cgttcatttc
     8821 ttacccctat gagtggagtt tcagccagct caaagacgca gctcaggtta cccttgagat
     8881 tcagaaacgc gctcttgagc atggcatggt gctcaaagac agctccgcat acaatattca
     8941 atatcatcag ggtaagccgg ttttgatcga tacactctcc ttcgaaatct acactgaggg
     9001 gcaaccttgg atcgcctacc gtcaattttg tcagcacttc ctggcaccgc ttagcctgat
     9061 gtcccaccgt gatattcgta tgggtcaatt actacggata tatatcgatg gagtaccgct
     9121 agacctggct agccggttgc ttccctggaa gacgcgcttt aactggccat tgcaaatgca
     9181 catccatctg cacgctgcat cgcagaagcg ctatgcctcc agcagcttgc ctagcgagcg
     9241 gaagatggga aaaactgcca tgctgggctg gattgacagc ttggaatcgg ctatccgcgg
     9301 gttgagttgg tcgccagagg gtactgcttg gagcgagtac tacaccgata ccaactatac
     9361 ctccagcggg ctggagcaca aaaaagcgct tgtgcgcgaa tatctggatt ttttccaccc
     9421 gaaaagcatt tgggacttgg gagccaatac cgggctattt agccgtattg ccagccaaat
     9481 gggaatccag acaactgctt tcgacatcga cccaggggca gtggagttga attaccggga
     9541 atgccgcaaa gagtcggaaa caaacctgct gccgctgctg cttgacttga ccaacccgag
     9601 tccagggatt ggctgggaaa accgagaacg gcaggccttc gctgaacgcg gtccgggtga
     9661 cgcagtaatg gctctggctt tgatccacca tctagcaatc gccaataatg tccctttgca
     9721 gcgtctggct tcattcctgc acggattaag ccaatggttg ttgattgaat ttattcccaa
     9781 gggtgattcc caggtacagc gcttgcttgc ctctcgagag gatatcttta ccgagtacac
     9841 gcaggctaat tttgagcagg tct
//
LOCUS       DBNHOH010000475         3079 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1116995, whole genome shotgun sequence.
ACCESSION   DBNHOH010000475 DBNHOH010000000
VERSION     DBNHOH010000475.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1116995"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..176
                     /locus_tag="AC16V7_08295"
     CDS             <1..176
                     /locus_tag="AC16V7_08295"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024639.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6257160.1"
                     /translation="PQACLMIGDTTVDMRAGKAAGAQTLGVLCGFGVESELRRAGADL
                     ILPTTSMLTDVID"
     gene            265..1245
                     /locus_tag="AC16V7_08300"
     CDS             265..1245
                     /locus_tag="AC16V7_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257161.1"
                     /translation="MVAKTRWVGVVFVLSLFLSWMPSLAQEITQPPNERRYYPQRGHW
                     VMGEFLEVYQSVPNSAEIYGYPITEAFQEQTLGRIVQYFEKARFELIPENPPELRVKI
                     TELGSYFYTTAGQELPLPANFPACLFFQETGKQVCYAFLEYFEANGGAAQFGYPLSNF
                     ETQDGLIVQYFQRARFEWHPEFPPGKRVKLTELGYRYFKKIGEDPARLLPAPWLAPGS
                     DIPQPVLSLRVRAFPLHAVLTQNAAQSIFIIVQDQNLLPVSNAQVTLIIRLPSGKEQR
                     VIVPGTTDRFGVIKYSFDYTDQPTGVAQVIVNAIYETFHKQTITSFRIWW"
     gene            complement(1315..3000)
                     /locus_tag="AC16V7_08305"
     CDS             complement(1315..3000)
                     /locus_tag="AC16V7_08305"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HIR6257162.1"
                     /translation="MRSKDEAFYFQAINLPPIGKGGGLRSGFLAGASSLLKTLFFFIL
                     GVMLVIALGGCTSTPKNTSNPSAMTQTSKPVLPANTTMPSGGSTPVAGFEDQSPAILP
                     AAQAELALLPDLTRYDIDLVIDSAAQTFSGQARVDVTNTEGQPLESLYFRLLPNGGQS
                     YGNGSLTVSDTRVIGDTADTILSLNNTLLEVKLPQVLQPGERVQVEFDFQGIVPLDFG
                     GDASPYAYGIYNKSEGVLALSGWYPILAVYDEGGWHLDPISPIGDSVFSEMALYTVRV
                     TMPSDLVLVSTGVEISRESLGATTKYLLVSGPMRDFFMIASPDFQVVSQIVDGTRVNS
                     YYFSEDEYAGAVGLSVSAESLRVFNQRFGKYPYIELDMVQAPMRNALGVEFPGIYLIG
                     ASLYDEPDTPDFAVTVAHEVAHQWWYSLVGNDVFAEPWLDEALSTYSSGIYYQDVLGD
                     GAYSGLASYWQSRYDTLIQDGEDDVITRDLKYFESLGVPRVYSGVVYRKGALFFKALR
                     EAIGDSAFFDALQGYYLDNKYGVGNGDELLSAFEQASGQELDDLYNEWLYTSK"
BASE COUNT          764 a          839 c          732 g          744 t
ORIGIN      
        1 ccccgcaagc atgcttaatg attggagata cgacggtgga tatgcgcgct ggaaaagccg
       61 ctggtgcgca gacgctaggc gtcctgtgtg gatttggggt ggagagcgaa ctccgtcgcg
      121 cgggtgcaga tttgatctta cccacaacat ctatgttgac cgacgttatt gattaaccgc
      181 cagggatggg gggtgacaaa atagcaaacc gaatacctgg catatggtat tgctatttcg
      241 caatattttg catataatca ttttatggtc gccaaaaccc gctgggtagg ggtagtattt
      301 gtgttgtcac ttttcctgag ctggatgcct tcactcgctc aggagatcac ccaacctcct
      361 aatgagaggc gctactaccc ccaacgcggg cactgggtca tgggggaatt tttagaagtt
      421 tatcagagcg tccccaactc ggcagaaatt tacggatatc caataaccga ggcattccag
      481 gaacaaaccc tggggcgcat cgtgcaatac tttgaaaaag cgcgctttga gctaattcct
      541 gagaacccgc cggagctgcg tgtcaagatt actgagctgg gcagttattt ctacacgacc
      601 gcagggcagg aacttccctt accggcaaat ttccctgcct gcctcttctt ccaggagaca
      661 ggcaagcagg tctgttatgc attcttggaa tacttcgaag ccaatggtgg ggcagctcaa
      721 ttcggttacc cactctccaa tttcgagact caagatgggc tcatcgtgca gtatttccag
      781 cgtgcccgct ttgagtggca tccagagttc cctccaggaa agcgtgtcaa actcaccgaa
      841 cttggatacc gctacttcaa gaaaattgga gaagaccccg cccgattgct gccagcacca
      901 tggttagcac cagggagcga tattccccaa ccagtgctta gcttgcgcgt acgggctttc
      961 ccgttgcacg ctgtccttac gcagaatgcc gcgcagagta ttttcatcat cgtccaggat
     1021 caaaatctgc ttcccgtctc taatgcccaa gtgactctga tcatcaggct gccctccgga
     1081 aaagagcagc gggtaattgt gcccggcact acagacagat ttggggtgat caagtattcc
     1141 tttgattaca ccgaccaacc aacaggcgtg gctcaggtga tcgtcaacgc catctacgag
     1201 acattccaca agcaaaccat cacatctttt cgaatatggt ggtaagaaca tccacagaat
     1261 gactcagccc cacagaataa tctatgggtg agttattttg atcgtttgtg ttccctattt
     1321 ggatgtatat aaccactcat tgtacagatc atccagctcc tggcctgagg cttgctcaaa
     1381 ggcagaaagc aattcatcgc cgttaccgac cccgtattta ttgtccaaat aatagccttg
     1441 taaggcatca aaaaacgctg aatcgccgat cgcctcccgt agcgccttga agaacaaggc
     1501 tccctttcta taaacaactc cactatagac acgagggaca cctaagctct caaaatattt
     1561 caaatcccgg gtgatcacat cgtcttcgcc atcttggatc agcgtgtcat agcgggattg
     1621 ccagtaagat gccagaccac tatatgcgcc atcccctagc acatcctgat aatatatccc
     1681 cgacgaatat gtgcttaggg cttcatccag ccatggctca gcaaatacgt cgttacccac
     1741 caggctgtac caccattggt gcgctacctc gtgggcaacg gtcactgcga agtcgggtgt
     1801 atccggttca tcgtatagcg atgcgccgat caggtatatc cccggaaatt ctacccccag
     1861 ggcatttcgc atcggcgcct ggaccatgtc caactctatg tatgggtact tgccaaaccg
     1921 ttgattgaac acacgtaggg attctgcgct tacagataaa cccaccgcac ctgcatattc
     1981 atcctccgag aagtaatacg aattgactcg ggtgccatcc actatttggc tcaccacctg
     2041 gaaatcggga ctggctatca tgaagaaatc ccgcattggc ccgctgacca agagatattt
     2101 tgttgtagcc ccaagacttt cacggctgat ctctacgcct gttgacacaa ggaccagatc
     2161 gcttggcatt gttacgcgaa ctgtgtagag cgccatctcg ctgaagaccg aatcacctat
     2221 aggcgaaatc ggatccaggt gccagccccc ctcatcgtat actgccagga tagggtacca
     2281 gccggagagt gccagcaccc cttcgctttt attgtagatg ccataagcat agggtgacgc
     2341 atcgcctccg aagtcaagtg gtacgatccc ctggaaatcg aactcgacct gaactcgctc
     2401 accaggctgc agtacttgtg gtaatttcac ttcgagcagc gtgttattta gcgacaggat
     2461 tgtatcggct gtatctccta tcacccgggt atctgaaacg gttagcgagc cattcccgta
     2521 gctctgacca ccattgggta acagacggaa atacaagctt tctagtggct gcccctcagt
     2581 attggtcacg tccacacgcg cttgcccact gaaggtttga gctgcggaat ctatgaccaa
     2641 atcgatgtca tagcgcgtca gatcaggtag aagagccagt tcagcctgtg ccgctgggag
     2701 tatggcgggg ctttgatctt caaacccagc tacaggtgta ctgccgccag acggcattgt
     2761 ggtattcgct ggcaggactg gtttgctcgt ctgtgtcatc gccgagggat tggatgtatt
     2821 ctttggagtg gatgtacagc ctccaagagc tatcaccaac atgaccccca agatgaaaaa
     2881 aaacagggtt tttagcaagc tcgaagcacc tgccagaaac cctgaacgca acccaccccc
     2941 cttcccaatg ggagggagat ttatggcttg aaaataaaac gcttcatctt tcgatctcat
     3001 agctatcgat gacctccccg tcacgtgaaa taaactcgaa catgatcttc ttagtggtag
     3061 ctttcacccg catcgcccc
//
LOCUS       DBNHOH010000476         3131 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_27552, whole genome shotgun sequence.
ACCESSION   DBNHOH010000476 DBNHOH010000000
VERSION     DBNHOH010000476.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_27552"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..775
                     /locus_tag="AC16V7_08310"
     CDS             275..775
                     /locus_tag="AC16V7_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257163.1"
                     /translation="MPDFPGAIPIESGDDVAQSHLDWLIDFRQAAELMQLFLSMYLND
                     AEDVCIPAGAEGINVLKAVEDSPASMSVSITRGICVAGRIPFALLADTLVGAFVAPTT
                     NPRKDIIVADPDGKQVRAITGVEDASPVKPSAPAGTLELCEIDLIVSQTSILNADITD
                     SRVLGG"
     gene            777..2612
                     /locus_tag="AC16V7_08315"
     CDS             777..2612
                     /locus_tag="AC16V7_08315"
                     /inference="COORDINATES: protein motif:HMM:NF025253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HIR6257164.1"
                     /translation="MRVLLISIVIALFAVATNAQVYHMGGAAFGNNNALQWIRGDDAL
                     APVMRINASNDVIIRAAEVSGTISFADHGSDTAQILISPSLGAVTAAGDINTGGDVNI
                     SNPAANSTVLVDLTNDARTWQLRVAGGEGDRFRIYDATGTADPFQIEAGADNDALYID
                     SAGLIGINNANPLHRLHVIESGSSGMTAFTGTDWFFESDGNSIINIGSPNGFNSALAL
                     GSVSDPNGLLLQYNPATGKAILASTDAGDAVQIWSGNSGLAVEFNTDQTASFVQEIDA
                     AADTDGNHIFGRIRLGNPAGDNAGISHYDHMSLTNYMIKQNANGLTRVNSPSGQDMEL
                     AINNTMKLEVESGGDVHIVQALDVDGNIELGDTLVGNVGNGEIPIDTATNGLALIRAN
                     TSDGSDDDYISLAGGGDVGTDRGGYLGVYGMEFTTFNLHGSIGLFAASHANSKIAMNV
                     NGTNSIIIDINSQVFMAPVYADAIGTPRRDLFIKDDGQLGYVSSTRADKEQIEDITDA
                     HLTKIWQLEPKRYERVDDPTNTKEFGLIAEDVAALGIPGLVSYARKATMVDERPGNPL
                     GPLYEYSEDLTKPETVNYSFLTTILLREVQKLRQRVTTLEGHHTR"
     gene            2624..3019
                     /locus_tag="AC16V7_08320"
     CDS             2624..3019
                     /locus_tag="AC16V7_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257165.1"
                     /translation="MKKFTSIQILLVAAMSGGILTAIFSEYPQAAAQNELGNVKDAVA
                     REIRMNPQGALDSFINRIFANTDRTITTKILEEVTLDLGIDITSKDDPHADSFIRISR
                     ALEEAGITNVGECIAAIKAVPPAWEIPQD"
BASE COUNT          851 a          640 c          839 g          801 t
ORIGIN      
        1 tcttttcgag gagaaggggg atttggagga gcagggttta tggggtacgg acgtggggga
       61 aaagggggga acgcggggga tttcaataat gcttctgccg gtggaggcgc tggggggcaa
      121 gcaaaaactg tggatgctca cgcggttacg ccgggagcat ccttaactgt gacaattgga
      181 gcgggcggta cagcaggttc tggcggcgtt ggcactccca gtgggaatga cggcaatgtt
      241 ggtgaagctg gtgccgtact agtggagtgg taaaatgcca gatttcccag gagccatacc
      301 cattgaaagc ggagacgacg ttgcgcaaag tcatttagac tggctcatcg attttcgtca
      361 agctgcagaa ttaatgcaac ttttccttag catgtatctc aacgacgcgg aggacgtatg
      421 catacctgct ggtgcagagg gtattaacgt attaaaagct gtggaagatt cccctgctag
      481 catgtcagta tccataacta gaggcatatg cgttgcgggg aggataccat tcgccctgtt
      541 agcggatacc ctggttgggg cttttgtagc tccaacaact aatcctcgaa aagacattat
      601 cgttgccgat cctgatggca aacaagtaag agcaattaca ggggtagagg atgcttctcc
      661 cgtgaagccc tccgcacctg cgggaactct ggaattatgt gagatagatc ttattgtttc
      721 acaaacatca atcttaaacg cggacattac ggattctcgg gtccttggag gttaacatgc
      781 gagtactttt aatttctatc gttattgcac tttttgccgt tgcgacgaat gctcaagtgt
      841 atcatatggg aggggcggca tttggcaata ataacgctct tcaatggata cggggagacg
      901 atgccctcgc tcctgtcatg cgaattaatg cttccaatga cgtcataatt cgtgctgcgg
      961 aggtttcggg aaccatatct ttcgcagacc acgggtcgga taccgctcaa attctaattt
     1021 ctccttcctt aggagctgtg accgccgcag gtgatataaa tacaggaggt gatgttaata
     1081 tatctaaccc tgctgcaaac agcaccgtgc ttgtggattt aacaaatgat gcccgaactt
     1141 ggcaattacg ggttgcagga ggcgaagggg ataggtttcg aatttatgat gcaacaggaa
     1201 ccgcagaccc cttccaaatt gaggcggggg cggataacga tgctttatat atagattctg
     1261 cagggttgat tggaatcaat aacgcgaatc ctctacatag acttcacgta atcgaaagcg
     1321 gctcctctgg gatgactgcg tttacaggaa cagactggtt ttttgaatcg gacggcaaca
     1381 gcataattaa tatcggttcc ccgaacgggt ttaattctgc tctagcattg ggttcggtga
     1441 gtgacccgaa tggattatta ctacagtata acccggcaac aggtaaagca attcttgcat
     1501 ccacagacgc tggagatgct gtacaaattt ggagcggtaa tagtggattg gcggtggaat
     1561 ttaatactga ccaaactgct agttttgtgc aggagataga tgcagcggct gatacggatg
     1621 ggaaccacat ttttggtcgt atcaggttag gtaaccccgc aggggataac gcgggaattt
     1681 ctcattatga ccatatgtcg cttacaaatt acatgattaa gcaaaatgca aacggattga
     1741 cccgagtgaa ttctccaagt ggtcaggata tggaacttgc gattaacaat acgatgaagc
     1801 ttgaagttga aagtggcgga gatgtgcaca tagttcaagc actggatgtg gacggtaaca
     1861 tcgaattggg cgatacgctt gtgggtaatg tcggaaatgg tgagatacca attgatacag
     1921 caaccaatgg tttagccctg attcgggcaa acacaagcga tggttctgat gatgattaca
     1981 tctcccttgc tggcgggggc gatgtaggta ccgatcgtgg cgggtattta ggcgtctacg
     2041 gcatggagtt cacgacgttc aatttgcacg ggagcattgg cttgtttgcg gcgtcgcatg
     2101 ctaactctaa aatagcaatg aatgtcaatg gaacgaattc aatcataata gacattaatt
     2161 ctcaggtttt tatggcaccg gtgtatgcgg atgcgattgg cacccctcgt cgcgatttat
     2221 ttataaaaga tgatggccaa ttggggtatg tgtcatctac ccgagcggat aaagagcaga
     2281 ttgaagacat aacggatgcg catcttacca aaatatggca gttagaacct aaacgctatg
     2341 agcgcgtaga cgaccccact aacacaaaag aatttggact tattgcggag gacgtggcgg
     2401 cattgggcat cccagggctg gtgtcttatg ctagaaaagc taccatggtt gatgagcgtc
     2461 ctggtaatcc gttggggcca ctttatgaat attcagagga tttaactaaa cctgaaacgg
     2521 ttaactattc tttccttact accatcctcc ttcgagaggt tcaaaaacta cggcaacggg
     2581 tgaccacatt agagggtcac cacacaagat aaggagtaac accatgaaaa agtttacatc
     2641 cattcagatt ttgcttgtag cggccatgtc aggcggaata ctgacggcaa tcttttctga
     2701 gtatccacaa gcggctgcac aaaacgaact cgggaacgta aaggacgctg tagctcgtga
     2761 gatacgaatg aatcctcagg gggcgttaga ctccttcata aacaggattt ttgccaacac
     2821 cgataggacg attacaacaa aaatccttga agaggtaacc ctggatttgg ggattgatat
     2881 cacttcaaag gatgaccctc atgcggattc ctttatccga atatctaggg ctttggaaga
     2941 agctggcata accaatgtcg gagagtgcat tgcggcaatt aaggcggttc cccctgcgtg
     3001 ggaaatccct caggattaga tgcgtctact ttaggagagg tgttatggaa ttcagtgaca
     3061 tggcggaaat gggaacagtc ggggtagtat tatttatcct gtacaaagca attaatctga
     3121 ttccaaaacc t
//
LOCUS       DBNHOH010000477        10184 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1501839, whole genome shotgun sequence.
ACCESSION   DBNHOH010000477 DBNHOH010000000
VERSION     DBNHOH010000477.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10184)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10184)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10184
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1501839"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="AC16V7_08325"
     CDS             complement(<1..821)
                     /locus_tag="AC16V7_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012336.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR6257166.1"
                     /translation="MTDVVIAGIGQTEVSEHWGVSLRELALRAIEAAHQDAGGLQPQA
                     LFVGNMLAPTLSRQAHLGSLVADFAGLTGIEAMTIEAAGASGGAALRTGYLAIASGQV
                     DVAFVVGVEKFSDQVGAPVEEALASSTDSDFESIQGMTPTAQAALLMQRYLHEFGAPR
                     QAFAGFALTAHANGASNPYAMFRKAISAEIYNRAGFVSMPLNLFDVAPNADGAAALVL
                     TRPEMLPPGFPHPLVKIAGSSLVTDTLALHDRPDPLTFQAARVSVERACRQAKITP"
     gene            complement(829..1905)
                     /locus_tag="AC16V7_08330"
     CDS             complement(829..1905)
                     /locus_tag="AC16V7_08330"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HIR6257167.1"
                     /translation="MDHTSLDHLLLKPNRAVGIVGYGAYVPRFRLPASEVSRVWTDGQ
                     AGLPIKEKAVAGIDEDVATMSIEAARNALKRARIEPTHIRAVWVGSESHPYAVKPTST
                     IVAEAIGAVPNVQAADWQFACKAGTEAMVAALGFIGSGMADYAMAIGMDIAQGRPGDA
                     LEYTAGAGGAAYLLGRAEDSLAEIESAYSFVTDTPDFWRRANQKYPEHGQRFTGEPAY
                     FKHITEAAQTLMDATHTTPADYQYAVFHQPNTKFPERVAAQLGFKKEQIATGLLVPVI
                     GNTYAGAALIGLTAVLDIAKPGDRILLVSFGSGAGSDAFALRVTEAIEERRDLAPHTT
                     EYIARRTQIDYATYTRLRGKLVMK"
     gene            complement(1909..3198)
                     /locus_tag="AC16V7_08335"
     CDS             complement(1909..3198)
                     /locus_tag="AC16V7_08335"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HIR6257168.1"
                     /translation="MSEKMSSSRLPGFYNLGIHERRAMLSKFVSAQDLAAMSGEAGLT
                     VEQADHMIENVIGLHGIPLGIALNFVVNGRDVLVPMAIEEPSVVAGASFMARLARAGG
                     GFIAHTTRPEMIGQMQVLEVAEPRSARMALIEQKENLLAEAAEIDPVLKDLGGGPRDI
                     EVRLIEDSPIGAFLVIHLIYDVRDAMGANAVNTACERLAPAVEAITGGRVHLRILSNL
                     ADRRLGRAQCTIPVEQLAFGEYTAEEVRDGVIAAWAFAAADPYRAATHNKGIMNGVDA
                     VVIATGNDWRAIEAGAHAYAARGGRYTSLSTWGVGDQGNLVGTLEMPMAVGVVGGATK
                     VHPTARAALALMGVTSASELAEIIVSVGLAQNLAALRALATEGIQRGHMSLHARQVAI
                     AAGAQGEMIERLAQRLVAEKTVRVDRAEAILRQWQEE"
     gene            3375..5048
                     /locus_tag="AC16V7_08340"
     CDS             3375..5048
                     /locus_tag="AC16V7_08340"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HIR6257169.1"
                     /translation="MALLTREQLEERLAALHRASLELVRELSLDTVLERIVHLAREQA
                     DASYAALGVLDEEGNLVRFIPVGMSAEAVRQMENPPVGMGLIGAMSREYRTIRVANIQ
                     DDPRSEGFPAHHPRMKSFLGVPVLRGDKLLGQIYLTDKESFPEFTEADERVIETLASY
                     AAIAISNARLYEDLLDRDVALDQRNQDLSLINDIATALAGSLDTDEIINQTLIRVMQY
                     LDVDAGEIFLREESEKEMRLALHRGEFAEAFTTRDHFRIGEGFIGLVAQSGQPLITCD
                     LTNDLRFLRPAVVTAGFKCVACIPLTAHGTVVGVLSVASREDRPFDDREVRILTAIGA
                     WAGVAVENARLHRQARRLAVLEERERIGMDLHDGIIQSIYAVGLALEYARVAIDEDPR
                     QSQYKIDQAIEGLNKTIRDIRAYILDLRPRHFVGLNLKEGLQRLVDEYHTNTLSDAVL
                     TAQQDGLPNLPSSHSTALFHICQEALANAAKHAQARRLEVHMWATKDRMLLEVSDNGQ
                     GFDLRKMSVTLGHGLSNMHVRARKVGGDVEITSAPGEGTTILAWVPRRE"
     gene            5048..5911
                     /locus_tag="AC16V7_08345"
     CDS             5048..5911
                     /locus_tag="AC16V7_08345"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR6257170.1"
                     /translation="MDLISSRTNPKIKQIRALRGRQGRQQSDLFLVEGIFHVGEAVAA
                     TQAGKLGLDSVFYAPELLKSEFALELVQGLRRQEGAASIPCYELTRQVFESIAEKDNP
                     QGILAIARPVHYELKALNTSNFSWGVALVAPQDPGNLGTILRTIDAVGASGLLLLDSN
                     LDPYHPSAVRASMGALFWYPLVQASFAEFASWVTERGYTVYGTSAHASTDYRWLTVAG
                     SSDQPSRADQPQAQTYSKPMILLLGSERQGLTEEQVAVCHHLLRLPMLGHATSLNLAV
                     AAGVMLYAMLE"
     gene            complement(6020..7597)
                     /locus_tag="AC16V7_08350"
     CDS             complement(6020..7597)
                     /locus_tag="AC16V7_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6257171.1"
                     /translation="MKFRHISALIALFVVACSQGVISPTSTPTVNHPTLAPAFLPEFA
                     PPGTFQPAACRFILPEDTQEGEDVECGYLFVPERRDAQAQPGGRVIRLAVAIFHPPGG
                     PTRPDPLMYLSGGPGVSALELIRYQFDLLSEPAFSTGRDLILFDQRGVGLSRPALDCP
                     GFDQLALELVDREVDGRQVSDEEIETHLLNSLISCRDELEKIADLSEYNTAASAADVN
                     DLQAALGYPEINLWGGSYGTRLALEVIRRYPQGLRSVVLDAVYPPDVDLYAQAPDNFS
                     RALERMFESCANNPVCSAAFPDVRQVLFETVAQLNTKPAMSEITNPFTGEKYQSWMDG
                     NTILALTFQLLYDSQLRYLIPQYIYDASQGNFTAFDQVRGALIGLRSRTSRGMMLSVQ
                     CQEELAFSSLQDFQSRLARHPELEDTYHNSLMGEIVYSICQEWNVGAADASSKQPVSS
                     AVPTLLMSGEFDPVTPPAWGQQAAKTLTQAYSYEYPGIGHGASVMAGCPQEMMVAFLA
                     NPSVAPNSACIEAMRKP"
     gene            complement(7603..9975)
                     /locus_tag="AC16V7_08355"
     CDS             complement(7603..9975)
                     /locus_tag="AC16V7_08355"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6257172.1"
                     /translation="MKNTIPRGDGKQRIMENTRLIWAITAKDILAALKNKNTISVIIS
                     ALFVVIAYRVLPTLTNLDSSPNVLIYDGDESALVAFMQNSDVLEVHTFPTEEKMKSAL
                     SNADVPELGLVLPAGLDQALEAGQAPELQGYLMYWVDEADAIELKNRVEAEITRLVGT
                     PVDIQVAGNVVYNSTDSSGLGNWAALATVFVVATIGLSMIPHLILEEKQSHTMEALLV
                     SPANAYHVVAAKALVGLFYCLLIASIALGLNHNLVLHWELAIAAVVLGSLFTISLGIL
                     LGTLIENRGQLTLGASILIAPLFFPIILSLMDDLFPAVLIQVFRGVPTTVQFNLLRAS
                     FAGSFPLGKTLLQLVWLVVCAAPGLLATAWLVHRQDRGVPVLSSLRQRYFPKSAPIKD
                     LLAPISAILSRIRQPQESPKAPWQEIATTQVQIPAAKSGFAGSLRVVWAIVAKDFLEA
                     VQNKIVLSIIIGTSFMVASSAIIPLILRSRDIPSAVVYDQGRSTIVRALTGRDDFRIR
                     IVTKQEEMEELITQSPDVIIGLVIPADFDQRAGSSEAISLEGYTNHWADPEKITQRLA
                     FFEEQLGLATWGSVQIDVDGRVLYPSVGPKGQPYVFLLLLSITILTIGVTIVSVSFVE
                     EKEAHTFQALLVSPAGFGQIVTAKALVGIIFCFIASGVVILANAYMVVHWEIALLGIL
                     LGAILAAGIGLLMGGISDNPSTTGMWAALLIIALLFLTFMQEFSRDNWPHLVRLALSW
                     VPGPALIKLLGAAMAWEIPAGLLWTNAIALLSACVLVYGLVFWRIRQFDR"
     gene            complement(9988..>10184)
                     /locus_tag="AC16V7_08360"
     CDS             complement(9988..>10184)
                     /locus_tag="AC16V7_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6257173.1"
                     /translation="VFLTTHYMEEADQLCNRVAILDQGRIAALDTPERLKVKFRKDNQ
                     ATLEDIFVHLTGRSLQQEMV"
BASE COUNT         2214 a         2833 c         2873 g         2264 t
ORIGIN      
        1 ggtgtgatct tagcctggcg gcaggcgcgc tccacggata ctcgggctgc ttggaaggtc
       61 aatgggtctg gccggtcgtg gagcgccagc gtatccgtca ccaggctcga tccagctatc
      121 tttaccagcg ggtgagggaa gccaggtggt agcatttcag gccgggttaa caccagggcg
      181 gctgcgccat cagcattggg ggctacatcg aataagttga gcggcatact gacaaagcca
      241 gctctgttat aaatctcggc gctgatcgct ttgcggaaca tggcgtatgg attgcttgct
      301 ccattggcat gggcggtcag ggcgaagcca gcgaaggctt ggcggggggc gccgaattca
      361 tgcaggtagc gttgcatcag cagggcagcc tgggctgtgg gcgtcatccc ttgtattgat
      421 tcgaaatcac tatctgttga agaggccagc gcctcctcga cgggcgcgcc cacctggtcg
      481 ctgaatttct ccacacccac cacgaaggcc acatctacct gcccagaagc gattgccagg
      541 tagccagtgc gcaaggctgc gcctcccgaa gcgccagccg cttcgatggt catcgcttcg
      601 atgccggtca gtccggcgaa atcggctacc agactgccca ggtgggcctg ccgggagaga
      661 gtgggcgcca acatgttgcc tacgaacagc gcctgtggct gcaggcctcc ggcatcttgg
      721 tgcgctgctt cgatagctcg caacgccagc tcgcgcaggg aaacacccca gtgctcactg
      781 acttctgttt gtccaatacc tgcaatgacg acgtctgtca tttttcctct atttcatcac
      841 cagctttcct cgtaatcgcg tataagtggc gtaatctatt tgtgtgcggc gggcgatgta
      901 ctcggtagtg tgtggtgcaa gatcgcggcg ctcttcgata gcctcggtca cacgcagggc
      961 aaaagcatcg gagccggctc ctgagccaaa agaaaccagc aggatccggt cgccgggctt
     1021 ggcgatatcc aaaaccgcgg taagaccgat taaagccgcc ccggcgtagg tgttgccgat
     1081 cacaggtacc agaagtccgg ttgcgatttg ttcctttttg aatcccagtt gcgcagccac
     1141 tcgctcggga aacttggtgt taggctggtg gaatacggca tactggtaat cggctggggt
     1201 ggtgtgggtg gcgtccatta gagtctgggc ggcctcggtg atgtgcttga aataggctgg
     1261 ctcaccggtg aagcgctgcc cgtgctcggg atatttttgg tttgcacgcc gccagaaatc
     1321 gggcgtatcg gtgacgaagg agtaggcgct ctcgatttct gccagcgagt cttctgcccg
     1381 tccaagcagg tatgctgcac cacccgctcc ggcggtatat tctagggcgt cgccagggcg
     1441 cccctgggcg atatccatgc caattgccat ggcataatct gccatgcccg atccaatgaa
     1501 gcccagggct gccaccatcg cctcggttcc ggctttgcag gcgaactgcc agtcggcagc
     1561 ctgcacatta ggcaccgctc cgatagcctc tgccacaatg gtcgaggtgg gcttgacggc
     1621 gtaggggtgc gattcggaac ccacccatac ggcgcggatg tgggtgggtt cgatcctggc
     1681 gcgtttcaag gcgttgcgag ctgcttcgat agacatggtt gcgacatctt cgtcaattcc
     1741 cgcaacagct ttttctttga tgggcagccc ggcttgaccg tcggtccaca cgcgagacac
     1801 ttcgctcgcc ggcaagcgaa agcgcggtac gtatgcacca taaccgacaa tccctaccgc
     1861 acggttgggt ttcagtaaga gatgatctaa tgaagtatga tccataaatt attcctcctg
     1921 ccactggcgc aaaatcgcct cagcgcgatc cacgcgcaca gttttctcgg ctaccaggcg
     1981 ctgtgctaat cgctcgatca tttctccctg tgctccagcc gcgatagcta cctggcgggc
     2041 atgcaggctc atatgaccgc gctggatacc ctcggtggcc agggcgcgca aggctgccag
     2101 gttttgagct aaaccgaccg agacgataat ttctgccagc tcagatgcgc tggtcacgcc
     2161 catcaaagcc agcgcggcgc gggcagttgg gtgcactttg gtcgccccac cgaccacgcc
     2221 gactgccata ggcatctcca gcgtgccgac caggttaccc tggtcaccca ccccccatgt
     2281 actcagcgag gtatagcgtc caccgcgggc cgcgtaggcg tgtgccccag cttcgatggc
     2341 tcgccagtcg ttaccagtcg caatgaccac tgcgtccaca ccattcataa tgcccttatt
     2401 gtgggtcgcg gcgcggtagg ggtcggcagc ggcgaaggcc caagcggcga tgacgccatc
     2461 gcgcacttct tcggctgtgt attcgccaaa tgccagctgt tccacaggga tggtgcattg
     2521 ggcgcgccca agacgccggt cggccaggtt ggataggatg cgcaggtgca ccctgccgcc
     2581 ggtgatcgcc tcaaccgctg gagccaggcg ctcgcaagct gtgttcactg cattggcgcc
     2641 catggcatcg cgcacatcat atatcaggtg gatgaccagg aatgccccaa ttggcgaatc
     2701 ctcaatcagg cgcacctcta tatcgcgcgg accaccgccc aggtccttta gcactggatc
     2761 aatttccgct gcttcggcca ggaggttttc tttctgctct atcaaggcca ttcgtgcgga
     2821 acggggttca gctacctcca gaacttgcat ctgaccgatc atctccggtc gggtggtgtg
     2881 ggcgatgaat cccccgccgg cgcgggcaag cctagccata aaagatgccc cggctactac
     2941 cgatggctct tcgattgcca tcggcaccag cacatcacgc ccattgacca cgaaattgag
     3001 tgcaattccc agaggtatgc cgtgcaagcc aatcacattc tcaatcatgt ggtcagcctg
     3061 ctctacggtc aagccggcct cgccgctcat tgccgccagg tcttgcgctg agacaaattt
     3121 cgacagcata gcccggcgct catgaatgcc aaggttatag aatccaggaa gacgtgaact
     3181 gctcattttt tcgctcatgg taggtttcgg tctcaagcct tgccatttat acgggtgacc
     3241 cgctggcaaa aactatcagg ttgcctaata accctgcaag ggtcagtaga ttacagctgg
     3301 aattgtgaac ttcagcactt tccctcaaaa gcggaggaaa tggagtaaaa tacatatgtg
     3361 gtgaggtacc ccaaatggcg ttgttaactc gcgagcaact agaggagcga ttagctgctc
     3421 ttcatcgtgc cagccttgaa ctggtgcgcg aactttctct tgacacggtt ctggagcgca
     3481 ttgttcatct ggccagagag caggccgatg cttcttacgc tgctctgggt gtgctggatg
     3541 aagagggtaa tcttgtccgc ttcatccctg taggcatgtc tgccgaggcg gttcgccaga
     3601 tggagaaccc tccggtgggt atgggtttaa tcggcgccat gagtcgggag tatcgcacca
     3661 tccgcgttgc caatatccag gatgaccctc gtagcgaggg tttcccagca catcatccgc
     3721 gtatgaagtc ctttctgggt gtaccagttc taagagggga taaactgcta gggcagatct
     3781 atctaacaga taaagaatct ttcccggagt ttaccgaagc tgacgaacga gtaattgaga
     3841 cgttggcttc ttatgcagct attgctatca gcaacgcgcg tttatatgag gatttgctcg
     3901 accgcgatgt ggcattggat cagcgtaacc aagatctatc gctgatcaat gatatcgcca
     3961 ccgcgttggc tggctcgttg gatacggatg agattatcaa ccaaaccttg attcgggtga
     4021 tgcaatattt ggatgtcgat gctggggaaa tatttctgcg cgaagagagt gagaaagaga
     4081 tgcgcctggc attgcaccgg ggcgagtttg ctgaggcgtt tacgacccgc gaccacttcc
     4141 gtatcggtga aggcttcatt gggttggttg cccaatccgg acagccactg atcacctgcg
     4201 atttgacaaa cgatttgcgc ttcctgcgtc cggcggttgt gacagcgggg ttcaaatgtg
     4261 tggcttgcat accgctcaca gcacatggta cggtggtcgg cgtgctgagc gtggctagcc
     4321 gcgaggatag accttttgac gatcgagaag tgagaatact gaccgccatc ggcgcctggg
     4381 caggggttgc ggttgagaat gcccgtttgc atcgtcaggc acgccgctta gctgtcctgg
     4441 aagagcgtga gcgcatcggc atggatcttc acgatggcat tatacagtcc atctacgccg
     4501 ttggcctggc tctggagtac gcccgcgtgg caatcgatga agaccccaga caatcgcagt
     4561 ataagatcga tcaggctatt gaggggttga acaaaacgat ccgggatatc cgtgcctaca
     4621 tccttgatct gcgcccgcgc cattttgttg gcctgaatct taaagagggt ttgcaacgcc
     4681 tggtcgacga ataccacacc aacacactct ccgatgctgt cctcactgca caacaagacg
     4741 gcttgcccaa cctgccctct tcgcactcca cagccctttt ccatatctgc caggaggctc
     4801 tggccaatgc cgctaagcat gctcaagcgc gacgcctgga ggtacacatg tgggcgacca
     4861 aagaccgcat gctcctggag gtctcggata atggccaggg gttcgatttg cgcaagatga
     4921 gcgtcacgct gggtcacggg ctttctaaca tgcatgtccg cgctcgcaaa gtggggggag
     4981 acgtagagat cacctcagct ccgggagagg gtaccaccat cttagcctgg gtcccccgcc
     5041 gggaataatg gacctgattt ccagccgcac caaccccaaa attaaacaaa ttcgcgccct
     5101 gagagggcgc cagggacgcc agcagagcga cttgttttta gttgagggca ttttccatgt
     5161 cggtgaagcg gtcgccgcga cacaggctgg aaagctgggg cttgattcag tcttctacgc
     5221 cccagagctg ctcaaaagtg aatttgccct ggagcttgtg caggggctgc gccgccaaga
     5281 gggggcggct agtattccgt gttacgagtt gacacgccag gtgtttgaat ccattgcaga
     5341 aaaggacaac ccccagggca ttctagcgat tgctcgccca gtacattacg agctaaaggc
     5401 gctgaatact tcaaattttt cctggggggt ggcgctggtt gccccgcaag acccaggcaa
     5461 cctggggaca atattgcgca cgatcgatgc ggtgggtgcc agcgggctgc tcctgctgga
     5521 tagcaacttg gatccctacc atcccagcgc ggtgcgcgcc agcatggggg cgttgttctg
     5581 gtacccgctg gtccaggcat ctttcgcaga gtttgcgtct tgggtcacgg agcgaggcta
     5641 cacagtgtac ggcacctcag cccatgcctc gacagactac cgttggctta ccgtggctgg
     5701 ttcgtccgat caaccctcga gagcagacca gccacaggcg cagacttatt caaagcccat
     5761 gatcctgctg cttggcagcg aacgccaggg actgaccgag gagcaggtgg cggtctgcca
     5821 tcacctcttg cgcttgccga tgctcggtca cgctacatcc ctgaatctgg ctgtggcagc
     5881 cggggtgatg ctgtatgcaa tgctcgagta gctctacact atctcgaaat tgatctacta
     5941 accccctttt accttcccaa ctgcgtaact tcagaacaag cgattggtga cctgcccgaa
     6001 attaaagcag gtgtttaaat tatggcttgc gcattgcttc gatgcaggcg ctattggggg
     6061 cgaccgaagg attggcgagg aaggcaacca tcatctcttg cgggcagcca gccataacgc
     6121 tggcgccgtg cccgattccc ggatattcat agctatacgc ctgtgtaagc gtcttagctg
     6181 cctgctgtcc ccacgcaggc ggggtgacag gatcaaattc accgctcatc aatagggtgg
     6241 gaacagcgct agaaactggt tgtttgctgc tggcgtctgc cgccccgaca ttccattcct
     6301 ggcagatgct atagacgatt tcacccatta gcgaattgtg atacgtatct tccaactcag
     6361 ggtgtcgggc aagcctgctt tggaagtctt gaagcgagct gaaggcaagc tcctcctggc
     6421 attgtaccga taacatcatc ccccggctgg tgcggctcct caaaccaatc aatgccccgc
     6481 gcacctggtc gaaggcggtg aagtttccct ggctggcatc atagatatac tgcggaatta
     6541 gatagcgcag ttggctgtca tagagcaact ggaatgtcag cgccaggatg gtgttcccat
     6601 ccatccagct ctggtacttc tcgccggtga atgggtttgt aatttcactc atcgcaggtt
     6661 tggtgttcaa ttgggcaact gtctcgaaca gcacttgacg cacatctgga aaagctgcac
     6721 tgcataccgg gttattagcg cagctttcga acatgcgctc cagggcacgg gaaaagttgt
     6781 ctggggcttg cgcgtaaaga tctacatcgg gcgggtaaac cgcatccagc accacgctac
     6841 gcagcccttg tgggtaacgg cgtatcactt ccaatgccag gcgggtgccg taggaacctc
     6901 cccataagtt gatttcaggg taacctaacg ctgcctgcag gtcgttcaca tcggcagcgc
     6961 tggctgccgt gttgtattcc gaaaggtcgg cgattttctc tagttcatcc cggcacgaga
     7021 tgaggctgtt taacaaatgc gtctcaattt cttcatcgct aacctggcga ccgtccacct
     7081 ccctgtcaac cagttccaag gccagttggt caaaaccagg gcagtcaagg gctggacggg
     7141 ataaacctac ccctcgctgg tcgaacaaaa tcaggtctct gcctgttgag aatgctggct
     7201 cagaaagtag atcaaattgg tagcggatca gctcgagggc gctgactccg ggtccgcccg
     7261 agagatacat gagcgggtcg ggccgggttg gccctcccgg tgggtggaaa atcgctacag
     7321 ccaggcggat aacgcgcccg cctggttgtg cttgcgcgtc gcggcgttct ggcacaaaca
     7381 ggtagccgca ttcgacgtct tctccttcct gtgtgtcctc cggcaggatg aagcggcagg
     7441 cggcaggctg gaaggtgccc ggtggagcga attcaggtaa gaaggcgggc gctagcgtgg
     7501 ggtggttgac tgtgggggta gatgtcgggc taattacccc ctggctgcag gcgaccacga
     7561 atagcgcgat aagcgcagag atatgcctga acttcatggc atttaccggt cgaattggcg
     7621 gatgcgccag aacaccagcc cgtataccaa aacacatgca gacagcaagg caattgcatt
     7681 cgtccagagc aatccggcgg ggatctccca tgccatcgct gcccccaata gcttgatcag
     7741 ggcggggcca ggaacccagg agagtgctaa tctgaccaag tggggccagt tgtctctgct
     7801 gaattcctgc atgaaggtca aaaagagcag ggcaatgatc agtaaggcgg cccacatccc
     7861 ggtcgttgac gggttatcag agattccacc cataagtaag ccgatccctg ctgccaggat
     7921 cgcaccaagc agaataccca gcaacgctat ttcccaatgg acaaccatgt aggcgttcgc
     7981 caagatgacc accccggaag caatgaagca gaaaatgatc ccaaccaagg ctttcgcagt
     8041 tactatctgt ccaaaaccgg ctggagacac cagcagagcc tggaaggtgt gggcctcttt
     8101 ctcctcgaca aacgagacgg agacgatggt gacaccaatc gtcaggatcg tgatacttag
     8161 aaggaggagg aatacgtacg gttggccttt tggcccaacc gatggataca ggactcgccc
     8221 gtccacatcg atctgcacac tcccccaggt tgccaggcca agttgctcct cgaaaaaggc
     8281 cagtctttga gttatctttt ctggatcagc ccaatggttt gtgtaccctt ccaggctgat
     8341 tgcttcactg ctgccggccc tctgatcaaa atctgctgga atgaccaagc caatgatcac
     8401 atcaggagat tgcgtgatca attcctccat ctcctcttgt tttgtgacaa tccgtattcg
     8461 gaaatcatcc cgaccggtga gcgcccgtac aattgttgag cgaccctggt cataaacaac
     8521 cgccgaaggg atatccctag agcgtagaat aagggggatt atcgcgctgg aggctaccat
     8581 gaagctggtg cctataatga tcgacagtac gattttattt tgcacggctt caagaaagtc
     8641 ttttgccaca atcgcccaga caacgcgcag gctcccagcg aaaccggatt ttgcggcagg
     8701 gatttgcacc tgagttgtgg cgatctcctg ccatggagct ttcgggctct cttgcggttg
     8761 gcgaattcgg gatagaatgg cgctgatcgg tgcaagcagg tcctttatag gggccgattt
     8821 gggaaagtaa cgctggcgca ggctagacaa aacaggaaca ccccgatctt gtcggtgcac
     8881 caaccaggct gtcgccagca gacccggggc ggcacatacc actaaccaga ctaactggag
     8941 caatgttttg cccaaaggga aactgcccgc gaaggaagca cgcaatagat taaattgcac
     9001 agttgtcggt acgccccgaa agacctggat gagaaccgcg gggaacaggt cgtccatcaa
     9061 ggacaagatg atcgggaaga acagcggggc gatcagaatc gaggctccta gggtaagctg
     9121 gccgcgattt tcgatcaacg ttcccagcag aatcccaagc gaaatcgtga acagcgagcc
     9181 gagcaccacc gctgcgatgg caagctccca atgcagcacc aggttgtggt tcaggcccag
     9241 cgcaatgctt gcaatgagca agcagtagaa caatccaacc agcgccttag ctgccaccac
     9301 atgatacgcg ttggctgggg aaaccagcag tgcttccatg gtgtgagatt gcttctcctc
     9361 cagaattagg tgtgggatca tcgataggcc aatcgtggcg acaacaaata ctgttgccag
     9421 ggcagcccag tttcccaaac cgcttgaatc ggtgctgttg taaaccacgt tcccagcaac
     9481 ctggatatct accggagtcc ccaccagccg ggttatctct gcttctacac gattcttcaa
     9541 ttcgatggca tctgcttcat ccacccagta catcaaatag ccctgcaatt ctggcgcttg
     9601 gcctgcttct agagcctggt caaggccggc gggcagcacc aggccaagtt ctggcacatc
     9661 tgcattcgaa agcgcactct tcatcttttc ctcggttgga aaggtatgca cctcaaggac
     9721 atcactgttt tgcatgaaag cgaccagggc tgattcatcg ccatcgtaga tgagcacatt
     9781 cggtgagctg tctagatttg ttaacgtagg caagacgcga tacgcaatca cgacaaaaag
     9841 tgccgatatg atcacagaaa ttgtgttttt gttttttaag gctgcgagaa tatctttggc
     9901 ggtgatggcc cagatcaaac gggtgttttc catgatgcgc tgctttccat cacctctagg
     9961 gatagtattt ttcattgctt ctcctgccta aaccatttct tgctgcagag acctgccggt
    10021 caggtgcacg aatatatctt ccagggtagc ctggttatct tttcggaatt tgacctttag
    10081 ccgttcaggt gtatccagag cggcgatgcg accctggtct aagatcgcaa cccgattgca
    10141 tagctggtca gcctcttcca tataatgggt ggtcagaaag accg
//
LOCUS       DBNHOH010000478         2732 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1245770, whole genome shotgun sequence.
ACCESSION   DBNHOH010000478 DBNHOH010000000
VERSION     DBNHOH010000478.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1245770"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1390..2010
                     /locus_tag="AC16V7_08365"
     CDS             1390..2010
                     /locus_tag="AC16V7_08365"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003955703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6257174.1"
                     /translation="MDQQPSNIQPFDPQPVTLIGHTVRLEPLSEAHIPDLAIAGKDEE
                     IWHYMLYGPVINEKSMQLWVKDMLQRQARGTDLPFAVIHLGSGRAIGATRYMEIRPEH
                     RGLEIGGTWYALAHQRTAVNTESKYLLLQHAFETLSCIRVQFKTDLRNERSQRALERI
                     GAVWEGVLRNHMILGDGVYRHSVYYSILIEEWPSVKGKLEVLMTQK"
     gene            complement(2056..>2732)
                     /locus_tag="AC16V7_08370"
     CDS             complement(2056..>2732)
                     /locus_tag="AC16V7_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIR6257175.1"
                     /translation="EDRQEKEYEIPSTARLIVRDGDLVEPGQPLTEGSLNPHQILRIQ
                     GRTACQIYLLSEVQKVYRSQGQNINDKHFEVIIRKMMSKVQVLRPGDSGYLPGDLMDR
                     LEIKRTNEQLLTDSKRVAKSVDVLLGITKASLSTDSFLSASSFQHTIKVLAQAAIAAS
                     EDPLYGLKENVIIGKLIPAGTGFVRGRFGDENLPQYVDSGEDVGDDETDDIFDAEFDA
                     QTFAAD"
BASE COUNT          732 a          691 c          592 g          717 t
ORIGIN      
        1 cgaccagacc aaaaaacagc acccccaagg taattatttc tctggcatcc attgaaggcc
       61 atttaatatg cacaagccta agcgagcttc agcatagcgc gcttccgagt caagaaccgg
      121 gcaggcgtca tcccttaaaa cttgaaatct gaacaatcca tacaataata tcttccacct
      181 ttcaccacat atgcatcttc gcttagagag ataaccttcc tacattttgt gcaccgagat
      241 ggtttaaatt ttggtggatt ctccaatttc tcaaagttgt attcgttcgt gccatagtag
      301 acatacattt cagtttcaat gtcttctcga cactgagaac atgttttcca atctccagta
      361 tgattttcta caaaatgata accacacaac gtgtatcggc gatggttacg gaagcagcta
      421 tttcgcgcat aagaaaacat aacatattga tcctcgtcat cacatatcca ttggttgcaa
      481 catttagttt tggttaagtt ctttttcttg ccgcacaagc cgcatcttgg aattgatcta
      541 acatcttttg tgaggggtct ttttccttgt tttgacattt atctctctcg gaaagaaatt
      601 tattgggtta gattcgggag cacaaacatt catggagaac aatattcaaa cgatgtgcgc
      661 ggaataagta tgtaggcaac tgcttaacag acgtgaatac gctcgatagt ttcctatgtg
      721 cagaagtaag aaatattctt aattctacta tggtatgttg gattggctat gctgaaatcc
      781 cctctaaaac catagcaagc aatccgattt ctacccagat tgctgctccc agagaaaatc
      841 ccagcaatgc ggttgcctca tcttcacacc taggaattcg acctccaacg accagaccca
      901 gccaggctac aaagaagcct tatacgatgg agcatggcag gccgacaact caacagagag
      961 attacctgca tttatcccat aaactttcat aaaatcgtat gactcaaggt agccagcggg
     1021 tatccacgtg ggcttgacgt acagctggac aacatcatga agagtatgga tggaagaatg
     1081 aaaagtgaag taatgctttc cattgatccc atcccttatg tggcctaacg ataactcaat
     1141 tggcagcacc ttcagaacta cctacgccaa tttatttatt cacttttttg cagattagac
     1201 tgacacagct aggaccagga tataatttct gtaaccatta atcacacctc tgacgccaga
     1261 ccctttgagt ttattattgg gtaatcatct caaaaatcaa gcgccaaatc tgcaccggcg
     1321 catcaaaatc caggtttctt tataagtaat cagcgcctga tgaaatgccc ccacatgggt
     1381 gtctaagaca tggaccaaca accctccaat attcaaccct tcgatcctca accagtgacc
     1441 ctcattgggc acaccgtgcg attggaacct ctcagcgaag cccatatccc tgacctggcc
     1501 atagctggga aggatgaaga aatctggcat tatatgctct atggaccggt catcaatgag
     1561 aagagtatgc aattgtgggt gaaagatatg ctgcagcgcc aggcgagggg caccgatctc
     1621 ccctttgcgg tgatacacct ggggagcggg cgtgctatcg gggcaactcg ctatatggag
     1681 atccgcccgg agcatcgcgg cttggagatc ggtggcacgt ggtacgcctt agctcatcag
     1741 cgcaccgcgg tcaacaccga atccaaatat ctattactcc aacacgcctt tgagacgctt
     1801 agctgcatcc gcgtacaatt taagactgat ctgcgcaacg aacgctccca acgcgccctg
     1861 gaacgtattg gcgccgtctg ggaaggcgtg ctgcgcaacc acatgatcct gggcgatggc
     1921 gtttaccgtc actcggtata ttacagtatc ctcattgaag aatggccttc ggtaaagggt
     1981 aaattagagg ttctcatgac gcagaaataa aaagtgggcg gtttctgaac cgcccacttt
     2041 ttatttttat ttttatcaat ccgctgcgaa agtttgcgca tcaaactctg cgtcgaagat
     2101 gtcatccgtt tcgtcgtccc caacatcttc gccgctatct acatattgcg gcaggttctc
     2161 atcaccaaaa cggccgcgaa caaatcccgt cccagcaggg ataagcttgc cgataatgac
     2221 attctctttc aagccatata gcggatcttc actggccgcg atagcagcct gtgccagcac
     2281 tttaattgtg tgctggaagg atgaggctga aaggaaggag tcggtactca gagatgcctt
     2341 ggtgatccca agcagaacat ccaccgattt ggctacacgc ttgctatccg tgagcaattg
     2401 ctcattggta cgcttgatct ctaaacgatc cattaaatct ccaggcaagt agccactgtc
     2461 gccggggcga agcacctgga ccttagacat cattttacga ataatcactt caaagtgctt
     2521 atcgttgatg ttttgaccct gcgacctgta gactttctgt acctcagata gcaggtatat
     2581 ctggcacgcc gtgcgtccct ggatgcgcag aatctgatgc gggtttaggg agccttcagt
     2641 cagcggctgg cctggctcaa ccagatcgcc atcgcgcaca atcaggcggg cagtgctggg
     2701 tatttcgtac tccttctctt gccgatcctc at
//
LOCUS       DBNHOH010000479         1546 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_219827, whole genome shotgun sequence.
ACCESSION   DBNHOH010000479 DBNHOH010000000
VERSION     DBNHOH010000479.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_219827"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1530)
                     /locus_tag="AC16V7_08375"
     CDS             complement(<1..1530)
                     /locus_tag="AC16V7_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6257176.1"
                     /translation="MLKTPPSWLVTLGYFIPSLTTQLPYLPSRAPMQEESPRLLEALI
                     NLTMTINTGIPDQPLHLIMDDLQWCDGPTWDFLLQLSHQAENQPLLIYGLCRLEDLPR
                     ERSRLLRALERSELLLHLPLSRLSIEETAELSGYMLPQEESDPIFINRLYKETEGNPF
                     FIIEMVRSMQEAGHSMPSMDRVQGRQDHNLPLGIQRVIEARLDRLSPKSQEFLAISAA
                     IGTAFSFSWLLEIAQVTAEEGISYIEEWLHRGLVHEVGQTYDFNHEKIRQVAYNSLSH
                     ARRQYVHRRIAEVFANTVPPADPATLAYHYARSDQPLKALPFLTQAGEAALRVRSYQE
                     AYQLGLRAVSLLGRMPGPQQRSERIDLNLQLAQAYAFTNDLQRAQEILTETEHLAASL
                     GEEERLGKVYHRLAQIYWLRGQPEVAGDYARRTLRIAEDTNNLSLQMSSLRMLGRVGI
                     ALSAFDDAISNLLRYVRLENETAPTSDLPIVLGYLGIAYDRVGSWKRALETARRGLEM
                     AE"
BASE COUNT          379 a          386 c          418 g          363 t
ORIGIN      
        1 ctccgccatc tccaggccac gccgagcagt ctccagggca cgtttccaag atccgactcg
       61 atcataagca atccccaggt aacctaatac aatcgggaga tctgaggtgg gagcagtctc
      121 attctccaaa cggacatatc gaagcagatt ggaaatggca tcatcaaagg cagatagggc
      181 gattcctacc cgacctaaca tacgtagact gctcatttgc agggataagt tattggtgtc
      241 ttctgctatc cgtaaagtcc gccgggcata gtctccggca acctcaggct ggccacgcag
      301 ccagtagatt tgtgcaagcc ggtgatacac ctttcctaaa cgctcttcct cgcccagact
      361 ggcagccaga tgttcggtct cggttaggat ttcctgagcc cgctgcaagt catttgtaaa
      421 agcatatgct tgggctagtt gcaggttaag atcaatgcgc tcactccgtt gctgtgggcc
      481 gggcatgcgc ccgagcaggc tgacagcgcg taagcctagc tggtatgctt cttgatatga
      541 acgcactcgc aatgccgctt caccagcctg ggtcaggaaa ggtaaagcct ttaagggctg
      601 gtcactgcgg gcgtaatggt aagccaatgt agctggatca gcagggggaa cggtgtttgc
      661 aaatacttcg gcaattctac ggtgaacata ctgtcgccgt gcatgactca agctgttgta
      721 ggcgacctgg cgaatctttt cgtgattgaa gtcataggtt tggcctacct cgtgaaccag
      781 gcctcgatgc aaccactctt caatgtatga gatgccctcc tcagccgtaa cctgggcaat
      841 ttcaagaagc caagagaagc taaaagcagt cccgatcgcc gcggatatgg ctaaaaattc
      901 ctggcttttc ggtgagaggc ggtcaaggcg cgcctcaatt acccgctgga tacccagagg
      961 caggttgtga tcctggcgtc cttgcacgcg atccatggaa ggcattgaat gacccgcctc
     1021 ttgcatggaa cgcaccatct cgatgataaa aaatggattt ccttcggtct ctttatacag
     1081 gcgattgata aaaattgggt cggattcctc ctggggcaac atgtaaccag aaagttctgc
     1141 tgtctcttct atcgatagcc tggagagtgg caggtggagc aggagctcgc tgcgctccag
     1201 ggcacgcaaa aggcggctcc gctcacgggg aagatcttct agtcgacata aaccataaat
     1261 taacaatggc tgattttctg cctggtggga aagttgtagc aggaaatccc aggtggggcc
     1321 atcacaccac tgtaaatcat ccatgatcaa gtgtaatggc tgatctggaa tgccggtgtt
     1381 tatggtcatc gtcagattta taagcgcttc tagcaagcga ggactttcct cttgcatggg
     1441 agcgcgcgat ggaagataag gcagctgagt ggttaatgag gggataaaat atccgagtgt
     1501 gaccaaccaa cttggcgggg tcttcagcat acttggcggc agaaaa
//
LOCUS       DBNHOH010000480         2254 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_284042, whole genome shotgun sequence.
ACCESSION   DBNHOH010000480 DBNHOH010000000
VERSION     DBNHOH010000480.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_284042"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="AC16V7_08380"
     CDS             complement(<1..794)
                     /locus_tag="AC16V7_08380"
                     /inference="COORDINATES: protein motif:HMM:NF017072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-fold NAD(P)-binding domain-containing
                     protein"
                     /protein_id="HIR6257177.1"
                     /translation="MNIGVPKERRPFEFRVGLTPNAIQMLVKDGHKCYVEHEAGLGSG
                     FSDVQYEQAGARIVYSPHEVFGRSDLLIKVSRPILEEIQWLRPGTALAGLLHLNSARK
                     DKIDALLESEITAIALEQIQLPNGRVPVRHALAQIGGQMAPQVAARLLQNEAGGKGIL
                     LGGIVGVPPAEVVIIGAGVVGTCATQGFLGMGAHVSILDIDINALQRIHDRHIGVVTM
                     MANSVNIARAVSYADVVVGAVLVPGERAPIVVTREMLQNMKPRSVI"
     gene            complement(811..1737)
                     /gene="argF"
                     /locus_tag="AC16V7_08385"
     CDS             complement(811..1737)
                     /gene="argF"
                     /locus_tag="AC16V7_08385"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR6257178.1"
                     /translation="MKDFISVSDYSSQDLQEMLDLAVRLKTEWQKGGNPPLLKGKVLG
                     MIFQKPSLRTRVSFDMAMRHVGGDALYLSPAEIGLGKRESIADVARVMSGYVDAIMAR
                     VFNHEHILELARWSSVPVINGLSDYNHPCQAMADALTIQEKFGRLKGLNVTFIGDGNN
                     VAVSLMHICTKLGANFTLASPEGYDLNPIAVDKAKIFAATSGSQIRLLRDPHEAVTDA
                     HVIYTDTWTSMGQEEETQIRERVFPPFQVNQQLVSEAAPDVIVLHCLPAHRGQEITDE
                     VADGPHSALFPQAHNRLHAQKAILVKLLGAAQ"
     gene            1919..>2254
                     /locus_tag="AC16V7_08390"
     CDS             1919..>2254
                     /locus_tag="AC16V7_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6257179.1"
                     /translation="MKLKILVVDDDELVRHTIEELLWLEGYEVAAVETGENAISKLQS
                     EAFDLVLLDLKLPGIDGLDVIRHINRATRDTKFIVLTGHGSLESAIEALRLGAHDYIL
                     KPVTSHVILK"
BASE COUNT          560 a          547 c          571 g          576 t
ORIGIN      
        1 atgattacag agcgcggctt catattttga agcatctcac gtgtcaccac gatgggagcg
       61 cgttctcccg gcaccagtac tgcccccaca actacgtcgg cataggatac ggcgcgagcg
      121 atattgaccg aattggccat catagtcacc acgccaatat gacggtcatg gatgcgctgg
      181 agagcgttga tatcaatatc caatatgctg acgtgcgcgc ccattcctaa gaagccttgt
      241 gtggcacagg tgcccacgac tccagccccg ataattacca cttcggctgg ggggacaccg
      301 acaatccctc caagaagaat accctttccg ccagcttcat tttgtaatag cctggcagct
      361 acttgagggg ccatctgacc accgatttgc gccagagcgt ggcgaactgg aacgcgacca
      421 ttgggaagct ggatctgttc caaggcgatg gcagtaattt cgctttccag aagtgcgtca
      481 attttatctt tgcgagcaga attcaagtgc aacaatccgg cgagtgcagt gcccggccgc
      541 agccattgga tctcttccaa gatgggtcgg gaaaccttga tgagcaggtc ggaacggccg
      601 aagacttcgt gcggggaata aacgattctg gcgccagctt gttcgtattg cacatcgcta
      661 aaaccagaac ccagtccagc ttcatgctca acataacact tatgcccgtc ctttaccaac
      721 atttgaatgg cgttcggtgt caggccgacg cggaactcga atgggcgacg ctcttttggg
      781 accccaatat tcatgggtta cctcactata ttattgtgct gctccaagca gcttcacaag
      841 gatggctttt tgtgcatgca gccgattgtg tgcctgtggg aagagggccg agtgaggtcc
      901 gtcggcaact tcgtcagtga tttcctggcc gcggtgggca ggcagacagt gcagcacgat
      961 cacatctggc gcagcctcag aaacgagctg ctggttcacc tggaatgggg ggaaaactcg
     1021 ctcgcgaatc tgggtttctt cttcctgccc catgctggtc caggtatcgg tataaatcac
     1081 atgtgcatct gtcacagctt catgaggatc gcgcagcaat ctgatctggc tcccgctcgt
     1141 ggcggcaaat atcttggctt tatccactgc aatggggttt agatcatagc cttcggggct
     1201 tgcaagtgta aaattggcac ccagcttggt gcagatgtgc ataagtgaga cagctacatt
     1261 gttgccgtct cctatgaaag taacattcaa gcctttcagg cgaccaaact tttcttggat
     1321 ggtcaaggcg tccgccatag cctggcatgg atggttgtaa tcgcttagtc cgttgatgac
     1381 gggaacacta gaccagcggg caagctctaa tatgtgctcg tggttaaaaa ctcgcgccat
     1441 gatagcatca acgtacccag acatcacacg ggcaacatct gcgattgatt cgcgctttcc
     1501 aaggccaatt tccgccggag ataggtatag agcatcacct cccacatgtc gcatagccat
     1561 gtcaaacgag acgcgcgtgc gcaagctagg cttttgaaag atcattccaa gcactttgcc
     1621 ttttaataga ggaggattgc cccccttttg ccattctgtt tttaatctca ctgccaggtc
     1681 gagcatctcc tgtagatctt gagaggaata atccgagaca gatatgaaat ctttcattat
     1741 ggttttctcc atcaaattga ggttgccatc gcagtatatc atatcggata agggtagaaa
     1801 tgtgctatag ggcagtgatt gatttgatta aagtcacgaa tgcctacata gactacctga
     1861 tgctataatc attttctatc accgtctttc gtcaaagtga gagtttttgg gaagtgctat
     1921 gaaattaaaa attttagtag tagatgatga tgagctggtg cgtcatacca tagaagaatt
     1981 gctgtggttg gagggttatg aagttgcagc tgttgaaact ggagaaaatg ctattagcaa
     2041 gctccaaagt gaagcattcg atctggtttt gttggacctg aagctaccag ggatagatgg
     2101 attggatgta atccgtcata tcaaccgggc cacccgagat accaaattta tcgtgctcac
     2161 tggacatgga tccttggaat cggctattga agctttgcga ctgggagcac atgattacat
     2221 attaaaacca gtcacatcgc acgtcatact taag
//
LOCUS       DBNHOH010000481         4648 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_412081, whole genome shotgun sequence.
ACCESSION   DBNHOH010000481 DBNHOH010000000
VERSION     DBNHOH010000481.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_412081"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..369)
                     /locus_tag="AC16V7_08395"
     CDS             complement(79..369)
                     /locus_tag="AC16V7_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257180.1"
                     /translation="MKIVKGPSHMDSQPAQNADELKKLMHMIEQTKDVEISCDEVFAL
                     IDQYAEMAARGEDVAHLMPLVKHHLEMCADCSQEYEALMRILESPEHRGGLN"
     gene            complement(449..1087)
                     /locus_tag="AC16V7_08400"
     CDS             complement(449..1087)
                     /locus_tag="AC16V7_08400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6257181.1"
                     /translation="MSRRGNDQWISDLQSSGDEREAALADLHQIILRSLPYAINKWIT
                     ADDPRFQALAEEVAQETLLRVLDHLSTFEGRSQFTTWVHKIAVRVALTELRRKRWENV
                     SLDELVEGSETPPLSHLMADSSPSPDDVVEGLDVTERLQRIILEELTEKQRQAMIAIA
                     IKGMPLEEVAQRMGTNRNALYKLMHDTRLRLKRRLAHEGLTPEDVLTIFEKG"
     gene            1260..2564
                     /locus_tag="AC16V7_08405"
     CDS             1260..2564
                     /locus_tag="AC16V7_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6257182.1"
                     /translation="MYSQHEFIPKSRILKIRIELLASVMIRFCMREGSKMYSDSANKE
                     IVVYSTVWCPDCKRAKKFFGEQRIPYINVDIEQDTEAMAYVEKVNNGKRIIPTIVFPD
                     GSILVEPSNSELASHLGLQTKAQRQFYDVIIIGGGPAGLTAATYLAREGLDTLVIEKA
                     GLGGQVGITQVLENYPGFDEGISGGEFAERLSRQARRFGAEILQANEVLDVFPQGPYV
                     CALTGDKIEYGARALLLATGARYRRLNIPGEDDLIGTNVHFCATCDGAFYKGKKLLVV
                     GGGNSGFEEGLFLTKFASQVDIVEFSPQVKASRILQEKIAEQTNMSVTVNHVVKEFKV
                     KDNRLAAVVVEDRGSGEIKELQYDGVFVFIGLSPNSDLVKSKVDLDGYGFITTDMSLM
                     TSKPGLFAAGDVRAGSTKQAASAAGEGATAAIMIREYLKERG"
     gene            complement(2658..4148)
                     /locus_tag="AC16V7_08410"
     CDS             complement(2658..4148)
                     /locus_tag="AC16V7_08410"
                     /inference="COORDINATES: protein motif:HMM:NF024748.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR6257183.1"
                     /translation="MRNRSIFSPLRWFSAFFLLAALVLSAVQLARYSRVRAYFPPGLK
                     IAGIPVGGMDRQQAAQRLLEAYSTPVELIYNNSPIQITPGVLDFQLKLEDMLAAADLQ
                     RTQQPFWQGFWDYLWGRASTPIEVPLRSSHSEARLRSYLEDEISKRYDQPPVPPSPVV
                     GTVNFQPGSPGTELDVENAIPIIVSAARSLSLRQVVLPLQRSEPPRPAFQNLKVLLKQ
                     TIDLTEFDGLTGIYLLDLQTAQEIHMEYMQGEELSLQPDVAYTASSMIKIPIMISVYR
                     RLGENPDSETLKLLNDMITKSGNETADWLMDRVMEPGRAPLIVSEDMQALGLQNTFMA
                     GYFTLGSPLLVRYETPANQRADINTDPDPYSQTTPTEIGMLLEDIYQCAISGGSALQA
                     VYAGEITQGECQAMIDYLKNNHLPALLTAGIPEATQIAHKHGWVSDLNGVINTIGDAG
                     IIYTPGANYVLVIFLHHPQQLVWEQATTMVADLSRAIYNYYNLPSQ"
BASE COUNT         1101 a         1077 c         1244 g         1226 t
ORIGIN      
        1 cccaaactcc cccatcacct ctcgaactgc gtaacttatg tatctttaag attatgacaa
       61 taatgaatgc gtcgcgagct aattcaaacc cccacgatgt tcaggagatt ctaaaatgcg
      121 catcaaggct tcgtattcct gagaacagtc cgcacacatt tcaaggtggt gcttcaccag
      181 gggcatcaag tgggcgacat cttcaccccg cgccgccatt tcggcatatt gatcaatgag
      241 cgcaaacact tcatcgcatg agatctcgac atcctttgtt tgctcgatca tgtgcataag
      301 cttttttagc tcatctgcat tttgcgcagg ttggctatcc atgtgagaag gccccttgac
      361 gattttcaat cgcctcatca agcgtttgat aaaattttca ccagtcatag ttatcccaat
      421 gaataagacg ccttaaaaac tgcttctctt accctttctc aaagatggtc agcacatcct
      481 ctggcgtcaa gccctcgtga gctaacctgc gcttcaaacg caagcgcgta tcgtgcataa
      541 gtttgtagag cgcattgcgg ttggttccca tgcgttgtgc gacttcttct agcggcatgc
      601 ccttaatggc aatcgcgatc atagcttggc gttgtttttc ggttaattcc tccagaataa
      661 tccgctgaag ccgttcagtg acatctaaac cttcgacaac atcatctgga gaaggggaag
      721 aatctgccat caagtgggaa aggggtgggg tttcactgcc ttcaaccagt tcatccagtg
      781 aaacattttc ccaacgtttt cgacgcaatt ctgttaacgc cacccgtacc gcgattttgt
      841 gcacccaggt cgtaaattgg ctgcgacctt cgaatgtgct caggtgatcc agaacgcgca
      901 gtaatgtttc ttgagccacc tcttcagcca gagcctgaaa gcgcggatcg tcagctgtaa
      961 tccacttgtt gatggcgtat ggtagacttc ttaaaataat ctggtgcaaa tctgccaagg
     1021 cggcttcgcg ctcatcgccg cttgattgca gatcgctaat ccattggtca ttaccgcgtc
     1081 tggacatcga tgatcattat acattactcc ctagtaagaa tctgctatac agggttgtct
     1141 atccaaaagc ataggtgaat gaattatcct atatgcaaaa gggtagtaca ctaagggaga
     1201 tcaagatcgc tgataccatg cgattgtcat tggggctagg tacagtcata tggtaaacaa
     1261 tgtatagcca gcatgagttc attcctaaat ccaggattct gaaaatcagg attgaattgc
     1321 tggcgagtgt catgatccga ttttgtatgc gggaagggag taagatgtat tcagattcag
     1381 cgaataagga aatagttgta tatagcactg tctggtgccc ggattgcaag cgcgccaaga
     1441 aattcttcgg cgagcagcgt atcccctata tcaatgtgga tatcgaacag gatacggaag
     1501 caatggctta tgtggaaaaa gtcaataacg ggaagcggat aattccaacc attgtttttc
     1561 cggatggctc tatcctggtt gagccctcca attctgagtt agcttcacat cttggattac
     1621 agacgaaagc gcagcgccaa ttctacgatg tgatcattat tggcggcggt cctgctggtt
     1681 tgaccgcggc tacttatttg gctcgagaag gactggatac tttggtgatt gaaaaggctg
     1741 gccttggcgg gcaggtcggg atcacgcagg ttttggagaa ttatccgggt ttcgatgaag
     1801 gtatctctgg cggagaattc gcagaacgtt taagccggca ggctcgtcgc tttggggctg
     1861 agattttgca agccaacgaa gttttagacg ttttccctca aggtccgtat gtttgtgctc
     1921 tcactgggga taagattgaa tatggagcaa gggcgttgtt attggctact ggcgccaggt
     1981 accggcgcct gaacattcca ggtgaggatg atttgatcgg tactaacgtg catttctgcg
     2041 ccacctgtga cggggcattc tataaaggta agaaattatt ggtcgttggg ggcggcaaca
     2101 gtggtttcga ggaaggcttg tttctcacga aatttgccag tcaggtggat atcgtggaat
     2161 tctcaccaca ggtgaaagcc agccgaattc tgcaggaaaa aatcgccgaa caaaccaata
     2221 tgtctgtaac agtaaaccat gtggttaaag aattcaaggt aaaggataat cgcctcgcgg
     2281 ctgtggttgt tgaagataga gggtccggag aaataaaaga attgcagtac gatggcgtat
     2341 tcgtatttat tggtctttca cccaatagcg atctggtaaa gtccaaagtt gatttagacg
     2401 gctacggatt tattaccacc gatatgtcac tgatgacgag caagccgggc ttattcgccg
     2461 ctggcgatgt gcgagcaggt tcgaccaagc aggccgcttc tgctgctggg gaaggtgcga
     2521 cggctgccat catgattcgc gagtatctga aagaaagagg ttaggggtag aacggctaaa
     2581 ccctttttcg tgttgggttt cactgtctgc ttcgctactg gattctaggg ttattgaggc
     2641 ggttgagtgt agagaattta ctggcttggc agattgtagt aattgtaaat cgcccgcgat
     2701 aggtcggcta ccattgtggt ggcttgctcc cacaccaatt gttgaggatg gtgaaggaag
     2761 atcaccagta cgtagttggc gccaggggtg tagatgatcc ccgcgtcgcc gatagtattg
     2821 atcacgccgt ttaaatccga aacccaaccg tgcttgtgag cgatttgggt ggcttcaggt
     2881 atgcccgcgg tgagcagggc gggcaaatga ttgtttttca ggtaatcgat cattgcctga
     2941 cattcaccct gggttatctc gccagcataa actgcctgca gcgcgctgcc ccctgagata
     3001 gcacattggt agatgtcctc cagaagcatg ccaatctccg tgggtgtggt ctggctgtag
     3061 gggtcggggt cggtgttgat gtctgcacgt tggtttgcgg gggtctcgta gcgtaccagt
     3121 agcggtgagc cgagggtgaa atagcctgcc ataaaggtgt tttgcaatcc caaggcttgc
     3181 atatcttcac ttactatcaa cggcgctcga cctggctcca tcacgcggtc catcaaccag
     3241 tccgccgtct cgtttccaga cttggtgatc atgtcattga gcagctttaa agtctctgag
     3301 tcagggttct ctcccagacg gcgatacacg gatatcatga tgggtatctt gatcatgctg
     3361 gaggcggtgt atgcaacatc gggctgcaag gataactctt ccccttgcat gtattccatg
     3421 tggatttcct gagcggtttg cagatcgagc aggtaaatcc ctgtcagacc atcaaactct
     3481 gtcaggtcga tggtctgctt gagcagcacc ttgaggtttt ggaatgcggg acgcgggggc
     3541 tcggaacgtt gaagcggcaa tacgacttgg cgtagcgata gcgatcgcgc tgcgctcaca
     3601 atgatgggga tcgcattttc aacgtccaat tctgtcccag gagagccggg ttgaaaattt
     3661 accgtaccta cgaccgggct gggggggact gggggttgat cgtagcgttt ggagatctca
     3721 tcttccaaat aagagcgcag gcgcgcttcg gaatgggacg agcgcagtgg tacttctatt
     3781 ggcgtggatg ctcgccccca taggtaatcc cagaagcctt gccagaaagg ttgctgagtg
     3841 cgctgtaaat cggcggctgc cagcatatcc tccagcttga gctgaaaatc gagcacgccg
     3901 ggggtgatct gtatggggga gttgttgtag atcagttcta ccggagttga atacgcttcc
     3961 aataggcgct gggccgcttg ttgtcgatcc atcccgccca cgggtatgcc ggcaattttt
     4021 aggccgggag ggaagtatgc ccgtacccta ctataacgcg ccagttggac agctgagagc
     4081 accagcgctg cgagcaggaa aaacgccgag aaccagcgca gtggagaaaa aatactgcga
     4141 tttctcatgg cttgctatca taccattaaa ccatggagtc attttactgc acgctcaaat
     4201 agggtaatcg catttgggcg attgcccacc actgaggaga gaaaatgggg tgttttggtg
     4261 ggggcatatc cccaccaaaa cacccccaac actcttttta cgggattgcg catgcccctc
     4321 agggtatatt gcaattgccc tgctcaaatc ttgccacccg cagcggggat tacgaagagc
     4381 ctgcatcttc gctcgaatat atcgctaaca atttatctgg agaccaccag cttcactaat
     4441 cgattttgtt catccagggt taagcgggcg taatggcggt gcacgtgggc tgcgttacca
     4501 accactttgc ttaagatcac aatgcgtctt tgttgggttg gtggtattgc attagagttc
     4561 ataccccgag atgtctgtgc tgatgctggc gaggaacttg atgttgcact ctttccatga
     4621 tgtttcatgt gcaattgccc tgtggggc
//
LOCUS       DBNHOH010000482         2104 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_156020, whole genome shotgun sequence.
ACCESSION   DBNHOH010000482 DBNHOH010000000
VERSION     DBNHOH010000482.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_156020"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /locus_tag="AC16V7_08415"
     CDS             complement(<1..1199)
                     /locus_tag="AC16V7_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257184.1"
                     /translation="MKEKARAINFLFRLAATYFICKVLIEAANEYFEYAWGYGSWLGG
                     FTLNGAIGLIALVLAWAMLFALIMLRLWLYDRTIRIEGVLSSWRNRLGPLCGLIIIIP
                     LLTPLWFMQYSRWGGEFIGLPFRLVVFLVSSYMVCVLLTRSHTKLVTLPNLAISILLV
                     TSGLIIGSTAAGITDYPFSLTWSEGNRMWDYSVLFGRAIYDYPSDRPIFAYLDLGRQS
                     LWGLSFLLPNVSIVAVRAWNAFLLTIPYAIFGWIVFMKGAKLGKLWMLAGLWSLAFMS
                     QGPIYTPLVLGAIFVAIAWFTPHWMAFILIAVAGYYLNIGRYTWIFAPAMWASMIYFG
                     VIKISVSKFDPKRVMPAVGAVLAGLFGGYVLPRWQMIFQAATSILRPSDISGQAAAPG
                     MDIVSVEG"
     gene            complement(1196..>2104)
                     /locus_tag="AC16V7_08420"
     CDS             complement(1196..>2104)
                     /locus_tag="AC16V7_08420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6257185.1"
                     /translation="LYFFLKKEKFTVVETFTHSANILGLPLAWAAKIPVRIASHHGRF
                     EGLTSLQERFHRVIINSGLATRMVAVSSCVAELATEKEHINPNKITVIRNGIDMSVEV
                     NLDEEERISLRQSLGVGTKDFLIITVGRLVNQKGHVYLLEAMAEVIKHFPHTKLVIAG
                     NGPLQATLVAKARTLQIEKSVHFAGNRGDVPYLLSIGDCFVLSSLWEGLPIALLEAMR
                     AGLAIIATKVEGITEVITDGIHGLLVPAADARALAQSIMRIISDDVARERIGRQGRTE
                     VMRTYSYERMCEEYATLFQRELEARG"
BASE COUNT          606 a          539 c          450 g          509 t
ORIGIN      
        1 ccctctacgc tgacaatatc catccccggc gcggcagcct ggccagatat atcgctaggc
       61 cttaagattg aagtcgctgc ctggaaaatc atttgccagc gaggcaggac ataccctcca
      121 aaaagtcccg ccagaacagc tccaacagct ggcatgaccc gcttagggtc aaacttcgat
      181 acggaaatct ttatcacccc aaaataaatc atgctggccc acattgctgg cgcaaatatc
      241 caggtgtacc gaccgatatt gaggtagtac ccagccacag cgattaagat aaaggccatc
      301 caatgcggag taaaccaagc tatcgcaacg aagatagcac ctagtaccaa gggggtgtag
      361 attggaccct gactcataaa agctaggctc cataatccag ccaacatcca aagcttccct
      421 aactttgcgc ctttcataaa aacgatccag ccgaaaattg catagggtat cgtgaggagg
      481 aaagcattcc aagctctaac cgccacgata gagacattcg gtaggagaaa tgagagtccc
      541 caaagcgatt gtcttccaag atctaaatag gcaaaaatgg gtcgatcgct ggggtaatcg
      601 taaattgcgc gtccgaataa tacggaataa tcccacatcc ggttgccttc tgaccaagtc
      661 aaagagaaag gataatcagt tatccccgca gcagtgcttc caattattaa gccgctggtc
      721 accaggagaa tgctaatggc tagattgggc aaggtgacca acttggtgtg gcttcgtgtt
      781 aataatacgc aaaccatata actcgacact aaaaatacca ccaggcggaa tggcaatcca
      841 ataaattcac caccccatcg ggaatattgc ataaaccata atggggttag taagggaata
      901 atgattatta acccacacag cggacctagg cgatttcgcc aggacgataa tactccttca
      961 atgcgtatcg ttcgatcgta cagccatagt ctaagcatga tgagagcaaa cagcatagcc
     1021 catgccagca caagcgcaat taatccaata gccccattta gcgtgaagcc acccagccaa
     1081 ctaccatacc cccaagcata ttcaaaatac tcgtttgctg cctcgatgag aactttacaa
     1141 atgaaataag ttgctgcaag gcgaaataga aaatttatgg ctcgggcttt ttctttcaac
     1201 ctcgcgcctc caactctcgc tggaataaag ttgcatattc ttcacacatt cgttcatagc
     1261 tatatgttct catgacctcg gttctaccct gtctgccgat tctttcccgg gcaacatcgt
     1321 cagatattat ccgcatgatg gattgtgcga gagcccttgc atccgccgca ggtacaagca
     1381 aaccatgtat gccatctgtg atcacctctg tgatgccttc cactttggta gcgatgattg
     1441 caagacccgc ccgcatggct tcgagtaggg cgatcggtaa accctcccag agagaagata
     1501 atacaaaaca atccccaatg cttagcagat atggcacgtc accccggtta ccggcaaaat
     1561 ggaccgattt ttcgatttgc aatgtacgag ctttcgccac caatgttgct tgtagggggc
     1621 cattacctgc aattaccagt ttggtatggg gaaaatgctt tattacctct gccattgcct
     1681 caagtaaata aacatgccct ttctgattta ccaggcgacc aacagtaatg attagaaagt
     1741 ctttggtgcc gactcctagc gattgtcgga gagaaattcg ctcttcctcg tctagattca
     1801 cttccaccga catatcgatg ccattacgta tcactgtgat tttgttgggg ttaatatgtt
     1861 ctttttctgt agccagttca gctacacagc ttgagactgc caccattcgc gtggctaagc
     1921 cgctattaat aatcacccga tggaagcgct cttgcagcga ggtaagccct tcaaaacgac
     1981 cgtgatgcga ggcgatgcgc acgggtattt ttgccgccca tgctaaaggt aaccctaaaa
     2041 tattggcgct atgggtaaaa gtctcaacca cagtaaactt ttcttttttt aggaagaaat
     2101 agag
//
LOCUS       DBNHOH010000483         1848 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_284603, whole genome shotgun sequence.
ACCESSION   DBNHOH010000483 DBNHOH010000000
VERSION     DBNHOH010000483.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_284603"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /gene="proB"
                     /locus_tag="AC16V7_08425"
     CDS             complement(<1..1087)
                     /gene="proB"
                     /locus_tag="AC16V7_08425"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016959686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HIR6257186.1"
                     /translation="MQVEKTMNFQRIVIKLGTSTLTAGTPGISPPRLIELVRQMAELY
                     RPGRELLLVTSGAIAAGREWLKFPDLPKDIPIKQMLAAVGQPRLMALYEQLFRMYGIT
                     VGQVLLTRTDLANRRGYLNARNTLAALLSQGVVPIINENDTVATEEIRVGDNDTLSAL
                     VANLIEADLLVILTDQPGLLTADPRKDPSAQLVTEIREAEIPEALWQAAGGSTSRLGT
                     GGMITKLQAADLARRSGAYVFIARGSDADVLLRLVNGEALGTRFLPLATSVESRKRFL
                     LTGGRSRDRLVVDTGASQALHAGGSLLPVGVTSVEGGFERGDPVRVLDQQGREIARGL
                     VNYSAADLARVCGRHSSEIEKILGYMYG"
     gene            1191..>1848
                     /locus_tag="AC16V7_08430"
     CDS             1191..>1848
                     /locus_tag="AC16V7_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR6257187.1"
                     /translation="MSKLLILGTANGLTMREWSDDGWRVSRQGQSGLIGLRVTSAAAR
                     DGGMLAGTRDGIFRSDDLGLTWRDVSQGLTTRYVRWLAIYPDNNNIVLAGTEPAGIYI
                     SKDGGLSWRTCPEVAMLRDQRGWSLPYSPEAGCVRGFAFHGQRVYAAVEVGGVLVSED
                     SGESWRLADGSQEVTFYPPKGAIHPDVHSIEVHPSSRNIVYAPTGGGFFRSGDGGATW
                     T"
BASE COUNT          371 a          577 c          528 g          372 t
ORIGIN      
        1 caccatacat ataacccagg atcttctcaa tctcgctgga gtgtcgcccg cagacgcgcg
       61 caaggtcagc cgccgagtaa ttgaccaggc cacgggcgat ctcccgccct tgctgatcca
      121 gcacacgtac cgggtcaccg cgctcaaaac caccttccac cgaggtcacg cctacaggta
      181 gcaagctacc tccagcatgt aatgcctgcg acgcgcccgt gtccaccacc aatcgatccc
      241 tcgagcgtcc gccagttaac aggaaacgtt tgcggctctc gaccgaggta gccagcggga
      301 gaaaccgcgt gccgagtgcc tctccattca ccaggcgcag caagacatcc gcatcgctgc
      361 cacgcgcgat gaacacgtat gccccagagc gccgcgccag gtcggcggcc tgcagcttgg
      421 tgatcatgcc acccgttccc agccggctgg tcgagccacc cgctgcctgc cacaaagcct
      481 ccgggatctc agcctcccta atttctgtca ccagctgtgc cgagggatcc ttgcgggggt
      541 cagcggtgag caacccgggc tgatcggtga gtatcaccag gaggtcggcc tcaatcaggt
      601 ttgccaccag cgcggatagg gtatcattat ccccaacgcg aatctcctcc gtggcgacgg
      661 tgtcattctc attgatgatg ggtaccacgc cttgggagag cagggctgcc agcgtgttac
      721 gtgcgtttag ataacctcgc cggttagcca gatcggtgcg tgtcagcaac acctgaccta
      781 ccgtgatgcc gtacatacga aagagctgtt cgtataaagc catcaagcgc ggctgtccaa
      841 ccgccgccag catctgcttg atcggtatat cctttggcaa atcagggaac ttcaaccact
      901 ctctcccggc ggcgatcgct ccggaggtga ccaataacag ctctctgcct ggacggtaca
      961 gctcggccat ctgccgaact agctcgatca ggcgcggtgg agagataccc ggcgtaccgg
     1021 cggtgagcgt tgatgtaccc agctttatca caatgcgctg gaaattcatg gtcttttcaa
     1081 cctgcattta tcccaaccct gtcggatttg tcgtattata ctatgagctg atacgtattg
     1141 gacgcttggg accccttcaa ccaagctacg cctctgtagg agaaaacatt atgagtaaac
     1201 ttcttatcct tggaactgcg aacgggctga ccatgcgcga atggagcgac gatggctggc
     1261 gcgtgtctcg gcaaggccaa agcgggctga taggtctgcg ggtcaccagc gctgccgccc
     1321 gagatggagg gatgcttgca gggacacgcg atggcatttt caggtcagac gatctcggtc
     1381 ttacctggcg agacgtcagc cagggactga ccacgcgcta tgttcgctgg ctggcaatct
     1441 atccggacaa taacaacatt gtgctcgctg gcaccgagcc ggctggcatc tatatttcaa
     1501 aggatggcgg gctaagctgg cgcacctgcc ccgaagtagc catgcttcgc gaccagcgcg
     1561 gctggtcact gccttattcg cctgaggcag gctgcgtgcg cggtttcgcc ttccacgggc
     1621 agcgcgtcta cgccgcggtc gaagtgggcg gcgtgctcgt ctcggaagac agcggcgaaa
     1681 gctggcgcct ggccgatggc agccaggagg tgactttcta tcccccaaag ggagcgatac
     1741 atcccgatgt gcactccatc gaggtgcacc cctcttcacg taatattgtg tatgccccca
     1801 ccggtggcgg gtttttccgc tctggggatg gcggcgcgac ctggaccc
//
LOCUS       DBNHOH010000484         2454 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1630502, whole genome shotgun sequence.
ACCESSION   DBNHOH010000484 DBNHOH010000000
VERSION     DBNHOH010000484.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1630502"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="AC16V7_08435"
     CDS             complement(<1..687)
                     /locus_tag="AC16V7_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257188.1"
                     /translation="MPTKNRYWFVIVLVISALLLVFTWPVLSSLDESPHVSSASAQQG
                     CTTLVDPKEIPAPALIDFDDLPNAKVIDTSYQTSHGVTFGETDTTEVIISNKISGPQS
                     MPNVAINNDLTRNGGPIVIRFDKPRTHVGFWIGNGERINANLFAYDANGSIICELFEI
                     AVPGPLSKFIGLADPEGRIAKIDLDYLDPAFSETIDDLYFSPKPGASPTRTPLPTWTP
                     VPSPTPTLGPD"
     gene            complement(986..1477)
                     /locus_tag="AC16V7_08440"
     CDS             complement(986..1477)
                     /locus_tag="AC16V7_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257189.1"
                     /translation="MKQSTMIWFALFALVLAACSPAQAPVPSDIQPTNQPSSVLPSTL
                     QPAVKDLTRSDSQGAVVVEIKPLNLDNPADTLLFEVSLNTHSVDLSMDLAALATLTTD
                     TGHTVEAAHWDAPRGGHHVSGTLSFPATWDGAPLLEGASMLTLSIQNLDASLRMFNWN
                     LSH"
     gene            complement(1477..1971)
                     /locus_tag="AC16V7_08445"
     CDS             complement(1477..1971)
                     /locus_tag="AC16V7_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257190.1"
                     /translation="MTLSRRIIWSVGAGLAGMLFLSSLFFGVVSWAGGLEHAASLFWQ
                     DRRFIVPIILGFGIQTGLYTILKKQLFLPTAKVGPSGVLTGTGGATSTAAMVACCAHH
                     VTDVLPILGLTAAATFLAQYRTMFMLVGLGTTLVGVAVMLFILYTERRKVLKMSAKAT
                     LPEA"
     gene            2204..>2454
                     /locus_tag="AC16V7_08450"
     CDS             2204..>2454
                     /locus_tag="AC16V7_08450"
                     /inference="COORDINATES: protein motif:HMM:NF025170.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCYCGC motif-containing (lipo)protein"
                     /protein_id="HIR6257191.1"
                     /translation="MKRTVYKTLLPLMVILMIFSGSLSACSSDGVAENEHDLAMRPLD
                     ELPAEVKSAPVVVQQAYQFAAANPEIMTHIPCYCGCGEMG"
BASE COUNT          572 a          586 c          723 g          573 t
ORIGIN      
        1 gtctgggcct agggtgggcg tgggtgaggg caccggtgtc catgtgggga gcggggtgcg
       61 cgtggggctg gcgccaggct tgggggagaa atacaggtcg tcgatggttt cgctaaaggc
      121 aggatccagg taatctagat cgattttggc aatgcgccct tccgggtctg ctaaaccgat
      181 aaatttggac aggggtccag gcacggcgat ctcaaacagc tcgcagatga tggaaccatt
      241 tgcatcgtag gcgaagaggt tggcattgat cctctcgcca ttgccgatcc agaacccgac
      301 gtgcgtcctg ggcttgtcga agcggattac aattggccct ccgttgcgcg tgaggtcatt
      361 gtttatggcg acgttcggca ttgattgcgg tccgcttatc ttgttggaga tgattacttc
      421 ggtagtatcg gtttctccaa aggtgacacc gtgggacgtt tggtagctgg tgtcgatcac
      481 ttttgcgttg ggcaggtcat cgaagtcgat gagcgcgggg gcggggatct cctttggatc
      541 gaccagcgtg gtgcagccct gctgggcgct ggctgaagaa acgtggggac tctcatccag
      601 cgatgacaag acaggccaag tgaataccaa cagcaaggct gaaatcacca ggacaataac
      661 aaaccagtaa cgattcttgg tcggcatggc gatacctcac aaacatttcg gattttgccg
      721 cgttgttcta tctggaaatg caacccccca tgggagtgaa cgatgtggag caattatacc
      781 ttattgccct aaaaagtatg atataagact tgtgtttcct ctagttctgc cttgatgatg
      841 cgctggcagt tggattacca atctgtcttg gatatagagc ggggttaatt ggaatattga
      901 aaatctgact atatatggct ttgcaccaag gaattatccg atactcacat taaataaaaa
      961 attgggcgag gagtaaaaaa gcaccttaat gcgataaatt ccagttgaac atccgtaagg
     1021 aagcatccag gttttggatg ctcagggtga gcatgctggc accttcgagc aatggagccc
     1081 catcccaggt tgccggaaaa gagagcgtgc ctgagacgtg atgtccaccg cgtggcgcat
     1141 cccagtgcgc agcttcaacc gtatgccccg tatctgtggt caatgtggcc agggcggcta
     1201 ggtccatgct caggtcgact gagtgggtgt tcaggctcac ttcaaaaagg agggtgtctg
     1261 caggattatc caggttaagc ggcttgatct caaccaccac cgcgccctgg ctgtctgagc
     1321 gagttagatc cttgaccgcg ggttgcaatg tgctgggcaa cacactggac ggctgattgg
     1381 ttggctgaat atccgaggga acaggtgcct gtgctgggct gcaagctgcc agcacaagcg
     1441 cgaataaggc aaaccaaatc attgtggatt gtttcattat gcctccggca gggtggcttt
     1501 cgccgacatt ttgaggacct tacggcgttc tgtgtaaagg atgaacaaca tcacagctac
     1561 accgaccagg gtggttccca atccgaccag catgaacatt gtgcgatact gggcaagaaa
     1621 agtcgctgcc gccgtaagcc caaggattgg gagtacatcg gtgacgtggt gggcgcagca
     1681 ggccaccatg gcggctgtgg atgtggctcc gcctgtcccc gtgagcactc ccgaaggtcc
     1741 caccttggca gtgggcagga aaagctgctt tttaaggatg gtatagagtc ctgtctggat
     1801 tccaaatccc agaatgatcg ggacaatgaa tctccgatct tgccagaaca ggctggcggc
     1861 gtgttccaat cctcctgccc aggagacaac gccaaagaac aggctgctca gaaaaagcat
     1921 cccggctagg ccggctccta ccgaccagat aatgcgtctc gataatgtca tggcgaagag
     1981 ctcctgtaat gttcgaatac aacgcgtccg agttaatcgt atcggtttgg gcgtttttgg
     2041 tcatgcgcta ctcagctatt cttggagtag gcaatagtgc ctatacgcgc ccccgtcaga
     2101 ttgcgctgac tgtcacgaag caggttcctt ataatgggtg cagattgaag aaaacctatg
     2161 gcagattttg cgcctgcaat aggatgagag gagatcgcgc ttcgtgaaac gcaccgtgta
     2221 taaaaccctt ctacccctga tggtaatatt gatgattttt tccggttcgc taagcgcttg
     2281 cagcagcgat ggcgttgcgg agaacgagca tgacctggcg atgagaccgc tggatgagct
     2341 gccggcggaa gtcaaaagcg caccggtagt ggtgcagcag gcatatcaat ttgctgcagc
     2401 gaatcctgag atcatgaccc acatcccctg ctactgcgga tgtggcgaga tggg
//
LOCUS       DBNHOH010000485         1443 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_156285, whole genome shotgun sequence.
ACCESSION   DBNHOH010000485 DBNHOH010000000
VERSION     DBNHOH010000485.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_156285"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="AC16V7_08455"
     CDS             complement(<1..541)
                     /locus_tag="AC16V7_08455"
                     /inference="COORDINATES: protein motif:HMM:NF013126.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HIR6257192.1"
                     /translation="MERAESLLDDLSPEERVGQLFLATFQGSDIDGNSEINDLITDYH
                     IGGVILLARNDNFTADPDELERISSMNRRLQLIRWSAAQQTRITASGESFTPTFVPLF
                     ITIAQEGDGYPYDQILNGLTPLPNEMAMGATWNPDLVSQVGNVLGQELSALGFNLLLG
                     PSLDVLETPNLEGGSSLGTR"
     gene            complement(701..>1443)
                     /locus_tag="AC16V7_08460"
     CDS             complement(701..>1443)
                     /locus_tag="AC16V7_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257193.1"
                     /translation="YLLARATHAPNLYFASAIGQSICRYPAPLGLTRIESLWLDRSLM
                     NTGFARVATEILPNLRPKEFFRPGQIDPAGNFNNIAIGKTYLTDVKKRPRLRLPGSGG
                     IPDVTTFLDDIYLYVPRHSRVTFVAHLDVRSGLGHAPERKLGSGARYLISDLGEFDFD
                     GGRMRLTSYHPGTSIERIASRTGFELDIAPDVHETPAPSPEELHILREEIDPLGIRKL
                     ELLSGKARHELLHQIINAESAIQVIQAR"
BASE COUNT          365 a          340 c          387 g          351 t
ORIGIN      
        1 tgcgcgttcc taagctgcta cccccttcga gattcggtgt ttctagaaca tccagagatg
       61 gtcctaatag caaattaaac cctaacgcag acagctcttg tcctaataca tttcctacct
      121 gagagactaa atcaggattc caggtggctc ccatggccat ttcgttgggc aggggagtga
      181 gaccattcaa aatctggtcg tagggatagc catcgccttc ttgggcgatt gttataaata
      241 aggggacgaa tgtgggcgtg aaactctccc cggacgcggt gatacgcgtt tgttgagctg
      301 cagaccagcg gatcaactgt agtcggcgat tcatggatga gatgcgttcc agctcatctg
      361 gatctgctgt aaagttgtca ttgcgcgcaa ggaggatgac gccgccaata tgatagtcgg
      421 tgatcagatc attaatttcc gagttaccat cgatgtctga tccctgaaaa gtcgccagga
      481 agagctgacc gacgcgttcc tctggagaga gatcatctag caaggattca gctcgctcca
      541 ttggggttgc cacctgataa gctggcgaag cttggacact agccaaagga ttaatgaatg
      601 ccaggagtat cgatattaat acaataaatg taatccaccc ggaagtcgat gaactcccat
      661 tagattgact ccgagtaaga tgtgaaagaa acctcatgat ttatcgagcc tgaatgactt
      721 ggatggctga ttctgcattg attatctggt gcaataattc atgcctagct ttaccgctta
      781 ataactccag cttgcggatg cccaacgggt cgatctcctc tcgtaggata tggagttctt
      841 caggtgatgg agctggtgtc tcgtgcacat caggggcaat atcaagttcg aagccagtac
      901 gggacgctat gcgctcaatg ctggttccag ggtggtagct ggtcaagcgc atacgacccc
      961 catcgaagtc gaactcgccc aaatcggaga tcaggtatcg ggcccctgag ccgagttttc
     1021 tttcgggagc gtgacccagt ccagaacgca catccaggtg tgcaacaaac gtcacgcgcg
     1081 agtggcgtgg cacgtaaaga tatatatcgt caagaaaagt ggtcacatct gggatgcctc
     1141 cagagccggg taatcgcaga cggggccgct tttttacatc cgtgaggtaa gtcttgccta
     1201 tggctatgtt attaaaatta cctgccggat caatctgtcc agggcggaag aactcctttg
     1261 gcctcagatt gggaagtatt tcagtagcta cgcgggcgaa tccagtattc attaaagagc
     1321 gatctaacca aaggctctcg attcgggtca gccccaatgg cgcgggatag cggcagatgc
     1381 tttgtccgat tgcagaggca aagtacaaat tgggagcgtg ggtagctcgt gccagcaagt
     1441 aac
//
LOCUS       DBNHOH010000486         1686 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_348815, whole genome shotgun sequence.
ACCESSION   DBNHOH010000486 DBNHOH010000000
VERSION     DBNHOH010000486.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_348815"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..757)
                     /locus_tag="AC16V7_08465"
     CDS             complement(197..757)
                     /locus_tag="AC16V7_08465"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6257194.1"
                     /translation="MITFRELAESDLSRIAEIDRSEVIHIGYEVREGALAEMDVMWDT
                     PNFIAEGEGEHTVTGEIEFCRSHMARNAIVIGGFDGEALIAIGILTPNIRPEMAQLAY
                     LHVSREYRRKGIGAAITRRLLERASALGSKRVYVSAVPSESAVGFYKSFGFDLIVEPL
                     PELYELEPDDIHMVLELDASDSIGSA"
     gene            complement(866..1291)
                     /locus_tag="AC16V7_08470"
     CDS             complement(866..1291)
                     /locus_tag="AC16V7_08470"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR6257195.1"
                     /translation="MSTEENKRIARRFYEEVVSTGNVSLLDELISPGYEEVMDGVRHP
                     VGLEGAKAHVVGVRQTYPDLKIAVNLQIAEGDWVASCITATGTHMGSWLGIKPTRKAV
                     TFTGVNVDRISGGKIIEHGGAANMLGPLLDIGALKVVSE"
     gene            complement(1497..>1686)
                     /locus_tag="AC16V7_08475"
     CDS             complement(1497..>1686)
                     /locus_tag="AC16V7_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257196.1"
                     /translation="DYNTVYALRRDGSMWVWSFVSGGEWGLFALVFATCTGAATLFAL
                     ALVVILFSGVWKRRSRQS"
BASE COUNT          411 a          493 c          389 g          393 t
ORIGIN      
        1 aagcgtaacc ctactgtatg tcattctgag gagtgaagcg acgaagaata tcgggttttg
       61 ctcctttcct ctatacttta ctccttaagc cgctgaacga tttgcgttac ctacgctagg
      121 gaaagacggc gaagccgtcc agccagaaaa aggctgaaaa cggcgcagaa tccccaatgt
      181 caggtgtcgg ccggtgttag gcagagccga tcgagtctga tgcatccaac tccaagacca
      241 tgtgtatgtc gtcgggctca agctcataca gctcgggaag cggctctaca atcaggtcaa
      301 acccaaatga tttgtagaag cctactgcgg attccgaagg taccgccgag acataaactc
      361 gcttcgatcc cagagcgctg gctcgctcaa gaagccggcg tgtaatcgca gctcctatac
      421 cttttcgacg gtactctctg ctaacgtgca gatatgccag ttgggccatt tcgggacgaa
      481 tatttggcgt gagaattccg attgcaataa gtgcctctcc gtcaaatcct ccgattacaa
      541 ttgcatttct tgccatgtga cttctacaaa actcgatctc tccggtaaca gtgtgctcgc
      601 cttctccctc tgcaataaag ttgggcgtat cccacatgac gtccatctct gctagagctc
      661 cctcgcggac ttcgtacccg atatgaataa cctcagatcg atcgatttct gcaattcttg
      721 agaggtcgga ttcagccagt tccctaaatg tgatcatgtc taaagatcca tatcagcatc
      781 aacggagctc tgcctaacgt ctgagctatg cggcggctcg taagggccat ccactgcagc
      841 gaattgttcg gcgtctccac acatgctact cgctgaccac cttgagcgct ccaatgtcga
      901 gtagaggccc aagcatgttg gcggcaccac cgtgctcaat gatctttccg ccactgattc
      961 ggtctacgtt tacaccggtg aaggtaaccg cttttcgtgt gggctttatg ccaagccagg
     1021 accccatgtg cgtgccagtt gccgtgatgc aactagcaac ccaatctccc tcggcaattt
     1081 gcaggttcac tgcgatcttt agatcagggt aagtctggcg aacgcctacg acatgggcct
     1141 ttgcaccttc caacccgaca gggtgcctta cgccatccat aacctcttcg tatcctgggg
     1201 atatcaattc atccaacagg gatacgtttc ctgtgctgac cacctcttca tagaagcggc
     1261 gcgcgattcg cttgttttct tccgtagaca tatcgagttc tccgaatttg acgcctaacg
     1321 gctgcgagct cagcagccag gaaatcgcca atcgacagca aatctcctcg catccctggt
     1381 ccgctgaaac cagtgttggg cagcgcccca gattgggtaa cactgcagat tacgcgacca
     1441 agtcaattgt aacgccaaca ccagcaagac cggatcgact aaatgagatc accactttaa
     1501 gactgacgag accgccgttt ccacactccg gagaaaagaa taaccaccag ggctagagca
     1561 aataacgtgg ctgctcccgt gcatgtcgca aataccagcg cgaacaatcc ccactctccg
     1621 ccagacacaa aactccacac ccacatactc ccgtcgcggc gaagcgcata gaccgtgttg
     1681 taatct
//
LOCUS       DBNHOH010000487         6988 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1759383, whole genome shotgun sequence.
ACCESSION   DBNHOH010000487 DBNHOH010000000
VERSION     DBNHOH010000487.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6988)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6988)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6988
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1759383"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="AC16V7_08480"
     CDS             complement(<1..239)
                     /locus_tag="AC16V7_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257197.1"
                     /translation="MHQMGFRTYLGVFLIALSLLMLEVTLTRIFSLILFYHFAVLSVS
                     LALFGSAAAGVMVYLFPAFFRKENSDTMLALLALI"
     gene            390..1466
                     /locus_tag="AC16V7_08485"
     CDS             390..1466
                     /locus_tag="AC16V7_08485"
                     /inference="COORDINATES: protein motif:HMM:NF033591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HIR6257198.1"
                     /translation="MPINTLYHTWFQRIQELRPGQRITQMRNIVWLIIGIYQSRSVCL
                     SRIAGKIPGTAKLLSTTRRLSRLLDNPAIRVREWYEPIAREWLETQYRNLGEIHLMVD
                     GTKIGFGHQLLIVSLAYRKRAIPIAWTWVKHVKGHSTASKQLALLAYVRKLIPGRAAV
                     FLVGDCEFGSIAVLRQLDRWCWLYVLRQKSDTLLWFNETSDWQPFGSFIQKPGQSIWL
                     GKGCLTAKEIYPVNLLIHWKVGEKDPWCLATNLPDRGIALRFYRYRMWIDEMFGDLKK
                     HGFDLEHTMLRHFDRLSRLTLAVALLYVWLISIGTRTIRNGLRPLVDRAERRDLSIFQ
                     IGLRYIDRCVTNALHFPVILCSYR"
     gene            1531..2244
                     /locus_tag="AC16V7_08490"
     CDS             1531..2244
                     /locus_tag="AC16V7_08490"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR6257199.1"
                     /translation="MDPITLVIIVIASYLLGAISFARLIPRIVDPKADLSDVSLPVEN
                     SEEKMPMLAMGANTASIKYGPKVGCSIGLLDIFKVFLPTLLVRVLYPDAPYYLAAAIA
                     GFIGHCWPVYYQFKGGRGISAFYGALFAIDPLGTVVVASTAMFIGMVLLKELLVAYTG
                     GVLLTVVWFAITRGWDFHLAYALVINVLFVVAMIPELKMIREIRQKYGREDMRNSMST
                     FPMGQAMLKLMDALKIQRK"
     gene            2432..4651
                     /locus_tag="AC16V7_08495"
     CDS             2432..4651
                     /locus_tag="AC16V7_08495"
                     /inference="COORDINATES: protein motif:HMM:NF013491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR6257200.1"
                     /translation="MSKELEQIIKPERSYAVRSSANFEDNNQRSFAGQFTSLLNVRGV
                     DHVSQAVQSIWEASESEGVQVYLKQSMQPKDRLLMAVIIQEMIEPVISGVSFSRNPIT
                     GAEEIIVEAVRGEGSQLMQTGITPMRWVSRWDIMLDKPDGESQLNRLIEQIVKETNQI
                     AILLRRDIDLEWVYDGRSLYWVQLRDITSLQEGDVYNNQIAREMTPGLIKPMVWSISI
                     PIHTGQWVRILNELVSNTQIEPARLVKAFHYRAYYNMGQFGRVFASLGMPRDSLERMM
                     GVLPPGVSRPKFMPGLSFLPKAPQVIRFILDKLRFGHRAERDYPILHQEARAFSGPLT
                     AYDHPEDLLERVDQIVCLQEKISYNTIMSILLMQIYNGLLRSHLKNRGVDPSQFDLTD
                     RWESMQAFDPNLHLANLHVQYQTLEPQLQHELKQGNLQALELEASLSDFRRDFYKFLD
                     RFGHLSDSTVDFTSIPWRESPDLILRLVCDFSPRDKQSVSKIGLQDLKTKRLSFFYLR
                     FIYQRARQFSLFREMYSSLYTFTLMLLRDHFHALGTILTVNGLLPSWENIFLLYMEEL
                     RFWVKGDADGKDFAALVTQRNDEMERSRHAQVPDVIRGDTIPPLIPANAQKLQGIPTS
                     GGYYAGRVKVIHGLNEFHKLEPGDVLVIPYSDVGWTPLFAHAGAVIAESGGILSHSSI
                     IAREYNIPAVVSVPTAMNLPDGSLVEVDGYKGLVYLNDGSQPARAVQEGATDASSAN"
     gene            4632..5381
                     /locus_tag="AC16V7_08500"
     CDS             4632..5381
                     /locus_tag="AC16V7_08500"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019544901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6257201.1"
                     /translation="MPVLQTNDLKKQYQMGTKRVDALAGVDFLVEKGEFVAIMGPSGS
                     GKSTLLHLVGGLDQPSGGEVYLAGARFSQLKDKKVTLLRRRNIGFVFQFFNLLPTLTA
                     EENITLPLLIDGKNLKGYQQRINTLLQLTGLSDRRWHKPEQLSGGEQQRVALARALVT
                     EPAIVLADEPTGNLDSKTGTAIMELLRKSCDELEQTIVVVTHDPRAAMYADRVVFLRD
                     GHIDGELRFLPLTPLTDKLHQIIERMTKLGE"
     gene            5393..>6988
                     /locus_tag="AC16V7_08505"
     CDS             5393..>6988
                     /locus_tag="AC16V7_08505"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6257202.1"
                     /translation="MNLLRLLSLRSLTTRPMRVLLSTFGIVLGVAAIIGIGVTNQTAL
                     NSVTQLFENTSGKADLIITSREADGRGIPETTAQRMEKFSGVFAALPSLQVQTVLANN
                     KQSDDISLGFFGTSLGGLLLYGIDPLRDSQARDYSIVAGRFLSPDLLADEVVLVRSYA
                     DQNELEIGKYIQFVAAGQVEKLRIVGLIEREGPGQLNNGAFGVMPLGTAQRLFDRSDE
                     IDQVDLVMSPGLSSDQLESERLRLLSYLGDSYSVIYPASQGKRMTQMLSNYQIGLNFL
                     SAMALFVGAFLIYNAFSMTVVERTREFGMLRTLGMTRLQVAFQVLVEAFSMGAVGSLL
                     GICLGLLMARGLSQLMAVIMAQEIPQAQAPQDLMVIAFTIGVLVAVAAALLPALRAGR
                     VSPLEALRVRVLANQGWLIRYGWTAGIALLVAAGWGLVFNPFPNDPQFRFGSLMVFAL
                     FLGGTLVIPISFSVWERTARPLMIVLFGRVGQLGSRNIQRSRLRTTLTVAALMIGVAM
                     IVIVWVMTDSFKGDLDEWLKGYIG"
BASE COUNT         1679 a         1695 c         1829 g         1785 t
ORIGIN      
        1 taaatcagcg ccaaaagcgc gagcatagta tctgaatttt cttttcgaaa aaaagctgga
       61 aagagataga ccatcacgcc ggctgccgct gatccaaata gagcaagtga aaccgataga
      121 accgcaaaat gataaaacaa aatcagtgaa aaaatacgcg ttagcgttac ctcaagcatc
      181 aataaagaca aagctataag gaatacgccc aagtaggtcc tgaaacccat ttgatgcatt
      241 ttgacaacct ccaaagcttt tttacggcgt tattaatact tgtattctac ttctagccac
      301 ctgacacttt aacaaaagaa tagttgatcg acaggatcga accggttaaa gtagttcttt
      361 ccacagaatt cacttaccgg aggagaccca tgccgatcaa cacgctctat catacttggt
      421 ttcagcggat tcaggaattg cgtcctgggc aacggattac ccaaatgcgt aacatcgtct
      481 ggctgatcat cggcatctat caaagccgct cggtttgttt gagccgcatc gctgggaaga
      541 tccccgggac ggccaaactg ctgagcacaa cccggcgctt gagtagattg ttggataatc
      601 ccgctattcg agtgcgggaa tggtacgaac cgatcgctcg tgaatggctg gaaactcagt
      661 accggaacct gggagaaatc catttgatgg ttgatggtac caagattggt ttcgggcatc
      721 aattgttgat cgtcagtctg gcttatcgca aacgagctat cccaatcgct tggacctggg
      781 tcaagcacgt caaggggcac agcacggcca gcaagcagtt ggctttgttg gcctatgttc
      841 gcaagctgat cccaggtaga gcagctgttt ttctggtcgg tgactgcgaa ttcggctcta
      901 tagccgtctt gcgacaattg gatcgctggt gctggctcta tgttttgcgt cagaaatccg
      961 atactttgct ctggttcaat gaaaccagtg attggcagcc attcggtagc ttcattcaga
     1021 aacccggtca aagtatctgg ctgggaaaag gctgcctgac cgccaaggag atttatccgg
     1081 tcaatttgct gatccattgg aaagtcggcg agaaagaccc ctggtgtctg gctactaatc
     1141 tgccagatcg gggcatcgcc ttgcgatttt atcgctacag aatgtggatc gatgagatgt
     1201 ttggtgattt gaaaaaacat ggttttgacc tggagcatac gatgttacgt cactttgatc
     1261 gcttgtctcg gctgacctta gcggtggcct tgctttatgt ttggctgatc tcgatcggaa
     1321 ctcgcaccat acgcaatggg ctgcgccctt tggtcgatcg tgcagagcgt cgtgatcttt
     1381 ctatcttcca aatcggtttg cgctatatag atcggtgtgt gaccaatgcg cttcactttc
     1441 ctgttatcct atgttcttac cgctgagagc aaactgtcag gtgactagat attaatagat
     1501 aaggttaatt attggaaaag agggagagaa atggatccaa taacactcgt tattatagtg
     1561 atagccagtt atctcctggg cgcaatttct ttcgctcgtc tgattccccg gattgtcgat
     1621 cccaaagctg atcttagcga tgtctcgttg ccagtcgaaa atagcgaaga aaagatgcct
     1681 atgcttgcaa tgggcgccaa cacagcatca atcaagtatg ggcccaaggt tggttgcagt
     1741 ataggactgc tggatatttt caaagtattc ttacctacat tgctggttcg ggtgctttat
     1801 ccggatgcac cctactatct agcggctgca atagcagggt ttatcggcca ctgctggcct
     1861 gtatattatc aattcaaggg tgggcggggc atttcggctt tttacggtgc cttgtttgct
     1921 atcgacccgc ttggcacagt agttgtcgcc agcacggcca tgtttatcgg gatggttctg
     1981 ctgaaggagt tgctggtggc ttatacgggt ggggtgcttt taaccgttgt ctggtttgca
     2041 atcacccgcg gctgggactt ccaccttgct tacgcactag taatcaatgt gctttttgtc
     2101 gtcgctatga tcccagagct gaagatgatc cgagaaattc ggcagaagta tggcagagag
     2161 gatatgcgta atagtatgag caccttccca atgggccagg caatgctcaa gttgatggat
     2221 gctcttaaaa ttcaacgcaa gtaaacgaga ttggggtggt atgaaatatc tagtatcgct
     2281 gcatacaaac aagttacctg acaatattgg gaacaaagct ctaaatctac atcgcctgtg
     2341 gcggaaaaaa ttcaatatac cgaggactta catatgtaca tggaatgcct tcctggactt
     2401 ccagagcctg ggtagcgttg ctgttgatgc cttgtcgaag gaattagagc agatcatcaa
     2461 gccagagcgg tcctacgctg tgcgctcatc ggccaatttc gaagacaaca atcagcgttc
     2521 atttgccgga caattcacca gcctgctaaa tgtacgcggt gtagatcatg tttcccaggc
     2581 cgtacaatcc atatgggagg cttccgagtc tgaaggggtt caagtctatc taaagcaaag
     2641 tatgcagccg aaggatcggc tcttaatggc agtgattata caagagatga ttgaaccggt
     2701 aatttcgggt gtgtcatttt cacgcaaccc gatcacgggc gcagaagaaa ttattgtgga
     2761 agcggtgcgt ggggaaggct cgcagttgat gcaaactggc attacgccaa tgcgttgggt
     2821 gagccgctgg gacatcatgc tggataagcc agatggcgag tcacagctaa acaggctaat
     2881 cgagcaaata gtgaaagaaa ccaaccagat tgcaatcctc ctgcggcggg atatagatct
     2941 cgagtgggtg tacgatgggc gctcattgta ttgggtacag ctgcgggata tcacttccct
     3001 tcaagaaggc gatgtctaca acaatcagat tgcacgtgag atgacgccag ggctcatcaa
     3061 acccatggtt tggtcgatct caatcccgat acatacaggc cagtgggtgc gcattctaaa
     3121 tgaattggtg agcaatactc aaattgagcc agcgcgcctt gtgaaagcct ttcattaccg
     3181 tgcgtactac aacatggggc agtttggccg cgtttttgcc agcctgggta tgccacgcga
     3241 ctccctggag cgcatgatgg gcgtactgcc gccgggtgtg agccgaccaa agtttatgcc
     3301 aggcctctca tttttgccaa aagcacctca ggtaatccgc ttcattttgg ataagctgcg
     3361 ctttgggcat cgcgccgagc gggattatcc tatcctgcac caggaagcgc gcgcattttc
     3421 cggaccactg acggcttatg atcatcccga agacctgcta gaacgggtag atcagatcgt
     3481 ttgtctgcag gagaagatca gctacaatac gatcatgagc atcttgctca tgcagattta
     3541 taatggcttg ctacgcagcc atttaaaaaa ccggggggtg gatcccagtc agtttgacct
     3601 cacggaccga tgggagagca tgcaagcctt cgacccgaac ctgcacctgg ccaatttaca
     3661 cgtccaatac cagacccttg agccgcagct tcagcacgaa ctaaaacaag gcaacttgca
     3721 agccctggaa ctggaagcta gcttatcgga ttttcgcaga gatttttaca aattccttga
     3781 ccgatttgga cacctgagcg atagcacggt ggattttact agcataccct ggcgcgaatc
     3841 gcctgatttg atcctgcgtc tagtctgtga tttcagtcca cgagataagc aatcggtcag
     3901 taaaattggc ttgcaggatc ttaagaccaa gcgcttgagt tttttttacc tgcgcttcat
     3961 ttaccagcgc gcccgtcagt tcagcctctt ccgcgaaatg tacagctctc tatatacctt
     4021 tactctcatg ttgctgcgcg accactttca cgcccttgga acaattctga cggttaatgg
     4081 cctgttgcct tcctgggaga atatattcct gctatacatg gaagaattgc gcttttgggt
     4141 gaaaggtgac gcagatggga aagactttgc cgctctggtt acgcaacgca atgacgagat
     4201 ggagcgtagt cggcatgcgc aagtccccga tgtgattcgc ggagacacga tacccccgct
     4261 cattcctgcc aatgcccaaa agctgcaagg catcccaaca tctgggggct attatgctgg
     4321 acgtgtgaaa gttatccacg ggctgaatga gtttcacaaa cttgagccag gcgacgtgct
     4381 cgtaattccc tactcagatg tggggtggac gcccctgttc gctcacgctg gagcagtcat
     4441 tgcagagtcg ggtggaatcc tgtcacacag ctctattatc gcccgcgaat ataacatccc
     4501 agccgtagtc tcggtcccca ctgctatgaa tctgccggat ggctcactgg tagaggtgga
     4561 cgggtacaaa gggctagtct acctgaatga cggcagccag ccagcccgcg cagttcaaga
     4621 gggagcaaca gatgccagtt ctgcaaacta atgacttaaa aaaacaatat caaatgggca
     4681 ccaaacgagt agacgctctg gcaggagttg atttcttggt ggaaaagggc gagtttgtcg
     4741 ctattatggg accctccggt agtggcaaat cgaccttatt gcacttggtc ggcgggctcg
     4801 accagccatc cggtggagaa gtctacctgg caggtgcccg gttctctcaa ctgaaggaca
     4861 agaaggtgac cctgttacgc agacgcaata ttggtttcgt cttccagttc ttcaatctgc
     4921 tgcccacact cactgcagag gagaacatca ccctgccgct gttaatcgat ggtaagaatc
     4981 ttaaaggata tcagcaacgg ataaatacac tgctacagtt aaccgggctg agcgaccgtc
     5041 gatggcacaa accagaacag ctctccggcg gcgagcagca gcgggtggcc ctggcgcgag
     5101 ccctggtcac tgagcccgct atcgtgctgg cagatgagcc caccggcaat cttgactcga
     5161 agaccggtac ggcgattatg gaattgttac gaaaatcctg cgatgagctg gaacagacta
     5221 tcgttgtagt aacccatgat cctcgtgcgg ctatgtatgc cgaccgagtc gtgtttctgc
     5281 gcgatgggca catagatggc gagctacgct ttttgccact taccccgctg accgataagc
     5341 ttcatcagat catcgagcgc atgactaaac tgggtgaata aatcatcttt tgatgaatct
     5401 cctgcgtttg ctttccctac gttccttaac cacccgccct atgcgggtct tgttaagcac
     5461 attcggcatc gtcctgggtg tagctgcaat tattggcatt ggcgtaacca accaaactgc
     5521 cttgaactcc gtcacgcagt tgtttgaaaa cacatctggg aaagccgacc tgatcattac
     5581 cagcagggag gccgatgggc gcggcatccc agaaaccact gcacagcgca tggagaagtt
     5641 ttctggtgtt tttgcagccc taccatctct gcaagtacag acagtgcttg ccaataacaa
     5701 gcaaagtgac gatatcagtt tgggcttctt cggtactagt ttgggaggat tgctgttata
     5761 tggcattgac ccacttcggg atagccaagc gcgcgactac agcattgtcg cagggcgatt
     5821 cctttcccct gatctattgg cggatgaggt ggtgttggtc aggtcatatg ccgaccaaaa
     5881 tgagctcgaa atcggcaaat atatccaatt tgttgccgcc gggcaagttg agaagctgcg
     5941 catcgttggt ttgatagaga gagaaggtcc gggacaattg aataatggcg ccttcggtgt
     6001 gatgcctctc ggcaccgccc aaagattgtt cgatcgcagc gacgagatcg atcaggtcga
     6061 cctggttatg tcgcctgggt tgtccagcga ccaactggag agcgagcgtc tgcgcctgtt
     6121 gtcttacctg ggtgactcat actctgtgat atatccagcc tcacagggga aacgcatgac
     6181 ccagatgctc agcaactacc aaattggttt aaatttcttg tccgctatgg cgcttttcgt
     6241 gggcgctttc ctgatctaca atgccttttc tatgactgtg gtcgagcgta cgcgcgaatt
     6301 tggcatgtta cgaacgcttg gcatgacacg cctacaggtg gcctttcagg tactggtgga
     6361 ggccttttca atgggcgccg tgggttcttt actaggtatt tgcttgggtc tactgatggc
     6421 acgtgggctt tcgcaactga tggctgtcat aatggcacaa gaaatccctc aggcccaggc
     6481 accgcaagac cttatggtga ttgcctttac tattggtgtg ctagtagcgg tagctgcagc
     6541 gttactcccc gccctgagag caggacgtgt ctctccactg gaagcgctgc gcgtgcgtgt
     6601 ccttgcgaat cagggctggc taatccgcta cggatggact gcagggattg ctctgttggt
     6661 tgcggctggg tggggattgg tgttcaatcc ctttcccaac gacccacagt ttcgttttgg
     6721 aagcctaatg gtatttgctc ttttcctggg tgggacgcta gttatcccta taagcttctc
     6781 tgtatgggag cgcaccgccc gcccgttgat gatcgttctg tttggacgcg ttggtcaatt
     6841 gggcagccga aatattcaac gctcacgctt gagaaccacc cttacggtcg ccgcattgat
     6901 gatcggtgtc gcaatgatag tgattgtttg ggtgatgacc gattccttca aaggagacct
     6961 ggatgagtgg ctaaagggct acatcggt
//
LOCUS       DBNHOH010000488         4369 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_605676, whole genome shotgun sequence.
ACCESSION   DBNHOH010000488 DBNHOH010000000
VERSION     DBNHOH010000488.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4369)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_605676"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="AC16V7_08510"
     CDS             <1..510
                     /locus_tag="AC16V7_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257203.1"
                     /translation="LCLEQHSQEAGTLRIRPTPLGYKFWQDVVGPAEEGTAIKSGQLE
                     GTEPIDSIQPTPLPTQVISQPASEGIIVNVWKKYPVIAPGQRQEIGVVVFRDNLPLAG
                     AHMALILTLDNGESVRFEMEPSGSDGQSYYLMEAMDAPLGEPIVYQVCVYYRPGEAAC
                     VQDSFLIWQ"
     gene            complement(497..1084)
                     /locus_tag="AC16V7_08515"
     CDS             complement(497..1084)
                     /locus_tag="AC16V7_08515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6257204.1"
                     /translation="MPKDDALRWNSRYLDDERYSTFAQPRAFLVKNAHFLPPKGLALD
                     VAMGLGGNAGFLLSRGLRVVGVDISSVAVHQASKRFAGLMAVVADLANYYLPPETFDV
                     ILNFYYLQRDLWSQYQLALRPGGILYIETLTQEMLQLQPNTDPAYLLAPGELRQAFAS
                     WEILVYQEGWEESRGGRHHPVASLVARKPHSLTAR"
     gene            complement(1152..2345)
                     /locus_tag="AC16V7_08520"
     CDS             complement(1152..2345)
                     /locus_tag="AC16V7_08520"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6257205.1"
                     /translation="MSNSQLSTLLNRYQQLIEISEDLASTLNLETLLNRIVHVAADLC
                     SAEAASILLYNESNHQLHFEAASNLEFPYMRGLMVPVETSIAGWIVTQRQPVIISDAQ
                     QDPRHFKHIGEVTSIQTNSLLGVPMITKDKVIGVLEAINKLSGQFSQEDQELLMALGS
                     QAAVAIENARLFQQSDLISELVHELRTPLTSLNAAAHLLQRPELGDEQRNAILNTLQT
                     EITRLLDMSTAYLDFARLESGRVQYHFKMIDIGALLEECARVIRGKVVEKEINFQLSL
                     LDGSATIHGDNDKLKQVILNLLSNAIKYTPSGGVIKLTGEILDEEIVIHVSDNGIGIP
                     QDSLPFIFEKFYRVPGVEQPIQGTGLGLFICKRIVHDHLGTIEVQSNFGDGTEFILHL
                     PLVKS"
     gene            complement(2338..3114)
                     /locus_tag="AC16V7_08525"
     CDS             complement(2338..3114)
                     /locus_tag="AC16V7_08525"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012966062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - histone deacetylase activity,
                     NAD-dependent [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family NAD-dependent protein deacylase"
                     /protein_id="HIR6257206.1"
                     /translation="MANISEKSQNGITSAADIIKDSHHAVVLTGAGISTPSGIPDFRS
                     QKSGLWQRYDPFEVASLSAFRQKPDKFFEWLHPLAVTIFNAQPNPAHVGIAQLESAGY
                     IKAVITQNIDRLHQRAGSKQVLEVHGSFDTLTCVACFHQVASEPHVQGYLAHAKIPYC
                     PDCGNVLKPDVILFGEQLPVHTWLSAVEASKKCDLMIVAGSSLEVLPVAGLPMRAVEN
                     GAHLIIVNETPTYIDVRADVVFWDDVAEIIPRIVREVLGV"
     gene            complement(3117..4265)
                     /locus_tag="AC16V7_08530"
     CDS             complement(3117..4265)
                     /locus_tag="AC16V7_08530"
                     /inference="COORDINATES: protein motif:HMM:NF018290.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpaE/TadZ family protein"
                     /protein_id="HIR6257207.1"
                     /translation="MAEKILLIDDDIDTLRLVGLMLQRQGFEVQVASNGPQALGMAKA
                     DPPNLIVLDVMMPEMDGYEVARNLRADPATGEIPIIMFTAKGDVDDKVAGFEAGVDDY
                     LTKPTQPRELIAHIRAVLARTAKRTAPLGPADGRAHLIAVLAARGGLGVTSVALNLGI
                     TLREDTKKDVVVAEFRPGQGTIGLELGYQNSESLNRLLKRRPNEISASEIEAELTSHP
                     LGTRLLLSSYQPRDSQLMASVANFEAITRHIAHTARYVIIDLGPALTPVADKVIKLCD
                     EIILVVEPVPQTVTHTKALVNDLFNLGVGEGKITPVLVNRVRSDVQLSWSQVQDQLGL
                     NIAVIFTPAPELAFQASMKNLPIVLQQRDSLTAQQYNKLAERITQRSK"
BASE COUNT         1044 a         1075 c         1151 g         1099 t
ORIGIN      
        1 ctgtgcttgg agcagcattc tcaggaagcc gggaccttgc gtattcgtcc aactccattg
       61 ggatacaaat tctggcaaga tgtagttggc ccagctgagg aagggacggc gattaagtcg
      121 ggacagcttg aaggaaccga accaatcgac tctattcaac ccacacctct tcccacccag
      181 gtgatttctc aaccggcatc cgagggtatt atcgtcaatg tatggaaaaa gtaccctgtg
      241 attgcaccag ggcagaggca ggaaatcggt gtggtagtat tccgagataa cctccctctg
      301 gcaggtgccc atatggcgct gatcctgact ttagataacg gggagtcggt ccgctttgag
      361 atggaaccca gtggtagtga tgggcagagt tactacctaa tggaagctat ggatgccccg
      421 ctcggtgagc caattgtgta ccaggtgtgc gtttactatc gcccgggcga agcggcgtgt
      481 gtgcaagaca gctttcttat ctggcagtga gcgaatgtgg ctttctagct accaggctgg
      541 caaccgggtg atgccgaccg cccctggatt cctcccagcc ttcttggtaa accagaatct
      601 cccagcttgc gaatgcctgg cgtagttcgc caggggcgag caggtaagcc gggtctgtat
      661 ttggctgtag ctgcagcatc tcttgggtga gggtttcaat atagagtatg ccccctggtc
      721 gcagagctaa ttgatattgc gaccataaat ccctttgcaa gtaataaaaa tttaagatga
      781 catcgaaagt ttcagggggc agataataat ttgccagatc tgcgacaacc gccatcaggc
      841 cggcaaaacg tttgctggct tggtgcactg caacggagga gatatccacc cccaccacac
      901 gcagaccacg gctgagcagg aagcccgcgt tgcctcccag ccccatggcg acatccaaag
      961 ccaaaccttt gggtggcaag aaatgagcgt tcttcaccaa aaaagcgcgg ggttgtgcaa
     1021 aggtggaata acgctcgtcg tccaggtagc gggagttcca acggagagcg tcatcttttg
     1081 gcatggaaag gatagttttt tagaagaaat tcagatcaag gatatttaca gagttgttca
     1141 cttgtgtgaa attatgactt caccaacggc agatgcaaga taaactcggt gccatctccg
     1201 aaattgcttt gtacttcaat tgtccccagg tggtcatgaa caatccgttt acagatgaac
     1261 agccccaatc cggtgccctg aattggctgc tccacaccag gcacccggta gaatttctca
     1321 aaaataaaag ggaggctgtc ctgtgggatg cctataccat tgtcgctaac atggatgacg
     1381 atttcttcat ccagaatctc cccggtcagt ttgatcactc ctccagaggg cgtgtacttg
     1441 atggcgttgc tcaacaggtt cagtatcact tgcttgagtt tgtcgttgtc accatgaatt
     1501 gttgccgatc catcaagaag acttaattgg aaattgatct ctttttccac gaccttccct
     1561 cgtattacac gagcacattc ttccaaaagc gcgcctatgt cgatcatttt gaaatgatat
     1621 tggacacgcc ctgactccag gcgggcaaag tccaggtaag ccgttgacat atctagcaag
     1681 cgggtgatct cggtttgaag tgtgttcagg atggcgttgc gctgctcatc gcctaactcc
     1741 ggtcgctgca acaggtgtgc ggctgcattc aaggatgtga gcggtgtgcg cagttcatgt
     1801 accagctcgg atattagatc ggattgttgg aagaggcggg cgttttcgat agcgacagca
     1861 gcctgtgaac ccagcgccat gaggagttcc tggtcttcct gagaaaattg ccctgagagc
     1921 ttgttgatcg cttcaagcac accgattacc ttatccttgg tgatcatcgg gacaccaagc
     1981 aaagaattgg tctgtatact ggttacctcg ccaatgtgct tgaagtgtct agggtcttgc
     2041 tgggcatcgg aaatgatcac tggttggcgt tgcgtgacga tccagcctgc gatgcttgtt
     2101 tcaacaggta ccatcaggcc gcgcatgtag ggaaattcca aatttgaggc agcttcaaaa
     2161 tggagctggt gattggattc attgtacaag aggatagaag ctgcttcagc agaacacaaa
     2221 tcagccgcca catgaacgat gcggttaagc aaagtttcca ggtttagtgt ggaggccaga
     2281 tcttctgaga tctcaataag ctgttggtaa cggttgagca gtgtactcag ttgagagtta
     2341 gacacccaat acctccctaa cgatgcgagg gatgatctcg gcaacgtcgt cccaaaatac
     2401 cacatcggcg cgtacgtcga tatatgttgg ggtttcattg acgatgatta agtgcgcccc
     2461 attttcaacg gcgcgcatgg gcagcccggc taccggtaag acctcgagcg aggaaccagc
     2521 gacaatcatt agatcgcact ttttactcgc ctccactgca cttagccatg tgtgtaccgg
     2581 gagttgctcg ccgaacagga tgacatccgg tttaagtacg ttgccacaat ctgggcaata
     2641 gggaattttt gcgtgggcca gataaccttg cacatggggt tccgacgcaa cttggtgaaa
     2701 gcaagccacg caggtcagcg tatcgaacga gccgtgcacc tccagaactt gcttggaacc
     2761 agcgcgctgg tgcaagcgat cgatgttctg ggtaataact gcttttatgt agccagcgct
     2821 ctccaactgg gctatcccga catgtgccgg attcggttga gcgttgaata ttgtgactgc
     2881 taatggatgc agccactcga agaacttgtc tggtttttgc cgaaacgcag ataatgaagc
     2941 cacctcgaaa ggatcatagc gttgccacaa gccactcttc tgcgatcgga aatcaggtat
     3001 tcccgatggg gtggaaatgc ctgcaccagt cagaacaacc gcatgatgac tgtctttaat
     3061 gatgtccgcc gcgctagtta taccgttttg gcttttctcg gaaatatttg ccatcttcac
     3121 ttgctgcgct gcgtgattct ctccgcaagc ttgttatact gctgcgctgt aaggctgtct
     3181 cgctgctgca agacaatggg aaggtttttc atcgaagcct gaaaggctaa ctctggcgca
     3241 ggggtgaata tcactgcgat atttagccct aattgatctt gtacctggct ccaggagagc
     3301 tggacatctg aacgcacgcg gttaaccagg actggtgtga tcttgccctc gcctactcca
     3361 agattgaata agtcgtttac caacgctttg gtatgggtaa cagtctgggg tacaggttct
     3421 acaaccagaa ttatctcgtc acatagctta atgactttat cagcaactgg cgtcaaagca
     3481 gggcctaaat cgatgatcac atagcgggcg gtatgggcga tgtgccgtgt gatggcttcg
     3541 aagttcgcca ctgaggccat gagctggctg tcacgcggtt ggtaggagga gagcagcagg
     3601 cgcgtaccca atggatgtga ggttaactct gcctcaatct cgcttgcgct aatttcattt
     3661 ggcctgcgtt tcaacaggcg gtttaagctt tcggaattct ggtagcctaa ttctaggcca
     3721 atggtgccct gaccaggacg gaattcggcc acgaccacat ctttctttgt gtcttcacgc
     3781 agcgtgatac ccaagtttaa ggcaacgctg gtcactccta aaccacctct ggctgcaagc
     3841 actgcgatca aatgggctct accatcagct gggccaaggg gagcggtgcg tttggcggtg
     3901 cgcgccagca cagcacggat atgggcgatt aactcgcgag gctgtgtggg tttggtaagg
     3961 taatcatcta cgccagcctc aaatccggcc actttatcat cgacatcacc cttggcggtg
     4021 aacatgatga tcgggatttc cccagtagca gggtcagctc gaaggttgcg cgctacttca
     4081 tagccgtcca tttctggcat catcacatcc aaaacgatca gattgggcgg atcagcctta
     4141 gccattccaa gcgcctgcgg accattgctg gcaacttgga cttcgaaacc ctgtctctga
     4201 agcatcaatc cgaccaggcg taatgtgtct atgtcgtcgt ctataagcag aatcttttca
     4261 gccatttgat cttccttcct gcgtgtgcat taagtgtcag tctagcataa aggcaaggtc
     4321 aaagcaagag gctttcgatt ttattagctt gaatagaata taattctta
//
LOCUS       DBNHOH010000489         5489 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_477584, whole genome shotgun sequence.
ACCESSION   DBNHOH010000489 DBNHOH010000000
VERSION     DBNHOH010000489.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5489)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5489)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5489
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_477584"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="AC16V7_08535"
     CDS             <1..860
                     /locus_tag="AC16V7_08535"
                     /inference="COORDINATES: protein motif:HMM:NF013927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HIR6257208.1"
                     /translation="IVDYFESRDVLKVADFQALHVDFTCLPGAEFVASLDDFKSDDPD
                     VKLALKILRAWDGKLTADSVAGALYEVARYHIVRNLMEPALGEALTFHWMGQGFHPVL
                     LPSNEFYGHDTLVMLRLLSNPDSWWVVQAGGRHKVLSQGLAQAVRWLRAQLGADTQSW
                     QWGRIHRAIFPHPLGLQKPLDRVFNCGPFPIGGDTDTPCQTATHPNAPYDSNSWAPSF
                     RQIVDMSDLSQSLVIIPSGQSGQLGSPHYDDMIQPWLKGEYIPMLWTREQVESQAEGK
                     LDLKPSLPT"
     gene            1277..4126
                     /locus_tag="AC16V7_08540"
     CDS             1277..4126
                     /locus_tag="AC16V7_08540"
                     /inference="COORDINATES: protein motif:HMM:NF037660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR6257209.1"
                     /translation="MTTSTLFSPSDLPTGTLTFLFTDVEGSTRLWELYPEEMRVAMAR
                     HDELIESCVVQSGGKVVRPRGEGDSRFAVFQYASDAAIAAIAIQRKFFTASWPFSEPL
                     RVRMALHTGEADLREGDYYGSAVNRCARLRGAAHGGQILISQTTQLLITEALPDGADL
                     LDLGEHKLKDLHRSERIYQIIIQGLPADFPPLKTPDSFLTNLPIPRNQLIGRNLEMET
                     ISDLLLLQDVALVTLTGTGGTGKSRLGLQIALDLRENFEDGVYLVALESIRDPKLVIP
                     TIAETLGVRESLGSRPISDILKEFLLNKQMLLLLDNFEQVVAAAPSVGDLLEACPRLK
                     ILVTSRTPLRLRAEKELPVSPLMVPTLEEYSNRSSLSQYAAVELFIQRAAAIKPDFAV
                     TNANAPAVAEICHRLDGLPLAIELAAARIKLLTPQGILTRLENRFELLRGGTRDLPER
                     QRTLRGTIDWSYNLLNELEKKLFQRLSIFIGGWTLEAAEMVCDIDGDLTQTLYDVLES
                     LIDNSLVIQIQDSEEGPRFGMLSTIHEYGRERLMESQEADTIHYQHAQYYLNFVQKVE
                     PLIRSAERMRWQQVMQQELGNVRGVLEWICRTGKCVEIGQHIVITLGIYWMISGYIAE
                     GQQWCNLITALSDESTPISIRAGLLGVAGEIAWMQGDHSSSKTNLDESLELLHRLDDK
                     TLEDKHILATVTMIRGLLAATSRDYETATTLYQRVIELCEEINDQWLEVITISWLGDI
                     ALYKNDSDRAMALYNQSIKLARQQGDPWSLMPSLMSSGQIALVKGDLANARSILNEAE
                     ALLRNTGDHWSLSWVLNDLGHIMLMEGKLDQAVSNFVEGITLAKVMGNLRVLVISLAG
                     TAALIAKRLKDLPDAHRQDSAELAIAGRLCGATLPYIDRPGVFSWFDSKLLYDADIAQ
                     VQSLLDKDLWDKTSSEGQSMSFEQVIDLALQSLKE"
     gene            complement(4303..4647)
                     /gene="rsfS"
                     /locus_tag="AC16V7_08545"
     CDS             complement(4303..4647)
                     /gene="rsfS"
                     /locus_tag="AC16V7_08545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIR6257210.1"
                     /translation="MDTLEVSRTIVNALEEKKGEDILLLDIQGLAPFADYFVVCSGSS
                     ERMLKALADAAVDQVRKQYQVRGRIEGEPREGWVLVDFGDIILHLFSPDRRDYYRLEE
                     LWVEGKVLLRLQ"
     gene            complement(4728..5327)
                     /gene="thpR"
                     /locus_tag="AC16V7_08550"
     CDS             complement(4728..5327)
                     /gene="thpR"
                     /locus_tag="AC16V7_08550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HIR6257211.1"
                     /translation="MSVIRAFIAIDLSAEIQEKLEQISRQLRQQLRGSPIRWVHTQNI
                     HLTLRFLGNVSLANLEVLENILLSEASHHASFEISVGELSAFPSTRRPRVILVRVQAP
                     QEMGQLQHSLEEAIRRLGYAPEEREFKPHLTLGRVSRSANSTEVQQIGQVLDNTNIGF
                     VGAMHVRSVNLYRSDLQPSGAVYSCLFTANLLEGEHLGS"
BASE COUNT         1406 a         1402 c         1378 g         1303 t
ORIGIN      
        1 gcattgtaga ttactttgag agcagggatg tgctcaaggt ggcggatttc caggctttgc
       61 acgtagactt tacctgcctg cctggggctg aattcgtcgc cagcctggac gattttaaaa
      121 gcgacgaccc cgacgtgaaa ttggcattaa agatcctacg cgcctgggat ggcaaactga
      181 ccgccgattc agtggcaggt gcactttacg aggtagctcg ctaccatatc gttcgcaact
      241 tgatggagcc agccttgggg gaggcattga ccttccactg gatgggacag ggtttccacc
      301 cggtgctctt gccttctaac gaattttacg gtcacgatac gctggtcatg ctgcgactgt
      361 tatccaaccc agattcgtgg tgggtagtgc aagctggtgg gcgccataag gtcttgagcc
      421 agggtttagc tcaggcggtc cgctggcttc gcgctcagct gggagcggac acgcaatcct
      481 ggcaatgggg caggattcac cgggcgattt tcccccaccc attgggtctg caaaaaccgc
      541 ttgaccgggt cttcaactgc ggtccttttc ccatcggcgg ggacacggat accccctgcc
      601 agaccgccac ccatcccaac gctccttacg acagcaactc ctgggcgcca tccttccgcc
      661 agatcgtcga tatgagcgac ctttctcaat cgctggtcat catcccatca gggcagtctg
      721 ggcaattggg tagcccgcat tatgatgata tgatccaacc ctggctgaag ggcgaatata
      781 tccccatgct gtggacgagg gaacaggtgg aaagccaagc cgaagggaag ctagatctca
      841 agccatccct cccgacttag cgctcgcagg gattggtcca cgccaaacac attcgcctcg
      901 aggcagaaag gcataatcaa gatcgcctgg cttgtatgga ctatccatcg ccggggcatg
      961 ctttactgaa tatccccaag gtgcgaatta ggggttgagc tcataaccca tagcttttga
     1021 ttctaaccca agacccgcta ctctgaaaaa gacaccacaa agttcctttg attcgttata
     1081 atagtgcgaa atctggattc tgagtaaggg gatagcaatc gttgcacatg aggtccccat
     1141 ggcgatagtt cgatcactgg gaactgcagg gttttgacca tcgacaagaa gaaatttctg
     1201 cgccatatcc atgaatatgc ctcgaaaata ttctaaggga gagtggtggt tgatccgaaa
     1261 tggttaggcc aaattgatga ccacatcgac tctattctca ccttccgatt tgccgaccgg
     1321 gacgctgaca tttctattta ccgatgtaga aggcagcacg cgcctatggg agttataccc
     1381 cgaggagatg cgggtggcca tggcgcgtca tgatgaattg attgagtcct gtgtagtcca
     1441 gtctgggggc aaggtggtcc gaccccgcgg agaaggcgac agccgctttg ccgtctttca
     1501 gtacgcttca gacgccgcaa ttgctgccat tgctattcag cgtaaatttt tcacagcatc
     1561 atggcctttc tctgaacctt tgcgtgtgcg catggctctg catacgggtg aagccgacct
     1621 acgtgagggc gattattacg gaagcgcggt aaaccgctgt gccaggttgc ggggcgccgc
     1681 tcacggcgga caaatcctta tatctcagac cacacaatta ttgataacag aggcattgcc
     1741 tgatggtgca gacctgcttg acctgggtga acataaactc aaagatctcc accgttctga
     1801 acgcatatat cagatcatca tccagggatt acccgctgat ttcccaccac taaaaacacc
     1861 agatagcttt ctcacgaacc taccaatacc gcgtaaccag ctgatcgggc gcaatttaga
     1921 gatggaaacg atatccgatc tgctcttgct ccaggatgtc gccctggtca cgttgaccgg
     1981 aacaggaggg acaggtaaat cccgcctggg cttacaaatc gcgctggatt tgcgtgagaa
     2041 ctttgaagat ggggtttacc tggtagcgct ggaatcgatc cgcgacccga aactggtgat
     2101 tccaaccatt gccgagaccc tgggtgttcg cgaatccctt ggcagccggc caataagcga
     2161 cattctcaaa gaatttttac ttaacaaaca gatgctcttg ctgctggata attttgagca
     2221 agtagtggca gctgcaccct ctgtagggga cctactcgaa gcctgcccac ggttgaagat
     2281 tttagtaacc agccggacac cattacgcct acgggcagag aaggaacttc cagtttcacc
     2341 gctgatggtt ccaaccctgg aggaatattc caacaggagc agtctctcac aatatgctgc
     2401 agtcgagcta tttattcaac gcgcggcagc gataaagccg gatttcgcag taaccaatgc
     2461 caatgcaccc gccgtggctg aaatctgcca ccgcctggat ggattgccgt tagcaatcga
     2521 gcttgcagca gcgcggatca agctgctcac cccccaaggg atactcaccc gtctggaaaa
     2581 ccgctttgag ctcctacgcg gcgggacgcg tgatcttccg gagcgacaac gcaccttgcg
     2641 cggcactatc gactggagct ataaccttct caatgaattg gagaagaagc ttttccagcg
     2701 gctatcaatt tttatcggcg gatggacgct tgaagcagca gaaatggtct gcgacatcga
     2761 tggagactta acccagactc tgtatgatgt gctcgagtcg ttgatcgaca atagcttagt
     2821 gattcagatt caggatagcg aggaaggccc gcgctttggg atgctcagca caatccatga
     2881 atacgggcgc gagcgcctga tggagagtca ggaggctgat accatacatt accagcatgc
     2941 ccagtattat ctcaattttg tccaaaaggt tgaacccctc atccggtcgg ctgagcgtat
     3001 gcggtggcaa caagttatgc agcaggaact cggaaacgta cgcggggttt tggaatggat
     3061 ctgcagaact gggaaatgcg tcgagatcgg acaacatatt gtcatcacgc tggggatata
     3121 ttggatgata agtggctata tagccgaagg acaacaatgg tgtaatctaa tcacagcgct
     3181 gagtgatgaa tcgacaccaa tatcgatccg cgccggcttg ttgggagttg cgggtgagat
     3241 tgcctggatg caaggcgacc attcctcatc gaagactaat ctggatgaga gcctggaact
     3301 gctacatagg ctggatgaca aaacgttgga agacaaacac atattagcca ccgtcacgat
     3361 gatccgaggc ttattggcgg caacctctcg cgattacgaa acagcaacta cgttgtatca
     3421 aagggtcatt gaactttgcg aagaaattaa tgaccaatgg ctagaagtta taacaatatc
     3481 ctggttaggt gatatcgcat tgtataagaa tgatagcgat cgcgccatgg ctctctacaa
     3541 tcaaagtatc aaactggcac gacaacaagg cgacccctgg agtctgatgc cctccctgat
     3601 gtcatccggg caaattgccc tggtgaaagg ggatcttgcc aatgcccgct caatcctcaa
     3661 tgaggccgaa gctctcctac gcaatacagg tgatcattgg agtctttcat gggttttgaa
     3721 tgacctaggc catatcatgc ttatggaagg caagcttgac caagcagtta gtaattttgt
     3781 ggaagggata accctagcaa aggtaatggg taacctgagg gtcttggtca tatccctggc
     3841 agggacggct gccctaattg ccaagcgctt gaaggatctt ccagatgctc accgtcagga
     3901 ctcagccgag ctagccatag ccgggcgtct atgcggagcc accttgccat acattgatag
     3961 gccgggggtt tttagttggt ttgactcaaa attgctttat gatgcagaca tcgctcaggt
     4021 gcaatcttta ttggacaaag atttatggga caagacctct tccgagggtc aaagtatgtc
     4081 gtttgagcag gtgattgatt tagcactaca atcgcttaaa gaataatgga tattggcagc
     4141 ggaacttgat aacttgcctt aactcactgc acaattattg ggatatccca atacctgaca
     4201 ggcggtatgg atatattgcc tgggatgagg gaacacaaaa ttggggaaat cctttttatt
     4261 gaataacccc cacttatatc tatctaatta ttgaggggaa gcctactgca gacgcaacag
     4321 cacctttcct tctacccaga gctcttccag acggtagtaa tccctgcggt cgggagagaa
     4381 aaggtgcaga atgatatccc caaaatccac cagcacccag ccctcgcgcg gctcaccctc
     4441 aatacgccct cgcacttggt attgtttgcg aacctggtca accgccgcgt cggcaagggc
     4501 cttcaacatc cgctcgcttg acccagaaca aaccacaaaa tagtccgcaa atggagccag
     4561 cccttgaata tccaaaagca ggatatcttc ccctttcttc tcttcaagtg catttacaat
     4621 cgttcgcgaa acttctaaag tatccagata tcacctctca aattttgaaa taaaagatca
     4681 aagttgaatc ggggaattga gggggtttca cccatccaaa tcctttctta acttcccaag
     4741 tgctcaccct ctaataaatt agcagtaaac aagcaagaat atacagcgcc actgggttgc
     4801 aaatcgctcc gatagagatt tacagatcgc acatgcattg caccgacaaa accgatattg
     4861 gtgttatcca atacctgccc aatttgctgc acctctgtcg agttggcgct gcgcgaaacc
     4921 cttcccaggg ttagatgtgg cttgaattcg cgctcttcgg gtgcataacc caggcgacgg
     4981 atggcttctt cgaggctatg ctgcaactga cccatttcct ggggcgcttg tactcgaacc
     5041 aatatgaccc gtgggcggcg agttgatgga aaagcgctca attctcccac actgatctca
     5101 aacgaggcat ggtgcgaggc ttcagacagg aggatatttt ccagaacctc cagattagcc
     5161 aaggacacat tccccaggaa cctaagcgtt aggtgaatgt tttgtgtatg cacccagcgg
     5221 atagggctcc ccctcaattg ctgtcgcaac tggcgagata tctgctccag cttttcctgg
     5281 atctcagccg acaagtctat agcgataaaa gcacgtatca ccgacattaa tattattcct
     5341 gctcacaacc tccacgacca cgctcctgat tgacgtggcc gttatcaatt ggtatctgtt
     5401 ttcatagttg aaaatagcac aagatcagcc agaatgcaag aggagaaact atctttttca
     5461 gggctttctt aaagtaggaa aaagagaga
//
LOCUS       DBNHOH010000490         2786 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_348973, whole genome shotgun sequence.
ACCESSION   DBNHOH010000490 DBNHOH010000000
VERSION     DBNHOH010000490.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_348973"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..1016
                     /locus_tag="AC16V7_08555"
     CDS             219..1016
                     /locus_tag="AC16V7_08555"
                     /inference="COORDINATES: protein motif:HMM:NF013323.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HIR6257212.1"
                     /translation="MDKLITLEQAVGLVRSGDMLALGGITLYRRPIAFVRALIRRFFG
                     EAEPGDLTLLAFTAGLESDLLVGSGMVSRIRTCYFGLEIFGLAPMFTYFANRGEIEIM
                     EESEASLAFGLRAQMAGVGFMPGLGWLGTDLPKLRPDVLTVTDPYNGEELIAFPAIHP
                     DVAVIHALQADPHGNAQIGDNKGIDEELALTARTVIVTAEEIVPEMEKANLVAPLVHY
                     IVHASHGALPTSCHPLYPLDGQALLAYVDKVSEPESCRAFLESWIAE"
     gene            1246..2490
                     /locus_tag="AC16V7_08560"
     CDS             1246..2490
                     /locus_tag="AC16V7_08560"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR6257213.1"
                     /translation="MKSKALVTFISVIIAGILLTGACSAGFVAGRVFNPPEQILALLP
                     GQEISPPVAQEESGTPQELEELFVPFWQAWDLVHDQYVEQPVDDEVLMRGAIEGMLDS
                     LGDPHTSYLNPEQYQSLNTQLQGDESYEGIGAWVDTTSDFLTIISPIPGSPAEKAGLR
                     TGDKVIAVDGEDMTGIDGELVRQRVIGPAGSTVNLTILRTGQDPFDVQITRAEITIPS
                     VDYRMLEDDIAYVRVLIFADNTKDQLQDALKDLMSQNPQGMILDLRGNGGGYLDSAID
                     VASEFISEGVIAYEEYGDGTRDTYDARRGGLATDIPMVVLINEGSASASEIVAGAIQD
                     LGRGQLIGMTSFGKGSVQIPTALRNQQGAVRITVARWLTPKERTIHGKGLTPDVEVPV
                     TEEDFANNRDPQLEKAVEIISK"
     gene            2580..>2786
                     /locus_tag="AC16V7_08565"
     CDS             2580..>2786
                     /locus_tag="AC16V7_08565"
                     /inference="COORDINATES: protein motif:HMM:NF016206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HIR6257214.1"
                     /translation="MFFFYDYRYLMFMLPAFLLMLAVQWYVNSAYNKWGRVPARSRMS
                     GAQAAQRLIERGGLYDVKIEGVQGN"
BASE COUNT          673 a          721 c          742 g          650 t
ORIGIN      
        1 tctcgacgac tcacctcgaa accttgctcc tgcccggaag ctaggttttt tcacgatctt
       61 gattggggac gacgacatcc acaccgcagc agacctcgcc atgcgccttc cccacgactt
      121 gcctaggcta atgccagagt tgtggcagga aaaccaccgc catccaggat aagcacgcgt
      181 tgagcaaggg ataccaaaac tgaagagctc cgagggatat ggataaactc attacacttg
      241 aacaggctgt tggattggtg cgctcaggcg atatgctagc cttaggaggc ataacactct
      301 atcgccgtcc aatagccttc gtacgcgctc ttatccgtcg tttcttcggc gaggcagaac
      361 cgggagacct aaccctgctg gcctttactg ccggcctgga aagcgacctc ctggtgggat
      421 cgggcatggt ctcgcgtata cgcacgtgtt actttggact ggagatcttc ggcctggcgc
      481 caatgttcac ttactttgcc aatcgcggtg aaattgagat catggaggag agtgaggcca
      541 gcctggcatt tgggctgcgg gcgcaaatgg ctggcgtggg tttcatgccc gggctcggct
      601 ggttaggaac cgacttgcct aagcttcgac ctgatgtgct cacagtgacc gacccttaca
      661 atggcgagga gcttatcgcc ttccccgcca tccatcccga tgtggcagtt attcatgctt
      721 tgcaagcaga cccgcatggt aatgcccaga ttggggataa taagggcatc gacgaagagt
      781 tggccctgac tgcccgtacc gtgatcgtca ctgcagaaga gatcgtacca gaaatggaga
      841 aagccaatct ggtagccccc ttggtgcatt acatcgtgca cgcctcacat ggcgcactcc
      901 ctacttcttg tcacccgctt tacccgctgg atggacaagc cttgctggct tatgtcgaca
      961 aggtcagcga acctgaatct tgccgcgcct ttctagaaag ttggatagct gaatagtctc
     1021 actataggtg aatgcagaag cgagggtagt gtggtaattc accccacatt ctagacttga
     1081 taatattttt tataatcctc taatgctatt atggtaatct ttacgatatg aaaaagtatc
     1141 cttattgctc ggtgagtgca gtgattgcat aagagaagcg tcttcaaatc accccaactc
     1201 accaatttac gggatgagtt ttagaagaga tagtggagat ttctcatgaa atccaaggct
     1261 ttagtaacct ttatcagtgt gatcatcgca gggatattgc tgacaggcgc ctgctcagcg
     1321 gggtttgtcg ctgggcgagt attcaatccc cctgagcaga ttttagccct tttgcctggc
     1381 caggaaatca gccccccagt tgctcaagaa gagagcggca cgccacagga gctcgaggag
     1441 ctcttcgtac ccttctggca agcctgggat ctggttcacg accagtatgt agaacagccc
     1501 gtcgatgacg aagtattaat gcgcggcgca attgagggga tgctggattc cctgggcgat
     1561 ccgcacactt cttatctaaa tccagaacag taccagtctc tcaataccca acttcagggg
     1621 gatgaatcct atgaaggcat cggcgcgtgg gtagacacca ccagcgattt cctaaccatt
     1681 atcagtccca tccccggttc cccagcggag aaagcaggtt tgcgcaccgg ggataaggtc
     1741 atcgccgtgg atggtgaaga tatgaccggt atcgatggcg aactagtgcg ccagcgtgtg
     1801 attggtccag cgggcagcac ggttaacctg accatcttgc gcaccggtca agaccctttc
     1861 gatgtacaga tcacacgcgc agagatcact atcccaagtg tggattaccg catgctggag
     1921 gacgatatcg cctatgtgcg cgtcctcatc tttgccgata acactaaaga tcaattacaa
     1981 gatgcgctca aagatctgat gtcccagaat ccacagggca tgattctaga cttacggggc
     2041 aatggcggtg gctatctgga ttctgccatt gatgtcgcct cggagttcat ttccgagggg
     2101 gttatcgcct acgaggagta cggagatggc acgcgggata cctatgatgc ccgacgggga
     2161 ggcctggcta cggacatccc catggtggtg ctaatcaatg agggaagcgc ctcagcctca
     2221 gagattgtcg ccggtgcgat ccaagacctg ggtcgagggc agctcatcgg catgacctcc
     2281 ttcggcaaag gatcggtgca gatccccacg gctctgcgca accaacaggg agcggttcga
     2341 atcactgtag cccgctggct aacacccaaa gagcgcacaa tccacggaaa aggtttaaca
     2401 cccgatgtag aagtgcccgt caccgaagaa gacttcgcca ataatcgcga cccgcagttg
     2461 gagaaagccg tagaaataat tagcaaatga ttgacttttg tcaattaaat caatacacaa
     2521 ctaatgatat gtgagtattt caggctatat caagcctgta tctggtaagg aggtatactg
     2581 tgttcttctt ttatgactac cgttatttga tgttcatgct gccagcattt ctgctgatgc
     2641 tggcagtaca gtggtatgta aattcagcct acaataagtg gggcagggta cctgcccgca
     2701 gccgtatgag cggtgcccag gcagcccagc gcctgatcga gcgcgggggc ttatatgatg
     2761 taaaaatcga gggcgtacaa ggcaac
//
LOCUS       DBNHOH010000491         4550 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_734294, whole genome shotgun sequence.
ACCESSION   DBNHOH010000491 DBNHOH010000000
VERSION     DBNHOH010000491.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_734294"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..170
                     /locus_tag="AC16V7_08570"
     tRNA            87..170
                     /locus_tag="AC16V7_08570"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Leu,seq:cag)
     gene            306..677
                     /locus_tag="AC16V7_08575"
     CDS             306..677
                     /locus_tag="AC16V7_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257215.1"
                     /translation="MIIIGFVVLAILVMDFNNRMSALRRLSTRKEGVLAQLEGQIQTQ
                     DSLQTQIAYATSDASVIEWAYEEGSMVRPGDVPVVPLAQVAGTPVPTPTPVVVSTPIA
                     NWQFWLWLFVDANEPASARAP"
     gene            730..803
                     /locus_tag="AC16V7_08580"
     tRNA            730..803
                     /locus_tag="AC16V7_08580"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:764..766,aa:Val,seq:gac)
     gene            1091..2887
                     /gene="thrS"
                     /locus_tag="AC16V7_08585"
     CDS             1091..2887
                     /gene="thrS"
                     /locus_tag="AC16V7_08585"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR6257216.1"
                     /translation="MAEKKQERYEDSDLYCIRHSAAHVMAQAVVEKFPEAKYTIGPPV
                     EDGFYYDFDLPRSLTPDDLQEIEQRMREIIAGDHPFVKKVVSADEARQIFEQQPYKLE
                     LIEGLEQGGFDEYGQPLAEKPEISIYTHDTFVDLCRGPHVENTRKINPVAVKLMSVAG
                     AYWRGDEHNRMLQRVYGTAWRDAQELEEYLWRIEEAKKRDHRKLGKELGLFYFSDDVG
                     PGLPLFTPKGETLRYLMENFVRDVQTRFGYQHVWTGHLVKEDLYKRSGHYDNYKESMV
                     PPMQDENVVFRLKPMNCPSHMTLFREMGRHSYRELPLRFSEFATLYRYEKTGELTGLT
                     RVRALTQDDCHIFCTEEQVGSEFSLALNLIQEVLARYKFTDYVVRLSLRGEGGKYVQD
                     EEKWVKAEAALREALDASGVEYFEATGEAAFYGPKADFLARDVLGREWQLSTIQVDFI
                     QPARLGLSYIGEDGQEHTPVVLHRAVTGSTERFLGVVIEHFAGAFPVWLAPVQAVLIP
                     IADRHLSYAHQVAEKLKAAGVRVEVDERSERMNAKIRDAQNQKIPYMLVVGDREMEQG
                     AVALRLRSGENPGAISLEEFLERANEDLREGS"
     gene            3063..3533
                     /gene="infC"
                     /locus_tag="AC16V7_08590"
     CDS             3063..3533
                     /gene="infC"
                     /locus_tag="AC16V7_08590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIR6257217.1"
                     /translation="MGPEGENIGVVSIDEALRIARDADLDLVEVAPAADPPVCRVMDF
                     GKFIYERAKKEREARKAQTKIEIKEIRLRPKTNEHHRDLKVRDARRWLEAGMKVKVRI
                     RFRGREISYPEIALSDLKEIAEDLSDLAQIEQAPALEGRTMLMVLAPGKGAKKK"
     gene            3648..3875
                     /locus_tag="AC16V7_08595"
     CDS             3648..3875
                     /locus_tag="AC16V7_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large ribosomal subunit protein bL35"
                     /protein_id="HIR6257218.1"
                     /translation="MPRKQTTGKYKIKTHKATSKRFRLTGSGMLVRTKGGKGHLRRRT
                     SKRTKRLFTEMIPVKGRGIKKRVRRLAPYQK"
     gene            3995..4345
                     /gene="rplT"
                     /locus_tag="AC16V7_08600"
     CDS             3995..4345
                     /gene="rplT"
                     /locus_tag="AC16V7_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIR6257219.1"
                     /translation="MARVKTGPYRRKHHKKILKITKGQFGTRSRLFRRANEAMLKSFW
                     YAYRDRRTRRRELRRLWIARINAAARMNGSTYSRLIHDMKVANVAINRKMLSDLAVRD
                     PNAFAAVVSKVQPG"
BASE COUNT         1115 a         1090 c         1300 g         1045 t
ORIGIN      
        1 ccagacaacc ctctacagca ctggttttca atcgctcagg ttgacaaaac catcctttga
       61 gataaaattc agtccgcagt aaacaagccg aagtggcgga attggcagac gcgctacgtt
      121 cagggcgtag tgagcatacg ctcatgtggg ttcaagtccc accttcggca cacaccttgc
      181 aacatctccc ttgctatgat tagcagggga ggttgtctta acttgaaccg aagggtcttg
      241 cgccaaaggg caggctgcgg taagatgaga ttatgaaggt cccgaatttt agctggagat
      301 acgccttgat catcatcggg ttcgtggtgc tcgctattct ggtgatggat ttcaataatc
      361 gtatgtcagc cttgcggcgc ctgagcaccc gaaaagaggg ggtattggcg cagctggagg
      421 ggcagattca aacccaggat agcttgcaga cccagatcgc ctatgccacc tctgacgcat
      481 ctgtaatcga gtgggcttat gaagagggga gtatggtgcg cccgggggat gtcccggtgg
      541 ttcctttagc ccaggtggct ggcacgcccg ttcctacacc aaccccggtg gtggtttcga
      601 cgccaatcgc gaactggcag ttctggttgt ggctgtttgt tgacgccaat gaaccggcca
      661 gtgcacgcgc cccttgatcc attcttgcct ttttccaatc tgccagttat aatttgcgca
      721 ccattctatg ggcggttagc tcagctggtt agagcgttgc gttgacatcg caaaggtcgt
      781 aggttcgagt cctataccgc ccacaaccgc gatgaccggg tcgagtaccc tggcatctcc
      841 ccgatagaga atggaggcca tcggctgaaa gcctcctcgg cggcgaccag gagaaaaccc
      901 cccgcgagcg tgagccccaa aagcgtgagc tgagtaaggt aaacggttga ctccgttatc
      961 ggttcaaaga gattggcagg actgttcctt aaaggacagt tgggctaaaa tctgggtgga
     1021 accgcgtgaa tagcacctct cgccccagaa gggcgaggtt ttttgttaag ggaaagaaaa
     1081 ggagattaat atggctgaaa agaaacagga gcgctacgaa gatagcgacc tttattgcat
     1141 acgccattcg gcagcacatg tcatggccca agctgtggtg gagaaattcc ccgaggcaaa
     1201 atatacgatt ggccctccgg tggaagatgg cttttattat gatttcgatc tgccccgctc
     1261 cctgaccccc gatgatttgc aggaaatcga acaacgcatg cgagagatca tcgctggcga
     1321 tcatcctttt gttaaaaagg tggtgagtgc tgatgaagcg cgccagatat tcgaacagca
     1381 accctataag cttgagctaa tcgaagggct ggagcagggc ggtttcgatg agtatgggca
     1441 gccactggcc gagaagccgg agatttcaat ttatacacat gataccttcg tagacctctg
     1501 ccgcggtccc catgtagaaa acactcgcaa gatcaatcca gttgcggtca agctgatgag
     1561 cgtggccgga gcttactggc ggggtgacga gcacaatcgt atgttgcagc gagtttacgg
     1621 tacagcatgg cgcgacgcac aggaattgga agaatacctc tggcgtattg aagaggcaaa
     1681 aaagcgcgat caccgcaaat tgggcaaaga gttgggtttg ttttattttt ctgacgatgt
     1741 cggaccggga ttgccgctct ttacgccgaa gggtgagacc ctgcgctacc tgatggaaaa
     1801 cttcgtgcgc gatgtgcaga cgcgctttgg ttaccagcat gtatggaccg ggcacctggt
     1861 gaaagaagat ctatataaac gttccgggca ttacgataac tacaaagagt cgatggtccc
     1921 gcccatgcaa gatgagaacg tggtgtttcg cctcaagccg atgaactgtc ccagccacat
     1981 gacgctattc cgtgaaatgg gaaggcattc ctaccgcgag ctgccgctgc gcttttcgga
     2041 atttgccaca ctctatcgtt acgagaagac cggggagctg actgggttga cgcgtgtgcg
     2101 cgcccttacg caggatgact gccatatctt ttgtaccgag gaacaggtcg gcagtgaatt
     2161 ttctctggct ctaaatctga tccaggaagt gctggcccgc tataaattta ccgattatgt
     2221 ggttcgattg tcgctgcgcg gcgagggcgg gaaatatgtc caagatgagg aaaagtgggt
     2281 taaagccgag gcggctttgc gtgaggccct ggatgccagc ggtgtggagt actttgaagc
     2341 caccggcgaa gcggctttct atggtccaaa agccgacttc ctggcgcggg atgtattggg
     2401 ccgcgaatgg cagctatcta cgatccaggt ggatttcatc cagccagcgc ggctggggtt
     2461 aagttatatc ggagaagacg gtcaggagca cacaccggtc gtgttgcacc gcgccgttac
     2521 aggctccacg gaacgctttt tgggtgtcgt catcgagcac tttgcagggg cattcccggt
     2581 ttggctggca ccagtacagg ctgttcttat cccgattgct gaccgtcact tatcatatgc
     2641 tcaccaggtt gccgagaaac tgaaagctgc gggtgttcgt gtcgaagtgg acgagcgcag
     2701 cgaacgtatg aacgccaaaa tacgtgatgc gcagaatcaa aaaatcccct acatgcttgt
     2761 ggtaggcgac cgggagatgg agcaaggtgc ggttgccttg cgcttgcgct cgggcgaaaa
     2821 cccgggagcg atatcactgg aagaattttt agagcgagcg aacgaggatt tgagagaagg
     2881 aagttagatt ttatctccct ggatatactc gaaattctgt tgacgatttt cgccgattca
     2941 tggtatcatg agtcacactg gctttgcgcc agtgatttgt ttcagcattt gagaaggagt
     3001 gacatatcag cgcagcgaat taccgtgtaa atgaatccat ccgtgtccct gaggtacgcc
     3061 tgattgggcc cgaaggtgag aatatcggtg tagtatcaat cgatgaagct ctaaggatag
     3121 cgcgtgatgc tgatttagac ctggtggaag ttgccccagc agccgatcct cctgtttgca
     3181 gggtgatgga ttttggaaag ttcatctatg aaagagcaaa gaaggaacgg gaggcgcgaa
     3241 aagcccagac gaagattgag attaaggaga ttcgcctaag gccaaagacg aacgagcacc
     3301 accgcgacct gaaggtccgt gatgcacggc gctggttgga agccggtatg aaagtaaaag
     3361 ttcgcattcg cttccgcggc cgggaaatca gttatcctga aattgctctc tccgacctga
     3421 aagaaatcgc tgaagatctt tcagatttgg cgcagattga acaagcccca gcattagaag
     3481 ggcgtaccat gttgatggtg ctcgctccgg gcaagggtgc aaagaagaag taattccgct
     3541 gagttgtagt ccatataaat aatgttgagt cagacgatcc gcggatcgta gagatggcgg
     3601 tcatcccgcc cgaaaaccgc ggaatttttt gtgagaggag ttcggacgtg cctcgcaagc
     3661 aaacgacagg aaaatataaa atcaaaaccc acaaggcgac ctccaaacgc tttcgcctga
     3721 ctgggtcggg aatgttggtg cgaaccaaag gcggtaaggg tcacctgcgc cgtcgaactt
     3781 caaagcgcac caaacgcctg ttcaccgaaa tgatccccgt gaaaggtcgt gggattaaaa
     3841 aacgcgtgcg tcgccttgcc ccgtatcaga agtaaattgt attgggtcag gttgaggctc
     3901 gcagaaattt catcttttga ttgcggctac tgggtgtaag caacaggtgc tcttatgaag
     3961 taccggcgcc caggggttcg caggagagta tagaatggct cgtgtaaaga caggtccgta
     4021 ccgtcgcaag catcataaaa aaatcttaaa aatcaccaaa ggtcaattcg gcacgcgctc
     4081 gcgccttttt cgccgtgcta atgaggccat gctgaagagc ttttggtacg cgtatcgcga
     4141 tcggcgtacc cgcaggcgcg aactgcgcag gctgtggatt gcgcgcatca acgccgcggc
     4201 acgcatgaat ggctccactt acagtcgatt gatccacgac atgaaggtcg ccaatgtagc
     4261 gatcaaccgc aagatgcttt cagatctggc ggttcgcgac cccaatgcct tcgctgcggt
     4321 cgtgagtaaa gtccagcccg gataataaaa ttgatcaatt ctcccagccc ttacctcaat
     4381 tcgctggagg agggtgagtt tgcggatgtt tcggcggaat tctccgctga aacatccgca
     4441 atattcgtta cctatctggt tcggggttga ataggggctg atgaagtatt ttgtgtaagg
     4501 caagtgtttt gaggtcctat gtccagtaat cgcaatcgct caatcacccc
//
LOCUS       DBNHOH010000492         2262 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1440138, whole genome shotgun sequence.
ACCESSION   DBNHOH010000492 DBNHOH010000000
VERSION     DBNHOH010000492.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1440138"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="AC16V7_08605"
     CDS             <1..198
                     /locus_tag="AC16V7_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257220.1"
                     /translation="DSPPLRRENGVFLVGAYPHQNTPNTLFAGLRMPFRAYAIALAFD
                     KYSCNPALPVLEYDTIQAREK"
     gene            202..855
                     /locus_tag="AC16V7_08610"
     CDS             202..855
                     /locus_tag="AC16V7_08610"
                     /inference="COORDINATES: protein motif:HMM:NF014618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HIR6257221.1"
                     /translation="MSDMVEVVIDSIRVSLMSQQRIVILREVNADRYLPIWIGVYETE
                     AITIALQDVEVARPLTHDLLKNIFSALNAQIIRVEVSALKDDTFFGNIIAEVNGRNLN
                     VDARPSDALNLAVRAHVPILVARSVMDAAGITPEEDIQEQKQIESKTQGETPEPSEES
                     QERLSVFEDFLEKLELEDEDDENGKEDDNGGKGKKDDDSGKGKEDDDGGKGKKDKEE"
     gene            1024..>2262
                     /gene="dnaB"
                     /locus_tag="AC16V7_08615"
     CDS             1024..>2262
                     /gene="dnaB"
                     /locus_tag="AC16V7_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR6257222.1"
                     /translation="MNDRFFPEENPTPASPPVLYSREAEEAVIGSVLINPEAYYDVAS
                     FLKADDFHIHRHRWIWEVFVRLHERRIPIDYLTVSEELNQLGQLSEVGGPAYLTALIN
                     NVPTSLHAEAYGRLVEENAIRRKMLAAANDIAKLAYQESIGVEDVMDGAEKSIFGVSE
                     RRMTRDLQPIQQVLSEYYDRIDQLASRGEDIYGVPTGFIDLDRLLMGLQPSDFIIVAG
                     RPGMGKTAFMLSAAKNAAQTHKKHVAVFSLEMSNEQLVQRLIAQETGIDSQRLRTGKL
                     SEDEWPLFTHAIEVLSDARVFLDDTPALTPLQLRTKSRRLHLEYGLDLIVVDYLQLMS
                     SGTRSENRVQEVSYISRNLKVLARELNVPVLAAAQLSRAVEQRADKEPQLSDLRESGS
                     LEQDADIVMFIHRPEVYEKDT"
BASE COUNT          556 a          610 c          624 g          472 t
ORIGIN      
        1 gattccccac cactgaggag agaaaatggg gtgtttttgg tgggggcata cccccaccaa
       61 aacaccccaa acactctttt tgcgggattg cgcatgccct tccgggcata tgcaattgcc
      121 ctggcctttg ataaatatag ttgcaaccct gccctgcccg tgttagaata cgatacaatc
      181 caagctaggg aaaagtgatt catgtcagat atggtcgaag tggtaattga tagcatccgg
      241 gttagcctga tgtcgcagca acgcattgta attctgcgcg aggtcaacgc ggaccgctat
      301 ttgcccattt ggatcggagt ttacgagaca gaggcgatca ccatcgccct gcaagatgtc
      361 gaggttgcac gaccattaac ccatgactta ttaaaaaata tcttcagtgc cctaaacgct
      421 cagatcatcc gggtcgaggt gtctgctttg aaagatgaca ccttctttgg caacatcatc
      481 gctgaggtga acgggcgcaa tctgaatgta gacgcccgcc cttccgatgc gctgaacctg
      541 gcagtgcgtg cccatgtccc catcctggtc gcccgctccg tgatggatgc cgctggcatc
      601 acacctgagg aagacatcca ggagcagaaa caaattgaaa gcaagacgca aggcgagacc
      661 ccagaaccat ctgaggaaag ccaggaacgt ttgtcggtat ttgaggactt cctcgaaaaa
      721 cttgaactgg aagacgagga tgacgaaaat gggaaagagg acgacaatgg tggcaagggg
      781 aaaaaggacg atgatagtgg caaggggaaa gaggacgatg atggtggcaa ggggaaaaag
      841 gataaggagg agtagagcgc tgcgtactcc cgattgacaa atgtgcaccg gggttcgcca
      901 ggcagccaaa ctggtgtccc cggttttttg gtcaaaaccg gaccaccctc tcagattgcc
      961 ggatttgcag gaacagcgca gcgctttttg cctaatctga tatgcaggta tagataggat
     1021 gtgatgaacg atagattctt tcccgaagaa aacccgaccc ctgcttcccc acctgttctc
     1081 tatagccggg aagccgaaga ggccgtcata ggatccgtgc tgattaaccc cgaggcgtat
     1141 tacgatgtcg cctctttcct gaaagccgat gattttcaca tccaccgtca ccgctggatc
     1201 tgggaggttt tcgtacgcct gcatgagcgg cgcatcccaa ttgattatct aaccgtttcc
     1261 gaagaactca accaactggg tcaattatcc gaagtgggcg gtccagctta cttgacggca
     1321 ctcatcaata acgtgcccac ctcgctccat gcggaggcgt atggacgcct ggtggaagaa
     1381 aacgcaattc gccgcaaaat gttagccgcg gccaatgaca tcgccaagct cgcctaccag
     1441 gagagcatcg gcgtcgaaga tgtgatggac ggagctgaga aatccatctt tggcgtgagt
     1501 gagcgccgca tgacgcgcga tctgcagccc atccagcagg tgttgagcga gtattacgac
     1561 cggattgacc agcttgccag ccgtggcgag gacatctacg gcgtgccgac aggattcatc
     1621 gatctggatc gcctgttgat gggcttgcaa ccctcggatt tcatcattgt cgccggacgc
     1681 ccaggcatgg gcaagacggc ttttatgctc tcggctgcca aaaatgccgc ccagacccac
     1741 aagaagcatg tggcagtgtt ctcgctagag atgtccaacg agcaactcgt gcagcgcctg
     1801 atcgcccagg agaccggcat tgactcgcag cgcctgcgca ccggcaagct ctctgaggat
     1861 gagtggccgc tgttcacaca cgccatcgag gtgttgagcg atgcgcgcgt cttcctggat
     1921 gatacgcccg cactcacccc cttgcaatta cgcaccaaga gccgccgctt gcacctggag
     1981 tatgggctgg acctgatcgt agtagattac ctgcaattaa tgtcgagcgg cacgcgctcc
     2041 gagaaccgcg tccaagaggt ttcttacatc tcgcgcaatc taaaagtgct tgcgcgcgag
     2101 ctcaatgtac ctgtgctggc tgctgcgcag ctatcacgcg ccgtggagca acgcgcagac
     2161 aaggagccgc aactctccga cctgcgcgaa tcgggatcac tggaacaaga cgcggatatc
     2221 gttatgttta ttcaccggcc cgaggtgtac gagaaagata cc
//
LOCUS       DBNHOH010000493         2919 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_156861, whole genome shotgun sequence.
ACCESSION   DBNHOH010000493 DBNHOH010000000
VERSION     DBNHOH010000493.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_156861"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="AC16V7_08620"
     CDS             <1..252
                     /locus_tag="AC16V7_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257223.1"
                     /translation="RFQPEGLRLGRQAVGVSPRFLAIGKFRSSKVRIFLWVYWWGGQA
                     RAGMTPVTHWPKILPGLRLERRGLGIQEYLVSKLSEYKL"
     gene            complement(301..1572)
                     /locus_tag="AC16V7_08625"
     CDS             complement(301..1572)
                     /locus_tag="AC16V7_08625"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HIR6257224.1"
                     /translation="MQPIHHPVELSLDQCIKCNICTTVCPVSAVTDLFPGPKYEAPQA
                     GRFRAPGQQTPDHSVDYCSGCRVCNMACPTGVKIAEMNARARATLVEQGKISPILRLR
                     NNLIARAELLGKIATPIAPLANAILSNRAIRWTMEQALGIHREAPLPRFSGERFTTWF
                     NAHQSTNRAWRKVVYFHGCSTQYYEPRVGRAAIGVLEANGYEVIVPPQNCCGLPLLSN
                     GEFPAARNYHTSNVNHLVGYAKQGIPIVGTSTSCTLTLKEEAPELLDMVDEDTRLVAQ
                     STYDINEFLLDRFAEGKFRIDFQPIPLTLAYHAPCQYRAHRLGKLALEILALVPELNI
                     LDSQVACCGIAGTYGYKREKFDVAMQVGEPLFNFVRDVGGPAVICDSETCRWQITQAT
                     GLPAIHPIEILAAAYGLKVEGILQDLLASSS"
     gene            complement(1582..>2919)
                     /gene="glpB"
                     /locus_tag="AC16V7_08630"
     CDS             complement(1582..>2919)
                     /gene="glpB"
                     /locus_tag="AC16V7_08630"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR03378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HIR6257225.1"
                     /translation="MFLPEQSQTPTSRTPAPITKREGVRKRSLLDILVIGGGLAGLFS
                     AWKVAQKGKKVRLVTKGWGALHWGSGCINVLGYYPLDSTDPLQSPVAGIKQILRENSK
                     HPYALVQLEHIEAALEDFKTLSAQLGYPFHGDLDRNWLLPSALGTFRPACLVPETMVA
                     GDLRQNQPMLIVGFEQYPDFYSELIAANLTRQGRQALGVTIDLPSLRQQRFVSGRVLA
                     SLFESAEFRREVAWQLKTHLQGAKSVGFPAVLGLKDTLKVKNELETLLEMPVFEIPTL
                     PPSIPGIRLHNLLIAEIERLGGQVYDGMQAQSAAYDGARILTVFTEAAARLKPHHANT
                     FILATGGILGGGIHFEYGQKPSESVFNLPVMAPDDRNNWFESQFLTQLSHPIYQSGIL
                     VSDEFKPIQANGSHLFENLYIAGNQIGSCDSIRERSAEGIALATAYSISNQLS"
BASE COUNT          683 a          679 c          809 g          748 t
ORIGIN      
        1 agatttcagc cagagggtct gcgattgggc cgtcaagcgg taggtgtatc accgcgattt
       61 cttgcgattg ggaagtttcg gtcgtcgaag gtacggattt ttttatgggt gtattggtgg
      121 ggaggacagg ctcgggctgg gatgacacct gtaacgcact ggccgaagat actgcctggc
      181 ttgcggttgg agagacgagg gctgggaatc caggaatacc ttgtgtcgaa gctgagcgag
      241 tacaagctgt gataaataat atgagcagta gctctacttt gtaaataagc tgtttggtgg
      301 tcaagagctt gaggccagta aatcttgtaa tataccttcg acttttagac cgtatgcggc
      361 tgcgagtatt tctatcggat gtattgccgg caagcctgta gcctgagtga tctgccagcg
      421 gcaggtttcg ctatcacaga tgacggctgg tcccccaacg tctctgacga aattaaaaag
      481 tggttctcct acctgcatcg ccacgtcaaa cttctctctc ttgtagccat aggtcccagc
      541 gatgccgcag caggcaacct ggctatccag aatgtttagc tctgggacga gagctaatat
      601 ttccagagcc aactttccga ggcgatgagc gcggtactgg catggagcgt ggtaggctag
      661 tgtgagtgga atgggctgga agtcaatgcg aaattttccc tcagcgaagc gatccagcag
      721 gaattcgttg atatcatatg tggattgtgc gaccaggcga gtatcttcgt caaccatgtc
      781 gagcaattcg ggtgcttctt ctttcaaggt taaggtgcag cttgtggatg tgccgacaat
      841 cggaatgccc tgtttcgcat aacctactag gtgatttaca ttgctggtat gataattacg
      901 ggcagcggga aactcgccat tagaaagtaa tggtagaccg cagcaatttt ggggaggcac
      961 gatgacctcg taaccatttg cttccagcac cccgattgct gcacgtccga cgcgcggctc
     1021 gtaatattgc gtggagcaac catgaaagta gacgactttt cgccacgctc tgttagtgga
     1081 ttggtgtgca ttgaaccagg tagtaaatcg ctctccggag aaacggggta ggggggcttc
     1141 ccggtggata cccaatgcct gttccattgt ccagcggata gcacgatttg ataaaatggc
     1201 attcgccagt ggtgcaattg gggtggctat tttgcctagt agctcggcgc gggcgataag
     1261 attatttcgc aggcgcagta tcggagaaat ttttccctgt tccaccagtg tggcgcgggc
     1321 tcgagcgttc atctcggcta ttttgacacc tgtcggacaa gccatgttac atacacgaca
     1381 gccgctgcag taatctaccg aatgatcagg ggtttgctgt cccggtgcgc ggaaacgccc
     1441 tgcctggggg gcttcatatt ttgggccagg aaacaagtca gttaccgcgg aaaccgggca
     1501 taccgttgtg cagatattgc acttgataca ctgatctaga cttagttcga cagggtgatg
     1561 tattggttgc atggttggct gtcagctcag ttggttggat atgctgtatg cagtggctaa
     1621 ggcgatgcct tcggcggaac gctcgcgtat ggaatcacag gaaccaattt ggttgccagc
     1681 gatgtagaga ttttcaaaga gatggctgcc attggcttga atgggtttga attcatcgct
     1741 caccaatatg cctgattggt aaattggatg gcttaattgg gtaaggaatt gggattcaaa
     1801 ccaattattg cgatcgtctg gtgccataac gggcaaattg aacacacttt cactgggttt
     1861 ttgcccatat tcaaaatgga ttcccccacc caagatacct ccggtagcaa ggatgaaggt
     1921 gttggcatga tggggcttta agcgcgctgc ggcttccgta aaaacggtta atattcgtgc
     1981 tccatcataa gcagccgatt gggcttgcat accatcgtat acctgcccgc cgagccgctc
     2041 aatctctgca atcaacaggt tgtgcaaacg gatcccaggg atggagggtg gcagggtggg
     2101 gatctcaaaa accggcattt cgaggagcgt ttcgagttca tttttgactt taagagtgtc
     2161 tttcaggccc aagacagccg ggaaacccac actttttgcg ccctgcaaat gtgtttttaa
     2221 ttgccaagcg acctcacgac gaaactcggc tgactcaaac agtgatgcta atacccgacc
     2281 gctgacgaag cgctgctggc gcagagacgg taaatcaatt gtcaccccta aagcttgcct
     2341 accttgtcga gtgaggtttg cggcgatcag ctcggaatag aagtcaggat actgctcgaa
     2401 tcccacgatg agcatcggtt ggttctggcg taggtcgccg gccaccatag tctcaggcac
     2461 caggcaagcg ggacggaatg tccccagcgc cgaggggagt agccagtttc tgtccaaatc
     2521 tccgtgaaat ggatagccca actgagcaga caaagtttta aagtcttcca gcgcagcttc
     2581 aatatgctcc agttggacca atgcgtatgg gtgttttgaa ttttcgcgga gaatttgctt
     2641 tatccccgct acaggtgatt gtaaaggatc tgtgctatct agtgggtagt aacctaatac
     2701 gttaatgcac ccagagcccc agtgaagcgc accccagcct tttgtaacca ggcgcacttt
     2761 tttacccttc tgcgctactt tccaggcaga gaatagccca gccaaaccac ctccaatgac
     2821 aaggatatcc agtaatgatc tctttctcac cccttcacgc tttgtaatcg gcgcaggagt
     2881 tcgggaggtt ggggtttgag attgttccgg taggaacat
//
LOCUS       DBNHOH010000494         1114 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1247070, whole genome shotgun sequence.
ACCESSION   DBNHOH010000494 DBNHOH010000000
VERSION     DBNHOH010000494.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1247070"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="AC16V7_08635"
     CDS             complement(<1..251)
                     /locus_tag="AC16V7_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257226.1"
                     /translation="MLIVSVGMPRAGSGWYYNLTHDMVLAAGGQDARRIRQRYYLGRI
                     LTEVNCNIGAFTPPRLLAVLLPAMLGNTFVVKAHAAPTPL"
     gene            435..>1114
                     /locus_tag="AC16V7_08640"
     CDS             435..>1114
                     /locus_tag="AC16V7_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HIR6257227.1"
                     /translation="MDRYRVIPESRVELSDYDPGDTAEFSGDKDEGKDRLDELNDQLE
                     ALQELLYAEHKHKLLIVLQGMDTSGKDGVIRHVFDGVNPQGVRVANFKVPTPQELDHD
                     YLWRVHQQVPGRGEIVIFNRSHYEDVLVVRVHELVPKERWSRRYQQINDFERLLVEEG
                     TTILKFFLHIDQQEQKERLQARLDDPNKRWKFNVGDLKERKLWNEYRWAYEDVLSQTS
                     LEVAPWYIV"
BASE COUNT          274 a          248 c          350 g          242 t
ORIGIN      
        1 agcggggtgg gggcggcatg ggctttgacg acgaaggtgt tgcccagcat ggctggcagg
       61 agcactgcca gcaggcgcgg cggtgtaaac gcgccaatat tgcaattgac ctcggtaagg
      121 atgcgaccaa ggtagtaacg ctggcggatg cgacgagcgt cctgcccgcc tgcggcgaga
      181 accatatcat gagtcaggtt atagtaccag ccggagccgg cacgcggcat gcctacagag
      241 acgatcagca tgagtttgat tatagaactt ttacagttga atgaaaagat aggcgaatcg
      301 gggagcttcg atggatgttt tgagtgggtc agtgctatgg ttttataata ggataccttg
      361 gctagacagg taaggttttt aggaatagac ttacagcttg aaagatttac atcaagaagg
      421 aggagacttg ctctatggac cgttaccgag ttattcccga gtcacgggtt gagttgagcg
      481 actacgaccc aggcgatacc gccgagtttt cgggcgataa agatgagggt aaagacaggc
      541 tggatgagct taacgaccag ctggaggcgc tgcaggaatt gctctacgcc gagcacaagc
      601 ataagctcct gatcgtattg caggggatgg atactagcgg gaaggatggc gttatccgcc
      661 atgtattcga cggcgtgaac ccccagggcg tgcgcgtggc aaatttcaag gttcctaccc
      721 cgcaggagct cgaccatgat tatctatggc gcgtccatca gcaagtgcca gggagagggg
      781 agatcgtcat cttcaaccgc tcgcattacg aggacgtgct ggttgtcagg gtgcacgaac
      841 tggtgccaaa ggaacgctgg tcgcgccgct accagcaaat taacgatttt gagcgcctgc
      901 tggttgaaga ggggacgacc atcctgaagt tctttctgca cattgatcag caggaacaga
      961 aagagcgcct gcaagcgcgc ctggatgacc cgaataagcg ctggaagttc aatgtgggcg
     1021 atctcaagga gcgcaagttg tggaatgaat atcggtgggc ttacgaggat gttctcagcc
     1081 agaccagtct tgaagttgct ccttggtata ttgt
//
LOCUS       DBNHOH010000495         1808 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_221378, whole genome shotgun sequence.
ACCESSION   DBNHOH010000495 DBNHOH010000000
VERSION     DBNHOH010000495.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_221378"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="AC16V7_08645"
     CDS             <1..446
                     /locus_tag="AC16V7_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HIR6257228.1"
                     /translation="INQVLDERAHFGRVIILYGARAPAELLFKDELRQWEARGDVELH
                     LTVDRGDEHWKGHIGVITTLFKDISVYPRNTVAVTIGPPVMYRFVLMELLGTGISEGN
                     IWLSLERRMKCGIGKCGHCQINNVYTCQSGPSFSYKEIKGLEEAL"
     gene            443..1219
                     /locus_tag="AC16V7_08650"
     CDS             443..1219
                     /locus_tag="AC16V7_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HIR6257229.1"
                     /translation="MSQSNRPKVAFFDFTCCEGCQLTVLDSLQDHPDLLGAVEIVQFR
                     EAMSERSDDYLIAFIEGSCSRPADEERLKHIRERASIVVALGACAHLGGVNALKYFIP
                     LDDARSYVYGDKAGWYDTGDVRPVGAVIPVDVFIPGCPIDRGEFIACVKALLLGKKPP
                     VPDYPLCVECKLQENACLYTRGNICMGPVTRAGCGAICPTYGQSCESCRGFVSNPNDS
                     SMRELLAKHGLSMDEVNMQYTLYNTYSMRDELKRRQSVHG"
     gene            1212..>1808
                     /locus_tag="AC16V7_08655"
     CDS             1212..>1808
                     /locus_tag="AC16V7_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HIR6257230.1"
                     /translation="MGRRIDVHYVTRVEGHGNIVVEMGNGKLKDCRFEVVEAPRFFES
                     MLRGRHYSEAAQITSRICGICSTGHTTTSLRATEKALGVVPSEQTRLLRKLAFYGEML
                     DSHVLHAYMLVAPDFLGVGSVIPLASTHPEVVLRALRMKKLAGDLCAAINGRHTHPIA
                     MTVGGFTHFPNPDELEDLRYRLEEARRDVDATVDLFASL"
BASE COUNT          390 a          432 c          545 g          441 t
ORIGIN      
        1 tgatcaacca ggtgcttgat gagcgtgctc atttcggccg ggtcatcatc ctgtacgggg
       61 cgcgcgcccc agcggagctg ctcttcaagg atgagctacg ccagtgggag gcgcggggag
      121 atgtggagtt acacctgaca gttgaccgtg gcgacgagca ttggaaaggg catatcggtg
      181 tgatcaccac gctgttcaaa gacatctctg tctacccacg taacacggtg gcggtaacca
      241 ttggcccccc ggtgatgtat cgctttgtgc tgatggagct gctgggtact ggcatctcag
      301 agggaaatat atggctatcc cttgagaggc gaatgaagtg tggcataggg aagtgcggac
      361 actgccagat caataatgtc tatacttgcc aatcaggccc ctcctttagc tacaaggaga
      421 tcaaaggtct ggaggaggca ttatgagcca gagtaaccgg ccgaaggtgg ctttttttga
      481 cttcacatgc tgcgaaggct gccagctcac cgtattggat tcattgcaag accacccaga
      541 tttgctgggt gctgtagaga tcgtccagtt tcgcgaggcg atgagcgagc ggagcgatga
      601 ttatttgatc gccttcattg aaggctcttg cagccgtcct gcggatgagg aacgcctcaa
      661 acacatcaga gagcgcgcct ccattgtggt ggcgctcggc gcctgtgccc atctgggtgg
      721 tgtaaatgcc ttgaaatatt ttattccgtt agacgatgcc cgctcatacg tctatggcga
      781 caaggccggc tggtatgaca ctggcgatgt tcgtccggtg ggggcagtca tcccagttga
      841 tgtttttatc cctggttgtc caattgaccg aggggagttc atcgcttgtg taaaggcttt
      901 gctgttaggt aaaaaaccac ctgtcccaga ttacccgttg tgcgttgagt gtaaactgca
      961 agagaatgcc tgtctgtata cgcgtggaaa tatctgcatg ggtcctgtca ctcgggcggg
     1021 ttgcggggct atctgcccca cctatgggca aagttgcgaa tcttgccggg ggtttgtctc
     1081 caatcccaac gattcgtcca tgcgtgagct tcttgccaaa catggcttga gcatggacga
     1141 ggtgaatatg cagtacacgc tctacaacac ttattctatg cgtgatgaac ttaagcggag
     1201 gcagagtgtc catgggtagg cgtatcgatg tccattatgt aacccgcgtg gaggggcacg
     1261 gcaacattgt ggtggagatg ggtaatggca agctgaagga ttgccgtttt gaagtggttg
     1321 aagcaccacg tttcttcgaa tcgatgttgc gcggccggca ttattccgag gctgctcaga
     1381 tcaccagccg tatctgcggg atatgttcaa cagggcacac caccacctca ctccgagcga
     1441 cagaaaaggc tctgggtgtg gtgccgagcg aacagacccg attgctgcga aaattggctt
     1501 tctatggcga gatgctggat agccacgtat tgcacgcgta catgcttgtc gccccagatt
     1561 tcttgggcgt gggtagtgtg atccctttgg cgagcacgca ccccgaggtc gtgctgaggg
     1621 cattgcgcat gaagaagctg gccggagatc tatgcgctgc cattaacggg cgccacaccc
     1681 acccgattgc gatgacagta ggtgggttta cacactttcc aaatccggat gagttggaag
     1741 atttgcgtta tcggctggag gaagctaggc gggatgtgga tgcgacagtg gatttatttg
     1801 catcactg
//
LOCUS       DBNHOH010000496         1789 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_478187, whole genome shotgun sequence.
ACCESSION   DBNHOH010000496 DBNHOH010000000
VERSION     DBNHOH010000496.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_478187"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /locus_tag="AC16V7_08660"
     CDS             complement(<1..1192)
                     /locus_tag="AC16V7_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR6257231.1"
                     /translation="MNPIRIGIDIGGTFTDFVIFRPDTGRIETFKLPSTPHHPAEAVL
                     TGLRRVIGSENNLVIEIIHGSTVATNALLERKGARTALITTRGFGDVIQIGRQDRPSL
                     YDLNFVPAQPLVPEDLRFEVDERVNSSGNVQQALSVEQVKEISSYLKQHNPESIAICL
                     LFSFLYPEHERRIAEQLRQEGYFVSLSCEVLPEFREYERTSTTVVNAYVSPVLDAYLD
                     QLETGLRSISSSKLQIMQSNGGAISPYEARKKGVHCILSGPAGGVIGSQYIANQTEVT
                     NETSTGSEDSLKLITFDMGGTSTDVSLLVGEPNITTEATISGLPIRIPLLDIHTIGAG
                     GGSIAYVDAGGALRVGPQSAGADPGPACYGRGELPTVTDANLVLGRLDPDHFLGGKMP
                     LEAER"
     gene            complement(1271..>1789)
                     /locus_tag="AC16V7_08665"
     CDS             complement(1271..>1789)
                     /locus_tag="AC16V7_08665"
                     /inference="COORDINATES: protein motif:HMM:NF024245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR6257232.1"
                     /translation="SVTQFVAGGAGESDLELLSTTEHLYRQLRLQRTYFMAFRPIPDT
                     PMENQPAVSPLRERRLYQASFLIRDYQFSYEEFLFDQRGNLPVETDPKTAWAEIHLSG
                     RPMEINHASRQELLRIPGVGPKGAGAILAARRLRKFRSLSELNKIGINTHRIAPFIII
                     DGKLPTRQGKFW"
BASE COUNT          475 a          436 c          438 g          440 t
ORIGIN      
        1 atcgctcggc ctctaatggc attttaccgc ccaagaaatg atccgggtcc aaacgaccaa
       61 gaactaagtt agcatcagtc acagttggta actcaccgcg cccataacaa gctggaccag
      121 ggtcagctcc agcgctttga ggaccgacgc gcaatgcacc tcccgcatca acatatgcta
      181 tagatccacc tcccgcgccg attgtatgga tgtccaatag agggatccga atgggcaacc
      241 cgctgattgt ggcttcagtg gtaatgtttg gctctccaac cagcaacgac acatctgtgg
      301 aggtgccacc catatcaaaa gtgatcagtt taagtgaatc ctccgaacca gtactggttt
      361 cgtttgttac ctctgtttga ttggcaatat attgtgaccc aataacaccc ccagcaggcc
      421 cagataagat gcagtgcaca ccttttttcc gagcttcata agggctaatc gcaccgccgt
      481 tggattgcat aatctgcagt tttgaacttg agattgagcg caatcccgtt tccaactgat
      541 cgagataagc atccaagact ggcgatacat aggcgttaac cacggttgtg ctggttcgct
      601 cgtactcccg gaattccggc aatacctcgc aggataatga aacaaaatat ccttcttggc
      661 gtaattgctc ggcaattcgc cgctcatgct caggatatag gaaagaaaag agcaggcaaa
      721 tagcaatcga ttcgggatta tgttgtttta gataggaaga aatttcctta acctgttcaa
      781 cgcttaatgc ttgctggaca ttgccactgc tgttcacacg ctcatctact tcaaaccgca
      841 gatcttccgg cacaagtggt tgggcaggga caaagtttaa atcatataga gaaggacggt
      901 cctggcgacc aatttggatt acatcaccaa agccgcgtgt cgtaataagg gcggtgcgtg
      961 ctcccttgcg ctccagaagt gcgtttgtag caacggtcga gccatgaatt atctcgatga
     1021 ccagattatt ctccgagcca atcacgcgcc ttaaacccgt taacaccgcc tcggcagggt
     1081 ggtgaggcgt agaaggtaat ttaaacgtct cgatgcgtcc agtatcagga cgaaagatga
     1141 caaaatcggt gaatgtcccg ccaatgtcga tgccgatgcg gatcgggttc attagccaac
     1201 ctttggaaaa gtatagaaca agctagagaa atctgcaaga gagcaagcgt gaggtcagaa
     1261 gatgggattt ttaccaaaat tttccctggc gcgttggtaa tttgccatct atgataataa
     1321 agggagctat ccgatgcgta ttaataccaa tcttatttaa ttcgcttagt gatcgaaatt
     1381 tacgcaggcg gcgggctgcg agaatagcgc cagcgccttt tggacctacc ccgggtatcc
     1441 gcagaagttc ttggcggcta gcatggttta tctccatggg tcgcccagat aaatgtattt
     1501 ctgcccaggc agttttagga tcagtttcca cgggaagatt tccgcgttga tcaaacagaa
     1561 attcctcata actgaactga taatcgcgaa tcaggaaaga tgcttggtaa agacgtctct
     1621 cccgcagtgg cgaaactgcc ggctggtttt ccattggggt gtccgggata ggtcggaaag
     1681 ccatgaaata tgtcctctgc aaacggagct gtcggtatag atgttcagtg gtggacagta
     1741 attccagatc ggactcacca gcacccccag cgacgaactg tgttaccga
//
LOCUS       DBNHOH010000497         1579 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_927682, whole genome shotgun sequence.
ACCESSION   DBNHOH010000497 DBNHOH010000000
VERSION     DBNHOH010000497.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_927682"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="AC16V7_08670"
     CDS             complement(<1..707)
                     /locus_tag="AC16V7_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257233.1"
                     /translation="MNRHSFMGEIERIFRIALIQKIIFMIMRAAWVGGAVYLACWGTN
                     RIWGWFSNERSWSIIALITSVIIFCTVFLVTKPSRKFVWRLDREYSLQEQVFTLYELL
                     QEGEPGVENQPGVRELIDSEHISRLAEVRRKLVDKGWRVKEEFEATIVVLILLMIVYL
                     TSVSSIGGIPPGASIGILPSIGIDPSIEQVFPRGIPGDLGNGGLLEDGSAISINEDGI
                     SMPEFTKMEWVQIRAMMR"
     gene            complement(704..1474)
                     /locus_tag="AC16V7_08675"
     CDS             complement(704..1474)
                     /locus_tag="AC16V7_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257234.1"
                     /translation="MFGWDAFVEFLQAIAENQGYRQALIDVYGVNLQDLQTHWSEYFP
                     VYINVRWQANVIHSYDLSQFGQLITQGAYEDAAKGLESAIPLIELFGNPEQLSEAKSL
                     FAKAQVGKEAARLALDARQAILDLDYVRGHRSAEQALMLYRQLGDTRRIAEIETYRDI
                     CAEVLTLRAELDSLRGVGTPLDPLRTQRIVTIGQRLAQLGDSEGANQVQIALLLLGTG
                     QQNFVNWVTVVGLVICIYLIWRRVAYVRKHLSSKVNLI"
BASE COUNT          435 a          445 c          302 g          397 t
ORIGIN      
        1 cgcatcatcg ctcttatctg gacccattcc attttggtaa attccggcat tgaaatcccg
       61 tcttcgttta tagaaatcgc actaccatct tccaatagcc caccattgcc taaatccccc
      121 ggaattccgc gaggaaatac ttgctctatc gaaggatcga tcccaattga gggcaagatt
      181 cctattgatg ctcctggggg aattcctccg atactgctaa cacttgtcag gtatacgatc
      241 atcagcaaga tcaaaaccac tatggtagct tcgaactctt cttttaccct ccagccttta
      301 tccaccagtt ttcgacgaac ttctgccaaa cggcttatat gttcgctgtc gatcaattcc
      361 cggacaccag gttggttctc aacccctggc tcaccttcct ggagcaactc ataaagagta
      421 aaaacttgct cctgaagaga atactctcta tccaatctcc aaacaaattt ccgcgagggt
      481 ttcgttacca aaaatacagt gcaaaaaatg atgacagaag tgatcagtgc aattatgctc
      541 caggaccgtt cgtttgaaaa ccaaccccaa attcgatttg tcccccaaca tgccaggtag
      601 actgcccccc ctacccaggc tgcacgcatg atcatgaata taattttttg aatcagggcg
      661 atcctgaaaa tcctttcaat ctcacccatg aaagaatgcc gattcatatc agattcacct
      721 ttgaggatag atgtttccga acgtatgcaa ctctccgcca gatcagataa atgcaaataa
      781 ctaaaccaac gacggtaacc caattaacaa aattttgttg tcctgtgcct aagagaagca
      841 gagcaatctg gacctgattc gcgccttcag aatcccccag ctgtgccagc ctttgcccga
      901 tggtaacaat cctttgagtt cgcagcgggt caagcggggt tccaactcct ctcaggctat
      961 ccaattccgc ccgtaaggtg agcacctctg cacaaatatc acgataagtt tcaatctctg
     1021 caattcgtct ggtatcaccc aattgccgat agagcatcag tgcctgctct gcgctgcgat
     1081 gaccgcgaac ataatcaagg tccaaaatag cctggcgcgc atcaagtgcc aatctcgccg
     1141 cctctttccc aacctgagcc tttgcgaaaa gtgattttgc ctccgaaagc tgttccgggt
     1201 taccaaatag ttcaattaaa gggatcgcgc tttccaaccc ctttgctgca tcctcatacg
     1261 ccccctgggt aatcagttga ccaaactgag atagatcata actgtggatt acgtttgctt
     1321 gccagcggac attgatatac acaggaaaat actcagacca gtgagtttgt aagtcttgca
     1381 ggtttacacc atacacatcg atcaaggcct ggcgataccc ctggttctct gcaatcgcct
     1441 ggaggaactc tacaaaagca tcccaaccaa acacatcaac cagatatcca acgatgctcg
     1501 attgctgtaa cagaacaacg gcattcgaag ctgggatatc ctcatcccac aggcgctgcc
     1561 acccacgatc tggttgaga
//
LOCUS       DBNHOH010000498         2928 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1055532, whole genome shotgun sequence.
ACCESSION   DBNHOH010000498 DBNHOH010000000
VERSION     DBNHOH010000498.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1055532"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            364..1446
                     /locus_tag="AC16V7_08680"
     CDS             364..1446
                     /locus_tag="AC16V7_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257235.1"
                     /translation="MRYLPRQEVSKKIVDAQHPEVTAWRCECRKCGHVFRVYPKGVSQ
                     KQISKRVNGMAVMLYILGLSYGAVEIVLNSLGMGIGKTSVFRAVQAVAKQVPGLKREK
                     LLDGYQTKAVGADVTSVRCNGKWVTIGIAVDAVKGMVLSIDELPGEDAEQLQAWLEPI
                     LDAVDADVVVSDDADAFKQVSDQTGRSQQVCKSHVSRNTDNLVAELAAIIDAGQDRSL
                     EAIGISSQQALEDLASLTEMIHARRPEDQSRLEEIYLRYASARKPGKGKKYDVAYRMR
                     NLFMDRWNLWPRLTFYRNWKDEDGNEILDGTNNHCERAIGWWIKERYRSMRGYKQVQA
                     ALGMSRLIAFAGNNLARGLCLADLMA"
     gene            complement(1607..2760)
                     /locus_tag="AC16V7_08685"
     CDS             complement(join(1607..2488,2491..2760))
                     /locus_tag="AC16V7_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612014.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HIR6257236.1"
                     /translation="MTGRRKFTAEFKAKVVLELISGEKGLMQASREYGIKDSVLSRWK
                     QEFLERAPRVFEQAKSLNPEAERIAELERMVGRLALQLDMAKKVFELELPTQGKRVMV
                     RSLHDWYGCQIGQACELVELPRSSYYYRSHKADESQLVADLEEVSGQYVTYGTRRIRS
                     QLRRPPYAYRINRKRVQRIMRQKGLLRPAKRRKRRTTDSDHPYPRYPNLVKGLGITHP
                     EQVWVCDITYIRLGQGFVYLAIVMDVFTRAIRGWRLSKLLDQELTLAALRMALTDHLP
                     EIHHSDQGVHYAAHAYVDLLKVHQIQISMAAVGKAEENGYAERFMRTIKEEEVDLSDY
                     CDFAEALSQIGRFIEDVYNHKRIHSSLGYLTPAEFEATWLASSPQAGTP"
BASE COUNT          666 a          759 c          812 g          691 t
ORIGIN      
        1 tgctgagatt gagggatgtg gttttatcac gggacatatt gcctcctaat aaagtagtta
       61 gcagagggca atggggaaac agcactccat cagaacttcg atgatatatc aagtggttta
      121 aatagcaagg cgggaaacga attaggagta attttatcat aaaatgctat ttataggggg
      181 tttttagtga tcgttccaaa gctggtagaa acgtggttaa atggttgagg ggcagttagc
      241 ggaccacccc ccaacctaca aacagaaagg cacacgtgca tgcgcctgaa cctaattcta
      301 ccaaaagtcg aaccgacggt atttgaatat ccgaagaaat gcccccggaa aggatgcccg
      361 ggaatgcgat atctgccacg ccaggaagtg agcaagaaga tcgtggatgc tcagcacccg
      421 gaagtcaccg cctggcgctg tgagtgccga aaatgcgggc atgttttccg ggtctacccg
      481 aaaggggtca gtcagaagca aatatcgaag cgagtgaatg ggatggctgt gatgctgtac
      541 attctgggac tgagttacgg tgcagtcgaa attgtattga acagtctcgg catggggatt
      601 gggaaaacga gtgtgtttcg ggccgttcag gccgtggcca agcaagtgcc tgggctgaag
      661 cgtgaaaagc tactggatgg ataccagacc aaagccgtgg gggccgatgt gaccagtgtg
      721 cgctgtaatg ggaagtgggt gacgatcggg atcgccgtgg atgccgtcaa ggggatggtc
      781 ttgagtatcg acgaattacc gggcgaagat gccgaacaac tgcaagcctg gctggagccc
      841 atcctggatg cagtcgatgc ggatgtggtg gtgagtgatg atgcggacgc attcaagcag
      901 gtgagcgatc agaccggacg ttcgcagcaa gtgtgcaaga gccatgtcag ccgcaatacc
      961 gataacttgg tggcggaact ggctgccatt atcgacgccg ggcaagatcg ttctctggag
     1021 gcaattggca tttcgtccca acaagccttg gaggatctgg cttccctgac agagatgatc
     1081 catgcccggc gcccagaaga tcagtcccgc ctggaagaaa tctatctgcg ctatgccagt
     1141 gcccgcaaac caggcaaggg caagaaatat gacgttgcct atcggatgcg caatctgttt
     1201 atggatcgct ggaacctctg gccgcgcctg actttctatc gcaactggaa ggatgaagat
     1261 ggaaatgaga tcttagacgg caccaataac cattgtgaac gcgccattgg ttggtggatc
     1321 aaagagcgct atcgctctat gcgcggctac aaacaagtgc aagccgctct tggtatgagc
     1381 cgcctgatcg cctttgctgg gaacaacctt gcccgtggcc tgtgtttagc tgatttgatg
     1441 gcatagctgt gaagattatt cgccggtttt gccgtcaatg ctgtttacca atccatcttt
     1501 taccaatttt ggaacggtca cattattcga cacaccagtt ttaatcctac tcacccatcc
     1561 tgcctatgtg gtggacccaa aaagttggac aaaaaattgg gtttgcttag ggggtacctg
     1621 cctgcggtga tgatgctaac caggtggctt cgaactctgc cggtgtcagg taacccaacg
     1681 atgaatggat gcgcttgtga ttatacacat cctcgataaa tcggccaatc tgggacaaag
     1741 cctcggcaaa gtcgcagtag tccgacaggt ccacctcctc ttctttgatc gtgcgcatga
     1801 agcgctcagc ataaccgttt tcttcggcct tgcccacggc cgccatgctg atctggatct
     1861 ggtgcacttt cagcaagtcg acataggcat gggcggcgta atgcacaccc tgatcgctgt
     1921 gatggatttc ggggaggtga tcggtcagcg ccatacgcag ggccgccagg gtcagttcct
     1981 ggtcgagcag cttgctcagg cgccagccgc ggatggcacg ggtgaagaca tccatcacaa
     2041 tggccaggta cacgaaaccc tgacccagac ggatgtaggt gatatcacac acccaaacct
     2101 gttcggggtg ggtgattccc agccctttga ccaagttggg atagcgtgga taggggtgat
     2161 cgctgtccgt tgttcgcctt ttccggcgtt ttgccggtcg gagcagccct ttctgacgca
     2221 tgatgcgctg cacccgcttc cgattgatcc ggtaggcgta gggtggacgc ctgagttggc
     2281 tgcgtatccg ccgtgtgcca taggtcacat actgaccaga gacctcttcc aggtcagcca
     2341 ccagttggct ttcatccgcc ttgtggctgc ggtagtagta gctactgcgc ggcaactcta
     2401 ccaactcgca tgcctggccg atctggcagc cataccagtc gtgcaggctg cgcaccatca
     2461 ctcgctttcc ttgcgtgggt agctcgagcg ctcgaagact ttttttgcca tgtccaactg
     2521 caatgccagc cgccctacca tccgctccag ctcggcgatc cgctccgcct ccggattgag
     2581 gcttttcgcc tgctcaaaga cccgcggcgc tcgctccaga aactcctgct tccagcgcga
     2641 taacacgctg tctttgatcc catactctcg gctggcctgc atcaagcctt tttcgcctga
     2701 gatcagttcc agcaccacct tggccttgaa ttccgctgtg aatttccttc gtccggtcat
     2761 cgtgaacctc tttcctggat attaccctac ccctatgggg tctgtccagt ttttggggtc
     2821 cactacaatc atgccttcaa tggaatagac ccattccgat tgcaaattca gtacttcccg
     2881 tgatgtaata agcaacttca catcaggtgc tgcttgaaga agctcatt
//
LOCUS       DBNHOH010000499         1303 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1311324, whole genome shotgun sequence.
ACCESSION   DBNHOH010000499 DBNHOH010000000
VERSION     DBNHOH010000499.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1311324"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..659
                     /gene="ruvA"
                     /locus_tag="AC16V7_08690"
     CDS             84..659
                     /gene="ruvA"
                     /locus_tag="AC16V7_08690"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIR6257237.1"
                     /translation="MISSISGRVQEVNSDNVVIEVGGVGLLVFVPATTRDHLQPGASV
                     FLHAHLVVREDSLSLYGFDSHEGRDFFLLLLGVNGVGPKLALSVLSTLTPDAIRRAVF
                     HEQDEVFARVPGVGKKTAQRILLHLQDRVRMVAGLEPVAGMTDVDTEVMNALTALGYS
                     VVEAQAALQSIPRDAPEELEDRLRLALQYFS"
     gene            679..>1303
                     /locus_tag="AC16V7_08695"
     CDS             679..>1303
                     /locus_tag="AC16V7_08695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase family
                     protein"
                     /protein_id="HIR6257238.1"
                     /translation="MLENQKIAFIGPGVMAEAMISGLIRRNFAQPKMLTAAGPTIERL
                     SHLATKYGIFTDTDNAQAVRNADVVVLSVKPQRLDKVMIGMHGHIKPSALVLSIIAGA
                     SIDKISHGLGHEIVVRSMPNTPAQIGEGITVWTASSQVSERQREVAQQILQALGEEIF
                     VEDEIYLDMATALSGTGPAYVFLFMEAMVDAGVHLGFPRRIAELLVAQ"
BASE COUNT          296 a          299 c          379 g          329 t
ORIGIN      
        1 cgaccagatg acgccgcaga tgccctggca gtggcgatct gtcacctgca ctctttgaaa
       61 atgaaaggca ttatggggtt gtcatgattt ccagtatcag tggacgtgtg caagaggtca
      121 attcggataa tgtggtgatt gaagtgggcg gtgttggctt gctggttttt gtcccagcga
      181 ccacgcggga ccacctgcaa cccggggcgt ccgtgttcct gcatgcgcat ctggttgtgc
      241 gcgaggattc cctgagtttg tatggttttg attcgcatga agggcgagat tttttccttc
      301 tattattggg tgtcaatggc gttgggccca aattggcgct ctccgtgctc tccacgctga
      361 ccccagacgc cattcgccgt gcggtttttc atgagcagga tgaggttttt gcccgtgtgc
      421 cgggagtggg aaagaaaacc gcgcagagaa tactgctgca cttgcaagac cgcgtgcgca
      481 tggtggcggg cttggagcct gttgcaggaa tgacagatgt ggacactgag gtgatgaatg
      541 ccctaactgc actggggtat agcgttgtgg aagcccaggc agccctgcaa tcgatcccac
      601 gcgatgcccc ggaggagctc gaagaccgtt tgagattagc gttgcagtat ttttcgtaag
      661 agtttgtgga ggttagagtt gctagaaaac caaaagatcg catttatcgg cccgggagta
      721 atggcagagg ccatgatctc tggattgatc cgccgaaatt ttgcccaacc caagatgtta
      781 acagccgccg ggccgactat tgagcgttta tctcacctgg caactaaata cggaatattc
      841 acagacactg ataacgcaca agccgttcga aacgcagatg tggtggtgct ttctgtaaaa
      901 ccacagcgct tagataaagt gatgatcggt atgcatggcc atatcaagcc ctcggcatta
      961 gtgttatcca ttatcgctgg agccagcatc gataaaatca gccatggtct tggacatgaa
     1021 attgttgttc gctcaatgcc aaataccccg gctcagattg gcgaggggat caccgtctgg
     1081 acagcatcaa gccaggtaag tgagcggcag cgtgaagttg cacaacaaat attacaggcg
     1141 cttggggaag aaatctttgt agaggacgag atttacctgg atatggctac tgcgctctct
     1201 ggtaccggac ctgcctatgt gttcttgttt atggaagcta tggtggatgc tggtgtgcat
     1261 ttagggtttc cccgtcgcat tgcagaactc ctggtagcgc aga
//
LOCUS       DBNHOH010000500         2956 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1183454, whole genome shotgun sequence.
ACCESSION   DBNHOH010000500 DBNHOH010000000
VERSION     DBNHOH010000500.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1183454"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1735
                     /locus_tag="AC16V7_08700"
     CDS             <1..1735
                     /locus_tag="AC16V7_08700"
                     /inference="COORDINATES: protein motif:HMM:NF037660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR6257239.1"
                     /translation="WYRYHHLFADLLRQRLKRTQPEKLPILHRRASKWHEQNSMGKEA
                     VDHALAAQDFERAAGLIEEQVDAVWQRGEHAKILGWLNMLPKEQFSTRPHLSIFHAWV
                     QLEDGRVKAAELSLRAVEQAIKTNADNEPEKFVYPNSAHLKSRASVMHAMLGFRTADA
                     PSIIKFSTEALESLPEKDLSWRGIAAMALGDSHYIVGDMNGAYKALSEAIRISKTAGN
                     IYMTAYTSIKLAIILQYQGKLHQAQEICRELLTLMYASGLSRSALTGLLHAQWGEILW
                     DLNDVDNAFQYVSRGLELSKLENDVANLVWAHIVLVKVLYAKRDLTGAMDTILKLEKI
                     ARKSHVPPWIVNRVAAWKARIWLTERNLVAIDHWVQGRQLNKDDELIFAREVEYIVLA
                     RILIMKGELYDAVNLLERLIEKAEAGDRITRLIEMLLVLALAKNAQGDIDNAVVTLLK
                     SLSLAASGGHIRIFVDEGPSIEALLRRVKVENGKIKGHISTLLATFEEKITGSPSRTQ
                     PLIEPLSERELEVLHLIAQGLTNQEISSRLYLSLNTVKVHTRNIYGKLGVNNRTQAVA
                     RANGLGILLYA"
     gene            1916..2599
                     /locus_tag="AC16V7_08705"
     CDS             1916..2599
                     /locus_tag="AC16V7_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257240.1"
                     /translation="MEKATPQINLSNGELKMKKQKIGLSLFWIGVIFALAFAAIGTRS
                     LMHNLRNLTAEELDATIWAMGDPLFILWALSVTLGSVLAGIGAFAYVKTKQAFSWLTG
                     IGVLGAVIVMVMVWSRVYNATLFGIGGIIILVSFFAIVWVWIKKYAALESEEKTAGSY
                     KLIGYIFWINASWFLCGETAKMHLRAFEGQSAPSPIEIIVFLILGWVFVLIGEFKEMR
                     LTRKKAVSL"
     gene            2688..>2956
                     /locus_tag="AC16V7_08710"
     CDS             2688..>2956
                     /locus_tag="AC16V7_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002169108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR6257241.1"
                     /translation="MKAIVYTKYGPPEVLQLTKVRKPAPKENEVLIKVFATTVTKYDC
                     WMRSCTAPPGFGLLMRISSGRKPKQPILGTELAGEIDWVGKDVTR"
BASE COUNT          830 a          628 c          737 g          761 t
ORIGIN      
        1 ttggtatcgc tatcaccatc tatttgcaga tttgcttcgt cagcgcttaa agcgaaccca
       61 accagaaaag ttgcccatcc tccatcgccg tgccagcaaa tggcatgaac aaaatagcat
      121 gggtaaagag gcagtagatc acgcattggc agctcaagat ttcgaacggg cggcgggcct
      181 gatcgaggag caggtcgatg ctgtttggca gcggggagag catgcaaaaa tattgggatg
      241 gctgaacatg ttgcccaagg aacagttttc caccaggcca catctctcta tttttcacgc
      301 ctgggtacaa ttggaagatg gcagagttaa agcagcggaa ttgagcttac gggcagttga
      361 gcaagcaatt aaaactaacg cagacaatga accagagaaa tttgtctatc caaactctgc
      421 acacctaaaa agcagggctt ctgtgatgca cgccatgctg ggttttcgca cagcagatgc
      481 tccaagcatc atcaaattct ctaccgaagc acttgagtct ctacccgaaa aggatttgtc
      541 gtggcgcggc atcgctgcga tggcactagg tgattcgcat tatattgttg gcgacatgaa
      601 tggggcctac aaagccttgt ctgaggcgat cagaattagt aaaactgcgg gcaatatcta
      661 catgaccgca tacaccagta tcaaacttgc cataattctt cagtatcaag gcaaattgca
      721 tcaagcccag gaaatatgtc gagagttatt gacgcttatg tacgcaagcg gattatcgcg
      781 gtcagcacta actggtttgt tacacgccca atggggtgag attctatggg acctgaatga
      841 tgttgataac gctttccaat atgtgagtag gggtcttgaa ttgagcaaac tcgaaaatga
      901 tgtggctaat cttgtatggg cacacattgt attggtgaaa gttttatatg caaagagaga
      961 tttgaccggg gcgatggata caatcctgaa attggaaaaa atcgcaagaa aatcccatgt
     1021 tccgccctgg attgttaacc gggtagcagc ctggaaggca cgcatttggc ttaccgagag
     1081 aaatcttgtt gccatagatc attgggttca ggggcgacaa ctgaacaaag atgatgaact
     1141 catatttgcg cgcgaggttg agtatattgt tcttgcccgc attctcataa tgaagggtga
     1201 attgtatgat gcggttaatc tgcttgaacg actgattgaa aaagctgaag caggtgaccg
     1261 cattacgcga ctgatcgaaa tgttactggt tctggcattg gctaaaaatg cacaaggaga
     1321 catagacaat gctgtggtca ccttgttgaa gagcctttca ctcgccgcat cgggaggcca
     1381 catccgtatc tttgtggatg aaggcccgtc gatagaggca ttattaagaa gggtcaaagt
     1441 cgaaaatgga aaaattaagg gacatatcag cacgctgctc gctacctttg aagaaaagat
     1501 cactggttct ccatctcgca cccaaccgct gattgagccg ttaagcgagc gtgagctgga
     1561 ggtgcttcat ctcatcgccc agggactaac caatcaggaa atctcttcca ggctttacct
     1621 gtcactaaac acagtcaagg ttcacactcg gaatatctac ggaaaactcg gtgtcaacaa
     1681 ccgaacacaa gctgttgcca gggcaaatgg attggggatt ttgctctatg cttgattacc
     1741 agaaggtctg aattcggcac tggttacaaa caataacaaa gaatactgac ggggcactaa
     1801 acagtggccc gtttttgttt ccttttccac tttgaaatta accctctgaa taacactttc
     1861 ggggtatgac acttatacac ccggttgcta tactgtttgt gcaagaattc acttcttgga
     1921 aaaagcaact cctcaaatca acttatccaa tggagagcta aaaatgaaga aacaaaaaat
     1981 tggtcttagc ttattctgga ttggcgtaat ctttgcctta gcattcgccg ctattggcac
     2041 gcggagtctt atgcacaacc tcaggaatct tacggctgaa gaacttgacg cgaccatttg
     2101 ggcgatgggc gacccattgt tcatactctg ggctctatct gtgactctgg gttcagtact
     2161 ggcgggcatt ggcgcctttg cgtacgtgaa aaccaagcaa gctttttcgt ggctcactgg
     2221 tattggggtc cttggtgcag tcattgttat ggtcatggtt tggagccgtg tatataacgc
     2281 aacgctgttt ggcattggcg gcatcattat cttggtttcc ttttttgcaa tcgtgtgggt
     2341 atggataaaa aaatatgccg cgcttgagag cgaagaaaaa accgcaggga gctataagct
     2401 catcgggtat attttctgga taaatgcttc ctggttcctg tgtggagaga ctgcgaaaat
     2461 gcacctaagg gcgtttgaag ggcagtctgc gcccagccca atagagatca ttgtttttct
     2521 aatcttgggt tgggtctttg tgctgatagg tgaattcaaa gaaatgcgtc tgacgagaaa
     2581 aaaagctgta agcctctgag ctggattgga gcatcactga ttgactaggg cttatatgat
     2641 ttcactaatc tgcaaaaagc agaaaaatga tgactaaaaa tgacgcaatg aaagcgattg
     2701 tttacacaaa atacgggcct cctgaggttc ttcaactcac aaaggttaga aaacctgctc
     2761 ccaaggagaa tgaggtactg atcaaagtat ttgctacaac tgtaaccaaa tacgactgtt
     2821 ggatgcggag ttgtaccgcg ccgcccggtt tcgggctatt gatgcgaata tcatcaggga
     2881 gaaaacccaa gcaacccata ctgggcactg agctggccgg ggaaattgac tgggttggca
     2941 aagatgttac ccgatt
//
LOCUS       DBNHOH010000501         5792 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1311380, whole genome shotgun sequence.
ACCESSION   DBNHOH010000501 DBNHOH010000000
VERSION     DBNHOH010000501.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5792)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1311380"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="AC16V7_08715"
     CDS             <1..991
                     /locus_tag="AC16V7_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257242.1"
                     /translation="LDVAGGGPLPTTNLLEQIEIPANVNPKLLEFSMDLALQEDERFD
                     EVGAAGEVLWFLNRLEPEAVLEPPVYLRYPGIDYDRSLLTIEMEALERELDDELSPLE
                     GRPQDFEEVQLNLIYPHWRAGTLPLSSRLRYQFPTAYEAPRIRFTLVDGLSGEKFPAW
                     VVRPKRYVYGLREWYETHNLVPGSRIRIQRSQVPGEVIIYPDTHRPSKEWVRTVLIGV
                     DGGVVFAMLKQTIYSKFDERMTIAVPDVEAVDRLWAQMSKERQPFEKVVVNTVRELAK
                     LNPQGHVHASELYAALNVIRRCPPGPILTLLASRPWFTHVGDLHFRFNDTERS"
     gene            1003..1437
                     /locus_tag="AC16V7_08720"
     CDS             1003..1437
                     /locus_tag="AC16V7_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257243.1"
                     /translation="MVEVKRVSLVKPALQTPFHIDFNWWAKNDRDWRVYLRSNLCIEH
                     QLAFEHVDLEEKVDWVDPVTAEVQQVDGLQNILITHCAKQPGFITEHSTLVDAAFRLF
                     LASGNAPMSAVELGERLGRPPETILRTFTGGRVYKGIRPCME"
     gene            1452..1524
                     /locus_tag="AC16V7_08725"
     tRNA            1452..1524
                     /locus_tag="AC16V7_08725"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1485..1487,aa:Arg,seq:tct)
     gene            1596..2117
                     /gene="tpx"
                     /locus_tag="AC16V7_08730"
     CDS             1596..2117
                     /gene="tpx"
                     /locus_tag="AC16V7_08730"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005835444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HIR6257244.1"
                     /translation="MSIVRKGLFEFRGQDAPVVGADIQVNQQAPEFTVQGMDWSLFRG
                     MGDTQGKVRIIGSLPSLSTSVCDRETRRFNQEAAALGEDIVIMMVSMDLPYTIKNWCG
                     AAGVDRVVTVSDHLSGEFGEKYGVLLKDQRIFRRAIFVVDRNGKVVYAEYMPKMGDEP
                     NYEQVLTVAKDVL"
     gene            2186..2608
                     /locus_tag="AC16V7_08735"
     CDS             2186..2608
                     /locus_tag="AC16V7_08735"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR6257245.1"
                     /translation="MINVVLKPDVQRPGFYILQDVRKLPEATRQRMAVRSHAWRPPTD
                     VFETEEMVIVRVEIAGMEETDFTIVLDGRYLHIRGVRSDVPERRAYHQMEIRFGDFSS
                     EVELPSDISLEHIEAVYSVGFLHIRLSKVRPVKIKVED"
     gene            2693..5272
                     /gene="lon"
                     /locus_tag="AC16V7_08740"
     CDS             2693..5272
                     /gene="lon"
                     /locus_tag="AC16V7_08740"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR6257246.1"
                     /translation="MPASRWTSNFLNPLNLDDQDDLTLEDLLLKQIVPQVIRPGEKDD
                     ESLPEDDETGPVIPSVLPILPLRGLVVYPQTAVPLTIGQPRSIRLIDDVVAGENRLIG
                     LVASRDPELDTPEPKDLFNIGTAAMVHRLFRAPDGTIRLVIQGLARFHVGDYVQLEPY
                     LKANIELIPEKVDEGAELEALARSALNQFDRIAEMIPSIPRELVASILALEDPLQTVY
                     TIANFQRMELEDAQEILEIDSASDKLHKLVGILAHEVEVLEIGQKIQNEARSEIEKVQ
                     REYFLREQLKAIQKELGEGDEQAAEVEEFRRKIEAAKMTEEAEKQARRELDRLSRLPT
                     AAAEYGVIRTYLDWLVSLPWSVITEDILDINHASEVLDQDHYGLEDVKKRILEYLAVR
                     KLRLERSEELAAEPLDDIRRVREGVILCFIGPPGVGKTSLGRSIARAMGRKFIRISLG
                     GLRDEAEIRGHRRTYIGALPGRILQALRRIESRNPVFMLDEVDKLVFDFHGDPASALL
                     EVLDPEQNSEFRDHYLEIAFDLSQVMFITTANSLETIPGPLLDRMEVIRLSGYTESEK
                     IAIAQGYLIPRQLKENGLRTSEVVFLRPALEKIIHSYTREAGVRNLEREIGSVCRKVV
                     TRIAQGKSETEEVTPEKVQELLGRPKFYATEEVALRTSIPGVATGLAWTPVGGEVLFV
                     EATSMPGNKGFQVTGSIGNVMQESARAALSFVRSRANNLGLNPEFFEKSDIHLHVPAG
                     SQPKDGPSAGVTMATALVSLVCGCPVRADLGMTGEITLRGQVLPIGGVKEKVLAAHRI
                     GLKTVVLPVRNEADLEELPEEVRKDIQFIFAETVDDVLRAALESPPEKPKRRSKKKPE
                     EHQ"
     gene            5280..5645
                     /locus_tag="AC16V7_08745"
     CDS             5280..5645
                     /locus_tag="AC16V7_08745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6257247.1"
                     /translation="MPKVSLIEDDASMLSLLGTLLGFEGFEVAPLDGGMNLEETLENL
                     RRENPQLILLDVHLPAISSFDLLQRLRQDPELKSVQVLMSSGMELTDKCLQAGADDFI
                     LKPYMPDELIGKIRKMLKH"
BASE COUNT         1424 a         1341 c         1589 g         1438 t
ORIGIN      
        1 cctggatgtg gcagggggag gtcccttgcc caccaccaac ctattggagc aaatcgagat
       61 tcctgccaac gtcaacccga agctgcttga gttctctatg gatctcgcgc ttcaggaaga
      121 tgagcgcttc gatgaagttg gcgcagccgg tgaggtgctt tggttcctaa accgccttga
      181 gccagaggct gtgctggagc cgccagttta cttgcgttat ccagggattg attatgatcg
      241 ctcgttgtta accatcgaaa tggaagcgct cgagcgcgaa ctggatgatg agctttcacc
      301 attagagggg aggccccaag attttgaaga agttcaattg aatctgatct accctcattg
      361 gcgggcaggc actctgccac tttcatcgag attgcgctat caatttccta cagcttatga
      421 ggcgccgcgc atccgcttta ccttggtaga tggccttagt ggtgaaaaat ttccagcctg
      481 ggtcgtgcgc cctaaacggt atgtttatgg gttgagagaa tggtacgaaa ctcacaattt
      541 agtaccagga agcagaatac gcatccagcg cagccaagtg ccaggggaag tgataattta
      601 cccagacaca caccgtccga gcaaggagtg ggtgcgcacc gtgttgatcg gtgtggatgg
      661 cggggtggtt tttgccatgc tcaagcaaac gatttattcg aaatttgatg agcgcatgac
      721 catcgccgtg ccagatgtgg aggcagtcga tcgcctttgg gctcagatga gcaaggagcg
      781 tcaacctttc gaaaaggtgg ttgttaacac cgtgcgcgag ctggcgaagt taaatccaca
      841 gggtcatgtg catgccagcg agctatatgc tgccctgaat gtgattaggc gctgtccacc
      901 tggacccatc ttgacattgc ttgcatcccg tccttggttt acacatgtag gcgatttaca
      961 ttttcgcttc aacgataccg aacggagtta gtggagggaa atttggtcga agtcaaacgt
     1021 gtcagtctcg tcaaaccagc gctgcaaacc ccctttcaca ttgatttcaa ttggtgggcg
     1081 aaaaatgacc gggattggcg tgtttatttg cgtagtaatt tgtgcataga acaccaatta
     1141 gcatttgaac acgtagattt agaggaaaaa gttgattggg tcgatccagt aactgctgaa
     1201 gtacagcaag tagacggctt gcagaatata ttgatcaccc attgtgccaa acaaccagga
     1261 ttcataacag aacactcaac cttggtcgat gcagcattcc ggctcttcct cgccagcggc
     1321 aatgccccga tgagtgccgt ggagttggga gagcgcctcg gtcgccctcc ggagacgatc
     1381 ttgagaacct ttaccggcgg tcgtgtctac aagggcattc gtccgtgcat ggaataatcg
     1441 acagcatgct tgcccccgta gctcaaagga tagagcacct gccttctaag cagttggctg
     1501 tgggttcgaa tcccgccggg ggcgcctgat tgtcattgat agcaatttgt ttgttatctc
     1561 ttttgttcaa cagcgaattc taagggagaa aattaatgag tattgttcgg aaaggtttgt
     1621 tcgaatttcg tggccaggat gccccagtgg ttggtgcaga tattcaggta aaccagcagg
     1681 cgcctgagtt tactgttcag gggatggatt ggtctctttt taggggaatg ggtgataccc
     1741 aaggcaaggt gcgcattatc ggttcgctcc cctcgctgag tacgtcggtt tgtgatcgcg
     1801 agacgcgccg tttcaaccag gaagctgctg cgctggggga ggatattgtc attatgatgg
     1861 tgagtatgga tctgccttat acgatcaaaa attggtgtgg tgcagcaggg gttgacaggg
     1921 tggtcaccgt gtcggaccat cttagtggag agtttggcga aaagtatggc gtattattaa
     1981 aagatcaacg gatatttcgc agggcaatct tcgtcgtcga tcgtaacggg aaggttgttt
     2041 atgcggaata tatgccgaaa atgggtgatg aaccaaacta tgagcaggta ttaacggtag
     2101 ccaaggacgt tctttaagca tggggtttca ggtattcgat tgaaggctgg ttgaatgatc
     2161 ttcaattgga aaccaaggtg gtcatatgat aaatgttgtc ttaaaacctg atgtgcaaag
     2221 acctggtttt tacattttgc aagatgttcg aaaactccca gaggcaaccc gccagcgtat
     2281 ggcagtgcgt tcgcatgcat ggcgcccacc cacggatgtg tttgaaaccg aagagatggt
     2341 aattgtccgc gtggaaatcg ccggtatgga ggagaccgac ttcacgattg tcttagatgg
     2401 gcgttacctc catattcgcg gagtacgcag cgatgttccc gaacggcgcg cttaccacca
     2461 gatggaaatt cgcttcggtg attttagcag cgaagtggag cttccatccg atatttcttt
     2521 ggagcacatc gaggcggttt acagtgtagg ttttctgcat attcgtttat ctaaggttcg
     2581 cccggtaaag attaaggtcg aagattagag tgaatctacc cggatgactg cttcatcaga
     2641 agagaaattg atcatgaggc gctcgtcgga tccagaaaat aaaagagata aaatgcccgc
     2701 atctcgttgg acgtccaact ttttaaaccc tctcaatctg gatgatcagg atgacctaac
     2761 tctggaagat ttgctattga agcagattgt cccccaggtt atccggcctg gagagaagga
     2821 tgatgaatcc ctaccagagg acgatgagac tggacctgta atcccgtctg ttcttccaat
     2881 tctgcctttg cgcggtctgg tggtataccc tcagactgcg gtgccgctaa cgattggaca
     2941 gccacgttcc attcgactga tagacgatgt cgttgctgga gagaatcggc ttattgggct
     3001 ggtagcctcc cgagatccgg aacttgatac cccagaaccc aaggacttat tcaatattgg
     3061 gaccgcagcg atggttcacc gcttatttcg ggcgccggat ggcaccatcc gtctggtcat
     3121 tcagggtttg gcgcgctttc acgtgggaga ttatgtccaa ctagaaccct acttgaaagc
     3181 caatattgag ctaattccag aaaaggtaga tgagggagca gagttagagg ctctggcgcg
     3241 tagcgctctt aaccagtttg atcgcattgc agagatgatc ccctcaatcc ctagggagtt
     3301 ggttgcatcc attctggctt tggaagaccc gttacagacg gtgtatacca ttgcgaattt
     3361 tcagcgcatg gagctagaag atgcgcaaga aatcctagaa atagattcgg cttccgataa
     3421 actgcataag ctggttggta tcttagccca tgaggtcgag gttcttgaga tcgggcaaaa
     3481 aatccagaat gaagcccgtt cagaaattga aaaagtgcag cgagaatatt tcctgcgcga
     3541 gcagcttaag gccattcaga aagagcttgg tgaaggcgat gagcaggctg cagaggttga
     3601 agaatttcgc aggaagatcg aagccgccaa gatgactgag gaggccgaaa agcaagcccg
     3661 tcgggagcta gaccgtcttt cgcgcttgcc aactgctgca gccgaatatg gggtcattcg
     3721 aacttatctt gattggctgg tttctctgcc ttggtcggtg atcacagagg atattctgga
     3781 tatcaatcat gcgagcgagg tcttagacca ggaccattat ggcttggagg atgtcaaaaa
     3841 acgcattttg gagtacttgg cggtacgcaa gctgcgattg gagcgctccg aagaactggc
     3901 agctgagcca cttgatgata tccgccgcgt gcgtgagggc gtcattctgt gcttcatagg
     3961 tcctccaggg gtgggcaaaa cctccctcgg tcgatccatt gcacgagcta tggggcgaaa
     4021 atttatccgc atttccttgg gaggcctgcg tgatgaagcc gagatccgcg gccaccgccg
     4081 gacttatatc ggcgctctgc ctgggcgaat cctacaagca ctgcgtcgta tcgagtcccg
     4141 aaacccggta tttatgctgg atgaagtcga taagctggtt ttcgatttcc acggcgaccc
     4201 ggcttcggct ttattggagg tgctcgaccc agagcaaaac tcagaatttc gcgaccatta
     4261 cctggagatc gccttcgatc tttctcaggt aatgttcatc accactgcca attcccttga
     4321 aaccatacct gggccgctac ttgaccgcat ggaggtcatc cgcctgtccg gttataccga
     4381 gagtgagaag atagccattg ctcagggata cctgatcccc cgccaattaa aagaaaacgg
     4441 tctgcgcaca tcggaagtgg ttttcctccg ccccgcgttg gaaaaaatca tccactccta
     4501 cacccgcgaa gctggcgtgc ggaatcttga gcgagagatc ggatctgtct gccgcaaagt
     4561 agttacccgc atcgctcagg gcaagagcga aacggaggag gtgactcctg aaaaggtaca
     4621 ggagctactt ggacgcccga agttttatgc cactgaggag gtagccttac gcacctcgat
     4681 accaggcgtg gcgactggtc tggcttggac acctgtagga ggcgaggtgc tctttgtcga
     4741 ggccaccagc atgcccggca ataaaggttt tcaggtaacc ggctcgatcg gcaacgtgat
     4801 gcaggagtca gctcgggctg ctctttcgtt tgtacgctcg cgcgccaaca accttggctt
     4861 aaaccctgaa tttttcgaga aaagcgacat tcatctccat gttccggccg gctcgcagcc
     4921 aaaggatgga ccatcggctg gtgtgaccat ggcaactgct ctggtttcgc tggtgtgtgg
     4981 ttgccccgtg cgcgcagatc tgggcatgac gggtgagatc accttgcggg gtcaggtatt
     5041 gcctataggt ggtgtgaagg agaaagtatt ggcggcgcac cgcatcggat tgaaaactgt
     5101 ggtgctacca gtccgcaatg aggctgacct ggaggagttg ccagaagagg tgcgtaaaga
     5161 tatccaattt atctttgctg aaacggtaga tgatgtgctg cgcgctgccc tggaatcccc
     5221 tcctgagaaa cctaaacgga gaagcaaaaa aaagcctgag gagcatcaat aagatcatca
     5281 tgcccaaagt atccttaatt gaagacgacg cctccatgtt atccctttta ggtactttgt
     5341 tgggatttga gggatttgaa gttgccccat tggatggtgg aatgaatctc gaagagactc
     5401 ttgagaactt gcgcagagag aatccccagc ttatcttact tgacgttcat ctgcctgcaa
     5461 ttagcagttt tgacctgttg caacggctgc gccaagatcc tgaattgaag tctgtgcagg
     5521 tgctgatgtc ttcgggtatg gaactgactg ataaatgcct tcaagctggg gcagacgatt
     5581 ttatccttaa accatatatg cccgatgaac tcatcggaaa gatccgtaaa atgctcaaac
     5641 attaacccaa aaatgggtaa atcaagaaag tacaccgata aaatatggca aagaaaaaaa
     5701 caagcgccgt gatgcaatgg cgcaccatgg atttgcattt acacactcca gcttctagcg
     5761 atttcttaca gcctggggtt agctacctag ag
//
LOCUS       DBNHOH010000502         2546 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_606582, whole genome shotgun sequence.
ACCESSION   DBNHOH010000502 DBNHOH010000000
VERSION     DBNHOH010000502.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_606582"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(385..1086)
                     /locus_tag="AC16V7_08750"
     CDS             complement(385..1086)
                     /locus_tag="AC16V7_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257248.1"
                     /translation="MPILQDIEFSITADQILQAQGANPALIRKRKPNLVLTAERALDI
                     GLPLLEPLVLYRELELEKVRHETLILSEGYTLKSQLLTQHLAYAKIIVVILCSIGEKM
                     EKEIASLYDSDPVLGLALDGVGSAAVEALANATCKFFEARAQESKWQASIPLSPGMTS
                     WPVDEGQPEIFHILDPSSIDVRLTSSYLMLPRKSLTMVLGFGPQLMISGTTCSYCAMQ
                     ETCKYQDHYQESILP"
     gene            1242..1745
                     /locus_tag="AC16V7_08755"
     CDS             1242..1745
                     /locus_tag="AC16V7_08755"
                     /inference="COORDINATES: protein motif:HMM:NF046293.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR6257249.1"
                     /translation="MLDDIDRSIISLLQYDGRTPHTKIAEELDINEGAVRRRVKKLID
                     EGIMQIVAIVEPHQLGWSEAAMIGITVNPNMIDEAATAISQLQEVTYLFQAAGEFDLF
                     AEVYCKNRAHFVSFLNNRLQKIPGIERTQTFLILKMYKLSYRWGETEAPLVKHTLTPQ
                     STEPTRK"
     gene            1759..>2546
                     /locus_tag="AC16V7_08760"
     CDS             1759..>2546
                     /locus_tag="AC16V7_08760"
                     /inference="COORDINATES: protein motif:HMM:NF018012.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR6257250.1"
                     /translation="MNWNQSSDDFVELIHQKSAEILAEVGFCAPDKTMLSRLMAAGFP
                     VNEDTHMVRIPEELMKAALEALPKDVKLYYRGSQQAVDFGRGSLFMGAGTPVSVQDLK
                     TGDRRDATRSDVRNLVTIQDALPQVDIVRPTVTATDMGACSDLVEIAELLMHTEKPVV
                     HRTLSPDRVEAAVEMLAAVAGGYEALRAYPNFATLYCPISPGYFTPENIRCMLKWAEF
                     GIPITLLSMAMGGASAPATLLGELLVINTDILAWIVVLEILYPGT"
BASE COUNT          659 a          556 c          636 g          695 t
ORIGIN      
        1 ttgtgcttcg gggacctgca tgctcataca ggtccccgaa gtacaaatca tattggactg
       61 gtttttgtgc ttcctttgct gaagtgacca aaccgaaact gaatgatcca aggataatga
      121 ttgaaaggag cactattcgg atttttttac tcatggctac cctcccggtt tgaggattct
      181 tgatgtggag ttctttcggc caatacgaat aacaacaaac gattatgggt ttttgtgaaa
      241 aggttggttc gatttttagt gaacctaatg acttcgactg gatctgctga acggactgaa
      301 tgtcagttgc gatgaagtga agctcaataa taattttcgc tggttatttc cctagccggg
      361 atggtgtgat gcctggtgct ttgcctacgg tagtattgat tcctgataat gatcttgata
      421 tttacaagtt tcctgcatag cgcagtagct acaggtggtg ccactgatca tcagctgggg
      481 accaaagccc aacaccatgg tgagcgattt gcgggggagc ataaggtaag acgaggtgag
      541 ccttacgtca attgagctgg gatccaggat gtgaaatatt tctggctgac cttcgtcaac
      601 gggccagctt gtcatgccgg gacttagtgg aatcgacgcc tgccacttgc tttcttgggc
      661 gcgggcctca aaaaatttgc aggttgcatt ggctagcgct tctaccgctg ctgaaccgac
      721 tccgtctagt gccaacccca agaccggatc gctatcgtat aggctagcga tttccttttc
      781 cattttttcg ccaatgctac acaggatcac aacaataatt ttggcatatg ccaagtgttg
      841 ggtgagcagc tggctcttta gtgtatatcc ctctgaaagt atcaatgttt cgtggcggac
      901 tttttccagc tccaactctc gatagagcac aaggggctcg agcaatggaa gaccgatgtc
      961 aagagccctt tctgctgtca agacaagatt gggtttgcgc ttcctgatta acgccggatt
     1021 tgccccttgg gcctggagga tttgatcagc agtgattgag aattcaatat cttgcaggat
     1081 aggcaagttc tacagtctcc tcaaagagtg tgtataaatt atagcgaaca aaatgatgca
     1141 agtcaatcta aaaacggcgt tattcgtaaa atttgctaat aaaatttacg aataacgata
     1201 aaatttattg actgttttac gaattacggt aaaatataag tatgttagat gatattgaca
     1261 gatccataat ttcgctcctg caatatgatg gtcgcacccc acatactaaa atcgccgagg
     1321 aattggatat caacgagggg gccgttcggc gtagggtgaa aaagctcata gatgaaggga
     1381 tcatgcaaat tgtcgccatc gttgagcctc atcaattggg ttggagcgag gccgcaatga
     1441 ttgggataac tgttaatccg aatatgatcg atgaggccgc cactgcaatt agtcaattgc
     1501 aagaagtaac ctatttgttt caagcagcag gcgaatttga tctgtttgca gaggtctatt
     1561 gcaagaatcg agcgcacttc gtttcttttc tcaataacag gcttcaaaaa atccctggca
     1621 ttgaacggac ccagacattt cttattctca aaatgtataa attatcatat cgctggggtg
     1681 agacagaggc gccgctcgtt aaacacacac ttacgcctca atccactgag ccaacgcgca
     1741 aataattaag agatgatgat gaattggaac caaagctcag atgattttgt agaacttatc
     1801 caccaaaaat ctgccgaaat ccttgctgag gttggtttct gcgcccctga taaaacaatg
     1861 ctatcgcgcc ttatggcagc tggtttcccc gttaacgaag atacccacat ggttcgcatc
     1921 cctgaggagt tgatgaaggc ggcacttgag gcgctcccca aagatgtgaa gctctattat
     1981 cggggcagtc aacaagccgt agattttgga cgtggatctt tattcatggg cgctggcacg
     2041 ccggtcagcg tgcaggacct aaaaaccggg gaccgccgtg atgccacccg aagtgatgtg
     2101 cgtaacctcg taactataca ggatgctttg ccacaggttg atatcgtccg gccaacggta
     2161 acggcaactg atatgggtgc gtgctccgat ttggtagaaa ttgctgaact tttgatgcat
     2221 acagaaaagc cggtggtcca ccgtacgcta tcaccagacc gcgttgaagc ggcggtagaa
     2281 atgttggcag cagtagctgg gggatatgag gctttgcggg cgtatccaaa ctttgctacc
     2341 ttgtactgtc ccataagccc gggttatttc acgcccgaga atatccgctg tatgctcaaa
     2401 tgggctgagt ttgggatacc gattactttg ctgagcatgg cgatgggagg ggcttctgct
     2461 ccggctactc ttttggggga actgctggta atcaatacag atatattggc ctggatcgtc
     2521 gttctagaga tcctttaccc tggcac
//
LOCUS       DBNHOH010000503         3389 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1119215, whole genome shotgun sequence.
ACCESSION   DBNHOH010000503 DBNHOH010000000
VERSION     DBNHOH010000503.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1119215"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="AC16V7_08765"
     CDS             complement(<1..913)
                     /locus_tag="AC16V7_08765"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR6257251.1"
                     /translation="MRKTIVRLLYGFIGLLILAVLAITALIGYDFLFPSQRITDFANV
                     TYPGPQGTTLNGYLARPGDGEPGPALLLIHAFYGLDQDIIQKADLLAQQGYTVLAVDA
                     FRGQTTRRVPRGIWLVVTTPQDEIRADIDAAYTYLAGLESVDPQRIGVVGFCFGGSQA
                     MFLGTRNPDLRATVIFYGGGPITDPAELGFLGESGPVLGIYGAEDQSIPLEQVSAFEA
                     AMNTRGISNQISVYPGVGHAFVHADNIAQPGAAQDAWNEMLTFLSENLGSGVTWDGSG
                     RAQSPDVPGADKRLATSGFHLNHALYHV"
     gene            complement(1054..1368)
                     /locus_tag="AC16V7_08770"
     CDS             complement(1054..1368)
                     /locus_tag="AC16V7_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HIR6257252.1"
                     /translation="MTMWLVMLAAGLLTYLTRLSFILLWGKINIPIWLLRSLRFVPPA
                     VFTAVIFPELFLPQGTLDISLGNARLIAGILAALVAWKTRNIVLTILVGMSVLLLIQY
                     FV"
     gene            complement(1365..2066)
                     /locus_tag="AC16V7_08775"
     CDS             complement(1365..2066)
                     /locus_tag="AC16V7_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HIR6257253.1"
                     /translation="MTSPRTEFLSGVRDELPLLVGVIPFGMIYGILAVSAGIPAVQAQ
                     AMSLILFAGSSQFITAQLFGHGTPAMVMVLTAAIVNLRHALYSASIAPYTRKLRPAWK
                     WTLAYLLTDEAYAVSIHHYQSHDSHSNPESTGNKHWYLLGAGLGLWSTWQVSTAMGVF
                     IGAQVPASWSLDYSLVLTFIALLVPNLRDRPSLAAALAAGVTAVLAHPLPFKLGLVCA
                     ALVGIITGVWLESRK"
     gene            complement(2181..>3389)
                     /locus_tag="AC16V7_08780"
     CDS             complement(2181..>3389)
                     /locus_tag="AC16V7_08780"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6257254.1"
                     /translation="ASKVRDTGVQRASLGASFFVIVGLISAMGTALVYGIGGYLVIQG
                     DFTIGTIVAFGAYLTSLYSALQGLANAPVEFATSIVSFERVFEVIDLPMDIVEKSEAR
                     VLSNISGELIFEEVSFRYEVDEQVELSAVRRFGQMDSVTTVLSGERPEHHPRDDEGNE
                     DVPRSQARVIALDKISFRVEPGQLVALVGPSGAGKTTLTYLIPRLYDPTEGRILIDGH
                     DLRQVSLDSLSAQIGMVTQETHLFHDTIRTNLLYARLEATQDELEAAARAANIHDFIA
                     GLPDGYDTIAGERGYRLSGGEKQRIALARVILKDPRILVLDEATSHLDSESELLIQEA
                     LKRVMSGRTSIVIAHRLSTILAADLILVMDRGRFVERGTHTELLALNGLYASLYETQF
                     SREKDQIQKA"
BASE COUNT          770 a          936 c          987 g          696 t
ORIGIN      
        1 atacgtggta gagggcgtgg ttcagatgga agccgctggt ggcgagcctt ttatccgcgc
       61 cgggaacatc tggactttgc gccctgccag acccatccca ggtaacccct gatcccaggt
      121 tctccgacaa aaaggtcagc atctcgttcc aggcgtcttg tgctgcgccg ggttgagcga
      181 tgttgtccgc atgaacgaag gcatgtccaa cacccggata aacgcttatt tggttagaga
      241 tgcctctagt attcatggcg gcctcaaaag cacttacctg ctccaagggg atgctttggt
      301 cttccgcacc gtagatgccc agcaccgggc ctgattcccc caagaaaccc agttccgcag
      361 ggtcggtgat cggtccgcca ccgtaaaaga tgactgtggc gcgcaggtcc gggttgcgtg
      421 tgcccaggaa catcgcctgc gagccgccga agcaaaagcc taccacacct atccgttggg
      481 ggtctacgct ctccaatcct gccaggtacg tgtaggctgc atcaatgtcg gcgcggattt
      541 cgtcttgcgg agtcgtcact accaaccaga tgccgcgcgg gacacggcgg gtggtctggc
      601 cgcgaaaagc gtcgactgcc aggacagtgt atccttgctg agcaagcaag tcggcttttt
      661 ggattatgtc ttggtcaagc ccataaaaag cgtggatgag cagcagcgct ggacctggtt
      721 cgccatcacc aggacgggct agatagccgt tcagggtggt tccttgcggg ccgggatagg
      781 tcacattggc gaagtcggtg atgcgctgag atggaaacaa aaagtcatag ccgatcaagg
      841 cggtaatcgc aaggacagcc aggatcagca aaccgataaa cccataaagc aggcggacga
      901 tcgttttacg cataaaatct ctccaatgcc aatcgctggc ttgccagcgt gttggataat
      961 ggctgttatt gtactctatc gtaaagtttt ccctgacctt accccctttt tcatgtgttt
     1021 acctgagata gtcaatgccg agctatttgt gtcctaaaca aaatactgga tgagcagcag
     1081 cacgctcatg cccactaata tggtgagcac gatattgcgt gtcttccagg cgaccagcgc
     1141 agccaggatg cccgcaatca ggcgggcatt gcccagggaa atatccaggg ttccctgcgg
     1201 gaggaacaac tcggggaaaa tgacggctgt gaatacagcc ggggggacaa agcgcagcga
     1261 gcgcagcaac cagatgggga tgttaatctt accccacagc aggatgaagg aaaggcgggt
     1321 gaggtaggta agcaagcccg cagccagcat gaccagccac atggtcattt tctactctcc
     1381 aaccacacgc cagtgattat gcccaccaga gcggcacaaa ccaagcccag tttaaagggt
     1441 agcgggtgcg ccaggactgc cgtgacccca gccgccagag cggccgccag gctagggcgg
     1501 tctctcaagt taggcaccaa tagggcgatg aatgtgagca ccagggagta gtccagcgac
     1561 cagctggcgg gaacctgggc gcctatgaag acgcccatcg ctgtgctcac ttgccaagta
     1621 gaccacaaac ccaaccctgc acccagcaag taccagtgct tattccccgt ggactctggg
     1681 ttactgtgac tgtcatggct ttgatagtgg tgaatgctca ccgcgtaagc ttcatcggtg
     1741 agcaagtaag ccagggtcca cttccaggcg ggtctcagct tgcgggtgta tggtgcgatt
     1801 gaggcgctat acagtgcgtg ccgaaggttg acgatagctg ccgtcagcac catgaccatg
     1861 gcaggcgtgc cgtgcccgaa caattgggcg gtgatgaact gcgaagagcc cgcaaacagg
     1921 atgagtgaca tagcttgcgc ctgcaccgcc gggatgccag ccgagacagc caaaatgcca
     1981 tagatcattc caaaggggat tacacccacc agaagcggta gctcatctct gactcccgag
     2041 agaaattccg tccgaggcga tgtcatttct caccccagat ggggtaagag aagagggatg
     2101 tggggatgag agacgaagta cctttttgca cttaaaccta actgggattc ggatgggatt
     2161 ggacagatcc tgaaggctac tcaagccttt tggatttgat ctttctcacg gctgaattga
     2221 gtttcgtata ggctggcgta caagccgttc agggcaagca actcggtatg ggtgccgcgc
     2281 tccacgaagc gacccctatc catcaccagg atcagatccg ctgccaggat cgtgctgagg
     2341 cggtgggcga tgacaatact ggtgcgccct gacatgacgc gcttcagggc ttcctggatg
     2401 agcaattccg attcgctgtc cagatggctg gtagcctcgt cgagcaccag gatgcgcggg
     2461 tctttcaata tcactcgcgc cagtgcaatg cgctgtttct cgccgccgct caaacgataa
     2521 ccacgctctc cggcaatggt atcgtaacca tccggcagcc cagcgataaa atcatggata
     2581 ttagccgccc tggccgcggc ttctaattcg tcttgggtcg cctcaaggcg agcgtagagc
     2641 aggttggtgc ggatggtgtc gtggaagaga tgggtctctt gggtgaccat accgatctgg
     2701 gcggagagcg agtccaggga tacctggcgc agatcatgcc catcgatcag gatgcgccct
     2761 tcggtggggt cgtaaagccg agggatcaag taggtgaggg tggtcttgcc tgctccgctg
     2821 gggcccacca gcgccacgag ttgtccgggt tccacgcgaa agctgatctt gtccagtgca
     2881 atcacgcgcg cctggctgcg cggtacgtct tcattccctt catcgtcgcg agggtggtgc
     2941 tccggcctct cgccggaaag aacggttgtc acgctatcca tttgcccgaa ccggcgcacc
     3001 gcgctgagtt ctacctgctc atccacttca taacggaagc tcacttcttc aaagatcaat
     3061 tcaccggaaa tgttggataa gacacgcgcc tccgactttt ccacgatatc catgggtagg
     3121 tcgatgacct caaagacgcg ctcaaagcta acgattgagg tggcaaactc aaccggtgcg
     3181 ttggccagac cttgcaaggc gctgtagagg ctggttaggt atgcgccgaa ggccacgatg
     3241 gtaccgatgg tgaaatcccc ttggatcacc aagtaacccc cgataccgta taccagggct
     3301 gtacccatgg cagagatcag gccaacgatg acaaagaagg atgcccctag cgaggcacgt
     3361 tggacgccgg tatcgcgcac cttggatgc
//
LOCUS       DBNHOH010000504         4463 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_93958, whole genome shotgun sequence.
ACCESSION   DBNHOH010000504 DBNHOH010000000
VERSION     DBNHOH010000504.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4463)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_93958"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1135)
                     /locus_tag="AC16V7_08785"
     CDS             complement(29..1135)
                     /locus_tag="AC16V7_08785"
                     /inference="COORDINATES: protein motif:HMM:NF024873.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HIR6257255.1"
                     /translation="MSTPLGDTYVVESRYPLDYAQGASLLWTDQPLNLLASWAQDERI
                     NDLAPQAFAFLDTETTGLSGGTGTYAFLIGVGRIQSGEFYLTQFFMRDPVYEPGQLAA
                     LEEFIAPCSALVTFNGKSFDMPLLHTRYLSHGWIPPFSDQAHIDLLHLARRLWRDRLP
                     SRTLPNLELQILGTTRTEEDVPGWMIPQIFFDYLRSGDARPLKSIFYHNAMDVLSLAA
                     LLNHIINMLAEPLNGQVEHSLDIIAMAKLFEEQGFTDLAIQLYLQGIDIGLPEEAAIN
                     TMLRLATIHKRQENFAKAIPLWEQAAKQQQLHAHVELAKYYEHRAQNFQQAIFWTESA
                     LSVVYDPNFPKHEKPFWKSELEHRLKRLHRKHHG"
     gene            complement(1347..3944)
                     /locus_tag="AC16V7_08790"
     CDS             complement(1347..3944)
                     /locus_tag="AC16V7_08790"
                     /inference="COORDINATES: protein motif:HMM:NF012492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR6257256.1"
                     /translation="MSIPRLLSRWRTDPSIGSCFEVWHKLDERQGQFDPLPGNLHPHL
                     VSALLSQGISSLYTHQSLSWNLVQQGHHIVVVTGTASGKTLCYNLPVLDKLLREPLSR
                     ALFLFPTRALAQDQRSTLNEFLAHAPDSETQIPLAVYDGDTPSSSRPAIRKKARLVIS
                     NPDMLHAGILPHHTSWAEFFNQLRFVVIDEIHAYRGVFGSHVANVLRRLKRIATHYGS
                     SPQFILTSATIANPKELAELLVESRVDLVDNDGSSRGSRHFLMYNPPVINRELGLRRS
                     ALQESVRLAEDLLAYNVQTIIFGRSRRTVELILTYLRQRTATDHTSESDKDSASRISE
                     PIRGYRSGYLPIQRREIERGLRMGDVRLVVATNALELGIDIGKMGASLLVGYPGTIAA
                     TWQQAGRAGRGEDTALSVLISTADPLDQFLVRHPDYLFERPPENALINPDNPLILLDH
                     LRCAAFELPFRDGENFGSVEASKVGEFLDFLQESGILHYSGGRFFWMSEQYPAQETSL
                     RSASANNVILQTMIDDSPLTIGEVDHGSAHWMVHPGAVYIHEAQTYLVESLDLEQSIA
                     SLQLVETDYYTEPRIETNVILEEKFGESLVQGASKAHGAIQVTAQLTGYRKLRWYTHE
                     LLAFSELALPPTKLLTTGYWIALQESTIERLREVGLWLNDPNQYGPNWRQQRDRARAR
                     DSYRCQVCGTPEGERVHDVHHKIPFRAFTDERGSIDEQAANRLENLITLCPSCHRRAE
                     TAVRVQSGLAGLAFTLRHLAPLFLMCDTRDLGVHADPQSPLGDGQPVVVIYDQIPAGI
                     GFSQRLFELHDELLARARELVLTCECGEGCPSCVGPGGEHGMGGKKETLAILEALVLE
                     DNNLEEIVH"
     gene            complement(4128..4418)
                     /locus_tag="AC16V7_08795"
     CDS             complement(4128..4418)
                     /locus_tag="AC16V7_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR6257257.1"
                     /translation="MKKFVFLTYGYETPTQEIMDAWSNWFASIGDKILDSGNPFGPGK
                     EITHEGIKELPHNKEAITGYMIINAENIDAAVQIAKDCPIITSIRVYEAMSM"
BASE COUNT         1126 a         1033 c         1134 g         1170 t
ORIGIN      
        1 gttataccgc aaattggaat acatttgctt atccatgatg tttacgatgc agacgcttta
       61 ggcgatgctc taattcactt ttccagaagg gtttttcatg ttttggaaaa ttgggatcat
      121 acacaaccga gagtgcagat tcggtccaaa agatggcttg ctgaaaattt tgggcgcggt
      181 gctcataata tttggctaat tcgacgtgcg cgtgcagttg ttgctgttta gcagcctgct
      241 cccatagggg aattgctttc gcaaagttct cctggcgttt atggatggtt gccagtcgta
      301 acatcgtgtt tatagcagcc tcttctggca aaccaatatc tatcccttgg agatatagct
      361 ggattgcgag atcggtaaaa ccctgttctt caaacaattt agccatggcg atgatgtcta
      421 aactgtgttc aacctgaccg ttaagcggct ctgctagcat gttgattatg tggttgagca
      481 gcgctgccag cgagagcaca tccatggcat tatgataaaa aatgctcttt agaggacggg
      541 catcaccgct acgaaggtaa tcgaagaaaa tttgagggat catccaccct ggcacatctt
      601 cttctgtacg cgtagtcccg aggatctgaa gttccaggtt agggagtgtc cggctgggta
      661 gccggtcacg ccacaagcga cgggccaggt ggagcaaatc gatgtgtgct tgatctgaaa
      721 agggtgggat ccaaccatgg gacaaataac gtgtatggag caaaggcata tcaaaagact
      781 tgccattaaa ggtgaccaag gccgagcagg gagcgatgaa ctcttcgaga gcagcgagct
      841 ggccaggttc gtataccgga tcccgcataa aaaattgtgt taggtaaaat tctcctgact
      901 gaatacgtcc tacaccgatc agaaatgcat aggtgcctgt tccacccgat aggccagttg
      961 tctccgtatc gaggaatgcg aaggcttgcg gtgcgaggtc gttaatgcgc tcatcttgcg
     1021 cccacgaggc cagtagattt aggggttgat ctgtccataa aagcgatgcg ccttgcgcat
     1081 agtcaagagg atagcgcgac tcaacgacat aggtatcccc aagaggggtg ctcaatatgc
     1141 gtccattcag tacacgttct atcgaatagg gattagttgc cgggggaggt tttagatctt
     1201 ttgccccgac ttggacgcca agggatttta attgatcaga aagggagggc atagagaatg
     1261 tgaattggtg gataaaacca aatttgtgcg ttaggggaag tttaccataa atcatccgat
     1321 tcccttgagg tggaacactt cccaaatcaa tgcacaatct cttctaaatt attatcttca
     1381 agtacgaggg cttccagaat agccaaagtt tcctttttcc cccccatacc atgttcccct
     1441 cccgggccca cacaagatgg acagccttct ccacactcac aggtgagaac aagttctctg
     1501 gcacgcgcga gcaattcgtc gtgtaactca aaaaggcgtt ggctgaagcc aatgccagcc
     1561 ggaatttgat cgtagatcac aaccacgggt tgtccatccc ccagaggtga ttgaggatca
     1621 gcgtgtacac ccagatcgcg tgtgtcacac atcaggaaca atggagccag gtgcctgagc
     1681 gtaaaagcca atcccgccaa accgctctgc acccgtacag ccgtctctgc ccgcctatgg
     1741 caggacggac agagagtgat cagattttcc agccggttag cagcctgttc gtcaatcgat
     1801 ccacgttcat ctgtgaatgc tcgaaaggga attttatggt gcacatcatg gacgcgctcg
     1861 ccttctggag taccgcaaac ctggcagcga taggaatccc tcgcacgagc tcgatctcgc
     1921 tgctggcgcc aattcgggcc gtattgattt gggtcgttta accaaagtcc gacttcccgc
     1981 aggcgttcga tggttgattc ttggagtgct atccagtatc cagtggttaa taactttgtg
     2041 ggtggtaggg caagctcact gaatgcaagc agctcatgtg tgtaccatct gagcttacga
     2101 taacctgtca gctgagcggt gacctgtata gcaccatgcg ctttggatgc gccttgaacc
     2161 agagattctc caaatttttc ctctagtatt acgttcgttt caatccgtgg ctcagtgtaa
     2221 taatctgttt ccaccagttg taaagaggca atactttgtt ctagatctag ggactcgacc
     2281 agataagtct gagcttcatg gatatagaca gccccaggat gaaccatcca atgggcgctg
     2341 ccatggtcaa cctctccaat ggtcaggggg gaatcgtcga tcatagtttg taggatgaca
     2401 ttatttgctg atgcgctccg caaagatgtt tcctgggcag gatattgttc tgacatccag
     2461 aagaaacgcc cacctgaata gtgcaagatg cctgattctt gaagaaaatc caagaattcg
     2521 cccaccttac tagcttcaac gctaccaaaa ttttctccat cccgaaaagg tagctcgaat
     2581 gctgcgcatc gcaagtgatc taatagaata agtggattat ccggatttat cagggcgttt
     2641 tctgggggac gttcaaagag ataatctggg tggcgtacca gaaactggtc tagcggatcg
     2701 gcggttgaga taagtacgga tagggcggta tcttcgcctc gaccagcacg tccggcttgt
     2761 tgccaggtag ctgcaattgt gcctgggtaa ccgactaata gcgatgcgcc cattttcccg
     2821 atatcgatgc ccagttccaa agcattggtg gcgacaacca ggcgcacgtc acccatgcgc
     2881 aagccgcgtt ctatttcgcg acgctggatt ggcaggtaac ctgatcgata gccccgtata
     2941 ggttcagata ttctcgatgc tgaatctttg tccgattccg aagtgtgatc ggttgctgtg
     3001 cgttgcctaa gatatgtgag gatcaactca actgttcgcc gcgaacgccc aaagatgata
     3061 gtttggacgt tataggcaag caagtcctca gccaggcgga cactctcctg caatgcactg
     3121 cggcgcaatc ctagctcgcg gttaatcaca ggagggttat acatcaaaaa atgccttgat
     3181 ccgcgtgagg atccatcatt atctaccagg tcaacgcgag attcaaccag tagctctgca
     3241 agctctttag ggttagcaat cgtcgcagaa gtgaggatga attgagggga tgagccataa
     3301 tgggtagcga tgcgtttcaa gcggcgtaac acgttggcaa catgcgatcc gaaaacacca
     3361 cgatatgcat ggatctcgtc tatgactaca aagcgtagtt ggttgaaaaa ctctgcccag
     3421 gaagtatggt gaggtaagat gccagcgtgc agcatatctg ggttgctgat gaccagcctg
     3481 gctttcttgc gtatggcggg acgagacgac gagggtgtat caccatcata aaccgcgaga
     3541 ggaatttggg tttcggaatc aggggcatga gcgagaaatt cattcaaggt cgagcgttgg
     3601 tcttgagcta gagcccgggt tgggaataag aagagagcgc gagatagagg ttcgcgcagc
     3661 aatttgtcga gaaccggaag gttgtagcag agtgtttttc cgctggccgt gccggttacc
     3721 acaacgatat gatgcccttg ttgaaccagg ttccaggata acgattggtg agtgtatagc
     3781 gagctgattc cttgagacag caatgcggag accagatgtg gatgaagatt ccctggcaac
     3841 ggatcaaatt ggccttgacg ctcatctagc ttgtgccaga cctcaaaaca acttccaatg
     3901 gatgggtcgg ttctccacct gctcaataac ctgggaattg acatatcatc atgcttggac
     3961 cgcgccagat atctcttttg tgtcaaaatg gctgcgcatt cgcactcgta ggagaatctt
     4021 aagaaagatc aaccattttt aggtgattta taactcgtta tagaggcgaa tgttccgcct
     4081 cgctccaatt ttagtttaga tttatcccgc ttaataaaca aatctcatta cattgacatc
     4141 gcttcgtata ctcttatgct tgtaattatt ggacaatctt tagcgatttg taccgctgca
     4201 tcaatgtttt ctgcgttaat gatcatgtag ccagttatcg cctccttgtt atgtggcaat
     4261 tctttgatcc cttcatgcgt gatttctttt ccaggtccaa atgggttgcc gctatcaagg
     4321 atcttgtctc ctattgaagc gaaccaattg ctccaggcat ccattatttc ctgggtgggt
     4381 gtttcatatc cgtaggttaa aaatacaaat ttcttcattg ttcgtcctta cattattttg
     4441 tgggatgtga aaattcacca tag
//
LOCUS       DBNHOH010000505         3420 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1184049, whole genome shotgun sequence.
ACCESSION   DBNHOH010000505 DBNHOH010000000
VERSION     DBNHOH010000505.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1184049"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..1575)
                     /locus_tag="AC16V7_08800"
     CDS             complement(46..1575)
                     /locus_tag="AC16V7_08800"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF012589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HIR6257258.1"
                     /translation="MDYVIGCDVGSQAVKAVLLSTEGQILAEAGASYDISFPRPAWAE
                     QDSNSWIQGVVQAISKLVREAGVRREQIRAIGLDAQVDGFVPIDAAGQPLHAGIIWMD
                     RRAVEQTESIRKQYDSSRLFELSGLNLDPYHVAPKICWFAETHPELYEKSRYFMLPGS
                     YVAYKLSGEYGVDYSNASSTLLMDVRKKEWSTELCNYFQIAIDKLTPIYPANSVIGSL
                     LPEMAEQMGLDAQTKVILGCGDEHAASLGAGVIIPGLVCDIAGTAEPIGAASQQPLFD
                     ETGLVETHCHADPDLWFLENPGFVSGGNYRWLRDQFARDQELEATKLGIDIYDLLNRE
                     AENVPPGSDGLILLPCLSGAMTPTWNAYARGVFFGFTLAHQRGHFIRAVLEASAYALR
                     DITDRMQAMGLALTQIRAVGGGARSPLWRQIKADVTGLPIALLQTVETTALGAGLLAL
                     AGMGYTRDLTEAVELAVRVVETRDPNPQTGEIYEAYYQLYRAAYFAMLPIFEQASKIS
                     P"
     gene            complement(1599..2300)
                     /locus_tag="AC16V7_08805"
     CDS             complement(1599..2300)
                     /locus_tag="AC16V7_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005949627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/alpha barrel domain-containing protein"
                     /protein_id="HIR6257259.1"
                     /translation="MKPELIVMLTHQDMTVKNAKELFEECKDLPVTHWGFKDVGLPHE
                     QMKELVRRMKVEGKVTYLEVVSLSEEDGLVGAKLAVEAGFDILMGTVYFDSIHQYLKD
                     KPIKYYPFPGHVHSHPSILDGTIEEIVNHAISLQSTGVQGLDLLAYRYVGDARKLLLD
                     VVKATNIPLVCAGSIATFQRIAEVWEANSWGFTIGSAFFDMKFVPGGSFKENIEAVTD
                     WLAKTDEADVPGYLR"
     gene            complement(2340..3221)
                     /locus_tag="AC16V7_08810"
     CDS             complement(2340..3221)
                     /locus_tag="AC16V7_08810"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR6257260.1"
                     /translation="MEETITTSTVQEEIYKPRSAQTDRLLRWLTYLLTAFIIILFLFP
                     WLWVFSLGFKTRTEILSPNIVWIWKPTLDNFNEVLLVKGYWRLILNSLLVAASAVLLC
                     MIIGVPTAYAFSRFPIPGKESWFYMILTFRMAPAVVLALPLYVLFARTRLLGTYFAPI
                     IAHITFTLPFVIWLMRSFFEDIPKEIDAAAMTDGYTRAGAFFYAVLPLVKSGIVAVTL
                     FSIIFSWNEFLYGLILTSDTTRPVASQIPSLIRPHGTLWGEVCALATAASLPVAFLIF
                     ALQRQLVRGLSFGAVKG"
BASE COUNT          899 a          860 c          831 g          830 t
ORIGIN      
        1 catccatatc aaggaggaaa cactggattt cattgagtgg acgtctcatg ggcttatttt
       61 tgaggcttgc tcgaaaattg gcagcatagc gaaataggca gcgcggtata gctggtaata
      121 tgcctcataa atttcgcctg tctggggatt ggggtcgcgg gtttcgacca cgcgcacggc
      181 aagttccaca gcctcggtca aatcgcgtgt ataccccatg ccagccagag ctaacaggcc
      241 agcgcccaat gcggtggttt ctactgtttg gagcaaagca attgggagac ccgttacatc
      301 ggcttttatt tgccgccata gtgggctgcg cgctccgcca ccaacagctc gaatttgagt
      361 taatgctaaa cccatcgcct gcatccggtc ggtgatatcc cgcagggcat aagcggatgc
      421 ctctaatact gcccggataa aatggcctcg ctggtgggcg agggtaaatc cgaaaaacac
      481 acctcgggcg tatgcattcc aggtgggcgt catcgcccca gaaaggcaag ggagtaagat
      541 caatccatcg ctgcccgggg gtacgttttc cgcttcccgg ttgagtagat cgtagatatc
      601 tataccgagc ttggtcgcct ctaattcctg gtcgcgagcg aattggtctc gtaaccagcg
      661 ataatttccc ccggatacaa agcccgggtt ttccaaaaac cataggtctg gatcagcatg
      721 acaatgcgtc tctaccaggc cggtttcatc gaagagcggc tgctggcttg ctgcaccaat
      781 tggttcggct gtgccggcga tatcgcatac caaccctgga ataatcacgc ctgctcccaa
      841 gctggcggcg tgctcgtcac cgcaacccaa aatcactttg gtttgggcgt ctaaacccat
      901 ctgctcggcc atctccggaa gaaggcttcc tattacgcta ttggcagggt agatcggcgt
      961 gagtttatca atggcgattt ggaagtaatt gcataactca gtggaccact cttttttgcg
     1021 cacatccatt aacagtgtcg aagatgcatt ggaatagtcc acaccgtatt cccctgatag
     1081 cttgtaggcg acgtaactgc cgggtagcat gaaatatcgc gatttttcgt acagctcggg
     1141 gtgggtttcg gcaaaccaac atatttttgg agctacatga taaggatcaa ggttcaggcc
     1201 agaaagctcg aataatcggg atgaatcata ctgtttccgg atggattccg tttgctctac
     1261 cgcgcgacga tccatccaga tgatccctgc atgcagtggt tgaccagcgg catcaatggg
     1321 tacgaagccg tccacctggg catccagacc aatggcgcga atttgctctc ttcggactcc
     1381 agcctctcta acaagctttg agatcgcctg tacgacaccc tgtatccaag agttagaatc
     1441 ttgctctgcc caggctgggc ggggaaagga tatgtcgtaa ctggctcccg cttcggcgag
     1501 gatctgcccc tccgtagaga gcagaacagc ctttacagct tgagagccga catcacaacc
     1561 aattacataa tccataagtg ccttggggtt ggaaagcgtc aacgcaaata accagggaca
     1621 tccgcttcat ccgtttttgc caaccaatcg gttaccgctt caatattctc cttaaatgag
     1681 ccacccggaa cgaatttcat atcgaagaat gcactaccta tggtaaatcc ccatgaattt
     1741 gcttcccaaa cttcggcaat acgctgaaaa gtggcaatac tccctgcaca gacgaggggt
     1801 atgtttgtgg ctttaactac atctagcaat aacttgcggg cgtcacccac gtaacggtag
     1861 gctaataagt cgagcccctg gactccagtg gattgcaaag agatggcgtg gttgacaatt
     1921 tcttcgatcg tgccgtccaa gatcgaagga tgactgtgga cgtgacctgg aaatggataa
     1981 tacttaatcg gtttatcctt caaatattgg tggatgctgt caaagtaaac cgttcccatt
     2041 aggatgtcga agccagcttc aactgcgagc ttcgcaccaa ctaatccgtc ttcctctgag
     2101 aggctgacca cctcaaggta agtgactttc ccttctactt tcatccgacg aacgagctct
     2161 ttcatctgct cgtgaggcag cccaacatcc ttaaagcccc aatgggttac tggcaagtct
     2221 ttgcattctt cgaagagctc ttttgcattt ttaacggtca tatcctgatg cgtgagcatg
     2281 acaattaatt ctggtttcat cgtcaatccc tccaaataat ctacccagcc atggtgaatt
     2341 tagcctttaa ctgctccaaa agataaccca cgcaccaact gtctttgaag ggcgaagatc
     2401 aagaatgcca cgggcaggga ggctgcggtt gccagggcgc aaacctcccc ccaaagcgtg
     2461 ccatgtgggc gtatcaatga tgggatctga gaggcaaccg gtcgcgtggt atcgctagtc
     2521 agtatcaaac catacagaaa ttcattccat gaaaatatta ttgagaacaa ggtcactgca
     2581 actatgccag atttgacgag tggaaggaca gcgtaaaaga atgcgccagc gcgtgtatag
     2641 ccatctgtca tagcagccgc atctatctcc tttgggatat cttcaaaaaa gcttctcatc
     2701 agccagatta cgaacggtaa tgtaaatgtg atatgggcaa taattggagc aaaatatgtt
     2761 cccaataatc ttgtccgggc gaagagcacg taaaggggca aggctaaaac aaccgctgga
     2821 gccatgcgga atgtcaatat catgtagaac cagctctctt tgcccggaat tgggaaacgt
     2881 gagaatgcat aggcggttgg cactccaata atcatacata gaagaactgc tgaagcagcc
     2941 accaatagag agttgagaat caaccgccag tatcctttca ctaatagaac ttcgttgaag
     3001 ttatccaaag tgggtttcca gatccacacg atgtttggac taaggatctc ggtgcgagtc
     3061 ttaaagccaa ggctgaacac ccacaaccag ggaaagagga acaagatgat aataaacgct
     3121 gtcagcagat aggtaagcca ccgcaataat cggtctgttt gagcgctgcg gggcttatag
     3181 atctcttctt gaaccgtgct tgttgtgatt gtctcttcca tgaggtcact cctttacaaa
     3241 ggattaggcg ctgctgctta ccccttgcca gcgctgaaga gctttataga gatactctac
     3301 aagaatcgaa atgaaaacaa aaattaccat aatcaacgct gagccatagc ctaagtccca
     3361 atagcgaaaa gtatggcggt aagcgtagac tgctacagtt tcggtaactg tccctggacc
//
LOCUS       DBNHOH010000506         1924 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_479001, whole genome shotgun sequence.
ACCESSION   DBNHOH010000506 DBNHOH010000000
VERSION     DBNHOH010000506.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_479001"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="AC16V7_08815"
     CDS             complement(<1..792)
                     /locus_tag="AC16V7_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6257261.1"
                     /translation="MPIESIEALQEALRQKLYIAERGLATSIYLAHKLSRPLFLEGDA
                     GVGKTEIAKVMANILDTELIRLQCYEGLDVHHAVYEWNYTRQMMAIRLMEAQGEHTSE
                     DELFSNKFLLRRPLLQAIESKNGKPPVLLVDEIDRSDEEFEAFLLEVLSDFQITIPEI
                     GTIRASEPPFVVITSNRTREVHDALKRRCIYYWIDYPPFEKELQIVQMKIPQASSNLA
                     RQITQFIQELRQADLYKIPGIAETIDWTTALVALDQTALDESIIQE"
     gene            complement(935..1207)
                     /locus_tag="AC16V7_08820"
     CDS             complement(935..1207)
                     /locus_tag="AC16V7_08820"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR6257262.1"
                     /translation="MTGTFGGALEARGIPAGEGYLTTQIRGEVGKDEKVLIIRRIHVT
                     YTLKIADEHRETAQRVHGFHAQYCPVARSIRDCIEISTELQMEPLS"
     gene            complement(1208..1411)
                     /locus_tag="AC16V7_08825"
     CDS             complement(1208..1411)
                     /locus_tag="AC16V7_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257263.1"
                     /translation="MTGDTVYTSEIRIERIKGPHRLAYLPVTPAPVHFGVHSEIAEHY
                     NIDSNVHNPYAATIDYVIAATGG"
     gene            complement(1567..>1924)
                     /locus_tag="AC16V7_08830"
     CDS             complement(1567..>1924)
                     /locus_tag="AC16V7_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="4-diphosphocytidyl-2C-methyl-D-erythritol
                     kinase"
                     /protein_id="HIR6257264.1"
                     /translation="MLEQRIREDGNLILLAGETAIMDRYDIAPRAVERAGGQVECVGV
                     PVDPGNLLMLGYIGPVPVLGVPGCARSKKTNIVDWVLPRLLAGDRLARYDFMRLGHGG
                     LLEDTRQRPMPRNTVF"
BASE COUNT          432 a          497 c          505 g          490 t
ORIGIN      
        1 ctcctggatg atcgattcat ccagagcggt ttggtctaat gctaccaggg cggtggtcca
       61 atcgatcgtt tcagcaatcc cagggatttt atataaatca gcctggcgca gttcctggat
      121 aaactgagtg atctgccggg ccaaattgct ggaagcctgg gggattttca tttgaacgat
      181 ctgcagctct ttttcaaagg gagggtaatc gatccaataa taaatgcagc gccgtttgag
      241 tgcatcgtga acctcccgtg tccggttgga ggtaatgacc acaaagggcg gttcgctggc
      301 gcggatagtg ccaatttcgg ggatggttat ctgaaaatcg gagagcacct cgagcaagaa
      361 agcttcaaat tcttcatcgc tccggtcgat ctcatcgaca agcaaaaccg ggggcttgcc
      421 gtttttgctt tcaatcgcct ggagcaaggg ccgccgcagc aggaatttat tgctgaacaa
      481 ttcgtcttcc gaggtgtgct ctccctgggc ctccatcagc cggattgcca tcatctgccg
      541 ggtgtagttc cactcataca cagcatgatg aacatccaag ccttcgtagc attgcaggcg
      601 gataagttca gtgtccagga tgttggccat gaccttggca atctcagttt tgcctacgcc
      661 agcatcacct tccaggaaaa gcgggcggga gagtttatgg gccaagtaga tcgaggtcgc
      721 cagaccccgt tcggcgatat acaatttttg gcgcagggcc tcttgcaagg cttcaattga
      781 ttcaatgggc atgacttgat gatactcgat attttctata ggacaagatc tgtttttctg
      841 gttcagtaat cgttaggata ggtgatctaa atttcaatcc aacgacggtc aactaggatt
      901 cgaatagtcg gatattagag cctcagggtc tcgattacga gagtggttcc atctgcaatt
      961 cggtcgagat ttcgatgcaa tcccttatgg accgcgccac cgggcagtat tgtgcgtgga
     1021 aaccatggac gcgctgggca gtctcgcggt gttcgtctgc gatttttagc gtataggtta
     1081 cgtgaatgcg acgaataatc agcaccttct cgtccttgcc tacttcccct ctgatttgtg
     1141 tggtgagata accttcacct gccgggatcc cgcgcgcttc cagcgcgccg ccgaaggtcc
     1201 cggtcagtca gccaccggtt gcggcgatca cataatcgat cgtcgcagcg tacgggttgt
     1261 gcacgttcga gtcgatgtta tagtgctccg caatttcaga atgcacgcca aaatggactg
     1321 gggccggtgt cacaggcagg taggcaagcc ggtggggacc cttgatgcgc tcaatgcgga
     1381 tttctgaggt ataaacggta tcacctgtca tatattttta tggtctggac ttgaatacaa
     1441 gctaagatca gcctctgttt tcgttatttg ccaacaggat cctaagggtt ctcattacga
     1501 ataatgagca tggtattgga tgaaaaccct taggatcttt gttaatcgta ccttctattt
     1561 tattggttaa aatacagtgt tccgcggcat aggcctctgg cgggtatcct ccaacaaccc
     1621 gccgtgaccc agacgcataa aatcatatcg cgccagcctg tctccggcca acagcctggg
     1681 cagcacccaa tctacaatat tggttttctt actccgtgca caacccggca ctccgaggac
     1741 gggtaccggg ccgatataac ccagcatgag caggttgccg gggtcgaccg ggacccccac
     1801 gcattcgacc tgtcccccgg cgcgctccac cgctcgaggg gcaatatcgt agcggtccat
     1861 gatcgccgtt tcaccggcga gcaaaatcaa attcccatct tcgcggatgc gctgctcgag
     1921 catt
//
LOCUS       DBNHOH010000507         4239 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_94075, whole genome shotgun sequence.
ACCESSION   DBNHOH010000507 DBNHOH010000000
VERSION     DBNHOH010000507.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_94075"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="AC16V7_08835"
     CDS             complement(<1..393)
                     /locus_tag="AC16V7_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010437249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257265.1"
                     /translation="MKTFLSILLALTLLLTACAQATPTEAPPEATELPAVEATEAPPQ
                     PPSAEGYTPPEGAMPPVPLAEGSQSTTPVAYKAGDPIKIAYMPPATEFNYYMAIGEGI
                     KALAAEAGVETFMLAPQSGSDIATQMKML"
     gene            complement(490..1476)
                     /locus_tag="AC16V7_08840"
     CDS             complement(490..1476)
                     /locus_tag="AC16V7_08840"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6257266.1"
                     /translation="MERSTTELRKAPTWLAQLVAFRELSLILVLLIFGIFMSLVSPVF
                     LTWPNLEAILLSLSVEATIAVGMVILLISGGLDLSVGSTLGLTGVVAGLVLTAGFPAP
                     VAILSGLLAALGVGLVNGLLVSKMKINPFITTLGMMITIRGLLLVLAQGRAVLNLAQS
                     FTVIGQGRLFSVQYPIFVMLALVLAGDLLMRNARFFRQSYYIGGNEKAARLSGINVDL
                     VKIINYCIVALLAGVAGLLITARFGSASVTVGSGVELRVITATIIGGASLNGGEGSVF
                     GAFLGALFMAVLANALNLMGVDVYWQNLVTGLILIIAVVVDVINERRKSLQR"
     gene            complement(1524..3056)
                     /locus_tag="AC16V7_08845"
     CDS             complement(1524..3056)
                     /locus_tag="AC16V7_08845"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR6257267.1"
                     /translation="MEDVLRAEHICKRFPGVLAVDHVSLSLRRGEILALVGENGAGKS
                     TLMQILGGALRPDEGQILLEDRPLVFNSPEDAIRSGISMVFQELSLVGSLSVAENIFV
                     NRQPVGALNVIRWGELYRQTADFLDRFELDLNPRWLVKQLSMGQQQILEILKAISTNP
                     KVLILDEPTSSLTESEIAYLFQNIRRLQAQGMSFIYITHKLSEVFQIADRVMVMRDGK
                     YVDSKPIRDVTENNLVAMMVGRQITHLYGARTDKDRDEYFRVEGFSRRGSFQDVSFSL
                     RRGEILGFAGLVGAKRTEVGRAIFGIDRRDSGRILLNGNELNIRKPEDAIRYRIAYLT
                     EDRKGQGLFLSMSLRDNLVAPSFNKFTSRLGFVRHPLLERYAGEMVRQFDIVTPSMEK
                     KVLNLSGGNQQKTLIAMWVGIQPEVIIFDEPTRGVDVGARAEIYARLRDFAATGVGVI
                     MISSDLPELIGMCDRILVMHQGRITGEVGREDFSEALILSFAAGLEQVVRSMDNRNAQ
                     AQ"
     gene            complement(3132..4160)
                     /locus_tag="AC16V7_08850"
     CDS             complement(3132..4160)
                     /locus_tag="AC16V7_08850"
                     /inference="COORDINATES: protein motif:HMM:NF024799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR6257268.1"
                     /translation="MKNKMYILIGFLVAISMLAAACAQQPAAPASEAQPESAQPATAD
                     EEYIYVSAMGNLEFFNAHKYGWKWAGERLGVTATYVGPAEYDMNAMVAAFDQAIAKKP
                     KGICTFAVEPVLTPSINKATEAGIPVVTILGDLPESKRIAFVGSRQYDLGFTGGVKIA
                     EALGGKGKVAILSIPGVGMFDDRETGFRAAFEAYPDIEVVQVGDTKADTVTAVSVAKD
                     IMQRYPDLAAFVGTDSTGGIGAATAVEEAGKVGQVKTVAMDRNSDVLEKIQKGTLTGT
                     VAQDDAAMAFWCLQTLYNYVHNQAPLTTDNAKAGASTGPTNVFMSVNYIDASNLQYFL
                     DANALYTK"
BASE COUNT          953 a         1195 c         1145 g          946 t
ORIGIN      
        1 gagcatcttc atctgggtag cgatgtcgga gccggactgt ggggcaagca tgaaggtttc
       61 cacgcctgct tcggctgcca gcgccttgat gccctcgccg atcgccatgt agtagttgaa
      121 ctcggtggcg gggggcatgt aggcgatttt gatgggatcg ccagctttgt aggctactgg
      181 agtggtggat tgcgatccct ctgccagcgg cacaggcggc atggctccct ccggtggggt
      241 gtagccttca gccgatggtg gctgaggtgg cgcctcggtc gcttccactg cgggcagttc
      301 ggtcgcttca ggcggggctt cggttggtgt cgcctgtgcg caggcagtca acagcagggt
      361 tagcgctaga agtatggata gaaaggtttt catctcaggt actcctccgt ttttatttgt
      421 gtgagtctgc ttttattagg aatctacttg aattagcata ccgggtcatt ttttctgggg
      481 acctcctgtt catctctgaa gactttttcg acgctcgtta atgacatcaa caaccactgc
      541 gataatcaaa atcaagcctg taaccaggtt ttgccagtag acgtctaccc ccattaaatt
      601 caatgcgttg gcaagcaccg ccatgaacaa agcgcccaaa aatgcgccga acaccgaccc
      661 ctctccgccg ttaaggctag ccccgccaat aatcgtggca gtgatgacgc gcaactctac
      721 accactccca actgtgacgg atgccgagcc aaagcgggcg gtaatcagca agccagcgac
      781 gcccgctaat aaagccacga tacagtaatt gatgatcttg accaaatcaa cgttgatgcc
      841 cgaaaggcgg gcagcttttt catttccgcc aatgtaatag gactggcgga agaaacgcgc
      901 attgcgcatc agcagatctc ctgcgagcac taacgccagc atgacaaaga tggggtactg
      961 cacgctgaac aatcgtcctt gaccgatcac ggtaaaagac tgcgccaggt ttagtactgc
     1021 cctcccctga gccagtacca ataacaagcc gcgaatggtg atcatcattc ccaaggtcgt
     1081 aataaaaggg ttgatcttca tttttgaaac cagaagcccg ttcaccaggc caactcccaa
     1141 ggcagccaac aatccggaga ggatcgccac cggggctggg aagccagcag tcaggacaag
     1201 cccagcgacc acacctgtaa gccctaatgt tgagccaaca gacagatcta aaccgcctga
     1261 gatcaacaga atcaccatac caactgcgat tgtcgcctct acagaaaggc tgagcaggat
     1321 cgcttcaagg ttaggccaag tcagaaaaac aggcgaaacc aagctcatga agatgccaaa
     1381 gatcagcagc accagaatca ggcttaattc acggaaagcg acgagctgcg ctagccaagt
     1441 aggggctttt ctgagttcgg ttgtgcttct ttccattaac acctgctcta tgcgggttta
     1501 gttggataaa aatatataac tcgtcattgc gcctgggcat ttcggttgtc catagatcga
     1561 acaacttgct ccaatccagc ggcaaaagaa aggatcaatg cttcagaaaa atcttcacgc
     1621 cccacctcac ctgtgatgcg cccctgatgc attaccagga tgcggtcgca cattccaatt
     1681 agctcgggca aatccgaaga gatcataatc acgccgacgc ctgttgcagc gaaatcgcgc
     1741 aacctggcat agatctcagc ccgcgcgccg acatccacgc cgcgggtggg ctcgtcgaaa
     1801 atgattactt cgggctggat gcccacccac atggctatca gtgttttctg ctggttcccg
     1861 cccgacaggt tgagcacctt cttttccata gaaggcgtta caatgtcaaa ttgcctgacc
     1921 atctctccgg cgtaacgctc gaggagcggg tgtctgacga aacctaagcg acttgtgaac
     1981 ttattgaacg agggggcaac caggttatcg cgtagtgaca tactcagaaa gagcccctgg
     2041 ccctttcgat cttcggtaag gtaggcgatt ctgtatcgta tagcatcttc cggtttgcgg
     2101 atattcagct cgttaccatt caataggatc cgcccgctat ctctacggtc gataccgaag
     2161 atcgcccgtc ccacttcagt acgctttgca ccaaccaaac cagcgaagcc gaggatctct
     2221 ccacgcctca agctgaagct gacatcctgg aatgagcccc ggcgagagaa gccctccacg
     2281 cgaaaatatt catctcggtc tttgtccgtg cgagcgccat ataagtgggt aatctgccgg
     2341 cctaccatca tggcaactag gttattctcg gtaacatctc tgattggctt gctgtcgaca
     2401 tacttgccat cccgcatgac catcacccgg tccgcaatct ggaatacttc ggagagtttg
     2461 tgggttatgt aaatgaaaga cattccctgc gcctgcaggc ggcggatatt ctggaacaga
     2521 taagcaatct cgctctcagt gagggacgag gtaggctcat cgaggatgag caccttgggg
     2581 tttgttgaga tggccttaag gatctccagg atctgctgct gacccatgga aagttgcttg
     2641 accagccagc gagggttcaa gtccagctcg aaccggtcca gaaagtccgc ggtttgacgg
     2701 tacagctcac cccatcggat aacgttcagc gcgccaaccg gctgccggtt tacaaagata
     2761 ttctcggcta ccgagaggct gcccaccaac gacagctcct ggaaaaccat gctgatgcca
     2821 gatcggatgg catcttcggg tgagttgaac accagcggac ggtcttccag taagatctgg
     2881 ccctcatccg gacgcagggc gccgcccagg atctgcatga gggtgctctt cccggcgccg
     2941 ttctcaccca ccagcgccag gatttcgccc cgcctcaggc tcaggctgac gtggtccacc
     3001 gccagcactc ctgggaagcg cttgcagatg tgttctgccc gtaaaacgtc ttccatcgga
     3061 tccttagagg ggcacagcca gtgttgggac agcgcaggac gcgaagcact cgccgtgccc
     3121 ctaagggagg gttatttcgt atagagcgcg ttggcgtcta agaaatactg caggttgctg
     3181 gcgtcgatat agttgaccga catgaacacg ttggtcgggc cagttgaagc gcccgctttg
     3241 gcgttatcag tagttagcgg cgcctggttg tggacatagt tgtacagggt ttgcagacac
     3301 cagaaggcca tggctgcgtc gtcctgagcc acggtaccgg tcagggtgcc tttctggatc
     3361 ttctccagca cgtcggagtt gcgatccatg gcaacggtct tcacctgacc caccttgcct
     3421 gcctcttcga ccgcagtagc agcaccgatc ccgccggtgg agtccgtgcc gacgaaggct
     3481 gccaggtcag gatagcgctg catgatatct tttgccacac tgacggcggt caccgtgtcg
     3541 gccttggtgt cgccaacctg aacgacctcg atgtcggggt aggcttcgaa ggcagcacga
     3601 aaaccggttt cgcgatcatc gaacatcccc acaccaggga tgctaaggat ggccactttg
     3661 cccttgcccc ccagagcttc ggcgatctta acgccaccgg tgaatcccag gtcgtactgg
     3721 cgcgagccca cgaaggctat acgcttggat tcgggtaggt cacccaggat ggtcaccacc
     3781 gggatgcctg cctcagtggc cttgttgatc gaaggggtga ggacgggctc gacggcgaag
     3841 gtacagatgc ccttgggttt cttggcgatg gcctggtcga aggctgccac catggcgttc
     3901 atatcatatt cagccgggcc tacatatgtg gccgtgacgc ccaggcgctc tccagcccat
     3961 ttccagccgt atttgtgggc gttgaagaac tccaggttgc ccatggcaga gacatagata
     4021 tactcttcgt ccgctgtggc tggctgagcg ctctcgggct gggcttcgct ggccggggca
     4081 gctggctgct gtgcgcaggc tgctgcgagc atagaaatag cgaccagaaa gccaatcagg
     4141 atatacattt tgtttttcat gcgaccttct ccttcttacg ttagactttt tcttagggca
     4201 agaatgccga tgcaccccac ccttttattg cctagctca
//
LOCUS       DBNHOH010000508         2237 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_94202, whole genome shotgun sequence.
ACCESSION   DBNHOH010000508 DBNHOH010000000
VERSION     DBNHOH010000508.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_94202"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="AC16V7_08855"
     CDS             <1..433
                     /locus_tag="AC16V7_08855"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HIR6257269.1"
                     /translation="VYSEGGCLVYPELKEVGDIDNAVINLKFANGTIGNVDVSRNAVY
                     GYDIRTEVLGSEGGITISKTRQTPILIMTRDGVTHDTVPYFMERFADAYAAEIRDFVA
                     CIVEDREPAVTAQDGRAAAAIGIAATRSFDEGRPVRVSEVE"
     gene            683..1816
                     /locus_tag="AC16V7_08860"
     CDS             683..1816
                     /locus_tag="AC16V7_08860"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6257270.1"
                     /translation="MKAIKFALFIIIGALVLAACAPPPEPEVRIVTQEVEVIVTQEVE
                     KEVVVTQEVEKVVEVTPTPIGGQLTLYACLFDPDKLEVIFQTFKAEYGVDVTCLDMSS
                     GEALERIRAEKDNPQGDVLFGTTNLSHVNLAADGLTEPYKGVGWNQLPEGAIKDPDGY
                     WTGFYYGVIGFACSPERLAEIGAECPTSWEDLLDPVYKGEIVIASPAASGTSYTVLSG
                     LVQRLGEDEAFEWYQQFDANVAQYTESGSAPGKMAAAGEFAVGIAFAHDIQVQQDKGL
                     PVVMNFPEEGTPFEIGGISIIKGAKNIAAAQAWVDYVFSPAFQRYHNDVAHRIPVVAG
                     VKLAEGTVGLEDVKLVEGYDPTEWAAQRDALVARWQEEIGSMR"
     gene            1943..>2237
                     /locus_tag="AC16V7_08865"
     CDS             1943..>2237
                     /locus_tag="AC16V7_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257271.1"
                     /translation="MAENIQALPSQMTPKQRRRLWFIGLVIIELLALAYIGTRLVQVE
                     AVVDRTEVVGNVETVYFRNEIVGDPPLLVLLVLGVLLPLVSRRLRRETVLLVAV"
BASE COUNT          547 a          558 c          652 g          480 t
ORIGIN      
        1 ggtgtatagt gagggcggct gcctggtgta cccagagctc aaagaagttg gtgacatcga
       61 caatgcggtc atcaacctca agtttgccaa cggcactatc ggcaacgtcg atgtcagccg
      121 caatgcagtt tatggctacg atatccgcac agaagtactc ggctccgaag gaggcataac
      181 gatcagcaaa accaggcaga cgccgatatt gatcatgacc cgagacggcg ttacacacga
      241 caccgttccc tacttcatgg agcgcttcgc tgacgcctat gcggcagaga tccgtgattt
      301 tgtggcatgc atcgtcgaag atcgtgaacc agccgtgacc gcccaagatg gtcgcgccgc
      361 ggcagccatc ggaattgcag cgacacgttc ctttgatgag ggcagaccgg tgagggttag
      421 cgaagtggaa taagtaccta ggacgtgaat ttccaaagtc atcgttgacg aattgcacaa
      481 aatagattac aaaggttgaa ttgtggtaga acaaaaaccc aacctggaat acacagcctc
      541 cacaaattcg taatcaccag ggaaaattgt atttaagcaa cccgccatga gggaaaggag
      601 gagaatggaa agttagagga gaaaatctac ccccttagaa aatagcatca atacaaatca
      661 caaaaacaag gaggaaaaga gcatgaaggc aatcaaattc gcactgttca tcatcatcgg
      721 cgcattggta ctggccgcgt gtgcgccgcc gccggaaccg gaagttcgca tcgtaaccca
      781 ggaagttgaa gtcatcgtaa cccaggaggt tgaaaaagag gtcgtcgtaa cccaagaggt
      841 ggagaaggtc gttgaagtga cccccacccc gatcggcggc cagctgaccc tttatgcctg
      901 cctgtttgac cccgacaaat tggaagtgat cttccagacc ttcaaagccg agtatggtgt
      961 ggatgtgacc tgcctggata tgagcagcgg cgaggcgctg gaacgtatcc gagcagagaa
     1021 ggacaacccg caaggcgatg tccttttcgg caccaccaac ctgtcccacg tcaacctggc
     1081 ggctgatggg ctgacggagc cgtataaggg cgtgggctgg aaccagttgc ctgagggtgc
     1141 aattaaggat ccggatgggt attggactgg tttttactat ggagtcatcg gctttgcctg
     1201 cagcccggaa cgcctggcgg agattggcgc agaatgcccg acctcctggg aagatttgct
     1261 cgatccagtc tacaagggag agatcgtaat tgccagcccg gcagcctcag gcacttctta
     1321 caccgtcctc agcggtcttg tgcagcgact gggcgaagac gaagcctttg agtggtacca
     1381 gcaatttgac gccaacgtgg cccaatacac cgagtccgga agtgcacccg gtaagatggc
     1441 tgcggcaggc gagtttgcag ttggtatagc ctttgcccat gatatccagg tacagcagga
     1501 taaaggtctg ccagttgtta tgaacttccc tgaggagggc actccgtttg agatcggggg
     1561 catctcgatc atcaaaggtg cgaagaatat tgcggctgct caggcctggg tggactacgt
     1621 cttcagcccc gcctttcagc gctaccacaa cgacgtggct caccgcatac cggtcgttgc
     1681 aggggtgaag ctggccgaag gcacagtagg attggaagac gtcaagcttg tcgaaggcta
     1741 tgatcccacc gagtgggcag cgcagcgcga cgcgttggtc gcccgctggc aggaggagat
     1801 tggctctatg cgctaggttt ttgtgtattc ttttcctggt acaccctaat tttccctccc
     1861 ctttcccttt gagattaaaa aagcgggata ggggagggaa aatctaacaa gtgccgaagg
     1921 aggagcttat tggaggttat gtatggctga gaacatccag gcgctgccaa gtcaaatgac
     1981 cccaaaacaa cgcaggcgtc tatggtttat tgggctggtg atcatcgaat tattagctct
     2041 ggcttacatt ggcacccggt tggtgcaggt tgaggccgtc gtcgatcgta ccgaagtggt
     2101 cggcaacgtg gagactgtct attttcgcaa cgagatcgtt ggcgatccac cgctgctggt
     2161 ccttctggtt ttgggtgtgc tgttacccct agtctctcgg cggctgcgcc gagagacggt
     2221 gctgctggtg gcagtgc
//
LOCUS       DBNHOH010000509         1851 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_735873, whole genome shotgun sequence.
ACCESSION   DBNHOH010000509 DBNHOH010000000
VERSION     DBNHOH010000509.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_735873"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="AC16V7_08870"
     CDS             <1..417
                     /locus_tag="AC16V7_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6257272.1"
                     /translation="KILTSLVGLMGMGAGTLILALAPASAISLAVGGALLVGLMTPIT
                     MGPFLAVIQSTVEPDMQARIFSLLSSVGTGIAPLGLMIAGPVADRVGIQAWFLLGGML
                     CIFMAVTGLFIPAVMNMEKKGNPLPKAVGTSVRQAI"
     gene            449..685
                     /locus_tag="AC16V7_08875"
     CDS             449..685
                     /locus_tag="AC16V7_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257273.1"
                     /translation="MKTTYRSTHYQICLEGILDERWLRWFEGLEVKASADDQTIIRGE
                     FDQSALHGLLNRIFDLGVTLISVQRHPDRKESSH"
     gene            1091..1393
                     /locus_tag="AC16V7_08880"
     CDS             1091..1393
                     /locus_tag="AC16V7_08880"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR6257274.1"
                     /translation="MAEVQITIPKKSLEAFCRRYQIRRLALFGSVLRDDFNRNSDVDV
                     LVVFDPSARVTFMTLGRMKRELSAIFHRPVDLVPQEGLKPAIREAVLADAREVYAT"
     gene            1383..1730
                     /locus_tag="AC16V7_08885"
     CDS             1383..1730
                     /locus_tag="AC16V7_08885"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HIR6257275.1"
                     /translation="MRPEKLYLLDILEASDAVRRFCEAVSEDEFLQDELRQSAVLQKL
                     IVIGEAAARLPTLFQERHADIEWVDIIGFRNIAVHEYFAVLWQIVWATAIQDVPELRA
                     KISKILDQEYQGE"
     gene            1737..>1851
                     /locus_tag="AC16V7_08890"
     CDS             1737..>1851
                     /locus_tag="AC16V7_08890"
                     /inference="COORDINATES: protein motif:HMM:NF046801.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HIR6257276.1"
                     /translation="MRSATAIANPNIAFIKYWGDRERALRLPANGSISMNLD"
BASE COUNT          493 a          426 c          480 g          452 t
ORIGIN      
        1 aagatactca cctcgctggt tgggttgatg gggatgggag ccggtacttt gatcctggcg
       61 ctggcccctg cctcagcgat ttccctggca gtaggtgggg cgctgcttgt cggtctcatg
      121 accccaatta cgatgggacc gttcttagcc gtcatccaat ccacggttga acctgatatg
      181 caagcccgca ttttctctct cctgagcagc gttggcacgg ggatagcacc gctcggattg
      241 atgatcgcag gtcccgtcgc cgatcgggtt gggatccagg cctggttcct gctcggaggc
      301 atgctgtgca tctttatggc ggtgaccggt ttattcatcc ccgccgtgat gaacatggaa
      361 aagaaaggca atcccctgcc caaagcagtt ggcacgagcg tcaggcaagc gatttaacca
      421 aatcagaaga ttaaaagata aagacattat gaaaactacg tatcgatcca ctcattatca
      481 aatctgtctc gagggaattc tcgatgaacg ctggctgcgt tggttcgagg gactggaggt
      541 taaagccagt gctgacgacc aaactatcat ccggggagag ttcgaccagt ctgcgctgca
      601 tggcttgtta aatcgcatct ttgatttggg cgtcacctta atctccgtgc aacgccatcc
      661 agacagaaaa gaatcctcgc attgaaaaaa cctagaaaga acctatttat ggaaacaact
      721 tcctcttagc ttttatcaag aaataagtaa ccacgttcaa aaggtggtta ttacagagga
      781 tcaactttag ttgatagaaa ggttcctgtc ggaagtaagg gaaggaattg aacactcaac
      841 tttcaaagat caaagaaaga tactagacct gcaaaatgtg cgtggtaaac tagccataga
      901 gaacgatgaa aaggttattc acataacgtg cctattaaac ccagaaccgc aacagctgtc
      961 gcttgtgcta acatcgcctt ggctaagtac tcacaaggga caaccgatag taatcgaggt
     1021 gcgcttacac ctgggggaaa tcaggcaatt tcctgtataa ttcctttata tggataagga
     1081 gttatcagac gtggctgaag tgcagatcac catacctaag aagagcctgg aggccttttg
     1141 ccgaagatat cagattcggc gactggcgct atttggctct gtactgcggg acgatttcaa
     1201 ccgaaacagc gacgtggatg tcttagtagt cttcgaccct tccgctcgcg tcacgtttat
     1261 gacgctcggg cgtatgaagc gtgaactatc ggcgatattc caccgcccgg ttgatttggt
     1321 accgcaagag ggtttgaaac cggctatccg tgaagccgtg ctggctgatg ctcgagaggt
     1381 atatgcgacc tgaaaagctt tatctcctag acatcctcga agcctcagac gcggtaagac
     1441 gtttctgcga ggcagtaagc gaggatgaat tcttacaaga tgagctacgt caaagtgctg
     1501 tgctgcaaaa gctaatcgtg atcggagaag cagcagctcg gttgcccacg ctattccagg
     1561 aaaggcatgc tgatattgaa tgggtagata tcatcggctt tagaaatatc gctgtacatg
     1621 agtattttgc tgtgctttgg caaattgtat gggctaccgc tattcaagat gttccggagc
     1681 tacgagcgaa aatatcaaaa atattggatc aggaatacca gggggagtga aaggaaatgc
     1741 gatcagctac tgcaatcgcg aatccaaata ttgcttttat caagtactgg ggcgaccgcg
     1801 agcgtgcctt gcgcttgccg gcgaacggct caatttccat gaacctggac g
//
LOCUS       DBNHOH010000510         5164 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_94444, whole genome shotgun sequence.
ACCESSION   DBNHOH010000510 DBNHOH010000000
VERSION     DBNHOH010000510.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_94444"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="AC16V7_08895"
     CDS             <1..967
                     /locus_tag="AC16V7_08895"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HIR6257277.1"
                     /translation="GCSMEASARAYNVSLMAICETLDLELEEIHDWNCCGATEYLGIS
                     LTPAYALISRNLALAAKQANGSHTVVAPCSACYLNLSKADHFMLERPSLGENVNLALE
                     AGGLHYTPGSLEIRHLLDVFINEIGLDTIKSKVVKPLAGLRVAPYLGCMVPRPDYEHR
                     WSDHEHPNELDNLLKALGAEVIDFPLKTHCCSGHMPQISPSTAFELLRRLIHSAAQYN
                     ADLMVTVCPMCQMNLDAFQHEMNRFYRTNYHMPIVFFTQLIGLAFGIKAAELGFGKEL
                     VDPRPALDRIGVEIPVSEEIKARPKRTKKTGLPMPIMPGDEEVKE"
     gene            964..2997
                     /locus_tag="AC16V7_08900"
     CDS             964..2997
                     /locus_tag="AC16V7_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR6257278.1"
                     /translation="MTVALHPENNGKERIGVYVCHCGSNIAGTVNVEDVSHWASERLH
                     DRGVVIARDYKFMCSSLGQELIENDIQEMGLTRVVVAACSPHLHEQTFRSATARAGLN
                     PYLCELVSIREQVSWVHTDKIAATEKSKAVISGGVERVIHHEPLEPLHVPIHPATLVV
                     GGGIAGIQSALELADAGYPVYMVEREPSIGGHMAQFDKTFPTLDCSACILTPKMVSIG
                     THPNITLLTWSEVTKVEGYVGNFTVTLRKKARYVDTKDCTGCGICQEKCPKKVIDTIY
                     EAGLGYRKAVYTPFPQAVPKYPVVDRANCTYFQKGTCKACEKFCPTGAINFTQEDEFI
                     TVQVGNIVLATGFDLFDARRVSNYGYGRLANVFTSLEFERLSNAAGPTNGNVVLRDGV
                     TVPKAVGIIHCVGSRDRNFNNYCSVICCMQGLKFAHLIHERTGATVYNFYIDMRTAFK
                     AYDEFYQRILEEGTLFVRGKVAEVTDAARLPGEEGKLIIQVEDTLAGKQRRIPVDMVI
                     LSSGLEPRHDSKEVAKLFGISCSADGWFIEKHPKLDPVATMTEGVFIAGCVQGPKDIP
                     ASVSQGAAAAARVLGRIQQGEIALEPVRATVNENKCSGCRICNDLCPFNAILFHEDRM
                     VTEVNAALCQGCGTCIAACPAGAIAGTGFSNEQILSQIEGLLLLNLAPELVPA"
     gene            3063..3536
                     /locus_tag="AC16V7_08905"
     CDS             3063..3536
                     /locus_tag="AC16V7_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HIR6257279.1"
                     /translation="MNDNHIFEPVIIGFTCNWCSYRAADLAGTARMKYPPNVRLIRLM
                     CSGRLDPTFVIKAFSGGADGVMITGCHPGECHYIEQNYKALRRYLLLRRTLAQFGIEP
                     ERIKLVWASAAEGARLSEEITKMVEDIRTLGPLNWPKLSNGRPDHTVLLQEEVKV"
     gene            3533..4537
                     /locus_tag="AC16V7_08910"
     CDS             3533..4537
                     /locus_tag="AC16V7_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HIR6257280.1"
                     /translation="MTTNGKPKFAMYWAASCGGCEIAVLNIHEKILDVDANFEVAFWP
                     VAMDAKYKDVQAMPDKSILLTLFNGGIRNNEHEEIAHLMRRKSQILVAFGSCACEGCI
                     PGLANFSQTEQIFEAAYQSITTDNPDRILPQYDYTMPEGEIHIPEFYPVLKTLDQVVP
                     VDYYMPGCPPESHQIAGVIDLVIKVLNGEATLPPPGSVLGAGDSTVCDDCKRERNVKT
                     IKKFIRIQELDHLDPALCLLEQGVPCNGPATRQGCGALCPTAGAQCIGCYGPVEGVID
                     YGARLIAAFASVIDSNDPLEIDRILDGIPDPAGQFYRFNLAGSLLQASRVAWKSNGSV
                     "
     gene            4547..>5164
                     /locus_tag="AC16V7_08915"
     CDS             4547..>5164
                     /locus_tag="AC16V7_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HIR6257281.1"
                     /translation="MTQRITIDPITRLEGHGKIEIFLNEDGDVANTYFQIPELRGFER
                     FCVGRPVEEMPLLTNRICGVCPEAHHMAAAKAADAVYHVDPPRTAKMLRELLYSAFYV
                     TDHTTHFYALAGPDFVMGPESPVAQRNILGVIHKVGLEVAGKVIQARKYGHTVIEMIG
                     GRKVHPCTSIPGGLTKGITEEQRDEIEKMGRWAIEFAQFTLQLFND"
BASE COUNT         1308 a         1300 c         1303 g         1253 t
ORIGIN      
        1 cggttgctcc atggaagcca gtgctagagc ctataacgtt tctttaatgg caatttgcga
       61 gacacttgac cttgagttgg aagaaattca tgattggaac tgctgtggcg ctaccgagta
      121 cctgggaata agcctgactc ctgcgtatgc gctgattagc cgcaacttgg cattggctgc
      181 caagcaagcg aatggctcac acacagttgt cgcaccgtgc agtgcttgct atctcaacct
      241 atcaaaggca gatcacttta tgctcgaacg gccatcactc ggtgagaacg tcaaccttgc
      301 cttggaggcc ggcggattac attatacgcc tggatcgttg gaaatccgcc atttactgga
      361 tgtctttatc aatgaaatag gcttggatac gataaaaagc aaagtagtca aaccactcgc
      421 tggacttcga gtcgctccct atctgggatg catggtacct cgccctgatt atgaacatcg
      481 ttggtcggat catgagcacc cgaatgaact ggataacctt ctaaaagcac tgggtgcaga
      541 agtgatcgac ttcccattaa agacacattg ctgcagcgga cacatgcctc aaatcagccc
      601 atctacggct ttcgaactgc tgagaaggtt aattcactca gctgctcaat ataacgcaga
      661 cttgatggtg accgtctgcc caatgtgcca aatgaacctg gatgcattcc aacacgagat
      721 gaaccgtttc taccgtacga actaccatat gccaattgta ttcttcaccc agctcatcgg
      781 tttagcattc ggaataaaag ccgccgaact tggctttggt aaagagctgg ttgatcctcg
      841 gccagcattg gaccgcatag gagtagaaat ccctgtttcc gaagaaatca aagctcggcc
      901 taagcgaaca aagaagaccg gacttcccat gcctattatg ccaggcgatg aagaggtgaa
      961 agaatgacag tagcattaca tcccgaaaat aacggcaaag agcgcattgg tgtctatgta
     1021 tgccactgcg gcagtaatat cgccggcaca gtaaacgtag aggatgtgtc gcattgggca
     1081 agtgagagat tgcatgatcg cggggtcgtc atcgctcgcg attacaaatt tatgtgctca
     1141 agcctggggc aagaacttat tgaaaacgac atccaggaga tgggactgac ccgtgtcgtc
     1201 gtagctgcct gctcgccaca ccttcacgaa cagacttttc gttccgccac cgcccgggct
     1261 ggcttgaacc cgtatctgtg tgagttggtt tctatacgcg aacaggtctc ctgggtacat
     1321 accgacaaaa tcgctgcaac ggagaagtcg aaagcagtaa tctcaggcgg cgttgaacgc
     1381 gtgatacacc acgagccttt ggagccattg catgtcccga tacatcccgc tacgctggta
     1441 gttgggggcg gaatcgctgg aattcaatca gccctggagc ttgctgatgc cgggtatcca
     1501 gtctacatgg tcgaacgtga gccatccatt ggcggtcaca tggctcagtt tgataaaacc
     1561 tttcccacgc tagattgctc tgcttgcatc ctgaccccaa agatggtatc gattggaaca
     1621 catccgaata tcactctgct cacctggagt gaggtgacca aggtagaagg atatgttggc
     1681 aacttcacag tcactcttcg taagaaagct cgctacgtag acactaaaga ctgcactgga
     1741 tgcggtattt gtcaagaaaa atgcccaaag aaggttatcg acacaatcta cgaggctgga
     1801 ttgggttacc gcaaagcggt ctatacgcct ttcccacaag cagttccgaa atatcctgtt
     1861 gtggatcgag caaactgcac atacttccag aaaggcactt gcaaagcttg tgaaaaattc
     1921 tgccccactg gtgcgatcaa ctttactcaa gaggatgaat tcatcaccgt tcaagtcgga
     1981 aatatcgtgc ttgcaaccgg ctttgacctg ttcgatgctc ggagagtctc taattatggt
     2041 tatggacgac tcgctaacgt ttttacaagt ctggagttcg aacgcttgtc taacgctgct
     2101 ggccccacta acgggaatgt ggttctccgg gatggtgtaa ctgtccccaa agctgttggt
     2161 attatccact gcgtgggcag tcgcgaccgt aacttcaaca actattgctc ggtcatttgt
     2221 tgtatgcaag ggttgaaatt cgctcatctg atccacgagc gaaccggagc aacggtttac
     2281 aacttctata ttgatatgcg taccgccttc aaagcttacg acgagtttta ccagcggata
     2341 ctcgaagaag gaacactctt tgtgcgcggt aaggtcgccg aggtgacgga tgcagctcgc
     2401 ttacccggcg aagaaggcaa actgatcatc caggttgagg acactctcgc tggtaagcag
     2461 cgacgtattc cggtagatat ggtcatcctc tcatctggac ttgagccacg ccacgactca
     2521 aaggaagtcg ccaagctgtt tggtatctcc tgcagcgctg atggctggtt tatcgaaaag
     2581 caccccaaac ttgacccggt cgcaaccatg acagaaggcg tatttatcgc tggttgcgtg
     2641 caaggtccaa aggatatccc tgccagcgtc tcccaaggag cagcggcggc ggcgcgcgtc
     2701 ctgggtcgta tccaacaagg cgaaattgct ctcgaacctg tacgtgctac cgttaacgaa
     2761 aacaagtgct ctggctgccg gatttgcaac gatctgtgcc cgttcaatgc gatccttttc
     2821 cacgaagatc gtatggtgac tgaagtcaat gctgctctct gccagggatg cgggacttgc
     2881 atagccgctt gccctgctgg agctatcgct ggaactggat tcagtaatga acagattctc
     2941 tcccagatcg agggtttgct gctgctcaat ttagcgccag aactcgttcc tgcttgaagg
     3001 gcgaaccatg aagcaattca acccaatcca acaaagcgtc atcttccaat gggagatcaa
     3061 ccatgaatga taatcatatt tttgaacccg tgataattgg gtttacctgt aactggtgct
     3121 cctatcgcgc cgcagacttg gctggcactg cacgcatgaa atatccacca aatgtccgtc
     3181 tgattcgtct gatgtgctca ggtcggttag atccaacttt tgtgataaaa gcgttctcgg
     3241 gcggagccga tggggtcatg attacgggtt gccatcctgg tgaatgtcat tacatcgaac
     3301 agaactacaa agctttacgg cgttacttac tcttacgccg cacccttgct caatttggca
     3361 ttgaaccgga gcgcattaaa cttgtatggg caagtgcagc tgagggggct aggctatccg
     3421 aggaaatcac caaaatggta gaggatattc gcactcttgg tccactgaac tggccaaaac
     3481 tgtccaacgg acgccctgac cacaccgtgt tgctgcagga ggaggtaaaa gtatgactac
     3541 aaatggcaag ccgaaattcg cgatgtactg ggctgcctcc tgcgggggtt gcgagatcgc
     3601 tgtgctcaat atccacgaga agattttgga tgtagatgct aacttcgagg tcgcgttctg
     3661 gcctgtagcc atggatgcca agtacaaaga tgtgcaggcc atgcccgata agtccatttt
     3721 gctcactcta ttcaatggtg gcattcgcaa caacgaacac gaggagattg ctcacttgat
     3781 gagacgcaag tcgcagatcc ttgtcgcctt cggatcctgc gcatgtgaag gttgcatacc
     3841 gggtttggcg aactttagcc aaacggagca aatatttgag gcagcatacc agagcattac
     3901 aaccgataac ccagacagaa tcctgcctca atacgactat acaatgcctg aaggcgagat
     3961 tcacatccca gagttctatc cggtgctaaa aacactcgat caggtggtgc ccgtggatta
     4021 ctacatgccg ggttgcccac ccgagtctca ccagattgca ggtgtaatcg atctcgtcat
     4081 caaggtatta aatggtgagg ccaccctacc gcccccaggt tcagtattag gagcgggtga
     4141 ttccacagta tgcgatgact gcaaacggga acgcaatgta aaaacgatca agaagtttat
     4201 ccgcatccag gaattagacc accttgatcc tgccctttgc ttgttagagc agggtgtccc
     4261 atgtaacggt cctgccaccc gccaaggatg tggtgcttta tgtcctactg ctggcgccca
     4321 gtgtattggc tgctatggtc ccgtagaagg tgtcatcgat tatggcgccc gtcttattgc
     4381 ggcctttgca tcagtcattg acagtaatga cccgctagaa attgatcgta ttctggatgg
     4441 gatacccgat ccagccgggc aattctacag gttcaattta gccggctcat tgttgcaggc
     4501 cagtagagtc gcatggaaaa gcaacggctc cgtataggag gatcgcatga cgcaaagaat
     4561 tacgattgac cccatcaccc gcctggaagg tcacggcaag atagaaattt ttttgaatga
     4621 agacggtgac gtcgctaaca cttatttcca aattcccgaa ctgcgtggtt tcgagcgctt
     4681 ctgtgttggt cgcccggtag aggaaatgcc cctactcact aatcgcatct gtggtgtatg
     4741 tccagaagcg caccacatgg cagcagctaa agctgccgat gctgtctatc acgttgatcc
     4801 tccgcgaacc gcaaagatgc ttcgagagct gctgtacagt gctttttacg tcacggatca
     4861 cactacacat ttttacgccc tggctggtcc agacttcgta atgggtccgg agtcccctgt
     4921 tgctcaacgt aacatactgg gagtgatcca taaggttgga ctggaggtag ctggtaaggt
     4981 tatccaagcg cgcaagtacg gtcacaccgt aattgaaatg atcggcgggc gcaaagtgca
     5041 tccttgcacc tcaattccag gtggattaac caagggtatt accgaagaac aacgcgacga
     5101 gattgagaaa atgggccgtt gggcaatcga gtttgcccag tttaccctcc agttattcaa
     5161 cgat
//
LOCUS       DBNHOH010000511         5686 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_286964, whole genome shotgun sequence.
ACCESSION   DBNHOH010000511 DBNHOH010000000
VERSION     DBNHOH010000511.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5686)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_286964"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..880)
                     /gene="surE"
                     /locus_tag="AC16V7_08920"
     CDS             complement(113..880)
                     /gene="surE"
                     /locus_tag="AC16V7_08920"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HIR6257282.1"
                     /translation="MHILVTNDDGVTASGLLALAQEMRQVGQVTVLAPDRNWSASGHV
                     KTLERPLRVRDVILADGTPALATDGAPSDCVALAVLGLLPSKIDLVVSGINPYANLGH
                     DVTYSGTVTAAMEAAIWGLPGIAVSLNSTENDRGPLDYGPAARVARRVVAEVIARQLP
                     KATLLNVNVPYLPDEQLKGIRITRQGLRVYHDLLVTREDPRGRPYYWIGGDAPTGVPE
                     NGTDFGALSEGFVSITPLHLDLTAYRALEEMSEWKWE"
     gene            1008..1814
                     /locus_tag="AC16V7_08925"
     CDS             1008..1814
                     /locus_tag="AC16V7_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257283.1"
                     /translation="MTTSKRLTGLLMVIVAAGSLMITLAALVQVWRLRQPVTQYLRSN
                     LRVVHESLGTTSDGLLLTSQSLDSVQASVTALSVTVDTLGKSIQDSEPMMDSLVVLMG
                     EDLPETISSAQTSLSSAQESAKIIDAVLRALTIFNPRIYNPRVPLNDALGQVSESLDD
                     LPKSFSNMEVGLKSTQRNLTTIQVQIDLMSGEIERINESLDGSRKVINQYQGLFTRAE
                     KRVINIKNRAPATVTTLAWVLSFLLFWLGVAQVGLLIRGLEWLRVKKQPD"
     gene            complement(1851..2693)
                     /locus_tag="AC16V7_08930"
     CDS             complement(1851..2693)
                     /locus_tag="AC16V7_08930"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6257284.1"
                     /translation="MPVFHRMRSSSLFLFVALVALLSWNFVHAQTSGEEQMEQGASIY
                     AANCAVCHGDDGQGRVGATLAKDWPSIRPDLKVRATIENGIAGSPMPAWSQKNGGPLT
                     DGEIDALVVYILSWETGGPRVFPPTPTYIPRPLISPVPDVVGDPNQGAILYDQNCAVC
                     HGTEGRGRVGASLDKVWPSIRPDLGIKNTISNGVMGSPMPAWSQVNGGPLTETEINNL
                     VAFIMALEPASEEIQQAPTPAPAPGPLADWFGILLAVVLFILVVWIAIVLQTRTQAQN
                     DTEK"
     gene            complement(2701..3546)
                     /locus_tag="AC16V7_08935"
     CDS             complement(2701..3546)
                     /locus_tag="AC16V7_08935"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6257285.1"
                     /translation="MSRVQLEISLGILLVLATGTILIYQGLLEEERMARFEEQQRAQA
                     IEVGAELFDINCKGCHGPQGEGTPGLCPPLNDKYFFTDRLKDVGWSGSIEDYIVATVA
                     SGRLTSTRPELYPGNVRPAMPAWSEQYGGPLREDQIRFIAAFVANWEATAPDRQLAPV
                     LAGPPVGTDITQELPAGDAVKGEALASSQGCIGCHVAGPTGPPWNPTADQPGIGDRAA
                     ARLTQSDYSGNAATPEQYLLESIVNPAAYLVPGYETVLMPGTYGQTITAQDASDLIAY
                     LLTVK"
     gene            complement(3577..5247)
                     /locus_tag="AC16V7_08940"
     CDS             complement(3577..5247)
                     /locus_tag="AC16V7_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012263.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008121 - quinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HIR6257286.1"
                     /translation="MLKSLNFQDLRKKALEAFDNRVRIITAGMGLDELRAVVRGDPPT
                     EKPNPRYKVHTTSFLFHIRPRYYEKGSTIFAHTFRLGFFTSFFFFVELFTGLILMIYY
                     TPSPAEAYNSILELMSNVPFGQVLRDLHRIGAEGMVIFSVLHMLRTFLTGSYKKDRTF
                     TWLTGVFLLGITLFLSFSGYLLPWDQLSYWAVTIGTSMAEAAPLFGTQVNLLLRGAPD
                     IGAGGLLRFYLFHVVLFPLLAIWVISIHYYKVSREHGISLPANVEEGNVAPEAKKEAK
                     QRIDYIPDLLTHEVFLTALGLFVLVALVTVFNWHAPLEHVANPQQTPLDTKAPWYFWW
                     LQGMLKIDPASILENLFSRVGLQVELSALLNSKQIMGIVLPTILAGLLLGVPYIDRNP
                     HRSLYKRPVAVGFGLLFTLVLVVLSYMGLPEYGIQTPAATRIIQDLAPEEGIGQLREV
                     PFDQLRVGVYEVGDTIPRNLCPDEPAGCPELTGVFAEFSERINSAAKNEHLEPYQRLP
                     NAQAVMLIEDWQHDLRKVTLRILWDDVASGQPKSYERHVFLHRAHGAE"
     gene            complement(5292..>5686)
                     /locus_tag="AC16V7_08945"
     CDS             complement(5292..>5686)
                     /locus_tag="AC16V7_08945"
                     /EC_number="7.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="QcrA and Rieske domain-containing protein"
                     /protein_id="HIR6257287.1"
                     /translation="LFSVGRVSELPEAGASPVNFPKVKLWLSNTEEGVMALYKVCTHL
                     GCLYNWNSQEGKFICPCHGSQFEANGEYIQGPAPRSLDRFVVQAVDPNTNQVLAEAAD
                     GKPMPVPDQSDAVIRVDTGKRIPGDVHA"
BASE COUNT         1388 a         1562 c         1534 g         1202 t
ORIGIN      
        1 taatattagc caaagatatg cctccaacat cgaactaaag tacggcgtgt tattttacat
       61 gaggatggcg gatttgtcca gttctgaact cgatttgccg gtattcagaa ggtcactccc
      121 acttccactc gctcatctct tccagcgccc ggtaggcggt caggtccaga tgcagtgggg
      181 tgatcgagac aaacccctct gacagtgcac cgaaatcagt cccattttcc gggacaccgg
      241 tgggggcatc gccgccgatc cagtaatacg ggcgaccgcg cgggtcttcg cgggtcacca
      301 gcagatcatg gtatacgcgc agcccctgcc gggtgatccg tatccccttc aactgttcat
      361 caggcaggta agggacattt acatttaata aagtagcttt aggcaattgg cgagcgatta
      421 cctcagccac caccctacgg gcaacgcgcg ccgcaggacc gtaatccagc ggaccgcgat
      481 cattctccgt ggaatttaat gaaacggcaa ttccaggaag cccccagatg gctgcttcca
      541 tggcggcagt gaccgttccc gagtaggtca catcgtgacc caggttggca taggggttta
      601 taccagacac aaccagatcg atcttgctgg gcaacaagcc caacactgcc agtgcaacgc
      661 aatccgatgg tgcgccatcg gtggctagtg ccggggtacc atcggctaag atgacatctc
      721 taacccgtaa gggacgttcc agcgttttga catgcccaga ggctgaccag ttacggtctg
      781 gtgccagcac ggtcacctga cccacctggc gcatctcctg cgccaaggca agtaagcctg
      841 aggcagttac accatcatcg tttgtgacaa gaatgtgcat aggtttttat ctatcggtag
      901 gtgaaactca taggatggag atgaattata aatcaaatga cacaagatga aagattgaga
      961 tggtaaaatg ggttctgatc cttgcatact agccataagg gaaaaagatg acaacctcta
     1021 agcgcctcac tggtctgctg atggttattg tggcagccgg tagcctcatg atcaccttgg
     1081 cggctttggt ccaggtttgg cgactgcgcc agccggtgac gcaatatctt cgatccaacc
     1141 tgcgagtggt ccacgaaagc ctggggacca cctcggatgg cctgctcctc accagccagt
     1201 cgctagattc cgtacaggcc agtgtaactg ccttgagtgt caccgtggac accttgggca
     1261 aatccatcca ggattcagag cccatgatgg attcgctggt ggtcttgatg ggcgaggact
     1321 tgccagaaac aatctcctcc gcccagacat ctttgtcctc cgcacaagag agcgcaaaaa
     1381 ttattgacgc agtcctgcgt gcattgacga ttttcaaccc caggatctat aacccacgcg
     1441 ttccgctgaa tgacgccttg ggccaggttt ctgagagcct ggatgacctg ccaaaatctt
     1501 tttccaatat ggaggtgggg ctgaaaagca cgcagcgtaa cctgacaacc attcaggtgc
     1561 aaatcgactt gatgtctggg gaaatcgagc gcatcaacga aagcctggat ggatcgagaa
     1621 aagtgatcaa ccaataccag gggctgttca ccagagcgga aaagagggtt atcaacataa
     1681 aaaaccgcgc tccagccaca gtcaccacat tggcctgggt gttaagcttc ttactctttt
     1741 ggctaggtgt cgctcaagta ggattactaa ttcgcggact ggaatggctg agagttaaaa
     1801 agcaacctga ttaatgatga ttgatatggg ggcaagggcc cccatatcaa tcatttctct
     1861 gtatcgtttt gagcctgagt gcgagtttgc aggacaattg cgatccaaac taccaagata
     1921 aataagacca ccgccagcaa tataccgaac caatcggcca gcggccctgg cgcgggtgcc
     1981 ggtgtaggag cctgctggat ttcctcgctt gcaggttcca acgccataat aaacgccacc
     2041 aggttattga tttcggtctc ggtgagcgga ccgccattga cctggctcca ggcgggcatc
     2101 ggcgagccca tcaccccatt cgaaatggta ttcttgatgc ccagatcggg acggattgag
     2161 ggccagactt tgtccaggct ggcgccaacg cgcccccgtc cttcggttcc atggcaaacc
     2221 gcgcaattct ggtcgtacag gattgccccc tgattaggat cgccgaccac atccggtacc
     2281 gggctgatga gcgggcgagg gatataggta ggagtaggcg ggaagacccg cgggccacct
     2341 gtctcccagc tcaagatgta caccaccaag gcgtcaattt ccccgtctgt gagcgggcca
     2401 ccatttttct ggctccaagc cggcatgggc gagccagcaa taccattctc aatcgttgcg
     2461 cgcactttta agtcgggtcg gatggaaggc cagtccttcg ccagggtagc acccacgcgc
     2521 ccttgaccat catcgccatg gcaaactgcg caattcgcag catagatgct ggcgccctgc
     2581 tccatttgct cctcgcctga tgtctgagcg tgcacaaaat tccagctcag caaagcaacc
     2641 agagcaacaa agagaaaaag gctagatgag cgcatgcggt gaaagaccgg catattctcc
     2701 tcacttcacg gtaagcaggt aagcgatcag atcagacgca tcttgagcgg taatcgtctg
     2761 tccgtaagta ccaggcatca gaacagtttc gtaaccgggc actaggtaag ccgcaggatt
     2821 cacgatcgac tccaacaggt attgttcagg cgtagccgca ttgccagagt aatcactctg
     2881 cgtcaggcgt gcggctgcac ggtcgccaat tccgggctga tcggcagttg ggttccaggg
     2941 cgggccggta ggcccagcca cgtgacagcc aatgcagccc tgcgaggagg ccagggcttc
     3001 gcctttcacc gcatctccgg caggtaattc ctgggtgatg tcagtaccga cgggcggtcc
     3061 ggccagaaca ggggccaact gccggtcggg ggcggtggct tcccaattgg caacaaaagc
     3121 agcgataaag cggatctgat cctcacgcag cggtccgcca tattgctcgg accaggcagg
     3181 catggctggg cgcacattac cagggtacag ctcgggacgc gtggaggtca gccgtccact
     3241 ggctacagtt gccacaatgt aatcctctat ggaaccgctc cagccgacgt ctttaagtcg
     3301 gtcggtaaag aaatacttat cgttgagcgg cgggcagagg cctggggtgc cttcgccttg
     3361 tgggccatga caacccttgc agttgatatc aaatagctct gccccgacct caatcgcctg
     3421 ggcgcgctgc tgttcttcga agcgcgccat gcgctcttct tctagtaatc cctggtaaat
     3481 caggatcgtg ccggttgcca gcaccagcag gatgcccaat gagatctcaa gttgcactcg
     3541 tgacataatg atgagaaacc ctcctatagc cagatgctat tccgctccgt gagcacggtg
     3601 caggaagaca tggcgttcat aactctttgg ctggcccgaa gcgacgtcat cccataaaat
     3661 acgcagggtg accttgcgca ggtcgtgctg ccaatcttcg atcaacatca ccgcttgggc
     3721 gttgggcagt cgctggtaag gctcaagatg ctcattcttc gccgctgaat ttatccgctc
     3781 gctgaactcg gcgaacacgc cagtcagctc agggcaaccc gcgggttcat ccggacaaag
     3841 gttcctcggg atagtatcac ccacctcgta cacacccacg cgcaattgat caaacggcac
     3901 ttcgcgcaat tggcctatac cctcttcagg tgccagatcc tggatgatgc gcgtggcggc
     3961 gggcgtttga attccatact caggcagacc catatagctg agcacaacaa gcaccagggt
     4021 aaatagcagg ccaaacccaa ccgccaccgg tcttttgtac aaactgcgat gcgggtttcg
     4081 gtcaatgtaa ggcacaccca gaagcaaccc tgcaagaatg gtaggcaaga ctatccccat
     4141 gatctgcttg ctgttcaata aggcactcaa ttcaacctgc aagcctactc tgctgaacag
     4201 gttttcgaga atgcttgccg ggtcaatttt aagcatccct tgcaaccacc agaagtacca
     4261 cggggctttc gtgtccaggg gggtttgctg cgggttcgcc acgtgttcca gaggagcatg
     4321 ccaattgaag accgtgacca aggcaaccaa tacgaacaac cctagagcag tgaggaagac
     4381 ttcatgggtg agcaagtcag ggatatagtc aatccgctgc ttggcttctt tcttagcttc
     4441 cggcgcgaca ttgccttctt caacatttgc cggcagggag ataccatgtt cgcgtgatac
     4501 tttatagtaa tggatgctga tcacccagat agccagcagc gggaagagca cgacatggaa
     4561 gaggtaaaaa cgcaacaaac cgcccgcgcc aatatccggc gcgccgcgca gcagcaggtt
     4621 gacctgggtg ccgaacaatg gggccgcctc agccatgctg gtaccgatcg tcaccgccca
     4681 gtaggagagc tgatcccagg gcaacagata gccgctaaag ctgagaaata aggtaatacc
     4741 cagcaggaat acgccggtaa gccaggtgaa cgtgcggtct tttttgtatg agccggtcaa
     4801 gaaggtacgc agcatgtgca acacgctgaa gatgaccatc ccctccgcac caatacgatg
     4861 cagatcgcgc agcacctgtc caaaggggac attgctcatc aattctagaa tcgagttgta
     4921 agcctcagcc ggagatgggg tgtaatagat catcaggatc agaccggtga acagctcgac
     4981 gaagaaaaag aagctagtga aaaatcctag ccgaaaggtg tgagcaaata tcgtgctgcc
     5041 cttttcataa tagcgcgggc gaatatgaaa gaggaagctg gtggtgtgaa ctttatagcg
     5101 gggatttggc ttctcggtag ggggatcacc gcgtaccaca gcgcgcagtt catccaaccc
     5161 catgccggcg gtgataatgc gcacccgatt gtcgaaagcc tcgagggctt ttttacgtaa
     5221 atcctggaaa ttgagcgatt tcagcataga accttctcag taactagaac ctaaagtgag
     5281 ttgggatgct tttaggcgtg gacatcaccc gggatacgct tgcctgtatc cactcgaatg
     5341 acagcgtcag actggtcagg aacaggcata ggcttgccat cagccgcctc ggcgagcacc
     5401 tggttagtgt tgggatcaac cgcctggacg acaaaacggt ccagcgaacg aggggccggt
     5461 ccttgaatat attcaccatt agcctcaaac tgagagccat ggcacgggca gataaattta
     5521 ccctcttggg agttccagtt gtacaggcaa cccaggtgtg tacaaacctt ataaagtgcc
     5581 atgacaccct cttcggtgtt gctaagccac agcttgacct tggggaagtt aacaggagac
     5641 gcccctgcct ctgggagctc ggaaaccctg cccacactaa agagac
//
LOCUS       DBNHOH010000512         2485 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_671783, whole genome shotgun sequence.
ACCESSION   DBNHOH010000512 DBNHOH010000000
VERSION     DBNHOH010000512.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_671783"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1291)
                     /locus_tag="AC16V7_08950"
     CDS             complement(<1..1291)
                     /locus_tag="AC16V7_08950"
                     /inference="COORDINATES: protein motif:HMM:NF024867.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR6257288.1"
                     /translation="MLKELHIENFAIIDKIDLQLGAGLITFTGETGAGKSILIDAVET
                     LLGGRADATMVRSGCERAYVEGRFYISPPVREPVHSILKREELLDDPEYVTLGREIRL
                     NERNIARLNGRSVNATLLREIGEYLVDVHGQSEHLSLLRVRQHLGLLDNYAGLEDLLG
                     GYQKTYHKLQAVRSELGELRQAEQEAARRIDTLNYQINEIEAAHFRPGEEETLRDERN
                     RLANAEGLASLANEALQALDEGTSETPAVTDLFGQAVHALNNLAKLDPSQDNLSNQGQ
                     LLFDSLSEMVREIRSYLEGIEFNPKRLDQVEERLGLLQNLKRKYGESIEAILQYAERA
                     RQQLDAITHASERIQELEFDEAKLLSQIGIQGSMLSEKRREQAEKLGRSLENELYDLQ
                     MSGTRFEVEFRSRPDPNGAPLRDGQRVAFDSSGLEQAE"
     gene            complement(1310..1831)
                     /locus_tag="AC16V7_08955"
     CDS             complement(1310..1831)
                     /locus_tag="AC16V7_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B-box zinc finger protein"
                     /protein_id="HIR6257289.1"
                     /translation="MTDIPATLYCENHPQTETNLRCNRCSKPICPKCAISTPTGYRCA
                     ECVRNQQKLFETSRWYDYVSATILAGLLSLVGSRIITSFGFFTILLAPVAGVIIAEVV
                     RFAIQRRRSKLLFQIAAIATAVGSSPFLLIGLLILLQGNLGGLWSVLWQGVYTFIVTS
                     TVYYRLGGIQMRV"
     gene            complement(1828..>2485)
                     /locus_tag="AC16V7_08960"
     CDS             complement(1828..>2485)
                     /locus_tag="AC16V7_08960"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039482.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD+ metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR6257290.1"
                     /translation="RAGHLCGPLGLPILGINLGHFGFLMEVRQNQWMDILPRLLTGDY
                     WLEQRMMLNAELYRSDEILGTWDVLNEVVVGRGQTVRPVQLVAEVDGRYLTTYVADAL
                     IAATPTGSTAYALAAGGPILPPELRNILLVAVAPHLSIDRAIVLAEGSSVGITIHSTH
                     HAVLSVDGQTPINVQDGDKVIARASDYTLKFVRFQDPGYFYRNLTPHMEQNPTTGNQR
                     "
BASE COUNT          547 a          668 c          675 g          595 t
ORIGIN      
        1 attctgcctg ctccagcccg ctcgagtcga acgctactcg ttgtccatca cggaggggtg
       61 cgccgtttgg gtcgggtcgg ctccgaaatt ccacctcgaa cctagtcccc gacatttgca
      121 ggtcgtagag ctcattttcc agggaccgcc ccagcttctc agcttgctcc cgacgctttt
      181 cagagagcat acttccctgt atgccaattt gggaaagcaa tttggcctca tcaaattcaa
      241 gctcctggat gcgctcgctt gcatgggtaa tggcgtctaa ctgctggcga gccctctcag
      301 cgtattgaag tattgcttcg atgctctcgc catatttgcg ttttaagttt tggagcaacc
      361 caaggcgctc ttccacctgg tcgagccgct ttgggttgaa ttcaatacct tctaagtaag
      421 agcgaatttc cctgaccatc tctgagaggc tatcgaataa gagctgacct tgattgctca
      481 gattatcttg tgatggatcc agcttggcaa gattattcaa ggcgtggaca gcttgcccaa
      541 acaggtcggt aacggctggt gtttcagatg tgccttcatc taaagcttgc aacgcctcat
      601 ttgcgagtga agccagccct tcggcattcg ctaggcgatt gcgctcatct cgcagtgtct
      661 cttcttcgcc tgggcggaaa tgggctgctt cgatttcgtt gatctggtag ttcagcgtgt
      721 cgatgcggcg ggcggcttcc tgttcagctt ggcgtagctc gcccagctcg ctccgcacag
      781 cttgcagctt gtggtaggtc ttctggtaac ctcccagcag gtcttccaag cccgcgtaat
      841 tatccagcag tccgaggtgc tggcggacac gcagcaagga taagtgctca gactgtccat
      901 gaacatcgac cagatactcg ccgatctctc gcagtagtgt ggcgttgacg ctgcgcccat
      961 tgagacgggc aatgttgcgc tcgttgaggc gaatttcccg cccgagggtc acatattctg
     1021 ggtcgtcaag cagctcttcg cgctttaaaa tagaatgtac tggctcacgc acaggggggg
     1081 aaatatagaa tctgccttct acataggcgc gctcgcaacc gctgcggacc attgtggcat
     1141 ctgctcgacc accaagcaag gtttccacgg catcgatcag gatggatttg cctgcgccag
     1201 tctcgccagt gaaagtgatc agacctgcgc caagctgcag atcgatctta tcaataatcg
     1261 cgaagttctc tatgtgtaat tctttgagca tactcgcgca acagccccat cagacgcgca
     1321 tttggatgcc gcctaaacgg tagtatacgg tcgatgtcac aataaaggtg tagactcctt
     1381 gccaaaggac actccatagt cctcccaggt tgccttgtaa taagatcaag aggccgatca
     1441 gcaggaatgg tgagcttccc accgctgtag cgattgccgc gatctgaaaa agaagcttgg
     1501 aacggcggcg ctgtatagca aagcgaacca cctcggcgat aatcactcca gcgactgggg
     1561 ccagcaaaat cgtgaaaaac ccaaaactcg tgataatgcg gctacctacc agggaaagca
     1621 gacctgctaa gatcgtagca gacacatagt cataccagcg gctggtttcg aacaactttt
     1681 gctggtttcg cacgcactct gcgcagcgat agccagtggg tgtggagata gcacactttg
     1741 gacagatcgg tttgctgcaa cgattgcagc gaaggttggt ctcggtttgg gggtgatttt
     1801 cacaataaag ggtggcgggg atatcagtca tcgttgatta cctgtcgttg ggttttgctc
     1861 catgtgaggg gttagatttc gataaaaata gcctggatct tggaagcgaa cgaattttaa
     1921 cgtatagtca ctggcgcggg cgatgacctt gtcgccgtct tgcacgttga tcggcgtttg
     1981 tccatcaacc gaaaggaccg cgtggtgcgt gctgtgtatc gtgataccaa cagacgatcc
     2041 ttctgccagg acaatcgctc gatcaatcga taggtgcggt gcaacagcga ccagtaatat
     2101 gttgcgcaac tcggggggca agatggggcc gcccgctgcc agcgcgtacg cggtggagcc
     2161 agtgggagta gcagcgatca gggcatcggc gacatacgtg gtcaggtagc gcccgtctac
     2221 ctctgccacg agctgcactg gccttacggt ctggcctcgt ccaaccacga cttcgttgag
     2281 cacgtcccat gtgccgagga tttcatcgct gcgatatagc tcggcattca gcatcatgcg
     2341 ttgctctaac cagtaatcgc cggtcaataa gcggggcaga atatccatcc actggttctg
     2401 gcggacctcc ataagaaatc caaagtgtcc cagattgatg cccaatatcg gcagacccaa
     2461 agggccgcat agatgcccag cgcgc
//
LOCUS       DBNHOH010000513         1830 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1248677, whole genome shotgun sequence.
ACCESSION   DBNHOH010000513 DBNHOH010000000
VERSION     DBNHOH010000513.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1248677"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="AC16V7_08965"
     CDS             complement(<1..342)
                     /locus_tag="AC16V7_08965"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR6257291.1"
                     /translation="MIKPYRFSTKIRVRFNETDAQGHVNFAWYLNYFDLALIEYLRTL
                     GYSYNDMLAEGLDMLYVDAHAAYQSPAHFDEVLRLHSRLGKLGNTSMRFEFQIFSEED
                     QRLVATGEITVV"
     gene            complement(379..1590)
                     /locus_tag="AC16V7_08970"
     CDS             complement(379..1590)
                     /locus_tag="AC16V7_08970"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR6257292.1"
                     /translation="MAFQKAYIPYGGYWSTPFAKWQGSFANLHPFHFAAQVTRRALEE
                     RNISPKEFTSLYLGTTVPAKQSFYGAPWMAGMVGADGITGPLFSQACATSARVIGSAA
                     FEVEAANGAETTLLCITADRTSNGPHLLYPNPLAPGGRGEAEDWVWDNFNLDPYARNS
                     MLQTAENVARELDISTAEQHQAVLIRYAQYQQALEDGAAFHKRYMITPVEINPSGRKV
                     VGTLEDDEGIFPTTAEGLARLKPVLPDGTITFGGQTYPADGNAGMIVTSRDKAAELSR
                     DPKIEIQLLAFAEGRAPKGYMPKANAPAVRRVLQLAGIALSDVKAIKTHNPFAVNDCF
                     LSRELGIPLEGMNNYGSSLIWGHPQGPTGMRLIIELIEELVLLGGGYGLFTGCAAGDT
                     AAAVVLEVRSR"
     gene            complement(1594..>1830)
                     /locus_tag="AC16V7_08975"
     CDS             complement(1594..>1830)
                     /locus_tag="AC16V7_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194853.1"
                     /note="catalyzes the thiolytic cleavage of
                     beta-ketoadipyl-CoA to succinate and acetyl-CoA; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HIR6257293.1"
                     /translation="QALAVMKELGLQHAITNVNGGAVALGHPLGCTGARIVCTLVHEM
                     RRRAAQAPSPFYGLATLCVGVGQGEATIFEWIGD"
BASE COUNT          378 a          512 c          529 g          411 t
ORIGIN      
        1 gaccactgtg atctcgccgg tagccaccag gcgttgatct tcttcgctaa aaatttgaaa
       61 ttcgaagcgc atgctagtgt tgccaagctt ccccagccgg gagtgcaaac gcagcacttc
      121 atcaaaatgg gctggcgatt gataagctgc atgggcatcc acatagagca tgtccaatcc
      181 ttctgctagc atgtcgttat agctataacc caacgtgcgc agatattcga ttaacgcgag
      241 gtcaaagtaa ttgagatacc aggcgaaatt aacgtgcccc tgagcatcgg tctcgttgaa
      301 acgcactcgt atcttggtgg agaatcgata tggtttgatc atcgcgttct gcgcttttca
      361 ctcctggtca tgtcatgatc acctagaacg gacctccaat accaccgcgg ctgcagtgtc
      421 accagccgcg cagccggtga acaatccgta cccgccgccc aagaggacta actcctcgat
      481 cagctctatg atcaggcgca taccggttgg cccctggggg tgcccccaga tcagcgagga
      541 accgtagttg ttcatccctt cgagcggtat gcccagctca cggctcagaa agcaatcgtt
      601 gacggcaaac gggttgtggg ttttgatcgc ttttacgtcg ctgagggcga tgcctgctaa
      661 ctggagcact cggcgaacgg ctggggcgtt ggctttgggc atgtaccctt tgggggcgcg
      721 cccctcggca aaggcaagca attggatttc aatcttaggg tcgcggctca attcagccgc
      781 cttgtcgcgg ctggtcacaa tcataccggc gttgccatct gccgggtacg tctgcccgcc
      841 aaaggtgatc gtcccatccg gcaggacggg cttgaggcgc gctaagcctt cggcagtggt
      901 ggggaaaatg ccttcatcat cttccagcgt gcccacgacc ttacgaccgg agggattgat
      961 ttcgaccggg gtgatcatgt agcgtttgtg gaaggcggcc ccgtcctcta aggcttgctg
     1021 gtattgcgca tagcggatta ataccgcctg gtgctgttca gcggtgctga tatcaagttc
     1081 gcgggctacg ttttcggcgg tttggagcat gctgttgcgc gcgtatgggt ccaggttgaa
     1141 gttatcccac acccaatctt ccgcctcgcc tcgcccaccc ggcgcaagag ggttggggta
     1201 gagcaggtga ggcccattgg atgtgcggtc cgcagtaatg caaagcaagg ttgtctcagc
     1261 accgtttgca gcttcgactt caaatgccgc gctgccgatc actcgagctg atgtggcgca
     1321 ggcttgcgag aagagcggcc cagttatccc atccgcgccg accatacctg ccatccaggg
     1381 ggctccgtaa aaggactgct tggcggggac ggttgtcccg aggtacaggc tggtgaactc
     1441 tttgggtgag atatttcgtt cctccagcgc ccggcgggta acttgagcag cgaagtgaaa
     1501 cgggtgcagg ttagcaaaac tgccctgcca tttggcaaac ggcgtgctcc agtagcctcc
     1561 gtatggaatg taagcttttt ggaacgccat cgattaatct cctatccact caaaaatggt
     1621 ggcctcgcct tgtcctacgc caacacacaa ggttgccaat ccgtaaaatg gggagggggc
     1681 ttgcgcggcg cggcggcgca tttcgtgaac taaggtgcag acaatgcgcg ctccggtaca
     1741 acccagcggg tgaccaagcg ccaccgcacc accattgacg ttggtaatgg catgctggag
     1801 gccaagttct ttcatcacag ccagcgcctg
//
LOCUS       DBNHOH010000514         2579 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1761615, whole genome shotgun sequence.
ACCESSION   DBNHOH010000514 DBNHOH010000000
VERSION     DBNHOH010000514.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1761615"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            346..1158
                     /locus_tag="AC16V7_08980"
     CDS             346..1158
                     /locus_tag="AC16V7_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257294.1"
                     /translation="MTTHVEITKSDHAIQVPVGIKPSIWPFVLVGILVLSLITAVIFV
                     ATPSTREVSANLRSLDAMSARYQGLADLHAAKNVANQRSLEATSARYQALASFFAAEN
                     IASARYQGLADFLAAKNVASQQSLEASTARYQGLAGFYTAANTADHPAVDVVSARYQG
                     LADFLTTEKVSSVKALEATSTRYQGLADIHAAKIVSDRLRALEATSARYQGMADDFAT
                     KKETSQRVLEATTARYQGLADFYAAKKGSNQRALNALSARLQGLADFYAAGK"
     gene            complement(1436..2416)
                     /locus_tag="AC16V7_08985"
     CDS             complement(1436..2416)
                     /locus_tag="AC16V7_08985"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6257295.1"
                     /translation="MQYLNLGRTGLKVSRLCLGMMTYGSTNWRAWVLDEEQGRPIIQK
                     AVESGINFFDTANAYSKGVSEEITGRALRDFARREEVVIATKVFFPYNEKPNQGGLSR
                     LHILRAVEDSLRHLGTDYIDLYQIHRYDPETPLDETLEALHDLVKSGKVRYIGASSMY
                     AWQFTKSLYLADLHNWTRFISMQNHYNLVYREEEREMIPVCLAEGIGIIPWSPLARGF
                     LAGNRSREDWGETIRAKTDGFGQTLYYRDADFQIVERVEEVAQQRGVSPAQIALAWIL
                     HKPGITSPIIGASKMYQLEEALAALEISLTPEEMTILEELYQPHPVLGHS"
BASE COUNT          645 a          718 c          606 g          610 t
ORIGIN      
        1 caactcaatc gcccgctcgt cgacctctat tctgaactgc ccaagcaggt atattttcaa
       61 cctcatgccc caccgtcaat tactaaggct ctccccaaaa tagaatacct aactgtttgt
      121 ttctgcctta ctcaggcact atatataaaa atttcgcggt tgaggaggtt ttcccctaaa
      181 ctgcgcaaga ccagttagat ttattatagc aaaaataatt ttctaatcca gaaaatacgc
      241 ttaaaaacgg cttcacatac ggatgcaaac gccctatttg ctatattgga accacaaaag
      301 acataacaaa cacaaaatat atcgctcaaa taaggagata ataaaatgac tacccatgtt
      361 gaaatcacga aatccgatca cgccatccaa gtcccagtcg ggatcaaacc atccatttgg
      421 ccgtttgtgc tggtaggtat tctggtcctt tctttgatca ccgcggttat ctttgttgca
      481 actcccagca cgagagaagt gtccgctaat cttcgttctc tggatgccat gtcagcccgc
      541 tatcaaggct tggctgattt acacgctgcc aagaatgtag caaaccagcg atctttggag
      601 gcaacttctg cccgctatca ggctctagct agtttctttg ccgccgagaa catcgcatct
      661 gctcgctatc agggtctcgc tgatttccta gctgccaaga atgtggcaag ccagcaatct
      721 ttggaggcat caactgcccg ctatcagggt ttagctggtt tctatacagc tgcgaataca
      781 gcagatcatc cagccgtgga cgtcgtatct gcccgctatc agggtctggc tgactttctc
      841 actactgaga aggtgtcaag tgtaaaagca ctggaggcca cttctacccg ctaccagggc
      901 ctggctgaca tacacgccgc caaaatcgtg tcagatagac ttcgagcact ggaagccact
      961 tctgcccgct atcaaggtat ggcggatgac ttcgccacca agaaagagac aagccagcga
     1021 gttctggagg ccacaactgc ccgctaccaa ggcctggccg atttctacgc cgccaagaaa
     1081 gggtcaaatc agcgggcgct caatgccctg tctgcccgcc tccagggtct ggctgacttc
     1141 tacgccgccg ggaaatgagg caggcttaag ggatcacctc tgccgtcagc gagatatggc
     1201 tgagatacac agatcgggag tgtgaaaatt gccaaatgta gcccaaagcg atcatcgcat
     1261 ctaacaaacc gctcgacatg tctgttgagc ggtttgttgt tcaatgaatt tacgccttcc
     1321 taatccagac tgccttctgg ttgagagaca agccaattaa atattaggac aagatcatct
     1381 gcgatgcggt gcaagcgctc tttgaccaga gattcgcagg tgccttttat acgaactaac
     1441 tatgacccaa aaccggatgt ggttggtata gctcctccaa aattgtcatc tcttccgggg
     1501 taaggctgat ctcaagcgct gccagcgctt cttcaagctg gtacattttg ctagcaccaa
     1561 taatcgggga tgtaatcccc ggcttatgca gaatccaggc tagcgcaatt tgagccggcg
     1621 atacgccacg ctgttgggca acctcctcca cacgctcaac aatttggaag tcagcatccc
     1681 gataatacaa ggtttggcca aagccatccg tcttggcgcg gatcgtctca ccccaatcct
     1741 cccgggaacg gttgcctgcc aggaagccgc gcgccagcgg gctccacggg atgatgccaa
     1801 taccctccgc caggcacacc gggatcatct cgcgctcttc ctcgcggtag accaggttat
     1861 agtggttttg catcgaaata aagcgcgtcc agttgtgcag gtcagccagg taaagcgact
     1921 tggtgaattg ccaggcgtac atgctggagg cgccaatgta gcgcaccttg ccagatttga
     1981 ccaggtcgtg cagcgcttcc agcgtctcgt ccagcggcgt ctccgggtcg tagcggtgga
     2041 tctgatacag gtcgatgtag tccgtaccca ggtggcgcag cgaatcttcg actgcgcgca
     2101 ggatatgcag gcgcgataaa ccaccctggt taggcttctc gttgtagggg aagaagacct
     2161 tggtagcgat gaccacctcc tcgcgccggg cgaaatcccg caaagcgcga ccggtgatct
     2221 cttcacttac tccctttgag tatgcgttgg cggtatcaaa aaagttgatc ccgctctcca
     2281 cagccttttg gatgatggga cgaccctgtt cttcatccag cacccatgcg cgccagttag
     2341 tagagccata cgtcatcata ccaaggcaca ggcgagatac cttcaggccg gttcgaccca
     2401 ggttgagata ctgcacgaaa acctcctctc tcaaaccaga aaacaatgtt taatgcgcac
     2461 ctcttccaaa cgcgccacta ttccaagttt accagcaccg cctggaacag tacatatttt
     2521 tattggctac taggaacggg atttgttcca gagctgaaca aaatatatca ttcgactac
//
LOCUS       DBNHOH010000515         3217 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1248713, whole genome shotgun sequence.
ACCESSION   DBNHOH010000515 DBNHOH010000000
VERSION     DBNHOH010000515.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1248713"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="AC16V7_08990"
     CDS             complement(<1..431)
                     /locus_tag="AC16V7_08990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR6257296.1"
                     /translation="MNRTTLRTLIRLAFKLLSRVTVYGLEWLPPSGGYILAVNHLSRV
                     DAPLVFSLIERNEVTALVAEKYKKRPFFRWIIRLVDGIWINRGEADLHALRQARDHLR
                     NGGVLGIAPEGTRSHTGALMRGKTGVAYLADKAGVPVIPTA"
     gene            746..1624
                     /locus_tag="AC16V7_08995"
     CDS             746..1624
                     /locus_tag="AC16V7_08995"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR6257297.1"
                     /translation="MFLILVAAVVLAGCSRETPAPSASPPPTTSIMASSTPTQSQPSP
                     TPAPLAATVNGEAILLSDYQAELSRYQADVGTELATEDQQRVLNELIDQLLLAQAAAE
                     AGFVVDETILQERYAGLVSQLGSDQALRDWMEANAYTEESFRRTLARSVAAAWMRDQI
                     AAEVPTALEQVHIRQILLYNSEEADRVIAQLQSGEDFADLAALNDPAAGGDLGWVPRG
                     YLLDAKLDEAAFSLQPGEFSAVIQTSAGYHILQVLEREPLLPLEFDARLALQRRAVQD
                     WLVARRGQSQIQVFTP"
     gene            1649..2398
                     /locus_tag="AC16V7_09000"
     CDS             1649..2398
                     /locus_tag="AC16V7_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257298.1"
                     /translation="MDSFDYFDRERPIHRIMNFIWNVLTIVMLLGVLCVAGYFLLVFT
                     NPDTPFNPLRPQPLPSPISLPTSTSTPRSILPPTWTPVPTLEPTDTPTPLPTDTPTPT
                     ETPFSLFTPTETATLEGARGMPFDVSPGTPVSTSSLAFHPEVNCDWMGVAGQVFDLSG
                     APISGQQVRIGGFLGSQPVSMLTLTGLTNAYGTAGFYEFKLADKPSATFGSMWVQLLD
                     QAGLAMSDKIFFDTFDSCEKNLIFVNFKQVR"
     gene            complement(2405..>3217)
                     /locus_tag="AC16V7_09005"
     CDS             complement(2405..>3217)
                     /locus_tag="AC16V7_09005"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HIR6257299.1"
                     /translation="PISTPSKPGGSVGAVLRSAELDLFALELGWVRAVRVSPETCAAI
                     QATAREQDVLISVHAPYYINLNANDEEWPKSRQRLMDAAHYGNLAGASDIVFHPGSYF
                     ERPPDEVLPTAISRLQGCIEELRQAGNPVTLRPETMGKSAMLGSLEDTLKMSQAIPGV
                     QPCLDFAHLHSRTGDGSMNTYDEWSGALDAYGKALGESALQNLHVHLSGIEYGEKGER
                     EHLPLQESDLDLGAILRALHAFGCQGRLLCESPVMEDDAAYMRQVWAEVSGE"
BASE COUNT          662 a          928 c          917 g          710 t
ORIGIN      
        1 attgcagttg ggatcaccgg cacgccggct ttatcagcca ggtaggctac cccggtcttg
       61 ccgcgcatca gggcgccggt gtggctgcgc gtcccctccg gggcgatgcc caacacaccg
      121 ccatttcgca gatggtcgcg cgcctggcgc aaggcatgca ggtcggcttc gccccggttg
      181 atccagatgc cgtctacgag gcgaatgatc cagcgaaaga acgggcgttt cttgtatttt
      241 tcagccacca gggcagtgac ttcattgcgc tctatcaaag agaaaaccag cggcgcgtcc
      301 acgcggctga gatggttaac tgccaggata tacccgccgc taggtggcag ccattccagc
      361 ccatagaccg tgactcggga gagcagctta aaagccaggc gaattaatgt gcgtagagtt
      421 gtgcgattca tcggtatgcc ggcaagatgt ccaagttgat actaatttta tcccgtattg
      481 gctgatgttg ccatgcttgc cagcagccta ccagcatagc aacccggccg ggcagccgtt
      541 ttttgatcag aattttcaac ctcaaatgac aactttaccc tcaagaatac taaagggttg
      601 ctgaaatcgg atcaaaccac ctcacccgtg cttataaata tagattcgct cctgctccag
      661 gtgcaaatgt gggggaaaca tgactaattg ttgattgtga tgcttcgcat aaataggtat
      721 aatcttccca atgaggaaac actgcgtgtt tctaatcttg gtagcggctg ttgtcttggc
      781 tggctgttcg cgcgagacgc cagcgcctag cgcctccccg cctccaacaa cgtctataat
      841 ggcctcgtca acgcccaccc aatcgcagcc ttcaccaact ccagctcctt tggctgcgac
      901 cgtcaatggg gaggcaatcc tgttgagcga ttaccaggcc gagctgagcc gctatcaggc
      961 ggatgttggc acggaactgg caactgaaga ccagcagcgc gtgttgaatg aattgatcga
     1021 ccagctcttg ctggcgcagg ctgcggcgga ggccggtttc gttgtagacg aaacgatctt
     1081 gcaagagcgc tacgcgggat tggtttctca gcttggtagc gaccaggcac tgagagactg
     1141 gatggaggcc aacgcctaca ccgaggagag cttccgccgg acgctggccc gctctgtggc
     1201 tgcagcctgg atgcgcgacc agatcgccgc cgaagtgccg accgcgctag agcaagttca
     1261 tattcgccag attttgctat ataattccga ggaggccgac cgcgtgatag cgcagttgca
     1321 atctggcgaa gatttcgccg atttagcggc acttaacgac ccggccgcag gcggcgatct
     1381 gggttgggtc cctcggggat acctgctgga tgcaaaatta gacgaagcgg cattcagctt
     1441 gcagcctggt gagttcagtg ctgtgatcca gaccagtgca ggctaccata tattgcaggt
     1501 tctggagcgc gagccactac ttcctctgga attcgatgcc cggttggcgc tgcaacgcag
     1561 ggctgtgcag gattggctgg tggcgcgtcg cggccaaagc cagattcagg tttttacacc
     1621 gtaaatccat cagtggaaag aggagagcat ggacagcttt gattacttcg atcgagagcg
     1681 ccctatccac aggatcatga acttcatctg gaacgttctg accatcgtca tgttgctggg
     1741 ggtgttgtgt gtggctggct acttcttgct ggtttttacc aacccagata cgccttttaa
     1801 ccccttaagg ccacagccgc taccttcgcc gatatccttg cctacctcca cgtccacacc
     1861 gcgcagcatt ttgcccccta cctggacgcc cgtgcctacc ctagagccca ccgatacccc
     1921 cacgccgctg cccacggaca cgcctactcc cactgagaca cccttcagct tgtttacccc
     1981 taccgagact gccacgctcg agggggcgcg agggatgcct ttcgacgtga gtccgggcac
     2041 gccggtcagc acctccagtc tcgcctttca cccggaggtc aattgcgatt ggatgggtgt
     2101 agccggacag gtgtttgatc tctccggcgc gccgatctct ggtcagcagg tgcgcattgg
     2161 cggctttttg ggtagccagc cggtgagcat gctcactctg acggggttaa ccaacgctta
     2221 cggcaccgcc ggattctacg aattcaaact ggccgataaa cccagcgcca cctttggcag
     2281 catgtgggta cagttactcg accaggctgg tctggcgatg tcggataaga tcttctttga
     2341 tactttcgat agctgcgaaa agaacctgat cttcgttaat ttcaaacagg tgcggtagaa
     2401 ggctttattc tccgcttact tctgcccata cttgtcgcat ataggcggca tcgtcttcca
     2461 taacagggct ctcgcatagc aagcgcccct ggcagccgaa tgcgtgcagg gcgcgcaaga
     2521 tggcgcccag gtccagatcg gattcttgca agggcaggtg ctccctttcg cctttttccc
     2581 catattcgat accggatagg tggacgtgca gattctggag ggcagattca cccaatgcct
     2641 tcccgtatgc atccaatgcg cctgaccact catcgtaggt gttcatggaa ccgtccccag
     2701 tacgggagtg caggtgggcg aaatccaggc agggctgtac gccgggtatg gcctggctca
     2761 tcttcaaagt gtcttcgagc gagccaagca tggccgattt ccccatcgtc tcagggcgca
     2821 aggtaaccgg gttgccagcc tggcgcaatt cttcgatgca gccctgcagg cgggatatgg
     2881 cggtcggcag cacctcgtca ggcggtcgct cgaaatatga tcctggatga aagacgatgt
     2941 cgctggcacc cgccaagttt ccgtagtggg cagcgtccat caaccgctgg cgagacttgg
     3001 gccactcttc atcattggca ttcagattga tgtagtaggg ggcgtgcacg ctgatgagca
     3061 cgtcctgctc ccgggcggtt gcctggatag ccgcgcaggt ctcaggggag acgcgcacgg
     3121 cgcgcaccca gcctagttcc agggcaaata ggtctagctc ggcggaacgc aacacagcac
     3181 ccacactgcc accgggcttg gagggggtgg agatggg
//
LOCUS       DBNHOH010000516        12743 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_672027, whole genome shotgun sequence.
ACCESSION   DBNHOH010000516 DBNHOH010000000
VERSION     DBNHOH010000516.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 12743)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12743)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12743
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_672027"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="AC16V7_09010"
     CDS             complement(<1..759)
                     /locus_tag="AC16V7_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012945370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HIR6257300.1"
                     /translation="MKSVNYQVVPYPKSRQPIVDYLTVARSKHTIHGILEFDVTAPRQ
                     VMREHKTRTGESLSFTAFIICCLAKAIDENKKLHAYRRGRNWLVIYDEVDISTPIERE
                     VKGHRIGTPYVVRSANAKTFREIHREIRIAQVEEAGAHTRMQRSGLLPNALIRLFWML
                     LAISPHLKKKYTGTVGITAVGMFGKGAGWGIPITDYTLQVTLGGIAEKPWVVDGRIEV
                     RDILSLTLSFDHDVVDRAPAARFAQRFKELVESGY"
     gene            complement(787..1650)
                     /locus_tag="AC16V7_09015"
     CDS             complement(787..1650)
                     /locus_tag="AC16V7_09015"
                     /inference="COORDINATES: protein motif:HMM:NF022441.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HIR6257301.1"
                     /translation="MYKRWIFILSMMVLFSFACQAVTGIGGVVRGSGEVVSETRQVSG
                     FDQISVCCGMQLIVTQGDVESLEIEADDNLLPEIVTTVSGGRLTIRFKDDQGRTQYWP
                     SQPIRCTVTAIAVRRLEVSGGGSLDAGLIETDDLQIDTSGGSQADVGSVVADTMRVEV
                     SGGGEFYAQDLQVNRLELDLSGGSTGSIDSLEGETLKLDNSGGGEVMVAGRVTEQDVS
                     ISGGGKHLAGDLKSETVVISMSGGGQAGLWVEESLDADLSGGARVEYYGRPRITEQVS
                     GGSELVSLGER"
     gene            complement(1766..3178)
                     /gene="radA"
                     /locus_tag="AC16V7_09020"
     CDS             complement(1766..3178)
                     /gene="radA"
                     /locus_tag="AC16V7_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIR6257302.1"
                     /translation="MPKSRTQYVCQQCGRVSPQYMGRCPQCQSWNSMVEEVVAPPSKS
                     KRSLVPGLVGRSQPTRLSEISGEADERLPLSIGEFARVLGGGVVPGSIVLIGGDPGIG
                     KSTLLLQVALAIAHHFPVLYVSGEESARQIKMRAARLLREAGDEGKPFPEDLYLVTET
                     NLDVILEHVTELKPRLLVVDSIQTTAMAELASSAGSVSQVRECASRLRELAKSSGIAV
                     FLIGHVTKEGAIAGPRVLEHIVDTVLYLEGDRFQSYRLLRSVKNRFGATSEVGVFEMG
                     EGGMAEVTNPSEAFLAERMVNAPGSAIAVTMEGTRPLLVEVQGLTSPTSFGNPRRTPN
                     GVDFNRLLLVAAVLTRRMGLRLGDQDVFVNVVGGLQVDEPAADLAVAAAIASSVRNLP
                     VRAETVLIGEVGLSGELRNVGQMPARLREAANLGFKSAIVPRRMRGAKPADPAGIQVV
                     EARSLKQALDMALLAEAKEK"
     gene            complement(3367..5859)
                     /locus_tag="AC16V7_09025"
     CDS             complement(3367..5859)
                     /locus_tag="AC16V7_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIR6257303.1"
                     /translation="MERITQRARRVLSLAHQEAERMRHNYIGTEHLLLGLIREEGGVA
                     GRVLRELGLEPERVQEIVERLTGTGQYRGGKLDLSPGTQQVLEYAVDEARRMGHHYIG
                     TEHLLLGLVRYGEGVAMDVLRKLGVTPEQIRRQTRRVLQESTASRRTSVGESQKTAKQ
                     ESQKPKTPMVDQLATDLTGLAEEDKLDPVIGRQMEIERVIQILARRTKNNPALIGEPG
                     VGKTAIVEGLAQRIVEGDVPAPLLSKRVLQLDVGSLVAGTMYRGQFEERLKRVIEELK
                     SSGAILFIDEVHMLVGAGAAGSSVDAANILKPALSRGELQVIGATTLDEYRKHIESDA
                     ALERRFQPIMVEEPSEEETIEILRGIRSAYEEHHRLTISDEALDAAVHLSARYVTERF
                     LPDKAIDLIDESSSRVRMYKSDAAQAAKELMRELRELRTNHAIALEDNRYDDAQDYLD
                     RQENLEMQLERMRTVWDRASSPVVSADDIAEVVSMWTGVPVMQIAKEESQRLLEMEKE
                     LGSHIVGQEEAIQAISKAVRRARAGLKDPRRPIGSFIFLGPTGVGKTELTKAMARFLF
                     GSEEALIQLDMSEFMERHSVSRLVGAPPGYVGYEDAGQLTEALRRRPYSIVVFDEVEK
                     AHPEAHNMLLQIMEEGHLSDARGHKVDFRNAIIVMTSNVGADMIKRQTSLGFAMKRDE
                     ETEERMAYEEMRKKLLDSLKRVFRPEFINRLDSVIVFRSLNKDDIKKIVELELNKVSQ
                     RLVEHDLHLRATPEALEMLADLGFDPEMGARPLRRVIQQKIEDRLSDALLSGEFKDGD
                     IISVEVVEGELKLDRSDVKEETPPEEAVATAS"
     gene            complement(6172..7995)
                     /locus_tag="AC16V7_09030"
     CDS             complement(6172..7995)
                     /locus_tag="AC16V7_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012784.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIR6257304.1"
                     /translation="MESNNALPGQGVEPESKESTEQKPEERPTTDLSTMAILLEEQGL
                     VDLPKTGEVRSGVIAAIGENEILVSVGTKSEGVISGRELDGIPAEERAEFAVGTEIPV
                     YVVTPEDTNGNVVLSYLRAREEKDWHDAEQMLEDEDGFESKIIGYNKGGLLVPLGRLR
                     GFVPASQISLVRRATLGGDSPDQRWGKMVGEPIKVRVIEVDRERHRLILSERAALQET
                     RETLKDRLLDELSEGDVRTGRVTSVADFGAFVNIDGADGLVHLSEISWERINHPNEVL
                     KVGQDVQVKVISVDHERRRIGLSIRQLQEDPWLKKIKGMREGQLVEATVTHLTKFGAF
                     ARLDEELEGLIHISELSEQRINHPKEVVKEGEVVTLRVIKIDPERRRIGLSRRKVDSP
                     AYSDLDWKMALAEEVQEVQETGAPQPSAEDEGQETIVSQVAAEEIVQEAEAIAETSVE
                     EETVEVMAEAKAEEETAEAGVEAQAEEEAAEPEVEASNEVVAEAQAEEETAEAEDEAQ
                     AEEEAAEPEVKASNEVVAEAQAEEETAEAEDEAQAEEEAAEHEVEASNEVVAEAQAEE
                     ETAEAEVEAQAEEKAAEPEVEASNEVVAEAQAEEETASKAE"
     gene            8311..9411
                     /locus_tag="AC16V7_09035"
     CDS             8311..9411
                     /locus_tag="AC16V7_09035"
                     /EC_number="6.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016851 - magnesium chelatase activity
                     [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase ATPase subunit I"
                     /protein_id="HIR6257305.1"
                     /translation="MPPIYPFTAIVAQERMRRALVLNAVDPRIGGVLIRGERGTAKST
                     AARALAALLPRVAVVTDCRFGCDPDRPTTWCTECRERVAVGEALPVELRTTPFINLPV
                     SATEDRVVGTLDIEKAIQKGERQFEPGVLASANRGLLYIDEVNLLDDHVVDVLLDSAA
                     MGMNIVEREGISFTHPARFILVGTMNPEEGDLRPQLLDRFALSVDIRGVRDARERVLI
                     MERNLAFESDPDRFREQWLLKEDELSHKIEQARTLVDQVTYSKRDLLSIAALSASLNV
                     EGHRADLVILKAARAHAAFEGRKSITDRDIALAAELALPHRIKRGPFHQAEVSIEDLE
                     ERIEQLQGASASPGEPEMVPDERQQESLEKKK"
     gene            9423..10355
                     /locus_tag="AC16V7_09040"
     CDS             9423..10355
                     /locus_tag="AC16V7_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR6257306.1"
                     /translation="MEDESKQTPSQPSPQQVFDTGSANWWDGGEKVKIGPVFTPRKIE
                     NPLDKMSRRQGGRRSRTRTDRKRGRYIQARPSPGKADDLAFDATLRAAAPFQKQRGEM
                     RKRVAFAIRSDDYQKKVRVKKAANLVLFVVDASWSMAVAERMSATKGAILSLLTDAYQ
                     RRDRVGLAVFQKDRSILVLPPTNSVQLAQRALADIPVGGKTPLSAGLFLAHQVIQRER
                     LAHPDVMPLIILLTDGAGNVSMSHLPPQEESHRIAEQIADDDIRCVVINMEHAAFDQG
                     LAQTLAEHLKAPCYTLSELKAEVLYQKVREEMAG"
     gene            10407..11951
                     /locus_tag="AC16V7_09045"
     CDS             10407..11951
                     /locus_tag="AC16V7_09045"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HIR6257307.1"
                     /translation="MKTMIRVTKSWIPGFLTAILLSAGALSACASRNPGLPTVANTPS
                     HTMEQPAPTTPVGGASLDLAPASPADATPSLTPQPVPKTRYTLTAVLDYAAHLLVVDE
                     RIDYTNASPDTLNELLLVVEPLHYPGTFNFKNIAWEDGTPLEDFRRDGSLLRLPLRAP
                     LAPGDRLALSLNYELTLPSPTPSSEVRPIPFGYTARQTNLVDWYPFIPPYKAGEGWLA
                     HPAGFFGEHTAYEIAEFDVSLMLVDARSDLVVAASAPAQMEGDWYRYSFADARNFAIS
                     VSPEYQVYTTTVGSTTVLSYAFAFHEQAGRAVLQTTAEALELYNRLFGPYPRSQISAV
                     EADFLDGMEYDGMYFLSNGFYNIYQGTPGEYLVAIAAHETAHQWFYARVANDQALEPW
                     LDEALCTYSERLYYENAHPDALAWWWEYRINYYEPQGFVDGSIYNPLGYRAYRDAVYL
                     NGALFLEDLRQQVGDQAFFAFLQDYVAQMSGRIATGDDFFDILKAHTDADLTPLFKTY
                     FEFSSQ"
     gene            complement(12073..>12743)
                     /gene="galE"
                     /locus_tag="AC16V7_09050"
     CDS             complement(12073..>12743)
                     /gene="galE"
                     /locus_tag="AC16V7_09050"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HIR6257308.1"
                     /translation="HAVKDIVFSSSCTVYGDPHKVPITEDFPLSATNPYGRTKLIIED
                     ILRDMYKADPSWNTALLRYFNPVGAHPSGRIGEDPNDIPNNLMPYIAQVAVGRLSQLS
                     VFGGDYPTPDGTGVRDYIHVVDLAIGHIKALEKLRQKPGLVAYNLGTGRGYSVLEVVA
                     SFERASGRVIPYQIVERRPGDIALTYADPAKANRELDWRAELDLDEMCADVWRWQSGN
                     PNGY"
BASE COUNT         2648 a         4007 c         3313 g         2775 t
ORIGIN      
        1 ataaccagac tcgaccagct ctttaaatcg ctgcgcgaag cgcgcggctg gcgctctatc
       61 aacgacatca tgatcaaagc tgagcgtgag gctaagtatg tcgcgcactt cgatacggcc
      121 atcaaccacc caaggctttt ccgcaatccc ccccaaggtg acctgcagcg tatagtcggt
      181 aatgggtatc ccccaacccg caccttttcc gaacataccc acggcggtga taccgaccgt
      241 gcccgtgtac ttcttcttca agtgcgggga tatcgcaagc agcatccaaa acagccggat
      301 gagggcattg ggcagcagtc cggatcgctg catgcgcgta tgggcacccg cctcttccac
      361 ttgggcgatg cggatctctc gatgaatctc ccggaaggtt ttcgcgtttg ctgaccgaac
      421 gacataggga gtcccaatcc ggtgaccttt gacttctcgc tcgatggggg tgctgatatc
      481 cacctcatcg tagatgacca gccagttccg cccccgccgg taggcgtgca gcttcttatt
      541 ctcatctatc gccttagcca ggcagcaaat gatgaaggca gtgaaggata gcgattcccc
      601 ggtgcgggtt ttgtgctccc gcatgacctg ccgcggcgca gtgacatcaa attcgaggat
      661 tccgtgaatg gtgtgtttgc tacgggcgac ggtaaggtaa tcgacaatgg gttgtcggga
      721 tttcggatag ggtacaacct gatagttaac tgacttcatt gatatgcctc tgtgaatgtt
      781 cgctgcctac cgctcgccca ggctgaccag ctcgctgccc ccagaaacct gctctgtgat
      841 gcgtggcctg ccataatact ctactctcgc acccccactg agatcggcat ccagggactc
      901 ttccacccac aatccagcct gtccgccacc agacatcgag atgacgaccg tctcgctttt
      961 tagatcccca gccaggtgct tgccaccgcc gctgatggat acatcttgct ctgtcacgcg
     1021 cccagcgacc atcacctcac ccccaccgct gttatccagc ttgagcgttt cgccttccag
     1081 cgaatcaatg gagcctgtgc tgccaccgct caaatctagc tccaggcgat tgacctgtag
     1141 atcctgggcg tagaactcgc ccccacccga aacctcaacc cgcattgtat ccgccaccac
     1201 tgaaccgaca tcagcttggc tgccgccgct ggtatctatt tgaaggtcat ccgtttcgat
     1261 cagcccggca tccaggctgc ccccacccga gacctccagg cggcgcacag cgatggcggt
     1321 caccgtgcag cgtatcggct ggctgggcca gtattgagtc ctgccctggt catctttaaa
     1381 gcggatggtc agccttcctc cagacacagt cgtaacgatc tccggcagca ggttgtcgtc
     1441 ggcctcaatt tccagggact ccacatcgcc ttgggtgacg atcaattgca tgccacagca
     1501 cacgctgatc tggtcgaaac cgctcacctg gcgggtttcg ctgaccacct ctcccgaacc
     1561 tcttacgaca ccccctatac ccgtgacagc ctggcacgca aatgaaaaca aaaccatcat
     1621 gctaagtata aaaatccatc gcttgtacat ctgtgactcc cttcccacat ctactttttg
     1681 tcttcaaacc catccccatc aactgagatc aaaatagagc atgttttaaa ccagccaatg
     1741 aatctcagag atatgatcaa acgctctact tttccttcgc ttctgccagc agcgccatat
     1801 ccagcgcctg cttcaaggag cgcgcttcca ccacctggat gcccgccggg tctgctggct
     1861 ttgcgccgcg catgcgccgg ggcacaatcg ctgacttaaa cccgaggttg gccgcctcgc
     1921 gcaggcgggc tggcatctgc cctacattac gcagctcacc cgataacccc acctcgccaa
     1981 tcagcaccgt ctccgctcgc accggcagat tgcgtaccga agaggcaata gctgccgcca
     2041 ctgccaggtc agccgccggc tcgtcaacct gcaagccgcc caccacattc acaaatacgt
     2101 cctgatctcc caggcgcaga cccatacgcc gcgtcagcac cgcggctacc agcagcaggc
     2161 ggttgaaatc caccccgttg ggtgtgcgcc gcgggttgcc aaaggacgtg gggctggtca
     2221 gtccctgcac ctccaccagc agggggcgcg tgccctccat ggtcaccgca atggccgagc
     2281 caggggcgtt gaccatgcgc tctgccaaga aggcctccga cgggttggtc acctcagcca
     2341 tgccgccctc gcccatctcg aatacgccca cctccgacgt agcgccaaag cgattcttca
     2401 cgctgcgcag caggcggtat gattggaagc ggtcaccctc caggtaaagc accgtatcga
     2461 caatgtgctc cagcacgcgc ggcccggcga tggcaccctc cttggtcacg tgaccgatta
     2521 ggaacacggc gatgcccgaa gacttggcca gctcgcgcag acgggaggcg cactcgcgca
     2581 cctgtgaaac cgaccccgcc gacgatgcca gctcagccat ggcagtggtc tggatcgagt
     2641 ctacgaccag caggcgcggc ttgagttcgg tgacatgctc caggatgaca tccaggttgg
     2701 tctcggtaac caggtacagg tcttcaggga aaggcttacc ctcatccccc gcctcgcgca
     2761 gcaatcgagc ggcgcgcatc ttaatctgcc gcgccgactc ctcgccagat acatacagca
     2821 cagggaagtg gtgcgcgatg gcaagcgcca cctgcagtag cagggtggac ttgccaatgc
     2881 ccgggtcgcc gccgatcaac acgatcgagc cgggcaccac cccaccgccc agcacgcgag
     2941 caaactcccc gatcgagagg ggcaggcgct cgtcggcctc cccgctgatt tcggacaggc
     3001 gtgtaggctg ggagcgccca accaaccccg gaacaaggct acgcttcgac ttggacggcg
     3061 gtgcgacaac ttcctcgacc atgctgttcc agctttggca ctgcgggcaa cgccccatat
     3121 actggggaga gacgcgcccg cactgctggc aaacgtattg agttcttgat tttggcatag
     3181 tggatattat agcaaagaca gatgacaagc gctcatatat ccgcagcagg cgcatatcta
     3241 ttagaggacc gcctttttgc tgaaaccgcg caatcccctg attgtgtacc tgatccccta
     3301 atagaaaaaa cgtcttgcag ggtttacgca cctgcaagac gttacgatca cgattagctt
     3361 attgagttaa gaggctgtcg ccactgcctc ttccggtggc gtctcctctt tcacgtcact
     3421 gcgatctagc ttgagctcgc cctctaccac ttccacgcta atgatatcgc cgtccttgaa
     3481 ctcaccggag agcaaggcat ccgatagccg atcctctatt ttctgctgga tcacgcggcg
     3541 cagaggtcgc gcgcccatct ccggatcgaa tcccaggtca gcgagcatct ccagggcttc
     3601 tggggtggcg cgcaggtgga ggtcgtgctc caccagtcgc tggctgacct tgttcagctc
     3661 cagctcaacg atcttcttga tatcgtcttt gttgagcgag cggaagacga tcaccgaatc
     3721 caggcggttg atgaactctg ggcggaagac gcgcttgagc gaatcgagca gtttcttgcg
     3781 catctcctca taggccatgc gctcctcggt ttcctcatcg cgtttcatgg caaaacccag
     3841 cgacgtctgg cgcttgatca tatcggcgcc cacgttagat gtcatcacga tgatagcatt
     3901 gcggaaatcc accttatgac cgcgagcatc ggaaaggtgt ccttcctcca tgatctgcag
     3961 cagcatattg tgcgcttctg gatgggcttt ctcaacctca tcgaagacca caatcgagta
     4021 ggggcggcgg cgcagggctt cggtgagttg tccggcatct tcgtaaccca cataacctgg
     4081 gggggctccc accaggcggc tcactgagtg gcgctccatg aactcggaca tgtcgagctg
     4141 gatcagcgct tcttcgctgc caaacaggaa gcgggccata gctttggtca actcggtctt
     4201 gcccacaccc gtggggccga ggaagataaa ggaaccaatc gggcggcgcg gatccttcaa
     4261 accagcgcgg gcacgacgta ccgccttgga gatggcctgg atggcctcct cctggcctac
     4321 aatatggctg cccagctcct tttccatctc cagcaggcgc tgcgactctt ccttggcaat
     4381 ctgcatcact ggcacgccgg tccacatgga aaccacctca gcgatgtcat ctgcagaaac
     4441 caccgggctg ctggcccggt cccacacggt gcgcatacgc tcgagctgca tctcgaggtt
     4501 ttcctggcga tccagatagt cctgggcgtc atcgtagcga ttatcttcca gtgcgatggc
     4561 gtggttggta cgcagctcac gtagctcgcg catcaattct ttcgcagcct gagcagcgtc
     4621 gcttttatac atgcgcactc gagatgatga ttcgtcaatc agatcgatgg ctttatctgg
     4681 caggaaacgc tcggtcacat aacgcgccga gaggtgcacc gccgcatcca gggcctcatc
     4741 gctgatggtc agacggtggt gttcttcata agcgcttcgg attccgcgca gaatttcgat
     4801 cgtttcctct tcactgggtt cttccaccat aataggttgg aagcggcgct ccagagccgc
     4861 gtcgctctcg atatgcttgc ggtactcatc caatgtggta gcaccaatca cctgtaattc
     4921 cccgcgcgaa agcgctggct tcaagatgtt ggcagcatcc accgacgatc ccgccgcgcc
     4981 tgcacccact aacatatgta cctcgtcgat gaacaggatg gctccagacg atttgagctc
     5041 ctcgatgacg cgcttcaggc gctcctcaaa ctggccgcga tacatggtgc ctgccaccag
     5101 ggagccaaca tcgagctgca agacacgctt gctcagcaag ggagcgggta catcgccctc
     5161 tacaatgcgc tgagccagcc cctccacaat ggccgtctta cccacgccag gctctccaat
     5221 caaagctgga ttattttttg tgcgccgggc caggatctgg atgacgcgtt cgatctccat
     5281 ctggcgcccg atcaccgggt ccaatttatc ttcttcagcc agaccggtca ggtcagtagc
     5341 aagctggtcg accatggggg tcttgggctt ctgcgactcc tgcttagctg ttttctgtga
     5401 ctctcccaca ctggtgcggc gggaggcggt gctctcctga agcactcgtc gcgtctggcg
     5461 tcggatttgc tccggcgtga cacccagctt gcgcagcacg tccatcgcga ctccctcgcc
     5521 atagcgaact agtcccaata ggagatgctc cgtgccgata tagtgatgtc ccattcgccg
     5581 ggcttcatct acggcatatt ccagcacctg ctgcgtccca ggggagaggt ccagcttacc
     5641 cccccggtac tgcccggttc cggtcaggcg ctctactatt tcctgcacgc gttctggctc
     5701 taaacccaat tctcgcaaca cgcgtccggc tacaccacct tcttcgcgaa tcaggcctag
     5761 aagcaagtgc tccgtaccga tgtaattatg gcgcattcgc tcagcttcct gatgggctag
     5821 gctgagcaca cgacgcgccc gctgtgtgat acgctccatg ccggccaatg gtttacctcc
     5881 tgaaacaaag aacgatcatt aagaccaaag acggtcctct caagattacg agacctttag
     5941 tccaattgga ttctaccaga gtttgcgcaa gggggatatt ttttaactga tcaagattgt
     6001 aagattaacg aaactcttag atagcatggc aggtatcagc tagcagcgcc cgcgaaatga
     6061 agttaatctt acacagacac atcgttgaag gcaaagattc ccaattgtgc aacccctgtt
     6121 atagagaaaa gcgcgccatt gctggcgcgc ctgaatccag tatgggatat cttattcggc
     6181 tttagatgcc gtttcttctt cggcctgggc ttcagctacg acctcattcg aggcttcaac
     6241 ttcaggttca gccgcctttt cttcagcctg ggcttcaact tcggcttccg cagtttcttc
     6301 ttcggcctgg gcttcagcta caacctcatt cgaggcttca acttcatgtt cagccgcctc
     6361 ttcttcggcc tgggcttcat cttcggcttc cgccgtttct tcttcggctt gggcttcagc
     6421 tacaacctca ttcgaggctt taacttcagg ttcagccgcc tcttcttcgg cctgggcttc
     6481 atcttcagct tccgcagttt cttcttcggc ctgggcttca gctacaacct cattcgaggc
     6541 ttcaacttca ggttcagccg cctcttcttc ggcctgggct tcaactccgg cttccgcagt
     6601 ttcttcttca gccttggctt cagccatgac ttcaacggtt tcttcctcaa ctgaggtttc
     6661 agctatggct tcagcttcct gaactatctc ctcggcggcg acctgcgaga caattgtctc
     6721 ctgtccctcg tcttcagcag acggctgggg ggcgccggtc tcttgcactt cctgcacttc
     6781 ttctgcgaga gccatcttcc aatccagatc tgaataggca ggcgaatcca ccttgcgacg
     6841 gctcaaaccg atgcgccgcc gctcgggatc gatcttgatc acccgcaggg tgaccacctc
     6901 gccctccttg actacctctt tgggatggtt gatgcgctgc tcgctcagct ctgagatatg
     6961 gatcaagcct tccaactcct cgtccaaacg ggcaaaagcg ccaaacttgg tcaggtgggt
     7021 gaccgtggct tctaccaact ggccttctcg catgcccttg atctttttca gccaggggtc
     7081 ttcctgcaac tggcggatgg acaggccaat gcgccggcgc tcgtgatcca cgctgatcac
     7141 cttcacctgc acatcctgcc ccaccttgag cacttcattg ggatgattaa tccgctccca
     7201 ggaaatctca gacagatgga ccagcccatc tgcgccgtca atattcacga aagcgccaaa
     7261 gtcggcaacg ctagtcaccc gaccggtgcg cacatcgcct tctgataatt catccaacag
     7321 gcgatccttt agggtctcgc gggtctcctg caaggcagcc cgctcagaga ggatcaggcg
     7381 atgacgctcg cggtctactt caatcacgcg caccttgatc ggctcaccta ccatcttgcc
     7441 ccagcgctgg tcaggggaat ctccgcccag ggtagctctg cgcaccaggc tgatctgcga
     7501 agcgggtaca aagccacgca atctgcccaa gggcactaat aagccacctt tgttataacc
     7561 gatgatcttg ctttcaaacc catcttcatc ttccaacatc tgctcggcat cgtgccagtc
     7621 tttctcttca cgggcgcgca gataggaaag caccacattg ccattggtgt cttccggcgt
     7681 caccacgtaa acagggatct ctgttcccac cgcgaattca gcgcgttcct cggcaggaat
     7741 tccatctagc tcgcggcctg agattacccc ttctgatttg gtgccaacgc tgaccaggat
     7801 ttcgttctcg ccaatagcgg cgatcacacc tgagcgcact tcacctgttt ttggaagatc
     7861 gaccaggcct tgctcttcca atagaattgc cattgttgaa agatcggttg ttggacgttc
     7921 ttctggcttt tgttcggttg attctttgct ttcgggctcc accccctgcc cgggaagggc
     7981 gttatttgat tccataagaa atgcttactc cggatactga ttaagatatg gcacacatta
     8041 taaagcgtta aaagaaagtt tgcaaccttt tgagacaggt gattagggca atcgcatttg
     8101 ggcgatttcc caccactgag gagagaaaat ggggtgtttt gcgggattgc gcatgaccct
     8161 cagggcatat gcaattgccc tggacaggtg atagagaact tgcccaaatg atcttaaaag
     8221 ggtagatcgg caacccatga tcccatatgc aaacatcctt ctcaaaagag ggtgaataaa
     8281 ttgagttata atgctaatcc gaggaagtac atgccgccta tttatccgtt tacagctatt
     8341 gttgcccagg agcgcatgcg gcgtgccctg gtgctgaacg cggtcgaccc gcgcatcggt
     8401 ggtgtgctga ttcgcggaga gcgcggcact gccaagtcaa ctgcggcgcg tgccttagca
     8461 gccttgctcc ccagggttgc agttgtcacc gattgccgtt ttggatgtga ccctgatcgc
     8521 ccgaccacct ggtgcactga gtgtcgtgag cgagtcgccg ttggcgaagc gctacctgta
     8581 gaactgcgca ccaccccatt tatcaacctg ccagtctccg ccacggaaga ccgagtcgta
     8641 ggcaccctgg atattgaaaa agccatccag aaaggcgagc gccaatttga gcctggcgtg
     8701 ctggcttctg ccaaccgcgg cttgctctac atcgacgaag taaacctgct ggacgaccat
     8761 gtggtagacg tgttgctcga ctcggctgct atgggtatga atattgtcga gcgcgaaggc
     8821 atctctttca cccacccggc tcgctttatc ttggttggga cgatgaaccc agaggaaggc
     8881 gacctgcgcc cgcaattgct ggatcgcttt gccctgtctg tggatatccg tggtgtacgc
     8941 gacgcgcgcg agcgcgtcct gatcatggag cgcaatcttg ccttcgaatc cgatccagat
     9001 cgcttccgcg agcagtggct gctaaaagaa gacgagctat cccacaagat tgagcaggcg
     9061 cgcacactag tagatcaggt gacttactcg aagcgcgact tgctttccat cgccgcattg
     9121 agcgcctcgc tcaacgtgga agggcaccgt gccgacctgg tgatcttgaa agctgcccga
     9181 gcgcacgctg cttttgaagg gcgaaaatcc attaccgacc gcgacatcgc cctggcagca
     9241 gagctggcgc tgccgcaccg aattaagcga ggtccattcc accaggctga ggtcagcata
     9301 gaggatctcg aggagcgcat tgaacaactg caaggcgcgt cagccagccc cggcgaaccg
     9361 gagatggtcc ccgatgaacg ccagcaggaa agcctcgaaa aaaaaaagta acgacggagg
     9421 tgatggagga cgaatctaag caaactccct cgcaaccgtc gccgcaacag gtcttcgata
     9481 caggtagtgc taactggtgg gatggtggcg agaaagttaa aatcggcccg gtcttcaccc
     9541 cccgcaaaat tgagaatcca ttagataaaa tgtcgcgccg ccagggtggg cgccgctcgc
     9601 gcacgcgcac cgaccgcaaa cgcgggcgct acatccaggc gcgtccctcc ccaggaaaag
     9661 ccgacgactt ggccttcgat gccaccttgc gcgccgctgc accattccag aaacagcggg
     9721 gtgagatgcg caaacgagtg gcgtttgcca tccgctccga tgactaccaa aagaaagtgc
     9781 gcgtcaagaa agctgccaac ctggtgctct tcgtggtaga tgcttcctgg tccatggcag
     9841 tagccgagcg catgtctgcc accaaggggg ccatcctctc cctgttaacc gacgcctatc
     9901 agcggcgtga tcgcgtggga ttagcggtct tccagaaaga tcgttccatc ctggtactgc
     9961 cccccaccaa ctccgtccag cttgcccagc gcgccctggc cgatatcccc gtgggcggta
    10021 agaccccgct ctccgcgggc ctgttcctgg ctcatcaggt catccagaga gaacggctgg
    10081 cccaccccga tgtaatgccc ctgatcatcc tgctcaccga tggggctggc aacgtgtcca
    10141 tgagccatct cccaccccag gaagaatctc accgtattgc cgaacagatc gctgacgacg
    10201 acattcgctg cgtggtgatc aacatggaac acgccgcatt cgaccaggga ttagcccaaa
    10261 cgcttgccga acatctcaaa gcaccttgct acaccctaag tgagctgaaa gctgaggtgc
    10321 tgtaccagaa ggtgagagag gaaatggcgg gttaggtccc cggagatttg caaatacatc
    10381 atcaggaaat tcggaatgca tgaacgatga aaaccatgat cagggtaaca aaatcctgga
    10441 ttcctggttt cctgacagca atccttctct cagcaggagc tctatcggct tgcgccagtc
    10501 gcaatcctgg actgcccacg gttgctaaca ccccgtcgca cacgatggag caacccgcac
    10561 caaccacgcc ggtaggagga gcatccctcg atctcgcccc tgcctccccg gctgatgcca
    10621 caccctcgct gacaccccag cctgtcccta aaacccgcta caccctgact gctgtattag
    10681 actacgctgc gcacctactt gttgttgatg agcgcatcga ttataccaac gcctcgcctg
    10741 acacgctaaa cgagctgctt ctggtggtgg agccactcca ctacccgggc accttcaatt
    10801 tcaagaacat cgcctgggaa gatggcacgc cccttgaaga ttttagacgc gacggcagcc
    10861 tattacgcct gccgctgcgc gcacccctgg caccgggcga ccgcctggcg ctttcgctca
    10921 actacgagct aaccctcccc tcccccacgc cctcctccga ggtgcggcct atccccttcg
    10981 gttacaccgc ccgccagaca aacctggtgg actggtatcc attcatcccc ccatacaagg
    11041 cgggagaggg ctggctggca catccggcgg gattctttgg cgagcacacg gcttatgaga
    11101 tcgccgagtt cgatgtcagc ctcatgctgg tcgatgcgcg cagcgacctg gtagtggcag
    11161 cctcggcccc tgcccagatg gaaggggatt ggtaccgcta cagttttgca gatgcacgca
    11221 attttgccat atctgttagc ccggagtacc aggtgtacac caccacagtc ggctcgacca
    11281 cggtgctcag ctacgccttc gcctttcacg agcaggctgg ccgggcagtc ctgcagacca
    11341 ccgccgaagc gctggaactg tacaaccggc tctttgggcc ctacccgcgc tcccagatca
    11401 gcgccgtgga ggccgatttc ctggacggca tggaatatga tggcatgtat ttcctcagca
    11461 atggcttcta caacatctac cagggaaccc cgggcgaata cctggtggcc attgccgccc
    11521 acgagaccgc ccaccaatgg ttctacgcta gggtggccaa cgaccaggcc ttggaaccct
    11581 ggctggacga ggccctgtgc acctacagcg aacgcctgta ttatgaaaat gcacaccccg
    11641 acgcgctggc ttggtggtgg gaataccgca tcaattacta cgagccacag ggattcgtgg
    11701 acggtagcat ttacaacccc ctcggttatc gcgcctaccg cgatgcggtt tatctaaacg
    11761 gagcgctctt cctggaagat ctgcgccagc aggtgggcga ccaggctttc ttcgctttcc
    11821 ttcaggatta tgtagcgcag atgtcaggca gaattgccac cggtgacgat ttcttcgata
    11881 tcctaaaggc gcacaccgac gccgatctaa ctcccctgtt caaaacctat ttcgagttta
    11941 gctcgcagta gttgaagaca gtctaccact gcgttgtcac cccaacacgt gtggagcaca
    12001 gctctatacc cctcagaggg cggtttctcg atgatctgac cgctattagg tcagtcacac
    12061 cgcccacaca acctaatatc cattcggatt ccccgactgc cacctccaaa cgtctgcgca
    12121 catctcgtcc agatcaagct cggcgcgcca atccagctcc cggttggctt tggctgggtc
    12181 agcgtaggtc aaggctatat cgcctggacg gcgttccacg atctgatagg ggatcacccg
    12241 tccgctggcg cgctcgaagg acgccaccac ttccagcacg ctgtagccgc gccccgtgcc
    12301 caggttatag gccaccaggc ccggcttctg gcgcagcttc tccaaagcct tgatatgccc
    12361 aatagccagg tccaccacgt ggatgtaatc gcgcacaccg gtgccatctg gggtgggata
    12421 atccccgccg aagaccgata gctggctcaa ccgacccacc gctacctggg caatataggg
    12481 catcaggttg ttggggatgt cgttggggtc ttcgccgatg cgcccggaag gatgcgcccc
    12541 aactgggttg aaatagcgca gaagtgccgt attccagctc ggatcggctt tgtacatgtc
    12601 gcgcaggata tcctcgatga tcaacttggt gcgcccgtag gggttggtcg ccgagagagg
    12661 gaaatcttcc gtgatgggca ccttatgcgg atcgccgtac actgtgcagg acgagctaaa
    12721 gacaatatcc ttgaccgcgt gtt
//
LOCUS       DBNHOH010000517         1666 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1442140, whole genome shotgun sequence.
ACCESSION   DBNHOH010000517 DBNHOH010000000
VERSION     DBNHOH010000517.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1442140"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="AC16V7_09055"
     CDS             <1..809
                     /locus_tag="AC16V7_09055"
                     /EC_number="2.7.7.-"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR6257309.1"
                     /translation="GNDEVMVLAHAFNYMVSGLQEGFIYRDLLGRTVSPEVREALRES
                     FALGKLRLEGQNTVATVMMSDIRGFTSLSEKEDPTIILKWLNEYYQVVVPIITSFGGV
                     VDKFEGDAMLSFFGILPKMLPAEDSAYLACKAGLELLRIIEELNTKRASRGDPPLVTG
                     VGINTGNLIAGGLGTADRLNYTIIGDAVNTTQRIEGVTRGFYESGIVISETTLTALKD
                     HRSEFYFEPLGEHALKGKSELIWLYRLWPENVGKHSQVRFESEVNDEVQS"
     gene            complement(1428..>1666)
                     /locus_tag="AC16V7_09060"
     CDS             complement(1428..>1666)
                     /locus_tag="AC16V7_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257310.1"
                     /translation="GTGVFVGGGFVGIGVLVGGGFVGIGVFVGGGCVGIGVDVGGGGV
                     RVGVGAIDVGGFVVGSDVLVGDTAVNEVDSVAVC"
BASE COUNT          447 a          425 c          383 g          411 t
ORIGIN      
        1 aaggcaacga tgaagtgatg gtattggcgc acgccttcaa ttatatggtt tctggcttgc
       61 aggaaggttt tatttaccga gatttgctag gtcgcactgt ttctcctgag gtccgggaag
      121 ctttgcgaga gtcgttcgca ttggggaaac tacgtcttga agggcagaac acggttgcta
      181 cagtcatgat gagcgatatc cgtggattca catccttgtc cgagaaagag gatcccacaa
      241 ttatcctgaa atggttgaat gaatactacc aagttgtggt gcccattatt acctcatttg
      301 gcggtgtggt ggacaaattt gagggcgatg caatgttatc gttttttgga atattgccca
      361 aaatgttgcc agctgaggat agcgcatacc tagcctgcaa agctgggctg gagttgcttc
      421 ggattattga agagctaaat acaaaacgtg catctcgagg agacccacct ctagttaccg
      481 gtgttgggat taatacaggc aatttgatcg caggtggatt gggaactgcg gatcggttga
      541 attatacaat tattggtgat gcagtgaaca caacccagcg aatcgaaggc gtcacgcgcg
      601 gtttttatga aagcggcatt gtgattagcg aaacgacgct gacggcttta aaagatcatc
      661 gcagtgaatt ttattttgag cctttgggtg agcacgcctt aaagggcaag agtgaactca
      721 tctggttata tcgcttgtgg ccagaaaatg ttggcaaaca ttctcaggtt cgctttgaat
      781 cagaagtcaa tgatgaagtt cagtcgtaag agtttatggg agcacctgcg ctgggtctct
      841 cttttcttga tactgttctt tgctttccta atgatcgttt tgattccact gcttgtgagg
      901 gttagccagc catattctgt gccatatgcc cttcactcag ttttgagcgc caattatggc
      961 gctgatccac acaacctgaa gcatgctcct cttcggctga gtatcatcgc ggatgtaata
     1021 cgagaccagc aggcaatcgc ctcgtctagg ttatcgctta atgaagttgg gagcgcgagc
     1081 gactcggaag tcccagcttc aagtaccgac tcttatccag taattaactc gacaccgggg
     1141 tccggttcag acaataatca gaatacacca gttgctgcgg atctagcgtc gacacagagg
     1201 agcgatactt attccactct ccaggcaagt gggacggtcg actcaacagg tataggttct
     1261 acacccacct taaagttcac gcaggaacct gctcaaacat caataattaa tattagaaca
     1321 ccaactccta cgcccaacag gacgggtcaa ataacaccgg tgtgtactgg gcaatctcca
     1381 acagcaaaac cggctgtaac aaatgcgccg acacaaacga cttctgctca gcaaactgcg
     1441 acactatcga cttcattgac tgcggtatcc cccactaaaa catcgctgcc aaccacaaaa
     1501 ccgccgacat ctatcgctcc aaccccaaca cgcactcccc cgcctcccac atcgacgcca
     1561 atcccgacac aaccaccccc aacaaatacc ccgatcccca cgaagccacc gcctaccaat
     1621 acaccaatac ctacgaaacc accgcctaca aatacgccgg ttccta
//
LOCUS       DBNHOH010000518         2898 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_929133, whole genome shotgun sequence.
ACCESSION   DBNHOH010000518 DBNHOH010000000
VERSION     DBNHOH010000518.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_929133"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(270..1223)
                     /locus_tag="AC16V7_09065"
     CDS             complement(270..1223)
                     /locus_tag="AC16V7_09065"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family RNA-guided transposase"
                     /protein_id="HIR6257311.1"
                     /translation="MTTPSEAYVGIDVGKTWLDIARWGSETVWRVSNDDDGVAKIMAW
                     VAEMEPQLIAVEATGGYEQLLVQSMLLNNLPVAVINPTRVRALSKATGKLAKTDVIDA
                     RLIAEYAFKIQPETLGPKEANEIRLNALVTRREQLVEMRVAEQNRLGTAQNSMQADIR
                     EHIEWLSSRIEALEAQINALVDSLPAWREQVQRLDSIPGVGRITAVTVLSEMPELGQL
                     DRQKIAALAGLAPFNRDSGHQRGKRRIFGGRKGIRRVLYMACLSAKKYNPVIRSLFER
                     LMKKGKVFKVAITACMRKLLTIMNAMTRDQVNWRTPKPISV"
     gene            1627..2616
                     /locus_tag="AC16V7_09070"
     CDS             1627..2616
                     /locus_tag="AC16V7_09070"
                     /inference="COORDINATES: protein motif:HMM:NF015971.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HIR6257312.1"
                     /translation="MDTKLSTGHKFKRLFAKILRFFAILAFASVSTGFSVGAAPSDQT
                     ADWEASGLAKPSVLHETSGYKIWYDGGFFDGNIQIGLARSRDGLNWKKAPQNPVLDGD
                     PDAWDAASGEHAPFVMKDGNLYKMWYEGSDGNVRQLGYATSHNGIKWTKYPGNPVLQA
                     GPEAYDQLVAGHGTLLFEDGIYRLWYHAIGDLGAIIAYATSPDGIHWTKHGPVLVGAP
                     EGWDTSLWGPSVLKVEGTYWMWYSAGGPLYPISIGAATSPDGVLWTRMGDHPVIADPE
                     FINNFMDPHVVFDAGLFKMWTGNFSDFVIYYAESENGTDWSKPVPVLFPGTSK"
BASE COUNT          633 a          780 c          793 g          692 t
ORIGIN      
        1 tgacgacctg aaacttggat gatatcgtcg tcgcttccat ttcttacgtc tccttatcga
       61 acagaaactg taagacaatt atagcacaac tagacgatcg ttcggtattt ctttgatggc
      121 tttctcaaag tcagtgaatt ctacgcggac gtcactgcga ggagcgtttt ctgcgacgaa
      181 gcagtctcca actcggttgc gaacgagatt gcttcgcctg ccagattgcg gcaggctcgc
      241 agtaactgtg tttttagtaa agatggaatt catactgaaa taggcttagg tgtcctccaa
      301 ttgacctgat cgcgggtcat ggcattcatg atggtgagca acttacgcat acatgcggtg
      361 atagcaacct tgaatacttt gcctttcttc atcaaacgct caaacaagga tcgaatgaca
      421 ggattgtact tcttagcgga caggcaagcc atatacagta cgcgacggat acctttgcga
      481 cccccgaaga tgcgccgctt accgcgctgg tggccactat cccggttgaa gggggccagg
      541 ccagccaagg cagcaatctt ctggcggtcc agctggccca actccggcat ttcggacaac
      601 accgttacgg cagtgatcct gcccacgccc ggaatgctgt ccaggcgctg cacttgttct
      661 cgccatgcag gcagactgtc aaccagcgcg ttgatctggg cttcgagcgc ctcgatgcgt
      721 gaactcagcc actcgatatg ttcgcggata tcggcttgca tgctgttttg ggcggttccc
      781 aggcgatttt gctcggcaac ccgcatctct accagctgtt ctcgccgggt gaccaacgca
      841 ttgagacgga tttcattggc ttctttcggc ccaagggtct caggctggat cttgaaggca
      901 tattcagcga tcagccgcgc atcgatcacg tccgttttgg ccagcttgcc ggtggctttg
      961 gataatgccc tcacccgcgt gggattgatc accgcgacgg gcaggttgtt caagagcatg
     1021 ctctggacga gcaattgctc atacccgcca gtggcttcca cggcgatcag ctggggttcc
     1081 atctctgcaa cccaagccat gattttggcg acgccatcat catcattgga tacccgccag
     1141 actgtttcgc tgccccaccg ggcgatatcc aaccaggtct tgccgacatc gatcccaaca
     1201 tatgcttcac tcggtgttgt cattggtacg attcctccgt ataatcgaga tgagcccaca
     1261 ctagcaagga ttcggcctct ctcggccact caactgttcg ggctcgcttg gatgttggac
     1321 atggcggtcg tactgcgaga cggtctacag gacctaatga ctagcggcct gccatgtcca
     1381 cttttattat catactaatg acatccgttc gcgactttga gaaagccatg attcagggca
     1441 attgtgcgct ttttcattaa tttgatttgg cgctaataat cgattataaa cataacgaaa
     1501 cacatcttca tcatcagtaa taaatcaaag gaggccaata aagacgaagt tcgttacagc
     1561 cggctgacag ggtttgcctg gttgagccgt gttcgtatct actgaatgac cataggagag
     1621 ttcaaaatgg atacaaaact ttcaacaggc cataagttca agaggctgtt tgcgaaaata
     1681 ctgcgattct ttgcaatcct ggcgtttgct tcggtcagca cagggttctc tgtgggtgca
     1741 gcgccgagcg accagaccgc tgactgggag gcgtccggct tggcgaagcc gagcgtgctc
     1801 catgagacga gcggctacaa gatatggtac gacggcggct ttttcgatgg aaatattcag
     1861 atcggcctgg ccagatcgag ggatggcttg aactggaaga aagccccgca gaaccctgtt
     1921 ttagatggcg acccagatgc ctgggatgct gcttccggag agcatgcgcc gttcgtcatg
     1981 aaagatggca acctgtacaa gatgtggtat gaaggctccg atggcaacgt gaggcagttg
     2041 ggctacgcaa ccagccacaa tggcatcaaa tggacgaaat atccaggaaa tccagtcctt
     2101 caagccggac cggaagcgta tgatcagctt gtcgccgggc atggcacgct gctttttgag
     2161 gacggcattt atcggctctg gtaccacgcc attggtgacc tgggcgctat tatcgcctat
     2221 gccacctcgc cggatgggat ccattggacc aagcacgggc ccgtcttggt gggcgcgcca
     2281 gaaggctggg ataccagctt gtggggaccg agcgtgctga aggtggaggg cacctactgg
     2341 atgtggtact ccgcaggcgg cccgctatat cccatctcca tcggtgccgc taccagcccc
     2401 gacggggtcc tatggacgcg catgggagat catcccgtca tcgccgatcc tgagttcatc
     2461 aataacttta tggatcccca cgtggttttc gacgcagggc tgttcaaaat gtggaccggt
     2521 aacttcagtg attttgtcat ctactatgca gaatctgaaa atggtaccga ctggagcaag
     2581 cctgtccctg tcctgttccc cggaaccagc aaataggata agcaagtagg aagggctctt
     2641 ccattcctgg aagatcattc gtcccggctg tgggaggggt aaggggtggg ggagcaccta
     2701 catcccgtat aatagcggca tgacctcgcc gattcaacct ccaggccgcg ccgagcgcct
     2761 gaaagctgat gcgctgctgc tgctggtcgc catcatctgg ggcacggcct ttgtcgccca
     2821 gcgcgtggcg gcgctgaaca tgggcttgta tgcctttaac ggcctgcgct tccttctagg
     2881 ggcgctggtg ctgctgcc
//
LOCUS       DBNHOH010000519         5483 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_736561, whole genome shotgun sequence.
ACCESSION   DBNHOH010000519 DBNHOH010000000
VERSION     DBNHOH010000519.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5483)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5483)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5483
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_736561"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      140..251
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            288..947
                     /locus_tag="AC16V7_09075"
     CDS             288..947
                     /locus_tag="AC16V7_09075"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HIR6257313.1"
                     /translation="MTTPTRAVIFANGVLNHPETALAALRPGDLIIAADGGARLCRQL
                     GLVPAILIGDFDSIDEADLAYFDANGTQIIRHPARKDYTDLELALHHVKSLGIQDVLV
                     VAALGARWDQTLANLLLPAAVDLDDLTIRLMDGPQEVVLLRGGDEIDIHGKPGDTVSI
                     IPVGGDVHGVVTDGLEYPLFNESLFFGATRGLSNVLLVERATIHLEQGLLLCVVIHQD
                     G"
     gene            1008..2189
                     /locus_tag="AC16V7_09080"
     CDS             1008..2189
                     /locus_tag="AC16V7_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR6257314.1"
                     /translation="MFKNISLIRSNCRYAPPEGRRGNLLLDFFAWPQKCWPLSILLLF
                     LILGLSACAPASLAPTSTGELPTVTPSGPRTLTVMTHDSFAVSDEVILAFESAYSVAV
                     KILPSGDTGTALNKAILSKGNPIADIFYGVDNTFLSRALDEDIFEPYDAPGLADVPEA
                     FLLDPGKRALPVDYGDVCPNYDKTYFSEKGLAPPRTLEDLAKAEYKGLLVVENPATSS
                     PGLAFLLATIGRFGADGYLDYWKSLVENDVLVVNDWESAYYTEFSAHGGTRPIVISYG
                     SSPPAEVIFAESPIEESPTAVIAAGRSCFRQIEFAGILKGSQNRDLAELWMDYMLSKT
                     FQEDMPLQMFVFPVSRQAVLPDEFAKYTSIPADTAQVNPDEIAANREQWLNSWTEAVL
                     R"
     gene            2203..3912
                     /locus_tag="AC16V7_09085"
     CDS             2203..3912
                     /locus_tag="AC16V7_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6257315.1"
                     /translation="MKLRTVYFLWLAPIAFLAIFFFYPLGSILAVSFGRALGEDMLEL
                     PRALLSPSIRRVVAFTFGQATLSTLLTLLMGLPGAYLIARYEFRGKSLFQALSGIPFV
                     LPTLVVAAAFNALLGPRGWINLALMDWFNLTQPPIQFLNTFTAIIVAHVFYNLTIVLR
                     IVGDFWSHLDPRLVQASQMLGASRLQTLLRITLPLLAPAIAAAALLVFIFDFTSFGVI
                     LILGGPRFATLEVEIYYQAISLFNLPMAATLALLQLVCTLAMTTIYTRLAAGLARPLG
                     LRPRRFTQRRLVTWRSRLVAALLVGLMSVFLITPLAALATRSFTRVDAGSQLVGSAGD
                     ITLDWTLDYYRELSINRRGSLFFAPPSTAIAVSLGYAAATVVLALLLGLPASWALARH
                     QSSALSRLLDPILMLPLGTSAVTLGLGFIVALDQPPLDLRASPVLVPLAHTMVAFPFV
                     VRSLTPSLRSIRPRLRQAASVLGASPGQVVRTIDLPMVGRALLVAATFAFTISIGEFG
                     ATALIARPEYPTIPIAIYRYLGMPGALNYGQALALSTILMGVTAAGMLAIERFRIAEV
                     GEF"
     gene            3915..4964
                     /locus_tag="AC16V7_09090"
     CDS             3915..4964
                     /locus_tag="AC16V7_09090"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018839603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6257316.1"
                     /translation="MNTKLGLGVQGIHKTFGEIRAVSGVSFDVDPGEVMALLGPSGCG
                     KSTVLAVIAGLEQADAGGISWDGKSLREVPPHERGFGLMFQDYALFPHMNVFGNIVYG
                     LRMRGWPEDRLRKRVGDMLELVGLPGFDKRDVNTLSGGEQQRVALARSLAPEPRLLML
                     DEPLGSLDRNLRERLVSDLRQILKRMHQTAIYVTHDQEEAFALADRVAVMNAGRLEQL
                     DAPQMIYARPASPFVARFLGMTNLLDGVIRPTLDGIVVETAVGAFPVVGQFQGRVTLL
                     LRPDAVRPDGVGSMHLQGRLLGCSFRGSSCQATIEVDGVPLKFDFLTSTPLPPAGELV
                     TLGFEPGDALQVFSQ"
     gene            5048..>5483
                     /locus_tag="AC16V7_09095"
     CDS             5048..>5483
                     /locus_tag="AC16V7_09095"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: protein motif:HMM:NF016085.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016891 - RNA endonuclease activity
                     producing 5'-phosphomonoesters, hydrolytic mechanism
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease FAU-1 family protein"
                     /protein_id="HIR6257317.1"
                     /translation="MSSITVIKLDINGVETWRYPGQLLHKQDTQIVLEAYFDRDDMIF
                     HGMPLHRGDRFLELYFTDRWYNIYEIHSRADDHLRGWYCNVCTPAQLDGDRLFFKDLA
                     LDLLVFPDGNQIVLDEDEFARLELSPDGRRLALAALDELRAGF"
BASE COUNT         1005 a         1497 c         1559 g         1422 t
ORIGIN      
        1 agtcgatgtg agggatgatc aattttcatc cacttggcct aagtggctcc ttgcatttgc
       61 tccaaggggt gtatgggaat ttattttccc gctttccttg acaacggcta acgctcgcaa
      121 tatactatct gtacaactaa atccttgcac ggggagcctg gtgatgccgg gctgagagga
      181 gggccgacag ccctcgaccc gcgaacctga tccggataat gccggcggag ggaacgcaaa
      241 tatcggaaaa atcacccctc ctgccgagcc aacaggaggg gttgcttttg accacaccca
      301 cgcgtgccgt catctttgct aacggggtgc tgaaccaccc tgaaacagcc ttggctgcct
      361 tgcgccccgg cgatctaata attgccgccg atgggggcgc gcgcctgtgc cgtcagttgg
      421 gcttagtgcc tgccatcttg attggcgact tcgactcgat cgacgaggct gacctggcgt
      481 attttgacgc caatgggaca cagatcatcc gccaccctgc gcgcaaggat tatacagatc
      541 tcgagcttgc tttgcatcat gtcaaatccc tgggcatcca agacgtcctg gtagtcgcgg
      601 cgttgggggc gcgttgggat cagacgctgg ctaatttgtt gctcccggcg gctgtcgacc
      661 tggatgatct gaccattcgc ttgatggacg ggccgcaaga ggttgtgcta ttacgcggcg
      721 gtgatgagat cgatatccat ggaaagcctg gcgacaccgt atctatcatc ccagtggggg
      781 gtgatgtgca tggggtagta accgacggtt tagaatatcc cttgttcaat gaatccttgt
      841 tctttggcgc cacgcgcggc ctgagcaacg tgctgctggt tgagcgtgct accatccatc
      901 tcgagcaggg gttgttgtta tgtgtagtga tccatcagga tggatgatga aattcgctgc
      961 tttttcgcac tatcgtcatt catcctgggg acgaaacgga ggtaagaatg tttaaaaata
     1021 tctcccttat tagatcaaat tgtcgttatg cgccccccga agggcggcgt ggtaatctcc
     1081 ttctcgattt ttttgcgtgg ccccagaagt gctggccgct ttctattctc ttgctcttct
     1141 tgatcttggg cctgtctgcc tgtgctcctg cgagcctggc gccaacttcg actggtgagc
     1201 ttccgacggt caccccaagc ggcccacgaa ccttaaccgt gatgacccat gattcctttg
     1261 ctgtctccga cgaggtgatc ctggcttttg agagcgccta cagcgtggcg gtaaaaatcc
     1321 tcccctccgg tgatactggc accgcgctga acaaagccat cctctcgaag ggcaacccta
     1381 tcgcggatat tttctacggt gtggataata cctttctcag ccgcgccctg gacgaagata
     1441 tctttgaacc ctatgatgcc cctgggctgg cagatgtccc cgaggctttc ctgctcgacc
     1501 cgggtaaacg cgctcttcca gtggattacg gtgatgtgtg tccaaactac gataaaacct
     1561 acttctctga gaagggattg gcgccaccca ggacgctcga agacctggct aaagcggagt
     1621 ataagggttt gctggttgtg gagaatccgg ctacttcatc gcctggactg gccttcctgc
     1681 tggctaccat tgggcgcttt ggggcagatg gctacctgga ttactggaaa tccctggtgg
     1741 agaatgatgt actggtggta aacgattggg aaagcgccta ctacactgag ttcagtgctc
     1801 atggcgggac gcgtcccatc gtcatttctt atggctccag ccccccagcc gaggtgatat
     1861 ttgcggagtc gcccatcgaa gagtcgccta ccgcggtcat cgctgcaggg aggagttgtt
     1921 tccgtcaaat cgaattcgcg ggcatcttga aagggtcaca gaatcgtgat ctggccgagt
     1981 tgtggatgga ttacatgctt tccaagacgt ttcaggagga tatgcccttg cagatgtttg
     2041 tcttccctgt cagccgtcag gcggtgctgc ccgatgagtt tgccaagtac acctccatcc
     2101 ccgctgacac ggcccaggta aacccagatg agatcgcagc aaatcgcgag cagtggttga
     2161 atagctggac ggaggcggtg ctcagataat ggcgttagtt atatgaagct tcgcactgta
     2221 tattttctct ggttggcacc catagccttc ctggcaatat tttttttcta ccctttgggc
     2281 agtattctgg cggtcagttt cgggcgtgcc cttggagagg atatgcttga acttccgcga
     2341 gcgctcttat caccttccat tcgccgtgtg gtggcattta cctttggaca ggcaaccctc
     2401 tccacgctgc tgaccttgct gatggggtta cccggagcgt atctgatcgc ccgctatgag
     2461 tttcgcggca aatcgctctt tcaggcgctc agtggtatcc cctttgtgct gcccacgctg
     2521 gtggtcgccg ctgcctttaa tgcattgctc gggccgcgcg gctggatcaa cctggcgctc
     2581 atggattggt ttaacctgac ccagccaccc atccagttcc tcaacacctt tacggctatc
     2641 atcgtcgccc atgtttttta caatttaacc attgtgctgc gcattgtggg tgatttttgg
     2701 tcgcacctcg atccgcgcct ggtgcaggcc tcccagatgc tcggagccag tcgactgcag
     2761 acgctgttac gcatcaccct gccgttgctg gctccggcta tcgcagctgc ggcgttgctg
     2821 gtattcattt ttgatttcac gtcttttgga gtgatcctga ttttaggagg cccgcgcttt
     2881 gccacgctgg aggtggagat ctactatcag gcgatcagct tgttcaacct gcctatggct
     2941 gctaccctgg cgttgctcca actggtttgc accctggcga tgaccactat ctacacccgc
     3001 ctggcagctg ggttagcacg cccattgggc ctgcgcccgc ggcgctttac ccagcgccgt
     3061 ctggtaacct ggcgtagccg cttggtagct gccctgctgg ttggcttgat gtctgtcttc
     3121 ctgatcactc ccctggctgc tcttgctact cgttcattca cgcgagtgga cgctgggagt
     3181 cagctagtcg gaagcgcggg cgacataaca ttggattgga ccttagatta ctaccgcgag
     3241 ctctctatca accggcgcgg gtcgctcttt tttgccccac cctcaacggc gatcgctgtg
     3301 tctttgggat atgccgcggc cacggttgtc ctggcgttgc tcctcgggtt gccagcctct
     3361 tgggcgctcg cccgccatca gtcttcagcg ctaagtcggc tgctcgatcc gatcttgatg
     3421 cttccgctgg gcacttcggc ggtcactttg ggattgggtt tcatcgtcgc cctggaccaa
     3481 cccccactcg acctgcgcgc ttcgccggtg ctggtgcctc tggcgcatac catggtggct
     3541 tttcccttcg ttgtgcgcag cctgacgcct tccttgcgca gtattcgccc gcgattgcgc
     3601 caggcagctt cagtattggg ggcttcccct ggccaggttg tgcgtaccat tgacctgccc
     3661 atggtggggc gggcattgct ggtggcggcg acgtttgcct tcacgatctc catcggtgag
     3721 tttggcgcga ctgcgctgat cgcccggcct gaatatccaa ccatcccaat tgccatttac
     3781 cgctatctgg gcatgccagg tgcgctcaat tatggtcagg cgctggcgct gagcacgatc
     3841 ttgatgggcg tcaccgcggc gggcatgctg gcgattgaaa gattccgcat cgcggaagtt
     3901 ggggagttct agtattgaat acaaagttag ggcttggcgt acaagggatt cacaaaacgt
     3961 ttggagaaat ccgtgccgtg tcaggcgtct cctttgatgt agaccctggc gaagtcatgg
     4021 cattactcgg tcccagcggc tgcgggaaat ccaccgtatt agcggtcatc gccggtttgg
     4081 agcaagccga tgccggtggg ataagctggg acggcaaatc cttgcgggag gtgcccccgc
     4141 acgagcgcgg ttttggtctg atgttccaag attatgcttt gttcccacat atgaacgtgt
     4201 ttggtaacat tgtctatggc ttgcggatga gaggctggcc tgaagatcgc ctgcgaaaac
     4261 gggtgggtga catgctggag ttggtgggtt taccaggttt cgataagcgt gatgtgaaca
     4321 ccctttcggg tggcgagcaa cagcgcgtgg cgctggcccg ttcgctcgct cctgagccgc
     4381 gccttcttat gctggatgag ccgcttggct cgctggatcg caatctgcgc gagcggttgg
     4441 tctcagattt gcgccaaata ttgaagagaa tgcatcaaac tgccatctac gttacccacg
     4501 atcaggaaga agcttttgct ttggcagatc gcgtggctgt gatgaacgcg gggcgcctgg
     4561 agcaacttga tgccccgcag atgatttatg cccgtccagc ttcgcccttc gtggcacgct
     4621 ttttgggaat gaccaacctg ctggatggcg tgatccgccc cacgttggat gggatcgtcg
     4681 tggagacggc ggttggcgcg ttccccgtcg ttggccaatt tcaaggcagg gttaccttgc
     4741 tcctgcgccc cgacgctgtg cgtccggatg gggtggggag tatgcatctc cagggcaggt
     4801 tgctcggttg ttccttccgc ggtagctcct gccaggcgac tatcgaagtg gatggcgttc
     4861 cattaaaatt tgattttctg actagcacgc ccttgccgcc tgccggcgaa ttagtgaccc
     4921 tgggatttga gcctggggat gcactgcaag tgttctcaca atgaagccaa gctcatacgc
     4981 tggtttgaga tagaataggg catcgcaact taactaatcc tacaattacg caagcgctct
     5041 tctcaatatg tcttcgatta cggtaattaa gctcgatatc aatggggtgg agacctggcg
     5101 ttatccaggt cagttactgc acaaacaaga cacgcaaatt gtcctggagg cgtattttga
     5161 ccgcgacgac atgatctttc atggaatgcc tcttcacaga ggtgatcgct tcctcgaatt
     5221 gtattttaca gaccgctggt ataacattta tgaaatccac agccgcgcag atgatcatct
     5281 gcgcggctgg tactgtaatg tgtgtacccc cgcccagcta gatggggaca ggttgttttt
     5341 caaagatctg gctctcgatt tgctggtctt ccccgatggg aaccagattg ttcttgatga
     5401 ggatgagttt gcccgccttg agctttctcc agacgggcgc agactggctt tggctgcttt
     5461 ggatgaactg cgagcgggtt tca
//
LOCUS       DBNHOH010000520         2329 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1442270, whole genome shotgun sequence.
ACCESSION   DBNHOH010000520 DBNHOH010000000
VERSION     DBNHOH010000520.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1442270"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1484)
                     /locus_tag="AC16V7_09100"
     CDS             complement(<1..1484)
                     /locus_tag="AC16V7_09100"
                     /inference="COORDINATES: protein motif:HMM:NF015557.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR6257318.1"
                     /translation="MTYEISALLFILGIAVVLFSFEWVSVDIVAIGILLSLVLLGLVP
                     TEDAFAGFGNETVVMILCLFILTAALVRTGVIDIIGRALLSRINNNPSQITIIVMGAA
                     AVMSTFMSNTASAAFFAPVVMGLSRRLRISASRLLMPVAFAAILASSMTLVSTSTNLV
                     VNGLMLQYGMKAMGMFELTPVGIPILLVGLVYMASIGKRMIPDRSHPEELTQEFNLRP
                     YLTEIVILPNSSLAGKTLVESGLGREYDFTVLRITRDDDVHLVPHADVLLEQGDVLLV
                     EGHRDQLFKIKDWVGIGVAEEVALSDPDLQTKDYQLAEVILLPRSPLIGRRLTGVDLR
                     EQYGVQVLAINRHESTLRSKIGQIPLQMGDVLLVQGPRFNLSTLEKSNTFRVLGTFEN
                     MQPNHSRAWLAMAIFVGALLLAIFDLVSLPVAVLLGTLVAFITRCITPEEAYREVEWK
                     AVILIGSMLSLGGAMEFTGTSKFLALQIVRLVGETNPVWLLTGF"
     gene            1778..>2329
                     /locus_tag="AC16V7_09105"
     CDS             1778..>2329
                     /locus_tag="AC16V7_09105"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6257319.1"
                     /translation="MLERAQHCCSFHTVTLIAMALDTSRIQALCFDVDGTLSDTDDQF
                     VLRLTRMLRPVSFLFPRRDPHSFARQVVMATESPGNYLLGLPDRLGFDHNLAALGDWI
                     YRIGLGRGKSRFLLVPGVRELLDRLQPRYPMAIVSARGARSTRAFLDQFELSHFFKCT
                     VTAHTCSHTKPYPDPIQFAAQEMG"
BASE COUNT          621 a          605 c          545 g          558 t
ORIGIN      
        1 aaaaagccag tcaacaacca gactggattc gtctctccga ccaagcgcac aatctgcaat
       61 gccaggaatt tggaagtgcc agtaaactcc atggcaccac caagagagag catacttcca
      121 atcaagatga ccgccttcca ttctacttca cggtaggctt cctcgggagt tatgcagcgt
      181 gttataaaag ccaccagtgt acccaggagc acagccactg gcaatgagac cagatcaaaa
      241 atcgcaagta acagtgcgcc gacaaagatc gccatggcta accatgcacg gctgtggttg
      301 ggctgcatgt tctcaaaggt tcccaggacg cgaaacgtat tggatttctc aagcgtggac
      361 aagttaaagc gcggaccttg taccagcagc acatctccca tttgcaatgg aatttggccg
      421 attttgctgc gcaaggtaga ctcatggcgg ttgatagcca agacctgtac accgtactgc
      481 tcacgcaaat cgacaccagt taatcggcga ccgatgagtg gtgagcgtgg taacaggatc
      541 acctcggcaa gttgataatc tttagtttgt aaatccggat cagaaagtgc tacttcttca
      601 gccacgccga tcccaaccca atcctttatc ttgaagagct gatcgcgatg gccttcaacg
      661 aggagcacat ccccttgttc caaaagtaca tccgcatgag gcaccagatg cacgtcatca
      721 tcgcgcgtga tgcgcaatac cgtgaaatca tactctcgac ccagaccaga ttcaaccaac
      781 gttttccccg ccagtgaaga attgggcaga atgacaattt cagttaggta agggcgcaaa
      841 ttgaattctt gagttagttc ttcaggatga ctccgatcag ggatcattct cttcccaatc
      901 gacgccatgt agaccaagcc aaccaggaga atgggaatcc ccactggggt tagctcgaac
      961 atgcccatcg ctttcatacc atactgcagc attagcccgt tgacaacgag gttcgttgaa
     1021 gtgctgacga gggtcatcga ggaagccaag atagctgcaa acgccacagg cataagcaat
     1081 cgggaggcgc tgattcgcaa ccttcttgat agccccatca caactggcgc aaagaatgca
     1141 gctgaagctg tgttactcat gaatgtactc atcactgctg cggcgcccat tacaattatg
     1201 gttatctggc tggggttatt gttgattcgg ctaagcaaag cgcgtccaat aatgtcgatg
     1261 acccccgtgc gtaccagggc agccgtcaag atgaacaaac acaagatcat aaccaccgtc
     1321 tcgttgccaa aaccagcaaa cgcatcctca gtaggcacca acccgagcag caccagtgac
     1381 agcaaaatgc ctatggcaac aatatcgaca gacacccatt caaaggaaaa caataccaca
     1441 gcgataccta aaatgaagag cagggcgcta atctcgtaag tcatgctaca cttgaggcaa
     1501 ttcttgagta cggctcaccg aatcaacttt ttgttacggt gcaatgggat tttctataaa
     1561 agagggaagt ttctctcgcc acgacaactt tattgatcag aacttgactt tgaaataata
     1621 caggctgatg tgataatatt gggattttac tataatcctc gtttagctgc gccacttgtt
     1681 ggctagacgt aattgtgaag attaagatct gaaaaatgtc tgaccttctc caaaaaatcg
     1741 gcatgtcttg cctgacgctg ttaattgcag cctgtttatg cttgagcgcg ctcagcattg
     1801 ctgcagcttt catactgtta cgttgattgc catggccttg gacacttccc gcattcaagc
     1861 tctttgtttc gacgtagatg gcacattgag tgatactgac gaccagtttg tgttacggct
     1921 gactcgcatg cttcgcccag tgagcttttt attccccagg agagatccac attccttcgc
     1981 caggcaagtg gtaatggcaa cagaaagtcc tggtaactac ctgcttgggt tgcccgatcg
     2041 tttggggttt gaccataatt tggcagccct tggcgattgg atatatcgca tcggcttagg
     2101 aagagggaaa agccgattct tgcttgtacc gggcgttcgc gaattgctcg atcgtcttca
     2161 gccccgctac ccgatggcga tcgtcagcgc gcgtggagct cggagcacgc gcgcctttct
     2221 agatcaattt gaactctccc atttttttaa atgcactgtg actgcgcata cctgttcgca
     2281 caccaagccg tacccagacc cgatccaatt tgccgcccaa gagatgggg
//
LOCUS       DBNHOH010000521         2997 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1762026, whole genome shotgun sequence.
ACCESSION   DBNHOH010000521 DBNHOH010000000
VERSION     DBNHOH010000521.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1762026"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="AC16V7_09110"
     CDS             <1..323
                     /locus_tag="AC16V7_09110"
                     /inference="COORDINATES: protein motif:HMM:NF017828.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HIR6257320.1"
                     /translation="SLAEGTLLSVRDAMDSMFHQNLALEEMIDEYSIDGVIFHPIKSC
                     RTVSTGLADGRRALMAARDIPSLFIESDMMDRRVVSEAQMKNRIDAFFEGLASRRQRE
                     TVTV"
     gene            340..1143
                     /locus_tag="AC16V7_09115"
     CDS             340..1143
                     /locus_tag="AC16V7_09115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HIR6257321.1"
                     /translation="MAYAAGVDVGSTQTKAIIVNENEEIVSRALVDTGANVVQAAENA
                     FREVLISGDVREEEVEFVVGTGYGRYRVTFGNTQVTEISCHARGAIKMFPNTHTVIDM
                     GGQDTKAIRVSPTGEVLDFCMNDKCAAGTGRFLGAASMALEIPLDNLGPTALLAEKPV
                     RISTTCTVFAESEVLSWLGKGKKIEDILLGVHQSIASRSVGLLRRVGIEEEITFTGGV
                     AKNVGMIKALEDNLEMKLNVSPDSHYMGALGAALFALDHIISKRAPLTA"
     gene            1157..2002
                     /locus_tag="AC16V7_09120"
     CDS             1157..2002
                     /locus_tag="AC16V7_09120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HIR6257322.1"
                     /translation="MSYVTGIDVGSTYTKAVILDENNEMVGRGMISTSFKLAEAARRA
                     YQLALEQAGLQEADIAYIVATGFGRHQIPFRDVNVTDLTASSFGASFLYPKTSTILDV
                     GGQTMKAIRLDGNQRVKSFRLNDKCAAGTGAFLEKTARYLGYSTEEIGTLAATSKTAV
                     PISGVCAVFAESEVINHLSQGAAPADIMYGSIISLVDRSVQLMKRVRMEPEFTLIGGI
                     LRFSSMAKVVREQLNTEVNVPEGDLVQFTTALGAAILGQRRLKKLQEAKADQETIEDK
                     SLAET"
     gene            2007..2372
                     /locus_tag="AC16V7_09125"
     CDS             2007..2372
                     /locus_tag="AC16V7_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257323.1"
                     /translation="MPDIQQGNLQDEVDISKTSPAPKAQPQLDETANCYPQTPVSKHD
                     LSLPDLDFEPDPRLVAEGWERRFMADADRAKEAVELYQSLGFEVLTEEVKPTELSDLC
                     LDCQLVVCRYYVTVYTRKK"
     gene            2419..>2997
                     /locus_tag="AC16V7_09130"
     CDS             2419..>2997
                     /locus_tag="AC16V7_09130"
                     /inference="COORDINATES: protein motif:HMM:NF044910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaX family transcriptional regulator"
                     /protein_id="HIR6257324.1"
                     /translation="MNFSERPAVRTQFLVFTLFGEYVLPRGGSIWTSNLLSLMEVLGV
                     SERAARSTLSRMSRKGWITARKEGRRSRYSLTPRGWMLLKQGEKRIFEPTPQEWDGLW
                     YLVVYSLPEKKRRLRHSLRQGLSWLGFGPLSPGTWVSPHNRFIEVKNMCKDLGVQQHV
                     EIFSGAHVGLSTDQEIVRTCWDLAELEAQYKEF"
BASE COUNT          733 a          752 c          840 g          672 t
ORIGIN      
        1 agagcctggc agaaggcaca ctgctcagtg tgcgagatgc aatggacagc atgttccacc
       61 aaaatttagc cttggaggag atgattgacg agtatagcat cgatggcgtc attttccatc
      121 ccatcaagag ctgccgcact gtatcgactg gcctagctga tgggcggcgg gcgttgatgg
      181 ctgcccgaga tatccccagc ctgttcatcg agtcggatat gatggaccgc cgggtggtat
      241 ctgaagccca aatgaagaac cgcattgatg ccttttttga aggtttggca tctcgccgac
      301 aaagagaaac ggtgactgtt tgagtcagga gagggttcca tggcatatgc tgcaggtgta
      361 gatgttggtt ccacacagac caaagcgatc atagtcaatg aaaacgaaga gatcgtcagt
      421 cgcgccctcg tagatacggg cgccaatgtc gttcaggctg ctgaaaacgc cttccgggaa
      481 gtgcttatct ccggtgatgt gcgtgaagag gaggtagagt ttgttgttgg tacaggttac
      541 ggacgctacc gagtaacctt tggcaatacg caggtgacag agatcagctg ccacgcacgc
      601 ggggcaataa aaatgttccc caacacccac accgtgatcg atatgggtgg gcaagataca
      661 aaggctattc gcgtcagccc gaccggcgaa gtcttggact tctgcatgaa cgacaaatgc
      721 gcggctggca ccggacggtt ccttggggcg gcatccatgg cactggagat ccccctggac
      781 aacctaggac ccactgcctt gctagctgaa aaaccggtcc ggatcagcac aacctgcacc
      841 gtttttgctg aatcggaagt gctttcctgg ttaggcaaag gcaaaaaaat cgaagatatc
      901 ctgttgggag tgcatcagtc gattgcctcg cgctcggtcg gattgctgcg ccgggtcggg
      961 attgaagaag agatcacctt cactggcggg gtggcgaaaa acgtaggcat gatcaaagcc
     1021 ctggaggata acctggagat gaaattgaat gtcagcccgg attcgcatta tatgggtgct
     1081 ttgggcgcgg ccctctttgc actagatcat atcatttcaa aacgagcgcc tctcaccgca
     1141 taaggcagga gcaaccatga gctacgttac cggtatcgac gttggatcca cgtataccaa
     1201 agccgtcatc ctggatgaaa acaatgagat ggtcggtcga gggatgataa gtaccagctt
     1261 caagttggcg gaggcggcgc ggcgcgcata tcagctcgca ttggagcaag caggcctgca
     1321 ggaagccgat attgcctata ttgttgccac gggttttggt cgtcaccaaa ttccttttcg
     1381 cgacgtgaac gtaaccgacc tcaccgccag ctccttcggg gcatccttcc tctatcccaa
     1441 gacgtccacc atcctggatg taggtgggca gaccatgaaa gccatccggc tggacgggaa
     1501 tcagcgcgtg aagtccttcc gcctgaatga caaatgcgca gctggtacgg gtgcctttct
     1561 ggaaaaaaca gcccgctatc taggttactc caccgaggag attggcacac tggcagcgac
     1621 ttcaaaaacg gctgtaccca tctctggggt ttgcgcagtg ttcgccgaat cagaggtcat
     1681 caaccatctc tctcaaggcg cggcgccggc cgacatcatg tatggatcta tcatttcgtt
     1741 ggtcgaccgg tctgtacagt tgatgaagcg tgtccgcatg gaacccgagt tcaccctgat
     1801 tggcggaata ctgcgcttca gctccatggc taaagtcgtg cgggagcagt tgaacactga
     1861 agtaaacgtg cctgagggtg acctggtcca atttactacg gcgctgggtg ctgcgattct
     1921 cggccaacgc cggttgaaga aactgcaaga agcaaaggct gaccaagaga cgatcgaaga
     1981 taaatcgcta gctgagacat aacgatatgc ctgatatcca acagggtaat ctccaggacg
     2041 aggttgatat ctcgaaaacc agcccggctc ccaaggccca gccccaacta gatgaaacag
     2101 ccaactgtta cccccaaacg ccagtaagca aacatgatct ttctttgccg gatctcgatt
     2161 ttgaaccaga cccgaggctg gtagcagaag ggtgggagcg gcgttttatg gccgatgcgg
     2221 atagagcgaa agaggctgtt gagctatacc agtcgcttgg ttttgaggtc ttgacggaag
     2281 aggtaaaacc aacggaattg agcgatttgt gcttagactg tcaactggtt gtctgccgtt
     2341 actacgttac cgtgtacact cgcaagaaat gacccctatt ctcttaaata aggatgctga
     2401 ccgaggatat gtaggttgat gaacttctct gaacgcccgg ctgtaagaac ccaattcctc
     2461 gtttttacct tatttggtga gtatgtctta ccgcgcgggg gttctatatg gaccagcaat
     2521 ctgcttagcc tgatggaggt tctgggggtc agcgagcgtg ctgcccgttc cactctttca
     2581 cgcatgtccc gcaagggctg gatcactgcc agaaaagagg ggcgacgcag ccgatattca
     2641 ctaactccac gcggctggat gctgttgaaa caaggtgaaa aacgcatttt cgaacccact
     2701 ccccaggaat gggatggtct atggtacctg gtggtgtatt ccctgcctga gaaaaaacgc
     2761 cggctgcgcc actctttgcg ccaaggcttg agctggttgg gcttcgggcc actatcaccg
     2821 ggaacctggg tatctcctca caatcgtttc atcgaagtaa agaatatgtg caaagacctg
     2881 ggggttcagc agcacgtgga aattttttca ggcgcacacg tcggcctgtc tacagaccag
     2941 gaaatcgtgc gcacatgctg ggacttggct gaattggaag cgcaatataa ggaattt
//
LOCUS       DBNHOH010000522         5217 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_672310, whole genome shotgun sequence.
ACCESSION   DBNHOH010000522 DBNHOH010000000
VERSION     DBNHOH010000522.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5217)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_672310"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /gene="dnaA"
                     /locus_tag="AC16V7_09135"
     CDS             <1..918
                     /gene="dnaA"
                     /locus_tag="AC16V7_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIR6257325.1"
                     /translation="NPLFLYGGVGLGKTHLLHAIGNACAERGTRILYVSSEEFTNDLI
                     NAIRTHTTQAFREKYRQPDVLLIDDIQFIAGKESTQEEFFHTFNALHSQDKQLVMTSD
                     RPPKAMVTLEERLRSRFEWGLIADIQPPDYETRLAILRSKADRAGYRMQSEILELIAR
                     RIQSNIRELEGALTRIAAFAELSGMPMTAQLAESVLTDLLPRRNEVQPEEVIRKVADV
                     FGVAFERMLGRDRSREVALPRQIAMYLLREEANISLPQIGQTLGGRDHTTVMYGCDKV
                     ADLLEQDDRLRRQVIQIKEQLFGMSQLGY"
     gene            complement(1016..2227)
                     /locus_tag="AC16V7_09140"
     CDS             complement(1016..2227)
                     /locus_tag="AC16V7_09140"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR6257326.1"
                     /translation="MSEQTPANPSPNWGSLTKLVVGLTFIAIIAALLVQFRSIVGPIL
                     LAFILAYLLYPVAAMLSKVSRLSWRASVNLIFLLVVVIYLGLITLTGFTVVQQLQSLI
                     DFVRRAVTDLPSLAERLSTQVYQFGPWQFSTGQLDLEALSRELLSTLQPLLGRMGSLI
                     STFATSAAATLGWSFFVLIIAYFLLADTGQFPNQLLHIGIPGYDDDISHLGIELRKIW
                     NAYLRGQLIVILLVMISYSILLVILGMRFAFGIAILAGLARFVPYVGPLTTTVVTFLV
                     ALFQGENYFGLLPYQYAILVVVASFILDQIYDNLISPRLLGEALGVHPAGVLIVAIIA
                     ANLIGLIGLVLAAPVLATLRLISRYVGRKMFDLNPWPETEPPPPTSPLDVPWSRWIRR
                     LRAWARSLKLK"
     gene            complement(2224..3396)
                     /locus_tag="AC16V7_09145"
     CDS             complement(2224..3396)
                     /locus_tag="AC16V7_09145"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR6257327.1"
                     /translation="MQETHPRWSMQAKLTISFLLIALAIFLLYRFRVILAPMIMALIL
                     AYVLSPLVNALQNRLGIRRAIATLITYLILLAVLVVLPLVIIPPLVTQITGLNLNIQR
                     FLLEIENILGRRFMLAGQMIDLDQLIQQAIGSLQGFMEPVFGQTLSLAVEVISSVVWV
                     IFILVVVFYLIKDGPAMREWLEKITPPAYRNDYIRLRDEISSIWAAFFRGQLVLAVVV
                     SVIFTVIGFILGLPFAFAMGVFAGLMEFLPSIGHGIWLLVASLIALFGGSTWLPLPNL
                     AFMLIVIGLHLVYQQFDLNYLIPRIIGRSVHLPPLVVILGIVTGAVLAGVLGILLAAP
                     TIASARVLGRYIFANLFDLEPFPVSEAPPASQPNLRWWRKLLPRQRADLEQESEDA"
     gene            complement(3582..3797)
                     /locus_tag="AC16V7_09150"
     CDS             complement(3582..3797)
                     /locus_tag="AC16V7_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257328.1"
                     /translation="MGKKSRTSRRSSRAVSSSATPSKPNGITTTVRPFERDFSPDYAP
                     VIMDLKRIGVLAGTFFVLLVVLSFFLK"
     gene            complement(3815..4720)
                     /locus_tag="AC16V7_09155"
     CDS             complement(3815..4720)
                     /locus_tag="AC16V7_09155"
                     /inference="COORDINATES: protein motif:HMM:NF016119.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HIR6257329.1"
                     /translation="MARKRTPEELSVEELRWLMVEKRRTVRQERLERFRRTGRALVIA
                     PDVDNSLEQMHTGVLEDETDEAKPPRSRARRALDGFLLLLEISAVAGFLFVLFNGLRL
                     IQDLNKEVAAALVQPTLTPTPLIVAVVLPSGHTPPTTAGGARPNDAEIPEHLRPLVQS
                     LASVPIPTPGPEQAIRIQIPAINVDAPVVQGDGWEQLKKGVGQHLPSTNPGKEGNMVL
                     SAHNDVFGEIFRDLDQLKPGDEITIFSNQRAYTYIVAQSQVVEPTHVEVMAPTKEPTV
                     TLISCYPYLVDNQRIVITAQLQGGN"
     gene            4824..4913
                     /locus_tag="AC16V7_09160"
     tRNA            4824..4913
                     /locus_tag="AC16V7_09160"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4860..4862,aa:Ser,seq:gga)
BASE COUNT         1220 a         1397 c         1523 g         1077 t
ORIGIN      
        1 aatccgctgt tcctgtatgg cggcgtcggg ctgggcaaaa ctcacctgct ccacgcaatc
       61 gggaacgcct gtgccgaacg cggcactcgc atcctgtatg tctcttccga agagttcacc
      121 aacgacctga tcaacgccat ccgtacccat acgactcagg catttcgaga gaagtatcgc
      181 caacccgatg tactgctgat cgatgatatc cagttcattg caggcaagga atcgacccag
      241 gaggagttct ttcatacctt caatgccttg cacagccagg acaaacaact ggtcatgacc
      301 tcggaccggc ctccgaaagc gatggtgacc ttggaagagc gcctgcgctc acgcttcgaa
      361 tggggactga tcgctgatat ccagccgcct gattacgaaa cccgtttggc tattttgcgc
      421 tcgaaggcgg atcgagcggg ataccgcatg cagagcgaaa ttttagagct tatcgctcgt
      481 cgcattcagt cgaatatccg cgagctggag ggggctctta cccgcatcgc tgcctttgct
      541 gagctgagcg gcatgcccat gacagcgcaa ctcgccgaat cggtgctgac agacctcctg
      601 ccacgccgta acgaagtgca gcctgaagag gtaatacgca aagtagctga cgtattcggt
      661 gtggcctttg agcggatgct ggggcgcgat cgctctcgcg aggtggcctt accacgccag
      721 attgccatgt accttttacg cgaggaggcc aatatctcct tacctcagat tggccaaacc
      781 ctgggtgggc gcgaccatac gaccgtcatg tacggctgtg acaaggtagc cgacttgctg
      841 gaacaggatg atcgtctgcg caggcaggtg atccagatca aggagcagtt attcggcatg
      901 tcgcaattgg gatattaaaa ccgactagct cattggggta cgtgttggct tacccaaaga
      961 gctagatgat taaaaatgga tcctgggtag tttgccttcc ctacccaggg tctatttatt
     1021 tcaatttcag tgagcgtgcc caggcgcgca ggcgccttat ccagcgcgac cagggcacat
     1081 ccagagggct ggttgggggc ggaggctccg tctcaggcca aggattcaga tcaaacattt
     1141 ttcggcccac atatcggctt ataaggcgca gggtagcgag gacgggcgct gccagcacca
     1201 acccaatcag cccaatcagg ttggcagcga tgatggcaac aattaataca cctgccgggt
     1261 gcacccccag ggcttcaccc agcaagcgcg gcgagatcaa gttatcgtag atctggtcga
     1321 ggataaatga tgctaccacg accagtatgg catattggta cgggagcaag ccgaaatagt
     1381 tctcgccctg gaataatgcc accaggaatg tcaccaccgt ggtggttagc ggaccgacgt
     1441 agggcacaaa gcgggccaaa ccagctagga tggcgatacc gaaagcgaag cgcatgccca
     1501 ggatcaccag caagatggaa taggatatca tcaccagcag gatgacgatc agctggccgc
     1561 gcaggtaggc attccaaatc ttgcgcagtt cgataccgag gtggctaatg tcgtcatcgt
     1621 agccagggat gccaatgtgc aacaattggt tggggaattg accggtgtct gccagtaaaa
     1681 agtaggcgat gatcagcacg aagaagctcc accctagtgt ggcggccgcg ctggtagcaa
     1741 aggtgctgat caggctgccc attcggccca gcaggggctg caaggtggag agcagctccc
     1801 ggcttaaggc ttccaggtca agctgtcccg tactgaattg ccagggtcca aactggtaga
     1861 cttgggtgga taggcgctct gctaaagagg ggaggtcggt caccgccctg cgcacaaaat
     1921 cgatcaggct ctgcaattgt tggacgacag taaagccagt gagcgtgatc agacccaggt
     1981 agatcaccac caccagcaag aagatcaggt tcaccgaagc tcgccatgag agccggctga
     2041 ctttgcttag catagctgcc accggatata gcaggtatgc caggataaat gccagtaaga
     2101 ttggcccgac gattgagcga aattgcacca aaagcgcagc gatgatggcg atgaatgtca
     2161 ggccgaccac cagtttggtc aacgaccccc aattgggaga aggattggca ggtgtctgtt
     2221 cgctcatgcg tcttccgatt cttgttcgag atccgcacgc tggcgaggca acagcttgcg
     2281 ccaccagcgc aggtttggtt gggatgcagg gggagcttca ctaacgggaa acggctccag
     2341 gtcgaacaag ttggcgaata tatagcgtcc cagcacccgg gctgaggcga tggtgggcgc
     2401 tgccaacaag atgcccagca cacctgccag caccgcgccg gtgacaatcc ccaggatgac
     2461 gaccagcggg ggcaggtgca cgctgcgccc gataatgcgc gggatcaggt agtttaaatc
     2521 gaattgctgg taaaccaggt gcaagccgat cacaatcagc ataaacgcca ggttgggtag
     2581 tggcagccag gtggagccgc cgaatagggc gatgagcgag gctaccaaca gccagatgcc
     2641 atgcccgatg ctgggtagaa actccatcag cccggcaaat acccccatgg cgaaggcgaa
     2701 tggcaaaccc aggataaagc cgatgacggt gaagatcacg ctgacgacca ccgccagcac
     2761 caattggcct ctgaagaaag ctgcccaaat cgagctgatc tcgtctctca ggcgtatgta
     2821 gtcgttgcgg taggctgggg gggtgatctt ctcgagccat tctctcatgg caggaccatc
     2881 tttaatcagg tagaacacaa ccaccaggat gaagatgacc cacaccaccg acgagatcac
     2941 ttcgactgcc aggctgagcg tttgcccgaa gaccggctcc atgaaaccct gcaaagagcc
     3001 aattgcttgt tggatgagct gatccaggtc tatcatttga cctgccagca tgaatcggcg
     3061 gcctaatatg ttctcgattt ccagcaggaa gcgctggatg ttcaaattca acccggtgat
     3121 ttgggtgacc agcggcggga tgatgacgag tggtagcacc acgagaaccg caagcaggat
     3181 caggtaggta atcagcgtcg ctatagcacg ccggatgccc aagcgatttt gcagcgcatt
     3241 gaccagtggc gataaaacat acgccaggat gagcgccata atcattggcg caagaatcac
     3301 cctgaatcta taaaggagaa agattgccag agcgatgagc agaaagctga tggtcagctt
     3361 ggcttgcata gaccagcgcg ggtgtgtctc ttgcatgttc acattgtatt ataaaatagc
     3421 ggattttgga tgagggtcaa cgcgctaagg caaactgtct ctaatgggct taaatccaga
     3481 gattgggata tgcagctaca tatcccaatc tctggatttt ttccttcact ttatggtcag
     3541 cggggaggtg acgtgcacta ggttcgaaat aaattatgct tttatttcaa gaagaaggaa
     3601 agcacgacga gaaggacgaa gaaggtccct gccaggacgc cgatgcgctt cagatccatg
     3661 atcaccggtg cgtaatcagg gctgaaatcc cgctcgaagg gccggacggt ggtggtgatg
     3721 ccgttgggct tgctgggggt cgcagaagat gaaaccgctc tggacgatct ccgggatgtt
     3781 ctagatttct tacccataaa aacctcgctt tcttttaatt tccgccctgc agttgggcag
     3841 tgatgacgat tcgctggttg tcaaccagat aaggataaca cgaaatcaac gtaaccgtgg
     3901 gctctttggt gggtgccatg acctcaacat gggtcggctc gaccacctgg ctctgcgcca
     3961 caatgtaggt gtaggcgcgc tggttgctga agattgtgat ctcgtcgccc ggcttgagct
     4021 gatccaggtc acggaagatc tcgccgaaaa catcgttatg ggccgatagc accatattgc
     4081 cctccttgcc aggattggtt gaggggaggt gctgacccac gcctttcttc aattgctccc
     4141 atccgtcgcc ctgcacgacc ggcgcatcca cattgatggc cgggatctgg atgcgaatgg
     4201 cctgctctgg tccaggtgtg ggtatgggca cactggcaag ggattgcacg agcggccgca
     4261 ggtgctctgg gatctcggca tcgttggggc gcgcccctcc cgctgtggtg ggtggtgtat
     4321 gcccggaagg caacacgaca gccacaatca atggggtagg ggtcagcgtt ggttgcacca
     4381 aggccgccgc cacctcttta ttcaggtcct ggatcaggcg caagccgttg aagagcacaa
     4441 agagaaagcc tgccaccgcg ctgatctcca acaggagcag gaaaccatcc agggcacgcc
     4501 gggcgcgcga gcgtggcggt ttggcctcat cagtctcgtc ttccagaacg ccggtatgca
     4561 tctgctcgag cgaattatcc acatcgggag cgataaccag cgcccgcccg gtgcgccgga
     4621 atcgctccag gcgctcctgg cggacggtgc ggcgcttctc gaccatcagc cagcgcagtt
     4681 cttcaacgga taattcctcg ggggtgcgtt tgcgggccac ggcgtgatta taccaacagt
     4741 tgctctctat ggtataatct cgcccgccag ggcagattat tttatgccgc aaacactgcg
     4801 ggcagagttg cctggcgctt attggagagg tcgcgtagtc tggcctagcg cgcacgcctg
     4861 gagagcgtgt ttcccgcaag ggaacgaggg gtcaaatccc tccctctccg ccagaagatt
     4921 tacgattgct gattttagat ttggggttgg caagcaaaga agaattgctc agcatgtgct
     4981 gggcgatttc ttttaatgga ttttcgttgg atagagcagt gcggatggtt acggacagca
     5041 agcggcaccc cgcatgcatt cctccggggc tatgctccag cctgcacccc gctccccagg
     5101 tgccctacgg ggtcaccggc acggatgggg caaggtattc aattgttgac cacccccatg
     5161 ttcactttag ggtcgcgcta gccccttata taaccacgtg tctaccggac ggggagc
//
LOCUS       DBNHOH010000523         2869 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_287671, whole genome shotgun sequence.
ACCESSION   DBNHOH010000523 DBNHOH010000000
VERSION     DBNHOH010000523.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_287671"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1238
                     /gene="atpD"
                     /locus_tag="AC16V7_09165"
     CDS             <1..1238
                     /gene="atpD"
                     /locus_tag="AC16V7_09165"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR6257330.1"
                     /translation="CLAMDVTDGLQRGVAAYATGAPIKVPVGLETLGRVFNVLGQPVD
                     NLGDVEAKQYYPIHRPAPEFADQSTRVEVFETGLKVIDLIAPFTKGGKTGIFGGAGVG
                     KTVIIMELIRSIATVHQGNSVFAGVGERTREGTQLYREMNESGVMKDTVMVFGQMNEP
                     PGVRLRIALAALSMAEYFRDQGRDVLLFIDNIFRFSMSGSEVSALLGRMPSAVGYQPT
                     LATEMGELQERITSTRHGSITSMQAVYVPADDYSDPAPVATFTHLDATIALERSISEK
                     GIYPAVDPLASTSRILDPSLVGEDHYTTAREVQRVLQRYKDLQDIIAILGVDELSEDD
                     KLIVSRARKIERFFSQPMFVAAQFTGLEGRYVPLRDSVRGFREILDGKHDDLPEQAFM
                     MVGTIEEAREKAERLSQSS"
     gene            1248..1700
                     /gene="atpC"
                     /locus_tag="AC16V7_09170"
     CDS             1248..1700
                     /gene="atpC"
                     /locus_tag="AC16V7_09170"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HIR6257331.1"
                     /translation="MPIRCEIVSQDRLVFEGDADIVVVPGAMGEMGILPNHAPLLSTL
                     KFGILKVRYQGQEQVFAVAGGVVEIQPDIITVLADAAENVQEIDEARAEEARKRAEEY
                     LKAGPPPDTDAYLAMEAALRRSNLRLEAVQRYRRGQSVRQVQSGEGET"
     gene            1707..2714
                     /gene="mreB"
                     /locus_tag="AC16V7_09175"
     CDS             1707..2714
                     /gene="mreB"
                     /locus_tag="AC16V7_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HIR6257332.1"
                     /translation="MALLSRELGVDLGTMNTRVAEGGRVVLEEPTVAAIVVQEQKMVA
                     WGKEAMDMFGRVPESMEVTRPMQNGVIADYEVTEYMLQALLRRLSAPLRFFRPTIMVT
                     VPYGVTSVESRAVHEASLQAGSREAYLIQQPLAAAIGVDLPIGTPSGNMIVCLGGGAT
                     QAAVLAMYGIVSAETLRAGGMSLDEAIVSYIRKKYGLIIGQLTAEQVKIQIGAAVPQD
                     EESSFEIQGQDQVNGLPRPATLTTGEVVDAMRDPLNDIIETARRVLEKTPPELVSDII
                     DRGIALTGGGALLRGIDKLFTKQLGVPAYLVDNPTTCVAEGASRGLSIYPLIRRNLPP
                     V"
BASE COUNT          664 a          691 c          818 g          696 t
ORIGIN      
        1 gttgcctggc aatggacgta accgatggcc ttcaacgagg tgtcgccgcc tatgccactg
       61 gcgcaccgat caaagtgcct gtagggctcg aaacgcttgg gcgcgtcttt aatgtacttg
      121 gtcaacctgt cgataatctg ggcgacgtgg aggctaagca atattatccc atccatcgcc
      181 ccgcacccga atttgctgat caatctacac gagtcgaggt gttcgagacc ggtttgaagg
      241 ttattgactt gattgccccg tttactaaag gtggtaagac aggtatcttt ggcggcgctg
      301 gcgtgggcaa aaccgtcatt attatggaat tgatccgttc aattgctaca gtacaccagg
      361 gcaactccgt ttttgctggt gtcggtgagc gcacgcgcga gggtacccag ctctaccgag
      421 agatgaacga atctggcgtt atgaaagata ccgtaatggt atttggccag atgaacgagc
      481 ctccgggggt gcgcctgcgc attgccctgg cggcgctatc catggcggag tactttcgtg
      541 atcagggacg ggatgtgctg ctgtttattg ataatatttt ccgctttagc atgtcgggct
      601 cggaggtatc cgcattgtta gggcgtatgc cctctgcggt gggttaccaa cccactctgg
      661 caaccgagat gggtgagctg caagagcgta tcacctctac acgccatggc tcgatcacgt
      721 cgatgcaagc agtgtatgtg cccgccgatg actattctga cccggctccc gttgctacat
      781 tcactcacct agacgccacc attgccttgg agcgatcgat ctctgagaaa ggcatttacc
      841 ctgcggtaga tccgcttgca tcaacatccc gcatccttga tccaagtctg gttggtgaag
      901 atcattacac gacagcacgt gaagtacaac gagtgctgca acgttataaa gaccttcagg
      961 acatcatcgc catcttgggc gtagacgagc taagcgaaga tgacaagttg attgtttcaa
     1021 gggctcgtaa gattgagcgt ttcttctcgc aacccatgtt tgtcgctgcc cagttcaccg
     1081 gacttgaagg ccggtatgtg ccactaagag acagtgtgcg tggcttccgt gaaatcttgg
     1141 atggcaagca tgatgaccta cctgagcaag cttttatgat ggtaggaacc atcgaagaag
     1201 cgcgcgagaa ggctgagcgt ttatctcagt ccagctagag gaataagatg cctattcgct
     1261 gtgagatcgt ctctcaagat cgcctggttt tcgaagggga tgcggatatc gttgtagtac
     1321 caggcgccat gggggagatg ggtatactgc cgaatcatgc acccttgcta tctacactga
     1381 aatttggaat cctcaaggta cgatatcagg ggcaggaaca ggttttcgcg gttgctgggg
     1441 gtgtggtgga aatacaacct gatatcatca ccgtgttggc agacgctgca gagaatgtgc
     1501 aagagattga tgaggcgcgg gctgaagaag cccgtaaacg cgctgaagag tatctaaaag
     1561 ctggtccacc ccctgatacc gatgcctacc tggctatgga ggccgcgctg cgccggtcca
     1621 atttgcgcct ggaggcagtg caacgctacc gccgaggtca atcagttcgt caggtccaat
     1681 ctggagaggg agaaacctaa tatattatgg ctctcctgtc gagagaacta ggcgtcgacc
     1741 tgggcaccat gaatacccgt gtggccgagg gtggacgcgt tgtgctcgaa gaacccacgg
     1801 ttgcagcgat tgtagtccaa gaacagaaga tggtggcttg gggtaaagag gcaatggaca
     1861 tgtttggtcg tgttccggaa tctatggagg tgacacggcc aatgcaaaat ggtgtgatcg
     1921 cggactacga ggtcactgag tacatgttac aagccttgct gcgccgcttg agcgcacccc
     1981 tgcgtttttt tcgccctacc atcatggtta ctgtacctta tggggtgacc agcgtggaga
     2041 gtcgagcggt acacgaggca tctcttcagg caggcagccg ggaagcttac cttatccagc
     2101 agcctttggc tgcagccatt ggggtggatc tgcctatcgg caccccatct ggtaatatga
     2161 ttgtctgtct gggaggggga gccacccagg cagctgtgtt ggcgatgtac ggtatcgttt
     2221 ccgcagaaac gcttcgtgcg ggtggaatgt cgctagacga ggcgattgtg tcgtatattc
     2281 gcaagaaata tgggctgatc attggacagc tcactgctga acaggtgaaa attcaaattg
     2341 gagcagccgt accccaggac gaagaaagca gttttgaaat tcagggacag gatcaggtaa
     2401 atggactgcc tcgcccagct acacttacga ctggtgaggt cgttgatgcc atgcgcgatc
     2461 cgctcaatga tatcattgag acagcaaggc gggttttgga gaaaaccccc cctgagttgg
     2521 tatcagacat cattgaccgc gggatagccc tcaccggagg tggtgcgctg ctgcgtggta
     2581 ttgataaatt gttcacaaaa caactgggcg ttcctgctta cttggtggat aatcccacga
     2641 catgcgtagc agaaggtgca tcccgtggat taagcattta ccccctcatt cgccgcaatt
     2701 tacctcctgt gtaggtttat aagcttacat taacgaaaaa gagagtagcc gatccggcta
     2761 ctctcttgct tttcaaacgg agatttattc caagtaataa gtcatacgtt gcaggtgcgt
     2821 caccggatcg gtgtgttgaa cgtggatccc ttcgggaacg ttaatattg
//
LOCUS       DBNHOH010000524         2700 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_608320, whole genome shotgun sequence.
ACCESSION   DBNHOH010000524 DBNHOH010000000
VERSION     DBNHOH010000524.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_608320"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..>2700)
                     /locus_tag="AC16V7_09180"
     CDS             complement(237..>2700)
                     /locus_tag="AC16V7_09180"
                     /inference="COORDINATES: protein motif:HMM:NF046269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MGH1-like glycoside hydrolase domain-containing
                     protein"
                     /protein_id="HIR6257333.1"
                     /translation="SSVVGNRPNAIALIRLTFRATYHTIQCTMRKWNLKAGDPLALTL
                     MADARLGPTDYCDDQIWELRIGGGDPPALALQTTFGLRARSVRVFPRFGEGDTMVTAP
                     AEFAAPPVVTNFFPNYLSVQCSPLSDIDVELEYWVPQSHAVAGRVGLMNNSSKARLIR
                     LSLVAQLNPTEGQRMAPIEMQNAPALAGMTGGLTPVMFVTGGPQAVGSPYPALNLEIE
                     LPPRASRRFTWCHAALQDVETSFSLARQMVACPWEAEIARVELVNSAQLEIYTGDPDW
                     DAVFAFSQKLAAATFVGPTQHLPFPSIVLSRQPDQGYSLRGDGGDYNHLWNGQPPLEI
                     YYLAGLLLPGGTSLLQGLVRNYIAVREEDGGLDWKPGLAGQRSGLLAMPILATLSWEI
                     FQISQDRVFLEDVFEPLLKFIYRWFDDRHDRDGDGVPEWDHPMQAGFEDHPVYSRWHE
                     WSQGVDISSAESPALCALLHRECKALIQMGQILERVESLPVLQSYADHLHTALDAAWA
                     PRRHSYLNWDRDSHYSTRGEKLGEITGPGEVLIQRQFDHPVRLFVRIKTADENTRHPD
                     LFIYGESASGQPRVERVPNEMFQWFLGQGSLTGERVYAKVERVKVQGIDQNDRMSLFS
                     VGYSRPDHTVLLPLWSGIPDSKRALRIVGKTILAERRYWRPYGIPACPDDAPNGHDQV
                     CRCAHLPWNNLIGEGLLGYGYQEEATELISRLMAAIIRNLKQNGAFSRYYHVETGEGL
                     GERNVLTGLAPLNLFLSTLGVRIYSHEKVFLSGSNPFPWPVTVKYRGLTVLRRADSTV
                     ITFPDGQTATITDPSPQMVTLD"
BASE COUNT          661 a          754 c          681 g          604 t
ORIGIN      
        1 ctgtgctggt aagctgcgcc accaataaac ccgctgcatt cagagctcgg ctagccttcc
       61 ggacgtcctg cgcctgaaca acggcaaaca ttaggcgatc cacagacggc ttaattcttt
      121 cagctccgcg ttccatgtcc ggcaacctta ccacctttag atacttttcg ttcatagatg
      181 tcacctgtat tatctatgca atcaaatatg ccaagatttt tttgatctca aaccagttag
      241 tccagggtaa ccatctgcgg agacgggtcc gtaatcgtgg ctgtttgacc atcaggaaag
      301 gtaatcaccg tgctatctgc ccgtcgcagc actgtcaacc ctcgatattt taccgtaact
      361 ggccagggaa atgggttaga accggataga aagactttct cgtgagaata tatgcgcaca
      421 cccagggtgc ttaagaaaag attcaacggg gcaagtccag tcaatacgtt acgctcgccc
      481 aacccttccc cggtttcaac atggtaataa cgtgagaatg caccattctg tttgaggtta
      541 cggataattg ctgccatcag acgcgatata agctcagtag cctcttcctg atagccatag
      601 cccagcaaac cttctccgat taaattattc cacggcaaat gcgcacagcg gcagacctgg
      661 tcgtgaccat ttggcgcgtc atctgggcag gctggaatgc catatggtcg ccagtaacgc
      721 cgttcggcca atatggtctt tcccactatt ctcagagcac gcttcgaatc gggtataccc
      781 gaccataagg gtaatagcac agtgtggtct gggcgagaat agcccacact gaagagagac
      841 atccggtcat tttggtctat cccctgcacc ttgacgcgtt ccaccttggc atagacccgc
      901 tcaccagtca agctgccctg gcccagaaac cactggaaca tctcgttggg gacgcgttca
      961 acacggggtt ggcctgaagc gctctctcca tatataaaaa gatctggatg tcgtgtattt
     1021 tcatcggcgg tttttatgcg cacaaacagg cgtaccgggt ggtcaaattg acgctggata
     1081 agcacctcgc caggaccggt tatttcaccc agtttttccc cgcgggtact atagtggctg
     1141 tctctatccc aattcaggta ggagtgacgc ctgggtgccc aagctgcatc caaagccgta
     1201 tgcaggtgat cggcataaga ctggagcacg ggtaaagact caacccgttc gagaatttga
     1261 cccatctgga tcaaggcctt acattcgcga tgcaatagcg cacacagcgc tggactctcc
     1321 gcagatgaga tatcgacgcc ttgcgaccat tcatgccacc gtgagtacac agggtgatct
     1381 tcgaagcctg cctgcatggg atggtcccac tcaggaacac catccccatc ccggtcatga
     1441 cgatcatcga accaacgata gataaactta agcagcggtt cgaaaacatc ttctaaaaac
     1501 acacgatcct ggctaatctg gaaaatttcc cagctcaatg tggccaggat aggcatggcc
     1561 agcaggccgc tgcgttgtcc cgcaagacct ggtttccaat ctagaccgcc gtcctcttcc
     1621 cggacagcga tgtaatttcg taccagccct tgcagcaaag acgtcccacc tgggagcaat
     1681 aatcccgcca ggtaataaat ctccagcggt ggctggccgt tccacaggtg attgtagtcg
     1741 cccccgtcac cacgtagaga atatccttga tcgggctgtc gtgataaaac aattgaaggg
     1801 aagggcagat gctgtgtagg accaacaaaa gtcgccgcag ccaatttctg agaaaaggca
     1861 aaaaccgcat cccaatcggg atcgccagta tagatctcta attgagcgga attgaccaat
     1921 tcgacgcgtg caatttctgc ctcccagggg caggcaacca tctggcgggc gagtgaaaaa
     1981 gaggtttcga catcttgcaa ggcagcatgg caccaggtga atcgtcgcga ggctctgggt
     2041 ggcaattcaa tctccaggtt aagcgctggg taagggctcc ccaccgcctg gggtccgcca
     2101 gtgacgaaca ttaccggcgt cagcccgcct gtcatcccag ccaacgcggg agcattttgc
     2161 atctcgatcg gagccatgcg ctgcccttcc gttgggttca gttgggcaac taggctaagg
     2221 cgtatcaggc gtgccttgga gctgttgttc atcagtccaa ccctgcccgc caccgcatgg
     2281 gattgaggaa cccaatactc cagctctacg tctatatcgg aaagaggaga gcattgcacg
     2341 ctcagataat ttgggaagaa attggtcacg actggagggg cagcaaactc tgctggagcg
     2401 gttaccatcg tatctccctc gccaaaacgc gggaacacac gcaccgagcg ggcacgcagc
     2461 ccgaaagtgg tttgcagtgc cagagcaggc gggtcacctc cccctatcct gagttcccaa
     2521 atttgatcgt cgcaataatc tgtaggcccc aggcgagcgt cagccatcaa ggtcaacgcc
     2581 agcggatcgc cagctttcag gttccatttt cgcatggtac attgtattgt atggtatgtt
     2641 gccctaaagg tcaaacggat cagggcaatc gcatttgggc gatttcccac cactgaggag
//
LOCUS       DBNHOH010000525         1663 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_864919, whole genome shotgun sequence.
ACCESSION   DBNHOH010000525 DBNHOH010000000
VERSION     DBNHOH010000525.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_864919"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1083)
                     /locus_tag="AC16V7_09185"
     CDS             complement(154..1083)
                     /locus_tag="AC16V7_09185"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6257334.1"
                     /translation="MKQRLPYPELAVVIASAAWGLFWIPLRAFERRGLEPAWATLTQF
                     LAPLAILLPVAVIRWQRRQPTGIHQYRTGLLIGAAVALYLESLLLTDVARALILFYAM
                     PAWGTVLEVGLMGRPLTRWRGIALLLSLGGMLTILGVGDSLSLSMNLGDLMALLSGIL
                     FAFGAMRVREAPETSVFEQILAFFLYGSVAALALSLLPLAQLGQPPSVDQLITLAPWL
                     ALIAVGFLIPVMWGVYWGSRHVDPGRLGILLQIEAIVGIGSAALLAGEPFGVRQALGA
                     VLVISAGLVEVIGNQRRKTANAELPTSRRLSGE"
     gene            1467..>1663
                     /locus_tag="AC16V7_09190"
     CDS             1467..>1663
                     /locus_tag="AC16V7_09190"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR6257335.1"
                     /translation="MSEENKAIIKRFYEELDKGNFDIYDELCTPDFISHFPGNPQPID
                     RETRKQTSRMFYAAIPDCKHTL"
BASE COUNT          449 a          449 c          433 g          332 t
ORIGIN      
        1 tggctgaggt aaagaaggtg ctccccctat agattaactg ggtgagggca acggtggtgc
       61 cagctactta cgtcatgtgg tttcccgtca tggatggaaa gacggtaagc cgcccatcgg
      121 actgcgtgga gccgacctgc tccgctggcg gcatcactca cctgagagcc ggcgcgaagt
      181 tggcagttcc gcgttcgctg ttttacgtct ttggttcccg atgacctcca ccagaccggc
      241 gctgatcacc aggacggcac ccagggcctg ccgtacccca aacggctctc ctgcaagcaa
      301 ggccgcgctg ccaattccca cgatagcctc gatctggagc aggatgccca gtcgaccagg
      361 atcgacgtgg cgggaacccc agtagactcc ccacattacc gggatcagga atccaaccgc
      421 gatcagggcg agccaaggcg cgagtgtgat caattgatcg acggagggtg gctgcccgag
      481 ttgggccagg gggagcaggg agagagccag tgcagccacc gagccataaa ggaagaatgc
      541 aagaatctgc tcaaagactg aggtctcagg agcctcgcgc acccgcatag caccgaaggc
      601 aaacaggatt cccgatagca gggccattag gtctcccagg ttcatggata acgagagtga
      661 atcacctacg ccgagaatag tgagcatccc tcccaagctg agcagaaggg caataccgcg
      721 ccagcgggtg agcggacgcc ccatcaggcc cacttctaaa acagttcccc aggctggcat
      781 ggcgtagaag agaatcaatg cacgtgctac gtcagtgagg agcaggctct caagatacaa
      841 cgcaaccgca gcaccgatca gcagtccagt acgatattgg tggatacctg tgggctgtct
      901 acgctgccac cttatgaccg cgaccggcag aaggatggca aggggcgcga gaaactgggt
      961 cagggtagcc caggcaggct cgaggccgcg gcgttcaaac gctctcagag gaatccagaa
     1021 caatccccat gcagctgatg caattacgac ggcaagttca gggtagggaa gtctttgttt
     1081 catttagact gtaggaaatt tctttacatt ggccaggaat agagaaatag taagtaaaag
     1141 tatacactaa caaattcaga caaaagacaa aagaagtgat agccgatttt gtgggctttc
     1201 gatacttgct gcacaaaata atgattatgt ttaccttaat aatcgcattc gattataatc
     1261 aatatagata acaccacaag ctacacgaac cacgttttaa tctgtggtcg tttaccccct
     1321 gggttaatct tggtgatgag gcagcataga atatatatcc cacatcccgc acctggttgt
     1381 gcaacattaa tctaccctgt caaatctcag cacatataat caccaacact acccaaattt
     1441 agctcaagcc agaaaggagg cttttgatgt cagaggaaaa caaggccatc atcaagcgct
     1501 tctatgaaga gctcgataaa ggcaattttg atatttacga cgagttgtgt acccctgact
     1561 tcatctcgca ctttcccggc aacccccagc ccatagaccg ggagacccgc aagcaaacct
     1621 cccgcatgtt ctatgcggct atacccgact gtaagcatac cct
//
LOCUS       DBNHOH010000526         4241 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1569786, whole genome shotgun sequence.
ACCESSION   DBNHOH010000526 DBNHOH010000000
VERSION     DBNHOH010000526.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4241)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4241
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1569786"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /gene="ruvB"
                     /locus_tag="AC16V7_09195"
     CDS             complement(<1..1023)
                     /gene="ruvB"
                     /locus_tag="AC16V7_09195"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR6257336.1"
                     /translation="MPEMPSRLVNPLPQPEDRLDAALRPKLLADLIGQERVKENLAIL
                     IEAARQRAEPLDHVLFYGPPGLGKTTLAHILANEMNVNIKITSGPAIERPGDLAAILT
                     NLRAGDVLFIDEVHRLGKAIEEVLYPAMEDFALDIVIGKGPSARSIRLKLPRFTVIGA
                     TTRLALVTAPLRARFGAVYRLNFYELPAMQAIVDRAAGVLGVEADPEGVDEIARRGRG
                     TPRVALRLLRRVRDFAEVRANGVITRHVARGALELMEVDSLGLDDIDRRVLLTIIEKY
                     RGGPVGLTTIAASISEEPDTIMDVVEPYLLQLGFLDRTPQGRSATRLAYEHLGLSVPD
                     DLQPRLL"
     gene            1193..1819
                     /gene="upp"
                     /locus_tag="AC16V7_09200"
     CDS             1193..1819
                     /gene="upp"
                     /locus_tag="AC16V7_09200"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HIR6257337.1"
                     /translation="MPNLFPSPHPLVAHKVTRLRDKNTEPKIFRELIREIATLLTYEA
                     TADLELKPKSVETPLAPYSGTEVQETIGLVPILRAGLGMVEGVWNLMPAAEVWHIGLY
                     RDERTLKPVEYYNKLPIEPTVAMCLILDPMLATGGSAVATVDVLKRWGVRKIKYVGII
                     GAPEGIRLMSEHHPEVPIHLAAVDERLNEHGYILPGLGDAGDRQFGTG"
     gene            complement(1835..2455)
                     /locus_tag="AC16V7_09205"
     CDS             complement(1835..2455)
                     /locus_tag="AC16V7_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015078190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HIR6257338.1"
                     /translation="MKQKHFIDSHKAVTFIYILTMIVLYKQWENPTAWVYLALHGTYG
                     ILWVLKSRIFPDWQWEQPTGWLYGLVIWGGLSLYWVAPWLLMARGVQAPAWYLGLCIS
                     LNIFGVFFHFVADMQKYTTLSLQPNRLFTGGVFSKIRSINYFGEMLIYFGFSLLAMHW
                     LPVVILLVWIIFVWLPFMRKKEHRLSRYPEFAAYKNRTKMFIPFLF"
     gene            2687..>4241
                     /locus_tag="AC16V7_09210"
     CDS             2687..>4241
                     /locus_tag="AC16V7_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR6257339.1"
                     /translation="MSTDKIGRYIIKAELGRGGMSTVFHAYDPSFERDVAIKVLPAAF
                     LHDPQFRARFDREAKMIANLEHPAIVPVYDFGEQDGLPYIVMRYMAGGSLADRLAKGA
                     LSFEETSRIISRLAPALDAAHARGIIHRDLKPGNILFDQYSNAFLSDFGIARLTESSA
                     TLITGESIVGTPAYMSPEQVQSNKTIDRHSDIYAMGVLTFQMLTGQAPYQAESPAKVM
                     MMHLLEPVPTILDKKNDLPQGTEEIISRSMAKEPADRYSTTAELASALEALVHGSQAE
                     IQIQPVAPRGLSTSGSVRGAAKPIKQPSPVVLPDESLATTPGLPGVQSVATTQPTRRT
                     LTLPLVLIIILLVGGIGAMSVFLFSGRQGSSFPLLGAVQASATSASTNTPVPPTQTTA
                     PQPTSTIPVLMLDTPSAEPSPTPMATPSPTITATSQPTTLVLGGADKLAFLSGSNVWA
                     ANLDGSELVQLTDDGANKTSLQWTPDGQALIYVSGKCVQMVSLDGATIENLTCINYID
                     SLKSFDISPD"
BASE COUNT         1103 a         1186 c         1039 g          913 t
ORIGIN      
        1 caacaatctg ggctgcaggt catctggcac cgataacccc aggtgctcat aggccaaccg
       61 ggtggctgaa cgaccttgtg gtgtgcggtc taagaaaccc agttgcagca ggtagggctc
      121 gactacatcc ataatggtat caggctcttc gctgatggag gcggcaattg tggttaaacc
      181 aaccgggcca ccgcgatatt tttcaataat ggtaagcaag acacgacggt cgatgtcatc
      241 taaaccgaga gaatcaacct ccatcagttc gagtgcaccc cgggcaacgt gacgagtgat
      301 taccccattt gcgcgcacct cggcaaaatc acgcacacgg cggagcaaac gcagtgcgac
      361 ccggggtgtg ccccggccgc gacgggcaat ctcatccact ccttcggggt cagcttccac
      421 gcccaacaca ccagccgccc gatccacaat ggcttgcatt gcaggaagtt cgtaaaaatt
      481 caggcgatac accgcaccga agcgcgccct caggggagct gtaaccaagg ccagtcgtgt
      541 tgttgctccg atgactgtga agcgcggcaa ctttagacgg atggaacgcg ccgagggacc
      601 cttgccaatg acgatatcca gggcaaagtc ttccattgcc gggtacagca cttcttcaat
      661 ggctttcccc aagcggtgaa cttcgtcaat gaagagcaca tccccggccc gcaggttagt
      721 gagaatggca gccaggtcac caggtcgctc gatggcagga ccagaggtga tcttgatatt
      781 gacattcatc tcgtttgcca ggatgtgcgc cagtgtggtt ttccccagtc cgggcggccc
      841 ataaaacagg acgtgatcca atggctcagc gcgctggcga gctgcttcga tcaagatggc
      901 tagattttcc ttgacacgct cttgcccgat tagatcagcc aataactttg ggcgcaatgc
      961 tgcgtccagg cggtcttcag gttgaggtaa agggttaacc agacgcgagg gcatttcagg
     1021 catgcagcca ttataacgcg ttagcgcccc ggatgccaaa gccatgaagc gaagatgcac
     1081 caactcaata caaataacct atgatggatg tttcatttgc agcccataaa ggatataatt
     1141 tcctgagtac gacctttcaa tcatttcgaa ccctccgaac gggagtcttc tcatgccaaa
     1201 tctattcccc tctccacacc cactggttgc ccataaggtt acccgcttac gagataaaaa
     1261 cacagaacct aaaatatttc gtgagctaat tcgtgagatc gccaccctgc tgacctacga
     1321 agccaccgct gatctggaac taaaacccaa gtcggtggaa acaccgcttg ctccttattc
     1381 gggaacagag gtacaagaaa cgattggcct ggtgccgatt ctgcgggcgg ggcttggcat
     1441 ggtcgaaggg gtttggaacc tgatgccagc agctgaggtc tggcacatcg gcctctatcg
     1501 tgacgaacgc acactcaagc ctgtagagta ctacaacaaa ctaccgatag agccaaccgt
     1561 agcgatgtgt ttaattctgg accccatgct ggcaacaggt ggttcggctg tcgctacggt
     1621 ggatgtgctc aagcgatggg gggttcgcaa gatcaagtat gtcggcatca tcggtgctcc
     1681 agaaggcatc cgactgatga gcgaacacca cccagaagtt cccatccacc tggcagcagt
     1741 cgatgagcgc ctaaatgagc atggctatat tctgcccggt ttgggcgatg ccggcgacag
     1801 gcaattcgga acgggctaag aaacgctcag cttttcagaa caagaagggg ataaacatct
     1861 tggtgcgatt tttatacgct gcaaattcgg ggtagcgcga aagacggtgt tcttttttgc
     1921 gcataaatgg caaccagacg aagatgatcc aaaccaacaa gatcactacc ggtaaccagt
     1981 gcatcgccag caagctaaac ccgaaataga tcaacatctc gccaaaataa ttgatactgc
     2041 gtatttttga aaacacacct ccggtgaaca ggcgattggg ctgcaagctc aaagtggtat
     2101 atttttgcat atcagcgaca aagtgaaaga acacgccaaa gatattcagg ctgatgcaaa
     2161 gacccaggta ccatgctgga gcctgcacac cgcgagccat cagcagccag ggtgccaccc
     2221 agtacaagct cagccctccc cagatcacaa gaccatacag ccagccggta ggttgctccc
     2281 attgccaatc ggggaagatt cggcttttca atacccacag tatgccgtat gtcccgtgca
     2341 aggcaaggta aacccacgca gtgggattct cccattgctt gtatagtacg atcatggtta
     2401 atatatagat gaatgtaact gctttgtgcg aatcgataaa atgtttttgc ttcaaaatag
     2461 caccttttct gtcaattatg caaggtcgag tggcgtaact tgcacaacca ctaccattga
     2521 cgtacaattc gaaaatagaa cacatccact aggttgtgaa ccaacgcgac gttagcatta
     2581 tatccaaata aaggcttctc ttccccaaaa ttaaaaagca gtctcccagg tttagtaaaa
     2641 ctgaggcttc gcatctaata gcggaaatct cacaaaagca aaatcaatgt caaccgacaa
     2701 aatcggacga tacatcatca aagcagagct ggggcgtggc gggatgtcaa ccgtatttca
     2761 cgcctacgac ccgagcttcg aacgcgacgt agccattaaa gtcttacctg cggcttttct
     2821 tcacgatccg cagtttcgag cccgcttcga ccgcgaggcg aagatgattg ctaacctcga
     2881 gcacccggcg attgtgcccg tttacgattt tggtgagcaa gatggcctac cttacatcgt
     2941 catgcgctac atggctggcg gatcactggc agacaggtta gccaaagggg ctctgtcgtt
     3001 tgaggagacc agccggatca tatcccgctt agcccctgca ctggatgcag cacatgcgcg
     3061 cggcatcatt caccgcgatc tcaagccagg caatatcctc ttcgaccagt acagcaatgc
     3121 atttctttcg gatttcggca ttgcccgctt aacggaatcc tcagccacac tgatcactgg
     3181 tgagagcatt gttggcacgc cggcttacat gagcccagag caagtgcaga gcaataaaac
     3241 tatcgacagg catagcgaca tctatgcgat gggtgtgctc accttccaga tgctcaccgg
     3301 gcaagctcca taccaggcgg agtcccctgc aaaagtaatg atgatgcacc ttttggagcc
     3361 agtccccacc atcctggaca aaaaaaacga cctgccacag ggaactgagg agatcatctc
     3421 ccgttctatg gcaaaagaac cggcagaccg ttattctaca acagctgagc tggcttctgc
     3481 cctggaggca ttggtacatg gctcgcaggc agagatccaa atccagcctg tggctccacg
     3541 cggcttgtcc acttctggga gtgtgagggg ggcagcaaaa ccaatcaaac aaccatcccc
     3601 tgtggttctg ccagacgaat ccctggcaac tacaccagga ttacccggcg ttcaatcggt
     3661 agccacaacg caaccaaccc gtagaactct tactctaccg ctagttctca ttatcatcct
     3721 gctcgtgggt ggtattggcg cgatgagcgt tttcttattc tctgggcgcc agggtagttc
     3781 atttcctcta ttgggtgcag tacaggctag cgcaacctct gcatccacaa atacgcccgt
     3841 ccctcccacc caaaccactg cgcctcaacc caccagcacc attccagtac tgatgctgga
     3901 tacgccctca gcagagccat cgcccacccc aatggcaacg ccttccccca ccatcacagc
     3961 cacatctcaa cctaccacac tggttcttgg cggagccgat aagctggctt tccttagtgg
     4021 atccaacgta tgggcggcta acctggacgg aagcgagcta gtgcaactga ccgatgacgg
     4081 agcaaacaaa accagtttgc agtggacgcc agatggccag gccctaatct atgtttccgg
     4141 taaatgcgtg caaatggtct cgctggatgg agcgacaatt gaaaacttaa cctgcatcaa
     4201 ttacatcgat tccctaaaat ccttcgacat ctccccggat g
//
LOCUS       DBNHOH010000527         2148 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_415512, whole genome shotgun sequence.
ACCESSION   DBNHOH010000527 DBNHOH010000000
VERSION     DBNHOH010000527.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_415512"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..601)
                     /locus_tag="AC16V7_09215"
     CDS             complement(101..601)
                     /locus_tag="AC16V7_09215"
                     /inference="COORDINATES: protein motif:HMM:NF044704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009303 - rRNA transcription [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HIR6257340.1"
                     /translation="MEFQVGDKVVHWSYGPGEIIQLDEKSISGQNALYYVVQIRDLTL
                     WVPVEGGDQSSLRLPTSTREFRRLFKILRNPGEKISDDRYERKTQLTERMREGTLESI
                     CGIIRDLTTYSYSKKLNDYDASLLERAKKFLLEEWKISLSVPLSDAQRELDILLEEGR
                     IVAADE"
     gene            complement(705..1070)
                     /locus_tag="AC16V7_09220"
     CDS             complement(705..1070)
                     /locus_tag="AC16V7_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA recognition motif domain-containing protein"
                     /protein_id="HIR6257341.1"
                     /translation="MKLYVGNLSYNSTEEDIRTLFAQAGTVVSVDLIKDRFTGSSKGF
                     AFVEMSSQSEGENAIRMFDGYSLDDREIKVNVARPREERSSGGFDNRRGGYGGGRGQG
                     GQGGRGGRDRRGGGGTRRY"
     gene            complement(1441..>2148)
                     /locus_tag="AC16V7_09225"
     CDS             complement(1441..>2148)
                     /locus_tag="AC16V7_09225"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6257342.1"
                     /translation="AWPARSELGYFALLGFLGIAFHQWLQSTGLLTAQATTTAWIIAS
                     TPIFMALLGWSFLKENLGRLQVAGIALAAVGVLLVVSKGDFSSLAGGKFGTPGDFLIL
                     ISAPNWAVFSALSQRGLQRHPATRMMLYVMSFGWLFSTLLLFTGPGVLELGQLTRDGW
                     LAMIFLGVFVSGLAYIAWYDALQAIPSSRVGAFLYLEPLVAVGVAAVVLSEPLLLSSL
                     LGGGTILFGVWMVNRQG"
BASE COUNT          566 a          620 c          500 g          462 t
ORIGIN      
        1 gcgataccac cagcccaagc gtgaggcgat ttccaattgg gtgattctga tctgtcttta
       61 ttgttttgct gatacaatca agtgatgcgc tcgcagcact tcattcatcc gcagcgacga
      121 ttcgcccctc ttctaaaagt atatccaatt cgcgttgagc atcacttaat gggacagaga
      181 gggaaatctt ccactcctcc agcaagaatt ttttagcacg ctccagcaag gatgcatcat
      241 agtcatttag tttctttgaa tagctatagg tagtgagatc acgaataatg ccgcagatcg
      301 actcaagcgt cccttctcgc atgcgctcag tcaattgagt ttttcgctca tagcgatcgt
      361 cagaaatttt ctccccagga ttgcgtaata ttttaaaaag tctcctaaac tcgcgcgtcg
      421 aggttggcaa tcgaaggcta gattgatcgc ctccctccac tggaacccat agggtgaggt
      481 ccctgatctg gaccacataa tacaaagcat tctggcccga gatgctcttc tcgtccagct
      541 gaatgatctc tcccggacca tacgaccagt gaactacttt atcacctact tgaaattcca
      601 caattcctac tttaagaagc taatcagttg gcgagaaagt aaggggttgg cggacaatgc
      661 cgcccaaccc ccaatttttt tctttaacga aacaagttct gattttaata cctgcgggta
      721 ccaccgccac cgcgtcggtc ccgaccgcca cgaccacctt gaccgccttg accacgaccg
      781 ccgccgtagc cgccgcgccg attgtcaaag ccaccagaac tgcgctcctc gcgtgggcga
      841 gccacattta ccttgatctc gcggtcatcc aagctatatc catcaaacat acggatggcg
      901 ttctcgcctt cactttggga gctcatctcc acaaatgcga agcctttcga agagcccgta
      961 aagcgatcct tgatcagatc cacggagacc actgttcccg cctgggcgaa caatgtacgg
     1021 atgtcctctt cagttgagtt ataggacaga tttccaacat agagcttcat tttcaaatga
     1081 tcctttcggg ctattgagta gataggcaat aaaattccgc catccttaca aacaggaatg
     1141 gcggttatcc aacacgcaaa tcctttgaca gggacaacta cattttacca tattctgacc
     1201 ccagccggta aaaagcccct ttgaaatcag ggcaattgca taggccctga ggggcaggcg
     1261 caatcccgca aaacgagggt tttgggtgtt ttggtggggg gataccccac caaaacaccc
     1321 aaatttctct cttcagcggt gggaaatcac cctaatgcga ttgccctacc tttgaaatca
     1381 gggcatttac aacacccttc tgggcatgtg caacctcgcg aaacgggggt tggggtgtca
     1441 tcaaccctgg cgattcacca tccacacccc aaacaagatc gtccctcctc ccaagagcga
     1501 agacagcaac agcggctcac tcagcacgac tgccgccacg ccaaccgcca cgagtggctc
     1561 gaggtaaaga aatgcgccga cccgcgaaga ggggatagcc tgcaaagcgt cataccaggc
     1621 gatataggcc aaccccgaca cgaacacgcc taggaagatc atcgccagcc agccatctcg
     1681 ggtcaattgc ccaagctcaa ggacgcccgg gccagtaaac aataacaagg tagagaataa
     1741 ccagccaaag gacatcacgt acaacatcat gcgcgtggct ggatgccgct gcaatccccg
     1801 ctgcgaaagg gcagagaaca ccgcccagtt gggtgcgctg ataaggatga ggaaatctcc
     1861 cggcgtacca aatttccctc cggccaggct agagaaatca cctttgctta ctaccagcaa
     1921 cacacccacc gcagccagag cgattccagc aacctgtagc ctgccaaggt tttccttgag
     1981 aaaggaccag cctaagagcg ccataaatat cggcgtggag gcaatgatcc aggcggtggt
     2041 ggtcgcctgg gcagttaaca gtccagtgga ttgcagccat tgatggaaag cgatgcccaa
     2101 aaagcccaac aaggcgaagt agcccagctc gctgcgggca ggccaggc
//
LOCUS       DBNHOH010000528         2227 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_864950, whole genome shotgun sequence.
ACCESSION   DBNHOH010000528 DBNHOH010000000
VERSION     DBNHOH010000528.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_864950"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(308..1981)
                     /gene="ettA"
                     /locus_tag="AC16V7_09230"
     CDS             complement(308..1981)
                     /gene="ettA"
                     /locus_tag="AC16V7_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HIR6257343.1"
                     /translation="MTDQVIYSMARVGRIYPPNRQVLRDISLGFYYGAKIGVLGLNGA
                     GKSTLLRIMAGVDRDYVGEVFLTKGYTVGLLEQEPALDPQKTVRQVVEEAVQPIMDSL
                     AQYEAINTRFAEPDADFDALIVEQARLQEELEKHDAWTLDSRLEQAMDALRCPPGDAP
                     IAVISGGERRRVALTRLLLTEPDILLLDEPTNHLDAESVAWLEHHLQQYKGTVIAVTH
                     DRYFLDNVAGWILELDRGFGIPWRGNYSSWLEQKQDRLRREEKQESKRLRTLERELDW
                     IRMSPKARQSKGKARYTAYERLLSQEYEKRREDQEIYIPPGPRLGDIVIQFEGVSKGY
                     GERLLLDDLTLSIPPGSIVGVIGPNGAGKTTLLRLVTDQEKPDEGTVRLGETVNLAYV
                     DQSRDTLDGDKTVWEEISGGEEQLDLGGRKRNSRAYVASFNFLGSDQQKLVKNLSGGE
                     RNRVHLAKVLKEGANLILLDEPTNDLDVNTLRALEDALESFNGCAVIVSHDRWFLDRV
                     CTHILAFEGESQVRWFEGNWSDYEAERKKRLGIDADQPHRIKYRTLKRS"
BASE COUNT          480 a          675 c          575 g          497 t
ORIGIN      
        1 aaaataatag accagcgatc tggccgaccg caaccatccc caatggcata tcattgctaa
       61 tgactcaccc ccacaatact tcgcacttag gagtctttgc gcgaactttc agattaataa
      121 gggcaaattt atctaaacca acaaaaacga aaagtacaca ggacgaaaat taaggtatta
      181 ttgtcttgct cgccgttatc actaccacac accatcatcc tttttcggcc attaccgttg
      241 cacacaggcg agcaatatcc tagaccatgc ccgtcgaccg ccgtacgggc gtggtttttt
      301 tatcccatta tgatcttttc aacgtgcggt acttgatgcg gtgcggctgg tcagcgtcga
      361 ttcccaggcg tttcttgcgc tcggcttcgt aatccgacca gttgccctca aaccagcgca
      421 cctgcgattc gccttcgaag gccagtatat gcgtgcagac gcggtccagg aaccagcggt
      481 cgtgactgac gatcaccgca cagccgttaa agctctcgag agcatcctct agggcgcgca
      541 gggtgttcac gtctaggtca ttggtcggct cgtcgagcag aatgaggttc gcgccttctt
      601 tgagcacctt agccagatgg actcgatttc gttcgcctcc agaaaggttc ttcaccagct
      661 tctgctggtc ggagccaaga aagttgaagc tggcgacata agcacgggag ttgcgctttc
      721 gccctcccag gtctagctgc tcttccccgc cggaaatctc ctcccaaacc gtcttgtcac
      781 cgtccagggt atcccggctc tggtccacgt aagcaagatt gaccgtctcg cccaggcgga
      841 ctgtgccctc atccggcttt tcctggtctg tgaccagacg caacaaggta gtcttccctg
      901 ctccattagg cccgatgacg cccacgatgc tgcccggcgg tatgctcaac gtcagatcat
      961 caagcagcag ccgctcgccg taacctttgg agacaccttc gaactggata acgatatcgc
     1021 ccaggcgcgg gccgggcggg atatagatct cctggtcttc gcggcgcttt tcatactcct
     1081 ggctgagcaa gcgctcgtag gcggtgtagc gagccttgcc tttggattgg cgcgccttgg
     1141 gtgacatgcg aatccaatcc agctcgcgct ccagggtgcg gaggcgcttg ctctcctgct
     1201 tctcttcccg gcgcaggcga tcctgttttt gttccagcca ggaagagtag ttgcctcgcc
     1261 aggggatgcc gaaaccgcga tccagctcca atatccagcc agccacatta tccaggaaat
     1321 agcggtcgtg ggtcacggcg atgaccgtgc ccttgtactg ctgcaagtgg tgttccagcc
     1381 aggctaccga ttctgcgtcc aggtggttag tgggttcatc cagcaggagg atatccggct
     1441 cagtgagcaa caagcgggtg agggccaccc gccgcctctc gccgcccgag atcacggcaa
     1501 tgggtgcgtc tcctggaggg cagcgcaagg cgtccatagc ttgctccagc cggctgtcca
     1561 gcgtccaggc gtcgtgcttc tccaactcct cttgcaagcg ggcctgctcg acgatcagtg
     1621 cgtcgaaatc ggcatctggc tcggcaaaac gggtattaat cgcctcgtac tgggccaggg
     1681 agtccatgat cggctgaacc gcctcctcaa cgacctgccg gacggttttc tgcgggtcga
     1741 gagcaggttc ctgctccagc aaccccacag tgtaaccttt agtgagaaag acctctccca
     1801 cataatcccg gtccacccca gccatgatac gcagcaaggt gctcttaccg gcaccgttca
     1861 gccccaacac cccgatcttg gcaccataat aaaagcccag ggagatatcc cgcaggactt
     1921 gtctgttggg cgggtagatg cgccctacac gcgccatgga atagatgact tgatctgtca
     1981 taaagtgatc tcctgcgcac taccgtgcgc aattacgaac ctgaagaaac gtttgttgtg
     2041 cgccacaact ctcgtgcgca attacgaacc tagagaaatg ttggttgtgc accacaaccc
     2101 tcgtgcgaaa ttacgaacct agagaaacgt tggttgttct ccacaactct cgtgcgcaat
     2161 tacgaaccta gagaaacgtt ggttgtgcac cacaactctc gtgcgcaatt acgaacctag
     2221 agaaacg
//
LOCUS       DBNHOH010000529         1912 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1185377, whole genome shotgun sequence.
ACCESSION   DBNHOH010000529 DBNHOH010000000
VERSION     DBNHOH010000529.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1185377"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="AC16V7_09235"
     CDS             <1..712
                     /locus_tag="AC16V7_09235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR6257344.1"
                     /translation="ATAQEEPIIESLEPMPEEAESLEARPDLPSWLTDVEMTKTPEAE
                     AGWAPPVEKQPESVIEEETPVFAEQSAETLPETVNLNEAGQAELEQLPGVGFTRAQAI
                     INYRQEHGPFSNVEELINVYGFGEVLVNSLIDKIKVVEAQPAEVLPEAVGDNQVILIQ
                     ARNALIQGNISEALVHYTHLVKEQQFLPEVIQDLNEALYRFPVDVSIWEALGEAHLRA
                     GRLQEALDALTKAEELIR"
     gene            782..1231
                     /gene="ndk"
                     /locus_tag="AC16V7_09240"
     CDS             782..1231
                     /gene="ndk"
                     /locus_tag="AC16V7_09240"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HIR6257345.1"
                     /translation="MEKTLVLVKPDGVQRGLIGEVFSRLERRGLRLVGAKFMAVSQEL
                     AETHYAIHKGKPFYEGLIRYITSAPVMAMVWEGPNAIAAVRQTMGSTRPTEAAPGSLR
                     HDFALEVGRNLTHASDTPENGEIEVALWFNQDELVAWERATDRWIFE"
     gene            1258..>1912
                     /locus_tag="AC16V7_09245"
     CDS             1258..>1912
                     /locus_tag="AC16V7_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR6257346.1"
                     /translation="MHTPQQIQSQLEAILPNVQKPGRYTGGELNQVLKEWDSVQTRLA
                     FVFPDIYDIGMSNLGLAILYDLINQRSDALAERAYNPWSDMESALRHAAIPLYSLESK
                     HSLADFDIVAFSLPYETLYTNALNALDLAGIPLFSTERDETHPLVIAGGHACYNPEPM
                     HAFIDAFVVGEGEDVIQEIIDVHQLWRQSGRGRNELRISLSTIWGVYVPSLYQAEYHR
                     "
BASE COUNT          498 a          473 c          487 g          454 t
ORIGIN      
        1 agcaactgct caagaggagc caatcatcga gtcacttgag cctatgccag aggaagccga
       61 atcgctcgaa gcccgacctg atctccccag ctggttgacc gacgttgaga tgaccaagac
      121 acccgaggcg gaggcaggct gggcaccccc tgtagaaaaa caaccggaat cagtaatcga
      181 ggaagaaacc cctgtttttg cggaacagtc tgctgaaaca ttaccagaga cggtaaatct
      241 caatgaagcc ggacaagcag agctcgagca gttgcccggc gtgggattca cccgagcaca
      301 ggcgattatc aactaccgcc aagagcatgg cccgttcagc aatgtagaag agctgatcaa
      361 tgtctacgga tttggtgaag ttctggtcaa ttcgcttata gacaaaatta aggttgtcga
      421 agcccagccg gctgaagttt tgcctgaagc agttggtgac aaccaggtaa tcttaattca
      481 ggcacgcaat gcccttattc aaggtaatat ctcggaggct ttggtacact atactcacct
      541 tgtcaaggag caacaattct tgcctgaagt catccaggat ctgaatgaag cactttaccg
      601 gttcccagtc gatgtctcca tttgggaagc ccttggggaa gctcatctgc gcgctggacg
      661 gcttcaagaa gcactggacg cactcacaaa agctgaggaa ttgattagat agacattgaa
      721 ggtccaatgc agtttgaaga aaggcatccc aagctgcaat atttttaatt tggagaatta
      781 cttggaaaaa acacttgtcc ttgttaaacc tgatggtgtg cagcgtggat tgatcggcga
      841 ggtgttttca cgtcttgaac gacgaggcct tcgcctggtg ggagcgaaat ttatggcagt
      901 tagccaggag ttagccgaga cacactacgc aatccataaa ggtaaacctt tttatgaagg
      961 tctaatacgc tacatcacct ccgcgccagt gatggcaatg gtttgggaag gtcccaacgc
     1021 catcgccgct gtgcgccaga cgatgggttc cacgcgtccc accgaggccg cacctggttc
     1081 actacgacac gatttcgccc tggaagttgg gcgcaattta actcacgcat cagatacacc
     1141 cgagaatggt gaaatagaag ttgccctgtg gttcaaccaa gacgagctcg ttgcctggga
     1201 gcgcgctaca gatcgctgga tattcgagta agtcttgtgt ttttaggata attattggtg
     1261 catactccac aacagataca atctcaactt gaagctattc ttccaaacgt tcagaaacct
     1321 ggacggtata ccgggggcga gttaaatcag gttctaaaag aatgggatag cgtgcaaacg
     1381 cgcctggctt ttgtatttcc tgatatctac gatatcggca tgtcaaattt gggattagcc
     1441 attctatacg atctcatcaa tcagcgcagt gatgcgctgg ccgagcgcgc ttataacccc
     1501 tggagcgaca tggaatccgc cttgcggcat gctgccatac ctctttattc gttggagagc
     1561 aagcattcct tggcagattt tgatattgtt gccttctcgc ttccctacga aacactgtat
     1621 accaacgcgc taaacgccct agatctggca ggaatccctc tattctccac cgaacgggat
     1681 gaaacgcacc cccttgtgat tgccggaggc catgcttgtt ataaccctga gcccatgcac
     1741 gcattcattg atgcttttgt ggtcggggaa ggcgaagatg tgatccagga gattatcgat
     1801 gtgcaccagc tttggcgcca gagcgggcgc gggcggaatg aactgcgtat atctctttca
     1861 acaatatggg gggtgtacgt cccatcacta taccaggcag aataccatcg cg
//
LOCUS       DBNHOH010000530         5015 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1120918, whole genome shotgun sequence.
ACCESSION   DBNHOH010000530 DBNHOH010000000
VERSION     DBNHOH010000530.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5015)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1120918"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="AC16V7_09250"
     CDS             <1..477
                     /locus_tag="AC16V7_09250"
                     /inference="COORDINATES: protein motif:HMM:NF012263.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008121 - quinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HIR6257347.1"
                     /translation="PDHAYDSVQYITTQVPMGWLIRGLHHWGASAMVVVVVLHMLRII
                     FYGAYKFPREVTWFTGVILLIVTIGFGFTGYLLPWDQKAYWATTVGTRIAGTPPVIGE
                     WILLIIRGGEELSAVTLARFYGVHIWVLPTVLLLITLIHLYLVIRIGISAVPGREE"
     gene            481..2634
                     /locus_tag="AC16V7_09255"
     CDS             481..2634
                     /locus_tag="AC16V7_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012262.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6257348.1"
                     /translation="MEEKEKQQYYEKYRKAKEKGIFFYPDAIFKDAVVVFVIFLILIG
                     LAYFIGAPLEERANPADTSYTPRPEWYFLFLFQLLKYFPGELEVLGVVVLPTLAIILL
                     FALPFLDRSSRRHFRSRPLFVGVTALMMIGVVALTVQSYREAPPPAEEIAGDQTAALY
                     AKNCAGCHGPSIDVAAGTNLHAIIAQGKHEGMPAWSADLTSDQIDALAGFVLSPGGSK
                     LFTANCGECHSAADMVAGDPIELKKAIEQGKEYPVHADVDIPPWNEVLSQGERTALLN
                     FLVAPDGQRLFATNCSPCHGRSVAVSGGESELRTLISQGGLHLEMPPWREQLSDTELD
                     SLARYVVDPGSAPESEGLFGTYCASCHGERVPTSEDVDQAREIIASGGSHETMPVWGA
                     VLTPEQLDALVSYTLSAAKGTSLDVGQELFSTNCAACHGDFGEGGANPTRSNDIIAPI
                     STSEYLKTRDDFTLRAIIAQGQPNFGMSPFSSSFGGPLDDEEIDAIVAYMRSWEENPP
                     VELPPEVVPTQVSISETDIYANLCAQCHGPNGEGGLGPSISDPEFQAAYTDQEIFDAI
                     NRGHKATSMIGWGEVLSADQIQKLVGFIRKLQPAAEQGPAPGASTVPSATPSPGAAPA
                     AVSFSQDVLPIFEAKCTMCHGVLGGWDGTSYEAVINTGNNAPVVIPGDPENSLLAQKL
                     LGTQTEGAIMPPAGKLPDAEIQIILDWIAAGALEE"
     gene            2787..4964
                     /locus_tag="AC16V7_09260"
     CDS             2787..4964
                     /locus_tag="AC16V7_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257349.1"
                     /translation="MKSKYLLMGVALLALAMVIAACAQAPAPVETTPCPEAPPCPDCP
                     APPEPPSCPEAVVKDVPFEAEWVGSPHNDTEAEAFNHWNEDDPQEVPTSCARCHSTPG
                     YQDYLGADGSAVGEVDQAAPIGTTIQCVACHNAATATLSSVTFPSGVEVTGLGREARC
                     MVCHQGRASKVQVDSSLEQAGLLEDLDTVNAELGFINIHYYAAGATLYGGVTMGGYQY
                     EGKMYDAKFRHVEGIDTCVGCHNPHTTELKLDVCAGCHEGVAAKEDLKKVRMNGSLAD
                     YDGDGDTSEGVAEEMDGLRAMLLTAIQAYASEVAGAPIGYNSAAYPYFFIDTNADGEI
                     SDDEAVSDNRYVSWTGRLLRAGYNYQVSVKDPGAHAHGGKYIIQLLYDSIEDLNTALS
                     SPVDLSTAHREDPGHFAGDTEPFRHWDEEGEVPGSCARCHSATGLRTYIQEGVNVAAE
                     IANGFMCVTCHNEEEWPARYAVNEVTFPSGATLTFGEGKESNLCIVCHQGRSSTPTVN
                     RALGDKPADTVDDTIRFSNIHYFAAGATIFGNDAQGAYVFDGKEYVGPHPHVAQAGFE
                     CKSCHDVHALDVKVETCAGCHGGSSDPTDPDTYRIDATDWDGDGDTTEGVKSEIASFG
                     ERLYAGLQAYAKDKGTPLVYDSHSYPYFFVDADENGEPDANAEGGLVGYNAWTPNLLK
                     SAYNYQYWQKDPGNFAHNAKFMLQILYDSIENVGGDVAGLTRP"
BASE COUNT         1131 a         1314 c         1421 g         1149 t
ORIGIN      
        1 cctgatcatg cctatgatag tgttcagtac attaccaccc aggttccgat gggatggtta
       61 atccgcggct tacaccattg gggtgcaagt gccatggtgg ttgtggttgt actgcacatg
      121 ctgcgcatta tcttctatgg cgcctataaa ttccctagag aggtgacctg gttcacgggc
      181 gtgattttac taattgtgac gattgggttt ggttttaccg gttacctact gccttgggat
      241 cagaaagcat actgggctac aaccgtaggc acgcggattg caggaacccc gccagtgatt
      301 ggtgagtgga tattgctgat cattcgcggt ggtgaggagc tctctgcagt tactttagcg
      361 cgtttctatg gtgtgcatat ctgggttttg cctaccgttc tgttgttgat caccttgatc
      421 catttatacc tggtgatccg tatcggtatc agtgctgtcc ctgggcgcga ggaataagta
      481 atggaagaaa aagaaaaaca acaatattac gaaaagtatc gaaaggccaa ggaaaaaggt
      541 atattctttt atccggatgc catcttcaaa gatgcagtcg ttgtctttgt aattttcctg
      601 atcctcatcg ggctggccta ttttattgga gcgccgctgg aagagcgcgc aaatccagcc
      661 gatacctcct acacgccccg cccagagtgg tatttcctgt tcttgttcca gctgctgaaa
      721 tactttccgg gcgaactcga ggtcttgggt gtggtggtat tgcctaccct ggcaatcata
      781 ctgttatttg cgctgccatt tctagaccgg tcatccaggc ggcattttcg tagccggcca
      841 ttgttcgtgg gtgtgacggc tttgatgatg atcggggttg ttgctttgac agtccagtcc
      901 tatcgagaag ccccacctcc ggcagaagag attgctggtg atcaaacggc tgcgctctac
      961 gctaaaaact gcgctggttg tcacgggcca tctattgatg ttgcagcggg cacaaacctg
     1021 catgccatta ttgcacaagg caagcatgaa ggaatgcctg cctggagtgc ggatctcacc
     1081 tcggatcaaa tcgatgccct ggctggcttc gtcctctctc cgggggggag caagctcttt
     1141 acggcgaact gcggtgaatg ccatagcgca gcggacatgg ttgcgggcga tccaatcgaa
     1201 cttaaaaagg ccatcgagca aggtaaagaa tacccagttc acgcggatgt ggatattccc
     1261 ccatggaatg aggtcttgag ccagggcgag cgaacagcct tgcttaactt cctggttgca
     1321 ccagatggtc agcggctatt tgcgaccaac tgctctccct gtcatggtcg ctcggttgca
     1381 gtttcgggtg gagaaagcga actgcgcacc ttgatcagcc agggcgggct gcatctggag
     1441 atgccacctt ggcgggagca attgagtgat accgagttag acagtctggc aaggtacgtg
     1501 gtcgatccag gctcggcacc tgaatcggag ggattgttcg gcacttattg tgcttcctgc
     1561 cacggagagc gtgttcctac aagcgaagat gttgatcagg cgcgtgagat tatcgccagc
     1621 ggcggctctc atgagaccat gccggtttgg ggggctgtat taacccctga gcagctggat
     1681 gccctggtaa gctacaccct gtctgccgct aaagggacat ccttggatgt ggggcaggaa
     1741 ttattctcca caaactgtgc agcttgccat ggcgactttg gcgaaggggg tgccaatcca
     1801 acccgctcga atgacatcat tgccccgatt agcaccagcg aatacttgaa gacgcgtgat
     1861 gacttcacac tgcgggcaat catcgcccag ggacagccca attttggcat gtcgcccttc
     1921 agtagcagtt tcggtgggcc gctggatgac gaagagatcg atgccatcgt ggcgtatatg
     1981 cgctcttggg aagagaaccc gccggtggag cttccccctg aagttgtccc aacccaagta
     2041 tcgatcagtg aaacagatat ctatgctaat ctgtgtgcgc aatgccatgg tccaaatgga
     2101 gagggtgggc ttggaccttc tatcagtgat ccggaattcc aggctgcata cacagatcag
     2161 gaaattttcg atgccatcaa tagaggtcac aaagccacat ccatgatcgg atggggcgag
     2221 gtactgagtg cagatcaaat tcagaaactg gtaggcttta tccggaagtt gcaacccgct
     2281 gctgagcagg gacctgcacc aggtgcatcc acagtcccaa gtgcaacccc aagccctggc
     2341 gcggctccgg ctgcagtatc tttctcccag gatgttttgc ccatttttga ggcgaaatgc
     2401 accatgtgtc acggtgtttt aggtggttgg gatggtacca gctacgaggc tgtgataaac
     2461 accgggaata acgctccggt agttataccg ggcgatcctg aaaatagcct gttggcgcag
     2521 aagctgctcg gcacacaaac cgaaggtgcc atcatgccac ccgcagggaa attgcctgac
     2581 gccgaaatcc agatcatcct ggactggatt gcggctggcg ccctggagga gtagtcgacc
     2641 cttcatgtag cttgtcaccc atcagggtcg ttacgcccac tacttggata ataagacttt
     2701 ggagaatcaa ggaggtgaga caagagaaaa atacataaag aatacgtcct gaccaatgag
     2761 tttcatcatc agagaggagt atgtatatga aatccaagta tctgcttatg ggagttgcgc
     2821 tgctagccct ggcaatggtc atagcagcct gcgcccaagc acctgctccg gttgagacga
     2881 cgccttgtcc agaggcgccg ccttgtccgg attgcccggc tccaccagaa cctccatctt
     2941 gcccggaggc tgtggtgaag gatgtcccat tcgaagccga gtgggttggc tcgccacaca
     3001 atgatacaga ggcagaggcc ttcaatcact ggaatgagga cgaccctcag gaagtcccca
     3061 cctcctgtgc aagatgccac agcacgcctg gctaccagga ttacctgggc gctgatggat
     3121 ctgcggtagg cgaggtggat caagcagccc caatcggcac gactattcag tgtgtggctt
     3181 gccacaacgc ggctaccgcg actctgtcca gcgtgacatt cccatctggc gtggaagtta
     3241 ccgggctggg gcgtgaagcg cgctgcatgg tctgccatca gggtcgcgct tccaaagtcc
     3301 aggtggattc ttccctggag caagctggct tgcttgaaga cctggataca gtgaacgccg
     3361 aactgggctt catcaatatc cactactacg cggctggtgc cactctgtac ggcggtgtga
     3421 ctatgggcgg ctaccaatac gaaggcaaga tgtacgatgc caagttccgc catgtggaag
     3481 gtatcgacac ttgcgtaggt tgccataacc cacacaccac tgagctaaag ctggatgtgt
     3541 gcgctggatg ccatgagggc gtagctgcta aggaggacct gaagaaagtg cgcatgaatg
     3601 gttctctggc tgactacgat ggcgatggtg ataccagcga aggcgttgca gaagagatgg
     3661 acgggctgcg cgccatgctg ttgaccgcta ttcaggcgta tgccagcgag gtagctggtg
     3721 ctcccattgg ctacaactcg gcggcttacc cgtacttctt cattgacacc aacgcagacg
     3781 gcgagatcag cgacgacgaa gctgtatctg acaatagata tgtctcctgg accggtcgtt
     3841 tgctgcgagc tggttacaac taccaagtgt cggtgaagga tcccggtgcg cacgctcatg
     3901 gcggtaagta catcatccaa ctgctctacg actcgatcga ggacctgaac actgccctga
     3961 gctcaccggt ggacctgtct acagcacacc gtgaggatcc cggtcacttt gccggcgaca
     4021 ccgagccatt ccgccattgg gatgaagagg gtgaggtacc tggaagttgt gcacggtgcc
     4081 acagcgctac cggcttgcgt acctacattc aagaaggtgt gaatgtcgcg gccgaaatag
     4141 ccaacggttt catgtgcgtc acctgccaca acgaggaaga atggcctgcc cgctatgcag
     4201 tcaatgaggt caccttcccc agcggcgcca cgctgacctt cggcgagggc aaggagtcca
     4261 acctgtgcat tgtatgccac cagggacgct cctctactcc cacggtcaat cgggcgcttg
     4321 gagataaacc tgccgacacg gtggatgaca cgatcagatt ctccaacatc cactacttcg
     4381 cggcgggcgc tacaatcttt ggcaatgatg cccagggcgc gtacgtgttt gacggcaaag
     4441 aatacgttgg gccgcatccc catgtggcac aggctggctt tgagtgcaaa tcctgccatg
     4501 acgtccatgc tttggatgtc aaggttgaaa cttgcgccgg ctgccatggc ggctcatcag
     4561 acccgacaga tcccgacacc taccgcattg acgcaacgga ctgggacggc gatggcgata
     4621 ccaccgaggg tgtaaagagt gagatcgcta gcttcggtga gcgtctgtat gctggtctcc
     4681 aggcctatgc caaggataag ggcaccccgc tcgtttacga ctcccacagc tatccgtact
     4741 tcttcgtcga tgcagacgag aacggtgagc cggacgcaaa tgccgaggga ggcctggtag
     4801 gctacaatgc ctggacgccc aacttgctca agtctgccta caactaccag tactggcaga
     4861 aggatccggg caacttcgcc cacaatgcca agttcatgct gcagatcttg tatgacagca
     4921 tcgagaacgt tggtggtgat gtcgctggct tgacaagacc ttagtccata aatatgacac
     4981 atcccgaata actttttcgg gttgagtcgt gtctt
//
LOCUS       DBNHOH010000531         1673 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1569956, whole genome shotgun sequence.
ACCESSION   DBNHOH010000531 DBNHOH010000000
VERSION     DBNHOH010000531.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1569956"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..1261
                     /locus_tag="AC16V7_09265"
     CDS             203..1261
                     /locus_tag="AC16V7_09265"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HIR6257350.1"
                     /translation="MEHVRVGVIGTGRMGQRHCRIFSNLRKVQLVGVYDANPVVGYKV
                     AQQYDAPFFDNIDDLLKQVDAVSLATPTFFHHEQAMLCISKGLHVLVEKPITRTLDQA
                     EALVKAAEESELVVQVGHIERFNPAYIELKNILEEYQPLSINIQRLSPYQGSNKDVDV
                     VMDLMIHDANLMLDLLKKNPTKMSAYGLTAFSGTIDHAIAQLIFEGGPMLTMTSSRLT
                     EEKIRKIDVTCREAYIECDLLNKSISVHRSTFGEYLNSNRRGVKYRQEGIIERINIPS
                     FEPLFLQLQHFVDCILEEKTPYVTAQDGYDALKLTLYIRDCICEHLINMDRRKTPRVD
                     TPSIMIEPSVNVSSPRPN"
BASE COUNT          454 a          397 c          406 g          416 t
ORIGIN      
        1 aggcttgcac agtggcgagg tctaagggga tagtcttaag caacgtattc catgaccgcc
       61 ggatttcctg gttgaccttg caagttgtgc cgacctgcct tgtctcttgc acacaaccat
      121 aagtgcctgg cggtccattg ggttaccaaa ggcggggtgg tcattcaagc ctcttctttt
      181 gttgatgaaa ggaaagggca ttatggaaca tgttcgagta ggagtaattg gtacagggcg
      241 catgggccag agacactgcc gcattttctc gaatttgcgg aaagtgcaac tggtcggcgt
      301 atatgatgcc aaccctgtag tgggttataa ggtggctcaa caatacgatg cgcccttttt
      361 tgataacatt gacgatttgc taaaacaggt ggacgcagtc agcctggcga cgcccacctt
      421 cttccaccat gaacaggcca tgctatgtat ctcaaaaggg ttgcacgtct tggtggaaaa
      481 gccaatcaca agaaccttag accaggcaga agccttagta aaggcagccg aagaaagtga
      541 gttggttgtt caggttgggc acatcgagcg cttcaaccct gcgtatattg aacttaagaa
      601 catcttagaa gaataccaac ctctctcgat taacatacaa cgcctaagcc cataccaggg
      661 aagcaacaag gatgtggatg ttgtgatgga tctgatgatc catgacgcca acctgatgct
      721 ggatctgctg aagaaaaatc cgacgaaaat gagcgcttat ggcttgacgg cattcagcgg
      781 tacgattgac cacgccatcg cccaactgat ttttgaaggc gggcctatgc taacgatgac
      841 gtcctcgcgc ctcaccgagg agaagatccg caaaatcgat gtcacctgcc gggaagcata
      901 tatcgagtgc gatctgctca acaagagcat atctgtccac cgcagcacat ttggagaata
      961 cctgaattcc aaccgtagag gggtgaagta tcgccaggag gggatcattg aacggatcaa
     1021 catcccttcg tttgagcctc tcttcctgca attgcagcac tttgtggatt gcatcctgga
     1081 ggagaaaacc ccctatgtga ccgcccagga cggatatgat gccttgaagc tgaccttata
     1141 catccgagac tgcatctgcg agcatttgat caacatggat cgtagaaaaa caccccgcgt
     1201 cgacactccc tcgattatga tcgagccctc ggttaatgtg agctcgccga gacccaatta
     1261 gtgcagcatg ccatcagacg aatcacagag cctgggaacc caggctctgt tttttttacg
     1321 ccatggatta atgtccaata gatacaatgt cttcttcttg acagcaagaa tagccaggct
     1381 ggtatttctc ttcattaata acctgagcct gagataatcg tgtatttggg gtgttattgg
     1441 gcgggtcatg acccgcccaa actccccctc ttccctcttc aactgcgtaa gtcctaatta
     1501 aattaaagct tacaatgtac ttataatatt gaaaaacgct tgaaatcaga taagaagggt
     1561 ggtaaaatca gggcaagtgt attaagctat tgttaagtcg gggtagttca atcaagcatc
     1621 gatccaaaac ggatttcaaa gccatcccca tatgagcgat tttattggat tct
//
LOCUS       DBNHOH010000532         2534 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1185562, whole genome shotgun sequence.
ACCESSION   DBNHOH010000532 DBNHOH010000000
VERSION     DBNHOH010000532.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1185562"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="AC16V7_09270"
     CDS             <1..594
                     /locus_tag="AC16V7_09270"
                     /inference="COORDINATES: protein motif:HMM:NF014049.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HIR6257351.1"
                     /translation="SNQYAKGSRRDWGQVLANGGFGAVLVVVGYFFPGDAWPWVAYGG
                     ALAAVNADTWATELGVFNPSPPRLITNGRPVERGTSGGVTMLGYLAVVGGAGLIGSLA
                     VILASSQPMNSGMLAGVITLAGMVGSTFDSLLGATVQAIYYCPQCGKETERHPLHSCG
                     THTTLSRGWRWLNNDLVNFFCSLAGAITAWSLWAFLA"
     gene            complement(609..1364)
                     /locus_tag="AC16V7_09275"
     CDS             complement(609..1364)
                     /locus_tag="AC16V7_09275"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6257352.1"
                     /translation="MPSSHQIKAILFDLDGTLRHNRPSYTDVFFDISSRLGAPSVPEA
                     QYRAKRWMHYYWAKSDELLSDRRMYDDQTEAFYINHARLVLIAMGCTPDLAAALAPKA
                     YQMMKDEFKPQDWVPPDVHLTLDELKAAKYPLAVLSNRLRPFHEQMETLGLTPYFEFA
                     LHAGEAKSWKPDPQVFHQAVERLGYSPYETLYVGDNYFADVVGARRANLVPVLIDPEG
                     LFPEADCAVIQQFGDLKELLLQQENLNTSIPQE"
     gene            complement(1501..2481)
                     /gene="moaA"
                     /locus_tag="AC16V7_09280"
     CDS             complement(1501..2481)
                     /gene="moaA"
                     /locus_tag="AC16V7_09280"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HIR6257353.1"
                     /translation="MALDRFGRSIHYLRISLTDHCNLRCVYCMPEDMTFRPNAELMQD
                     DELILLVRLFASLGFDKMRLTGGEPTVRLHIVELVHAIARTPGVRSLSMTTNGILLSR
                     LARPLAEAGLQRVNISLDTLNPDKFRRVTRWGKLDDVWNGIMAAEEAGLTPVKLNAVI
                     VRGYNEPDIVDLARLTLERPWQVRFIEMMPFAGVTDLQTNNVVTAAETQATIEAALGS
                     LEVSNNGELDGEARLFRLPGAPGEIGFISSVSVPFCAAGTRARLTADGRLRLCLLREG
                     EVDLLTPLRAGATLDDLRQLILKGIWHKPWGHGLAEGIIPLNRVMSEIGG"
BASE COUNT          553 a          651 c          770 g          560 t
ORIGIN      
        1 tctaaccaat atgccaaagg ctcgcggcgc gattggggtc aggtgctggc gaatggtgga
       61 tttggggctg tattggttgt ggtaggctat ttttttcctg gcgatgcttg gccttgggtg
      121 gcgtatggcg gtgcgctggc cgcagtgaac gccgatacat gggccactga gctgggtgtc
      181 ttcaaccctt ctccgccgcg cttgatcacg aatggcagac cggttgaacg aggcacttca
      241 ggtggggtga ccatgctagg gtacctggct gttgtgggcg gtgcggggtt gatcggaagc
      301 ctggcagtga ttcttgcgag tagtcaaccg atgaactcag gcatgttggc aggggtgatc
      361 accctggcgg ggatggtggg atcgaccttc gattcgctgc ttggcgcaac cgtccaagcc
      421 atctattatt gcccgcagtg cggaaaagaa accgagcgcc acccccttca tagctgcggg
      481 acgcacacca ccctgagccg cggctggcgc tggctaaata acgacctggt aaatttcttc
      541 tgttcgctgg cgggggcgat caccgcctgg agcttgtggg ctttcctcgc gtaaggttac
      601 actcccgatt attcctgggg gatactggta ttgagatttt cctgctgcag gagcaactcc
      661 ttaaggtctc caaactgttg gattacggcg caatcagcct cggggaacag accttcgggg
      721 tcaatcagca cgggtactag gttggcgcgc cgggcgccaa ccacatccgc gaagtagtta
      781 tcacccacat acagggtctc atagggtgag taccccaggc gctcgacagc ctgatggaag
      841 acctgtggat ctggtttcca ggacttagcc tccccagcgt ggagagcaaa ctcgaagtaa
      901 ggagttagtc ccaaggtttc catttgctca tgaaagggac gtaatcggtt agagagaaca
      961 gcaagggggt attttgctgc tttaagttcg tcaagagtca ggtggacatc tgggggaacc
     1021 caatcttgcg gtttgaactc gtccttcatc atctgatagg cctttggcgc tagcgcagca
     1081 gcaagatcag gcgtgcagcc catagcaatc aagaccagcc gggcatggtt tatatagaaa
     1141 gcttcggttt ggtcgtcata catgcgccgg tcggagagca gctcgtctga tttagcccaa
     1201 taataatgca tccagcgttt ggcgcgatat tgggcctcag gtacagacgg cgcacctaag
     1261 cgggaggaga tgtcgaagaa gacatccgtg taggaaggtc ggttatggcg tagcgttcca
     1321 tccaagtcga acaggatggc tttaatttga tgagaagagg gcaaacaagg cctccttaaa
     1381 gttgtttatg aagcagagag agaaacggag acaaaaactg gaggattgtg ggcggcggaa
     1441 gatgttcggc gatatatagc ttgagtgaga ttagcttccg ctctgcgttt tatccttcgt
     1501 ctaccccccg atttcactca tcacgcggtt caggggaatt ataccctctg ccagaccatg
     1561 cccccagggt ttgtgccaga tgcctttcag gataagctgg cgcaggtcat cgagcgtggc
     1621 acccgcacgc agcggtgtca gcaggtctac ctcaccctcg cgcaacaaac acagtcgcag
     1681 gcgaccgtcg gcggtcaggc gggcgcgcgt gccagcagcg cagaaaggga cgctgacaga
     1741 tgagataaat ccaatttcac ctggggctcc cggcaggcgg aagaggcgcg cttctccatc
     1801 cagttcgccg ttgttggata cctccagcga gcctagggcg gcttcaatag ttgcctgagt
     1861 ctcggcggcg gtgactacgt tgttagtctg caagtcggtt acgcctgcga agggcatcat
     1921 ctcgatgaag cgcacttgcc aggggcgctc aagcgtgagg cgagctaaat caacaatgtc
     1981 gggctcgttg tagccgcgca cgatgacagc attcagcttt accggagtga gacccgcttc
     2041 ttcggcagcc attatcccat tccatacgtc atccagtttg ccccagcgag tgacacgccg
     2101 gaatttatcc ggatttagcg tgtccaggct gatattcacg cgctgcaagc cagcctccgc
     2161 aagggggcgc gccaggcgcg aaagcaagat cccgttggtg gtcatggaca gcgagcgcac
     2221 ccccggcgtg cgggcgatgg cgtgcaccag ttcgacgatg tgaaggcgca cagttggctc
     2281 accgcctgtc aggcgcatct tgtcgaagcc caggctggca aagaggcgca ccaataggat
     2341 gagctcatca tcttgcatca gctcagcgtt ggggcggaag gtcatatctt ccggcatgca
     2401 ataaacgcag cgcaggttgc agtgatcagt caggctgatg cgtaagtagt ggatggagcg
     2461 tccaaagcgg tcgagagcca tagcagatta atttagacta attttatcaa agttatacca
     2521 caaattcagt gttt
//
LOCUS       DBNHOH010000533         1374 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_480419, whole genome shotgun sequence.
ACCESSION   DBNHOH010000533 DBNHOH010000000
VERSION     DBNHOH010000533.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_480419"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="AC16V7_09285"
     CDS             complement(<1..1052)
                     /locus_tag="AC16V7_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR6257354.1"
                     /translation="MSQNVIRVVGARVHNLKNITVEIPRDKLVVITGLSGSGKSSLAF
                     DTIFAEGQRRYVESLSAYARQFLGQMDKPDVDYIDGLSPAVSIDQKGASHNPRSTVGT
                     VTEIYDYLRLLFARAGIPHCPICGRQVERQSAQEIVDAIEALPESSRLLILSPLVRGR
                     KGTHQAVLEEIRKSGFVRARVDGQVHELEDDIQMDRYKIHTIEAVVDRLIIRNPENGD
                     DAQAFRSRLTDSVETALKFGEGYLTVHLTTSLPSPVSQAAIEGRGEMGLGEMVAEERD
                     LHFSEHLACPEHGSVLPEIEPRTFSFNTPHGACPDCQGLGGKLEIDPDLLIPDPHLSL
                     SEGAIAISEWSGPREEG"
BASE COUNT          308 a          371 c          385 g          310 t
ORIGIN      
        1 ccttcttcgc gcgggccgct ccattcagag atagcgatgg caccttcact caaggaaagg
       61 tgcgggtcgg ggataagcag gtcggggtcg atctccagct tgccacccag cccctggcag
      121 tcaggacagg cgccgtgggg ggtattgaag gagaaggtgc gcggctcgat ctctggtaac
      181 accgagccat gttccgggca ggccagatgc tcggagaagt gcaaatcacg ctcctcggca
      241 accatttccc cgagtcccat ttcccctctc ccttcgatag cggcctgaga tacgggagac
      301 gggagcgagg tggtgagatg cacagtcaga tagccctctc caaactttaa tgctgtctca
      361 accgagtcgg tcagccgaga gcgaaaggcc tgcgcgtcat caccgttttc tgggttgcga
      421 atgatcaaac gatccaccac agcttcgata gtgtggatct tgtagcgatc catctggata
      481 tcgtcttcta gctcgtgcac ttgcccgtcc actcgagcgc gcacgaagcc agacttgcga
      541 atctcttcca gcacggcctg gtgagtgccc ttacgtcctc gcaccagcgg cgagagaatc
      601 agcaatcggg agctctcggg cagtgcttcg atggcatcca caatctcctg ggcagactgg
      661 cgttccacct gccgcccaca aatcgggcaa tgcgggatgc ccgcgcgggc aaaaagcagg
      721 cgcaagtaat cgtagatctc ggtgaccgtg cctaccgttg agcgggggtt gtgcgaggcg
      781 cccttctggt cgatggagac cgccggggag aggccatcga tgtaatcaac atccggcttg
      841 tccatctgcc ccaggaactg gcgggcatat gccgagagcg attcgacgta gcgccgctga
      901 ccttcagcga agatggtatc gaatgccaga gaggatttcc ctgaacccga gagtccagtg
      961 atgactacca gtttatcgcg agggatttca accgtgatat tttttaagtt atgaacgcgt
     1021 gcccctacaa cacgaatgac attttgagac atagccaata ttttagcaca tttgttttat
     1081 tattcacaga tattttcatc ataagttacg cagttcgaga ggtgatgggg gagtttgggg
     1141 tgtgctggca gcaggctttg ctacttggga gcgcacccaa ctcttccttt cgacaaaagc
     1201 ttattatacc aaagccatca gaactgctct ggtaaaatta tcacattcac agggtaattg
     1261 catatgccct gaggggcatg tgcaatcccg caaaaaaagt gtttggggtg ttttggtggg
     1321 ggtatgcctc caccaaaaca ccctatattc tctcctcagt ggtgggaaat cgcc
//
LOCUS       DBNHOH010000534         2288 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_351907, whole genome shotgun sequence.
ACCESSION   DBNHOH010000534 DBNHOH010000000
VERSION     DBNHOH010000534.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_351907"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="AC16V7_09290"
     CDS             <1..971
                     /locus_tag="AC16V7_09290"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HIR6257355.1"
                     /translation="PAHRAIDAAQEAARGKGAIGTTLRGIGPAYTDKVARSGLRLGDV
                     LDSRSFVPALHSHIEAANQRLTQLYHAEPLDVAATTHEYIQFTQQLQPYIQDVSALLS
                     KALKDGQRVLAEGAQGTLLDLDHGSYPFVTSSYPSAAGALLGLGLGVGFVERVIGVTK
                     AFQTRVGAGPFPTELSGDLALHLRGTGEQPWDEYGTTTGRPRRVGWLDMVLLRYALRI
                     NGLTELALTKLDIPTGLEPLRICTAYRKGGQVYKDLPLGPGELAGFEPVYEELPGWQN
                     DLRSTRTWEELPPQSRAYILRIEELSSVPVRTVSVGPERQQVVEIS"
     gene            979..1740
                     /locus_tag="AC16V7_09295"
     CDS             979..1740
                     /locus_tag="AC16V7_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257356.1"
                     /translation="MLKYTLWLVLSMILAGCLSRPTALPPEPTSTATPTGTPTATVIW
                     FPATATQTPLPTLDVSPTVDQLPPTGDLIFSDDFNDPDEWLLGQTSTTSIALGNQALT
                     LALNQPSGYLYTLRKSPILSDFYLEVTASPSLCRGDDEYGLLLRVSPSVEFYRFSLSC
                     EGSIRLDKYYNGKASSPQAKTPSGSVPRGAPSSSLISAWVKGKEMQFYVNNEYQFTVR
                     DPSLQEGSLGFFVRSAGDTALTVSFTNLSVRTIPN"
     gene            1750..>2288
                     /locus_tag="AC16V7_09300"
     CDS             1750..>2288
                     /locus_tag="AC16V7_09300"
                     /inference="COORDINATES: protein motif:HMM:NF013704.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HIR6257357.1"
                     /translation="MAKRRLVNRLNDLSPKEWLKFQKSWFVHNPPPRRKDVLRHPAKF
                     PETLAQEFIEFFTRRGDVVLDPMVGTGSAMVAALRSGRHSYGVELNPAYAAIAQQVVS
                     EEQALLGEQARDLTAEVIVGNAADIAHYADTLQIPPADYVLTSPPYWDMLRARGAQTQ
                     KNRRASPELDVFYSDDPADL"
BASE COUNT          498 a          707 c          610 g          473 t
ORIGIN      
        1 cacctgccca ccgtgctatc gacgctgctc aagaagcggc gcgtggcaaa ggcgcaattg
       61 gtactacctt gcgcggcatc ggtccagctt atactgataa agtggcgcgc agcgggctgc
      121 gcctgggtga cgtgttggat agccgctctt ttgttccagc tttgcattct cacattgaag
      181 cagctaacca gcgcctgacc caactatatc atgctgaacc actggatgtt gccgctacga
      241 cacacgaata tatccaattt acccaacaac ttcagcccta cattcaagac gtgtctgcgc
      301 tgctctccaa agcactgaag gatggtcagc gtgtcctggc ggaaggcgcc caaggcaccc
      361 tgctcgacct ggatcacggc tcatatccat ttgtcaccag ttcgtatccc tccgcagctg
      421 gcgccttact ggggcttggc ctgggggttg gcttcgtcga gcgcgtcatt ggggttacta
      481 aagctttcca aacacgcgtg ggtgcaggtc cattccccac agagctctcc ggcgacctgg
      541 ccttgcactt gcgcggcacc ggggaacagc cctgggacga atatggcact accaccggca
      601 gaccgcggcg ggtaggctgg ctggatatgg tgctgctgcg ctacgcgttg cgcattaatg
      661 gccttaccga gctagcactg accaagctgg atatccccac cggcttggag ccgctacgca
      721 tttgcactgc ttaccgcaag ggaggacagg tatacaagga tctaccctta ggacctggtg
      781 aactggcggg ctttgaaccg gtatacgaag agctacccgg ctggcagaat gacttgcgct
      841 ccacacgcac ctgggaagaa ctacctcccc agtcacgagc ctacatcctg cgcatcgagg
      901 agttgagcag cgtgccggtg cgcacggttt ccgttggacc agagcgtcag caagtggtcg
      961 agatcagcta agggttgaat gttgaaatat acactctggc tcgtcctgtc catgattctg
     1021 gcggggtgcc tgtcgcgccc cacagcactt ccacccgagc cgaccagcac ggcgactcca
     1081 accggaacac caaccgccac ggtaatttgg tttcccgcca cagccaccca gacgccgctc
     1141 ccaaccttgg atgtatcacc cactgttgac cagcttccgc caacaggcga tctgatcttc
     1201 agcgacgact tcaacgatcc ggacgagtgg ttattggggc agacttccac caccagcatc
     1261 gccctgggta atcaagctct aaccctggcg ctcaaccaac cgagtggata cctgtacaca
     1321 ctgaggaaaa gccccatcct atcggatttt tatctagaag tcacggcaag ccccagcctg
     1381 tgccgaggcg atgatgaata cggcttgctg ctgcgcgtct ccccttcagt agaattctac
     1441 cgtttctccc tctcctgcga aggaagcata cgcctagaca agtattacaa cgggaaagca
     1501 tcttcccccc aagcaaaaac ccccagtggc tctgtcccgc ggggcgcgcc aagcagctct
     1561 ctgatcagcg cgtgggtaaa gggcaaagag atgcagtttt acgtcaacaa tgaatatcaa
     1621 tttactgtca gagacccttc gctccaggaa ggcagcctgg gtttctttgt gcgctcggca
     1681 ggtgataccg cattgacagt cagctttacc aatttatcgg tgcggacaat ccccaactaa
     1741 ggaggtagca tggcaaagcg gcggctcgtt aaccgtctga acgaccttag ccccaaggaa
     1801 tggctcaagt ttcaaaagtc atggtttgtt cacaacccgc caccgcgccg caaggacgtg
     1861 ttgcgccacc cggccaagtt tccggagaca ttggcgcaag agttcattga gttcttcacc
     1921 cggcgcggcg atgtggttct cgaccccatg gtaggcacgg gtagcgctat ggtagctgcc
     1981 ctgcgcagcg gcaggcacag ctacggcgtc gagctgaatc ccgcctatgc tgccatcgct
     2041 cagcaagtgg tcagcgaaga gcaggctttg ctgggtgagc aggcaagaga tctgaccgcc
     2101 gaggttatcg tgggcaacgc cgcagatatt gcgcactatg ccgacactct ccaaattccc
     2161 ccagcagatt atgtcctgac ctccccacct tattgggaca tgctacgcgc tcgcggcgcg
     2221 cagacacaga aaaatcgccg cgcctccccc gagctagatg tattctactc cgatgaccct
     2281 gctgacct
//
LOCUS       DBNHOH010000535         2099 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_95647, whole genome shotgun sequence.
ACCESSION   DBNHOH010000535 DBNHOH010000000
VERSION     DBNHOH010000535.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_95647"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="AC16V7_09305"
     CDS             <1..285
                     /locus_tag="AC16V7_09305"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014326.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HIR6257358.1"
                     /translation="AAEHARTGEWDEAQAALDEANEEQLKAHQINADIIRLEASDQAV
                     PFSVLLVHAMDLLLLAWSELDYTEQFIKMYQRMDALEAEVAQWRNQASKS"
     gene            258..1565
                     /locus_tag="AC16V7_09310"
     CDS             258..1565
                     /locus_tag="AC16V7_09310"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HIR6257359.1"
                     /translation="MAQPSIEKLTVIGGGSSYTPELIDGFIQNEDKVRVGEIVLFDVD
                     QERLKIVGGMAQRMAQYAELETKITLSHDRPASVEGAQFVLSSMRVGQMPARIRDERI
                     PLKYGVIGQETTGPGGTLKAWRTIPVTLDVARDMARYAPESWYLNFTNPSGIITEAIL
                     KHTDIKVVGLCNNPINTQAGMAEFFGVEPQGVFLEWVGLNHVNWVRRVYVRGKDVTSD
                     IMAGLEGLSQLEDLPHFDPELVRTLGVLPTYYLEYYYNHPRKLSEARTSEKTRGEVVR
                     EVEEALMKKYADPNQVVVPPELSKRGGARYSEAAINLILSLMLDRRDIQIVVTRNGTS
                     LPDLPPDASVEVPCVVGSHGVTPLSMGPLPESIRSLCQQAKAWESATVKAGVSGSRRD
                     ARLAMLLNPLVPSYEVAAALVDEMLEANRDYLPQFYGDKNDKN"
     gene            1552..>2099
                     /locus_tag="AC16V7_09315"
     CDS             1552..>2099
                     /locus_tag="AC16V7_09315"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR6257360.1"
                     /translation="MTKIDLTLPVEIPEWLQIARRPVEVGEGHLERQYQEIADEVRAA
                     VDAVLPTGKYTLGPHLAQFEKDFAAYCDCKYSIGISSGTAALHLALEALDFGPGDEVI
                     TVSNTYVATAFAVSYTGATPVFVDVDPKTFNMTAELVEAKLTPRTRAIIPVHLYGHPV
                     DMDPIMELAKQHDLWVLEDAAHS"
BASE COUNT          456 a          589 c          611 g          443 t
ORIGIN      
        1 gctgcagagc acgcccggac tggcgaatgg gatgaagctc aagctgcgct ggatgaagcc
       61 aacgaggagc aactcaaggc gcatcaaatt aatgcagaca tcatacgcct ggaagccagc
      121 gatcaggccg tccctttctc agtcctgctg gtgcatgcca tggacttgtt gctactagcc
      181 tggagtgagc tggattacac cgaacaattt atcaagatgt atcagcgtat ggatgcgctg
      241 gaagcggagg tcgcgcaatg gcgcaaccaa gcatcgaaaa gctgaccgtc attggcggcg
      301 ggagctccta tactcccgag ctgattgatg gcttcattca aaatgaagac aaggtgcgcg
      361 tcggcgagat cgttcttttc gatgttgacc aggagcgttt gaaaatcgtg ggtggcatgg
      421 cgcagcgtat ggcgcagtat gctgagcttg agaccaagat cacgctcagc cacgaccgcc
      481 ctgcttccgt tgagggcgcc caatttgtcc tctcctccat gcgtgtaggg cagatgccag
      541 cccgcatccg tgacgagcgc atccccctta aatacggggt tatcggccag gagaccaccg
      601 gccccggtgg taccctgaag gcctggcgga cgatccctgt taccctggac gtcgcccgtg
      661 acatggcgcg ttatgctccc gaatcgtggt atttaaactt caccaaccct tctggcatca
      721 tcaccgaagc catcttaaaa cacaccgaca tcaaggttgt aggcctgtgt aacaacccca
      781 ttaacacgca agctggcatg gcggaatttt tcggagtgga accgcaaggt gtctttctgg
      841 agtgggttgg actgaatcac gtcaattggg tgcggcgtgt atatgtgcgc ggtaaggatg
      901 tcacctcaga tattatggca ggtctggaag gcttgagtca attggaggat ctgccgcact
      961 ttgaccctga gctggtgcgt accctgggcg tcctcccgac ttattacctg gaatactatt
     1021 acaaccatcc ccgcaagctg tcggaggcgc gcaccagtga aaaaacgcgc ggcgaagtcg
     1081 tgcgcgaggt cgaagaggcg ctgatgaaaa agtacgccga cccgaaccag gtggtagtgc
     1141 cacctgagct ctccaagcgg ggtggcgcgc gctattcgga ggcagcgatc aatttgatcc
     1201 tatcgctcat gcttgaccgt cgcgacattc agatcgtggt cacccgcaac ggaaccagct
     1261 tgcccgacct gccgccggat gcctccgtag aggtgccatg cgtggtgggc agccacgggg
     1321 tcactccgct gagcatggga ccgctgccag agtcgatccg ctcgctttgc cagcaggcca
     1381 aggcatggga gtcggccacg gtgaaagccg gggtttctgg ctctcgtcgc gatgcccgcc
     1441 tggcgatgtt gctgaacccg cttgtcccgt cttacgaggt cgccgcggcc ctggtggacg
     1501 agatgctcga agctaatcga gattacctgc cgcagttcta tggagataag aatgacaaaa
     1561 attgatctca cgctcccggt cgaaatcccg gagtggctgc aaatcgcccg ccgtccagtc
     1621 gaggtcggcg aaggccatct ggaacgccaa tatcaagaga ttgctgacga ggtgcgcgcc
     1681 gcagtcgatg cggtgctacc cactggaaaa tatactcttg gcccccatct ggctcagttt
     1741 gagaaggact tcgcggctta ttgcgattgc aagtacagca tcggaatatc cagtggtaca
     1801 gctgcattgc acctggcctt agaggctctc gatttcggcc ctggtgacga ggtgatcaca
     1861 gtcagcaaca cgtatgtggc gactgcattt gctgtctcgt atactggtgc cacgccggtt
     1921 ttcgtggatg tggaccccaa gacgttcaat atgaccgccg agctggtcga agcaaagctc
     1981 actccacgta cgcgtgccat cataccagtt cacctctatg gccacccggt ggatatggac
     2041 ccgattatgg agcttgccaa acagcacgac ctgtgggtgc tcgaagacgc tgcacactc
//
LOCUS       DBNHOH010000536         4136 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1634028, whole genome shotgun sequence.
ACCESSION   DBNHOH010000536 DBNHOH010000000
VERSION     DBNHOH010000536.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1634028"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..813
                     /locus_tag="AC16V7_09320"
     CDS             97..813
                     /locus_tag="AC16V7_09320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6257361.1"
                     /translation="MTPDNTITLYKWILKEFTIAERGKAVRKIGRHLAPLIRPADKVL
                     DLCCGTGPASFWFEERGAAVTAIDFAPYMIALARDEASKRNSLVEFIEGDIFIYDFGQ
                     DRYDLVACLGNSISDFSLSAFVALGKKVAKALKPGGRFVLQYHDGFFPFIHKEYVRTG
                     VLQETPERVTFFFKEYLPEKGAYVKIYRNETLGEEYQRIGYIYSVPMVHSAMDGILDI
                     EQHIGLDADQFLDVFTKPGK"
     gene            1040..1495
                     /locus_tag="AC16V7_09325"
     CDS             1040..1495
                     /locus_tag="AC16V7_09325"
                     /inference="COORDINATES: protein motif:HMM:NF033634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HIR6257362.1"
                     /translation="MDNRVEQYASWYDRKSGPIKSRFLYIKAVTVIGGALVPVLVNSN
                     FDFLNVGFDVAKMAATLLSLIVVVLVSWDSVFQYGKQWKNYRSTEQFLRQEVVLFKYR
                     TNFYSGLDDSKAFQIFIERVENAIAQENAVTLDTLTRDSNVGRQGAREE"
     gene            1707..2606
                     /locus_tag="AC16V7_09330"
     CDS             1707..2606
                     /locus_tag="AC16V7_09330"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR6257363.1"
                     /translation="MKARNSTHVENRVAGVAIQSFSKARPILMCVILFIIALVFRLID
                     SIVLRLDELLGELILTKSLGFILVLVWVWFTGRKIRDIGLHSQNLGQSLLIGTLTTLV
                     AFIFGYSVEIAFAIQQGTQPTLQLGAIDPKMGVTGGLLFGLWLLLGNFVNSFMEEGLF
                     RGVMGRLARIRFNFWQTNWFQAFMFGIWHLPWVLKYYLVGEVQTGGEIFTAVVYNSLP
                     QLIIGIVYGYLYLKTNSLWAAWIAHTLSNSASNFLHVNTTAGLDTGLPLRMTVYLVVM
                     LFSLFWVRQVTKRYRMPEVMPWE"
     gene            2629..>4136
                     /locus_tag="AC16V7_09335"
     CDS             2629..>4136
                     /locus_tag="AC16V7_09335"
                     /inference="COORDINATES: protein motif:HMM:NF025253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HIR6257364.1"
                     /translation="MLIRINARTFTLLFLLFFLVGCLARTSEPPRVGGCDDGQTGAPC
                     SVVNGFNGNQVPSDVIGATISGGGGTSFPNTVLLDYGVVGGGKGNLAGNLSAVGGGFS
                     NDASGIRAFIGGGANNTASRDSAVIGGGYGNIADQSYTTISGGELNKTSGSWATIGGG
                     SGNIGNGRYATVGGGTHNLAESAYASIGGGTYNEAGGGNSAIAGGTRNKALGIGSNIG
                     GGSGNRAVGYQSAIGGGLANSATGDYSLVSGGRGNQAGIANSDPEVASYATVGGGYEN
                     IAAGAFSVVPGGLANYALGEYSLAAGNRARVEPDHPGTFVFADSSDYPFSSSSANEFA
                     ARATGGVRFVTAVDESGAPMAGVRLGAGSGAWESLSDRNAKSSIQYVDVRQVLEALVG
                     LPISTWRYRGQDETVRHIGPMAQDFYAVFDLGQDERYISTVDADGVALASIQGLYQLL
                     QGKDAQIIALQAENLSQGQQLADMDARITALEKHSFESGAQINWYLAMGAALL"
BASE COUNT          999 a          879 c         1158 g         1100 t
ORIGIN      
        1 cgcagatagc aattgtgaga taattatctt tggctagctg atacgccagc ttgtagcagt
       61 tgagcggtcg gaattactac cgacgaaagg gttcaattga cacccgataa cacgattacc
      121 ctctacaagt ggattttgaa ggaatttacc atagctgagc gtggcaaggc agtgcggaaa
      181 atagggcgcc acctcgctcc attgattcga cctgctgaca aagtcctgga tctttgctgt
      241 gggacagggc cagcatcttt ttggtttgaa gaacggggcg ctgcggttac cgccatagat
      301 tttgcccctt atatgattgc tctagcacga gatgaggcct caaaacgtaa ctctctggtg
      361 gagtttatcg aaggggacat tttcatatac gattttggac aggatcgtta tgatctggtt
      421 gcttgcttag gaaatagcat ctccgatttt tcattgtctg cttttgtggc attgggaaag
      481 aaagtagcca aggctttgaa gccaggcggg cgatttgtgc tgcaatacca cgatggtttc
      541 ttcccattta ttcataagga gtatgtgcga actggcgttc ttcaggaaac gccagaacgg
      601 gtaactttct tcttcaaaga atacttgccc gaaaaagggg cttatgtgaa aatataccgg
      661 aacgaaacgc ttggagaaga atatcagcgc atcggttaca tatattcggt gccgatggta
      721 cattcagcga tggatggaat cctagacata gagcaacata ttggcttgga tgctgatcaa
      781 tttttggatg tattcacaaa gccagggaaa taaagccatc cattgtcatc agtccaagaa
      841 atctttgtta ggtatgggta aacatctatc cctgccagtc tacccggaat agctaaatta
      901 agaaaattat ctatagaatt caagtaggaa acttattgag agttagaagc aagaagcatc
      961 taaggataaa cctgtagatg aggcaacaaa ggggaaccct gcggtagccg ttgaaattcc
     1021 aagttatatt caagagtaca tagataatcg ggttgagcag tatgcaagct ggtacgatag
     1081 gaagagcgga cctatcaagt ctcgatttct gtatatcaaa gctgtgaccg ttatcggtgg
     1141 cgcacttgtt cctgtattag tcaactccaa ttttgatttt ctgaatgtgg gttttgatgt
     1201 cgccaagatg gctgccacat tattgagctt aattgtggtt gtccttgtgt catgggatag
     1261 tgtttttcag tacggcaagc agtggaaaaa ctatcggtcg acagaacagt tcttgagaca
     1321 agaggtagtt ttatttaagt atcgcaccaa cttttattct ggattggatg atagcaaagc
     1381 gttccaaatt tttatcgaac gcgttgaaaa tgccattgct caagaaaacg cagttacctt
     1441 ggatacgcta acgcgagatt cgaatgttgg caggcagggt gcaagagaag aatgatgtag
     1501 atacttatat ccgtacccct cggcggttcc aggtcggtga ggcagatgat tatccataag
     1561 cgggcgaggc tagcgcctgg gtgagtgtct gggttctaac ggcagtgagg atgatcccgt
     1621 tactgcatgg gtattctcct cacagagttc gggctgtaat ttaacatctc cttttattat
     1681 tcctaattga acaataggag gctgcgatga aggctagaaa ttccacacat gtagagaacc
     1741 gtgtggcagg ggttgccatt caaagcttct caaaagccag acctattctg atgtgcgtca
     1801 tcctgttcat catcgccttg gtctttcgat tgatagattc cattgtgctg cgcttggatg
     1861 aactcctggg ggaacttatc ctcaccaaat cgcttggctt catactggtg cttgtctggg
     1921 tgtggttcac agggcgcaag atcagggata ttggcttgca ctcgcaaaat ttgggtcaga
     1981 gcctgttgat cggcacgctc actacactgg tcgccttcat ctttggatac agtgtagaga
     2041 ttgcatttgc aatacaacaa ggtacgcagc ccactcttca attaggagcc atcgatccga
     2101 aaatgggggt cacgggtggt ttgctcttcg ggctgtggtt gttactgggc aactttgtca
     2161 actcgttcat ggaggaaggt ctgtttcgtg gggtgatggg gcgattggct cggatcaggt
     2221 tcaatttctg gcagaccaac tggttccagg cctttatgtt cggtatctgg caccttccct
     2281 gggtattgaa gtattatctg gtgggagagg tgcagacagg cggcgagatt tttacggcgg
     2341 ttgtctataa ttctcttccc caactcatca ttggtatcgt ttatggatat ttgtacctga
     2401 agaccaatag cttgtgggct gcttggattg cacatacgct gtcgaactcc gcttcgaact
     2461 tcctgcatgt gaatactact gcaggtttag atacaggtct gcccttgcgt atgactgtct
     2521 atttggtggt catgttattc agcttgtttt gggtcaggca ggtgacaaaa aggtatcgaa
     2581 tgcctgaagt aatgccttgg gaatgagtgc aaataaacgg agggtcttat gttaataaga
     2641 attaacgcgc gcacattcac gctcctgttt ttgttatttt ttctggtagg atgcctggca
     2701 cgcacaagcg agcccccgag agtgggtggc tgcgatgatg gccaaacggg cgccccgtgc
     2761 agtgttgtta atgggttcaa tggcaaccaa gtgccttccg atgttatagg agctactatc
     2821 agcggtggtg gagggacttc attccctaat acagtgctcc tggattatgg agttgttggt
     2881 ggcgggaaag gaaatctagc cggcaacctc tctgcagtag ggggtggttt cagcaacgat
     2941 gccagcggca tccgggcgtt tatcgggggt ggggcaaata acacggccag ccgagatagc
     3001 gcggtaattg gcggaggcta tggaaatatc gctgaccaga gctatactac gatcagtggc
     3061 ggagaattaa acaaaactag cggctcatgg gctacgattg gaggaggctc gggtaatatt
     3121 ggcaacgggc gttatgctac ggtaggcggc ggaacccata acttggcgga aagcgcttac
     3181 gcttccattg gtggaggcac ttataatgaa gccggaggtg gcaacagcgc aatcgctggg
     3241 ggtacgcgca ataaagctct gggcatagga tcaaacatcg gtggtggctc gggaaatcgc
     3301 gcggttgggt atcagtccgc gattggcggt ggtctagcta atagcgcaac gggtgattac
     3361 agccttgtgt ctggtggtcg tggcaaccag gcgggaatcg cgaatagcga ccctgaagtt
     3421 gcaagttatg cgacggtagg aggcggctac gaaaacatag ctgctggtgc gttttcggtt
     3481 gtgccaggtg ggcttgcaaa ttacgctctt ggagaataca gcctggcggc tggaaaccgc
     3541 gcccgcgtcg agcccgatca ccctgggaca tttgtatttg ccgattccag tgattatcct
     3601 ttttcgtcct cctcagccaa tgagttcgct gcccgggcta cgggtggggt gcgctttgtt
     3661 accgccgtag atgaaagcgg cgcgcccatg gcaggcgtcc gccttggagc gggcagtggc
     3721 gcttgggagt cgctaagcga ccgtaacgcc aagagttcta ttcaatatgt tgacgtccgg
     3781 caggttctgg aagcgttggt gggcttgccc atcagcacct ggcggtatag agggcaggat
     3841 gagactgtgc gccatattgg cccgatggca caggatttct acgctgtctt tgatctgggc
     3901 caagatgagc gctacatcag cacagtcgat gcagatggtg tagctctagc ttcaatccaa
     3961 ggtctgtatc aactcctgca agggaaggac gcacaaatca tcgctttgca agccgagaat
     4021 ctcagccagg gtcaacaact ggcggatatg gatgcgcgta tcacagcctt ggaaaagcat
     4081 tcttttgaat ctggggcgca aatcaattgg tatctcgcca tgggcgccgc ccttct
//
LOCUS       DBNHOH010000537         3442 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1058009, whole genome shotgun sequence.
ACCESSION   DBNHOH010000537 DBNHOH010000000
VERSION     DBNHOH010000537.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1058009"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..438)
                     /locus_tag="AC16V7_09340"
     CDS             complement(46..438)
                     /locus_tag="AC16V7_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257365.1"
                     /translation="MTCGAITSISEQSLTHTCPFCGSNHVIQKTASQDVLRPRYLIPF
                     QIEAQKCLSIAQEWMGSSWMTPKSLKRVADQALFSGVFLPYWTFDTTATASWRAEVGH
                     TETERYFQDGELKTRTVIRCRWESGKVN"
     gene            904..2046
                     /gene="tal"
                     /locus_tag="AC16V7_09345"
     CDS             904..2046
                     /gene="tal"
                     /locus_tag="AC16V7_09345"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HIR6257366.1"
                     /translation="MTKLHELAALGQAIWLDYIRRAFITSGEMQALIDMGVRGMTSNP
                     TIFEKAILSGQGSKPDQLGEYDSDLERLSKAGKTPLAVYEALAIEDIQAAADLLRSVY
                     DDTEGGDGYVSLEVNPTLASQTMETIEEAHRLWLQVDRPNLMIKIPATVEGLPAITAS
                     IASGINVNVTLIFSLTRYHQVVDAYLKGLEQRLAAGKSINHVASVASFFVSRVDSKVD
                     KHLDSILLENGPHAELAASLKGKAAVANAKLAYSLFQELFGSQRFIALQSQGARVQRP
                     LWASTSTKNPAYSDILYVQELIGPHAVNTVPQNTLEAYLDHGEVRQTISEDNDEARLV
                     LEKLENLGISMEQVTKELEDEGVEAFAKSFTSLLESIESRQNKLRR"
     gene            complement(2086..2838)
                     /locus_tag="AC16V7_09350"
     CDS             complement(2086..2838)
                     /locus_tag="AC16V7_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257367.1"
                     /translation="MTVNFTRAAVALLYFRGYSQVDGILLTWETEVEPDIQGFYIMRS
                     DIENGNYERVSQFIPGLGSDTTGDYYTYIDSNVVGGVAYWYQLEMVDVNNLVTFSEKI
                     RVFAGIYSSTPTVTATALLKSATPTFSQLALTATQGVSATSQVTPSPAETSTSIQETT
                     SGFTEVVTATAVVSATATLIPLPEITLQFPTAGANPESANAGTNTISESTSAERDQQL
                     LVRLGRATILVLIVLVWAVLAGWFYISARRME"
     gene            3028..>3442
                     /locus_tag="AC16V7_09355"
     CDS             3028..>3442
                     /locus_tag="AC16V7_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257368.1"
                     /translation="MKRPPLYTLLRLLLILSPLHLGGAAAPPPPTDTPEAGTISIENA
                     IQQEITNQQDSSLIYLLFETQIGQVSISENGDWAFAQLIPLDAKTGAVVPAEPGLALA
                     QNIEGSWHIFLPNDPQWSGTLNAIPDSLISIEDKEA"
BASE COUNT          956 a          829 c          778 g          879 t
ORIGIN      
        1 gctactcaac ctatttgtcc caggaaccag taaatcatca aatttctaat tcacctttcc
       61 agactcccaa cgacatctga ttacagtgcg cgttttcaac tctccgtctt gaaaatagcg
      121 ttcagtctcc gtatgaccaa cttccgccct ccaggatgca gtagctgtag tgtcaaaagt
      181 ccaataaggc aggaaaactc cgctgaacaa ggcttggtcg gcaacccttt tcagtgattt
      241 gggtgtcatc caactgctgc ccatccattc ctgggcaata gaaaggcatt tttgagcttc
      301 gatttggaag ggtatcaagt aacgcgggcg aagtacgtct tgtgaggctg ttttttgaat
      361 gacatgattc gaaccacaga atggacatgt gtgtgtcaac gactgctcag atatcgatgt
      421 gattgcccca catgtcatgc actccatatc cttgcgagcc tctccccaac catgctccat
      481 gtcttgcatt gtctcaaccg taaattcaag ctcactcgcc tgcgcgccag ttgtctttct
      541 ctcaggcggc gaataatacc cacaatggga gcattttaaa ccaccatctt ccacactata
      601 ggcagttgta gccccacact gagggcaagt aaattcgaca acagcttgtt gggggctcgt
      661 ctgctccgga attggggcgt atacctcaat cccactaacg tttgaaggaa cttcatgaaa
      721 attaggcggc gggaatgaaa catccataaa ttagattata gcaaggattc ctctcagtct
      781 aaactcgatc ggaaaatcga ttatcattag tattcaccgt taaaaggtgg gtatttggcg
      841 atgacaaaat tgccgcaaaa aatgcctaaa tttgaatttc ctcaattagt aattggaggc
      901 ctaatgacca aactacatga acttgcagca cttggacaag cgatctggtt ggattatatc
      961 cgccgagcct tcataacctc gggtgaaatg caggctttga tcgatatggg tgtccgtgga
     1021 atgacgtcca accccaccat atttgaaaaa gctatcttga gtggtcaggg gtcaaagcca
     1081 gatcaattgg gggaatacga ctctgacctg gagaggctta gcaaggctgg taagacccca
     1141 ttggctgtgt atgaggcttt ggcgattgag gatattcagg cagctgctga tttgcttcgg
     1201 tcggtatatg atgatacaga aggtggagac ggctatgtca gccttgaagt aaacccaacc
     1261 ttggcaagcc aaaccatgga gactatcgaa gaggcacaca gattatggtt gcaggtagat
     1321 cgaccgaact tgatgatcaa aatccctgct accgtggaag gattgccagc aatcacagcc
     1381 tcaattgcta gtggcatcaa tgtcaatgta actttaatct tctcccttac tcgttatcac
     1441 caggtggttg atgcttatct aaaaggactt gagcaacgcc tagcagccgg taaatcgata
     1501 aaccatgtcg cctcggttgc ctcgttcttc gtctcccggg tagacagcaa agtggataaa
     1561 cacctcgata gtatcctgtt agaaaacggt cctcacgcgg aattggcggc gtctctaaaa
     1621 ggcaaggctg cagttgctaa tgctaagctg gcatatagcc tatttcagga actatttggg
     1681 agccagcgat ttatcgctct gcaatcacaa ggggcgcgcg tacaacgccc tctctgggcc
     1741 tcaaccagca caaaaaatcc agcttattct gatattctat atgtccagga gttgattggc
     1801 cctcacgcgg ttaacaccgt gccacaaaat actctggaag cataccttga ccatggtgag
     1861 gtccggcaaa caatcagcga agacaatgat gaagcgcgat tggtattgga aaagttagaa
     1921 aatctgggaa tctcgatgga acaggtcact aaggaattag aggacgaagg ggttgaagcc
     1981 ttcgctaaat ctttcacctc gctgcttgag agtattgaaa gccggcaaaa caagttacgg
     2041 agataagttc aaacccttat ttgcggatta gcattccaaa atatatcatt ccatcctacg
     2101 ggccgaaata tagaaccatc cagccagcac tgcccacacc aatacaatca ataccaaaat
     2161 ggttgctcgc ccaagcctaa ccaacaattg ttgatctcgc tctgccgagg tactttctga
     2221 tattgtgttt gtccctgcat tggctgactc tggatttgcg cctgcagtag gaaattgtaa
     2281 tgttatttca ggcaagggga taagtgtcgc tgtagcagat acgaccgcgg tagcagtcac
     2341 aacctcagtg aagcccgatg ttgtttcctg aatactcgtc gatgtttcag ccggcgaggg
     2401 ggtcacctgg gaggttgctg ataccccctg ggtagctgtg agcgcaagct gactaaatgt
     2461 tggcgttgca ctctttaaaa gtgccgttgc cgttacagtc ggtgttgaac tatatatacc
     2521 tgcgaaaacc cgtatctttt cagaaaatgt aactagatta tttacatcga ccatctctaa
     2581 ttgataccag tacgccactc ctcccacaac attagagtca atgtatgtat agtaatcgcc
     2641 cgttgtatca ctgcccaaac cgggaataaa ctggctaacc cgctcgtaat tcccattctc
     2701 gatgtcactg cgcatgatgt aaaaaccctg aatatcaggt tcaacctcgg tttcccaggt
     2761 aagaaggatg ccgtcaacct ggctataacc gcggaaatag agcaacgcta ccgcagcacg
     2821 cgtaaaattg actgtcaaga atgttatcgt cagcaataat atgaggccaa tgagaaagat
     2881 tcgggagcgt tctttcgaac ggagggatga gatgctcata tgaaagagaa gtgtgggaat
     2941 tataccaatg aaacgaaatt atcttgccta acaagtctgt aacggcacac tcttcatgca
     3001 acgatctatg tgaagtatca ttaatgcatg aagcgcccac ctttgtatac gctgctgaga
     3061 ttattactga ttctatcgcc gttgcatctt ggaggtgcag ctgctcctcc accacccact
     3121 gatacccccg aagcgggcac catctctatt gagaatgcaa tacaacaaga aatcaccaac
     3181 cagcaagatt cttctttgat ttatctgctc tttgaaaccc agattggaca agtctcaatc
     3241 tcagaaaatg gcgactgggc tttcgcccag ctaattcccc tcgacgcaaa gacaggtgcc
     3301 gtagtaccgg ctgaacctgg attagcctta gcccagaaca ttgaggggag ttggcatatt
     3361 tttctcccga atgacccaca atggtcaggc accctgaatg ctatacctga ttcattgata
     3421 tccattgaag ataaagaagc ct
//
LOCUS       DBNHOH010000538         2193 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_994312, whole genome shotgun sequence.
ACCESSION   DBNHOH010000538 DBNHOH010000000
VERSION     DBNHOH010000538.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_994312"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(253..1275)
                     /locus_tag="AC16V7_09360"
     CDS             complement(253..1275)
                     /locus_tag="AC16V7_09360"
                     /inference="COORDINATES: protein motif:HMM:NF013811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257369.1"
                     /translation="MHLLFLFLDGIGLGTDNPSINPFARADMPNLQNLLGGKRLLAGA
                     PIETQRATLLPLDACLGVSGLPQSASGQATLLTGVNVPSFLGYHYGPKPNPPLAEFLR
                     NGNIFNTLIKKDKNAALVNAYPPRYFDAVNSGRRLYSAIPLAVTSAGISLKTSDDLHA
                     GNAISADFTAQSWHTHLNLPDTPVISAEEAGARLAKLAVDHDFSMFEFWLSDYAGHHQ
                     DMSAACSLLETFDQVIGGLLDTWDDHSGLILITSDHGNLEDLSTRRHTFNPVPALLIG
                     ARELRSSFVRLAKDSAKRHSRGGSSHGSTPYPDKISLNLTNVAPAILGFLDEHHSQRS
                     DLAISD"
     gene            complement(1282..1860)
                     /locus_tag="AC16V7_09365"
     CDS             complement(1282..1860)
                     /locus_tag="AC16V7_09365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6257370.1"
                     /translation="MRNLLQKLFFNLWYYRKPPWDTGISPPELMEYIANHPPGRALDL
                     GCGTGTNVITLARHGWRVIGVDFARRAIAIARYKARQARIQVDLRVGDVTKFTNPGSF
                     DLVLDIGCFHTLPFALRQEYIHNLTQLLAPQGTYLLYAFFKSEGENGPGLLPADVELL
                     SEHLTLVSRQDGTERGMRPSAWFNFRPNMNAP"
BASE COUNT          546 a          527 c          606 g          514 t
ORIGIN      
        1 ttcggaaatt agaaaaattt atctccatta caattttaac aacccagggc aattgcacat
       61 gccctgaggg gcataccccc caccaaaaca ccccgttttc tctcctcagt ggtgggaaat
      121 cgcccaaatg cgattgccct accgtcattg cgaaggaagc ttgtgggcaa cctgagcaat
      181 cttaaatcgt tatggcggga aggcctctgg gctgacctgc ctgctgccgc caggtaggca
      241 ggcgaagcta tctcaatcgc ttatagcgag atcgcttcgc tgcgagtgat gctcatccag
      301 aaatccgaga atcgccggag ccacgttggt taggttaagc gaaattttgt ctgggtacgg
      361 agtagagcca tgggatgatc cccctcttga gtgacgtttc gccgagtcct tcgccagacg
      421 aacgaacgag cttcgcaact ctcttgcgcc aatcagcagt gcgggtacgg ggttgaaggt
      481 atgcctgcgc gtcgataagt cctccagatt gccgtggtca gatgtgatta agatcaagcc
      541 tgaatggtcg tcccaggtgt ctagaagacc gcctattacc tggtcgaagg tttccaagag
      601 agagcatgca gcgctcatgt cctgatgatg accagcataa tcgctcagcc aaaattcaaa
      661 cattgaaaaa tcgtggtcga ccgctaactt agccagacgt gctccagctt cctctgctga
      721 gatcacaggt gtatcgggta agttcaggtg agtatgccag ctttgggcag tgaagtctgc
      781 agagatggca ttccctgcgt gcaagtcatc actggttttc agggatatac cagcgctggt
      841 cacagccaaa gggatagcag agtagaggcg gcggccgcta tttactgcgt cgaaataacg
      901 gggaggataa gcgttaacca gggcggcatt tttgtccttt ttgatcaagg tattaaatat
      961 attgccatta cgtaaaaatt ccgccagggg tgggttaggt ttgggaccat agtggtagcc
     1021 aagaaatgaa ggaacgttaa cccccgttag cagagtcgct tgaccacttg ctgattgggg
     1081 taaacctgaa actcccaggc aggcgtctag cggaagcagg gtggcgcgct gggtctctat
     1141 cggcgcgccg gctaataagc gctttccgcc caggaggttt tgtaggttgg gcatatccgc
     1201 gcgggcaaat gggttgattg agggattgtc agttcctaaa ccgatgccat ctaaaaaaag
     1261 aaaaagtaag tgcatatcaa ttcaaggggc gttcatattc ggcctaaaat tgaaccatgc
     1321 tgatggtctc ataccgcgct cggtgccatc ctggcgagag accagggtga gatgctcgct
     1381 gagcagttca acgtcagctg ggagcaagcc gggaccgttc tcgccttctg atttgaaaaa
     1441 cgcgtagagc aggtatgtcc cctggggagc cagcaattgg gtcagattat ggatgtattc
     1501 ctgtctgagg gcgaaaggca gggtgtggaa acagccaata tcaagcacga ggtcgaagga
     1561 gcccggattg gtgaattttg tcacgtcgcc cacgcgcaaa tcgacctgaa ttctagcctg
     1621 gcgcgctttg taacgggcga tcgcaatggc gcgccgggca aaatccacgc cgatgacccg
     1681 ccagccgtga cgtgccaggg tgatcacgtt ggtgcctgta ccgcaaccca ggtctagagc
     1741 acgaccaggg ggatggttgg cgatatattc cataagctcc ggcggtgaaa ttccagtatc
     1801 ccaaggcggt ttacggtagt accacaaatt gaagaacaac ttctgcagta gattgcgcac
     1861 aaagtgattt taaccgataa cttctatctg aaaagcagag ttcctttcga caattaaaaa
     1921 tctcctttgt ttcgagaaag caaggatggg agtgaatatt gtgctccgcc ttcttttttt
     1981 gtccggtttg tttgacaagc cagccgatat accttagaat caatcgctgt ctgaaaataa
     2041 ctcacaggaa gggttccctt atgaaagagt atacaaccga gttcatccgc aatatcgcgc
     2101 tggcttccca tagcggctct ggcaagacta tgctggtgga agcaatggcg cattttaccg
     2161 gtgcgaccac tcgcctaggg aaggttgagg acg
//
LOCUS       DBNHOH010000539         4483 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1762873, whole genome shotgun sequence.
ACCESSION   DBNHOH010000539 DBNHOH010000000
VERSION     DBNHOH010000539.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1762873"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2043
                     /locus_tag="AC16V7_09370"
     CDS             <1..2043
                     /locus_tag="AC16V7_09370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR6257371.1"
                     /translation="DSQVLLSFLVGAGLATLGIFFTRVQKETSPGKNLAGMASRIDLV
                     VALALWVFAALHWLSIPTQTSWFVAGPRYPNYQYYPNSDAYLYDSTAQSLLVGSGFEM
                     GNREYARRPVYIMFLAGLYRLIGQDYETVTLVQSAIFAVFPVLIYWLTLTLHNRLSAL
                     IAALLVLFREGNAIALAERITVSNSRMFMAETPATIGVVLFACLVVWWLRQSGRRPVI
                     ALILGGVLGVFMQIRIEVAFLLPSFLLLAGFVTCRAPKQWLKNAVLISLGMCLFLAPW
                     VWRNWHRTGLVFLERPAERLAFVAVRSQTGVGDLQTASAPAQDTLAAKINEASVFLRR
                     MSSHYINSQAQAFLIFPDAFRLMDSTIGYLEQGKSPRFWKACCSRQDYIDRFTFWMWG
                     KWEGAIPAQSVASIIINMLLVVIGLVKLWQWKRPAGIFLFIVSIGYYLATSAVRVSGG
                     RFVQVVDWIWIVCFSVGLAQIIEWTVASLSSANLPTWLFGEVHQAQIGWQGTPIQAIT
                     PLRTYMGVSLAIILAGASLPLVEAIIQPRYTEAARQAWLAELQQSELLEQNYPDVLET
                     LKNIRRYRLIVLHGRALYPRYYASGEGESSTKITPFTPRDFARFSFYLVGPRNIGVLL
                     PLDEQPLVHFPNGEDVLVVGCMENGYLRALVVYLHSQDAVLISRPLPASMACQAGP"
     gene            complement(2211..3140)
                     /locus_tag="AC16V7_09375"
     CDS             complement(2211..3140)
                     /locus_tag="AC16V7_09375"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR6257372.1"
                     /translation="MVSPKPPESGFPPVIDYEGSDYQSTFWEKGERDYEDQVEAVALR
                     RLLPSSGKLLLEVGAGAGRNTPRYGGFERVVLLDYSLSQLRLAQDRLGHGERYIFVAA
                     DAYRLPFAPSQFDAATMIRTLHHMVEPQRALGQVGEALQPGGIFILEYANKHNLKAIL
                     RYSLRRQEWSPFSLEPVEFAALNFDFHPQAVQDWLYACGFTIERQLTVSHFRVGLLKR
                     WVPLRLLVALDSLAQWTGNWWQLSPSAFVRARSVRRDAIQHYNTLTFRCPACKYYPLE
                     DISGTLNCTNCARSWPIREGIYDFREKYLADPE"
     gene            complement(3299..>4483)
                     /locus_tag="AC16V7_09380"
     CDS             complement(3299..>4483)
                     /locus_tag="AC16V7_09380"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose dehydrogenase family protein"
                     /protein_id="HIR6257373.1"
                     /translation="ERNVRSGRLTFTTSYAEGLEGSEFVFIAVGTPSGVDGEADLRYV
                     AASARSIAENMTAPLIVINKSTVPVGTGDWVADIIRSNQRTPIPFSVVSCPEFLREGS
                     AISDFMQPHRTVLGSLDKEAAEKVAQLHLTLRAPIVITDLRTAEMIKYASNAFLATKI
                     SFINEIANICEALGADVKEVAAGMGYDKRIGSMFLDAGLGYGGSCFPKDVKALAYMAA
                     QMGRHPQLLNAVMEINDDRRPMIVNRLHEMLGDLSGKTIGLLGLSFKPNTDDMRDAPS
                     ITVAELMRSEGAQVRAYDPVAMDIARPLLPFVKMYEDPYSMAEGCDALMVVTEWNEFK
                     QLDLERVRDLMHSPNILDGRNIYDPNLLAKLGFQYRGVGRGYNGSKLLDANRTEFVKA
                     TA"
BASE COUNT          953 a         1200 c         1225 g         1105 t
ORIGIN      
        1 gatagccaag tcctgctctc attcctggtt ggggcaggct tagccaccct cggcatattt
       61 tttactcgag ttcagaagga aacttcacct ggaaaaaatt tggctggtat ggcatccagg
      121 atcgacctgg ttgttgctct tgccttatgg gtctttgcag cactacactg gctctctatc
      181 cccacccaga caagctggtt tgttgcagga ccgcgttatc ccaactacca atactaccca
      241 aactccgacg cgtatttata tgattccact gcacaaagct tgctggttgg ttcgggcttt
      301 gagatgggga atagggagta tgcgcgccgg ccggtataca taatgttctt agccggctta
      361 tatcgactga taggtcagga ttatgagacg gttacgctgg tgcaatcagc aatttttgcc
      421 gtctttcctg tgttgattta ttggcttacc ctgacgctgc acaatcgcct ttccgcactg
      481 atcgcggcgt tattggtgtt attccgagaa gggaatgcca tagcgttggc ggaaaggatt
      541 acggtctcga actccaggat gttcatggca gagacgcccg ccactatcgg cgtggttctg
      601 tttgcctgtc tggtcgtctg gtggctgcgt caatctggtc gcaggccagt catcgcgctc
      661 atcttgggtg gcgtgttggg tgtatttatg cagatccgca tcgaggtcgc ttttttgttg
      721 ccctcctttc tcctgctggc aggatttgtc acctgtcgtg ctccaaagca gtggcttaag
      781 aacgccgttc tgatttcgct agggatgtgt ttgttcttag caccctgggt gtggcgcaac
      841 tggcaccgta ctgggctggt attcctggag agacccgctg aaaggctggc ctttgttgcg
      901 gtgcgatctc agactggcgt gggcgacctt caaacagcat ctgcccctgc ccaagatact
      961 ctggctgcca agatcaacga agcctccgtc ttcttacggc gcatgagttc gcattacatc
     1021 aatagccaag ctcaggcgtt tctgatcttt ccagatgctt ttcgcctgat ggacagcacg
     1081 ataggatacc tggaacaagg aaaatccccc cgtttttgga aggcctgttg ttctcgacaa
     1141 gactatatcg accgcttcac tttctggatg tggggcaaat gggagggagc tattccggcc
     1201 caatccgttg catcgatcat aatcaacatg ctgctggttg tcattgggct tgtcaaacta
     1261 tggcaatgga aacgccccgc ggggatattt ctgttcatag tgagcatcgg atattatctg
     1321 gccacaagcg ctgtacgtgt ttctggtggt cgtttcgtcc aggtcgtcga ttggatatgg
     1381 atcgtttgct tcagcgttgg gttggcacaa attattgaat ggacggttgc ctctttgagc
     1441 agcgcaaatc tgccaacctg gttatttggt gaagtgcatc aagcacagat cgggtggcag
     1501 ggaacgccca tccaggctat aacaccgctt cgaacctaca tgggggtcag cctggcgatc
     1561 atcttggctg gcgcttccct tccattggtt gaggccatca tccagccgcg ttacacagag
     1621 gccgcgcggc aagcctggct cgctgagttg cagcaatcgg aattgctgga gcagaattac
     1681 ccggatgtgc tggaaaccct gaaaaatatc cggcgatatc gtctgatcgt cctgcatggt
     1741 agagcgctct atccccgcta ttatgcaagc ggagaaggcg agtctagcac taaaataaca
     1801 ccctttacac ccagagattt tgcgcgcttt agcttttacc tggtcggtcc tagaaacatt
     1861 ggcgtactgc ttccattgga tgaacaaccg ctggtgcatt ttcccaacgg agaagatgtt
     1921 ttggtcgttg gttgcatgga aaacggctac ttgcgggctt tggtcgtcta ccttcattca
     1981 caggatgctg tcctgatcag ccggccattg cctgcctcga tggcttgcca agcgggacca
     2041 tgaccgagat acatcgttag gctagctttt gtcctcatgt ttgcaggtta gcatgagaaa
     2101 gacccctcta cagccctgtt tgtcggcact gctcgaagtc gctatcatgt tctagtccac
     2161 catatctgcg tacatatggc tgtggccgaa cgtcaagatc atagacgctt ttactctgga
     2221 tctgccaagt atttctccct aaaatcgtat atcccctctc gtatgggcca gctcctggcg
     2281 caattcgtgc agttcaaagt tccagatata tcttctaatg ggtagtactt gcatgccggg
     2341 caacggaaag tcagagtgtt gtagtgctgg attgcatctc gccgtactga tctggcgcgg
     2401 acgaacgcgc tgggcgagag ttgccaccag ttgcccgtcc actgggcaag cgaatccagc
     2461 gccaccagca ggcgcagtgg cacccagcgt ttgagcagcc cgacccggaa gtgcgaaaca
     2521 gtcaactggc gctcgatagt aaagccacat gcatacaacc aatcctgcac tgcctgcggg
     2581 tggaaatcaa agttaagtgc cgcgaattcc acaggttcaa gcgagaaagg gctccattcc
     2641 tggcggcgca gggagtagcg caggatggct tttaggttgt gtttgttggc atattccagg
     2701 ataaagattc ccccgggctg gagcgcctcg ccaacctgcc ctagggcgcg ctgtggctcg
     2761 accatatggt gcaaggtgcg gatcatggtg gctgcatcga actggcttgg tgcaaagggt
     2821 aggcggtaag cgtctgcagc tacgaagata tagcgctcgc catgacccag gcgatcctgt
     2881 gccaagcgca gttgagagag cgagtaatct agcaagacga cgcgctcaaa tccgccgtag
     2941 cgcggtgtgt ttcgacctgc gccggcccca acttccagca gcaacttccc ggaagagggc
     3001 agcaaacgac gcagtgccac cgcttcgacc tggtcttcgt agtcgcgctc gcccttctcc
     3061 cagaatgtag actgataatc agagccttcg tagtcaataa cgggcggaaa cccgctctca
     3121 ggaggctttg gactcaccat atgaatctct gatcccccct ggtcttgctt cctattggga
     3181 aggggggatc aaatctaatt aataagcgca taccccaaat tccgtatcta tttcccttcc
     3241 ccggtgtagg gaggggagtg gggaaatggc agaaaacaaa cttgccgtta aaaaactttt
     3301 aggctgtcgc cttgacaaac tccgtgcggt ttgcgtccag gagtttggag ccgttgtagc
     3361 cgcgccccac accgcgatat tggaagccca gcttggcgag caggttgggg tcgtagatat
     3421 tacgcccatc aagaatgttt gggctgtgca tcagatcgcg caccctctcc agatcgagct
     3481 gcttgaattc attccactcg gtgacgacca tcagggcatc gcaaccttcc gccatactgt
     3541 aagggtcttc atacatcttg acaaacggca ggagcgggcg ggcaatatcc atagccaccg
     3601 ggtcgtaggc gcgtacctga gccccttcgc ttctcattaa ttctgccacg gtgatggacg
     3661 gcgcatcgcg catatcgtcg gtgttgggtt tgaaagacag tcctagcagg ccaatggtct
     3721 tgccacttag atctccaagc atctcatgca gacgatttac aatcatggga cggcggtcgt
     3781 cattgatctc catcacagcg ttcaacaact gcgggtggcg acccatttgc gccgccatgt
     3841 aagccagcgc cttgacatcc ttgggaaaac atgagccccc gtagcccagg ccggcatcca
     3901 ggaacatcga cccgatacgc ttgtcatagc ccatgcctgc agctacttct ttgacatcgg
     3961 cgcctagcgc ctcgcagatg ttggcgatct cgttgataaa tgagattttg gtcgccagga
     4021 atgcattgga tgcgtactta atcatctcgg cagtgcgcag gtcggtaatg acgatgggag
     4081 cgcgcagcgt caggtgcagc tgggccactt tctcagccgc ctctttgtcc agcgagccga
     4141 gcaccgtgcg gtgcggctgc atgaaatcgc tgatagccga cccttctcgc aagaactctg
     4201 ggcaagaaac gacagagaat gggattggag tacgctggtt gctacggatg atgtcggcaa
     4261 cccagtcgcc ggtgcccact ggaacagtcg atttgttaat gacgatcagt ggggctgtca
     4321 tgttctcagc aatggagcga gccgaggctg caacatatcg caggtcggcc tcgccgtcca
     4381 cgccagatgg ggtccctact gcgatgaaga cgaactcgct accttccaac ccctctgcat
     4441 aggaagttgt aaaggtcaag cggccgctgc gtacgttccg ctc
//
LOCUS       DBNHOH010000540         1939 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1762964, whole genome shotgun sequence.
ACCESSION   DBNHOH010000540 DBNHOH010000000
VERSION     DBNHOH010000540.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1762964"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..1060
                     /locus_tag="AC16V7_09385"
     CDS             29..1060
                     /locus_tag="AC16V7_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HIR6257374.1"
                     /translation="MNAYLGVDVGSVTTKLVVLDNDYNLLSSLYLRTRGKPIDVVQQG
                     LCEIAERLEGMSIEINGVGTTGSGRYLAAVVIGADIIKNEITAHAVATSYYVPGVQTI
                     VEIGGQDSKIIILRDSVAVDFGMNTVCAAGTGAFLDQQAARLNIKVEDIGLLALQSKT
                     PVRIAGRCTVFAESDMIHKQQMGHRVEDILYGLCQAMSRNYLNNVGLGKEVREQVAFQ
                     GGVAFNQGMIRAFEEALDVSIHVPPHHEVMGAIGAAMLAREAMLYSTQTTRFIGFSAS
                     QMDYRTTSFECRFCSNRCEIVQVSVNKHVVARWGGRCDRWDVNDAIINRPTASIAVAM
                     PSKESCEVC"
     gene            1449..>1939
                     /locus_tag="AC16V7_09390"
     CDS             1449..>1939
                     /locus_tag="AC16V7_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6257375.1"
                     /translation="MSPKLNFTHRRKNLVMLTAVIILILATALILFRPVDAQNFISRP
                     NPVQNYNQAEQRIQALQSEETDQLNPVCRTQFLSYGKKVERVIVFIHGNTTCPQQFAE
                     LGKLFYQNGYNVLIAPLPRHGLADRMTEAQAQLTAEHLATYGDKVVDIAQGLGDHVTL
                     AGLS"
BASE COUNT          470 a          474 c          514 g          481 t
ORIGIN      
        1 gccagcatga cttttcaagg agaacgctat gaacgcatat ttaggtgtag atgtaggctc
       61 agtgacaacc aagttggttg ttcttgacaa tgattacaac ttattgtcat cactctatct
      121 gcgcacgcgc ggtaaaccca tcgatgtcgt tcaacagggt ctttgtgaaa ttgcagaacg
      181 cctagagggt atgtccattg agatcaacgg cgtaggtact accggcagcg ggcgatatct
      241 ggctgctgta gtgatcggcg cagatattat caagaatgaa atcaccgctc atgcagtggc
      301 aacgtcttat tatgttcctg gcgtacaaac gatcgttgag attggcggcc aagattccaa
      361 gattatcatc ttgagggata gcgttgcagt cgattttgga atgaacacgg tgtgcgctgc
      421 aggtaccggc gcattcctgg atcaacaggc tgcacgcctg aacatcaaag tcgaagatat
      481 agggctgcta gccttgcaat ccaaaacgcc ggtgcgtatt gcgggacgtt gtaccgtatt
      541 tgcagaatcc gacatgatcc acaagcagca gatggggcat cgcgtcgagg atatcctgta
      601 tggcctttgc caggccatga gccgtaacta tttgaacaac gttgggctgg ggaaagaagt
      661 tcgtgaacag gtcgcattcc aaggtggcgt ggcctttaac caggggatga tacgtgcttt
      721 cgaggaagct ttggatgttt ccattcacgt tcctccgcat cacgaagtga tgggagcaat
      781 tggcgcggca atgctggcgc gtgaagcgat gctatattca acccaaacca cccgcttcat
      841 tgggtttagt gccagtcaaa tggattaccg caccacctcc tttgagtgcc ggttctgttc
      901 gaaccggtgt gagatcgtgc aggtgtccgt aaacaaacat gttgttgcac gctggggagg
      961 ccgttgtgat cgctgggatg tgaacgatgc cattatcaac cgcccgactg catcgatagc
     1021 tgtggccatg ccatcaaaag aatcttgcga ggtttgttaa tgctgttgat aaactgagcc
     1081 tcagcttaac tcttgggtga cataccaatc attattccac tctccgcctc gcttgggcga
     1141 gccgcaactt gacatgtggg agataactta tgcagagtga taatactcca gcgaaaaatt
     1201 cccgagcctt gaaatagctc ctggtattga tcgcgggaat gacgatgctc acagcgtttc
     1261 gtgttgcgtg ggtgattgtg ggcgcgctaa attcgtcgct gctgggagtt tttttccacg
     1321 gagacggcag atctctgcca gcccattgcc gctaatgcta accaccgggc agtattctag
     1381 gtgtgcatta cacattgcta atacctgctt tgccaattaa acagagttac aatcctgggg
     1441 aggtcggagt gagtccgaaa ttgaacttca cgcaccggag gaagaatttg gtcatgttga
     1501 ccgcggttat catccttatt ctagcgaccg cgcttatctt gttccgacct gtagatgcac
     1561 aaaatttcat ttcgcgcccc aatcctgttc aaaattacaa ccaagctgag cagagaatcc
     1621 aggccctcca gtcagaggag acagaccagc tcaacccggt ctgccggaca cagttcctgt
     1681 cgtacggcaa aaaggtcgag cgggtcatcg tttttatcca tggcaacaca acctgcccac
     1741 agcagtttgc cgagttgggt aagctgttct accaaaatgg atataacgtc cttattgcgc
     1801 ccctgcccag gcatggcctt gccgatcgca tgaccgaggc gcaagctcaa ctgactgccg
     1861 agcatttagc tacctacggg gataaagtgg tagacatcgc acaggggcta ggggatcacg
     1921 taaccctggc aggcttgtc
//
LOCUS       DBNHOH010000541         7074 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1314096, whole genome shotgun sequence.
ACCESSION   DBNHOH010000541 DBNHOH010000000
VERSION     DBNHOH010000541.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7074)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7074)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7074
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1314096"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..1143
                     /locus_tag="AC16V7_09395"
     CDS             106..1143
                     /locus_tag="AC16V7_09395"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR6257376.1"
                     /translation="MRPPRFPIILIPAFTLLVTLSCALGNRLQITYQTPATASVPPEF
                     FTPTPGEPLSTEEAEAPSSEAPSESEPPSVSEEFAPCEKKVCIESGSFLLKRPIGPDG
                     RFTIDTSSRFGTLRRRLRDAYLGVQFLNSTGTPVLAAWDGDVVVAGDDSRISYGVRPN
                     MYGNLVILRHFLPGVPEPVYTLYAHLSEVSVKAEGDVEAGEQIGLVGMTGSIFGSTLY
                     FEVRYGENSPEAVRNPELWLELLPDETGDSMGALAGQVVDRQGNYVSLSNILVENLRS
                     ASQGLDRQIYLKTYIEKDLMGQSPWGETFAVSNLPEGTYQISFWYGAELYQRQVEVQP
                     GMLTFVPFEVK"
     gene            complement(1148..2554)
                     /locus_tag="AC16V7_09400"
     CDS             complement(1148..2554)
                     /locus_tag="AC16V7_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6257377.1"
                     /translation="MKKLLPLLVLILFLLPSRVLADIAPPDQPAGANPIPGSEATQVR
                     MLSERVLIDVQAKAPQGSLGQAKVTASFSMRNLGSAVEMLPVRFPLSYPGGVAGGIQG
                     NLEIRDFAARVGGKAVTTRRIIGADSQPWAEFDVTFPVESDIQVEVAYLVTGVGEYPF
                     IAFYYILETGAGWQGTIGSADLVLRLPYEANSLNVIFDEELGWSKTTPGGLLSGQEVR
                     WHLEDFEPSKGDNLQVSLVMPAAWNAVLTERANVARNPDDGEAWGRLGKAYKEILFLR
                     KGVRQDAGGQELYRLSQEAYENALERLPKDALWHAGYADLLYQAYYWKEFFTAQPDHS
                     AMLRALEQLRVSIELDPQNTKAIELLEDMKYALPEAVRQEGDTYLLLWLTATPTTRPT
                     FTNTPSQTPLPPTATGTPMPTPALERSPTETPTHTPQLPTTESAATPEPPSGSPVCGT
                     AALLPLALIAWVWKRRER"
     gene            complement(2556..4799)
                     /locus_tag="AC16V7_09405"
     CDS             complement(2556..4799)
                     /locus_tag="AC16V7_09405"
                     /inference="COORDINATES: protein motif:HMM:NF013109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902358 - sulfate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR6257378.1"
                     /translation="MASPESKLTLQARLTTWMADELRPGQLVRSLTTSLLVYILEIIV
                     AISFAALIFSGELSSQLPFGIGLILIGDMLLVLCVSLFSSYSGSIAVEQDAPGVILAL
                     IAATVVAALPAGANQTEKFSTVVMMIVITSVGTGLFFMVLGLFKLGGLVRYLPYSVMG
                     GFLAGTGWLLAIGGVSLMADASSLSLTLFQPDLLIRWLPGVLLALVMLLAVNRSGNPL
                     VLPVIFALGIGFFYAIAWMTRTSFAELNTQGWLLGSFPSGGLWQFPLQSELLSNVNWP
                     ILSNQIINLSPVLIVSVIALLLNANGLELIIKKDVNLNRELITAGIGNLAAGLGGGIV
                     GYHAISLSSLNHTASGGKRLVGLFTALLVGITVFLGAGYLSYAPKMVLGALLVFLGLS
                     FLYDWVYQAWFKFPKSEFVIILTILLVIAFRGFLEGIALGLVMAIILFVVNYSQVKVV
                     KQTLYGTTYRSRVTRSRSQWEILTARGDELCIFKLQGFIFFGTAISLFEQVRDLTRRT
                     DSRPMRFAVLDFAQVTGLDSTALLSFNKLLDLAQEKSFTLVLTGLTGRASEQFEKGGF
                     QAQMEALQLFADLDHGIEWCENQILADAPVDIEAESSLQAQLAAILPGEQTRIERLIG
                     YLERKEIAPGEYLIRQGDAPDMMYFIESGQVTARLEAPGQKPVRLETMGGGRTIGELG
                     FFLGVKRTAAVVADKPSVVYGISKDMLRQMEEIHPEEANTFHRIIIQLLGERTVHLIR
                     SVDALQR"
     gene            complement(4879..6018)
                     /gene="nagA"
                     /locus_tag="AC16V7_09410"
     CDS             complement(4879..6018)
                     /gene="nagA"
                     /locus_tag="AC16V7_09410"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HIR6257379.1"
                     /translation="MRNSELTLTGLALLPDGVISKATIHISNGTITQVSRGADPQADI
                     QTTGTILPGFIDLQLNGAFGYDFTLNGASVAAVSERLPSTGVTSFIPTIITSPFKDYP
                     VRLSEIAGAMQIAKGAQPLGVHLEGPYLNPAKRGAHDAAYMRPIDLDEILGWADPALV
                     RIVTLAPELPGALEAIRALRNNGITVSLGHSNATLEQANAGFEAGANWATHLYNAMSP
                     PHHREPGLTGAILTSSVPCGLIPDGVHVHPSMVKLAFHAKGVNGITLVTDAMEALGMP
                     SGIYRLGDREVHVSEDRVQLADGTLAGSKIEMDAAVRNMMAFSGCTLAQAASMASLIP
                     AQVLGLDRKGAIGPGYDADLVILDQTLHVSLTIVAGKLSFERSPV"
     gene            6199..>7074
                     /locus_tag="AC16V7_09415"
     CDS             6199..>7074
                     /locus_tag="AC16V7_09415"
                     /inference="COORDINATES: protein motif:HMM:NF015948.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HIR6257380.1"
                     /translation="MEKVFLFKAEGYDPRWIEAGIKQAAQALLVSLPAQGDVFLHVDC
                     PWADSRFAPHAHTHPAMIEGVAKALSGASLSLGGCSLPYFPTRYSFKQAGYDALARKL
                     DAELVAFEEVATRQVELGGTAVVNKQVGLPSAWDQAAFRISLPKLRCSTLVSFAGALR
                     HMQSLLSQAAQVTDSHRLPEKMVDLLPTVSPNLIVVDAIHALHKGGELSGEPVELGLI
                     IIGTHPLAVDVVCAVLLGLEPPRVDFLGEAASRGLGPSSLDEIELLGDLTLDEVRSLS
                     QKVQMVDPDPVHYVLP"
BASE COUNT         1565 a         1943 c         1958 g         1608 t
ORIGIN      
        1 cggcagttca aggccgtttc aatcgcagtt tcactgtggt tgggcaacaa tcctaacatt
       61 tagcaccaga aaccaggccc ttataggatc ggaggtatcc aggcgatgag accccccaga
      121 ttcccaatta ttctcatacc ggcgtttact ttgctggtca cactcagttg tgccctgggt
      181 aaccgtctgc aaataaccta tcaaacgcca gcgacagcca gcgtccctcc ggagtttttt
      241 acaccaacgc caggcgaacc cctttccacg gaagaggctg aagctccttc atcagaggcg
      301 ccatcggaaa gcgaacctcc gtccgtttca gaagaattcg ctccctgcga gaagaaggtg
      361 tgcatcgaga gcggctcatt ccttctgaag cggcctattg gtccagatgg gcgatttacc
      421 atcgacacct catctcgatt cggcaccttg cgcaggcgtc tgcgcgacgc ctacctcggg
      481 gtgcaattcc tcaattcaac cggcaccccg gtcctggcag cctgggatgg ggatgtggtc
      541 gtggcagggg atgacagccg gatcagctat ggcgtgcgcc ccaatatgta tggcaacctg
      601 gtcatcttgc ggcatttctt gcccggagtt cccgagcctg tctacacctt gtacgctcac
      661 ctgtccgagg tctcggtcaa ggccgagggt gatgtagaag ccggagagca gatcgggctg
      721 gtaggtatga ctggctctat attcggcagc accctgtatt ttgaggtgcg ctatggagag
      781 aactctcctg aggcggtacg caacccggag ctttggctgg agctgctccc cgatgaaaca
      841 ggcgactcga tgggcgcgct cgccgggcag gtggtggatc gtcaagggaa ctatgtgtcg
      901 ctaagcaata tcctggtcga aaatttgcga tccgccagcc agggtctgga ccgtcagatc
      961 tatttgaaga cctacatcga aaaagacctg atgggacaaa gcccctgggg cgagactttc
     1021 gccgtcagca atctacccga gggcacctat caaatttcat tctggtatgg cgcggagctc
     1081 taccagcgcc aggtggaagt gcaacccggc atgctgacgt ttgtgccgtt tgaagtgaag
     1141 taaagcatta gcgctcccgt cgcttccaga cccaggcgat caaagccagc ggtagcagcg
     1201 cggctgtacc gcaaactggt gaccctgagg gcggctcagg tgtagcagcg ctttccgtag
     1261 tcggtagttg cggcgtgtgg gtgggggttt cggtcggcga tcgctccaag gcgggggtgg
     1321 gcatgggcgt gcccgtcgcg gtgggcggca ggggtgtttg gctgggcgta ttcgtaaagg
     1381 tgggccgcgt ggtgggtgtg gcggtcaacc acagcagcag gtaagtatcc ccctcctgcc
     1441 tgacagcctc tggcagggcg tacttcatat cctccagaag ctctatggct tttgtgttct
     1501 gcgggtccag ctcgatggac acgcggagct gctccaatgc gcgcagcata gccgagtgat
     1561 ccggttgtgc ggtgaagaac tccttccagt agtaggcctg gtacagcaga tcggcatatc
     1621 ctgcatgcca taaagcgtct ttcggcaagc gttccagcgc attttcatag gcttcctggc
     1681 tcagccgata aagctcttgc ccaccggcat cctggcgcac acccttgcgc aggaaaagga
     1741 tctctttata ggccttgccc agtctccccc aggcctcgcc atcatccgga ttgcgggcga
     1801 cgttagctct ctcggttaaa actgcgttcc acgctgcagg catgaccagg gagacctgca
     1861 ggttgtcgcc cttggagggc tcaaagtcct ccaaatgcca gcgcacttcc tgtccgctta
     1921 gcagcccgcc gggtgtggtc ttcgaccagc ccagctcttc gtcaaaaatc acgttcaagc
     1981 tgttggcttc gtatggcagg cgcaagacca ggtcggcgct gccgatggtg ccttgccatc
     2041 cagctccggt ttccaagatg taataaaagg cgatgaaggg atattcgccc acaccagtga
     2101 ccagataggc gacctccacc tggatatcgc tttccacggg gaaggtcaca tcgaactcgg
     2161 cccagggctg ggagtctgct ccaatgatgc ggcgggtcgt gactgctttg ccgcccacac
     2221 gcgctgcaaa atcccgaatc tccaggttgc cctggatgcc gcccgcaaca cctcccgggt
     2281 aagagagcgg gaaccgcacg ggtagcatct ctactgcgct ccccaggttt cgcatgctga
     2341 agctggcggt aaccttggcc tggcccaggg agccctgcgg agctttggct tgcacatcga
     2401 tcagcacacg ttcagacaac atgcgcacct gtgtggcctc gctcccgggg attggattgg
     2461 cgccggctgg ctggtccggt ggggcgatat cagccagaac ccgactggga agaagaaata
     2521 ggatcagtac aagtagaggc agcagttttt tcatgtcagc gctgcaaggc gtcaaccgac
     2581 ctgatcaggt ggacagttct ttcgcccaaa agctggatga tgatgcggtg aaaggtgttg
     2641 gcctcttcag ggtggatctc ctccatttgc ctcaacatgt ctttcgagat gccgtacacc
     2701 acactgggtt tatcggcgac aaccgcagct gtgcgtttga cccccaaaaa gaatcccagt
     2761 tctccgatgg tgcggccgcc ccccatcgtc tctaatcgca ccggtttttg cccgggggcc
     2821 tccagccgcg cggtgacttg accagattcg ataaaataca tcatatctgg agcgtctccc
     2881 tggcggatca gatattctcc tggggcaatt tccttgcgct ccaggtaccc tatcaatctt
     2941 tcgattctgg tctgctcacc gggcaagatg gctgccagct gtgcctgtag gctgctttct
     3001 gcttcaatat caacaggtgc gtcagcaagt atctggtttt cacaccattc gatcccatga
     3061 tcgagatccg cgaagagctg caaggcttcc atttgggcct ggaagccgcc tttctcgaac
     3121 tgctcgctgg ccctgccggt cagcccggtt agcaccaggg taaagctttt ttcttgagcc
     3181 aagtccagca gcttgttaaa gctgagcagg gcagttgagt ccagaccggt gacctgtgcg
     3241 aaatccagca cggcaaagcg cattggcctt gaatctgtgc gcctcgtcag atcgcggacc
     3301 tgttcgaaca ggcttatagc ggttccgaag aagatgaatc cctggagttt aaagatgcat
     3361 agttcatccc ctcgggctgt taggatttcc cactggctgc gactgcgggt cacccggctg
     3421 cggtaggttg tgccatataa tgtttgtttg accactttca cctggctgta gttgacgaca
     3481 aacaggatga tggccatcac cagcccgagt gcgatcccct ccagaaatcc cctgaatgcg
     3541 ataaccagca agatggtcag gataatcacg aattcgcttt tagggaactt gaaccaggct
     3601 tggtataccc aatcgtacaa gaatgagagc cctaagaaga caaggagtgc gcccaggacc
     3661 attttagggg catagctaag atagcccgcg cccaggaata ccgttatacc tactagcagc
     3721 gcagtaaaca ggcctaccag acgtttgcca ccactcgcag tgtggttcag gctggataag
     3781 ctgatcgcat ggtagccgac gatcccgcca cccagaccag cagccagatt ccctatcccc
     3841 gcagtaataa gctcgcgatt caaattaaca tcctttttga tgatcaattc gagaccgttg
     3901 gcatttagta gcagggcgat gacgctgacg atcaacaccg gcgataggtt gataatttgg
     3961 ttggagagaa ttggccagtt cacattggat aatagttccg attgcaaagg gaattgccac
     4021 aagcccccag atggaaatga ccccaacagc cagccttgtg tgttaagctc tgcaaatgat
     4081 gtcctcgtca tccaggctat ggcataaaaa aagccaattc caagtgcgaa tatcacaggc
     4141 aataccaggg ggttgccgga acgattgacg gccaagagca tcaccagagc aagcaacacc
     4201 ccagggagcc agcggatgag cagatcgggc tgaaataagg taaggctcaa cgatgaggca
     4261 tctgccatta agctcacacc acctatggct agcaaccagc ctgtgccagc caggaagccg
     4321 cccatcaccg agtaaggcag gtagcgcact agcccgccga gcttaaacaa ccccaacacc
     4381 ataaaaaaca agccggtccc cacgctggtt attacgatca tcatcacaac ggtcgagaat
     4441 ttctcagttt ggttggctcc tgcgggcagc gcagctacca cagtagcggc aatcaaagcc
     4501 aatatcactc cgggtgcgtc ctgttcaacg gcaatcgagc ctgaatacga gctgaataag
     4561 gagacacaaa ggaccaaaag catatctccg atcagaatca agccaatacc aaaagggagt
     4621 tggctggaca gctctcccga gaaaatcagt gcagcgaaag agatagccac gataatttct
     4681 agaatataaa ccaggaggct ggtggtcagg cttctaacca attgccctgg gcgaagttca
     4741 tccgccatcc aggtagttag acgcgcttgc agggtcagct ttgattcagg tgatgccatc
     4801 tttccacctc taatctcgat tgaaagcgat atgtaacatg taaagaggtc gcttaactgt
     4861 aaggaactct taatccaatt aaacgggcga cctttcgaaa gacagctttc ctgccacaat
     4921 ggttaaggaa acatgcagtg tctggtctag tatgaccaga tcggcgtcat agccaggccc
     4981 aattgcgcct ttacgatcta atcccagcac ctgcgctggt ataagggacg ccatgctagc
     5041 tgcctgcgcc agtgtgcatc cagagaaagc catcatgttg cgcaccgcgg cgtccatctc
     5101 tattttgctt ccagccagtg tgccgtcggc taactgcacg cgatcctcac tgacatggac
     5161 ctctcgatct cccaggcgat agatgccaga aggcatgccc aaggcttcca tggcatccgt
     5221 gaccagcgtt atgccattta cccctttggc gtggaatgcc agcttgacca tgctcgggtg
     5281 cacgtgcacg ccatccggaa tcagcccaca tggcactgat gaagtcagta ttgctccggt
     5341 taatcctggc tctcgatgat gaggggggct catggcattg tacaagtgcg tggcccaatt
     5401 cgctcccgcc tcgaacccgg cgttggcttg ctcaagagtg gcattggaat gtccaaggct
     5461 aacagtgatc ccgttgttgc gcagggcgcg aatggcttcc agcgcgccgg gtaattctgg
     5521 ggcaagggtg acgatgcgaa caagcgccgg atcggcccag cctaggatct cgtctaaatc
     5581 gatggggcgc atatatgctg catcatgtgc accgcgcttg gctgggttaa gataggggcc
     5641 ttccagatgt acgcccagcg gctgagcgcc tttggcgatt tgcatcgccc ccgcgatctc
     5701 gctcaaccgc accgggtagt ccttaaatgg tgacgtgatt atggtgggaa taaagcttgt
     5761 cacgccagtg gacggcaatc gctccgacac agctgccacg ctggcgccat tcagggtgaa
     5821 gtcataaccg aaagcgccgt tgagctgcag gtcgatgaag cccggcagga tggtccctgt
     5881 ggtctgtatg tctgcctgtg gatctgcacc gcgagaaacc tgggtgatgg tgccattgct
     5941 tatgtggata gtagctttgg agatgacgcc gtcaggtagc aatgctagtc cggttagagt
     6001 cagttcagaa tttctcatag gattcaattt atggaattta cttgatttgg actcaaatat
     6061 tatttatgct ctatggaagc ttaatattat cacatggaat taagcaattg aagcctttat
     6121 tcccagtatt ttcgttttat ggcgcgtaac ctagatcata aaccagtgtt tttaactatg
     6181 gcgttgctac ccatggtaat ggaaaaagta tttctcttca aggccgaagg gtatgatcct
     6241 agatggattg aggcagggat caagcaagca gctcaagcct tgctagtttc tctgccagcg
     6301 caaggggacg tgtttttgca cgtcgattgc ccttgggctg attcgcgttt cgcacctcat
     6361 gcgcacacgc acccagctat gatcgaaggc gttgcgaaag cactttcagg cgcatcgcta
     6421 agcttgggcg gttgcagttt gccgtatttt cctacacgct atagtttcaa gcaagccggc
     6481 tatgatgctc ttgcgcgtaa gctcgatgcg gagctggttg catttgagga ggtggcgacg
     6541 cgtcaggtcg aactgggtgg caccgctgtt gtgaataagc aggtgggctt gccttctgcc
     6601 tgggatcagg cagctttccg aatctccctg cccaagctga ggtgcagcac gctggtctct
     6661 tttgccggtg ctctacggca tatgcagagc ttgctgtcac aagctgccca ggtcacggac
     6721 agccatcgcc taccggagaa gatggtagat ctcctgccca cggtgtcccc taatctcatc
     6781 gtagtggatg ctatccatgc gcttcataag ggtggtgaac tctccggtga gccagtggag
     6841 ctggggttga tcatcatcgg cacacatcca cttgcggtag atgtggtctg cgctgtgctc
     6901 ctgggcctcg agccaccccg ggtcgatttc ttgggtgagg ctgcctcacg cggtcttggt
     6961 ccttccagcc tagatgagat cgaattgctg ggtgatctga cccttgatga agtgcgttca
     7021 ctgtcccaga aagtgcaaat ggttgaccct gatccagtgc actatgtatt gccc
//
LOCUS       DBNHOH010000542         4616 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_609312, whole genome shotgun sequence.
ACCESSION   DBNHOH010000542 DBNHOH010000000
VERSION     DBNHOH010000542.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_609312"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="AC16V7_09420"
     CDS             complement(<1..452)
                     /locus_tag="AC16V7_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifH"
                     /protein_id="HIR6257381.1"
                     /translation="MMAWQDQLHNDSLPWLLEPDSPGVRYLALRDLVGLPHDDPALLT
                     ARAAAHTQGPIATILSEMDESGYWAKSGPGYLPKYRSTVWSIIMLAQLGALAEDDPRL
                     ERACAYLIDHALASGGQFSASGAPSGTADCLQGNMCTALLDLGYDDPHL"
     gene            complement(454..954)
                     /locus_tag="AC16V7_09425"
     CDS             complement(454..954)
                     /locus_tag="AC16V7_09425"
                     /inference="COORDINATES: protein motif:HMM:NF019632.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR6257382.1"
                     /translation="MPNPTTKRELLDEIHAEREKLDRFLAPMTPEQMIQPGAIEEWSV
                     KDVLAHLAEWEVLLLIWYNAGRRGENPPLPAEGYGWEQEDDLNQNIYEKYRGWSLDEV
                     LAYYRKSFQETVAAVQAMTEEELFTPGRYAWTKNNTLADYVVPCTSEHYQWARQEMST
                     GQKPGR"
     gene            complement(1243..1872)
                     /locus_tag="AC16V7_09430"
     CDS             complement(1243..1872)
                     /locus_tag="AC16V7_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008099432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HIR6257383.1"
                     /translation="MKKLDLRKELKHLYNPSAKRVEVVEVPRFKFVMIDGAIEPGHEP
                     GNSPKFQEAMQALYGAAYTLKFMSKLRKEDPVDYPVMALEGLWWVENGQFSFTRKDNW
                     MYTLMIMHPDHITQAMFEDAVQQVRAKRGDSPALSLLRWEEFEEGLCIQTMHIGPYAT
                     EPATLEKMDAFARENNYTLHGKHHEIYLGNPQRAQPEKLKTVLRHPISK"
     gene            2202..2759
                     /locus_tag="AC16V7_09435"
     CDS             2202..2759
                     /locus_tag="AC16V7_09435"
                     /inference="COORDINATES: protein motif:HMM:NF021886.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR6257384.1"
                     /translation="MSLKYAMLGFLSYQPFSGYELKATFDDSVRHFWPADQAQIYRTL
                     AELTKDGWATMEVVPQEDRPNKKVYYITEAGREALRGWLAKPLPLQDHRSAELIQVFF
                     AGQLTDEQALAMFERFAQEYRAGLASLYQIPEQIKAYEDMVGSSREAFFWMLTLEHGI
                     HSTRAVLEWLESVIERLKNKQVPQG"
     gene            2785..3789
                     /locus_tag="AC16V7_09440"
     CDS             2785..3789
                     /locus_tag="AC16V7_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium chain dehydrogenase/reductase family
                     protein"
                     /protein_id="HIR6257385.1"
                     /translation="MKYKRVVVSRYGGPEVLQIVEDELPEPQPGEVRLRILAAGVAWG
                     DVLKREGFSIGVRPPFTPGYDIVGVVDKLGEGVSTFKVGQIAAALPLFGGYAELICLP
                     ALELVQVPSGLEPAKAVCLVMNYVVAHQLLHRVARVKAGERILIHGAAGGVGTALLQL
                     GKLAELEMYGTASGGKHETITSLGGIPIDYKTEDVLGRILDLTGDGVDVIFDPVGGSQ
                     TWQSYRMLRRGGRLVIYGAHSILTDGKLKTITGFVLGPILNLLPDHRAVMSYNITRPK
                     YSKPEWCREDLSKLFALLSQERIEPVIAERMPLLEAARAHQLLEEGAVVGKLVLVPNP
                     "
     gene            3951..>4616
                     /locus_tag="AC16V7_09445"
     CDS             3951..>4616
                     /locus_tag="AC16V7_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014649962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR6257386.1"
                     /translation="MEKLLTTKLYIPRTRPDLVPRPRLIERLNEGLHRKLTLICAPAG
                     FGKTTLISEWVAGCERPVAWLSLDEGDNDPIRFHAYLVAALQTIKAGIFENVQAMLRI
                     SQPPPIESVLTELINEVVTLSQDFVLVLDDYQVIEDRSIDDSLTFLLDHIPPVMHLVI
                     ISRSDPMLPLSRLRARSQMTELRIGDLRFSTHEATDFLNRVMGLVLSEEDIAALETRT
                     EGWI"
BASE COUNT         1022 a         1204 c         1240 g         1150 t
ORIGIN      
        1 agatgcgggt catcgtagcc caagtcgagc agggcagtgc acatattgcc ttgcagacag
       61 tcagcagtgc cagaaggcgc cccgctggcg gaaaattgcc cgccggatgc cagggcatga
      121 tcgatcaggt aggcacaggc gcgttccagg cgcgggtcat cttcggctag cgcgcccaac
      181 tgcgccagca tgatgattga ccagactgta gagcgatatt tggggagata accagggcct
      241 gactttgccc agtatccgct ctcatccatc tcggatagga tggttgcgat aggtccttgt
      301 gtatgcgcgg ccgctcgggc ggtcaatagc gccgggtcat cgtggggcag cccgactaga
      361 tcgcgcaagg ccaggtagcg gactccgggg gaatcgggct ccaatagcca aggaagagag
      421 tcgttatgta actggtcttg ccatgccatc atctcacctc ccgggctttt gccctgtgct
      481 catttcctgg cgtgcccact ggtaatgttc gctcgtgcaa ggtactacat aatccgccag
      541 tgtgttgttc tttgtccagg cataccgccc gggggtgaac aactcttctt ctgtcatggc
      601 ctgcacggct gccacagtct cctggaagga tttgcggtaa tatgccagca cttcgtccag
      661 cgaccagcct cggtattttt cgtagatatt ctgattgagg tcatcctcct gttcccatcc
      721 atacccttca gcagggagag gtgggttttc gccgcgccga ccggcgttgt accagatcag
      781 gagcaagacc tcccactcag ccaggtgagc aagcacatcc ttcaccgacc actcttcaat
      841 agctccgggt tggatcatct gctctggcgt catgggtgcc aggaatctgt ccagtttctc
      901 tcgttcagcg tggatttcgt ctaagagttc acgcttcgtg gttgggtttg gcatactttc
      961 ctcctgagat tttttcagca gcttaaatta cttttctcac gtgcttggcc tggatgctga
     1021 tcaaatctag catgcgcttt tccaggacgt cctttggaag gcaggtttgc aaatgacaca
     1081 ctccaacttg gtcatggcga ggagcgtaaa gactaagcca tcttaatgtc cgagatcgcc
     1141 acgccctggc aaagtgcgcc aggcctcgcg atgtccatca ataccgctcg cgcctgaagc
     1201 atgctgcgtt agggatgctc gtgatgacca cggaacagct tttcattttg atattgggtg
     1261 cctgagcacg gtcttgagct tctcgggctg tgctcgctgt gggttgccca ggtagatctc
     1321 atggtgtttg ccatgcaggg tatagttatt ttcccgggcg aaggcatcca tcttctctaa
     1381 cgtggctggc tcggtagcgt agggaccgat gtgcatggtc tgtatacaca agccttcctc
     1441 gaattcctcc cacctcagaa gactgagggc agggctatcg ccgcgtttcg cccgcacttg
     1501 ctggacggcg tcctcgaaca tggcctgggt gatgtgatcg gggtgcatga tcatcagggt
     1561 atacatccag ttgtccttgc gggtgaagct gaactggcca ttctcgaccc accaaagccc
     1621 ttccaatgcc atgacaggat agtccacagg gtcttctttg cgcagcttgg acataaactt
     1681 caatgtgtag gcagcgccgt acaaggcctg catggcttcc tgaaatttag gagagttacc
     1741 tggctcgtga cccggctcga tcgccccgtc gatcatcacg aatttgaagc gcggcacttc
     1801 caccacctcc acacgcttgg ctgaggggtt gtacaagtgt ttcagctctt tgcgtaaatc
     1861 cagctttttc atggtgatct ctcctgcaag cagttccaag tttgcagccg gtgctacggc
     1921 agcatgatgg cggtgttcag ccaattgcgg tgttgttcac aatcatcttt aattggattc
     1981 tacgccaaat tttgctcgag tcccaggtta attatcgaat cagtttttcc tcttgacaag
     2041 atatcatttt tgatatatga ttagacatat ataatataga taatatatga gatacatcta
     2101 atgatttgtc tcgaagtatt gtgatgtgaa gtgcatccat tcaggcaaga atagccagta
     2161 aaaccgaaaa ttatgataca aaccaccaat cggagttacc catgtctcta aaatacgcca
     2221 tgctgggctt ccttagctac caaccgttct ctggctacga gctcaaggct acctttgacg
     2281 attccgtacg tcacttctgg ccggctgacc aggcacaaat ctatcgcacc ctggcagagc
     2341 tgacgaaaga tggctgggcg accatggaag tagtacctca agaagatcgt cctaataaga
     2401 aagtctatta cattaccgaa gcagggcgag aggcgctgcg cggctggctg gctaagcccc
     2461 tgccgctgca ggatcatcgc agcgctgagc tgatccaggt atttttcgcc gggcagctca
     2521 cagacgagca ggccctggcg atgttcgagc gtttcgccca agagtatcgc gctgggctgg
     2581 cgtccttata ccagatacct gagcagatca aagcttatga ggatatggtt ggctcgtcgc
     2641 gggaggcttt tttctggatg ctcacattgg agcatggtat ccactctacc cgcgctgttt
     2701 tggaatggct ggaaagcgtg atcgagcgat tgaagaacaa gcaggttcca cagggatagt
     2761 cggctaacac ttggaggata aaaaatgaaa tataaacgcg tggttgtatc ccgctatggc
     2821 gggccagaag ttctccagat cgtcgaggac gagcttccag agcctcaacc gggcgaggtg
     2881 cgcctgagga ttctggcagc aggcgtggcc tggggagacg tattgaagcg ggaggggttt
     2941 agcatcggcg tacgcccacc gttcacgcca ggctacgata ttgtcggtgt cgtggacaag
     3001 cttggtgaag gggtctcaac gttcaaggta ggacaaatcg cggctgcctt acctctattt
     3061 ggcggctatg ctgagttaat ttgtttacct gccttggagc tggtacaagt gccctcgggc
     3121 ttggaacctg ccaaagctgt ttgcctggta atgaattatg tggttgcgca ccaattgcta
     3181 caccgcgttg ctcgcgtgaa agccggcgag cgcatcttga tccacggtgc ggcagggggc
     3241 gtgggaacag ccctgctcca gctaggtaag ttagccgaac tggaaatgta tggaacagcc
     3301 tccggcggca aacacgaaac gatcaccagc ttgggtggaa tcccaattga ctacaagaca
     3361 gaggacgtct tggggcgcat cctggatttg accggggacg gggttgatgt gatattcgat
     3421 ccggttggcg gcagccaaac ctggcaatcc tatcgaatgt tgcgcagggg cggtcggttg
     3481 gttatatatg gggcgcattc gattctcaca gatggcaagc tcaagaccat cacgggtttt
     3541 gttttgggtc caatattgaa tctcttacct gaccataggg cggtaatgag ctacaacatc
     3601 acaagaccaa aatatagcaa acccgaatgg tgccgtgagg atctatccaa gttgttcgcc
     3661 ctcctgtcac aagaaaggat cgagccggtt attgccgaga gaatgccatt gctcgaggcg
     3721 gcccgggctc accaattgct tgaagaaggc gctgtggtgg gcaaactggt cctggtaccc
     3781 aacccatagg cagcccgcta gcccgcccta tatcttcaca agcggctttc tcgttcttcc
     3841 gtgcctcaga tttatcacat attgttgatg tttgcaattg agcgtaattg aaacctttat
     3901 gagattcgtc atgctataat cattttaaac cacgaaaatc aaggctaatc gtggaaaaac
     3961 ttctaaccac aaaactctat atcccccgaa cccgtccaga cctcgttccg cgcccgcggc
     4021 tgatcgagcg attgaacgag ggtctgcacc gcaaactgac acttatctgt gcccctgccg
     4081 gctttggtaa aaccacactg atcagcgaat gggtcgcagg ttgcgagcgt ccggttgcct
     4141 ggctgtcgct ggatgaaggg gataacgatc ccatccgctt tcacgcttat cttgttgcag
     4201 ccctgcagac cattaaagca ggtatctttg aaaatgtcca agcgatgctc cgtatctcgc
     4261 agccaccacc cattgaatcg gtgctgacag agctaatcaa tgaggtagtt accctttccc
     4321 aggattttgt attagtcctc gatgactacc aggttattga agaccgttca attgacgact
     4381 cactcacttt cttgctcgac catattccac ctgtgatgca cctggttatc atcagccgct
     4441 cagaccctat gctgcccttg tcccggttgc gcgctcggag ccagatgacc gaattgcgca
     4501 ttggcgatct gcgtttttcg acccatgagg caactgattt tctcaataga gtgatggggt
     4561 tggtgttatc tgaagaagat atcgcagcct tggaaacccg caccgaagga tggatt
//
LOCUS       DBNHOH010000543         2524 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1314132, whole genome shotgun sequence.
ACCESSION   DBNHOH010000543 DBNHOH010000000
VERSION     DBNHOH010000543.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1314132"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1230
                     /locus_tag="AC16V7_09450"
     CDS             <1..1230
                     /locus_tag="AC16V7_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HIR6257387.1"
                     /translation="LGVWIAILNLFLAKDRKFHELSKYRWFILQGVIVWVGFEMIRAT
                     FIPVIATSAFIGYTQATQTWLIQPVSIFSVYALNLVIILFNYALAQGIMAWLDRKWTA
                     ADVVPVDADSTRRWLVGTSVVLAVWIGLSLVIVNAMPDTPKLRVASVRPNFPLPAHLD
                     DVNTSQIRFDTFVAQAREAAAQGAQVIYTPEMMFNFDPQVEYTEQFRALAKETGAYIF
                     ITYSVSIEGQDWRNESVMLSPEGEFSQVYGKYHTWMIGEPPTPSAGVFPVYETPLGKL
                     AGMICHDANYTDVARKLTANGAQIIAAPYREFGGFGEQAWTNALFRAVENRVAVVVSG
                     VATVSAIVNPDGSIVDLDTDINGSRAILVGDVRLGPGNAPYTSLGDVLGWASLAGYVF
                     FMFFPSVIERRAKKAQG"
     gene            1286..1666
                     /locus_tag="AC16V7_09455"
     CDS             1286..1666
                     /locus_tag="AC16V7_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257388.1"
                     /translation="MATDQTQLEVTEKKVLNKRLESIGWGCFLIMLGGFALVPEAVVP
                     KGLWSIGVGVIMLGLNIARYFSKIKMSGFTTFLGVIALISGVMQLLGVHSFEGAILLI
                     ILGAYLLVKPWFDRRQLFGKAEDS"
     gene            complement(1745..>2524)
                     /locus_tag="AC16V7_09460"
     CDS             complement(1745..>2524)
                     /locus_tag="AC16V7_09460"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
                     /protein_id="HIR6257389.1"
                     /translation="PGVITGKPLGGGGSLGRTEATGYGVIYTVREAMKHLKMDPTKSV
                     AAIQGFGNVSQYAAIGFVEQLGGKVACVSYWDREDRVAHTVSHAKGIDPSFLQSITDI
                     YGAIDKDKARQAGYTIEDGGDWISKDVDVLIPGALEGQINADTVKRISSRVKLVAEGA
                     NGPTTPDADTVLKGNGIFVIPDFLCNSGGVTTSYFESVQNDMNYYWTRDEVLQRLDTK
                     ITQAFNSVVEMAEKEIVYMRDAAYMVAIDRVVKAMELRGWI"
BASE COUNT          504 a          688 c          732 g          600 t
ORIGIN      
        1 ttgggagtct ggatcgccat ccttaacctt ttcctggcca aagatcgcaa gttccacgag
       61 ttaagcaaat atcgttggtt catcttgcaa ggtgtgatcg tctgggtggg ttttgagatg
      121 atccgcgcca cgttcatccc tgtgatcgcg acgagcgctt ttatcggcta tacccaagct
      181 acacagacct ggttgatcca acccgtctcg atttttagtg tctatgccct gaatctggtc
      241 attattcttt tcaactacgc actggctcag ggtatcatgg cctggctcga ccgcaaatgg
      301 acggctgcgg atgttgtccc cgtggatgca gattcgacgc ggcgctggct ggtagggacg
      361 agcgttgttc tcgcggtgtg gattggcctc agcctggtta tcgtcaatgc gatgccagat
      421 acgcctaagc tgcgcgtggc aagcgtgcgc ccgaatttcc cgctaccagc tcacctggat
      481 gacgtcaata cgtcgcaaat ccgctttgat acgttcgtag cgcaggcgcg tgaagcggca
      541 gcgcagggag cgcaggtcat ctatacacct gagatgatgt tcaacttcga cccacaggtg
      601 gagtacacgg agcagtttcg cgcgcttgca aaggagacgg gtgcgtacat cttcatcacc
      661 tactctgtct cgatcgaggg gcaggactgg cgcaacgagt cagtcatgct ctcgcctgaa
      721 ggggagttct cgcaggtgta cggcaagtat cacacctgga tgattggcga gccgcccacg
      781 ccatctgccg gcgtgtttcc cgtctacgag acgccgctcg gaaaattggc ggggatgatc
      841 tgtcacgatg ccaactatac cgatgtcgcc cgcaagctga ccgctaacgg cgcgcagatc
      901 atcgctgccc cgtatcgcga gtttggaggg ttcggcgagc aagcttggac caacgccctg
      961 ttccgtgccg tcgaaaaccg cgtggcggtg gtcgtatcgg gcgtggcgac ggtctcggcc
     1021 atcgtcaacc cggatggcag tattgtggat ctggataccg atatcaacgg ctcgcgggcg
     1081 attttggtcg gcgatgtgcg ccttggcccc ggcaatgctc cttacacatc gctaggcgat
     1141 gtgctgggct gggcttcgct agcgggttat gtcttcttca tgttcttccc atcggtgata
     1201 gaaagacgag ccaagaaggc acagggttaa tagataaggg ctccctgtgt tgagacgcgg
     1261 ccgaattcaa gtaggagatc taaaaatggc tacagatcaa acacaactcg aagtgacaga
     1321 gaagaaggtg ctcaacaagc gcctggagtc gatcggatgg gggtgtttcc tgatcatgtt
     1381 gggcgggttc gccttagtac ctgaggcggt ggtcccaaag ggtctttggt cgataggggt
     1441 gggcgtcatc atgctggggc tgaacatagc gcgctacttc agcaagatca agatgagcgg
     1501 cttcaccacc ttcttgggtg tcattgcatt aattagcggg gtgatgcagc tgttgggagt
     1561 gcactctttc gagggggcga tcctgctcat catcctcggc gcttacctgc tcgttaaacc
     1621 gtggttcgat aggcggcagc tctttggcaa ggctgaggat agctaaactg ttgataaacg
     1681 agcaattggg cggcgatgtc gccgcccaat tgctcgttta tccaatcccc tgccctccga
     1741 taatctatat ccagccgcgc agttccatgg ctttgacgac gcggtcgatg gcgaccatgt
     1801 aggcggcgtc gcgcatatag actatttctt tctcagccat ttcaaccacg ctattgaagg
     1861 cctgggtgat cttggtgtcc aggcgctgca acacctcgtc gcgtgtccag taatagttca
     1921 tgtcgttctg cacactctcg aaatacgagg tggtcacgcc accagagttg cacaggaagt
     1981 cggggatgac aaaaatgcca ttgcccttga ggacggtatc cgcatctggg gtggtgggac
     2041 cattggcgcc ttccgctacc agtttcacgc ggctgctgat gcgcttgacc gtgtctgcat
     2101 tgatctgccc ttccagcgcc ccggggatga gcacatccac atccttgctg atccagtcgc
     2161 cgccatcttc aatggtataa ccagcctggc gggctttgtc tttgtcgata gcgccgtaga
     2221 tgtcggtgat gctctgtaag aaactcgggt caatcccttt ggcatggctg acggtgtgcg
     2281 ctacacggtc ttctcgatcc caataagaga cacaggcgac tttacccccc aattgctcca
     2341 caaagcctat tgcggcatat tgagagacgt tgccaaagcc ttgaatagcc gccaccgact
     2401 tggtggggtc catcttcaga tgcttcatgg cctcacgcac ggtatagatt acgccatacc
     2461 cagtggcttc ggtgcgcccg agggagccgc cgccgcccag cggtttaccc gtaatcacgc
     2521 cggg
//
LOCUS       DBNHOH010000544         1870 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1378461, whole genome shotgun sequence.
ACCESSION   DBNHOH010000544 DBNHOH010000000
VERSION     DBNHOH010000544.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1378461"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="AC16V7_09465"
     CDS             complement(<1..230)
                     /locus_tag="AC16V7_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012725.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6257390.1"
                     /translation="MSDSNSVIAQSAEDFLQKAWEHHSNKQDDAAEVSFRRAIQMSPG
                     LFDAYFGLGLVLKAQGRKQEAIEAFNKVLELL"
     gene            complement(233..1447)
                     /locus_tag="AC16V7_09470"
     CDS             complement(233..1447)
                     /locus_tag="AC16V7_09470"
                     /EC_number="6.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016851 - magnesium chelatase activity
                     [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase ATPase subunit I"
                     /protein_id="HIR6257391.1"
                     /translation="MVDDQQPASNIQSQVNNPAEVDESPPVRSLRELIDKVTGRGIYP
                     AQPSEESGRAEVLPFPFLAMVGQREMKLALTLALINPAVGGVLLIGPRGTGKTTAVRS
                     LVDLLPEVPRSLCYYGCLPEDIEMGGMDAVCPDCARKYAQNETLAQMDRVRLVELPLN
                     ARLEDVVGGLDERASIHQRMRLRRGILAQADRNLLYVDEVNLLEDDVVDALLDAAAQG
                     SYTVRRGPISATYWSRFVLIGSMNPEEGGLRPQIQDRFGLRVIVRPLDESKERLEVYR
                     RVQAHRANPRHVISAYSQETLQAQHEIQLAGESLPKVSIPDEVAAQGLKLIANLQIES
                     LRAEITLFEAARAYAAADGRKQVSPADLREVASLALRLRRSGFMQEYFARQEEEEAEL
                     TGVIDAVFDTGS"
     gene            complement(1450..>1870)
                     /locus_tag="AC16V7_09475"
     CDS             complement(1450..>1870)
                     /locus_tag="AC16V7_09475"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR6257392.1"
                     /translation="NNWGNVHGGWIIKLMDEAGALACIRHAQRRVVTIAIDQTVFRQP
                     IRIGDLVIITAEVSYVGRTSMEAEVQVTAEDPVTGECTHTNTGYFVYVALDDEGKPAN
                     VPPLIPETDVHIARMKAGRARQLRRLNQRKNNPEDAD"
BASE COUNT          479 a          528 c          409 g          454 t
ORIGIN      
        1 tcaagcaact ctaatacttt attaaaagct tcaatcgctt cttgtttacg accttgagct
       61 tttagtacaa gtcccaaacc aaaatacgca tcgaaaagac cgggactcat ttggatagcc
      121 cgccgaaaac tcacttcggc agcatcatct tgcttattac tgtgatgttc ccaagctttt
      181 tgaaggaaat cttcagccga ttgtgctatt acggaattgg aatcgctcat ttttaagatc
      241 ctgtatcgaa aactgcatca attacaccgg ttagctctgc ctcctcttct tcctgacggg
      301 caaagtattc ctgcataaat cctgaacgcc ttaaacgcaa tgccaatgac gcaacctcgc
      361 gcagatcggc gggagagact tgcttgcgcc catcagcagc agcataggct cgagcggcct
      421 caaataaggt gatctcagca cggagagatt caatctgcaa attggcaata agttttaacc
      481 cttgcgcagc cacttcatct ggtattgaaa cctttggtag actctccccc gcgagctgaa
      541 tctcatgctg cgcttgtagt gtctcttggg aataagcaga gataacatgc cgagggttag
      601 cccggtgagc ttgtaccctg cggtaaacct ccaaacgttc cttggattcg tccagaggcc
      661 gcacaatgac tcgcaaaccg aagcggtctt gaatttgggg gcgaagccca ccttcctctg
      721 gattcatcga cccgattaat acaaaacgcg accaataggt ggctgagata ggtccacggc
      781 gcactgtata actgccttgt gcagccgcgt ccagcaaagc atccaccaca tcatcttcca
      841 acaaatttac ttcatcgaca tagagcaaat tacggtcagc ttgtgctaag atgccgcgtc
      901 tcaagcgcat acgctgatgg atactagccc gctcgtccag acctcccaca acatcttcga
      961 gacgggcatt gagtggcaat tccaccaggc gcacgcgatc catttgggcc aaagtctcat
     1021 tttgggcata cttgcgagca caatccgggc aaaccgcatc cataccaccc atctcgatgt
     1081 cttcaggaag acacccatag tagcacaagc tacgcggaac ctctggcagc agatccacca
     1141 agctgcgaac agcggtggtc ttccctgtgc cacgcggtcc gatcagcaac accccaccta
     1201 ccgctggatt gatcaatgcc agcgttaagg caagtttcat ctctcgctgg cccaccatcg
     1261 ccaaaaatgg aaatggcaat acttccgcgc gcccagattc ctctgaaggc tgagcaggat
     1321 aaatcccacg accagtcacc ttatcgatca gctcgcgcaa tgatctcaca gggggagact
     1381 catcaacttc agccgggtta ttgacttggg actgtatatt cgaagcaggt tgttgatcgt
     1441 caaccatttt cagtctgcat cttctggatt attttttctt tgattcaagc ggcgtaattg
     1501 ccgggcacgc ccagctttca tgcgtgcaat atgtacatct gtttcgggga tcaatggagg
     1561 cacattagct ggtttgcctt catcatcgag agcaacataa acaaagtagc cagtattggt
     1621 atgcgtgcac tcaccagtca cagggtcctc cgcagtgacc tggacttctg cctccatcga
     1681 cgtgcgaccc acatagctca cttcagcagt tattatcacc aaatctccaa tgcgaatggg
     1741 ctgcctgaat acagtttgat caatagctat cgtaaccacc cgccgctggg catgacgtat
     1801 acacgcaagg gcaccagctt catccatcag tttgatgatc caaccgccat ggacattacc
     1861 ccaattgtta
//
LOCUS       DBNHOH010000545         1905 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_930385, whole genome shotgun sequence.
ACCESSION   DBNHOH010000545 DBNHOH010000000
VERSION     DBNHOH010000545.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_930385"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="AC16V7_09480"
     CDS             <1..1077
                     /locus_tag="AC16V7_09480"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR6257393.1"
                     /translation="TIQVLLFHLMIIYAGTGSWIYQEGGEDFITGLVGAYFVVVSQAY
                     FMGLFLLVSAYFVPGAYDRKGPARFLKDRLIRLGIPLAFYSWIISPLLVYLDLRVTEG
                     VTLPWWDYFPGLKHEAFIGSGPLWFVETLLIFTLLYALRRRLARPVQGITAPASSFPS
                     NGSIFLFALLLAVASFLVRQWRPIFWNFAPLNLQFPFFAQYIAMFIVGLIAYRRNWLL
                     SLPDSQGRLWLGVGVLMILLFLPMIMAGGMEIEPFLGGWHWQALVYAVWESFVCLGIS
                     IGLIYLFRRYADRQGRLARALSRNAYGAYVIHGLVITVIALAVRDISLYPLLKWALVS
                     LVAIPLCFGLSSLLIKLPIVGRVL"
     gene            1133..>1905
                     /locus_tag="AC16V7_09485"
     CDS             1133..>1905
                     /locus_tag="AC16V7_09485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6257394.1"
                     /translation="MSVRSELILVIGGTGMLGEPVVRCLRGAGFPVRVMTRSPTKARD
                     RFETSVEVVEGDVDDAPSLAEAVRRCWGVHINLRGDFDYDLERRGAINVASAAAKAGL
                     QRISYLSGASVLAENCWYAGTRAKYDAEAAIRGSGVPYSIFKATWFMEALDNLVRTLG
                     GRKRALHIGRQPFAYHWIAVQDYAQQVARAYSIPEAANKSFYVYGPGAYTMRQALQTY
                     CRIAHPDAMHVFMPIWAAWIFAYLGRRQALKAALPFFSY"
BASE COUNT          308 a          516 c          579 g          502 t
ORIGIN      
        1 accatccagg tgctgctgtt ccatctgatg atcatctacg ccgggacagg atcatggatt
       61 tatcaggagg gcggagaaga tttcatcaca gggctggtcg gcgcttattt tgttgtcgtc
      121 agccaggcgt acttcatggg cctgtttctg ctcgtctcgg cttactttgt gccgggggct
      181 tacgatcgca aggggcccgc acgcttcctg aaggatcgcc tgatccgcct ggggattccc
      241 ctggcctttt atagctggat tatcagtccg ctgctggtgt atctggatct gcgcgtgacg
      301 gaaggcgtga ccctgccgtg gtgggactac tttcccggcc tcaaacacga agccttcatc
      361 ggttctggac cgttgtggtt tgttgagacg ctgctgattt ttacgctgtt gtatgcgctg
      421 cggcggcgcc tggctcgccc cgtccaaggt attaccgcac cagcatccag ttttcccagc
      481 aatggttcaa ttttcctgtt tgccttgctc ctggcggtgg ccagcttcct ggttcgccag
      541 tggcgcccga tcttctggaa ttttgcgccc ttgaacctgc agttcccctt cttcgctcaa
      601 tacatcgcca tgtttatcgt cgggttgatc gcctaccggc gcaactggct cctgagcttg
      661 cccgattcgc agggtcgtct ttggctggga gttggcgttt tgatgatcct gttgttcttg
      721 cccatgataa tggctggcgg gatggagatc gagccatttc tcggcggatg gcactggcaa
      781 gctttggtct atgcagtgtg ggagtctttt gtttgcctgg gcatcagcat cgggttgatc
      841 tatctgttcc gccgttatgc ggatcgccag ggcaggttgg ccagggcctt atcgcgaaat
      901 gcatacggcg cttatgtgat ccatggactg gtgatcaccg tgattgccct tgcagtccgc
      961 gatatctcgc tctatccttt gctcaagtgg gctttggttt cgctggtggc tatcccgctc
     1021 tgttttggtc tcagcagcct gttgattaag ctgcctattg tgggtcgagt gctgtaaagg
     1081 taagcggcag gcgaagcagt ctaacccctt gatctaaaat gagaggcttg aggtgagtgt
     1141 tagatcggaa ttaatcctgg tcatcggcgg cacagggatg cttggtgagc cggtcgttcg
     1201 ctgcctgagg ggtgctggct tccctgtgcg ggtgatgacg cgctccccaa ccaaagcccg
     1261 agatcgcttc gagacttcag ttgaggtggt ggagggagat gtggacgatg ccccttcgct
     1321 ggcggaggcc gtgcgccgtt gctggggggt gcacatcaat ttgaggggcg acttcgatta
     1381 cgatctggag cggcgtgggg ctataaatgt ggcctcagcc gccgcaaaag ccggcttgca
     1441 gcgcatcagc tacctttcgg gggcctcggt tctggctgag aactgctggt atgccggcac
     1501 ccgcgccaaa tatgatgccg aagccgccat tcgggggtcg ggcgttccgt attctatctt
     1561 caaagccacc tggttcatgg aggcgctgga caatttggtg cgcaccctgg gggggcgcaa
     1621 acgcgctctg cacatcggca ggcagccttt tgcttatcac tggatcgccg tgcaagacta
     1681 tgctcagcag gtggcgcgcg cctattccat ccctgaagct gccaacaaga gcttctacgt
     1741 ctatgggccg ggggcatata ctatgcgcca ggcgctgcaa acctattgcc gcatcgccca
     1801 tccggacgcc atgcatgtgt ttatgcccat ctgggcagcc tggatattcg cttatctggg
     1861 gcgacgccag gcgctcaagg ctgccctgcc gttttttagc tacat
//
LOCUS       DBNHOH010000546         3306 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_994704, whole genome shotgun sequence.
ACCESSION   DBNHOH010000546 DBNHOH010000000
VERSION     DBNHOH010000546.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_994704"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="AC16V7_09490"
     CDS             <1..808
                     /locus_tag="AC16V7_09490"
                     /inference="COORDINATES: protein motif:HMM:NF013757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR6257395.1"
                     /translation="WFHMMKPTSTPMRSRYNIEALERGLDILALFTSDTPSLSLTQIV
                     EILNINKSTAYRVLSTLEGLNYLERDPITRLYRPGLKVLQLGFTAISNLDVREVAHPF
                     LVKLSQDLGETVSLAVLDGLYTVYVDRVRNQAIVGVVLGVGSRLPAHCSSLGKVLLSD
                     LPTDELERRLAQAELTSFAPNTISNQQDLISELSKVREQGYAIGDEELAVGLRAAAAP
                     IRDINHRIVAAINATGATINISYERLNNEIIPALIETAHQISITLGYTPN"
     gene            829..2040
                     /locus_tag="AC16V7_09495"
     CDS             829..2040
                     /locus_tag="AC16V7_09495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6257396.1"
                     /translation="MDFNLSEEHLQVQKVVHDFCQKEVAPIIKEADRTQTMHPSILPR
                     MAELGILGICIPEKYGGQGFDYITLGLVCEELEAMDTHLRVVMSVHMGLNSMALLQWG
                     TEEQKQKFLVPQARGEKYAMFGLTEPGVGSDVANMASTARRDGDSYIINGEKMWISLA
                     TRAHNILWVARTNPERPDPHDQLSAFIIETDRPGISVGDIHGKLGVRAGSTGWVSCQD
                     VRVPSSNRLGEEGEGFKIAMSCLDNGRYTVGAGAVGLIRASLEASVKYAKERKAFGKE
                     ISKHQLVQQKIATMVRDYEMGRLQYLQVGWMKNMGMRNTRETSLLKWFATDASFEAAH
                     QAIQIHGAYGYSDEYDVERYLRNARGAIIYEGTSEIHQLIQAGYALGYRKDSKLRCEL
                     PAYDEKEWRTS"
     gene            2048..3244
                     /locus_tag="AC16V7_09500"
     CDS             2048..3244
                     /locus_tag="AC16V7_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR6257397.1"
                     /translation="MSPSALAGLRVIDFSRVLAGPYCTMLLADLGADVIKVEHPQGGD
                     DTRQWGPPWLDGESAYFLSVNRNKRSLTLNLKHPKGRELAHQLAISADILVENFKVGT
                     MAKYGLDYETLSQQNPGLIYCSITGYGQNGPDYDRAGYDFIVQAEAGLMSIIGPPEGP
                     PYKVGVAIVDITAGMFASHAILAALYSRQQTGRGQMIDIALLDSQIAWLANVAQNYLV
                     SGQAPKRYGNAHPNIVPYEVFQTSDGYLALGVGNDKQYQRLCDVAGRTDLRDDARFQT
                     NPGRVTFRDELIPKLQEVFRSRPTATWIEQLSAMGIPVGPINDIPTVMQHPQVQARQM
                     VQIIQRPDGKDVPQLGPVAKYSATPARIYRHPPALGEHSQEILRQDLNLADHEIEELI
                     LEKVIA"
BASE COUNT          853 a          886 c          860 g          707 t
ORIGIN      
        1 ttggtttcac atgatgaaac ccacttcaac ccccatgaga tcgcgatata acatagaagc
       61 cctggagcgc gggctggata tcctcgcgct cttcacctca gataccccct ccctttcctt
      121 aactcaaatt gttgaaatcc tgaacatcaa taagagcacg gcttaccgcg tgctctccac
      181 cttggaaggg ctcaactatc tggagcgaga tccaattacg cgtctctacc ggcctggttt
      241 aaaagttctc cagcttgggt tcacagccat cagcaacctc gatgtgcgtg aggtggctca
      301 cccgttcctg gtgaaattgt cgcaagattt gggtgaaaca gtgagcctgg cagtattgga
      361 cggtctttat acggtctatg tggatcgcgt tcgcaaccag gccattgtag gggtagtttt
      421 gggggtgggg tcccggctac cagctcattg ctcttccttg ggtaaggtgc tgctcagtga
      481 cctacccacc gacgaattgg agcgacgcct cgctcaagct gaattgacat ctttcgcccc
      541 caacacgatc agtaaccagc aggacttaat aagcgaattg tccaaagtga gggaacaagg
      601 ctatgccata ggagacgagg aactggcggt tggattgcgt gccgccgcag cccctattcg
      661 ggatatcaat caccggattg tggcagccat caacgcaact ggggcaacca tcaacattag
      721 ttatgaacgc ctcaacaatg aaatcatacc ggctctcata gagaccgctc atcaaatatc
      781 cattacccta gggtacacac caaattgaag cataaaaagg attaagctat ggatttcaat
      841 ctctctgaag aacatctaca agtgcaaaag gttgtccacg acttctgcca aaaagaagtc
      901 gcaccgatta tcaaagaggc tgaccgcacc caaaccatgc atcccagcat tctaccgcgc
      961 atggcagaac tgggcatctt gggcatctgc atacccgaaa aatacggggg gcaaggtttc
     1021 gattacatca cgcttggcct ggtttgcgag gaattagaag ccatggatac ccatttgcgg
     1081 gttgtgatgt ctgtgcacat gggactgaac agcatggcgc tcctccagtg gggcaccgag
     1141 gagcaaaaac aaaaattcct cgtgccacag gctcgcggtg agaagtatgc catgttcgga
     1201 ctcaccgaac cgggcgtggg cagcgacgtg gcaaacatgg cgtccaccgc tcgccgcgat
     1261 ggagattctt acatcatcaa cggtgagaag atgtggattt cactggctac cagagcccac
     1321 aatatcttat gggtggcgcg aaccaaccct gagcgaccag atccgcatga ccagctctcc
     1381 gcttttatta tcgaaaccga ccgaccgggt atttcggttg gggatatcca cggcaagctt
     1441 ggcgtgcgtg cgggctccac cggctgggta agctgccagg atgtacgcgt gccctccagc
     1501 aatcgcctgg gcgaagaagg agaaggcttc aaaatcgcca tgtcctgcct ggataacggt
     1561 cgttataccg tgggagccgg agcagtcggc ttgatccgtg ccagcctgga agccagcgtg
     1621 aaatatgcca aggagcgcaa agcctttggt aaagagatca gcaagcacca gcttgtacag
     1681 cagaaaattg ccacgatggt gcgggattat gaaatgggtc gcctgcaata cctgcaggta
     1741 ggctggatga aaaacatggg catgcgtaac acgcgcgaga catctctgct caagtggttt
     1801 gccaccgacg cctcgttcga ggccgcccat caggccatac aaatccatgg cgcctatgga
     1861 tacagcgatg aatatgatgt tgaacgctat ctgcgcaacg cacgcggcgc cataatttat
     1921 gaaggcacca gtgaaatcca ccaactcata caggctggct atgctctagg ctaccgtaag
     1981 gatagcaagc tgcgctgtga actacctgct tacgacgaaa aagagtggcg aacttcgtga
     2041 gtattcaatg agcccatcag cgctggctgg tttacgtgtg attgacttct caagggtgct
     2101 ggcaggtccc tactgcacca tgctcttagc cgacctcggg gctgatgtaa tcaaggttga
     2161 gcatccgcag ggcggtgatg acacacgcca atggggacct ccctggttgg atggcgagag
     2221 cgcctatttc ctgagcgtga accgcaacaa acgcagcctg acactgaacc tgaaacaccc
     2281 aaaagggcga gagctagctc accagttggc gatcagcgca gatattttgg tagaaaactt
     2341 caaagtgggc acaatggcga agtatgggct ggattatgaa accctgagcc agcaaaaccc
     2401 agggttaata tattgcagca tcaccggcta tgggcaaaat gggccagact atgaccgtgc
     2461 cgggtatgat tttatcgtcc aggctgaggc agggctgatg tccatcattg gcccaccaga
     2521 agggcccccg tataaagttg gtgtggcgat tgtggatata accgccggca tgttcgccag
     2581 tcacgccatc ctggctgcgc tgtacagccg ccaacaaact ggccgcggtc aaatgattga
     2641 tatcgccttg ctggattctc agatcgcctg gttagccaat gtagcccaaa attaccttgt
     2701 ctctggtcag gctccaaagc gctatggcaa tgcccacccc aacattgtcc cctatgaagt
     2761 ctttcagaca tcagatggct acctagccct tggcgtaggc aacgataaac aatatcaacg
     2821 gctgtgtgat gtcgctggac ggactgacct gcgggatgat gcccgctttc agaccaatcc
     2881 agggcgggtt acatttcgcg atgagttgat accaaagtta caggaagtct tccgaagccg
     2941 ccccaccgca acctggatcg aacaactcag cgcaatgggc atacccgtgg gtccgatcaa
     3001 cgacatcccc accgttatgc aacaccctca ggtacaggct cgccaaatgg tccaaataat
     3061 ccaacgaccg gatggaaaag acgttcccca gcttggacca gtagctaaat attctgcgac
     3121 accagcacgc atctaccgcc accctcccgc cttaggagag catagccaag agatcttacg
     3181 ccaggacctc aacctggctg atcatgagat tgaagaattg attctagaga aagtgattgc
     3241 gtaattcctg agcgagatca tctatgacag aaaagtttta tcacctgatc aacggcgaaa
     3301 aagttg
//
LOCUS       DBNHOH010000547         2329 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_288824, whole genome shotgun sequence.
ACCESSION   DBNHOH010000547 DBNHOH010000000
VERSION     DBNHOH010000547.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_288824"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="AC16V7_09505"
     CDS             complement(<1..458)
                     /locus_tag="AC16V7_09505"
                     /inference="COORDINATES: protein motif:HMM:NF015666.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HIR6257398.1"
                     /translation="MTLDELKIKIQAKEARLAVIGLGYVGFPVACEFARLGFDVLGVD
                     LRRERVDMINSGQSPIVGNEPGLSELAAEVIGKQRFKAVFDYEQMADRDVIIICVETP
                     VDEHNIPRYTALRSALNQLGPVLKDGALVIVESTIAPRTMQDVVLPNLEQA"
     gene            complement(445..1461)
                     /locus_tag="AC16V7_09510"
     CDS             complement(445..1461)
                     /locus_tag="AC16V7_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HIR6257399.1"
                     /translation="MERLRVAVIGTGSIGRNHARVFSELPEVELAAVCDTDSSLVNDI
                     ADRYRTRAFTNYQEMLEKIKPDAVTIAVPTASHIEVAMAALEAEVDLLVEKPIAATLD
                     EGRKLIEKARSKSRLLMIGHIVRFNPAIQELKRRLQSGEVGRIFQIICRRVGPFPARI
                     RDVGVVVDLAPHDLDMMRFLTDMEPIRVFCETEQRIHTDHEDLLLGLLRFPEGITAAI
                     EINWLTPTKVREIIVLGERGMFRVDDLTQDLYFYENAEVGEKLWPALQNLKGVSEGSM
                     TRYAVKRYEPLKAELSAFVRAIKDNTPSPVSGEDGLAALRLALALVESGLKHQVIEVL
                     HDAG"
     gene            complement(1475..1993)
                     /gene="nusG"
                     /locus_tag="AC16V7_09515"
     CDS             complement(1475..1993)
                     /gene="nusG"
                     /locus_tag="AC16V7_09515"
                     /inference="COORDINATES: protein motif:HMM:NF014417.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0031564 - transcription antitermination
                     [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIR6257400.1"
                     /translation="MTDYWYALRSKPRKEEVVWRQVRTQGIEVFYPRLRVQPVNPRSK
                     KWRPYFPGYMFVKVDIDQIGLSSFQWMPHAIGLVCFGDEPAIVPENLIFAIKKRVDEI
                     AAIGGEVFDGLNPGDIVMIDGGPFTGYEAIFDARIEGSERVRVLLQLLNDNRQVPLEL
                     DATQIKQKKRSK"
BASE COUNT          606 a          622 c          490 g          611 t
ORIGIN      
        1 gcttgctcca aattggggag tacgacatct tgcatcgttc ttggagctat ggttgattca
       61 acaatgacga gtgcgccatc tttaagcacg ggtccaagtt ggtttagagc ggatcgcaag
      121 gccgtgtagc gagggatatt gtgttcatcg acgggggtct caacgcatat gatgatcaca
      181 tcccgatctg ccatttgctc atagtcaaac acagccttaa acctctgctt gccgatgact
      241 tccgcggcaa gctcacttaa cccaggctcg ttgccaacaa tgggcgattg tccagagttg
      301 atcatatcaa ctcgctcccg gcgtaggtca acccccaata catcaaaccc aagacgagcg
      361 aattcgcagg caactggaaa tccgacataa cccagtccaa tgacagctaa acgcgcttct
      421 ttggcttgga tttttatttt gagctcatcc agcgtcatgc agcacctcta tcacctgatg
      481 ttttaagcct gactcaacca aagccagcgc cagccgtaaa gccgcgagac catcctctcc
      541 gctgactgga gatggggtgt tatcttttat tgcgcgtaca aaagccgaca gctcagcttt
      601 gagtggctcg tagcgcttga cagcgtaacg cgtcatgctt ccttcactga cacctttgag
      661 attctgtaga gcaggccaga gtttttctcc cacttccgca ttttcgtaaa aatatagatc
      721 ttgggttaaa tcatcaaccc gaaacatccc acgctcaccc aatacaataa tctcgcgaac
      781 tttggtcgga gtcaaccagt tgatctcaat ggccgcggta atcccttcag ggaagcgcaa
      841 taaacccagc agcaaatctt catgatcggt gtgaatgcgc tgttccgttt cacagaatac
      901 acggatgggc tccatatctg tgagaaagcg catcatatcg agatcatgtg gagccagatc
      961 aaccaccaca cccacgtcgc gtatgcgcgc cggaaatggg ccaacacgcc ggcagattat
     1021 ttgaaagata cgaccaacct caccactttg taatctgcgt ttgagttctt gaatggcggg
     1081 gttaaatcgc acaatatgac caatcatcaa caagcggctt ttagaacggg ctttttcaat
     1141 gagtttgcgt ccctcatcca atgtagcagc aatcggtttc tcgaccaaca aatctacctc
     1201 ggcttccaac gctgccatgg cgacctcaat atgagaggct gttgggacag caatggttac
     1261 tgcatccggt tttatttttt cgagcatctc ttgatagttc gtaaaagccc tcgtcctgta
     1321 gcgatcagcg atgtcgttca ccaaagatga atccgtatcg cataccgcag caagctccac
     1381 ctctggcagc tcactgaaga cgcgggcatg gttacgccca attgagcccg tcccgataac
     1441 ggctactcgc aatctttcca tgaagattcc tctattattt tgatcgcttc ttttgtttaa
     1501 tttgcgtggc atcaagctca agcggtacct gtctgttgtc gtttagcaac tgcaacagca
     1561 cccgcacacg ctcgcttcct tctatgcgag catcaaatat tgcttcataa ccagtaaaag
     1621 gtcccccatc aatcatcacg atgtcgccgg gattcaagcc atcaaatacc tctcctccga
     1681 tagcggcgat ttcgtcaacc ctttttttaa tggcaaagat caggttctca ggcacaatag
     1741 caggttcatc accaaaacaa accaaaccta ttgcgtgtgg catccattga aatgatgata
     1801 gcccaatttg atcgatatcc actttaacaa acatatagcc agggaaataa ggtcgccact
     1861 ttttggagcg ggggttgact ggttgcactc gcaagcgagg gtaaaacact tcaattccct
     1921 gagtccggac ttgacgccag acaacttcct ctttcctagg cttactgcgc aaagcgtacc
     1981 aatagtcggt catattctta cccggtatga gtccaagatt gcatcttcaa ggaggccaag
     2041 gaactcaaaa tctctaaatg ttgaggggca attttctcaa tgatcagctg atcttcgcca
     2101 caagctgctg gtgaaattga agcgcggatt ttgcccgcca tattctcttt attcaatccc
     2161 ccacttcgta acttctggaa taagtgatta aggttacaca tcaataataa ctcgctaata
     2221 tgctcataat tgtaccaata aatgttatag atactattgc agcctcacaa ggcttaccat
     2281 cttgctaggc tatttcatat cctgttaacc gttccagcac ccttcgcat
//
LOCUS       DBNHOH010000548         1680 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1314294, whole genome shotgun sequence.
ACCESSION   DBNHOH010000548 DBNHOH010000000
VERSION     DBNHOH010000548.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1314294"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="AC16V7_09520"
     CDS             complement(<1..310)
                     /locus_tag="AC16V7_09520"
                     /inference="COORDINATES: protein motif:HMM:NF014880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HIR6257401.1"
                     /translation="MSNVFTKWKEGLSKTSKATFGQIASLLGASEITDQTFEELEALM
                     IQADLGVDTSEEILAALWERERREGMTKSDQLREAFREELRKRLSAPPSIQYPAQPMV
                     I"
     gene            386..868
                     /gene="moaC"
                     /locus_tag="AC16V7_09525"
     CDS             386..868
                     /gene="moaC"
                     /locus_tag="AC16V7_09525"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HIR6257402.1"
                     /translation="MNSSLSHIDETGRARMVDVGHKPDTERIAVARGEVHMRAETLAL
                     IRGGALKKGDALSVAQVAGVMAAKRTSELIPLCHPLLLNQILVEFEMDDELPGVRIKA
                     TVRTVGKTGVEMEALTAVSVAALTLYDMAKSAEKTMRIQNIRLIEKHGGQSGDVVQDE
                     "
     gene            861..1583
                     /locus_tag="AC16V7_09530"
     CDS             861..1583
                     /locus_tag="AC16V7_09530"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014448.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030366 - molybdopterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein"
                     /protein_id="HIR6257403.1"
                     /translation="MNRIKVLFFATLRQRAGLQHIFIEVKENTSIQDLKSLLGRQIPA
                     LEPVLQTALASINREFAFNDDLIPPEAEVAFFPPVSGGVELPTIYAITEDQLDLDNVI
                     AQITLPTTGAACIFTGMVRAVTRRLDPHDTIFLEYEAYKPMAEAKMRQVAEEIRSRWP
                     DVEGIAIIQRIGRLLPGTPTVMIACTAAHRDTGVFEAARYGIDRLKEIVPVWKKEVGP
                     AGEVWVEGEYTPERDDTWDSQG"
BASE COUNT          398 a          409 c          460 g          413 t
ORIGIN      
        1 agatcaccat cggctgggca gggtactgga ttgagggagg tgcggatagc cgcttacgga
       61 gctcctctct aaaggcttct cggagctggt cagatttggt catcccctct cggcgctcgc
      121 gttcccacag agcagccagg atttcttcgc tggtatccac tcccaggtcg gcctggatca
      181 tcagagcttc caattcctca aaggtttggt ctgtaatctc gctcgcgcct agcagtgagg
      241 caatctgacc aaacgtggct ttgctggtct tggatagccc ttccttccac ttagtaaaaa
      301 cattgctcat gggctgaatt atagcttgaa attttccaaa gatgtttcaa aagtgtagat
      361 ttctagaata cgatataatt ccgccatgaa ttcttcgctc tctcatattg acgagacagg
      421 tcgtgctcgc atggtggatg tgggccataa acctgatacc gaacgtattg ccgtagcccg
      481 tggtgaagta catatgcggg ctgagacgct ggcgcttatc cgcggcggtg ctctcaagaa
      541 aggcgatgcg ctctccgtgg cgcaggttgc tggggtgatg gcggcaaaac gcacctcaga
      601 actgattccc ctctgtcatc cgctcttgtt aaatcaaatc cttgtggaat tcgagatgga
      661 tgatgagttg ccgggtgtgc gaatcaaagc tacagttcgc acggtgggaa aaaccggcgt
      721 agagatggaa gcactgactg ctgtatccgt cgctgcctta acactctacg acatggcgaa
      781 atctgccgag aaaaccatgc gcatccaaaa tattcgcctt attgagaaac atgggggtca
      841 gagcggagat gtagtccaag atgaatagaa tcaaagtgct gtttttcgcg acgttgaggc
      901 aacgggctgg cttgcagcac atatttatcg aagtgaaaga aaatacgagc attcaagacc
      961 tgaaaagctt gctaggccga cagatacctg cgctggagcc tgttttgcag actgcattgg
     1021 cttcgattaa tcgggagttt gcttttaatg atgaccttat tcccccagag gcggaggtag
     1081 cctttttccc acccgttagc ggaggggttg agcttccgac gatttacgcc attacggaag
     1141 accagctgga cctggacaat gtgattgctc agatcacctt gcccactacc ggagccgcct
     1201 gtattttcac gggcatggtg cgcgcagtta cccgtcgact cgaccctcac gacacgattt
     1261 ttctggaata tgaagcctac aaaccgatgg cggaggccaa aatgcgccag gttgccgagg
     1321 aaatccgctc tcgctggcca gatgtggaag gcatcgccat tatccagcgc attgggcgat
     1381 tactacctgg cacacctaca gtgatgatcg cttgtaccgc ggcacatcgt gatactggcg
     1441 tatttgaggc agctcggtat ggaattgatc gccttaaaga gatcgtcccg gtatggaaaa
     1501 aagaagtagg gcctgctggg gaggtctggg tggaaggtga gtatactcca gagcgagacg
     1561 atacctggga ttcgcagggg taatagacag cggatgaatg cgatccattg cataaattgt
     1621 catcttcaat atccggatac agatataccc tatcgctgcc cgcgctgcgg tggtttgttt
//
LOCUS       DBNHOH010000549         1825 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_801364, whole genome shotgun sequence.
ACCESSION   DBNHOH010000549 DBNHOH010000000
VERSION     DBNHOH010000549.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_801364"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="AC16V7_09535"
     CDS             <1..547
                     /locus_tag="AC16V7_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6257404.1"
                     /translation="LGTLAAAAVYRSNFFGKESVSFLLVLPLALPGIVTGIALRSALG
                     LMEIPFTFWTIVIGHATFCVVVVYNNALARFRRLGHSQIEASMDLGANAFQTFRHIVL
                     PNLATALLAGGMLAFALSFDEVIVTTFTAGQQSTLPIWIFSQLTRPRDRPVTNVVATF
                     VIVITFIPILLAHRLTQQDNV"
     gene            complement(555..1619)
                     /locus_tag="AC16V7_09540"
     CDS             complement(555..1619)
                     /locus_tag="AC16V7_09540"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR6257405.1"
                     /translation="MQITRAEVTPVELKLRQPVALAGHPAIQHITSVFVRIETRQGQS
                     AWGCTIAHPSLTGEKPEQVIHACREYASIASDLHPTNIEYSLSVLTSQVKGDGSALCA
                     FDMAFHDLLGLAAGMPVYRLLGGYRNRIQTSVTIPLATIQESVDIAQARAHLGYRILK
                     IKGGLDPAEDVRRVQAIRRALPDHILRLDADGGYNVQQALDVARALKGKLEMLEQPTP
                     ADDLVSLRQVTEHSPVKILADQSVTGPASALDLAAKRAAGGISIKLAACGGLRCARQI
                     DSIARAARLSTMVSCFIEPALLITAGLSLALSSPNVLYGDLDGNLDLVNDPSKPGFRL
                     EDGWLVAFDTPGLGYSVELN"
BASE COUNT          389 a          492 c          481 g          463 t
ORIGIN      
        1 cttgggtacg ctcgccgcag cggctgtcta tcgcagcaat ttctttggaa aagagtccgt
       61 atctttcctg ttggttttgc cgttggcgtt accgggaatc gtaaccggga ttgccctgcg
      121 ctctgctctc ggtttgatgg aaatcccgtt caccttctgg acgatcgtca ttggccacgc
      181 cacgttctgc gtggtggtcg tttataacaa tgcgctggcg cgattccgcc gccttggtca
      241 ttcccagatc gaggcttcca tggatctggg agccaacgcc ttccagacgt tccgtcatat
      301 tgttttgcca aacctggcga ctgccttgct ggcgggaggt atgctggcct ttgcgctctc
      361 tttcgatgag gtgatcgtca ctactttcac ggctggacaa caatccaccc tgccgatctg
      421 gatcttcagc cagcttacgc ggccgcgcga ccgtccggtg acgaatgtgg tagcgacatt
      481 tgttatcgtc atcaccttta ttcctatcct gctggcacac cgcttgactc aacaggacaa
      541 cgtgtagaga tatctcaatt tagctcgaca gaatagccca aaccgggggt gtcgaaggcg
      601 actagccaac catcttccaa tcgaaaacct ggcttagatg gatcattgac caaatccagg
      661 tttccatcca ggtcgccata aaggacatta ggactgctca aagccaggct gagaccggca
      721 gtaatcagca acgccggctc aataaagcag cttaccatag tcgacaagcg ggcagcccgg
      781 gcgatggagt cgatctggcg tgcacagcgc aatccaccac aggcagccag tttaatgctt
      841 atccctcctg cggctcgctt tgctgctagg tccagggctg atgctggacc agtcacgctc
      901 tggtcggcca agattttcac cgggctatgc tcggtgacct ggcgcagact aaccagatcg
      961 tcggcaggtg tgggctgttc cagcatctcc agcttacctt tcagggcgcg ggcaacatcg
     1021 agagcttgtt ggacattgta accaccgtct gcatccagac ggaggatatg atccggcagg
     1081 gctctgcgga tggcttgcac acggcgcaca tcctccgccg ggtcgaggcc acctttgatc
     1141 ttcagtatgc gatagcccag gtgggcacgc gcctgggcga tatccacgct ttcctggatg
     1201 gtggctaggg gtatggtcac tgatgtttga atgcgattac ggtagccacc caggagtcga
     1261 taaacaggca tgcctgcagc caatcccaat agatcgtgga aagccatgtc gaaggcgcaa
     1321 agcgctgagc catccccttt gacctgactg gttaacacag aaagggagta ttcgatattg
     1381 gttgggtgca gatcagatgc tatgctggcg tattcacggc aagcatggat gacctgctcg
     1441 ggtttctcac ctgtcaagct tgggtgagcg attgtgcagc cccaggcact ttgcccctgg
     1501 cgtgtttcta tacgcacaaa tactgacgtg atgtgctgaa tcgctggatg cccagcgagc
     1561 gctacgggtt gtcgaagttt taattcgacg ggtgtcactt cagctcgggt tatctgcatt
     1621 tgcaaacctc ataacaaagg atatccttat tatacaaaat atctttctat atgctctttt
     1681 gcgttgcttc atcttagcaa ttttccttga cagctatcta tcctgtgtcg ggtacgtcaa
     1741 ttatgcaatt gcaccacctc tatcaaaatt ggcggatttg ggaattggca cttgacaatc
     1801 taggataatg tcgtataatc cagca
//
LOCUS       DBNHOH010000550         5661 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_160061, whole genome shotgun sequence.
ACCESSION   DBNHOH010000550 DBNHOH010000000
VERSION     DBNHOH010000550.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5661)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5661)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5661
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_160061"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            298..1635
                     /locus_tag="AC16V7_09545"
     CDS             298..1635
                     /locus_tag="AC16V7_09545"
                     /EC_number="5.4.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR6257406.1"
                     /translation="MNIFEDQFLSRTPKSQAMFARASRRLPGGVAGNGKFLPPYPIYV
                     EQAQGGEFLDVDGNRYVDLLMGGGVHILGHSPEVVMSAVEGQLRKGAHYYMAAEAEVL
                     LAEKICQLMPHVEMVRFVNTGSEATQMALRAARAYRKRDKIAKFEGNFHGQHEAVLIS
                     TLGAEGEADAPSPRIDSAGVPVQTGAGVLVLPYNDAERATALIEEYADELGAVILEPV
                     SAFGLGVVAGETAFLQAVRQVTEKHGIPLIFDEVVTNFRLGLGGATEYFGVTPDLVCL
                     GKIIGGGFAIGGYGGRREIMEQVVTPRTGMWNLSEQIFQSGCFSGNPISMIAGLAVLS
                     ELEKGQVHPHINAIAEQLQSGLTEIGRRLGFPILVNRAASFFQVHFGVERIRDKRDQL
                     RADKKTADLFHYGLRSHGMMASSHPLFISAAHTGQHVGMVLEVAERVLKQMSA"
     gene            1710..2819
                     /locus_tag="AC16V7_09550"
     CDS             1710..2819
                     /locus_tag="AC16V7_09550"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6257407.1"
                     /translation="MNEFSVELRNVVKQFPTPEGGEVLAVNDVTMRIKNGEFFSMLGP
                     SGCGKTTSLRMIAGFEWPTMGDIYIEGEAMGHRPPYLRPVNTVFQSYALFPHMSVSEN
                     VAYGLEMEKTPKHEIKRRVGEALEMVKLGGMERRKPKQLSGGQQQRVALARALVKRPK
                     VLLLDEPLGALDLKLRKEMQLELKALQQEVGITFVYVTHDQEEALTMSDRIAVMDHGV
                     VLQVGSPVEIYERPNRRFVADFIGETNFLTGEVRVIEENRVTVYIPSLNDELVGTKAA
                     PVRVGEQVVVSMRPEKIRLAADKALPQNCFSGRVVNTTYIGSDSRVIVDLGGVSLTVW
                     EQNKISTLDPEAYYERDEQVWVVVLPENALVLHEG"
     gene            2850..3773
                     /locus_tag="AC16V7_09555"
     CDS             2850..3773
                     /locus_tag="AC16V7_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6257408.1"
                     /translation="MLRKLTEDKSVQGTVLISPTLLFMIGLLIIPLLLTLVISFGSRA
                     PDGTVIYGFSFDNYVRLSGVRTECADGKSICLDPIYIQILSRSLSLAFNTTVLAIMMA
                     YPLAYFIARAPVKKRNTFLFLILVPMWTNFVIRVYAWMMLLRTQGVINSAIGWLAGLV
                     GLPFTPFEMLYTQGAVLVGMIYEFLPFMILPIYTSLEKIELSLYEASADLGASPIRTF
                     LRMTLPLSMPGVVAGTILVFVPVMGTFIVSDLLGGRQIVLVGNLIQNQFLIARDPTFG
                     SAASLVLMTLTLIVTYFYTRKFGFGEEIIAA"
     gene            3770..4651
                     /locus_tag="AC16V7_09560"
     CDS             3770..4651
                     /locus_tag="AC16V7_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6257409.1"
                     /translation="MNPHRRSPLVVVSTVLVYVFLYAPVVVLFLFAFNSSKTNVLFEG
                     FITQVGPTEIDSGNVIQSPCGAFHWFCELAKNIEVRNAARNTLTIAFVSTIISTLIGT
                     LAAIALQRYNFKLKTFSEVSLYIPIVIPEIVMGIGILVLFSQMFTLLNSALSLSGDSQ
                     LTLGLATVIVSHIAFSIPFVTLVVRARLHGFDKSIEEASMDLGANEIITFWRVTLPSI
                     MPGVLSGALLAFTLSLDDFVITFFTTGPGASTLPIYVYGLLRRIVTPQVNALSAVWIS
                     IVLVAVFFTQRLQARAD"
     gene            4771..>5661
                     /locus_tag="AC16V7_09565"
     CDS             4771..>5661
                     /locus_tag="AC16V7_09565"
                     /inference="COORDINATES: protein motif:HMM:NF013697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6257410.1"
                     /translation="MKKTMLWRLLAAMMVLSLLLAACGGGGGAPSAGEAEEFVTGSGF
                     VCPQPNPRVEVTSKELNLFVWTEYIPVEWKECFELVYGIKVNHDEYSSNEEMYAKLSA
                     GATTYDLILPTDYIVSLMVGQGMLQEWDHAKLPMLANIDSGYKDLPFDPGNKYTIPYM
                     AGTDALVYSVERVATPPTSWADLWNPDYAGRLVLLDDPRVVIGFTLPTLGYDLNTKDP
                     AQLEEAKTKLLELIPNVKLFDSDSPKTALIAGDVDVGNVWNGEAFTAQQEDPNFEYVF
                     PSEGTILWQDNWAIPASAPHL"
BASE COUNT         1204 a         1463 c         1610 g         1384 t
ORIGIN      
        1 tatggtatca aggagatgta gactgggatt tgcttacaac ttttgtggat aagactttga
       61 gaccttaaat tgccgaaaga atcgacgtag cagcgttctc tatacgggct tagataaata
      121 ccttatcaag gcatgaaaaa cgagtaaaat cgaaaggata aataataacc tttagaagat
      181 gaatatgccc caatatgcag ttgaaattga cgcaaaactt gtcccattgc gggatcaatt
      241 atggatacag gctgctgaag gtgaaaaact tgacacgagg caaataaaag gcatttgatg
      301 aacatattcg aagatcaatt tctctcccga accccgaaat ctcaagccat gttcgcgcgt
      361 gccagccggc gattgcccgg tggggtagct ggcaacggca aattcttgcc gccctacccg
      421 atatacgtgg agcaggcgca ggggggcgag ttcctcgatg tggacggcaa ccgctacgtg
      481 gatttgctga tggggggtgg cgtgcatatt ctaggccatt cgcccgaggt agtgatgagc
      541 gctgtggagg gccagttgcg caagggtgcg cattattata tggctgctga ggctgaggtt
      601 cttcttgccg aaaaaatctg ccagttgatg ccacacgtcg agatggtgcg cttcgttaat
      661 actggctcag aggccacaca gatggcttta cgggcggcgc gcgcttatcg caagcgggat
      721 aaaattgcta aattcgaggg caactttcac ggccagcacg aggcggtctt gatcagtacg
      781 ctgggggctg aaggggaagc ggatgccccc tccccgcgca ttgacagcgc tggcgtcccc
      841 gtccaaaccg gcgcgggcgt actggtgctg ccttacaacg acgctgagcg cgcgacagct
      901 ctaattgagg agtatgccga tgaattgggc gcggtgatcc tggaacctgt ctcggccttt
      961 ggactgggcg tggttgcagg tgagaccgct tttctccagg ccgtgcgtca ggtcacggag
     1021 aagcatggca tcccgctgat ttttgacgag gtcgtgacca actttcgcct ggggctgggt
     1081 ggggcgacgg aatatttcgg cgtcacgccc gatctggtct gcctgggtaa gatcattggt
     1141 ggcggtttcg ccattggtgg ctacggtggg cggcgcgaga ttatggagca ggtggtcacg
     1201 ccgcgtacag gcatgtggaa tctcagcgag caaattttcc aatcgggctg tttttcaggc
     1261 aatcctatct cgatgatcgc cggattagcc gtgctcagtg aactagaaaa agggcaggtt
     1321 cacccgcaca tcaatgccat cgctgaacaa ttgcagagtg ggctgacgga gattggcagg
     1381 cgcctgggct tccccattct ggtcaatcgg gcggcctcgt tcttccaggt gcatttcggg
     1441 gttgagcgca tccgggacaa gcgtgaccaa ttgcgcgctg ataagaaaac tgccgattta
     1501 ttccactacg ggttgcgttc tcatggcatg atggctagct cgcatccctt gtttatttca
     1561 gccgctcaca ccgggcagca tgtcggcatg gtactggaag tggcggagcg cgtcttgaag
     1621 caaatgtctg cataaactta agtagttgtc gaaaatggga cttcacagtc attttttctg
     1681 gcaacggtgt tcatggtttt ggagtcgaat tgaacgaatt tagcgtagag ttgcgcaatg
     1741 tggtgaagca atttcctacc cccgagggag gcgaggtctt agccgttaat gatgtcacca
     1801 tgcggatcaa gaatggcgag tttttctcca tgctggggcc ttcggggtgc gggaaaacca
     1861 cctcgttgcg catgatcgct ggttttgagt ggccgactat gggtgatatc tatatcgagg
     1921 gtgaagccat ggggcacaga ccgccttatt tgagacccgt caacaccgtt tttcagagct
     1981 atgccctctt cccacacatg tcggtgtccg agaacgtggc ttatggtctc gagatggaga
     2041 aaacacccaa gcacgagatt aagcgacgcg tcggcgaggc cttggagatg gtcaagctag
     2101 gcggtatgga gcggcgcaag ccaaagcagc tttctggcgg gcagcagcaa cgcgtagccc
     2161 tggcgcgcgc cttggtcaaa cgccccaagg tgctgctact ggatgagccg ctgggcgccc
     2221 tcgacttgaa gctacgtaaa gaaatgcagt tggaactcaa agccttgcag caagaagtgg
     2281 gcatcacctt tgtctatgtg actcacgacc aggaagaagc cctgacgatg tccgaccgca
     2341 ttgccgtgat ggatcatgga gtggtgctgc aggtcggatc gccggtggaa atctacgagc
     2401 gccccaaccg ccgtttcgta gccgatttca tcggcgagac caatttcctc accggcgagg
     2461 tgcgcgtcat tgaggagaac cgcgtcacag tttacatccc cagcctgaac gatgaattgg
     2521 tcggcacgaa agccgcgccg gtgagagtag gtgagcaggt ggttgtctcg atgcgcccgg
     2581 agaaaatccg cctggctgca gacaaagccc tgccccagaa ctgcttcagc ggcagggtgg
     2641 tgaacacgac ctacatcggc tcggactcac gcgtgatcgt ggacttgggc ggtgtatccc
     2701 tgacagtttg ggagcagaat aaaatctcca ccctcgaccc ggaggcttat tacgagcgcg
     2761 acgagcaggt ctgggtggtg gtgctacctg aaaatgccct cgtccttcac gaagggtgag
     2821 gatgctgaaa aaaggataaa aatagtagca tgctgcgaaa gctaaccgaa gataaatctg
     2881 ttcagggtac ggtgttaatc tcgcccactt tgctctttat gatcgggctg ctgattatcc
     2941 ctctgctact aactctggtg atcagctttg gcagccgcgc tcccgatggc acagtgatct
     3001 acggattctc gttcgacaac tacgtacgcc tctctggggt acgtaccgag tgtgctgacg
     3061 gcaaatctat ctgcctcgat cccatttata tccaaattct atcgcgctcg ttgagtctgg
     3121 ccttcaacac cacggtgctg gcgatcatga tggcctatcc gctggcttat ttcatcgccc
     3181 gtgccccagt caaaaaacgc aacacttttc tctttttgat cctggtaccg atgtggacga
     3241 atttcgtcat ccgtgtctac gcctggatga tgttgctgcg cacgcagggt gtaatcaaca
     3301 gcgcgattgg ctggctggct ggattggtgg ggctgccttt cacgcctttt gagatgctct
     3361 acacccaggg ggcagtgttg gtcggtatga tttatgagtt tttacccttc atgatcctgc
     3421 caatctatac ttcgttggaa aagattgagc tttcgctata cgaggcctcg gcagacctgg
     3481 gggcgagtcc tatccgcact ttcctgcgca tgacgctacc gctctctatg ccaggggtgg
     3541 tggctgggac gattctggtc tttgtgccgg tgatgggcac tttcatcgtc tccgatttgc
     3601 tgggtgggcg gcagatcgtg ctggtcggta acttgatcca gaatcaattc ctcatcgccc
     3661 gcgatccaac cttcggctcg gcagcttcgc tggtgctgat gaccctgacg ctgattgtga
     3721 cctatttcta tacgcgcaaa ttcggttttg gggaggagat catcgcagca tgaacccgca
     3781 ccgccgctcc cccctggtcg ttgtctctac tgtgctggtg tatgttttct tgtatgcacc
     3841 ggtggtggtt ttgttcttat tcgccttcaa ttcctcgaaa accaatgtac tcttcgaggg
     3901 attcatcaca caggtcgggc cgaccgagat cgactctgga aatgtcatcc agagtccttg
     3961 cggcgcgttt cactggttct gcgaactggc gaaaaatatc gaggtccgca acgctgcccg
     4021 caacacactc accatcgcct ttgtctcgac gattatatcg acgctcatcg gcaccctagc
     4081 ggcgattgcc ttgcagcgct acaattttaa attgaagaca ttctcggagg tttcgctata
     4141 tatccccatc gttattccgg aaattgtgat gggcatcggc attttggtct tattcagtca
     4201 gatgtttacc ctgttgaata gtgccctcag cctgagtggt gacagccagc ttaccctggg
     4261 attggctacc gttatcgtct cgcatatcgc ttttagcatc cccttcgtga cgctggtggt
     4321 gcgagcgcgg ttgcatggtt ttgataaatc gatcgaggag gcttcgatgg atttgggcgc
     4381 caacgaaatc atcactttct ggcgcgtgac cctgccctcg atcatgcccg gcgtgctctc
     4441 cggagcgctg ctagccttca ccctctcgct ggacgatttt gtgattacct tttttaccac
     4501 cgggccgggc gcctctacgc tgccgatcta tgtctacgga ttgctgcgcc gcattgtcac
     4561 tccgcaggtc aacgccctct cggcggtgtg gatttcgatt gtgctggtcg ccgtcttttt
     4621 tacgcagcgc ttacaagcga gggcagattg acaatctgtt ttagctatta tggtcttacg
     4681 ggcatatggc aggtatggat acctgccggc gaaggcggtt tcctgaaaac cgctctactt
     4741 tcaatccata tatcttaaca ggaggcagaa atgaaaaaaa caatgttatg gagattgttg
     4801 gcagcgatga tggtactcag cttgctgctg gcagcctgcg gcggtggtgg tggagcacct
     4861 agcgcaggcg aggccgagga atttgtcacc ggctcgggtt tcgtttgccc tcagccgaat
     4921 ccgcgcgtcg aggtcacctc gaaggagttg aatctctttg tgtggacgga gtacatcccg
     4981 gtggagtgga aggaatgttt cgagttggtt tacggcatca aagtgaacca cgacgagtat
     5041 tccagcaacg aggagatgta cgccaaactg tctgctggag ccacgactta cgatctgatt
     5101 ctgcctaccg actacattgt ctcgttgatg gtcggccagg ggatgctcca agagtgggat
     5161 catgccaaac tgccgatgct ggcgaatatt gattccggct acaaagacct gcccttcgat
     5221 cccggcaata agtacacaat cccctacatg gccggcacgg atgccctcgt ctacagtgtg
     5281 gagcgcgtcg ctaccccgcc aacatcctgg gctgatttgt ggaatcccga ctatgctggt
     5341 cgcttggttt tgctcgatga cccccgcgtg gtgattggct tcacgttgcc gaccctgggc
     5401 tacgatctca acaccaaaga tccggctcaa ttggaagagg ctaaaacgaa actgttggaa
     5461 ctgatcccca atgtcaagct cttcgacagc gacagcccca agacggctct catcgccgga
     5521 gacgtggatg tgggcaatgt ctggaacggc gaagcattca ctgcccagca ggaagacccc
     5581 aattttgagt atgtcttccc cagcgaaggc actatcttgt ggcaggataa ctgggccatc
     5641 ccagccagcg ccccgcacct g
//
LOCUS       DBNHOH010000551         1093 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1314352, whole genome shotgun sequence.
ACCESSION   DBNHOH010000551 DBNHOH010000000
VERSION     DBNHOH010000551.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1314352"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1093
                     /locus_tag="AC16V7_09570"
     CDS             <1..>1093
                     /locus_tag="AC16V7_09570"
                     /inference="COORDINATES: protein motif:HMM:NF013799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR6257411.1"
                     /translation="WAVMHSRPPSYSDPSYSVLLTGAWPDLSDGPTFNVPYEETPTLT
                     QDNIFLAAKRNGLNTALSGYYWFEKLVPQQAVDVSFYTPGEDQAADRQVVDAALPWLE
                     GSEHNLVLIHLDQVDYAGHYEGGPRDPRWEAAARRADDLIREIASQLDLSRDTLFVAS
                     DHGQIDQGGHGGHEPVLLLEPFVLVGAGVIPGEYGDVNMVDVAPTLSVLVGANIPAAT
                     QGHVLEEMLTLSEVYKNALPSVTYDQQKQLLEAYQRAIGVEIEQAPPMKTSAGQVVDD
                     FQAALEATRWDRLRPERLGRAVLASALFLLPLVFLIWKRGKDIAFMLVGALFYHVLFH
                     FRYATLDRLPYSLSWVDSQEALIQYIGVTA"
BASE COUNT          226 a          280 c          312 g          275 t
ORIGIN      
        1 tgggcagtca tgcacagccg gcctccatct tactccgacc caagttattc tgtccttttg
       61 acgggcgctt ggcctgattt gagtgatggc cccaccttta atgtgccgta tgaggaaacc
      121 ccaaccctga ctcaggataa tatattttta gctgccaagc gcaacggatt gaataccgca
      181 ttatccggct attactggtt cgagaaactg gtacctcaac aggcagtaga tgtgagcttc
      241 tatacgccgg gtgaagatca ggcggctgat cgccaggtgg tggatgctgc cttgccctgg
      301 cttgagggaa gcgaacacaa tctggtgctg attcatttgg atcaggtaga ttatgccggg
      361 cattatgaag gtggtccgcg cgatccacgc tgggaggctg ccgcccgacg cgccgatgat
      421 ttgatccgtg agatcgccag ccaattggac ctctccagag ataccttgtt cgtcgccagc
      481 gatcacggtc agattgacca gggaggtcat ggtgggcatg agccagtttt gctgctcgag
      541 ccgtttgttt tggttggggc gggtgtcatt cctggggaat atggcgatgt caacatggtg
      601 gatgttgcgc ctacactctc tgtccttgtt ggggcaaaca taccggctgc gactcaaggg
      661 catgtgttgg aggagatgtt gacactcagc gaggtctata aaaacgccct gccatcggta
      721 acatatgacc agcaaaagca gctgcttgag gcatatcagc gcgcaattgg tgtagaaatc
      781 gagcaagcgc ctcccatgaa gacctccgct ggacaggttg tagatgactt ccaggctgcg
      841 ttggaggcca cccgatggga tagactcagg cctgagcgtc tggggcgcgc cgtcttagcc
      901 agtgccctct ttctgctccc attggtattc ttgatctgga aacgcggcaa agatatcgcc
      961 ttcatgctcg ttggggcgtt gttttaccac gtcttgtttc acttccgcta tgcaacttta
     1021 gaccgcctgc cctattcgct cagctgggtg gatagccagg aagccttaat ccagtatatt
     1081 ggcgtaactg ccg
//
LOCUS       DBNHOH010000552         2182 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_544473, whole genome shotgun sequence.
ACCESSION   DBNHOH010000552 DBNHOH010000000
VERSION     DBNHOH010000552.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_544473"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="AC16V7_09575"
     CDS             <1..982
                     /locus_tag="AC16V7_09575"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR6257412.1"
                     /translation="EAEAIRNTGAYTEIIPYDEAISPILRATLERLNPEKIAVNFSRN
                     DVLADGLSHGLYQVLCDHLSGTPFVERFMSAEKVIASLRGRKTEGEIARIRSAIEITD
                     QIFTNTFDYAQVGMTENQISEFMHAQLDQFSLEPAWDLDHCPVVNAGPDSVAGHVGPS
                     QLAIKPGQILHIDFGVKKDDYCSDIQRLVYFLAPGEQKPPAEVQRGFETILQSTQAAI
                     NAMRPGITGKEVDAVAREIITDAGYPEFKHATGHHLGRLAHDGAGTIGPLWERYGDTP
                     NFQLEAGQVYTIEPSLYLPGYGHMGIEEDVLVSENGAEYLTVPQRELILK"
     gene            1067..1417
                     /locus_tag="AC16V7_09580"
     CDS             1067..1417
                     /locus_tag="AC16V7_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257413.1"
                     /translation="MCEFCTEHGEGKAWYLTMKNYSQELLSQNGRIEHIRAFFKDFEI
                     RTAQSLSMLDQIQALPFVPDIVSRVVTSRQKKAHFGQVVPIEDVDRLLDDISSIVRIP
                     CVCRSLTTGRQETR"
     gene            1640..1738
                     /locus_tag="AC16V7_09585"
                     /pseudo
     CDS             1640..1738
                     /locus_tag="AC16V7_09585"
                     /inference="COORDINATES: protein motif:HMM:NF012267.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(1754..1987)
                     /locus_tag="AC16V7_09590"
     CDS             complement(1754..1987)
                     /locus_tag="AC16V7_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257414.1"
                     /translation="MRSNDEYLRWANPDTCSTLTGILAAVYQNSVLSWLGYFPLPPEG
                     PLSLLKMDSVLLAGCPAYTASIAQIGIDQAFVV"
     gene            1991..2110
                     /locus_tag="AC16V7_09595"
                     /pseudo
     CDS             1991..2110
                     /locus_tag="AC16V7_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012267.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
BASE COUNT          579 a          518 c          532 g          553 t
ORIGIN      
        1 tgaagctgag gccataagaa ataccggagc ttatacagaa atcatcccat atgatgaagc
       61 gataagcccc atattacgag cgactctgga gcgtcttaat cctgaaaaaa ttgcagtcaa
      121 tttctcccgc aatgatgtcc ttgctgatgg tttatcccat ggtctttatc aagtattgtg
      181 cgaccacctc tcaggtacac catttgtcga aagatttatg agtgcagaaa aggtgatcgc
      241 gagccttaga gggcgaaaaa ctgaaggaga aatcgctcgt atccgctctg ctatcgagat
      301 aactgatcaa atattcacga ataccttcga ctatgcgcaa gtaggcatga ccgaaaatca
      361 gattagtgaa tttatgcatg ctcaactcga tcaattcagc cttgagcccg cctgggatct
      421 agatcattgc ccggttgtaa acgctggacc agactccgtg gcagggcacg tcgggccatc
      481 acaactcgcg attaagccag ggcagatttt gcacattgat tttggggtaa agaaagatga
      541 ttattgctcc gatattcaac gcctggttta tttcctcgct ccgggtgaac aaaaaccacc
      601 tgcagaagta caacggggtt ttgaaacaat cctccaatcc acacaagccg ctattaatgc
      661 catgcgccca ggcatcaccg gtaaagaggt agatgctgta gctcgggaaa tcatcaccga
      721 tgcaggttat cccgagttta aacacgccac aggtcaccac ctgggcaggc tagcccacga
      781 tggcgcaggc accattggcc ctctttggga aagatatggc gatacaccca atttccaatt
      841 ggaagctggt caggtataca ccattgaacc tagtttatac cttccagggt atggacatat
      901 gggtattgaa gaggacgttc tggtcagtga aaatggggct gagtatctaa cagtccctca
      961 gcgtgagtta atcctcaagt aatcttttta tcgttagcaa tctcctgagg atttttgata
     1021 tcctcgttta caataatatc gcctaacttt attccaggag gcggatatgt gtgaattttg
     1081 taccgaacac ggcgagggca aagcttggta ccttacgatg aaaaactata gccaggaatt
     1141 gctttcgcaa aatggcagaa tcgaacatat tcgcgcattt ttcaaagatt ttgaaatccg
     1201 cacagcgcaa tccctctcga tgctcgacca gattcaagcc cttccttttg tgcccgatat
     1261 tgtaagccgg gttgtcacat cacgccaaaa aaaggcccac tttggtcaag tcgtaccgat
     1321 cgaagatgtg gatcgcttac tggacgatat atcatccatt gttcgaattc cttgtgtctg
     1381 ccgcagcttg acgacaggca ggcaggaaac gcgctagtgc tacggattag gcattgatcc
     1441 cacaggcttg atcggtgcat atcctgatta tggcgaaaat ctggagtggc taccgcgcga
     1501 ggaagctcgc agcgcaatcc acaaattgga tcagcaaggg ctagttcact ccgtttggac
     1561 ctttgataca ccctttatcg gaggcttgtg taattgtgat caagactgta ttgcctatag
     1621 attgcagatt ggcacaggca tggtgcaagt atttttcccg gctgagcacg tggcatccat
     1681 cgattgggat gattgcaccg gctgtaagct ttgccgtggg tattgttcct tcggggcaat
     1741 acgctacacc tctttacacg acaaatgcct gatcgatccc aatttgtgct atggatgcgg
     1801 tgtatgcagg gcaacctgca agaaggacgc tatccatctt gagcaacgaa agcggacctt
     1861 ccggtggcaa cggaaaatat cccaaccagg acagcaccga gttctggtaa acggctgcca
     1921 gaatgcccgt caatgtcgag catgtatccg ggtttgccca tctcaggtat tcgtcattgc
     1981 tccgcaagag ggacgttctg aaggacagcg cgctacggat tgggttgcca gggctgtatt
     2041 acccagccgc tgtacagatt gccgtgagtg tataacggcc tgccccgcaa acgccattgt
     2101 tgtcaattaa aaaggaatat tatggaatac gttcgtcttg gaaggactgg acttaaagtt
     2161 agtcgaatat gcttgggtac ca
//
LOCUS       DBNHOH010000553         4316 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_994951, whole genome shotgun sequence.
ACCESSION   DBNHOH010000553 DBNHOH010000000
VERSION     DBNHOH010000553.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_994951"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="AC16V7_09600"
     CDS             complement(<1..1161)
                     /locus_tag="AC16V7_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6257415.1"
                     /translation="MAATTLRREIEFVSPTRQMVMGILFVIIGAGIWFFFSQGIQPDV
                     QTKFSLTPGGSTAVLPDWIVPTAMTSNLLSGLSILLGLFQIVRKGGFRRYTNWVLGGV
                     AAMFIFAFLTWAAAGKSLNLAGMINTTLIKAVPLTLGAMSGILCERSGVVNIAIEGMM
                     LMGAMVGALVASIADNLWIGLLAAVASAALLGLVHAVLSIKYKTDQIISGTAINIFST
                     GMTSYISAKFMQVYQTELNNPGVFLPIEIPLLSKIPILGTILFRQNIFFYAMFVFLVL
                     LSFGLFKTRWGLRLRSVGEHPRAADTLGINVFRTRYMAVLLGSMVAGFGGAYFTIGSV
                     GGFDEVMTAGRGFISLAAMLFGNYTPFGSFGAGMLFGFADSLASKLSILGVKI"
     gene            complement(1165..2460)
                     /locus_tag="AC16V7_09605"
     CDS             complement(1165..2460)
                     /locus_tag="AC16V7_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6257416.1"
                     /translation="MSKAQPQSPEGPPKKDAGKIFLEELIGMRPHPGSRWRRILQSIL
                     VPALAIFTGLVLGALVILFTSPEFFAAFKVSLWEGLKTGWKVVSITYGALFVGAFGSP
                     SAIFSALLSGETAAIREAFYPFLESLVASTPYIFGGLSVALGFRVGLFNIGGEGQLFL
                     GAIFATYVGYSLTGLPVFLHMPIAFLAGALGGAVWGFIPGILKAKTGAHEVINTIMLN
                     YVAFRLSEWLLNGPMQRPDSFNPVSPFIQESAKLPRFFAPPIRFHMGFFIALLVAWLV
                     YWFLWKTKWGFSLRMVGSNPHAARYAGASVVMGTVMGMTLAGALAGLAGANEVLGLNY
                     YLAMAFSSGYGFDSIAIALLGNTQPLGVVLSALLFGALRNGATLMQLKGGIPIDIVSI
                     VQAMVLVFVAAPAIIRSVYRLRAPAPGEEAGLATSWGGD"
     gene            complement(2453..4009)
                     /locus_tag="AC16V7_09610"
     CDS             complement(2453..4009)
                     /locus_tag="AC16V7_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6257417.1"
                     /translation="MATVLELKGITKRFPGVLANDHIDLTLEEGSIHALLGENGAGKT
                     TLMNILYGLYQPDEGEIFVRGKPIVVHSPSDAIAAGVGMVHQHFMLVPVFTVTENVML
                     GEEALRYGDVLDRKSAAKKIRQISEQYSLEVDPDAYVEDLPVGVQQRVEIIKLLYREA
                     EILILDEPSAVLTPQEVEELFKIMRSLVAQGKSIIFITHKLKEVLEIADQITVLRRGK
                     VIGTTTPADADQHVLAEMMVGREVQLVVDKESIKLGDVVLDVQDLVVLNQRHQMAVND
                     VSFKVSAGEVLGIAGVQGNGQTELIEALTGLRAVQSGRIQIDGTEVTHATPRKITEQG
                     VAHVPEDRQRDGLVLAFPVSDNLMLNTYYLPPFSKGVVLQESEIVRSADKRIQEFDIR
                     TPGALTSTSSLSGGNQQKVIVAREFSRPIKLLIAAQPTRGLDVGSIEYIHNRLIQKRD
                     EGCAILLMSTELDEILGLSDRIAVMYRGRIVETLKASEATKEGLGLLMAGMRPVSQAA
                     INSIGGADNV"
BASE COUNT         1100 a         1249 c         1108 g          859 t
ORIGIN      
        1 gatcttgacc cccaagatgg agagcttgga ggctagcgag tcggcaaagc caaataacat
       61 cccagccccg aaagagccaa agggggtgta gttgccaaag agcatcgccg ccagagagat
      121 aaaaccgcgc ccagcggtca tgacctcatc gaagccgccc accgatccaa ttgtgaagta
      181 ggcgccgcca aatccagcta ccatggatcc taagagcaca gccatgtagc gcgtgcggaa
      241 gacattgatg cccagcgtgt cagcagcgcg gggatgttca cctacactgc gcaggcgtaa
      301 cccccagcga gttttgaaaa gcccaaagct aagcagcacc aagaatacaa acatagcata
      361 gaagaagatg ttctgccgaa acaggatggt gcccaggatg ggaatttttg acagcagcgg
      421 gatctcgata ggcaaaaata cacccgggtt attcaattct gtctggtaga cctgcatgaa
      481 cttggcggaa atataggacg tcataccagt cgaaaagatg ttgatggccg taccggagat
      541 aatctggtca gttttgtatt tgatggacag cacagcgtgc accaggccga gcaaagccgc
      601 cgaggcaacc gccgccagca aaccgatcca taggttgtct gcaatgctgg cgaccagagc
      661 gccaaccatg gcacccatca acatcatgcc ttcgatggcg atgttcacca ccccggagcg
      721 ttcgcataaa atcccggaca tcgcgcccag ggttagcggg acagctttaa tcagggtggt
      781 attgatcata ccagccaggt tgagcgattt gcccgccgcc gcccaggtca ggaaagcgaa
      841 gatgaacatg gctgccaccc cgcctaaaac ccagttggtg taacggcgaa aaccgccttt
      901 ccggacaatt tggaataacc ccagcaagat agacagcccc gatagcaagt tcgacgtcat
      961 agcggtcggc acgatccaat ccgggagcac agcggttgag cctcctgggg tcaggctgaa
     1021 cttggtctgg acatccggct ggataccctg gctgaaaaag aaccagatac ccgcgccgat
     1081 gatgacgaag agaataccca tcaccatctg gcgtgtagga gagacgaact cgatctcgcg
     1141 tcgtaatgtc gtagctgcca tggatcaatc ccctccccag gaagtcgcca gtcccgcttc
     1201 ctccccaggt gccggggcac gcaagcgata gactgagcga atgatggcag gcgcagccac
     1261 gaataccagc accatcgcct gcacaatcga gacaatatcg atggggatac cccccttgag
     1321 ttgcatcagg gtcgccccat tacgcaaggc accgaacagc aaagccgaga gcaccacacc
     1381 cagtggctgc gtattgccca gcagagcaat agcaatgctg tcaaaaccat aaccggatga
     1441 gaatgccatg gccagataat aatttaaacc caagacttca tttgcgccgg ccaagcctgc
     1501 cagcgccccg gccagggtca tgcccatcac cgtgcccatg accacactcg cccctgcgta
     1561 gcgggcagca tggggatttg accccaccat gcgcaaggag aagccccact tggtcttcca
     1621 caaaaaccag taaaccagcc aggccaccag caaggcgatg aaaaatccca tgtgaaaacg
     1681 gatgggtggg gcgaagaagc gcggcagctt ggcactttcc tggataaaag gactaaccgg
     1741 gttgaatgaa tcagggcgct gcatcgggcc attcaacagc cattcgctca agcggaaagc
     1801 gacatagttg agcatgatag tgttgatcac ctcatgtgcg ccggtcttgg ccttcaaaat
     1861 acctggtata aaaccccaca ccgccccacc cagcgcgcca gccaggaagg cgatcggcat
     1921 gtgcaagaag accggtagac ctgtcagcga gtagcctaca taagtggcaa agattgcccc
     1981 caaaaagagc tgcccctcac cgccaatgtt gaacaagccc acgcgaaatc ctaacgccac
     2041 cgacaggcca ccgaagatgt agggagtcga cgcaaccagg ctttcgagga agggatagaa
     2101 ggcctcacgg atggcagccg tctcaccgct gagcaaagcg gagaagatcg cactcgggct
     2161 gccgaaggcg cccacaaaga gagcgccgta ggtaatgctg acgaccttcc agccggtttt
     2221 caacccctcc caaagagaga ccttgaaagc tgcaaagaat tcagggctgg tgaacaagat
     2281 caccagcgct cccagcacca gcccggtaaa gattgccagt gctgggacta aaatactttg
     2341 cagtatgcgc cgccagcgcg agccgggatg agggcgcata ccaatcagct cttccaagaa
     2401 aatcttgccg gcgtccttct ttggcgggcc ttctggcgat tgaggttgtg ctttagacat
     2461 tgtcagcacc tcctatagaa ttaatcgccg cctgggacac aggtctcatc ccagccatta
     2521 ggagacctaa accttctttc gtggcttccg aggccttcaa agtttccaca atcctgccgc
     2581 gatacatcac ggcaatccgg tctgaaagtc ccaggatctc gtccaactcc gtcgacatca
     2641 gcaggatggc gcacccctca tcccgctttt gaatcaggcg gttatggata tattcaatcg
     2701 aaccgacatc caggccgcgc gtcggctggg cggcaatcaa cagcttgatg ggccgggaaa
     2761 actcgcgcgc aacgatcact ttttgctggt tgccacccga aagcgagctg gttgatgtca
     2821 acgcgccggg tgtgcggatg tcgaactcct ggatcctttt atctgcagac ctgacaatct
     2881 cactctcctg cagtacaacc cccttagaaa atggaggcag gtaataggta ttgagcataa
     2941 gattatctga tactggaaat gccagcacca aaccatccct ttgccggtcc tccggaacgt
     3001 gcgccactcc ttgctcggtg attttgcggg gtgtggcgtg ggtaacttct gttccatcaa
     3061 tctggatgcg accggattgc accgccctca agccggtcaa cgcttcgatc agctcagttt
     3121 ggccattacc ttgcacgcct gcgatcccca acacctcccc tgcactcacc ttgaaggata
     3181 catcattcac agccatctgg tggcgctggt tcagcacgac caggtcttga acatccagaa
     3241 ctacatcccc gagtttgata gattctttat cgaccaccag ctgcacctcg cggccgacca
     3301 tcatctcagc cagcacatgt tggtccgcat ccgcaggtgt ggtcgtgcca atcactttcc
     3361 cacgccgcag gacggtgatt tggtcggcaa tctccaacac ttctttcaac ttgtgggtga
     3421 taaaaataat cgacttaccc tgggctacaa gagagcgcat gatcttgaaa agctcttcaa
     3481 cttcctgtgg ggtgagcact gcgctgggct cgtcaaggat gaggatctca gcttctcggt
     3541 agagcagctt gataatttcc acccgttgct gtacacctac aggcaagtct tcaacgtagg
     3601 cgtcggggtc tacctccagg ctgtattgct cggaaatctg gcggattttc ttggcagcag
     3661 acttgcggtc cagcacatct ccataacgca gcgcttcctc ccccagcatc acattttcgg
     3721 tgacggtaaa cacggggacg agcataaagt gctggtgaac cataccaaca ccagctgcaa
     3781 tagcatcgct gggagaatgg actacaatag gcttaccacg cacaaagatt tcaccctcat
     3841 cgggctggta gagcccgtaa agaatgttca ttaatgtggt cttgcctgcc ccattctccc
     3901 ccaacagggc gtggatactc ccctcttcga gcgtcaggtc gatatgatcg tttgcaagca
     3961 cgcccggaaa acgcttggtg atccctttga gttcaagaac ggttgccatg atgacctttg
     4021 gacctccggc agagcgatct caatgtcttt attagattga aaagacagcc aataaaggat
     4081 ttgcacaaac tcctggttaa tttaaacttg gcttaagtca ttttaccata aaggaaagag
     4141 ccggtctgta accggctcga aaaaagcaag ggggttggcc ccccccaacc ccgtatgttg
     4201 tcttttgtag caaaacctat ggcgatgtct ggatagttcc agcgatgata tccgccttga
     4261 cctgatcaag ctctgccttg agctcggcag gcaccagcga gtccgcatcg tggaac
//
LOCUS       DBNHOH010000554         2798 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_289134, whole genome shotgun sequence.
ACCESSION   DBNHOH010000554 DBNHOH010000000
VERSION     DBNHOH010000554.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_289134"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /gene="coaE"
                     /locus_tag="AC16V7_09615"
     CDS             <1..1116
                     /gene="coaE"
                     /locus_tag="AC16V7_09615"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR6257418.1"
                     /translation="KYVIGLTGNIATGKSVVRRMLEHLGAYGIDADALGHRAIAKGAP
                     GYQPVVETFGKWVLGSDGQVDRAKLARIVFADAEALARLESIIHPLVTQAIDVLIRRS
                     TQRVIVIEAIKLLESGLQLMCDSLWVTHAMQEIQLVRLMKKRGMSEAVARQRIASQPP
                     QDEKVAAANYVIRNDGSFEDTWKQVVAAWQELFPAAETGSLRSVKAAEGELAVQRARP
                     RQASEIASLITRLSGGKRKLQRDDIMEAFGEKAYLLLSLGENPVGLVGWQVENLVART
                     DEVYLDPTIPYNQAMQALTEEVERASRELQCEISLVFLPPELIQRNEVWSSLGYEERN
                     IHSLQVRAWEEAAQESMPPGSKMLFKQLRKDRVLRPV"
     gene            1128..2012
                     /gene="cdaA"
                     /locus_tag="AC16V7_09620"
     CDS             1128..2012
                     /gene="cdaA"
                     /locus_tag="AC16V7_09620"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HIR6257419.1"
                     /translation="MPEFINRLLTNLYFIYERLDWVNAVDLLLVTMVFFAILLLLRDT
                     QAMVLLRGVLLLIVLISLLTSLELLPAFSWLVRSTLPALLLAIPVIFAPEIRRTLERL
                     GRAGAIIPGGKANSASQDAITSVVSAAARLSERKHGALIVMQRTDSLDEFVKTGVSLQ
                     ANVTPELLLQIFYPNTPLHDGAVIITGTDLVAASCVMPLSSSGVLAPTPERQMGLRHR
                     AALGISEVTDAVAVVVSEETGYISAVHGGRMIRRLDSERLENILRAFYRPMERRKALG
                     VILSGLFSRRSTSTREEN"
     gene            2013..>2798
                     /locus_tag="AC16V7_09625"
     CDS             2013..>2798
                     /locus_tag="AC16V7_09625"
                     /inference="COORDINATES: protein motif:HMM:NF019561.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HIR6257420.1"
                     /translation="MWRWLGRNLSTLLLAFLLALVVWVSAVISTDPTVEADFPRPIEL
                     EVLGQDPAFVQVERIPSTVQLTLNAPQSIWANLNQDTVRAWIDLSGIEGGPHEIPVRF
                     QVDPSPVQVVKIDPPVVKVNLEPLVTHTYPITVNVTGDPALGYRRGAVQVTPSDVTVS
                     GPSSLAEQVVDVRVSLDVSNANDTVRNNITVQPVDQNGKPVAGLSVTPSTVELIQVVS
                     LLGGYRNVVVKVTTTGQVAQGYWLTNVSVTPPNVTVFSTNPRLV"
BASE COUNT          610 a          709 c          802 g          677 t
ORIGIN      
        1 aaatatgtga tagggttgac ggggaacatt gctacgggta aaagcgtggt gcgcaggatg
       61 ctggagcatc ttggcgcgta tggcatcgac gctgatgctc tagggcaccg ggctatcgca
      121 aagggcgcgc caggctacca accagtggtg gaaacatttg ggaaatgggt attgggctcc
      181 gatgggcagg ttgaccgggc taaactggcg cgcattgtat ttgccgatgc ggaagccctg
      241 gcacgcctgg agagcataat ccatcctttg gtcacccaag ccatcgatgt gctcatccgg
      301 cgctctaccc aaagggtcat tgtcatcgag gcgatcaagc tgctcgaatc gggcctgcag
      361 ttaatgtgcg actcgctgtg ggtcacgcat gccatgcagg aaattcagct ggtgcgcctg
      421 atgaagaaac gtggcatgag cgaagcggtt gcccgccaac gcatcgcatc ccagccaccc
      481 caagatgaaa aggttgccgc agctaactat gtcatccgca atgatggctc ctttgaggat
      541 acctggaagc aggttgtggc agcttggcaa gaacttttcc cagccgccga aactggatct
      601 ttgcgctcgg ttaaggcagc cgaaggtgag ctagcggtac aacgcgcacg accacggcag
      661 gctagtgaaa ttgcctctct gatcacccgc cttagcggag gcaagcgcaa attgcagcgt
      721 gacgacatca tggaggcatt tggagagaag gcttatttgc tccttagctt gggagagaat
      781 cctgtggggc tggtagggtg gcaggtagaa aatcttgtgg cgcgcactga tgaggtctat
      841 ctcgacccca ccattccata taaccaggcc atgcaggcat tgactgaaga agtggagcgc
      901 gcctcccggg agctgcaatg cgaaatttcc ctggtatttc tgccaccgga attaatacag
      961 cggaatgaag tgtggagctc cctcggttac gaagagcgaa acatccacag tttgcaggtg
     1021 cgagcttggg aggaggcggc gcaagaatca atgccacctg gatcaaaaat gttatttaaa
     1081 caattgcgga aagatcgggt cttgcgcccg gtttagttgt tctggctgtg cctgaattca
     1141 tcaaccgcct acttacgaac ttatatttta tctacgagcg tttggactgg gtgaacgcag
     1201 ttgacctatt gctggtcacc atggtgtttt ttgccatact gctcctgctg cgcgataccc
     1261 aggctatggt gttactgcgg ggtgtcttgc ttctgattgt gctgatcagc ctcctgacca
     1321 gcctggaact gctgcctgct ttctcatggc tggtgcgcag cacactgcca gcacttcttc
     1381 tagcgattcc cgtcatcttc gcacctgaaa tccgccgcac actggagcga ttaggcagag
     1441 ctggcgcgat cattccgggt gggaaggcaa attcggcctc tcaggatgcc atcacctcgg
     1501 tggttagcgc agctgcgcgc ctctccgagc gcaaacatgg ggcattgatt gtgatgcagc
     1561 gcaccgacag cctggatgaa ttcgtgaaaa ccggagtgag cttgcaagca aatgttaccc
     1621 ctgagttgtt gttgcaaatt ttctacccca acactccctt gcacgatggc gcggtgatca
     1681 tcactggcac agatttggta gccgcatcct gtgtgatgcc gctctcttcc agcggcgtgc
     1741 ttgcgccgac ccctgagcgc cagatgggcc tgcgccatcg tgctgcactg ggtatctccg
     1801 aggttaccga tgcggtggct gtggtcgtct cagaggagac cggatatatc tctgcagtgc
     1861 atggtggtcg catgattcgc aggcttgact cagagcgtct cgagaacatc ttgcgggcct
     1921 tctaccgtcc tatggaaaga cgcaaggctt tgggtgtcat attgtctgga ttattttctc
     1981 gtcgatctac ctcgacccgg gaggagaact agatgtggcg ttggctggga aggaacctat
     2041 ctaccctgct gctggcattt ttattggcgc tggtggtttg ggtctctgcg gttatctcaa
     2101 ccgatccgac cgtagaggct gactttccgc gccccatcga gctagaggtt ttaggtcagg
     2161 acccggcttt tgtgcaggtt gagaggattc catccactgt ccaactgaca ctaaatgccc
     2221 ctcaatccat atgggcaaat ttaaatcagg atactgtacg agcctggatt gacctttcgg
     2281 gtattgaggg tgggccacat gagattccgg tcaggttcca ggtggatcca tctccagtac
     2341 aagtggtcaa gattgatccg ccagtagtca aggttaatct ggagccgctt gtcacacaca
     2401 cgtatcctat caccgtcaat gtgactggag atccggcctt gggctatcgc aggggagcag
     2461 tccaggttac tccctctgat gtgactgtat ctggtccgag ctctctggca gaacaagtgg
     2521 tggatgtaag ggtttcattg gacgtaagca atgcaaatga taccgtcaga aataatatca
     2581 ctgtacaacc ggttgaccaa aatggcaaac ctgttgcggg cttaagtgta actccgtcga
     2641 cagttgagct gattcaggtg gtgagtttat taggcggtta ccgcaatgtg gtcgtgaaag
     2701 tgaccacgac gggtcaagtg gctcagggat actggttgac caatgtttca gtcacgccac
     2761 ccaatgtcac tgtcttctcc accaatccta ggctggta
//
LOCUS       DBNHOH010000555         7287 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1121946, whole genome shotgun sequence.
ACCESSION   DBNHOH010000555 DBNHOH010000000
VERSION     DBNHOH010000555.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7287)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7287)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7287
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1121946"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..2551)
                     /locus_tag="AC16V7_09630"
     CDS             complement(23..2551)
                     /locus_tag="AC16V7_09630"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012448869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140358 - P-type transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR6257421.1"
                     /translation="MDQSIEFTMPVSGMTCASCAANIENALNGVEGVAQASVNLATER
                     ASLRLSKPEVKTIDLIQAVRDVGYDVPSETLTLPIGGMTCASCVSTVEGALKGVPGVV
                     DATVNLATEKATVTFVPTLATIKDFKVAVAEVGYQVLDIKEGGGQVAEDESERKMQES
                     RFRMWVAWSFTAPIILWMFLEMFFGIMWPNESIFNLGMVVLGLPVLFWVGRHTYRSGF
                     KAGIRGKPNMDTLIALGTGVSLLTGPASFFFPVANYAGVAAMIMAFHLTGRYVEETAK
                     GRASQAIRKLLELGAKTAWVLKDGKEVEVPIEDVDVGDIMIVRPGDKIPTDGVVLQGD
                     SAVDESMATGESMPVTKRQGDEVIGATINQEGLLKVKATRVGKDTFLSQVIKLVEECQ
                     GSKVPIQEFADKVTGIFVPIVLVIAILTFAAWILISAPMQALVNTGSFLPWVNPNLGV
                     ITLAIVSTVAVLVIACPCALGLATPTALMVGSGKGAEHGILIRTGEAVQTIKDVRIIA
                     FDKTGTITKGRPEVTDILPAEGFHQDEVLRLAASVEVGSEHPLGRCIVEKSRVLELIL
                     EEPQAFLAIRGKGVSATLDSGSVLVGSRRLMLEHGIDPAPLEKTLQQLENEAKTAMLV
                     AAGGRLAGVIAVADTLKEDSIDAIRELHHMGLETAMITGDNRRTAEAIARRVGIDHVL
                     AEVLPDGKVAEVQKLQDRFGLVAFVGDGINDAPALTQANVGIAIGTGTDIAIEASDIT
                     LVRGELTGIVEAINLSNATFQKIKQNLFWAFFYNVAMIPLAIIGWMHPVLAEIAMATS
                     SITVVSNANRLRSVDIRPSYLRGEKVSKRVQESSYSSKGMVVKA"
     gene            complement(2575..2937)
                     /locus_tag="AC16V7_09635"
     CDS             complement(2575..2937)
                     /locus_tag="AC16V7_09635"
                     /inference="COORDINATES: protein motif:HMM:NF014624.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HIR6257422.1"
                     /translation="MTDNRKRILWRLRTVQGHLRAIGDLFEAGETCEEILIQLKAVRG
                     AIDAIAAQLFYDQVERSKQVICEEPSLNRRSEELERLVKLYSAWKQSSFINYNTVSKG
                     ACSRAAAFPTKLQFFEDI"
     gene            complement(2927..3310)
                     /locus_tag="AC16V7_09640"
     CDS             complement(2927..3310)
                     /locus_tag="AC16V7_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257423.1"
                     /translation="MDEENPLITLHEDWATAIDHVEQALASAGFEVIPSFDLQVAQAE
                     AGNFICPIHSNGRCDGQMIELLVYDRDNPPLTLEVLGHNGLVRFAIVDAPQRQSNPLL
                     RAVILQALREMSISKQYPGVGNHDG"
     gene            complement(3369..3608)
                     /locus_tag="AC16V7_09645"
     CDS             complement(3369..3608)
                     /locus_tag="AC16V7_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257424.1"
                     /translation="MDKQRIYQIRVKGQLGEKQWDWLEDMETSVSQDEGFGTITAITG
                     LVEDQALLHGILYRLYSVGYVLISVNCLDEGGRKS"
     gene            complement(4077..6878)
                     /locus_tag="AC16V7_09650"
     CDS             complement(4077..6878)
                     /locus_tag="AC16V7_09650"
                     /inference="COORDINATES: protein motif:HMM:NF037660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR6257425.1"
                     /translation="MTDIILETKIPPPKKRSKILPRPRLIERLDKSLNRNLTLVSAPA
                     GYGKTTLISSWINNLQEPIAWYSLDAQDNDLFVFLSYLITALQSASGDFEKPIDKFLE
                     IVSQPDRKPNHKKVFAQLINEIASFPQRVVLVLDDYHEIKEPSIHDLISDLLTQQPPQ
                     IHIVITTRIDPPLSIARLRVSDEIAEIRAKELRFLGEEIRDFLDQIMSLHLPEPQIAA
                     LESKTEGWIAGLLLAAHSLQYEPESSKFVEAFAGTDRQITDYLIEEVLARLPQEIHEF
                     LLYTSVLDRLTASLCQRLVYGEGTAEQSQEILEWLERNNLFTFPLDNRRFWYRYHLLF
                     AEFLRYRLALVSPEKVFDLHLKASEWMEANGFVTEAINHALVGENNRRALDLIENHGL
                     AAISKAEVRKVQRWFENLSKEMIRSRSSLSILYAWTLLFTNYSHPPKEIDEWIQAAEE
                     ALSMQEGEPDKAKTDKEHKVREHIYAIRPILAFSRHEDPQAVIESGKRALDFIDESNS
                     WLRSILLHSIGAAHIIIGDIESTRRFDLDAQHHAKISGMDYMGIGLYYDMVFIALRQG
                     RLREAESVCREWLDYAHRQGKMESATAGFLYALLGKILVERNDLDSAQTALAKGMDLL
                     PLSGEDELRDLCLVDLCRLYQARGEWSSSERALSENSIDSAGERSAWTESFLSALKAL
                     LWLREAEYTQSRLRLVFQWLEKYAPDLGADTEYPILFPTSEGDYIVQMITARIHIAYA
                     RTLPPTYREKSIQSVLSFLNGQHQIAQKRGWGERVMELEIIRALALETIGELDGAFDA
                     LSRALTIGESEGYIRIFVDEGHVMAKLLYEAASREIYPEYVGKLLATYPKEKPKLSLA
                     MGDEDEEFDQVEPLSERELEVLDLIAQGLSNKEISQKLYISLNTVKGHTSNIYGKLVV
                     KNRAQAVIRARRIGLLQGE"
     gene            complement(7047..>7287)
                     /locus_tag="AC16V7_09655"
     CDS             complement(7047..>7287)
                     /locus_tag="AC16V7_09655"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HIR6257426.1"
                     /translation="GFSLPGVPWTSFFVPETGVALHGKFWHKNFGVQMSNGRVIMRND
                     NAKWVFRRSAAVWEIPVQDRFAWDRRGYGTCVDVH"
BASE COUNT         1790 a         1866 c         1733 g         1898 t
ORIGIN      
        1 gttgcggtca tatcctggaa tcttatgcct tgacgaccat tccctttgag gaatacgaag
       61 actcctgaac tcgtttggaa actttctcac cacgcaagta gctggggcgg atgtcgacgc
      121 tgcgcaaacg attggcgttg ctgaccacgg tgatggaact cgtcgccata gcgatctccg
      181 ctagcaccgg gtgcatccag ccgatgattg ccagcgggat catggccacg ttgtagaaga
      241 atgcccaaaa caggttttgc ttgatctttt ggaaggtagc attactgagg ttgatagcct
      301 ctacgatgcc ggttagctca cctcggacca gggtgatatc actggcttcg atggcgatgt
      361 cggttcctgt tccgatggcg atgccgacat tggcttgagt cagggcgggg gcgtcattga
      421 tgccatcacc tacaaacgcc accaaaccaa aacgatcctg cagcttttga acctccgcca
      481 cttttccatc aggcagaacc tcagctagca cgtgatcaat gccgaccctg cgtgcaattg
      541 cttcggcagt acgtcgattg tctccagtga tcatggcggt ctcaagcccc atgtgatgca
      601 actctcgaat tgcatcaata gagtcttcct tgagcgtgtc tgccacggca attaccccgg
      661 cgagcctgcc cccggcagca acgagcatag cggtcttggc ttcattctcc agctgttgca
      721 gggttttctc taaaggtgct ggatcgattc cgtgctcgag catcagtctt cgtgatccca
      781 caagaacaga gccactatcg agcgtagcac ttacgccttt cccacgtatg gccaaaaagg
      841 cctgtggttc ttctagaata agctcaagga ctcgggattt ctcaactatg catctcccca
      901 ggggatgctc actcccaacc tcgaccgaag cagccagacg gaggacctca tcttgatgaa
      961 aaccttcggc tggaaggata tccgtgacct ccggacgtcc cttggtgatg gtaccggtct
     1021 tgtcaaaggc aatgatgcgc acgtccttga tggtctggac cgcttcaccg gtgcgaatga
     1081 ggatgccgtg ctcggcgccc ttgccgctac ccaccatcag ggcggtaggg gtggccaacc
     1141 cgagagcgca ggggcaggca ataaccagca cagctacggt ggagacaatg gccagggtga
     1201 tcacccccag attgggattg acccagggca agaaggatcc agtattaacc aaagcttgca
     1261 tgggtgcaga aattaagatc caggcagcga aggtcaggat cgcaattacg agcacgatcg
     1321 gtacgaagat gccggttact ttatcggcaa attcttggat cgggactttg gaaccctggc
     1381 attcttccac cagcttgata acctgggaga ggaaggtgtc tttgccaact cgagtggctt
     1441 ttaccttgag cagcccttcc tggttaatgg tagcgccaat tacttcatcc ccctggcgtt
     1501 tggttacagg catggactct ccagtagcca tggactcatc aaccgcacta tcgccttgta
     1561 agactactcc atcggtgggg attttgtccc cagggcgaac gatcatgatg tccccgacgt
     1621 caacatcctc gatagggact tcaacttcct ttccgtcttt aagaacccaa gcggttttcg
     1681 ctcccaattc caggagcttg cggatggcct gagaagcgcg tcccttggcg gtttcctcca
     1741 catagcgacc ggtcaggtga aatgccatga tcatggctgc cacacccgca tagttggcga
     1801 cggggaagaa gaaggaagct ggaccggtga gaagggagac gcccgtgcct agcgcaatga
     1861 gcgtatccat gtttggtttg ccccgtattc cagccttaaa tcctgaacga taggtatgtc
     1921 tgccaaccca gaacaagacc gggagaccca gtaccaccat ccctagatta aatatggact
     1981 cattgggcca catgattcca aagaacatct ccagaaacat ccataaaatg atgggtgcgg
     2041 tgaaactcca ggcgacccac atcctgaagc gagattcctg cattttgcgc tcagactcgt
     2101 cttcggcgac ttgaccaccg ccttccttga tgtccaggac ttgatatcca acttcagcaa
     2161 ccgcaacttt gaaatccttg atcgttgcca gggtggggac gaaagttact gtagcttttt
     2221 ctgtagcgag gttgacggta gcatcaacta ccccgggaac acccttgaga gcgccttcca
     2281 cagtggatac acaggaggca caggtcatgc cgcctatggg caaagtgagg gtctcactgg
     2341 gtacgtcgta accgacatct cttacagcct ggatcaagtc gatcgtcttg acctcaggct
     2401 ttgacaggcg aagactggcg cgctcggtag ccaggttaac gcttgcctgc gccaccccct
     2461 ctacgccatt caatgcgttt tcaatattgg ctgcgcatga ggcgcaggtc ataccggaaa
     2521 cgggcattgt aaactcgatt gattggtcca tgatttaatc tccttatgtt ttgattagat
     2581 atcttcaaaa aattgcagtt tggtggggaa tgcggcggct ctgctgcatg ctccttttga
     2641 gacggtgttg tagttaataa agctcgattg tttccatgca gaatacagct tcactaagcg
     2701 ttccaattct tctgagcgcc ggttgagaga gggctcctca caaatcactt gcttgcttct
     2761 ttcgacttgg tcataaaaca attgggctgc gatcgcatcg atggcgcctc gtacagcttt
     2821 caactgaatg agtatctcct cgcaggtttc cccggcttcg aagaggtcgc caatcgcacg
     2881 cagatgtccc tggacggtac gcaggcgcca aagaattctc ttccggttat ccgtcatggt
     2941 tcccaactcc ggggtattgc tttgaaatac tcatttccct taatgcctgt aagatcactg
     3001 ctcgcagaag ggggttggat tgcctttgag gcgcatctac tatcgcaaaa cgtaccaggc
     3061 cattatgacc aagaacttcc agggtgagcg gcggattatc ccggtcataa accaggagct
     3121 caatcatctg gccatcgcat cgtccattgc tgtggatggg gcagataaag ttcccagctt
     3181 ctgcctgagc gacctgtaag tcgaaagatg ggataacttc aaagcccgca gaagcaaggg
     3241 cttgctcaac atggtcgatg gcggtagccc agtcctcatg tagtgtaatc aacgggtttt
     3301 cttcgtccat atccatacat ccgttcactt gtcgtgcatt tggtcacagg catcctgata
     3361 ttcacacctt aagacttgcg tccgccctca tccaaacagt tcacggaaat cagcacataa
     3421 cccacactat agagtcggta caggattcca tggaggagcg cctggtcttc gaccaacccc
     3481 gtgatcgcag taatggtgcc gaaaccttca tcctgggata cgcttgtctc catatcctca
     3541 agccaatccc attgtttctc tccaagctgg ccttttactc tgatctgata tatcctttgt
     3601 ttatccatag cagcctcatg gtctgtatag ggctcttgat ttgactcttt ttggatcgcc
     3661 ttagacggat cagtttcagc atccgctgtt tctctgcctc ttgcaaaaag gcatttactc
     3721 tatctttgta ggcttcaatt tctaattggg gtaacatagc ttttgtctct gttgaaattg
     3781 ttttgtgctg aactgactat gggttttctt ttccttcctc agataagtgc aatatacgat
     3841 tatgttttca tgggatattg aggtttagct cccaattctc accgatatct gcactccttg
     3901 gataattatc tacagcatac ataaaagatg gttgttcgtg acgacccata agtgttattg
     3961 aggtattgtt ctatttatcg aatatccttt agcgcacaat gcttataaag aaaaaacggg
     4021 ctgtcatcag ataaaatgac aacccgttcc aattttcaaa atatgccaaa tttatattat
     4081 tccccctgca acaatccaat cctcctggcc cggatcacag cctgagccct attctttaca
     4141 accagctttc cataaatatt gcttgtgtgt cctttgacag tgtttaggga aatatagagt
     4201 ttttgagaaa tttctttgtt tgaaagccct tgtgcgatca agtcaagaac ctcgagctcg
     4261 cgctcgctca acggctcgac ttgatcaaac tcctcgtctt catctcccat tgccaggctt
     4321 agttttggct tctcctttgg ataggtggcc agcagcttgc caacgtactc ggggtagatc
     4381 tcccgggagg cggcctcgta cagcaactta gccatgacat gcccttcatc tacgaagatg
     4441 cggatatagc cttcagattc cccaatagtg agggcgcgcg acagggcatc gaaggctcca
     4501 tccaattcac cgattgtttc taaggctagc gccctgatga tctcaagttc catcacccgt
     4561 tcaccccatc cccgtttttg ggcaatttgg tgttgaccat tcaggaagct taacaccgat
     4621 tgaatggatt tctctcgata ggtagggggc aaggttcgcg cataagcgat atgaattctg
     4681 gcggtaatca tctgcacaat atagtctccc tcagacgtag gaaagagtat tggatattcg
     4741 gtgtccgctc ccagatcagg agcgtatttt tccagccatt ggaagactaa gcgaagcctg
     4801 gattgtgtat attcagcctc cctgagccat agcaaagcct ttaaggcgga caggaaggat
     4861 tcagtccaag ccgatcgttc accagcagaa tcaatggagt tttccgataa ggccctttcc
     4921 gaactggacc attcaccccg ggcttgatac agcctgcata gatccacaag gcacaaatca
     4981 cggagttcat cttccccgct caagggaagc aaatccatcc ctttcgccag cgccgtttgg
     5041 gcggaatcaa ggtcattacg ttctaccaag atttttccaa gcaaagcata aagaaatcca
     5101 gcagtggcgg attccatttt cccctggcgg tgagcataat caagccattc acgacaaacc
     5161 gattccgcct cacgcagcct gccttgtcga agcgcgataa aaaccatgtc gtaatataac
     5221 ccaatcccca tgtagtccat accgctaatc ttcgcatggt gttgcgcatc caagtcaaag
     5281 cgtctggtgg attcaatgtc tcctatgatg atgtgagctg cgccaattga atgcagtaag
     5341 atgcttctca accaggaatt gctttcatca ataaaatcga gggctcgctt tcccgattca
     5401 atcactgcct gaggatcttc atgcctggag aaggccagta tggggcgaat tgcatagata
     5461 tgctctctga ctttgtgctc tttgtcggtc ttggctttat ccggttcacc ttcttgcatc
     5521 gacagtgctt cttcggccgc ctgaatccat tcgtcaattt ccttcggggg atgggaataa
     5581 ttggtgaaca aaagggtcca tgcataaaga atagaaaggg aagaacgaga tcgtatcatc
     5641 tccttggaaa ggttttcgaa ccatctttga acctttcgga cttctgcttt ggatatcgcg
     5701 gccaagccgt gattctcgat caagtctaag gctcgcctat tattctcacc caccagcgca
     5761 tggttgatag cttcagtaac aaatccattc gcttccatcc attcacttgc tttgagatgg
     5821 agatcgaata ctttttcggg agaaactaaa gccaatcggt aacgtaaaaa ctcagcaaac
     5881 aataagtgat atcggtacca gaagcggcgg ttatccaacg ggaacgtaaa cagattgttt
     5941 ctctcaagcc actccaggat ttcctggctt tgttccgctg tgccttcacc atataccaat
     6001 cgttgacaaa gagaggcagt taaacggtca agaaccgagg tataaagaag gaattcatga
     6061 atttcctggg gtaaacgcgc cagaacctct tcaataagat aatctgttat ctgccgatcg
     6121 gtccctgcga acgcttctac gaacttcgat gactccggct cgtattgcag ggaatgggca
     6181 gccagaagca gaccagcaat ccaaccttca gttttcgact ccagagcggc gatttgtggc
     6241 tctggcaaat gcagagacat gatttgatcg agaaaatccc tgatttcctc tcccaagaag
     6301 cgcagctctt tggctcgaat ttcagctatt tcatcgctca cgcgcagcct ggcaatcgat
     6361 aggggcggat cgattctggt tgtgataacg atgtgaatct gtggaggttg ttgtgttaag
     6421 agatcactta tcaaatcgtg gatggaaggt tctttaattt catggtaatc atccaagacc
     6481 aggaccactc tttggggaaa tgatgcgatt tcgttgatta attgagcaaa tacctttttg
     6541 tggtttggtt ttcgatctgg ctgggaaaca atttctagaa atttatcaat cggtttttcg
     6601 aaatccccac tagccgattg caaggctgta ataagataac ttagaaaaac aaaaaggtca
     6661 ttgtcttggg catccaaaga gtaccaggcg ataggttcct gcaaattatt aatccaactc
     6721 gaaatcagag tggtcttccc atatcctgct ggagcagaca cgagggtcaa attcctgttt
     6781 aagctcttat ccagacgctc aatcagacgc ggtcgcggca atatttttga tctttttttg
     6841 ggcgggggaa tttttgtctc gagaataata tcagtcattt gttattctat tgatattgag
     6901 agacaaggag gtccttcttg gaagggagtg caggtattga ggaaaataca aacctactcc
     6961 attagcagtt ccgaacaaga gaaaaagtct cgtaacagag tatatcggat aagaattgtc
     7021 ctgacaagca tcaaatggcg gctgtgtcag tggacatcca cgcaggtacc atagcctcgc
     7081 ctgtcccagg caaaccgatc ctgtaccggg atctcccaaa ccgcagccga ccgccggaag
     7141 acccacttgg cattatcgtt gcgcataatg acgcgcccat tgctcatttg caccccaaag
     7201 tttttgtgcc agaatttgcc atgaagggcg acaccggtct ccgggacgaa aaagctggtc
     7261 caggggactc caggtagcga aaaacca
//
LOCUS       DBNHOH010000556         4443 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_160311, whole genome shotgun sequence.
ACCESSION   DBNHOH010000556 DBNHOH010000000
VERSION     DBNHOH010000556.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_160311"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(310..382)
                     /locus_tag="AC16V7_09660"
     tRNA            complement(310..382)
                     /locus_tag="AC16V7_09660"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:ccc)
     gene            complement(585..1661)
                     /locus_tag="AC16V7_09665"
     CDS             complement(585..1661)
                     /locus_tag="AC16V7_09665"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR6257427.1"
                     /translation="MINIAKPMIGSEEKQAVLEVLDSGMLAQGPRVKAFEEAFADMCG
                     VKYAIATSSGTTSLHTALLAHGIGPGDEVITSSFTFIASANSILFVDARPVFVDIDPR
                     TFNLNPALIEAAITPRTKAIMPVHLYGLSCDMNPIMEIASKYNLLVIEDACQSHGATY
                     KGRAVGSFGAGTFSLYPTKNMTSGEGGMITTNDEAIAEHCRVIRQHGMRRRYYHDELG
                     FNFRMTDLQAAVGLEQLKKLGKFNQARVQNARFLSSHLKGVGVPFVPDGHEHVYHQYT
                     IRVPGGRRDALVEHLNKQGVGCMVYYPVPIHKQTFYVNELGYNMSLTETECAAQEVLS
                     LPVHPGLSNEDLETIVAAVNNFMS"
     gene            complement(1658..2263)
                     /locus_tag="AC16V7_09670"
     CDS             complement(1658..2263)
                     /locus_tag="AC16V7_09670"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011011896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HIR6257428.1"
                     /translation="MGIRIHPTAEVSEKATIGEGSSIWHQAQVRENVKIGMNCIIGKG
                     VYVDAGVSIGDNVKIQNYVSVYHGVTIEDGVFVGPHVCFTNDLRPRAINPDGSLKSAD
                     DWVLGLTLIKRGAALGANSTIRCGTTIGEWAMIGSGSVVTRDIPSNGLAWGNPAQLHG
                     FVCPCGEKLEKTGESGNQIVATCPICGRITEIAKKDWKGII"
     gene            2402..4219
                     /locus_tag="AC16V7_09675"
     CDS             2402..4219
                     /locus_tag="AC16V7_09675"
                     /EC_number="1.2.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF013481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HIR6257429.1"
                     /translation="MSIDLYTGKTGEIEIPQEWERDYIGGASLAARLLYDHLTPDLDP
                     LSARAPLLFLNGPLSGTAGPAVGRFVISARSPATGLWGESNCGGFWGPELRKTGYDGL
                     WITGRAESPVYLWIQNNQVEIREAKHLWGLDTYQTQDTILEELHIGKARIAAIGPAGE
                     ALTPFALILVDHGRVAGRTGLGAVMGSKNLKAIAVQGSGKVPVPNPKMYAPLRSETNR
                     LLKGDTQTKVAHELGTASVADYMDYLGIMPKKYFQTDVFDGATNVSGATIAESILAGT
                     SACHACVIACGRVVRLEDNLKRKGPEYETLMGFGPNLLLDDPSFATRIGELCDRYGMD
                     SISLSNVIGLAFKLYEKGVITDVDTGGIALRWGDANAVEKLVQMVGSRSGFGAFLVEG
                     AQAFARRYGFEDEAVHVNGLEVPYHDPRGGSGMALVYATSPRGACHNQSDYFMVEIGQ
                     AEAEIGIEFLPRQAGLEKALNVARHQNWRTVFNALVMCIFTNIAPKRVVSLINTICEE
                     QWTIVDVLRSGERAWNLKRAINIRLGLRRENDSLPKALLTSLGVGGGTGGYVPPFQEM
                     LEAYYIARGWDRVNGIPLHSKLEELGLEFVARDLDELRI"
     gene            4237..>4443
                     /locus_tag="AC16V7_09680"
     CDS             4237..>4443
                     /locus_tag="AC16V7_09680"
                     /inference="COORDINATES: protein motif:HMM:NF015791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HIR6257430.1"
                     /translation="MNRVKYVSVAEMVAIEKEANASGLTYPLMMENAGRGLAEEVHAA
                     YSHIDERSVLGLVGSGNNGGDTLVA"
BASE COUNT         1125 a          984 c         1174 g         1160 t
ORIGIN      
        1 gctttcagct tcaagaacgc tatacccttc ggcttgtaat atcctgcgca caagcatccg
       61 attctcatta ttatcttcaa tgtatagaac agtagcaaca atatgcatat ctagctgtca
      121 aaaaatgata actctaatag ttaacacgtt tgatttaatg tattgtttca cctgtcaaat
      181 gaaaattgca cttttgtcag attgatgtag ggattttata tgtgtatgga ttgttgattg
      241 attaaatttg catgtcagag atttaagttg agaagcactc tggtacgacc agagtgcttc
      301 tgtaaagaga gcgggtgatg ggattcgaac ccacgaaatg gcagcttggg aagctgctgc
      361 cttaccactt ggcgacaccc gcaatgagga gattatagcc acccgagggg gagatgtcaa
      421 gtaaaaccca cgctaagctg ctttcccgtt gcttatcccc ggttgtatgc agtacgtact
      481 gggggtaaac acaggtgcgc tataaattta tagcgcacct gggagaatcc ggcagatatc
      541 gtaaggtgat taagtcactg tagtatttgc actaaaaatc caactcagga cataaaattg
      601 ttgactgccg caacgatcgt ctctagatct tcattgctaa ggccaggatg cactggcaag
      661 gaaagaacct cttgtgcagc acattcagtc tccgtcaagc tcatgttata acctagctcg
      721 ttgacataga atgtctgttt gtgaatcggc acggggtaat ataccatgca tccgacgccc
      781 tgcttattga ggtgctcaac caaggcatcg cgccgtccac ctgggactcg gattgtatat
      841 tgatgatata catgctcgtg accgtctggg acaaatggta ctccaacacc cttcaggtgg
      901 ctgcttagaa agcgtgcgtt ctggacgcgt gcttggttga atttacccag cttttttagt
      961 tgttctaggc caacagctgc ctgaagatcg gtcattcgga agttgaagcc caactcatcg
     1021 tggtaataac gccgtcgcat gccatgctgg cggataactc ggcaatgttc cgcaatggct
     1081 tcgtcattgg tagtgatcat gccaccttca ccagaagtca tattttttgt ggggtagagc
     1141 gagaaggtgc ctgccccaaa tgatccaact gctcggcctt tgtacgtagc gccatgactc
     1201 tggcaagcat cttcgattac caacaggttg tatttagagg ctatctccat gattgggttc
     1261 atgtcgcaac tcaatccata aaggtgcacc ggcataatgg cttttgtgcg cggagtgatg
     1321 gcagcttcga tcaaagccgg gttgaggttg aaggtgcgcg ggtcgatatc cacaaaaacg
     1381 ggtctggcat ccacaaacag gatgctgttt gctgaggcga taaatgtaaa ggaagaagtt
     1441 attacctcgt ctcccggtcc aattccatga gctaataggg cagtgtgcaa tgaggtagtt
     1501 cctgaggagg tggcaatagc atatttcacg ccacacatgt ctgcgaaggc ctcctcaaaa
     1561 gcttttaccc gcggaccctg cgcaagcata cccgaatcga ggacttcgag tacggcttgt
     1621 ttttcttcag agccaatcat cggtttggca atattgatca tatgattcct ttccaatctt
     1681 tctttgcgat ttcggttatg cgcccgcata tcgggcaagt ggctacgatt tggtttccgg
     1741 attcgccggt cttttctagt ttctctccac atggacagac aaaaccatgc agttgtgcag
     1801 gattgcccca tgctaatccg tttgatggga tatcgcgagt gacaacgctg ccagacccga
     1861 tcattgccca ttctccgatg gtggtgccac aacgaatggt tgaatttgcc cccagggcag
     1921 ccccccgttt gatcagcgtc aatcccaata cccaatcatc tgcagacttc aacgaaccat
     1981 ctgggttgat tgcacgcggg cgcagatcat tggtgaagca aacatgcgga cctacaaata
     2041 cgccatcttc aatagtaacg ccatggtaaa cagaaacata gttttgtatc ttgacattgt
     2101 ccccaatgga cacacctgca tcaacatata cacctttccc gatgatacag ttcatgccaa
     2161 ttttcacgtt ctcgcgcacc tgtgcctgat gccagatgct gctaccttcg ccaatcgtgg
     2221 ctttttccga tacctctgca gtggggtgga tgcgaattcc catgaatact ccctgatgca
     2281 tcaataatta gtgaattcta tgttcactcg tttgaatatt tggtaaactc tcacgtagta
     2341 taacgctaat tcatagccat gttacttata atatcgtcag gtttgaggta tgcagccgat
     2401 tttgagcata gatttataca caggtaaaac gggcgaaata gaaattccac aagaatggga
     2461 gagagattat atcggtggag catctctcgc cgcccgcctg ctatacgatc atttgacacc
     2521 ggatctcgat cccctttccg caagagcgcc tttgcttttc ttaaatgggc cattatcggg
     2581 taccgctggg cctgctgtgg ggcgttttgt aatatctgcc cgctcgcctg ccaccgggtt
     2641 gtggggagaa tcgaactgtg gtgggttttg gggtccggag cttcgaaaaa ccggctatga
     2701 tggcttgtgg ataacagggc gcgctgaatc ccctgtttat ctctggatac agaataatca
     2761 ggtcgagata cgtgaggcca aacatctatg ggggttagat acttaccaga cacaagatac
     2821 aatcctagaa gaattgcata tcggcaaggc gcgcatcgct gcaataggcc cggcaggtga
     2881 ggcgttaacc ccatttgcat tgattctcgt tgatcatgga cgagttgccg gacggacagg
     2941 gttgggggct gtgatgggct cgaagaacct aaaagctata gcagtacaag ggagtgggaa
     3001 ggtgccagta ccaaatccca aaatgtacgc acctctgcgc tcagaaacta accgcttgct
     3061 taagggagac acgcaaacca aggtagcgca tgagctgggc acggcaagtg tggctgatta
     3121 tatggattat ctggggatta tgcccaaaaa atattttcaa accgatgttt ttgatggggc
     3181 gacaaatgtt tctggcgcta ctatcgctga gtcaattctg gccggtacaa gcgcatgcca
     3241 tgcttgtgtc attgcgtgtg ggcgcgtggt acgattagag gataacctaa aacgtaaagg
     3301 accagaatac gaaacgctga tgggttttgg gcccaattta ttgcttgacg acccatcatt
     3361 tgccacgcgc atcggtgagt tgtgtgatcg atatgggatg gatagcatca gtctcagcaa
     3421 tgtcatcgga ctggcattta agttatatga gaaaggtgtg atcacagacg tagacacagg
     3481 tggcatcgct ctgcgttggg gtgacgcaaa tgcagtggaa aaactggtac aaatggtggg
     3541 tagccgatcg ggttttggtg ctttccttgt agagggggcg caagcctttg cccgcagata
     3601 tggctttgaa gacgaggctg tccatgtcaa cggattagag gtgccatacc acgatccgcg
     3661 cggaggatct gggatggctc tggtttatgc tacatcgccg agaggtgcct gtcacaacca
     3721 gtctgattac ttcatggtgg agatcgggca agcagaggca gagattggga ttgagttcct
     3781 gccgcggcag gctggattgg aaaaagcgct gaacgttgct cgacatcaaa attggcgtac
     3841 ggtattcaac gcactggtaa tgtgtatctt taccaacata gccccaaagc gagttgtttc
     3901 tcttataaat acaatctgtg aagaacaatg gacgattgta gatgttctcc gaagtgggga
     3961 gcgtgcctgg aatttgaagc gagccataaa tatcaggctg ggtttgcgac gggagaatga
     4021 cagcctaccc aaggcgttat tgacttcatt aggggttgga ggtggtacgg gcggatatgt
     4081 ccctccattt caagagatgc tggaagctta ttacatagcg cgtggctggg atagggtgaa
     4141 tggaatccct ctgcacagca agttggaaga attgggatta gaatttgtgg ctagagattt
     4201 ggatgagtta cgaatctgag cacttctgag ggaataatga acagggtcaa atatgtcagc
     4261 gtagccgaga tggtggcaat cgagaaagag gcgaacgcca gcgggctaac ctatccattg
     4321 atgatggaga atgccgggcg gggtctggca gaagaagtgc atgctgcgta tagccatata
     4381 gatgaacgga gtgtgctggg tttggtgggg tccggtaata atggcggcga tactctggtt
     4441 gcg
//
LOCUS       DBNHOH010000557         2408 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1058881, whole genome shotgun sequence.
ACCESSION   DBNHOH010000557 DBNHOH010000000
VERSION     DBNHOH010000557.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1058881"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1552
                     /locus_tag="AC16V7_09685"
     CDS             <1..1552
                     /locus_tag="AC16V7_09685"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HIR6257431.1"
                     /translation="AMVFGALVAATDPVSVVSLFRTMGVPKRLQVLLEGESLFNDGTA
                     IVVFDLVVVLAASEASHFNLASSVVEFVRVSGGGLVVGLLLGALISQMISRIDDYLIE
                     TTLTSVLAYGAYLIAEILGVSGVLAVVAAGLINGNIGPRGMSPTTRIVVFNFWEYAGF
                     LANSFIFLLIGLQIHLPVLYQNWQMIVWAIVAILIARAIGVYGLSWFEKEIPPRWRHV
                     LYWGGLRGAISLALALSLPASLPQASPQLQAMAFGVVLFTLLVQGFTMKPLVTQLKLT
                     ERSESQDEYERRHARAVATRHAYEHLSRHHSQGLLSDHSWDIIGPLLQEHNRTLADSV
                     QEVVSSDPQVEQEELDTARREYLSAQRSAINSLRRDGTISDEIFNQLTAEIDGAITNP
                     SAGWPELLSGNISQHTQVNRLMAVIVQEQDVENALSTLNKLGIPTTRLPSTGGFFRRT
                     NAILLIGIEKGQEASVVRALQLSCRKRVEYISAPIEGFPPGLPEPIPITVGGATIFVF
                     EVERFEIF"
     gene            complement(1586..>2408)
                     /locus_tag="AC16V7_09690"
     CDS             complement(1586..>2408)
                     /locus_tag="AC16V7_09690"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR6257432.1"
                     /translation="PVITSAIAAIPIAIVFIISGSTHVDLQAIMGSSVGMTGLMLVGF
                     LPIYLLVWGWLRVFEHRPLWTIGMERKEWLSKYLRGALVGSVMIGAAVGLPAMFGYIQ
                     LQGNSSGVSVATMGALILFMGWVVQGAAEEVLFRGFLLQIIGSRFGVIAGVLVTSILF
                     ALLHIFNASLGVIALINLMLFGVFAAFYTLWEGSLWGVFAVHSFWNWVQGNLFGMEVS
                     GLPVRLEALIDLKEVGPDWFTGGAFGPEGGIAVTLLLLAGIAVLWLLESRKQSES"
BASE COUNT          604 a          699 c          577 g          528 t
ORIGIN      
        1 cgccatggtg tttggtgctc tggtagcggc cacagacccg gtatccgtgg tatccctgtt
       61 tcgcaccatg ggcgtgccta agcgcctgca ggtgcttctt gaaggcgaga gcctgtttaa
      121 tgacggaacc gccatcgtgg tatttgatct ggtagtcgtt ctagccgcat cagaagcttc
      181 ccacttcaac cttgcctcca gcgtggttga gtttgtacgc gtctcaggtg gaggcctcgt
      241 ggttggcttg ttgctgggag cgctaatctc gcaaatgatt agtcgcatag atgattactt
      301 aatcgagaca accctcacca gcgtgctcgc ctatggcgcc tatctgattg cagaaatcct
      361 cggagtgagc ggtgtgctgg cagtcgttgc agccggtttg atcaatggca acatcggccc
      421 gcgtgggatg tcaccgacca cgcgcatcgt ggtattcaac ttctgggaat atgcaggctt
      481 cttggctaac tcattcattt tcttgctgat tgggttacaa atccatttgc ctgtcctgta
      541 ccagaactgg caaatgatcg tttgggccat cgtggctatc ctcatcgccc gagccatcgg
      601 ggtttatggt ctatcctggt tcgagaaaga gataccaccc aggtggcgtc acgtgctcta
      661 ttgggggggc ttgcgcgggg caatttcatt ggctctggcg ctcagcctgc cagcatcatt
      721 gccgcaagcc agccctcagc tacaagccat ggcctttggt gtcgtgcttt tcacccttct
      781 cgtccaaggc ttcaccatga aacctcttgt aacccagctt aagttaaccg agcgcagcga
      841 gtcccaggat gaatatgagc gccgccatgc tcgtgcagtt gctacccggc atgcttatga
      901 gcacttgagc cgccatcact cccagggatt gctgtcagac cattcttggg atatcatcgg
      961 acctctgctg caagaacata accgcacact ggcagattct gtacaagagg tcgtgtcctc
     1021 agatccacaa gtagaacaag aagagcttga taccgcccgc cgggaatatc tgagtgcaca
     1081 gcgcagcgca atcaacagcc tacgacgaga tggcaccatc tcagacgaga ttttcaatca
     1141 actcaccgct gaaatcgacg gggcgatcac taatccatcc gcaggttggc cagagttgct
     1201 cagtgggaat atttctcagc atacacaagt caaccggttg atggctgtca tcgtacaaga
     1261 gcaagatgtc gagaatgcac tgagcacctt gaacaaattg ggcatcccca ccacgcgcct
     1321 ccccagcact ggtggatttt ttaggcgcac aaacgccatc ctattgattg ggatagaaaa
     1381 gggtcaagag gcgagcgtgg tcagggcttt acaattaagt tgccgcaagc gagtagaata
     1441 catctccgcg ccgattgaag gcttcccgcc cggcctgcca gagccaatac ccataactgt
     1501 tggcggcgcg acgatatttg tctttgaggt tgaacgattt gaaatctttt gaataagaat
     1561 acaacccaac gcgaatgccg aaatgtcagg attcgctctg cttgcggctt tcaagcaacc
     1621 ataaaacggc aatccccgcc aacaacaaca gcgtgaccgc gataccccct tctggtccaa
     1681 acgctccccc agtaaaccag tcaggtccta cctcttttag gtcgatcaag gcctccaagc
     1741 gaaccggcag accactcact tccatgccaa aaaggttacc ttgcacccaa ttccagaaac
     1801 tatgcactgc aaaaactccc caaaggctcc cctcccacaa ggtatagaac gctgcaaata
     1861 ccccaaatag catgagatta attaacgcga ttacgccgag actggcattg aaaatatgca
     1921 gtagggcaaa caagatcgag gtaaccagca ccccagctat cacaccaaag cgggagccaa
     1981 taatctgaag cagaaaccca cgaaacagca cctcttcggc cgcgccttga accacccaac
     2041 ccataaatag aataagcgcc cccatcgttg caacgctaac tccagagcta ttcccttgga
     2101 gctgaatgta accgaacatg gcgggcaagc ctaccgcagc tccgatcatc acgctgccca
     2161 ccagcgcgcc acgcaggtat ttactcaacc attcctttct ctccatccct atcgtccaca
     2221 gtggacgatg ttcgaaaacg cgcaaccagc cccaaaccaa gaggtagatc ggcaaaaagc
     2281 ccaccagcat cagccccgtc atcccaacac tcgaacccat gatcgcctgc aaatcgacgt
     2341 gtgtggagcc cgaaataatg aatacgatcg caatcggtat ggcggcgatt gcagatgtaa
     2401 tcactgga
//
LOCUS       DBNHOH010000558         4175 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_930916, whole genome shotgun sequence.
ACCESSION   DBNHOH010000558 DBNHOH010000000
VERSION     DBNHOH010000558.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_930916"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..431)
                     /locus_tag="AC16V7_09695"
     CDS             complement(3..431)
                     /locus_tag="AC16V7_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR6257433.1"
                     /translation="MTFYISPYRRMATLRNMMNRMFEESLDETPNGEREMMLAVDVKA
                     EDEAYDIVALVPGLEPEDINIEIINNTVTLRGEFKSVSDEDGKSLVNELPAGRFRRVI
                     TLPVALDSSKTEASIKNGVLHLRVPKAEAHRPKSIKVVAA"
     gene            complement(674..1408)
                     /locus_tag="AC16V7_09700"
     CDS             complement(674..1408)
                     /locus_tag="AC16V7_09700"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6257434.1"
                     /translation="MLARLYHAHHNAHQEDLPFWLDLARLQGDPILELGCGSGRVLLP
                     LAQAGHFVLGLESDPDMLVVLRENVPPALAERVGTIQADMAAFELGQQFALILLPCNT
                     FSTLTTEARRATLDCVQRHLAPQGLFAVSLPNPTLLRKLLGRSQPEVEESFPHPLDGE
                     PVQVSSSWVRTTGHFVVRWDYDHLLPDGNVERFSMQARHALLSTDDYIAELEAAGLSQ
                     AALYGDFDRSPYSHQAPYLIVTARRK"
     gene            1455..1528
                     /locus_tag="AC16V7_09705"
     tRNA            1455..1528
                     /locus_tag="AC16V7_09705"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1489..1491,aa:Pro,seq:tgg)
     gene            1566..1877
                     /locus_tag="AC16V7_09710"
     CDS             1566..1877
                     /locus_tag="AC16V7_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257435.1"
                     /translation="MARIKFDGVVEAVRYKPSGEIDWVRAYERRGPTFSDYILLDRET
                     LIKRIKAGKRYVSGKRIPYMASTFETSSPIRLVTNSGKEIVVTSDPKATQDRLEGVPT
                     I"
     gene            complement(1978..2721)
                     /locus_tag="AC16V7_09715"
     CDS             complement(1978..2721)
                     /locus_tag="AC16V7_09715"
                     /inference="COORDINATES: protein motif:HMM:NF016300.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HIR6257436.1"
                     /translation="MRKKVFLGIALLLVAALLSAFSSGTEVLAVTQQGPGPETPPRQL
                     NVTGSGKAFVTPDIAYINIGVHTENADAAEAVASNNARSKKVADAVKKFNIDPKDIQT
                     TNFSIFPQQQYDPTGKITGVVYFVDNTVFVTLRDISKVGDVIGAAVGAGANSINSIQF
                     DAADRSKALSEARKSAVADALATAKELASAAGVTLGDIQMINSYGGGAPVPIYDAKGY
                     GGGGAGAPVPVSPGQLVLMVDVNIVFEIK"
     gene            2982..>4175
                     /locus_tag="AC16V7_09720"
     CDS             2982..>4175
                     /locus_tag="AC16V7_09720"
                     /inference="COORDINATES: protein motif:HMM:NF016013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HIR6257437.1"
                     /translation="MSENLTPPPPSPRLLMLYRIRRMMPRIDRGQRAEVQVLLRNASH
                     PNFDFFILVVLSSIIATLGLLTDSPAVIIGAMLVAPLMSPIIGLGLASLTADGRLLGS
                     AASALIRGALLAIVISFLISAINRLLPLIALQAQDLPGEVLARTHPSPIDLGIALAGG
                     MAAAFALAMPNISAALPGVAIATALMPPLCTVGIGLAWGRQDITGGALLLFVTNAVTI
                     AFAAILVFSALGFSPRRKEGAGLFPRSLQIAALLTLILMIPLTLFSVRFVQEATSDRT
                     IAQVIDENVRAFGDAELVEWDSNQEAGTLKINITMRTNKPLRLEDSIAFQKAVGGNLH
                     AQGVLGDNEKVQVVINQVLAERLDPLIPPTSTHTPTVTFTPTPGPSATPSLTPTPTPT
                     DTPSPT"
BASE COUNT          935 a         1194 c         1090 g          956 t
ORIGIN      
        1 ttttaggctg cgacgacttt aatgctcttg gggcgatggg cctcagcctt ggggacgcgc
       61 agatgcaaaa caccattttt aatgctagct tctgtcttgc tagaatcgag agcaaccggc
      121 aaggtgataa ctcgacggaa gcgaccagcg ggcagttcgt tcacaagaga tttaccatct
      181 tcatcggata cgctcttgaa ctcgccgcgc aaggttaccg tattgttaat aatctcgata
      241 ttgatgtcct cgggttcaag tcccggaacc agtgcaacga tatcataggc ttcatcttct
      301 gccttgacgt ccactgcaag catcatttcg cgctcgccat ttggggtctc atcaaggctc
      361 tcttcaaaca tgcgattcat catgttgcgc aaggttgcca ttcggcggta gggggaaata
      421 tagaatgtca tggattgtct cctcttttat attttgttag tttgtgtagg tcggctttcc
      481 acagtcgccg ggtcccaacg ggcatgcaaa cccggcctac atcatttttt agatgtcccg
      541 agtttaatgg atggatatca gaggagcaca agagaaatat agaattttta ttagaactgc
      601 aattggcgca tggcattctc cccagggttc tttaggcaaa tgcctatcgc aagaaactgg
      661 gatagacagc aaactacttc cgtcgggctg tgacaatcaa atatggggcc tggtgagaat
      721 atggcgagcg atcgaagtcc ccatataaag ccgcctgtga caacccggct gcctcgagct
      781 cggcgatgta gtcatctgtc gagagcagcg cgtgtcgcgc ctgcattgag aagcgttcca
      841 cgtttccgtc agggagcagg tgatcgtaat cccatcggac aacaaagtgc cctgtggtgc
      901 gcacccagct actgctcacc tgcaccggtt caccgtccag cgggtgagga aagctctcct
      961 caacctccgg ctggctccgg ccaagtaact tgcggagcaa agtcgggttg ggcaggctga
     1021 cggcaaaaag tccctgtggg gctaagtggc gctgcacaca atccagggtg gcgcgccgcg
     1081 cctcagtggt gagcgtgctg aaggtgttac aaggcagcaa aatcagggca aactgctgtc
     1141 ccaattcaaa agcagccata tctgcctgga tcgtgccaac ccgctcagcc agtgcaggtg
     1201 gtacattttc ccgcaatacg acgagcatat cggggtcact ttccagtccg agcacaaaat
     1261 gcccggcttg tgccagtggc aacaaaacac gaccactgcc gcatcccagc tccaggatgg
     1321 gatcaccttg caggcgcgcc agatctaacc agaaagggag atcttcctgg tgagcgttat
     1381 ggtgggcgtg atagagacga gcgagcatca atctccttga cgtatcaggc tgaaacgcat
     1441 tatactctct gagccggggc gtagcgcagt ccggtagcgc accgcgtttg ggacgcgggg
     1501 gccggcggtt cgagtccgcc cgccccgact ttggttggca atcatccttg tgtggaggaa
     1561 agccagtggc aaggatcaaa tttgatggag ttgtggaagc tgtccgctat aaacccagcg
     1621 gggagataga ttgggtgcgc gcctacgaac gtcgcggccc aaccttcagc gattacatct
     1681 tactcgaccg cgagacatta atcaagcgca ttaaagcagg caagcgctac gtctccggga
     1741 aacgcatccc ctatatggcc agcaccttcg aaacttcttc cccgattcgc ctggtgacca
     1801 atagtggaaa agagatcgtg gtcaccagtg atccgaaggc cactcaggac cgcttggagg
     1861 gcgtgccaac catataacag gtgatctatt aacgaaaaac ggtttatagg gcagcgttgc
     1921 caccgcccta taaaccgttt ttttaatctt tcccttgcag ggcgtgaaca gtaagctcta
     1981 cttgatctcg aagacgatgt tcacatccac catgagcacc aactgaccgg gagatacagg
     2041 cactggtgcg ccggctccgc ccccgccata gcccttggca tcgtaaatag gcactggcgc
     2101 accgccacca tagctgttga tcatctggat atctcccagg gttacaccag cggcggaggc
     2161 caactctttg gctgtagcca gcgcatcggc tactgccgac ttgcgcgctt cgcttagcgc
     2221 tttggaccga tctgccgcat cgaactggat gctgttaatc gagttcgctc ctgctcctac
     2281 agcagcgccg atcacatcgc ccaccttgga gatatctcgc agggtgacga aaacggtgtt
     2341 atcgacaaaa tagacaaccc ctgtgatctt ccctgtcggg tcatattgct gttgagggaa
     2401 aatgctgaaa ttggtggtct ggatgtcttt ggggtcgatg ttgaacttct tgaccgcatc
     2461 ggctaccttc ttcgagcgag cgttgttgct ggctacggct tccgcggcgt cggcattttc
     2521 agtgtgcaca ccgatgttaa tataggcgat atcaggggtc acaaaagcct tgccactccc
     2581 ggttacgttc agctggcgcg ggggagtttc cggccccgga ccttgttgag ttactgcaag
     2641 aacctccgta ccactgctga aggcgctcaa tagcgccgca acaagcaaca gagcaattcc
     2701 tagaaagact ttcttacgca tggggttata ctcctttgtt atgatgtact agcacttaga
     2761 cgcaaaacat tgcgatagtg ttccgtgatt ttcgatcact ttcatcataa cgtcaatccc
     2821 ccaaagaaag ttcccatacc ccaaagagta ccaaaatagg tagcatattg cgcccaatct
     2881 cttgttacaa ttgataataa tgggaatttg gccctaatgt gagctacatc accaacggcc
     2941 agtgatcaca gttaatcatt accagaaatt atcgcgtaat catgtctgaa aatctcacac
     3001 ccccaccccc atcaccccgt ctgttgatgc tgtaccgaat ccggcgcatg atgccacgca
     3061 ttgatcgtgg gcagcgcgcc gaagtccagg tgctgttgcg taacgcctca cacccaaatt
     3121 tcgatttctt catcctggta gtgctctcaa gcatcattgc cacgttgggc ttactcaccg
     3181 attcgccagc ggtgatcatc ggggcgatgc tggttgcgcc gcttatgtcg ccgatcatcg
     3241 gtctgggatt agcttcactg accgccgacg gtcgcctgct gggctccgcc gcatcggccc
     3301 tcatccgggg tgccctattg gctatagtga tttccttcct gatctccgca attaaccgac
     3361 tgctgccttt gattgcccta caagcgcagg atcttccggg agaggtgctg gctcgcacgc
     3421 atccctcacc gatcgactta ggtatcgccc tggctggcgg catggcagca gccttcgccc
     3481 tggcgatgcc caacatctcc gctgcgctcc ccggggtggc gattgctaca gcgctcatgc
     3541 cacccctttg cacggttggg attgggctgg cctggggacg gcaggacatc accgggggcg
     3601 ccttacttct cttcgtgact aatgcggtga cgatcgcctt tgcagccata ctggtcttct
     3661 ctgcgctggg cttcagcccg cgccgcaaag aaggtgccgg gctatttcct cgcagcttgc
     3721 agatcgcagc cctgctcacg cttatattga tgattccact gacattattc agcgtccgct
     3781 tcgtccaaga ggctactagt gaccgaacaa tcgcccaggt gatcgatgag aacgtgcgcg
     3841 ccttcggtga cgccgagcta gtcgagtggg attcgaacca ggaggctggc accctaaaaa
     3901 tcaacatcac catgaggaca aacaagccct tgcgcctcga agacagcatc gcgttccaga
     3961 aagctgtggg aggtaattta catgcccagg gtgtcttggg cgacaacgag aaagtccagg
     4021 tggttatcaa ccaggtgctg gctgaacgac tcgacccgct gataccgcca acgtctacac
     4081 acacgccaac ggtcaccttc acccccacac ctggaccgag cgcgacgcca tcattgacgc
     4141 ccacccccac ccccaccgat accccctcgc ccacc
//
LOCUS       DBNHOH010000559         2264 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_738520, whole genome shotgun sequence.
ACCESSION   DBNHOH010000559 DBNHOH010000000
VERSION     DBNHOH010000559.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_738520"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..1003
                     /locus_tag="AC16V7_09725"
     CDS             26..1003
                     /locus_tag="AC16V7_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HIR6257438.1"
                     /translation="MPEPFSPKPKKMLDQLREVIRIKHYSYSTEKNYVHWAKRYILFH
                     NKRHPAEMGAPEIEAFLSHLAQEVNVSASTQNQAFNAILFLYRNVLKIELTTPVYALR
                     AKRSQHLPTVLSKNEVNQVLGGMQGLHQLMARLLYGCGLRLMECLRLRVKDIDFEQCQ
                     IIVREGKGEKDRLTMLPVTVVEPLKAQIAFVQKQHMRDLEQGFGSVELPFALARKYPH
                     ADKELAWQYIFPSDRLSTDPRSGITRRHHLDPSGLQRAVKAAATLAKINKPISPHTFR
                     HCFATHLLEAGYDIRTVQELLGHKDVKTTMIYTHVLKSGPKAVRSPLDE"
     gene            complement(1082..1585)
                     /locus_tag="AC16V7_09730"
     CDS             complement(1082..1585)
                     /locus_tag="AC16V7_09730"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6257439.1"
                     /translation="MSAISYRPATESDFAALGAMYEKLNTFFYEVGYLLPRTENVGEL
                     WLDSFRRTLGRFSNVIIAEREGRPLGFILCRLKRLPAHMGGLLVGEVSDVWVEGEARR
                     ASVGEALVQAGVEWLRQQGVHSVEVQVLSENQASLKFFESLGFKLELRALRLILEKDA
                     QQVARFE"
     gene            complement(1593..>2264)
                     /locus_tag="AC16V7_09735"
     CDS             complement(1593..>2264)
                     /locus_tag="AC16V7_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class-II aminoacyl-tRNA synthetase family
                     protein"
                     /protein_id="HIR6257440.1"
                     /translation="HLRTDLDVIDHFAREASCGEGGELAAPPGSFAPVRALLSPAVCY
                     HLYFSLADQPLPGEGVIATSVGNCFRHEAINLTSLERLWNFTMREVIFVGSKEFVLDH
                     RETARQRTAKFLGDIGLAYRVESANDPFFIGEFRKQVAFQSAFQLKYEIRAHLPFKDS
                     TLAVGSYNYHQDFFGRRLNITLPDGSPTHTGCVAFGLERVAYAFLAQFGLDPRDWPDV
                     VRKDF"
BASE COUNT          510 a          661 c          639 g          454 t
ORIGIN      
        1 tccccccctc ataagcgagt cgcgtatgcc tgagccattt tcgccaaagc ccaaaaaaat
       61 gctggaccaa ctacgcgaag tcatccgcat caagcattat tcgtattcca ccgaaaaaaa
      121 ctatgtccat tgggcaaagc gctatattct ctttcataac aagcgccacc ctgccgaaat
      181 gggcgcgccg gagatcgaag cattcctttc ccacctggcc caggaagtca atgtctcggc
      241 atccacccag aaccaggctt ttaatgccat tcttttcctc tatcgcaacg tattgaaaat
      301 tgaactgacg accccggtct atgccttgcg cgccaaacgc agccaacact tgccgaccgt
      361 gctttccaag aatgaggtga accaggtgtt gggcgggatg caagggctgc accagctcat
      421 ggctcgcctg ttgtatggct gcggtttgcg cttgatggaa tgcctgcgcc tgcgggtcaa
      481 ggacattgac ttcgagcaat gccaaatcat tgttcgcgaa ggcaagggcg agaaagatcg
      541 cttgacgatg ctgcccgtca ccgtggtgga gccgctgaaa gctcagattg cttttgtgca
      601 gaagcagcac atgcgtgatt tagagcaagg atttggttcg gtggaactgc cttttgcctt
      661 ggcgcgcaaa tacccgcatg cagataagga attggcctgg cagtatatct ttccctctga
      721 tcgcctgtcc acggatccgc gcagtgggat tacccgccgc caccatttgg atcccagcgg
      781 gttgcagcgg gcggtgaagg cggctgcaac cctggcaaaa attaacaagc caatcagccc
      841 gcataccttc cgtcactgct ttgccaccca cctgctcgaa gcaggctatg atatccgcac
      901 tgtgcaggaa ctactcggcc acaaggatgt caaaacgacg atgatttaca cccatgtact
      961 caagagcggg ccgaaggcgg tgcgtagtcc actggatgag tgaggctgct ttttcaaggt
     1021 tgtccctgct cttgtctggg gttttaatcc ttgtacgggc tttgtctagc tgtgccagat
     1081 cctactcaaa tcgtgctacc tgctgagcat ctttttccag gatcagccgg agcgcgcgca
     1141 gctcgagctt gaaccccagg ctctcgaaga acttcaggct agcctggttt tcgctgagca
     1201 cctggacttc cacggagtgc acgccctgct ggcgcagcca ctccactcca gcctgcacaa
     1261 gcgcttcgcc cacgctggcc cggcgcgcct cgccctccac ccagacatca ctcacctcgc
     1321 ctaccagcag gccacccata tgcgctggca agcgctttag acggcacaag atgaatccca
     1381 gtggacgacc ttcccgttca gcgatgatga cattgctgaa gcgccctaga gtgcggcgga
     1441 aagaatccag ccaaagctcg cccacattct ctgtgcgggg cagcaggtag cccacctcat
     1501 agaaaaaagt attcagcttc tcgtacatag cgccgagggc ggcgaaatcg gattcggtgg
     1561 ctggtcggta agatatagcg ctcatcgcaa aactagaagt cctttcgaac aacgtccggc
     1621 caatcccgcg ggtccaggcc gaactgagcc aggaaggcgt aagccacgcg ctccaggccg
     1681 aaagccacgc agccggtatg ggtaggggat ccatcgggca gggtgatatt caggcggcga
     1741 ccgaagaaat cctggtggta attgtatgag cccacggcaa gcgtagagtc tttgaaaggc
     1801 aggtgggcgc ggatctcgta cttgagctgg aaggcgctct ggaaggcaac ctgcttgcgg
     1861 aactcgccga tgaagaaagg atcgttggcg ctctccacgc gataggccag gccgatatca
     1921 cccaagaatt ttgccgtgcg ctggcgggca gtctcgcgat ggtctagcac aaactccttg
     1981 gagccgacga aaatcacctc gcgcatggtg aaattccaca ggcgctcaag ggaggtcagg
     2041 ttgatggctt catggcggaa gcagttgccc accgacgtgg cgatgactcc ctcgccgggt
     2101 aagggctgat cggccaggga aaaatacagg tggtaacaaa ccgccgggga gagcagggcg
     2161 cgaaccgggg caaaggatcc cggcggggct gccagctcgc cgccttcgcc gcaggaggcc
     2221 tcccgggcga agtgatcgat cacatccagg tcggtgcgca ggtg
//
LOCUS       DBNHOH010000560         3206 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_353163, whole genome shotgun sequence.
ACCESSION   DBNHOH010000560 DBNHOH010000000
VERSION     DBNHOH010000560.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_353163"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1297)
                     /locus_tag="AC16V7_09740"
     CDS             complement(95..1297)
                     /locus_tag="AC16V7_09740"
                     /inference="COORDINATES: protein motif:HMM:NF020246.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HIR6257441.1"
                     /translation="MQNLSRRFVTLILILALVQVSCNLLQRLPPPAATPTLEVSPTSP
                     ATPTLVPSATATPTPTITPTPLPTSTPTQPPLADLGTPLPAFLEPISINNVTRVNPLA
                     ELRKEAITKLAWSLDSTTLAAGGNSEVDFFDIRTLTKQRTLKVDEGLVSFGLSPDGRY
                     LASGNNTGSEQSGYTGSISFWRLSDEERLFIFYLDRRGVSDLSFSPNGRTFAAAVTSK
                     EYIDNGFVFWNTQTFEITRTLRTGGALDMAFSPDGRYIASTPDRFAIRLWEMRYGQYL
                     YELPTSFTGAVNTLAFSPDGSILATGHYDGVIRLWDVAKGELLQEFSAIGVVESLVFS
                     PDGTLLASGEGYTSTAIRLWDLASGQNLRTLEGHTHAVDNLAFSSDGRLLASGSYDGT
                     LRLWGVTP"
     gene            complement(1345..>3206)
                     /locus_tag="AC16V7_09745"
     CDS             complement(1345..>3206)
                     /locus_tag="AC16V7_09745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0045910 - negative regulation of DNA
                     recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HIR6257442.1"
                     /translation="GDPHNAQFLQESLITQRDGRYVLPLRSEFKGRIKSIVHDQSASG
                     ATLFVEPLAVVELNNEYRELQLTERDEERRILAELSQHVGRYAAEILQTVEAIAKLDV
                     FFASAKYAEDLRASEPTLHDIHPRRDSQHPGTIIRLYQARHPLLDPQTVVPIDVELDP
                     QTYAMVITGPNTGGKTVTLKTVGLLALMAQAGLHIPAQTGSEISIFHDIFADIGDEQS
                     IEQSLSTFSGHVTNIIHILDEADRRALIILDELGAGTDPQEGAALARALLTHLLERGI
                     TTLVTTHHPELKAYAHATPGVVNASVEFDLETLRPTYHLTVGLPGRSNALAIAQRLGM
                     PDVIIDTARSELSPSDLRAEDLLDEIHRQRDLSRQARLTAENARNQAEKLRLELAERL
                     EKIEDERLQVLEKARLEAEAQIQVLQDELRELRRQLARARQPLEALRSVEEQIEDLEQ
                     GVDKQIERRIPKVDETPIYRPVRLGDKVRLRTLNTQGVVTSLSEEEAEIQVGRLRVRT
                     RIADLQPLDDEAQPLSEAVSMGDHPSAELTASPGFELDLRGQRADEALEILERYLDSA
                     YLAGLPFVRIIHGKGTGKLRQAVRQALQSHPNVRSFEAGGDKEGGEGVTVAKL"
BASE COUNT          673 a          820 c          957 g          756 t
ORIGIN      
        1 tgcttggttt ggattggtgg atgagtgggg gtgggtgttg tcggcaggct ggcgcaagct
       61 gcaaggccta atgcggccag caccggcaca accctcaagg cgtcactccc caaaggcgta
      121 aggtgccgtc gtaagagccc gaggcgagca agcgaccatc ggatgagaag gccagattgt
      181 ctactgcatg ggtgtggcct tccaatgtgc gcaaattctg accactcgcc aggtcccaca
      241 agcggatggc agtgcttgtg taaccttccc cagaagccag cagggtaccg tcgggactga
      301 agacaaggct ttccactacc ccaatggcgc taaattcctg aagtagctca cctttggcta
      361 catcccacag gcggatgacg ccatcatagt gcccggttgc caggatactt ccgtcaggcg
      421 aaaaagccaa ggtgttgacc gctccggtaa aggaggtggg cagttcatac aggtactgcc
      481 cgtatcgcat ttcccataag cggatggcga atcgatcggg tgtagaggcg atatagcgcc
      541 catctggcga gaaggccatg tctagcgcac cgccggtgcg taatgtgcga gtgatctcaa
      601 aggtttgcgt gttccagaac acgaagccgt tgtctatata ttctttactg gtcacggctg
      661 cggcaaaggt tctgccattg ggggagaagg ataaatcgct aacccctcgc cgatccaagt
      721 aaaagataaa caaacgctcc tcgtcggata ggcgccaaaa agagatgctc ccagtatagc
      781 ctgactgctc tgaccccgtg ttattcccag aggcaagata ccgcccatca gggcttaggc
      841 cgaaactcac caaaccctca tctaccttta aagtcctctg ttttgtcagc gtgcgaatgt
      901 cgaaaaagtc cacctcggaa ttgccgcctg cggctagagt tgtgctgtct agtgaccagg
      961 ctagtttggt gatagcttcc ttgcgcaatt ctgccagcgg atttacccgg gtgacgttgt
     1021 ttatgctgat gggctctaga aatgcgggta atggagttcc taggtcggcg agaggcggct
     1081 gtgtgggcgt tgaggtaggc aaaggggtag gggtgatggt tggagtcggc gtggcggtgg
     1141 ctgagggcac caaagtgggc gttgccggtg atgtgggcga tacctctaag gttggggtag
     1201 ccgcgggagg cggtaagcgt tgcagtagat tgcagctcac ttggacgagc gccaggatga
     1261 gtattaaggt gacaaatcgc cgggatagat tttgcatatg cgcgttccaa ctggcaaaga
     1321 tttttctcac tgctctcagt cagtctacaa ctttgccaca gtgacccctt ctccgccctc
     1381 tttgtctcct ccggcttcga aagagcgcac atttgggtga ctctgaagcg cttggcgtac
     1441 cgcttggcgc aacttgccag tgcccttacc atggatgatg cgaacgaagg gtaggccggc
     1501 caggtaggca gagtccaggt agcgttctag gatctccaag gcttcatcgg cgcgctgtcc
     1561 gcgcaggtct agctcaaagc caggagatgc ggtcagttcg gcagaaggat ggtcacccat
     1621 ggacaccgct tcggatagcg gttgggcctc gtcgtccaac ggttgcaggt cagctatgcg
     1681 tgttcggacg cgcaacctgc ccacctgaat ctcggcctcc tcttcactta atgaagtaac
     1741 cacgccttgg gtattcagag ttcgcagtcg gactttatca cccagtcgaa cggggcggta
     1801 aattggtgtt tcatctactt taggaattcg gcgttcgatt tgtttatcta caccctgttc
     1861 cagatcttca atttgttctt ctacgcttcg cagggcttcc agtggttgtc gcgcccgggc
     1921 aagctggcgc cgaagctcgc gcagctcatc ctgtaggact tggatttgag cctcggcttc
     1981 caggcgcgct ttctctaaaa cctgcaagcg ctcatcttcg atcttctcca gacgctctgc
     2041 cagttcgagg cgaagtttct cggcttggtt gcgggcattt tcagcggtga gacgagcctg
     2101 acgagaaaga tcgcgttggc gatgaatttc gtccaggaga tcttcggctc gcaggtcgct
     2161 tggactgagc tccgagcgcg cagtgtcaat gataacatca ggcatgccca ggcgctgggc
     2221 aatagccagg gcattcgatc ggcctggaag gccgacagtc aggtggtagg tcggccgcag
     2281 tgtctccaga tcgaactcta cactggcatt cacgacacct ggcgtagcat gtgcataggc
     2341 tttcaactca gggtggtgcg tagtgactaa agttgtgatt cctcgctcca gcaggtgggt
     2401 gagcagtgcc cgcgctaggg cagcgccttc ttgcggatcg gtgccggcgc ccagttcatc
     2461 aagaatgatt aaggctcggc ggtcagcttc atccaagatg tggataatat tggtgacatg
     2521 ccccgaaaaa gtggataggg attgttctat cgattgttca tcgccgatat cggcgaaaat
     2581 atcgtggaag atgctgatct cagagccggt ttgagctggt atgtgcaatc cggcctgtgc
     2641 catcagcgcc aacagaccga cggtcttgag tgtgactgtc ttgccgccgg tattcggacc
     2701 ggtaatgacc attgcatagg tctgcgggtc aagctcaaca tcgatgggca ccacggtttg
     2761 gggatctaat agaggatgtc gggcctggta tagtcggatg atcgtgccag ggtgttggga
     2821 atcccgccgt gggtggatgt catgtagtgt aggctcggat gcgcgcaggt cttctgcgta
     2881 tttcgcgctg gcgaagaaaa catccagctt ggcgatggct tccacagttt gcaaaatttc
     2941 agccgcgtaa cgacccacgt gctgagatag ctcagcaagg atgcgccgct cttcatcgcg
     3001 ttcggtcaat tgcagctcac ggtattcgtt gtttagctca accacggcca gtggctcgac
     3061 aaacagtgtt gctccagatg cagattgatc gtggacgatg gacttgatcc gacctttaaa
     3121 ttccgacctg agtggcagga cgtagcgacc atctctttgt gtaatgaggg attcttgcaa
     3181 gaactgggca ttatgcggat cgccca
//
LOCUS       DBNHOH010000561         3025 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_481993, whole genome shotgun sequence.
ACCESSION   DBNHOH010000561 DBNHOH010000000
VERSION     DBNHOH010000561.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_481993"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1651)
                     /locus_tag="AC16V7_09750"
     CDS             complement(<1..1651)
                     /locus_tag="AC16V7_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257443.1"
                     /translation="MLTLRRWLRLGLPLGVAGTFLALWLVCIQSMTVAIARANHRGHL
                     TWNHLNSISIHDIQGASHKSPLENQMVAGVKGVVTALRKRGFYMQEPAPDSDEATSEG
                     IYVYLNDTPSVQVGEQVSVTGMVLEETPFSLSPGSLSVTWITTTISNIQVLSTGLELP
                     EPVLVGQGGRAPPDQIIEDDSDDDVNDSKIFDPDSDGIDFYESLEGMRVKVLDPVAVS
                     GTSQDGVIVVLGDGGVLAGVRTPRGGLVVQPDDFNPERILVEDAIVSSEPRVNVGATF
                     SGVITGVMDYSLGNFKLFNTEPFPSITTTGVTSETVSEASPDQLSVVTLNTKNLDPKE
                     SPSKYARLARQIVGYLLSPDILVLEEIQDSNGSLNDAVVTATLTYSMLVTAIQEAGGP
                     LYQVRQIDPLDDQDGGQLGGNIRVGFVFRTDRGLSFVDRPGGDAITPVTATLGTNGSQ
                     LSISPGRVDPLNQAFNESRKPLAGEFIFKGHKLILIACHLNSRSDDSLLFGRYQPPRQ
                     YSQAQRIQQAGAVNAFVHHILTLDPLANVIVLGDFNDFPFST"
     gene            1747..1820
                     /locus_tag="AC16V7_09755"
     tRNA            1747..1820
                     /locus_tag="AC16V7_09755"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1781..1783,aa:Arg,seq:gcg)
     gene            2023..2382
                     /locus_tag="AC16V7_09760"
     CDS             2023..2382
                     /locus_tag="AC16V7_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257444.1"
                     /translation="MIGELLNNRYLIQSEIGRGGFGVVYRANDTLLEWDVAISGVVTA
                     VPLLMFATAAHPAVDGGLATVHRHDLPVPAGHPGLTRTVQPGAAEGVCDHLDTAAGVL
                     HGGLTGSHRMALQAHSH"
     gene            2521..2991
                     /locus_tag="AC16V7_09765"
     CDS             2521..2991
                     /locus_tag="AC16V7_09765"
                     /inference="COORDINATES: protein motif:HMM:NF019095.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HIR6257445.1"
                     /translation="MSIVKNKLTYRFLLMLALAALLAAAALPVLANGGRPLGAMLNGP
                     NEVPVSGDPDGSGWAHVTLNQGQNEVCWHIQVEDITLPARAAHIHSGAAGVPGGVVVG
                     LSAPKEDGTAMGCTTAEADLIKDIRQNPGSYYVNVHNTDYPGGALRGQLFKLDD"
BASE COUNT          707 a          821 c          836 g          661 t
ORIGIN      
        1 tggttgagaa aggaaagtcg ttaaaatccc ccaatacgat cacatttgcc agcgggtcca
       61 gcgtaaggat gtgatgaaca aaggcattga ccgcgccagc ctgctgtatg cgctgcgcct
      121 gggagtattg ccggggtggt tggtagcgcc caaataaaag gctatcgtcg cttctcgagt
      181 tgaggtggca agcgatgagg attagcttgt gacccttgaa gataaactca cctgccaggg
      241 gcttgcggct ttcattgaag gcctggttca gtgggtcaac acgtcccggg ctgatagaga
      301 gttgggaccc gttggtaccc agcgtggctg tgaccggagt gatggcatcc cctccagggc
      361 ggtcaacaaa ggataaaccc cggtcggtgc ggaagacaaa tccaacgcga atattgccgc
      421 caagctggcc tccatcctgg tcatccaggg gatcaatctg ccgaacctgg taaagcggtc
      481 cgcccgcctc ttggatggca gtcactagca tcgagtaggt gagcgtggcg gtcaccacgg
      541 catcattcaa gctgccattg ctgtcctgta tctcctccag gaccaggatg tctggagaaa
      601 ggagatatcc cacaatctgc ctggctagtc tggcgtattt gctagggctc tccttggggt
      661 cgaggttctt ggtattaagg gtaacgacgc tgagctggtc gggtgaggct tcggataccg
      721 tttccgaggt tacccccgtg gtggtgattg aaggaaaggg ttcggtattg aacagcttga
      781 aattaccaag gctgtaatcc ataacgccag tgatcacacc gctgaaggtg gctcctacgt
      841 tcacccgcgg ctcggaagat acaatcgcat cttctaccag aatgcgctcc gggttgaaat
      901 catccggctg aacaaccaag ccaccgcgtg gtgtgcgcac ccctgccaat accccaccgt
      961 cgcccaacac gacgatcacc ccatcctggc tcgtaccgct gacggcaacc gggtcgagca
     1021 ctttcacacg catgccctcc aagctctcgt aaaaatctat cccgtcagaa tcaggatcaa
     1081 agattttgct atcgtttaca tcgtcatcgc tatcgtcttc gataatctgg tcaggtggcg
     1141 cccgtccccc ttgaccgacc agcacaggtt cgggtagctc cagtccagtt gacaggactt
     1201 gaatattgga aattgtcgtg gtaatccagg tgaccgacag gctgccggga gaaagactga
     1261 acggggtttc ttcaagcacc atgccagtga ccgatacctg ctccccaacc tggacagagg
     1321 gcgtgtcgtt caggtagaca taaatccctt ctgaggtagc ttcgtcgcta tccggcgcag
     1381 gttcctgcat gtaaaaaccg cgcttcctca gagcggtaac gacacctttg acacccgcga
     1441 ccatctggtt ttccaacggt gatttatgac ttgcgccttg aatgtcgtgg atcgagatgg
     1501 aattcaagtg gttccaggtt aagtgacccc tgtgattagc ccgcgcgatt gcaacagtca
     1561 tcgactgaat gcaaaccagc caaagcgcca ggaaagtacc agccacgcca agtggcaggc
     1621 ccagccgcag ccatcggcgc agggtgagca tactttaagt atatattgaa tcccccagaa
     1681 tgtatcgacc ttgaccgtgc cagatatatg cagtacaatc acgggcgcaa gaaatcggtt
     1741 tcgattgccc tcgtagctca gtggattaga gcacttgctt gcggagcaag gggtcgcgtg
     1801 ttcgagtcgc gccgggggcg ctgatttttt aaggcggaga ttggtggaat acctttctcc
     1861 gctatttttt ctgacaagtc accatttcgc cctccagggg gaattcgctc aaatgcgata
     1921 gctccacttg caagcctgaa cataagcaca aaggattata attaggctaa aatttatcct
     1981 attctcgata ttgagccagc aaaagagggt atcagggatc taatgatcgg tgagctttta
     2041 aataatcgct atttgataca atccgagatt ggtcgcgggg gattcggcgt ggtctaccgc
     2101 gccaatgaca ccctgctcga gtgggatgtg gcgatctctg gcgtcgtcac tgctgtaccg
     2161 ctgctgatgt tcgccacggc ggcgcatccc gctgtggatg gtgggcttgc tacagtacat
     2221 cgccacgacc tgccagttcc tgctgggcat ccaggtctaa cgcgaaccgt tcagccaggc
     2281 gcggctgaag gggtttgtga tcatctggac accgctgctg gtgttctcca tggagggctt
     2341 actggctcac ataggatggc gctccaagcg cacagccact gacacaagca cagccaccta
     2401 acagcaaata cagactcaat ttgctagaaa accaatccaa cctggacctt tttgcgtaat
     2461 atatggcaga acgagcgtaa aagcgcggtt caatacaacc accaaaggag aggaattcca
     2521 atgtctatcg taaaaaacaa actcacgtat cgatttttat tgatgctggc gctggctgca
     2581 ttgctggcag cggcagcctt gccagtcctg gcgaacggtg gtcgcccatt gggcgccatg
     2641 ctgaacggcc ccaacgaggt gccggtgagc ggtgatccag atggaagcgg ctgggcgcat
     2701 gtgaccctta accaaggtca gaatgaagtg tgctggcaca tccaggtcga ggacataacg
     2761 ctgccggcca gggctgcaca catccattcc ggagcggccg gagtgcccgg tggggtcgtg
     2821 gttggcctat cagctcccaa agaagatggc accgccatgg ggtgcacgac tgcagaggca
     2881 gacctgatca aagacatccg ccagaacccg ggcagctact atgtgaacgt gcacaacact
     2941 gattatcccg gcggcgccct gcgcggacag ctcttcaaac tggacgactg agccaagcaa
     3001 ccaataaaca gctggtactc ctctc
//
LOCUS       DBNHOH010000562         1943 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1379373, whole genome shotgun sequence.
ACCESSION   DBNHOH010000562 DBNHOH010000000
VERSION     DBNHOH010000562.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1379373"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="AC16V7_09770"
     CDS             <1..214
                     /locus_tag="AC16V7_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257446.1"
                     /translation="LLYTRVDFHTAGNSPAYSTGAGLLLLGLFHNLQNVRQRWNWEPA
                     QPVRALSFPSTRRDRWRRPTTRRPYR"
     gene            224..505
                     /locus_tag="AC16V7_09775"
     CDS             224..505
                     /locus_tag="AC16V7_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257447.1"
                     /translation="MPTQFDFDPRTEGLLAHIQRMLPDLPRILGSEWPAFENRLQALL
                     RQLEQPDVTPVVVEAQVLDLFAAYPRVIRERLRFSQICQIGSRGRKLSG"
     gene            502..1803
                     /locus_tag="AC16V7_09780"
     CDS             502..1803
                     /locus_tag="AC16V7_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015159588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HIR6257448.1"
                     /translation="MTNKKLRNPDHVRRRNAPQANNEALEQRLQELLTPAVFSQLGYY
                     RQLGMRDRILTLPVMVALTLTILWRQVPSVRELTRMLAREDLLWCKAVKVSQPSLSER
                     LLVFPAELFERVYKALLPKLKARWEARQRRPVPQSIQIASSHFDQIFVADGSTLEALF
                     RKLDVLADSPVGQLAGKMCVVIDLVRQLPVELWFDEDARAFDTRFIPDLLTLVREKTL
                     LILDRGFYDFRFFASLIDQGSHFITRLKSNTKTVVVKRLSYTDSVKDLIVHLGTGQNG
                     APILTLRMIEVRFGQQWYRYITSVLDPEVLPPFVVADLYRRRWRIEEAFNTAKRLLNL
                     AYLWTGSENGIKLQIWATWLFYVVLVDLGDAIADEMSLPFDQISLEMLHRAFYHFIQA
                     FDRGKASDPITYFTAPENQDLGIVKAIRKSIPRLNLQPYPT"
BASE COUNT          437 a          538 c          512 g          456 t
ORIGIN      
        1 tctgctttac acccgcgttg atttccatac ggcgggaaat agccctgcct actcaaccgg
       61 cgctggcctt ctgttgctgg gtctatttca taatctacaa aatgtacgac agcggtggaa
      121 ctgggaacca gcgcaaccgg tccgtgcact cagctttcca tccacgcgca gggatcgatg
      181 gcgtcgtccg acgacacgtc ggccctacag ataggaggtc tatatgccaa cccaattcga
      241 ttttgacccg cgtacagaag gccttctggc ccacatccag cgcatgctgc cggacctgcc
      301 ccgcattcta ggcagcgagt ggcctgcctt cgagaatcgc cttcaagccc tgctccgtca
      361 actggagcag cctgacgtaa cccctgtggt ggtcgaggcc caggtgttgg acctcttcgc
      421 ggcctacccc cgggtcattc gtgaaaggtt aaggttcagc cagatttgtc aaattggcag
      481 tagaggccgt aaattgtcgg gatgacaaac aaaaaactgc gaaatcccga tcatgtaagg
      541 cgacgaaatg cgccgcaggc caacaatgaa gcgctggaac agcggctcca agagttgctc
      601 acgccagcag tgttcagtca gttggggtac taccgacagt tgggcatgcg cgaccgcatc
      661 cttacgttgc cggtgatggt ggccttgacc ctaacgatct tgtggcggca agttccttca
      721 gtgcgtgaac tgacccgcat gcttgcccga gaggatctgc tctggtgcaa ggcagtcaag
      781 gtgagccaac catctttgag tgaacggcta ctggtctttc cagcagagtt attcgagcgg
      841 gtgtacaagg cgttgctgcc caaactgaaa gccaggtggg aggcccgtca acgcagacct
      901 gttccccaat cgatccagat cgccagcagc cactttgacc aaatattcgt ggccgatggc
      961 tcaaccttgg aggcgctgtt ccgcaagctg gatgtcctgg ctgattcgcc ggtcggccaa
     1021 ttggcgggca aaatgtgcgt cgtcatcgac ctggtgcgtc aactccccgt tgaactctgg
     1081 tttgatgaag atgcccgagc ctttgacacc aggtttattc ccgatctgct aacgctcgtt
     1141 agggaaaaga cgctcttgat tctagatcga ggcttctatg acttccgttt ctttgccagc
     1201 ttgatcgacc agggtagcca tttcatcacc cggttgaaga gcaataccaa aacggtggtc
     1261 gtcaagcggt tgagctatac agattcagtc aaggacttga tcgtgcatct gggcacgggc
     1321 cagaatggag cgccgatttt gaccctgcgc atgatcgaag tgcgctttgg tcagcaatgg
     1381 tatcgataca taacatcggt tctggaccct gaagtattgc ctccttttgt cgtggctgac
     1441 ctctaccgcc gccgctggcg gatcgaagaa gcattcaaca cggcgaaacg actgctcaac
     1501 ctggcatatc tatggaccgg ttctgagaac ggcatcaagt tacagatttg ggcgacctgg
     1561 ctcttctatg tggtcttggt cgacctgggc gatgcgattg cggatgaaat gagcttaccc
     1621 ttcgaccaga tttctctgga aatgctccat cgggccttct atcacttcat ccaagccttc
     1681 gatcgaggta aggcatctga tccaattacc tatttcactg caccagaaaa ccaagatctg
     1741 ggcatcgtta aggccattcg caagtcaatt ccaagactca atctccaacc ttatccgact
     1801 tgacaagtac ctatttcgct taacctttca cgaatggtgg agataatgaa tagcatccgt
     1861 gcatattgtg ggtctacggc atctgcgcgc ccacggcctc cacatagacg gcataaatcg
     1921 attggctggc ggcgataaag agc
//
LOCUS       DBNHOH010000563         2354 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_482114, whole genome shotgun sequence.
ACCESSION   DBNHOH010000563 DBNHOH010000000
VERSION     DBNHOH010000563.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_482114"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1310)
                     /locus_tag="AC16V7_09785"
     CDS             complement(<1..1310)
                     /locus_tag="AC16V7_09785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6257449.1"
                     /translation="MIETQKHKSPIHRSFWLEQVAGDAPEEALLMGDQRADVAIIGGG
                     YVGLWTALRIKEIDAGCDVVVLEQDICGGGASGRNGGFVLSYWPKLASLAKICGEQDA
                     IQIARDSEEAIDSIRDFCEAYQIEAHFRRGGWIWTATSQSQMDSWDSVVRLCDKLGVD
                     AFQELSPQEVAQRSGSPAHRAGVFEASAATVQPAALVRGMRRVALQRGVRIFEGTRVQ
                     AFGRTHPIAIKTNAGVLTAEKLVIASNAWAAGIRELSRSIVAVTSDMVITAPVPDELQ
                     NLGWVGGECITDSLMMVDYYHATQEGRVAFGKGGWGIAYGGNIGKDFDRNEQRAQNVI
                     AHLHRYYPSLRQVPLTHDWSGPIDRTPNSLPVLGRLGGRNHILYGIGWSGNGVGPSVI
                     GGRILASLALERDDDWSRYPLVEWSARRFPPEPIRYIGAHFVRNA"
     gene            complement(1340..1747)
                     /locus_tag="AC16V7_09790"
     CDS             complement(1340..1747)
                     /locus_tag="AC16V7_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008869047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HIR6257450.1"
                     /translation="MTLSYYIKLYISALAAFLLIDAIWLGLVARGFYRKYLGFLLSPT
                     PNWAAAIIFYLLFIVGLVVFAIAPGLQAASLGKALLLGALFGLLTYATYDLTNLATIK
                     DWPLIVTLVDLVWGVILSSSVTWVAFQVGRWLG"
     gene            1989..>2354
                     /locus_tag="AC16V7_09795"
     CDS             1989..>2354
                     /locus_tag="AC16V7_09795"
                     /inference="COORDINATES: protein motif:HMM:NF015587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HIR6257451.1"
                     /translation="MRNNLVSRAYDQINQFSGGALEILKRTMNSLSRERAGQAAAGLA
                     YYAFFSLFPLLLLLVVGSSFFFESDQASQQVVEFISQALPVSTTLLERNIAQVLELRG
                     PVSAIGLISLLWSATSVFSA"
BASE COUNT          561 a          669 c          626 g          498 t
ORIGIN      
        1 gcattgcgca caaagtgtgc gccgatatag cgtattggct caggtggaaa tcgccttgca
       61 gaccactcca caagcggata gcgactccaa tcatcatccc tctcaagcgc aaggctggca
      121 aggattcttc caccgattac actcggcccg actccattcc cgctccagcc tatcccgtac
      181 aggatgtgat tgcgcccacc cagcctccca agcaccggca gactgttggg cgtgcggtca
      241 atggggccag accaatcgtg cgtaaggggc acctgtctca ggctggggta gtaacggtgc
      301 agatgggcga tgacgttctg agcacgctgc tcgttccggt cgaaatcttt gccaatattg
      361 ccgccatagg caatccccca gccgccctta ccaaaagcga cccgaccttc ttgtgtggcg
      421 tgatagtaat cgaccatcat tagcgaatcg gtgatgcact cgccgcccac ccagcccaga
      481 ttttgcagtt catctggaac aggggcggta atcaccatat cgctggtgac ggcgacaatg
      541 ctccggctta actcgcggat gcccgccgcc caggcattgg aggcgatgac gagcttttcg
      601 gcggtcaaca ctccagcatt ggttttgatg gcgatgggat gtgttcggcc gaaggcctgg
      661 acgcgcgtgc cttcaaaaat gcgcacgcca cgctggagcg caacccggcg cattccacgc
      721 accagagcag cgggctggac ggtggcggca ctggcttcaa aaacacctgc acgatgcgca
      781 ggtgaccctg agcgctgagc gacctcttgc ggggagagct cttgaaaggc atccactccc
      841 aacttgtcgc acaagcgcac cacgctgtcc caggagtcca tctgcgactg ggaggtggca
      901 gtccatatcc agccgccgcg ccgaaaatgc gcctcgattt gataggcctc gcaaaagtca
      961 cggatggaat caatagcttc ttcagaatct cgcgcgattt gtatggcgtc ttgctcaccg
     1021 cagattttgg caagcgaggc gagctttggc caataagaca gcacaaaccc accattgcga
     1081 cccgaagcgc cccctccaca aatatcctgc tctaacacca ccacatcaca acctgcatct
     1141 atttctttaa tgcggagtgc agtccacaaa cctacgtacc cgccaccgat gatagcgaca
     1201 tcggcacgct gatcgcccat tagaagcgct tcttctgggg catcacccgc cacctgctcg
     1261 agccaaaatg agcggtggat tggtgattta tgtttttgag tctcgatcat agccttgggt
     1321 ggcctccctc aacaaggatt tatcccaacc acctgccgac ctggaaagcc acccaggtca
     1381 cggaactgga gaggatcacc ccccatacca aatctaccag cgtgacgatt agaggccaat
     1441 ccttgatggt cgccaggttg gtcaggtcgt aggtggcgta ggtgagcaag ccgaacagcg
     1501 cgcccagcaa cagggctttg cccagcgatg cggcttgtaa tcccggtgca atggcaaaca
     1561 ccaccagccc tacgataaat aacagataaa agatgatggc tgctgcccaa ttaggggtcg
     1621 ggcttagcag gaatcctaaa tatttgcggt aaaatccgcg tgcaaccagg cctaaccaaa
     1681 tcgcatcaat gagcaagaag gcagccaggg ctgagatgta gagcttgatg tagtaagata
     1741 gggtcatgag gcctccatta ggtcttacat agagcttgga tagctagggc tttggaagta
     1801 tggaagtgcg ctggcaatca atttagggcc gaatcgtatt gataagtcat tggttcacac
     1861 caggcctaaa gcagagcacg ccatacagca aacgttagat ttttcacgca ccgtcaagac
     1921 gatgacttga aacgcctgat agatattata cgtcgaacgt aatatttacg aatcaggaaa
     1981 tggcgaggat gcgaaataat cttgtgtcac gggcttatga ccagatcaat cagttcagcg
     2041 gtggtgcgct ggaaatcttg aagcgcacaa tgaacagctt gagccgagag cgcgccggtc
     2101 aagctgcagc cgggttagca tactatgcct ttttctccct gttcccgctc ttgctactgc
     2161 tcgtagttgg aagcagcttc ttcttcgaga gcgatcaggc ctcgcagcag gtggtcgaat
     2221 ttattagcca ggcactgcct gtatccacaa ccctcctgga gagaaatatc gctcaggtgc
     2281 tggaattgcg cggcccggtc agcgccatcg gcttgatcag cctgttatgg tctgcaacta
     2341 gcgtgttttc ggca
//
LOCUS       DBNHOH010000564         2007 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_674556, whole genome shotgun sequence.
ACCESSION   DBNHOH010000564 DBNHOH010000000
VERSION     DBNHOH010000564.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_674556"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2004)
                     /locus_tag="AC16V7_09800"
     CDS             complement(<1..2004)
                     /locus_tag="AC16V7_09800"
                     /inference="COORDINATES: protein motif:HMM:NF012677.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257452.1"
                     /translation="MSHGSPVNRSGKYYNAVHYTVDPHTEQINYDAVEALAQQHKPKF
                     LIAGYSSYPWAVDWARFKEIAKSVGAYLFADIAHVAGLVAAGAYPSPLGYADVITFTT
                     HKSLYGPRGACILTTEPSLARKIDRAVFPGEQGGPHVNVFAAMALAFKLAKTEQFKQL
                     QAQVVNNCIKLTKRMEERGLRIPFGGTNTHLTNVDCSTIKSPDGTTLSGDQTARILDI
                     AGIVVNRNTIPGDPSAAAPSGIRLGTPWVTQRGLDEADMVALADIIADLMLAMTPFGL
                     GGRKGEARRAKVDFTVLEEAKIKVRSLAEKAGIDFDPIQHGYPHFYYLDDKPVSDGWV
                     AFDLVGERVRQFLDYAASCDVDEIKPGESAATCIFTPTGTVEGALTRDLDGAFRLSVP
                     SWQGGLAAAWLRDLSDGYVGFDDDLSRKLSGPVIIRQSSAAPVEKSCLEEPSLSPSRG
                     TGKPYFIGMPAGTGQALPKFEWQEQEAALRRTPIYEVHKKLGAKLIPFAGWEMPVWYT
                     SVVEEHLATRQAAGLFDVAHMGVYQVEGPDAAVFLDSVCGNDISGLGVGESCYTHFLT
                     PDADVIDDTLVYHRAVDKFLVVVNASNDDKDWAWLNAVKNGSVCVDKSRPWTKAYGRG
                     AVLRNLRDPKEGADMRVDVALQGPKSRDVLLAMGCDAETRRRIL"
BASE COUNT          455 a          609 c          508 g          435 t
ORIGIN      
        1 caagatacgc cggcgtgttt ccgcatcaca acccatagcc agtagcacat cgcgggactt
       61 cggtccttgc aatgcgacgt ctacacgcat gtccgcacct tcttttgggt ctcgcaggtt
      121 gcgcaaaacg gctccccgac cataagcttt cgtccacggg cgagatttat ccacacaaac
      181 gctgccattc ttcaccgcgt tgagccaggc ccagtccttg tcatcgttgg aagcgttgac
      241 caccaccagg aatttatcca ctgcacgatg gtataccagc gtatcatcga ttacatcagc
      301 gtctggagtg agaaaatgcg tataacagga ctcaccaacc cctaacccgc tgatatcgtt
      361 accacacacg ctatctaaaa agaccgctgc atctggacct tccacctggt agacccccat
      421 atgcgctaca tcgaacaaac cggcggcttg cctcgtcgcc aggtgctcct caacgaccga
      481 ggtgtaccac acgggcattt cccagcctgc aaaggggatt aacttggcac ctagcttttt
      541 gtgcacctca tagattggtg tgcgacgtaa ggcagcctcc tgttcttgcc actcgaattt
      601 gggcagcgct tgccctgtcc cggctggcat cccaatgaaa tagggtttgc ctgtaccgcg
      661 cgaaggagag aggctaggct cctccaaaca cgatttctct acaggcgctg cactagattg
      721 ccgtatgatc accgggccgg acagtttacg cgatagatca tcatcaaatc ccacataccc
      781 atccgacaaa tctctgagcc acgcagccgc caagccccct tgccaggagg gcacgctcag
      841 acggaacgca ccgtccaagt cgcgggtgag agctccttca acggtccccg tgggtgtaaa
      901 tatgcaagtc gcagcgctct ctcctggctt aatttcatcc acatcacaag atgctgcata
      961 atccaaaaac tgccgtacgc gctccccaac caaatcgaac gctacccaac catcggagac
     1021 gggtttgtca tccaggtagt agaagtgcgg gtaaccatgc tggattggat caaagtcgat
     1081 gccggccttc tccgccaaac tgcgcacctt gattttagct tcttcaagga cagtgaaatc
     1141 taccttggct cgacgggctt cgcccttgcg tccccccagc ccaaagggcg tcatcgccaa
     1201 catcaggtcg gcgatgatgt ccgccaatgc gaccatatca gcttcatcga gtccgcgctg
     1261 ggtcacccaa ggcgtaccca gacggatgcc tgacggagcc gcagccgatg gatcacctgg
     1321 gatcgtattg cggttcacca caatacccgc gatatccagg atccgcgcgg tttgatcgcc
     1381 ggagagagtc gtcccatcag ggcttttgat ggtgctgcaa tccacattgg tcaggtgggt
     1441 gttcgtccca ccaaatggga tgcgcagacc gcgctcctcc atgcgtttgg tgagcttgat
     1501 gcagttattg accacctgcg cttgcaattg cttgaattgc tcagttttcg ccagcttgaa
     1561 tgccagagcc atagcggcga acacgttaac atgcggacca ccttgttcac caggaaatac
     1621 tgcccggtca atcttgcgcg ccaaggaagg ctcagtggtc aagatacaag ctccccgtgg
     1681 gccgtacagc gatttgtgcg tggtgaaggt gatcacatcc gcgtagccaa gcggagatgg
     1741 atatgcacct gccgccacca gaccagctac atgggcgatg tcggcaaaca ggtacgcgcc
     1801 aaccgatttg gcgatctctt taaagcgagc ccaatcgacc gcccagggat acgacgagta
     1861 ccctgcgata aggaacttgg gcttatgctg ctgtgctagc gcctcaactg cgtcgtaatt
     1921 aatctgctcg gtgtgcggat caaccgtgta atgtaccgcg ttgtaatact tgccagatcg
     1981 gttcactggc gatccatgcg acaggtg
//
LOCUS       DBNHOH010000565         4573 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1379644, whole genome shotgun sequence.
ACCESSION   DBNHOH010000565 DBNHOH010000000
VERSION     DBNHOH010000565.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4573)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1379644"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="AC16V7_09805"
     CDS             <1..583
                     /locus_tag="AC16V7_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257453.1"
                     /translation="GASSDGVREPISPYILACNFWLLTANPSDPSANQAWFQPDDYHL
                     PIVTALNKLVRPGDAQQPDQPTPAHQPESEPDLHPPSSTLEDTHPSPVEPKIISAQPQ
                     DEHPIAHYLLLPKYDWGASEWHLDAARPFILKHNPTVGYSPAEAALAARITVVGGEQV
                     FPEELIDEMRQRGCQVTRITGDGTEIASKLAST"
     gene            609..1811
                     /gene="ftsZ"
                     /locus_tag="AC16V7_09810"
     CDS             609..1811
                     /gene="ftsZ"
                     /locus_tag="AC16V7_09810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR6257454.1"
                     /translation="MSTLPYPSEFESQFADIRKPVLKVIGLGGGGGNAVTRMMELGLK
                     DVEFIVANTDHQVLEKNPAPVKIQLGPRVTRGLGAGGNPSIGRAATEESCREIASALQ
                     GADMVFLTAGMGGGTGTGAIPVVAEIARSLGAVTIAIVTTPFSFEMGRRQRNASEGLD
                     LLRKYTNTLIAIPNDRLLFIAPRNLPIETAFRLADDVLRQAVQGITELITEPGMINVD
                     FAHVRRLIQLGGGAMMSIGQGEGEHKTRKALEQALHHPLLETIALENAAGIIANFTGG
                     DDLTLFEVQDALANLQEQTSNRTEIVLGVIQDERMEDRVQITLIVTGLGAPTLEETLF
                     KVAAPSKAEAPKVPQQAALASDPKPMPIPTEKTEPIPQVTSGISPSNLDLPAFLRRRA
                     RLAGLENG"
     gene            1816..2070
                     /locus_tag="AC16V7_09815"
     CDS             1816..2070
                     /locus_tag="AC16V7_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257455.1"
                     /translation="MDMHTLRAVIAQIHKRYPEFAGCNPKVRLQNAPQPKSLLITPTY
                     LLTFQGTARANSATGEKAIPRWMRVVISEKGKILKVTTSR"
     gene            2383..3537
                     /locus_tag="AC16V7_09820"
     CDS             2383..3537
                     /locus_tag="AC16V7_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257456.1"
                     /translation="MTYKIKPLLLKITLVSLFIGVLASYKVGVSEPARAARGLPNSPE
                     FGYGARLDVWGQEVELALNAAAGVGIDWIGVDFDWSRHWPDANHPINLEPLDKALAIA
                     QKQDLNVLLSISHPPAWVLGTDGPDVNQTAGLVVMLANRYPQNLMAIELFPQANTVAG
                     WGASPNPGAYSQLLLASQKALNAGARPVTLVAAGLSPLPAQPAVGDMDDLVYLSELYQ
                     AGAAANMPILSLRLSTVVGEAMASPNQDTRQVLRHYEAVRQVMLQHDHAHGLIWVTGF
                     SWPEESLDSPSDQQIRWLNQAFQLMKSQLYIGVAFFESLNPPQDDKPSASPTQSLIMN
                     DAQGTHMHPALVALGQIITLNQSGHASFQVFLYKKITTGPAKSLWKRRQP"
     gene            3516..4436
                     /locus_tag="AC16V7_09825"
     CDS             3516..4436
                     /locus_tag="AC16V7_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257457.1"
                     /translation="METTATMSLKRPLFFFALFCGFLLVGLSVGRQIVSWRARNLPQL
                     LARSNQLEKQSLPRENTPDLAAQTDHQVSQPPPLPAIPPSQAPSNAIIENGEPLPRQN
                     NILLIGVNNLDVAYPRLESTWLILYMTQMPHLTLMPIYPENPSQDENAEPPGTDLAKL
                     FQSDPKGLPGPDFFTELKKRGLWWSGYIIVDRQALNEVIDLVGRLAEQSGEGELSPEM
                     GILESVPSAWNNPQRAYEGQIQVAQRLCSAAAHITVEHINEIDDIFLLFTDDILSDIN
                     REGAAAELVGMLVQGSGITCEFPTLGNESP"
BASE COUNT         1127 a         1268 c         1195 g          983 t
ORIGIN      
        1 gggtgcatca tctgacggcg taagggaacc gatatcaccg tatatcctcg catgcaattt
       61 ttggctgctc actgccaatc cttctgatcc ttcagctaac caggcatggt ttcagccaga
      121 cgattatcac cttcccattg tgacggcgtt gaacaaactg gtgagacctg gggacgctca
      181 gcaaccagac cagcccacac cagctcacca accggagagc gagcctgatc tccacccgcc
      241 cagcagcacg ctggaagaca cgcatccatc acctgttgag cccaaaatta ttagcgcaca
      301 accccaagat gagcatccta ttgctcacta tttattgctg cccaagtatg attggggcgc
      361 ttctgaatgg catctcgatg cggcgcgccc atttatcctg aaacataatc caacagtggg
      421 ttattcacct gccgaggctg cgttagcggc gcggattacc gttgtggggg gagagcaagt
      481 attccctgaa gagctgattg atgagatgcg ccagcgaggc tgccaggtga cacgaatcac
      541 aggggatggc acggaaattg catcaaaact ggcatccact taaatctgta ccgaaagggg
      601 catttcccat gagtaccctc ccatatccat ccgaatttga aagccaattt gcagatatcc
      661 gaaaacccgt cttaaaggta ataggcctgg gtggcggagg gggtaacgcg gttacccgca
      721 tgatggagct tggcctgaaa gacgtcgaat ttatcgttgc caacacagat caccaggtac
      781 ttgagaaaaa tccggctcca gtcaaaatcc agcttggccc gcgagttacg cggggattag
      841 gggcaggtgg caaccccagt attggccgcg ccgccaccga agaaagctgt cgagagatcg
      901 cctccgcttt gcaaggggcg gatatggtat tcctgaccgc cggtatggga ggtggtacag
      961 gtactggcgc gatcccggta gtggcagaga tagctcgctc gcttggggcg gtaacaatcg
     1021 ccattgtcac cacgcccttt tccttcgaga tgggccgccg ccagcgcaac gccagcgaag
     1081 gcttggattt actccgcaag tacacgaata cattgatcgc catcccgaac gatcggttat
     1141 tgttcatcgc tccacgcaac ttgcctattg agaccgcctt ccgcctggca gatgatgtac
     1201 tccgccaggc cgttcaggga atcaccgagc tgatcaccga gccgggcatg atcaatgtgg
     1261 atttcgctca tgtgcgacgc ctgattcaac ttggtggagg cgccatgatg tccatcggac
     1321 aaggagaagg cgaacataag acccgcaagg cgctggaaca agccctgcac catccattgc
     1381 tagagaccat cgcgctggag aacgcagccg gtatcattgc caatttcacc gggggcgatg
     1441 acctgacgct cttcgaggtc caagacgcat tggctaatct acaagagcaa acctcaaatc
     1501 gaactgaaat cgtgctcggc gtaattcaag acgagcgcat ggaagacagg gtgcagatta
     1561 ccctgatcgt caccggtctg ggtgctccca ctctggagga aaccctattc aaagtcgctg
     1621 caccctccaa agctgaagct cccaaagtac cccaacaggc tgccctggct tcagacccca
     1681 aaccaatgcc tattccaacg gaaaagaccg agcccatccc acaagtgaca tcggggatct
     1741 cccccagcaa cctggacttg ccagccttcc tgcgccgccg agctcgcctg gctgggttag
     1801 agaatggata acgccatgga catgcatact ctccgagcgg taattgccca aatccacaaa
     1861 cgctatccgg aattcgccgg gtgtaacccg aaggtgcgtt tgcaaaatgc cccgcagccc
     1921 aagtcgctgt tgatcacgcc aacttattta ctgacttttc aaggcacggc acgcgcgaat
     1981 tccgccacag gcgagaaggc catcccgcgc tggatgcgcg tggtcattag cgaaaaagga
     2041 aagatactaa aggtcaccac atcccggtga gcgagtcatc ctggagaaag cttgaactta
     2101 tgagcatccg tactattact tctagaattg agattgcctt ttggtctgtc gtcgtttcat
     2161 tgctcagcga ctctgagctc attcagggat tcgtccaaac atgctacatc ggtttcacgt
     2221 ctggacaata tacccgaaca ctgaaaaagg cgcttattct ctctgcagca ggattcttga
     2281 ttggaatcgt actggggtta gcaggtttgt gaaacgcgga gtggaattca actaaattcg
     2341 cgccagtgta aattagaaaa cccctacaga tgatatccga gcatgaccta caagattaaa
     2401 ccccttctcc tcaagatcac tttggtttct ctattcatcg gggtgctagc aagctacaaa
     2461 gtgggggttt cagagccagc gcgcgcagcc agaggcttgc ccaattcccc tgaatttggc
     2521 tacggggcgc gtctagatgt gtggggccag gaagtggaac tggcgctaaa cgctgccgcc
     2581 ggggtgggta tcgattggat cggcgtagac ttcgattggt ctcgccactg gccggatgcc
     2641 aaccacccga tcaatctaga gcctttggac aaggcattgg caatagccca aaaacaggat
     2701 cttaacgtac tgctatcaat ttcacaccca cctgcctggg tgttgggcac tgatggaccc
     2761 gatgttaatc aaaccgcggg gctggtagtc atgctggcga accgttatcc gcaaaacctg
     2821 atggcgattg aactcttccc acaagccaat acggtagctg gatggggcgc gtcacccaac
     2881 cccggcgcat actcccagct tcttctggcc agtcaaaaag cattgaatgc tggcgctcgc
     2941 ccagtaacgc tggtagctgc tggtttgagc cctctcccgg cacagccagc agtgggtgat
     3001 atggatgatc tggtttattt gagcgagctc tatcaggcag gtgcggctgc aaatatgccc
     3061 atcctgagct tgcgtctgag cacagtcgta ggtgaggcga tggcatcgcc caaccaagat
     3121 acgcgacagg tgctacgcca ctacgaagca gtccgacagg ttatgctgca gcacgatcat
     3181 gctcacggct tgatatgggt tacgggtttc tcctggcctg aagagagttt ggacagccct
     3241 tcagaccaac aaatccgatg gctcaaccag gcattccaat tgatgaaatc gcagctatac
     3301 atcggagtag cctttttcga gagcctcaac ccgccacagg atgacaagcc ctcagcgtcc
     3361 cccactcaaa gcctgatcat gaatgatgcg caaggcacgc atatgcatcc ggctctggtc
     3421 gcccttggac aaatcatcac gctcaatcag agcgggcatg cttccttcca ggtattcctg
     3481 tacaaaaaga tcaccaccgg tccggcaaag agcttatgga aacgacggca accatgagct
     3541 tgaagcgccc gctttttttc ttcgcactat tctgcggatt cctgctggtt ggtctgtcgg
     3601 taggcaggca aatcgtgtct tggagagcga ggaatctacc acagctccta gctcgttcta
     3661 atcagttaga aaaacaatcc ttgccgaggg agaatacgcc agacctcgca gcgcagaccg
     3721 atcatcaagt atctcaaccc cctccgctcc ctgccatccc cccttcgcag gcaccctcaa
     3781 acgccatcat cgagaacggc gaaccgcttc cacgccagaa caatatcctg ctaatcggcg
     3841 taaacaactt ggacgttgca tatccacgcc tagaaagcac ctggctaatc ctttatatga
     3901 cccagatgcc tcacctgact ttaatgccga tctatccgga aaatccctcc caagatgaga
     3961 acgctgaacc gccaggcacc gatcttgcca aacttttcca atctgatccc aagggtttac
     4021 ccggtccgga tttcttcacc gagctcaaaa aaaggggact gtggtggtct ggttatatta
     4081 tcgtcgatcg acaggctctg aacgaggtta tcgatctggt tggcaggctg gccgagcaga
     4141 gcggagaggg tgaactctct cctgagatgg gaattttgga aagcgtaccc tccgcctgga
     4201 ataatccgca gcgggcctat gaaggacaga ttcaagttgc ccagcggctt tgcagcgcag
     4261 ctgcacacat cactgtggag cacattaacg aaatcgatga tatcttcctc ttgtttacag
     4321 acgatatctt gagtgatatt aaccgggagg gggcagctgc ggagctggtt ggcatgctgg
     4381 tgcagggcag cgggatcacc tgtgagtttc ccacactggg taacgaaagc ccataatcat
     4441 cgagctaatg cagtaaaaaa caaaagggta cggtgtaggg atttttatac gtggcaattc
     4501 cgaccgccct aataatcata ggacccacgc agttgaagag gaaaggaggg ggggtggggg
     4561 gggggggggg ggg
//
LOCUS       DBNHOH010000566         1791 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1187338, whole genome shotgun sequence.
ACCESSION   DBNHOH010000566 DBNHOH010000000
VERSION     DBNHOH010000566.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1187338"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="AC16V7_09830"
     CDS             <1..411
                     /locus_tag="AC16V7_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6257458.1"
                     /translation="NRLWFYFAFRFLLVHTLGLPTQARLVAKRIFPHENQAAFRENFL
                     QQINQADPKGYRAVMRALAFFDVTERLAEINIPTLVVSGEEDTTIALTTQQHLSHWIP
                     NTHHVIIPKGGHAITVEQPGIFNETMISFLQKLS"
     gene            657..1574
                     /locus_tag="AC16V7_09835"
     CDS             657..1574
                     /locus_tag="AC16V7_09835"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6257459.1"
                     /translation="MTSKVVKKRGQYILAFDGSEHARAAVSFMQDFIIQQGEKNKSTV
                     VLLAVFTPRQIGDLTPLEQALDQTHIFFKDKGIRSKPELVLGYPGEKILEYAEELNPK
                     MIVVGAKGLRATLGILLGGVAQEVVESAQCPVMVIRAPYQVFQRILLVADGSTYSQKA
                     IEYVAKFHLTPLASIDIINVLPPIPIKPVPDFITHSWPAGPEVIPQFTPEMSEEESAF
                     LREEEKEGQKILDGAQSLLQKKGKPVQINQVLVRGDAASEIIQYAKDNKIDLIVTGSH
                     GMGAIRSILLGSVTRKLVHYANSSVLVVK"
BASE COUNT          509 a          417 c          393 g          472 t
ORIGIN      
        1 aacaggctgt ggttttactt tgcgtttcga tttctgctgg tgcacacact cggtttgcca
       61 acacaagccc gcttggtcgc gaaacgcatt tttccacacg aaaaccaggc agcgtttcgt
      121 gagaactttt tacagcagat aaaccaagct gatcctaagg gctatcgcgc agtaatgaga
      181 gcgctggctt tttttgatgt taccgagcgt cttgctgaaa ttaatattcc tacgctggtt
      241 gtctctgggg aggaagatac aacaattgca ctaactaccc agcagcatct atctcattgg
      301 atacccaata cacaccatgt gatcatccca aaaggggggc atgctatcac ggttgaacaa
      361 ccgggcatat ttaatgaaac catgatctct tttttgcaaa aactgagttg acctgggtgt
      421 cttctacaca aataccaggc gagaaggtcg gctcaagctt caactaaaca tcctcttgaa
      481 ctaccaatac atctatgcta tagtcaatat ggagcgaaca gccgatcttc gccggacggc
      541 aatccagagc aattaggcat catcaatcaa catcatcaag acctgtctgg ttagatgatc
      601 tcagggtcat tccaggcagg tctattttta taagaaaatt taataggagt gcagctatga
      661 cctccaaggt tgtcaaaaaa cgtggccaat atattctagc ttttgatggc tctgaacatg
      721 cccgcgctgc agtctcattt atgcaagatt tcatcataca gcaaggagaa aaaaataaaa
      781 gcacggtcgt gttgttggcg gtttttactc ctcgtcagat cggagacttg acgccgctcg
      841 aacaggcact tgaccagacc cacatttttt tcaaagacaa aggcattcgt tctaaacccg
      901 aactggttct tggatatcct ggagaaaaaa tccttgaata cgctgaagag ctaaatccga
      961 agatgatcgt cgtcggagcg aaaggtctgc gtgccacgct tgggatttta ttgggcggtg
     1021 tagcccaaga agttgtcgag tccgcccagt gcccggtaat ggttatccgg gcgccatacc
     1081 aggtatttca acgcatattg ctggttgctg acggatccac ttatagtcaa aaagccatag
     1141 aatacgtggc caaattccat ctcacgccat tggctagtat tgatattatc aatgtgctac
     1201 cacctatccc cataaagcca gtcccagatt ttataaccca ttcctggccg gcaggtcctg
     1261 aagttatccc ccaattcacc ccagagatgt ctgaagaaga atcggctttt ctgcgcgagg
     1321 aagagaagga aggtcaaaaa atattggatg gagctcaaag tctattgcag aagaaaggga
     1381 agccggtgca aattaatcaa gttcttgtgc gcggtgatgc tgccagcgag attatccaat
     1441 atgcaaaaga caataagatt gacctgattg ttacgggttc gcacggcatg ggcgcaatca
     1501 ggagtatatt actaggcagc gtcacccgca agctggtaca ctacgcgaat agctctgtat
     1561 tggtcgtaaa gtaatcctgc ctcccaattt catcctgtaa actgccatta cgtttacaga
     1621 atgcattatc gtgcgttgat aagattgctc cccgaaattc ttaaaattaa tatggactat
     1681 aatgggtaca tatctctctt taggtgaact ccatgaaatc acatttctcc agcttgaata
     1741 ctataacaac cgagccttat aatgccgaaa ctccccatgc ggttttgatg a
//
LOCUS       DBNHOH010000567        10553 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1187383, whole genome shotgun sequence.
ACCESSION   DBNHOH010000567 DBNHOH010000000
VERSION     DBNHOH010000567.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10553)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10553)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10553
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1187383"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2624)
                     /locus_tag="AC16V7_09840"
     CDS             complement(<1..2624)
                     /locus_tag="AC16V7_09840"
                     /inference="COORDINATES: protein motif:HMM:NF017539.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6257460.1"
                     /translation="MEDQNSPVIFSEWLKRRRKALDMTQDELAKRAGCSVGALRKIES
                     GDRRPSKQLADLLAKALEIPEEDLQTFIRVARGELNLERLRPPALESLAPLPDISSLR
                     QTQPSASLDASTAPPSHRIPLQATPLIGRDNELAALERLFNDPQCRLLTLTGIGGIGK
                     TRLAIEFATQRLSVFPGGVFYIPLTPVNSPEKIVPAIADVLNIEFFGPSDLIEQFFYY
                     ISSHINQAVLFVLDNLEHLLVHEPTQDDKPGVVELVSEILQRLPNVKILGTSRERLNI
                     LGEWVYELHGLSVPQTNFTGNLEAYDSVALFVKSAQRIRTEFQVSAEDQLSLVRICQL
                     VEGAPLAIELAAAWVGILSCQEIAQEIKSNMDFLTTSMRDIPERHRSIRATFDHSWKL
                     LSDEERWALCQLSVFHGGFDRYAANQIAGASLPLLASLSDKSLVRRTASGRYDLHEVI
                     RQYSLSHLNEHPSKLETYQRHCEYYLKLLQEHETSLKDASQQEVIRQLTDEIDNIRAA
                     WAWAIQHKKYAELDQAGRGFGWYYEITGLYREGIEQLGSLVQALEAEPQGDKWHRVLG
                     LMLVHQALLHFRVGEFSRARNLYAQSIRILRPIGDQILLADGLIYLGILLHLMGEYEQ
                     ARSSVEEGLFFAKESNARWYEAYAIYNLGYIDSLMGRYEEGYEQMLVGLGIWRSLGDP
                     HYIALGLNFLVPTLNKLGRCEEAKTFMRESIALCEQSKNLWGLGTAYRYFGLACLAGG
                     QFTEAQTHIRKSLEIYGEFVVGYDIARSLAYLGDVAVMLGDLTTAKKDYLEALRISIE
                     EKVIPIALDALLGLGDLQAQLGKTEDALVLCYHILNHPACEDDTKSRVEKLRATLEPK
                     LNSKQVMAARTLATEKTF"
     gene            2753..3277
                     /locus_tag="AC16V7_09845"
     CDS             2753..3277
                     /locus_tag="AC16V7_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257461.1"
                     /translation="MLVPYLIVLRFFHVVASVCWAGGGFIFFLFVEPTAKALAPTGMQ
                     FVQYMVTKRRFSIFMVVSSTLTVLSGALLIWQLTGGQLIGYMKTGPGLGFSLGSIVGI
                     VVYFVGMFGINPRAIRLSKIGDEIQAAGGPPTPEQAAELHKLDKEMSALSLADFVLVA
                     LSLGLMATARYWVF"
     gene            3447..4442
                     /locus_tag="AC16V7_09850"
     CDS             3447..4442
                     /locus_tag="AC16V7_09850"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR6257462.1"
                     /translation="MSQKVYVDYQKSTSPFLAGGDLINIEANKLQGVSTVDFHLTSRC
                     SQACPYCWGPRRFRNSVAKENAQSIIVRIKHLGVRRIVFTGGDPLQHPDVLDLIRYAK
                     DMGLETALSTTGDLVTPEVLQGLAPNLDLISLPLDGSTEEINAKTKHRGHYSAVMRSL
                     EWLRSYPGIDVKVCTPVTRHNLSDIPAIAQLVEGYSLTTQARVFYNVFQAFPRAMFSV
                     NWERLVVTDEEFAALAHQIGKGKNIRINFLDHDILDKLYVMVFPDGSMVIPKGADYLN
                     FGPFLEVKDFNSALKASQFDSAKHVRHSLGWGKAATPIRQALNVSLVDSFNILKV"
     gene            4447..5472
                     /locus_tag="AC16V7_09855"
     CDS             4447..5472
                     /locus_tag="AC16V7_09855"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HIR6257463.1"
                     /translation="MKTLTTKEDVNSLLHASTAKAALGAAIKTGLLWMLAERPMTAEQ
                     VAQALNIPGKRGYYWLQYLQSFGVLESVPQGYVPSSVVRTAILETYSRESWQHLVIDE
                     QEKDACVHGLPQLISEPGSLWTVQGLEEPKNYVDRMRVNLERAREFTIMLFEVHQNLA
                     NLVADLLDLTGVERMMDLGGNSGVVSMALLRKYPALTCTVLDIENVCIAGREIAEREG
                     FSDRITYQAADFEDDEFPSGFDLVLQCDVAQYGTELINKLYRSLKPGGRLIYVDHFSP
                     AENLAPTTRLEWTFLDSLHDPNFSFPTLDEVKSRLAQTGFEVSDELHTLGRGLIVLQA
                     WKRDVAH"
     gene            5506..6384
                     /locus_tag="AC16V7_09860"
     CDS             5506..6384
                     /locus_tag="AC16V7_09860"
                     /inference="COORDINATES: protein motif:HMM:NF012995.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HIR6257464.1"
                     /translation="MAYPNLSDTLVKDILDYLECPKKAPTLRYLNHLIKAYLRKVPWE
                     SVSRIVKRHATLETEDCPRWPEEFWQETMQLGFGGTCYESSLAFYSLLMALGYEGYLT
                     VNDMGASRGCHAAIVLLLNKHKYLVDITMPIHVAVRIDPQKIVRRNTAFQNYTIRPVQ
                     ENKFEVERSHHPNRNAFTMIDIPVSLQDYQEIVKNDYRETGFFLRSVVMVRVMGDKTW
                     RFFSDHKPYKLESFDRAGKREIFLEPETLPHILAELFQMPEDQISAALSWISTPSIMP
                     RNEIISLPMVLEGSAQ"
     gene            6381..6758
                     /locus_tag="AC16V7_09865"
     CDS             6381..6758
                     /locus_tag="AC16V7_09865"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR6257465.1"
                     /translation="MKPLTLDLFNSWLETYGKASRENDLQASADLFSLDAKYYETPFD
                     EPMIGRDAIYQYWSQGAQRFKDKESSYEIISMEDNLGIARWQSQFTAINSGKRLRLDC
                     LFLVEFDENGLCSVFREWWHISE"
     gene            6846..7871
                     /locus_tag="AC16V7_09870"
     CDS             6846..7871
                     /locus_tag="AC16V7_09870"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HIR6257466.1"
                     /translation="MDKTKQQITRIAIVGTGHVGATCAYALQLLGHASEIVLINAHRE
                     RAEGEAMDISHGSLFARPVRIWAGDFSDCRDADIVVLTAGASQKPGETRLDLLRRNAA
                     IYQNILPQVVQYNRNAILIVVANPVDVLTYITLQITQLPARQVIGSGTILDTSRFRCL
                     LSQHFGIDPRSIHAYILGEHGDSQVPVWSLANIAGMRLDEYARLNNKPLDDYSRHEIA
                     QNTRQAAYEIIRRKGATYYAIAAGVVRIIEAIVRDENSVLTLSSLIQGKYGLNDVCLS
                     LPSIVNRQGILETLELPLSPEEQTGLEKSARVLREAIDSLQEYTSPNLVFNIPSRYSV
                     GCTYENR"
     gene            7858..8793
                     /locus_tag="AC16V7_09875"
     CDS             7858..8793
                     /locus_tag="AC16V7_09875"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6257467.1"
                     /translation="MKIVEYDDVDPLKALCVNQLALEFALSPEKVAHIRQTDPRPFPC
                     LAIYAVEDGVVLGQIGIFRLPMISTEGREDVGGVWAVSTHPQYAGHGVASRLLEEAHT
                     RMRDAGLRFSTLGTDRYRVAYNLYRQHGYEETNVWATALARWETAHQPTRLRAQLPGP
                     EGHDIVEQVYKNIASDYLGFAWRHTPFVRLHDTVSLENIWILWEHQQPVGYALVQVDK
                     AMLKITNLLLRDGINAAEAIAAVVVKTKTPYIQVRVSRPSEVASLQRAGYQVAHPTWD
                     AFLVKSLVPEVTADDARRLFGIGTDRFLISWLDVT"
     gene            complement(9183..9962)
                     /locus_tag="AC16V7_09880"
     CDS             complement(9183..9962)
                     /locus_tag="AC16V7_09880"
                     /inference="COORDINATES: protein motif:HMM:NF020419.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HIR6257468.1"
                     /translation="MLDLQQIKNEYSEPLQGYERAILREYLQYKILQAIFESKQASML
                     SFLGGTALRIVHGNSRFSEDIDLDNFGLSSQAFGELIQKVKLFLGLEGFLVETSMVAK
                     GAFHCDLRFPELLYKQGLSPLHQEKILIQLDTIAQGYTYVPEVKILNKFDVFTEIRVT
                     PLNVLLSQKIYTAINRKRPKGRDFYDITYLFSKTRPDFGFIKQKMGITSPQSLREELL
                     LKIGDIDFDALAGDVAPFLITKEQVMRVVKFREFWKQVDLD"
     gene            complement(9955..>10553)
                     /locus_tag="AC16V7_09885"
     CDS             complement(9955..>10553)
                     /locus_tag="AC16V7_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HIR6257469.1"
                     /translation="SRIRALPTFNLNDVRKLDPGFHRQQLNYWHNQGYIKSLAGGYYI
                     LADRVMDEMVLFMVANKIYEPSYVSLESALAYYEIIPETVLGVTSISSRKTKQYESAW
                     GVFSYRSVKPQTMIGYQVIENTPAIKFKIAYLEKALLDYLYLNSEIQSLADFEELRWN
                     RTQLRGLLDHSIFAKYVKLFDKRALKNRVNQFLEYLNA"
BASE COUNT         2678 a         2551 c         2620 g         2704 t
ORIGIN      
        1 tcaaacgttt tctcggttgc caaagtgcgc gccgccatga cttgtttgga gttcaatttg
       61 ggctccaagg ttgcgcgtag tttttccacg cggctttttg tatcgtcttc gcaagcgggg
      121 tgattcagga tatggtagca aagtacgagg gcatcttcgg ttttgccaag ttgagcttgc
      181 aggtcgccca aacccaataa tgcatccaat gcgattggaa tcactttttc ttctatagat
      241 atgcgtaggg cttccagata atcttttttg gccgtggtta aatctccaag catcacggcg
      301 acatccccca gataggcgag agagcgggca atgtcatagc ccacgacgaa ctcaccgtaa
      361 atctccaggc ttttgcgaat atgagtttgc gcctccgtga attgacctcc tgcgagacac
      421 gccaatccaa agtagcgata tgcagtgccc aaaccccaaa gattttttga ttgctcgcaa
      481 agtgcgatgc tttcccgcat gaaggttttg gcttcttcac agcggcctaa tttatttagg
      541 gtcggaacaa ggaagtttag ccccagggcg atgtaatgcg ggtcgccgag tgaccgccat
      601 atgcccaggc caaccaacat ttgctcatac ccctcttcat aacgtcccat caggctatcg
      661 atgtagccaa gattgtagat agcgtatgct tcataccagc gtgcattgct ctccttggca
      721 aaaaataatc cttcctctac cgatgatctt gcctgttcat attcacccat taaatgaagg
      781 aggataccta aataaatcag cccgtcggcc aacaaaatct ggtcgccaat tgggcgaagg
      841 attctgatgc tttgtgcata aagatttcgg gcacgactga attcgccgac tcggaaatgt
      901 agcaatgcct ggtggacaag catgagcccc aaaaccctgt gccatttatc accttgtggc
      961 tcagcctcca atgcctgcac gagcgaacca agctgttcga tcccctcacg atataagcct
     1021 gtgatttcgt agtaccagcc gaagccccgc ccagcctgat cgagctcggc gtattttttg
     1081 tgttgaattg cccatgccca agcagcgcgg atgttgtcta tctcatcggt caattgtcga
     1141 atgacttctt gctgagaggc gtcctttaac gaggtttcat gctcctgtaa caatttcagg
     1201 taatattcgc aatgccgctg gtaagtttca agcttgctcg gatgctcatt tagatgggac
     1261 aaggaatatt ggcggattac ctcatgcaga tcgtaacgtc cactcgcggt gcgccgcacg
     1321 agcgatttgt cgctcaggga agccagaagg ggcagcgatg ctcctgcaat ctggtttgcc
     1381 gcataacgat cgaagcctcc atgaaaaact gatagctggc aaagagccca acgttcttca
     1441 tctgacaata atttccatga atggtcaaag gtggctctga ttgagcggtg gcgctcggga
     1501 atatcgcgca tggaggtggt caggaaatcc atattggatt taatctcttg cgcgatctct
     1561 tgacaggaaa gtatgcccac ccatgccgcc gccagttcaa tagctagggg agctccttcc
     1621 accaattgac aaattcgaac cagagataat tggtcttctg cggatacttg aaattctgtt
     1681 cttatccgct gagcgctttt cacaaatagc gcgacagagt cgtacgcttc caagtttccc
     1741 gtaaaattgg tttggggtac cgagagtcca tggagttcat atacccactc tccaaggata
     1801 ttcaagcgct cgcgagacgt tcccaggatt ttgacatttg gcaatcgctg aagtatctct
     1861 gaaaccaatt caaccacacc aggtttgtca tcttgtgtag gttcgtggac gagtagatgt
     1921 tccaaattat ccaagacaaa aagcactgct tggttgatgt ggcttgatat gtaatagaaa
     1981 aattgttcta ttaggtcgct gggtccgaaa aattcgatat tcagcacatc tgctatggcg
     2041 gggacaattt tctcggggga attcacaggg gtaagcggaa tatagaaaac acccccaggg
     2101 aatactgaaa gcctttgcgt cgcaaattca atcgcaagcc gagttttacc aatgccgcca
     2161 atgcctgtca gcgtgagcag acgacattgt gggtcattga agagcctttc cagcgcggct
     2221 aattcgttat cgcggccaat taaaggcgtg gcttgtaatg gaatgcgatg tgaggggggg
     2281 gcggtactcg catcgagaga cgcgctgggt tgagtttgtc gaaggctaga aatatcaggc
     2341 aaaggtgcca gggattcaag tgcagggggg cgcagccgct cgaggtttaa ctctccgcgc
     2401 gccacgcgta tgaatgtctg cagatcttct tctgggattt caagcgcttt ggccagtaaa
     2461 tccgcgagtt gtttggaagg tcttcggtcg cccgattcaa tttttcgaag agccccaact
     2521 gagcaaccag cacgttttgc taactcgtcc tgggtcatgt cgagtgcttt tcgacgacgc
     2581 ttgagccatt cactgaaaat gacgggtgaa ttttgatctt ccattttaga taaaaattgt
     2641 agcacatttt ggggtagtgc ttggggtatt ggctatcccc aatcaaccca aaactggttg
     2701 tttaataagg tcaacatact taatccacta gcaaatcaaa ttggaggtca acatgttagt
     2761 cccgtatctg atcgttcttc gttttttcca tgtcgtagcc agcgtatgtt gggcgggcgg
     2821 cgggttcata ttcttcttgt tcgtggagcc cactgctaaa gctctagcgc caacagggat
     2881 gcagtttgtc cagtacatgg tcaccaagcg tcgcttcagc attttcatgg ttgtctccag
     2941 cacgttgacc gtgttgtctg gtgcgttgct aatttggcag ttaacaggtg gtcaattgat
     3001 tggctatatg aaaacaggtc ctggtcttgg gttctccctt ggctcgatag ttggcattgt
     3061 ggtctatttt gtcggcatgt ttggaataaa cccccgcgcg atcagactgt ctaaaatcgg
     3121 tgacgagatc caggccgcgg gaggcccgcc cacccccgaa caagctgccg aacttcacaa
     3181 gcttgacaaa gaaatgtcag ccctaagcct ggcggatttt gtgctggttg ctttgtccct
     3241 ggggctcatg gctacagctc gttactgggt gttttagcaa gcgcacgcat cccacagcct
     3301 actagatcgg tcaggcaaat gtggatgcct ggccgatctg agaaaagcct tgacataaat
     3361 aactccctcg tctggaatag taaccacgtt tcttcacggt caatccgaga tattatcaaa
     3421 cggaaaagcc acacaagagg tttcccatgt ctcaaaaagt ttatgtcgat tatcagaaat
     3481 ccacaagtcc atttctcgca ggcggcgatc ttataaacat tgaggcaaac aaactgcagg
     3541 gcgtttcaac cgttgatttt catctcacca gtagatgcag ccaggcatgc ccctactgct
     3601 ggggaccgcg gcgcttcaga aactctgtgg ctaaggaaaa tgcccaaagc atcattgtcc
     3661 gtatcaagca tttaggcgtg cggcggattg tattcacagg cggcgaccct cttcaacatc
     3721 ctgacgtatt ggacttgatc agatatgcca aagacatggg ccttgagacg gcgctctcta
     3781 ccacaggaga ccttgtaacg cctgaagttc tgcaaggtct tgctccaaac cttgatttaa
     3841 tctctctccc attggatggc tccaccgaag aaattaacgc caaaaccaag catcgcgggc
     3901 attactcggc tgtcatgcga tcactcgagt ggcttcgatc gtatcctggc attgatgtca
     3961 aggtatgcac accagtcaca cgccacaatt tgagcgacat tccagctata gcccagctgg
     4021 tcgaagggta ttctcttacc acgcaggcga gggttttcta taacgtcttt caggcattcc
     4081 cgcgtgcgat gttttcggtc aattgggaaa gactggttgt gaccgacgag gagtttgcag
     4141 cgctggcgca tcaaattggc aagggcaaaa acatccgcat caactttctg gatcatgaca
     4201 tactagacaa gctgtacgtc atggttttcc ctgacgggag catggtcatc cccaagggtg
     4261 cagattatct aaattttggg cctttccttg aggtaaagga ctttaacagt gctctgaaag
     4321 ccagccagtt tgactctgcc aaacatgtgc gccactcgct ggggtggggt aaggcggcga
     4381 cgccaatcag gcaggctctg aatgtaagtc tcgttgactc cttcaatatc ctcaaggtgt
     4441 aaaaaaatga aaacactgac tacaaaagaa gatgtaaata gcttgctcca tgcctctact
     4501 gcaaaggcgg cgctgggagc agccatcaaa acaggactgc tgtggatgct tgcagagaga
     4561 cccatgaccg ctgagcaagt ggcacaagcc ttgaatatac ccggcaaacg cggctattac
     4621 tggttgcagt acctgcaatc tttcggagtt cttgaatccg ttccgcaggg atatgtgcca
     4681 tcctctgtgg tgcgcactgc aattctggag acctatagtc gggagagctg gcagcatctg
     4741 gtgatcgatg aacaggaaaa ggatgcctgc gtgcatggtc tgccccaact catcagcgag
     4801 cctggctcac tgtggaccgt tcagggtttg gaagaaccga aaaactatgt cgatagaatg
     4861 cgagtcaacc ttgaaagggc gcgtgaattc acgattatgc tcttcgaggt ccaccaaaac
     4921 ctggcaaacc tggtcgcaga cctgctggat ctaacgggtg tcgaacgcat gatggacttg
     4981 ggtggtaatt caggggtggt ctccatggcg ttgttgcgaa agtatcccgc actcacctgc
     5041 acggttttgg acattgagaa tgtctgtatt gccgggcgtg aaattgccga gagggaggga
     5101 ttttctgacc gcatcaccta tcaggctgct gattttgagg atgacgaatt cccatccggt
     5161 ttcgatctgg tgttgcagtg tgacgtagcc cagtatggta ccgaactgat caataaattg
     5221 taccggtctt tgaaaccggg cggacgattg atctatgtgg atcacttttc ccctgcggaa
     5281 aatctcgcac ccactacaag gctggagtgg acattcctgg attctttgca cgaccccaat
     5341 ttcagtttcc caacactcga tgaggtcaaa tcacgattgg ctcaaacagg ctttgaggtg
     5401 tcagatgaac tccacacact gggcagaggg ctgatagttc tccaggcgtg gaaaagggat
     5461 gtagcacatt gatgttcaat gtggaaaagc aaggagcggc agtcaatggc atacccaaat
     5521 ttgagcgata ccctggtaaa agatatattg gactacctag aatgtccaaa aaaagcgccg
     5581 accttgcgat atttgaacca tctcattaaa gcttatctcc gcaaggttcc gtgggaaagc
     5641 gtctcgcgta tcgtaaaacg tcatgcaacg ctggaaacag aagattgtcc gcgctggcct
     5701 gaagaattct ggcaggaaac catgcagctt ggctttggcg ggacgtgcta cgagtcaagc
     5761 ctggcttttt acagcctgtt aatggcgctg ggctatgaag gttatctgac cgtcaatgac
     5821 atgggagcct cgcgcgggtg tcatgccgcc attgttttat tgctaaacaa gcataaatat
     5881 ctggtggata tcaccatgcc catacacgtt gccgtccgca tcgatccgca aaagatagtg
     5941 aggcggaaca ccgcattcca aaattacacg attcgacccg tgcaggaaaa caaatttgaa
     6001 gtggagcgat cccatcaccc gaatagaaac gccttcacca tgattgatat tcctgtcagt
     6061 ttgcaggact atcaagaaat tgtgaaaaat gattatcggg aaacaggttt ctttctacgc
     6121 agcgttgtca tggtcagggt gatgggcgac aaaacctggc gattctttag cgatcataag
     6181 ccctacaaac tggagagctt cgatcgagca ggtaaaaggg aaatatttct ggagcccgaa
     6241 actctgcctc acatattggc agaattattc caaatgcctg aagatcagat ttctgctgcg
     6301 ctatcatgga tatcaacccc gtcaattatg cctcgtaatg aaatcataag tcttcccatg
     6361 gttctggaag gaagcgcgca atgaaaccac tgacattgga tttgttcaac tcctggcttg
     6421 aaacatatgg caaagccagc agagaaaacg atctgcaagc ctcggctgat ttattctcgc
     6481 ttgatgccaa atattatgaa acgcccttcg atgaacctat gattggacgc gacgcgattt
     6541 accagtattg gagccaaggc gcgcagaggt ttaaagacaa agaatcttct tatgagatta
     6601 tttcgatgga agacaatttg gggattgcac gctggcaatc ccaattcacc gccatcaatt
     6661 ctgggaaacg cctcaggctg gactgtctgt tcctggttga gtttgatgaa aatggacttt
     6721 gtagcgtgtt tcgtgaatgg tggcatatat cggaatagta tgcccatgac cggtagcgag
     6781 acaatttttt tgaattatag aaacacacat caacgctaaa tagtttcggt tttgcagagg
     6841 taaacatgga taaaacaaaa caacaaataa cccgcatagc gattgtcgga acaggccatg
     6901 taggtgcaac atgcgcgtat gcgctgcagc tcttggggca tgcatctgaa atcgtcttga
     6961 tcaacgctca ccgcgaacgc gccgaaggtg aagccatgga tatcagtcat ggatccttgt
     7021 ttgctcggcc ggtgcggata tgggcgggtg atttctctga ttgcagagac gcggatattg
     7081 tggtgttgac ggcgggagct tcgcaaaaac caggtgagac caggttggat ttgctaagac
     7141 gcaatgctgc gatctatcag aatatcctgc ctcaggtggt gcaatataac aggaatgcta
     7201 ttttgatcgt agtggcgaat ccggtcgatg tgcttaccta tattacattg caaataactc
     7261 aattgccggc gcgccaggtg attggctctg ggacgattct ggatacatcc cgcttccgct
     7321 gtttactcag ccagcatttt ggaatcgatc cgcgcagcat tcacgcttac attctcggag
     7381 aacatggcga ctcgcaagtt ccggtatgga gtctcgctaa tattgccggc atgcgattag
     7441 atgaatatgc ccgcttgaat aataagcctc ttgatgatta ttcaaggcat gagatagccc
     7501 aaaacacgcg ccaagcggcg tatgaaatta tccgcagaaa aggcgctacg tattatgcaa
     7561 ttgctgcggg tgtagtaagg attattgagg caatcgtgcg cgatgagaac agtgttctaa
     7621 ccttatcaag cctgattcaa ggcaagtatg gattgaatga tgtttgcctc agcctgccca
     7681 gcattgtgaa tcggcaaggc atcctagaaa cgttggagtt gcccctctct cctgaagaac
     7741 aaacgggtct tgaaaaatca gccagagtcc tgcgcgaggc aattgattcg cttcaggaat
     7801 atacctcacc taaccttgtt tttaatatac cttccaggta ttctgtcgga tgtacgtatg
     7861 aaaatcgttg aatatgacga cgtggaccca ttaaaggccc tgtgtgtaaa ccaattggcg
     7921 cttgaatttg cgctttcacc cgaaaaggtg gcgcatatcc gccaaacaga tccgcgccca
     7981 tttccttgcc tggcaattta tgctgttgaa gatggcgttg tcttgggtca gatagggatt
     8041 tttcgcctgc cgatgatatc aaccgaagga agggaagatg tggggggcgt ctgggccgtc
     8101 tcaacacatc cacaatacgc tgggcatgga gttgcctccc gactactgga agaggcgcat
     8161 actcgaatgc gcgatgctgg cttgcgcttt tccacactgg gtaccgaccg gtatcgcgtg
     8221 gcttataacc tgtatcgcca gcacgggtac gaggagacaa atgtttgggc taccgcgctt
     8281 gcgcgctggg aaacggccca ccagccgaca cgcctgcgcg cccagcttcc cgggcctgaa
     8341 ggccacgata ttgttgagca ggtatataag aatattgcca gtgactacct gggctttgcc
     8401 tggcggcaca ctccctttgt gcgcctgcat gacacagtta gccttgaaaa tatttggatc
     8461 ctttgggaac accaacagcc ggttgggtat gcgctggtcc aagttgacaa ggccatgttg
     8521 aagatcacca atttattgct gcgcgatggt atcaacgcag ctgaagcaat cgcggctgtg
     8581 gtggtgaaaa caaagacccc ttatatccag gtcagggtta gccgcccaag cgaggttgcc
     8641 agcctgcaac gcgcaggata tcaggttgcg cacccaacct gggacgcttt cctggtcaaa
     8701 tcgcttgtgc ctgaggtcac tgcggatgat gcccggcggc tgttcggcat tggcacagac
     8761 cgcttcttga tctcgtggct ggatgtgacg tgaaaaccag gacaaattca cctacggggt
     8821 ttggcgccac gtcgatcctc agaccgccaa acggcgcacg cgcgtggctg ctcggtccct
     8881 acgcagccga gggcaaaatc gccagcctat ctggtagacg acggagtatt aaatagaact
     8941 gaagacaata aaaggaaaac attggcgcag ggttaatgga aaaatatccg ccaatctcca
     9001 cgatgtttcg ctgaaattcg cgtggattgg cggaaattac ctgcggagag ggtgggtccc
     9061 ttctccacgg tgggtaaaaa ttcccgaact agcaccaggt tcattgctta agagacacgg
     9121 agatggtctc tccatctccg tgcctccgcg tcaacctatc agcgagtctt acaccgccct
     9181 gattaatcta aatccacctg cttccagaat tctctgaatt taaccactct catgacctgc
     9241 tccttcgtga ttaaaaatgg ggcaacatcg cccgccaggg catcaaaatc aatatccccg
     9301 atcttcagaa gcagttcctc gcgtagactt tggggagagg taatgcccat cttctgtttt
     9361 ataaatccaa aatccggcct ggttttactg aatagatatg tgatatcgta aaaatctctt
     9421 cctttcgggc gtttgcgatt aatcgcggta taaatctttt gtgataacaa aacatttaac
     9481 ggcgttactc tgatctcagt aaagacatca aatttattga gtatcttgac ctccggcaca
     9541 taggtgtacc cttgagctat cgtgtccagt tgaatgagta tcttctcctg atgaagaggt
     9601 gacaaacctt gcttgtaaag cagttctgga aaccgcagat cgcagtggaa tgcgcccttc
     9661 gcaaccatgc tggtctccac caggaatcct tccagcccta aaaacagctt caccttctgg
     9721 atcaattcac caaacgcctg cgagctcaaa ccaaaattgt caagatcgat atcttctgag
     9781 aaacggctat ttccatgtac gatgcgtaaa gctgtgccac ccagaaacga tagcatgctg
     9841 gcttgtttgc tttcaaaaat cgcctgtaag atcttatact gcaggtattc acgaaggatt
     9901 gcccgttcat acccctgcag cggttctgaa tattcatttt ttatttgctg taaatcaagc
     9961 attcagatac tccaggaatt gattgactct gtttttcagg gctcttttat cgaatagttt
    10021 gacatacttg gcgaaaatcg aatgatcaag cagcccacgt aattgtgtcc ggttccagcg
    10081 aagttcctca aaatcggcta aagactggat ctcagaattt aaatataaat agtccaataa
    10141 agccttttcc agataggcga tcttgaactt gatagcaggg gtattctcaa tcacctgata
    10201 accaatcata gtttgaggct tgacgctccg atagctgaat acaccccagg cgctttcata
    10261 ctgcttggtc ttgcgggagc tgatgcttgt cactcctaac acggtttcgg gaattatctc
    10321 gtaataagct aacgccgact ccaggctgac gtaggaaggc tcgtaaatct tgttcgccac
    10381 catgaataga accatctcat ccatcactcg atctgccaag atataatagc ctccagccag
    10441 tgatttgatg taaccctggt tatgccagta atttaattgc tgcctgtgaa aaccgggatc
    10501 gagcttgcgc acatcattca agttgaaggt tggcaaagct ctaatcctgg act
//
LOCUS       DBNHOH010000568         1826 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_161167, whole genome shotgun sequence.
ACCESSION   DBNHOH010000568 DBNHOH010000000
VERSION     DBNHOH010000568.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_161167"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="AC16V7_09890"
     CDS             <1..740
                     /locus_tag="AC16V7_09890"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014676846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6257470.1"
                     /translation="GYGLMIDKVPKATRQERVEEMLELVQLSGLTGRKPSQLSGGQRQ
                     RVALARALIKHPRVLLLDEPLGALDLKLRQQMQIELKAIQQRVGITFVYVTHDQEEAL
                     TMSDRIAVFNRGKSEQVGTPAEIYEHPATPFVAGFVGVSNLVSGEAAQKITGSADTFS
                     IRPEKIRLAEPDLDVPANMYSADGTVRDVLYLGLYTRYLIDLELGEDLVVIQQNLDAT
                     SMDVLAARGKKVRLLWLRDHNRALKSA"
     gene            784..1725
                     /locus_tag="AC16V7_09895"
     CDS             784..1725
                     /locus_tag="AC16V7_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6257471.1"
                     /translation="MSVSPGKPGRSSLSTRVSSYLYKRPTAILLLLLGPPMAWMFIIY
                     LGSLFALLVNSFFSLDGYTGLVVRQFTLNTYRDLFTRANFDIVQRTAGMAAAVTVTAA
                     ILAFPLALFIARYASRRVKGILYLAVLLPLWSSYLVRVYAWKLILAKEGIVSWFFNIL
                     HLDGVLNSLLSLPVIGGPSLSVSNIGIFIVFVYVWLPYMILPIQAALERVPKSLLEAS
                     GDLGARPMQTFRLVILPLAFPGVVAGSIFSFSLTMGDFIVPTALGNSSYFIGQAVLAH
                     QGTSGNIPLAAAFTMIPIVIMIIYLMVARRLGAFEAL"
BASE COUNT          352 a          493 c          514 g          467 t
ORIGIN      
        1 taggttacgg gctgatgatc gataaagtgc ccaaagccac acggcaagag cgtgtggagg
       61 agatgctgga actggtccag ctatccggtt tgaccggtcg caagccgtcc cagctctctg
      121 gtggacagcg ccagcgcgtc gccctggcgc gagcgctgat caagcaccct cgcgtgctgc
      181 tgctcgatga gccacttggc gccctggatc tgaaactgcg ccagcagatg cagatcgaat
      241 taaaagccat ccagcaacga gtggggatca cttttgtcta tgtcacccat gatcaagaag
      301 aggcgctgac catgagcgac cgcatcgcgg tgttcaaccg cggcaaaagc gaacaagttg
      361 gcaccccggc ggagatttac gagcatccag cgactccctt cgtggctggg ttcgtgggtg
      421 tctccaacct ggtgagtggc gaagcagcgc agaagatcac tggttctgcg gatacctttt
      481 ccatcagacc ggagaagatt cgcctggcag agccagattt ggatgtgcct gcgaacatgt
      541 attcagccga tggcactgtg cgggatgtcc tttacctggg tctttacacc cgctacctga
      601 tcgatctgga gttaggtgag gatctcgtgg tgatccagca gaatctagac gcaacttcga
      661 tggatgtgct ggcggcgcgc ggcaagaaag tgcgtctgct ctggttgcgc gatcataacc
      721 gggctttgaa gagcgcgtag aacgatggct actctctccc catcaccgtc aggtgacctt
      781 caaatgagcg tttctccggg taagcctggg cgctcttccc tgagcacccg agtctcgtcc
      841 tacctgtata agcgccccac ggcgatactg cttctacttc tcgggccgcc tatggcctgg
      901 atgtttatta tctacctcgg ttcgctgttc gccctgctcg tcaatagctt tttcagcctg
      961 gatggctata ccgggctggt tgtgcgccag ttcaccctga atacataccg cgacctgttt
     1021 acgcgcgcta attttgacat tgtgcaacgc acagccggga tggctgctgc ggtgaccgtc
     1081 accgcagcga ttctggcttt ccctctggcg ctgtttatcg cccgctatgc atcacggcgg
     1141 gttaaaggga tcctgtatct ggcggtattg ctgccactct ggtcgagcta cctggtgcgg
     1201 gtatatgcct ggaagcttat cctggccaaa gagggtattg tgagctggtt cttcaatatt
     1261 ttgcatctgg atggcgtgtt gaactcgttg ctcagcctac cggtgatcgg ggggccatcc
     1321 ctctcggttt ccaacatcgg gatattcatc gtctttgttt atgtctggct gccttatatg
     1381 attctcccca tccaggctgc cctagagcgg gtgccgaaat ccttacttga ggcatctggc
     1441 gacctgggag cgcggccaat gcaaaccttt cgccttgtga tcctgcctct ggcattcccg
     1501 ggtgttgtgg cgggctcgat tttttcattc tcgctcacca tgggtgattt tatcgtgccc
     1561 accgcgttag gtaattccag ctatttcatc ggccaggcgg tgcttgccca ccagggtact
     1621 tcgggcaata ttcccctggc agctgccttt acgatgattc cgattgtcat catgattatt
     1681 tatctcatgg tagccaggcg tttaggagct ttcgaggcgc tctaataaaa tgcgtaaatc
     1741 ctgggaatgg tgcacgatga taggtttcga tagttttctg gagagacgtg cgtatgcaaa
     1801 aggaagatag atctggcgat aaatcc
//
LOCUS       DBNHOH010000569         2355 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_674729, whole genome shotgun sequence.
ACCESSION   DBNHOH010000569 DBNHOH010000000
VERSION     DBNHOH010000569.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_674729"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="AC16V7_09900"
     CDS             <1..914
                     /locus_tag="AC16V7_09900"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017717200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HIR6257472.1"
                     /translation="AQLMFDRLGDRVSMWSTHNEPWVLAFLGHGTGEHAPGICNYAMA
                     YQVAHHLLLGHGKAVQVFRQSARQGQIGIILNLNYFLPASDREADIAACRRVYMENVS
                     LFLEPLFFGRYPQELFDWIGFHQPKIHDADLDIIRQPIDFLGVNHYFSESVSHAVNGP
                     VLKARNEPYSALGWGRTTMNWGINPQGFEAVLLDIKDNYGNPTVFITENGCSFPDVAD
                     EHGVVADWDRVNYLRAHMHAAYRALQAGLDLRGYFVWSLMDNFEWAWGYSRRFGLVRV
                     DYPTQKRIPKQSATWYSQVIAANGIDI"
     gene            947..>2355
                     /gene="treS"
                     /locus_tag="AC16V7_09905"
     CDS             947..>2355
                     /gene="treS"
                     /locus_tag="AC16V7_09905"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HIR6257473.1"
                     /translation="MTGNSLDQDTLWYKNAVFYEVYIRAFYDSNGDGHGDLRGLKEKL
                     DYLQDLGVDCLWLMPIYPSPLKDDGYDIADYYCILETYGTLQDFKDLLDAAHERGMRV
                     ITDLVLNHTSDQHAWFQASRSSRESPYRDYYVWSDTDQKYQEARIIFVDTQKSNWTWD
                     PGSGQYYWHRFYPSQPDLNFDNPAVQQEMLNAVKFWLDMGVDGFRADAVPYLFERDGT
                     NCENLPETHAFLKRLRRFMDENYPGRILLCEANQWPEDVRPYFGDNDEFHMGFHFPLM
                     PRIYMALRKADKQPLEWVLKRTPPIPEDCQWCVFLRNHDELTLEMVTEEERQWMWQEY
                     APDARMRLNLGIRRRLTPLLDNDRRKIDVANSLLFTMPGSPIIYYGDEIGMGDNIWLP
                     DRNGVRTPMQWEDGLNAGFSSATEDQVYAPVIQDDPYGVQWVNVAAQQADPSSLWHAI
                     RKMIKVRKANPKLGWGDFI"
BASE COUNT          572 a          512 c          631 g          640 t
ORIGIN      
        1 atgcccaatt gatgtttgac cggttggggg accgggtaag catgtggtct acacacaatg
       61 agccttgggt tttagctttt ctaggtcatg gaactgggga gcacgcacct gggatttgca
      121 attatgccat ggcctaccag gtagctcatc atctcttgtt agggcatggg aaagctgttc
      181 aggtcttccg ccagagtgct cgtcaagggc aaatcgggat tattctcaat ctcaattatt
      241 ttttgccagc cagcgatcgc gaagctgata tcgccgcatg ccgccgtgtc tatatggaga
      301 atgtgagtct atttctggaa ccattattct tcggtcgcta tccccaggag ctgtttgact
      361 ggataggctt ccaccagcca aaaattcatg atgctgacct ggatattatc cgccaaccga
      421 ttgatttctt gggtgtcaac cactatttca gtgaatctgt ctcgcacgcg gtaaatgggc
      481 cggtgctcaa ggcaagaaat gagccgtact ctgcactcgg ttggggacgc accaccatga
      541 attggggcat taacccacag gggtttgaag ctgtattgtt ggatattaag gacaattatg
      601 gtaatccaac tgtctttatc acggagaatg gctgttcgtt tccagatgtt gcggatgaac
      661 atggtgttgt cgctgattgg gatcgggtga attatttgcg tgcgcatatg catgcagcgt
      721 atcgggcatt gcaggcaggg ctagatttgc ggggctactt tgtctggagt ttgatggata
      781 acttcgaatg ggcttggggt tacagtcggc ggtttgggtt ggtccgtgtt gattacccaa
      841 cacagaagcg aatccctaaa cagagtgcta cgtggtacag tcaggtcatt gcggcaaatg
      901 ggatcgatat ataaatcatg atgattgtga aaaggaaaca atttaattga caggaaattc
      961 attagatcag gatacattgt ggtacaaaaa cgctgtcttt tacgaagttt atatccgcgc
     1021 tttttatgac agcaacggtg atggacacgg tgatctgcgc ggtctgaaag aaaaactgga
     1081 ttacttgcaa gatctgggcg tggattgcct gtggttgatg cccatctatc cttcacccct
     1141 gaaggatgat ggctatgata tcgccgatta ttattgcatt cttgaaacct acggcacgct
     1201 ccaagatttc aaggatctac tggatgctgc gcacgagagg ggcatgcgcg tgatcacaga
     1261 tttggtgctt aaccatactt cagaccagca tgcctggttt caagcctctc gatccagccg
     1321 agaatcacct taccgggatt attatgtgtg gagcgacacg gatcagaagt atcaagaagc
     1381 gcgcatcatc ttcgttgata cccagaagtc gaactggacc tgggatccag gctcgggcca
     1441 gtattattgg caccgctttt acccctccca gccagattta aactttgata atcctgctgt
     1501 ccaacaggaa atgctcaatg cagtcaagtt ttggctggat atgggtgtgg atggttttag
     1561 ggcagatgct gttccctatt tatttgagcg agatggcacc aattgtgaga acttgcccga
     1621 gacgcatgca ttccttaagc gcctgcgccg ctttatggat gagaattatc cgggcagaat
     1681 attgctctgc gaggccaacc aatggcctga ggatgtacgg ccctactttg gcgacaacga
     1741 tgaattccat atgggattcc attttccgct tatgccccgt atttacatgg cattgcgtaa
     1801 agccgataaa caacctctgg agtgggttct gaagcgcaca ccacccattc ccgaggattg
     1861 ccagtggtgt gtatttttgc gcaatcacga cgagttaact ttggaaatgg tcacagaaga
     1921 agagcgccaa tggatgtggc aggaatatgc tccggatgcg cgcatgcgct tgaatttagg
     1981 tatccgccgt cgtctgacac ctttattgga taatgaccgg cgtaagattg atgtagctaa
     2041 ctcgctttta tttaccatgc caggctctcc gatcatttat tacggggatg agatcggtat
     2101 gggagataat atctggctac cagatcgcaa tggtgtgcgc acaccgatgc agtgggaaga
     2161 tgggctgaac gctggattct cgtctgcaac agaagaccag gtttacgcac cggtgatcca
     2221 ggatgatcct tatggtgtgc aatgggtaaa tgtcgctgca caacaagccg atcctagctc
     2281 gttatggcac gcaatacgca agatgattaa ggtgcgtaag gcgaatccga aattgggttg
     2341 gggggatttt atctt
//
LOCUS       DBNHOH010000570         2966 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_33676, whole genome shotgun sequence.
ACCESSION   DBNHOH010000570 DBNHOH010000000
VERSION     DBNHOH010000570.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_33676"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="AC16V7_09910"
     CDS             <1..299
                     /locus_tag="AC16V7_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR6257474.1"
                     /translation="SGPPVVIYGNSRRWLPVEFKSNLQGFFFFNSIVVVFGHALSQNI
                     TPLVWQDYLMAIPALALGILAGVSLDRMINPVVFRRLVLGLLLVMGGRLILQGF"
     gene            434..808
                     /locus_tag="AC16V7_09915"
     CDS             434..808
                     /locus_tag="AC16V7_09915"
                     /inference="COORDINATES: protein motif:HMM:NF024645.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR6257475.1"
                     /translation="MKSTAAWVVAIVLILVVLLALPGVLRFAGLSGPWYGGMMGGRGM
                     MGGYYGFFPLGFLGMGFMLLVPVALLVLVVAGIVAVVNSLNRGNSPASYPTAGNSRAC
                     PNCAKPAQPDWNTCPYCGTSLN"
     gene            complement(815..964)
                     /locus_tag="AC16V7_09920"
     CDS             complement(815..964)
                     /locus_tag="AC16V7_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257476.1"
                     /translation="MLKLLVEYAETAARYELECLRLGSPTQGLTDEQLLQLCTQFGID
                     PNRFV"
     gene            1332..2117
                     /locus_tag="AC16V7_09925"
     CDS             1332..2117
                     /locus_tag="AC16V7_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012669.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR6257477.1"
                     /translation="MTTYERRHSLLEILRKHPGLRVSEMAKALGVSEGTVRNDLNALK
                     EEGRLKRVHGGAVLIDQDQFQNDSFVRRYNQNVAAKLAIAREAALLVMDGDSILLDAS
                     STSYYLAHAISARNRLRVMTNGFEVARELAQNTSNTVILIGGVVNNDSSSVTGLLSEQ
                     IIAEMHIEKAFFSCSGFSVERGMTEVHFEEAQLKRKAIESTKQVIALIDSSKFGKEDL
                     TPFARPMQITHLFTDAGITSEWKTRLQETNILFTVCGEDSIAI"
     gene            2163..>2966
                     /locus_tag="AC16V7_09930"
     CDS             2163..>2966
                     /locus_tag="AC16V7_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6257478.1"
                     /translation="MMTSVEAKAPILQIRHISKRFDTTQALDDVSLDLYPGEIHALMG
                     ENGAGKSTLIKIMTGIYHSDEGEMLLDGKPIRVSNSIDAQAYGIAAIYQEPMVYPDLN
                     VAENIFISHRSQGVIVNWRKMYQDAEDILAKLDVKLDVRMPARGLTLAAQQTIEIAKA
                     ISLNVRLLIMDEPTASLSAHEVEQLFKLTRSLRNQGVSILFISHRMEEVFEIADRVTI
                     FRDGRWISTLPRSEITRERVIRDMVGRKIEDFFAKSGVQRGELLMSVQGL"
BASE COUNT          707 a          656 c          801 g          802 t
ORIGIN      
        1 cgtctggccc gccggtggtc atatatggta actcccggcg ctggctgccg gtggagttta
       61 agagcaacct tcagggattc ttctttttta acagcatcgt ggtggtcttt gggcatgcct
      121 tgagccagaa tatcaccccg ctggtttggc aggactactt gatggcgatc cccgccctgg
      181 cgctgggtat cttggctggg gtcagcctgg ataggatgat caatccagtc gttttccgca
      241 ggctggtgct gggtttgctg cttgtgatgg gtgggcgctt gattttacag ggtttttgag
      301 atgaaccagg tttgtgttga tctctttgcc aggtattcac gaattcttca caactttttc
      361 acattgagtt ggtatcctga agatgtgctg aaatctaaag gttgtttttc tcaatgcttg
      421 aaggaggttt gacgtgaaat ctacagctgc atgggtggtt gccattgtat tgatcttggt
      481 cgttttgcta gccttacccg gcgtgctgcg cttcgctggc ctgtctggac catggtacgg
      541 cggtatgatg ggtgggcgcg gcatgatggg aggttattac ggctttttcc ccttgggttt
      601 tttggggatg ggttttatgc tgctggtgcc ggtggctttg ctggtgttgg tggtggcggg
      661 tattgtagcc gtggtgaata gcctgaaccg cggcaactct ccggcctcgt atccaactgc
      721 tgggaatagc cgcgcttgcc cgaattgtgc caagcccgcc cagcctgact ggaacacctg
      781 cccctattgc gggacatcct tgaattagtc tttttcagac aaaacggttc ggatcaatgc
      841 caaattgcgt gcaaagctgg agcaactgtt cgtctgtgag accctgagtg ggtgagccca
      901 ggcgcaggca ctccagctcg tagcgcgcgg ctgtctcggc gtattccacc agtaatttaa
      961 gcaaacgcat ttccgatgtc ggaaatgcag cagaagccgg taacctggac tttataccta
     1021 agctgaggtt attttgtgat atccaataaa tgatcataag ggacagtcgt taaagggatt
     1081 tattttatac atctggctgt cagggtggat gtcgctgtgg taattggtgt cgttggtcag
     1141 tcgttcaatt gtggcacccc ccatgttcat tttagggtcg cgccggtctt cctgttctct
     1201 ttgatttccc ccaccgattt tgggacagac actgaaatat tatttaaata ttgacaaata
     1261 atgacaaaaa ttgtatgata gttttatcga aatcaacaaa aaacaacaaa atattctaag
     1321 gattgtctga tttgaccact tacgagcgcc gacactctct attagaaatt ctgcgtaaac
     1381 atcccggttt acgtgtctcg gagatggcta aggcgttagg tgtatccgaa ggtaccgttc
     1441 gaaacgacct caatgctcta aaagaggagg gacgcctaaa gcgcgtacac ggaggagctg
     1501 tcttaattga ccaagaccaa tttcaaaatg attcttttgt acgtcgttac aatcaaaatg
     1561 tagctgccaa gctcgctatt gcgcgtgagg cagctctttt agtcatggat ggtgactcga
     1621 ttctgctgga tgcgagcagt acatcctatt acttggcgca cgcaatatcc gcaagaaaca
     1681 gattgcgcgt gatgaccaat ggatttgaag ttgcgcggga gttggctcaa aacacctcga
     1741 acaccgtcat tctaattggg ggggtagtca ataacgactc ctcttccgta acgggtctgt
     1801 tgagcgagca gatcattgca gaaatgcaca tcgaaaaagc gtttttttct tgcagtggtt
     1861 ttagcgtcga gcgcgggatg acggaagtgc actttgagga ggcgcaactg aaacgtaaag
     1921 caatcgaatc tacaaaacag gttatcgcgt tgattgactc ttcgaaattc ggcaaagaag
     1981 acctgacccc tttcgctcgt ccgatgcaaa ttacgcactt gtttacagat gccggaatta
     2041 ccagcgaatg gaaaacccgc ctgcaagaaa ctaacatctt atttacagtc tgcggagaag
     2101 attcgattgc gatctgagcg gtgagaggcc attgagccac caattgaaaa aggcattgat
     2161 caatgatgac gagtgttgaa gcaaaagctc ctattctgca gataaggcat atttccaagc
     2221 gcttcgatac aacccaggca ctggatgatg tgtctcttga cctttatccg ggtgagattc
     2281 acgccttgat gggggagaac ggcgctggta aatcaacgct gattaagatc atgacgggga
     2341 tatatcattc ggatgaaggg gaaatgttgt tagatgggaa acctatccgg gtgagtaact
     2401 cgatagatgc ccaggcctac ggcattgctg caatctacca ggagccgatg gtatatccgg
     2461 atttgaacgt cgccgagaat atttttattt cgcaccgttc tcagggggtg atcgtaaact
     2521 ggcgtaagat gtaccaggat gcggaagata ttcttgcaaa gttagatgtt aagctagacg
     2581 tgcgcatgcc agcgcgcgga ttgacacttg ccgcccagca gactatcgaa attgcgaaag
     2641 ccatctcact caacgtgcgc ctcctgatca tggatgaacc gaccgcctcc ctctcagccc
     2701 atgaggtgga acagttattt aaactgacgc gctcgctgcg caatcagggc gtgtcaattc
     2761 tctttatcag tcaccgtatg gaggaagtat tcgaaattgc ggatcgcgtg accatctttc
     2821 gtgatggcag gtggatttca acgctgccac gcagcgagat aacacgcgaa agagtcatcc
     2881 gagatatggt tgggcgcaag atcgaagatt tttttgcaaa aagcggggtg cagcgcggtg
     2941 agctgttgat gtcagtacaa ggtctg
//
LOCUS       DBNHOH010000571         1852 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1251568, whole genome shotgun sequence.
ACCESSION   DBNHOH010000571 DBNHOH010000000
VERSION     DBNHOH010000571.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1251568"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..1372
                     /locus_tag="AC16V7_09935"
     CDS             122..1372
                     /locus_tag="AC16V7_09935"
                     /inference="COORDINATES: protein motif:HMM:NF018485.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015112 - nitrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6257479.1"
                     /translation="MNKLQASIYPLHYAWIIVAISTLVVFAALGLARFGYSSLLPPMQ
                     ASLGMNNTQAGIMATFNLIGYLTLSVIGGALAARYGPRVVITVGLALVGVAMLFTGMA
                     NTFLSAVLWRTVTGVGSGASNVPVMALVAAWFAARRRGLGSGIAVSGSSIGLIMIGSL
                     VPQVLSAYGEDGWRISWFIFGITTLILALLGWLILRNDPKEVDQSPYGLEDPQPGPKA
                     TGTGLQWGRVYRAPAVWHLGLVYIAFGFSYIIYLTFFIKRLIAEVGYTQEAAGGLFMT
                     MGWFSMLCGLIWGTISDKIGRKNALILVYLIHATSFALFAWNGSINLTLSAILFGLTA
                     WSIPAIMAATCGDVLGPRLAPAALGFITLFFGIGQALGPSVAGAMADSASSLIPAYLL
                     ASGVALLGAFGTLFLRPPSTARNN"
     gene            1382..>1852
                     /locus_tag="AC16V7_09940"
     CDS             1382..>1852
                     /locus_tag="AC16V7_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014345529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR6257480.1"
                     /translation="MNDIQASLRGSIVPLVTPFKNGELDEAALSGLIEWQIASGSHGL
                     SVTGTTGEPSALSTRERERVIQVAVEAISGRLPLLAGTGSNNLEETLRLSLFAQKTGA
                     DAILVVVPYYIRPSQEGLFGYFKTVADSVELPVILYNIPGRSAINLEIDTVSRLR"
BASE COUNT          349 a          529 c          542 g          432 t
ORIGIN      
        1 ttcaggccat gctggacgac tactacacag agcgcggctg ggataaccag gggcgggttc
       61 ccgtagatag gcaacaagca atcgacgaaa tgattgctca attggagtga taagtaggcg
      121 catgaacaag ctgcaagcct caatctatcc cttgcattac gcctggatca tcgtggcgat
      181 cagcaccctg gtggtcttcg ccgcccttgg actggcgcgc tttggctatt cctccttatt
      241 gccgcccatg caagcctccc tgggaatgaa caacacccag gctggcatca tggcgacctt
      301 taacctcatt ggatacctga cgctatctgt gatcggcggc gcgctggccg cgcgctacgg
      361 tccgagggtg gttatcactg tagggcttgc cctggtaggc gtggcaatgc tcttcactgg
      421 gatggctaac acatttctct cggctgtgct gtggaggaca gtcacggggg tgggcagcgg
      481 ggcaagcaac gtgccggtca tggcgctggt cgcggcctgg tttgccgccc ggcgcagggg
      541 tctaggttcc ggcatcgcag tctccggttc gtccatcggc ctgatcatga ttggctcgct
      601 tgtcccacag gttctctcag catacggaga ggatggctgg cgaataagct ggttcatctt
      661 tggtatcacc acgctgatcc tggctcttct tgggtggcta atcctgcgaa atgacccgaa
      721 ggaagttgac cagtcccctt atgggcttga agatccgcaa ccaggtccaa aagcaacagg
      781 cactgggctg caatgggggc gcgtttaccg agctccagcg gtctggcatt taggcctggt
      841 ttacatcgca ttcggctttt cctacattat ctatttgacc ttttttatca agcgcctgat
      901 cgccgaggtc ggctataccc aggaggccgc cggaggcctg ttcatgacca tgggctggtt
      961 cagcatgctg tgtggtctca tctggggaac catctcggac aagatcgggc gtaaaaatgc
     1021 attgatcctg gtttacctga tccatgcgac ttcgttcgcg ttatttgcct ggaatggctc
     1081 tatcaacctc acactttcag cgatcctgtt tggcctgacc gcctggagca ttcccgcaat
     1141 tatggcagcc acttgcggtg atgtcctggg accgcggttg gccccggcag ccctgggttt
     1201 catcaccctg tttttcggta tcggtcaggc gctgggaccc agtgtagccg gcgcgatggc
     1261 cgattccgcg tcctcgctca tcccagccta tctcttggca agcggagtgg cgctgctggg
     1321 tgcctttggc acactctttc tgcgcccgcc ttcgaccgcg aggaataatt gaaggcacag
     1381 catgaatgat attcaggcat ccctgcgagg ctcgattgtc cccctggtga cccccttcaa
     1441 gaatggggag ctggatgaag ctgccttatc cggattgatc gaatggcaga tcgccagcgg
     1501 cagccacggt ctctcggtga caggcacgac tggcgaaccc agcgccttga gcaccaggga
     1561 acgcgagcgt gtgatccagg tagcggtcga agccatatct gggcgcttgc ccctcctggc
     1621 aggtaccggc tcaaacaacc tggaagagac cttgcgcctc tcgctctttg cgcaaaaaac
     1681 gggggcagat gccatcctgg tggtagtgcc gtactacatc cgccccagcc aggaagggct
     1741 gtttggctac ttcaagacgg tcgctgattc tgtggaactg ccggttattc tgtacaatat
     1801 cccaggcagg tcagcgatta acttggagat cgacactgtc agccggctgc ga
//
LOCUS       DBNHOH010000572         2746 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1508033, whole genome shotgun sequence.
ACCESSION   DBNHOH010000572 DBNHOH010000000
VERSION     DBNHOH010000572.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1508033"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="AC16V7_09945"
     CDS             complement(<1..662)
                     /locus_tag="AC16V7_09945"
                     /EC_number="3.5.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF039614.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016403 - dimethylargininase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylarginine dimethylaminohydrolase family
                     protein"
                     /protein_id="HIR6257481.1"
                     /translation="MFTHAIVRKPGMNFAQGITSANLGMPEYARMVDQHQAYIQALES
                     LGLQVIVLDAEPNYPDAYFVEDVAVVTPDVAVITNPGAPARKGEEDTLESVLARFRPI
                     VRIQPPGTVEGGDVLMVGTHFFIGLSERTNLEGATQLGNVLERYGNTFTLVHIQAGLH
                     LKSSVNYAGKNTLLVCEDFAERGEFQGYHTIVVPQIEAYAANTLWANDHLITPAGFPE
                     TR"
     gene            748..1317
                     /locus_tag="AC16V7_09950"
     CDS             748..1317
                     /locus_tag="AC16V7_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257482.1"
                     /translation="MSDQVSIESERKPLLSARQVGVAAAFGGAALALVLAGVTIPIPG
                     TPVVTDPREIFTTTGASLTGPIGGIVIGILAGIAEPGIPLASLLAHIIGGIYSGFLYK
                     NLAWRFRENRVSYLALWALQVLGYYFIVVVPLFVVGLVLFYPDPEYGGFFALLAALEG
                     GAAPEAIITTVVTTIIMAALPERYRRPLW"
     gene            1492..>2746
                     /locus_tag="AC16V7_09955"
     CDS             1492..>2746
                     /locus_tag="AC16V7_09955"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6257483.1"
                     /translation="MDYDITFRNVSFTYEGADIPALQDITLTIMPGEIVLITGPAGSG
                     KTTLCSCMNGLIPHYHEGELTGEVLVRTYNTRKARIGGLASLVGMVFQDPESQLVTNS
                     VADEVAFGPENLGVAREDINLRVQEALQATRLKGYEDREPHSLSGGEQQACVIASIYA
                     MHPEIYVMDEPLANLDPVGRVQVLRVVVEIAKQRGKTLVLVEHSLEEVLPLVDRVIVL
                     DQGRIIRDSSTADVLAAGDIPHVFTRPPIIRLAEKFSLTPLPLTSDQFYQSFSEKYPL
                     RSTNWTVPEKPVLKAGTPVIEMRDVNYSYDNKNIVLNDVNLTINAGELVAILGRNGSG
                     KTTLVRHIIGLLQPIRGKVIVMGKDVSVTPTHEIAQDVGFCFQNPNHQIVSFNVRDEL
                     LFGLKAHHISPTEFETRIHEALEFVD"
BASE COUNT          664 a          717 c          681 g          684 t
ORIGIN      
        1 ttcctggttt cgggaaaacc cgctggcgtg atcagatgat cgttggccca cagagtgttg
       61 gcagcatagg cctctatctg gggaacaaca attgtatgat acccctgaaa ttcccccctc
      121 tcggcaaaat cctcgcatac cagcaaggtg tttttgcccg cataattcac actggatttc
      181 agatgcagtc ctgcctggat atgtaccagt gtgaaagtat tgccatagcg ctctaggaca
      241 ttccccagtt gtgttgctcc ctctaaattc gtgcgttccg agaggccgat gaaaaagtgc
      301 gttccaacca tgagcacatc cccgccttcc accgtgccag gcggctggat gcgcactatc
      361 gggcggaagc gcgctaatac ggattccaga gtatcttctt cgcccttccg cgctggcgcc
      421 cccggattgg tgatgaccgc cacatccggt gtgacaacag ccacatcttc aacaaagtag
      481 gcatccgggt agtttggttc ggcatccagc acgatcacct gcaaccccaa ggactcaagc
      541 gcctgaatat atgcctggtg ttgatcgacc atgcgcgcat attctggcat tcctaaatta
      601 gcgctggtaa tgccctgggc gaaattcatc ccgggtttac gaacgatggc gtgggtgaac
      661 atttgggaac ctcctggtgt tatcgtaact ttcaacatgc tagagggttt aagttcaaaa
      721 taacccgttt tcagagagga gatttcaatg tctgatcagg taagtattga aagcgaaaga
      781 aaacctttgc tttccgcccg ccaagtgggc gttgcagctg catttggtgg tgcagctttg
      841 gctcttgtcc ttgccggtgt aaccatcccc atcccgggca caccggtagt taccgacccg
      901 cgtgagatat ttaccaccac aggcgcctcg ttaacaggtc cgatcggtgg tattgtgatt
      961 ggtattctgg cgggcatcgc tgaacccggt atccctctcg ccagcttgct ggcacatatc
     1021 attgggggga tttacagtgg cttcttgtat aagaacctcg cctggcgttt ccgtgaaaat
     1081 cgggtatctt acctggcatt atgggctctt caggtattag gctactactt catcgtcgtc
     1141 gtacccttgt ttgtagtagg attagtctta ttctaccctg atcctgaata cggtggtttt
     1201 tttgccttgc tcgccgcgct ggaaggcgga gccgctccgg aagctatcat aacgacagtc
     1261 gtaactacca tcattatggc ggctttgcca gagcgttatc gacgtcctct ctggtaatct
     1321 taatcaaagc ccaacaattt tgggtgataa tataacccct aagccgcatc ctggaaacag
     1381 gttgcgactt gggctattaa ctttttagga ctgacgcggt tggaggaaat ggggggcagc
     1441 cccccatttc ctcgccaacc gtaacgactg aatcttcaag aaaacaggtt tatggattac
     1501 gacataacgt tccgcaatgt gtcattcaca tacgagggag ctgatattcc agcactgcag
     1561 gatattaccc tcacgatcat gccaggtgaa atcgttttaa tcactggccc tgccggcagc
     1621 ggcaagacga cactgtgtag ctgcatgaat gggttaatcc cgcactatca cgagggtgaa
     1681 ttaacaggtg aggtgcttgt gcgcacctac aacacccgca aagcccgcat cggcggatta
     1741 gcctcactgg tcggaatggt ttttcaggat ccagaaagcc agcttgtcac caatagtgtc
     1801 gccgacgagg tcgcctttgg accggaaaac ctgggcgtcg ccagagaaga catcaatctg
     1861 cgtgttcaag aggcgcttca ggcaactagg ctcaagggtt atgaagatcg tgagccgcat
     1921 agtctttcag ggggggaaca gcaggcatgc gtaatcgcct ccatctacgc catgcaccca
     1981 gaaatttacg tcatggatga gcctctcgca aacctagacc ccgttggcag ggttcaggtt
     2041 ctgcgtgtgg tcgttgaaat agccaaacag cgaggaaaga cattggtgct ggtcgaacat
     2101 tcgctggaag aggttctgcc tttggtcgat cgtgtgatcg tacttgacca agggcggata
     2161 atccgcgata gctcaacagc tgatgtactg gctgcgggcg atatacccca tgtctttacc
     2221 cggcctccaa ttatccggct cgcagaaaaa tttagcttaa cccccttgcc actcacctcc
     2281 gaccagtttt atcaatcctt cagcgaaaaa tatccattga gatcaaccaa ctggaccgta
     2341 ccagaaaagc cagttttaaa ggctgggaca ccagtcattg aaatgcgaga cgtaaattat
     2401 tcctacgata ataaaaatat cgtcttaaat gatgtcaacc tcaccatcaa cgcgggagaa
     2461 ttagttgcaa tcttagggag aaatgggtcg ggtaaaacca cactggtgcg ccacatcatt
     2521 ggcttgttgc aacccatccg tggcaaagtg atcgtgatgg gcaaagacgt atctgtaaca
     2581 ccaacccacg agattgccca ggatgtaggc ttctgcttcc aaaaccccaa tcaccagatc
     2641 gtctctttca atgtgcggga tgaattgcta tttggcctta aagcgcatca tatctccccc
     2701 accgaatttg agacgcgcat ccacgaagcc ttggaatttg tcgata
//
LOCUS       DBNHOH010000573         4313 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_33807, whole genome shotgun sequence.
ACCESSION   DBNHOH010000573 DBNHOH010000000
VERSION     DBNHOH010000573.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_33807"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="AC16V7_09960"
     CDS             complement(<1..1022)
                     /locus_tag="AC16V7_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6257484.1"
                     /translation="MNRKAISTILSVFFILSFVLAACAPKPTPTEPPAAVEPEAPEMT
                     EAPPAMPEVDFAMMPGGFLERALTGEFSGTTVTVDGAFEGNDPDGVKMDRSVAQFEEL
                     TGINVNYIGNKEFEATISVRVEAGDAPDIVDFPQPGKMANFVRDGKVVDVTSWMTEEW
                     LSQQYNQSWRDMATIEGVEAGVFHRFNGKSLVWYPKAAWDAAGYEIPETWDDLMALNQ
                     MIADDGDTAWCIGIESGAATGWTATDWTEEMMLRTTSLENYDAWVAGTLPFSSPEVKK
                     AIETWSELWFNDDYVFGGRASIVSTFFGDSPAPMFEDPPKCWMHKQGNFITGFFPEGV
                     EAGVDYG"
     gene            complement(1138..2193)
                     /locus_tag="AC16V7_09965"
     CDS             complement(1138..2193)
                     /locus_tag="AC16V7_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR6257485.1"
                     /translation="MTNHRITSQDVAEKAGVSRTTVSLVLNNVPGVKISPQTRRRVLD
                     AARELNYVPEAAAQALASRRARIIGLILIRNPHQISTDAFLTQILDGLIETIRPSGMR
                     LLIDIVEPQHQKETYLQLIQAKHIDGIILSGPRYDDDALSSLLEVNFPAVLLGQIPDS
                     DFAFVDVDNRAAVNTAVAHLINLGHSRIACITNAPISYTAALDRLTGYKQALKENGIA
                     LDSELIRYGDFDIESGYRQMHSLLASNVTMTAVFIGSDTLAYGAVAAIRERGLRIPQD
                     IAVVGFDDIPFSRYSDPPLTTVRLPAGDLARRSAEILSQLLAGDEPESKQVILETQLV
                     VRDSCGASRSNRRMKLQ"
     gene            complement(2313..3110)
                     /locus_tag="AC16V7_09970"
     CDS             complement(2313..3110)
                     /locus_tag="AC16V7_09970"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6257486.1"
                     /translation="MTIALRTDEITKIYGKDTSAVVAVDQVSLEVKRGEFIALVGPSG
                     SGKTSMLAMLAALLSPTSGSIFIGDVALAEMKEKERTRFRREKIGFTFQTNNLVPYLT
                     ALENVELMLRLNKTLNKEGKAHAKELLARLGLQERLNSLPNQLSGGQQQRIAIARALV
                     HRPEVVLADEPTANLDTERAFQVVETFANLIHEQNRAGIMVTHDLRMTKYVDRVIQMV
                     DGKVTRSLDDRAAIDLLAQTSSFEAAEQYVGSTPFQYAVAPAASKVA"
     gene            complement(3161..>4313)
                     /locus_tag="AC16V7_09975"
     CDS             complement(3161..>4313)
                     /locus_tag="AC16V7_09975"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6257487.1"
                     /translation="MSFYLALKEIWRLRGRYLLFSMVIALITILVLFIAALAGGLANA
                     NKQLIEKLDGEMLIYQNNVDLQLTASRLGQSKLSDIRRIDGVADVGPVGLATATVVFA
                     DGRSPIDIALVGVEPGKPGDPPLMAGPGLRTKRNNDAVIDEDLASRAGLTIGDRFIIK
                     SVQGTEEEFYELSVVGITDSRQYFFLPSAIMPIRTWSKVRPQGDGESSTSEIIFNLAA
                     VKLQDPQQFDLVAQKLEYFVDGIEVADKQAAILALPGYTAQQSTLNTQQIFTLLIGVL
                     VVGGFFQIQTLQKVAQIGVLKAIGSSSATVAFATLLQIILVTLIGVGIGSLGTMLLVL
                     GMPSEIPILFTGSTVISAAVLLLFIGPIGGLVSIRLALKIDPLTAIGLS"
BASE COUNT         1040 a         1170 c         1048 g         1055 t
ORIGIN      
        1 ccatagtcta cgcctgcttc cacaccttcg gggaagaagc cggtgatgaa gttgccctgc
       61 ttgtgcatcc agcacttggg cgggtcttcg aacatcggcg ccggggagtc gccaaagaag
      121 gtcgagacga tggaagctcg ccctccaaag acgtagtcgt cgttgaacca cagttcgctc
      181 caagtctcga tggctttctt cacctcaggg ctcgagaagg gcagcgtgcc tgccacccag
      241 gcgtcgtaat tctccagcga cgtcgtgcgc agcatcatct cctcggtcca gtctgtcgct
      301 gtccagcctg tggctgcgcc actctcgatg ccgatacacc aggccgtgtc gccgtcgtcg
      361 gcgatcatct ggttaagcgc catcagatca tcccatgtct cgggaatctc gtagccagcc
      421 gcatcccagg ctgcctttgg ataccagacc aggctcttgc cattgaagcg gtggaataca
      481 cctgcctcca ctccctcaat agtggccata tcccgccagc tctggttgta ctgctggctc
      541 aaccactctt cggtcatcca ggaggtgaca tcaaccacct taccatcccg cacgaagtta
      601 gccatcttgc ccggctgcgg gaagtctaca atgtcaggcg catcgccggc ttcgacgcgg
      661 acggagatcg tggcttcgaa ttctttgttg ccaatgtagt tgacgttaat gccggtcagt
      721 tcttcgaact gagctactga gcggtccatc ttgacgccgt cggggtcgtt gccttcgaat
      781 gcgccgtcca ccgtcaccgt cgtccctgaa aactcgccgg tcaaggctcg ctccagaaaa
      841 ccacccggca tcatcgcgaa atctacctca ggcatcgccg gaggagcttc tgtcatctct
      901 ggggcttctg gctccacagc agccgggggc tctgtaggtg ttggttttgg agcacaggcc
      961 gccagcacaa acgacaggat aaaaaataca gatagtattg tggaaattgc tttccggttc
     1021 atcttctact ccttaatgta tagattgaat tgtctttgcc gaattttggc cgaactgatt
     1081 ttatctaatg agaaccacct cctttcatac tccgagacgt aatgaaggct gaactgatta
     1141 ttgaagtttc atccgtctat tactacgtga tgcaccacac gaatcacgca ctactaattg
     1201 ggtttccagg atgacctgct tgctctctgg ttcatctccg gctaacaact gggataatat
     1261 ttctgctgaa cggcgtgcca aatcacctgc cggaagacgg acggtggtca gtgggggatc
     1321 agagtagcgg gaaaagggga tgtcgtcgaa tccaaccaca gcgatatcct gtggaatacg
     1381 cagtcctcgc tcgcgaatag cagccaccgc gccatacgcc aaggtatcac taccgataaa
     1441 aactgcagtc attgtaacat tactcgccaa taggctgtgc atttgtcgat acccactctc
     1501 gatgtcaaag tctccgtagc ggatcaattc ggaatcaagg gctatcccgt tctctttaag
     1561 ggcctgttta tagcccgtta aacggtctaa ggcggccgta tatgaaatgg gagcattggt
     1621 aatgcaagca attcgagaat gacccaggtt aattaaatgc gccacggctg tattaaccgc
     1681 ggcgcggtta tccacgtcaa caaatgcaaa gtcgctatcg ggaatctgtc ccagcaaaac
     1741 agccggaaaa ttaacctcca gcaagctact taaggcatca tcatcataac gcgggccgga
     1801 taagatgatc ccatcaatgt gtttggcttg aataagctgt aaataagttt ctttctggtg
     1861 ctgaggctcg acgatatcga tgagcaaacg catgcccgat gggcggatgg tctctatcaa
     1921 cccatctaaa atttgggtta agaaggcatc agttgagatt tgatggggat tgcgaatcag
     1981 gattaagccg ataatgcgag cacgccggct ggctaatgcc tgggcagctg cttcggggac
     2041 atagttgagc tcacgtgccg catccagaac tcgccggcgg gtttggggac tgattttcac
     2101 gcccgggacg ttatttaaca ccaacgaaac cgtggtccgc gaaacaccgg ctttttcggc
     2161 aacatcctga ctggttatac gatggttggt caatcgaaat cctgggaatg tatctgtaac
     2221 taacacgcgt tagtaacgcg cgttaattat atattctgat tagataatag tcaatacaat
     2281 tattgtagat tcaccaaacg ggtgctagtt tgtcatgcca ctttcgacgc agccggggct
     2341 acggcatatt gaaatggagt gctaccgaca tactgttccg cagcctcaaa actactggtc
     2401 tgtgccagca gatcgatagc tgcccgatca tccagagaac gtgtcacctt tccatccacc
     2461 atttgaatga ccctgtccac atacttggtc atgcgaagat cgtgggtcac catgatccct
     2521 gcccggtttt gttcatggat caggtttgca aatgtctcta ccacctgaaa tgcccgttct
     2581 gtatctaaat tggctgtagg ctcgtcagcc agcacaactt cagggcgatg taccaaggcg
     2641 cgggcgatgg ctatgcgctg ttgttgccct ccagatagct ggtttggcag gctatttaat
     2701 cgctcctgca agcccaatcg agccaataat tccttggcgt gggcttttcc ttctttattc
     2761 agggttttat tgagacgcag catcaattcc acattctcta aagcagtcag gtagggcaca
     2821 aggttattag tctggaaagt aaaaccgatt ttttcccggc gaaatcttgt gcgttctttc
     2881 tctttcatct ctgccaacgc cacatccccg atgaaaatac ttcctgaagt tggagagagc
     2941 aaagcagcca gcatcgcaag catcgaagtc tttccagacc cactgggccc aaccagtgca
     3001 atgaattcac cgcgtttcac ctcaagtgag acctgatcca cagccacaac tgccgaggtg
     3061 tctttaccgt atattttcgt tatctcatcc gttcttagag ctatggtcat aaatatttct
     3121 tcccgttatt tgaagtcaga gagcgctgta gtttcaatta ctaagacagt ccaatggctg
     3181 tcagcgggtc tattttcagt gccaggcgaa tggataccag acctccaatt gggccaatga
     3241 agagcaacag tacggctgct gaaatcactg tcgacccggt aaagaggatg ggaatttcgc
     3301 tcggcatgcc cagcaccagc agcatcgtac ccaggctgcc gatgcccacg ccaatcaaag
     3361 taaccaggat gatctgcaac aaggttgcga aagccactgt agcgctcgag ctgccaattg
     3421 cttttagcac accaatttgc gctacctttt gtagcgtttg aatctggaag aaaccgccga
     3481 ctaccaatac gccgatcaac aaggtgaaga tttgctgcgt attcagcgtg ctctgctgag
     3541 ctgtatatcc tggcagtgct agaatggcag cctgcttatc ggccacctca atcccatcca
     3601 caaaatactc caacttttga gcgaccagat caaactgctg tggatcttga agtttcaccg
     3661 cggccaaatt gaaaataatc tcggatgtgg agctttcacc gtccccttga ggccgcacct
     3721 ttgaccaggt tcgaatgggc atgattgctg atggcaggaa aaaatactgg cgagagtcgg
     3781 taatacccac cacgctcagc tcatagaatt cttcctctgt gccttgaaca ctcttgataa
     3841 taaaccggtc tccgatggtc aagcccgccc gtgaagccag gtcttcatcg atgaccgcat
     3901 cattatttcg ctttgtgcgc aaacccgggc cagccatcaa gggcggatcg ccaggcttgc
     3961 ctggttctac ccccaccaag gcgatgtcaa tcggggatcg cccgtcggca aaaaccacgg
     4021 tcgccgtagc gagaccgaca gggccgacat ctgcaacgcc gtcaatgcgg cggatatcgc
     4081 tcagctttga ttgtcccaag cgactggcgg tcagttgtag atcgacatta ttttggtaga
     4141 tcagcatctc gccatccagc ttttcaatga gctgtttatt ggcattcgcc aaccccccag
     4201 ccagcgcagc gatgaacaac acaagaatgg taatcagtgc gataaccatg ctgaacaata
     4261 agtaccgccc acgcaagcgc caaatttctt tcaatgctag gtaaaaggac att
//
LOCUS       DBNHOH010000574         2665 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1636156, whole genome shotgun sequence.
ACCESSION   DBNHOH010000574 DBNHOH010000000
VERSION     DBNHOH010000574.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1636156"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1395
                     /locus_tag="AC16V7_09980"
     CDS             <1..1395
                     /locus_tag="AC16V7_09980"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6257488.1"
                     /translation="DFIPIEIYNRALTPEEWIRVISENGTAIMRLIYRQDPIDTDLIA
                     DIMEGGELTITELEIFQIGDFQIRLRTEGRGSTILLDKEGNLLGLNNPHLVPGAVVGQ
                     PLDRGTFPGLDGALTAAFNGEVDPERIFITLEPHERFYFAIPVKNEDDQEVLGVVIIY
                     IEHLPTANDIPETLTILLGQSVLILLLAAGLVGTIFGSLTARGMVLRLRRVSQVTDAW
                     SQGDFSEFIEDPTGDEISTMAVRLNQMAEQLQQFLKRSQEMAVSEERNRLARDLHDSA
                     KQEALAASFHLGTALTLFERDPQSAKNHLVKADTLVDSVRVELTDLIHELRPPSMNGA
                     RFDETLNEYVIEWAHQTGIEATLDVDGFVDLSLEVKQAIYRIMQEALANVAKLSSAKS
                     VDVTMRFGNYSVEFCISDDGKGFGTQQPHDGIGLDSMRERVESLKGDFSIQSEPGQGT
                     KVCLTIPTEGWRRP"
     gene            1396..2049
                     /locus_tag="AC16V7_09985"
     CDS             1396..2049
                     /locus_tag="AC16V7_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6257489.1"
                     /translation="MQEPITVMIVDDHEMVRSGAKGYLEAQPEIAVVAEAESGIEAVR
                     LAREYIPDVVLMDLVMPGMDGVEATRKVKDISPRTQIIILTSFHEDEHIFPALQAGAI
                     SYLLKDVKAQELVEAIQRASKGEATLHPRVAARLIKQFSHGEPERSTLFTELTEREVE
                     VLTLIARGFTNQKIADELVISIGTVKGHVSNILSKLHLADRTQAAVYAWQEGIVRRE"
     gene            2292..2573
                     /locus_tag="AC16V7_09990"
     CDS             2292..2573
                     /locus_tag="AC16V7_09990"
                     /inference="COORDINATES: protein motif:HMM:NF025601.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HIR6257490.1"
                     /translation="MDTTQNTNANDKSNWTSIGSAVREILRESRNRRVVIRKASGKKV
                     IDCSVLLALILSIGAPVLPAFVILGVLVESISVSFEKRGVQISTKETIQ"
BASE COUNT          703 a          610 c          699 g          653 t
ORIGIN      
        1 gattttattc cgatcgagat ctataatcgc gcgctcacgc ccgaagaatg gattagggtt
       61 atcagcgaaa atggcactgc cataatgcgc cttatttaca ggcaagaccc catcgataca
      121 gatttgatcg cagacataat ggaagggggt gaattaacca ttacagagct cgaaatattt
      181 caaatcggcg actttcaaat ccggttaagg acagagggac ggggaagcac tattctcctc
      241 gataaagaag gaaatctgct tggactcaac aatccgcatc ttgttcctgg agctgtagtc
      301 ggacaaccgc ttgatagggg gactttccct ggtctggacg gtgccctgac agcagccttt
      361 aatggagaag ttgatccaga gcggatattc atcacccttg aaccacacga acgtttttat
      421 tttgcgatcc ctgtaaagaa tgaagatgat caagaggttt tgggggttgt gatcatttac
      481 atagagcatt tgccaaccgc caatgatatc ccagaaaccc taaccatatt gttgggacag
      541 agtgtcttga tcttgttgct agctgccggt ctggttggaa ctatttttgg ctccctgacg
      601 gcccgtggta tggttctgcg cttacgacga gtttcgcagg tgaccgatgc ctggagccaa
      661 ggcgattttt ctgaatttat cgaggatcct actggcgatg aaatcagtac aatggcagta
      721 cgtctcaacc aaatggcgga acaactccag cagttcctca aaagaagcca ggagatggct
      781 gtttctgaag agcgcaaccg cctggcgcgc gacctgcacg acagtgccaa gcaagaagcc
      841 ctagctgcct cctttcattt ggggacggcc ttgacgcttt tcgagcgcga tccccaatcg
      901 gcaaaaaacc atttggtgaa agccgatact ctggtggatt cggtgcgggt ggagttgacc
      961 gatttgatcc atgaactgcg acccccgtca atgaacggcg cgcgttttga tgaaaccctc
     1021 aatgaatatg tcattgagtg ggcccaccaa accggtatcg aagcgaccct ggatgtggat
     1081 ggattcgtcg atttatctct ggaggtcaaa caggccattt accgaatcat gcaggaagcg
     1141 ttagcgaacg tggccaaact gagctcagcg aaaagcgttg atgttacaat gcgttttgga
     1201 aattattcag ttgaattctg catcagtgat gatggcaaag ggtttggcac ccaacaaccg
     1261 catgatggta taggacttga ttcgatgcgt gagcgggtgg aatcgctcaa gggtgatttc
     1321 agcatccaaa gcgaacctgg gcaaggaacg aaagtctgtc taacgatccc aaccgagggg
     1381 tggaggaggc cgtaaatgca agaaccaatc acagtcatga tcgtggatga ccatgaaatg
     1441 gtgcgcagtg gggccaaagg atacctggaa gcgcagcctg agatcgcagt ggtggcggaa
     1501 gccgaatcgg ggatagaggc tgtgcgcctg gcccgcgaat atattccgga tgtcgtactt
     1561 atggacctgg tcatgcccgg catggacggt gtggaagcca cccgcaaagt gaaggacatc
     1621 agcccgcgca cacagatcat catactgacc tccttccatg aagatgagca tattttcccg
     1681 gcgctccagg ccggtgcgat ttcctatttg ctcaaggatg tcaaggctca ggaattggtc
     1741 gaagccattc aacgggcctc caaaggggag gcaacactgc atcccagagt tgcggcccgc
     1801 ctgatcaagc aattcagcca tggagaacct gaacgtagca ccctttttac agaactcacc
     1861 gagcgagaag tcgaagtgct gacattgatt gccagaggct ttactaacca gaaaatcgca
     1921 gatgagttgg tcatcagcat tggcactgtc aaaggccatg tcagtaatat cttgagcaaa
     1981 cttcacctcg ccgatcgcac ccaggccgca gtttacgcct ggcaggaagg tattgtccgg
     2041 cgcgagtaat cctggctttt accctacttg cgatatattt ctctgggggt aatttaatag
     2101 ggcttttcca gtaccgcagt aattaaatat tcaatcttga gcttggatta agaacctttc
     2161 cgtaggctgg ggcaaaacat acactatggg tcgtgtgatt ctttctcctg catgctattc
     2221 tgtatgtgcg tttgtaagtg tgttgtacta agcacaagaa aaaggcaaac aaaagatcag
     2281 gagaaattat catggatacc acccaaaaca caaatgcaaa tgacaaatcc aattggactt
     2341 caatcggtag tgctgttcgt gaaatcctac gggagagtcg caatcggcgc gtcgtgatca
     2401 ggaaagcttc tgggaagaaa gtcatcgatt gttcggtgtt actggcattg attttatcca
     2461 tcggtgcacc tgttcttccc gcctttgtga tcctaggcgt gttggttgag tcgattagtg
     2521 tttcgttcga aaagagggga gtacaaatat caacaaagga gacaatccaa tgaataaagt
     2581 aacgaactgg atcaagagtc atcaagttgc agctttcttt atcttggttt atgtgatagc
     2641 ttggccgggc atgttcctgg tctac
//
LOCUS       DBNHOH010000575         2583 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_546217, whole genome shotgun sequence.
ACCESSION   DBNHOH010000575 DBNHOH010000000
VERSION     DBNHOH010000575.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_546217"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..1359
                     /locus_tag="AC16V7_09995"
     CDS             226..1359
                     /locus_tag="AC16V7_09995"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003251530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR6257491.1"
                     /translation="MSEVFIISAVRAPIGLGRPTGALAPLAPVDLSAMILCEAVRRAG
                     VEPAWVEDVIWGCVTPLGDQGANLARLVALKAGFPVRVPAVTLNRMCGSSQQAVHFAA
                     QAILSGDMQIVIAGGTEMMSHQTIGSDWPQQWPDDFPYKLVHQGISAEMMAEIWRLTR
                     EQLDDYAYQSHLRAMRAIQCGYFESQILPVSLPDNRLVALDEGVRMPPDRDKMSSLKT
                     VFKENGVITAGNASQISDGCAALVLASDKAVRRYNLTPRARVVARLVIGSDPVLMLDG
                     PISATRQIVEKAGLSLDDIDVIEINEAFASVVLAWASEMNPDMQRVNPNGGAIALGHP
                     LGATGAILMTKLVHELDRSGGRYGLQTMCIGHGMATATIIERV"
     gene            1374..1871
                     /gene="folK"
                     /locus_tag="AC16V7_10000"
     CDS             1374..1871
                     /gene="folK"
                     /locus_tag="AC16V7_10000"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016330205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIR6257492.1"
                     /translation="MNKTHSAFVALGSNLGDRLGNLQAAIGLMPPALHVKEQSPIYET
                     SPWGYADQPDFLNQVVHAETDLAPLELLAYIKSIEVTLGRTPTFVYGPRQIDLDILFY
                     DDLVLQTQELIIPHPHLASRAFVLCPLADIAPDLRHPIQGQTVLQLLEGVDRQGIRLL
                     RANAS"
     gene            2016..2441
                     /locus_tag="AC16V7_10005"
     CDS             2016..2441
                     /locus_tag="AC16V7_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257493.1"
                     /translation="MSEEKLKPLKWKPDPDVGYTVVRRQDGGMHITFTDVSHTTLEHW
                     REFAMEHLLNSDRLTRNLYDLRQIDELPEEAVQYAIEVNNDPSVRNIRLAVVATHDSV
                     KEALKRIAALTTSGGVEMGLFSTLEDADVWLNRPLTLLV"
BASE COUNT          569 a          687 c          692 g          635 t
ORIGIN      
        1 ttcggttgaa aatgtgctat ccttcatagg ggatgctcct ttcggtaagc gcggttgtga
       61 gaacctactt taccgaatta gcatcctttt ttcaatacct ttctccaaaa ggccaaactc
      121 gagcccattt tctctcctca gtggtgcgaa atcgcccaaa tgcgattgcc ctacgattct
      181 caacgatcca agtatctttg agatgagttc tagtatactt aatatatgag cgaagtattc
      241 atcatctctg cggtgcgcgc gccgattgga ttgggcaggc cgactggcgc gctggctccc
      301 ctggccccgg tggatctgag cgccatgata ctttgcgaag ctgtccggcg ggctggcgtc
      361 gaacccgcct gggtggagga tgttatttgg ggctgcgtga ccccgctcgg tgaccagggc
      421 gctaacttgg cccgcctggt ggctctcaag gctggtttcc ctgtacgcgt gcccgccgtc
      481 acgctaaacc gcatgtgcgg ctctagccag caagctgtgc actttgctgc ccaagccatc
      541 ctctccggcg acatgcagat tgtcatcgcc ggcggtacgg agatgatgtc gcaccagacc
      601 atagggagcg attggccgca acagtggcct gatgatttcc catacaagct ggtgcatcag
      661 ggtatcagcg ccgagatgat ggcggaaata tggcgcctga cccgtgaaca actggatgat
      721 tatgcttacc aaagccacct gcgcgccatg cgcgccatcc agtgcggtta ctttgagagc
      781 cagattttgc ctgtatccct acctgataat cggctggtcg cactggatga gggcgtgcgc
      841 atgccgcccg acagggacaa gatgtcttcc ctaaagacag tatttaaaga aaatggggtc
      901 attactgctg gcaatgccag ccagatcagc gatggctgcg ccgccctagt gctggcctcg
      961 gacaaagcgg tgaggcgcta caatctgacc ccgcgcgcac gggtggtggc tcgtttagtg
     1021 attggctcag acccggtttt gatgctggat gggccaattt ctgccacacg ccagatcgtc
     1081 gagaaggccg gcttatcctt ggatgatatc gacgtaatcg agatcaatga ggcttttgct
     1141 tcagttgtcc tggcctgggc atccgagatg aatcccgaca tgcaacgcgt caacccaaat
     1201 gggggcgcta ttgccttggg gcatccgtta ggtgcaaccg gcgccatttt gatgaccaag
     1261 ctggtgcatg aattggatcg tagcgggggt cgctatgggc tgcagacaat gtgcatcggt
     1321 cacggtatgg caaccgcgac gatcatcgag agagtttaat tttgttgcct gcagtgaata
     1381 aaacacattc cgccttcgtc gcgctgggta gcaacctggg tgaccggctg ggaaatttgc
     1441 aagcagctat agggctgatg cctccagctt tgcatgtgaa ggagcaatcg cctatctatg
     1501 agacatcccc gtggggctat gcagatcagc cggatttcct caaccaggtt gttcacgccg
     1561 agaccgatct agcgccctta gaattgttgg cttacattaa gtccatcgag gttaccctgg
     1621 gacgcacacc taccttcgtt tatggaccgc gccagatcga cctggatatc ctgttttacg
     1681 atgacctggt gctgcaaacc caggagctga taatccccca tcctcacctg gcatcgcgcg
     1741 cttttgtact ctgccccctg gctgacattg ctccagactt gcgccaccct atacaagggc
     1801 aaacggtgtt gcaactactg gaaggcgtgg atcgccaggg cattcgactg ctgcgagcga
     1861 atgcctcctg agcaagaaat gtgacatgtg tccgtttgaa ttcattttct tatcatatac
     1921 ttggacaaaa tttaggatag aatatcttta cgactcgcat gggagatctt ttctcggtta
     1981 atattcaatt cataaccgag ttgtggaggc aagtcatgag cgaagaaaaa ttaaagcccc
     2041 tcaagtggaa acccgatcct gatgtgggct atacggttgt ccgccggcag gatggcggta
     2101 tgcacattac ttttaccgat gtctcgcaca ctacactgga acactggcgc gagtttgcta
     2161 tggagcactt gctcaactcc gaccgcctga cacgtaacct ctacgatctt cgccagatcg
     2221 acgagttgcc tgaagaagct gttcaatatg cgattgaggt gaacaacgac ccctcggtgc
     2281 gcaatatccg cttggcggtc gtagccaccc atgattctgt gaaagaagcg ctcaagcgaa
     2341 ttgcagcttt gaccacatcc ggtggcgttg agatgggcct attcagcact cttgaagatg
     2401 cagatgtctg gctaaaccgt cctctcacgc tactggttta atgcataatt ctctcaccat
     2461 aggaaattca acttttacat ggggagcccg gacctacgtc atgggtatcc tcaatatcac
     2521 gcctgacagt ttttcaggcg atgggctgct ttcctcaata tctgatacag gggataagtg
     2581 gct
//
LOCUS       DBNHOH010000576         3573 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_932289, whole genome shotgun sequence.
ACCESSION   DBNHOH010000576 DBNHOH010000000
VERSION     DBNHOH010000576.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_932289"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /locus_tag="AC16V7_10010"
     CDS             <1..1203
                     /locus_tag="AC16V7_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR6257494.1"
                     /translation="EGIYFWDADGKRYIDFSSQLMNMNIGHQHPKVVGAIQEQASQLC
                     FVHPGLATEARGKLGKKISEVAPGHLNKTFFCLGGAEANENAIKLARLYTGRNKILAR
                     YRSYHGATHGAMALTGDYRRLAVEPTMPGAVHFLDPYCYRCPFGWTRETCHRECISHI
                     EEIIQYEGPDHIAAIIMEGVTGSNGLIVPPDDYWPRVREICDKYDILLISDEVMSGWG
                     RTGKWFAVDNWEVVPDMITTAKGLTSGYVPLGAVIVSDDIAEYFEDKMLWAGLTYSGH
                     ALACAAGLATLEVYQEDGLIENAVKVGRYLGERLEAIKLSHPSVGEVRYIGLFSALEI
                     VKNRETKEPIDPLTEVAKYLKEHGLFTFVFHNMLFIVPPLCITEAQLDEGLEIIEQGL
                     LITDAQTR"
     gene            1225..2574
                     /locus_tag="AC16V7_10015"
     CDS             1225..2574
                     /locus_tag="AC16V7_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR6257495.1"
                     /translation="MPQSLDEALTYSNRWIDIIHKTEIPLDEGKQIIEESKNNFAEHF
                     NKGWLEYRKSVTEAGDWAATEWTGSGAVFRDLIGREYIDCLGGYGMMDLGWGHPDVVA
                     AVRAQLNRTPMPSQELIDPLRGVLARLMANITPGDLKYSFFAGSGTESIEGAIKLAKM
                     YTKKNGFIVAVKAFHGKTLGSLSMMGKFDFRQPPGILYGGPIYHVPFGDADAVEQQLD
                     ICKKVGVDLAAVLFEPIQGEAGAIVPPDDFWPRVRELTKHYGVLLIADEVQTGMGRTG
                     KLWGLDHWGVVPDILAVAKSLGGGVMPVSAFCSTEEIWQCMMYPNPFIHTTTTGGGAL
                     ACSAAIASIHVTLRDRMWEQAAEKGDYLIPKLKELAARYPQIFEDITGKGLLIGMHFH
                     DPETGYKIASGLFKRGVLVAGTLTSAQTIRIEPPLVITYDQIDTVLNRLEDTLREVS"
     gene            2663..>3573
                     /locus_tag="AC16V7_10020"
     CDS             2663..>3573
                     /locus_tag="AC16V7_10020"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019057045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HIR6257496.1"
                     /translation="MKVLLVGVGGVGEAIAAIAKNRSWVEQIVLADYNLERSREVQAR
                     LNDKQRFPVEWVDASQQDQIEGLASKYQVDMIMNACDPIFNVPIFEAAYNYGCNYMDL
                     AMTLSEPHPEKPYELCGVKLGDYQFERAGLWEKKGLLALVGLGVEPGMANVFARYAQD
                     YLFDEIDEIGVRDGANIEVRGYEFAPNFSIWTTIEECLNPPVIWEKKRGWFTTEPFSE
                     PEIFEFPDGIGSIEVVNVEHEEVLLIPRWVKCNRVTFKYGLGDQFIDVLKTLRMLGLD
                     NKEPIKVKDVMVAPRDVVAACLPDPAH"
BASE COUNT          899 a          714 c         1031 g          929 t
ORIGIN      
        1 gagggtatct atttttggga tgcagatggt aagcgttata ttgatttttc atctcagctg
       61 atgaacatga atatcggcca tcagcacccc aaggtggtgg gggctatcca agaacaagcc
      121 tcacaattgt gctttgtgca ccctggactg gcgacggaag cgcgaggaaa gctggggaaa
      181 aagatttccg aagttgcccc gggacacttg aacaaaacct ttttctgttt gggtggcgca
      241 gaagccaacg agaatgccat caagctcgct cgactgtaca caggtcggaa taaaatcctt
      301 gcgcgctatc gttcttatca tggcgcgacg cacggagcga tggctctgac aggtgattat
      361 cgccgactgg ctgttgagcc gacgatgcct ggtgctgtac atttcttgga tccttattgt
      421 tatcgctgtc cttttggatg gacgcgggaa acctgccatc gagaatgtat cagtcatatc
      481 gaggagatta tccagtacga aggacccgat cacatagcag ctattatcat ggagggggtg
      541 actggttcga atggactgat cgtccctcct gacgattact ggccgcgggt aagggaaatc
      601 tgcgacaaat atgatatttt gctcatttct gatgaggtca tgagtgggtg ggggcgaacc
      661 ggcaaatggt ttgcagtaga taactgggaa gtcgtgcccg atatgatcac aacagctaag
      721 ggtttgacat ctggatatgt tcctttgggt gctgtgatcg tctcagatga tatcgcagag
      781 tattttgaag ataagatgct ttgggccggg cttacctata gcgggcatgc cctggcatgc
      841 gcggctggac tggcgacttt ggaagtgtat caggaagatg gcttgatcga aaatgctgtc
      901 aaagtaggtc gctatttggg cgagcgcttg gaagctatta agctgagtca tccatcagtg
      961 ggggaggtgc gctatatcgg actattctca gcattggaaa ttgtcaaaaa tcgagaaacc
     1021 aaagagccaa tcgatccctt gactgaggta gccaagtatc tcaaggaaca tggcttattt
     1081 acatttgtct ttcataatat gcttttcatc gtaccgcctt tatgtattac agaagcgcag
     1141 ctcgatgagg gcttggagat tattgagcag ggattgctaa ttaccgacgc acagaccagg
     1201 tagatatata tacggagcat atttatgcct caatcgcttg atgaagcatt aacctattcg
     1261 aatcgatgga tcgacattat ccataaaacc gagatccccc tggatgaggg caagcaaatt
     1321 atcgaggaaa gtaagaataa ttttgctgag cattttaata aggggtggct ggaatatcgt
     1381 aaatcggtaa ccgaggctgg cgattgggca gctacagaat ggactggctc aggtgcagtg
     1441 tttcgagacc tgatcggtcg cgagtacatt gactgcctag gggggtatgg catgatggat
     1501 ttggggtggg gacatccaga tgtcgttgcc gcagtgcgtg cccaattaaa tcgcactccg
     1561 atgcccagcc aggaattgat tgatccattg cggggcgtac tggcgagatt gatggcaaat
     1621 attactcctg gtgatctaaa gtacagcttt ttcgcaggta gtggaacgga gtccatcgaa
     1681 ggggccatca agctggcaaa aatgtatacc aaaaagaacg ggtttatcgt ggcggtcaag
     1741 gcttttcatg gcaagacctt gggatcgttg agcatgatgg gcaagttcga tttccgccag
     1801 ccaccgggga ttctatatgg tggaccgata taccatgtgc cctttggtga tgcagatgct
     1861 gtagagcaac agcttgatat ttgtaagaag gtgggcgtcg acctggctgc ggtgttgttt
     1921 gaacccatcc agggagaggc cggggctatt gttccaccgg acgatttttg gccgcgcgtg
     1981 cgcgagctga ccaaacatta cggcgttttg ctgatcgccg atgaggtcca aaccggtatg
     2041 ggacgcacgg gaaagctttg gggcttagat cattgggggg tagtgccgga tatccttgcg
     2101 gtagcgaaat cgttgggtgg aggggttatg ccagtgagcg cgttttgctc gaccgaggag
     2161 atttggcagt gtatgatgta tcccaatccc tttatccata ccactactac tggagggggt
     2221 gccttggcgt gcagcgcggc gatagcctcg atccatgtca cattgcgcga tcgaatgtgg
     2281 gagcaagctg ctgaaaaggg cgattatctc atcccaaaat tgaaagaact ggctgcgcgt
     2341 tatcctcaaa tttttgagga tattactggc aaaggcttat tgatcggcat gcattttcat
     2401 gatccggaaa cgggatataa gattgcatct ggtctattta agcgcggagt gcttgtggct
     2461 ggaacgctga ccagtgctca aaccatacga atcgagccgc ctctggtgat cacctatgat
     2521 cagattgaca cggtactgaa ccggctcgag gatacactaa gagaggtgag ttaagtgcaa
     2581 tgagtttgaa cggatatttt ttattttttg gagctatgcc tctagagaca ttagctgaga
     2641 gcatgaaaat cggagggaga atatgaaagt tcttttggtc ggtgtcgggg gggttggaga
     2701 agctattgcc gccattgcta aaaatcggtc ttgggtcgag cagattgtgt tggcagatta
     2761 caatctggaa cgatcgagag aggttcaagc gaggttgaat gataagcaac gattcccggt
     2821 tgaatgggtg gatgcttcgc aacaagatca gattgaaggg ttggcgagta aataccaagt
     2881 agatatgatc atgaacgcct gtgatccaat cttcaatgtg cccatttttg aggctgcgta
     2941 caactacggc tgtaattaca tggatttagc gatgacgcta tctgaaccgc acccagaaaa
     3001 accctatgag ttatgtggtg tgaagctggg tgattaccag tttgagcgtg caggcttgtg
     3061 ggagaagaag ggcctgctgg ccttggtggg cttgggtgtg gaaccaggaa tggctaatgt
     3121 gtttgctcgc tatgctcaag attacctctt cgatgagatt gatgagatcg gagtgcgtga
     3181 tggcgcaaac attgaagtgc gcggttacga atttgcccca aatttctcca tctggacaac
     3241 gatcgaagag tgtttaaatc cgccagtcat atgggaaaag aagcgaggct ggtttactac
     3301 cgaaccattc tctgagccgg agatattcga atttcctgat ggaattggtt cgatagaggt
     3361 cgtcaatgta gagcacgaag aagtgttgct tatacctcgc tgggtcaagt gtaaccgtgt
     3421 aacctttaaa tatgggcttg gcgatcaatt tattgacgtg ctcaaaacgc tacgtatgtt
     3481 gggattagac aacaaggaac cgatcaaggt taaagatgtt atggtagcgc cgcgagatgt
     3541 ggtcgcggca tgcttgcctg atccggcaca tat
//
LOCUS       DBNHOH010000577         2087 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_226495, whole genome shotgun sequence.
ACCESSION   DBNHOH010000577 DBNHOH010000000
VERSION     DBNHOH010000577.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_226495"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="AC16V7_10025"
     CDS             complement(<1..415)
                     /locus_tag="AC16V7_10025"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR6257497.1"
                     /translation="MDRDQFIQIMHSAKLYDLTQDCSIFTPPWPGEKSLEVHFFKRVT
                     GAYGGGPGANGQILNWSNTVGTHLVGETAFHSGGRSIANIPLKDLCGPGVVVDISDAV
                     SDYSLYTPEMITSRANVKKGDILIINTGYHRYSWDW"
     gene            complement(605..1846)
                     /locus_tag="AC16V7_10030"
     CDS             complement(605..1846)
                     /locus_tag="AC16V7_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbE family protein"
                     /protein_id="HIR6257498.1"
                     /translation="MDIEKANAETFQRMVEARPMLVGMGKAINVIPGMRDNLLMHAGP
                     PITWERASGPMQGAITGALIFEGKAKSEAEAQELVESGKIELEPCHHHQAVGPMAGII
                     SPTMSVYILENKTHGNNSYSNLNEGYGKVLRYGAYSEEVLDRLRWMEDVMAPVLGKAI
                     ELSGGIDMRALLAEALHMGDEGHNRNKAGSILFTKSLAPHIVKSTNDSDIAAKIIQFL
                     GDNALSVLNPVMAACKGMVDAAHGIEGSTIVSVMARNGTDFGIRVSGLGDQWFTAPVA
                     IPQGLYFPGFTATDASGDIGDSTITETAGIGGFAMASAPAIVTFVSGTPKDATNATLE
                     MYEICYSEHKYFTIPTLNFRGTPTGIDIRKVVETGITPRVNTGIAHKNAGVGQVGAGL
                     VRPPMAMFEQAVMAFAEHYGF"
BASE COUNT          471 a          551 c          509 g          556 t
ORIGIN      
        1 gccaatccca ggagtatcgg tgataacccg tattgatgat caggatatcc cccttcttga
       61 cattggctct cgaggtgatc atctcgggag tgtaaaggct atagtcagac accgcatcag
      121 agatatccac gactacgcct ggtccacata aatccttaag tggaatattg gcgatggatc
      181 tacccccgga atgaaaggcg gtctctccaa ccaggtgtgt tccaacggta ttgctccaat
      241 tcaagatctg tccattagct cccggtccac caccataagc gccggttacg cgcttgaaaa
      301 agtggacttc gagtgatttc tcaccaggcc aaggcggggt gaagatgctg caatcctggg
      361 ttaagtcata tagtttggcg gaatgcatga tttggataaa ctgatcgcga tccattattt
      421 gcctccactt cttttgtgtt catccagata aagtaatttt tgatttctat tagctatggc
      481 gtcatctagc ttagaactgt tgcgaccgga atgtacgcat tcgtcgcaaa tcacaagacg
      541 atggttgaga agcgcggcca taatctctct gtttgacaga atggataatc aggtcgcctc
      601 agggttaaaa accgtaatgc tcagcaaagg ccatcacagc ctgctcaaac attgccattg
      661 gggggcgtac taatcccgct cccacctggc ctacaccggc atttttgtga gcgatccccg
      721 tattcacccg tggagtgata ccagtctcca ccaccttgcg gatatcaatt ccggtgggag
      781 tcccccggaa gttcaatgtg gggatggtga agtacttgtg ttcagagtag cagatctcgt
      841 acatttccag ggtggcgttc gttgcgtctt tcggtgtgcc gctgacaaag gtaacgatag
      901 caggcgcgga tgccatggcg aatccaccga tcccggctgt ttctgtgatg gtgctgtctc
      961 cgatgtcacc gctagcatcc gtggctgtga atcccggaaa atagaggcct tgcgggatgg
     1021 ccaccggagc ggtgaaccac tggtctccta acccgctcac gcggatgccg aaatcggtgc
     1081 cgttgcgagc catgacgctg acaatcgtgc tcccttcgat gccatgcgct gcgtctacca
     1141 tgcctttaca ggctgccatt actggattga gaacgctaag ggcgttatca cctaagaatt
     1201 gtatgatttt tgccgctata tcgctatcgt ttgttgactt gacgatgtgt ggcgccaggg
     1261 attttgtgaa taaaatcgac ccggctttat tgcggttatg cccttcgtca cccatgtgca
     1321 acgcttcagc cagcagcgct ctcatatcaa tcccgccgct caactcgatc gctttaccta
     1381 gaacaggtgc catcacatct tccatccagc gcaagcgatc caagacttct tcgctgtaag
     1441 caccatagcg cagcacttta ccataccctt cgttcaggtt ggaataggaa ttgttgccat
     1501 gggttttgtt ctctaagata tacacagaca tcgttgggga tataatacct gccataggac
     1561 cgacagcctg gtgatggtgg caaggctcca gttcaatttt cccggattcg accagctctt
     1621 gtgcttctgc ctcgctcttg gccttgcctt cgaagatcaa agcccctgta atcgcgccct
     1681 gcattggccc agaggcgcgc tcccacgtga tcggcggacc ggcatgcatc agcagattat
     1741 ctcgcatacc ggggatgacg ttgattgctt tccccatccc caccagcatc ggacgggctt
     1801 ctaccatccg ttgaaaggtc tcagcattag ctttttctat gtccattttc tgcccttttg
     1861 ttataagaac catgcgattg tgatagagat ttggctgttg aactcaacaa tgccaatctc
     1921 aactacgatc taggaaagcg cttcccgggc tcaaatcgag atcgagccgt ttttagaatc
     1981 tcctcgtaaa tttttagatt gcgatcttcc aggtggtcaa aggtttgcaa gcttttgcta
     2041 ccccatgtga tatatcgaag cggatcggga tccagctttg cagcgat
//
LOCUS       DBNHOH010000578         1902 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_354554, whole genome shotgun sequence.
ACCESSION   DBNHOH010000578 DBNHOH010000000
VERSION     DBNHOH010000578.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_354554"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="AC16V7_10035"
     CDS             <1..412
                     /locus_tag="AC16V7_10035"
                     /inference="COORDINATES: protein motif:HMM:NF013173.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HIR6257499.1"
                     /translation="GQINAHYGSSQWEPVRLLVGDNYPRAVAALQQYDLLLVNAIADG
                     MNLVAKEGPIVNQKDGVLILSDRAGARQQLESGAIVISPCDVYATAEAMHQALVMPQG
                     QRYQYAERLRWMIEREDITAWLEHQLEAVDELNL"
     gene            425..1672
                     /locus_tag="AC16V7_10040"
     CDS             425..1672
                     /locus_tag="AC16V7_10040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6257500.1"
                     /translation="MFKPRIGILHYTAPPVVGGVEAVIQAQASLFNRMEYPVSVVAGR
                     GEASILPPGTSLVLLPLLDSQHEEILHINAYLENGDVHPGFDSMVEKIAQALQPVVEE
                     VDHLIIHNVFTKHFNLALTVALDQLIEAGSIRHAIVWCHDISWTSPSSRSKVFPGYPW
                     DILRTYRSDVTYVTVSAHRQKALAKLFECSLHKIHLIYNGVEPSSLLGLSPEGLRLAD
                     RLNLFGSSLILLMPVRVTRAKNIEYALHVVAALKQEVSTKLVLTGPPDPHDPENMRYF
                     HSLLRLRQKLGVESEARFIFEEGYQPDEPYLITERVVGDLFRLCDVVFMPSHREGFGM
                     PVIEAGLSGVPVICTDIPAAQEIGQKEVTIFSKEQSPSDVARVIIEIVEANPVSRFRR
                     RVRQNYTWEALFQRQIEPILKGQ"
     gene            1680..>1902
                     /locus_tag="AC16V7_10045"
     CDS             1680..>1902
                     /locus_tag="AC16V7_10045"
                     /inference="COORDINATES: protein motif:HMM:NF014418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HIR6257501.1"
                     /translation="MKTQPAQQALTRLVNFERLWLFLDYDGTLAEFAPSPDIVIPDPE
                     LLDLLTRLANKPDLRLAVISGRRLQHIRKL"
BASE COUNT          492 a          460 c          469 g          481 t
ORIGIN      
        1 cggtcagatt aatgcgcatt acggtagcag ccagtgggaa ccagttcgtc tgctggtagg
       61 tgacaactac ccgcgagcag tggctgccct acaacagtac gacctcttat tggttaatgc
      121 catagcagat ggtatgaacc tggtcgcaaa agaaggacca atcgtcaatc agaaagatgg
      181 agtactgatc ctttcagata gagccggcgc ccgacagcag cttgagtcag gtgctatcgt
      241 catctctcca tgcgatgtgt acgctacggc agaagcaatg caccaagccc ttgttatgcc
      301 gcaggggcaa cgctaccagt atgctgagcg tttgcgctgg atgatcgaac gtgaggatat
      361 cactgcttgg ctagaacatc agcttgaggc agtggatgag cttaatctat agtacgagcg
      421 tcttatgttt aaaccacgta ttgggatttt acattacact gctccacctg tcgtcggcgg
      481 tgtggaggca gtgatccaag cacaagcaag cctatttaat cgaatggagt atccggtctc
      541 tgtggtcgca gggcgcggtg aggcctccat attgccaccc ggcacctcgt tagtgctcct
      601 tccgttgctt gacagccaac acgaagaaat attacacatt aatgcttacc tagaaaacgg
      661 tgatgtgcac ccaggttttg actcaatggt tgaaaagata gctcaagctc ttcaaccagt
      721 ggtagaagag gttgatcacc tgatcatcca caatgttttc accaaacatt tcaacctggc
      781 tttgaccgtt gcgctagacc agctgatcga ggctggctcc attcgtcacg ccattgtttg
      841 gtgccatgat atctcctgga cgagcccaag ctcgcgctca aaagtattcc ctggatatcc
      901 atgggatatc cttcggactt accgttcaga tgtaacctat gtaacagttt ctgcacaccg
      961 ccagaaggct ttggcaaagc tgttcgaatg cagcctgcac aaaattcacc tgatctacaa
     1021 tggcgttgaa ccttccagcc tgctcggtct atccccggaa ggacttcgat tggctgatcg
     1081 cttgaactta tttggctcaa gcctgatact tttgatgcct gtcagggtaa cacgcgcaaa
     1141 aaacatcgag tatgccctgc atgtggttgc ggcgctaaag caagaagtga gtacgaaact
     1201 ggtactgact ggcccgccgg atccgcacga ccctgagaat atgagatatt tccactcatt
     1261 gttgaggttg cgacagaaac tcggcgtaga aagcgaagcg cgcttcatat ttgaagaagg
     1321 atatcaacct gacgaaccct acctgattac cgaacgcgtg gtaggcgatt tatttcgctt
     1381 atgcgatgtt gtctttatgc ctagccatcg cgaaggattt ggcatgcctg ttatagaggc
     1441 tggtttaagt ggtgtgcctg taatatgtac tgatatacct gcggctcaag aaatcggtca
     1501 aaaggaagta accatctttt ccaaagaaca atcacccagc gatgtagcaa gggtaatcat
     1561 cgagattgtg gaagcaaatc cagtgtcgcg ctttcgaagg cgggtaaggc aaaactatac
     1621 ttgggaagcg cttttccaac gtcaaattga accaattttg aagggtcagt gaaagactta
     1681 tgaagactca accagctcag caagcattga cccgactggt taatttcgaa cgactttggc
     1741 ttttcttgga ctatgacggc actctggctg aattcgctcc ttcgccagat atcgtgattc
     1801 ccgatccaga gcttctcgat ttgctaacaa gattggcaaa taagcccgac ctgcgtttgg
     1861 cagtgatcag cggtagaaga cttcaacata ttcgtaaact cg
//
LOCUS       DBNHOH010000579         1647 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1701595, whole genome shotgun sequence.
ACCESSION   DBNHOH010000579 DBNHOH010000000
VERSION     DBNHOH010000579.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1701595"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="AC16V7_10050"
     CDS             <1..461
                     /locus_tag="AC16V7_10050"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HIR6257502.1"
                     /translation="QIKNELYSAYLYLSMSSYFEASNLPGFAKWMRLQSEEELEHALK
                     FYDYVNERGGRVTLFEIPQPKSDWKTSLEVFNQVLEHEQHVTSLIHKLYETALSEKDY
                     ASQVMLQWFIDEQVEEEKNATEIVEQLKLIDVHGTAVLMLDHRLSKRGKE"
     gene            592..1218
                     /locus_tag="AC16V7_10055"
     CDS             592..1218
                     /locus_tag="AC16V7_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257503.1"
                     /translation="MGNIRIEKLAPLSGVISLLLFCIGAALLGIYEYLPIADKLKAIL
                     DTNASSIFTGGYLGSISAFFMIWFAGSVYSALREQEGGTGRLSMVAFGGGVASGVALA
                     SGFSAILTSGARAGAAGGITAIEAVTLYDLYSQVLGGMFAITMAVFIGATGTVSLRTA
                     MFPSWFGWVSVLIAIGLLTPFAYAVLALVLIWLIIVSFWLYRRGLSTT"
BASE COUNT          441 a          389 c          336 g          481 t
ORIGIN      
        1 aacagatcaa aaatgagcta tattcagcct atctctattt gtccatgagt tcttacttcg
       61 aagccagcaa tcttcctggt tttgctaaat ggatgagact tcaatctgaa gaagaacttg
      121 aacatgcact caagttttac gactacgtca atgaacgcgg cgggcgtgtt acacttttcg
      181 aaatccctca acccaaatct gattggaaga catcactgga agtttttaac caggttttag
      241 aacatgaaca gcacgttaca tcattgattc acaagctcta cgaaacagct ctttctgaaa
      301 aagattacgc gtcccaggtg atgttacagt ggttcataga cgaacaagtt gaggaagaaa
      361 agaacgccac cgagattgta gaacaactta agctcatcga tgtccatgga acagccgtcc
      421 tgatgctgga tcatcgtctc agtaagcgcg gcaaagaata aaccaaaccg tatcaatgag
      481 tatgtaagcg gcttttttct tcaaatttca acaaaaccat atgaacctaa gggggttgta
      541 tctattagtt tattagtagg taacaagcga ccacaacttt ggaggtgaat tgtgggtaat
      601 attcgaattg aaaaactagc acccctctca ggtgtcatat cccttttgct gttttgcatt
      661 ggggcggcac tgctcggtat ctacgaatat ctaccaatag ccgataaact taaggccatc
      721 ctcgacacca acgcttccag catttttacc ggtggctacc ttggctcaat ctcagcattc
      781 ttcatgattt ggttcgcagg cagcgtatac agcgcattac gcgaacaaga aggtggtacg
      841 ggacgcttgt ctatggtggc atttggaggt ggtgttgcat ctggtgttgc ccttgcatct
      901 ggattctccg caatcttgac ctccggggcg agagctggtg ccgcaggtgg catcaccgca
      961 atcgaagcag ttaccctgta tgatctttat agccaagtct taggaggcat gtttgctatc
     1021 actatggcag tctttatcgg agccacaggg accgtctcac ttcgcacagc catgttcccc
     1081 agttggtttg gctgggtgag cgtattgatt gccatcggtc ttctcacccc ttttgcatat
     1141 gccgttcttg ctcttgtact gatttggttg atcatcgtta gcttctggtt gtatcggcgc
     1201 ggtctatcaa caacataaag attcgcccaa gctgcgaagc gagttttgcc cgaaactcaa
     1261 tttctatacc accaaaggac aatccttcgc accacttaaa cttgagcgaa gccccgtcag
     1321 cttgatgctg gattagacag atctttccgt ttgagtttaa ttcctgcaat cacccgtctt
     1381 gcaacatctg tgtaaactat caaggctacg gtggaccaat tatttggacc actaattttt
     1441 ggcaatctat tttctctctt tcaagataca acttcaatgg tttacataaa cgacaaattc
     1501 cgcccctcca tacgcataac ccatagatct ctggaatatc tgcttccctt caaacaaatc
     1561 atcataaata gaagaacaaa taattaccca cacttttctt ggactttctt tcaaagagct
     1621 tattattttc tcgatgattt tttccat
//
LOCUS       DBNHOH010000580        10131 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_354764, whole genome shotgun sequence.
ACCESSION   DBNHOH010000580 DBNHOH010000000
VERSION     DBNHOH010000580.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10131)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10131)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10131
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_354764"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..1486
                     /locus_tag="AC16V7_10060"
     CDS             233..1486
                     /locus_tag="AC16V7_10060"
                     /inference="COORDINATES: protein motif:HMM:NF024241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6257504.1"
                     /translation="MNNISQQAKRISVTLFSAQSLASAGFITAATLNSIVGAKLGGSA
                     SWAGVPSAVYLLGGALAAYGWGYLMDVWGRRGGLVAGLFIGAVGAGLTFQAIAMHSLP
                     IFLVGMALMGIANAAVTLGRFAAAEVHPPQSRGRAISNVVLGGAVGAILGPLLVGPMG
                     ELAKSQGFDELSGGYVASGVLFLFACLTVYGGLRPDPRDLGREIAQLFPETAKLSGVT
                     RSIGQLLRQPAVKIAMIAMVLGQMVMVMVMVITAQHMRSHQHSLNAVSLVISSHTFGM
                     FGFSVLSGRLADRWGRGMVIIIGSTTLLLSCLAATISPEVLPLAVSLFLLGLGWNFCF
                     VGGSTLLADQLSPSERAPTQGFNDLFVGLASASASFSSGLIYDQSGYTIMALAGAAFS
                     LAPLLATSIWLRGLNRSPMPALPRS"
     gene            complement(1545..2843)
                     /locus_tag="AC16V7_10065"
     CDS             complement(1545..2843)
                     /locus_tag="AC16V7_10065"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIR6257505.1"
                     /translation="MKRKHFIIVLAMLVLVACQSSVGTLESTASPAPPVGESPVKTPT
                     SLPTATPVVVTGTVRIWHSWEDPFVPALLRAIDGFNELYPSIYFDVLYVPADDLPIAF
                     EQAVQEGHAPSLLIGQAEWGPDLYDKGLVADLSDLISPDLLDTLNPAALGTGRLAVGL
                     ISVPVDIRGVVLYRNRSIIPIAPSTFEELASLAKEATRGQTLGSVLDRSFFFSGAHLI
                     GLGGSLMTEEGKPGFNNERGLDWVGLLQAYDQAGAAEFFTDNDLNMFKEGRVGFIIDG
                     TWNRTSLAETIGADNLAIDPWPIHAGGSLSGFVQAESMFLTPQALDEVHAVSLKFLQF
                     FLSPEAQSSIAEVGLIPAINGSPVNLAANQVVISDPLISQAMRALTDGVSYPANPEMG
                     IYSSQLDISLKSVFDDGVPAQQALQSAEEAINAALVTPAP"
     gene            2924..3838
                     /locus_tag="AC16V7_10070"
     CDS             2924..3838
                     /locus_tag="AC16V7_10070"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012904.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR6257506.1"
                     /translation="MTTAHRLSGVYAAAITPLKPDFSPDPNSLPKLLDFLAQRGCHGV
                     LLFGTTGEGPSIAPKERLLMLRAALEYRQTHPTFRLLFGTGTPSFEETVELTKTAFDL
                     GVDGVVVIPPYYFRKISDEGLLIWYSQLIQRAVPSGGAFLGYHIPPVTGIDLSFDLLR
                     RLRDAFPDRFAGIKDSSADLEHARNLGKHFGNELVVLNGTDRLFSHALQAGASGCITA
                     LANLFSPALRRVWDSHLRGEEDEQAQARLNSARALSESYPPAPPLLKMLLAHWYNFPL
                     WPVCPPLLPMPPSTAEKARVDFEPLRYD"
     gene            3831..6239
                     /locus_tag="AC16V7_10075"
     CDS             3831..6239
                     /locus_tag="AC16V7_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6257507.1"
                     /translation="MTESQDNTAQIRFLLTNLGIIFIFILVLVVILATYPALFAPTPT
                     PTSTRVLSPPQTATSTPTITSTPTATRTRRPTFTATITLTPSITPTPTLTPTPTGLPT
                     LTPASPLSGDNIYRLTAWTPELADYVVSLIGNYPNTLLRQQRGENDENYYTAFYFATI
                     AQFEAILRFPTAPQAEGWRWGLAYNLALTGNPLAGEQYAELIARALNRGDSTPQDISA
                     WFEKQDPRLTLNTTPLQPPPPYLSGQLLQIEGNGSTFILLLETPSAFQTQVLASNFDF
                     AHSPEYKALARDLTGDNIQEIVIYPTTPAQSRQLSLPRIFSLAQSPPQEPAFNSVSAN
                     FNIGMDYTVDWIAAQDEQGGSDLQVRSTLFPACPMELKRTYRWDGDWFQPVKTEYLVT
                     PKPTTLSFCRLLVDHAISVWGPDSAIQIMQTILPDWPPATKEDGTPFPLDARDEWRFR
                     IGIYHALLGEQDTATRAFQEIINSPSVPNSTWIARAQSWLNSYKDSQHLYRACVATEY
                     CKPAQALTYLIGSLRRSDYPSAAAFLGEAGVRFTSSGYFDFDGDDSTETWFTVRHRPG
                     EKLEFWLLAPYSQGVKAILVDLIDTNVPSLSYLDQESIPPVVLFHDNKAFRLHRLVGT
                     LEPYLTFQELPKFYRDRFKEPLKIAIQDLFNGEDPATIQKTLLDLEVYPGLLCRGTWS
                     CDEYYYMLGLASELAGDQKQAINTYVQLWSDYSKSPFTSMARLKLSGRAIGPAATQSP
                     TPQPFATGTSTPTITGTPPTRTATPTATRTATPGTPYPIDTTSPEPYPLDTPVSTTGY
                     TPYP"
     gene            6303..7070
                     /locus_tag="AC16V7_10080"
     CDS             6303..7070
                     /locus_tag="AC16V7_10080"
                     /EC_number="2.3.1.-"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF046470.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HIR6257508.1"
                     /translation="MAADEAILEAVTRGDAPPTLRLYAWQPPCLSLGHAQPWSDVDWK
                     RLTAAGWDLVRRPTGGRAILHTDELTYAVIGPQEEPRLKGGVLESYRRLSQALLEALN
                     LLSLQAQAKPIAASDENGEAKGPVCFEVPSNYEITVNGKKLLGSAQARKQGGILQHGT
                     LPLWGDLTRITQALHYESESQRERSAARLQERATTVEEILGHRPTWEAATRAFISAFK
                     QELALELEPAELSAAEQGRAMELVSEKYGNPIWTERI"
     gene            complement(7134..7832)
                     /locus_tag="AC16V7_10085"
     CDS             complement(7134..7832)
                     /locus_tag="AC16V7_10085"
                     /inference="COORDINATES: protein motif:HMM:NF025380.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HIR6257509.1"
                     /translation="MANDNNLRGWVLVIGVLLILGLAAFGVVSVVQQSANQAKQAIQP
                     VNELTSNVATQVAQFLNPTPTVLPDPVTIIQGVRSLSRLETIQYSVEKVITAETNQGP
                     FGFLFGDRLILVAHGIVIAGVDLEKLTPENMQLRGGVLYVQLPEAEIFVATLDNDKSY
                     IYNRDTGLLTKGNINLETEARRAAEDEIELATLEDGILEQANQNAENYLYRLLRDLGY
                     PEVIFIRITPEPQD"
     gene            7906..8190
                     /locus_tag="AC16V7_10090"
     CDS             7906..8190
                     /locus_tag="AC16V7_10090"
                     /inference="COORDINATES: protein motif:HMM:NF016351.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HIR6257510.1"
                     /translation="MTLGIITLHLQIPGCTSLKEKRRRLKPLLARLHRQFNISVAEMD
                     QHDAWQESLIACAVVSNDIGFTTRSLQAIPDWVEVHWPDVVVLDNQIEVF"
     gene            complement(8331..8933)
                     /locus_tag="AC16V7_10095"
     CDS             complement(8331..8933)
                     /locus_tag="AC16V7_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6257511.1"
                     /translation="MSLPALIILVGLAYIVLFGGLSLLRREGLSSRFAIEALVITFMV
                     GGLTALTGIQIHPIMFLIVLYLITMRVRLLVDIANFFAQRSQFERANLFYNLAQRLWP
                     DQTGHLVIQVNQGTALLQNDALDEAIAVFTDVLKNANQGYLGVKYEAATHYNLGIAYR
                     RKDMEPQARLEFTAVLDTWPASQYAQRAEIALSKDRKKSN"
     gene            9225..>10131
                     /locus_tag="AC16V7_10100"
     CDS             9225..>10131
                     /locus_tag="AC16V7_10100"
                     /inference="COORDINATES: protein motif:HMM:NF014598.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HIR6257512.1"
                     /translation="MNAVLTLVLGLVGIGIGYGFYARTINAGVVKPDDKKATPARMFM
                     DGVDFTPANRNVLFGYQFKSIAALGPIVGPIVAIQWGWLPALLWVIFGTFFIGWVQDY
                     SAIIMGVREEGQSFGALSYRFVSPRARTILLMFIYFYLLLIMGAFGKIVGYDLMTNPA
                     VPLGVLLVIGVGLLAGQMTYKWKMDIILTSVVTVVLAFLGIWLGTLPGVKSLFLAING
                     GEKSPVVFGSVTQAQFIWSLVVLVICYFGAVLPIWRWAQPINYVAFWIVFLGIVAGTI
                     GLLVWHPSFPSDFPAFTNWAGNLCQT"
BASE COUNT         2446 a         2774 c         2470 g         2441 t
ORIGIN      
        1 ggcaaggcga tggctttgac cggctatgat gtgctcacga aaaaagattt tctggaagct
       61 gcccgcgccg agttcaccgc cagcctgggg tatccaccag gagggaaaag gagcacccca
      121 aatccaatag agtaattata gtatctttat ctatttagca aaaaaagtta taattacttc
      181 aagaaatcca atattttcaa ttgcctcctt atcctcacaa tactcaatac caatgaacaa
      241 catctctcag caagctaaac gtataagcgt gacgctattt tctgcccaaa gcctggcatc
      301 ggctggtttc atcaccgcag ccacactcaa ctcgattgtg ggcgccaaat tgggcgggag
      361 tgcatcctgg gcgggtgtcc cttcggcggt ttatctcctg ggaggtgcgc tggcagccta
      421 cggctggggt tatctgatgg atgtctgggg caggcggggt gggctggttg ctggcctgtt
      481 cattggcgca gtgggtgctg ggctgacttt tcaagccatc gctatgcact cattgccaat
      541 tttcttggtc ggcatggcac tcatgggtat cgcaaatgct gctgtaactc tgggacgttt
      601 tgccgctgct gaagtacacc ctccccaatc tcgtggtcgg gcaatctcga atgttgtatt
      661 gggcggggca gtaggagcaa tactgggccc gcttttggta ggcccaatgg gtgaactcgc
      721 gaaatcgcag gggtttgatg agctatcagg tggttatgta gccagcggtg tgctgttcct
      781 ctttgcctgc ttgacggttt acggcgggct acgacctgat cctcgtgatc taggacgtga
      841 aattgcccaa ctcttccctg agacggcaaa actcagcggg gtaacacggt cgattggcca
      901 gctcctgcgc cagccggcag tgaaaatcgc catgattgct atggtgctag gacaaatggt
      961 gatggtaatg gtgatggtca tcaccgccca gcatatgcgc tcccaccagc atagccttaa
     1021 tgctgtatct ctggtcattt catcccatac atttgggatg tttggtttct cagttttatc
     1081 tggtcgactg gcagatcgct ggggtcgcgg catggtaatt attatcggtt caaccacgct
     1141 cttactatcc tgcctggcag ccacaatatc tcccgaagtc ctgccattgg cggtatcgct
     1201 ttttcttttg ggtcttggct ggaacttttg ttttgtaggc ggctccacct tgctggccga
     1261 tcagctctcg ccgtctgagc gtgcacctac ccagggtttc aatgacctgt ttgttggcct
     1321 ggcgtctgca tccgccagct tcagcagtgg gttgatatac gaccaatcag gctacaccat
     1381 tatggcattg gctggagcag cattctcgct cgccccttta ctggctactt caatctggct
     1441 aagaggactg aatcgcagcc caatgccagc gttaccaaga tcataacggg cagcacgtta
     1501 taacccccaa tcacagatga catcctggat ccagcgggaa taggttacgg tgcgggtgtc
     1561 actaaagcag cattgatcgc ttcttcggcg ctctgcaacg cctgctgtgc tggcacacca
     1621 tcgtcaaaga cagacttcaa ggaaatatcc aactggctgg agtaaatgcc catctccgga
     1681 ttcgctggat agctgacgcc atccgtcagg gcgcgcatgg cttgggagat gagtgggtct
     1741 gagatgacga cctgattagc agccaggttg accggagagc cgttgatggc tgggatcaag
     1801 ccaacctctg caatagagga ttgcgcttcg ggtgaaagaa aaaactgtaa aaatttcaat
     1861 gatacggcat ggacttcatc caatgcttga ggggtaagaa acatgctctc tgcctgtaca
     1921 aacccagaga gagagccacc tgcatgaata ggccagggat cgattgccag gttatctgcg
     1981 ccaattgtct ccgccaggga agtccgattc cacgttccat cgatgataaa gcctacgcgt
     2041 ccctctttga acatgttgag atcattatcc gtgaaaaact cggctgcccc cgcctggtcg
     2101 taagcttgca gcaagcccac ccagtcaaga cctctttcat tattaaaacc tggcttcccc
     2161 tcttctgtca tcaaactgcc accaagacca atcaggtggg cgccggagaa aaagaagcta
     2221 cgatcaagca cagaacccaa agtctgcccg cgggtggctt ccttagccag gcttgctagc
     2281 tcttcgaatg tggatggagc tattgggata atacttctat tccggtacag gaccacaccg
     2341 cgaatatcta caggaacgct gatcagacca actgcaaggc gcccggtgcc cagcgcagca
     2401 ggattaagtg tatccagcag gtctggtgaa atcagatcag acaggtctgc caccaaccct
     2461 ttatcgtata agtctggccc ccactctgcc tgcccaataa gcaaactcgg ggcatgccct
     2521 tcctgaacag cctgttcgaa ggcgataggc aggtcatcag cgggcacata gagcacatca
     2581 aaataaatgc tgggatatag ctcattaaac ccatcgattg cccgcaacaa tgccggcacg
     2641 aatggatctt cccacgaatg ccagattcgc accgtcccag tcaccacaac cggtgtggct
     2701 gtaggtaatg atgtcggcgt cttaacgggg gattcgccaa caggcggtgc gggggaggca
     2761 gttgattcga gagtgcccac actgctctgg caagcgacca aaacaagcat ggccaggaca
     2821 ataataaaat gtttcctttt catcctgatc cttttagaga gggtattgat tcgcctgaag
     2881 gataattgta ccagtttcga gcaataaaca gtataatcac cccatgacca cggctcaccg
     2941 gctgtccggc gtatatgccg cagcgattac cccgctcaag ccagattttt ctcccgatcc
     3001 taactccttg cccaagctgc tcgattttct cgcacagcgc ggttgccatg gggtcttgtt
     3061 gtttggcacc acaggtgaag gtccatcgat tgcacccaag gagcgcctgc tcatgctgcg
     3121 ggcagccctg gagtatcgcc agactcaccc cactttccgc ttgctcttcg gtacgggaac
     3181 acctagcttc gaagagaccg tggagctcac caagaccgcc tttgacctcg gtgtcgacgg
     3241 tgtagtagtc ataccgccct attattttcg aaaaatcagc gacgaggggc tgctcatctg
     3301 gtacagccag cttatccaac gagccgtgcc gtccggcggt gcattcctag gctaccacat
     3361 cccgcctgtc acaggcattg atctttcctt cgacctgctg cgacggttaa gagacgcatt
     3421 tccagatcgt ttcgctggga tcaaggattc ctccgccgac cttgagcacg cccgcaatct
     3481 gggaaaacac tttggaaatg aattggtggt gctgaatggc accgaccgcc tgttcagcca
     3541 tgccttacaa gctggggctt ctggctgtat cactgccctg gcgaatttat tctctcctgc
     3601 gctgcgccgg gtatgggatt cacacctcag gggtgaggaa gatgaacaag cgcaagctcg
     3661 cctgaactct gcccgcgccc tttcagagag ttatccacct gcgcctccct tgctcaagat
     3721 gctactagcg cactggtaca atttccctct atggcctgta tgcccgccac tgctgccgat
     3781 gcccccaagt acagccgaaa aagccagggt ggatttcgaa cctctacgct atgactgaat
     3841 cgcaagacaa cactgcccaa attcgctttt tactcaccaa ccttgggata atatttattt
     3901 ttatcctggt gctggtggtt attttggcaa cttacccggc tctcttcgca cccacgccta
     3961 cccctaccag cacgcgcgtc ttgtctcccc cacagaccgc aacctccacg ccgacaatca
     4021 catccactcc cacagccact cgcactcgcc ggcccacctt cacagcaact attacactca
     4081 caccttctat aacgcccacc ccaaccctga cacctacccc aaccggcttg cccacactta
     4141 cacctgcaag cccgttatcc ggcgataata tttaccgcct cacagcttgg acacctgaat
     4201 tagctgacta cgtggtgtca ttgatcggga actatcccaa caccttgctc cggcagcaac
     4261 gcggcgagaa tgatgagaac tattacaccg cattttattt cgccacaatt gcccaatttg
     4321 aagcgatatt gcgctttccc acagcccccc aggctgaagg ctggcgctgg ggtttagcct
     4381 acaacctggc gctgactggc aaccctctag cgggggaaca gtatgccgag cttatcgccc
     4441 gggcacttaa tcgcggcgat tccacccccc aagacatctc cgcttggttt gaaaagcaag
     4501 atccgcgatt gaccctaaat accacacccc tccaaccccc tcctccctac ctcagcggtc
     4561 aactgctgca gattgaagga aatggcagca ccttcatctt gttacttgaa acaccatctg
     4621 ccttccagac ccaggtgctt gcaagcaact ttgatttcgc gcacagccct gaatataagg
     4681 cgttggctag agatctgaca ggggataaca tccaggaaat cgtcatctat cccacaaccc
     4741 cggcgcaatc tcgccaatta tctttgccgc gcattttcag cttggcgcaa tcccctccgc
     4801 aagagccggc gtttaactca gtatctgcga acttcaatat tggcatggac tataccgtgg
     4861 actggatagc ggcacaggat gagcagggtg gctctgatct gcaggttcgc agcacccttt
     4921 tcccggcctg ccccatggag ctcaaacgca cttaccgttg ggatggagat tggttccaac
     4981 cagttaagac tgaatatctg gtgacaccta aaccgaccac gctgagcttc tgtcgcttgc
     5041 tcgtggatca cgccatcagc gtatggggac ctgattcggc tatacaaatt atgcaaacca
     5101 tcttgccaga ctggccaccc gccacgaaag aggatggaac acctttcccc ctggatgcgc
     5161 gcgacgagtg gcgcttccgc atcggtatct accatgcctt gctgggcgag caggatacag
     5221 ccacgcgcgc ctttcaagag atcatcaatt ctccttctgt accgaacagt acctggattg
     5281 cccgggcaca gagctggctg aacagttaca aagactccca gcacctttat cgcgcttgtg
     5341 tcgccacgga atactgcaaa ccagctcaag ccctcaccta cctaatagga agcctgcgac
     5401 gcagtgatta cccctccgcc gctgccttcc ttggggaggc cggcgtgcgc ttcacatcct
     5461 ctggttactt cgactttgat ggcgacgact cgacggaaac ctggtttacc gtgcggcatc
     5521 gccccggtga aaaactggaa ttttggctcc ttgcgcctta ttcccagggg gtcaaagcca
     5581 tattagtcga cttgatcgac accaacgttc cgtcgctttc ctatctggat caggagagca
     5641 tcccgcctgt tgttttattc cacgacaaca aagcattcag actgcatcgg ctggttggta
     5701 ctttagagcc atacctcact tttcaagagc tacccaagtt ttaccgtgac cgcttcaagg
     5761 aaccgctgaa aatcgctatc caggatctgt tcaatggtga agatcccgcc acaatccaaa
     5821 agaccttgct agacctagag gtatatccgg gtctgttgtg ccgcggcact tggagttgcg
     5881 acgaatacta ctacatgctt ggcctggcgt ccgaactggc aggggaccag aagcaagcca
     5941 taaataccta tgtgcaattg tggtcagatt actctaaaag ccccttcact agcatggctc
     6001 gcttgaaact aagcggaaga gctattggac ctgctgccac gcaatcacca accccacaac
     6061 cttttgcgac aggcacctca acgccgacca tcacgggaac gccaccaacc cggactgcca
     6121 ctcccacagc tacccgaact gccacccccg gcacgccata tcccatcgat actacctcac
     6181 ctgaaccata tcctcttgac actcccgtat ccacaacagg ttacacgcct tatccttagc
     6241 ttatgtacgc tcctgcaacc tggcgactaa ttatctcacc accttctcac ggcgcgtgga
     6301 atatggcggc ggatgaggct atccttgaag cggttacccg aggagacgcc ccacctacac
     6361 tgcgcctgta tgcctggcag ccaccttgcc tatcgctggg tcatgcccag ccgtggtccg
     6421 atgtcgactg gaaacgattg acagccgccg gatgggatct tgtacgccgc cccacgggcg
     6481 gacgggctat tttgcacacc gatgagctga cgtatgcagt catcggtcca caggaagaac
     6541 cgcgtttaaa ggggggtgtg ctggagagct accggcgcct gtcccaggcg cttctggaag
     6601 ccctcaacct gctgagcctc caagcacagg ccaaaccaat tgctgcgtca gacgagaacg
     6661 gagaggccaa aggcccggtg tgcttcgaag tcccatcaaa ttatgaaata actgtcaatg
     6721 ggaagaagct gttgggtagc gctcaagcgc gtaagcaggg aggtatacta caacacggga
     6781 ctttaccgtt atggggcgac ctgacacgca tcacccaggc tctacattat gaaagcgaga
     6841 gtcagagaga acgatccgca gcgcgcctac aagagcgcgc tacaaccgtt gaggaaattc
     6901 tggggcatcg tcctacctgg gaggcagcta ctcgggcatt catctcagct tttaagcagg
     6961 agctagcgct ggagctggag ccagctgaac tctccgccgc agagcaaggc cgcgccatgg
     7021 agttggtcag cgaaaagtat ggaaatccta tctggactga gcggatctag ctgccaggaa
     7081 ttgttctctt atagcctttg aagatcgcaa gcgggtgaga tcgccccaag gtatcaatct
     7141 tgaggttcag gggttattcg aatgaagatc acttcagggt agccaagatc gcgcaataag
     7201 cggtaaagat agttttcagc attctggttt gcttgctcta aaataccatc ttccagggtt
     7261 gctaattcga tctcatcttc cgcggctcgg cgagcttcgg tctctagatt aatattcccc
     7321 ttggtcagca ggccggtatc tctattgtat atataagact tatcgttatc caaggtggcg
     7381 acaaaaatct ccgcctcagg gagctgaaca tacaaaacac cgccacgcaa ttgcatgttt
     7441 tcgggtgtga gtttttccaa atccacccca gcaatgacaa tgccatgcgc caccaggatc
     7501 aggcggtcgc cgaagaggaa accgaatgga ccctggttgg tttcagcagt gatcaccttc
     7561 tccacgctgt actggatcgt ctccaggcgg gataacgagc gcaccccctg gataatggtc
     7621 acgggatcag gcaacacggt gggggtaggg tttagaaatt gggccacttg agtggcgaca
     7681 ttgctggtca attcgtttac cggctggata gcctgcttgg cctggttagc actctgttgc
     7741 acaacagata ccacgccaaa tgccgctagc cccaggatca acaacacgcc aatgaccaga
     7801 acccaaccac gaagattatt gtcatttgcc ataaaaataa ttatactgta agtacctacg
     7861 cagttgaaga aaagacccta tgcgtatctg ttgtcgatat aggctatgac cctaggtatc
     7921 atcacccttc acttacaaat accaggatgc acctcgctga aagagaaacg tcggcggttg
     7981 aaaccccttc tggcgcgctt gcatcgccaa ttcaacatct cagtagccga gatggatcaa
     8041 cacgacgcct ggcaagagtc actcatcgcc tgcgcagtgg tgagtaacga tattggattc
     8101 accacgcgca gcctgcaagc catacccgac tgggttgagg tgcactggcc agacgtggtt
     8161 gtgctcgaca accagattga agtattctag agactgctga aaataacaat tgggggattt
     8221 tatctaatgt ttattaaagt agctcgattt ttttcgttgt atatatatat atcaagccat
     8281 aatttagaaa ccgagctaag gtgcgggtgt tttaagaatt aagaaactcg ttaattactc
     8341 tttttacgat ctttcgataa cgctatttct gcgcgttgag catattgaga agcaggccac
     8401 gtatccaaaa cagcagtaaa ctccagccta gcctggggtt ccatatcctt acgccgataa
     8461 gctatgccaa ggttgtagtg ggtagcagct tcatatttaa ctccaaggta accctgatta
     8521 gcatttttta agacatctgt aaaaacagca atagcctcgt cgagggcatc attttgtaaa
     8581 agcgctgttc cttggttcac ttgtatcacc aaatgaccgg tttgatcggg ccataacctt
     8641 tgagcaagat tatagaagag atttgccctc tcaaattgag agcgctgtgc aaaaaagtta
     8701 gcgatgtcaa ccaatagcct gactcgcatg gttatcaggt aaagtacgat cagaaacatg
     8761 attggatgga tctgtattcc ggttaatgca gtcaaaccac ccaccataaa ggtgatcacc
     8821 aatgcttcaa tagcaaatct acttgataac ccttcccgac gaaggagtga taatcctcca
     8881 aagaggacaa tataggcaag acctacaagg atgatcagcg ctggcaaact cacataaacc
     8941 tccgaaaaga tagctgaata ccagttagta tacaacaaat gagaggatta tggtgttctt
     9001 tgtaacacct cacatgtaac aatctacatt tgacttatga gaacaaattc ctctacaatc
     9061 gagtatgcgc ccaaaacatg gagaagattt gttcggatga tcttcaaata tatccgtaga
     9121 gggggatcag aggattgttc ttgtcaaagg aggtgccatc gataattctg ctctaataac
     9181 aacaatttag atcaaatatt ttgtagaaat gaggagaatt tgcgatgaat gcagtcttaa
     9241 cccttgtttt aggactggtg ggcatcggca ttggctatgg cttttatgcc agaaccatca
     9301 atgccggcgt cgtcaaaccg gatgacaaga aagctacgcc agctaggatg tttatggacg
     9361 gcgttgactt cacccccgct aaccgaaatg tattgttcgg ataccaattc aaatccattg
     9421 cagcgttagg gccgattgta gggccgattg tagccatcca atggggctgg ttacctgctc
     9481 tattatgggt gatcttcggc acattcttca tcggctgggt gcaagactat tcggcaatca
     9541 tcatgggcgt gcgagaagag ggtcagagct ttggcgcgct gagctatcgc tttgtttccc
     9601 ccagagctcg aacgattttg ttgatgttca tttacttcta cttgttgctc atcatgggcg
     9661 catttggaaa aatcgtcggc tatgacctga tgaccaatcc ggcagttcca ttgggtgtgc
     9721 tattggtgat tggtgtaggt ttgctggcag gacagatgac gtacaagtgg aagatggata
     9781 tcattctaac cagtgtagtc actgtcgtat tagccttctt aggtatttgg ctcggcacat
     9841 tacctggtgt gaaatcgctc ttcctggcaa tcaatggagg cgaaaaatca cccgtggtct
     9901 ttggctcagt cacgcaagcc cagttcattt ggagcctggt agttctggta atctgctact
     9961 ttggtgccgt actgccaatc tggcgctggg cgcaaccgat caactacgtg gctttctgga
    10021 tcgtcttcct gggtattgtt gctggcacca tcggcttgct ggtttggcat ccatccttcc
    10081 catccgattt cccagcattt acgaactggg caggcaatct atgccaaaca g
//
LOCUS       DBNHOH010000581         3538 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1316772, whole genome shotgun sequence.
ACCESSION   DBNHOH010000581 DBNHOH010000000
VERSION     DBNHOH010000581.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1316772"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="AC16V7_10105"
     CDS             complement(<1..538)
                     /locus_tag="AC16V7_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR6257513.1"
                     /translation="MKKILSSEITPEQVYLNRRQFIKLGALTAGALALAACGEASDEK
                     KVSDTKAEYAGATVNPQSSELKDELDDTANTYEEITNYNNYYEFSMDKEAVAPLSKDL
                     KTRPWSIEVGGLVHNPKTYAIEDILSKFPAEEHIYRLRCVEAWSMVIPWLGFPLKKLL
                     DEVEPTSQAKYVRFQTLLD"
     gene            complement(828..1763)
                     /locus_tag="AC16V7_10110"
     CDS             complement(828..1763)
                     /locus_tag="AC16V7_10110"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR6257514.1"
                     /translation="MYILGENIHIISEKVKEALANKDQKFFQEQAVKQVEAGAQALDL
                     NLGPRKKDWEEVFPWIVKTVEAVVDVPLCFDSTNILGIEAGLKHVSKAQPIINSTSAE
                     PERLEKVPLLAKKYNTKLIALTIGSGGIPVAADERVSIALETLIPRMLEIDFPISDLI
                     IDPLVLTVSGCQEYCPQLIEAVRTLEYAWDPPPTISVGLSNVSNAVPHERRSLINRVY
                     CAMLMGVGLQMMIANPLEEKLKDTIRIIEERDESTPVSRLYLKIADRIQAMEEPSIDD
                     VDMDDPEQEAIWKTVQVLLNKVIYADAYLGQLEVM"
     gene            complement(1844..>3538)
                     /locus_tag="AC16V7_10115"
     CDS             complement(1844..>3538)
                     /locus_tag="AC16V7_10115"
                     /inference="COORDINATES: protein motif:HMM:NF025925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HIR6257515.1"
                     /translation="CQTVVEGDVSISVPPQEKIERRLTTDRTVAEIEVPEGYDFARSQ
                     TVRRLTLMLTPPSMDDQTDDLSRLRIGMRQQADIGDIDISLPLLRKIGAVLREDDWRV
                     TAILESESWDKPARSARLIDLRPGHELLNEPVWGAAIDIGTTTITLWVVDLVTGKVHA
                     QVAEYNGQISRGEDVISRIVFSAKNGGSDQLRSLVLETINALLDRACKRVKITPDQVV
                     KASIAGNSTMMHLLLGIPATSIRLAPFITAVNHLPLLSAHEVGLAINPEGVVDCLPGI
                     ASYVGADISAGVFSSGMSNTENLTLFLDVGTNGETVLGSREWLVTCACSAGPAFEGAG
                     VIHGMRATKGAIEEVWVNSDTFEPTFRVIGGVKPRGLCGSGLISLLSEMFITGLVDKG
                     GNINAHLGIPRVREGEHGLEYVIVWGQDSAIGEDIVITHVDIDNLLRAKAAIYAGFTI
                     LADSVGIPLEAVEQVLIAGSFGKYINVEKAVQIGLLPDMPWERFQFLGNTAIRGAYYA
                     LLDKEAREEIRAVASRMTYIELSADNTFYDAFMSALFLPHTDISRFPSVEGVMSKT"
BASE COUNT          937 a          924 c          744 g          933 t
ORIGIN      
        1 ggtcaaggag ggtctggaat cgcacgtact tcgcttgcga ggtgggctcc acttcatcca
       61 ataatttctt taaagggaag cccaaccagg gtatgaccat agaccaggct tcaacacaac
      121 gcaggcggta aatgtgttcc tcggccggaa atttactcaa aatatcttcg atagcatacg
      181 ttttcggatt gtgaaccagc ccacccacct caattgacca cgggcgagtt tttagatctt
      241 tgctcaatgg agcaacagct tccttgtcca tggaaaattc ataataatta ttatagttag
      301 taatttcctc gtaagtgtta gctgtgtcat caagctcatc tttcaattca ctcgattggg
      361 ggttgacagt cgccccggca tactcagcct tggtatcaga aacctttttc tcatctgatg
      421 cctccccaca cgccgccaaa gcaagcgcgc cggctgtgag cgcccctagc ttgataaact
      481 ggcgtctatt tagataaacc tgttcaggtg tgatctcaga ggacagaatt tttttcattg
      541 tatactccta acccaattca tatgtgctaa tagtgtacct gttaataatt aactatttat
      601 tacaaataag ataataatat tctaatataa tttggggtta tttatggctg aactattcag
      661 cctaccacaa ttttctatat gactatgcgc agttacgagg acttaaacaa ccctagactg
      721 ggtgatgtac ctcagtttgc tccattaaaa acatgaggag cttgggcagg cttcgcccgc
      781 caaaactcct cggtcatttc cactcctgcg taatataaat ggtgaattta catcacttct
      841 aactgcccaa gataggcatc cgcatagatt accttattca agagaacctg tacggttttc
      901 caaattgctt cctgctcagg atcatccatg tctacatcat caatggaagg ctcttccatg
      961 gcttgtatgc gatcagcaat cttcaagtac aggcgactaa caggtgtcga ttcatctcgt
     1021 tcttcgataa tgcggatagt gtccttcaat ttttcctcta aaggattagc tatcatcatc
     1081 tgcaagccaa cccccatcaa catcgcgcag taaacgcggt tgatcaatga ccttcgttcg
     1141 tgaggtacgg cattggaaac attggagagg cctaccgaaa tggtcggcgg cggatcccag
     1201 gcatactcca atgtccgcac cgcctcaatc aactgaggac agtattcctg gcatcccgat
     1261 acagtcagga ccaagggatc gattatcaga tcggaaatag gaaaatcgat ctcaagcatt
     1321 cgcggaatca atgtctcgag agcaatagat acacgctcat cagcagctac cgggattccg
     1381 cccgagccaa tcgtcagggc aatcaacttg gtgttatatt tttttgccag caaaggcact
     1441 ttttctagcc tttcaggctc cgcagacgtt gaattaatga ttggctgagc tttactgaca
     1501 tgcttcaacc cagcttcgat tcccaggatg ttggtgctgt caaaacaaag cggcacatcc
     1561 acaacagctt cgacggtttt tactatccag ggaaatactt cctcccaatc cttcttgcgt
     1621 ggacccagat tcaaatccag agcttgtgca ccggcttcaa cctgttttac agcctgctcc
     1681 tggaaaaact tttgatcctt gtttgccagg gcttctttga ccttttcaga gatgatgtgg
     1741 atattttcgc ctaaaatata catggtattt cgctccctcg attttttgca ggtctaatcg
     1801 atctgagcgc aagattatta tcctgcgcat tacaggttct ccctcaggtt ttactcatca
     1861 cgccttcaac ggatggaaag cgggaaatat ccgtatgagg taaaaacaat gccgacatga
     1921 acgcatcata aaaagtgtta tcagcagata gctcaatata cgtcatcctg gatgctactg
     1981 cccgaatttc ttcacgcgcc tctttgtcca gcagcgcgta ataagcaccc cgaattgcag
     2041 tattgcctaa aaactggaaa cgctcccaag gcatatcggg aaggaggcca atctgaacag
     2101 ctttttcgac attgatatat ttcccaaacg agccggcaat gagcacttgt tcaactgcct
     2161 caagtgggat gcctacgcta tctgccagga ttgtgaaccc agcgtatata gccgccttag
     2221 cacgcagtaa attatcaata tctacgtggg taataacgat atcctctcca atggcgctat
     2281 cttgacccca aacaatgaca tattcgagtc catgctcgcc ttcacgcaca cgtggtatgc
     2341 ctaaatgagc attgatgtta ccgcccttgt ccactaaccc ggtgatgaac atctccgaaa
     2401 gcagcgagat taaaccactg ccacatagtc cgcgtggctt cacgccaccg attacacgaa
     2461 acgttggctc gaaagtatca ctgttgaccc atacttcttc aatagcccct ttggtagctc
     2521 gcataccatg tatgacgcca gcgccctcaa aagctggtcc agccgaacag gcacatgtca
     2581 caagccactc tcttgaaccg agcacagtct cgccattggt gcctacatct agaaataggg
     2641 tcagattctc ggtattgctc atgcccgatg agaatacacc cgcgctaata tcagcaccaa
     2701 catagcttgc gatgccagga aggcagtcaa ctactccttc aggattaatt gccaagccaa
     2761 cctcatgcgc agagagtaaa ggcaaatgat ttacagctgt aataaatggc gccaaacgga
     2821 tgctggttgc cggaatacct aaaagcaagt gcatcatggt gctgttaccg gcaatacttg
     2881 ccttcaccac ttgatcggga gttattttga ctcgcttgca ggccctgtcc aacagtgcat
     2941 tgatggtttc aagcaccagt gagcgcaact gatcacttcc gccgtttttg gccgaaaaga
     3001 caatacgaga aatcacatct tcgccgcgcg aaatttggcc attgtactct gcaacctgtg
     3061 cgtgcacttt tcctgtgact agatccacaa cccataaagt gatcgtggtc gttccaatgt
     3121 caatggcagc cccccacaca ggttcattca ggagttcatg tccaggtcgg aggtcgatca
     3181 aacgcgccga gcgagccggt ttatcccaag attcggattc caaaatcgcc gtgacccgcc
     3241 aatcgtcttc acgtaatact gccccgatct tgcgcaatag aggtaatgag atatcaatat
     3301 cgccaatatc ggcttgttgt ctcatcccaa ttctcagacg actcaaatca tcagtctggt
     3361 catccatgct gggtggagtg agcatcagtg ttaacctgcg gacggtctgt gatcgggcaa
     3421 aatcataacc ctcagggacc tcaatctctg caaccgtgcg atcagtagtg aggcgccgct
     3481 cgattttttc ttgtggcggc acactaatag atacgtcacc ctcaacaacc gtctgaca
//
LOCUS       DBNHOH010000582         1002 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1060917, whole genome shotgun sequence.
ACCESSION   DBNHOH010000582 DBNHOH010000000
VERSION     DBNHOH010000582.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1060917"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="AC16V7_10120"
     CDS             <1..340
                     /locus_tag="AC16V7_10120"
                     /inference="COORDINATES: protein motif:HMM:NF012976.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dioxygenase family protein"
                     /protein_id="HIR6257516.1"
                     /translation="YKPDAPLRDKVGEGYVLQGMVLSSAGCVPIPGAQLEFWLAGSDG
                     EYSDDYRATLLADDKGAYRFESNLPPPYSGRPPHIHIRISAEGYQTLVTQHYPNEGET
                     SANMDIVLIP"
     gene            complement(388..>1002)
                     /locus_tag="AC16V7_10125"
     CDS             complement(388..>1002)
                     /locus_tag="AC16V7_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257517.1"
                     /translation="SPRFGMILFPSLWITAGVGAHLLVADLNQQWLRKASYLGFLCVL
                     ILASAGNFRLFMSRLFAEHENANPGVNQAYNFVSTVLDIPTKNGSAIVMIGRTDQWNG
                     PALRFHLVSQCLLVERGCEIDVRDTWELRKGWPTQELPEEVQRQRLDTALEQADYLVN
                     FFDHPESPAGWELISERQFTFERLHKKPAIIWVSIYQHGSSASP"
BASE COUNT          277 a          255 c          237 g          233 t
ORIGIN      
        1 ctataagcca gacgcgccat tgcgcgacaa agtgggagaa ggctatgtac tgcaaggcat
       61 ggtgctctct tcggcgggtt gtgttcctat acctggggca cagctagagt tctggctggc
      121 tgggtccgat ggcgaataca gcgacgacta tcgcgccaca ttgctggccg atgacaaagg
      181 cgcctatcgt tttgaaagca acctgccccc accctactcg gggaggccgc cgcacattca
      241 tatccgcatc agtgcggaag gataccaaac gctcgtcacg cagcattacc ccaatgaggg
      301 ggagactagc gcaaacatgg atatagtgct tataccctaa tctttttagc gcactttata
      361 cacacgggca tactcgattc cgttgaatca aggagaagca gatgatccat gttggtagat
      421 tgagacccat atgatagctg gttttttatg caatcgctca aatgtgaatt ggcgctcaga
      481 gattaactcc cagccagccg gactctcagg gtgatcgaaa aaatttacta aataatctgc
      541 ctgttcgaga gccgtatcga gtcgttgcct ttgaacctct tcagggagtt cttgcgttgg
      601 ccaacccttg cgcaactccc aagtatcgcg cacgtcaatt tcacaacccc tttcaacaag
      661 caaacactgg gataccaagt gaaagcgcaa agccggtcca ttccactgat ctgttcgtcc
      721 gatcatgaca atagcagaac catttttagt tgggatatca agaactgtag agacaaaatt
      781 gtaggcttga ttaacaccgg ggttagcatt ctcatgctca gcaaatagcc tggacatgaa
      841 taacctgaag ttgcctgcgc tggctaaaat taatacacat agaaatccca aataactggc
      901 tttacgcaac cattgttgat tgaggtcagc caccagcaaa tgagcaccca cgcctgctgt
      961 aatccaaagc gaaggaaaaa gtatcatgcc gaagcgcgga ct
//
LOCUS       DBNHOH010000583         4051 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_354964, whole genome shotgun sequence.
ACCESSION   DBNHOH010000583 DBNHOH010000000
VERSION     DBNHOH010000583.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_354964"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="AC16V7_10130"
     CDS             complement(<1..193)
                     /locus_tag="AC16V7_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HIR6257518.1"
                     /translation="MTEIFIIGQRYEVPEGLTILRAFEWAGFRLTRGVGCRGGFCGAC
                     GTVYRLNSDYHLRYALACQT"
     gene            complement(190..639)
                     /locus_tag="AC16V7_10135"
     CDS             complement(190..639)
                     /locus_tag="AC16V7_10135"
                     /inference="COORDINATES: protein motif:HMM:NF024581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR6257519.1"
                     /translation="MPLRQQTDPQRIGSILHHVFSLPYPPVARCRLLSSGFGYGYMLK
                     TTDDLSGSKACLGCGCCMDACPVLARDSSRRIRSEARTSMALETLVGDDCDRCGNCAL
                     ACPQVDPTIKHFLVQTHLAEGMAELLVKATSDEMFVLDVMLSDWSSP"
     gene            complement(649..1677)
                     /locus_tag="AC16V7_10140"
     CDS             complement(649..1677)
                     /locus_tag="AC16V7_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257520.1"
                     /translation="MKTSFLLGTNGDALGVVRQFLQELWSYADLDGMLVPIYHADGIN
                     VSPYFVEDPSHLMLADPLAPMVSASSAKMVPQITDFNPKARLGFVWHSCEARAWQWLI
                     EQKNIAPGNWLIIGVDCLSSFSSKDFGWRVERAGNVERLTRETMRFARMGGIAPYRYR
                     DACQMCTSPVVQDADLNIELLGLPVKSVLIITAKDREIVNKLRLRELTDGEAPPSLVS
                     RREATLGVLSGRRRQAAERMEQGLSEDMPAEVGSLLQYFANCAPCQECLEACPIYMGE
                     FSPATNGGAKAISAATRWLESCISCGMCDDACPKQMPLTALFRHIHHALQDDINLTPA
                     SNLAGIAS"
     gene            complement(1674..3413)
                     /locus_tag="AC16V7_10145"
     CDS             complement(1674..3413)
                     /locus_tag="AC16V7_10145"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HIR6257521.1"
                     /translation="MFTPKVGVYICHCGINIAGTVDVGAVADYAASLPGVAISRHYTY
                     MCSDPGQALIKQDITELELNRIVVASCSPLMHEPTFRAVIAEAGLNPYCLEMANIREQ
                     CSWVHPKSWQTTQKAMQLVASAVVKAAHLQPLEVRHAPVIPATLVIGGGVAGMQAALD
                     IAEAGFGVTLVERSSELGGNVAGLHRTFPTLNPAAELLRPLMQRVQHHPRIQVLTAAE
                     VCDVSGYVGKFQVQVRQADQLVNVSVGTILVATGFEAFDAHRKPELGYGEYPQVMTTL
                     DFDRLARTDLLVNGKQPERVVFIQCVGSRERSSGDQNGRNPYCSRVCCMTVARQARLV
                     RERLPDAKVTVFYMDVRAYGKGFEEFYDEVRQHGVIYRRGNPSEIVQRGACVVVRAED
                     TLLGKPVEVEADLVVLAIGMTPVSDSAHLASLLKLARSADGYFLEAHPKLRPVDTALA
                     GVFLAGCCQGPKDISESITHARAAAAAAMIPMMRGQMPVEAATAFVDEEMCSGCNQCA
                     QSCSFSALQLHPVRGVMKVNPVLCQGCSACATACPSGAINVHRFTTDQVLGQIEAFSI
                     VEPELVMSNGGVR"
     gene            complement(3434..>4051)
                     /locus_tag="AC16V7_10150"
     CDS             complement(3434..>4051)
                     /locus_tag="AC16V7_10150"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR6257522.1"
                     /translation="EESCCGSEVRRLGEAGLFEMMVEENQELFKSLGIHKMVTTSPHC
                     FNVFKNDYPKNGMQVEHYTQLLSGLVDQDSGAAGGHLSFTGSVDAKVTYHDPCYLGKH
                     NHIFEEPRKVLLSIPGLQLVEMDRSREKSLCCEGGGGRMWLEGTNPGVRLAQSRIKEA
                     LETGAEILATACPFCLLTLEEAVKHLNAEDKIRVMDIAEIAAQAL"
BASE COUNT          988 a         1204 c         1000 g          859 t
ORIGIN      
        1 ctgtttggca ggctaaggcg taccgcaggt ggtagtcgct gttcaagcgg taaacagtac
       61 cgcaagcccc gcaaaatccc ccccggcagc cgacaccacg cgtaaggcga aatccagccc
      121 actcgaaagc cctcagaata gtcaaaccct cggggacttc ataacgctga cctataatga
      181 aaatttctgt catggcgaac tccaatcact taacatgaca tccagcacga acatctcgtc
      241 tgatgtggcc ttaaccagta gttcggccat cccttcggct aaatgtgttt gcaccagaaa
      301 atgcttgatg gtaggatcaa cctgtgggca agccagtgca cagttcccgc aacgatcgca
      361 gtcgtcgccc actaaggtct ccaatgccat ggaggtgcgc gcttcactgc gtattctacg
      421 gctggagtct cgagccagca caggacaggc atccatacag cagccacaac ctaggcaagc
      481 ttttgaaccg ctcaggtcat cggtcgtctt cagcatgtag ccatacccaa agcctgagct
      541 cagcaaccgg caacgcgcga caggtgggta cggcaaagag aaaacatgat gtagtataga
      601 tccgatccgc tgtggatcag tttgttggcg aagcggcatg gcaacctcct aggaagcaat
      661 tcctgccagg ttagaggctg gagtcaggtt aatatcatcc tgcaaggcgt gatggatatg
      721 gcggaacagt gcggtcagtg gcatttgctt ggggcaagca tcgtcacaca taccgcagga
      781 gatacaagac tccaaccagc gtgtagcggc agagatcgcc ttagcaccac catttgtggc
      841 tggtgaaaac tcacccatat agatcgggca ggcttctagg cattcctgac acggagcgca
      901 attagcgaag tactgaagca agctgcccac ctcggcgggc atatcctctg acaggccttg
      961 ttccatacgc tccgccgctt gtctcctgcg tcccgacagc acccccaacg tggcttcacg
     1021 ccgcgatacc agcgatgggg gggcctctcc atcggtgagt tcccgaagac ggagcttgtt
     1081 gacgatctct cggtctttcg ctgtaatgat caatacactt tttacaggca gtcctaataa
     1141 ctcaatgttc agatcagcgt cctgtacaac cggtgaagtg cacatctggc aggcatcacg
     1201 gtatcggtag ggcgcgatgc ctcccatgcg tgcgaagcgc attgtctcac gagtgaggcg
     1261 ttctacattg cccgctctct ccacacgcca cccaaagtct tttgaggaga aactagacag
     1321 gcaatccaca ccaataatta accaattccc aggggcgata tttttctgct caatcagcca
     1381 ttgccaggca cgcgcctcgc aagagtgcca aacaaagcca agccgcgctt ttggattgaa
     1441 atccgtgatt tgcggtacca ttttggccga acttgcagaa accatcggcg cgagaggatc
     1501 agctagcatt aagtgagatg ggtcttccac aaagtaggga gatacattga tcccatcggc
     1561 atgatagatg ggcaccaaca ttccatccaa atccgcatac gaccagagct cctgcaagaa
     1621 ctgcctcacg acccccaatg catcaccatt ggtccctaat agaaagctcg ttttcatctt
     1681 acacctccat tactcattac taattcaggc tctacaatcg agaatgcttc aatctgcccc
     1741 aaaacctggt cagtggtaaa gcggtgaacg ttgatcgctc cgctcggaca ggccgtggcg
     1801 caagcgctac acccctggca caacaccgga ttcaccttca ttacgccccg aacgggatgc
     1861 aattgcagcg ctgaaaagct gcaagattgt gcgcactggt tacaacctga acacatctct
     1921 tcatccacaa aggcagtcgc tgcttccacg ggcatctggc cgcgcatcat ggggatcatc
     1981 gctgcggcag ccgccgcccg ggcgtgagtg atcgattcgg agatatcttt gggaccctgg
     2041 cagcacccgg ccaggaaaac gcccgccaaa gccgtgtcca cgggacgcag cttcgggtgc
     2101 gcttccagga agtagccatc tgcagaacga gccagcttga gcaagctggc gagatgagcg
     2161 ctatctgaaa ccggcgtcat gccgatcgcc agtaccacca ggtcagcttc tacttcgacc
     2221 ggtttgccga gcaaagtatc ctcggcgcgc accaccacac acgcgcccct ttgcacaatc
     2281 tctgatggat ttccacgccg gtagatgacc ccatgctggc gaacttcatc atagaactcc
     2341 tcaaaaccct ttccataggc gcgcacatcc atgtaaaaca cggtcacttt ggcatctggc
     2401 agacgctccc ggaccaggcg ggcctggcgg gcaaccgtca tacaacaaac ccgtgagcaa
     2461 tacgggttgc gcccattctg atctcccgaa gagcgctcgc gcgagccaac acactggatg
     2521 aaaacaaccc tttcaggctg tttcccattg acaagtaaat ccgttcgagc caggcgatca
     2581 aaatccaacg tggtcatcac ctggggatat tccccatatc ccaattcagg ttttctgtgc
     2641 gcgtcgaagg cttcaaagcc cgtggcgacc aaaatagtcc ccacggatac attcactaac
     2701 tggtcagcct gacggacctg cacctgaaat ttaccaacat aaccgctgac atcacaaacc
     2761 tctgccgccg tcagcacttg aattcgaggg tgatgctgca cccgctgcat cagaggtctg
     2821 agaagctctg cagcagggtt aagggttgga aatgtgcgat gaagaccagc cacattccca
     2881 cccagctcag agctgcgctc aaccagagta acgccaaagc cagcctcagc gatatctaag
     2941 gcggcctgca tgcctgccac ccccccgcct atgaccaggg ttgcgggtat gaccggggcg
     3001 tggcgcactt ccagcggctg cagatgggct gccttgacca ccgcagaagc taccagctgc
     3061 atggccttct gcgtggtttg ccaactcttg ggatgcaccc aagagcactg ctcgcggata
     3121 tttgccatct ccaggcaata tggattcagt ccagcctcag cgatcacagc gcggaaggta
     3181 ggctcatgca tcagcggtga acaggaagca accacaatgc ggttgagctc cagttcggta
     3241 atatcttgct tgatcaacgc ttgccccggg tctgagcaca tataggtgta atggcgagag
     3301 atcgctacgc ccggcaacga ggcagcgtaa tcagctaccg cacccacatc tactgtccca
     3361 gcgatgttga tgccgcaatg gcagatgtac acgcctactt ttggtgtaaa cataatgaaa
     3421 taattctccg tccttagagg gcttgagcag cgatctccgc aatatccatg acgcggatct
     3481 tgtcctcagc attgaggtgt ttgacggcct cctccagggt gagcaagcag aaggggcaag
     3541 cggttgccag aatctccgcg ccggtttcca gggcttcctt tatacgtgat tgcgccagtc
     3601 gaaccccggg attagtgccc tccaaccaca tgcgtccgcc gccaccctcg caacacagac
     3661 ttttctcccg agaacgatcc atctcaacca gctgcaagcc tggaatgctt agtaaaacct
     3721 tgcgcggttc ttcgaatata tggttgtgtt tgccaaggta acatggatcg tgataagtga
     3781 ccttggcatc aacactgcct gtaaaggaga gatgacctcc cgctgcaccg ctatcctgat
     3841 ctaccaaccc ggacagcaat tgggtgtaat gttctacttg cattccattc ttgggataat
     3901 cattcttaaa aacattgaag cagtgcggcg aggtggtaac cattttgtgt atccccaagc
     3961 ttttaaagag ctcttggttt tcttctacca tcatctcgaa aagccccgcc tcacccaaac
     4021 gacgcacttc actgccacag cagctttcct c
//
LOCUS       DBNHOH010000584         1731 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_227119, whole genome shotgun sequence.
ACCESSION   DBNHOH010000584 DBNHOH010000000
VERSION     DBNHOH010000584.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_227119"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1731)
                     /locus_tag="AC16V7_10155"
     CDS             complement(<1..>1731)
                     /locus_tag="AC16V7_10155"
                     /EC_number="1.2.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF013481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HIR6257523.1"
                     /translation="FLRHYLRAAGKPRQINPLSPENPLIFGAGMLTGTIAPNAARFNV
                     SARSPESGILGDANCGGFFAAAMRKAGFDRLVILGKAERPAYILLEDGVVHILDASDL
                     WGTRVVEAQEGLRSKHGPGTISAVIGPSGENQVRMAAVMTGIKNAAGRGGMGAVMGSK
                     NLKAIAARGGTAIEVAQKEAIRSTRLEQQKYLKDSKIVQILGRVGTPLLYEVSNRIGA
                     IRTRNSQDNYFEDSLNSEEIEKYSDKMLACTSCVVHCRHRNTLGGEGPEYSTIGLLGA
                     NLGIAPTDQVIMLNNQVNELGLDASSVGTIIGWAMELYQRGLIDEGMTEGPLTWGDYE
                     VARELIEKIAYRQGFGNILAESSQALRYFPPEAKDYLIAVKGLPQSDPHDVRYFKGFA
                     LGIAVASRGADHLRNRPTLEVFKLPDEVRAKIYGRANDPDPTGYKDKGLIVAWSDDIY
                     AVTDSLGICKFVTHGFNSPHLLGYEHFIELVSAATGLEYTPETLRQVGRRVLDTERLI
                     NADFDLTRDDDTLPKRYFDDPMPARKSKGHHVDRQQFQAMLDDYYTECGWDNQGRVPV
                     DRQQAIDEMI"
BASE COUNT          347 a          494 c          480 g          410 t
ORIGIN      
        1 caatcatttc gtcgattgct tgttgcctat ccacgggaac ccgcccctgg ttatcccagc
       61 cgcactctgt gtagtagtcg tccagcatgg cctgaaattg ctgacggtcc acgtggtgtc
      121 ctttggactt gcgagcgggc atagggtcat cgaaatagcg cttgggcagg gtgtcatcgt
      181 ctcgggttaa atcaaagtct gcgttgataa ggcgctctgt atccagcacc ctgcgcccaa
      241 cctggcgtaa tgtttcgggg gtatattcca gaccagtagc agcagaaacc aattcgataa
      301 aatgctcata gcccagcagg tgcgggctgt taaacccgtg ggtgacgaat ttgcaaatgc
      361 caaggctatc ggtgacggcg tagatgtcgt cgctccaggc aacgataagc cccttgtctt
      421 tgtaccctgt ggggtcggga tcgttggcgc ggccgtaaat cttggcccgc acttcatcgg
      481 gaagcttgaa gacttccaat gtggggcgat ttcttagatg atctgcgcct cggctggcaa
      541 cggcaattcc caatgcaaat cctttgaaat agcgcacgtc atgcgggtcc gattggggca
      601 gacctttgac tgcgatcaaa taatccttgg cctcgggggg gaaatagcgc agcgcctggc
      661 tgctttcagc caggatattg ccgaaacctt gccggtatgc gatcttctca atcagctcac
      721 gtgcgacctc gtaatcgccc cacgtcaatg gtccttcagt catcccctcg tcgatcaagc
      781 cgcgctggta aagttccatc gcccagccaa tgatcgtgcc gaccgaagaa gcatccaaac
      841 caagctcgtt aacctggttg ttaagcatga tgacctgatc ggtgggggca atacccaggt
      901 tggcacccag caaaccgatg gttgaatatt cggggccttc gccgccaagt gtgttgcggt
      961 gtcggcagtg gaccacacaa gacgtgcaag ccagcatttt gtcggagtac ttttcgatct
     1021 cctctgaatt caggctgtct tcaaagtaat tatcctgact gttgcgcgtg cggattgcgc
     1081 ctatgcggtt gctgacctcg tagaggaggg gggtgcctac tcgccccaag atttgcacga
     1141 tcttggaatc cttgaggtat ttttgttgct ccaggcgagt cgatctgatg gcttctttct
     1201 gtgccacctc gatcgcagtg ccgccacggg ctgcgatagc tttcagattc ttgctgccca
     1261 tcacggcgcc catcccgcct cgcccagcgg cgttcttgat tccggtcatc acagcagcca
     1321 tgcgcacctg gttttcacca gacggtccga tgacggcaga gatcgttccg ggtccgtgct
     1381 tggacctcaa gccttcttgc gcttcgacta cccgcgtgcc ccataggtcg ctggcatcca
     1441 gaatgtgcac aaccccatct tccaggagaa tgtaagcagg gcgctcggct ttccctaaga
     1501 tcaccaggcg gtcaaatccg gctttgcgca ttgccgccgc aaaaaaaccg ccgcaattag
     1561 catctcccaa tatgccgctt tccggtgagc gggcagatac gttgaagcgc gcggcattcg
     1621 gggcgatcgt gccagtcagc atgcctgcac cgaagataag cgggttctct ggactgagcg
     1681 ggtttatctg gcgcggcttg cctgccgccc gcaagtagtg gcgcagaaat g
//
LOCUS       DBNHOH010000585         7297 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_355112, whole genome shotgun sequence.
ACCESSION   DBNHOH010000585 DBNHOH010000000
VERSION     DBNHOH010000585.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7297)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7297)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7297
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_355112"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..476
                     /gene="def"
                     /locus_tag="AC16V7_10160"
     CDS             3..476
                     /gene="def"
                     /locus_tag="AC16V7_10160"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR6257524.1"
                     /translation="MRRKARKVTSFGPQLQSRIDDMVETMREAPGVGLAAPQVDVSWR
                     VIVVEFGDEEDEEAPKKLYTVVNPEISRASQEMSVGTEGCLSIPGFVGEVERPDAITI
                     KGLNRYGKPFRINADGWLARIFQHEVDHLEGVLFIDRAEKVWKVEEADAEVAAAD"
     gene            476..1711
                     /gene="recF"
                     /locus_tag="AC16V7_10165"
     CDS             476..1711
                     /gene="recF"
                     /locus_tag="AC16V7_10165"
                     /inference="COORDINATES: protein motif:HMM:NF014516.7"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIR6257525.1"
                     /translation="MFLKRLSLTNFRNYARLDVDVPTGSVLLVGDNAQGKTSLLEAVY
                     FLATFVSFQADSDRQLINFIAGREPLAVARIVADFQRQELDHTSRDHRLEVRIIQEAN
                     GINGGSRVRKEVLLDGVERKISEAVGVFNAVLFQPQTLRIIEGAPEDRRRYLNLTLAQ
                     AVPRYTAALTDYNRAITQRNALLKLLNERGGDPNQLTYWDELLASSGASLIYARIQAI
                     KGLERLAARIHSQLTQGAEVLRLSYLPAYDPLPKPERQYALPIDAPLDRSGIPLEKIQ
                     QGFFESLERLHSEEIARGVTTIGPHRDELRFLANGIDLGVYGSRGQSRTAILALKLAE
                     VAWLKEETGQWPVLLLDEVLAELDVQRRTDLLERLADIEQALLTTADLEAFSKDFIRQ
                     ATLWRIYAGQLEELKHVPE"
     gene            1698..2198
                     /locus_tag="AC16V7_10170"
     CDS             1698..2198
                     /locus_tag="AC16V7_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR6257526.1"
                     /translation="MSLNNPTMGERVGWIFQINASNGGVPKLSRKLGEVTFNGLNGDN
                     QANPDVHGGPERALCLYSLERILALQAEGHPIFPGSAGENLTLAGLDWSLVNPGTRLQ
                     LGADVLVQVTRYTSPCNTISGSFMKNDYSRISQKVFPGWSRVYAQVIQPGTIQVSDAV
                     RISTMD"
     gene            complement(2226..2936)
                     /gene="rsmG"
                     /locus_tag="AC16V7_10175"
     CDS             complement(2226..2936)
                     /gene="rsmG"
                     /locus_tag="AC16V7_10175"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR6257527.1"
                     /translation="MRELARLVHSFLGIRLSASQLAALTSYEAELVEWNSRFNLTAID
                     TPENIRIKHFLDSLTCLLVMKDRPMERIIDIGTGAGFPGLPIKIVYPSVALTLVDSVG
                     KKVDFCRHIAQKLGLENVDIIQRRAEELGQLPEHREQYDWAVARAVAVLPVLVEYMLP
                     LVRVEGGMIAMKGESAPVEAHSADNALRLLGGRLKHLVPVTLPGVADERYLVVVDKVV
                     ATPLQYPRRVGIPAKKPL"
     gene            2867..3880
                     /locus_tag="AC16V7_10180"
     CDS             2867..3880
                     /locus_tag="AC16V7_10180"
                     /inference="COORDINATES: protein motif:HMM:NF024919.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HIR6257528.1"
                     /translation="MQPAGKQTGVSPKTSAPGAPVPSYSRTHYTWGYHGFGKKIRQLL
                     ALVFLGILLPITRPLSAKADHLPERAYIRGVIGHAQSYPLSCESRSAADWAAFWDVNI
                     RERKFLTKLPRSENPDEGFVGNPSDAWGNIPPKSYGVHAEPVAALLREHGLQAEARRG
                     MQWDELRAEIAAGRPVIVWVIGQIWQGTPIKYSAPDGHKTTVARFEHTMIVIGYGPSV
                     VHLVDAYSGKTVSHPLRRFLASWKTLGNMAVVGQANPEEPSKPEQGNSSQVFLPVIFS
                     GTSETSQSVSPTPSPKTYVVKRGDYLVGVARQFSVNWRKLANLNGIKYPYTIYPGQVL
                     KIP"
     gene            complement(3981..4856)
                     /locus_tag="AC16V7_10185"
     CDS             complement(3981..4856)
                     /locus_tag="AC16V7_10185"
                     /inference="COORDINATES: protein motif:HMM:NF025616.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HIR6257529.1"
                     /translation="MAPEAGAPPFEELAAREMAEPASAVFMDDAQGEIMPAVGQTQTE
                     RLVIKNADLTIVVDDPVKSMDNITYLAEEMGGFVVSANLREMTLDSGAKSPRASITIR
                     VPAADFNDALTRIKEETEQLPLNENISSQDVTSEYTDLQSRLRNLEATEDQLTEIMGS
                     ANKTEDVLSVYNRLVQVREQIEVIKGQIQYYEQSAAMSLISVELIANEAVQPLTIGGW
                     QPVGTAKDAVQTLINTLKVLGNLVIWIILYVLPVLVVLFVIFVLPLRLVWQAWRRRKA
                     KGKESLPPAPPSQST"
     gene            complement(4999..5727)
                     /locus_tag="AC16V7_10190"
     CDS             complement(4999..5727)
                     /locus_tag="AC16V7_10190"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR6257530.1"
                     /translation="MRILIISDIHSNLTALEAVLADAGQVDAVWCLGDLVGYGPDPNE
                     CISCVRELPNLACIIGNHDAASLNQIDSATFNPEARDAVRWTRDTLSDASVAFLQGLP
                     ERVEMDNVTLVHGSPRHPVWEYLLDTKNATQSFAYFESAYCFVGHTHLPSIYLLGTDN
                     HSARLMIPEANSRLSLSPRAILNPGSVGQPRDRDPRAAYAIYFPEEDIWEHRRATYQV
                     KSVQERMVAAGLPDRHIQRRAAGW"
     gene            complement(5814..6623)
                     /locus_tag="AC16V7_10195"
     CDS             complement(5814..6623)
                     /locus_tag="AC16V7_10195"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6257531.1"
                     /translation="MLYSIWRGRGKMVGLTYNIGVKSATAEKLIALNHQFYQTLALQF
                     SATRMRLQPGVRRSLETLPSQVNILDLGCGNGELARELAKRDHQGFYMGLDFSSEMLA
                     EAQRSRLETLKAFFIQVDLSSPDWFTTIQRLQPGILEHPLDVILAFAVLHHLPGDDLR
                     KLVLRDVRKNLAPHGLFIHSEWQFLNSPRLRQRIQPWESIGLNESDIDEGDYLLDWRS
                     GGYGLRYVHHFTEQELMQLASETGFTVQETFYSDGESGKLGLYQIWQPDLI"
     gene            6610..>7297
                     /locus_tag="AC16V7_10200"
     CDS             6610..>7297
                     /locus_tag="AC16V7_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ELP3 family radical SAM protein"
                     /protein_id="HIR6257532.1"
                     /translation="MEYNNFRFRPHTPETLEIARLVLNDVHSGRKVLDAIRRHPLPGG
                     GYLSKSMLISAYKQLAESGIYEIDPSFLARIRMKPVRTLSGVTTVTVLTKPYPCPGKC
                     IFCPTDVRMPKSYLPDEPGAMRALQHDFDPYGQVVGRIQALQAVGHPTDKIELLVLGG
                     TWNSYRRDYQEWFIRRCFDALNGFASDTLIDAHQYNETAAHRAVGLVVETRPDHVNPQ
                     EIAWLRYLGVT"
BASE COUNT         1769 a         2029 c         1799 g         1700 t
ORIGIN      
        1 ccttgcgacg caaagctcgc aaggtaacga gttttggacc acagctgcag tcgcgtattg
       61 atgacatggt agagaccatg cgcgaagccc cgggtgtggg cttggccgcg ccgcaggtcg
      121 acgtctcatg gcgggtcatt gttgtggaat tcggcgatga ggaggatgaa gaagctccga
      181 aaaagctcta caccgtggtc aacccagaaa tatctcgcgc atcccaggag atgtctgttg
      241 gtacggaagg ttgtctctcc atccctggtt ttgtcggtga ggtggaacgc cccgacgcca
      301 tcacgattaa gggattaaat cgttatggca agccatttcg gataaatgct gatggctggc
      361 ttgcccgtat tttccaacat gaggtcgacc acttagaggg tgtcctgttt attgatcggg
      421 cggaaaaagt ctggaaggtc gaagaagccg acgctgaagt agccgccgca gattaatgtt
      481 tctcaagcgc ctttcgctaa cgaattttcg caactacgct cgcctggacg tagacgtgcc
      541 caccggttcg gtgcttttgg tgggcgataa cgcccagggg aagaccagcc tcctagaggc
      601 tgtatatttt ttagcaacct ttgtttcatt ccaagccgat agcgaccgcc aattgatcaa
      661 tttcatcgcc gggcgcgaac cactagccgt ggcgcgtatc gtggcagatt ttcagcgcca
      721 agaactagac catacttcgc gtgaccaccg tctggaggtc cgcattatcc aagaagcaaa
      781 tggaattaat ggtggtagcc gcgtgcggaa agaggtgctc ctggatggtg tcgaacgaaa
      841 aattagcgag gcagtggggg tattcaatgc cgtgctcttt cagccgcaaa cccttcgtat
      901 catcgaaggt gcccctgaag accgtcgtcg ctatctcaac ctgaccctgg ctcaggctgt
      961 tccccgctac acagctgcac tgacagatta taatcgcgcc atcacccagc gcaacgcctt
     1021 gctaaaactg ctcaatgagc gtggcggcga ccccaaccag ctaacctatt gggatgaatt
     1081 attggcttct tccggagcca gcttgatcta tgcccgcatc caggctatca aagggctgga
     1141 gcgcctggcc gcacgcatcc acagccagct gacacaggga gccgaagtat taaggctatc
     1201 ctacctgcca gcctacgatc cactgccaaa acctgagcgc caatacgcat tacccatcga
     1261 tgcgccactc gatcgctccg gcatcccatt ggaaaaaatc cagcaaggtt tttttgagag
     1321 tctggagcgc ttacattctg aggagatcgc ccgcggtgtc accacaattg gtccccaccg
     1381 cgacgaatta cgcttcttag caaatggaat tgatttgggg gtatacggtt cacgcggcca
     1441 gtcgcgcact gccatcctgg ccctcaaact agccgaggtg gcctggctaa aagaagagac
     1501 cggccaatgg ccagtgctct tgttagacga agtcctggct gagctcgatg tacaacgccg
     1561 cactgaccta ttagagcgct tggcagatat tgaacaggct ttgcttacca ctgctgatct
     1621 ggaagctttc tctaaggatt tcatccgtca ggccaccctc tggcgtatct acgccggcca
     1681 gctcgaggag ttgaagcatg tccctgaata acccgaccat gggagagcgc gttggttgga
     1741 tttttcaaat taacgcctca aatggcggtg tgcccaagtt aagccgaaag ctcggcgaag
     1801 tcaccttcaa cggtctaaat ggggataatc aggccaaccc ggatgttcat ggtggaccag
     1861 aacgggcgct ttgcctttac tctctggagc gtatccttgc cttgcaagct gaaggacatc
     1921 ccatattccc gggatcagct ggtgaaaacc tcactttagc tggtctggat tggtctctcg
     1981 tcaatccggg cacccgtctc caactgggag ccgatgtgct agtgcaggtg actcgctaca
     2041 catccccctg caataccatt tccggttcat ttatgaagaa cgactactct cgtatctctc
     2101 agaaagtctt tccaggctgg tcacgtgtct atgcgcaagt catccaaccg ggcacgattc
     2161 aggtgagtga tgcagtacgc atttctacaa tggactgata aagaaataag agaatcaatt
     2221 tttgcctata atggcttttt ggcgggtatg cccactcgtc tgggatattg cagtggtgtt
     2281 gctaccactt tgtccaccac caccagatat cgctcatccg cgaccccagg taaggtgaca
     2341 ggtaccagat gcttgagcct gccacccaac aggcgcaggg cattatccgc tgaatgtgct
     2401 tcaacgggag cactttcacc cttcatagcg atcatccccc cttctaccct cactagaggc
     2461 agcatatact ctaccagcac gggcagaact gccacagccc tggcaacagc ccaatcatat
     2521 tgctcacgat gctctggaag ctgacccagt tcctcggcac ggcgctgtat tatgtcgaca
     2581 ttttccaatc ccagtttctg agctatatgc cgacaaaaat cgaccttctt ccccaccgag
     2641 tcaaccaagg tcaatgccac agatggatag actatcttaa ttggcaatcc tggaaagcca
     2701 gctccagtcc ctatatcaat gatccgctcc atgggtcgat ctttcatcac caacaagcaa
     2761 gttaaggagt caagaaagtg cttgatgcgg atgttctctg gagtgtctat agcagtgagg
     2821 ttgaatcttg aattccattc caccaactct gcttcatagg aagtcagtgc agccagctgg
     2881 gaagcagaca ggcgtatccc caaaaacgag tgcaccaggc gcgccagttc cctcatactc
     2941 acgcacccat tatacctggg ggtatcatgg ctttggaaaa aaaatacgcc aactcctagc
     3001 gctcgtattc ctcggtatac ttcttcccat cacccgcccc ctatctgcta aagctgatca
     3061 ccttccagag cgcgcctaca tacgcggggt aatcggccac gctcagagct acccgctatc
     3121 gtgcgagtcg cgctcggcgg ccgattgggc tgcattttgg gacgtcaata tccgtgagcg
     3181 gaagtttctt accaaactgc cccgttcaga aaaccccgac gaaggctttg tgggaaatcc
     3241 aagtgatgcc tgggggaaca tcccgcccaa atcttatggc gtccatgccg agccagtagc
     3301 tgctctgttg cgtgagcatg gtcttcaggc agaagctcgg cgagggatgc aatgggacga
     3361 attgcgcgct gagatcgctg cggggcgccc ggtgatcgta tgggtcatcg gtcagatatg
     3421 gcagggcact ccgatcaaat attccgcacc cgatgggcac aagaccactg tcgcccgctt
     3481 tgagcacacc atgattgtca tcggttatgg tccatcggtt gtacatctgg tagatgctta
     3541 tagtggtaag acggtatctc acccgctgcg cagattccta gcctcctgga agacgctagg
     3601 aaatatggct gttgttgggc aagccaaccc cgaagagcca tcgaaacccg agcagggaaa
     3661 ttcgtcccag gtattcctac cagtaatatt ttcgggtaca tcggagacat cccagtccgt
     3721 ttctccaacc ccgagcccta aaacctacgt ggtcaaacgc ggcgattacc tggttggagt
     3781 ggcgcggcaa ttcagcgtca actggcgcaa acttgctaat ctgaatggca tcaaataccc
     3841 gtataccatt tatccaggtc aagttctaaa aattccatag atagcaaaag gtatctgcgg
     3901 cgggtgtaaa cccttgcaga taccttcttc gattatttat ctctctaccg tacagaggac
     3961 gttgaaatgg agaggtaata ttacgttgat tgtgaaggag gtgctggggg caagctctcc
     4021 ttgcctttcg ccttgcggcg ccgccaggct tgccaaacca atcgaagcgg cagcacaaaa
     4081 atcacaaata aaactaccaa cacaggcagc acgtacagga taatccaaat aaccaggttt
     4141 ccaagcacct tcagggtgtt gatcaatgtc tggacagcgt ctttggcggt gcccactggc
     4201 tgccatccac cgatggtgag cggctgaaca gcctcattgg caattaattc aacactgatg
     4261 agcgacatcg ccgctgattg ctcataatat tgtatctgcc cctttatcac ctcgatctgc
     4321 tcacgtacct gcaccaatct gttgtaaacc gatagcacat cttcagtctt gttcgctgaa
     4381 cccattatct ccgtaagctg gtcttcggtg gcttccaggt tgcgcaaacg cgactgcaaa
     4441 tcggtgtact cgctggttac atcttgactg ctgatatttt cattgagcgg gagttgctct
     4501 gtctcctcct tgatacgggt cagggcatcg ttgaaatccg cagccgggac tcgaatggtg
     4561 atagaagccc gcggagattt tgcgccgctg tccagcgtca tctcgcgcag attagccgaa
     4621 accacgaacc cccccatctc ctcagccagg taggtaatgt tgtccataga cttcacgggg
     4681 tcgtctacca caatggtaag atcggcattc tttatcacca agcgctcggt ctgtgtctga
     4741 cctacagcag gcatgatctc accctgtgca tcatccataa aaacagcgga ggctggctct
     4801 gccatctccc ttgcagccag ctcttcgaaa ggcggcgcgc cagcttcagg ggccaaggat
     4861 ctcgctgatg cgcaggctga aatccataat aagctcgttg ttaatatcaa agggagaatt
     4921 cttttgatca ttgccataac tcctttcacc ctatttctcc caagacgtcc cccgcaatca
     4981 aaatgttccc cacttccctt accatcccgc agcacggcgt tggatatggc gatctggcaa
     5041 accagctgcc accattcgtt cctgtacaga tttgacttgg taagtcgctc gacggtgttc
     5101 ccagatatcc tcctctggga aataaatcgc ataggcggcc ctcggatctc gatctcgcgg
     5161 ctggcccact gacccgggat ttaggatagc ccgaggcgaa agggacaaac gcgagttggc
     5221 ctctgggatc atcaacctgg cagaatggtt gtccgtgcca agtagatata tgctaggcag
     5281 gtgggtgtgc ccaacaaaac aatatgctga ttcgaagtaa gcaaaactct gagttgcatt
     5341 ttttgtatcc aataaatatt cccaaaccgg atgccgaggg ctgccatgca ccaaagtgac
     5401 attatccatc tcgacccgct caggtaagcc ctggaggaat gctacactag catcagatag
     5461 ggtatcccgg gtccatctga ctgcatctct ggcttctgga ttgaaagtgg ccgaatcgat
     5521 ctggttgagc gatgctgcat cgtgattgcc gatgatacat gccaggttgg gcagctcacg
     5581 cacacaggaa atacactcat ttgggtctgg accataaccc actaaatccc ccaggcacca
     5641 taccgcgtca acctgtccag catccgccag cacggcttcc aaagcagtca ggttggaatg
     5701 gatgtccgaa ataatcaaga tacgcatagg aacacctaat agtagcacag aataagcata
     5761 tcgttaagag atcagtcgac aaaccaccct gtaacaaatc aataacgatg cgctcaaatt
     5821 aaatcaggct gccagatttg gtataaaccc aacttgccgc tttcaccatc agaataaaaa
     5881 gtctcttgca cggtgaagcc cgtctcactt gccaattgca tcaactcctg ttctgtaaag
     5941 tgatgcacat agcgcaaacc atacccacca ctgcgccaat ctaacaggta atcaccttca
     6001 tctatatcac tttcgttgag cccgatgctc tcccacggct ggatgcgctg gcgtaatcga
     6061 gggctgttta aaaactgcca ttccgagtgg ataaacagcc catgcggggc aaggtttttg
     6121 cgcacatccc gtaaaaccaa tttgcgcaaa tcatccccag gcagatggtg caatacggca
     6181 aatgcgagga tgacatccag cggatgttcc aaaatgccag gttgtaaccg ctggatagtt
     6241 gtgaaccaat ctggggagga caggtctact tgaataaaaa aagccttcag ggtctcaaga
     6301 cgggaccttt gggcttctgc gagcatttca ctgctaaagt ccagccccat atagaacccc
     6361 tggtgatcgc gttttgccag ttcacgagct agttctccgt ttccacatcc aaggtccaag
     6421 atgttcacct ggcttggcaa ggtttccagg ctgcggcgca cgccgggctg caagcgcatg
     6481 cgggtggctg aaaactggag tgctaaggtc tggtaaaatt gatggttgag tgcaattaat
     6541 ttctctgcag tggcagactt cacaccgata ttataagtca gaccgaccat ttttccccgt
     6601 ccgcgccaga tggaatataa caattttcgc tttcgcccac atacaccaga aaccctcgaa
     6661 atagcccgtt tggtcctcaa cgatgtccat tccggtcgaa aggtgctgga cgccattcgg
     6721 cgacacccac tgccaggcgg tggttatctg agtaaatcca tgctgatctc agcctataaa
     6781 caactcgccg agagcggcat ctacgaaatt gacccatctt tcctggcgcg cattcgcatg
     6841 aaacctgtgc gcacactctc cggagttacc acggtaaccg tattaaccaa gccctaccca
     6901 tgccccggaa agtgtatctt ttgccccacc gatgtgcgca tgcccaagag ttacctacca
     6961 gacgagccag gtgctatgcg cgccctgcaa cacgattttg atccctatgg acaggtcgtg
     7021 gggcggatcc aggcgttgca ggccgtaggc caccccacgg ataaaatcga gttactggtc
     7081 ttgggtggga catggaactc ctaccgccgt gattaccagg aatggtttat ccgtcgatgc
     7141 ttcgatgcac tcaacggctt cgcctcagat actctgatag acgcgcacca gtataatgaa
     7201 acagccgctc atcgcgccgt gggattggtc gtggagacac gccccgatca tgtcaacccc
     7261 caggagatcg cctggttacg atatctgggc gtcacca
//
LOCUS       DBNHOH010000586         2443 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_804011, whole genome shotgun sequence.
ACCESSION   DBNHOH010000586 DBNHOH010000000
VERSION     DBNHOH010000586.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_804011"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="AC16V7_10205"
     CDS             complement(<1..418)
                     /locus_tag="AC16V7_10205"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR6257533.1"
                     /translation="MVNHFIDNEFVPGLEGGRFETLNPANNQPITEVASGSAPDADRA
                     VKAARAAFDQGPWPRLPAEERAKALRRIGDLIEKYADEIAEVEIADTGVPVSQIKKGA
                     IPRAAENFYFFAEMATQITGETYPVEGQFMNYTVRRP"
     gene            complement(441..1187)
                     /locus_tag="AC16V7_10210"
     CDS             complement(441..1187)
                     /locus_tag="AC16V7_10210"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR6257534.1"
                     /translation="MRLVRFSVAGQLHSGVVDPDGNLRSSQGERYNPDEVVWLPPVEP
                     SKVIGLALNYAEHASELQMAPPDEPALFFKPISSLVGHGAPIIYPSGVDYMHYEVELA
                     VVIGKRCRKVSRQDADQVIAGYTIANDVTVRDYVGNFYRPPVRAKGYDSFGPVGPTLV
                     TPNEIPDASKLELRAFVNGELRQKGNTASLIKTVPELIEFISQIMTLEEGDMIWTGTP
                     KGISHVYPGDVIRLEIDGLGVLENPVFAEE"
     gene            complement(1184..2059)
                     /locus_tag="AC16V7_10215"
     CDS             complement(1184..2059)
                     /locus_tag="AC16V7_10215"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6257535.1"
                     /translation="MTATDSSVEVNGIQLSYREWPGEKGPLICLPSLALHKGSFDAIA
                     SRLSPEYHLYALDLRGRGDSDKPAEGYGFAYHTRDVLTFAEKMGMQTFGIIGHSFGAT
                     VGVYLASIRPLSLRCIVMIDGGVDPKDEVMQAMRPLLGRLGTSYPSMDAYLNTMREIP
                     YYQPWNATLERVLREDVETLPDGTVRSKTSPQAIERDLDIHFYYSMCVHFPTMQCPSL
                     FIRPRQGLLGDKAHVLDEREAAAFVNWIPKCWRVDLPDVNHYTMVLSEEPVVVPPIRA
                     FLSTIFTEAGEESES"
     gene            complement(2067..>2443)
                     /locus_tag="AC16V7_10220"
     CDS             complement(2067..>2443)
                     /locus_tag="AC16V7_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR6257536.1"
                     /translation="AHITRLLGRLGRDFLVYSGIELLCFPILAIGGAGYVSATGNVLP
                     RQVAQLYDLFAAGKWRQAQDMHYYLMPLNDAMFIEINPVPAKTTLGMMGKISPEVRLP
                     LAPMSAENIGRLRGVLAQYHLL"
BASE COUNT          538 a          688 c          673 g          544 t
ORIGIN      
        1 ccgggcgacg gacagtgtag ttcatgaact ggccttccac cgggtaggtt tcgcctgtga
       61 tctgggttgc catctcagca aaaaagtaga agttttccgc cgcgcgcggg attgcccctt
      121 tcttgatctg gctgaccggc acgccggtat cggcaatctc cacttcggca atctcatcgg
      181 cgtacttctc aattaagtca ccaatcctgc gcagtgcttt tgccctctct tcagctggca
      241 gacgcggcca agggccttga tcgaaggcgg cgcgcgctgc cttaaccgcc cggtcggcgt
      301 ctggcgcgct gccagaagcc acctccgtaa tgggctggtt attggctggg ttgagcgtct
      361 cgaatctgcc cccttcaagc cccggtacaa attcgttatc gataaagtga tttaccacta
      421 aaaaagcctc ctagcattgg ctattcttca gcaaagacgg gattttcaag cacacctagg
      481 ccatcaatct ccaagcgaat cacatcgcct ggatagacat gcgaaatccc tttgggtgtg
      541 ccggtccaga tcatgtctcc ctcctcgagg gtcataatct gagagataaa ttcaatcaac
      601 tctggaactg tcttgatcag gctggctgta ttgcctttct ggcgcagctc cccgttgacg
      661 aaagcgcgca gctccagctt gctggcatca gggatctcgt tcggggtgac taacgttgga
      721 ccaactggtc caaacgagtc ataacccttg gcgcggaccg gcgggcgata gaaattgccg
      781 acgtaatctc gcacggttac atcgttagcg atggtatagc cggcgatgac ctgatctgca
      841 tcctgcctgg agaccttgcg gcagcgcttg ccgatcacaa ccgccaattc cacttcgtaa
      901 tgcatataat caaccccgct tggatagata atgggtgcgc catgccctac cagtgaactg
      961 attggcttaa aaaataaggc tggctcatcg ggtggtgcca tttgcagctc gctggcatgt
     1021 tcggcataat tgagcgccag cccgatcact ttgctgggtt caacgggcgg cagccagacc
     1081 acctcgtcag ggttgtatcg ttcaccctgg cttgagcgca gattgccatc tgggtcgacg
     1141 accccgctat ggagctgacc agcaacgcta aagcgcacca gcctcatgat tcagactcct
     1201 cgcccgcctc ggtgaaaatc gtgctcaaaa aagcccggat gggtgggacg accaccggct
     1261 cctcgctcag caccatggtg taatggttga catctggcag atcgacgcgc cagcatttag
     1321 gaatccagtt gacaaaggcc gccgcctcgc gctcgtccag gacgtgtgct ttgtcaccca
     1381 gcagcccctg gcgcgggcgg atgaacaagc tggggcattg catggtcggg aaatgtacac
     1441 acatgctgta ataaaagtgg atgtccaaat cgcgctcgat ggcttgtggc gacgttttgg
     1501 agcgcacagt gccatcgggc agcgtctcca catcttctcg cagcacccgt tccagcgtgg
     1561 cattccatgg ctgatagtag gggatttctc tcatagtgtt gagatatgcg tccatagatg
     1621 gataactggt acccagacgg cccagcagag ggcgcatggc ttgcatcact tcatctttgg
     1681 gatcgactcc tccgtcgatc atcacgatgc agcgcaagct caatgggcgg atagaggcca
     1741 ggtaaacgcc aaccgtagcc ccaaaggaat gtccgataat gccaaaagtc tgcatgccca
     1801 ttttttcggc aaaagtcagt acgtcgcggg tgtggtaggc aaatccatag ccctcagcgg
     1861 gtttgtcgct gtctccgcgg ccgcgcaggt ctaaggcata caggtggtat tcaggcgaca
     1921 ggcggcttgc aatggcatca aaagagccct tatgcaaggc aaggctaggg aggcaaatca
     1981 agggtccttt ttcaccgggc cattctcgat agctcagttg tataccgttt acctctacgc
     2041 tggaatcggt tgcagtcatg gattacctat aataaatgat actgggcgag cacgcctcgc
     2101 agacgcccaa tgttctcggc gctcattggc gccagcggca ggcggacttc agggctgatc
     2161 ttgcccatca tgcccagggt agttttggcg ggaaccgggt tgatctcgat gaacatcgca
     2221 tcgttaaggg gcatcaaata atagtgcatg tcttgggcct gacgccattt accggcggca
     2281 aacaagtcgt atagctgggc tacctggcga ggcaacacgt tgccggtggc gctcacatag
     2341 cctgcaccgc caattgccag tataggaaag cacagcaatt cgatgccgga gtacaccagg
     2401 aaatcccgtc ccaggcgacc gagcaggcga gtgatatgcg cga
//
LOCUS       DBNHOH010000587         5794 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_355299, whole genome shotgun sequence.
ACCESSION   DBNHOH010000587 DBNHOH010000000
VERSION     DBNHOH010000587.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5794)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5794)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5794
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_355299"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..2243
                     /locus_tag="AC16V7_10225"
     CDS             183..2243
                     /locus_tag="AC16V7_10225"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF015531.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR6257537.1"
                     /translation="MMKNPLNSAFINLRFGLAIAVVFALFALGCQALTNTAIPPTPTP
                     IPEPTPIPTRLPPVPVSPGEENPDEPVFITGDIPYTSPFFINTIFEPFVLLEDQAGFA
                     SRDKEFIFRLEGQAIGPVEIHNDQRVSYSLALPSIPQGTQLDVDNDGKDEVGVQIFAV
                     AYWSNTWGGPFLERRDGKGWSTSYVSTIVDPDREDEIVGGILVVWAPDDQQSFPTGFG
                     EDGLLFTKDDPAAPIPSGYNLVDINQEPFSFYKEARPNITLSEGASAVNDFSTMSYAD
                     AFQAMIEKVERAYPFTREKNISWETLKDEYAARIANVSNDKDFYRVLHDFTQEIPDGH
                     VGMQINGEIFFEDNGGSFGLNLSELSDGRVLVTNVIPNSPGERAGIRLGAELLTWEGK
                     PISEAIDAVNPYFGPYSTQHNRRIGQVLFLTRVPPNTNVTVTFQNPGSNAEQEARMRA
                     DVDYDSLFAAIPELSKDELIIPVEGEILDESGLGYVRVNTFSADYSLIARLWEHFIKG
                     LIDNEIPGLIIDLRVNSGGSGHLATDFAGYFFEEEITLYESLYYSEESNRFEASGLTS
                     KIEPGPLFYAGQIAVLVGPDCVSACEGFAYALTQNGRSTVVGHYPSAGAYGEVGRGQY
                     TLPGDIKMQFPTGRYVMPDGQLALEGVGVIPDVVVPVTEESALGEEDAVLEAAVSTLL
                     ESIK"
     gene            complement(2274..3401)
                     /gene="wecB"
                     /locus_tag="AC16V7_10230"
     CDS             complement(2274..3401)
                     /gene="wecB"
                     /locus_tag="AC16V7_10230"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008735112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HIR6257538.1"
                     /translation="MTKPHRILSILGTRPEVIKLAPVILEMERKPGQFINRVCATAQH
                     RQMLDQTLDVFGLKVHHDLNIMTPGQTISQVTARAVEGLDHVISSERPDLVLVQGDTT
                     TAFCGALAAYYHQVRVGHVEAGLRTGDKYAPYPEEINRRLIGQLADFHFAPTERARQA
                     LLLEGFPQANIFVTGNTVVDALLWVRERVRKAVPELPDGINEWLQDRQMVLVTGHRRE
                     SFGEGFENICQAIRQVADILPEVVFIYPVHLNPNVREPVGRILGGHERIHLIDPLPYE
                     PFVWLMDRATVVLTDSGGIQEEAPSFGKPVLVMREVTERPEGIDAGVARLVGVRQEGI
                     AASLIELLTHPQARQAMAAVQNPYGDGLAAKRITNILERML"
     gene            complement(3398..4423)
                     /locus_tag="AC16V7_10235"
     CDS             complement(3398..4423)
                     /locus_tag="AC16V7_10235"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015149721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR6257539.1"
                     /translation="MEENRLSPKLYRALITGGAGFIGSHLAEALLSRGHHVTVIDNLS
                     TGRFENIQHLIDHPRFRFAIDTITNEVVMDRLVSECDVIFHLAAAVGVMLIVEKPVHT
                     IETNVMGTEAVLKAALRYRAKVLIASTSEVYGKGNHIPFREDDDVVLGPTSRNRWAYA
                     ASKMVDEFLALAYHREKGLPVVIYRLFNTVGPRQTGRYGMVIPRFVQQALRGETLEVY
                     GDGQQSRCFLHVTDAVDAILALADCSEAVGEVFNIGSVEEITIDALARRILDRAQQIT
                     KKSENVSRQIKYVPYDQAYAIGFEDMRQRVPEVTKIAQYTGWKPKRSLEQILLDILED
                     TVKKSTV"
     gene            complement(4423..5487)
                     /gene="gmd"
                     /locus_tag="AC16V7_10240"
     CDS             complement(4423..5487)
                     /gene="gmd"
                     /locus_tag="AC16V7_10240"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR6257540.1"
                     /translation="MKKALITGITGQDGSYLAELLLSKGYEVHGLIRRASTFNTQRID
                     HIYRDPHAQNGVNFHLHYGDIAASGNLIELVYNVQPDEIYHLAAQSHVRVSFDLPEYT
                     GDVTGLGAMRILEAIRKSGIKARFYQASSSEMFGSAPPPQSENTAVEPQSPYAAAKLY
                     AFWVTRNYRQAYDIFASNGILFNHESPRRGETFVTRKITRALAAIKAGKQQYLYLGNL
                     DAKRDWGYAPDYVAAMWKMLQHDQAGDFVIGTGEAHSVREFLDEAFGYLNMDWKDCVK
                     IDPRYYRPTEVDYLLADPGKAQRELGWEPRVFFKDLVHIMVDADLELIGLESPGEGKR
                     ILEQRHGKWHRWESQVVSME"
     gene            complement(5484..>5794)
                     /locus_tag="AC16V7_10245"
     CDS             complement(5484..>5794)
                     /locus_tag="AC16V7_10245"
                     /inference="COORDINATES: protein motif:HMM:NF015665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HIR6257541.1"
                     /translation="IGYLEDQDADCIIHDPFIEDYQGSLLDKAQGCDAAVLMVGHTEY
                     RKLDLKALSGVMKTPVLIDGRSVFDPATAARAGFTYWGVGVHPEAHEKIQMNEVKPIQ
                     "
BASE COUNT         1432 a         1544 c         1386 g         1432 t
ORIGIN      
        1 ttgcctcact gggcttaatg gctggagtga cttggcaatt ggcacgctca tcccttgata
       61 acccggcagg catccttttg ttactaattt caggaactct attggtgaaa tacaatatca
      121 atacaacctg gatgattttc gccggcgctt tggttggata cttattacaa ttaggataac
      181 cgatgatgaa aaaccccctt aactctgcct tcattaacct aagatttgga cttgcgatcg
      241 cagttgtctt tgctcttttt gccttgggct gtcaagctct taccaatact gcaattcccc
      301 cgaccccaac cccgataccc gagcctaccc ccataccgac ccggcttcct cccgttcctg
      361 tcagtccagg cgaagaaaat ccagatgagc cggttttcat tactggagac attccataca
      421 catcgccatt tttcatcaac accatatttg aacccttcgt tctactggag gatcaagctg
      481 gttttgccag cagagataaa gagtttatct tccggctaga agggcaagcc attggacctg
      541 tggagatcca caatgatcag agagtaagct acagcctggc cttgccttct ataccccagg
      601 gaacccaatt ggatgtggac aacgatggaa aggacgaagt cggcgtacaa atcttcgccg
      661 tcgcatactg gtctaatacc tggggtggac ctttccttga aaggcgggat ggcaagggat
      721 ggtcaacttc ttatgtgtct acaatcgttg accctgatcg agaggacgaa attgtaggtg
      781 gtattctggt ggtctgggct cctgacgacc agcaaagctt tcctactgga tttggagagg
      841 atggcttgct gtttacaaag gatgatcccg ctgctcctat cccatcgggt tacaaccttg
      901 tggatatcaa ccaagagcct ttcagttttt acaaagaagc gcgaccaaat atcaccttaa
      961 gcgaaggtgc gagcgcagtc aacgactttt ccacaatgag ctatgcggat gcctttcaag
     1021 ccatgatcga gaaagttgag cgcgcatacc catttacccg ggaaaagaac atctcctggg
     1081 aaacacttaa ggatgagtat gcagctcgca tagctaatgt atccaatgat aaggattttt
     1141 atagagtttt acatgacttt acacaggaaa tccccgacgg gcatgtcggc atgcagatca
     1201 atggagagat tttctttgaa gataatggcg gtagctttgg cctaaactta tctgaactga
     1261 gcgatgggcg tgtattggtg accaatgtaa tcccaaacag ccctggggag cgagccggca
     1321 taaggcttgg agcagagttg ttaacctggg aaggcaagcc aatcagtgaa gccatcgatg
     1381 cggtgaaccc ctatttcgga ccatattcta cacaacataa ccgccgaatt ggacaggtgc
     1441 tcttcttaac ccgcgtgcca cccaatacaa atgttacagt aacgttccag aatccgggca
     1501 gcaacgctga gcaagaagcc cgcatgagag ccgatgtcga ttacgactcc ctgtttgcag
     1561 ccatccctga attgtccaag gatgaactga ttatcccagt cgagggcgag atcctggacg
     1621 aatcaggtct gggttatgtg cgcgtcaata ctttctccgc cgattacagc ctgatcgccc
     1681 gcctttggga acactttatt aaagggctca ttgataatga aatccctggc ttaatcattg
     1741 acctgcgcgt aaactcaggg ggatcgggac atctggcgac ggactttgct ggctacttct
     1801 ttgaagaaga aatcactctt tatgaaagtc tctattacag cgaagaaagt aatcgctttg
     1861 aggcaagcgg cttgactagt aaaatcgaac cgggaccgct tttctatgca gggcaaattg
     1921 cagtgctagt gggcccagac tgcgtcagcg cttgtgaggg atttgcttac gcgctcactc
     1981 aaaatggacg ctctacggtc gtgggtcact accctagtgc aggtgcatat ggcgaagtcg
     2041 ggcgcgggca atacacactc cccggcgata tcaaaatgca gttcccaact gggcggtatg
     2101 taatgccgga tggtcagttg gccctcgagg gcgtgggtgt aatccccgat gtggttgtac
     2161 cggtaactga agaaagtgcc ttaggagaag aggatgcagt cctcgaagcg gctgtgagca
     2221 cgctgttgga gagcattaaa tagtacagga aatacttaaa cagcaaacgc cagttatagc
     2281 attctttcca gaatatttgt gattctcttc gctgcgaggc catcaccgta ggggttctgc
     2341 accgcggcca ttgcctgtcg agcctgtgga tgggttagta actcaatcag gctggcagca
     2401 atcccttctt gacggacacc caccaggcgg gcaacccctg catcgatacc ctccgggcgc
     2461 tcggttacct ccctcatcac caaaaccggt ttgccaaatg aaggcgcttc ttcctggatg
     2521 ccgcccgagt cagtgagcac tacagtggca cggtccatca gccagacgaa gggctcatat
     2581 ggtaaaggat cgatcaagtg gatgcgttca tgcccgccta gaatgcgacc aactggctcg
     2641 cgtacattcg gattgaggtg caccgggtaa ataaatacta cctcgggcaa gatatccgca
     2701 acctgacgaa ttgcctggca gatattctca aatccttcgc cgaagctctc tctccgatgg
     2761 cctgtcacca gaaccatctg tctgtcctgg agccactcat tgatgccatc aggcaattca
     2821 ggaacagctt ttcgtacgcg ttcacgcacc cacagcaagg cgtcaacaac tgtattgccc
     2881 gtgacaaaaa tattagcctg aggaaatcct tctaaaagaa gtgcttggcg cgcccgttcc
     2941 gtgggcgcga agtgaaaatc cgccagctga ccaatcaggc gacgatttat ctcctcggga
     3001 tagggagcat atttatcacc tgtacgcaat cctgcttcaa catgacccac gcgcacttga
     3061 tgatagtatg ctgccagcgc accacagaac gccgtggtag tatcaccttg caccaggaca
     3121 agatccggac gctcgctgga gatcacatga tccagtccct caacggcgcg ggcagtgacc
     3181 tgtgatatcg tctgccctgg ggtcatgata ttcagatcat gatgaacttt caatccaaag
     3241 acatcaagag tttggtcgag catctggcgg tgttgagcgg ttgcgcacac ccggttgata
     3301 aattgacccg gcttgcgctc catctccaaa atgacaggcg ctaacttgat cacctccggc
     3361 cgggtaccca atatactgag aattctatga ggtttggtca aacggtactc ttttttacag
     3421 tatcttcaag aatatccagc aggatttgct ccaagcttcg ttttggcttc caccctgtgt
     3481 attgagcgat cttggtgact tctgggacgc gttggcgcat gtcttcaaaa ccaatggcat
     3541 acgcttgatc ataaggcacg tatttgatct ggcgtgagac attctcactc ttcttggtaa
     3601 tctgctgtgc acgatccagg atcctacgcg ccagcgcatc gatcgtaatt tcttccacag
     3661 acccgatatt aaatacctct cccacggctt ccgagcaatc cgctagagcc aggatcgcat
     3721 ccacggcatc cgtgacatgc agaaaacatc gtgattgttg cccatcgcca taaacctcga
     3781 gggtctctcc gcgcagggct tgttgaacaa aacgaggtat caccatgcca taacgtcccg
     3841 tctgtcgtgg tcccacggtg ttgaataaac gataaataac caccggcaaa cccttctcgc
     3901 gatgataagc gagtgctaaa aactcgtcta ccatcttgga ggccgcgtat gcccagcggt
     3961 ttcgcgaagt aggaccaagc acaacatcgt catcttctcg aaatgggatg tggttacctt
     4021 tgccatacac ctcggaggta gatgcaatta atactttggc tcgatagcgc aatgcggctt
     4081 ttagaacggc ttccgtgccc atcacattgg tctctatggt gtgcacaggt ttctcaacaa
     4141 tcaacatgac acccactgct gctgccaaat gaaagattac atcacactca ctcaccagcc
     4201 gatccatcac cacttcgttg gtgatggtat cgatcgcaaa acgaaagcgc gggtggtcta
     4261 tcagatgctg gatgttctcg aacctccccg tcgataggtt gtcgatcacg gtgacatggt
     4321 gtcctcgtga tagcaacgct tctgctagat gcgatccaat gaagcctgcc ccgccggtga
     4381 tcagggctcg atataattta ggggataatc tattctcttc catcactcca tgctaactac
     4441 ttgactttcc caacggtgcc attttccatg gcgctgctcg agaatgcgct ttccctcccc
     4501 cggagattcg aggccgatta attcaagatc ggcgtccacc ataatatgca ccaggtcttt
     4561 aaagaacacc cttggctccc agcccaactc cctttgagcc tttccggggt ctgccagcaa
     4621 gtaatccact tcagttgggc ggtaatagcg cgggtctatt tttacacaat ctttccagtc
     4681 catgttgaga taaccgaaag cctcatcaag gaactcacgc accgaatggg cctcgcccgt
     4741 gccaatgacg aaatcacccg cctggtcatg ctggagcatc ttccacatag ccgccacgta
     4801 atctggggcg tatccccaat ctctcttggc atccagattg cccagataga gatactgctg
     4861 cttgcccgct ttgatggcgg cgagggcgcg ggtgatcttg cgggtcacaa acgtttcgcc
     4921 acgtcggggc gactcatgat taaataatat cccgttgctg gcaaagatat catatgcctg
     4981 acggtagttg cgagtgaccc aaaaagcata gagttttgcc gctgcgtacg gactctgtgg
     5041 ttcaactgcc gtattctcgc tctgcggtgg tggcgcgcta ccaaacatct cactggacga
     5101 agcctgataa aagcgcgcct tgatgccact cttgcgaatc gcctccaaga tgcgcatggc
     5161 tcccaacccc gtcacatcgc cagtatattc cggtagatca aagctcaccc gtacatgcga
     5221 ctgggcagcc agatggtaga tctcatccgg ttggacattg taaaccagct caatcaagtt
     5281 accagatgcg gcaatatccc cataatgcag gtgaaagtta acgccatttt gagcatgggg
     5341 atcgcggtat atatgatcaa tgcgttgtgt attgaaggtg cttgcccggc gaatcaatcc
     5401 gtgaacctca tatcctttgg aaagcaacaa ttcagccaga taagacccgt cttggcctgt
     5461 gattccagta atcaacgctt ttttcattgg attggtttta cctcattcat ttgaattttt
     5521 tcatgtgctt caggatggac accgaccccc cagtatgtga aaccagctct agcagccgtc
     5581 gctggatcaa aaacagatct cccatcaatt aatacgggtg tcttcatcac accagacaac
     5641 gccttgaggt ccagcttgcg gtattccgtg tgccccacca ttaacactgc tgcatcgcac
     5701 ccctgtgcct tgtccagcaa tgatccctga tagtcttcga taaagggatc atggatgatg
     5761 caatcagcat cctgatcttc cagataccca ataa
//
LOCUS       DBNHOH010000588         2248 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_868604, whole genome shotgun sequence.
ACCESSION   DBNHOH010000588 DBNHOH010000000
VERSION     DBNHOH010000588.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_868604"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..>2248
                     /locus_tag="AC16V7_10250"
     CDS             186..>2248
                     /locus_tag="AC16V7_10250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR6257542.1"
                     /translation="MGCFLRIAIPTLFVLVVISLCLGSYLLFQYNRIKRGLPDVASLR
                     QKASQFETTRILDRNGNVLYEILDPNAGRRTYVPIDKISPYMLAATVATEDKEFYNHP
                     GYDVFAIVRAFWQNYQSGETVSGASTITQQLARNLLFTPEERSLRSYDRKLREAILAS
                     ELTRVYTKDEILELYLNENYYGNLAYGVEAASQTYFGISADKLTLAQASFLAGLPQAP
                     AVYDVYTNRELTLNRQQAVLELMYLASQEQGCIDVSNSPQPVCIDPVSAVSAANELRD
                     YSFRPPEVQIRYPHWVTFIRTLLESQYDPQTIYRSGFSVYTTLDPGLQDQAERVVREQ
                     VDRLADRHATNGALVAIRPTTGEILAMVGSADFYNASISGQVNMALSPRQPGSSIKPI
                     TYVAAFERGWTPSTLIWDVSSEFTPSGKPNDPGPVYKPVNYDDRFHGPVTVRSALANS
                     YNVPAVKTLNFVNIYDDPTTPAQDGFIAMARRLGITTLLRPDYGLSLTLGGGEVSLVE
                     LTGAYAVFANGGRRVPPVAITRILDHLGNQVYQYEPPQGEQVIRSEHAFLISSILSDN
                     QARTPAFGSNSLLNLPFQAAAKTGTTNDFRDNWTLGYTPDVAVGVWVGNADYTPMQNV
                     SGITGAAPIWNEFMQVAILQLTGNRPMPFTRPAGVIEKVICAVSGAEASQWCPRQTTE
                     LFAAD"
BASE COUNT          500 a          696 c          552 g          500 t
ORIGIN      
        1 gtgcaacgcg tgtctcgcct tccgcctacg ctcaacaacg cagggggact cccagcagac
       61 ccatacgacc tccagggacc ccatcaggtc gaccaggagg gccaacctcc ccaagacgag
      121 tagcgacccc tccgcgccgc ccaccccctc gccctcccgc tccgcagatt gacttccgcc
      181 aggggatggg ctgttttctg cgtatcgcta tccccactct ctttgtgctg gtggtcatct
      241 cgctctgcct gggttcatac ctgctctttc aatacaaccg tataaaaaga gggctgccgg
      301 atgtggcctc gcttcgccag aaagcatccc aatttgagac cacgcgcatt ctggatcgca
      361 acggcaacgt cctttacgag atcctggatc cgaatgccgg gcgacgcacc tatgtaccca
      421 tagataagat ctccccctac atgctggctg ctacggttgc aacagaggac aaagagtttt
      481 acaaccatcc aggctacgat gttttcgcca tcgtgcgtgc cttctggcaa aactaccaaa
      541 gcggtgagac agtttcaggt gcatccacca tcacacagca actggcgcgc aacctgctct
      601 ttaccccaga ggagcgctcc cttaggagct atgatcgtaa actgcgcgag gcaattttag
      661 cttccgagct cacgcgcgtc tacaccaaag atgagattct ggagttgtat ttaaatgaga
      721 actactatgg gaatctggcc tatggcgtag aagctgcttc ccaaacctac tttggtatct
      781 ccgccgataa actgacctta gctcaggcat ccttcttggc tggtctgcct caggcaccgg
      841 ctgtatacga tgtatacacc aaccgcgagt tgactttaaa tcgccagcaa gccgtgctgg
      901 aattgatgta cctggcaagc caggagcaag gctgcattga tgtcagcaac agccctcaac
      961 cggtgtgcat cgatcctgta tctgcggtca gtgctgccaa cgaactgagg gattactctt
     1021 ttcgaccccc cgaggtgcag atccgctacc cacactgggt aacgtttata cgcactttgc
     1081 ttgagtcaca gtacgatcca caaacgatct accgctccgg attctcagtt tacaccactt
     1141 tggatccagg tctgcaagat caggctgagc gggtcgtgcg cgaacaggta gatcgtttag
     1201 cagaccgtca tgctaccaat ggcgcattgg tagcaatccg acctacaacc ggtgaaatac
     1261 tggcgatggt aggctctgcc gacttctaca atgcctccat atctggccag gtgaatatgg
     1321 cccttagccc caggcagccc gggtcatcca ttaaaccaat cacctatgtt gctgcctttg
     1381 agagagggtg gacaccttcc acgctgatct gggacgtgtc ttccgagttc accccctccg
     1441 gtaagcccaa cgaccctggt ccagtgtaca agccggtcaa ttacgatgat cgattccatg
     1501 gcccggtcac ggttcgttcc gccctggcaa actcttacaa cgtccctgca gtaaagacgc
     1561 taaacttcgt caacatttac gatgatccga ccacccctgc ccaggatggc tttatcgcta
     1621 tggcgcgccg cctgggcatc accactttgc tacgccccga ttacggcctc tcgctcacct
     1681 tgggaggcgg ggaagtctcg ttggtggagt taaccggtgc ttatgcggtc ttcgccaatg
     1741 gcgggcggcg tgtcccacca gtagccatca cccgcatctt agatcacctg ggcaatcaag
     1801 tgtatcaata cgaaccccca caaggggagc aagtcatccg cagcgagcat gccttcctaa
     1861 tctcctcgat cctctcggat aaccaggcgc gcactccggc ttttggatcc aactcactcc
     1921 tcaacctgcc attccaagca gctgctaaga ccggcactac caacgatttt cgggacaact
     1981 ggacactggg ttatacacca gatgtcgccg ttggggtatg ggttggtaac gccgattaca
     2041 cccctatgca gaatgtaagc ggcatcactg gtgctgcgcc aatctggaac gagttcatgc
     2101 aggttgccat cctgcaactc accggcaaca ggccaatgcc cttcacccgt cctgcaggag
     2161 tgattgaaaa ggtgatttgc gcggtatctg gcgccgaagc ctcgcagtgg tgtcccaggc
     2221 agacgacaga actctttgcc gcagatca
//
LOCUS       DBNHOH010000589         1260 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_933382, whole genome shotgun sequence.
ACCESSION   DBNHOH010000589 DBNHOH010000000
VERSION     DBNHOH010000589.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_933382"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            355..>1260
                     /locus_tag="AC16V7_10255"
     CDS             355..>1260
                     /locus_tag="AC16V7_10255"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR6257543.1"
                     /translation="MVDPDISKYASLLKQQYLFQGLNDAQLAHVVTHVERIEKFKDDV
                     IYAQGSPGDKFYVIFQGRVRVTQKEGQRERQLRILNAGDYFGEGGLLFKRPRSDTITA
                     LEPTVLLRLSREDFFELLEIMPSIRMNLSATAESRYLALKENFDWLSDDEVIYLIRRK
                     HELFLLISLIPPVILGLIALPIFVLSFSQTSPSLTIAALLFGIFGMIGSVMWGIWNWL
                     DWGNDFYIVTSQRVVWLERVIFFYYSRQEAPLTQVLSVNVKTSFIGRIFNYGSVDVRT
                     FTGGIPMQNMTDPKQFASYVEGFQSR"
BASE COUNT          340 a          298 c          289 g          333 t
ORIGIN      
        1 aaacagataa atccggcgtg ctttcaagcg aggtcggacg aatcgttgaa gtctgcgtgg
       61 gttcaacgat tgtgcttgtt gccaggctga aatctgtaga cttaggggtc gctatctccc
      121 cagaaggagt gaattgacaa gcgggtaaac ccaaaacaag aatgctgcca aggaggaata
      181 tacagaacct gaatcgatga tacatcaagt tattcctaat acaatggcaa ttttcttgca
      241 cccataacca tgcttagccg aagtcaaatc aaatatattt ttattgtagt cctttttcga
      301 gttttgtaaa atcgaatcgc ccaatacaag aaaagttcgt gctaaaatca ctgcatggtc
      361 gatcctgata tctccaaata tgcttccctg ttgaagcaac aatacctgtt ccaagggcta
      421 aacgacgccc aactagccca cgtcgtcacc catgttgagc gcatcgaaaa gttcaaagat
      481 gacgtcattt atgcacaggg atcgcctgga gataaattct acgtgatctt ccagggcagg
      541 gtgcgtgtca cacaaaaaga aggtcaacgg gagcgccaat taaggatact taacgcagga
      601 gattattttg gcgagggtgg cttgctattt aaacgcccac gatcagacac aatcactgcc
      661 ctagagccca cggtattact tcgcctgagc cgtgaagatt tctttgaatt gctcgaaatc
      721 atgccgagca tccgcatgaa cctctccgca accgccgaga gtcgctacct agctctgaaa
      781 gagaatttcg actggttgag cgatgatgag gtcatctacc taattcgacg caagcacgag
      841 ttgtttctac ttatctctct aatcccacca gtgatactgg gactcattgc gctaccgatc
      901 tttgtgctta gcttctcgca aaccagtcct tccctgacaa ttgctgcgct cctttttggc
      961 atatttggga tgatcggttc ggtcatgtgg ggaatttgga attggctaga ttggggaaac
     1021 gacttctaca ttgtcaccag ccagcgcgtg gtgtggctgg aaagggttat ctttttttac
     1081 tatagccgcc aggaagcccc gctcacacag gtgctctctg tcaatgtcaa aacaagcttt
     1141 attgggcgaa tatttaatta tgggagtgtc gatgtacgca ccttcacggg tggcattccc
     1201 atgcaaaata tgacagaccc caaacaattt gcttcctatg ttgagggatt ccaatcgcgc
//
LOCUS       DBNHOH010000590         1212 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1702198, whole genome shotgun sequence.
ACCESSION   DBNHOH010000590 DBNHOH010000000
VERSION     DBNHOH010000590.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1702198"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="AC16V7_10260"
     CDS             complement(<1..1197)
                     /locus_tag="AC16V7_10260"
                     /inference="COORDINATES: protein motif:HMM:NF019869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR6257544.1"
                     /translation="MIMMGLEFTGEIPFSTVYLHGLIRDEVGRKMSKTYGNVIDPLTV
                     MDELGTDALRFTLLVGSTPGNDMNLSIKRVEANRNFANKIWNVGRFVISSIQEFESDN
                     RWNAEREKSSSPIPYPSHTWTVADRWIYARLAALISDVERLFQNYQYGEAGRQIYEFF
                     WNEFADWYIEIAKLQLASKAGDGSGAVDREGAHVSPAANDTKSITAHMLVYVLDTCLR
                     LLHPFTPFVTEELWGHLKRALGQTSLSYQMSSEALIVARWPEPPTREPGEKEAVEQFS
                     LIQDVVRAIRNLRAEKSVKPGKRIPALINAGDRAQVLRAQAEAIASLAQLDEQALNIQ
                     ESIASREEGTIALVVSGIEVYLPLAGLVNLDEERSRLEKELADMERQRKRVQELLSSP
                     FAQKAPP"
BASE COUNT          297 a          330 c          273 g          312 t
ORIGIN      
        1 cggtggtgct ttttgcgcaa aggggctcga cagcaattct tgcactcttt ttcgttggcg
       61 ttccatatct gctagttctt tctccaggcg agatcgttct tcatccaggt tcaccagccc
      121 cgctagaggc aggtatactt cgataccact cactaccagt gcgatggtcc cttcttcacg
      181 cgaggcaatt gactcttgga tattcaatgc ctgttcatca agctgggcta gggaggcgat
      241 tgcctccgcc tgagcgcgca atacctgtgc tctgtcaccg gcgttgatca gtgcagggat
      301 acgctttcct ggcttcacac tcttttcagc ccgcaaattg cgaatagcgc gcaccacatc
      361 ttgtatcagg gagaattgct cgaccgcttc tttttcgccg ggttctcgcg tgggaggttc
      421 aggccaccta gccacaatta gagcttcaga agacatttgg taactcaatg aagtttggcc
      481 aagagcgcgc ttcaggtgtc cccatagctc ctctgttaca aatggtgtaa aaggatggag
      541 aagccgcaag caggtatcca aaacatacac gagcatatga gcagtaatgg attttgtatc
      601 attggcggca ggtgaaacat gcgcaccttc ccgatctacc gcacccgagc catctccggc
      661 cttactagct aactgcagct tggcaatctc gatgtaccaa tcagcaaact cattccagaa
      721 gaattcgtag atctgcctgc cagcctcgcc atattggtaa ttctgaaaaa ggcgctccac
      781 atcgcttatc aaagctgcca atcgagcata aatccagcga tctgctacgg tccaagtatg
      841 cgaaggatat ggaattggag acgaagattt ctctcgctct gcattccatc tattatctga
      901 ttcgaactct tgaattgagg agatcacaaa cctgcctaca ttccagattt tattagcaaa
      961 attgcgattg gcctccacgc gcttgatact taggttcatg tcgtttcctg gtgttgatcc
     1021 cactaataag gtgaaacgca gagcatccgt ccccagctca tccatgacag ttagcggatc
     1081 aatcacattc ccgtaagtct tgctcatctt gcgaccaacc tcatctcgga taaggccatg
     1141 caaatacaca gtggaaaagg ggatctcgcc ggtaaattcc aaccccatca tgatcatgcg
     1201 agccacccag aa
//
LOCUS       DBNHOH010000591         7097 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_804393, whole genome shotgun sequence.
ACCESSION   DBNHOH010000591 DBNHOH010000000
VERSION     DBNHOH010000591.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7097)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7097)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7097
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_804393"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="AC16V7_10265"
     CDS             complement(<1..235)
                     /locus_tag="AC16V7_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HIR6257545.1"
                     /translation="MELHTTWIYLSPHFDDVAISCGGLAWEQAQRGNRVEVWTICAGD
                     IPPGAISPFAESLHKRWQTSQNTPAQRRDEDLAS"
     gene            complement(460..837)
                     /locus_tag="AC16V7_10270"
     CDS             complement(460..837)
                     /locus_tag="AC16V7_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257546.1"
                     /translation="MSDVSVGVIVSVGVRVSVGVEVGVAVGVGEGGGVVGDGVAVGDT
                     KTVFVTVGVAVAVLVSVGVLVAVAVGVNVPKAGKGESVAVGKGGGVIVIVGVAVMVGV
                     KVIVAGGNAPRSGAMLAANSPRQ"
     gene            953..2008
                     /locus_tag="AC16V7_10275"
     CDS             953..2008
                     /locus_tag="AC16V7_10275"
                     /inference="COORDINATES: protein motif:HMM:NF017181.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR6257547.1"
                     /translation="MTELLELLKRLISAPGLSGYEAPVREIIAEAWRPLADEVSTSKL
                     GSLHALKRGTAPEPRPSLLIATHMDAIGLMVTSISGEFLHITQVGGVDPRVLPGQLVT
                     VHGRRDLPGVIAQPPTHLLPPNNRNGPVALDDLLVDTGLAAGELSRLVRIGDLVSFAQ
                     MPLEMRGDIIAGHTLDNRASVAALTACLEELQKRQLAWDLWAVASSQEEETFGGAFTS
                     AFQLRPSLAVALDVTFASSPGTPSHQAVDMGKGVAIGLGPNIHPALHKAFKELAGRLE
                     IAYKVIVLETYSGTDAYAMQVSAEGIPTMVLYIPLRYMHTPVEMVSIKDIHRAGRLLA
                     EFAVSVDAEFMDKLTWD"
     gene            2081..3154
                     /locus_tag="AC16V7_10280"
     CDS             2081..3154
                     /locus_tag="AC16V7_10280"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017181.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIR6257548.1"
                     /translation="MMTPNSDKKSPKIGAAQIRLLERLTQACAVSGDEGEVRAIVLEH
                     IRPVADDVRVDALGNVLATRRGQVEPRLRVLLTAHMDEVGFILTHDEDNGFFRFERVG
                     GIDVRYLPGKSVWVGKDHLSGVIGAKPIHLATKEELRQTISQDTLRIDVGPGNGSKVK
                     PGDRATFATSFTRLGASLRAKALADRLGVATLIELVKHAPANIDLLAAFTVQEEVGLR
                     GAGVAAYALNPDLAIILDSTPANDLPAWDESENTKYNTRLGAGPAIYMADRGTISDPR
                     LVRHLVETAEAAGIPYQIRQPGGGGTDAGAVHKQRAGIPSVSVSVPARYNHTPAAIAR
                     LSDWKNTLALLHAALKQLTAEIL"
     gene            3151..4146
                     /locus_tag="AC16V7_10285"
     CDS             3151..4146
                     /locus_tag="AC16V7_10285"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIR6257549.1"
                     /translation="MKELIQTLVEIPGPSGYESKIREAIRLQIEPLVDETRIDALGNL
                     IARKGQSQGDGQKIMLAAHMDEIGLIVTHVDENGFARFTTLGGVRAQNLPGGRVRFMN
                     GASGVIGVQYHGEPKASAFEEMFIDLGAKNRADCPVRVGDVAAFERPFTDLGGRLVAK
                     AMDDRIGVAVLIETLRHLNSSPHEIYFVFSVQEEVGVRGATTAAFGVDPDIGLAVDVT
                     RTGDTPRDKKMEVSLGKGPAVKVRDGGMLSDPRVVDWMVRTAEKARLPYQIEILDGGS
                     TDARAIQVSRAGVPAGCLSIPCRYVHSPSEMVDYSDVQNGVQLMVALLSGPIELG"
     gene            4151..5428
                     /locus_tag="AC16V7_10290"
     CDS             4151..5428
                     /locus_tag="AC16V7_10290"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR6257550.1"
                     /translation="MVKRSLILVLLWLFVAGCTATPTPVITGVPTVTATPTATQPPAP
                     RITPTPDRQLTLEVWLPPQFDTVTGTVAGDLLQARLDQFATEQADVIVQMRIKPLDGP
                     GGLLDSLTTASAAAPLALPDLVALPRPVLETAALKGLLRPFDDLVNNLEDTDWYDYAL
                     QLSQLQDSHFGQPFAGDALVLVYRKEIVPEPPSTLTYTLHSEGPLIFPAGDPQALYTL
                     ALYQAAGGSILDDEGRPFLEEEPLLEVLSYYHEAALNEFTPFWLTQFQSDDQAWDAFQ
                     TGQANMVVTWASRYLKNKSEDIQVVPIPTLDGKNFTLATGWVWALASPFPERQRMAAE
                     LAIFLSDSEFLAQWTEATGYLPPRPSALSDWNTAGLRAIVEDVSSSAYLYPSADVLPG
                     LAAPLSEATILMLKQQRDPASAAQEAVNRLLGP"
     gene            5488..6732
                     /locus_tag="AC16V7_10295"
     CDS             5488..6732
                     /locus_tag="AC16V7_10295"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HIR6257551.1"
                     /translation="MRSPEEESRLGELSVAEDVPSRIPSQDTPETGSSPATTERNWFL
                     EQWERLSQAGLAESVLRLGTHTLLLALILLVAWAMRQFYRLGETQEAQQDAALAAPLP
                     TPTPTEPPPQLPLFDPQAMEASGIMRLAILDTMIPARPRTVVDKYIVEKGDTIFGIAE
                     KFNLQPETILWGNQFALADNPHNLRPDQELNILPIDGTYYQWQAGDGLNGVSKFFGVK
                     PEDIINYPGNQLNPDEIGDFAHPNIEAGTWLIVPGGRREFVTWSAPAIPRDNPGVAKV
                     LGPGACGSVVDGAVGVGAFIWPSNNHFLSGFDYSPASNHPGVDIDGNTGDAVYAADNG
                     VVVYAGWNNWGYGYVVVLNHGNGWQTLYAHLSAYNVGCGQSVYQGTLIGAIGSTGNSS
                     GSHLHYEMMYQGTKVNPWDYLP"
     gene            complement(6729..6878)
                     /locus_tag="AC16V7_10300"
     CDS             complement(6729..6878)
                     /locus_tag="AC16V7_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257552.1"
                     /translation="MMNEKRDAGIPFFDVFLFNRNAHLPTYADLYVRRIATEGAGEGE
                     EGIFV"
     gene            complement(6906..>7097)
                     /locus_tag="AC16V7_10305"
     CDS             complement(6906..>7097)
                     /locus_tag="AC16V7_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257553.1"
                     /translation="VQEYISAIEAAGFVDVKLNPVYFEPQTINEAIDQVGEAFDLKDI
                     PSESLRKTVFSAKITAIKP"
BASE COUNT         1541 a         2008 c         1895 g         1653 t
ORIGIN      
        1 acgaagccaa atcctcatca cggcgttgag cgggtgtatt ttggctggtc tgccagcgct
       61 tatgaaggct ttcagcgaag ggcgagattg ctcccggcgg tatatctccg gcacagattg
      121 tccatacctc gacgcggttc ccgcgctgcg cttgttccca ggcaagcccg ccgcaggaga
      181 tggccacgtc atcgaagtga ggggagagat atatccaggt cgtatgcaat tccattcagg
      241 ttgatgataa taacatattt tgctccatct ccctcatata tgctatcttc actagggcaa
      301 ttgcgcatgc ccctcagggt atatgcaatt gccctgtatc ttcacttgtc accttacccc
      361 tattggtgtt atcatcaagc ccgacatgct ctctgacccg agcgcccgca ggcgtctgcg
      421 tcttacgctg atttctattg ttttggctac tatcccttgt tactgccttg ggctatttgc
      481 tgccagcatg gcacccgagc gcggcgcgtt gcccccagcg actataacct tcacacccac
      541 cattactgcc acccccacaa tcacgatgac cccaccccct ttgccaactg cgacgctctc
      601 acccttccct gctttgggca catttacccc caccgccact gccaccagca ctcctacgct
      661 aaccaatact gccacagcca ctccgacggt cacgaatacc gttttcgtgt ctcccacagc
      721 cactccatcc cccacaacac cgcccccttc accaacgcca accgccactc ccacctctac
      781 ccctacggag accctgacac ccactgagac gataacccca accgagacat ctgacacgtc
      841 actctaggtt atacagagga aaaggataaa cattcgcatt cggtatgcag ttgacagcat
      901 attctaaata aggatgccat ctggctaccg gcttatttca tcaattgcaa aaatgactga
      961 attacttgaa ttactcaaaa ggctaatttc tgcccctggc ttgagcggtt acgaggcgcc
     1021 cgtgcgcgag atcatcgctg aagcctggcg accactggcg gatgaggtca gtaccagcaa
     1081 gctgggcagc ttgcacgccc tgaaacgtgg aactgctcct gagccacgac ctagcctgct
     1141 tattgctacc cacatggatg ccattggatt gatggtcacc agcatctccg gcgagttctt
     1201 gcatatcacc caggtaggtg gtgtcgatcc ccgtgtgctg cctgggcagt tggtcacggt
     1261 gcacggacgt agagacctcc ccggagtgat cgcccagccg cctacacacc tgctcccgcc
     1321 gaataatcgc aatggaccgg tggcgctgga tgatctgttg gtggataccg ggttggcggc
     1381 gggtgaatta agtcgcctgg tacgtattgg agatctggtt tcatttgctc agatgcccct
     1441 cgagatgcgc ggcgatatta tagctggaca taccctggat aaccgcgcct cagttgctgc
     1501 cctgaccgca tgcctggagg aactgcagaa acgccagctt gcctgggatt tgtgggctgt
     1561 agccagctcg caagaggagg agacttttgg cggcgcgttc acctcagcat tccagctccg
     1621 tccatcgctg gcggtagctt tagatgtcac ctttgccagc agcccgggta cgccttcaca
     1681 tcaggctgtt gacatgggta agggtgttgc catcggtctt ggtccaaaca ttcatccagc
     1741 tctacacaag gcattcaagg agcttgctgg gcgattggaa atcgcataca aagtgatcgt
     1801 gttggaaacc tattccggga ctgatgcata tgccatgcag gtgtcggcag agggtattcc
     1861 aacgatggtc ttatacatcc ctctgcgcta tatgcacaca ccggtggaga tggtatctat
     1921 taaagacatt catcgcgctg gacgcttgtt ggccgagttt gccgttagcg tagatgctga
     1981 gtttatggac aagctgacct gggattgacc ctgtgccagt tgccaatgat tatgcaacac
     2041 tcagtttggt acacatggcc catgatatgt gaattgtaaa atgatgacac ctaacagcga
     2101 taaaaaatcc ccaaagatcg gcgcagccca aatccgtttg ctggaacgcc tgacacaagc
     2161 ctgcgccgtc tccggcgacg agggcgaggt gcgcgccatt gttctggaac acattcgtcc
     2221 tgtcgcggat gacgtgcgag tagatgcctt gggcaacgtg ctggctacgc ggcgcggcca
     2281 ggtagagcct cgcctgcggg tcttattaac tgctcacatg gacgaagtgg gttttatcct
     2341 cacgcatgat gaagacaacg gcttcttccg cttcgagagg gtgggcggta tcgatgtgcg
     2401 ctacttgccc ggcaagtcag tttgggtagg aaaagaccat ttgtccggcg taatcggtgc
     2461 caaaccgatc caccttgcaa ccaaggagga gctaaggcag acgatatcac aggatacgct
     2521 gcgcatcgat gtcggacccg gtaatgggtc gaaggtcaag ccaggcgaca gggctacttt
     2581 tgctacgtct ttcactcgac taggtgccag cttgcgcgct aaagcgctgg ccgatcgact
     2641 gggtgtggct accctgatcg aactggtcaa gcatgctccg gcgaacattg atctcctggc
     2701 ggctttcacc gtccaggagg aagtaggttt gcgaggtgct ggtgttgctg catatgccct
     2761 gaacccagat ttggctatta ttctcgatag cacccctgct aacgacctgc ctgcctggga
     2821 cgaatcggag aacacaaagt acaacacgcg actcggcgcc ggcccggcga tctacatggc
     2881 tgaccggggc acgatttccg atccgcgctt ggtgcgccac ttggttgaga ccgctgaggc
     2941 tgctggtatc ccgtatcaaa ttcgccagcc cggcgggggt ggcacagatg ccggggcggt
     3001 gcacaagcag cgtgccggaa taccctcggt gtccgtctct gtaccggcgc gctataacca
     3061 taccccggcg gccattgccc gcttatcgga ttggaagaat actctggcgc tattgcatgc
     3121 cgcgctcaaa cagcttacag cggagatcct atgaaagaac tgatacaaac cttggtggaa
     3181 atacctggcc cttcaggcta tgaatccaaa attcgcgagg ctatccgcct tcagattgag
     3241 ccgctggtgg atgagacgcg tatagatgcc ctgggtaacc tgatcgcccg caaagggcaa
     3301 tcccaggggg atggccagaa aatcatgctg gccgcccaca tggatgagat aggtttgatt
     3361 gtgacccatg tggatgagaa tggttttgct cgctttacca cgctcggggg cgtgcgagcc
     3421 cagaatcttc ctgggggtag ggtgcgtttc atgaatggcg cctcgggcgt gatcggcgtg
     3481 caatatcacg gcgagcccaa agcctcggct ttcgaagaga tgttcatcga cctgggcgca
     3541 aaaaatcggg cagattgccc ggtgcgagtg ggtgatgtcg ctgcctttga gcgcccattc
     3601 accgacctgg gcggacgact ggtagccaag gcgatggacg atcgcatcgg ggttgccgta
     3661 ttgatcgaga cactgcgcca tttaaattca tcgccgcatg agatctattt cgtcttcagt
     3721 gtgcaggagg aggttggcgt gcgtggggct accacagccg cattcggtgt agacccagat
     3781 attggcctgg ctgtggatgt aacccgcacc ggcgacactc ctagggataa aaagatggag
     3841 gttagcttgg gaaaaggccc agctgtcaag gtgcgtgatg gtggcatgct cagcgaccca
     3901 cgcgtggtgg attggatggt gcggactgcc gaaaaagcac ggctgcccta ccagatcgaa
     3961 atattagatg gaggtagcac agatgcccgt gctatccagg tctcaagagc gggtgttccc
     4021 gcaggatgcc tatccatccc atgtcgctat gttcactcgc cctctgaaat ggtcgattac
     4081 agcgatgttc agaatggtgt ccaactgatg gtcgcgctgc tcagcgggcc aattgaacta
     4141 ggataggttg atggtaaaac gaagtttgat tctggttttg ctatggctct tcgtggcggg
     4201 ttgtactgcg acgcccacgc cggtaattac cggcgttccc actgtgacag ctacccccac
     4261 agccacgcag ccgcccgctc ccaggatcac ccctacccca gacaggcaac tgaccctgga
     4321 agtctggttg ccacctcagt ttgacaccgt gacaggcacg gtcgctggag atctgctcca
     4381 ggcccggctg gatcaatttg ctacagaaca agcagatgtg atagtgcaaa tgcgcatcaa
     4441 acctctagat ggaccgggtg gattgctcga ttcgctcacc accgccagcg cagctgcgcc
     4501 gctggccttg ccagacctgg tcgctttgcc ccgtcctgtg ctggaaacgg ctgccctcaa
     4561 ggggcttctg cgcccatttg acgacctggt caataacctt gaggatactg attggtatga
     4621 ttatgccttg cagctgtccc agctccaaga tagtcatttt gggcagccct ttgctggcga
     4681 cgcgttagtc ctggtctacc gcaaagaaat tgtacctgaa cctccttcaa cattaaccta
     4741 caccttacat agcgaaggac ctctaatatt tcctgcaggt gacccgcagg ctttatacac
     4801 tcttgcgctc taccaggcgg caggcgggtc catattggat gacgaaggac gtcccttttt
     4861 ggaagaagaa ccgctcctag aggtgctgtc ctattaccat gaggctgctc tcaatgagtt
     4921 cacacctttc tggcttaccc agtttcagag cgatgatcag gcctgggatg ctttccaaac
     4981 aggccaggcc aatatggttg tcacctgggc ttcgcgttat ctgaagaata agtcggaaga
     5041 cattcaggtt gtccccatac cgactctgga cggcaaaaat ttcaccttgg ctacaggctg
     5101 ggtttgggcc ttggctagtc ctttccctga gcgccagcgg atggctgccg aactggcaat
     5161 atttcttagt gatagtgagt ttctggcaca atggacggaa gctactggct acttgccgcc
     5221 tcgccctagt gcattgagtg actggaatac tgcgggactg cgtgccatcg ttgaagatgt
     5281 ttcttcgtct gcctatctct acccttctgc ggatgtactc cctggcttgg cagcgccgct
     5341 gtccgaggct accatcctga tgctaaaaca gcagcgcgat cccgcctcgg cggctcaaga
     5401 ggctgtaaat cgtctccttg ggccttaatc ccacaatttt cctggtttga cgaaaacccc
     5461 tgccagtgtt attatccgtg cacaaatatg agatcgcctg aagaagaatc ccgcctcggc
     5521 gaactttctg tcgccgagga tgtgccttcc cggataccct ctcaagatac accagagaca
     5581 ggctcctccc ccgcaacaac ggaaaggaac tggtttctgg aacaatggga gcgcttatcg
     5641 caagcgggtt tggccgagtc agtgctccgc ttggggacgc acacattact actagctttg
     5701 atactgctcg ttgcatgggc tatgcggcaa ttctatcgcc tgggagagac acaagaagcc
     5761 cagcaggatg ccgcgttagc cgcaccgctg cctaccccga cgccgacaga accccctcct
     5821 cagttgccgc tctttgatcc ccaagcgatg gaagcctcgg ggataatgcg gctagcgatt
     5881 ctggatacta tgatcccagc tcggccgcgc actgtagtgg ataaatacat cgtagagaag
     5941 ggcgatacaa tattcgggat cgccgaaaaa ttcaatcttc agcctgagac catcctatgg
     6001 ggaaatcaat ttgccctggc tgataacccc cataacctgc gcccagatca ggagctaaac
     6061 atcctgccaa ttgacggcac ttattaccaa tggcaagcag gcgacgggct aaacggcgtt
     6121 tccaagttct ttggtgtcaa gcccgaagat ataatcaatt accctggtaa tcaactgaac
     6181 ccggatgaaa tcggtgattt tgcccacccc aacatagaag ccggcacatg gctgatcgta
     6241 ccgggtggtc ggcgcgaatt cgttacctgg agcgccccag ccatcccgcg cgataaccca
     6301 ggcgtagcca aggtgcttgg tcctggggct tgcggctcag ttgtggatgg tgctgttggt
     6361 gtgggtgcct ttatctggcc ctcaaataac cacttcctgt ctggctttga ttactctcct
     6421 gcttctaatc atcctggcgt tgatattgac ggaaacactg gcgatgcggt gtacgcagcc
     6481 gataatggcg ttgtcgtcta tgctggatgg aataactggg gctacggtta tgtcgtggtt
     6541 ctcaaccatg ggaacggctg gcagaccctt tatgctcacc tgagcgccta caatgtaggc
     6601 tgcgggcaga gcgtctatca aggaactttg attggagcca tcggtagcac cggaaactcc
     6661 tctggctcgc acctgcacta cgagatgatg taccagggca ccaaggtgaa tccctgggat
     6721 tacctcccct aaacgaaaat cccttcctca ccctccccag ctccctcagt tgctatcctc
     6781 ctgacataga ggtctgcata ggttggcagg tgggcgttcc tgttgaatag gaagacatca
     6841 aaaaacggga tgccagcatc ccgtttttcg ttcatcatcc cctccccatc tgggtaatcc
     6901 aaaaattatg gttttatcgc tgtgattttt gcgctgaaca ccgtcttgcg cagcgactca
     6961 ctgggaatgt ctttcaggtc aaacgcctcg ccgacctggt caatggcttc attaatcgtt
     7021 tgtggctcaa agtagaccgg atttagcttc acatccacaa agccggcagc ttcgatggcg
     7081 gagatgtatt cctggac
//
LOCUS       DBNHOH010000592        10957 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_99164, whole genome shotgun sequence.
ACCESSION   DBNHOH010000592 DBNHOH010000000
VERSION     DBNHOH010000592.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10957)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10957)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10957
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_99164"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1003
                     /locus_tag="AC16V7_10310"
     CDS             44..1003
                     /locus_tag="AC16V7_10310"
                     /inference="COORDINATES: protein motif:HMM:NF013648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6257554.1"
                     /translation="MHAIRNRRRVVALLCMGFILSACASATPAPTQLPAATPTPTGIS
                     IVDGMGREITLDRPAQKVASLAPSNTEILFAIGAGAQVIARDSFSDFPEQALAVADIG
                     GGFGEIDTETLVSLEPDLVLAAEINPVENVRMLEDLGLKVFYLSNPKELDGLYENLRT
                     VARLVGHEAETEGLIESLKLRVSTIEEKVSSLQERPLVFYELDGTDPSAPWTSGPGTF
                     IDLLLTKAGGDNLGNTLDSEWVQISVEELITQNPDIILLGDYTWGGITPEDIAARPGW
                     EVIAAVQNKQVYTFDDNLVSRPGPRMVDGLEALAELLHPELFQ"
     gene            1004..2032
                     /locus_tag="AC16V7_10315"
     CDS             1004..2032
                     /locus_tag="AC16V7_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013615609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIR6257555.1"
                     /translation="MNISPRPILINLALLVIAALLSIGVGPVFIPPDTVTRMLVDRLP
                     WIAINPDWPEAFSAILFQIRLPHTALMLLTGAALGGSGAAYQGLFRNPLADPYLIGVA
                     SGAALGAVAALTIQWPSSLMGMYTLPASAFLGAMITITVVYNLARVGRAVPTTTLILA
                     GVAFSAFATSLMSFLMLRSNTEMRRALTWLLGGAIMSGWQPVMAMLPYELIGLGILAA
                     SGHALNVMQFGEEQAQQLGLPIERIKLFLIVAASITTAAAVSFSGVIGFVGLVAPHVV
                     RLLWGPDYRRLTPLAILAGGSALLLADMLARIVLAPQILPVGIVTALAGAPFFLWILR
                     RAKTQAYW"
     gene            2045..2854
                     /locus_tag="AC16V7_10320"
     CDS             2045..2854
                     /locus_tag="AC16V7_10320"
                     /inference="COORDINATES: protein motif:HMM:NF010068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HIR6257556.1"
                     /translation="MLNIHSLTVAYQERIVLDDLSLEVARGEILAVVGPNGVGKSTLV
                     RALSGVLPPVSGQVNLENRDLTQLSPMQRARLMAVVPQARELPGAYTVYQTVLLGRTP
                     YLSWLGHAGTGDHAAVRVALEQTRTLDLAERPVGELSGGEQQLVLLARALAQDAPVLL
                     LDEPTTHLDLAHQSNLLNLVRRLSKEKNLTVLMVLHDLNLASLYADRVALMVSGRIHA
                     IGKPSEVLTTDNLSAVYQVAVNVIPHPDYGNPLILPDGLNSLEAGETVVTH"
     gene            2865..3899
                     /gene="cobT"
                     /locus_tag="AC16V7_10325"
     CDS             2865..3899
                     /gene="cobT"
                     /locus_tag="AC16V7_10325"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019423049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HIR6257557.1"
                     /translation="MIQPLDKGAMQSARSRQDRLTKPQGSLGQLEALSVHIAGITGQS
                     QPHLEHKVVVVMAGDHGVARQGVSAYPPDVTQQMVLNFLRGGAAINVLARHSGARVVV
                     VDMGVAADLPPHPDLKIKKIAYGTENIATGAAMSRSQAEQAIRNGADVILEEIARGLD
                     ILATGDMGIGNTTPSAAIAVSITGLPVESIVGRGTGIDEAGLKRKVAAVRSALEVNQP
                     NPHDGLDVLAKVGGFEIAGLVGAILAAAAHRRPVVIDGFISTAAAMIAVSLCPLAGGY
                     LIASHASQELGHRHMLESLQLKPLLDLELRLGEGTGAVLAMSIVEAACKILSEMATFD
                     EAGVSEKSAG"
     gene            3903..4685
                     /gene="cobS"
                     /locus_tag="AC16V7_10330"
                     /pseudo
     CDS             3903..4685
                     /gene="cobS"
                     /locus_tag="AC16V7_10330"
                     /inference="COORDINATES: protein motif:HMM:NF014685.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
     gene            4667..5227
                     /gene="cobU"
                     /locus_tag="AC16V7_10335"
     CDS             4667..5227
                     /gene="cobU"
                     /locus_tag="AC16V7_10335"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015236102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HIR6257558.1"
                     /translation="MGNLTLILGGARSGKSTFALNLAEKIRGGVAFIATAQAGDPEMA
                     ARIAAHQRQRPADWRTLEIPHAIAATWLGQEIDADLIILDCLTLLVSNLAVMASPDVD
                     HPNEGKFTELVRSEINTLIDLIQNSPGDWIVISNEVGLGLVPPYPLGRIYRDWLGWAN
                     QRLATVASEVYLLVAGIPVPIHNYRL"
     gene            complement(5261..6034)
                     /gene="pgl"
                     /locus_tag="AC16V7_10340"
     CDS             complement(5261..6034)
                     /gene="pgl"
                     /locus_tag="AC16V7_10340"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HIR6257559.1"
                     /translation="MSGKSSASAINSKTHVFQNIEVLAGGAAGYILSAMQETLAVQDR
                     FAMMLSGGSTPRAVYTLLASSPYKQAVDWSQVHIFWGDERCVPPDHPDSNYRMAGEVL
                     ISKVDLPAENVHRIRGEIPPDQAAASYEIAISSYFGSETAFPHFDLILLGMGEDGHIA
                     SLFPGSPAINETARWVVAVDHDQPPPPLVSRVTVTLPVINAARRVALIVAGEAKASTV
                     AATLTTPEKSLVLPVQRVNPVEGELVWLLDEAAAGQITY"
     gene            complement(6003..6641)
                     /locus_tag="AC16V7_10345"
     CDS             complement(6003..6641)
                     /locus_tag="AC16V7_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR6257560.1"
                     /translation="MLKSVFGRRDDEERIKQEGRLPPGQSLTNRFPVLHYGPTPRFNP
                     ATWDLRVFGEVEEEVVWNWDEFNKLPRTKISMDIHCVTRWSKFDTEWEGVSIKTLIDQ
                     GYIKLKPTARHLMQHCEYGFTVNIPLEVALAPNFLLATHFNGEPLEPDHGYPLRGVVG
                     NIPGTDFTTPYFWKGGKWIRGLEFMAQDRLGFWEQAGYHNEADVWKEQRFGY"
     gene            7044..7880
                     /locus_tag="AC16V7_10350"
     CDS             7044..7880
                     /locus_tag="AC16V7_10350"
                     /inference="COORDINATES: protein motif:HMM:NF013539.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIR6257561.1"
                     /translation="MLYEDRIRRERAEMSKSFAKLADFLLNSYIEAAFMTASELAHTL
                     DLDAATVVRFSQFLGYSGFPELQREIRSRVKKDLLIRPKQAEDDNSTPGIVSRAMQEL
                     NLALEQTRITLDTDAVERLVEQIGNVRRTVVLAEGPAQPTAYSLVHYFEQGGFPIYIA
                     RPGIADLARTVNTATSQDLFLAIEVAGQSPYIARALGEAKAKNIPTAAIVGASSLASA
                     RAADIVLAAHAHPSLGVGILTIEAIVYAFAQVLRLRYADNFVGTEQAIAELTARIQTN
                     TD"
     gene            7885..8655
                     /locus_tag="AC16V7_10355"
     CDS             7885..8655
                     /locus_tag="AC16V7_10355"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR6257562.1"
                     /translation="MRIIRYQTQNDPPQYGWVLENAVGAIEGDPFGEFRRLEANQNLD
                     SVRLLAPVLPGKIICVGRNYAAHAREHDAEVPEVPLLFLKAPSAVIGPGDKIVLPPQS
                     QQVEHEAELAVVIGRRGRWISPEEALEHVMGYTIGNDVTARDLQFRDNQWTRAKGFDT
                     FCPLGPWIQTDFDPTDTLITCHVNGEMRQMASTRDMVFSVRQLIAFTSSIMTLEPGDI
                     IMTGTPSGVSPLQPGDTVKVSIEGLGELENSVVSQPPR"
     gene            8655..9683
                     /locus_tag="AC16V7_10360"
     CDS             8655..9683
                     /locus_tag="AC16V7_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257563.1"
                     /translation="MPEGIRLPISLDIHTAVQTALVIVGLLIVLFFWLGIRSIRTARR
                     LRFFRMRRDRMVRGWRLIITAIILIPIAILLNRRLEPLIYTYYQPTVTVTQTPTITIT
                     PTISQTPTITLTPTITLTPLVSNTPTITPTPFIPPAIEARFESTITPNPESVFSPLTF
                     TDGLDALYRPLKEGTVFENPVEHMYAVFSYDGMIRGSQWTALWYRNGELVHFETLPWD
                     GETGGLGYTDWQPQPEEWFTGTYEVQIFVGRDWKVSGTFTVEGEPPTPVPTATPTTTP
                     TSTYTPTSTKTRLPTRTYTPIPPTRPPYISPTPTKTPTPRPTLTRTPITPSITPQPTR
                     TRTVTPSP"
     gene            complement(9757..10740)
                     /locus_tag="AC16V7_10365"
     CDS             complement(9757..10740)
                     /locus_tag="AC16V7_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003334710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HIR6257564.1"
                     /translation="MTNWLSSSSKIKKLPPYFSLTQEVTHALELNLPLVALETTVITH
                     GLPYPDNLTLADNMEDEVRNQGVTPATIGVIDGRVQVGMVPAQLSFLATSGGELLKIS
                     LRDFAPAIASLASGGTTVAGTMYAAHAVGIHVFATGGIGGVHFELSSRRKGAFDVSAD
                     LPALANIPMIVVCAGAKAILDLQATLEYLETWGVPVVGYQTDDFPAFYSSRSGLKTST
                     RADTPEEVAFVARSHWALGMKSAILVTVPPPDEVALPSEAVKKAVRAALKAAQTEKIS
                     GKEVTPFLLSRVSELTEGSSLRANLGLLLNNANVASQIARALSRMEREGIV"
     gene            complement(10721..>10957)
                     /locus_tag="AC16V7_10370"
     CDS             complement(10721..>10957)
                     /locus_tag="AC16V7_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR6257565.1"
                     /translation="CYATSESSGYIPAIKTSILDPTGAGDALTATVIFALLNDIPLDD
                     AVRLGVSAASLTLRFRGAVVPDLSLEKLYDQLVI"
BASE COUNT         2493 a         3105 c         2867 g         2492 t
ORIGIN      
        1 ggattttgct taatcaactt agttttataa acgaggagaa tcgatgcatg ccataagaaa
       61 ccgccggcgg gttgtcgccc tgctgtgtat gggctttatc ctgagcgcct gcgcttcagc
      121 cacacccgcc cccacgcagc ttcctgctgc aacccccaca ccaaccggca tatccattgt
      181 tgacgggatg ggtagagaga ttaccctgga tagaccagcc cagaaagtgg cctcattggc
      241 cccctccaat acagagatcc tttttgctat cggtgcaggc gcacaggtga tcgctcgcga
      301 tagcttttcc gattttcctg agcaagcctt agcggttgcg gatatcggtg gcgggtttgg
      361 agagatcgat acagagactc tggtctcgct cgagcccgac ctggtgttag cagccgagat
      421 caacccggtt gagaatgtgc gaatgcttga agatcttggt ctcaaagtat tctatctctc
      481 gaatccgaaa gaattagatg ggttgtacga gaatcttcgc actgtagccc gcctggtagg
      541 gcacgaggct gaaaccgaag gactcattga gagcctaaaa ctgcgcgttt ctaccatcga
      601 ggagaaggta agtagccttc aagagcgccc cctggtcttc tacgagctgg atgggaccga
      661 tcccagcgcc ccctggacat ctggcccggg gacattcatc gacctgttgt tgaccaaagc
      721 tggtggtgac aacctgggca ataccctgga cagcgaatgg gtccaaatca gcgtggagga
      781 gctaattacc caaaatcccg acatcatatt gctgggtgat tacacctggg gcggcatcac
      841 acctgaagat atcgccgctc gccccggatg ggaggtaatc gctgccgtgc agaataagca
      901 agtgtatacc tttgatgaca acctggtcag ccgtccaggc ccgcgcatgg tagatggatt
      961 ggaagctctg gcagagctgc tgcacccgga actgttccaa tagatgaaca tctctccacg
     1021 gcccattttg attaacttgg ccctgctcgt catcgctgcg ctgctgagca tcggcgtagg
     1081 gccggttttc atcccacctg atacagtgac ccgcatgctg gtggaccggt tgccgtggat
     1141 agccatcaac ccggattggc ccgaagcctt ctcggctatc cttttccaaa ttcgcctgcc
     1201 acacacagct ttgatgctac tcacaggcgc ggcgctgggg ggcagtggcg ccgcctacca
     1261 gggtctgttc cgcaatccat tagcagatcc atatctgatc ggtgtggcgt ctggagcagc
     1321 attgggcgct gtagccgctc taaccatcca atggcccagc tcactcatgg gcatgtacac
     1381 attgccagcc agcgcctttc tcggtgctat gatcacgatc actgtggtat ataacctggc
     1441 tcgcgtgggg cgagcggtgc ccaccacaac gctcattctg gcgggagtgg cattcagtgc
     1501 attcgccacc tcgttgatgt catttctcat gctgcgctcc aacacagaga tgcgccgtgc
     1561 ccttacatgg ctgctggggg gcgccatcat gagcgggtgg cagcctgtca tggctatgtt
     1621 gccctacgag ttgatcggtt tgggcatcct ggcagcttct ggacacgctc taaatgtgat
     1681 gcagtttggc gaggagcaag cacagcagtt aggcctgccc atcgagcgca tcaagttgtt
     1741 cctcatcgta gccgcctcta tcacgaccgc cgcggcggtt tccttttcgg gggtcatcgg
     1801 cttcgtcggc ttggtagcgc cgcatgtcgt ccgcctgtta tggggacctg attaccgcag
     1861 gctcacaccc ctggcgatcc ttgcaggggg cagcgccctc ttgctagccg atatgctggc
     1921 gcgcatcgtc ttggcacccc aaattttgcc cgtgggcatc gtcacagccc tggctggcgc
     1981 accctttttc ttgtggatct tgcgccgggc gaagacacaa gcctattggt aaacaggagg
     2041 tcccatgtta aatatccatt ccttaaccgt agcgtatcag gaacgcatcg tgctggacga
     2101 ccttagcttg gaagtagccc gtggtgagat cttagctgtg gttggaccca acggggtagg
     2161 caaatccacc ctggtgcgcg ccttgagcgg tgtgctcccg cctgtgagtg gtcaggtgaa
     2221 tttagaaaat agagacctca cccaactttc gcctatgcag cgtgcacgcc tgatggctgt
     2281 cgtgccccag gcgcgcgagt tgcccggtgc ctacacggtc taccaaaccg tgctgctagg
     2341 gcgcacgccg tatttgagct ggttaggaca cgctgggaca ggcgatcacg ctgctgtgcg
     2401 ggtcgcttta gagcaaaccc gcacgctgga tctagctgaa cgacctgtgg gagagctctc
     2461 tgggggtgag caacagctgg tgcttctggc gcgagccctg gcgcaggatg cacccgtgct
     2521 gctgcttgat gaacccacca ctcacttaga cctagcccac cagtccaatt tgctcaacct
     2581 ggtgcgccgc ttatccaaag aaaagaattt aaccgtcctg atggtattgc acgatctgaa
     2641 cctggcaagc ctgtatgcag atcgggtggc gcttatggtc tcaggtcgca tccacgccat
     2701 aggcaagccc tccgaagtgc taaccactga caacctgtct gctgtttacc aggttgcggt
     2761 caacgtgatc cctcatcctg attatggcaa cccgctaatt ctgccggatg gattaaactc
     2821 cttagaagcc ggagaaaccg ttgtcactca ctgaaattct ggcaatgatc cagcctctgg
     2881 ataagggcgc catgcaatct gcgcgctccc gccaggatag gttaacgaaa ccacagggca
     2941 gtctggggca actagaggca ttatctgtac atatagcggg catcacaggc cagtcgcaac
     3001 ctcatctgga gcataaagtt gtcgtcgtga tggctggcga ccacggggtg gctcgacagg
     3061 gtgtgagcgc ttacccgccg gacgttacac agcagatggt gctgaatttt ctgcgaggcg
     3121 gcgcggccat taatgttctt gctcgtcatt ctggggcgcg tgtggtggtg gttgatatgg
     3181 gcgtagccgc tgatttgccg ccgcatccag acctgaaaat caagaagatt gcctatggaa
     3241 ccgaaaacat cgccactgga gcggcaatga gccgctcaca ggctgagcag gctatccgaa
     3301 atggggcaga tgtgatactc gaagagatcg ctcgcgggct ggacatcctg gcaacgggtg
     3361 acatgggcat tggaaatacc accccctccg ccgccatcgc cgtgtcaata accggactac
     3421 ccgtcgagtc tatcgtcggt cgcggcactg gtatcgacga agccgggctg aaacgaaagg
     3481 tcgccgcagt acggagcgct ctggaagtta atcagcccaa tccccatgat ggattggatg
     3541 ttctggctaa agttggcgga tttgagattg ccgggttggt gggcgcaatc ctggcagccg
     3601 cagcacatcg ccgtccagta gtgatcgatg ggtttatctc cactgcggcg gccatgatcg
     3661 ccgtcagcct ttgcccgctg gcaggcggtt acttgatcgc ctctcacgcg tcgcaggaat
     3721 tgggtcacag gcacatgctg gaatcgttgc agcttaaacc gctgcttgac ctggagctgc
     3781 gcttgggtga aggaaccgga gccgttttag cgatgtcgat tgtcgaagcc gcctgtaaaa
     3841 tcctgagcga aatggccaca ttcgatgaag ccggcgtatc cgaaaaaagt gcagggtgat
     3901 agatgcaaga tgaaggcaca caggatacga aagggcgcgc acctttacct ttgccagggg
     3961 tgggattcct ggcagccgtc cagtgtctca ccatcgctcc cccgatcatt aggcgcccct
     4021 tttctgccag ggaaatcggc ggggctgtag cttatttccc gctggtaggt gcaatacttg
     4081 gagcactcct ggcaatatct gacgccctcc tggcggggat cttcccgtct cctgtgcgga
     4141 gcgccttatt gctctctcta tgggtactgc taactggagc cctgcatctg gatggcttcc
     4201 tggacagcct ggatggcctc ctcggaggcg gcacgcccga aaatagattg gcgatcatgc
     4261 gggatgagcg tgtgggcgca tatgccctgg caggaggaat cttattgttg ttgctgaaat
     4321 tcagcgcact gctttccatg ccagagcgtt gggctgcact agtcatcgca ccgacctaga
     4381 gtcgatgggg tatgagcctg gcaatcgtcg ctttcccgta tgcccgatca gagggattgg
     4441 ggcgggttat gaaagatcac gctacctggc tgcatgccag cgcagccgca ttgacagccc
     4501 tggccatcgc ctggttctat gggcaacgga gcggattgat tctgcttgca gtatctgctc
     4561 tcaccgtaat cctggtagcg cgctttgttt taaaacgtat tcccggcttg accggcgata
     4621 tctatggcgc gctcaatgag cttctcgagg tcttgacctt actcacatgg gaaatcttac
     4681 attaatcctt ggcggggcgc gcagcggcaa gtccaccttt gccctaaatc tggcggaaaa
     4741 aatcaggggg ggtgtggcat tcatcgctac cgctcaggcc ggtgacccgg agatggcagc
     4801 tcgtattgct gctcaccaac gccagcgtcc agccgattgg cgcacgctag agattcccca
     4861 tgcgatcgcc gcaacttggc tggggcaaga aatagatgcc gatctgatca tcctggactg
     4921 cctgaccctg ttggtttcta acctggccgt aatggcctct cccgatgtag atcatccaaa
     4981 tgaggggaaa ttcaccgagc tggtacgcag cgagatcaac acgctgatcg acctgattca
     5041 aaacagcccc ggggattgga ttgtcatttc gaatgaggtg gggttaggcc tggtgccacc
     5101 atatccatta ggtcgcattt accgagattg gctaggttgg gccaatcagc gcctcgccac
     5161 tgtcgcttct gaggtttacc tgctggtggc gggtataccc gtgcccattc acaactatag
     5221 gttatgaaat gcccaggaga aaacctgacc gcagccccat ctagtaagtt atctgtcctg
     5281 ccgcggcttc gtccaacaac cacactaatt ccccctctac cggattaacc ctttgcacgg
     5341 gcaaaacgag ggacttttcc ggggttgtta gcgtcgcagc cactgtagac gctttggctt
     5401 cgccggcaac aatcagagcc acgcggcggg cggcgttgat gaccggtaag gtaaccgtca
     5461 cgcggcttac caatggtggc ggcggttgat catgatccac agccacgacc cagcgcgcag
     5521 tttcattaat tgccgggctt cctgggaaga gcgaggcgat atgaccatcc tcccccatgc
     5581 ccagcaagat caagtcgaag tgagggaaag ctgtttcact gccgaaatag cttgaaattg
     5641 caatctcata actggcggct gcctgatccg gcggtatttc accccgaatg cggtgaacgt
     5701 tttcggcagg cagatctacc ttcgagatca atacctcgcc tgccatgcgg taattgctat
     5761 cagggtgatc cggtggtaca cagcgctcat cgccccagaa gatgtgcacc tgagaccagt
     5821 ccactgcctg cttatacggc gagctagcaa gcagagtgta caccgctctc ggcgtggagc
     5881 caccggatag catcatggcg aagcgatcct gcaccgccag cgtctcttgc atggctgata
     5941 agatgtagcc ggcagcccca ccagctaaca cctcaatatt ctgaaatacg tgggtctttg
     6001 aattaatagc cgaagcgctg ctctttccag acatctgcct cgttatggta gcctgcctgc
     6061 tcccaaaaac ccagccggtc ttgcgccatg aactctagtc cgcgaatcca tttaccaccc
     6121 ttccagaagt atggcgtcgt aaaatcggtt ccagggatat tgcccacaac gcctcttagc
     6181 gggtagccgt ggtctggctc gagtggctca ccgttgaagt gtgtcgccag cagaaaattg
     6241 ggcgccaatg ccacctcgag agggatattc acagtaaaac catattcgca atgctgcatc
     6301 aggtgccttg cggttggctt gagtttgatg tagccctgat caatcagggt tttgatcgag
     6361 acaccctccc actcggtatc aaatttgctc cagcgcgtca cgcagtggat atccatagat
     6421 atcttggtgc gaggcagctt attgaactca tcccagttcc aaaccacctc ctcttcgacc
     6481 tctccaaata cacgcaaatc ccaggtcgcg gggttaaaac gcggcgttgg gccataatgc
     6541 aacaccggga aacggttggt taatgactgt cctggtggta agcgaccctc ctgcttgatc
     6601 cgttcttcat catctcgacg tccaaatacg ctttttagca taacaacctc cacaattatt
     6661 gcataagttg ggcattcatt gcttcatttc aatgagctcg atacgccccg caaaagacac
     6721 agtaaataca aaatgccaag gcgacggtta atacagcagt catgtcgttt gacagcgatt
     6781 atctctttgc tgtgtaatta cttagacaac cttctctaat tggattatac ctgtcaagaa
     6841 agaacttgtg ggcacacagc gattaacaaa atattacaat ctatcgtaaa aatcgagatt
     6901 ttgtactcga ttttattctt gggattaagc gtaaatcaat taagctacaa tagttgttga
     6961 gactagcgaa ttcatcacaa acatttgcat atacaaggtg gttatgataa acttagaaaa
     7021 tagttgcaag tgaggaggaa gctatgttat atgaagaccg catccgtcgt gaacgagcag
     7081 agatgtccaa aagcttcgcc aagctggcgg attttttgct taattcatac atagaagccg
     7141 cattcatgac cgccagcgaa cttgcccaca ccctggattt agacgcagct acggttgtcc
     7201 gcttttcaca atttcttggc tactctggct tccccgagct acaacgcgaa atacgcagcc
     7261 gcgtgaagaa agacctgctg atccgcccta agcaagcgga ggacgacaat tcaacaccag
     7321 ggattgtctc cagagcgatg caggagttaa atctggcact cgaacagaca cgcatcaccc
     7381 tggatactga tgccgtggaa cgccttgtgg aacagattgg caacgtcagg cggaccgtgg
     7441 tgctagccga aggccctgcc caacccactg cctacagcct ggtgcactac tttgagcagg
     7501 ggggttttcc catatatatt gcacgacccg gcatcgctga cctggcgcgc accgtcaata
     7561 ccgccacctc tcaagaccta ttcctggcaa ttgaggttgc gggacaatcc ccttatatcg
     7621 cacgcgcctt gggcgaagcc aaggcaaaaa acatccctac cgcagctatt gtaggcgctt
     7681 catctctggc ttccgcacgt gccgcggata tcgtactcgc agcacatgct cacccctcat
     7741 tgggggttgg catactaacc atcgaggcaa tcgtgtacgc atttgcccag gtgctgcgcc
     7801 tgcggtatgc cgataatttt gttggaaccg aacaagccat cgcggaactt accgcgcgaa
     7861 ttcaaacaaa caccgattaa tactatgcgc atcatacgct accaaaccca gaacgacccc
     7921 ccacaatacg gctgggtgct tgaaaatgca gttggagcca ttgaaggcga ccccttcgga
     7981 gaattccggc gcctggaagc taaccaaaat ctcgactcgg tgcgtctact ggctcctgta
     8041 ctgccaggga agatcatctg cgttgggcgc aattacgcag cccacgcccg ggaacacgac
     8101 gctgaagttc cagaggtgcc cctgttattc ctcaaagcgc cttcagcggt gattggacca
     8161 ggcgataaaa ttgtccttcc accccaatcg cagcaggtcg agcatgaagc cgagctagca
     8221 gtggtcatag gtcggcgcgg gcggtggata tcccccgagg aagccctgga acacgtcatg
     8281 gggtatacta ttggcaatga tgtcaccgca cgcgatctcc aatttcgtga taaccagtgg
     8341 acgcgcgcca aaggctttga cactttctgt cccctaggac cctggatcca gactgatttt
     8401 gaccctaccg acactctcat cacctgccat gtcaacgggg agatgcgcca aatggcttcc
     8461 actcgagata tggtctttag cgtccgccag cttatcgcat ttacatcttc catcatgact
     8521 ttggagccgg gtgatatcat catgactggc acgccgtctg gggtcagccc gttacagccc
     8581 ggagatacgg tcaaagtctc gattgaaggt ttgggtgaat tggagaatag cgtagtctcc
     8641 caaccacctc gctaatgccc gaaggaattc gtcttccgat atctctcgat atccataccg
     8701 cagtccagac cgcactggtc attgtgggac tgctgatcgt gctattcttc tggctaggga
     8761 ttcgctcgat ccgcactgca cggcgtctgc gcttctttcg aatgcgccgc gaccgaatgg
     8821 tgcgcggctg gcgtttgatc atcaccgcca tcatcttgat ccctatcgct atcctgttga
     8881 atcgtcggtt ggaacctttg atatatactt attaccaacc tacggtcacg gtaacgcaaa
     8941 cgcctactat cacgattaca cccaccataa gtcaaacgcc aaccattacc ctgacaccaa
     9001 ccatcacgct gacgcctctc gtctctaaca caccgacaat cacacccacg ccatttatac
     9061 cgcccgcaat agaggctcgc tttgaaagca cgatcacacc caaccctgaa tcagtattca
     9121 gcccgctaac cttcactgat gggctggatg ctctctaccg acccctcaag gagggcacag
     9181 tttttgaaaa tcccgtcgag catatgtacg ctgtcttcag ctatgatggc atgattaggg
     9241 ggtcgcaatg gacagcttta tggtatcgta acggggagct ggttcatttt gagactttac
     9301 cttgggatgg cgagacgggt ggtctgggtt acacagactg gcagccccaa cccgaagaat
     9361 ggtttacggg aacttacgag gtgcagatat tcgttggtag ggattggaag gtatccggta
     9421 ctttcactgt agaaggtgaa ccacctacac cagtgccaac cgccacgcca accaccacgc
     9481 cgacctccac atacacaccc acgtcaacaa aaactcgtct tccaacccgc acgtacacac
     9541 ccatcccccc aacgaggcca ccctacatta gccccacacc gacaaaaacc ccgaccccca
     9601 ggcccacatt gacgcgcacg cctatcaccc caagcatcac cccccagcca acccgaacca
     9661 gaacagttac cccgtcaccc tagctcgtga tttaattatc caagagttct gcacaacccg
     9721 tctctttttc tactgcttca aatccccact tgaaaattaa acgatcccct cccgctccat
     9781 gcgggatagg gcgcgggcga tctggctggc gacattggcg ttgtttaaca gcaagccaag
     9841 attggcgcgc aagctggatc cctcagtcaa ttcggatacc ctgctcaaca aaaagggggt
     9901 aacttctttt ccggatatct tttctgtctg agctgctttt agagccgctc gcaccgcttt
     9961 tttcactgcc tcgctaggca aggcgacttc atctggcggt ggaacagtga ccagtatggc
    10021 gcttttcata ccgagtgccc aatgtgagcg tgccacgaat gcaacttcct cgggagtatc
    10081 tgcgcgagtg ctggttttca gcccgctacg gctagaataa aaagcgggga aatcgtctgt
    10141 ctggtagcca accactggca ccccccaggt ttctagatat tccaaagttg cttgtagatc
    10201 aaggatggct ttggcgcccg cgcacacaac aatcatgggg atgttcgcca gggcgggtaa
    10261 atctgcagag acatcaaaag ctcctttgcg ccgggagctg agctcaaaat gcaccccacc
    10321 aatacccccg gtagcaaaaa catgtatccc aaccgcgtgg gcagcataca tggtgccagc
    10381 cactgtcgtt ccaccgctag ccagggaagc aatcgccggg gcgaagtcac gcaggctaat
    10441 tttgagcaat tcaccacctg aggtcgccaa aaagctcaat tgagctggca ccatacccac
    10501 ctgcacgcgt ccatcaatca cgccgatagt tgcaggtgtg acgccctggt tgcgcacttc
    10561 atcctccata ttatctgcca gagtgaggtt atcgggatat ggcaaaccgt gggtgatgac
    10621 ggtggtctca agggctacca aaggaagatt aagctccaag gcgtgcgtaa cctcttgagt
    10681 gagcgagaaa tatgggggca atttttttat cttcgaggag ctagataacc aattggtcat
    10741 acaatttttc cagggacaaa tccggaacca ctgcgccgcg gaaacgtaac gtgagagatg
    10801 ccgcagaaac accaagccgc actgcatcat caagtgggat gtcgtttaac aatgcaaaaa
    10861 tcacagtcgc cgtgagtgca tcaccagcac ccgttggatc taaaatggat gttttgatgg
    10921 ctgggatata accagaactc tccgaagtcg cataaca
//
LOCUS       DBNHOH010000593         2413 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1446785, whole genome shotgun sequence.
ACCESSION   DBNHOH010000593 DBNHOH010000000
VERSION     DBNHOH010000593.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1446785"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="AC16V7_10375"
     CDS             <1..1022
                     /locus_tag="AC16V7_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257566.1"
                     /translation="LQVSSQPEPVLPTNTSIELSVPSTTETSQSQEIAVIHLPLDGPI
                     ADPLAEISGMAWYADDLLLLPQYPDRFGDDGNGYIFGLNKKDILSYIQGATGNPLTPY
                     LIPLITNNLEAMLNGFEGFEAIAVEGDQIYLTVETKPAGMMGYLVSGKISPDLSHIIL
                     DTSRLISITPQTELTNYSDEALLVFGNRLFSIYEVNGAALNPTPLAHQFDFSLQVLDP
                     LVFPNIEYRITDATSVDENGRFWGINYFFPGESALKPDADPLEHQYGEGPSHSRFETI
                     ERLVEFQFSYSGIILSDTPPIQLELIDDNHSRNWEAIARLEGFGFLLSTDKFPETILG
                     YIPYP"
     gene            1048..1998
                     /locus_tag="AC16V7_10380"
     CDS             1048..1998
                     /locus_tag="AC16V7_10380"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF002320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIR6257567.1"
                     /translation="MSGHPLRIFSGSSHPELASEIATQLGVPLGRSTTIRLPDSETHI
                     MIDEVVRDQDIFFIQTCPAPVNDHIMELLLYLDAFRRASAHDISVVIPYFPYARQDRM
                     ARGREAISARVVASLIESMGASRVIFVDIHNRAIQGFFNIPVDPLSALPMLANYFRKP
                     EFNDAAIVSPDVGRTTMAGHYAELLNLPLVVMHKRRTSISHTETTHVVGDISGRRPII
                     IDDLLAGGSVLRQIDALYDRGATGKAYFSITHPILLPTALQILDEDERIEKLIVTNTL
                     PVPPEKRHPKLEIISIAPLLADIISHIHQGLSISGKLVLE"
     gene            2009..>2413
                     /locus_tag="AC16V7_10385"
     CDS             2009..>2413
                     /locus_tag="AC16V7_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6257568.1"
                     /translation="MSKPSNYRWFVVGVFFLFMLLHQADKLLIGPLTTPIMRDFGIDE
                     VQMGAVFTGALLVGAFLYPIWGYLYDRFARAKLLALASFIWGSTTWISAIAPTFPTFL
                     ASRASTGIDDSSYPGMYSLISDYFGPAVRGKVY"
BASE COUNT          587 a          658 c          545 g          623 t
ORIGIN      
        1 cgttacaggt gtcatcccag cccgagcctg tcctccccac caatacatcc atagaattat
       61 ccgtaccttc gacgaccgaa acttcccaat cgcaagaaat cgcggtaata cacctaccgc
      121 ttgacggccc aatcgcagac cctctggctg aaatctccgg aatggcgtgg tatgcagacg
      181 atttacttct gctcccccaa tacccagatc gctttggtga tgatggaaat ggttatattt
      241 tcggtttgaa taaaaaagat atcctcagct atatccaagg ggcaacgggt aatcctttaa
      301 ccccgtacct tatcccgctc attacgaata atcttgaagc catgctgaac ggctttgaag
      361 gctttgaagc catcgccgtc gaaggagacc agatttactt gactgtcgag acaaaacctg
      421 ctggaatgat gggatatctg gtttccggaa aaatatcccc cgatttgagc cacattatcc
      481 tggatacatc tcgcttgatc tccatcacac cgcaaactga attaactaat tacagcgatg
      541 aagccttgct ggtttttgga aaccggcttt tcagcatcta cgaggtcaac ggcgctgctc
      601 tcaaccctac cccgcttgcc catcagttcg acttctcatt gcaagtactg gatccattgg
      661 ttttcccaaa tattgaatat cgaattacgg atgctacatc agtcgatgaa aatggccggt
      721 tttgggggat aaactatttc tttcctggcg aaagtgccct gaaacctgat gccgacccac
      781 tagagcacca atatggcgaa ggtccctcgc atagccgttt tgaaactatt gagcgcctgg
      841 tcgaattcca gttcagctat tcgggtatca tcttatcgga tactccacca atccaactgg
      901 agctaatcga tgacaaccac agtcgcaact gggaggcaat tgcccgcctc gaaggttttg
      961 gctttttact ctctactgac aaatttccgg aaaccatcct gggatatatc ccctacccat
     1021 aaccgggtat aatctgttgt atttcctatg tcaggacacc cactacgcat ctttagcgga
     1081 tcgtcacatc ccgagctggc cagcgagatc gccacgcaac tcggcgtccc gctcggcagg
     1141 tcaaccacga ttcgcctccc cgatagtgaa acccatatca tgatcgatga ggtagtgagg
     1201 gatcaagata tcttctttat ccaaacctgc ccagcgccag taaacgatca catcatggag
     1261 ctgcttctct atctcgacgc atttcgtcgc gccagcgccc atgacattag cgtggtcatt
     1321 ccatacttcc cttatgcccg ccaggatcgc atggcgcgcg ggagagaagc tatcagcgcg
     1381 cgcgtcgttg cctcgttgat tgagagcatg ggagccagcc gggtaatctt tgtagacatc
     1441 cacaaccgtg ctattcaagg attttttaat atccccgtag atcctctgag cgcgctgcct
     1501 atgttagcaa actatttccg caagccggag tttaatgacg cggcgattgt cagcccagat
     1561 gttgggcgca cgacgatggc aggccattat gcagagctgc tcaacttgcc cctggtggta
     1621 atgcacaagc gcaggactag catcagccat actgaaacca cccacgtggt gggggacatt
     1681 agtggccgtc gacctatcat catagatgac ttgctcgctg ggggcagcgt gttgcgccag
     1741 atcgacgctc tctacgaccg tggcgctacc gggaaagcgt atttctctat cacccacccc
     1801 atcttgcttc caaccgcgct tcaaatcctg gatgaggatg agcgcattga aaaactgatt
     1861 gtgaccaaca ccttgcctgt gccaccagaa aagcgccatc caaagctcga aatcatatct
     1921 attgcccctt tgcttgctga tatcatcagc cacattcacc aaggattgag tatctcaggg
     1981 aaattagtcc tggaatagga gaagtaagat gagcaaacct tccaattata gatggtttgt
     2041 tgttggggta tttttcttat tcatgctgct ccatcaagct gataagttgt tgatcggccc
     2101 tttaaccacg ccaatcatga gagattttgg aattgatgaa gtgcaaatgg gagctgtttt
     2161 taccggagca ctgctggttg gggctttttt atatcccata tggggatatt tatatgaccg
     2221 cttcgctcgc gccaagctgt tagccctggc atcatttatt tggggatcaa ctacctggat
     2281 aagtgcaatt gcccccactt tccccacctt tctcgccagc cgcgcttcaa ctggcataga
     2341 tgactcgagc tatccgggta tgtacagcct gatctcagat tacttcgggc ccgctgtgcg
     2401 cggtaaagtc tac
//
LOCUS       DBNHOH010000594         7150 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_292225, whole genome shotgun sequence.
ACCESSION   DBNHOH010000594 DBNHOH010000000
VERSION     DBNHOH010000594.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7150)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7150)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7150
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_292225"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="AC16V7_10390"
     CDS             complement(<1..202)
                     /locus_tag="AC16V7_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HIR6257569.1"
                     /translation="MRTDQTATTNIDEYMAGFPNEVREILEKIRMTIREVAPDADETI
                     KYGIPTFTLKGNLVHFAAYKKHI"
     gene            complement(260..904)
                     /locus_tag="AC16V7_10395"
     CDS             complement(260..904)
                     /locus_tag="AC16V7_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005372887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0043934 - sporulation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HIR6257570.1"
                     /translation="MYLPKQFEETQFHVMFELIRAHPLATLVTLSSGGLNANHIPLSL
                     SESPPPYGTLRGHIARANPLWSDLNSDIESLAVFSGPDTYISPSWYATKKETGKVVPT
                     WNYAVVHAYGILRIVDDASWLRSQLEALTIHNEASFSEPWAVSDAPQDFTERLISSIV
                     GVEMIITRLSGKWKVSQNQPPQNQASVVASLKASDIPDAPSMATLVETRSKNAR"
     gene            complement(1226..2002)
                     /locus_tag="AC16V7_10400"
     CDS             complement(1226..2002)
                     /locus_tag="AC16V7_10400"
                     /EC_number="2.8.3.-"
                     /EC_number="4.1.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003332833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase subunit beta"
                     /protein_id="HIR6257571.1"
                     /translation="MTVTTAAYTSTELLACVAAKLLEDKKSVLVGTGLPMIAALLAQK
                     THAPNLLLIFEAGGIGPRMSQMPISVGDILTFYKAVAATSMHDVMSASQAGYVDYGFV
                     GAAMIDMYGNINTTVIGDWERPKTRLPGSGGANDLGSFCHRTIIIMRQDKRRFVEKID
                     FITTPGYLDGPGARERAGLPEGTGPYRIITQLGLYGFDEQTKRVKLLALHPGVTLDDV
                     RANTSFEILIPEMVQVTHPPTEEELRLLHEMDPIGVIVRK"
     gene            complement(2111..3124)
                     /locus_tag="AC16V7_10405"
     CDS             complement(2111..3124)
                     /locus_tag="AC16V7_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007143367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HIR6257572.1"
                     /translation="MKIIAEGKGELIGWHDPDENRRWIRENKPRDLRDKRMSVKEAVA
                     QFVHDGDFIAFGGFGHIRIPMALVYEIVRQRKQNLVMAGKTAVHDIDILIGAGCVNKV
                     EVAYAFGHELRGLSPAGRHAVEAGQCKVVAEISNAAYQWRFLAGMMGIPFIPSRNMLG
                     TDTWTYSACKVVEDPWSGKPVCLIPAAYPDVAMFHVPRCDKYGNAQIEGIIVEDFELA
                     RAARRVIVTTEEVVDEEVIRAEPHRTVIPFYLVDALCEVPYGAHPTQMPYLYFFDEEH
                     IGEWFTLAETLEGIEAYFQKYVYGVCDFEEYLERVGGIRKLNYLKRVMQFKEPMRAPW
                     LKG"
     gene            3754..4533
                     /locus_tag="AC16V7_10410"
     CDS             3754..4533
                     /locus_tag="AC16V7_10410"
                     /inference="COORDINATES: protein motif:HMM:NF016764.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HIR6257573.1"
                     /translation="MAELEIAQTDTPGRFQFNWVPEVLFRPRRAFAKITAQNHGVWLT
                     PLLILTLMMVARVLASGWVRQNFAMTGELPLPPDFQYYSPEQQAQFMQAMQATQGPVF
                     IYVFPLISGVLGIWLGWLLVGGLLHLVTTMLGGRGDTMSAMNLVAWASLPFAVREGVR
                     VAALVISRQLIENPGLSGFAPIDSSGIMAYLSAWLPLVDLYLIWHIILLVLGGRAANG
                     LTPGKAMSGVVLTMLVILGLQALVSFLSTQLGSLTIIRPFF"
     gene            4558..5235
                     /locus_tag="AC16V7_10415"
     CDS             4558..5235
                     /locus_tag="AC16V7_10415"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6257574.1"
                     /translation="MEPFIRISGLRKTYKMGRVAVHALDGVDLDIPPNSFLVVMGPSG
                     SGKSTLLHLLGGLDRPTAGHIQVNGESLETLDENALAVFRRRTVGFIFQAFNLIPSMT
                     AQENVAFPMQFARVPRRQRKQRSMEVLKLVGLDDRASHRPTELSGGQQQRVAIARALI
                     NNPHLILADEPTGNLDTTSGASILQVLSGLHQSGRTVVVVTHDVRMTEAATHVVNLLD
                     GRIVDGA"
     gene            5269..7095
                     /locus_tag="AC16V7_10420"
     CDS             5269..7095
                     /locus_tag="AC16V7_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COG1361 S-layer family protein"
                     /protein_id="HIR6257575.1"
                     /translation="MSSIKNILTAKTLKTQSTQPMNKTSKIIQIGMPLLLVIALFGQT
                     TPALAQLAVSSVQPSTVVNITDVNLVITGAEFQGGASVVLQGLGALSTTFISSTVLNA
                     ELPAGASPGVYTVTVVNPDTTSASLDNAITILEPTATLEPATPTATQVASTTRPLIVV
                     DTYSASEDTIYAGQEFNLQIRLKNVGEDTALNLVATFTPGDFVPRKSGGVQAISEIDP
                     DEKKKITQALTASPDLTNKKIGALVMQVSYMDRDGTSYTETFNLSLPVISYGSGVAAT
                     STPTPTSPASNRPQLVITAYGTDVPVLQPGFRFQLHLQVNNLGNMDAKRVSMILGGGS
                     STGGSVDGTPEAGGISGASGDFGDFAPVDSSNVQFLGDMAVGAEFETHATLIVNAATE
                     PGAYPLKITFAYSDEKGKFYNDDQVVTLLVYTTPKVDVNFYRQPDPVFAGQPGLLPLQ
                     VVNLGRKSSVLGNMKVTAEGAQFSNNVILIGALDIGGYYTLDTTVTPDQPGPLTLLIT
                     IDYTDDFNQQQIISRTLTLNVEEMIMPESGPGGGGFEGGEFMPPPVQETFMQKLWRFI
                     KGLIGLDSGAPAPGPGEFPPGEFPPGEMPPPVESIPGSGPKG"
BASE COUNT         1708 a         1955 c         1789 g         1698 t
ORIGIN      
        1 caatatgctt tttataggca gcaaaatgca ctaaattacc cttaagtgta aaggtgggaa
       61 ttccatactt gatcgtctca tcagcatctg gtgcaacttc ccttatagtc atccttatct
      121 tttccaaaat ctctcgaact tcattaggga agcccgccat gtattcatcg atgtttgtgg
      181 ttgctgtttg atcagttctc atgttcttct tcatactacc cgaatacaac ctagaccgcc
      241 caatgggctg catgctgcgt tagcgagcgt ttttcgatct ggtttctacc aatgtagcca
      301 ttgatggcgc atctggtata tcacttgcct ttaaacttgc tactacgcta gcctgatttt
      361 ggggtggctg gttttgactc accttccact taccagataa gcgtgtgatg atcatttcaa
      421 caccaacaat agagctgatg agtctttcag tgaaatcttg aggagcatcg gaaaccgccc
      481 aaggctcaga gaatgatgct tcattgtgga tagtaagcgc ttctaattga gagcgcaacc
      541 aggatgcgtc gtctacaata cgtaaaatgc cataagcgtg tacaactgcg tagttccagg
      601 tgggtacaac cttgccagtt tccttctttg tcgcatacca agatggagaa atataagtgt
      661 cggggccgct gaacaccgcc aaggactcaa tatcactatt caaatcgctc cataatgggt
      721 ttgcacgagc aatatgacca cgcaaagttc catacggagg gggcgattcc gatagggaca
      781 aagggatgtg atttgcgttc aagccacccg aagacaatgt caccaaggta gccagtggat
      841 gagcgcggat tagttcgaac attacgtgaa attgcgtttc ttcaaattgt tttggtaaat
      901 acatgtttga ttcctatgtt cgtaacagca ccagactgag ccacctggcc agcctaaggc
      961 tagcatcgcg ccaacttctc cagcaagttg gtaggccttc ccgcttattg ttgttgcaaa
     1021 tacctagggc aaagtcaacg gtttccgact tcgtccacta tgggggctat tataaatggt
     1081 tgtacctgaa ggagtctatt tatctgacct aagtgcggtc agatcttcgt ctgacctaag
     1141 tgcggtcaga tcttcgacat ctcgacctac gggtaatcat cggaggctga tagacgagag
     1201 ggacgcctga aacatatatc tgtctctact tgcgaactat tacaccaata ggatccattt
     1261 cgtgaagcaa ccggagctct tcttccgtgg gtggatgagt gacctgaacc atttcgggga
     1321 tgaggatctc gaaagaagta ttggccctaa catcatccag cgtaactcca gggtgaagag
     1381 ccaagagttt aactcttttg gtttgctcgt caaagccata gagaccaagc tgggtgatta
     1441 ttcgataagg accagttcct tctggtagac ctgctcgctc cctagctcca ggaccatcta
     1501 agtaaccggg cgtcgtgata aagtctatct tctctacaaa gcggcgttta tcttgccgca
     1561 tgataataat agtgcgatga cagaaagaac ccaggtcatt cgctccgcca ctgccgggta
     1621 aacgcgtctt gggtcgttcc caatcaccga taacggtggt attgatgttg ccatacatat
     1681 caatcatcgc tgcgcctacg aaaccataat caacgtagcc cgcctgagag gcgctcatga
     1741 catcgtgcat gctggtagcg gcgacagctt tgtaaaaagt aaggatatcg cccacagaga
     1801 tgggcatttg ggacatccga gggccaattc ctccagcctc gaagataagc aacaaatttg
     1861 gcgcatgcgt cttctgagct aataaggcag caatcatggg taagccagtg cctaccaaaa
     1921 ccgatttctt gtcctctaga agcttggctg ccacacaagc taaaagctcg gtggaagtgt
     1981 acgctgctgt ggttactgtc atggatctcc tcctcacttc gcgcacttac gatacgattg
     2041 cgcttgaaga gtgtaaagaa gatgcaatcg ctcgggactt gcatttggac aaattaccta
     2101 actccaataa ctaacccttg agccaaggcg ctcgcattgg ctccttaaac tgcatcacgc
     2161 gcttgagata attgagcttc ctgataccac ctacccgctc aagatattct tcaaagtcgc
     2221 agacgccata gacatatttc tgaaagtaag cctcaatccc ctccaatgtc tcggcaaggg
     2281 tgaaccattc gccaatgtgt tcctcgtcga aaaagtagag gtagggcatc tgtgtgggat
     2341 gagcaccgta agggacctcg cacaatgcat ctaccaagta aaaggggatc accgtccggt
     2401 gaggctcggc gcggatcacc tcttcgtcaa cgacttcttc ggtagttacg atgacgcggc
     2461 gggctgcccg ggccaattca aaatcttcca cgataatacc ttctatctgg gcattcccgt
     2521 acttatcaca gcgaggcacg tggaacatag ctacgtcggg atacgcggct ggtatcaagc
     2581 aaacaggttt accgctccag gggtcttcca ccaccttgca cgcgctgtat gtccaagtat
     2641 cagtgcccaa catgttgcga gaggggatga atggaatccc catcatgcct gcgaggaagc
     2701 gccattgata cgcggcgttc gagatctcgg ctactacttt acactgacct gcctctaccg
     2761 catggcggcc cgccggcgaa agaccgcgca actcgtgacc aaaggcatag gccacctcaa
     2821 ctttattcac acagcctgcg ccgataagta tatctatatc gtggacagcg gtcttgccag
     2881 ccataaccag gttttgcttc cgttggcgga cgatctcgta aaccagagcc atcggaattc
     2941 ggatgtgacc aaagccacca aaagcaataa agtcgccgtc gtggacaaac tgcgccacgg
     3001 cttctttcac actcatacgc ttgtcccgaa gatcgcgcgg tttattctcc cgaatccatc
     3061 ttcggttctc gtcagggtca tgccatccaa tcagttcacc cttgccttct gcaataatct
     3121 tcatgattta tgcctccttg caatatcgtt cacacattgc cttaagagag gcctaggatt
     3181 ttacgagctt gaagtggttt ggcaatatct cgatttagct cctgggcaac acggactata
     3241 tgctccactg cttaaaaatc acgccggtag tttggtaggc ttgtgtgagc ataggagcac
     3301 actagcaatc catttggtct ttcaaattaa tttgtaacaa atttcacgat taaagtcaac
     3361 gatttgcggt ttcgctcccc tgcaaggaag tcaatcaatg gttgttccaa gacgggtctg
     3421 catgtaatag gcgcttgcta gttggcacgc gcgcccgaag ttgcttgtcc cgattattgg
     3481 gaacttttgc ttgcgaagca tcgtcataaa ggatggctaa tgcaaagccg cgtcattgca
     3541 agagcgctta aaccctcaca aatgacattt ttcgcgcgaa agaaaagcgc tggcgctgtg
     3601 gagttcctaa tgactggctt atatagactt tgcatcagcc ctacgtcaca gactagcata
     3661 atcattccga ggcttgtaga tatggtagaa tctgcactca attgtaagtt tggactagcc
     3721 caaaacgaaa atactggagc cattcgataa actatggccg aactagagat cgctcagaca
     3781 gatacgcccg ggcgctttca gtttaattgg gtacccgagg tattgttcag gccgcggcgc
     3841 gccttcgcca aaatcacggc tcaaaatcac ggggtatggc tcacgccgct gctcatcctg
     3901 accctgatga tggtggcgcg ggtgctggca tctggctggg tgcggcagaa cttcgccatg
     3961 accggcgagt tacccctccc accggacttc cagtactact ctccagaaca acaggcgcaa
     4021 ttcatgcagg cgatgcaggc cacccaaggc ccggttttca tctatgtctt ccctctgata
     4081 agcggggtgc tgggtatctg gctgggctgg ttgctggtgg gaggcttgct ccacctggtg
     4141 acaacgatgc tgggagggcg cggcgatacc atgtcagcca tgaacctggt cgcctgggcg
     4201 agtctaccct ttgctgtgcg cgaaggggtg cgtgtggcgg cgctggtcat cagccgccaa
     4261 ctgatcgaaa atcccggcct atccggtttc gctcctatcg attcatccgg catcatggcc
     4321 tacctttctg cctggttgcc gctggtcgat ctttatctca tctggcacat tatcctgctg
     4381 gtgcttggcg ggcgcgctgc caatggcctg acaccaggca aagcaatgag cggagtagtt
     4441 ttaaccatgc tggttatcct gggactccag gcactggtaa gtttcttatc cactcaattg
     4501 ggcagcttga ccatcatccg tcccttcttc tagaccacca tcgagtctaa aagcaatatg
     4561 gagcccttta tccgtatatc gggtctgcgc aagacctaca aaatggggcg ggtggctgtg
     4621 cacgccctgg atggagtcga cctggatatc ccacccaaca gctttctggt ggtgatgggc
     4681 ccttccggct cgggaaagag caccctgctg cacttgcttg gcgggctcga ccgcccaaca
     4741 gcgggacata tccaggtgaa cggggaatcc ctagaaactc tggacgagaa cgccctggca
     4801 gtcttccgcc ggcgaacagt aggctttatc ttccaggcgt ttaacctgat ccccagcatg
     4861 accgcccaag agaatgtagc cttcccgatg caatttgcgc gcgtcccccg ccgacagcgc
     4921 aagcaacgct cgatggaagt gcttaaactg gtcggtctgg atgaccgtgc cagtcatcgc
     4981 cccaccgagc tctccggcgg gcaacagcag cgcgtggcca tcgcccgcgc cctgatcaac
     5041 aacccgcatt taatcctggc tgacgagcca accggtaacc tggataccac gagcggcgcc
     5101 agtatcctgc aagtgctttc cggtttgcat cagagtggtc gcactgtggt agtggtcacc
     5161 cacgatgtgc gcatgaccga ggctgccact catgtggtca atctcttgga tggtcgcatt
     5221 gtggatggtg cgtaatgttg aaaacggatt ttgggaatgg atggtctcat gtcttcgata
     5281 aaaaatattc tcaccgccaa gacgctgaaa acgcagagta cccaacctat gaataaaaca
     5341 tctaaaatca ttcaaatcgg catgcccctt cttctggtca ttgctttgtt cgggcagacg
     5401 accccagcgc tagcacaact ggcagtttcc agcgtgcagc caagcacagt ggtcaacatc
     5461 acggatgtga acctggtgat aacaggcgct gagttccagg gcggcgcctc ggttgtgttg
     5521 cagggtcttg gggctttgag caccactttc atcagcagca cagtgctcaa cgccgagctg
     5581 cccgctgggg catcgccggg cgtttacacg gtcacggtgg tcaaccccga tacaacctca
     5641 gcctctttgg ataatgccat caccatcctg gagccaaccg ctaccctcga gccggccaca
     5701 ccaactgcca cacaggtcgc atctaccacc cgcccgctga ttgttgtcga cacctacagc
     5761 gccagtgaag acacaatata cgctggccag gagttcaacc tgcagattcg tctcaagaat
     5821 gttggcgaag ataccgcgct gaacctggta gccaccttca caccgggcga tttcgtcccc
     5881 cgtaagagcg gtggagtaca ggcgatcagc gagattgacc ccgacgaaaa gaagaagatc
     5941 acccaagcct taaccgccag ccccgatctg acaaacaaga agatcggcgc gctggtcatg
     6001 caggtctcct acatggatag agatggcaca agctacacag agacgttcaa tctcagcctg
     6061 ccggtcatct catacggtag tggagtggca gccacttcaa cacccacccc cacctcaccc
     6121 gcaagcaacc gcccgcagct cgtcatcacc gcttacggga cagacgtgcc tgtactgcag
     6181 cccggctttc gattccagct tcacctgcag gtgaacaatc taggcaacat ggatgccaag
     6241 cgcgtctcca tgatcttagg gggtgggagc agcacaggcg gtagcgtgga cggcacccca
     6301 gaagcaggcg gaatttctgg cgccagcgga gattttggcg acttcgcccc agtggattcg
     6361 tccaatgtgc agttcctggg agatatggct gtcggagcgg aattcgaaac acacgccact
     6421 ttaatcgtca atgccgccac cgagccaggg gcttatccct tgaagatcac ttttgcctac
     6481 agcgatgaga aaggcaagtt ctataacgat gatcaggtgg tcacgctcct ggtttatacc
     6541 actcccaagg tagatgttaa tttctaccgc cagcctgacc cggtcttcgc cggccaaccc
     6601 ggcttgctgc ccctgcaggt ggtgaacctg gggcgcaagt cgtctgtgct gggcaacatg
     6661 aaagtgactg ccgagggcgc acaattcagc aacaacgtca tcctgatcgg ggcattggat
     6721 atcggtgggt actacacgct agacaccaca gtcaccccag accagcctgg tcctttgacc
     6781 ctgctgatca ccatcgacta caccgatgac ttcaaccagc agcaaatcat ttcgcgcacc
     6841 ctgactttga atgtcgagga aatgatcatg cccgaatcag gtcccggtgg aggcggcttt
     6901 gaaggcggcg agttcatgcc tccacctgtt caagaaacct tcatgcaaaa actctggcgc
     6961 ttcattaaag gactgattgg cctggacagc ggcgctcctg caccgggacc aggcgaattc
     7021 ccacccggcg agttccctcc gggcgagatg ccaccacccg ttgaatccat tccaggctca
     7081 ggaccaaaag ggtaatctgt ttaagattgg tgattgaaga tttttgattt tggattgtcg
     7141 ttagctaatc
//
LOCUS       DBNHOH010000595         3257 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1766524, whole genome shotgun sequence.
ACCESSION   DBNHOH010000595 DBNHOH010000000
VERSION     DBNHOH010000595.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1766524"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..2680)
                     /locus_tag="AC16V7_10425"
     CDS             complement(194..2680)
                     /locus_tag="AC16V7_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIR6257576.1"
                     /translation="MRFDRFTERAQEAAQRAAEIIQRYGHNQIDTEHILLALIEQPQG
                     VIPQILEFLKVDAAALIERLDYILRTSPKANIFGGGAGQIFITPRVKRIIDLANEEAN
                     RLKDEYISTEHIFLAILSERSTPAARLLEGAGVTRERVYDTVQQIRGGQRVTDPQAET
                     RYRTLEKYSRDLTQSAREGKLDPVIGRDAEILRVIQILSRRTKNNPVLIGEAGVGKTA
                     IVEGLAQKIASNDVPEILTGKRVVALDLGAMIAGSRFRGEFEERLKVAIEEVQRAAGE
                     VILFIDELHTVVGAGAAQGAMDASNMLKPALARGELQCVGATTLDEYHKHIEKDAALE
                     RRFAPVFVEEPNVEDTIQMLRGLRDRYEAHHKVRFSDDALVAAARLSARYVTDRHLPD
                     KAIDLMDESAAKLRVALYSLPPEIKDLKVEIDRLAAEEEQSGLERDYERAAQKKAERL
                     RLETEFNEKRDQWEAEHQLDEVVDVNDIAEVVAQWTGIPLHQMMETEAEKLLQMEVRL
                     HERIKGQDEAIKAISDAIRRARSGLKDPRRPIGSFIFIGPSGVGKTELARALARFMFE
                     DEDALVRIDMSEYREQHTVSRLFGAPPGYVGYEEGGQLTEAVRRRPYRVILFDEIEKA
                     HPEVWNALLQILDDGRLTDGQGHIVDFRNTVLIMTSNLGTEFVRKAGSLGFLQRKDDD
                     EERAAHEKIEKALKSTFRPEFLNRIDEIITFSPLSLEQMREIVDLQMNEVQERLSEHG
                     LVVELSEEARVWLANVGYDPSFGARPLRRALQKHVESPLSVSLLSGEFSSGDKILVEV
                     DEEKNELTFCRVEQASDQSIPAEEVSQVEA"
     gene            complement(2889..>3257)
                     /locus_tag="AC16V7_10430"
     CDS             complement(2889..>3257)
                     /locus_tag="AC16V7_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR6257577.1"
                     /translation="ATYTPSPTSSPTATITPTATPVPTDTPTPGLALGASASLPPFRL
                     RQSPEGPAIGPALRTGSPLTVLYGVQIVDGLVWVEVQDSEGRVGWVPQIYLKLITPTP
                     TRTPSLTPTALTPTSSPTPP"
BASE COUNT          629 a          910 c          948 g          770 t
ORIGIN      
        1 ttcctgaatc tgtggataaa catcattaaa agtaatgatg tttcttggac aatcgtcata
       61 atcacttgca aattttgctc atacggagat gagacagggg gtgaaggagt tgcaaaaaga
      121 ggggttctgc aggataaata ggatcctgca gaacccctca tgttgtttca tccaaggttg
      181 agaagaattc ttatcaggcc tcgacctggc tgacctcttc tgctggtatg ctttggtcgc
      241 ttgcctgttc cacgcggcag aaggtaagct cgttcttctc ctcgtccact tccaccaaga
      301 tcttatctcc tgacgagaac tcgccactga gcaggctgac cgaaagcggg ctttccacgt
      361 gcttctgcaa ggctcggcgt agtgggcggg caccaaaaga tggatcgtag cccacattgg
      421 ccagccagac acgggcttct tcgctcagct ctaccaccaa gccatgctcg ctcaggcgct
      481 cctgcacctc gttcatctgc aagtctacga tttcgcgcat ctgctccagg ctgagcggcg
      541 agaaagtgat aatctcgtcg attcggttca ggaactcggg gcggaatgtg ctcttgagtg
      601 ccttttcgat cttctcgtgg gcggcacgct cttcatcgtc gtctttgcgt tgcaggaagc
      661 ctaagctgcc cgctttgcgc acaaactcag tgcccaggtt gctggtcatg atcaaaaccg
      721 tgttgcggaa gtccacaata tgaccctgcc catcagtcag gcggccgtca tccaggatct
      781 gcagcaacgc attccatact tcaggatggg ctttctcgat ctcatcgaac aggatgacgc
      841 ggtatggccg gcggcgtact gcctcggtga gctggcctcc ttcctcgtac ccaacatatc
      901 caggaggcgc accaaacagg cgggagacag tgtgctgctc tcggtattcg ctcatgtcga
      961 tgcgcaccag ggcgtcttcg tcttcgaaca tgaatcttgc cagcgctcta gccagctctg
     1021 tcttgccgac ccctgaaggg ccgatgaaga tgaacgagcc gattggcctg cgcgggtcct
     1081 tcagaccgga gcgggcgcgg cggatggcat ccgagatagc cttgatggct tcgtcttgcc
     1141 ctttgatgcg ttcgtgcagg cgaacctcca tctgtagcag tttctccgcc tcggtttcca
     1201 tcatctggtg caaagggatg cctgtccatt gtgcgacaac ctctgcgatg tcattgacat
     1261 ccaccacctc gtcaagctgg tgctcggctt cccactggtc gcgcttttca ttaaattcag
     1321 tttccaatcg caggcgctct gcctttttct gcgccgcgcg ttcgtaatcg cgctccagtc
     1381 cagattgttc ctcttcggca gccaagcgat cgatctcgac ctttaaatcc ttgatctcgg
     1441 gtggcaggga gtagagcgcc acgcgcaatt tggccgccga ttcgtccatt aggtcgatgg
     1501 ctttatccgg cagatgccgg tcggtgacgt agcgggcaga gagccgcgcc gcggctacca
     1561 gggcatcgtc ggagaaacgt accttgtggt gtgcttcgta gcggtcgcgc agcccgcgca
     1621 gcatttggat ggtgtcttcc acgttgggct cttccacaaa gaccggggca aagcggcgct
     1681 ccagggcggc gtccttttcg atgtgcttat ggtactcgtc cagcgtggtg gcgccaacgc
     1741 actgcagctc accgcgtgcc agggctggtt tgagcatgtt ggaggcatcc atagcgcctt
     1801 gtgcggcgcc tgcgcccacc actgtatgca gctcgtcgat gaacaagata acctcaccgg
     1861 cggcgcgttg cacttcttca atcgcaactt tcaagcgctc ttcaaattcc ccgcggaagc
     1921 gggagccggc aatcatcgcg cccaggtcga gggcgaccac gcgtttgccg gtgagaatct
     1981 ccggcacgtc attgctggcg attttctggg ctaagccttc tacaatggcg gtcttgccaa
     2041 ccccagcctc gccgatcagc acggggttat tcttggtgcg gcgggagaga atctggatca
     2101 cgcgcagaat ttcagcatcc ctcccaatga ccgggtctag cttgccctca cgggcagact
     2161 gggtcaggtc gcgcgagtat ttttccagtg ttcggtagcg agtctcagct tgcggatcgg
     2221 tgacacgctg cccgccacgg atttgctgaa cagtatcgta gacgcgctcg cgggtcacgc
     2281 cggccccttc aagcagtcga gcggctggcg tgctgcgctc gctcaggatc gctagaaaga
     2341 tgtgctcggt agagatgtac tcgtctttga gacgattggc ttcctcgttc gccaggtcga
     2401 tgatgcgctt cacgcgtggg gttatgaaga tctgcccggc cccaccgccg aaaatattgg
     2461 ctttggggct ggtgcgcaat atgtaatcta accgctcgat cagggcggcg gcatctactt
     2521 tgagaaactc caaaatttgc ggaatcacac cctgcggctg ctcgataagt gccagcagga
     2581 tatgttctgt gtcaatttgg ttgtggccgt accgctggat gatctctgcg gcacgctgag
     2641 cggcctcttg ggcccgctcg gtaaaccgat caaatctcat catgggctat tttccttcct
     2701 ttggagaaac gcaaactcag cttgtttgag tttatctcca cactgattat aacattctga
     2761 gggtgtggaa gaattggcgg cgcataagag ttgtatatga atttcgaaaa aatgttcgca
     2821 taaagatggc tttcgcatct caggacagtt atcccctgcg tgctcatctc tcactgcgta
     2881 tgtgagattc atggaggggt aggtgacgaa gtgggtgtca aagcagttgg agtcagcgaa
     2941 ggcgttctgg ttggggtggg ggtgatcagt ttcaggtaaa tctgaggcac ccagccaact
     3001 cgcccctcgg aatcctgtac ctcgacccat accaggccat cgacgatttg aaccccgtag
     3061 agcacggtga gcggcgaccc tgtgcgcagg gctggcccaa tcgccggccc ctcaggcgat
     3121 tgccgcaagc gaaaaggggg gagtgaggca ctggcgccca gtgccaagcc cggagtgggc
     3181 gtatccgtgg gcacgggggt tgcggtgggg gtgatagtcg ccgtgggcga actggtgggc
     3241 gagggggtat aggtggc
//
LOCUS       DBNHOH010000596         2684 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_163282, whole genome shotgun sequence.
ACCESSION   DBNHOH010000596 DBNHOH010000000
VERSION     DBNHOH010000596.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_163282"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="AC16V7_10435"
     CDS             <1..1114
                     /locus_tag="AC16V7_10435"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR6257578.1"
                     /translation="KMRSALYKVWLNRDFTEYSQLTAQDMSLPNWQPSNKFRLYVRKD
                     VVASLWNYGAAPAEEIVLADPYEGKEIKLSADRAVGTLGTEPGQFKRPRGIAVGPDDS
                     LYVADTENHRIQRFAPDGTFINAWGSFAASTEGAPAAEGLFNEPWGIAVGPDGAVYVA
                     DTWNHRIQKFSAEGEFIKTWGFGISQTDDPLGFYGPRGIAVDNQGHVFVTDTGNKRVV
                     VFDTDGNYVTKFGSTGLGLGQFDEPVGIAVNSAGLVYVADTWNQRIQTFIAEVNGSYL
                     PVNSWEISGWYGQSLDNKPYIAVDNQGQIFVADPEGYRVLQFSSEGEFIQYWGDFSIE
                     LDGFDLPSAVAADTEGGIWVSDTNNNRILHFTLPPK"
     gene            1115..2251
                     /locus_tag="AC16V7_10440"
     CDS             1115..2251
                     /locus_tag="AC16V7_10440"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6257579.1"
                     /translation="MAISIHPVISLFDQHSFYNFAWRVFRDDPNWVPHLWPQRKAYLT
                     RKAAFFNYGEGEFWLAKRGREVVGTIGTAIDHSRNRHKGQQTAIFGFFESLPGDYAIA
                     QALWDHACGWAKSRAMITLQGPYNFAANEDPGFLVEGYNCPPCIMMGHTPPDYAIYAQ
                     RYGFEKAQESLAYRYDLAQINFDVANAPEVVRRIADRTTKHHGKEVIRNPLLADWDIE
                     IERLHPVYNTSLAVLPEFSPIELVEFRAQAASLKPILDPELVFIAEVDGKSVGFALGL
                     PNINQALIHANGLRYPWDYLRYTLAQRKIHSASFKILAIDPAYWGHGLEALMFIEMAK
                     AIIRKGYTWVDASLTNEMNPQTNKLATRLGAKVYRRYREYQIPL"
     gene            2532..>2684
                     /locus_tag="AC16V7_10445"
     CDS             2532..>2684
                     /locus_tag="AC16V7_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR6257580.1"
                     /translation="MKLHEYQSKQIFSRYGIPIPKGRIAATAQEAEHIAEELGGRVVI
                     KSQVLVG"
BASE COUNT          701 a          633 c          681 g          669 t
ORIGIN      
        1 gaagatgcgt tctgcactat acaaagtgtg gctaaaccgc gattttaccg agtatagcca
       61 attaactgca caagatatga gcttgcctaa ttggcaaccc tctaacaaat tcaggcttta
      121 tgtacgcaag gatgtcgtcg ccagtttatg gaactatgga gctgccccag ctgaagaaat
      181 cgtgcttgct gatccttatg aaggcaaaga gataaagcta agtgcagatc gagcggtcgg
      241 cacactgggg actgaaccag gacagtttaa acgaccacgt ggcattgctg ttggtccaga
      301 tgatagcctg tatgtggcag atactgaaaa tcaccgcatc caacgctttg cacctgacgg
      361 taccttcata aatgcctggg ggagttttgc agctagcaca gaaggcgcac ctgcggcgga
      421 gggcttgttt aacgagccct ggggaattgc cgttggtcca gatggagcgg tatatgttgc
      481 tgacacctgg aatcacagga ttcaaaaatt cagcgcagaa ggcgaattca tcaagacttg
      541 ggggtttggt atcagccaga cagatgatcc cttaggattt tatggaccac gtgggattgc
      601 tgtggacaat cagggccatg tctttgtcac agacactggc aataagcgcg tggtcgtttt
      661 cgacactgat ggtaattacg taaccaaatt tggaagtaca ggtttaggcc ttggtcaatt
      721 cgacgagccg gtgggcattg cggtaaatag cgccggccta gtatacgtgg cggacacatg
      781 gaaccagcgc attcaaacct tcatcgctga ggtgaatgga agctacctgc cggtcaatag
      841 ttgggagatt agcggttggt acgggcaatc tttggataat aagccttata tcgcggtgga
      901 caaccagggg cagatctttg ttgccgaccc agaaggctat cgggtattac agttttcatc
      961 cgaaggtgag tttatccaat attggggcga tttctccatc gaattggatg gctttgactt
     1021 accatcagct gtggctgccg acaccgaggg tggcatctgg gtaagtgaca ccaacaataa
     1081 caggatattg cactttaccc tgccgccaaa atagatggcg atttcaatcc atccagtcat
     1141 aagcctattt gaccagcatt ctttttacaa ttttgcctgg cgcgtcttta gagacgaccc
     1201 caactgggtt cctcatttat ggcctcaacg taaggcctac ctgacccgta aagctgcttt
     1261 cttcaattat ggcgaagggg agttttggtt ggcaaagcgc gggcgagaag tcgtcggcac
     1321 cattggaact gccatagacc actctcgcaa tcgccataag ggacaacaaa cggcgatttt
     1381 tggctttttt gaatccttgc ccggtgacta cgcaatcgca caggcattgt gggatcatgc
     1441 ctgtgggtgg gctaaatcac gggcgatgat cacgctccag ggtccataca atttcgcagc
     1501 caatgaagat cctggctttc tggtggaagg ctataattgc ccgccctgta tcatgatggg
     1561 acatacccca cccgattatg ccatttatgc tcagcgctat ggttttgaga aagctcagga
     1621 aagcctggct taccgctatg atttagccca aattaacttt gatgtagcca atgccccaga
     1681 ggttgttcgc cgcatagctg atcgcacaac gaagcatcac gggaaagagg tgataagaaa
     1741 tccgctgtta gcagattggg atatagaaat tgaacgcctg cacccggttt acaacacttc
     1801 cctggcagtt ctgcctgagt tttcacccat cgaattggtt gaatttcgcg cccaggctgc
     1861 ttcgcttaag ccaatcttag accctgagct agtcttcatc gcagaagtgg acggtaaatc
     1921 ggtaggtttc gccctaggat tacccaatat caaccaggca ctcatccatg ccaacggatt
     1981 gcgttatccc tgggattacc ttaggtacac actcgcccag cgaaaaattc acagtgccag
     2041 cttcaaaatc ttggctattg acccagcata ttggggtcac ggattggagg cgcttatgtt
     2101 tatcgagatg gcaaaggcga ttatacgcaa aggttatacc tgggtcgacg cttccttgac
     2161 caacgagatg aatccacaaa ccaacaaact agctacccga ttgggagcca aggtatatcg
     2221 ccgctatcgc gagtatcaaa ttccattgtg aaccgaatgg atatctttct tctgctaggt
     2281 tgttggggga aattcttcag atcagcaggt caattacata tgcccggaag ggcatgcgca
     2341 atctcgcaaa aagagtgttt ggtgtgtatt ggtgggggta tgccaccacc aaagcaccca
     2401 ttttctctcc tcagtggtgg gaaatcgccc aaatgcgatt gccctatcag attcgctttg
     2461 cctcaacagt ccaaatggta taatgccttc gctatcatgg tcataagcca aacgcaggga
     2521 gagcaatttg catgaaacta catgagtacc aatcaaaaca gatcttttcc aggtatggta
     2581 tcccaatccc gaaaggtaga atagcggcaa ccgctcaaga agcagagcat attgcagaag
     2641 agttaggagg ccgcgttgtc attaaatccc aggtgctagt cgga
//
LOCUS       DBNHOH010000597         1717 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1317837, whole genome shotgun sequence.
ACCESSION   DBNHOH010000597 DBNHOH010000000
VERSION     DBNHOH010000597.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1317837"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="AC16V7_10450"
     CDS             <1..814
                     /locus_tag="AC16V7_10450"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HIR6257581.1"
                     /translation="ATELGDWGFMPTEPEALRKEIESRKLVMLGAYIHVAFKYPASVV
                     SRREEVLKTARLLAACSPNHKPYLILADDNAADPVRSAVAGRAPADVGLSASEWRLFA
                     RGVEETAKAVQDETGLPSLFHHHCAGYVETPAEIDSLLENTDSKLVNLVFDTGHYVFG
                     AGADGPGADGDLSSVLDHYGERIAYVHFKDCEPRIAAQSREEQWEYLKSLRHGVFCEL
                     GQGCVDFKGVYSWLQKRNYYGWVLVEQDVLPGMGSPKDSAQRNRDYLKSIGL"
     gene            954..>1717
                     /locus_tag="AC16V7_10455"
     CDS             954..>1717
                     /locus_tag="AC16V7_10455"
                     /inference="COORDINATES: protein motif:HMM:NF012742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR6257582.1"
                     /translation="MSRALNDNPLISQEVRTRVQALAESMGYSPNALAQSLQSRLSNS
                     VGLVITTIADPFFSELTLGLEEVAHRADISVFLASSHNDPEREIKIIQTFERRRVDGV
                     IVASSRIGSEYASRLEQIRIPVVMINSQASGDYENLYSVAVDDYQGAQLAMQHLLDMG
                     HRRIGYIGVQNRPGSNERRMRGYLDALKACGIAAQPGWVLLGSPQATEDFAGDVEVGQ
                     VLMSELVQENVTAVFCYCDTVAAGVMIACRKLGLSIP"
BASE COUNT          350 a          446 c          522 g          399 t
ORIGIN      
        1 ggccaccgag ctgggggatt gggggttcat gcccaccgaa cctgaagcgc tacgcaagga
       61 gatcgaaagc cgcaagttgg tcatgctggg cgcatatatt cacgtggcgt tcaaatatcc
      121 ggcttcagtt gtctcacggc gggaagaggt gttgaaaact gcacgcctgc tggccgcttg
      181 ttccccaaac cataaaccct atctcatctt ggcagacgac aatgccgctg acccagtacg
      241 ctctgccgtt gctggcaggg ctcctgcgga cgtgggttta agcgctagcg aatggcgtct
      301 cttcgcccgg ggggttgagg agactgccaa ggctgtgcag gatgagaccg gcttgccgag
      361 cttgttccac catcactgtg caggttatgt agaaacccct gctgaaatag actcgctatt
      421 ggagaacacc gactcgaagc tggttaatct ggtgttcgat accggtcatt acgtgttcgg
      481 cgcgggggct gatgggccgg gcgcggacgg tgatctctcc tcggtcctgg atcactacgg
      541 cgagcgcatc gcttatgtcc actttaaaga ctgcgagccc aggatcgcag cccaatctcg
      601 tgaagaacaa tgggaatacc tgaaatcact gcgccacggt gtattttgtg agctgggtca
      661 gggttgtgtg gacttcaagg gtgtctactc gtggctgcag aagcgcaatt actacggttg
      721 ggtgctggtc gaacaggatg tcctgcctgg gatgggttct ccaaaagata gcgcccagcg
      781 aaatcgtgac tatctcaagt cgatcggttt gtaactactg accgagccgg gctggttcgc
      841 tccgcagtgc tatgccgttc gaaccagccc gaaagtaaat gctgatcatg ccgcgtgcgc
      901 gaagagtctc catcaaggat atcgctaaag ccgctggagt gtcttattcg accgtgtcca
      961 gggctttaaa cgacaaccct ctcatcagcc aagaggtgcg cactcgcgtt caagctttgg
     1021 cggagtcgat ggggtattct cccaatgccc tggctcaaag ccttcaatcg cgcttgtcaa
     1081 actcggttgg attggtgatc actaccatcg cagacccttt cttctctgag ctgacgctcg
     1141 gcttggagga ggtcgctcat cgggcagata tcagcgtctt cctggcctcc tcgcataacg
     1201 acccggagcg ggagatcaag atcatccaaa cctttgagcg tcggcgtgtg gatggtgtta
     1261 tcgtcgcctc gtcccgcatc ggtagcgaat acgccagccg gctggagcag attcgtattc
     1321 cggtggtgat gatcaacagt caggctagcg gggactatga gaatctgtat tcggttgcag
     1381 tggacgatta tcagggagcg cagctggcaa tgcagcacct gctggatatg ggtcaccggc
     1441 gcattggtta tattggcgtc caaaaccgcc ctggttcgaa tgagcggcgg atgcgcggct
     1501 accttgatgc cctaaaggca tgtggtattg cggctcagcc cggctgggtg cttctgggat
     1561 cgcctcaggc gaccgaggac tttgccggag atgttgaagt gggtcaggtt ttgatgtcgg
     1621 aactggttca ggaaaatgtc acggcggtgt tctgctactg cgatacagtg gctgcaggcg
     1681 tcatgatagc ctgtcgtaaa ttggggctca gcatacc
//
LOCUS       DBNHOH010000598         3984 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_420093, whole genome shotgun sequence.
ACCESSION   DBNHOH010000598 DBNHOH010000000
VERSION     DBNHOH010000598.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_420093"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="AC16V7_10460"
     CDS             <1..384
                     /locus_tag="AC16V7_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR6257583.1"
                     /translation="GLTLETAILFLPGLAYLLFAETQGSGSFAHAGWQVTLLLALTGV
                     VTALPLLLFASAARSIPLYMVGILQFIAPTCQFLLGVFLYREPFTQIQLVGFSLIWSA
                     LLFYWLEGVLERRKLILRQTQIKTS"
     gene            complement(647..955)
                     /locus_tag="AC16V7_10465"
     CDS             complement(647..955)
                     /locus_tag="AC16V7_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257584.1"
                     /translation="MRQKQPPKPQKKMDQRQQVQQELERQQEQSQKLRQEEQQELEQQ
                     QEQRRKLRQEEQQELERQQEQKRKLRRQEQQKLEQQQEQERKLRQKEQQKLEQQQRRK
                     "
     gene            complement(1213..1695)
                     /locus_tag="AC16V7_10470"
     CDS             complement(1213..1695)
                     /locus_tag="AC16V7_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009780434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HIR6257585.1"
                     /translation="MDPVNGYSLELVIGDYEERFCPMTELKTQKNDASVEDFLNAVPD
                     EKKRRDCFAVLELMGDITGERPAMWGTSIVGFGSYHYKYASGREGDWFLTGFSPRVQN
                     LTLYIMSGFDNYEALLADLGKYKTGKSCLYINKLEDIDLRTLRKLIRQSVAHVAETNK
                     "
     gene            complement(1893..3206)
                     /locus_tag="AC16V7_10475"
     CDS             complement(1893..3206)
                     /locus_tag="AC16V7_10475"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HIR6257586.1"
                     /translation="MSQYDFEKFFELSLDMLCIAGTDGYFKRVNPSFQRVLGWTPEEL
                     TGRPFIDFVHPDDIEATLKEVEKLASRIPTISFRNRYQCSNGYYRHLLWTAFPEKETG
                     LIFAIAHDTTELVEANQRFQLAVDASPVSQIMVDQHGLIQLINRETERLFGYSRDLLI
                     GKSIEMLVPATIDEQHQRDRSAFFQNPGTRLMGEGRHVRAMAQDGREFPAEIGLNPVQ
                     FSDGVYVLCTVIDLTFQKKSEERMMQLAKELEEANAQLSQLAMTDRLTSVFNRHAFDE
                     QLDHLTQLMQRMGKPISLLMIDIDHFKRYNDRYGHPMGDEALKTVASLLCQNARTSDV
                     VARYGGEEFAIIMPNTAEEEAMQLSDRLRKIFHAHEWEKEMLTISIGASTIFHGKTVD
                     ETKSKKSKYDARLIIDADQALYYSKNNGRDRATHVSEIPDTDHQT"
     gene            complement(3771..3947)
                     /locus_tag="AC16V7_10480"
     CDS             complement(3771..3947)
                     /locus_tag="AC16V7_10480"
                     /inference="COORDINATES: protein motif:HMM:NF018096.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HIR6257587.1"
                     /translation="MTKVVHCRDLGFDCEGVVRAESAEEALQMVAQHAKEVHGMEEVP
                     AEVVEKVHEVMQEE"
BASE COUNT          914 a         1064 c          876 g         1130 t
ORIGIN      
        1 ggcttgacgt tggaaacagc tatactcttc ctgcccggcc tggcttacct cctatttgcg
       61 gaaacccaag gcagtggatc cttcgcccac gctggctggc aggtgacgct cttactggcg
      121 ctgaccgggg tcgtcactgc gctgccgctg ctcttgttcg cctcggcagc ccgatcgatt
      181 ccgctctaca tggtgggcat cttgcagttc atcgccccta cctgccagtt cctgctgggt
      241 gtgtttctct accgcgagcc atttacacaa atccaattgg tgggattcag cctgatttgg
      301 agcgctctct tgttttactg gctggagggg gttttagagc gtcgcaagct gatcttgaga
      361 caaacacaaa taaaaacctc ataaataaaa gaaatccgcc gccaacccga aatggattca
      421 accatggaaa caggtcagcc ccaaagcgct gccccaagcc ttcgccagcc taaatttcct
      481 tattaacgaa tacccaatac ctgcctgcgc aggcaggtat tggacgcgca accaccccac
      541 ggtgtgccct acactatgta accctctaag atctctaact ctacgattat cgcgtccagc
      601 ctgggcatca gacgagaaag gcgcagggac tccgcagcaa cttttgttat ttccgtctct
      661 gttgctgctc tagtttttgc tgttcctttt gccgcagttt ccgttcctgt tcctgttgct
      721 gctccagttt ttgctgttcc tgtcgccgca attttcgttt ctgttcctgt tgtcgctcca
      781 gttcttgctg ctcttcctgc cgcaattttc gtctctgttc ctgttgctgc tcaagctctt
      841 gctgctcttc ttgccgcagt ttttgtgact gttcctgttg tcgctccagt tcttgctgca
      901 cttgctgccg ttggtccatt ttcttctgcg gcttcggcgg ttgtttttgt ctcattttgt
      961 tttccttctt gagcgctaat cttacctgca cgccacacag actcacagct ggtgttggcg
     1021 acccaaccat ctttctgtgg ggctcttata ttgtaatcta actgcatggc tatgcgattc
     1081 gttcttgtgc ctttggtcat gagcttcccg tttggtctga agaccagttc aggccaggga
     1141 actaccgggg ctagtcccgt ctacaatgag cgctgcattc ctgctcccgc agatggtcag
     1201 gcacgacctg cgctatttat ttgtttcagc aacgtgggcc accgactgcc tgatcagctt
     1261 tcgcaaggtg cggagatcaa tatcttccag tttgttgata tacaggcagg atttgcctgt
     1321 cttgtacttg ccaagatccg ccagcaacgc ctcgtagttg tcaaagcccg acataatata
     1381 gagggtcaag ttctgcaccc tgggcgagaa accggtcaga aaccaatctc cttcacgccc
     1441 ggaggcgtat ttgtaatggt agctcccaaa tccgacgatg ctcgttcccc acatcgccgg
     1501 ccgctcccct gtgatatcgc ccatgagctc taagacagcg aaacaatcac ggcgcttctt
     1561 ttcatcgggc acagcattga gaaagtcttc aacgctggcg tcattcttct gcgtctttag
     1621 ttcggtcatt ggacaaaacc tttcttcata atcaccaatc accaactcta aggagtaacc
     1681 attgacagga tccacgtatc catccatgca tcagacagag agttgtcaac ggtggcggga
     1741 aataccaaat tgctcctaat accgactaag caaaggtctt cataagccaa aaggtaatgg
     1801 acttcatcaa ttagtcaatg gatactgtca aaccgccatt tttttctacg tgaccggagc
     1861 atagtgaaag caaacacgct tggaagcagc tgttaggttt gatgatctgt gtctggtatt
     1921 tcagaaacgt gggtagcacg atctctacca ttattctttg aataatagag agcttgatct
     1981 gcgtctatga taagccgcgc gtcgtatttg ctctttttac tcttggtctc gtctactgtc
     2041 ttaccatgga atatcgtgga agcaccgatg ctgatcgtca gcatttcttt ctcccactca
     2101 tgagcatgaa agatcttccg cagcctatca gaaagctgca tggcctcttc ctcggctgtg
     2161 ttgggcatta taattgcgaa ctcttctcct ccatacctcg ctacgacatc actggtgcgc
     2221 gcattctgac acaacagact ggctacggtc ttcaaagcct catcgcccat tggatgtcca
     2281 tatcgatcat tatatcgttt gaaatggtcg atatcgatca tcaagaggga gatgggttta
     2341 cccatccgct gcatgagttg ggtgagatgg tcaagttgct catcaaatgc gtgtcgattg
     2401 aacacgctgg tcagcctgtc agtcattgcc agctgtgaca gttgtgcatt tgcctcctca
     2461 agttcttttg ctaattgcat cattctttct tccgactttt tttgaaatgt cagatcaatg
     2521 accgtgcaga gaacgtatac tccatcacta aattgaacgg ggttgagtcc aatctcggct
     2581 ggaaattctc tcccatcttg agccatcgcc cgaacatgcc ggccctcacc catcaaacgc
     2641 gtaccagggt tctgaaaaaa agcggatcga tcccgctgat gctgctcatc aatagtggct
     2701 gggaccagca tttcaatcga tttcccgatc aagaggtccc gtgaataacc aaagagacgt
     2761 tccgtttccc tgtttatgag ttgtatgagg ccatgctgat caaccatgat ctgggataca
     2821 ggcgaagcgt caactgccag ttggaaacgc tgattcgcct cgaccaactc agtcgtgtca
     2881 tgtgcaatcg caaaaatcaa tcccgtttct ttttccggaa aagccgtcca caggagatgt
     2941 cggtaatacc catttgaaca ttgatagcga tttcgaaatg atatagtggg gatccgggat
     3001 gccagctttt ccacttcctt caatgttgcc tcaatgtcgt cgggatggac aaagtcaata
     3061 aaaggccggc cggttaattc ttcaggtgtc cagccaagaa cgcgttgaaa tgaagggttg
     3121 acgcgtttga aatagccatc ggtgccagca atgcaaagca tatccagtga aagctcgaag
     3181 aacttttcaa agtcatattg agacattgaa tttctccttg atctttgcct gatgaaatat
     3241 taatggcaac caccgacaaa attcatcagc ttgccaccac tccatcatac cgagagtgaa
     3301 aggtcctatt aatgctttac agtatttcat acctgattaa ctaccttagc gaattcgttg
     3361 acaattttat tagcaagagg gatgagcgta tacaagcagt tcagatctaa tcctaccaga
     3421 taatttcaat gctgctacaa tactatctga ttaggcatat tgtgagtgtc agccacttta
     3481 tggagcaatg tttaaaccaa cgagagttgg gcagcatccc cacagtacac ctaattatcg
     3541 cttatactct cctccaccgc attcggaggg atccgagacg gaaaatagct taagcctcta
     3601 cgatttcctc atgccagcca tgccttcaat atgaaccggt aaacatagta cagccgcgcg
     3661 attcctgcgc ccgttccatt gagtatattg tcagcatgta tgcactgcgg acgtaaatca
     3721 cttatgaaat tgtaggcgtc gatggagtgg cgtaatgttt agtgggtggc ttactcttcc
     3781 tgcatgacct cgtggacttt ctccacgacc tcagcgggaa cctcttccat gccatgcacc
     3841 tctttggcat gttgggcaac catctgcagg gcttcctctg cgctctcagc tcgcactacg
     3901 ccctcgcagt caaaccccag atccctacaa tgcacaactt tcgtcatttt tagcctcctt
     3961 ttgacatacg ctacatattt tatt
//
LOCUS       DBNHOH010000599         2594 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1638096, whole genome shotgun sequence.
ACCESSION   DBNHOH010000599 DBNHOH010000000
VERSION     DBNHOH010000599.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1638096"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="AC16V7_10485"
     CDS             <1..872
                     /locus_tag="AC16V7_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257588.1"
                     /translation="TRLLDYRLSTAEERLVHDFAVDFPGQDLAAVWTRYEGSPSFDGR
                     YWGFMAEDQEWLATALLVYDLKIDRVVARRDLPSRPEIDSVSISPLGGFFLAYYDGYC
                     EPGQLGSDAHPCGLMVYDRDLQNGRGLLRIVGHSDLALDANQREVLIFQDIDTDNISL
                     LGLESGEVTPLWPIDFSHSALGLHFSGRASRMPGWALVSTYNGAQPAATWMDDQIFVV
                     ELKPGGRVVRLVHTYSIVDENQEHDYWAEPHASVNHDFTRIVFTSNWGRSGTGEVETY
                     LIELPPDWLSQLP"
     gene            complement(959..1249)
                     /locus_tag="AC16V7_10490"
     CDS             complement(959..1249)
                     /locus_tag="AC16V7_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257589.1"
                     /translation="MIIVRNVFQAKYGKGDELVQLMKDSQAIWPSERKVRIMTDLSGP
                     FFTVVTEADYESFSAWEADAKKTFGDDRFAGLFERMMPLVESGSREFYTLVE"
     gene            complement(1359..>2594)
                     /locus_tag="AC16V7_10495"
     CDS             complement(1359..>2594)
                     /locus_tag="AC16V7_10495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR6257590.1"
                     /translation="VLLVFGLVFFHTARIFDTISLPEGVKNQPPSIAATIFTAFFALW
                     GMPLMFTVAGFAIWHSLSKRTGQAFVVDRIHRLVVPFVFGVLTLVPLQVYFHLKQAEP
                     ASRITYWQFLQLFFDVRLCPDLVNAFICPDPATGLFTTAHLWFLKDLFIFSMLLLPLF
                     LYLGGQRGGNVIGKLGDFLARPGMIWLVALPVAGFEAGFVTSTMGGSWNEYTYAVLLM
                     AGFLIAAEPRLAQVMGRVWRSGLVIGLVVEAVYMVGLYLLMDVYDLDPLHGYDWGSLL
                     WRGIKSVGAWAWIVAIIGYGSRSRSKPIPEGVRKSTGTGNLVARPIAYAGEAFLAFYI
                     LHQTVIFVIGYYVVQWAIGALLKFIVISLSSLAVTLLLYELGVRRTKVTRWLFGMRIN
                     PPAPLADVPPGRSAGEVAV"
BASE COUNT          647 a          715 c          664 g          568 t
ORIGIN      
        1 aaacccggtt acttgactac cgtctatcga ccgcagagga acggttggtg cacgactttg
       61 cagtggactt ccccgggcag gacctggcgg cggtttggac acgctatgag ggcagcccct
      121 cgtttgatgg ccgctactgg ggattcatgg ctgaggacca ggaatggctg gctactgcct
      181 tgctggtcta cgatctgaaa attgatcgcg tggtcgccag gcgcgatctg ccctcccggc
      241 cggaaatcga ctctgtcagc atcagcccgt tgggaggttt cttcctcgcc tattacgacg
      301 gctactgcga acccgggcaa ttggggagcg acgcccaccc atgcggatta atggtctacg
      361 atcgtgacct tcaaaacggg cggggtttac tgcgcattgt tggtcactca gacctagcac
      421 tcgatgccaa tcaaagagag gtgctcatct tccaggatat cgacaccgac aatatctccc
      481 tcctgggtct ggaatccgga gaagttaccc ccttgtggcc gatcgatttc agccactcgg
      541 cacttggttt acatttctca gggcgggcca gccgcatgcc tggctgggcg ctggtttcga
      601 catacaacgg cgcacaacca gccgcgactt ggatggatga ccagatattc gtggtagaac
      661 tcaagccagg cgggcgtgtg gtgagactgg tgcataccta ctcaatcgtg gacgagaacc
      721 aggagcacga ttattgggcc gagccgcacg ccagtgtcaa tcacgacttc acacgaattg
      781 tcttcactag caattggggg cgatccggca caggtgaggt tgagacctat ctgattgagc
      841 taccgcccga ttggttatcg caattgccct aaatttcggt gttgggtagt ctatttctcg
      901 gccacaaaca aaaatcgtga caatcacctt tcaccggtaa gttagtggac gatagctttc
      961 attctaccag ggtgtaaaac tctcgactcc cactttccac aaggggcatc atgcgttcaa
     1021 acaagccagc gaagcggtca tcaccaaagg ttttcttggc atcagcttcc caggcgctaa
     1081 agctttcata gtcggcctcg gtgactaccg tgaagaacgg cccgcttaga tcggtcatga
     1141 tgcggacttt acgctcgctt ggccaaatgg cttggctatc cttcataagc tgtaccaatt
     1201 catcaccctt gccgtacttg gcttgaaaaa cattgcgtac gataatcact ttggacctcc
     1261 ttggttattc ggttgaatag caattgggga tatccgatag attggatttg tattatcaat
     1321 tatatcattt ctatctccgg ttaaacaggt caactccgct acactgctac ctcaccggca
     1381 cttcttcccg gcggtacatc agccaatggg gcaggcgggt ttattcgcat gccgaagagc
     1441 cagcgcgtca ctttggtccg cctgacaccg agctcgtata agagcagggt aaccgccagc
     1501 gatgataagc tgattacaat gaatttaaga agagccccaa tcgcccactg caccacgtaa
     1561 taaccgatga caaagatgac ggtctggtgc aggatataga aggcaagaaa ggcttctcca
     1621 gcataagcaa tagggcgggc aaccagatta cccgttcctg tggactttcg cacgccttca
     1681 gggattggtt ttgatcttga tcgcgaaccg taaccgataa tggcgacgat ccatgcccat
     1741 gcaccaacgc tttttatccc ccgccagagc aggctgcccc aatcgtatcc gtgaagcgga
     1801 tctagatcat aaacatccat cagcaaataa agtccaacca tgtagactgc ctcgaccacc
     1861 agaccaatca ccagcccgct ccgccacacg cgtcccatta cctgcgcgag gcgcggttcg
     1921 gctgcgatta agaatcccgc catgagcaaa accgcgtagg tgtactcgtt ccagcttcct
     1981 cccatggtgg aggtgacaaa cccggcttca aatccggcga ctggcaaagc tacaagccag
     2041 atcatcccag gtcgagccag aaagtccccc agtttcccga ttacattacc gcccctctgc
     2101 ccccctaggt aaagaaacaa aggcagcaat agcattgaaa agataaataa atccttcagg
     2161 aaccataaat gagccgtagt gaacaaccct gttgccggat cggggcagat gaaagcattc
     2221 accagatcag ggcatagtcg cacatcgaag aataattgga ggaattgcca gtaggttatc
     2281 ctggacgccg gctcggcttg cttgaggtgg aaatacacct gcaaaggaac caaagtcagc
     2341 actccaaaca caaatggaac gaccagcctg tggatccgat cgaccacgaa ggcctggcct
     2401 gtgcgcttgc tcagcgagtg ccatatggcg aagccagcaa cggtgaacat taacggcata
     2461 ccccacaagg caaagaaagc tgtgaaaatc gtcgccgcga tgctgggggg ctggttcttg
     2521 accccctctg gtaatgatat ggtgtcgaag atgcgggcgg tatgaaaaaa taccagtcca
     2581 aatacgagca ggac
//
LOCUS       DBNHOH010000600         3371 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1317878, whole genome shotgun sequence.
ACCESSION   DBNHOH010000600 DBNHOH010000000
VERSION     DBNHOH010000600.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1317878"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="AC16V7_10500"
     CDS             complement(<1..301)
                     /locus_tag="AC16V7_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HIR6257591.1"
                     /translation="MTKLTRREFLKLSGSAALSLAFTGLDLQLLEPIVVDNPLASYPD
                     RNWESIYRDQYRYDSSFTYVCSPNDTHACRLRAFVRNGIILRSETNYDVERYSDLY"
     gene            complement(316..660)
                     /locus_tag="AC16V7_10505"
     CDS             complement(316..660)
                     /locus_tag="AC16V7_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR6257592.1"
                     /translation="MDAHQVFIIGDSLLAETLAKLLVNDVTIQVEEIASSLEEALPRL
                     KANRPDVVIVAGVNRLSEAAFAHILSEIPDISILCIDLGTNDVQVVTNQRVSVHTTSD
                     LLAVIGALPKQV"
     gene            complement(824..1486)
                     /locus_tag="AC16V7_10510"
     CDS             complement(824..1486)
                     /locus_tag="AC16V7_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6257593.1"
                     /translation="MSPFRILLADDHDLFREGLASLINAQPDLEVVGQATDGLEALAL
                     AHEHHPDLIIMDIRMPVSDGLEATRLIRADWPEARIVILTVHDEDEKLFEAIKSGASG
                     YMLKNTNSAGFLEGVRSALAGEAPLPPKLGASLLEEFARLANKPSPTTPPPELTPRER
                     EILEHLAAGSSDKEIASQLALSLHTVKTHVRNILSKLHAVNRRQAADLATRAGWVKRK
                     NG"
     gene            complement(1491..>3371)
                     /locus_tag="AC16V7_10515"
     CDS             complement(1491..>3371)
                     /locus_tag="AC16V7_10515"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6257594.1"
                     /translation="FDSVQGRINLIFLAFSLLVVLSVGATIWEIETQRTDALIINLAG
                     RQRMLTQKITWLALAQPGSPELSASVQLFEQTLHALRHGGPAAYGAAAGLTTPGEAST
                     VVLPNAPDAALAAVLDEAARMWDDFRHHLEPLDVQELQAASPSLLAHLDRIVSQYEER
                     AEAKLGRVKLIQGISLLAGLALLMWGYLLTRKRIFAPLVQLGAAARLMEAGQLAEPVP
                     PIGDDELGKLVHAFESMRAEVASVHEHLESRVAQRTRELAAAFELSQEIVAQLELDRL
                     LRSVTERARTLTHARAAALCLLNQDGSMLVLEASSPEDEAHINLQQSLQREPARCVVC
                     TGEPVVAQAECLPCAFLHSQAPGQSAVAPLRAGETTIGALCVVRDSGQAFDRDETRAL
                     TLLSNSAAIAITNARLVETGRSQAEQAAKLSERERLAEELHDNLAQTLSFLNVQTERV
                     VGMVKTGDVEEGLSQLERMKEAIGGAYLQVRAALVGLSEPLPNADDFAHKLSENLDEI
                     RHATGLHVALEVADASALELPRMAQAQAIRIMREALANASKHSQAQNVRVCIERMNGQ
                     SRFIIEDDGRGFDPQDVQGNSHLGLRLMRARAERSGGTLEVESAPGKGTRVVVCFPIK
                     DA"
BASE COUNT          744 a          979 c          930 g          718 t
ORIGIN      
        1 cgtacaggtc gctgtaacgc tctacatcgt agttcgtctc agatcgcaag ataattccat
       61 tgcggacaaa agcccgcagg cggcaggcat gtgtatcatt gggagaacag acgtaggtaa
      121 aggaagagtc gtaacgatac tggtcccggt aaatgctttc ccaattgcga tccggataag
      181 acgctagtgg gttatccaca acaatgggtt cgagtaattg caggtccagt ccggtgaatg
      241 ccaggctgag ggctgcagac ccagacaatt tgagaaactc gcgacgggta agttttgtca
      301 tcaggccatc tccatttata cttgcttggg tagcgcaccg atcaccgcca gcaaatcact
      361 ggtggtgtgc acgctcacac gttgattagt gacaacctgc acatcgttgg tgcctagatc
      421 gatacacagg atcgatatat caggaatttc agagaggata tgagcaaagg ctgcctcgga
      481 tagccggttc acccccgcga cgatgaccac atcgggacga ttggctttta accgcggaag
      541 ggcttcttcg agcgatgagg caatttcttc aacctggatt gtgacgtcat tgaccagcaa
      601 tttcgccaac gtctctgcca acaggctatc accgataatg aaaacctggt gcgcatccat
      661 ataatcagaa tacagatatt ctcaggccaa gtctatccgt ctcattcggt acaaagaatg
      721 aaaaaagagc gatctctgga tcgctctgaa taaaaaagaa ttaggtcgaa attatcccga
      781 aggatcggag gcgtttttat gcttgggtct tcacccgaga ggcctagcca ttctttcttt
      841 taacccaacc ggcgcgagtt gccaaatcgg cggcttgacg gcggtttacc gcgtgtagct
      901 tgctcaagat gttgcgcacg tgggtcttca ccgtatgcag gctgagagcc agttgcgagg
      961 cgatttcttt gtcggaggaa cctgctgcca ggtgctccaa aatttctcgt tctcgaggcg
     1021 tcagctctgg tgggggtgtt gtgggcgagg gcttattcgc caggcgggca aactcctcca
     1081 acaggctcgc ccctaatttc ggtggcaggg gtgcttcgcc cgccagggcg ctcctcaccc
     1141 cttctagaaa tccagccgag ttggtatttt tcagcatata accgctggcc ccagatttga
     1201 tggcctcaaa cagcttctcg tcctcatcat gaacagtcag aatgacgata cgcgcttcgg
     1261 gccaatcggc gcggatcagg cgcgttgctt ccagcccatc ggagaccggc atcctgatat
     1321 ccatgataat caggtcaggg tgatgttcat gggccagggc caacgcttcc aacccatcgg
     1381 tagcctgacc aaccacttcc aagtcgggct gggcgttgat caggctggcc agtccctctc
     1441 ggaagaggtc gtggtcgtcg gcgagtagaa tgcgaaatgg tgacatcggt tcaagcatcc
     1501 ttgatgggaa aacaaaccac cacgcgcgtc ccctttcctg gagccgattc cacctccaac
     1561 gtgcctccgc tgcgctcggc gcgggcgcgc atcagcctga gacccaggtg gctgttgcct
     1621 tgaacatctt gggggtcgaa cccgcggcca tcatcttcta tgatgaaacg actctgtccg
     1681 ttcatgcgct caatgcagac tctcacgttc tgcgcctggg agtgcttgct ggcattcgcc
     1741 aatgcctcgc gcatgatgcg gatcgcctgt gcctgcgcca ttcggggcag ttctaatgca
     1801 ctagcatcgg caacctccaa agcaacatga agtccggtgg cgtggcgaat ttcgtccagg
     1861 ttctcagaca gcttgtgggc aaaatcgtcg gcgtttggca aaggctcact caagccgacc
     1921 agcgcggcgc gcacttgcag gtacgcccca ccgatcgcct ccttcatacg ctccagttga
     1981 gacaggcctt cttcaacatc gcccgtcttg accataccca ccacgcgctc agtttgcacg
     2041 tttaagaagc tcaatgtctg ggccaggtta tcgtgcaatt cttccgccag gcgctcgcgc
     2101 tcggagagct tggcggcttg ttcagcctgg ctgcgaccgg tttcaaccag acgggcgttg
     2161 gtgatcgcaa tcgcagcgga gttggacaac agcgtcaggg cgcgggtctc gtcgcgatca
     2221 aacgcctgcc ctgagtctcg caccacacat aacgccccta tggtagtttc tccggcgcgc
     2281 aatggagcca ctgcagactg cccaggcgct tgcgagtgca aaaaagcaca cggcagacac
     2341 tctgcttgtg ctaccactgg ctcaccagta cacaccacgc aacgggcagg ttcgcgctgg
     2401 agcgactgct gaagattgat atgcgcctca tcttccgggc tgctggcttc caaaaccagc
     2461 atagacccgt cttggttgag cagacacagc gcagccgctc tggcatgcgt caacgtacgg
     2521 gcacgctccg tgaccgagcg cagcaagcgg tctagctcca attgagcaac gatctcctgg
     2581 cttagctcaa aggcagcagc cagctcgcgg gtgcgctgag cgacgcgcga ttcgagatgc
     2641 tcgtgcacgc tggctacctc ggcgcgcata ctttcgaatg catgaactaa cttgcccagc
     2701 tcatcgtcac cgattggcgg gactggctca gcaagctgcc ccgcctccat gagtcgcgct
     2761 gcagcaccca gttgcaccag cggtgcaaag atgcgcttgc gggtaagcag gtaaccccac
     2821 atcaacaaag ccaagcctgc cagcagggag atcccctgaa tgagcttgac gcggcccagc
     2881 ttggcttcgg cacgctcttc atactggctg acaatacgat caagatgggc gagcaggctg
     2941 ggggaagcgg cttgcaactc ctggacatcc aatggttcca gatgatggcg gaagtcgtcc
     3001 cacatccggg cagcctcgtc caatacggca gccaatgccg catccggcgc gtttggtaaa
     3061 acgacggtac tggcttcacc aggggtcgtt aacccagccg ctgcaccata agccgctgga
     3121 ccgccgtgac gcaaggcatg caatgtctgc tcgaataatt ggacggaagc ggataactca
     3181 gggcttcccg gctgggccag ggccagccag gtgatcttct gggtgagcat gcgctggcgt
     3241 ccagccaggt tgatgatcaa cgcatccgtg cgctgagtct caatctccca aattgttgcc
     3301 cccacagaga gcaccaccag cagggaaaat gccagaaaga ttaggttgat cctaccttgt
     3361 acgctatcaa a
//
LOCUS       DBNHOH010000601         3099 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_677214, whole genome shotgun sequence.
ACCESSION   DBNHOH010000601 DBNHOH010000000
VERSION     DBNHOH010000601.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_677214"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..2013
                     /locus_tag="AC16V7_10520"
     CDS             100..2013
                     /locus_tag="AC16V7_10520"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR6257595.1"
                     /translation="MLLIGLLVVTTTAGAQTTASAILFPPQTQSFPRITTFMDVHDAQ
                     TSFVHGVQASEVQIIENGRSLPVVELRELRPGVQIVLALNPGPPFGIRDSQGISRYEF
                     IRDALLDWAKVRRGSSIDDLSLLISAGPEISHVSDTTELVSALGSYQPDSDNASPGLE
                     ILLRAIDIAADATPRAGMERAVLLVTPPLEGDISFGLQELISRAALLRVHVFVWLVAS
                     QQAFESQPAILLSRLSEQTGGQFFTFSGIEDIPNPEGYFEELRDIYQITYDSKITQGG
                     SQELAVEIRHGEQVITSPSLTFDFELSPPDPAFIFPSLEISRSIPAERRKNFWEQVNP
                     VDLKPDEQEIQVLIDFPDGRRRPLVRTTLYVDGVVVEENNEPPFEYFRWDLSAYTDSS
                     QHILQVEAVDSLGMNGTSIELPVQVNIDLPKPNPLSALFVNWPVLAGLAALLAGAIAL
                     LTLILTGRIRPHRLATFGLSGRRLIRKAKPPQPDATRAQRPRLETGGRYLPNWVNRLQ
                     WPQRRLNPNAYAYLTYLSDVVEKHGSTPISITADEITFGLDPSLATQVLEDPSVEGLH
                     ARLIRSEDGSILLVDQGSVAGTWVNYTEVPEGGRVLEHGDLIHFGRVGFRFSERNPRR
                     ARKLVITIEEPQS"
     gene            2010..>3099
                     /locus_tag="AC16V7_10525"
     CDS             2010..>3099
                     /locus_tag="AC16V7_10525"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF025053.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR6257596.1"
                     /translation="MIPAERAHLRVTAVSHPGMSGKNNEDRYGVSAYYTDAERETPAV
                     IAVVADGIGGHRAGEVAAEIAVETISAWVAESDASQPVDTLRHAIIHTGQVISEMAES
                     NSTLEGMGSTCACALIIGDQLYIATVGDSRIYLIRDNTIQLMTTDHTWVQEAIENGLL
                     GRDEARNHPNAHVIRRYLGSRQDVIPDTRLRLRSNESDTQAEENQGTRLQPGDILVLC
                     SDGLTDLVEDDEILEVLKTRNNQGALEDLVKMSNRRGGHDNVTIVTLAVPDVERPTTP
                     LIERQARKRLRSILIVAATLVLATITLLGGMLWYFARSDQEPTPMPSATQQVQATLFP
                     DQATQPGLFPTEAGIPGSASTALPGATVS"
BASE COUNT          678 a          919 c          859 g          643 t
ORIGIN      
        1 aaggtggaat atcagtactg cgggcttata gcgccgattt tataggctgc gatcttgtgc
       61 taaaataggc atatgccttt ccgttcgtgc tggagggcta tgctgctgat cgggttgctc
      121 gttgttacaa cgactgccgg agcgcagact accgccagcg ctattctatt ccctccacaa
      181 acccagtcct ttccgcgcat caccaccttc atggacgtgc atgatgctca gacgagcttt
      241 gtgcacggcg tccaggcctc tgaggtgcaa attatcgaga atgggcgctc gctacccgtg
      301 gtggaattac gtgagctgcg cccaggtgtg caaatcgttt tggctcttaa ccctggtcct
      361 ccctttggca tccgagatag ccaagggata tcaaggtatg agttcatccg agatgcgctg
      421 ttagattggg caaaggtgcg ccgcggctcc agtatcgacg acttaagcct gctgatctcc
      481 gctggacccg agatcagcca cgtctctgac acaacggaat tggtctctgc gcttggaagc
      541 taccagccag attcagacaa cgcatcgccc gggctggaga tcctgctgcg cgctatcgat
      601 attgctgctg atgccacacc aagagcgggg atggaacgtg ctgtcctgct ggtaacgccg
      661 cccctggagg gcgatatctc gtttggcttg caggagctaa tttcccgggc tgccctcctg
      721 cgcgtgcatg tattcgtctg gctggtcgcc tctcagcagg cattcgagag ccagccggca
      781 atcctgcttt cccgcctgtc ggaacagaca ggggggcaat ttttcacttt ctcaggtatt
      841 gaagacattc ccaacccaga aggttatttc gaagaactga gggatatcta ccagataact
      901 tatgactcca agatcacaca aggcggtagc caggaactgg cggtggaaat tcgccatggc
      961 gaacaggtga tcacttctcc ctccttgacc ttcgatttcg aactgagccc tcccgatccc
     1021 gcctttattt tcccatctct ggaaatctcc cgcagcatac cagctgaacg acgaaagaac
     1081 ttctgggaac aggtcaaccc tgtcgattta aagccagatg aacaagaaat ccaggtactg
     1141 atcgacttcc ccgatggtcg caggcgtccg ttggtacgaa ccacattgta cgtggatggg
     1201 gtagtggtgg aggagaataa cgagcctccc ttcgagtatt ttcgctggga tttgagcgct
     1261 tatacagaca gcagtcaaca catcttgcag gttgaagcgg tggacagcct gggcatgaac
     1321 ggcaccagca tcgaattacc tgtacaagta aatattgacc tacccaagcc aaaccctttg
     1381 agcgccttat tcgtcaactg gccagttctg gcaggtctgg cagccctgct ggcaggtgcc
     1441 atcgccttgc taactttgat cctgaccggg cgcatccgcc ctcaccggct agcgactttc
     1501 gggctttccg ggaggcgtct catccgcaag gcaaaacccc ctcaacctga tgcaacccgc
     1561 gctcaacgcc cccggctcga aacgggtggg cgctacctgc ccaactgggt caaccgcctg
     1621 caatggccgc agcgcagatt gaacccaaac gcatacgcct atctcaccta tctctccgat
     1681 gtggtcgaaa aacacggctc aacccccatc tccattaccg ccgatgagat cacctttggt
     1741 ctggatccca gcctggcaac ccaggtgtta gaagatccat ctgtagaagg gttacatgcc
     1801 cgcctgatcc gtagtgagga tggctccatc ctgctggtcg accagggttc ggtggccggc
     1861 acatgggtca actataccga ggtgcccgag ggcggccgag tgctggagca tggcgacttg
     1921 atccatttcg ggagggtggg tttccgcttt tcggagcgga acccgagacg agcccgcaag
     1981 ctcgtaatta ccatcgagga gcctcaatcg tgatccccgc cgaaagagcc cacctgagag
     2041 tgacggctgt cagccacccg ggcatgagcg gtaaaaataa cgaagaccgc tacggggttt
     2101 cagcctacta taccgatgcg gagcgtgaaa cgcctgcggt gattgctgtg gttgccgatg
     2161 gcatcggcgg gcaccgcgcc ggtgaggtgg cggctgagat cgctgtggag accatcagcg
     2221 cttgggtagc tgagagcgat gcgagccagc ccgtggacac cctgcgccat gcaatcatcc
     2281 ataccggtca ggtgatcagc gaaatggcgg aaagcaattc gaccttggaa ggtatgggct
     2341 caacttgcgc ctgcgccttg atcatcggcg atcaactata tattgccaca gtgggagact
     2401 cgcgcattta tctcatccgt gacaacacca tccagctaat gacgacagac catacctggg
     2461 tgcaagaagc catcgagaat gggctgctag gacgcgacga ggcacgcaac catcccaacg
     2521 cgcacgtcat tcgccgttac ctgggatctc gccaggacgt gatcccagat acgcgcctgc
     2581 gcttgcgttc caatgagagc gacacccagg cggaggaaaa ccagggcacc cgcctgcagc
     2641 caggtgatat cctggtgctg tgcagcgatg ggctaaccga cctggtggaa gacgatgaaa
     2701 tcctggaggt acttaagacg cgcaacaacc agggagcgct ggaagatctg gtcaagatga
     2761 gcaaccgccg tggtggccat gacaacgtga ccatcgtcac attggcggtt ccggacgtcg
     2821 agcgcccaac caccccgctg attgagcgcc aggcacgcaa gcgtttgaga tcaatcttga
     2881 ttgtcgccgc cacgctggtc ctggcaacca tcacactgct gggtggcatg ctttggtatt
     2941 tcgcgcgtag cgaccaggag cccaccccca tgccatcggc aacccagcag gtccaggcga
     3001 ccctcttccc ggatcaagcc acgcagccgg gtctcttccc aaccgaggca ggcattcctg
     3061 gcagcgcatc gacagccctc ccaggcgcta ctgtctcca
//
LOCUS       DBNHOH010000602         1611 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1253993, whole genome shotgun sequence.
ACCESSION   DBNHOH010000602 DBNHOH010000000
VERSION     DBNHOH010000602.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1253993"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1367
                     /locus_tag="AC16V7_10530"
     CDS             165..1367
                     /locus_tag="AC16V7_10530"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008235 - metalloexopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HIR6257597.1"
                     /translation="MRNPKTSAMSHLNKLSDEIGSRAIGTPGNQAAASYIHDVFAACD
                     LDVEVQDYACPAWEDLGASLTLNGEPLDVAANAFSPDCDVTAPLVPACTIAELEAADL
                     NGRIGLLYGDLTTAPLACKSWFLKTERDDQIIRLLEEKEPAALITVQTKAGLLERLIE
                     DWEFLIPSATVPAMIGLALLRQSGSRVHLRIDSRQAPGSTANLVGRKPGNNPEKIVLC
                     AHYDTKFDTPGASDNGGGAAVLLALAERFRHEKMNYGLEFVAFTGEEYLPIGDDEYLR
                     RSGDGLGQIVAAVNFDGVGSLLGPNTITLIAASQPFQEHLNRLTAPHPGVAWVPPWPE
                     SNHSTFSMRGIPSIAFSSIGAFRLAHMRDDTVEWISEARLDEAVALSSEIIDSLQDKP
                     LAWGRQPE"
BASE COUNT          365 a          493 c          434 g          319 t
ORIGIN      
        1 agaacattac acctgccagc taaataaatt tcgtctgcct gcgggcgcaa aggatgtaca
       61 atagaaaacg tcccgcaggt ttccctaaaa caaacctgtg tgagcatgaa cctgcgggac
      121 gttcataacc aagctatctg aaggtcaatt aataggagac cgctatgcgc aaccctaaaa
      181 ccagcgccat gtcccacctc aacaagctgt cagacgagat cggctcccgc gccatcggca
      241 cgccaggcaa ccaggctgcc gccagctata tccatgacgt gttcgccgcc tgcgacctgg
      301 atgtggaggt gcaggactat gcctgccccg cctgggaaga cctgggtgcc agcctgaccc
      361 tgaatgggga gccgctggat gttgccgcca acgccttctc gcctgactgc gatgttaccg
      421 ccccgttagt acctgcctgc actatagctg agctggaagc cgctgacctg aatggtcgca
      481 tcggcttgct gtacggtgac ctgaccaccg ctcccctggc atgcaaatcc tggtttctaa
      541 aaactgaacg tgatgaccag atcatccgct tgctggaaga aaaggaaccg gcagccttga
      601 ttaccgtcca gaccaaagcc ggcttgttgg agcgcttgat tgaagactgg gagttcctta
      661 tcccttctgc gacagtgccc gctatgatcg gcctggcatt gctgcgccaa tcaggctccc
      721 gtgtgcatct gcgcattgac agccggcagg cgcccggctc caccgccaac ctggtgggtc
      781 gcaaaccagg taataaccca gaaaagatcg tgctgtgcgc ccattacgac accaagttcg
      841 atacaccagg agccagcgat aacggtggtg gggcagcggt tttgctggcg ctggcggagc
      901 gtttccgcca cgaaaagatg aattacgggc tggagttcgt cgccttcacc ggcgaggaat
      961 acctgccaat tggcgacgac gaatacctgc gccgtagcgg cgacgggttg ggccagatcg
     1021 tggcagcggt caacttcgat ggggtggggt cgctgctggg gcccaatacc atcaccttga
     1081 tcgccgcctc ccagccattt caggaacatc tcaaccgcct caccgcgccc catcccgggg
     1141 tggcttgggt gcctccctgg cccgagagca accactccac attttccatg cggggcatcc
     1201 ccagcatcgc cttcagctca ataggggcct tccgtctggc ccacatgcgc gacgatacag
     1261 tcgagtggat cagcgaggcc cgcctggatg aggcggtcgc cttgagcagc gagatcattg
     1321 acagcctgca agataagccg ctggcctggg gtcgccagcc ggagtaatat agatcacgcc
     1381 aaacaggcgc ctgttattta ggataaaatc tcaacacaac ccgttaattc acaaccaaaa
     1441 ctatcttcta ctggtaaact ctatttatga aaatcttaac ggtagacgtt ggcactggaa
     1501 cccaagacat ctttctctac gattcgcgca tcgacatcga aaacggcttc aagctggtcg
     1561 tcccgtcacc gaccatgatc gtgaggcgcc gcctgcaaga agccacgcgg c
//
LOCUS       DBNHOH010000603         2212 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1638372, whole genome shotgun sequence.
ACCESSION   DBNHOH010000603 DBNHOH010000000
VERSION     DBNHOH010000603.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1638372"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="AC16V7_10535"
     CDS             <1..255
                     /locus_tag="AC16V7_10535"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR6257598.1"
                     /translation="ETVAAMQAMQMSTDGSPLGFGLSWWTGKDDFGDFYYHVGDGAGS
                     EGTMRIYPDLDLGVVVMSNARGYQRDRIVAGLVSAWMHEK"
     gene            305..583
                     /locus_tag="AC16V7_10540"
     CDS             305..583
                     /locus_tag="AC16V7_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257599.1"
                     /translation="MKNAQPLFDTSTNYRIEVRGRVDVDWLQNFNSSVEIISDETRQL
                     ENITVLNVQTDQSGIVGLVRRLHALGITILQLQIVPDGDKVAKVEEQS"
     gene            769..1647
                     /locus_tag="AC16V7_10545"
     CDS             769..1647
                     /locus_tag="AC16V7_10545"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_function: GO:0140657 - ATP-dependent activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6257600.1"
                     /translation="MKLTIKHLSKQYRRDFWGLREFDLEVGPGVIGLLGPNGAGKSTL
                     MRMLATITQPSEGTIKWNDVDIVRSPDTLRAALGYLPQDFGIYPNLSGREFLEYIAAI
                     KGLDGNAARRRIDELLVVVNLVDAAKRPLGSYSGGMKQRVGIAQALLNDPQLLIVDEP
                     TAGLDPEERVRFRNLLSDLSGERIIFLSTHIVSDVEATATEIVIIHKGRKLLHAAPEK
                     ILQSLDGQVWQWVVPSEALVALKQKHIISGTVRRQDGIQVRVVSETAPSADAQPIAPS
                     LEDVYLQLVSGNGVMR"
     gene            1644..>2212
                     /locus_tag="AC16V7_10550"
     CDS             1644..>2212
                     /locus_tag="AC16V7_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257601.1"
                     /translation="MNSLRALYHMARADFLERVRRYSFLIMLGMIVWLGYVVNTGVLV
                     MSVPPNYVGEINSPWVGALMTITVTFFLGWFGFYMVKGSVGRDYETGVGQIMATTPLT
                     RPLYALGKWLSNFASLSIMSLILIIAGILMNLLLGSTAIDLWALVAPLLIISMPFMAI
                     VAAVAVLFETIGWLRGGLGNIVYFFMFILL"
BASE COUNT          502 a          576 c          621 g          513 t
ORIGIN      
        1 gaaaccgtcg ccgccatgca agcaatgcaa atgtctacag acggttcccc ccttggcttc
       61 ggcttgagct ggtggaccgg gaaagatgac ttcggcgatt tctactatca cgttggcgac
      121 ggggccgggt ctgaaggcac gatgcgcatc tatcccgacc tggacctggg tgtggtggta
      181 atgagtaatg ccagaggcta ccaacgagac aggattgtcg caggtctggt aagcgcctgg
      241 atgcacgaaa aatgaccttt cgtcacgcat ttcaagagaa caaggaacaa aaccgaggca
      301 aactatgaaa aatgctcaac ccttattcga cacgtcgaca aactaccgga ttgaggtgcg
      361 gggtcgagtg gatgtggact ggctgcaaaa cttcaacagt tcggtggaga taatctctga
      421 tgagaccagg cagttggaaa atatcaccgt gctcaatgtg cagaccgatc aatcggggat
      481 tgtcgggctg gtgcgcaggc tgcacgcact gggaataacg atcctgcaat tgcagatcgt
      541 tccagatgga gacaaagttg ctaaagtcga agaacaaagc taagtaccga cggctccagg
      601 aagcgtacat ttagcagctt aaaatcgcat acctgaaata tgtgggcgcg cacgcggaac
      661 aaaatcggcc caccagtata tatctgagcc ctgaaagcgg gcaatataga gaatttgtct
      721 tgggtaataa ttctacccgc tatacagatt gacgaaagaa ggttttaaat gaaactaacc
      781 atcaagcatc tttccaagca atatcgccgt gacttttggg gactgcgcga gttcgacctt
      841 gaagtaggac caggcgtgat cggcttgctc ggaccgaacg gcgcaggcaa gtctacactg
      901 atgcgcatgc tggcgacgat cacccagcca agcgaaggta cgatcaaatg gaatgacgtg
      961 gatattgttc ggtcgcccga tacactgcgg gctgcgctcg ggtacctgcc gcaggatttt
     1021 ggtatttatc caaatttgag cggccgggag ttcttagaat acatcgctgc catcaagggc
     1081 ttggatggca atgccgcccg ccgccgcatt gacgaattgc tggtcgtagt caaccttgtg
     1141 gatgccgcca agcgcccgct tgggagctac tcgggcggga tgaaacagcg tgttgggatt
     1201 gctcaggcgc tgctcaacga tccacagctg ctcatcgtag acgagcccac cgctgggctc
     1261 gatcccgaag aacgcgtgcg cttccgcaac ctgctctccg acctgtcggg cgagcgcatc
     1321 attttcctgt ctacacacat tgtttcggat gtggaagcga ccgcaaccga aatcgttatc
     1381 atccacaaag ggcgtaaact tctacatgcc gcgcccgaaa aaattttgca atcgctggat
     1441 ggccaggtct ggcaatgggt cgtcccttct gaagcgttgg ttgctcttaa acagaaacac
     1501 attatcagcg ggacagtccg ccgccaggac ggcatccagg tgcgggtggt gagcgagacc
     1561 gctcccagtg ccgacgccca gcccatcgcg cccagcctcg aggatgttta tctccaactg
     1621 gtttccggga acggagtgat gcgatgaact ccctgcgcgc actctaccac atggcgcgcg
     1681 ccgattttct ggagcgcgtg cgccgctaca gctttctaat catgcttggc atgatcgtct
     1741 ggctgggcta cgttgtgaac acgggggtct tggtgatgag cgtgccgccc aactacgtgg
     1801 gcgagatcaa ctcgccctgg gtgggcgcgc tgatgacgat cacggtcacg ttttttttgg
     1861 gctggtttgg cttttatatg gtcaaaggtt cggtcggccg tgattatgag actggcgtcg
     1921 gacagatcat ggcgaccact ccgctcaccc gtccgcttta cgccctaggc aaatggttga
     1981 gcaactttgc tagtctcagc atcatgagtc ttatcctgat aatcgctggc atcttgatga
     2041 atttgctgct cggctccact gccatcgatc tatgggcttt ggttgcccca ttgctgatta
     2101 tctcgatgcc atttatggcg attgtcgccg ctgtggctgt cctgtttgaa accattggct
     2161 ggctacgtgg cggtctgggc aacatcgtat actttttcat gtttatcctg ct
//
LOCUS       DBNHOH010000604         1772 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1638383, whole genome shotgun sequence.
ACCESSION   DBNHOH010000604 DBNHOH010000000
VERSION     DBNHOH010000604.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1638383"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1446)
                     /locus_tag="AC16V7_10555"
     CDS             complement(<1..1446)
                     /locus_tag="AC16V7_10555"
                     /inference="COORDINATES: protein motif:HMM:NF025700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HIR6257602.1"
                     /translation="MKKQLVHLTIVIITCLLLVGLSGCAETSSQSPDSTSSQMVNSGS
                     WYTRKQWPHDGHTYTTTNFIIFSDAASQEARETLASVAEKVLVEVITEFEIIPEEMLR
                     FPPGQDKIHIYAYKNYHPKEWGMRAYYAGLIAWSLDHEERSSDMEVYQSVLKHELVHV
                     IQNLLVGSDPNLAPLRLRVDAWFSEGLAESMTGGTSGGAITSHAQLNALAAEYGEFNP
                     MSYTSDLVLNAAMVNHPTIGFDYYYPISQLAVEYLFSAEGLGKSPADARDILIDVAED
                     INFATAFETHMGISIKKYKAEFFDRIDGYLEEGFSFPGPSLMLAWVILSALSIILVGI
                     SLTRSKSSVEGIQWVWLLITALYGPLGGLSYFISIRKQRQESSFWWRALVTSMVNVTG
                     KAIGILIVILALFFFAPDKDSGPLILLIPLLISWLIFQAPLLTAQVGVSYWAALRRSF
                     VAEFVSLVLVSAGMLPVLIWLPDRVWYFSNDP"
BASE COUNT          457 a          425 c          466 g          424 t
ORIGIN      
        1 gggatcattt gagaagtacc agacccggtc gggcaaccaa atgagcactg gaagcatgcc
       61 tgcagaaacg agcaccaatg agacgaactc agccacaaat gagcggcgga gcgcggccca
      121 atagcttaca ccaacttgtg ctgttaatag cggtgcctgg aagatcagcc aactgattag
      181 cagcggtatg aggaggatga gcgggccact gtctttatca ggcgcaaaaa agaataaggc
      241 gagaatgacg atcagtatcc cgatggcctt tccggtcacg ttcaccatgg aggttacaag
      301 ggcgcgccac cagaatgagc tctcctgccg ttgctttcgg atggaaatga agtagctaag
      361 cccaccgagc ggcccataca gcgctgtaat cagcagccat acccactgga ttccctcgac
      421 gctgctcttg ctgcgggtaa ggcttatgcc caccaggatg atggatagag cgctcaagat
      481 cacccaggca agcatcagac tggggcccgg gaaagaaaat ccctcctcca ggtagccatc
      541 aatgcgatcg aagaactctg ctttatattt cttgatagag atgcccatat gggtttcgaa
      601 agcagtcgcg aaatttatgt cttcagccac atcaatcaag atatccctgg catccgctgg
      661 ggattttcca agaccttccg cagagaacag gtactccaca gccagctgtg atatcgggta
      721 gtagtagtca aacccaattg tgggatgatt aaccatggca gcgtttagta ctaaatcgct
      781 tgtgtagctc atcgggttga attcaccata ctcggcagcc agggcgttaa gctgggcatg
      841 gcttgtgatt gcaccgccag aagtcccgcc cgtcatcgac tcggcaagtc cttcgctaaa
      901 ccaggcatca actcggagcc gcaacggtgc cagatttgga tcgctgccca ccagcaggtt
      961 ttggatcaca tggaccagtt cgtgttttag gacagattga tacacttcca tgtcagaact
     1021 gcgctcttca tggtccagtg accaggcgat caggcctgca tagtaggctc tcatgcccca
     1081 ttctttcggg tggtaatttt tgtaggcata gatgtgaatt ttgtcctggc cgggagggaa
     1141 acggagcatt tcctcgggga tgatctcaaa ttcggtaatc acttctacga gcaccttctc
     1201 cgcaacgctc gccagggttt cacgcgcctc ctggctggca gcgtcactga agatgatgaa
     1261 atttgtggtc gtgtaggtat gcccgtcgtg cggccattgt ttcctggtat accatgaacc
     1321 gctgttgacc atctgggatg aggttgaatc aggggattga gatgaagttt cggcacagcc
     1381 ggaaagcccc actagcagca gacaggttat gataacaata gtgaggtgca ctaactgttt
     1441 tttcataaat cctcctgaaa atgtacctgt cggtttatta atttgttttg gttgtgtaat
     1501 agaaatgtat gattatgttt ttattatata caagtagcaa aagcaatgtc aatttccagc
     1561 tgtgcggtgt ccaggagata ctatacactt tatgaggttc gaataccagg aatatgttga
     1621 ccgattggta gaattggcag ggatcaagcc acgaacagaa tggacaggct atgactcctg
     1681 gaatccgtat cgattacaag accctgagaa gcgtgaaccc tgaggccgca cgtcaagcgg
     1741 tgctggaata tctcaagacg aatggtggga at
//
LOCUS       DBNHOH010000605         2146 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_934217, whole genome shotgun sequence.
ACCESSION   DBNHOH010000605 DBNHOH010000000
VERSION     DBNHOH010000605.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_934217"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="AC16V7_10560"
     CDS             <1..351
                     /locus_tag="AC16V7_10560"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HIR6257603.1"
                     /translation="RQAIEGQIHGGVMMGLGYALSEQFVVEKGINLTDTLYKCRIPTA
                     DQTPEITCVIVEVPHPYGPEGVKGFAEAPSLATAPAIINAIYDALGVRITSLPADKKQ
                     VLRALQNQDKEKLP"
     gene            351..>2146
                     /locus_tag="AC16V7_10565"
     CDS             351..>2146
                     /locus_tag="AC16V7_10565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR6257604.1"
                     /translation="MEPSDLQVILHQVGKSQPRVDGYEKVIGQAKFIADLNLGRVLHA
                     RVVRASHAHAIIRSLDSRRALAVPGVKAVVTGETCAQRIGHAIADQYPIARGKVRYWG
                     EPVAVVVASTPEAAEKAAQLVDIDYERLPVSLHPRQAAQPGAPLIHEHLADYKVDSLI
                     HPIPGSNVCHHYRLSRGDVGQAFEQADVIVENEYWVPWIAHVQLEPHGAIAQWDGESF
                     NIWSSSQSPFFIRETISKLLDISPAKVRVIIPYVGGGFGGKSDVTIEPLLAVTAQAVP
                     GLPIQLVLTREEVFFGTVVGRGAWGRIKTGVSADGLLLAEQAELYFGSGGYADYSVWI
                     SQGGGHNATGPYAIPNLSLDSYSVYTNTPPTGAYRGYGHPEVHWMVERQMDQIARHLG
                     MDPFELRLKNLLLPGKVNALGQVMQDYNGRADLCLKAVQESLEGKSHSSTPGRARGRG
                     VACFMKSPVMRTNAQSGAIVRFNEDGSVTVYTGSVEIGQGVNTVLSQIAAETLSLPVE
                     MVHYSPRVDTDFSPHEWQTVASHTTWAVGNAVRMAAEDALEQIKAAAAQVLGVPLENL
                     ITRDAKVYPSGQEQLAIPFAKFATGYRLPNGAA"
BASE COUNT          458 a          575 c          664 g          449 t
ORIGIN      
        1 cgacaggcca ttgaaggcca gattcatggc ggggtgatga tgggcctggg ttacgcactc
       61 tccgaacaat ttgtggtcga gaagggcatt aatctcaccg atacgctgta taaatgccgc
      121 atccccacag cagatcagac acccgagatc acgtgtgtga ttgtggaagt gccgcatcca
      181 tatggacctg aaggggttaa aggatttgcc gaagcgccgt ctctagcgac ggcacccgcc
      241 attattaacg ccatctatga tgccctgggt gtgcgcatca catctctacc ggcggataaa
      301 aagcaggtgc tgcgagcgct tcagaatcag gacaaggaaa agctaccata atggagccga
      361 gcgatctgca agtcatcttg caccaggtgg gtaaatctca gccccgtgtg gatgggtatg
      421 aaaaagtaat cggccaggcc aagtttatcg ccgatctgaa cctgggccgg gtgctgcacg
      481 cgcgcgtggt gcgggcaagc catgcgcatg cgattatccg cagcctggat agtcggcgag
      541 cgttggcagt cccaggggtt aaagcggtcg tcactgggga gacgtgcgcg cagcggatcg
      601 gccatgcgat tgccgatcaa tatcccatcg cgcgaggcaa agtgcgttac tggggggagc
      661 cggtagcagt ggtagtggct tctacaccgg aggcggccga gaaagcagcc caactggtgg
      721 atattgacta tgaacggctg cccgtcagtt tgcacccgcg gcaggctgcc cagcctggtg
      781 cgccccttat tcacgagcat ctggcggatt acaaggttga ctcgctcata cacccgatcc
      841 ctgggagcaa tgtctgccat cactaccgct tgagccgcgg cgacgttgga caggcttttg
      901 aacaggcaga tgtgatcgta gaaaacgagt actgggtgcc gtggatcgct catgtccagc
      961 ttgagccgca cggtgcaatt gcccaatggg atggggagtc gttcaacatt tggtctagct
     1021 cgcaatcgcc ctttttcatc cgtgagacga tctcaaaatt attggatatc tcgccagcca
     1081 aggtgcgcgt gatcattccc tatgtgggag gtggttttgg cgggaaatct gacgtcacca
     1141 tcgagccgtt attggctgtc actgctcagg cggtgcccgg cttgcccatt caacttgtat
     1201 tgacccgtga ggaggtattt tttggcactg tggttggccg gggagcctgg ggtcgcatca
     1261 agactggtgt cagcgccgat gggctgctgc ttgccgagca ggctgagcta tatttcggca
     1321 gcggtggtta tgcggattat tcggtgtgga tcagccaggg tggggggcac aatgccactg
     1381 gcccgtacgc cattcctaat ttaagcctgg actcttacag cgtgtatacc aacacgcccc
     1441 caactggcgc ttaccggggc tatggacacc ctgaagtaca ctggatggtc gagaggcaga
     1501 tggaccagat tgctcgccat ctcggcatgg acccgtttga actgcgcctg aaaaacctgc
     1561 tactaccggg caaggtgaac gccctggggc aagtgatgca ggattacaat ggccgcgccg
     1621 atctatgcct gaaagcggtg caagagtctc tcgaagggaa atcacattcc tccacaccag
     1681 ggcgcgcccg cgggcgaggc gtggcgtgct ttatgaaatc accggtcatg cgcacgaacg
     1741 cccagagtgg agcgatagtg cgcttcaacg aggatggttc cgtcacggtt tacacgggtt
     1801 ccgtagagat cgggcaaggg gtgaacaccg ttctcagcca gattgcagcg gagacgcttt
     1861 cgttgccggt ggagatggta cattattcgc cgcgggtcga tacggatttt tcgccgcacg
     1921 aatggcagac cgtcgccagt cacaccacct gggcagtggg caacgcggtc cgcatggctg
     1981 ctgaagatgc gctggagcag atcaaagcgg ctgctgcgca agtcttaggg gtgccgctgg
     2041 aaaacctgat caccagggat gctaaagtgt atcccagcgg tcaggagcag cttgctatcc
     2101 cattcgccaa atttgccacc ggctaccgcc tgcccaacgg cgcggc
//
LOCUS       DBNHOH010000606         4187 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_741803, whole genome shotgun sequence.
ACCESSION   DBNHOH010000606 DBNHOH010000000
VERSION     DBNHOH010000606.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_741803"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="AC16V7_10570"
     CDS             <1..283
                     /locus_tag="AC16V7_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257605.1"
                     /translation="RAISHIAGRIILVRWWLLGTLTLVLVGCVTTLTNPKVEIMDTFT
                     SPGGPFRADLDDLGMAPELDNEVWLNTESPLRLADLRGKVVLLDMWTFG"
     gene            299..613
                     /locus_tag="AC16V7_10575"
     CDS             299..613
                     /locus_tag="AC16V7_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIR6257606.1"
                     /translation="MIPSLREWHESYGPEGLVVIGNHYPEFDYEADLENLKEAVKALE
                     VPYAVAQDNDGETWRAYHNRYWPTLYLIDKWGHIRYRHIGEGRYDETEAAIQALLKET
                     FP"
     gene            741..3794
                     /gene="glyS"
                     /locus_tag="AC16V7_10580"
     CDS             741..3794
                     /gene="glyS"
                     /locus_tag="AC16V7_10580"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HIR6257607.1"
                     /translation="MTKTLDFQSIIMALQDFWAQQGCLIWQPYYTQVGAGTMNPATAL
                     RVLGPEPWNVAYVEPSIRPDDGRYGENPNRMQMHYQFQVILKPDPGNPQEIYLKSLQA
                     IGIDPRQHDIRFVEDNWESPALGAWGLGWEVWLDGQEITQFTYFQQAGGQILDPVSVE
                     LTYGLERIAIALQRVGSFREIQWSPTLTDGDVNLQAEQEHSQYYFEVADVNRLRQMYD
                     VFEAEAEACLAQNLVLPAHDYVLKCSHTFNVLDTRGAIGVTERAAFFGRMRDLSRRVA
                     EAYIEQRQRLEFPWLNHLETGETTVPAPHSSSPITYSLSPATFLLEIGTEELPSRDLD
                     AAIEQLRSRMAAMLDTLRLEHGELLVQGTPRRLVVYVEQLAPRQSDLEQVVKGPPAER
                     AFNPDGTPTQAAEGFARSKGIAVSDLQVREMDGGRYVVAVVRQEGLPAHSVLAEALPD
                     LIASLRFDKSMRWNSTNVAFSRPIRWLLALLGDQVVPFSYAGLGAGRSTRGLRFHAPA
                     EISLKTTQDYFNALKAQDIILDVEERRTYIQNQIESLAAEVGGVIAPDSDLLAEVANL
                     VEAPIALRGSFDPSHLELPREVLISVMKKHQRYFPVFEASESTDETAPDIQKPVQPLL
                     PYFVAIRNGDDLGLNLVVEGNEHVIRARFADAAFFVRDDSKRHLQEYLPQLKTLSFQK
                     KLGSMLDKTQRISEMLTDLAPLVGLSRDEMAIAGRAAELCKADLATKMVVEMTSLQGL
                     MGRHYALISGEPSEVANAIFEHYLPRFSGDEIPGERPGLVVGLADRLDTLAGLFAAGL
                     APTGNKDPFAQRRAALGLVQNLIAWELPFDLRQALELAAKRLPIPASHESQKACLEFI
                     IERLRNLLLEQGWRYDVVDAVLVEQGHDPARAAQAVKSLAAWVSRPDWSTILPAYARC
                     VRITRDQAEHFPVDRSKFVERAEGELFSALLTAESASAENRQHGSPDIFLNALLPMIP
                     AIDQFFDDVLVMTEDIAIQHNRLGILQRISALADGVADMSRLEGF"
     gene            complement(3802..>4187)
                     /locus_tag="AC16V7_10585"
     CDS             complement(3802..>4187)
                     /locus_tag="AC16V7_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257608.1"
                     /translation="GRYEEAAFLRELFDEPTTLLYQIWSLIRTIDDCFKPNSSIGIED
                     LLTQTDQLILAIIKTLEGDQEAEIVRLLLRLRDSLSRQDDTQILNAQSEAARAEVINV
                     VNNFFHERLMAVPTIKSYIESMSTK"
BASE COUNT          913 a         1139 c         1165 g          970 t
ORIGIN      
        1 tagagcaatt tcccacattg cagggagaat tatacttgtg agatggtggt tattaggaac
       61 gcttacactt gtgttggttg gatgtgttac tacactgacc aacccgaagg ttgagatcat
      121 ggatacattt acctcgcctg ggggaccatt ccgcgccgac ctggatgatc tcgggatggc
      181 gccagagctg gataatgagg tctggctcaa caccgaatcc ccattgcgct tagctgatct
      241 gcgcggcaag gtggtgctgc tcgatatgtg gacctttggc tgaatcaact gccgtcgcgt
      301 gattccctcg ttgagggaat ggcatgaaag ttatggccca gaggggctgg tggtgatcgg
      361 caaccactac ccagaattcg attacgaagc cgatcttgaa aatctaaaag aggcagtcaa
      421 ggcattggaa gtgccctacg cagtcgccca agacaacgat ggcgaaacct ggcgggcgta
      481 tcataaccgc tactggccta cgctgtattt gattgacaaa tggggtcata tccgctatcg
      541 ccatatcggc gaaggaaggt acgacgagac cgaggcggct attcaggcgc tcctaaagga
      601 aacattcccc tagaacgcgt gacctcccca aggtgctaca agacccatca tcatttacgg
      661 gcagcattta gatgctgccc atttttacgt gtataattcg ggcgaatatt cacaccagta
      721 tctgcgattt actacactca atgacaaaaa ctcttgattt tcaatccatt atcatggcct
      781 tgcaagactt ttgggctcag cagggatgcc tgatctggca gccatattac acccaggtgg
      841 gcgctggtac gatgaatcca gcgactgcgc tgcgcgtgct tggaccagaa ccgtggaatg
      901 tcgcctatgt cgagccgtct atccgccccg acgatggtcg ttatggcgag aatcccaatc
      961 gcatgcagat gcattaccag ttccaggtga tcctcaagcc tgatccaggc aacccccaag
     1021 agatttatct gaaatcatta caggcgattg gcattgaccc gcgccagcac gacatccgct
     1081 ttgtagagga taattgggaa tcgcctgccc tgggtgcctg gggacttggc tgggaggtct
     1141 ggctggacgg acaggagatc acccagttca cctatttcca gcaggcaggt gggcaaattc
     1201 tggatccagt ctcggtggag cttacctacg ggctggagcg aattgcaatc gccctgcagc
     1261 gcgtgggcag cttccgcgag atacagtgga gcccaacgct caccgacggc gacgtgaacc
     1321 tgcaagctga gcaagaacac agccaatatt acttcgaagt tgctgatgtc aaccgactac
     1381 gccagatgta tgatgtgttc gaggctgagg cggaggcctg cctagcacag aatcttgtgc
     1441 tgcctgccca cgattatgtg ctgaagtgct ctcacacgtt caatgtactg gatacgcgtg
     1501 gcgccatcgg cgtaactgag cgtgcggctt tcttcggacg catgcgcgac ctgtcgcgca
     1561 gggtggcaga agcctatatt gagcaaagac agcgccttga gttcccctgg cttaatcatc
     1621 tagagacagg ggaaacaaca gtgcctgccc ctcattcatc gtcccctatc acttactctc
     1681 tctcccctgc cactttcttg ctcgagatcg gcacggaaga gcttccttcc cgagatctgg
     1741 atgcggcgat agagcaactg cgctctcgta tggctgccat gttggacacg ctgcgtcttg
     1801 aacatggcga gctgctcgtc cagggcacgc cgcgtcgcct ggttgtttat gtagaacaat
     1861 tagctccacg tcagagcgac ctagagcaag tagtgaaagg tccgcctgcc gagcgcgcct
     1921 tcaatcccga tggcaccccc acccaggctg ccgagggttt cgctcgcagt aaggggatag
     1981 cggtgagcga cctgcaggtc cgggagatgg acggcgggcg ctatgttgtt gcggtggtcc
     2041 ggcaggaagg cctcccagcc catagcgtgt tagcagaggc attgcccgat ctgatcgcct
     2101 cactgcgttt tgacaagtcg atgcgctgga acagcaccaa tgtggctttt tcccgcccaa
     2161 tccgctggct gcttgccttg ttgggcgatc aggtagtgcc cttctcgtat gcaggtttgg
     2221 gcgcaggccg ttccacgcga ggtctgcgtt ttcacgcgcc ggctgagatc agcttgaaga
     2281 ccacccagga ttactttaac gctttgaaag cacaagacat catcctggat gtggaagagc
     2341 gccgcacata cattcaaaat caaatcgaaa gtttagcggc agaggtcggc ggggtaattg
     2401 caccagatag cgacctgctt gccgaggttg ccaacctggt agaagccccc atcgcgctgc
     2461 ggggctcctt cgatccatct catctagagc tcccccgcga ggtgctgatt tccgttatga
     2521 aaaagcacca gcgatatttc cccgtttttg aggcttccga gtcgactgat gaaacggcgc
     2581 cagatataca aaaaccagtg caaccattat taccttattt cgtagccatt cgcaacggcg
     2641 atgaccttgg gttgaacctg gtggtggagg gcaacgagca tgttatccgc gcccgctttg
     2701 ccgacgcagc cttcttcgtg cgtgacgata gcaagcgcca cctgcaagag tatcttcccc
     2761 agttgaagac tctctccttc cagaagaagc tcggctcgat gctggataag acgcaacgca
     2821 tcagcgaaat gttgacggac ctggctcccc tggtggggct cagtcgtgac gagatggcca
     2881 tcgctggacg cgccgccgag ttatgcaagg ctgacctggc aaccaagatg gtcgttgaga
     2941 tgacttcatt gcaggggttg atgggccgtc actacgcttt gatctcaggt gagccgagtg
     3001 aagtggcaaa tgccattttt gagcattacc tgccgcgttt ctcgggcgac gagattcctg
     3061 gagaacgccc tggtttagtg gttgggttgg cagaccgact ggatacattg gcaggcctgt
     3121 ttgcggctgg gttagcgccc actggcaaca aagatccctt cgctcagcgc cgcgcagccc
     3181 tgggtttggt acagaacctg atagcttggg aactgccctt cgatttgcga caagctctgg
     3241 agttagctgc aaagcgcctg cccatccctg caagccatga gagtcaaaaa gcttgcctgg
     3301 agtttatcat tgagcgcttg cggaacctgt tgctggaaca gggctggcga tatgatgtag
     3361 tcgatgctgt gttggttgaa cagggtcacg acccagctcg agcagctcag gcggtcaaat
     3421 cactggctgc ctgggtgtca cgcccagatt ggagtaccat cttgcctgcc tacgcccgct
     3481 gcgtgcgcat cacccgcgac caagccgagc acttcccggt cgatcgaagc aagttcgtcg
     3541 agcgcgccga aggtgagctc ttctccgctt tgcttacagc cgaatccgca tcagcggaga
     3601 ataggcagca tggatcgccg gatattttcc tgaacgctct cctgcccatg atcccggcca
     3661 tcgaccagtt ctttgatgac gtccttgtga tgaccgaaga cattgccatt caacacaacc
     3721 gcctggggat cctgcagcgg atatctgcat tggcggatgg cgtagcggat atgagccgcc
     3781 tagaaggctt ttgatatgct cttatttagt tgacatactc tcaatatatg acttaatggt
     3841 cggcacagcc atcagtcgct catggaagaa gttattgacc acgttgatca cttctgccct
     3901 agccgcctct gactgtgcat taagtatctg cgtgtcatcc tggcgggata ggctatcgcg
     3961 caggcgcagc agcaatctca cgatctcggc ttcttgatct ccttcaagtg tcttgatgat
     4021 cgccaggatg agctggtcgg tctgggttag caagtcttct atcccaatgc tggaattggg
     4081 cttgaagcag tcgtcaattg tccggatcaa tgaccagatc tggtagagca aggttgtcgg
     4141 ctcgtcgaat aactccctca ggaaagccgc ctcttcgtaa cgcccgg
//
LOCUS       DBNHOH010000607         2058 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_998425, whole genome shotgun sequence.
ACCESSION   DBNHOH010000607 DBNHOH010000000
VERSION     DBNHOH010000607.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_998425"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="AC16V7_10590"
     CDS             complement(<1..838)
                     /locus_tag="AC16V7_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011394217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HIR6257609.1"
                     /translation="MKRRRMMRFWVAGVMILTLSLISVLSAVSQPAGPGLKLILIDGL
                     SRSEIKQLARMGLDIASVREGPVAEGPRGIPMQTYSVEVVVTSIDAAKLGSQGFSWSE
                     VPGKGPQKKIGEPYEVYHSFDEPINGIKDELRKIAATYPHIAQLKTIGKSIQNRPMLA
                     MRLTNEKVGKKTGKPQVLFLATHHAREWVATQMGMRLIKYLTANYGSEARVTNLLDSV
                     EVWVIPVANPDGYQYTFTNERLWRKNLRDNNGDGQITIADGVDLNRNFSNHWGLDNEG
                     SSP"
     gene            1467..1706
                     /locus_tag="AC16V7_10595"
     CDS             1467..1706
                     /locus_tag="AC16V7_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257610.1"
                     /translation="MISTVTASTVSTITTATLVGPVALIGVLVLLVLLVQKELASAAT
                     GNRFRTLVRALDIGIVPLLIAFGLAVISRAVEILR"
     gene            complement(1822..>2058)
                     /locus_tag="AC16V7_10600"
     CDS             complement(1822..>2058)
                     /locus_tag="AC16V7_10600"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HIR6257611.1"
                     /translation="RGLMQRAAHAVMGAFGLLYVSLLFAVLYIGYRSLVNFGFVFCVG
                     LAFSLVARRTRSIWGVSLAHGLTNIALFIIFPNL"
BASE COUNT          446 a          524 c          573 g          515 t
ORIGIN      
        1 cagggcttga accctcattg tcgagccccc agtggttgga gaagttgcgg ttgaggtcca
       61 ccccgtcggc gatggtgatc tgcccgtcgc cgttgttatc acgcaggttc ttgcgccaca
      121 gcctctcatt ggtgaaggtg tactggtagc cgtctgggtt ggccaccggt atgacccaca
      181 cctcaacgct gtccagcagg ttcgtcacgc gcgcctcgga gccgtagttg gcggtcaggt
      241 acttgatcag gcgcatgccc atttgggtgg ctacccactc gcgggcgtgg tgagtggcaa
      301 ggaaaagcac ctggggtttg ccggtcttct taccaacttt ctcgttggtg agacgcatag
      361 ccagcatggg ccgattttgg atgctctttc cgatcgtctt gagctgggca atatgggggt
      421 aagtggcggc aatcttacga agttcgtctt tgatgccgtt gatcggctcg tcaaagctat
      481 ggtatacctc gtagggttcg ccgatcttct tttgcggccc cttgcctggt acctcggacc
      541 aactgaaccc ctggctgcct aacttcgccg cgtcgatgct ggttacgacc acctccacgc
      601 tataggtctg catggggatg ccgcgcgggc cttccgctac tggcccctca cgcaccgaag
      661 cgatatctaa gcccatacgg gcaagttgct tgatttcaga ccgagacaga ccatcaatca
      721 agattagctt aagaccgggt cccgcaggtt gcgataccgc tgacagtacc gagattaagc
      781 ttagggtcag gatcataact cctgccaccc aaaaacgcat catcctacgt cttttcatcg
      841 catagcctcc tttgattgaa ttttttccgg ttaataaact tgattatgaa acatctgagc
      901 agggacgttg accctcatat cctgatgaac atggatatct taaaaagcgc tccaacccca
      961 cctcctttca gattgagcat tgctgattgg ctactttggc ttcgaatggt gctattccat
     1021 tacatcaaaa ggtgaagcca aaattaccaa tttggcgtcg tagggcgatt ttcccactct
     1081 tccaccttta tattatacag ttaaaatgat gtgaataatg cgtaattttt ttgtttttta
     1141 tggatttact cttcttccac aagaaaccag ctgtctgagg aaaaaatggt tgattggtgt
     1201 gggtactgcg caagaatgag tttgacccag aagctaggta ccctgttgac gtagacagcc
     1261 ggttcctccg tacaacccga tcagcttgtg attgcatctt tagttctggc gccggaaaga
     1321 tccaagcgcc agacacattg acggaggtga cagccttgcc ctgttaaggc atgtctttaa
     1381 tgatgacaat ggtgtcaggt ttattgacga gggctttgtt cgataataga atcatctgtg
     1441 ggagaggaag gggttgggga ggacttgtga tttctacggt tacggcatcc acagtttcca
     1501 ctattactac ggccacatta gttgggccgg ttgccttgat tggcgtcctg gtgctgctgg
     1561 tcttgctggt gcagaaggag ctagccagcg ctgcgacagg caaccgcttc cggacactgg
     1621 tcagggcgct cgacatcggc attgtgccgc tgctgatcgc atttggcctg gctgtgatct
     1681 cccgggcagt cgagatattg cgctgaggtt gcttggtgct caaatgaaaa ggctggctca
     1741 caagccaacc tttttgtatt ccttcaccgc gagaagtttt ccgggaggtg tattaaaggg
     1801 gtgaattcct cgccagcggg ttcacaggtt tgggaagatg atgaacaggg cgatattggt
     1861 caacccgtgg gcgagcgaga cgccccaaat ggagcgggtg cgcctggcga ccaggctgaa
     1921 ggccaggccg acacagaaga cgaagccgaa atttaccaag gagcggtagc cgatatacag
     1981 gacggcgaac agcagcgaga catagagcag gccgaaagcg cccatcacgg cgtgcgcggc
     2041 gcgctgcatc aggccgcg
//
LOCUS       DBNHOH010000608         4607 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1510522, whole genome shotgun sequence.
ACCESSION   DBNHOH010000608 DBNHOH010000000
VERSION     DBNHOH010000608.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1510522"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="AC16V7_10605"
     CDS             <1..917
                     /locus_tag="AC16V7_10605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6257612.1"
                     /translation="ARDLKAEWNVPIVHMFHTLGIMKQRVARVPDEAEGDYRLKGESD
                     VLQLADRIVASTLAELAQLQWLYKANVRKVVIIPPGVDLTHFYPIPTDEAKEYIGVLP
                     CEQVVLFVGRIEPLKGIDTLIEAIAILRQRNVTVCLSVIGGDADVSQDELSDEMARLQ
                     VLREKYDLQDLVTFLGRRGQDTLPYYYSASEAVVVPSHYESFGMVALEAMACGTPVVA
                     SQVGGLAYLVQDGVTGFTVPVDEPQALADCLIRLLQDASLRKRMGEQAAAFALDYDWD
                     KIAARIVKLYEEISREDSRLSEADGTPH"
     gene            1141..2532
                     /locus_tag="AC16V7_10610"
     CDS             1141..2532
                     /locus_tag="AC16V7_10610"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HIR6257613.1"
                     /translation="MTTILIQHANTLVTMDDHRREIPDGGLFIRDGFIEQVGETSQLP
                     ETADDILNLRDHIVLPGLVNTHHHFYQTLTRVIPAAQNANLFNWLKTLYPIWAKLTPD
                     DIYVSTQTALAELALSGCTTASDHLYIFPNGSRLDDEIHAAQEIGLRLHASRGSMSLG
                     ESKGGLPPDSVVEEEDFILRDSIRLIETYHDSKPGAMTQIVLAPCSPFSVTGDLMRQS
                     AVLARQYGVQLHTHLAETQDEEAFCQEMFGYRPVGYMQHLDWVGTDVWFAHSVHVSQE
                     EVGLYSRTGCGVAHCPTSNMRLASGFAPIYEMLVARVKVGLGVDGSASNDGSHLLEEV
                     RQAMLMARLRAALGGASLSSEGAPPILTARQALELGTRGGAAVLGRRDLGSLEPGKCA
                     DFFAINLNRLDYAGALHDPVAAVVFCTPQHVDYTMVGGKLVVKEGQLATLDLPMLVER
                     HNQAAHRLVYKPD"
     gene            2581..4395
                     /gene="ade"
                     /locus_tag="AC16V7_10615"
     CDS             2581..4395
                     /gene="ade"
                     /locus_tag="AC16V7_10615"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HIR6257614.1"
                     /translation="MKKSPFNRVDTRALVDVAMGRVPADLVIRNGRWVCVQSGEIIPN
                     TDIAVKGERIAYVGPDAGHTIGKETKVIDAQGYYLVPGLLDGHMHVESGMLTVTEFVR
                     AVIPHGTTGMFVDPHEIANVFGLKGVRLMVDEAVVQPIHVWVQMPSCVPSAPGLETPG
                     ASIGPEEVAEAMTWEGIIGLGEMMNFPGVFFSDEKMHAEMAETRQAGKVIGGHYASPD
                     LGVPFHGYVAGGPEDDHEGTRLEDAVARVRQGMKPMLRFGSAWHDVASQVRAITEMGL
                     DARHFILCTDDSHSETLVKEGHVNRAVKIAIANGVPPITAIQMSTLNTAEHFGLARDV
                     GLIAPGRFADVLLVKDLPEMVPSQVISRGKLIAENGQLLIDLPAYPYPEWAVHSVRQA
                     HPLAAEDFRLSLPDGFSSNLKTVSANVIGVIENQAPTRHLKIDVPALEGEVNVDISRD
                     LAKIALVERHEGSGRVQVALVHGFGFDTSCAIGSTVAHDSHHMIVVGTDEADMAMAAN
                     ELAKVGGGQVVVKEGKIIGMVELPIAGLMSNEQAEVVAEKALTVLEGFHTCGCKLNNP
                     NMQLSLLALVVIPELRISDLGLVDVTRFEFVPVFEERE"
     gene            4392..>4607
                     /locus_tag="AC16V7_10620"
     CDS             4392..>4607
                     /locus_tag="AC16V7_10620"
                     /inference="COORDINATES: protein motif:HMM:NF012610.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR6257615.1"
                     /translation="MIHTSNTSQRLHESLGEIIAGTHNGGRLPSEPELARRLGVSRAT
                     LREAMRTFETQGLIRRRQGSGTYVVHPT"
BASE COUNT         1036 a         1094 c         1322 g         1155 t
ORIGIN      
        1 cagcccgcga cctgaaggcg gagtggaatg ttcccatcgt gcatatgttt cacacgcttg
       61 gcataatgaa gcaacgggtg gcgcgcgtgc ctgatgaagc agaaggggac taccgtttga
      121 agggtgaaag tgatgtgctg cagcttgcgg atcgaattgt ggcttctact ttagcggagt
      181 tggctcaact gcaatggctg tacaaagcga atgtgcgcaa ggtggttatt attccaccag
      241 gcgtggattt gacccatttt taccctattc caaccgatga ggctaaagag tacatcggtg
      301 tgctgccttg tgaacaggta gtgctctttg tggggcggat cgagccattg aaaggtattg
      361 acacgttgat tgaagcgatt gcgatccttc gacagcgaaa tgtgacggtc tgcctctcag
      421 tgatcggtgg agatgcggat gtcagtcaag atgaattgag cgatgaaatg gctcgcctgc
      481 aagttcttcg cgagaaatac gacttgcaag atttggtcac ttttttaggg cgtcgggggc
      541 aagatacgct accctattat tactcggctt ctgaggcggt ggttgtccca tctcactatg
      601 aatcgtttgg catggttgcc ctggaagcca tggcatgcgg tacaccagta gtggcctcgc
      661 aggtgggtgg gctcgcttac ctggtgcaag atggggtcac aggattcacc gtcccagtag
      721 atgagcccca ggcacttgca gactgtttaa taaggctgtt gcaggatgcc tctttacgca
      781 agcgtatggg ggagcaagca gctgcatttg cactcgacta tgattgggat aagattgcag
      841 cgaggattgt caagctctat gaggagattt cacgggaaga tagtcgattg tctgaagccg
      901 acggaactcc tcattaagtt ttgatttcca aacaagccat ataatccata tgcgggtcgc
      961 tggagacgcg atagcggatt gctcggatat tttcagacca tttgtcggct agcatagccg
     1021 aggggggaaa tattattttc ggcaatcgct caagttacaa atattacttg tcagacaatt
     1081 gacgatttta cttttgtttg ttaaaataaa tttgtcagac aagttggctt gggtcatacg
     1141 atgactacta ttcttattca acacgccaat acgcttgtga ctatggatga ccatcgccgt
     1201 gagataccag acggtggtct ttttattcgg gatggtttca tcgaacaggt gggagagaca
     1261 agccagcttc cggagactgc cgatgatatc cttaatttac gcgatcacat cgttctaccg
     1321 ggtctggtca atacccatca ccatttttat caaacgctga cacgggtcat tccagccgcg
     1381 caaaatgcca acctgttcaa ctggctcaag accctatatc ccatttgggc aaagttgacg
     1441 cccgatgata tttatgtctc aacccaaact gctctggcag agttagcgct ttcaggttgc
     1501 actacggcct ctgatcactt gtacattttc ccaaatggct cgcgcctgga tgatgaaatc
     1561 catgctgcgc aggagattgg tttgcgcctg catgcttcac gcggctcgat gagcctcggt
     1621 gagagcaagg gaggcttgcc acctgattct gtcgtggagg aagaggattt catcctgcgc
     1681 gattctatcc gcttgatcga aacttatcac gattcaaaac ctggcgcgat gacccaaatt
     1741 gttttggcgc cttgctcgcc attctctgtc acgggtgatc tgatgcgcca gagcgcagtc
     1801 ctggcgcgcc agtatggtgt tcaattacat actcatctgg cggagacgca ggatgaagag
     1861 gcgttttgcc aagagatgtt tggttaccgt cccgttggtt acatgcagca cctggattgg
     1921 gttgggacgg atgtctggtt tgcccactct gttcacgtta gccaggaaga ggtgggtctg
     1981 tactcacgca ctggctgcgg cgtggcccac tgcccaacct caaatatgcg tctggcttct
     2041 ggtttcgcgc ccatttatga gatgctagtc gcgcgtgtaa aggtggggtt gggtgtagat
     2101 ggctctgcta gcaacgatgg ctcccacctg cttgaggaag ttcggcaagc gatgttaatg
     2161 gctcgtttaa gagctgcgtt aggaggtgca tcactttcaa gcgaaggcgc accaccaata
     2221 ttaaccgcca ggcaggcttt ggagctcggt actcgtggag gagctgccgt gcttggcagg
     2281 cgcgatttgg gctcattaga accgggtaaa tgcgccgatt tctttgccat taacctcaat
     2341 cgcttggact atgctggcgc gctgcacgat ccggtcgcag ccgtggtttt ttgcacacct
     2401 caacacgtag attacaccat ggtaggtggc aagctggtcg tcaaagaggg ccagttagct
     2461 acattggatc tgcctatgct ggtggagaga cataatcagg ctgctcacag gttggtttac
     2521 aagcctgatt aattaccagc gcatattggg aagcgagcaa ttaccggaat tggaggaaag
     2581 atgaaaaaat cccctttcaa tcgtgttgat actagggcgc tggtggatgt tgccatgggg
     2641 cgagtgccag ccgacctggt gatccgaaat gggcgttggg tatgtgtgca atccggtgag
     2701 atcattccca atacagatat tgctgtgaaa ggtgaacgga tcgcttacgt aggacctgac
     2761 gctggtcata caatcggcaa ggaaaccaaa gtcattgatg cccaaggata ctatcttgtc
     2821 ccaggattat tagatgggca tatgcatgtt gaatcaggca tgctgaccgt cactgaattt
     2881 gtgcgggcgg tcattccgca tggcacgacc ggtatgttcg tggaccccca tgaaatcgcc
     2941 aacgtctttg gtctgaaagg cgtgcggttg atggtggatg aagccgttgt gcaacccatc
     3001 cacgtttggg tgcaaatgcc ttcctgcgtc ccctccgctc cgggcctgga gacgcctggg
     3061 gcgagtatag gaccagaaga ggtggcagag gccatgacct gggagggaat catcggcttg
     3121 ggagagatga tgaactttcc cggagttttc ttcagcgatg agaaaatgca tgccgagatg
     3181 gcagagacgc gtcaagccgg aaaggtgatc ggggggcatt atgcctcgcc agacctgggg
     3241 gtgcctttcc atgggtatgt ggctggtggc ccagaggatg accatgaggg cacacgcctg
     3301 gaagatgctg ttgcccgtgt gcgccaaggc atgaagccca tgcttcgctt cggatctgca
     3361 tggcacgatg tagcctccca ggttcgggcg atcactgaga tgggtttgga tgccaggcac
     3421 ttcatactgt gtactgacga ctcgcattct gagacactgg taaaagaagg gcatgttaat
     3481 cgtgccgtga agattgctat cgccaatggt gtgcccccga ttacagccat ccagatgagc
     3541 accttaaata cagcagaaca cttcgggttg gcgcgggatg tcggattgat cgctcccggc
     3601 cggtttgctg atgtattgct ggttaaggat ctccctgaaa tggtgccctc ccaggtcatc
     3661 tcacgcggga agttaatagc ggagaatggt caattgctga tcgacctgcc tgcataccca
     3721 tatccagaat gggcggttca ttccgtccga caagcccacc cgctggctgc agaagacttc
     3781 cgattgtcct tgccggatgg cttctcatcc aacctcaaaa ctgtgagcgc caatgtgatt
     3841 ggagtaattg aaaaccaagc tccaacccgc catttgaaga tagatgttcc tgctctagag
     3901 ggcgaggtta atgtggatat ctcgcgcgat ttggcgaaaa ttgctttggt ggaacgccac
     3961 gagggctcgg ggagggtgca ggtcgccctt gtacatgggt ttggctttga tacctcctgc
     4021 gcgataggtt ccacggtcgc ccatgatagc caccatatga ttgttgtggg cacagatgag
     4081 gccgatatgg ctatggcagc caatgagctg gccaaggttg ggggtggtca agtggttgta
     4141 aaagagggca agatcatagg catggttgag ttacccatcg ccgggttgat gtcgaacgag
     4201 caggcggagg tcgttgccga aaaagcatta acagttttag agggtttcca cacctgcggt
     4261 tgcaagctca acaacccaaa tatgcagctc agcctactag cactggtggt gatacccgaa
     4321 ctgcgtatct ctgacttggg tctggttgat gtgactcgtt ttgagtttgt ccccgttttt
     4381 gaggagcgcg aatgatccat acctccaaca cctctcaaag attgcacgaa agccttggtg
     4441 agatcattgc tggcacccat aacggtggca gactcccctc ggagcccgaa ctagcaaggc
     4501 gcttgggtgt ttccagagcc actcttcgcg aggccatgcg cacctttgag acccagggat
     4561 tgatccgccg ccgtcagggc tctggcacct atgttgtgca tcctacc
//
LOCUS       DBNHOH010000609         6900 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1062567, whole genome shotgun sequence.
ACCESSION   DBNHOH010000609 DBNHOH010000000
VERSION     DBNHOH010000609.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6900)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6900)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6900
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1062567"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="AC16V7_10625"
     CDS             <1..612
                     /locus_tag="AC16V7_10625"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HIR6257616.1"
                     /translation="TGIEAGPDGEAWYELTSEIDAHLKYFVPTAHLRPISDEEISPIS
                     PDVPPEQKRIEVSIGRQTLTAYEGDKVMLSTKVSTGIPSARRSPEDLPTATPTGRFRI
                     YSKMPNKHMGFITGNPDALRNGGFSLPGVPWTCFFAFPGGYAFHGTYWHNNFGIQMSH
                     GCVNLRNEDALWLFRWSTPVFKTPIESHTDWERRGNGTTVEIG"
     gene            609..1655
                     /locus_tag="AC16V7_10630"
     CDS             609..1655
                     /locus_tag="AC16V7_10630"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR6257617.1"
                     /translation="MITRRSFLKLGGAAAASLFTHPSSPYLLLPDDEPPRLAGKARVA
                     TGLVYRYSQASFQSERIGNLKRDQIVDIHEETLSPDGPNYNPRWYRLSKGYAHSGRLQ
                     RLDGAHFHRDVLEAVPETGQLGEISVPFTQSYRKMPGNQWAPLYRLYYQSIHWITGLD
                     SGPDGRTWYRLTDDLLHVHHYVPSFHVRPVASDELSPISPRVPPDDKRIAVSIADQTL
                     TAYENDQIVFTSKVSTGIPSEPPPGMLPTETPRGRFYVQTKMPSRHMGDGNLTDDIEA
                     YELPGVPWVCFFHKDGYAFHGTYWHDNFGRMMSHGCVNMRTQDALWLYRWTTPVAPAK
                     NWFTRQMGTRVDIT"
     gene            complement(1652..2485)
                     /locus_tag="AC16V7_10635"
     CDS             complement(1652..2485)
                     /locus_tag="AC16V7_10635"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006826924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6257618.1"
                     /translation="MAPEWIVETRDLTRIYGDGEEIHALDGVNLQIAPGEVVAVMGPS
                     GSGKSTLLNMIGALDKPTRGQVFVNGQDLAKIRNKDAFRAKTVGFMFQLHNLLPTMTA
                     RENVEVPMMGHRGLRTRHKRAKELLELVGLPDRMNHLPNQLSGGQRQRVAVARALAND
                     PPLVLADEPTGSLDTVAGMDLLKLLHELNHSQGTTFLVVTHDIAVARQTHRVVVMADG
                     KIVREDLIGSPLEEDLKMWRHSGLGRRILEEEMDVIQKLGIDESQARVLREIFTSANS
                     D"
     gene            complement(2497..3684)
                     /locus_tag="AC16V7_10640"
     CDS             complement(2497..3684)
                     /locus_tag="AC16V7_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR6257619.1"
                     /translation="MQKKSLSILLTSVLIGLILVSGCDALGQRATPTPEAVVEEDFVP
                     LVNATGVVVPARFTTLSMSASGVVAEVLVSENDVVSAGQPLVRLEGKEDLEAAIAAAR
                     FEVAAAQHDLDLLYKDLDLRLANAQQVVVDAKQALRDAERRQYNVERAAKQVDIDQAR
                     ANLVLARDKLEKAVEDFEPYENKPEDNLTRAALLSAKASAEEQYDSSVRLLNNLLAGA
                     NDLDVAEANAEAEVAKAQLAVAEREVELLKVGPDPEDVKLAEGRLANAKVQLTSAEAA
                     LGDLELLAPFNGTVTELYIKQSEWISFGQSVLLLADLDHLRVETTDLNEIDVARIDEG
                     STTLITFDALPDVSTQGQVIRIAPKASAGSGVNYTVVVEMDEIPKSLRWGMTAFVDIE
                     VQE"
     gene            complement(3699..5945)
                     /locus_tag="AC16V7_10645"
     CDS             complement(3699..5945)
                     /locus_tag="AC16V7_10645"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6257620.1"
                     /translation="MFLKNLFRRKIRTALTVLGISIGVAAILGLGAMASGLEAGYGSM
                     LSGSKADLILSQPDSFDMSYSVVGENIGVQLAAMPEIHALSSMLEGFVQAENEPYFFV
                     FGYPEDSFILDRFQIVDGAALSSREARSAHGKPILLGSAAAEVLDKAVGDSMRLTGSV
                     YRIIGIYQTGDAFEDSGAILRMQDAQELLGKPRQVSVFYIQLKDPALRERFIKRVERQ
                     MPELELSGVQEFQDKQIMSDMFAGYVWVIGGLAILIGGVGMMNAQLMSVFERTREIGV
                     LRSVGWSRSRVLWMILAESMFVCLAGGVLGLGLGYLLLDVISRNTVIFGASTASIGAS
                     SLQQAFIVVVVMGLVAGLYPAWRASRLQPVEALRYEGGAASKARRLPVGGMAVQSLRQ
                     RVTRTLLTVMVIGITVGAIAALEGLLLSMKADMTAMFSSGGNQIMIRQADVSDTSLSA
                     IDERIGDKIAALSEVETVSGVMIAAVVLPDTGGFFIMLGYGPNEQAIRRFRIVDGEPL
                     KSNHQIILGKSMADAMKKNVGDTIDLSGARFRVVGIYESNISWEELGGVASLRDTQLF
                     IGRPRKVTMFAVSVRDPSQAEEVVEKINATFPEVHASLSGEFADQMPDFEASEGMMSG
                     ISVMAIFIGGVGVLNTMLMAVFERTREIGVLRSLGWGRSAILSMILRESLLLGLLGGG
                     AGIVIAFCLVQLASIAPIVGDYFGVTWQWQIFARAIGIAIMLGLLGGLYPAYRATRLQ
                     PVEALRYE"
     gene            complement(5974..6600)
                     /locus_tag="AC16V7_10650"
     CDS             complement(5974..6600)
                     /locus_tag="AC16V7_10650"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6257621.1"
                     /translation="MQKFKKPPQNRRQRRVAARRAEILNAATRLFAEKGFHRTTTRDI
                     AAAADVSEGTLYNYFENKDDILFGIMSRLTESQDLNTSLAHAIPDDARGFLYDTLIQR
                     KQFVDQNSDMLQSVLSEILVNPELRQRYYRELILPSIELLEAHLQVRQDQGQIRPLNI
                     PMSVRFLVALTNGLFFLDVLGDPLVQSQWDELSQLITSIFFDGVDPRP"
BASE COUNT         1600 a         2134 c         1694 g         1472 t
ORIGIN      
        1 acaggcatcg aagccgggcc agatggtgag gcctggtatg aattgacatc cgagatcgac
       61 gcccatttga aatatttcgt gcccaccgca catctacgcc ccatctccga tgaggagatt
      121 tcccccatct cccctgacgt gcccccagaa cagaagcgca tcgaggtctc aattgggcgg
      181 cagaccctga ccgcctatga aggcgataaa gtgatgctct cgacaaaagt ctcgacgggc
      241 atccccagcg cccgccgctc cccggaagat ctgccgaccg caacccccac cggaagattc
      301 cgcatttact cgaaaatgcc caacaagcac atgggcttca tcaccggaaa tccggatgcg
      361 ctcagaaatg gcggcttctc gctgcctggc gtgccttgga cgtgcttctt cgcctttcca
      421 gggggttacg ccttccacgg cacctactgg cacaacaatt tcggtattca gatgagccac
      481 ggatgtgtca acctgcgcaa cgaagatgcc ttgtggctct tccgctggtc cacgcccgtg
      541 ttcaaaacac ctatcgaatc gcacaccgac tgggagcgcc gcggtaatgg tactacggta
      601 gagatcggat gatcacccgg cgaagcttcc taaagcttgg gggcgcagcg gcagcctcgc
      661 tcttcaccca cccatccagc ccctatcttt tgcttccaga tgatgaacca ccccgtcttg
      721 ctggcaaggc gcgcgtggct acgggactgg tctaccgcta tagccaagcc tcctttcaaa
      781 gcgaacgtat cggcaacctc aagcgcgatc agattgtcga tatccacgaa gagaccctct
      841 cccccgatgg tcccaactac aacccgcgct ggtaccgcct ctctaaaggt tacgcccaca
      901 gcggtcgcct gcagcgcctg gatggggctc attttcaccg cgacgttttg gaagccgtcc
      961 ccgaaaccgg ccaactgggc gagattagtg tgccctttac acaatcctat cgaaaaatgc
     1021 ccggcaacca gtgggcaccg ctttaccgcc tgtattacca atcgatccat tggataaccg
     1081 gcctggatag cggtccagat ggcagaacct ggtatcgact gacagatgac ctgcttcatg
     1141 ttcaccatta tgtcccgtct tttcacgtgc ggcctgttgc cagcgatgaa ctgagcccaa
     1201 tctcacctcg ggtgccccct gatgacaagc gcattgccgt ctccatcgca gaccaaaccc
     1261 tgaccgccta tgaaaacgac cagatcgttt tcaccagtaa agtctccacg ggcataccca
     1321 gtgagccgcc ccccggcatg ctgcctaccg aaacaccgcg cggcaggttt tacgtacaaa
     1381 caaaaatgcc atcccgccac atgggggatg gcaacctgac cgacgacatt gaagcctatg
     1441 agctaccggg tgtgccctgg gtgtgtttct ttcacaaaga cgggtacgcc ttccacggga
     1501 cctactggca cgataatttt gggcgcatga tgagccacgg ctgtgtcaat atgcgcacgc
     1561 aagacgcttt gtggctttat cgctggacaa cacccgttgc acccgcaaaa aattggttca
     1621 cccgccaaat gggcacacgg gtagatatca cctagtcact gttagcggaa gtgaaaattt
     1681 cccgcaggac ccgagcctgg ctttcgtcaa tccccaattt ctgtatcaca tccatctctt
     1741 cttcgagaat tcgcctgccc agccccgagt gacgccacat cttcaggtct tcctccaggg
     1801 gggagccaat caggtcctcg cgcacgatct tgccatccgc catgaccacc acgcggtgcg
     1861 tctggcgcgc cacagcgata tcatgagtca cgaccaggaa tgtggtaccc tggctgtgat
     1921 tcagctcgtg taataatttg agcagatcca tgcctgccac ggtatccagg ctgccagtag
     1981 gctcgtccgc gagcaccagg ggagggtcat ttgccagggc acgcgccacc gccacgcgct
     2041 gcctctgccc accggagagc tggttgggca ggtggttcat ccggtctgga agacctacca
     2101 gctccaagag ctctttggcg cgcttatggc gggtacgcag gccgcgatgc cccatcatgg
     2161 gcacttccac attctcgcgt gcagtcatcg tcggcaataa gttatgtaac tggaacatga
     2221 agccaaccgt cttagcgcgg aaagcatcct tattgcggat cttagccagg tcttgcccgt
     2281 tgacgaacac ctgcccccga gtcggtttat ccagcgcacc aatcatgttc agcaaggtgc
     2341 ttttgccgct gccgcttggt cccatcactg ctacaacctc gccgggcgct atctgcaaat
     2401 tcacgccatc cagcgcgtga atctcttcgc catcaccata tattcgcgtc aaatcacgag
     2461 tttctacaat ccattcaggt gccattgata aatcctctat tcctgaactt cgatatccac
     2521 aaacgccgtc atgccccagc gtaaactttt cgggatctca tccatctcaa ccaccacagt
     2581 atagttaacc ccagaccctg cggaggcctt gggtgcaata cggatgacct gcccttgggt
     2641 agacacatcc ggcagagcat caaatgtaat cagggttgtg ctgccctcat caatacgggc
     2701 gacatcaatt tcatttaggt cggtggtctc cacgcgcaag tggtcgaggt ccgccaataa
     2761 caacacagac tgaccgaatg agatccattc actttgcttg atatatagct cggtgaccgt
     2821 accattaaat ggagccagca gttccagatc gccaagagcc gcttcagcac tggtgagctg
     2881 gactttagcg ttcgccagtc gcccttcggc gagcttgaca tcctccggat ctgggcctac
     2941 cttgagcaac tcaacctcgc gctccgcaac agccaattgc gcctttgcaa cttccgcttc
     3001 tgcattcgcc tcggcgacat ccagatcatt cgcgccagcc agcaggttat tcagcaggcg
     3061 cacagacgaa tcatattgct cctccgcgga cgcttttgcg ctcaacaacg cggcccgggt
     3121 caggttatcc tcaggtttat tctcgtatgg ctcgaaatct tccactgcct tctccagctt
     3181 gtcgcgagca agcaccagat tggcgcgcgc ctggtcgata tctacctgct tggcggcacg
     3241 ctcgacgtta tactggcggc gttctgcatc ccgcaatgct tgcttggcat ctacgacgac
     3301 ctgctgggcg ttggcaaggc gcaaatccag gtctttatac agtaagtcaa ggtcgtgctg
     3361 ggcagctgcc acttcgaaac gagcggcagc tatggcagcc tccaaatcct ctttaccttc
     3421 aaggcgcacc aatggctgcc cagccgagac aacatcattt tcgctcacca agacctcggc
     3481 cacaactccg ctggcagaca tactcaaggt agtgaatcgc gccggtacga ctaccccagt
     3541 agcgttaacc agtggaacga agtcctcctc aacaaccgct tccggtgtag gcgtagcgcg
     3601 ctgccccagg gcatcacacc ccgaaaccag aataagtccg ataagtacag aagtcaacaa
     3661 gatagaaagc gatttctttt gcattggaac tctccaaatc actcgtatct aagcgcctcc
     3721 acaggctgca atcgtgtggc gcgataggcg gggtaaagcc cgcccagtaa accaagcatg
     3781 atagcaatcc cgatggcgcg ggcgaagatt tgccactgcc aagtgacacc gaagtaatca
     3841 cccactattg gtgcgattga agccaattgg accaggcaaa aagcgatgac tatcccagcc
     3901 cctccgccca gcaagcccaa caagagcgat tcgcgcagga tcatgctcaa tatggcgctg
     3961 cgtccccaac ctagcgaccg caatacgcca atctcgcgcg tgcgctcaaa gactgccatc
     4021 agcatggtat tcagcacacc caccccaccg atgaagatcg ccatgaccga gatgccgctc
     4081 atcatacctt ccgaagcttc gaaatcgggc atctgatcgg caaattcgcc actcagcgag
     4141 gcgtgcactt cagggaatgt tgcattgatc ttctcaacca cttcctccgc ctgagacggg
     4201 tcgcgtactg aaacagcaaa catggtaact ttgcgaggcc gaccaatgaa taactgggta
     4261 tcccgcaagg aagcgacgcc gcctaattct tcccaggaga tgttgctctc atatatcccc
     4321 acaactcgga agcgcgcgcc gcttaaatca atggtatcac cgacgttttt tttcatggca
     4381 tcggccatag acttaccgag gatgatttgg tggttgcttt ttagtggctc cccatctacg
     4441 atgcggaaac ggcgaattgc ctgctcatta ggtccatagc ccagcatgat gaagaaacca
     4501 cccgtgtcgg gcaatacgac cgctgcgatc ataactccgc tcacggtctc gacttcagac
     4561 agcgccgcaa ttttatcgcc aatgcgctca tcgatggcac taagagacgt atcgctgaca
     4621 tctgcttggc ggatcatgat ctggttgccg ccgctagaaa acatggcggt catatctgcc
     4681 ttcatgctga ggagcaagcc ctccagcgcg gcgatggcgc ccaccgtgat gccaatcacc
     4741 atgacagtca gcaatgttcg ggttacccgt tgccgcaagc tttgcaccgc catcccgccc
     4801 acgggcaaac ggcgtgcttt actggcagca ccgccttcat agcgcagcgc ttccacaggc
     4861 tgcaagcggg atgcccgcca ggctggatag agaccagcta ccaatcccat cacaaccacg
     4921 acgatgaaag cctgctgcaa cgagctagcg ccgatggatg ccgtacttgc tccaaaaata
     4981 actgtgtttc ttgagatgac gtccaacagc aaataaccca ggcccagtcc caacactccg
     5041 cctgccaggc agacaaacat cgattcagcc aggatcatcc acagcacgcg gctgcggctc
     5101 cagcccacag agcgcagcac gccgatctca cgcgtgcgct cgaacaccga catcaactgg
     5161 gcgttcatca tgcccactcc accaatcagg atcgccagac cgccgatcac ccacacataa
     5221 cccgcaaaca tgtccgacat gatctgctta tcttggaact cctgcacccc gctcaactcc
     5281 agctcaggca tctggcgctc gacgcgcttg ataaagcgct cccgcaaggc gggatccttc
     5341 agctgtatat agaacacact gacctggcga ggcttcccca atagctcctg ggcgtcctgc
     5401 atgcgcaaaa tagcaccaga gtcttcaaag gcatcacctg tctggtaaat gccgataatc
     5461 cgataaacgc taccggtcag gcgcatgctg tcgccaactg ctttatccag cacctcagcc
     5521 gccgccgaac caagcaggat cggcttgcca tgggcactcc gggcttctcg ggacgataac
     5581 gccgccccat cgacgatctg gaagcggtcc aggatgaaac tgtcctctgg atacccaaaa
     5641 acaaagaagt atggctcgtt ctctgcctgg acaaatccct ccagcatgct gctcaaggca
     5701 tggatttccg gcattgcagc aagctgcacg ccaatatttt cacccaccac gctgtaactc
     5761 atatcaaacg agtccggctg gctgaggatc aagtccgcct tgctgccaga gagcattgag
     5821 ccataaccag cttccagccc acttgccatg gcacccaaac cgagaatagc agccacaccg
     5881 atgctgatac ccagcacggt aagtgcggtg cgtatttttc ggcggaaaag gttcttcaaa
     5941 aacataacga tccttttcgg atatcaggtt gccctatggg cgcgggtcta cgccgtcaaa
     6001 gaaaatactg gtgatcaatt gcgagagttc atcccactga gactgcacca gtgggtcgcc
     6061 tagcacatcc aggaagaaaa gcccgttggt cagggcaacc aagaagcgca ccgacatggg
     6121 aatgttcagc gggcggattt gcccctgatc ctggcgcact tgcaggtgcg cttcgagcag
     6181 ttcaatgctg ggcagaatta gctcccggta atagcgctgg cgcagctcag gattgaccaa
     6241 tatctcggac aagacggact gcagcatatc gctattctgg tcaacgaact gcttacgctg
     6301 gatgagcgtg tcatacagga aacccctggc gtcgtctggt atagcatggg ccaggctggt
     6361 gttcagatcc tgactctccg tcaggcggga cataatgccg aagagtatat catccttgtt
     6421 ttcgaaataa ttgtatagcg tgccttccga gacatccgcc gcggcagcga tatcgcgggt
     6481 ggtggtgcgg tggaatccct tctcggcaaa cagacgggta gccgcattca gtatttctgc
     6541 ccggcgggcg gctacgcgcc gctggcggcg gttttgggga ggttttttaa atttctgcac
     6601 aggcgcgcct ccaaaatgag tgtttgctca caaaataagt gagcgttcat tcatatattg
     6661 agtatacgct cattttattc tcaaataact aacttgtcaa gggaggcgac ggaattgcct
     6721 cacgattttg ctcccgctgc cgctttcctc gaagaaagcc aatctcgctc atcatattgg
     6781 ccggctggta gaacttgact attcatacgt ggacaagctg gtgcttgttt acgaggataa
     6841 tccccctata ttcttaaaca tcgcctgtga tcaagttttc actggcgttt gtatcatcaa
//
LOCUS       DBNHOH010000610         2862 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_100160, whole genome shotgun sequence.
ACCESSION   DBNHOH010000610 DBNHOH010000000
VERSION     DBNHOH010000610.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_100160"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2862
                     /locus_tag="AC16V7_10655"
     CDS             <1..>2862
                     /locus_tag="AC16V7_10655"
                     /inference="COORDINATES: protein motif:HMM:NF024299.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6257622.1"
                     /translation="YDSAGLVEDAYQAARAALLLAPGDVENLNWFANQVLNLKGRPGG
                     EFPDAHNEAIESLERATQIAPQRGDLWLRLGGLQLQSGNRAGAVDALMYLADPESPVS
                     ESSANNLYQAAQWLNELGKADSAVICLERALQSGLEAHTPAGPSLLDMLTSLCAAHRQ
                     VGDLPAALQALDQAIAINPDEPELYLQKADILLGMSQTDGDANDSQAFAEQGVACLEA
                     AMKLNPHNPDLHYRAALTHRAAGELHLALVHATEMVDISASLSQAMRARSLAADLSLA
                     MLQPVQARTFLEANLPPSPPNEMVEDNVLLDYYTMRAELALEAGDERGAVEELVHILE
                     VAPDDPRLLAIQARVILQRDDPQAASSMLENVLKGMGDPSKARLALLRSAAQAALELG
                     QWEVAIQLCQQAISTAPLEPRSHLDLGRTLVLRAEFSTMCQALGAIGRAGDQQAFSDE
                     IQPTVETALQKTEEQIKAWEADGSIKLANSQNGETSGRIKIKWVDPEAQIHRWRTRGQ
                     AIFQPGSEAVKALAALPPNADDAAARIACLREMGDLTTAGLVARDYPQHPLVLLQLAL
                     TLLDEKPRQAMAAIHAAADSLSQATANRRFRSAFSLKEISPLVYALLANLFHRDGNRV
                     SDRDNALQAILNALSIWPDEPRWHVLAAEIYLAGDPMDSQGDLEAAINHLERATKLDP
                     EYTPSLIVLGQMYLREGSIQRAMQTFEQAAQAAPGEAEPWMWLAQACRSMGKMEQAAA
                     HAERAVTLAPNYIKPLILRGEIALQDENPRGALSRAEAALRIQPDDPDALLLQVRALN
                     ALDRYEEALGTLEKALSLASDPLPLSMERVRLLRRAQGNEAALHAIQSLADHYPDEPR
                     VMTLQAEVLEAAGDSEAAIRAAQRALRGQAGAPVISKVDQASLHFMLGSLLRSTGQLD
                     QSLHHLSESIHLAPDQVNGYLEMGHVHLERREHTQALSA"
BASE COUNT          650 a          818 c          794 g          600 t
ORIGIN      
        1 atacgattcc gctggactgg tggaagatgc gtatcaggcg gcacgggctg ccttattgct
       61 tgccccaggc gatgtcgaga atctcaactg gtttgctaac caggtactaa atctgaaggg
      121 gcgaccaggg ggtgaattcc cggatgccca taacgaagct attgagtcgc tagagcgagc
      181 tacccaaatc gctcctcagc ggggcgactt gtggttgaga ttgggaggct tgcaactcca
      241 atctggcaat cgcgcgggtg ctgtggacgc cttaatgtat ttggctgacc cagaaagccc
      301 cgtctctgaa agctctgcaa ataacctcta ccaggctgcg caatggttaa atgagctggg
      361 caaggcagat tcggcagtga tctgtttgga gcgcgccctt caaagcggct tggaggctca
      421 tacgccagct ggacctagct tgctcgatat gctcacatct ctttgtgcgg ctcaccgcca
      481 ggttggcgat ttgccggcgg cattacaggc tctggatcag gccattgcca tcaatccgga
      541 tgagccagag ctgtacttgc aaaaggcaga cattctactg ggtatgagcc agactgatgg
      601 cgacgcaaat gatagtcaag cattcgcaga gcaaggggtt gcctgtctcg aagctgccat
      661 gaaactaaat ccccataatc ccgatctaca ctatcgcgcc gctctgacac accgggctgc
      721 gggagagtta catttggctc tggtgcacgc aaccgagatg gtcgatatca gtgcttccct
      781 atcccaggcc atgcgagctc gctcactcgc ggccgatctc tcactggcta tgctgcagcc
      841 tgttcaggcg cgcacatttt tagaggcgaa tttgccgccc agccctccga atgagatggt
      901 tgaagataat gtgctactcg attactatac gatgcgcgcc gagttggctc tggaagcagg
      961 tgacgagcgg ggtgccgttg aggagctggt acatatactc gaggttgctc ccgacgatcc
     1021 gcgtctgctg gctattcagg cacgcgtcat cctccagcgc gatgatcctc aggctgcttc
     1081 gagcatgtta gagaatgtac ttaaaggcat gggtgaccct tcaaaagccc gcttagcctt
     1141 gcttcgctcg gcagcacaag ctgcgcttga gctgggtcaa tgggaagtgg ctatccagct
     1201 ttgccagcaa gcgatcagca ctgcaccatt ggagcctcgt tcgcaccttg acctcggtcg
     1261 cactttggtg cttcgggcag aattctccac aatgtgccaa gcacttggag ccatagggcg
     1321 cgcaggggac cagcaagcat tttcggatga aattcagcct accgttgaga cggctctaca
     1381 aaagaccgag gaacagatca aagcctggga ggctgatggc agcataaaat tggcaaattc
     1441 ccaaaacggc gagacgtccg gccggataaa aattaaatgg gtagatcccg aggcgcaaat
     1501 ccatcgctgg cggacgcgtg ggcaggcaat cttccagcct ggcagcgaag ccgtgaaagc
     1561 cttggctgcc ttaccaccca acgctgatga tgctgctgcg cgcatagctt gcttgcggga
     1621 gatgggtgac ctcaccaccg ctgggttggt ggctcgagat tatccccagc atcccctggt
     1681 gctactgcaa ttagccctca cattattgga cgaaaaaccg cgccaggcga tggctgccat
     1741 ccatgcagct gccgactcac tgtctcaggc taccgcaaac cgtcgattcc gatctgcttt
     1801 tagccttaag gagatctccc cactcgtcta tgccctgcta gccaaccttt tccaccgtga
     1861 tggcaaccgc gtcagcgatc gagataacgc cttacaagcc atactgaatg cactttcaat
     1921 atggccggat gagcctcgct ggcatgtgtt agccgccgag atctatttgg caggtgatcc
     1981 aatggatagc cagggtgatc tggaagcggc gatcaaccac ttggagcgtg ccacaaagct
     2041 cgatccagaa tataccccct cgttaatcgt gctcgggcaa atgtacctaa gggaaggctc
     2101 aatacaaagg gcaatgcaaa ctttcgaaca ggctgcacag gctgctccgg gtgaagccga
     2161 gccatggatg tggcttgctc aagcctgccg tagtatgggc aaaatggaac aagctgctgc
     2221 acacgccgaa cgggctgtca cactggctcc caactatatc aagcccctga ttctacgcgg
     2281 cgagatcgcc ctgcaagatg aaaatccacg cggggcactg agccgggccg aggccgctct
     2341 acgtatccaa ccagatgatc ccgacgcgct cctgctacaa gtgcgcgcct taaatgccct
     2401 ggatcggtat gaggaagccc tcggaacgct tgagaaagct ctttctctgg cttccgatcc
     2461 gctgcccctc tccatggaaa gagtaagact gcttcgacgt gcccagggaa acgaggctgc
     2521 cctccacgcc atccaatctt tggccgatca ctatcccgat gaaccccgcg tgatgacgct
     2581 gcaagcggag gtgctggaag ccgcgggtga cagtgaagct gctattcgcg cagctcaacg
     2641 cgcgctgcgc ggccaagctg gagcaccagt gatctcaaag gtcgatcaag caagtttgca
     2701 cttcatgctc ggcagcctgt tgcgcagcac tgggcagctt gaccagtcgt tgcaccatct
     2761 gagcgagtca atccatttag cacccgatca agtaaacggc tacctggaga tggggcacgt
     2821 ccacctggag cgccgcgagc acacccaagc attaagcgcc ta
//
LOCUS       DBNHOH010000611         2018 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_998891, whole genome shotgun sequence.
ACCESSION   DBNHOH010000611 DBNHOH010000000
VERSION     DBNHOH010000611.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_998891"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="AC16V7_10660"
     CDS             complement(<1..578)
                     /locus_tag="AC16V7_10660"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR6257623.1"
                     /translation="MYSLNDSKTKLISDVLPINKAHVDPNSRPGLQPKNLLIHPIQVS
                     SMQNAVNRYMSSARSLHLLIERNGTEIAQMVDLDRVAVHASDYDQTSLGIGLIYPGYL
                     TEKSGFFYSKNRFDPLEILFAQAINDNKKRWYPFYPQEQLDALLDIARLLSQSYGLER
                     ILVSQEINQFDLISGPAFPINRLRQLVEGEGTA"
     gene            complement(897..1274)
                     /locus_tag="AC16V7_10665"
     CDS             complement(897..1274)
                     /locus_tag="AC16V7_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257624.1"
                     /translation="MSLRNLFIASSIIGFIFGLGFYLAPAQTIGPYGVEPPTQSTLYF
                     IRFVGASFIGFSLLAWLVKDEPASDTRQKILLSYFVSYLLILIVMLIEQLTTPVGALV
                     WVNIVIQALFTAGFGYFRFIKTS"
     gene            complement(1487..1900)
                     /locus_tag="AC16V7_10670"
     CDS             complement(1487..1900)
                     /locus_tag="AC16V7_10670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6257625.1"
                     /translation="MDLIIKAANATAGKILIVDDDPDTCQLLQFLFQRKGYQTKAVTS
                     GWQAIQHVEDQPPDLVVLDVLMLEMDGWETFRRVRELSNSLVLFLSALGGGEHAAQAL
                     QMGADDFIRKPYSNVELLARVEMALSRARGNTSRA"
BASE COUNT          469 a          490 c          589 g          470 t
ORIGIN      
        1 gctgtgccct caccctccac cagctgccga aggcggttga tgggaaaagc cggacccgag
       61 atgagatcga attggttaat ttcttggctg accagtatgc gctcaagccc ataagactgg
      121 ctcaacaggc gggcgatgtc cagcaaggca tctagctgtt cctgggggta gaacggatac
      181 cagcgttttt tattgtcgtt gatggcttgc gcaaaaagga tctcgagtgg gtcaaaccga
      241 tttttagagt agaaaaatcc cgatttttca gtaaggtagc cgggatagat cagcccaatc
      301 cctagcgagg tctgatcgta gtcgctggcg tggactgcca cccggtctaa atcgaccatt
      361 tgggcgatct ctgtgccatt gcgttcgatc agcaggtgca aagacctggc gctcgacata
      421 tatcgattga ccgcgttttg catggatgac acctggatgg ggtggatgag gagattttta
      481 ggctgcaagc cgggcctgct gtttgggtcc acatgcgctt tattgatcgg caggacatcg
      541 gagatgagtt tggtctttga atcgttcaga gaatacatag ggttgctcct aatgtggatg
      601 cgttgagact atgaaggcta acgagaaagg gcctggtgga aaacgcagga agtgccctgc
      661 tcatcacctt tcaggcttgg aggtgggcgc ccgcctcgct cgggcggatc agatcgattt
      721 gcggcgatag cgtgctatgc gggaggtgtc gagctcgagg tgtggatggg gtgcaaatct
      781 gttatggatt gtatagtaat tactggtgtc ggtcaagaaa gggggctggg cgcgcgccca
      841 gaaggaagcg ccgcgcgcgc acctcctgtg gaaaacacca gaactggttg ctgagcctac
      901 gaagttttta taaaacggaa atacccaaag ccagcggtaa acagcgcctg gataacaata
      961 ttcacccaaa ccagcgcgcc caccggagtc gtcaactgct caatcagcat gacgatcaat
     1021 atgaggagat aggagacaaa gtagctcaga aggattttct ggcgggtatc cgaagccggt
     1081 tcatccttga ccagccatgc caacaagcta aatccaataa aagacgcgcc aacaaaacgg
     1141 atgaaataca gggtcgattg ggttggtggt tctacaccat acgggccgat cgtttgggct
     1201 ggagcaagat aaaagcctaa accgaaaatg aagccaatga ttgaagaagc gatgaataga
     1261 ttgcgcaaac tcatgataaa ttcctccttt ttcttaacaa tagatgcttt ataaaagtaa
     1321 cacctgaaag ctgtcagggt tgcgggtttg cgcatgcctc tcagtgcata tgcaattgcc
     1381 ctggcggctg actggcatcc tggcctcgct gtcgctactg gtctggttgt cttctcagga
     1441 tttggggaga ttctgcgctg agatttcttc gtggaaatgg cgcaatctag gcgcggctgg
     1501 tgttgcccct ggcgcgggag agagccatct cgacgcgcgc cagcagctct acgttcgagt
     1561 agggtttgcg gatgaagtcg tcggcgccca tttgtagcgc ctgggcggcg tgctctcccc
     1621 cacccagagc cgagagaaag agaaccaggg aattggatag ctcccgcacg cggcggaagg
     1681 tctcccagcc gtccatctcg agcatgagca catccagcac caccaggtcg ggaggctggt
     1741 cctccacgtg ctgtatggcc tgccagccac tggtgaccgc cttggtttgg taacctttcc
     1801 gctgaaagag aaattgcagc aactggcagg tatcgggatc gtcatccacg atcaggattt
     1861 tacccgctgt cgcgttagcg gccttgatga ttaggtccaa gggattctcc tacactgcgt
     1921 ctttcttaaa taccccattt ttcagtgacg ggatgatcag ggttgggatc cggtgttgtc
     1981 gctatgtccg ggcgcgcgct tacaaatctc ttaggtct
//
LOCUS       DBNHOH010000612         5038 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1767563, whole genome shotgun sequence.
ACCESSION   DBNHOH010000612 DBNHOH010000000
VERSION     DBNHOH010000612.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1767563"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /locus_tag="AC16V7_10675"
     CDS             <1..1157
                     /locus_tag="AC16V7_10675"
                     /inference="COORDINATES: protein motif:HMM:NF024298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HIR6257626.1"
                     /translation="HLTPLGSDALRVVPVLAALGLAACASLPTTPTPTHPPIQTKQPF
                     QVAETSTPFATDTAVASPSVTLSPLPPSLTPTPPAISPANASSLIPRAKIQFSSWELV
                     LAAAWSPDGETLAVAAGEKIHLYRAGSHEERLTLNPGVWTPGLAFSPDSRTLACGGRD
                     GVVQLWDVASGDIRFSMPAHKKGVNSVAFSPDGRMLASAGNDGMVRLWDSENGGPQGQ
                     MIGGAYAIPSVVFTQDSASLAIANGDIIRFRDVETQRFVLTLRGDSSFYSLALAPDGQ
                     FLASGDSNNTVLLWDLSTEKVSHSLQAHNGDVSRAAALIWRVAFSPDARLLVSAGGDQ
                     TVRVWDATTGQLLSTLIGHKAAVTSLAFSPDGRWLVTGGLDATVFVWGSK"
     gene            1290..2183
                     /locus_tag="AC16V7_10680"
     CDS             1290..2183
                     /locus_tag="AC16V7_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR6257627.1"
                     /translation="MRLLCLSLLLILSILLSACVASPTSPSDVEASTYIQNKGSDTIV
                     NLALAWAERYQQEHPNVRISVTGGGTGTGMAALISGTVDIANASRQIKPEEVDEANKN
                     GVEPQEYVIARDAIAVIIHPDNPINKLTLEQVSRIYRGEINNWKELGSYDRPIVRVSR
                     ETNSGTHVYFLESVIRLGNDDNKYFFSTDTLLLPSSEGIIAEVSENPNAIGYDGLGYV
                     TPSVKVVALSEDPAGHYVLPSSETVNNGSYPVSRDLYMYTGSAPSAAAQAYLQWIMEA
                     EAQEIVIELGFVPVNGQAEIR"
     gene            2190..3086
                     /gene="pstC"
                     /locus_tag="AC16V7_10685"
     CDS             2190..3086
                     /gene="pstC"
                     /locus_tag="AC16V7_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HIR6257628.1"
                     /translation="MKSDATKNEFWITLLIRISGYSSILFVILILYFLLREGLPTLGD
                     VSFFSLFGTRWYPIEDYFGVLPLLSGSLIVTVGAMLVALPFGVGTAVFLSEVSPHWLK
                     SVLKPVIEVMAGLPSVVLGFLGIQIAAPLFRRFLDLPTGLTGFTGAILLGFISIPTIV
                     SIAEDALNSVPATYRHGALALGATRWQTIWGVTVPAARSGILTAVMLGVGRAIGETMA
                     VMMVTGNAPVLPFRLDALFLPVRTMTATIASEMGEVAAGSSHYHVLFFIGIILFGVSL
                     IVNIIASSIALRSRSRAERLLS"
     gene            3098..3964
                     /gene="pstA"
                     /locus_tag="AC16V7_10690"
     CDS             3098..3964
                     /gene="pstA"
                     /locus_tag="AC16V7_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR6257629.1"
                     /translation="MDRSDHITNVRRIEWIGINLLRLITLITALPVIGVILYILARGM
                     PAMSLEFLTQMPREGMRAGGILPTIIGTFYLTLGTAIFSVPLGVAAGIYIAEYAPDNY
                     LTRLIRIAIINLAGIPSVVYGLFGLGLFVLFMGFGTSILAASLTLSIMTLPVIISTTE
                     EALRSVPNSFRVVSLSMGGTKWQTVWRITLPQALPGVITGIILGLERAAGETAPILFT
                     GAAFFLPRLPHSPLDATMALPYHIYVISTSVPGMPVQIQYGTVLVLLVFVLGMNLIAT
                     LIRNRARARRQW"
     gene            3972..4730
                     /locus_tag="AC16V7_10695"
     CDS             3972..4730
                     /locus_tag="AC16V7_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HIR6257630.1"
                     /translation="MEKLIIVEKLNLVYSDGVESLVDVNLDIIANSVNVLFGPAGGGK
                     SSLLRAFNRLNDLADVASISGRILFHGMDILDPKTDVIDLRRKIGMVFSRPVPLPLTV
                     YENVSYGLQLAGERSKSRLDEAVEEALRLAVLWDEVYDRLNDPAYSLSGGQQQRLCLA
                     RVLALKPDVVMLDEPTSALDPVSTARIEALLLDLRERFTIILVPHNVQQAARTADYAG
                     FFLQGKLVDYAPGKELFLNPKLKETQDYVTGRFG"
BASE COUNT         1087 a         1410 c         1303 g         1238 t
ORIGIN      
        1 ggcaccttac gcctttgggg agtgacgcct tgagggttgt gccggtgctg gccgcattag
       61 gccttgcagc ttgcgccagc ctgccgacaa cacccacccc cactcatcca ccaatccaaa
      121 ccaagcagcc tttccaagtg gccgagacat ctacgccatt tgccaccgat actgcagtgg
      181 cttccccatc cgttaccctc tcccctctac ccccatctct aacgcccact ccgcctgcga
      241 tctctcctgc taacgcctct tccctcatac cacgggcgaa aatacagttt agctcttggg
      301 aactggtact tgcggcggcc tggtctccag atggcgagac gctggcggta gccgctgggg
      361 aaaagattca cctttatcga gcgggatcac acgaggagcg gctcaccctg aatccgggtg
      421 tatggacgcc tggactggca ttcagcccag atagccgcac gctggcttgt ggtggacggg
      481 atggcgtagt acaactgtgg gatgtcgcat caggagatat ccgcttctct atgccagctc
      541 acaagaaagg ggtcaactcc gtcgccttta gcccagacgg caggatgctg gcatcagccg
      601 gcaatgatgg catggtacgc ctatgggatt ctgagaatgg cggtcctcaa gggcagatga
      661 tcggtggggc gtacgctatt ccatccgtgg ttttcaccca agacagcgcc agcctggcaa
      721 tcgccaacgg ggatattatc cgtttccggg atgtggaaac gcagcgcttc gtccttacgt
      781 tgcgcggtga ttcctccttc tattccctgg ctctcgcgcc tgatgggcag ttcttagcct
      841 caggcgatag caacaacacc gtgctgctat gggatctttc gaccgagaaa gtcagccaca
      901 gcttgcaggc tcataatggg gatgtttcac gggctgctgc gctgatctgg cgagtcgctt
      961 ttagccccga cgctcgcctg ctagtctctg ccggagggga tcagacggtc agggtgtggg
     1021 acgctaccac gggtcagctg ctgtcaaccc tgattggtca taaggctgca gtcaccagcc
     1081 tggcatttag cccagacggc cgctggctgg ttaccggcgg gctagatgcg accgtattcg
     1141 tctggggatc gaaataggga aaatatttct ctctcacaac agctccaaca tatgccttca
     1201 tcaatatgcg tagaatcccg tctcaacctg cagagatgtc agcgatcttt aaggtttatc
     1261 ccaaagagat tagactataa tttgttgaaa tgaggctttt gtgtctctct ctacttttaa
     1321 tcttgagcat cctgcttagt gcgtgtgtag caagccctac atccccttcg gacgtagaag
     1381 cttctaccta catacaaaat aaaggatccg acactatcgt caacctggct ctggcttggg
     1441 ctgagcgcta ccagcaggag catcccaatg tacgtatctc ggtaaccggt ggtgggacag
     1501 gcactggtat ggcagcgctg atcagcggca ctgtggacat agccaacgcc tctcgccaga
     1561 ttaaacccga agaggtcgat gaagctaata aaaatggggt ggagccgcag gaatacgtga
     1621 ttgctcgcga tgcaatcgca gtcattattc acccagataa ccccataaat aaacttaccc
     1681 tcgagcaggt ctcaagaata tatcgcgggg aaatcaataa ctggaaagag cttggctcgt
     1741 atgaccgccc gattgtgcgc gtttcgcggg agaccaactc aggtacccac gtttactttt
     1801 tagagtcggt gatccgcctg ggtaatgatg ataacaaata tttcttctcc accgacacgc
     1861 tcctgctgcc atcatcggaa ggcatcatcg cagaggtcag tgaaaaccca aacgccatcg
     1921 gatatgatgg gctaggctat gtaaccccca gtgtaaaagt ggtcgctcta tcagaagacc
     1981 cggctggtca ctacgtgcta ccatcgtctg aaacagtcaa caacggaagt tacccggtat
     2041 cacgtgactt gtacatgtac acggggagcg ccccttccgc ggcagcgcag gcttacctcc
     2101 aatggatcat ggaggcggag gcgcaggaaa ttgtcattga attaggattt gtacccgtaa
     2161 atggccaagc agaaatcagg taaacatcaa tgaaatcaga tgcaacaaag aacgaattct
     2221 ggatcacact gcttatacgc atctcaggat actcaagcat attatttgtc atcctgattc
     2281 tatatttcct tcttcgcgag gggctgccaa cgctgggaga tgtctcattt ttcagcctgt
     2341 tcggcactcg ttggtatccg attgaggatt actttggagt gcttcctctc ctcagcggtt
     2401 cactcatcgt caccgttggc gcgatgcttg tcgccttgcc gtttggcgtg ggcactgctg
     2461 ttttcctttc ggaggtatct ccccactggc tcaagtcagt gttgaaaccg gtcatcgaag
     2521 tcatggccgg tctaccttcg gtggtgctgg gctttctagg aatccagatc gcggctcccc
     2581 tcttccgccg tttcctggat ttacccaccg gattgaccgg attcacgggt gcgatcctgc
     2641 ttggctttat ttccatcccg acaatcgtct ccatcgccga agatgcgctg aattcagtgc
     2701 ccgctacata ccggcatggg gcgctggcac tgggtgctac ccgctggcag acgatctggg
     2761 gagttacggt gcccgcggcg cgctcaggca ttctcaccgc agtgatgctg ggagtcggcc
     2821 gggcaatcgg cgagaccatg gcggtgatga tggttaccgg aaacgccccg gtgctgccat
     2881 ttcgcctgga cgcattgttc cttccagtgc gcaccatgac cgccaccatc gcttcggaaa
     2941 tgggagaagt agctgcaggc tcgtcccact atcatgtact cttttttatc ggcattattc
     3001 tgtttggcgt ctcgctaatc gtaaacatca tcgcatcttc gattgcatta cgcagccgct
     3061 cgcgagccga aaggcttctt tcttaggtag agagctgatg gatcgatctg accacattac
     3121 gaatgttcgc cgcatcgaat ggattggcat taacctgctg cgcttgatca ccctgatcac
     3181 cgctctgcca gtcatcggtg ttatcctata tatccttgca cgcgggatgc ccgcaatgag
     3241 ccttgagttt ctcacccaga tgccgcgtga aggcatgcgc gcgggtggga tattgccgac
     3301 tattatcggc actttctacc tgacccttgg cacagccata ttttctgtac ccttgggtgt
     3361 ggccgccggc atctatatcg ccgaatacgc accagataac tacctaaccc gtctcatccg
     3421 catcgcaatc atcaatctgg ctgggatacc ttctgtagtc tacggtctct ttggcctggg
     3481 cttgtttgtc cttttcatgg gtttcggcac cagtattctg gctgcctcgc tcaccctctc
     3541 gattatgacc ttgccggtga ttatcagcac caccgaagag gcgcttcggt ctgtccccaa
     3601 ctcgtttcgc gtggttagct tgtctatggg ggggaccaag tggcaaaccg tttggcgtat
     3661 caccctgcct caggcacttc caggcgttat taccggtatc atcctcggcc tagagcgtgc
     3721 agcgggggag acagcaccga tcctcttcac gggcgccgcc ttcttcctgc cccgcctgcc
     3781 acattcacca ctggacgcca ccatggcact cccttaccac atttacgtga tttcgacctc
     3841 agtgccgggt atgccggttc agatccaata cggaactgtg ctggtgctgc tggtatttgt
     3901 actgggcatg aacctgatcg caacacttat ccgcaaccgt gccagggcgc ggcgccaatg
     3961 gtaggtccaa catggaaaaa ctgatcattg tcgagaagtt gaacctggtc tattcagatg
     4021 gcgtcgagag cctggtcgat gtaaacctgg acatcatcgc taattcggtg aatgtgcttt
     4081 ttggccctgc aggtgggggc aaatcttccc tgctgcgcgc cttcaaccgg ctgaatgatc
     4141 tggcagatgt agccagcatt tccggacgta tccttttcca tgggatggac atcctcgacc
     4201 cgaaaactga tgtgatcgat ttgcgccgca agatcggcat ggtcttttca cgacccgtac
     4261 cgttgccgtt gacagtttac gagaatgtgt cctatgggct gcaactagct ggagaacgta
     4321 gtaagagccg tctggacgaa gcagtcgagg aagcgctgcg cctggcagta ctgtgggacg
     4381 aggtgtacga caggctcaac gacccggctt actcgctttc cgggggacag caacagcgac
     4441 tttgcctggc gcgagtgctg gcgttaaaac ctgatgtagt tatgctcgat gagcccacct
     4501 cagcactgga tcccgtatcc accgcacgca tcgaggctct actgctagac ctgcgtgaac
     4561 ggttcaccat tatcctggtg ccgcacaatg tgcagcaggc agccaggacc gcagattacg
     4621 ccggcttctt cctgcagggc aagctggtag attatgcccc tggcaaagaa ttattcctga
     4681 atcccaagct caaagagacc caagattacg tcactggaag atttggttaa atcgtagatc
     4741 gctggtgttg aaacgtcagc gatgcagatc atatactgca caacgacatc caggggggtg
     4801 aattgaattt tatctagcca aattgattga atatccccat tcaattacgc ggttaaattc
     4861 ctttggatat gctctgaccc cttattatcc gcttggtctt caagtaagct tccacgaagc
     4921 aggttttatt ctcgagcttg gttaaaccga acaaagctga atctgccgat tgtctaaatc
     4981 cagtttctca tccttcgcga tgaagatcct caaatcccaa gaaccaggta gcgaatta
//
LOCUS       DBNHOH010000613         7082 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1125822, whole genome shotgun sequence.
ACCESSION   DBNHOH010000613 DBNHOH010000000
VERSION     DBNHOH010000613.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7082)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7082)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7082
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1125822"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="AC16V7_10700"
     CDS             complement(<1..771)
                     /locus_tag="AC16V7_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR6257631.1"
                     /translation="MEISLNVNDAVHTLDVPAGYTLLKVLRHLGYFGTKHGCETGECG
                     ACTALLDGKPVNSCLVLAAQAQGHRIQTIENIGQHPEQGWKLTEGLHPLQQAFVESGA
                     IQCGFCTPAQILAAKELLDRNPTPSEAEVREAFSGVLCRCTGYLKPVQAVLRAAAAMR
                     GEQVENGELVGFIFPESRDGEPFGEATSSPTPYPLSPATSVQALPKVFVAPETETWQR
                     VGKPEIKVDAVKLVQGKPAFAADMDARGLLYAKVLHSPH"
     gene            complement(812..1576)
                     /locus_tag="AC16V7_10705"
     CDS             complement(812..1576)
                     /locus_tag="AC16V7_10705"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR6257632.1"
                     /translation="MVIEYNRPKTLDEALSLLSRKGIKTLPLGGGTILNQPSTERYAV
                     VDLQSLGLDTIQARGNTLTLGATVTLQKALESGRLPDRLDKVIRHEASYNLRQVATLA
                     GTLVSADGRSPFATALLALDAALEIKSASDPSERISLGDLLPVRGERLAGRLITSVTL
                     PLNIQLVYEYVSRTPEDLPIVCVALARWPSGRTRLVLGGFGEAPLLALDGPEAGGAEV
                     AARDAYSRAGDEWASAEYRQDVAAILTRRCLSTITG"
     gene            complement(1643..2116)
                     /locus_tag="AC16V7_10710"
     CDS             complement(1643..2116)
                     /locus_tag="AC16V7_10710"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR6257633.1"
                     /translation="MRQPNSRHCFVCGLANPHGLQLRFEITAPGEVVANYTVPERYEG
                     YPGVVHGGIVAAMLDEVVSRVHMGIDPPRFMYTARLELRYRKNVPIGQPLRIVGRAEK
                     SKGRTATSKGAIYDQNGDILAEAEALLVNVPDEMLSDVDLRALGWRIYDDEEIRG"
     gene            complement(2113..3108)
                     /locus_tag="AC16V7_10715"
     CDS             complement(2113..3108)
                     /locus_tag="AC16V7_10715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6257634.1"
                     /translation="MKRVALYLQDAHDLRDGLDYVRYAEQRGFEAVWQAESRLVRDAI
                     VPMAAYASVTERIKVGSGVINNWTRNIGLLAATFLTLDDLAPNRIICGIGAWWDPLAR
                     NVGIERRKPLLAMRETVEVLRRLLKMERVTFHGEFHQVDGIELDVVHGRREPRHVPIM
                     IGATGDKMMEMTGEIADGVVLNYCVPPEYNDNAMELLEAGAKRAGRKLDDIDRPQLVV
                     CSVDHDRERAIDTTRELLTQYLAQQPHIAKASGVSEDVVKEIQAILGWPATKEQIRRA
                     KHLVPEELIHRITASGTPDEARAKVQEYINHGATCPILYPVGGDVRLLVDTFAVV"
     gene            complement(3124..3726)
                     /gene="cofC"
                     /locus_tag="AC16V7_10720"
     CDS             complement(3124..3726)
                     /gene="cofC"
                     /locus_tag="AC16V7_10720"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HIR6257635.1"
                     /translation="MTLWAIVPVKPLRRGKSRLAEVLTQEERKDLNCNLLAHTVDTLA
                     AIPDIEHVLVVSRDPEALALAREHGARTVQENGASGLNIALAKATIVAKNYTTRGVLI
                     VPADLPLITPEDVRAILERVKDPPVVVVAPDQRRQGTNALLICPVGLIDYNFGPKSFE
                     RHCELAVQAGARLEICELPSLALDVDLPEDLDAVGREFES"
     gene            complement(3794..4804)
                     /gene="cofD"
                     /locus_tag="AC16V7_10725"
     CDS             complement(3794..4804)
                     /gene="cofD"
                     /locus_tag="AC16V7_10725"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HIR6257636.1"
                     /translation="MKAEPLASLKVVALAGGVGGAKLADGLAQILPSNNLAVIVNTGD
                     DFDHLGLRICPDLDTVCYTLAGIANPATGWGRSGETWNAMESLAILGGPQWFRLGDLD
                     LGTHLERTRRLQLGDRLSQITRDFCRAWGVQLTVLPMSDDAVATWVYTDAGVLPFQEY
                     FVRQRCQPQVRGFRFDGVERALPAPGVLDVLESADVVVICPSNPWVSIEPILSVPGIR
                     EAVAAKFTVAVTPIIAGRAVKGPAAKMCTEMGIEPSAVSVAQHYSSLLDAFVMDQVDV
                     VQFEALNAQGMPTLATNTLMTSADDRRRLAKEVIEFGMHMLEKKPPKRHNRNIGFTQV
                     KE"
     gene            complement(4859..5470)
                     /locus_tag="AC16V7_10730"
     CDS             complement(4859..5470)
                     /locus_tag="AC16V7_10730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR6257637.1"
                     /translation="MSSNQDYQDFIRSRRSVRRFLPDRVPQELLVRVLQTAAWAPSAH
                     NRQPWRFVELDSALARERLALAMGQDYQRDLHADGASEEQIQARLQRSRQRITEAPAA
                     ILLCLETDEIDRYPDEHRQHAEYLMAVQSVAMAGQNLLLAAHAEGLGGVWMCAPLFAQ
                     EAARGALNLPESWQPQALVLLGYPAKVSPPRIRREMDEVVLHV"
     gene            complement(5467..>7082)
                     /locus_tag="AC16V7_10735"
     CDS             complement(5467..>7082)
                     /locus_tag="AC16V7_10735"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012340.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HIR6257638.1"
                     /translation="GVCAETVWKYKVGQADQVLTHRRADDAGKRPLGAYFRVNHKDLV
                     AMHAAITEVGILYATGNVHDGWGRINSKGVIFYDQNVKIRGGHAFAIVAYDEEGFWIQ
                     NSWSATWGQGGFAKITYDDWMANAMDVWVARLGVPVKRLTDQGTAMNRSPGAHRSEAY
                     SYVDVRPHVISLGNDGKLSDQGTYGTSKEEVKRIFQDDIPRVTKNWDKIRILLYAHGG
                     LVGESSFLQRVAEYRAALLNVKVYPLAFVWHTDLWSTVTNILKDAVSKRRPEGPLDNA
                     MDFMLDRLDDTLELIVRGLKVKGLWDEMKENAWRATLLSDGGARLTAHYLSDLMDKNG
                     NVEVHVVGHSAGSIFHAPLIQYLTTKGEIPGNQWSNKTQGLGRKITSCTLWAPGIRMN
                     EFRQTYLPAVQSGAIERFALYTLTDEAERGDHCAGIYHKSLLYMVSNALEENFSEPIL
                     GMEKYVKEDTDLKALFSDGVARWVKAPDHTGEPPVSKSSKHGDFDDDTPTLMSTLAFM
                     VPSMKRPETDVFKFRRSSASLSNRRRQLG"
BASE COUNT         1598 a         2135 c         1785 g         1564 t
ORIGIN      
        1 atgggggctg tgcaagacct tggcgtaaag cagtccacgg gcatccatgt cggcggcgaa
       61 ggcgggcttc ccctgcacca gcttaaccgc gtcaaccttt atctcaggtt tgccgacccg
      121 ctgccaagtt tcggtttctg gggcaacaaa taccttcggc agcgcctgaa cggaagtggc
      181 tggggaaagt ggataaggcg ttggagatga ggtggcctca ccaaacggct ccccatctct
      241 tgactcggga aagataaagc cgaccaactc accattttct acctgctctc cacgcatcgc
      301 cgccgcggca cgcagcaccg cctggacagg cttgagatac cctgtgcaac ggcacaatac
      361 accagaaaag gcctcgcgta cttctgcctc gctaggggta ggattgcgat cgagcaactc
      421 cttggcagct aaaatttggg ccggggtgca gaaaccgcat tggatcgccc cgctctccac
      481 aaatgcttgc tgaagcggat gcagaccttc ggtgagcttc catccctgtt ctggatgctg
      541 gccaatgttt tcgatggtct gaatgcgatg accttgagcc tgagcagcca ggaccaagca
      601 agagttgacc ggtttgccat ccagcaacgc agtacaagct ccgcactcgc cagtttcgca
      661 gccatgcttg gtgccaaaat agcccaaatg tcgcagcacc ttgaggagtg tatatcccgc
      721 tgggacatcc aatgtatgca ctgcgtcatt gacattaaga ctgatttcca tacgatctcc
      781 aaccatcaat tctgcttaaa actcaccacc ttcaaccggt aattgtcgac aaacatcgtc
      841 tggtgagtat tgcggctaca tcttggcggt attcggcgct ggcccattca tcaccagcgc
      901 gactatacgc gtctcgcgca gccacctccg caccacccgc ttctggtcca tccagagcca
      961 gaaggggagc ttctccaaaa ccgcccagaa ccagacgcgt gcggccagac ggccagcgtg
     1021 ccaatgccac gcaaacgatc ggtaggtctt caggcgtgcg cgacacatac tcgtaaacca
     1081 attgtatgtt gaggggcaat gttacgctgg tgatcaagcg tccagccagg cgctccccgc
     1141 gcaccggaag gagatccccc agacttatcc gctcggaagg atcagaggcg cttttgatct
     1201 cgagggcggc atctagggct aacagcgcag tggcaaaggg cgaacgccca tctgcggaca
     1261 ccaatgtccc cgccagtgtc gcaacctggc gcaagttata gctggcttca tggcggataa
     1321 ctttatccag cctatcgggc aatcttccgc tttcgagcgc cttctgcaat gtcaccgtcg
     1381 ctcctagcgt caaggtattg ccgcgggcct gtatggtgtc taaacccaga gactgaagat
     1441 cgaccacagc gtatcgttcc gttgaaggct ggttcaggat tgtcccccca cctaggggca
     1501 gtgttttgat acccttacgg gaaagtaacg aaagcgcttc atccagcgtt tttggacggt
     1561 tatattcaat caccataaac tgactcccca attcccagca gtaaagaaga tgcgaaatca
     1621 gtttgcacgg ataatctctc tcctaccccc ttatctcctc gtcatcatat atacgccaac
     1681 ccaatgcccg caaatccaca tcgctcaaca tttcatcagg cacattgaca agcaaggctt
     1741 cggcttccgc caggatatca ccgttttgat cgtagatggc accctttgac gtggcagtgc
     1801 ggcctttgct tttctccgcc ctgccaacaa tgcgtaaggg ctgaccgatc ggcacattct
     1861 tgcggtagcg caattccaag cgggcagtgt acataaagcg cggcgggtcg atacccatat
     1921 gaacacggct gacaacttcg tctagcatgg ctgccacgat accaccgtgc accacgccag
     1981 gatagccctc gtagcgttct gggaccgtat agtttgctac aacctcacca ggtgcagtaa
     2041 tttcaaagcg caactgcaag ccatgcggat ttgctaggcc acagacaaaa cagtgacgcg
     2101 agttgggttg tctcatacta ctgcaaaggt atcaactaat agcctcacat caccacccac
     2161 cgggtacaag atggggcaag tggcgccatg gtttatgtac tcctgtacct tagctcgagc
     2221 ttcatcaggt gtgcctgaag cggtgatgcg gtgtataagt tcttcaggca ccaggtgctt
     2281 ggcgcggcga atttgctctt tggtggctgg ccaacccaga atagcctgga tctcctttac
     2341 cacgtcttcc gagacacccg aggccttggc gatgtggggc tgctgcgcca ggtattgggt
     2401 gagcagctca cgcgtggtat caatagccct ctcgcggtca tggtccaccg agcagaccac
     2461 cagttgcggg cgatcgatat catccaactt gcgaccggca cgcttggcgc ccgcctctag
     2521 taattccatg gcgttgtcgt tatattctgg gggcacgcag tagtttaaca ccacgccgtc
     2581 cgcgatttca ccggtcatct ccatcatttt atcgccagtg gcgccaatca taatgggcac
     2641 gtgtcgtggt tcccgccgtc cgtgaaccac gtctagctcg ataccgtcca cctgatgaaa
     2701 ttcgccgtga aaggtcaccc gctccatttt cagcaggcgg cgcagcactt ccaccgtctc
     2761 gcgcatcgcc agcagtggtt tgcgccgctc gatacccaca ttgcgtgcca ggggatccca
     2821 ccaagcacca atcccgcaaa tgatgcggtt tggtgccagg tcgtctaggg ttaagaaagt
     2881 ggctgccagc aatccgatgt tgcgggtcca attgttaatt acgcctgagc ccaccttgat
     2941 acgctctgtg accgacgcat aagctgccat cggcacaatc gcatctcgca ccaagcgcga
     3001 ttctgcctgc cacacagcct caaacccgcg ctgctcggca tagcgcacat aatccagccc
     3061 atcacgcaaa tcatgggcat cttgcagata aagggcaact cgcttcattc cataaactcc
     3121 ttactaggat tcgaactctc tccctaccgc atccaagtct tctggcaaat ctacatcaag
     3181 agccagagat ggcaattcgc aaatttccaa ccttgctcca gcctgcaccg cgagctcgca
     3241 gtgtcgctca aatgactttg gtccaaaatt gtaatcgatt aatcctaccg ggcagatcag
     3301 cagagcattc gtgccttgcc ggcgttgatc tggcgctaca accaccactg ggggatcttt
     3361 gacacgctct aaaatagcgc gaacatcttc tggggtaata agaggcagat cagctggtac
     3421 gatcagcacg ccacgcgtgg tgtaattctt agccacaatt gtagccttag ctagggctat
     3481 gttcaaaccc gatgccccat tctcttgcac ggttcgcgcg ccatgctctc gagccagggc
     3541 aagcgcctca ggatctcggc tgacgactag cacatgctca atatctggga tagccgccaa
     3601 cgtatctacc gtatgcgcca gcaaattgca attaaggtct ttacgctcct cctgagtgag
     3661 tacttccgcc aagcgcgact tacctcgtcg cagaggtttg actggtacga tggcccatag
     3721 tgtcatgcat cagtgatcct ttctgaatca tatagaattt acgcagcagg aaggcagaga
     3781 agtgacttta gctttattcc ttcacttgtg taaatcctat atttctatta tggcgcttgg
     3841 gcggcttctt ctccaacatg tgcattccaa attctatcac ctctttggcc agacgtcggc
     3901 gatcgtcggc tgaggtcatc aatgtatttg tcgccaaggt tggcattccc tgggcgttta
     3961 gcgcttcaaa ttgaactacg tccacctggt ccatcacgaa agcatccaac aacgagctat
     4021 aatgctgtgc taccgagacg gcagaaggct cgatccccat ctctgtacac attttagcag
     4081 cgggaccttt gacggcgcgc ccggcgatga tcggcgtcac cgccacagta aactttgccg
     4141 ccacagcttc ccggataccc ggcaccgaaa gaatcggctc tatgctcacc cacgggttgg
     4201 atgggcagat aaccactaca tcagcggatt ccaagacatc caaaaccccg ggagcgggca
     4261 gtgcgcgctc aacaccatcg aagcgaaaac ctcgcacctg cggctggcag cgttgacgga
     4321 cgaagtattc ctgaaagggt aacacgcctg catcagtata cacccaggtc gccacagcgt
     4381 catcgctcat tggcaaaact gttagctgca ccccccaggc acggcaaaaa tcacgcgtaa
     4441 tctggctgag tcggtcgcca agctgcaggc ggcgggtgcg ctccaagtgt gtccccaggt
     4501 ctaaatctcc caggcggaac cattgtggac cacccagtat tgccaaactc tccatggcgt
     4561 tccaggtctc gccagaccgc ccccagccgg tggctggatt ggcaatccct gccagggtat
     4621 agcataccgt atcaaggtca gggcaaatcc tcagacccag gtgatcaaaa tcatcaccgg
     4681 tgttgacgat cacggccagg ttgttcgatg gcagaatttg cgcaagccca tccgccaact
     4741 tggctccacc cactccgcca gccagagcca cgactttgag cgaggcaagc ggttcagctt
     4801 tcaccagctc accagaccgc caatacgggc tcggttgatc ttttcaaacc aggatcgatc
     4861 aaacgtgcaa gacaacctca tccatctccc ttcgaatgcg aggcggtgag actttggccg
     4921 gataacctaa caaaactagg gcttgcggct gccagctctc gggaaggtta agggcgcccc
     4981 gcgccgcctc ctgtgcaaac aacggggcgc acatccaaac ccctcccaaa ccctccgcgt
     5041 gagcagctaa taacaagttt tgccccgcca ttgccacgct ctgtaccgcc atcagatatt
     5101 ctgcgtgctg gcggtgctcg tctgggtagc gatcgatttc atcagtctcc aggcagagca
     5161 agattgccgc cggtgcctca gtgatgcgct gacgtgagcg ttgcagtcgt gcttggatct
     5221 gctcttcaga tgcgccatcc gcgtgcaaat cgcgttgata gtcctgcccc atggctaggg
     5281 caaggcgctc ccgagctaat gcgctatcca gctcaacgaa tcgccagggc tggcgattgt
     5341 gggccgaggg tgcccaggca gctgtttgca aaacgcgcac cagcaattcc tggggcaccc
     5401 tgtctggcaa aaaacggcgc acagaacggc gcgagcggat aaaatcctga tagtcctggt
     5461 ttgaactcac ccgagctgcc gcctccggtt gctcagcgag gcactcgagc gtctaaactt
     5521 aaagacatcc gtctcgggtc tcttcatcga cgggaccata aaggccaatg tggacatcaa
     5581 tgtcggtgta tcgtcatcaa agtcgccatg tttgctggac ttggatacag ggggctcacc
     5641 agtatggtcg ggggctttga cccagcgagc gactccgtcg gaaaacagag ctttcagatc
     5701 agtatcttcc ttaacatact tctccatgcc caatatgggt tctgaaaaat tttcttcaag
     5761 tgcgttcgag accatataaa gcagggactt atggtagatg cctgcacagt ggtctccacg
     5821 ctcggcctcg tccgtcaacg tgtagagagc aaagcgctca attgcgccag actgtaccgc
     5881 aggcaggtat gtctggcgga attcgttcat gcggatgccc ggcgcccaca gcgtgcagga
     5941 agtgatcttc ctgcccaagc cctgtgtctt gttggaccac tgattcccag gtatctcccc
     6001 tttcgtggtg aggtactgta tcagcggtgc gtgaaatatg cttccagcac tgtgaccgac
     6061 gacgtgcact tcaacattcc cattcttatc catcaaatcg gacaggtagt gcgcggtaag
     6121 acgcgccccc ccatccgaaa gcaatgtggc tcgccaggca ttttccttca tctcatccca
     6181 caagccttta accttcagac cacgcacgat caattccagc gtgtcgtcca ggcgatccag
     6241 catgaagtcc atggcgttat ccagagggcc ttctggcctg cgtttcgaga cggcatcctt
     6301 gagaatattc gtcaccgtgc tccacaagtc cgtatgccag acaaacgcca gtggatacac
     6361 ttttacattc aataacgcgg cgcggtattc tgctacccgc tgaaggaagc tactctcccc
     6421 caccagaccg ccgtgggcat ataacaaaat gcgaatctta tcccagtttt tcgtgacgcg
     6481 ggggatatcg tcctggaaga tgcgcttgac ctcctctttt gaagtcccat aagtcccctg
     6541 gtcacttagc ttgccatcgt tgcccaggct gatgacatgc gggcgaacat cgacataaga
     6601 gtacgcctca gatctatggg cgccgggaga acggttcatc gccgtccctt gatcagtcag
     6661 gcgctttacc ggcacgccca agcgcgccac ccacacatcc atggcattgg ccatccagtc
     6721 atcgtaggtg atcttggcga agccaccctg accccaagta gctgaccagg aattttggat
     6781 ccaaaatccc tcctcgtcat aggccacgat ggcaaaggca tggccaccgc ggatcttgac
     6841 attttgatcg tagaagatca cccctttaga gttgatccgt ccccagccat cgtgaacatt
     6901 acccgtggca taaaggatgc ccacctccgt gatggcggca tgcatggcga ccaggtcttt
     6961 gtgattcacg cggaaatagg ctcccagggg acgtttgccg gcatcatcag cccgccggtg
     7021 ggtcagcacc tggtccgcct ggcccacctt gtatttccaa acagtctccg cgcagacgcc
     7081 gt
//
LOCUS       DBNHOH010000614         1695 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_164086, whole genome shotgun sequence.
ACCESSION   DBNHOH010000614 DBNHOH010000000
VERSION     DBNHOH010000614.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_164086"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /locus_tag="AC16V7_10740"
     CDS             <1..1247
                     /locus_tag="AC16V7_10740"
                     /inference="COORDINATES: protein motif:HMM:NF016115.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0006569 - L-tryptophan catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR6257639.1"
                     /translation="PEAQGSVESKEFGFLIRHPGPSPDFFKWALDMELKLIGVDCGCA
                     EHPMNTNIRYMHANEFKKAEAKLVADYGKTWDEMFPQDEYYQMTHITLPKAHLLFVES
                     IVGQIEELKNQRAWIMVHPIPFMEVETAWARTCALRPPEGMSEDEFFQVMQSAEMLDL
                     TISFSVQTPQWANYVPLTVTYTKRVGGQYFGMGRNGSICNSSIHLATHMDGEKHFWPA
                     GRTIGEVPLEHWVGPGVIADISGLVSDSSVYTPKMIESVVEVREGDILILKTGWHKYG
                     WNSPDSDEFRYMIKHPGPSPDFSDWVVKKKIKWLGIDAVSQDHPMNTIQRIWHPKTFE
                     EANRKLKRDFGKDWDEMFPLDKFYQDTHLNLFPKKIVHAENLGRDISTAPSGRYYIGC
                     YLQKAMQVESMWGRFVAWKEGR"
     gene            1294..>1695
                     /locus_tag="AC16V7_10745"
     CDS             1294..>1695
                     /locus_tag="AC16V7_10745"
                     /inference="COORDINATES: protein motif:HMM:NF017206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HIR6257640.1"
                     /translation="MMILITGAAGKTGKAIVRKVAEKRGVVKAWVFRAEQIQEVEALG
                     AKEVIVGDMRDSKILRQALQNVDIIYLICPNVHPDETAIASEIIEAAARTGVRQFVYH
                     SVLHPQIEAMPHHWNKMRVEEHIFLSGLPFTI"
BASE COUNT          460 a          411 c          453 g          371 t
ORIGIN      
        1 atcctgaggc tcaaggaagt gtggagtcca aggagtttgg cttcctcatc aggcacccgg
       61 gaccctcacc tgatttcttc aagtgggcgc tggatatgga gttgaagctc atcggcgtag
      121 attgcggctg cgctgagcat ccgatgaaca ccaacattcg atacatgcac gccaatgagt
      181 tcaagaaggc agaggccaag ctggtagcag actacggtaa aacctgggat gagatgttcc
      241 cacaagatga gtactaccag atgactcata tcacattgcc taaagctcac ctgctatttg
      301 tagaatccat cgtaggtcaa attgaggagt tgaaaaacca acgcgcatgg atcatggttc
      361 accccatccc attcatggaa gtcgaaactg cctgggcacg cacctgcgca ttgcgcccac
      421 cggagggcat gagcgaagac gaattcttcc aggtcatgca atccgcggaa atgctcgatc
      481 tcacgatctc gttcagcgtg cagacacccc agtgggcaaa ctatgtgcca ctgacggtaa
      541 cctataccaa gcgagttggc ggccagtatt tcggtatggg caggaatggc tcgatatgca
      601 actccagcat ccacctggca actcacatgg acggtgaaaa gcacttctgg ccagccggac
      661 ggacgattgg cgaagttccg ttggagcatt gggttggacc aggcgtgatc gcagatatct
      721 cggggttggt cagcgactcc agcgtgtata cgcccaagat gatcgagagc gtggtggagg
      781 tgcgcgaagg agatatcctt attctgaaga ctggctggca caaatacggg tggaacagcc
      841 cagattcgga tgagttccgc tatatgatca aacaccccgg gccttcacca gacttctcag
      901 actgggttgt taaaaagaag atcaaatggc tgggcatcga tgctgtcagc caggatcacc
      961 ctatgaacac catccaacgc atctggcacc ccaaaacatt cgaggaagcc aatcgaaagt
     1021 taaagcgcga tttcggtaaa gattgggacg agatgttccc tctggataaa ttttatcagg
     1081 acacccatct caatctcttc ccgaagaaga ttgtccatgc cgagaacctg ggaagggaca
     1141 ttagcacagc gccaagtggt cgatactaca tcggatgcta cctccaaaaa gccatgcaag
     1201 tcgagtcaat gtggggtcgc tttgttgcct ggaaagaagg aaggtaatca ggtaagacct
     1261 ggcaggtttt tttacctgcc aggtcttatt cttatgatga tattaatcac gggtgcagcc
     1321 ggtaaaaccg gcaaggcgat agtaaggaaa gttgccgaga aaagaggcgt cgtcaaggcg
     1381 tgggtttttc gcgccgagca aattcaggaa gttgaagcgc tgggggcaaa agaagtgatc
     1441 gttggagaca tgcgcgattc gaaaatcctg cgtcaagccc tgcagaatgt ggatataatc
     1501 tatcttatct gcccgaatgt ccatccagat gagactgcca tcgccagtga aatcattgag
     1561 gctgcggcaa gaaccggagt aaggcaattt gtgtaccact cagtgttgca cccacaaatc
     1621 gaagccatgc ctcatcactg gaataagatg cgagttgaag aacatatatt cctttcgggt
     1681 ctgccattta ccatc
//
LOCUS       DBNHOH010000615         2464 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1767698, whole genome shotgun sequence.
ACCESSION   DBNHOH010000615 DBNHOH010000000
VERSION     DBNHOH010000615.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1767698"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="AC16V7_10750"
     CDS             <1..326
                     /locus_tag="AC16V7_10750"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6257641.1"
                     /translation="HLLDNACKFTPRGGRVKLEVDQSDNFVKIAVTDTGIGIPAHRMP
                     EIFEPFHQLDGSSTRQFSGTGLGLALSQRILTAHGTQIEVQSQEEKGSRFEFLLPLAE
                     VAMQM"
     gene            335..1585
                     /locus_tag="AC16V7_10755"
     CDS             335..1585
                     /locus_tag="AC16V7_10755"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6257642.1"
                     /translation="MVDETSNPTALRPEELEAVYSISRAVAGAVDLDTALDEIIRLTR
                     SVFIFDNMVLYNLRSIDTLEPAYARAIGRGRSQEAELVWGEATALEAIQKSQVIILQE
                     ESGDQEDRTGVRFSLGLPLRSGKDIKGALVFIRFGGPTYKPDQIRLAEFIAMHVAQLL
                     EHGQLVQRVVSLEAERRLDRLQEDFIATVSHELLTPLGFIKGYATTLLREDAVWDDAT
                     RREFLSIIDEEADRLRELIDNLMDSSRLQAGALRMSFQPVRMDTFLKDLSFRAKSRDE
                     KQKINLELYSPDLIIQVDPARLAQVFDNLLSNANKYAPGAAISITLDRVNELAHIRVK
                     DDGPGIDPEHLPNIFKRFYRVPSNNTTVRGTGLGLFICRQIIRSHGGNISVESKPGDG
                     TSFDIYLPIDRVQTEEQISLRESN"
     gene            1585..2280
                     /locus_tag="AC16V7_10760"
     CDS             1585..2280
                     /locus_tag="AC16V7_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6257643.1"
                     /translation="MSTKILVVDDEPRYLRLIEANLASDGFTVIKAMNGQEAVEKVAS
                     HQPDLVLLDIMMPVLDGFGATERIREFSNVPIIVVTARGEEGARVRGLDLGADDYIVK
                     PFSATELLARVRAVLRRAQVSGGSFQQSIFNHGNLKIDFARAEVFRNDRVVFLSATEY
                     RLLLQFAHNLGQVLTSEQLLTNVWGQEYRDDKEILWVSISRLRQKLEDDPKNPIHIVT
                     RSGLGYTMPPMQD"
BASE COUNT          629 a          582 c          639 g          614 t
ORIGIN      
        1 ggcacctgtt agataatgct tgtaaattca ctcccagagg tggtcgcgtt aaactggagg
       61 tagaccagtc cgataatttt gttaaaatag cagtcacaga cacggggatt ggcattcctg
      121 cccatcggat gccagagatc ttcgagccat ttcaccaatt ggatggttcg tctacccgtc
      181 agtttagtgg aactggccta gggctggcat tatctcagag aattttgacg gcccatggca
      241 cacaaatcga agtccaatca caagaagaaa aggggtctcg atttgagttc ttgttgccac
      301 tcgcagaagt tgcgatgcaa atgtaaaacc agatatggtt gacgagacca gtaacccaac
      361 cgcacttcgc cctgaagaac tagaggcggt atactccatc agccgggctg tagctggggc
      421 tgtagatttg gatacagccc tggatgaaat tattcgttta acgcgctccg tatttatttt
      481 tgataacatg gtgctctata atttgcggag catcgacacg cttgagcccg catatgctcg
      541 cgccattggc cgggggcgct cccaagaggc cgagctggtt tggggcgagg ctaccgcctt
      601 ggaggcaatt caaaaaagtc aggtaatcat tctccaagag gagtcggggg atcaagaaga
      661 tcgtaccgga gtgcgttttt ctctcggttt accactcaga tcaggcaaag atatcaaagg
      721 agccctggtg tttatccgtt ttgggggtcc aacgtataaa cctgaccaga tacgcctggc
      781 agagtttatc gcaatgcacg tcgcccagtt gttagagcac ggtcaacttg tgcagcgagt
      841 ggtcagcctg gaggcagaac gccgtctgga ccggttacag gaagatttca tagccacggt
      901 atctcacgaa ttgcttacac cattaggttt cattaagggt tacgccacca cgctgttgcg
      961 tgaagacgcg gtctgggatg atgcaacgcg gcgtgaattc ttgagcatca ttgatgaaga
     1021 agcggatcgc ctgcgagaac tgatcgataa cctgatggat tcatccaggc tacaagccgg
     1081 cgcgctccgc atgagcttcc agcccgtccg catggatacc tttctcaaag atcttagttt
     1141 ccgagcaaag tctagggatg agaagcagaa aatcaatctc gaattatatt ccccagatct
     1201 gatcatccag gttgatccag cacggctggc tcaggttttc gataacttgc tgagtaacgc
     1261 caataagtac gccccaggtg cggctatctc gattacgctc gatcgagtta atgagctggc
     1321 tcatattcgc gtcaaagatg atggcccagg aattgatcct gagcatttac caaatatttt
     1381 caagaggttc tatcgtgtac ccagtaacaa taccacggtg cgcggcacgg gactggggct
     1441 gttcatttgt cggcaaataa ttcgctccca cggcggaaat atcagcgtgg aatcgaagcc
     1501 tggtgatgga acatcctttg atatatattt acccatagat cgtgttcaaa ccgaagaaca
     1561 aatttcactg cgggagagta actgatgagt acaaaaatcc tggtcgttga cgatgaacca
     1621 cgctatttgc gtctgatcga agctaacctg gcatcggatg gtttcacggt gatcaaggcg
     1681 atgaatggac aagaggcagt ggaaaaggtg gcttcccacc agccagacct ggtcctatta
     1741 gatatcatga tgcccgttct ggatggattt ggcgcgacag aacgtatccg ggaattctct
     1801 aacgttccca tcattgtcgt cactgctcga ggagaagagg gcgcgcgcgt gcgtggattg
     1861 gatttgggag cagacgatta cattgtcaaa cccttctcag ccaccgagtt gctggcaaga
     1921 gtgcgcgcgg tgctccgccg ggctcaagtc tccggtggta gttttcaaca gtcaatcttc
     1981 aaccatggca atctcaaaat tgattttgcc cgtgcagagg tattccgcaa tgatcgcgtg
     2041 gtgttcttat ctgctacaga atatcgcttg ctgttacaat tcgcgcacaa tcttggacag
     2101 gttctcacct cggagcaatt gttgaccaat gtttggggtc aggaataccg tgatgacaag
     2161 gagatcttgt gggtaagtat ctctcgattg cgccagaagc tagaggatga tcccaagaac
     2221 cccatccaca ttgtcactcg ctcggggcta ggctatacca tgccccccat gcaagactaa
     2281 atgacagata aggcaaatat ataatggtaa atcccctttc aatatctctt cgttcaaaga
     2341 aactcggtgt acttatccgc gacgcccgtc ttgcagcgcg taagagtatc gaagagtgtt
     2401 gtgcagtgat tgaggtatct ccagaagaat ttgaggccta tgaattaggg gacaaatctc
     2461 tatc
//
LOCUS       DBNHOH010000616         1597 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_934912, whole genome shotgun sequence.
ACCESSION   DBNHOH010000616 DBNHOH010000000
VERSION     DBNHOH010000616.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_934912"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..582
                     /locus_tag="AC16V7_10765"
     CDS             97..582
                     /locus_tag="AC16V7_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257644.1"
                     /translation="MTISNRIILVVALLTIVFGGVTLAFASGKDTIYAAEVGPEIEGT
                     WLATITIPDGPPPFPSLVTYARGGALTVTDSSGSPALGNVYQGSWAKTGPHKYVFTVM
                     GFLYDEEGVFAGYIRLHEIVKIEPGGNVYNGEASIEILDPEQNVVMTGSSISYAERIN
                     P"
     gene            911..>1597
                     /locus_tag="AC16V7_10770"
     CDS             911..>1597
                     /locus_tag="AC16V7_10770"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR6257645.1"
                     /translation="MDILAGLNPQQQQAVMAGLGPVLVLAGPGSGKTRVLTHRIGYLL
                     GAMGVRPYQILAVTFTNKAAREMQSRLTQLLEARPGGSPQSADSPQMGTFHGACARIL
                     RREAEHLPFQSNFVIFDADDQLNVVKTAIKDLGLDDKLYRPPGVHAAISNAKNELLLP
                     DDYPVQTYRDEVVARVYRRYQELLLASNALDFDDLLLWMTYLLDNEPAVRDKYARRYE
                     HVLVDEFQDTN"
BASE COUNT          351 a          426 c          435 g          385 t
ORIGIN      
        1 tgttattcgt aattgaaaat ctaatatcct gatgattgtc gtgtgctgtg agcacactat
       61 gtcaaattct gggtaactaa cttaggagag aatatcatga caatctcgaa cagaattatc
      121 ttggtagttg cgttactgac catcgtcttc ggaggggtca cattggcttt cgcttcaggc
      181 aaagacacaa tttatgctgc cgaagttggt ccggagatcg agggtacctg gttggcgacg
      241 attactattc cggacgggcc gccgccattt ccgtcactcg tcacttatgc tcgtggtggt
      301 gctctcaccg ttaccgacag tagcggatca ccagcattgg ggaatgttta ccagggatcc
      361 tgggccaaaa cgggtcccca caaatacgtc ttcactgtta tgggatttct gtatgatgaa
      421 gagggtgtct tcgcaggcta catcaggttg cacgagatcg ttaagatcga gcccggtggc
      481 aatgtttata acggagaagc atcgatcgag atccttgacc ctgagcagaa cgtggttatg
      541 actggtagct ctataagcta cgccgaacgt atcaatccat agcgactggc gtcctcttca
      601 cccagaatag aagcgggtgc cgagcccctg ggcattcctg acaacatttg tcgcgagtag
      661 gaatgcagat ttcgcgcact ccatccctct ccctgcctgg tcgaacgtca ggccagggtc
      721 gcctgtggga gagggacgat ttttatcagc cgaaggtatg tccaagtgat ggatgtgcaa
      781 aagttgcagg gaatgtctta tatattgtct gaacttcgga tcttgtctct taagcgcaaa
      841 ccttgtgtct gcattgagcg ggtggaaatt ctgttgacat gccccttccc tgacggtaga
      901 atgataaatc gtggatattc tcgctggcct caacccccaa cagcagcagg cggtaatggc
      961 tgggctgggt cccgtgctgg tgctggccgg gcccggctct ggcaagacgc gcgtgctcac
     1021 ccaccgcatc ggttacttgc tgggcgcgat gggtgtgcgc ccctaccaga tcctggctgt
     1081 aaccttcacc aacaaggccg cccgagagat gcaaagccgc ctgacccagc tcttggaggc
     1141 gcgacccggc gggtcgcccc aatccgctga tagccctcag atggggacct tccatggcgc
     1201 ttgtgcccgc atcctacgca gggaggcgga gcacctgcct tttcaatcca actttgtcat
     1261 ctttgacgcc gatgatcagt taaatgtcgt aaagactgct attaaagacc ttggtctgga
     1321 tgacaagctc taccgcccgc caggcgtgca tgcagccata tccaacgcca agaatgagct
     1381 gctgctcccc gacgattacc ccgtgcaaac ttatcgtgat gaggtggttg cacgcgtcta
     1441 ccgtcgctac caggagttgc tgctggccag caacgccctg gatttcgatg atctgctcct
     1501 gtggatgacg tacttgctgg ataacgaacc agcggtgcgc gataaatacg cccgccgcta
     1561 cgagcacgtg ctggtggatg aattccagga taccaac
//
LOCUS       DBNHOH010000617         6738 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_870133, whole genome shotgun sequence.
ACCESSION   DBNHOH010000617 DBNHOH010000000
VERSION     DBNHOH010000617.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6738)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6738)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6738
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_870133"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1106
                     /locus_tag="AC16V7_10775"
     CDS             <1..1106
                     /locus_tag="AC16V7_10775"
                     /inference="COORDINATES: protein motif:HMM:NF013741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR6257646.1"
                     /translation="GILTGAFSGATIAEELEAYLGHTQIPLLSAYGEVISVSVVVIVI
                     TFLSLIVGELAPKQIGLNNPERIASIVAPIMRTISRLTSPLVHLLSFSTSLVLRLIGM
                     RRTTDPEVTEDEIKLMIEQATAGGIFYPEEEEMVEQVFRLADRPIVAQMTPRTEIVWL
                     DLENPIEAIRETLTAARYSYYPVGRGGVDNLLGVVHAKDLLAQCVSGKSLNIEEKIIQ
                     PQYLPEGMTILETLKRFRESRTQIAFIIDEFGGIQGLTTITNILEAIAGDFPDAIDAA
                     EQEIVVRKDGSYLLNGMLDIDEFKDLFRIAALPGEEGNSYQTLGGFVMMFLGRIPSAG
                     DYFEWENLRIEIVDMDGLRVDKVLVTPSTVSLQ"
     gene            complement(1137..2192)
                     /locus_tag="AC16V7_10780"
     CDS             complement(1137..2192)
                     /locus_tag="AC16V7_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016877390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HIR6257647.1"
                     /translation="MNTLPSITRIKTLPLPPHFDPGKVAQVWRVPYQALAESASQWAR
                     QHNLQPAAEDHFRVALIAVDMQNTFCLPDFELFVAGRSGMGAVEDNRRLCEFIYRNLG
                     VLTRIIPTMDTHQAMQIFHPIFLVNEQGEHPAPLSLISYQDVEQGRWRFNAAIAPILG
                     IDAAYGQEHLLHYTRVLKEQGKYDLTIWPYHAMLGGIGHALVSSFEEAIFFHSIARQA
                     QPAIETKGQLAISESYSAIGPEVLEDWRGEQIGQKNPRFLGLLDEFDAVVIAGEAKSH
                     CVAWTVDDLLSQIQAYNPLLAHKVYLLEDCTSPVVVPDIVDYTGSADEAFQRFAEAGM
                     HIVRSTDPIASWPGMAG"
     gene            complement(2398..3219)
                     /locus_tag="AC16V7_10785"
     CDS             complement(2398..3219)
                     /locus_tag="AC16V7_10785"
                     /inference="COORDINATES: protein motif:HMM:NF015213.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HIR6257648.1"
                     /translation="MLPSFLLSLREGLEAALIIGIVLGALRKIHRQDLSPVVWGGTLS
                     ASAISVIVALALYAVGASLEGSAEGVFEGITVFIAAGVLTWMIFWMHRQSRTIKGELE
                     LGVRHAVSKTGVRALFLLAFLAVLREGVELALFLTASTFAINAQHTILGAVLGMGTAI
                     LLGWSLFTSTVRLDLRRFFQITGALLILFAAGLVAYGVHEFNELGWIPSLIEHVWDIN
                     FILDEKSTLGLMLKALFGYNGNPSLSEVLAYIGYFAAILVGLRRSQEPVASVQNA"
     gene            complement(3249..3698)
                     /locus_tag="AC16V7_10790"
     CDS             complement(3249..3698)
                     /locus_tag="AC16V7_10790"
                     /inference="COORDINATES: protein motif:HMM:NF013629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR6257649.1"
                     /translation="MPDNQDTFIDTLHDHGLRLTPQRELVLQILREASEHLDAEGIWQ
                     NARQYDQDINLATIYRNLNVLSQIGLIQQSFLGEGQKRGYYELIDKPFHYHFACLRCG
                     KVLELLSPPMAQAQQEMERHYGVRIIDAHYKFEGLCPDCMASHANDE"
     gene            4115..6184
                     /locus_tag="AC16V7_10795"
     CDS             4115..6184
                     /locus_tag="AC16V7_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR6257650.1"
                     /translation="MNKYADLEIGLHRRELDTYSVDFRFSQPDSETDVRPGRGDAQID
                     LKALQKFLPGSADYSQALTSSLFTDPAVLTAFSQAVASVQTSGSALRVRLLIGSSAPE
                     LHGIHWETLRNPQTDAPLCTDENILFSRYLSSLDWRPVHLRPKGDLKALVVVANPSNL
                     SDYQNLAPVDVEGETTRARENLGPIHVTTLPPSPSDTAPGSRASLPNLIARLRDENPD
                     ILYLVAHGALTRDEPKIWLEDEDGKAAVTSGTELVTRLRELSERPLLVVLASCQSAAH
                     PTYGEALTALGPRMAEAGIPAVIAMQANVSMKTIAEFMPVFFAELQKDGQIDRALSVA
                     RGTVRQRPDYWMPALFMRLKSGRIWYTPGFGEERGAFEKWPSIIRTISRGLSTPILGP
                     GLYETVLGSPREIARRWAEVYHYPMEPHERESLPQVAQFLAVNQYDLAPFDELEEYLK
                     KDIRSRFGDNLPDELRANGASLDQLIEAAGAIRRQQSEVEPHKVLASLPLPVYITTNL
                     NNLMGSALKEAGKEPQIVLCPWNDYVEELQADFDPGYKPTVERPLVYHLFGRLSEPES
                     VVLTEDHYFDYLIGVTRNKEIIPSVVRSALVNSALLFLGFQLDDWQFRVLFRSVRAQQ
                     GGELLGRYPHIAVQLEPEEGRLLEPERARKYLESYFSAGSNISLFWGRAEDFVKELHS
                     RWKARSA"
     gene            6194..>6738
                     /locus_tag="AC16V7_10800"
     CDS             6194..>6738
                     /locus_tag="AC16V7_10800"
                     /inference="COORDINATES: protein motif:HMM:NF044287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6257651.1"
                     /translation="MTITNTRPNPYVGPRAFRTGEALYGRGRELHELLNLLIAERIVL
                     LHSPSGAGKSSLVYAGLIPRLEEEGFQVLPVARVNQDLPASLGSDERFNRYAFSTVVS
                     LDEALPEKKRLPLEELATISLPDYLERFKAQNLTTVGDTDDLPPMEAEVLIFDQFEEV
                     LTVAPTDLEGKAAFFAQLGAA"
BASE COUNT         1683 a         1809 c         1685 g         1561 t
ORIGIN      
        1 tgggtatact gactggtgca tttagtgggg cgaccatcgc agaagaacta gaggcctatt
       61 taggtcacac gcagatacca ctcctgtcgg catacggtga ggtaatcagt gttagcgtgg
      121 tagtgatcgt aattacgttt ctgtcgctta ttgtgggtga actagccccc aaacaaatcg
      181 gattgaacaa cccagaaagg atcgcctcaa tcgtcgcccc aattatgcga acgatctcac
      241 gtctgacatc gcccttagtc cacctgctca gtttttcaac gagcctggtc ttacgcctca
      301 tcgggatgag gaggacaact gatccggaag tcacggagga tgagatcaag ctgatgattg
      361 agcaagcgac ggcagggggg attttttacc cagaagaaga ggagatggtc gaacaggttt
      421 tccgcttagc cgaccgccca attgtggcac aaatgacacc ccgtaccgag attgtctggc
      481 tggatttaga gaatcccatc gaagcgatcc gggagacgct tacagccgcc cgatattcgt
      541 attaccccgt ggggcggggt ggcgtggata acttgctggg tgtggtgcat gcaaaagacc
      601 tcctggcgca atgcgtgagt gggaaatctc ttaacataga ggaaaaaata attcaacccc
      661 aatacttgcc cgagggcatg accattttag agactttgaa gcggtttcga gagagccgca
      721 cccagatcgc ctttatcatc gacgagtttg gcggaatcca ggggctgacg accatcacca
      781 acatcctgga ggcaattgcc ggcgatttcc cggatgccat cgacgcagcc gaacaagaga
      841 tcgtagtccg aaaggacggt agctacctac tgaatggaat gctagacatc gatgagttca
      901 aagatctgtt ccgtatagct gcgctaccag gcgaggaagg taactcttat cagacgctcg
      961 gcgggtttgt gatgatgttt ttaggccgta tcccttccgc gggagactac ttcgaatggg
     1021 aaaatctgcg tatcgaaatt gtcgatatgg atggccttcg ggtggataag gtattggtaa
     1081 cacctagcac ggtctcacta caataaccca tcaagagact ataggaaatc taagctttac
     1141 cccgccatcc ccggccagct tgcgatggga tcggtggagc ggacgatgtg catacccgct
     1201 tcagcgaagc gctggaaggc ttcatcggcc gatccagtgt agtccacgat atccggcacc
     1261 accaccggcg aggtgcaatc ttccaataaa tacaccttgt gcgccaggag cggattgtag
     1321 gcctggatct ggctcaacaa gtcatcgact gtccatgcca cacagtggct ctttgcctct
     1381 ccggcaatta ccacggcatc gaattcgtcc aaaaggccga gaaaacgggg attcttctgg
     1441 ccgatttgtt caccgcgcca gtcttcaagc acctccgggc caatcgcaga atagctctcg
     1501 gaaatggcta actgtccttt ggtttcaatc gctggctgtg cctgccgggc aatggagtga
     1561 aagaagatcg cctcttcaaa agatgaaacc aaggcatgac caattccccc cagcatggca
     1621 tgatatggcc agatggtcaa atcgtacttc ccctgctcct ttagcacccg agtatagtgc
     1681 aacaagtgct cctgtccgta ggctgcgtct atccccagga tgggagcaat cgccgcattg
     1741 aacctccatc gtccttgctc tacatcctga taagagatta atgagagcgg cgcaggatgc
     1801 tcaccctgct cgttcaccaa gaaaatgggg tggaagattt gcatagcctg gtgtgtatcc
     1861 atagtcggga taatgcgagt taataccccc aggttgcgat aaatgaactc acacaggcgg
     1921 cgattatcct caacggcccc catacctgag cgaccagcga caaacaattc gaagtccggg
     1981 aggcaaaaag tgttctgcat atcgactgcg atcagggcaa ccctgaagtg atcttcagct
     2041 gcgggttgca ggttatgctg gcgtgcccat tgcgaagccg attctgccag cgcctggtaa
     2101 ggcactctcc atacttgggc taccttccct gggtcgaagt gcggtggcaa tggcaaagtt
     2161 ttaatccgag tgattgatgg cagggtattc atgctacctt cctgagaatt caattagtgt
     2221 ataaactaca ctaattatac cagaatcaat gcgcttcgtc aagctaaatt tacgtcatct
     2281 aggttaaaaa atcacagcgg ggttattgcc cgctgtgatt tttccggaag atattcattt
     2341 ggtgaggatt agcctttcga ccgtttaagt ctcaataagc tgaggtcgcc attcacatta
     2401 tgcattctgt accgatgcca ctggctcctg gctgcgtcgc aagcccacca gaatggcagc
     2461 gaaatatcca atatatgcca acacctcaga cagggatggg ttgccattgt atccgaagag
     2521 ggctttcaac atcaacccca gggtggattt ctcgtctaag atgaaattaa tatcccatac
     2581 atgttcaatt agggatggga tccaaccaag ctcattgaat tcgtggaccc cataagccac
     2641 caacccggct gcaaacaaaa tcagcagagc accggtgatc tgaaaaaatc tgcgcaaatc
     2701 tagccgaact gtggatgtaa acagcgacca gcctaataaa atcgccgttc ccattcccag
     2761 cactgcaccc aaaattgtgt gctgggcgtt tatggcaaac gtggatgcgg tcaggaagag
     2821 tgctaactca acaccctcgc gtaagactgc caggaatgcc aataaaaaca atgcacgcac
     2881 acccgttttc gatacggcat gacgcacacc cagctccagc tcacccttga tagtacggga
     2941 ctggcgatgc atccaaaaga tcatccaggt tagcacaccc gctgcaatga acacggtgat
     3001 tccttcaaat acaccctcgg ctgaaccctc tagcgaagcg ccaaccgcat ataatgccag
     3061 agcgacaatc acgctgatag ccgatgcgct tagcgtaccg ccccatacca cggggctcaa
     3121 gtcttggcga tgaatttttc gcaaggcacc caaaacaatg cctataatca gggcagcctc
     3181 caagccctcg cgtaatgaaa gaagaaaact aggaagcacg acaatcctcc ttattaggga
     3241 ttaggtagtt actcatcatt tgcatgggat gccatacaat ctggacaaag accttcgaac
     3301 ttatagtgtg catcgatgat acgcacgcca tagtggcgtt ccatttcttg ctgagcttgc
     3361 gccatcggtg ggctgagcaa ttcaagcacc ttcccgcaac gaaggcaggc gaagtgatag
     3421 tgaaatggct tatcaattaa ctcgtaataa cccctcttct gcccttctcc caaaaagctc
     3481 tgttgaataa gcccaatttg ggataagaca tttagattac ggtaaattgt agccaggttg
     3541 atatcttgat catactggcg ggcgttttgc cagatcccct ccgcatccaa atgctcagat
     3601 gcctcacgta gaatctgcaa gaccagctct ctttgcggtg ttagccgcaa cccgtgatca
     3661 tgcaaggtat cgatgaaggt gtcttggttg tcaggcatag tattggtgcc gccccttatt
     3721 gcaaatgatt tgcaattgca ttctatcaca gatataatct ctatccactg atagatgtca
     3781 tcaatgtgtt ggtaaatcgt catacttctc ttgtcggcga gatcaaaccc cgtttcatca
     3841 atgattggtt aggaacaaaa atgtttttaa gtcgcgttct aatcaaataa aggggtgata
     3901 caataataac ctggggatca ccctttatca tgcttgcctt ttttgattat ttcgagtaaa
     3961 tttataaata ttatgtaaat actttgatct gatactcaaa gctctacatt gaaagactgc
     4021 aaatttttca cacctttata caccgaatta cccggaaacg tgatcttagg ggagacaaag
     4081 ctcaaatctc taaacgaggt tttttggaga aaacatgaat aaatacgctg acctcgaaat
     4141 cggtctgcat cgccgggaat tagatacata cagcgttgac ttccggttca gccagccgga
     4201 tagtgaaacc gatgtccgcc cagggcgtgg tgacgctcag atcgacctca aagcgctgca
     4261 aaaatttctt ccgggaagcg ccgactacag ccaggcgctc accagcagcc tatttaccga
     4321 cccagccgtg ctgaccgcct tctcccaggc ggttgccagc gtacagactt ctgggtcagc
     4381 gctgcgagtt cgtctgctca tcggctccag cgctccagag ctgcatggta ttcactggga
     4441 aacattacgc aatccgcaga ctgacgcgcc gctttgtacc gacgagaata tccttttctc
     4501 ccgctatctg agtagcctgg actggcgacc ggtgcacctg cgtcccaaag gcgatctgaa
     4561 ggctttggtc gtcgtcgcca accccagcaa tctctcggat tatcaaaatc tagccccggt
     4621 cgatgtagaa ggcgaaacca cccgggcgcg agaaaaccta ggccccatcc atgtgacaac
     4681 cctcccccct tctccttctg atacagcgcc aggatccagg gcatcgttgc caaacctgat
     4741 cgcacgcctg cgggatgaga atccggatat tctctatctt gtggctcatg gggcgctcac
     4801 gcgcgacgag ccaaaaatat ggctggagga tgaagatggc aaagccgcag tcacttccgg
     4861 caccgaattg gttacacggc tgcgcgagct ttccgaaaga cccttgctgg tggttctagc
     4921 ctcctgccag tcagctgcac atcccacata tggcgaagcc cttactgcac tgggtccccg
     4981 gatggccgag gctggcatac cagccgtaat agccatgcaa gccaacgtca gcatgaagac
     5041 catcgccgag tttatgcccg tctttttcgc cgaactgcag aaagatgggc agatcgaccg
     5101 cgctttgtcc gtagcgcgcg gaacagtgcg ccagcgcccc gattactgga tgccagcctt
     5161 atttatgcga ctgaagagcg ggcgtatttg gtacacgccc ggttttgggg aagagcgcgg
     5221 agcttttgag aaatggcctt ccatcatccg caccatcagc cgaggcctga gtaccccgat
     5281 cctcggaccg ggattgtatg aaactgtgct tggctcgccg cgcgagattg cccggcgctg
     5341 ggcggaagtt taccactatc cgatggagcc acacgagcgc gagtccctac ctcaggtagc
     5401 ccaatttctt gccgtaaacc aatacgacct ggcgcctttc gacgagctgg aagaatatct
     5461 caagaaggat atacgaagcc ggtttggcga caacttacca gacgagctgc gcgctaacgg
     5521 cgcctctctg gaccagctta tcgaggcagc tggggccatt cgccgacaac aatcggaggt
     5581 agaaccacat aaagtgctcg cctccctgcc cctcccggtt tacatcacaa ccaacctgaa
     5641 taacctgatg ggctcagccc taaaggaagc gggcaaagag ccccagatcg tgctctgccc
     5701 gtggaatgat tacgtcgaag agctccaagc agactttgat ccaggttaca agccgaccgt
     5761 ggagcgcccg ctggtctacc atctttttgg tcggttaagc gaaccagaat ctgtggtgct
     5821 taccgaagat cactatttcg attatctcat tggggttacc cgcaacaagg aaatcatccc
     5881 cagcgtggtg cgcagtgccc tggttaattc ggcgctcttg ttcctaggat ttcaactcga
     5941 cgactggcaa tttcgcgtac tcttccgcag tgtacgcgcc caacagggtg gtgaattgct
     6001 cggacgttac ccgcatatcg ctgtgcaact tgaacccgag gaaggccgcc tattagaacc
     6061 cgagcgcgcc cgaaaatatt tagaaagcta tttttctgct ggctccaaca tcagtctgtt
     6121 ttgggggcga gccgaagact ttgtcaaaga actacattcg cgctggaagg cgcgttcagc
     6181 ctgagatgaa gccatgacaa taacgaatac ccgtcctaac ccatatgttg gtccgagagc
     6241 ctttcgcaca ggcgaagcgc tttatggtcg cggtcgcgaa ctgcacgagc tcttgaactt
     6301 gctcatcgcc gagcggattg tcttattaca ttcaccctct ggagcaggca aaagctcgct
     6361 ggtgtatgcc ggcttgatcc cccgtctcga ggaggagggg ttccaggtgc tacctgtcgc
     6421 gcgcgttaac caggacctgc ccgcatcgct aggcagcgat gagcgcttca atcgctatgc
     6481 ctttagcacc gttgtctcgc tggacgaggc tctgccagag aagaagcggc ttcctttaga
     6541 ggaattggcg accatctcat tgcccgatta cttggagcgt ttcaaggctc aaaacttaac
     6601 cactgttggt gatacagacg atctgccacc catggaagcc gaggtgttga tatttgatca
     6661 gtttgaagag gtgctgactg tcgcccccac tgacctagaa ggcaaagccg ccttttttgc
     6721 acagctaggt gcagcctt
//
LOCUS       DBNHOH010000618         2008 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1190968, whole genome shotgun sequence.
ACCESSION   DBNHOH010000618 DBNHOH010000000
VERSION     DBNHOH010000618.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1190968"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..1296
                     /gene="coaBC"
                     /locus_tag="AC16V7_10805"
     CDS             64..1296
                     /gene="coaBC"
                     /locus_tag="AC16V7_10805"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIR6257652.1"
                     /translation="MANPLKDKHIILGVTGSIACYKAVELASKLAQAGALVDVILTQA
                     ATQFVTPLTFQSVTGRRAFVEADLWGSEGHVLHISLGHVAELLVIAPASANTIAKLAH
                     GIADNFLTVTALAASCPLLIAPAMDGGMYAHPATQTNLKTLQERGALILGPVEGHLAS
                     GLQGVGRMMEPGELLGHIRLALAQTGLLHGRRVIVTAGGTHEPIDPVRAISNRSSGKQ
                     GFALAQAALDLGAQVTLITAPVSLPTPVGAQRQEVLTAQDMLEAVSEAIPHADALIMA
                     AAVADFRPADPAGQKIKREGGLPEIQLENTPDILQAVARYKSDTGYPRVTVGFAAESQ
                     ALLENARKKLEKKSLDLIVANDISASDTGFAVDFNRVTLLDAQGGVEHLPRMSKTDVA
                     QIVMQRVVAILNSLHSEL"
     gene            1293..1610
                     /locus_tag="AC16V7_10810"
     CDS             1293..1610
                     /locus_tag="AC16V7_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009555946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HIR6257653.1"
                     /translation="MIVHICQLGAWQAAQARGEYCPPSLDDEGFIHCSKPEQVLHVAK
                     RFYRDVPDLILLWIDTEKVRPEIRWEEADGEVFPHIYGALNTDAVVSVSELGPGMDLN
                     PPF"
     gene            complement(1619..>2008)
                     /locus_tag="AC16V7_10815"
     CDS             complement(1619..>2008)
                     /locus_tag="AC16V7_10815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6257654.1"
                     /translation="NLHFLPPVPKGEMPRALAAADACIAILKPIEMYKTVYPNKVFDY
                     MAAGRPVILAIDGVIRQVVEVAGSGVYVPPGDAKALANAIRELAANPAMRQSMGLAGR
                     RCVEEAFSRELLAEKMAEIIEEMRDRD"
BASE COUNT          454 a          586 c          540 g          428 t
ORIGIN      
        1 gctaaaggaa tacttcggca tctttattta caagctgcgt ggttggttgt aatataagca
       61 tatatggcta atccactcaa agacaaacac atcatcctcg gcgtcacagg ttcgattgcg
      121 tgctataaag ccgttgagct ggcgtccaag ctggcccagg ctggtgcgct ggtggacgtc
      181 atccttaccc aggcagcgac tcaattcgtc accccgctca ctttccagtc agtcactgga
      241 cgacgcgctt ttgtggaagc cgatctatgg ggcagcgaag gtcacgtgct tcacatcagc
      301 ctaggccatg tcgctgaact tctggtaatc gcacccgcct cggcaaatac catcgccaag
      361 ctggcgcacg gcatcgccga taatttcctg acggtgactg cgctggcagc cagctgccca
      421 ttgctcattg caccagctat ggatggcggg atgtacgccc acccggcgac tcaaaccaat
      481 ctgaagacct tgcaggagcg gggcgctctc attcttggtc cagttgaggg ccacctggca
      541 tccggattgc aaggagtcgg gcgaatgatg gagcccggag agttgcttgg tcatatccgc
      601 ctggcactgg cgcagaccgg tctgcttcat gggcgcagag tgattgtcac tgcaggcggc
      661 acgcacgagc ccatcgaccc ggtaagagct atcagcaatc gctcatcagg caaacagggc
      721 tttgccctgg cccaagctgc cctggatttg ggggcgcagg ttactttgat aacagctccc
      781 gtctctctgc ccacgccagt gggggcgcag aggcaggagg tgctcacagc ccaagacatg
      841 ctggaagcgg tatcagaggc tattccgcat gccgacgcac tgattatggc agccgctgtg
      901 gccgattttc gacccgctga ccccgccggg caaaagatca agcgcgaagg tggtcttcca
      961 gagattcaac tcgaaaacac gcctgatatc ttgcaagctg ttgcccgcta taaatccgac
     1021 accggttacc cgcgggtgac agtaggtttc gctgccgaaa gccaggcttt actggaaaat
     1081 gcccgcaaga agctggaaaa aaagagcctg gatttgatcg tcgccaacga tatctccgcc
     1141 agcgataccg gcttcgcggt ggacttcaac cgcgtgacct tgctagacgc ccaaggtgga
     1201 gtagaacacc tgccccgcat gagcaagaca gatgtagcgc agattgtaat gcaacgggtt
     1261 gtggcgatat taaattcttt gcattcagag ttatgatcgt gcatatctgc cagctcgggg
     1321 catggcaagc agcccaagcc cgcggagaat actgcccacc ctccctggac gacgaaggtt
     1381 tcatccactg ctccaaacca gaacaagtct tgcatgttgc gaagcgattt taccgcgatg
     1441 tacctgattt gatcttatta tggatcgaca ccgaaaaagt gcgcccagag atccgctggg
     1501 aagaggcaga tggggaggtt ttcccccata tttacggggc actcaataca gacgcagtgg
     1561 tctctgtgag cgaactgggc ccaggtatgg atctaaatcc acccttctaa aacctctatt
     1621 aatccctatc cctcatctcc tcgataattt cagccatctt ctcagccagc aactcgcgcg
     1681 aaaaggcttc ctccacgcag cgccgaccag ccagccccat cgactgtctc atggcggggt
     1741 ttgcagccaa ctcgcggatg gcgttcgcta aggctttcgc atctccgggt gggacgtaaa
     1801 caccgctgcc tgccacctcg acaacctgcc ggattacacc atcgatcgcc aagatcaccg
     1861 gccggccagc cgccatgtaa tcgaagactt tatttggata gaccgttttg tacatctcaa
     1921 taggtttgag aatggcgatg caggcgtctg ccgccgccag cgcccgaggc atctctccct
     1981 ttggcaccgg cgggaggaaa tgcaagtt
//
LOCUS       DBNHOH010000619         2896 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1126154, whole genome shotgun sequence.
ACCESSION   DBNHOH010000619 DBNHOH010000000
VERSION     DBNHOH010000619.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1126154"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="AC16V7_10820"
     CDS             complement(<1..481)
                     /locus_tag="AC16V7_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HIR6257655.1"
                     /translation="MESIQDKALRKADEASQRFRQAEQGRDEKLSMLSFATIDTVDDI
                     GAVEDKERLDARKALLNTNDGLALERILGKSDLVSINYLEIGLKAARAVCRIQVRDEL
                     GRMRGFGTGFMVSPSLMLTNNHVLESPSMARRSLADFNFQDDANFIPLDTKTFPLDAD
                     "
     gene            complement(677..1093)
                     /locus_tag="AC16V7_10825"
     CDS             complement(677..1093)
                     /locus_tag="AC16V7_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR6257656.1"
                     /translation="MNDDVIRQILTINDVIATVGLSANPAKTSHGVAQYLIKQGYDLI
                     PVNPGVDEVFGRKAYPDLISIPRKVDVVQIFRPSSEVPPIVDQAIQIGAKVVWMQVGI
                     INHEAAETARAAGLQVVMDRCLRQEHIRLVGHIMDM"
     gene            complement(1090..2490)
                     /locus_tag="AC16V7_10830"
     CDS             complement(1090..2490)
                     /locus_tag="AC16V7_10830"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6257657.1"
                     /translation="MNAELLQKAPLLASLSPEDLDWLATLSINVVVQAGETLIREGDE
                     GDSAYLILDGELEVSKRSGDRDVMLSVRRSGEVIGEMALLTQAPRMATVRALQETHLC
                     KIPAEAFEQLLRTHPSATLVILRTVTTRLKNTEALLHQREKMASLGTMAAGLAHELNN
                     PAAAAQRSVAQLQEALVNWQKLNFSLQQSASDERTSMLIDQLHAELLRRSAVPASLDP
                     LARLDQEQSLQEWLEERGMDDAWQLTQPLVLAGWSSDELNSFTGSIPSEQFIQLIRWL
                     AAGCLVYSLLDEVHQSTSRISEIVKGVKNYTYLDQAPIQEVDVHEGLENTLLILRHKL
                     KTGVNVIRQYAQNLPRIEAYASELNQVWTNIIDNGIDAMQGQGELILRTYAKNDHVVV
                     EIQDSGPGIPPDIQPRIFDPFFTTKPPGSGTGLGLNIAYGIVVNKHCGEIHLSSKPGA
                     TCFQVILPVRLGKDKQ"
     gene            complement(2499..>2896)
                     /locus_tag="AC16V7_10835"
     CDS             complement(2499..>2896)
                     /locus_tag="AC16V7_10835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6257658.1"
                     /translation="IDIYLNTDLIEVHGEGKLEEIVVKNNRIDELQTLKGAAMFVFIG
                     VRPQSQFVADLVARAEKGYVLTGPDLLVEGKRPANWPLDRDPYLLETSVPGIFAAGDV
                     RYGTTHRVSAATSEGGLAVALIRQYLKTI"
BASE COUNT          659 a          817 c          740 g          680 t
ORIGIN      
        1 gatccgcatc gagaggaaaa gtcttggtat caagcgggat gaagttggca tcatcctgaa
       61 agttaaaatc ggccaggctg cgccgtgcca tggatgggct ttccaacacg tggttattgg
      121 tgagcataag gctgggcgag accatgaaac cggtgccgaa gccccgcatt cgccccagct
      181 cgtcacgcac ttgaatgcgg catacggcgc gcgccgcctt cagcccgatc tccaggtaat
      241 tgatactcac cagatcgctc ttgcccaaga tgcgctccag ggctaggcca tcattcgtgt
      301 tgagcaacgc cttacgggcg tcaaggcgtt ctttgtcctc caccgcaccg atatcatcca
      361 cagtatcgat cgtggcgaag gaaagcatgc ttagcttttc atcgcgccct tgctctgcct
      421 gccgaaagcg ctgcgaggct tcatcggctt tgcgcaaggc tttgtcttga atagattcca
      481 tgacaatctc ctagggtaaa taatagaata tgctacttac atctacgccg cacccctgaa
      541 tagtctggag gatgtgattg tattcacggt gttcaataat ttccccttcc cagggaagag
      601 gggacagcct gcctaccaga gaggcagctc aggcagagat gaaggaaata agtaactaaa
      661 tgatctctct ataccatcac atatccataa tatggccaac caggcggatg tgttcctggc
      721 gcaagcagcg atccatcact acctgaaggc cggcggcacg cgctgtctca gcggcttcgt
      781 gattgattat acccacttgc atccacacaa ctttcgcccc aatttgaatc gcctggtcga
      841 caatcggggg tacttcgctc gacggcctga agatctgcac cacgtctact ttacgcggga
      901 ttgaaatcag gtcagggtag gccttacgac caaatacctc gtcgactccg gggttgaccg
      961 ggatcaaatc gtatccttgt ttgattaagt attgggcaac cccatggctg gtcttagcag
     1021 gattggccga taatcccaca gttgcgatta catcattgat tgtcaatatt tgtctgataa
     1081 cgtcatcatt cattgtttat cctttccgag acgtaccggt aatattacct ggaagcaggt
     1141 cgcaccgggc ttggatgaca ggtgaatctc accgcaatgc ttgttgacca caataccgta
     1201 agcgatgttc aaccccaggc cagtcccgct gccaggaggt tttgtggtaa agaacgggtc
     1261 aaaaatgcgc ggctgaatat cggggggaat gcccggcccc gaatcctgta tctcgaccac
     1321 cacatgatca ttttttgcat aggttctcag aatcagctca ccctgcccct gcatggcatc
     1381 gataccgtta tcgatgatgt tcgtccacac ttggttaagt tcgctggcat atgcctcaat
     1441 acgaggtaaa ttctgcgcat actgccggat gacatttacc cccgtcttta gcttatggcg
     1501 gaggatcaac agtgtgttct ccagaccttc gtggacatcc acctcctgga tcggcgcctg
     1561 gtctagatac gtataatttt tgaccccctt gacgatctcc gagatgcgac tggtgctttg
     1621 gtgcacttca tctaacaggc tgtacaccag gcagccggca gccagccagc ggatgagctg
     1681 gataaattgt tcagagggaa tggaaccagt aaaggaattc agctcatcag aagaccatcc
     1741 agccagaaca aggggctgcg tcaattgcca ggcatcatcc ataccgcgct cttccagcca
     1801 ctcttgcagg gattgttcct ggtcgagccg cgccagtggg tcaaggctgg ctgggacagc
     1861 actgcggcgc aatagctcgg catgcaattg atcgatgagc atagaggtgc gttcatcact
     1921 cgcactctgc tgcaagctaa agttcagctt ctgccaattt acgagcgcct cttgtaattg
     1981 ggctacactg cgctgagcag ccgcagcagg attgttcagc tcatgggcaa gaccagccgc
     2041 catcgttccc aaggaggcca ttttctctcg ctggtgcagc aaggcctcgg tattcttcag
     2101 tcgggtggtg actgtacgca atattaccag cgtagctgaa ggatgagtcc tgagcaactg
     2161 ttcaaacgcc tcagccggga tcttgcacaa gtgggtttcc tgcaaggcgc gcacagttgc
     2221 catgcgggga gcctgcgtga gcaaagccat ctccccaatc acctctccag accggcgaac
     2281 cgataacatg acatcccggt ctccagagcg tttgctcacc tcgagctctc catccaggat
     2341 gagataagct gagtcccctt cgtcgccttc acgaattaac gtctcacctg cctgcacaac
     2401 gacgtttatg gataacgtcg ccagccaatc aagatcctct ggagaaagac tagccagcaa
     2461 gggcgctttt tgcaggagct cagcgttcat cacacgacct aaatggtttt caggtattgc
     2521 cggatcaagg ccaccgccag gccgccttcg ctggtggcag ctgaaacgcg gtgggttgtg
     2581 ccgtaacgaa catctcccgc cgcaaaaata cccgggacgc tggtttccag caggtatgga
     2641 tcgcggtcca gaggccaatt tgccggccgc ttgccctcga caaggagatc cggcccggtg
     2701 agcacgtagc ccttctcagc ccgcgccacc aggtcggcaa cgaactggct ctgcggtctc
     2761 accccaatga acacaaacat ggctgcccct ttgagggttt gaagctcgtc aatgcggtta
     2821 ttcttcacca cgatctcttc cagttttccc tctccgtgca cttcgatgag gtctgtgttg
     2881 agatagatgt cgatct
//
LOCUS       DBNHOH010000620         4032 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_935141, whole genome shotgun sequence.
ACCESSION   DBNHOH010000620 DBNHOH010000000
VERSION     DBNHOH010000620.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_935141"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="AC16V7_10840"
     CDS             complement(<1..586)
                     /locus_tag="AC16V7_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257659.1"
                     /translation="MAQKKGQIFLCNLVLLSLLFNFPAMPFFFSVPELAPSSITLQQS
                     PVLHANAPINPDQAIALAVNDIEATDDGYILRHPRHIATFNSDGLQFTPRPAGPQWAW
                     QLGFVGTSETALPGVNTGVVQPVNEHPGVVSYDRGGLFEQYVAQKDSIEQQFFIPSPL
                     PLNGEDLVIAGAVHTEAEFEQVDEGWRWHTSTGGI"
     gene            933..1358
                     /locus_tag="AC16V7_10845"
     CDS             933..1358
                     /locus_tag="AC16V7_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012899484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HIR6257660.1"
                     /translation="MPVKTFNIPLSTRGHADILDITDKVAGCVAESGLQDGIVTIFCP
                     SSTSALTTIEYERGCISDLRRLFDEIINPNRHYDHDQAWGDGNGHSHVRAALLKASLT
                     VPFVRARLTLGTWQQIIYVDFDTRSRRRELVVQIIGEVG"
     gene            complement(1397..2236)
                     /locus_tag="AC16V7_10850"
     CDS             complement(1397..2236)
                     /locus_tag="AC16V7_10850"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF021507.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HIR6257661.1"
                     /translation="MTFTILGGLVGFNWLMFIPRSQPKPANQALFQGITYAREVRNSS
                     RPMVAHVVTVNLRAPGISLLVTPGDPKAERPLKARTTSKFLDQFGVQIAINGDGFTPW
                     YSNNILDYYPRSGDAVEPIGFAASDGKVYSDNLHEQPVLYFTRNNQARFGTPPGKIIN
                     AISGNLMLVQDGKVLPSLEGTREPRTAIGLDKRGRSLIIVVVDGRQPGYSEGATLEEL
                     ASILVAHGAHTAMNLDGGGSSTLVIEQGGKATLLNSPINHGIPGWERPVGNHLGVFAE
                     QTK"
     gene            complement(2287..3102)
                     /locus_tag="AC16V7_10855"
     CDS             complement(2287..3102)
                     /locus_tag="AC16V7_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HIR6257662.1"
                     /translation="MSKVRADILLVNRGLAESRSQAQRLVMAGQVRADGQVIPKPSTP
                     VADDAVLVVEQGPPFVSRGGEKLHAALHAFPLDPASMVCADVGASTGGFTDCLLQHGA
                     AKVYAIDVGKGILHWDLRQNPRVIVMERCNARYLENLPEAVDLVTIDVSFISLQVLLP
                     VVKGWFRPPSSTQFQTGEAKPVGQVIALIKPQFEAGRREVSRGDGVIRDPSVHRRVLL
                     DVLSNAQEHGYYPRDLLRSPLLGPKGNAEFLAWFTYPASEQDNIEELINNLFL"
     gene            3170..>4032
                     /locus_tag="AC16V7_10860"
     CDS             3170..>4032
                     /locus_tag="AC16V7_10860"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR6257663.1"
                     /translation="MTNFRAVAIDYAHLNHQRFLDALKEFVSIPSVSTDPERSNDMQE
                     AAQWVADRLRDLDCLNVQLFPTAGYPVVYGELMKAGPAAKTILVYGHYDVQPAEPLEL
                     WESDAFEPEVRGDNLYGRGASDMKGQVMATLAAVEAVSHNGGMPVNLKFLIEGEEEIG
                     SPNLEEFLEAHKDMLSCDFAINPDTGMLAADLPTITYALRGLAYFELRVYGPSHDLHS
                     GLYGGVVHNPAQELCDQISGMHDSQGRVTLPGFYDNVRPIDQDERDELSRLPTTDAFY
                     REETGVPVLWG"
BASE COUNT          972 a         1019 c         1048 g          993 t
ORIGIN      
        1 taatgccacc ggtgctggtg tgccagcgcc agccttcatc gacctgttcg aattcagcct
       61 cagtatgtac agctcctgca atcactaagt cttccccatt cagtggcaat ggactgggga
      121 tgaaaaactg ctgttcgata ctgtcttttt gggctacgta ctgctcaaat aagccgccac
      181 gatcgtagga cacaaccccg gggtgttcgt taactggttg aaccacgccg gtatttacac
      241 caggcagcgc tgtttcgctt gtgcccacga agcccagttg ccatgcccac tgcggtccag
      301 cagggcgggg cgtaaattgc agaccatctg aattaaatgt ggctatatgg cgcgggtggc
      361 gcagtatata gccatcatcg gttgcctcta tatcattgac ggctaacgct atagcctggt
      421 caggattgat tggcgcattc gcgtgcaaca cgggagactg ttggagagtg attgaactgg
      481 gtgccagttc aggaacagaa aagaaaaatg gcattgctgg aaaattaaaa agaagagata
      541 acaataccag gttgcacaaa aatatttgac cctttttctg agccatatct gcctcctata
      601 ctgtatgaag ttcacatttc atgatagctt ggatattaat tgtgacaagc tgctctcgag
      661 caaatcgatt ttcagcccaa atgatggcta ttttgagcaa ctggagccaa tcgctggcgt
      721 gtcataaaaa aagataatcg ccaaggttta aagaacgtct ttaagaaacc cacatatttc
      781 cgatttttgc cattgcttaa tcggccaagg gcttctctgt tgatattaat tattaacgtc
      841 atcgggtggg ctatgttaca attttatact ttttctcaca atcgctgaaa ggacatcagc
      901 cagtatcctt attctgagat accgagtttg aaatgccagt caaaaccttc aacatccccc
      961 tcagcactcg tggtcatgcg gatatcctgg atatcaccga taaggtagca ggttgcgtgg
     1021 cagagagtgg tttgcaagac ggcattgtga cgatcttctg cccgtcatca accagcgccc
     1081 tgacaaccat tgagtatgag cgcggttgta tcagcgacct gcgccgcctg tttgatgaga
     1141 tcatcaatcc caaccgccat tacgatcacg atcaagcgtg gggggatggt aatgggcaca
     1201 gccatgtgcg tgctgccctg ctcaaggcct cactcacggt gccctttgtg cgcgctcgcc
     1261 ttaccctggg cacttggcag caaatcatct atgtcgattt cgacactcgt tccaggcgca
     1321 gggaactagt agtccaaatt attggggagg ttggctaacc tctaaggtca aacggtgagg
     1381 ttgatctcgc gccaccctat tttgtctgct ccgcgaaaac acccaagtgg ttacccaccg
     1441 gtcgttccca tcccggaatg ccgtgattga ttggcgagtt gagcaatgta gctttccccc
     1501 cctgctctat taccagggtg ctagagccgc cgccgtccag gttcatggct gtgtgcgcgc
     1561 catgggcaac caagatactt gccagttctt ctaaagttgc cccttcactg tagcctggtt
     1621 ggcgaccatc cacaaccaca ataatcaaag acctgccgcg cttatccaga ccgatggctg
     1681 tgcgcggttc cctagtaccc tccaggctgg gtaaaacttt gccatcctgc accaacatga
     1741 gattgccgga gatggcgttt atgatcttac ctggcggcgt accaaagcgg gcttgattgt
     1801 tgcgagtgaa atacagcacc ggctgctcat gtaagttatc agaatatact tttccgtcag
     1861 aggcagcaaa tccaatcggc tcaaccgcat cccccgagcg ggggtaatag tccaggatat
     1921 tattagagta ccagggtgta aatccatccc catttatggc gatttgaacg ccaaactgat
     1981 ccaggaattt tgatgtggtg cgggctttca gtgggcgctc agcctttgga tcaccggggg
     2041 tgactagcag gctaataccc ggggcgcgca aattgactgt gactacatga gcaaccatcg
     2101 ggcgggatga gttccgcact tcacgcgcat aggtaattcc ttgaaatagc gcctggttgg
     2161 ctggcttagg ctgagatcgg gggatgaaca taagccaatt aaagcccacc agcccgccca
     2221 gaatagtgaa tgtcatcaga atacaaagtg gaatgaagac cagtaaaagg cagcttcttt
     2281 tttgtttcat aaaaatagat tgttgattaa ctcctctata ttgtcctgct cgctggctgg
     2341 ataggtaaac caggccaaaa actcggcatt ccccttcggg cccaacagtg gcgagcgcag
     2401 caaatcgcgc ggatagtagc catgttcttg ggcgttagag agaacatcca gcagcacgcg
     2461 gcggtgcaca gacgggtcac ggattacgcc gtcaccgcgg gatacctccc tgcgaccggc
     2521 ctcaaattgc ggtttgatca gggcgatgac ctgaccaact ggttttgcct cacccgtctg
     2581 gaattgggtg gatgatggag gacgaaacca gcctttgacg acgggcaaga gcacttgcag
     2641 cgagatgaac gaaacgtcaa ttgtaaccaa atctacagct tcgggcaaat tttcaagata
     2701 gcgggcattg cagcgttcca ttacgatcac ccgagggttt tggcgcagat cccaatgcag
     2761 gatacccttg ccgacatcaa tcgcatagac ttttgctgcc ccgtgctgca gcaggcaatc
     2821 ggtaaaccca ccagtcgaag cgccgacatc agcgcagacc atgctggcag gatccagcgg
     2881 gaaggcatgc aaggcagcat gcaatttttc gccgcctcgc gatacgaaag gaggtccctg
     2941 ctctacaacc agcacagcat cgtcagcgac tggggttgag ggtttaggta tcacctgccc
     3001 atcagcccga acctgccctg ccattaccag gcgttgtgcc tggctccggc tctcggctaa
     3061 cccgcgattg actagcaata tgtccgcgcg tactttcgac atatcacaat tgtaactgaa
     3121 gatgccgggg tataattggg cgtgcatttt aattatctag gagacattca tgaccaattt
     3181 ccgtgcggtt gcgatagatt acgctcacct gaatcaccag cgttttcttg atgcgttgaa
     3241 agagtttgtt agcataccgt ctgtatctac cgatcctgaa cgaagcaatg acatgcaaga
     3301 agctgcccaa tgggtagccg atcgattgcg tgacttagat tgcctgaatg tgcaattgtt
     3361 tccaactgcc gggtatccgg tggtttacgg tgagttgatg aaggcggggc cagcggccaa
     3421 aaccatcctg gtctatggac attacgatgt ccaacccgcc gagccattag aattgtggga
     3481 aagcgatgca tttgagccag aggtgcgtgg tgataatctg tacggccgcg gcgccagcga
     3541 tatgaaaggt caggtcatgg cgactcttgc cgccgtcgaa gcagtgagtc ataatggggg
     3601 aatgcccgtg aatttgaaat ttctcatcga aggtgaggaa gagatcggct caccgaactt
     3661 agaggaattt ctcgaggcgc ataaagatat gctgtcttgt gattttgcca ttaatccgga
     3721 caccggcatg ctggcagctg accttccgac aattacgtat gcattgcgtg gcttggcata
     3781 ctttgagtta cgcgtatatg gccccagtca cgacctgcat tctggtctgt atggtggcgt
     3841 ggtgcataac cctgcccagg agttatgcga tcagatttct gggatgcatg actcgcaggg
     3901 gcgtgtaacc ctgccaggtt tttacgataa tgttcgtcct attgaccagg atgagcgaga
     3961 tgagctctcc cgcttaccta ctactgatgc tttttatcgt gaagagactg gcgtcccagt
     4021 tctttggggt ga
//
LOCUS       DBNHOH010000621         2945 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1704011, whole genome shotgun sequence.
ACCESSION   DBNHOH010000621 DBNHOH010000000
VERSION     DBNHOH010000621.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1704011"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="AC16V7_10865"
     CDS             complement(<1..220)
                     /locus_tag="AC16V7_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HIR6257664.1"
                     /translation="MLSTSSQIRSLGFTTRQLHAGQQPDLSTGARAVPIYQTTSYVFN
                     DTDHAARLFALEEVGNIYTRIMNPTNDVF"
     gene            complement(643..978)
                     /locus_tag="AC16V7_10870"
     CDS             complement(643..978)
                     /locus_tag="AC16V7_10870"
                     /inference="COORDINATES: protein motif:HMM:NF014132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HIR6257665.1"
                     /translation="MAEYTVEQLVRNHEKAFKPEAAEGVDAIIQYHLTGEEGGDWIIN
                     IHEGKCKVAQGVAENPKMTLTAEAQDFKDVLTGKANGMQYFMQGRLMLAGDLNLAMKL
                     TTFFKMSSN"
     gene            complement(1080..2285)
                     /locus_tag="AC16V7_10875"
     CDS             complement(1080..2285)
                     /locus_tag="AC16V7_10875"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HIR6257666.1"
                     /translation="MMINRSDPNREAVIVAAVRTAVGRAKKGSLATVRPDEMAALVIR
                     DLLERAPALDPAQIDDLILGCAFPEGEQGLNLSRLVGFRAGLPDSVPAETINRFCSSG
                     LQAIAHAAFAIMAGQMEVAIAGGTESMSMVPMTGFKFSPSPYLAENAPQAYTNMGLTA
                     ENVAAKYHISREDQDLFALRSHQRAVAAVEAGLFDAEIIPVEVDIVESNGNGKPKRRS
                     FTFSREEGPRSDTSLEALAKLRPVFKEGGTVTAGNASQMSDAAAGVIIMSRAKAEALG
                     LTPLVRFVAFAVGGVPPEIMGIGPVVAVPKALKLAGLSLKDIGLIELNEAFAAQAVTV
                     IRELGLDEEITNVNGGAIALGHPLGATGAKLTTQIISEMKRRDVQFGMVTMCIGGGMG
                     AAGIFENLN"
     gene            complement(2363..>2945)
                     /locus_tag="AC16V7_10880"
     CDS             complement(2363..>2945)
                     /locus_tag="AC16V7_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HIR6257667.1"
                     /translation="ELYIGMVEAGAGVIPAWGGTKEMLRRILNPPMLTKNTEALPFLQ
                     RIFEQIGMAKVATSAEEARQMGILGPADRVILNREHLLAEAKREVLHMLDDGYRPPLP
                     EKIYAAGRDALAAMRVGIYMLMKGGYITEYESHIANKLAYVMTGGELSSPTWVDEQYI
                     LDLEREAFLSLAGEKKTQERMWNLLQTGKPLRN"
BASE COUNT          671 a          832 c          731 g          711 t
ORIGIN      
        1 caaacacatc gttcgtgggg ttcatgatgc gggtatagat gttccccacc tcctccaggg
       61 cgaagagacg ggcggcatgg tcggtgtcgt tgaaaacata cgaggtggtc tgatagatcg
      121 gcacagctct cgctcctgtg gagagatcgg gctgttgtcc agcgtgtagc tggcgggtgg
      181 taaagccgag cgaacggatt tgactactgg tggatagcat cggttgtttc tcctttttta
      241 ttttcggtat aaaaaaagcg gcggacagct tggcgcaatc tctaaaagag ttttgcgaga
      301 agctgtccgc cgccttggca aacctggctt aagcgtcaat caggccaatt gaccctcacg
      361 ctccttcgag gcggtggttg cagctcccgc ctcgtgcaca tcaacatcga cataaatagc
      421 aacataaaaa acttcctgtt atcctgtgct agaaattgtg ccaattctaa gaaataaatc
      481 gtattttgtc aatgatcagg aacaaattca cgcactggct ggaaaggtac ctcgccagtg
      541 cgtgaatact ctcatctata accccaggtg ccttgctgtc ttcaggtcac tggggattaa
      601 caatcggtca aaagcgttag aaagctctgg ctcttgtgtt ggctaattcg atgacatttt
      661 gaagaaggta gtcagcttca tcgccagatt caagtcgccc gccagcatca gacgaccttg
      721 catgaagtac tgcatgccat tagccttgcc ggtgagcaca tccttgaaat cctgtgcttc
      781 agcagtgagg gtcatcttgg gattttcagc cacaccttgg gcgaccttgc atttaccctc
      841 atggatattg atgatccaat cgccaccttc ttcaccggta agatggtact ggatgatagc
      901 atccacccct tcagcagctt ctggtttgaa ggctttctcg tggttgcgga ccagttgttc
      961 aacagtatat tcagccataa ataatcgctc ctatagaaaa agagaatgtt gggttaaagc
     1021 ttgtaattca tgataagcca tctgtcaata tcggcttcta tgcgtaagcc ggactgattc
     1081 taattgagat tctcaaatat cccagccgcg cccatgccgc caccgataca catagtcacc
     1141 ataccgaact gcacgtcgcg acgcttcatt tcagagatga tttgcgtggt tagcttggcg
     1201 cctgtggcgc ccagtggatg ccccaaagcg atggcgccgc cattgacatt agtaatctcc
     1261 tcgtccaatc cgagctcgcg aatcacggtc actgcttggg cagcaaaagc ctcgttcaat
     1321 tcaatgagac cgatatcctt gagagataaa cccgccagct tgagcgcctt cggcacagcc
     1381 acgaccgggc caatacccat gatctccggc ggcacacctc ccacagcaaa agcgacgaag
     1441 cgcaccaatg gcgtaagtcc taaggcttcg gctttggcac gcgacataat gatcacccca
     1501 gcagcggcat cagacatttg cgacgcattc cccgccgtga cagtcccgcc ttccttgaac
     1561 acaggtctga gcttcgcaag ggcttccagg ctggtatcgc tgcgtggtcc ttcctcccga
     1621 gagaaggtaa acgatctacg cttgggcttg ccgtttccgt ttgattcgac gatatccacc
     1681 tcaacaggga taatttccgc gtcaaatagc ccagcttcta ctgctgccac agcgcgttgg
     1741 tggcttcgca aagcaaagag atcttgatcc tccctgctga tgtgatattt cgcagcaaca
     1801 ttttccgcgg tcaatcccat attggtgtaa gcttggggag cgttctccgc caaataaggg
     1861 gatggcgaga atttgaaacc ggtcattggc accatgctca tcgattctgt acccccggcg
     1921 atggcgacct ccatctgccc agccatgatg gcgaacgccg cgtgggcgat ggcttgcagt
     1981 ccagacgagc aaaaacggtt gatcgtctca gctggcactg aatctggcaa tccagcccga
     2041 aatcccacca agcgcgagag gttcaaaccc tgctcccctt ctggaaaggc acaaccgagg
     2101 atcaagtcgt caatttgggc cggatccagc gcaggggcgc gctccaggag atcacggatc
     2161 accagcgcag ccatctcatc tgggcgaacg gtcgcaaggc tgcctttttt cgccctgcca
     2221 acggcggtgc gcaccgcggc gacgatcaca gcctcgcggt ttgggtcact tcggttaatc
     2281 atcatgttct ctcctaattg cacacggata aacatagcca ctcaatggag ctgatccgtg
     2341 tatattcttc atatcgatcc acctaatttc ttagaggctt tcccgtttgc aacaagttcc
     2401 acatgcgttc ttgggtcttc ttctctcctg ccagcgagag aaatgcctct cgctccaggt
     2461 ccagaatata ttgctcatcg acccaagttg ggctgctcag ttcgccgcca gtcatcacgt
     2521 atgccagctt gttggcaatg tgcgactcat actcggtgat gtaccccccc ttcatcagca
     2581 tatagattcc tacccgcatg gctgccaggg catcccgccc agcggcgtag attttctcgg
     2641 gcaagggcgg gcgataaccg tcatcaagca tatgcagcac ttcacgcttg gcttccgcca
     2701 gtagatgttc gcggttgagg atcactcgat ccgccgggcc aagaataccc atttgtcgtg
     2761 cttcttcagc actagtagca acttttgcca tgccgatctg ctcgaagatc cgctgtaaga
     2821 aaggcaatgc ctcggtattt ttggtgagca taggcgggtt gaggatgcgc cgcagcatct
     2881 ccttggtgcc gccccaggca gggattacac ccgccccggc ctccaccata ccgatataca
     2941 gctcg
//
LOCUS       DBNHOH010000622         7929 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1575179, whole genome shotgun sequence.
ACCESSION   DBNHOH010000622 DBNHOH010000000
VERSION     DBNHOH010000622.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7929)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7929)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7929
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1575179"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1384)
                     /gene="yqeC"
                     /locus_tag="AC16V7_10885"
     CDS             complement(<1..1384)
                     /gene="yqeC"
                     /locus_tag="AC16V7_10885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HIR6257668.1"
                     /translation="MLINEALRLGDAPRLALVGAGGKTTALFRLGRELLARAPHPDTY
                     TVIATATTHLGIEQARWADHHYIVRQPVDVDAYAAELPAGIVLFTGSESGEERLEGVS
                     SGVLERIRVLADAHNIPLLIEADGSRMKPVKAPAEHEPVIPPFVDTVVVLAGLSALGK
                     PLATEWVHRAEIFAGLAGLPQGSPITPEALTRVLNHPQGGLKSIPEGARRLAFLNQAD
                     TPELQSVAGAMAKQLLSSFEAVVIAALYPSGPQQPSSPSPSTLPVVSSPVIAVYEPIA
                     GVILAAGESRRYGQPKALLPWRGEPFIRHVIRAARRAGLSPVIVVGGAHTPQIRLACA
                     DLDAQLVHNPEWEEGQSASLRVGLSALTAQVGAAVFLLADQPQVPNGLVTALVEAHAA
                     GLPTIVAPLVDGQRANPVLFDRRAFTELMTLRGEMGGRALFSKYPITWIPWHDPRILL
                     DVDTPEDYQAL"
     gene            complement(1459..2271)
                     /gene="yqeB"
                     /locus_tag="AC16V7_10890"
     CDS             complement(1459..2271)
                     /gene="yqeB"
                     /locus_tag="AC16V7_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012808340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HIR6257669.1"
                     /translation="MIALIRGCGDLASGVAIRLHRAGVRVVMTELQQPLAVRRLVCFS
                     EAVYTGECAVEGVTGRRVNNPTDLLRILQVLSKGHIAVLIDPDGISIKHLHPHIVIDA
                     RMIKKNVPLIETSIKLIIGLGPGFSAGENCHAVIETNRGHMLGRVIWDGPAEPDTGEP
                     DVVANRRSERVLRAPTDGELQAHAEICDHLEAEQLVAEVAGQPLYAPFKGVLRGLLRS
                     GVQVTRDMKVGDVDPRDDPRYCTLVSDKSLAIAGGVMEAILSRTELRKQLWD"
     gene            complement(2275..5010)
                     /gene="ppc"
                     /locus_tag="AC16V7_10895"
     CDS             complement(2275..5010)
                     /gene="ppc"
                     /locus_tag="AC16V7_10895"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF000584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HIR6257670.1"
                     /translation="MEIAATIRLLGELLGQVITTEESPGIFDLEERVRGYAKARRSGL
                     AGSPQRVQAANRLAREIRALTSQSARPVAGAFKLYFDLVNLAEENFRANLLRQEEFAN
                     HPHPIHDSIGEAINVLKDSGVSHDEMGQLLDDLHIELVLTAHPTEAKRRTILSKLQRI
                     AMQIRRLERGDLPPRQVVSSRKAILEEITALWHTDPARTTRIKVTDEIRTTLYFVDEV
                     FWDVLPRIYADLDAALAQHYPGLKATHPWLSLASWVGGDRDGNPNVTHAVTAEALRLH
                     RGLAVERHRLTLQDTARRLSLSASRVPPTPELAAWFESRRPLPAHVSFLESRYSNEPY
                     RLAISLLADDLAEASKDDVTACLLSRNPTNNRLRSNDFIYPLKIIRQSIPELVADGLP
                     LTVQRHFEIFGLHAARLDIREDSSRLSEALGELLRALGIHAHFEHANQAERLVLLTEL
                     LASPKPDFAPQPGVTPRTAETFALFQLVNRTREVYGAELIGPVVISMARGAADVLAVL
                     LLARWAGCANCLDIVPLFETLDDLRAAPKVMSELFNLDVYRAHLATCRDHQMVMVGYS
                     DSNKDGGYLAANWALYRAQEDIAQVCKQHGVRFTLFHGRGGTVARGGGPANRAIRAQP
                     PATVQGRFRLTEQGEVISSRYAEPQLAYRHLEQIINAVLLSSFPDKMDHGRAVQEKWR
                     VAMNGMAMAAHRAYRRLVYETPGFIDYWLVVTPIEEIQRLQIGSRPAVRQSEENSLAR
                     VRAIPWVFSWMQSRFNLPGWYGLGSGLAASPLALPEMKEMYAEWPFFRSLFDNAEMSL
                     LKADLDIAALYSALMPDQVFAKRIFDDIQREYDRTQELVLAISGHAQLMDGDPSIQRS
                     IHLRNPYIDPLNYIQVEMLQRLRALVNPDCEEAEQLRQVVVLTINGIAAGLRNTG"
     gene            complement(5060..7255)
                     /locus_tag="AC16V7_10900"
     CDS             complement(5060..7255)
                     /locus_tag="AC16V7_10900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR6257671.1"
                     /translation="MTSIGKSVHRIDAFGKVTGQTLFPGDINLPDQASMKILFANLPH
                     AIIRRIDTSQAEALPGVIAVFTAKDVPVNEYGLIMNDQPVLCGPGSSKPFADRVRFVG
                     DQVALVVAETEAIAARGRDLIQVEYEDLPVVTHALEAMRDGAPLLHPDRDSNIFCHYR
                     IRKGDAESAFSQADVIVEGEYHTPAQEHAYLQPEAGLGYIDEDGRLTVQVAGQWTHED
                     QKQIAHALGLPQDQVRVVYPAIGGAFGGREDMSVQIVLALASWRLSQRGINRPVKIIW
                     SREESIIAHHKRHPYFIKTRWGATRQGKLVAAEVEVIADGGAYAYTSTKVLGNATLMC
                     TGPYEIPNVKVDSYAVYTNNLPNGAFRGFGGPQGAFAAEMQMNHLAEKLGMDPVEIRQ
                     RNVLREGALLSVGTPLPKGVSMPQVVERCGLESARLKEAISTSKVQPSHLKRGFGFAC
                     AFKNVGFSFGFPEQCWATIELHGGAKIERAVVHHAGADVGQGAHTVFAQMAAEALDLP
                     MDKVDLVVSDTAYTDNSGSSSASRMTFMAGNAIKGAAELALQKWRAEERPAIATYQYR
                     PPSTTPYDPQTGKSEPNISYGYVAQAVTVEVDTETGHVRILDVVSVDDVGKALNPLQV
                     QGQIEGAVVQAAGYTILENFVQSGGYVQTQHLSTYLIPTVLDVPERVQSLILEYPDPR
                     GPWGARGMAEMPFLPLAPAIVAAVHDATGVWFHEFPLIPERVLRGLGKI"
     gene            complement(7483..7770)
                     /locus_tag="AC16V7_10905"
     CDS             complement(7483..7770)
                     /locus_tag="AC16V7_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA recognition motif domain-containing protein"
                     /protein_id="HIR6257672.1"
                     /translation="MDTKLYVGNLSYDTTEDTLRSLLSQAGTVESVDMITDRDTGRPK
                     GFAFVTMGSQVDAEKAITMFNEKIVDNRTIKVNIARPREERPRAGGYGYRR"
BASE COUNT         1815 a         2257 c         2163 g         1694 t
ORIGIN      
        1 acaaagcctg gtaatcctcg ggcgtgtcca cgtccagcaa tatccgaggg tcatgccagg
       61 gtatccaggt aatcgggtac ttcgagaaaa gcgccctacc gcccatctcg cctcgcagtg
      121 tcatgagctc cgtaaaggca cgccggtcaa agagcaccgg gttggctcgc tgcccatcca
      181 ccaatggagc gacgatcgta ggtaagcccg ccgcatgagc ctcaaccagc gcagtgacca
      241 gtccattggg cacctggggc tggtcggcca aaaggaatac cgccgccccc acctgagccg
      301 tcaacgcgct caaccccacc cgcagtgaag cgctttgccc ctcttcccac tccgggttgt
      361 ggacaagttg ggcatccaaa tcggcgcagg ccagacgaat ttgaggcgtg tgggcaccac
      421 cgacaacgat cacaggagac agtcctgccc tgcgcgcggc gcggatgaca tggcgaatga
      481 acggctcccc tctccagggt aaaagtgcct tgggctgacc atagcggcgt gattcacccg
      541 ccgccaggat cacgccggcg attggctcat agacggcgat gaccggtgag gatacaacgg
      601 gtaaggtaga ggggctaggg gaggagggtt gctggggacc ggaaggatac agagcagcga
      661 tgacaacagc ctcgaaagat gacaataatt gtttcgccat cgcccccgct acggactgca
      721 attctggcgt gtcggcttgg ttaaggaatg ccaagcggcg cgccccttcc gggatacttt
      781 ttaagccccc ctgcgggtgg ttcaagacac gggtcagcgc ctctggagtg atgggtgaac
      841 cctgcgggag tcccgcgagt ccagcaaaga tctccgcacg gtgcacccac tccgttgcca
      901 gcggcttgcc cagtgcggat aaacctgcca gcaccacgac cgtatccaca aagggtggga
      961 tgaccggttc gtgttctgca ggggctttga ccggtttcat acgagagcca tccgcttcaa
     1021 ttagcaatgg gatattatga gcatccgcta aaacgcgaat tctctccagg acacccgagg
     1081 aaacgccctc caaccgttcc tcgccgctct cgctgccggt gaacaagacg atcccagcgg
     1141 ggagttctgc tgcataggca tccacatcta ccggttgccg cacgatgtag tggtggtctg
     1201 cccagcgagc ctgctcaatc cccagatggg ttgtggctgt ggcaataaca gtgtaggtat
     1261 ctgggtgcgg tgccctggcg agcaactcgc gccccaatcg gaagagtgcc gtggtcttcc
     1321 ccccagcacc taccaacgcc agtcggggag catcgccaag gcggagagct tcattgatta
     1381 acatggaaat tgtgtgtcat ccatcggtgc tggcttgata tgcgttaagt gggagtaata
     1441 gagacaggag aaaaaatact agtcccacag ttgtttgcgc agttccgtgc gagacaggat
     1501 agcttccata accccaccgg caatcgccag cgatttatca gaaaccagcg tgcaatagcg
     1561 ggggtcatca cgggggtcta cgtcgccaac cttcatgtct cgcgtcactt ggacaccaga
     1621 gcgcagcagt ccgcgcaata ctcctttgaa cggcgcgtat aggggttgcc cggccacctc
     1681 ggcgacaagc tgctctgcct ccaggtgatc gcaaatttct gcgtgagcct gcaactcccc
     1741 gtcggtcggg gcgcgcaaga cacgctctga gcggcggtta gccactacat ccggctcacc
     1801 ggtatctggc tccgctgggc catcccagat cacgcgaccc agcatatgac cgcgattggt
     1861 ctcaataact gcatggcagt tctccccggc tgagaaccct ggccccaacc caatgatcag
     1921 cttgatcgaa gtctctatca aagggacgtt tttcttgatc atgcgcgcat cgatgactat
     1981 atgcgggtgt aaatgcttga tcgaaatgcc gtctgggtct attaaaacgg caatatgacc
     2041 cttggaaagg acttgcagaa tgcgaagcag gtcggtggga ttgttcactc gccgtccagt
     2101 cacaccttcc acagcacatt cgccggtgta tactgcctcg ctaaagcaga ccagcctgcg
     2161 gaccgccagg ggctgctgga gttctgtcat caccacgcgc acaccagccc ggtgcagccg
     2221 tatagccaca ccgctggcta aatctccaca accacggatc aaagcaatca tgttctaacc
     2281 tgtatttctt aatccagcag cgatgccatt gatcgtcagc acgaccactt gccttagctg
     2341 ctcagcttct tcacaatcag gattaaccag ggcacgtagc cgctggagca tttccacctg
     2401 aatgtaattt aatgggtcaa tataagggtt gcgcaaatgg atggagcgct ggatgcttgg
     2461 atcgccatcc attaattgtg catgcccgct gattgccagc acaagctctt gtgtgcgatc
     2521 atattccctc tggatatcat cgaaaatcct cttagcaaag acttgatccg gcattaaagc
     2581 cgagtaaagg gcagcgatgt cgaggtccgc ttttaacaag gacatttcag cgttgtcgaa
     2641 caggctgcga aagaagggcc actccgcata catctctttc atctctggaa gcgccagggg
     2701 tgaagcagcc aacccggatc ccagcccgta ccagcccggc agattgaaac gactctgcat
     2761 ccaggagaac acccagggaa tggcacggac gcgcgcaaga gaattttcct cagattgacg
     2821 gacagccggc cgggagccta tctgaaggcg ctggatctcc tcgatagggg tcacaacaag
     2881 ccagtagtct ataaaaccgg gcgtctcata aaccaatcgg cggtaagcac ggtgcgccgc
     2941 catagccatg ccattcattg cgacccgcca tttttcttgc acagcacgac catgatccat
     3001 cttatctgga aatgaagata gcagcaccgc gttgataatc tgctccaagt gacgatatgc
     3061 caactgtggt tcagcgtagc gagaggaaat cacctcgcct tgttctgtca gacgaaagcg
     3121 cccttgcact gtagcgggtg gttgggcgcg tattgcccgg ttagcaggcc cacctccccg
     3181 tgcaacggtc ccaccacgac cgtgaaacag ggtaaaacgc accccatgct gtttgcaaac
     3241 ctgagcgatg tcttcctgcg cccgatacaa ggcccaattc gctgccagat aaccgccatc
     3301 cttattgcta tcggaataac ctaccataac catttgatgg tcacggcagg tcgccagatg
     3361 ggcccggtat acatctaaat tgaacagctc tgacatgact ttgggcgcgg cacgcagatc
     3421 gtccagggtt tcaaacaatg ggacaatatc taggcaattt gcgcaacccg cccagcgcgc
     3481 cagaagcagt accgcgagaa catctgcagc accacgggcc atcgaaatga caaccggtcc
     3541 gatgagctca gccccatata cttcacgggt gcggttcacc aattgaaaca gggcaaaggt
     3601 ttccgctgtt cgaggggtca caccaggttg aggggcgaag tcaggtttgg gagatgccag
     3661 caattccgta agtaaaacca ggcgctcagc ctgattagca tgctcgaaat gagcatgaat
     3721 ccccagcgcg cgcaatagtt cgcctagcgc ttcgctcaaa cgggaagagt cttcgcggat
     3781 atccaaacgt gctgcgtgca agccaaagat ttcgaagtgc cgttgaactg tgagcggcaa
     3841 accatcggcg accagctctg gaatagattg gcgaataatt ttcaaaggat atataaaatc
     3901 atttgagcga aggcggttgt ttgtcgggtt gcggctcaag aggcaagcag tcacatcatc
     3961 cttggaggct tctgccagat catcggcaag caaggaaatc gctaggcgat agggctcatt
     4021 cgaataacgg ctctctaaaa aggacacatg cgccggcaac ggtctgcggc tctcaaacca
     4081 ggcagccagc tctggtgttg gcggcacccg gctggcgctc aaactcaaac gccgcgccgt
     4141 atcttgcaat gtaagccgat gccgctctac tgccagccca cggtgcaatc ttagcgcctc
     4201 agccgtaaca gcatgggtca cattcgggtt gccgtcacgg tcacctccca cccaggaagc
     4261 caggctcagc caagggtgcg tggcttttaa accaggatag tgctgcgcaa gtgcagcgtc
     4321 taagtccgca tatatgcgcg gtaacacgtc ccaaaatact tcatctacga agtatagggt
     4381 tgtacgtatc tcatcggtga ctttgatccg agttgtgcgg gcggggtctg tatgccagag
     4441 tgcggtaatc tcctccaata tggctttgcg ggatgaaact acttggcgcg gcggcaggtc
     4501 accgcgctcc aggcggcgta tctgcatggc aatcctttgc aacttggaaa gaatggtgcg
     4561 acgtttcgct tcggtaggat gagctgtgag gaccagctcg atgtgcagat catctaaaag
     4621 ttgtcccatc tcatcgtgag aaaccccaga atccttcaat acgttgattg cttcgccgat
     4681 cgaatcgtga atggggtgcg ggtgattggc gaactcttcc tggcgcagca ggttggctcg
     4741 aaaattttct tcagcgagat tgaccaggtc aaagtacagc ttgaaggcgc ctgccacggg
     4801 acgagcagac tggctggtca gggcacgaat ttctcttgcc aacctattgg cggcttggac
     4861 gcgttgcgga ctgccagcga gtccacttcg ccgggctttg gcatatccac ggacgcgctc
     4921 ttctaggtcg aagatccccg gggattcttc tgtagtgata acctgaccga gaagttcacc
     4981 aagtaaacga attgttgccg ctatttccat gccggaccag tggggagtac ttgcaatgac
     5041 cgttgcaatc cagtcaatgt cagattttgc ctaaaccgcg caaaacgcgc tcggggatga
     5101 gcgggaattc gtggaaccac acaccggtcg cgtcgtgaac cgcggcaacg atcgcaggcg
     5161 ccagcggcag gaaaggcatc tctgccatac cgcgcgcgcc ccaaggtccg cgcgggtctg
     5221 ggtattccag aatcaaagat tgcacccgtt cgggcacatc cagtactgtg ggaatcaaat
     5281 aggtagagag atgctgggtc tgtacgtagc ctccgctttg aacaaagttc tccaggatgg
     5341 tgtatccggc agcctgtacg accgccccct cgatctgtcc ctgcacctgc aggggattca
     5401 acgctttgcc cacatcatca accgaaacca catctaggat gcgcacatgc ccagtctcgg
     5461 tatccacctc cacagttacc gcctgggcta cgtatccgta gctgatattt ggctccgact
     5521 tgccagtctg gggatcatag ggcgtcgtgc ttggagggcg gtactggtaa gtcgcgattg
     5581 ccggacgttc ctcagcgcgc catttctgca aggccaactc ggcagcgccc ttaatcgcat
     5641 tgcctgccat gaaagtcatg cgcgaggccg acgaactgcc tgagttatct gtataagccg
     5701 tgtcggaaac gaccagatcg accttgtcca tcggcaggtc aagggcttcc gccgccatct
     5761 gtgcgaagac ggtgtgcgca ccctgcccta catcagcccc cgcatggtgg acaacagcgc
     5821 gctcgatctt tgcgccgccg tgaagctcga tggtcgccca gcactgctct ggaaagccga
     5881 aggagaagcc cacattctta aaggcgcagg caaaaccgaa accgcgcttc aggtgagatg
     5941 gctgtacctt ggatgtgctt atcgcctcct ttaacctggc gctctctaac ccgcaacgtt
     6001 cgacaacctg cggcatgctg acccctttgg gcaaaggtgt gcccacagag agcagcgcac
     6061 cctcgcgcaa aacgttgcgt tggcggatct ccaccgggtc catgcctagc ttttcggcca
     6121 ggtggttcat ctgcatctca gcggcaaacg cgccctgggg tcccccgaag ccgcgaaacg
     6181 caccgttagg caagttgttg gtgtagaccg catacgaatc cactttaaca ttggggatct
     6241 cgtacggtcc agtacacatc aaggtggcat tgcccagcac cttggtggaa gtgtaggcgt
     6301 aggcaccacc atctgcaatg acttccactt cggctgcgac tagtttaccc tggcgagtgg
     6361 caccccaacg ggtttttatg aagtaaggat ggcgcttatg gtgcgcaata atcgattctt
     6421 cgcgactcca gatgatcttt actggacggt tgatgccgcg ctggcttaac cgccaggatg
     6481 ctagtgccag gacaatctgc accgacatat cttcgcgccc gccaaatgca ccgcctatcg
     6541 ccggatatac cacgcgcacc tgatcttgcg gtagtccaag agcgtgggcg atctgcttct
     6601 ggtcttcatg cgtccactgt cctgcgacct ggacagtgag ccgcccgtct tcgtcaatgt
     6661 agcccagccc agcctcgggt tgcaggtaag catgttcctg ggcaggtgtg tgatactcgc
     6721 cttcaacaat cacatcggct tgtgagaagg cagactccgc atctcccttg cggatgcggt
     6781 aatggcagaa gatattggag tctcggtcgg ggtgcaatag aggtgcgcca tctcgcatgg
     6841 cttccagcgc atgggtgaca acaggtagat cctcatactc cacttggatc aggtcgcgcc
     6901 cccgggcagc aatggcttcg gtctcggcaa ccaccagtgc tacctggtca cccacgaaac
     6961 gcacacggtc agcaaaaggt ttgctggaac caggaccgca cagcactggc tggtcattca
     7021 tgatcagccc gtactcgtta acaggtacat ccttggcggt gaagacagcg attacgcccg
     7081 gcagggcttc ggcctggctg gtatcgatac ggcggatgat ggcgtgagga aggttggcga
     7141 acagaatctt catacttgcc tgatctggca agttgatatc accgggaaag agtgtctgcc
     7201 cagtgacttt gccgaacgca tcgatgcggt ggacggattt tccaatgctt gtcatgggta
     7261 ctccgatgag atggttggga taacaaacgt tatatctcta ggtgagatgt gtgtattata
     7321 ccccgcgagc gatcagtcat agtacgccga gcgagttgag aagtcctgtg tgggggtaca
     7381 gtggatcagg atagatcccc agtccatttg ttcatctgtg gaagtactta aaataaaaaa
     7441 ccccaccgta attgggtggg gtccctatga catgtggaat gattaccggc ggtagccgta
     7501 accacccgcg cgcggccgct cttcgcgggg acgggcgata ttgaccttga tcgtgcgatt
     7561 atcgacgatc ttctcgttga acatggtaat cgctttctct gcatcaacct ggctgcccat
     7621 ggtgacaaag gcaaaacctt tcggtctccc agtatcccga tctgtgatca tatccacgga
     7681 ttccactgtg cccgcctggc tcagcaggct gcgcaaggta tcttcggtcg tatcataaga
     7741 caaatttcca acgtacaatt tcgtatccat ttctaacttt ctccttaaat ttaattgtgc
     7801 agatccgtgg ttaacaaaca ccgccattcc cttataaagc ggaatggcga cacatcaata
     7861 cacgcaaatc tgattataag actcaagtat tctaccacat aaagcactca agacttgtaa
     7921 atggggata
//
LOCUS       DBNHOH010000623         3386 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1255180, whole genome shotgun sequence.
ACCESSION   DBNHOH010000623 DBNHOH010000000
VERSION     DBNHOH010000623.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1255180"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1478)
                     /locus_tag="AC16V7_10910"
     CDS             complement(93..1478)
                     /locus_tag="AC16V7_10910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6257673.1"
                     /translation="MSSATADVLICGAGICGVSAAYHLTVKQGFKRVILVDERAPMSF
                     TSDKSTECYRNWWPGPGDTMVRFMNRSIDLLEELAQDSGNYFGLNRRGYVFLTADPQK
                     AAQYERAAREISQLGAGPLRIHRGLPADPSYIPSPDDGFQNLPTGADLILDPELIRQT
                     FPFVSERAVAMLHIRRCGWMSAQQLGAYMLDQAKARNLEVLQGKVTQVKVEGGRVRSV
                     TVETSLGSQSVATDHLVIAAGPYVKQVSSLVGVNLPVYNELHAKIAIQDYLGIVPPHV
                     PLMLWDDPIQLRWSAEEQRELAESHETRWLLETFPSGVHFRPEGKGQSAFLLGLWTYD
                     IQEQEPLPNPSYDPYYPEVILRGLTHMIPGLGVYIGRMRKPRVDGGYYCKTRENRPLV
                     GPLPVEGAYIFGAVSGFGIMAGMAGGELLAAHVAGLTLPDYANDFLLSRYENPQYQRL
                     LAQWETTSGQL"
     gene            complement(1624..2802)
                     /locus_tag="AC16V7_10915"
     CDS             complement(1624..2802)
                     /locus_tag="AC16V7_10915"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR6257674.1"
                     /translation="MKQQGYRRSIYVLLVGLLLPVLLFSCRFFTPENVQTIPDQPPTV
                     VVSTPVPPERTTTIEITGEDEVLVDLYTRVNPSVVNVTVYQRQSGDLLPVGQGSGFVF
                     DTNGNIVTNAHVVHAAEQVEVTFSSGVVRRAELVGEDLNSDLAVVRAENLPEDAVPLP
                     LGDMDSLAVGQSVVAIGNPFGLDGTLTRGIISALGRTIPALTPFSIPQSIQTDAAINP
                     GNSGGPLLNLAGEVIGVNAQIETGGTTRSNLGIGFAIPVSIVRLVVPDLIQDGVHHWA
                     WLGIRGGSLTPPLVEAMDITVDKGAYIAEVISRGPSSKAGLRGSNNTVRVNGRDIDVG
                     GDVITAIDGQPVNTFDDLLIYIALKSPDQEVNLTILRDGETLEIPVTLEERPESLEQP
                     "
     gene            complement(2933..>3386)
                     /locus_tag="AC16V7_10920"
     CDS             complement(2933..>3386)
                     /locus_tag="AC16V7_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257675.1"
                     /translation="TETLPEPPTPTASSPPTIADPTATPIVPTPTLTASAIPMTPTSS
                     PTPSDHILWSDASEYLGEERTVCGPVAGTHYAKESRGKPTFLNIGEDYPSPDRFVVLI
                     WVEYRDNFSNKPEDYYDGKTICAQGEILEYEGIFEIEVQSPSDIEVQQ"
BASE COUNT          818 a          926 c          870 g          772 t
ORIGIN      
        1 ccagttgttg aattcattgc cctaaagttt ttcgctaacc ctttggattg cgcaaatcta
       61 tccccctgaa cataagaaac agccatgctc tttcagagct gaccggaagt ggtctcccac
      121 tgcgccagaa gtcgttgata ttgggggttt tcgtagcgac tgagcaggaa gtcattggcg
      181 taatctggca gggttaaacc agccacatgg gcagccagca actcaccgcc agccatgccc
      241 gccatgatac cgaagccgga taccgcacca aaaatatagg caccctctac cggtaaaggg
      301 cccaccaagg ggcggttttc ccgcgtcttg caataataac caccatccac ccttggtttt
      361 ctcatccgcc cgatataaac acccagtccc gggatcatat gagtcaggcc gcgcaggatt
      421 acctccgggt agtatgggtc gtagctaggg ttaggcagcg gttcttgttc ctggatgtca
      481 tatgtccaca accccaacaa aaacgcgctc tggccttttc cctccggacg gaaatgcaca
      541 ccggacggga aagtttccag caaccagcga gtctcatgac tttcagccaa ctccctttgt
      601 tcctctgcac tccaacggag ctggattgga tcatcccaca gcatcaaggg gacgtgcgga
      661 ggaacaattc ccagatagtc ttggatggca atcttggcat gcaactcgtt ataaactggc
      721 agattcacgc caaccaatga agacacctgt ttcacataag ggccagctgc aatcaccaga
      781 tggtctgtcg ccacgctttg ggaaccgagc gaggtttcaa cggtgaccga gcgcacgcgc
      841 ccgccttcta cctttacttg cgtgaccttt ccctgaagaa cttcaagatt cctggctttt
      901 gcttgatcca gcatgtaagc tcccaattgc tgggcgctca tccatccgca gcgccgtata
      961 tgcaacatgg ctaccgcccg ctcgctcaca aaagggaagg tttgacggat caactctgga
     1021 tcaagaatta agtctgctcc agtgggaagg ttttggaagc catcatcagg cgagggtatg
     1081 tagcttggat cagcgggcag gccgcgatgg attcgcaaag ggccggctcc tagctgagag
     1141 atctcgcgtg cggcgcgctc atattgagcc gctttctgcg ggtctgcggt aaggaagaca
     1201 taccccctgc ggttcaaacc gaagtaatta ccactatcct gcgccagttc ttccagcaga
     1261 tcgatactgc ggttcataaa ccgcaccatg gtgtcgcccg ggccaggcca ccaattgcgg
     1321 tagcactcgg tggacttgtc gctggtgaag ctcatgggag cacgctcatc caccaggatg
     1381 accctcttaa agccctgttt gacagtaaga tgatatgctg ccgagacacc acaaatcccg
     1441 gcaccacaga tcaacacatc ggctgtagca gaagacatga ggctattcag atacccttgg
     1501 cgattatctg tcgtcacccc cctcaagtgg gaggtgacga caatggaagt aataggaatt
     1561 acgagctaaa caagaagggt ggtagcaaaa caccctgccc aacacctttt gaggtgagga
     1621 ctactatggt tgttccaggc tctcagggcg ttcctctaaa gtaaccggaa tctccagggt
     1681 ctctccatcg cgcaggatgg tcaaattgac ttcttgatct gggctcttca gggcaatata
     1741 gatcaacaga tcgtcaaaag tattcaccgg ttggccatcg atagcggtaa tcacatcgcc
     1801 gccaacatcg atatcacgcc cattaactct cacagtattg ttcgaaccac gcaagccagc
     1861 cttggacgat ggtcctctag agatgacctc cgcaatgtaa gcgcctttat ctactgttat
     1921 atccatcgcc tctaccaatg gaggagtcag gctgccgccg cggataccca gccatgccca
     1981 atgatgcacg ccatcttgga tcaagtcggg aaccaccagg cggacgatgc tcactgggat
     2041 ggcaaaccca atacccaggt tgctgcgtgt ggtgccaccg gtttcgatct gagcgttgac
     2101 accaataacc tcaccagcca agttgagcag cgggccgcca gagttacccg ggttgatagc
     2161 ggcgtccgtc tggatagact gcgggataga gaagggggtg agagctggga tggtgcgccc
     2221 caaggcactg atgatgccac gcgttagagt gccatccaat ccaaagggat tgccgatggc
     2281 gactaccgat tgcccaaccg ccagcgaatc catatcaccc aggggcaatg gaactgcatc
     2341 ctcgggcagg ttctctgcgc gaaccaccgc caggtcgcta ttcaaatctt caccgacaag
     2401 ctcggcgcga cgcaccacgc cactggagaa agtcacctca acctgttcgg cagcatgcac
     2461 gacatgagca ttggttacta tgtttccgtt ggtgtcgaat acaaagcccg atccctgccc
     2521 aaccggaagc aaatcgccgc tttgtctttg gtacacggtc acattcacca ctgatgggtt
     2581 cacccgcgtg tagaggtcaa ccagaacttc atcttcaccg gtaatttcta tggtagtagt
     2641 tctctccggg ggaaccggtg tgctgacgac aaccgtcggc ggctggtctg ggatagtctg
     2701 cacgttctct ggggtaaaga atcgacagga gaacagtagc acaggcaaca ataaaccgac
     2761 caggagaaca tagattgatc tacgatatcc ttgttgtttc atagttaatc tttctaacct
     2821 ccgataggtt agtatagtcc tctaagttaa atatttggtt agaaaatcat attaaaaaga
     2881 tcaaaagttg cgcagccagg caagtcttgt tcgactgcgc aagtcctaaa gtttattgct
     2941 gtacctcaat atctgatgga gactggacct cgatctcaaa aatgccttcg tactccagga
     3001 tttccccttg ggcacagatg gtcttcccat cataataatc ttctggtttg ttggagaagt
     3061 tatcccgata ttccacccag atcagcacaa cgaaacggtc gggcgatgga taatcttcgc
     3121 caatgttcag aaaagtaggt ttgcctcgcg attctttcgc gtagtgtgtg cctgccaccg
     3181 gaccacacac agtgcgctcc tcacccaggt attcgctggc atcagaccaa aggatatgat
     3241 cgcttggggt cggcgagctg gtgggcgtca tcggaatagc cgatgccgtt agcgttggtg
     3301 tgggcacgat gggtgttgct gtgggatcgg caatagttgg agggctagag gccgtcgggg
     3361 tcggtggttc aggcagggtt tccgtt
//
LOCUS       DBNHOH010000624         3258 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_935296, whole genome shotgun sequence.
ACCESSION   DBNHOH010000624 DBNHOH010000000
VERSION     DBNHOH010000624.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_935296"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="AC16V7_10925"
     CDS             <1..867
                     /locus_tag="AC16V7_10925"
                     /inference="COORDINATES: protein motif:HMM:NF037660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR6257676.1"
                     /translation="GLPFKMVDAVATGYAVITRIHLALDDLESAIEASLIAGRYVREL
                     PGLEPITITQIWDSEARVKFAQKKIDEAASLFLENNPCDVYDINCFGEFEQIIWARIL
                     LYRGRECKNVSDLLKAQDLINQMLELTKDLGLTSKTIELLVLNSLVLIEIETMDGALD
                     ALAEALWLAETGSYMRVFIDEGDSMKALLREAEARGIAPDYVSKLLAGFETDRVPVEI
                     TSVHSLIEPLSEREIEVLRLLKTELSGPDIADVLMIALSTLRTHTQNIYGKLGVSNRR
                     AAVRKAEELNLR"
     gene            1066..1785
                     /locus_tag="AC16V7_10930"
     CDS             1066..1785
                     /locus_tag="AC16V7_10930"
                     /inference="COORDINATES: protein motif:HMM:NF025687.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HIR6257677.1"
                     /translation="MNSETDTSKFADTSQRTAAIAVGIGLLASILGAVFVELTTNSLI
                     VADDAATTARNIMASEALYRAGIFGWVVAILGDMLRAWGLYVFFKRVNQSLALLSVWF
                     MLVHDAIFAASLVNLYFGSVFPSSADLLTVFEAEQLQALGMLVLNGFEYGFHMGLFFF
                     SFHLGVVGYLAYKSGFVPKILGVLMMAAFLSYLIDSFAFLFLPIYPELLNQFLMLPEI
                     IGEVAIAVWLAFKGGKGATDK"
     gene            1858..2520
                     /locus_tag="AC16V7_10935"
     CDS             1858..2520
                     /locus_tag="AC16V7_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007694271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HIR6257678.1"
                     /translation="MKNLQKMGGVAALYEAAAYLIALVGYLLVVGTMDVVDPVEKVST
                     LVNNQAFLYILNLIAYIFWGFFQIALALALYERLKAGSPALVQTATGIGLVWACVIIA
                     SGMISNVGMATVVDLYAKDPQHAATVWLAIESVANGLSGAGGETLGGTWVLLSSLVAL
                     RVGELPKVLNYFGVVLGLAGLLSTVPSLFMPVVFVFALGKIVWLIGLGIVMLRSRTSA
                     IA"
     gene            2732..2968
                     /locus_tag="AC16V7_10940"
     CDS             2732..2968
                     /locus_tag="AC16V7_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257679.1"
                     /translation="MNKSDHVNYEIKIKGCLEKRWAEWFEGLAFTHGCDGTTTLSGSL
                     PDQASLHSILLKIRDMNLTLISVTQVESKPDEEA"
     gene            3033..>3258
                     /locus_tag="AC16V7_10945"
     CDS             3033..>3258
                     /locus_tag="AC16V7_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257680.1"
                     /translation="MNIKSALVLLVKIVVLTVVMFILFSVGSGIFTAQLSATEMSPEE
                     SATSALGLLVVSIIDTMILTYFILRSRLSGL"
BASE COUNT          818 a          721 c          836 g          883 t
ORIGIN      
        1 ggtttaccgt tcaaaatggt tgatgccgtt gcgacaggtt atgcggttat aacccgaata
       61 catcttgcct tggatgacct tgaatcagcc attgaggcga gtcttattgc tggaagatat
      121 gttcgcgaat taccaggttt agagccgatt accatcacgc aaatttggga tagtgaggcg
      181 cgggtgaaat ttgctcaaaa gaaaatagat gaggctgcga gcttatttct tgagaataac
      241 ccgtgtgatg tatatgatat caattgtttt ggtgagtttg agcaaataat ttgggccagg
      301 attctccttt acagggggag agagtgtaaa aatgtgtccg atttattgaa agctcaagac
      361 ttgattaatc aaatgttgga gctgaccaaa gatttgggac tgacaagtaa aacgatagag
      421 ctcctggtgc tgaattccct cgtcctaatt gagatcgaaa caatggatgg cgcgttagat
      481 gctttagcag aagctttatg gcttgcagaa accggaagtt acatgcgcgt tttcatcgat
      541 gagggtgatt cgatgaaagc tttgctgcgt gaagctgaag ccaggggtat cgcacccgac
      601 tatgtctcta aattgctggc gggatttgaa actgatcgtg tcccggtgga aataacctct
      661 gtccattcac tcatcgaacc cctgagcgag cgagaaattg aagttctgcg cttgctcaaa
      721 acagaattat ccgggccaga tattgctgat gtgctgatga tcgccttgag cacattgcgc
      781 acccacacgc aaaacatcta tggcaaattg ggcgtgagta accgccgggc ggctgtccgt
      841 aaagcggaag aacttaactt gcgataatca atacatatca caactgccat tctgccctgc
      901 ctggatatgg gtcaacatag ctgcttccag gcagggcttt ttttgtccct cttggcttaa
      961 ctgatacagt aacataaatc atcacatgtg atgatgacat ctccacacat taatttgtat
     1021 attccaattg ataggatcga ttggaaaagc tagagaggag aaaaaatgaa ttcagaaaca
     1081 gatacctcaa aatttgccga cacatcacaa cgcacagctg caatagccgt aggcatcgga
     1141 ttactagcct caattctagg cgctgtcttc gtcgaactca ctacgaacag tttgattgtg
     1201 gcggacgatg cggcgacaac agccagaaat atcatggcat cagaggcgct atatcgcgcc
     1261 ggcatttttg gctgggtggt cgcaattctg ggtgacatgc tgagggcttg gggactttat
     1321 gtctttttca aacgcgttaa ccagagtctg gcgttgctct cagtatggtt catgttggtt
     1381 cacgatgcga ttttcgcagc ctcgttggtc aatttgtatt tcggttcggt ctttcccagc
     1441 agtgctgatc tcttgaccgt atttgaagct gaacagctac aggctctggg catgctagtc
     1501 ctgaatgggt ttgaatacgg attccatatg ggactattct ttttcagctt tcacctcggt
     1561 gtcgtgggtt atttagcata caagtcaggc ttcgttccca agattctggg cgtcttgatg
     1621 atggctgcat tcctcagcta tctaattgac agctttgcat tccttttctt gcccatctac
     1681 ccagagctat tgaatcagtt tctaatgctg cctgagatca ttggggaagt ggcgattgct
     1741 gtgtggcttg ccttcaaagg tggtaaaggc gcaacagaca aataactgtg ctcttgaatc
     1801 aacccgaata agactcatgc cgaaggaatc aatcaataac aataaaggag aaatgaaatg
     1861 aagaatttac agaagatggg cggcgttgcc gcactgtacg aggcagcagc ctacttgatc
     1921 gctctggtgg gttacctctt ggtagtcggc actatggacg ttgtcgaccc tgtcgagaaa
     1981 gtgtctacgc tcgtgaacaa ccaggccttc ctgtacattt tgaacctgat cgcctatata
     2041 ttttggggct tctttcagat tgccctggcg ctagcgctct acgagcgctt gaaggccggc
     2101 tcacccgcat tggtgcagac agcgactggc attgggcttg tctgggcctg tgtgatcatt
     2161 gccagcggca tgatctcaaa cgtcggcatg gctacagtcg tcgatctcta tgccaaagac
     2221 ccacagcacg ctgcgacggt ctggttggct attgaatctg tggcaaatgg attaagcggt
     2281 gctggtggtg agactcttgg cggcacatgg gttctgctat caagtttggt cgctttgcga
     2341 gtgggagagc ttcccaaggt actgaactac tttggtgtcg tgctcggttt ggcgggtctc
     2401 ctctcaactg ttccgtctct cttcatgccg gtggtgttcg tttttgcgct ggggaagatc
     2461 gtatggttga tagggctggg gatcgtcatg ttgcgcagca ggacaagcgc gatagcctga
     2521 atgacctaac gcgcaagatt gccgaggctc accggcatgc ttaaaaggcg cgaagtgaga
     2581 actgtccata tgaccgtgaa gcataatagt aaaaactaac ccaaattgca tctgactcga
     2641 ctgcgcctca gggcaaactg cgagggctag gcaattcaag tctaatggag cggcttgctg
     2701 agcgcaaaac gcaaaatggc tgaataaatc aatgaataaa tctgatcacg ttaattacga
     2761 aataaaaatc aaaggttgcc tcgagaagcg ctgggcggag tggtttgagg gactggcctt
     2821 cacccatggg tgcgatggca cgaccaccct ttccggttct cttcccgacc aagcttccct
     2881 acacagtatt ctgctcaaaa ttcgggatat gaacctgacc ctgatctcgg ttacccaagt
     2941 agagtcaaag ccggatgagg aggcttgagc caatcttttt gcagataaca tggaaccaaa
     3001 aaagaaaaca aaatgggtaa agggaaataa ctatgaatat caaatctgcg ctcgtcctgt
     3061 tagtaaaaat cgtcgttctg accgtggtga tgtttatctt gttctcagtc ggatctggca
     3121 tttttacggc acagctctca gcgactgaaa tgagccctga ggaatcagct acgtctgcac
     3181 ttggtctctt ggttgtcagt atcatcgaca cgatgatatt gacctatttc attctgcgct
     3241 cccgtttgag cggtctgc
//
LOCUS       DBNHOH010000625         2135 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_37418, whole genome shotgun sequence.
ACCESSION   DBNHOH010000625 DBNHOH010000000
VERSION     DBNHOH010000625.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_37418"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..889
                     /locus_tag="AC16V7_10950"
     CDS             119..889
                     /locus_tag="AC16V7_10950"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF048554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6257681.1"
                     /translation="MKLKDRVAIVTGGAMGIGWGICKVLSQEGAKIVVVDWDEKVGKN
                     TAEELNQGGSEAIFVLCDVSNEDQVKAMIEATLKRFGKIDILVNNAGIGVYKPVLETS
                     SADWDRCLAVNLKGQFLCSKYAIPHMQEIGKGAIVNISSVHSFQTVNGVAPYAASKGG
                     ITALTRNMAIDYGPAIRVNSIAPGWVLTPLIQSIFDSYPDPADQQRQVERRQVMKRIG
                     RPEDIGYAVAFLASDEASFITGTQLYVDGGLTAQLETW"
     gene            962..2119
                     /locus_tag="AC16V7_10955"
     CDS             962..2119
                     /locus_tag="AC16V7_10955"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HIR6257682.1"
                     /translation="MSDQKVQYNIIQDDLSGFADKGPAFVTIGECMVRDTPTDMQRLE
                     MTRQVHISLAGSEYTLAMTLARLGIPSAYITRLPDNPYGWMVRDTARSQGIHTDYIVW
                     APKAEPIGRLLYELGRTPRKSVAYYQRMCSAASRLSPGMVDWRSALRDCKLFHTSGIT
                     FGLANHSKYEHNYLLDTFHEAMANRQPDTLVGLDFNYRSTLWKPEQCKAVMTPLISDY
                     IDILITTIEDMAALYGIGCGQYSAEQIDKGDIGELTDSDIKAFAEQVGELFKTRIVAI
                     TIRYPDTFEQHRWEAAAMDAEGHFFRSPAVKPMVLLDRLGGGDTWNGGFYYGLLTTGF
                     NPDGIAKGVLVGDAATRIKQTLMFDLPIVTRHEVQDLMKADVLGGGKRVAR"
BASE COUNT          512 a          575 c          584 g          464 t
ORIGIN      
        1 tggcttcggt ggtttgcgca tcgaagataa cgtagcggtc ggcgtcgaag gcccggtgct
       61 gctctcttcg ccacgaaagc catggtaagg taatttagga caaaattgga ggtcaaccat
      121 gaaattaaaa gatcgtgtcg caattgtcac cggaggggcc atgggcattg gctggggtat
      181 ctgcaaggta ctcagtcagg agggggcgaa aattgtcgtg gtcgactggg acgagaaggt
      241 tggcaaaaat accgccgaag agctcaatca gggtggcagc gaggcgatat tcgtcctgtg
      301 cgatgtatcc aacgaggatc aagtcaaagc tatgatcgaa gccacgctga aacgctttgg
      361 taagatcgat atcctggtta ataacgctgg catcggtgtc tacaagccgg ttctagagac
      421 cagcagcgcc gactgggatc gttgcctggc ggtaaacctc aaaggccagt tcctttgctc
      481 caaatatgcc atcccgcaca tgcaggaaat cggcaaagga gccattgtca acatctcatc
      541 ggtgcattct ttccagacgg taaatggcgt tgctccctat gctgcctcca aaggtggaat
      601 cactgcccta acgcgcaaca tggctatcga ctacggaccg gctatccggg tcaactccat
      661 cgcacctggc tgggtgctca cccctctgat ccaatcgatc tttgacagct atccagaccc
      721 agctgaccag cagcgccagg ttgagcggcg ccaagtgatg aaacgcatcg gtcgcccgga
      781 ggatatcggt tatgccgtag ctttcctggc tagcgacgaa gcttccttta tcaccggtac
      841 ccaactatac gtggatggcg gcctgacagc tcagttagaa acctggtaat cagaaaaggt
      901 gcgatgcgct tccggagtgc gtcatagctg aagtacagat taattaagga gaagctcatt
      961 tatgagcgat cagaaggtcc aatataacat catacaggac gacctgagcg gcttcgccga
     1021 taaagggcca gcatttgtta ccattggcga atgcatggta cgagatacac ccacagatat
     1081 gcagcgcctg gagatgaccc gccaggtaca catctcactg gcaggcagcg aatatactct
     1141 ggcgatgacc cttgctcgcc tgggcatccc atccgcttat atcactcgcc tgccagacaa
     1201 tccctacggt tggatggtac gtgacacggc acgaagccag ggcatccaca ccgactacat
     1261 tgtttgggcc cccaaggctg agccaattgg tcgtctgctc tatgagctag gccgcacccc
     1321 tcgcaagagt gtagcctatt atcagcgcat gtgttcagcc gccagtcgtc tatccccagg
     1381 tatggtggac tggagatctg ccctgcgaga ctgcaagctg ttccacacct ctggcatcac
     1441 cttcggactg gcaaaccata gcaaatacga gcacaactac ctactggata ccttccacga
     1501 ggcgatggca aatcggcaac ctgataccct ggtgggtctg gattttaact atcgctccac
     1561 gttgtggaag cctgagcaat gcaaagccgt catgacgcct cttatctccg attacattga
     1621 catcctgatc acgacaatcg aggatatggc cgctctgtac ggtattggct gcgggcagta
     1681 cagcgccgag caaattgata aaggagatat cggcgagctg accgactccg acatcaaggc
     1741 cttcgccgag caggtaggcg agctgttcaa gacccgcata gttgccatca ccatccgcta
     1801 tccagacacc tttgaacagc accgttggga ggcggctgcc atggacgctg aggggcattt
     1861 cttccgctcg ccggcagtca agcctatggt actgctggac cgtttgggcg gcggtgatac
     1921 ctggaacggc ggattttact atggtttgct gactaccggt ttcaaccctg atggtatcgc
     1981 aaaaggcgtg ttggtcggag acgctgccac acgtattaag caaactttga tgttcgactt
     2041 acccatcgtc acccgccacg aagtgcagga tcttatgaag gctgatgtgc tcggcggcgg
     2101 caagcgggtc gctcgctgag ctaggcaata aaagg
//
LOCUS       DBNHOH010000626         1995 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_678844, whole genome shotgun sequence.
ACCESSION   DBNHOH010000626 DBNHOH010000000
VERSION     DBNHOH010000626.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_678844"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="AC16V7_10960"
     CDS             <1..352
                     /locus_tag="AC16V7_10960"
                     /inference="COORDINATES: protein motif:HMM:NF019829.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HIR6257683.1"
                     /translation="FIRLYQGQYEGYLQQGDNEKAVSLEQCFVLRFWLTHQFQSFPRT
                     DPNLHPALLPPDWIGFKARELFENYRALLESHANQYVDDVLGAGNTNKESVQSIPLEN
                     RQTALDIPAKEVSV"
     gene            411..1457
                     /locus_tag="AC16V7_10965"
     CDS             411..1457
                     /locus_tag="AC16V7_10965"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6257684.1"
                     /translation="MDVLTDKKTTFEEIIYEKENWVARITINRPYNYNAYSTNCLEEL
                     AIAFRQASFDDEVAVIVYTGAGDKAFCTGGDVKEYQDRYTRSPHDYWKYMRLFGAYIE
                     SIVNTGKPVIARLNGMAVGGGNESQMACDLAIIAEHGWIGQVGSGVGSVAAGGATQWL
                     PIMVGDRRARYMLMTNRRIPPYQALEWGLVNEVVPSVKADGEFINHATPEQISMASKG
                     KDGYSIDLSLLDEAVNKLAQELVDKFPECIRYTKEQTNFWKNLAWHQTIGHARDWLSI
                     HYTSWEPLEGMTAFVEKRPPQYRLLRERAAQGKSSEFIWGPYSQTCSNCGAKALPEEF
                     TFCGECGNPLDGAS"
     gene            1498..>1995
                     /locus_tag="AC16V7_10970"
     CDS             1498..>1995
                     /locus_tag="AC16V7_10970"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6257685.1"
                     /translation="MSSEGNEFITLEIHDGVATLSLNRPPLNVMNLAMLQQMEATLEK
                     AASDTSIRVLVVQAKGKAFSAGVDVADHTADKVGEMIPLVDRVCKALADFPVPTLAAV
                     HGHALGGGCELVLCCDLAVMAAEASIGQPEIYLAALAPIAALRLPYLVGYRMAADILL
                     TGRRLT"
BASE COUNT          516 a          515 c          558 g          406 t
ORIGIN      
        1 gtttattagg ttataccagg gtcaatacga gggttacctg caacagggag acaatgagaa
       61 agccgtctcc ctggaacaat gctttgtact gcggttctgg ctgactcacc aatttcaatc
      121 cttcccgcgc accgacccga acctacaccc agcgctgctg ccccccgact ggattggatt
      181 caaggcgcgc gagttgttcg agaattatcg agcgttgctc gaatcacacg ctaaccaata
      241 cgttgatgat gtactgggcg cggggaacac caacaaagag agcgtccaat caatccccct
      301 ggaaaatcgc cagactgcgc tagacattcc ggcgaaagaa gtgtccgtgt aaatgggaag
      361 ggacacagga aatagcagaa gttcaatgag tgctgagctt ggaggccaca atggatgtgc
      421 ttacggataa aaaaacaaca ttcgaggaga tcatttacga aaaagaaaat tgggtagccc
      481 gtatcacgat taaccgcccc tataactaca atgcctacag caccaactgc ctggaagaat
      541 tagcaatcgc attccggcag gccagctttg atgacgaggt ggctgtaatt gtatacaccg
      601 gagcaggaga caaggcattt tgcaccggcg gagacgtgaa agaatatcag gaccgctata
      661 cccgatcacc gcacgactat tggaaatata tgcgcttatt tggcgcttac atcgaaagta
      721 ttgtaaacac cgggaaaccc gtcatcgccc gtctgaatgg catggccgtg gggggtggca
      781 acgagtcgca gatggcttgc gatctggcga tcatcgctga gcacggctgg atcgggcagg
      841 tgggcagcgg ggtaggcagt gtggctgccg ggggagcaac ccaatggtta ccgattatgg
      901 taggagaccg gcgcgcccgt tacatgctga tgacaaaccg ccgcataccc ccgtaccagg
      961 cgttggaatg gggtttggta aatgaagtag tgccctcggt gaaagcagac ggcgaattca
     1021 ttaaccacgc cacgcccgag caaatctcga tggcatctaa aggcaaggac ggctacagca
     1081 tcgatttatc tttgctcgat gaggcggtga acaaactggc gcaggagctg gttgacaaat
     1141 tcccagaatg cattcgatac acgaaggaac aaaccaactt ctggaaaaac ctagcctggc
     1201 accagaccat tgggcacgcc cgcgattggc tatcgatcca ctacacgtcc tgggagcctc
     1261 tggagggcat gactgccttc gttgagaagc gtccccctca gtatcgtcta ttgcgcgagc
     1321 gcgctgccca aggtaaaagc agcgaattta tttggggtcc atatagccaa acgtgctcca
     1381 actgtggggc taaagccttg ccggaggaat ttaccttctg cggcgagtgc ggtaaccctc
     1441 tggatggggc aagttaggca agcagtcaga tcaaagtaac gaaccaagga tagcaacatg
     1501 tcttccgaag gaaatgaatt catcacactc gaaatacacg acggcgtcgc cacactgagc
     1561 cttaaccggc ctcccctaaa tgtgatgaac cttgcgatgt tacagcaaat ggaagccacc
     1621 ctggaaaagg cagcttccga tacctccata cgcgtgctgg tagtgcaggc aaagggcaag
     1681 gccttttcag cgggcgttga tgtagcagac cacaccgccg acaaagtagg tgagatgatt
     1741 cccctggtag accgggtatg caaagcgctg gcagattttc ccgttcccac actggctgcg
     1801 gtacacggac acgccctggg cggtggctgt gaattggtgt tgtgctgtga cttggcggtc
     1861 atggcagccg aagccagcat cgggcaaccc gagatctacc tggcagcatt ggctccaatt
     1921 gcagcgttgc gattgccata cctggttggt tatcgcatgg ctgccgatat cctgttgacg
     1981 gggcgccggc taaca
//
LOCUS       DBNHOH010000627         2209 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1704504, whole genome shotgun sequence.
ACCESSION   DBNHOH010000627 DBNHOH010000000
VERSION     DBNHOH010000627.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1704504"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /gene="sucC"
                     /locus_tag="AC16V7_10975"
     CDS             <1..991
                     /gene="sucC"
                     /locus_tag="AC16V7_10975"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HIR6257686.1"
                     /translation="GGIRLAKNPDEAEELAAQILAMEIKGLPVRKVLVDEAAQIEKEI
                     YLGITNDRTARKPVMMSSSAGGVEIEEIARKEPQKIIKTHIDPLLGLGDYQARDIAAG
                     IDLPREHWKSFGMIAHGLWKAYLDCDATLAEINPLVITSDNRLLALDGKMVLDDNALF
                     RHPDLAEMRDLDAEAPSEIEARKYGLSFIKLDGNIGCMVNGAGLAMTSMDIIKLFGGE
                     PANFLDIGGGASADKVTAALRIILADPNVRAVLFNVFGGITRGDEVARGILVALDEVK
                     TKVPMVIRLVGTNAEEGRQILADANMITAETLADAARKAVAAAQAQPTKGAVS"
     gene            988..1851
                     /gene="sucD"
                     /locus_tag="AC16V7_10980"
     CDS             988..1851
                     /gene="sucD"
                     /locus_tag="AC16V7_10980"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIR6257687.1"
                     /translation="MSILVDKNTRLLVQGITGNEGLFHTQQMVEFGTNVVAGTRPGKG
                     GEWVLDGKIPVFDSVKIAVEATGANTSVIFVPARGAADAMFEATDAGLSLVVCITEGV
                     PVQDMMRVRSYMDQKGVRLVGPNCPGLLTPGATKVGIIPGDISIPGNIGVVSRSGTLT
                     YEVLFALKQLNMGVSTCVGIGGDPINGTSFIDCLDLFEEDPNTDKIVLIGEIGGSDEE
                     RAAEYIASRMTKPVVSFIAGQTAPPGKRMGHAGAIVEGGSGLASDKIRALQAAGVSVA
                     RHPEEIPDLLQ"
     gene            complement(1935..2168)
                     /locus_tag="AC16V7_10985"
     CDS             complement(1935..2168)
                     /locus_tag="AC16V7_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR6257688.1"
                     /translation="MSDTFDEIKTIIVDLLGVDGDKVTLDARFREELEADSLDLVELI
                     MAFEDKFGGEISDEDAQTITTVGEAVKYIDTHM"
BASE COUNT          572 a          528 c          589 g          520 t
ORIGIN      
        1 tggaggcata agactggcca aaaacccaga tgaagctgag gaacttgcgg cgcaaatatt
       61 agcaatggag atcaaaggcc ttccagtgcg caaagtcctt gttgatgagg ctgcacaaat
      121 cgagaaagag atctatctag gcatcaccaa tgaccgtact gcccgcaaac ctgtgatgat
      181 gtcttcttca gctggaggcg tagagatcga agagatcgcc agaaaagaac cccaaaaaat
      241 catcaagact cacatagacc cgcttttggg actaggagac tatcaggcgc gtgatatcgc
      301 tgccgggatc gacctgccgc gcgagcattg gaaatcattt ggaatgattg cccatggcct
      361 ttggaaagcc taccttgatt gtgacgccac gctggcagag atcaaccccc tcgtaatcac
      421 gtcagacaac cgcttgctgg cactggatgg caaaatggtg cttgacgata acgctctctt
      481 tcgtcaccct gacctggcgg agatgcgtga cctggatgcc gaggctccct ccgaaatcga
      541 agcgcgaaag tatggcctgt ctttcataaa gttggatgga aacataggct gcatggtcaa
      601 tggtgctgga ttagcaatga cctccatgga tattatcaag ctgtttggag gagagccagc
      661 aaactttctt gatattggcg gaggtgccag cgctgacaag gtcaccgcag ctttgcgcat
      721 cattctagct gacccaaacg tgagagcggt gctttttaat gtcttcggag gtatcacgcg
      781 aggcgatgaa gttgctcgag gcattctcgt cgctttggat gaggtcaaaa ccaaggtacc
      841 catggttatt cgcttggtgg gcaccaacgc tgaagaaggc cgacagatac ttgccgatgc
      901 caatatgatc acagccgaga cgctagctga cgctgctcgc aaagctgtcg ctgccgcaca
      961 agctcaacct acgaaagggg cggtatcatg agcatattag tcgacaaaaa cacccgcctg
     1021 ctggtgcagg gtatcacggg gaatgaaggg ctttttcaca cacaacagat ggtcgagttt
     1081 ggcaccaatg tagtagctgg aacaagaccc gggaaaggtg gggaatgggt gttagatgga
     1141 aaaatcccgg ttttcgactc ggtgaaaatc gctgtagaag ccaccggagc aaacaccagc
     1201 gtgatcttcg tcccggcccg cggcgcggca gacgccatgt ttgaagctac agatgcaggt
     1261 ctctcattgg tggtttgcat cacagagggc gtccctgtcc aggacatgat gcgtgtgcgc
     1321 agctatatgg atcagaaagg tgtgcgccta gttggaccga actgccccgg gctgctaacg
     1381 cctggtgcaa ccaaggtggg catcatccct ggggatatat ctatacccgg taacattggt
     1441 gtggtgtcgc gttctggaac gcttacctat gaggtgcttt ttgcgctcaa gcaattaaat
     1501 atgggggtga gcacgtgtgt gggtattgga ggcgatccta tcaatggcac aagcttcatc
     1561 gactgtttag atctttttga agaagaccct aatacagata aaatcgtcct gattggggag
     1621 attggcggtt cagatgagga acgtgcggct gaatacatag caagccgtat gaccaaaccc
     1681 gtggttagct ttatcgccgg gcagacagcc ccaccaggta aacgtatggg gcatgcgggt
     1741 gccattgtgg aaggtggatc cggattggct agcgataaaa tcagagcctt gcaggctgct
     1801 ggcgtaagtg ttgctcggca tccagaagaa ataccagatt tactccaata agcttttccc
     1861 ccttaaaata atttgccgga aatagatatg tgcggtgcaa tcgcaccaca gatatctatt
     1921 tccggcagtt tttgttacat atgagtatcg atatatttga ccgcctcacc aacggtcgtg
     1981 atcgtttggg cgtcttcgtc agaaatctct cctccaaatt tatcttcaaa ggccataatt
     2041 agctccacaa gatcgagcga atcagcctcg agctcctcac gaaagcgcgc atctaaagtg
     2101 actttatcac catcaacccc aagcaggtcg acgataatgg ttttgatttc atcaaatgta
     2161 tcggacatat agcatgcctc cttcggttaa ttataatcct gccaatttt
//
LOCUS       DBNHOH010000628         2096 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1319752, whole genome shotgun sequence.
ACCESSION   DBNHOH010000628 DBNHOH010000000
VERSION     DBNHOH010000628.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1319752"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="AC16V7_10990"
     CDS             complement(<1..311)
                     /locus_tag="AC16V7_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257689.1"
                     /translation="MEHNNTQIEIVDKPPVNREIEIIDSDQQPESEYAAGLKHYSSAW
                     VLTWTGIFGSFLAILYFAYLGYRFDAWQLQIVTLSLVGLMLTAVISTVLIRRNKPKGI
                     W"
     gene            complement(298..>2096)
                     /locus_tag="AC16V7_10995"
     CDS             complement(298..>2096)
                     /locus_tag="AC16V7_10995"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR6257690.1"
                     /translation="KDPLWTAPYVDATGLGLVTTSAAPVYSRGGELIGVVGLDLTLSD
                     LIANVEATRFLKSGYTFLIDHTGHTIALPEQGYKDFLGRAPEPGEIYIDLNQSHAQFK
                     PIISRMVVGDIGLEHLNIGNRSIFVAFAPLNSTGWSLGSVIEADDILNSISSLKGQLN
                     DTTRYTVLTRILPITTAIFILIVILGLFFTNRLVVPIQKLAAEAQKIGAGEWDITVPP
                     TTNDEIGVLAGAFKAMAEQIHVFIRDLEKRVAERTRDLERRNTQLQVAAEIAREATAV
                     HELDELLELAVNLIRGRFGFYHAGIFLLDENKEFAVLSAATGEAGREMLARGHKLRVG
                     QTGMVGYATKRGQPRIALNVESDPTHYRNPLLAETRSEMTLPLKVGSVIIGALDVQSH
                     FPDAFSQDDITIMQVLADQLAIAIENARLIQEAQEHVRQLEKLYGTYSKKAWNELRES
                     SITLGYQYDSSGVKPIYRISNGHNTQNVSEAPPPSIQIPIQIRGQAIATLDIWHEGDK
                     LDAETHALLNSIAERLSLALEGARLFEEEHQRAEYERVVGDVTSQFRATLDIQTVIQT
                     AAREFRKVLNLEEAEVRLTNTQIGEKPDHGTQ"
BASE COUNT          501 a          495 c          512 g          588 t
ORIGIN      
        1 aaccagatgc ctttaggttt gtttctgcgg atcaataccg tactgataac tgccgtaagc
       61 atcagcccga cgaggctcag tgtcacaatt tgaagttgcc aggcatcgaa acgataaccc
      121 aggtaggcga agtagagaat tgctagaaat gagccgaaaa tacctgtcca ggtaagtacc
      181 caggcactcg agtagtgttt taacccagca gcatactcag actccggttg ttgatcggaa
      241 tcgatgattt cgatctctct atttactgga ggtttatcaa ctatctcaat ctgggtatta
      301 ttgtgttcca tggtccggtt tctcaccaat ttgagtgttg gttaatctaa cctcagcttc
      361 ttcaagatta agtactttcc tgaactcacg agcagctgtt tgtataacag tctggatgtc
      421 aagagtcgct ctaaattgac tggtgacatc acccacaaca cgttcatatt cggcgcgttg
      481 atgttcctct tcaaacaatc gggctccttc aagcgccaag ctcaatcgtt ctgcgatgga
      541 gtttaataat gcgtgtgtct ctgcatccaa tttatcacct tcgtgccaga tatcgagggt
      601 tgctatggct tgtccgcgaa tttggatggg aatctggatg gatggcgggg gtgcttcaga
      661 gacattctgg gtattgtgtc catttgagat tcgataaatg ggtttcaccc ctgatgaatc
      721 gtattggtaa cccaaagtaa tgctcgattc tcttagttca ttccaggctt ttttgctgta
      781 tgttccgtat aacttctcta attgcctgac atgttcctga gcttcctgga tcaaacgggc
      841 gttttcgatg gcaatagcta attgatcggc taaaacctgc atgattgtga tgtcgtcctg
      901 gctgaaagcg tcaggaaaat ggctttgaac atctagtgca ccgatgatga cgcttccaac
      961 cttgagtgga agagtcatct ccgagcgagt ttccgccagt agtggattgc gatagtgggt
     1021 tggatcgctt tctacattga gcgcgatacg gggctgcccg cgtttggtag catagccaac
     1081 catgccggtc tgaccaaccc gcaatttatg accgcgggcg agcatttctc tgcctgcttc
     1141 acctgtggcg gcgcttagta cagcaaattc cttgttttcg tcgagcagga atatgcctgc
     1201 gtgatagaag ccaaagcgcc cacggatcag attcacagcc aattccaaga gctcgtccag
     1261 ctcatggaca gcagtcgctt cacgcgcaat ctctgcggca acctggagct gggtattgcg
     1321 gcgttcaagg tcacgggtgc gttcggccac acgcttctcc aagtcgcgaa tgaatacgtg
     1381 tatttgctct gccatagcct taaatgcgcc ggctaaaacg ccaatctcgt cattggtggt
     1441 aggtggaacg gtgatatccc attcaccagc gccgattttt tgcgcctcgg ctgccagttt
     1501 ctggatggga actaccaacc ggttggtaaa gaataatccc aggatgacga tcaggataaa
     1561 tatcgctgta gttatcggta atattcgggt cagcaccgtg tagcgagtgg tgtcgttgag
     1621 ctggcctttc aatgaactga ttgagtttag gatatcatca gcctcgatta cactacccaa
     1681 gctccaccca gtactgttca agggtgcaaa tgctacaaag attgaacggt tgcctatgtt
     1741 tagatgttca agaccaatat ctccaacaac cattctgctt atgattggct tgaattgtgc
     1801 gtggctctga ttaaggtcaa tgtagatttc acccggttct ggagctcgcc caaggaagtc
     1861 tttgtaaccc tgttcgggaa gggcgatggt gtggcctgta tgatcaatca agaaggtata
     1921 gccgcttttt aaaaagcggg ttgcttctac attggcgatt agatcactta atgttaggtc
     1981 aagtccgaca accccaatca attccccacc acgactgtat accggcgccg ctgaagtagt
     2041 gactaaccct aatccagttg catcaacata tggtgcggtc cataatggat ccttgt
//
LOCUS       DBNHOH010000629         1654 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_294131, whole genome shotgun sequence.
ACCESSION   DBNHOH010000629 DBNHOH010000000
VERSION     DBNHOH010000629.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_294131"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="AC16V7_11000"
     CDS             <1..548
                     /locus_tag="AC16V7_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257691.1"
                     /translation="ANELSEDTQHNDSSSDELAAEAINLTDASVESINAEAVRLRMGS
                     VGQLTAQETELRLSAAQQITTLAVNMEESAAAVIMTDQADISDSIIGAIQTNHVSATN
                     NIFGLVYAQTAEIEEGRAGLIVTRQIQGESVKAAVLLAGRVEGPVEVALDTPRALLAG
                     LAAGVGAGLVLLIGSLSRRRK"
     gene            683..1327
                     /locus_tag="AC16V7_11005"
     CDS             683..1327
                     /locus_tag="AC16V7_11005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HIR6257692.1"
                     /translation="MEKIVFKAKHREVIGKQVKALRREGRLPAILYGKSIQPIPVSLD
                     YRDVSRILPAISSSHLILVDVDGEEYNALIREKQLHPVQGTVVHLDFMVVSLTEKLRA
                     YVSINIEGEAPASRDFNGMVVTGLEEIEVESLPGDLPERIMVDISSLDEIGSAIHVRD
                     LQLPENVKILTDLDEMLVIVTAPEAEEVVEEEEEELEEPEIIEKGKKEEEEEEE"
     gene            complement(1387..>1654)
                     /locus_tag="AC16V7_11010"
     CDS             complement(1387..>1654)
                     /locus_tag="AC16V7_11010"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6257693.1"
                     /translation="TSYRVTPIVGVIPWPYPLKLETKEVSRAFTIPLTWLADPKNREE
                     RPRTLPGPFEPINVIYYKSYDGETLWGASARFTLELINVLFDPE"
BASE COUNT          447 a          337 c          455 g          415 t
ORIGIN      
        1 gtgcgaacga gctttctgag gatactcaac acaatgatag ctcatccgat gagctggctg
       61 cagaagcgat taatcttacg gatgcatccg tcgagtcgat caatgccgaa gcagtccgct
      121 tgaggatggg ttctgtaggc caattgaccg cacaagagac cgaacttcgc ctctcagctg
      181 cccagcaaat cactacgctg gctgtaaata tggaggagtc cgcagcggca gtgatcatga
      241 cagaccaagc cgatatctcg gatagcatca tcggtgcaat acagaccaac catgtctcag
      301 ccacgaataa catattcgga ttggtttatg cgcaaactgc cgagatcgag gaaggacgcg
      361 cgggtttgat tgtgactcgt cagatacaag gcgaatctgt taaggcggca gtattattag
      421 ctggccgggt ggaaggtcca gtagaagttg cgctggatac gcccagggca ttgcttgcag
      481 ggctggctgc tggggttggt gcaggactag tccttctgat tggctctttg agccggcgtc
      541 gtaaatgagg ttgaacaaca gccagattgg tacagaatac ctggatggct gttaatatcc
      601 accggcggag gccggatatt atcccaagga gtgatggtaa cctggggaat cctgggtgag
      661 aggcgctcca aggaaggaag atatggaaaa aattgttttt aaagccaagc atcgcgaggt
      721 aattggcaag caagtaaaag ctctgagacg cgaagggcga ttacctgcaa ttctttatgg
      781 gaagagcatt caaccgatcc ctgtttcgct tgattatcgt gacgttagtc ggatactacc
      841 tgcgatttcg tcctcacatt tgattctcgt agacgtagac ggggaggaat ataacgcgtt
      901 aatccgtgag aagcagcttc atccagttca gggcactgta gtgcatttgg attttatggt
      961 tgtttcgttg accgagaagt tgcgtgccta tgtttctatt aatattgaag gggaagcccc
     1021 tgcaagtaga gatttcaatg gcatggttgt cactggtctc gaagagattg aagtcgaatc
     1081 cttgcctggc gatttaccag agcgcattat ggtagacatt tcttcgctgg acgagattgg
     1141 ttcggcaatc catgtgcgtg atcttcaact accagagaat gtaaagatat taaccgatct
     1201 cgacgaaatg ttggtgattg taactgctcc tgaggctgaa gaagttgtcg aagaggaaga
     1261 ggaggaactc gaggagccgg aaattataga gaaaggtaaa aaagaagaag aggaagaaga
     1321 ggaataatat tgaagatggg gtatatgcgg tgtatctaca gccacatata ccccattttt
     1381 ctctgcttat tctggatcga ataatacatt gatcaattca agggtaaatc ttgcgctggc
     1441 accccatagc gtttccccat catacgactt gtaataaatc acgtttatcg gctcaaaggg
     1501 gccgggtaaa gtccgcgggc gctcttcacg attttttggg tctgccaacc aggtaagggg
     1561 gatagtaaaa gcacgactaa cttccttggt ttctaattta agtggatatg gccaagggat
     1621 aacgccaacg attggtgtaa ctcgatagct ggta
//
LOCUS       DBNHOH010000630         7017 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_806718, whole genome shotgun sequence.
ACCESSION   DBNHOH010000630 DBNHOH010000000
VERSION     DBNHOH010000630.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7017)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7017)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7017
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_806718"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="AC16V7_11015"
     CDS             <1..787
                     /locus_tag="AC16V7_11015"
                     /inference="COORDINATES: protein motif:HMM:NF023401.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 4 glycosyl hydrolase"
                     /protein_id="HIR6257694.1"
                     /translation="SPRFIPHYRAVARRAREKLRITAAGLNHFTWMLDVRHPTNDQDL
                     YPLFTRRWGELPESFEPLTRRVFADFGLFPIPGDEHLCEYLPWMSDPLTRPWEKFDLS
                     LYEWDLWANRRLEEQEQIRRLASGEMSLKRLQEQDSEGAVEIIESVTGAGTQQALAVN
                     LPNRGYIQNLPEGAIVEVPGVLSDDGVQGVEVGSLPAPIAELCRREISAAQLCVDAAV
                     HGDRQAALQCLLLDPVINDMDLAKQVLDDYLRAYQDVLPQFWD"
     gene            804..2267
                     /locus_tag="AC16V7_11020"
     CDS             804..2267
                     /locus_tag="AC16V7_11020"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR6257695.1"
                     /translation="MPRIPLLLKYILVLCCLPIALSGCGLRELYQEKPDEGKNAASAG
                     KNAGSSARPTATLKPTATNTPVPTSTLGVEVADLQGVSIVFWHAWTGAPGDVIQSLVD
                     EFNRDNQWGISVEAVAQGSYDQVSEQVSAALENDQPPDVTTAYGYQALGWISPGLSLI
                     DLAPYVDDPLWGLDEQAQSDYYPIFWEHDLQNGERTGIPAQGSAQLLYYNNTWAKELG
                     FENPPATPEEFKAQACAANQTYKQDSDPKNDQKGGWIISTEYSAVLGWIYSFGGEIER
                     ADGNGYRFNNAPVKDAFTYMRELYEGSCSWLSESQFPEDDFATRQGLFAAGSVAGILY
                     QEEAFVEASSKDEWSVIAFPSLNGEPVISAYGPSFQVLQSTPEEQLASWLFIRWLTEA
                     EPQAKLAEVSGYYPVRASSVEMMGILPAAHPQWKSAVDLLPYARPEPALGSWRIVRWA
                     LSDAATQLFRFYFESDQLPALIKLLEETSNDLHAGLK"
     gene            2283..3056
                     /locus_tag="AC16V7_11025"
     CDS             2283..3056
                     /locus_tag="AC16V7_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR6257696.1"
                     /translation="MTYEFILTEVHDRVGLVRLNRPKALNALSSGLTRELMDALLAFE
                     ADEAIGAIVITGDERAFAAGADIKEMSEASAVEMLKRDMIGNFDAIQKVTKPVIAAVS
                     GWCLGGGNELAMSCDMIVASETARFGQPEINLGVIPGAGGTQRLTRAVGKALAMEMVL
                     NNRTLTASEALHYGLVNRVVPVERYLQEALELATEIAARAPLAVRMGKEAVNHAFETT
                     LAEGMADERRAFYFLFASQDQKEGMAAFTEKRKPQWKGM"
     gene            3316..3807
                     /locus_tag="AC16V7_11030"
     CDS             3316..3807
                     /locus_tag="AC16V7_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257697.1"
                     /translation="MQTKNIVLTTIAILLALSVIAASVLPATTSTIKKGETVEFYSGR
                     AGVSFTDSQFTGAVRLARIDDSKIKATNHPKFKHDLLSVRFTKSNGEKVTHVLGAVYV
                     FFIVNRSEVSAWEKGELAVYVYDSWKNEWQECYSFLVNDSNGKSSLACRMRIFSTYGV
                     AKK"
     gene            complement(3829..5322)
                     /locus_tag="AC16V7_11035"
     CDS             complement(3829..5322)
                     /locus_tag="AC16V7_11035"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6257698.1"
                     /translation="MRFFLPHTIQARLILSHLLVSLASVALIASYAGQILYSAVRQQA
                     EHRYEVLSFAAARDVEGSLIDYLSGQSSNERVRQSLARIFSSQPEVHYTIYLPDGIPL
                     MDSSGNLPPAADPQTRPELWEALSRQTGEGEVFRRSWQGEEFLYLVTRIQREDQVYGL
                     LSINIPLEAVLKPARSSLLLLIGFALIIGLVVSVAGFFLARSLAEPIEQITHAAESLT
                     SDKMSARFDSSTNLQEINLLAVAFNNMAARLESYVNELRSFVANASHELRTPLTSIKL
                     RVEALRNGALDDPPVSERFLGEVESEVDRMSSMVNDLLDLSRIETGLDPSRHEPVDLA
                     TITYDVFDAFKVRAERAEIQLGSSIHPDLPPVMGNEEQLRRMLYNLVDNAVKYTARNG
                     RVEISLEVNEQGGNLLLKVSDTGYGIAPVHLPHLFERFYRVEATRPRYGPSTGSGLGL
                     PIAKSIVEAHGGKIGVDSQLGQGTVFWVELSTLKMPISRANMNGTGE"
     gene            complement(5323..6021)
                     /locus_tag="AC16V7_11040"
     CDS             complement(5323..6021)
                     /locus_tag="AC16V7_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6257699.1"
                     /translation="MTRIMLVDDEPLITDSLTYSLRREGFDVKSVGDGSIALQEILDY
                     APDLVVLDVMLPGMNGLEICRRLRAQSTIPVIMLTARGEEIDRVLGLEVGADDYLAKP
                     FSFRELLARVRSILRRVELDRQVSMMQSINLGNLTLDSTARRVFKSGQEVQLSAREFD
                     LLSCLMKNAGRALSREDLLGEVWGSDWIGDQRTLDVHVRWLRLKVEDDPASPKYIQTV
                     RGHGYRFAGPEELE"
     gene            6261..>7017
                     /locus_tag="AC16V7_11045"
     CDS             6261..>7017
                     /locus_tag="AC16V7_11045"
                     /inference="COORDINATES: protein motif:HMM:NF046195.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HIR6257700.1"
                     /translation="MRRVHPFSSIFGFLATALLLAGLAMLLVRDGGIAFSPGKLSAKS
                     QPGVSLQGFTSHAEIEPDCSRCHQPLKTTQDTLCLECHSSVAGQIHRTDGAHSLIADI
                     RRCFACHTDHQGEDFDPTQAAFALFDHSKTRFSLLRHQVNYDASPMECLACHQYEGKM
                     AFSDSYCTLCHAAENMDFAVQHSQQYGANCSYCHDGGDRLADFDHQTTAFPLEGKHAQ
                     AYCADCHAPQELAVGRGGGGLSSSQPVGAPSDCV"
BASE COUNT         1637 a         2002 c         1922 g         1456 t
ORIGIN      
        1 ttcgccgcgc ttcatcccgc attaccgggc ggtcgctcgc cgggcacgcg agaagctgcg
       61 catcaccgcg gccgggttga accacttcac ctggatgctc gacgtacgcc accccacaaa
      121 tgaccaagac ctgtacccgc ttttcactcg ccgctggggc gagctgcccg agagcttcga
      181 gccgctcacc cgccgcgtat tcgccgattt cggtctattc cccatccctg gagacgagca
      241 cctatgcgag tacctgccct ggatgagcga cccactcaca cgcccctggg agaagttcga
      301 tctcagcctg tatgagtggg acctgtgggc aaaccgccgc ctggaagaac aggagcagat
      361 tcgccgcctt gcctctggcg agatgagcct gaaaaggctg caggagcagg acagcgaagg
      421 cgcggtggag atcatcgaga gcgtgaccgg ggcgggcacg cagcaggcat tggcggtcaa
      481 cctgcccaac cgcggttata tccaaaactt gcccgaaggc gccatcgtcg aagtgccagg
      541 ggttttgagc gacgacggag tgcagggcgt tgaggttggc tcgcttcccg cgccaattgc
      601 cgaattgtgc cggcgcgaga tcagcgccgc ccagctttgt gtagacgctg ccgtgcatgg
      661 cgaccgtcag gcagcgctgc aatgcctgct cctggacccg gtgatcaatg acatggacct
      721 cgccaagcag gtgctggacg actatttgag agcgtatcaa gacgtgctcc cccagttttg
      781 ggattgaaga aggcaagcag gacatgcccc gcatccccct gttgctgaaa tatatactcg
      841 tgctgtgctg cctacccata gccctgagcg gctgcgggct cagagagcta tatcaagaaa
      901 aaccggatga aggtaaaaat gcggcctccg ccggcaaaaa cgctggctcc agcgccaggc
      961 caaccgccac cctcaaaccc acagcaacca acacgcccgt gccgaccagc accttgggcg
     1021 tcgaagtcgc ggacttgcag ggcgtgtcca ttgtattctg gcatgcctgg acgggcgccc
     1081 cgggagatgt gatccaaagc ctggtggatg aattcaaccg cgacaaccaa tgggggatca
     1141 gcgtggaggc tgttgcgcaa ggcagttacg atcaggtttc cgagcaagtt agcgcggctc
     1201 tagagaacga ccagccgccg gatgtcacca ccgcctacgg ctaccaggcg cttggctgga
     1261 tttcacccgg tttatctctg atagatctag ccccttatgt ggacgacccg ctgtgggggc
     1321 tggatgaaca ggcgcagtct gattattacc ccatcttctg ggagcatgac ctgcagaatg
     1381 gggagcgcac cggcatcccc gcccagggct cagcacagct gctgtactac aacaacacct
     1441 gggccaagga gctggggttt gaaaatccgc ctgcaacgcc agaagagttc aaggcgcagg
     1501 cctgcgccgc aaaccaaacc tacaagcaag acagcgaccc caagaacgac cagaaagggg
     1561 gctggatcat ctccacggag tactctgcag ttttgggctg gatctattcc ttcggcggcg
     1621 agatagagag agcagatgga aatggctacc gcttcaacaa cgcgccggta aaggatgcct
     1681 tcacctacat gcgagagctc tatgagggca gctgctcctg gttgagcgag agccaattcc
     1741 cagaggatga ttttgccacc cgccagggct tgtttgctgc gggctcggtg gcgggcatcc
     1801 tttaccagga agaggcattc gtcgaagcga gcagcaagga tgagtggagt gtgatcgctt
     1861 ttccatccct aaatggcgag ccggtgatct cggcctacgg gccatccttt caggtgctcc
     1921 aatccacgcc cgaagagcaa ctggcgagct ggttgtttat ccgctggttg accgaagcgg
     1981 agccccaggc taaactggca gaggtgagtg gatactaccc ggtgcgcgcc tccagcgtgg
     2041 agatgatggg catcttgcct gccgcccacc cacaatggaa aagcgcagtg gacctgctcc
     2101 cctacgcgcg ccccgaaccc gcgctgggat cctggcgcat cgtccgttgg gcgctgagcg
     2161 acgcagccac ccagctattt cgcttctact ttgagagtga tcagctgcct gctctgataa
     2221 aacttctcga agagacttca aacgacttgc atgctggatt gaaatagatt aaggaggggt
     2281 ttatgaccta cgaatttatt ctcaccgaag tccacgaccg tgtggggctg gtgcgcctga
     2341 accgccccaa agccctcaat gcactgagta gcggcctgac ccgcgagctg atggatgcac
     2401 tattggcatt cgaggccgat gaagcgatcg gcgctatcgt tattacgggt gacgagcgcg
     2461 cctttgctgc aggagcagac attaaggaaa tgtccgaggc ctcagcggtc gagatgctga
     2521 aacgcgatat gatcggcaac ttcgacgcta tccagaaggt caccaaaccg gtcatcgctg
     2581 ccgtgtcggg ctggtgtctg ggcgggggca atgagctggc catgtcttgc gacatgatcg
     2641 tggcctccga aacggcgcgc ttcgggcagc ccgagatcaa cctgggtgtg atcccggggg
     2701 caggcggcac acaacgcctg acccgggctg tgggcaaagc gctcgccatg gagatggtgc
     2761 tgaacaaccg caccctaacc gcctcggagg ctttgcacta tgggctggtc aaccgcgtgg
     2821 tgccggtgga gcgctatctg caagaggccc ttgaattggc aaccgagatc gccgcccgcg
     2881 ccccacttgc agtgcgcatg ggcaaggagg cggtcaacca tgccttcgaa accaccttgg
     2941 ctgagggtat ggctgacgag cgccgcgcat tttacttcct tttcgccagt caagatcaga
     3001 aagaggggat ggccgcgttc acagaaaagc gcaaaccaca gtggaagggg atgtagccct
     3061 cactggctct gttcgccagg ctctgatgca gacacaggga tgatccggcg cgccagcgca
     3121 gcctagcctg agattttggc gcccctgtgc agcctagctg gtcaccgggc tgcagcgtca
     3181 tccacctgtt gacttttttg ataacctaag tacaataatt tcagggaata ttgccaaagt
     3241 tacgcagttg gagaggtcca tctctcttat aacagcgtaa gcctaaacgg ttcaatataa
     3301 gcagaggagg gtgccatgca aaccaaaaat atcgtcttaa ccacaattgc catccttttg
     3361 gcgttatccg tcatcgcggc cagcgttttg cccgctacga ccagcaccat caagaaaggc
     3421 gagacggtcg aattttatag cgggcgggca ggcgtcagct tcaccgatag ccagttcacc
     3481 ggggctgtac gcctggcccg cattgacgac agcaagatca aagccacaaa ccaccccaaa
     3541 ttcaagcacg atctgcttag cgtgcgtttt accaaatcca acggggagaa agtcacgcac
     3601 gtgctgggcg cggtgtacgt tttcttcatt gtaaaccgct ctgaggtaag cgcctgggag
     3661 aagggcgagc tggctgtgta cgtctacgat agctggaaga atgaatggca ggaatgctat
     3721 tcattcctgg tcaatgactc caacgggaaa tctagcctgg cctgccgaat gcgcattttc
     3781 agcacgtacg gtgtagccaa gaagtaatca gcagtctgct gatatttcct attctcccgt
     3841 tccgttcata tttgcgcggg aaataggcat ttttagcgtg gatagctcaa cccaaaaaac
     3901 tgtgccttgc cccaattggc tgtctacgcc gatcttaccg ccgtgcgcct caacaataga
     3961 tttcgcaatt ggcagaccaa gaccgcttcc cgtggatggg ccatagcgag gtcgcgtagc
     4021 ctcaacgcgg taaaaacgct cgaaaaggtg ggggagatgc accggtgcaa tcccataacc
     4081 tgtatcgctg actttcagta gaagatttcc tccttgctca tttacttcca aggaaatctc
     4141 tacccgccca ttgcgagcag tatacttcac ggcattgtcc accaggttat acagcatacg
     4201 ccgcaactgc tcctcgttgc ccatcacggg aggcagatct ggatggatgg aagatcctag
     4261 ctgaatctcg gctcgctcag cccgcacctt gaaggcatca aagacatcgt aggtgatagt
     4321 agccaaatca actggctcat gccggctcgg gtccagcccg gtttcgattc gcgacagatc
     4381 caatagatcg ttgaccatac tgctcatgcg atccacctcg ctttcgactt cacctagaaa
     4441 gcgttcagat accgggggat catccaaagc accgttgcgc agagcttcga cacgcaactt
     4501 aattgaggtc agcggtgtgc gcaactcatg gctggcgttc gccacgaagc tgcgcaactc
     4561 gttcacgtag gactcaagcc gggccgccat attattgaat gccaccgcca aaaggttaat
     4621 ttcctgtaga ttggtcgatg agtcaaagcg cgcgctcatc ttatcgctgg tcaggctctc
     4681 ggcggcatgc gtgatttgct caatcggctc tgccagactg cgcgccagga aaaagccagc
     4741 tacactcacc accagcccaa tgatcaaagc aaacccaatc agcaataaca aggaggagcg
     4801 cgctggtttc aggacggctt ccaagggaat attgatgctt aatagcccat acacttgatc
     4861 ctcgcgctgg atgcgcgtga cgagataaag gaactcttcg ccttgccatg accgacgaaa
     4921 tacttcccct tcccctgttt gcctagataa ggcttcccaa agctcaggcc tggtttgcgg
     4981 atcagcggca gggggtaaat tgcctgaact atccatcaga gggattccat caggcaggta
     5041 aatcgtgtaa tggacttccg gttggctgga aaaaatccgg gctagcgatt ggcgaactcg
     5101 ttcatttgac gactggccag acagataatc gatcaaagac ccctcgacat ctctggcagc
     5161 tgcaaaactc agaacttcat aacggtgttc ggcttgctgg cggacggcag agtatagaat
     5221 ttggccagca taagaagcga tcagggccac gctggcaagt gaaaccagca gatgggaaag
     5281 gatcaaccgc gcctggatcg tatgaggcag aaaaaagcgc atttattcca attcttctgg
     5341 cccagcaaat cgatagccgt gaccacgcac ggtctgtata tatttagggc tggcaggatc
     5401 atcttcgact tttaagcgca gccaacgcac atgcacatcc agggtgcgtt ggtcgccaat
     5461 ccagtcactc ccccacacct ctccaagcaa atcctcgcgg ctcagggcac gacccgcatt
     5521 cttcatcaag caagaaagca aatcaaactc gcgcgcggat agctgcactt cctggccgct
     5581 cttaaacact cgccgcgccg tagaatctag ggtcaggtta cctaagttaa tcgattgcat
     5641 cattgatacc tggcgatcca gctccacacg gcgcaaaatc gagcgcacgc gagccaggag
     5701 ctcccggaat gagaacggct tcgccagata atcgtctgcc cccacttcca gacccaatac
     5761 gcggtcgatc tcctcgccac gcgcggtgag cataatcaca gggatggtac tttgggcacg
     5821 caatcggcgg cagatttcta ggccgttcat gccaggcagc atcacatcca gcaccaccag
     5881 atccggcgca taatccagga tttcctgtag agctatggag ccatctccca ctgatttgac
     5941 atcaaacccc tcgcggcgca agctataggt gagggaatcg gtaatgagag gttcatcgtc
     6001 aacaagcata atgcgcgtca aggtccacct ccaaatccag tttttattcg aaactctcga
     6061 tcgcacacaa agataatcaa tgttttaaga aaatacgatt aagttctgtt taaggttttc
     6121 caaatagccg tcacgctttc gcccaaatgg cgacaatata ggttaggaaa gtcggcgatt
     6181 gggcaattta gctttcaata ttcaaggcta aattatgcaa atcttatcac attctgtgag
     6241 aaaaaccggc aaattacctc atgaggagag tgcacccgtt ttcatccatc tttggattcc
     6301 tggccactgc tcttttattg gccggtctgg ctatgcttct tgtgcgggac ggagggattg
     6361 ccttcagtcc gggcaaattg agtgcgaaaa gccagcccgg cgtttctctg cagggtttta
     6421 cttcacacgc cgaaatcgag ccggattgca gccgttgtca ccagccgctc aagaccacgc
     6481 aggacactct gtgcctagag tgccacagca gcgtggccgg gcaaatccat cgcacggatg
     6541 gggcgcacag cttgatagct gacatcaggc gttgttttgc ttgccatacc gatcaccaag
     6601 gagaagattt cgaccctaca caagcggcat ttgccttgtt cgatcactcg aaaacgcggt
     6661 tcagcctgct tcgtcaccag gtcaattatg acgcatcgcc aatggagtgc ctcgcctgtc
     6721 accagtatga agggaagatg gcgttttcgg attcatattg cactttgtgc cacgctgcgg
     6781 aaaatatgga ctttgccgtc cagcacagcc agcaatacgg tgcaaactgc tcatattgcc
     6841 atgatggagg agaccgcctg gctgacttcg accaccaaac gactgctttc cccctggaag
     6901 gaaaacatgc acaggcttac tgtgccgatt gccacgcgcc gcaggaactc gctgttgggc
     6961 gcggcggtgg aggcttatcc tcaagtcagc ctgtgggcgc gccaagtgat tgcgtcg
//
LOCUS       DBNHOH010000631         1241 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_422136, whole genome shotgun sequence.
ACCESSION   DBNHOH010000631 DBNHOH010000000
VERSION     DBNHOH010000631.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_422136"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1241)
                     /locus_tag="AC16V7_11050"
     CDS             complement(<1..>1241)
                     /locus_tag="AC16V7_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR6257701.1"
                     /translation="LTPSETPTPGQEIPTPVSATPIPESEVTFRVEIPANSPGDQPVY
                     LNILDEVSGLALNMQTYPMDQEDPLHYSLVLPLPYASAVKYRYSRQGPAAMVEEHVSD
                     GRQMRYRLYHVEGPGVVQDVVTRWTDTKYEGSTGRINGQALDSQNGQPITNLLVVAGG
                     SQALTTSDGSYLLEGLPPGTHNLVAYSLDGTYRTFQQGAVIAPESTTPAELRLTQAPV
                     VNVTFEVSVPAGTIPAVPIRIAGNLVQLGNTFGDLAGGVNTVASRMPVLNLLPDGRYN
                     IQISLPAGADVRYTYTLGDGYWNTELNQDGSRRVRQLIVPESDVIIQDTVDSWLAPDS
                     APITFDVNVPANTPVDDFVSIQFRPLYGWTEPIPMWHLGNNRWAFVLNNPLQTIGSMS
                     YRYCRDDQCGAADDTQTMGPA"
BASE COUNT          278 a          318 c          348 g          297 t
ORIGIN      
        1 ttgccgggcc cattgtttgg gtatcatcag ctgccccgca ctggtcgtcc cggcagtagc
       61 ggtaactcat actgcctatc gtttgcaacg ggttgtttag aacgaaagcc caacgattgt
      121 tcccaaggtg ccacattgga ataggttctg tccagccata aagaggcctg aactgtatcg
      181 aaacaaagtc atccactgga gtgtttgctg ggacgttcac atcgaaggta attggtgcag
      241 aatcgggggc taaccatgaa tccactgtat cctggataat gacatcactt tctgggacaa
      301 tgagctgccg gacacgtcgg ctgccatcct ggttaagttc agtattccaa tagccatctc
      361 caagcgtgta ggtgtaacgc acatctgcgc ctgcgggtag agatatttgt atgttgtatc
      421 gcccatcagg cagaagattt agcactggca tacgtgatgc caccgtgtta actcctcccg
      481 ctagatcacc gaatgtattt cccaattgga ccaggttgcc agcgatgcgg atgggtacag
      541 ctgggatggt gccggcgggt acgctcacct caaaggtgac attcacaacc ggggcttgcg
      601 ttaagcgcag ttccgcaggt gtcgtagatt caggcgcgat aaccgcccct tgttggaaag
      661 tgcgataagt cccgtcgaga gagtaggcaa ccaggttgtg cgtacccggc ggcaatccct
      721 caagcaagta tgagccatcc gaagtggtaa gcgcttgtga gccgccagcg acaaccagca
      781 ggttggtaat tggttgaccg ttttgtgaat caagagcctg cccattgatg cgcccagtgg
      841 agccttcgta ttttgtgtct gtccagcggg ttaccacatc ctgcactacg cctgggccct
      901 caacgtgata cagcctgtaa cgcatctggc gaccatctga gacatgctcc tctaccatgg
      961 ctgcgggtcc ttgacgcgaa taacggtact tcactgcgga ggcatagggt aacggcaaaa
     1021 ccaaggagta atgaagtgga tcttcttgat ccatcggata tgtctgcatg tttagagcca
     1081 gcccgctcac ttcgtctaaa atattcaagt aaaccggttg gtctccgggc gagtttgccg
     1141 ggatctctac gcggaaagta acctcgctct ccgggatggg ggttgcgcta acgggagtag
     1201 gaatctcttg cccaggtgtg ggcgtctctg agggcgtcag g
//
LOCUS       DBNHOH010000632         8051 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1576007, whole genome shotgun sequence.
ACCESSION   DBNHOH010000632 DBNHOH010000000
VERSION     DBNHOH010000632.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8051)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8051)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8051
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1576007"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1193
                     /locus_tag="AC16V7_11055"
     CDS             117..1193
                     /locus_tag="AC16V7_11055"
                     /inference="COORDINATES: protein motif:HMM:NF048802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7544 domain-containing protein"
                     /protein_id="HIR6257702.1"
                     /translation="MDHMQTLKRAWNILWSYRALWVIGIILALTSPSGFSPGSSNFSP
                     DLNVPGRYETTPPEEIQRELEKLGELFNQGIPADTLETLTRIGVLFVLAMLLLAILFA
                     IGYYVSQAALIRMVDKYEQDEEKLTWKAGLRLGWSRAAWRLFLIDLVIYLPIFLAFIL
                     IFGCAALPVLLKTLAGQQPTMPGIIASVGLVFLLIFVAIIINVILSLVLEIIRRVGVL
                     QGSDAITSIRQGLEVIRGNFKDVFIMWLILLGIKVGFFIALIPVAILLAALGLFVGGG
                     VGFLFYTVFQATTSTLVAWIAAVLSGGILFILVLAIPMLFLEGLRETYLSSTWTLTYR
                     ELNLEMLDRSSSPEESNPSAEVVA"
     gene            1555..3000
                     /locus_tag="AC16V7_11060"
     CDS             1555..3000
                     /locus_tag="AC16V7_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HIR6257703.1"
                     /translation="MNIQLSNGNVIPVEMHKVRIIQKTSLPPIDRRLAAIQEAGYNTF
                     LLRTRDIFLDMLTDSGTNAMSDNQLAAMMVSDDAYAGCESFYKLADAVKDVLGFNYVI
                     PVHQGRAAEHLLAKAFVKPGDVVLMNYHFTTTKAHFELAGASVLELYTAEALNTQSSE
                     PFRGNLDLNRLKDAIQEHGADRVSFIRMEATTNLIGGQPFSLCNLRAVKEIAEANNIP
                     LVFDGSLISENAYFIKMRECEYAAQSITKLIQEMMKMVDIFYMSGRKSTSVRGGMMAT
                     NHKEYYDRILAWLPVYEGFATYGGMSTKEIEAMAVGLREMTDVNVAGSSPEFIQYFVN
                     RLVENGVPAVTPAGGLAGHVDASRFLPHIPQSQYPAGALAAAIYLASGVRSMERGTIS
                     MDRDLDGNEVYADLELARLALPRRVYTLSHIEYVVDRLTWLYEHRHLVKGLRFVEEPK
                     VLRFFFGKLEPLDHWGYQLAEAFKAEFGENF"
     gene            3248..4084
                     /locus_tag="AC16V7_11065"
     CDS             3248..4084
                     /locus_tag="AC16V7_11065"
                     /inference="COORDINATES: protein motif:HMM:NF016668.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HIR6257704.1"
                     /translation="MKASEVLGYPKDARLLIVNIDDYGLCFTVNDTAIQIINKGAASS
                     CTVMTPAPWGIHGIDLVKANPGVKCGVHLTAISEHVCYRWKPLSSPETVPSLIDSDGY
                     FFLETYQDQFTRQADVHQLEREWRTQIEFALRRGLQVSHLDSHCNVHDSRDDFFAMTY
                     SLAQEYGFPLRIHNEKYLHQVKKEDRVTIDYPDLDSFRVPLENKSLLYVQMLRELPEG
                     LSEWAIHPAYDSAELRAISPEWPVRVTDYEFFSSDEFLHVIQTEGIELIDYSMLKPYW
                     VP"
     gene            4149..5351
                     /locus_tag="AC16V7_11070"
     CDS             4149..5351
                     /locus_tag="AC16V7_11070"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011605756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR6257705.1"
                     /translation="MPGPGYYFFGEEERKEVEDVLTSGYLSRYGREDDPLFKQKVFTF
                     ESEFARKMGARYCVAVNSGTSALMAALVAIGLQPGDEVLVPGYTFIASMSSVIAVGGH
                     PVLVEVDESLTMDPVDVERKITERTRVIMPVHMLGNPCDMERILTLAEKYNLIVIEDC
                     CQALGGSYCGQRLGTYGAFGGFSLNNYKVINSGDGGVLITNNQELYKRAFAFHDQGHL
                     PLRKGVEIGQRTLMGVNMRMNELTGAFALGQLRKLDLILSTLRKKKALFKQNIQAGDI
                     RNMQFRRINDVEECATLLTVQFPDEALTEKVVKALQTKSVYNSGWHVYNHMEQFLSYT
                     DASGKHPYHKNMLPQTDEILRRSVNLSVGVVDPGLGADFGINVLSSDDEIQQKAEEFI
                     RIVKPIAG"
     gene            5513..6511
                     /locus_tag="AC16V7_11075"
     CDS             5513..6511
                     /locus_tag="AC16V7_11075"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6257706.1"
                     /translation="MSAFADKIAVMRYRLSYIPEAVALISFIALFLFFGFAAPNFLTP
                     LSIGNILTFGSITAIVTIGVAILMVAGEFDLSVGSNFAVTSYVIALSLIAGLSVWIAI
                     PLGILVSTLLGMLNGLIVTKTRIPSFICTLGTMLAYRGIVRAIGKGSFATYKGEPIPL
                     FSVLNGDFDLLNNVFGMQAFRTSMVWFILLAIIFSFVLMRTRFGNWIFAGGGAADAAR
                     AQGVDVTRVKILCFTLTGFLVALASTLQFAHRLSVDPLRGDGMELIAVASCVIGGVRL
                     TGGYGTVFGAILGTFLLQMLDQGLVLMQIPIQVFQACVGLIIIISVILNTYLGKSD"
     gene            6657..>8051
                     /locus_tag="AC16V7_11080"
     CDS             6657..>8051
                     /locus_tag="AC16V7_11080"
                     /inference="COORDINATES: protein motif:HMM:NF024799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR6257707.1"
                     /translation="MKKLNILLVLLVVFTLLLTACQPQPTPAPAEPTQPPAAEQPPAE
                     QPTEAPPAEQPTQPPAPEATEPPAPPPAEGALNLIMVQHALCAWDSFWCTVEDGIKTA
                     AAQMGVEVTILGPDKFDLEKTASLIDQAVAAQPDGIGLTVTDPVLFKEPIMKALDAGI
                     PVLAYNAGKGPLEDGIPYMTYLGMDEYQGGYQSGQRMAAAGASSGVCINHQVGHAGLD
                     ARCKGFVDAFTEKDLPAEVLAIKGEDAAQSQTTISDFYAANPDVDAFLTLGPSGANPF
                     YAFLEAEQPEGVMHGTFDLSPEIEANIQDGTTMFAVDQQPFLQGYNAVVYLVLANKYG
                     IRPALPVTATGPGFIDKNLVGKATQPDRPVKLIMVQHALCAYDPYWCVVEIGMQEAAE
                     HMGAELTILGPDKFDVEKTAALIDQAVASAPDGMGVVITDNQMFKPPLMKAVDAGIPL
                     VAYDTGLGPEADGIP"
BASE COUNT         1887 a         1956 c         2172 g         2036 t
ORIGIN      
        1 cggaattggc gcaggtctgc tgatcagcct gatgtatgtc tgctggttag tgggctggtg
       61 ggtttggaaa cagcgaacag cagtaagcca agtttgattt gaaaggagta cgaaaaatgg
      121 atcacatgca aactctcaag cgagcctgga atattctatg gagctaccgg gcactctggg
      181 tcatagggat tatattggca ttgacttcgc catcaggttt ttccccgggg agcagtaact
      241 tctcacccga tttaaatgtt cctggtcgct atgagacaac cccaccagag gagatccagc
      301 gcgagctgga gaagctgggt gagttgttca atcaaggcat tccagctgat accctggaaa
      361 cgttgacccg cattggcgtg ttgtttgtgt tggctatgct cctcttagcc atcctgtttg
      421 ccatcggata ttatgtcagc caggcggcgt tgatccgcat ggtggataaa tacgagcagg
      481 atgaggagaa attgacttgg aaggcaggtc tgcggctggg ttggtcacgg gcagcctggc
      541 ggctgtttct gatcgatttg gtgatttacc tgcccatttt tttggcgttc atcctgattt
      601 ttggttgcgc agccttgcct gtgctattaa aaaccctggc aggtcaacaa cccacaatgc
      661 ccgggattat tgcctcggtg ggtttggttt ttctgctcat ctttgtggca atcatcatta
      721 acgtgatttt atcgctggtg ctggagatca tccggcgagt gggcgtcttg cagggtagcg
      781 atgccattac ttcaatccgc caggggttgg aggtaatacg cggcaacttc aaggatgtgt
      841 tcatcatgtg gctgatcttg ctgggtatta aggttggctt cttcatcgcc ttaatacctg
      901 tggctatcct cttggctgca ttgggtctat ttgtgggagg aggcgtaggc tttctctttt
      961 acacggtttt ccaggcgaca actagcacct tggtcgcttg gatcgcggct gttctatccg
     1021 gagggatcct gttcatcctc gtgctggcga ttccgatgct cttcttggag ggcttgcggg
     1081 agacatatct gtcatcgaca tggacattga cctatcgcga gctcaacctc gagatgctag
     1141 atcgaagcag ctcgcctgag gaaagcaacc catccgcgga ggtggttgcc taagggcaga
     1201 agcgtaaggg taaggtataa agggttgagc ggatacttat ttaacatccg ctcaaccctt
     1261 ttttgcttga ttttgattga caattatgct attctgtaaa ttaaatgtga cataaatgtg
     1321 agcaagtaat tgtatgctca tcacaagttt gatcttggct tatcccgtag agggacaaat
     1381 tcttaggaag tgtgtggggg tatggtgtct taaaagagga gggaaaatcc gatttttatt
     1441 tgcctgttgc gctttgttta ataccatacg agctagggat gaaacttggt tttgaattca
     1501 tcacctggcg ctaaagcgta ttctgtgtga tacccacaga aggagataag acaaatgaat
     1561 atccaacttt cgaatggtaa cgtaatccca gtggagatgc acaaagtgcg tatcattcaa
     1621 aagacgagtt tgccgcccat tgatcggcgg cttgcggcaa tacaagaggc gggttataac
     1681 accttcttgt tacgcacaag ggacatcttc ctggatatgc ttaccgatag cggtacgaat
     1741 gcaatgagcg ataaccagct agctgccatg atggtgtcgg atgacgccta tgctggttgc
     1801 gaaagcttct ataagctggc agatgccgta aaagatgtct tgggcttcaa ctatgtaatc
     1861 ccagttcacc aggggcgagc agcggaacac ctgttggcga aagcctttgt caagccgggt
     1921 gacgtggtgc tgatgaatta tcactttacc acgaccaagg cgcattttga gctggctggg
     1981 gcaagtgtgc tcgaactata tacggccgaa gcgctgaaca cccagagcag cgagccgttc
     2041 aggggtaacc tggatttgaa caggctgaag gatgctatcc aggagcatgg cgcggatagg
     2101 gtgtctttta tccgcatgga ggcaacgacc aatctgatcg gcgggcagcc tttctcgttg
     2161 tgcaacctgc gagcggtcaa agaaattgcc gaggcaaata acatccccct ggtgttcgat
     2221 ggcagtctga tctccgagaa cgcctatttc atcaagatgc gcgaatgcga gtacgccgct
     2281 caatccataa caaagctgat ccaagagatg atgaaaatgg tggacatctt ctatatgtct
     2341 ggacgcaaaa gcacctcggt gcgcggcggt atgatggcca ccaaccataa agaatactac
     2401 gaccgcatct tggcctggct gccggtttac gagggatttg ctacctatgg tgggatgtcc
     2461 accaaggaga tcgaagccat ggcagtcggc ctgcgtgaga tgacagatgt taatgtggca
     2521 ggtagctcgc ccgaattcat ccagtatttt gtcaatagat tggttgagaa cggcgtccct
     2581 gcagtgacgc cggctggggg attggcggga catgtggatg caagtcgctt tttaccccac
     2641 atcccgcagt cgcaataccc ggccggggca ctggcggcag ccatttatct cgcttcgggg
     2701 gtgcgcagca tggagcgcgg cacaatctct atggaccgtg atctggacgg taacgaggtc
     2761 tacgcagact tggaattggc acggctggca ctgccaagac gggtgtacac gctctcccat
     2821 atcgaatatg tagtggaccg cctgacctgg ttatatgagc accgtcatct ggtcaagggg
     2881 cttcgcttcg tggaggaacc caaggtgttg cgtttcttct ttggcaaact ggagccattg
     2941 gatcattggg gatatcaact ggcagaggca ttcaaggctg agtttgggga gaatttctaa
     3001 aaccagacgt gaataaagac gaggttgttc gctggtaatc gcgaataatc catcgtttcg
     3061 gaaaatatgg ctctccaaca ttgtgctgtt ggagagccat atcattgata gagattaatt
     3121 ttgcagtcat aagttccgaa ttcaaaagtg tagtaaaata gcttatgcac acgtgtgcat
     3181 aaagtaaagg gcagtctcct tatccagtat tgatccagaa tatctcaagt tatccatggg
     3241 aattcccgtg aaggcaagtg aggtccttgg ttatccaaaa gatgctcgtc tgctgatcgt
     3301 caatattgac gattatggct tatgctttac ggtcaatgac actgccatcc agataatcaa
     3361 caagggggca gctagttcct gcacggtcat gacacctgcc ccgtggggga ttcatggaat
     3421 tgatctggtc aaagcaaatc ctggggtgaa atgtggcgtc catctaactg ccatcagcga
     3481 gcacgtatgc taccgttgga aaccgttgtc ttctccagag actgtcccct cattgataga
     3541 ctccgacggt tatttcttcc ttgagacgta ccaggatcaa ttcacccgtc aagccgatgt
     3601 ccaccaattg gaacgcgaat ggcggactca gatcgaattt gcgctgcgcc gcggccttca
     3661 ggtttctcac ctggacagcc actgcaatgt ccacgattcg agagacgatt tctttgcgat
     3721 gacttactcc ctggcgcagg aatatggatt tcctctgcga attcataatg agaaatattt
     3781 acatcaagtg aagaaagaag acagggtgac gattgattat cccgatctgg atagcttcag
     3841 ggtgcccttg gagaacaaat ccctgctcta tgtccaaatg ctgagagaac tcccggaggg
     3901 attgtctgaa tgggcaatcc acccggccta tgacagcgcc gaactgcgcg ctatcagccc
     3961 cgaatggccg gtacgggtaa ccgattatga attcttcagc tccgatgaat ttctacatgt
     4021 catccaaacc gaagggatcg agcttattga ttattccatg ctaaaacctt actgggtgcc
     4081 ttaaaagtcg ggcttcgaaa gcccggaatt ttccggaacc ctctaactgc tattctcagg
     4141 aggcatacat gccaggtcca ggttattact tttttggaga agaggaacgc aaagaagtag
     4201 aagacgtact caccagcggt tacctgtcgc gttatggcag ggaagatgat ccgctgttca
     4261 agcaaaaggt atttacgttt gaatcggagt ttgcccgaaa gatgggagct cgctattgcg
     4321 tggcggtaaa ctcaggtacc agcgcgctga tggccgccct ggtggcaatc ggacttcaac
     4381 ccggagatga agtgctggtg cctggttata cctttatcgc ctcgatgtcg tctgttatcg
     4441 ctgtcggcgg gcaccctgta ctggtagagg tggatgaatc cctgaccatg gatccagtag
     4501 acgttgagcg aaagatcacc gagcgcaccc gtgtgatcat gccagtgcat atgttgggca
     4561 acccctgcga tatggagcgc atcctgaccc tggcagaaaa atacaacttg atcgtcattg
     4621 aggattgctg ccaggcgctg ggtggctcat actgcgggca gcggttgggc acgtatgggg
     4681 catttggggg cttctcgttg aataattata aggtcatcaa ctctggtgat gggggagtgc
     4741 tgatcaccaa taaccaggag ctttacaagc gcgcttttgc tttccatgat cagggacatc
     4801 ttcccctgcg caaaggcgtg gagatcggtc aacgcaccct gatgggggtc aatatgcgca
     4861 tgaacgagtt aactggcgcc tttgctttgg gacagttaag aaagttggac ttgattctct
     4921 ccaccttgcg aaaaaagaag gctttgttca aacagaacat acaggcaggc gatattcgca
     4981 atatgcagtt ccgtcgcatt aacgatgtag aagaatgcgc caccctgttg accgtccaat
     5041 tccccgatga agccttgacc gaaaaggtgg ttaaagcttt acaaaccaaa tctgtctaca
     5101 atagcggttg gcatgtctac aatcacatgg agcaattcct gtcttacacc gacgcgagcg
     5161 gcaaacaccc ctaccacaag aatatgctgc cgcagaccga tgagatcctt cgtagaagcg
     5221 taaatctcag cgtaggcgtg gtggaccctg gcctaggcgc agactttggg atcaacgttt
     5281 tgagcagcga tgatgaaatt caacaaaaag ccgaagaatt catccgcatc gttaagccaa
     5341 ttgcaggcta ggatcagcgt taatatttat gattctcacc tatccggtga tgcgagaatt
     5401 gacaagcaaa ataatgtatg ctagactctt tgcaaacatt tgcataaagt ggatttggca
     5461 atacagaggg gatatgtaat gaaagaatgc agagcactct tcgaggtgaa gaatgagcgc
     5521 gttcgctgac aaaattgcag tgatgagata ccggttatct tatatccccg aagctgtcgc
     5581 gctgatcagt tttattgccc tgtttctatt cttcggtttc gccgccccca attttctaac
     5641 ccctttatcc attgggaata tcctgacctt tggtagcatc acagctatag tcaccattgg
     5701 cgtggcgata ttgatggtcg caggtgagtt tgatctctcc gtgggctcga atttcgcggt
     5761 caccagctat gtcatcgccc tgagtttgat tgctggtcta tctgtttgga tcgccattcc
     5821 ccttgggatt cttgtgagca ccttgcttgg gatgttaaat ggattgattg tcaccaagac
     5881 gagaatccca tcattcatct gcactttagg cactatgctg gcataccggg ggatcgtgcg
     5941 tgcaatcgga aagggtagct tcgccaccta caaaggcgag ccgatcccgt tattcagtgt
     6001 cttaaatggc gatttcgacc tgctcaataa tgtcttcggc atgcaggctt ttcgcacttc
     6061 catggtctgg tttattctgc tagccatcat tttttcgttt gttttgatgc gtacgcggtt
     6121 tggaaattgg atttttgccg ggggtggagc ggcagatgct gctcgggcgc aaggggtgga
     6181 tgtgaccagg gtgaaaatcc tgtgttttac gctgacgggt ttcttggttg ccctcgccag
     6241 caccttgcag tttgctcacc gtttgagtgt agacccgctg cgcggtgatg ggatggagct
     6301 tattgcggtt gcatcctgcg tgattggagg cgtgcgcctg accggtggat acggaaccgt
     6361 gtttggcgca atcttgggta ctttcttact ccagatgtta gatcagggcc ttgtcctgat
     6421 gcaaatccct atacaggtgt ttcaggcttg tgttggcctg atcatcatca tctcagtaat
     6481 tctcaacacc tatttgggaa agagcgatta aagcctctct tcactattgg atgcaaaact
     6541 ctttctttgg atcataagga ggtgatgacc tgataacgcc atatgtgaaa aatattccaa
     6601 gctgtttatt ctcagtgtaa gacaactctc tcaagatttg taaatatgga ggaagaatga
     6661 aaaagctaaa tattttgctg gttttacttg tggtgtttac attgttgctt acagcctgcc
     6721 agccacaacc cacgccagcg cccgcagaac caacccagcc acccgctgcc gagcagccgc
     6781 ctgctgagca gcctaccgag gcgccacctg cggagcagcc cacgcagccg cctgcaccgg
     6841 aggcgaccga gccgcctgct ccaccaccag ccgaaggagc cctcaacctc atcatggtgc
     6901 agcacgccct gtgcgcatgg gactccttct ggtgcacggt tgaagatggc atcaagacgg
     6961 ctgccgctca gatgggtgtc gaagtcacca tcctcggccc agataagttc gatctggaga
     7021 agaccgcttc cttgatcgac caggctgtgg ctgcccaacc ggatggcatc ggcctgaccg
     7081 tcaccgaccc ggtgttgttc aaggaaccga ttatgaaggc cctcgatgct ggcatcccgg
     7141 ttttggcata taacgctggc aaaggcccgc tagaagacgg catcccctat atgacgtatc
     7201 tgggcatgga tgagtaccag ggcggatacc agagcggtca gcgcatggcc gcggctggcg
     7261 cttccagcgg agtttgcatc aaccatcagg tcggtcatgc cggtttggat gcccgctgca
     7321 agggcttcgt ggacgccttc actgagaagg atcttcctgc cgaggtcctc gccatcaaag
     7381 gtgaagacgc tgcccagtca cagaccacca tctccgattt ctacgctgcc aacccggatg
     7441 tggatgcgtt cctaacgcta gggcctagcg gcgccaatcc cttctacgct ttcctagaag
     7501 ctgaacaacc cgaaggcgtc atgcatggca cctttgatct cagccccgag atcgaagcca
     7561 acatccaaga tggcacaacc atgttcgctg tcgaccagca acctttcctg caaggctaca
     7621 acgcggttgt ctatctggtg ctggctaata agtacggcat ccgaccggcg ctgcctgtca
     7681 ccgccaccgg tccgggtttc atcgacaaga acctggttgg caaggcgacc cagccggatc
     7741 gacccgtcaa gctcatcatg gttcagcacg ccctatgcgc ttacgaccca tactggtgcg
     7801 tagttgagat aggcatgcaa gaggctgccg agcatatggg cgcagagctc actatcctcg
     7861 gccccgataa atttgatgtt gagaagaccg ccgcgctcat cgaccaggct gtggcctcgg
     7921 ctccagatgg catgggggtg gtgatcacgg acaaccagat gttcaaaccg cccctcatga
     7981 aagctgtgga tgctggtatc ccgctggtcg cttacgacac gggcctgggt ccggaagcgg
     8041 atgggatccc c
//
LOCUS       DBNHOH010000633         2872 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1319967, whole genome shotgun sequence.
ACCESSION   DBNHOH010000633 DBNHOH010000000
VERSION     DBNHOH010000633.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1319967"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            439..1029
                     /locus_tag="AC16V7_11085"
     CDS             439..1029
                     /locus_tag="AC16V7_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257708.1"
                     /translation="MSSSNLVRWSGMATIVGAILIAILELMEFILFGSLTESQVAATG
                     GWVFVQVIYLIAMLALLLGLIGLYTRQVEQSGNFGVISFLVAFIGTVLFAGAQWSTAF
                     IGPMLAAEAPGIMDAEPTGSILVGYLLSIILFSLGWLLFGWASLRSSVFPRGAALLVA
                     VGGVLFFIMGSLEWPAATLALDAGLVWMGYTLWSAK"
     gene            complement(1154..1227)
                     /locus_tag="AC16V7_11090"
     tRNA            complement(1154..1227)
                     /locus_tag="AC16V7_11090"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1191..1193),aa:Asp,seq:gtc)
     gene            complement(1282..1353)
                     /locus_tag="AC16V7_11095"
     tRNA            complement(1282..1353)
                     /locus_tag="AC16V7_11095"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1319..1321),aa:Met,seq:cat)
     gene            complement(1379..2833)
                     /locus_tag="AC16V7_11100"
     CDS             complement(1379..2833)
                     /locus_tag="AC16V7_11100"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR6257709.1"
                     /translation="MQERRDSRTLVMKFGGTSVGSEDAFRQVCEIVEQARSEWPRMVV
                     VTSAMAGVTNLLLESASKAVEGSTEYVHQIAQRIRTQHWEILAALLPDLARQAQVKQE
                     INHLIGEFTNLCQAIQVLGEATPRALDAVASLGERLSVRVLAAALDSRGMPAQFVEAT
                     QLIITDNQFQSAHPDFELTRQAIGRVLEPILGQGKIPVITGFIAATPDGITITLGRGG
                     SDYTAAILGAHLPADDVWIWTDVDGVMTADPRAVPGAHTISQLSYREIAELAYFGAKV
                     VHPKTIRPIIEAGIGLRVCNTFNPNHPGTRVVADHPLSQKNNSISNETIKAVTTIRGQ
                     SLITVEGRGMLGVPGVAARTFGAVAATGTSVPLITQASSEQSICFAVPIDTVDSVIAS
                     LESAFANELNRKDIDRIWATEEVTIITIVGSGMRNTPGIAGQIFSALGDRGVNVIAIA
                     QGSSEVSISLVVDAKDADAGVRSVHDLIDRIIRP"
BASE COUNT          685 a          749 c          687 g          751 t
ORIGIN      
        1 atgagaggtg ctaactaatg cgctatgttg taaaacaact aggcgagccc cttctaagac
       61 tggcacaaac ccaatttcat cccccaataa aatgcgaggg tattttctct tggcggtatc
      121 tgcttcaaga tttatatagc tggctgattt cacctgcttt ctgcactgcg gtggcatgac
      181 cctttgtgat caatcacaaa gggtcattta ttactctttc ccaagttaaa gcttgacgca
      241 ctttcgtgga ttacacaaaa tgatagtcgt cggcagaaag gaagctgtta aacaagcgaa
      301 taatcacccc ttctaactaa atacgaatac actgcaagcc ttggttggcg atgcgaaaac
      361 gaattgtagg ttatcgtttt gcgctatgta ctgggtcaga agaactaaat tcaggatatc
      421 acaaaggagg aaattaaaat gtcgtcttca aatctcgtgc gttggagtgg catggccaca
      481 attgtgggtg ccatactaat tgccatcctc gaattgatgg aatttatcct gtttgggagt
      541 ctaacagaaa gtcaggtggc ggcaacaggt ggctgggttt tcgtacaagt gatatatctc
      601 attgctatgt tggccttgct actgggttta attggtctgt atacacgcca ggtagaacag
      661 tcagggaatt ttggggtaat ctccttcttg gtggcattta tcggcactgt gctgtttgct
      721 ggcgcgcaat ggagcacagc ctttatagga ccgatgttgg ctgctgaagc tcctggtatc
      781 atggacgccg agcccaccgg gtccatactt gtgggatatt tgctttccat aatattgttt
      841 tcgcttggct ggcttctatt tggctgggcc tcgctgcgat ccagcgtttt tccgcgcggc
      901 gcagcgctgc tggttgcagt tggaggtgtg ctattcttta tcatgggatc acttgaatgg
      961 cccgctgcga ctttagcctt ggacgcaggc ctggtctgga tggggtatac gctctggtcc
     1021 gcaaagtaaa acagttgtaa gggattaagc ttttctcttc gcttacatac tgacgatgat
     1081 cttttcgtca gggatgtgtg aataggatat agataacgaa atcgcatagt gaaaactatg
     1141 cgatttcatt ctgcggtccc gacgggattc gaacccgcga tctcggcctt gacagggccg
     1201 catgttaggc cgctacacca cgggaccagg caagccgtag tttaatcgat ttcaaaccct
     1261 ccgtcaagcg ctacggctca gtggcgaggg gcggatttga accgccaacc aagggcttat
     1321 gagtcccctg ctctgccatt gagctacctc gccagtcatt gaggcgggct aaatattact
     1381 acggtctaat tattctgtca atcagatcat gcaccgagcg cacacccgca tcagcgtcct
     1441 ttgcatccac taccaggctg atagatactt ccgacgaccc ttgggcaata gcgatcacat
     1501 tgacgccacg gtcacctaat gcgctgaaga tttgtcccgc tattccgggc gtattgcgca
     1561 tccccgagcc cactatcgtg atgatggtga cttcctcagt cgcccaaatt cggtcgatat
     1621 cctttctatt caactcattc gcaaaggctg attccagaga agcaatcaca ctatcgactg
     1681 tatcaatcgg cactgcaaag caaatcgatt gctctgaaga tgcctgagta atcaacggaa
     1741 cgctagtacc tgttgcagcc acagctccaa atgtgcgcgc tgcgacacct ggaacaccta
     1801 acatccccct cccctccaca gtgatcaggc tttgcccgcg tatggtggta actgctttga
     1861 tcgtttcatt cgaaatgctg ttgttctttt gactcaaagg atgatcggct acaactcgtg
     1921 tgccaggatg atttgggttg aaggtattac aaacacgcaa cccaatccca gcctcaatga
     1981 ttgggcgaat tgtcttcgga tgaaccactt ttgccccaaa ataggctaat tccgcaattt
     2041 cccgatacga aagctgtgag atggtatgtg ccccaggaac agcacgagga tcagcggtca
     2101 ttacgccatc cacatccgtc caaatccaga catcgtcagc tggcaagtgt gctcccaaaa
     2161 ttgcggcagt gtagtcactc ccacctcgtc caagagtgat cgtaatccca tcgggcgttg
     2221 cggcgataaa tcccgtgata accgggattt ttccctgtcc caagatgggt tcaagaaccc
     2281 tgccgattgc ctgtcttgtc aattcaaaat ctggatgggc tgactgaaat tggttatctg
     2341 taatgatcag ctgagtagcc tctacaaatt gagctggcat ccccctgcta tccagagctg
     2401 cggccaatac gcgaacgctc aggcgctcac caagcgatgc caccgcatcc agcgcgcgag
     2461 gggtcgcttc tcccaacacc tggattgcct gacaaaggtt cgtgaactcg ccgataagat
     2521 ggttgatctc ttgttttacc tgcgcctgcc gagcaagatc gggcaagagg gctgctaata
     2581 tttcccagtg ctgcgtgcga attcgctggg caatttgatg gacgtactcg gtgctgccct
     2641 caaccgcttt cgaggcactt tccagcaata agttagtgac acctgccatg gctgaggtaa
     2701 ccaccaccat ccgcggccac tctgaccgcg cctgctcaac gatctcacat acctggcgga
     2761 atgcatcctc actacctaca gaagtcccac caaatttcat cactagcgta cgggaatcac
     2821 gcctctcttg catcactacc tcccgtataa gacatattcc tcagatcaat tt
//
LOCUS       DBNHOH010000634         1635 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1000184, whole genome shotgun sequence.
ACCESSION   DBNHOH010000634 DBNHOH010000000
VERSION     DBNHOH010000634.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1000184"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="AC16V7_11105"
     CDS             complement(<1..836)
                     /locus_tag="AC16V7_11105"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase FemX"
                     /protein_id="HIR6257710.1"
                     /translation="MKVTHITAERREEWNVFVAKEPSFALLQSWGWGEFKEKMGWKVF
                     RIGAEKEGRIVAGVQMLIRPIPPGLTSIAYIPRGPVGDWLNEEVLSLLLTELHQVARR
                     HRAVFLRIEPPLLNNSTYAAVLEKHHFHSSTYTNQPRATITLNLTPEIDEILATMRDK
                     TRYNIRYATKKGITVRVGDYDDLLTFYRLMRITSLRGGFSHQTLNYYRHEWETFARLG
                     QIKLFVASYQGKTVAANMSAVFGEHGAFLHGASSGEFANLQPNYLLMWEAMLWAKDQN
                     CHT"
     gene            complement(1355..>1635)
                     /locus_tag="AC16V7_11110"
     CDS             complement(1355..>1635)
                     /locus_tag="AC16V7_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HIR6257711.1"
                     /translation="LGWPLPPNLVQHYGTVKWIHLRRDFQSALYYWRRGELTLMDWWR
                     SVRGRKVDALFAWDDPGPFIGDLERAIRLFLSPEERRKRDYSTPLKQT"
BASE COUNT          395 a          398 c          392 g          450 t
ORIGIN      
        1 gtatgacagt tctgatcctt tgcccacagc attgcttccc acatgagcag ataattaggt
       61 tgtaagttgg caaattctcc agatgaagcc ccgtgtagaa atgctccatg ctctccaaat
      121 acagcagaca tattggctgc aacagtttta ccttgatatg aggcgacaaa taacttgatt
      181 tgtcctaatc tggcgaaagt ctcccactca tgccgataat agttcaacgt ttgatgactg
      241 aacccacctc ggagacttgt gattcgcatg aggcggtaga atgtcagcag gtcgtcgtag
      301 tcgcccaccc gaacagtgat cccctttttg gttgcatagc ggatgttgta gcgggttttg
      361 tctcgcatcg tcgccaggat ttcgtcaatt tccggtgtca aattcaatgt aatcgtggcg
      421 cgcggttggt ttgtgtaggt actgctgtgg aaatgatgtt tctctagcac tgcggcatat
      481 gtagagttgt tcagtaacgg cggttcgatg cgtagaaata cagcccggtg tctacgtgcg
      541 acttgatgta gttcggttaa taatagggat aatacctctt catttagcca atcgcctaca
      601 ggaccccggg ggatataggc tatactggtc aatccaggtg gtatgggcct gatgagcatc
      661 tgcacgccag ctacaattcg cccttctttc tctgctccaa ttcgaaaaac tttccacccc
      721 attttttcct tgaattcacc ccacccccaa gattgtaata gggcgaaaga tggctccttg
      781 gctacaaata cattccactc ctctcgccgt tcagcggtta tgtgcgtgac tttcattgga
      841 atacaacctg catcacaaaa tcgcgcctgc aataatctgg gtcgtgaaag cgaattttag
      901 ggtatctggc gaaaaaatcc ataagtaagc ttacttggtt cagcaatgat ggtttgtgaa
      961 tatttatatc acgcccgtag tttaccagcc tgccccaaat ttgggcttaa ctctcgcagg
     1021 ttgtacttat catcgggtat tccgctgccg caaaggaagc ctaatgccat ggcttgtggt
     1081 cttgagcatg ttcaaaccct tcgaacgttc cagggtatga agaataaccc cctgggtgtg
     1141 tttaccaaca agctctttcc aaatgtggct atctaagccg ctgggaagtt ccattgttta
     1201 gatgtgaatg gaaggccgac gtttaaccaa aaagcttggg ggaggccagc tcccccaaga
     1261 tctcctcccc cgcgttattt gggaaataag aagcagatcc tcgctatggg tgcggatttt
     1321 gagacgatat atccggaatc atttatgtgt gaagctacgt ttgtttcagc ggtgtgctgt
     1381 aatctctttt ccttcgttcc tccggggaga ggaataaacg gattgctctt tctagatcgc
     1441 cgataaaagg tcctggatca tcccaggcaa ataaagcgtc taccttccgg ccgcgcaccg
     1501 accgccacca atccatgagg gtaagctctc cccggcgcca gtagtataga gcagactgaa
     1561 agtcccgtcg gaggtgaatc cactttacag tcccataatg ttggacgagg tttggtggca
     1621 gtggccaacc caacg
//
LOCUS       DBNHOH010000635         1299 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_422392, whole genome shotgun sequence.
ACCESSION   DBNHOH010000635 DBNHOH010000000
VERSION     DBNHOH010000635.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_422392"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="AC16V7_11115"
     CDS             complement(<1..576)
                     /locus_tag="AC16V7_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HIR6257712.1"
                     /translation="MQSLTRTERDSLGEVEVPIQAFYGVQTQRAVLNFPISGLRPWRA
                     FIWAMATIKRAAAEVNRDLGLLDGSHADAIVQAAQEVIDDRWDEQFVVDPFQAGAGTS
                     HNMNTNEVIANRATQILGGDLGEYRVHPNDHVNMAQSTNDTIPTAIRLGALWRLNELL
                     EVVSELSEALSQKAIEFDEIVKSGRTHLQDAV"
     gene            689..>1299
                     /locus_tag="AC16V7_11120"
     CDS             689..>1299
                     /locus_tag="AC16V7_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6257713.1"
                     /translation="MLIHSASQLLTLAGGPQRGHDLGRLKIIPDGAVLIQDEDIVATG
                     PSDDLRAAYPNEPTLDASGRVVMPGFVDPHTHAIWAGDRAAEFELRLEGKTYLEILAA
                     GGGILSTVRATRTASLNKLLDQTRPRLWSMFNHGTTTAEVKTGYGLRSATELRMLQAL
                     LALEVEGPLELAFTFMGAHAIAPEYKENPDDYTDLICQTMLPML"
BASE COUNT          274 a          389 c          352 g          284 t
ORIGIN      
        1 gacagcatcc tgcaggtgcg tgcgaccaga tttgactatc tcgtcaaact cgatggcttt
       61 ttgactaagc gcctccgaga gctcactcac aacctcgagt agctcattca gccgccaaag
      121 cgcccccaga cgaatggcgg tggggatagt gtcattggtc gattgagcca tatttacgtg
      181 gtcattggga tggacacggt actcccccaa atctccaccc aagatttgcg tggcgcgatt
      241 ggcgatcacc tcgttggtgt tcatgttatg actggtgccc gcccctgcct ggaaggggtc
      301 gaccacgaac tgctcgtccc aacggtcgtc gatgacctcc tgggcagcct gcacaatagc
      361 atcagcatgg ctaccatcca gcaaccccag atcgcggttg acttcggctg cggcgcgctt
      421 gatagtcgcc atcgcccaga taaaagcccg ccagggtcgc aagccagata ttgggaagtt
      481 cagcacggcg cgctgggttt gcacgccgta aaacgcctgg atagggactt ccacctcgcc
      541 tagtgaatcg cgttcggtgc gggtgagtga ttgcatgagt ttctcctttt ccccagaaat
      601 taagacttat tttacccgaa ttttcagctg agccttcgat ctccagtgta cgatgcacgc
      661 aaagcatgct ttcgcatata atatccccat gctcatccac tccgcttctc aattactaac
      721 cctggccggc gggcctcagc gcgggcatga cctggggcgc ttgaaaatca tccccgatgg
      781 cgctgtactc atccaggatg aagatattgt ggcaactggt ccaagcgacg atcttcgagc
      841 cgcctaccca aacgagccaa ccctggatgc cagcggtcgc gtggtgatgc cgggcttcgt
      901 agaccctcac acgcatgcca tttgggccgg cgaccgcgcc gctgaattcg agctgcgcct
      961 ggaggggaaa acttaccttg agatcctggc agcaggcggc ggcattctct ctacggtcag
     1021 agccactcgc acagcctcgt tgaataaact gctcgaccag actcgcccgc gcctgtggag
     1081 catgttcaat cacggtacca ccacggctga ggtgaaaacc ggctacggtt tgcgcagtgc
     1141 aacggagctg cgcatgttgc aggctctgct cgccttggag gtggaagggc cgttggaact
     1201 ggccttcacc tttatgggcg cgcatgctat cgcaccagag tataaagaaa accccgatga
     1261 ttacaccgat ttgatctgcc agacgatgct gcccatgct
//
LOCUS       DBNHOH010000636         3552 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1192040, whole genome shotgun sequence.
ACCESSION   DBNHOH010000636 DBNHOH010000000
VERSION     DBNHOH010000636.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1192040"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2126
                     /locus_tag="AC16V7_11125"
     CDS             <1..2126
                     /locus_tag="AC16V7_11125"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HIR6257714.1"
                     /translation="QWIILSLGILITSGVELFQYMRGGDLVEVLVEWLIEVFFLTLLL
                     VVAIRLYAKVNQQLKRSQAVHPRQGSLIQLSTKLAASHDKEEICHILVKELQEMIDHA
                     KIEVFLIDHASGNRRRYGVGNEGHSEEPVDSARPTRLEIPLRAGQERLGGLLVEVEQA
                     RGVSEDEHAILVSAANLAVLAIVNARLFQEQRLQRLDAEERENERRERERSLTLFGEI
                     TQAALGSEDYPAMLQALADNLATAFEADGVLILLLEEGTGRLLGEAAFGPLRPTARTL
                     QYDPQEMILGKWVLDSGEAVAIQDAVVSPYIDQRVATEMQSRSILALPLVADKEKLGI
                     AIISYYSEHRFSPQEINAGEQAARQIALAIAKGRALAMAQYRAQELDALQKATAALLS
                     TLDLEQLLGQILDAAISAVPSAHRGSLHLIAHDTGQLQVRAIQGYTDSRIRAINRTGG
                     SHIARAVRERRSLMVEGPAAEVFVAPGEAGSGSREAVSSIIAPLLLGDETLGAISLES
                     YRPYAFIQPDLDLLVSFAATATTAIQNAQLHAEVQRQAITDTLTGLYNRRGFFELGRR
                     EVERAIRFGRPLTAVMFDIDYFKNVNDLYGHAVGDRLLINLTTRLLQELRQIDLLGRY
                     GGDEFVALLPETDLSNAMSVAERLRKVVASTKVTGPSASVNISLSAGVATLSNDCRDL
                     NSLLQQADKALYEAKRQGRDRVVSM"
     gene            complement(2128..2769)
                     /locus_tag="AC16V7_11130"
     CDS             complement(2128..2769)
                     /locus_tag="AC16V7_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257715.1"
                     /translation="MPASLEDHLKKDQIALFRSLDHPAKIQAFLDEIPYSAEDSNRSP
                     LKVLQDHQAHCLDGGLFAAAALRRLGFPPLVVDMYPDPGMDDDHVLAIFVVDKHYGAI
                     AKSNFVGLRYREPVYRGLRELVMSYFADFYNVDGVKTLRTYTRPLNLKSFDRQGWEWS
                     DDGVDAIEKRLLGLARHPLLKPEVAAGLSPMDPLSYKAGMLNVNYAGLYKPEK"
     gene            2954..>3552
                     /locus_tag="AC16V7_11135"
     CDS             2954..>3552
                     /locus_tag="AC16V7_11135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015998.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6257716.1"
                     /translation="MNATVEMTRAQPSITLSDVETTGLLTLYCHALESQSTNPILKDD
                     KAVEIARQLTPLLIDSKDKLLRKLATNKVDQRLVVHIALRAKQYDQYAKNFLDQNPDG
                     IIVNIGCGLDPRFFRIDNGSMIFFDIDLPEVIRFKKKILAESDRYRMIAASVFDYQWM
                     DEVAQYGSRPVMFLAEGVFMYLEAEKVKSLVLELQSRFPG"
BASE COUNT          813 a          886 c          992 g          861 t
ORIGIN      
        1 ttcagtggat tatcctcagt ttgggtatct taatcacctc tggagttgag cttttccagt
       61 acatgcgcgg gggagatttg gtggaggttc ttgtagagtg gctcattgag gtttttttct
      121 taactctgct gctggtagtt gctataagat tgtatgcaaa ggtgaaccaa caactaaagc
      181 gcagccaagc tgtgcatccg cgccagggct cattaatcca gctgagcacc aaactggcgg
      241 cttcgcatga taaagaagag atctgccaca ttttggttaa agaactgcag gagatgatcg
      301 atcacgcgaa aattgaggtt tttctgatcg atcatgccag cggcaaccgg agacgttatg
      361 gggttggaaa cgaggggcat agtgaagagc ctgtcgattc agcgcgccct acccggttgg
      421 agattccact gcgcgctggg caagagcggc taggtggatt gctcgttgag gtcgaacagg
      481 cgcgcggggt cagcgaagat gaacacgcaa ttctggtttc tgcggcaaat ctggctgttt
      541 tagccatagt aaatgctcgc ttgtttcagg aacagcgcct gcagcgcctg gatgccgaag
      601 aacgcgagaa cgagcggcgt gaacgtgagc gctccttgac gctgttcggc gagattaccc
      661 aagctgcttt gggctcagag gattacccgg ctatgctcca ggctctggcc gacaacctgg
      721 caactgcttt cgaagccgat ggtgtcttga tcttgctcct tgaagagggt actgggcgtt
      781 tgctcggcga ggcagctttt ggcccattgc gacctactgc ccggacgttg caatatgatc
      841 cgcaggagat gattttgggt aaatgggtgc tagactccgg ggaggcagtg gcaattcagg
      901 acgcggtcgt ctccccatat attgaccagc gcgtcgctac cgagatgcaa agccgatcta
      961 tcctggcttt acccctggtg gcggataaag aaaaattggg catagcgatc atatcttatt
     1021 attctgagca tcgtttctcc ccacaggaga tcaatgcagg tgaacaagct gcccgtcaga
     1081 tagccctggc aatagccaaa ggacgtgcct tggctatggc acaataccgc gcccaagagc
     1141 ttgacgccct gcaaaaggcc acggctgctt tgctctccac gctggatttg gagcagttgt
     1201 tgggacaaat actagacgcg gccatcagcg cggtgccctc ggctcatcga ggctcactcc
     1261 acctgatcgc ccacgataca ggtcaattgc aggtgcgtgc aattcagggt tataccgatt
     1321 cccgcatccg cgccatcaat cgtacaggtg gctctcacat tgccagggcg gtgcgtgaac
     1381 gccgttcatt gatggtggaa ggacctgcgg cggaggtctt tgtggcgccc ggtgaggctg
     1441 gctcaggatc gcgcgaggct gtctcatcga tcattgcccc cctgctgctt ggggacgaga
     1501 ccctgggagc aatatccctg gagtcctacc gcccctatgc cttcatccaa ccggacttag
     1561 atttgctggt gagcttcgct gctaccgcta ccaccgccat tcaaaatgct caattgcatg
     1621 ctgaggtgca gaggcaggcc attaccgata ccctaacggg cctgtataac cggcgtggct
     1681 tcttcgagtt aggtcgccgt gaagtcgagc gtgccatacg ctttgggagg ccactcaccg
     1741 ccgtcatgtt tgacatcgat tatttcaaaa atgtaaatga cctgtatggg cacgcggtgg
     1801 gtgataggtt gttaattaac cttacgacgc gcttattgca agagctacgt caaatcgatc
     1861 tgttggggcg ctacggggga gacgagtttg ttgctctgct gcccgagacc gacttgagca
     1921 atgccatgag tgtggctgag cgcctgcgta aagttgtggc ttctacaaag gtgaccggac
     1981 cgtccgcatc cgtgaatata tcacttagtg caggggtggc aaccctgagc aacgactgca
     2041 gggacctgaa cagtctgttg cagcaggcgg ataaggcttt gtatgaggcg aagcggcagg
     2101 gtcgagaccg ggtagtgagt atgtagacta cttttccggt ttgtacagcc cggcgtaatt
     2161 cacattcagc attccagctt tatatgagag agggtccatt ggcgaaaggc cggcggcgac
     2221 ttcgggtttc aacaaggggt gacgagccaa tccgagcaac cgcttttcga tggcgtctac
     2281 accgtcgtca ctccattccc atccctgtcg gtcgaatgat tttaagttca ggggacgggt
     2341 gtaagttcgc aaagtcttga ctccatctac attgtaaaaa tcagcaaaat atgacatgac
     2401 cagctctcgc aagccacgat agaccggctc gcggtaccgc agcccaacga aattcgattt
     2461 ggcgattgcg ccatagtgtt tatcaaccac aaatatagcc agcacgtgat cgtcgtccat
     2521 gccgggatct gggtacatat ctaccacgag aggtggaaaa cccaggcggc gcagcgctgc
     2581 tgcagcaaat aagcccccgt ccaggcagtg agcctggtgg tcctgaagca cctttagtgg
     2641 agatcggttg ctatcttcgg cgctgtaggg aatctcatcc aggaaagcct gaattttggc
     2701 tgggtggtct agagagcgaa atagggcgat ttgatctttc ttcaggtggt cttctaaact
     2761 ggctggcata tgtatggtta tctcctaggc ttggataaaa tttgcctggc tggtatttta
     2821 cacgttaatc tggatcggtg gtacggttgt cttgagattt ttttcggaga tcagtggcag
     2881 caagggttga caaactatcg aagtgtttgt ataatatctt tactgaccag gtggtcagta
     2941 aaggagaatg tatatgaatg ccaccgttga aatgacccga gcacagccgt cgattacgct
     3001 ctcggatgtg gagacaaccg gtttgctcac actctactgc cacgcccttg agagccaatc
     3061 gactaatcct atcctcaagg atgacaaggc ggttgaaatc gcccggcagc taactccgct
     3121 gttgatcgat tcaaaagaca agctactcag aaagctcgcc accaacaaag tggatcaaag
     3181 gctggtcgtg catattgccc tgcgcgccaa gcaatacgac caatacgcca aaaatttcct
     3241 tgatcaaaat cccgatggga tcattgtcaa tatcggttgc ggcttggacc cccgcttttt
     3301 ccgtatcgat aacggcagca tgatcttttt cgacatcgac ctccctgaag tgatccgctt
     3361 caagaagaag attctggcgg agagtgaccg ctatcgcatg atcgccgcct cggtttttga
     3421 ttaccaatgg atggatgagg tagcccaata tggcagtcgt cccgtaatgt tcctcgccga
     3481 aggagtgttc atgtatctcg aggcggaaaa agtcaagtcg ctggtattgg agcttcaatc
     3541 ccgtttccca gg
//
LOCUS       DBNHOH010000637         2775 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1384388, whole genome shotgun sequence.
ACCESSION   DBNHOH010000637 DBNHOH010000000
VERSION     DBNHOH010000637.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1384388"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="AC16V7_11140"
     CDS             complement(<1..185)
                     /locus_tag="AC16V7_11140"
                     /inference="COORDINATES: protein motif:HMM:NF016365.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 family protein"
                     /protein_id="HIR6257717.1"
                     /translation="MIGTIINIFAVLLGGSLGILVGARLPEKIRSTVVAGLGLFTAAI
                     GVQMFMQSGNVIIVLGSV"
     gene            complement(182..733)
                     /locus_tag="AC16V7_11145"
     CDS             complement(182..733)
                     /locus_tag="AC16V7_11145"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR6257718.1"
                     /translation="MSKFYTRTGDDGTTGLLGEGRVPKHHVRIEAIGSIDEASAAIGA
                     ARAICKGEGVNALLQSVQRDLYDLMAEIAATKENLPRFQSINMERVAWLEGEIDRISA
                     AVKLPKGFILPGDSPASGLLALARTIVRRAERRVSRLLHEGQVENVHLQRYLNRLSSL
                     CFALELVENQAAGLDSINLANPE"
     gene            985..1212
                     /locus_tag="AC16V7_11150"
     CDS             985..1212
                     /locus_tag="AC16V7_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257719.1"
                     /translation="MQIRCYHCHKPFALGKEAVYQALQEMKTSEMSHYNAYCPHCRRA
                     NRVSHQELLRAAPDWTPEEETTDQGESVSSS"
     gene            1238..2209
                     /locus_tag="AC16V7_11155"
     CDS             1238..2209
                     /locus_tag="AC16V7_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6257720.1"
                     /translation="MKNVRSFSSAEELKKALGGQQYIASDEIATIMYLSQELGKPLLT
                     EGPAGVGKTELAKAIAGATGRELIRLQCYEGLDETKALYEWEYAKQLLYTQLLRDKLQ
                     DTLAGASNLTEAADRLAGEEDVFFSMRFLLQRPLLKAILSEKPTVLLIDEIDRADAEF
                     EAFLLEVLSDFQVSVPELGTLVAVHRPLVVLTSNNTRELSEALKRRCLYLFINYPTIE
                     QELAVVRLKVSELSNKLARQVVEMVQNLRSMDLRKSPSISETIDWAKALVSLNAKSLD
                     QKTLETTLSVLLKHESDLQRVRRWMETPPSTGRPGDPDDPRMARWRN"
     gene            2288..>2775
                     /locus_tag="AC16V7_11160"
     CDS             2288..>2775
                     /locus_tag="AC16V7_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR6257721.1"
                     /translation="MEERIVKFITALRSAGVRVSLAESADAFTAVDLLGVRNRDTFRL
                     TLRATLVKDAKNIPIYEELFPLFFGSADAPPLMNLSDDLTPEEAEKIAQALRQFSEKI
                     RQMLERLMRGEQLSQEELERLGKFVGLNHIDDLRYREWMTQRLQKALRFAEVREALQE
                     LG"
BASE COUNT          678 a          684 c          749 g          664 t
ORIGIN      
        1 acgcttccta ggacgataat gacattccca gactgcataa acatctgcac cccgattgct
       61 gcggtaaaaa gtcccaagcc agccaccacg gtggatcgta ttttctctgg caatctggcg
      121 cccaccagga tgcccaggct gcctccgagg aggacagcga agatatttat tattgtccca
      181 atcattctgg atttgcgagg ttaatggagt ctagacctgc tgcctggttt tccaccagtt
      241 ccagggcgaa gcacaaagac gataagcggt tcaagtatcg ctgcaaatga acattttcca
      301 cctggccctc gtgcagcaaa cgggagacgc gccgctcggc gcggcggaca attgtgcgag
      361 ccaacgccag cagcccgctg gctggagaat caccgggcag gataaaccct ttgggtaatt
      421 taactgcagc gcttatgcgg tcgatttctc cctctaacca agccacgcgt tccatgttga
      481 tgctttggaa gcgaggcaag ttttctttgg ttgcagcaat ctctgccatc aaatcatata
      541 gatcgcgctg cacgctctgc aatagagcgt ttaccccctc gcccttgcat atggcgcgcg
      601 cggccccaat ggctgctgaa gcctcgtcaa tgctgcctat cgcctcaatg cgtacgtgat
      661 gtttggggac acgtccttcg ccgagcaagc ccgtcgtacc atcatctcca gtccgcgtgt
      721 agaattttga cataagggca attatacctg gcttttatag ggcaatcgca tgaggacgat
      781 ttcccatcac agaagagaga aaatggggtg ttttggtggg ggtataactc cgccaaaaca
      841 ccccaaacac tctatttgcg agattgcgca tgccactcag ggcatatgca attgccctgc
      901 tggcttttgt tttagtctat gtagaaccgg atagatactg tatactatag attgtgactg
      961 agagttagga taggagatca tgtcatgcag attcgatgtt accattgcca caaaccattt
     1021 gccttgggca aagaagctgt ctatcaggct ctacaagaga tgaagacaag cgaaatgagc
     1081 cactataacg cctattgccc gcattgccgc cgggcgaacc gtgtttctca tcaagaacta
     1141 ttgcgcgcgg ctcccgactg gactcctgag gaagaaacaa ccgatcaggg ggaaagcgtt
     1201 tctagctcat agccctttgg tcttaacagg tggatagatg aaaaatgttc gttcttttag
     1261 ctctgcggaa gagctaaaga aagctttggg tgggcagcaa tatattgcta gcgatgaaat
     1321 tgctacgatc atgtacctca gccaggagtt ggggaagcca cttctgacag aaggacccgc
     1381 gggtgttggt aaaactgaat tggcgaaagc gatcgcaggc gccacggggc gcgagctgat
     1441 tcgtttgcag tgctatgaag gcctggacga gaccaaggcg ctttatgaat gggagtatgc
     1501 caagcagttg ctctataccc agcttttacg tgataagctg caagatacgc ttgcaggcgc
     1561 cagcaacctg accgaagccg ccgaccgcct ggcgggagaa gaggatgttt tcttttcgat
     1621 gcgctttcta ttgcagcgcc ccttgctcaa agctatactc agcgaaaaac caaccgtctt
     1681 attaattgac gagatcgatc gtgctgacgc ggagttcgag gcctttttgc ttgaggtgct
     1741 gagcgatttt caagtcagcg ttccggagct gggtaccctg gtagctgttc accgcccatt
     1801 ggttgtgctg acgagcaaca acactcgtga gctcagtgaa gcgctgaagc gtcggtgcct
     1861 gtacctattt atcaattatc ccaccataga gcaggagctg gctgtagtac ggttaaaggt
     1921 gtcggagtta tctaataagc tggctcgcca ggtggttgag atggttcaaa acctgcgctc
     1981 aatggatttg cgcaaatccc ctagtatcag cgagaccatt gattgggcta aggccctggt
     2041 ttccttgaac gccaagagct tggatcagaa aacattggaa accacgttga gcgttctctt
     2101 aaaacatgag agtgatttac agcgtgtccg ccgctggatg gagacacctc cctccactgg
     2161 aagaccgggg gatcctgacg atccacgtat ggcgcgatgg cgtaattgag ggtgaagggt
     2221 ctcacgggat ctacaagaac tcctcttgct cgaccgggag ccccccaaaa attttatagg
     2281 tgctggtatg gaagagcgta ttgtcaaatt tattacagcc ttgcggtcag caggtgtacg
     2341 cgtaagcctg gctgagagtg cagatgcatt caccgcggtt gatttacttg gtgtgcgcaa
     2401 tcgcgatacg tttcgattga ccctgcgggc taccctggta aaagacgcca agaacatccc
     2461 aatctacgag gaactatttc cgcttttctt cggtagtgct gatgcgcctc cgttgatgaa
     2521 cctctcggat gatttaacgc ccgaagaggc ggagaagatt gctcaagctc tgcgccagtt
     2581 cagtgagaaa attcgccaga tgctcgagcg cttgatgcgt ggtgaacaac ttagccagga
     2641 agaattggaa cgcctgggaa aatttgttgg gctaaaccat attgatgacc tgcgctaccg
     2701 ggaatggatg acgcaaagat tacagaaggc cctgcgattt gccgaagtgc gtgaggcatt
     2761 gcaggaactg gggga
//
LOCUS       DBNHOH010000638         4013 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_679629, whole genome shotgun sequence.
ACCESSION   DBNHOH010000638 DBNHOH010000000
VERSION     DBNHOH010000638.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_679629"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /gene="tyrS"
                     /locus_tag="AC16V7_11165"
     CDS             complement(<1..1191)
                     /gene="tyrS"
                     /locus_tag="AC16V7_11165"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIR6257722.1"
                     /translation="MPEMTIDDQVQLLMQGTEYGDEELKQAMATELRQRLVEAKAEGR
                     SLRVYCGFDPRTSDLHLGHMVPMRKLRQFQELGHEVTFLVGNYTSLIGDPSDKDKLRP
                     ILTPEQVEENARTYAEQAYKILDKQKTLIRYNATWLSQLTFAELIKLASNFTLQQFLT
                     RENFRLRWDKGDPIYLHETFYSIMQGYDAYALRTDVQVGGTDQLFNIVTAARKVMSYL
                     DEKPNIAIILGILPGTDGEVKMSKSLGNHIPLLSTPEDMYGKVMSVPDKAMGLYFRLV
                     TRWTPPEIEEIESGMASGQLHPRDIKMKLAHEIVSIFQSEQAAERAQEAFVRVFQRGN
                     LPTDMPEFSLQDGQTVLDVLVEGNLVSSKSDGRRMIDQQGVRLDGEVLTDPNQPLTQP
                     GVLQV"
     gene            complement(1404..1913)
                     /locus_tag="AC16V7_11170"
     CDS             complement(1404..1913)
                     /locus_tag="AC16V7_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257723.1"
                     /translation="MITPDREYHPELIPRRGEWIAWGSTLVVSAAWIILRLSGQSVFV
                     AVPFLAITLLLVAMGISLGNWMDRRTVIHLGDDGVKFDNGLRNVNLSWPEVHQVRVLP
                     SRFGKKVQVFGEQVYFSFRTLGEVHLQGELKGRMGFEKGEEILRQIVLMSGLQIMERE
                     GEEIYYIRP"
     gene            2024..2527
                     /locus_tag="AC16V7_11175"
     CDS             2024..2527
                     /locus_tag="AC16V7_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR6257724.1"
                     /translation="MHAPVLVLNANFEPLNVCTTRRAIGLILSGKADLVLNGRGVIKT
                     VSTAYPRPSIIRLERMVKRPRPRIRLTKREILRRDDFTCQYCGQNSPHLTVDHIVPRR
                     LGGLHSWDNLVAACPPCNHRKGGRTLEQAQMHLLRIPSEPSSSAQYLFARHLNENIEW
                     VPFVNDW"
     gene            complement(2600..3355)
                     /locus_tag="AC16V7_11180"
     CDS             complement(2600..3355)
                     /locus_tag="AC16V7_11180"
                     /inference="COORDINATES: protein motif:HMM:NF049367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6257725.1"
                     /translation="MAKLIILGSSNAIPDEKHENTHMVLSGRERTVLIDCVSNPMVRL
                     PKAGLEFDNLTDIILTHFHPDHVSGVPLLLMCTWLQGRKRPLNIYGLGYTLDRVEKMM
                     EFYDWATWPHFFPISFHRLPEAEMTPVIDCDEFRIFASPVHHLIPNIGLRVEFLETGK
                     TMAYSCDTEPCREVVRLGSGADVLIHECSGATLGHTSAEQAGEIAQRAEAGALYLIHY
                     PTGKNDPGKLVPDAKKMFNGPVALAEDFMTLEF"
     gene            complement(3389..>4013)
                     /locus_tag="AC16V7_11185"
     CDS             complement(3389..>4013)
                     /locus_tag="AC16V7_11185"
                     /inference="COORDINATES: protein motif:HMM:NF014052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HIR6257726.1"
                     /translation="TWYRLEIAGTVRAMTVPIGIMSLALIAAIMIGVEQARVFVIPSE
                     LLNFPNIDQGFITGLEYIRLFSVSGIGTVWFHNLRAIFLATTMGVFSFGILAVVILML
                     PLMLVGYFMANLAQTGISPLLLFSTLVLPHGILEIPAIILAGASILRLGATLAAPSQG
                     ETIGEAWLRSLADWAKIMLGLVIPLLLGAAILEVLVTPRLAIWMFGG"
BASE COUNT         1037 a         1101 c          948 g          927 t
ORIGIN      
        1 tacctgtaaa accccgggct gtgtgagggg ctgattggga tccgtcaaaa cttcgccatc
       61 caagcgcacc ccctgttgat ctatcatgcg gcgcccatca cttttgctgc ttaccagatt
      121 gccttcaacc agcacgtcca ggaccgtctg accatcctgc aggctaaact caggcatatc
      181 cgttggaagg ttgccgcgct gaaaaacgcg cacgaaggct tcttgggcgc gctcggcggc
      241 ctgctcactt tgaaagatgg aaacaatttc gtgggcgagc ttcatcttta tatcgcgcgg
      301 gtgaagctga cctgatgcca tgcccgattc tatctcctca atttcgggcg gagtccagcg
      361 cgtcaccagg cggaagtaca ggcccatggc tttatctggc acgctcatca cttttccata
      421 catatcctca ggtgtggaga gcagggggat atggtttccc aatgacttgc tcattttgac
      481 ctcaccatcc gtgcctggaa gtatgcctag tatgatggca atattgggtt tctcatccag
      541 ataactcatc accttacgag ctgccgtgac gatattgaac aactggtctg tgccccccac
      601 ctgcacatct gtgcgcaagg cataggcatc gtacccttgc atgatagagt agaatgtttc
      661 atgcagataa atgggatcgc ccttatccca gcgcaggcga aaattctccc gcgtgagaaa
      721 ttgttgcagg gtgaagttcg aggccaattt aattaactcg gcaaaagtca gctgcgaaag
      781 ccaggtagcg ttgtagcgga tcaaagtctt ttgcttgtcc agtattttat acgcctgctc
      841 ggcgtaggtg cgggcgttct cctccacctg ctcgggtgtc aatatggggc gcaacttgtc
      901 tttatcggag ggatctccga tgagcgaggt gtaattgccg accagaaatg tgacttcgtg
      961 cccgagctct tgaaactggc gcaatttgcg catgggcacc atatgaccaa gatgcaaatc
     1021 actcgtcctt gggtcaaaac cacaatagac gcgcaacgac cgaccctcag ctttggcttc
     1081 gaccagccgc tggcgcagtt cggtagccat agcttgcttt aattcttcat cgccatattc
     1141 agtgccttgc atcagaagct ggacttgatc atcaattgtc atctctggca tatttcttaa
     1201 gccctccaaa ctatcttgga tatcttcact aggttcctaa gctacagtgc ctgttatcgc
     1261 taaataacag gagtttcgca attgcaaggg tggtcgggct tcacccgccg caacccgcct
     1321 tctgcctttt tcaagcagga aagtcatcaa caataaaccg cgccctctgc ggtgagggcg
     1381 cgggaactca ccacaaaagt cactcatggg cgaatataat aaatttcttc accctcacgc
     1441 tccatgattt gtagcccact cataaggacg atctggcgca atatctcttc acctttctca
     1501 aagcccatcc tgcctttcaa ttcaccctga agatgtacct ctcccaatgt gcggaaggaa
     1561 aagtatacct gttcgccaaa aacctggact ttttttccaa aacgggaagg gagcactcgc
     1621 acctggtgga cttcgggcca ggaaaggtta acattgcgca agccattgtc aaacttcact
     1681 ccatcgtcac ccagatgaat caccgttcgg cggtccatcc agttgcccag ggaaataccc
     1741 attgctacga gcaacagggt aattgccaaa aaaggcacgg caacaaaaac cgattgccca
     1801 gataagcgca gaataatcca ggcggcactc acaaccagcg ttgaacccca ggcgatccac
     1861 tccccccggc gcgggatcaa ttctgggtgg tactcacggt caggtgtaat cacgacaagt
     1921 aattataaca gcaggccttt cgagtgtata caaaaaatgc caggcgctta ttgacagcat
     1981 catagcatcc cgtgctataa ttccgagagt tacggaaagc cctatgcatg cacccgtact
     2041 cgtcctaaat gccaactttg agccactcaa cgtctgcaca acgcgtcgcg cgattggctt
     2101 gatattgagt ggtaaggcgg atctggtgtt gaacggtcgt ggggtaatca agacagtatc
     2161 aactgcctac ccgcgaccat caattatccg gctggagagg atggtcaaac ggcctcggcc
     2221 gcgcatccgc ttgacaaagc gcgaaatcct gcgcagggac gatttcactt gccagtattg
     2281 cgggcaaaac tctcctcacc taaccgtaga tcatattgtg cctcgacgtt tgggaggtct
     2341 gcactcatgg gataatctgg tagctgcctg cccaccctgc aatcatcgca aaggggggcg
     2401 cacgcttgag caagctcaaa tgcacttact acgcatacca agcgaacctt cgtcctccgc
     2461 acaatacttg tttgcacgcc acctgaatga gaatatcgag tgggttccct tcgtcaacga
     2521 ctggtaggaa aaaagcaccg aaacgaatca aaaatggggt tttttagaaa ccccattttt
     2581 tgtttaacgc cgatgtatat taaaattcca aggtcataaa gtcttcagcc agggccactg
     2641 gcccgttgaa catcttcttt gcgtcgggga caagctttcc ggggtcattt ttccccgtcg
     2701 ggtaatggat caaatacagc gctccggcct ccgccctctg ggcgatctcc ccagcttgct
     2761 ccgcagaggt gtgtcctaaa gtagcccctg aacactcgtg tatgagcaca tctgcaccag
     2821 aaccaagccg caccacctcc cgacaaggct ctgtatcgca tgaatacgcc atggttttac
     2881 ccgtctctaa aaattccact cgcaatccaa tatttggtat cagatggtgc accggagagg
     2941 caaaaatgcg gaactcatcg caatcaataa ctggtgtcat ctctgcctca ggcaggcggt
     3001 ggaaagagat cgggaagaaa tgcggccagg tcgcccagtc gtaaaactcc atcatcttct
     3061 ccacgcggtc cagcgtatag cccaagccgt agatattcaa tgggcgtttt cgcccttgaa
     3121 gccaggtgca catcagtaat agaggcacac ccgagacatg atccgggtga aaatgggtaa
     3181 gaatgatatc tgtcagatta tcaaattcca acccagcttt gggcagtcta accatcggat
     3241 tactgacaca atcaatcagc accgttcgtt cacgaccgga caggaccata tgggtatttt
     3301 cgtgtttctc gtctggtatg gcgtttgatg aacccaggat gatcaacttt gccacagcaa
     3361 ataccctccg caattcttca ggctgaggtc agcccccgaa catccaaatt gctagacgtg
     3421 gagtaactaa cacttcaagg atcgccgctc ccagaagaag gggtatcact agacccagca
     3481 tgattttagc ccaatctgcc agtgaacgca accatgcctc gccgatagtc tcgccctgag
     3541 aaggcgctgc cagggtagct cccaggcgca aaatggatgc tccagccagg ataatggcgg
     3601 ggatttcgag gatgccatga ggcagcacca gcgtggaaaa taataataat ggggatatac
     3661 cagtttgagc cagattggcc atgaagtatc ccacaagcat caacggcaac atcaaaatca
     3721 ccacagccag aataccgaag gaaaaaacac ccatcgttgt cgccaggaaa atagctctca
     3781 agttgtgaaa ccaaaccgta ccgatccctg acaccgagaa gagacggatg tattccaatc
     3841 ccgtaataaa gccctggtca atgtttggga aatttagcaa ctcactggga atgacgaaca
     3901 cccgcgcttg ttcaacaccg atcataatag cagcaattaa tgccaatgac attatgccga
     3961 ttggaacagt cattgcacgc acagtgccgg caatttccag tcggtaccag gtc
//
LOCUS       DBNHOH010000639         1169 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_230552, whole genome shotgun sequence.
ACCESSION   DBNHOH010000639 DBNHOH010000000
VERSION     DBNHOH010000639.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_230552"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            399..743
                     /gene="hypA"
                     /locus_tag="AC16V7_11190"
     CDS             399..743
                     /gene="hypA"
                     /locus_tag="AC16V7_11190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HIR6257727.1"
                     /translation="MHELPVTESILEISLRHATAAGAQRITDVYLVIGQLASIVDDSV
                     QFYWDIVSQNTPAEGARLHFRRIPTKLLCLDCNLDYIPASMDMSCPTCDGVNIKVITG
                     EEFYLESIEIED"
     gene            746..>1169
                     /gene="hypB"
                     /locus_tag="AC16V7_11195"
     CDS             746..>1169
                     /gene="hypB"
                     /locus_tag="AC16V7_11195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HIR6257728.1"
                     /translation="MPERLPVVEKILGANERLAAINRANLDAAHVYSLNFMASPGAGK
                     TSLIENTVKRLINQLNIAVIDGDLASSIDADRAAAAGATAVQINTGGECHLDAAMLQG
                     ALPQLNLNQIRLLIVENVGNLVCPASFPLGTHKSVLIAS"
BASE COUNT          338 a          278 c          245 g          308 t
ORIGIN      
        1 cccaatcaag aattttatgg cacagtaatc ataaaagtaa cctccccaga taatgtaaat
       61 ggctcacccc atgaggttaa tttaacattg gatatcatcg aaggcacaga taactctgtc
      121 ttcctgcctg tcgtcgtatc atccaaccca taattttcta gcaagtgcca ataggctgct
      181 ggaataggct tgtctccagc agcctatttt tattaatcgc cgcaagaata ttctcaaggc
      241 ttccgcgaaa agactctgat catctcagga aacttagaga ttgagattcg caaatatata
      301 aaacaagtta atttgagatt tatggtatga attacccgtg acaaatctca cttcagatta
      361 tcagcgcgct gtggtatcct gacaatggga taatatccat gcatgaacta ccggtaaccg
      421 aaagcatctt ggaaatttcc ctgcgacacg ccactgcagc cggcgcgcaa cggatcacag
      481 acgtttatct tgtcattggg caacttgctt caattgtcga tgattctgtc caattttact
      541 gggatattgt ttcgcaaaac actccagcag aaggagctag gctccacttt cgcagaattc
      601 caaccaaatt gttatgtcta gactgtaatt tagactatat acctgcctcg atggatatga
      661 gctgccccac ttgcgatgga gttaatatca aagtcatcac aggtgaagaa ttttatctag
      721 aatcgatcga aattgaggat tgagtatgcc tgagcgactg cctgtggtcg agaaaatcct
      781 tggtgccaac gaacgattgg ctgctataaa tcgcgctaac cttgacgcag cccatgtata
      841 ttcgttaaat ttcatggctt ctccaggtgc aggtaagaca tcactaattg aaaacacggt
      901 aaaacgccta atcaatcaac tcaatatcgc agtaatcgac ggagaccttg ccagcagcat
      961 tgacgctgac cgggcggctg cggctggggc aaccgccgta caaatcaaca caggtggtga
     1021 atgccacctg gatgcagcta tgctgcaagg tgcactccca caattaaact tgaaccaaat
     1081 ccggctcctc atcgtagaga acgttggtaa tttggtgtgc cctgccagct ttccgctcgg
     1141 aacacataaa agtgttttga tcgcctcaa
//
LOCUS       DBNHOH010000640         1348 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1192432, whole genome shotgun sequence.
ACCESSION   DBNHOH010000640 DBNHOH010000000
VERSION     DBNHOH010000640.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1192432"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1348
                     /locus_tag="AC16V7_11200"
     CDS             <1..>1348
                     /locus_tag="AC16V7_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR6257729.1"
                     /translation="IVFGLISGHVYLETAAVIITLIKLGKFLEARAKGRTSEAIKKLM
                     GLRAKTARIVRDGQEMEIATEEVRVGDIVIVRPGEKIPVDGVVVEGRSSIDESMLTGE
                     SLPVEKRPGDNVIGATLNKLGLLRFEATKVGRETALAQIIRMVEEAQGSKAPIQKLVD
                     RVSAVFVPAVIVIALFTFVIWYFFFPLPPASDVDAFTRALINMVAVLVIACPCAMGLA
                     TPTAVMVGTGKGAEMGVLLRSGEALERAGKITMVVLDKTGTITKGQPAVTDIIEGGGS
                     KGNKGQPSIPTHGFTSSSLEMLRLAASVEQGSEHPLGEAIWAEANNKALQLSPADAFS
                     AEVGNGVQAEVDGHTVLVGNLRMMEGRGIALNGLQTEVTRLQNEAKTAMLIAVDGEVR
                     GVIGVADTVKEGSPEAIQALHQMHLKVAMITGDNQKTAEAIARRVGVDRVLAEVLPG"
BASE COUNT          331 a          291 c          417 g          309 t
ORIGIN      
        1 catcgtgttc ggattgatca gcggacatgt ctatctggaa accgctgccg tgatcattac
       61 cctgattaag ttgggtaaat ttttagaggc gcgtgcaaaa ggtcggacaa gcgaggcgat
      121 caagaaatta atgggtctgc gcgccaaaac tgcccggatt gtgcgggatg gtcaggaaat
      181 ggagatcgcc acggaagagg tgcgtgttgg tgatattgtg atcgtccgcc caggtgaaaa
      241 aatccccgtg gatggcgttg tggtcgaagg acgctcaagt atcgacgaat ctatgttgac
      301 tggcgaatct ttgccggttg aaaagcgacc tggggataat gtgattggtg cgacgttaaa
      361 caagctggga ttgctcagat ttgaagccac caaagtagga cgggagacag ctctggcaca
      421 aattatccgt atggtagaag aggcccaggg cagtaaagcg ccgatccaga aattagtcga
      481 tcgggtttca gcagtgtttg tccccgcggt gattgtcatt gctctgttta cctttgtcat
      541 atggtacttc tttttcccac ttccaccggc ctcggatgtg gatgcgttta cccgcgccct
      601 gattaacatg gtggcagtgc tggtaattgc ctgcccgtgt gcaatgggct tagccacacc
      661 gacggcagtc atggtgggta ctggcaaagg tgcagaaatg ggtgtgctgt tgcgctctgg
      721 tgaggcgctg gaacgtgcag gtaaaatcac tatggtggta ttggacaaaa caggcacgat
      781 caccaaaggc cagccagcgg taaccgatat tatcgaaggt ggaggttcta agggaaacaa
      841 gggtcaaccc agcattccca ctcacggttt tacgtcttct tcgttagaga tgcttaggct
      901 ggctgcttct gtagaacagg gaagtgaaca ccccctaggg gaagctattt gggcagaagc
      961 aaataacaag gctctacaac ttagcccagc cgatgccttc agtgctgagg tggggaatgg
     1021 cgtgcaagct gaggtagatg ggcataccgt actggtcggt aacttgcgca tgatggaagg
     1081 gcgcggaatt gctttgaatg gtctacaaac cgaggtcact cggctacaga atgaagccaa
     1141 gactgccatg ctgattgcgg tggatggaga ggtgcgaggg gtgatcggtg tagcggacac
     1201 tgtgaaagaa ggctcaccgg aagcaatcca ggccttgcat caaatgcatc tgaaggttgc
     1261 catgatcact ggtgataacc aaaaaaccgc agaggcgatt gccagacgag tgggcgtgga
     1321 tcgcgtgttg gccgaggtgc tacctgga
//
LOCUS       DBNHOH010000641         1191 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1192446, whole genome shotgun sequence.
ACCESSION   DBNHOH010000641 DBNHOH010000000
VERSION     DBNHOH010000641.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1192446"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..969)
                     /locus_tag="AC16V7_11205"
     CDS             complement(172..969)
                     /locus_tag="AC16V7_11205"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009772880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR6257730.1"
                     /translation="MDIWIDQLSFTYPAGVTALQEVSLKISSGESVAIIGENGAGKTT
                     LVKHINGLLKPTSGQVLVGGWNTREYTVAQLASKVGYVFQNPDDQLFASTVWAEVLFG
                     PKNIGLTMEQATERADAALRAVGLIDSAQKHPYDLSISQRKKVAVASVLAMQTPMLVL
                     DEPTTGQDHNDTELVGQIVEDLKEQGRTVITITHDIDFCAEHFDRVVVMAQGMILLDG
                     PARDTLIQTEILAQTHVEPPQLMRLSEQLNMQERPLNVEEFIEFWCP"
     gene            complement(985..>1191)
                     /locus_tag="AC16V7_11210"
     CDS             complement(985..>1191)
                     /locus_tag="AC16V7_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257731.1"
                     /translation="TIIADGLPGEVLADEAVWSRGVGRTRYTQASHQARMNGLWPKGR
                     NLAVTLEQAVAGFQAAQTTYSKKS"
BASE COUNT          264 a          315 c          280 g          332 t
ORIGIN      
        1 acaatgcctg tttctccaat ctcctgccag ggccaaagcg tgctatactc catcgtagat
       61 cctcctcggt ttttggttgt ttgtgtgcac ccattttacc gagaagggtc tttttgtcta
      121 tctgtcaagc tttcttgaaa aagccattaa ttgaggggtc gatactgagt gttaagggca
      181 ccaaaattca ataaattcct ccacgttgag cgggcgttcc tgcatgttca attgttcgga
      241 taatcgcatc aattgaggcg gctcgacatg cgtctgtgcc aaaatttccg tttgtattag
      301 ggtgtcgcgt gctggaccat ctagcaatat catgccttga gccatgacaa ccactcgatc
      361 aaaatgttca gcgcaaaaat cgatgtcgtg agtgatcgtg attactgtcc ggccttgctc
      421 tttcaagtct tccacaattt gaccaacaag ctcggtgtcg ttgtggtctt gtccagtggt
      481 tggttcatcc aaaaccagca tgggggtctg catggctaac acagaggcca ccgctacctt
      541 ttttcgttga cttatggaga gatcataggg atgtttttga gccgagtcta ttaaccctac
      601 tgctcgcaga gcagcgtctg ctcgttcggt agcttgttcc atcgtcaagc caatgttctt
      661 gggtccaaaa agtacctccg cccatacggt tgatgcgaat aactgatcat ctgggttctg
      721 gaagacatag cccaccttag aagccaattg cgcgactgtg tactcccgcg tgttccaccc
      781 tcccacaagc acctggccag acgttggctt taataagcca ttgatgtgct tgaccagggt
      841 ggttttcccg gccccattct ccccaataat tgccacgctc tcccctgatg atatcttgag
      901 tgatacctct tgcagggcag tcacaccagc tggataagtg aatgatagtt gatcgatcca
      961 gatgtccatc gatagtgctg aatgctaact cttctttgaa tatgtagttt gtgcagcctg
     1021 aaaaccagcc accgcctgct ccagggtaac tgccaggttg cgccctttgg gccacaatcc
     1081 attcatgcga gcctggtggg atgcctgggt atagcgcgtt ctcccaaccc cgcgtgacca
     1141 tacagcctcg tcagccaata cctcgccagg tagaccatcg gcgataatgg t
//
LOCUS       DBNHOH010000642         3136 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1705555, whole genome shotgun sequence.
ACCESSION   DBNHOH010000642 DBNHOH010000000
VERSION     DBNHOH010000642.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1705555"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..2553
                     /locus_tag="AC16V7_11215"
     CDS             163..2553
                     /locus_tag="AC16V7_11215"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6257732.1"
                     /translation="MDTFLQELLTLLTQSPGNLTYHVVLSFSVAGSLQASLSQWRNNQ
                     FPQGRRLIIGLSLLLAIRLVIFVLAGIAWQGLVNEHILLPPVDRAANLISLLIIIWMW
                     CIPEPLRLADAATWLLGLLGLTGLALNLVWWLGQGSGIDYNGSWPDFIGEVVAISLIV
                     IGGVILLVRKPNSWGYGLGMLGIALAGHVAYLALPAPEGDYSGVLRMAQMAYYPFLFA
                     LPQRFPTSTQQVPIPAVPAEHKRPTSDPKLLESFLYLSSESEPDRICQAITRTLATAL
                     VADICLLLTPPNEQNEINIQCGFDLIREENLTGSSLSAKSVSLISTALTRRLPLRLPA
                     SSTSTDLRTLASHLHLERPGHMLFVPVVSADDSPIAGLLLLSPYSNRAWTSEDQTQLS
                     RFATAMAQLLQRTQMLAYLQIELNQTQEKLESAKAKAKALQSENEDLHYQVQIANEQG
                     EKNLLRAESLAAIIANQGETGLITPDQIAGVAPKGSTETEGSRLQSLLHAQTPDAHEP
                     TYMEGELRLALEEVARLKSALSEADQRHLAFINEQSDRLPINDKNDEIANIIQEMRQP
                     MSSVSGYTDFILSESIGILGALQRKFMERIKISTERMSQLVNELLRLTTVEMNLGKFT
                     ATGGADLYALVESAIAESMEAIRAKEINLRVDVAEKLPLLRADEHAIKQVLIKLLENA
                     KDVTPAKGEITLQASVQGGDHRQGYVLIQISDQGGGIPPEYVPLLFTRFSQANGKSIP
                     GTGGASSQLSIVKMLVENNGGRIWVDSQPNKGSTFSILLPVASENMFGDEPEDPLA"
     gene            2550..>3136
                     /locus_tag="AC16V7_11220"
     CDS             2550..>3136
                     /locus_tag="AC16V7_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR6257733.1"
                     /translation="MSSLRQVALGILTALFSITLVFGSVLIALTEGGVHLAMLPTPSQ
                     TAAPLVATPKPGEATFTPTLSPTPTETPAQPSSACKDTPAGWLSREVLPGETLDSIAA
                     EYGISVEILRQANCLTLDTLLPRSLISVPPPTQTSTPTATSTLRPTSVEQKKTSKQAT
                     RAPSRCSGHPARWVAYKVKRGDTIFRIAISYGLSS"
BASE COUNT          792 a          868 c          754 g          722 t
ORIGIN      
        1 gacagggttt tcaaaagttg aaagccttaa gcctaagttg gtaacaattt atccctcttt
       61 caatcgagat gtgatacaaa tctggtgatt ttgattggca taaatgttag tcttacctcc
      121 ttttctatgc ctatgcgaca tgctataatc gttgagatgg ctatggatac tttcctacaa
      181 gaactactca ctctactgac acaatctccc ggcaacctga cttaccatgt ggtgctgtct
      241 ttttccgtag ccggctcgct gcaggctagt ttgagccaat ggcgcaacaa ccagtttccc
      301 caggggaggc gtctgattat cgggctgagc ttgctgctgg cgattcgctt ggtgatattt
      361 gtcctggcgg gcatcgcctg gcaaggtctg gtcaacgagc acattttgct tcctcctgtg
      421 gaccgcgctg cgaaccttat cagtctactc atcatcatct ggatgtggtg cattcccgaa
      481 ccactccgct tggctgacgc cgctacctgg ctgcttggac tgctcggcct gaccggcctg
      541 gcgctcaact tggtctggtg gttggggcaa gggagtggta ttgactacaa cggctcctgg
      601 ccagacttca ttggagaagt ggttgccatc tccctgattg tcattggagg cgtgatattg
      661 ctcgtccgca aaccaaacag ctggggttat ggtcttggca tgctgggcat agctctcgct
      721 gggcatgttg cctacctggc actgcctgcg cccgaaggtg attattccgg cgtgctgcgc
      781 atggcccaga tggcatatta tcctttcttg ttcgctttgc ctcagcgatt cccaacaagt
      841 actcagcagg tcccaatacc cgccgtgcca gcggagcaca aacgccccac aagcgacccc
      901 aagctcttgg agagttttct gtacctaagt agcgaaagtg aacccgatag aatttgtcag
      961 gccatcaccc gaaccctcgc cacagccctt gtagctgata tttgtctgtt gcttacacca
     1021 cccaatgagc agaatgagat taatatccaa tgtggttttg atctgattcg cgaagaaaac
     1081 ctgacaggct catcactttc agcaaaatca gtatcgctga tatccaccgc cttgactcgc
     1141 cgcttacccc tgcgcctgcc ggccagcagc acttccacgg atttgcgaac tctggcaagc
     1201 cacttgcatc ttgaacgacc aggacacatg ctgtttgtgc cggttgtctc agccgacgac
     1261 agcccaattg cagggctttt attgctatca ccatactcca atcgggcttg gacgagtgaa
     1321 gaccagacac aactttctcg tttcgccacc gccatggcac agctcttgca acgtactcag
     1381 atgctcgcct atttacaaat cgagctaaac caaacccagg agaaactgga aagcgccaag
     1441 gcgaaggcga aagcacttca aagcgaaaac gaagatttac attatcaagt ccaaatcgct
     1501 aacgagcagg gtgagaagaa ccttcttcga gccgagtccc tggctgcaat catcgccaat
     1561 cagggtgaga cagggctaat cacccccgat caaatagccg gagtggcacc caagggaagc
     1621 acggagacag aaggcagccg actgcaaagc ctcttgcacg cccagacacc agatgcccat
     1681 gaaccgacct acatggaagg cgagctacgc ctggcgctgg aagaggtggc tcgattgaag
     1741 tcagctctct ccgaagcgga tcaaaggcat ctggccttta taaatgaaca aagcgacaga
     1801 ttaccaatca acgataaaaa cgatgaaatt gctaacatca tacaagaaat gcgacaacca
     1861 atgtcttcag tatccggtta tacagatttt atcttaagtg aatcgattgg tatcctaggc
     1921 gctctgcaac gtaaattcat ggagcgtatc aagatctcca ctgagcgcat gtcccaactg
     1981 gttaatgaac tgttgagatt gacaactgtt gaaatgaacc tgggtaaatt taccgccacc
     2041 ggaggggctg atctttacgc tttagttgaa agcgccatcg ctgagagcat ggaggcgatc
     2101 cgcgcaaaag aaatcaacct acgcgtcgat gttgctgaaa agttacctct actccgcgct
     2161 gacgagcatg ccatcaaaca agtcttgatt aagttgctcg aaaacgcaaa agacgtcacc
     2221 ccagccaagg gggaaatcac cctacaggct agtgtacaag gaggagatca ccgtcaaggc
     2281 tatgtgctta tccagatcag cgaccagggt gggggcatac cgccagaata tgtgccgtta
     2341 ttattcactc gttttagcca agccaatgga aagtctattc caggtacagg cggagcaagc
     2401 agccagctct cgatagtcaa gatgcttgta gagaataatg gtgggcgcat ttgggtagat
     2461 agccaaccta ataagggctc aacctttagc atcctattgc ctgttgcttc cgaaaatatg
     2521 ttcggagatg agcccgagga cccgcttgca tgagcagctt gcgtcaagtt gccctaggaa
     2581 tattgaccgc cctcttctcc attaccctcg tctttggtag tgtactcatc gccctaacag
     2641 aaggtggggt acacctggca atgctaccca caccatccca aactgcggcc ccgcttgttg
     2701 ccaccccaaa gcctggtgag gctaccttca caccaaccct gtcaccgacc cccaccgaaa
     2761 cccccgcaca gccttcctcg gcttgcaaag atacaccagc tggttggctt tcgcgggagg
     2821 tgttgcctgg ggaaacgctt gatagtatag cagcggagta tggcataagt gtagaaatcc
     2881 tgcgccaggc caactgccta accttagaca cgcttttacc taggagtctg atttcagtac
     2941 ctccaccaac acaaacctct actccaaccg ccaccagcac cctgcgtcct acatctgtcg
     3001 agcaaaagaa aacaagcaag caagccacgc gcgcaccgtc acgctgcagc ggtcatccag
     3061 caaggtgggt cgcctacaaa gtcaaaaggg gcgataccat attccgcatc gcaatttcct
     3121 acgggctgag tagcag
//
LOCUS       DBNHOH010000643         2375 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1000807, whole genome shotgun sequence.
ACCESSION   DBNHOH010000643 DBNHOH010000000
VERSION     DBNHOH010000643.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1000807"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..703)
                     /locus_tag="AC16V7_11225"
     CDS             complement(65..703)
                     /locus_tag="AC16V7_11225"
                     /inference="COORDINATES: protein motif:HMM:NF015944.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR6257734.1"
                     /translation="MNLGNRTTLIVLAIVVFVIFVCGASIASTYNGMVRSANNVRMVW
                     ADVESAYQRRLDLVNQTLPSVVAGAQQELAVFKTLRDQAAALSASFKRDELGQPIVPQ
                     GADAQNIQQQIVAFDRAWINALAYMADNPEIQSTQLYSDFMIQVEGSENRINVARRDY
                     NAAVNDYRNTTQTFPNNLAAGIFGFDPNQFAYFQAQPGAEQAPEVSFPTPVP"
     gene            complement(760..1608)
                     /locus_tag="AC16V7_11230"
     CDS             complement(760..1608)
                     /locus_tag="AC16V7_11230"
                     /inference="COORDINATES: protein motif:HMM:NF016424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HIR6257735.1"
                     /translation="MKYLNYFFSFFLFLVLTLPISTGVIAQGATPPVPSPLSEGSYVL
                     DQLGWLTPAHEETINTIVAKLDEENLAEIAVVTYDDCGPDSQSYRNTFFRTWGIGHKE
                     RNDGLLILVCWYGGDKSRRKLEQEVGYGMEGTIPDLLTSKVAEAHFIPVWKSGKDASR
                     AIADGDAGAALVAMVKAYDSVIRGDTPEELAKRPSSSSSSSDAGFQVIGWLLFAAILI
                     ILLVVWWKRIESGSDDGSSSDWGSSDDWSSSDRDSSGGWDSSDDSDRSSFGGGSSGGG
                     GSSSGF"
     gene            complement(1821..>2375)
                     /locus_tag="AC16V7_11235"
     CDS             complement(1821..>2375)
                     /locus_tag="AC16V7_11235"
                     /inference="COORDINATES: protein motif:HMM:NF012364.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HIR6257736.1"
                     /translation="RWVAFLPSRVDKRVPVPNRYFGVFQDGSLKIRGIESRRQDTPPW
                     IAQLQLSMLDCLSQAKSAGELPGCTPAIERVLRKAYSDLRLGRVPLEGLLVSQKLSRE
                     LDEYRVPSPAARAASQLVSVGKHLRPGQRVRFLYTLGEPGVRAWDLPDPPNPKTIDRL
                     YYRKLLMRAVETVLQPLGVDVVVG"
BASE COUNT          592 a          676 c          557 g          550 t
ORIGIN      
        1 ccagactttg tactattgct tcgaaaacgg gttgccaggc taatagtaca acaaggcact
       61 ttttctaggg cacaggtgtt gggaaactga cttctggagc ctgctcagcg ccgggttgtg
      121 cctggaaata ggcaaactgg tttggatcaa atccaaaaat cccagcagct agattgttcg
      181 ggaaagtctg cgtggtattt cgataatcat ttaccgccgc gttatagtca cgccgagcca
      241 cattaattcg gttttcagac ccctcgactt ggatcataaa gtccgagtag agctgggtcg
      301 actggatttc cgggttatca gccatgtagg ctaacgcatt aatccaggcg cgatcgaacg
      361 ctacaatctg ttgttggatg ttctgtgcat cagcgccctg tgggacaatc ggttgaccaa
      421 gctcatccct tttgaatgag gcagataatg ctgcagcttg atctcgaagg gttttgaaga
      481 cagccagctc ttgctgggct ccagcaacga cgctgggcaa tgtttggttc accaagtcaa
      541 ggcgacgttg ataagcagac tccacatccg cccacaccat ccggacattg ttcgcagacc
      601 gcaccatccc attgtatgta gatgcgattg atgctccaca tacaaatata acgaaaacga
      661 caatggccag cacgattaaa gttgttcgat ttcctagatt caccggcttg tcctccttct
      721 atgtttaaat ggcacggcgg agtatctttc actggtttgc taaaaacccg aactcgagcc
      781 gcctccgcca gatgaccctc caccgaaaga cgatctatct gaatcatcag agctatccca
      841 accacccgag ctatctcgat cactcgaact ccaatcatcc gagctacccc aatcactaga
      901 gctaccatcg tcagaaccgc tttctatacg cttccaccat accactaaca atataataag
      961 aatcgcagcg aataataacc acccgatcac ttgaaaccca gcatcggagc ttgatgagct
     1021 gctactaggg cgttttgcaa gctcttcagg cgtatccccg cggatcacac tgtcatatgc
     1081 cttcaccatc gccaccaggg cagctccggc atcgccatct gcaattgccc tgctggcatc
     1141 tttgccggat ttccagacag gtataaaatg agcctcggcc accttactgg tcagcaagtc
     1201 tgggatggtg ccctccattc cgtagccaac ctcttgttcc agcttgcgac gggatttgtc
     1261 accgccgtac cagcaaacca ggatcagcaa cccgtcgttg cgctctttgt gaccgatacc
     1321 ccaagtacga aagaatgtat tacggtagga ttggctgtcg ggaccacaat cgtcgtaggt
     1381 caccacagcg atctcagcca gattctcttc gtccaacttt gccacaattg tgttgatcgt
     1441 ttcctcatgt gctggagtca gccagccaag ctggtctaaa acataggaac cttcactgag
     1501 aggtgatggg actggtggag tggctccctg cgcgattaca ccagtcgaaa tcggcaaagt
     1561 cagcaccaag aataagaaga aactaaagaa gtaattgaga tacttcatct ttcaatcctc
     1621 cccaattggc aaattcaact cacaaccagc gatttgcgta gaatcaaaaa ccgatcgtta
     1681 ctttttatta actctcggct atacaatgcg gattgtcttg ttttagccac tgaaacacga
     1741 acagaccaag ctcataaaca attatatatt cattttactt cgagtcaatc gcatttttgc
     1801 aaccactatt ctggcaagga ttagcccact actacatcca ccccaagcgg ttgcagcacc
     1861 gtctctaccg cccgcatcag cagttttcgg taataaagcc ggtcgattgt cttcggattg
     1921 ggcgggtcag gcaggtccca ggcacgcacg cccggctctc ccagggtgta gagaaaacgc
     1981 acgcgctgcc caggcctcag gtgtttgccc accgatacca gctgagacgc cgcacgcgcc
     2041 gccggggagg ggacgcggta ctcgtccagt tcccggctga gcttctgcga aaccagcagg
     2101 ccctccagcg gcacgcgtcc caggcgcaga tcggaatagg ccttgcgcag cacgcgctca
     2161 attgccgggg tgcaacctgg cagttcaccg gctgattttg cctgcgacag gcagtccagc
     2221 atagacagtt gcaactgtgc tatccagggc ggcgtatctt gacggcgaga ttcgataccg
     2281 cgtatcttca acgagccatc ttgaaacacc ccaaagtagc ggttgggcac tggcacgcgc
     2341 ttgtccacgc gcgaaggcag aaaagccacc caacg
//
LOCUS       DBNHOH010000644         4808 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_936804, whole genome shotgun sequence.
ACCESSION   DBNHOH010000644 DBNHOH010000000
VERSION     DBNHOH010000644.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_936804"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..1317)
                     /locus_tag="AC16V7_11240"
     CDS             complement(199..1317)
                     /locus_tag="AC16V7_11240"
                     /inference="COORDINATES: protein motif:HMM:NF015715.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HIR6257737.1"
                     /translation="MSNPILPILTFMLNAFLKTWPYLLVTIPVAVAVNMSGASRYIRR
                     AFLAQPIAAILLATVVGAFSPFCSCSVIPLVAALLIGGVPLAPVMSFWIASPSMDPEI
                     FFLSVAMLGWELATVRLLATLALSIAAGFTTHWVIQRGWLGENILRTQKPPKVLKGRD
                     LLRAGWHFVQEKWTNLLRPNPAPLYLATEAACCNSQSVSTINPTGSQGARLLQIAVPA
                     TQGNSTVTQSTPALPQDSFWSRLWVETSKAVLLVVQFMALAFFLEALIELYVPQAWVA
                     SLVGRQSAYAILTAALLGVPVYTSNLTALPMISGLLGQGMSPAAALAFLIAGPTTTLP
                     AMSAVWGLVSRRVFALYVAFSLLGAILFGYVYQLLLSL"
     gene            complement(1387..1716)
                     /locus_tag="AC16V7_11245"
     CDS             complement(1387..1716)
                     /locus_tag="AC16V7_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR6257738.1"
                     /translation="MQELKQTIIPAACCTLEISGKEQDRLVKMFKALGNPIRFEIMKF
                     LVSHPGCITGDIVEFLPIAQATVSQHMKVLREAGWIKGTVEGPATSYCLSEENIAWFR
                     NQVETIF"
     gene            complement(1804..2460)
                     /locus_tag="AC16V7_11250"
     CDS             complement(1804..2460)
                     /locus_tag="AC16V7_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008097458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6257739.1"
                     /translation="MTNPIRLLIVDDQRLMREGLRTLLELEGGVEIVGEAAEGQAALD
                     AYTELKPDVVLMDIRMPGMDGVEATRRLCARWPEARVIILTTFDDDANVFEGLRAGAV
                     GYLLKDLSGQELANAVRTVYKGGALIEPTVAKRVMAEFARLHPPARNIDEGLPEPLSE
                     RELAILKLVSAGLSNREIGDKLGLTEGTVKNYVTNILQKIGVRDRTQAALRGRELGLI
                     "
     gene            complement(2457..3674)
                     /locus_tag="AC16V7_11255"
     CDS             complement(2457..3674)
                     /locus_tag="AC16V7_11255"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6257740.1"
                     /translation="MNSFSFTAHSQYNYDLMNNEMIQVRDPRLLNLTVYLIVMIMVII
                     SFISLPNGMAKGTAITLCVAFGLVHAFGFRYADTPRRLAIYFLAQVVITLALLILSSP
                     SDVFNLFLYVLGLEAVMILPTRPAAAWMVGFYLLDSLSAIWSRGSSGVTAVLFYGAAF
                     ILTALLGYALRQAEIARRKNQDLLTELKTTQRQLQDLAVTEERTRLAREMHDSLGHRL
                     TVSIVQLEGAQRLIPTDPRRAARMIGTMRDEMKEALAELRRTVAALRAPITEDIPLHI
                     ALSSLAQSFQQNTGIPTQFSISPDFPAMPEAYRLAFYRVAQEGLTNIQRHASAQNAWL
                     QLSADGQVTSLVIEDDGKGIMSQDENSAGTGLLGLRERAAQLGGEFRLEERPGGGTQL
                     TFSVPLPKLGMNL"
     gene            complement(3786..4586)
                     /locus_tag="AC16V7_11260"
     CDS             complement(3786..4586)
                     /locus_tag="AC16V7_11260"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR6257741.1"
                     /translation="MNSIRSFIKKNPIVTFAFLAYLLSWWSVPFADGRLIPYGPALAA
                     VIVLASTAGRKDLGNLWRRVTHWRVAWYWYLIGPGIIAGCQSVAFVLNVLLGATVKSY
                     PALPSMGTIITLLLLGGLWEEPGWTGYLLPRLQERFANRSNGALLAALVTGVFRAIWH
                     LPLYIYGHIPWFDVVVFEIALQLIIAWLFNRSGGSVLIVMLFHLASNIFGAVFNSSMF
                     TGTEWTNFSALFMALAALVALGILWRSGPNLGQTGTQNQFAVIDQSAG"
     gene            complement(4615..>4808)
                     /locus_tag="AC16V7_11265"
     CDS             complement(4615..>4808)
                     /locus_tag="AC16V7_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257742.1"
                     /translation="IKGSHILTPEETLMKTNLPTSKEKRPKRKLLIWMQRILSGLVIT
                     LVVLSAVKTVRTGQLLASR"
BASE COUNT         1159 a         1302 c         1308 g         1039 t
ORIGIN      
        1 gaccgtctcc aggccatcat ccgggcgcgc gacgccgggc tgggctaacg caattctaag
       61 aattaattct atgttgagag gggtgtcgtt gtttgttccc ctgccgaatt gaaatccgcc
      121 aatccaccaa tactccgaaa ttcgcggaaa ttggcggata taagaagctt tttgctgaac
      181 gtggcgtata tccccgatct ataagctcag caatagctga tacacataac cgaacaggat
      241 cgcgcccagg agcgagaagg ctacatacag ggcgaagacc ctcctggaga cgagccccca
      301 caccgccgac atggctggca gggtggttgt agggccagcg atcaggaatg ctagcgcggc
      361 cgcggggctc atcccctgcc ccagcaagcc gctgatcatg ggcagggcgg tcaggttgct
      421 ggtgtagacg ggcacgccaa gcagggcagc tgtcaggatg gcataggcgc tctggcgccc
      481 gaccaggctg gcaacccaag cctgtgggac atataactcg atgagcgctt ccaggaaaaa
      541 ggctaaggcc ataaactgga cgaccaggag aaccgctttg ctcgtctcaa cccacagccg
      601 agaccagaat gaatcctggg ggagagccgg tgtgctttgg gtgactgtag aattcccctg
      661 agttgcgggc acggcgatct gcagcaatct tgcaccttgt gagcccgttg gattgattgt
      721 agagacgctc tggctgttac agcaggcagc ctctgtggcc aaatacaggg gtgctggatt
      781 gggccgcaat agattagtcc atttttcttg cacaaaatgc cagccggccc gcagcagatc
      841 gcggcctttc agcaccttgg gcggcttttg tgtgcgcagg atattttccc ctagccagcc
      901 tctttgaatg acccaatgcg tggtaaatcc ggcggctatg ctgagagcca gagtggcgag
      961 caggcgcact gtagccagct cccacccaag cattgccacg ctcaggaaga agatctccgg
     1021 atccatggaa ggagacgcaa tccagaacga catcaccggc gccaggggca cacctccaat
     1081 caagagcgcc gccacgagcg gtatcacgct gcatgagcag aaggggctga aggctcccac
     1141 cacggttgcc agcaagatgg cggcgatggg ctgggccaga aaagcgcggc ggatatacct
     1201 ggacgccccc gacatgttta ccgccacggc aactggtatg gtcactaaaa gataaggcca
     1261 ggtcttaagg aaggcgttga gcatgaaggt caggatggga aggatagggt ttgacataca
     1321 acctcctata tcgtatattt acgatttatt cgagcaaaaa aatagacagt aagaaaagat
     1381 ccagctttaa aagatggttt cgacctggtt gcggaaccag gcaatatttt cttcgctcaa
     1441 gcaatagctg gtagccggcc cttcgaccgt acccttgatc cagcctgcct ctcgaagcac
     1501 cttcatatgc tgagagacgg ttgcctgggc aataggtaaa aactcgacga tatcgcccgt
     1561 gatgcaaccc ggatgggaga ccaaaaactt catgatctcg aagcgaatcg ggttccccaa
     1621 tgccttaaac atcttgacta atcggtcttg ttctttccca gaaatttcaa gtgtgcagca
     1681 agctgcgggg atgatggttt gttttagctc ttgcatagaa cctacttata tcgtaaatct
     1741 acgatagatt atatcaaaca ttctactctt gtcaagggta ttggcgataa atagcacatt
     1801 gggctaaatc aaacccaatt cacgaccgcg caaggcagct tgagtgcggt cgcgcacgcc
     1861 gattttttgt agaatattgg tgacgtagtt cttgactgtg ccttcggtta atccgagctt
     1921 atcgccgatc tcgcgattgc tgagcccagc ggagacaagc ttgaggatag ccagctccct
     1981 ttcggatagg ggctcgggaa gaccttcatc gatgttgcgg gcaggagggt gaaggcgggc
     2041 gaattctgcc attaccctct tggcgacggt cggctcaatt agcgccccgc ctttatatac
     2101 cgtccgaact gcatttgcca gctcctgacc ggagaggtct ttgagcaagt aacccactgc
     2161 tccggcgcgc aacccttcga acacgttggc gtcatcatcg aatgtggtca ggataatcac
     2221 ccgagcctca ggccaccggg cacaaagacg ccgggttgcc tccacaccgt ccatgcccgg
     2281 catgcggata tccatgagca ccacatctgg ctttaactcg gtatacgcat ccagggcagc
     2341 ctgcccttcc gccgcttcac ccacgatctc aactcccccc tcgagttcca gtagggtccg
     2401 caagccttcg cgcatcaggc gctggtcatc aactatgagc aatcgaattg ggttggtcat
     2461 aagttcattc ccagtttcgg caaaggtacg ctaaaggtca attgcgtgcc gccaccgggt
     2521 ctctcttcaa gtcgaaactc tccacccagc tgagccgctc gctccctcaa cccaaggaga
     2581 ccggtgccag cgctattttc gtcctgactc attattcctt taccgtcatc ttcgatgacc
     2641 aaagaggtca cctgcccatc ggcacttaat tgcagccacg cgttttgcgc gctagcatgg
     2701 cgctggatgt tggtcaaccc ttcctgggct acccgataga aagccagccg atatgcttcg
     2761 ggcatggcag ggaaatctgg ggaaatagag aattgcgtcg ggatgccggt attctgttga
     2821 aagctttggg ctagagatga caaggcgata tgcaatggaa tatcttcagt gatgggagcc
     2881 cgcagtgcgg caacggtacg gcgcagctca gccagggctt ctttcatttc atcccgcatt
     2941 gtcccgatca tgcgcgccgc gcgccttgga tccgttggga tgaggcgttg cgcgccctcg
     3001 agttgaacga tgctgacggt gagccggtgt ccaagcgagt cgtgcatctc ccgcgccagc
     3061 cgtgtgcgct cttcggtcac agctaagtct tgcagctggc gttgggttgt tttcagctct
     3121 gtgagcaaat cttggttctt gcggcgggcg atctcagcct ggcgcaaggc gtatcctaat
     3181 agagctgtca gtatgaaggc ggcaccatag aaaagcacgg ctgtaacccc acttgacccc
     3241 cgactccaaa ttgcgctcag gctatcgagc aaataaaagc cgaccatcca ggctgccgcg
     3301 ggtcgggtag gcaagatcat cacggcctct aagcccaaga cgtacaagaa caagttgaaa
     3361 acatcgctcg gggaagacaa tatcagtaag gccagtgtga tgacaacctg tgcgaggaag
     3421 taaatcgcca accgccgtgg ggtatcagcg taccgaaatc caaaggcatg taccaagccg
     3481 aaggcgacac atagcgtgat ggcggttccc tttgccattc catttggcaa gctgatgaag
     3541 ctgatgatca ccatgatcat cacaatcagg taaaccgtca gattcagcag tcgcgggtcg
     3601 cgaacctgga tcatttcgtt gttcatgaga tcataattgt actgcgaatg agcagtaaac
     3661 gaaaagctgt tcatgtcttc tcccccgtta tcgacgcaga gcagcaaggt cgaaaattag
     3721 gtgtttgtag ggggtcgcta cacacgaccc cccgcaaact gcacacgaac aagcttgcca
     3781 tccagttatc ccgcactctg atctataacg gcaaattgat tttgcgtccc ggtctgcccc
     3841 agattgggtc cagatctcca tagaattccc agcgcgacca gagctgccag ggccatgaac
     3901 agcgcggaaa aattggtcca ttcggtgcct gtgaacatgg aagaattgaa tacggcacca
     3961 aaaatgttgg aggcgagatg gaataacatc acgatcaaga cgctgccgcc gctccggttg
     4021 aatagccagg cgattatcag ctgtaaagca atctcgaata cgacgacatc aaaccagggt
     4081 atgtgcccgt agatgtagag cggtaaatgc cagatggctc gaaatacacc cgtcacaagc
     4141 gcggctagca gagcgccatt cgagcggttg gcaaagcgtt cctgcaatct gggcagcaaa
     4201 taaccggtcc agcctggctc ctcccacaaa cctccgagca gcaggagtgt gatgattgtt
     4261 cccatcgatg ggagtgcagg atagctcttt acggttgcgc ctaacagcac attgagcaca
     4321 aaagccacgc tttgacagcc ggcgatgatc cctggcccaa tgaggtacca ataccaggcg
     4381 acgcgccagt gtgtcacccg gcgccataaa ttcccaagat ccttcctgcc tgccgtgctt
     4441 gccaatacga tcactgccgc cagagctggc ccataaggga tgaggcgacc atctgcgaaa
     4501 ggaaccgacc accaactgag caggtaggcg aggaaagcaa acgtaacgat cgggtttttc
     4561 ttgatgaaag agcggatgct gttcatggtt gtctcctgtg aaaatttgat tagcttatct
     4621 cgatgccagt agctggcccg tgcggactgt tttgacagcg ctgagtacga ccagcgtaat
     4681 gaccaggcct gacaagatgc gctgcatcca gataagcagc ttgcgcttgg gtcgtttttc
     4741 tttggaagtg ggtaaatttg ttttcataag ggtctcctct ggcgtgagaa tatgtgaacc
     4801 tttgatgt
//
LOCUS       DBNHOH010000645         2447 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_550654, whole genome shotgun sequence.
ACCESSION   DBNHOH010000645 DBNHOH010000000
VERSION     DBNHOH010000645.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_550654"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="AC16V7_11270"
     CDS             complement(<1..979)
                     /locus_tag="AC16V7_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257743.1"
                     /translation="MSKSSTKVLVATFLIMALMISACEDTSFVGDPLLSQVHLIDPTF
                     REFYDLFGAEETLGPGISPLFAHRNIYYQYTTAALLMHDPGAPASQRFSLAPLGMDMD
                     IDDPPVSPPDDPKQRYVEGHIIFNLFVPLYEELGGARYVGVPISEVHYNPEKRRYEQF
                     FANLGFYWLEGDSPDQVHLLAYGAWKCDAYCRSPHPESSVVILPHHTAKRFNGAVDRL
                     GADFTGRAITDEYATPDGFNEQVFENVVMVVDPEQPSRVMLRSITERLGYMPDPMVAP
                     SQPKDYSFYEVQANRGYNVPKRFEAYIIQHGGYEVSGAPIGEIAHLKDAV"
     gene            1303..>2447
                     /locus_tag="AC16V7_11275"
     CDS             1303..>2447
                     /locus_tag="AC16V7_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257744.1"
                     /translation="MPKDTNPHPFESDRSRLGIHYFPDTVHYRESDLRTWLPELKKMG
                     AAWLTLCAPPDRAIPEIFLRGLMRDGIEPILHFHLPLSHQFQDGTLGLLFRAYASWGV
                     RYTVLFDRPNQRSAWPASTWAQSDLVERFLDIFLPVANAALQAGLTPVFPPLEPGGDY
                     WDTAFLRAALQGMERRDQAELLEAIHLSAYAWVDEHPLEWGEGGPERWPGAHPYFTPP
                     DQQDQRGFHIFDWYLAEAQAALGKPMPIFLLAAGRRLHNTDESPATEIDEIEHANINL
                     SIAKQFMAGASSDSISEPISPFVLACNFWLLTADPSEPSANQAWFQPEDYHLPIVAAL
                     HKLVRPGDAQQPDQSTPAHQLESKPDLHPPSSTLEDTHPSPVEPKII"
BASE COUNT          614 a          674 c          594 g          565 t
ORIGIN      
        1 aaaccgcatc cttaaggtga gcaatctcac caattggcgc cccgctcacc tcataaccac
       61 catgctggat gatatatgct tcaaaacgct tggggacgtt gtaccctcga ttagcctgca
      121 cctcatagaa agaataatcc ttgggctggc tgggggctac catcgggtca ggcatgtaac
      181 cgaggcgctc tgtgatcgac cgcaacatca ctctgctagg ttgttcaggg tcgactacca
      241 tcacgacatt ttcgaagacc tgctcattaa aaccgtcggg tgttgcatat tcatcggtga
      301 tggcgcgccc ggtgaaatca gcacccaagc gatccactgc cccattaaaa cgttttgccg
      361 tgtggtgcgg caggatcact acacttgact ccggatgagg agaacggcag tacgcgtcgc
      421 acttccaagc gccgtatgcc agcagatgca cctggtcggg cgaatcgcct tctaaccagt
      481 aaaatcctag gttggcgaag aactgttcgt atcgccgttt ttcggggttg taatgcactt
      541 cgctgatcgg aacacccaca tagcgggcgc cgcccagctc ctcatacagg ggcacaaaca
      601 ggttgaaaat gatgtggcct tcgacatagc gctgcttggg atcatctggc ggggatactg
      661 gtggatcatc gatatccatg tccatcccaa gaggtgccag tgaaaaacgc tggctggcag
      721 gagcgccagg atcatgcatc agtaatgcag cagtagtata ttgataatag atatttctgt
      781 gtgcaaagag aggggaaatt cccggaccga gtgtttcctc tgccccaaat agatcataaa
      841 attcccggaa agtcggatcg attaaatgca cctgggacaa gaggggatcg ccgacgaagc
      901 tggtatcctc gcaagccgag atcattaaag ccattatcaa aaaagttgca acaagcactt
      961 tggttgacga tttggacatt taatatttct cgagacaacc gaataaacta ttgctcttta
     1021 acactggatc atccaaccac atgcgtataa aaagcgcgag caacggcagc tatcttataa
     1081 gaagtttcaa gagaaattgc aggtgtatca tgatgtcctt aaggtaacct taacgaccac
     1141 tccctagtat atactgctgt ccttaaatta taactgacac ttttgtaagg aatcgacgtt
     1201 attaacagga ttttgtcagg aatcccctaa aaaaagccct ttatggcaca cctcttgcat
     1261 cgatataggt gccaattccc cttttaaggg gaggagcgtg ccatgcctaa agacacaaac
     1321 ccccatccat tcgagagtga ccgttcacgc ctcggcattc attattttcc agacactgtg
     1381 cattaccggg agagtgatct ccgcacctgg ttgccagaac ttaaaaaaat gggagctgcc
     1441 tggttgaccc tgtgtgcacc accagatcgc gcgataccgg agattttctt gcgcggtcta
     1501 atgcgcgatg ggatcgaacc tatcttgcac ttccacttgc ctttatcaca ccagttccaa
     1561 gatggcaccc tgggattgtt attcagggca tacgccagct ggggcgtacg ctataccgtc
     1621 ttgttcgatc gccccaatca gcgcagcgcc tggccagcct ccacatgggc acagtccgat
     1681 ctggtggaac gcttcctgga catcttcttg ccggtcgcca atgccgcctt acaagccgga
     1741 ttgaccccgg tcttccctcc gctcgaaccc ggtggggatt attgggatac agccttcctt
     1801 cgagccgcct tgcaggggat ggagcggcgc gaccaggcag aattgctgga agccatccac
     1861 ttaagcgctt atgcctgggt cgatgagcac ccattagagt ggggggaggg cggaccggag
     1921 cgttggcctg gtgcgcatcc atatttcact cccccagatc aacaagatca acgcggcttt
     1981 catattttcg attggtatct ggctgaagca caagccgctc ttggcaagcc gatgccaatt
     2041 ttcctgctgg ctgcgggtcg ccgcctgcac aacaccgatg aatccccagc gacagagatc
     2101 gacgaaatcg aacatgccaa cataaacttg agcattgcca aacagtttat ggcgggcgca
     2161 tcatctgaca gcataagcga accgatctcc ccatttgtcc tcgcatgcaa tttttggttg
     2221 ctcactgccg acccttctga gccttcagct aaccaggctt ggtttcaacc agaagattat
     2281 caccttccca tcgtggcagc gttgcacaaa ctggtaagac ctggagatgc tcagcaacca
     2341 gaccagtcca caccagctca ccaactggag agcaagcctg atctccaccc gcccagcagc
     2401 acgctggaag acacgcatcc atcacctgtt gagcccaaaa ttattag
//
LOCUS       DBNHOH010000646         1968 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1192832, whole genome shotgun sequence.
ACCESSION   DBNHOH010000646 DBNHOH010000000
VERSION     DBNHOH010000646.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1192832"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1443)
                     /locus_tag="AC16V7_11280"
     CDS             complement(49..1443)
                     /locus_tag="AC16V7_11280"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6257745.1"
                     /translation="MKGFQIVIRMRTFLIIWFGQLISIIGSGLTGFGLSVWIFEKTGQ
                     ALPIALNALFFNLPRVLLAPIAGAIADRYNRRHIMILADSGAAVTTLGVVALLFTGRL
                     EVWHIYLSTAISSCFGAFQDPAYRASITMLVPKEDLARAGGLDQMSFAVQSILTPLLA
                     GVLYAGFGLKAIILIDFVTFFFAVGALALVHIPQPKATSVAAEDRQRNRMWREAAFGW
                     KYLLERPGLFGLLWYYAVVNFFLTLSGVLFAPLVLSYGTPTEMGVVQTFGGVAMLVGG
                     LIMSIWGGPKSRRIWGVIAAIALSGFGYLLAGLRPSTLLIASAQFVILFFIPISAAIS
                     QAVWQMKVAPEIQGRVFAIRGMIAFSIMPVANIAAGILADKVFEPLMADGGILSGTII
                     GATIGVGAGRGIALIFIISAFFLWLSSLYAFATPRIRNLEVEIPDAIPDKPREAQPEV
                     GGVQEREMAPALGG"
     gene            complement(1551..>1968)
                     /locus_tag="AC16V7_11285"
     CDS             complement(1551..>1968)
                     /locus_tag="AC16V7_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257746.1"
                     /translation="DTTVKANVIEKHYWITAYEFKLAEALCNFSTPEGQNSVTTTMVA
                     PIETTLGDIKRSLKARAYALDHGAKQHPREVVIYRELRSIPDELATKFIARLNEVMDE
                     FNAYENQERSESVQTHALTIAFYPSFYFENPNKQDD"
BASE COUNT          492 a          524 c          498 g          454 t
ORIGIN      
        1 acatatgcta cattttttga ctgctataca aattaatgcc gcgcctgatt accctcccag
       61 agcaggcgcc atttctctct cttgaacacc accaacttct ggttgagctt cgcggggctt
      121 gtccggaatg gcgtcgggga tctccacttc caggtttcgt atgcgcggtg tagcaaatgc
      181 gtataggctg gataaccaca agaagaatgc ggagataata aatatcagag cgatgcctcg
      241 ccctgccccc acgccgatgg ttgcgccgat aattgtgcca ctcaggatac cgccatccgc
      301 catcaacggc tcgaaaacct tatccgccaa aattccggca gcgatattgg caaccggcat
      361 gatggaaaaa gcgatcatgc cgcggatagc aaaaacgcga ccctggattt cgggtgctac
      421 tttcatttgc caaaccgcct ggctgatcgc cgcagatatg ggtatgaaga acaggatcac
      481 aaattgagcg gaagcgatca acaatgtaga cggccttaat cctgcgagga gatagccaaa
      541 accgctcagc gcgatggctg cgataacacc ccaaatacgg cggctctttg gccctcccca
      601 aatactcatg atcaagcccc cgaccaacat agccactccc ccgaacgtct ggaccacacc
      661 catctcggtc ggggtgccgt atgagagaac aagcggcgcg aaaagtacgc cagaaagggt
      721 caaaaagaaa ttgacaactg cgtagtacca cagaagaccg aaaagcccag ggcgctcgag
      781 cagatatttc cacccgaagg ctgcctcgcg ccacatcctg ttcctttgcc tgtcttccgc
      841 tgctacgctt gttgccttgg gttgcgggat gtgcaccagt gccagggcgc cgactgcgaa
      901 gaagaacgtc acaaaatcga tcaggatgat cgccttcaag ccaaacccgg cgtatagcac
      961 gcccgcaagc aatggagtga gtatcgactg aaccgcgaag ctcatctgat ccaatccgcc
     1021 agcgcgagcc agatcctcct ttggcactag catggtgatt gaagcccgat atgccggatc
     1081 ctggaatgca ccgaagcaag agctaatagc ggtggacagg tagatgtgcc atacttccaa
     1141 cctgccggtg aagagcagtg ccaccacccc gagggtggtc accgcagcac cgctatcggc
     1201 aaggatcatg atgtgccggc ggttgtaacg gtctgcaatt gcgcctgcaa tgggggctaa
     1261 cagcaccctt ggtaaattaa aaaacaatgc gttgagggcg atgggcaatg cctgcccggt
     1321 cttttcaaaa atccacacgc tcaaaccaaa gcccgtcagg ccagatccta taatcgagat
     1381 caattgcccg aaccagatga tcaagaatgt tctcattcgt atcacgattt ggaaaccctt
     1441 cacgtctctg ctcctttggg tgcgacctag gtcgttagta tttgcttagg tatacgcccg
     1501 tgctggctcg ttgggagtta tccaatgctc ttcatctgtt gcagaaatat ctaatcgtct
     1561 tgcttgttgg gattttcaaa gtaaaaactg gggtaaaagg caatggtcag tgcatgagtc
     1621 tggacgcttt ctgagcgttc ctggttttca tatgcattga attcatccat gacctcgttc
     1681 aagcgggcaa taaacttggt ggcaagctca tctgggatgc tgcgaagttc tcggtagatg
     1741 accacctcgc gtggatgttg tttagcgccg tgatcaagcg catatgctcg tgctttcaaa
     1801 ctgcgtttga tgtctcccaa ggtcgtctca attggagcaa ccatggttgt ggttacagaa
     1861 ttttgacctt cgggggtaga aaagttgcac agtgcttcag ctagtttgaa ttcgtaagcc
     1921 gtaatccaat aatgtttctc gatgacattg gctttcactg tggtgtca
//
LOCUS       DBNHOH010000647         2624 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_38741, whole genome shotgun sequence.
ACCESSION   DBNHOH010000647 DBNHOH010000000
VERSION     DBNHOH010000647.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_38741"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="AC16V7_11290"
     CDS             complement(<1..367)
                     /locus_tag="AC16V7_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257747.1"
                     /translation="MTSAKMTSRQRVLAALKHEEPDTVPLALGGGPYGIVDDLYLRLI
                     DLLDLGAPASPFRAGHNISYMDDRLLEKLGTDIRYCWPGLMPNSPIIPGSDENTFYDS
                     YGQVWKRALPYYYSGDGILR"
     gene            489..1685
                     /locus_tag="AC16V7_11295"
     CDS             489..1685
                     /locus_tag="AC16V7_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009370445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR6257748.1"
                     /translation="MHKIAMLGTGLIGRFYTMSLLNFRGKDEIKVACAANAAHAKNFA
                     DEFGIPRWTDDMATAIRDPEIDTVIIGIPNYLHKKAALLAAEAGKAVLCTKPLATSGP
                     EALEMLEAVENAGVFHGYLEDLVYTPKTLKALDAARKGAFGKVLWARSRETHGGPHSD
                     WFWNKELSGGGAIVDMGCHCIEIARNFIGKDNRPLEVTCWADTQVHPIDAEDHAVGLV
                     RYDNGAIGQFDVSWTFRGGMDLRDEVSGTEGTVWLNHWLRTGFEMFTNVGQGGYVAEK
                     AEGDTGWLFPVGDEVGALGYVAMFLDMFDAMDTGRVPMETFYDGYIVNAIIDACYKAA
                     ETKKWEAVEIQDWRGSGLARATDIVLKDYDAERYLIKEERMPNGNLKVILKHKKTGEI
                     SQVIKE"
     gene            2132..>2624
                     /locus_tag="AC16V7_11300"
     CDS             2132..>2624
                     /locus_tag="AC16V7_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR6257749.1"
                     /translation="MSKKLFTILSALLLVTLLLGACGPKPTEAPQQPVATEEPTAPEV
                     PAAPEETAAPEEPMEEGFTIGISNPFISSEYRTQMIAELIEVNQEYMDQGITTELVIE
                     SADTDVAGQIQQLQNLIAKDVDAILVNPGDVSGLNATLEEAVAKGIIVVSVDQELNVP
                     GVYN"
BASE COUNT          718 a          595 c          695 g          616 t
ORIGIN      
        1 ccctgaggat gccatcacca gagtaataat aaggtaaagc acgtttccaa acctgtccgt
       61 aggagtcata gaaggtattt tcgtctgaac cagggatgat ggggctgttt ggcatcaaac
      121 ccggccagca gtagcggatg tccgtgccta gtttttccag caggcggtcg tccatgtatg
      181 agatgttgtg tccagcgcga aagggagatg caggtgcgcc caggtcgagt aaatcgatca
      241 ggcgtaaata gagatcgtcc acgattccat atgggccacc acccagagca agtggaacgg
      301 tatcaggctc ctcgtgtttc agagccgcga gtacgcgttg gcgagaggtc atttttgcag
      361 aggtcatcgt tgtattctca tttccttcaa aatgtcagga taaaataatt ctatcttatt
      421 gcccacaaaa ttgacgtgtg aaaattgtta ctatataatt tcctacaagt gatgaaaagg
      481 agacagctat gcacaaaatc gccatgctag ggacgggtct gatcggccgt ttttatacga
      541 tgagtttact taatttccgt ggaaaagatg agatcaaggt ggcttgtgca gccaatgcag
      601 cgcatgcgaa aaattttgcg gacgaatttg ggattccacg ttggacagat gacatggcca
      661 cagccatccg ggatcctgaa attgataccg taattattgg cattcccaat tacctgcaca
      721 aaaaagctgc cctgttggcc gccgaggctg gaaaggcggt attgtgtacc aaacctctgg
      781 cgaccagcgg gccggaagcc ctggagatgc tcgaagcagt agagaacgcg ggcgtttttc
      841 atggttatct ggaggatctg gtctacacgc ccaagacact caaggcatta gacgcagccc
      901 ggaagggtgc ttttggtaag gtactctggg cccgttcgcg cgagacacat ggcggcccac
      961 acagtgactg gttctggaac aaggagctat cgggtggcgg cgcgatcgtg gacatgggct
     1021 gtcactgcat tgagatagcg cgcaacttca ttggcaagga caaccgcccg ctcgaagtga
     1081 cctgctgggc agatacgcaa gtccatccta ttgacgctga agaccacgcg gtggggctgg
     1141 tacgttacga caacggtgcg attggccaat tcgatgtcag ctggacgttc cgcggcggca
     1201 tggatctgcg cgatgaagtt tcaggcactg agggcacggt atggttgaac cactggctac
     1261 ggacgggttt tgagatgttc acgaatgtag gccagggcgg ttacgtggcc gaaaaggcgg
     1321 aaggcgatac cggctggttg ttccctgtgg gggatgaagt tggtgctctg ggatatgtgg
     1381 ccatgttctt agacatgttc gatgcaatgg acacaggcag ggttccaatg gagactttct
     1441 acgatgggta tattgtcaat gcgatcattg atgcctgcta caaagccgct gaaaccaaaa
     1501 aatgggaagc ggttgagatc caggattggc gcggcagcgg tttggcaagg gcgactgata
     1561 tagttctcaa ggattacgat gcagagcgtt accttattaa agaagagcgt atgccaaatg
     1621 gcaacttgaa ggttatctta aagcacaaga agactggtga aatttcgcaa gtgatcaaag
     1681 aataatactg ctactggtaa agtacctgtc ttgcccatct tccttatgta agtctgataa
     1741 aacgctttgt gttttatcgc ctacttttat ctgaaaataa tcgaagctct aattattaaa
     1801 tacaaacaaa gtgattagaa ttcacaaagt aattgtacaa tacataatat tctcacatat
     1861 cgtgtaagcg caaccgactt tcgcatattc tgacctgtta tggcgtacaa tagtgctaac
     1921 tgagctaaga tacgacaagg gaggcgaagc ctgattacac tggcatgata gccctattta
     1981 cacgctctcc attttgaggt catccagaat tttggtaact ggttgagatt acgccaataa
     2041 tcagtataca cacaaggagg tagtgatatt caaagaagag aaactcaccc atcgtataaa
     2101 aatttcaata gagaatacaa gaggagaaca catgtctaaa aaattgttta ctattttgag
     2161 cgccttgctt ttggtaactc tactactcgg cgcgtgcggc cccaaaccaa ccgaggcccc
     2221 tcaacaacct gtagccactg aggagcctac agcacctgaa gtacccgcag cacctgaaga
     2281 aaccgcagca cctgaagaac ctatggaaga gggcttcacg attggcatct ccaacccatt
     2341 catcagcagc gaataccgca cacagatgat tgccgagctg atcgaagtca accaggaata
     2401 tatggatcaa ggcatcacta ctgagctggt cattgaaagc gccgacacgg acgtggcagg
     2461 ccagatccag cagttgcaga acctgattgc caaggacgtg gacgccatcc ttgtcaatcc
     2521 cggcgatgtc agcggtttga acgcaaccct tgaagaagca gttgccaaag gcatcatcgt
     2581 cgtctcggtg gaccaggaac tgaacgtgcc cggcgtgtac aatg
//
LOCUS       DBNHOH010000648         2863 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1320964, whole genome shotgun sequence.
ACCESSION   DBNHOH010000648 DBNHOH010000000
VERSION     DBNHOH010000648.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1320964"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="AC16V7_11305"
     CDS             <1..536
                     /locus_tag="AC16V7_11305"
                     /inference="COORDINATES: protein motif:HMM:NF012573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HIR6257750.1"
                     /translation="QPAAAKQLLASFTGTGGNDHYEGTSKDDVIRGLGGNDDLSGEGG
                     NDTINGGNGSDRLDGGSGADTIRGGNGKDIINGGGNNDVINGEIGNDIINGDGGNDTL
                     KGGAGNDKIRGGTGNDWIYGDEGKDNLMGNEGNDRIYGGPGTDIVNGGPGYDICIVGT
                     KETKVFECEIVYRWANQ"
     gene            complement(540..1451)
                     /locus_tag="AC16V7_11310"
     CDS             complement(540..1451)
                     /locus_tag="AC16V7_11310"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR6257751.1"
                     /translation="MSFEKSRPHTARNKRILKLTAFTALSCALLYLIISTSLAGVYAY
                     FLTHPACMTNPPRIPGLPSPEEHWLSTSDGLSLRTWYYPGDNGAAILALGGMGGALGE
                     NLPPVEFLIHAGFGVLQIDSRACARPASVVTLGAKEVLDAEAGLSFLRNRPEVKKIGI
                     FGFSMGAATAVRTAARQVDIAAVVAEGGYFNLGEDFVEPESQINLLYRTFLYTIAGAY
                     QFFSSVNPWEVSPIDDLPSISPRPLLLIYGEGEAGSGRALAQFDAARKPKTLWIVPGG
                     AHGSNFAASPGEYQSRIVEFFNETLQP"
     gene            complement(1441..2373)
                     /locus_tag="AC16V7_11315"
     CDS             complement(1441..2373)
                     /locus_tag="AC16V7_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR6257752.1"
                     /translation="MYEPAYVKLLNNNELAQRVSYAYDMLSKCNVCAWECRIDRLVGK
                     VGICKTAQRAKVSSYGPHLGEEDPLRGWRGSGTIFFTRCNLRCQFCQNHDISQTDKGE
                     EIEPGQLAAIMLELQSLGCHNINLVSPSHVVPQIMAAVLIAAQAGLRIPLVYNTGGYD
                     SVEMLKLLDGVVDIYMPDMKYASAQIARYYSKIPNYPQANQAAVREMHRQVGDLHLDQ
                     NGLATRGLLVRHLVLPHNLAGTETVVQFLAEQISTDTYLNLMDQYRPAYNAQRFPKLK
                     RRTTQQEYQAAVDMAHAAGLHRLDKRKPLNWHVI"
     gene            2568..>2863
                     /locus_tag="AC16V7_11320"
     CDS             2568..>2863
                     /locus_tag="AC16V7_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257753.1"
                     /translation="MLGANNALEASAIDDADPQFQDFYRRLGGRQILGPVISRLFTNG
                     QISYQYTVAGLMAYDPLASEHERYYLAPLGLDLGILEAVVQPPDDPSLRYVDGH"
BASE COUNT          667 a          698 c          792 g          706 t
ORIGIN      
        1 ttcagccggc agctgcgaaa cagctccttg caagcttcac cggcacgggg ggtaatgatc
       61 attacgaagg cacatccaag gatgatgtta ttcgtgggtt gggtggcaac gatgacctga
      121 gcggtgaggg cggcaacgat accatcaatg gcggcaatgg gagcgacagg ttagacggtg
      181 gcagcggtgc cgataccatc cgaggcggca acggaaagga catcatcaat ggtggcggca
      241 acaatgatgt tatcaatggc gagatcggca atgacatcat caatggcgat ggtggaaacg
      301 atactctcaa agggggtgca ggtaatgata aaatccgtgg cggcaccggc aatgactgga
      361 tctacggtga tgaaggaaaa gataacttga tgggcaatga aggcaacgac cgtatatacg
      421 gtggccctgg cacggacatt gtgaatggcg gccctggcta cgatatttgc attgtgggca
      481 ccaaggagac taaggtgttc gaatgcgaga tcgtttatcg ttgggctaac cagtaattac
      541 tatggttgta aggtttcgtt gaaaaattca acgattcgac tttgatactc acccggcgag
      601 gcggcgaaat ttgaaccgtg cgccccgccg gggacaatcc aaagggtttt tggctttctg
      661 gcagcatcga attgggccag ggcgcgtccg ctacccgctt ccccctctcc ataaattaag
      721 agcaatggtc ttgggctgat cgatggcaga tcgtcgattg ggctcacttc ccagggattt
      781 acactgctga aaaactgata agccccggcg atggtgtaaa gaaatgtccg gtatagaagg
      841 tttatttggc tttcaggctc gacgaaatcc tctcccagat taaagtaccc cccttcagcg
      901 accactgcgg cgatatctac ctggcgagca gcagtgcgca cggcggtagc ggcacccatc
      961 gaaaatccaa aaattccaat tttcttcacc tctgggcggt ttcggaggaa tgatagtcct
     1021 gcctcggcgt ctaagacctc ttttgcccct aatgtgacca cagaggcagg acgggcacag
     1081 gcacggctgt cgatctgaag tacgccgaaa cccgcatgga tcaagaattc caccggtggc
     1141 agattctcgc ccaacgcgcc gcccatcccg cctagggcga ggatggcagc cccattatcc
     1201 cctgggtagt accaggtgcg caggcttagg ccatctgatg tgctcaacca gtgttcttcg
     1261 ggagaaggca ggcctggaat gcgtggtggg ttggtcatgc aggcagggtg tgtcaggaag
     1321 taggcgtaca ccccggccag actggtactg atgatcaaat ataacagggc acaggataac
     1381 gctgtgaagg ctgtcagctt tagtattcgc ttgtttcggg ccgtgtgggg tcggctcttt
     1441 tcaaatgaca tgccagttca gcggtttgcg tttgtccaac cgatgtagcc ccgcggcatg
     1501 tgccatatcc actgcagcct gatattcctg ctgggtggtg cggcgtttaa gcttggggaa
     1561 tcgctgagca ttgtatgctg gacggtattg gtccatcaga ttcaggtacg tatcggtaga
     1621 gatttgctct gccaggaatt gcacgacagt ctcggtgcca gccaggttat gagggagcac
     1681 taggtggcga accaataaac cccgggttgc gagaccgttc tggtccaggt gcaagtcacc
     1741 tacctggcgg tgcatctcgc gcacagcagc ctgattggct tggggataat tgggaatctt
     1801 ggaataatag cgagcgatct gcgcactagc gtatttcata tctggcatgt aaatatcgac
     1861 cacaccatcc aatagcttta gcatttcgac ggaatcatag ccaccggtgt tgtagacgag
     1921 agggatacgc aacccagcct gagcagcgat gagcaccgca gccatgattt gtggtacaac
     1981 atggctgggt gagaccagat tgatattgtg gcaacccagg gattgtaatt ccagcatgat
     2041 ggctgccagt tgccccggtt ctatctcttc ccctttgtcg gtttggctga tatcatggtt
     2101 ctggcagaat tgacagcgca ggttgcagcg cgtgaagaaa atagttcctg acccacgcca
     2161 accacgcaat ggatcttcct cacctagatg cggaccatag ctgctgacct tggcgcgctg
     2221 ggcagttttg cagatgccca ctttcccgac taggcgatct atcctacact cccaggcgca
     2281 tacgttgcat ttggagagca tatcataagc atacgaaacg cgctgcgcca gttcattgtt
     2341 gttcagtagt ttgacataag ccggttcgta catcaatatg cctgattatt ttctattgaa
     2401 atcttatagc ttctggcaaa ttgcctggtt tctgactata attttgatgc tgccagttcc
     2461 ttcaagtatt tcactgcttt ggcttcatat tattttacat gcattttaaa caggcgaacg
     2521 ttctaatcgt tcttttcatt ttcgtaatct caacctctgc atgcggaatg ttaggggcaa
     2581 acaatgcatt agaagcatcc gctatcgatg atgccgatcc ccaatttcag gatttttacc
     2641 ggcgcttggg cggacgtcag atattgggac cggtgatttc acgcttgttt acgaacggtc
     2701 aaatcagcta ccaatataca gtggcagggt tgatggctta tgatccgctg gcgtctgaac
     2761 atgaacggta ttatctggcg ccgcttggct tggaccttgg catccttgag gcagtggtcc
     2821 aacctcccga cgatccttcc ttacggtatg tggacgggca tat
//
LOCUS       DBNHOH010000649         1446 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1127979, whole genome shotgun sequence.
ACCESSION   DBNHOH010000649 DBNHOH010000000
VERSION     DBNHOH010000649.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1446)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1127979"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..950
                     /locus_tag="AC16V7_11325"
     CDS             39..950
                     /locus_tag="AC16V7_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HIR6257754.1"
                     /translation="MTASKYMTLEEAISTFVLDGSSLALGLAQETLIPFAAGWEIMRQ
                     SRRDLTLIGPISDILFDQMIGAGCVRWVRSAWVGNVITGSGYNFRRAVESGTLEIEDY
                     SNLSLAMALQAGALGVPFLPTYTALGSDLFQTNPSLNKVVCPFTGQVLTAVAALKPDV
                     AIVHVQRSDEQGNAHAWGNLGMTREACLAAEQVILTCEEIVPAEVISSDPNRVVVPGF
                     RVVAVVQAPWGAYPSPVPGYYNRDHQAFLEYQEASHTAERFASWLESRVVAPKDYDSY
                     LELIGKTRLAELVPKVSAPSAVVDFGY"
     gene            1002..>1446
                     /locus_tag="AC16V7_11330"
     CDS             1002..>1446
                     /locus_tag="AC16V7_11330"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HIR6257755.1"
                     /translation="MLVHSERYTAAELMITAAANEIVNGEVVFVGMRLPLLGFQLAKS
                     THAPHASGIFELGIVRDKPAPEPILTMGDLPNLYHAQWLTDTMGLMGMLQRGMVDVSF
                     IGGAQVDRFGNLNTSYIGPVCQPQIRLPGSGGACDLASLAKRHIIV"
BASE COUNT          311 a          353 c          416 g          366 t
ORIGIN      
        1 agtcccaagt gcaattaatc tctggacgag gagggtctat gactgcgtcg aaatacatga
       61 ctctggaaga agcgatcagc acctttgtat tggatggttc atcgttggcg ctgggtctcg
      121 cccaggagac gcttatcccc tttgctgccg gttgggaaat catgcgccaa tcccggaggg
      181 atctcacatt aatcggcccg atttcggata ttctttttga ccagatgatc ggtgctggtt
      241 gtgttcgctg ggttcgttct gcctgggtgg gaaatgtcat tacgggctca ggatacaatt
      301 tccgacgagc agttgagagc ggtaccttgg agattgagga ttattccaac ctgtcattgg
      361 ctatggcctt gcaagccggt gcgctgggtg tgcctttcct ccctacctac actgcactgg
      421 gtagtgactt attccaaact aatcccagcc tgaacaaggt cgtttgtcct tttaccggtc
      481 aggtcctgac ggccgtggca gcactcaagc cggatgtggc catcgtccac gtgcagagat
      541 ctgacgagca aggcaacgct catgcctggg gtaacctggg catgactcgc gaagcctgtt
      601 tagctgccga gcaggtcatc ttgacttgcg aagagatcgt acccgccgaa gtgatcagta
      661 gcgatccaaa ccgcgtagtg gttcctggtt tccgggttgt tgcagttgtt caggcgccct
      721 ggggcgctta tccatcaccg gttcccggtt actataaccg agatcaccag gctttcctcg
      781 aataccagga agcaagccac accgcagagc gttttgccag ctggctggag agtcgggtgg
      841 tcgcgccaaa ggattatgat agctatctgg agctgattgg gaaaacacgc ttggctgagc
      901 ttgtgcccaa agtaagtgcg ccctcggcag tggtggactt tggatattga ttgcccgaag
      961 acaggcatta tcagaccaga tcagagcagg cgaggatgat catgctagta cattctgagc
     1021 gttacacagc cgctgaatta atgatcacag cagctgcaaa tgaaattgtc aatggggaag
     1081 tggtctttgt cggaatgcgc ttaccgttat taggcttcca attggcgaag tccacccatg
     1141 ccccgcatgc cagtgggatt tttgaattag gtattgtccg cgataaaccg gctccggagc
     1201 ctattctcac aatgggggat ttgcctaatc tataccacgc gcagtggctc acggatacta
     1261 tgggattaat ggggatgctc cagcgaggga tggttgacgt gagctttatc ggcggcgctc
     1321 aggtggatcg ttttggcaat ttgaatacca gctacattgg accagtgtgc caaccacaga
     1381 tccgcttgcc gggtagcggt ggggcttgcg atctggcttc actggctaag cggcatatta
     1441 ttgtga
//
LOCUS       DBNHOH010000650         1947 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_166155, whole genome shotgun sequence.
ACCESSION   DBNHOH010000650 DBNHOH010000000
VERSION     DBNHOH010000650.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_166155"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="AC16V7_11335"
     CDS             complement(<1..275)
                     /locus_tag="AC16V7_11335"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HIR6257756.1"
                     /translation="MDQLLIVFIIFLAVFTQSLAGFGSALVAMAFLPELLGIRVATPL
                     VAMVAMTLEGILLVRYRAVLNLRAVWQVSVASLVGIPLGMLTLKRID"
     gene            complement(426..1943)
                     /locus_tag="AC16V7_11340"
     CDS             complement(426..1943)
                     /locus_tag="AC16V7_11340"
                     /EC_number="4.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016841 - ammonia-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0170035 - L-amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAL/PAL/TAL family ammonia-lyase"
                     /protein_id="HIR6257757.1"
                     /translation="MVIDGSDLTVEKMVRIARDGEQVELYPEALGRIKACRGMLEEKL
                     KAREIMYGTNTGIGEFSEVVLTDEQVEQFQRYLIYNHAAGIGEPVSIENVRGAIASRI
                     NVHAHGMSGCRPEITLTLVDMLNKGVTPVVCQKGSVGACGDLAPMAQIALLLMGEGEA
                     YFQGERLPGALAMERAGIPVPGLQARDGLATINGSNLITAISALHLYDMNRWLKQAEI
                     ACAMSLEALKANLKPYDVRLHQARGFSGAVRSAKAIMQCIAGSDLVTGKMKTKVQDAY
                     SMRSSPQVIGAAHDAVAFARSQVEIELNGVGDNPIFMPEYNLTLTGANFQGSPVSLPM
                     DMAGAAITMVCVLSERRMNRLTNPALSVGLPAFLTKGAGMFSGLMLSQYTADSLIVEQ
                     RILSMPASIQSIPAAADQEDFVSMGMNTALKNNQILDNAYGVLGIEFMAAAQALDFRE
                     FTPGHGVQVARQVIRKHVAYLDEDRPLYPDHNAMKALVQSCEILQAVEDEVGSLG"
BASE COUNT          444 a          598 c          502 g          403 t
ORIGIN      
        1 tcatcgatgc gtttgagggt cagcatgccc agcgggatgc ccaccagaga tgcaactgac
       61 acctgccaca ccgctcgtag attcagcact gcgcggtagc gcaccaacaa gataccctcc
      121 aaagtcatgg ctaccatcgc taccagagga gtagcgacgc gtatgcccaa taattctggc
      181 aaaaaagcca tagccaccaa cgccgagcca aatcccgcca ggctctgtgt gaatacagcc
      241 aaaaagatga tgaaaacgat gagaagctga tccatatctc ccaattaacg ggacaattct
      301 aaccgatcgg ctagaattcg caatcccttt actatattcg ctcccagaca atgacaggca
      361 ccctcaagca ataaaaatcc cccttcgcct agaaggggga tcacaaatat tgatcactgg
      421 aggctttatc ccaaactacc cacctcgtct tcaaccgcct gcaaaatctc acacgactgc
      481 accaaagctt tcatcgcatt gtggtccggg taaagaggcc ggtcttcatc caggtaggcg
      541 acatgcttgc ggatcacctg gcgagctacc tgcactccat gcccgggagt gaactcacgg
      601 aaatccaagg cttgggcggc agccatgaac tcaatgccca gcaccccgta ggcattatcc
      661 aatatctgat tattttttaa ggcagtgttc atgcccatag agacgaaatc ctcctggtct
      721 gctgctgccg gtatggattg gatgctggct ggcatggaga gaatgcgctg ctccacgatc
      781 aagctatcgg cggtgtactg gctgagcatc agcccagaga acatgcccgc tcccttggtc
      841 aggaaagccg gcaggccgac gctgagagcc gggttggtca ggcggttcat gcgtcgctcg
      901 gacaacacac acaccatggt gatggccgcg cctgccatgt ccattggcaa tgaaaccggc
      961 gaaccctgga aattcgcccc ggttaatgtc agattgtact ccggcatgaa gatggggtta
     1021 tcgccgactc cattcagctc aatttcaact tgactgcggg cgaaggcgac tgcgtcgtgc
     1081 gccgcgccga tcacctgggg tgaagagcgc atggagtagg catcctgtac cttggtcttc
     1141 atcttgccgg tcaccagatc gctccctgca atgcactgca tgatagcctt ggcgctgcgc
     1201 acggctcctg aaaagccacg cgcctggtgc aggcgaacgt cataaggctt caagttggct
     1261 ttcagggctt ccaggctcat ggcgcaagcg atctcagcct gcttcagcca acggttcatg
     1321 tcgtacaggt gcaaagcact aatggcagtg atcaagttgg agccattgat ggttgccagc
     1381 ccatcccgcg cctgtaagcc gggaactggg atacccgccc gctccatagc cagggcgcct
     1441 ggaagccgct caccctggaa ataagcttcg ccctcaccca tcagcaacag ggctatctgt
     1501 gccataggtg ccaggtcgcc acacgccccc accgacccct tctggcacac caccggcgtc
     1561 acccccttgt tgagcatgtc caccagggtc agggtgattt caggacggca gcccgacatg
     1621 ccgtgggcgt gcacattaat gcggctggcg atagcaccgc gcacgttttc aatggagact
     1681 ggctcgccga tacccgcggc gtggttgtag atcaggtatc gttggaactg ctctacctgc
     1741 tcgtcggtga gcaccacctc ggagaactca ccgatgccgg tgttcgtgcc gtacatgatc
     1801 tccctcgcct tgagcttctc ctccagcatg ccacggcagg ctttaatacg acccagcgct
     1861 tcggggtaca actccacttg ctcgccatct ctggcgatgc gtaccatttt ctcaactgtc
     1921 agatcggaac cgtctataac gatcgtc
//
LOCUS       DBNHOH010000651         2503 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_295622, whole genome shotgun sequence.
ACCESSION   DBNHOH010000651 DBNHOH010000000
VERSION     DBNHOH010000651.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_295622"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /gene="ygfZ"
                     /locus_tag="AC16V7_11345"
     CDS             complement(<1..979)
                     /gene="ygfZ"
                     /locus_tag="AC16V7_11345"
                     /inference="COORDINATES: protein motif:HMM:NF013720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CAF17-like 4Fe-4S cluster assembly/insertion
                     protein YgfZ"
                     /protein_id="HIR6257758.1"
                     /translation="MGTSELTGYQAAISSAVFYLQPKAGYLKVEGTDRIAFLQRQTTN
                     DLTQLALKGCLTSILTSPSARILDMFRVIDEGDSLGIITLAGHAESTLRYLKSRIFFM
                     DKVSVVDLSVEFAQIDLEGPGATKWLTELGIGIAPKLDEIVSVDMDGLPLRILGQPGL
                     SGQGYRLLVPAAAQPSLENTLNGLGIAHLEEEAYQALRVEAGLPAPGTELTEDYTPME
                     IGLDWAISDKKGCYTGQEVIARQITYDKITQQLVGLQLSSPAQPGERVWSDVKPAGKV
                     TSFARSPRFGEIALAVLKKPFHLPNTEVFVGSAPDLSSSAIVSVLPINSK"
     gene            complement(1048..>2503)
                     /gene="ftsH"
                     /locus_tag="AC16V7_11350"
     CDS             complement(1048..>2503)
                     /gene="ftsH"
                     /locus_tag="AC16V7_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR6257759.1"
                     /translation="QGSNNAAMSFGKSRARMFTGDQPSVTFEDVAGVDEAKEELEEVV
                     EFLREPEKFISLGARIPKGVLLVGPPGTGKTLLAKAVSGEAGVPFFSISGSEFVEMFV
                     GVGASRVRDLFDQAKRHSPCIVFVDEIDAVGRQRGAGLGGSHDEREQTLNQMLVEMDG
                     FDTDTNVIIVAATNRPDILDPALLRPGRFDRRVILDRPDMRGREEILKVHVKGKPLST
                     DVELGVLARSTPGFVGADLENLVNEAAILAARRNKKMIGQPEMEESIERVIAGPERKS
                     RLISPEEKRIVAYHEAGHAVVMNALPEADPVHKVSIIARGMAGGYTLALPEEDRTLMP
                     RKKMIADMIGLLGGRAAEELVFDDITSGASNDLERVTRMARAMVTRLGMSNNLGPMVY
                     GQKEELIFLGREISEQRDYSEAVAEEIDDEVRRLVSEAYEKSKAILIEYRAKLDAVAH
                     RLLEVETITRDEFESIFPAPVPKNSGTPVPQAVM"
BASE COUNT          550 a          664 c          663 g          626 t
ORIGIN      
        1 atttggagtt tattggcaat actgagacaa ttgctgagga tgataaatca ggcgcgcttc
       61 cgacgaatac ttccgtgttg ggtaggtgaa aaggcttttt tagtacagca agggcaatct
      121 cgccaaatct tggtgagcgc gcgaaggagg ttaccttgcc agctggtttt acatctgacc
      181 atactcgttc tcctggttgg gcaggtgaac tcagttgaag accaaccagt tgttgggtga
      241 tcttatcgta tgtgatctga cgggcgatta cttcctgacc agtgtaacac ccctttttat
      301 cggagatagc ccaatccaag ccgatctcca ttggggtgta gtcctcagtc agctcagttc
      361 caggtgcggg caggccagct tccacgcgca atgcctgata tgcttcttct tccaggtgtg
      421 cgattcctaa tccattgaga gtgttttcca aagatggttg tgccgccgca ggtacgagaa
      481 ggcgatatcc ttggccgctt aagccgggct gtcccaggat gcgcaggggg agcccgtcca
      541 tatctacgct aacaatctca tccaatttag gggcaatccc aatgcctagc tcagtaagcc
      601 atttggttgc gcccggtccc tccagatcta tttgggcgaa ttcgacactg aggtcaacca
      661 cactgacctt atccatgaag aagatacggc tttttagata gcgaagcgtg gactctgcgt
      721 gtcctgcgag ggtgatgatt cctaaggaat ctccttcgtc aataacgcga aacatatcca
      781 ggatgcgcgc actgggggag gttagtatgc tggtgaggca acccttcaag gcgagttgtg
      841 tcaggtcgtt ggtagtctgc cgctgtaaaa aagcaatacg atcagttcct tcaaccttca
      901 gatagcctgc tttgggctgg agatagaaca ctgcgcttga aattgccgcc tggtaaccag
      961 taagttcgga ggtacccatt gtagatggtg gtaagtggag ttaatagctg atacgggtgc
     1021 tggagcaccc gtatcactaa ttgtttgtta catgaccgct tgaggcactg gcgtgccgct
     1081 gttctttggc acgggtgcag ggaagatgct ctcaaattca tcgcgggtta tggtctcgac
     1141 ttccaacaag cgatgcgcta cggcatccaa ctttgcgcga tactcaatga gaatggcttt
     1201 ggatttctcg taggcttcgc ttaccagccg acgcacttca tcatcgatct cctctgcgac
     1261 cgcttcagaa taatcgcgct gttctgaaat ctcgcggcca aggaaaatca gctcttcttt
     1321 ctgaccatag accatcggtc ccaagttatt actcatgccc agacgggtaa ccatggcgcg
     1381 tgccatgcgg gttactcttt ccaggtcgtt tgacgctcca gacgtgatgt catcaaatac
     1441 cagctcttcg gcggcgcggc cacctaagag gcctatcatg tctgcgatca ttttcttgcg
     1501 cggcattagc gtcctatctt cctcgggtaa ggctagtgta taacctccag ccatgccgcg
     1561 ggcaatgatt gacaccttat gcacggggtc agcttctggc aatgcgttca ttactaccgc
     1621 gtgacctgct tcatggtagg ctacgatgcg cttctcttcc gggctaatca atcgactctt
     1681 gcgctccggt ccagcgataa cacgctcaat ggattcttcc atctcaggct ggccgatcat
     1741 tttcttgttc cgccgggcgg ctaagatggc agcttcattg actagattct ccaggtcagc
     1801 acccacaaat ccgggtgtag agcgggctaa aacacccaat tcaacatccg ttgagagtgg
     1861 tttgcccttg acatgtacct tgagaatttc ctcgcgcccg cgcatatctg ggcggtcgag
     1921 gatcactcgc cggtcaaagc gtccaggacg cagcagagca ggatcgagga tatcgggccg
     1981 gttggttgcg gcgacaatga tcacattggt gtcggtgtcg aagccatcca tctcaaccag
     2041 catctggtta agggtttgct cgcgctcgtc gtggctgcca cccagaccgg cgccgcgctg
     2101 gcgaccgact gcatcaattt cgtccacaaa gacgatgcat ggggaatggc gtttggcttg
     2161 gtcgaacaag tcgcggacgc ggcttgcgcc tacaccaacg aacatttcaa cgaactcaga
     2221 gccagaaatt gagaagaaag gtacgccggc ttcaccggag accgcttttg cgagcagagt
     2281 cttaccggta ccgggcggac cgactaggag cacacctttc gggatgcgtg cccctaaaga
     2341 gatgaatttc tcgggttcgc gcagaaattc aacaacttct tccaattcct ctttcgcttc
     2401 atcgacacca gcgacatcct caaatgtaac cgagggttgg tcgccggtga acatgcgggc
     2461 tcgggacttt ccaaacgaca ttgcggcgtt attactgccc tgc
//
LOCUS       DBNHOH010000652         1140 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_39023, whole genome shotgun sequence.
ACCESSION   DBNHOH010000652 DBNHOH010000000
VERSION     DBNHOH010000652.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_39023"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="AC16V7_11355"
     CDS             complement(<1..213)
                     /locus_tag="AC16V7_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257760.1"
                     /translation="MKKIFTIGMALVAALMVFSTVALADHNSPGYSYTTDQINLGNAE
                     SARYQGLADHFAAKNVATQRALEATSA"
     gene            complement(514..>1140)
                     /locus_tag="AC16V7_11360"
     CDS             complement(514..>1140)
                     /locus_tag="AC16V7_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6257761.1"
                     /translation="AIVRDGLVMLLQAQPDLEVIGTADNGREAVRQIKQLQPDVAILD
                     ITMPDLNGLEAARQIREAGLDTRVIILSMHGSTSHVFEALKVGVKGYLLKASAGAMVV
                     DAVRAVYRGQRYLSDEIMEQVIDHSLLQQSEQQDKTPDPLASLSPREREVLQLVVEGK
                     TSAEIAQMIHISPKTVETYRGNLMKKLGLHNPTELVKFAIRYGLTPLE"
BASE COUNT          220 a          323 c          326 g          271 t
ORIGIN      
        1 ggcggacgtg gcttctagag ctcgctgggt tgctacattc ttggcagcga agtgatcagc
       61 cagaccctgg tagcgggcag actcagcatt gcccaagttg atttgatccg tagtgtatga
      121 atagcccggc gagttatgat ccgccagcgc tacagtgctg aagaccatca gggctgccac
      181 cagcgccatc cctatagtaa atatcttttt catattatta tcctcactta cttttctaaa
      241 tattttgggg tcttcagctg gaaaattgcc cctgcgatct aaccaagaac tgacatgccc
      301 caccaattga tgttattatg gcagattgtg cacctcttgc atatcgggaa tgtcggagac
      361 tcgccatggg gaaaacacct ctttacctcc cctttatggc ggcgtgcagc cgaaaggcaa
      421 aggcctggtt tccggcacgg gggtacggat tccttggccc cgctgtgctc cggcccggga
      481 tgattggttc agcagaactc gggtgacaga atgctactcg agtggggtca agccatagcg
      541 gatggcgaac ttgaccagct cggtcgggtt gtggagcccc agttttttca tcaagttgcc
      601 gcgataggtt tcgaccgtct tgggagaaat atggatcatt tgggcgatct cggcgctcgt
      661 tttaccctca accaccagct gcagcacttc gcgctcgcgc gggctgaggc tggcgagagg
      721 atccggagtc ttgtcttgct gctctgattg ctggagcagg gaatggtcga tcacctgctc
      781 cataatctcg tcgctcaggt atcgttgacc gcggtagact gcgcgcaccg cgtcgacgac
      841 catggcgccg gccgaggcct tcagcaggta gcctttgacc cccaccttca gggcttcgaa
      901 gacgtggctg gtggagccgt gcatcgataa gatgatgacc cgcgtatcca ggccggcctc
      961 ccggatttgc cttgcggcct ccagcccgtt caggtccggc atggtgatat ccaggatggc
     1021 cacatctggc tgcagctgct tgatttggcg caccgcctcc cggccgttgt cggcggtgcc
     1081 gataacctcc aggtcgggtt gcgcttgcaa cagcataacc aatccatcgc gcacaatggc
//
LOCUS       DBNHOH010000653         4776 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1385579, whole genome shotgun sequence.
ACCESSION   DBNHOH010000653 DBNHOH010000000
VERSION     DBNHOH010000653.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1385579"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..2210)
                     /locus_tag="AC16V7_11365"
     CDS             complement(141..2210)
                     /locus_tag="AC16V7_11365"
                     /inference="COORDINATES: protein motif:HMM:NF042769.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257762.1"
                     /translation="MRRISAITRTAVVMMLFTWLSACSSQPPTPAKSPPTHTDIPVEP
                     TAAPVTPEARAVATQTLPEPTAPPSWDYVAIGGDFTYAQNWDKLYAGYMEDDLEVPVN
                     FSDLSTHDFMTWNGWLERLKNEEQLRSALQGAEAVTFDVPLEAYFSMPIAMYQNKQCG
                     GDDGENCYRQRVEQLRQDLDAFLAELTALADPSQVVVRTVLRGSIPAFYGEGVGEGFV
                     QDFSEAEMQALIKYMLAANEVVRQAAAAHGIPAVDLPATLQPDGPDTPPASEYLLMLG
                     LSEAGDQEVADLLRQAGYESSPQAEVTLREEGCSLGVYANPVPNPIQVKVANPTNEIM
                     ALVVVTLKEDASRYDLLEFTGGGIPDEVDKRVAHPEPGPNNEMITSITLEEGRENYFV
                     CAQPEKVLAVPGALTPGQAYQPFPPVRAVREVPYTKPVSATAQEFKLDIYAPKQAGLY
                     PVVVFLHGGNTSKDDPVYQELFTDLVMAGAVVLSAQSFEDSGGALARADKGAGIRHDH
                     ENAACAVRYARAVAPQYSGDPKQVILIGHSGGGYTGLWVTLAGDQLDQAWETVASQGG
                     PPRQVECLAGQDESPLPDGFIGYNGGLVYFDQMRNEFPSLFKVLDPRSHVGRNPEIPL
                     LLVFGDQDFSYVYMPWFKKGVDEMVSMLEKAGHEVQFELWPGIGHSVDSRSIPLIVSA
                     FQRMVRR"
     gene            2307..3467
                     /locus_tag="AC16V7_11370"
     CDS             2307..3467
                     /locus_tag="AC16V7_11370"
                     /inference="COORDINATES: protein motif:HMM:NF012742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR6257763.1"
                     /translation="MKFVTNGSVMAPLPIVIQPASDLPLAFQLKQQITWLVATNVIKP
                     GEKLPPIRVLAAQVGVHYQTVRAVYHQLSEDGLVAIRQGSGATVLPFSGLQLARPETS
                     VHAKMVGVLIAGMDPFYLPFLQGVEEVAEALPSLVVICNAEDNPVKAKLQINQLILRG
                     MDGIIAASVGNLVQRELGFPQTGGPFPIVYCDQPDTSGHVILFDAENAGYQAVKHLLD
                     HGHRRIGIVTAPIDWPNMSECFAGYRRALMEAGIAPRQSLVSVTKGFSISQGSDAARS
                     LLVGSNPPTAMFATADILAIGAQRAIKSEGLRVPEDVAMIGYNDIEIASMVEPALTSL
                     AAPAYEMGRLAMGLLARLIAGERVNPTITRLETSLVIRRSCGCYPPGEGDHH"
     gene            complement(3515..4279)
                     /locus_tag="AC16V7_11375"
     CDS             complement(3515..4279)
                     /locus_tag="AC16V7_11375"
                     /inference="COORDINATES: protein motif:HMM:NF015722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HIR6257764.1"
                     /translation="MWGRSASLLAIALLGLAGCSRPTPTPTIPKSDLVQVPAGWFWMG
                     EDDARRSSQPQHRVYLDTFEIQRTEVTRSDYIQYLAAIGYPAPDTDQSKLEAYSQLPV
                     VGILWENADAYCRWIGMRLPTEAEWEKAARGDNGRHFPWGNEWDPNKANTSENGQGNV
                     LPVGSFLDGASPYGLLDMCGNAAEWVADYYQASYYHDAPDRNPTGPTQVTDHVLRGGS
                     FDSTYEQTTTYFRDSSHSVRPNLRVGFRCARSVETK"
     gene            complement(4381..>4776)
                     /locus_tag="AC16V7_11380"
     CDS             complement(4381..>4776)
                     /locus_tag="AC16V7_11380"
                     /inference="COORDINATES: protein motif:HMM:NF013381.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HIR6257765.1"
                     /translation="YIAFIKAHTKAKVFFHTDGDVFDLIDDFIEIGVDILNPIQTSAG
                     KMVDLVAIKKRYGRNLCLCGAVDTYRIVPFGTPEQVRQEVRRVIDILGPGGGYMVAAV
                     HTILDEVPPENILAMVDAVEEFGHYPLGH"
BASE COUNT          970 a         1374 c         1363 g         1069 t
ORIGIN      
        1 tcaagatccc atccgagttc ttgctgatgg taccctatgt cgccacgatg gtcgtgctgg
       61 cgggcgtggt cgggcgcagc cgtatgccag ctgcagatgg caagccgtat gagaaagaag
      121 ggcactaagg atttgattgc ctagcgcctc accatcctct ggaacgcaga cacgatgagt
      181 ggtatgctac ggctatcgac agagtgaccg atgcccggcc acaactcgaa ctggacttca
      241 tggccggctt tctcgagcat ggataccatc tcgtccaccc cttttttgaa ccagggcata
      301 tatacataac tgaaatcctg gtcgccgaac accagcagga gaggtatttc aggattgcga
      361 cccacatggc tgcgcgggtc tagtaccttg aataaggagg gaaattcatt tctcatctgg
      421 tcgaaataga caaggccacc gttgtatccg atgaagccat ctggtagagg gctttcatcc
      481 tgccctgcta ggcattccac ctggcggggt ggcccgccct ggcttgcaac agtttcccag
      541 gcctggtcga gctggtcgcc tgccagggtg acccacaaac cggtgtagcc tcccccggag
      601 tggccgatca ggataacctg cttagggtcg ccgctgtatt gcggcgcaac ggcgcgggca
      661 tagcgaaccg cgcatgccgc gttttcgtgg tcatgacgga tgccagcacc cttatctgcg
      721 cgggcgaggg ccccgcctga atcctcaaat gactgggcag agagcaccac tgctcctgcc
      781 atcacaaggt ctgtgaacag ctcctggtaa accgggtcgt ctttggaggt gttgccaccg
      841 tgtaggaaga ccaccaccgg gtataatcct gcctgtttcg gggcgtagat gtccagctta
      901 aattcctgcg cagtggctga gacaggcttg gtgtagggca cttcccttac cgcgcgcacc
      961 ggcgggaagg gttggtaggc ctgcccgggg gtcagcgccc ccggcaccgc cagcactttt
     1021 tccggctggg cgcagacgaa gtaattctca cgcccctctt ccagggtgat gctggtaatc
     1081 atctcgttgt tcgggccggg ctccgggtgg gcgacgcgct tatcaacttc atccgggatc
     1141 ccgccgccag tgaattcgag cagatcgtag cggctggcgt cctctttcaa cgttaccacc
     1201 accagcgcca ttatctcatt ggtcgggttg gcgaccttca cctggatcgg attcggcacc
     1261 gggttggcgt atacccccag gctgcagccc tcctccctta aggtcacctc cgcctggggt
     1321 gaggattcgt agcccgcctg gcgcagcaga tcagccacct cctggtcccc ggcctcggac
     1381 aggccaagca tgagcagata ttccgacgcc ggaggggtgt ccggcccgtc cggctgcagg
     1441 gtggccggca ggtcaaccgc agggatgccg tgcgcagctg ccgcctgacg caccacctca
     1501 ttggcagcca gcatgtactt gatcagcgcc tgcatctccg cctcgctgaa atcctgcacg
     1561 aagccttcgc cgaccccctc gccgtagaaa gcggggatcg aaccgcgcag caccgtgcgc
     1621 accaccacct ggctgggatc ggccagcgca gtcagctccg ccagaaaggc atccagatcc
     1681 tggcgcagtt gctcgacgcg ctgccggtag cagttctccc cgtcatcgcc gccgcactgc
     1741 ttgttctggt acatggcgat gggcatcgag aaataggcct ccaggggcac atcaaaagtg
     1801 accgcctccg ccccctgcag ggcgctgcgc agctgttctt cgtttttcag ccgctccagc
     1861 cagccgttcc aggtcatgaa gtcgtgggtg gacaggtcgg aaaagttgac cggcacttct
     1921 aggtcatctt ccatgtaacc ggcgtacagc ttgtcccagt tctgcgcgta ggtgaaatcg
     1981 ccgccgatgg cgacatagtc ccatgatgga ggcgcggttg gctccggcag agtctgggtg
     2041 gctacggcac gcgcttccgg ggtgactggt gccgcggtgg gctccacggg tatatcggtg
     2101 tgggtggggg gggactttgc tggggtgggc ggctggctgc tgcacgcgct cagccaagtg
     2161 aagagcatca tgacaacggc agtgcgggtg attgcggata tacggcgcat cagtccctcc
     2221 taggttggat cagcaggccg gtacgatcaa tgtgttataa tggattataa cacattgatt
     2281 tatttcatgt caagcaggta gaattaatga aatttgtcac aaacggttcg gtgatggctc
     2341 ctctccccat tgtcatccaa ccagcctcag atttacccct ggcgttccag ctcaaacagc
     2401 aaatcacctg gctggtcgcc actaacgtga tcaaacctgg cgaaaagctc ccacccattc
     2461 gggtgctagc agcccaggtg ggtgtgcatt accaaaccgt gcgggcagtc tatcaccagc
     2521 tctcggaaga cggcttggtg gccatccgcc agggatcggg tgctaccgtg ctgccattct
     2581 ccggcctgca gctagctagg ccggaaacca gcgtgcatgc aaagatggtc ggcgtattga
     2641 tcgctggaat ggatcccttc tatttgccct ttctgcaagg ggttgaggag gtcgctgaag
     2701 cattaccatc cctggtcgtg atttgcaacg cagaagataa tccggttaaa gccaagctgc
     2761 agatcaacca gttgattctg cgcggtatgg atggcattat tgctgcctcg gttgggaatc
     2821 ttgtgcaaag agagcttgga tttcctcaga caggcggtcc ttttccaatc gtctactgcg
     2881 atcagccaga tacgagcggt catgtcatcc tgttcgacgc tgaaaacgct ggatatcagg
     2941 cggtaaaaca cctgctggac catggtcacc gccgtattgg tatcgtcact gcccccatag
     3001 attggccgaa tatgagcgaa tgctttgctg gctatcgccg agccttaatg gaagctggca
     3061 ttgcgccgcg tcaatctctg gtgtcagtca cgaaaggttt ctcgatttca caggggagcg
     3121 atgcggctcg ctcattgctg gtaggctcca atcctccaac cgccatgttc gccaccgctg
     3181 atatcctggc aattggcgct caacgcgcca tcaaatccga gggattgcgc gtgccagaag
     3241 atgttgctat gatcggttat aacgatatcg agatcgcgtc tatggtggaa ccagcgttga
     3301 ccagcctagc cgccccagcc tatgagatgg gccgcctggc aatggggtta cttgccagac
     3361 ttatcgctgg agagcgcgtt aatcctacta tcacgaggtt ggagaccagt ctagttatcc
     3421 gacgcagttg tggctgctat ccacctgggg aaggggacca tcattaggct cttgcgattc
     3481 ctgaccaccc cattcttgcc aaaaaacgat aacctcattt ggtctcaaca gaacgcgcac
     3541 agcggaaacc aacccgcagg tttggccgga cggagtggga cgagtcacga aagtaggtgg
     3601 tggtttgctc gtaggtagag tcaaacgagc cgccgcgcag tacatgatct gtcacttgtg
     3661 tgggaccggt tgggttgcga tctggtgcat cgtggtaata agacgcttga taatagtctg
     3721 ctacccattc ggccgcgttc ccgcacatat cgagcagccc ataggggctg gcaccgtcca
     3781 gaaagctgcc tactggcagt acgttgccct gaccgttctc tgacgtattg gctttattag
     3841 gatcccactc gttcccccag gggaaatgcc gcccgttgtc gccacgggcg gctttttccc
     3901 actcggcctc tgtgggcagg cgcatgccga tccagcggca gtatgcgtcg gcattttccc
     3961 ataggatacc aaccacgggc agctggctgt atgcctcaag tttagattgg tccgtatcgg
     4021 gcgctgggta gccgatggct gcgagatact ggatataatc gctacgggta acctcagtgc
     4081 gctgaatttc aaacgtatcc agatagacgc gatgctgggg ttggctcgac cttctggcat
     4141 cgtcctcgcc catccagaac cagccagcag gcacctgaac caggtcggat tttggtatgg
     4201 ttggcgtagg ggttggacga ctgcagcctg ccagtcccag cagggcgatt gcgagcaagc
     4261 tggcggatct tccccacata ttcatacgga taactttctg acacaaagga cacgaatgtt
     4321 aaacatacgg ttaaataatc cttttccttt cgtgttcctt tgtgttcttg gtggtgagat
     4381 ttaatgtccc agtggataat gcccgaactc ttctaccgcg tcaaccatcg ccaggatgtt
     4441 ctctggcggg acttcatcca ggatggtgtg caccgcagca accatgtagc cacctcctgg
     4501 tcccaggatg tcaatcaccc gccgcacttc ctggcgcacc tgttcaggcg tgccgaaagg
     4561 cacgatgcgg tacgtgtcaa ccgccccgca taagcataga ttcctgccgt agcgtttctt
     4621 gattgccacc agatcaacca tcttacctgc cgaagtctgg atgggattga ggatgtctac
     4681 accgatctcg atgaaatcat cgatgaggtc aaacacatca ccatccgtgt gaaaaaatac
     4741 tttagccttc gtatgtgctt tgatgaaagc aatata
//
LOCUS       DBNHOH010000654         3728 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1193343, whole genome shotgun sequence.
ACCESSION   DBNHOH010000654 DBNHOH010000000
VERSION     DBNHOH010000654.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1193343"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="AC16V7_11385"
     CDS             <1..380
                     /locus_tag="AC16V7_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015198909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HIR6257766.1"
                     /translation="EAAEAVLVYLRGCQIVADKGFIGADWQAQIYAWTQNQIWTPKRS
                     NQYQQNPKGLDRWLNSVRERIEGVFHELTNTGRDLEGLLAKTLVGLCTRVIAKMASHA
                     LKHVLNFQFGIKVQTFKMAASAA"
     gene            528..854
                     /locus_tag="AC16V7_11390"
     CDS             528..854
                     /locus_tag="AC16V7_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6257767.1"
                     /translation="MMQPEEIEQMKMSPSWAGRIMSAHTLSREMRAHDQYVFNTERFK
                     NMKIPTLLLVGGDSPESLLGSTNALASVLVNCQVKVMPGQQHVAMLTAPEMFADLVAQ
                     FILESV"
     gene            953..1081
                     /locus_tag="AC16V7_11395"
     CDS             953..1081
                     /locus_tag="AC16V7_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257768.1"
                     /translation="MDDFLHGVKYPVILKSDDNQSIAQRRPTWHATDRFALRARGG"
     gene            1122..1703
                     /locus_tag="AC16V7_11400"
     CDS             1122..1703
                     /locus_tag="AC16V7_11400"
                     /inference="COORDINATES: protein motif:HMM:NF025160.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HIR6257769.1"
                     /translation="MSSNSQAISTHPQEAGRRSVAWILWLLPIVFIIHDGEELLVIPS
                     WIASHQQELNQLAKMSEVAAKLMRSLPTTTKQVAIAIGFILFLFIAVTVGASMSSKRG
                     FWVYAYASLLGGLFLHVFTHIAQAMFVGGYAPGVISAVAAVIPGTLYIYKRLFDAKFL
                     TLKSAAFAALIGLALFVPGAMLAHQIGRVLGSG"
     gene            1976..3334
                     /locus_tag="AC16V7_11405"
     CDS             1976..3334
                     /locus_tag="AC16V7_11405"
                     /inference="COORDINATES: protein motif:HMM:NF024240.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6257770.1"
                     /translation="MSDIKEAVGPLLTMRYDVVVAGGGPAGTVAAIAAARLGAKTLLV
                     ERGNCLGGTATAGLIVQFGGGGYAFMTGIMKEFLDELIKRGAAIDLGLPNVNYPFDPD
                     GFKDLAIEKLSQSGADLLLYSPVVGVLLEGTAVRGILAENKSGRCSIEAAVVIDATGD
                     ADVVARSGADVLKLPGGGILGAIIGNIDFAELRAYVARYPDQIFETDLSKPLIRIAGF
                     YDVVEEALARGDLEVDMMRIPRHLVETEGTIEGTRQYLRVDGIFEEKGLAMIGYGAHV
                     DFDATDAFSLTRAEMEARRRQRVLLDFFRKYIPGFRNCYIVQTASSMAVWKSRKIIGD
                     YVLTSDDIRTSKRFYDVVVNAHTNLDHRSLYIKEPLEFDIPYRAMLPRGYDGIVVAGR
                     CISTTATVQIPGFNIPLCMQMGQAAGTAAAMAAAGQISPRLLAFEQLQRRLSIDGVLW
                     RM"
BASE COUNT          881 a          945 c          996 g          906 t
ORIGIN      
        1 gagaagctgc cgaagccgtt ctggtctacc tgagaggctg ccagatcgtc gcagacaaag
       61 gcttcatcgg tgcagattgg caggcgcaaa tctatgcctg gacccagaac cagatttgga
      121 ctcccaaacg ctcgaatcag tatcagcaga acccgaaggg attggatcgt tggttgaaca
      181 gcgttcgaga acggatcgaa ggcgtcttcc acgaattgac caataccggt cgtgacctgg
      241 aaggactgct ggccaaaacc ctggtaggtt tatgtactag agtgattgcg aagatggcta
      301 gccatgctct caagcatgtg ctcaatttcc agttcggtat taaggtgcag actttcaaga
      361 tggccgcttc tgcagcctaa ttcacatcag acgtccttta tgaacctgct gtaaacctgt
      421 ccctccctgc gagtgccggt cccgatcaaa ttacagaaaa aatacaaagg ttattagacg
      481 aaggtaatcg agatgatgct ctaatattgt tctacagcaa agttgctatg atgcagcctg
      541 aagagatcga acaaatgaaa atgtcacctt catgggcagg acgcattatg tcagcacata
      601 cgttgtcaag ggaaatgcga gcgcatgacc aatatgtatt taacactgaa cgatttaaga
      661 atatgaagat tccaacttta ttgcttgttg gaggagatag cccagaatct ctcttaggga
      721 gcacgaatgc attggcatca gtcttagtga actgtcaagt aaaggttatg ccagggcaac
      781 aacatgtcgc tatgttgacc gctccagaaa tgtttgccga tttagtggca caatttatac
      841 ttgagtctgt ctagtgtatg gtctagccgc ccaacaaagc gttctcagaa acagcttatg
      901 cgggggatag ctggaaacca agttcggcag ccatacgctc caggtaaact ttgtggacga
      961 ttttctacat ggagtgaagt atccggtgat actcaaatca gatgacaacc aatccatagc
     1021 acaacgccgc ccaacatggc atgcaaccga ccgcttcgcg ctacgcgctc gcggcggctg
     1081 atgcgtggcg ttaggtggct ctgcttaaat tgagtaagtg gatgtcgagt aatagtcaag
     1141 ccatttctac acatccccaa gaggctggta ggcgaagtgt cgcgtggata ctttggctgc
     1201 tgccaattgt attcatcatt cacgatggcg aagaattgct cgtcatacca agttggattg
     1261 ctagtcatca gcaggaactt aatcagcttg ccaagatgag cgaagtagca gcaaagttga
     1321 tgcgttcact gcccacaact acaaaacagg ttgctattgc gatcggattc atcctgtttc
     1381 tgtttattgc ggtgacagtt ggcgcgtcca tgtcaagcaa gcgcggtttc tgggtatacg
     1441 cttatgccag tttgcttggt ggtttattcc tgcatgtgtt cacgcacatc gcacaggcaa
     1501 tgttcgtcgg cggatatgcg cccggggtaa tcagtgcggt agcggcagtc attcccggca
     1561 cgctctatat ctacaaacga ctctttgatg caaagttttt gacattgaag tcagcggctt
     1621 tcgcagcgct tatcgggctt gctttgttcg ttccgggagc gatgttggcg catcaaattg
     1681 ggcgagtgct cggtagtggt tagtcgccac ctaacacagc gttctaagga agtgttgtaa
     1741 aacacttcct ttttggaaac atgatggccc tctatcttga ttaggtgcac ttggctggtg
     1801 ggattcgccg cgatttacaa gcctttttct cgcttcgggt ttatactact ctcaagcgtt
     1861 atctcgtccc gcccacgagc cggtaacgcg agccgttagg ctgtagacgt gctctcagca
     1921 atagatcgta ccgtgaccca gtaagcgcaa gacatcggag aggagccaag tacccatgag
     1981 cgacatcaag gaagctgtcg ggccgctgct gacaatgaga tatgacgtcg tggttgccgg
     2041 aggcggccca gccggcacgg tcgcggctat cgcggcagcc cggctgggtg ccaagacgct
     2101 gttggtcgaa cgcggcaatt gtcttggggg cacagccacc gcaggtctca ttgtccagtt
     2161 cggagggggt ggatatgcct tcatgaccgg gatcatgaag gagtttcttg acgagctaat
     2221 caagcgcggc gcggcgatcg atcttggcct gcctaacgtg aattacccgt tcgaccctga
     2281 tggtttcaag gatctcgcca tagagaagct cagccaatct ggcgcagacc tgctcctgta
     2341 ctctccagtt gtcggcgtgc tccttgaggg gacagcagtt cgtggcatac tcgcagagaa
     2401 caaaagcgga cgatgctcaa tcgaagccgc agtcgtcatt gacgccactg gcgacgctga
     2461 cgtagtcgcc cgctcgggtg ccgatgtcct gaagctgccc ggcgggggga tactcggcgc
     2521 tatcattggg aacatagact tcgcagaatt gcgggcatac gtggctcgct acccggatca
     2581 aatcttcgag accgatctgt cgaaacctct gatcagaatc gccggcttct atgatgtcgt
     2641 cgaggaggcg ctagcacggg gtgaccttga ggtagacatg atgcggattc cgcgacacct
     2701 tgtcgagacg gaaggcacaa tcgagggaac gcgccagtac ctgcgtgtag acgggatctt
     2761 cgaagagaag ggactggcta tgatcgggta tggcgcccac gtggatttcg acgcgacgga
     2821 cgcattctcc ttgacaaggg ccgaaatgga agctcgtcgg agacaacgtg tgttgctcga
     2881 cttcttccgt aagtacatac ccggattccg caactgctat atcgtccaga ctgcatcgag
     2941 catggcagtc tggaagagcc gaaagatcat cggcgattat gtgttgacaa gcgatgatat
     3001 tcggacttca aagagattct atgatgtcgt ggtgaacgct cacacaaatc tcgaccatcg
     3061 ctcgctctac atcaaggaac cgcttgagtt cgacattccc tatcgtgcaa tgttgccacg
     3121 tggctacgat gggattgtcg tggcaggaag gtgcatttcc actaccgcca ccgttcagat
     3181 acccggcttc aacatccctc tatgcatgca gatggggcag gcggcgggca cggcagccgc
     3241 catggccgcc gcaggccaga tatcgcctcg gctcctagcc ttcgagcagc tgcagaggag
     3301 gttgagcata gatggggtcc tatggaggat gtaatgagtc ctcctcttcg catcgtggcc
     3361 gtctggatac agagaatgat gccttccccc taacaggcgg gtgaatggaa taatcttctc
     3421 gctttataat aggaaacgga ctgaatgaca tgattgctcc acaagatact ttactgtccg
     3481 tccagcatca cacttggaaa cagacattac tctttcacct tgttccaggt atctgcatca
     3541 catgtgcttt tgtcttacta acgcgactta ctataccttt cggctggccg cctagccttg
     3601 cactcctact cacctggtta atcattggaa ttccgttact catcggcata ctgttcttcc
     3661 aagcccaccg actcaacggc actctatcgc tcagcaggat ccttttgtct cgtcaacctc
     3721 tgccctgg
//
LOCUS       DBNHOH010000655         5171 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1577523, whole genome shotgun sequence.
ACCESSION   DBNHOH010000655 DBNHOH010000000
VERSION     DBNHOH010000655.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5171)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1577523"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..2078
                     /gene="uvrB"
                     /locus_tag="AC16V7_11410"
     CDS             36..2078
                     /gene="uvrB"
                     /locus_tag="AC16V7_11410"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HIR6257771.1"
                     /translation="MSDAFHLVSEYAPTGDQPAAIAGLVEGVRRGDPHQTLLGVTGSG
                     KSFTVACVVERIGRPTLVMAPNKTLAAQLYSEFKQLFPENAVEYFVSYYDYYQPEAYV
                     PRHDLYIEKDSDINEEIERLRLAATTALMSRKDVIIVASVSCIYGIGNPDAYGRVVIN
                     LESGTVYRRNALLRQLIEGQYERNDLELSPGKFRVRGDTLEIIPAYQDQFGYRITFFG
                     DEVEKVVEFDAITGELRRQLSTVHIYPAKHFITDQDKLNQAIEDIQAEMVERLALFKS
                     QDKHLEAQRLEQRTYYDLEMLREVGYCSGIENYSRHLDQRPQGSPPWTLMDYLPSEYL
                     LVIDESHMTIPQVRGMFNGDRSRKETLVEYGFRLPSALDNRPLMFDEFERRMGFTIYT
                     SATPGPYEMKRNDQVVEQIIRPTGLVDPEVEVRPIEGQIDDLLAEIRQRVEVGQRTLV
                     TTLTKRMAEKLADYLMELGVKVHYLHSEIDTLERVGILRDLRLGVFDVVVGINLLREG
                     LDLPEVSLVAILDADKEGFLRSETALIQTIGRAARHVDGRVIMYADKITDSMRRAIDE
                     TNRRRAKQVAYNQEHGIQPVSIYKAVRDLTDQLSVRAVAESKPEYRVRGGSMERSEMQ
                     RVINELEKQMKEAAQNLEFEKAAVLRDQVFELRGLLAEDSNLPPWKKVRLLAGEED"
     gene            2251..2772
                     /locus_tag="AC16V7_11415"
     CDS             2251..2772
                     /locus_tag="AC16V7_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257772.1"
                     /translation="MKKFLVISAVAILAILVLGVSGFAYAQAQTPPPPDYPNYGPGMM
                     GRWGGRGFGQGMMGGWGQGGYGPMHEYMEDAFAEALNLTHEELEAKLADGETMWSIAQ
                     AQGLSDEEIADLMVNARTKALEQAVADGVLTKEQADWMTQRMQEMQSAGFGPGYCHGF
                     SGQGRGSGWRNTP"
     gene            2862..3566
                     /locus_tag="AC16V7_11420"
     CDS             2862..3566
                     /locus_tag="AC16V7_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6257773.1"
                     /translation="MATILVIEDEPELVKVLRSYLEQAGFSVLTAARGDTGLSTWEHK
                     RPDLVLLDLNLPGMDGLDVAREIRRKSETPIIMLTARVEEIDQLIGLELGADDYIPKP
                     FSPRLVVARVRALLRRAASTPTASQVLRIADLEIDLDAYTVARSEQLLELTPTEFNLL
                     AAMAAQPGRVFTRLQLLEAVQGVAYEGYERTVDAHIKNLRAKLEPDPKQPRYIETVFG
                     IGYRFRKETRDLPSEA"
     gene            4133..5017
                     /locus_tag="AC16V7_11425"
     CDS             4133..5017
                     /locus_tag="AC16V7_11425"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6257774.1"
                     /translation="MVAGIASLLLALVLAYRLLRPIRALTRAAEQMGGGDLSQRVPVH
                     GDDELALLGTTFNRMAASLQRAEESRQAMTADIAHELRTPLAVQRASLEALQDGIYPL
                     NPENLAPILEQNILLNRLVDDLRTLALADAGQLQLEIIPTDFDGLVEHVVELFTPQAA
                     SQSIDLHIDNSPAHLSPIPLDPLRVEQILGNLLSNALRHAPPHSQVDIAISHTPTAVQ
                     LSVRDSGPGISPEALPRVFERFYRADKSRSRVEGGTGLGLAIARQIAQAHGGNLTADN
                     HPLGGAIFTLTLPSAQNP"
BASE COUNT         1147 a         1510 c         1449 g         1065 t
ORIGIN      
        1 gaggacacgc ccgacgggcc gcgctggagg cgccggtgag cgacgccttc cacctggtct
       61 ccgaatacgc cccgacggga gaccagcccg ccgccatcgc cggcctggtg gaaggcgtcc
      121 ggcgaggcga cccgcaccag accctgctcg gcgtgaccgg gagcgggaag tccttcaccg
      181 tcgcctgcgt ggtcgagcgg atcgggcgtc cgacgctcgt gatggcgccg aacaagacgc
      241 tcgccgccca gctctattcg gagttcaagc agctgttccc cgagaacgcc gtcgagtact
      301 tcgtctcgta ttacgactac taccaacccg aggcctacgt cccgcgccac gacttgtaca
      361 tcgagaaaga tagtgacatc aacgaggaga ttgagcgcct gcgcctcgcc gcaactaccg
      421 ccctgatgtc acgcaaggac gtgatcatcg tcgcctccgt ctcgtgcatc tatggcatcg
      481 gcaatccaga cgcatatggg agggtggtaa tcaacctaga gagcggcacc gtctatcgcc
      541 gcaacgccct gcttcgccaa ctcatcgaag gacagtacga acgcaacgac ctagagctaa
      601 gcccaggtaa attccgcgtg cgcggggata cactggaaat catcccggcg taccaggacc
      661 aattcggtta tcgcatcaca ttttttggcg acgaggtaga gaaggtggtg gaatttgacg
      721 ccatcacggg cgagttgcgc cgccagctaa gcaccgtcca catttatcca gccaagcact
      781 ttatcactga ccaggataag ctcaatcagg ctattgaaga tattcaggct gagatggtgg
      841 agcgccttgc cttgtttaag tcgcaggaca aacacctgga ggcacagcgc ttggagcaac
      901 gcacgtacta cgatctagag atgctgcgcg aggtgggtta ctgctcggga atcgagaact
      961 actcccgcca cctagatcag cgcccgcaag gctcacctcc ctggacgctg atggactacc
     1021 tgcccagcga gtacctgctg gttatcgacg aatcacacat gaccatcccc caagtgcgcg
     1081 gcatgtttaa cggcgatcgc tcgcgcaaag agacattggt ggaatatggc ttccgattgc
     1141 cgtctgcgct ggataaccgc ccacttatgt ttgacgaatt cgagcggcgc atgggcttca
     1201 ccatttatac atctgctacg cccggcccat acgagatgaa acgcaacgac caagtcgtgg
     1261 aacagatcat acgccccacc gggttggttg accccgaagt ggaagtgcgc cccatcgaag
     1321 gccaaatcga cgacctgcta gccgaaatcc gccagcgggt ggaagttggt caacgcactc
     1381 tggtgaccac cctgaccaag cgcatggcag agaaacttgc agattacctg atggagctgg
     1441 gtgtcaaggt tcattatctg cattccgaaa ttgataccct ggagcgggtg ggtatcctgc
     1501 gtgatttgcg tctgggggta tttgacgtgg tggtcgggat aaacctgcta cgcgagggtc
     1561 ttgacctgcc cgaggtctct ctggtggcaa tcctagacgc agacaaagag ggcttcctgc
     1621 gctccgagac tgccctgatc caaaccattg gtcgcgccgc ccgccacgta gatgggaggg
     1681 tgatcatgta tgcagataag atcaccgact caatgcgccg cgccatcgat gagacaaatc
     1741 gccgccgcgc caagcaggta gcctataacc aggagcatgg catccagcca gttagcatct
     1801 ataaggccgt gagagacctg accgaccagc tatccgtgcg tgccgtcgcg gagagcaagc
     1861 ccgaataccg cgtacggggt gggagcatgg aacgctcgga gatgcagcgt gtcatcaatg
     1921 agttggagaa gcaaatgaaa gaagcggctc aaaacctgga gtttgagaag gccgccgtgc
     1981 tgcgcgacca ggtatttgag ctgcgcggcc tgctagccga ggattcaaac ctgccgccct
     2041 ggaagaaagt tcgcctgctg gcaggcgaag aagattgacc accgcctaat atacaattgt
     2101 tttagcgggg aaaaacatct tgattactgt gcaaaccttc acgaagtgta cagaagctct
     2161 tcacaacttc ttcacaaccc tgcgctaaac taaggacaga ttggtaaacc aaacaaccaa
     2221 cacatatcac ttcattaagg agtatttgag atgaaaaagt tcctagtaat tagcgcagtt
     2281 gccattctgg ccattctggt tttgggagtt tcaggctttg cctatgctca agcacagacc
     2341 cctcctccgc ctgattaccc taattatggc cccggcatga tgggccgctg gggtggtcgc
     2401 gggtttggcc agggtatgat gggtggctgg ggtcagggcg gctatggtcc gatgcacgaa
     2461 tacatggaag acgccttcgc agaggcattg aatctgactc acgaagagct agaggccaag
     2521 ctagcagatg gtgaaaccat gtggagcatc gcacaggctc aaggcttatc cgatgaggag
     2581 attgccgacc tgatggtaaa tgcccgcaca aaagccctgg aacaggccgt tgctgacggt
     2641 gtactcacca aggagcaggc tgactggatg acccagcgca tgcaggagat gcaatcggcc
     2701 ggttttggcc caggctactg tcacggtttc tccggtcagg gtcgcggttc gggctggcga
     2761 aacacaccat aacatatccg tagagaaggg ttgcgggtgt ttcagcaccc acaacccttt
     2821 tttttcggct tccccttgct tgttgtataa tgtgcgcggt aatggcaacg attttggtta
     2881 ttgaagacga acccgagttg gtcaaggtgc tgcgctccta cttggagcaa gccggtttca
     2941 gtgttttaac cgcggcgcgc ggcgatacgg ggctgtccac ttgggagcac aagcgcccag
     3001 acctagtgtt gctcgatctg aatctgcccg gcatggatgg attggatgta gcccgtgaga
     3061 tccgccgcaa gagcgaaacg cccatcatta tgcttaccgc ccgcgtcgaa gagatcgacc
     3121 agttaattgg cctggagcta ggtgctgatg actacatccc caagcccttc tcgccgcgcc
     3181 ttgtagtggc gcgcgtgcgc gctctacttc gccgagccgc ttccactcca acagcttctc
     3241 aggtattgcg cattgctgat ctggagatcg atcttgacgc ctataccgtc gcccgttctg
     3301 aacaactgct ggagttgaca cccaccgaat ttaacctcct ggcagcgatg gcggctcaac
     3361 ccgggcgtgt ttttacccgc cttcaactgc ttgaagcagt ccagggtgta gcttacgaag
     3421 gctacgaacg taccgtcgat gctcatatca agaacctgcg cgccaagctg gaacccgacc
     3481 caaaacaacc ccgttacatc gagactgtat tcggtattgg ataccggttt cgtaaagaga
     3541 ccagggactt gccgtccgaa gcctaaataa ataagcttga caccctgata ccacaaccaa
     3601 gacctctcat cgccaagcta tgcgtttacg tttattcctt tcctttgcca tcatcgtgct
     3661 ggtctccatt gccagcgtag tagtcattgc ccggcaaggc gccgccaccg aggtgcgcgc
     3721 gtttatgttt cgtggcggca tgtcagggac agagagtttg gttagctcac tggaagcgta
     3781 ctacgccgcc cacgattcct ggcaaggggt cgagcagctt cttctcgaat cggggccagg
     3841 gcgcgggcac ggcatgcctg gcatgggagg caacggtctg gggccgggtg gcatggcggg
     3901 gatgatggcc cagcgcctgc ggctcgccga tgcgcagggc gtgctagttt tcgataccag
     3961 ccagaacggt gtgggaggta cgctcagctc agacgaactg caaagtgcaa tctccctgcg
     4021 ggcaggcgga aaagttgtcg gtttcctgct ccccgaaggg ggcatggcct tctcgcccgg
     4081 cgacgagacc cgcctggtca gccgcatcac acgcgctgcc ctggttgctg ggatggtcgc
     4141 aggtatagcg tcgctgctct tggcgctggt tctcgcctat cgtttgctcc gacccatccg
     4201 cgcactgact cgcgccgccg aacagatggg cgggggcgac ctctctcagc gagtgccagt
     4261 tcacggagac gacgagctgg ccttgctagg cacgacgttt aaccgcatgg ccgcatcttt
     4321 gcagcgcgct gaggagagcc gccaggcgat gaccgccgat atcgcccatg agctgcgcac
     4381 tcccctagct gttcaacgcg ccagcctgga agcccttcaa gacggcattt acccgctcaa
     4441 tccagagaac ctggcgccca tactggaaca gaatattctg ctcaatcgac tggtggacga
     4501 cctgcgaacc ctggccttgg ccgatgccgg ccagttacag ttagaaatta ttcccacaga
     4561 ttttgatggg ctggtcgagc acgtcgttga acttttcacc cctcaggctg cttcccaatc
     4621 aattgacctg catattgata attctcctgc gcatttatct cccatcccgc tggatccact
     4681 gcgggtcgag caaatcctgg gcaacctgct atctaatgcc ctgcgccacg cgcccccaca
     4741 tagtcaggta gatatcgcta tctcacatac gcctaccgca gttcagctga gtgtgcgcga
     4801 cagcggccca ggtatctccc cagaagcctt gccgcgcgtg ttcgaacgct tttaccgcgc
     4861 cgacaaatcc cgaagccgcg tggagggcgg caccggcttg ggcctggcaa ttgcccggca
     4921 gattgcgcaa gcgcatggag gcaatttgac tgccgacaac catcccttgg gaggggcgat
     4981 cttcacatta accctgccat cggcacaaaa cccataaagc gtgttcgtat tacaatattt
     5041 ctcaatcatt tcattgaaaa gatgatcacc aacaatgtca cccgactgct cgatcagcga
     5101 aagataaatt acaacgcctt cgagctgcca cctgaaaaac tgggcgccat agaagcagcc
     5161 caattcctgg g
//
LOCUS       DBNHOH010000656         1597 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1385682, whole genome shotgun sequence.
ACCESSION   DBNHOH010000656 DBNHOH010000000
VERSION     DBNHOH010000656.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1385682"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="AC16V7_11430"
     CDS             complement(<1..817)
                     /locus_tag="AC16V7_11430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR6257775.1"
                     /translation="MSFNRLSIEQVLYGLAILLALTVRLLNLGAIPLADSEALLALQA
                     FDVSQGEKVAIDPQPGFAALTGLTFALLGSSEFTARLWPALAGGLLVIVPYFLRRQLG
                     RGAAIILAFGLAFDPGLVAVSRQADGPMLSVGFSLLVLGLAIAGYPILAGVAAGLALL
                     SGPALLPGLLGLALAWGASLLFSDTEEGDQLLPAAWDEKALRNGVLAMLGTLVLVGTL
                     FLRAPQGLGGWIAGLPSYLSGWAQPSGVPALRLVIALVVYQPLVVIFGLIGAIQ"
     gene            complement(954..>1597)
                     /locus_tag="AC16V7_11435"
     CDS             complement(954..>1597)
                     /locus_tag="AC16V7_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257776.1"
                     /translation="NTIFKFYYQAWLLWAVAAAYASSILLLIPRRPWNLIYRGVLILI
                     MMAGMTYTSLGLWTKTNAFKPAMGLTLDGTAYLLNQSPNEMSAIAWLSAAPAGVVAEA
                     VGGSYSIYGRVSTLSGQPTVLGWDFHETQWRGSSLLLGTRQNDIQRLYCTRDWNEAQQ
                     IINLYNIRYIFLGNLERSDFTPANCPGGLNDVKFIRNLAVAFQQGEVTVYMVP"
BASE COUNT          433 a          453 c          408 g          303 t
ORIGIN      
        1 cttgaattgc cccaatcaat ccgaagatca ccaccagtgg ttggtagaca accagcgcaa
       61 tcacgaggcg aagggcaggt acgcccgacg gctgcgccca cccagataaa tacgacggca
      121 atccagctat ccagccgccc aaaccttgcg gggcgcggag aaataaggta ccaaccagca
      181 caagcgtccc aagcatcgcg agcacaccat tgcgcagggc tttttcatcc cacgcagcag
      241 gaagcaattg atccccctct tctgtgtctg agaatagcag gctcgctccc caggcaagag
      301 ccaggccaag caggccaggg agcagcgccg gtccggagag caaagccaac ccggcagcca
      361 cgccagccag gatcggatat ccggcaatgg caagacccaa cactagcaaa ctaaagccta
      421 cagacagcat cggaccatcc gcttgccgcg agactgccac caaaccaggg tcgaaagcca
      481 ggccgaaggc taggatgatt gctgctcctc gccctaactg gcgccgcagg aaatatggca
      541 caatcaccag tagcccacct gctagggcag gccatagtcg ggcagtgaac tcgctgctcc
      601 ctaataacgc gaaggttagc ccagtgaggg cagcgaaccc aggctgagga tcgatagcca
      661 ccttctcgcc ctgagataca tcaaaagcct ggagcgctaa tagcgcctca ctgtcagcaa
      721 gcggtatcgc tcccaagttt aataatcgca ctgtcaatgc gagcagaatt gccagcccgt
      781 agagcacctg ttcgatggat agacggttga agctcataat caatcagcca ttgaattaga
      841 tagtcaaaaa tatgtcatac aagcgattta aattatactg aaaaacagac ggcggggcat
      901 ctggtaccct tgatacgctt gggggaagtg gttccagcca tgaaataaat ctcctaaggc
      961 accatgtaga ccgtgacctc accctgctgg aaagcgacag ccaggttgcg aataaatttg
     1021 acgtcgttca acccaccggg gcaattggct ggtgtgaaat cgctgcgctc caagttcccc
     1081 aagaagatat atcggatatt atacaggttg attatctgct gcgcctcgtt ccaatcacgg
     1141 gtgcaataca ggcgctggat atcgttttgg cgtgttccca gcagtaaact actgccgcgc
     1201 cattgcgttt cgtggaaatc ccaacctaac accgttggct gaccgctgag ggtcgataca
     1261 cgaccataga tgctataact gccccccaca gcttccgcaa ctactcctgc aggtgcagcg
     1321 ctcagccagg cgattgcgct catctcgttg ggcgactggt ttaataaata agctgtgcca
     1381 tccagggtaa gacccatcgc aggtttgaaa gcattcgtct tcgtccacaa gccaagggaa
     1441 gtgtacgtca tcccagccat catgatcagg attaaaacgc cgcggtatat aaggttccaa
     1501 ggacgtctag ggatgagcaa gagtatcgat gaggcgtaag cagccgcaac tgcccagagc
     1561 agccaggcct ggtaataaaa tttaaatatg gtgttga
//
LOCUS       DBNHOH010000657         1242 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_359713, whole genome shotgun sequence.
ACCESSION   DBNHOH010000657 DBNHOH010000000
VERSION     DBNHOH010000657.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_359713"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1180)
                     /locus_tag="AC16V7_11440"
     CDS             complement(128..1180)
                     /locus_tag="AC16V7_11440"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase FemX"
                     /protein_id="HIR6257777.1"
                     /translation="MNNNHHWDQQGFHLVHSLEDDAWKAFVDNHPQGNIFHTPEIFEV
                     FKHTQGYRPSLWAVRGSDGQILALMLPVEITLSNVFRRLTSRNVVYGSILYEQSEKGK
                     EALEMLLKAYIQKTGGKPLFTELRNISDLSPIQSLLQKCGFSYEDHLNYLINLDHPVD
                     AVFQNIGQRTRKNIRKAINRGVVQIEEVQDLEQLEKCYQLIWKTYRAWGIPSPHNTLF
                     TAAHNILRPKEMVRFTLARVDDALVAVSVDLLYKHVIYGWYGGMERAYSAYSANELLT
                     WHLLKWGAEQGYHVYDFGGAGKPDEEYGVRDFKAKFGGELVCYGRNTYVHAPITLSIS
                     KIGYQIYQGIVESRSP"
BASE COUNT          311 a          303 c          292 g          336 t
ORIGIN      
        1 gatggcaaat ttatcttgag taaagcgtct cccaaatatc gatccggtgg taacaaccaa
       61 ggggaatgct ctgcaggcaa acaattgatt tatttcccag atctattgtg gaagaaagaa
      121 gcagtgatca aggggagcgg gattcgacga taccctgata aatttgatac cctattttgc
      181 tgatcgatag cgtaatgggg gcatgcacat aggtattgcg accataacaa accagttccc
      241 cgccaaactt ggccttgaaa tctcttaccc cgtattcctc atcgggcttg ccagcgccgc
      301 cgaagtcgta cacatggtag ccttgttcgg ctccccattt caaaagatgc catgtcagca
      361 actcgttcgc tgaatacgcc gagtacgccc gctccatgcc gccataccag ccatagatca
      421 catgtttata cagcaaatcc accgatacag ctaccaaggc gtcatccacg cgtgccaggg
      481 taaagcgcac catttccttt ggtctgagta tattatgggc agctgtaaat agcgtgttgt
      541 gtggggaagg tatgccccag gcgcgatagg tcttccaaat gagctgatag cacttttcca
      601 attgctccag gtcttgcacc tcttcgattt gcaccactcc acggttgatc gctttgcgta
      661 tattcttgcg cgtccgctga ccaatgtttt gaaagacggc atccactgga tgatccaggt
      721 tgatcaggta atttaggtga tcctcataag aaaagccgca tttttgcaag aggctttgga
      781 ttgggctcaa atcggagata ttgcgcagct cggtgaatag cggtttccca cctgtctttt
      841 ggatgtatgc tttcaggagc atttccaatg cttctttgcc tttttcgctc tgctcgtaca
      901 agatgctccc gtagactaca tttcgactgg tgagtcggcg aaacacattg cttaaagtga
      961 tctcgacggg tagcatcagt gccaggattt gtccgtcgct acccctaaca gcccatagcg
     1021 atggacgata accctgagtg tgtttaaaca cctcgaaaat ttctggtgtg tggaagatat
     1081 tcccttgggg atgattatcg acaaaggctt tccaagcatc gtcttccagg ctgtgtacta
     1141 aatgaaatcc ctgttgatcc caatgatgat tattattcat gtctggtatg tccagaagga
     1201 agataagaag agtaactcag gttacgctgc gccagctacc aa
//
LOCUS       DBNHOH010000658         2196 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1128645, whole genome shotgun sequence.
ACCESSION   DBNHOH010000658 DBNHOH010000000
VERSION     DBNHOH010000658.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1128645"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..531)
                     /gene="sufU"
                     /locus_tag="AC16V7_11445"
     CDS             complement(136..531)
                     /gene="sufU"
                     /locus_tag="AC16V7_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HIR6257778.1"
                     /translation="MDDFYRELIIDRYKNPQFRGLLDPHDISFEDENPLCGDHIRIDV
                     RVDGNGTVTEAAFDGHGCAISQASADLLLESIVGKPLEDIKEMNREDILDLLGIELGP
                     VRLKCALLSLKVLKAVVYGLGEASDDLVE"
     gene            complement(532..1779)
                     /locus_tag="AC16V7_11450"
     CDS             complement(532..1779)
                     /locus_tag="AC16V7_11450"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012187968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - obsolete cysteine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HIR6257779.1"
                     /translation="MTIHTDNKLNVQRIRQDFPILTREVRPGVPLVYLDSTATSQKPQ
                     VVIEAMDNFYRRSNANIHRGIHTLAEESTAAYEAARQQVADFIHAPSARQVVFTRNTT
                     ESINLVAQSWGRANMNPGDVIILTEMEHHSNLVPWQILAAEKNLRLEFIPVTPDGLLD
                     LESYRELLRLEPKLISFTHMSNVLGTINPAEEIVQLAHQAGAVTLIDAAQSVPHFSVD
                     VQELDADFLAFSAHKMCGPSGIGALYGKKALLEAMPPFLGGGDMIKRVELRSFVPNSL
                     PHKFEAGTPAIAEAVGFAAAVDYLSKVGMDAIAAHEHEIVEYALERLEEVPGVSMFGP
                     AAQFRGGVAAFTLAGVHAHDVAQVLDTDGIAVRAGHHCAMPLHNKFNLPATARASFYL
                     YNTRQEVDMLAESLYKVKTIFRV"
BASE COUNT          576 a          592 c          474 g          554 t
ORIGIN      
        1 atcatcctca ctggcaactg caacaaaatc acactcttcc ggcttcaaat tcttatagtt
       61 gtacatcgaa gtcatatgct ttttgggaaa ttttattatt tatattggct atgcgaaaat
      121 aatcagagaa agaaatcatt cgaccaaatc gtcactggct tcgcccaatc catataccac
      181 ggccttcagc acctttaatg ataataacgc gcatttcaat cgtaccggcc ccaactctat
      241 ccccagaaga tccaaaatat cttccctatt catttctttg atatcttcga gcggtttacc
      301 aacgatcgat tcaagcaaca gatctgcaga tgcttgagat atggcgcacc catgcccatc
      361 aaaagcggct tccgtcactg ttccattccc atccacgcgc acatcgatgc gaatgtgatc
      421 gccacacaga gggttttcat cctcaaatga tatgtcgtgt gggtctagca agccgcgaaa
      481 ttgggggttc ttatagcgat caatgatgag ttcgcggtaa aaatcgtcca tttacaccct
      541 gaaaatcgtt tttactttat ataaggactc agccaacata tccacttctt ggcgggtgtt
      601 gtagaggtag aagctggcac gcgcagtcgc tggcaaattg aatttattat gcaatggcat
      661 agcacagtga tgacctgcgc gcacagcaat gccatccgta tccaaaacct gcgcgacatc
      721 gtgagcatgt actccagcca gtgtaaatgc tgccacacct ccccggaatt gagcagcagg
      781 accgaacatg cttacacctg ggacctcttc caatcgttcc agggcatact caacaatttc
      841 atgctcatgc gcagcgatgg catccatacc cactttcgag agataatcca cggccgctgc
      901 aaagcccaca gcctcggcga tagcaggtgt gcctgcctca aatttatgcg gcaacgagtt
      961 ggggacaaac gagcgcaact cgacgcgctt tatcatatcg ccacctccta gaaaaggtgg
     1021 catagcctct aaaagcgctt tcttgccata aagcgcacca attccgcttg gcccgcacat
     1081 cttatgcgct gagaaggcta agaaatctgc atcaagctcc tgtacatcca ctgaaaaatg
     1141 aggcaccgat tgcgccgcat cgatcaaggt cactgcaccg gcttgatggg caagttgaac
     1201 gatctcttca gccggattga tggtccctaa aacattagac atgtgtgtaa aagagatcaa
     1261 cttcggctcc agcctcagta actcacggta ggattccaga tcgagtaatc cgtctggggt
     1321 taccgggata aactccaatc gcagattttt ttcagcggcc agaatttgcc agggtaccag
     1381 gttggagtgg tgctccatct cggtcaagat aatcacatcg cctgggttca tgttggcacg
     1441 cccccagctc tgagcaacta aattgatcga ttcggttgta ttacgcgtaa agacaacctg
     1501 ccgagcagat ggcgcatgga taaaatcggc tacttgctgc cgggcagcct cataggctgc
     1561 cgtagattcc tctgcaagtg tatgaatacc tcgatggata ttagcatttg aacgccgata
     1621 aaaattatcc atggcttcga tcaccacctg cggtttttgg ctggtggctg tcgaatccag
     1681 gtataccagc gggacaccag gacggacctc cctggtcaag attgggaaat cctggcgaat
     1741 tctctgcaca ttcagcttat tgtcggtatg gatggtcatg atcgtgcgat ccaaaagata
     1801 agatagcgct gaatacctat ctgacccgca cgcgcggacg cctcttgcgc cgcgcaagtc
     1861 ggacattact agaatttttc gggcaccata gaacatgatc cggtaccatc cacccatcgg
     1921 tcaggtaaac attttcgcga aattgaacgg ctatcaactt cgtgtccact tggacgacag
     1981 taccaaccag ccaatcacgg acccgagtac catttttgtc gtgatcacac agcacctcaa
     2041 cctgatcgcc gacattatat ttttctactc cactaggagt tgttttgaac tttaactctg
     2101 ccaactccgc ctcctgattt aatatttcaa acaccagagg caagctaatg attttatgct
     2161 cgccatgttg ttgccattac ccctccactc gcctgt
//
LOCUS       DBNHOH010000659         3150 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_808770, whole genome shotgun sequence.
ACCESSION   DBNHOH010000659 DBNHOH010000000
VERSION     DBNHOH010000659.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_808770"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="AC16V7_11455"
     CDS             complement(<1..608)
                     /locus_tag="AC16V7_11455"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6257780.1"
                     /translation="MKPEGSKPMGDVGDQTIIQATNIYKTYYTGTVAVPALRGVNLCV
                     RRGEMVAVMGPSGCGKTTLLNCLSGLDYVDSGEIIIEGTSLAKMSDRERTDYRARRMG
                     FVFQFYNLLPVLSAVENVELPLLVAGVRPSEARERALEALAQVGLADRADHRPAAISG
                     GERQRTTIARALANRPAIVWADEPTGDLDSETATDIMDLLLRL"
     gene            complement(751..1926)
                     /locus_tag="AC16V7_11460"
     CDS             complement(751..1926)
                     /locus_tag="AC16V7_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006515372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HIR6257781.1"
                     /translation="MAKPESNLSLSRRAFLRITTALGGAAALISFLEGCSKAEIAQET
                     LISPTDTLEISTLTQEVLPTTSPTQEEIQPTEATAVPSATSTESVENGVARLAFVKTT
                     DRAEGVRRAIDLLGINPVAGKHVFLKPNFNSSDPAPGSTHPEVLVALVKVLKEMGATE
                     ITVGDRSGMGDTRRVMDRIGVFGLADELGFKTIVFDELQAEDWVMIQPPDSHWENGFP
                     FARPCLEAEALVQTCCLKTHRFGGHFTMSLKNSVGMVAKKLPRDDHDFMQELHFSSNQ
                     RRMIAEINAAYAPALIVMDGIEAFISGGPDEGERASPEVVLAGTDRIAIDAVGIALLR
                     YFGCKTEVARGKIFQQEQIARAVDLGLGVDHPEKIEFLTADTDSAAYSEKIKEVLLA"
     gene            complement(2066..2410)
                     /locus_tag="AC16V7_11465"
     CDS             complement(2066..2410)
                     /locus_tag="AC16V7_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HIR6257782.1"
                     /translation="MIFDRRCQEPKYLFHGSYTPEGLKGLLAEGGSSRIEAAKQALKS
                     VGGTLEPFYFAFGDYDFYIIVDMPDNVSTTAISFAGNVSGTFSIKTIVLLTPEEVDEA
                     VKKSVDFRPPGH"
     gene            complement(2683..>3150)
                     /locus_tag="AC16V7_11470"
     CDS             complement(2683..>3150)
                     /locus_tag="AC16V7_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HIR6257783.1"
                     /translation="VSVVPDIPREQFKEILENAVACTQTADFANPLCGSGRFAQVSGF
                     SFTWMASGTAMVIDGDGNVVTDGTRVLDVELDDGTVIVVGGVVVPGPGVTIAIVDFSA
                     KGGDQYPFRGAPLTTIGVTYQQALSNYIQEGLGGLISAADYPEGGEGRITELP"
BASE COUNT          742 a          910 c          741 g          757 t
ORIGIN      
        1 agacgcaaca gcaagtccat gatgtctgtg gcggtttcgg aatctaaatc gccggtgggt
       61 tcatccgccc atacaattgc tggacggtta gccagagctc gggcgatggt tgttcgttgg
      121 cgctcgcctc cggagatcgc cgcggggcgg tgatccgcac ggtctgcaag gccaacctga
      181 gccaatgctt caagcgctcg ctcgcgagct tcgcttgggc ggacgcccgc caccaatagg
      241 ggtagctcga cgttttctac cgcgctcaag acgggcagca ggttgtagaa ttggaagaca
      301 aaacccatgc gccgggcgcg atagtcagta cgctcccggt cggacatctt cgccaatgaa
      361 gtgccctcga taatgatctc cccgctgtcg acgtaatcca gcccagacaa gcaattgagc
      421 agagttgtct taccacaccc agagggtccc atcacggcca ccatctcacc gcgcctgaca
      481 cacaggttga caccacgcaa cgcaggtaca gcaacggttc cggtgtaata agttttatag
      541 atatttgtcg cctgtataat cgtttgatcg ccaacgtcgc ccatgggttt gcttccttcg
      601 ggcttcattc ccgtcccatc cacttctctt tcaaatttac catcaactta aatgatggtt
      661 gagggtcacc ctgtgtcaca tcttgattcc ctcccagttt caatcaacat cctggtgtgc
      721 ataactagca actgtatgtt tacacgatta tcacgccagt aaaacctcct tgattttttc
      781 actgtaggct gcgctgtctg tatctgcggt cagaaattca atcttctctg gatggtccac
      841 tcctaatccc aggtcaaccg cccttgcaat ttgctcttgc tggaatatct ttcccctggc
      901 aacctccgtc ttgcaaccga agtagcgcaa tagggcaata ccgaccgcgt caatagcgat
      961 gcgatccgtc cccgccaaaa ctacctcagg ggaggcgcgc tcgccctcat ccggcccacc
     1021 agaaataaat gcttcgatcc catccataac gatcaacgcc ggcgcgtagg ccgcgttgat
     1081 ttccgcgatc atccttctct ggttagagga aaaatgaagc tcctgcataa aatcatgatc
     1141 atctcgcggg agcttcttag ccaccatacc caccgaattc ttcagcgaca tggtgaagtg
     1201 acctccaaac ctgtgggttt tcaaacagca cgtctgcacg agcgcctctg cttcgagaca
     1261 cgggcgcgca aatgggaaac cgttttccca atgactgtct ggtggttgga tcattaccca
     1321 atcttcagct tgcagctcat caaagactat cgtcttaaat cccaattcat ctgccagccc
     1381 aaaaacgccg atcctatcca taacccggcg tgtatccccc atgccactcc ggtcgccaac
     1441 cgtaatctca gttgcaccca tctctttgag caccttcaca agcgccacga gaacttccgg
     1501 gtgggttgag cctggtgccg ggtctgagct gttgaaattc ggcttcagga aaacatgttt
     1561 ccccgcaaca ggattgatcc ctaacaggtc aatcgctctg cgcactcctt cagctcgatc
     1621 agttgttttg acgaaagcca agcgagccac gccattttcc accgattcgg tggaggttgc
     1681 agaaggaaca gcagtggcct cagttggctg gatttcttct tgcgtaggtg aagttgtcgg
     1741 taatacttcc tgcgtgagcg ttgaaatttc aagtgtatcg gtaggagaga tcaatgtctc
     1801 ctgagctatc tccgcctttg aacatccctc caggaatgaa atcagagcag ctgctccacc
     1861 cagggctgtg gtaatgcgca ggaaggccct tctactcaaa gacaaattcg attccggctt
     1921 tgccacgctt caccttcctt ccggagtaga tattcctgct accagtaaaa tttgggaaat
     1981 gcggtcaaaa taacgaatac aaaaggagcg atctgataag aggaagtatt cggacctacc
     2041 tttattatac atcctctaac tgactctaat gcccgggtgg gcgaaaatcg acgctctttt
     2101 ttaccgcctc gtcaacctcc tcgggtgtaa gcaacacgat cgtcttgata ctaaatgtac
     2161 cgctgacgtt accggcaaac gagatagcag ttgtacttac attatcaggc atatctacaa
     2221 taatgtagaa gtcgtaatca ccaaaggcaa agtagaatgg ttccagtgtt ccgccgacac
     2281 ttttcagcgc ctgttttgct gcctcgattc gactggagcc gccttcggcg aggagacctt
     2341 tcaatccttc tggagtgtac gatccatgga aaaggtattt aggctcctga cacctcctgt
     2401 caaatattat gtcttatcct atgcgaagtt tttcaagtgc cccagtacgt gctataccat
     2461 acacaaacgc tcttcgccct gcgtgggaaa tagcctctgc cattccccat aactatgctt
     2521 gaagatcttt ttcaattatc tccaaatttt gacaatgaga tactctcaaa aatgaggacg
     2581 gccgctcctg cattccgaac ggccgccccc accattgctg ttagagatta caagacagca
     2641 atactgagaa accttagtta tgcacctgca ggcggtcacc gatcagggca attcggtgat
     2701 acgcccttcg ccgccctcag gataatccgc ggctgagatc aatccaccaa ggccttcctg
     2761 gatgtaattg ctcaatgctt gctggtaggt gacaccaatg gtcgttaagg gtgccccgcg
     2821 gaaggggtac tggtcgccac ctttggctga gaaatctacg atggcgatgg tgaccccagg
     2881 accaggcacc acgactccgc ctacaacgat gactgtgcca tcatccagtt ctacatccaa
     2941 aacccgcgtg ccatccgtca cgacgttgcc gtcgccgtcg atgaccatgg cggtgccgga
     3001 ggccatccac gtgaaggaga acccagacac ctgggcgaaa cgcccgctgc cacacaacgg
     3061 gttggcaaag tcggctgtct gggtgcaagc gacggcgttt tcgaggatct ccttgaactg
     3121 ctcacggggg atatccggca ccacgctcac
//
LOCUS       DBNHOH010000660         6208 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_39588, whole genome shotgun sequence.
ACCESSION   DBNHOH010000660 DBNHOH010000000
VERSION     DBNHOH010000660.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6208)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6208)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6208
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_39588"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2230)
                     /locus_tag="AC16V7_11475"
     CDS             complement(<1..2230)
                     /locus_tag="AC16V7_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257784.1"
                     /translation="MSLLTLSSCVTLKDPETSQEHTSAVVATLEGGHSAGQSFISRRA
                     RLNGVFLWLNIREGANSDQGMLIVQLFRAPGEKTPLSAVTIPLRDAARSNPIQISLPV
                     QSDPPAQAYYLSLLATSGIVDVLGRDEDAYPYGNATRDGETLEGDFAFRTSYDYDLST
                     FWGDIRASSGHWWLIFALAIVLLLPGWLIYAILSPINVNEQSDSSDRLAIAVGLSMAF
                     IPLLMAWTSAINLPWGRLGLLFAAGMMLALMIWQLIRHKGNKPKLNWYVVILFVIFAT
                     ALIVRLIMVRDLAAPAWVDSVHHSLLTRIIMEQGGYPDNYAPYIDIDNAGYHPGFHSL
                     LASFQWLTNLNALDAMKLFGQVLNALSVIAVYLFTKSLTQDRIAGLLAAMITAYFTPM
                     PAYYASWGRYTQLAGLLILPVSFSLVKMSSAFYVNKKFVWKPLLLAAVASAGLFLTHY
                     RVVVFLGCLLLADYISRSVEEIIQNYKTNKGDSWLREVSRISLREIMGIALITLVSTI
                     LLLPWLPSALSTFIIPKAVIWQGGEQALFSGFSWAFLTTAWGKYTLYLAGLGLIWSIY
                     RKPSFAFTIILWTIFLFFLANQDALGIFPSFINNTSVEIGLFMPISALGGHIISQIVQ
                     LGWKVIPKQFKPIYIACLIVSGVLVSWIAVHQLLSILNPVTFLFRQADQPALTWISEN
                     IPQDETVLINPFNWGYGIYAGNDGGFWITPIAGIRSMPPPVFYGMDNNVDRRQGISRF
                     SQQ"
     gene            complement(2318..3410)
                     /gene="prfB"
                     /locus_tag="AC16V7_11480"
     CDS             complement(join(2318..3343,3345..3410))
                     /gene="prfB"
                     /locus_tag="AC16V7_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_158066129.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIR6257785.1"
                     /translation="MQDLIERLQTCQEKLQHLMVRLDLPQKEALLDSLQKKSEDPHLW
                     DDPENAQRVMKQMSALRDEVEGWQSLRQRLHDALDLALLEEEDLRSELETETDSLETE
                     ISRREFYAMLSGRHDRGDALLAIHAGAGGTDSQDWAEMLERMYLRWAENNSYETEILD
                     FSEGEEAGIKSVTIAVNGRYAFGYLKAEKGVHRLVRLSPFDAAHRRHTSFALVEVLPQ
                     VESDDEVEINSDDLRIDVYRSSSAGGQNVQKNATAIRITHIPTGTVVTCQNERSQSQN
                     RENAMRVLRARLLELKQREQEEQMAELRGEFKKVEWGSQIRSYVLHPYQMVKDHRTDY
                     ETGNTTAVLNGAIDNFIEAYLRSIVGNGK"
     misc_binding    3555..3881
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3939..5312
                     /locus_tag="AC16V7_11485"
     CDS             3939..5312
                     /locus_tag="AC16V7_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HIR6257786.1"
                     /translation="MSTQTRFLLNESDLPKFWYNVNADMPVAPAPVLHPQTMEPVTPD
                     FLSVLFPMDLIMQEISAERYIEIPEEVREIYKLYRPTPLLRARRLEKALDTTAHIYYK
                     YEGVSPAGSHKPNTAIAQAFYNKIGGTKALTTETGAGQWGSALALACNFFDLDLEVYM
                     VKVSYHQKPYRRIMMETYGAQVYASPTDRTQYGRSMLERDPESPGSLGMAISEAVEAA
                     ATSGGVKKYSLGSVLNHVLMHQTVIGEESLKQMDLAGEYPDVVVGCVGGGSNFAGIAY
                     PFLRQNLLEGKRTRLLAVEPNAAPSLTRGLYTFDYGDTARMAPIVKMHTLGHDFVPAP
                     IHAGGLRYHGMAPSLCALYDAGYIQAKSVPQLATFEAALLFARNEGIIPAPESAHAIR
                     AAIDEALDAKEKGEKRVILFNLSGHGNFDLSAYQAYLGGQLEDYEYPTEAIRASLKKL
                     PEVALPA"
     gene            complement(5421..>6208)
                     /locus_tag="AC16V7_11490"
     CDS             complement(5421..>6208)
                     /locus_tag="AC16V7_11490"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6257787.1"
                     /translation="LSDHAAIAISNAQLYADLQAANIAKSDFVSLVSHELKTPMTSIR
                     GYTDLLAQGAVGPINEVQTNFLNTIRSNVHRMATLVSDLADISRIEAGRMRLEFSSID
                     LSELIEEVVRTQQGQIDEKEQTLKMQIASGLPAVWGDHNRITQILVNLLSNATKYTPQ
                     GGVITLSAERTNYLQDGTALPEVVCVSVQDSGYGISPKEQKQIFQKFFRSEDENIRES
                     PGTGLGLNITRHLVEMQGGQIWFESELSKGTTFHFTIPIAATE"
BASE COUNT         1525 a         1531 c         1596 g         1556 t
ORIGIN      
        1 tctgctggct gaagcgagag attccctgtc ttcgatcgac attgttgtcc ataccgtaaa
       61 aaactggcgg aggcatactt ctaatacctg cgattggagt gatccaaaag ccgccgtcgt
      121 tgccggcata gatgccgtaa ccccaattaa acggattaat caggaccgtc tcatcttgcg
      181 ggatgttttc gctgatccac gtcagcgctg gctggtcagc ctggcggaag aggaatgtga
      241 ccggatttaa aatcgacaac agctggtgca cggctatcca tgagaccaaa acacctgata
      301 ctatcagaca cgcaatatag ataggtttaa attgtttcgg aataactttc cagccaagtt
      361 gaacgatctg gctgatgatg tgcccaccca atgcggagat gggcatgaaa aggccaatct
      421 ccactgacgt attattgatg aagcttggga agatccccaa ggcatcctga tttgccagga
      481 agaaaagaaa tatcgtccat aagatgatcg taaacgcaaa agatggcttt cgataaatgc
      541 tccaaatcaa gcccagcccg gccaggtata aggtgtactt tccccacgct gtggtcaaaa
      601 acgcccatga gaagccacta aacaatgctt gttcaccgcc ttgccaaatg actgccttag
      661 ggatgataaa tgtgctcaat gctgagggga gccagggcag gaggaggatt gtgctgacaa
      721 gtgttatcag tgcaataccc atgatttccc gcaagctgat cctgctcact tcgcgtagcc
      781 acgaatcccc tttgtttgtt ttatagtttt ggattatctc ctcaactgag cgactgatgt
      841 aatctgcaag caacaagcag cccaggaata ccaccacccg gtaatgggtc aaaaacagtc
      901 cagcgctcgc cacagcggca agcaggagtg gcttccagac gaattttttg ttcacgtaaa
      961 atgcggatga catcttgaca agggaaaacg ataccggcaa tattaataat cccgctaatt
     1021 gcgtatagcg tccccaacta gcgtaatagg cgggcatcgg cgtgaaatat gcggttatca
     1081 tggcagccag caagcctgca atgcggtctt gtgtcaatga tttggtgaac aggtatacgg
     1141 caatcacgct cagggcattc agcacctggc caaacaattt cattgcatcg agagcattta
     1201 ggttggtaag ccactgaaaa cttgccaata ggctatggaa accaggatga tagcctgcat
     1261 tatctatatc tatgtaggga gcgtagttat ccggataccc tccttgttcc ataatgatcc
     1321 gtgtgagcag gctatgatgg accgaatcca cccaggctgg tgcagccaga tcgcgcacca
     1381 tgattaaccg aactattaag gcagttgcga agatcacaaa aagaataaca acgtaccaat
     1441 ttaatttggg tttgttgcct ttatgccgta tcaattgcca gatcatcaaa gccagcatca
     1501 tgcccgcggc gaatagtagc cccaacctac cccagggaag atttatcgct gatgtccagg
     1561 ccataagcaa tgggatgaaa gccatgctga ggcctactgc aattgccagc ctgtcactgg
     1621 agtcggattg ttcattgaca tttatagggg aaaggatggc gtagattaac cagccgggaa
     1681 ggagtagtac gattgctagc gcgaatatta accaccagtg accagatgac gcgcggatgt
     1741 caccccaaaa agtgctcaag tcataatcat agcttgtccg gaatgcgaaa tctccctcca
     1801 gcgtttctcc atctcgggta gcgttcccat acgggtaagc atcctcatca cggccaagca
     1861 cgtcaacgat tcccgaagtg gctaacagtg atagataata ggcttgcgcc ggcggatcgg
     1921 attgtacagg tagactgatc tgtatcgggt tggagcgggc tgcgtcgcgc agggggattg
     1981 tcacggctga tagaggtgtt ttttcgcctg gtgcgcgaaa taattgaact atgagcatcc
     2041 cctggtcgct atttgcaccc tctcggatgt tcagccataa gaaaacacca ttcaggcgag
     2101 cccggcgtga gatgaaggat tgaccagcgc tgtgaccgcc ttctagagta gcaacgacag
     2161 cgctggtatg ctcttgagat gtttctgggt ctttgagggt aacgcagctc gagagggtga
     2221 gcagggacaa aaagctgaaa aacagaagca aaattctctg actcgatctg gctttgatcg
     2281 tcaacgctgg cctctgtgtt acgggatttc ctgctcgcta tttcccattt cccacgatgg
     2341 agcgtaggta ggcttcgatg aagttgtcaa tggcaccgtt gagcacagcg gtcgtattcc
     2401 cggtttcgta atcggtgcga tgatccttaa ccatctgata agggtgaagc acgtatgagc
     2461 ggatctggct gccccattct actttcttga attcgccgcg cagttcagcc atttgctctt
     2521 cctgttctct ttgttttaat tccaataagc gagcgcgcag tacgcgcatg gcgttttctc
     2581 ggttttgaga ctgggagcgt tcattctggc aggtgactac ggtaccggtg ggaatgtggg
     2641 taatacggat tgcagtggca tttttttgca cattttgccc gccagcgctg gatgagcgat
     2701 atacgtcaat tcgtaaatcg tcagagttta tctccacctc gtcgtcactc tccacctgcg
     2761 ggaggacctc caccaaggca aaggaggtat gcctgcggtg agcagcatcg aagggggaga
     2821 ggcgaaccag gcggtgcacc cccttctcgg ctttgagata accgaaagca tagcgcccgt
     2881 taacagcgat agtaacactc ttgatcccgg cttcttctcc ctcgctaaaa tccagaatct
     2941 cagtctcgta gctattgttc tctgcccagc gcaagtacat acgctctaac atctctgccc
     3001 aatcttgcga gtcggtaccg ccagcaccgg catgaatggc aagcagcgcg tcgccacggt
     3061 catgcctgcc tgaaagcatg gcgtaaaact cgcgccttga gatttcggtt tcaagtgagt
     3121 ctgtttccgt ttcgagctca cttctcaggt cttcttcttc gagcagggct agatctaacg
     3181 cgtcgtgtag tcgctggcgc agcgattgcc aaccctcgac ttcatctcga agggcagaca
     3241 tttgtttcat cacgcgctgc gcattctctg ggtcatccca aagatgcggg tcttcggatt
     3301 ttttttgaag ggaatctaac agggcttctt tttggggtaa gtcaaagacg caccatgagg
     3361 tgctgaagtt tttcctggca ggtttgcagg cgttcaatca ggtcttgcat acaataatac
     3421 tccgatcgta attttcctta ttttacatca tttcggcgat gttcgcaggc ttcgaacttg
     3481 tgaaagctat tttgatgttg acgcaaaacc ttgacaacaa gttcttttag tatataatcc
     3541 acccaactgt aaattaaaag acgttgaaga ggaatagtac gcctcaacac ggcagtccag
     3601 agagcaggga tcgggtgaga gccctgtctg tacgctgaag cggaatggtc ctcggagttg
     3661 ccaggtgaaa agcgtactgc ttaatagcct gcgccggaac tgccgccgtt atccaggcat
     3721 aggtataact agtagccatg ttaatcggtt actggcgtac cgtaacgagt gaggctggcc
     3781 tccagttgat caatccccgt cgcatcatcg acgggttttt atttcaacca aaggctgcct
     3841 aaccggggtg gcaccacgga catagcgcgt tcgtccctta gagggatggg cgcgtttttt
     3901 gttcttcatt ttctactcac tttcctaagg aggcaaccat gtctactcaa acccgttttt
     3961 tgctcaatga atccgaccta ccaaagttct ggtacaacgt taacgccgat atgcctgtag
     4021 ccccggctcc tgtcttgcat ccccagacaa tggaaccagt aacgcctgat tttctgtccg
     4081 tgcttttccc aatggattta atcatgcaag agataagcgc tgagcgatac attgaaattc
     4141 ccgaagaggt gcgcgagatt tataaactct accgaccaac acctctattg cgcgcccgcc
     4201 gcttggagaa ggcattagat acaactgcgc atatatacta taaatacgaa ggagtatccc
     4261 cagcgggtag ccacaagcca aatacagcaa tcgcacaagc cttctacaat aaaatcggag
     4321 gtaccaaagc cttgacgact gaaacaggcg ctgggcaatg gggctcggca ttggcgctgg
     4381 cgtgcaattt ctttgatttg gatttagaag tctatatggt gaaagtctct taccatcaga
     4441 aaccgtatcg caggatcatg atggagactt acggcgcgca ggtatatgct agcccaaccg
     4501 atcgcacgca atacgggcgc tccatgttgg agcgagatcc agagagcccc gggtctttgg
     4561 gcatggcgat ctctgaagcg gttgaggctg ccgccacttc aggcggagtg aagaaataca
     4621 gcctgggttc ggtattgaac catgtcttga tgcatcagac cgtcatcggc gaagagtctc
     4681 ttaagcagat ggatctggca ggtgaatatc ccgatgtggt ggtcggctgt gttggaggcg
     4741 gatcaaattt cgctggtatc gcctatccct tcttgcgcca gaacttactc gaaggaaaac
     4801 gcacccgctt attagctgtt gagcccaatg ctgcgccttc gctgaccaga ggtttgtata
     4861 catttgatta cggagatact gcccgcatgg ccccaatcgt caagatgcac accttggggc
     4921 atgactttgt tccagcgcca attcatgcgg gtggtttgcg ctaccacggt atggctccca
     4981 gcttatgcgc cttatatgat gccgggtata ttcaagcgaa atcggtaccc caactggcta
     5041 cattcgaggc agctttgctc tttgcacgaa atgaagggat catacctgct ccggagagcg
     5101 cgcacgccat ccgggcggct atcgatgaag ctctagatgc caaggaaaaa ggcgagaagc
     5161 gcgtaatctt atttaacctt tcgggtcacg gcaacttcga cctttctgct tatcaggcat
     5221 acctgggtgg tcagctggag gattacgagt atcctaccga ggcaattcga gcctcgctga
     5281 agaagctgcc agaggtggct ctcccagcct agttatctat acgagcggta agtacacatt
     5341 ccgaatcctg gtcccgcctg attggggcgg gactgggata tggggaaacc ataacgtagc
     5401 tagacgcctg gatcggacta ttattcagtg gctgcaatgg ggattgtgaa atggaaagtt
     5461 gtgcccttgg ataattcgct ctcgaaccaa atttgcccgc cttgcatttc caccagatgg
     5521 cgggtgatgt tcaagcctaa gccggttcca ggggattcac gaatattctc gtcctcagag
     5581 cggaaaaact tttgaaatat ctgtttctgc tctttcggtg agatgccgta accactgtcc
     5641 tgaactgaca cgcacaccac ctctgggagg gctgttccgt cctgtaaata atttgttctc
     5701 tcggcgctca gagtgatcac gccaccctgc ggggtgtatt tggtggcgtt gctcaagaga
     5761 ttcactagaa tctgggtaat acggttatgg tctccccata ctgcaggcag ccctgatgca
     5821 atttgcatct tgagggtttg ctccttttcg tcaatctgcc cctgttgggt gcggacgact
     5881 tcttcaatca attcggacag gtcaatggaa ctgaattcaa gtcgcatgcg gcctgcttca
     5941 atgcgcgaga tgtcagctaa atcggaaacc agggttgcca tacgatggac atttgagcgg
     6001 atcgtattta gaaaattcgt ctgtacctcg ttgattggac ctacagcacc ttgagccagt
     6061 aaatcagtgt agccccggat agatgtcatt ggtgtcttaa gctcgtgtga aaccagggaa
     6121 acaaagtcgc tcttggcgat attggcagct tgtagatcgg catagagttg ggcgttggag
     6181 atggctatag cggcatggtc gcttagac
//
LOCUS       DBNHOH010000661         1958 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1066116, whole genome shotgun sequence.
ACCESSION   DBNHOH010000661 DBNHOH010000000
VERSION     DBNHOH010000661.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1066116"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="AC16V7_11495"
     CDS             <1..971
                     /locus_tag="AC16V7_11495"
                     /inference="COORDINATES: protein motif:HMM:NF047050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6544 family protein"
                     /protein_id="HIR6257788.1"
                     /translation="EVGVAGMRVFSLLWLLATLGFVIAAIALALGKSNWAPLMLGAAL
                     LSIVLCTLDWSAAFRGALIDVFFLLVLAMVFGLRMQPSPFPSYTGVPAPLITSPIPAG
                     LPEPVERFYRMHHGDEVPEYHSVVVSMRGTLRFMGITFPARLRFSHIAGQDYRHYIEA
                     TFYGYPVMKVNEHYLGGHSRLELPFGVVENDPKVDSAANQGLWAETFAFFPAVILTDG
                     RVRWEPVDENTAKAYVPFGESEQVFTVQFDPQTGYLRGMETLRYRDQKVGTIRWRTEL
                     MRGENSNEPIQISATWEDEATPWMVVEIEETVFNTDLTEYILQTGQ"
     gene            complement(1026..>1958)
                     /gene="holB"
                     /locus_tag="AC16V7_11500"
     CDS             complement(1026..>1958)
                     /gene="holB"
                     /locus_tag="AC16V7_11500"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HIR6257789.1"
                     /translation="RHAYLFTGPQGVGRRTLAVRLAQALNCPQPPAPGVPCRTCRTCT
                     RLEAMQHPDLAIVQAEQVGGTLKVDQVRELQHSLSLAPYEARYRVALLLRFEEAHVSA
                     ANALLKTLEEPPPQVVLILTAENAENLLPTIVSRCEVLRLRPLTAEMISQGLQDRAGL
                     SPDQASLIAHLSGGRPGYALHLSQQPGELELRQEWLNDHQRLLAASRVDRFTFSESRS
                     KDKELLRQILMVWLSLWRDVLLQASGASIAHTNLALGAEIEKMASDVGLQGASAMVAS
                     LQRTLDLIDRNVNARLALDVLLLDLPRMNSTEIR"
BASE COUNT          402 a          527 c          603 g          426 t
ORIGIN      
        1 tggaggtggg cgtggcggga atgcgggttt tcagcttatt gtggttgctg gcgacgcttg
       61 gatttgtgat cgcagctatt gcactagccc ttgggaaatc gaattgggcg ccgctgatgc
      121 tgggcgcagc ccttttatcg atcgtactgt gtacgttgga ttggagtgca gccttccgcg
      181 gcgcgctgat cgatgtcttt ttcttgctgg tgctggcgat ggtgttcggc ctgcgcatgc
      241 aaccgtctcc ctttccgtca tataccggcg tacccgctcc gctgataaca agccccattc
      301 ctgctgggtt accagaacct gtggagcgct tttaccgtat gcatcatggc gatgaagtgc
      361 cagagtacca ttctgtggtc gtcagcatgc gcggcacgct gcgcttcatg ggaatcacct
      421 tcccagctcg attacgtttc agccacatag ccggtcagga ttaccgccat tacatcgaag
      481 ccacctttta cggttaccca gtgatgaaag tgaacgagca ctacctgggc ggtcacagcc
      541 ggcttgaatt gccctttggg gtagtggaaa atgatcccaa ggtggacagc gcagcaaacc
      601 agggcttatg ggcggaaacc tttgcatttt ttccggcggt aatactcacc gatgggcgcg
      661 tgcgctggga gcccgtggat gagaacactg caaaagccta tgtgcccttt ggggaaagcg
      721 aacaggtctt cacggtacag ttcgacccgc agactggata cctgcgcggg atggaaaccc
      781 tacgctatcg agatcagaaa gtcggcacaa tccgctggcg gacagagctg atgcgggggg
      841 agaattcaaa tgagccgatt caaatctcag ccacctggga ggatgaagct accccctgga
      901 tggtggttga aatcgaagag acggtattca acaccgactt aaccgaatac atccttcaga
      961 ccggtcagtg atcgaatcgg ggttttccca aaggctttcg actttaggaa aaccctatta
     1021 acggatcaac ggatttctgt tgaattcata cgcggcaggt cgagcaagag aacatccaat
     1081 gctagacggg cgttgacgtt gcggtcgatc agatccaggg tgcgctgcag ggaagcgacc
     1141 attgccgagg cgccttgcaa gcccacgtca gacgccattt tctcgatttc tgctcccaat
     1201 gctaagttgg tatgagcgat agaggcgcct gaggcttgta gcagaacgtc gcgccatagg
     1261 gagagccaga ccatcagaat ctggcgcaac agttctttgt cctttgagcg actctccgaa
     1321 aatgtgaagc gatctacgcg gctggctgcc agcaggcgct ggtgatcgtt cagccattcc
     1381 tggcgaagct ccagctcgcc aggctgttgg ctcagatgca aggcataacc cgggcgcccg
     1441 ccggataggt gggcgatcag gctcgcctga tcaggtgata gcccagcgcg atcctgcagg
     1501 ccctggctga tcatctccgc tgtcaagggg cgcaaacgca atacttcgca gcgcgagacg
     1561 atggtgggta gcaggttttc ggcgttttct gcggtgagaa tgagcaccac ctgaggcgga
     1621 ggttcttcca gggttttgag cagggcgtta gcggcgctga cgtgggcttc ttcaaagcgc
     1681 aacagcagcg ctacccggta gcgcgcttcg tatggagcca gggagaggct gtgttgcagc
     1741 tcgcgtacct ggtctacctt gagcgtacct cccacttgtt cagcctggac aatcgccagg
     1801 tcgggatgct gcatggcttc caggcgcgtg caggtccggc atgtgcggca gggcacgcct
     1861 ggggcaggcg gctgcgggca gttcaacgcc tgggcaagac gcacagccag cgtgcgccgc
     1921 cccacgccct gcggtccggt gaagagataa gcatggcg
//
LOCUS       DBNHOH010000662         2631 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_424382, whole genome shotgun sequence.
ACCESSION   DBNHOH010000662 DBNHOH010000000
VERSION     DBNHOH010000662.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_424382"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="AC16V7_11505"
     CDS             complement(<1..729)
                     /locus_tag="AC16V7_11505"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR6257790.1"
                     /translation="MSSKRYIWWWVLPAAILFLVIILVPFVTALTYSFRALSFTDSTA
                     AGQYIGADNYRKLVFDKDFYNSFKVTFLYMFPAVIVQGLLGLAMSLLISGVVRRARIL
                     IPIMLLPTLLTPVVIGLVGVLTLNPEFGVIGQWLNRSGLIKEAVLGSGKWALPAIIAV
                     DTYQWTPFVAVILTSGLLALPKEPYEAAQVDGANRVQTFFRVTMPLLWPYFVVALLIR
                     VIDAFRVFDIIWVMTRGGPGTVTET"
     gene            complement(761..1876)
                     /locus_tag="AC16V7_11510"
     CDS             complement(761..1876)
                     /locus_tag="AC16V7_11510"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6257791.1"
                     /translation="MASVIIENLFKQYKGGEIQALKGVNLTCDDGEFFCILGPAGAGK
                     TTLIKLIAGIEAADDGNIRIGNQDATDKLPMDRDIAVAFETYALYPQRTVRENLAFPL
                     QAPSRRNEWTKEAIETRVVEIANILGIGELLERYPRQLSGGQRQRVALGRALVRKPQA
                     YLLDEPIAHLDAKLRHRMRGELKRIQMELGITTIYTTPDQLEALSLADKMVVLNQGKI
                     EQFGTPTEIYKYPANVFVARFVGDPPMNILDANLDADGITFQGLGRLPSNETSKKVLK
                     HSANNGNILIGIRPRDLELLPSSAEKSWVTAEVRHVQILGETCIVSFNAGPFDLRSRL
                     ATSQAPERGQKVGVFIDPETFHYFDHQSHQRIDNHVN"
     gene            complement(1909..>2631)
                     /locus_tag="AC16V7_11515"
     CDS             complement(1909..>2631)
                     /locus_tag="AC16V7_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008385377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6257792.1"
                     /translation="LLQMRVRGLSGGQMQSVGIGRAIVREPSILLMDEPISHLEARQR
                     ARMREELVDLQKRLGTTSIYVTHDQVEAMTMATRIAVMNLGELQQVGTPKEVYEQPQT
                     EFVGGFIGEPPMNFTDCDIRQENGSYFAESPSFRVPLGEALGTKLKSYDGSRSIRMGI
                     RPESIHVSMEEMADSFQASVYVLEPQGERSILTARLSQGEFFLVEVPPNFEADPDTVI
                     YMQFQEPIHLFDKANGRNLFYV"
BASE COUNT          672 a          660 c          630 g          669 t
ORIGIN      
        1 agtttcggta actgtccctg gacctccgcg tgtcattacc cagatgatgt caaacacgcg
       61 aaatgcatca attacgcgga tgagcaacgc aacaacaaaa taaggccaaa gaagcggcat
      121 ggttacacgg aaaaatgttt gaacgcggtt tgcgccatcc acctgagcag cctcgtatgg
      181 ctcctttggc aaggcaagca atccagaggt gaggataacc gccacaaagg gtgtccattg
      241 ataggtgtcc acagcgatta tcgcaggtag agcccacttc ccgctgccca agacagcttc
      301 cttgatcaat ccagagcggt tcaaccactg gccaataacg ccaaattcag ggttcagcgt
      361 gagaacgcca accagcccga taactacagg ggtcaacagc gtgggaagaa gcatgatagg
      421 gatgagaatc cttgcccgcc gcacgactcc agagattaac agcgacatgg ctaaccccaa
      481 taacccctga acgatcactg ccggaaacat atacaggaag gttaccttga agctgttgta
      541 aaaatcctta tcaaagacta acttccggta gttgtcggca ccgatgtact gaccggctgc
      601 tgtggaatcc gtaaagctaa gagcacggaa actgtaggtc agggcggtga caaaaggtac
      661 taagataatc accaggaata aaattgccgc aggtaatacc caccaccaaa tgtaacgttt
      721 gctgctcata tgttagctcc tcttctacaa agatggtgcg ctagttaaca tgattgtcaa
      781 tccgctggtg ggattgatga tcgaaatagt gaaatgtctc tgggtctatg aaaaccccaa
      841 ccttctgacc ccgttcagga gcctggctag tggctaagcg tgagcgtaaa tcaaatggtc
      901 cagcattaaa cgaaacgatg catgtctctc caagtatttg aacgtgtctt acttctgctg
      961 ttacccaact tttctctgcg ctcgagggca atagctcaag gtccctggga cggataccaa
     1021 ttaatatatt tccattgttg gcggaatgtt tcaaaacttt tttcgaagtt tcatttgatg
     1081 gcaggcggcc aagcccctgg aaggtgatcc catccgcatc cagattagca tccaatatat
     1141 tcattggagg atcgccaaca aaacgagcaa cgaagacatt ggctggatat ttatagattt
     1201 cagtgggggt accgaattgc tcaatttttc cttggttaag tacaaccatc ttgtctgcca
     1261 gagataaggc ttccaattga tctggggttg tgtagatcgt ggtgatacct aattccattt
     1321 gaatgcgttt gagctcgcca cgcatgcgat ggcgcaattt tgcatctaaa tgagcgatgg
     1381 gctcatcgag aagatatgcc tgaggtttgc gaaccagggc gcgccctaaa gctacccgct
     1441 gtcgttgccc gcccgataat tggcgcgggt agcgctcaag tagctcacca atccccagga
     1501 tattggcaat ctctaccacg cgtgtttcta tagcctcctt tgtccactca tttctgcgtg
     1561 aaggtgcttg taaaggaaac gcaagattct cgcgtacagt gcgttgtggg tatagggcat
     1621 aggtttcaaa agccaccgcg atgtcccggt ccatgggtaa tttgtcggta gcatcttgat
     1681 tgccgatcct gatattgcca tcatccgctg cttcaatgcc tgcaatcagc ttaattaagg
     1741 tggttttccc agcaccagca ggtcctaata tacaaaaaaa ttcgccatca tcgcaggtca
     1801 ggttgacacc ttttagcgcc tggatttcgc cacctttata ttgcttaaat aagttctcta
     1861 taataacgct tgccatctaa tcctccgtgc catacggaat gagcctgctc agacgtagaa
     1921 caggtttctt ccattggctt tgtcgaataa gtggatcggc tcttgaaatt gcatgtagat
     1981 aaccgtatct gggtcagcct cgaaatttgg agggacttcg accaggaaaa attcgccttg
     2041 actcaagcga gccgtcagaa tggatcgttc cccttggggt tctaagacat aaacgctggc
     2101 ttggaaacta tcggccattt cttccatgct gacatgaata ctctctggac gaatgcccat
     2161 ccggatggac ctgctgccat cataagattt tagtttcgtc cccaatgcct cgcctagagg
     2221 gactctgaag gatggcgact cggcaaaata actgccattc tcctggcgga tatcacaatc
     2281 cgtgaagttc attggtggct cgccaataaa gccgccgaca aattcggtct ggggttgctc
     2341 gtatacctct ttgggtgtgc ccacctgttg taattcgcct aaattcatga cggcgatgcg
     2401 cgtcgccatt gtcatggctt ccacttgatc gtgagttacg tagatagagg ttgtcccaag
     2461 tcgcttttgc agatcgacca attcttcgcg catgcgagcg cgttgccgtg cttctaagtg
     2521 tgagatgggc tcgtccatta acaggatcga tggttcacgc acgatagccc gtccaatgcc
     2581 tacgctttgc atttgaccgc cgctcaatcc gcgaacccgc atttgcaaca g
//
LOCUS       DBNHOH010000663         3043 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1258058, whole genome shotgun sequence.
ACCESSION   DBNHOH010000663 DBNHOH010000000
VERSION     DBNHOH010000663.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1258058"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="AC16V7_11520"
     CDS             complement(<1..1259)
                     /locus_tag="AC16V7_11520"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6257793.1"
                     /translation="MNEKVGVYICHCGTNIAGTVDVMEVARWASEQPQVAIAREYKFM
                     CSSLGQELIENDIKEMGLTRVVVAACSPHMHEKTFRSACQRAGLNPFLFEMANIRELD
                     SWITDDKEAATQKAKAMIKGAVERVLHHQPLNYLPVNINPHTLVVGGGIAGITSALEL
                     ANAGHHVYLVERDPSIGGHMAQLDKTFPTLDCAACILTPRMVEVGQHPNITLLSYSEV
                     ENIEGYMGNFNVTVRKKARYINEELCTGCAVCEEKCPKKVLDEDFNVGLGYRKAVYRP
                     FPQSVPMYPVIDVQNCTYFIKGKCKACEIFCPAGGIDFDQKDEQLELQVGSIILATGY
                     SPFDARRIPQYGYGRLPNVFTSVEFERLVNASGPSAGRIVLRDGVTTPESVAIIHCVG
                     SRDQNYNEYCSRVCCMYSLKFAHLVRER"
     gene            complement(1274..>3043)
                     /locus_tag="AC16V7_11525"
     CDS             complement(1274..>3043)
                     /locus_tag="AC16V7_11525"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HIR6257794.1"
                     /translation="IYAGVMHLTIFWGMVVLFLGTVVATVDWDVTHLFFGFQFLTGGI
                     YVIYELVLDIFGILLLVGLGMALYRRYIVRPGRLQNMPAGRLAWDDAYVLTMLVLITI
                     SGYLIEGLRIAVIRPDWAAWSPVGSAIASAFLALGDPTSRSLHLSLWTAHGLLSFMFI
                     ASVPYTKLFHLISTPVNIFFQSTKPVGALVPVRYSSGPGVQEWKNFTWKQILDFEACT
                     RCGRCHDACSALGSCGLLSPRDLMIKLDQHVWERQNGKSLHGDVLSPEEIWACTTCRA
                     CSQVCPAYIDHVTTLVDLRRHLVDEGEMDGLLQEALTNLGRYGNSFGQSERMRARWTA
                     GLEPAIKDIRKEPAEYLWFVGDYASYHAGVRDITRKTAEVFQKAGIDFGILYEAERNS
                     GNDVRRVGEEGLFEMLVEKNLAVLDRCDYQSVVTTDPHSYNTLKNEYSTNGNGRHPVL
                     HYSELLDRLLTAGQLKLSRKLDYTVTYHDPCYLGRYNGVYDAPRRVISATGCKLVEMP
                     RHGDRALCCGAGGGRIWMEEGPVLERSSEARIHEAVGQNGVQVLVTACPKDVAMYTDA
                     VKTTGFADRLMVKELVELVHEAL"
BASE COUNT          760 a          877 c          732 g          674 t
ORIGIN      
        1 cgctcgcgaa ccaggtgggc aaacttcaaa gaatacatgc agcatacgcg ggaacagtac
       61 tcgttataat tctggtcacg tgagcccacg cagtggatga tcgctacact ttcaggcgtg
      121 gtcactccat ctcgcaagac gatccttcca gcggatggcc cagatgcgtt caccaggcgc
      181 tcaaattcga cgctggtaaa gacgttcggc aggcgaccat aaccatattg agggatgcgg
      241 cgggcatcga acgggctata tccagtggcc aggataatgc ttcccacctg tagctccagc
      301 tgctcatcct tctgatcgaa gtcaatcccg ccagcaggac agaagatctc gcacgcctta
      361 cacttcccct tgataaagta cgtacaattc tgcacgtcaa tgaccgggta catgggaaca
      421 gactgaggaa acgggcggta tactgcctta cgatacccca ggccaacatt gaagtcttcg
      481 tccagcacct tcttagggca cttctcttca cataccgcgc agcctgtgca caattcttca
      541 tttatgtaac gggctttctt acgtacagtg acgttgaagt tgcccatata cccttcaata
      601 ttttccacct cgctgtaact taacaaggtg atattgggat gttgccccac ttccaccatg
      661 cgaggtgtca ggatgcaggc agcgcagtcc agtgtaggaa aggtcttatc gagttgagcc
      721 atatgaccgc caatcgaagg atcgcgctcc accaaataga catggtgccc cgcattggct
      781 aattccaaag cagatgtaat gccggcgatg cccccgccca caaccagggt atggggattg
      841 atattaaccg gcaagtaatt aagcggctgg tggtgtaaca cacgttccac agcgccctta
      901 atcattgctt tagccttttg ggtggcggcc tctttatcat ccgtgatcca cgaatccagc
      961 tcacggatat tcgccatttc aaacaaaaac gggttcaaac cagcccgctg gcaagcgctg
     1021 cgaaaagttt tctcgtgcat gtggggtgaa caggccgcca ccacgacgcg agtaagcccc
     1081 atttctttaa tatcattctc aatcagctct tggcctaggc tggagcacat gaatttgtac
     1141 tcgcgggcaa tggctacctg gggttgctcg ctggcccaac gtgcaacctc catcacatcc
     1201 accgtcccag cgatatttgt tccacaatgg cagatgtata cgcctacttt ttcattcatc
     1261 gctgccctcc tgtttagagc gcttcgtgta ccagctcaac taattccttg accatcagac
     1321 gatctgcgaa gccagtggtt ttgaccgcgt ctgtgtacat ggcgacatct ttggggcaag
     1381 ccgttaccag cacctgcaca ccattctgcc ccacggcttc atggatgcgg gcttcgcttg
     1441 agcgttctag caccggtcct tcttccatcc aaatacgccc accaccagcc ccacagcaca
     1501 atgcccggtc gccatgacgt ggcatttcta ccagcttgca gccagttgca gatatcactc
     1561 ggcgcggcgc atcatataca ccgttgtaac gaccgagata gcatgggtca tgataggtga
     1621 ccgtataatc cagttttcgg ctcagcttga gctgccccgc agtgagcaaa cgatcaagca
     1681 gttcgctgta atgcaatact ggatgccgcc cattcccatt cgtcgaatac tcattcttga
     1741 gtgtgttgta gctgtgcggg tcagtggtca cgacagattg ataatcgcac cggtcgagta
     1801 cagccaggtt cttctcgacc agcatttcaa ataggccctc ctcacccaca cgacgcacat
     1861 cattgcctga attgcgttcg gcttcgtaga gaataccgaa atctatgcct gctttttgga
     1921 atacttcagc ggtctttcgc gtgatgtccc tcacgccggc atggtaagaa gcgtaatcgc
     1981 ccacaaacca caaatattct gctggctctt tacggatatc cttgatcgca ggctccaaac
     2041 ctgccgtcca gcgggcgcgc atgcgctccg attgaccaaa ggagttgcca tagcggccaa
     2101 ggttggtcaa ggcttcctgt aacaggccat ccatctcgcc ttcatccacc aaatggcggc
     2161 gcaggtctac tagggtagtc acatggtcaa tataagccgg gcagacctgg gaacaggccc
     2221 ggcaagtggt gcatgcccat atctcctcgg gtgagagcac atcaccatgc aggcttttac
     2281 cattttgccg ctcccatacg tgctgatcca gcttaatcat cagatcgcgc ggggagagca
     2341 atccacaact gcccaaggcc gagcaagcgt catggcagcg cccgcagcgg gtgcaggcct
     2401 caaaatccag aatctgcttc caagtgaagt tcttccattc ttgaacaccc ggtccggagg
     2461 aatagcgtac tggcaccagc gcaccaaccg gctttgtgga ctggaagaag atgttgactg
     2521 gtgtcgatat caaatgaaag agtttggtat aaggaacgct ggcaataaac atgaaagaca
     2581 gcaagccgtg cgcagtccag aggctaaggt gcaggctgcg actcgttggg tcacctaaag
     2641 ccagaaacgc agaagcaatt gcactgccaa ccggagacca ggcggcccaa tccggtctga
     2701 tgacggctat acgcaacccc tctatcagat atccactgat cgtgatcaac actaacatgg
     2761 taagcacata ggcgtcatcc caggccaggc gacctgcagg catgttttgc aagcgaccag
     2821 gacggacaat atatcgcctg tatagggcca tccccagtcc aaccaacaac aagataccga
     2881 aaatgtccag aaccaactcg taaatcacat aaatgccacc tgtcagaaac tggaagccaa
     2941 aaaacaggtg ggttacatcc caatctactg tcgccaccac agtgccaagg aaaagcacaa
     3001 ccatacccca gaagatggtg agatgcatga ccccggcata gat
//
LOCUS       DBNHOH010000664         4539 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_809168, whole genome shotgun sequence.
ACCESSION   DBNHOH010000664 DBNHOH010000000
VERSION     DBNHOH010000664.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_809168"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..603)
                     /locus_tag="AC16V7_11530"
     CDS             complement(106..603)
                     /locus_tag="AC16V7_11530"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HIR6257795.1"
                     /translation="MSSVTRICFVCLGNIVRSPLAKSLFLYHANQAGRGDLYEVDSAA
                     TSGWHVGESPDPRMRRVSARHGVPLDGRARQFKKPDFERFDLILVMDGENYSNLRRLG
                     PSPEQLQKVHFLRDFEPSGGSRLEVPDPYYAGIDGFETVFEIVDRSVRGLLDVLENNT
                     PVKRR"
     gene            complement(912..4295)
                     /locus_tag="AC16V7_11535"
     CDS             complement(912..4295)
                     /locus_tag="AC16V7_11535"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6257796.1"
                     /translation="MEKLFGLEMNMIAGCLGSSLALVIVVLALLALRERVMFKLGIRP
                     IARRRTQSALIVFGLMLATLIITAAFFMGDTMSHTIRSLTLDELGEVDEIIRLSGAGS
                     GTTQQTYFKFARYEELVTALEDFEMIDFFVPAVRESIPVVNTTRRKSERSLTVIGLRQ
                     SDTAILENEELIDVEGKPLNLTQLGPREVYLNAAAAQDLNASPGDKLELYTESKPKIV
                     QVRSVTAGGKSPRMFLPLRRAQTLFKQRGRINEIFISNIGDASTGANLSQPVTTHLRG
                     LLTDRVVAGRIVNLLLRDASILGTLRGEASDLRGNIQLDLLALTDFLETGVLSNELLS
                     LLADEEVADEVQVILDQAEWMNQPSRDRLAELFVDLSELEVVDVKRDNLDQGELAASA
                     FTTIFIVTGLFGIAAGLLLIFLIFVMLAAERKPEMGMTRAIGGQRMHLVEMFVFEGTA
                     YDLGAAAVGVGLGIVTGYVLAASLGQAFAAQSDLAIRPNLTIRSMIVSYSLGMLVTFG
                     TVLFSAYKVSKLNIVAAVRDLPEPPSPPTRLRDRLFAPFRVLVEGFRHLRRLRIGRAL
                     WTFWVQAPLSLLGVVWAGLIGGPLTLLVGVILTPAGVAQSSGAAFSLGGSFLIIGVCL
                     TLRGLLSLALRKRPGLADRLAFTLLGLGLVIFWSLPSGFFEPLGVKDLKSGPEMLFIS
                     GILLVAGAVLLVMYNTDLLLKIILRLTGGARRLTPVLRMSIAYPLANRFRTGLTMGMF
                     AVVVFSVIFMATAFKVNETFFADTDALTGGFDLRTESSRTNPVPDLEAAIASNRDINQ
                     RDFLVVADRSSLGIEARQGRGQWRDYLIYGVDDDYLENVAFDLTVMAEGYKSGAEVWR
                     AVRDERGYAVIDSFGVPSRQTTSIVIGAPDFKLEGVYIEDESMQPITIEVRDSFSEAT
                     FEVTVIGVLEPGANLGLYTSQRTLERELPYELVSTTHFIRLAGGVDRAAAGAALEGAF
                     VKHGLQSIDQIEELKDAQASQRVIEVLLQGFLTLGLVVGVAALGVVSTRAVVERRQQI
                     GMLRALGFQRQMVSWSFMIESSFVALLGISMGTVLALIPAYHMINDMAQEYPSVKFQV
                     PWNSIIVVVGLAYGMALLATWLPARQASRVAPAEALRYE"
     gene            complement(4298..>4539)
                     /locus_tag="AC16V7_11540"
     CDS             complement(4298..>4539)
                     /locus_tag="AC16V7_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6257797.1"
                     /translation="ERQRTTIARALANRPAIVWADEPTGDLDSETATDIMDLLLRLNV
                     EAGLTFILVTHDRQVGARCGRMVRMRDGLIENETS"
BASE COUNT         1119 a         1346 c         1157 g          917 t
ORIGIN      
        1 aagcttgcgc tttcaaccag tcgctcacag gctcaggaag catattcacc atccaattta
       61 cattgagtag tacagcaggt catttcggcc aatgtcacgg aaagtttatc tgcgcttgac
      121 cggggtattg ttctccaata catccaataa tccacgcacc gagcgatcaa cgatctcaaa
      181 aaccgtctcg aacccatcga taccagcgta atatggatca ggaacttcca atcgcgatcc
      241 gcccgagggc tcgaaatcgc gcaagaaatg tactttctgc aattgctctg gggagggacc
      301 cagtcggcgc aggttgctgt aattttcgcc atccatgacc aggatcaggt caaaacgctc
      361 gaaatcaggc tttttaaact ggcgagcgcg accatcaagc ggcacaccat gccgggcaga
      421 cacccggcgc atgcgtgggt cgggcgactc gccgacatgc cagcccgaag tggcagccga
      481 atcgacctca taaagatctc ctctacccgc ctgattggca tggtaaagaa acaagctttt
      541 cgccagcggg ctgcgcacga tattgcccag acagacaaaa caaatccgtg taacagacga
      601 caaatgtaac ctccaaaaac aaacaattta cgcaccattg tacccgaaat taaataacgg
      661 gcgattatgc cgcccgctaa tattttcggg ataaattctc catttgatct aattaaataa
      721 ccgcaagagc gataaatcgc gccgacgcga tagaactgca accttatgaa ctaaagtttt
      781 ttctggaacc gaaccgcaaa tgcacccgcc ccatcacagc taccgcctgg ccattagctg
      841 ttgaatttct ttcattattc tattggaata tatctcccaa tgttagtact atgtagaaag
      901 catcgcaagc tttactcgta gcgcaaggct tccgcaggcg ccacgcgaga tgcctgacgg
      961 gcaggcagcc atgtcgccag cagagccatc ccataggcga ggccaaccac cacgataata
     1021 gaattccagg gtacctggaa ctttacactg gggtactctt gcgccatatc gttgatcata
     1081 tgataggctg ggattagcgc cagcaccgtg cccatactga tacccagcaa cgccacgaaa
     1141 gatgactcaa tcatgaaact ccagctgacc atctggcgct gaaagcctag cgctcgcaac
     1201 atcccgattt gctgacgacg ctccaccact gcccgggtgc taaccacccc cagtgcagct
     1261 acacccacca ccagtcccaa agttaagaac ccctggagca agacttcgat tacgcgctgg
     1321 ctggcctgcg cgtctttcaa ttcttcgatc tggtcaatgc tctgcaggcc gtgtttcaca
     1381 aatgcgcctt ccagcgcagc ccctgctgcc gcccgatcaa cacccccagc cagccggata
     1441 aagtgcgtcg tggataccaa ctcgtaaggc aactcccgtt ccaacgtcct ttgcgaggtg
     1501 tacaggccca ggttagctcc cggttcaagg acaccgataa ccgtcacctc aaaggtcgcc
     1561 tcgctgaagg agtcgcgtac ttcgatggtt attggttgca tgctttcgtc ttcgatatag
     1621 accccctcta gcttgaaatc aggcgcgcca atgacgatgc tggtcgtctg gcgggagggc
     1681 acaccgaaac tatcaatgac ggcatagccg cgctcgtcgc gcacggcgcg ccatacctca
     1741 gcgcccgact tgtatccttc tgccatcacc gtcaggtcaa aggccacgtt ctccaggtag
     1801 tcgtcatcta ctccgtagat caggtagtcc ctccactgcc cccgcccctg gcgcgcctct
     1861 atgcccaggc tggaacgatc cgcaacgacc aaaaagtccc tctggttaat atccctgttg
     1921 gaggcgatag ccgcctccag gtccggcacg gggttggtgc ggctagattc cgtgcgcagg
     1981 tcaaaaccgc cagtcaaggc atcggtgtct gcgaaaaagg tctcattaac cttaaatgca
     2041 gtggccatga agatgacact gaagaccacc acagcgaaca tgcccatggt caggccagtg
     2101 cggaagcggt tagccagggg gtaagcgatg gacatacgta aaaccggagt cagacgccgg
     2161 gctccgccgg tcagccgcag gatgatcttc aggagcagat cggtgttgta catcaccagc
     2221 agcacagccc cagccaccag caggatcccg gaaatgaaca gcatctccgg cccagatttc
     2281 aggtctttga cgcccagggg ttcaaagaaa ccagacggca gggaccaaaa gataaccagc
     2341 ccgagaccca gcaaggtgaa tgcgagccgg tccgccagcc cgggtcgctt acgcagcgca
     2401 agcgagagca agccccgtaa agtcaaacac acgcctatga tgaggaacga gccgcccaga
     2461 gaaaatgccg ccccactgga ttgtgccacg ccagcgggtg tcaggatcac acctacgagc
     2521 agcgtcaacg gcccaccgat gagacccgcc cacacgaccc ctagcaggct gagcggcgct
     2581 tgcacccaaa aggtccatag ggcgcgcccg atccgcaagc gccgcaggtg gcggaagccc
     2641 tccaccagga cgcggaaagg agcaaagagg cggtcccgca atcgggtggg tggtgagggc
     2701 ggttccggga ggtcacggac ggccgctaca atattcagct tgctgacttt gtaggccgaa
     2761 aagagaacag taccgaaggt caccagcatg ccaagtgagt aagagacgat catgctgcgg
     2821 atcgtcaagt taggccgaat ggcaagatca gattgggctg caaatgcctg acccaggctg
     2881 gcggcgagca cgtacccagt cacgattccc agccctactc caactgcagc tgcacccagg
     2941 tcgtaggctg tcccttcaaa cacgaacatc tccaccaggt gcatacgttg gccgccaatg
     3001 gcacgggtca tgcccatctc aggttttcgt tcggctgcta acatgacaaa gataagaaat
     3061 atcaacaaca gacctgctgc gatgccgaaa agaccggtca caatgaagat cgtggtgaag
     3121 gcgctggcgg ctaactcacc ctggtccagg ttgtcgcgtt tcacatcaac cacttccaac
     3181 tcggacagat ccacaaacag ttcagccagc cgatcgcgtg agggttgatt catccactcg
     3241 gcctggtcca agatgacctg cacctcgtcg gcaacctcct cgtcagccag caagctcagt
     3301 aactcattgg ataacacgcc ggtctccagg aaatcggtta aggctaataa atctagctgg
     3361 atgttcccgc gtaaatcgct ggcttccccg cgcaaagttc ccaatatcga cgcgtcacgc
     3421 aagagtaaat taacaatcct gcccgcaaca actcgatctg tcagcaagcc acgcaggtgc
     3481 gttgtgaccg gttggctgag gtttgctcca gtggaagcat ccccgatatt ggagatgaag
     3541 atctcgttga tcctacctcg ttgtttgaaa agcgtctgcg cacgccgcag gggtaaaaac
     3601 atgcgcgggc tcttaccacc ggcagtaaca ctgcgcacct ggacgatctt tggtttcgat
     3661 tcggtataca gctcaagttt atcgcccggt gaggcgttta ggtcttgagc tgcggccgcg
     3721 ttgaggtaca cttcgcgcgg tcccagttgt gttaggttga gaggttttcc ctccacgtcg
     3781 atcagctcct cgttttcgag gatggcagtg tcactttgac gcagaccgat cactgttaat
     3841 gaccgctcac ttttgcgccg cgtggtattg accaccggaa tcgactcgcg tacggcaggc
     3901 acgaagaaat cgatcatctc gaagtcttcc aatgccgtca ccaattcctc atagcgggca
     3961 aatttgaagt aggtctgctg tgtagttccg ctacctgcgc cactcaggcg tattatttca
     4021 tctacctcgc ctaactcgtc tagggttagt gagcgaatcg tgtgagacat cgtgtcgccc
     4081 atgaagaaag ccgcagtgat gatcagcgtg gccaacatca agccaaaaac gataagggca
     4141 gattgcgttc ggcgtcgcgc gatgggacgt atccccaatt tgaacatgac ccgctcacgc
     4201 aaggccagca aggccaagac aacgatcact agtgcaaggc ttgatcccag gcaaccggcg
     4261 atcatattca tttctaagcc gaagagcttt tccaccgcta actcgtttcg ttttcgatca
     4321 ggccatcgcg catgcgcacc atacgaccgc aacgcgcgcc tacctgacga tcgtgggtca
     4381 ccaagataaa ggtcaatcca gcttcgacgt ttagacgcaa cagcaagtcc atgatgtctg
     4441 tggcggtttc ggaatctaaa tcgccggtgg gttcatccgc ccatacaatt gctggacggt
     4501 tagccagagc tcgggcgatg gttgttcgtt ggcgctcgc
//
LOCUS       DBNHOH010000665         3734 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1707125, whole genome shotgun sequence.
ACCESSION   DBNHOH010000665 DBNHOH010000000
VERSION     DBNHOH010000665.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1707125"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="AC16V7_11545"
     CDS             <1..200
                     /locus_tag="AC16V7_11545"
                     /inference="COORDINATES: protein motif:HMM:NF025819.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HIR6257798.1"
                     /translation="QGWDLIGIYHSHPLGQDGPSQTDIDEAYYPEVVSVIVSGRDGDW
                     RCRGFHIWDGQTHEVPVLIED"
     gene            283..984
                     /locus_tag="AC16V7_11550"
     CDS             283..984
                     /locus_tag="AC16V7_11550"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR6257799.1"
                     /translation="MSFVLSVAVIICAYLIGSIPSGLLIVKLTKGVDIRQIESGRTGG
                     TNAMRAGGFWAGLGTAIFDMLKATVCVWLARGLIPGAIWLEIIAPVMAILGHNYSIFL
                     LERGEGGIQFHGGAGGAPAAGGALGLWLPSMFFIVPLAAIILYFIGYASVATLSIPLV
                     TIVIFAIRAWIGAAPWEYVLYGLIAEVLLLWALRPNLRRLFNGTERLVGYRAKRKRKN
                     TDYSTSSSSTPSGSS"
     gene            complement(942..1847)
                     /locus_tag="AC16V7_11555"
     CDS             complement(942..1847)
                     /locus_tag="AC16V7_11555"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6257800.1"
                     /translation="MIQRERVADNVYSFQSDLYAQVTAGAVIGPSWAIVIDTLALPEE
                     TLAIREYIEQELGVPIRYVINTHYHADHAWGNYFFPGATVIAHALCRKWLEERGAPSI
                     NEMHKQNLAFRNIKIILPHIIFDEGQLGLRVGKKTLSLMPMPGHCADNIAILVEEDRV
                     LFAGDACMPLPYIVDGDIDDLTTSLKKVARMGLENIVQGHGDIILRGEIDSYIKDNLS
                     YLSAIRKAVRKASRRKYPMDMLDEVSIEDCGKSRVLIGGLAVELHRRNLRALYRQIFG
                     VLPQSSMDDEEDYDDPDGVEDEEVE"
     gene            2409..>3734
                     /gene="valS"
                     /locus_tag="AC16V7_11560"
     CDS             2409..>3734
                     /gene="valS"
                     /locus_tag="AC16V7_11560"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIR6257801.1"
                     /translation="MPEFSLPKTYDFKAVEQRIYEWWDRMGYFKPSNDPKKPGFDSSK
                     KPYVISIPPPNVTGELHLGHSMFVSMEDLMIRYHRMKGISTLWVPGSDHAGIATQLQV
                     EKSLAKEGLTREQIGREEFLRRTWLWKDKFGGIITGQIRRLGASCDWERERFTLDEGL
                     SRAVREAFVRLYEKGLIYRGPRLINWSPGLKTAVSDLEVEYSEEPGTLYFFKYILEDN
                     EKEYLPVATTRPETILGDTAVAVHPQDSRYQRFIGRNVIVPILGRAIPVIADEYVDRE
                     FGGGALKVTPGHDPNDYAIAERHGLPIISVLDEAGRINENGGPYRGQDRFEGRKNLWD
                     DMRSAGLVIKEQPYTLNVPRTQRGGEIVEPMISTQWFVHIQPLADAALQAVRDGRIRI
                     VPERFTKVYYNWLENIQDWCISRQLWWGHRIPVWYCADCGEMTVTRIDPE"
BASE COUNT          912 a          845 c          993 g          984 t
ORIGIN      
        1 cacaggggtg ggatttgatt ggcatctatc actcccatcc attgggacag gatggaccct
       61 cgcaaaccga tattgatgaa gcttattacc ccgaggtggt ttccgtgatt gtgtcaggac
      121 gagacggaga ttggcgttgc cggggatttc atatttggga tggccaaacc cacgaagtgc
      181 cggtcttgat cgaagattaa tcttgttctg agagtaactc tgcgcaagaa attgtgttat
      241 cctttacgcc gaaatagtat tgcctaggag gagccgttta atatgtcgtt cgtattatcg
      301 gttgctgtga tcatttgcgc ctatttaatt ggatcaattc caagcggctt gctgattgtg
      361 aaattaacca agggtgttga tatacgccag attgaaagtg ggcgcactgg cggaacgaat
      421 gcgatgcggg caggcgggtt ttgggctggt ttggggacag ccatcttcga tatgcttaag
      481 gctacggtat gtgtgtggtt ggcgcgggga ctcataccgg gagcgatatg gcttgaaata
      541 attgcccctg taatggcgat cttgggacac aattattcaa tatttttgct agaacgtgga
      601 gagggtggta ttcaatttca tggaggtgct gggggcgcac ctgctgcagg tggagctttg
      661 ggtctttggc tccccagtat gttttttata gttcccttgg cagcgataat tctatatttt
      721 attggttatg cctccgtggc taccttgagt atacctttgg tgaccatcgt gatatttgcc
      781 attcgagcgt ggattggtgc agccccatgg gaatatgtac tgtatggctt gatagcagag
      841 gtgctattgc tttgggcgct acggcccaat ttgcggcgcc tttttaacgg taccgagagg
      901 ctggttgggt atcgggcaaa acggaagaga aaaaatacag attattctac ttcttcatct
      961 tcaaccccat cgggatcatc gtaatcttct tcatcatcca tagagctctg ggggagcacg
     1021 ccaaatattt ggcgataaag ggctcgcaag tttcgtcgat gcaactctac tgctaatccg
     1081 ccgatcagca ccctgctttt tccgcaatcc tcaatgctga cctcatctaa catgtccata
     1141 gggtactttc gccgagaggc tttgcgcacg gccttgcgga tggctgaaag ataggagaga
     1201 ttgtctttga tatagctgtc gatctcgccg cgcaggatga tgtctccatg gccctgaaca
     1261 atattttcta gccccattcg cgccactttt ttcaatgagg tggtcaaatc gtcgatatca
     1321 ccatctacaa tatatggtaa aggcatgcag gcatcgcctg caaagagtac ccgatcttct
     1381 tccaccaaaa tggcgatgtt atcagcgcag tggcctggca tcggcattaa gctcagcgtt
     1441 ttcttaccta ctcgtaagcc cagttgacct tcgtcaaaga tgatatgagg caagatgatt
     1501 ttaatattgc gaaaggctag attttgcttg tgcatttcgt taatggaggg agcaccgcgc
     1561 tcctctagcc acttacggca caatgcatgt gcaatcacgg tggcacccgg aaagaaataa
     1621 tttccccagg catggtcggc gtgatagtga gtatttatca catagcgaat aggaactcca
     1681 agttcctgct cgatatattc gcggattgct agcgtctcct cggggagagc cagcgtatcg
     1741 atcactatcg cccagctagg accaatcact gcacctgcag ttacctgggc gtaaaggtca
     1801 ctttgaaagg aataaacgtt gtcagctacg cgttctcttt gtatcatgat cagtcaccat
     1861 ttataagtgt gttggtgtag aatagcataa tcctggggtg taagtcaaga aaaattaatc
     1921 tttttccaat gttgaccaca tataccaagc cggttataat tttgccaaca tctgaatagc
     1981 catatccagg aggagtaagc aggcaagacc ttacaccaga gaagggaagg ccactggctg
     2041 gaagcccccg tgagtgtctc ctgccgaagg tcgcctggat taattgctga agaaatcgag
     2101 cgtaatgctt cgagagtaga acggcacggg aagcccgtta cagcgaagcc ctgatcggta
     2161 tcaaattact ccagtaaggc ggttgagatt ttgggagatc tgtcagggtg ataagagcgc
     2221 cagttaatgc ctggcgaata gaggtggtac cgcggaaggc gcgccctttc gtcctcgtgg
     2281 acgcaagggt gttttaattt gtgggctaca atatgtgtct agcaaaatat tttctatcac
     2341 aagtcttcac ccactcttca gattgcacaa ggttacaaat gattttttag cactttagag
     2401 gaactaagat gcccgaattc tctcttccaa agacttacga ttttaaggcc gtcgaacagc
     2461 gtatctacga gtggtgggac cggatggggt atttcaaacc ctcaaacgac cccaaaaagc
     2521 cgggttttga ctcgtccaaa aaaccctatg tcatttccat cccccctccg aatgtgaccg
     2581 gtgagcttca tttgggtcat tccatgttcg tctcgatgga agacctgatg atacggtacc
     2641 accgtatgaa aggtatttcc actctgtggg ttcccggtag cgaccatgcg ggtatagcta
     2701 cccagttgca ggtggagaaa tccctagcca aagaaggttt gacacgagag cagattggcc
     2761 gcgaagagtt tttgcgccgc acgtggttgt ggaaggataa atttggcgga ataatcaccg
     2821 gccagattcg ccggctaggt gcatcttgcg attgggagcg agagcgtttt accctcgacg
     2881 agggcctgtc ccgcgcagtt cgcgaagcct ttgtgcgatt atatgagaaa ggtttgattt
     2941 atcgcggtcc gcgacttatc aattggtctc ctggattaaa gaccgcagta tcggacctgg
     3001 aagtagagta ttcggaggaa cctggtacgt tgtatttctt taaatacata ctggaggata
     3061 atgagaaaga gtatctacca gtagccacca cccgtccaga gaccatcctt ggcgatacgg
     3121 ctgtagccgt ccatccgcag gattcccgtt atcagcggtt tattggacgg aatgtaatcg
     3181 tcccgattct aggtcgagct attccggtta ttgcagatga atatgtggac cgcgaattcg
     3241 gtggaggtgc gctaaaagtc acaccagggc acgatcccaa tgactatgcc atcgccgaac
     3301 gacatggctt accaataatc tccgtcctgg acgaagcagg tcgtattaac gagaacggtg
     3361 ggccctaccg ggggcaggac cggtttgaag gccgtaagaa tttatgggat gatatgcgct
     3421 cggctggatt ggtaattaag gagcaaccat acactttaaa tgtgccccgg acgcaacgtg
     3481 gcggcgaaat cgttgagcca atgatatcga cccaatggtt tgtgcatatc cagcctttgg
     3541 cagatgccgc cctccaagcg gtgcgcgatg ggcgcatccg gattgtgcca gaacgattca
     3601 cgaaagtata ttacaactgg ctagagaata tccaggattg gtgcattagc cgccagctat
     3661 ggtggggtca ccgtatcccg gtatggtatt gcgcagattg cggggagatg acagtaactc
     3721 gcattgaccc ggaa
//
LOCUS       DBNHOH010000666         2081 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_489180, whole genome shotgun sequence.
ACCESSION   DBNHOH010000666 DBNHOH010000000
VERSION     DBNHOH010000666.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_489180"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..831
                     /locus_tag="AC16V7_11565"
     CDS             103..831
                     /locus_tag="AC16V7_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HIR6257802.1"
                     /translation="MTQFFAKDYTGGAFELFGSAHLVALALVLFINLVIVASRKSFTD
                     LGKKRFRFGLAALLVINELAFHLWNYSIGQWTIQTMLPLHLCSVLVWVSAYMLLTRSY
                     SIYEFAYFLGIGGAIQALLTPDAGIYGFPHFRFFQTIISHGAIVTAAIYMTLVEGYRP
                     YWSSLVRVAIWGNSYMAAVGIVNWLVGSNYLFIARKPDTASLIDMLGPWPWYIISLEV
                     VAILMCLALYLPYALRDRRIKAAQ"
     gene            complement(906..1118)
                     /locus_tag="AC16V7_11570"
     CDS             complement(906..1118)
                     /locus_tag="AC16V7_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIR6257803.1"
                     /translation="MSTEKRLYRSRDNRMIGGVCAGLGEYLGVDPTVVRLLFVLATFF
                     GFGSLLLVYIAMLLIVPEEPIETPAV"
     gene            complement(1201..1872)
                     /locus_tag="AC16V7_11575"
     CDS             complement(1201..1872)
                     /locus_tag="AC16V7_11575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR6257804.1"
                     /translation="MTTYRPIPLPNYKVYPPEEMIQRATRFRGDMQRRRSVRQFSSRP
                     IPRKVIAECLLAAGTAPNGANLQPWHFVAVSDADIKRQIRQGAEKEEHEFYHGRAPKL
                     WLDALAPLGVDEHKPFLEIATYLIVIFAQSYGRLPDGRKIKNYYVTESVGIATGMLIT
                     ALHQAGLVTLTHTPSPMGFLNQILGRPSNERPYLILVTGYPAEDAQVPDITKKPLHEI
                     ATFFD"
BASE COUNT          451 a          526 c          574 g          530 t
ORIGIN      
        1 gaaagattgc cctggctctg accggccgct ggcctgagca gctaggtacc atcccggaaa
       61 ggcaatacga gcctccattt gaagccattc aggataattg acatgacaca attcttcgcc
      121 aaagactata ctggaggggc gtttgagctg tttggctccg cacacctggt tgctcttgcc
      181 ctggtgttgt tcatcaatct ggtcatcgtc gccagccgga agagttttac agatcttggc
      241 aagaagagat ttcggtttgg gcttgccgcg ctcctggtga tcaatgagct tgccttccac
      301 ctgtggaatt attccatcgg tcagtggacg atccagacga tgctgccatt gcacctgtgc
      361 agcgtactgg tctgggtgag cgcctatatg ctgctgacgc gcagttattc catatacgag
      421 ttcgcttatt tcttgggcat tggcggggcg atccaggcat tgctaacgcc cgatgcagga
      481 atatatggct ttcctcattt tcgattcttc cagacgatca tttcgcacgg cgctatcgtc
      541 acagccgcaa tctacatgac cctggtggaa ggctaccgcc catattggag ctccctggtg
      601 cgtgtggcga tctggggtaa tagctacatg gcggctgtcg gcatagttaa ttggctggtt
      661 ggcagcaact atcttttcat cgcccgcaag cccgatacag ccagcctgat cgatatgcta
      721 ggtccatggc cttggtacat catcagcctg gaggtggtcg ccattttgat gtgcctagcg
      781 ctctatttgc cctatgcact gcgcgaccgg cgcataaaag ctgcacaata atcgacgact
      841 ggcaggcaca catttgcctg ccagtcgcct tctgaatttg cttatcgaca ttgagtgagc
      901 ttgatttata cggctggggt ttctatcggc tcttccggta cgatgagcag catggctatg
      961 tagaccagca gcaaagaacc aaaaccgaag aatgtggcca atacgaacag caagcgtaca
     1021 acggtggggt caacgccaag gtattctcct agccctgcac acacaccgcc gatcatccgg
     1081 ttatctcttg agcggtatag acgtttttcg gtagacatat cagttactcc ttgaatagtt
     1141 ttgttgattc tgtttacgaa aaacgtcgca cttggttgca tgaaattact gcccaaattg
     1201 tcaatcaaaa aaagtggcaa tttcgtggag cggtttcttg gtgatatctg gaacctgggc
     1261 gtcttcggct gggtagcccg tcaccaggat caggtacgga cgctcattcg atgggcgccc
     1321 cagaatctgg tttaagaacc ccatcgggct gggtgtgtgg gtcagcgtca ccagtccagc
     1381 ctggtgtaga gcggtgatca gcatgccagt ggcgatgccg actgactcgg tcacataata
     1441 atttttgatt ttgcgaccgt caggcaatct cccatagctc tgcgcgaata tgacgatcag
     1501 atacgtagcg atctcgagga agggtttgtg ttcgtccacg cccaatgggg cgagcgcgtc
     1561 taaccagagc tttggggcgc gcccgtggta gaactcgtgc tcttctttct cagcgccttg
     1621 gcggatctgg cgcttaatat ctgcatcgct gactgcgaca aagtgccagg gctggaggtt
     1681 ggcaccattt ggagcggtcc cagcagccaa taagcactcc gcgatcacct tgcgagggat
     1741 gggacggctg gagaactggc gcacacttcg ccttcgctgc atatcgccgc gaaaccgggt
     1801 cgcgcgctgg atcatttcct caggaggata cactttgtag tttggaagag ggattggtcg
     1861 gtatgtggtc atggattgct cctgatggtg gataaaatcc gtggcttgtc gcgagaaaaa
     1921 acgcaagttt taatctgccg ggcgtatgct tgccaagaga tagtaattat cccggtaaac
     1981 ttgttccatc gcaggacatt gtgccgcaga gggattcaat ggcaaaacga catgggtgag
     2041 atgctcttct cgggctagct tcgccaatag gtcgcaatct g
//
LOCUS       DBNHOH010000667         3201 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1451432, whole genome shotgun sequence.
ACCESSION   DBNHOH010000667 DBNHOH010000000
VERSION     DBNHOH010000667.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1451432"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="AC16V7_11580"
     CDS             complement(<1..663)
                     /locus_tag="AC16V7_11580"
                     /inference="COORDINATES: protein motif:HMM:NF051685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7916 family protein"
                     /protein_id="HIR6257805.1"
                     /translation="MTSIKRLLDANASDFAAMTAPQLIESIRQAEGRTVAAEVIATAP
                     PLLDKVSNVELAAGMGADLILLNLYDVTAPQVFGIPMQDAPGGIEIFGRIPSGKGATI
                     GVVKSFVGRPIGLNLEPIENPEAVKGSGRLANADNARMAVEQGADFILVTGNPHTGVT
                     NRGIRRVLAEIRGAVGDRVVLLAGKMHAAGVSGPPVSDADISAFADEGADGIVLPAPA
                     TVP"
     gene            complement(678..1145)
                     /locus_tag="AC16V7_11585"
     CDS             complement(678..1145)
                     /locus_tag="AC16V7_11585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR6257806.1"
                     /translation="MRIVPVYSPFTGRVIPLEDVPDPVFSAKMLGDGLAVLPETDQVV
                     APISGKLTVLHSAGHAFAVQAHEAPVTVLVHIGLDTVKLKGVGFKILTSLGDQVTLGQ
                     PVVQVDREAIEHAGYSLLSPVILPDLPEGIKVELTDTAKVVQGVDVLLNVVVP"
     gene            complement(1142..1387)
                     /locus_tag="AC16V7_11590"
     CDS             complement(1142..1387)
                     /locus_tag="AC16V7_11590"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIR6257807.1"
                     /translation="MEEKARQFIEALGGKENIVEIEGCITRLRGTLRDPSKVDMKKLQ
                     SLGVIGRPILMGKGIQVVVGTHAELIGTAMNKIMRAS"
     gene            complement(1566..2660)
                     /locus_tag="AC16V7_11595"
     CDS             complement(1566..2660)
                     /locus_tag="AC16V7_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIR6257808.1"
                     /translation="MFATLQRIGRSLMLPIAVLPAAGILLRFGQADLLNIPWLADAGG
                     AIFGNLALLFAIGVAVGFTADVGTAGIAGAVGYWVFTKVLVDISNSIYQAEVNMGVLA
                     GILMGLTAAWLYERYHTIQLPEWLAFFGGKRFVPIVTAFVALVLGIVFGFIWHWPEQW
                     LAAVGNWATGAGPMGAAVHAFLNRLLIPLGLHHVINSIVWFQFGDLNNFFDTAGQTGG
                     LFMTGFFPIMMFGLPAAALAMYVTAKDANRRAIGGVLFSAALTSLITGITEPVEFAFM
                     FVAPVLYVVHALLTGVSAFVTIALGLRDGFTFSAGLIDYILNFGIATNPIMLLVVGVI
                     FGVIYYFVFVTLIKALNIPTPGREPEVVEE"
     gene            complement(2791..>3201)
                     /locus_tag="AC16V7_11600"
     CDS             complement(2791..>3201)
                     /locus_tag="AC16V7_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257809.1"
                     /translation="YVLPKQVKILRSEKARQAGSSGNLTDVLSLIERSGVSLKSATEA
                     TIVIGAVAQIPPPTSDTATLIFIDDTSRGEYTGYSRIVRLPGRNIPISQMLNDVPYAL
                     KVINLRAELGAGYLTAKLGSNLTRIVRRMLGGKV"
BASE COUNT          810 a          917 c          792 g          682 t
ORIGIN      
        1 tggcacagta gccggagcag gcaagacgat tccatccgct ccttcgtcgg cgaaggcgct
       61 gatatccgca tcacttacgg gtggcccaga cacccccgca gcatgcattt tccctgccag
      121 gagcacaacc cgatcaccca ccgccccgcg gatttctgcc aggacgcggc gaatgccgcg
      181 gtttgtcacg ccggtgtggg ggttgccggt gaccaagatg aagtctgcac cttgctccac
      241 ggccatacgg gcgttgtccg cgttagctaa ccgcccggag ccctttacag cctctgggtt
      301 ctcgatgggt tccaggttca agcctattgg gcgtccaaca aaagatttga ccacacctat
      361 tgtagcgccc ttacccgagg gaatgcgacc aaaaatttcg atccccccag gggcatcctg
      421 catgggaata ccgaaaacct gcggtgcggt tacatcgtac aagtttagca agatcaagtc
      481 ggcccccata cccgctgcca actcgacgtt agagacttta tccagcaacg gcggggcggt
      541 ggcaatcacc tcagcagcca ctgtgcgccc ctctgcctgg cgaatgctct caataagctg
      601 aggcgcagtc attgcggcaa aatctgacgc attggcgtct aataagcgtt taattgacgt
      661 catcacgatt tggatcctta tggaacaact acattgagca gcacatccac cccctggacc
      721 acttttgcag tatccgtaag ctcgaccttt atcccctcgg gtaaatctgg caatattact
      781 ggtgaaagaa gagaatatcc agcatgttcg atcgcctctc gatcaacctg aaccactggc
      841 tgtccaagcg tgacctgatc gccaagactg gtcaagatct tgaacccgac gcccttcaac
      901 ttcacggtgt ccagcccaat atggactaaa acagttaccg gggcttcatg cgcctggaca
      961 gcaaatgcgt gcccggcgct atgcagcaca gtcagctttc cactgatcgg cgccaccacc
     1021 tgatctgtct caggcaggac cgccaatcca tcaccaagca ttttagccga gaataccggg
     1081 tctggaacat cctctagagg tattaccctg ccggtgaaag gggaatagac cggaacgatc
     1141 ctcatgaagc ccgcatgatt ttgttcatgg ctgtgccgat taactcagcg tgtgttccga
     1201 ccaccacctg aatacctttg cccatcagga tcggccgacc aatcacaccc agcgattgca
     1261 gtttcttcat gtccactttt gaagggtctc tcaacgtacc gcgcaagcgg gtgatgcaac
     1321 cttcaatctc gacaatgttc tctttgcctc ccagggcttc aataaactgc cgggcctttt
     1381 cttccataat ctaaataacc tcctcaattg ccaacaaagg ggaaattggg ctgtccaaaa
     1441 agaaaaaaga tccatcataa actctagctg taggccggat aatcactccg gctgcaacag
     1501 gcggcggttt atcaaaccgc acattttgga cagcccattt caaagggtac aaacttcttg
     1561 cagaattact cctcgacgac ttcaggctcg cgacctggcg tggggatgtt caaggctttg
     1621 atcaacgtca cgaagacgaa gtaatagatc acgccaaaga tcacgccaac taccagcagc
     1681 ataatggggt tggtagcgat gccaaagttg aggatgtagt cgatcaagcc agccgaaaaa
     1741 gtaaagccgt ctcgcaggcc aagtgcaatg gtcacaaatg ccgagacgcc ggtaagcagg
     1801 gcgtgcacga cgtacaacac cggagcgacg aacatgaaag caaattcaac cggctcggtg
     1861 atgcccgtta tcagcgaggt caaagcggcg ctgaagagca cacccccgat agctcgtcta
     1921 tttgcatctt tggcagtgac atacatcgcc agggctgctg ccggcagtcc gaacatcatg
     1981 atggggaaga agcccgtcat gaacaggcca ccggtttggc cagcggtatc aaagaagttg
     2041 ttcagatcgc caaactggaa ccagacgatg gagttgatca catggtgcag tccgagtggg
     2101 atcaacaagc ggttcaagaa ggcatgcacc gctgcgccca tggggcctgc gccagtagcc
     2161 caattcccta ccgccgccag ccattgctca ggccagtgcc agatgaagcc aaatacgatc
     2221 cccaatacca atgccacaaa ggctgtcact attgggacaa agcgcttgcc accaaagaaa
     2281 gccagccatt cgggcagctg gatggtatgg taacgctcat ataaccaagc agcggtcaat
     2341 cccattagaa tgccagccag aactcccatg ttcacctcag cctggtagat actattcgat
     2401 atatcaacca ggactttggt aaacacccag taacccacag cgccggctat accagctgtg
     2461 ccgacatcag cagtgaagcc tacagccacg ccaatggcaa atagaagtgc caggttccca
     2521 aagatcgcgc cacccgcatc tgctagccag ggtatgttaa gcaggtcagc ctgtccaaag
     2581 cggagcagga tacccgccgc aggcagaacg gcgatcggga gcatgagcga acgtccaata
     2641 cgttgtagtg tagcaaacat tgaatcctcc taactaaatg ataatctgga tagaattatg
     2701 atgagaggct ctggtgaaaa tttgtcaccc gtactcgagc ccatcgaatt ttttgttcgc
     2761 gattcacctc ctcattgtgc gagttgatta tcagactttc ccacccaaca ttcgtcgcac
     2821 gatacgtgtg agattggagc ccaattttgc cgtcagatag cctgcaccta attcagcgcg
     2881 caggttgatc actttcagag cataagggac atcgttcaac atctggctaa tcgggatatt
     2941 tcgccccggt aatcgaacta tgcggctgta gccggtgtac tcgccgcgcg aggtatcgtc
     3001 tatgaagatc agtgtagccg tatctgatgt aggcggcggt atttgcgcca ctgcaccgat
     3061 gacgatggta gcctccgtcg ctgatttcaa gcttacaccg gagcgctcta tcaaggacaa
     3121 cacatcggtc aggttgcctg aactacccgc ctggcgggct ttttctgagc gcagaatctt
     3181 cacctgcttg ggtaatacgt a
//
LOCUS       DBNHOH010000668         5559 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_104229, whole genome shotgun sequence.
ACCESSION   DBNHOH010000668 DBNHOH010000000
VERSION     DBNHOH010000668.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5559)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_104229"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="AC16V7_11605"
     CDS             <1..1076
                     /locus_tag="AC16V7_11605"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR6257810.1"
                     /translation="FDLWRDQFPVLNQAFDILGDNGLKPMPEKPALEYGMIRGMLQAY
                     DDPYTLFVEPPQHELESNALQGSFGGIGVRLTRDLEGYVILYPFQGSPASEMGIMDGD
                     RLIFVDDMEISPEVTIESVQAALRGPVGQSVRVTISRPPDNGQLEFIIRRAEIPLPSV
                     AWHIDSDIPEVGVIELNIIAASTVQEVQSAIRDLQQRGAQYFILDLRDNFGGLLTAGV
                     DVARLFLDNGVIMEYENRDKKIEVYRVEQPGEFVDVPLVVIVNENTASASEIIAGALK
                     AHQRAQIVGTHTYGKNTIQLVFDLMDGSSLHVTSAQWWIPGLEQPIGKGGLEPDIYTE
                     TTPDEAGVDAAIREASLVLMRLK"
     gene            1169..2011
                     /locus_tag="AC16V7_11610"
     CDS             1169..2011
                     /locus_tag="AC16V7_11610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR6257811.1"
                     /translation="MKAKKRRVGCGCGSFFGGLLLALVIILTIYLFAPLRTNLLLLGI
                     DYAPPGSSVSRSDTIILATVIPTEPYIGVLSVPRDLWVNIPGIGENRVNTAHFFAEAQ
                     QVGSGPKAAIDTIQQNFGVDVDYFVRIRFDGVRSVVDAMGGVDIDLDRPMAGYPAGRH
                     HLTGNKALAFARHRLGADDFFRMEQGQLLIKAIFRQLLHPSMWPRLPSVVVAIMNEVD
                     TNIPLWQWPRLALALLRAGPDGLDNRTLERDMTTPFTTNQGASVLLPDWSKINPLIED
                     MFGY"
     gene            2085..2540
                     /gene="smpB"
                     /locus_tag="AC16V7_11615"
     CDS             2085..2540
                     /gene="smpB"
                     /locus_tag="AC16V7_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIR6257812.1"
                     /translation="MGIKIVAKNRKASHEYFLLDTYEAGLVLQGSEIKSIRAGQISLA
                     EAYVQVDGSEAWLMDAHIAPYAQASIYNHEPRRPRKLLLHKVEIRRLWNEVRKKGVTI
                     VPTKVYLKEGRAKVEIALAKGKKLYDKRDAIAKRDLQREMERKGRRYEG"
     gene            2540..3340
                     /locus_tag="AC16V7_11620"
     CDS             2540..3340
                     /locus_tag="AC16V7_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257813.1"
                     /translation="MGPNKQQVYTISSINRLPSEEKKEIYTRLIPSEMLQLFQIDLKS
                     LYREGANLLKLKAPSGSSVVEMELRHQLDFPDPILYGHMMDTLNGHIHVLLYILNDPN
                     SPRFDVDRLADGQPTKFGTRARNIDAEQAAMEYGLAPGQVRRGLRLLGSAIQAFERFI
                     ESLGQEMYFTEPLYYHNAVLFEQHGFAYEKGRKLMERIQKGFMQGGDLVPLLDNSTPF
                     RSPLAANSIRLRSWAIHDNLLGETFTDVTMYKRVGKTASINTCLDCTW"
     gene            complement(3348..>5559)
                     /locus_tag="AC16V7_11625"
     CDS             complement(3348..>5559)
                     /locus_tag="AC16V7_11625"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR6257814.1"
                     /translation="LTSGGDAQGMNAAVRAIVRSGLDIGLEVYAIYEGYRGLVEGGQY
                     IRKMTWDSVGGILQRGGTIIGTARSDEFRTRPGRLKAAQNLIENEIDNLIVIGGDGSL
                     TGGNIFRQEWPGLLADLVADGQLQQAQADRHPNLLIAGLVGSIDNDFFGTDMTIGADS
                     ALHRITEAIDAITSTASSHQRTFVVKVMGRNCGYLALMGALATGADWVLIPESPPAVD
                     NWEDLMCERLKAGRKAGRRDTIVVLAEGARDRYGNYIGSSLVAEILEKCMGEEVRVTV
                     LGHVQRGGAPSAFDRNLGTLLGHAAVDLILSSSPDTEPQVVGLRGNRITHTPLVECVE
                     KTQAVAKAIENHAYDQAMELRGSSFRETFSTLKTMLRALPHPPQPDQRRLRLGVIHSG
                     MPAPGMNTAVRAAVRIGLDRGHIMLGMQNGFQGLIDGDIQELDWMSVNGWASLGGSEL
                     GTNRKVPSGSDLYHIARNIETHQIQGLLIIGGWAGYESAYHMLQERKNYPAFEIPMVL
                     LPASINNNLPGSDLSVGADTALNNIVEAVDKIKHSAVALRRCFVVEVMGRKCGYLALM
                     SGLATGAERVYIHEEGVTLRDLMTDVDHLNKGFMAGKRLGLMIRNEHANDVYNTAFMC
                     ALFEEEGGGLYDVRQSILGHLQQGGNPSPFDRIQGTRLATRCIDYLINQANQGSTSCA
                     FIGLQGREIIFHDLQDFPRVGDLENQRPKTQWWMELRHIAKVLAQPAPHSMIDQ"
BASE COUNT         1395 a         1312 c         1409 g         1443 t
ORIGIN      
        1 attttgatct atggagggat caattcccgg tcttgaatca agccttcgat atcctgggtg
       61 ataatggatt aaaaccaatg ccggaaaaac ctgccttaga gtatggcatg atccgtggga
      121 tgttgcaggc atatgatgat ccatatacct tgtttgttga gccgcctcag catgaactgg
      181 aaagtaacgc attgcagggt agctttggag gtattggtgt tagattgact cgcgatctgg
      241 aaggatatgt cattttatat ccattccagg gaagtcctgc cagcgagatg ggtattatgg
      301 atggtgatcg gcttatcttc gtagatgata tggagatatc gccggaagtt acaattgaaa
      361 gcgtccaggc ggcgctcagg ggaccagttg gacaaagcgt gcgtgtaacc atatcacgcc
      421 cgcccgataa tgggcagctc gaatttatca tccgacgcgc ggaaattcca ttgccatcgg
      481 ttgcctggca tatagactct gatattccag aagttggggt gatcgagcta aacatcatcg
      541 ctgccagtac tgtgcaggag gttcaatccg cgatacgcga tttgcaacag cgaggtgcgc
      601 aatatttcat cctcgacctg cgagacaatt ttgggggttt gcttacagca ggagtggatg
      661 tcgccagact gttcttggat aatggggtca ttatggaata cgagaatcgc gataaaaaga
      721 ttgaggttta tcgcgtggag cagccaggag aatttgttga tgtgccgctt gtggtgattg
      781 tcaatgagaa taccgctagc gcttcggaaa taattgctgg ggcgttgaaa gcgcatcagc
      841 gtgcccagat tgttggcacg cacacatatg gaaagaatac aatccaatta gtgttcgacc
      901 tgatggatgg ctccagcctg catgtaacat cagcgcagtg gtggataccg ggccttgaac
      961 agcctattgg aaaaggtggt ctggagccgg atatctatac agaaaccact ccagatgaag
     1021 ctggagtgga tgcagctatt cgggaggcat ccttggtctt aatgaggtta aagtaatgtc
     1081 acacgaaaat tgggtcgacc cccaatctca gccaaatgca tattatcctc ccccgatgcc
     1141 cccatcctac tcaagcccgc caaaatggat gaaagcgaaa aaaagacggg ttgggtgcgg
     1201 atgtgggtct tttttcggag ggttgctgct tgcgttggtc ataatactaa caatatattt
     1261 gtttgcaccc ctgcgaacga atttattgct tttaggtatt gattacgctc cccctggtag
     1321 ctcagtcagc cggagcgaca caattatctt ggcgacagtt atcccaacag aaccatatat
     1381 tggcgtgttg tcggtcccac gagatttgtg ggtaaatatt ccaggcatag gtgaaaatcg
     1441 cgtcaacacc gcccacttct tcgcggaggc gcagcaggta ggcagtggac caaaagctgc
     1501 cattgatacc atacagcaga atttcggtgt tgatgtagat tactttgtgc gcatccgttt
     1561 tgatggtgtc cgcagtgtgg ttgatgcaat gggaggtgtg gatattgatc ttgatcgccc
     1621 aatggctggc tatcctgcag gtcgccatca tctgacaggc aataaggccc ttgcttttgc
     1681 gcggcatcgg cttggcgcgg acgatttctt ccgcatggag caaggtcaac tgttgatcaa
     1741 agcgatattt cggcaactgc tgcacccttc tatgtggcct aggttgccca gcgtagttgt
     1801 ggcgatcatg aatgaagttg acacgaatat cccattgtgg cagtggcctc gtcttgccct
     1861 agcgttattg cgcgcaggac cggatggctt ggataaccga accttagaac gtgatatgac
     1921 gacacctttt acgacaaatc agggggcgag tgttctgctg ccagattggt caaaaatcaa
     1981 tccactaatc gaagatatgt ttggttattg atttagacat tgtggagctg gagaacatct
     2041 cccaaactac ttatatcatg tgattatgtt aaaatttgat tgcaatggga ataaaaatag
     2101 ttgccaaaaa tcgaaaagcc agccatgagt attttttatt ggatacgtac gaggctggtt
     2161 tggtgctgca agggagtgaa attaaatcta tacgcgccgg tcaaattagc ctggcggaag
     2221 cctatgtcca ggtcgatgga agtgaagcat ggttgatgga cgctcatatt gcgccctatg
     2281 cccaggctag catctacaac cacgaaccac ggcgccctcg aaaattactt ttacacaagg
     2341 ttgaaatccg gcgactgtgg aatgaggtgc ggaaaaaggg tgtgacgatt gttccaacga
     2401 aggtttattt gaaagaaggg cgtgccaaag tggaaatcgc tctggctaaa gggaaaaagc
     2461 tctatgataa gcgggatgcg attgccaagc gtgatctgca gcgcgagatg gagcggaagg
     2521 ggaggaggta cgaggggtaa tgggaccaaa taagcaacaa gtttacacta ttagcagtat
     2581 caatcgtttg ccctctgaag aaaaaaagga aatatatacc cggcttattc catctgaaat
     2641 gctgcagtta tttcagatcg atttgaaaag cttatatcgg gaaggcgcca atttgttaaa
     2701 gctaaaggct ccatctggga gttctgtagt ggagatggag ttgcgtcatc agcttgattt
     2761 tcctgatcct atcttatatg gacacatgat ggacacccta aatgggcata tccatgtttt
     2821 actttatatt ttgaacgacc ctaattcgcc gcgcttcgat gttgatcgtc ttgctgatgg
     2881 acagcccaca aaatttggaa ctcgggctag gaatattgat gccgagcaag ccgctatgga
     2941 gtatggatta gcccctggtc aagttcgcag ggggttacgt ttgttaggct cggctatcca
     3001 agccttcgag cgttttattg aatcgcttgg acaggagatg tactttaccg agccgcttta
     3061 ttaccataat gccgtccttt tcgagcagca tggttttgca tacgaaaaag ggcgcaagct
     3121 tatggagcgc atccaaaagg gatttatgca gggtggagac ttagtgccgt tgttagataa
     3181 ttcgacaccc tttagaagcc cactagctgc taatagcatc cgtttgcgct catgggcaat
     3241 tcacgataat ttgttggggg aaacctttac agacgtgaca atgtataagc gtgtggggaa
     3301 aaccgcaagt atcaatacct gtttagattg tacttggtaa agcgcaatca ttgatcaatc
     3361 atgctgtggg gcgctggttg ggctaatacc tttgcaatat gtcgcaattc catccaccac
     3421 tgtgtttttg gacgctggtt ctccagatct cccacgcgcg ggaaatcttg caaatcatgg
     3481 aatatgattt ccctgccttg caaaccgata aaagcgcaag atgttgaccc ctgatttgct
     3541 tggtttatga gatagtcgat acagcgcgtg gctaaacgcg tgccttgaat tcgatcaaag
     3601 ggcgaaggat tacccccttg ttgcagatga cccagaatag attgtcgaac atcatacaaa
     3661 cctccacctt cctcttcgaa aagtgcgcac atgaaggcgg tgttatacac atcattggca
     3721 tgctcattac ggatcatcaa acccaggcgc ttaccagcca taaagccttt gtttaggtga
     3781 tctacatctg tcatcaagtc gcgcagggtg acaccctctt catggatata tacgcgctct
     3841 gcaccagtag ctagaccact catcagggca agataaccac attttcgccc catcacttca
     3901 acaacaaagc acctgcgaag ggcgactgca gagtgcttga ttttatccac agcttcgaca
     3961 atattgttga gcgcagtgtc agcacctacg cttaaatcag aacctggcag gttgttgttg
     4021 atggaggctg gcagcaaaac catgggtatt tcaaatgccg gataattttt gcgttcttgt
     4081 aacatgtggt aagcagattc gtatcctgcc caacccccaa tgattaacag cccttggatc
     4141 tggtgtgttt cgatgttgcg ggcgatatga taaagatcgc tgccgctggg tactttccga
     4201 ttcgtgccaa gctcagaacc acccagggaa gcccagccgt tgacactcat ccaatccaat
     4261 tcctggatgt cgccatcaat taagccttgg aaaccgtttt gcataccgag catgatatgt
     4321 cccctatcca gtccaatgcg caccgcagcc cttacagcgg tattcatccc tggtgcgggc
     4381 attccagagt ggataactcc cagccgcaag cgtctttggt caggctgcgg agggtgaggc
     4441 aatgcacgca gcatggtttt gagtgtagaa aatgtctccc taaagctgct accgcgcaat
     4501 tccatggcct gatcatatgc gtgattttca atggctttgg cgactgcctg ggtcttttca
     4561 acgcattcca caaggggcgt atgggtgatg cggttgccac gcaatccgac aacctgtggc
     4621 tcagtatcag gcgatgagga caagattaga tcgactgctg cgtgtcccag gagagttccc
     4681 agattgcggt cgaaggcgct cggtgctccc cctcgctgca catgtcccag cacggtcacg
     4741 cggacttctt ctcccatgca tttctctaat atctcggcta ccaggctact tccgatgtaa
     4801 ttaccatacc tatccctggc gccttccgcc aatacaacga ttgtgtcgcg gcgcccagct
     4861 tttctgcccg cctttagccg ctcacacatg agatcttccc aattatctac cgctggtggg
     4921 ctttcaggga tgagtaccca gtcagctcca gtagccaatg cacccatcaa tgccaggtag
     4981 ccacaattgc gccccatgac tttcaccaca aaagtgcgct ggtggctgga ggcagtgctg
     5041 gtgatggcgt caattgcctc ggtgatacga tgcagggcac tatcagcccc gatagtcata
     5101 tcggtaccaa aaaaatcgtt atcaattgat ccaactagac cagctatcag caggtttggg
     5161 tgccgatcag cttgtgcctg ttgcagttgc ccatcagcta cgagatctgc aagcaaacca
     5221 ggccattctt ggcggaagat gttgccgccg gttagactgc catccccgcc aatgacgatc
     5281 aggttgtcaa tttcattttc aatgagattt tgcgcggctt tcagtctacc aggacgcgtt
     5341 cgaaattcat ccgatcgagc cgtgccgatg attgttccgc ctcgctgcag gataccaccc
     5401 accgaatccc atgtcatttt acggatatat tgaccgccct caaccaatcc cctgtaaccc
     5461 tcataaatag catagacttc taaaccgatg tctaaccctg aacgcacaat tgcacgaacg
     5521 gctgcgttca tgccttgtgc atctcctccg ctggttaga
//
LOCUS       DBNHOH010000669         4901 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1129660, whole genome shotgun sequence.
ACCESSION   DBNHOH010000669 DBNHOH010000000
VERSION     DBNHOH010000669.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4901)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1129660"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..2607
                     /gene="mutS"
                     /locus_tag="AC16V7_11630"
     CDS             22..2607
                     /gene="mutS"
                     /locus_tag="AC16V7_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIR6257815.1"
                     /translation="MSPKDDISPIRKQYLEIKRQYPNAILFFRLGDFYETFDQDAEVA
                     SRELDIVLTSRNVAKGTRVPMAGIPHHAAENYLARLIERGYHVAICEQVGDQPIKGLF
                     PRQVMRVVTPGTVIEPGLLPGDANNYLACVVISEDEDVRRAGVAYADITTGEFATTEL
                     QEGDLQTSVRAELIRLMPAEILHPDSYKTGNGLPGHLTAWPAWRFELGRCQEALLKHF
                     ETATLDGFGLRGLPLAVRAAGAILQYLGETQAASLKQLSRLSVYSLLDFMTLDAATRR
                     NLELTETIRGSSSKGSLLGVLDHTITPMGKRLIRQWVSKPLLDVEKICQRQDQVQFFY
                     TESMTRTELRSALKPLGDLERLTNRVVSGHAQPRDLVAMRNTLRQLPGLRGMFPSTDS
                     QMDPVLRDLHTCLDELGLLEAAIEDDPPATLQNIGVIRSGYSAELDGVVERSRHARQW
                     IAGLEGVERQRTGIKSLKVGYNKVFGYYIEVTKANVDMVPAEYIRKQTLVNAERYITP
                     ELKEYETLVLNAEERIREIEGRLFHEVCAQLGGSATKLLAVAGALAQLDVLAALAEAA
                     ILGNYVRPDVVVDDVLEINDGRHPVVEQMLVGERFVPNDVMFEASERVRIITGPNMSG
                     KSTLIRQVALIVLMAQMGSFIPADSARIGLVDRIFTRIGAQDEIHAGQSTFMVEMVET
                     ANILHHATPRSLLILDEIGRGTSTYDGVSIAWAVVEYIHNHPNLRAKTLFATHYHELT
                     QLADLLPGVRNYNVAVSEAEGKVVFLHKIIPGGADRSYGIHVGQLAGLPKPVIQRANE
                     IMRSLEASSGKAVRINPIAAKQMALFPETNPLLDELEDLDVNGLSPIEALNKLYEWQQ
                     KYLNK"
     gene            2612..3277
                     /locus_tag="AC16V7_11635"
     CDS             2612..3277
                     /locus_tag="AC16V7_11635"
                     /inference="COORDINATES: protein motif:HMM:NF024067.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HIR6257816.1"
                     /translation="MRESRKPNRPLSPQSNDRTMLLMARVDEMRVELRAQDPFKLMQR
                     VSGRYVQTQSGKGELRLALWGREIFVTFPDFIAFEGSSQQKLHAGLQALVIYYLYTTD
                     GAPIADRFISFSELPDGKFYAQAFQGYTGQELQHHFGDDRQRFERAAQNIGGRHYPLG
                     DASFTFQLLPRVVLLVVFWCGDEDFPSSYQVLFDASISHHLPTDVCAVAGSMLTRQLI
                     ESE"
     gene            3464..4099
                     /locus_tag="AC16V7_11640"
     CDS             3464..4099
                     /locus_tag="AC16V7_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257817.1"
                     /translation="MAENLQVTATAAARQLLLDEVGQWRLLLSDSYDEDLNDWSTGSD
                     EDPELGSISWVIEDGKYRWQGLATNGFVWWVIPDIESVSDFYLSVSARQEAQPEVGEY
                     GLVFRQSGDVDYYLFEISDLGLYSLYLHTDSGWESLIDWTAHPSINPGATNRLAVIAT
                     GEHFDLFINDTFVAEYSDDRLPEGKAGLLVGLSNPGDAANWEFDDFEFRAP"
     gene            4246..>4901
                     /locus_tag="AC16V7_11645"
     CDS             4246..>4901
                     /locus_tag="AC16V7_11645"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF019121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR6257818.1"
                     /translation="MKKFRVALLANLKVNAPKFEGMPEDQWDDLDSEKTINALVNAIR
                     AGGNSCEFLEGNLSLVDSLQKFKPDICFNICEGHFGDAREAHVPALLEMLRIPYTGSR
                     VLTLALALDKPMTKRVLAYHDLPTPPFQTFERVDEPLSTDMNYPLFVKPSREGTGMGV
                     SGRSIVRDEDELREQVDFILKRYKQPALVEGYIEGREVTLGVVGNLIGPVARRLPDDD
                     "
BASE COUNT         1164 a         1179 c         1340 g         1218 t
ORIGIN      
        1 cacacaactt aaaatcaaga catgtctccg aaagatgata tcagcccgat ccggaagcaa
       61 tacctggaga ttaagcgcca gtatccaaac gctatcctgt ttttccggct gggtgatttt
      121 tacgaaacct tcgaccagga tgcagaggtg gcctcgcgcg agctggatat cgtgcttacc
      181 tcgcgcaacg ttgctaaagg tactcgcgtg cccatggcag gcatccccca tcacgccgca
      241 gaaaactacc tggcacgcct gattgagcgc ggctaccatg tggcgatatg tgagcaagtt
      301 ggagatcaac ccatcaaggg cttgttccct cgccaggtta tgcgcgtggt aacaccgggc
      361 acggtcatcg agccaggctt gcttccagga gatgcgaaca attatctggc ttgtgttgtg
      421 atttcagagg atgaagacgt aaggcgcgct ggagtagcct atgcagatat cacaacaggt
      481 gagtttgcca ccactgagct tcaagagggt gacttacaaa cgtctgtgcg tgctgagcta
      541 attcgtctta tgccagctga gattctgcat ccagatagct ataagacggg gaacggtttg
      601 ccagggcatc tcactgcttg gcctgcctgg cgtttcgaat tgggtcgctg ccaagaagct
      661 ttactcaagc attttgagac cgctacattg gatggatttg gcttgcgcgg cttgccactt
      721 gccgtacgcg ctgccggggc aatcttgcaa tatctcggtg aaacacaagc tgcttctctc
      781 aagcaattat cccgccttag tgtctacagc ctcttggatt tcatgactct ggatgctgcc
      841 acgcgccgta acttggagct aactgagacg attcggggta gctcaagcaa aggctccctg
      901 ctgggtgtgc tggatcacac gatcaccccg atgggcaaaa gattgatccg ccagtgggtg
      961 agcaaaccct tgttggatgt tgaaaagatt tgccaaagac aagaccaggt gcaattcttt
     1021 tataccgaga gcatgacccg caccgaattg agatctgctc tcaagccttt gggtgatctc
     1081 gagcgcttaa ccaatcgtgt ggtcagcggg catgcccaac cgcgagattt ggtggcgatg
     1141 aggaatacat tgcgccagtt acccggattg cgtggaatgt tcccatcgac cgatagccaa
     1201 atggatcctg ttctaaggga tttgcacaca tgtttagacg agttggggct attggaagcc
     1261 gctatcgaag atgacccgcc tgctacacta cagaatattg gtgtgatcag gtctggctat
     1321 tctgccgaac tggatggtgt agtagagcgc tctcgacacg ctcgccagtg gatagcggga
     1381 ttggaggggg tggagcggca acgcacggga atcaagtcgc ttaaggtggg ctataacaag
     1441 gtctttggtt attacattga agtgaccaaa gccaatgtcg atatggtacc ggcggaatat
     1501 atccgcaaac agacgctggt caacgctgaa cgctatatta ccccagagct caaggagtac
     1561 gaaaccctgg tgctgaatgc cgaggagcgc attcgcgaga ttgagggtcg tctatttcac
     1621 gaggtatgcg cccaattggg gggctcagcg actaagctgt tagccgttgc aggcgctttg
     1681 gcgcagctcg atgtgctggc ggcgctggca gaagcggcta tcctgggaaa ttacgtgcga
     1741 ccagatgttg tggttgacga tgtgctggaa atcaatgatg ggcgccaccc ggtcgttgag
     1801 caaatgctgg taggagagcg ctttgtgccc aacgatgtga tgtttgaggc ttccgagcgc
     1861 gtgcgcatca tcactggacc taatatgtcg ggtaaatcca cgctgatccg ccaggttgcg
     1921 ctcatagtgc tcatggcgca aatgggtagc ttcatcccag ccgactcggc gcgtatcggc
     1981 ttagtggatc gtatcttcac ccgaatcggc gcacaggatg agatccatgc cgggcagtct
     2041 acatttatgg ttgagatggt cgagacggcc aatatcctgc accatgccac cccgcgcagc
     2101 ctgctgattt tggacgagat cgggcgtggt accagcactt acgacggggt ttccatcgct
     2161 tgggcagtgg ttgagtatat tcacaatcac ccaaacctgc gcgccaagac tctctttgcc
     2221 acccactatc acgagttaac ccaactggca gatttactgc ctggggttcg caactataac
     2281 gtagctgtga gtgaggcaga gggcaaggta gtctttctgc acaaaatcat ccctggaggt
     2341 gcggaccgct cttacggaat ccacgttggc caattagcag gcttacccaa gccggtcatc
     2401 caacgtgcca acgagatcat gcgttcctta gaggcttcgt cgggcaaggc ggtgcggatc
     2461 aaccccattg ccgccaagca aatggctctc ttccctgaaa ccaacccgct gttggatgag
     2521 ctcgaagatc tggatgtcaa tgggctctct ccgattgaag ctttgaataa gctttatgag
     2581 tggcaacaga agtatctgaa taaatgactt catgagagaa tcacggaagc ctaaccgccc
     2641 gctctcccct caatctaacg atcgcacgat gttactgatg gcgcgggtag acgagatgcg
     2701 agttgaattg cgcgcacaag atcctttcaa gttgatgcaa cgtgtttctg ggaggtatgt
     2761 gcaaacccaa tccggtaaag gggaattgcg cttggctttg tgggggcgcg aaatttttgt
     2821 cacatttccc gactttatcg ccttcgaagg cagttcacag cagaaattgc acgcgggatt
     2881 acaggcactg gtaatctatt acttgtacac tacagatggc gcacccatcg ctgaccgatt
     2941 tatctctttt tccgagctac ctgatggcaa attttatgcc caggcttttc agggatacac
     3001 cggtcaagaa ctgcaacatc attttgggga tgatcgccaa cgttttgaac gagctgctca
     3061 gaatatcgga ggtaggcatt atccacttgg ggacgcttct ttcacctttc aacttcttcc
     3121 ccgtgtggtt ctattggttg ttttctggtg tggcgatgag gatttcccat cctcctatca
     3181 ggtgttattc gatgcttcta tttcccatca cctgccaaca gatgtttgcg ccgttgccgg
     3241 aagcatgctc acccgacaat tgattgaatc tgaataatat acaaataaaa tttttatgcg
     3301 gaactatcta agattgatac ttttcgtcct actattcgcc ggggtgctaa atacaagctg
     3361 tgcaggaaac caggggatta gcaatcctga aactgcgaca gcacaggctg gtcgtattat
     3421 tggtttggca acccaaatgg gaggatacgc ccaggcaacc cagatggctg aaaatctcca
     3481 agtgacggcg accgctgctg ccaggcagct cttgctagat gaagtggggc agtggcgcct
     3541 cctgctttca gactcatatg atgaagacct aaacgattgg agcacaggga gtgatgaaga
     3601 tccggagctg ggttcgataa gctgggtgat tgaagacgga aaatatcgct ggcaagggct
     3661 cgcaaccaac ggttttgtgt ggtgggtcat tccagatatt gaaagcgttt cggatttcta
     3721 tctatcggtc tctgcccggc aggaggccca gcctgaagtg ggtgaatatg gtctggtgtt
     3781 ccgccaatcc ggagatgtag actattacct gtttgagatc agtgatttag gcctgtattc
     3841 cctgtatctg catactgaca gtggttggga atcattgatc gattggacag ctcatccttc
     3901 tatcaatcct ggtgcaacaa accgtctagc ggtgattgcc acaggcgagc attttgatct
     3961 ctttataaac gatacttttg tggctgaata tagcgacgac cggttacctg aggggaaggc
     4021 gggcttgcta gtaggcctct ccaatcccgg ggatgctgcg aactgggaat tcgatgattt
     4081 tgaatttcga gccccatgag gggaggtcat tgattaacct tttcatcagg tattctgtat
     4141 gtataatatc gatataagtt acgcggttgg aggaaaataa accccaacgg tgggactttt
     4201 gtatcgattt ataattccaa tatgatgccc taagggagac cagtaatgaa aaagtttcgc
     4261 gtggctctgc tggcaaattt gaaggtcaat gctccaaagt ttgaaggtat gccggaagac
     4321 cagtgggatg atttggattc agaaaaaacg atcaatgcgc tggtcaacgc cattcgggca
     4381 ggaggaaatt cctgcgagtt cctggaaggg aatctctcgc tggtagattc attgcagaaa
     4441 tttaaacccg atatctgctt taatatctgt gaaggacatt tcggggacgc acgcgaggcg
     4501 catgtgccag cgttacttga gatgctgcgc ataccataca ctggctccag agtgcttaca
     4561 ctcgctctgg cgctggataa acccatgacc aagcgtgtcc tggcttatca cgacctgcct
     4621 acgccgccat ttcaaacctt tgagcgggtg gatgagcctt taagcaccga tatgaactac
     4681 cctctgttcg ttaagcccag tcgagaaggc acgggtatgg gtgtttcagg caggtctatc
     4741 gtgcgtgatg aggatgaact gcgcgagcag gtggacttca tccttaaacg ctacaaacaa
     4801 cctgcactgg ttgagggcta tattgaaggg cgcgaggtga ccctcggagt ggtgggtaac
     4861 ttgatcgggc ctgtcgccag gcgcttgccg gacgatgatg a
//
LOCUS       DBNHOH010000670         1431 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_809545, whole genome shotgun sequence.
ACCESSION   DBNHOH010000670 DBNHOH010000000
VERSION     DBNHOH010000670.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_809545"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="AC16V7_11650"
     CDS             <1..410
                     /locus_tag="AC16V7_11650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6257819.1"
                     /translation="QIFLGARVEDHLLFPGQVGHHDLPRYYRMADLYLSASHSDGTSI
                     SLLEAMACGSPALVSDIPGNREWVQPGINGWLFPDGDEQALAESILKAFENRQQLPEM
                     GRRARQIAEQRADWDQNFPVLLHAYAIAMGNTK"
     gene            416..>1431
                     /locus_tag="AC16V7_11655"
     CDS             416..>1431
                     /locus_tag="AC16V7_11655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR6257820.1"
                     /translation="MTALREPFLIKGEIDRIFLILFLVINLLVLVNNVLHNPVVGYDG
                     TEHLKYIRVLPYRLPDTADTREFFSAPLPYVFPSLIDKACLRSKPVNSGSIAIDNCLI
                     FAGKFAQLINFVLSLGVTFCVGSIARIMQPESRWGEISALALLGVLTVYYKTFTQVRG
                     EPYLILFTAWTLYLVARMIQAPEQVRWRSGLKLGVVIGLALLSRQWGFMLLPALIGLF
                     ALVWLLDSHWGREWAKAMTVAALVAFLICGWFYLSLYIRYGTFMAFNRIPLVFSFSNQ
                     PSTFYRNTGLKNLLLFKSPIRGTFNNQLFPILYSDTWGDYWGHFVFIQDRSYLGEMGY
                     GNIQ"
BASE COUNT          303 a          384 c          362 g          382 t
ORIGIN      
        1 gccagatttt cctgggtgcc agagttgaag atcacctcct cttcccaggt caggtaggtc
       61 accatgacct gccccgctac taccgcatgg cagacctgta cctgagcgct tcgcacagcg
      121 atggtacttc catctcctta ctggaagcca tggcttgcgg gagtcccgcc ctggtctccg
      181 atatcccagg caaccgcgaa tgggtgcagc cgggtatcaa cggctggctc ttccccgatg
      241 gagatgagca agccttagcg gagtccatcc tgaaggcgtt tgagaaccgc cagcaattac
      301 ccgagatggg tcgccgagct cgccagatcg ccgagcagcg cgccgattgg gaccagaatt
      361 tccctgtgct actgcacgca tatgcaattg ctatgggcaa tacaaaataa acctaatgac
      421 tgctttgaga gagccctttc ttatcaaagg tgagattgat agaatatttc ttatcctctt
      481 cctggtaatt aacctgttgg tcttggttaa caacgtgctc cacaacccag tggtcggata
      541 cgatggcacg gaacacctca agtatatccg tgtgctgcca tatcgattac cggatactgc
      601 cgatacacgc gagttcttct cggctccgtt gccttatgtg ttcccctcgc tgattgataa
      661 agcctgcctc cgctcaaaac cagtcaacag cggatcaatt gctattgaca actgtctgat
      721 attcgctggc aagtttgcac aactcatcaa tttcgttctt tcattggggg tcaccttttg
      781 tgtcgggagt atcgcccgga tcatgcagcc tgagagccgt tggggggaaa tctcagcact
      841 ggctttgctg ggggtattga cggtatatta caagaccttc acccaggtgc gtggggagcc
      901 ttacctaatt ttattcacgg catggacgct ctacttagtt gctaggatga ttcaagctcc
      961 cgagcaagtg aggtggagga gcggcctgaa gctcggggtt gtcatcgggc tggcattgct
     1021 cagtcgccag tggggtttta tgctcctccc agcgctcatc ggactattcg ccctggtttg
     1081 gctcttggat agccattggg ggcgggagtg ggccaaagcc atgaccgtcg ccgctttggt
     1141 cgctttccta atctgtggtt ggttttatct gtctctctac atacgctatg gcacatttat
     1201 ggctttcaac cgcattcccc tggtattctc cttctccaat cagccaagca ctttctacag
     1261 gaatactggg ctaaaaaacc tcttgctgtt caaatccccg atccgaggca cttttaacaa
     1321 ccaactcttc ccgattttat attcggatac ctggggagat tactgggggc attttgtgtt
     1381 tatccaggat cgctcttacc taggagagat ggggtacgga aatatccagc a
//
LOCUS       DBNHOH010000671         2413 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_809586, whole genome shotgun sequence.
ACCESSION   DBNHOH010000671 DBNHOH010000000
VERSION     DBNHOH010000671.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_809586"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="AC16V7_11660"
     CDS             complement(<1..351)
                     /locus_tag="AC16V7_11660"
                     /inference="COORDINATES: protein motif:HMM:NF025670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HIR6257821.1"
                     /translation="MHTIGASNKKITRGLLFATLLLLALTLLVPSGLAAGPSPIAGQT
                     LMGGLSATGSSIPTLGIIQQWSLTAPEPGILDEFGFAVAIDGNTAVVGARNADPNLGG
                     GPIDDAGAAYVYIHN"
     gene            complement(731..822)
                     /locus_tag="AC16V7_11665"
     tRNA            complement(731..822)
                     /locus_tag="AC16V7_11665"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(786..788),aa:Ser,seq:gct)
     gene            complement(894..980)
                     /locus_tag="AC16V7_11670"
     tRNA            complement(894..980)
                     /locus_tag="AC16V7_11670"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(944..946),aa:Ser,seq:tga)
     gene            complement(1072..2235)
                     /locus_tag="AC16V7_11675"
     CDS             complement(1072..2235)
                     /locus_tag="AC16V7_11675"
                     /inference="COORDINATES: protein motif:HMM:NF027484.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HIR6257822.1"
                     /translation="MRADRQPLLMFLALMLVILSACTMPRRSTPTQSGSGLIYTAAAE
                     TVNAQLTLANQPPGTQFVAATFTPPGTFPSASPPPTQPLQTTASPVPTTTSVPCDRAK
                     FIDDVNFPDDTKINAGESFVKTWRLGNDGSCTWSTAYAVVFSGGDQMGAPVAVPLTGN
                     VSPGGTIDISVTLKAPDDGGTFRGEFKLRNASNVIFGLGTQNKPFWVQIRVPVATGLL
                     FDFLTQADAAAWTSGLGNDAGSALTFMGASDDANGAAKIVDQVKLETGATSGKILLMF
                     PKHGDNGYVTGLFPAYKVQSGDHLKARLGFMIPSGDSCGSGKVKFQIYSKEGDGELKL
                     LKEWSKSCNNSLLPIDLDLSSLKGKEVRFAFTVRAEGAFTDDWAIWNSPRIEH"
BASE COUNT          525 a          635 c          683 g          570 t
ORIGIN      
        1 attgtggatg tagacatatg ctgccccggc atcgtcgatg ggtcctcctc ccaggttggg
       61 atctgcgttg cgcgcgccaa ccacggcggt gttgccatca atcgccaccg caaagccgaa
      121 ctcgtccagg attccaggct ctggcgcggt cagcgaccat tgttggatga tccctagcgt
      181 ggggatgcta ctcccagttg ctgacagacc acccatgagc gtttggccag ctattggtga
      241 tgggccggct gccagaccgg aggggaccag cagcgtgaga gccagcaaca agagggtcgc
      301 gaataagagg cctcgtgtta tctttttatt cgatgcgccg atggtatgca tattctcctc
      361 cacccgaaag aattaaatga tgacttttac tctcgcatat aattattatc tcactgattt
      421 tagtccaata cgtgttgagc gtcaaacgta gtatgccaaa atgggggtta tgtgggacca
      481 ataaggctgg atgacacgcg agcttgcggt ttgttcttga attaatagat gtatataagt
      541 aagcggggca gatgccgaga ttcaccctgt ccagcgcgaa catccataca cgacagagtt
      601 ggtcgccctt attcgcggcg atttgcctgc agatagcacg gctcaccagt taagagactg
      661 gttgctgtct tcagacgggc agatgttgat cgaggagagt ggatacgtac cgcgatatta
      721 aagggggagg cggagagggc gggattcgaa cccgcggtag aggtttatag cccctacaac
      781 cgcttagcag gcggccccaa tcgaccactc tggcacctct ccgtgcgcgc cgagtattta
      841 cctcggcggc ttgaattcta ccatatttcc aattgtggtt gaggtgatgc aggcggaggg
      901 agagggattc gaacccccgg tgggcgtgaa cccacagctg ttttcaagac agccgcctta
      961 gaccgctcgg ccatccctcc taagcgatga gattctacca tagaggtcct ccccccacaa
     1021 aataggtcag aggcatcttt ggggttgcta tggagattcc caaagactcg ctcagtgttc
     1081 tattctcgga gagttccaga tagcccagtc atcggtgaag gcaccctcag cgcggacggt
     1141 gaaggcgaag cgtacctctt tgcctttcag tgaagataga tccaggtcga tgggcaataa
     1201 gctgttattg cagctcttgg accattcctt gagcagcttc agctcgccat ctccttcttt
     1261 ggagtagatc tggaatttta cctttcctga gccgcagcta tcgcccgagg ggatcatgaa
     1321 gcccaatctg gctttgagat ggtcgccgct ctgcacctta taggcgggaa acagcccggt
     1381 gacgtagccg ttatccccgt gcttggggaa catcagcagt attttgccgg aggtggcccc
     1441 ggtctcaagc ttgacctggt cgacgatctt ggcggcacca ttggcgtcgt cggaggcgcc
     1501 catgaaagtc aatgcgctcc ccgcatcatt tcctaagcct gaggtccatg cggcggcgtc
     1561 ggcctgggtc aggaagtcaa aaagcaagcc agttgcgacc ggtaccctta tctgcaccca
     1621 gaagggcttg ttttgagtgc ccaatccaaa gatgacatta gaagcgttgc gcagcttgaa
     1681 ttcaccccgg aaggtgcctc catcatccgg agctttcagg gtcaccgaga tgtcgatggt
     1741 cccacctggg ctcacgtttc cggtcagcgg cacggctact ggtgcgccca tctggtctcc
     1801 cccggagaaa actacagcat atgctgtgct ccaagtacag gagccgtcgt ttcccaaacg
     1861 ccaggtcttt acaaaacttt ccccggcatt gatctttgta tcatccggaa agtttacatc
     1921 gtctataaac tttgcccggt cgcatggcac cgaagttgtc gttgggacgg gagacgcggt
     1981 cgtctgaagt ggttgtgtgg ggggtggcga ggcgcttgga aaggtacccg gcggcgtgaa
     2041 ggtagcggca acgaactgag tgccgggcgg ctggttagcc aaggtcaatt gggcgttcac
     2101 cgtctcggcc gcggcggtat agattaaacc ggaaccggat tgggtgggcg tactcctgcg
     2161 cggcatggtg caggcgctca atatcaccag catcaaggct aaaaacatga gtaggggttg
     2221 ccggtcagct ctcatgcatt ccctcctcta gaatatgaga ccctaaaggt ctcgtaaacc
     2281 tttagggcct caaaatattt atgggatatc gaatttaacg tcttctgtga cgcaagtgcc
     2341 tttcaaatta ttctgcgtgc acaaagtgat tgtcgcctcg gctttgtgac cagatccgcc
     2401 atcctcgatg tta
//
LOCUS       DBNHOH010000672         2112 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_489660, whole genome shotgun sequence.
ACCESSION   DBNHOH010000672 DBNHOH010000000
VERSION     DBNHOH010000672.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_489660"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /gene="aepX"
                     /locus_tag="AC16V7_11680"
     CDS             <1..886
                     /gene="aepX"
                     /locus_tag="AC16V7_11680"
                     /EC_number="5.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003336797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050188 - phosphoenolpyruvate mutase
                     activity [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate mutase"
                     /protein_id="HIR6257823.1"
                     /translation="TTQFKQLLNSPQVEFLMEAHNGISARIVEEAGFKGIWGSGLCIS
                     AAMGVRDNNEASWTQVLEVLEFMSDATTIPILVDADTGYGNFNNVRRLVQKLEQRSVA
                     AMCIEDKLFPKTNSFIKGELQPLAEIGEFCGKIKAAKDIQQDPDFCVVARVEALITGQ
                     GLGEALRRAEAYYEAGADAILMHSKKSTPDEVLAFMKEWKDTCPVVIVPTTYFSTPTQ
                     VFAEAGVSVVIWANQLMRASIAAMQQTAAKLYQEQSLLEVETQIVKVKEVFRLQNADE
                     LNEAELRYLPMNGVHHNS"
     gene            1011..1643
                     /locus_tag="AC16V7_11685"
     CDS             1011..1643
                     /locus_tag="AC16V7_11685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6257824.1"
                     /translation="MRAFTKGGVTFKERHLSYCPRCNSKARHRRIWLFLKQKTNLFSD
                     RLRLLHVSPKYSLSRRFIRMPNLDYVGVDLNERPNISVKMDLTATPIRSAAFDAVICV
                     HVLEEILDDRKAMRELYRVMKSGGWALISVPTRMEQLTYEDPMIVTPDARQRAFGEVA
                     HVRIYGRDLVDRLKECGFQVQVDLAKDVPQQVREKYGLRDDENIFYCLKP"
     gene            1745..>2112
                     /locus_tag="AC16V7_11690"
     CDS             1745..>2112
                     /locus_tag="AC16V7_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257825.1"
                     /translation="MFTVALIGGDGAGKTTIAKQLEKSLPWPNRYLYMGQSTISSNAA
                     LPTSQVAKILKLRLDKRNGAQSGNSNPKAPSSNDLHYGSAKRSAIWVTARFMNRLAEA
                     WWRQLLTSYYLLRGYVVIYDR"
BASE COUNT          583 a          484 c          539 g          506 t
ORIGIN      
        1 aacgacccaa tttaaacagc tattgaactc tcctcaagtt gagtttctga tggaagcgca
       61 taatggcata agcgccagga tcgttgaaga agcaggtttt aagggtatct ggggaagcgg
      121 actatgcatt tcagctgcca tgggtgtgcg cgataataat gaagctagct ggacacaggt
      181 gctcgaggtc cttgagttta tgagtgatgc gaccacgatc cccatcctgg ttgatgcaga
      241 tacgggctat gggaatttta acaacgtgcg caggctggtc cagaagctcg aacagcgcag
      301 cgtagctgct atgtgcatcg aggataagct cttccctaaa accaactctt tcatcaaagg
      361 tgaactgcaa ccgttagcgg aaattggcga gttttgcggc aagataaaag cagcaaaaga
      421 catacaacag gaccctgatt tttgcgttgt ggcacgggtc gaggcactta tcactgggca
      481 ggggttgggg gaagccctca gacgggctga agcgtactat gaagccgggg cagacgccat
      541 tcttatgcac agcaagaaat caactcccga cgaagtgcta gcctttatga aggaatggaa
      601 ggatacctgc ccagttgtca tcgtcccaac aacctatttc agcaccccaa cccaggtatt
      661 tgccgaagcc ggggtaagcg tggtcatttg ggccaatcaa ctgatgcgag ccagcattgc
      721 agcgatgcag caaacagccg ccaagctgta tcaggagcaa tctcttttgg aggtagagac
      781 acagattgtt aaggttaagg aagtctttcg tttgcaaaac gcagatgaat tgaacgaggc
      841 ggaactcaga tacctaccga tgaatggggt tcaccataac agttgagatg agaaacacct
      901 atcggattgc tgacctgtcc ttgccccttc attgggctga agcactactc gctttccgag
      961 ctttactctt tgtcggcaca aagtacacct gcccctgctg tggatggaga gtgcgtgcct
     1021 tcaccaaggg aggcgttacg ttcaaggagc gtcacctcag ctactgcccg cgctgcaatt
     1081 cgaaagccag gcatcgtcga atatggttat ttctaaagca aaaaacaaac ttgttttcgg
     1141 atcgtttgcg attattgcat gtatcgccca aatacagttt atcccgacgc tttatcagaa
     1201 tgccaaacct cgattacgtc ggtgttgatc ttaacgaacg tccaaatata tccgtgaaaa
     1261 tggacctgac tgcgacccca atccgttctg cggcattcga tgcggtgatc tgcgtgcatg
     1321 tcttagaaga gatcctggat gaccgtaaag cgatgagaga actctatcgg gtcatgaaat
     1381 ctgggggctg ggcgctcatt agtgtaccta ctcgcatgga acaattaacg tatgaagatc
     1441 caatgatcgt tacaccagac gcgcggcagc gcgcatttgg tgaagtcgct catgtcagga
     1501 tttatggacg tgacttagtc gaccgactaa aagaatgcgg tttccaggtt caagtagatt
     1561 tggcaaaaga tgtgccccaa caggtccgcg aaaagtatgg cttacgtgat gacgagaata
     1621 tcttctattg tttgaaaccg tgatcaagat gtactgttag atttaagaat tacgcagctg
     1681 aaggaataag atcgtcaact gcggaacttc taagattaat gtttggaact agtaagaggt
     1741 atttatgttt accgttgctt taataggagg agatggcgct ggaaaaacga ctattgccaa
     1801 gcagcttgaa aagtcccttc catggccaaa tcggtacttg tatatgggtc agagtaccat
     1861 atccagcaac gcggctttgc ccacatcaca agtagcaaaa atcctgaaac tgcgactaga
     1921 caaaagaaat ggcgctcaat ctgggaacag caaccccaaa gcgccatcct ctaatgactt
     1981 acactacgga tcagcaaaac ggagcgcgat atgggtcaca gcgcgtttca tgaaccgttt
     2041 ggcagaagcc tggtggcgtc aactgctcac ttcatattac ctacttcggg gctatgtggt
     2101 gatatacgac cg
//
LOCUS       DBNHOH010000673         3162 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_297082, whole genome shotgun sequence.
ACCESSION   DBNHOH010000673 DBNHOH010000000
VERSION     DBNHOH010000673.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_297082"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="AC16V7_11695"
     CDS             <1..442
                     /locus_tag="AC16V7_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257826.1"
                     /translation="FGAWLLAATMNAMLTWWGVSIALLQHESLGNAVISREQLLRVVP
                     IFVAVMVWLIRVLIIGTFSVAGDRLFSQADKRVRGQSPFSLRNTRVRQVPQNAHISAK
                     SAQAFNPAPKPSPGDVVYSRPEPTYHPVSMQARSARNESTIIRR"
     gene            complement(486..1922)
                     /locus_tag="AC16V7_11700"
     CDS             complement(486..1922)
                     /locus_tag="AC16V7_11700"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6257827.1"
                     /translation="MSLRTRLAVLYTSIVGGILFLFGTAVYGAVSFSLISQVDGLIQN
                     TVEQIWPNIYVDSQGVLGVRNSPDLELAPGLLFQIWGRQEQLIYTNTPQIITPLDSIG
                     IEYSEPVFRYSTLNSTIGSVHLRVITVPMVIGAGGRKAGSLQVASSLAVIDTTQLALL
                     WVLVVGSVSAMGIAGIAGWMSTRQALAPLEGVTQVATQITKADDLSRRIPYQGPSDDE
                     VGQLIQAFNQTLGRLENLFNTQRRFLADVGHELRTPLTVIRGNVDLMRRMDSIDIESI
                     DSIENEVDRLTRLVGDLLLLAQAESGKLPLNIRLVELDTLLLDVMQQMRVLTHNRLNL
                     KLGEIDQVLVCGDQDRLKQVFVNLIGNAIQYTPEGGDVVVSIGKVGERGRVVITDTGQ
                     GIPQEDLPHIFERFYRGSKSRTRQKDGKGYGLGLSIAYWIIRNHRGQIDVDSQPGMGT
                     SFYVWLPLAHDDCVTSTTEAAQGDTNNK"
     gene            complement(1929..2606)
                     /locus_tag="AC16V7_11705"
     CDS             complement(1929..2606)
                     /locus_tag="AC16V7_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006102617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6257828.1"
                     /translation="MSSRILVIEDDEAILKFLRRGLAYEGYQVDTAADGTIGLNIARD
                     NPPDLVVLDLMLPGIDGLEICRRLRAGGPVPILILTAKDTVNDRIMGLDMGADDYMVK
                     PFNLDELLARIRALLRRSQPSRPQVLRFADLSLDTGTRQASRGERIISLTAKEYELLE
                     LFLRHPRQVLTRDMIFDRVWGYDFGGESNIIEVYIRYLRQKLEEINEPRLIHTVRGMG
                     YVLRESN"
     gene            complement(2722..>3162)
                     /locus_tag="AC16V7_11710"
     CDS             complement(2722..>3162)
                     /locus_tag="AC16V7_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012462946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR6257829.1"
                     /translation="VPLYVQAGADNITVHVEASRHLHRTIQYIKEMGVKAGVTLNPAT
                     SLSTLDEIISAVDLVLVMSVNPGFGGQSYISSSTDKITRLRHMLDARGLSHVDVEVDG
                     GIRPSNVPEIVRAGANVLVCGTSVFNSAASIEENIAALRAAIPG"
BASE COUNT          802 a          839 c          742 g          779 t
ORIGIN      
        1 gtttggagca tggcttttag cagccacgat gaatgcgatg ctgacctggt ggggtgtatc
       61 catcgcgctc ttgcaacatg agtcactcgg aaatgcagtg atttccagag aacagctact
      121 gcgtgtcgta ccgatatttg ttgcggtgat ggtttggttg atccgtgtgc tgatcattgg
      181 gacattctcg gttgcaggcg atcgattgtt cagccaggct gataagcgtg taagagggca
      241 atctccattc tcattacgaa atacaagggt gagacaagta ccccaaaatg cgcatatatc
      301 tgcgaaatca gctcaagctt tcaacccagc accaaaacca agtccaggag atgttgtcta
      361 ctctcgccct gaaccaacct accatccagt ctcaatgcag gctagatcag caagaaatga
      421 atcaactatt attagacgct aaaaacacaa acaaaatgac cctgaagatg agtatttctt
      481 cagggtcatt tattgtttgt atcaccctgc gccgcctcgg ttgtgctagt cacacaatca
      541 tcatgagcta gaggcaacca tacataaaat gaagtgccca tcccaggttg agaatcgaca
      601 tcaatctgac ccctatggtt gcgaatgatc cagtaagcta tcgagagacc taatccatat
      661 cctttgccat ctttttgacg cgtgcgcgac tttgatccac ggtagaagcg ttcaaatata
      721 tgggggagat cttcttgtgg tattccttga cctgtgtctg taatgaccac ccgtcctcgc
      781 tcacctactt tgccaatgct caccaccacg tccccaccct cgggtgtata ttgtatggcg
      841 tttccaatca aattcacaaa cacctgtttt aaccgatcct gatccccaca cacaagcacc
      901 tgatcgattt caccaagctt taaatttaga cgattatgcg tcaaaactcg catttgctgc
      961 ataacatcca acaacagcgt gtctaactca accaatcgaa tgttcagggg gagtttaccg
     1021 gattcagcct gtgcgaggag cagcaaatct cccaccaggc gggtcaaacg atccacctca
     1081 ttctcaatgc tgtcaatcga ttctatatct atgctatcca tcctgcgcat caagtccaca
     1141 ttaccgcgaa tgacggttaa gggcgtgcgc agttcatgcc ctacgtcagc cagaaagcgc
     1201 ctctgcgtat tgaataagtt ctccaagcgt cccaatgttt ggttgaaagc ctggatgagc
     1261 tgccctacct catcatctga tggaccttgg tagggaatcc gcctcgaaag gtcatcggct
     1321 ttggtgatct gtgtcgccac ctgtgtgacg ccttccaacg gggctaaagc ctggcgagtg
     1381 ctcatccatc ctgcgattcc agcgattccc atcgctgaaa cgctacccac aactagcacc
     1441 cacaaaagcg caagctgtgt agtatcgatc accgctagac tggatgccac ttgcaaggat
     1501 cctgccttac gccctcccgc accgatcacc atcgggactg taatcaccct aagatgtacg
     1561 gatccaattg tgctgttcaa tgtagaatag cgaaataccg gttcgctata ctctatcccg
     1621 atactatcca aaggtgtaat aatttgggga gtattggtgt atatcagctg ttcctgacgc
     1681 ccccaaattt ggaacagcaa tccaggggct aattcaaggt ctggcgaatt tctgacccct
     1741 aagacacctt ggctatcgac gtaaatattc ggccagattt gctccactgt attttgaatc
     1801 agtccatcca cctgtgaaat caggctgaag ctcactgctc cgtataccgc agtgccgaaa
     1861 aggaaaagaa ttccccccac gatagaagtg tagagaaccg ctaggcgggt gcgtagagac
     1921 attcaagttt agtttgattc gcgaagtaca tatcccatcc ccctgactgt atgaatcaat
     1981 cgaggctcgt tgatctcttc caatttctgc cgcagatagc gaatatagac ctcgataatg
     2041 ttgctttcgc cgccaaaatc gtagccccaa acccggtcga agatcatatc gcgggtcaac
     2101 acctgccgag gatggcgtag aaacagctcc aacaactcgt actctttggc agtcaaagag
     2161 atgattcgct ctcctcggga ggcctgacgc gtgccagtat ctaaggagag gtccgcaaat
     2221 cgcagtactt gaggtcggct gggttgagag cgacgaagga gagcgcgtat gcgcgccagc
     2281 agctcgtcca ggttgaaggg cttgaccatg tagtcgtcgg cgcccatgtc taagcccatg
     2341 atgcgatcgt tgacagtgtc cttggctgtt aggatcaata tcggtacagg cccgccagcc
     2401 ctgagccgcc ggcatatctc tagcccgtcg atgcccggga gcattagatc cagcacgacc
     2461 agatctggcg ggttatcgcg ggcaatgttc aatccaatag tgccgtctgc tgctgtgtca
     2521 acctgatagc cctcataagc caatccccgt ctcaagaatt tgaggatagc ctcgtcatct
     2581 tcgattacta gaatacgcga gctcatactg ttctcctgtt cacaatatac cacaaggcat
     2641 tatcagaaaa atgtgggtca tgggaaatgc tgtggtgaga ggttgcctta tgcatggaaa
     2701 tttgtcataa acttaatagg tttatcctgg gattgctgct cgaagcgcgg caatattctc
     2761 ctcaatgctc gcagcggaat tgaagaccga tgtaccacac accagtacat ttgcgccagc
     2821 ccgcacgatc tcgggaacgt tgcttggacg gatgcctcca tcgacctcta catcaacatg
     2881 cgacaggccg cgggcgtcca gcatgtgacg caaacgggtg atcttatcag tgctgctgga
     2941 gatataactc tgtcccccaa acccggggtt gacagacatc accagaacga ggtcaacagc
     3001 ggatatgatc tcgtccagag tgcttaaaga tgtggcggga tttagggtaa cgcctgcctt
     3061 cactcccatc tccttgatat attggatggt gcgatgcaga tgcctggagg cttcaacgtg
     3121 aactgtgatg ttatccgcgc cagcttgcac atataaggga ac
//
LOCUS       DBNHOH010000674         1178 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_361047, whole genome shotgun sequence.
ACCESSION   DBNHOH010000674 DBNHOH010000000
VERSION     DBNHOH010000674.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_361047"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="AC16V7_11715"
     CDS             complement(<1..389)
                     /locus_tag="AC16V7_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257830.1"
                     /translation="MPTILAIILLGLVAGAIVNYLADVLPIKRQLVAPICLNCESRQN
                     LVNYLVLPRRCEVCGQPRNIRSYVVDVVFALSAAWLWQSHPEDIGFVSGMLLLTFFGV
                     VTVIDIEHRLILNPVSLVGAIIGLSVGT"
     gene            517..>1178
                     /locus_tag="AC16V7_11720"
     CDS             517..>1178
                     /locus_tag="AC16V7_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR6257831.1"
                     /translation="MDKFIIQGGVPLTGQVTPSGNKNAALPLLAACTLTEEPVILHNI
                     PQIRDVNTMCLLLESLGVQIDKIDDNSWRIQAKHVRPADLDPNLCKQIRASILLAGPM
                     VARSGLLELPPPGGDIIGRRRVDTHILALKALGAQADFHRDEHIFRFSAKKLTGANIL
                     MDEASVTATENAIMAAVTASGTTVIRNAASEPHTQELCSFLNVMGAKIENIGSNTLEI
                     HGV"
BASE COUNT          354 a          284 c          258 g          282 t
ORIGIN      
        1 gtgcctacag acagtccaat tatcgcgccg actaagctca ctggatttaa aattaaccta
       61 tgttctatat caatcacagt gaccacccca aaaaatgtca ataaaagcat cccgcttaca
      121 aatccgatgt cctctggatg cgattgccat agccatgccg ccgagagagc aaaaaccaca
      181 tcaacaacat atgaccgtat atttcgaggc tgcccgcaca cctcgcacct gcgaggcaaa
      241 accagataat tgaccaaatt ctggcgggat tcgcagttca aacagattgg cgcaacgagt
      301 tggcgtttta tcggcaaaac atcagccaga taatttacaa tagcacctgc taccagaccc
      361 agaagaataa tagctaaaat tgtgggcata aattagacct aattatttca tcctttgaaa
      421 attagtataa tgggcgtatt atagcagggt ctctggccag agtatagaca cagccagacg
      481 catccttctg aaaatcaatg attgccagga gattcaatgg ataaattcat catccaggga
      541 ggtgttcctc tgactgggca agtaaccccg tcgggtaata aaaacgctgc actacctctc
      601 cttgcggctt gcacactcac cgaagaaccc gtcatattgc acaatatccc gcaaattcgc
      661 gatgtaaata ccatgtgctt actattggag agcctgggtg tccaaattga caaaatcgat
      721 gacaatagct ggagaattca ggcaaagcat gtgcgtccag ccgacttaga tcccaacctg
      781 tgtaagcaga ttcgagcatc tattctgctg gcaggaccga tggttgctcg atcgggattg
      841 ttggaacttc ctccaccagg aggcgatatt attggcagga gacgtgtaga tacccatatc
      901 cttgccctaa aagcgcttgg ggcacaggct gattttcatc gggatgaaca tattttcagg
      961 ttctcagcca agaaactgac cggcgcgaat attttgatgg acgaggcaag cgtaactgca
     1021 actgaaaatg caattatggc cgctgtaaca gcttcgggaa caacggttat tcgcaatgct
     1081 gcttccgagc ctcacaccca agagttatgc agctttctca atgtaatggg ggctaaaata
     1141 gaaaatattg gctcaaacac gctcgaaatt catggtgt
//
LOCUS       DBNHOH010000675         2414 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_938929, whole genome shotgun sequence.
ACCESSION   DBNHOH010000675 DBNHOH010000000
VERSION     DBNHOH010000675.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_938929"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2414)
                     /locus_tag="AC16V7_11725"
     CDS             complement(<1..>2414)
                     /locus_tag="AC16V7_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6257832.1"
                     /translation="GTDLIFARQLVAERIAAIYPTLPTWAAPPVMIQPLSSTSRAMKI
                     GISSDQYDLMDLSMIAYWKIRARLLDVPGVANAPIWGERIKMPQVQVDLERMRAYNVS
                     LNEVMDVTADALDVGLLTYSEGSVIGTGGFIDTPDQRYGIRSVLPVITPEKLGEVPIN
                     DRIKPDGTPLLIKDIGDVVWDTWPLFGDAVINDGYGLMMIVEKLPWANTLDVTRGVEE
                     AIEKMKPGLTGIDIDTTIFRPATFIEQSIDNLTESLLIGALLVVVVLLFFLYEWRVAL
                     ISATIIPVSLMASLLVLNLRGVTINVMTLAGLAIAIGAVVDDAIVDVENIVRRLRRLR
                     KEGSTQSTASIVLEASLEVRGAIVYASLIEISALLPVFFLEGLSGAFFQPLASAYVVA
                     GLVSPLVALTVTPAWVLLLLSNAPIEHRQSPIIPWMHRIYDRVLERILLTPRLAYGTV
                     STIVLAGVVVWPFLGQELLPSFKERDFLMHWITKPGTSHPEMWRITARACRELLTIDG
                     VRNCGSHIGQALMADEPYGVYFTENWISIDPSVDYDETLAKVQEAVDGYPGLIRDVQT
                     YLKERIREVLTGSSDTVVIRIYGPDLHTLQDKADEVLAAIDGIEGIVDLKKELHMDTP
                     QIEVEVDLEAAQRVGLKPGDIRRATAFLVQGEEVGDIFVGGKTYDVNVWSTPETRNSL
                     TDIRELLIDTPRGGRVRLADVADIRIAPVPNVIEREGQSRKIDVSANVRGRDLGSVAK
                     DVEAALQGVGFPLEYHAEVLGEFAERQAASQRILLAGSVAVIAIFFLLYTSFENWRLA
                     TLTFFT"
BASE COUNT          579 a          705 c          622 g          508 t
ORIGIN      
        1 ggtgaagaaa gtgagcgttg ccaagcgcca attttcgaag gaagtgtaca gaaggaagaa
       61 gatagcgatc actgccacag atccagcgag aaggatacgc tgcgatgcgg cttgcctctc
      121 ggcaaactcg cccaatacct cagcgtggta ctccagcggg aacccgactc cctgcagggc
      181 agcttcgaca tctttcgcta ccgaacccag atctcgccca cgcacattcg ccgaaacatc
      241 aatttttcgc gactggcctt cacgctcgat gacgtttggc accggcgcga tgcgtatgtc
      301 ggcgacatcc gctaggcgca cacgaccacc tctgggcgtg tcgatcaaca gctcgcgaat
      361 atcggtcagg ctattgcgcg tttcgggtgt gctccacacg ttcacatcat aggtctttcc
      421 tccaacaaaa atgtcaccga cttcttcgcc ctgcaccaag aaggctgttg cccggcggat
      481 atcgcccggt tttagcccaa cacgctgggc tgcttccagg tcaacctcga cttcgatctg
      541 aggtgtatcc atgtggagtt cctttttcaa atccacaatc ccctcgatcc cgtcaatcgc
      601 agccagcacc tcgtctgcct tgtcttgcag cgtatgcaga tcagggccat aaatgcggat
      661 gacgacagta tcgctggatc ctgtcagcac ctcgcggatt ctttctttta gataggtttg
      721 aacgtcgcgg atcaaccctg ggtagccatc caccgcctct tgaaccttgg caagggtttc
      781 atcgtagtcg actgaagggt caatgctgat ccagttctct gtaaagtaga ccccataggg
      841 ttcatccgcc atcagggctt gaccgatatg tgagccgcaa ttgcgcactc catcaattgt
      901 caagagctcc ctgcaagccc gagccgttat gcgccacatc tccgggtgag atgtgccggg
      961 tttggtgatc caatgcatca ggaagtctcg ttctttgaac gaagggagca attcctgccc
     1021 gaggaatggc caaaccacca cgccagccaa cacgatggtg ctcacagttc catacgccag
     1081 gcgtggtgtg agcaggattc gctccaacac cctatcgtat atacggtgca tccatgggat
     1141 gatcggggat tggcggtgct caatcggcgc attggataag aggagcaaga cccaggctgg
     1201 cgttaccgtc aacgccacca agggagatac cagccccgcc actacatatg cactggccaa
     1261 tggctggaag aacgctcccg acaagccctc caggaagaag accggcagca aagctgagat
     1321 ttcgatcagc gaggcataaa cgatagcacc gcgcacttcg agcgaagcct ccaggacaat
     1381 gctcgccgtc gactgggtac ttccctcctt gcgcagccga cgtaggcgtc gcacgatatt
     1441 ttctacatca acgatggcat cgtcgacgac tgccccaatg gcgatcgcta accctgccaa
     1501 agtcattaca ttgatagtca cgccgcgcag gttgagcact agcagggagg ccatcaaaga
     1561 tacggggata atcgtggcgc tgatcaaagc cactcgccac tcatacagga agaaaagcag
     1621 cacgaccacc acaagaagcg cgccgatgag caaagactcg gtaagattat cgatcgattg
     1681 ctcgatgaag gttgccggtc ggaaaatggt ggtgtcaata tcgataccag tgaggccggg
     1741 cttcatcttt tcgattgcct cttccacacc ccgcgtcacg tccaaagtat tggcccaggg
     1801 aagcttctcg acaatcatca tgagaccata gccatcgtta atcaccgcgt ctccaaagag
     1861 tggccaggta tcccaaacca catcgccaat gtccttaatg agcaacggcg tgccgtcggg
     1921 ctttatgcga tcattgatag gcacctcacc cagcttttcg ggtgttatga ccggcaatac
     1981 ggaacgaatg ccgtagcgct gatcgggagt atcaataaag cctccagtgc caatgaccga
     2041 tccttccgaa taggttaata atcccacatc cagcgcgtcg gcggtaacgt ccataacctc
     2101 gttgagggaa acgttataag cccgcatgcg ttccaggtcg acttgcacct gaggcatctt
     2161 gatacgctcg ccccagatag gcgcattagc cactccaggc acatccagca agcgggctct
     2221 gatcttccaa taagcgatca tcgaaagatc catcagatca tattgatctg acgatattcc
     2281 aatcttcatc gcccggctgg ttgacgagag gggctggatc atgaccggtg gggcagccca
     2341 ggtagggagt gtggggtaaa tcgccgctat gcgctccgcc accagctgcc gtgcaaaaat
     2401 taggtcagtc ccgg
//
LOCUS       DBNHOH010000676         2105 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1067177, whole genome shotgun sequence.
ACCESSION   DBNHOH010000676 DBNHOH010000000
VERSION     DBNHOH010000676.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1067177"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="AC16V7_11730"
     CDS             <1..420
                     /locus_tag="AC16V7_11730"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HIR6257833.1"
                     /translation="AGAALCMRAALDDARLETGDIDYINAHGTSTPLNDKSETAAIKT
                     VFGERAYNIPISSTKSMTGHLLGASGALEAVICVNALQTQSAPPTINYETPDPDCDLD
                     YVPNSARSMKIDTVMSNSFGFGGHNASIILSRYLNGE"
     gene            424..1533
                     /locus_tag="AC16V7_11735"
     CDS             424..1533
                     /locus_tag="AC16V7_11735"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HIR6257834.1"
                     /translation="MPRFAHVTGWGMAVPNKVMTNFELSKFVETNNEWIIDRTGIRER
                     RIANDDETTASLATDAALRALDMANLDPSKVDLVIVSTSSPEHLFPATACLVQDRIGA
                     SKAGAFDLSAACTGFIYALNFAAQAVRTGNVDNVLVIGAETLSRLVNWKDRGTCILFG
                     DGAGAFVLQATNVPGGVLSAVMRSDGSGGDLLSVPAGGSRLPTSAATIAQGQHYIQMN
                     GREVYRFATRVMTQATQEAVQKANLQMEDVNLIIPHQANQRIIEAAARQLNFPMDQVV
                     VNIGNYGNTSTASIPIATCEAFQKGQLNSGDRVVFVGFGAGLTWGAAVVQWSGPILAR
                     RRRLPRSYRAWARTRSFLNRLTRYIEGLLWGRWLK"
     gene            1683..>2105
                     /locus_tag="AC16V7_11740"
     CDS             1683..>2105
                     /locus_tag="AC16V7_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HIR6257835.1"
                     /translation="MNTSRNRSSIIYLLLFVAIIAMVVYNFQQQATTQEVLAINQVAA
                     DIQSGIVERIVIESDNRLRIIRADGTEHISNKEDNATLVEQLKALGVTTEQLNSDRFK
                     IEIKPPSAWLGIATALGYILPFIILAGVFWFVFRQAQGS"
BASE COUNT          515 a          543 c          537 g          510 t
ORIGIN      
        1 gccggtgcgg ccttatgcat gcgcgccgcc ctagatgatg ccaggttaga aaccggcgat
       61 atagactata ttaacgctca cggcaccagc acccccctta acgataagag tgagacagca
      121 gccatcaaga ccgtctttgg agagcgagct tataacatcc cgatttcctc taccaaatcg
      181 atgacgggcc acttgctggg ggcttccggc gcactggaag ctgtgatctg tgtcaatgcc
      241 cttcagaccc agtctgcccc tcccaccatc aactatgaga caccagaccc agattgcgat
      301 ctggattatg tccctaattc tgcccggtcg atgaaaatcg acacggtgat gtctaattca
      361 tttggatttg gcggccacaa tgcctccatc atccttagtc ggtatttaaa tggagaatag
      421 aagatgccac gctttgccca cgtaacaggt tgggggatgg ctgttccaaa taaggtgatg
      481 accaatttcg agctatccaa atttgtggaa accaacaacg aatggataat cgatcgaacg
      541 ggcattcgcg aaaggcggat cgctaatgat gatgagacta ccgcctccct ggcaaccgat
      601 gcagccctgc gagccctgga tatggcaaac ctggatccct ccaaagttga tttggtgatc
      661 gtttccactt catcgccaga gcatctcttt cctgccactg cctgtctcgt ccaagaccgc
      721 atcggcgcca gcaaagccgg ggcttttgac ctctcagctg cctgcacggg cttcatctat
      781 gccctcaatt tcgctgccca agcagtacgt accggcaatg tagataatgt cctggtcatc
      841 ggcgcggaga cactctcgcg cctggtgaac tggaaggatc gcggcacttg catcctgttt
      901 ggcgatggag cgggggcctt cgtgttgcaa gccaccaatg tgccaggggg tgtgctctcc
      961 gcagtgatgc ggtcagacgg ttccggaggt gatctgcttt cagtacctgc aggcggtagt
     1021 cgattgccaa ccagtgcggc aaccattgcg caaggtcagc actatatcca aatgaatggc
     1081 agagaggtct atcgttttgc cactcgggtg atgactcagg caacgcaaga agctgtccaa
     1141 aaggctaatc tgcaaatgga agatgttaat ctaatcatcc cccatcaggc aaaccagcgc
     1201 atcattgaag cggctgctcg ccagctcaat ttccccatgg accaggtggt ggtaaatatt
     1261 ggcaattacg gcaacacatc aaccgcatcg atccctatcg ctacctgtga agcgttccaa
     1321 aaagggcagt taaattcggg ggaccgggtc gtcttcgtag gttttggcgc tggtctcacc
     1381 tggggagcgg cggtcgtgca atggagtgga ccaatcctgg cgcgcaggag gcgcttgcct
     1441 cgcagttacc gtgcctgggc acgtacgcgc tccttcttaa atcggctaac acgctatatt
     1501 gaagggttac tctggggaag atggctcaaa tagtgcaaag aatcactcgt atctgtcgag
     1561 aaatttgtta gaatgttgtt agagatttct aaaaatgcca cccctactcc tcccatcaat
     1621 ccccataggt tggtataatt taaactaatg aattgggatt gtctgataat tttggaggta
     1681 aagtgaatac gtcccgcaac cgatcatcta ttatatacct gctgctgttt gtggcgatca
     1741 tcgccatggt agtctataac ttccagcagc aggcaaccac gcaagaagtt ttagcgatta
     1801 accaggttgc tgccgatatt cagagcggga ttgtcgagcg gattgttatt gaaagcgata
     1861 accgattgcg aataatacgc gcagatggta cagagcatat ttctaacaag gaagataacg
     1921 ccacactggt tgaacaactc aaagcattgg gcgttacaac tgaacagctc aactcggatc
     1981 gtttcaaaat cgagatcaaa ccgcccagtg catggttagg tattgctact gcacttggat
     2041 acatcttgcc tttcatcatc ctggcgggtg ttttctggtt tgttttccgg caggcgcagg
     2101 gcagt
//
LOCUS       DBNHOH010000677         3142 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1643239, whole genome shotgun sequence.
ACCESSION   DBNHOH010000677 DBNHOH010000000
VERSION     DBNHOH010000677.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1643239"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /gene="gpmA"
                     /locus_tag="AC16V7_11745"
     CDS             <1..639
                     /gene="gpmA"
                     /locus_tag="AC16V7_11745"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HIR6257836.1"
                     /translation="HTAAKLLAEEGFTFDVAYTSVLKRAIKTLWIVLEDMNLEWIPVY
                     RNWRLNERHYGALQGLNKAEMAVEYGEEQVKIWRRSYDTPPPALDWDDSRHPRFDARY
                     ADLTKDDLPACESLKDTVARMLPYWHETIAPAVKSDQRVLISAHGNSLRALVKYLDDI
                     SEKEIVELNIPTGIPLIYELDDNLKPLKSYYLGDAEAIRLAAEAVAKQGQAR"
     gene            complement(683..1408)
                     /locus_tag="AC16V7_11750"
     CDS             complement(683..1408)
                     /locus_tag="AC16V7_11750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HIR6257837.1"
                     /translation="MNAQSQSSTLNPQVIKALSWLVTLLMPVVLVMAAVRLLLTPAFL
                     IIEYNTPGFPPDPYGFTKSERLTWSKIAVEYLLNDADINFLGDLRFPDGKQAPAESCR
                     AMDDCTRLYNDRELKHMLDVKVVTQAALRVWYISLAVLLLLYIWAWRGGWWGAFRQGI
                     GRGGWLTVFLLAGIILFVILAFGVIFVAFHNVFFEAGTWQFLFSDTLIRLFPERFWRD
                     TFIAIGVLAGGTGLALGYFLREK"
     gene            complement(1405..2946)
                     /locus_tag="AC16V7_11755"
     CDS             complement(1405..2946)
                     /locus_tag="AC16V7_11755"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HIR6257838.1"
                     /translation="MYFAVFASGMTTLAIELSASHLLGSVFGTSNLVWASIIGLILIY
                     LTAGYFIGGRWADRSPHERTMYTVMAWGAFTSGLVPVAAYPVLRLAADSFDQLQMGVL
                     FGSFAAVLVLFILPVTLLGTISPFAIRLAIRDTQSAGVTSGRIYAISTLGSFVGTFLP
                     SLILIPLVGTRRTFLIFSAFLVLTAFGGLWRSSGWRGVLRLLWMPVLLILLDILGAGR
                     PIKSTSGQIFETESAYNYIQVLERDGYRLLRLNEGQGIHSMWHPTQIAYEGPWEQFLV
                     APFFNPPRYEPERVASMAILGLAAGTTARQATAVFGAIPLDGYEIDPKIIEVGREYFG
                     MTMPNLNPIAQDGRVGLARSPKRYTVIGIDAYRPPYIPWHLTTREFFQQVQEHLTDDG
                     TLVINVGRSPTDRRLIDGLVGTISSIFPSIYVIDVPNTFNSIVYATNQPTNIENLYEN
                     WLYLKTRQDVHPLLLHAIELAIIHQQPTPESDIVYTDDLAPVEWVTNSMILNFVLKGD
                     MNQLR"
BASE COUNT          876 a          825 c          767 g          674 t
ORIGIN      
        1 catacagccg ccaagttgtt ggcagaagaa ggctttactt ttgatgtggc atatacttcc
       61 gtgcttaaac gggctattaa aaccctttgg atcgtgctcg aagacatgaa cctggaatgg
      121 attccggttt accgtaactg gcgcctgaac gagcgacact acggcgccct gcaaggcttg
      181 aataaggctg agatggctgt cgaatatgga gaggagcagg tcaagatttg gcgcagaagc
      241 tatgatactc cacctcctgc cctagattgg gatgattcac gccacccgcg ctttgatgcc
      301 cgctacgctg atctcaccaa agatgatttg cccgcctgcg aatcattgaa agacaccgtg
      361 gcgcgcatgc tgccatactg gcacgagacc atcgctccgg cggtaaagtc agaccagcgc
      421 gtcctgatat ccgcccacgg caatagccta cgcgctttgg tcaaatacct ggacgatatc
      481 tccgaaaaag agattgtcga attgaatatc cccactggca tccccttaat ttatgagttg
      541 gatgataacc tgaaacccct caagagctac tacctaggag acgcggaggc gatccgtttg
      601 gcagcagaag cagtcgccaa gcagggacag gcgagataat accgatgata caagcttggg
      661 gcggtgatac cgccccaagt gtttatttct ctcgaaggaa atagcccaaa gccagcccag
      721 tcccgccagc tagtacaccg attgcgataa atgtgtcccg ccaaaatcgc tccggaaata
      781 ggcggatcag ggtatcgcta aacaggaatt gccatgtgcc cgcctcaaag aagacattgt
      841 gaaatgcgac gaagatcacc ccgaacgcca agatcacaaa taggataatc cctgcaagca
      901 gaaacacagt caaccaaccg ccacgcccta taccctgccg gaatgccccc caccagccac
      961 cacgccaggc ccaaatatat aagagtagca acaccgccag gctgatatac cacacgcgca
     1021 atgccgcctg agtaacgacc ttcacatcca gcatgtgttt cagctcgcga tcgttataca
     1081 gacgggtgca atcatccatc gccctgcaag actctgcggg cgcctgcttg ccatcgggaa
     1141 accgtaagtc acctaaaaaa ttgatgtctg catcattgag caggtattcg actgcaatct
     1201 tcgaccacgt taaccgctca gatttcgtaa agccatacgg gtcaggcgga aatccagggg
     1261 tgttgtactc aatgatcaaa aaagcggggg taagcaacag gcgaactgct gccatcacta
     1321 aaacaaccgg cataagcagg gtaaccagcc aggagagtgc tttgatcact tgggggttta
     1381 atgttgaaga ctgggattgg gcattcatcg cagttggttc atatctccct tcagtacaaa
     1441 attgaggatc atggagttgg ttacccactc aaccggggcg agatcatctg tatatacgat
     1501 atcactttcg ggtgtaggct gctgatggat aatagctagc tcaatggcat gcaacaagag
     1561 ggggtgtaca tcctggcgag tttttaaata caaccagttt tcatataggt tttcaatatt
     1621 ggtcggctgg ttggtagcat atacaatcga attgaatgta ttcgggacat ctatcacgta
     1681 gattgaaggg aaaattgagg aaattgtgcc cactagcccg tcgatcagac ggcgatccgt
     1741 gggcgagcga ccaacattga tcaccagggt tccatcatcg gttaagtgct cctgcacttg
     1801 ctgaaaaaat tctcgcgttg tcaagtgcca ggggatatat ggtggtcgat aggcgtcaat
     1861 accaattact gtgtaccgct ttggactgcg cgccagtccc acgcgcccat cctgggcaat
     1921 aggattcagg ttgggcatcg tcatgccgaa atattctcgg cctacctcaa ttatcttcgg
     1981 atcgatttcg taaccatcaa gagggatggc tccaaaaacc gctgtcgcct gcctggcagt
     2041 cgtgcctgcc gccaatccca agattgccat actcgctact cgctccggct catacctggg
     2101 agggttgaaa aatggcgcta ccaggaactg ctcccaaggg ccttcatagg cgatttgggt
     2161 tggatgccac atggagtgga taccttgtcc ttcattcaaa cgcaacaagc ggtaaccgtc
     2221 cctctccagt acctgaatat agttataagc cgattcggtc tcaaagatct ggcctgaggt
     2281 ggattttatg ggtcgccctg cacctagaat atccagcaat atcaataata ccggcatcca
     2341 caggaggcgc agaacacccc gccagccaga tgaacgccag agtccaccaa aggcagtgag
     2401 aaccaagaac gcgctgaaga taagaaaagt acggcgcgtg ccaaccagag gaattaatat
     2461 caaggacggt aaaaatgtgc ccacaaatga gcccagggtg gagatggcat agatgcgccc
     2521 agaggtcacc cctgcgctct gtgtatcacg gatcgccaga cgaatggcaa atggagaaat
     2581 cgtacccagc agcgtaaccg gtaagataaa aagcactaaa acagcagcaa aagaaccaaa
     2641 tagcacgccc atctgcaact gatcgaagga gtctgccgcc aggcgcaaaa ccgggtaagc
     2701 ggctaccgga actaaaccag aggtgaatgc cccccaggcc ataacagtat acatcgtcct
     2761 ctcatgcggt gagcggtctg cccagcgccc cccaataaaa tagccagcag tcaagtagat
     2821 taagatcagg ccaataatgc tcgcccagac caggttgctc gtgccgaaaa cactgcccag
     2881 caggtgtgaa gcgctaagtt caatcgccag ggtagtcata cccgaagcga agacggcgaa
     2941 atacaaatat catgtcataa ttagtacctg attatcccct atcttcaccc tgcacgcaag
     3001 gtaaggtcat ctctcactgt gtttttttta acgtgatttc cgactagccc aatttatttt
     3061 catcacttat aatagctcaa accgagccca tatttcgatg gaggccaaaa tgctaaagga
     3121 atttaaagaa tttgatatgc gc
//
LOCUS       DBNHOH010000678         7582 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1003069, whole genome shotgun sequence.
ACCESSION   DBNHOH010000678 DBNHOH010000000
VERSION     DBNHOH010000678.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7582)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7582)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7582
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1003069"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="AC16V7_11760"
     CDS             complement(<1..994)
                     /locus_tag="AC16V7_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR6257839.1"
                     /translation="MTKQLTKQTIGAGVAGTGFIGPAHIEGLRRNGIQVLGLAEITKA
                     KAEQKATELGIPRLYGSLDDMLADPDIDVVHLATPNYLHHSHAKAALLAGKHVVCEKP
                     LAMNATESAELVELAAERRLVNSVNFNIRMYPLVQQARSIVQNGELGDLFILQGSYLQ
                     DWLLFPTDWNWRLEPSLGGTLRAVGDIGSHWFDLLTFITGLRVEEVYADFKTFHPIRK
                     KPAKPLETFTGKILTPEDYIDQPIHTEDYATILLHYEKGVRGVLTVSQVSSGRKNRLF
                     YEINGSKSSLGWNSERPNELWIGHRTEPNQTLMKDPSLLSLEARETASYPGGHNE"
     gene            complement(1007..1747)
                     /gene="deoC"
                     /locus_tag="AC16V7_11765"
     CDS             complement(1007..1747)
                     /gene="deoC"
                     /locus_tag="AC16V7_11765"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIR6257840.1"
                     /translation="MTLNSKTVTYEQLAKVIDHSLLRPELTEADIVAGCELAARYHTA
                     TVCVKPCHVKLAKEILKDSDVLVSTVVGFPHGSNLTAIKVAEAQQAMDDGAVELDMVL
                     NIGQLRSGKTDYVRDDIKEVCDAAHRRSVKVKVIFENAYLTDEEKITACKLCEEAGAD
                     WVKTSTGFAPSGATLDDLRLMRANVSQKVQVKAAGGVRTLPDLLEVIDVGVTRCGATA
                     TATILDNFKALQEGKKVEIKPNATPGAY"
     gene            complement(1772..2851)
                     /locus_tag="AC16V7_11770"
     CDS             complement(1772..2851)
                     /locus_tag="AC16V7_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR6257841.1"
                     /translation="MSKTVKIGMIGTGFVGDIHHAAFKGWVHNADLVAVASPNNAANF
                     AKERGIPNAYSDYREILKDKEIDVVDIGIPNDLHCEAVVAAAQAGKHVIVEKPLCVTL
                     EEADEMIEACKKAGVLLMYAEELLFAPKYVRVKMLIDEGAIGEPFLAKQSEEHPGPHM
                     PWFWDVNRSGGGVLLDMGCHSIEYTRWVLGKPKVKSVTAFMGTYVHQGRTQGEDHSYC
                     IIEYEGNKVAMIENSWAKGGGVDDRCEIYGKKGHTRADLLRGSSLLTYSEEGYGYAVE
                     KAGQTKGYTFTMFEEIWNYGFPQEMQHFVDSVLGKVTCMETGEDGREVLKIMYAAYQS
                     AGEGRKIGFPYDPPKVKKPIDLWKR"
     gene            complement(3022..3990)
                     /locus_tag="AC16V7_11775"
     CDS             complement(3022..3990)
                     /locus_tag="AC16V7_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6257842.1"
                     /translation="MKRFYISPPVAALLLAVVLFVLSGLLPNSYGSDLNVARSQALNI
                     LRLSVFLGVIAAGQTLVIISGFEGIDLSAGSIVTLTAIFTYVIVKGEDNKVLLALLVS
                     LAVGAFIGFLNGAGITFLKISPFVMTLGMSGVVTGAIILANHGNVSGKVAPIMTRVIA
                     RPLSPSFPIPNALIIWLIFGVLMWLLLERTAFGKNLFAIGVNRVTAKLSGVNVIAMNL
                     ATYTLAGLLAGFGGFLVVGNTGVVLIQLGQPFLFPSIAAVAVGGTLLSGGKGSYWGTM
                     AGAIVLTLITSLLTTMQMPDSVRRMVLGTTLLVLISIYGRQQALRQ"
     gene            complement(3987..4955)
                     /locus_tag="AC16V7_11780"
     CDS             complement(3987..4955)
                     /locus_tag="AC16V7_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6257843.1"
                     /translation="MKKRITWDFLITRYPAFFPLILLMLSIAINLFLQPNMFDLETLN
                     SNMRVFLPLILMAVGQAIVLLGGGIDISVGGIVSIVNTILATRVGLDGSPEKMWQFVL
                     ISLLAGVLAGAINGFFIAFLRLQPIITTYATSFLYAGFALFLLPNPGGGIPADIANLY
                     RTSTPLGLPLAFYVIAILLLIWIYVSSTRYGKYLFAVGGKADAAYETAVPVTLMQFST
                     YVISGFMAALAGIAITMLSGSGNADIGNPMTLNSITAVVIGGNAFSGGIGGVAGPVMG
                     AVTLSLIQNIISFAHIDTWWETLVKATIIVVALATPGIINLFRRRE"
     gene            complement(4952..6460)
                     /locus_tag="AC16V7_11785"
     CDS             complement(4952..6460)
                     /locus_tag="AC16V7_11785"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR6257844.1"
                     /translation="MAKSLSARNIVKRYGGVVALSDGNLDVQTGEVVALIGANGSGKS
                     TMSKVINGVVVLDGGQLLLDDKTVHFSSPQAAKNMGIATVFQELSLIPHMTVAENIWL
                     TREPMTPLGTVNRKAIIEQTEQLLSLFRGTYKTDLRPDDPVASLPPDEKQIIEILKAI
                     SFNPWMVILDEATASLDSRQVQRLFELVRNWKEVGKAIVFVSHRMEEIFRIADKYSVL
                     RNGKTVGAGNMEDINEKELVKLMIEKESVFTFTRGETKVNEKPVCLEVKDLRTDSLRG
                     VSFSVREGELVGIGGLRGQGQRDLLLSLFGDVPYSGQVCLFGNECRLRHPREAMKLGL
                     ALVPGDRSREGLLYIRSILENLQLPSWAQYGFPLRIGKAKKDAEQAGANLNLIMAGLG
                     EPVSSLSGGNAQKVVIGKWLMRKPKLLLLDDPTKGVDVGTKAEFYALLTQLCSEGKTI
                     LFYSSDDEELVGLCDRVLVIHDGIIRSELSGATLTKENLIAASLGGSNGGAL"
     gene            complement(6467..7528)
                     /locus_tag="AC16V7_11790"
     CDS             complement(6467..7528)
                     /locus_tag="AC16V7_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR6257845.1"
                     /translation="MCEGQPDAYLLDDIMTDEEVQQFFVQEGAAEPFTIGVSNPFISS
                     EYRTQMIASLIEVNQEYMDQGITTELVIESADTDVAGQIQQLQNLIAKDVDAILVNPG
                     DVNGLNATLLEALSKGIIVISVDQELNVPGVYNAGIDQKAWAMTSARWLADKLGGEGD
                     IVQIEGFPGHPANVARMEGVAEVFANYPGINVLATDTGRWDEATGQQVMSNFLAAYPN
                     LNGYWTQDGMAIGALQAVMAANPAQWPQGVGEGRCQYLKLWQEALTMNPDFDSIAVAN
                     HPGVSPTGLRIAVNMLQGKKVDESKLGGVNGLSFVIPLAAVITSENLDEGLAMCEGQP
                     DAYLLDDIMTDEEVQQYFK"
BASE COUNT         1745 a         2125 c         1946 g         1766 t
ORIGIN      
        1 cctcgttgtg acctccggga taagaagcgg tctctcgtgc ttcaagagag agcagcgaag
       61 ggtctttcat cagtgtctga ttgggttctg tccgatgacc gatccataac tcgttgggcc
      121 tttccgagtt ccaacccaac gaagacttgg aaccgttgat ctcatagaac aggcggttct
      181 tgcgccctga agagacttgc gagaccgtca gaacaccgcg cacacctttc tcgtaatgca
      241 gcaggatagt ggcgtaatct tcggtgtgaa tgggctggtc gatgtaatct tcaggcgtga
      301 ggatttttcc ggtgaaggtt tccagcggtt tggcgggttt cttgcgaatg gggtggaagg
      361 ttttgaagtc agcatagact tcttcaacgc gcaggccagt gataaaggtc agcaggtcga
      421 accaatggga gccgatgtcg cccacagcgc gcagagtccc acccagtgac ggttcaaggc
      481 gccagttcca gtcagtggga aagagcagcc aatcttgcag gtacgatccc tgcaggatga
      541 acaggtcacc caattcacca ttttgcacta tactccgcgc ttgttgaaca aggggataca
      601 tgcggatatt gaagttgacc gagttgacga gtctcctctc tgccgccagt tcgaccagtt
      661 cagccgattc ggtggcattc atcgccaatg gcttctcgca gacaacgtgc ttgccagcca
      721 gcaaagcggc tttggcatgc gagtggtgca ggtaatttgg cgtggcaaga tgcactacat
      781 ctatatcagg atcagccaac atgtcatcca gcgaaccata aaggcgcgga atgccaagtt
      841 cagtggcctt ttgttcggct tttgctttcg tgatttcagc caatcccaac acctggatgc
      901 cattgcgacg caagccttca atgtgagccg ggccaataaa gccggttcct gctacgccgg
      961 cgccgatggt ttgcttggtg agctgctttg tcattgtgga atctccttag tatgctccgg
     1021 gtgtcgcatt cggtttgatt tctactttct tgccttcctg tagcgcttta aagttgtcca
     1081 ggatggtagc cgttgcagtg gcaccacagc gggtcacgcc aacatcgatc acttccagga
     1141 ggtcgggcaa tgtgcggacg ccgcccgccg ctttgacctg taccttctgg ctgacgttag
     1201 cgcgcatcag acgcaagtca tccagagtcg cgccgctggg agcaaaaccg gtggaggtct
     1261 tgacccagtc tgcgcctgcc tcttcacaaa gtttgcaggc tgtaattttt tcttcatcgg
     1321 ttagatacgc gttctcgaag atgaccttga ccttcacgct acgcctatgg gcagcatcac
     1381 agacctcttt gatgtcatca cgtacgtaat cagtttttcc tgagcgtaac tgaccgatgt
     1441 tcaataccat atctagctcg accgcgccat cgtccatggc ttgctgggct tcggcgacct
     1501 tgatggcggt cagattgctt ccatgtggga atcccaccac ggtgctgacc aacacatccg
     1561 agtccttgag tatttctttg gcaagtttaa cgtgacaagg ttttacgcag acggtggcgg
     1621 tatggtagcg ggcagccagt tcgcaaccag cgacgatgtc cgcttcggtc aattccggac
     1681 gcaaaagaga atggtcaatg accttggcaa gctgttcata agtgacggtt ttgctattta
     1741 aagtcataac atggactcca aaatctccag attacctttt ccacaaatca atcggcttct
     1801 tgactttcgg cgggtcgtag gggaacccga tcttacgtcc ctcgccagcc gattggtagg
     1861 cggcatacat gatcttcaat acttcacgac catcctcgcc agtctccatg caggtgactt
     1921 tgccgagcac actatcaaca aaatgctgca tctcttgcgg gaagccatag ttccagatct
     1981 cttcgaacat ggtgaaggtg tagcctttgg tctgtccggc tttttccaca gcgtaaccat
     2041 acccttcttc tgagtaggtc agcaacgaac tgccgcgcag caagtcggcg cgcgtgtgac
     2101 cttttttccc gtaaatctca caacggtcgt ccacgccgcc acctttagcc caactgttct
     2161 cgatcatggc gactttgtta ccctcatatt cgatgatgca ataggaatgg tcttcgccct
     2221 gcgtgcgtcc ttggtggacg taagtgccca taaaagcggt cacgctcttg actttgggtt
     2281 tgcccagcac ccagcgagtg tattcaatcg agtgacaacc catatcgagc agcacgccgc
     2341 cgccggaacg attcacgtcc cagaaccagg gcatgtgcgg accgggatgc tcctcagatt
     2401 gtttcgcaag gaaaggctcg ccaatcgcgc cttcatcgat cagcattttg acacggacgt
     2461 attttggggc aaacagcagc tcttccgcat acatcagcaa aacgcccgct tttttgcagg
     2521 cttcaatcat ctcgtccgct tcttcgagcg tgacacacag cggtttctcg acgatgacgt
     2581 gtttgccagc ttgagcggcc gccactacag cttcgcaatg caggtcgttc ggaatgccga
     2641 tgtctaccac atcaatttct ttatctttta aaatctcgcg gtaatcgcta tacgcgtttg
     2701 ggattccgcg ctctttggca aaattagcag cgttattggg actggcaact gcaaccaaat
     2761 ccgcattatg cacccagccc ttaaaagcag catgatgaat atcaccgacg aaaccggttc
     2821 cgatcatacc aatctttacc gttttgctca tggcattctc cttccttcaa tgaatgttca
     2881 caggcctttg cctgaaagag ttatttgaga ataagcgagc gcgtcaatct gcaatggaaa
     2941 ctgcggtggg agctgcaatg aagtatccga tgtaagctct caaggggttg tgataggata
     3001 cgagccctgc ctatttccga attattgccg caaggcttgc tggcggccgt agatagagat
     3061 caatactagt agtgttgtgc ccaacaccat acggcggaca gaatcgggca tctgcatagt
     3121 ggtcagcaag ctggtgataa gcgtcaacac gattgcgccg gccatcgtgc cccagtagct
     3181 gcccttcccg cccgagagca gtgtcccgcc gaccgccacc gccgctatgg atgggaacag
     3241 gaacggctga ccaagttgaa tcaacaccac gcctgtgttc ccaaccacca ggaacccacc
     3301 aaacccagcc agtaaacccg ccagggtata ggtcgccagg ttcatcgcaa tcacattgac
     3361 gccggagagt ttagccgtca ctcggttaac accgatggca aacaggttct tgccaaaggc
     3421 cgtgcgttcc agcagcagcc acatcaggac gccaaaaatc agccagatga tgagcgcatt
     3481 cgggatggga aaactcggcg aaaggggtcg cgcaattacc cgggtcatga tgggggccac
     3541 cttcccgctc acgttgccgt gattggcgag gataatcgcc ccagtgacaa cgcccgacat
     3601 gccgagcgtc atcacaaacg gggatatttt caaaaaggtg ataccagcac cgttcaaaaa
     3661 gccaatgaaa gcgcccacag cgagggaaac gagcaaggcc agcagcacct tattatcctc
     3721 gcctttgacg atgacgtagg taaagatggc cgtcagcgtg acaatggaac ccgccgataa
     3781 gtcaatccct tcgaagccac tgatgatgac cagggtttgc cctgccgcaa tcacaccgag
     3841 aaagaccgac aggcgcaaaa tattcaaggc ttgagagcgc gcaacattta aatcgctacc
     3901 gtagctatta ggcagcaggc ctgagagcac gaatagcact actgccagca gcaatgcggc
     3961 gaccgggggt gagatataaa aacgcttcat tctcgcctcc tgaacaggtt gatgatgccc
     4021 ggtgtcgcca gcgcgaccac gatgatcgtc gctttaacca gcgtttccca ccatgtgtca
     4081 atatgcgcaa acgaaatgat gttctggatc aggctgagtg tgaccgcgcc cataaccggc
     4141 ccagccacgc cgccgatgcc accgctgaaa gcgttccctc caatgaccac tgcagtgata
     4201 gagttcagag tcatcggatt accaatatcc gcattgccgg acccacttaa catggtgatc
     4261 gcaattccgg ccagcgccgc catgaagcca gagataacat aggtgctgaa ttgcatcagc
     4321 gtcaccggca cggcagtttc gtaggctgca tccgctttgc cgcctacagc gaaaagatat
     4381 tttccatagc gggtggaact gacgtagatc cagatcagca ataagatggc gatcacatag
     4441 aaagctagag gtaggcccag gggcgtcgag gtgcgatata agtttgcaat gtccgcagga
     4501 atgccgcctc ctggatttgg caggaggaag agtgcaaacc cggcgtacaa gaagctagtt
     4561 gcgtacgtag tgataatggg ttgcaaacgc aaaaaggcga tgaaaaaacc gttgattgcg
     4621 cctgccaata cgcctgccag cagggaaatc agcacgaact gccacatctt ctccggcgag
     4681 ccatccaggc ctacacgagt cgccaggatg gtgtttacta ttgaaacaat tccaccgacc
     4741 gaaatgtcga tcccgccacc tagcagcaca atcgcctgcc ccaccgccat caggattagc
     4801 ggcaagaaga cgcgcatgtt actgttcaac gtttcgaggt cgaacatatt gggctgcagg
     4861 aacaaattga tggcaatgga cagcatcaac aaaatgagcg ggaagaacgc cgggtagcga
     4921 gtgatcagga aatcccaggt gatgcgtttt ttcatagcgc acctccgttc gaaccaccca
     4981 ggctggcggc gatcaggttt tctttggtca aagtcgcacc cgacaactcg gagcggatga
     5041 tgccgtcgtg gatcaccagc acccggtcgc acaggcccac cagctcttca tcatcgctgc
     5101 tataaaacaa aattgttttc ccttcgctgc ataactgtgt aagtagcgcg taaaactcag
     5161 ctttcgtgcc cacatccacg cctttggtcg ggtcgtccag caaaagaagc ttcggcttgc
     5221 gcatcagcca cttgccaatt acaactttct gtgcattccc acctgaaagg ctactgaccg
     5281 gttcccccag cccggccatt atcaagttca ggttagcgcc tgcctgttct gcatcttttt
     5341 tcgcttttcc aatgcgcagt gggaaaccat actgcgccca ggacggcaat tgcagatttt
     5401 ctagaatgga acggatgtag agcagacctt cccgggagcg atcaccgggg acgagcgcca
     5461 gcccaagctt catcgcctcg cgcgggtgcc ggagcctgca ctcattaccg aagagacaca
     5521 cttgcccaga gtagggcaca tcgccgaaca gcgagagcag caggtcacgc tgaccttgcc
     5581 cgcgcaaccc gccgatgccc accagttcgc cttcccgcac gctaaaactg acaccgcgca
     5641 gcgagtccgt ccgcaggtct tttacttcca ggcaaaccgg tttttcattg acctttgttt
     5701 cgccacgcgt aaaggtgaat accgactctt tctcaatcat caacttgact agttcttttt
     5761 cgttgatgtc ttccatattc ccagcgccaa cagttttacc gttgcgcaag acactgtatt
     5821 tgtcagcaat gcggaagatt tcctccatac ggtgcgagac aaacacaatt gccttaccta
     5881 cctctttcca attcctcacc agctcgaaga gacgctgcac ttggcggctg tccaagctag
     5941 cggttgcctc gtcgaggatg accatccacg ggttgaagct gattgctttg agaatttcga
     6001 tgatttgttt ttcgtccggg ggtagggaag caactgggtc gtctggccgc agatccgttt
     6061 tatatgttcc ccggaaaagg gataataatt gctctgtctg ctctatgatt gctttgcggt
     6121 taacggttcc tagaggcgtc atcggctcgc gcgtcaacca gatattttcg gcgacggtca
     6181 tgtgtgggat caggctcagt tcctggaaca cggtggcaat ccccatgttc ttggctgcct
     6241 gcggagaaga gaaatgcacg gttttgtcat cgaggagtag ttgccctcca tcgaggacaa
     6301 ctactccatt gatcacctta ctcattgtgc ttttgccact tccgtttgca cctatcaacg
     6361 ctacgacttc cccggtttga acatccaggt tgccatctga gagtgctacg acaccgccgt
     6421 agcgtttgac aatattacgg gcagaaagcg acttagccat gaagatttat ttgaaatact
     6481 gctggacttc ttcgtcggtc atgatgtcgt cgagcaggta agcatctggc tggccttcgc
     6541 acattgccag accttcatcc aggttctcgg atgtgatcac agccgccagc ggaatgacaa
     6601 acgatagacc attcacgccg cccagcttgc tctcatcaac cttcttgccc tgcagcatgt
     6661 tgacggcaat gcgcagaccg gtcggggata caccggggtg gttggcaaca gcaatggagt
     6721 cgaagtccgg attcatcgtc agggcttcct gccagagctt caaatactgg cagcggcctt
     6781 cacccacacc ctgcggccat tgagccgggt tggcagccat gacagcctgc aatgcaccga
     6841 tcgccatgcc gtcctgtgtc cagtagccat tcaggttcgg ataggcagcc aggaagttgg
     6901 acatcacctg ctggccggtg gcttcgtccc accgacctgt atcggtcgcc agaacgttga
     6961 tgccaggata gttggcaaag acttcggcta caccttccat gcgagccacg ttggccgggt
     7021 gtccggggaa gccttcgatc tggacgatat cgccttcgcc gcccagttta tcagctaacc
     7081 aacgagcgct ggtcatcgcc caggccttct ggtcaatgcc cgcattgtac acgccgggca
     7141 cgttcagttc ctggtccacc gagatgacga tgataccttt cgacagcgct tccaacaacg
     7201 tcgcgttcaa accattcaca tcgccgggat tgacgaggat ggcgtccacg tccttggcaa
     7261 tcaagttctg caactgctgg atctggcctg ccacgtccgt gtcggcgctt tcaatgacca
     7321 actcagtggt gatgccttga tccatatatt cctggttgac ttcgatcagc gaagcaatca
     7381 tctgcgtgcg gtattcgctg ctgatgaatg ggttcgaaac gccgattgtg aagggttctg
     7441 cagcgccttc ctgaacaaag aattgctgga cttcttcgtc agtcatgatg tcgtcgagca
     7501 ggtaagcatc tggctggcct tcgcacattg ccagaccttc atccaggttc tcggatgtga
     7561 tcacagccgc cagcggaatg ac
//
LOCUS       DBNHOH010000679         3595 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_233255, whole genome shotgun sequence.
ACCESSION   DBNHOH010000679 DBNHOH010000000
VERSION     DBNHOH010000679.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_233255"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="AC16V7_11795"
     CDS             complement(<1..767)
                     /locus_tag="AC16V7_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257846.1"
                     /translation="MKFSYLVSLPERILRASAASLGGFIYEASLLVLPGWLRETRLYT
                     AIVAGLLRIVIELVGGARGILPPKEISAQELATRKAAGTGIELAGIFTLGFSPLWIFA
                     AIADLTGGTRSYLRLLVSELKNDGLLTQEESINTVEELLDTLENTTGIAAEALDVPPL
                     NVSDMRRTWKDLRGNAASLPDADRLAALFAGMQQVAKQEKTSIHSLSTSIGAATVRAG
                     IQTGHIHILDFYIASLRTITAEGLTKFTQRVTLPYRMV"
     gene            complement(1075..2817)
                     /locus_tag="AC16V7_11800"
     CDS             complement(1075..2817)
                     /locus_tag="AC16V7_11800"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR6257847.1"
                     /translation="MQDASDQIQDLVALNQIIETLNRTVDMQSALDNTLARLLEVMGM
                     ETGWIFLRDPASQNLWAGRGYALASHKNLPPALALDNPEAWEKGCDCEGFCDKGKLVG
                     AYNEVQCSRLVEASGERGGLLVHASSPLRSGEEVLGILNIAAADWSSFTPRLLAFLTN
                     VGAHMGAALERARLYNLLRERRIDEQRTLISFTNQLLKRLELDELLNYLALEVPRLLE
                     AEGCAIVLHAHQPGELPTVFLHQWPEPNIPAMSAQEVLKYWQAQAPCCLATAPLVVES
                     GELGWLMLHSPEDKQLNDDETRLLQLLANQVALAIEKSRLHQVELSRQRLEVELETGM
                     RLQRSLLPKRLPDLPGWEFAAYYQPAQSLGGDFYDFFTLPGDDQRLGMVIADVMDKGL
                     PAALFMTLCRSIFRTTSISDISPAESLQRANLLIRRDIGCDDEYTDDIGYFVTAVYAT
                     LEIDSGRVNFANAGHNRPLCLRANGRVDELSSQGIVLGLFNDIFLEESQITLAPGDSL
                     MFYTDGTTEAINEQGELFNTSRLMNLLEHSDGLSAKVILDRVVATVSDWLGTQPQSDD
                     LALFIIRRSPSNDI"
BASE COUNT          883 a          893 c          909 g          910 t
ORIGIN      
        1 accatgcgat aaggtaaagt tacccgctga gtgaatttcg tcaggccttc agcggtgatg
       61 gttcgcaaag atgcgatgta gaaatcgaga atatgtatgt gacccgtctg gatcccagct
      121 cttactgtgg cagcgccaat tgaagtggat aaagaatgaa tcgaggtctt ctcctgtttt
      181 gccacttgtt gcatgccggc aaataaagcg gctaggcgat cagcatccgg taagctagct
      241 gcgtttcccc tcagatcttt ccaagttcgc cgcatatcgc taacattcaa cggcgggaca
      301 tccagcgctt ccgcagctat tcctgttgta ttctccaggg tatctagtaa ctcttcgacg
      361 gtattgatgc tctcctcttg agtcaacagt ccatcattct tgagctctga taccaacaat
      421 cgtagatagg atcgagttcc gccagtgaga tcggctatgg cagcgaatat ccacagaggt
      481 gaaaatccca gggtaaatat cccggcgagc tcgatgccag tgccggcagc tttgcgtgta
      541 gccagctctt gggcgctgat ttccttcgga ggcaggatac cccgggctcc gccgaccagc
      601 tcgatgacaa tacgtaacaa tccggcaacg atggcagtgt aaaggcgcgt ttcacgaagc
      661 catcctggca gcaccagcaa gctcgcttca tagatgaatc caccgaggct agcggctgag
      721 gctcgcagga tacgttcagg caatgaaacc aggtaagaaa atttcatgcg agtggaggca
      781 agtcctcccg gatccatgtt cttggtggtt tttcaaattt tgccatcttt gcattttcct
      841 ctctgacaat ttacataacg caaagaacgc ctcttgccat gagaggttat taagatcgtt
      901 ggcattgaga gtggtcgatg cagtaattgt cacagcacgc aaccgggtaa ctatcttgga
      961 catagatgga cttgctgttc agatctttcc aggtatcacc taacaagcga aaaagcgcca
     1021 ggaacagatt ttgaatacga tgcaatggcg attgaaacga gttttgccca acatttatat
     1081 atcattggat ggcgaacgtc gtatgataaa tagggccaga tcgtcggatt ggggctgcgt
     1141 gccaagccaa tcggatacgg tggcgactac tcggtccagg atcaccttag cgctgagtcc
     1201 atcactgtgc tcaagtaggt tcatcaagcg tgaggtgttg aaaagctcgc cttgttcatt
     1261 gatcgcttcg gttgtaccat cggtatagaa cataaggcta tcgccgggtg ctagggtgat
     1321 ctggctctcc tctaaaaaga tatcgttgaa aagtcctagg acgatgcctt gactggagag
     1381 ctcatctacc cgaccattgg ctcgtaggca aagcggtcga ttgtgcccgg cattagcaaa
     1441 attcacccta ccggagtcga tctcaagggt agcatatact gcggtgacga aatagcctat
     1501 atcgtccgtg tattcatcgt cgcagccaat atcacgacgg atcagcaggt tggcgcgctg
     1561 tagtgattca gcaggagata tatcactgat ggaggtggtg cgaaagatcg agcggcagag
     1621 ggtcatgaac aacgccgccg gaagaccctt atccatcaca tcggcgatca ccatccccag
     1681 tcgttgatcg tcacccggca gagtgaaaaa atcgtaaaaa tcacccccca aagattgcgc
     1741 tggctggtaa taagcagcga attcccagcc aggcaagtcc ggcaggcgct ttggcagcag
     1801 actgcgttgc aagcgcattc cggtttcaag ctccacttct aagcgctggc gggagagctc
     1861 tacctggtgc aggcgggatt tctcgatcgc cagggcgacc tggttggcaa gaagctgaag
     1921 taagcgggtt tcgtcgtcgt tgagctgctt atcctctggg ctgtggagca taagccagcc
     1981 tagctcgccg ctttcaacca ccagcggcgc ggtcgccaaa caacatggag cttgggcctg
     2041 ccagtacttg agcacctcct gggctgacat cgccgggatg ttgggttcag gccattgatg
     2101 caagaagacg gttgggagtt ctcccggctg atgagcgtgc aaaacaatgg cacatccctc
     2161 ggcctccagc aagcgtggca cttccaatgc cagataattg agcagttcgt ccagctccaa
     2221 ccgtttcaga agttggttgg taaaactgat cagtgtgcgt tgctcgtcaa tacgacgctc
     2281 acgcaatagg ttgtacagtc gggcgcgctc cagagccgct cccatgtggg cgcccacatt
     2341 agtcaagaaa gcgagtaggc gaggagtgaa agatgaccaa tcggcagcag cgatgttcaa
     2401 gatgccgagc acctcttcac cggagcgaag tggggaagag gcgtgcacca aaaggccacc
     2461 gcgctcgcca ctcgcttcaa ccaaccgact acattggact tcgttataag ccccaaccag
     2521 cttgcctttg tcgcagaatc cctcgcaatc acagcccttc tcccaggctt ccgggttatc
     2581 caaagccagt gcgggtggca ggttcttgtg agacgccaat gcataacccc gtccggccca
     2641 caagttttgg ctggctgggt cgcgcaggaa aatccagccg gtctccatgc ccatcacttc
     2701 cagcaggcgc gccagggtgt tatccaatgc actctgcata tccacggtgc gattgagcgt
     2761 ttcgatgatc tggttgagtg cgaccagatc ctggatttga tctgaggcat cctgcatggt
     2821 ttaccctccg ccttaggtag ctaagatctt gtagtaacgc agtacagtat ggaattgcct
     2881 atcgtttatt acttatcgca atctatcgca gtttcttcct tttccgattc ctgactaact
     2941 gtatgtgtac ttatgctcac agggaagcag attgaaaatt ctaaaaatcc atccattgtg
     3001 tgtgcatgat cttctttaat aatatagaca tacttaaaga ataactctcc agagatgcaa
     3061 gatactgctg taatcttcat acattatcgg caagtttccc tttacgtcat cagttatata
     3121 attttcattg acctcagaaa cggcagaata ttggtttaaa gaatttttca ttgcccacct
     3181 gcactatttc tattcatatg acccttaact tgagctttgt catgttgatt cccccctccc
     3241 tacacaattt atgtggggaa gtggattcta ttcttattgt tgtagatcca tgatgtcgag
     3301 tggcggttaa cattttttgg gattcttgtg ggggtaggtt gaaatgggct tcgaattgga
     3361 ttcctcaata attcactctg atctgctacg ctgcattgaa aactgttttg cctgatgata
     3421 attgttgata ttagtataca gacattctct tctcgattca atagggataa gtgaggttca
     3481 gacgaaagat cggacattgc cgtagatgag cgaagcgaaa gccctggcag caaccaaagc
     3541 cacttggtcc aattctggct gggagatacg caatttctgt ttgagcagca caaat
//
LOCUS       DBNHOH010000680         1094 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_40938, whole genome shotgun sequence.
ACCESSION   DBNHOH010000680 DBNHOH010000000
VERSION     DBNHOH010000680.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_40938"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="AC16V7_11805"
     CDS             <1..215
                     /locus_tag="AC16V7_11805"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6257848.1"
                     /translation="GLPVQFKVRHGNPVHEILEEVKSGGYDLIGMGSPHSAHSLRHLY
                     MPNVTAEVAEVTDCPVLSVRKGFSLV"
     gene            389..>1094
                     /locus_tag="AC16V7_11810"
     CDS             389..>1094
                     /locus_tag="AC16V7_11810"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIR6257849.1"
                     /translation="MKRRFPVPSDIEIAQEAVLKPITQIADEVGLLAEEIELHGTYKA
                     KVKLSVLERLKDVPDGIYVDVTAITPTPLGEGKSTTMVGLSQALGAHLGKRVFTCIRQ
                     PSQGPTFGIKGGAAGGGYSQVVPMEDFNLHLTGDIHAITAANNLLAAAIDTRIYHETT
                     QKDDLKLFNALCPPDKEGNRSFSLSMLHRLKKLGIDKTDPDQLTDEEKSRFARLDIDP
                     DSITWRRVVDTNDRFLR"
BASE COUNT          307 a          281 c          256 g          250 t
ORIGIN      
        1 cagggttgcc ggtccaattt aaagttcgac atggcaatcc agtacacgaa attttagaag
       61 aagtgaaatc aggaggatat gacctgatag ggatgggctc cccacatagc gcacatagtt
      121 tacgccattt atatatgccc aatgtgaccg ctgaggtcgc tgaagttacg gattgtccag
      181 tgttgtctgt acgaaagggt ttttcactag tctgaacacc aaattgtgac actcggcata
      241 aacgaaaatg atatgcgtct cttgattaga ttaatgaaac ctgtcacaat agatacgtta
      301 ataaaaatca tcaaaatacc tatacaaagg agagtgtatg tctgctgcta aagatttccc
      361 tggtctacgt cgcttcacac cgaacaaaat gaagcggcgc tttcccgtcc cttccgatat
      421 tgagatcgcc caagaagccg tcttaaaacc catcacccaa attgccgacg aggtcggctt
      481 attagcagaa gaaattgagc tgcatggcac gtataaggcc aaagtaaagc tctcagtgct
      541 tgaacgcctc aaagatgtcc ctgatggcat ctacgtggat gtgacagcga tcacacctac
      601 accactcggt gaaggaaaaa gcacgacaat ggtgggttta agccaggctc tgggcgcaca
      661 cctaggcaaa agagtgttca cctgcattcg ccaacccagc cagggtccaa cctttggcat
      721 taaaggcggc gccgctggtg gtggatacag ccaggtggtc ccaatggagg acttcaacct
      781 gcacctcacc ggcgatatcc atgccattac tgctgccaac aatctactgg cagccgccat
      841 tgacacgcgc atataccatg agaccaccca aaaggatgat ctcaagcttt tcaacgcctt
      901 gtgcccgcca gacaaggagg gtaatcgcag tttctctctc tccatgttac accgcttgaa
      961 gaaacttggc attgacaaga ccgatcctga tcaattaacc gatgaggaga agagccgctt
     1021 tgcccgcctg gatatcgacc ccgatagcat cacttggcgg cgggtagttg ataccaacga
     1081 tcgcttcctg cgtg
//
LOCUS       DBNHOH010000681         3051 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_361411, whole genome shotgun sequence.
ACCESSION   DBNHOH010000681 DBNHOH010000000
VERSION     DBNHOH010000681.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_361411"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1447
                     /locus_tag="AC16V7_11815"
     CDS             <1..1447
                     /locus_tag="AC16V7_11815"
                     /inference="COORDINATES: protein motif:HMM:NF025011.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HIR6257850.1"
                     /translation="GRLPWPMFTRRYGTIREMGAGRRDRERPYQTPTSPAETLWYRTL
                     VARAFLDTPDGPQEFAYIPGDIFRLLPDPQGELTAPLGRPGTPSERAHTILANDRILD
                     HACTLLAALRLGLHLDSVEFTRASWAFTSPFAPSPTALLAMLNATDLLDAGTNLPQPE
                     ATRQFLEYPRGKALALLARAWHHNPAFNEFRMLPDLSFEGEWQNDPLRARYAIIDLLS
                     KVPHNTWWSLPGFVSAVKHHQPDFQRPAGDYDSWFVRHKVSGEYLRGFEHWDEVDGAL
                     VRYIICGPLHWLGVLDLAAPAPGAQPAAFRFSRWSVELLSGAPAQGLEVEDDSFLISS
                     DARLRAPRLVPRAARYLVTRFCAWEGANEDGYLYRITSDSLSRARDQGLRAGHLLSLL
                     RRHALTVPPSLTRALERWEEHGSEARLERALILRLRTPETLQLLRNSRYARYLGDPLG
                     PTTIIVNPGAGEKVLAALAEMGYLGEMEGRE"
     gene            1629..>3051
                     /gene="pdxR"
                     /locus_tag="AC16V7_11820"
     CDS             1629..>3051
                     /gene="pdxR"
                     /locus_tag="AC16V7_11820"
                     /inference="COORDINATES: protein motif:HMM:NF052282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070279 - vitamin B6 binding [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal 5'-phosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:2000142 - regulation of DNA-templated
                     transcription initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MocR-like pyridoxine biosynthesis transcription
                     factor PdxR"
                     /protein_id="HIR6257851.1"
                     /translation="MRIPIDRESATPIYKQIIQFLRDEIESGALLAETRLPSTRSLAE
                     SLGVNRITVTNAYAELEAEGLIYGKTGSGFYVAELMLPQMRMNTGPATSTDWPLWQQS
                     LLSRGWTPVMGESERLAHTVESPDLISFSQGISDFRLIPADDIRKAIQAVLRRDGVDA
                     MGYGDRTGYPPLRETISHILASQGVPTHPDSILITSGSQQAVALVAHLLLRPGDVVLV
                     ESPSYIGVIDLFRSMDVRLLGIPIDEQGMRMDALEDALRNVNPKLIYTIPTFNNPTGV
                     CLSSQRRRQMIALADRYNTPILEDDFAGDLRYEGRAQPALKALDPGGLVIYVSTFSKM
                     LAPGLRLGFLAADGPVYERLVACKQAYDLATSNLIQRALEAYITVGRYQAHLRRACQV
                     YGSRRDAMISALSKYLPAEASWNKPQGGLFVWLKLPDELSTNELYPLAGEEKVSFAPG
                     SLFYPGARHQPYMRLNFVAHPVDV"
BASE COUNT          650 a          912 c          823 g          666 t
ORIGIN      
        1 gggcaggcta ccctggccaa tgttcacccg ccgctatggg acgattcgcg agatgggcgc
       61 tggtcgtcgc gaccgggagc gaccctatca aactcctact tcaccagccg aaaccctctg
      121 gtaccggacc ctggtggcgc gcgccttcct ggacactcct gacggtcccc aagagttcgc
      181 ctatatccct ggagacatct ttaggctact ccctgacccc caaggcgaat tgaccgctcc
      241 actgggaaga ccaggcacgc catccgagcg cgctcacacg atcctggcta acgaccgcat
      301 actggatcat gcttgcacgc tattggcagc cttgcgcctt gggctgcatc tggactcagt
      361 cgagttcacc cgggcatcct gggcattcac ttcaccattt gcccccagtc caaccgccct
      421 gctcgccatg ctaaacgcaa cggacttgct ggatgctggt accaacttgc ctcaaccaga
      481 ggcgacccgc cagttcttag aatacccgcg agggaaagcg ttagccctac tggcgcgtgc
      541 ctggcaccac aacccggctt tcaacgagtt ccgcatgtta cctgacttaa gtttcgaggg
      601 ggaatggcaa aatgacccct tgcgcgcccg ctatgcaatc attgacttac tctctaaggt
      661 gcctcacaac acatggtgga gcttgcctgg cttcgtttct gcggtcaagc accaccagcc
      721 tgatttccaa cgccctgccg gagattacga ctcatggttt gttcgccaca aagtcagcgg
      781 tgaatatctg cgcggttttg agcactggga tgaagtggat ggcgcgctag tccgttatat
      841 catctgcgga ccattgcact ggttaggcgt tctagatctg gccgcgccag cccccggagc
      901 acagccagct gcattccgct tctctcgctg gtctgtagaa ctgctcagcg gagcgcccgc
      961 tcaaggcttg gaagtagaag acgattcttt cttgatcagc tcggatgccc gcctgcgcgc
     1021 tcctcgcctt gtgcctcgag cggctcgata cctggtgacc cgcttctgtg cctgggaagg
     1081 cgctaacgaa gatggatatt tgtaccgcat cacttccgat tctctatccc gcgctcgcga
     1141 ccagggtttg cgtgcagggc acttgctcag tctgcttcgc cgccatgcct tgaccgtgcc
     1201 tcctagcctg acaagagccc tcgagcgctg ggaggaacat ggcagcgaag cgcgcctgga
     1261 gcgcgctctc atcctgcggc tgcggactcc ggagactctc caattactca gaaattcgcg
     1321 ctatgcccgt tacctggggg atccgctggg gccaacaaca ataattgtca atcctggggc
     1381 tggagagaaa gtgcttgccg ccctggctga gatgggctat ttaggggaga tggaaggtcg
     1441 ggagtaggtt gagaattagg ggggtaatcg gccctattta agacaatgta gtcaggttta
     1501 gatatggggg ataattcacg cggcctggta tagattggca ccaattggat ctatataatt
     1561 tcccaatttc gatgtagtat acatatacca gatgaccaat tggatcgaat agatttaagg
     1621 agctaggtat gcgtattcct atcgatcgtg aaagcgctac gcccatatat aagcagatca
     1681 tccagttttt acgagatgaa atcgagtccg gggcactgct agccgagacg cgcctgccct
     1741 ccacccgcag cctggcagag agcttgggcg taaatcgcat cactgtgacc aacgcttacg
     1801 ccgaattgga ggccgagggg ctaatctacg gtaaaacagg aagcggtttt tacgtcgctg
     1861 agcttatgct gccacaaatg cgcatgaaca caggtcctgc aacaagtaca gattggcctt
     1921 tatggcaaca gtccctgttg agccgaggct ggacacccgt catgggtgag agcgaaagac
     1981 ttgcccacac cgttgaaagc ccagacctga tctccttctc gcaggggatc agcgacttcc
     2041 ggctgattcc agccgacgat attcgcaaag cgatccaagc cgtattgcgc cgggatggcg
     2101 tagacgctat ggggtacggt gatcgcactg gttaccctcc cctgcgcgaa accatctcac
     2161 acattttggc cagccaaggt gtgcccaccc acccagatag catcctcatc acctcaggct
     2221 ctcaacaagc ggtggcactt gtcgctcacc tgctactgcg tccaggagat gtggttttag
     2281 tcgaaagccc atcctatatc ggcgtgatcg acctgttccg ctcgatggat gtccgtttgc
     2341 tgggcatccc tatcgacgaa caaggcatgc gcatggatgc gttagaagat gccctgcgca
     2401 atgtgaatcc caagctgatt tataccattc ccacttttaa taacccgacc ggagtctgcc
     2461 tgagcagcca gcggcgcaga cagatgattg ccctggctga tcgctacaac acacctatcc
     2521 tggaagacga ttttgcgggt gacctgcgct acgaaggccg agcccagcca gcgctcaaag
     2581 cgctggaccc aggcgggctg gtcatctatg tcagcacgtt ctccaagatg ctcgcaccag
     2641 gtctgcggct gggtttcctg gcagccgatg gcccggttta cgaacgactg gtagcctgca
     2701 agcaagccta tgacctggcg acatccaacc tgatccagcg cgctttggaa gcttacatca
     2761 ccgtcggccg ctatcaggcc cacttgcgcc gcgcctgcca ggtttacgga agtcggcggg
     2821 acgctatgat atcggcccta agcaagtatc tgcctgcaga ggcaagctgg aacaagccac
     2881 aaggcggctt gttcgtgtgg ctcaaactcc ccgacgaact gtccaccaac gagctgtatc
     2941 cgctagccgg ggaagaaaag gtctcatttg cgcccggatc cctcttctat cccggggcgc
     3001 gccaccagcc ttatatgcga ttaaactttg tcgcccatcc ggtagatgta a
//
LOCUS       DBNHOH010000682         2593 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1323244, whole genome shotgun sequence.
ACCESSION   DBNHOH010000682 DBNHOH010000000
VERSION     DBNHOH010000682.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1323244"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..469)
                     /locus_tag="AC16V7_11825"
     CDS             complement(92..469)
                     /locus_tag="AC16V7_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257852.1"
                     /translation="MKDYAIELEIFEGNGGQPRTDGTHPDFAREGICAWMYGGLGETS
                     YQVGQKFRYPEDLGELCPWLVDSLTGLIRALEHGGTLSWKYQGTPYEKVIDPDGVTTE
                     YVRCLDPTASGIVIKVTRTALPD"
     gene            complement(495..1178)
                     /locus_tag="AC16V7_11830"
     CDS             complement(495..1178)
                     /locus_tag="AC16V7_11830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016107.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR6257853.1"
                     /translation="MDKKLIARFAIRETMGVVIMGVALFWSAGRIDWWPAWALLAVML
                     AWIIAMGFAIIRFNPDLLAERLGPRKGGKQWDMTIMGIVGLAELARLIIAGLDQRYGW
                     TGGFPIAAQIAALAVCALGYALGVWATASNAFFSQIVRIQTERGHAVATGGPYQYVRH
                     PAYVGTILYQLAMPVLLASWWALIPGGLNTILLVIRTALEDRTLQAELSGYADYARLV
                     RHRLLPGIW"
     gene            complement(1329..1781)
                     /locus_tag="AC16V7_11835"
     CDS             complement(1329..1781)
                     /locus_tag="AC16V7_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257854.1"
                     /translation="MSPERLESEAEWEVWYQDTFDQESARQVEVVGQGLVEGLIELWA
                     RHLFETVQASGQQGFSRFNLWLKQERRSIEVVGEWDGQVRLRGWVFAGERQANTGYAE
                     KGDKALLNLIAASHKDLILRGETSEAILATAIASGGREDFVERLLSMK"
     gene            complement(1926..2504)
                     /locus_tag="AC16V7_11840"
     CDS             complement(1926..2504)
                     /locus_tag="AC16V7_11840"
                     /inference="COORDINATES: protein motif:HMM:NF019844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR6257855.1"
                     /translation="MESGFFFPLIGVLLSLLWVYFSFRYYLYYRGLIYSRDSNQEKSV
                     RLMGRAQLSLLVSGLVFIAMLVTLFGLSGTAISPPAAATPAPGEGADQLLPTPSPVME
                     LVLTPSPVSSPTPEVASSSGFARIGNTNTFGVYVRSEPGLKFEIVTQLSDGNRVELMG
                     EAQLADGFNWQQVRLEDGRVGWVADNFLIPEP"
BASE COUNT          635 a          766 c          633 g          559 t
ORIGIN      
        1 tggagttagg catgatcggc atccaaaacg ctgtgccttt ttgagaaggt tctaggacgc
       61 ctggatggtg gcaacgcaat tctcttgatc atcaatctgg caaagcggtt ctggtcacct
      121 tgatgacgat accagatgct gtggggtcaa gacagcgcac atactcggtt gtcaccccgt
      181 caggatcgat gactttttca tatggagtgc cctgatactt ccaggacagc gtcccaccat
      241 gttcgagcgc gcggattaac cctgtcaagc tatccaccag ccaaggacac aattccccaa
      301 gatcttcagg ataacgaaat ttctgtccca cctgataact cgtctcgcca agtcctccat
      361 acatccaggc acaaatgcct tccctcgcga agtctggatg cgtcccatca gtgcgtggct
      421 gcccgccatt gccctcgaaa atttctaatt cgatagcata atctttcata tattctcctt
      481 gtcgttgggt gagactacca gatgcctggc aacaggcgat gccgcaccag gcgagcgtag
      541 tcggcataac cacttagctc agcctgcaag gtgcggtctt caagggctgt gcggatcaca
      601 agaaggattg tgttcaatcc gcctgggatc aatgcccacc aagaagccag cagcactggc
      661 atggccagtt gatacagaat tgtgccgacg taagctggat ggcgcacata ttggtagggg
      721 ccgcctgtgg caacagcgtg accacgctcg gtctgtatgc ggacaatctg cgagaagaag
      781 gcgttggagg cggtagccca aacgcccagg gcatatccca gggcgcaaac agccagcgca
      841 gctatttgag cagctattgg gaaaccgcca gtccagccat aacgctgatc aagtccagcg
      901 atgatcaacc gcgccagttc cgcgagaccc acaatgccca tgatggtcat gtcccattgt
      961 ttgcccccct tgcgaggtcc caggcgctca gccagcaggt ctggattgaa acgtatgatg
     1021 gcgaatccca tggctatgat ccaagccagc atcacagcca gcagtgccca ggcaggccac
     1081 cagtcaatcc ggccagccga ccagaacaga gccacaccca tgatgacaac ccccatggtc
     1141 tcacggatgg caaaacgtgc gataagtttt ttgtccatgt cttttcactt cctaatcggg
     1201 ttacttatag agaatagacg gtgtgtctac aagccaacag catcatgaag ctcctggcta
     1261 aaacaatcaa agggggcagc tgttcgtaga gcagggtgcg ggcaggtcag gctcatattc
     1321 aaagcagact atttcatact caacaaccgc tccacaaaat cctcacggcc cccggaagct
     1381 attgccgttg ccagaatcgc ctcgctcgtc tcgcctctca agataagatc cttgtgcgat
     1441 gccgcgatca ggttcaataa cgccttatcg cctttctcgg cgtatcctgt gtttgcctgt
     1501 ctctcgcctg caaataccca gccccgcaat cgcacctgcc catcccactc acccacaact
     1561 tctatgcttc gcctctcctg tttcaaccac aaattgaacc ggctgaaccc ttgctgcccg
     1621 cttgcctgga cggtctcaaa caagtgacga gcccaaagct cgattaaccc ttccaccaac
     1681 ccttgcccaa caacctccac ctggcgggca ctctcctgat caaaggtatc ttgataccag
     1741 acctcccact ccgcctcaga ctctaatctc tcaggactca tttacattcc ttgtgccttc
     1801 tgcgcaaaca ggtgaatagc gctcctctca catcttaact tagtatatat caatttgcat
     1861 ggcttcagca ccagcggttc aagatcaaca ttgtgcggtg gctttaaact gcgcggaagc
     1921 tcggttcaag gctcgggaat taagaaatta tctgcgaccc agcccacgcg accgtcctcc
     1981 aggcgcactt gctgccagtt aaacccatcg gcaagctgcg cctcgcccat cagctcaacc
     2041 cggttgccat cggatagttg ggtaacgatc tcgaatttca gtccaggctc ggagcgcacg
     2101 tacacgccaa aagtgttggt gttcccgatg cgggcgaagc ccgagctaga agcgacttca
     2161 ggcgtcggcg agcttaccgg cgaaggggtg agcacaagct ccatgacggg gctgggagta
     2221 ggcaataact gatccgcgcc ttcacccggc gctggtgtgg cggcggcagg gggcgatatg
     2281 gcagtccccg ataagccgaa cagggtgacc aacatagcga taaaaaccag gcccgagacc
     2341 agcaaggata gctgtgccct gcccatcagg cgcacagatt tttcctggtt gctgtccctg
     2401 ctatagatca ggccgcggta ataaagatag taacggaagc tgaagtagac ccacagcagg
     2461 ctgagcagca caccgatcag aggaaaaaag aagcccgact ccattagtat agtattttag
     2521 gctatttttt accctgcgca aaaaacctta atcgatatat ttggggaaaa aagaatcgtc
     2581 gtctaacctg ccc
//
LOCUS       DBNHOH010000683         2517 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1323326, whole genome shotgun sequence.
ACCESSION   DBNHOH010000683 DBNHOH010000000
VERSION     DBNHOH010000683.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1323326"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="AC16V7_11845"
     CDS             <1..845
                     /locus_tag="AC16V7_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257856.1"
                     /translation="VRYGGVFHISKMEVEKMRIQLPLLVLIVILSLNACTLPISLPGD
                     IQTAIIKTESSNPTSTTNPEHGNPPTEELTRTLEPTLATSLPPTVQPSPTPTPDLRVI
                     IGKPEDYILQKEDLPDKYILKPGWSTPHNNSEILSARGVVEGKAYLEATGRIGGWIVY
                     YDLVESTTIAPEVIESYLVMYDNVYGSKIAKSPEWDTLEENQEIIDIDLVLGEWNRVT
                     IMRERQSNGKYYVWYAIEFIYRNIWTEVMAGGMESDVQHDYVENVARVVLAKLETAPL
                     GEPK"
     gene            complement(927..2036)
                     /locus_tag="AC16V7_11850"
     CDS             complement(927..2036)
                     /locus_tag="AC16V7_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR6257857.1"
                     /translation="MTDIRVQKFAQILIDHSTQVKPGDRVAITATTAAEPAVRALYEL
                     VLERGGYPHALLDFAGENEAYYAHASDDQLDFVPLFHKMAFEEFDVLIKLRSETNTRA
                     LSNVDAARLTRRQKAMFSLIATQMRRGADGTLRWMSTLYPTPAYAMEAEMGFNEYQDF
                     YFRACHADEGTPDPVAYWLGVKREQQRIIDWITGHDQVTLRGPNVDLRLSVKGRTFVN
                     CYGYNNLPDGEIFTGPVEDTAEGWVRYTYPAVHQGRLVEGIELTFEKGRVVKASAQKN
                     EDLLLKMIDSDAGARYIGEFAIGTNYGINRFSHNSLLDEKIGGSFHMALGAGYPESGS
                     QNKSSIHWDMICDLRQDSEIRVDGELMYRNGQFVI"
     gene            2155..>2517
                     /locus_tag="AC16V7_11855"
     CDS             2155..>2517
                     /locus_tag="AC16V7_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257858.1"
                     /translation="MQLNTSIEGYLKSNLSAYLDILHQMVEINSFTANPAGVNRLGEL
                     TAKVFSSLGFQAEYVQSINPQFGRHLILTRQGKTLESGGAAFPVGMVSHLDTVYSPEE
                     EQLHGFTWRPEGKRIYGPG"
BASE COUNT          617 a          626 c          678 g          596 t
ORIGIN      
        1 tcgttaggta tggtggcgta ttccatatat ctaagatgga ggtggaaaaa atgagaatac
       61 agttaccgct tcttgtgctg atcgtaattt tgtctctcaa cgcgtgtacg ttgcctatat
      121 cattgcccgg ggacatccag acagcgataa ttaaaaccga atcctccaat ccaacatcta
      181 ccactaatcc agaacatggt aatccgccca ctgaagaact aactcgtact ttagaaccca
      241 cccttgcgac ctctttgcca ccaaccgtac aaccatctcc gaccccaaca cctgacttgc
      301 gtgtgattat cggtaagcca gaggactata ttttgcagaa ggaagatttg ccagataaat
      361 atatcctaaa gcctggatgg tcgacccccc acaataattc ggagatattg agcgccaggg
      421 gcgttgtaga gggaaaggct tacctggaag caacaggacg aattgggggt tggattgttt
      481 actatgattt agttgaaagc acgacgattg cacctgaggt gattgagtcg tatcttgtta
      541 tgtatgacaa tgtttatggc tcgaaaatcg ctaaatcacc ggagtgggat acgcttgaag
      601 aaaatcagga gatcattgat attgatttgg ttctagggga atggaatcga gtgacaatca
      661 tgcgtgaaag acaatccaac ggcaaatatt atgtatggta tgcgatcgaa ttcatctatc
      721 gcaacatatg gacggaagtc atggcggggg ggatggaatc tgacgtccaa catgattatg
      781 tcgaaaacgt tgccagggta gttcttgcaa agctcgaaac tgcaccgttg ggtgaaccca
      841 aatgaagtca acccagacgc attcgtcacg tcggagaatg tgcacccagt gaggcgagga
      901 ttatggcggg cctagggtct cactggtcat atcacaaact gcccatttct atacatcagc
      961 tcgccatcca cgcggatctc tgaatcctgc cgcaggtcgc agatcatatc ccaatggatg
     1021 gagctcttat tttggctgcc gctttccggg tagccggcgc ccagcgccat gtggaaggag
     1081 ccaccgatct tttcgtccag caggctgtta tgggagaagc ggttgatacc gtaattggtg
     1141 ccaatggcga actccccgat atagcgagca ccggcatcac tgtcgatcat cttcagcaag
     1201 agatcctcgt tcttctgggc gctagccttg accactcttc ctttttcgaa cgtcagctcg
     1261 atgccttcaa cgaggcgacc ttgatgcacg gcaggatagg tgtagcgtac ccacccttcg
     1321 gcggtgtcct ccaccggccc ggtaaagatc tcgccatcgg gcaggttatt atagccatag
     1381 caattcacaa atgtgcgtcc cttgacagag agcctgagat ccacatttgg gccgcgcaac
     1441 gttacctggt cgtgaccggt gatccaatca atgatgcgct gctgctcacg cttaacgccc
     1501 agccagtagg caaccgggtc tggcgtgccc tcatcggcgt ggcaggcgcg aaagtaaaaa
     1561 tcctggtatt cattgaaccc catctcggct tccatggcgt aggctggggt ggggtagagc
     1621 gtgctcatcc agcgcagagt gccgtcagca ccgcggcgca tttgagtggc aatcaaggaa
     1681 aacatggctt tctgcctgcg cgtcaggcgg gcggcatcta cgttagagag ggcgcgtgtg
     1741 ttggtctcag agcgcagctt gatgagcaca tcgaattctt caaaagccat cttgtggaaa
     1801 agcggcacga agtcaagctg atcgtcgctg gcgtgggcgt agtaggcttc gttttctccg
     1861 gcgaagtcca gcagcgcgtg ggggtaaccc cctcgctcaa gcaccagttc gtacaatgct
     1921 cgcacagcgg gctcagccgc ggtggtagcg gtaatagcta cccggtcacc aggttttacc
     1981 tgggtggaat gatcgatcag gatttgggca aatttctgta cgcgaatatc ggtcaattct
     2041 gcgctcctct cgccagatag gaatgggggg attataacat ccgcaagata ctttaccctg
     2101 aggttataat tggatcatag tcccggaggt tactcaggag gattggcaag cattatgcag
     2161 cttaacacga gtattgaagg ctaccttaag agcaatctat ccgcctatct ggacatattg
     2221 caccagatgg tggagatcaa tagcttcacc gcaaaccccg ctggagtaaa ccggctgggc
     2281 gagctgaccg cgaaggtctt ttcgtctctc ggtttccaag cggaatatgt gcaatcgatc
     2341 aacccgcagt ttggcaggca tcttatcctg actcgtcagg ggaaaaccct ggaaagtggg
     2401 ggagcggcat ttccggtagg gatggtctct cacctggata cggtttacag cccagaggaa
     2461 gagcaattac acggtttcac ctggcgtcca gagggcaagc gtatctatgg gcctgga
//
LOCUS       DBNHOH010000684         5933 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_683066, whole genome shotgun sequence.
ACCESSION   DBNHOH010000684 DBNHOH010000000
VERSION     DBNHOH010000684.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5933)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_683066"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="AC16V7_11860"
     CDS             complement(<1..639)
                     /locus_tag="AC16V7_11860"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR6257859.1"
                     /translation="MLLNDRYRLDSELGSGGMGVVYRAQDMLLERPVAVKVLNQGGLG
                     SEGHSRLLQEAQAAAKLNHPNIVAIYDAGEVDSLAYIVMELVEGASLYEQRPKTIDEI
                     IPIASQLCAALEHAHSHGIIHRDLKPENVMVTSDGIAKLMDFGLARSVASRLTMEGTI
                     IGTVFYLAPEQALGKELDGRADLYALGAMLYEFCTGQLPFSGDDPFAIITQHL"
     gene            complement(918..2552)
                     /gene="groL"
                     /locus_tag="AC16V7_11865"
     CDS             complement(918..2552)
                     /gene="groL"
                     /locus_tag="AC16V7_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561045.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR6257860.1"
                     /translation="MAAKQLVFSEEARRRLQRGMDLLATAVVTTLGPKGRNVAIDRKF
                     GSPTITHDGVTVAKEIELEDPFENMGAQLLKEAATKTNDIAGDGTTTSTVLAHSIVTD
                     GMKSLAAGFNPMLLKRGVEAATKAVADQIKAQSIPIENKEDIANVASISAQDRAIGDL
                     IAEVMDKVGKDGVITVEESKGLEFETEYVEGMQFDRGYISPYFITDPEHMEASISDAY
                     LLIHDKKISAAQDIVPVLEKLVQVGKRELVIISEDVDGEALATLVLNKLRGMLNVVAV
                     KAPGFGDRRKAMLGDIATLTGATVISEELGRKLESATMQDLGRAEKIVVTKDDTTIVG
                     GKGDTSQIKGRIEQIRVEIDKSTSDYDREKLQERLAKLSGGVAIIRVGAATETELKEK
                     KHRVEDALSATRAAVEEGIVPGGGVALINAMAALEGVKMDNEDAQVGVNIVRKALEVP
                     MRKIAENAGKDGAVILDTVRRTQKEKKNKKIGYDVLTEEYVDMVKSGVIDPAKVTRGA
                     LENATSIAAMILTTEALITDVPEKEKAPAAPPMPEY"
     gene            complement(2592..2888)
                     /gene="groES"
                     /locus_tag="AC16V7_11870"
     CDS             complement(2592..2888)
                     /gene="groES"
                     /locus_tag="AC16V7_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR6257861.1"
                     /translation="MSMSLKPLGNRVVVEPIEMEEMTTGGIVLPETAKEKPQKGKILS
                     IGPGERDEKGTRVPMDVKAGDVVLYAKYAGTEIKVDGKKLLILKESDLLAIVES"
     gene            complement(3044..4483)
                     /locus_tag="AC16V7_11875"
     CDS             complement(3044..4483)
                     /locus_tag="AC16V7_11875"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6257862.1"
                     /translation="MARQLTGKRPNFRPVKSGSNFTRVMVLLGLILAGLWLLQGVQRG
                     QVEPLFLATPTPTRIASSYIFEAQAYFQAGKLDDPDPPKPAPPNPDAIDTYRKALEVD
                     PTNAQAWAEMARIQTYSINMLSNDAERRARLADALESINKAVELNPDDSTIHAVRAFV
                     LDWNASYATTEEGVAEFLKEAEGEAVRAYQLDPLNALALSFYAEVLLDQQKWIQAEQY
                     AAQATALDPNSMDAHRVYATVLESLGQYNSAIKEYLLASSINPNLTFLYVYVGRNYLR
                     LRLYEQALEYFAKAADINTQLGVQNPIPNLEIAKAYSQQGEFFIAARNAEKALSYDPT
                     NANTYGQLGIIYIRSRNYEGALSALRCAVRGCSAEENEIAMNLVDQELLPNSVAVDGL
                     PMTNLAVAYYYVEYGTVLAFLSRPEQNNCPEAIQVLDGVSAQFSDDPVLMEIVADSQG
                     ICARLGSSGAPPAPPDEMMSDIPTPVVSP"
     gene            complement(4549..5808)
                     /locus_tag="AC16V7_11880"
     CDS             complement(4549..5808)
                     /locus_tag="AC16V7_11880"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6257863.1"
                     /translation="MYLRGPKWSMARRLKKRPSPWRILLLLSFIVIALYFNQVIVPAT
                     PPLFIPTSTPTRSPESVVNEAEQLFEEGKLAQAIAAYQQAVIADPSNPILYVTLARIQ
                     IFTGEYQDALTNAQNALLKNPNNPMAHAVQAYAQSFLGDHLAAEASIKRSLELDPNSA
                     LAHAYYAEILMNKGDFEDFEKAAAESRLAKELDPSSLESHRARGLVLLYTGTENLDEA
                     ITELKAAVAVNDKIADLHLLLGFAYRLKGENDQAVDELLAAYALNPKDPNIPTEITLA
                     YSNEGQFGKAAQYAEDAVKIDPSDAKLRGNLGVMYYKNNEMDKAIQELALAVRGGTTV
                     EGTPVEGLPLDYGKVAEYYWFYGFALAKRNRCAEAVPVFQALVSGVPDYDLAVENAIA
                     GMDLCKASLGTPEVESGESTPEVTATP"
BASE COUNT         1237 a         1605 c         1677 g         1414 t
ORIGIN      
        1 caggtgctga gtgataatgg caaaggggtc atcaccggag aagggaagtt gaccggtgca
       61 aaattcatac agcatggcac ccaaagcata caggtcggca cggccatcca gttcttttcc
      121 aagcgcctgc tcaggggcca ggtagaagac cgtgccgatg atggtgcctt ccattgtgag
      181 gcgcgaggca accgagcggg ccaagccgaa atccatcagc ttggcgatgc catccgaggt
      241 gaccatcacg ttttcaggct tcagatcgcg gtggataatg ccatggctat gggcgtgctc
      301 aagcgcagcg cacagctggc tggcgatcgg gatgatttca tcgatcgtct tgggtctttg
      361 ctcatacaac gatgccccct caaccaattc catgacgatg taggctaggc tatcgacttc
      421 tccagcatcg taaatcgcta cgatattggg atggttaagc tttgccgccg cctgggcttc
      481 ttgcagcaac cgcgagtgtc cttcgctgcc caaccctccc tggttcagaa ctttgacggc
      541 gacgggccgt tcgagcagca tatcttgggc gcgatagacc acccccatcc cgccactacc
      601 gagctcgcta tccaaacggt agcgatcgtt caataacatc cccatagcta tcatggcgat
      661 tactctaccc ttctctggat agacaggttt tccgcagccg aacccaacat gcgacgcctg
      721 cccatctata taaatttcag gacaaacccg ctaaactttc gcttatatgt ctaatataat
      781 tttattatac atgccttcgc gttttatttg gcataaaatg aacgggagag ggtgagcaat
      841 caccatatct acccgcgtga gacaaaaaaa tccccccatc ctgtatggac ggggggagca
      901 ggtgctttga gtgaatttta atattccggc atagggggag cagccggggc tttctctttc
      961 tcgggcacgt cggtgatcaa ggcctcggtg gtcaggatca tggcagcgat ggaggtagcg
     1021 ttttctaggg cgccgcgggt cactttggcg ggatcgatca cgccgctctt caccatgtcc
     1081 acgtactcct cagtgagcac gtcgtagccg atcttcttgt tcttcttttc cttctgcgtt
     1141 cgtcgcacgg tatccaggat aacggcgcca tctttgccag cgttctcggc gatcttgcgc
     1201 atgggcactt ccaaggcttt acggacgata ttcacaccca cctgggcatc ctcattgtcc
     1261 atcttgacgc cttccagggc agccatggcg ttgatcaggg ctacgccacc gcccggcacg
     1321 atgccctctt ccacggcggc gcgggtggcc gaaagggcgt cttcgacgcg gtgcttcttc
     1381 tctttcagct cagtttcagt ggccgcgccc acgcggatga tggcgacgcc gccggagagc
     1441 ttggccaggc gctcctggag cttctcgcgg tcatagtcgc tggtgctctt atcgatctca
     1501 acgcggatct gctcaatgcg gcctttgatc tggctagtgt cgcccttgcc accgacgata
     1561 gtggtgtcgt ccttggtaac cacgatcttc tcggcgcggc ccaggtcttg cattgtggcg
     1621 ctctccagct tgcgaccgag ctcctcggag atcacggtag cgccggtcag ggtggcgata
     1681 tcgcccagca tagccttgcg gcggtcgcca aagccggggg ccttgacagc gaccacgttg
     1741 agcatgccac gcagcttgtt caacaccaag gttgccagag cttcaccgtc cacgtcttcg
     1801 gagatgataa ccagctcgcg cttgcctacc tgcaccagtt tctccagcac ggggacgatg
     1861 tcttgggcgg cggagatctt cttgtcgtga atcagcaggt aagcgtccga gatggaggct
     1921 tccatgtgct cggggtcagt gatgaagtaa ggcgagatat agccgcggtc gaactgcata
     1981 ccttcgacat actcggtctc aaactccaaa cccttggatt cttcgacggt gatgacgccg
     2041 tctttgccga ccttatccat cacctcagcg atcagatcgc cgatagcgcg gtcctgagcg
     2101 gagatggaag ccacgttggc aatatcttct ttgttttcga ttggaatgga ctgggccttg
     2161 atttggtcag ccacagcctt ggtagcggct tctacgccgc gcttgagcag catagggttg
     2221 aaaccagctg ccaggctctt cataccatcg gtgacgatgg agtgcgccag gacggtggag
     2281 gtagtggtac cgtcgccggc aatatcgttg gttttggtag cggcttcttt gagcagctgg
     2341 gcgcccatgt tctcaaaggg gtcttccagc tcaatttctt tggcgacagt gacgccgtcg
     2401 tgggtaatgg tgggggaacc gaacttgcgg tcaattgcga cgttgcggcc tttcgggccg
     2461 agggtggtga ccaccgcggt cgccagcaag tccatgccgc gctgcaggcg gcggcgggct
     2521 tcttctgaga ataccagttg tttagcggcc atagaacatt tactccttat ctaggatgaa
     2581 ttcagggggg gttacgattc aacgattgct aacagatcgc tctctttgag gataagcagc
     2641 ttcttgccgt ccaccttgat ctcggtgccg gcatatttgg cgtacaggac aacgtcgcca
     2701 gccttgacat ccatggggac gcgtgtgcct ttctcatcgc gctcgccagg tccgatagac
     2761 aggattttgc ctttttgcgg cttttctttt gcggtttcgg ggagcacgat accgccagtg
     2821 gtcatctctt ccatctcgat gggctcgacc accacgcggt ttcctaaagg cttgagtgac
     2881 atagacatga ctacctcctt ggggtataag gtttgaattt caagtgacat gcagaagagt
     2941 tagcactcag tgtgcgagag tgctaactcg cggtcaaatg atacccattt tttcgatggg
     3001 agtcaagagg gagcttgaaa ttttaataaa attctgacat tccctacgga gatacgaccg
     3061 gtgtaggtat gtcgctcatc atctcatccg gaggtgcagg cggcgcgcca ctcgatccca
     3121 ggcgtgcgca gataccctgg ctgtctgcca caatctccat cagtaccggg tcatccgaga
     3181 actgagcgct cacaccgtcc aacacctgta ttgcctcagg acagttgttc tgctctggtc
     3241 ggcttaggaa tgccaagaca gtaccatatt caacatagta gtaagccacg gccaggttgg
     3301 tcatcggcag gccatccaca gcgacgctat tcggaagcag ctcctgatcc accaggttca
     3361 tagcgatctc gttctcctcg gcgctgcaac cacgcacagc acagcgcaac gcgctcaagg
     3421 cgccttcata gttgcgcgac ctgatgtaga tgatgcctaa ttgcccgtag gtattagcat
     3481 tggtgggatc gtagctaagt gctttttcag cattgcgggc ggcgataaag aattcacctt
     3541 gctgggagta agccttggcg atctccaggt tggggatggg gttttgcaca cccagctggg
     3601 tgttaatatc cgcggctttg gcaaagtatt ccaaagcctg ctcgtagagt cgcaggcgca
     3661 ggtagttgcg accgacgtag acgtacagga aggtcaggtt gggattaatg gatgaagcca
     3721 gcagatattc tttgatggca ctgttgtact gcccaagcga ctcgagtaca gtggcataaa
     3781 cacggtgagc gtccatcgag ttggggtcca gggcggtggc ttgtgcggcg tactgctcgg
     3841 cttgtatcca cttctgctgg tcaagcagca cctccgcata aaatgacagc gcaagggcat
     3901 tgagtggatc cagctggtag gcgcgcacgg cttccccttc agcctccttc aagaactcgg
     3961 ctaccccctc ctccgtagta gcgtaggacg cattccaatc caggacaaag gcgcgcacag
     4021 catggatggt gctatcatcc gggttcagct cgacagcctt attgatcgat tcaagggcat
     4081 ctgccaggcg ggcgcgccgt tcggcgtcgt tgctgagcat attgatggaa taggtctgga
     4141 tgcgggccat ctcggcccag gcctgggcgt tggttggatc gacctccagg gctttccgat
     4201 aagtatcgat ggcatccggg ttgggcggag cgggctttgg agggtccggg tcatccagct
     4261 ttcccgcctg gaagtatgcc tgagcctcga atatatagga cgaagcgatg cgggtggggg
     4321 ttggcgtggc taagaagaga ggctccacct gtccacgctg cacaccctgt aataaccaca
     4381 ggccagccag gataaggcct aataaaacca tcacacgggt aaagttagat cccgatttga
     4441 ctggacggaa atttgggcgc ttaccggtga gttgtcttgc catcgtattg aatcattcaa
     4501 attcgcgcag ttggggattc atttccatca actgcgtaaa tcctaacatt aaggggttgc
     4561 cgtgacctcg ggtgtgctct cgccagattc tacttcagga gttcccaggc tggctttgca
     4621 taaatccata ccagcgatgg cattctcaac cgccaggtca taatcgggca cgccagagac
     4681 cagcgcctgg aagacaggaa ctgcttcagc gcaacggttt ctcttcgcca gggcaaaacc
     4741 gtagaaccag taatattcag ctaccttgcc gtagtccagg ggcaggcctt ccacaggcgt
     4801 cccctcaaca gtagtcccgc cccgcactgc caaagctaat tcttgaatgg ctttgtccat
     4861 ctcgttattt ttgtaataca tgacgcctaa gttgccgcgc agtttggcat cagaggggtc
     4921 aatcttgacc gcatcctcgg catattgggc agccttgccg aactggccct cattggagta
     4981 tgcgagcgtg atctcggtag ggatattggg gtctttcggg ttgagggcat aagccgccag
     5041 tagctcatcc actgcctggt cattttcacc cttgagacga taggcaaatc ccagcagcag
     5101 gtgcaggtcg gcgatcttat cattaacggc aacagcagcc tttaattcgg tgatggcctc
     5161 atcgaggttc tcggtcccgg tgtagagcaa caccaaacca cgggcgcgat gcgactccag
     5221 ggaactgggg tccagctcct ttgccagccg ggattcggct gccgctttct cgaaatcctc
     5281 gaagtcgcct ttgttcatca ggatctcggc gtaataggcg tgagccagcg cgctgtttgg
     5341 gtcaagctcc agggatcttt tgatggatgc ctccgcagcc aggtgatcgc ccagaaagct
     5401 ctgcgcatag gcctgaacag cgtgcgccat ggggttattg gggtttttaa gcagggcgtt
     5461 ttgggcattg gtcagggcgt cttggtattc tccagtaaag atctggatac gggccagggt
     5521 gacatacaag atggggttgc tcggatcggc gatgacagct tgctggtaag ccgcaatcgc
     5581 ctgtgccagc ttgccctcct caaagagctg ctcggcttcg ttgacaaccg actcgggact
     5641 gcgtgtgggc gtggaggtag ggatgaacag aggtggcgtg gcagggacaa tcacctggtt
     5701 gaagtacagg gcaataacga taaagcttaa taggagcaag atgcgccagg gactagggcg
     5761 cttcttcagg cggcgggcca tgctccattt tggaccacgc aaatacatat tttgatcata
     5821 ccattggcaa ggaatagacg caagaaaggt ttttattggc tctttgaaaa ttccttgcca
     5881 accgatcacg aaatagcata gaattacact atgcgctttg acctatttac cct
//
LOCUS       DBNHOH010000685         2498 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1067886, whole genome shotgun sequence.
ACCESSION   DBNHOH010000685 DBNHOH010000000
VERSION     DBNHOH010000685.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1067886"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="AC16V7_11885"
     CDS             <1..565
                     /locus_tag="AC16V7_11885"
                     /inference="COORDINATES: protein motif:HMM:NF024799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR6257864.1"
                     /translation="FWGAEDYVQGYYSGVRMGDEGATKGVCINHQVGQTGLDARCRGF
                     VDALAEKGIPAEVLGVKGEDAAQVQTTISDYYTANPDVNAFLNLGPAGSDPFYAFVEA
                     EGIQPGQIIHGTFDISPAGVKAIKDGVTLFILDAQPYLIGYGTVESLVNYLRYNITPA
                     TGISATGPGFIDKSNVDIVEALAGTYR"
     gene            651..1655
                     /locus_tag="AC16V7_11890"
     CDS             651..1655
                     /locus_tag="AC16V7_11890"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6257865.1"
                     /translation="MIVEDTWRKRLAHSFSMSTVLGPLVVLIIIFLLFSIFVPNFLTL
                     RSITGIINAITLTGTLTVGVAMLMISGEFDLSVGSIMAVGAYIYGGLTMAHQPALGFI
                     LALVIPALLGMVNGLILVWTGIPSFIVTLGTKYLFRGLLWVVTAGTMLQTIDRLPIYN
                     LFNGRLDIVNNLFTGANFRSSLLWLLMLVIFFQFILVRTRFGNHVFAVGGNQGAALGQ
                     GVNVKFVKLMGFIITGALAGFTGVLLFSQYYTVRVASGDGLELSAIAAAVVGGTLITG
                     GAGSIWGALVGALIISMLRTGVVLMNLPYIPADNFEAIIGVTIIAAAILNKYLRGQSS
                     "
     gene            1687..2457
                     /locus_tag="AC16V7_11895"
     CDS             1687..2457
                     /locus_tag="AC16V7_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6257866.1"
                     /translation="MVDKKHVHGKNSLVWMEKISKKFGTITALQDVDFGVEGGEVRAL
                     LGDNGAGKSTLIKVLMGVYPPTTGQIYFQGQPVRISSPREAREVGIEAVYQDLALVNL
                     MSISRNFFLGREMVRNIGPIRWLDVRKMNEICRKSLHDIGIAIRSPAERVGSLSGGER
                     QSIAIGRGIHFGAKVLILDEPTSALSVAETRKVLTYIANAKERGLGVIFITHNVHHVY
                     MVADSYTVIRHGRNVGTYFKGELTEDDIADLITGDREV"
BASE COUNT          545 a          625 c          688 g          640 t
ORIGIN      
        1 cttctggggc gcagaggact acgtccaggg ttattatagt ggtgtccgca tgggggatga
       61 gggtgccaca aaaggcgtct gcattaacca ccaggttggt caaactggtc ttgacgctcg
      121 ttgcagaggc ttcgtcgatg cgctggctga gaaaggcata ccggccgaag tgctaggtgt
      181 gaaaggcgaa gatgcagccc aagttcaaac caccatcagc gactactata ccgccaaccc
      241 ggatgtgaac gccttcttga atctgggacc tgccggttcc gacccgttct atgcctttgt
      301 cgaagctgaa ggcatccagc ccggtcagat catccacggc accttcgata tcagccctgc
      361 cggtgtcaag gcgatcaagg atggtgttac cctgttcatc ctggatgccc agccgtacct
      421 gatcggctat gggactgtcg aatctctggt gaactacctg cgctataaca tcacgcctgc
      481 cacgggcatc agtgccactg gccccggctt tatcgacaaa agcaatgtcg atattgtcga
      541 agcactggct ggcacctaca ggtaaatatc gttaaaacct cagagcgggc agggctgaaa
      601 gtcctgcccg ctcacccaag ctctgtcggt atgcggaggg cgagaagaaa atgattgtcg
      661 aagatacctg gagaaaaaga ctcgctcatt cattctctat gtcaaccgtc ctaggccctc
      721 tggtggtctt aattatcatc tttttgcttt tttctatttt cgtccccaat ttcctaacct
      781 tacgctcgat taccggtatt atcaacgcca ttacgctcac tggaacactc actgtgggtg
      841 ttgcgatgtt gatgatctcg ggcgagtttg acctttcggt tggctcgatc atggcagtgg
      901 gagcctatat ctacggcggg ctcaccatgg cacaccagcc tgctcttgga tttatcctgg
      961 cgttggttat ccctgcgctc ttgggcatgg tgaacgggtt aatccttgtt tggacaggta
     1021 tcccctcttt tatcgtaacg ctggggacaa aatatctgtt ccgcggattg ttgtgggtgg
     1081 tcactgccgg gaccatgctg cagaccattg atcggctgcc aatctataac ttattcaatg
     1141 gtcggttaga tattgtaaac aaccttttta ccggtgcgaa ttttagatct tctctgctgt
     1201 ggttgttgat gctggtgata ttcttccaat ttatcctggt gcgtacccgc ttcggtaatc
     1261 atgtctttgc ggttggcggg aatcagggcg ctgctctggg tcagggggtt aatgtcaaat
     1321 ttgtcaaatt gatgggcttt atcatcaccg gcgctctggc gggcttcacg ggagtccttc
     1381 ttttcagcca gtactacaca gtgcgagtgg cttctggaga cgggctagag ctgagtgcta
     1441 tcgccgccgc agtcgtgggg ggcacgctta tcacaggtgg agctgggagt atatggggcg
     1501 cgctggtggg cgcgttgatt atcagcatgt tgcgcactgg tgtggtgttg atgaacctcc
     1561 cgtacatccc cgccgataac ttcgaagcca ttatcggtgt cacgatcatc gccgcggcaa
     1621 tcctaaataa atacctgcgg ggtcaatcat cttagcgacc ctggatcatc tcttcggagt
     1681 gtaaaaatgg tggataaaaa acatgtgcac ggtaagaatt ccctggtctg gatggaaaaa
     1741 atatccaaga aatttgggac cattactgcg ttgcaagatg tagactttgg cgtggagggc
     1801 ggcgaagttc gcgccttgct gggcgataac ggagctggga aatccaccct gatcaaagtc
     1861 ttgatgggtg tataccctcc caccacaggt cagatctatt ttcaaggcca gcccgtgagg
     1921 atctcctcgc cgcgtgaggc acgcgaggtc ggcattgaag cggtatatca ggacctggca
     1981 ttggttaacc tgatgagcat ctcgcgcaat ttcttcttgg gccgcgagat ggtaagaaac
     2041 atcggaccta tccgctggct ggatgtgcgc aagatgaacg aaatctgccg caaatctttg
     2101 catgacattg ggattgcaat tcgctcacct gctgaacgcg tcggcagctt gtctggcggc
     2161 gaacggcaat ccatcgccat cgggcgcggc atccatttcg gagccaaggt gctgatcctg
     2221 gatgagccga cctcagccct gtcggtggct gaaacccgca aagttttgac ctatatcgcc
     2281 aatgcaaagg agcgcggcct gggggtgatc tttatcacgc ataatgtgca ccatgtctac
     2341 atggtcgctg acagctatac tgtaatccgc cacggtcgca acgttggcac ctattttaaa
     2401 ggtgaattga cggaggacga catcgctgac ttgatcaccg gtgaccgtga agtatgattg
     2461 aaaaggaagg ttttgatggc tagcaattat aacggtcc
//
LOCUS       DBNHOH010000686         3294 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1387702, whole genome shotgun sequence.
ACCESSION   DBNHOH010000686 DBNHOH010000000
VERSION     DBNHOH010000686.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1387702"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="AC16V7_11900"
     CDS             <1..911
                     /locus_tag="AC16V7_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR6257867.1"
                     /translation="ACSSSASPTPQTEFAQPLSNRTDDVHKEGEVHRHINPEDATAQI
                     QLVLVPSELIVGPNRFAVGIFDQGGDLVQDAGVHLHFYDLTDSSNPSFESEADAYPIS
                     TPDGQVTIFAFEREFPRGGLWGVEVEVSFRDGSQAINGIRFSVASDSPSLGPGEKVPP
                     MHTPTLADAGQDLGLLSSAEAPNPAFYQLSLDQAMSSGKPTLLLFATPAFCQTRFCGP
                     SYEIFSHLEQRYRNTLNFIHVEVFTGLPNPEANNWAVAPSMEQFGLESEPWIFLIGQD
                     GTVLYRVEGVFTQEEIEGHLKAKLGL"
     gene            1050..2012
                     /locus_tag="AC16V7_11905"
     CDS             1050..2012
                     /locus_tag="AC16V7_11905"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR6257868.1"
                     /translation="MNPILSFGIQLIQALQTLSPALDVPMRFFTFLGTIEFYILIIPV
                     FYWLVDSQLGFRILLALLSTDILGTAFKHLLHQPRPYWVGDVKAMGDEISYGIPSTHA
                     SDSLTVWGFFAYQMKKNWLWAASAGLVFLISLSRLYLGVHFPQDVLGGWLIGAFVLYV
                     FIKLEPRVLSWLKNVSQLTQIGLAFGISVLVILVGLLVGLAIASTPDPAEWAQYATQA
                     RSPSHYYTLGGVFFGAASGFVLMWHRARFGTQGSGIQKAGRYLLGIAGVLVIYMGLDA
                     VFGLIAADETAAGYLLRYIRYASLAFWMTFGAPWAFLKLKLAHK"
     gene            2225..>3294
                     /locus_tag="AC16V7_11910"
     CDS             2225..>3294
                     /locus_tag="AC16V7_11910"
                     /inference="COORDINATES: protein motif:HMM:NF015649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR6257869.1"
                     /translation="MILRFYLLGPAIVTWKHTPLNITRRQVRKLLYLLATQESAVPLE
                     RLHYLFWSDKPESFCRNNLSHLLTHARRSLPNKDILTVTNSIAMLDPSQTWSDVGEFK
                     NLVKNEARIENLPSLVKAYDLYRGPYLDGVQLPGGRELEYVVEQERYTLERQYLNGLI
                     KLIEIETQRGKLENALEYAYKYLEIDNLSEEVHRQLIRLHAWMGNQDRAIRQYKLCEE
                     ILAREIQATPSKKTQAMYQEALSGNLSEYRVEKKPPEYKTSKVREIPISQRLSHLDQL
                     QKNFDNFFLGVGGVVLLSGEIGVGKNNLIQESLLRNQNNSLILHAHCDPGSESISFLP
                     LIDIATDVSNYQDKIRFLNPDST"
BASE COUNT          750 a          936 c          889 g          719 t
ORIGIN      
        1 cagcctgctc ctcatcggcc tcccccaccc cgcagaccga gtttgcgcag cccctctcca
       61 accgcacgga tgatgtgcac aaggagggcg aggtgcaccg gcatatcaac cccgaagacg
      121 ccacggcgca gatccagctg gtgctggtgc cgtccgagct gatcgtaggc cccaaccgct
      181 tcgcggtggg catcttcgac cagggcggcg acctggtcca ggatgcgggt gtgcacctgc
      241 acttctacga cctgaccgac tcttccaacc cttccttcga atccgaggcg gatgcctacc
      301 ccatcagcac cccagatggg caggtgacga tcttcgcttt cgaacgggag ttcccccgcg
      361 gcgggctgtg gggtgtggag gtggaggtca gcttccggga tggctcgcaa gccatcaacg
      421 gcatccgctt ctcggtcgcc tcagactccc cctcgctggg gccgggcgag aaagtcccgc
      481 ccatgcacac ccccacgctg gcggatgcgg gccaggacct gggcttgttg agctcagccg
      541 aggcgcccaa cccggccttc taccagctca gcctggacca ggccatgagc agcggcaagc
      601 ccaccctgct gctcttcgcc acgccggctt tctgccagac ccgcttctgc ggcccctcgt
      661 acgagatttt cagccacctc gagcagcgct accggaatac cttgaacttc atccatgtgg
      721 aagtgttcac cgggctgccc aatcctgagg ccaacaactg ggcggtggcg ccgtctatgg
      781 agcagtttgg actggagagc gaaccctgga tcttcctgat cgggcaggat ggcaccgtgc
      841 tctaccgggt ggagggggtg tttacccagg aggagatcga ggggcatctc aaggcaaaac
      901 tcgggctgta gcaagaccgg cggaagctgg ggcggaaata gccaggtggg gatcattaaa
      961 taaccggcgc caagcggcaa atcgaaagcc tgcgataggg gttatatctt tataggtcgc
     1021 aacgcggcaa cacgaggagg agacccacca tgaaccctat cctgagcttc ggcatccagc
     1081 tcatccaggc tttgcagacc ctgagcccgg ccctggacgt ccctatgagg tttttcacgt
     1141 tcttggggac gattgagttt tacatcttga tcataccggt gttttactgg ctggtcgaca
     1201 gccagctggg attccggatc ttgctggcgc tgctcagcac cgacatcctg ggcacggctt
     1261 ttaagcattt gctgcaccag ccgcgcccat actgggtggg tgatgtgaaa gccatgggcg
     1321 acgagatatc ctacggcatc ccctccacgc atgccagcga ttcgctgacc gtatggggat
     1381 ttttcgccta ccagatgaaa aagaactggc tctgggcagc ctccgcgggg ctggttttcc
     1441 tgatcagcct gtcgcgcctg tacctgggcg tccatttccc ccaggatgtg ctgggcgggt
     1501 ggctgattgg ggccttcgtg ctctatgtgt tcatcaaact cgagcccaga gtactgtcgt
     1561 ggctgaaaaa cgtcagccag ctgacccaga ttggtctggc cttcggcatc tcggtattgg
     1621 tgatcctggt cggcctgctg gttggcctgg caatcgcctc cacacccgac ccggcggagt
     1681 gggcgcaata cgccacgcaa gcacgctcgc cttcccatta ctacaccctg ggcggggtgt
     1741 tcttcggcgc agcctccggc ttcgtgctga tgtggcacag ggcccgcttt ggcacccagg
     1801 ggtcaggcat ccaaaaagcg gggcgctacc tgctggggat cgccggggtg ctggtgatct
     1861 acatgggcct ggatgcagtc ttcggcctga tcgccgccga tgaaaccgcc gccggctatc
     1921 tcttgcgcta tatccgctat gccagcctgg ccttttggat gaccttcggc gcgccctggg
     1981 cctttctgaa gctgaagctg gctcataaat aattttcccg aattcagact tacttacctg
     2041 tacacccccc cctgcgtaaa cataaacacc tttatgcggt gtaactttta gtgtttatat
     2101 acacagctga tacacaaaat tattgacaca gaacgcaatt acctgtatta tatatacaca
     2161 tatatagtta gttttactct aggcgagtat cagattaggt accaagaaac aaggatgaag
     2221 gccggtgata ttaaggttct atttgcttgg tcctgccatc gtcacctgga agcacacccc
     2281 tcttaacatc acacgtcggc aagttagaaa actcttatat ctgctcgcaa cacaggagtc
     2341 tgctgttccc ctcgaacggc tgcactattt gttttggagt gacaaacccg aatccttttg
     2401 ccggaacaat ctctcgcatc tcctcacgca cgcccggcgt tccctgccaa acaaagatat
     2461 cttaaccgtt acaaattcca tagccatgct tgatccaagc cagacatggt cggatgtggg
     2521 ggaatttaaa aacctggtaa aaaacgaggc gagaattgaa aacctgccgt cactggtcaa
     2581 agcctatgac ttgtataggg gtccatatct ggatggagtt caattgccag gcggccggga
     2641 gctggagtac gtggttgagc aggaacgata cacgctggag agacagtact taaatggctt
     2701 gatcaaactt atcgaaatag aaacacaaag ggggaaactg gagaacgccc tcgagtatgc
     2761 ttacaaatat cttgaaattg ataacctgtc tgaagaggtt caccgccaat tgatcaggtt
     2821 acatgcttgg atgggcaacc aggatcgcgc catcaggcag tacaagttat gcgaagagat
     2881 actcgcaaga gagatacagg caacaccatc caagaaaacg caggcaatgt accaggaggc
     2941 actaagcgga aacctgagcg aatatcgcgt tgaaaaaaaa ccacccgagt ataagaccag
     3001 caaagtgagg gaaattccga ttagccaaag gttatctcat ttagatcaac tacagaaaaa
     3061 ctttgacaat tttttcttag gcgttggtgg ggtggtgttg cttagcggcg agataggggt
     3121 gggtaaaaac aacctgatcc aggaaagcct gctccgcaac cagaacaact cgctcattct
     3181 acatgcgcac tgtgacccgg ggtctgagtc aatctcattt ctccccttaa tcgatattgc
     3241 aacagatgtt tccaattacc aggataaaat ccggttctta aaccccgatt caac
//
LOCUS       DBNHOH010000687         2046 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1195565, whole genome shotgun sequence.
ACCESSION   DBNHOH010000687 DBNHOH010000000
VERSION     DBNHOH010000687.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1195565"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="AC16V7_11915"
     CDS             complement(<1..982)
                     /locus_tag="AC16V7_11915"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015590050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0004473 - malate dehydrogenase
                     (decarboxylating) (NADP+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent malic enzyme"
                     /protein_id="HIR6257870.1"
                     /translation="MLKEKPSVNVDELLAKAKKPSADALKLHPFYRGKVETTLKCVVR
                     DFRDFAIWYTPGVAAPCREIAANPEKVYEHTNKWNTVAVVSDGTRVLGLGDIGPKAGL
                     PVMEGKALLYKYLGGVDGVPIMLDTKDPDAIINTVLLLQPGLGGVNLEDISQPKCFYI
                     LDTLREKAEIPIWHDDQQGTATVTLAGLMNALKVVGKKKEEARIAFIGCGAANVACSR
                     LIYGWGVDPANCIMVDSKGILGMHRKDLELRKAEFVDKWRLCQITNAEGRQGDIPAAM
                     KGMDVVIALSRSGPGVILPEWVSSMEKDAILFACANPIPEIWPWEAREAGA"
     gene            complement(993..1232)
                     /locus_tag="AC16V7_11920"
     CDS             complement(993..1232)
                     /locus_tag="AC16V7_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257871.1"
                     /translation="MSDHASDLDKCTEDGPQSALERKLIEEYLSEKGYHLSDLQALPE
                     REARRLMREACKYASLKLAEVESRAQLRKEIHYPS"
     gene            complement(1345..>2046)
                     /locus_tag="AC16V7_11925"
     CDS             complement(1345..>2046)
                     /locus_tag="AC16V7_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR6257872.1"
                     /translation="AYRLGAKLSFIDTMQYHPTGAAYPEQILGQLVTEKVRGLGAQVC
                     NVDGEQFVFPLETRDVEAAAFIRECVERRAGVITPTGQPAVWLDTPLIEMLRGPGTVA
                     RELPAMVRQFKRFDIDLPGEPILVYPTLHYQNGGIAIRADGSTAIAGLFVAGEAAGGI
                     HGRNRLMGNSLLDICVFGRRAGVSAALWAQGVQPGHPTLAHLRDWERARRDSGLDEAA
                     ASPMILPDYTVREHL"
BASE COUNT          454 a          635 c          508 g          449 t
ORIGIN      
        1 gcgccccagc ctctcgcgcc tcccagggcc aaatctcagg tattgggttg gcgcaggcaa
       61 acaggatcgc gtccttttcc atgctggaca cccactctgg taagatcaca cctggtccag
      121 agcgggagag ggcgatgacg acatccatac ccttcattgc cgcgggtata tcgccctggc
      181 gaccttctgc attggtgatc tggcaaagtc gccacttatc gacgaactca gctttgcgca
      241 gctccaggtc tttgcgatgc atgcccagga tgcccttgct atccaccata atacaattgg
      301 caggatcgac accccagcca tagatgaggc gagagcaggc tacatttgct gcaccgcacc
      361 caatgaaggc aatcctagcc tcttcttttt tcttccctac gaccttgaga gcattcatca
      421 ggcctgccag cgtaaccgtg gcagtgccct gctggtcatc gtgccagatg gggatttcag
      481 ctttctcccg cagcgtatcc aggatataga aacacttagg ctgagagata tcttccagat
      541 tcacgccacc caaaccgggc tgcaataaca acaccgtgtt aatgatggcg tcgggatctt
      601 tggtatccag catgatcgga acgccgtcca cgccgcccag gtatttatac agcagggctt
      661 tgccttccat cacaggcaga ccggcctttg ggccgatatc acccaatccc agcacgcgtg
      721 tgccatcgct gacgactgcc accgtgttcc atttgttggt atgctcatac actttctctg
      781 ggtttgcggc gatctcccgg caaggcgcag ctacccccgg ggtataccaa atcgcgaaat
      841 ctctaaaatc gcgcacgacg cacttgagcg tcgtttcgac cttcccccgg tagaacgggt
      901 gcagtttaag cgcgtccgcc gacggcttct tggctttggc aagcagctcg tcgacgttga
      961 cagatggttt ttctttcaac atgagacact ccttacgaag ggtaatgaat ttccttgcgt
     1021 agctgagcgc gcgattcgac ctctgcgagc ttcaaggagg cgtacttaca cgcctctcgc
     1081 attaaacgcc tcgcctccct ctccggtaga gcttggagat cgctcagatg ataacctttc
     1141 tcggacagat attcctcgat gagtttcctt tccaacgccg attgaggtcc atcttcggta
     1201 cacttatcga gatccgaagc atgatcagac atggttcacc tccatatgcc tggtttacta
     1261 atctaattta caaaaacagt aatcttccta cagataccgg ttttcacccc aggcggcaaa
     1321 gatacgacga agaaattact tggtttacaa atgctccctc acagtatagt ccggcaagat
     1381 catcggcgaa gcagcagcct catccagtcc gctatcacgg cgagcgcgct cccaatcccg
     1441 caggtgggcg agcgtgggat gcccaggctg gacaccctgc gcccacaggg cagcgctgac
     1501 gcccgcccgg cgcccaaaga cacagatgtc cagcagacta ttgcccatca ggcgattacg
     1561 cccatgaata cctccggctg cctccccagc cacaaacaaa cccgctatag ctgtgcttcc
     1621 gtccgcgcga atggcaatac ccccattctg gtagtgcaag gtgggataga ccaggatggg
     1681 ctcgcccggc agatcgatgt cgaagcgctt gaactgcctc accatagctg gcaactcgcg
     1741 tgctaccgta ccagggcctc gcagcatctc gattaaggga gtgtccagcc aaactgcggg
     1801 ctgaccggta ggcgttataa caccagccct acgctcaaca cactcacgga tgaaggcggc
     1861 agcctccaca tctcgcgttt cgagtggaaa gacgaactgc tccccgtcca cattacagac
     1921 ctgcgcacca aggccgcgca ccttttccgt caccagctgc cccaggattt gctctggata
     1981 ggctgctccg gtgggatggt actgcatggt atcgatgaaa gacaacttgg ctcccaggcg
     2041 atatgc
//
LOCUS       DBNHOH010000688         5644 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1323716, whole genome shotgun sequence.
ACCESSION   DBNHOH010000688 DBNHOH010000000
VERSION     DBNHOH010000688.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5644)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1323716"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="AC16V7_11930"
     CDS             complement(<1..263)
                     /locus_tag="AC16V7_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257873.1"
                     /translation="MTSIFVSILAIAIVGFVLFRKGGDQRVHEHRTASRLMIVGLIPL
                     GLQIAIFMLFGLGEMASGEFSGAGHLLPAVATLVLMLLSWKRP"
     gene            complement(403..708)
                     /locus_tag="AC16V7_11935"
     CDS             complement(403..708)
                     /locus_tag="AC16V7_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HIR6257874.1"
                     /translation="MKHPIYRVVEFEIVNSYTLRVIFDDGKEQVIDFRPVLIGELYGP
                     LRDKTLFKKVEIDPEVHTLVWPNGADFDPATLHDWPENVDSMQELAKRWAAKKVEAT"
     gene            complement(730..999)
                     /locus_tag="AC16V7_11940"
     CDS             complement(730..999)
                     /locus_tag="AC16V7_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HIR6257875.1"
                     /translation="MPEICRFFGIIIRMFAEPGAPHHRTHFHAYYQNHVAIFSIDKVE
                     LINGSLPSRERRLVEAWAELHQGELSENWERLQSGQLPYKIAPLR"
     gene            1202..1777
                     /locus_tag="AC16V7_11945"
     CDS             1202..1777
                     /locus_tag="AC16V7_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013156845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HIR6257876.1"
                     /translation="MDTPSTSEELLNFFKALADANRLKIVGLLAQQPTTVEGLAAALG
                     LGASTVSHHLSKLAKAGLVSARTDGHYYIYSLETDALQAMAERLLSADELPRLSDDAD
                     LDAYDRKVLANFLDAEGRIKAFPAQEKKFQVILQHVVQAFEPGQHYTEKQVNEILGRY
                     HKDTASLRRGLIDYRLMARQSGGGEYWRTEA"
     gene            1948..3363
                     /locus_tag="AC16V7_11950"
     CDS             1948..3363
                     /locus_tag="AC16V7_11950"
                     /EC_number="4.1.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050371 - tyrosine phenol-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine phenol-lyase"
                     /protein_id="HIR6257877.1"
                     /translation="MTDHFKTMGQQFGRRSWAEPWKIKMVEPLTMTDRPYRQSAMQEA
                     GYNTFLLRSEDVYIDLLTDSGTSAMSDRQWAGMMLGDEAYAGSRNFYHLEEAIQTHYG
                     YRYLVPTHQGRGAEHLLSQAMIKPGQFIPGNMYFTTTRLHQELAGGTFVDVIIDEAHD
                     PQNMHPFKGNVDLDKLQDLIERVGAGNIAYISLAGTVNMAGGQPVSMENVRLLRDLCD
                     RYGIRIYLDATRMVENALFIQEREEGYAGKSIAQILKEFCSYTDGAWMSAKKDNLVNI
                     GGWLAVNHNDLFEELRNMVVIYEGLHTYGGLAGRDMEAMAIGIVEAVQDDHVRSRVGQ
                     VRYLGQLLLDWNIPIVQPVGGHAIFLDARSFYPHLSQDLFPAQTLATELYLDSGIRSM
                     ERGVVSAGRDPETGDHRYPKLELTRLTIPRRVYTQAHMDVVAESVKAVYDARQQTRGL
                     KLVYEPKYLRFFQARFEPIED"
     gene            complement(3460..4521)
                     /gene="thrC"
                     /locus_tag="AC16V7_11955"
     CDS             complement(3460..4521)
                     /gene="thrC"
                     /locus_tag="AC16V7_11955"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003346144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR6257878.1"
                     /translation="MKTTNTGVLSRYGHLLDLPDHTQPVTLLEGDTPLIPVPRLAQEM
                     GGGFELYVKYEGLNPTGSFKDRGMTAAISEALGREAKAVICASTGNTAASAAAYAARA
                     GMRAVVLIPEGKVAAGKLAGAVAYGAQVVQVKGSFDDALSLVVAISEKHPIALVNSLN
                     PYRLEGQKTAAFEICDALGNAPDLLCLPVGNAGNITSYWMGFKQYHALKGCGLPHFLG
                     VQAAGAAPLVLGHPVDKPETVATAIRIGRPARGEQAMQAAEESGGRIVAASDDEILAM
                     QRRLAGEGIWVEPASAAGLAGLASLLRRGEITVAGQRVVAVCTGHGLKDPDIIARQMG
                     KPTILPPTLEALEAVIQMP"
     gene            complement(4561..>5644)
                     /locus_tag="AC16V7_11960"
     CDS             complement(4561..>5644)
                     /locus_tag="AC16V7_11960"
                     /inference="COORDINATES: protein motif:HMM:NF019403.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HIR6257879.1"
                     /translation="GRMLALDALRGLIMILMAIDHASLFIRRSHPFELWNIPLPHYDS
                     AAAFLTRFVTHPCAPGFFFLMGAGIILFAASRRQMGWSQLRIARHLVLRGLLLILLEQ
                     LVLDPILGGEVNFKDLQVFYGLGGVLVVCALLVGLNRYILMTIGVGIILATQVLPPAL
                     QDLGLYPAPLSLLLLVPGGSQGWFVLYPVFPWSGISLLGMAFGHELLRDRERGYRLAL
                     WMGGISLALFLVVRMAGGFGNLRPAAGTGWIDFLNVVKYPPSLVFTLLTLGINLILLY
                     LFTRAGNWLEKWGKPLLAFGKAPLFFFLMHWFLYAAAGLYFLQPGRLPPMYLAWALGL
                     LLLYPVCVVYGNFKRGTPPESVWRLF"
BASE COUNT         1265 a         1649 c         1611 g         1119 t
ORIGIN      
        1 ataggccgct tccaggaaag cagcatcagc accagggttg caaccgctgg cagaaggtgc
       61 ccggccccgc tgaactcgcc ggacgccatc tcgcctagac caaacaacat gaagatggca
      121 atctgcagtc ccagcggaat taggccgacg atcatgagtc ggcttgccgt tctatgctca
      181 tggacacgct ggtcgcctcc cttgcggaac aatacaaatc ccacaatggc aatggcaagg
      241 atgctgacaa agatggaagt catacgatcc tcctttgtgc actattcccc atcaaactgg
      301 agatgctggc agccaccctg gactgcccgc caggacaggc ggtgggttga ctgatttttt
      361 acttgtatga tatctgggag atgttttttg ggaaacgctt tcctatgtgg cttcaacttt
      421 ctttgctgcc caccgttttg ctaactcctg catactgtcc acattttcag gccaatcgtg
      481 cagtgtagca ggatcaaaat cggcaccgtt aggccagacc aatgtgtgaa cttctggatc
      541 aatctctact tttttgaata gtgttttatc tcgtaatggc ccatacaatt caccaataag
      601 gaccgggcga aaatcaatga cctgttcctt tccatcgtcg aatatcactc gcagggtgta
      661 tgagttgact atttcaaact ctaccacacg ataaatcgga tgcttcatta tctcttttct
      721 ccaattctcc tatcgtaacg gtgcaatttt ataaggcaac tgaccggatt gtaaacgctc
      781 ccagttttct gagagctccc cttgatgcaa ttcagcccag gcctccacca atcgtctctc
      841 ccgagatggt aagctgccat tgattagctc gactttatct atactgaaga tggccacatg
      901 gttttggtaa taagcatgga aatgagtacg gtgatgggga gcacctggct cagcaaacat
      961 gcgaataatg atgccaaaaa agcggcagat ctcgggcacg tatgtttcct tccatatcgt
     1021 agtatcctat attattttac atcaaagttg cctagaacgg ccgccaacgt cagacctata
     1081 ttttcggggc gtacgctctt cgttattaat gactgaaatc gcatgttcat ccttgacaag
     1141 cccaacgaaa tggataaaat catttcgaag atagtcgaaa tgttacgaag gagttcggat
     1201 tgtggacacc ccatcaacca gcgaagagct gctcaatttc tttaaagccc tggcagacgc
     1261 caaccggctg aagatcgtcg gattgctggc gcagcagccc accaccgtcg aagggctggc
     1321 tgcggcgctc ggcctgggcg cttccactgt ctcgcaccac ctgtccaagc tggctaaggc
     1381 tgggctggtg tctgcgcgca cggacgggca ctactacatc tactcgctag agaccgacgc
     1441 tctgcaagcc atggctgagc gtttgctgag tgctgatgag ctgccccgcc tatcggacga
     1501 cgccgaccta gacgcttacg accgcaaagt gctggcgaat ttcctggatg ccgaggggcg
     1561 catcaaggct tttcccgccc aggagaagaa attccaggtc atcctgcagc acgtggtaca
     1621 ggccttcgag cccgggcagc actacactga gaaacaggtg aacgagatcc tagggcgcta
     1681 ccataaagac accgcatccc tgcgccgcgg cctgatcgat taccgcctga tggcgcgcca
     1741 aagcggcggg ggggagtact ggcgcaccga ggcttgagca cgcctgacgg atgaaatgac
     1801 aaaacattgg aagggattta acactaagta cacaaagtgc acacgaaggt caccaaaaat
     1861 aattaaaacc tttgtgtccc ttcgtgaaac ctttgagccc tttgtgttaa taaaacagac
     1921 acagccattg ctgtaaggag aatttggatg acagatcact tcaaaaccat ggggcagcag
     1981 ttcggacgcc gatcctgggc cgagccctgg aagatcaaga tggtcgagcc attgaccatg
     2041 accgaccgcc cttaccgcca gagtgccatg caagaagccg gttataacac cttcctgctg
     2101 cgttctgagg atgtctatat cgacctgctg accgacagcg gcaccagcgc catgagcgac
     2161 cgccagtggg ctgggatgat gcttggcgac gaggcctacg ccggtagccg caacttctac
     2221 caccttgaag aggcaatcca gacccactac ggctaccgct acctggtgcc tactcaccag
     2281 ggccgcggcg ctgagcacct gctcagccag gccatgatca agcccgggca gtttatcccc
     2341 ggcaacatgt acttcaccac cacccgcctg caccaggaat tggctggcgg cacgtttgtg
     2401 gatgtgatca tcgatgaagc tcacgacccg cagaacatgc accccttcaa gggcaatgtg
     2461 gacctggaca agctacaaga cctgattgag cgggtcggcg cggggaatat agcctatatc
     2521 agcctggcgg ggacagtcaa tatggccggc gggcagccgg tgagcatgga gaacgtccgc
     2581 cttctgcgcg atctgtgcga ccgctatgga atccgcatct atttggatgc aacccgtatg
     2641 gtcgagaacg ccttgttcat ccaggagcgc gaggaaggtt atgctggcaa gagcatcgcc
     2701 caaatcctta aagagttctg ctcctacacc gacggagcct ggatgagtgc caagaaggac
     2761 aacctggtga acatcggggg ttggttggcg gtgaaccaca acgatctttt cgaggaattg
     2821 cgcaacatgg ttgtgatcta tgaagggctg cacacctacg gcggcctggc cgggcgcgat
     2881 atggaggcca tggccattgg catcgtcgag gcggtgcagg acgatcatgt gcgctctcgg
     2941 gtaggccagg tgcgctacct ggggcagcta ctgctggatt ggaatattcc cattgtgcag
     3001 cccgtcggcg ggcacgccat cttcctggat gcgcgcagct tctacccgca cctatcgcag
     3061 gatctctttc ccgcccaaac cctggccact gagctgtacc tggattcggg tatccgctca
     3121 atggagcgcg gcgtggtcag cgccgggcgg gatcctgaga ccggcgacca ccgctatccg
     3181 aagctggagc tcacccgctt gaccatcccg cgccgtgtgt acacccaggc gcacatggat
     3241 gtggtggccg agtcggtcaa agcggtttac gatgcccgcc agcagacccg cggtctcaag
     3301 ttggtctacg agcccaagta cctgcgtttt ttccaggcca ggtttgaacc gattgaggat
     3361 tgaatatttc tgattgctga ttgccagtac accgcaatcg caagaggacg gcacgcgcag
     3421 tcacaatgtg gctgcgcgct gtctttggtt agctgatggc tacggcattt ggatcacggc
     3481 ttctagcgct tcgagggtgg gcggcaaaat ggtcggcttg cccatctggc gggcgataat
     3541 atcggggtcc ttcagcccgt gacctgtgca caccgcgacc acgcgctgac ctgcgacagt
     3601 gatctccccg cggcggagca gactcgccag cccggccagc ccggcggcag aagccggttc
     3661 cacccagatg ccctcaccag ctaagcggcg ctgcatggcc aggatttcat cgtcactggc
     3721 cgccacgatg cgcccgcccg attcttcggc agcctgcatg gcctgctcgc cacgcgccgg
     3781 gcgtccgatg cggatggcag tggccaccgt ctcgggcttg tccaccgggt gacccagcac
     3841 caaaggggcg gctccggcag cctgcacgcc caggaagtgc ggcagcccac atcctttcaa
     3901 ggcgtggtat tgtttgaaac ccatccagta agaggtgatg ttgcctgcgt tgcccaccgg
     3961 caggcagagc aggtcgggcg cgtttcccag ggcgtcgcaa atctcgaagg cggcggtctt
     4021 ctgcccctcc aggcggtagg gattgagcga gttgaccagc gcaatggggt gcttctcgga
     4081 gatggccacg accaggctga gggcatcatc gaacgagcct ttcacttgca caacctgggc
     4141 gccgtaggct acggcaccgg ccagcttgcc agccgcgacc ttgccctcgg ggatgagcac
     4201 cacagcgcgc atcccggcgc gggcggcgta ggctgccgcc gaggcggcgg tattgcccgt
     4261 ggaggcgcag atcacggctt tcgcctcgcg ccctaaggct tcgctgatgg cggctgtcat
     4321 gccgcggtct ttgaacgagc cggtggggtt gagcccctcg tacttgacgt atagctcgaa
     4381 accgccgccc atctcctgcg ccaggcgagg cacagggatt agcggggtat ccccctccag
     4441 cagcgtgaca ggctgggtgt ggtcgggcag gtcgagcaag tgcccgtagc gcgagagaac
     4501 gcctgtattg gttgttttca tggctgagca cacaccgtac aatatgtgtt gatcgacatt
     4561 ttaaaacaac ctccatacag actctggggg ggtgcctcgt ttgaagttgc catacaccac
     4621 gcagaccggg tagagcagta acaggcctaa tgcccatgcc aggtacattg gcggcagcct
     4681 gcccggttga aggaagtata acccagctgc cgcgtacaag aaccaatgca tcaagaagaa
     4741 gaacagcggc gctttcccaa atgccagcag tggcttgccc cacttttcaa gccagttgcc
     4801 cgctctggtg aacaggtaca ataaaatcaa attgatgccc agggttagca gcgtgaagac
     4861 caggctgggc ggatacttga ccacattgag aaaatcgatc cacccggtcc cagctgccgg
     4921 tcggagattg ccgaaaccgc ccgccatgcg caccaccagg aagagggcaa ggctgatccc
     4981 gcccatccat agcgccaggc gatagcctcg ctcccggtcg cgcagcagct cgtgaccaaa
     5041 cgccatgccc agcagggaga tgccggacca ggggaataca gggtacagca cgaaccatcc
     5101 ctgggatccg ccaggcacga gcagtagaag gctgagcggc gcagggtaaa gccccaggtc
     5161 ttgtagtgcc ggggggagca cctgggtggc caggataatt ccaactccga tggtcatcaa
     5221 gatgtacctg ttaaggccca ccagcaaggc gcataccacc agcacgcccc ccagcccgta
     5281 gaacacctgg agatccttaa aattcacctc gccgcccaag atgggatcca gaacaagctg
     5341 ctccagcagg atgagcaaga gaccccgcag gacaaggtgt cgagcgatgc ggagctgcga
     5401 ccatcccatc tggcggcggg atgcggcgaa caggatgatc ccggccccca tcaagaagaa
     5461 gaaccccggc gcgcacgggt gggttacgaa gcgcgtcagg aaggcggctg cgctgtcgta
     5521 atggggcagc gggatattcc acagctcaaa cgggtgtgat ctgcggatga agaggctggc
     5581 atggtcgata gccatcagga tcatgatcaa gccgcgcagc gcatccagtg ccagcatccg
     5641 cccc
//
LOCUS       DBNHOH010000689         3145 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1003773, whole genome shotgun sequence.
ACCESSION   DBNHOH010000689 DBNHOH010000000
VERSION     DBNHOH010000689.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1003773"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="AC16V7_11965"
     CDS             complement(<1..551)
                     /locus_tag="AC16V7_11965"
                     /inference="COORDINATES: protein motif:HMM:NF014662.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR6257880.1"
                     /translation="MPEHPGGLQAPVMITEHLVLINSLAEQGEPFATAVVVRAEKPTS
                     AKPGAKALITADGTLKGWVGGSCAEPSVKKEALRALADGKPRLLRLCPPESLGTGAQE
                     GVVEIALTCISGGTYEIFIEPQLVQPRLVVVGHLATAESLVKLGRAMGYPVSVAGYEV
                     SLERFPGAMHLQDQLDFSALQITG"
     gene            complement(575..2926)
                     /locus_tag="AC16V7_11970"
     CDS             complement(575..2926)
                     /locus_tag="AC16V7_11970"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043885 - anaerobic carbon-monoxide
                     dehydrogenase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HIR6257881.1"
                     /translation="MTKEVMGLGHSRKRVEDARFLRGKGNYIDDFKLPGMLHLDIVRS
                     PYAHAKIKSINGEKATAIPGVLAVIDGKLLQQYNVHNMPTLMSDVQNVLPIDTVKYQF
                     QEVAAVLATDRYTAADGVAAVEVDYEPLPVMVDPKKALEPGAMKVREDKDSNHIWHWE
                     WGNREATNADISAAEVVVKEDIYIPRIHVSSIETCGCVAKWDSVEGHLTLWMTTQAPH
                     AIRTVVALITAHLGLSEEKIRVISPDIGGGFGGKVPAYPGYVIAVAASIVTGAPVKWI
                     EDRTENLAADSFGRDYHITAELAGKRDGTITSLRIKTIADHGYTDAAANPSKFPAGLF
                     HICTGSYDLKNAFVEVDGAYTNKPPGGIAYRCSFRVTEAVHTIERMADLYAHEIGMDP
                     ADFRMKNFIKPEQFPYKSALGWEYDSGDYHSALRLAMEIVGYEELRKEQEEKRARGEF
                     MGIGISSFTEIVGAGPSKHFDILGLKMFDSCEIRVHPTGKAIARFGTKSQGQGHETTY
                     AQIIAEELGIPADHIKIEEGDTDTAPYGLGTYASRSTPTAGAAAAIASRKIRDKARKI
                     AAHLLEAAEEDLIWEQGKFYVKGSPDQAKTIQEIAFAAYTNHPQGMEAGLEAVNYYDP
                     PNLTYPFGSYVAVVDIDRGTGEVKVRRFVAVDDCGVRINPMIVEGQIHGGLAMGFAPA
                     MMEEIQYDENGNIYGSTFMDYLLPTAVETPKWELGETVTPSPHHPIGAKGVGESATVG
                     APAAIVNAVVDALWHIGVRHVDIPIKPSRVWELLNEHGAAEYK"
     gene            complement(2919..>3145)
                     /locus_tag="AC16V7_11975"
     CDS             complement(2919..>3145)
                     /locus_tag="AC16V7_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR6257882.1"
                     /translation="EGFYEKHGLQCGFCTPGMLMTSSALLESNPNPTEDEIRWGISGN
                     LCRCTGYVNIVKAVQYAADKMQQEQGGSHD"
BASE COUNT          621 a          918 c          860 g          746 t
ORIGIN      
        1 cctgtgatct gcagcgcgga aaaatcgagc tgatcctgaa ggtgcatggc acctgggaag
       61 cgttccaatg agacttcgta tccagctacg gaaaccgggt agcccatcgc cctgccgagc
      121 ttgaccaggg attcagctgt cgctaagtgc cccacaacca ccaaccgggg ctgaacgagc
      181 tggggttcta tgaagatctc ataggttccc ccgctgatgc aggttagtgc gatctcgacc
      241 acgccttcct gtgcacccgt tccaagagat tccggcgggc acaggcgcag caatctgggc
      301 ttaccatcag caagcgcacg cagggcttct ttcttgaccg atggctccgc acagcttccg
      361 ccgacccagc ccttcagcgt gccatccgca gttatcagcg ctttggcgcc cggttttgca
      421 gaggttggct tttccgcacg cacgacgacc gcagttgcaa agggctcacc ctgctcggca
      481 agagaattta tgagtacgag gtgttctgtg atcataactg gagcctgtaa tcccccgggg
      541 tgctcaggca ctccggggga tgatttattg agagttactt atattctgct gccccatgct
      601 cattgaggag ctcccaaacc cgggaaggct tgatgggaat atccacgtgg cgaaccccaa
      661 tatgccagag cgcatctacc acagcgttta caatcgcggc gggggcgcct accgttgcag
      721 attctcccac gccttttgcc ccgatggggt ggtggggtga cggggtgact gtctcgccca
      781 gctcccattt gggggtctct acagcggttg gcagcaggta gtccatgaag gtgctgccat
      841 agatattccc gttttcatcg tattggatct cttccatcat tgcgggggca aaacccatgg
      901 ccagcccgcc gtggatctgc ccttccacga tcatcggatt gatgcgcaca ccgcagtcgt
      961 ccaccgctac aaatcgacgg actttgactt cgccagtacc cctatcaata tccaccacag
     1021 cgacgtagga accgaaggga tacgtcaggt tgggtgggtc atagtagttc accgcttcca
     1081 aaccggcttc catcccctgg ggatggttgg tgtatgcagc aaaagcgatc tcctggatgg
     1141 tcttcgcctg gtcgggagag cctttcacgt agaactttcc ctgttcccag ataaggtcct
     1201 cctcggcagc ttcaagcagg tgagcagcga tctttcgggc tttatcccgg atcttgcgag
     1261 aagcgatagc ggccgcggcg ccagcggtag gggtgctccg gctagcatac gtgccgagac
     1321 catatggagc agtatccgta tcgccttctt cgatcttgat gtgatcggct gggataccca
     1381 gctcctcggc gatgatctgc gcataagtag tttcgtgtcc ttgaccctgt gacttggtgc
     1441 caaagcgtgc gatggctttg ccagtcgggt ggacgcgtat ctcgcaggag tcaaacattt
     1501 ttaagccgag aatgtcaaaa tgcttggatg gtccggcacc gacgatctcg gtgaaggaag
     1561 agatcccgat gcccatgaac tcaccgcggg cgcgtttctc ttcttgctcc ttgcgtagct
     1621 cttcatagcc cacgatctcc atggccaggc gcagggcgct gtggtaatca ccgctgtcat
     1681 actcccatcc aagcgcagat ttatacggga actgttctgg cttgatgaag ttcttcatcc
     1741 tgaaatcagc ggggtccatg ccaatctcgt gggcatacag gtccgccata cgctcaatgg
     1801 tgtggacagc ctcggtcacc cggaaggaac agcggtaagc gatcccgcca ggcggtttat
     1861 ttgtataggc tccatccacc tcaacaaagg cgttcttcag gtcgtaggat ccggtgcaga
     1921 tatggaaaag accggcaggg aacttggacg ggttagctgc agcatccgtg tagccgtgat
     1981 cggcaatggt cttgatgcgt agcgaggtaa tcgttccgtc acgcttcccg gccagctctg
     2041 cagtgatgtg ataatcgcgc ccgaatgaat cggctgccag gttctcggtg cggtcctcga
     2101 tccatttaac cggcgcgccg gtcacaatcg aagccgccac agcaatcaca tagcctggat
     2161 acgccgggac cttaccaccg aaaccgccac caatgtcggg cgagatcact cgtatttttt
     2221 cctccgacag tcccaggtgg gccgttatga gcgcaaccac tgtccgtatg gcgtgcggcg
     2281 cttgagtggt catccacagg gtgagatgcc cctcgacaga atcccacttc gccacgcatc
     2341 cgcaggtctc tatggaggag acgtggatac gggggatgta gatatcctct ttgacgacca
     2401 cttccgcggc gctgatatcc gcattagttg cttcgcggtt accccattcc cagtgccaga
     2461 tgtggttgct gtccttgtct tcccgcactt tcatcgcgcc cggttctaat gcttttttcg
     2521 gatccaccat gacgggaagc ggttcgtaat ccacctccac tgcggccaca ccatcggctg
     2581 cagtgtagcg gtctgtcgcc agaacagccg cgacttcctg gaactggtat ttgaccgtgt
     2641 cgatgggcag cacgttctgg acgtcgctca tcagtgttgg catattgtgc acattgtatt
     2701 gttgcagaag tttgccatct attacggcga gtacgccagg tatcgccgtg gccttctcgc
     2761 cattgatact cttgattttc gcgtgggcat acgggctgcg gacgatgtcg aggtgcagca
     2821 tccctggcag cttaaagtca tcaatataat ttcctttgcc gcgcaggaag cgcgcgtctt
     2881 ccactcgctt ccggctatgg cccaagccca ttacctcttt agtcatggct gcctccttgt
     2941 tcctgctgca tcttgtctgc tgcgtattga acggctttca cgatgttcac ataacctgta
     3001 caccggcaca ggttgccaga aattccccag cggatctcat cctcggtcgg attggggttc
     3061 gattccaaca gcgccgatga ggtcatcagc attcctggtg tgcagaaccc gcactgcaag
     3121 ccatgcttct catagaaacc ctcct
//
LOCUS       DBNHOH010000690         3141 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1003776, whole genome shotgun sequence.
ACCESSION   DBNHOH010000690 DBNHOH010000000
VERSION     DBNHOH010000690.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1003776"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="AC16V7_11980"
     CDS             <1..1051
                     /locus_tag="AC16V7_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HIR6257883.1"
                     /translation="IFALLLYGGQGSIYVLYAVAMIGIGMLSHTGNNVAMDSYGPISD
                     NANGIGEMAWHDLEDESTMKARQIMADLDAVGNTTKAITKGIAIASAVIAAVSLFASY
                     ITDVGRVQEQLGVPMMESIRVSDTRVFIGLLLGGALPWLFSSLSIRAVSRAAGLIVLE
                     VRRQFRIPGIMEGKVKPDYARVVGISTAAAQKELIPLAVISVMLPLAVGLVLQVEALG
                     GFLAGIILSGQLLAVFMSNAGGAWDNAKKSIEDEDRDLEANTGKGSERHKAGVVGDTV
                     GDPLKDTAGPALNPMIKVTNLVSLIAAPIIVKYSSPNAIVIVVVVLLLASIVWAINQS
                     KKPAEEISQEVVPGD"
     gene            1252..>3141
                     /locus_tag="AC16V7_11985"
     CDS             1252..>3141
                     /locus_tag="AC16V7_11985"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6257884.1"
                     /translation="MAKIPLRAYNREIEKLIDQNQLEQAIAHARHILNYFPKHVNSYR
                     LLGKAYLESQRYGDAADVFQRVLSSIPDDFISHVGMSIIREDEANLDHAIWHMERAFE
                     VQPANGAIQGELRRLYGRRDGMEPPKVRLTRSALARMYIKGELFQQAISEVRAALAED
                     PQRPDLLSLLAQAYFQNGQRVEAANTCSSLLNKFPYSLEANRILAEILSSTERAEEAQ
                     VYRQRVQALEPYFGHTSPASPTPDLVSETAVVLEKLVWSPGQSVTGAQKQPEWAASLG
                     VELEATAPAEEGPPEWLADQPEAEPSATPEAHEEGDEAPSETPTPPSETPFAELPEDA
                     IPDWMKEAGWQSASGDVEEPVDILEEPAQDELTPAQIPDWLQSIAPGEKPEAEISDET
                     GESMPPIEETLSDSDALSWLDDAPPGPSDTVASWLDENQPAVPDISETESETEEKTEV
                     PAWLKDLQQPEPPSVSDETLTGTLTSPDEVSELEPQAQSLLPEIEAESDFAPSTTEAD
                     EGEKIPDWLQELGGAPSVEEEIEPTEELPDWLRQSDQEEWQTSEPSETATPETLSMPP
                     VEELPISSEDDELAWLDDLADKQGKKESIPLIPEDALAEAPDWVLEAALETPEILPTA
                     EDAPTS"
BASE COUNT          812 a          825 c          809 g          695 t
ORIGIN      
        1 catcttcgct ttattgctct atggaggcca gggttccata tacgttcttt atgccgtcgc
       61 catgatcggt atcggtatgc tcagccacac cggcaacaac gtcgcgatgg actcttacgg
      121 tccgatatcc gataacgcca acggcatcgg tgaaatggcc tggcatgacc tagaagatga
      181 atccaccatg aaagctcgcc agatcatggc tgatttggac gctgtaggga acactaccaa
      241 ggcaattacc aagggaatcg ccatcgcatc ggctgtcatc gcagcagtta gcctgtttgc
      301 ttcttatatt acagatgtgg gccgggttca ggaacagttg ggggtaccta tgatggaatc
      361 gatccgcgta tctgatacgc gcgtcttcat cggtttgctg ctaggtggcg cgttaccctg
      421 gctattcagc tcactttcta tccgcgctgt cagtcgtgca gctgggttga tcgtccttga
      481 ggttcgccgg caattccgta tccctggaat catggaaggc aaagtaaagc cggattacgc
      541 ccgggtagtt gggatctcga ctgcagccgc ccaaaaagaa ttgataccat tggcggtcat
      601 ctcagtgatg ctccctctgg cagtcggctt agtattgcag gttgaagccc tgggaggctt
      661 cctggcaggc atcatcctga gcggccagct tctggcagtc ttcatgtcga atgcaggtgg
      721 tgcctgggac aacgccaaga aatccattga agatgaagat cgcgatctag aagccaacac
      781 aggtaagggc tccgaacggc ataaagcagg tgtcgtcggc gataccgttg gcgacccact
      841 caaggatact gccggcccgg ctctcaatcc catgatcaag gtaaccaacc tggttagcct
      901 gattgcggct cctatcattg tcaaatacag ctcacccaat gcaatcgtta tcgttgtggt
      961 cgtgctgctg ctggcatcaa ttgtatgggc gattaaccaa agcaagaaac ccgctgaaga
     1021 aatctcgcag gaggtcgttc caggcgatta gccttgttat tttttggctt ttcagcatga
     1081 actacggagt gttgcggacg gtttaagccc gcaacgctcc atttttgtta gttttacttg
     1141 caactttgac cgatttgggg catacttaaa atcagctatg attggtagag aagcaatact
     1201 attaaaaagt agacctaatt tccgtcaccc ttaggcgagg gaacctgatt aatggccaaa
     1261 atacctttac gtgcttataa ccgcgagatc gagaagttga ttgatcaaaa tcaactcgaa
     1321 caagcaattg ctcacgcgcg ccatatcctg aattatttcc caaaacatgt caattcatac
     1381 cgactactgg ggaaagccta cctggaaagc cagcgctatg gcgatgcagc cgatgtcttt
     1441 cagcgcgtgc tctcctcaat ccccgatgac ttcatatccc atgtgggtat gagcataatc
     1501 cgcgaggatg aagcaaatct ggatcatgcc atctggcata tggaacgcgc cttcgaagtt
     1561 cagccagcca acggcgctat ccagggtgaa ttgcgccggt tgtatggtcg ccgagatgga
     1621 atggaaccac caaaggtccg gcttacacgc agcgcactgg cacgtatgta cattaagggt
     1681 gagctatttc aacaagccat cagtgaggtt cgtgctgcac tggcggaaga tccacaacgc
     1741 ccagatcttc tttctctact ggcgcaagct tatttccaga atggacagcg tgttgaggcc
     1801 gcaaacacct gtagcagtct cctcaacaaa tttccttaca gcctggaagc aaaccgcatc
     1861 ttagcagaaa tcctcagcag cactgagcgc gctgaagagg cgcaagtcta ccgccagcgc
     1921 gtccaggctc ttgagcccta ttttgggcat acctctcccg cttcacctac accagatctg
     1981 gtgtctgaga cagccgtggt gcttgaaaag ctggtatgga gccctggtca atcagttaca
     2041 ggcgcacaaa aacaaccaga atgggccgcc tccttgggtg tcgagctcga agccactgca
     2101 ccggcggaag aagggccacc agagtggctt gcagatcaac ctgaagcaga gccatcagcg
     2161 acacccgagg cacatgagga gggggatgaa gctccttcag agacgcctac cccaccatcc
     2221 gaaaccccat ttgctgaatt acccgaggat gcaatccccg actggatgaa ggaagcgggg
     2281 tggcaatctg cgtccggtga tgtagaagag cctgttgata ttctggagga gcctgcacaa
     2341 gatgagttaa cccctgctca aatcccagat tggctgcaat ccattgctcc aggggagaaa
     2401 cccgaagctg aaatcagtga cgaaacgggt gagtcaatgc caccgattga agaaaccttg
     2461 agtgacagcg acgctctctc ctggctcgat gatgcacccc ctggtccttc ggataccgtc
     2521 gcttcctggt tagatgaaaa ccaacccgcc gtacctgata tatctgagac cgaatcagag
     2581 acggaagaaa aaaccgaagt tccggcatgg ctgaaggatt tgcaacaacc cgaaccacct
     2641 tcggtatcag acgaaacgct cacaggcacc ctgacatcac ctgacgaagt tagcgaattg
     2701 gaaccccaag cacaaagcct tttacctgag attgaggctg agagtgattt cgctccatca
     2761 acaactgagg ccgatgaggg cgagaaaatt ccagattggt tgcaggaatt gggtggggca
     2821 ccgagtgttg aggaggaaat cgaaccaact gaagagctac ctgattggct tcgccaatcc
     2881 gatcaggaag aatggcaaac tagcgaacca tcggagacag caacgccaga aacgctttca
     2941 atgccacctg tagaggaatt gcccatttca tccgaagacg atgaacttgc ttggcttgat
     3001 gacctagcgg acaagcaagg taaaaaggaa tctataccac tgatcccaga agatgcgctc
     3061 gctgaagctc ctgattgggt gttggaagca gccctagaaa ccccagaaat attgcccact
     3121 gccgaggatg ccccaaccag c
//
LOCUS       DBNHOH010000691         4249 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_683199, whole genome shotgun sequence.
ACCESSION   DBNHOH010000691 DBNHOH010000000
VERSION     DBNHOH010000691.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_683199"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="AC16V7_11990"
     CDS             complement(<1..266)
                     /locus_tag="AC16V7_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR6257885.1"
                     /translation="MFEYTRSLRRDFHRHPELGFQEVRTAGIVAKELAELGLEVVSGV
                     GKTGVVALLEGKRPGPVVLVRFDMDALPIVEESEVDYASQNLGV"
     gene            complement(356..1303)
                     /locus_tag="AC16V7_11995"
     CDS             complement(356..1303)
                     /locus_tag="AC16V7_11995"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSP1 domain-containing protein"
                     /protein_id="HIR6257886.1"
                     /translation="MAPIIVGIRFQKIGKVYHFDASRFPDLQVGDQAVVETSRGAQLG
                     EVVQILEDPQPPPEGNWKSIQRRATPRDLVLRQIWQKKELEVTINCRAKAAEVKLDGV
                     KIVGADFSFDGSRLSILYSTEAETKIDTKRLRSAMQRMYPRSRVEMRLIGPRDVAKIL
                     GGMGACGMVNRCCSMFLTEFSPISIKMAKEQGISLTPSEITGMCGRLRCCLVYEYEQY
                     VEARKQLPKRNKRVVTPQGEGKVLDIYPLKESVLVELDRGFQQEFPQAELQPWDELEA
                     LRRKSKSPCDKHEGGQCDCGKEKKEPTQPRKMGRGHKNR"
     gene            complement(1390..2472)
                     /locus_tag="AC16V7_12000"
     CDS             complement(1390..2472)
                     /locus_tag="AC16V7_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HIR6257887.1"
                     /translation="MGKSVVTEAAVMAALGKVQEPELHRDLVSLNMIRDVQIDGERVS
                     FTVMLTTPACPMRSQIEQDARSAVATLPGVSEVDIDMSANVPSDGRPRGLLDLPIRNA
                     VAIASGKGGVGKTTVAVNAAVVLAQSGARVGLLDADIYGPNVPTMMGLQKLPAAQADK
                     LQPAEAFGVKVMSIGFLVKPDQPLIWRGPMLHSAIRQFLTDVNWGELDYLIIDLPPGT
                     GDAGLSLAQSLPLSGGVIVTLPQQVSLDDARRGLEMFRQLDVPIFGVVENMSYLELPD
                     GNRVDVFGSGGGERLAQESGVPFIGAIPMDPSVRAGGDNGTPVTISHPESAVASALTR
                     VAMDIAAKISVAAMQQGSFIPINLIG"
     gene            complement(2516..3124)
                     /locus_tag="AC16V7_12005"
     CDS             complement(2516..3124)
                     /locus_tag="AC16V7_12005"
                     /inference="COORDINATES: protein motif:HMM:NF024247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR6257888.1"
                     /translation="MDGNGILKGLGVTLRRFVDTYLEDLRWWGKRYYTQEGIAHRQSN
                     KTRGIFTVQYPEEKLPVPEEFRYIPFLLYEEGTNGDIVERCTSCGICAKVCPPQCIWI
                     VRTSDPNTGRPVPEPKEFYIDVDICMNCGFCSEYCPFDAIKMDHDYELAVYDRHQHNI
                     FDKDKLMKPVSYYASIRPTNYNREEEARAAAEAAKAARKAQA"
     gene            complement(3138..4241)
                     /locus_tag="AC16V7_12010"
     CDS             complement(3138..4241)
                     /locus_tag="AC16V7_12010"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HIR6257889.1"
                     /translation="MKTDQVVVNLGPQHPSTHGVFRIVVTLDGETVLSLKPVMGYLHR
                     NHEKIGERNTFLHNMPFTDRLDYLSSMSNNFGYALAVEKLMGIKPPERAEYLRVIMAE
                     LTRISNHVWAIGFLLNDLGAYFTPALYFIQERELILDIFEAVSGSRMMCNYFRFGGVA
                     RDLPEGVYEKIREIVYERLPRKVDEMDTYLTNSEIIRTRGEGVGVLTPEQAIAYATCG
                     PVLRASGVPYDVRRADPYSIYDRFEFDVAVRYHGDVYDRYLIRMDEIRQSLRILQQAL
                     RDLPEGSIQEGKPQYQVRVPAGESYGRVEGPKGELGYYVISNGKPNPWRYHVRAPSFI
                     NLTAMETMCIGNKIADVVAILGSIDIVLGETDR"
BASE COUNT         1023 a         1215 c         1026 g          985 t
ORIGIN      
        1 ataacaccca agttttgtga ggcgtaatcc acttcgctct cttcaacgat tggcagtgca
       61 tccatatcaa agcgcaccag aaccaccggg ccggggcgct taccctccag cagggcgact
      121 acaccggtct tgccaacccc agagacgact tccagcccta gttctgccag ctctttagca
      181 acgatccctg ctgtgcgcac ttcttgaaaa cccagctcgg ggtgacggtg aaaatcacgc
      241 cgaagggatc gggtatattc aaacaaattt tccgcttcta taagaaaatc actcatggac
      301 ataaaaaaaa ttagacccta aaagcttgct tgcttttagg gtcttccttt tcggattaac
      361 ggtttttgtg gccacgaccc atcttgcgag gttgggtggg ctcctttttc tccttgccgc
      421 aatcgcactg cccgccctcg tgtttatcac agggtgactt cgattttcta cgcagagctt
      481 ccaattcatc ccagggttga agctcagcct gcggaaattc ctgctgaaac cctcgatcca
      541 actcaaccaa gacagactcc ttgagcgggt agatatccaa caccttcccc tcaccttggg
      601 gtgtaaccac acgcttattg cgtttgggga gttgtttgcg ggcttccaca tactgctcat
      661 attcatacac cagacagcaa cgcaaccgcc cgcacatacc tgtaatctcc gatggggtga
      721 gggagatacc ctgctccttt gccatcttta tcgatatagg gctgaactcg gtcaggaaca
      781 tcgagcaaca acggtttacc ataccgcaag cccccatgcc acccaggatc ttggctacat
      841 ctcgaggacc gatgagacgc atttccacac gggagcgcgg gtacatgcgc tgcatggcgg
      901 aacgcagcct ctttgtatcg atcttagttt ccgcctcagt gctatataga atggataagc
      961 gtgatccatc gaacgagaaa tcagccccaa cgatcttgac gccatcaagc tttacctcag
     1021 ctgcctttgc acggcagttg attgtgactt ccagctcttt cttctgccaa atctggcgca
     1081 ataccagatc gcgcggagtt gcgcggcgct ggatagattt ccagttgcct tcgggtggag
     1141 gctgtggatc ttccaggatc tgtactacct cacccagttg ggcgccgcga ctggtctcca
     1201 ccaccgcctg gtcgccaacc tgcaagtctg gaaatctgct ggcatcgaag tggtagacct
     1261 taccaatttt ttgaaaacgt ataccgacaa taatgggagc cataataatt agccactata
     1321 gaacagttga cggctagtat aaaccggttg ccgccaactg acaactacaa tcctgcaaca
     1381 tgaaataatc tagccgatta aattgatcgg gataaagctt ccttgctgca tggcagccac
     1441 actgatttta gcagcaatat ccatcgccac acgggtgagg gcgcttgcaa ccgcagattc
     1501 agggtgggag atagtcactg gcgtgccatt gtcgcctcca gcacgcactg acggatccat
     1561 gggaatagcc ccaataaaag gcacgcccga ctcttgcgcc agccgctcac ctccaccgct
     1621 cccaaagaca tctacccggt tgccatcggg aagctccaaa tagctcatgt tctcgacaac
     1681 gccaaagatg ggaacatcta actggcggaa catctccaag ccacggcgag cgtcatccag
     1741 tgatacctgt tggggcaagg taacgatgac gcctccgctc aatggcaagg actgggccaa
     1801 gctcaatcca gcatcaccag tccctggagg caagtcgatg atcaggtaat ccagctcgcc
     1861 ccaattgaca tcggtgagga attggcggat agcggagtgc agcatgggtc cacgccagat
     1921 tagcggctgg tcgggcttga ccagaaaacc gattgacatc accttgacac caaacgcctc
     1981 agcgggctgt agcttgtcgg cttgcgcagc gggcagcttc tgcaagccca tcatcgtggg
     2041 cacgttcggg ccatagatat ctgcgtccag caatcccaca cgagcgccgc tctgcgccag
     2101 tactacggct gcgttaacag ctacggtagt cttacctaca ccgcctttgc ccgaggcaat
     2161 tgccacagca ttgcggatgg gcagatccag cagcccgcgc ggccgtccat cgctgggcac
     2221 attggcagac atatctatgt ctacctcgct cacgccaggc aaagtcgcaa ctgccgaacg
     2281 cgcatcctgc tctatctggg atctcatcgg acaggcaggt gtcgtcaaca taacggtaaa
     2341 actaaccctc tcaccatcaa tttgtacatc gcggatcata ttcaaagaga ccaagtcgcg
     2401 gtgcagctcc ggctcttgaa ccttgccgag cgctgccata actgctgcct cagtcacaac
     2461 actttttccc ataacaacca tcctataaac ttgtcgctct gactgcagcc atcagctatg
     2521 cttgtgcctt gcgagcggct ttcgctgcct cggcggctgc acgtgcctct tcctcgcggt
     2581 tatagttcgt tgggcggatg gaggcataat aagacaccgg cttcatcaat ttatctttat
     2641 cgaaaatatt atgctggtgg cgatcgtaca ccgctaattc gtaatcgtga tccattttga
     2701 tcgcatcaaa gggacaatac tcagaacaaa atccacagtt catgcaaata tccacgtcga
     2761 tgtagaactc tttgggctct ggaactggac gcccggtatt ggggtcagac gtgcgcacaa
     2821 tccagataca ctgcggaggg caaaccttag cgcaaatacc acaagaagtg cagcgttcaa
     2881 cgatatcgcc atttgtgccc tcttcataga gcaagaaagg aatatagcga aactcctctg
     2941 gtaccggcag cttctcttcg ggatactgaa ctgtgaaaat gccgcgcgtc ttgttgctct
     3001 ggcgatgggc tatgccctct tgggtgtagt aacgcttacc ccaccagcgc aaatcttcca
     3061 ggtatgtgtc cacgaagcgc ctcagggtga cgcctaatcc ttttaaaatc ccatttccgt
     3121 ccataatatc tccaatatca ccgatctgtt tcacccagga cgatatctat cgagcccagg
     3181 attgctacca catcggcaat cttgttgcct atacacatgg tctccattgc cgttaagttg
     3241 atgaaagaag gggcgcgcac atgatatcgc cacgggttag gctttccgtt ggaaataacg
     3301 taataaccca gctcaccttt cggaccctct acgcgcccgt aagattcacc agccggtacg
     3361 cgcacctgat actgtggttt gccttcctgg atcgaccctt cgggcaggtc gcgcagcgcc
     3421 tgttgcagga tgcgcaagct ttggcgaatc tcatccatgc gtatcaggta gcgatcgtaa
     3481 acatcgccat ggtagcggac agccacatca aattcgaagc ggtcatagat gctgtaagga
     3541 tcagcccggc gcacatcata aggaacacct gaagcacgca gcactgggcc acatgttgca
     3601 taagcaatgg cttgttcagg ggtaaggacg cccactcctt cgccacgcgt gcggatgatc
     3661 tcgctgtttg taagataggt atccatctca tctaccttgc gcggcaagcg ctcatacact
     3721 atctcacgga ttttctcata gacgccttca ggcaggtcac gcgccacgcc accgaagcgg
     3781 aagtaattgc acatcatgcg cgagccagag accgcctcga agatatctaa gattagctct
     3841 cgttcctgaa taaagtagag agcgggggtg aagtacgcgc ccaggtcatt tagtaaaaag
     3901 ccaatcgccc agacatggtt gctgatacgg gtcaactctg ccatgatgac gcgcaggtat
     3961 tctgcccgct cagggggttt aatccccatc aacttctcta cagccaaagc ataaccgaag
     4021 ttattgctca ttgaagagag gtagtctaag cggtcggtga atggcatgtt atgcagaaat
     4081 gtattgcgct caccgatctt ctcatgatta cgatgcagat atcccatcac tggcttgagg
     4141 ctgagcaccg tctcgccatc gagtgtaacc actatgcgga aaacgccgtg cgtggatggg
     4201 tgttggggtc ccagatttac gaccacttgg tcggtcttca tgcctgatt
//
LOCUS       DBNHOH010000692         6233 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_362190, whole genome shotgun sequence.
ACCESSION   DBNHOH010000692 DBNHOH010000000
VERSION     DBNHOH010000692.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6233)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6233)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6233
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_362190"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            347..1318
                     /locus_tag="AC16V7_12015"
     CDS             347..1318
                     /locus_tag="AC16V7_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6257890.1"
                     /translation="MDDYVLKVDDLTMHYVTRVGEVSAVDNVSFTLKKGEAMGLVGES
                     GCGKTSIATSLLRLLPDNAIIKGGHIFLNSTDIVKLSEDEMRKIRWRRISMVFQAAMN
                     ALNPVYKVGDQIIEAMENHGLVTTNEAANEHVAGLFRLVGLDPSLMEQYPHEYSGGMR
                     QRAVIAMALACNPDLIIADEPTTALDVIVQDNLLREMMELQQKLGMSMIYISHDIAVI
                     AEVSNRIGVMYAGRLVELASAAEVFHHPQHPYTAGLMSAFPSIIGPKRELSTLPGEPP
                     DLLHPPRGCRFHPRCQFATDICKQEAPEFKDLGGEHYVACYHPMEAN"
     gene            1319..2356
                     /locus_tag="AC16V7_12020"
     CDS             1319..2356
                     /locus_tag="AC16V7_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6257891.1"
                     /translation="MTEGRWNVKHVNDDLPNGKMPLVQVSGLRKLYPLTTGLFQTSRS
                     YIHAVDDINFTIPHGESLGLVGESGSGKTTTGKLLVRLTDPTEGQIHIYTSAGDTEIA
                     HLRGSELKKFRRHVQMIFQDPYESLNPRRTIFDTIAEPLVVQGLGDISERERRVAELL
                     VWVGLAPPETFMFRFPHELSGGQRQRVAIARALIVNPTFVVADEPTSMLDVSIRTGIM
                     QLMQKLADRLGVTYLYITHDLAVARYMSNRLAVMYLGKIVEMGDTEEVLAKPLHPYTK
                     ALLSAVPVPDPTYARTIPEIKGSIAQPINPLPRCRFYDRCPIAAKVCEQNDHPPLLDK
                     GSGHQVACYLV"
     gene            complement(2360..3796)
                     /locus_tag="AC16V7_12025"
     CDS             complement(2360..3796)
                     /locus_tag="AC16V7_12025"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6257892.1"
                     /translation="MASATQFQEDLKAYRLRQNRLRIWLSLQWRQARETWGLFSKNRL
                     AVFGVALIFIYALMSITHPILMSTVWSKGVYDPHVGYDLEIFPHPSPPGLGHPLGTDT
                     LGRDVLSRLLAATTPTFTLALTAALTTAIFSTLFAAFSAYAGGLIQSIQTHVSDIFLL
                     LPAPLVMIIIGGTIDINPFVFGLIYGLIAGLGGAAIVMRSQALTIIRKPYIEAALVSG
                     ASGVQIIFKHIIPNMLPLAAVQMLISVTGAVFADGFSTFLGISRTRLNWGSMIYDSFT
                     YQAINPAITWNVLIPSALAISLFATAFYLIARGIHEVAEPRLRDWEAAPTIKKLLAEQ
                     LEQAKSPAKPIPPPSLDDTAPVRVAPRSRIVLDDQAAFAWLEYLAVSQGASEALLLRP
                     EERRSKPPEWVTQSGVGVPPPLQSEAKIKVDDPPIFQPAPSSDKSLSIHEQARQAIEQ
                     GDAYLRAGELQQALNAYIKAEKLIRDGK"
     gene            complement(3796..4962)
                     /locus_tag="AC16V7_12030"
     CDS             complement(3796..4962)
                     /locus_tag="AC16V7_12030"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6257893.1"
                     /translation="MLRFIIKRFLSGLVVLFLFQTAIFFAVQLILPGDFASHFALMLS
                     RDEIQELRQILGLDLPIWERYLNWLQNMVRGDMGRSFTLRGSGPTVIEVLKAVTPPTI
                     LVFGIGTVLAFLLGLWLGKVTGWRRPGLITGSITFGGIALYTSFPPWLAFLLVYFLST
                     QLGLLPRSFTNKLWQGAPTSISQIMLQMVFALLVVLAILLLIQWLVRRITRRSMPTIV
                     LLLLTAAGWYASWYWMDILPYAVDIARAAALPLVGYVLLSFGEIMLLMRTTMLDVMHE
                     QYVHTARAKGLTSKDVRDRHVARNAILPVMSGLVVRLPYLLTGAVMLERALNWEGVGT
                     ELFTAVGLQNILLVMGLILIIGAISLVARLLLDILQALLDPRLRTAGGELGGTL"
     gene            5378..5932
                     /locus_tag="AC16V7_12035"
     CDS             5378..5932
                     /locus_tag="AC16V7_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257894.1"
                     /translation="MKRITFSLGVGLFLFPLIAAGIYVLTVRAAALVRYDKTYFTPYY
                     GELYSSPGSVASAIELALHQDQSPLLAELTGLRFKMRPLQANPNARLMVLWKITDSGY
                     FQYLFFDVRNYHRIIYNIKKVNGRWVMVPYDAYYYLDSGDWLLFFSPAVAIWWSVLLV
                     VTMGVTVYRIASRFREQVFHPPES"
     gene            6061..>6233
                     /locus_tag="AC16V7_12040"
     CDS             6061..>6233
                     /locus_tag="AC16V7_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6257895.1"
                     /translation="MTNQAEKPLLEVHNLKTYFFTDDGVVKAVDGVDFYVNPGEVLGL
                     VGESGCGKSVTSLS"
BASE COUNT         1427 a         1607 c         1743 g         1456 t
ORIGIN      
        1 ccacggagta ctctggttac ttgttggaca tgtccaagtg gtggttgatc ttccccgcca
       61 gcgtggcgat cacattcctg tgcgcagctt tttatctggt agggcgcgct tttgacgaga
      121 tcatcaatcc caggctgcgc aggagataat atgagtttat ccttttcccc atcccccctg
      181 gccccccttc ccttagggaa ggggggaaga aaaggcgaaa tgaggtgctt gcggagcaca
      241 ctccgcaagc acctcatttc gccgatttct ccccgcccct tggggggcgg ggtcgggggt
      301 ggggggattt gtatatatct tcaagaaaat tgaaggagaa aatcctttgg acgattacgt
      361 tctgaaagtt gatgacctga caatgcatta tgtcacccgc gtcggcgagg tctcggcggt
      421 ggacaatgtg tcctttacgt tgaaaaaggg cgaagcgatg gggctggtcg gagagtctgg
      481 atgtggcaag acatccattg ccacttcatt gctgcgcttg ctgccagata acgccatcat
      541 caagggcggg cacattttcc tgaatagcac ggatatcgtg aaactgagcg aagacgagat
      601 gcgcaagata cgttggcggc gcatttcgat ggtcttccag gcagctatga atgccctgaa
      661 cccggtttac aaagtgggcg atcagattat cgaggccatg gagaaccacg gcttggtgac
      721 caccaatgaa gctgccaacg agcacgtggc cggtttgttc cgcctggtgg gtctggatcc
      781 gagtttaatg gaacaatacc cgcatgaata cagcggtggt atgcgccagc gcgctgtcat
      841 tgctatggcg ctggcatgca atccagacct gatcattgcc gacgagccca ctaccgccct
      901 ggacgtgatc gtgcaggata acctgctgcg cgaaatgatg gaattgcagc aaaaactggg
      961 aatgagcatg atctatattt cccatgatat tgccgttatt gcggaggtaa gcaaccggat
     1021 tggagtgatg tacgcggggc ggctggtcga gctggccagc gcagccgagg ttttccatca
     1081 cccgcagcac ccttatacag cgggtttgat gtcagctttt cccagcatca tcggacccaa
     1141 gcgagagctc tctactttgc caggtgagcc tccagacctg ctccacccgc ctcgcggctg
     1201 tcgattccac ccgcgttgcc agtttgccac agatatttgc aagcaggaag cccctgagtt
     1261 taaggattta ggtggggaac actatgtggc atgctatcat ccaatggagg ccaactaaat
     1321 gactgaaggg cgctggaatg tgaaacacgt taatgatgac ctaccaaatg ggaaaatgcc
     1381 gctggtgcag gtaagcgggt tgcgaaagct gtatccgctg accactggtt tattccaaac
     1441 ctcgcgctcg tacattcacg ctgtggatga tatcaacttc actatcccgc acggtgaaag
     1501 tctgggcttg gtgggcgaat cgggctcggg taaaaccacc acgggtaagt tgttagttcg
     1561 cctgacagac cccaccgagg gtcagatcca catctatacg agtgctggtg ataccgaaat
     1621 tgctcacctg cgtggctcgg agcttaagaa gttccgccgg catgtgcaaa tgatcttcca
     1681 agatccatac gaatcgctca atccacggcg aaccatcttc gatacaattg ccgagccgct
     1741 tgtcgtccag ggcctgggtg atatttctga gcgggaaagg cgcgttgctg agctattggt
     1801 ctgggtcgga ctggcgccac cggaaacttt catgttccgc ttcccccacg agctatccgg
     1861 cggacagcgg cagcgtgtgg ccattgcccg ggcgttaatc gtcaacccca cttttgtggt
     1921 agcagacgag cccacctcca tgctggatgt gtccatccgc actggtatca tgcagctcat
     1981 gcagaagctg gctgaccgcc tgggggttac ctacctctac ataacccatg acttggcggt
     2041 ggcgcgctat atgtcgaacc gcctggcagt aatgtatctg ggtaagatcg tggagatggg
     2101 tgatacagaa gaagtgcttg caaagccgct tcatccttat acgaaggcgc tgctctccgc
     2161 tgtgccggtt cctgatccaa cctatgcgcg taccatccca gagattaagg gtagcattgc
     2221 ccagccgatc aatcccttac cgcgctgccg gttctacgac cgctgcccga tagctgccaa
     2281 agtgtgtgag caaaacgacc acccaccctt gcttgataag ggtagcggac accaggtggc
     2341 ctgctacttg gtgtaaatat tacttcccat ctcgaatgag tttttctgcc ttaatgtagg
     2401 cgttgagcgc ttgctgtagt tcccctgcgc gcaggtatgc atcgccttgt tcgattgcct
     2461 ggcgtgcctg ttcgtggatg gagaggcttt tatcggagct gggggctggc tggaaaatgg
     2521 gggggtcatc aacctttatt ttcgcctcag attgtagcgg gggtggaaca ccaacgccag
     2581 attgtgttac ccattctggg ggcttgctac ggcgttcctc aggcctgagc agcaatgctt
     2641 cgctggctcc ctgactcacc gccaggtatt ccagccaggc aaatgcggct tgatcgtcta
     2701 gaacaatacg actccgaggg gcaacacgca ctggtgcggt atcgtccagg gatggtggcg
     2761 ggataggctt ggctggcgat ttagcctgct ctagctgctc tgcaagcagt ttttttatgg
     2821 tgggtgcggc ttcccaatcc ctcaggcgtg gttcagccac ttcgtggatg ccgcgggcga
     2881 tcaaatagaa agccgtcgca aacaatgaga ttgccagtgc ggaaggtatc aacacgttcc
     2941 aggtgatggc gggattgatt gcctggtagg tgaaactgtc atagatcatg ctgccccagt
     3001 tgagcctggt gcgtgatatc cccaggaaag tgctgaagcc gtcggcgaag actgccccgg
     3061 tgacactgat cagcatctgt accgcagcca gcgggagcat attcgggatg atgtgcttga
     3121 agatgatctg taccccacta gccccgctga ctagggcagc ctcaatgtag ggctttcgga
     3181 tgatggtgag cgcctgagag cgcatcacga tcgccgcgcc ccccagcccg gcaattagcc
     3241 cgtatattaa accaaagaca aacgggttga tgtctattgt gccaccaatg atgatcatga
     3301 ccagcggggc aggtagcagc aagaagatat ccgaaacatg cgtttggata gactggatta
     3361 agccaccggc gtaggcagag aaggccgcga agagtgtgct gaagatagct gttgtcaggg
     3421 cggcggtgag tgccagggtg aaagtaggtg tggtggctgc aagtagccgg ctgagcacat
     3481 cgcgtcccaa tgtatcggtg cccagtgggt gtccgagacc tggcggtgag gggtggggga
     3541 agatttcgag atcgtagccc acatgcggat cgtatacgcc cttcgaccat accgtggaca
     3601 tcaggatggg atgggtgatg gacatcagtg cgtagatgaa aattaacgct accccgaata
     3661 ctgctaaccg gttttttgag aacaagcccc atgtctcgcg tgcctgtctc cactgcagcg
     3721 agagccatat tctcaagcga ttctgccgca ggcgataggc tttcaaatct tcctggaatt
     3781 gtgtggcgga ggccattaaa gcgtcccccc cagctcacca cctgcagtac gcaggcgtgg
     3841 gtcgagcagg gcttggagga tatccagcag cagccgcgcc accagtgaga tcgcgccgat
     3901 gatcaaaatc aagcccatga ccaataagat gttttgtaac ccgaccgccg tgaacagctc
     3961 tgtgccaact ccctcccagt tgagcgctct ttcgagcatg acagcccccg tgagcaggta
     4021 aggcagacgg acgaccagac cgctcatcac aggcaatatc gcgttgcggg ctacgtggcg
     4081 atcccgcaca tccttgctgg ttaaaccctt ggcgcgcgct gtgtgaacgt attgctcgtg
     4141 catcacgtcg agcatagttg tgcgcatcag cagcatgatc tcgccgaatg aaagtaacac
     4201 atacccgacc aggggcagag cggctgccct ggcgatatcc actgcatagg gaagtatatc
     4261 catccagtac cagctagcgt accatcccgc agctgtcagc aagagcaaaa cgattgtggg
     4321 catcgaccgg cgagtgatgc gacgaacaag ccattggatc agtagcagta tcgccagcac
     4381 gacaagtagg gcgaagacca tctgcagcat gatctggctg atgctcgttg gggcaccctg
     4441 ccatagtttg ttggtgaacg acctgggcag caagcctaat tgggtagaca ggaaatagac
     4501 cagcaggaac gccagccagg gtgggaacga ggtgtacaag gcgattccgc caaaagtgat
     4561 cgagccggtg attagtccgg gtcggcgcca gccagtgacc tttcccagcc acagtcccag
     4621 caagaaggct agcacagtgc caatgccgaa gaccaggata gtgggggggg taaccgcttt
     4681 taggacttct ataactgtcg gaccagagcc tctcaaggtg aaggaacgtc ccatatcgcc
     4741 tctcaccata ttctgcagcc aattgagata gcgctcccag atgggtagat ctagcccaag
     4801 tatctgacgc agctcttgta tttcatcgcg agatagcatc aaagcaaaat ggctggcaaa
     4861 atcgccaggg aggatcagct gcacggcaaa aaagatcgca gtctggaaca agaacaagac
     4921 aaccagaccg gacaagaatc ttttaattat gaaacgcagc atgatggacc ctttgcaact
     4981 ttggtgaccc cacccctttg gatattcacg atcttcagag cctataaacg ccttctgtct
     5041 ctaaataata ccatgtgatc actgctttag ttcatcatat ctcctttcct tggatctgcc
     5101 gttaccgacc agcacttctg gagtctcgac caaactaggg cagcgcacca tgaggattcc
     5161 tggtgaattt ggtgagatac atcagggcaa ttgcatatgc ccggaagggc atgcgcaatc
     5221 ccgcaaaaag agtgtttggg gtgttttggt gggggtatgc ccccaccaaa acaccccatt
     5281 ttctctcctc agtggtgggg aatcgcccaa atgcgattgc cctggagatg catagcatga
     5341 acatgtttat tcatggtaaa cttttaatga tgacaggatg aagagaataa ctttttctct
     5401 tggggttggg cttttcctgt ttccgctcat tgctgccggg atttatgtcc ttactgtgcg
     5461 cgcagcggca cttgtccgct acgacaagac gtactttacc ccatattacg gtgaactgta
     5521 ttcgtcccct ggctcggtgg ccagcgccat tgagctggct ctccatcaag atcaatcccc
     5581 tctcttggca gagttgaccg gcctgcgttt taagatgcgc ccgctgcagg cgaatccgaa
     5641 cgcccgcttg atggtcctct ggaagatcac cgattctggt tattttcaat atctcttttt
     5701 cgatgtaaga aactatcatc gcatcattta caatatcaag aaggtgaatg gtcgctgggt
     5761 catggtgccg tatgacgcct attactacct ggatagcggc gattggctgc tgtttttctc
     5821 gccggcagtc gccatatggt ggagtgtgct gctggtggtc actatggggg taacagttta
     5881 tcggatcgcg tcacgcttcc gtgaacaggt attccatccc cccgagagtt aaattatcat
     5941 gatagaattt atcgattcat tatcgccagg tgaatgcatc tggtatctgt tatctttatt
     6001 gcctgcgcat agttatgtcc ttaataggca gaaaagctga tcgctcaaag gagatagcac
     6061 ttgactaacc aagccgagaa gcctcttttg gaagtgcata atttgaaaac ctactttttc
     6121 accgacgatg gtgtagtgaa agccgtggat ggtgtggact tttatgtcaa cccgggtgag
     6181 gtgctgggtt tggtaggtga atcgggatgt ggaaagagcg tcacatcctt atc
//
LOCUS       DBNHOH010000693         2652 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_490750, whole genome shotgun sequence.
ACCESSION   DBNHOH010000693 DBNHOH010000000
VERSION     DBNHOH010000693.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_490750"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1600)
                     /gene="selB"
                     /locus_tag="AC16V7_12045"
     CDS             complement(<1..1600)
                     /gene="selB"
                     /locus_tag="AC16V7_12045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HIR6257896.1"
                     /translation="MRVIGTAGHVDHGKSTLVEALTGTHPDRLKEEREREMTIDLGFA
                     WLTLPNGEDVGIVDVPGHRDFIENMLAGVGGIDAALFVIAADEGVMPQTREHLAILDL
                     LQVQGGVIALTKVDLVEDEDWLSLVEDDVRQVVAGTVLGEAPIIRVSARKKIGLETLL
                     NAVGANLEEKPPRLDLGRPRLPVDRVFTISGFGTVVTGTLMDGCLRVGEEVEILPRGL
                     KGRVRGLQTHKKKEDVAIPGSRTAANITSVTVDQIRRGDVVAHPKDYSTTARMDVHFR
                     LLPDSSQPVRHNIQVKLFIGASEVVSRLRLLGRDELKPGETGWLQLELSEPVVAVRSD
                     RYILRRPSPGETLGGGSVVDPHPKGRHRRFSKDILARLEALAQGTPADIMMQALVAIG
                     AGPWKDVIARSNLNHAQVEAALDELRSTGALIVLDGASDGGVIQNESLVTSKGYWEQL
                     SKRALQELEQYHQVNPLRRGMPREELKSRLKVPPRLFATLISRLVASNQIHETGPIVS
                     VYGYEIRFDPEQQKMVQSLLARFAT"
     gene            complement(1607..>2652)
                     /locus_tag="AC16V7_12050"
     CDS             complement(1607..>2652)
                     /locus_tag="AC16V7_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257897.1"
                     /translation="KDLNTEITRLAGILARIDQFDESILQLRIEAKQSIDALEKQLKK
                     REDEIEKVRRVEMRALDDNIDEIRKDISAIGEIKRGMQARIDEEMRLGKSIDEVRLRI
                     DTIRRSEEEYTRSFRLLEDGRRQDAKRLSDLQGESTALRKHMDDLRGKMDLTSTSLRK
                     VETRLNEVSMLEVERREAQTKFLENQSLLQVERDRVWKDWQARFEVIEKQTSEVESNL
                     QSMDATHREVKRSQKTLEELNQLFERRINEMTEIQRLSEERFRQEWVTFKADDQKRWT
                     NYTLTQEEQRSESQRQYSKLSERVTQIEDHIQEIQDVIQQMNEQTEKRLQSLLANVHE
                     WVSAYERTLGRAR"
BASE COUNT          589 a          771 c          592 g          700 t
ORIGIN      
        1 aggtagcaaa tcgtgccaac agagattgca ccattttctg ctgttctgga tcgaaacgaa
       61 tctcgtaccc ataaactgag actataggcc cggtttcgtg tatttggttg cttgccacca
      121 gacgggaaat caaggtagca aacaatcgcg ggggaacttt tagacggctc tttaattcct
      181 cgcgcggcat gccgcgtcgc aacggattca cttgatggta ttgctctaac tcctggagag
      241 cgcgcttaga aagctgttcc caatagcctt tgcttgtcac caggctttca ttttgaatta
      301 cacctccgtc ggaggcgccg tctaaaacaa tgagcgcccc tgtggagcgc aactcatcaa
      361 gcgcggcctc cacctgggca tgattcaagt tggaccgagc gatcacatct ttccatggtc
      421 cagcaccaat tgccaccagg gcttgcatca ttatatccgc tggagtcccc tgggctagcg
      481 cttccaggcg agcgagtata tctttagaga aacgccgatg gcgacctttg ggatgaggat
      541 caaccacact cccccctcca agcgtttccc caggtgaagg acggcgcagg atataccggt
      601 cgctgcgtac cgctaccacc ggttcggaga gttcaagttg caaccatccc gtctcaccag
      661 gcttcagctc atcccttccc agcaagcgca aacggctaac tacttcagac gcgccgatga
      721 atagcttgac ttggatgtta tgtcgcacag gttggctcga atcgggaagc agacgaaaat
      781 gcacatccat gcgtgccgtc gtggagtaat cttttgggtg ggctaccaca tcaccccggc
      841 ggatttggtc cacggtcacg ctagtgatat ttgccgccgt ccgcgaacca gggatagcta
      901 catcctcctt ctttttatgt gtctgcaatc cacgcacacg accttttaac ccgcgtggca
      961 gtatctcaac ctcctctccc acacgtaaac agccatccat caaggtgccc gtgactacgg
     1021 taccaaaacc ggagatggtg aaaacgcggt cgaccggcaa gcgaggtcgc cccagatcca
     1081 gacgaggcgg tttttcctct aaattcgcac caacagcatt caagagcgtc tcgaggccaa
     1141 ttttttttcg agccgagacg cgaatgattg gggcttcgcc aagaaccgtt cccgccacca
     1201 cctggcgtac atcgtcctcg actagtgaca gccaatcttc gtcttcgacc aggtcaacct
     1261 tagttagcgc gataaccccg ccctgtacct gcaacagatc tagaatagcc aaatgctctc
     1321 gcgtttgcgg cataacccct tcatctgctg cgataacaaa tagcgccgca tcaatccctc
     1381 ctacaccagc aagcatgttc tcaatgaaat cgcgatgccc gggcacatcc acgatgccca
     1441 catcctcgcc attgggcaag gttaaccagg cgaaaccaag atcgatggtc atttcgcgtt
     1501 cgcgctcttc tttaagccgg tcggggtgtg tgccggtgag cgcttctacc aaggttgatt
     1561 taccgtggtc tacatgaccc gctgtgccaa ttaccctcat acttctctag cgcgctctcc
     1621 ctaatgtgcg ttcgtacgca gaaacccatt catgaacatt agccagcaag gactgcaatc
     1681 ttttctcggt ctgctcattc atttgttgga ttacatcctg aatttcctga atgtggtcct
     1741 caatttgcgt cacacgctcc gaaagctttg agtattgtcg ttgcgactcg ctacgttgct
     1801 cctcctgggt gagggtatag ttagtccacc gcttctgatc atctgctttg aatgtgaccc
     1861 attcctgccg aaaacgttct tcagataggc gctggatttc cgtcatctca ttgatacgcc
     1921 gctcgaataa ttgatttagc tcttccagtg ttttctggga tcgtttcacc tcgcgatgtg
     1981 tggcatccat tgactgcaaa ttggattcca cctccgaggt ttgcttttca atcacttcaa
     2041 agcgcgcctg ccaatctttc catacccgat cccgctccac ctgtaaaagc gactggttct
     2101 ccaggaactt tgtctgcgct tcgcgacgct ctacttccag cattgacact tcgttcaaac
     2161 gggtttcaac cttgcgcaga cttgtggagg tcagatccat tttgccacgt aaatcatcca
     2221 tatgcttacg cagcgcagtt gattcaccct gcaagtcaga taaccttttt gcgtcttgcc
     2281 ggcgcccgtc ttctaataag cgaaatgaac gagtgtattc ttcttcgctg cgccgaattg
     2341 tatcgattct taaccgcact tcatcgattg attttcctaa tcgcatttct tcatcaatcc
     2401 gagcttgcat accgcgcttg atctcaccga tcgcagaaat atcctttcgg atttcatcga
     2461 tgttgtcatc caaagcacgc atctccaccc ggcgcacctt ttcaatttca tcttcgcgtt
     2521 tcttaagttg cttttctagg gcatcgattg actgttttgc ctcgatacgc aattgcaaaa
     2581 ttgattcatc aaactggtcg atgcgagcca atatcccggc cagtcgagtg atctcggtat
     2641 ttaaatcctt ga
//
LOCUS       DBNHOH010000694         6587 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_298271, whole genome shotgun sequence.
ACCESSION   DBNHOH010000694 DBNHOH010000000
VERSION     DBNHOH010000694.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6587)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6587)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6587
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_298271"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..2166)
                     /locus_tag="AC16V7_12055"
     CDS             complement(136..2166)
                     /locus_tag="AC16V7_12055"
                     /inference="COORDINATES: protein motif:HMM:NF051749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HIR6257898.1"
                     /translation="MSDEQQETRLILAAEGWPAQSARPGQHTKAGFEVLAITAGNGNG
                     WEFSEEVLRASLSLWDGTSCFVDHAFWSGHSVLDLAGVFCNPRWSEEHRGILVELVTS
                     GPSGPLVDQLGWEMLREGKPKPRVGFSADLWFTCKGRVVERITRVESLDLVINPARGG
                     AFIRALNATRGTNSQPPSGGIQKGAKIAMDETTPTPDGRQAATPAGESLAQNELEAAR
                     SIPASQKEWQAAQQLRLQMCQYLLDSGLAAAKLPATVQEHLRKQFAGKLFEPDELQQS
                     IDSARELVSQLAGSSAVIGPGRVHGVFSSEDQLQAAVDDLMGAPRDKGMEGLKVARLS
                     GIRELYLMLTGDEELHGGYYPSRVRLATTADFAGLVKNALNKIVVNQWGELGRAGYDW
                     WQRISSVEQFSSLQSISGTLIGTVGTLPTVAEGAEYTELTVGDSPETANFTKYGGYIP
                     LTLELIDRDETRKLRAYPRELANAGLRKVSALVAALFSDNSGTGPTMADGGALFNATA
                     VTTAGGHANLRTTALSTAEWEAVSQAVYNQPMLIKNAAGVYGTGPKQAINPRYLLVPR
                     ALELTAKKILYPFWENAANIHSENQQRGEMGDVVVVPEWTDANDWAAACDPRIAPAIF
                     VGTRFGLMPEVFLAGSELSPAVFMNDEHRLKVRHFIAVWVNDFRPLHKSNVA"
     gene            complement(2179..3528)
                     /locus_tag="AC16V7_12060"
     CDS             complement(2179..3528)
                     /locus_tag="AC16V7_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257899.1"
                     /translation="MLSRFVGFFFGEEIQRQASLAVRVLDDKTDRLAASWSTYVSTYP
                     RDRHGYDRDEILRDALDAWRQNPLARRVVELMSQYVVGGGLGVECRHERTHRFLQNWW
                     NHRLNRMPLRAFDLCDELTRTGNLFIVVSTDAAGMSYIRTLPTQDILDIETALNDIEQ
                     ELVVWERPRGDGTEQATPNGFMGHAWPVYDERTDRQDADGSFPPAVLHYAVNRPVGAK
                     WGESDLAPLLRWLSRYSAWLEDRARLNRYRQSFLYVVKAAFTSQAERLARQAELNANP
                     PNPGAILVADESETWSVLNPELASFEAAEDGLAIKKMIAVGSGNPLHFLAEPESATRT
                     TAEAAGGPTFRHYEQRQKFFLWMIEDLARVVVQRRALVDHHITPRAYISAKGTDISAR
                     DNTSLAAAANTIVSAFALLRKRGLIDDAELLRMAYRFAGEVVDVEEMLRRGAAAGSI"
     gene            complement(3528..5129)
                     /locus_tag="AC16V7_12065"
     CDS             complement(3528..5129)
                     /locus_tag="AC16V7_12065"
                     /inference="COORDINATES: protein motif:HMM:NF036421.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HIR6257900.1"
                     /translation="MNELLTYLPTLKSLVRDVRLFARHAAKLSLRSYQQSVALAIVES
                     VIEGRGDTIVVMFPRQSGKNELQAQVESYLLMLFFMANAEIVKISPTWKPQSHNAMRR
                     LERVLSKNFFTKFNWKKESGYIYRVGTARIFFLSGSQEANIVGATASTLLEVDEAQDV
                     LVAKFDKDIAPMAASTNATRVFWGTAWNSRTLLARELRAAREAERRDALAGRPVRQRV
                     FVITADEVAAEVPAYGKFVAEQIAKLGRDHPMVRTQYYSEEIDAEGGMFPARRLALMQ
                     GSHPRQTAPSAQPGRIYALLIDVAGEDEAMRTDEGADAHVGLRAGSLANPRRDSTALT
                     VVEVDLSSLKDPVLRAPTYRVVDRRSWIGVRHSTLYAQIRALVEHWVARHIVIDASGV
                     GAGLASFLDRAFPNRVIPFVFNAATKSNLGWDFLTIVETGRFKDYATEESQRSAFNLQ
                     LSCCQMETLPGPDRRMKWGVPEGTSGGQDLDSSPGQAGKLIHDDWVLSAALCAVLDSL
                     EWRISTPTAVIQAVDPLKQMDREGF"
     gene            complement(5126..5503)
                     /locus_tag="AC16V7_12070"
     CDS             complement(5126..5503)
                     /locus_tag="AC16V7_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257901.1"
                     /translation="MEMNLNADEAKPEERPRRKRGAQPGNLNALKHGLYSRRLQEPGA
                     EADLEEEIITLRNLVQRTSQLATDPDMNLTTLLKVLDTIGSAMNRLANLLQAQKQLHK
                     SSTDANAVLLQAVIEVARELGIE"
     gene            complement(5523..5783)
                     /locus_tag="AC16V7_12075"
     CDS             complement(5523..5783)
                     /locus_tag="AC16V7_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257902.1"
                     /translation="MGLDRFVLNPTTLLVLIFGLVEYIKGFGVRGNSLRAVSMALGVA
                     LAVAYRLREAAPDWAGWIEMAFFGLAAGLAASGVYDFLKNRL"
     gene            complement(6062..>6587)
                     /locus_tag="AC16V7_12080"
     CDS             complement(6062..>6587)
                     /locus_tag="AC16V7_12080"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR6257903.1"
                     /translation="LGSHPQAYQRAQDEVDAFNFSGQVTIEQLSGLRYLDSVIREALR
                     LYPPIHLGSRLAATDLDYQGYRIPAGERVIYSIYLTQRHRGHWQDPHRFLPERHASGG
                     QKTPYTWLAFGGGPRNCIGAAFGQVETKLVLATVLRRFDLQLVERHVRPHMGATLEPH
                     PGVRMRLKLRTPSQ"
BASE COUNT         1366 a         2009 c         1887 g         1325 t
ORIGIN      
        1 ctctgctggc acagagtcag gttacgccac attgctctta tgcaatgggc ggcccgtctc
       61 tgctgacgca gagtcaggtt acgccacatt gctcttgtgc aatgggcggc ccgtctctgc
      121 tggcacagag tcaggttacg ccacattgct tttatgcaat gggcgaaagt cattgaccca
      181 cacggcgata aaatggcgga ctttcagccg gtgctcatcg ttcatgaaca cagcaggcga
      241 tagctcgctg cccgctaaga acacttcggg catcaggcca aagcgcgtgc ccacgaagat
      301 cgccggggcg atgcgcggat cgcaggcagc cgcccagtca ttggcgtcgg tccactcagg
      361 caccacgacc acatcaccca tttcgccgcg ctgctggttc tcgctgtgga tattggcggc
      421 gttctcccaa aacgggtaga ggatcttctt ggcggtcagc tccagggcac gcggcaccaa
      481 caagtagcgc gggttgatag cctgcttggg tcccgtacca tacacacccg cggcgttctt
      541 gatcagcatg ggctggttgt agacagcctg ggaaacagcc tcccactccg cggtgctcaa
      601 ggcggtcgtg cgcaggttgg catgacctcc ggcggtagtc acagcagtgg cgttgaacaa
      661 tgccccgcca tctgccatcg tggggccggt accgctgtta tcgctaaaca atgcagccac
      721 cagtgctgag accttgcgca gcccagcatt cgccagctcg cgcgggtatg cgcgcagttt
      781 gcgggtctcg tcacggtcga tgagctccaa cgtcaacggg atataaccgc cgtacttggt
      841 gaagttggcg gtttcggggc tgtcacccac ggttagctcg gtatactcgg ctccctccgc
      901 taccgttggc agggtgccca ccgtcccaat cagggtgccg ctgatggatt gcaacgagct
      961 gaactgctcc acgctagaaa tgcgctgcca ccaatcgtag ccggcgcgcc ctagctcacc
     1021 ccactggttg accacgatct tattgagggc gtttttaacc agtccggcga aatcagcggt
     1081 ggtagccagc ctcaccctag acgggtaata gccgccatgc agctcttcat cgccggtgag
     1141 catgagatac agctcgcgga tgccggacag gcgagctacc ttcagccctt ccatgccttt
     1201 atccctgggc gcgcccatta ggtcgtcaac tgcggcttgt agttgatctt cgctggaaaa
     1261 cacgccatgc acccggccag gcccaatcac agcggagctg ccggccaact gcgagaccag
     1321 ctcgcgagcc gaatcgatag attgctgcag ttcatccggc tcaaacagct tcccggcgaa
     1381 ctgcttacgg agatgctcct gcacagtagc aggcagcttc gcagccgcca ggccgctatc
     1441 cagcagatac tggcacatct gcaggcgtaa ctgctgtgcg gcttgccact ctttctggga
     1501 agctgggatt gagcgagcag cctccagttc attctgcgcc aaagattcac ccgccggggt
     1561 cgcagcctgc cttccgtcgg gggttggtgt ggtttcatcc atcgcgatct ttgctccttt
     1621 ctgtatgccc ccgctggggg gttggctatt tgtgccacgc gtggcattaa gtgcccggat
     1681 aaacgcgccg ccgcgcgccg ggttgataac cagatccaga gattcaaccc tggtaatccg
     1741 ttcgacaaca cgccccttgc aggtaaacca caggtcagcc gaaaacccta ccctgggctt
     1801 gggcttcccc tctcgcagca tctcccagcc aagctgatcc accaatgggc ccgaaggtcc
     1861 tgaggtgacc agttccacca gaatgccgcg gtgctcctcg ctccagcgag ggttgcaaaa
     1921 caccccggcc agatcgagaa cagaatggcc cgaccaaaag gcgtgatcta caaaacatga
     1981 tgtcccgtcc cagagagaca gggaggctct caagacctcc tcggaaaatt cccaaccgtt
     2041 gccatttcca gcggtgatgg ccaagacttc aaagccagcc ttagtatgtt gtccaggacg
     2101 agcggattgc gctggccagc cttcagcggc caggatcagg cgggtctctt gctgttcgtc
     2161 tgacatactt tctccttatt agatcgagcc tgccgcagcg ccccggcgaa gcatctcttc
     2221 cacatcgacc acctcgccgg caaagcgata cgccatcctc agcagctctg catcgtcgat
     2281 caatccccgc tttctcagca gggcaaaggc actcacgatg gtattggcag ccgcagccag
     2341 ggaggtattg tcgcgagcgc tgatatccgt ccctttggcg gagatgtatg ctctgggggt
     2401 tatgtgatga tccacaagcg ccctgcgctg cacaaccacc ctggctaagt cctcgatcat
     2461 ccacaaaaag aacttctgcc gttgctcata atggcggaag gtggggcctc ccgcggcttc
     2521 tgcggtagta cgggtggcgg attctggctc agccaggaag tgaaggggat tcccggatcc
     2581 gacagcgatc attttcttga tggccaggcc gtcctcagca gcttcgaagc tggccaactc
     2641 cgggttcagc acgctccagg tctcgctctc gtcagccacc aggatggcgc ccgggttagg
     2701 cgggttggca ttgagctcgg cctggcgcgc aagcctttcc gcctggctgg tgaaagcggc
     2761 cttgaccacg tacaagaagc tttgcctgta gcggttcagc ctggcgcgat cctccaacca
     2821 ggcggaataa cgcgatagcc agcgcagcaa gggagccaga tcgctctctc cccacttggc
     2881 gcccaccggc cggttgacag cataatgcag cacggctggc gggaagctgc cgtcggcatc
     2941 ctggcggtcg gtgcgctcgt catagacagg ccaggcatgc cccataaagc cattgggggt
     3001 ggcttgttcg gtgccatcgc cgcgcgggcg ctcccacacc accagctcct gctcgatatc
     3061 gttgagggcg gtctcgatgt caagaatatc ttgggtgggc aaggtgcgga tatatgacat
     3121 gcccgcggca tcggtggaaa ccacgatgaa cagattaccc gtgcgggtca gctcgtcaca
     3181 caggtcgaac gctctcaaag gcatgcggtt caggcgatgg ttccaccagt tctgcaaaaa
     3241 gcggtgagtg cgctcgtgcc tgcactccac acccagccca cctccaacga catattgaga
     3301 cattaattcg acgacacgac gggcaagcgg attttgtcgc caggcatcca gggcgtctcg
     3361 caggatctca tcgcggtcat agccatgccg gtcgcggggg taggtcgaca cataggtcga
     3421 ccaagacgca gccaggcgat ccgtcttgtc atcgaggacg cggacggcca aactggcctg
     3481 gcgttggatt tcctcgccga aaaagaagcc aacaaagcgc gaaagcatca aaaaccctcc
     3541 ctgtccattt gcttgagcgg atccaccgcc tggatgaccg cggttggggt agaaatacgc
     3601 cactccaggc tatccaggac agcacacagc gctgcagaca ggacccaatc atcgtgtatg
     3661 agcttgccag cctgccctgg cgaactatcc aagtcttgcc ctcccgacgt gccttcgggc
     3721 accccccact tcatgcggcg atctgggccg ggcagcgtct ccatctggca gcaggaaagt
     3781 tgcaggttga aggcagaacg ttggctttct tcagtagcat agtctttgaa acgaccggtt
     3841 tccacgatgg tcaagaaatc ccagcccaaa ttgctcttcg tggccgcatt gaacacaaag
     3901 gggatgaccc ggttgggaaa agccctgtca agaaacgatg ccagcccggc gcccaccccg
     3961 ctagcgtcaa tgacaatgtg acgggcaacc caatgctcta ccagggctct aatctgggca
     4021 tacaaggtgg agtgcctgac cccgatccag ctccttcgat cgacaacacg ataggtaggg
     4081 gctctcaaaa caggatcttt caagctagag aggtctacct cgaccacggt aagggcggta
     4141 gaatcacggc gcggattggc aagactgcct gcgcgcaggc caacgtgcgc atcagcccct
     4201 tcgtccgtcc gcatagcctc atcctcaccg gcgacgtcga tcagcagggc atatatccgc
     4261 ccaggttggg cggacggagc ggtctggcgt gggtggctgc cctgcatgag ggccagtcgg
     4321 cgggcgggga acatgccccc ttcggcgtcg atctcttcag aatagtattg cgtgcggacc
     4381 atgggatgat cccgaccgag tttggcaatc tgctcagcaa cgaattttcc gtaggccggc
     4441 acttcggcag cgacctcatc ggcggtgatg acaaagactc gctgccggac tggacgccct
     4501 gccagtgcgt ccctgcgctc ggcttcacgg gcggcgcgca gctcacgggc gagcagcgtg
     4561 cggctgttcc aggcggtgcc ccagaaaacg cgcgtggcgt tggtggaagc ggccataggc
     4621 gcgatgtctt tatcaaactt agcgaccaac acatcctggg cctcatcgac ttccagcagg
     4681 gttgaagcgg tggcaccgac gatgttggct tcctgggatc cagacaggaa gaaaatgcgc
     4741 gctgttccca cgcgatagat atatccagac tctttcttcc agttaaattt cgtgaaaaag
     4801 tttttagaca ggacacgctc caggcggcgc atggcgttgt gggattgagg cttccaggtg
     4861 ggagatatct tcacaatttc agcattggcc atgaagaaaa gcatcaaaag ataggactcg
     4921 acttgcgcct gcagctcgtt ttttcctgac tgccgaggaa acatgaccac aatggtgtct
     4981 cctcgccctt cgatcaccga ttccacaata gccagagcga cggattgctg ataggagcgc
     5041 aggcttagct tggctgcgtg cctagcgaag agccgcacat cacgcaccaa gctttttagt
     5101 gtaggcaagt aggttaacag ctcgttcact cgattcccaa ctccctggca acctcgatca
     5161 ccgcctgcag cagcaccgcg ttcgcatctg tggatgactt atgcagctgt ttctgtgcct
     5221 gcagcaaatt agccaggcgg ttcatcgctg agccaatggt gtccagcacc ttcaacaggg
     5281 tggtcagatt catgtcgggg tctgtagcca attgggaggt gcgctgtacc aggttccgca
     5341 gcgtgatgat ctcctcctcc aggtcagctt ccgcgcccgg ctcctgcagg cgtcgcgaat
     5401 ataagccatg ctttagggca ttcaggttgc ccggttgggc accgcgcttg cgccgtggcc
     5461 gttcttctgg cttggcttca tcagcgttca aattcatttc catctgcaca tccggccatg
     5521 aatcagagtc gattcttgag aaagtcatat acacccgaag cagccaggcc agccgccaga
     5581 ccaaagaagg ccatctcaat ccagccggcc caatccggtg cggcctctcg cagccggtaa
     5641 gccaccgcca gcgccacgcc cagtgccatg ctcaccgcgc gcaggctgtt gcctcgcacg
     5701 ccgaagccct tgatgtattc cactaaccca aagatcaaga ccagcaatgt ggttgggttc
     5761 aagacaaaac gatccaggcc catacatgct cctttctgcc atttagcccc ccgctctccg
     5821 gggtttccag aggcacagag accccggagg cgttaggagg gatatccagt gtgcacgcgg
     5881 agaacggcgc aaggttttct ggggaagtta aggcccgctt cgcgtccacg caggattgca
     5941 tgcgtatcgt agcaaaaaaa agtatttaga acaagtgttc tataacgaaa tcccccgaaa
     6001 tatcccccat ttttgcagac attttgagag ggttaaccag gtcgaaggcg gtgagagcag
     6061 cttactggct gggtgtgcgc agctttagcc gcatgcgcac gcccgggtgc ggctccaacg
     6121 tagcccccat gtgcggtcgc acatgccgct caaccagttg caggtcgaag cgacgcaata
     6181 cagtcgccag caccagcttg gtttccacct gaccaaaagc cgcaccgatg caattgcgcg
     6241 gtcccccacc aaacgccagc catgtgtaag gtgttttctg cccgccagag gcgtggcgct
     6301 caggcaggaa gcgatggggg tcttgccaat ggccgcggtg tcgctgggtg aggtagatag
     6361 agtagatcac ccgctcgcca gccgggatgc ggtatccctg gtagtccagg tcagttgccg
     6421 ccagacgcga gcccaggtgg atgggcgggt agaggcgcag ggcttctcgt atcacgctat
     6481 ccaggtagcg cagcccagac agttgctcaa tagtgacctg acctgaaaag ttaaacgcat
     6541 ctacctcgtc ttgggcgcgc tggtaagctt ggggatggct gccaagc
//
LOCUS       DBNHOH010000695         2650 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_554076, whole genome shotgun sequence.
ACCESSION   DBNHOH010000695 DBNHOH010000000
VERSION     DBNHOH010000695.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_554076"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1751
                     /locus_tag="AC16V7_12085"
     CDS             <1..1751
                     /locus_tag="AC16V7_12085"
                     /inference="COORDINATES: protein motif:HMM:NF037660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6257904.1"
                     /translation="FLSQRLRASEPDRIPELHRRASQWYENEGWVDEAIQHALAAGDM
                     DRATRLVDGIAVDLLVRAESNKLLKLVEQLPFDLWQGYPMLRILYAWALLFMGQLERV
                     DPILTSVEVDQAKAPGVPNPGYVTTVRAYLANRQGDIYRSIQLTEQALEEMSKATPDR
                     LTLKFQGSAVIWLGVNHRLLGNLDKARQLFMEAAGINQKAGNYYASLASYEQLAELAV
                     IRGQLHQALDLYRNGLNITQNWKDTRGKPRGTLMTTTGLQLGLGVVLYQLNDLAGAAT
                     QIQRSADLFELGEHYERKLSYTMLAYLKQAQGEFETSAELLRKACAIEDTMVVRRSYT
                     SDRSSPTKLAILLSRAGPEMAHLLTDASRRVMKLGVRSTDKVDFSSPADYPRELIYSD
                     LACLLIAQDRADEALPLLSSLLEAAITMGRHGDEIRYLVLMTLAHHALGDMQTALDFL
                     SRALTLAEPEGYVRLFVDEGPPMAELLNHAISHNISSDYASKLLAAFPNELLSVIPIA
                     KELTVTTQMLVEPLSEREIEVLHLMAEGCKYKEIAERLVVSINTVRYHTRNIYGKLNA
                     TNRTQAIGRAKELNLL"
     gene            1896..2174
                     /locus_tag="AC16V7_12090"
     CDS             1896..2174
                     /locus_tag="AC16V7_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257905.1"
                     /translation="MADEQKKYTSKNYKIKIQGHLDTHWSEWLYGMTITQKGDGTTTL
                     YGPLPDQTVLHSVLDRIRDMNLPLISVNQVELERQTSKDEAKGVSQDE"
     gene            2224..>2650
                     /locus_tag="AC16V7_12095"
     CDS             2224..>2650
                     /locus_tag="AC16V7_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257906.1"
                     /translation="MNTKHLLINIVLLAALLLAGCGAKETAPLAVPDGAQAGDLVGLE
                     SCTYEAQDAKYVADCGTLVVPENRADPNSRLLALPVIRVRATGSNPAEPIFWLEGGPG
                     RSNMGFPLGSWFIENHDFVLVGYRGMDGSVVLNCPEVVEL"
BASE COUNT          658 a          670 c          707 g          615 t
ORIGIN      
        1 atttcctgag tcagagactg cgtgcgtccg aaccagacag gatccccgag ctgcaccgcc
       61 gggccagcca gtggtatgaa aacgaggggt gggtggacga ggccatccaa cacgccctgg
      121 ccgccgggga tatggatcgc gcgacccgcc tggtcgatgg aatcgcagtc gatttattgg
      181 tgcgagcaga gtcgaataag ctattgaaat tagtcgagca gctacccttc gacttgtggc
      241 aaggttaccc gatgctacgc atcttgtatg cctgggcact gctttttatg ggacaactgg
      301 aaagggttga tccgatctta acctccgttg aggtggatca ggccaaagcg cccggggttc
      361 caaaccctgg ctatgtgacg accgtgcgcg cctatttagc caatcggcag ggggatattt
      421 atcgatcgat ccaacttacg gaacaggcgc ttgaagagat gtcaaaggct acgcccgatc
      481 gattgacgct caaattccaa ggatctgctg tcatctggct gggggtaaat catcgcctgc
      541 tgggtaatct cgacaaggca agacaacttt ttatggaagc cgctgggatc aaccagaaag
      601 cgggtaacta ttacgcatcc ttagcatctt atgagcaatt ggccgaactg gcggtgatcc
      661 gtgggcagct gcatcaggcc ttggatttat atcggaacgg attgaatatc acccaaaatt
      721 ggaaggatac gagaggcaaa cctaggggaa ctttgatgac aaccaccggg cttcagctgg
      781 gactcggagt ggtgctgtat caattgaacg acctggctgg cgctgcaact caaatccagc
      841 ggagcgctga cctcttcgaa ttgggcgaac actatgagag aaagctttct tacacgatgc
      901 tggcttatct gaaacaggct caaggagaat tcgagacttc tgctgaactg cttcgcaaag
      961 cctgtgctat cgaagatacc atggttgttc gacgatcata cacatcagac cgttcgagcc
     1021 cgacaaagct ggcaattctg ctcagccgcg ccggacctga aatggctcat ctgcttacgg
     1081 atgctagccg acgggtcatg aaactgggag tgcgttcaac cgataaggtt gacttttcca
     1141 gccctgccga ctaccctcgc gagcttattt actcagatct ggcctgtttg ctcatcgccc
     1201 aggatcgggc tgatgaagcg ctgcctttgc tatcaagcct gctggaggct gccattacga
     1261 tgggaaggca tggcgatgag atccgttatc tggtgttgat gactttggcg catcatgccc
     1321 tgggggatat gcaaactgca ctggacttcc taagtcgggc actgaccctg gctgagcctg
     1381 agggttatgt tcgacttttt gttgatgagg gaccgccgat ggcggaattg ctcaatcatg
     1441 ctatctcaca caatatctcg tccgactatg cgagcaaact tttagctgct tttcccaatg
     1501 agttattgag tgtaatccca atcgccaagg aactgaccgt cactacacaa atgctggttg
     1561 aaccactcag cgagcgagaa atcgaagttt tacatctgat ggcggagggg tgcaagtaca
     1621 aagagatcgc cgaaagactg gtcgtcagta tcaatacggt tcgctaccac acccgcaaca
     1681 tttatggcaa gctcaacgcc accaaccgca cccaggcgat tggcagagct aaagaattaa
     1741 acttgctgta gttcccccta catcttttcc aaaagaaaac tacatcattt ttcctcgcta
     1801 gccgcattaa catgcggtga ttttttttcc tgcttttgct cttctgggtc tgtagaaaat
     1861 agcacacggg aatacctccc agaatggaga aatttatggc tgatgaacaa aagaaatata
     1921 catctaagaa ctacaagatc aaaatccagg ggcacctgga tacccattgg tctgagtggc
     1981 tctacggcat gaccatcacc caaaaaggtg atggcaccac cactctgtac ggcccgctcc
     2041 ccgaccagac ggttttacac agcgtgctgg acaggatccg ggacatgaac ctgccgttga
     2101 tatcggtcaa tcaagtcgaa ctagaaagac aaacaagtaa agatgaagcc aaaggagtga
     2161 gccaagatga gtaacccgtt tttcgaaatc cacctatttg aacaaaaaaa ggagaaccgc
     2221 atcatgaata caaaacatct tttgatcaat atcgtacttt tggccgcgct gcttctcgct
     2281 ggctgtggtg ccaaggagac cgcgcccctg gcagttcccg acggcgcgca ggctggcgat
     2341 ctggtaggct tggaatcttg cacgtatgaa gcccaggatg ccaagtacgt tgccgactgc
     2401 ggcacgctgg tcgtgccgga gaaccgagct gatccgaact cgcggctgct tgcattgcct
     2461 gtcattcgcg tccgcgccac agggagcaat cccgccgagc caatcttctg gttagagggc
     2521 gggccaggtc gtagcaacat gggattccct ctcgggtcat ggtttatcga aaaccacgat
     2581 tttgtgttgg ttggctaccg gggtatggat ggttcggtgg tgttaaattg tccagaggtc
     2641 gtcgagttga
//
LOCUS       DBNHOH010000696         4044 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_234638, whole genome shotgun sequence.
ACCESSION   DBNHOH010000696 DBNHOH010000000
VERSION     DBNHOH010000696.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_234638"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="AC16V7_12100"
     CDS             complement(<1..415)
                     /locus_tag="AC16V7_12100"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR6257907.1"
                     /translation="MNGHLEIWIEAYLDDELSSLHRHQVEAHLGECQRCRALLEERKN
                     LSALLMALPDPANLKSEARFVAEVGLQLKQRRKALQIIPMVPDLGWYFIPVVLLLVLT
                     FFQTVAILSNMLWVVPGAKLILLDQVSFVSVLPSLP"
     gene            complement(412..975)
                     /locus_tag="AC16V7_12105"
     CDS             complement(412..975)
                     /locus_tag="AC16V7_12105"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR6257908.1"
                     /translation="MMMLEAVSVGDESKLISLAQEGDRQAFAELVSLYRLRVINMVYR
                     MCGDARVAEDAAQEAFIRVWQHLPDLRPGSSFRNWLYRIAVNSALDITRREKPNVDID
                     SLDLASPGEHLEVRLERRESAREIRQAVLSLPHASRVVLILREYEGLSYREIAATLDI
                     SIGTVMSRLHYARTRLVEILRPLMEAT"
     gene            complement(1311..1724)
                     /locus_tag="AC16V7_12110"
     CDS             complement(1311..1724)
                     /locus_tag="AC16V7_12110"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR6257909.1"
                     /translation="MDWNSRLEISQSEVQGRVPITILHVAGRINMSSTDILENAVRVA
                     YEKGARYFLLDLNKVPSLSSVGLRSIHWIYNILQSASQVGGEAKSGTAVGGTPVSPYV
                     KLLCSSPQVLKVLNMAGFDSYIEVYGELQEAISAF"
     gene            2265..2723
                     /locus_tag="AC16V7_12115"
     CDS             2265..2723
                     /locus_tag="AC16V7_12115"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6257910.1"
                     /translation="MNVGKSRQYTAGLNDLAEIRHFLQKRAAELQVDPSTTYDILLAV
                     TELVTNTMIYGYQNEPGFIEAEMYLEKDSLIIHLRDHAAPFNPTQFPPPDLSISLEKR
                     AIGGLGIFLAQQLVDQMIYRELPQGGNEVTLVIHQISTRQKEEGNDEDRN"
     gene            2707..3117
                     /locus_tag="AC16V7_12120"
     CDS             2707..3117
                     /locus_tag="AC16V7_12120"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR6257911.1"
                     /translation="MKIEIEFTKGKIPVTILSLQGDLDASNFESVIARARDLYTEGTR
                     HLLLDLSELRFMSSSGLVAMHSIVLLMRGTDPHDLLEGWNAIHAIDRDRASEPQSYVK
                     ILNPQPKVANTLKMTGLDQFFEIYQDRQKAIASF"
     gene            3120..>4044
                     /locus_tag="AC16V7_12125"
     CDS             3120..>4044
                     /locus_tag="AC16V7_12125"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR6257912.1"
                     /translation="MDTSLIRQVPLFSSLEPSGIELLAAALKECHYPAKTVLFKEGDY
                     GDKFYIVAEGEVAIIKAIGTLNERLLAIRGSGQFIGEMSLLNRDGLRTASARVHSDAR
                     LLELSREDFNALLDREPPLAYQMLSVLSQRLQTSQNQTIRDLSEKNRQLAEAYENLKA
                     AQEQIIEKKILERELMQAREIQESMLPTVLPRLKGFDIVAQMLPARMVGGDFFDVIRL
                     SPKSLGIVIGDVAGKGVPAALFMALTRSLLRAEAKPLVSPEIVLQNVNHTLLDMNAKG
                     LFVTVLYGILNQETREFNYVRAGHESPMLWDK"
BASE COUNT         1109 a         1060 c          945 g          930 t
ORIGIN      
        1 ctggtaaact cggcaatacc gagacgaaag atacctgatc taacaagatt aattttgcac
       61 ctggcaccac ccacaacatg ttgcttagaa tagccactgt ctggaaaaaa gtcaaaacca
      121 atagcagaac aaccgggatg aagtaccagc ctagatcggg caccatcggg atgatctgca
      181 gtgctttgcg gcgttgtttg agctgcaaac caacctccgc tacgaagcgg gcttcggatt
      241 taaggttagc cggatcaggg agtgccataa gaagcgcgga tagatttttg cgctcttcga
      301 gcagggcacg gcaacgctgg cattctccca ggtgcgcttc gacctgatgt ctatgcaggg
      361 aagaaagttc atcgtctaga taagcctcaa tccagatctc cagatgtccg ttcatgttgc
      421 ctccatcaat gggcgcagga tttccaccaa tcgtgtgcgg gcataatgca gtcgagacat
      481 aaccgtgccg atagagatgt ccaatgtcgc agcgatttca cgataggaaa ggccttcata
      541 ttcacgcaag atcaacacca cccggctggc atgtggcagg ctaagcaccg cttggcgaat
      601 ttcacgggca ctttctcgcc gttccaggcg gacttccaaa tgctctcctg gactggcaag
      661 gtccaagcta tcgatatcca catttggttt ttcacggcgg gtaatatcca gggccgaatt
      721 tacagcgatg cggtacagcc agtttcggaa ggaagagccg ggacgaagat caggcaggtg
      781 ttgccaaacc cggatgaaag cctcctgcgc tgcatcctca gcaaccctgg catcgccgca
      841 catccggtag accatgttga tcactctcaa gcgatagagg ctaaccaact cagcgaacgc
      901 ttggcggtcg ccttcctgcg cgagagatat cagcttgctt tcatccccca ccgaaacggc
      961 ttctagcatc atcatatctt tcccaaccca accaacccca ccattctatt taacgaattt
     1021 tatatcgatt ttattcggat tcagaaaaat aaaagttttt tgaaaaagcg cctggaaaat
     1081 ccccttatcc ttggctttgt tgcaaccact acctcaatca acagccatag tggctttccc
     1141 cacatcacat gcacttttgc aaattcgcgg cgcagatatt ttagcagtgg acaatatcac
     1201 ctaatccagg atgatggatt agcttcgctc gcagaaaaca aaatgggatc taagcaggtg
     1261 tttgtgcgcc cgcatcgatc ccaatataat caagttgtca ttagaacaaa tcagaacgcc
     1321 gagattgctt cctgaagctc tccgtagacc tcaatatatg aatcaaagcc agccatattg
     1381 agcactttga gcacctgcgg tgatgagcat agtagcttga cataaggcga aacaggagtt
     1441 ccgccaaccg ctgtgccaga cttagcttcg ccgcccacct gcgatgcact ttgcaagata
     1501 ttatagatcc agtgaatcga cctcagtccc acgctgctca gcgaagggac cttgttgagg
     1561 tcgagaagaa aatagcgagc tcccttttcg taagccacgc ggactgcgtt ctctaatata
     1621 tccgtactgc tcatgttgat ccgaccagcc acatgcagta tggtgatcgg aacgcgaccc
     1681 tgtacttcag attgcgaaat ttccaatcta ctgttccaat ccataatcca cattcctccc
     1741 tatccataaa tgcaacttgt attatggata agattctcac cttggtaatc attgttgaaa
     1801 agcgattata ggatacttga tatgcagcca ggaatcaaga gggaatcacc catttgcacc
     1861 gcaatcacgg gcagatcgac aaatactatc ccggttaccc agaacaggcc taatccaaag
     1921 gggagaaaaa catcgaccag ggagatataa tcatgaaaag tatactcaag attttcccat
     1981 taacttcgat aatttatcct acatacaaaa acatatcgtg ctcacagagt acattagggc
     2041 aatcgcattt gggcgattgc ccatcactga ggagagaaaa tggggtgttt tggtgggggc
     2101 atacccccac caaaacaccc caaacactct tttcacggga ttgcgcatac ccctcagggt
     2161 atatgcaatt gccctgcaga gtacatagaa aagactcgcc aacggcgaaa catctgatat
     2221 gattaatttt tgatagctac gagtgtacat tttcgctgaa gcttatgaat gttggtaaat
     2281 cacgccaata taccgccggg ttaaatgacc tagcagaaat ccgccatttt ctccaaaaac
     2341 gggcagccga acttcaggtt gatccttcta caacctacga tatcctcctg gcggtaaccg
     2401 agctggtcac caacacgatg atttacggct atcagaatga gccaggcttc atcgaagcag
     2461 agatgtattt agaaaaggat tcgctgatca tccacctgcg cgatcacgcg gcgcccttta
     2521 atccaacgca attcccaccg cctgatcttt ctatatccct tgagaaacgg gcgattgggg
     2581 gactgggtat ctttttagct caacaattag tagaccagat gatataccga gagttacctc
     2641 agggaggcaa cgaggttact ctggtgatcc atcaaattag cacaagacaa aaagaggaag
     2701 gaaacgatga agatagaaat tgaatttacc aaaggaaaaa tacccgtcac gatcctctct
     2761 cttcaaggag atcttgacgc ctctaacttc gaatctgtga tcgccagggc gcgcgatctt
     2821 tataccgagg gaactcgtca tttgttgcta gacttgagtg agctgcgctt catgagcagc
     2881 tccggcttgg tagccatgca tagcatcgtc ttgttgatgc gcggtaccga tcctcacgat
     2941 ctgctagaag ggtggaatgc aatccacgcc attgatcggg accgcgcgag cgagcctcaa
     3001 tcctatgtaa aaatcctcaa cccccaaccc aaagtagcca atactcttaa aatgacaggg
     3061 ctggaccagt tctttgaaat ctatcaagac cgacaaaaag cgatcgcatc attttagcaa
     3121 tggacacatc actcatccgc caagtgcccc tgttttcaag cctggagcca tctgggatcg
     3181 agctcctggc tgcagcgctc aaagaatgcc attatccagc caagacagtc ctttttaagg
     3241 agggagatta cggcgataaa ttctatatcg tcgccgaagg agaagtggcc atcatcaagg
     3301 ctatcggcac cctcaacgaa cgactgcttg caatacgtgg gagcggtcag ttcattggcg
     3361 agatgagcct gctcaaccgc gatgggctgc gcacagccag cgcccgcgtg cactcggatg
     3421 cccgcctgct tgagctgtcg cgtgaagatt tcaatgcctt gttagaccga gagccgccgc
     3481 tagcttacca gatgctgagc gtactcagcc agcgcctgca gacttcccag aaccaaacga
     3541 tccgcgatct ttcggaaaag aatcgccagt tggctgaggc ctacgaaaat ttaaaagccg
     3601 cccaagagca aatcatcgag aagaagatcc tggagcgcga gctaatgcag gcacgcgaga
     3661 tccaggaaag tatgctaccc acagttttac cgcgcctgaa aggcttcgac atcgtagcgc
     3721 aaatgctacc cgcgcgtatg gtgggtgggg atttcttcga tgtgatccgt ctcagcccaa
     3781 aaagtctcgg gatcgtcatc ggtgatgtgg ctggaaaagg tgtccctgcg gcgttgttca
     3841 tggcactcac acgcagtcta ttgcgagcag aagcaaagcc tttggtctca ccagaaatcg
     3901 tgctacagaa tgtgaaccat accctgttgg acatgaacgc caaagggctc ttcgtgaccg
     3961 tattgtacgg aatcctgaac caggaaaccc gcgagtttaa ttacgtgcgc gccgggcacg
     4021 agtcgcccat gctgtgggac aagc
//
LOCUS       DBNHOH010000697         1111 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_427000, whole genome shotgun sequence.
ACCESSION   DBNHOH010000697 DBNHOH010000000
VERSION     DBNHOH010000697.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_427000"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="AC16V7_12130"
     CDS             <1..573
                     /locus_tag="AC16V7_12130"
                     /inference="COORDINATES: protein motif:HMM:NF014679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR6257913.1"
                     /translation="SIHASSLLSGIFNAASVAAQPFSKQIHVIDSQYVTLGLGFQVLA
                     AAELALTDPLDNILTVIENVRQRIRVVALLDTLEYVRRSGRVSWARARIGDFLHIKPL
                     VEIRAGRILSLGQVRTRRNGIERLVDMLRNLGNLERLAILHTNAKEEAQRILDSQDLT
                     LSTNPFCVNVTPVIGAHTGPNALGFAAVVR"
     gene            592..>1111
                     /locus_tag="AC16V7_12135"
     CDS             592..>1111
                     /locus_tag="AC16V7_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257914.1"
                     /translation="MKPSLDKLHKFFKLEAERGYDNRAVMGGLERVLDNWEAEARSDN
                     LSEELIQSIRSQLRDYSRLDENARAEVLTRIWDRVQENSSSSLPTLPTPSKEEKTEST
                     PAKPAEPTPSKTDLPPHLHEPQHQPRVQINKSETASTVEAEVPAALNAPTTVLPNIGP
                     RHAQTLSRLGMHT"
BASE COUNT          293 a          311 c          268 g          239 t
ORIGIN      
        1 tctattcacg cctccagttt attgagtggt attttcaacg ccgccagtgt cgccgcgcaa
       61 cccttcagca aacaaattca cgtcatcgac agccagtatg tcacgctggg gctgggattc
      121 caagtattgg cagccgctga attggcttta actgaccccc tggataatat cctgaccgtt
      181 atagaaaacg ttcgccagcg gattcgtgtg gttgcactgc tagacacact cgaatatgtt
      241 cggcgtagcg ggcgcgtctc ctgggcgcgt gctcgcatcg gagatttttt acatatcaag
      301 ccactggttg aaatcagagc aggtcgtatt ctaagcctgg ggcaagtacg cacaaggcgc
      361 aatggaattg aacggctggt ggacatgcta cgcaaccttg gaaatctgga acgtctggca
      421 atcttgcata caaatgcaaa agaggaagcc cagcgaattc tagatagcca agaccttacc
      481 ctaagcacta accctttttg cgtcaacgtc acccctgtga tcggagccca tactggacca
      541 aatgcgctgg gatttgcagc cgtcgtcagg taactggtaa aatagcccct aatgaagcca
      601 tccttagata aattgcacaa atttttcaaa ttagaagccg aacgcggtta cgataaccgc
      661 gccgtgatgg gcggtttgga acgcgtgctc gataattggg aagccgaagc gcgtagcgac
      721 aacctttccg aagagttgat ccaatcgatc cgctcgcaac tgcgagacta ctctcgttta
      781 gacgaaaatg cacgcgcaga ggtcctgact agaatttggg atcgcgtcca agagaatagt
      841 agcagctctc tacccaccct gcctaccccg tcaaaagagg aaaaaacaga gtctaccccg
      901 gcaaagcctg cggaacctac cccctccaaa acagatctgc cacctcacct gcatgagccc
      961 cagcaccagc cgcgggttca aataaataaa tcggagacgg cttccacggt tgaagctgaa
     1021 gtacccgctg ccttgaacgc gcctaccacg gttttaccca atatcggacc tcgtcacgcc
     1081 cagaccctaa gcaggctggg aatgcatacc t
//
LOCUS       DBNHOH010000698         2744 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1196463, whole genome shotgun sequence.
ACCESSION   DBNHOH010000698 DBNHOH010000000
VERSION     DBNHOH010000698.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1196463"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..>2744)
                     /locus_tag="AC16V7_12140"
     CDS             complement(207..>2744)
                     /locus_tag="AC16V7_12140"
                     /inference="COORDINATES: protein motif:HMM:NF013256.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HIR6257915.1"
                     /translation="DRQAWELYWDLKIIADMALHLPENTPRSGQALFAANEMVNIIRL
                     DEKSTWTDAREVAARFLSAHNGDAQHNLSAIGHAHIDTAWLWPISETKRKCARSFASA
                     LCDVDAYPEYRFVLPQAAQLAWMKEDYPAVYARIKEKVRSGQFIPVGGTWIEPDCNIP
                     CGESLVRQFLYGQRFFESEFAMRCQEFWNPDVFGYSGALPQIMNLAGIQYFLTQKLSW
                     NQFNKPASHTFVWEGIDGSRVLTHFPPADTFNSMANVKEVLYNVSNFKDHERANESYL
                     LFGYGDGGGGPTPAMLEQLRRMVDVDGLPRAQIRSPREFFSRCAADIKDPLVMVGELY
                     FELHRGTYTSQARNKKYNRQSELLLRDVEILSCLAMVTRDHPYPATALQKLWEVVLTN
                     QFHDIIPGSSIGEVYQDSTQDYEFVLQKAGELRDVALKALSPEPPSANKVTVFNTLSF
                     PRTEVVELPEGIPGTQTTNTGRHLGIVTAPALGCAVQTPGNSDQPVAIIETAGGFVIE
                     NAHLRAVLTPSGGLISLFDKTAQRESIEQGQAGNNFVLYEDIPNAWDAWDVDIFHLEK
                     KLPLGGATEARVIESGPLRAAIEFTYAISPNSNMRQVVSLDAISARLDFFCDVDWHEK
                     HKFLKVEFPLNVRSQIATYEIQFGHVQRPTHFNTSQDMARFEVPAQRWADLSEPDYGV
                     ALLNDCKYGYAAHGNVLRLSLLRSPTHPDPDADQGRHQFRFALFPHAGSPQLAGVTEA
                     AYRFNVPLLICKSGEQDVHQSFFAVSNPAIIVDTLKKAEDSQAFILRLYESRGTRGAV
                     RLSSPLPFESAAVVNLLEDEISPLTWRDGGVDLDFKPFEIITIRLAR"
BASE COUNT          640 a          728 c          734 g          642 t
ORIGIN      
        1 gaacacgcag ggcaactgca tatgccctga ggggcatgcg caatcccgta aaaagagtgt
       61 ttggggtgtt ttggtggggg tatgccccca ccaaaacacc ccattttctc tcctcagtgg
      121 tgggaaatcg cccaaatgcg attgccctag tgaacacgct cttaagttga taaaaagcaa
      181 acatcgttgt cgtttatcta tacaaattat ctcgccaaac gaatagttat gatctcaaac
      241 ggtttgaaat ccaaatccac gccgccgtct cgccaggtga gcggtgaaat ttcatcctct
      301 aagagattga ccaccgctgc cgactcgaat ggcaaggggc ttgataatcg caccgccccc
      361 ctggtccccc gcgattcgta gaggcgcaag atgaatgctt ggctatcctc ggcctttttg
      421 agcgtatcca cgataatggc tggattgctt actgcgaaaa atgattgatg gacatcttgc
      481 tcgcccgact tgcatattaa aagcgggacg ttgaaccgat aggctgcctc tgtcactcca
      541 gccaactgcg gactcccggc gtgcgggaat aaggcgaaac ggaattggtg cctgccttgg
      601 tccgcatctg ggtcagggtg cgtaggggag cgcaggagtg agaggcgtag cacgtttcca
      661 tgcgctgcgt agccgtattt gcaatcattc agcaatgcta ccccgtaatc aggttcagac
      721 aaatctgccc atctctgcgc cgggacctca aagcgcgcca tgtcctggct tgtattgaaa
      781 tgcgtcgggc gttgtacatg cccaaactga atttcgtagg tggcgatctg tgaacgcacg
      841 ttgagcggaa actcaacttt caggaattta tgcttttcat gccagtccac gtcacagaaa
      901 aaatcaagcc gcgcagatat cgcgtccaac gagaccacct ggcgcatgtt gctattgggc
      961 gagatagcat acgtgaactc tatggcagcg cgcaagggac cggattcaat gaccctcgcc
     1021 tcagtcgcgc cacccaaggg taattttttc tctagatgga atatatccac atcccaggca
     1081 tcccaggcat tgggaatatc ctcgtaaagc acgaagttgt tgcctgcctg cccctgctcg
     1141 atgctctcac gctgagcggt tttatcaaac aggctgatca acccaccaga gggggtaaga
     1201 accgctctta aatgggcatt ctcaatgaca aacccaccag ctgtttcgat gatggcgacg
     1261 ggctggtcgc tgtttccagg ggtttgaacg gcgcacccca gcgcgggcgc agtgacgatg
     1321 cccagatgac gtcccgtgtt tgtcgtttgc gtgccgggga tcccttcggg gagctcgacc
     1381 acttcagtgc gtggaaaact gagcgtattg aagacagtta ctttattggc agatggtggt
     1441 tcaggtgaca gtgccttcag ggcaacgtct cgcagctcgc ccgctttttg cagcacaaat
     1501 tcgtaatcct gggtggaatc ctggtagacc tcgccaatcg atgaaccagg gatgatatcg
     1561 tgaaattggt ttgtgagcac aacctcccat agtttttgca atgcagttgc cgggtaggga
     1621 tggtctcgag tgaccattgc caggcaggag agaatctcca catcccttaa tagcagttca
     1681 gattggcggt tatatttctt atttcgcgcc tgagaggtgt acgtgccgcg gtgcaactca
     1741 aaatacaact cgcctaccat caccaatgga tcttttatgt ccgccgcaca acggctaaaa
     1801 aattcacgcg gcgatcgaat ttgagcgcgt ggcaagccat ccacatccac catgcggcgg
     1861 agctgctcca acatggcggg cgttggaccg ccgccgccgt cgccataccc aaagagcagg
     1921 taactttcgt tagcgcgctc atggtcttta aagttgctga cgttgtagag cacttctttg
     1981 acattggcca tagagttgaa ggtgtcggca ggggggaaat gggtcagcac acgcgagccg
     2041 tcgatacctt cccacacgaa ggtgtggctg gcaggcttgt taaactggtt ccaggaaagt
     2101 ttttgggtaa ggaaatattg gatccccgcc aggttcataa tttgcggaag cgcgccgcta
     2161 tagccaaaga cgtccgggtt ccagaattcc tggcaacgca tcgcaaattc cgattcaaag
     2221 aagcgttgtc cataaaggaa ctggcgcacg agtgattcac cgcatgggat gttgcaatcc
     2281 ggctcgatcc aggttccgcc cactggaata aattgtccgg agcgcacttt ttctttgata
     2341 cgcgcgtaca cagctgggta atcttccttc atccatgcca actgtgccgc ctgcggtagg
     2401 acaaaccggt attcaggata agcatccaca tcgcacagtg ccgatgcaaa cgaacgcgcg
     2461 cattttcgct tcgtctcgct aatcggccac agccaggcgg tatcgatgtg agcatgcccg
     2521 atagcagata aattgtgctg ggcatcaccg ttgtgcgctg aaaggaagcg ggcggcaacc
     2581 tcgcgggcat ctgtccaggt agatttctca tccaagcgga tgatgttcac catttcattc
     2641 gcggcaaata atgcctgccc gctgcgcggt gtattttccg gcaagtgaag cgccatatcg
     2701 gcaatgattt tgaggtccca atagagctcc caagcctggc ggtc
//
LOCUS       DBNHOH010000699         3778 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_169836, whole genome shotgun sequence.
ACCESSION   DBNHOH010000699 DBNHOH010000000
VERSION     DBNHOH010000699.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_169836"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..877
                     /locus_tag="AC16V7_12145"
     CDS             71..877
                     /locus_tag="AC16V7_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6257916.1"
                     /translation="MKKSSSVWAWFIFLIGIIYFFLPLFATFEFSMRMIKDTYSFEAY
                     RVAFTDPRFFLSFGYSLLWGILTIFVSLLLVVPTAYWVHWRMQRARPFVEFVTLMPFV
                     VPAVVLVFGMSSLYGGALKIGGFPILVLTPTPILLIAGYVVLSFPYMYRAVDTGLRAI
                     DVRTLTEAALSLGAGWRVILTQVIFPNLRVAILSGVFLTFAIVMGEFTFASLLVWPAF
                     GPYMEEIGAMRAYTPQALSVVSFILTWASIGLINWVGRGAPGSQQVAGAH"
     gene            895..1956
                     /locus_tag="AC16V7_12150"
     CDS             895..1956
                     /locus_tag="AC16V7_12150"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008124572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6257917.1"
                     /translation="MAFLELHNLSKSFGTTEAVSDFNLDIEKGELVSFLGPSGCGKTT
                     TLRMVAGFETPTTGHIKIDGMDITNNPPNQRDVGMVFQAYALFPNMTVANNIGFGLKI
                     AKKPPEEIKSRVEEMLSLIHMNGFGDRYPYQLSGGQQQRVALTRALALSPQVLLLDEP
                     LSALDAKIRVSLRSEIRAIQQQLGITAIYVTHDQEEALSLSDRVVVMNAGRMEQVGPP
                     FEIYNYPTTEFVAQFVGTLNAVSAEVVDPASQTLRLEGQLLQTEENLDGRRSGERVMI
                     AIRPERLSFAAEEKKANVIDCSIENITFLGSIVRIQVRLGNVSFSMDTFNNPYLELPK
                     IGDKTQITCSRKAVLILKQ"
     gene            1953..2762
                     /locus_tag="AC16V7_12155"
     CDS             1953..2762
                     /locus_tag="AC16V7_12155"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR6257918.1"
                     /translation="MNKVILIISDGLRYDTAVARMGYLGHLVEAKHASLYKVIGELPS
                     MSRPMYETIHTGLLTNEHGIVSNAVVRRSTKPNLFELAAKAGKTTAAAAYYWFSELYN
                     RAPFDPIEDREVDDKALLIQHGRFYTQDDYPDIDLFNTAALLVRRFQPDYLLIHPSGM
                     DYTGETYGSDSSQYRNHAIRQDVWLSKHIVEWLERDYSILVTGDHGISADRTHGGTTP
                     DMREVPLYMLGTGIQGRGDTGEVLSQLQVAPTICRLLDIPIPETMRATPIL"
     gene            2939..>3778
                     /locus_tag="AC16V7_12160"
     CDS             2939..>3778
                     /locus_tag="AC16V7_12160"
                     /inference="COORDINATES: protein motif:HMM:NF013543.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005315 - phosphate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HIR6257919.1"
                     /translation="MNLLIIIFVAIALLFDFLNGFHDSANVVATAIASRAMSPRFALG
                     MTALANFAGPFLFGVAVAKTIGNEVVGSEAVTVPVALAALISAIIWNVLTWLLGIPSS
                     SSHALIGGFVGAAVTGFGMSIVHLEGMVKVLIGLFISPLIGLVLGWFVMRQSLFVGRH
                     SPPSINLFFRRAQWLTALTLGLSHGTNDAQKTMGIIALGLVAFGVLPEFRVPFWVIAA
                     SAGAIALGTALGGWRLIRTMGAGFYRIRAIHALSSQVASTIVILGAAILGAPVSTTQV
                     ISST"
BASE COUNT          771 a         1112 c         1009 g          886 t
ORIGIN      
        1 gatgggtgcg atgagagatg attctaaata gacgtaaaga tcacgccgat ccaaggcgaa
       61 aggaacgact atgaaaaaga gttcaagcgt ctgggcttgg ttcattttct tgatcggtat
      121 catctacttc ttcttgccgc tcttcgctac cttcgagttc agcatgcgga tgattaaaga
      181 cacctattct tttgaagcct accgggtagc ttttacagac ccgagatttt tcctcagttt
      241 cggctactct ctcctgtggg ggatcctgac gattttcgtc agcctattgc tggtcgtacc
      301 gactgcttat tgggtgcact ggcgcatgca acgcgcccga ccgtttgtcg agttcgtcac
      361 cctgatgcct ttcgtggtgc cggccgtagt cctcgttttt ggcatgagca gcctgtacgg
      421 tggcgcgctg aaaattggcg gcttccccat cctggtgctg acccccaccc ccatcctact
      481 gatcgccggg tatgtggttt tatctttccc ttatatgtat cgcgcggtag ataccggctt
      541 gcgcgccatt gacgtgcgca ccctcaccga ggctgccttg agcctggggg ctggctggcg
      601 ggtcatcctg acgcaagtca tcttccccaa cctgcgcgtg gccatcctga gcggcgtttt
      661 tctgaccttc gccatcgtga tgggcgagtt caccttcgcc tcgctgcttg tctggcctgc
      721 ttttggccct tatatggaag agatcggcgc catgcgagcc tacacgccgc aagcgctgtc
      781 agtcgtcagc tttattctca cctgggcatc catcggcctg atcaactggg ttgggcgcgg
      841 cgcccccggc tcacagcaag tcgccggagc acactaataa gaagaggtca taacatggca
      901 tttctggaat tgcacaacct gagcaaatcc ttcggcacaa ccgaggcggt ctctgatttc
      961 aatctggaca tcgaaaaagg cgagctggtc tctttcttgg gtcccagcgg ctgcggaaaa
     1021 accaccacac tgcgcatggt ggctggcttt gagactccca ccaccggtca tattaagatt
     1081 gatggcatgg acatcaccaa caaccccccc aaccagcgcg atgtcggcat ggtatttcag
     1141 gcctacgccc tatttccaaa catgaccgta gccaacaaca tcggctttgg cctaaaaatc
     1201 gctaagaaac cgccggagga gattaagtcc cgggtagaag agatgctctc tttgatccat
     1261 atgaacggtt tcggtgaccg ctacccctac cagctctccg gcgggcagca gcagcgcgtc
     1321 gccctgacgc gtgccctggc attaagtcct caggtgctgt tgctggatga gccgctttcc
     1381 gccttagatg ccaagatccg cgtctcgctg cgctccgaga tccgcgccat ccagcagcaa
     1441 ctgggcatca ccgccattta cgtgacccac gaccaggaag aggctctatc gctatccgac
     1501 cgggtggtgg tgatgaacgc ggggcgcatg gaacaggtgg ggcctccctt tgagatctac
     1561 aactacccca ccactgaatt cgtcgcccaa ttcgtgggaa ctctgaacgc tgtcagcgct
     1621 gaggtggttg acccagcttc tcagacatta aggctcgagg ggcaactgtt acaaaccgag
     1681 gaaaacttgg atggacggcg ttctggtgag cgggtgatga tcgccatccg ccccgagcgc
     1741 ctgagttttg ccgccgaaga gaagaaggcc aacgtcattg attgcagcat cgagaacatt
     1801 acctttttgg gctctatcgt acgcatccag gtgcgcttgg ggaacgtcag cttctctatg
     1861 gacacattca acaacccgta cctggagctg cccaagatcg gggataaaac acagatcacc
     1921 tgctcgcgca aggccgttct gatcctaaag cagtgaacaa agttatcctg atcatttccg
     1981 atgggctgcg atatgatacg gccgtcgcca ggatgggcta tttaggacac ctggtggaag
     2041 ccaagcacgc cagcctgtat aaagtaattg gcgagctgcc cagcatgtcc cgtcccatgt
     2101 atgagaccat ccacactggc ctgctcacca atgagcatgg catcgtctcc aatgctgtag
     2161 tgcgccgctc gacgaaacct aacctctttg agctggcagc aaaggccggg aagaccactg
     2221 cggcagccgc ctactactgg ttctccgaac tgtataaccg cgcccctttt gaccccatcg
     2281 aagaccgcga ggtagacgac aaggcgctgc tcattcagca cgggcgcttc tatacccagg
     2341 acgattaccc agatatcgac ttattcaata ccgcggcact gctggtgcgc cgcttccagc
     2401 cagattacct gctgatccac cctagtggca tggattatac tggtgagact tatggatccg
     2461 attccagtca gtaccgcaat cacgccatcc gccaagacgt gtggctgtca aaacacatcg
     2521 tggaatggct ggaacgtgat tactcgatct tagttaccgg cgatcatggg atcagcgctg
     2581 accgcaccca tggcgggacc acgccagaca tgcgcgaggt gccgctttac atgttgggta
     2641 cgggtatcca aggcaggggc gacacagggg aagtgctctc ccagttgcag gttgccccca
     2701 cgatatgccg gttgctggat attcccatcc cagaaaccat gagggccacg ccaatcctct
     2761 aagccgtgcc cgcatacatg taggaaaagt gtcattattg acgttctcag ccaccaagat
     2821 gacctcttca caatatctgt gaaggcctag atggcacaaa gaacgctacc tggtaccaga
     2881 agacatcaat tctcaaacag atcacggggt ggctgtccaa ggtaaggagt ctgtatcgtt
     2941 gaaccttctc atcattatct tcgtagccat agctttgcta tttgattttc tgaatggttt
     3001 ccatgacagt gccaatgtgg tcgccacggc cattgcctca cgagccatgt cgccgcgctt
     3061 cgccttgggc atgaccgccc tggcaaactt tgctgggccg ttccttttcg gcgtggcggt
     3121 agccaaaacc atcggcaatg aagtcgtcgg ctccgaggcg gtcactgtgc ccgtggcgct
     3181 cgccgccctg atctctgcca tcatctggaa cgtgctcacc tggttgctgg gcatcccctc
     3241 cagttcctcg catgcgctga tcggcggctt cgtaggcgcg gcagttaccg gctttggcat
     3301 gagtatcgtg catctggaag gcatggttaa agtcttaatc ggtttattta tctccccgct
     3361 gattggcctg gtcctcggct ggtttgtcat gcgccaatcc ctcttcgttg ggagacattc
     3421 acccccaagc atcaatttat ttttccggcg cgcccagtgg ctcactgccc tgaccttggg
     3481 gctgagtcac ggcaccaacg atgcccagaa gaccatgggc atcatcgctt tgggtttggt
     3541 ggccttcggc gtcttgcccg agtttcgtgt cccattttgg gtgatcgcag ccagcgcggg
     3601 cgccattgcc ttggggacag ccttaggtgg ctggcggctg atccgcacca tgggtgccgg
     3661 cttctaccgc attcgcgcca ttcacgcgct gtcttcacag gtcgccagca caatcgttat
     3721 cctgggtgcg gcgatcttgg gtgcgccggt gagcaccacc caggtgatca gctcaacc
//
LOCUS       DBNHOH010000700         5268 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1324693, whole genome shotgun sequence.
ACCESSION   DBNHOH010000700 DBNHOH010000000
VERSION     DBNHOH010000700.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5268)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5268
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1324693"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="AC16V7_12165"
     CDS             <1..305
                     /locus_tag="AC16V7_12165"
                     /inference="COORDINATES: protein motif:HMM:NF012383.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HIR6257920.1"
                     /translation="RGYNQAALLARPIALRFGVNYCPKALIKTRETRSQVGLNVEERR
                     KNVHGAFKSDQQVVAGKRVLVVDDVTTSGATLNACADALRTAGAMGIYCLTLARAL"
     gene            418..990
                     /gene="hpf"
                     /locus_tag="AC16V7_12170"
     CDS             418..990
                     /gene="hpf"
                     /locus_tag="AC16V7_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HIR6257921.1"
                     /translation="MSMDVQVYGRNMDVTDRIRTYVSKKISKLDRYLSGIEEARVDVA
                     YVKSARSASDRQVAQITVRGKGFILRSEERSDDIITALDIALDKMQRRIERYKGKRYR
                     GRGDGRSAAEVVPQVVEEIEEESGELGPMIARRKRFTLHPMDEMEALEQMQLLGHENF
                     FVFYNANTYSVNVLYKRRDGNYGLIEPDVG"
     gene            complement(1092..1301)
                     /locus_tag="AC16V7_12175"
     CDS             complement(1092..1301)
                     /locus_tag="AC16V7_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257922.1"
                     /translation="MTVYKVSYVVTGSEHPGAIVNLDHAPAVGEHITLGDHKFEVIEV
                     LNLVPPRGDFHYIHATIKLVATFQD"
     gene            complement(1305..1751)
                     /locus_tag="AC16V7_12180"
     CDS             complement(1305..1751)
                     /locus_tag="AC16V7_12180"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR6257923.1"
                     /translation="MELTNILRKAELFNGLSEDELQHVASLCGERRLARGEILTTQGV
                     RGDELFIVTEGLVEVLITETQKDSHKIIVNLGKGQLIGEMSLVDKGPRSATVRAITEP
                     TLVQVIQRDQFEALCEQNTHIGYIVMRNIATDLSFKLRHRNLSEGK"
     gene            1935..2567
                     /gene="folE"
                     /locus_tag="AC16V7_12185"
     CDS             1935..2567
                     /gene="folE"
                     /locus_tag="AC16V7_12185"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019010402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HIR6257924.1"
                     /translation="MDTQFELLYKIADLRQDGEIDHDSIKDAVIQLLQAIGEDPDREG
                     LIRTPERVSRMYAELLDGYRMDPIEIVNDALFEVMYDEMVLVRDIEFYSLCEHHMLPF
                     FGRAHVAYLPKGRVLGLSKIPRLVDMFAHRLQVQERMTRQIAEFLDELLHPAGVAVVV
                     EGLHLCTAMRGVKKHDARMTTSAMLGYFRKNIATRQEFLDNISRGAAPMF"
     gene            complement(2609..3094)
                     /gene="folK"
                     /locus_tag="AC16V7_12190"
     CDS             complement(2609..3094)
                     /gene="folK"
                     /locus_tag="AC16V7_12190"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:NF013456.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIR6257925.1"
                     /translation="MDEHQYILGLGSNIDPVTNLHRTVEMLRQEVTVDNISTIWVTKP
                     VGEAGPDFLNAAVQIKSTLEPNTLKSQVLRPIEAKLGRIRTKQKNSPRTIDIDILFAD
                     DQIVDDEVWEYTHKALPLAELKPDLIHPTTGETLEILARRLLRSTPLDHRPAILKLNK
                     I"
     gene            complement(3087..3443)
                     /gene="folB"
                     /locus_tag="AC16V7_12195"
     CDS             complement(3087..3443)
                     /gene="folB"
                     /locus_tag="AC16V7_12195"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HIR6257926.1"
                     /translation="MDKIIIKDLLARGVIGVNEWERQVTQDILMNIVIFSDVRKAGET
                     DDLTYSVDYRKIAKQAVVLAETAKRLTVEALASDIASMCLEDAGVLKVQVRVEKPGAI
                     RFARSVGVEIERSRDG"
     gene            complement(3519..4244)
                     /locus_tag="AC16V7_12200"
     CDS             complement(3519..4244)
                     /locus_tag="AC16V7_12200"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6257927.1"
                     /translation="MSLSGKHVLITGSARRVGRILALSVAQAGGIVFIHHAHSPEDAE
                     NVRQEIIDLGGQAYILQADLLDPSMAQKLIDQASTYGPLHALVNNAAIFEPLSWETTD
                     LESWNRHLALNLSAPFLLSQAFARSHTELTVGRIVNILDWRALRPGPDHFPYTISKAA
                     LVALTRSLAIALSPQITVNGIALGAILPPTHPVDLRKVIQHVPAGRWADPEEVGDALI
                     FLLEGPSYITGEIIHLDGGRHLV"
     gene            complement(4241..4651)
                     /locus_tag="AC16V7_12205"
     CDS             complement(4241..4651)
                     /locus_tag="AC16V7_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HIR6257928.1"
                     /translation="MSSQLSFYIPLNISYDEALEAVVKALSAEGFGILTRIDVKATLK
                     EKIGVEFRPYTILGACNPPLAHRALSSDPETGMLLPCNVTVETSEAGGSIIRIVNPQT
                     LLSIGSLSEDPELAAVAKEAHTRLERVAQTLKQT"
     gene            complement(4661..>5268)
                     /locus_tag="AC16V7_12210"
     CDS             complement(4661..>5268)
                     /locus_tag="AC16V7_12210"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR6257929.1"
                     /translation="NLALTGLVAGLVAARLGYVLRYPYAFSENIMDVFSRNLGLFDTQ
                     TGFVVGLIASAIYAQRKQISLKATLDALTPAFAVFSIALSLSHIASGDAYGSPATLPW
                     SIQLWGAQRHPSQIYETLAGIAILGYLWPSHKHLANLKPGEYFLKFVIMNSAARLFLE
                     AFRGDSAVTLGGYRAAQIISWLILAVCLYAIKRLRTTQGPP"
BASE COUNT         1373 a         1149 c         1325 g         1421 t
ORIGIN      
        1 agcgaggata taatcaagca gcactattgg ctcgaccgat agcattgagg tttggtgtca
       61 attattgtcc aaaggcctta atcaaaacca gagagacgcg ctctcaggta ggtctcaatg
      121 tggaagagcg acgcaagaac gttcatggag cattcaaatc tgatcagcag gtggtagctg
      181 ggaagcgagt ccttgttgtg gacgacgtaa cgacgagtgg ggctaccttg aacgcttgtg
      241 ccgacgcttt gaggactgct ggagctatgg gaatttattg ccttacactg gcgcgggctt
      301 tataattaga tatggagtac aactgctaac cattcttatg atatgtttca gttattataa
      361 ggtttattta gtggcgagca ttgaatggca ttttatacga tgatatagga ggaaaaaatg
      421 tccatggatg tacaagttta tggtaggaat atggatgtca cggatcgcat ccgaacttat
      481 gttagtaaaa agatttcgaa attagatcga tacttatctg ggattgagga ggcaagggtt
      541 gacgtcgctt atgtgaaatc ggctcgcagc gcgtctgatc gacaagtggc acaaattaca
      601 gtccgcggca aaggatttat attgcgctcg gaagagcgtt ctgacgacat catcaccgct
      661 cttgatatcg ctttggataa aatgcaacgc aggattgagc gatataaagg caagcgctac
      721 agagggcgtg gtgacgggag gagtgccgct gaggttgttc cccaggtagt tgaagagatc
      781 gaagaagaaa gtggtgaatt ggggccgatg attgcccgca ggaagcgatt tactctccac
      841 cccatggatg agatggaagc ccttgagcaa atgcaattgc taggacacga gaacttcttc
      901 gttttctaca atgcgaatac ctattcggtc aatgtgcttt ataaaaggcg agatggcaat
      961 tatggcctga tcgaacccga tgttggctga ttgtatgcaa tatctagcca aagtttgagc
     1021 aaatctcaat atttcaaaat cccccttccg agtagaaggg ggattgattt tagtaattct
     1081 tctttgggaa atcaatcttg gaaagttgct actagcttga ttgtagcgtg aatgtaatga
     1141 aaatcgccac gcgggggcac aagattcaac acttcgataa cctcaaattt gtgatccccc
     1201 agggtgatgt gttcgccaac tgcgggagca tggtcgagat tcacaatggc tccagggtgc
     1261 tcactacccg tgaccacata gctaacttta tagacggtca tcacctactt tccttcactc
     1321 agattgcggt gacgtagttt gaaagagaga tctgtggcaa tatttcgcat cacaatatat
     1381 ccgatgtggg tattctgctc acataaagct tcaaactgat ccctctggat aacctgaact
     1441 aaagtaggtt cagtaatggc tcggacagtt gccgagcgag gccctttatc aaccaatgac
     1501 atttcgccga tgagctgccc cttacctaag ttgacgatta ttttatgaga atccttttga
     1561 gtctctgtga tgagcacctc aaccaaacct tcggtgacga taaatagctc gtcgccccga
     1621 accccttgtg ttgtaagaat ctcacctcgc gccaagcgtc tctccccaca taatgatgcg
     1681 acgtgttgga gctcgtcttc cgaaagtccg ttaaaaagtt ctgccttacg caggatgttt
     1741 gtcagttcca tcaatctgcc tctcactgat agaatcattt tataacgaat ttttcttaac
     1801 tgcaacattt tcgtatttgt ttgtagagaa aggctctact tcatgcagtt tcctttgtat
     1861 gcctccgcgt tgacgcttgg cgatgagagg tgttacacta ggcgcaaaat tatataagaa
     1921 tgctaaggat cattatggat acacaatttg agctgctgta taagattgct gatctgcgtc
     1981 aggatggaga aatagaccat gattcaatta aggacgcggt aattcaattg ttacaagcaa
     2041 ttggggaaga tcccgaccgg gagggtttaa ttcgcacacc tgagcgggta tcccgcatgt
     2101 atgctgagtt actagatggt taccgtatgg accccatcga aattgtcaat gatgctttgt
     2161 ttgaagtgat gtatgatgaa atggtgctgg ttcgcgacat cgaattctat agtctctgcg
     2221 agcaccatat gcttccattt tttggaagag cacatgtagc ttacttgcca aagggaagag
     2281 tattgggtct gtcaaaaata cctcgattgg tagacatgtt cgcccacagg ttacaggttc
     2341 aagagcgaat gacccggcag attgctgaat ttttagacga attgctccat ccagcagggg
     2401 tcgcggtagt tgttgaaggt cttcatcttt gtacagcaat gcgtggggtt aagaaacacg
     2461 atgcccgcat gacaacctcg gctatgttag gttattttcg taaaaatata gccactcgac
     2521 aggagttctt ggataatata tcacgcgggg ctgcgccaat gttttagtcc ccatctataa
     2581 attggctgaa gagttcccca attgagattt aaatcttatt gagtttaaga attgccggac
     2641 ggtgatcgag tggcgtggag cgcaataacc tgcgagccaa gatttccaaa gtttcccctg
     2701 tagttggatg gatcaggtca ggtttcaatt cagccagggg aagggctttg tgtgtatatt
     2761 cccacacctc atcatcaaca atttgatcat ctgcgaacag aatatctatg tcgattgtgc
     2821 ggggggaatt tttttgtttt gttcttatcc gccctagctt ggcttcgata gggcggagga
     2881 cctgcgactt taatgtatta ggttccaggg tagattttat ttgaacagct gcattaagaa
     2941 aatctggccc tgcctcgcca actggtttgg tcacccaaat ggtggaaata ttatcgacgg
     3001 ttacctcctg acggagcatt tctacagttc gatgcaggtt cgttactgga tcaatattgg
     3061 acccgagacc taagatatat tgatgctcat ccatctctac tacgttcaat ttcaacgccc
     3121 actgagcgtg caaaacgtat cgcgcctggc ttttctaccc gaacttgaac tttcaataca
     3181 ccagcatctt ctagacacat acttgcgatg tctgaagcca gagcttcaac ggtcaaccgt
     3241 ttagcagtct cagctagaac aacggcctgc ttggcaattt ttcgataatc tacgctatag
     3301 gtaagatcgt cggtttcgcc tgccttgcgt acgtcgctga atattacgat gttcatcagt
     3361 atatcctggg tgacttggcg ctcccactca ttcacaccaa tcacaccccg agctaacagg
     3421 tcttttatga ttatcttatc cataatgacg ccttcctggt ttcacgaaaa gtggctctga
     3481 tacttagtca gattttccgt tggtagaaag tatgtgctct atacgaggtg ccgcccacca
     3541 tccaagtgga taatttcacc cgtgatgtag ctcggtcctt ccagaagaaa aatcagcgcg
     3601 tcaccgactt cctctgggtc tgcccatcga ccagcgggta catgctggat gacttttctt
     3661 aagtcaactg gatgagtggg ggggagtatc gcgcctaaag caatcccgtt cacagtgatc
     3721 tgcggggata aggctattgc tagggatcga gtgagggcta ctaaggctgc tttgctgatg
     3781 gtgtatggga agtggtctgg cccagggcgc agggcgcgcc aatctaagat attgacaata
     3841 cgacctacgg tcagctccgt gtgcgagcgt gcaaaagcct ggctgaggag aaatggggcg
     3901 gataaattga gggccaggtg tcggttccag gattcaagat ctgtggtctc ccacgaaagt
     3961 ggttcaaaga tggccgcatt attcaccaaa gcatgcaatg gaccgtaggt actcgcttga
     4021 tcaattaatt tctgtgccat tgacggatca agcaggtccg cctgtagtat atatgcctgt
     4081 ccacccaggt cgataatctc ctggcgtaca ttttcggcat cttcaggaga gtgagcatga
     4141 tggatgaata caatccctcc agcttgcgca actgatagag ccaggatacg cccaacacgg
     4201 cgggctgaac cggtaattaa aacatgcttg ccagatagac tcacgtctgt ttaagtgttt
     4261 gggcgacgcg ctccaggcga gtgtgagctt ctttggcgac tgcggctaat tcggggtcct
     4321 cagagagcga cccgattgat agcagagttt ggggattcac gatgcggata atagacccac
     4381 ccgcttcgct ggtttctacg gtgacattgc acggaagtag catccctgtc tcgggatcgg
     4441 atgagagagc tcggtgggct aatggggggt tgcaagctcc tagtattgta tatgggcgaa
     4501 actcgacgcc gatcttttcc ttcaatgtcg ccttgacatc aatgcgagtc aggataccaa
     4561 atccttctgc acttagcgct ttgacaaccg cctcgagagc ttcgtcatat gagatattta
     4621 ggggaatata aaagctaagc tgagaagaca tgtgttctcc ttatggtgga ccttgagtag
     4681 ttcttaagcg cttgattgcg tatagacaaa cggccaggat tagccaacta atgatctgcg
     4741 cagctcgata cccgcccagg gtaacggcgc tgtcgccacg aaaggcttct agaaataatc
     4801 gagctgcgga gttcattata acgaatttaa ggaaatattc accgggtttt aggtttgcga
     4861 gatgtttgtg ggatggccac agatatccta gtatggcgat tccagcgagc gtttcgtaaa
     4921 tttggctcgg atggcgttga gctccccata attgaatgct ccaaggtaaa gttgctggcg
     4981 agccataagc atctcctgaa gctatgtggg aaaggctgag tgcaatgctg aaaacagcaa
     5041 atgccggagt aagtgcatca agcgtggctt tcaatgaaat ttgcttgcgt tgtgcataga
     5101 tcgcggatgc gatcagcccc acgacaaagc cagtttgtgt atcaaacaat cccagattgc
     5161 gagaaaaaac atccataata ttttctgaaa aagcatacgg atagcgtaaa acatatccca
     5221 aacgcgccgc aaccaagcca gcaactaagc ctgtgagagc caggttga
//
LOCUS       DBNHOH010000701         3384 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1517045, whole genome shotgun sequence.
ACCESSION   DBNHOH010000701 DBNHOH010000000
VERSION     DBNHOH010000701.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1517045"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..1537
                     /locus_tag="AC16V7_12215"
     CDS             80..1537
                     /locus_tag="AC16V7_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004556619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HIR6257930.1"
                     /translation="MDFSALGTSLSNSLGAHLPNILGALVILIVGWVAAVVARAALIR
                     LLAMLKVNQRIQESTDQKMNVESGIAVGVFWVILLVTVLAVLNALDLKLVSDPFQSMV
                     TQILSYLPRLAAGTILVLIAWLVATVLRKLVTRVLEQSGVDKKISAKTGMVPMSKNVG
                     NVLFWLVILLFIPAILGAYALEGLLAPVQSMINKSLAVLPNVFAAVVIGFVGWLVAKV
                     LRGLVVNLLAAAGADKAGHKAGLEPSVQISRVAGTLVFIFVFIPTLIAALDALQIEAV
                     SRPATNMLARILDAVPQLVAAGLILVVTWYVAKFASSLVTRLLNSMGLDSVPEKIGLG
                     GTFGGEFKPSTLIGILIMFFAMLFATVEAANQLQFTQVRDVITIFINFGGNILLGAVI
                     LLIGFWLANLAYLGITRTGGKGSNPLASVARVAILGLVIAMGLRAMGIADDIVNVAFA
                     LTFGAVAVAVALSFGLGGREAAGKQMEHWLSKLRK"
     gene            1613..2179
                     /locus_tag="AC16V7_12220"
     CDS             1613..2179
                     /locus_tag="AC16V7_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257931.1"
                     /translation="MQSQAIAGNNFLTGLCNSFPLASRRAGYDGEIAKGRAEHLDHRG
                     KHCSGNCLPATIQRGSIVNMKQLLIGIVLLLAGGGALSAEPQRYPDVVSARVQQRAPD
                     IFDFDVTVSSPYDTPQRYADAFRVMGKDGVVFGERKLLHDHAHEQPFTRDLYGVRIPA
                     GIRVVVIQARDQKYKYGGKTVEVVLPGR"
     gene            2226..2930
                     /locus_tag="AC16V7_12225"
     CDS             2226..2930
                     /locus_tag="AC16V7_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR6257932.1"
                     /translation="MIELYYWPTPNGHKITMFLEEAGLPYEIKPININAGDQFKPEFL
                     KISPNNKIPAIIDHAPADGGAPVSVFESGAILLYLAEKTGRFLPADARDRLTVIEWLF
                     WQVSGLGPMAGQIHHFGLYAPDKIPYAIKRYTNETNRLYGVLDNQLAESDFIAGTDYS
                     IADIATYPWIVPQKQGQNLDDFPHLKLWHEAVAIRPGTIRAYEKAKPWMNQSAVTEVG
                     TKILFGQTAANVTRYA"
     gene            2986..3378
                     /locus_tag="AC16V7_12230"
     CDS             2986..3378
                     /locus_tag="AC16V7_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257933.1"
                     /translation="MNRIFLILFLVFSVNSFAASLQPADSKLIKAAGVPLFSKATFVY
                     GNKDVGFRFASSTTPEEVQKWYRQQLPKWALLKEYGGWILYNGAPGVGMGEVMSKNQV
                     SVKHNDNLPQWHSLDKKMTTEIVIMIVN"
BASE COUNT          731 a          918 c          926 g          809 t
ORIGIN      
        1 agctaatata tccgtttcga tcagatagtg agtgctttat aattgatcgg atatataaat
       61 cgttaataat ggaggcacca tggacttctc agcacttggt acatcattgt ccaattccct
      121 cggagcacat ctgcccaata tccttggcgc cctggtgatc ctgattgtcg gctgggtggc
      181 agcggttgtt gcccgcgccg cgttgatccg tctgctggcg atgctgaaag tgaaccagcg
      241 cattcaagag agcaccgatc agaaaatgaa cgtcgagtcc ggcatcgccg ttggcgtatt
      301 ctgggtgatc ctgctggtga ccgtgctcgc cgtgctgaat gccctcgacc tcaagctcgt
      361 ctccgatccg ttccaatcca tggtgaccca gatcttaagc tacttgccaa ggctggcggc
      421 cggcacaatc ctggttctca tcgcctggct tgtcgccacg gtgttgcgca agctggtcac
      481 gcgcgtactg gagcaatcgg gagtcgataa aaaaatcagc gctaaaacgg gcatggtgcc
      541 gatgagcaaa aatgtcggca atgtactatt ctggctggtg atcctgctgt tcattcccgc
      601 aattctcggc gcctacgcgc tcgaaggact gctcgcgccg gtacagagca tgatcaacaa
      661 gtcgctcgcc gtgcttccca acgtttttgc tgccgtggtc atcggtttcg ttggttggct
      721 cgtcgcaaag gtgctacgcg gcctggtagt caacctgctg gccgcagcag gcgcggacaa
      781 ggccggccac aaggcaggac tcgagccgtc ggttcagatt tctcgcgtgg cggggactct
      841 ggtgttcatc tttgtgttca tcccgacgct gatcgccgct cttgatgcgc tgcagatcga
      901 agcggtatca cggccagcga ccaatatgtt ggcgcggatc ctcgatgcgg taccacaatt
      961 ggtggccgcc ggcctgatac tggtcgttac ctggtacgtg gccaagtttg cctcgtcgct
     1021 tgtgacgcgg ctgttgaaca gcatggggct tgattcagtc cccgagaaga ttgggctcgg
     1081 tggtactttc ggcggcgaat ttaagccttc gaccctgatc ggcatactga tcatgttctt
     1141 cgccatgctc ttcgctactg tggaggcagc gaaccagctt cagttcacgc aggtacgcga
     1201 tgtcatcacc attttcatca acttcggagg caacatcctg ctcggtgccg tgatcctgct
     1261 tatcgggttt tggctcgcaa acctcgccta cctggggatt acccgtaccg gcggcaaagg
     1321 cagcaatccg ctagcgagcg tcgctcgcgt cgccatcctt ggcttggtga tcgccatggg
     1381 cttgcgtgcg atgggtattg ctgacgatat cgtcaatgtt gcgttcgcgc ttaccttcgg
     1441 tgccgtcgcc gttgccgtgg cgctttcctt tggcctcggc ggccgcgaag cggccggcaa
     1501 gcagatggag cactggctga gcaagctgcg caagtgacat tcatctaagc aggcgcaacc
     1561 accttcacct gcggaatcag cgggtagagc gggttgcgta tcgagggcta gcgtgcagag
     1621 tcaggcaatc gccggcaaca attttcttac tggtttgtgc aactcattcc ccctcgcatc
     1681 tcgccgcgcc ggctacgatg gcgagatagc gaaggggaga gccgagcacc tggatcaccg
     1741 aggcaagcac tgctctggga actgcttacc tgccactatt cagcgcggga gtatcgtgaa
     1801 tatgaagcag ctattgatcg gcatcgttct gcttctggca ggaggcgggg ccttgtcggc
     1861 cgaaccacag cgttaccccg atgttgtttc tgccagggtc caacagcgcg cccccgatat
     1921 ttttgacttc gacgtcacgg tttcttctcc atacgacaca ccacaaagat acgccgatgc
     1981 ttttcgcgtc atgggcaagg atggcgtggt gttcggtgag cgcaaactgc ttcacgacca
     2041 tgcccacgaa caacccttca cccgagatct ctatggcgtc agaatcccgg ccggaatacg
     2101 cgtagtcgtt atccaggccc gagatcagaa gtataagtat ggtggtaaga cagttgaagt
     2161 tgtcttgccc gggcgctaat tcgggagaac agacggggca ggctcgataa ttattgaggt
     2221 gacgcatgat cgaactttat tactggccga cgcccaatgg gcataaaatc acgatgtttc
     2281 tggaggaggc cggccttcct tacgagatca agcccatcaa cattaatgcc ggggatcagt
     2341 tcaagcccga gtttctgaag ataagtccca acaacaagat cccggcgatt atcgatcacg
     2401 cgccagccga cggcggcgcg ccagtctcgg tattcgaatc cggcgccatt ctcctttatc
     2461 tcgccgagaa aaccgggcgc tttctccccg ctgatgcacg tgaccgcctc actgtaatcg
     2521 agtggttgtt ctggcaggtc agcgggctcg ggcccatggc cgggcagatt catcatttcg
     2581 ggctatatgc gccggataag atcccctatg ccattaagcg ctacaccaat gagacgaacc
     2641 ggctctatgg cgtgcttgat aatcagttgg cggaatctga cttcatcgcg ggaactgatt
     2701 acagcattgc cgatatcgct acttacccct ggatcgtgcc gcaaaagcag ggacagaacc
     2761 tggatgactt tcctcacctc aaactgtggc atgaagccgt cgccatccgt cccggcacca
     2821 tccgtgctta tgagaaagcc aagccgtgga tgaaccaatc agccgtcacg gaagtaggca
     2881 cgaagatcct cttcggccaa accgccgcga acgtcacgcg ttacgcttga gataaccgcc
     2941 gtattcatat gttcaaatga tttgctaaat gaataggaga gagaaatgaa tagaatattt
     3001 ctcatattgt ttttagtttt ttcagtgaat tcgtttgcag cgagtcttca acccgccgat
     3061 tccaaattga tcaaggctgc cggtgtcccg ctcttttcta aggctacctt cgtctatggg
     3121 aacaaggatg ttggttttcg ttttgcctcc agcacaacac cggaagaagt tcaaaaatgg
     3181 tatcgccagc agttgcccaa gtgggcatta ttaaaagaat atggtggttg gattctctat
     3241 aatggagcgc caggcgtggg catgggggag gtgatgtcca aaaatcaggt cagcgttaaa
     3301 cacaatgata atcttcccca atggcattcc cttgataaaa aaatgactac agaaatcgtg
     3361 ataatgatag ttaattgatg cact
//
LOCUS       DBNHOH010000702         2479 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1388694, whole genome shotgun sequence.
ACCESSION   DBNHOH010000702 DBNHOH010000000
VERSION     DBNHOH010000702.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1388694"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            395..1390
                     /locus_tag="AC16V7_12235"
     CDS             395..1390
                     /locus_tag="AC16V7_12235"
                     /inference="COORDINATES: protein motif:HMM:NF024138.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR6257934.1"
                     /translation="MQPVEKIRSLDQIKTLSDPRRLAILRRLMDEPATLTQLGQALGE
                     HPAWIRYHLKQLEQAGLVEMISAQISGGYVEKYYQSTARAFILQQAILPEATREDVLV
                     LIGSDDIALGRLAQDLASRADLELLVLSVGSLDGLIALRQGLANLSSCHLLDSQSGEY
                     NLPYVRHLFPERSMKLVTLAHREQGLLLAPGNPRQVRGIPDLARADVTFINRNPGSGT
                     RLWLDLQLEKLGVNHELIRGYTREARTHTDIAQAIAQGTADAGLGLAAAARQLGLDFI
                     PLFQERFDLVISAEKIGEPSYRRLMEALNDRSFRSMAEGLGGYNTTHTGEELTTK"
     gene            1387..2244
                     /locus_tag="AC16V7_12240"
     CDS             1387..2244
                     /locus_tag="AC16V7_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6257935.1"
                     /translation="MRLHNRHLILLLIILSVMVSLSACATQPAAKEIILATTTSTQDS
                     GLLDVLIPIFEGRSGYTVKTVAVGTGQALKMGEDGNADVLLVHAPSAEKELMKGGFGV
                     ERLLVMHNDFVIVGPADDPAGIKGDVSAVEALVKIASAAAAFVSRGDDSGTHKAELAL
                     WKNGEIDPQGDWYIESGQGMAATLKIAGEKAAYTLTDRATYLATRQGLGLEILVEGDP
                     VLLNVYHVITVNPEKWPKVNNAGATAFAQFLIDGETQKVIGEFGVDKYGQPLFFPDAD
                     KTDADLGLE"
     gene            2245..>2479
                     /locus_tag="AC16V7_12245"
     CDS             2245..>2479
                     /locus_tag="AC16V7_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6257936.1"
                     /translation="MELIWQGILQAFGLLLRGDPEVFEITLLSLRVSGIATGISLLIG
                     LPLGTWLALGRFLGRDFLLSLINTGMALPPVVVG"
BASE COUNT          530 a          681 c          727 g          541 t
ORIGIN      
        1 gcccccacta aaacacccca ttttctctcc tcagtggtgg gcaatggctc caatgcgatt
       61 gccctacaag ctatttggcg aaaggtaaaa gacgcttgcg atgaaagctc gccacgatag
      121 ctttcaaatc ggtcatggcg aggagcgtag cgacgaagcc atctcctcac actgccacag
      181 agatcgcttc gcttgcagca cgctcgctcg agatgactaa gtgtgaaagg cgagtagcaa
      241 cagagtcagt tcatcggtca gtgaagcact ggaattactc agaggattta ttgacagact
      301 tttctcatcg tgttaaaatt aattttaacg ttaaattaaa atatttaact ttgtggcgtt
      361 gtttttttgc ctactccctt aaagaacctt ccctatgcag cctgttgaaa agattcgctc
      421 gctcgaccag atcaaaactc tctccgatcc gaggcgcctg gctatcctgc gccggttaat
      481 ggatgagccc gccacgctga cccaattggg gcaggcgttg ggtgagcatc cagcctggat
      541 ccgctatcac ctaaagcaat tggaacaggc tgggctggtt gaaatgatct ccgcgcagat
      601 cagtgggggc tatgttgaga agtattacca gtctacggcg cgggctttta tcttgcagca
      661 ggctatcttg cccgaagcga cccgtgagga cgtgcttgtc ctgatcggca gcgatgatat
      721 cgctttagga agactggcgc aggatctggc aagtagagct gatttggagt tgttggtcct
      781 gtccgtaggc agcctggacg gtttaatcgc cctgcggcaa gggctggcaa acctgagtag
      841 ctgccacctg ctggatagcc agagcgggga gtacaacctg ccttacgtgc gccacctgtt
      901 ccccgagcgg tcgatgaagc tggttaccct ggctcatcgc gaacagggtt tgctgctggc
      961 tcccggcaat ccacgccagg tacgcggcat ccctgacctc gcccgagccg atgtaacctt
     1021 catcaaccgc aatccaggct caggcacgcg cctgtggctg gatctgcaat tggaaaagtt
     1081 gggtgtaaat catgagctca tccgcggcta cacccgggag gcgcgcaccc acacggatat
     1141 cgcccaggct atagcccagg ggacagccga tgctggcctg ggattagctg ccgcagcgcg
     1201 gcagcttggc ctggatttta tccccctgtt ccaggagcgt ttcgacctgg tgatctcggc
     1261 cgaaaaaatt ggggagccaa gctaccgccg tttgatggaa gccctgaacg atcgctcctt
     1321 ccggagcatg gctgagggat tgggcggcta taacacaacc cacaccggag aggagctcac
     1381 cacaaaatga gactgcacaa tcgccatctt attctcctcc ttatcattct gagcgtcatg
     1441 gttagcctgt cagcgtgcgc tacccaaccc gctgcgaagg agatcatcct ggctactacc
     1501 accagcacgc aggactcagg cttgttggac gtgctcatcc cgatctttga agggcggagt
     1561 ggatacacgg tgaagactgt ggcggttggc actgggcagg cgctcaagat gggtgaagac
     1621 ggcaacgcgg atgtgctgct ggtgcacgct ccctccgctg agaaggagtt aatgaagggt
     1681 ggttttgggg tagagcggct gctggtgatg cacaacgatt tcgtcatcgt tggcccagca
     1741 gacgatccgg ccgggatcaa aggggacgta tcggcagttg aggctctggt gaagatcgcc
     1801 tcggcagcgg ctgcctttgt ctcgcgcggc gatgactccg gcacgcacaa agccgagctg
     1861 gcactttgga agaatggcga gatcgacccg cagggtgact ggtacatcga gtcggggcag
     1921 ggcatggccg ccacgctcaa gatcgccggt gaaaaggctg catacacctt aaccgaccgc
     1981 gccacttacc tggctacccg ccaggggctg gggctggaga tattggtgga gggcgacccg
     2041 gtcctcctga acgtctatca tgtcatcacc gtcaacccgg agaaatggcc caaggtgaac
     2101 aacgcaggcg ctacagcctt tgcccaattc cttatcgacg gcgagaccca gaaggtgatc
     2161 ggcgagttcg gcgtggataa gtacggccag ccgctgttct tccccgacgc tgataagacc
     2221 gatgcagatt taggattgga gtaaatggaa ttaatctggc aaggcattct gcaagcattt
     2281 gggctgctgt tgcgtggcga ccccgaagtc tttgagataa ccctactctc cctgcgtgtc
     2341 tcaggaatag caaccgggat cagcctgctc atcggcctgc ccctgggcac ttggctggcg
     2401 ctgggccgtt tcctggggcg ggatttcctg ctcagcttga tcaacactgg tatggctctt
     2461 ccgccggtgg tggtggggc
//
LOCUS       DBNHOH010000703         2377 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_748164, whole genome shotgun sequence.
ACCESSION   DBNHOH010000703 DBNHOH010000000
VERSION     DBNHOH010000703.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_748164"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1234
                     /locus_tag="AC16V7_12250"
     CDS             <1..1234
                     /locus_tag="AC16V7_12250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR6257937.1"
                     /translation="NDGEVFIVYSNIDRLERHMKELAPEDTEVIKEFTDGIRTMIHFP
                     MPVEKPQELYSPIDGFRMMYKMFPFLSFYRKFGKVTIEDYAKRFKNPFLRQAFLYIVN
                     LQNSPDFPMLGIMQVLAWMNQKTAGFPVGGSLEFAQSIGRRYIDLGGEIHYASRVVKI
                     LVKNNRAVGVRLADGSEHHSDIVISAADGHRAIFEMLDGEYINDKIKGYYEELPITPP
                     IIHIGLGVARSFEELPHSVTGTDLVLDEPITIGGQERSRMCVQIYNFDPSLAPAGKTV
                     VKVYYASDYEYWKKLIRDSARYEAEKELIADQVIAVLDQRFPGLASQVEMSDVATPMT
                     FEHYTGNWQGTFMGWQTSTKTLIMRMSKTLPGLKNFYMAGMWVEPGGGVPTAAMSGRN
                     VIQIICKLDKRPFKTKLP"
     gene            1409..2305
                     /locus_tag="AC16V7_12255"
     CDS             1409..2305
                     /locus_tag="AC16V7_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HIR6257938.1"
                     /translation="MKQLDKRQITGWIGVGFSIVITCTWAFWGIIETFHEGWYYESFL
                     SNVGLMLAQYLSPMLIFMGVTLISISWPRFGGGLHLILALLVAWFFQVFTNAAIFMLI
                     LPLIGIGALYWFGRPEPRKIALLLAAGLPMLILVISGVEPVLRVSQRFDDGNLQAQLV
                     YGNGVDLVWAPDGPGWPRTGVNWHEALQVCQHLSEDGLTLSFESLDIWRLPTVDEAVR
                     SMARHGQNSGGVWDAEIAEATYETTPDKESPLWNIHSQVIYWWTATTVDEEQAFIIVY
                     DGKVWPRSKQLSLAYLGFRCVK"
BASE COUNT          617 a          536 c          604 g          620 t
ORIGIN      
        1 aaacgatggt gaggtcttca tcgtctactc taatatagat cgcttggagc gacatatgaa
       61 agagcttgca ccggaggata cggaggtcat caaagagttt accgatggca tccgtacgat
      121 gattcacttc cccatgcccg tagaaaagcc acaagagctt tacagcccaa ttgacgggtt
      181 taggatgatg tataagatgt tccccttctt gagcttttat agaaaatttg gcaaggtaac
      241 aatcgaggac tatgccaaac gcttcaagaa tccattcctc cgtcaggctt ttttatatat
      301 tgtaaatctc caaaacagcc ctgacttccc aatgttaggg ataatgcaag tgcttgcttg
      361 gatgaaccag aagacagctg gatttccggt tggtggctcc ttggagtttg cccaatccat
      421 tggacgacgc tacattgatt taggaggcga gattcattac gcttcgcggg tagttaaaat
      481 cttggtaaag aataatcggg cagttggtgt gaggctggct gatggcagtg agcaccacag
      541 tgatatcgtc atttccgccg ctgatggtca ccgtgcaata tttgaaatgc tagatgggga
      601 atatatcaat gacaagatca agggctacta tgaggaactt cccatcaccc ctcccataat
      661 ccacattgga ctgggcgttg cgcgatcatt tgaagagctg ccgcacagcg tgacaggtac
      721 agacctcgtt ctcgatgagc caattaccat tggcgggcag gaacgcagtc gaatgtgtgt
      781 gcaaatttac aatttcgacc ccagcctggc gccggcaggc aagacggtag tgaaagttta
      841 ttacgcttcg gattatgagt actggaaaaa actaatccgg gattctgcgc gttatgaagc
      901 ggagaaagaa ctgatcgccg accaggtgat agcggtccta gaccaacgtt tcccggggtt
      961 ggcttcccag gtagagatga gtgatgtggc cacaccaatg accttcgagc actacactgg
     1021 taactggcag ggcaccttca tgggatggca aacttcaacc aagactttga tcatgcgcat
     1081 gagcaagacc ctgccgggac ttaagaactt ctatatggcg gggatgtggg tggagccggg
     1141 aggtggcgta cccacggcgg ctatgtcggg ccgcaatgta atccaaatta tatgcaagct
     1201 agataagagg ccgttcaaaa caaaacttcc atgaagttaa tatattgatg gcgcggtgac
     1261 ctgctcgggt aagttgagtc tggttatcga atatgtagaa aacaatatcg atacaggaaa
     1321 aatggaattt ctaaaatctc ttaggaaaaa atcatctggg agaaaaaact ctcaactgct
     1381 aaaacctact tttggacaca atgtcacaat gaagcaactt gataagcgcc aaattactgg
     1441 atggattggg gtgggttttt ccattgttat cacctgcacc tgggcatttt ggggcatcat
     1501 tgagaccttc catgagggtt ggtattacga gtcttttctc tccaacgtag gcttaatgct
     1561 cgcgcagtac ctgagcccaa tgctcatctt tatgggagtc acgctgattt caatctcttg
     1621 gcctcggttc ggcgggggtc tacacctgat cttagccttg ctggttgcct ggttcttcca
     1681 ggtatttacc aatgcagcca tctttatgct tatcctgcca ctgattggga ttggagcgct
     1741 ttactggttc ggtcgtcccg agccacgcaa gattgccctg ttattggctg ctggacttcc
     1801 catgctgata cttgtcatat ccggtgtaga gcccgtcctc cgtgtgtccc aacgctttga
     1861 tgatggcaat ctacaagccc aattggtata tggaaatgga gtcgatctcg tatgggcccc
     1921 tgatggacct ggatggccgc gtacaggggt aaactggcat gaagcactac aggtctgcca
     1981 acatctcagt gaagatggtc ttaccctttc cttcgaatca ctagacatat ggcgattgcc
     2041 gaccgtggac gaagccgtac gctctatggc tcggcacggt caaaacagcg gtggggtatg
     2101 ggatgcagaa attgccgagg ccacctatga aacaactccc gacaaggaat ctccattgtg
     2161 gaatatccat tcccaagtga tctactggtg gacggcgaca acggttgatg aagaacaggc
     2221 ctttatcatt gtctatgatg gaaaggtttg gccgagatca aagcaactca gtctggcata
     2281 tttagggttt cggtgtgtga aataacaatg cttacctgtg ttctgtaaca gttacgcata
     2341 aatttgtaat tccccagtga aataccctct tgtttaa
//
LOCUS       DBNHOH010000704         3395 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_427454, whole genome shotgun sequence.
ACCESSION   DBNHOH010000704 DBNHOH010000000
VERSION     DBNHOH010000704.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_427454"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1226
                     /locus_tag="AC16V7_12260"
     CDS             <1..1226
                     /locus_tag="AC16V7_12260"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HIR6257939.1"
                     /translation="TRGDIPDYQAAALAMAILLRGMTPQETADLTMAMANSGEILDLT
                     GVVPIAVDKHSTGGVGDKTTLVVEPLVAACGLAVGKMSGRGLSFSGGTLDKMESIPGY
                     RTDLSTEEFIQQLKSVGLVLTGQTADLAPADGKLYALRDVTATVQSLPLIASSIMSKK
                     IAAGAQAIVLDVKVGLGAFVQNLDEGRQLAEAMVSIARFIGRRAVALLSDMNQPLGNA
                     VGNALELKEAIQTLHGSGPADFLEHCLEVAGYMLAIGGKAADQNEGMQLAHAALDDGR
                     GWEFFKRLVAAQGGDVAVIENPDRLPKASFVKEVASPRSGYLSAIHARIVGETVVSLG
                     GGRAKKGDPIDHAVGAIIHHKVGGHVEAGEPLFTIHANDQARLEDAHQRLLQAHSWSD
                     GPVEPLPLFYGVVQE"
     gene            complement(1284..2375)
                     /locus_tag="AC16V7_12265"
     CDS             complement(1284..2375)
                     /locus_tag="AC16V7_12265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020490126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR6257940.1"
                     /translation="MNILIFGAGAIGTYIGGSLILRGDRITFIEQPQAVEEITRRGLY
                     LDLSAARRKPAPVDTQPSITDLHPNIVLPPSYFYCVRSLEEALKLGPYDLAIFALKSF
                     DTQPVIESLQPFLSDLPTFLCLQNGVENEVALANALGPDRVIAGTVTSAVSRRNIGEI
                     ILERLRGIGVALGHPISRRLVYILNEAGLNAQVYTSSAEMKWSKLLTNLLANATSAIL
                     DMTPAEVFAHPGLYRLEIDQLVEALRVMDAQNLRVVNLPGTPVRLLALGTRLPIFLSK
                     PLMQRSVGKGRGAKMPSFHIDLHSGRGKSEVDFLNGAVARFGKRLGVPTPVNHTLNET
                     LLALTEGKTPLEFYARKPQVLLDSMGERE"
     gene            complement(2372..3286)
                     /gene="fmt"
                     /locus_tag="AC16V7_12270"
     CDS             complement(2372..3286)
                     /gene="fmt"
                     /locus_tag="AC16V7_12270"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019023220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR6257941.1"
                     /translation="MKPHLVFMGSPKFALPTLSALAENYSIQGVVTQPDRPAGRGKQL
                     TPPVIKSFAVDLGLPVIQPERLKDPAAMQQLANWAPDLIVVVAYGQILRQTVLDLPRN
                     GCINVHASLLPRWRGAAPIQAAILAGDEWTGVTIMRMDSGVDTGPILSQRGIPILCED
                     TAGSLSERLAELGAELLIETLPAYLMGELHPQPQSESGVTYAPMLKKGDGQLDFTQSA
                     LDLARRVRAFNPWPGATTQWKGQPLRIHSAHAIEDYLSPPGQRLVYGPKPAIGTGQGT
                     LVLDEVQPAGKKAMPGDVFLRGARDWEE"
BASE COUNT          776 a          849 c         1007 g          763 t
ORIGIN      
        1 acacgcgcgg cgatatcccc gattatcagg cggctgctct ggctatggca attcttctca
       61 ggggcatgac cccacaggaa actgccgacc tgacaatggc gatggccaat tctggcgaga
      121 tactcgacct gaccggggtt gtgcctatcg ctgtggataa acactctacg gggggagtgg
      181 gtgataagac taccttggtg gtggagcccc tggtagcagc ctgcggtctg gcggtaggca
      241 aaatgtcggg tcgcggcctg tcttttagcg gcggtacact cgataaaatg gagtctatac
      301 cgggttaccg cacggacctg agcactgagg agtttatcca gcagttgaag agcgttggtt
      361 tggtgctcac cggtcaaacc gcagacctgg ctcctgcgga tggtaagctc tacgcgctgc
      421 gtgacgtgac cgctaccgtt cagtcattgc cactcattgc gtcatccata atgagcaaga
      481 aaatcgccgc gggagcgcag gctattgtat tggatgttaa ggtggggctg ggagctttcg
      541 tgcaaaacct ggacgaaggg cggcaattgg ccgaggcgat ggtatcaatc gcccgcttta
      601 ttgggcggag ggcggtggcg ctgctctcgg acatgaacca gcctttggga aatgccgtgg
      661 ggaacgcatt ggaattgaaa gaagccatcc agaccttgca tggtagcgga ccagccgact
      721 tccttgagca ctgcctggag gttgctggat acatgctggc aattggagga aaagcagcgg
      781 atcagaatga agggatgcag ctagcccatg ctgcgctgga tgatggccga ggttgggaat
      841 tcttcaaaag gttagtggct gcgcaaggtg gcgatgttgc tgtaatagaa aatccagacc
      901 ggttgccaaa ggctagcttt gtaaaagaag tggcttcgcc gcgttccggc tatctcagtg
      961 cgattcacgc ccggattgtg ggggagaccg tcgtatcgtt gggtggcgga agagcaaaaa
     1021 agggtgaccc gattgaccat gccgtaggtg cgatcattca tcataaagtt ggaggccatg
     1081 tggaagcagg ggaaccgctt ttcaccattc acgccaatga tcaagcacgc cttgaggacg
     1141 ctcatcagcg gttgctgcag gcgcattctt ggagtgatgg tccggtcgaa ccgttacctt
     1201 tattttatgg tgtagttcag gaataaaggc gcgacgctaa aatatgggga aaagctctat
     1261 ggtaggggtg ttaggtgcct tttctattcc ctttccccca tgctatccaa taacacctgc
     1321 ggtttgcgcg cgtaaaactc caatggtgtc ttcccttcgg ttagcgccag cagggtttca
     1381 ttcaaggtat ggtttacggg cgtcggcacg cctagccgct tgccaaaacg ggcaacagcg
     1441 ccgttgagaa agtccacctc gcttttaccg cgcccgctgt gtagatcgat gtgaaaagac
     1501 ggcatctttg caccgcgccc cttgccaaca ctgcgttgca tcaaaggctt ggagagaaaa
     1561 atcggtagtc gggtacctaa agccagcaac cgtacaggcg ttccaggcaa gtttacaaca
     1621 cgcaaattct gggcatccat tacccgcagc gcctcaacta actgatctat ctccagccgg
     1681 tataaacccg ggtgtgcgaa aacttcagcg ggggtcatat ccaagatggc cgaggtggca
     1741 tttgccagaa ggttggtgag cagtttggac cacttcatct ctgcagagga tgtgtaaacc
     1801 tgtgcattca atccagcctc gttcaagata tagaccaacc gtctggagat tgggtgcccc
     1861 agcgcgaccc caatgccgcg cagccgctcc aagataatct cgccgatatt gcggcgactt
     1921 acggcgcttg tcactgtccc ggcgatcacg cgatcaggcc ccaaggcatt tgccagggcg
     1981 acttcattct ctaccccgtt ttgcaagcaa agaaaggtgg gcaggtcgct caggaagggt
     2041 tgtaaacttt ctatcacagg ctgggtgtcg aaagatttaa gggcaaatat agcaagatcg
     2101 tatggtccta gtttaagtgc ctcttccaag gatcgcacac agtagaagta ggaaggtgga
     2161 aggacaatgt tggggtggag atcggtaatg gatggttggg tatcgacggg cgcaggcttt
     2221 cgccgggcag ccgacagatc aaggtacagg ccgcgacgcg tgatctcctc gacggcttgg
     2281 ggctgctcga taaaggtgat acggtcgccg cgtaaaatca agctaccgcc gatatacgta
     2341 cctatagcgc cagcgccaaa gattaagatg ttcattcttc ccaatctctt gcgccgcgca
     2401 ggaaaacgtc gcctggcata gcctttttgc cagctggctg cacttcatcc agcacaagcg
     2461 tgccttgtcc tgtaccgatg gcgggttttg gcccatagac gagacgttga ccaggcgggg
     2521 aaaggtaatc ttcaatcgcg tgagcgctat gaatccgaag tggctgtcct ttccactggg
     2581 tggtagctcc tggccagggg ttaaatgccc ttactcttcg ggccagatca agcgcagatt
     2641 gcgtgaaatc caattgtcca tccccttttt tcaacatggg agcgtaggtg acccccgatt
     2701 cggattgggg ctgtgggtgc aactccccca taagatatgc tggcaaggtt tctattagta
     2761 gctctgcccc cagttccgcc agtcgctcgg agagagaacc ggcggtatct tcgcacaaaa
     2821 tggggatacc gcgctggctg agaatagggc cggtgtccac accgctgtcc atgcgcatga
     2881 ttgtgacgcc agtccactcg tcgccggcta ggatcgcagc ctggatgggg gcagccccgc
     2941 gccagcgcgg cagcagggaa gcatgcacat tgatgcagcc attcctcggc agatcaagca
     3001 ctgtctggcg caggatttgt ccataggcta caacaacaat gaggtcagga gcccagttag
     3061 ccaattgctg catggctgcg ggatctttca gtctctcagg ctggataacg ggtagcccca
     3121 agtctacagc aaaggatttg atcacaggcg gtgtgagctg cttgccgcga ccggcaggcc
     3181 ggtctggttg ggtgaccacg ccctggatgg aataattctc agctagcgcg cttaacgtgg
     3241 gaagggcgaa ttttggcgag cccatgaaaa ccaggtgagg tttcatgggc tctattctag
     3301 caggttgtca tggtcaaaac aaggttgagt gtagaaaagt ttgaggttaa catgacaatt
     3361 taataactga gtcaattgta aggaaatatg aggga
//
LOCUS       DBNHOH010000705         1711 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_876461, whole genome shotgun sequence.
ACCESSION   DBNHOH010000705 DBNHOH010000000
VERSION     DBNHOH010000705.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_876461"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..421)
                     /locus_tag="AC16V7_12275"
     CDS             complement(68..421)
                     /locus_tag="AC16V7_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR6257942.1"
                     /translation="MTKANVSMNVRTASPNTSIVDIVGEINAFAENVLMDAYTQASSA
                     GAHNILLNFSGLEYMNSSGIGLLVTLLIRVNRQKQRLSAFGLSEHYQQIFELTRLNEA
                     IGIYPDEAAALASLG"
     gene            complement(462..806)
                     /locus_tag="AC16V7_12280"
     CDS             complement(462..806)
                     /locus_tag="AC16V7_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR6257943.1"
                     /translation="MAQHLFEARLRQVSGKAIIDLQGEINAFADANLSEAFTQAEDTD
                     CPDILLNFSEVSYINSTGIALIVGLLARARKNNRRLLVCGLSDHFTEIFEITRLTDYM
                     AIYPNELEALAV"
     gene            complement(787..>1711)
                     /locus_tag="AC16V7_12285"
     CDS             complement(787..>1711)
                     /locus_tag="AC16V7_12285"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding SpoIIE family protein phosphatase"
                     /protein_id="HIR6257944.1"
                     /translation="VTCLCIVLDPISGYLRFANAGHNLPSLCKTDDVYELRATGMPLG
                     LMPGMTYEEKDVTLEPGDSLLIYSDGLVEAHNPQQEMFGFPRLRECLPCSPQGGWQGE
                     GTLIEYLLEKMAEFTGPTWEQEDDVTFVTLERQPSQSASHSAEDQADVKGEAWQILAE
                     FSLPSEPGNEITAMNLVMEVVRDLISPPVRLERLKTAVAEATMNAMEHGNRYQADLPV
                     ELRVLLSPTRLAVRITDHGGANPIPETEAPDLEAKLAGLQSPRGWGLFLIKNMVDEMN
                     IHVSDAGHGSIHHTVELIISLKGDDHGSAPV"
BASE COUNT          405 a          479 c          427 g          400 t
ORIGIN      
        1 tgatacctct atttgtaata cacatccaga atggcgattc tgtaactaat aaaataggat
       61 cgagttctta ccctaaggac gccaatgcag cagcctcatc cgggtaaatg ccgatcgcct
      121 cgttcagacg ggttaactcg aagatttgct ggtaatgctc gctcaacccg aaggccgaca
      181 agcgctgttt ctgccggttg acgcggatga gcagcgtgac gagcaatcca attccagagc
      241 tgttcatata ctccagcccg ctgaaattca gcaaaatatt gtgcgcccca gcactgctgg
      301 cctgagtgta agcgtccatt aagacgttct cggcaaaggc gttgatctct ccaacgatat
      361 ccacaatgct ggtattcggg ctggctgtgc gcacgttcat ggaaacatta gctttggtca
      421 tctttaaatt tctccttagc aatactctag tttttttcct ctcagactgc cagggcttcc
      481 agctcgtttg gatagatcgc catgtaatca gtcaaacggg taatctcaaa gatttcggta
      541 aagtgatcgc tcaatccgca gacgagcaag cggcggttat ttttacgcgc ccgggccagc
      601 aaaccgacga tcagggcaat gccagtgcta ttaatatagc tcacctcact aaagttgagc
      661 aagatatccg ggcaatctgt gtcctctgcc tgcgtaaacg cctcacttaa attggcgtca
      721 gcgaaggcat tgatctcccc ttgcaggtca attatggctt tgccagacac ttggcgcaac
      781 ctggcttcaa acaggtgctg agccatggtc atctcctttc aagctaatta ttagctcaac
      841 agtatggtgg atgctgccat gtccagcatc gctgacatgg atgttcattt catccaccat
      901 atttttgatc aagaaaagcc cccagccacg cggcgattga agaccggcca atttagcctc
      961 caggtcgggc gcttcagtct cgggaatagg attggcaccc ccatgatcag tgatacgcac
     1021 cgcaaggcgg gtgggggaga gcaaaacccg cagctccacc ggcaaatccg cctggtagcg
     1081 gttgccatgt tccatagcgt tcatggtagc ttcggcgaca gcggttttca ggcgttccaa
     1141 acgaacgggg ggcgagatca aatcccgcac tacctccata accaggttca tggccgtgat
     1201 ctcgttcccc ggctcactgg gtagagagaa ttcggccagg atctgccagg cttcgccttt
     1261 gacatccgcc tgatcttccg cagaatggga agcggattgg gagggctggc gctccagggt
     1321 gacgaaagtt acatcatctt cctgttccca ggtcgggccg gtgaattcag ccatcttctc
     1381 cagcaagtac tcaatcaacg tgccctcacc ttgccagcca ccctgcggcg agcacggcaa
     1441 gcactcacgc aagcgcggaa agccaaacat ctcctgctgc gggttgtgcg cctccaccaa
     1501 tccatcactg tagatcagca ggctatcgcc gggttccagt gtcacatcct tctcttcata
     1561 ggtcatgcct ggcatcagac ctagcggcat gcccgtagct cgcagctcgt acacatcatc
     1621 ggtcttacat agggaaggga gattatgccc ggcgttggca aagcgcagat acccactaat
     1681 gggatccagg acgatgcata agcaggtgac a
//
LOCUS       DBNHOH010000706         5916 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_170203, whole genome shotgun sequence.
ACCESSION   DBNHOH010000706 DBNHOH010000000
VERSION     DBNHOH010000706.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5916)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5916
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_170203"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            304..2211
                     /gene="acs"
                     /locus_tag="AC16V7_12290"
     CDS             304..2211
                     /gene="acs"
                     /locus_tag="AC16V7_12290"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetyl-CoA synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HIR6257945.1"
                     /translation="MAQSAKKDTQLEGEVYYPSPEITQAAHIQEYESLYQYSIQDPEG
                     FWGERADELDWYKKWDKVLDDSKAPFFQWFVGGKTNIVHNALDRHLKTYRKNKLALVW
                     EGEPGDMRTFSYFSLHREVCKFANVLRSMGVKKGDIVTIYMPRIPEMVIAMLACAKIG
                     APHSVVYGGFSVEALAERIEDAQSRVLITADGGWLRGKIVALKDIADEAIARQPTIEN
                     CIVVKRTGQDIYMEAGRDYWYHDLMGLPIASITSYTEELDAEDPLFILYTSGTTGRPK
                     GVVHTHGGYMVYTYSTLKYVFDIKDEDRWWCAADPGWVTGHSYIVYGPLINGATSFMY
                     EGAPNHPYPNRWWKMVENHGITILYTAPTAIRGLMRFGEAWPNRHDLSSLRLLGSVGE
                     PINPEAWKWYHTVIGKGRCPIMDTWWQTETGGFMISPLPITPLKPGSATRPLFGIDAE
                     VVDDDGKPVPAGEEGYLVIKKPWPGMLRTILKDPERYKEQYWGKYGNMYQAGDSARKD
                     KDGYIWVIGRIDDVIKVSGYRLGTAEVESALVSHPDVAEAAAIGLPHELKGNAIHTYV
                     ILKAGVEKSDKLAEELRAHVGHEIGPIARPESVTFMDSLPKTRSGKIMRRLLKARALG
                     LPEGDISTLEE"
     gene            2215..3186
                     /locus_tag="AC16V7_12295"
     CDS             2215..3186
                     /locus_tag="AC16V7_12295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6257946.1"
                     /translation="MKNRKFGRTGWMVSDIGYGMWGMGEWTGSDDQESLQSLQRAVDL
                     GCNFFDTAWAYGAGHSEGLLGDLVRANPDRKLYTATKVPPKNRKWPSRREFALDDCYP
                     PDHIRFYVHSSLVNSGLDRFDLVQFHTWEDSWVDDSRWAAAFDDLKREGVVGAVGISI
                     NRWEPWNGVRAVKSGLIDAVQVIYNIFDQNPEDELFPACREMDVGIIARVPFDEGTLT
                     GTLTLDSKWPSGDWRNSYFVPENLKSSVEHAERLRPLVPPGMTMPEMALRFILNNPDV
                     STIIPGMRKLRHVESNIACSDAGPLPEALHQELRDHRWDRTPSWWSQ"
     gene            complement(3295..4038)
                     /locus_tag="AC16V7_12300"
     CDS             complement(3295..4038)
                     /locus_tag="AC16V7_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR6257947.1"
                     /translation="MTTTIVSSIALFIFAAAILYASVGHAGASGYLAVMALFSVAPEI
                     MRPTALALNVLVATIGTIKFYRAGYFSWSVFWPFALTSIPFSFIGGSLSLPGSVYKVI
                     VGLVLLYAAFRLFWTKKDDLNPLAVKKGPLPVALVFGAGIGLLSGLVGVGGGIFLSPL
                     LILMKWADVKQTSGVSAAFILVNSIAGLSGQIASLTHLPGAIPYWAVAAGIGGWIGAE
                     YGSRRLGSLTLRRLLALVLVVAGVKMILI"
     gene            4508..>5916
                     /locus_tag="AC16V7_12305"
     CDS             4508..>5916
                     /locus_tag="AC16V7_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR6257948.1"
                     /translation="MPFKEVPNKVDFPAQERELLDFWKKSNAFQKLRELHSNDPHWSF
                     IDGPITANNPMGVHHGWGRTYKDLFQRFWTMRGRKLRYQNGFDCQGLWVEVEVEREMG
                     FRTKKDIEEFGLEQFVRKCKARVLRYAAVQTEQSIRLGYWVDWNDPDQLRWLADLMEE
                     DPYQVITVQGTHGPVTNTVEQIVGQLGLADLGGSYFTFSNENNYMIWTFIKKCWEKGW
                     LYRGADVMPWCPRCATGISQHEIVTDGYAELTHDSITLRLPLRATDGSRRTSGGLPES
                     LLVWTTTPWTLTSNVAAAVGPDLTYAKVRQGDEIFYLSKGTLHMLRGPYEVLGELQGV
                     DMQGWTYDGPFDDLPPAQITGGYTHLTELTQGVTISPAQAHRVILWEAVGETEGTGIV
                     HIAPGCGAEDFQLGREYGLPLVAPLDDEGYFVDNFAWLSGLHVADVSQPIFEDLRRKD
                     LLYTVEPYTHRYPTCWRCRS"
BASE COUNT         1330 a         1714 c         1690 g         1182 t
ORIGIN      
        1 ctgcgcccgc ccaaactccc cctcttccct attcaactgc gtaagtccca aaataagaaa
       61 tatcattatt caacgatgtt tttaggtaca actaaatatt attttagttg atagtgttgt
      121 tcttgtcaag tgtgattgtt tatggctttt cctgtcaaca ttgacaacat gccttccttt
      181 tcttaaaatg aaggcctata caggttctca ttctctatta ctctattgct ctcaatctac
      241 tctgtctagc ctgtaatgtg ctatctttaa ccaaatacag caacccacca aaggagagga
      301 aacatggctc agtccgctaa aaaagatacc cagttagaag gcgaggtgta ttacccctca
      361 ccagaaatca cccaggcagc tcatattcaa gaatatgagt cgctctacca gtactccatc
      421 caagaccctg aaggtttctg gggtgagcgc gccgatgagt tagattggta taaaaaatgg
      481 gacaaagtgc tggacgacag caaggcgcct ttcttccagt ggttcgtggg cggcaagacc
      541 aacatcgtgc ataacgccct ggaccgccac ttgaagacct accgcaagaa caagctggct
      601 ctggtgtggg aaggcgagcc gggtgatatg cgcactttct cgtacttttc cttgcaccgc
      661 gaagtgtgca aatttgccaa tgtgctgcgc agcatgggtg tgaagaaagg cgacatcgtc
      721 accatctaca tgccgcgcat ccccgagatg gtcatcgcca tgctggcctg cgccaagatt
      781 ggcgcccccc acagcgtggt gtacggcggc ttcagcgtgg aggcgctagc agagcgaatc
      841 gaggacgccc agagccgggt gctgatcacc gccgacggcg gctggctgcg cggcaagatc
      901 gtcgctttaa aggatattgc tgatgaggcc atcgcccgcc agcccaccat cgagaactgc
      961 atcgtggtca aacgcaccgg gcaggatatc tacatggaag ctgggcgtga ttactggtat
     1021 cacgacctga tgggcctgcc cattgcctcc atcacgtctt ataccgagga gttggatgcc
     1081 gaagacccgc tgtttatcct gtacacttcc ggcactaccg ggcgccccaa gggggtggtg
     1141 cacacgcacg gcggctacat ggtctatacc tactccacgc tgaagtatgt cttcgatatc
     1201 aaggacgagg accgctggtg gtgcgctgca gaccccggct gggtgaccgg gcactcatac
     1261 atcgtctatg gtcccctgat caatggcgcc accagcttca tgtacgaggg tgcccccaac
     1321 catccttacc ccaaccgctg gtggaagatg gtggagaacc acggcatcac catcctgtat
     1381 accgccccca ccgccatccg cggcctgatg cgctttggcg aggcctggcc taatcgtcac
     1441 gacctgagca gcctgcgcct gctgggctcc gtaggcgagc cgatcaaccc tgaggcttgg
     1501 aaatggtatc acaccgtcat cggcaaggga cgctgcccca ttatggatac ctggtggcag
     1561 accgagacag gcggctttat gatctcgcca ctgcccatca ctccgctcaa gcccggctct
     1621 gccacccgcc cgctctttgg aatcgatgcc gaggtggtgg atgacgacgg caagcctgtg
     1681 cccgcgggag aagagggcta cctggtgatc aagaaaccct ggcccggtat gctgcgcacc
     1741 atcctgaaag accccgagcg ctataaggag cagtactggg ggaaatatgg gaacatgtat
     1801 caggccggcg attccgcccg caaagacaag gatggctata tctgggtcat cgggcgtatc
     1861 gacgatgtca tcaaggtcag tggctaccgg ctgggcaccg ccgaggtgga gagcgctctg
     1921 gtcagccacc cagatgtggc ggaggccgct gcgattggtc tacctcacga gctaaagggc
     1981 aacgccattc acacctacgt gatcctcaag gcgggggtag agaagagcga caagctggcg
     2041 gaggagctgc gcgcccacgt cggtcacgag atcggcccca tcgcccgccc cgagagcgtc
     2101 acttttatgg atagcctgcc caaaactcgc tccggtaaga tcatgcgccg ccttctcaaa
     2161 gcacgtgcct tgggcttgcc cgagggagat atcagcacat tggaagagtg atacatgaaa
     2221 aaccgcaagt ttggcagaac gggatggatg gtcagcgaca tcggctatgg catgtggggc
     2281 atgggcgagt ggacaggctc cgacgaccag gaatcgctcc agtccttgca gcgcgccgtc
     2341 gatttgggct gcaacttttt cgacaccgcc tgggcatacg gggctgggca cagcgaaggg
     2401 ctgttggggg acctggtgcg ggcaaacccg gatcgcaagc tgtacaccgc cactaaagtt
     2461 ccgcccaaga accgcaagtg gcccagccgc cgtgaatttg ccttggatga ttgctaccca
     2521 cccgatcaca tccgcttcta cgtgcactca agcctggtaa actccggatt ggatagattc
     2581 gatctggtgc agtttcatac ctgggaagat agctgggtgg acgactcgcg ctgggcggca
     2641 gccttcgacg acctcaagcg cgagggggta gtgggtgcgg taggcatcag catcaaccgc
     2701 tgggagccgt ggaatggtgt cagggcggtg aaatctggct tgatcgatgc agtgcaggtg
     2761 atctacaaca tttttgacca gaatcccgag gatgagctct tccccgcctg ccgggagatg
     2821 gacgtaggga tcattgcccg cgtccccttc gacgagggca cgctgaccgg caccctgacg
     2881 ctggacagca agtggccctc aggcgactgg cgcaattcct acttcgtgcc ggagaatttg
     2941 aaaagcagcg tggagcatgc cgagcggctc agaccgctcg tgccgcccgg catgaccatg
     3001 cccgagatgg cgctgcgctt catactcaac aaccccgatg tcagcaccat catccctggc
     3061 atgcgtaaat tacgccatgt ggagagtaac atcgcctgca gcgacgccgg tccattgccc
     3121 gaggcgctgc accaggagct gcgagaccac cgctgggatc gcaccccctc ctggtggtcg
     3181 caataagagc gggtctttat taggacttac gcagttgaag aggaaaggag gggggtttgg
     3241 gcgggcgatg cctgcccaaa cccccctgcc acgtatccaa ctgcgtaact tcttttatat
     3301 taagatcatc ttcaccccgg caaccaccag gaccagagcc aacaggcggc gcaaggtaag
     3361 gctacctaag cggcgactgc catattccgc cccgatccaa ccgccgatgc ccgccgccac
     3421 cgcccagtag ggtatagcac caggcagatg ggtcaggctg gcgatctgtc ccgacagccc
     3481 ggcgatcgaa ttcaccagaa taaacgccgc cgagacaccc gaggtctgct tgacatccgc
     3541 ccatttcatg aggatcagca gtgggctgag gaaaatacct ccgcccacgc ccaccaaacc
     3601 ggacagcagc ccaatcccag ccccgaaaac cagcgccaca gggagcggac ccttcttaac
     3661 cgccaggggg ttcagatcat cctttttcgt ccagaaaagc cgaaaagcag cgtagagcag
     3721 caccaggcct acaatgacct tgtacactga gccgggcagc gacaggctgc ctccgataaa
     3781 tgaaaaggga atggatgtca gggcaaacgg ccaaaaaacc gaccaggaaa aatatccagc
     3841 gcggtagaac ttgattgtac caatcgtagc taccaggacg tttagggcca gtgcagtggg
     3901 tctcatgatc tcgggcgcca cgctgaaaag cgccatgaca gctaggtatc cggaggcgcc
     3961 ggcgtgcccc acagaggcgt agaggatggc agccgcgaag atgaatagcg caattgaaga
     4021 tacgattgtg gtggtcaatt tagcccttaa ataaaatggc attatatcac cgccgttgac
     4081 catctcgcca aattatggca taatccaacc caacttaacc gaaaaaggcg atgatggaaa
     4141 cgagtaagcc tgaccaggct ggcagcgaac ccgggagtgg tgtgagccgg ggcagaccat
     4201 cgggctgaaa atccttccgg agccgcaagt cgaacgtccc caaagtagat gcgccgggcc
     4261 tgccccccgt tatccgggcc agtgattaga gaattagcga tcagacaact gatcactggt
     4321 atctgttcac tgagctgagt tgcccgaagt ctcgggaacc agggtggtac cgcgggagcg
     4381 atgtttgtga ctctcgtccc tgagaggggc gggagttttt ctttttgtga gcgcgaaggg
     4441 cagggtgatt gttatttttt ttcgctgccc atccgctctc gaatttctaa tttcgtttgg
     4501 agttgctatg ccatttaagg aagtgcccaa caaagtagat ttccctgccc aggagcgtga
     4561 gctgctcgat ttctggaaaa agagcaacgc cttccagaag ctgcgcgagc tgcattcgaa
     4621 cgaccctcac tggtcattta tcgacggtcc aattaccgcc aacaacccca tgggcgttca
     4681 ccatggttgg ggacgcacct ataaagacct gttccagcgc ttctggacca tgcgcggccg
     4741 caagctgcgc taccagaatg gcttcgattg ccagggtttg tgggtagaag tcgaagtcga
     4801 gcgcgagatg gggtttcgca ccaagaaaga catcgaggag tttggcctag agcagttcgt
     4861 gcgcaagtgc aaggcgcgcg tgctgcgcta tgccgctgtg cagacagagc agtccatccg
     4921 cctcggttac tgggtggact ggaatgaccc cgaccaactg cgctggctcg ccgacctcat
     4981 ggaagaagac ccctaccagg tgatcaccgt tcagggtaca cacggcccag taaccaacac
     5041 ggtggagcag atcgtcggtc agctcggttt ggcagacctg ggtgggagct acttcacgtt
     5101 ctcaaatgag aacaactaca tgatctggac cttcattaag aagtgttggg agaagggctg
     5161 gctgtatcgc ggcgccgacg taatgccctg gtgcccgcgc tgcgccaccg gcatcagcca
     5221 gcatgagatc gtcaccgatg gctacgccga gctgacccat gactccatca ccctgaggct
     5281 cccattgagg gcaacggacg gtagtcggcg cacttcgggt ggcctgcccg aatctttgct
     5341 ggtctggact accacccctt ggacgctcac cagcaacgtc gcagctgccg ttggccctga
     5401 cctgacctat gccaaggtgc gccaggggga cgagatcttt tatctctcta agggaacact
     5461 gcacatgctg cgtggtccgt acgaggtgct gggggagctg cagggcgtcg atatgcaggg
     5521 ctggacctat gacggcccct ttgacgacct gccccccgcc caaataactg gcggctatac
     5581 ccatctcacc gagctcaccc agggtgtgac aatttcgcct gctcaggcgc atcgggtcat
     5641 cctatgggaa gcggttggcg agacggaggg tactggtatc gttcacatcg ccccgggctg
     5701 cggtgccgag gacttccagc tagggcgaga atatggcctg ccactggttg ccccgcttga
     5761 cgatgaaggc tacttcgtag acaatttcgc ctggctgagc gggctgcatg tagcggatgt
     5821 atcgcagcct atcttcgaag atttgcgccg caaagatctg ctatacactg ttgagcccta
     5881 tacccaccgc taccccacct gctggcgctg ccgttc
//
LOCUS       DBNHOH010000707         2167 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1389045, whole genome shotgun sequence.
ACCESSION   DBNHOH010000707 DBNHOH010000000
VERSION     DBNHOH010000707.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1389045"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /gene="meaB"
                     /locus_tag="AC16V7_12310"
     CDS             <1..816
                     /gene="meaB"
                     /locus_tag="AC16V7_12310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HIR6257949.1"
                     /translation="RYPPGGDSPQRVGVVAVDPSSPFTGGAILGDRVRMRDLAGDPGV
                     FIRSMASRGTLGGLATATASMVLALDAAGFEVILIETVGAGQAEVDIARLAHTTLVVE
                     APGLGDDIQAIKAGILEIADILVVNKADRPGVENTERALRGMLQLGHPAPRVYLHHGQ
                     TERLTPPADLEADNLLWQTPVVRTVATEGTGVDGLYQEIARHRRYLHESGEWERRERA
                     RLQSELDILLQAALVSRWRETVSQEAYRGTVDRLVRRDISPWQAVESLLDGHG"
     gene            845..1381
                     /locus_tag="AC16V7_12315"
     CDS             845..1381
                     /locus_tag="AC16V7_12315"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6257950.1"
                     /translation="MIYGERIRLRAPERDDLPRFVGWLNDPEVRQNLFMFLPLSMAEE
                     EHWFEDVLKQPAAERPMVIEILDGESWKPVGNCGFIHIDWRCRAGEVGIFIGDKSRWN
                     QGYGTEAMRLLLRHGFNTLNLNRIFLRVFDTNARAMRTYEKAGFVHEGRLRQAEYQDG
                     KYIDMLVMSVLYSEWKEN"
     gene            1396..>2167
                     /locus_tag="AC16V7_12320"
     CDS             1396..>2167
                     /locus_tag="AC16V7_12320"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIR6257951.1"
                     /translation="MLTHYLHRDTNMYGDIKLFAGTACPELAGTIAKYLELPLSGRDV
                     TLFPNDNLFIRLHKSVRGQDVYVIQTTSRPVHRNLMELLIMLQTLRLDSAGRVTAVVP
                     YMCYARSDKKDKPRVPITARLVADMIEIAGADRYMTLDLHAGQIQGFFSIPGDVLTAF
                     HILTAHVEKMLPALKDPVVVAADLGFAKKGRNFAATIQAPIAFIEKRRSAKEDQPIAL
                     TIIGDVKDRDVIIIDDEVDTARSVEQAVNLVKAHGARTV"
BASE COUNT          457 a          585 c          655 g          470 t
ORIGIN      
        1 cgctacccgc ctggtgggga ttccccgcag cgcgttgggg tggtggctgt cgacccctcc
       61 agcccgttta caggcggggc gatcctgggt gaccgggtac gcatgcgcga tctggcgggc
      121 gatccgggtg tgtttatccg ctcgatggcc tcgcggggca cgctgggtgg cttggcaaca
      181 gccaccgcca gcatggtact ggctcttgac gcggcgggct tcgaagtaat cctaattgag
      241 acggtcggcg cgggtcaggc agaggtggat attgcccgcc tggcgcacac cacactagtt
      301 gtcgaagccc ctggtctggg tgatgatatt caggctatta aggctggcat ccttgagatc
      361 gctgatatcc tggtggtgaa caaagccgac cgacccggcg tagagaacac cgagcgtgcc
      421 ctgcgaggta tgctgcaatt ggggcatcct gctccgcgtg tatacttgca tcacggtcag
      481 acggaaaggt taactccgcc tgcagacctg gaagctgaca acttattgtg gcagactccc
      541 gttgtgcgca ccgtcgccac cgagggcacc ggcgtggatg gtttatatca ggagattgcc
      601 cgccaccggc gctacctgca tgagagcggc gagtgggagc gccgtgagcg cgcccgcctg
      661 caatctgaac tggatatact gctccaggcg gctctggtct cgcgctggag agagacggtc
      721 tcccaagagg cgtatcgagg caccgtggac aggttggtgc gcagagacat ttccccctgg
      781 caggcggtgg agagcttgct cgacggtcac ggataataac cagcatccta ctgaggagag
      841 agcaatgatc tacggcgaac gcatccgact gcgcgctcca gagcgagatg acttgcctcg
      901 ctttgttggt tggctgaacg acccggaggt acgccagaac cttttcatgt tcttgccact
      961 ctcgatggca gaggaagagc actggttcga ggatgtgttg aagcagccag cagcagagcg
     1021 cccgatggtg atcgaaatcc tggacgggga gagctggaag ccggtaggta actgtggctt
     1081 tatccacatc gattggcgct gccgggccgg cgaggtgggc atcttcatcg gagataaatc
     1141 acgatggaac cagggctatg gcacagaagc gatgcgtctg ctcttgcggc acggttttaa
     1201 caccctcaac ctaaaccgca tatttcttcg tgtattcgat accaatgccc gcgccatgcg
     1261 cacgtacgaa aaggcgggct ttgtccatga ggggcgcctt cggcaggcag aataccagga
     1321 tgggaagtac atcgatatgc tggtcatgag cgtgctatac tcagaatgga aagaaaatta
     1381 ggagcagggg ttcttatgct tactcactat cttcatcgag ataccaatat gtacggagac
     1441 atcaagctgt ttgcaggtac tgcctgccct gagttagctg gaacgattgc caagtatctc
     1501 gaattgcctc tcagcgggcg ggatgtcact ttattcccca acgacaatct ctttatccgg
     1561 ctgcacaaaa gcgttcgagg tcaggatgtg tatgtgatcc agaccacctc ccggcctgtt
     1621 caccgtaatt tgatggagct gctcatcatg ctccagacgc tgcgcctgga ctctgccggt
     1681 cgtgtgacag cggtcgtccc atacatgtgc tatgcccgct ccgataagaa agataaaccg
     1741 cgcgtgccga tcactgcacg tctggtcgcc gatatgatcg agatcgccgg agcagaccgc
     1801 tatatgacgc tcgatctgca tgctggacag atccagggct ttttctccat ccccggtgat
     1861 gtgttgaccg ctttccatat tctcactgct catgtcgaga agatgcttcc agcactcaag
     1921 gacccagtag tggttgcagc ggatctgggg tttgccaaga agggacgcaa cttcgctgcc
     1981 actattcagg cgccgattgc cttcattgag aagcggcgca gtgctaagga agaccagcca
     2041 atcgccctga ccatcatcgg tgacgttaaa gatcgagatg tgatcatcat cgacgatgag
     2101 gtagatactg ctagatcggt cgagcaggcg gtcaatctgg tcaaagctca tggggcgagg
     2161 actgtct
//
LOCUS       DBNHOH010000708         2809 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_748618, whole genome shotgun sequence.
ACCESSION   DBNHOH010000708 DBNHOH010000000
VERSION     DBNHOH010000708.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_748618"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="AC16V7_12325"
     CDS             complement(<1..673)
                     /locus_tag="AC16V7_12325"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR6257952.1"
                     /translation="MSHEFVQFELERLMSIWENRVDYNLSESGVHPLSIKELVEDPQF
                     LDELLSTPLNYPQANGLVELRQRVASLYPGATPDNVLITTGCAQANFTSLLTIMAPGD
                     EIVVMLPNYMQIWGVAQNFGYQVKTFSLKEELDWGIDIDELNAVVSSKTKLIAIVNPN
                     NPTGHIMTTDEMDAVVAVAQRSGAWLLCDEVYAGAERVTEQVTPSFWGRYERVLATGG
                     MSKAYG"
     gene            complement(728..1717)
                     /locus_tag="AC16V7_12330"
     CDS             complement(728..1717)
                     /locus_tag="AC16V7_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase"
                     /protein_id="HIR6257953.1"
                     /translation="MKVTILNEAEIRSCVTLEEEAIEAVADGFSRLANQQASLPAVIR
                     VDVPENHGEVDIKTAYIHGLDHFAVKIASGFYDNSLSGLPYGSGLMIILSTITGFVEA
                     LLLDNAYLTDLRTGIAGAIAARHLARDPIEGAGVIGSGTQARYQIKALKLVRDFKRLY
                     VYSIDPQHVEEYAREMKQTLGVEVITADRPETVVRMSDVVVTTTPSHQPYLKAEWLHP
                     GLHITCMGSDTEHKQELYADVLGRADRIVCDRIAQCAQLGELHHAIEAGVISVGDQIS
                     ELGDLTSGRRPGRQSEDEITVCDLTGVGVQDTAIALLAYQRALQKGLGTSIEV"
     gene            complement(1780..>2809)
                     /locus_tag="AC16V7_12335"
     CDS             complement(1780..>2809)
                     /locus_tag="AC16V7_12335"
                     /inference="COORDINATES: protein motif:HMM:NF018012.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR6257954.1"
                     /translation="LNIDFVMSMFLPADVDQTIADTYQAEVMLSHTTKPIILVSYGAS
                     GLRNAVEMAEVVAGGPEALRAHPLLTCYINVVSGAVHNEECLEKLLYLAEKGLPSIYI
                     PGSNAGVTSPISMPGAVALDMAGGLLGLVLSQLKREGTPYILSAMDPAALDMRTSVSP
                     YAYAERGIIRSVSQRYGLPTFSLSGCSDSKVVDQQAAAEAALTMMAEVLMGGNIVHDL
                     GYLESGLTFSFTQLAICDQMANWVKAFFSPVEVNDETLALNVITEVGPGGQYLKHKHT
                     RAHFREHWYPDLFERGNYTDWAQKGSQTLGERAATRVQKILDEHHPQPLPDAVRARLR
                     QIVQQAAA"
BASE COUNT          599 a          784 c          758 g          668 t
ORIGIN      
        1 atccataggc tttcgacatg ccgcctgtcg ccagcacgcg ttcgtaccgc ccccagaagg
       61 aaggggtgac ttgctctgta accctctccg ctccagcgta cacctcgtcg cacaacagcc
      121 aggctccgct gcgttgggcc accgcgacga ctgcatccat ctcatccgtg gtcatgatgt
      181 gcccggtggg attgttcggg ttgacaatgg cgatcagctt ggtcttgctg gacactacag
      241 cgtttagttc atcgatatca ataccccagt ccagctcttc tttcagtgag aacgtcttca
      301 cctgatagcc aaaattttgt gcaacccccc agatttgcat atagttgggc agcatcacca
      361 caatctcatc accgggtgcc atgatcgtta acagcgaggt gaagttcgcc tgagcgcaac
      421 cggtggtgat cagcacattg tcgggcgtag cgcccgggta gagcgaagcg acccgttggc
      481 gcaactcaac caggccattg gcctgcggat aattcagggg tgtggagagc agttcgtcga
      541 gaaattgagg gtcttccacc agttccttaa tgctcaaagg gtggacgccg ctctctgaga
      601 gattgtaatc gacgcggttc tcccaaattg acatcaggcg ttcgagttca aattgcacaa
      661 attcatggct catctgtgcc tcctatcgat ggtatttcta tccagagtgc agatggtagg
      721 cgggtagcta aacttctatg cttgttccca gccccttttg caaggctctt tggtatgcca
      781 ggagagcgat ggcagtatct tgcacgccca caccggtcag gtcgcaaacc gtaatctcat
      841 cttcactctg ccgtcctggc ctccgaccgg aggtcaaatc tcccagctca gagatctgat
      901 ctccgactga gatgacgccc gcctcaatag catggtgcag ctcgcccaat tgggcgcatt
      961 gtgcgatgcg gtcgcatacg atgcggtcgg ctcgacccag cacatctgca tataattcct
     1021 gcttgtgctc agtatcggat cccatgcagg tgatatgcag cccgggatgc aaccactccg
     1081 ctttcaggta tggctggtgc gagggagtgg ttgtcaccac cacgtcgctc atcctaacca
     1141 cggtctcggg tcggtctgcg gtgataactt caacgcccaa ggtctgtttc atttctcgcg
     1201 catattcttc gacatgttgc ggatcgatgc tataaacata caggcgcttg aagtccctca
     1261 ccagcttcaa ggctttgatt tgatagcgtg cttgagttcc tgagccgatg acccccgcgc
     1321 cttcgattgg gtctcgcgcc aggtgccggg ctgcgatggc gccggcaata cccgtccgta
     1381 gatcagtcag ataagcgttg tccagcagga gcgcctccac aaagccggta attgtgctta
     1441 atatgatcat caatccgctg ccatagggta atcctgaaag ggagttgtcg tagaacccgg
     1501 aagcgatttt aacggcgaag tggtcaagcc cgtggatata ggcggtcttg atatcgacct
     1561 cgccatgatt ttcggggaca tcgacgcgga tcaccgctgg caggctggct tgttggtttg
     1621 ccaagcggga aaacccatcc gcgacggcct cgatcgcctc ttcttccaag gtcacacaac
     1681 tgcggatttc cgcttcattt aaaattgtga ctttcattat tttctacctc cgatgcaggt
     1741 cgcggatacc ttttgttcgg atggtatcct tgcgaccggc taggctgcgg cttgctggac
     1801 gatttggcgt aaccttgctc gcacagcgtc tggtaaaggc tgtggatggt gctcgtccaa
     1861 tatcttttgt acgcgggtag ctgcccgctc accgagcgtt tggctccctt tttgagccca
     1921 atccgtgtag ttgccgcgtt cgaataaatc tgggtaccag tgttcgcgga aatgtgccct
     1981 ggtgtgcttg tgcttcaggt actggcctcc tggaccgact tcggtgatta cattgagcgc
     2041 aagtgtctca tcattgacct caaccgggct aaaaaacgcc ttgacccagt tggccatctg
     2101 atcgcaaata gccagctgag taaatgaaaa cgtaagacca gactccaaat agcccaggtc
     2161 gtggacaatg ttgccgccca tcaacacttc tgccatcatg gtcagggccg cctccgcagc
     2221 ggcttgttgg tcgacaacct tggaatcgga gcagcctgat aaggaaaagg tgggcaaacc
     2281 ataacgttgc gacacagagc ggatgatgcc gcgctcggca taggcgtacg gcgagacaga
     2341 agtgcgcata tctagggcag ccgggtccat agcggacaaa atgtaaggcg tgccctcacg
     2401 cttgagctgc gagagcacca atcccaataa gccaccagcc atgtccaagg cgaccgcgcc
     2461 gggcatgctg atcgggctgg tcacaccggc gtttgagccg ggaatatata tggatggcaa
     2521 tcctttttct gctagataga gcagtttctc caaacattcc tcgttgtgca ctgcgccgga
     2581 aactacattt atatagcagg tcaagagagg atgagcgcgt aacgcctcgg gaccacccgc
     2641 caccacctcg gccatttcta cggcgttcct caggccgctt gccccgtaag agaccaggat
     2701 gatcggcttg gtggtgtgtg acagcatgac ctccgcctgg taggtgtcgg cgatggtctg
     2761 atctacatcg gctggcagga acatcgacat gacaaaatct atattgagt
//
LOCUS       DBNHOH010000709         2817 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_235611, whole genome shotgun sequence.
ACCESSION   DBNHOH010000709 DBNHOH010000000
VERSION     DBNHOH010000709.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_235611"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..2634
                     /locus_tag="AC16V7_12340"
     CDS             286..2634
                     /locus_tag="AC16V7_12340"
                     /inference="COORDINATES: protein motif:HMM:NF013124.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR6257955.1"
                     /translation="MNPNPRKPSKQRSSLPIQLTTFIGRQRELNEISALLEDQFTHLL
                     TLSGPGGVGKTRLALQAAALVQDQFKDGVFFVDLAPLQDPNLVINRIAHELGVKESLT
                     HALLNDIIDHLSTKHSLLLLDNFEQILEAASTVSELIANVPSLKVLVTSREALHLYGE
                     QEYPVLPLSLPERRADISHDRFSEYEAVDLFIQRAQASNPNFRLSEINARHVAELCIH
                     LDGLPLAIELAAARVKIFSPEYMLDLLSNSLGMLTAGPRDFEARHQTLRAAIDWSYNL
                     LDEDEKTLFARLGIFQGGHSLEAIEIVCGHTLESGVLHCLESLLNKSLIIQKVGPKGE
                     PRFVMLETIQQYARERLKQSGEVEQMHRRHAEYFAELAKQSEPLLRGPKQEYWSARLR
                     ADYDNLLAALEWSFAQADASIGLRLAASLSEFWYYEGSISQSRKWIEVALKRVDQAPP
                     EIHAKMLNGASMLAFVFGQHERGKQLNQEALAIARQSGDRYNQACALLWLSAHATKNP
                     NEYLESVQFSEQAVALFEELGDQAGLAWGYNQIGESTRLVGDFNRAGRAYEASLAVCR
                     QTGNRRREAISLVNLSYVAQRQGEYEKAENYVIKGLELLHSLDLRYHCAIALAMLSGP
                     VASQGKAWRAAKLLGASQAIFERMSTGLQPADQVEIDGYAAAAAKQLDPQDYQAAWAE
                     GQRLSFEQAMMYAPQKDLDAKRGTASMSNALTVKIMGEKLSARELEVLCQLASGLSIE
                     EIAAKLYISPSTLRSHTKNIYRKLDVHRRAEAVQRGKELGLL"
BASE COUNT          703 a          751 c          759 g          604 t
ORIGIN      
        1 gaatcatcag tgcgtcctgc ggttggggag tgtatttggg gttagcaaga gtatacccta
       61 cctatccaga ttaagtcaat cttatttatc gaaatttacc aggctttaac catatcggga
      121 aagccaatca ttatccatgg ctacgagtat gtatatgtag acattattgc acacagtggg
      181 gtacaatcta attatcgaat catcttcttt gattacaaat attgtcctgg cacaaagaag
      241 acttcaaacc tgcacccgca tggaataacc agaggttttc gactggtgaa tcccaatcca
      301 cgcaagccct ccaagcagcg cagctccctg cccattcaac ttactacttt catcggccgc
      361 cagcgcgagc ttaacgagat tagcgctctg ctcgaagacc agtttacaca cctgctgaca
      421 ttgagcggtc ccggcggggt gggcaagacg cgcctggctc ttcaggctgc cgcgctggtg
      481 caagaccaat tcaaggatgg cgtattcttc gtcgacctgg caccattgca ggatccaaac
      541 ctagtcatca accgtatcgc ccatgaatta ggcgtcaaag agtcgttgac gcacgctctg
      601 ctaaatgaca tcatagatca cctaagcaca aagcacagcc tgctcttact ggataacttc
      661 gagcaaatac tagaggctgc cagcacggtt agtgaattga tcgctaacgt gcccagcctg
      721 aaggttctgg tgaccagccg agaggcacta cacctgtatg gcgaacagga ataccctgtg
      781 ctgccgctgt cattaccaga gagacgggct gatatctccc atgatcgttt ctctgagtat
      841 gaagcggtgg atctattcat ccagcgcgcc caggcctcta acccgaactt ccggctttcc
      901 gagatcaacg cccgccatgt ggctgaactg tgcatccacc tggacggcct gcccctggcg
      961 atcgaactgg cagccgccag agtgaagata ttctcgcccg aatatatgct tgacctgcta
     1021 agcaattcgc ttgggatgct caccgcgggg ccgcgcgatt tcgaggcccg acaccagaca
     1081 ctgcgagctg ccatcgactg gagttacaat ctgctcgacg aggatgagaa gacgctgttt
     1141 gcacgcctgg gtatcttcca gggcggccac agcctggaag caattgaaat cgtttgtggt
     1201 cacactttgg aaagcggtgt gctgcattgt ctagaatccc tgctgaataa gagcctgatc
     1261 atacagaaag tagggcccaa gggcgaaccg cgcttcgtca tgctggaaac catccagcag
     1321 tatgcccgcg agcgcctgaa gcaaagcgga gaggtggagc aaatgcacag gcggcatgct
     1381 gaatattttg cagaacttgc caagcaatcc gagccgcttt tgcgaggccc caaacaggaa
     1441 tactggtcgg cacgtctcag agcagattat gacaacctgc tggctgccct ggaatggtcg
     1501 ttcgcccaag cagatgcaag cattgggttg cgcctggcag catccttgtc tgaattttgg
     1561 tactacgaag gctccatttc tcagagtcgg aaatggatag aggtagcgct caaacgggtg
     1621 gatcaggcac ccccagagat tcacgccaag atgctgaacg gagctagtat gttggctttt
     1681 gtttttgggc agcacgagcg cggcaagcag ttgaaccagg aagcgttggc tatcgcccga
     1741 cagagtgggg accgctacaa ccaggcgtgc gcgctcttat ggctcagcgc ccatgccacc
     1801 aaaaacccaa atgaatacct ggagagcgtt caattctctg agcaggctgt tgccctgttc
     1861 gaggagctcg gtgatcaagc cggcttagct tggggatata accaaatcgg agagtcgacc
     1921 cgcctggtgg gagatttcaa tcgggctggc agggcttatg aagcttcact ggctgtctgc
     1981 cgccagacgg gcaaccgtcg ccgggaggcg atctccctgg taaacctgag ctatgtggcc
     2041 cagcgccagg gtgaatatga aaaagcggaa aattacgtca tcaaagggct ggaattgtta
     2101 catagcctgg accttaggta ccactgcgct atcgcgctgg cgatgctgtc cggcccagta
     2161 gcctcgcaag gcaaagcctg gcgggcggcg aagctgctcg gcgcgtcaca ggccattttt
     2221 gagcgaatgt cgaccggcct gcagccagcg gatcaagtcg agatcgacgg ctatgccgcg
     2281 gctgcagcca aacagctgga tcctcaagat taccaggcag cctgggcaga aggacaacgg
     2341 ctttcctttg agcaagccat gatgtacgcc cctcaaaagg acctggatgc aaagcggggc
     2401 acagcatcca tgagcaatgc gctgacggtg aaaattatgg gggaaaaact cagcgcgcgg
     2461 gagctggaag tgctatgcca actggcatct ggtctctcca tcgaagagat tgccgcaaaa
     2521 ttatatatct cgcctagcac actgcgctcc cacaccaaga atatctatcg aaaactcgat
     2581 gtgcaccggc gtgcggaggc agtgcaacgc ggcaaggaac tgggcttgct gtaacccccc
     2641 aaataaagta gcaccaaata cccaaataca tccccaaaat tggggatgac accgtcccca
     2701 ttaattcgat atgatcaggg cagatatggc gttccatata gttaatgata tggatcgtgc
     2761 aagtgttttt tgatagtgaa gccgcagaac atcaaattgg gtatcccggt cggtgtt
//
LOCUS       DBNHOH010000710         4757 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_235666, whole genome shotgun sequence.
ACCESSION   DBNHOH010000710 DBNHOH010000000
VERSION     DBNHOH010000710.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_235666"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="AC16V7_12345"
     CDS             complement(<1..244)
                     /locus_tag="AC16V7_12345"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HIR6257956.1"
                     /translation="MQNDDLETKERLLNAAIELLGEEKQIEKITSRLIAERAEVSLGS
                     INYHFQSKANLLNEAVGRMTYEIAESWLEPVQGFDDD"
     gene            400..1299
                     /locus_tag="AC16V7_12350"
     CDS             400..1299
                     /locus_tag="AC16V7_12350"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR6257957.1"
                     /translation="MRAQVSRVAELRASIIDEIFNAMGLPRGWLRQALWPLAWAPAHR
                     FARLVEAFERCNAEYGFSEAARCLLERFIHHVVVSGAEHIPPEGPLLIASNHPGAVDG
                     LAIAASLPRQDLKIVVSGVPFFHNLSFIQEHLIYTAENAHGRMLVLRNAIRHLRQGGA
                     VLIFPSGLVDPDPSLFSDAWQSLQTWSSSLALMLEKAPSTRLVITAVSGVLAPECWRH
                     PLVRLRQKMWEKQKLAEFIQVIQQLVLGRRYPIVPRVSFAEPLTLSELQLGEKDGDTM
                     QAVRARARRVMEAHLSLQGEVYR"
     gene            complement(1461..1928)
                     /locus_tag="AC16V7_12355"
     CDS             complement(1461..1928)
                     /locus_tag="AC16V7_12355"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HIR6257958.1"
                     /translation="MGSQSQRVATPYDELTYRIIGCAMAIHRKLGPGYRENTYQRDLE
                     AHFTDTGLAFEGQKLLEVYDSSKGEQLIGYYIPDFLVENTVVVEIKALRGLDNSHLAQ
                     VIGYLAVTGCPIGLLINFGTRSLQYRRIFPPRDITEHRVNQQWLFVPDWLKNN"
     gene            complement(1988..4405)
                     /locus_tag="AC16V7_12360"
     CDS             complement(1988..4405)
                     /locus_tag="AC16V7_12360"
                     /inference="COORDINATES: protein motif:HMM:NF013727.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIR6257959.1"
                     /translation="MARQTRKDGRKKPDKSSRPAVKSQKSKPNPGRGASNKKPGRTSK
                     PAARGSSSRQSRQAPGKPASRSKSTPSPGQSFTSGLSLDRKLDIAGVVLSLLGLLTLL
                     SLISSSRSAITSGWIGMLSKGLGWGIYLLPLTLLVVGLWLVLRNFEHIPLPSPERLLG
                     LSLLYLNLLGILHLFKLPETREASLALADAGQGGGYLGALTLEILRTILGMGGATVAL
                     LAWLLIAMALSLDVSVVELFHWLPPLLSRLQDRWNDFRLQRLSKGVSQPELDGFTPLQ
                     RGVDLDYELDPQIANRLAALPSIPPTLGVTPKDWQLPVVEEILEAGGEVSYDTELDKQ
                     RGRVIEETLASFGAPAKVVEISRGPTITQFGVEPDFIESRGGRMRVRVGKIAALADDL
                     ALALSASTIRIQAPVPGKGYVGIEVPNDEISLVTLRSVIENEAFRRVKSPLGFALGQD
                     VAGNAVAADLAAMPHLLVAGATGSGKSVCVNSLITCLLLHNTPEDVRLIMVDPKRVEL
                     TGYNGIPHLLAPVVVELERVVGALQWVLREMDQRYHKLAQAGCRNLKEYNAKLVARGE
                     KKLPFLVVFIDELADLMMLAPDETERTITRLAQLARATGIHLVIATQRPSVDVVTGLI
                     KANFPARIAFAVASGVDSRVILDQPGAERLLGRGDMLFQAPDAAAPVRLQGVFVSDSE
                     IQRLVQYWQSFAGAVTLSPTAVGGVVDAVPSGLPLKQIPLWDDFTPEEEKDPLLDEAI
                     DLTRRQGRASVSMLQRRLRIGYTRAARLIESMEAKGIVGPPESGTGAREVLDYGPAAP
                     PIDEQRE"
     gene            4507..>4757
                     /locus_tag="AC16V7_12365"
     CDS             4507..>4757
                     /locus_tag="AC16V7_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6257960.1"
                     /translation="MFEIIFLGTSASAPSVHRGLSAQVVMHDEYRFLIDCGEGTQRQI
                     LQSGIGFKRLSRILITHGHLDHILGLAGLLSTFSRWEAI"
BASE COUNT          965 a         1316 c         1429 g         1047 t
ORIGIN      
        1 gatcgtcatc gaagccctgc accggctcga gccagctttc agcgatctcg taggtcatcc
       61 ggcccacggc ttcgttgagc aggttcgcct tgctctggaa atggtaatta atggagccca
      121 ggctgacttc ggcgcgctcc gctatcaggc gggaggtgat tttttcaatt tgtttttcct
      181 cgccgagcag ctcgatggcg gcgttgagca ggcgttcttt cgtctctaga tcatcatttt
      241 gcatggtata tatcccggtc gctttgaaca gtattagaac aaatattgaa catgttcaaa
      301 ttatacatta tttaatagaa ttgtcaagag ggaattttaa agatcgtggt gtacaataat
      361 caagaagtag cagtggcggg gtaaacagca ggagatgcca tgcgagcaca ggtctcccga
      421 gtagcggagc taagagcctc tatcattgac gagatcttta atgccatggg tttgccgcga
      481 ggatggctgc ggcaagctct gtggcctttg gcttgggcgc cagcacaccg ctttgccagg
      541 ctggtggaag cctttgagcg ctgcaacgcg gagtatggtt tcagcgaagc ggcgcgctgc
      601 ctgctggagc gctttatcca tcatgtggtg gtgagcggtg ctgagcatat cccccccgag
      661 ggcccgctgc tgatcgcctc caaccatcca ggtgcggtgg acggtctggc aatcgctgcc
      721 agcctgcctc gccaggactt aaagatcgtg gtcagcggtg tgccgttctt ccacaatttg
      781 tctttcatcc aagagcacct gatttacacg gctgagaatg cacacgggcg catgctggtg
      841 ctgcgcaatg ccatccgcca cctacgccag ggtggggcgg tgttaatctt ccccagcggt
      901 ctcgtagacc ccgatccatc cctcttctcc gatgcgtggc agtccttgca gacctggtcg
      961 tccagtttgg cgctcatgct ggaaaaagca cctagcacgc ggctggtgat cacggcggtg
     1021 agcggtgtgc tggctcctga gtgctggcgg cacccactgg tgcgcttgcg ccagaagatg
     1081 tgggaaaagc agaagctggc ggagtttatc caggtgatcc agcagcttgt cttggggcgg
     1141 cgttacccga ttgtgccccg ggtgagcttt gccgagccgc tcaccctatc tgagctgcaa
     1201 cttggtgaaa aggatggaga taccatgcag gctgtccggg ctcgcgcccg gagggtgatg
     1261 gaagcacact tgtcactcca gggtgaggtt tataggtgat ctttacgctt ccccgaggga
     1321 ttatttacga gatcgtgata acggattact gtaaacggat ggcgggaacg gattgaacgg
     1381 atggctggcg acggacaatg ggaacggatg gctgtgaacg gatttttaga gcggattgaa
     1441 cggattacct gcctgatggt ttaattgttt ttgagccaat ctggcacgaa caaccattgt
     1501 tgattaacac ggtgttcggt gatatccctt ggagggaaaa tacgccggta ttgcaagctg
     1561 cgcgtgccga agtttatcag caggccaatt gggcagccag tcacggccag gtaaccgatc
     1621 acctgggcga gatgactgtt atccagtcct ctcaaggcct tgatctccac cacgactgta
     1681 ttctcgacga gaaaatccgg gatatagtaa ccgattaatt gctcgccctt cgagctatca
     1741 tatacttcga gtagtttctg tccctcaaaa gcaagcccag tgtcagtgaa gtgcgcttcc
     1801 aggtcccttt gataggtgtt ttcacggtac ccgggtccca gtttgcgatg tatcgccatg
     1861 gcacacccaa tgatcctata ggtcagttca tcatacggtg ttgcgacacg ctggctttgg
     1921 gaccccatgt gattacctct ttaatgcacg atccgtgtaa tccgttcccc aaatccgtta
     1981 tcagcaccta ttcgcgctgc tcatcaatcg gaggcgcggc tgggccgtag tccagcactt
     2041 cacgcgcgcc tgtgccgctt tcgggcgggc caacgatacc tttagcctcc atgctctcga
     2101 tgaggcgggc ggcgcgcgtg taaccgatgc gcaagcggcg ctgtagcata gagacggagg
     2161 cgcggccctg gcggcgggtc aggtcgatgg cctcgtccag cagggggtct ttctcctcct
     2221 ctggagtgaa atcatcccat aagggaattt gcttaagtgg caaccccgag ggcacggcat
     2281 ccaccacgcc cccaacggca gtgggggaga gcgttactgc gccagcgaag ctctgccagt
     2341 actgcaccag acgctggatt tcgctatctg agacgaagac accttgcaag cgaaccggcg
     2401 cggcagcatc gggggcttga aagagcatgt cgccgcgccc cagcaagcgc tcagcacctg
     2461 gctggtcgag gatgacgcgg ctatccacgc cggaggccac ggcgaaggca atgcgcgccg
     2521 ggaaattggc tttgatcagc ccggtgacta cgtccactga gggccgctgg gtggcaatca
     2581 ccaggtggat gcccgtggcg cgggccagtt gtgccagacg agtgatggtg cgctcggtct
     2641 catctggggc aagcatcatc aagtcggcta attcatcgat gaacaccacc aggaaaggca
     2701 gcttcttctc accccgcgcc accagcttgg cgttgtattc cttgaggttg cggcaaccag
     2761 cctgggctag cttgtggtag cgctggtcca tctcgcgcag cacccattgc agcgcgccca
     2821 ccactcgctc cagctccacc acgacaggtg ccagcaggtg ggggatgccg ttatatccgg
     2881 tcagctcaac ccgcttggga tcgaccataa tcaagcgcac atcttctggg gtgttgtgca
     2941 gcagcaggca ggtgatcaac gagttgacgc ataccgactt gcccgagccg gtagccccag
     3001 cgaccagcag gtggggcatg gcagctaggt cagccgcgac ggcgttacca gccacatcct
     3061 gtcccaaggc gaaacccagg ggtgatttga cccgccggaa ggcttcgttc tcgatcacac
     3121 tgcgtagcgt caccaggctt atttcatcgt tgggcacttc gatgccaacg tagcccttgc
     3181 cgggtacggg tgcctggatg cggatggtgg aagcggaaag ggccagcgcc aggtcgtcgg
     3241 ccagggcagc gattttgccc acgcgcacgc gcatgcgccc gccgcgcgac tcgataaaat
     3301 cgggctccac gccgaactgc gtgatggtgg gaccgcggct gatctctacc actttggctg
     3361 gcgctccgaa ggaggctagt gtctcttcaa ttaccctgcc gcgctgtttg tccaactccg
     3421 tgtcatagct gacctcgcct ccggcttcta agatctcctc aacgacaggg agctgccagt
     3481 ctttgggggt gactcccagg gtgggaggga tggatggcaa cgctgccagg cgattagcaa
     3541 tttggggatc gagctcgtaa tccaaatcca caccgcgctg caggggggta aaaccgtcta
     3601 gttcgggttg tgaaacacct ttggacaggc gttggagacg gaaatcattc catcgatctt
     3661 gcagccggga gagcaagggt ggcagccaat ggaaaagttc taccaccgaa acatccagtg
     3721 acaacgccat ggcgattaat aaccaggcca ggagggctac tgttgccccg cccatgccta
     3781 agatcgtgcg caatatctcc aaagtcagcg cgcccaggta gcctccgcct tgccctgcat
     3841 cggccagagc caaggaggct tctcgtgtct ctggaagctt gaacaggtgc aggatgccca
     3901 gcaagttcag ataaagcagg ctcagcccaa gcaggcgctc aggggatggc aaggggatgt
     3961 gctcgaagtt gcgtagcacc agccaaagtc ccaccaccag caaggtcagc ggcagaaggt
     4021 aaatgcccca tccaagtcct ttgctgagca tgccgatcca gccgcttgtg atggcagagc
     4081 ggctgctgga gatcaggctg agcagggtaa gcaagcccag cagggatagc actaccccag
     4141 cgatatccag cttgcgatct agggaaagtc cgctggtgaa ggactgccca gggctgggtg
     4201 ttgacttgga acgggaggct ggtttgccag gagcctgacg ggattgacgc gacgaactgc
     4261 cccgggcggc tggtttgctg gtgcgccccg gttttttgtt cgaggcgccg cggcctgggt
     4321 ttggcttcga tttttgggat ttgaccgcag gcctggaaga cttatcaggc tttttgcggc
     4381 cgtcttttcg ggtctggcgg gccatgacga taaaaattat agcacatatg gtcgtattat
     4441 tgcgtgaaac acggtcgctc tattgcttgc cataagcgtg cctttgcggc taaaatagat
     4501 gagcctatgt ttgagattat tttcctcggt acgtcagcgt ccgccccctc ggtgcaccgc
     4561 ggcctttcgg cacaggtggt gatgcatgac gaataccgtt ttctgatcga ctgtggtgag
     4621 ggcacccagc gccagatact gcagtctggg atcggcttta aacgcctgag ccgcatcctg
     4681 atcacccacg gtcacctgga tcacatcctt gggttggcag gtctgctgtc caccttctct
     4741 cgctgggagg cgatcga
//
LOCUS       DBNHOH010000711         2085 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_555118, whole genome shotgun sequence.
ACCESSION   DBNHOH010000711 DBNHOH010000000
VERSION     DBNHOH010000711.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_555118"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1463)
                     /locus_tag="AC16V7_12370"
     CDS             complement(<1..1463)
                     /locus_tag="AC16V7_12370"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6257961.1"
                     /translation="MRWIVSHSIRYWYLLVSMLVGAIGNAALAAVVPVIIGEAINSVR
                     NTPFEQSVLLRMAFWIALSQVIRAVLQLARNFSAELIGQRLERDIRDELYVGLLGKSM
                     TFHSLQPVGDTMARATNDVREINLMFSPGINLVIGSASFLIMPLFLAPRYHPSLIITP
                     ALFMIGYFLSLWQYLRELDPVTGEVRKSFGHLNSRLAEALDGIETVKGMVQENSEVGL
                     FTRYARGYRDAFIRQIDIEARFLPLLLLGLAEAGGLLHAILLFWQGQLDVGAVVAYFG
                     LLQLFGFPTFVSLFSYSQVSLGIASARRILELINRENNLDQNVSGYSGSMRGEIEFRN
                     VTFSYGDEEPVLHEVSFKVQAGQTVAIVGQTGAGKTSLVRLVNRTYDVAGGQVLVDGI
                     DVRQWNLEALRRNISMIEQDIFLFSRSIADNVAFGKQEASMQSIIQAANAAQANDFIE
                     SFKDSYQTVIGERGVTLSGGQRQRLALARAFLTDPRI"
     gene            complement(1597..>2085)
                     /locus_tag="AC16V7_12375"
     CDS             complement(1597..>2085)
                     /locus_tag="AC16V7_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257962.1"
                     /translation="NQPQRPGRILSDVPDGQVMIECQGHKLCQRNFRSITCRSFPFFP
                     YITRQDEFIGMTYYWAYADRCWVISHLSEVRFDYVNEFISTFEALFERMPDDRENFRH
                     YSSVMRRHYGRLKKAIPLLHRNGKYYKITPRNGRKRRVSPESFPKFDPYRIADRLPFA
                     DE"
BASE COUNT          557 a          500 c          534 g          494 t
ORIGIN      
        1 aagatgcgtg gatcggtgag gaaggctcgc gccaacgcca ggcgctgtcg ctgaccaccc
       61 gagagagtaa cgccacgttc gccgataacg gtctggtagc tatctttgaa cgattcgata
      121 aaatcatttg cctgagctgc attggcagcc tggatgatcg attgcatcga ggcttcttgc
      181 tttccaaagg ctacgttatc agcgattgag cgtgagaaaa ggaatatatc ctgttctatc
      241 atggatatat tgcgtctcaa cgcctccagg ttccactgcc gcacatctat tccgtctaca
      301 agtacttgac cgcccgccac atcataggtt cggttaacca gcctgacaag gctagtcttt
      361 ccagcgccgg tctggcctac tattgctact gtttgccctg cttgaacttt aaaagagacc
      421 tcgtggagaa ctggttcttc atcgccgtaa gaaaatgtca cattgcgaaa ttcaatctct
      481 cctcgcattg aacctgagta gccgcttaca ttttggtcta ggttgttctc acgattaata
      541 agctctaata tgcggcgtgc gcttgctatt cccaatgaga cctgggaata ggagaaaagc
      601 gagacaaagg ttgggaagcc aaagagttgg agaagtccaa aataggcgac cactgcaccc
      661 acatctagtt gcccttgcca aaatagcagg atggcatgta ataagccacc agcttcggcc
      721 agacctaaca ataaaagtgg taaaaagcgg gcttcgatat cgatctggcg gatgaatgca
      781 tcccgatacc cgcgggcgta gcgcgtgaaa agacctacct cgctgttctc ctggaccatc
      841 cctttaacgg tctcaattcc atccaaggct tcagccagtc ggctatttaa atgcccaaat
      901 gatttccgaa cttcaccagt gacgggatct aattcgcgga ggtactgcca caaggagaga
      961 aagtatccga tcataaacaa ggctggtgta atgatcaggg aagggtgata acgcggggcg
     1021 aggaatagcg gcataataag aaaactagcc gagccaatca ccaggttgat gcctgggctg
     1081 aacataagat tgatttcgcg gacatcgttg gttgcccttg ccatggtgtc gccaactggc
     1141 tgcaggctat ggaatgtcat gctttttccc aataggccga cataaagctc atcgcggata
     1201 tcgcgctcta agcgttgacc aatcaactca gcgctgaaat tacgagccag ttgcaacacc
     1261 gcccgtatga cttgtgaaag agcgatccaa aacgccatac gcaataacac agattgctca
     1321 aagggtgtat tgcgtacaga gttgatcgcc tcaccaatta tgactggtac aacggcagcc
     1381 agcgctgcat ttccaattgc gccgaccagc atagagacaa gcagatacca atagcgtatg
     1441 ctatgtgaaa caatccaacg catcggacct cgacggttgg tgggatggcg tcggggtagg
     1501 ctaaattcgg ttacggaagg tgagttagct attgaggatt gcaatttaat tcatcagctg
     1561 agtgccggcg gcacggggta agtttatcta ggcgggtcat tcgtcagcaa atggcaatct
     1621 atcagcaatg cgataagggt cgaattttgg gaaactctct ggagagacac gccgcttacg
     1681 gccattacgc ggcgtaatct tataatattt cccgttacga tggagaagag ggattgcctt
     1741 tttcaaacgg ccgtagtggc ggcgcataac tgaagagtag tggcggaaat tttccctgtc
     1801 atctggcatc cgctcgaaaa gggcctcgaa ggttgagata aactcattta cataatcgaa
     1861 ccggacttcg gataagtggc tgatcaccca gcatcgatcg gcataagccc aatagtatgt
     1921 catgccgata aattcatcct ggcgagtaat gtaaggaaaa aacgggaagc tcctgcaagt
     1981 aatcgagcgg aaatttcgct ggcataattt gtgcccctgg cactcgatca tgacttgtcc
     2041 atcagggaca tcagagagga tgcgcccagg tctttggggt tggtt
//
LOCUS       DBNHOH010000712         9486 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1518332, whole genome shotgun sequence.
ACCESSION   DBNHOH010000712 DBNHOH010000000
VERSION     DBNHOH010000712.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9486)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9486)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9486
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1518332"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..153
                     /locus_tag="AC16V7_12380"
                     /pseudo
     CDS             10..153
                     /locus_tag="AC16V7_12380"
                     /inference="COORDINATES: protein motif:HMM:NF024591.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding enzyme"
     gene            196..711
                     /locus_tag="AC16V7_12385"
     CDS             196..711
                     /locus_tag="AC16V7_12385"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIR6257963.1"
                     /translation="MRIRGRVWKFGKNIDTDAIIPARRCNTAIPSELALYCMEDADPH
                     FVQKMKRGDLIVADTNFGCGSSREVAPLTIKAAGISAVIAKSFARIFFRNAINIGLPI
                     LECPKAVDSIREGDEVEVEPTAGLIKNLTRNEVYQAEPFPDFLKSIIDKGGLLAYVEE
                     QLTLSKTGENQ"
     gene            708..1856
                     /locus_tag="AC16V7_12390"
     CDS             708..1856
                     /locus_tag="AC16V7_12390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6257964.1"
                     /translation="MSDFELTDEQREMREMAHKFAEKEIRPIAAEYDEREEVPWEVIQ
                     KATKAGFMSYFIPERYGGGGITDLFTHCLVDEEIFWGCAGIATILGGTSLCATPILLA
                     GTEEQKEKYLSRFCDLDQPKLGAFALTEPSAGSDPASMITQAHRDGKYYVLNGYKTFI
                     TNAGIADVYVIFATVDVSRGVRGVTAFIVDKDWEGVVPGKKEKKMGIRASHTATLALE
                     GVRVPVENRLGEEGEGFAIAMRTFEHTRTHIAIGAVGLARAAFEYALQYAQERVQFGK
                     PISHHQAVSFMLAEMATQIDAARLLAWRAAYLADQGVPCTKEASMAKAFAADMAMQAT
                     TDAVQILGGYGYMREYPVEKWMRDAKIMQIYEGTAQIQRMIIARFITN"
     gene            1875..2954
                     /locus_tag="AC16V7_12395"
     CDS             1875..2954
                     /locus_tag="AC16V7_12395"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR6257965.1"
                     /translation="MKTYNIGVIAGDGIGPEVAAEGLKVLRTVSAQEGFECNLVDYPY
                     SGTYYLKTKELVPERVIDEWRTLDAVYLGAIGHPDVEPGLVERSVILGLRFGLDLYIN
                     LRPIKLYADHLCPLKDKGPKDIDFVVVRENTEGLYSQIGGFLKKGTPDEVATVTGVYT
                     RKGVERAIRYAFELARQQGKARADQRQPRLTLVDKSNAIRPQDLWVRTFEEVGQEYPD
                     VEQDHAYVDACTMWMVKNPEWFDVIVTTNLFGDIITDLGAMLQGGMGIAASGNIHPGK
                     TSMFEPIHGSAPKYTGKNIACPLGAIMAVSMMLDFLGEKKAALSVESAVAHLLASGAI
                     PSADARSGLSTTQIGDMVVESLQKA"
     gene            3071..4585
                     /gene="accC"
                     /locus_tag="AC16V7_12400"
     CDS             3071..4585
                     /gene="accC"
                     /locus_tag="AC16V7_12400"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR6257966.1"
                     /translation="MFNKVLIANRGEIAVRIIRACRELGMRTVAVYSEADRQGLHVRY
                     ADEAYLLGPAPSRESYLRSDKIIEIARKCGAKAIHPGYGFLAERADFAQDCLDVGLAF
                     IGPKPSSIAAMGDKAVARATVAKAGVAVVPGTEGEGALGDEELLSVAPEIGFPLLIKA
                     TAGGGGKGMRVVHHLEEMHSLLHSARREAEASFGDGNVYLEKLVEGARHIEFQILADT
                     HGNVIHLGERECSLQRRHQKLLEESPSPFISDDDEMRQRMGNVAVRAAQAVGYVNAGT
                     IEFLVDKDKNFYFLEMNTRLQVEHPVTEMVTGVDIVKEQIRIARGRQMRYSQENINLN
                     GWAIECRINAEDAYNNFLPSTGQISHSLLPTGPGVRVDTGVYPGFEISPYYDSLISKL
                     IVWGETRAEAILRMRRALEEYKIVGVRTNIPFHQNLMDSHRFMGGQFDTRFVEERFSM
                     EAAEEGKSPRPEIAAIIATLVAHEQTQRAAHIIQRNERDTSNWKWISRWERMQR"
     gene            4613..5110
                     /locus_tag="AC16V7_12405"
     CDS             4613..5110
                     /locus_tag="AC16V7_12405"
                     /inference="COORDINATES: protein motif:HMM:NF012584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016421 - CoA carboxylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HIR6257967.1"
                     /translation="MKYVTTIGEREYIIEIVDERHIILDGTPFEVDFDSVSGQPVYSL
                     LLDGQSYESFVYSSEDGWEVLLHGTLYPASVEDEREKRLRAAAGGNIAEAGEFYLRAP
                     MPGLVISILVDEGQSVEKGDVLVILESMKMQNELKSPRPGTIARMRVKAGDSVEQKQT
                     LLSVV"
     gene            5248..5868
                     /locus_tag="AC16V7_12410"
     CDS             5248..5868
                     /locus_tag="AC16V7_12410"
                     /inference="COORDINATES: protein motif:HMM:NF014038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HIR6257968.1"
                     /translation="MKNQELEVKFFVADLLEIEARLRALSAHLEQSRVHEVNLRFDTP
                     DGDLGRGYRVLRLRQDDAARLTYKGPSQYQEGVRARQEIEFTVSDFEAARAFLEALGY
                     VVSMMYEKYRTTYELDGVHITLDEMPYGDFIEIEGPDPKSIRSIQDRLELDWEKRVPE
                     SYTALFEQLRLALRLPFKDLSFANFDGLDISMSVIRIQPADLNEQR"
     gene            5907..6491
                     /locus_tag="AC16V7_12415"
     CDS             5907..6491
                     /locus_tag="AC16V7_12415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015236502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR6257969.1"
                     /translation="MAKDFSLNVTPPNKLQRAEYACDDDWIRNFFLQAEVGHVATSWD
                     EQPFITPILFWYDPQHHEIYFHTNITGRLRANIEHQDKVCIEASRIGRALPANTALEF
                     ALQYESAVAFGRGRVISDDDEKLRTLYGLIEKYFPKLTPGVHDRPITDQELKRTAVYA
                     IAIESWSGKRNWPARAKQLPDWPPLGDEWFEEDN"
     gene            6488..7438
                     /locus_tag="AC16V7_12420"
     CDS             6488..7438
                     /locus_tag="AC16V7_12420"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6257970.1"
                     /translation="MNEAVVLSEPSSRLARGYLICLTATVIWSTTGIFIGYITQNFNM
                     PPLLLAFWRDLIVSATLLTTFTLLSRSMLRISKNNLAFLAWYGLALTFLNTSWTISVA
                     LNGAAVSTVLVYSSPAITALVGWRLYGESLDMAKISAIVLSILGAALVSGAYQPQVWQ
                     VNLIGVVAGLLSGVAMAMYSLMGKGASLRQINPWSTLSYTFASATLFLLIINLTSLGQ
                     AQGLSHSNLLWLGNTPTPWIVLLILAIGPTIGGYGLYMVSLGYLQASVANLIATLEPV
                     FTAILAYLLLAERLNSVQILGGILIFTGVVLLRMYEGRRK"
     gene            7435..8862
                     /locus_tag="AC16V7_12425"
     CDS             7435..8862
                     /locus_tag="AC16V7_12425"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020976.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HIR6257971.1"
                     /translation="MTELLLGPVIGGLTHDSAYLWGRADGPSVLHAWLGRNPDLSDAR
                     LAGTSLPLSSDTGFAGVAVTKELQPNSRYYYALTLSGSPPTPATEGVSSFTTAPSPGE
                     RLSFSFAFGSCFRPENADGGRIFDAIEEQRKADDLRFILLLGDQIYADAYQNNGIGKI
                     ACTLQEYRDVYAYTWSRPPLQRLLANLPAYMTLDDHEVDDDWRWVDSNRRWANIPWWD
                     KVSRWLKRRPLQERRLPVKRAQDALQAYWEHQGMHAPSYKLTPQLNQAGQYELDVKDP
                     GSLAYTFTFGAAAFFVLDTRSMRVRNSREKIMLGEGQWQALKTWLRAVKDDYPIKFLV
                     TSCSMLYPMWHDLPRDRWTGYPEERDRLLHFLAANGIDGVYLLAGDLHSSHAVYAELY
                     GPEGRSLPLWEFCSSPFEQDCNRWGRYTYKPLHTSPVKVSYRAFSVHQVNYGVVKVDF
                     SRKDRPNVTFQIHDQQGEKLEAISV"
     gene            complement(8865..>9486)
                     /locus_tag="AC16V7_12430"
     CDS             complement(8865..>9486)
                     /locus_tag="AC16V7_12430"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HIR6257972.1"
                     /translation="AKLVVVACNAASAAALQYLRQTFPQVPFVGMEPAVKPAAEGTQS
                     GVVGVLATPATFQGALYASVVERFAQDVILLQHTCPGLVAQIEAGNLDSTETRRILEN
                     ALHPMLARGIDTVVLGCTHYPFVIPLIQEIVGTSVRVVDPAPAVARQVGRVLEARGLP
                     HPDREEGEVIYLTTGNPEQLEALLPRLVGEEGLVDLLEWGKDGDLL"
BASE COUNT         2183 a         2603 c         2684 g         2016 t
ORIGIN      
        1 ccagatttaa tctttggtga attaacggtg gcttgtcttg ttgcccaacc cggacttgag
       61 atcagtagcg atgagttatt agccttttta gccgaccatc tggcagatta tgccttgccc
      121 gaccggatca tcttcttcga tgcgctcccc cgccggggga gtggattggt gagaagaaat
      181 tatctgcgag aaagggtgcg catacgtggg cgggtctgga aatttggaaa aaacattgat
      241 actgatgcga ttatccccgc ccggcgctgc aacacagcta tccccagcga gctggccttg
      301 tactgcatgg aagacgctga tccccacttc gtgcagaaga tgaaacgcgg tgatctgatc
      361 gtcgctgaca cgaattttgg gtgcggatct tcgcgcgagg tggcgcctct gaccatcaaa
      421 gccgcgggga tctcggcagt gatcgcgaaa tctttcgccc gcattttctt ccgcaacgcc
      481 atcaacatcg gcctgccgat cctggaatgc cctaaggcgg tcgacagcat ccgagaaggc
      541 gacgaggtgg aagttgagcc cacggcaggc ttgatcaaga acctgacccg caacgaggtc
      601 tatcaagcgg agcccttccc tgattttcta aaaagcatca ttgacaaagg cggtctgctc
      661 gcttatgtgg aggagcagtt gactctgagc aaaacgggag aaaatcaatg agcgattttg
      721 agctgaccga cgaacaacgc gaaatgcgcg agatggcgca caaatttgcg gaaaaggaga
      781 ttcgccccat cgccgcagaa tatgacgagc gagaagaagt gccttgggag gttattcaga
      841 aagcgaccaa ggccggcttc atgtcctact ttatcccaga gcgttatggc ggagggggga
      901 ttacggattt gttcacccac tgtctggtgg atgaggagat cttttggggc tgcgcgggga
      961 ttgccactat cctgggaggc accagtctgt gtgccacacc cattctcctc gctggcactg
     1021 aagagcaaaa ggaaaagtat ctatctcgtt tttgcgatct tgaccagcca aaacttggag
     1081 catttgccct gaccgagcca tccgcaggct ctgatccagc cagcatgatc acgcaagctc
     1141 accgggacgg caaatattac gtcctgaacg gctataaaac cttcatcacc aatgctggga
     1201 tcgctgatgt ctatgtcatt ttcgccaccg tggatgtcag ccgtggcgtg cgaggtgtaa
     1261 ccgccttcat cgttgacaaa gactgggaag gggtagtgcc agggaaaaaa gagaagaaga
     1321 tgggcatccg cgcttcccac acagccaccc tggcgttgga aggtgtgcgc gtgccggtcg
     1381 aaaaccgtct gggagaggag ggtgaggggt ttgctattgc tatgcggacc ttcgaacata
     1441 cccgcaccca catcgcaatc ggggcagtgg ggcttgcacg agccgctttt gaatatgccc
     1501 tgcagtatgc tcaggaaagg gtgcagttcg gcaaaccgat cagccaccat caagcggtgt
     1561 cattcatgct agccgaaatg gcaacccaaa tcgacgccgc ccggctgctc gcctggcggg
     1621 cagcctatct ggcagatcag ggcgtgccgt gcaccaagga agccagcatg gcgaaggcct
     1681 tcgccgcaga tatggcaatg caggcaacca ccgatgcagt ccaaatcctg ggtgggtacg
     1741 ggtacatgcg cgagtatccg gtcgagaaat ggatgcgtga tgccaagatc atgcaaatct
     1801 atgagggcac agcgcagatt caacgcatga tcatcgctcg ttttataacc aactaaatga
     1861 ataatggaga ggacatgaaa acatacaaca taggcgtaat agcaggtgat ggcatcggcc
     1921 cggaagtggc ggcagaagga ctcaaagtac tccgcacggt tagcgcccaa gagggctttg
     1981 agtgcaattt ggtggattat ccatattcgg gtacttatta cctgaagacg aaggagttag
     2041 tccctgagcg cgtcatcgac gagtggcgga cactcgatgc ggtctatctg ggtgccattg
     2101 ggcacccgga tgtggagccc ggcctggtag aacgctcggt aatcttaggc ctgcgctttg
     2161 gcctggacct ctacattaac ctgcgcccca tcaagctcta cgccgatcat ttgtgcccct
     2221 tgaaggataa aggacccaaa gatatcgact ttgtggtcgt gcgagagaat accgaaggct
     2281 tatactccca aatcggcgga ttcttgaaaa aaggcacccc tgacgaggtg gcgacggtca
     2341 cgggagtgta cactcgcaag ggcgttgaac gggcgatacg ttatgccttc gagctcgccc
     2401 ggcaacaggg gaaggcacgc gcagatcaga ggcagccgcg cctgaccttg gtggataaat
     2461 ccaacgccat ccggccgcag gatttatggg tgcgtacgtt cgaagaagtc gggcaggaat
     2521 atccggatgt ggaacaggat catgcctacg tggacgcctg caccatgtgg atggtgaaaa
     2581 accccgagtg gtttgacgtc atcgtaacta ccaacctgtt tggagacatc atcacggacc
     2641 tgggcgcaat gctgcagggc ggcatgggta tcgccgcttc aggtaatatc catcccggca
     2701 aaacttccat gttcgagccg atccacggct ctgcccccaa gtacactggc aagaacatcg
     2761 cttgcccttt aggtgctatt atggcagtga gcatgatgtt ggatttcctc ggggaaaaga
     2821 aggctgcgct aagcgtggag tcggctgtgg cccatctcct tgccagcgga gctattcctt
     2881 cggccgatgc acgcagcgga ttgagcacaa cccagattgg cgatatggtg gtcgaatcgc
     2941 tgcagaaagc ctaaccaggg aaagtgtcgg cctataccag gcttgttgaa aacccgatct
     3001 gatggtagga taactacctg gtttgacatc cagctcatta tccctagacc aacaaaagcc
     3061 atgaagaacc atgttcaata aagtcctcat cgcgaaccgg ggcgaaattg ctgtacgcat
     3121 catccgcgct tgccgggagc taggtatgcg aacagtggca gtgtattccg aggcggatcg
     3181 ccaggggcta catgtgcgct atgccgacga ggcatatcta ctgggaccgg ccccctcgcg
     3241 cgagtcttat cttcgcagcg ataagatcat tgagattgcg cgcaaatgcg gcgcaaaagc
     3301 aatccacccc gggtatggct ttttagccga gcgagccgac tttgcccaag actgccttga
     3361 cgtcgggttg gcattcatcg gcccgaagcc ttcatctatc gctgccatgg gcgataaagc
     3421 cgtggcacgt gctaccgtgg caaaggctgg cgtggctgtt gtaccaggca ccgaaggcga
     3481 aggcgcgttg ggcgatgagg agctgctgtc tgtggctcca gaaatcggtt tccccttgct
     3541 aatcaaagcc acggcgggcg gtggcggcaa aggcatgcgc gtggtgcatc atctagagga
     3601 aatgcactca ctattgcact ccgcacggcg ggaggcagag gccagctttg gggatggcaa
     3661 tgtttacttg gaaaagctgg ttgaaggagc gcgacacatc gaatttcaga tcctggcgga
     3721 tacccacggc aatgtgattc acctgggaga acgcgagtgt tccctgcagc gccgccacca
     3781 gaaactattg gaggaatcgc cctccccctt catcagtgat gatgacgaga tgcgccagcg
     3841 catgggcaat gtggcggtgc gcgctgcgca ggcagtaggc tatgtcaacg ccggaaccat
     3901 cgaattcctg gtagataagg ataaaaattt ttacttcctg gagatgaaca cccgcctgca
     3961 agtggagcat ccggtcactg aaatggtcac tggtgtggac attgtcaagg aacaaatccg
     4021 catcgctcgc gggcggcaaa tgcgctattc ccaggagaat atcaatctca acggttgggc
     4081 tatcgagtgt cgcatcaatg cagaggatgc atataataac ttcctgccat ccaccggtca
     4141 gatttctcac agcctgctgc ccaccgggcc gggtgtgcgc gtggataccg gggtctatcc
     4201 cggatttgaa atatcaccct attatgactc gctgatctca aaattgattg tatggggaga
     4261 aacgcgcgcc gaggcgatat tacgcatgcg ccgtgccctg gaagagtaca agatcgtcgg
     4321 cgtgcgcacc aatattccct tccaccaaaa tctaatggat tcgcatcgct tcatgggcgg
     4381 gcaattcgac acgcgttttg ttgaagagcg cttttctatg gaagcagccg aggagggcaa
     4441 atcccctcgc ccggagatcg ctgccatcat cgccactctg gtagcccacg agcaaaccca
     4501 acgcgccgcc cacattatcc agcgcaatga gcgggatacc agcaactgga aatggatcag
     4561 ccgctgggaa cgtatgcaga gatgagtcag gtgtgaggag tatggggtat ttatgaaata
     4621 cgtgaccacg atcggggaac gcgagtacat catcgaaatc gtcgacgagc gccatatcat
     4681 cctggatgga acccccttcg aggtggactt cgattcagtc agcgggcaac cggtttattc
     4741 cctgctgctg gatggacaat cgtatgagtc cttcgtctac tccagtgaag atggctggga
     4801 agtattgctg catggcacgc tttacccagc ttctgtggaa gatgaacgtg agaagcgctt
     4861 gcgcgccgcc gcaggaggca acatcgccga ggccggagaa ttctacttgc gggcacctat
     4921 gccaggcttg gtgatctcca tattggtcga tgagggtcag agcgtggaaa agggtgacgt
     4981 gctggtcatt ctagaatcga tgaagatgca gaacgaactc aagtcccccc gcccaggcac
     5041 gattgcgcgc atgcgcgtaa aagcgggaga tagtgtggaa caaaagcaga ccctgttgag
     5101 cgtcgtctga aaaccagaaa cgaaatgtaa ctcaccccaa tcccttttgg gcagaaccct
     5161 gcgttgcctg gaagtcgggg caaaactttc ccgcagtctc gggaaggatg caaaccaaac
     5221 ggcggggatg acgggcaggg accaaagatg aaaaaccagg aattggaagt aaagttcttt
     5281 gtcgccgatc tattggagat cgaggcgcgc ttgcgcgcct taagtgcgca cctcgagcaa
     5341 tcgcgcgtgc acgaggtcaa tttgcgattc gatacacccg atggcgatct ggggcgcggg
     5401 taccgcgtgc tgcgcttgag gcaggatgat gccgccaggc tgacctataa aggtcccagc
     5461 cagtaccagg agggtgtgcg cgcccgccag gagatcgagt tcaccgtcag cgacttcgag
     5521 gctgcacgcg ccttcctgga ggcgctgggt tatgttgtgt ccatgatgta cgagaagtac
     5581 cgcaccacat acgaactgga cggtgtgcat atcaccctgg atgagatgcc ttacggcgat
     5641 ttcatcgaga tcgagggacc agatccaaaa agtatacgaa gcatccaaga ccgcctcgag
     5701 ctagattggg agaagcgcgt ccctgagagc tacacggcgc tctttgagca actgcgcctc
     5761 gccctgagac tgcccttcaa agacctgagc ttcgccaact tcgacggact tgatatctcg
     5821 atgagtgtta tcagaataca acctgcagac ctgaacgaac aaagataagc acgtagaccc
     5881 atacaacaca actcaggata tatttcatgg ccaaagattt cagcctcaat gtcactcccc
     5941 caaacaagtt acagcgagcc gaatacgcct gcgatgacga ttggatccgc aacttcttcc
     6001 tgcaagcgga agttgggcac gtcgccacaa gctgggatga gcagcctttt atcacgccta
     6061 tcctattctg gtatgacccg caacaccacg agatttactt ccatacaaat atcaccgggc
     6121 gactgcgcgc caacattgag caccaggata aagtttgcat tgaagccagc cgcataggcc
     6181 gtgcgctgcc cgccaacacg gcactggaat ttgccttgca gtacgagagt gctgtcgcct
     6241 ttgggcgtgg gcgcgtgatt tcagatgacg acgaaaagct gcgcaccctg tatgggttga
     6301 tagaaaaata cttcccaaaa ctgaccccgg gagtgcacga ccgacccatc acagaccagg
     6361 aattgaagcg gacggcagtt tatgccatcg ccatcgagag ctggagcggc aagcgcaact
     6421 ggccggcgcg agccaaacaa ctccctgact ggcccccgct tggggatgaa tggttcgagg
     6481 aggacaattg aacgaggccg tcgtcctgag cgaaccaagc tcccgcctgg cacggggcta
     6541 cctgatctgc ctgacagcca ccgtaatctg gtcgaccacc ggcatattca tcggctacat
     6601 cacgcagaac ttcaatatgc cgccgcttct gctggcattt tggcgcgacc tgattgtaag
     6661 cgccacttta ttgactacgt tcaccctcct cagccgttct atgctgcgca tatcgaaaaa
     6721 caacctggcg tttctagcgt ggtatggact ggcgcttacc ttcctgaata cttcttggac
     6781 catctctgtg gctctgaatg gcgcggcggt ttccaccgtg ctggtctata gctccccggc
     6841 aattacagcc ctggtagggt ggcgtttgta cggcgagagc ctggatatgg ccaaaatatc
     6901 cgccattgtt ctgagtattt taggggctgc cctagtctca ggcgcatacc agccgcaggt
     6961 ctggcaggtc aacctgatcg gtgtggtagc cgggttgctc tcgggagtgg ctatggcaat
     7021 gtatagcttg atgggcaaag gggcttccct gcgccagatc aacccctggt cgaccctttc
     7081 gtacaccttc gccagtgcca cactttttct gttgatcatt aatttaactt cattgggaca
     7141 agcgcaaggc ttgtcccact caaacctgct ctggttgggg aatactccca ccccgtggat
     7201 tgtgctccta atcctggcaa tcggccccac gattggcggc tacggtctat atatggtcag
     7261 cttgggatac ctgcaagcca gcgtagctaa cctgatcgcc acgctggagc cggttttcac
     7321 agcgatcttg gcttacctgt tgctggcaga acgcctgaat agcgttcaga ttctgggcgg
     7381 aatcttgatt ttcacgggag tggtgctgct gcgcatgtat gaaggaagaa ggaaatgaca
     7441 gagttacttt tgggtccagt catcggtggc cttacacatg attccgctta cctgtggggg
     7501 cgggcggatg ggcccagcgt tttacacgcc tggttgggtc gcaatcccga cctgagcgac
     7561 gcccgtcttg cagggacttc tctccccctg tcatccgata cgggattcgc cggggtggcg
     7621 gtaactaaag agttacaacc gaattctcgc tactattacg ccctcaccct gagtggctcg
     7681 ccgcccactc ccgcaacgga gggggtttca tcttttacta ccgccccatc tcccggagag
     7741 cggctctctt tctccttcgc gttcggctct tgctttcgtc cagaaaacgc ggatggcggg
     7801 cgcatctttg acgccatcga ggagcaaaga aaggctgatg atctgcgctt tatcctgctc
     7861 ctgggcgacc agatctatgc tgacgcctac cagaacaatg gcatcggcaa gatcgcctgt
     7921 acgctgcaag aataccggga tgtgtacgct tacacctggt cgcggccgcc cttgcagagg
     7981 ttgctggcaa acctgccagc ttatatgacg ctggacgatc acgaggtgga cgacgactgg
     8041 cgctgggtag acagcaaccg ccgctgggca aacatcccgt ggtgggacaa ggtctcgcgc
     8101 tggctgaagc ggcgcccgct gcaagagcgt cgcttgccgg tgaagcgcgc ccaggacgcg
     8161 cttcaagctt attgggaaca ccaggggatg cacgcaccaa gctataagct tactccccag
     8221 ctcaaccaag ctggccaata cgagctggat gtcaaagacc ccggctcgct agcctacacc
     8281 ttcaccttcg gcgcggcggc gttctttgtg ctggacacgc gctcgatgcg cgttcgcaac
     8341 tcccgtgaga agatcatgct gggcgaggga cagtggcagg cgctgaaaac ctggctgcgg
     8401 gcggttaagg atgattatcc gatcaagttc ctggtcactt cctgttcgat gctttacccc
     8461 atgtggcatg acctgcctcg tgaccgatgg accggctacc cagaagagcg ggaccgcttg
     8521 ctgcactttc ttgcagccaa cgggatagat ggtgtctacc tgctagcagg agatttgcac
     8581 tcatctcatg cggtgtatgc cgaactctat ggccccgagg ggcgttcttt acccttgtgg
     8641 gagttctgtt ccagcccctt cgaacaagat tgcaatcgct gggggcgcta tacctacaag
     8701 ccactacata ccagccctgt gaaagtgagc tatcgcgcct tcagcgtaca tcaggtcaat
     8761 tatggcgtgg taaaggtcga tttttctcgc aaagatcgcc caaacgtcac tttccagatt
     8821 catgaccagc aaggtgaaaa gctcgaggct atttcggtgt aattttagag aaggtctcca
     8881 tctttccccc actccaagag atcgacaagc ccctcttctc caaccaacct gggcaataga
     8941 gcttctaact gctctgggtt acccgtggtg agataaatca cttcgccctc ttcccgatcc
     9001 ggatgaggca acccgcgcgc ctccagcacc ctccccacct gccgcgccac tgccggggcg
     9061 ggatccacca cgcgcacact ggtaccgacg atttcttgga tcagcgggat gacaaaggga
     9121 taatgcgtgc agcccagcac taccgtatcg atcccccggg cgagcatggg gtgcaaggca
     9181 ttttccagga tgcggcgcgt ttcagtgcta tccagattgc ctgcttcgat ctgcgctacc
     9241 aatcctgggc aggtatgctg caacaagatc acatcctggg caaaacgctc cacgaccgaa
     9301 gcatataggg ctccttgaaa ggtcgccggg gttgccaaca cgcccaccac accgctctgg
     9361 gtaccctcgg cggctggctt gactgccggt tccatgccta cgaagggcac ctgcgggaag
     9421 gtctgacgta aatattgcaa tgccgccgcc gaagcggcgt tgcaagctac aacgaccagc
     9481 ttagcg
//
LOCUS       DBNHOH010000713         1645 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1261656, whole genome shotgun sequence.
ACCESSION   DBNHOH010000713 DBNHOH010000000
VERSION     DBNHOH010000713.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1261656"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..892
                     /locus_tag="AC16V7_12435"
     CDS             35..892
                     /locus_tag="AC16V7_12435"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000098 - sulfur amino acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HIR6257973.1"
                     /translation="MSYQTLVSAEVLMSHLGDPNWVIVDCRFTLTDTERGRQDYRQAH
                     IPGAVYAHLDEDLSTPHIPGSTGRHPLPAVDSLVATFSNWGISQGMQVVAYDDDPSAS
                     GAIAARLWWCLRWLGHDTVAVLDGGWLRWLKLGHPTQGGEERRKSRRFIPSLRLELIA
                     SAQEVDQIRSDSAYRLVDSRSADRYRGENETIDPVAGHIPGARSVPFVENVGKDGLFR
                     PKDELKARFNSLLGEVPGQHVIFYCGSGVTAAHNLVAFQHAGLGEARLYAGSWSEWIV
                     DLSRQVARG"
     gene            complement(894..>1645)
                     /locus_tag="AC16V7_12440"
     CDS             complement(894..>1645)
                     /locus_tag="AC16V7_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257974.1"
                     /translation="CLILMVFGMAFIIPARAQDEVELTLNLTRDFGYSSGTGRIQGRF
                     TMKVNGPANLERVVFLIDDLMVGEDTQAPFLLQFSTGDYALGVHTMSAVGYTSDGREL
                     RSNAQRREFVSAEEGWQSAMKIAIPIIGLAFAAMLFSFILPSLLGKRVSMATPLGAPR
                     SYGLLGGTVCPKCSRPFGMHVWGMNLVIGKLDRCPYCGRWSLVRRVPNELLKVAEAAE
                     LELAQGNAETPAHSAEEKLHRDLDDSRYLDL"
BASE COUNT          381 a          426 c          464 g          374 t
ORIGIN      
        1 caagaattag gctatttaga aacaggattt tcagatgagt tatcaaacac tagtttcagc
       61 tgaagtattg atgtcgcatt taggcgatcc gaattgggta attgtcgact gccgcttcac
      121 gctgaccgat accgagcgag gccggcagga ttaccggcag gctcatatcc ctggggcggt
      181 gtatgcccat ctggatgagg atttaagcac gccgcacatc cccggctcaa cagggcggca
      241 tccattgcca gcagtcgatt cgctggttgc cacattttcc aactggggaa ttagccaagg
      301 gatgcaagta gtagcttacg acgatgaccc atctgccagc ggggccattg ctgcccgatt
      361 atggtggtgc ttgcgctggc taggtcatga taccgtcgct gtgctggatg gcggttggct
      421 gcgctggctg aaattgggcc atccgacgca gggtggggag gaaaggcgca agtccaggcg
      481 atttattccc agcctacgcc tagaattgat agcaagtgcg caagaggtgg atcaaatacg
      541 ttctgattca gcctatcgat tggttgactc tcgaagcgct gatcgctacc gcggcgagaa
      601 tgagaccatc gacccggttg ccggacatat tccgggggcg aggagtgttc cctttgttga
      661 gaatgtcggt aaagatggat tatttcgccc gaaggatgag ctcaaagctc gctttaactc
      721 cctgctgggt gaagtgcccg ggcaacatgt tattttttat tgcgggtcgg gcgtgactgc
      781 agcccataat ttggtcgctt ttcagcacgc tggcctaggc gaggcgcgcc tttacgctgg
      841 ttcgtggagc gagtggatcg tagatcttag caggcaggtg gcgagagggt gattcagaga
      901 tccaaatagc gcgagtcgtc caggtccctg tgcagttttt cttccgcaga atgggcgggg
      961 gtctcggcat ttccctgagc caactccagt tcggctgcct ccgcaacttt gagtaactca
     1021 ttaggcacgc ggcgcaccag gctccatcta ccgcagtaag ggcagcggtc taatttgccg
     1081 atcaccagat tcatgcccca tacatgcatc ccaaatgggc ggctacactt tgggcagacc
     1141 gtgccgccca acaaaccgta agaacgaggc gcacccaagg gtgttgccat gctgacacgc
     1201 ttccctaaga gactcggcag gataaaagaa aacagcatgg cggcaaatgc aaggccaatg
     1261 atgggaatag cgattttcat ggcagactgc cagccttctt ctgcgctgac gaattcacgc
     1321 cgttgggcat tagatcgcag ctcgcgccca tcgctggtgt atcccactgc gctcatggtg
     1381 tgaacaccca gcgcatagtc gccagtggaa aattgaagca agaatggcgc ttgggtgtct
     1441 tcgccaacca tcagatcatc gataaggaat actactctct caagatttgc aggcccgttt
     1501 accttcatgg taaacctgcc ctggatgcga ccagtcccag acgagtaacc gaagtcgcgg
     1561 gtcaagttca gggtaagctc aacttcatcc tgggccctgg cagggatgat aaaagccatc
     1621 ccaaatacca tcaatattaa acata
//
LOCUS       DBNHOH010000714         4121 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_43781, whole genome shotgun sequence.
ACCESSION   DBNHOH010000714 DBNHOH010000000
VERSION     DBNHOH010000714.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_43781"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..899)
                     /locus_tag="AC16V7_12445"
     CDS             complement(75..899)
                     /locus_tag="AC16V7_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257975.1"
                     /translation="MSEPIIGVGLTGELSLRVSVATLSRVVFPHPEDGVPMLALEHKA
                     TLAAGEADRVDVKAQPFGGSIRILKPNRLLTLLGSFNFDSQRSRSEQDFRVYIRPSSW
                     EEVREFCLQNMGQEAGPDLEIDPSRELVEEFEDALGIQLKLNQYVVKPFGIVVENEPA
                     PTTNVRAAGSPTARIYRVYEVQIQDSAVWRLMVTNSQTHPKQILQRLALDDSQKGGRG
                     RATAMLVAAEEEIRAAYSALLPEMLGMPLAFAGTLLDGNVPAILKGISAPKYRYLR"
     gene            complement(905..>4121)
                     /locus_tag="AC16V7_12450"
     CDS             complement(905..>4121)
                     /locus_tag="AC16V7_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6257976.1"
                     /translation="KVQQISPSEFTNLHIPEMAYDQVEAENALAIFYTIAGQSLHNFL
                     TLSSHQRQSHIESLFSATNHILLDSWNAGLTFEQIDHPRDLLQKWLGFRLDAGQNIEI
                     FLSEVCQLPPNIPGFIVQGNILPNPLYYARNIDAWTSVRAGDAMVGLQHSDLNTNNIL
                     AKFSRQGDELEGYYLIDFALFKENMPLLYDQRYLEMSYLTHTISQGIYNSVVGLICRY
                     GEQDILEPDQTAIEMTGVNAAIRAGRIAFGDWVRENHPSLHDDLWGQYWLAGTAAGLS
                     YCHKAGQADGIRLAGLIYASANLKQYFKLFGLPMPTGASQLYAESQIGGMPAAGFTLA
                     SPARKPNHNLPAPPTNFIGRTAELAELTDLLLRPEVRLVSLTGPGGTGKTRLSLEAGR
                     ALLDRFPQGVYFVDLAQINDPSLVSATTAHTIGIREGGGRPPLETLKDYLAGRGMLLI
                     FDNFEQVVGAAADISQLLAAAPGIKALVTSRTPLHLRGEHEYPVSPLDMPPDAYQSID
                     QAIKYEAVALFRQQARTVQPAFDLTEDNRAAVIEICRRLDGLPLAIEIAAARIKMLSP
                     QALLDRLDHSLSLLVGGAKDLPNRQQTLRGTIDWSYELLQPVEQILFTRLGIFAGGFT
                     LNAADEICKPMGEVDVFSGVETLLNNSLLRQVHSASSEPRFDMLQTIREYALEKAAEA
                     GIMTELQWAHCGYYSRMAAAAGNLGEGSFGAHSVIWLQRYEEEYDNFRVALSWGIKHP
                     EEGFPLVIAMMNQLAWFWYRYGYLQEGSEWAERTLAATQGMGDSPARAWALVGRAYLA
                     LWSGDLLIAARYGREAVEMCQRVSFDLGLSLAKLTFGTTLINQGKDREAYPHLVDAVE
                     LYDQQDQPWMKGTALVHLANVSLGLGDPEQATQWLDMAMPFLLNSGDVWAMAFGLNNY
                     GEVARAQGDYDRAEDFYRRTDELYKQADAKGDQARLLHTFGYIAQHKGDLNGARSLFL
                     ESLNDFRKLGNHRGIAECLAGLAGLGAIQGKHEWATPLLSSAESQLKAIGGAWWPADR
                     VEIERARERLRAALDDRFAELWAQGQSMGVEEAIAYATNEA"
BASE COUNT          902 a         1207 c         1080 g          932 t
ORIGIN      
        1 aagccaatgt agcctcaccg cacaccatcc gcgatgataa tgccggtctc ttccgccacc
       61 ccaaccgacc cccgctagcg aagatatcga tatttcggtg cagaaatacc cttcaatatt
      121 gcaggcacgt tcccatccag gagcgtgcct gcgaatgcca gcggcatgcc cagcatctcc
      181 gggagtagag ccgaataggc ggcgcgaatc tcctcctccg cagccaccag catggcggtc
      241 gccctgcctc gcccgccttt ttgagaatcg tctaacgcta atctctgcaa gatctgtttg
      301 gggtgtgtct ggctgttggt caccatcaat cgccatacgg ctgaatcttg gatctgcact
      361 tcgtaaaccc ggtagatccg ggcggttggg ctgccagcag cacgcacgtt ggtcgtgggg
      421 gccggttcat tttccaccac gatgccgaac ggcttcacaa cgtattggtt taacttgagc
      481 tggatcccca gcgcatcctc aaactcttcc accaattccc tggaagggtc tatttccagg
      541 tctggacccg cttcctgacc catattctga aggcagaatt cgcgcacttc ttcccagctc
      601 gaggggcgga tatagacccg gaaatcttgc tcggaacgcg atcgttggct gtcgaagttg
      661 aagctgccca ggagtgtcaa aagccggttg ggctttaata ttcggataga ccccccaaac
      721 ggctgggctt tcacatctac cctgtcagcc tctccagcag ccagggtagc tttatgctcc
      781 agcgcgagca tcgggactcc gtcctctgga tgggggaaga cgacccgcga aagcgtggcc
      841 acgctgaccc gcaagctcag ttcacctgtg aggccgacgc cgatgattgg ctcactcaca
      901 gcgattacgc ctcattcgtg gcgtaggcaa tcgcctcttc aacacccata gattggcctt
      961 gcgcccagag ctcagcgaag cggtcgtcga gagcggctcg cagacgctct ctcgcccgct
     1021 caatttctac ccggtctgca ggccaccagg cgccgccaat cgccttaagc tggctctccg
     1081 cgctgctcaa caaaggtgtg gcccactcat gttttccttg tatggctccc agaccagcca
     1141 ggccagccag gcattctgcg ataccgcgat ggttgcccaa ttttcgaaaa tcattgaggc
     1201 tctccaagaa taacgagcgt gccccattaa gatcaccctt atgctgggca atgtagccga
     1261 atgtgtgtag aagacgtgcc tggtcgcctt ttgcatccgc ctgcttgtac aattcgtcgg
     1321 ttcgccggta gaaatcttct gctctatcat agtccccctg ggctcgagca acttctccgt
     1381 agttattcaa tccgaaagcc attgcccata catcaccaga attcaacaag aatggcatgg
     1441 ccatatccaa ccattgggtg gcctgctctg gatcgcctaa tcctagggac acattggcta
     1501 ggtggaccaa ggctgttcct ttcatccagg gttgatcctg ctggtcatag agctcgaccg
     1561 catccaccag gtggggatag gcttctctgt ccttgccttg attgatcaat gtcgtcccga
     1621 aggttagctt agccagggat aagccaaggt caaagcttac ccgttggcac atttccacag
     1681 cttcccgccc atatcgcgcg gcaatcaaca ggtcgcctga ccacaaagcc agatatgcgc
     1741 gcccgactag cgcccaggcg cgcgccggcg aatcgcccat cccttgtgta gccgccaggg
     1801 tgcgttcggc ccattcactg ccctcttgca agtaaccata gcggtaccag aaccaggcca
     1861 gctgattcat catcgcaatc accagtggga agccttcttc aggatgcttt ataccccagg
     1921 agagggccac acggaaatta tcatattctt cttcatagcg ctgcagccaa atcacagaat
     1981 gggcgccaaa actcccttcg cccaaatttc ctgcagccgc cgccattcgt gaataatagc
     2041 cacagtgagc ccattgaagc tcggtcatta tgccggcttc tgctgctttt tccaatgcgt
     2101 actcgcgaat cgtctgaagc atgtcgaagc gtggctcact gctggccgaa tggacctggc
     2161 gaagcaagct gttatttagt agcgtctcaa caccagaaaa aacgtccact tcgcccatcg
     2221 gtttgcagat ttcatctgca gcattcaggg tgaacccccc ggcaaagatt cccaatcgtg
     2281 tgaagaggat ctgttccaca ggttggagca attcataact ccagtcgatg gtcccgcgca
     2341 aggtttgctg ccggttgggt aaatccttgg ctcctcccac cagcaggctc aagctgtggt
     2401 ccaggcgatc aagtaatgcc tggggggaga gcatcttgat gcgggcggcg gcgatctcga
     2461 tcgccagggg aagaccatca aggcggcggc agatctctat gaccgcagca cggttgtcct
     2521 ctgtaagatc gaatgctggc tggacagtcc tggcttgctg ccgaaaaaga gccaccgctt
     2581 catatttaat agcctggtca atagattggt atgcatccgg gggcatgtcc agaggggaga
     2641 ctgggtattc atgctccccg cgcaggtgca aaggggttcg actggtgacc aacgctttga
     2701 tcccaggagc agcggcgagc agctgcgaga tgtccgcggc agctccaacg acttgttcga
     2761 aattatcgaa aatcaggagc attcctctgc cagccaggta gtctttcaaa gtctctaatg
     2821 gcggtcgacc tccgccttca cggatcccga tcgtgtgagc ggtggttgcc gaaaccagtg
     2881 acggatcgtt gatttgcgcc aggtcaacga aatacacacc ttggggaaag cggtccagca
     2941 aagctctgcc tgcttccagg ctcaggcggg tttttcccgt cccacccggc cctgtcaacg
     3001 aaaccagccg cacctccgga cgaagcagca aatcggttaa ctcagccagt tcagccgtcc
     3061 ggccgatgaa gttcgtcggc ggtgctggca aattatggtt cggttttcta gccggggaag
     3121 ccagcgtgaa gcctgctgcg ggcatgcccc cgatctgact ttcggcgtac aactggctgg
     3181 caccggtggg catgggaaga ccaaatagct taaaatactg cttcaggttg gcggaggcat
     3241 aaatcagccc cgccagtcgt attccatcgg cctgacccgc tttgtggcag tagctcaacc
     3301 ctgccgctgt tcctgcaagc cagtattgac cccacaagtc gtcatgcaag ctgggatggt
     3361 tttcccgtac ccagtctcca aaggcgatcc gccctgcacg tatggctgca ttgacaccag
     3421 tcatttcgat cgctgtttgg tcgggctcca ggatgtcctg ctccccatag cggcagatca
     3481 ggcctaccac gctgttatag atcccctgcg agatcgtatg ggtcagataa gacatctcca
     3541 ggtagcgttg atcatacagt agtggcatgt tctctttgaa gagggcaaaa tcgatcaggt
     3601 agtagccttc taattcatcc ccctgtcgcg aaaacttggc caggatattg ttcgtgttta
     3661 aatcgctgtg ctgcagcccg accatagcat ccccagccct gactgaggtc caggcatcga
     3721 tatttcgcgc atagtacagc ggatttggga ggatattacc ctggacgatg aaaccaggga
     3781 tattgggggg caactgacag acctcgctca agaaaatctc aatattctga cccgcatcca
     3841 gacgaaaccc cagccacttc tgcagcagat cccgggggtg atcaatctgc tcaaatgtca
     3901 gacctgcatt ccagctatcc agcaatatgt ggttggtcgc tgagaacaag gattcaatgt
     3961 ggctctggcg ctggtggctt gagagcgtga ggaaattgtg cagcgattgg ccagctatcg
     4021 tataaaaaat cgccagggca ttctcagctt caacctgatc gtaagccatt tcggggatgt
     4081 gcagattggt gaactcagag ggggaaatct gctgtacctt t
//
LOCUS       DBNHOH010000715         1993 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1261704, whole genome shotgun sequence.
ACCESSION   DBNHOH010000715 DBNHOH010000000
VERSION     DBNHOH010000715.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1261704"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..608
                     /locus_tag="AC16V7_12455"
     CDS             3..608
                     /locus_tag="AC16V7_12455"
                     /inference="COORDINATES: protein motif:HMM:NF025338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6257977.1"
                     /translation="MINTAIDLFNLQGTSNVSTNHIAAAMDISPGNLYYHFRNKEEII
                     LAIFEQMVAEMDQVWKFQPAFSLKNFVELAFQTIEGLARYHFFQRETIPLMQRDTVLK
                     TRYEEMQQHRLAQLEALLQHFVTSGLLVQFPNPQVMASLAKACWMIGDRWLVMLDMEG
                     KAPTRKNITEGILLILQLLEPYFSQQGKEEYSRLIQNLEEI"
     gene            605..1324
                     /locus_tag="AC16V7_12460"
     CDS             605..1324
                     /locus_tag="AC16V7_12460"
                     /inference="COORDINATES: protein motif:HMM:NF025687.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HIR6257978.1"
                     /translation="MKDSRIIKVFSVSSLILTILFVAVFTYLAINYDYPDNLDYPAQT
                     TLPSIDQAGYDWRAIWFFYALLPVGYILVGMTAYMALKPTNKSLIRMGVLFTVLASIF
                     GTLGLARWPTIMPPIAKAYQTADASQRAVLEAIFNGVNLYLGNYLGEFMSEFLFGAFI
                     VLVGITIFAAKGNGYPIWFGVISIISGVLIVTGSTRMFFELTKAANEYTTMVLPVYLI
                     LFSGLSYRAASMLRTISKTAG"
     gene            1501..>1993
                     /locus_tag="AC16V7_12465"
     CDS             1501..>1993
                     /locus_tag="AC16V7_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257979.1"
                     /translation="MAAPTSDQATIPPPEIIDTATPPAPTAIPSAAAITTNYQNHSLG
                     ISLDYPAVWHVQEEIPDQPFAVLIASFDPASPPHKLEWDSQTVSISLRLLPQENTPQD
                     LNSWAESAKQEAVADHLEVFSEESIELAQGIQAVRLTLVSGSGGIIDQLLTILDGRPY
                     EIIV"
BASE COUNT          528 a          502 c          444 g          519 t
ORIGIN      
        1 tgatcatcaa tacagctatc gacctgttca acctgcaagg cacctccaac gtctctacga
       61 accacatcgc tgcggcaatg gatatcagtc cgggtaatct ctattatcac tttcgcaaca
      121 aggaagagat cattctggca atctttgagc agatggtggc tgagatggat caggtttgga
      181 aatttcagcc agcattctcg ttgaaaaact tcgtggaact cgcatttcag actattgagg
      241 gattagctcg ctatcacttc ttccagcgtg aaactattcc tctgatgcaa cgcgacaccg
      301 ttctaaagac acgttacgaa gaaatgcagc aacacaggct ggctcagtta gaagctctcc
      361 tgcaacattt tgtcacctca ggtctgctag tacaatttcc caacccacag gtgatggcct
      421 cactggcgaa agcctgctgg atgattggtg accgatggct ggtcatgctg gacatggaag
      481 gcaaagcgcc tacccgcaaa aatattactg aaggaattct cctgattctg caactgctag
      541 aaccctactt ttcccaacaa gggaaagaag aatactcacg cttgatccaa aatttggagg
      601 agatatgaaa gacagtcgca tcatcaaagt attttcggta tccagtctga tactgacgat
      661 tttgttcgtg gcagtgttca cttacctggc gatcaattat gattatccgg ataatctgga
      721 ttaccctgcc caaactaccc tgccgagcat tgaccaagca ggttacgatt ggcgcgcaat
      781 ctggttcttc tacgccctgc tgccagttgg ttatatcttg gttgggatga ccgcctacat
      841 ggccctcaag ccaaccaaca agagtctcat tcgtatgggc gtgcttttca ctgtcctggc
      901 gtctatattt gggacactcg gtctagcgag atggcctacg attatgccgc cgattgcaaa
      961 agcctaccag acagcggacg ccagccaaag agcagtactg gaagcaatct tcaacggagt
     1021 taacctctac ctgggaaatt acttaggcga gtttatgagt gaattcttgt ttggcgcttt
     1081 catcgtcctg gtaggcatta caatatttgc tgcaaaggga aacggttatc ctatatggtt
     1141 tggtgtaatt tcaataattt ctggtgtctt aattgtgacg ggctctacgc gcatgttttt
     1201 cgagctgaca aaagctgcaa atgaatatac aacgatggta ttaccagtat acttaatttt
     1261 atttagcgga ctgagttatc gagcggcctc gatgctacgc actattagca aaacggctgg
     1321 ctaggcgaaa ctatcgaagg aacaatttga accttttaac cgtccataag gttgttatta
     1381 agctataaaa atcgtatctc tgctctgctt ggatttggtg aacaaatgat tctcaagaaa
     1441 tatcttatca caagcataat tactggtttg ctcatagctt cctgtaatgc ggcatctcga
     1501 ttggcagcac caacttccga ccaggcaacg ataccgcctc cagagattat tgatactgct
     1561 acacccccag caccaacagc tatcccttca gctgcagcga tcactaccaa ttaccaaaac
     1621 cattctctag gcatcagcct ggattatcct gcggtctggc acgtgcaaga agagatcccc
     1681 gatcagccat tcgctgtcct tatagcttcc ttcgaccctg cctctccccc gcacaagctg
     1741 gagtgggata gccagactgt gagcatcagc ctccgcctgc ttccgcagga gaatacacct
     1801 caagacctga atagctgggc agagagcgcc aaacaggaag cagttgcaga tcacctggag
     1861 gtcttttcag aggaaagtat cgaactcgcc cagggcatac aagccgtacg tttgacgctg
     1921 gtatcaggtt ccgggggaat aattgatcaa ttgcttacca tcctcgacgg acgcccctac
     1981 gaaatcattg tcc
//
LOCUS       DBNHOH010000716         1356 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1455101, whole genome shotgun sequence.
ACCESSION   DBNHOH010000716 DBNHOH010000000
VERSION     DBNHOH010000716.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1455101"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..545)
                     /locus_tag="AC16V7_12470"
     CDS             complement(228..545)
                     /locus_tag="AC16V7_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257980.1"
                     /translation="MIVRYWRTITLASGADRYHEYLDKNLIPKYRAVDGLEGLFVLRE
                     VRGGLAHFLLISLWKSHQAILSVMAPKAGDAHQPASVNHSYLAFETRAKNYEVLNMMA
                     RIK"
     gene            complement(844..>1356)
                     /locus_tag="AC16V7_12475"
     CDS             complement(844..>1356)
                     /locus_tag="AC16V7_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257981.1"
                     /translation="CIRLARRFWVVLLIFALAMLLQYLATGIAAAGFAILKPVSVIDE
                     YVRQNPESTAAKAREFAGLLGFHGLIGFHQAWTMSLSAIPLVLVSLLELFPWARRRPL
                     LAAPLYSVSMVLFSFLWFEQMPALRGYPISSLHLAQAALISALVGLVTGNLGLRLARL
                     VDTPNTSSTG"
BASE COUNT          360 a          347 c          359 g          290 t
ORIGIN      
        1 tgacacagga caatatgtcc caacttgttg agatcgtgga ttaaactgtc agaagcgatg
       61 cagttggaga agtgaagata gatttcggtg gagatattgt ggattctatt gacaaaccat
      121 ccgtctctta gagagtcgcg ctttcctgga tataccctta cccaagaatg aggttgcaaa
      181 taccgccgtg aataagctcc gtgtttttgg agattttatg gaactggcta cttgatcctc
      241 gccatcatgt tcagtacttc gtagtttttc gccctggtct cgaaagcgag ataagaatga
      301 tttacagaag cgggctgatg agcatcccct gctttagggg ccataacgct taagatagct
      361 tgatgggatt tccacaaaga aatcaacagg aaatgcgcca agccgcctcg aacttcccgc
      421 aagacgaata acccttccag accatcaacc gctcgatact ttgggatcag gttcttgtcc
      481 aggtattcat gatagcgatc agccccagat gctaatgtaa tcgtgcgcca atatcgtaca
      541 atcattctag cctcccctgg aaacaaatcg acgagcgttc cagcctagac actatgcatg
      601 atgcgatgct gtaatacttg ttacagttta aggcacaacg cttacgatga gggggaacta
      661 tttgccccgg aactgcccca aaaagtgata catttctcgc aacacatacc gcttgaatcc
      721 cgctctggta gctccgtagg taaccttccc cctaccccct attcggtggt gaagggagaa
      781 tcttcacggt taacggtagc gctacccctc gttcttggtt atgcagttaa ataagatgtg
      841 tacttaccca gttgaggagg tattaggggt gtcaaccaag cgcgccaggc gcaagcccag
      901 gttgccggtt accaacccga ccagggcgct gatcaacgcc gcctgggcca agtgcaggct
      961 gctgatgggg taaccgcgca aggcgggcat ctgctcgaac cacaggaagg agaacaatac
     1021 catgctcaca gagtagagcg gggcagccag cagtggtcgg cggcgcgccc acggaaaaag
     1081 ctccaacaag gagaccaata ccagcggtat ggcagataga gacatggtcc aggcctggtg
     1141 gaagccgatc aggccgtgaa aaccgagcag accggcgaac tcgcgggctt tggcggcagt
     1201 cgactctgga ttctggcgca catactcatc gatgactgag acgggcttta gtatcgcaaa
     1261 accagccgcg gcgatgccgg tagccaggta ttggagcagc atagcgagag caaagatcaa
     1321 gagcacaacc cagaaacggc gcgccaggcg gataca
//
LOCUS       DBNHOH010000717         2151 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_236528, whole genome shotgun sequence.
ACCESSION   DBNHOH010000717 DBNHOH010000000
VERSION     DBNHOH010000717.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_236528"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="AC16V7_12480"
     CDS             complement(<1..533)
                     /locus_tag="AC16V7_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6257982.1"
                     /translation="MVKSETFSYEAKDIQVLEGLEAVRRRPGMYVGGTDLKALHHLIY
                     EVVDNSIDEALAGACDRIEIIIHPDSSVTVSDNGRGIPVDIHPQIKKPALEVVMTMLH
                     AGGKFGGGSYKVSGGLHGVGVSAVNALSEWCQVEVRRDGKVHFQRYERGYPKNPVSVI
                     GKSDSKITGTTTTFKCD"
     gene            complement(657..1676)
                     /locus_tag="AC16V7_12485"
     CDS             complement(657..1676)
                     /locus_tag="AC16V7_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257983.1"
                     /translation="MSTTDNQALPTQPSLMTSLMAGFDAVTNHLELILFPIILDLFLW
                     FGPHLRLKELVVEFTNQIGNLSGLDTPEVSNALQLNRELWLLVADRLNMFSALRAYPV
                     GIPSLMASRQPLEAPLVQPVGWEVSSVEGAAVWWFILSVMGLVFGALFFVLVAQASLH
                     GKIHWQIAIGQWPRSSLQVILLTLFIGILMLAISIPGSCLITALTLTGLPVSQISLLL
                     FGAVALWVLFPFLFSAHGIFTYQHKMWTSVRQGARVVRMTLPKTSLLFLSIVVISEGL
                     DTLWRVPAENSWMSLIGVTGHAFVSTSLLAASFIYYRDATRWVQRLIQQSLMVESKKL
                     RERIT"
     gene            1805..>2151
                     /locus_tag="AC16V7_12490"
     CDS             1805..>2151
                     /locus_tag="AC16V7_12490"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR6257984.1"
                     /translation="MSIMTFELQQADIFYQLTPAQLEKISGIITEKIFNYGEMIFWEG
                     THSDELYVIAQGEVDILINPSLVSDQPEQEQEPATIATLRRGQSFGEIALIDRGIRSA
                     SARAAQANTRLLV"
BASE COUNT          596 a          556 c          484 g          515 t
ORIGIN      
        1 ctatcacatt taaacgtggt agtcgtacct gtgatttttg agtcggactt gccgatcacg
       61 ctcactgggt ttttcggata accgcgctcg taacgctgga aatgcacttt gccatcccgg
      121 cggacttcta cctggcacca ttccgataag gcattaaccg ccgatacgcc caccccatgc
      181 agaccaccgg agactttata gctacctccg ccaaacttgc cgccggcatg tagcatagtc
      241 atcactactt caagggctgg cttcttaatt tgggggtgga tatctaccgg gatgccacgc
      301 ccattatcgc tgaccgtaac actgctatcg ggatgaatga taatttctat acggtcacac
      361 gcccctgcca gagcttcatc gatggagtta tccactacct catatattag gtgatgtaaa
      421 gccttgaggt cggtgccgcc cacgtacatc ccaggtcggc gccgcacggc ttccagcccc
      481 tccagcactt gtatatcttt tgcctcatac gagaaagttt cactttttac cacgtcatct
      541 cctcaaacga tatcagagtt gtaagattag agaatcagga aaaaatgggc gaatattgag
      601 acgtattgat acgcccataa attttatttt tcgattttct tgtcttaatc ctagagtcac
      661 gtgatgcgct cacgcaattt cttgctttca accattaatg actgctgaat taatctttgt
      721 acccatcgtg tagcgtcgcg atagtaaata aagcttgctg ccagcaagct cgtagacaca
      781 aacgcatgcc cggtaacccc gatcaacgac atccacgagt tttctgcagg tacccgccag
      841 agcgtatcca gtccttcgct gatcaccaca atactaagga aaagaaggct ggttttggga
      901 agcgtcatac gaactactct ggctccctgc cggaccgatg tccacatttt gtgttggtag
      961 gtgaagatgc cgtgagccga aaacaagaag ggaaatagca cccaaagtgc cacagcacca
     1021 aaaagcaaca ggctgatttg ggaaaccggt aagcccgtca atgtcaacgc ggtaatcaaa
     1081 cagctccctg ggatgctaat cgctagcatc aatatcccaa tgaacagggt tagtaaaata
     1141 acttgcaaag aggaccgagg ccattgacca atcgctatct gccagtgtat cttgccgtga
     1201 agagaagcct gcgccaccaa aacaaagaat aaagcaccaa acaccaaacc cattacactg
     1261 agaataaacc accagactgc cgcaccctca accgaagaca cttcccaccc cactggttga
     1321 acgagtggcg cttccagcgg ttgacgagaa gccatcaggc tgggtattcc aactggataa
     1381 gcccgtagtg cggaaaacat gttgagccta tcagcaacca atagccacag ctcgcggttt
     1441 agctgcaatg cattagatac ctcaggtgta tcgagcccgg ataaattccc tatctggttt
     1501 gtgaactcta cgactagctc cttcaggcgt aagtggggtc cgaaccaaag gaataaatcc
     1561 aaaatgatcg ggaacagaat caattcaagg tgatttgtga ccgcgtcaaa accagccatc
     1621 agggaggtca tcaggctggg ttgggtaggc aaagcctggt tatcagttgt gctcatttcg
     1681 accccatttt accatagggg gtcttaaaga acaccgcgct agaattgaca tttttgtgat
     1741 ttatgctaga ctttatagac tttagcacct cgataaaata tcaccagaga tctataaaaa
     1801 tcgtatgtcc atcatgacct tcgagttaca acaggcagat attttctacc aattgacccc
     1861 agctcaattg gagaagatct cgggcatcat cactgaaaaa atatttaact acggtgagat
     1921 gatcttttgg gaagggaccc acagcgatga attatatgtc attgcccagg gagaagtgga
     1981 tattttgatc aacccttcct tggtgagcga tcaacctgag caagaacagg aacccgcgac
     2041 gattgccacc ctgcgtaggg ggcaatcctt cggtgaaatt gcgttgatcg atcgaggcat
     2101 acgctcggca tcagcccgcg ccgcgcaagc taacacacgc ttgctggtaa t
//
LOCUS       DBNHOH010000718         4252 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1518670, whole genome shotgun sequence.
ACCESSION   DBNHOH010000718 DBNHOH010000000
VERSION     DBNHOH010000718.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1518670"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="AC16V7_12495"
     CDS             complement(<1..432)
                     /locus_tag="AC16V7_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257985.1"
                     /translation="MTYQGGSTLPPDLLERLAIEGFESKTVKTCLRRLRLRFSNLAKV
                     ASIVRVKAPDCPTAETYLANCPVFDVYLHRALGQAGEHGAGRRVGNAPVAGASVLISL
                     GIVDVGFGDYAAARTRIEHGLSIAREIDHPLGMADALTNLGC"
     gene            complement(552..716)
                     /locus_tag="AC16V7_12500"
     CDS             complement(552..716)
                     /locus_tag="AC16V7_12500"
                     /inference="COORDINATES: protein motif:HMM:NF016811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HIR6257986.1"
                     /translation="MVRCPVCGMMVNENTAPSSEYKGKKYYFMNPTHKALFDKDPEKF
                     LSRQLNRSDH"
     gene            complement(1209..2627)
                     /locus_tag="AC16V7_12505"
     CDS             complement(1209..2627)
                     /locus_tag="AC16V7_12505"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6257987.1"
                     /translation="MMKFTVSEIFQQSRPPDATSPARSTKRLSLARLAPSRGLSLLLF
                     SSAISEVACNIVYIVLLERAYLLGRGSSSIGILMIIQSTAPVFFGSWMGGLADQLRFK
                     RSAVIANSAIAALVFSLTFAKIVTFVYIIAFLIMLGRLLLIPARLGLVAQLTDRARLM
                     EANTTLSILSGVGSFIGPAIAGMLFLASDDFAPALLGAGGGWLLSLLPLTMIQMKPGN
                     PIALRRTFPIEEIRNGWHLIRKRSTITKVLWCLIVATLLLGAVTPLITPLSRQFGLGS
                     EGTGVFFSALGFGTLIGPLIATGLFKRLHLSTTLLIVGLLAPVGLIVVGMLNHLQGVL
                     IAIAVAATAGTGLNVIVTTVTQRLSRSSHQGTVFGTQQTLLGIAWILALAATTGLTAL
                     WELESKVRMLFIALGGSGFLAILSCWTWNRQSIRKTCDLCEPELRIASVACRIVHGPP
                     YQISATLCGIICGEGCGRCYSQ"
     gene            complement(2749..4155)
                     /locus_tag="AC16V7_12510"
     CDS             complement(2749..4155)
                     /locus_tag="AC16V7_12510"
                     /inference="COORDINATES: protein motif:HMM:NF014296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HIR6257988.1"
                     /translation="MSTMRRFRAPLLPIDSPTGRERALIVIERLIKGALFKCSMCGNC
                     LLQETAFICPMLCPKGLRNGPCGSGAADGCCVDPSRPCVWHLIYQRAEAKGSLDKLLE
                     VQAPLDWTRVGHETWGTVLAEARSRGLFSPSFLAHADEWRGEVTELFRGIRQPEWWQG
                     DDQYHPPRSNGAASRLQAALDNGEFVLTAEVTPPADSNTEEVLQAAVGFRDVVHAANV
                     TQNPMATTRMSSLACSALLMEAGVEPVLQLTARDHNRFTLQSEALGATAFGINNILCL
                     TGDPPTKSRGPAAGLPFDLDATQMLWILRRMRDEAQLLDSRPLKKPPILFLGTAASPN
                     DPMPQHEARRLEKKINAGAQFIQTQLVYDTARLKRWLAALAERDLLQKVHILVGVGPL
                     RSVRMARYMREHIPDISIPPGLVDRMERSPDPKETGLEIALELVDQIKDLPGVSGIHL
                     MSLGWKSILPRLLKEARL"
BASE COUNT         1001 a         1134 c         1135 g          982 t
ORIGIN      
        1 acaaccaaga ttggtgagcg cgtctgccat tcccaagggg tggtcgatct ctcgggcaat
       61 agacagccca tgctcaatac gtgtgcgagc ggccgcgtaa tccccaaagc ccacgtcgac
      121 aattcccaat gaaatcagta cactggctcc ggctaccggc gcgttaccaa cgcgccggcc
      181 tgcgccgtgc tcgccagcct gcccaagggc tcggtggagg tatacgtcga aaactggaca
      241 gttggccaag tacgtttcgg ctgtggggca atcgggcgcc ttgaccctga caatagaagc
      301 caccttcgcc aaattggaga accgcaaacg taatctcctc aggcaggtct tgacagtctt
      361 ggactcgaag ccttcgatgg caagtcgttc cagcaggtcg ggtggtaagg tagaaccgcc
      421 ctggtaggtc atggtgtgct cctttcttgt gtggaaactt gaaggataca ccagacctac
      481 cgcttttttg ggcggttaga aattacagaa ggaatgatac gctactccaa aattcggctg
      541 tagcgtttcc cttaatggtc gcttcggttc aattgtcgac tcaagaattt ttctgggtct
      601 ttatcgaaca gtgctttgtg tgtggggttc atgaaataat attttttgcc cttgtactca
      661 gaactggggg cggtattttc attaaccatc atgccacaga ctggacatct caccattttg
      721 tctcacctcc ttccgaagaa tatattaaaa gctgtgttaa ggttagttgg cgggcagttt
      781 taggtcgtac ccaggaccga gcagtttaaa gtcttgccca ggactggcaa cagtagcctt
      841 tggaacagtg agcgaaccta gtgtacatcc aacatttctt gttcaccatg agtctcacct
      901 ccttaaacca tatcattcag gttctcctct catagatcgt ggacctcgtc ttgatgatgg
      961 cagattccag gctcttacgg gcgctgcgcc gccgcaaacc ctgtttcacc ttatttttac
     1021 ggcagcctct agcgatacgc cctgcgctaa tcaaaccccg gatgaagata ttattgctgg
     1081 gtctatagat atggacacca agctcaattg cacgccaatc gcgtgtatag ttgattattt
     1141 cttgcaaaaa agagagtcct ttatcatttt tataatcgct cattgtcttg ttcctcattg
     1201 gtcgggtttt actgggaata gcaacgcccg catccttcac cgcaaatgat gccgcacaaa
     1261 gtagccgata tctgatatgg gggaccatga acaatccgac aagcgactga agcaatgcgg
     1321 agttccggtt cgcaaaggtc gcaggttttc cgtatggatt gtcgattcca ggtccagcag
     1381 ctaaggatag ccaggaaacc ggaaccgcca agtgcgatga acagcatccg aactttcgac
     1441 tcaagttccc aaagcgccgt caggccagtg gtggcagcca gagccaatat ccaggcgata
     1501 cccaacaggg tttgctgggt gccaaatacc gtcccttggt ggcttgaccg ggacaaacgc
     1561 tgggtaactg ttgtcacgat tacgttcaga ccggtgcctg ctgtcgctgc gaccgcgatc
     1621 gcgatgagta caccctgcag gtggtttaac attccgacga caataagccc aaccggcgcc
     1681 aacagcccta caatcaacaa ggtggtggat aggtgcaacc gtttgaagag accagtggcg
     1741 atgagcggac cgatcagtgt gccaaacccc aaagcgctga aaaaaacgcc tgtaccttct
     1801 gaacccaggc cgaattgtcg agaaagaggc gtaatgagcg gtgtgactgc gcccaagagc
     1861 agcgttgcaa cgatcaggca ccataaaacc tttgtgatcg tactccgttt gcgaatcaga
     1921 tgccaaccat ttcgtatttc ctcaatggga aacgttcgcc gaagggcgat cggatttcct
     1981 ggtttcatct ggatcatggt cagcggtagc aatgagagca gccacccgcc gccggctccc
     2041 aaaagcgctg gagcgaagtc gtcggaggcc agaaacagca tccctgcgat ggcaggtcct
     2101 ataaagcttc ccacgccaga cagaatcgaa agggttgtgt tggcttccat gagccgagct
     2161 ctatcggtca attgggcaac caagcccaat cgggccggta taagcagcag gcggcctaac
     2221 atgatcagga acgcaatgat atagacgaac gtaacgatct ttgcgaaagt caggctgaaa
     2281 accagagcag caatggctga gtttgcgatg actgccgatc gcttaaatct caactgatct
     2341 gcaagtccgc ccatccagga accgaagaaa accggggcgg tcgactggat gatcatgaga
     2401 atgcctatgc tggaagatcc ccttccgagc aaatatgccc gctcaagaag gacgatgtag
     2461 acaatattgc atgccacttc agagatggca gaagaaaaca gtaaaagaga taatccccgg
     2521 cttggtgcca gcctcgccag gctaaggcgc ttggttgatc gcgccggtga ggtcgcatca
     2581 ggtggtcggc tctgttggaa gatctccgat acagtaaact tcatcattca actcttcccg
     2641 tttcgacggg tcagacttgc cgttggtcgc tgccaggctt gtactgatca tggatgtgtt
     2701 caatgtgctc agatgctgcg gtcctagatc tggctcccag gcttagattc aaagccttgc
     2761 ttccttcaga aggcgcggca ggatcgattt ccaaccaagg ctcatgaggt ggatgccact
     2821 tacgccgggc aaatccttaa tctggtcaac taactccaat gcgatttcca gcccagtctc
     2881 cttggggtca gggctgcgtt ccattcgatc gacgagccct ggcggaatgg agatgtccgg
     2941 aatgtgttca cgcatgtacc gggccatgcg aaccgaacgc agtgggccta caccaaccag
     3001 gatatgcacc ttttggagca agtctcgttc tgctagggcg gctaaccatc ttttcaacct
     3061 ggcggtgtca tagacaagct gagtctggat aaattgagct ccggcattga ttttcttttc
     3121 cagacgcctg gcttcgtgct ggggcatagg gtcattgggc gaggcggctg tccccaagaa
     3181 taggatcggt ggtttcttaa ggggtcgact gtccaggagt tgggcttcat ctctcattcg
     3241 ccgcaggatc cacaacattt gggtggcgtc aaggtcgaat ggcagaccag cagctggccc
     3301 ccggcttttg gtgggaggat cgcccgtcag gcacaggata ttgttaatcc cgaacgctgt
     3361 ggcgccgagc gcctcagatt gcagggtaaa gcgattgtga tctctggctg ttagctgaag
     3421 cactggttcc acacccgctt ccatgagcaa tgcactgcag gccaggctag acatgcgcgt
     3481 ggttgccata gggttctggg tcacgtttgc ggcatgaaca acatctctaa agccaaccgc
     3541 tgcttggaga acttcctctg tatttgagtc tgcaggcggc gtgacctcgg cggtaagaac
     3601 gaactcgcca ttatccagag ccgcctgcag ccttgatgca gcgccatttg aacgaggcgg
     3661 gtgatattgg tcatcaccct gccaccattc tggttggcgt attccgcgaa agagttccgt
     3721 cacttctcct cgccattcgt cggcgtgggc cagaaaactg ggagaaaaca gcccacgcga
     3781 gcgcgcctcg gcaagcactg tgccccaggt ctcatggccc actctcgtcc agtccagggg
     3841 agcctggact tccaagagct tatccagaga gcctttggct tcagcgcgct ggtagatcaa
     3901 gtgccagaca catgggcggg atggatctac acagcaacca tcagcagcgc cactgccgca
     3961 gggaccatta cgcaaccctt ttgggcacag catcgggcaa atgaaagccg tctcttgcag
     4021 cagacagtta ccacacatac tgcacttgaa gagcgctcct ttgatcaggc gctcaatcac
     4081 gatcaatgcc cgctctctcc cggtggggga gtcgatggga agcaacggcg ctcgaaatcg
     4141 tctcatggtt gacatattaa tgctcactta ttttagtaga ggcgctcatc gggttgcggt
     4201 catgtcctgg aatctcatgc cttgacgacc atttcctttg aggaatacga ag
//
LOCUS       DBNHOH010000719         1989 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1711357, whole genome shotgun sequence.
ACCESSION   DBNHOH010000719 DBNHOH010000000
VERSION     DBNHOH010000719.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1711357"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1310
                     /locus_tag="AC16V7_12515"
     CDS             <1..1310
                     /locus_tag="AC16V7_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR6257989.1"
                     /translation="YNPDDTFSHIVKLDEDAPLPVTKRIVAKLPPPTTNFIVPYIDTI
                     HNRIPVEIMRGCTRGCRFCHAGMITRPVRERTVDEVVNAIEQAIQKTGYEEVGLLSLS
                     SSDYTHVLELVNAVSQRFSEQHLSISLPSLRIESFSVELMDALKDARRGGFTLAPEAA
                     TERMREIINKPVSTEQLLETARAIYSRGWSTIKLYFMIGHPSETIEDVQAIADLCKAV
                     LAEGRKIIGRRAQVNAGVSTFVPKPHTPFQWVPCDSFEQILAKQALLKRSLRGPGLKL
                     TWNNPEDTMLEAWLSRGDRRMADVIYQAWKRGAKFDAWKEHFRYDLWLEAFESVGLDP
                     TFYTHRERPIDEIFPWEHINTTVRKKFLTEDYLWSLRGKTRIDCRERCFACGILPTFA
                     DLRRANPGDVWQCPEVKSKRIPVKVIQESNVEGSVKPDLITIQ"
     gene            1384..>1989
                     /locus_tag="AC16V7_12520"
     CDS             1384..>1989
                     /locus_tag="AC16V7_12520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HIR6257990.1"
                     /translation="MRLRITFAKTEAMRYTGHLDLHRTWERTLRRARLPLAYSQGFKP
                     HPRIVLASALPLGCTSQQEVVDIWLEKQESLSEIATRLNQAAPPGLGIIEVNEVAPNL
                     PSLPKQLEAAVFLITFLDPIPDLHSRLDQTNQAPEIPRVWRKKPYDLRALILDLQRLP
                     DDENGCQQILVKLAAREGATGRPEEVASVLGGEPSKVRIHRT"
BASE COUNT          502 a          553 c          491 g          443 t
ORIGIN      
        1 aatacaatcc cgatgacact ttttcacaca ttgtaaaact agatgaagat gcccccttgc
       61 ccgtcaccaa gcgcattgta gccaagctgc caccacctac caccaatttc atcgtgccat
      121 acattgacac gatccacaac cgcataccgg tcgaaatcat gcgcggctgc acccgaggct
      181 gccgattttg ccatgctgga atgattactc gcccggtacg cgagcgcaca gtggatgaag
      241 ttgtaaatgc cattgagcag gcgatccaaa agacaggcta tgaagaggta ggcttgcttt
      301 ccctttcgtc atcagactac acacacgtct tggaattggt aaacgcagtc agccagcgct
      361 ttagcgagca acacctgtcg atctcccttc cttccctgcg cattgaatca ttctccgttg
      421 agttgatgga cgccctgaag gatgctcgcc gtggagggtt tacgctagcc cccgaagcag
      481 caactgagcg tatgcgcgag atcatcaaca aaccagtaag cacagagcaa ttactggaaa
      541 ccgctcgagc aatctactct cggggatggt cgactatcaa gttatatttc atgatcgggc
      601 acccctccga gaccatagag gatgtccagg ccattgcaga cctgtgcaaa gctgtgctgg
      661 ctgagggacg aaagattatc gggaggcgcg cccaggtgaa cgccggagta agcaccttcg
      721 tcccgaaacc gcatacaccc ttccagtggg ttccttgcga ctccttcgag caaattcttg
      781 ccaaacaagc tctgttaaaa agatctttgc gcggaccggg actcaagctg acctggaata
      841 acccggaaga caccatgctg gaagcgtggt tgtcacgcgg cgaccggcgc atggccgatg
      901 tgatctacca agcctggaag cgcggcgcca agtttgatgc ctggaaagaa cacttccgtt
      961 atgatttatg gcttgaggca tttgaatcag ttggtcttga tcctacgttc tacacacatc
     1021 gcgagcgccc aatcgatgag atattccctt gggagcacat caacactaca gtccgcaaaa
     1081 agttcctcac cgaagattac ctgtggagtt taagaggcaa aactcgaatc gattgtcgag
     1141 aacgttgctt tgcctgcgga atcctgccga cgtttgctga tttgcgaagg gctaatccgg
     1201 gtgatgtctg gcagtgtcca gaagtaaagt cgaaaagaat cccggtgaaa gtaattcaag
     1261 agtcgaatgt tgagggtagt gtcaagcctg atctaataac cattcagtga ccaaccccgg
     1321 ttgcagttat tcaccgcccc taatttatct tagacctcaa ggttctttgt tcttccctta
     1381 agaatgcgcc ttcgcattac attcgccaaa actgaggcca tgcgctatac aggccactta
     1441 gatttgcacc gcacctggga gcgcacactg cgccgcgcca ggttgccgct agcctacagc
     1501 caaggattta aaccccatcc tcgcattgtc ctcgcctcgg ctctaccatt gggatgcaca
     1561 agtcagcagg aggtggtcga tatctggttg gagaagcaag aatcgttatc agagattgcg
     1621 acccgcttaa accaagctgc tccgccaggt ctggggatca tagaggtgaa tgaagtggct
     1681 cccaaccttc cctccctgcc aaagcaactg gaggcagctg tgtttttaat cacgtttctt
     1741 gaccccatcc ctgatctgca tagtcgtctt gaccagacta atcaagcgcc tgaaataccc
     1801 cgtgtctggc gaaaaaagcc ctacgacctg cgtgcattga ttttggatct ccaacgccta
     1861 ccagatgacg aaaacggttg ccagcaaata ctcgtcaaac tggcagccag ggaaggcgca
     1921 actggccgac cggaagaggt ggcgagcgtc ctcggcggcg aacccagcaa ggtccgtatt
     1981 caccgcaca
//
LOCUS       DBNHOH010000720         2987 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1455482, whole genome shotgun sequence.
ACCESSION   DBNHOH010000720 DBNHOH010000000
VERSION     DBNHOH010000720.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1455482"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..564)
                     /locus_tag="AC16V7_12525"
     CDS             complement(52..564)
                     /locus_tag="AC16V7_12525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR6257991.1"
                     /translation="MNVSEAIRMKRAVRQFQEKALPEEAILAILNAGRRSQSSKNTQP
                     WHFIAIRDKATLKALSEYGTWAGHLSGAPLGVAILHSDPSQRFQILFDIGQCAAYMQL
                     AAWEMGIGSCLVSIYEPEKARDLLGFPEDLHMRIGISFGYPLDPQDMSRLPQKGGRRS
                     PSEIYHWEKW"
     gene            complement(660..>2987)
                     /locus_tag="AC16V7_12530"
     CDS             complement(660..>2987)
                     /locus_tag="AC16V7_12530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR6257992.1"
                     /translation="DFGIIMAVVVVDAVLGFFQEYQAKKTYTALKSLIKPTTTVIRDG
                     QRQEVEVHELVPNDLVLLNAGEHVPGDGFLLESAKISVDEAILTGESEPVNKTATSSA
                     DVSAESLATYDNRLAMGTTVLTGRGLMRVTGTGSNTELGKIATSLQEHVEEETPLQVR
                     LKVFSKTLTRIVALFTIAILVSGLLLGRGFLDMLRTSIILAIAAVPEGLLIAVTVILV
                     LGMRKILKRNGLVKKLLAVETLGSVTTICTDKTGTLTEGRMRVTRTDLADPQRALETM
                     ILCNNLEGPVDIALWEYAKDQNQADPQDLVNTSERLAEELFSSETKYMSTYTRLNGER
                     NDYLKGAPEIVLEMCPISEIDKNQILARVDAWASEGLRLLGLAYRPHKNLEDHSDYNW
                     VGLLAMEDPIRDEVIEAVRIAHKAGIKIKMITGDYRRTAERIGRSIELLNGDEEILEG
                     GSLAAISDEELSKRVEKISIFARIRPNDKLRIIKALQANGEITAMIGDGVNDAPALKR
                     ANIGVVVGSATDVAKETADLILMDNNFRTIVAAVEEGRIIFENIRKVVAYTLSNSFAE
                     VLTIFIAMMLRWPAPLAVAQILWIHLICDGPSDIVLGFEPKEDGIMDKKPMSIKEPIL
                     TPLGMSLIAVISIASTVTALLLFDHFFQMHNNPVEGRSIIFASFAVNSMIYIFAYRSM
                     HQSIFRMRPLIENKPLIWAVAAGLLMAVLPFLVPSLQRLLGIVPLSLSEWALVAGVAL
                     GLLLIVELGKWISNYFYNQSESREMRGNQPVGVQR"
BASE COUNT          697 a          788 c          752 g          750 t
ORIGIN      
        1 aggcacaatt tagttgttaa ccaaggttcg accactcttg gttaaggcga gctaccattt
       61 ctcccaatga taaatttcgc ttggtgaacg cctgccccct ttttgtggca gtctgctcat
      121 atcctgggga tcaagtggat agccaaacga gatgccgatg cgcatgtgta gatcctccgg
      181 gaaaccaagc aagtcacgtg ctttctctgg ttcgtagatg ctcaccaggc aagagccgat
      241 tcccatctcc caggcagcta actgcatgta ggccgcgcat tgtccgatat caaaaaggat
      301 ttgaaagcgc tggctgggat cagaatggag aatcgccaca cccagcggcg cgccagacag
      361 gtgaccagcc caggtaccat attccgagag tgctttgagt gtggctttat cgcgtatggc
      421 gataaaatgc cagggttggg tgttcttgga tgattgcgag cggcgacccg cgttcagtat
      481 agccaggatg gcctcttcgg gcaaagcttt ctcctgaaac tggcgaacag cgcgcttcat
      541 gcggatggct tcggagacat tcatgtttta gaacctccac cactattggt ctggaataat
      601 caaatatatg ctgatcagag aaaagcactc atttcagcgc tcgtatctca gcaaatcaac
      661 tatctttgca ctccaacagg ctggttaccc cgcatctccc tggattcgga ctggttgtag
      721 aaatagttgc tgatccactt gcccaattca acgatcagaa ggaggccgag tgctacaccc
      781 gcaacaagcg cccactcact caggcttagt gggacaatac ccagcaggcg ttgcaaggag
      841 ggcaccagga aaggcagcac tgccatcaac aggcctgccg ccactgccca gattaagggc
      901 ttattttcga tcagggggcg catgcggaat atcgactggt gcatgctgcg ataagcaaag
      961 atatagatca ttgagttgac cgcgaagctg gcaaagatga tactgcgtcc ctccactgga
     1021 ttgttgtgca tctggaaaaa gtgatcgaaa aggagcaaag cggttactgt actcgcaatg
     1081 ctgatcaccg cgatgaggga catacccaac ggggtcaaga taggttcttt aatcgacata
     1141 ggcttcttgt ccataatgcc atcttctttg ggttcgaagc ccagcactat atccgatggc
     1201 ccatcgcaaa tcaagtggat ccaaaggatt tgagctaccg ccagtggcgc cggccagcgt
     1261 aacatcatcg cgataaagat agtcaacacc tcggcaaatg agttggaaag ggtgtaggct
     1321 actactttgc gaatattctc gaaaattatc ctgccttctt ccacggctgc tacaatagtg
     1381 cggaagttgt tgtccatcag gatcagatca gcggtttcct tggcgacatc cgtcgcgctg
     1441 cccaccacga caccaatgtt cgcccgtttg agtgctgggg catcgttgac cccatcgccg
     1501 atcatagcgg tgatctcgcc attggcttgc aaggctttga taattcgcaa tttatcattg
     1561 ggccgtatgc gggcgaagat agatatcttt tctacccgct tagagagttc ctcgtcggag
     1621 attgcggcta agctcccacc ttcgaggatt tcttcatccc cgttcaacaa ctcaatactt
     1681 cgcccgatgc gctcagctgt gcgacggtaa tcgccggtga tcatcttgat tttgatgccc
     1741 gctttatgcg cgatgcggac agcttcaatg acctcatccc gaattgggtc ttccattgcc
     1801 agcagcccca cccagttata gtcgctatga tcttctaagt ttttatgagg tcggtacgct
     1861 agaccgagca ggcgcagccc ttcactggcc cacgcgtcga ctcgggccaa tatttggttt
     1921 ttatcgatct ccgaaatggg gcacatttcc agcacaatct ctggggcccc ctttagatag
     1981 tcgttgcgct caccgttcaa gcgagtgtac gtgctcatgt actttgtttc gctgctgaaa
     2041 agctcttctg ccagccgttc agatgtgttc acaagatctt gtggatcggc ctgattttga
     2101 tcttttgcgt actcccacaa tgcaatatca actggacctt ccaggttgtt gcacaggatc
     2161 attgtctcga gagcgcgctg gggatcggct aaatcagttc gagttacgcg catgcgtcct
     2221 tctgtcagcg tgccagtctt gtcagtgcaa attgtggtca ctgaccccag ggtttcaact
     2281 gcaagtaatt tcttgaccag gccattgcgt ttgagaattt tacgcatgcc cagcaccaga
     2341 atgactgtga cagcgatgag caggccttcg ggaactgcgg cgatagccag gataatcgat
     2401 gtgcgcagca tatccagaaa gccgcgcccg agcaataagc ccgagaccaa gatagcaatc
     2461 gtgaagagag ccacgatgcg cgtcagtgtt ttactgaata cctttaaccg cacctgtagt
     2521 ggtgtttcct cttctacgtg ctcctggagg ctggtcgcaa tcttccctaa ctcggtgttt
     2581 gaacctgtgc cagtcacgcg catcaatcca cggccagtca aaaccgtagt ccccatagct
     2641 aagcggttgt catacgtagc tagcgattca gcagaaacat ccgcagatga ggtggctgtc
     2701 ttattcaccg gttcgctctc gccggtgagt attgcttcgt caacggatat ttttgcgctc
     2761 tccaggagaa acccatcgcc tgggacgtgc tcacctgcgt tgaggagaac caggtcgttt
     2821 ggtaccaact cgtgcacttc cacttcttgg cgttgcccgt cacggattac ggtagtggtg
     2881 ggtttgatca agctttttaa agcggtatag gttttctttg cctgatattc ctggaaaaac
     2941 ccgagaacag catccacaac gacgacagcc atgatgatac cgaaatc
//
LOCUS       DBNHOH010000721         6458 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_108270, whole genome shotgun sequence.
ACCESSION   DBNHOH010000721 DBNHOH010000000
VERSION     DBNHOH010000721.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6458)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6458)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6458
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_108270"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /locus_tag="AC16V7_12535"
     CDS             complement(<1..1288)
                     /locus_tag="AC16V7_12535"
                     /inference="COORDINATES: protein motif:HMM:NF047832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HIR6257993.1"
                     /translation="MARRYALVIGNSQYEDTTLSQLKTPEADVHALASALRDPQIGGF
                     DEVQEMINQSESAVRRAISAFFLQKKSDDLLLLYFSGHGVLDPQGRLFLAVKDSQRNL
                     LNATAIPANFITDDMDSCRSKRQILILDCCHSGAFAKGAKGDAPAVTQSTFEGNGYGR
                     VVLTASDSTQYALEGDQVIEQASFSLFTHYLLEGLISGEADIGLDGLITLDEWYDYAY
                     DRVVGETPEQTPRKWVYNQQGELVIARNPNPPQLGPEDLPEVLKLAIQSPFVAVRADA
                     VEELKRLLLGSNPAISQAAFQALENLAAHDDSKRIASAAGDALAALRQPAQPDRSEAE
                     HQEVEKVDQERQVREKAHVEPQKAEKADAERQDSAIDSQERLAEEAPISQQVTYLPFS
                     SELRPQKVNAGEDAEVLIEVSGQDTVSFSVDFVDPSG"
     gene            complement(1315..1707)
                     /locus_tag="AC16V7_12540"
     CDS             complement(1315..1707)
                     /locus_tag="AC16V7_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257994.1"
                     /translation="MAVEEAFQMTVQIALEDWDDEEVHSLAVQLAGELGDQPVESVEL
                     ARAGAAPVGTKSPEAITLGAVAVAVLPSVLPKVVEFCQAWALRGQGRTVKFEGKIHGQ
                     DIKFEGTADELQRLLAQLSSPPPAGNPA"
     gene            complement(1788..2804)
                     /locus_tag="AC16V7_12545"
     CDS             complement(1788..2804)
                     /locus_tag="AC16V7_12545"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR6257995.1"
                     /translation="MQRQSVIFSAPGQVAICDEPLADPAPNQVLVKTLCSAISPGTEL
                     LVYRGQIPEQLPLDVTIPSLAQEARYPLRYGYSCVGQVVAFGSQVERQWEGRHVFAFQ
                     PHTSHFLAEPQELMPLPTGIEPENAIFLPNMETAINFCMDGAPLIGERLAVFGQGIVG
                     LLTVMLLAQYPIHNLVTLDCYPLRRKVSIEIGAHASLDATEGDVLEQLRGIQPGGADL
                     VYELSGSPIALDQAIAATAFSGRVVVGSWYGKKRIELDLGGHFHRNRIRLLSSQVSTI
                     APELSGRWTKKRRFELAWEMIRRLQPKRFVTQYIALNDAPAAYHLLDQQPEETIQVVL
                     DYPR"
     gene            complement(2823..3521)
                     /locus_tag="AC16V7_12550"
     CDS             complement(2823..3521)
                     /locus_tag="AC16V7_12550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HIR6257996.1"
                     /translation="MVPIDELLAGAQKHRHNTGRPLVTLSYAQSLDGSITAERGIPLR
                     LSSPASMEMTHHLRAAHDGILVGIGTLLADDPRLNVRLVQGRDPQPVVLDSRLRFPLD
                     ANLLKGNRPPWIASLPGTDPQKRVELEKHGATILSLAEGGDGRVSLPDLLTCLADKGL
                     NSLMVEGGASVITSFFAQGLADRLVLTIAPVIIDGLRAVEPEASLASLPRLENAGFER
                     LGGDMIVWGALSYP"
     gene            complement(3508..4152)
                     /gene="ribA"
                     /locus_tag="AC16V7_12555"
     CDS             complement(3508..4152)
                     /gene="ribA"
                     /locus_tag="AC16V7_12555"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020481345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HIR6257997.1"
                     /translation="MDSLIVEHKACARIPTDMGEFQLNLYITNQDSKEHLALVMGEVR
                     DQSDVLVRVHSECFTGDVLGSRRCDCGEQLQHSMQMIAATGAGVLIYLRQEGRGIGLM
                     DKLRAYNLQDQGYDTVDANLALGHQADERDYSVAAGILDDLGVRSIRLLTNNPVKIQH
                     LQDLGVPVLERVPLHSTVNPDNAQYLFTKALRMNHLLDLEAFPVGATNRKNGSH"
     gene            complement(4157..4489)
                     /locus_tag="AC16V7_12560"
     CDS             complement(4157..4489)
                     /locus_tag="AC16V7_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6257998.1"
                     /translation="MNTGQTKFGAMQIGIILLALATAVIHIILAIPENMVMFYLNGVG
                     YLALTAALYLPQFEGMRGKVRWALIGYTAVTVIGWVAFGERNPVAYIDKIIELALIAL
                     LWVEGRQK"
     gene            complement(4507..5520)
                     /locus_tag="AC16V7_12565"
     CDS             complement(4507..5520)
                     /locus_tag="AC16V7_12565"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR6257999.1"
                     /translation="MKKHPWLQLLWLLVPVLVIWSVRDAPLADILRALSSLSVAKILL
                     LAALNTIILLLVSGRWWLILRAQGHTIRYLSVAAYRLVAFGISYFTPGPQFGGEPAQV
                     YYMRNRHGISTSSALASVSLDKILELLANFGFLLVGIFAALGSGLLVELAPIQAVAWM
                     AGPFILLLTYTLAIWKGKSPLASLLTRLPQRRGAILRLTRATALAEEQISQFCQRKPL
                     VVLGASVLSILIWGVMTLEYWLALRFLGIQVDLLQAVAALTAARIAFLFPLPGGLGAL
                     EASQILAMEALGLNPALGLSISLLIRGRDITLGGLGILLGGIMTRRRARVVVPNQATE
                     RRI"
     gene            complement(5532..6446)
                     /locus_tag="AC16V7_12570"
     CDS             complement(5532..6446)
                     /locus_tag="AC16V7_12570"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR6258000.1"
                     /translation="MAEHSIFGYPSQLDTLIQVNIRDFLVAFGLEKLRRGRKVLETVC
                     WLPAQRFASQMVEFDRQVGEQGLHQASRHTLKGYVSQLEIVGQQNIPASGPLLVLSNH
                     PGMTDTLALFASLDRPDLRIIAAERPFLSALSNVSRHLIYVPDDTNQRMGVVRNVVNH
                     LRRGGAALTFPAGEIEPDPYCMPGAVDSLEGWSESMAVFARLVPETSIVVAIVSGVIW
                     PASLSHPLTRLRKAQQDRERIAASLQVLGQMLSPSLRPVAVRVAFSQALPAAELINDG
                     SMTFMNAVTEQARQLIQKAPKFSEIYRG"
BASE COUNT         1496 a         1880 c         1738 g         1344 t
ORIGIN      
        1 ccccgctcgg atctacaaaa tccactgaga aagataccgt atcctggcct gaaacctcga
       61 tcaaaacctc agcatcctcc ccagcattga ccttttgggg gcgcaactca ctgctgaaag
      121 gcaggtaagt aacctgttgg ctgatgggcg cttcctcagc caggcgctct tgcgagtcta
      181 ttgcgctatc ttgacgctct gcatcggctt tttccgcttt ttgaggttca acatgcgcct
      241 tttctctaac ctggcgctcc tgatcgactt tttctacctc ctggtgctca gcttcagatc
      301 gatccggctg agcaggctgc cgcaatgccg ccagggcatc cccggccgcg ctggcaatcc
      361 gcttgctgtc gtcatgggct gccaggttct ccagagcctg gaaggcggcc tgcgaaatcg
      421 ctggattgct gccgagcaag aggcgcttca actcctctac ggcgtccgcg cgcacggcta
      481 caaatgggct ctggatagcc agcttgagca cctcgggcag gtcctcgggg cctaactgcg
      541 gggggttggg gttgcgggcg atgaccagct cgccttgctg gttgtagacc cacttacgag
      601 gggtctgctc aggcgtctcc cccactacgc gatcgtaggc gtaatcgtac cactcgtcga
      661 gggtgatcaa gccatccagg ccaatatccg cctctccgct gatcaagcct tccagcaggt
      721 aatgggtgaa gagcgaaaac gaggcttgct cgataacctg gtcaccctcc aatgcgtact
      781 gggtggagtc ggaggcagtg aggacaactc tgccatatcc attgccttcg aaggtagatt
      841 gggtgacagc cggggcatct cccttagcac ccttggcaaa cgccccacta tggcagcagt
      901 ccagaatgag gatttggcgc ttggagcggc aactgtccat atcatcggtg atgaaattag
      961 ccggtattgc agtggcattg agcagattgc gctggctatc cttcactgcc aggaacaggc
     1021 gcccctgggg atccagcaca ccgtggccgg aaaaatacaa caggagcaag tcatcagatt
     1081 tcttctgcag gaaaaaggcg ctaatagcgc gtcgcacagc ggactcagac tggttaatca
     1141 tctcctggac ttcatcgaag ccaccgattt gcgggtcgcg caaggcagac gccagggcgt
     1201 gcacatcggc ctcgggtgtt ttcagttgag acagagtggt gtcttcgtac tggctgttgc
     1261 ctataacaag tgcgtatctc cgcgccatgc aacctccgcg gttaaccaga gagactaggc
     1321 tggatttcca gcgggtggcg ggctggagag ctgcgccagg aggcgctgga gttcgtcagc
     1381 cgtcccttca aatttaatat cctgcccgtg gattttgccc tcaaacttaa ccgtgcgccc
     1441 ctggccgcgc agcgcccagg cttggcagaa ttccaccacc ttgggcaata cgctgggcaa
     1501 cacggctacc gccactgccc ctagcgttat cgcttcgggc gacttcgtgc ctacaggcgc
     1561 agcgcctgcg cgagctagct caaccgattc gacaggctga tcaccaagct cgccagctag
     1621 ctggacggca aggctgtgca cttcttcatc gtcccaatct tccagggcaa tttgtactgt
     1681 catttggaat gcttcttcta ctgccattcg gaatccccgt tcttataatg atgaccaata
     1741 ttgtctagat ataattagtt tacattaaaa gatccgaatt ggcaacctca gcgggggtaa
     1801 tccagcacca cctggatggt ctcttcaggc tgctgatcca acaagtgata agctgccgga
     1861 gcgtcattca aagctatata ttgagtgaca aaacgcttcg gctgcaagcg gcggatcatc
     1921 tcccaggcca attcaaaacg gcgcttcttg gtccagcgtc cgctcagttc cggggcgatc
     1981 gtgctgacct ggctgctgag caggcgaatg cggttgcgat gaaaatgccc gcccagatcc
     2041 agctcgatcc gcttctttcc ataccaggag ccgactacca cccgcccgga gaaagccgta
     2101 gcagcgatag cctggtcgag ggcaatagga gagccggata gctcatagac caaatccgca
     2161 ccgccaggtt gtataccccg caactgttcc agtacatcac cctctgtagc atccaggctg
     2221 gcgtgcgccc cgatctctat ggagacctta cgccgcagcg ggtaacaatc caatgtgacc
     2281 aaattatgga tggggtattg cgccagcaac atcacagtca aaagaccgac aatcccctgc
     2341 ccaaagactg cgaggcgctc gccaatcagc ggggcgccat ccatgcagaa attgatcgcc
     2401 gtctccatgt tgggcaagaa gatcgcattt tctggctcaa tgcctgtggg tagcggcatc
     2461 aactcctgag gctcagctag aaaatggctg gtgtgaggct ggaaagcgaa aacatgtcgc
     2521 ccttcccact ggcgctcgac ctgcgaccca aaagccacca cctgtcctac acaggagtag
     2581 ccatagcgca gggggtagcg ggcttcctgc gccagggatg ggatggtcac atcaagcggt
     2641 aactgctcgg ggatttggcc gcgataaacc agcaattcag tgcccgggct tatcgccgag
     2701 cacaaggtct ttacgagcac ctggtttgga gctgggtcag ccagcggctc gtcgcagatc
     2761 gcgacctgcc cgggagcgga gaaaataaca gattggcgtt gcatgagatg atgaattatt
     2821 gtttacggat agcttagtgc gccccagacg atcatatcgc caccaagacg ctcaaaaccc
     2881 gcgttctcca agcgggggag agaggccagg gatgcctcag gttccacagc acgcagaccg
     2941 tcaataatga ccggggcaat cgttagcacc agtcggtcag ccaatccttg tgcaaagaaa
     3001 ctggtaatga cacttgcccc gccctcgacc atcaagctat tcaagccctt atcagccagg
     3061 catgtaagca gatcaggcaa ggagacacgc ccatcgccgc cttccgccag gctcagaatg
     3121 gtcgccccgt gcttctctaa ttctacccgt ttttgcggat ctgtaccggg cagggaagca
     3181 atccacgggg gtcggttgcc ctttaatagg ttggcgtcaa ggggaaaacg caggcggcta
     3241 tccagcacca ctggctgcgg gtcgcggccc tgcacaaggc gcacattgag gcgggggtca
     3301 tctgccagca gcgtgccaat gcccaccagg atgccgtcat gagccgcgcg cagatgatgg
     3361 gtcatctcca tcgaggcagg gctgcttagt cgcaagggaa tgccccgctc tgctgtaatg
     3421 cttccatcca ggctttgggc gtaactcagt gtgacgagcg ggcgacctgt attgtggcgg
     3481 tgcttctgcg ctcctgccag caactcgtca atgggaacca ttcttacggt tcgtcgcccc
     3541 cacaggaaag gcttccaggt caagaagatg gttcatacgc aaggctttcg tgaacaggta
     3601 ctgggcgttg tcggggttga ccgtggaatg cagcggcacg cgctccaaga caggcacacc
     3661 caaatcttgc aggtgctgta tcttaaccgg gttattggtt agcaagcgga tggagcgcac
     3721 gcccaaatca tccagaatac ctgccgccac gctgtaatcc cgctcatcgg cctggtgacc
     3781 cagagccagg ttagcatcca ccgtgtcgta tccctggtct tgcaggttgt aggcgcgcaa
     3841 cttatccatc aggccgatac cgcgcccctc ttggcgcagg tagatcaaca cgccagcccc
     3901 agtagcagcg atcatctgca tagagtgttg caattgctcg ccgcagtcgc agcgccgcga
     3961 accgaggaca tccccggtga aacattccga gtgcacccga acaagcacat cgctctgatc
     4021 gcgcacttct cccatcacca aagccaagtg ttctttgcta tcctggttag tgatgtataa
     4081 attgagctgg aactcgccca tatccgttgg aatgcgggcg caagctttgt gttcaacaat
     4141 caagctatcc atgatactac ttttggcgtc cttcgaccca caacaaggca atcagcgcta
     4201 attcaataat tttgtcgata taagccaccg ggtttcgctc cccaaatgca acccagccga
     4261 tcaccgttac agcagtgtag cctatcagtg cccagcgcac cttaccacgc atcccctcaa
     4321 actgaggtaa gtatagggca gcagtcaatg ctaaataccc gacgccattc aagtaaaaca
     4381 tcaccatgtt ctcaggtatt gccaggatga tatgaataac cgcggttgct agagcaagca
     4441 gaataattcc aatctgcatc gccccaaatt tggtttgtcc tgtgttcatt ttatattcct
     4501 ccttagttat attcttctct ctgtggcctg gttaggcacg acaacacgag cgcgccggcg
     4561 cgtcatgatg ccgcctaaca aaatacctaa accacctaat gtgatgtccc gcccccggat
     4621 gagcaggctg atgctcaaac ccaaagccgg gttgagtcca agggcttcca tcgcaagtat
     4681 ctggctagcc tccagcgcac ccaacccccc cggcaatggg aacaagaaag caatgcgggc
     4741 agcggtcagg gcggcaactg cctggagcaa gtctacctgg atgcccagga agcgcaaagc
     4801 cagccaatac tccagggtca tcacgcccca gatcaagatg gatagcacag aggctcccag
     4861 cacgacgagc ggtttacgct ggcaaaactg gcttatctgc tcttcggcca aggcggtggc
     4921 gcgcgtcaat cgcaggatcg ctccgcgccg ctggggtaag cgagttaaca agcttgccag
     4981 gggggatttc cccttccata tcgccagcgt ataggtcaac aacaagatga atggccccgc
     5041 catccatgcc acagcctgaa tgggcgccag ctctacgagc aaaccggatc ccaaggctgc
     5101 gaatataccg accagcaaga agccgaaatt tgccagcaac tctaggattt tatccagcga
     5161 tacggacgcc agcgcactgg aagttgagat accgtggcgg ttccgcatat agtagacctg
     5221 cgctggctct ccgccgaact gtggtccagg tgtgaaatag ctgatgccaa aggctaccag
     5281 ccggtatgcc gccacggata aatagcggat ggtgtgccct tgggcacgca aaatcagcca
     5341 ccaacgacca ctgactagca agaggataat ggtgttgagg gctgccaata gcagaatctt
     5401 ggcgacgctc aaactgctca gggcgcgcag tatatctgcc agaggggcat cccgcacaga
     5461 ccaaatgacc agcacgggaa ccaggagcca aagtagttgc agccaagggt gctttttcat
     5521 gtattgcttt gctaccccct ataaatctca gaaaacttgg gggctttttg gataagctgg
     5581 cgtgcctgct cggtgacagc gttcatgaat gtcatagaac catcgttgat caactcagca
     5641 gcgggcaatg cctggctgaa agccacacgc actgcgaccg gtcgcagaga tggcgaaagc
     5701 atttgaccca ggacctgcaa gctggcagcg atgcgctcgc gatcctgctg cgccttgcgc
     5761 aagcgagtga gcgggtgact cagcgatgcc ggccagatga ccccactgac aatggcgacc
     5821 acgatgctcg tctcagggac caaacgggcg aagacagcca tgctctcaga ccagccctcc
     5881 agcgaatcaa cagctccagg catacagtag ggatcgggtt caatctctcc ggcggggaaa
     5941 gtgagtgctg caccaccgcg tcgcaagtgg ttaacgacat tacgcactac gcccattcgc
     6001 tgattggtgt catcgggcac ataaatcaag tgacggctga cattggacag agcactcaag
     6061 aaaggccgtt cggcggcaat aatacgtaga tcaggacggt ccaagcttgc gaataacgcc
     6121 agtgtatctg tcattccagg atggttagag agcaccagca gcggtccgct agccgggata
     6181 ttctgctgtc ccacaatttc taattgagat acatatcctt tcaaggtgtg ccgagaggct
     6241 tggtgcaatc cttgctcgcc aacctggcga tcgaattcaa ccatttgcga agcaaagcgc
     6301 tgcgctggca gccagcacac tgtctccaac acttttcgcc cgcgccgaag tttctccaga
     6361 ccaaaggcga ctaagaaatc ccgtatattg acctggatta acgtgtcgag ttgtgaaggg
     6421 tatccaaaaa tgctgtgttc agccattaaa ttacacta
//
LOCUS       DBNHOH010000722         2857 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_300908, whole genome shotgun sequence.
ACCESSION   DBNHOH010000722 DBNHOH010000000
VERSION     DBNHOH010000722.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_300908"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2084
                     /gene="nuoL"
                     /locus_tag="AC16V7_12575"
     CDS             <1..2084
                     /gene="nuoL"
                     /locus_tag="AC16V7_12575"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HIR6258001.1"
                     /translation="SHTVAVGAALLSWLGSMVVFFRSLQVEHFGEKIFASSIPWLPTG
                     DSWFRIGVQIDPLSAATLFFVAWTVLMIFLYSVGYHNFGQPKGDHDVAGLPPHGATVS
                     DAHGHTHKVPSIEPMYSRFFAFIGLFAFGMYTLVVSDNLLTLFVGWEIMGLCSYLLIG
                     FWYGKPSARAAMIKAFITTRVGDVFMLLGIAYLYSVAGTLNYREIFYNEEMLAHLAEM
                     PSYIVGLSAAGLIGILLFIGTIGKSAQWPLHVWLPDAMEGPTPVSAMIHAATMVSAGV
                     YMAIRIFPLLSAGSEHGATSAMMLVSFIGAFTALFAATIAVAQNDIKRVLAYSTISQL
                     GYMVAALGIGAYVAAAFHLITHAFFKALLFLGSGSVIHGMEHGVLHTGDHIDPQNMFN
                     MGGLRKKMPVTFWTFLIGGFALSGFPLVTAGFWSKDEILADAWGHGHVVVFGTLALAA
                     LLTAFYTMRQITLTFLGKPRTEAAHHAQETPWTMTAPLAILAVFSVAAGWVGIPEHFP
                     VLGGLLPNWFHEFLGGTLLEHPEAVEFNLVPLLTSLVVALGGLYMGWLVYRETPAGAQ
                     DPIEVTVPSMHKLLQNKYYFDEIYDRIFVRPAYWFSETFTSAWMDQGVIDGVLHWVAM
                     VSGTIGGTLREYIDKRIVNASGDIVGEGIKKFGRAFRVIQTGRIQQYMIGALVALAFF
                     SALFYYLIFTP"
     gene            2117..>2857
                     /locus_tag="AC16V7_12580"
     CDS             2117..>2857
                     /locus_tag="AC16V7_12580"
                     /inference="COORDINATES: protein motif:HMM:NF012581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter transmembrane
                     domain-containing protein"
                     /protein_id="HIR6258002.1"
                     /translation="MDFIASHLLTLILFTPVLAAVILFFLPREQEDLLRWTAFVLSFV
                     PLAFSLALWFGFDAGKPGFQYEERAVWYAAINSSYHLGVDGISLTMVLLTTLLTPLAI
                     LASFTIKDNIKAYMILFFLLEMGMLGVFLSLDLLLFFVFWEFGLVPMYFLISQWGSEK
                     GERQIWGGRTVSARAYASFKFMIYTMAGSLGLLLAIQVIGVTARTYDMTQLFQIWPGL
                     QSLNDLPLNLPVSTVKAVAFWAFAIAFAI"
BASE COUNT          517 a          780 c          776 g          784 t
ORIGIN      
        1 tcagccatac agtcgctgtt ggagcagcgt tgctctcctg gttgggcagc atggtggtgt
       61 tcttccgctc gctgcaggtt gaacactttg gagagaaaat cttcgcctcc tccatcccct
      121 ggcttcctac gggtgattcg tggttcagga ttggcgtgca aattgatccg ctctctgcag
      181 caaccctgtt tttcgtcgcc tggaccgtgc tgatgatctt cctgtacagc gtgggttatc
      241 acaactttgg ccagcccaag ggtgaccacg acgttgccgg tcttccaccg catggcgcta
      301 cggttagcga tgcgcacggg cacacgcaca aagtcccatc catcgagccg atgtactcac
      361 gcttttttgc cttcatcggt ctctttgcct ttggcatgta taccctggtg gtctctgata
      421 atctgcttac ccttttcgtt ggctgggaaa tcatgggact ttgctcgtac ctgttgattg
      481 gtttctggta cggcaagcca tctgctcggg cagcgatgat caaggcattt atcaccacgc
      541 gcgttggtga tgtctttatg ctgttgggca tcgcctacct gtactcggtg gctggcacgt
      601 tgaactatcg tgagattttt tataacgaag agatgctggc acacctggct gagatgccct
      661 cctatatcgt gggtctatca gcagccgggt taattggcat cctgctgttc atcggcacga
      721 tcggcaagtc tgctcaatgg cccctgcatg tatggttgcc cgatgccatg gaaggcccta
      781 cgccggtctc agcaatgatc catgctgcca cgatggtttc cgcgggcgtc tacatggcga
      841 ttcgcatctt cccgctgctc tccgccggtt ccgagcatgg ggcgacctca gccatgatgt
      901 tggtgtcgtt catcggggcg ttcactgcct tatttgccgc caccatcgcg gttgcccaaa
      961 acgatatcaa gcgcgtgctg gcatattcga cgatttcgca actggggtat atggtggctg
     1021 ccctgggtat cggcgcttac gtggcggcag cattccatct gatcacgcat gccttcttta
     1081 aggcactgct cttcttgggc tctggctcgg tgatacacgg catggagcat ggtgtcctgc
     1141 ataccggcga ccacatcgac ccgcagaata tgttcaacat gggcggcctg cgcaagaaga
     1201 tgccggtgac tttctggaca ttcttgatcg gtggcttcgc cctctccggc tttcctttgg
     1261 tgacagccgg attctggtca aaagatgaaa tcctggcaga tgcctgggga cacgggcatg
     1321 ttgtggtctt tggcacctta gcactggcgg cattgctcac ggctttttac accatgcgcc
     1381 agatcaccct gaccttcttg gggaagccgc gcaccgaagc tgcccaccat gctcaggaga
     1441 ctccctggac gatgaccgca ccattggcta tcctggcggt attttctgtt gcagccgggt
     1501 gggtgggcat cccagagcat ttcccagtcc tcggagggct gctgcccaac tggttccacg
     1561 agttccttgg aggaactttg ttggagcatc cagaagcggt cgaattcaac cttgtcccac
     1621 tactcacttc gctggtcgtt gcgctgggcg gattgtatat gggctggttg gtttaccggg
     1681 aaacacctgc cggggcgcaa gatccaatcg aagtaactgt gcctagtatg cacaagctct
     1741 tgcaaaacaa gtactacttt gatgagatct atgatcgaat tttcgtgcgc ccagcctatt
     1801 ggttctcaga gacatttact tcagcttgga tggatcaagg tgtgatcgat ggcgttttgc
     1861 actgggtggc gatggtctcc ggaacgattg gcggcacgct acgcgaatac atcgataagc
     1921 gcattgtgaa tgcctctggt gatattgtgg gtgagggcat taagaagttt ggtcgtgcat
     1981 tccgcgtcat ccaaaccggg cgcattcagc agtacatgat tggggcattg gtcgcgcttg
     2041 ccttctttag cgcgttgttc tattacctga tctttacccc ctgacgttcg tcacatcgga
     2101 cctacaggag cgaacgatgg attttatcgc aagtcacttg cttacactta tcttgttcac
     2161 accggtgctg gcggcagtga tcctgttttt cctgccgcgc gagcaggaag acctgctgcg
     2221 ctggacagca tttgtgctca gctttgtacc cctagcattt tccttagccc tgtggttcgg
     2281 gtttgatgcc gggaagcccg gtttccaata cgaggagaga gccgtctggt acgcggcgat
     2341 caactcttcg tatcaccttg gtgtggacgg catttccctg accatggtct tgctgactac
     2401 tttgctcact ccactggcaa tcctggcatc ctttaccatc aaagacaata tcaaagcata
     2461 tatgatcctg ttcttcctgc tcgagatggg catgttgggc gtctttctat ccctggatct
     2521 gctgctcttt ttcgtctttt gggagtttgg cctggtaccg atgtacttcc tcatcagcca
     2581 gtggggcagc gagaaaggcg aacgccagat ttggggcggg cgtacggtct cagcccgtgc
     2641 ctacgcctca ttcaaattca tgatctacac catggcgggc tctttgggac ttctgctggc
     2701 gatccaagtg atcggtgtga ccgctcgaac ctacgatatg acccagctat ttcagatctg
     2761 gccgggcttg caatctttga acgatctacc gctcaacctg ccggtttcta cggtcaaggc
     2821 ggtggccttt tgggcatttg ccattgcttt cgccatc
//
LOCUS       DBNHOH010000723         1604 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1711525, whole genome shotgun sequence.
ACCESSION   DBNHOH010000723 DBNHOH010000000
VERSION     DBNHOH010000723.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1711525"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="AC16V7_12585"
     CDS             complement(<1..382)
                     /locus_tag="AC16V7_12585"
                     /inference="COORDINATES: protein motif:HMM:NF012691.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HIR6258003.1"
                     /translation="MFDSEKVEKAAPTAFIIFGVTGDLTKRKLVPALYELALTQRLPD
                     AFYIIGFARRDWDNLTMAKTLTQNVSDFARTNTVDTKVLEALCSKMRYVQSTFEEEDG
                     YRRLGQILDELGIINRLYYLAAPPE"
     gene            complement(395..1087)
                     /locus_tag="AC16V7_12590"
     CDS             complement(395..1087)
                     /locus_tag="AC16V7_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258004.1"
                     /translation="MEYDNLHKSDTQPRLFSLGQASSGIAELFPEIWTASEDLSSPNP
                     TIRRLALEFLEHSGAARISPLVVYIIATRLDDTDLVVRMQVLKILGDVLAPDELGNSP
                     PEMVTSYLTNNLSNMRTRQVYAILQALVQDINLDLQVIRILKTCPYAGNHLMEMANSR
                     KVPLNMRRKAIWLVGQIGYLEAIPALERMQIRMETRLTGQQSMPFAPPIGIDDIDLLD
                     DVISTLGLLQSP"
     gene            complement(1110..>1604)
                     /locus_tag="AC16V7_12595"
     CDS             complement(1110..>1604)
                     /locus_tag="AC16V7_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase-like TK C-terminal-containing
                     protein"
                     /protein_id="HIR6258005.1"
                     /translation="KIAISRRAGPTALIFTRQNLPIIDRNIYGSASGVQQGAYVLADL
                     GDGAPELILMASGSEVSLIVQTGIILAAEGLNVRLVSFPSWELFNMQTKEYRDSVFPS
                     NIRSRLAVEAGVSQGWERWVGDQGIVLSIERFGASAPYQVIYEKYGLTVENIVTYART
                     MVSR"
BASE COUNT          431 a          394 c          348 g          431 t
ORIGIN      
        1 cctctggagg agcagccaaa taatataatc gattgataat ccccaactcg tccaatattt
       61 ggccaagccg cctgtagcca tcctcttctt cgaatgtcga ttggacatag cgcatcttgg
      121 agcataaagc ttccaggact tttgtatcta ccgtatttgt acgcgcaaaa tcgctcacat
      181 tttgggtgag cgtttttgcc atggtcaaat tgtcccaatc acgacgcgca aatccaatga
      241 tgtaaaaagc atctggaagc cgctgggtaa gtgcaagctc atagagcgct ggcacaagct
      301 tcctcttggt aagatcacct gttacaccaa agataataaa tgcagttgga gctgcttttt
      361 cgactttttc agaatcgaac atgggtaatg agaattaagg agattggagc aatcctaaag
      421 tggaaatcac atcgtctaat aggtcgatgt catcaattcc aatcggaggc gcaaatggca
      481 tgctttgctg accggttagg cgtgtctcca ttcggatttg catccgttcc aatgcaggaa
      541 ttgcctccaa atagcctatt tgaccaacca accatatggc tttacggcgc atattcagtg
      601 gtaccttgcg tgaatttgcc atttccatca gatgattacc agcatatggg caggttttta
      661 gaatacggat aacctgcaaa tctaaattga tatcttgcac caatgcttgc aaaatcgcgt
      721 atacctggcg ggtgcgcata ttgctcagat tgtttgtgag atagctggtg accatctctg
      781 gcggactgtt tcccaattca tccggggcaa gcacatctcc cagtattttc aacacctgca
      841 ttctcactac aaggtcagtg tcatccaggc gagttgcaat gatgtaaacc acaagaggag
      901 aaatccgcgc tgcaccggaa tgctctaaga attccaaagc tagacggcga atagtaggat
      961 ttgggctgga tagatcttcc gatgctgtcc atatttccgg aaataattcg gctattccag
     1021 agcttgcttg gcctaggcta aacaacctgg gttgtgtatc ggatttatgc agattatcat
     1081 attccatcga tagctacctt ccccgagact tatctggaga ccattgttct ggcataggtc
     1141 acaatatttt ctaccgttaa cccatatttc tcataaatga cttgatacgg agctgaggca
     1201 ccaaacctct caatcgagag gactatgccc tggtctccaa cccagcgctc ccaaccctgt
     1261 gagacacccg cctcgacagc cagccgtgaa cgtatattgg aagggaaaac cgaatcacgg
     1321 tattctttcg tttgcatgtt aaaaagttcc cagctaggga atgataccaa cctgacgttc
     1381 aagccttcgg cagccaggat tataccagtc tggactatta atgatacctc ggatcccgaa
     1441 gccatcaaaa ttaattcagg agctccatct cccagatcag ccaatacgta tgccccctgc
     1501 tgcacaccgc ttgcggaacc gtatatattt ctatcaatta ttggcaggtt ttgacgcgta
     1561 aaaatcaatg ccgtgggtcc ggcccgccgt gaaatagcta tttt
//
LOCUS       DBNHOH010000724         2489 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_429275, whole genome shotgun sequence.
ACCESSION   DBNHOH010000724 DBNHOH010000000
VERSION     DBNHOH010000724.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_429275"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /gene="iolB"
                     /locus_tag="AC16V7_12600"
     CDS             <1..892
                     /gene="iolB"
                     /locus_tag="AC16V7_12600"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HIR6258006.1"
                     /translation="NLVIRPYTSNDPSLIVDVDPAKAGWQYIHFQSRSLEAGSSWSFA
                     PGEYEIALVVLSGTLSVKSNRGEWSALGKRPSVFAGLPEALYLPRNSSLTVGADTDCQ
                     FAVAWAASSGDHPPRLITQEQVSVEIRGGDNVTRQINGIIRPGFDCDRLVVVEVYTPS
                     GNWSSYPPHKHDVHQVDQHGNLVQADLEEVYYYKIDKPEGYALQRIYTGESSPLQRAG
                     EIIDEVLMLRNDDLVLIPEGYHPVTSAPGYTTYYLNVLAGSAQALTAVDDPQYSWVKD
                     TYRSKDPRLPIYPVDPRQII"
     gene            948..>2489
                     /gene="iolD"
                     /locus_tag="AC16V7_12605"
     CDS             948..>2489
                     /gene="iolD"
                     /locus_tag="AC16V7_12605"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     carbon-carbon bonds, in ketonic substances [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HIR6258007.1"
                     /translation="MKTVRLITAQAIIRYLKNQFVERDGQKHQFFAGAWGIFGHGNVA
                     GIGQALLENPDFPFYFSRNEQAQVHLAAAYAKMKNRLGAFACTSSIGPGATNMITGAA
                     VATINRLPVLLLPGDIFARRNVAPVLQQLESGWSQDISVNDCFKPVSRYWDRINRPDQ
                     ILTALPEVMRVLTSPVDTGAVTLSLPQDVQTEGHDYPVEFMEPRTWYIPRQPPDSSLF
                     QRSAEWIRSSKAPLIIAGGGVHYSEAYAALAEFVHQTGIPVAETQAGKGSLLSDHPQL
                     VGAVGVTGTLVANRLARAADLVIGIGTRYSDFTTASKTAFENPDVRFINLNVAEFDAY
                     KHAALPLVADARLVLEGWSEALKGYQVSSAYAEQISQGRAAWDEEVDRLYHLGTSGLP
                     AQSEVIGALNEFLRPGDVIVNAAGSAPGDLHKLWRSRDHKSFSMEYGYSCMGYEIPGA
                     LGVKMAAPEREVYALVGDGTYLMLPSEIVTSIQEGYKLNIILIDNHGFASIGSLSRSL
                     GTAGFG"
BASE COUNT          523 a          701 c          702 g          563 t
ORIGIN      
        1 gaacctggtc atccgtccat atacgtccaa cgatccgtca ctgattgtgg atgtggatcc
       61 ggccaaagca ggttggcaat atatccattt ccagtctcgc agcctggagg caggcagttc
      121 ctggtcattt gcccctggtg agtatgagat cgccctggtc gtgctctctg gcacgctaag
      181 cgtgaagtcc aaccgaggag agtggtctgc cctgggcaaa cgccccagcg tgtttgccgg
      241 gctgccagaa gcgctttacc tgccccggaa ctcttccctt acagtcggcg cggatacaga
      301 ttgccagttt gctgtagctt gggcggcgtc ttcaggagac catcctcctc gcctcatcac
      361 acaggagcag gtcagcgtgg agattcgcgg cggagataac gtcacccgcc agatcaatgg
      421 catcatccgt ccaggtttcg actgcgaccg cctggtagtg gtagaggtgt atacacccag
      481 cggtaattgg tctagctacc ctccgcacaa acatgatgtc caccaggtcg accaacatgg
      541 caacctggtg caggctgacc tggaagaggt ttattactat aaaatcgata aacccgaagg
      601 ttatgctctc cagcgcattt acaccggaga aagctcgccg ctgcagcgag caggcgaaat
      661 aatcgatgag gtcttgatgc tgcgcaacga cgacctggtg ctgatccctg agggatacca
      721 tccagtgacc agcgcgccgg gctatacaac ctattatctg aatgtcctgg caggtagtgc
      781 ccaagcgctc accgcggtag atgacccgca gtacagctgg gtgaaggata cctatcgatc
      841 caaagacccg cgcctgccga tctatccggt tgatccgcgg cagattatct gaggagcgaa
      901 cctgtttctt gagttcgccg taattctgat cattagagga taagcaaatg aagactgtcc
      961 gactaattac tgcacaagcc attatccgct acctgaaaaa ccagtttgtt gaacgagatg
     1021 ggcagaagca tcaattcttt gctggtgcct gggggatttt tgggcatggg aacgtcgccg
     1081 gcatcggtca agcactgtta gagaacccgg attttccctt ttacttctct cgcaatgagc
     1141 aagcccaggt acacctggca gctgcttacg caaaaatgaa aaaccgtttg ggggcctttg
     1201 cctgtaccag ctccattggt cctggggcga ccaatatgat caccggcgct gcggtcgcta
     1261 cgatcaaccg tctgcctgtt ttgctgctgc cgggagatat cttcgcccgc cgaaatgtcg
     1321 ccccggtgct ccagcagctt gagtcgggtt ggagccagga tatttccgtc aatgattgtt
     1381 tcaaaccggt ctcacgctat tgggatcgca tcaaccgccc agaccagatc ctgactgccc
     1441 tgccagaggt gatgcgtgtg ctcacgtcgc cagtggatac cggtgcagtg actctctccc
     1501 ttccccaaga tgtccagacc gaaggtcatg actatccggt ggaattcatg gaaccccgga
     1561 cctggtacat cccacgccag ccccctgaca gcagcttgtt tcagcgctca gcggagtgga
     1621 tccggtcgag caaagcgccc ttgatcatcg ctggcggtgg cgttcactat agtgaggcgt
     1681 atgcagccct ggcagaattt gttcaccaaa cgggtatccc ggtggctgaa acccaggcag
     1741 gtaaaggctc gcttctaagt gaccaccctc aactggttgg ggctgttggg gtgactggca
     1801 cgctggtcgc caaccgctta gcgcgcgctg ccgacctggt catcggcatt ggcacccgct
     1861 attccgattt caccaccgcc tctaagaccg ccttcgagaa cccggatgtg cgtttcatca
     1921 acctgaacgt cgcggagttc gatgcctaca aacatgctgc cctgccgctg gtagccgatg
     1981 cccggctggt gctggaaggc tggtcagaag ctttgaaagg gtaccaggtc tcatctgcct
     2041 atgccgagca gatcagccag ggccgcgctg cctgggatga ggaggtagac aggctgtacc
     2101 acctgggcac gtctggactt cctgcgcaga gcgaagtgat cggcgcattg aacgagtttc
     2161 tgcgcccggg agatgtgatc gtcaacgctg ccggcagcgc ccctggagat ttgcacaaac
     2221 tctggcgctc gcgcgatcac aagagctttt caatggagta tggctattct tgtatgggct
     2281 acgagattcc gggcgcgctg ggggttaaga tggctgctcc ggagcgcgaa gtttatgccc
     2341 tggtgggaga tggcacttat cttatgctgc catctgaaat tgtcacttct atccaggagg
     2401 ggtacaagct gaacatcatc ctgattgata atcacggctt tgccagcatt ggctcactga
     2461 gccgctcgtt gggtactgcc ggttttggg
//
LOCUS       DBNHOH010000725         4362 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1326983, whole genome shotgun sequence.
ACCESSION   DBNHOH010000725 DBNHOH010000000
VERSION     DBNHOH010000725.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4362)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4362)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4362
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1326983"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..1171
                     /locus_tag="AC16V7_12610"
     CDS             185..1171
                     /locus_tag="AC16V7_12610"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR6258008.1"
                     /translation="MSKVRWGLLSTANINRRLIPAIRASQRGELVAVASRSKNTAAAY
                     ATRWDIPHAFGSYEGMLNSGMVDAVYISLPNHLHAEWSIRAMQAGVHVLCEKPFAISL
                     EEVDAMVAASHQYGRALAEAFMYRHHPQTKIAGEWAHSGRLGKICLVRSIFNFPLQNR
                     GNVRLVPEYGGGCLWDVGVYPLSFSQYILGRLPEWVIGAQWIGASGVDETFAGQMHYS
                     GDQMAQIASSFRSPQYTYAEIIGTEGQLILNRPFIGLGDPERQLMFYPVKGEAQEIPV
                     PEQELYVGEVEDMHAAILDNQPCYIKLEESRSHVQTALALYEAAQKGKSVSV"
     gene            1259..2047
                     /gene="pstB"
                     /locus_tag="AC16V7_12615"
     CDS             1259..2047
                     /gene="pstB"
                     /locus_tag="AC16V7_12615"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIR6258009.1"
                     /translation="MNILIPDGNISLEVRDLKVWYGTTPALKDLDLQIPSRKITAIIG
                     PSGCGKSTFIRCFNRMNDLIPGARVGGDVLYHGKNIYSPDIDPVEVRRSIGMVFQKPN
                     PFPKSIYDNIAYGPRISGYKGNMDDLVEKALRNAALWEEVKDKLKTSGLALSGGQQQR
                     LCIARAIAVEPEVILMDEPASALDPIATLKIEELMQQLKENYTIVIVTHNMQQAARSS
                     DYTAMMMLTEDMKSGTVIEFGRTTDIFTHPKDNRTEDYVTGRFG"
     gene            2078..2758
                     /gene="phoU"
                     /locus_tag="AC16V7_12620"
     CDS             2078..2758
                     /gene="phoU"
                     /locus_tag="AC16V7_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HIR6258010.1"
                     /translation="MIRELLDRKLQHLLDELLVLDGMVEQAIIESVDALKQQDLALAR
                     KVYDGDIKVNAKRFEIENECMITIATQQPIMAGDLRLLASILEVVGELERMGDYAKGI
                     AKICLMVGNEPHVKPLIDIPRMAEIAVSMLHRAVGAFINKDTETAKLIPKEDDMVDGL
                     YNQIYRELVAIMLTNPTTIDRANYLMWAAHNLERVADRVTNVCERTIYVTTGELKEID
                     VSDDESKV"
     gene            2762..3214
                     /locus_tag="AC16V7_12625"
     CDS             2762..3214
                     /locus_tag="AC16V7_12625"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HIR6258011.1"
                     /translation="MGKKTILFLCTGNSCRSQMAEAIVNARLGERWHAYSAGTEPTGY
                     VHPKALAALAEIGINHQGHTKSLDTFLNTRFDLVVTVCDSAAEECPYWPGADQTVHFD
                     FPDPAKANGSEEEIMTVFSKVRDEIAEKIVELLEQYLPSNIETHESVK"
     gene            3441..3800
                     /locus_tag="AC16V7_12630"
     CDS             3441..3800
                     /locus_tag="AC16V7_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258012.1"
                     /translation="MKIIAYIAAAILIFFGVLFIWGAFSPEGSVGWIPVGLISVGIGF
                     GLIWFASRKKPQVEASSQNVTLKIDLPGDVNLETLKCQSCGGTLTMDNIEMVAGAPVV
                     NCPYCKTTYQLTEEPKW"
     gene            3802..>4362
                     /locus_tag="AC16V7_12635"
     CDS             3802..>4362
                     /locus_tag="AC16V7_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258013.1"
                     /translation="MKKKIFPFLLLCLFALLPGSALAQTYYFSMDQETVHVFWNEDGT
                     TSIDYVFVFTNDNSASPIDFVDVGLPNSNFDKSTIFADVNGKEVTNISASDYQGGGDS
                     GVAVALGSYAIRPGQTGQVHVFVGTIRRMLRPDSDDKEYASGVFSPTWFGRQYVYGST
                     NLTVTYHFPPGVKPEEPRWHNSPSGWS"
BASE COUNT         1218 a         1010 c         1063 g         1071 t
ORIGIN      
        1 aagctcggtt aaccctttga gaatgacact gagaggcgaa aattttttcg gtatggctat
       61 aaaaaagggg gtggtcatgt tacactattc atgtctttga attgccttca cccaccgtga
      121 gaaatcagtt actatgccat ggggaaggct atttaatcct gaaattgaga ttacaggagg
      181 aacgatgtcg aaagttcgct ggggattact atcgactgcc aatatcaatc gacgcttgat
      241 ccctgccatc cgagcctcac agcgaggtga gctagtagct gttgccagcc gttcgaagaa
      301 cactgcagca gcctacgcta cacggtggga cattccccac gcatttggaa gctatgaagg
      361 catgctcaac tccggcatgg tcgatgcggt ctatatcagc ttaccaaacc atttgcatgc
      421 cgagtggagc atccgcgcaa tgcaagctgg agtacatgtg ctctgcgaga agccttttgc
      481 catctcattg gaggaggtgg atgccatggt cgccgccagc catcaatatg gacgggcgct
      541 ggctgaagct ttcatgtacc gccaccatcc gcagacaaag attgccggtg aatgggcgca
      601 cagcgggcgg ctgggaaaga tttgtctggt gcgcagcatt tttaacttcc ccctgcaaaa
      661 tagagggaac gtgcggctgg tgccagagta tggaggtgga tgcctgtggg atgtaggagt
      721 ttatccgctg agtttctccc aatatatact cgggcgtctt ccagagtggg taataggggc
      781 acaatggata ggtgcaagtg gtgtagacga aacctttgcc ggccagatgc attattctgg
      841 tgaccaaatg gctcaaatcg catcgtcttt tcgttcccct caatatacgt acgctgaaat
      901 catcggcact gagggtcaat tgatactaaa tcgacccttt atcggtcttg gagatccaga
      961 aaggcagtta atgttttatc cagtaaaagg cgaagcccaa gaaatccctg tcccagaaca
     1021 ggaattatat gtcggcgaag tagaggatat gcacgctgcc atcttggata atcaaccatg
     1081 ttatattaaa cttgaggaat cacgcagcca cgttcagact gccttggctc tctatgaggc
     1141 tgctcaaaaa ggaaaaagtg tttcagtata aaaactctgc gttgatgcct gtgtgttggt
     1201 gatatatatt atccgccatc attttgcgca atcgactagc ataccaggtg atatttacat
     1261 gaacatttta atacccgatg gaaacatctc cctggaggtt cgcgacctaa aagtatggta
     1321 tgggaccaca cccgcattaa aagatcttga ccttcaaata cctagccgaa aaattactgc
     1381 tattatcgga ccatctggct gcggtaagag cacttttatc cgctgcttca accggatgaa
     1441 tgacttgatt ccaggcgccc gggtgggtgg ggatgtactc taccatggta aaaatattta
     1501 ttcacccgat attgaccctg tcgaagtacg tcggagtatt ggaatggtat tccaaaaacc
     1561 caatcctttc cccaaatcaa tctatgataa tatcgcctat ggtccacgga tcagtggtta
     1621 caagggaaat atggatgatt tagtggaaaa agccttacgc aacgcagcgc tctgggaaga
     1681 agtaaaagac aaactcaaga catctggcct ggctctctcg ggcggtcagc aacagcgtct
     1741 ttgcatcgcc cgggcaattg ctgtcgagcc tgaggttatt ttgatggatg aaccggcatc
     1801 ggctttagat ccaattgcca ctttaaagat agaggagttg atgcagcaac tcaaagagaa
     1861 ttatacgatt gtgattgtca cccacaatat gcagcaagcc gcccgctctt ccgactatac
     1921 cgctatgatg atgctcaccg aagatatgaa atcggggacg gtgattgagt ttgggcggac
     1981 gactgatata ttcacccatc ctaaagacaa tcgtacagaa gattatgtaa ctgggcggtt
     2041 tggataggag agagaatcca gataaacggg gtagaacatg atcagagaac tgttagatag
     2101 aaaacttcag caccttctgg acgagcttct ggtcctcgat ggcatggtcg aacaggctat
     2161 catagagtct gtcgatgcat tgaaacaaca agacctggct ctagccagaa aagtatacga
     2221 tggcgacatc aaggtaaacg caaaacgttt tgaaattgag aatgagtgca tgattaccat
     2281 cgccacgcag cagcccatta tggctggtga tcttcgtttg ctggcttcaa ttctggaggt
     2341 cgtcggagag ctcgaacgta tgggcgatta tgcaaaaggc attgctaaaa tctgtttgat
     2401 ggtgggcaat gagccccatg taaaaccgct catcgatatc ccgcgcatgg ccgaaattgc
     2461 agtcagcatg ctgcatcgtg ctgtgggtgc gtttatcaat aaagacacag agaccgccaa
     2521 gctcattcca aaagaggatg atatggttga tggtctctac aaccagatct atcgagaact
     2581 ggtagccatt atgttgacca acccaaccac catagaccgc gctaattacc tgatgtgggc
     2641 cgcgcacaac ctggagcgag tagcagaccg cgtgaccaat gtatgcgaac gtacgatcta
     2701 cgtaaccaca ggcgaactca aagaaatcga tgtatccgac gacgagagta aagtataaac
     2761 tttgggtaag aaaacaatcc tctttttatg cacaggtaat tcctgtcgct cccaaatggc
     2821 tgaggcaatc gtcaatgctc gcctgggcga acgctggcat gcttatagcg ccggcaccga
     2881 accaacggga tatgtacacc ctaaagccct ggctgccttg gctgagatcg gaattaatca
     2941 ccaagggcac acaaaaagcc tagatacttt cctcaacacg aggttcgatc tggtagtaac
     3001 cgtttgtgat tcagccgcag aagaatgccc atactggcca ggagcagacc aaactgtgca
     3061 ctttgatttt cccgatccag caaaggccaa tggcagcgag gaggagatca tgaccgtctt
     3121 tagcaaggtg cgtgatgaga tcgctgaaaa gatcgtcgag ctgctcgagc aatatctccc
     3181 atcaaatatt gagacgcatg agtcggttaa ataaattaag ctgaggcaaa acaatctcag
     3241 aataatcatc ttataaaccc ctaccaccat gtgggcaaag ccaggatacg cataatcaat
     3301 gaagaattac gcggattctg gcttttatta tccgagttgt gacaaaataa ggaaatttat
     3361 catttgcccg aattcaatta aattattaaa cttataggaa caatccacgt atataggaca
     3421 tagacaggag caaattttca atgaaaatta tcgcctatat cgccgcagcc atattaatct
     3481 tttttggagt tctttttatc tggggggctt tcagcccaga aggctctgtc ggttggatac
     3541 ctgtcgggct aattagcgta ggaattggct ttggcttgat ctggtttgcc agccgaaaaa
     3601 aacctcaggt cgaggcaagc tcccaaaatg tcaccctcaa gatcgatctg ccaggagatg
     3661 taaacctgga gaccctcaag tgccaatcct gcggcggcac gcttacaatg gataacattg
     3721 aaatggtagc cggtgctcca gtagtaaatt gcccttattg caagactacc tatcagctaa
     3781 ccgaggagcc gaagtggtaa catgaagaaa aaaatatttc ctttccttct tctatgcctg
     3841 tttgcccttt taccgggcag cgccttggca caaacctact acttcagcat ggaccaagag
     3901 accgtccatg ttttctggaa tgaagacggg acaacctcga tagattatgt attcgtcttc
     3961 accaatgata attctgcatc acccattgat ttcgttgatg ttggtttacc taactcaaat
     4021 tttgataaaa gcacaatctt cgccgatgtg aacggcaaag aagtgaccaa catctccgcc
     4081 agcgattacc agggtggagg ggatagcggc gtagcagtcg ccttaggcag ctacgcaatt
     4141 agaccgggac agactgggca agtgcatgtc tttgtgggta ctatccgccg tatgctcaga
     4201 ccagacagcg atgacaagga atacgcgagt ggcgtgttct caccaacctg gtttgggcgt
     4261 caatatgtct atggttcgac aaatttaacc gtcacctacc actttccgcc cggcgtgaaa
     4321 cccgaagaac cgcgctggca taactcacct agcgggtggt ct
//
LOCUS       DBNHOH010000726         1482 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1390920, whole genome shotgun sequence.
ACCESSION   DBNHOH010000726 DBNHOH010000000
VERSION     DBNHOH010000726.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1390920"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            733..>1482
                     /locus_tag="AC16V7_12640"
     CDS             733..>1482
                     /locus_tag="AC16V7_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HIR6258014.1"
                     /translation="MGPKGEDGRRMIRLILGEEKRQEVMDALQGALGNSATAKIVVMG
                     TEAVLPRLDTNGDKPITKDITSNITREELYSNIEKGARLNGTYLILVILSTIVVAIGL
                     LEDNVAVVIGAMVIAPLLGPNIALALATALGDTELILKSLRTGAVGLIVALGLSVVIG
                     KMWHLEFDSQELLARTDVGLDSVALALASGAAAVLSLSTGLPSVLVGVMVAVALLPPT
                     ATMGLMLGIGKFNLALGAGLLLAVNVVCVNLS"
BASE COUNT          357 a          332 c          421 g          372 t
ORIGIN      
        1 gtaggcatcg ccatcggatg gaacataaaa cctaaacagg aataagctga ggttctgttt
       61 gaggaggagt tgggtcaggc gcactgtaaa tgtatgacgg tttttatcct gtgcaaatgt
      121 gccgggagga agtgactgca taaaacggtc atggtggatc atatgctcca gataatattg
      181 aaaaccgatt tcacctcatt gtggaaaatg cgaacgaact cattctctga aagcatgttt
      241 tccggatcgg tatcgatctg gattttggga atctgtagtc cgagggtcag ggtgagaacc
      301 agtacggcgg caacaatggc ccaaggatgg ttgagggaaa aatttaggat tgagggtctg
      361 tgggagccca tcttaaccat ggttattagt agaccgtata gatttggatt tattccttta
      421 acgtcatgtg taaaagctca tgctgcgatt gaagtgtaca actttcccga ctggggtaca
      481 gaagaaaatt gcaagcccga ggcgtggaaa acctttgtgc gtgggtctcc aaaattgact
      541 ctttcaattt accgccatcg tccttacaat atttatattc aagtggtttg ggaatctaga
      601 ccgatatcac cattcctgca accggcataa acgactacgc ccattatgaa aattatagaa
      661 attgtggccg atgtcagcta ccaggacatc gtcaccacca tcgcagaaca acatgatgtt
      721 cgggattgct ggttgggacc gaaaggggag gacggacgcc gtatgattcg cctgatcctc
      781 ggcgaggaaa aacggcagga agtgatggac gctctccagg gtgctttggg taacagcgcc
      841 acagccaaga tcgtggttat gggcacggaa gcggtattgc cccggctgga taccaatggt
      901 gacaagccta ttacgaaaga tattaccagc aacatcactc gcgaagagct ctacagtaat
      961 attgaaaaag gtgcgcggtt gaatggcacc tatttgatcc tagtgatact gtcgaccatc
     1021 gttgtggcga tcggtttgct ggaggataac gtagccgtgg tcatcggtgc gatggtcatt
     1081 gcacctttat tggggcccaa catcgccctc gccctggcca ccgccttggg agatacggaa
     1141 ctcatcttga aatccctccg gacgggggca gtcgggctta tcgtggcgct gggactttcc
     1201 gtggtgattg gcaaaatgtg gcacctcgaa tttgatagcc aagaactgct ggcccgaacc
     1261 gatgtcggat tggactcggt tgccctagcg ctggcttccg gtgcggcggc agtgctttct
     1321 ttgtctaccg gtcttcccag cgtactggtg ggcgtcatgg tggcggtggc cctgcttcca
     1381 cccacggcta ccatgggact gatgctcggc atcggaaaat ttaatttggc attgggcgca
     1441 gggctgttgc tggcggtgaa tgtggtctgc gtcaacctgt ct
//
LOCUS       DBNHOH010000727         2213 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_108727, whole genome shotgun sequence.
ACCESSION   DBNHOH010000727 DBNHOH010000000
VERSION     DBNHOH010000727.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_108727"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="AC16V7_12645"
     CDS             <1..467
                     /locus_tag="AC16V7_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding enzyme"
                     /protein_id="HIR6258015.1"
                     /translation="SRWPGIWVHGDFALIDDDDMWYILGRSDDTIKVAGKRLGPAEIE
                     SILVSHPDVVEAAAIGVPDEIKGSEVVAFAVLSTDCDPCEELRQELYKMIVKALGKPF
                     APREVLFVSDLPKTRNAKVMRRMIRAAYLGEDPGDTSSLVNPEAVEEIRVAR"
     gene            480..962
                     /locus_tag="AC16V7_12650"
     CDS             480..962
                     /locus_tag="AC16V7_12650"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR6258016.1"
                     /translation="MSDKAFQDFYSEQSSHCYGCGRLNELGLQIKSYWDGDETVCVFQ
                     PLPYHTAIPGYVYGGLIASLIDCHSTGSAAAAAYRAAGRSMDTLPAFRFLTASLHVDY
                     LRPTPIDTFLEVRSSIKEIKGRKIVVSSMLFARGDLCAKGEVVAVQMPDHLIGGLPDS
                     "
     gene            1021..2172
                     /locus_tag="AC16V7_12655"
     CDS             1021..2172
                     /locus_tag="AC16V7_12655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6258017.1"
                     /translation="MDFALSEEQRMWRDAVHEFVSREVKPKARHVDETGEFNWDAVRK
                     MGPLGLLGLNIPEEFGGAGVDAVSAAIAIEELGWGCGSTALAIAAHNGLGCAPLALFG
                     SVDLKQRFLSSVASGDGNLAALALTEPGAGSDLKGGVSVRASLDGNQWVINGAKMWCT
                     NASIADFIVTLVRTDPKGGSASLSMILVPTDSPGLHLGPPEKKMGLKGSPTHAVTYED
                     VRVPSDYLIGELGKGLQQTMVVLDGGRVSIGALSVGLAQAAFEEAIRYAQERQTFGQP
                     IANYQAIQWMLSNAATEIQAARLMVYYAAWLKEQGKPYSKEAAMAKMFASEMAERVCR
                     DAIQIHGGYGYSREYPVERIYRDARLMTIGEGTSEIQRLVIARHIMQEQ"
BASE COUNT          523 a          512 c          640 g          538 t
ORIGIN      
        1 ggtctcgctg gccggggatc tgggttcacg gtgatttcgc tttgatagat gatgatgata
       61 tgtggtatat cctcggtcgc tcagatgaca cgattaaggt agcaggtaag cgcctaggac
      121 cagccgaaat cgaatcaatt ctggtcagtc acccagatgt agtggaggct gcggcaattg
      181 gcgttcctga cgagatcaag ggcagcgagg tggtggcctt cgctgtgctt tcaacagact
      241 gtgatccatg tgaagagctg cgccaagagt tatacaagat gatcgtaaaa gctctgggca
      301 agccctttgc tcctcgtgaa gtcttgttcg tcagcgacct gccaaagacg cgcaatgcta
      361 aagtgatgcg gcgcatgatc cgtgctgcct atttggggga ggacccgggg gacacttcat
      421 cgctggtcaa tccagaagct gtagaagaaa tccgcgtagc aaggtaaaac aaacactcga
      481 tgagcgataa agcctttcaa gatttctact cagaacaatc cagtcattgt tacggttgtg
      541 gtcgattgaa cgagctcggc ttacagatca agagctattg ggatggagac gaaacagtct
      601 gcgttttcca gccgctcccg taccataccg ccattccggg ttacgtctat ggaggactga
      661 tcgcctcgct gatcgattgt catagcacag gctcagctgc cgcagcagct taccgtgcag
      721 cgggtagatc gatggacacc ttgcctgcat ttcgattctt aaccgcctcc ctgcatgtcg
      781 attatttacg cccaactccc atagatacat ttcttgaggt acgttcttcg atcaaagaga
      841 tcaaggggcg caagatagtg gttagctcaa tgctatttgc gcgtggagat ttatgcgcca
      901 agggggaggt ggtagcggtt caaatgcctg atcatcttat tggtggcttg cctgattctt
      961 aacggcattg atttgtgtca gaatcagaat gaactataaa cataattcgg aggagccatc
     1021 atggattttg ctttaagcga ggaacagagg atgtggcgcg atgcggtcca cgaatttgta
     1081 agccgcgagg tgaagcccaa agctcgccat gtggatgaaa cgggggaatt caattgggat
     1141 gctgtccgta aaatgggtcc tctgggtttg ttgggtttaa atatccccga agagtttgga
     1201 ggagcgggtg tagacgcggt cagcgcagcc atcgctatcg aagaactggg ctgggggtgc
     1261 ggcagcactg ctttggctat cgccgctcat aacggattgg gttgtgcccc acttgcctta
     1321 ttcgggtccg ttgatcttaa acagcgcttt ttatcgtctg ttgccagtgg agatggcaat
     1381 ttggctgccc tggcgcttac cgaacctgga gccggctctg acctgaaagg tggggtgagc
     1441 gtgcgtgctt ctctggacgg caaccaatgg gtgatcaatg gagcaaagat gtggtgcacc
     1501 aatgccagca tcgcggattt cattgttacc ctagtgcgca ccgatccaaa aggcggttcg
     1561 gcttccctga gtatgatcct tgtccccaca gattcccctg ggcttcacct tggtcccccg
     1621 gaaaagaaaa tgggattaaa agggtcccca actcatgctg tcacctatga ggatgtgcgt
     1681 gtgccatccg attacctgat tggtgaatta gggaaagggt tgcagcagac catggttgtt
     1741 ctggatggag ggcgggtcag catcggagcc ttatcggttg gtttggctca agccgcattt
     1801 gaggaggcaa ttcgctatgc ccaagagcgc caaacctttg ggcaacccat cgcaaactac
     1861 caggcgatcc agtggatgct ttcgaacgct gcaaccgaga ttcaagctgc tcgcttgatg
     1921 gtgtattacg cggcatggct aaaagaacag ggaaagcctt acagcaagga agccgctatg
     1981 gcaaagatgt ttgccagcga gatggctgag cgcgtctgtc gtgatgccat ccaaattcat
     2041 gggggttatg gctacagccg tgaatatcct gtggaaagga tttaccggga tgctcgattg
     2101 atgactattg gggagggaac cagcgagatc caacgcctgg taattgccag acacatcatg
     2161 caagagcaat aaactaaggc tcagccgcga aggctgtatc ttggttgaga aat
//
LOCUS       DBNHOH010000728         5912 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1519406, whole genome shotgun sequence.
ACCESSION   DBNHOH010000728 DBNHOH010000000
VERSION     DBNHOH010000728.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5912)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1519406"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..3715
                     /gene="rpoB"
                     /locus_tag="AC16V7_12660"
     CDS             11..3715
                     /gene="rpoB"
                     /locus_tag="AC16V7_12660"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR6258018.1"
                     /translation="MTYWFDEPKHNIEECVERDLTYARPLYVSVLLAGPDVPEPIKQD
                     IFLGDFPEMTEKGTFIINGTERVVVSQLIRSPGVYFEAPIDRSTGRPLAMAKLIPDRG
                     AWMEFETRKTDYLTLRFNRKRIVPVTIFLRALAAVNDSLPDSPLKHGTDEELLELFGD
                     VDNNPDRMFIASTLSQEPEWDLSSGISIAEAALLEFFKRMRPGDPATLDNAREFLEEQ
                     LFDQRHYDLERVGRYKLNQKLELHEHVPITHRMISKWDIVRLVRRMIMINNIMESPDD
                     IDHLGNRRAKTVGELIQNKLRIGLRRMERVIKERMSIRDQDQLSPITLINIRPVVAAL
                     REFFGSSQLSQFMDQTNPLAELRHKRTLSALGPGGLRRERAGFDVRDVHHSHYGRICP
                     IETPEGPNIGLIGRLASFARVNEYGFIETPYRRVRRTLASNDPKIEGRFLREDLIHPK
                     KGTVIAKSGDRITPKIAKSVAKLDLPEIAIVPFVTNEVVYLSADVEDRYVIAQANAPL
                     NENKEFVRRRVSSRFHSTFIFSESTNIDYMDVAPHQIVGISAALIPFLEHDDANRALM
                     GSNMQAQAVPLLRPDVPQVSTGMEYDAARDSGQVVIAEEDGEVVSVTGRSIVIRSDDG
                     KLRTYTLRKYQRSNQSTCIDQRPAVVKGQIVQRNDVIADSSSTESGELALGQNVMVAF
                     LSWEGGNFEDAILISERLVQDDAFTSIHIEKYEVESRDTRLGPEEITRDIPNVGEEAI
                     KDLDDQGIIRIGAEVGPNDILVGKISPKGEKELTPEERLLRAIFGEKSRDVKDTSLRM
                     PHGERGKVVDVKVFTREDNTDLSPGVDMMVRVSVAQRRKITAGDKMAGRHGNKGVVSK
                     VVPAEDMPFLEDGTPVDIILNPLGVPGRMNIGQVLETHLGWAASRLGFRAISPVFDGA
                     REDEIEAELARAWMINQSWKETGEVAWEWLNQQEYDPITIMDDDEVRRLYLEQWLGER
                     GYDVYQLISDAKYARRSVLREWLRDNGFDPDQILYFGAVESQEDVNPKPGLDENAVNA
                     CLRLWLKGRGENTKKIADDQLRSKAEEVTLRDRIPSPVLGKQILRDGKTGEAYHQPVT
                     VGVMTMLKLHHLVEDKVHARSTGPYSLVSQQPLGGKAQFGGQRFGEMEVWALEAYGAA
                     YTLQEMLTVKSDDVQGRVKTYEAIVKGEPIEEPSIPAAFRVLVKELQSLGLAVEAVTE
                     AGEVIKFGKDEERSKPPRLSTGLLGLGEDF"
     gene            3908..4330
                     /gene="rpsL"
                     /locus_tag="AC16V7_12665"
     CDS             3908..4330
                     /gene="rpsL"
                     /locus_tag="AC16V7_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIR6258019.1"
                     /translation="MPTTNQLVRKGRKSKKVKSKAPALLYTFNSMKQRRLRQEKGAPQ
                     KRGVCTVVRTMTPKKPNSALRKIARVRLTNGIEVTAYIPGEGHTLQEHSVVLVRGGRV
                     KDLPGVRYHIVRGTLDTGGVNDRQQGRSKYGSKRPKTK"
     gene            4348..4818
                     /gene="rpsG"
                     /locus_tag="AC16V7_12670"
     CDS             4348..4818
                     /gene="rpsG"
                     /locus_tag="AC16V7_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIR6258020.1"
                     /translation="MSRRYKPEKFDITPDVRYNSVNVQSFINRVMRSGKKSVAATLIY
                     NAMDTIQERTKRSPLEVFDQAFKNVSPVMEVKPRRVGGATYQVPMEVSPQRRFALASR
                     WIIAAARDRSGKTFSEKLAAELIDAANNTGSAIRKREETHKMAEANRAFSHYRL"
     gene            4895..>5912
                     /locus_tag="AC16V7_12675"
     CDS             4895..>5912
                     /locus_tag="AC16V7_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR6258021.1"
                     /translation="MARKLQLERYRNIGIIAHIDAGKTTTTERVLFYTGKTHRIGSVD
                     DGTTVTDWMEQERERGITIVSAAVTAEWKGYQINIIDTPGHIDFTAEVQRSLRVLDGG
                     IVIFDAVQGVEPQSETVWRQADRYRVPRICFVNKMDRVGASYERTIKMVRERLGANPI
                     AVQLPIGVESAFRGAVDLITMQALIWEDNLGKEPQQSEIPVDLRPQAEEMRARLIEQV
                     AETDDELTMKYLEGLDISEDELKLALRRAVIAGVATPVFCGSSLRNKGVQPLLDAVVD
                     YLPSPADVPAVTGMHPRTEETHERPPKDDAPLSALVFKIVTDPYVGRLAYLRVYSGKL
                     SQGST"
BASE COUNT         1408 a         1480 c         1676 g         1348 t
ORIGIN      
        1 ggaatggggg ctgacttatt ggtttgatga acccaaacac aacatcgagg aatgcgttga
       61 gcgcgatctg acatatgctc gacctcttta tgtctctgtg ctgctggccg ggccagatgt
      121 tcccgagccg ataaagcaag acatcttctt gggcgacttc ccggagatga ccgagaaggg
      181 caccttcatt atcaacggca ctgagcgggt tgttgtctcc cagttgattc gttctccggg
      241 tgtctatttt gaggcaccca tcgatcgttc cactggtcgc ccattggcta tggcaaaact
      301 catcccagac cggggcgcat ggatggagtt cgagacgcgc aagacagatt atctgacatt
      361 gcgattcaac cgcaagcgca ttgtgccagt gacgatcttt ctgcgcgcct tggcagctgt
      421 caatgatagt ttgcccgatt caccgttgaa acacggcacg gatgaggagc tgttggagct
      481 ctttggcgac gtggataata atcccgatcg catgttcatc gcttcaaccc tctctcagga
      541 accagagtgg gacctctcta gcggtattag tatcgccgaa gcggctttgc tcgagttctt
      601 caagcgcatg cgtcctggtg acccggcgac tttagataac gcccgcgaat ttctggaaga
      661 gcagttgttc gaccagcgtc attatgatct ggagcgtgtc ggtcgctata agctgaacca
      721 gaagctggaa ctgcatgagc acgtgcccat tacccaccgc atgatatcga agtgggatat
      781 cgtgcgactg gtgcggcgaa tgatcatgat caataacatc atggagtcgc cggacgatat
      841 cgaccatctg ggcaatcgcc gcgccaaaac ggtgggcgaa ctgatccaga acaagctgcg
      901 cattgggtta cgccgaatgg aacgggtgat caaagaacgc atgtccattc gcgatcaaga
      961 tcagctctca ccgataacac tcatcaatat ccgtccggta gtcgcggctc tgcgcgaatt
     1021 cttcggctcc agccagctat cccaattcat ggatcagacc aacccgctgg cagagctgcg
     1081 ccataaacgt accctgtcgg ctttaggtcc cggcggtttg cgacgcgagc gagctggctt
     1141 tgatgtgcga gacgtacacc actcccacta tggtcgtatc tgcccgattg aaaccccaga
     1201 aggccccaac attggtttga ttggccgcct ggcgtcattc gctcgggtca atgagtatgg
     1261 gtttattgag acaccttaca ggcgggtgcg ccggacgctg gcttccaatg atccaaaaat
     1321 tgaagggcgt tttctgcgcg aagacttgat acacccaaag aagggcactg taattgcgaa
     1381 atcaggcgac cgtataaccc caaagatagc caagtctgtt gccaagttgg atctacctga
     1441 aatcgctatc gtaccgtttg tgaccaatga agtggtgtat ctgtctgccg atgtagaaga
     1501 tcgctatgta atcgcccaag cgaacgctcc cctgaatgag aacaaggagt ttgtccggcg
     1561 gcgggtgtct tctcgtttcc actcgacttt catcttttca gagtcgacca acatcgatta
     1621 catggatgtt gccccgcacc agatcgtcgg cattagcgct gcgctgatcc cattcttgga
     1681 gcatgacgac gccaatcgcg ctctcatggg ttcgaacatg caggcgcaag cggtgcctct
     1741 gttgcgccca gatgtgcctc aggtttccac cggcatggaa tacgacgctg ctcgagactc
     1801 tgggcaggtg gtcatcgctg aggaggacgg tgaggtcgtc tcggtaactg gacgctctat
     1861 cgttatccgc tccgacgatg gtaagctgcg cacttatact ctgcgcaaat accagcgatc
     1921 gaaccagagc acatgcatag atcaacgtcc ggcggtggtc aaaggccaga ttgtgcagcg
     1981 caacgatgtc atcgctgact catcctccac tgagagcggt gagctcgcgt tgggacagaa
     2041 cgtgatggtg gctttcctct cctgggaagg cgggaacttc gaggacgcca tcctgatctc
     2101 ggagcgcttg gtacaggatg acgctttcac ctccattcac atcgagaagt acgaggtcga
     2161 atcacgggac acgcgcctag gtccagaaga gatcacccgc gatattccca atgtcggcga
     2221 agaggccatc aaagaccttg acgatcaagg cattatccgc attggggcgg aggtcggccc
     2281 gaatgacatt ttagtgggca agatctcacc caagggtgag aaggaactta ccccagaaga
     2341 gcgcctgctg cgtgccatct tcggcgaaaa gtcccgcgac gtaaaagata cttcgttacg
     2401 aatgccgcac ggcgagcgcg gtaaggtagt ggatgtgaag gtcttcaccc gcgaggacaa
     2461 caccgatcta tccccaggtg tggatatgat ggtgcgtgtc tccgttgcgc agcggcgcaa
     2521 gattaccgct ggggataaaa tggcaggacg ccacggtaac aaaggcgtag tgtccaaggt
     2581 cgtgccggca gaagatatgc cattcctcga ggatggtacc cctgtagata ttatcttgaa
     2641 tccgcttggt gtgcccgggc gtatgaatat cgggcaggtg ttggagactc atctggggtg
     2701 ggctgcaagc cgccttggtt tccgggccat ctcgcctgtc ttcgatggcg ctcgtgaaga
     2761 tgagattgaa gcggagttgg ctcgcgcctg gatgatcaat cagtcgtgga aggagactgg
     2821 cgaggtagca tgggagtggt tgaaccagca agagtatgat ccgatcacga tcatggatga
     2881 cgacgaggtg cgcaggttgt accttgaaca atggcttggc gagcgcggct atgatgtata
     2941 tcaattgatc tctgatgcca aatatgctcg ccgctctgtc cttcgcgaat ggctgcgcga
     3001 taacggtttt gaccctgacc agatcctata ttttggggcc gtggaaagcc aggaagatgt
     3061 caacccgaaa cctggtctag acgagaatgc cgtcaatgcc tgcctgcgcc tctggctcaa
     3121 agggaggggt gagaacacca agaaaatcgc cgatgatcag cttcgcagca aagcagagga
     3181 agtaacattg cgagaccgca tcccctcgcc tgtcctaggc aagcaaattc tacgcgatgg
     3241 aaaaaccggc gaggcgtacc accaaccggt aacggtgggt gtgatgacaa tgctcaagct
     3301 gcatcacctg gtggaagaca aagtccatgc tcgctccact ggtccataca gcttggtatc
     3361 tcaacagcct ctaggtggca aggcgcagtt cggcgggcag cgatttggtg agatggaggt
     3421 ttgggcgctt gaagcctatg gcgctgcgta tacgcttcag gagatgctca ccgttaaatc
     3481 cgacgatgtt caaggtcgtg tgaagactta tgaagctatc gtgaagggtg agcctattga
     3541 agaacccagc ataccggccg cattccgagt tctggttaaa gaactgcaga gtctggggct
     3601 ggcggtagaa gcggtgaccg aagccggaga ggtgattaaa ttcggtaagg atgaggagcg
     3661 aagcaagccg ccaaggcttt ctactggctt gctggggctg ggtgaggatt tttagataat
     3721 cgaataatgg ccttgacgct cactatcgcg catggtagaa tggttgttcg ttgcccatgg
     3781 ataaggcagg cggcaattat ccccgcctgg catgtttacg agtgaatttc agagaggcca
     3841 tcaagcaagc ggatggcctc tctgaatgga tagaagttgt ttagttggaa acttttcgga
     3901 ggcaaaggtg cctacaacaa atcaattagt acgcaaaggc cgcaagtcaa agaaggttaa
     3961 gagcaaggct cctgctttgt tgtatacatt taattcaatg aagcaacgtc gccttcgcca
     4021 ggaaaagggc gcgccacaga agcgcggtgt gtgtactgtg gtacgtacta tgacgccgaa
     4081 aaaaccaaac tctgccttgc gcaagattgc acgcgtgcgc ctgacaaatg gtatcgaagt
     4141 aaccgcctat atcccaggcg aaggtcatac gctccaggaa cactcagttg tcctggtacg
     4201 cggtggtcgt gtgaaggatc tgcccggcgt gcgttatcac attgtgcgcg gcaccctgga
     4261 tactggcggt gtgaatgatc gccagcaagg gcgctcgaag tatggttcca agcgacccaa
     4321 gacaaagtaa ggataggtag tgatattatg tctcgacgtt acaagcccga aaaatttgat
     4381 attactcctg acgtgcgcta taacagcgtt aatgtccagt ccttcattaa ccgcgtgatg
     4441 cggagtggta agaaaagcgt tgctgcaact ttgatctaca atgccatgga caccatccag
     4501 gagcgaacca aacgtagccc gttggaggta tttgaccagg cttttaaaaa tgtatctccg
     4561 gtaatggaag tcaagccgcg ccgagttggt ggggcgacct accaggtgcc gatggaggta
     4621 tctccgcagc gacgttttgc tttagcttca cgctggatta tcgcagctgc aagggatcgc
     4681 tcgggcaaga ctttctctga aaagttagcc gctgagctga tcgacgccgc caataacact
     4741 ggttcggcta tccgtaaacg cgaagagacc cataaaatgg cggaagccaa tcgcgccttt
     4801 tcgcattatc gtttatagta ttttactttc agcggtcagc ctcatctgat gaatgaccgc
     4861 tgaatcttga tgactgattg taattatagt aactatggca cgaaagcttc agttggaacg
     4921 ataccgaaat attgggatca ttgctcatat cgatgcgggc aagaccacca cgactgagcg
     4981 cgtcttgttc tatacaggca agacacatcg cattggctca gtggatgatg gcaccaccgt
     5041 taccgattgg atggagcaag agcgcgagcg tggtatcact attgtctcgg ctgcagtcac
     5101 ggcagagtgg aagggttatc aaataaatat tattgatacc cctggtcata ttgacttcac
     5161 cgccgaagtg cagcgttctc tgcgtgtctt ggacggaggc attgtcatct tcgatgccgt
     5221 tcagggggtc gagccgcaaa gcgagacagt gtggcgccag gctgatcgct accgcgtgcc
     5281 acgaatttgc ttcgtaaata aaatggaccg cgtgggcgca tcttacgagc gcacgattaa
     5341 gatggtccgc gagcgtttgg gcgctaatcc tattgctgtg cagttgccga ttggcgtcga
     5401 atctgctttt cggggcgcag tggacctgat aaccatgcag gcattaatct gggaagataa
     5461 cctgggcaag gagccgcagc aatctgaaat tcccgtcgat ttgcgtcccc aagctgaaga
     5521 aatgcgtgcg cgcctcattg agcaggtcgc cgagactgac gatgaactca ccatgaaata
     5581 tctggaaggt ctcgatatca gtgaagatga gctcaaacta gctttgcgta gagcggtgat
     5641 cgccggtgtt gccacaccgg tattttgcgg gagttcgttg cgcaacaaag gtgtccaacc
     5701 tctcctagat gcggtggtag attacctgcc ctcaccggca gacgtgcctg cggtaacagg
     5761 catgcatcct cgcacagagg aaacgcatga acggccaccc aaagacgacg ctccgctgag
     5821 cgcattggtc tttaaaattg tgacagatcc ctatgtgggt cgattggcct atctgcgtgt
     5881 ttactctggc aagctcagtc agggttcaac cg
//
LOCUS       DBNHOH010000729         1498 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1391078, whole genome shotgun sequence.
ACCESSION   DBNHOH010000729 DBNHOH010000000
VERSION     DBNHOH010000729.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1391078"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="AC16V7_12680"
     CDS             <1..662
                     /locus_tag="AC16V7_12680"
                     /inference="COORDINATES: protein motif:HMM:NF025109.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6258022.1"
                     /translation="SPGLYDSLSADYNLQIYANLYEVKDVRGQVEKYLRMLGLWDRRQ
                     DEVGTFSKGMQQKLAIASAILHEPRILFLDEPTAGLDPEASHLVREFIEELRKQGRTI
                     FMCTHNLDEADRLCDKIGVFKTRLLTLDTPTNLRTQLFGRKVVFHLSHVEETYLALLR
                     SMPFVKEARAVDNKLVATLDDPENQNPEIVRNLVEAGAEIRFVGELRHSLEDVYLQLV
                     K"
     gene            659..1447
                     /locus_tag="AC16V7_12685"
     CDS             659..1447
                     /locus_tag="AC16V7_12685"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR6258023.1"
                     /translation="MNKIKTIILKEWAQVFKNRLVLSTLVFMPLLFTAIPLMMLYATR
                     GTGAQELSSDMPPQFTSMCSQGLTAGECFQVYIVNQFMMLFMMIPLIIPVNIAAYSIV
                     GEKTTRSLEPLLATPITTAELLLGKNLAAAIPAVVATWMGFLLYVLGAWIIVQSSRVV
                     AALLDPMWWLAVFVAGPLLAVLSVNFALMVSSRVNEPRVAEQLSAIVILPLLALFIGQ
                     IAGLFLINRQLVLLFTIILALIDALMIYVAIRLFQREVILTRWK"
BASE COUNT          363 a          385 c          367 g          383 t
ORIGIN      
        1 aatcgcctgg tttgtacgac agcctgagcg ccgattacaa tcttcagatt tatgcaaatc
       61 tgtatgaagt gaaggatgtg cgcggtcagg tggagaaata cctgcggatg ctaggcctgt
      121 gggacagacg ccaggatgag gtgggtacat tctcgaaagg catgcagcag aaattggcaa
      181 ttgccagtgc catactccac gaaccgcgta tattgtttct ggatgaaccc acagccgggc
      241 tcgaccctga agcctcacac ttggtgcgcg agtttattga agagctgcgc aaacaaggcc
      301 gcacgatctt catgtgcacg cacaacctcg acgaagccga tcgtctgtgt gacaaaattg
      361 gcgtatttaa aacccgatta ttaaccctgg atacacccac caacctgcgc acacagctgt
      421 tcgggcgcaa agtagttttc catctcagtc acgtcgaaga aacctatctc gcattgctcc
      481 gatcgatgcc cttcgtcaaa gaagccaggg cagtcgacaa taaactggtg gcaaccttgg
      541 acgatcccga gaaccaaaat ccggaaatcg ttcgtaacct ggtagaggct ggcgcagaga
      601 ttcgctttgt tggcgagctg cgccactctt tggaagatgt gtacctacaa cttgtcaaat
      661 gaacaaaatt aagaccatca tcctcaagga atgggcccag gtgttcaaaa accgcctggt
      721 gctctcaacc ctggtattta tgccgctgct cttcacagcg attccgttga tgatgcttta
      781 cgcaacgcgc ggaacgggcg cacaggaact aagcagcgat atgccgcccc aatttacatc
      841 catgtgctca caaggcctta ctgctggtga atgctttcag gtctacatag tcaaccagtt
      901 catgatgcta tttatgatga tccccttgat cattccggtc aatatcgcgg cctattctat
      961 tgtgggggaa aaaaccacac gcagcctgga gccactcttg gctacaccca tcacaaccgc
     1021 tgagttgctc ttgggtaaaa acctcgccgc agctatccct gctgtggtag cgacatggat
     1081 gggctttctg ctctatgtgc tcggcgcatg gatcatcgtg cagtcatccc gtgttgtggc
     1141 tgcattgttg gacccgatgt ggtggctggc agtattcgtg gcagggccat tgctagccgt
     1201 cttatccgtg aatttcgccc tgatggtctc ttctagggtt aacgagccgc gcgtggcaga
     1261 acaactttca gcgattgtga ttctgccact cctggcactt ttcatcgggc aaattgccgg
     1321 gcttttctta atcaaccgcc aactcgtttt actttttaca ataattctgg cacttattga
     1381 tgcgctaatg atctatgtgg ctattcgatt attccagcgc gaggtgatat tgacccgctg
     1441 gaaatgacac ctcataatcg atgaagaaac taaccaagtt atgtttaata ttgatggt
//
LOCUS       DBNHOH010000730         8124 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_813997, whole genome shotgun sequence.
ACCESSION   DBNHOH010000730 DBNHOH010000000
VERSION     DBNHOH010000730.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8124)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8124)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8124
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_813997"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="AC16V7_12690"
     CDS             <1..373
                     /locus_tag="AC16V7_12690"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR6258024.1"
                     /translation="GCIRYRLGDFQYSQSEMWRHVWRLIPSLLKNRIQHGRYLDIFIT
                     HAPPRGIHDQDDLPHHGVDAFRWLIQTFHPAYHFHGHIHVYRPDAPVESLVGMTKVIN
                     TYGFRETLITNQRQIKTGLRA"
     gene            complement(340..2067)
                     /locus_tag="AC16V7_12695"
     CDS             complement(340..2067)
                     /locus_tag="AC16V7_12695"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR6258025.1"
                     /translation="MNYDPNQRNLDTYRYITSLQDFRRARSQAKLKDLVARVTGESTE
                     LLSYEEVRQKLRAYASSNRGLQDIPLDAIVGSVGRYNDFTRDFLPRQDFTQDRWARVK
                     AASEASRGLPPIDVYKIGEAYFVQDGNHRVSIARQYGSKTIEANVTEVLVRVPLDPDT
                     QPDDLILKAEYAGFLERTHLDELRPEADLSVTIPGQYPVLEEHIAVHRHYMGQDQQRF
                     IPNDEAVADWYDSIYLPAIRAIQEGGILRNFPDRTETDLYLWIAEHRAALENEWGVEI
                     KTEVAALDLLKHEKPDADSVFARIGRRILESGPPPGKWRQEAQVIHRDEWLFTDILVP
                     ISGAPHSWLALEQAMVIAHREAARLHGLHVLSEERNGVTEELQGEFVRRCAEQGLEGH
                     LLVTSGEVAQQISIQARLTDLVVTTLAYPPPPKFLARLDSGFHELIQRCPRPVLAVPQ
                     VVTPLNHALLAYDGSPKADEALFVAAYLAGCWKIPLTVATVFDEKRVAPETLLRAKIY
                     LEDHDVQANYSPETGDVAEAIINASDAHQADLIIMGGYGFNPVMEIILGSAVDQVLRQ
                     ARKPVLICR"
     gene            2251..3063
                     /locus_tag="AC16V7_12700"
     CDS             2251..3063
                     /locus_tag="AC16V7_12700"
                     /inference="COORDINATES: protein motif:HMM:NF046770.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaI-LiaF-like domain-containing protein"
                     /protein_id="HIR6258026.1"
                     /translation="MKRGTLFWGFVLIILGLALLLDNLGILGNIDIWNLLWPLFLIAL
                     GAWILFGRVVRRAPTTEHVSIPLEGAQSARVRVGHGAGRLDVYAGSGLGNLVEGDFTG
                     GLDYRSQLSGDVLEVKMRPPETYFVIVPKYFPFDWSPGLTLDWSFGLTADIPLSLDFE
                     TGASESRLNLRDLRLTELRLKTGASSTSIDLPANAGQTRATIESGAASVNIAIPEGVA
                     ARIRTQGGLSTFEVDTNRFPQVGGVYQSPDYDTATNRIDMDIQMGVGSVKIY"
     gene            3076..4629
                     /locus_tag="AC16V7_12705"
     CDS             3076..4629
                     /locus_tag="AC16V7_12705"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HIR6258027.1"
                     /translation="MKPRQRRMAEGILFTDQYQLTMAQLYYRMGLHEKRVQFDHYFRD
                     YPQYNSHKAGYCISAGLEWLIDWMSEARFGEQEIEYLHSHQSSAGGRLFAEDFLNWLQ
                     QNGNFDGISLQAIPEGRVIHPNEPITVVQGPLAMAQILETSLLNHINYQILIATKAAR
                     IREIGRGQVMLEFGLRRGHGTGANAGARAALIGGADYSSNVGISAIMGYPPKGTHAHS
                     MVQLFIALGMSELDAFQAYADVYPDDCLLLVDTINTLESGVPNAIKVFDNLRKKGHKP
                     VGIRLDSGDLAYLSIQAAKMLDKAGFNDASIVLSNNLDELIIWQIVTQIEEEAPRYGV
                     IPDQLIRRLVYGVGTRLITSWGEPALGGVYKLVAVCDEGVWKPAIKISESISKTPNPG
                     HKQVWRLYDQRGNATADLLSLEDENLKDAKQIVLRHPTDHAKYRVLARDQISEIESLL
                     VEVLKDGKLVVDLPSIEQMRQQRLADVERLDAGVRRIMYPHIYHVSLTPELWDLKQKL
                     IKSTMSGIH"
     gene            4711..5349
                     /locus_tag="AC16V7_12710"
     CDS             4711..5349
                     /locus_tag="AC16V7_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258028.1"
                     /translation="MVKWLESRVLWGSLLILAGVVFLLQNLFHIDIGAFFWAVLLAMA
                     GAVFITVFISNRQLWWALIPGFTLLGVSATVFISNYLPRLEGVLGGFFVLGGIGLAFV
                     VIYLNDRRFWWALIPAGVLFTVAIISVVEGLFGGLETGGIFFIGLGLTFAAVAIFPAP
                     QGKMRWAWYPAAVLTVMGLLIAAAAENLINYVWPVALILGGLVLIYRNIASR"
     gene            5359..5826
                     /locus_tag="AC16V7_12715"
     CDS             5359..5826
                     /locus_tag="AC16V7_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIR6258029.1"
                     /translation="MENKLYRSRTDAMLGGVCGGLAHYLRIDSTLVRLFFILIALGGS
                     GIGVFVYLLLWIIIPLEGQKRDVTLQDTVRSGSEEIAERTRQMGDDLRHLVRNPNPQA
                     GLLIGAALIILGAIFLIQNLDLPWLYWLDFDVIWPALLIVGGIALLMRHWRGE"
     gene            5828..6898
                     /locus_tag="AC16V7_12720"
     CDS             5828..6898
                     /locus_tag="AC16V7_12720"
                     /inference="COORDINATES: protein motif:HMM:NF046770.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF transmembrane domain-containing protein"
                     /protein_id="HIR6258030.1"
                     /translation="MSERKETPHRRPRSLFGPLLLIALGIVFLLNNLGILRGDGWNTV
                     VQLWPLILIVIGLDSLYQRQGLVGAIFIIGLGTVFLLSNFNLLNVNAWQLVLRLWPLL
                     LVAIGLDLIVGRRSLWASLAGLAVLLAILAGVLWLQGVRLGAGQVLSGQEVRQSFQGA
                     TRAEIILQSSAGDVYIHSLPGSNELIAGQVASGKRRRIFEEISMQGEQANYRLREDEN
                     FGYFWGRAGQRTWDLGLTPTIPLEIHYSQGAGVSNLDLSELQVYALRVNMGVGQTTVT
                     LPAKGEFDGRIEGAVGQLIIIVPKGMELRVRSNLALANIITPAAYRKRDRVYTSPGYE
                     TAANRIDLEVGMAVGNVMIREK"
     gene            complement(6940..>8124)
                     /locus_tag="AC16V7_12725"
     CDS             complement(6940..>8124)
                     /locus_tag="AC16V7_12725"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HIR6258031.1"
                     /translation="SRLFFSSLDEFLTEQREGRQVGAYSHQVRIQPATRTLSTWASVE
                     AAWDEAEHTLSPMLDVLAKLLQGLSEILPALPEGDEELFSALSNLYHRFTELHDNMEG
                     MVFKPDQGLIYWAEANTNSSSVALNAAPLEIGPLMQRYLWHEKSAVILTSATLTAAGE
                     FTYLRHRLYAEEADELSVGSPFDYENAALLFVANDIPEPGDRQGHQRGVESGLIGLCR
                     AIGGRTLVLFTSYDQLRRTSNAISPILAKNDILVYEQGEGASPHSLLESFRTSERAVL
                     LGTRAFWEGVDIPGEALSVLVIVKLPFDVPSDPIIAARSETFEDPFYEYSLPEAILRF
                     RQGFGRLIRTQSDRGVVAILDRRILTKRYGRLFIDSLPECTSRVGPLGELPRLAKQWL
                     NL"
BASE COUNT         1884 a         1916 c         2257 g         2067 t
ORIGIN      
        1 aggctgcatt cgttacaggc taggtgattt tcaatattcc caatctgaga tgtggagaca
       61 tgtatggcga cttattccct cattgctcaa aaaccgcatc caacatgggc gctacctgga
      121 tattttcatc acccacgcgc cacctcgcgg catccacgat caggacgact tgcctcacca
      181 tggcgtcgat gccttccgct ggctgataca gacttttcat ccagcctacc atttccatgg
      241 tcacattcac gtctaccggc cggatgcgcc agttgaaagc ttggtgggta tgacaaaggt
      301 gatcaatacc tacggttttc gcgagacctt aattacaaat cagcgacaaa ttaagacggg
      361 tttgcgcgcc tgacgtagca cctggtccac agcacttcct aggatgatct ccattacagg
      421 attaaacccg tagccaccca taatgatcaa atcagcctga tgagcgtctg atgcattgat
      481 gattgcttct gctacgtcac cggtctctgg ggaatagttg gcctgaacat catgatcttc
      541 caaatagatc ttggcgcgca gcagcgtttc gggggcaacg cgtttttcat caaatacggt
      601 ggctaccgtg agggggattt tccagcagcc tgctaaatag gcggcaacaa ataaagcctc
      661 atccgctttg gggctgccat cgtaagccag cagggcatga tttagcggag tgacaacttg
      721 agggactgcc aatactggtc tgggacaacg ttgaatgagt tcgtgaaagc cagaatctag
      781 ccgggcgagg aatttgggcg gaggcggata tgctaaggtg gtaactacaa gatcggtcaa
      841 tctggcttga atggatatct gttgggcgac ctcaccggat gtaacaagta gatgcccttc
      901 caagccttgc tcggcgcaac gccgcacaaa ttcaccctgt aactcctccg taactccatt
      961 tctctcttcg gagagtacgt gtagcccatg caatcgggca gcttcgcggt gggcgatcac
     1021 catagcctgc tccagggcaa gccagctgtg gggagcgcca cttattggaa ccaggatgtc
     1081 tgtgaacaac cactcgtcgc gatgaataac ctgtgcttct tgacgccatt tacctggggg
     1141 agggccgctc tcaagtattc ttctgccaat tcgtgcgaag accgaatcag cgtctggttt
     1201 ctcatgcttg agcaagtcta gcgcggctac ttcggtttta atctccactc cccattcatt
     1261 ctccaaagcc gcacgatgtt cggcgatcca taaatatagg tcggtttccg tgcggtcggg
     1321 aaaatttcgc aggataccac cttcttgaat tgctcttata gcaggcaaat agattgaatc
     1381 gtaccagtca gctactgcct catcgtttgg gataaatcgt tgttgatcct gccccatata
     1441 atgacggtga actgcgatat gctcctccag aacggggtat tgcccgggta tcgtcacact
     1501 taaatccgcc tcaggtcgca attcatctag gtgggtgcgc tctaagaatc cagcatattc
     1561 tgccttcaaa atcaggtcat ccggctgagt atctggatca agagggactc tcacaagaac
     1621 ctcagtgacg ttggcctcga tggttttaga tccgtactgg cgagcaatag acactcggtg
     1681 gttgccgtct tggacgaaat aggcttcacc aatcttataa acgtcgatgg gcggcaatcc
     1741 gcgggaggcc tcggaagccg ccttgaccct tgcccaacga tcctgggtga agtcctgacg
     1801 agggagaaaa tccctcgtaa agtcattgta acgacctaca cttcctacta tggcatccaa
     1861 ggggatatcc tgaaggccgc ggttcgagct ggcatacgcc ctgagcttct gccgtacttc
     1921 ttcgtaggac agtagttcag tggattcacc cgtgacgcgc gcaaccaaat ccttaagttt
     1981 tgcctggcta cgggcacgac ggaaatcctg taaggaggtg atatagcggt aggtatctag
     2041 gtttcgttga tttggatcgt aattcacgct aatattttaa tacgaaacca gccgatggag
     2101 gtaaattaca tctttatcgg ggtattcagg tggattttat taacaatttc cctgctaaat
     2161 aataggtgta gaatacagat atagattttg tgtgtctaac acgcgacagg atccaccttt
     2221 tctacgtatt aatacaagga gtaaacgaac atgaaaagag gcactctttt ttggggattc
     2281 gtattgatca tcctaggttt agctttgctt ttagacaatc taggtatatt ggggaacatc
     2341 gatatctgga atttgctctg gccgttattc ctgatcgccc tgggggcttg gattttgttc
     2401 ggtcgggtgg tcaggcgcgc tccaacaacc gaacacgtca gcatacctct cgaaggtgca
     2461 cagagcgcac gggtacgcgt ggggcacggg gctggaaggc tagatgtata cgctgggtca
     2521 gggttgggta atctggtgga gggtgacttc accggaggac tggattatcg cagccagctc
     2581 tcaggagatg ttttggaggt caagatgcga ccgccagaaa cctatttcgt gatcgttcca
     2641 aagtatttcc cgtttgattg gtcgcccggt ctaacattgg actggtcgtt tggacttacg
     2701 gctgatattc ctctgtcgct ggatttcgag accggggcaa gtgaaagccg attgaattta
     2761 cgcgatctcc gcctgacgga gctacgcctc aagactgggg ctagctcgac atcgatcgat
     2821 cttcctgcca atgcagggca aacgcgcgcc actatcgaaa gcggagctgc ctcggtgaat
     2881 atcgcaatcc ccgaaggcgt agccgcgcgc atccgtacac aaggtgggct ttccacattc
     2941 gaagttgaca ccaaccgttt cccccaagtg ggcggcgtat atcaatctcc agattatgat
     3001 accgccacca ataggatcga tatggatatc cagatggggg ttggatctgt caagatttat
     3061 taatcaggtt ttgctatgaa accaagacaa aggcgtatgg ctgaaggtat ccttttcaca
     3121 gaccagtatc agctaaccat ggcgcagcta tattaccgta tggggctgca cgagaagcgt
     3181 gtccagtttg accactactt ccgagattat ccccaatata actcgcacaa agcgggttac
     3241 tgcatcagtg ccgggctgga gtggttaata gattggatga gcgaggcgcg atttggcgag
     3301 caagagattg aatacttgca tagccatcaa agtagcgcag gcgggcgcct gtttgcagaa
     3361 gattttctga attggctaca gcaaaatggc aattttgatg gcatcagctt gcaggcgata
     3421 ccagaagggc gcgtgataca cccaaatgag ccgatcacgg ttgtccaggg tccgcttgcg
     3481 atggctcaga ttttggagac ttcgctgctc aatcatataa attaccaaat cctgatcgcc
     3541 accaaagctg ctcgtatccg ggagattgga cgggggcagg ttatgctgga atttggcctg
     3601 cgtcgtgggc atgggaccgg agcgaacgcc ggggcacgcg ccgccttgat tggcggtgct
     3661 gactattcat ctaatgtggg gatttcggcg atcatgggct acccgccaaa gggtacccat
     3721 gcgcacagca tggtgcaact cttcatcgcg ttgggtatga gcgaactgga tgcatttcag
     3781 gcttacgcag acgtctaccc cgatgattgt ttgcttttgg tagataccat caacacgctg
     3841 gagagcggtg tacccaatgc aattaaggtc tttgataatc tgcgcaaaaa ggggcataag
     3901 ccggtaggca tccgcctgga ttcgggtgat ttggcttacc tctccatcca ggctgccaag
     3961 atgctggata aggccggctt taacgatgca tccatcgtgc tctcgaataa tctggacgag
     4021 ttgatcattt ggcagatcgt cacacagatt gaagaggagg cgccccgtta tggggttatc
     4081 cctgaccagt tgatccgcag gctggtgtat ggggtgggca cgcgcttgat cacctcttgg
     4141 ggcgaaccag ccttgggtgg ggtttataaa ctggtagctg tttgcgatga aggggtgtgg
     4201 aagccggcta tcaagatttc cgaatcgatc tcgaaaacgc caaacccagg tcataaacag
     4261 gtctggcggc tgtatgacca gcgtggcaac gctaccgccg atctgctcag cttggaggat
     4321 gaaaatctga aggatgcaaa acagattgtg ttgcgccacc ctacggatca tgccaagtac
     4381 cgggtgctgg cgcgcgatca gatctccgag attgaatcgt tgctggtcga ggtcttgaaa
     4441 gatggaaaac tggtcgttga cttgccaagc atcgagcaaa tgcgccaaca gcgcttagcg
     4501 gatgttgagc gcctggatgc cggtgtccgc cgcatcatgt atccccacat ctatcacgtc
     4561 tcactaaccc cggaattgtg ggatctaaag caaaaattga tcaaatcgac gatgtctggc
     4621 attcattagt gcaagtagat gcgtagttga agaaggtctc tccaactgta tatcttctca
     4681 ataaatttcg taggatcgag gaggatgatc atggtcaaat ggctggaatc tcgtgtcttg
     4741 tggggatcat tattgatcct ggccggagta gttttcttac tgcaaaattt attccatatt
     4801 gatattggcg catttttctg ggcagtattg cttgccatgg cgggcgcagt attcattact
     4861 gtctttatta gcaaccgtca actgtggtgg gctttgatac caggttttac tcttttaggc
     4921 gtttctgcca ccgttttcat aagcaactat ttacccagat tagagggtgt cctgggtggt
     4981 ttctttgtct taggtggaat tggtttggct tttgtcgtaa tctacctgaa cgaccggcgt
     5041 ttctggtggg cattgatccc ggcgggggtg ttatttaccg tggctataat cagcgtggtc
     5101 gaaggtttgt ttggaggatt ggaaacaggc gggatatttt tcatcgggct ggggttgact
     5161 tttgccgcgg ttgctatttt tcctgctccg caagggaaga tgaggtgggc atggtaccca
     5221 gccgcagtgc tgacagtgat gggtttgctg atcgcagccg cggctgagaa cctgattaat
     5281 tacgtttggc cggtagcttt gatcttgggg ggtcttgtcc tgatatatcg caatatcgca
     5341 tctcggtgaa ggagtgaaat ggagaacaaa ctatatcgca gcagaacgga tgcaatgctt
     5401 ggtggtgttt gtggaggcct ggcacactac ctgcgcatcg attcaaccct ggtgcgcctg
     5461 ttttttatcc tgattgcgtt gggaggcagt gggatcgggg tttttgtcta cctgttactg
     5521 tggattatca ttcctttgga aggtcaaaag agggatgtga ctttacagga tacagttcgc
     5581 tctggcagcg aagagattgc cgagcgaaca cgccagatgg gcgatgatct gcgccatctt
     5641 gtgcgtaatc ccaatccaca ggctggcttg cttattggcg cagcattaat tattttgggt
     5701 gcgatcttcc ttatccaaaa tcttgatctg ccctggttgt attggttgga ctttgatgtg
     5761 atttggccag ccttgttgat tgttggaggc attgcactgc tcatgcgcca ttggagagga
     5821 gaataagatg agcgaaagaa aagaaacgcc gcaccgcagg ccaagaagcc tgtttggtcc
     5881 attgttatta atcgccttgg ggattgtatt cttgctcaat aacttgggca tactccgggg
     5941 tgatggatgg aatacagtag tgcaattatg gccgctgata ttgattgtca ttggcttaga
     6001 cagcttatat cagcgacagg ggctggtcgg tgcgatattt atcatcggct tgggcacggt
     6061 cttcctgctg tctaacttta atctgctcaa tgttaatgcc tggcaacttg ttctgcggct
     6121 ctggcctttg ctattagttg cgattggctt ggatctgatc gtgggcaggc gctcgctgtg
     6181 ggcatctcta gcgggtttgg ctgtactgct ggccattctg gcgggtgtct tatggctcca
     6241 gggtgtgcgg ctgggagcag gacaggtttt gtcgggtcaa gaagtgcgcc agtcttttca
     6301 gggagcaaca cgcgccgaga tcatccttca aagtagcgca ggcgatgtct atattcattc
     6361 tttacctggc tcgaatgagc tgatagctgg ccaggtggca tctggaaaac gcagacggat
     6421 atttgaagag atatctatgc aaggggagca agccaattac cgtttgcgcg aagatgagaa
     6481 ttttggatat ttttggggac gcgctgggca gcgtacctgg gatctgggct tgactccgac
     6541 aattcccctc gaaattcact acagccaggg ggcgggggta tccaacctgg acttgagcga
     6601 gttacaagtc tacgccttaa gggtcaacat gggtgtaggc cagaccactg tcaccttgcc
     6661 agccaaggga gagttcgatg gcaggattga aggagctgtc gggcagctga ttatcattgt
     6721 gcctaaagga atggaattga gagtgcgctc caatctggct ctggctaata taatcacccc
     6781 ggctgcttac cgaaagaggg acagagtata tacctcccct ggatatgaga cagcggcgaa
     6841 tcgcatcgat cttgaagtcg gaatggctgt cgggaacgtg atgatccggg agaagtaata
     6901 acctgaaatg aattagagaa gcaacaaaac tcgtcttctc tataaattca accactgttt
     6961 tgccagcctg ggcaattctc ccagcgggcc aacacgcgag gtgcattctg gcagggaatc
     7021 tatgaaaagg cgcccatagc gtttggtcag gatgcgccta tccagtatgg caaccacacc
     7081 acggtcactc tgggtgcgga tcaggcgccc aaaaccctgg cggaagcgca agatcgcctc
     7141 tggcaaggaa tattcgtaga atggatcttc aaaggtctcc gagcgggcgg caataatcgg
     7201 atcggaaggc acgtcaaagg gaagcttgac aatcactagt acagacaggg cttctccggg
     7261 gatatccaca ccctcccaaa aggcacgcgt acccaataat acggcacgct cgctagtgcg
     7321 gaaactctcg agcaggctat ggggtgaggc tccctcgcct tgctcgtaaa ccaggatgtc
     7381 attttttgct aatattgggg atatggcatt tgacgtgcgg cgaagctggt cgtaagaagt
     7441 gaaaagtacc agtgtccgcc cgccgatggc acggcacagt ccaatgagtc cgctttccac
     7501 gccacgctgg tgaccttggc ggtcacctgg ctcggggata tcattagcaa cgaatagaag
     7561 ggcggcattt tcataatcaa atggcgaacc tacggagagc tcatcggcct cctcggcgta
     7621 caagcggtgt cgtaagtagg tgaactcccc agcggcagtc agtgtggcag atgtgagaat
     7681 cacagcggat ttttcgtgcc atagatagcg ctgcataagc ggaccgatct ccaggggtgc
     7741 ggcgttcaaa gcgacgctgg agctatttgt gttggcttct gcccaataga tgagcccttg
     7801 atccggcttg aagaccatcc cttccatgtt gtcgtgcaat tcagtgaaac ggtgatacaa
     7861 gttgctaagc gcgctgaaaa gttcctcgtc cccttctggc agggctggca aaatctctga
     7921 tagcccttgg agtagcttgg cgagcacgtc aagcatggga gagagcgtgt gctcggcttc
     7981 gtcccaggca gcttcaacgc ttgcccaggt agaaagggtg cgtgtggcgg gttggatgcg
     8041 cacttggtgg gaataggcgc ctacttgacg gccttcacgt tgctcggtga ggaattcgtc
     8101 taaggaacta aaaaagaggc gcga
//
LOCUS       DBNHOH010000731         1489 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_814003, whole genome shotgun sequence.
ACCESSION   DBNHOH010000731 DBNHOH010000000
VERSION     DBNHOH010000731.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_814003"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="AC16V7_12730"
     CDS             <1..798
                     /locus_tag="AC16V7_12730"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_process: GO:0006555 - L-methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HIR6258032.1"
                     /translation="GASLLAEAGADVINIADSPMARMRMSPWAACSQIQRQFDIETVL
                     HFPTRGRNLLRVQGDLLAAHALGIRNVFVVMGDPTAIGDYPEAMDNYDLVPSGLIKLI
                     KKNFNVGLDHAGADLGQPTSYFVGCALNLTPQVAEQEMKNLRRKMNAGADFILTQPVY
                     QPEVAGTFLTAYQDRYGPLVPILVGVLPLYSARHASFLHNEVPGVSIPENIQQRMRQA
                     GEDAPSEGVRIALELIEQVQPWVSGVYLMPQFSRFDLAAVIVEGCRR"
     gene            complement(910..1206)
                     /locus_tag="AC16V7_12735"
     CDS             complement(910..1206)
                     /locus_tag="AC16V7_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258033.1"
                     /translation="MDKKTTGIIATVATGLLCGCPGLISLCMGAMFAVASQIPSAEID
                     MFGSSNPSDALTFGVGGICLGVVFVAIPIVVGVVMLRQKQEAVPVSDEPLPPPI"
     gene            complement(1288..>1489)
                     /locus_tag="AC16V7_12740"
     CDS             complement(1288..>1489)
                     /locus_tag="AC16V7_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014482820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta-related
                     protein"
                     /protein_id="HIR6258034.1"
                     /translation="AGGDTLSSVSLFDVYRGQQVGDGKKSLAYSLTYQAPDRTLTDSE
                     VAQIRRRIVRRLEEVLGAKLRS"
BASE COUNT          330 a          448 c          402 g          309 t
ORIGIN      
        1 ggagccagcc tgctggctga ggcaggcgcc gatgtgatca acatcgccga cagccccatg
       61 gcacgcatgc gcatgagccc ctgggcggct tgcagccaga ttcagcgcca gttcgatatt
      121 gagaccgtgt tgcacttccc aacccgaggt cgcaacctgc tgcgcgtaca gggcgacctg
      181 ctggcagctc acgcgctggg catccgcaat gtcttcgtgg tgatgggcga tcccaccgcc
      241 atcggcgatt accccgaggc gatggataac tacgacctgg tgccctccgg gctgatcaag
      301 ctgatcaaga agaatttcaa cgttgggctg gatcacgctg gagcagacct ggggcaaccc
      361 acctcctatt ttgtaggctg cgccttaaac ctcaccccgc aggttgccga gcaagagatg
      421 aaaaatcttc gccgcaagat gaacgccggg gcagatttca tcctcaccca gccggtatat
      481 caaccagaag tagcggggac gtttttaaca gcctaccagg atcgttatgg cccgctcgtt
      541 ccaatcctgg tgggtgtgtt acctttatac agcgcccgcc acgcttcatt cttgcacaac
      601 gaagttccgg gcgtctcgat ccccgaaaac atacagcagc gcatgcgcca agcaggggaa
      661 gatgcaccca gcgaaggcgt tcgtatcgcc ctggagctga ttgagcaggt gcaaccttgg
      721 gtcagcggcg tgtacctgat gccgcaattc agccgcttcg atctggcagc agtgatcgtc
      781 gaaggctgcc gcagataatt aattcggaaa acttagaaag acggggtact gatgtgtgac
      841 acacctcccg aaacattaaa ttcgggaggt gtcataatca tctccccaac cgcgaaacac
      901 cagtaaatac taaattggag gaggcaacgg ctcgtccgag accgggacgg cttcctgctt
      961 ttggcgcagc atcaccacgc ccaccacaat cgggatggcg acgaacacaa cccccaggca
     1021 gatgccgccg accccgaagg tcagtgcgtc cgacggatta ctgctcccaa acatatcaat
     1081 ctcggcactg ggtatctggc tggccacggc gaacatagcc cccatgcaca ggctgatcaa
     1141 tccagggcaa ccacacagta atccagtcgc tacagtggca atgataccgg ttgttttttt
     1201 gtccatggca ttctccttga tcttggggga ggcaggatgc agctttctaa ttgcgcatcc
     1261 caactccccg ttttgctttg atgatgatta gcttcgcagt ttagctccca gcacttcctc
     1321 aagcctgcgc acgatacgcc ggcggatttg ggcgacttca ctatcggtca gtgtgcgatc
     1381 gggggcctgg taggtgaggc tataagccag gctctttttc ccgtcaccaa cctgctgacc
     1441 gcggtagaca tcgaacaggc tcacgctgct cagtgtgtct cctccagcc
//
LOCUS       DBNHOH010000732         8022 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_108892, whole genome shotgun sequence.
ACCESSION   DBNHOH010000732 DBNHOH010000000
VERSION     DBNHOH010000732.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8022)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8022)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8022
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_108892"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..602)
                     /locus_tag="AC16V7_12745"
     CDS             complement(48..602)
                     /locus_tag="AC16V7_12745"
                     /inference="COORDINATES: protein motif:HMM:NF042609.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0758 domain-containing protein"
                     /protein_id="HIR6258035.1"
                     /translation="MTSQRPSYRITDLDEAERPRERLQRLGAQALSSAELLAILLRVG
                     VHGENAVQVGQRLMQTFGGLPGLHRASFDEVCAQRGIGEAKAAQVKAAIELGRRLAAE
                     APDERPAIHSPQDAADLVRYEMGALEQEELRVILLDNRNRMITVEAVYRGSVNSSQVR
                     VGELFKSTSQFDTTGCVFQQLTRI"
     gene            799..1311
                     /locus_tag="AC16V7_12750"
     CDS             799..1311
                     /locus_tag="AC16V7_12750"
                     /inference="COORDINATES: protein motif:HMM:NF024183.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR6258036.1"
                     /translation="MTFDPSAFSNVILIVTAWGGAFIAALWLSLVIWTYRDIRGRARD
                     PLGRILAVLVVAVLFLPGIVIYLILRPARTLEEEYQQSLEEEALLHSIEDASLCPGCG
                     RRIKENWMVCPGCHTKLKKNCHQCGKLMELGWNLCPYCGTPAPGMRREGLTMDDALRP
                     MPIEEEQASE"
     gene            complement(1394..2683)
                     /gene="eno"
                     /locus_tag="AC16V7_12755"
     CDS             complement(1394..2683)
                     /gene="eno"
                     /locus_tag="AC16V7_12755"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR6258037.1"
                     /translation="MDLIIEDVVGREILDSRGNPTVEVEVTLMDGSWGRAAVPSGAST
                     GVHEALELRDGDKARYGGKGVLKAVEHVNNQIADLLLGWDASDQKGIDAALLELDGTP
                     NKANLGANAILGTSLALAKAAANALGLPLYRYIGGVYAHVLPVPMLNILNGGAHTSWQ
                     STDAQEFMVMPLGASSFAEGLRWGAEIYHALKTVLKGRGYTALVGDEGGYAPALKANA
                     EAVEVILEAIQKAGYKAGEQVAIALDPAASELYEEDTGLYNLRKEGKKLTGEQMVAFW
                     KSWVDQYPIVSIEDGLAQDDWESWQLMVQELGDRLQIVGDDLLVTNPDRVRRAIKEKA
                     ANALLVKLNQIGTLTETIEAVETCQRAGWRAVTSHRSGETEDSTIADLAVALNMGQIK
                     TGAPARSDRVAKYNQLLRIEAELGETAKYAGWSALNK"
     gene            2800..3486
                     /locus_tag="AC16V7_12760"
     CDS             2800..3486
                     /locus_tag="AC16V7_12760"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR6258038.1"
                     /translation="MSAETSPELTDPRDRLTYHFDYTPFRRVLTSTVQTLFHLIAIID
                     ASGVEHLPKEGPVVLAANHVTNFDVFPMQFSLPRPIFFMAKAELHRNPILDVALRQMG
                     SFPVERGARDEWALRHAQKVLEHRQMLGIFPEGKRNMGRGLRPAKTGAARLALSVNCP
                     IVPLAVEGTQRMFQRFPRRTLVHVTVGEPIFPQHGDTALSLTDQMMFSLAELLPPEQR
                     GVYAQHPTGF"
     gene            complement(3525..5174)
                     /locus_tag="AC16V7_12765"
     CDS             complement(3525..5174)
                     /locus_tag="AC16V7_12765"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR6258039.1"
                     /translation="MATKFIFFTGGVVSSVGKGVTAAAIGRLLRERGFNVAVQKLDPY
                     INVDPGTMSPYQHGEVFVLDDGAETDLDLGHYERFIDISLNSVCNVTTGQVYADVIGK
                     ERRGDFLGGTIQVIPHITNEIKRRISLVAKTTGAEIVLVEIGGTVGDIESLPFLEALR
                     QMRSDLGRENTVYIHVTWLPYIGATRELKTKPTQHSVRELRSIGISPDVIVARSDYPV
                     DEDLCEKIALFCDVERKAVFPMVTTDVLYEIPLLLERQHAGDYLLDRLGLKPRRQPDW
                     QEWEQLVQEVRKSKPVVNIALVGKYVELQDAYMSVREALKHAAISQGVEVEISWVHSA
                     ELEKGRSWEMVRNADGIIVPGGFGSRGIEGKIQAAHYARENKIPYLGLCLGMQLMVIE
                     FARHVLGKEEVNSTEFDRSTPYPAIDLMPDQKSISDMGGTMRLGLYPCQLQPGSIAAK
                     AYQKKVVQERHRHRFEFNNAYREILAKAGMRYSGISPDGRLVEIAELTNHPFMLGTQF
                     HPEFLSRPNRPHPLFVAFVKAVRERSGVQVGLEVEKLKIEG"
     gene            complement(5272..5622)
                     /locus_tag="AC16V7_12770"
     CDS             complement(5272..5622)
                     /locus_tag="AC16V7_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HIR6258040.1"
                     /translation="MVDRLREMQASSPEIEASAVVSVDGLIIASALPADAEEDRVSAM
                     SAAMLSLGERIANELGRGTLDQVYIRGNSGYVILSAIGEEAVLTALAREGAKLGLVFL
                     EMRRAAEDLLRLVE"
     gene            5887..6522
                     /locus_tag="AC16V7_12775"
     CDS             5887..6522
                     /locus_tag="AC16V7_12775"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009785910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR6258041.1"
                     /translation="MRIYDISLPILPGMPVWPGDPQVIMDRVGNMDEGADANVTHLSM
                     SVHTGTHVDAPYHFMGGEAATVDLLSLKLLTGRAYVLHLPDIDLITADVLEQADIPPR
                     TRRLLFRTRNSDHWGNPSHEFHTNFVAISSDGAQHLVDRGVKLVGVDYLSVAPYGDST
                     PTHRILLSAGMLIVEGLDLSGVSQGRYTLHCLPLKLVGCDGAPARAILIGV"
     gene            6537..6974
                     /locus_tag="AC16V7_12780"
     CDS             6537..6974
                     /locus_tag="AC16V7_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HIR6258042.1"
                     /translation="MKLSSPAFAEGDAIPRKYTCDGDNVSPALSWQGAPQATKSLVLI
                     MDDPDAPSGTFVHWILFDIPPAFSLLREGVRGVGMPGANSYRKVGYNGPCPPPGPAHR
                     YFFKVYALDQLLRLRPGVIKAELEGAMQGHILDSGQLMGRYGR"
     gene            complement(7015..7459)
                     /gene="rnpB"
                     /locus_tag="AC16V7_12785"
     ncRNA           complement(7015..7459)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="AC16V7_12785"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            7816..>8022
                     /locus_tag="AC16V7_12790"
     rRNA            7816..>8022
                     /locus_tag="AC16V7_12790"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1762 a         2204 c         2142 g         1914 t
ORIGIN      
        1 cgagttctcg tttttagctc gcgttcgcat ccgcgaattg ggttgtgtta tatacgggta
       61 agctgttgga aaacacaacc agtcgtgtca aattgagaag ttgatttgaa cagctcgccc
      121 acccgcacct gggaggaatt taccgagccg cgatagaccg cttcgacggt gatcatccgg
      181 ttgcgattgt ccagcagaat cacgcgcaac tcctcctgct ccaatgcgcc catctcgtag
      241 cgaaccaggt cggcggcatc ctgcggactg tgtatcgctg ggcgctcatc cggggcttca
      301 gcagccaggc ggcggcccag ctcgatggct gcttttacct gggcagcctt ggcctcgcca
      361 attccgcgct gggcgcacac ctcgtcaaac gaagcgcgat gcagccccgg caaaccgcca
      421 aaggtctgca tcaagcgctg cccgacctgc acagcgttct cgccgtgcac tcccaccctg
      481 agcaggatag ccaatagctc agcggatgag agcgcctggg cacccagcct ttgtagtcgc
      541 tcgcgcggcc gttcagcctc gtccaggtcg gtgatgcggt aggacgggcg ttggctagtc
      601 atggtgaatc aaggtgggtt gaatgttgcg gaagggggtg gggttgtcca ttggcaatct
      661 cctgcaagtt tggcagatga agttatcttg gacagtcgag ctacctggta gaagtattcc
      721 ccggcagcac tatataattt tactctggaa gtcatctgat ctccaataat gctataatgt
      781 actcccaggg agcctgctat gacctttgat ccgtctgcct tcagcaatgt tattctcatc
      841 gtgaccgcct ggggaggggc gttcatcgct gccctttggc tgagcctggt aatctggacc
      901 taccgcgata tccgcgggcg ggcgcgcgac cctcttgggc gcatcctggc ggtgctggtg
      961 gtggcggtgt tgtttttacc cgggattgtc atctacctga tcttacgccc ggcgcgcacc
     1021 ctggaagagg aataccagca gtcccttgaa gaggaagcct tgctgcactc gatcgaagat
     1081 gccagcctgt gtccgggttg cggcaggcgc atcaaggaaa actggatggt ttgcccaggt
     1141 tgccatacga agctgaagaa aaattgccac caatgcggca agctgatgga gctaggatgg
     1201 aacctgtgcc cttactgtgg tacgcctgcc cccggaatgc gccgcgaagg tctgaccatg
     1261 gatgatgccc tgagacctat gccgattgaa gaagagcagg cttccgagta aatgtagtta
     1321 ttaacacgaa ggacaccaag gaatcataaa gggtgtttta gattccttgg tgcccttttt
     1381 gttttatttg ttcttattta tttaacgcac tccaccctgc atattttgcc gtctcaccga
     1441 gctcggcttc gatgcgcagc aactggttgt atttggcaac acgatcggag cgagctggag
     1501 caccggtctt gatttgcccc atattgagcg ccaccgctag gtcggcgatg gtggaatcct
     1561 cggtttcgcc tgatcggtgt gaggtgactg ctcgccagcc ggcgcgctgg caggtctcca
     1621 ctgcttcaat ggtctcagtg agcgtgccga tctggtttag cttaaccaac aaggcattgg
     1681 cagctttctc cttgatggca cgacgcaccc gatcggggtt cgtgaccagc aggtcatccc
     1741 ccacgatttg aagacggtcc cccagctcct ggaccattaa ctgccagctt tcccagtcat
     1801 cctgagccag cccatcttca atcgagacga ttggatactg gtcgacccag gatttccaga
     1861 aggcgaccat ctgctcgccg gtcagctttt taccctcttt tcgcaggttg tacaagccgg
     1921 tatcttcctc gtaaagctcc gatgccgctg gatcgagagc aattgctacc tgttccccag
     1981 ccttgtagcc agctttctga atagcctcga gtataacctc gaccgcctcg gcgttagcct
     2041 tcaatgcagg cgcataaccg ccttcatcgc cgaccagggc ggtgtaaccg cgtcctttta
     2101 gaacggtttt gagggcgtga tagatctctg ctccccagcg tagaccttca gcgaagctcg
     2161 atgcccccag aggcattacc atgaactcct gggcgtcggt ggattgccag cttgtatggg
     2221 cgcctccatt aaggatgttt agcataggta caggaagcac atgggcatat acgccaccaa
     2281 tgtagcggta gagcggcaag ccaagagcgt tagccgcagc cttggcaagc gcaagactgg
     2341 tgccaagaat ggcatttgcg cccaaattgg ccttattggg tgtcccatcc agctcgagta
     2401 aggcagcgtc aatgcctttc tggtcactgg cgtcccagcc cagtagcagg tctgcaattt
     2461 gattgttgac gtgctcaaca gctttcaata cgcctttccc cccatagcga gctttgtcgc
     2521 catcgcgcaa ttccagcgct tcgtggacgc cggtggatgc ccccgaaggc actgctgccc
     2581 gcccccagct tccatccatc agggtcacct ccacctcaac agtggggttg ccgcgcgaat
     2641 cgagaatctc acgaccaacg acatcttcga tgatcaaatc catagttcac ctcgttgata
     2701 ttgaaaaagt gatgcagttg aatgtttatc cttcaaccgc gttaatccga tatccgttaa
     2761 aatattgatt aagccgaaat gcaagtataa tgcaattcaa tgtctgcaga gacctcccca
     2821 gaacttacag atccccgcga tcgccttacg taccattttg attatacgcc ctttcgccgc
     2881 gtattgacat ctaccgtcca gaccctattc catctcatag cgattatcga tgcgagtggt
     2941 gtggaacacc tgcccaaaga agggccggtg gtgttagctg ccaaccatgt caccaacttc
     3001 gatgtcttcc caatgcaatt tagcttgcca cgaccgatct tttttatggc taaggccgaa
     3061 ttgcatcgca atcctatctt ggacgttgcc ctgcgccaga tgggtagctt cccagtggag
     3121 cgcggcgccc gggatgaatg ggcgctacgc catgcccaaa aagtgctgga acataggcag
     3181 atgttgggca tcttcccaga aggaaagcgc aatatgggtc ggggcttgag accagccaag
     3241 actggtgctg ctcgattggc tttatccgtg aactgcccga tcgtgccgct ggcagttgaa
     3301 ggaacacagc gcatgtttca acgctttccg cgccgtacgc ttgtgcacgt cacggtaggt
     3361 gagcccattt tcccacagca tggcgataca gccctgtcgc tcaccgatca gatgatgttc
     3421 tcactggctg agttgttgcc accagagcag cgtggcgtgt acgcgcaaca tcccactggg
     3481 ttttaatacc aacaaacccg agctattccc caggtttaat ttttctagcc ttcaattttt
     3541 aatttttcga cttccaagcc tacctgaacc ccagaccgct ctcgcacggc tttcacaaag
     3601 gcgacgaaca ggggatgtgg tcgattggga cgtgagagga attctggatg aaactgcgtc
     3661 ccaagcataa agggatggtt ggtcaattcg gcgatctcca ccagacgacc gtcgggggag
     3721 atgccggaat agcgcattcc cgctttagcc aatatctccc gataagcatt gttaaactcg
     3781 aagcgatggc gatgacgttc ctggacgacc ttcttctgat aggctttggc ggcgatgctt
     3841 ccgggctgca attggcacgg gtacaatccc aagcgcatgg tgcctcccat atcggagata
     3901 cttttttgat cgggcattaa atcgattgcc gggtagggtg tggaacgatc gaactcggta
     3961 gaattgacct cttctttgcc caaaacgtgg cgagcaaact caataaccat cagctgcatg
     4021 cccaggcaaa gtcccaggta gggaattttg ttctcgcggg cgtagtgtgc tgcctgaatt
     4081 ttgccctcaa tcccgcggct cccaaacccg cccgggacga taatgccatc cgcgttacgg
     4141 accatttccc aactgcggcc tttctccagt tcggcagagt gcacccaaga tatctcaact
     4201 tcgaccccct gtgagatggc agcatgtttt aacgcttctc gcacactcat gtaggcatct
     4261 tgtaattcga catatttccc gaccaaggcg atattcacaa caggtttgga cttgcgcact
     4321 tcttgtacca actgctccca ttcctgccag tctggctggc gccgcggctt aagacctagc
     4381 ctgtctaaaa ggtaatcacc agcatgctgg cgttcgagca gcagagggat ttcatataat
     4441 acatctgttg tcaccattgg gaagactgcc ttgcgttcga catcgcagaa gagggcgatt
     4501 ttctcgcaca ggtcctcatc gacgggataa tccgaccggg cgactatgac gtcgggtgag
     4561 ataccaatgg agcgcagttc acgcaccgaa tgctgcgttg gcttggtttt aagttcacgg
     4621 gtcgccccga tgtaaggcag ccaggtcacg tggatgtaga cggtattctc ccgccctaaa
     4681 tcgctacgca tctggcgcaa ggcttccaga aagggtaagg actcgatatc acccaccgtc
     4741 ccgccaatct cgaccagcac aatctcggcg cccgtagttt tggcaaccaa gctgatgcgg
     4801 cgtttgatct cgttggtgat atgcgggatg acctggatcg tgccacccaa aaaatcccct
     4861 cgtcgctctt taccaatcac atcggcatag acctgccctg ttgtaacgtt acatacactg
     4921 ttcaggctga tatcaatgaa tcgctcataa tggcccaggt ccaggtcagt ctcggcaccg
     4981 tcatcaagga cgaacacctc accatgctgg taagggctca tcgtgcccgg gtccacgttg
     5041 atgtatgggt cgagcttttg cacagcgacg ttaaagccgc gctctctaag gagtcgccca
     5101 attgctgcag cggtgacacc tttgcctact gagctaacta ctccaccggt aaagaatatg
     5161 aacttggttg ccattcgtgc ctccattggt cggtgttaaa ggaagtgggg aggtccgttt
     5221 agcggggacc tccccacgag ggtgtcaata taagggaacc ttgctggtaa atcattctac
     5281 caacctcaat agatcttctg ctgctcgccg catttcgaga aaaaccaatc ctagcttggc
     5341 accttcgcgc gccaatgcgg ttagcacagc ctcctcaccg atggcggaca gaattacgta
     5401 accgctgttg ccccggatat atacctggtc caaggtaccc ctgcccagct cgttggcaat
     5461 gcgctcaccg aggctgagca tggcagcaga catcgctgag actcgatctt cttctgcatc
     5521 ggctggtagc gcagaggcta tgatcagccc atctacagaa actacagcgg aggcttcgat
     5581 ctcaggagat gacgcctgca tttcgcgaag gcggtcgacc atcaattctg ttcgcgagcg
     5641 ggtcaaggcg atgtccactc ctcatgagga ttgattgaac ccgccatgag tctaccataa
     5701 ctgatagact aatgcaaaag caggttcttc gttgtctgag atattctggc tggcgtcgct
     5761 gcaaaggttg gaggccaatt catccttaat ttggcggcgc atgacgagaa ttctcacaca
     5821 gaccacgcca ttaagttgct ttttaaaatg ccttgggcta tactttttaa taatcacgga
     5881 gggagtatgc gcatatatga tatatccttg cccatattgc ctggaatgcc cgtgtggccc
     5941 ggagacccgc aggttattat ggatcgggta gggaacatgg acgaaggcgc ggatgccaat
     6001 gtgacacatc taagcatgag tgtccataca ggcacgcacg ttgacgctcc ctaccatttt
     6061 atgggggggg aggcggcaac cgttgaccta ttatcgctca agctgttgac tggcagagcc
     6121 tatgtgctgc atttgccaga catagatctc atcaccgcag atgtgctgga gcaggcggat
     6181 atccctccac gcacgcgtcg cttgctcttt agaacgcgta actctgatca ttgggggaat
     6241 ccttcgcatg agtttcatac caattttgtg gccatcagct cggatggcgc ccagcatctg
     6301 gtggatcgag gcgttaagct agtgggtgtg gactatttgt cagtggctcc ttatggcgat
     6361 agtacaccaa cacaccggat tttgctgagc gcaggcatgc tgattgtgga ggggctggac
     6421 ttgtctggcg tctcgcaagg ccgctatacg ctacattgtc tacccctcaa attggtcggc
     6481 tgcgacgggg cgccggcacg ggcgattcta attggcgttt agcaggaggt aagccaatga
     6541 aattaagcag tccggctttc gcagaaggag atgccatccc acgcaagtat acctgcgacg
     6601 gcgataatgt ttctccagca ctctcctggc agggagcccc gcaggctaca aagagcctgg
     6661 tgttgatcat ggatgaccca gatgccccat ccgggacctt tgttcactgg attctattcg
     6721 acatccctcc cgcattcagt ctgctgcgcg aaggcgtgcg gggtgtgggc atgccagggg
     6781 caaacagcta tcgcaaagtg ggctacaatg gtccctgccc gccgccaggc ccagcgcacc
     6841 gctatttctt taaagtatat gcactggatc aattgctcag gttgcgtccg ggggtcatca
     6901 aagccgagct ggaaggcgcc atgcaggggc atatcctaga ctcaggccag ttaatgggtc
     6961 gctacgggcg gtaattctag cgtttgcaga gcgttagttt tcaggaataa gaaaatccca
     7021 ggccagtcta taagccgcat tctgtagggc aggaacctgc cctggtgatc atctctctgg
     7081 gctgcgcgtc tccgcgcagc tcttgcagcc tacccgggac tttggaccgg agtcctggag
     7141 acgagcaatc tccttagatt gctgattata gatatccaat tgctgattgg ttcttcaacc
     7201 aacaatctgc aatcatcatt caacaatcct cttgtccctg cttggccttg ctcctggcgg
     7261 gggttgcctg gccgaccgca ttgctgcggg cgccggtggt ctcttacacc accttttcac
     7321 ccttgccgaa ccggcggttt gtttctgtgg cccgatccaa caggttaccc cgttccgggg
     7381 attacccggc gccgtgctct atggagtgca gactttcctc gactccggaa aacggagccg
     7441 cgatcacccg accaacctgg gtgagaatca tgataacata tcttgacaga attgtcaggt
     7501 gaagtataat agggattgca aacgacaatc gaggtctcgc ccgaacaggg aagatcgatt
     7561 acaactgagc aggacgcctc tgcgccagag accgaagcga aaccgcttta ggtctgacgg
     7621 gccgatgtca gagagtatgg gcatcggcag ttgtgtctgt gtgcaaatcg acttagagcc
     7681 ttgacaggcg taagtgaggc tggtacaatc cagtccgctt gaaagaccag gaccttaaca
     7741 actgaagagt gatagaaagt cagccttgtc ttttccagga cttccgtaac cataacgcac
     7801 taagtgcgta aaagttttgc ggagagtttg atcctggctc aggatgaacg ctggcggcgt
     7861 gcttaataca tgcaagtcga acggggactt tgtattcgta catagtccta gtggcgaacg
     7921 ggtgagtaac gcgttggtga cctgccccaa agtgtgggat aacagtccga aaggattgct
     7981 aataccgcat gtggtcatcg ggtttagaag ccgatgacta aa
//
LOCUS       DBNHOH010000733         3354 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1647605, whole genome shotgun sequence.
ACCESSION   DBNHOH010000733 DBNHOH010000000
VERSION     DBNHOH010000733.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1647605"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..517)
                     /gene="rpsP"
                     /locus_tag="AC16V7_12795"
     CDS             complement(176..517)
                     /gene="rpsP"
                     /locus_tag="AC16V7_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIR6258043.1"
                     /translation="MVKIRLRRMGAKGQPTYRIVAADKESPRDGRFLEILGSYNPRTV
                     PATILVKEDRVYHWMSQGAQPSDSVARLFRTTGLLDRYARYKQGEELETLLAEAAAEA
                     AANPISPKTRQ"
     gene            complement(625..1947)
                     /gene="ffh"
                     /locus_tag="AC16V7_12800"
     CDS             complement(625..1947)
                     /gene="ffh"
                     /locus_tag="AC16V7_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR6258044.1"
                     /translation="MFENLTERLENVFKQLRRRGKLSESDVDAAMREVRLALLEADVN
                     YAVVKDFVARVRQRAIGYEVSKALNPGQQVVKIVNEELIATLGAPDRFDLSGPKPRTI
                     MLVGLQGSGKTTAAGKLARLLRAQGERVMLVACDPYRPAAVRQLETLGERLNVPVHFE
                     EGIQPPQLAVRARDKAQKGGFTVLILDTAGRSQLDDALMNELQEIRRRVDPVETLLVV
                     DAMIGQEAVNIAKGFRDVVTLTGLLLTKMDGDARGGAAISIRSVTGVPIKFLGTGEAL
                     DAIEAYDPARLSSRILGMGDMIGLIEKAEAAFDEKVAAEQANRMLSGEFTLQDFADQL
                     SQLRKMGPLGQLMDMLPGGMGQMARQINPQDAERQFKRTEAIIGSMTKDERRKPDILN
                     ASRRRRIASGSGTHVQDVNRLIKQYRDAQRLFKSLKKSGKRGIPRLFG"
     gene            2105..2494
                     /locus_tag="AC16V7_12805"
     CDS             2105..2494
                     /locus_tag="AC16V7_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR6258045.1"
                     /translation="MLQDKSSPVIDDPAKLFKILMHPARLSILDILRDSEHCVCHMES
                     HLGYRQAYISQQLMVLREAGLVEDRKDGLNVFYRVTVPTLYEVIDVARKITYVDEQVQ
                     EHDPHHEAKAASCPCPKCNPAAKNVSC"
     gene            2610..2873
                     /locus_tag="AC16V7_12810"
     CDS             2610..2873
                     /locus_tag="AC16V7_12810"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024590.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR6258046.1"
                     /translation="MLEIKILGTGCAGCLKTEQVVHEALRALGIQNANVELVAEQRMI
                     EYGLLADRAPGLLINGYLAWAGTVPTIEQVMEWVTQAVTPTVI"
     gene            2935..>3354
                     /locus_tag="AC16V7_12815"
     CDS             2935..>3354
                     /locus_tag="AC16V7_12815"
                     /EC_number="1.4.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF013743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HIR6258047.1"
                     /translation="MTDFLQITVDKFTFKVATDRYYNEEGVWALVQGNRVRIGLSDFL
                     QQRSGDVAFAEITPQGTVLAVGDEIAVIETVKVNISLSSPVSGSIVEINPLMETAPEI
                     INQDPYGEGWVAIIEATQWESDSSRLLDPQAYYSRMQI"
BASE COUNT          828 a          884 c          812 g          830 t
ORIGIN      
        1 ctggaattgg gaaacctgca cctgggtagg atcatccacc agtgatgttg caatatatga
       61 gattaattcc ttcaccatcc gcctcctatc aagttatcag cagagaaact acaccctgct
      121 tggtcagctt tcctgttata tacaaaaagc gtctttggat caggcttact tagctttact
      181 ggcgcgtctt ggggctgatg gggtttgccg cagcttctgc ggcagcttcg gccaacaggg
      241 tctccaattc ttcaccctgc ttataacgtg cgtaacggtc aagcaaaccc gtggtgcgga
      301 acagtctagc cactgaatcg ctaggctgag ctccctggct catccagtgg tatacgcgat
      361 cctccttcac cagaatagtg gctggaactg tgcgcggatt ataggaacct aggatttcca
      421 ggaagcgccc atcgcgcgga ctttctttgt ctgcagccac aatgcgatac gttggctggc
      481 ctttggctcc catgcggcgc aatcgaattt ttaccattga actttctcct tcaataaata
      541 tgttctaaat atatctcaaa atggttgggg tattttacag cggaaagtcc aatgcaaaat
      601 acctgctcat tttgttctta ccttctaacc aaacaaacgt ggtatgccgc gtttgcccga
      661 tttttttaat gatttaaaga ggcgctgtgc atcgcggtat tgcttgatca gacgatttac
      721 atcctgaaca tgcgtaccgg atccactggc gattcgacgc cgacggctag cgttgaggat
      781 atccggcttc cgccgttcat cctttgtcat cgaaccaatg atggcttccg tccttttaaa
      841 ttgtctctcg gcatcttgtg gattgatctg ccgagccatt tggcccatcc cgccgggaag
      901 catgtccatc aactgtccca atgggcccat tttgcgcaat tggctgagtt ggtcggcaaa
      961 gtcctgcaga gtgaactcac cagagagcat cctgtttgcc tgctccgcgg ccaccttctc
     1021 atcaaaggct gcctcagcct tttcgatcaa tccgatcatg tcgcccatgc cgagaatacg
     1081 ggaagacagg cgagccggat cgtatgcctc aatggcatcc aaggcttcac ctgttcccag
     1141 gaacttgatc ggcacaccag tgaccgagcg aatagatatt gccgcaccgc cacgagcatc
     1201 gccgtccatc ttggtcaaga gcaggcctgt caatgttacc acatcgcgaa atcctttggc
     1261 gatattgact gcctcctggc caatcatggc atccaccacc agcagggtct cgaccgggtc
     1321 gacgcgacgc ctaatctctt gaagctcgtt catcaacgca tcatccaact gagagcgccc
     1381 ggctgtatct aagatcagga cggtaaagcc tcccttttgg gctttgtcgc gtgcgcggac
     1441 cgccagttga ggaggctgga tgccctcttc gaagtgcaca gggacgttca ggcgctcgcc
     1501 taaagtctct agctggcgca ccgccgccgg ccgataggga tcgcaggcta ccaacatcac
     1561 cctctcaccc tgggcgcgta gtaggcgggc tagcttacca gctgccgtgg tctttccaga
     1621 gccctgcaga ccgaccagca taattgtgcg cggcttgggg ccactcaggt caaagcggtc
     1681 tggagcaccc agcgtggcaa tcaactcttc gttaacgatc tttaccacct gctgccctgg
     1741 gttgagcgct ttagatacct cataaccgat ggcgcgctgg cgtacccgag ctacaaaatc
     1801 ctttacgacg gcataattga catcagcttc aagcagcgcc aagcgcactt cgcgcatggc
     1861 ggcatccaca tcgctctccg agagcttccc tctccggcga agttgcttga agacattttc
     1921 gagacgttcg gtcaggttct caaacacagc attgcatttt agtatggaag caaggaatgg
     1981 tcaagtagac atttagaaaa accgcggggc aacgagaatg gaagttggat ctcttaaaca
     2041 agtgaacgcc cgatgaaaat aattcaaaat attggttata attacattca aaggattgaa
     2101 tattgtgctt caagataaat cttcacccgt gatcgatgat ccagccaagc tattcaaaat
     2161 tctcatgcac ccggctcggt tgtctatatt ggatatttta cgcgatagtg aacactgtgt
     2221 ctgccacatg gaatcacacc tggggtaccg gcaggcatat atctcgcagc aattgatggt
     2281 cttacgcgaa gccggcctgg tggaagatcg caaagatggg ctgaacgttt tttatcgtgt
     2341 gaccgtccca accctttacg aggtaatcga tgtggctcgt aaaatcactt acgttgacga
     2401 gcaagtacag gaacacgatc cccaccacga agcaaaagct gcttcctgcc cttgccccaa
     2461 gtgcaatcca gccgctaaga atgtatcttg ctaagtccgt acagcagatg tcatagcaaa
     2521 cctgtgattt tattcactat gtctaattaa ttgaaagaga tatactatat tcaatgattt
     2581 gaatataact tcatgcatag gacaatgcca tgctggagat caagatttta ggcactggct
     2641 gcgccggatg cttaaaaaca gaacaagtag ttcacgaggc cttacgagcg ctgggcattc
     2701 aaaacgctaa cgtagagctt gtcgccgagc agcgcatgat tgagtacggt ttgttagcag
     2761 atcgtgcgcc aggtcttttg atcaatggat atttggcttg ggcgggtaca gtgccaacaa
     2821 ttgaacaggt catggagtgg gtcacgcaag cagtaacccc aactgtgatc tagactacac
     2881 gattattttg cgtatccttt acccaagccc aacctggaca tccaagagta tgacatgaca
     2941 gatttcctcc aaatcaccgt cgataaattc acttttaaag tcgctaccga ccgttactat
     3001 aatgaagagg gggtttgggc gctagtccaa gggaaccgcg tgcgcattgg attgtccgat
     3061 ttcctgcaac agcgtagcgg agatgtggcc ttcgctgaaa ttacgccgca aggaactgtg
     3121 ctggcagtgg gcgatgaaat agccgtgatt gagacagtca aagtaaatat cagcctctca
     3181 tcaccggtat caggatcaat cgtcgagatc aatccgctca tggaaacagc cccggaaatt
     3241 atcaaccaag atccatatgg tgaaggttgg gtagcaatta ttgaggccac ccagtgggag
     3301 agcgactctt cccgcctgct cgatccacaa gcgtactact cccggatgca aatc
//
LOCUS       DBNHOH010000734         2300 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_237640, whole genome shotgun sequence.
ACCESSION   DBNHOH010000734 DBNHOH010000000
VERSION     DBNHOH010000734.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_237640"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="AC16V7_12820"
     CDS             complement(<1..501)
                     /locus_tag="AC16V7_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258048.1"
                     /translation="MNDHRTKHLNILVLLLSAVLIAGCSAPERTPEFTPVATVTQVVL
                     PTPNIITITATPTSTVTPEPTITATSTSTSTPDPYLEYSIDYLANRGYGGGELRVEEA
                     MAANSYFTRTLVSYPSDALRIYGFMNTPRRVSVEAGEKLPLVIALHGYIDPAVYATLD
                     YTTRYAD"
     gene            complement(591..1841)
                     /locus_tag="AC16V7_12825"
     CDS             complement(591..1841)
                     /locus_tag="AC16V7_12825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6258049.1"
                     /translation="MIDFETPKPIAQQSVALKTVAENMMRPVSRYFDEHEHEIPWDYI
                     NFMHMAMRATGAGSLTPRERKKNGDEPKKDRPPIGYQSLAHLLEILAWGDVGIYLVTP
                     GGGLGAAAVEATGTPEQKEKFLARFKEERPTFAAMAMTEPQAGSDTSAIRSTAVLDKS
                     TNEWVLNGEKIFVTAGHKSLCESNGFVVIWATIDPKAGRAGMRPFVVEASAPGVQVTK
                     LEHKMGIRASDTASIVMQDCRVPFENILGSPEVEVETTKGFKGAMATFDATRPLVAAT
                     GVGVGRATLELLKELLAQQGVTIRYGLPRQKLTSIERDVIDMEVMLRSAWLLVIKAVW
                     MADNRKHNPLEASMSKLRAGDVVNKITQKAVEIMGPLGYSRDYLLEKWFRDAKITDIY
                     EGTGQINRLIVARNVLDYSGRQLR"
     gene            complement(1869..>2300)
                     /locus_tag="AC16V7_12830"
     CDS             complement(1869..>2300)
                     /locus_tag="AC16V7_12830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6258050.1"
                     /translation="LGGPQGHDFAPILSASRVALAAMAVGLSRAALEYACDYAKDRDV
                     FGVKVAQKQVIAFMIAEMATEVEAIKLLNWEAAWMLDTNKEDAAKNAYLAHTGAIDMA
                     MMVTDRAVQILGGHGYIREHPVELWMRNGRGISMLTGLAIV"
BASE COUNT          497 a          686 c          577 g          540 t
ORIGIN      
        1 atccgcatag cgtgtggtgt agtccagcgt ggcatagacg gcgggatcaa tgtaaccatg
       61 caaagcgatc accagcggta gtttttctcc agcctcaact gagacccggc gtggagtatt
      121 catgaagcca taaatacgta gcgcatcact ggggtacgaa accagggtgc gtgtgaagta
      181 actgtttgcc gccatggcct cttcaaccct cagctcgccc ccaccatagc cccgatttgc
      241 aaggtaatcg atgctgtatt ctagataggg gtcgggtgta gatgtgctgg tagaagtcgc
      301 cgtgatcgtc ggctctggtg ttaccgtcga agtaggtgta gccgttatcg ttatgatgtt
      361 aggcgttggc agcacgacct gagtgaccgt tgctaccggc gtgaattcgg gcgtcctttc
      421 aggcgccgag caacccgcga ttaacacggc agacaacaac aaaactagga tgttaaggtg
      481 ctttgtccga tggtcattca tcttgtacca ataccaataa gcaatatttg aattttaggg
      541 tgctgtttgc cgggtaagag catgcagtta cccgacctga gcactagcat ttagcgcaat
      601 tgtctgccac tgtaatccaa tacattgcgg gctacgatca agcggttgat ctgcccggtg
      661 ccctcgtaga tatctgtaat cttggcatcg cggaaccact tctccagcag gtaatcccgg
      721 ctatatccca gtggtcccat gatctctact gccttctggg tgattttatt gaccacatca
      781 ccagcgcgca gcttgctcat ggaggcttct agaggattat gtttgcgatt atcagccatc
      841 cacaccgcct taatcaccaa cagccaggct gagcgcagca tgacctccat atcgatcaca
      901 tcgcgctcga tgctggtcag cttctgccgg ggcagtccat agcgaatggt cacaccctgc
      961 tgggcgagca actcctttag caattctagc gtcgcacgtc caaccccaac acctgtcgcc
     1021 gccactaagg ggcgagtggc gtcaaaagtc gccatagcgc ctttaaagcc tttggtggtt
     1081 tccacctcca cctccgggct accaaggatg ttctcgaaag gcacgcggca atcctgcatc
     1141 acaatcgagg ctgtatcgct ggctcggatg cccattttgt gttccagctt ggtcacctgg
     1201 accccaggtg cgctcgcctc cactacaaag gggcgcatgc cggctcgccc agccttggga
     1261 tcgatggttg cccaaatcac aacgaagcca ttgctctcac ataacgactt atgcccagcg
     1321 gtgacaaaga tcttctcccc gttgagcacc cactcgttgg tagatttgtc cagcacggca
     1381 gtactgcgga tggcagaagt atccgagcca gcctgtggct ctgtcatcgc catggctgcg
     1441 aaagtcggac gctcctcctt gaagcgagct aaaaatttct ctttttgctc tggagtcccg
     1501 gtcgcctcca cagcggctgc acccaggcct ccccctggcg tcaccaagta tatgccgaca
     1561 tctccccagg ccaaaatctc tagcaaatgc gccaacgact ggtagccaat aggtgggcga
     1621 tccttcttcg gctcgtcacc atttttcttc ctctcgcgcg gcgtcagcga gcccgcgccc
     1681 gtagcgcgca tagccatatg catgaagttg atgtaatccc agggaatctc atgttcatgc
     1741 tcgtcgaaat agcgcgatac cgggcgcatc atgttttctg caacagtttt caatgcaaca
     1801 ctctgttgtg caatgggttt cggtgtttcg aaatcgatca ttttcaacct ccgtaatcta
     1861 tccagcccct acactatcgc caatccggtg agcatggaaa taccacgccc attgcgcatc
     1921 cacaactcca ccgggtgctc tcggatgtag ccgtgcccgc ccagaatttg aacggcacgg
     1981 tcggtaacca tcatcgccat atcaatggcg ccggtatgag ccaggtaagc gttcttagca
     2041 gcgtcttcct tgttggtatc cagcatccag gcggcctccc agttcaacag tttgatcgct
     2101 tcgacctccg ttgccatctc ggcgatcatg aacgcaatta cctgcttctg cgccaccttc
     2161 acgccaaaca catcgcggtc cttggcgtaa tcgcaggcat attccagggc agcccgagac
     2221 aagcccaccg ccatcgccgc caaggctact cgggaggctg acaggatagg tgcgaaatcg
     2281 tgcccttgcg ggccacccag
//
LOCUS       DBNHOH010000735         6638 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_172580, whole genome shotgun sequence.
ACCESSION   DBNHOH010000735 DBNHOH010000000
VERSION     DBNHOH010000735.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6638)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6638)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6638
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_172580"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="AC16V7_12835"
     CDS             complement(<1..659)
                     /locus_tag="AC16V7_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6258051.1"
                     /translation="MTTLVHEFRATYAFIERNANLVKRYWSWELVWMIYSIANSLSVS
                     FIGLGMERIGGGQNVDGRYLVLYLVIGTLVWRYLSLIFYWITEVISIERWEGTIEYTL
                     MAPIRRITHMAGQTFFAVIYSLLFTAVILVVTAVLFQLDLSDANLLGGTLMLLAGSLS
                     FIGVGVMGSILPLLFPERGAQMTHVIIALLLLVSGVYYPIEVLPLALQRLAVFSPATY
                     VL"
     gene            complement(686..1285)
                     /locus_tag="AC16V7_12840"
     CDS             complement(686..1285)
                     /locus_tag="AC16V7_12840"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6258052.1"
                     /translation="MEDIFHGELVRLGSDNLQTIAEALSRWHRDSEYARLLDDEPARL
                     KSPERIKEWLEKDLEEGQHSHFFFTIRTLEEDKLIGFIGLFVTQWSHGNSWVGIGLGE
                     REYWGKGYGTDAMRLILRYAFRELNLHRVSLGVFEYNPRAMRAYEKAGFQPEGRARKV
                     VYREGLRRDIIHMGILRDEWEELMRNIDASKNGNLLRFV"
     gene            complement(1242..1850)
                     /locus_tag="AC16V7_12845"
     CDS             complement(1242..1850)
                     /locus_tag="AC16V7_12845"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6258053.1"
                     /translation="MDIKTSLFTGEQICLAPIDPDKDAEIEAIWTQNAEYMRQLNLEP
                     ARPLSPEQVKKRYTEIEKEADEKKNLFYFTIRLRSDDQLIGFARIYWIGWTSGFGLVQ
                     LGIGDATQRGKGYGTQALSLLLRYAFAELNLHSLAADIPEYNLAATRLFQNAGFVEET
                     RQRQAVYREGRRWDLIYFGILKDEWSKRNGRHLSRRTGPSWQ"
     gene            complement(1879..2751)
                     /locus_tag="AC16V7_12850"
     CDS             complement(1879..2751)
                     /locus_tag="AC16V7_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6258054.1"
                     /translation="MYAIDLQDTYKHFGKSSQPLWKRWLWNQRGSNGGVGGSISNENG
                     SKNGSKSIVVAVDHVSFYVKEGEVFGVLGPNGSGKSTLIRLISTLLLPDAGRIVVFGH
                     DVVRQPIEVQRLINRVSVEASFFKKLSPMENLLYGARLYGMEGRETRQRVVEILSRLG
                     LEERTIYKPMEEMSRGMQQKVAIARALLSRPRLLLLDEPTTGLDPRSKREVQAVVREL
                     NQEYGTTILLTTHDMLEAENLCERIAIIDGGKVVALDSPEALKRTFPRNGHEPSLEDV
                     FLELTGKKLVRESD"
     gene            complement(3039..5354)
                     /locus_tag="AC16V7_12855"
     CDS             complement(3039..5354)
                     /locus_tag="AC16V7_12855"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6258055.1"
                     /translation="MSGNKDIFQKAMDEGHSAAWDQSWERAAGFYRQALEQFPEHHQA
                     LTSLGLALFELEDYEGALRAYAKAAMVSPDDPVPLEKISQSYEQMGNLERARAASLQA
                     AELHLKKRDVDKAIENWGRVTSFQPDNFTARSRLALVYERLGKANEAVAEYLVLAALF
                     QRANDLEKAMRAVQHAQKLKPGNTGALNALNLLNEQKPLPLPGHQREVAPQRRPRSRQ
                     LETSKASSAAQGLDPVTEARQKALSELAGVLFDIPDEEQGTSRRGFQTIVRGTGKLRS
                     RPVDYNRIVLYVGQVVEMQTQGDFAQAALELERAIDAGLDIPAAYYDLGFLQFHNQNL
                     ESATRNLQLALQHPDFVLAARLLLGQIYQRKGELREAAVEYLEALRQADTAIVPPDQA
                     DVLEQLYEPMVETQSQETNEQVQQQVCENISELLIRANWRDHLRQARQQLPVQTDGGP
                     PVPLAEIVVQARSSHIVESLTKIYQLERSGYLRSAIEEALFALEYAPTYLHLHVTIGE
                     LLLKLGEVEEAIEKFQVVAQAYSSRGEPRQALRIYRRVIELAPIELVPRERLIELLTS
                     MGRIEEALEEYMGFAEVHYSLADLEKARKTYVDAMRLAQKSQADRSMQIRILHRLADI
                     DLQSLDWREALRGFEQVRTLDPSDEEARSRIVEINFRLGQESRALAELDNYLEYLDGR
                     GKNDLARKFLEAQAKEEPQRVSLIRRLAERYHQAGQTSEAIAKLDEAGEILMQIGDVK
                     EAIQVVERILALRPPNAEEYQKLLRQLKSES"
     gene            complement(5341..>6638)
                     /gene="polA"
                     /locus_tag="AC16V7_12860"
     CDS             complement(5341..>6638)
                     /gene="polA"
                     /locus_tag="AC16V7_12860"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIR6258056.1"
                     /translation="PQLQAEMEARQANKLKNEVEMPLVEVLADMEMAGIALDTDFLAQ
                     MSAELSERMSAIEVQIFQQVGVSFNLNSPQQLSEVLFDRLKIQPPDRTQKTSSGFYST
                     AAGVLESLSGKHPVVDWVLEYRELSKLKSTYLDALPQQVNPRTGRVHTSYNQTGSVTG
                     RIASSDPNLQNIPIRTDLGRQVRQAFVAGAGMRLVSVDYSQVELRIVAHIAGDEAMLG
                     AFRSQQDIHVATAAAIYNVPIEAVDREQRRHAKAINFGLIYGMSAFGLTRTTDLTLAE
                     AEDFVAAYFKQFPGVKRYLDGVRRKAAEQEYVETLLGRRRYFPGLRNQSNRNIRNREE
                     REAINAPIQGTAADIMKIAMLGVPPALAEAGLSGRMLLQVHDELVLECPEEEVLLVAA
                     VVQKVMESAYRLSIPLATEARQGVNWGVMSPVRNSNERK"
BASE COUNT         1595 a         1877 c         1574 g         1592 t
ORIGIN      
        1 agcacatagg ttgccgggct aaaaactgcc aacctttgta gcgcgagcgg caagacttca
       61 atggggtagt agacgccaga taccagaagc agcaaagcga taatgacgtg ggtcatctgg
      121 gcgccccgct cggggaagag caggggcaag atagaaccca tcacacccac cccaatgaaa
      181 gacaggctgc ctgccagcag catcaatgtt ccaccaagca ggttggcatc ggacaggtca
      241 agctggaaca ataccgcggt caccaccagt atcaccgcag tgaacagcag gctatagatg
      301 acagcaaaga atgtctgtcc agccatgtgg gtgatgcgcc ggataggggc catcaacgtg
      361 tattcgatgg tcccttccca gcgctcaatg ctgatcacct cagtaatcca gtagaagatc
      421 aatgagagat agcgccatac cagcgtacca atcaccaggt aaagaaccag atatcgccca
      481 tctacatttt gcccaccgcc aattcgctcc atacccagac cgatgaagct aactgataag
      541 ctgttggcta ttgagtagat catccagacc agctcccaag accagtagcg tttgaccagg
      601 ttggcattgc gctctataaa ggcgtatgtt gctcggaatt cgtgcaccaa tgttgtcatc
      661 gattgtctcc taacatctaa ctcgatcaca caaagcgtaa taagttgcca tttttagaag
      721 catctatatt tcgcattaat tcttcccatt catcccgtaa aatgcccatg tggatgatat
      781 ccctgcgtaa gccttctcga taaacaacct tgcgggcgcg cccttcgggt tgaaatccag
      841 ccttctcata ggcacgcatg gcgcggggat tgtactcaaa gacgcctaag ctcacgcgat
      901 gtaagttcaa ctccctgaaa gcatagcgga gtataagtcg catagcatct gtaccataac
      961 ccttgcccca atactcgcgc tcacccaagc caattcccac ccacgagttg ccatggctcc
     1021 attgagtaac aaataaccca ataaatccga tgagcttatc ttcttcgaga gtgcgtatgg
     1081 tgaaaaagaa atgcgagtgc tgaccttcct cgagatcttt ttccagccat tccttgatgc
     1141 gctcaggtga tttcaagcgg gcaggctcgt cgtccagcag acgagcgtac tccgaatccc
     1201 gatgccagcg ggacaaagcc tctgcaatcg tctgaaggtt atcactgcca agacggacca
     1261 gttcgccgtg aaagatgtct tccatttctc ttactccatt cgtctttcaa aattccaaaa
     1321 tatattaaat cccatcgccg accttccctg tacacagcct ggcgctgacg cgtttcctct
     1381 acaaatccag cattttggaa caggcgtgtg gctgccaggt tatattcagg aatgtcggct
     1441 gccaggctat gcagattcag ctctgcaaat gcgtagcgta ataacaagct caaagcctga
     1501 gtcccataac ccttgcctcg ctgagttgca tcgccgatgc ctaactgaac caagccgaag
     1561 ccgctcgtcc acccaatcca atagatacgg gcaaacccga tcagctggtc atctgaacga
     1621 agccggatgg taaagtaaaa caggttcttt ttctcatcgg cttctttttc aatctctgtg
     1681 taacgtttct taacctgttc gggggacaag ggacgcgcag gctcaagatt cagctggcgc
     1741 atgtactccg cattctgggt ccatatagcc tcgatctccg cgtccttatc cggatcgata
     1801 ggggcaagac atatctgctc gcccgtgaat agtgatgtct tgatatccat tgttcatact
     1861 ccaatgacct gaaaatgatt aatcactttc tctaactaat ttcttgccgg taagctcgag
     1921 gaacacatcc tccaacgaag gctcgtgtcc atttcgaggg aaggtgcgct tcaaagcttc
     1981 aggtgaatct agcgcgacaa ccttcccgcc atcaatgatg gcgatacgct cacacaagtt
     2041 ttcggcttcg agcatgtcgt gcgtagtcag caagatggtc gtaccatatt cctgattgag
     2101 ctcgcgtaca actgcctgca cttcccgctt tgagcgagga tccaacccag tggtcggctc
     2161 gtcgagcagc aacaggcgcg gccgggatag taaggctcgg gcaattgcaa ccttttgctg
     2221 cataccgcgc gacatctctt ccatcggctt gtagatcgtc cgctcttcca gccccaagcg
     2281 tgacaggatt tctacaacac gctgtcgagt ctcacgccct tccatgccgt ataaccgagc
     2341 accataaagc aggttttcca tcggcgagag cttcttgaag aagctagctt ctaccgatac
     2401 acggttgatc aaccgctgaa cttctattgg ttggcgaacg acatcatgcc caaagacaac
     2461 gatgcgaccg gcgtctggca aaagcaatgt ggaaatcagg cgtatcaggg tagattttcc
     2521 agaaccattt ggtccaagaa cgccaaaaac ttcgccttcc ttcacataaa aagagacatg
     2581 gtccaccgcc acgacaatcg acttcgagcc atttttcgaa ccgttttcat ttgatatcga
     2641 ccctccaacc ccaccgttcg agcctctttg attccacagc cagcgcttcc aaaggggttg
     2701 ggaggacttc ccgaagtgtt tgtaggtatc ttgtaaatct attgcgtaca ttgctcttac
     2761 ctccacagaa aaaaattggc cacgggtacg cccgtggcca gcgaaagcca agaaaaaagc
     2821 cacggggctg gagagcaccc gtggccagtt actcaagata agattgaccg aatgatcaac
     2881 aggcgcactc caagaaggca caaccaaccg catcgccgat aggtgaagcg ttgcgcagga
     2941 caaaaatctt tgtgcatatg tctttcatga aatgcgctac tccttagatt aaagctttat
     3001 tagaacgaac cgagtttacc acactagtca aagtcgtgtc aagactcgct tttcaattgt
     3061 cgaagtaatt tctggtattc ctctgcattc ggcgggcgta gcgccagtat tctctcaaca
     3121 acctggatag cttcttttac atcgccaatc tgcatcaata tctcgcctgc ctcatccagc
     3181 ttggcgatcg cttcgcttgt ttgccctgcc tgatggtaac gctcggcgag gcgtcggatc
     3241 agacttactc gctggggttc ttctttagct tgcgcttcca agaactttcg cgccaaatcg
     3301 ttctttcccc tcccatccag atactccagg tagttatcca gttcagccaa ggcacgcgat
     3361 tcttgcccaa ggcgaaaatt aatctccacg atgcgggagc gagcttcctc atcagaggga
     3421 tccagtgtgc gaacctgctc aaagccgcgc aatgcctccc tccagtctag gctttgcagg
     3481 tcgatatcag ctaggcgatg caggatacgt atctgcatgg agcggtccgc ctgggacttt
     3541 tgtgcaagtc gcatggcatc aacatacgtt tttcgcgctt tttccagatc tgccaagctg
     3601 taatgtactt cggcaaatcc catgtattct tctaaggcct cttcgatccg tcccatggaa
     3661 gtgagcaact cgatcaaccg ctctcgtgga acaagctcta tgggagctaa ctctatcacc
     3721 cgccggtata tccgcagcgc ctggcgtggt tcaccccgcg aactatatgc ctgcgctacc
     3781 acctggaact tctcaatggc ttcttctacc tcccccagct tcagtaacag ctcaccaatg
     3841 gtaacgtgca ggtgaagata tgtgggtgca tactccaagg caaacaaggc ttcctcaatc
     3901 gcggagcgta aatatccaga acgttccaat tggtaaatct tggtcaacga ttcaacgata
     3961 tggctacttc ttgcttggac cactatctca gccagcggaa ctggcgggcc tccatcagtt
     4021 tgcaccggaa gctgctggcg cgcctggcga aggtgatccc gccagtttgc ccgtatcagc
     4081 aattcggaga tattttcgca gacttgttgt tgaacctgct cattggtttc ttggctctgt
     4141 gtttcaacca tcggctcgta gagctgctcc aaaacgtcag cctggtctgg cgggacaatt
     4201 gccgtgtcgg cctggcggag cgcttcaaga tactccacag ccgcctcgcg taattcacct
     4261 tttctctgat aaatctgccc taaaagcaaa cgcgctgcca acacaaaatc aggatgttgt
     4321 aaagcaagtt gaaggttgcg cgtagcactt tccagatttt gattatggaa ttgcagaaag
     4381 cctaggtcat aatatgctgc ggggatatct agtccagcat cgatagctcg ttccagttcc
     4441 aaggcagcct gcgcaaaatc accttgtgtc tgcatctcta ccacctgtcc cacgtagagc
     4501 acaatacgat tgtagtctac aggtctactg cgcagcttac ctgtcccgcg cacaatcgtc
     4561 tggaaaccgc ggcgggaggt gccttgctcc tcatcgggga tatcgaaaag aacacctgcc
     4621 agctctgata aggctttttg ccgcgcttcg gttacaggat cgagtccttg agcagcggac
     4681 gatgccttgg aagtttcaag ctggcgcgag cgtggccgcc gctgtggcgc aacttctcgc
     4741 tggtgacccg gtaatggcag tggcttttgt tcattcaaca aatttaacgc gtttagcgcc
     4801 ccggtatttc ccggcttgag cttctgggca tgttgtacag ctcgcatggc tttttctaaa
     4861 tcattggcgc gctgaaacaa cgctgccaac acaagatact ccgccactgc ctcatttgct
     4921 ttacccaggc gctcatagac cagtgccaat cgtgaacgcg ctgtaaagtt gtcgggctgg
     4981 aaactcgtga cacgccccca gttctctatg gctttatcga catcccgctt cttcaggtgc
     5041 aattctgcgg cctggagaga agcagcgcgg gcgcgctcca aattgcccat ttgctcatat
     5101 gattgagata ttttctctaa cggtacgggg tcatccggtg agaccatagc agccttggca
     5161 taagctcgca aagcaccctc gtaatcttca agctcaaata aagccaggcc aagactggta
     5221 agggcttggt gatgctcagg gaactgctct aaagcctgcc gataaaatcc cgccgcccgt
     5281 tcccaggatt gatcccacgc cgcggaatgg ccttcgtcca tagctttttg gaagatgtct
     5341 ttatttccgc tcattcgaat tcctcaccgg gctcataacg ccccaattca ctccctggcg
     5401 ggcttcggtt gccaggggga tactcaaacg ataagcactt tccatgactt tctgcacaac
     5461 tgctgcaacc agcaatactt cctcctcagg gcactccagc accagttcat cgtgtacctg
     5521 tagcagcatg cgacctgaaa ggccagcctc cgcaagcgcc ggaggcacgc ccaacatggc
     5581 aatcttcatg atgtcggcgg cggtgccttg gataggggca ttaatcgctt cccgttcttc
     5641 tcggttgcga atattgcgat tgctctgatt tctcaatccc gggaagtagc ggcggcggcc
     5701 cagcaaggtt tccacatatt cttgctcggc agccttacgg cgaacaccgt ccagatagcg
     5761 tttcaccccg gggaattgct tgaaataagc agcgacaaaa tcctctgcct cggctaaggt
     5821 caggtcagtg gtacgcgtta aaccaaaggc gctcattcca taaattaacc caaagttgat
     5881 ggctttggca tggcggcgct gctcacggtc gactgcttca atgggaacat tgtatatagc
     5941 cgccgctgta gccacatgaa tatcctgctg tgatcggaaa gctcctagca tggcctcatc
     6001 gccagcaatg tgcgccacga tacgcaactc cacttgcgaa taatcgacag acaccaggcg
     6061 cattcccgca ccagccacaa aagcctggcg cacctgccgc cccaggtctg tgcggatggg
     6121 aatgttctgt aaattaggat ccgatgatgc gatgcgtcca gtcacagatc cggtctgatt
     6181 ataggaggta tgcacgcgac ccgtacgcgg gttgacctgc tgaggcaggg catccagata
     6241 ggtggatttc agcttggaaa gctctctgta ttcgagcacc caatccacga ccgggtgctt
     6301 tccactcaaa gactcgagca cgcccgccgc agtagaataa aacccgctgg aggtcttctg
     6361 agtgcggtcg gggggctgga ttttgaggcg atcaaagagc acctcagaga gctgctgcgg
     6421 cgaattcaga ttaaaggaaa cacccacttg ctgaaagatc tgcacttcaa tcgcgctcat
     6481 gcgctcactc aattcggctg acatctgtgc cagaaaatct gtatccaggg caatgccagc
     6541 catctccata tccgctaaca cttccaccaa aggcatttct acttcgtttt taagcttgtt
     6601 cgcctggcgc gcttccatct cagcctggag ttgcggca
//
LOCUS       DBNHOH010000736         2732 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_109039, whole genome shotgun sequence.
ACCESSION   DBNHOH010000736 DBNHOH010000000
VERSION     DBNHOH010000736.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_109039"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2732)
                     /locus_tag="AC16V7_12865"
     CDS             complement(<1..>2732)
                     /locus_tag="AC16V7_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR6258057.1"
                     /translation="TFHRRIYGPHRLKGPLMRKGWKEWADAGFPDLDAATKSKYKFDS
                     RGLDELLPVSWETAYDYIARGMVAIAKRYSGEAGAALLREQGYPEEMIAAMGGAGTRT
                     LKCRGGMGLLGVIGKYGMYRFANTLALVDEHVRGVGEDEAKGGRTWSNYTWHGDQAPG
                     HPFVTGLQASDEDFNDLRNSRLHIQCGKNLVENKMPESHFFIEAMERGAKIVTITPEY
                     SPPATKSDYWIPIRPATDTALFLGISRWLMDNKKYDEAFVKRFTDLPLLVRADTLERL
                     RASDLFAGYQLGLNPDGPSFKLQGLKQDQYEKLGDYVVFDNKTSAPVAITRDDVGEAI
                     DNKGIDPALEWSGELRLVDGTQVEVLTIWSMYVEHLKDYDLDAVAEITHAPKAMIERL
                     ANDIATLSPVAIHIGEGINHWFHATLANRVQFLPLMLTGNIGRPGAGCYTWAGNYKAA
                     LFQGSEQTGPGFKGWVAEDPFHPNLDPKEHGKKIVAHSFTKDEEPAYWNHSERPLIVE
                     TPKYGRKVFTGNTHMPTPTKVQFFTNVNLLNNAKHHYEMIKNVNPNIELIISMDIEMT
                     STVEYADFGLAANSWAEFQVHEITASCSNPFLQIWKGGIPPLYDTRDDVRIVAEVAAT
                     LGDQFGDPRFRDYWKFALEGKPEVYIQRLLDSSLTTSGYRFEDIVAGRYGEPGAALML
                     FRTYPRIPFWEQLNDSVPFYTDTGRMNAYCDIPEAIEYGENIISHRESPEATPYLPNV
                     IVTSSRFVRPDDYGIPLDAEGWDERTVRNVSMAWEKVKDTKNPLWEAGYQFYCFTPKT
                     RHRVHSSWSTVDWTIILDSNFGDPYRMDKRLPGPGDHQLHMNPQAAKDMGLEDGDYVY
                     VDANPADRPYVGWKPDDPFYKVARLMLRLKYNPAYPYHIVMLKHGPFMATEKSV"
BASE COUNT          563 a          764 c          787 g          618 t
ORIGIN      
        1 caccgatttc tcggttgcca tgaaaggtcc gtgcttcagc atgacgatgt gataggggta
       61 ggctgggttg tacttcaggc gaagcatcag ccgggcgact ttgtaaaatg ggtcgtctgg
      121 tttccagccc acatatgggc ggtcggcggg attagcgtcc acatacacgt agtcgccgtc
      181 ttccaggccc atatctttgg ccgcctgggg attcatgtga agctggtgat cgcctggacc
      241 aggcaggcgt ttgtccatgc ggtaggggtc gccaaagttc gaatcaagga tgatggtcca
      301 gtccaccgtg ctccaagaag agtgcactcg atggcgcgtc ttgggtgtga agcagtagaa
      361 ctggtagccg gcttcccaca ggggattttt ggtgtccttt actttctccc aagccatgga
      421 tacattgcgc accgtgcgct catcccagcc ttctgcatcg agcgggatgc cataatcatc
      481 gggacgcaca aaccggctgg aggtgacgat gacgttcggc aggtagggag tggcctccgg
      541 gctttcgcga tggctgatga tattctcgcc atactcaatc gcttccggta tatcgcagta
      601 agcgttcatg cggcccgtgt cggtgtagaa cggcacggaa tcgttcaact gttcccagaa
      661 ggggatgcgc gggtaggtgc ggaagagcat taacgctgcg cccggctcgc cgtatcttcc
      721 agcgacgata tcctcaaagc ggtagccaga ggtggtcagc gatgagtcga gcagcctctg
      781 aatatacacc tcaggtttcc cctcgagggc gaatttccag taatctcgga agcgcgggtc
      841 gccaaactgg tcacccaggg tagccgcgac ctccgccacg atgcgcacat catcgcgtgt
      901 atcgtagagc ggtgggattc cgcccttcca gatttgcagg aagggattgg agcatgaagc
      961 ggtgatctca tgcacctgga actcagccca ggagttggcc gccaagccaa agtcggcgta
     1021 ctcaactgtg gaggtcatct caatgtccat cgagatgatc aactcaatgt tcgggttgac
     1081 gtttttgatc atctcataat ggtgcttggc attgtttaac aggttgacat tggtgaagaa
     1141 ctgtaccttg gttggcgtgg gcatgtgggt attaccggtg aaaactttgc gaccgtactt
     1201 gggggtctcg acgatcaggg ggcgctccga gtggttccag taagccggct cctcatcctt
     1261 ggtgaaggaa tgcgccacga ttttcttgcc gtgctctttc ggatccagat ttggatggaa
     1321 ggggtcttca gctacccagc ccttgaagcc aggtccggtc tgctctgagc cctggaagag
     1381 agccgccttg taattgccag cccaggtgta acagcctgca ccgggtctgc cgatattgcc
     1441 cgtgagcatg agcggcagaa actgcacccg gttagccagg gtggcatgga accagtggtt
     1501 aataccctca ccgatgtgaa tggcgaccgg gctgagtgtg gcgatgtcgt ttgcaaggcg
     1561 ctcgatcatc gctttgggcg catgggtgat ttccgccacc gcatccagat cgtaatcctt
     1621 gagatgctcc acgtacatag accaaatcgt cagcacctct acctgggtcc catcgaccag
     1681 cctgagctcc ccagaccact ccagggcagg gtctatccct ttattatcaa tcgcctctcc
     1741 cacatcatcc ctggtgatgg cgacgggggc tgaggtttta ttatcgaaga ccacataatc
     1801 acccaatttt tcgtactggt cctgcttgag cccttgcagc ttaaagctcg gcccatcggg
     1861 gttcagccca agctggtaac cggcgaagag atcgctggcg cgcaagcgct cgagggtatc
     1921 agcccgtacc aggagaggca aatcggtaaa tcgcttgaca aatgcttcgt cgtacttctt
     1981 gttatccatc agccagcggg agattcccaa gaacagcgca gtgtcagtcg caggtcggat
     2041 ggggatccag taatccgact ttgttgctgg gggcgaatac tccggcgtga tcgtgacgat
     2101 cttggcaccg cgctccatcg cctctataaa gaagtgagat tcaggcatct tattctcgac
     2161 caggttcttg ccacactgga tgtgcaggcg cgagttacgc aggtcattga aatcctcgtc
     2221 cgaagcctgc aagcccgtga cgaagggatg cccgggggct tggtcgccat gccaggtgta
     2281 attagaccaa gtacgcccac ccttggcttc atcttcgcca acgccgcgca cgtgctcatc
     2341 caccagagcc agcgtgttgg caaagcggta catgccatat ttgccgatta cgcctaacaa
     2401 tcccatgccg ccgcggcatt tcaaagtacg cgttcccgcg ccgcccattg ccgcaatcat
     2461 ctcttcaggg tagccctgct cccgcaaaag cgcagcgcct gcctcaccag agtagcgctt
     2521 ggcgatggcg accatgccac gggcgatgta atcgtaagct gtctcccacg agaccggcag
     2581 cagctcatcc agcccgcgcg aatcaaattt gtatttggac tttgtggcag cgtccaaatc
     2641 agggaagcca gcgtcggccc actccttcca ccccttgcgc atcagagggc ctttgaggcg
     2701 gtgcggtccg tagatgcggc gatgaaaagt ct
//
LOCUS       DBNHOH010000737         1779 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1134483, whole genome shotgun sequence.
ACCESSION   DBNHOH010000737 DBNHOH010000000
VERSION     DBNHOH010000737.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1134483"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1779
                     /locus_tag="AC16V7_12870"
     CDS             <1..>1779
                     /locus_tag="AC16V7_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR6258058.1"
                     /translation="TGSHPTQQLEPGSSLGNRYLIQDVIGLGGMGAVYRARDLHFPNV
                     EKLVAVKEMINQARDALVRSTIVRNFEREANLLATLDHPSIPRIYDYFTHNERSYLIL
                     EFIKGKDLEAILGETDGFVPQEKVIYWAVELCDVLHYLHSHKPEPIIFRDMKPSNVMI
                     NHHNHVILIDFGIAKPFQGSQRGTMIGTEGYSPPEQYRGEADHLADIYALGATLHHLL
                     TKIDPRLEAPFSFGERPLRQFNPNLSPELEGVVYKALQYAPQERYQSALEMKEALLIF
                     IKNTGILPPKPDKKSVPGAQEQEFQPMWEFACEDEIRGSATHANGAIYIGSYDNNLYA
                     LKATSGEFLWKYPADGGIVSKPLVYEGNIYFGSEDHRLHVINARHGQINWTYFTNGPV
                     RSSPYIAEGHVFIGSDDGYMHAVNTLSGRPAWKLDVGAAVRSTPIVEQEYVYFGTEAG
                     ELFCVDFSSKVRWRFKAKRAITSSPIVQNGVVYFTSMDSTLYAVDAKTGWVIWRFRLG
                     KASISTPCIAEDYIFTGAADGNIHCVDMRTAKQVWSFATEHQVTGSPIIYNDSLYCGS
                     VDKHVYCLEYSTGRLKWKYPTNGPITG"
BASE COUNT          464 a          464 c          420 g          431 t
ORIGIN      
        1 accggcagtc accctaccca acaacttgaa cccggctcat cgctgggaaa tcgctatttg
       61 atccaggatg tgatcggttt gggaggcatg ggagcagttt accgcgcccg tgacctgcac
      121 tttcccaatg tagagaaatt agttgcagta aaggaaatga tcaatcaggc gcgtgatgct
      181 ctggtccgca gcactatagt gcgcaatttt gagcgagagg ctaatctgct cgccacccta
      241 gaccatccaa gtatccctag aatctacgac tactttactc ataacgagcg ctcttatcta
      301 attctcgaat ttatcaaggg gaaagacttg gaagccatcc taggcgaaac ggatggtttt
      361 gtcccgcagg aaaaggtcat ctattgggct gtagaattat gcgatgtttt gcactaccta
      421 catagccata agcctgagcc aatcatcttt cgggacatga aaccctccaa tgtgatgatc
      481 aaccatcaca accatgtcat cctgatcgat tttggcatcg ctaagccttt ccaagggagt
      541 cagcggggca ccatgatcgg cactgaaggc tattcaccgc ccgaacagta ccggggtgaa
      601 gccgaccacc tagcagacat ttatgcattg ggtgccacgc tccaccatct tctgactaaa
      661 atcgatcctc gccttgaagc gccattttcc ttcggtgaac gcccgctccg gcaatttaac
      721 cccaacctct ctccagagct ggagggtgtg gtttacaaag cattgcaata tgcgccgcaa
      781 gagcgctatc agagcgccct ggaaatgaag gaagctctgc ttatttttat taaaaacacc
      841 ggcatacttc ctccaaaacc cgacaaaaag tctgtgcctg gcgcccagga acaagaattt
      901 cagccgatgt gggaattcgc ctgtgaagac gagatccgcg gctcagcaac ccatgcaaat
      961 ggcgctatct acattggttc atacgacaac aacctatatg ccttgaaagc tacatctggc
     1021 gaatttctat ggaaataccc tgcagatgga ggcattgtca gcaaacccct agtttatgaa
     1081 ggcaatattt actttggctc tgaagaccac cgcttgcatg taatcaatgc ccgccacggg
     1141 caaatcaact ggacatactt caccaatggg ccagttcgct cctcgccata catcgccgaa
     1201 ggccatgttt ttatcggatc ggatgatggc tatatgcatg ccgtcaacac gttgagcggt
     1261 cggcctgctt ggaagctcga tgtgggtgcc gctgtgcgct cgactcccat tgtagagcaa
     1321 gaatatgtct actttggaac agaagcaggc gaactttttt gtgtggattt cagcagcaag
     1381 gtgcgttggc gatttaaagc gaaacgcgcc ataacctcct cgcccattgt gcaaaatggt
     1441 gtagtttact tcacatccat ggactcaacc ctgtacgcag tggatgccaa gactggctgg
     1501 gtgatttggc gtttccgctt agggaaagca tccatatcaa ccccttgcat tgctgaagat
     1561 tacatcttta ccggcgcagc agatggaaat atccactgcg tggacatgcg cacagccaag
     1621 caagtatgga gttttgcaac ggagcatcag gtcactggct ctcccatcat ctataacgat
     1681 tcgttgtatt gtggatcagt tgacaagcat gtttattgcc ttgaatacag cacaggtcgg
     1741 ctaaagtgga agtaccccac aaacggacca attaccgga
//
LOCUS       DBNHOH010000738         2440 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_686565, whole genome shotgun sequence.
ACCESSION   DBNHOH010000738 DBNHOH010000000
VERSION     DBNHOH010000738.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_686565"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="AC16V7_12875"
     CDS             <1..719
                     /locus_tag="AC16V7_12875"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HIR6258059.1"
                     /translation="PHFDDVAISCGGLAWEQAQRGNRVEVWTICAGEIPPGAISPYAE
                     SLHKRWQTSQNTPAQRRDEDLASCAAMGASAVHFHIPDSIYRRGGTDNIPLYDSENDI
                     FGSLHPNDSHTVEKIRGELKHRLPEDAELVSPMTIGGHVDHRLVRAGAEQLGRRLWYY
                     ADAPYVMKHPGELDKLIQDDWIRQVFPISDAGLAAWQGAVAAHASQISTYWPNLEAMR
                     SVISNYCIENEGVRLWRAPA"
     gene            872..1990
                     /locus_tag="AC16V7_12880"
     CDS             872..1990
                     /locus_tag="AC16V7_12880"
                     /inference="COORDINATES: protein motif:HMM:NF024491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HIR6258060.1"
                     /translation="MSSKRKSRRAYRSPQTRVGSLLAFIVLTLAFFLYRSVSNSSPPV
                     ATPLPPAPGSSSSEWFSVYFTDPSGPGAESLRGGPDSALAAAIDEARLSVDIAAYDFD
                     LWSLRDALMAAQRRGVTVRMVTDSDYIENDEVQELRDAGIQVLGDRREGLMHDKFVII
                     DRLEVWGGSMNYTVNDAYKNNNNLIRIRSSRLADNYTTEFEEMFVDDQFGPRSPANTP
                     YPSLSVEGTTIEIYFSPDDGVADQLTDLIQSAQQSIHFLAYSYTSDDIADAMLERARA
                     GIQVAGVFEENQVKSNRGDEYSRLRKAGLDVRLDGNPRNMHHKVILIDGQIVVTGSYN
                     FSASAENSNDENTLVIFNSKIAAQFEDEFARVFAQAQP"
     gene            2033..>2440
                     /locus_tag="AC16V7_12885"
     CDS             2033..>2440
                     /locus_tag="AC16V7_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA domain-containing protein"
                     /protein_id="HIR6258061.1"
                     /translation="MDNSNFYLGRLFDTQHRKPTKSPLLYDSADLTTHAVVVGMTGSG
                     KTGLCIDLLEEAALQGIPALMIDPKGDITNTLLHFPDLLPQDFQPWVNADQARREGKT
                     PEQAAEEASERWRQELNAWEIPVERVRALKNSAH"
BASE COUNT          562 a          706 c          634 g          538 t
ORIGIN      
        1 cccctcattt cgatgacgtt gccatctcct gcggcgggct tgcctgggaa caagcgcagc
       61 gcgggaaccg cgtcgaggta tggacaatct gcgctggaga gataccgccg ggagccatct
      121 caccctacgc tgagagcctt cataagcgct ggcagaccag ccaaaataca cccgctcaac
      181 gccgtgatga ggatttggct tcctgcgcag cgatgggcgc atcggcagtt cacttccata
      241 tccctgattc catttatcgc cgcggtggaa cggataacat cccactgtac gattctgaaa
      301 acgacatttt tggctccttg catccgaatg actcccacac tgtggagaaa attaggggag
      361 agctcaagca ccgcttgcca gaggacgccg agttggtctc cccgatgact atcggcgggc
      421 acgtcgatca tcgcctggtg agagccgggg cagagcaatt gggaaggcga ttgtggtact
      481 acgcggatgc cccgtacgtg atgaaacacc ctggcgaact tgacaaattg atccaagacg
      541 attggatccg ccaggttttc cccatctcag atgccggcct ggcagcttgg cagggggccg
      601 tagctgccca tgcatcacag atcagcacgt actggcctaa tttggaggcc atgcgctccg
      661 tgatcagcaa ctattgcatc gaaaatgagg gtgtccgcct ctggcgagcc ccagcataat
      721 ctcatattcc tcacccccta tacttcccct cggttacaga gattcccaca caccccataa
      781 ttgatatgtc cttgggctgt gattggcatg ctataatgtc tggcgaaatt agtcttgttt
      841 agacagattt cgccgcttca ctaaggagtt tatgtcttct aaacggaaat ctcgtcgcgc
      901 ataccgctca ccccaaaccc gagtgggtag cctgctggcc ttcattgtgc tgactctggc
      961 ttttttcctt tatcgttcgg tcagcaattc gagtccgccg gtcgctaccc cgcttcctcc
     1021 tgctccaggc tcatcatcct cggagtggtt cagcgtctat ttcacagatc ccagcggtcc
     1081 aggcgccgaa tcattgcgcg gtgggccgga ttctgccctg gctgccgcca ttgacgaagc
     1141 tcgcttgagc gtggatatcg cagcctatga tttcgacctg tggagcctgc gtgatgcact
     1201 catggctgcc cagcgccgag gcgtcaccgt gcgcatggtt accgacagcg actacattga
     1261 aaacgatgaa gtccaggagt taagggatgc aggcatccag gtcttgggtg atcgccgcga
     1321 ggggctgatg cacgataaat tcgtgatcat cgaccgcttg gaggtgtggg gcggctcaat
     1381 gaattacact gtcaatgatg cttataagaa taacaacaac ctcatccgca tccgctcatc
     1441 gcgtttggca gacaattaca caaccgaatt tgaagagatg ttcgttgacg accagttcgg
     1501 gccgcgatcg ccggccaaca ccccctaccc aagcttgagc gtcgagggaa ccacaatcga
     1561 aatctacttt tcacccgatg acggcgtcgc tgaccaactt accgacctca ttcaaagtgc
     1621 tcagcaaagc atccacttcc tggcatactc atatacatcc gatgatatcg ccgacgccat
     1681 gctagagcgc gcccgggcag gcatccaggt ggcaggcgta tttgaagaga atcaggtcaa
     1741 atccaatcgc ggtgacgaat acagccggtt gcgaaaagcc ggcctggacg tgcgcctgga
     1801 tggtaaccct cgcaacatgc accataaagt cattcttatc gacgggcaga tcgttgtcac
     1861 cggatcatac aacttcagcg ccagcgccga aaacagcaat gacgaaaaca ccttggtgat
     1921 ctttaattcc aaaattgccg cacaatttga ggatgagttt gcaagagttt ttgcacaggc
     1981 acagccatag ctggataccc gtttagttcg tgttcacata tctgggatat ttatggacaa
     2041 ctctaatttc taccttggac gcctctttga cacacagcat cgcaagccga ccaaatcgcc
     2101 cctgctctac gactcggcag acctgaccac acacgctgtg gttgtgggta tgaccgggtc
     2161 tggtaagacc ggcctgtgca ttgacctgct ggaggaagcc gccttgcaag gcatccctgc
     2221 cttgatgatc gatcccaaag gcgacatcac caataccctg ctgcattttc ccgatttact
     2281 accgcaggat ttccagccct gggtgaatgc cgaccaggcg cgtcgcgagg gaaaaacccc
     2341 ggaacaggcc gctgaggaag cgtctgaacg ctggcgacag gagctgaatg catgggaaat
     2401 cccagttgag cgggtacgcg ccctgaaaaa cagcgcccac
//
LOCUS       DBNHOH010000739         4556 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_879039, whole genome shotgun sequence.
ACCESSION   DBNHOH010000739 DBNHOH010000000
VERSION     DBNHOH010000739.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4556)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_879039"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="AC16V7_12890"
     CDS             <1..930
                     /locus_tag="AC16V7_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR6258062.1"
                     /translation="IEGEEETSSVHLNKFVKDNAEKLAADACVWEFGQVDHREMPIQH
                     LGLRGICYVELSVETAEQDVHSGIGGSIFPNAAWRLVWALNTIKGPDERILIPGHYDN
                     VVAPSEQDEQYIAELPEVADEYKNRYGVQYFLHGLDGGVELRLAEVFQPTCTICGLTS
                     GYQGPGSKTVLPAQASAKVDFRLVPDQTPEQVLSGLRRHLDDQGFTDVKITYLGGESP
                     ARTDPNHSFVDMVVKTSQSVYDHPMQIIPITGGSGPIHAFIHYLKLPVVTAGLGYPGT
                     RAHAPNENIRLDLYLKHAKHVARIMTAFGDGTG"
     gene            1026..3737
                     /locus_tag="AC16V7_12895"
     CDS             1026..3737
                     /locus_tag="AC16V7_12895"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6258063.1"
                     /translation="MKLSLQRDLGLQLLALYLLFVGPVVVAALIFDRVASQRIESDVK
                     AADLALARAIAQETNTALGDALQSVENLASYQEVISMDKRGMEAIFTTLMSARPDVNL
                     VYRLDSQGLMVFHVPLGPGTTVGQDFSFRDYYQHALTARQPFLSQGRISPTTEQPVTT
                     AVMPIWDSHDNFLGLVATNIKLQSLSNTLSSIVAEHRPEEFFNVTIIDISGKVIAHPD
                     PQYPLTEAEFNLPEVTRAVLSGSSGNLIRPDPGGNNTLYSYVPIPSAGWGVIVSRPTA
                     AAFATSQATHRGVLLTIGVFLIVGLFFWLALSRQVLHPLERLAAFSRTIGLDEEFSED
                     HRLQLTTLSERPDQIGHLTRSLTRMEKAIEARLQELSTLLETSAAVVSTLDSNLVLNR
                     ILEQVERLMDIQMGAILALDEKRGVFRAQASRGLSKRYTENLAISPQEPISVTLRAIR
                     SGEPIQISDTEKDSSFTAMRPRARSEGYRSVLAVPLNIKHGPPSALLVYRPDPHIYTK
                     REINLLTSFANHAAMAIENAALYARSDMRLREQTRRLEALIQSLQDGLIMEDLQGIVL
                     HANRRIGEILELPLEEIIGVPGSQLIEHLLNHLASQDNAQRAKTTRLVEDALTGEGSR
                     RVELEIVTNHKLCYMVLRTFDVTDADGTPIGRGHILRDITRNREIDRMKSSLISTVSH
                     ELRTPLAAIKGYVTTLLAEDVMWDSLTQREFLEIISNESDRLSKLVNDLLDMSRIEAG
                     SLDVNRAQCNLEEIIQRAAQRAYPTPIERLVVNIPPDLPSLYVDPQRIEAVLRNLIEN
                     AAKYAGDISPVSISASVEGEEMVVRVRDEGPGIPAEHSNRIFESFYRLESNLTRMAHG
                     DGLGLAICQGFISAHGGKVWLEKQTKGTCVAFSLPLSQIPVEQLKAE"
     gene            3779..4468
                     /locus_tag="AC16V7_12900"
     CDS             3779..4468
                     /locus_tag="AC16V7_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6258064.1"
                     /translation="MVNITVLVVDDEKPLRDFVRRNLDVRGFNVRVASNGLEALAIFN
                     TQTIDLVILDVMMPRMDGLETIRRIRQSSLVPIIVLSALGEESDKIRALNLGADDYLT
                     KPFGVGELLARVQAVLRRARWAEPPTRQGRLARGEVVIDLERHETTVQGKEIELTPTE
                     FNLLVYLMENSGKVLPHQTILRHVWGPEYGPEHEYLRVYIGRLRQKIEDDPANPKYLI
                     TERGIGYSFEG"
BASE COUNT         1137 a         1229 c         1147 g         1043 t
ORIGIN      
        1 atcgagggtg aagaagagac cagcagcgtc catctcaata aatttgtcaa ggacaacgca
       61 gaaaagctcg ccgcagatgc ttgtgtatgg gagttcgggc aggtggacca tcgcgaaatg
      121 ccaatccagc accttggcct gcgcggaatt tgctatgttg agttatctgt ggaaacagcg
      181 gagcaggatg tccactcggg aattggcgga tcaattttcc cgaatgcagc ctggaggctg
      241 gtttgggcat tgaacaccat aaaagggcca gacgagcgca ttctcatccc tggtcactac
      301 gacaatgttg ttgcgcctag cgagcaagac gagcagtaca tagcagaact ccctgaagtg
      361 gcagacgaat acaagaatcg ttatggagta caatattttc tacacgggct ggatggcggt
      421 gttgaattgc gcctggcaga ggtatttcag cctacttgca ccatttgtgg actgacgtct
      481 gggtaccaag gaccaggcag taaaactgtt ttgccggccc aagcctcagc caaggtggat
      541 tttcgcctgg tgccagatca aaccccagag caggtgttat caggtttgcg cagacactta
      601 gacgaccaag gatttaccga tgtaaagata acctacctgg gtggggagtc ccctgcgcgc
      661 accgacccga accactcctt cgttgacatg gtagttaaaa cctcacaatc tgtctacgat
      721 cacccgatgc aaatcatccc gatcaccggt ggatccgggc caatccatgc ttttatccat
      781 tacctgaagc taccggtagt taccgcaggt ttgggctacc ctggtactcg agcgcacgcg
      841 cccaacgaga atatccggct agatctttat ctcaaacacg ccaagcacgt agcaagaatc
      901 atgacagcgt ttggcgacgg tactggatag tttatgcgag gatatgagcg gatcccatta
      961 cgtgatcctc accagcgaat tcaataatcc atgatattct ttttggaatt cggcgataga
     1021 tttcgatgaa actatctctg caacgtgacc tgggattgca attactggcg ctatatctct
     1081 tatttgtagg gccagtggtt gtggctgcgt taattttcga ccgcgtggct tctcaacgta
     1141 tcgaatctga tgtcaaagca gcagacctcg ccctggcgcg cgctatcgcc caagagacca
     1201 ataccgcttt aggtgacgct ctccagtcgg ttgagaacct ggcctcatat caagaagtaa
     1261 tatcgatgga caaaagaggc atggaagcca tctttaccac actgatgagc gcccgccctg
     1321 atgtcaacct agtataccgc ctggattcgc aaggcttgat ggtctttcat gtaccacttg
     1381 ggccaggcac aaccgtaggc caggatttct cgtttcgaga ttattaccag cacgctttga
     1441 cagcacgcca acccttcctc tcccaagggc gtatatcacc cacaacagaa caacccgtaa
     1501 ctaccgccgt catgccgatt tgggactcac atgataactt tctcgggctg gtggcaacca
     1561 atattaagct gcaatctcta agcaacaccc tatccagcat tgttgcagaa caccggccgg
     1621 aagaattctt caacgtgacg attatcgaca tcagcggtaa ggtgatcgcg caccccgacc
     1681 cacaataccc actcaccgaa gccgaattta acctacccga ggtaacccgg gcagtgctct
     1741 ccggctcttc cggcaatctg atcaggccgg acccaggtgg aaataatacc ctgtacagtt
     1801 atgtacccat cccaagcgcc ggatgggggg ttatcgtcag ccgccctact gctgctgcat
     1861 tcgctacctc gcaagctact caccggggtg tgctccttac catcggagtg ttcctgattg
     1921 ttggcttgtt tttctggtta gccctatccc gacaagtgct tcacccatta gaacgcctgg
     1981 ccgcgttcag ccggactatt ggtctggatg aggaattctc cgaggatcat cgcctccagc
     2041 taacaacact gtccgaacga ccagatcaga tcgggcacct cacacgcagc ctgacccgca
     2101 tggaaaaagc tatcgaagcg cgcctacaag agctctcgac attacttgaa accagcgcgg
     2161 cggtcgtatc caccctcgac tctaatctgg tccttaaccg gattctcgaa caagttgaac
     2221 gcctcatgga tatccagatg ggtgccattc tggctctgga tgaaaagcgt ggcgtcttcc
     2281 gcgctcaagc tagccgaggg ctctcaaagc gatacacaga gaatttagcc atctcccctc
     2341 aagaaccgat ttcagtaacc ctgcgagcca tccgtagcgg ggaacccatc caaatcagcg
     2401 acactgaaaa ggatagctcc ttcaccgcaa tgcgcccgcg cgcccgttca gaaggatatc
     2461 gctctgtctt agcggtgccg ttgaatatta agcacggacc accttctgca ctgctggttt
     2521 atcgccctga tccacatatc tacaccaagc gtgaaattaa tttgttgact agctttgcaa
     2581 atcatgctgc catggctatc gagaacgctg ccctctacgc acgcagcgat atgcgattac
     2641 gagaacaaac ccgtcgactt gaggcactga tccaatcgct gcaggatggt ttgatcatgg
     2701 aggaccttca agggattgtt cttcacgcca accgccggat cggggagata cttgaattgc
     2761 ccttggagga gatcatcggg gttccgggca gccaactcat cgagcatctg ttaaatcact
     2821 tagccagtca agataatgca cagcgggcaa aaacaacccg tttggtggaa gatgcactca
     2881 ccggagaagg ttcgcgccgt gttgaactgg aaatcgtgac caaccataag ctatgctata
     2941 tggtccttcg aacttttgat gtcaccgatg cggacggcac accaattggt cgtggccata
     3001 tcttgcgtga catcacacgt aaccgagaaa ttgaccgcat gaaatccagc ctgatctcca
     3061 ccgtgtccca tgaactgcgt acccccttgg ctgctattaa aggctatgtg actacactgc
     3121 ttgccgagga cgttatgtgg gatagcctga cacagcgtga attcctggag atcatctcaa
     3181 acgaaagcga tcgtctgagc aagctggtca acgacttact ggatatgtcg cgcatcgaag
     3241 caggcagtct ggatgtaaac cgagcgcagt gtaatctaga agaaataatc cagcgcgccg
     3301 ctcagagggc ctatccaacc ccaattgaac ggcttgtggt taacatccca cccgatctgc
     3361 cgtctctcta tgtcgatcca cagcgtattg aggctgtctt acgtaaccta atcgagaatg
     3421 ccgcaaaata tgcgggtgac atctctccgg taagcataag cgcttcggtg gaaggagaag
     3481 agatggtagt gcgtgttcgt gatgagggac ccggaatacc cgctgagcac agcaaccgga
     3541 tctttgagag cttttaccgc ttggaaagca atctgacacg catggcacat ggggatgggc
     3601 tgggtttggc aatttgccag ggattcatca gcgcgcatgg tggcaaagta tggcttgaga
     3661 aacagaccaa aggcacatgc gtcgcattct ctctaccact gtctcagata cccgtggagc
     3721 agctcaaggc tgaatgaccc gtagttgatt atccagccag ggaacaacct tctttgtcat
     3781 ggtcaacatt accgtcctag tcgtagatga tgagaaaccg ttgcgtgatt ttgtgcgccg
     3841 caatctggac gtgcgcggtt tcaatgtacg tgttgcctcc aacgggttgg aggcccttgc
     3901 catattcaac acccaaacta tcgacttggt aatcttagat gtgatgatgc cgcgcatgga
     3961 tggtctagag actatacggc gcatcaggca gagttcactg gttcccatca tcgtattgtc
     4021 tgccctgggt gaagagagcg ataaaatacg cgccttaaac ctgggcgctg acgactacct
     4081 gactaagcct tttggggttg gtgagttact ggcgcgcgtc caggccgtcc ttcgtcgcgc
     4141 ccgttgggca gaaccaccaa ctcgtcaagg tcgactggcg cgaggtgagg tcgtgatcga
     4201 tctagagcgc cacgaaacca ctgtacaggg taaagagatc gaattgacac ccactgaatt
     4261 caatttgctt gtttacttga tggagaattc cggcaaggta ttgccccatc aaaccattct
     4321 gcgacacgtt tggggaccgg aatacggccc ggagcacgaa tacctgcggg tgtatatcgg
     4381 caggttgcga caaaaaatcg aggacgatcc tgccaatccc aaatatttga ttaccgagcg
     4441 tggaataggc tacagcttcg aaggctgatc aaccagagac caaacctcag cgctcacaag
     4501 aggatttcca ccaccctccc cggtcgctta taggctgctt aataaccacg gtgagt
//
LOCUS       DBNHOH010000740         1106 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_301914, whole genome shotgun sequence.
ACCESSION   DBNHOH010000740 DBNHOH010000000
VERSION     DBNHOH010000740.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_301914"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..>1106)
                     /locus_tag="AC16V7_12905"
     CDS             complement(129..>1106)
                     /locus_tag="AC16V7_12905"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR6258065.1"
                     /translation="LSTPLNYPQANGLVELRQRVASLYPGATPDNVLITTGCAQANFT
                     SLLTIMAPGDEIVVMLPNYMQIWGIAQNFGYQVKTFSLKEELDWGIDIDELNAVVSSK
                     TKLIAIVNPNNPTGHIMTTDEMDAVVAVAQRSGAWLLCDEVYAGAERVTEQVTPSFWG
                     RYERVLATGGMSKAYGLPGLRIGWVIAPADTIEKIWAWQDYVTISTTMLANKLATYAL
                     SPQVRPRLLKRTREYIRNGYRNFELWRAKHTDLFSLVPPQAAAIAFVRYHLEVNSMEL
                     VKRLIQEQAFVLPGDVFGLDHFLRISYGLPADYLNEGLNRLYKVMSQTG"
BASE COUNT          250 a          294 c          299 g          263 t
ORIGIN      
        1 ttttggaaag aatgagggaa ttgggtagcc acgtccgccc aattccctca atacattatt
       61 gtgaggcttt gtcttaaacc ccccatttaa ttgtgggcag gctaaatcta ctatgaatga
      121 tggacatctc acccggtttg gctcatcact ttatacaggc ggtttagtcc ctcgttcagg
      181 taatccgctg gcaagccata gcttatccgc aggaagtggt ccagcccaaa cacatcgccg
      241 ggcaggacga atgcctgctc ttggatcagg cgtttaacca actccatcga gttaacctct
      301 aaatggtagc gaacgaaagc gatcgcggca gcttgaggag gaaccaggga gaataaatca
      361 gtatgcttgg cgcgccacag ctcgaagttg cggtatccgt tgcggatgta ttcccgcgtg
      421 cgctttaata ggcgcgggcg aacttgaggc gatagggcat aggtagccag tttgttggcg
      481 agcatggtgg tgctgatggt tacgtaatct tgccaggccc agatcttttc gatcgtatca
      541 gccggggcga ttacccaacc aatgcgcaag cctggcaatc cataggcttt cgacatgccg
      601 cctgtcgcca gcacgcgttc gtaccgcccc cagaaggaag gggtgacttg ctctgtaacc
      661 ctctccgctc cagcgtacac ctcgtcgcac aacaaccagg ctccgctgcg ttgagccacc
      721 gcgacgactg catccatctc atccgtggtc atgatgtgcc cggtgggatt attcgggttg
      781 acaatggcga tcagcttggt cttgctggac actacagcgt ttagttcatc gatatcaata
      841 ccccagtcca gctcttcttt cagtgagaac gtctttacct gatagccaaa attttgtgca
      901 atcccccaga tttgcatata gttgggcagc attaccacaa tctcatcacc gggtgccatg
      961 atcgttaaca gcgaggtgaa gttcgcctga gcgcaaccgg tggtgatcag tacattgtcg
     1021 ggcgtagcgc cggggtagag cgaagcgacc cgttggcgca actcaaccag gccattggcc
     1081 tgcggataat tcaggggtgt ggagag
//
LOCUS       DBNHOH010000741         7665 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_814456, whole genome shotgun sequence.
ACCESSION   DBNHOH010000741 DBNHOH010000000
VERSION     DBNHOH010000741.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7665)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7665)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7665
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_814456"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="AC16V7_12910"
     CDS             <1..953
                     /locus_tag="AC16V7_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258066.1"
                     /translation="QERSLGGMALILGSLALSMPLSPPSTALLLVILVLQAIAMGKAW
                     LPSGWREQRWFWPALVGAILLVGIGLWLALLQFAPAGVTSPISVVSWWIKKSADWNAY
                     LSERASGWVQKIFDSTPEWTHMPLLVLYGVIQPFLPAAVIDVTGAPVWRLIAIWRAAG
                     WTLLLPLLLYATLRALRPKSSLSASQGLSASGERVLSLAVWLVILVASFRGGGDAWDN
                     PRYRLTFAGLQMALVAWAWAEQRRLPDPWLRRVIIGLGLVLAWFVPWYLRRYLHMQWP
                     VADFFMTLGLGLASAALYWIGDWGLAKFKQRKQSDADHHE"
     gene            1216..2292
                     /gene="asd"
                     /locus_tag="AC16V7_12915"
     CDS             1216..2292
                     /gene="asd"
                     /locus_tag="AC16V7_12915"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012476745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIR6258067.1"
                     /translation="MNNQQSQPRERIPVAVLGATGAVGQRFVQLLDNHPWFKVVVLTG
                     SERAIGKTYAQACHWILSTPMPEWAREISILPAVPDDSDVPLVFSALPSSSAYEVEPY
                     YARRGVIVCSNASAFRAEPDVPLVLPEVNPEHISLISHQREKRGWTGAIVTNANCTIT
                     GLTIVLKALADQFGINKVFVVSMQAVSGAGYPGVSSMDILGNVVPHIYDEEDKIESEP
                     RKIMGTAKDDHVELADFEITAHANRVPVLDGHMVCLSIELANKGSIDVVAEALRGFQA
                     PPISRDLPSAPRSVIELKDEPDRPQPRLDIMTGRGMTTLVGRLREDPVLDYKMVILSH
                     NTIRGAAGASIYNAELLVKQGFFE"
     gene            2311..2550
                     /locus_tag="AC16V7_12920"
     CDS             2311..2550
                     /locus_tag="AC16V7_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HIR6258068.1"
                     /translation="MCRSIKTLRRPEQPPTEEELHAAALQYVRKVSGYRMPSRRNQVA
                     FEQAVEEIAMVTQKLLDSLVTTNSRLSGSKSMKGE"
     gene            2552..2770
                     /locus_tag="AC16V7_12925"
     CDS             2552..2770
                     /locus_tag="AC16V7_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HIR6258069.1"
                     /translation="MGESVYKVIELVGTSSESWEKAATAAVEMAAKSLRDLRVAEISV
                     LDMTLENGKVRLYRAKVKVSFKYEREED"
     gene            complement(2782..4329)
                     /gene="guaA"
                     /locus_tag="AC16V7_12930"
     CDS             complement(2782..4329)
                     /gene="guaA"
                     /locus_tag="AC16V7_12930"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR6258070.1"
                     /translation="MDTIAILDYGSQYAQLIARRVREAQVYCELFPWDAPPEQVLALQ
                     PKGFILSGGPASVYEPDAPTLLPYILESDLPVLGICYGMQVLTHALGGRVAPAAQREY
                     GPALVETLKPNPLLADGDHPVWMSHGDRIETPPAGFSILARSDNSPIAAMGDLQRGYF
                     GVQFHPEVHHTPGGAEILRRFALDACHARPEWTPESIITNSMARIRQQVGQEMVLSAV
                     SGGVDSSVATTLVSRAVGDRLVSVFVDTGLLRQGETAQVQAALHDALGAELVTVDAVD
                     QFMAALRGVTEPEQKRRIIGELFIRIFEQQARQLGMPRLLVQGTIYPDVVESSAPDRA
                     KAQRIKTHHNVGGLPAEMGFELVEPLRYLFKDEVRMVGEALGLPSSLIWRQPFPGPGL
                     AVRCLGEVTRERLERLRLADAILTEELAASGLLRMGRRGEGTAQAFAVLLPVQSVGVM
                     GDQRTYQETVALRAVTTEDFMTADWARLPYDLLARVANRIVNEVQGVNRVVYDITSKP
                     PATIEWE"
     gene            complement(4379..4954)
                     /gene="xpt"
                     /locus_tag="AC16V7_12935"
     CDS             complement(4379..4954)
                     /gene="xpt"
                     /locus_tag="AC16V7_12935"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HIR6258071.1"
                     /translation="MEALKERILREGKNLGGGILKVDGFINHQVDPELMEACGHELAR
                     LFRDVGASKVLTAEISGIAPALSTAMHLGLPVVYARKTKPITMPDQVYLTLSPSHTKG
                     RMVELIVSPEYLAGGERVLIIDDFLASGATILGLARLAQTAGAIIVGVGTLIEKTFEG
                     GRQVLAGLGVPIEALARIRAMEGDTILFEDE"
     gene            5106..5756
                     /locus_tag="AC16V7_12940"
     CDS             5106..5756
                     /locus_tag="AC16V7_12940"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR6258072.1"
                     /translation="MLVLASNSPRRRQLMALGGWTFTLIPAAVDERPLPGEPPREYVL
                     RLAEDKARTVAGRAPQDALVIAADTAVVDGADILGKPDDAQQAETMLRRLRGRSHQVY
                     TALAVLRLTDGVLLSDLCQTEVPMRHYSDEEMAIYIASGDPFDKAGAYAIQHEEFRPV
                     EHLSGCFANVMGLPLCHLTRTLKKLGDPPERDVPTACQAALQFNCSVYPQVLTGEL"
     gene            5762..>7665
                     /locus_tag="AC16V7_12945"
     CDS             5762..>7665
                     /locus_tag="AC16V7_12945"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR6258073.1"
                     /translation="MKFQHWLFILLIALLASCSTSPSVTTTPSLVPTRTLAQPGVGTT
                     PVPDPGSVASAFLDAWKADDYETMYALITPLSQDAMSQESFVKHYAGIAAEMALSGID
                     YEVLSSLVEDPSSAQVGYRVIFHSSLVGDIQRETLMNLSLDQGEWRVKWDDSLVMPEL
                     AGDNFMGMERYIPARANIYDRNGHALVAQTDVTAIGLVPNQIDPDQEEKLYSWLVRLT
                     GLTGDEIVSRYASFPPGVDWYLPLGEVAAETIASNIDILSGFSGLVLGPYRARYYFDG
                     GIAPHVVGYVTTIQAGQEEEYLRKGYQRDERVGQAGLEKWGEDYLSGVRGGALYVFNP
                     QGQIITRLAEREAQPSQAIYTTLDRDFQQEVERAISGYRGAVVVLERDTGRVLAIASS
                     PSFDPNAFEPRNRNSSAMLEDIFSDPSTPTFNRAAQGQYPPGSAFKPVTMAAALESGL
                     YTPETTYQCGYFFNELAGLTLRDWTYEHYLEDGKTIPSGLLTLPEGLMRSCNPFFWHM
                     GLDLYNQDKTTAIADMARGFGLGAPTGIEVIEEEAGNVPVPQSPVDATNLAIGQGETL
                     ATPLQVAVFAAALGNGGTLYRPQLVERIAPPDGPPTFEFKPEERGKLPISPENLKVIQ
                     DAMRGVVVST"
BASE COUNT         1606 a         2087 c         2256 g         1716 t
ORIGIN      
        1 cacaggagcg cagcttgggt gggatggccc taatcttggg gtcattggca ttgagcatgc
       61 cgctctcgcc gccttcgaca gccctgctgc tggtcatatt ggtcctgcaa gccattgcca
      121 tgggcaaggc atggttgcct tcgggttgga gagagcagcg ttggttctgg cccgccctgg
      181 ttggggcgat tttgctggtg gggattggcc tgtggttggc attgctgcaa tttgctccgg
      241 caggtgtcac cagccctata agcgtggtga gctggtggat caaaaagtcg gctgactgga
      301 acgcctatct aagcgaacgc gcctctggct gggtgcaaaa gatctttgac agcacgccgg
      361 agtggacgca catgccttta ctggtactgt atggcgtgat acagcccttc ctgccggcag
      421 cagtgattga cgtcaccgga gcaccggttt ggcggctgat cgccatctgg cgagcggcag
      481 gctggacgct gctgctacca ctgctcctat atgctactct gcgcgctctg cgcccaaaaa
      541 gcagcctgtc tgcctcgcag ggcttgtcgg cctccgggga acgggtgttg agcctggctg
      601 tttggttggt gatcctggtg gcttccttcc gcgggggcgg agacgcttgg gataacccgc
      661 gctaccgcct gacctttgcc ggactgcaga tggccctggt agcttgggcg tgggctgagc
      721 agcgtcgcct acccgatccc tggctgcggc gtgtgataat tggcttggga ttggtcctgg
      781 cttggtttgt gccctggtat ctgagacgct acctgcacat gcaatggcct gtggctgatt
      841 tttttatgac ccttggcctg gggctagcga gcgcagccct gtattggatt ggcgactggg
      901 gattggcgaa gtttaagcaa agaaaacaga gcgatgccga tcaccacgag tgatacatcc
      961 ttgcataggc gctcctccta tgatgattgg gcacattatg cttctaggag acaggactag
     1021 ccaaattttt atcgtttgtc aaagtttgcg tgtagttatg ctatactggt catgaacgtt
     1081 taacggtagg cgcaatttgg accatcccga ttctcattcc aatagaccta tacaataaca
     1141 gaaattgcag catggttgcg atgcttgtta tatcgaaaat cacatctggg gagcacctga
     1201 tgagacgaag tgaatatgaa taatcaacaa tcccaacctc gtgaacgtat tccagtcgcc
     1261 gtgttaggcg ctaccggagc cgtaggacag cgtttcgtcc agctattgga taaccatccc
     1321 tggttcaagg tcgtagtgct gacgggatct gagcgtgcca tcggcaagac ttatgcgcaa
     1381 gcctgtcatt ggatcctgtc cactccaatg cctgagtggg cgcgagagat ttcgatattg
     1441 ccagcggtcc ccgatgattc cgatgtcccc ctggtgtttt cggctttacc atcgtcgagc
     1501 gcttatgaag tcgaaccgta ctatgcgaga agaggcgtga tcgtctgttc aaacgcttcg
     1561 gcctttcggg ctgaacccga cgtgccactg gtgttgcctg aagttaatcc ggagcatatc
     1621 agcttgatct ctcaccagcg agaaaaaagg ggctggacgg gtgcgattgt taccaatgcc
     1681 aattgcacca tcaccggatt gactatcgtg ttgaaagccc tggcagatca atttgggatt
     1741 aataaagtct tcgtggtttc catgcaggct gtctcgggtg ctggatatcc gggtgtttca
     1801 tcaatggaca ttttgggaaa tgttgtccct cacatctacg atgaagagga caagatcgag
     1861 tcggaaccgc gtaaaatcat gggcacagcc aaggacgatc atgtggaact ggcagacttt
     1921 gaaatcacag cccatgccaa ccgcgtgcct gttttggatg ggcacatggt ctgcctgagc
     1981 attgaattgg cgaacaaggg gagcatagat gtggtggctg aagcgctaag aggctttcaa
     2041 gctccaccga ttagccgtga tctgccctcc gcgccgcggt ctgtcattga gctcaaagac
     2101 gagccggatc ggccccagcc tcgattggat atcatgactg gtcgtggtat gaccaccctg
     2161 gtggggcgtt tgagagaaga ccctgtcttg gattataaga tggtgattct ctcgcataac
     2221 accattcgcg gcgcggcggg agcttccata tataacgcag agttgcttgt caagcagggc
     2281 ttttttgagt aagcccgacg aggtatcgta atgtgccgaa gtattaaaac cctccgccgc
     2341 ccggagcagc cccccactga ggaggagttg cacgcggcgg ctctacaata tgtgcgtaag
     2401 gtcagcgggt accgcatgcc ttcccgtcgc aaccaagtag ccttcgagca ggctgtcgaa
     2461 gagatcgcta tggtcacgca aaaattgctg gacagcctgg tcaccactaa ctcccgttta
     2521 tcggggagta agtccatgaa aggagaatga gatgggcgaa agtgtctaca aagtgattga
     2581 attggtagga accagcagcg agtcgtggga gaaagccgca accgctgccg tggagatggc
     2641 tgccaagagc ctgcgggatc tgcgggtggc agagatttca gtgcttgata tgacccttga
     2701 gaatggaaaa gtgcgcttgt atcgtgccaa ggtgaaggtg tcgttcaagt atgagcgtga
     2761 agaggattaa aacctaaccc tttactccca ctcaatggtc gctggtggtt tgctggtgat
     2821 atcgtagacc acgcggttga cgccctgcac ttcattgaca atacggttcg cgactcgcgc
     2881 aagcaggtcg tagggtaagc gcgcccagtc cgcggtcata aaatcttcgg ttgttacagc
     2941 ccttagtgcc acagtctcct gataggtgcg ctggtcgccc atcactccca ccgattgcac
     3001 cggtaacagc acagcgaatg cctgggcggt gccttcgccg cgacgcccca tgcgcagtag
     3061 accagaggcg gccagttctt cggtcaagat ggcgtcagcc agcctcaggc gctccagtcg
     3121 ctcgcgggtc acctctccaa ggcagcgcac tgccagtcca gggccaggga agggctgacg
     3181 ccaaatcagg ctggatggca agcccagagc ctctcccacc atgcgcacct cgtctttaaa
     3241 cagatagcgt agcggctcca ctagctcaaa ccccatctct gcaggcaagc cgccaacatt
     3301 atggtgggtt ttaatgcgct gggctttggc tcgatctggc gcgctggatt cgactacatc
     3361 tggatagatc gtgccttgca ctaagaggcg cggcatgcct aactgccgcg cttgttgttc
     3421 aaagatgcgg atgaataatt ccccgattat gcggcgcttc tgctctggtt cggtcacgcc
     3481 gcgtagcgct gccatgaatt gatcgacggc atctacagtt accagctccg cgcctagggc
     3541 atcgtgaagg gcagcctgga cctgggcagt ttctccctgg cgcagaagac cagtatccac
     3601 aaacacggag acaagccgat cccccacagc gcggctcacc aaggttgtcg ccacgctcga
     3661 atccacccca ccgctgacag cagagagcac catctcttga cccacttgct ggcgaatacg
     3721 cgccatgctg ttcgtaatga tggattcagg cgtccattct gggcgagcgt ggcaggcatc
     3781 tagagcgaag cggcgtaaga tctccgctcc gcctggagtg tgatggacct ccgggtgaaa
     3841 ctgcacgccg aagtaaccgc gctgcaggtc acccatggcg gcaatcgggg agttatcgct
     3901 gcgtgccagg atcgagaaac ccgcaggagg ggtctcgatt cggtcgccgt gtgacatcca
     3961 caccgggtgg tccccatcgg ctaggagtgg attaggtttc aaggtttcta ccagggctgg
     4021 gccatactct cgctgggcgg caggggcgac gcgcccccct aacgcatggg ttagtacttg
     4081 catgccatag cagatgccaa gaacaggcag gtcgctttcc aaaatatacg gcaagagagt
     4141 tggcgcgtct ggctcgtaga ccgaggctgg tccaccagat aggataaatc ccttgggttg
     4201 cagggcaagc acctgttcgg gaggcgcatc ccaggggaat agctcgcaat agacctgcgc
     4261 ctcgcgcacc cgccgggcga tcaattgggc gtattgtgag ccgtaatcaa ggatggcaat
     4321 agtatccata aggaatcagg ggatagggga ttttttccct agacctgcta gctttatatc
     4381 actcgtcttc aaagaggatt gtatcccctt ccatggcgcg gatgcgcgcc agcgcctcga
     4441 tgggcacccc caggcctgct agcacctgac gcccgccctc gaaggtcttc tcgatcagcg
     4501 tgcccacccc cacgatgatc gccccggcag tctgtgccag tctagctagg ccaaggatag
     4561 tggcgccgct ggccaggaag tcatcgataa tcaggacgcg ctccccgcct gccagatact
     4621 cgggcgagac gatcagctcc accatgcgcc ctttggtatg ggaaggggag agtgtcaggt
     4681 acacctggtc gggcatggtg atgggtttgg tcttgcgggc atacaccacc ggcagaccca
     4741 ggtgcatggc ggtagacaag gctggggcga tgccggagat ttcagccgtg agcaccttgg
     4801 aggcgcccac gtcacgaaaa agccgcgcca gctcatgccc gcaagcctcc atcagctcgg
     4861 gatcgacctg gtggttgatg aatccatcca ccttgagaat gccgcccccc aggtttttac
     4921 cttcgcgaag aatccgttct ttcaaagcct ccatcgatcc tccttgcggt gaattgggtt
     4981 ttcctgatca gctcttagta atgcaagtta tttaatgctt actgtggatg tagtttcggt
     5041 agaggtaatt cacaaattgc cccaatgata atagtccaag gagattatga taaaatcatc
     5101 gggtgatgtt ggtgttggct tccaattcgc cgcgccgcag gcaattgatg gcattagggg
     5161 gatggacgtt tacgctcatc cccgcagcag ttgacgagcg acctcttcca ggagaaccgc
     5221 ccagggaata tgtgttgcga ctggccgagg ataaagcccg cacagtagct ggccgcgccc
     5281 cgcaggatgc gttagtgatc gccgcagata cagccgtggt ggatggtgcc gatatcctgg
     5341 gtaaaccgga cgatgcacag caagctgaaa ccatgctgcg acgcctgcgt gggcgctcac
     5401 accaagtata caccgccttg gcagtcttgc gtcttaccga tggcgttttg ctctccgatt
     5461 tgtgccaaac cgaggtcccc atgcgtcatt actcggatga agagatggca atctatatcg
     5521 ccagtggcga tccattcgat aaggccgggg catatgccat ccagcacgag gagtttaggc
     5581 cggtggaaca tctctcgggt tgttttgcta acgtaatggg tttgccctta tgccacctaa
     5641 cgcgcacttt gaagaaattg ggtgatccgc cagaacgcga tgtgccaact gcctgccagg
     5701 cagccctgca attcaattgc agtgtctacc cgcaggtgtt gacaggtgaa ttgtaaaagc
     5761 aatgaaattc caacactggt tgttcatttt actgatcgct ctacttgcca gttgtagcac
     5821 ctctccaagt gttactacta caccttcgtt ggttcccacg cgtaccctgg cacagcctgg
     5881 ggtgggtacc acgccggtcc ccgatcccgg ctcagttgca agtgcctttc tggatgcttg
     5941 gaaagcggat gactacgaga ccatgtatgc cttgatcact ccgcttagcc aggacgcaat
     6001 gagccaggag agtttcgtaa agcattacgc gggtattgcc gccgagatgg cgcttagtgg
     6061 gatcgattac gaggttctct cctccctggt ggaggatccc agtagcgctc aggtgggcta
     6121 tcgggtgatt tttcacagct cgctggtggg ggatatccaa cgtgaaacgc tcatgaactt
     6181 gagcctggac caaggtgagt ggcgggtgaa atgggatgat tcgctggtga tgcccgaatt
     6241 ggctggcgat aacttcatgg gtatggagcg ttatatcccc gcgcgcgcta atatctacga
     6301 tcgcaatggg catgctctcg tggctcaaac agatgtaacc gccattggtc tggtacccaa
     6361 ccagatcgac cccgaccagg aggagaagct ctacagctgg ctggtgcgcc tgacgggcct
     6421 cacaggtgac gagatcgtct ctcgctacgc gagcttcccc cccggcgtgg attggtactt
     6481 gcctctaggt gaagtggctg cggagaccat cgccagtaat attgacatcc tttccggttt
     6541 cagcgggctg gttctggggc cataccgggc gcgttattac tttgatggtg gcatcgcacc
     6601 gcatgtggtg ggctatgtca ccaccatcca agcaggtcag gaagaggagt atctgcgcaa
     6661 aggataccag cgcgatgaac gcgtgggcca ggccggtctg gagaaatggg gagaagatta
     6721 cctttcgggg gtgcgcggcg gcgccctgta tgtttttaac ccccaggggc agatcatcac
     6781 ccggctggct gagcgggagg cacagccctc ccaggcgatc tataccacgc tggaccgtga
     6841 tttccagcaa gaggtcgagc gagctatctc aggctaccgc ggtgccgttg tggtgctgga
     6901 gcgcgacacc gggcgtgtgc tggccatagc ttcttcgccc agctttgacc ctaacgcttt
     6961 tgagccaaga aaccgcaact cgtctgccat gctggaggat atttttagcg atccctccac
     7021 ccccaccttc aaccgcgccg ctcaggggca atatcccccc ggttcggcgt tcaagccggt
     7081 gaccatggca gcagcactgg agagcggcct gtacactccg gagaccactt accagtgtgg
     7141 ctatttcttc aatgagttgg ctggcctcac gctcagggac tggacatacg aacactatct
     7201 cgaggacggc aagaccatcc ccagtgggtt gctgaccctg ccagaaggtt tgatgcgctc
     7261 ttgtaatccc ttcttctggc acatgggtct ggatctatat aaccaggata agaccaccgc
     7321 catagcggat atggcgcgtg ggtttggtct gggagccccc accggcattg aggtgatcga
     7381 agaggaggct ggcaatgtgc cggtcccgca aagcccggtg gatgccacca acctggcgat
     7441 tggtcagggg gagacgctgg ctacaccact ccaggtggcg gtttttgcgg ctgccctggg
     7501 taatggaggt actctctatc gtccacagct cgttgagcgc attgcgccac ccgatggacc
     7561 tcccaccttc gagttcaagc ccgaggagcg cggcaagctg cccatcagcc cggagaacct
     7621 aaaggtgatc caggatgcca tgcgcggggt ggtggtgagc acaaa
//
LOCUS       DBNHOH010000742         2486 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1456593, whole genome shotgun sequence.
ACCESSION   DBNHOH010000742 DBNHOH010000000
VERSION     DBNHOH010000742.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1456593"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="AC16V7_12950"
     CDS             <1..479
                     /locus_tag="AC16V7_12950"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6258074.1"
                     /translation="PYVILGVSLLIMFHQVGISLSAFTAGLAHVVVSVPYALLVIASR
                     LVGFPQNLEEAAMDLGCTYWGALLRVTLPISFPALVAAFLICFTISFDEFAIASFLVG
                     TQPTLPVYLYSQLRFPTRLPLVVSMASILMTGSMILILLSEWLRRLGQTSGGSAKS"
     gene            476..1585
                     /locus_tag="AC16V7_12955"
     CDS             476..1585
                     /locus_tag="AC16V7_12955"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6258075.1"
                     /translation="MNETIVELRNVTKIFTSRRGATLEEVQAVKDVSLNIEQGEFFTL
                     LGPSGCGKTTTLRLIAGFEIPTSGEVLIQGNRMSRTPPYQRPVNTVFQNYALFPHLSV
                     LKNVAFGLAVKRMARAQREVLAQEALDLVRLAAMGDRKPSQLSGGQQQRVALARALVN
                     KPAVLLLDEPLGALDLKLRKAMQLELKQLQEQVGITFIYVTHDQEEALTMSDRIAVMK
                     DGIVQQLGDPVTLYERPANRFVADFIGESNLIKALVESISDRRVDLRIGQEQMTASVG
                     DAPPAPGQEIVVAIRPEKLRITAEKEILAQAMSGTVSQVIYIGTDTRYVVDLASGESI
                     TIRVQNSFGRDLEEFKRGDRVRISWAAEDARTLLV"
     gene            1626..>2486
                     /locus_tag="AC16V7_12960"
     CDS             1626..>2486
                     /locus_tag="AC16V7_12960"
                     /inference="COORDINATES: protein motif:HMM:NF018012.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR6258076.1"
                     /translation="MTLQAIDHQLPKPYRYSRLTDEQFQLVHSASLEILERIGVRVDL
                     GEALDLLKHAGARVEDNLVRLPPRLVEQALQTAPKQVTLFNRHGEPVMPLDGDRCFFG
                     PGSDCLNIIDHRTGERRKPVMQDVVEGMILCDALLNIDFVMSMFLPANVDQTIADTYQ
                     AEVMLSHTTKPIILVSYGASGLRNAVEMAEVVVGGPEALRAHPLLTCYINVVSGAVHN
                     EECLQKLLYRAEKGLPSIYIPGSNAGVTSPISMPGAVALDMAGGLLGLVLSQLKREGT
                     PYILSAMDPAA"
BASE COUNT          518 a          726 c          699 g          543 t
ORIGIN      
        1 tcccatacgt tattctggga gtttctctat tgatcatgtt ccaccaggtg ggcatatcgc
       61 tctcggcctt caccgccggg ctggcacatg tggtcgtcag cgtaccatat gccctgttgg
      121 tcattgcttc ccgcctggtg ggcttcccgc aaaacctaga ggaagccgcc atggacctgg
      181 ggtgcaccta ctggggagca ttattgcggg ttacactgcc catcagcttc ccagctctgg
      241 tggctgcctt cctgatctgc ttcaccatct cctttgatga atttgccatc gcttcgttct
      301 tggtcggcac gcaaccaact ctgccagttt acctgtattc gcagctgcgc tttccaaccc
      361 gcctgcccct ggtcgtctcg atggcatcta tcctgatgac gggctccatg atcctgattc
      421 tgctgtccga gtggctacgc cgtctcggcc aaacatctgg tggaagtgct aaatcatgaa
      481 cgaaacaatt gttgagctac gcaatgtgac gaagatattc acctcgaggc gcggcgccac
      541 cctggaagag gtgcaggcag tgaaggatgt cagcctgaac attgagcagg gcgagttctt
      601 taccttgctt ggtcccagcg gttgtggcaa aaccaccaca ttgcggctga ttgccgggtt
      661 cgagatcccg acatccgggg aggttctcat tcagggtaat cgcatgagcc gcaccccacc
      721 ctaccaacgc cctgtcaaca cagtctttca gaactacgcc ttgttccctc acctgagcgt
      781 gctgaaaaat gtggcttttg gcctggcagt caagcgcatg gcccgcgccc agcgcgaggt
      841 cctcgcccag gaggcgctcg acctggtccg gctagccgcg atgggcgacc gcaagccatc
      901 ccagctctcc ggaggtcagc agcaacgcgt ggcgctggcg cgcgccctgg tcaacaaacc
      961 tgccgtgttg ctgctggacg agccgctggg ggcattggac ctcaagttgc gcaaggccat
     1021 gcagctcgag ctcaagcagc tccaggaaca ggtaggcatc acgttcatct atgtcaccca
     1081 cgatcaggaa gaagcgctga ccatgtctga ccgcatcgcc gtgatgaagg acggcattgt
     1141 ccagcagctg ggagatccgg tcaccctgta cgagcgaccc gccaaccgct ttgtagccga
     1201 cttcatcggg gagagcaacc tgatcaaggc cctcgtcgag tcaatatctg atcggcgcgt
     1261 cgacctgcgc atagggcaag agcaaatgac agccagcgta ggggatgcac cccccgcacc
     1321 gggtcaggaa atagttgtgg cgatccgacc ggagaagctg cggatcaccg cagaaaaaga
     1381 aatactcgct caagccatga gtggcacagt gtcgcaagtg atctacattg gcaccgatac
     1441 tcgctacgtt gtcgatcttg cctccggtga atcgatcacc attcgagtgc aaaacagctt
     1501 tggcagagac ctggaagagt tcaagagagg cgaccgcgtg cgcatctcct gggctgcgga
     1561 ggatgcgcgg acattgttgg tttgatttat ttctacgaag ggaaaaaagg ataaccattt
     1621 tcaagatgac actgcaagcc atcgatcacc agctgcccaa gccttatcgc tactcacgcc
     1681 tcaccgacga gcagttccag cttgttcact ctgcaagcct ggagattcta gagcggattg
     1741 gcgtgcgcgt ggatttggga gaggccttgg atttgctcaa gcatgccgga gcgcgtgtgg
     1801 aagacaacct ggtgcgtctc cctccgcgcc tggtcgagca agccctgcag acagccccca
     1861 aacaagtgac cctgttcaac cggcatggcg agccggtgat gcccctggac ggtgatcgct
     1921 gtttcttcgg ccctggctcg gactgcttaa acatcatcga tcaccgtacg ggcgagcggc
     1981 gcaagccggt gatgcaagat gttgtcgaag gtatgatcct gtgcgacgcc ttactcaata
     2041 tagattttgt catgtcgatg ttcctgccag ccaatgtaga tcagaccatc gccgacacct
     2101 accaggcgga ggtcatgctg tcacacacca ccaagccgat catcctggtc tcttacgggg
     2161 caagtggcct gaggaacgcc gtagaaatgg ccgaggtggt ggtgggtggt cccgaggcgt
     2221 tacgcgctca tcctctcttg acttgctata taaatgtagt ttccggcgcg gtgcacaacg
     2281 aggaatgttt gcagaaactg ctctatcgag cagaaaaagg attgccatcc atatatattc
     2341 ccggctcaaa cgccggtgtg accagcccga tcagcatgcc cggtgcggtc gccttggaca
     2401 tggctggtgg cttattggga ttggtgctct cgcagctcaa gcgtgagggc acgccttaca
     2461 ttttatccgc tatggacccg gctgcc
//
LOCUS       DBNHOH010000743         1680 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_45555, whole genome shotgun sequence.
ACCESSION   DBNHOH010000743 DBNHOH010000000
VERSION     DBNHOH010000743.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_45555"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="AC16V7_12965"
     CDS             <1..439
                     /locus_tag="AC16V7_12965"
                     /inference="COORDINATES: protein motif:HMM:NF024247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR6258077.1"
                     /translation="FFLERHPKLAPIETANEGVFLAGACQSPKDIPDSVAQAGAAAAA
                     ALSLIDQGTIALDPSIAEVNHLHCAGCGQCSNACPYGAIEVKDGAAVINGFLCKGCGT
                     CAGSCPNKALTLIHFDDRQIISEMIGALKAPVATSVLSVQEAG"
     gene            436..>1680
                     /locus_tag="AC16V7_12970"
     CDS             436..>1680
                     /locus_tag="AC16V7_12970"
                     /inference="COORDINATES: protein motif:HMM:NF014692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HIR6258078.1"
                     /translation="MMTFEPKIITFLCTWCSYTGADTAGIARLKSPSNIRAIRVPCSG
                     RVAPEIIMKTFDQGADGVLVLGCHIGECHYDSGNHRTAKRLPILQSLMAFAGLEPERL
                     HLDWVSASEGERFSRIVSEFTESVRLLGPARWKSENRQPRFDQTQLAIDRSYTFDQSS
                     YSEEVERSISFTNNLQAKARELLSCGKVACVIGYEKGTHNRTRPVFIYTEDAVERLVW
                     NKDCTHNLTVYLRRAIYPPLGKQNGEPGKSVAIVAKPCDSRAINVHLAGNHFQRDQVH
                     VIGVACDGILDKQQQYRMDHVLQERCLHCTERLPVIYDTLIGEPPANQVEPTTSARLY
                     HLETLSPTERMDFWLSQFDRCIRCYACRQVCPMCDCPTCLFERDDSLWVGPGIRLDEK
                     RIYHLGRAYHLVGKCVDCHECER"
BASE COUNT          428 a          411 c          428 g          413 t
ORIGIN      
        1 gtttttcctc gaacgccacc ctaagctcgc cccaatcgaa acagcaaacg aaggagtatt
       61 cctagcaggc gcttgtcaaa gtccaaagga catccccgat agtgttgctc aggcaggcgc
      121 agctgcggct gcagcgctct ctctaataga tcaaggcacc attgccttag acccttcaat
      181 tgcagaagtt aaccaccttc actgcgctgg ctgtggacag tgttcgaatg cctgccctta
      241 tggagcgatc gaagtaaaag atggggctgc tgtgattaat ggcttcctat gtaaaggttg
      301 tggcacctgt gctgggagtt gcccgaataa agctttgact ctcattcatt ttgatgatcg
      361 ccagatcatt agcgagatga ttggagcact aaaagcacct gttgcaacct ctgtattgag
      421 cgttcaggag gcgggatgat gacatttgag cctaaaatca tcacatttct atgtacatgg
      481 tgcagctaca caggagcgga tacagcaggc atcgcacgct tgaaatctcc gtccaatata
      541 cgggctatac gcgttccatg ttctggtcgg gtggctccag agatcattat gaaaacattc
      601 gatcagggtg cagatggggt tctggtgttg ggatgccata tcggtgagtg ccattatgat
      661 agcggtaatc accgaacggc taagaggtta cctatactac aatcactcat ggcttttgct
      721 ggactagaac ctgaacgttt gcacctggat tgggtatccg cttccgaagg tgagcgattt
      781 agccgtatcg tcagtgaatt caccgaaagc gttcgattgc tcgggccagc cagatggaaa
      841 tcggagaata gacaacctag atttgatcaa actcaattgg caattgatcg atcatacacc
      901 tttgaccaaa gctcatattc agaagaagta gaaagatcaa tatcctttac taataaccta
      961 caagccaagg caagagagct gctctcttgc gggaaggttg cctgcgttat tggttatgaa
     1021 aaaggcactc acaaccgaac ccgtccagtc tttatataca ccgaagacgc ggtcgagcgt
     1081 ctggtctgga ataaggactg cacacacaac ctgacagtct acctgcgcag ggcgatttac
     1141 ccaccgcttg gtaaacaaaa tggtgagccg ggaaaatcgg ttgccatcgt agctaaacca
     1201 tgcgacagtc gggcgatcaa tgtacatcta gcgggaaatc acttccagcg cgaccaggtg
     1261 catgtgatcg gagtggcttg cgacggtatt cttgataaac agcaacagta tcgaatggac
     1321 cacgtccttc aggaacgctg cttgcattgt actgagcgtc tgccggtcat ctatgatacg
     1381 ctcatcggtg aaccaccagc gaaccaggtg gagcctacta cctccgcgcg cttatatcat
     1441 ttggaaactc tatcaccgac agagcgtatg gatttttggc tttcccagtt cgatcgctgc
     1501 atccgctgct atgcctgcag acaggtctgt ccgatgtgcg attgcccaac ctgccttttc
     1561 gaacgggacg attcgctttg ggtgggccca ggcatcaggc tagatgaaaa gcgaatttac
     1621 cacctgggtc gggcttacca tcttgtgggt aaatgcgttg attgtcatga gtgcgaacgc
//
LOCUS       DBNHOH010000744        42822 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_172972, whole genome shotgun sequence.
ACCESSION   DBNHOH010000744 DBNHOH010000000
VERSION     DBNHOH010000744.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 42822)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 42822)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42822
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_172972"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="AC16V7_12975"
     CDS             <1..195
                     /locus_tag="AC16V7_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258079.1"
                     /translation="ASLEVSERDLHDGEIRAEWPEDIAECILLAVDYHLEGCEKTTKR
                     YTSMRSLSNRLIGALNGVEQ"
     gene            192..512
                     /locus_tag="AC16V7_12980"
     CDS             192..512
                     /locus_tag="AC16V7_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258080.1"
                     /translation="MSAIPEVFTKSQINEEVVSAAARLLELRAELLRTAARMRALGER
                     LVMAPRWNYSGDIDAVAVNKLFAGEDEAQALGHLSVLLDVLADHTKAAVAEISSDAKF
                     RGES"
     gene            509..709
                     /locus_tag="AC16V7_12985"
     CDS             509..709
                     /locus_tag="AC16V7_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258081.1"
                     /translation="MKLEIRTFQIEHKGVQQLWLRMESHRTVLEFATSVEGLQRLHTQ
                     IGISLESARAGFMVQATDVGEV"
     gene            706..1122
                     /locus_tag="AC16V7_12990"
     CDS             706..1122
                     /locus_tag="AC16V7_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258082.1"
                     /translation="MSDDPVEYKVRSPEPLAQVEPEVDYYRRKLRQLRAWLEELDQAG
                     PEDLGRALMSSHSKIRQGVIDLLDRPRSNGPAIDRLERELGELRGKLIDFDIMRKELA
                     AAKEAAQLWQRRAEGGPVKTAVVSAAGRLDRWLGRK"
     gene            1119..1541
                     /locus_tag="AC16V7_12995"
     CDS             1119..1541
                     /locus_tag="AC16V7_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258083.1"
                     /translation="MRQTSIDAWEDIKASGVLGLEQLRAYECLFRHGPLTGREIDDRL
                     GVDMHKRLSELERLGVVKCNGKRECRVTGRQVHEWDVTPYRATRIAKRAARTGTKNKS
                     TTKALLELLKEACKALDESGDLFARSKAHEIRRKVGEL"
     gene            1906..2133
                     /locus_tag="AC16V7_13000"
     CDS             1906..2133
                     /locus_tag="AC16V7_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258084.1"
                     /translation="MRDGIDDGLEALLRMQEIWPRPPGGSAAWSGCQLVISIFSSLPA
                     LADELGVTMDTMTVDDIVVAMKSSAERGLKP"
     gene            complement(2144..2386)
                     /locus_tag="AC16V7_13005"
     CDS             complement(2144..2386)
                     /locus_tag="AC16V7_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258085.1"
                     /translation="MAKEFEARWLGHRTKALACRGLKKSYKSFSSGLQDRAGAERMLG
                     MAHDLKCSWQRPVIKKLSKAQRSRIVDPISFGPPIR"
     gene            complement(2391..2639)
                     /locus_tag="AC16V7_13010"
     CDS             complement(2391..2639)
                     /locus_tag="AC16V7_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258086.1"
                     /translation="MAKKKLFLTPSYIPDPKNASKCEAVMVRSPYAMYPIARVCKRDN
                     GTWGYSSVGPSPHTLHGNKKTKMLAVKAAVKAYKSWIG"
     gene            2796..3230
                     /locus_tag="AC16V7_13015"
     CDS             2796..3230
                     /locus_tag="AC16V7_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258087.1"
                     /translation="MKARYLFLGTALLSGCSLLFEPTGGTDAASDSFGGDASLPADGG
                     SIDIDAMAVAADAALCDPFTDPDCYVDNYPLGGPYPRPGGAGVDGSDCTLSSECAASM
                     FCTGVAGTEGYCLPLCDAEHQCGGDRACLSTDWQSYGFCELL"
     gene            3246..3845
                     /locus_tag="AC16V7_13020"
     CDS             3246..3845
                     /locus_tag="AC16V7_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258088.1"
                     /translation="MAATKPEASKPMSQLDLFGQPVADPEESPKPPAKPRPAPKPKPS
                     PPECPVRVQGPADEPCVRCRERFEHHFAGALLCAACADALVDIQLEDRCIVIGCGCVT
                     MGDEIDGHPICGLHNTDVTRAIMATGAWPAWYGYAGKWIVVATVRRLDDMGHTWAGIA
                     ADKHCGACGLPYATWTKDRFTAACTDYAQPDTTKGLFRP"
     gene            3980..4465
                     /locus_tag="AC16V7_13025"
     CDS             3980..4465
                     /locus_tag="AC16V7_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258089.1"
                     /translation="MIAPGNNALEYCPYGQVGDQLWVRETFCQVDDTDHGGELWVDYR
                     ATPKYSEEHPAGWENAPCDHEALKWKPSIFMPRALSRIQLDVLSVRVERLRDISVQDI
                     VDEGINAEVRVLDHDVAEGTVAQVLKHRFMELWDGINAKRGYGWDTNPWVWAVEFGVI
                     E"
     gene            4462..4632
                     /locus_tag="AC16V7_13030"
     CDS             4462..4632
                     /locus_tag="AC16V7_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258090.1"
                     /translation="MSTKAEKKRRQFAERERRRKIEEELSKNWICDHCKIMVQHWSPI
                     CLCGRLRPEVKL"
     gene            4629..5543
                     /locus_tag="AC16V7_13035"
     CDS             4629..5543
                     /locus_tag="AC16V7_13035"
                     /inference="COORDINATES: protein motif:HMM:NF052956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcrVA2 family anti-CRISPR protein"
                     /protein_id="HIR6258091.1"
                     /translation="MKRIGTIPDLYAAAHFIAKSSTSQQYVDTFRDLETCSDERARDL
                     IRLLGVTSREEAEEYLNMMMITYVASTSWISQGCQLFSLDERTMDLAVATFPVSLKEF
                     HMGNDPEESKDEWRLPYETFAIEFPFLVAVDMHNSEVKARRLIVEPFRNVHDEFAWNV
                     LWTDVTNDMSVSVVTEGTVLYSLITNLCFFLNETGTITRTRPSYGFTGRAKSAARAVK
                     AWSASFHQVTNLPLGIAKEFLASGKPPGEQFRMGKRIMVRGHWRLQPCGPGRQFRKHK
                     WIAPYMKGDEDDPLWTRTYKVTPGKLEL"
     gene            5540..5926
                     /locus_tag="AC16V7_13040"
     CDS             5540..5926
                     /locus_tag="AC16V7_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258092.1"
                     /translation="MSIGGELFVCVRCLHLLRPGEPVDHFEPDWFDYGGSASRCTSHG
                     ACDERVKERRAQHREEIERRVRHLKEGECLLVIQDQFGAKYDSYGNYYVTVAEEDAQD
                     EWDKRQEEVKENGEEGCLVLSETRRQ"
     gene            6110..6316
                     /locus_tag="AC16V7_13045"
     CDS             6110..6316
                     /locus_tag="AC16V7_13045"
                     /inference="COORDINATES: protein motif:HMM:NF046381.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrdR family transcriptional regulator"
                     /protein_id="HIR6258093.1"
                     /translation="MKCLCGGDTSVSDSRTVPNNPHSTRRRRECLACGKRFTTYEVMD
                     SMATITVRLRSGRIVIEHSKKDEK"
     gene            6511..6900
                     /locus_tag="AC16V7_13050"
     CDS             6511..6900
                     /locus_tag="AC16V7_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258094.1"
                     /translation="MDSHRALSSATIDIFRAATINHKPWVDEAEGINMELGDCPSCGS
                     SLCWSFDEVLASPTMEMRVEELEAWLSHHKLGLSVSFNERCYSAELRTKIGNESIVAM
                     EAKAAEFGVAVIAACQNYEREYAQRID"
     gene            6851..7756
                     /locus_tag="AC16V7_13055"
     CDS             6851..7756
                     /locus_tag="AC16V7_13055"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR6258095.1"
                     /translation="MRPVRTTKGNMPSALTEVIERSTRLGEKSKEKYMLCVERFVDFA
                     GEDPVGWSGAAVERWRDHLRADKGAGTVNGHLYALRYASRRLEGLGIGVDFARAAESL
                     PDPKTRKRKSMSLEEVKALIETCDRQRPMDLRDRAIIVIALRTGLRASNLVGLSWGGI
                     KGRVADCVIKGNKHHQVILDDRCLGELQRWGDCLESSGRSLRGPIMRRVSELITGDYH
                     IGNPIKRRAWVNEMLRSRCAAAGIRNVHAHLFRHTFVSWALEAGVAPHRVMRQTGHSN
                     MATLSQYVTDLEAETNPIGDHLPEF"
     gene            7756..8055
                     /locus_tag="AC16V7_13060"
     CDS             7756..8055
                     /locus_tag="AC16V7_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258096.1"
                     /translation="MRHKDEPEETDLDETICALHVALREVAFHTMAAQGARDGLEDCF
                     LHMGDPEISESADVALWANRVSMAYQAVADVAYSVSIEAGIVARIMEPTDKETEN"
     gene            8061..8372
                     /locus_tag="AC16V7_13065"
     CDS             8061..8372
                     /locus_tag="AC16V7_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258097.1"
                     /translation="MKRRVEPEAREVILQVRPVADAEHPWWPKLEGWYRWASIDPYDP
                     PGWSMGLDEDYAIDSIELWKQTKTTLDQHIEHMKSEWLEECGPDEEGDGFLWEFRVVN
                     R"
     gene            complement(9024..9671)
                     /locus_tag="AC16V7_13070"
     CDS             complement(9024..9671)
                     /locus_tag="AC16V7_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258098.1"
                     /translation="MLSISMDGFGAIDPMLGFMPNLREGSDYRMGTTRSGPVALALTA
                     PVLAMFKSIQVSINQVGQLMGMPLSLTVDGKMGNATGSALKLVVNRLDPDLQRNLSPE
                     AKAGQAAGVAKDARNVLGTLQEAAVRRDKAPALPVAAVAPLPPVAPSPAPTRLQLPDI
                     KSNIPLVRPAITPVSQRFVSTADERKKMSTGVFVGVLAAFLLAGGAAAVLVARRD"
     gene            complement(9683..9868)
                     /locus_tag="AC16V7_13075"
     CDS             complement(9683..9868)
                     /locus_tag="AC16V7_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258099.1"
                     /translation="MLPIVYSYLNEDKRGRLEYIHSMNLKALEQVMQVEENEKLWLEA
                     ANAKRAILIQYELERKK"
     gene            complement(10179..10391)
                     /locus_tag="AC16V7_13080"
     CDS             complement(10179..10391)
                     /locus_tag="AC16V7_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258100.1"
                     /translation="MMWTRNCGPDAVYALVRANRNIAHARTHLASIGFGSSSPRTRRL
                     WAFVNKRERQIDLAGNRVAKCMLKGK"
     gene            complement(10484..10807)
                     /locus_tag="AC16V7_13085"
     CDS             complement(10484..10807)
                     /locus_tag="AC16V7_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258101.1"
                     /translation="MSLSAVTVGPGYGINGSLGAYDDVTLVGGDMGFVEIEPGYVVNG
                     AYPDYGPMYGAGMGAYDDVTLVGDFGLLPEKGIWSRNMLLGTAAGLLATAATAYLLGK
                     AMAFK"
     gene            complement(11191..11670)
                     /locus_tag="AC16V7_13090"
     CDS             complement(11191..11670)
                     /locus_tag="AC16V7_13090"
                     /inference="COORDINATES: protein motif:HMM:NF013704.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HIR6258102.1"
                     /translation="MCFHTSHDLMPHWEESLLAAGRYVFPPVPCVMSGMSVRLQGDGP
                     SSWTCWLVVARPRRKEFMSWGTLPGAYVGPPPSRASSRGEKSIVMGQKPIWLMSALVR
                     DYSRPGDLVCDPFAGGGTTAVACESLGRRFIGSEVEPETHARASSRISGGVQIDLFG"
     gene            11951..12163
                     /locus_tag="AC16V7_13095"
     CDS             11951..12163
                     /locus_tag="AC16V7_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258103.1"
                     /translation="MGKSKPKSGAVLLATWLEKNKVTLAAFAADAGCSKQAVGAIRSG
                     AFKPGLALAVRIERATKGKVPTISWA"
     gene            12173..12409
                     /locus_tag="AC16V7_13100"
     CDS             12173..12409
                     /locus_tag="AC16V7_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258104.1"
                     /translation="MSPEDKDLICRMAADIAGPLLAQAAAHPRGGGLDVELLMNSEAC
                     ASMHRTVAKHALAASWQIINEIESKLEEKLSPPE"
     gene            12531..13979
                     /locus_tag="AC16V7_13105"
     CDS             12531..13979
                     /locus_tag="AC16V7_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258105.1"
                     /translation="MLSATQIGNVPAEVKSAQRELLALAEEVRRSWPPNQTEYRSDDV
                     RKVVTSLNRSVAKLVESVSLAVQDPLIAESEGLVAQANRGNAELQKLQRALAIPAEQS
                     RQAATVDLPAFKGAIPAAMIRVAQVWGDLNTLHRVSLGWIGGTQADIMVQGYRDVLAA
                     VQGMPGAKKIGFVGRHRKKIAIGVVAVAASGAGTWWILRRRRTMMLQAPEMDAPEEDL
                     EIPVGIHFLPEGNSKLAKASELVNHIARPDELGAPSFALRDLPRGMREEMFNLHATSV
                     NPDATERAFAKKRVPVRDVSIAKIDVSSYKDEDYFGKQDLASLPPIVIADGKLIDGGH
                     RVAAAQRKGIKTLRAIDMTGLLDPDSTGSIAELRRAGKLGGEQSALPPGFEPFDVDYD
                     APRKTKPECGEHLSAMRRGDVVQVGGSRWEVTHARGTDVLVIKHGTKGRKLYKLVTVS
                     LDPCEVEVQEVHPGSGDIMHNVAPSARGALGD"
     gene            complement(13976..14746)
                     /gene="bet"
                     /locus_tag="AC16V7_13110"
     CDS             complement(13976..14746)
                     /gene="bet"
                     /locus_tag="AC16V7_13110"
                     /inference="COORDINATES: protein motif:HMM:NF015776.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HIR6258106.1"
                     /translation="MTSATQPATVSPIQGSFTAEQIDLIRRTICPDATDDELALFVQR
                     CKSTGLDPFTGQIYLVKRKGSPATMQTGIDGFRLVAQRTGERDGMDAPQWCGKDGNFL
                     PIWTGSEPPVACSITVYRKGHSRGYPALAYWDFYAQTKAGGELNYMWSKGGPHMLAKC
                     AEALALREAFPNELSGLYTTDEMHQASEVTTMLDAPQITAEIAAEATRVIDALRATKT
                     KEEMAELCPRIKLLPEAGKVMVRATWKANVARLEAQEG"
     gene            14959..15162
                     /locus_tag="AC16V7_13115"
     CDS             14959..15162
                     /locus_tag="AC16V7_13115"
                     /inference="COORDINATES: protein motif:HMM:NF024949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6258107.1"
                     /translation="MKIERATSGLSQSELATLLGVAKIDVQRFESGAKAIPLDLLPRL
                     CECLGVTASRILDDYRGLITRAA"
     gene            15159..16139
                     /locus_tag="AC16V7_13120"
     CDS             15159..16139
                     /locus_tag="AC16V7_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258108.1"
                     /translation="MTINPKMWHDRHFVGLSVDAKLLWCFLLTGNNDGVPGLIRGGPG
                     AYSDAMRWDRPTTASALDELIKATFIEYDQHAQLLRVPGAPKYSPCTNANVLKGWLRR
                     WKDVPESPLKYHHIESLRQAAEDRPWFEEAWSVTFAPIKVPDHTKQVPLFLISPAKGS
                     IASESEVLNPLANPLANASERVCETNNTYTDTTTDTYTDTDTSTPSRERPRVRDPMER
                     HVFASELWRIQEELRSELAPNVVTRPPTGHDLIPIMQALEIYSREELENSLRVNAATA
                     ANKPEKLEYFNGYSNWHPNSLRRTVGQVVPTSSGRHSVRGRKKFKGGNVL"
     gene            16136..16816
                     /locus_tag="AC16V7_13125"
     CDS             16136..16816
                     /locus_tag="AC16V7_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase family protein"
                     /protein_id="HIR6258109.1"
                     /translation="MKKNQSSSVGESLKKSIAKAWAAFDDPENQKRIAELNAAASDKD
                     VRTQRHLRIKKLRRWGVPKIASEIVVDGPLDKTSAVLAVGGDEVALLRVLAGNPGCGK
                     TIAACTRIEDRETGLFVRAAELVELGSHYSDRNLLSSYRRCGVLVIDDLGIEYRSDQM
                     ICRLDELLDSRAGAGMPTIITTNFTADEFRARYEERLWSRIHQFGSYVELDDPDLRVT
                     PIGQGRRP"
     gene            17135..17317
                     /locus_tag="AC16V7_13130"
     CDS             17135..17317
                     /locus_tag="AC16V7_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258110.1"
                     /translation="MDDSEKISLNAFSTSREACARRSDIQRLAIKYYSADMTVSWIDC
                     VAMARIELDAAAVVEN"
     gene            17317..17658
                     /locus_tag="AC16V7_13135"
     CDS             17317..17658
                     /locus_tag="AC16V7_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258111.1"
                     /translation="MPKTQPWTPEPPVWEWNGISERFRARAKERYAQRSSAIESDGQA
                     RGPAELRAYCELVEVMHEKGVFNTEHIRSHDVIRQLHRAENFKRHDAIGRRILAGTWP
                     KREKGEPPRTI"
     gene            complement(17692..18411)
                     /locus_tag="AC16V7_13140"
     CDS             complement(17692..18411)
                     /locus_tag="AC16V7_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258112.1"
                     /translation="MSNTEIIGPSGNRAEVSDSKRILSSAAFYDMLAKVSADDAEAYI
                     MYSGQMVIPSHANVTPLNDAPGPGQTSRGYAVHWFRNSHATKNLIIHSASWGSNGGSD
                     PSKSQIFECKPIFASHLLPPTDNISTLFETIPKRQNIANQSLPEIEAYGWNSDTAAAV
                     PNGLDMPIGSYNPARKIMNFLVQRGYQDTSFESAFILPPGSPAILHIFDAPSLLAGET
                     LDCHITIKAWHAPVCNPWLGL"
     gene            complement(18482..18634)
                     /locus_tag="AC16V7_13145"
     CDS             complement(18482..18634)
                     /locus_tag="AC16V7_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258113.1"
                     /translation="MSYAPKRNVDDAALVSDGDRKQIATTDNQTQNLLFEILQELRQI
                     KEKLSE"
     gene            complement(18647..19276)
                     /locus_tag="AC16V7_13150"
     CDS             complement(18647..19276)
                     /locus_tag="AC16V7_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258114.1"
                     /translation="MRNVLKAIVSFVAVAPGATVELPHGLNISGTSVIPDEIKRRTEG
                     LFQITADKVNVRVKNLGNSDADVDVLCERWHGVPRELGIGNDGYGWKTNLPIQPWQSQ
                     GDANDDAGSCCPFIVNVDYTNNPYNAKNGDVILVDSSIAPIVINAPSASAPGSINDTF
                     TVKNFKGGCSTHGITVVPDGTDVIESPTIDADKVSKTWISDGAGNWVII"
     gene            complement(19280..20725)
                     /locus_tag="AC16V7_13155"
     CDS             complement(19280..20725)
                     /locus_tag="AC16V7_13155"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR6258115.1"
                     /translation="MPIFPVPILSGIIPKVSDHFGFSEQRGRLHAGADIMYPRQQAGD
                     LRLPEFSRRFFMPSDVPALAFNAGVVTRAGQIGTGGRVEIDHGNGFSTKYFHLSKLLV
                     KAGDAVKAGDSVGIIGHNPDGFRLNHLHFEILQNGRQIDPEPLLVKAQQVALEPGQVT
                     GGSFSQSVRKGAVIGIALIAGAGVLALLARRGRKGAGLGFSGLDIQVTNKPEKFGSPA
                     GFEVCATDSEGKKQGCVTARRAAWMPYDERNGVKHGDPLPLEVVSSWVHGKGRGLGTE
                     LYHAAAVEACRRGVTLQSDMDRSDAAEGFWKKQIRKGRAVRRVAYLTEEKVTYYRLSC
                     PAPDTLGAAKHSLYSMPDRWWVTKVPKDRLCYKTKTEALRKFMDWNTGVVEDYGGPTA
                     KGSRGEFDALTKFEVSPTNIAEALWVSLPAPPRGRQYCLEDIDVEALNDTSPGKDHPV
                     GFQLPDFVLESRLVEDEYEYYRHRGTDGLRK"
     gene            complement(20725..21354)
                     /locus_tag="AC16V7_13160"
     CDS             complement(20725..21354)
                     /locus_tag="AC16V7_13160"
                     /inference="COORDINATES: protein motif:HMM:NF015935.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HIR6258116.1"
                     /translation="MAKRCPRNKFRGPDGECYTRRELKAKAPYFKRLRTNSRAADAAP
                     LGALESKCALPGGKKRVRVCVTQDSKPGASLDGPEAVCRMLKAAENADRESFYVLYLD
                     SKNRVNGIEEAHKGTVASVEVHPREVFKGALAANSTAVILAHNHPSGSPEQSREDILL
                     TRRLVEAGKILGVPVLDHVIVARGGCTSIRGQDESIFDGTESLKNRKPE"
     gene            complement(21369..21797)
                     /locus_tag="AC16V7_13165"
     CDS             complement(21369..21797)
                     /locus_tag="AC16V7_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258117.1"
                     /translation="MAIKDATVSVKLEDIQNLQDSVKALTAEVGSLQEQLTNAKLDAG
                     GDATRQFHAAFLDAMEIVRFAIGNLDPLTVRGWPYQRLAMIAAALKILPGIGGDQVDT
                     ANDLGHFARKCEEWEKARAQGVEQAKLHGENAARGPIVPT"
     gene            complement(21805..22410)
                     /locus_tag="AC16V7_13170"
     CDS             complement(21805..22410)
                     /locus_tag="AC16V7_13170"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR6258118.1"
                     /translation="MITELVECWQRHHQLTVDGFAGPNTLGSILGGAAALAKFYPLRA
                     LTDGRQPVITSGFYTENPSRSTHKGVDFFYKWQEGDPLVAVGDGGAIAKRGTIKWWYP
                     DNTCAVSAADGVVSEAGNTKTGHRVWVDHGHGERTGYFHLQNCLVSIGQRVDAGHPLG
                     LIGDNPSGHDGKHLHFEVSPVGKYAPINPRVWLKGAGYLLP"
     gene            complement(22464..22763)
                     /locus_tag="AC16V7_13175"
     CDS             complement(22464..22763)
                     /locus_tag="AC16V7_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258119.1"
                     /translation="MTPQQLRGPRPIFGILAPGEETTRKFQPLPIGVMPLLQNFPGGS
                     VSLVEQGVDFITVENTGPKRSPFTVLFVTQDQMKQLFLVKSAVDMVSEHRRKKHS"
     gene            complement(22760..22969)
                     /locus_tag="AC16V7_13180"
     CDS             complement(22760..22969)
                     /locus_tag="AC16V7_13180"
                     /inference="COORDINATES: protein motif:HMM:NF014950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6258120.1"
                     /translation="MALRRNPNAPFWVAIEQKEREVIEGALTATGGNITRTAKALGID
                     RSNLVKIMKRVGVDYKSFVTAKAKA"
     gene            complement(23312..23788)
                     /locus_tag="AC16V7_13185"
     CDS             complement(23312..23788)
                     /locus_tag="AC16V7_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258121.1"
                     /translation="MSNDEELFKEFRKRAYSAVDADGNQVIGVKKRSSELTISRLRRC
                     QNCLNFDHEEKAHAYFRHCVERDRATLRDNGCAEDGIREHIQKLESGLRDNFGAVGIC
                     LIRDKRAATEEETPGDFVPFHFQCDSWAGRMIVTAQEAASDPSAAEVYANLKKVDQ"
     gene            23857..25677
                     /locus_tag="AC16V7_13190"
     CDS             23857..25677
                     /locus_tag="AC16V7_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258122.1"
                     /translation="MATSKKKPKEFDEAVLQTLSEPITVTLRKMVAGKGSPIELPPGP
                     GETIGGRGYSADMVRELPSFISNEWSGGGMYDCVATGSNGEQMKWEMYFHPNQVPEIT
                     PPTQINSSQAQNNHQQGPVQQHHQQSGHGGYLSQAAQSYRQTPNQYPFNGQQASGMVP
                     QNNPFGWQNGYEGSSMRFGSSPQSSYTPPTQDSAAAKEREERLKLEAKMERQAQQSQH
                     EKEMAAVTAEMRRLSEHVSKRPSDDESPALKAAMDKIKSMEAQNTTQVLMQQMQAMQE
                     NTNRMFERMAADNDRKIEEMRRAAESNKQDPLIPMLMQVMQQQGTAQQQSMQTFAQFM
                     QANQTQQLEQARLAQSNQMRPQEMLDLFRAGNAGQDQLASAYGKAWELMANGVESILH
                     AQGPGVSPVLSMIGQGVEGTLGMAQQYLEMKQTETQANAQARTIQAQVEAQTRVRTAQ
                     IQAQASAGLRASEASLDDSIEDQEDETDETDEEEIEVIEASSKEQIAAKEVQVFGAAH
                     ENVMRLRKGVASGAIDSSKMAAILLQAIDHFGQAAANGTPLPPIFALFQEERFADLID
                     ALVPSAKVQYREEATQALYAGVENLKRQAGMASAEPQPVA"
     gene            25677..25913
                     /locus_tag="AC16V7_13195"
     CDS             25677..25913
                     /locus_tag="AC16V7_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258123.1"
                     /translation="MIPVVNKCSADIVSVGDTWTCIEISDDATDAGDVELVHVDFNAN
                     VATVRYKGNIHRGPMMIRSDHPSYMGREVAVLFV"
     gene            complement(25910..26299)
                     /locus_tag="AC16V7_13200"
     CDS             complement(25910..26299)
                     /locus_tag="AC16V7_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258124.1"
                     /translation="MPIRYKVKRKYRKHPSGWKQEVGIVATAYDGRKVIGRVGANKAA
                     YSYDIQGANLVEGYLRKGIGSELYRLVAEEACASGMRLRSLDFGRNRLSEGFWKKQLR
                     KGRATRGDRYVEMSCPVPRRLDGSRKI"
     gene            complement(26277..26693)
                     /locus_tag="AC16V7_13205"
     CDS             complement(26277..26693)
                     /locus_tag="AC16V7_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258125.1"
                     /translation="MSLLHAGRDRGATQAALRVGSKWKAAADCEGWPTPKQLGNPARK
                     GEVNYHMFDRYTVAHFATGIGLGVMRLNWWQAAIFAIGWELIETPLKRAIPRAFPYSS
                     EDTLANATGDALGMMAGWAIWQLIVKGSSNANSIQG"
     gene            complement(26843..27247)
                     /locus_tag="AC16V7_13210"
     CDS             complement(26843..27247)
                     /locus_tag="AC16V7_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258126.1"
                     /translation="MKVRNAYGQLMGGSHFDARPTDFDLAKVYGYLPVHRGWISGKLS
                     TAGDLGACCSGCAVGGPCGLGEEIATESDGKPLDPNQREAVARLVAAKLEAEDKALAL
                     RERRSRMLWGAIAGMAAGTTALLAVLQYFKKD"
     gene            complement(27400..27915)
                     /locus_tag="AC16V7_13215"
     CDS             complement(27400..27915)
                     /locus_tag="AC16V7_13215"
                     /inference="COORDINATES: protein motif:HMM:NF039818.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5661 family protein"
                     /protein_id="HIR6258127.1"
                     /translation="MIIGTQIGQEQFICAERYVQVPYGSQRIQDLGYRESIPGGRASG
                     MRPRDFDPGQLAVGTRVELEHTGNARLAREIAMDHLAEDPDYYIKLATIHLDGAQLGG
                     FREDFIGTVGLVMGKEKAAKFLDDFETLVEQKAAAGAQEKVKPMIIGSMAVGGIGLLL
                     GAAALFVAIRR"
     gene            complement(27912..28106)
                     /locus_tag="AC16V7_13220"
     CDS             complement(27912..28106)
                     /locus_tag="AC16V7_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258128.1"
                     /translation="MRRSTRQLSAVSPLTTIFVADTSGKNQKSFLAEVFPVIALMVGV
                     PLIVWYADKAQKRGFRQGAA"
     gene            complement(28103..28237)
                     /locus_tag="AC16V7_13225"
     CDS             complement(28103..28237)
                     /locus_tag="AC16V7_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258129.1"
                     /translation="MPEEEEDSFLDKTIVGPVKVKHALIGSAVAGVGLIGMFMLRGGR
                     "
     gene            28299..28613
                     /locus_tag="AC16V7_13230"
     CDS             28299..28613
                     /locus_tag="AC16V7_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258130.1"
                     /translation="MNEGGGDGATISLDGTSGLVLDVGEIWTDLTMNLFLSSVPGDTG
                     GGRGAGRQNLSISSQVSPFTHPCESVGSALVAATVPVAMASPRSWAASPVAEGDRPPR
                     LV"
     gene            complement(28639..28998)
                     /locus_tag="AC16V7_13235"
     CDS             complement(28639..28998)
                     /locus_tag="AC16V7_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258131.1"
                     /translation="MHSRNNHRLGIITPDMTTTLGFISQEAQRSLGIITPDMTTTLGF
                     GPSRAILGVPLMTWAIVLGASGAGAGVAGFAIGRSAKAAVSSSLGGIVGAAIGIGMAG
                     YMTMREFEKLKSEAVQA"
     gene            complement(29007..29681)
                     /locus_tag="AC16V7_13240"
     CDS             complement(29007..29681)
                     /locus_tag="AC16V7_13240"
                     /inference="COORDINATES: protein motif:HMM:NF013961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HIR6258132.1"
                     /translation="MDNRKLLYLGLLGLGIGAVGAGAYYYTKKKRTTPLGRIADSLET
                     EDGYKLTHHRQDDLPIKDRVAILQDLTAKSVKDPRMRKIALQMTSKCKERDSNCEAKS
                     IYNWIKKNVRYTGDIGPHALWAGGPVEGVDLFQTAYRTVEFGGGDCDDHAILACTSAL
                     LNGMHCKYRVTSPRKGKNEDYTHIYAMIGQPKMRPSKYIAVDTTLPGSRYGIEAPHAK
                     RLDFVA"
     gene            29747..30508
                     /locus_tag="AC16V7_13245"
     CDS             29747..30508
                     /locus_tag="AC16V7_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258133.1"
                     /translation="MLIAAENSSGVQLVGSGFGDFAGTASVQQLQEALLSLSMAAGWT
                     AANPGPITGDVSLQTISAIAAVVAKLGGKISSSVKTAVQLAAVGAAFDSTLRGAAQVA
                     IAAHANELRGGVLALTAIYTKSSAPPIPPAPPAKPLLITQFMMKSTTPSAAQTAPLPP
                     GSVQAQTKAGIYRVAVPASQAVGLGGPSVSLGEAFKELPARQSFTPGVILVSEGDLKK
                     KTGEAKPFWKKPMYWAIVGGVVVVGGGAYTMIRRR"
     gene            30510..30965
                     /locus_tag="AC16V7_13250"
     CDS             30510..30965
                     /locus_tag="AC16V7_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258134.1"
                     /translation="MILDRSKSPFVPVVMPSNFTEEDSVYPLFGADFGETEEEAILRK
                     RDELFQKLRAEADAEAGAEEAKQVASRKILIFGLAGVAIGSLVTGGMTALSLRAYSRS
                     GSVMGPAIYAGIGSAAMGTAMILLLSKIGAGKTVDPRAAALAVGARMAA"
     gene            30966..31142
                     /locus_tag="AC16V7_13255"
     CDS             30966..31142
                     /locus_tag="AC16V7_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258135.1"
                     /translation="MARKLPKKFLACGALYQNYRANKDDNVYAACRIVKKANRSKCKW
                     SAKPGVIRSCKLRY"
     gene            31150..31563
                     /locus_tag="AC16V7_13260"
     CDS             31150..31563
                     /locus_tag="AC16V7_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258136.1"
                     /translation="MSKRCEIRQIDMGGKNEFFFELVCNGKRQAAIHAAKKKIAGKVR
                     FVVRGVNVLEQFRRKGLGTKLYEMAAQEACRRRAPLASDERIGDMSREFWAKQIRKGR
                     ATVLSKKGGSEKGERAPVYSLTCPAPPSLRGRVKK"
     gene            31560..31802
                     /locus_tag="AC16V7_13265"
     CDS             31560..31802
                     /locus_tag="AC16V7_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258137.1"
                     /translation="MKDIFTGGKGTIAGIHWAVWLALPVVSAAGGAITSGPGRRGYSA
                     LGAAGGSLIALTAFAILEMQPSGPRPDPEDLKSLLR"
     gene            complement(31799..32071)
                     /locus_tag="AC16V7_13270"
     CDS             complement(31799..32071)
                     /locus_tag="AC16V7_13270"
                     /inference="COORDINATES: protein motif:HMM:NF024139.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR6258138.1"
                     /translation="MTKNKKEESRSHEPLPRKVKIEEKRACMVATNGDGSVVPLMSAR
                     EISDYLGIGRSTLRRYVHSGIVPSIRIGGHVKFDVLAVRAALDRNS"
     gene            32212..32715
                     /locus_tag="AC16V7_13275"
     CDS             32212..32715
                     /locus_tag="AC16V7_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258139.1"
                     /translation="MSMKHVHVMNIRPDVITRTGVIQPYAIREIPPLAVARNSVQAFE
                     QVARPSGPYVQSEPQPEEVAALAKRLATAMAQGVRPPAGTSPKLIQAARYIAEGKPFA
                     IKSAGRTAQVKASPGYAGRKNTVVPAVRGRANTVVPAVRGRANTVVAAVRGGRSNTVV
                     RSVRGKR"
     gene            32922..33467
                     /locus_tag="AC16V7_13280"
     CDS             32922..33467
                     /locus_tag="AC16V7_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258140.1"
                     /translation="MKHNNDNLGLGGVDAFGLDEMGQPVGLNPVWGAVVGTGLGTIGA
                     IATRQFSPKLAKHSELVGFGLASVASGAMIAFEGTRAAGWTGLASAFLNNGLRHMEKM
                     FAEKSIEGSVVPNPLQGVVIDPTEALGLVEINPTYALGEGNDMPTLVGANLASASDHV
                     QLVGGPSLAQTAGHWGATVMG"
     gene            33561..34253
                     /locus_tag="AC16V7_13285"
     CDS             33561..34253
                     /locus_tag="AC16V7_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258141.1"
                     /translation="MGKVGIAPGTTTLTLPGGQTIALSDWIDDKHWTMVELENGDNDT
                     LDAFASGRSQQITGGTRANTLVDTNVPRNGSIGLPKDWEFMVYGWAMEVIRVTRPTGQ
                     ETSPRLTSFSDPASLRTLFELDRRLFCKYIYNGKMYTEGMFRDYPQGHGISLFTTASS
                     TELAQNGIPSPRDRVALVLPVHERENLGYSFEISPVIALAIAQPASDDGAVLNFLDLR
                     ITKNGLIKRTVV"
     gene            34345..34845
                     /locus_tag="AC16V7_13290"
     CDS             34345..34845
                     /locus_tag="AC16V7_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258142.1"
                     /translation="MGRNSIIPHSFADQVITFRIPYSMPGELVVEPNASGVFFPEATF
                     LTNVDKPFEIDRMYVRLTALDENKDVLDVQPGTLGRRVRLSIGDTSKNEKLTKSAHIV
                     DTLISSEAGAAGSWEWYVPYTMVRSEGFQIGIDTLAMPAGTDFVRVEIAFQGSLIVIQ
                     PASETR"
     gene            34853..35170
                     /locus_tag="AC16V7_13295"
     CDS             34853..35170
                     /locus_tag="AC16V7_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258143.1"
                     /translation="MQDSTFQTFSVPGASTAATIPLTIARGPSDSAVRVVVKNVGAVP
                     IFLAGASADAVSPEGPSSNTFQLDPALSEVFVLAARQLMYAVGSVPGGRVSVHVSGEF
                     RRL"
     gene            35369..36172
                     /locus_tag="AC16V7_13300"
     CDS             35369..36172
                     /locus_tag="AC16V7_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258144.1"
                     /translation="MLEDFRVYTGALSTYDPIVFDSKIDLIGSNDNPVPPDTTGSGSG
                     QTAGGAMATHQSQVSVHAPLIKPISSGTAIVARNQSRYNEHYFDESGNDQDFPSTRTR
                     EPVLVLRCYIRSPGPLSYPSSRAPLLVNVRQAAVVLDKDNNPVQYGLTDTFVAYAIPV
                     GGRKRFHISLWSLPDQFSGADSGVINVRASVLRGGETNRENLFNTDWAGNFFEEEVIP
                     STALTPGGATNKVRYEELHNSAEWLLVFVEQTTLVANVCDVSVVMRAED"
     gene            36172..37119
                     /locus_tag="AC16V7_13305"
     CDS             36172..37119
                     /locus_tag="AC16V7_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258145.1"
                     /translation="MRYDVFPLVSDLKGRFALGEFTPPKFIPDPRKSDISARIFIEIW
                     IAWAADREQKIREWATTIDNLIATKSATCAAIREYNDAAIALYVREVEMGERFILGGI
                     SRSDLPPPFFPLVFASSTRTKSINFKLLDWPTGRLTIEYDLPCPPVDPFGPNPDPRLV
                     FPDFSKLRVFGDPRDVASVKTPSGLGALQAVGWAAVVLGAALGATAVLSIREAFRGVN
                     NTELAEINGEIVDKNFDADFNRANENRDCVDDSLRKLGRELTVSELLSIRTECARIAD
                     GLFPRAETIAEPKSFGFALLLGGVGIAAIVGSIAYFRRE"
     gene            37390..37671
                     /locus_tag="AC16V7_13310"
     CDS             37390..37671
                     /locus_tag="AC16V7_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258146.1"
                     /translation="MAKSRKKANTPQAKRFGVIVKAANVVCHRDTNSVNAYKKCMSSA
                     MKAKLPTGGKKKNPLLKCKGTYSSGPNKGKVKPGYRRGAAGRCPVKKKK"
     gene            37751..38056
                     /locus_tag="AC16V7_13315"
     CDS             37751..38056
                     /locus_tag="AC16V7_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258147.1"
                     /translation="MSLNKLYSIVSFVDAPSGVTSLPHGLNQGGTPVTPDELKVSMAL
                     EEGSVTADETNIIFNNDTEGTVSITVLAERWHTIERELGVQDPAVNLSIQPWVTYSA"
     gene            complement(38060..38752)
                     /locus_tag="AC16V7_13320"
     CDS             complement(38060..38752)
                     /locus_tag="AC16V7_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258148.1"
                     /translation="MTQSIPGEKLLGMLNVQLYGDDDLGWPGFVDDAIDWVVDKASDA
                     GGAIQDGASWVVDKACATARDPKFMAATAGAAAIPTGVSQAAAGIAAGTSAACMILVP
                     PGGPSIPAPPVKTFPLPTLVLERQMMFRPPAPPAPPALPAGTIAAFDAKRGGYRVAVP
                     SGLAGFAAAASFTEVGTQASLPKGARLVDLVTFKKDTGNEPPWFKRPMVLGGIGLGVL
                     VLAGGGYMALRR"
     gene            38790..39263
                     /locus_tag="AC16V7_13325"
     CDS             38790..39263
                     /locus_tag="AC16V7_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258149.1"
                     /translation="MSDEILQRISGDVSELQDDMGQVKERLVAHDTRFGNGIHVMGEM
                     KGRIEKVEGKIEPKAPDWLKMMLAGLSVVGILMGAQLWLTDRFNDRPTQAEVEKLTAP
                     IKAAQKETAKDVGNIEKSQSAQQTSIKNIETTQATQGGKIDKILERLPARRGERE"
     gene            complement(39264..39848)
                     /locus_tag="AC16V7_13330"
     CDS             complement(39264..39848)
                     /locus_tag="AC16V7_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258150.1"
                     /translation="MAKAKSKPCVPTREKTTGAILKAKIGDPNWNASPVAMKTPGGGK
                     GRKIAPDYITGEVVPGPKSITTTEKCSLTEKGRKIKIKDCDTSLQFVGKTTSPVMGVP
                     AGAYLRICTKFGADPIMIPVRDHADATKKSRKICGCVTSGETSMKCAKKVSGAVRPKL
                     RRQAQKTSSRAGEKRDCLEWSRNGKRCLRRAKKK"
     gene            complement(39858..41219)
                     /locus_tag="AC16V7_13335"
     CDS             complement(39858..41219)
                     /locus_tag="AC16V7_13335"
                     /inference="COORDINATES: protein motif:HMM:NF036314.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GP88 family protein"
                     /protein_id="HIR6258151.1"
                     /translation="MSAITIHIEQHVDDDPEDRNAVMQCPTGVTLEGPRQCHPDLEQT
                     HNGLTRSVWLRRTIEDLLNGREVTVVDGRTAGKGGEGAAGLYFKLNPPILQHDILWTS
                     GTRDEEYISFFTENSKMACPNWDLPAGSPLVGGSCPGATAGQTIVPLEVREKINVQAP
                     TGYGSAEGLYTGTPSSAQKKKLAVVNPFAFDGDLGRQPVDRVGPVGMPVRVTDTICQI
                     CYAEGGQYATLGPQLREMVCYWWTREMLKTTEGTEEWIRTMVRAISGLSWGHEEQIDP
                     RTGKQIYPVRVHSAGDFFSPRYAEAWLHVAARLPEIFFWAPTRTWAAQGWMGTTKNGK
                     WVPGHWAHMMERVGIPNDNFSIRPSAYHTNDAAPGPEDYPWDGPYQFPTAGTTSLYAF
                     ADATEPEDCGRSQDTRYDWACQTYAIGISSPSCKNALAPNNVIGCRACWLNPELRINY
                     TTH"
     gene            complement(41260..41514)
                     /locus_tag="AC16V7_13340"
     CDS             complement(41260..41514)
                     /locus_tag="AC16V7_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258152.1"
                     /translation="MAYYEDGGIVCAKFENPRKSFDKGSFRWTAKTGTRESGGVTRVL
                     MGCPYGAWNAQTKRCRVGTKAHKIYLFSNKKRCPAGYRKQ"
     gene            complement(41515..41973)
                     /locus_tag="AC16V7_13345"
     CDS             complement(41515..41973)
                     /locus_tag="AC16V7_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258153.1"
                     /translation="MQIQSLGAVDYMIPELDPNYLGDHLGGELEDAGRDLALSKSLTA
                     EELASVLRPQAPSSRDVIAASFVANGGDVALLNAALGMLTGFGPGSPARRNFSLAWKV
                     FGTASMAAGVYHGYKRNQSVGWALWWGLMGGMFPVVVPVIAVAQGFGKTK"
     gene            complement(41983..42324)
                     /locus_tag="AC16V7_13350"
     CDS             complement(41983..42324)
                     /locus_tag="AC16V7_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258154.1"
                     /translation="MQVTLVGSSLQGFGETQAELIALGKSLAVRVLNAGFLAQTLEKL
                     SAADQDVVAASFIAAGGSAIELAEARATVARRSGNTPNDGMRRKHIILAAASGGAGLV
                     LGVIVGRMTKR"
     gene            complement(42344..42652)
                     /locus_tag="AC16V7_13355"
     CDS             complement(42344..42652)
                     /locus_tag="AC16V7_13355"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012314.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR6258155.1"
                     /translation="MKIWDMPVAILVWTQDGCPACDEFMPKFSKIAQEYAGCLPIIIA
                     DVSQFPRAADTFRVQSTPSTIITRYGRRGFAGFIDGDASEERIRSLFSAAANGLDCQV
                     "
BASE COUNT         9197 a        12310 c        12548 g         8767 t
ORIGIN      
        1 gccagccttg aagtgtccga gcgcgacttg cacgacggag aaatcagggc cgagtggcca
       61 gaggatatcg ccgagtgcat tctactggcc gtcgactacc acctcgaggg gtgcgaaaaa
      121 acaaccaagc gatacacctc gatgcgttcg cttagcaacc ggctcatcgg agcccttaat
      181 ggagtcgagc aatgagtgca atcccagaag tctttacgaa gtcacagatc aacgaagagg
      241 tcgtatccgc tgcagctcgg ctgctggagt tgcgagctga gttgctgcgc acggccgcca
      301 gaatgagggc cctgggcgag cgcttggtca tggcccctcg ctggaattac agcggcgata
      361 tcgatgccgt tgccgttaac aagctcttcg ctggagagga tgaggcgcag gccctcggcc
      421 acctttcggt gctgctcgat gtgctcgccg accataccaa ggctgcagtt gcagagatat
      481 cgtccgacgc gaaattccgg ggtgagtcgt gaaactggaa atacgcacat ttcaaattga
      541 gcacaaaggc gtccagcagc tttggcttcg catggaatcg catcgcaccg tgttggagtt
      601 tgcgacttcc gtcgagggac tccagcgact ccacacccag atcggaattt cgcttgaatc
      661 tgcacgcgct ggtttcatgg tgcaggcaac cgacgtgggg gaagtatgag tgacgaccca
      721 gtagaataca aagtgcgcag cccagagccg ctcgcccagg tggagcccga agtcgactat
      781 taccggcgta aactgcgcca gctccgagct tggctcgaag agctggacca ggctggcccc
      841 gaagatctag gccgagcgct aatgtcgtcg cacagcaaga tccgccaggg agtcatcgat
      901 ctcctcgacc ggccgcgcag caacgggccg gcgattgacc gtctcgagcg agagctcgga
      961 gagctgcgag gaaagttgat cgacttcgat atcatgcgca aggagcttgc ggcggccaag
     1021 gaggcggccc agctctggca gcgaagggcc gagggtggcc cggtgaagac tgcggtggtg
     1081 tcggccgctg gtcgcctgga ccggtggcta gggagaaaat gaggcaaacg agcatagacg
     1141 catgggagga tataaaggcc tccggagtcc tcggtcttga gcagctaagg gcttacgagt
     1201 gtctgtttcg ccacggaccc ctaacgggca gagaaatcga cgataggctt ggcgtcgaca
     1261 tgcacaagag gctttccgag ctagagcgcc tgggggttgt caaatgcaat ggaaagcgcg
     1321 agtgccgcgt tacgggccgc caggtccatg agtgggacgt gacaccttat agggcaacta
     1381 ggatagccaa gcgtgccgcc aggaccggta caaaaaacaa gtcgacgacc aaagcgctgc
     1441 tagagcttct caaggaggca tgcaaagccc tcgatgagag cggagacttg tttgcgcgga
     1501 gcaaggcgca tgagattaga cgaaaggttg gagagctatg aaaaaacatg acgaaacctg
     1561 cgctacgtgt ggccgcctcg gccatccaat agcagagact tggatcaatg ggtcagaggg
     1621 gccgcggtgg tgccccgagt gcaacgcaag aaagaatgaa gcgttctcga aacggcagat
     1681 tctagtacca gatgcacact acagcgatcc tgggcacctg cccaaggaca cctcagtact
     1741 cctggccgtt ggccagcagg caatataccc atccggcccg agcgtactcc tgacaataat
     1801 ctggggtagc ctcggcgagc gtcgaacgct tgagatccaa ctcagagaag atgaaataga
     1861 tgcgttgatg agtaggatct ctgtgtgccg agattttagg tagccatgag ggacggcata
     1921 gacgatggac tggaagctct cctcaggatg caagaaatct ggccccggcc acccggtggc
     1981 agcgcggcat ggtctggctg ccaactcgta atttctattt tctccagtct gcctgcacta
     2041 gcagatgagc ttggggtaac gatggatacg atgacggtag acgacatagt tgtagccatg
     2101 aagagttcag ccgagagagg cctcaagccc tgatgcccac gatctacctt atcggcggcc
     2161 caaagctaat ggggtcgacg atgcgactgc gctgcgcctt gctgagcttc ttgatgactg
     2221 gcctctgcca gctgcacttg aggtcgtgtg ccatgcccag cattcgctcg gcaccagcac
     2281 gatcctgaag gccactggag aatgacttgt acgatttttt tagaccccga cacgcgagtg
     2341 ccttcgtcct gtgacccaac cacctagcct cgaattcttt agccatgctt ctaaccaatc
     2401 cagctcttgt aagccttaac ggcagccttc acggccagca tctttgtctt cttgttgccg
     2461 tggagagtgt gcgggctcgg accaacgctc gagtagcccc aggtgccgtt gtcgcgcttg
     2521 cacacccttg caatcgggta catcgcataa ggactgcgca ccatgacggc ctcacacttg
     2581 ctggcgttct tagggtccgg aatgtagctg ggcgtcagga agagcttttt cttggccatg
     2641 cacagagcat agcccacagc aatagctagc tgcagctact caacgctttg cctcagataa
     2701 tccatagcca cggctttacc ctggacaccc taccgatcag aaggtatgtt ctgatcggta
     2761 gggtaagact atagaagacc ccataaggag tgaatatgaa agcgagatac ctttttctgg
     2821 gcacagccct gctgagcggc tgcagcttgc tgtttgaacc taccggaggc accgatgcag
     2881 ccagcgatag ctttggcggt gatgcttccc tccccgcaga tggtggctct atagacatcg
     2941 acgcaatggc tgtggccgcc gacgccgcgc tttgcgaccc cttcacggat cctgactgct
     3001 acgtcgacaa ctatcctttg ggcggcccat accctcgccc aggtggagct ggcgtcgatg
     3061 gcagcgactg cacgctatcg agcgagtgtg ccgcgagcat gttctgcact ggcgtggcgg
     3121 gcaccgaggg ctattgcttg ccgctctgcg atgccgagca ccagtgtgga ggcgaccggg
     3181 cctgcctgag taccgattgg cagagctacg gattttgcga gttactttaa cgaaaggatc
     3241 taggcatggc ggcgacaaaa ccagaggcga gcaagccaat gagccaactc gacctattcg
     3301 ggcagccggt agcagacccc gaggaaagcc caaaaccgcc cgccaagccc cgtccagcgc
     3361 caaaaccaaa gcccagtccc cccgaatgtc cggtgcgcgt ccagggtcca gctgacgagc
     3421 cctgcgtgcg ctgccgggag cgattcgagc accacttcgc tggagctctg ctctgcgccg
     3481 cctgcgcgga tgcgctcgtc gatattcagc tcgaggaccg ttgcatcgtg atcggctgcg
     3541 gctgcgtgac gatgggcgac gagatcgacg gccacccgat ctgcgggctg cacaacaccg
     3601 acgtgaccag ggcaattatg gcgaccggcg catggccagc ctggtatggc tacgccggca
     3661 aatggattgt ggtcgccacc gtgcgccgac ttgacgacat gggccacacc tgggcgggga
     3721 tagcggccga caagcactgc ggggcgtgcg gcctgcccta cgccacctgg acgaaggatc
     3781 gattcaccgc ggcttgcacc gactacgcgc agccggatac gacgaaagga cttttcagac
     3841 catgatagag cgaccgatcc tattcagtgc ggaaatggtg cgggccgtac tcagaggcga
     3901 caagacgcaa acgcgacgag tcgtaaagcc atcaaagaaa agcgagtggc tgttctcggc
     3961 cgactgggcc gacagcttcg tgatcgcacc tggaaacaat gcccttgagt attgtcccta
     4021 cgggcaggtt ggagaccagc tctgggtgcg cgagacattc tgccaggtcg acgacaccga
     4081 tcatggcggc gaattgtggg tcgactatcg cgccacaccc aagtacagcg aggagcaccc
     4141 agcggggtgg gagaacgcac catgcgacca tgaggctctc aagtggaagc catctatttt
     4201 catgcccagg gcgctatctc gtatccaact cgatgtccta agcgttcgtg tggagcgact
     4261 gcgcgatatc agcgtgcaag acattgtcga cgagggaatc aacgcagagg tcagagtcct
     4321 cgaccacgac gttgcggagg gaacggttgc gcaagttttg aagcacagat tcatggagct
     4381 gtgggatggc atcaacgcca agagaggcta cggctgggac accaatccgt gggtctgggc
     4441 cgtcgagttt ggagtgatag aatgagcacc aaagcagaaa agaagcgacg gcagttcgcc
     4501 gagagagagc ggaggcgcaa gatcgaagag gagctgtcca agaattggat ctgcgaccac
     4561 tgcaagatca tggtgcagca ctggtcgcct atctgccttt gcggccgttt gcggccagag
     4621 gtgaagctat gaaacgcatc ggaacaatcc cagatctcta cgcagctgcc cattttatag
     4681 cgaaaagctc aacatcgcag caatacgtgg atacattccg tgatctggaa acgtgcagcg
     4741 acgagcgtgc acgagatctg attcgcctgc taggagtaac aagtagggag gaggccgaag
     4801 aatatctaaa tatgatgatg attacctacg tggcctctac gtcgtggatt tcgcaagggt
     4861 gtcagttgtt ttcgctggac gagcgcacga tggatctagc tgtagctacc tttcctgtgt
     4921 cgctcaaaga gtttcacatg gggaacgatc cggaggaaag caaagatgag tggaggcttc
     4981 catacgaaac attcgctatc gagtttccgt ttctggtcgc tgtcgatatg cacaactcag
     5041 aagtgaaagc gcgaagattg attgtggagc ctttcagaaa cgtccacgac gaattcgcct
     5101 ggaatgtctt atggaccgac gtgaccaacg acatgagcgt atccgtcgta acagaaggga
     5161 ccgtgctgta ctccctaatc acaaacctat gcttttttct aaacgagaca ggtacgatca
     5221 caaggacaag gccctcctat ggctttacgg gaagagccaa gtcagccgcc cgtgccgtga
     5281 aggcgtggtc tgcatcgttt caccaagtca ccaatctgcc gctgggcata gctaaggagt
     5341 ttcttgcatc aggtaagccg cctggcgagc aattcagaat gggcaagcgg atcatggtcc
     5401 gcgggcactg gcgattgcag ccctgtgggc cgggacgcca attccgaaag cataagtgga
     5461 tcgctcccta catgaagggc gacgaggacg acccattatg gacgcgaacc tacaaggtaa
     5521 ctccggggaa gttagagcta tgagtatcgg tggagaattg ttcgtgtgcg tcaggtgcct
     5581 gcaccttctt aggccagggg agccggtgga ccactttgag cccgactggt tcgactatgg
     5641 gggctcagcg tcccgctgca cttctcatgg tgcgtgcgac gagcgcgtca aagagcgcag
     5701 agctcagcac cgagaggaaa tcgagcgcag agtaaggcac ctaaaagaag gggagtgcct
     5761 gctggtcatt caagaccaat ttggagccaa atacgacagc tatggaaact actacgttac
     5821 ggtcgcagaa gaggacgccc aggacgagtg ggataagcgc caagaggagg taaaggagaa
     5881 tggcgaagag ggctgcctgg ttctgagcga aacgaggaga caatgacgtt ttacttttgc
     5941 aactaccgcg tgatgtttga tgcgttccat ggaccactac ctattctgtg tggcaaagag
     6001 cgcggccacg acgggcatca cgggtcgtgg actcacgtca atgggaggcc aaccacctga
     6061 gggacgacga tgcagatcag gatatgagct ggcttgatag gcgtgatcaa tgaagtgcct
     6121 gtgcggtggc gacacctcgg tatcggacag ccgaacagtt ccgaacaatc cccattcgac
     6181 caggaggcgc agagagtgcc tagcctgcgg caagagattt accacctacg aggtcatgga
     6241 ttcgatggcg acaattacag ttaggctgcg cagcggccga atagttattg agcacagcaa
     6301 aaaggacgag aagtgagtga tcaagaaata atatcaagat gcccagagtg cggagacacg
     6361 gccctgcagg agtctcacgg gctattctct acctgccatg tttgccatga gagctactct
     6421 ccgcactcag aggagatggg cttcgagcgc acctcctggg aggagtgggc cagttctgtg
     6481 tatagcgatg acagaaaggc aagttgcacc atggatagcc acagagccct atcgtcggcg
     6541 accatcgaca ttttcagggc agcgaccata aatcacaagc cgtgggtcga tgaggccgag
     6601 ggtatcaaca tggagcttgg agactgccca tcttgcggat ctagcctttg ctggtcattt
     6661 gacgaggtgc tagcttcgcc caccatggaa atgcgagtag aagagctgga ggcgtggctg
     6721 agtcaccata agctggggct gtcggtgtcg ttcaatgagc ggtgctatag cgccgagttg
     6781 cgcaccaaga tcggcaacga gtcaatcgtt gcgatggagg ccaaggcggc cgagtttgga
     6841 gtggccgtca ttgcggcctg tcagaactac gaaagggaat atgcccagcg cattgactga
     6901 ggtgatcgag aggtcgaccc gcctgggcga gaagtcaaag gagaagtaca tgctctgcgt
     6961 ggagcgcttt gtcgactttg ccggtgaaga tcccgtaggg tggagcggag ctgcggtgga
     7021 gcgctggcga gaccacctga gggctgacaa aggagctggc accgtcaatg gtcatctcta
     7081 cgccctccgc tacgcgagtc gccgcctgga gggtctggga ataggtgtcg acttcgccag
     7141 ggccgccgag tcgctaccag atccaaagac tcgtaagcgc aagtcgatga gccttgagga
     7201 ggtcaaggcg cttatagaga cttgcgaccg ccagcgccca atggatctgc gggaccgagc
     7261 aatcatagta attgcgctgc gcactggcct gcgagcatcc aacctggttg ggctgtcgtg
     7321 gggcggcatc aagggtcgtg ttgccgactg cgttatcaag gggaacaagc accaccaggt
     7381 catactagac gaccgctgcc ttggcgagct gcaacggtgg ggagattgcc tggagtccag
     7441 cggcaggagc ttgcgcggcc caattatgcg gcgcgtgagc gagctcatca cgggcgacta
     7501 ccacatcggg aacccgatca agcggagagc ttgggtcaac gaaatgctcc gctcgagatg
     7561 cgcggcggcc gggatcagaa acgtccacgc gcaccttttt cgccacacgt tcgtgtcctg
     7621 ggcgcttgag gctggagtag ctccgcaccg agtcatgcgc cagaccggac actcgaacat
     7681 ggccacactt tcacaatacg tcacagacct ggaagctgag accaatccaa tcggcgacca
     7741 cctaccggag ttttaatgag acacaaagac gaaccagaag aaacggatct cgacgagacc
     7801 atctgcgctc tccacgtcgc cctgcgcgag gtagcattcc atacgatggc agctcagggt
     7861 gccagggatg gcctcgaaga ttgctttttg cacatgggcg acccagaaat atcggagtcg
     7921 gctgacgttg ccctatgggc taacagggtg tcgatggcgt atcaggccgt cgcagatgtg
     7981 gcgtactcag tgtccatcga ggcaggcatc gtggcccgca ttatggaacc aaccgacaag
     8041 gaaacggaga actgatgggc atgaaaaggc gagtcgagcc agaagcgcga gaggtgatct
     8101 tgcaagtgcg gccggtggct gatgccgagc acccgtggtg gcctaagctg gaggggtggt
     8161 acagatgggc atcaatcgat ccgtatgacc caccagggtg gtcgatgggc ctcgacgaag
     8221 attacgccat agactctatc gagctgtgga aacagacaaa gacaaccctg gaccagcaca
     8281 tcgagcacat gaagtctgag tggctcgagg agtgcggccc agacgaagag ggcgacgggt
     8341 ttctttggga gttcagggtc gtcaaccgct aatgtggagc catgctcaag tggatcgcgt
     8401 tcaggctgtg ccagctccga agatggagaa gcaggaatac gtgctatcgc tgtgggcact
     8461 ccctggctcg tcacgagcag caaattggcg gtgactggta ctgtgatgcc ggtggttgcc
     8521 tgtgctcgtt cacgagctac tttgggggct agtactcccc gtcagtggac ttcataaggg
     8581 taacgccggc gagagctctg gagagctgca tcgccttgcc aggcgagagg tgaacggaaa
     8641 tccgctcgcg cactggacct tcgcctccct cgttgaagct cagtatcacc tcggcgtttg
     8701 gccccatatt gataacgtgc agagcccatc cgtcgtcgag cgggatcttg atcttagtgg
     8761 tgagtgtggc cactgggtgc tagcgacgga caggcgtgtc gcggacacgg cacctgggtc
     8821 gcttcttggc cgaccctaga ccagacgtga ggcttttgaa aagcctgtag gcctcatccc
     8881 ggcgagtgaa gtcgtagtcg caccagctgt gcgagacaac gtatccgctc ggcgtcgacc
     8941 gaaccacaac gaccccctta gaggagtgcg ttttgacgga ctgaggcttc ttgcgatgtg
     9001 ggaagtactt gtcgattgcc atgttagtct ctcctcgcca cgagcacggc cgcagcaccg
     9061 ccggccagga ggaacgcggc caggacgccc acaaatacgc cggtcgacat tttctttctc
     9121 tcatcggccg tcgaaacaaa ccgctgcgag accggcgtga tggcgggccg caccaggggt
     9181 atattggact tgatgtcagg caactgaagc ctcgttggcg caggcgaagg agcgacaggt
     9241 ggcagcggag caactgcggc aaccggcaaa gctggggctt tatcgcggcg cactgcggcc
     9301 tcttgcaggg tgcccaggac gttgcgggcg tctttggcta ccccggctgc ctggccggct
     9361 ttcgcctcag gagataggtt gcgctgcaga tccgggtcga gtcgattgac gacgagcttg
     9421 agggccgagc cagtggcatt gcccatcttc ccgtcgaccg tcaagctgag cggcatgccc
     9481 atcagctgac cgacctggtt gatcgagacc tgaatgctct taaacatggc caggaccgga
     9541 gctgtaagcg ccagggcgac cgggccagac ctggtggtgc ccatgcgata atctgagccc
     9601 tcacgtaggt tgggcatgaa gccaagcatt gggtcgatgg caccaaagcc atccatactg
     9661 atcgaaagca tttctctgtc tcctactttt tccgctcaag ttcgtactga ataaggatcg
     9721 ccctcttcgc gttggcggct tcgagccaga gcttttcgtt ctcctcgacc tgcatcactt
     9781 gctcgagggc ctttagattc atgctgtgga tgtattccag gcgtccacgc ttgtcctcgt
     9841 tgaggtagct gtagactatc ggcagcacct agcaccttct cttgttgacg acacagcgca
     9901 tgaatcgccg gctggcctcc tcgacctgat cctcggcctt gccaaccatc cgccacagac
     9961 gagttgttct gccggccctt tttgcactgt gcgggccgat agatgagagg tgtacgcgag
    10021 cctgggcaag attgcgattg gcctcgacga gcctgtctag gccctgcttt ccgcagccgt
    10081 agtcacccct cgaaagcctg atggcttcct ttgtgccagt ggccgcaaag tcctcgtgcc
    10141 acctgcgtgg gaatgctgtc accttgggct tggccatttt acttaccctt gagcatgcac
    10201 ttggccacgc ggttgccggc gaggtcaatt tgacgctcgc gcttgttcac gaatgcccat
    10261 agacgccgag tcctggggct cgacgagcca aagccgatag aggctaggtg tgttctggcg
    10321 tgagcgatat ttcggttcgc ccgcaccaga gcatagacgg catcgggacc gcagttgcgg
    10381 gtccacatca actcgttcgc cgccctcttg gcactcgaca ccgccatctc ctcgtgccac
    10441 tcacgagtca taacggtgtg ctttggtttc cgcttgggca ttcctatttg aaggccatgg
    10501 ccttacccaa caggtaagcc gtggctgcag ttgccaggag accggcagcg gtgccaagaa
    10561 gcatattccg agaccagatg cctttttccg gcagaagccc gaagtcgccg acgagggtca
    10621 cgtcgtcata ggcacccatc ccggcaccat acatggggcc gtagtccggg taggctccgt
    10681 tgacaacgta gccaggctca atctcgacga agcccatgtc gccaccgacg agggtcacgt
    10741 cgtcataggc acccaagctg ccattgattc cgtagccggg tccaactgtg actgctgata
    10801 aactcatagc gctatcctga aagtccccac gacggtctaa agcagaagca ccaagggata
    10861 tggccgccgc ggggcaaagc aggccgtttc gctacgagct tcgatagcgg aaaactactt
    10921 agcgcggcgc atgcagcgcg tgcggccctt ggaccacttg aggcatcggc ccttggcggc
    10981 ttttttggtg ccagactttt tgcgagtctt ggtgccactg aattttcgac aaactctttt
    11041 gccgcccgac tttccgaact tcacgcattt tcgtgccatt tgagattgat cctaatctga
    11101 aaacgatggg gcgacgcgcc cgtaactcca gaaggctacc acgactcaat ctgagaatgg
    11161 aaatagctaa ggtccagcaa tatcgacggt ctacccaaat aggtcgatct gaacaccccc
    11221 ggagatcctc gaactggcgc gggcgtgcgt ttcgggctcc acctcggagc caatgaatcg
    11281 gcggcccaga gactcgcagg cgacagcggt ggtgcccccg ccggcgaacg gatcgcagac
    11341 gaggtcgcct gggcgagagt agtcccgcac aagtgcgctc attaaccaga ttggcttttg
    11401 ccccatgacg atagacttct cgccacgaga tgatgccctc gacgggggcg gcccgacgta
    11461 ggcccctggt agcgtgcccc agctcataaa ctccttgcgt cgcggccgag cgacgaccag
    11521 ccagcaagtc cagctcgagg gtccgtcgcc ctgcagccgc acgctcatgc cgctcattac
    11581 gcatgggact ggtgggaaca catagcgacc ggcggcgagc agtgattctt cccagtgagg
    11641 catcaagtcg tgcgacgtgt gaaagcacat ccagcccttg acccttggcg accaggaggc
    11701 gcagagctcg gcaatgtcct cgggcgtgaa agcatcgtag ccgattgcaa cagagcgctc
    11761 ggcgtcgtcc ttgccttcga ttagtgcgcc agctcggtgg acgcgagcgg agtaaggagg
    11821 gtctgatatc agcgtgtcca cctcatccac atcggcgaga gctgtcctcc agtcgcccag
    11881 tcttaggtct gccatttagc gcagtctagt ggtgcttgac ctgttagtcc agcactgata
    11941 gacatactcg gtgggtaaat ccaaaccgaa gagcggcgca gttctgctcg ctacctggct
    12001 cgaaaagaac aaggtcacac tggccgcgtt cgccgctgac gctggctgca gcaagcaggc
    12061 cgttggagcg attcggagtg gggcattcaa gccaggcctg gccctggcgg ttcgcattga
    12121 gcgagcaaca aagggaaagg tgccgacgat atcgtgggcc taacgagaaa acatgagccc
    12181 agaagacaaa gacctaattt gccgaatggc agcggacatc gcagggccat tattggcgca
    12241 ggcagcagcg cacccgcgag gaggtgggct ggacgtagag ctactaatga actcggaagc
    12301 atgcgcgtcg atgcaccgaa ccgtggcgaa acacgcgctt gcggcctcct ggcaaatcat
    12361 caacgagata gagtcgaagc tcgaggaaaa gctctcccca cccgaatagg gacaacgaac
    12421 ggggaagtca gtcggtcgta ttgctcggca cttggatggc aattggtccc agagaccgag
    12481 tcacgccacc gagcctcgaa atgcctaacc gagtgatata gtctcactgc atgctctcag
    12541 caacgcagat cggcaatgta cccgcagaag tgaagtcggc ccagcgcgag ctcttggccc
    12601 ttgcggagga ggtacgcagg tcctggccgc caaaccagac cgagtaccgc tccgacgatg
    12661 tgcgcaaggt tgtcacgtcg ctcaatcggt ctgtagcaaa gctcgtcgag tctgtgtcgc
    12721 tggccgtcca agatccactg atagccgagt cggaggggct cgtcgcccag gccaatcgcg
    12781 gcaacgccga gttgcaaaag ctgcagcgag ccctggcgat accggcagag caatcgcgcc
    12841 aggccgctac cgtcgacctt ccggccttca agggcgctat cccagcagcc atgattcgag
    12901 tggcccaggt ctggggagac ctcaacaccc tgcaccgtgt gtccctcggg tggataggag
    12961 ggacgcaagc cgacataatg gtccagggct accgcgatgt gctggcggcc gtacagggga
    13021 tgccaggagc caagaaaatt ggcttcgtcg ggcggcacag gaaaaagatc gctatcgggg
    13081 ttgtggccgt cgccgccagt ggagctggca cctggtggat tcttcgccga cgccgcacaa
    13141 tgatgctgca ggctcctgag atggacgccc ctgaagagga tctggaaatt ccggtgggga
    13201 ttcacttctt gccagaggga aactcaaagc tggcaaaggc ctcggagctg gtcaaccaca
    13261 tcgccagacc cgatgaactg ggcgcaccca gctttgcctt acgcgacctt cctcgcggca
    13321 tgcgagaaga aatgttcaac cttcacgcca ccagcgttaa ccctgacgca acggagcgag
    13381 cgttcgctaa gaagcgcgtt ccagttcgcg atgtgagcat cgccaagatc gacgtgtctt
    13441 cgtataagga tgaagactac tttggcaaac aagacctcgc cagtttgcca ccaatcgtga
    13501 ttgcggatgg caagctcatc gacggtggcc atcgagtggc agccgcgcag cgcaagggca
    13561 taaagacgct tagagccata gatatgacag ggctcctcga ccctgatagc actggttcta
    13621 tcgcggagct taggcgtgcc ggaaagctgg gaggcgagca gagcgccctg ccaccaggat
    13681 tcgagccatt cgatgtggac tacgacgctc ctcgcaaaac caagcctgag tgcggagagc
    13741 acctcagtgc catgcgtcga ggtgacgttg tgcaagtggg cggatcacga tgggaggtca
    13801 ctcacgccag aggcaccgat gtcctcgtca tcaagcacgg caccaagggg cgcaagctct
    13861 acaagctggt gacggtatcg ctggacccct gcgaggtcga agtgcaagag gtgcatcctg
    13921 gctccggaga cattatgcac aacgtagcac cgagtgctcg aggggccctc ggggactagc
    13981 cctcctgagc ttccagtcgt gcgacattcg ctttccaggt tgcccgcacc atgaccttgc
    14041 cagcctccgg tagcagtttg attcgagggc aaagctctgc catctcctct ttggtcttag
    14101 tagcgcgtag cgcatctata accctggtgg cctcggccgc tatctcagct gtgatctgag
    14161 gggcgtctag cattgtggtc acctcggatg cctggtgcat ctcatcggtg gtgtagaggc
    14221 cgctcagctc attcgggaat gcctcacgaa gcgccagcgc ctccgcacat ttcgccagca
    14281 tgtgcggccc tcctttggac cacatataat tcagctctcc accggccttt gtttgagcgt
    14341 agaagtccca gtaggccaga gctgggtagc cacggctgtg ccccttgcga tagaccgtga
    14401 tactgcaggc gactggcggc tcgcttccag tccagatggg aaggaagttg ccatccttgc
    14461 cgcaccattg aggcgcgtcc atgccgtcgc gctcacccgt ccgctgagcc accaggcgaa
    14521 acccgtcgat gccggtttgc atcgtggccg gcgagccctt gcgcttcacc aggtagatct
    14581 ggcccgtgaa cggatcgagg cccgtcgact tgcagcgttg gacaaaaagg gccagctcgt
    14641 cgtcggtggc atcgggacaa atcgtcctgc ggataaggtc gatctgctcg gctgtgaaag
    14701 atccctggat tggggaaacg gtggctggtt gcgttgctga agtcattgga agtcctattc
    14761 tcaccctgtg ggtaacctgt ggtctgcgtg tcaagtacaa ctagactcgc acaggcctat
    14821 gacaatcgat gttcctttcc aaaaattatc ggaacaaaaa accttgaccc gaggctcact
    14881 cccggcgaga actgatgcgt ggcaaggcca aaattggatt tagaaattag ggagcgtcga
    14941 gagacactgg gtagaagggt gaagattgag agggccacat cgggcctgtc gcagtcggag
    15001 ctggcgaccc ttctcggtgt ggcgaagatc gacgtacaga ggtttgagag cggtgccaag
    15061 gcaataccgc tagacctact gccaaggctc tgcgagtgcc tgggcgtaac ggcctcgcgc
    15121 attctagacg actaccgtgg gctgatcacg agggcagcgt gaccatcaat cccaagatgt
    15181 ggcacgaccg acacttcgtt ggcctctccg ttgatgccaa gctcctctgg tgctttctgc
    15241 tcactggcaa caatgatggt gtgccaggac tgattcgcgg aggaccagga gcttactctg
    15301 acgccatgcg atgggaccgg cccacgacgg cctctgcgct ggatgaactg atcaaagcga
    15361 ccttcattga gtacgaccaa cacgcccagc tcctgcgcgt tcctggtgcc ccaaagtact
    15421 ccccatgcac caacgccaac gtgctcaaag gatggcttag gcgctggaag gacgttccag
    15481 aatcgccgct aaaataccat cacattgaat cgcttaggca ggccgccgag gatcgcccat
    15541 ggttcgagga ggcctggtct gtgacttttg ccccaatcaa ggttccagat catacaaaac
    15601 aggtgccact tttcctcatt agccctgcca agggatctat agcaagtgaa tctgaggtat
    15661 taaacccttt agcaaaccct ttagcaaacg cttccgaaag ggtttgtgaa acaaacaata
    15721 cctataccga tactactacc gatacctata ccgatacaga tacctctacg cccagccgag
    15781 agagaccgag ggtcagagac cccatggagc ggcatgtgtt cgccagcgag ctttggcgga
    15841 tccaggaaga gttgcgttcc gagcttgcgc ccaacgtcgt gacccggccg cccactgggc
    15901 atgacctgat cccgatcatg caagctctgg agatttactc tcgcgaggag ttggaaaatt
    15961 ctctgagggt caacgcagcg acggccgcga acaagccaga gaagctggag tacttcaacg
    16021 gctacagcaa ctggcatcca aactccttgc gcagaaccgt tggccaggtg gtgccaacca
    16081 gctccggtcg acactcggtg cgaggacgga aaaaatttaa gggaggaaat gttctatgaa
    16141 aaaaaatcag agcagctcgg tcggagagag tttgaagaaa tcgatagcga aggcctgggc
    16201 ggcattcgat gatcctgaaa accagaaacg catagcggag ctgaatgctg cagcttccga
    16261 caaggacgtg agaacgcagc gtcatctgcg aattaaaaaa ctccgtcgct ggggcgttcc
    16321 aaagatagct tcggaaattg tagtagatgg accgctggat aaaacctcgg ccgtcctggc
    16381 agtgggtggc gacgaggtcg cgctgctgag agttctggct ggcaaccctg gctgtggaaa
    16441 aacaatcgca gcctgcacca ggatcgagga ccgcgagacc ggattgtttg tgagagctgc
    16501 agagctggtc gagcttggca gccactactc ggatagaaat cttctcagca gctatcgccg
    16561 ctgtggcgtg ctggtcattg acgacctagg catcgagtat cgcagcgacc agatgatctg
    16621 ccgactcgac gagcttttgg acagccgagc tggcgcagga atgcccacga tcatcaccac
    16681 gaatttcacg gccgatgagt tccgagccag gtacgaagag cgactttgga gccggattca
    16741 ccagttcggg agttacgtgg agttagacga cccagatctc cgcgtgacgc caattggcca
    16801 aggtcgaagg ccctgatggc cccgatatgc gtcagaaacg ggccaatgag cggcgagggc
    16861 gggtccggac tcacacggat accccggatc gctcagatcg catcctcgtg cgcccagcgt
    16921 gttaatccgg aatatggcta tcgggtcgtg atatgccact gaccatgacc cccgagaggc
    16981 ccccgcgact gacccagcag ctaatgttcc aggggagtct gcagctccag gctagaccgc
    17041 gccgcaattt gaaccggatt ggtggcgcaa ttttctggtg tgccgtagtc ggcggcctgt
    17101 cattctcgct gtggttttct tgataggatt ttcaatggac gattcagaaa aaatctcact
    17161 caacgcattc tcaacttcgc gtgaggcatg cgctcgacgc tccgatattc agcggctcgc
    17221 aatcaagtac tactcggccg atatgaccgt cagttggatt gactgcgtgg ccatggctcg
    17281 catagagctc gacgctgcag ccgtcgtgga gaactgatgc caaagacgca accatggaca
    17341 ccggagccac ccgtgtggga atggaacggc atatcagaac gattccgagc gcgagccaag
    17401 gagcgatatg cacagcgctc atccgccatc gagagcgatg ggcaagctcg aggaccagcg
    17461 gagcttcgcg cttactgtga gctggttgag gtgatgcacg agaagggtgt cttcaacacg
    17521 gagcacatcc gtagccacga tgttatccgc cagctccaca gagctgagaa cttcaaaagg
    17581 cacgatgcta tcggccggcg catcctggcg gggacgtggc cgaaaagaga aaagggcgag
    17641 ccgcctagaa ctatctaggt gacccgccca atcgtgtgac gagatcttgg actacagacc
    17701 gagccagggg ttgcacacag gagcgtgcca cgccttgatc gtgatgtgac agtcgagtgt
    17761 ctcgcccgcc aacaggcttg gagcgtcgaa gatgtgcagg atggcagggc tgccgggagg
    17821 taggatgaat gccgactcga agctcgtgtc ctggtagcca cgctggacca ggaagttcat
    17881 gatcttgcga gcagggttgt aggaaccgat aggcatgtcg agcccgttcg gtacggcagc
    17941 tgccgtgtca ctgttccagc cgtaagcctc aatctcaggc aatgactgat tggcaatgtt
    18001 ctgacgcttg gggatcgtct cgaaaagggt cgagatattg tccgtaggcg gcaaaaggtg
    18061 cgaggcgaag atgggcttgc actcaaagat ctgactctta gagggatccg aaccaccatt
    18121 cgagccccag gaggctgagt ggatgataag gtttttagtg gcgtgcgagt tgcggaacca
    18181 gtgaaccgcg tatcctcggg atgtttgccc agggccgggg gcatcgttga gcggcgtcac
    18241 attagcgtgt gaggggatga ccatctggcc gctgtacatg atgtaggctt cggcgtcgtc
    18301 ggcagaaacc tttgccagca tgtcgtagaa cgctgcgctg ctgaggatgc gcttggagtc
    18361 gctcacttct gcgcggttgc cgcttggacc aataatttca gtgttgctca taacgtatct
    18421 ccgtagtttg tgggtttgtt gctgctaagc gtctgccaga ttagcagccg caaaatgaga
    18481 tctattccga gagcttctct tttatttggc gaagctcttg gagaatctca aagagtaggt
    18541 tttgtgtctg gttgtcggtg gtggcgatct gttttcgatc tccatcgcta accagggctg
    18601 cgtcgtcgac atttcgtttc ggtgcgtatg acataagaat ctttccttag attataaccc
    18661 agttgccagc tccgtcactg atccaggtct tggacacctt gtcggcatca atggtgggcg
    18721 actcaataac atctgtaccg tccggaacaa cagtgatgcc atgagtagag catccaccct
    18781 taaagttctt caccgtaaag gtgtcattga ttgacccagg agcacttgca ctcggggcat
    18841 ttatgacgat gggtgcaatc gaggagtcga cgagaatgac atcgccattt ttggcgttgt
    18901 agggattgtt cgtatagtct acgtttacga taaaaggaca gcacgatccg gcgtcgtcgt
    18961 tggcgtcacc ctggctctgc caaggctgta tcggaaggtt cgtcttccaa ccatatccgt
    19021 cgttgccaat tccgagctct cgcggcacac cgtgccatcg ctcgcagaga acgtccacgt
    19081 cagcgtccga gttgccgagg ttcttcaccc tgacattgac cttgtctgcc gtgatctgga
    19141 ataggccttc ggtgcgacgc tttatctcgt ctggtatgac ggacgttccg gaaatattaa
    19201 gtccgtgcgg aagctcaacg gttgcgccag gcgcgactgc gacaaaagaa actatggcct
    19261 tgagtacatt cctcattatt tactttctga gtccatctgt gcctcgatgc cgatagtatt
    19321 catactcatc ctcgacgagc ctggactcta aaacgaaatc tggcaattga aaacccacag
    19381 ggtgatcttt gcctgggctg gtgtcattga gggcctctac gtcaatgtcc tcaagacagt
    19441 attggcgtcc ccggggtgga gctggcaggg acacccagag cgcctctgcg atattggttg
    19501 gcgacacttc aaatttggtc agggcgtcaa actcgcctcg actacccttc gcagttgggc
    19561 ctccgtagtc ctctaccacg ccggtattcc agtccataaa cttgcgaagc gcctctgtct
    19621 tggtcttata gcagagcctg tccttgggta ccttggtcac ccaccagcgg tctggcatcg
    19681 agtagagcga gtgcttggcg gccccgagcg tatcgggcgc tgggcagctc aagcgatagt
    19741 acgtcacctt ctcttcagta agatacgcaa ccctcctcac tgcgcggccc ttgcggatct
    19801 gctttttcca gaacccctcg gccgcgtcag atctatccat gtcgctctgc aaggtcacgc
    19861 ctcggcggca agcctcgacg gccgccgcat ggtagagctc ggtgccaagg ccgcgccctt
    19921 tgccgtgaac ccatgaggac acaacttcaa ggggcagggg atctccgtgt tttacaccgt
    19981 tgcgctcgtc gtatggcatc cacgcagctc gccgagcagt cacgcatccc tgcttctttc
    20041 cctcgctatc ggtggcgcac acctcaaatc ccgccgggga gccaaacttc tcaggcttgt
    20101 tggtcacctg aatatccaga ccggaaaaac cgagacccgc accctttctg cctcgccgag
    20161 ccagcagggc aagcactcca gctccagcga tcagggcgat cccgatgaca gctcctttgc
    20221 gaaccgattg cgaaaagcta ccaccggtaa cctgcccagg ctccagtgct acctgctgag
    20281 ctttgacaag gagtggttcc gggtcaatct gccggccatt ctgtaggatc tcgaagtgga
    20341 gatggttgag gcgaaagcca tccgggttgt ggccaatgat gccaaccgag tcgccggcct
    20401 tcacagcatc gccggccttg accagcagct tgctcaggtg aaaatatttg gtggagaatc
    20461 cgttgccgtg atcgatctcc acgcgaccac ccgtgccgat ttgccccgct cgagtcacga
    20521 ctcccgcgtt aaatgccaga gctggcacgt cgctgggcat gaaaaacctg cgcgagaatt
    20581 ctgggagcct caggtcgccg gcctgctgtc tcgggtacat gatatcggca ccggcgtgga
    20641 gccggccgcg ctgttcgctg aacccgaagt ggtcgcttac cttcggaatg atgccgctca
    20701 gaatcggtac tgggaatatt ggcactactc aggctttcta ttcttcaagc tctcggtgcc
    20761 gtcgaagatg ctctcatcct gcccgcgtat cgatgtgcag ccgccgcggg cgacgatgac
    20821 gtgatcaagt actggcacgc caagtatctt tccggcctcg acgaggcgac gggtcaggag
    20881 gatatcctca cgcgactgct ctgggcttcc agagggatgg ttgtgggcca ggatgacggc
    20941 cgtagaattt gcagccaggg cacccttgaa gacttcgcgg ggatgtacct cgacgctggc
    21001 caccgttccc ttgtgcgcct cctcaatgcc attgacccgg tttttgctgt caaggtaaag
    21061 cacgtagaat gactcacggt cagcattctc ggctgccttg agcatacggc atacggcctc
    21121 tggtccgtca agggatgcgc caggcttgct gtcttgggtc acacagacgc gcaccctctt
    21181 tttgccgcca ggcagggcgc acttggattc cagtgctcca agcggcgcag cgtcggcagc
    21241 tcttgaattt gtccgcagac gcttgaagta tggagccttt gctttcagct ctcggcgcgt
    21301 gtagcactca ccgtctggtc ccctgaattt attgcgcggg catcgcttcg ccatgcctgc
    21361 atcctacact acgtcggtac gatagggcca cgggctgcat tctcgccgtg gagcttggcc
    21421 tgctccacgc cctgggcgcg agccttctcc cactcctcgc actttcttgc aaagtgccca
    21481 aggtcgtttg ccgtatccac ctgatccccg ccaatgccag gaagaatctt gagggccgcg
    21541 gcaatcatgg ccaggcgctg atacggccag ccgcgcacgg tcagcggatc caggttgcca
    21601 atggcaaagc gcacgatctc catcgcatcg aggaatgccg cgtgaaattg ccgggtggca
    21661 tcgccgccag cgtccagctt ggcattggtg agctgctctt ggaggctacc cacctccgca
    21721 gtgagcgctt tgacgctgtc ctgcaggttc tgaatgtcct cgagctttac ggatacggtc
    21781 gcgtctttga tagccatggt tttcctaagg cagcaggtat ccggcccctt tgagccagac
    21841 tcttgggtta attggcgcat atttcccgac aggactgacc tcaaaatgga ggtgcttgcc
    21901 gtcatgaccg cttggattgt cgccaataag gccaagcggg tggccagcgt ccactcgctg
    21961 cccgatactc accaggcaat tttgcaggtg gaaatatcca gtgcgttcgc catggccatg
    22021 gtcaacccag actcgatggc cagttttggt gttgccggcc tcggagacta ccccgtcagc
    22081 ggccgacacc gcgcaagtgt tgtccgggta ccaccacttt atggtgcctc gcttggcaat
    22141 cgcgccaccg tcgccaacgg caaccagggg gtcgccctcc tgccacttgt agaaaaaatc
    22201 gacgccctta tgcgtgctgc gagaaggatt ctctgtgtag aagccgctgg tgattaccgg
    22261 ctggcgaccg tcagttagag ctcggagtgg gtagaacttg gccagggctg ccgcgccacc
    22321 caggatcgat cctaaggtat ttggcccagc gaacccgtcg accgtcagtt ggtggtgccg
    22381 ctgccagcac tcgaccagct ctgtgatcat ggcgtccgtg agctttccgg tgcggtactg
    22441 ctttgcgttg tattctgatg cgttcacgag tgcttctttc gtctgtgctc cgaaaccatg
    22501 tcgacggcac tcttcaccag gaagagctgc ttcatttgat cctgcgtcac aaacaggacc
    22561 gtaaacggcg agcgcttcgg tccagtattc tcgacggtta taaaatcgac gccctgctcg
    22621 accagggaaa ccgagccgcc agggaagttt tgcagcaatg gcattacgcc tattggcaat
    22681 ggctgaaatt ttctggtcgt ttcctccccc ggggccagta ttccgaagat tgggcgcggg
    22741 ccgcgcagtt gctgtggcgt catgccttag cctttgccgt gacgaacgac ttatagtcga
    22801 cgcctacgcg cttcataatc ttgaccaggt tggatcggtc gatgccgaga gccttggcgg
    22861 tgcgggtgat gtttccgccg gtagcggtca gtgctccctc aatgacttcg cgctctttct
    22921 gctcgatagc cacccagaac ggagcgtttg gattacgcct cagggccatt ggattcctcc
    22981 ggattggtag cctcttcgac ctcgggtacg gtcaatcttt cgagggcagc aaggtgtagc
    23041 caggatacga tgggagtcca aattatcgcg catgagccca cgacaaatgg cagatatgga
    23101 gaatcggccg gtattgacat gaggggcagc tccagctccc tacccagggt cgccgacagc
    23161 gcgagcccgt accaaaagcc ggtgcaggac gcgcaggaca tgaaccggtc gagccgagcc
    23221 ggatacctgg accaaatgaa cttggagatc tgagcccgcg ccaacagata gtaggccgcg
    23281 gtggtgagca gcgggtagag cagcgctgcg gtcactggtc gaccttcttg aggttcgcgt
    23341 agacctcggc cgcgctcgga tcgctggccg cttcctgggc ggtgacaatc atgcgcccag
    23401 cccagctatc gcactgaaaa tggaagggga cgaagtcgcc aggggtttcc tcctccgtgg
    23461 cggcccgctt gtcgcggata aggcaaatgc caacggcccc aaagttgtcc cgcaatccag
    23521 attcgagctt ctggatgtgt tccctaatac catcctcggc gcagccattg tcgcggagcg
    23581 tggcccggtc cctctcaacg cagtgtcgga agtaggcgtg tgccttctcc tcgtggtcga
    23641 agttgaggca gttctgacac cgtcgcaggc gggatattgt caattccgac gatcttttct
    23701 tgacgcctat gacctggttt ccgtccgcat ctacggctga gtaggcccgc ttgcgaaatt
    23761 ccttgaatag ctcctcgtcg tttgacatcg gcgaagaaca taaggcaact tgcatacgtt
    23821 gacaagtgcc tacggcagcc ctagtctgcg tgtaatatgg ccacatcgaa aaagaagccc
    23881 aaggaatttg acgaagctgt gctgcagacg ctctcggagc cgataacagt cacgctccgc
    23941 aagatggttg ccggtaaagg cagccccatc gagcttcccc caggtccagg cgaaaccatc
    24001 ggcggccgag ggtattcggc cgatatggtg cgagaactgc cctcttttat cagcaacgag
    24061 tggtcaggtg gcggcatgta cgactgcgtg gccaccggca gcaatggtga gcagatgaag
    24121 tgggagatgt attttcaccc caaccaagtg ccagaaatta cgccgccaac gcagatcaat
    24181 tcctcgcagg cgcagaacaa tcatcaacag gggccagtgc agcagcacca ccagcaatcg
    24241 ggccatggtg gctatctgtc acaggctgcg cagtcctaca ggcaaacccc caaccagtac
    24301 ccctttaacg gtcagcaggc ttcaggcatg gttccacaaa acaacccctt tggatggcag
    24361 aatggatacg agggatcgtc catgcgcttt ggatcttccc ctcaaagcag ctacacacca
    24421 ccaacgcaag atagtgcagc cgccaaggag cgcgaagagc gcctcaagct ggaggccaag
    24481 atggagcgac aggcgcagca gtcccagcac gaaaaggaga tggcggcagt caccgctgaa
    24541 atgcgccgcc tgagcgagca tgtttccaag cgaccgagcg atgacgagtc gccagctctc
    24601 aaggcagcca tggacaagat caagtccatg gaggcacaga acaccactca ggtgctcatg
    24661 caacaaatgc aggcgatgca agagaacacc aatcgcatgt ttgagcgcat ggcggccgac
    24721 aatgatcgca agattgagga gatgcgccga gctgcagagt caaacaagca ggatccgctg
    24781 attccaatgc ttatgcaagt gatgcagcag cagggcacgg cgcagcagca gtccatgcag
    24841 accttcgcgc agttcatgca ggccaaccag actcagcaac tcgagcaagc tcgccttgcg
    24901 cagtcaaacc agatgcgccc acaggaaatg ctagatctgt tccgcgctgg caacgccggc
    24961 caggaccaac tggcttccgc ctacggcaag gcctgggagc tgatggccaa tggggttgag
    25021 tcaatcctgc atgcgcaggg tccaggcgtg agcccagtac tctccatgat cggtcagggc
    25081 gtcgagggca ccctgggcat ggcccagcag tatcttgaga tgaagcaaac cgagactcag
    25141 gcaaacgccc aagctcgcac gattcaggca caggtcgagg cgcaaactcg ggtcagaacc
    25201 gcgcagatcc aggcgcaggc atccgcaggg cttcgagcgt ctgaagcgtc cctggacgat
    25261 tcgatagaag atcaagagga cgaaaccgac gaaaccgacg aggaggagat cgaggtaatc
    25321 gaggcgtcga gcaaagagca gatcgccgca aaagaggtcc aggtgtttgg cgcggcacac
    25381 gaaaacgtga tgaggctccg aaagggagtg gcgtcaggtg ccatcgactc gtcaaagatg
    25441 gccgcgattc ttctccaggc catagatcac tttggacaag cggcagccaa tggaacacca
    25501 ctcccaccga tctttgcgct gttccaagag gagcgcttcg ccgatctgat cgacgccctg
    25561 gttcctagcg ccaaggtgca gtaccgcgag gaggccaccc aagccctcta tgcaggcgtc
    25621 gagaatctga agaggcaggc cggcatggcc tccgcggagc cccagccggt agcgtgatga
    25681 taccggttgt caacaagtgc agtgcagata tcgtcagtgt tggcgatacc tggacgtgca
    25741 tcgagataag cgacgacgcc actgacgccg gcgacgttga gctcgtacac gtcgacttca
    25801 atgccaatgt ggcgaccgta agatacaaag gcaatattca ccgtggcccg atgatgatca
    25861 ggagcgacca ccctagctac atgggcagag aggttgccgt gctcttcgtt tagatcttcc
    25921 gcgatccgtc gagtcttctc ggtactgggc agctcatctc cacgtagcgg tcacccctgg
    25981 tggccctgcc cttccttagc tgttttttcc agaatccctc cgagagtcta tttcttccga
    26041 agtccaaaga tctgaggcgc atcccagacg cgcatgcctc ttcggcgact agcctgtaga
    26101 gctcagatcc tatgcccttg cgcagatatc cctcgaccag gttcgctccc tgtatatcgt
    26161 agctgtaggc ggccttgtta gcgcccactc tcccgatgac cttccggccg tcgtaggccg
    26221 tcgcgacgat cccaacctcc tgcttccagc cgctaggatg ctttcggtat tttcgcttaa
    26281 ccttgtatcg aattggcatt gcttgatccc tttacgatga gttgccagat cgcccagcca
    26341 gccatcatac ctagcgcatc gccagttgca ttggccagcg tgtcttcgga cgagtacgga
    26401 aaggctcgag ggattgcacg cttgaggggg gtctcaataa gctcccaccc gatggcgaaa
    26461 attgcagcct gccaccagtt caaacgcatg acgccgagcc ctatgccggt tgcaaagtgc
    26521 gcgacggtgt agcggtcgaa catgtggtag ttgacctcgc ccttgcgagc tgggttgccg
    26581 agttgcttgg gggttggcca accttcgcag tcagccgcag ccttccattt cgaccctacg
    26641 cgcaacgcag cttgcgttgc accgcgatct ctgccggcat gtagtaagct cactacggag
    26701 tttgcctggg cggtggctgc cgaccactgt cttttgcacc tggagcttgt tctggccctg
    26761 gcactcagtg agtctcgctc cattgtggca attttaattg cctgcttgcg gggtatggat
    26821 acgaagtctg ccaaaaggag acctagtcct ttttgaagta ctgcagcaca gcgagcagtg
    26881 cggtcgtccc cgctgccatg ccagcgatgg caccccagag catgcgcgac cgtcgctctc
    26941 taagagccag agctttgtcc tcggcctcca gcttggctgc aacaagcctt gcaacagcct
    27001 cccgctggtt cggatcaagt ggctttccgt ccgactctgt cgcgatctcc tcgccgagcc
    27061 cgcacgggcc gccgactgcg catccagagc agcacgcacc caggtcgccg gcagtcgata
    27121 gctttccgga gatccatccg cgatgcaccg ggaggtaacc gtagaccttg gccagatcga
    27181 agtcagttgg cctcgcgtca aagtgagagc cacccatgag ctggccgtag gcgttgcgaa
    27241 ctttcacgcc aggatcttgc tgagctggtc tgcgatccag gccgaggtgg cccatgaggt
    27301 tgacggtgcc accagatccg atggtgagcg tggttgttcc cggtgcgaca ccgacgcggc
    27361 tagctctgat ggacatgccc aaaggctacc acgaggctac tagcggcgta tagcgacgaa
    27421 gagggccgca gccccgagca gtagaccgat gccgccgact gccatggagc ctatgatcat
    27481 gggcttgacc ttttcctgag cgccggccgc cgccttctgt tccacaaggg tctcgaagtc
    27541 gtccaagaac ttagcggcct tctccttgcc catgacgagg ccaacagttc ctatgaagtc
    27601 ctcccgaaat ccgcctagct gcgctccgtc gaggtgaatc gtcgcgagct ttatgtagta
    27661 gtcaggatcc tctgccaggt gatccatggc gatctctctg gcgagacgag cattgcctgt
    27721 atgctcaagc tccaccctgg ttccgactgc tagttgcccc gggtcgaaat cccttggcct
    27781 cattccagat gcgcggccac ccggtatcga ttcccggtac ccgagatcct gaatgcgctg
    27841 gctaccgtag ggcacctgga cgtaccgctc tgcacatatg aactgctctt gcccgatctg
    27901 ggtgccgatg atcatgctgc tccctggcga aacccgcgct tttgagcttt gtcggcgtac
    27961 cagacgatga ggggcacgcc aaccatgaga gctatcaccg gaaagacctc ggcgagaaag
    28021 ctcttctggt tttttcccga agtgtcggcc acgaagatcg ttgtcagtgg tgacacggcc
    28081 gatagctgcc gagtgcttcg cctcatctac cacccctgag cataaacatg ccgatcagtc
    28141 cgactcctgc gactgccgag cctatcaggg cgtgctttac cttgactggc ccaacgatgg
    28201 ttttgtcgag gaagctgtcc tcctcttctt cgggcacgaa cacaacattt tgcacggcag
    28261 gagaaagcga cggtggcagc ggttggggcg ttggaattat gaacgaaggg ggaggtgacg
    28321 gtgccacaat atctctcgat ggaaccagcg gcttggtgct agacgttggt gagatctgga
    28381 cagatctcac catgaacctt tttctgtcca gcgtgccagg ggacaccggt ggcggcagag
    28441 gagctggccg gcagaatctc tcgatatcgt cccaggtctc acccttcacg cacccatgcg
    28501 agtccgtggg ctctgcgctg gttgccgcga cagttccagt ggctatggca tctccgcgga
    28561 gctgggctgc cagcccggta gcggagggag ataggccgcc gcgcctagtt tgagttgcgg
    28621 agtttccgag aaatagcatt aggcctgcac cgcttccgat ttaagttttt caaattctcg
    28681 cattgtcatg tagccggcca tcccgatgcc aatcgccgcg ccaacgatgc caccaagact
    28741 gctggaaact gcggccttgg cggaacgtcc aatcgcaaaa ccagctaccc cggcaccagc
    28801 cccactcgcg ccaagcacga tggcccacgt cataagcgga acgcctagga tggccctcga
    28861 cggtccgaag ccaagcgtcg tggtcatgtc gggtgtaatt attcccaggc tgcgctgagc
    28921 ctcctgggag atgaagccaa gcgtcgtggt catgtcgggt gtaattattc cgagcctgtg
    28981 gttgtttctg ctgtgcatgg tgcctcctat gcgacgaagt ctagcctctt ggcgtgcgga
    29041 gcttcaatgc cgtagcggct cccaggtaga gtggtgtcaa ctgcaatgta cttcgacggc
    29101 cgcatctttg gttggccgat cattgcgtaa atgtgggtgt agtcctcgtt ttttcctttg
    29161 cgaggagagg taacgcgata tttgcagtgc attccgttga ggagtgccga cgtgcaggcc
    29221 aggatcgcgt ggtcgtcgca gtcgcccccg ccaaactcca ctgtgcggta tgcagtctgg
    29281 aacaggtcga cgccttccac cgggccgcca gcccagagtg cgtgtgggcc gatgtcaccc
    29341 gtgtaccgga cgtttttctt gatccagttg tagatcgact tcgcttcgca gttgctgtcg
    29401 cgctccttgc acttgctggt catctgcagt gcgatcttgc gcatgcgagg atctttgacg
    29461 ctcttggcgg tgagatcttg caggatggca acgcgatctt taataggtag gtcgtcttgg
    29521 cgatgatgcg tgagcttgta cccatcttcg gtctcgagcg agtctgcgat ccggccgagc
    29581 ggcgtggttc gcttcttttt ggtgtagtag taggcccctg cgcccaccgc gcctatgcca
    29641 agtcccaaca ggccgaggta taggagtttc ctgttatcca ttgcgacacg gtatcacggg
    29701 cctagaatcg ttgacagtgc atgccgaacc agggtcaact tgagaaatgc ttattgctgc
    29761 cgaaaactct agtggtgtgc aacttgtcgg atctggcttt ggcgacttcg ccggcaccgc
    29821 atccgttcag cagctacagg aggcactgct ctccctttct atggccgccg gatggaccgc
    29881 cgccaaccca ggaccaatca ctggcgacgt gagtctgcaa accatatcgg ccattgccgc
    29941 cgtggttgcc aagctggggg gcaaaatttc gtccagcgtg aagacggccg tccagctcgc
    30001 agcggtaggt gccgcatttg acagcacact gcgaggtgcc gcccaagtgg cgattgccgc
    30061 ccatgcaaac gagctcagag gtggcgttct ggctttaacc gccatttaca caaaatcttc
    30121 cgctcccccg atacccccag ctcctcccgc caagccacta ctcatcacac aatttatgat
    30181 gaagtcgacc acgccatccg cggcgcagac ggcaccgctc ccacctggat ccgttcaggc
    30241 gcagacgaaa gccggcatat accgagttgc cgtgccggca tcgcaggccg ttggcctggg
    30301 cgggccaagc gttagtcttg gggaggcatt caaggagcta ccggctcgac agtcatttac
    30361 ccctggcgtg atcctggtat ccgagggtga tctcaaaaaa aagaccggcg aagcaaagcc
    30421 gttttggaaa aagccgatgt actgggcaat cgtcggaggg gtcgtggttg tcggtggcgg
    30481 cgcatacacg atgatacgaa ggcgataacg tgatcctcga tagatctaag agtccattcg
    30541 tgccggtggt catgccgagc aattttactg aagaggatag tgtctatccg ctgtttggtg
    30601 ccgacttcgg cgagaccgag gaagaggcca tactccgtaa gcgcgacgag cttttccaga
    30661 agctacgcgc agaggccgac gcagaggccg gggccgagga ggccaagcag gtcgccagca
    30721 ggaagatcct gatcttcggg ctagcaggag ttgccatcgg atcgttggtc acaggtggaa
    30781 tgactgcctt gtcactgagg gcttactcca ggtctggttc ggtcatggga ccggcgatat
    30841 atgccggcat cggatcggcc gccatgggga ccgccatgat cctgctgcta tcgaaaattg
    30901 gggcgggcaa gaccgtcgac cctcgcgctg ctgcgctggc tgtcggtgcc aggatggcgg
    30961 cctagatggc tcgaaagctc ccaaagaagt ttctggcatg tggtgctctg tatcagaact
    31021 accgagccaa caaagacgac aacgtctatg ccgcctgccg catagtaaag aaggccaatc
    31081 gcagcaagtg caaatggtct gctaagcctg gtgtcatcag gagctgcaaa ctgcgctact
    31141 gaggacacca tgagcaagcg ctgcgagatt cgacagatcg acatgggcgg caaaaacgag
    31201 ttcttctttg aactggtctg caatggaaag cgccaggcgg caatccatgc agccaagaag
    31261 aagattgccg gaaaggttcg cttcgttgtt cgcggagtga atgtgctcga gcagtttcgc
    31321 cgcaaaggac ttggaacaaa gctctacgaa atggccgccc aggaggcgtg tcgccgccgc
    31381 gccccgcttg cctcggatga gcgcattggc gatatgtctc gtgagttttg ggctaagcag
    31441 attcgcaaag gcagggcgac ggtgctgtca aaaaagggtg gcagcgagaa gggagagcgg
    31501 gcaccggtgt actcgctcac ctgtccagcc ccaccatcac ttcgtggtag ggtgaaaaaa
    31561 tgaaagacat attcactggc ggcaagggca cgattgccgg cattcactgg gccgtatggc
    31621 ttgcactgcc agtcgtatct gctgcgggcg gtgccatcac ctccgggcct ggtagaagag
    31681 gatactctgc actcggagct gccggcggat cgctgatagc tctgaccgca ttcgccatac
    31741 ttgagatgca gccgtctggg ccgcgtccag atccggaaga tctgaagagt ctgctccgct
    31801 aactgtttct gtcgagagca gctcgcactg cgagcacatc aaattttacg tggccgccga
    31861 ttcttatcga aggaacaatg ccgctgtgaa cgtatcgcct tagagtcgac ctaccgatac
    31921 caaggtagtc gctaatctct ctcgctgaca ttagcggcac gacggatcca tctccgtttg
    31981 tcgcgaccat gcacgcacgc ttctcttcga tctttacctt gcgcggcaag ggctcatgag
    32041 atcgcgattc ttcttttttg ttcttagtca tccccagctc tattgtgatg gcctgcccaa
    32101 actgtgtcaa ttgtgctcgg cagtgctcga tagtgctcgc tcgcgctcga ttgtaaccca
    32161 ctgaaatcac cgggtaatcc gttgaccggg taaagcgttg gcggtagcgt agtgagcatg
    32221 aagcatgtgc atgtgatgaa catcaggcca gacgtgatta cacgcaccgg tgttatccag
    32281 ccatacgcta tccgcgagat cccaccactc gcggtcgccc gcaattccgt gcaggcattc
    32341 gagcaagtag cgcgaccatc cggcccatac gttcagtctg agccccagcc agaagaagta
    32401 gcagctcttg ccaagcgcct ggctactgcg atggcgcagg gggttaggcc accggcgggc
    32461 acatcgccaa aactaattca ggctgcaagg tacatagccg agggcaagcc atttgcgatc
    32521 aagtcggccg ggcgcaccgc ccaggtcaag gcatccccag gatacgctgg ccgcaaaaac
    32581 accgtcgtgc ctgccgttcg cggccgtgca aacaccgtcg tgcctgccgt tcgcggccgt
    32641 gcaaacaccg tggtagctgc cgttcgcggt ggtcgctcca atactgtcgt gcgctccgtt
    32701 cgcggcaaaa ggtaggaaga aaatgccaag cgtatacaca atagagggcg cgtcccctcc
    32761 tcgccgacgc cgcaagacca ctagcaagca gtccgctcac cagaaaaggt ttaaggcagc
    32821 agccaagcga tgcaagggtc agtccctaac caaatttcgg gcgtgcatgc gccgcgagct
    32881 aaaaaaataa ctccgctgtg cggtacgaag gaaagcaaaa tatgaaacac aacaatgaca
    32941 acctcggcct cggtggagtt gacgcttttg gcctcgacga gatggggcaa cccgttggac
    33001 ttaaccctgt atggggtgca gtggttggca ctgggctcgg caccattggt gctatcgcta
    33061 cgcgccaatt ctctcccaag ctggccaagc acagtgagct tgttggcttt ggcctcgctt
    33121 ccgtcgcctc tggtgcaatg atcgctttcg agggcactcg agcggctggc tggactggcc
    33181 tcgcatcggc attcctcaac aacggactgc gccatatgga aaaaatgttc gctgagaaat
    33241 caatcgaggg atccgtcgtc cccaaccctc ttcagggcgt cgtgattgat ccaaccgagg
    33301 ccctgggcct ggtagagatc aacccaacat acgcactcgg agaaggcaac gacatgccga
    33361 ctctggttgg agcgaacctc gcctctgcca gcgatcacgt tcaactcgtc ggtggaccat
    33421 cactagctca gaccgctggc cactggggcg caacggtcat gggctaacag cccctgctct
    33481 tttattaacc ctgggattca atcccaccct gacacgttgg caagcagggt caccaatcaa
    33541 ggccttcaaa ggtaaagaaa atgggaaaag taggaattgc accgggtaca actaccctca
    33601 cactgccagg cggccaaacc atcgctctgt ccgactggat cgacgacaag cactggacca
    33661 tggtcgagct tgagaacggt gacaacgata cgctcgatgc gttcgcttcg ggccgctcgc
    33721 agcagatcac tggtggcact cgtgccaaca cgctcgttga caccaacgtc cctcgcaacg
    33781 gctccatcgg ccttccgaag gactgggagt tcatggtcta cggctgggct atggaggtca
    33841 tccgtgttac ccgcccaacc ggccaggaga catcccctcg gctcacctcg ttctcggacc
    33901 cagcttcgct gcgtaccctt ttcgagttgg atcgtcgcct gttctgcaag tacatctaca
    33961 acggaaagat gtacaccgag ggcatgttcc gcgactaccc acagggtcac ggtatcagcc
    34021 tcttcacgac ggccagctcc accgagcttg cccagaacgg catccccagc cctcgcgacc
    34081 gagtagccct ggtgcttccc gttcacgagc gcgaaaacct cggatactcc ttcgagatct
    34141 cccccgtcat cgcactggca attgcacagc cagcatctga cgatggcgca gtgctgaact
    34201 tcctcgatct ccggatcacg aagaacggcc ttatcaagcg caccgtcgtc taacttagaa
    34261 gcggtcgtca ttacacgcga cccctcatca ccggggggtc gcgtttttgt ttaggcctcg
    34321 gtatattccg ggcaaggaaa caccatggga cgcaattcaa ttattcctca ctctttcgcc
    34381 gatcaggtca tcacgtttcg gattccatat tcgatgcctg gagagctggt ggttgagcca
    34441 aatgcctccg gtgtattttt ccccgaggca acattcctaa cgaacgtcga taagccattt
    34501 gagattgatc gaatgtacgt gcggctaacg gcactcgacg agaacaagga cgtgctcgac
    34561 gttcagccag gcacgcttgg tcgacgagtt cgcctgtcca tcggcgacac cagcaagaat
    34621 gaaaagctga ccaagtcggc gcacatcgtt gacaccttga tcagctccga agccggcgcg
    34681 gctggctcct gggagtggta cgtgccatac accatggtgc gctccgaggg cttccagatc
    34741 ggcatcgaca ccctggcaat gccagcgggc actgactttg ttcgcgtcga gatagctttc
    34801 cagggctcgc tgatcgtcat ccaacctgcc agcgagactc gatagaggac aggtgcagga
    34861 ctccacattt cagaccttct cggttcctgg ggcgtctacg gctgcgacga ttcctctgac
    34921 gattgccaga ggtccgtccg actctgcagt cagggtggtg gtcaagaacg tgggagctgt
    34981 tccaatcttt ctggccggcg catcggcaga tgccgttagc cccgaaggtc cgtcgtctaa
    35041 tacttttcaa ctcgacccgg ccctgagcga ggtattcgtg ctggccgcca ggcagctcat
    35101 gtatgcagtt ggctcggtgc caggcgggag agtatctgtg catgtctcag gggaatttcg
    35161 tcgcctgtaa ggtcaagtcg tggcagcact aaatcaaaca atactcacgc acgaaatagc
    35221 gacgagaaat gggctcccct atttcgaggt tgttatcaga gggtggggta gtggcccaga
    35281 caatggcata ggcaggtttt ttagacttcc ggatgttccc ctggccggca tagcaatctc
    35341 tccagagagc acggttggcg agtgctacgt gctcgaggac ttcagggtct ataccggtgc
    35401 cttgtcgacc tatgatccaa tcgtgttcga ctccaagatc gaccttatcg gatcgaacga
    35461 caatccggtc cctccggata ccaccggatc tggcagtggc caaactgccg gtggagccat
    35521 ggctacgcac cagtcacagg tctccgtgca tgctccgctg ataaagccta tcagcagcgg
    35581 tacggcaatc gtcgccagga accaatcgcg ctacaacgag cactacttcg acgagagcgg
    35641 taacgaccag gacttccctt caaccagaac cagggagccg gtattggttc tgcgctgcta
    35701 cattcgcagc cctgggcccc tgtcctatcc ttcgagccga gcaccgcttc tggtcaacgt
    35761 gcgccaggct gcagttgtct tggataagga taataatccc gtacagtacg gactgaccga
    35821 caccttcgtt gcctatgcaa taccagtcgg aggccgcaag agattccaca tctcgctgtg
    35881 gtcgctgcca gatcaattca gcggtgccga cagcggggtt atcaacgtcc gagcctcggt
    35941 gctacgcggt ggagagacaa acagagaaaa cctttttaac accgattggg ctggaaactt
    36001 tttcgaggaa gaagtcatcc ccagcacggc actcactcct ggtggagcga cgaacaaagt
    36061 gcgctatgag gagctgcaca acagcgctga gtggctcctg gtattcgtcg agcagaccac
    36121 cctggttgcc aatgtttgcg acgtatcggt cgtcatgcga gcagaggact aatgaggtac
    36181 gacgtgtttc ccctggtcag cgatctgaag ggccgctttg ccctgggcga gtttacgcct
    36241 cctaagttca ttcccgatcc gcgcaagagt gatatctcgg ctcgcatatt tattgagata
    36301 tggatcgcct gggccgccga ccgcgagcag aagatcaggg aatgggccac caccatcgat
    36361 aacctgattg cgaccaagtc ggcaacgtgc gccgctatca gagaatacaa cgatgctgcc
    36421 atcgctctct acgtgagaga ggtggagatg ggggagcgct tcatactcgg cggcattagc
    36481 cgaagcgacc ttccgcctcc attcttcccg ctggtgtttg cctcgagcac gcgcaccaag
    36541 agcatcaact tcaaacttct tgactggccg actgggcgac taaccattga gtacgacctg
    36601 ccatgcccgc cagtagatcc attcgggcct aacccagatc ctcgcctggt ctttcctgac
    36661 ttctccaagc tcagggtgtt cggcgacccc agggacgttg ccagtgttaa gaccccttca
    36721 ggactcgggg cactacaggc cgtagggtgg gcggcagttg tcctcggcgc ggccctggga
    36781 gccactgcgg tcctatccat aagggaagcg ttcaggggcg tgaacaacac cgagctggcc
    36841 gagatcaacg gggaaatagt agataagaac tttgacgcag acttcaacag ggcaaatgaa
    36901 aacagggatt gcgtcgacga ttcactcagg aagctcggcc gcgaactaac cgtttcagag
    36961 ctcctcagca ttcgcaccga atgtgcgaga atcgcagatg gtcttttccc gcgggctgag
    37021 acaattgcgg agcccaagag cttcggattc gccctactac tcggtggagt ggggatcgcc
    37081 gccattgtcg gctccattgc gtattttagg cgcgagtaac atagattatg gcgtttgcgc
    37141 atacctaagg gatcgggtta gagtttggca tgagctttgg acagggaaat ggcgggggtt
    37201 ccggatttgg caattctgcg ccaggatgct gttcgcgcaa gatcggtgga aagccaggcg
    37261 gacgaggtct ggcaaaacca gtagctccac gcccagctcg accaggatta gtaaaaccat
    37321 cacggccagc tccagggttt ggtcgaggaa ataaccgcgg cggcggattc aaacgctagg
    37381 agatgagtca tggcaaagag tagaaaaaaa gcaaacaccc cccaggccaa acgattcggc
    37441 gtcattgtca aggccgccaa cgtcgtctgc caccgtgaca ccaacagtgt caacgcctac
    37501 aaaaagtgca tgagttctgc catgaaggca aagctcccca ctggcggcaa gaaaaagaac
    37561 ccacttctca agtgcaaggg cacctactcg agcggcccaa acaagggaaa agtcaagcct
    37621 ggctaccgtc gcggcgcggc tggacgttgc cccgtcaaga aaaagaagta gtccgcagct
    37681 ctctcaaccc actgatggca ttcgaccttg atcgtcggat gccattcacc tatcttcgac
    37741 ccaggtaacc atgtcactaa acaagctcta tagcatcgta tcctttgtag acgcaccgag
    37801 tggggtcaca tctctcccgc acggcctcaa ccaaggtggc actcctgtca ctcctgacga
    37861 gttgaaggtg tcgatggcac tcgaggaggg ttccgtcacc gcagacgaga ccaacatcat
    37921 cttcaacaac gataccgagg gaacggtctc gatcactgtg ctggctgagc gctggcatac
    37981 catcgagcga gagctgggcg tgcaggatcc cgcagtcaac ctgtcgatcc agccctgggt
    38041 aacctacagc gcttaagctc tatcggcgta gcgccatata cccgccgcca gcgagcacca
    38101 gcacgcccag gccgatacca ccgagcacca tgggcctctt gaaccatggt ggctcgttgc
    38161 cggtgtcctt tttgaaagtc accaggtcga cgaggcgggc tcccttaggc agcgatgcct
    38221 gggtgccaac ttcagtgaac gaggccgctg cagcgaagcc agccagccca gatggcactg
    38281 caacgcgata gccgccgcgc ttggcgtcaa aggctgcgat ggttccggca ggaagagcgg
    38341 gcggcgcggg cggcgcgggc ggcctgaaca tcatctgccg ttccagtacc agcgtaggaa
    38401 gcggaaacgt cttcaccggt ggagctggaa tggatggtcc gccaggcggc acgaggatca
    38461 tgcaggcggc cgaggttcct gcggctatgc cagccgcagc ctggcttacc cctgtcggta
    38521 ttgccgcagc cccagcagtt gcggccatga acttaggatc tcttgccgtg gcacacgcct
    38581 tgtcgacgac ccaactagca ccgtcttgga ttgcgccacc ggcatcggat gccttgtcca
    38641 caacccagtc aatggcatcg tcgacaaagc cgggccatcc gaggtcgtca tcaccgtaaa
    38701 gttggacatt tagcataccc agtagcttct caccggggat actctgagtc aatcgggccg
    38761 gctaaagagg gtatgatcgc aggaaccaga tgtccgatga aatactgcaa agaatatcag
    38821 gcgatgtctc cgagcttcag gacgatatgg gccaggtcaa ggaaaggctc gttgcccatg
    38881 acacacgctt cggaaacgga atccatgtca tgggcgagat gaaaggccgt attgaaaaag
    38941 tcgaaggcaa gatcgagccg aaagcgcccg actggctaaa gatgatgctc gccggattgt
    39001 cggtggttgg catcctcatg ggtgcccagc tctggctgac ggatcgtttc aacgacaggc
    39061 caacccaggc cgaggtcgag aagctcaccg cccccataaa ggcggcccag aaggagactg
    39121 ccaaggacgt tggcaacatc gagaaatctc agtcggcgca gcagacctct atcaagaata
    39181 tagagaccac ccaggccact cagggtggca agatcgataa gatattggaa aggctcccgg
    39241 ctcggcgcgg ggagcgagag tagctacttc ttcttcgctc tgcgcaggca tcgcttgccg
    39301 ttcctcgacc actccaggca atccctcttc tcgccggccc tgctggacgt tttctgagct
    39361 tgcctcctca gcttgggacg cacagctccg ctcacctttt tcgcacactt cattgaagtc
    39421 tcgccgctgg tgacgcaacc gcagattttc ctcgacttct tcgtggcgtc cgcgtggtct
    39481 ctgaccggga tcataattgg gtccgctccg aactttgtgc aaatgcgcag ataggcaccg
    39541 gcgggtacac ccatgactgg cgacgttgtt ttcccaacga actgcagcga cgtgtcgcaa
    39601 tccttgatct tgattttcct acccttctcc gtcagtgagc acttctcagt tgtcgtgatt
    39661 gacttggggc caggcacaac ctcgccggtg atgtagtcag gcgctatctt gcgtcccttt
    39721 ccgccgcctg gagttttcat ggcaaccgga cttgcgttcc agttcgggtc gccaatcttg
    39781 gccttgagga ttgctccggt agtcttttcc ctggttggca cacagggttt actctttgct
    39841 tttgccatcg ggtgccttta gtgggtggtg taattgatgc gaagctctgg gttgagccag
    39901 catgctctgc aaccaattac gttgttaggg gccagggcat tcttgcagct cggcgaggat
    39961 attccgatag cgtaggtctg gcacgcccag tcataccgcg tgtcctggct tcgcccgcag
    40021 tcttctggct ccgtggcgtc tgcgaatgcg tacagcgatg tggttccggc cgttgggaat
    40081 tgatatgggc cgtcccacgg gtagtcttcg gggccaggag ctgcgtcgtt cgtgtggtag
    40141 gcggacggcc gaattgaaaa gttgtcgttt ggaatcccaa cgcgctccat catgtgcgcc
    40201 cagtgcccag gaacccactt tccattctta gtggtaccca tccacccttg agctgcccac
    40261 gtcctggttg gtgcccaaaa gaaaatctct gggagcctgg cggcaacgtg cagccacgcc
    40321 tctgcgtagc gtggcgagaa gaagtcgcca gcgctatgga cgcgcacggg atagatctgt
    40381 tttccagtgc gaggatcgat ctgctcttca tgtccccatg agagccctga aattgcccgc
    40441 accatggttc ttatccactc ctcggtgccc tcggtggttt taagcatctc cctggtccac
    40501 cagtagcaaa ccatctctcg caactgaggt ccgagggttg cgtactgacc accctcggcg
    40561 tagcagatct gacagatcgt atcggtcact cgcaccggca ttcccaccgg tcctactcgg
    40621 tcgaccggct gcctgccaag gtctccgtcg aaggcaaagg gatttacgac tgcgagtttc
    40681 ttcttctggg cacttgaagg cgtgcctgtg tataggccct cggcgcttcc gtatccggtg
    40741 ggagcctgga cgttgatctt ctctctaacc tccagcggca caatcgtctg accagcggtt
    40801 gctcctgggc aactccctcc taccagaggt gaccctgccg ggagatccca gtttgggcac
    40861 gccatcttgg agttttcggt gaagaacgat atgtattctt cgtcgcgtgt tccggaggtc
    40921 cacagaatgt cgtgctgcag gattggcggg ttcagcttga agtagagccc ggccgctccc
    40981 tcgccgccct ttcctgcagt tctcccgtca acaacggtca cctcgcggcc gttcaataga
    41041 tcttcgattg ttctgcgcag ccaaaccgag cgggtgaggc cgttgtgggt ttgctcgagg
    41101 tctgggtggc actgcctggg cccttccagg gttacacccg ttgggcactg catcacggca
    41161 ttgcgatctt ccggatcgtc gtctacatgc tgctcgatgt ggatcgttat cgcgctcatg
    41221 cccctgagct tctcacatgg aggctataag caaaagtcgc tactgtttac ggtagcccgc
    41281 tgggcagcgc tttttgttgg aaaacaggta gatcttgtgc gccttggtgc caacgcggca
    41341 gcgcttggtc tgcgcgttcc acgcaccata ggggcagccc atgagcactc gcgtcacgcc
    41401 gccgctctcg cgagtgccag tcttggcggt ccagcgaaac gagcccttgt cgaagctctt
    41461 gcgggggttt tcaaacttgg cgcagacgat gccaccgtcc tcgtagtagg ccatctattt
    41521 ggtcttcccg aagccctgag ctacggctat cacgggcacc acgacgggga acatgccacc
    41581 catgagcccc caccacagag cccagccaac actctggttg cgcttgtatc cgtggtagac
    41641 gccagccgcc atgctcgcgg tgccaaacac cttccaggcg agcgagaagt ttctgcgagc
    41701 tggagagcca ggaccaaagc cggtgagcat gcccagggca gcgttgagca gggccacatc
    41761 gccaccgttg gcgacaaacg aagctgcgat cacgtccctc gacgagggag cctgcggccg
    41821 tagcaccgat gccagctcct ctgcggtcag tgacttgctg agtgccaggt ctcgcccagc
    41881 gtcctcaagc tcaccgccaa ggtggtcacc gaggtagttt ggatccagct ctgggatcat
    41941 gtagtcgacg gcaccgagag actgaatttg catccggaga ttctatcgct ttgtcatgcg
    42001 gccgacaata acgccgagca ccaagccagc gccaccagag gctgcggcga ggatgatgtg
    42061 cttgcggcgc atgccgtcat ttggggtatt gccgctgcgc cttgcgaccg ttgccctagc
    42121 ttcggctagc tcgatggcgc ttccgccggc cgcgatgaac gatgccgcaa ccacatcctg
    42181 gtccgcagcc gatagctttt ccagggtctg ggcaagaaaa ccagcattaa ggactctgac
    42241 cgcgagactt ttgccgagcg caatcagttc tgcctgggtt tccccgaagc cttggaggga
    42301 gctgccaacg agtgttactt gcatagccct gatactactc agctcagacc tggcagtcta
    42361 gcccgttggc agcggccgaa aacaggctcc tgatgcgctc ctcggaggcg tccccgtcga
    42421 tgaagccagc aaacccccgg cggccgtacc tggtgataat ggtgctcggc gtcgactgca
    42481 cgcgaaaggt gtccgcagct cgaggaaatt gggacacgtc cgcaataata atgggcaggc
    42541 agcccgcata ctcctgcgcg atcttcgaga attttggcat gaactcgtcg caggctgggc
    42601 acccgtcctg agtccaaacc aagatggcaa ccggcatatc ccagatcttc atgcgctatc
    42661 cccgtatcgc aaaaacagca acgcaggcaa tcctacaagg cccataatcg tcgtgccaat
    42721 ccggccagcg agcagcggcg cagtctcgca cacgtcggca tcgagcaccc cttcgggcat
    42781 tggagaggtg agtgcgcggc gaccaatcgg cttcatgcga gc
//
LOCUS       DBNHOH010000745         1220 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1648270, whole genome shotgun sequence.
ACCESSION   DBNHOH010000745 DBNHOH010000000
VERSION     DBNHOH010000745.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1648270"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="AC16V7_13360"
     CDS             complement(<1..528)
                     /locus_tag="AC16V7_13360"
                     /inference="COORDINATES: protein motif:HMM:NF013209.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR6258156.1"
                     /translation="MTSTRKLILDYLQIKPFATAAELSHALKMTTANVRHHVNVLMEE
                     GAVEVIAQQPPQGRGRPAHLYNLTQQAHRHNLDGLASAMLKTYLSNKAAEYQSASLKQ
                     IAKNMASTANLKPANSLPQRLFQAVHRLNEIGYQARWEAHSEAPHLILGHCPYAAILP
                     EHPELCQLDSYLIEEL"
     gene            623..841
                     /gene="iscX"
                     /locus_tag="AC16V7_13365"
     CDS             623..841
                     /gene="iscX"
                     /locus_tag="AC16V7_13365"
                     /inference="COORDINATES: protein motif:HMM:NF016284.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HIR6258157.1"
                     /translation="MPLDLTLTWEDTYAIALLLIERHPEMRVEDISLGMIYRWTTSLP
                     EFSDDPELANDAILTAIYQAWYEEVNPL"
     gene            838..>1220
                     /locus_tag="AC16V7_13370"
     CDS             838..>1220
                     /locus_tag="AC16V7_13370"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HIR6258158.1"
                     /translation="MSEELNTQGYDIPPELQGQVAITTLDKIYNWGRRSSIWPMMFGL
                     ACCAIEMICTAASRYDLARFGMEVMRPSPRQADMMIVSGTVTKKMITTIVRLYNQMPE
                     PKYVVAMGACASGGGPFKEGYTVVSG"
BASE COUNT          274 a          292 c          342 g          312 t
ORIGIN      
        1 taattcttcg atgaggtaag agtccagctg gcaaagctcc gggtgttctg gcaagatagc
       61 ggcatacggg caatgtccca aaatgagatg cggggcctcg ctatgcgcct cccagcgtgc
      121 ttggtagcct atttcattca agcggtgcac agcttggaac agccgttggg gcaggctatt
      181 cgcaggctta agatttgcag tagatgccat gtttttagcg atctgcttga gcgaggcaga
      241 ttgatattca gcagccttat tggaaagata ggtttttagc atggcgctgg ccaagccgtc
      301 taggttatgc cgatgcgcct gttgggtaag gttgtaaaga tgagccgggc gtccacgacc
      361 ctgaggcggc tgctgggcta tgacttcgac tgcaccttcc tccatcagca cgttcacatg
      421 atgacgcaca ttggcagtgg tcatcttcag cgcatggctc agctcagccg cagtggcgaa
      481 gggttttatc tgcagataat ctaagatcag cttccgggtg ctggtcatca atgagattat
      541 agctgtccaa gtgatttttg taaacatata ttttctttaa tctgggttga cggatctgcg
      601 ttctacgata taattatgcg gaatgccatt agatctcacg ctcacctggg aagatactta
      661 tgcgatcgcc ttgctgttga tcgagcgcca tccagagatg cgtgtagagg acatttcgct
      721 aggtatgatc tatcgctgga cgactagttt gcctgaattt agcgatgatc cggagctggc
      781 taatgatgcc atcctgaccg caatttacca agcatggtat gaggaggtaa accccctatg
      841 agtgaagagc tcaacaccca aggctacgat atcccgcctg aattgcaggg acaggtagcc
      901 attaccaccc tggataaaat ctataactgg ggtcggcgtt cctccatctg gccgatgatg
      961 tttggcctgg cgtgctgcgc gattgagatg atctgtactg ctgccagtcg ctatgatctt
     1021 gctcgcttcg gcatggaggt gatgcgcccc agcccgcgcc aggcggacat gatgattgtc
     1081 tcgggcacag tgaccaagaa aatgatcacc accatcgtgc gcttgtataa ccagatgcca
     1141 gaacccaaat acgtggttgc gatgggtgcg tgcgcctctg gtggcggacc gtttaaagag
     1201 ggctataccg tggtctcagg
//
LOCUS       DBNHOH010000746         6858 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1713012, whole genome shotgun sequence.
ACCESSION   DBNHOH010000746 DBNHOH010000000
VERSION     DBNHOH010000746.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6858)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6858)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6858
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1713012"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="AC16V7_13375"
     CDS             <1..353
                     /locus_tag="AC16V7_13375"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR6258159.1"
                     /translation="VRPTNRSGGLEGGITTGQPLHVRAAMKPIATTLTPQKTVDLEKG
                     VETPTQYERSDFCPVPRAVVIIESMVAFVLADALIEKLGGDSLEEMRPRFEALRRARL
                     SDLPMDGSEHVFWP"
     gene            337..1890
                     /gene="aroB"
                     /locus_tag="AC16V7_13380"
     CDS             337..1890
                     /gene="aroB"
                     /locus_tag="AC16V7_13380"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIR6258160.1"
                     /translation="MSSGRDFICLYGPPGSGKSSVGRALAARLGFPFWDLDEEIQVRS
                     KMNIPEIFAREGEKGFRRRERKVLLDLLAHQEGVLALGGGALLNPANLTQVEAAGTVL
                     CLQAPIERLLERLGAESNSRPLLNGDLRPRLESLLSSRADHYNSFPFALDADWPVERI
                     VRDAQIRIGKFRVSGMGQAYDVIVQERGLDGIGDLLLKRGLRSPVIVVSDENVGSLYT
                     QQVLDSLQSANYRAHSITIPPGEQHKNLHTVASLWEGFLAAGIERSSTVIALGGGVVG
                     DLAGFAASTILRGVPWVALPTTLLAMADASLGGKTGFDLPQGKNLVGAFHSPRLVLAD
                     PHTLETLPSAELRAGMAEVVKSGLVGDAELFALCGRVWEAVERDLGEVVRRAMAVKIS
                     VIEDDPYEKGPRAALNLGHTFGHAVELASGFQLRHGEAIAIGLVAEARLAEKIGIAET
                     GLTGEIEGVLRALGLPVEIPPELDRVAVREVMQVDKKRLDGRVRFALPIRPGEVKVGV
                     VVDFTELSI"
     gene            1962..2411
                     /gene="aroQ"
                     /locus_tag="AC16V7_13385"
     CDS             1962..2411
                     /gene="aroQ"
                     /locus_tag="AC16V7_13385"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015170084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HIR6258161.1"
                     /translation="MKPILVLHGPNLNLLGLREPEVYGSLTLEEIDRRLVEVGREMGL
                     EVRALQYNGEGDLIDALHQARDWARGVIFNPGGYTHTSVALRDAVASIGLPVVEVHLS
                     NVHAREEFRRQSMIAPVCVGSIVGFGWRSYWLGLHSLAAILNKSDIK"
     gene            complement(2464..3282)
                     /gene="jag"
                     /locus_tag="AC16V7_13390"
     CDS             complement(2464..3282)
                     /gene="jag"
                     /locus_tag="AC16V7_13390"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HIR6258162.1"
                     /translation="MRKSRTTLEVIAPTIEEAVIKGLDELGLPQDAVDVEILDEGSKG
                     LFGLGSRQARVRLTVKTTSPGEEAGEEKPARAKRAAKRTPQKVSEVQEDVLPSEMEAG
                     EEDESTTAIKAEPQDDYILQIAEAVVAELLHRMRVRADVSAEFGEADDRSHRVPVLVN
                     ITGQDLSILIGRQTETLNALQYIAGLIVSKETGRPLTLIIDVQGYRKRREQQLRQLAR
                     RMADQAVKTGRRQVLEPMPANERRIIHIELRDYPEVSTESIGEEPRRKVTILLK"
     gene            complement(3342..4100)
                     /locus_tag="AC16V7_13395"
     CDS             complement(3342..4100)
                     /locus_tag="AC16V7_13395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HIR6258163.1"
                     /translation="MWDMLIINPMVNTLLWIYSVLWQNFGLAIIVFTLVVRLITYPLT
                     AQQMKSAQAMQDMQKSKKWQDIQKKYKDNREKLAQEQMSLYKEMGVSPFGSCLPTLIQ
                     FPIIIGLYQGIIRALAVTPIQLLDLSTHIYPFINASDLIPLHNRFLWMDLSQPERLYV
                     FGFGVPVLAILVVITTYFQSKLMTPMTQPGDQGAQMTQAMNLYMPFLMGWLAYSFSSG
                     LALYFVASNVFTILQYAAMGKLQWSNLLPTRKSR"
     gene            complement(4115..5215)
                     /locus_tag="AC16V7_13400"
     CDS             complement(4115..5215)
                     /locus_tag="AC16V7_13400"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR6258164.1"
                     /translation="MKSKHLLILIILLISSVVLSACAGGAATAASSWPDLTVDGETAF
                     LSYNQHVYAIDLANGTQKWRFPLEADNKISFYAAPVITPDGQLLVGGYDHVLYSLNPQ
                     TGTENWSFKNAKNRYIGSPLASEAGIFAPTADDNLYALDNQGNARWSFATQGAQWAKP
                     VSDPNCECIYLPSMDHHLYAINAQTGAQEWKSEDLGGSVVGTPAYAPDDTLYIGTFNS
                     EMLAIDANNGRIRWRVTTDGWIWGGPVLKDGALYFGDLSGAFYAMDAADGKVRWKIKP
                     DGPISQSPLLTEESIYFTTEAGSLYGVSYNGDIRVNQPIACQPNGACPKLHTSPVSAG
                     DKILVAPTGVEQLLIALDANGEQIWSFIPEKK"
     gene            complement(5264..5470)
                     /gene="yidD"
                     /locus_tag="AC16V7_13405"
     CDS             complement(5264..5470)
                     /gene="yidD"
                     /locus_tag="AC16V7_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013299151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIR6258165.1"
                     /translation="MILLGLIRLYQMTISRGLPPNTCRFHPSCSHYGYQAIYKYGALK
                     GSLMAAWRVLRCNPFNPGGYDPVK"
     gene            complement(5547..5894)
                     /gene="rnpA"
                     /locus_tag="AC16V7_13410"
     CDS             complement(5547..5894)
                     /gene="rnpA"
                     /locus_tag="AC16V7_13410"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIR6258166.1"
                     /translation="MKRDFRLRKSADFERVRRSGKSFAHPLVVLIVHDNPGEGVRIGV
                     AAGRSLGMAVQRNRAKRMIRAAVHSMLNHIRPGHDLILIARRPMLEADFHKLQEALLT
                     LLRRAQILNANDD"
     gene            complement(5944..6120)
                     /gene="rpmH"
                     /locus_tag="AC16V7_13415"
     CDS             complement(5944..6120)
                     /gene="rpmH"
                     /locus_tag="AC16V7_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIR6258167.1"
                     /translation="MPKRTYQPKIRRRVRVHGFRSRMATAGGRKVIKNRRLKGRSRLG
                     VTKNNHVKKVNWNA"
     gene            complement(6217..>6858)
                     /locus_tag="AC16V7_13420"
     CDS             complement(6217..>6858)
                     /locus_tag="AC16V7_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR6258168.1"
                     /translation="LLNQNHHVHIIENRREVLSRMHRELPTEIIYEGLFSNPAVLEQA
                     EIQKADVLAACTVNDAENLVACFLGRTRYGIGRTIARVNNPRNAWLFDEKFCVDVALN
                     NAEIMARLIEEEMSMGDMMTLLKLRRGDFSLVEEKIPPGAPAIGIAIQNLGLPDNCII
                     AAIVRRGQMLVPRGITTFEAGDEVLAITDSQGAEQLASLFTPSESKSKGSNPI"
BASE COUNT         1507 a         1684 c         1955 g         1712 t
ORIGIN      
        1 tggtgcgacc gacaaaccga tctggagggc tggagggggg gattacaacc gggcaaccac
       61 tgcacgtgcg agctgccatg aaacccatcg ctaccaccct gacgccacaa aaaacggtag
      121 acttggagaa aggggtggag actcctactc aatacgagcg ttccgatttc tgcccggtgc
      181 cgcgcgccgt tgttattatc gaatctatgg ttgcctttgt gttggcggat gcgctgatcg
      241 agaagctggg aggcgattcg ttagaagaga tgcgcccccg cttcgaggct ctgcgccgcg
      301 cccgcctctc tgacctgccc atggatggga gcgagcatgt cttctggccg tgattttatc
      361 tgtctatatg gccctccggg ctcgggcaag agcagcgtag gacgagccct cgcggctaga
      421 ttgggctttc cgttctggga tttggatgaa gaaatccagg tgaggagcaa gatgaatatc
      481 ccggagattt tcgcccggga aggcgagaag ggttttcgcc gccgcgagcg gaaagtcttg
      541 cttgatctgc tggcccacca ggaaggtgtg ctggctttgg gcggtggcgc tctgcttaac
      601 ccagccaatc tcacccaggt cgaagctgcg ggaactgtgt tatgcctgca agctcccatc
      661 gagcgattat tagaacgtct tggcgcggag agcaactctc gtccgctgct aaatggagac
      721 ctgcggcctc gtctggaaag cttgttatcc agccgagccg atcattacaa ctcatttcca
      781 ttcgccctgg atgccgattg gccggttgag cggattgtcc gcgatgctca aatccgcatt
      841 ggcaagttcc gtgtgagtgg tatggggcag gcatatgatg tgattgtcca ggaaagaggt
      901 ttagatggca tcggagattt gcttctcaaa cggggattaa gaagcccggt aattgtagtg
      961 agcgatgaga acgtaggttc gctctatacc caacaagttt tggattcgtt acaatctgca
     1021 aactacaggg cgcattctat taccattcca ccaggagagc agcacaagaa tctacacacc
     1081 gtggcttcct tgtgggaggg ttttctggcc gcggggatcg agcgcagcag tacggtgatt
     1141 gccttgggag gcggagtggt aggtgatttg gcgggatttg ccgcttcgac gatcttgcgc
     1201 ggcgtgccct gggtggcgct gcccacaacg ctactggcaa tggctgatgc cagcctgggc
     1261 ggtaagaccg gatttgacct gccccagggt aaaaacttgg ttggagcctt ccactcgccg
     1321 cgcctcgtgc tggctgaccc gcatacccta gagacattac ccagtgctga attgagagcg
     1381 ggtatggccg aagtggttaa atctggcttg gttggcgatg cggagctgtt cgccctatgt
     1441 ggtcgcgtct gggaggctgt agagcgagat ttgggcgaag tagtgcgcag ggcgatggcg
     1501 gtgaaaatct cggtcattga agacgatccc tatgaaaagg gtccccgggc ggcgttaaac
     1561 ttggggcata ccttcggcca tgctgttgag ctggcgtcgg gctttcaatt gagacacggg
     1621 gaggcaatcg ccattggtct ggttgctgag gcgcgcctgg ctgagaagat tggcattgct
     1681 gagacggggt tgactggcga gatcgaaggt gtcttgaggg cgctaggctt gccggtagag
     1741 attcccccgg agttggaccg tgttgcagtt cgggaggtga tgcaagtgga taaaaaacgt
     1801 ctcgacggaa gagtgcgctt cgctttgcct atccgcccag gcgaggttaa ggtgggtgtg
     1861 gttgtggatt ttacggagct atcgatataa gatatatagg ggaagcaata ctaccagtca
     1921 ggatctgaag aaccttatga tgcagcacca atgagtggaa aatgaaaccg atacttgttt
     1981 tacacggccc aaacctaaat ctacttgggc tccgggagcc cgaggtttat ggctcactga
     2041 cactggaaga gatcgataga cgcttggtcg aggtggggcg agagatgggg ttggaggtgc
     2101 gtgcgctaca atataatggt gagggggatt tgattgatgc cttgcaccag gcgcgcgact
     2161 gggcgcgtgg ggtgattttt aaccccgggg gatacactca cacatcggtg gcgttaaggg
     2221 atgctgtcgc ctcgatcggg ttaccggttg tagaggtgca tctctccaat gtgcatgcaa
     2281 gagaggaatt tcgccgccaa tcgatgatcg cgccggtttg tgtaggatca atcgtgggat
     2341 ttggttggcg ctcttactgg ttgggattgc attccctggc ggcgatacta aacaaatcgg
     2401 atattaagta ggactagagt aaatcgaaga tcgaatcccg aaggggtgaa gaactgagcg
     2461 agtttacttg agcaggatgg tgactttccg tcgcggctct tccccaatac tttctgttga
     2521 aacctcagga tagtcgcgta gctcgatgtg gataatacgg cgctcattgg cgggcattgg
     2581 ctccaagacc tggcggcgcc cggtcttaac agcctggtca gccatacggc gtgcgagctg
     2641 gcgtagctgc tgctcgcgcc gctttcgata gccctgcaca tcaatgatca gcgtcaaggg
     2701 gcgacctgtc tccttgctga cgatcagccc ggcaatgtac tgcaaggcgt ttagggtttc
     2761 tgtctggcgt ccgatgagga tgctcaagtc ttgaccggtg atattcacca gcactggcac
     2821 ccggtgactg cggtcgtcgg cttcaccgaa ttcagcgctg acatcggcac ggacgcgcat
     2881 tctatgaagc agctctgcga caacagcctc agcaatctgg agtatgtaat cgtcttgcgg
     2941 ttctgccttg atcgcagtag ttgattcgtc ttcctctccc gcttccatct cgcttggcaa
     3001 gacgtcttcc tgcacttcgg agaccttctg gggggtgcgt ttggccgcac gtttagcccg
     3061 ggccggcttc tcctcacctg cttcttcacc tggggaggtg gttttgacag ttaaacggac
     3121 gcgtgcctgc cgggaaccca ggccaaatag tcctttgcta ccttcgtcca ggatttcaac
     3181 atccactgca tcttggggta atcctaattc atccagcccc ttgatgacgg cttcctctat
     3241 agttggcgca attacctcta aagttgtacg tgatttccgc atgatggcct cttcaataat
     3301 tgtcctgctt ggtaggacgg gcactaattc ttaagttatc ctcatcggga tttgcgggtt
     3361 ggtagcaggt tgctccattg aagtttgccc atggctgcat actggagaat ggtgaagaca
     3421 ttgctagcca caaagtacag ggctaaccct gatgagaatg aataggccag ccaacccatc
     3481 aggaatggca tgtataaatt catcgcctgg gtcatttggg ctccctggtc gccgggctgc
     3541 gtcattgggg tcatcagctt tgactggaaa tatgtggtga tgaccactaa tattgccagc
     3601 accggcacgc caaatccgaa aacatacagt cgctcaggct ggctgagatc catccacaga
     3661 aaacggttat ggagtggaat caagtcagag gcattgataa atgggtaaat gtgggtggag
     3721 agatcgagca attggatggg ggtaaccgcc agagcgcgta ttatgccctg gtagaggccg
     3781 ataatgatag ggaactggat cagcgtcggc aggcaggagc cgaatgggct aacccccatc
     3841 tccttatata ggctcatttg ctcctgtgcc aatttttcgc ggttatcctt atatttcttt
     3901 tggatatcct gccacttctt cgatttttgc atgtcctgca tcgcctgagc gcttttcatc
     3961 tgctgggccg tgagcggata ggtgatcagg cgcacaacca aagtgaacac gataatcgcc
     4021 aggccaaaat tttgccaaag cacgctgtaa atccatagca gcgtattgac cattgggttg
     4081 ataatcagca tatcccacat atcaatatct tccgttattt cttctcagga atgaatgacc
     4141 agatttgctc gccatttgca tccagggcga tgagtaattg ttctaccccg gtgggtgcca
     4201 ctaagatttt atcacctgcg ctgacagggg atgtgtgcaa cttggggcat gccccgttgg
     4261 gttggcaggc gataggttgg ttgacgcgaa tatcgccatt atagcttaca ccatacagag
     4321 atcccgcctc agttgtgaaa tatatcgact cttcggtgag cagaggagat tgggaaattg
     4381 gaccatccgg tttgattttc cagcgaacct tgccatctgc ggcatccatg gcgtaaaatg
     4441 caccgctcag atcgccgaaa tacagcgcac catcctttaa gactgggcca ccccaaatcc
     4501 atccatccgt agttactcgc cagcgtatgc gaccattatt agcgtcaatc gctaacattt
     4561 cgctgttgaa ggtcccgata taaagtgtat catccggcgc ataagctggg gtccctacga
     4621 ccgatccacc taagtcttcg gatttccatt cctgcgcgcc cgtttgagcg tttatagcgt
     4681 ataaatgatg gtccatagaa gggaggtaga tacactcaca attgggatct gaaaccggct
     4741 ttgcccactg cgcgccctgc gtcgcaaagg accagcgagc atttccctgg ttgtctagag
     4801 cgtagaggtt gtcatcagcg gttggcgcaa aaattcctgc ttcgcttgcc agcgggctgc
     4861 caatatagcg gtttttagcg tttttgaatg accagttttc tgtccctgtc tggggattta
     4921 gactgtatag gacatgatcg taacccccta ccaataactg tccatcaggg gtaatgaccg
     4981 gggcggcgta gaagcttatt ttgttatcgg cttcgagagg gaagcgccac ttctgggtgc
     5041 cgttagctaa gtcaatggca taaacatgct ggttgtatga cagaaaagcg gtctctccgt
     5101 cgactgtcaa gtctggccag ctggaagcgg ctgtggcagc gcccccagca caggcgctta
     5161 gtacgacgga ggaaatgagc aagatgatga gtattagtag atgtttagat ttcatatttc
     5221 gcttataaat tcgaaaatcc tcaggcatgg tttgatccat taattacttt acaggatcgt
     5281 atccacccgg gttgaatgga ttacagcgca aaaccctcca ggcagccatg aggctgcctt
     5341 ttaatgcacc gtacttgtat attgcctgat aaccgtaatg agagcaagat ggatggaagc
     5401 ggcatgtgtt tggtggcagg ccacgcgaga tggtcatttg atatagccgg attaatccca
     5461 gcaagatcat gcgggggata ttttgcaggc tgagaggcag atcgcgcaag cgtggctcag
     5521 atgtatattc gtgtgttgtt tctacttcag tcgtcatttg cattcagtat ctgggcgcgg
     5581 cgtaacagtg tgagcagtgc ctcttgcagc ttatggaagt cagcctcaag catgggacga
     5641 cgagcaatca agatgagatc gtgaccaggg cggatgtgat ttagcataga atgaacggca
     5701 gcgcgtatca tgcgtttcgc tcgattgcgc tggacagcca ttcccaagct acgtccggcg
     5761 gcaactccga tgcgaacacc ctctcctggg ttgtcatgca caatcaacac gacaagcggg
     5821 tgggcaaaag actttcccga ccgcctcacc cgctcgaaat cggctgattt tctcagtcga
     5881 aaatcgcgtt tcacctgtct gacggagcca gatcatgctg atgtttcatc cgctcctgcc
     5941 tgattatgca ttccaattaa ccttcttgac gtgattgttc ttggtaacgc ctaagcggct
     6001 gcggcctttc aagcggcggt tcttaatcac tttacggccc ccggcagttg ccatgcggct
     6061 acgaaaaccg tggacgcgca ctctgcggcg tattttgggc tggtaagttc gtttcggcat
     6121 agttgtaatt ccgttcctaa gttaatgata gcagcattta ttataaactg tacgataaca
     6181 gacgaaaggc gattataccc tatggaagaa gtttggtcaa atgggattcg atcccttcga
     6241 ttttgattcg gagggggtga acaagcttgc caattgctcc gctccctgac tgtcggtgat
     6301 cgccaatacc tcatcgccag cttcgaaggt cgtgatgcca cgtggcacga gcatctgacc
     6361 ccgtcggacg atggccgcga taatgcagtt atccggaaga cccaaatttt ggatggcgat
     6421 gccgatggct ggtgcccctg gcggaatttt ttcctcaacc aatgagaagt caccgcggcg
     6481 cagcttcagc aaggtcatca tgtcgcccat cgacatctct tcctcgatta accttgccat
     6541 gatctcggcg ttatttagag cgacgtccac gcagaatttt tcatcgaaaa gccaggcgtt
     6601 gcgtgggtta ttgactcgcg cgattgtgcg cccgatcccg tatcgggtgc gccccaggaa
     6661 gcaggctacc aggttctctg catcgttgac tgtacatgca gcgagcacat ctgccttctg
     6721 gatctcagcc tgttcaagca cagctgggtt ggaaaagagc ccttcgtata tgatctcggt
     6781 gggcaactcg cggtgcatgc ggctaagaac ttcacggcga ttttcgatga tgtggacatg
     6841 gtggttctga ttaagcaa
//
LOCUS       DBNHOH010000747         1034 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_366546, whole genome shotgun sequence.
ACCESSION   DBNHOH010000747 DBNHOH010000000
VERSION     DBNHOH010000747.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_366546"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="AC16V7_13425"
     CDS             <1..547
                     /locus_tag="AC16V7_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR6258169.1"
                     /translation="VIVVNGVSKLYGMTGFRIGWVVTSRKLAEVITNVQAQTTSCTSV
                     VLQAAAEGALMGMQSIVENLRLTFENNRDVIMQELSSFNGIRVIKPNGTFYILPDFRA
                     YCPSSLAKNSVELSKFLLKKALVVTIPGKEFGMEGYLRLSFSGSVKDITTGIERIKWA
                     LDPQAPNEIYIGDRKLVRDWL"
     gene            544..>1034
                     /locus_tag="AC16V7_13430"
     CDS             544..>1034
                     /locus_tag="AC16V7_13430"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HIR6258170.1"
                     /translation="MNNILEIKTPAQSQASALKCDYGLESLGLMNLNRAYWNLPTEAL
                     YEEAVFRNEGKITRQGSIVVNTGIHTARAANDKMIVRESSSENNIWWGQYNRPYNPEN
                     FNDLFNRVQGYLQGRDIFIQDCYAGADPNYRIPIRIITEYAWHSIFARNMFLIPKTTE
                     DYYR"
BASE COUNT          280 a          250 c          253 g          251 t
ORIGIN      
        1 ggtgatcgtg gttaacgggg tctcgaaact ctacgggatg accggtttcc gtatcggttg
       61 ggtagttacc agccgcaaac tagccgaagt catcaccaac gtgcaggcac agaccacctc
      121 atgtacatcg gttgtccttc aggcggctgc cgaaggcgcg ctgatgggca tgcagagcat
      181 cgtcgagaac ttgcgcctaa cttttgaaaa taatcgcgat gtgattatgc aagagttgtc
      241 ctcttttaat gggatacgcg tcatcaagcc gaatggtact ttttacatcc tgccagattt
      301 ccgagcatat tgcccctcta gtctcgccaa gaattctgta gagctgtcta agttcttact
      361 caagaaagcc ctggttgtga ccattcccgg aaaagaattt ggaatggaag gctaccttcg
      421 cttgagtttt tccggtagtg tcaaggatat tactactggt atcgagcgaa taaagtgggc
      481 attagatccg caagcgccca acgagatata catcggtgat cgtaagctgg tgagagattg
      541 gctatgaaca acatcttgga gatcaaaaca cctgcccaat cacaagcgtc agcactcaag
      601 tgtgattatg gcctagagag cctggggttg atgaatctca atcgggcata ctggaatctg
      661 ccaacagaag cgctatatga agaggcagta ttccgcaatg agggaaaaat cacccgccaa
      721 gggtcaattg tcgttaacac tggcattcac acggcgcgcg cagccaatga caagatgatc
      781 gtgcgcgaaa gcagctctga gaacaatatt tggtggggtc aatacaaccg cccttataac
      841 ccagagaatt ttaacgactt atttaatcgc gtccagggat acttacaagg tcgagacatt
      901 tttatccagg attgctacgc gggtgctgat cccaattacc gcattcccat tcgcatcatt
      961 acagagtacg cttggcacag catattcgcc cgcaacatgt tcctgatccc aaagaccaca
     1021 gaggactatt accg
//
LOCUS       DBNHOH010000748         3187 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_46036, whole genome shotgun sequence.
ACCESSION   DBNHOH010000748 DBNHOH010000000
VERSION     DBNHOH010000748.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_46036"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..268)
                     /locus_tag="AC16V7_13435"
     CDS             complement(125..268)
                     /locus_tag="AC16V7_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258171.1"
                     /translation="MLMGVDRKEYIFEGNKLGELLEAVFAEYDLRDLMLDEEGSIRPW
                     AIE"
     gene            complement(311..589)
                     /locus_tag="AC16V7_13440"
     CDS             complement(311..589)
                     /locus_tag="AC16V7_13440"
                     /inference="COORDINATES: protein motif:HMM:NF041918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HIR6258172.1"
                     /translation="MKVNFYATLRQIVGTKIVEVDVPEGITIRELLLEVTRRHPALSR
                     EMFDKSGNLHAHIHVFINGREYSFIEGNLEARVKLSDTISVFPAVGGG"
     gene            complement(615..1766)
                     /locus_tag="AC16V7_13445"
     CDS             complement(615..1766)
                     /locus_tag="AC16V7_13445"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR6258173.1"
                     /translation="MTEISLDSERQKQAKIYARIQRRLMLVDLALGTVYLIAWLVFGW
                     SAALKGILSQWTANEWYLVAAYGLVFGAGIYVLGLPLSYYSGFILPHRFELSNQTLIG
                     WISDHVKSLLIGGLLGGFLLEVVYAILRATPDLWWLWVAGFLLVFNVLLANLAPVLLF
                     PIFYKFVPLGDEHVDLAERLVRLAERAGARVQGVFKFDMSRRTKAANAALAGLGNTRR
                     IILGDTLISEFNPDEIETVLAHELGHHVNKDIPIGIVIESLMTLIGLFLASLGLKWGV
                     VIFGFSSTADIAALPVLALVMGAYGLITMPLGNAYSRWRERRADEYALQATSNGEAYA
                     SALTRLANQNLADADPEPWVEFLLYSHPALSKRIAMANATAPVAIENSE"
     gene            complement(1849..>3187)
                     /locus_tag="AC16V7_13450"
     CDS             complement(1849..>3187)
                     /locus_tag="AC16V7_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258174.1"
                     /translation="TFSNDLPIRRIIQTWWLLAASWLMMSLEGPALSAVVARLSQPEI
                     NLAAYGGVVFPLALIIEAPIIMMLAASTALSKDWDSYWRLRRYMMFAGLSLTVLHLLV
                     AFTPLYYVVVRDLIGVPSEILEPARIGLMIMTPWTFSIAYRRFNQGVLIRFGRSQAVG
                     IGTVVRLSTNIGVLIVGFALKTVPGIVVGACAASAGVISEAAYAGIAVRPVIRGPLKL
                     APPVAQALNLRSFLDFYIPLAMTSLLLLVAQPMGSAAISRLPMALNSLAVWPVVSGLI
                     FIFRSFGIAYNEVVVALLDEPRSYTNLRRFTHWMAAILSLLFLAVAATPLAHFWFESI
                     SALPSELVALGQQSLWFALPLPALAVWQSWLQGAILHGKHTGAISEAVIIYMSSMAVL
                     LFASVAWGQMVGINAALLAMGVSMALQTAWLWWRSRSMLRDVANRDAALAAQA"
BASE COUNT          888 a          906 c          741 g          652 t
ORIGIN      
        1 gcctgagcag ttactaaatt ttttcagaac gcaaccacat aagggtgcag gagggctatc
       61 aaatctccct cctgaatagt tgcgttcata tccccgattg tataagaaaa gcgaccattt
      121 acagctattc tatagcccag ggtctgatgc tgccttcttc atccagcatt aaatcgcgca
      181 ggtcgtattc agcaaagact gcctctaata actcgccaag cttgttcccc tcgaagatat
      241 attctttcct gtcgacaccc atcagcatgc gcacatcccc agtgaacttc agtacaactc
      301 gagtctacgg ttagcctcct cccaccgcag ggaacacact gatggtatcc gacagtttca
      361 ctcgagcctc cagattacct tcaatgaacg aatattcccg accgttgatg aatacatgga
      421 tatgcgcgtg caagttaccg ctcttatcga acatctctcg agacaaggct ggatgtcggc
      481 gagtaacttc taggagcagc tcccggatcg taattccctc gggcacatcg acctccacga
      541 tcttggtgcc cactatttgg cgcagagttg catagaaatt tactttcata tccttctgag
      601 gttagtatac attatcattc actattttca atagcaaccg gggcagttgc attagccatc
      661 gcgatgcgct tgctcaaggc agggtgagag tacagcagga actctaccca gggttctgga
      721 tcggcatccg ccaggttctg gttcgctagg cgggttaacg ccgatgcata ggcttcgccg
      781 tttgaagtag cttgtagagc atattcatct gcccgccgct cccgccagcg cgaataggca
      841 ttgcctagag gcatggtgat cagcccatag gcacccatca ccagcgccaa gaccggcaag
      901 gcggcgatgt ccgccgtgct gctgaatcca aatatcacaa ccccccactt cagtcccaat
      961 gaagccagga acaagcctat aagtgtcatt aaactttcga tgacgatgcc tatcgggata
     1021 tctttattga catggtggcc tagctcatgg gccagcacag tctcgatctc atctgggttg
     1081 aactcactga tcaatgtatc gcccaggatg atccgccgtg tattacccaa ccctgcaagg
     1141 gcggcattgg cggctttcgt cctacggctc atatcaaact tgaacacacc ctgcacgcgc
     1201 gcaccggctc gctcagccag gcgaaccagt cgctcagcca gatcaacgtg ctcatctccc
     1261 agggggacaa atttataaaa gatcgggaat agcagcaccg gtgccaggtt agccagtaga
     1321 acattaaaca cgagcaggaa tccggctacc cacaaccacc atagatcggg tgtagccctc
     1381 aatatggcgt agacaacttc caaaagaaaa ccgcccaaca acccaccgat taacaaggat
     1441 ttcacatgat cgctaatcca accgataagg gtctggttgg aaagttcaaa acggtgtggc
     1501 aggataaagc cactgtaata cgaaaggggc aagccaagca cataaatgcc ggcgccaaaa
     1561 accagtccgt aagcagcgac caagtaccac tcgtttgcag tccactggga caaaatacct
     1621 ttaagcgctg ccgaccagcc aaaaaccagc caggcgatga ggtacacagt ccccaaggcc
     1681 aggtccacca gcatcaggcg gcgttggatg cgggcataaa ttttcgcctg cttttggcgc
     1741 tcagaatcaa ggctaatttc agtcatggcg agattatatc ttgaaaccaa atgagaatcg
     1801 gtagtgcatc catactccaa caaactatta aagacttctt ctatgtatct acgcttgagc
     1861 cgccaatgcg gcgtcgcggt tagccacgtc tcgcaacata gaccggcttc gccaccaaag
     1921 ccatgcggtc tgcagagcca tactgacccc cattgccaac aaggcagcgt ttatgccaac
     1981 catctgcccc caggcaacgc tcgcgaagag taaaactgcc atggatgaca tatagatgat
     2041 caccgcttca gagatagccc cggtatgctt tccgtgcaat atggcgccct gcaaccagct
     2101 ctgccatacc gcaagggcgg gcaggggaag agcaaaccat aagctttgct gtcctaatgc
     2161 caccagttca gagggcaggg cagagatact ctcgaaccag aaatgtgcca gtggtgtcgc
     2221 agccacagcc agaaacaaca ggctcaaaat tgccgccatc caatgcgtaa agcgacgcag
     2281 gttggtatac gagcgaggtt cgtccaacaa agccaccacc acttcattat aggcaatacc
     2341 aaagctgcgg aaaataaata tcaagcccga aaccaccggc caaaccgcca gtgaattcaa
     2401 tgccatcggc aggcgactaa tagcagcact acccataggc tgggcgacca ggagcaggag
     2461 cgacgtcatc gccaggggga tataaaaatc aagaaagctg cgcaggttaa gtgcttgcgc
     2521 tacagggggt gccagcttca atggtcctct tatcaccggt cgcaccgcaa taccagcata
     2581 ggcagcctcg ctaatcacgc ccgcgcttgc agcacatgct ccaaccacga taccaggaac
     2641 agtttttaaa gcaaaaccaa ctatcaatac accgatattg gtgctcaatc gcacaactgt
     2701 acctattcca actgcctggg agcgaccaaa gcggattaaa acaccctggt taaaacgccg
     2761 ataagcgatc gagaatgtcc aaggggtcat aatcatcaat ccgatacggg ctggttctag
     2821 gatctcactt ggcacaccga tcagatccct gacgacgaca taataaagag gtgtaaaggc
     2881 tactaaaagg tgcaaaactg tcaatgagag gccggcaaac atcatatacc ggcgcaaacg
     2941 ccaatacgaa tcccaatctt tgctcagagc agttgaagcc gccagcatca tgatgatagg
     3001 cgcctcaatg atcaaggcca gggggaagac caccccaccg tatgccgcca aattgatctc
     3061 gggctgcgaa agccgcgcca caactgcgct cagcgctgga ccttccaagc tcatcatcag
     3121 ccagctagca gccaacagcc accaagtttg gatgatgcgc cgaatgggta gatcatttga
     3181 aaaagtc
//
LOCUS       DBNHOH010000749        10433 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_366755, whole genome shotgun sequence.
ACCESSION   DBNHOH010000749 DBNHOH010000000
VERSION     DBNHOH010000749.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10433)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10433)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10433
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_366755"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="AC16V7_13455"
     CDS             <1..229
                     /locus_tag="AC16V7_13455"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR6258175.1"
                     /translation="FDEVLRLHSRLGKLGNTSMRFEFQIFSEEDQRLVATGEITVVIA
                     QSQSYQKTPIPSRLRQAVAAYEEDPTLMNSA"
     gene            259..1281
                     /locus_tag="AC16V7_13460"
     CDS             259..1281
                     /locus_tag="AC16V7_13460"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR6258176.1"
                     /translation="MKAAVFYGGDQPLKIEELPNPEPAPGEVLVKIAACGLCHTDLHY
                     LDHGVPTFKKPPLVLGHEVSGTIAGLGAEVEQWKEGDRVLLPAVYGCGQCAMCREGRE
                     NICERMVMFGNNVDGGYAEYMLAPAKDALRLPEEVPLVEGAIIADAITTPYHAVVNRG
                     QVKPGDYVVVFGCGGIGLNVIQVAAALGAQVIAVDIIDTKLEWAKQLGAQVALNAQNF
                     ERIDKEIRKLTGGGADKGFEAIGNPVVQAQTFASLRTGGRFVVVGFSAKPMTLDTGRV
                     MYREMEIIGSLGCRAVDYPRVIDLVRQGKIKVKELVTARYPLAEINEAFDGLRRGEGI
                     RSVVVP"
     gene            1278..2168
                     /locus_tag="AC16V7_13465"
     CDS             1278..2168
                     /locus_tag="AC16V7_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6258177.1"
                     /translation="MIIDFHIHLSRPEHELPWVMEWIRSNFSGDIDAFVEEVLTPSGL
                     RRTLQANHIDWAVGLAEVSPVTTGWASNEYVGELCMQTNALPDPPSGPRGRLIPFASI
                     NPFIVNDLGAELTRLVDEYGFGGVKVYPTYHHHYPNDARLSPLYARAQELGLPVLVHT
                     GSSVFKGTRIKYGDPLLLDDVANDFPNLKILMAHSGRPFWYEQAFWMARQHSNVYMEV
                     SGLPGKKLLEYFPRLEEITDKVVYGSDWPGNPDFRRNVEAIMSLPITEQAKQDILFNN
                     AARILGVSEREGHSTSERPS"
     gene            2209..3957
                     /gene="iorA"
                     /locus_tag="AC16V7_13470"
     CDS             2209..3957
                     /gene="iorA"
                     /locus_tag="AC16V7_13470"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019224841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HIR6258178.1"
                     /translation="MRELLLGNQAIARGAWEAGVTFAAGYPGTPSTEIIETLATRFTE
                     TNPRWSANEKVAFEEGMGAAIGGLRVLVTMKHVGLNVAADAFMVFPYAGTNGGFVVVS
                     ADDPGMHSSQNEQDNRYLARMGKVPVLEPSDAQECKDFVIAGLELSEKYGIPVMLRTT
                     TRLAHHKGLVEIGERKDVPKRDFVPDAKRFSVPIYRRLHRPKVEERLQALKEYAETSP
                     LHRIEHTENPVGVVTSGIAYQYVKEVMPEAGVLRLGVTFPLPENLLRDFCSRYEKVYI
                     VEEGEPFIEEFMRSLGITNLVGKELFGVIGEYSPGRLMEALGMGKTPEPFTEGITLLP
                     RPPMLCIGCGHRTVFASLRQLKVTVAGDIGCYTMGALPPYEAEHTTFCMGASIGTAVG
                     LERAGYKRVVAVIGDSTFVHAGIPPLIDAVYNGSNLNLIILDNSATGMTGSQPHPASG
                     QDAQGRPAPKLNLVEVVRAAGVEHVKVVDTWQRKEVLRSIQQALANDGPSVVIAQGPC
                     QQLPEMKRREVIPYVVEEELCTQCEACFKVWCPAIKRNENNFPYIVANECTACTVCAQ
                     VCPTDAIHLADVQVTA"
     gene            3972..4598
                     /locus_tag="AC16V7_13475"
     CDS             3972..4598
                     /locus_tag="AC16V7_13475"
                     /inference="COORDINATES: protein motif:HMM:NF013707.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HIR6258179.1"
                     /translation="MSKVYSLIFAGVGGQGSLLIADATSLAAVQAGYDAKQTEVHGVS
                     QRGGSVETHVRFGEKVYSPLVTAGQADVVVGLEKLEALRFGHFANPEYGVILANDFEI
                     VPASVQGAEQLYPHETVDYLKNKSLRVITMPASAIARDLGDGRMANVVILGGLSKLLP
                     IPLDIWDKTLHLRIPKRYLSPNLQAFEIGRSQIDVAEGFVSTNTEQKV"
     gene            4651..5958
                     /locus_tag="AC16V7_13480"
     CDS             4651..5958
                     /locus_tag="AC16V7_13480"
                     /inference="COORDINATES: protein motif:HMM:NF012885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LeuA family protein"
                     /protein_id="HIR6258180.1"
                     /translation="MSGTTNDDVSQLIYDWNEIEEFQPPKRRILVTDETLRDGLQSPS
                     ITHPDIQDKVYLLYLMRDLGIDAADLGLCGAGERFKYDVIVLAKEIANQNMPIQPQSA
                     ARTMLDDITPIVDASQEAGIPIEACIFLGTSPIRQYAEGWDVEFLLERTKESVSFATN
                     HGLPVMFVTEDTTRAHPDTLRRVYTAAIEAGAQRLCVSDTVGHATPEGVRALVSYLRK
                     IVDEINPEVGIDWHGHRDRGMDIINTLVAIEAGADRVHACGLGVGERSGNTPMELLLV
                     NLNLMGLATRDLRRLPEYCNIIAEKCGAPVPFNYPVVGADSFRTSTGVHAAAVVKALS
                     RDENWLADRVYCGVPASMVGRVQGIEIGPMSGEHNVRFYLHQRGIEEHPVYVEKILAA
                     AKRSKRLLSEEDVLHMVAVMRERLKSGAEIKESEIEDTLLTRS"
     gene            6168..7508
                     /locus_tag="AC16V7_13485"
     CDS             6168..7508
                     /locus_tag="AC16V7_13485"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6258181.1"
                     /translation="MKGKLNTLFALLAIVALLLASCAQATPEPTEEPPPPPPTSAPEP
                     TEPPPPTEAPAMTEEPTPEPTEEVVGETYKIGFAPAITGPGSSLGAPEHETGQMIAEQ
                     LNAQGGVVGPDGVRHPVEIVIFDTETNPDVAVSVVRRMIEEENVQVVVAGTTSGISMA
                     IVPIITEAEVPYLSMASSGAIVKDPETGEVRKWAFKTAQDNLASAQWQALYLNKLGYT
                     KVCHLSENSGYGGDTLAQAQKAFEPAGIEIVYSDSFERTGTEFPQVINVQTSDCQAVV
                     VGAIPPASSNISIALRDALPDMPIIHGHGTCNQDHIDLMGAEVAEGMVSPCGRVMVSE
                     TLPDDDPQKDVLVQYVTDYTAYTGKPVNTFGGHGWDAVMLAVTAVSSLPDGLDLAAQR
                     AAVRDFLETGVKDWPGISGVYNLSPEDHFSLGFESLTFVKVEGGKWIYFPEEEW"
     gene            7571..8599
                     /locus_tag="AC16V7_13490"
     CDS             7571..8599
                     /locus_tag="AC16V7_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6258182.1"
                     /translation="MEQQSRRKLVQTIIFLGIFLALILLTVFLGGRKNFGLQQYAQLT
                     IDGLRGGAIYALIAMGFVMVFNVTGIINFAQGGFVMLGAMLAVTIYEWELPLPAWMHL
                     LISVLLAILLTSLIGILVERLSIHPARSSSTLTLIIITVGIYIATQGLALLTWGADAY
                     IYPAFTTLEMADRTIRWMGIVIKSQSFWIWGITALVLIALAFFFERTLIGKAMRACAV
                     NRRGARLVGISTSRMSVVAFALAAALGAIGGIVVAPILRPSYDMGLSLGLKGFVAAIL
                     GGLVSTPGAVLGGLMLGLIENLAAGVTKAGMKDIYAFILLILALLFRPQGLLGVRKTA
                     VEADGEDH"
     gene            8599..9714
                     /locus_tag="AC16V7_13495"
     CDS             8599..9714
                     /locus_tag="AC16V7_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6258183.1"
                     /translation="MEPTAKSAPNQNLLGWLLLTVVVGVLGWVELASPFGLDFSDITR
                     GMVTLDTMIRVAILTIVLVGLNLLMGYAGQVSLGHAAFYGMGAYASAIFTARAGSLFG
                     APESLINSWWWPWLVMVLGMIFAGILAYLIGRPILRLRGHYLAMATLGLGVMIYILFR
                     ENMGFQGQHITGTFDGIPGIPRLRLGSFEIWPISRYYFFVWLVALLVIAFGLNLINSR
                     TGRALRAIHGSEVAAQAMGVDVSAYKARIFALSAILASLAGSLYAHFQASVSPAPFSF
                     IGSLELVVMSAVGGLSSIWGAPFGVAVILITKEILRSQLRLLMEGASGEHEIIFYGLI
                     LVVIMIFMPEGVTVGTVRRWRAWRNRSYEKQAQAAQS"
     gene            9727..>10433
                     /locus_tag="AC16V7_13500"
     CDS             9727..>10433
                     /locus_tag="AC16V7_13500"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6258184.1"
                     /translation="MEKKRTNTNLLSIKELRKWFGGLMAVNRISFDVKHGEIVALIGP
                     NGAGKTTIFNLITGFHPIDDGEIRFNEKRIDGLNPHKIVQEGIVRTFQNLQVFNHMSV
                     LENVKVGLHLQGHSGMITSALRMPSARQEEREFHQKALHYLEQVGLVERANDLARNLP
                     FGQQRLLEIARALAVQPQLLLLDEPAAGLTVPETNNLDELICRLQENGLTILLVEHDM
                     GLVMGIADRVVVLHYGT"
BASE COUNT         2407 a         2759 c         2961 g         2306 t
ORIGIN      
        1 ttttgatgaa gtgctgcgtt tgcactcccg gctggggaag cttggcaaca ctagcatgcg
       61 cttcgaattt caaattttta gcgaagaaga tcaacgcctg gtggctaccg gcgagatcac
      121 agtggtcatc gcgcaaagcc agtcctatca aaaaaccccc atcccttcac gcctgcggca
      181 agcggtagct gcctatgagg aagacccaac gctgatgaac tctgcctaat taacaagtcc
      241 tatggagaca tgctaaccat gaaagctgct gttttttacg gaggcgacca gccactaaaa
      301 attgaggaat tgccaaaccc tgaacccgcc ccgggagagg tgctggtgaa aatcgcggcg
      361 tgcggcttat gccacaccga cttgcattac cttgaccatg gagtcccaac atttaagaaa
      421 cccccgctag tactgggaca cgaagtctca ggcacgattg caggcttggg ggctgaggta
      481 gagcaatgga aagaaggaga ccgcgtgctg ctgccggctg tttatggttg tgggcagtgc
      541 gccatgtgcc gtgaagggcg agagaacatc tgcgaaagga tggtgatgtt cggcaacaac
      601 gttgacggag gctacgcaga gtacatgctg gccccagcta aagatgcgct ccgcctcccc
      661 gaggaagtac ccctggtgga gggagccatc atcgccgatg ccatcaccac cccctatcat
      721 gccgtcgtca accgcggtca ggtaaaaccg ggcgactatg tggtcgtctt cggctgtggc
      781 gggatcggct tgaacgttat ccaggtagcg gcggcactgg gcgcgcaagt gatcgcagtg
      841 gacattatcg acaccaagct ggagtgggcc aaacaactcg gtgcacaggt ggcgctcaac
      901 gcccagaatt tcgaacgtat tgataaagaa atccgcaagt tgacgggcgg cggtgctgac
      961 aaaggctttg aagccatcgg caacccggtt gttcaggcgc aaacctttgc ctcgctgcgc
     1021 actggtgggc gcttcgtggt tgtgggattc tccgccaaac cgatgacgct agatactggc
     1081 agggtaatgt accgtgaaat ggagatcatc ggctctttgg gctgtcgagc ggttgactat
     1141 ccacgcgtta tcgacctggt tcgccaggga aagatcaagg tcaaagagct ggtcacagct
     1201 cgctatccac tagcagagat caatgaggcc tttgacggtt tgagaagagg cgagggtatt
     1261 cgcagcgtag ttgtgccatg attattgact ttcacattca tctcagccgt cctgagcatg
     1321 aattaccgtg ggttatggag tggatccgat caaacttttc gggagacatc gatgcttttg
     1381 tcgaagaagt gctgactcct tctgggctgc ggcgcaccct gcaagccaac cacatcgatt
     1441 gggcggtggg attggcggag gtatccccag tcactaccgg gtgggcgagc aacgagtacg
     1501 tgggcgagct atgcatgcag accaacgcgc tccccgatcc cccctcaggg ccgcgcggcc
     1561 ggctgatccc gtttgccagc atcaacccat tcattgtcaa cgatctaggc gccgagctga
     1621 cacgcctggt ggacgagtat ggctttgggg gcgtcaaagt gtacccaacc taccaccacc
     1681 actatcccaa cgacgcacgg ctatcgcctc tttacgcccg cgcccaagaa ctggggctgc
     1741 ctgtcttggt gcacaccggc tcttcggtat tcaaggggac gcgcatcaag tatggcgacc
     1801 cactgctgct ggacgacgtc gccaacgatt tccccaatct aaagatctta atggctcatt
     1861 ctggacgacc cttctggtac gagcaggcgt tttggatggc gcgccagcat tcaaacgtgt
     1921 acatggaagt ctccggtctg ccaggcaaga aactcctgga atacttcccc cgcctggagg
     1981 agatcaccga caaagtagtc tatggctcag attggcccgg caaccccgat ttccgccgca
     2041 acgtagaagc cataatgtcc ttacctataa cggagcaagc caagcaggat attttattca
     2101 acaacgccgc caggatttta ggcgtttcag agcgtgaagg tcactccacc agtgagcgac
     2161 cctcctaatc ggtagcgagc tggagtattg cattttgaag gagagtctat gcgagaatta
     2221 ttgttaggca atcaggcgat cgcccgaggc gcatgggaag ccggggtaac cttcgccgcg
     2281 ggatatccag gcactcccag caccgagatt attgagacgc tggcaacgcg ctttactgag
     2341 accaacccgc gctggtcggc gaatgagaag gtagcatttg aggaaggtat gggagccgct
     2401 atcggcggat tgcgcgtcct ggtgaccatg aagcacgttg gcctgaacgt cgcagcggat
     2461 gccttcatgg tattccccta cgcaggcact aacggcggtt ttgtggtcgt gtctgccgat
     2521 gacccaggga tgcactcctc ccaaaacgag caagataatc gttacctggc aagaatgggg
     2581 aaagtccccg ttttggagcc aagcgatgcc caggaatgca aggactttgt tatcgccggt
     2641 ctcgagctgt ccgagaagta tggcattcca gtcatgctgc gcaccaccac gcgcctggcg
     2701 caccacaagg gtctggtgga gatcggtgag cgcaaagatg tgccgaagcg ggattttgtc
     2761 ccagatgcga agcggttttc cgttcctatt taccggcgct tgcacaggcc caaagttgaa
     2821 gagcgcttgc aagctctcaa agagtacgcc gaaaccagcc ctctgcaccg tatcgaacac
     2881 acagaaaacc cggtgggggt cgtcacttcg gggatagcct accaatatgt aaaagaggtg
     2941 atgccagaag ccggcgtgct ccgcctgggg gtgactttcc cgctaccaga aaatttgctg
     3001 cgcgacttct gctcccgcta cgagaaagtg tatatcgttg aggaaggcga gccgttcatc
     3061 gaagagttta tgcgcagcct ggggataaca aacctggtgg gcaaggagct ctttggagtc
     3121 atcggcgagt attctcccgg gcggctgatg gaagccctgg gcatgggaaa gaccccagag
     3181 ccattcactg aaggcatcac actgctgcca aggccaccta tgctgtgcat cggttgcggt
     3241 catcgcacgg tctttgcttc tttgcgccag ctaaaagtaa ccgttgctgg ggatatcggt
     3301 tgctacacta tgggcgcttt acccccttac gaagctgaac acaccacatt ttgcatggga
     3361 gccagcatcg gcaccgcggt gggtctcgag cgagcgggtt ataagcgggt cgtggcagtc
     3421 attggagatt ccacctttgt acacgccggg atcccaccat taattgatgc ggtgtacaac
     3481 ggcagcaacc tgaacctgat catcctggat aactcagcca ccggtatgac cggcagccag
     3541 ccacatcccg cttctgggca agatgcccag ggcagacccg ccccaaaact aaacctggta
     3601 gaggtggtgc gcgctgcagg tgtggagcac gtcaaagtgg tggatacctg gcaacggaaa
     3661 gaggtgctgc ggtcgattca gcaagccctg gcaaatgatg gcccttcggt ggtcatcgcc
     3721 caaggacctt gccagcagct acccgaaatg aaacgccgcg aagtcatccc ttatgtggtg
     3781 gaagaagagc tgtgcactca gtgcgaggcc tgttttaagg tgtggtgccc ggcgatcaaa
     3841 cgcaacgaga acaacttccc ctacatcgtg gcgaacgagt gcactgcctg cacagtctgc
     3901 gctcaggtat gccctaccga cgccattcat ctggcagacg tacaagtaac cgcatgaggg
     3961 aggcaaacga gatgagtaaa gtatatagtt tgatattcgc cggggttggt ggacagggct
     4021 ctttactgat tgcagatgcc acctcgctgg ctgctgtaca ggctggttat gatgcgaagc
     4081 aaaccgaagt ccacggcgtc tcacaacgtg gcggcagcgt tgaaactcac gttcgctttg
     4141 gtgagaaggt gtattctccc ctcgtcaccg caggccaagc ggatgtggtt gtcggtctgg
     4201 aaaagttaga agccctgcgc ttcggccatt tcgctaaccc agagtatggc gtaatcctgg
     4261 caaacgattt tgagatcgtt cctgcctcgg tgcaaggggc tgagcagctt tacccccatg
     4321 aaacagtcga ttacttgaag aataaatcgc tgcgggtgat cactatgcca gcatctgcta
     4381 tcgcccgcga tctgggcgat ggtcgcatgg caaatgtagt catcctggga ggattatcca
     4441 aactcctgcc tattcccttg gatatctggg ataagacact gcatttgcgc attcccaaac
     4501 gctacttgag ccccaatcta caagcctttg aaattggccg cagccaaata gacgttgcag
     4561 aaggatttgt atctacgaac acagaacaaa aagtataggt aaaccatgag ggcagtcaat
     4621 ttccctcact cacagaaaaa ggaggtagca atgtccggta caacaaatga tgatgtaagc
     4681 caattgatct atgactggaa cgaaattgag gaatttcagc ccccaaagag gcgcatcctt
     4741 gtgacagatg aaacgctgcg cgatggattg caatcgcctt cgatcaccca tcccgatata
     4801 caggataagg tttatctcct gtacctgatg cgagatctgg gcatcgacgc agccgacctt
     4861 gggctgtgcg gcgcgggcga gcgcttcaag tacgacgtga tcgttttggc gaaagaaatt
     4921 gccaaccaga atatgcccat ccagccgcag agtgcggcgc gcacaatgct ggatgacatc
     4981 acgcccatcg ttgatgcctc tcaggaagcc ggcatcccca ttgaggcgtg tatctttctg
     5041 ggaaccagcc caatccggca atacgcagag ggctgggatg tggagttctt gctggaaaga
     5101 acgaaagaat cggtatcctt tgccaccaat catggcttac cggtcatgtt tgtcaccgag
     5161 gacaccaccc gagcccatcc cgataccttg cggagggtct ataccgcggc catagaagcc
     5221 ggagctcagc gcctgtgcgt ctcagatact gtcgggcacg ccacgcccga aggcgtgcga
     5281 gccctggtct cgtacctgcg taaaatagtt gatgagatca atcctgaggt aggtatcgac
     5341 tggcatggac accgggatcg cggcatggac attatcaata ccctggtggc gatcgaagct
     5401 ggcgcagacc gggtgcatgc ctgcggttta ggagttggcg aacgcagcgg gaatactccg
     5461 atggagctgc tcctggttaa cttaaatctc atggggttag caactcgcga cctaagacgc
     5521 ctgccagaat actgcaacat catcgcggag aagtgcggcg ccccagtacc ctttaactat
     5581 ccggtggtgg gggctgattc cttccgcact tcaaccggtg tccacgcagc cgcggtggtg
     5641 aaggccctca gccgggacga aaattggctg gcggaccgag tctactgcgg agttcccgct
     5701 tccatggtgg ggcgtgtaca gggcatcgaa atcggtccca tgtctgggga gcacaacgtg
     5761 cgcttctacc tgcaccagcg tggcatcgaa gagcaccccg tatatgtcga gaaaattcta
     5821 gcggctgcga agcgttctaa acgtctgcta tccgaggagg atgtactcca catggttgcg
     5881 gtgatgcgag aacgcttgaa gagcggagcg gagatcaaag aatccgaaat agaggacaca
     5941 ctgctgacgc ggtcatgatc atgtgtaggg atttttcaaa aggaatgcca taagcatatc
     6001 gctttgagaa atcttaaaac cctgaacaag actattaagg taggtccacc gcaaccatga
     6061 acgcttgtgt gaaatagaaa ggaggtgaaa gactcgtcgt gaactgtgtg agctcaagaa
     6121 agatgcatct attggcacaa ttctgatcga gaaattggag gaaaccgatg aagggaaaac
     6181 taaatactct atttgccttg ttggcgatcg tggcgcttct cctggcatcg tgcgctcagg
     6241 caacacctga acccactgaa gagccaccgc cacccccacc gacgtctgct ccggaaccca
     6301 ctgagccccc tccgcctaca gaggcgcctg ccatgaccga agagccgacg ccagagccta
     6361 ccgaagaagt tgtcggtgag acctacaaga taggctttgc acctgcaatc acaggccctg
     6421 ggtcaagctt gggggctcct gaacatgaaa caggtcaaat gatcgccgag caactcaacg
     6481 ctcaaggtgg tgtggtcggc ccggatgggg tgaggcatcc cgtggagatc gtcatcttcg
     6541 acaccgagac caacccagat gtggctgtca gtgtagttcg ccgcatgatc gaggaagaga
     6601 acgtgcaagt cgtcgtggcg ggcaccacca gcggaatcag catggccatc gtgccaatta
     6661 ttactgaagc cgaggttccg tatctatcga tggcatcgtc aggtgctatt gtgaaggacc
     6721 cagagaccgg tgaggtgcgt aaatgggctt tcaagacagc ccaagacaac ctggcttcag
     6781 cacaatggca ggccttgtac ctcaataaac tgggatacac gaaagtctgc cacctgtctg
     6841 aaaacagcgg ttatggcgga gacaccctgg cgcaggctca aaaagctttt gagccggcgg
     6901 ggatcgaaat cgtgtactca gactctttcg agcgcacggg gaccgagttc ccacaggtga
     6961 tcaacgttca aaccagcgac tgccaggcag tggtggtggg ggcgatcccg cccgcttcat
     7021 cgaacatctc catcgccctg cgggatgcgc taccagatat gcccatcatc cacggtcacg
     7081 gaacctgcaa ccaagatcat atcgatttga tgggtgcaga agtggcagag ggcatggtca
     7141 gtccctgcgg tagagtcatg gttagcgaga cgctacccga tgacgacccg cagaaagatg
     7201 tcctggtgca atacgttaca gactacaccg cctatacggg caaacccgtc aacaccttcg
     7261 gcggtcatgg ctgggacgcg gtcatgctgg ctgtaacagc agtgtcctca ctgcccgatg
     7321 ggctcgatct agccgcccaa cgtgctgccg tgcgtgattt tctcgagacc ggtgttaaag
     7381 actggcctgg aatcagcggc gtgtacaacc tctcgcctga ggaccacttc agtcttggct
     7441 tcgaaagtct aaccttcgta aaggtcgaag gcggcaaatg gatatacttc cctgaggaag
     7501 agtggtagta acacctcaat tcatacctgg aggccgtaga atctacggcc tccaggaagg
     7561 aaaaaactag atggaacaac aatcgcgccg caagctagtc cagacaatta tctttcttgg
     7621 catcttcctg gcgttgatac ttcttacggt gtttttggga gggcgtaaga atttcggctt
     7681 acaacagtac gcgcaattaa ccattgatgg attgcgaggc ggcgcgatct atgcactgat
     7741 cgccatgggt ttcgtcatgg tcttcaatgt gaccggcatc atcaactttg cccaaggagg
     7801 cttcgtcatg ctgggggcga tgctggcagt gaccatctac gaatgggaac tcccgttacc
     7861 ggcctggatg catttgctta ttagcgtttt gctggcaatt ttgttgacta gcttgattgg
     7921 aatactggtc gaaaggctgt ctatccaccc agcacgctct tcatctactc tcaccctcat
     7981 catcatcacg gtgggtattt acatcgccac ccaaggtttg gctttactga cctggggggc
     8041 agatgcttat atctaccctg cctttacgac tctagagatg gctgatcgca ctatcaggtg
     8101 gatgggcatc gtgatcaagt cacaaagttt ttggatttgg gggattacgg cactagtgct
     8161 gatcgcttta gcgttctttt tcgagcgcac cttgatcgga aaagccatgc gcgcctgtgc
     8221 cgtcaaccgg cggggcgcac gcctggtggg tatcagcacc agcaggatgt ccgtagtagc
     8281 ctttgcgctg gcagccgcac tgggagccat cggaggcatc gtggttgctc ccattttgcg
     8341 cccctcttac gacatgggtc tctccctggg gctcaagggc ttcgtcgctg caatactggg
     8401 aggcttggtg agcacgccgg gggcagtttt gggtggcctc atgcttggtt taatagaaaa
     8461 tcttgctgct ggggtaacca aagccggcat gaaggacatc tacgcattta tcctattaat
     8521 tttagcctta ctcttccgtc cgcaaggatt gcttggtgta cgcaagacgg cggttgaagc
     8581 agatggagaa gatcattaat ggaacctacc gctaaatctg cgccaaacca aaatctactc
     8641 ggatggctgc tgctgaccgt cgtggtgggt gtcttgggtt gggtggagct cgcttctcct
     8701 ttcgggctag acttctccga tatcacccgg ggtatggtca ccctagatac gatgatcaga
     8761 gtggcgattc tgaccatcgt attggtaggc ttgaacctgt tgatgggata cgcgggccag
     8821 gtctcgcttg ggcacgctgc gttctatgga atgggtgcat acgccagcgc catcttcacc
     8881 gcacgggcgg gaagtttatt tggagcgccc gagagcttga tcaacagctg gtggtggccc
     8941 tggttggtga tggtgctcgg gatgattttc gccggcatcc tggcctactt gattgggcgt
     9001 cctattctcc gcctgcgggg tcactatctg gcaatggcta ccctgggctt gggggtgatg
     9061 atctatatct tattccggga gaacatgggg ttccaaggcc aacacatcac gggtacattc
     9121 gacggtattc caggtattcc ccgcctgcgg ctggggagct ttgaaatctg gccgatatca
     9181 cgttactact tctttgtctg gttggtagcc ctgctcgtga ttgcctttgg tctcaacctg
     9241 atcaactcgc gcaccgggcg ggcactgcgg gcaattcacg ggagtgaggt cgccgcgcaa
     9301 gcaatgggtg tagatgtgag tgcctacaaa gcgcgcattt tcgccttgag cgcaattctg
     9361 gccagcctgg cgggcagcct gtatgcacat tttcaagcct cagtttctcc ggcacccttc
     9421 agtttcatcg gctcgctgga attggtcgtc atgtccgcag tcggcggcct ctccagcatc
     9481 tggggcgcac cgttcggtgt ggcagtcatt ctgatcacaa aagaaatctt acgcagccaa
     9541 ctgcggcttt tgatggaggg agccagcgga gagcacgaaa ttatattcta tggtctgatc
     9601 ctggtcgtga tcatgatctt catgcccgaa ggcgtcactg tgggcactgt gcgccgctgg
     9661 cgtgcatggc gcaatcgcag ctacgaaaaa caagctcagg ccgcgcaatc ataggttgat
     9721 ttatttatgg aaaaaaagcg aaccaatacg aacctgcttt caatcaaaga attgagaaaa
     9781 tggtttgggg ggctgatggc agtcaaccgg atatcttttg atgtcaaaca cggtgaaatt
     9841 gtcgcactga tcgggccgaa tggcgcgggc aagaccacta tatttaacct gatcaccggt
     9901 tttcatccga tcgacgatgg agaaattcgg ttcaatgaga agcgcatcga tggcttaaac
     9961 ccccacaaga tcgttcaaga gggcattgtg cgcaccttcc aaaaccttca ggttttcaat
    10021 catatgtcgg tgcttgaaaa tgtaaaagta ggcttgcatc ttcaggggca ttccggaatg
    10081 atcacatcag ctttgcgcat gccttcagcg cggcaggaag agcgtgaatt tcatcaaaaa
    10141 gcactccatt atctggagca ggtcggtctg gttgagcgcg caaacgatct ggctcgcaac
    10201 ctgccttttg gacagcaacg tctgttggag atcgcccgcg ccctggcggt ccagccccaa
    10261 ttacttctat tggacgagcc cgccgccggg ttgaccgtcc cggagacaaa caacctggat
    10321 gagctcattt gccgactcca ggaaaatggg ctgaccatcc tgttggttga gcatgatatg
    10381 ggtctggtaa tggggatcgc cgaccgggtg gtcgtgctgc attacggcac caa
//
LOCUS       DBNHOH010000750         1587 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_302925, whole genome shotgun sequence.
ACCESSION   DBNHOH010000750 DBNHOH010000000
VERSION     DBNHOH010000750.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_302925"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..740)
                     /locus_tag="AC16V7_13505"
     CDS             complement(33..740)
                     /locus_tag="AC16V7_13505"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR6258185.1"
                     /translation="MPTPESARSWYPASDPVHGFDHVLRVYHLAEQLAQAEGADLEIV
                     RAAALLHDVTGDQRSVDSHGLPYAQAGGQLSEISNQHSTINNIRLNHHHASSGFAAQV
                     LRAEGWDEARIVAVQHCIRAHRFRDNRESPHTLEAKILFDADKLDAIGAVGVARAIGY
                     AIQAGQPAYAKPSQQFLDSGELEPGEPHSAYHEFLFKLRHLKDRLYTPAAKSIANERH
                     RVMAEFFERLAGEMGDS"
     gene            complement(821..>1587)
                     /locus_tag="AC16V7_13510"
     CDS             complement(821..>1587)
                     /locus_tag="AC16V7_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6258186.1"
                     /translation="LEVEGPLELAFTFMGAHAIAPEYKENPDDYTDLICQTMLPMLRE
                     WWPHHAPQHHLPFVDVFCETGAFSLDQSRRILETARELGFPLKIHADEFDNLGGARLA
                     AELGAVSADHLVATSPEDIAALAASDTVAVALPCTPFGLSESHYTPAKQILEAGGLLA
                     IATDLNPGTAWCESMQFAIALACRYMRLTPAQAIAAATINAAAAIDRQERIGSIEPGK
                     QADVLILKVPDYRHLGYRFGTNLVETVIKKGHIYHV"
BASE COUNT          327 a          451 c          492 g          317 t
ORIGIN      
        1 atagcattag taaagggaga atcgattttg cactagctat cccccatctc ccctgccagc
       61 cgctcgaaaa attccgccat gacccggtgg cgctcattcg cgatggactt cgccgcaggc
      121 gtatacagcc ggtctttcag atggcgtaac ttgaagagaa actcgtggta agcgctgtgc
      181 ggctcgccag gctccagttc cccgctatcc aggaactgct gggatggctt ggcgtaggca
      241 ggctgaccgg cttggatggc gtaaccaatg gcgcgcgcca cacccaccgc gccgatggcg
      301 tccagcttat cagcgtcgaa aaggatcttg gcttctaggg tgtgtggcga ctcacgattg
      361 tcgcggaagc ggtgagcgcg gatgcagtgt tgcacggcga cgatacgcgc ttcatcccag
      421 ccttccgccc gtaacacctg cgcggcgaag ccggatgaag cgtggtgatg attcaatctt
      481 atattgttga ttgtggaatg ttgattgctg atttctgaca gctgaccgcc tgcctgggcg
      541 tacggcaggc cgtggctgtc cactgaccgc tgatctcctg tcacatcatg tagcagcgcg
      601 gcggcgcgca cgatctccag gtcggcgcct tcggcctgag caagttgctc cgccaggtgg
      661 tagacgcgca gcacgtgatc gaacccgtgc acggggtcgg aggcggggta ccaggagcgg
      721 gcagattcag gagtaggcat agaactaacc cccccacccc tgacccctcc cccaataatg
      781 ggggagggcg gatgggggta atatttcagg gcggctcaaa tcagacatga tagatatgcc
      841 ccttctttat cacggtctct accaggttgg tgccaaaacg ataacccagg tggcggtaat
      901 cggggacttt caagatcaat acatcggctt gcttaccagg ttcaatcgag ccgatgcgct
      961 cttgccggtc gatggcagcc gcggcgttga tggtcgccgc ggcgatagcc tgtgccggtg
     1021 tcaggcgcat ataccggcag gccagcgcaa tggcaaactg catgctctca caccaggctg
     1081 tgccggggtt taagtcggtg gcgatagcca gcaagccacc tgcttccaga atctgtttgg
     1141 cgggggtgta gtgagactcg gacaatccga atggcgtgca aggcagcgcc acggcgaccg
     1201 tatcgctggc agccagggca gcgatatcct ctggagaggt agccacgagg tgatcggctg
     1261 agacagcgcc cagctcggca gccaggcgtg ccccgcccag attgtcgaac tcgtcggcgt
     1321 ggatcttgag cgggaaaccc agctcgcgag cggtctccag tatgcgccga gactgatcca
     1381 aggagaaagc accagtttca caaaatacgt ccacgaaagg cagatggtgt tgcggagcgt
     1441 ggtgaggcca ccattcgcgc agcatgggca gcatcgtctg gcagatcaaa tcggtgtaat
     1501 catcggggtt ttctttatac tctggtgcga tagcatgcgc gcccataaag gtgaaggcca
     1561 gttccaacgg cccttccacc tccaagg
//
LOCUS       DBNHOH010000751         1855 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1135798, whole genome shotgun sequence.
ACCESSION   DBNHOH010000751 DBNHOH010000000
VERSION     DBNHOH010000751.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1135798"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="AC16V7_13515"
     CDS             complement(<1..341)
                     /locus_tag="AC16V7_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 39"
                     /protein_id="HIR6258187.1"
                     /translation="MLLISLVFITLWLRLVNLGYSDYQGDEIRALWLPASGQGAMSFL
                     YTQSKGPMQYLVTYLIKVVDPTYANQFFARLPYTLVGILAIYFFYQLVRTRFGSKVAI
                     YAALFMACNGL"
     gene            complement(412..>1855)
                     /locus_tag="AC16V7_13520"
     CDS             complement(412..>1855)
                     /locus_tag="AC16V7_13520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR6258188.1"
                     /translation="LVNLGYSDYQGDEIKAMWRPAPGQSGIDYLYTQKKGPTQFLVSY
                     LVRFIDPNYSNEFLLRLPFTLAGILGIYFFYRLVKMHFGEKIALYSTLLLSINGIFIG
                     LMRIVQYQPFVILFSVLTLYCFSLASQQEGWKFRGITLGVTFWTVALFSHYDAIFIAP
                     FAFYLLYRWYTQNNDLPVRVRLKHLIIPFAISTLLLAIYFVPYLLSLPSNIKDYWTER
                     ITGEGGTDKRARSSLVSFNLYNPLLATYVYGFFGLLSLPKLKKNIPLILWVALPWAVL
                     ELAVFDPGTHIYTYIIPVIILIAFGMEVLEDVFVKTLGAVWGMRSNILWLCLLFGSLA
                     AISHLIFVDHTPEYPYEKRGILFWTIGGADTVKESKLWNFGFPYYRRWEDIREFVSTS
                     ENTGYYATNENVSISVYYVPYTYFVERAGHYIYIHNPQSFKTWDGRDKIQYWRANHDP
                     VKVFEYNGRVVAEVYFMPPGSLEEIKHAGY"
BASE COUNT          518 a          420 c          493 g          424 t
ORIGIN      
        1 aataacccgt tgcaggccat gaataatgct gcatatatgg cgaccttact accgaagcgg
       61 gtgcggacca gttggtagaa aaagtaaatt gccagtatgc ctactaatgt ataaggaagg
      121 cgggcgaaga actggttggc gtaggttgga tccacgactt tgatcagata agttaccaga
      181 tattgcattg ggcctttgct ctgggtgtac aaaaaactca tcgccccttg tccggatgcg
      241 ggcagccaca aggcgcggat ctcatccccc tggtagtcgg aataacccag attgactaag
      301 cgcagccata aggtgataaa gaccagggaa ataagcagca cctttattcc tgtaatctga
      361 cgaaagactt ccgaatactc ccgcgaaagt ggttttagaa atctaaggga cttaatagcc
      421 cgcatgtttg atctcttcca ggctgcctgg gggcataaag taaacctctg ctactacccg
      481 gccattatac tcaaagactt tcacaggatc gtggtttgcg cgccaatatt gaattttgtc
      541 ccggccatcc caggtcttga agctctgtgg gttgtgaatg tagatatagt gcccggctct
      601 ttcaacaaag tacgtatagg gtacataata aacagagata gacacattct cattcgttgc
      661 ataataaccg gtgttttctg acgtactgac aaattcgcgg atatcttccc agcgccggta
      721 gtatgggaag ccgaaattcc ataacttgct ctcttttacc gtatctgcac cacctatggt
      781 ccagaacaag atgccgcgtt tctcataggg gtattccggt gtgtgatcga caaagatgag
      841 gtgagagata gctgccaggg agccgaaaag caggcacagc cacaagatat ttgaccgcat
      901 cccccagact gcacccaaag tcttgacaaa aacatcctct agaacctcca tgccaaaggc
      961 aattaggatg atgacaggta tgatataggt atagatatgt gtgcctgggt cgaagacagc
     1021 cagctccaaa acagcccagg gaagggcaac ccacaagatg agggggatat ttttcttaag
     1081 tttcgggagc gataaaagcc cgaagaatcc atagacatat gttgccagga gtgggttgta
     1141 cagattgaag ctgaccagcg agctgcgtgc acgcttgtcg gttccgcctt caccggtaat
     1201 cctctcggtc cagtaatctt tgatattgct cggaagggag agcaagtatg gaacgaagta
     1261 aatcgctagc agcagggtgc tgatggcgaa ggggatgatc aaatgcttca gtcgcactcg
     1321 gacaggcaag tcgttgtttt gagtgtacca gcggtagagt aaatagaacg caaaaggagc
     1381 gatgaagatt gcgtcgtaat gcgagaatag ggcaaccgtc cagaaggtca ccccgagcgt
     1441 tatcccccga aatttccagc cttcttgctg ggatgccagc gagaagcagt atagcgtaag
     1501 cactgaaaat agaatcacga agggctggta ctggacaatg cgcatcaggc caataaagat
     1561 gccattgatc gagagcagca gggtggaata cagggcgatc ttctcgccga agtgcatctt
     1621 gaccaggcga tagaagaaat agatacctag aataccagcc agggtgaagg gcaggcgcag
     1681 caggaattca ttggaataat tggggtcgat gaacctgacc aggtagctaa ccaggaattg
     1741 agttgggcct ttcttttggg tgtataggta atcgattccg ctctgtcccg gggcggggcg
     1801 ccacatagct ttgatctcat caccctggta atcggaatag cccaggttga ccagc
//
LOCUS       DBNHOH010000752         1646 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_495458, whole genome shotgun sequence.
ACCESSION   DBNHOH010000752 DBNHOH010000000
VERSION     DBNHOH010000752.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_495458"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="AC16V7_13525"
     CDS             complement(<1..192)
                     /locus_tag="AC16V7_13525"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HIR6258189.1"
                     /translation="MMSDLARSPIGIFDSGVGGLSVLNAIRQQRPSEALLYLADQAHV
                     PYGPRSLEEVRSFSAAITHY"
     gene            complement(182..907)
                     /locus_tag="AC16V7_13530"
     CDS             complement(182..907)
                     /locus_tag="AC16V7_13530"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019342.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIR6258190.1"
                     /translation="MSTALIGVTSSRNSNIYGYPIFTLAEAYTDALLKAGAVPVSIPL
                     GLPNETLLHLLSRLDGVLFSGGGDIDPARYGSAHHPQVNYVDKDRDRVEVSLVDQALE
                     RGLPILGICRGLQVINVALGGTLYEDLGEQLPGSIQHNNFKGRPRDHLAHTVEVEPGS
                     ALSTVMGSTSEQVNSLHHQGIRQLAPSLHPSAWAPDGVIEAVELPGYPFLLAVQWHPE
                     WLQAYPPMRALFKAFIDAASHDV"
     gene            complement(962..1312)
                     /gene="rplS"
                     /locus_tag="AC16V7_13535"
     CDS             complement(962..1312)
                     /gene="rplS"
                     /locus_tag="AC16V7_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIR6258191.1"
                     /translation="MDELIRSVESPQNENVPQLRPGDMVKVFIRIKEGNNERIQEFKG
                     TVIRLRNSGNNANFTVRRVASNGVGVERTFLIRSPRLEKVVVERHSHVRRAQLYYLRE
                     RSGKSSRLKQKFTA"
     gene            1503..1575
                     /locus_tag="AC16V7_13540"
     tRNA            1503..1575
                     /locus_tag="AC16V7_13540"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1537..1539,aa:Glu,seq:ttc)
BASE COUNT          386 a          451 c          451 g          358 t
ORIGIN      
        1 gtaatgggtg atcgcagcag agaatgagcg cacttcttcc agggagcgcg ggccgtaggg
       61 cacatgggcc tggtcggcca aataaagcaa cgcttcgctc ggccgttgtt ggcgaatagc
      121 gtttaacact gagagaccgc caacgccgga gtcgaagata ccaatcggag aacgggcgag
      181 atcagacatc atgcgatgcc gcgtcgataa atgctttgaa cagagcacgc atgggcgggt
      241 aggcttgcaa ccattctggg tgccattgta ctgccaggag gaaagggtaa ccgggcagct
      301 caaccgcttc gatgacacca tcgggggccc aggcacttgg atgcaggctg ggggcaagct
      361 ggcgaattcc ctggtggtgc aagctgttga cctgctcgga ggtgctgccc atgactgtgg
      421 ataaggcgct tccgggctcg acctcgaccg tgtgagccaa atgatcgcgc ggccgacctt
      481 tgaaattatt gtgctggatc gatccgggta gctgttcgcc caaatcttcg tacagcgtgc
      541 ctcccagggc cacgttgatc acttgcaagc cgcgacagat acccaaaatg ggtaatccac
      601 gctctaaggc ctgatcgacc agggacactt ccacgcgatc ccggtcttta tccacataat
      661 tgacctgggg atgatgcgcg ctaccatagc gcgccggatc gatatcgccc ccaccgctga
      721 aaagcacacc atccagccgg gaaagcagat gtaatagcgt ctcattaggc aggcccagtg
      781 ggattgagac cggcaccgcc cctgccttga gcaaagcatc cgtatatgct tcagccaggg
      841 tgaaaatagg atagccgtat atatttgaat tgcgtgagct ggttacgcca atgagagcgg
      901 tggacaaggt ctaaagtaaa aggcgcagcc atatcgctgc gcctctttcc ttctaataac
      961 tctaggcggt gaatttttgc ttcaggcggg aactcttgcc cgagcgctcg cgtaaatagt
     1021 aaagttgggc gcgacgaaca tgcgaatgcc gctcaaccac aaccttctcc aagcgaggcg
     1081 agcgtatcag gaaagtgcgc tccacgccca caccgttact ggcaacgcgg cgcacggtga
     1141 agttggcgtt gttgcccgaa ttgcgcaggc ggatcaccgt acccttgaac tcctggatac
     1201 gctcattatt accttctttg atgcggataa aaaccttaac catgtctccg gggcggagct
     1261 ggggaacgtt ttcattctgg ggtgactcta cggatcttat taactcatcc atcttaacaa
     1321 tccttacttt gcgagtataa cacacaaatc aaagccgcct gaggcggcgc ggaaaggaat
     1381 tctaccatgc acttctctat ttgaaaagac tttttcaaga agtgaattca ttttcgcatt
     1441 tgcatcgatc gagtcgcgta accttcacgc aatccccagc ttatggtaaa atcccgtcgc
     1501 tcgcccccat cgtctagggg accaggacgc agccctttca aggctaaaac cggggttcga
     1561 atccccgtgg gggcacgcga gacagcctct gatcattcag gggctgtttg cttttctaag
     1621 ccctttcata cccgcggggg tatcca
//
LOCUS       DBNHOH010000753         5793 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1648969, whole genome shotgun sequence.
ACCESSION   DBNHOH010000753 DBNHOH010000000
VERSION     DBNHOH010000753.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5793)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5793)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5793
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1648969"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="AC16V7_13545"
     CDS             <1..1192
                     /locus_tag="AC16V7_13545"
                     /inference="COORDINATES: protein motif:HMM:NF046269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HIR6258192.1"
                     /translation="EASRQRWEEWFAAVPPVLEEYEHQYYYAWWIMRVGLLSTRYYTT
                     REVMTPSKSYYIGSWQWDNFFHALAYRYIDTRLAQDQLRILMDHQREDGMIPDAIHDE
                     GTVTHLDFPVEADVTKPPLVAWAAWKLYEHDHDREFLNEIYGPIIKWNNWWFEKNDLD
                     QNGLCEYQHPFSSGLDDSPLWDEGLPVDSPDLNTYLFLQQEALAKIAVEIGEQEEGLM
                     WSQRADAMAQRLIKLRWDKRAGVFWATHLGKRVKVLTPFNLFPLITGKMPKEITDRLV
                     GHLTNPDEFWARYPVPSVALNDAKFDPWQMWRGPTWANINFLLIEGLFRCGYDDLARE
                     LRRCTLDLIASLPDIYEYYHPISGENPPKAAPIYGWSAALFIEMVIQATNEAKGADGE
                     DYEG"
     gene            1182..2411
                     /locus_tag="AC16V7_13550"
     CDS             1182..2411
                     /locus_tag="AC16V7_13550"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6258193.1"
                     /translation="MKVDLLHYSSPPIVGGVESVMAHHARLMADAGHEVRILAGRGDV
                     FDERVQFVNIPFVDSRHTNILEIKRDLDQGNIPPQFPILKDQLVSTLKEITLDTDVLI
                     AHNVCSLSKNLALTAALYQLSQERNSPRLILWHHDLAWTTPRYRSELHDGYPWDLLRR
                     DWAGAKQVVVSALRRQELAELLGIPLERVVVVPNGLDIERFLKLEERSVELVSQLDLL
                     GCAPLLLLPVRITPRKNIELALGVLAALRIQFPQAALVVTGPLGPHNPANIHYFNRLL
                     SLRESLGLKGCAHFLAEVSSEYLPDGVIADFYRLADALFFPSREEGFGIPILEAGMTG
                     LPIFCADIPPLRELAGENALYYDPDSAPEVVASMISDHFASNPIYDLRVRVKKHYTWQ
                     QLYVEQIEPLLTEVVSL"
     gene            2507..4318
                     /locus_tag="AC16V7_13555"
     CDS             2507..4318
                     /locus_tag="AC16V7_13555"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HIR6258194.1"
                     /translation="MRKYTAPAFRKAMVPIIAGCESKDAIRAAGAVSGLGEVVMLGLV
                     AAAPDEPLSASAEQARQVRKMMREFAKKDRFRSLGRVRVSNSPSEELFEAVIDEEPDL
                     LLIEWPRQHEALGISLNEAMQRCTCAVALVRGKIPEKPHHILIPIRGGPFAELSLRIV
                     LAISRNTRASVTALHIIPPSSNYIQEAPFKGLSHVLSNLPEVKYHEIHTDDPVQAILE
                     ASHGFDLVVLGATAQVSPRQEFLGSVAERILNECSAGVMVVKTPRSMPVDMGSELIGQ
                     TAISVLVDKWFAENTYHADEFSNLKELLALKRKQGVTISLALPALNEEETVGNVIQTV
                     KSVLMDEYPILDEIVLMDSNSTDQTREIAKDLGVPVYIHQEILPQYGARRGKGEALWK
                     SLYTTRGDIVVWIDTDIVNIHPHFVFGLIGPLLANPKLQFVKGFYRRPIKVGNRIQAG
                     GGGRVTELTARPLLNLFYPELSGLIQPLSGEYGGRRKTLERLPFSSGYGVEIGLLIDV
                     LENFGLDTIAQVDLRKRVHHNQPLESLSKMSFTIIQTVIRKVEWRYGRNILEEVNKTM
                     KLIRYQSKRFFLEVEEIVERERPPMVDVPEYRELHSL"
     gene            4315..4872
                     /locus_tag="AC16V7_13560"
     CDS             4315..4872
                     /locus_tag="AC16V7_13560"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR6258195.1"
                     /translation="MTHLIFIRHGETDWNTEGRWQGQIDVALNPKGRQQAQDLAEKLS
                     GANLQAIYSSDLQRAIDTARPLAEMTGLTIRTDARLREIHQGEWQGMLVSDIRARYAE
                     LFQQRVNDPLTIAPPGGETVVQVQERVLRALNEIVQRHLNERVAIFAHGFVIAVLRVN
                     LEKRPIDSVWQLVPKSGEIIEYSNT"
     gene            5017..>5793
                     /locus_tag="AC16V7_13565"
     CDS             5017..>5793
                     /locus_tag="AC16V7_13565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6258196.1"
                     /translation="MNSLGPLNDGGRVIIIGGGPSGVACALALQKLGNESGRHLSITI
                     VESKEFSGERHYNTCSGVVSPPLAELLENGLGLPFPYHLARSRISGYVLHTNRAEIQL
                     DDPDRSCIALRRIQFDAYMLDAAQQRGITVEPARAMDMEFHDDRVIVYTDGVPVEGDV
                     VVGAFGMDEGTAALLARHTAYRSPWALSAVVTKYHPGLEAMTEFGPYIHAFLPGDARI
                     EFGAVTPKGNHLTINIGGLSVDSDIMASFLNYPAVSGVLKG"
BASE COUNT         1422 a         1339 c         1565 g         1467 t
ORIGIN      
        1 tgaagcttca agacagcgat gggaggaatg gtttgcagcc gtcccaccgg tcctggaaga
       61 atatgagcat caatactact acgcctggtg gatcatgcgg gtggggctgc tttccacgcg
      121 ttattacacc acccgtgaag tgatgacgcc gtctaaaagc tattatattg gctcatggca
      181 atgggacaac ttcttccatg cattagcata ccgctatatc gacacgcgcc ttgcccagga
      241 tcaactgcgt atcttgatgg accaccaacg agaggatgga atgatccccg atgccatcca
      301 cgacgagggc actgtaacgc acctggattt tccagtggag gccgatgtca caaaaccccc
      361 tttggtggcg tgggcagcct ggaagttata tgaacatgac catgatcgtg aattcctaaa
      421 tgaaatttat ggaccgataa taaaatggaa taattggtgg ttcgagaaaa acgatctgga
      481 tcagaatggt ttgtgtgaat accagcatcc cttctcttca ggcttagatg acagcccgct
      541 ctgggatgag ggtctgccag tcgattcgcc ggacctcaat acctatctgt tccttcagca
      601 ggaagcgctg gcgaagatcg ctgtggagat tggggagcag gaagaagggc tgatgtggtc
      661 tcagcgcgct gatgccatgg cgcaacgctt gataaagctg cgctgggata aacgggcagg
      721 cgttttttgg gcgacacatt tgggaaaacg cgtgaaagtg ttgaccccct tcaatctctt
      781 cccactgatc acgggaaaaa tgcctaaaga gattaccgac cgccttgtcg ggcatctcac
      841 caacccagac gaattttggg cgcgctatcc tgttcctagc gttgcgttga atgatgcgaa
      901 gttcgatccg tggcagatgt ggcgcgggcc tacctgggct aatatcaatt tcttgctgat
      961 tgaagggtta tttcgctgtg gctacgatga cctagcgcgg gagttacgcc gttgtacgct
     1021 ggatttgatt gcaagtttgc cggatatcta cgaatattat catccgatta gcggtgaaaa
     1081 ccctccgaag gctgccccta tctacggatg gtctgccgca ttattcatcg agatggtgat
     1141 ccaagccacg aatgaggcca agggtgctga tggtgaggat tatgaaggtt gatttactgc
     1201 attactcttc acctccgatt gtaggaggtg ttgagagtgt catggcccat catgccaggt
     1261 tgatggcaga tgctggtcat gaagtgcgaa ttctggcggg gagaggagac gttttcgatg
     1321 agcgtgtcca attcgtcaat atcccattcg tcgattcgcg ccatacgaat atattagaga
     1381 tcaagcgaga tttggatcag ggcaatattc cgccccaatt ccctatcttg aaagatcagc
     1441 ttgtctccac attgaaggag atcaccctag atacagatgt gctaattgcc cacaacgttt
     1501 gctcacttag taaaaatctt gccctgaccg ccgcgttgta tcaattgagc caggagcgca
     1561 attcgccgcg gttgattctc tggcatcatg accttgcctg gacaacgccg cgctaccgtt
     1621 cggaactgca cgatggttat ccgtgggact tgctgcgccg cgattgggcc ggtgccaagc
     1681 aggtggtagt ttctgcgttg cgccggcaag agctcgctga gctattaggg attcccctcg
     1741 agcgggtggt ggttgtccca aatggtttgg acattgagcg ctttttgaaa ctcgaggagc
     1801 ggagcgttga actggtgtca caactagatt tgttggggtg cgctccacta cttctgctgc
     1861 cagtcaggat aaccccgcgc aagaatatcg aattagcttt gggtgttttg gctgccctac
     1921 ggatccaatt ccctcaggcg gcgctggtgg tcactggacc tttggggcca cacaatccag
     1981 ctaacattca ctatttcaat cggcttctct cactgcgtga gtcactaggg ttaaagggat
     2041 gtgcgcattt cctggccgaa gttagctcgg aatatttgcc ggatggtgtg atcgccgatt
     2101 tttaccgttt ggccgacgcg ctcttcttcc ccagccgaga ggaaggcttt ggtataccta
     2161 tactggaagc tggaatgaca ggcttgccca ttttttgcgc agatattccc ccactgaggg
     2221 aattggcagg cgagaatgcg ctttattatg accctgattc agcccccgag gttgtggctt
     2281 caatgatttc tgatcacttt gcctcaaacc caatatatga tttgcgtgtc agagtaaaga
     2341 aacactacac ctggcaacaa ttatatgttg aacagattga acctttattg acagaagttg
     2401 tatcgttgta gggttatatg gtgaatgggg ccggcaatac tcaccccaat ctacccagat
     2461 tcctacacta gcgtatgata gaaatatgtt attggattga tgaataatga gaaagtatac
     2521 tgctcctgca ttccgaaaag ctatggtacc tattattgct ggctgtgaaa gtaaagatgc
     2581 aatcagggcg gctggagccg tcagcgggct aggcgaggta gtgatgctgg gtttggtagc
     2641 ggctgcccct gatgagccgc ttagcgcttc agcagagcaa gcccgtcagg tccgcaagat
     2701 gatgcgtgaa tttgcgaaaa aggatcgctt ccgcagcttg ggcagggtgc gagtttcgaa
     2761 ttcaccttct gaggaattgt tcgaagcagt catcgacgaa gaacccgatc tactactcat
     2821 tgaatggcct cgccagcacg aggcactggg gatctccttg aacgaggcaa tgcagcgatg
     2881 tacctgcgct gtggccctgg tacgtggcaa gatccctgag aaacctcacc atattctcat
     2941 acctataagg ggtggtccat tcgccgagct ttcattgcgg attgttttgg cgatctcccg
     3001 aaatacccga gctagtgtta ctgcattaca catcatccct ccttcttcaa attatatcca
     3061 ggaagctcca ttcaagggat tgtctcatgt actatctaac ctgcctgaag tcaagtatca
     3121 tgagattcac acagatgatc cagtgcaggc aatcctagag gcttcgcatg gatttgatct
     3181 agtagtgctc ggcgcaacag cacaggtatc acctaggcag gaatttcttg gctccgtagc
     3241 agaaaggatc ctcaacgaat gttcggctgg tgtaatggtt gtaaaaacgc cgcgctcgat
     3301 gcctgtggat atggggagcg aattaatcgg ccagacagcc atatctgtct tagtagataa
     3361 atggttcgca gagaacacct atcatgccga tgaattctca aacctgaaag agttgctggc
     3421 gctgaagcgc aagcaaggcg taaccatcag cctggcattg ccagccttga atgaagaaga
     3481 gaccgtggga aatgtgattc aaactgtcaa atcggtattg atggacgaat accccatttt
     3541 ggatgaaatt gttttgatgg actccaactc aaccgaccag acccgcgaga tcgccaagga
     3601 cctgggtgtt ccggtatata tccatcagga gatactccct caatatggtg cccggcgcgg
     3661 caaaggagag gcattatgga agagcctcta tacgactcgg ggtgatatcg tggtgtggat
     3721 tgatacggat attgtcaata tccatccgca ttttgtcttt ggtctgatag gacctttatt
     3781 agccaatcca aaactgcaat ttgtcaaagg cttttatcgc cgacccatta aagtaggtaa
     3841 tcgcattcaa gctggaggtg gtggcagggt gactgaactg accgcccgcc ctttgcttaa
     3901 cctgttctac cccgaacttt ccgggttgat ccaaccgctt tctggagagt atggagggag
     3961 gcgcaaaacc ctggaacgtt tgccattctc ctctggttat ggtgtagaga ttggcttgct
     4021 tatcgatgtc ctggagaact ttggattaga caccatcgct caagtggatc tgagaaagcg
     4081 cgtgcatcac aaccagccgc ttgagtcatt gagtaaaatg tcattcacta ttattcagac
     4141 agtgatccgg aaagtggaat ggcgttatgg caggaacatc ctcgaggaag tcaataagac
     4201 catgaagttg atccgttacc agtcaaaacg gtttttcctc gaggttgaag agatcgttga
     4261 gagggaacgt ccccctatgg tagatgtgcc agaatataga gagttacact ccttatgacg
     4321 catctaattt ttatccgaca tggggagaca gattggaata cagaaggtcg ctggcaagga
     4381 caaatcgatg tggctctcaa tccaaaagga cgccagcaag cccaagacct ggctgagaaa
     4441 ctatcgggag ctaatctcca ggcaatatat tccagcgatt tacagcgcgc catagatacg
     4501 gcgcgacccc tggcggaaat gactggatta acgatccgaa cggatgcgcg tttgcgtgag
     4561 attcatcaag gagagtggca gggtatgctg gtatctgata taagggctcg ctatgccgaa
     4621 ttgttccagc agcgagtgaa cgatccacta accatcgcgc cgcctggagg agagactgtt
     4681 gtccaggttc aagaacgcgt gttgcgagca ctgaatgaaa ttgtgcagag acatttgaat
     4741 gagcgcgtgg ctatctttgc acatgggttt gtgatcgctg tgctccgagt gaaccttgaa
     4801 aagaggccca ttgactcggt ctggcaattg gtgccaaaaa gcggggaaat aatagaatat
     4861 tcaaatacat aggtatctat agatccaggt gatgggatgt ttaacaccct aagcaccgtt
     4921 ctcgaactga taactcgatt ttcaggggta aaatacccct gagagattgt ggtctgtgat
     4981 gatgaataat aaagggatct cggtttgcat aatcgaatga attcgttggg cccacttaat
     5041 gatggcggta gggtaatcat tatcggaggt ggcccctcgg gtgtggcctg tgccttagca
     5101 ctgcaaaagc tgggaaacga aagtggacga catctgtcga tcacgattgt ggagagcaag
     5161 gagttttccg gtgaacgcca ctataatacc tgttcgggcg ttgtttcacc tcctctggcg
     5221 gagttgctcg aaaatggact gggcttgcct ttcccctatc atttggcgcg tagccgaata
     5281 tcagggtatg ttctccatac caatcgggcg gagattcaac tggatgatcc ggataggtca
     5341 tgcattgccc tgcgacgcat ccaatttgat gcttatatgc tggatgctgc ccagcaacgt
     5401 ggcatcacgg tcgagcctgc gcgggcaatg gatatggagt tccatgacga ccgggtcatc
     5461 gtctatacag atggcgtccc tgtagaaggt gatgtggtgg tgggtgcctt cgggatggat
     5521 gaaggcactg cagccttgct cgcgcggcac acggcatatc gcagcccctg ggcgctgagc
     5581 gcagtggtga caaagtatca ccccggtctc gaagcaatga cagagtttgg gccatacatc
     5641 catgccttcc ttcctggtga cgcacgcata gaattcgggg cagtgacacc aaaaggtaac
     5701 catttgacaa ttaatattgg aggtctttcg gtagacagtg acataatggc ttcgtttttg
     5761 aattatccgg ctgtgagcgg tgtattgaaa ggt
//
LOCUS       DBNHOH010000754         3998 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1777743, whole genome shotgun sequence.
ACCESSION   DBNHOH010000754 DBNHOH010000000
VERSION     DBNHOH010000754.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1777743"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="AC16V7_13570"
     CDS             complement(<1..210)
                     /locus_tag="AC16V7_13570"
                     /inference="COORDINATES: protein motif:HMM:NF024581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR6258197.1"
                     /translation="MSNQQVELCYHCHKCTAGCPVVGAMAYGPDRILRMAALDQFESI
                     FQSKDIWLCAGCYTCATRCPNSIDIA"
     gene            complement(368..2242)
                     /locus_tag="AC16V7_13575"
     CDS             complement(368..2242)
                     /locus_tag="AC16V7_13575"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6258198.1"
                     /translation="MKIIDVSKPGFRTLVEAELVDSVAWLVRLRWFVGAGVLLGTWSM
                     EALLGLRAPVGPLYAIGAGILGYNLAFYLVERKFTQTSAATIAFRRLSVWQAVLDWLA
                     MIMLIHFSGGIESPMIFFFIFHIIIASIFFTPRTAFGFAILAAALLSGTAILEYYAIL
                     AHKPVVGFLDMPLYQNSLYIAAVLFFFTSTGLFSAYLATSIQKRLRERQEEIIRLSEG
                     LQRATMRLQTLNDGARIINSTLDLPEVLQRLVKNTVDAMGVRACSIRLLNKERQILEP
                     VAVNGLSQAYLNKGIIDAATNPVARRVLGGEVINIPDAPNSTLLQYPEEARQEGIRSI
                     ISAPLLGKSGPLGILRAYAIEPGRFSREDEAFLIAIAAQGSIAIENAMAYKAVEELDL
                     AKSKFIRTVTHELRSPVSVVHSLLRTITTGYAGQVSDQQMDILSRASRRVAYLQKLID
                     DLLDLAVGKADFWKSEDYEPIDIKAVVNKVVKRFEVLSQEKRLSLEFLDETNAGRARV
                     LATQDGMDRIINNLVSNAVKYTPSGGCVTTKLSIAADKVRLIVEDTGIGIPEDAVDHL
                     FEEFYRAPNARNVEREGTGLGLTIARDLVIRFGGSIQVERLQGAGSRFTVTLPVAAES
                     "
     gene            complement(2272..2682)
                     /locus_tag="AC16V7_13580"
     CDS             complement(2272..2682)
                     /locus_tag="AC16V7_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018194949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6258199.1"
                     /translation="MSQKRILLIDDDPDLRLAIRLPLEASGYAVFEASSVVEARTAVK
                     EIKPDLIVLDVMMDTTTAGFQFALELHSTDPQSEFSEFGQVPIIMLTAIHSTTPLRFA
                     PDEDYLPVQTFIEKPIEPDVLLSKVREMLGESVN"
     gene            complement(2679..3152)
                     /locus_tag="AC16V7_13585"
     CDS             complement(2679..3152)
                     /locus_tag="AC16V7_13585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HIR6258200.1"
                     /translation="MNPESGTLVLGLGNPLRRDDGLGGRVIEMLAESKLPAQVVLIDG
                     GTPGWGVTTFLQGWSRVIVIDAADMGSMPGKWDRCQLDAVSLDVGSGHFSLHEPGLSE
                     GLALGQALGTLPQDIVLYCVQPERTEEGIGLTPVVEAVLPELVENILIDLWNKRV"
     gene            complement(3149..>3998)
                     /locus_tag="AC16V7_13590"
     CDS             complement(3149..>3998)
                     /locus_tag="AC16V7_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HIR6258201.1"
                     /translation="PIAMTVGGFTHFPNPDELEDLRYRLEEARRDVDATVDLFASLTF
                     PNFERETEYISLSHPDEYVFIDGEIVSSDGVTLPVEAYRDITNEYLVEHSSAKLARHQ
                     RDSYMVGALARFNNNYAQLHSKAKKAASALGLRPKVINPFLNTAAQVVEIVHCVEGAI
                     AIIDQLLERGIQPEEPAPLESLEGEGISGCEVPRGLLIHHYWIGADGFLSGADCVIPT
                     NQNIASIESDMRALVPSIINNSVNDIQLELEMLVRAYDPCISCSVHVVKIDPLLKVGD
                     AIKNRL"
BASE COUNT         1006 a         1138 c          950 g          904 t
ORIGIN      
        1 ggcaatgtcg atactatttg gacagcgtgt ggcgcaggta tagcaacccg cacacagcca
       61 gatatcctta ctctggaaga tcgactcgaa ctgatccaag gctgccatac gcaggatgcg
      121 atccggacca taagccatcg ctccgaccac cgggcagcca gccgtgcact tatgacagtg
      181 atagcagagc tcaacttgct ggttcgacaa acgcttaaca tcagaaacca ggtgagtaat
      241 cacaggcaca cctatctatt aaccttttta ctaattgaag cgggaaaaaa tactcaattc
      301 tggggttgat tacaccaagc gatcttaata ctcaaacacc acttcgccat gcccccatac
      361 agaggctcta tgattcagca gccactggca aggtcaccgt gaagcggctg cccgcccctt
      421 gaagcctctc aacctgaatc gagccgccaa agcgtatcac caggtctcgc gcgatggtga
      481 gccctagccc tgtaccctcc cgctcaacat tgcgcgcgtt gggagccctg taaaattcct
      541 caaacagatg atccacggca tcttcaggaa ttccgatacc tgtatcctca actatcagcc
      601 gcactttatc agctgcgata gaaagcttcg tcgttacaca gccccctgaa ggggtgtatt
      661 tgacggcatt tgacaccaga ttattaatga tgcgatccat accatcctgc gtcgccaaca
      721 ctctcgccct acctgcgttg gtttcgtcca agaactcaag ggaaagcctt ttctcctggc
      781 ttaatacctc aaaacgtttg accaccttat tcactacagc ctttatgtca ataggttcat
      841 aatcctcgga cttccagaag tccgctttcc ccaccgccag atctagcaaa tcgtcgatca
      901 atttctgcaa ataagccaca cgacggctgg cgcggctgag aatatccatt tgctggtcag
      961 aaacctgccc agcataacca gttgtaatag tgcgcagcag actgtggacg acgctcaccg
     1021 gcgaacgcag ctcgtgggtg acggtgcgaa tgaacttgct cttcgccaga tccaactcct
     1081 caacggcttt ataagccata gcgttttcga tagcgatgct tccctgagca gcgattgcta
     1141 tcaagaaggc ttcgtcctcc cgtgaaaagc gaccaggctc gatcgcatat gccctcagga
     1201 tacccaacgg gccactcttt cccagcagcg gtgcggatat aatcgatcgg atgccctcct
     1261 gccgggcttc ttccgggtat tggagcagcg tgctattagg agcgtcgggg atattaatca
     1321 cctctcctcc caaaacccgg cgggcaactg gattggtagc tgcatcgata atacctttat
     1381 tgagataagc ctggcttaag ccattcacag ccacaggctc cagtatctgt ctctctttat
     1441 tcaacaagcg gatggagcag gcgcgcaccc ccattgcatc caccgtgttc ttcaccaaac
     1501 gctgcaatac ttcggggaga tccagcgttg agttgatgat tctggctcca tcattgagcg
     1561 tttgcaagcg catcgtagcc cgctgaagac cctcgctcag gcggataatc tcttcctgtc
     1621 tttcgcgcaa cctcttttgg atggaggtgg ccaggtaagc ggagaatagt cccgtactgg
     1681 tgaagaagaa caagaccgcg gcgatataaa ggctattctg atataacggc atatccagaa
     1741 agcctacaac cggcttgtgg gcgaggatgg catagtattc tagaattgcg gtccccgaaa
     1801 gcagtgcagc tgccaggatc gcaaatccga aggctgtacg cggcgtaaag aagatcgagg
     1861 cgatgatgat atggaagata aagaagaaga tcatcgggct ctcgatccct ccggagaagt
     1921 ggatcagcat gatcatcgcc aaccaatcca agaccgcctg ccaaaccgat aggcgccgga
     1981 aggcaatcgt tgccgccgag gtttgggtaa attttcgctc caccaggtaa aacgcgagat
     2041 tgtagcctaa aatgccggcc ccaatggcat acaagggccc aacaggagca cgcaacccta
     2101 aaagggcttc catgctccag gttcccaaaa gcacgcccgc gccaacaaac cagcgcaggc
     2161 ggacgagcca ggcaacgcta tcaactagct cagcttccac cagtgtccgg aaacctggtt
     2221 ttgaaacatc tatgatcttc atatttgacc caggggaagt gaaactttcc atcagtttac
     2281 tgattcgcca agcatctcgc gcactttcga tagcaacaca tccggctcga tgggcttctc
     2341 gatgaaggtt tggacgggaa ggtaatcctc atccggagca aagcgcagcg gtgttgtgct
     2401 gtggatggca gtcagcatga tgataggcac ttgaccgaat tcactgaatt ctgattgcgg
     2461 gtcggtgctg tggagctcca gggcaaactg aaagccagct gttgtcgtat ccatcatcac
     2521 gtccagcacg atcaggtctg gcttaatttc cttaactgcg gtcctggctt cgaccacact
     2581 gctggcttcg aatactgcat acccagaggc ttccaaaggc aggcggattg ccagccgcag
     2641 atctggatca tcatcgatca gtaggattct cttttggctc atactcgttt attccataga
     2701 tcaataagta tattttcgac cagttcgggc aaaaccgcct caacgacagg tgtgaggccg
     2761 atgccctctt cggtgcgctc aggctgcacg cagtagagga cgatatcctg cgggagcgtc
     2821 ccaagggctt ggcccaaagc cagaccctct gacaaccctg gttcgtggag ggagaaatgt
     2881 cccgaaccca catccaaact cacagcatcc aattggcagc gatcccactt cccgggcata
     2941 gaccccatgt cagccgcgtc gatgacgatc acccgcgacc aaccctgtag aaaggttgtc
     3001 actccccatc cgggggtgcc accatcgatt agcacaacct gggcaggcag tttactctca
     3061 gctagcatct ctatcacgcg cccaccaagg ccatcatcac gacgcagtgg gtttcccaga
     3121 ccaagaacta aggtgccgct ttctggattc ataggcgatt tttaattgca tctcccacct
     3181 tcaagagggg atcaatctta acaacatgca cggaacacga gatacaggga tcataggcgc
     3241 gcaccagcat ctccagctcc aattgaatat cgttcacaga attattaata atactaggga
     3301 caagggcgcg catatcgctt tcaatgctgg cgatattttg gttggtaggg atgacacaat
     3361 cagccccgct caaaaaacca tccgccccaa tccagtaatg gtgaataagc aacccgcggg
     3421 ggacttcaca gccgctaata ccttcgccct cgagcgattc tagcggtgca ggctcctctg
     3481 gctgtatgcc tcgttccaac aactggtcga taatggcgat tgcaccttca acgcagtgta
     3541 cgatctccac cacctgagcc gcggtattca gaaatggatt aatcaccttt ggtctcaatc
     3601 ctaaggcgct agcggctttt ttagccttcg aatgaagttg tgcataattg ttgttgaaac
     3661 gcgccagcgc acctaccatg tacgaatccc gttgatggcg cgctagctta gcgctggaat
     3721 gctccactag gtattcgttg gttatatctc gatacgcctc aactggcagg gttactccat
     3781 cgctgctgac aatctcgcca tcgataaaaa catattcgtc cgggtgtgac agagagatgt
     3841 attctgtctc gcgctcaaaa ttcgggaacg tcagtgatgc aaataaatcc actgtcgcat
     3901 ccacatcccg cctagcttcc tccagccgat aacgcaaatc ttccaactca tccggatttg
     3961 gaaagtgtgt aaacccacct actgtcatcg caatcggg
//
LOCUS       DBNHOH010000755         1590 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_367217, whole genome shotgun sequence.
ACCESSION   DBNHOH010000755 DBNHOH010000000
VERSION     DBNHOH010000755.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_367217"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..1151)
                     /locus_tag="AC16V7_13595"
     CDS             complement(192..1151)
                     /locus_tag="AC16V7_13595"
                     /inference="COORDINATES: protein motif:HMM:NF024772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6258202.1"
                     /translation="MRYFSTKLVIFVILLSMLLSACAPAAAPAEPVTLKIAVLRILDT
                     LPMYVAQQEGLFDAKGITVEFIPAASAAERDQIITAGQADGMINEVVSTLLYNKDQIQ
                     IQIVRFARTATPTSSQYSILAAQQSGITDVQGLKGVEIGISQGTVIEYITDRLLEAEG
                     LAPEDIKIVAVPKIDVRMNLLGTGELKAATLPDPFSSLALQQGAVLVTDDTKHPEYGY
                     STISFRKPVIDEHPEAIKAFLAAIEEATALINADPTKYENVLTEQKLIPAPLLGSYKF
                     PPFPPASVPSQDQWDDVLTWMKSKGLMDKDVSYTDSVNPSFLP"
     gene            complement(1239..>1590)
                     /locus_tag="AC16V7_13600"
     CDS             complement(1239..>1590)
                     /locus_tag="AC16V7_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HIR6258203.1"
                     /translation="GHARKVIFGLWGLALMSLSKAIVVVDEWVDVQNLSQVSWQVLGN
                     VDWSHNIIIAEGPVDHLDHASYQHSFGGKIGIDATAKQLEEGYSRDWPEVVQMSPEVK
                     ARVDEIWSKLKLDR"
BASE COUNT          374 a          396 c          428 g          392 t
ORIGIN      
        1 ggtggtctta ccgcaaccgg aaggaccaag cactgcccac gcctcgcccc gctcggcctt
       61 ccagtcatag tcctggaaga tatgggggcc ttgctgatag gcgtaggtta cagacctcag
      121 ctcgatcatg cttcatttat aaggtaggca gcaggatgag ggttatgatc atgaccctca
      181 tcctgcatct tttaaggcag aaaagatggg tttacagaat cggtatagga aacatcctta
      241 tccattaaac ccttgctttt catccaggtc aacacatcat cccactgatc ctgcgatgga
      301 accgaggctg gtgggaaagg agggaatttg taactaccga gcaggggcgc ggggataagc
      361 ttttgctcgg ttagcacgtt ttcatacttt gtcggatcgg cgttaatcag cgcagtggct
      421 tcttcgatgg ctgccaggaa agccttgatc gcctccgggt gctcatcgat gaccggcttg
      481 cggaaggaga tggtgctgta gccatattct gggtgcttcg tgtcatctgt gaccagcaca
      541 gcgccctgct gcaaagcgag agatgagaag ggatcgggga gcgtggcagc tttcagctcg
      601 ccggttccga gcaggttcat gcgcacatcg atttttggaa cagcgacgat tttgatgtct
      661 tcaggcgcca ggccttctgc ctccagcaag cgatcggtaa tgtattcgat gacagttccc
      721 tgagatatgc cgatctctac accctttaag ccttgcacat cggtgatgcc gctctgctgc
      781 gcagccagga tggagtactg ggatgaggtg ggagtagctg tgcgggcaaa gcgcacaatc
      841 tggatttgaa tctggtcttt attgtagaga agagtcgaga cgacctcgtt gatcatgcca
      901 tctgcctgac cggcggtgat gatctggtcg cgctcggcag cggatgcagc tgggataaat
      961 tctaccgtta taccttttgc atcaaacaag ccttcttgct gcgccacata catgggcagt
     1021 gtatccagga tacgtaatac cgcaattttc agagtcactg gctcagcagg ggcagcagcc
     1081 ggtgcacagg cgcttaatag catgctgagc aggatgacaa aaatgacgag cttggtactg
     1141 aaatatctca tcgatcaaat ttctcctttg attgataggt gtgtaaccag aaaatgggta
     1201 tgcgagaaga aatccaccat cttcgtattg aacggtagtt accggtccaa tttgagttta
     1261 ctccaaatct catcgacgcg cgccttaact tccggcgaca tttgcacaac ttctggccag
     1321 tcgcgcgaat agccttcttc aagttgcttg gcggtggcat cgatgccaat tttgccgccg
     1381 aacgaatgct ggtaagacgc gtggtctaag tgatccacag gaccttcggc gatgattata
     1441 ttatgtgacc aatccacgtt acccaagacc tgccaggaaa cttgtgagag gttctgtaca
     1501 tctacccatt catcgaccac gactatggct ttcgagagag acatcagcgc tagaccccaa
     1561 aggccaaaga tcactttgcg ggcgtgacct
//
LOCUS       DBNHOH010000756         3287 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1585090, whole genome shotgun sequence.
ACCESSION   DBNHOH010000756 DBNHOH010000000
VERSION     DBNHOH010000756.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1585090"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2591)
                     /gene="gyrA"
                     /locus_tag="AC16V7_13605"
     CDS             complement(<1..2591)
                     /gene="gyrA"
                     /locus_tag="AC16V7_13605"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR6258204.1"
                     /translation="MELGLVRQIDIDAEMQQAYLDYAMSVIVARALPDARDGLKPVHR
                     RIMYAMYDMGLRSDSAYKKSARIVGEVLGKYHPHGDMAVYEAMARMAQDFSMRCLLVD
                     GQGNFGSVDGDPPAAMRYTEARLASPAQHMLADIQKNTVDFEPNFDDTLNEPTVLPAA
                     IPNLLVNGATGIAVGMSTSIPPHNLSEVIDALKYLLGNWERLDDIDLEDLMRFIQGPD
                     FPTGGVIIQEGGEETLATAYGSGRGRVMVQARAHLEEMERGRNRIIVTELPYMTNKST
                     LIERIAELARDGKLEGLADLRDESDRQGMRIVIELNKAADPETVLRDLYKYTPMQSTF
                     SIIMLALVEGEPRMLTLKQALRFYLEHRLEVIRRRSQYDLDKARQRAHLLEGLRIALK
                     NLDEIIGLIRKAPDVETARGRLIRRFKLSDLQAQAILDMQLRRLAALERKKIEEEYKE
                     ILAQIKELEGLLRSPKKMRQLCADELQAVKDAYGDRRRTQIVQLEAGREKRSLLTIEN
                     LAPEKTSWLVVTTDGLLSRTLEDKVPRQSGNDAPNWLLRVNTRDTLYLVCEQGETAAV
                     PVLAIPEADKPSEGVIYSKVSALKDENKLAVLFTLPPKGERADDWYVITATRKGMVKK
                     SAINDLPGTSANIFTLVKVNEGDRLGWLQLTNGGKEVLLVTALGMAIRFNEDEVRPMG
                     LVAAGVMGMKLQSKDEVAGMEILPQPGEVFIAVSDGSAKRVAPAQFPKQGRYGQGVVA
                     WKLPGKARVIGLAVGKGTARVTLHLSKLAPKMSRLDEAPLQSRVARGARIQELKPGEQ
                     VLRLTVPWDVERPIAGVKAKVGKRVRRSTAESTSSRSAKPAPRKRGPAKASQAEAKPV
                     KSRSKKPS"
     gene            complement(2757..>3287)
                     /locus_tag="AC16V7_13610"
     CDS             complement(2757..>3287)
                     /locus_tag="AC16V7_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258205.1"
                     /translation="CSNASLFDQAPPPDTRVYMTKDDADSQVPFIWIELPSKLEIDRN
                     DIRVFMARRDPPGMLVPTEVELPCIIQSANDHLELRPVVLPFMYDGHQLVGSYAWLAC
                     ASCVECYMNWETTLEMVATLEEDMMTFSFGVRHMGHNIQEDYLRIELEQASPTAKEPG
                     IQCWSAENCKQMEFAR"
BASE COUNT          715 a          948 c          804 g          820 t
ORIGIN      
        1 gacggtttct tcgatctgct cttgactggt ttagcctcag cctggctggc cttggccggg
       61 cctcttttgc ggggagcagg ctttgcagat ctggacgatg tggactctgc cgtcgatcgt
      121 cgaacccgct tgccaacctt tgctttaact cctgctatgg ggcgctccac atcccagggc
      181 actgtcagtc gtaacacctg ctctccaggc ttaagctcct gtatcctggc accgcgtgcc
      241 accctgctct gcaatggggc ttcatctaac cgcgacatct taggagccag cttgctcaag
      301 tgtagggtga cgcgtgccgt acctttgccc accgccaagc cgatcacgcg cgcttttcca
      361 ggtagtttcc aggcgaccac cccttgccca taccgccctt gcttagggaa ttgagcaggc
      421 gcaacgcgct tggcgctgcc atccgagact gcaataaaaa cttcgcctgg ctgcggcagg
      481 atttccatac ctgctacttc atcttttgac tggagcttca tccccattac cccagccgca
      541 accaacccca tcggtcgcac ctcgtcttca ttaaatcgta tggccatacc taaagctgtc
      601 accaatagca cttccttgcc cccattcgtc agctgcaacc atccaagacg atcgccttca
      661 ttgactttta ccaaagtaaa tatatttgcc gatgtgccag gcaggtcgtt aatcgcagat
      721 ttcttgacca tacccttacg ggtggcagtg atcacgtacc agtcgtcggc gcgctcacct
      781 tttggtggta aggtaaataa aaccgccagc ttgttttcgt ctttcaaggc agaaaccttg
      841 ctgtagatca cgccttcgga gggtttatcc gcctcaggga tagcaaggac aggtactgca
      901 gcagtctccc cttgctcaca gaccaggtaa agcgtatcac gggtattcac acgtagcagc
      961 cagttcggcg catcattgcc gctttggcgg ggaactttgt cctccagtgt acgagagagc
     1021 agcccatcgg tggtcaccac aagccaggaa gtcttctctg gggcaaggtt ttcaatagtc
     1081 aatagagagc gcttctctct tcccgcttct aattgtacga tctgcgtgcg gcgacggtcg
     1141 ccatatgcat ctttgactgc ctgcagctca tctgcgcaaa gctggcgcat ctttttcggc
     1201 gaacgcaata gcccttccaa ctctttgatc tgcgcgagaa tctctttgta ttcctcttcg
     1261 attttcttgc gttccagtgc tgccagacgg cgcaactgca tatcgaggat cgcttgggct
     1321 tgaagatcgc ttaacttgaa tcgtctaatt aaccgccctc gcgctgtctc aacatctggg
     1381 gcttttcgga tcaagccaat gatttcatca agattcttca gggcaatgcg caagccctca
     1441 agcaaatgtg cccgttggcg tgctttatct agatcatact ggctcctacg ccggatgact
     1501 tccaggcggt gttccaggta gaagcgcaaa gcctgtttta aggttagcat gcgcggctca
     1561 ccctctacca gcgccagcat gatgatgctg aaggtagact gcatgggggt gtacttgtac
     1621 aagtcacgta gcactgtctc tggatctgcg gctttgttta attcgatgac gatgcgcatg
     1681 ccttgccgat cagattcatc gcgcagatcg gctagccctt ccagttttcc atcccgcgct
     1741 aattcagcga tgcgctcgat cagcgttgat ttattggtca tatacggcag ttcggtgacg
     1801 atgattcggt tgcgcccgcg ctccatctct tccaaatgcg cccgcgcttg caccatcaca
     1861 cgtcctcgcc ccgagccata ggcggtcgcc agagtctcct cgccgccctc ctggataatg
     1921 acccctccgg tggggaaatc gggcccttgg atgaagcgca tcaggtcttc caggtcgata
     1981 tcatccaaac gctcccagtt tcccagaagg tacttcaagg catcaatcac ttcactcaaa
     2041 ttgtggggtg ggatgctggt ggacatccct acggcgatac ctgtagcccc gttcaccagc
     2101 aggtttggaa tcgcagcggg taacaccgtg ggctcgttaa gggtatcgtc gaaattcggc
     2161 tcgaagtcca ccgtattttt ttgaatatct gccagcatat gttgggcggg cgacgccaat
     2221 cttgcctcgg tgtaacgcat ggcagccggt gggtcgccat ccacagagcc aaagttgccc
     2281 tgcccatcaa ccaggagaca gcgcatggaa aaatcctgcg ccatgcgcgc catggcttca
     2341 tataccgcca tatcgccatg cgggtggtac ttacccaaca cttcacctac gatgcgggct
     2401 gattttttat acgctgagtc tgagcgcagt cccatatcat acatggcata cataatccgg
     2461 cggtgaaccg gctttagacc atcgcgcgca tcgggcaagg cgcgcgctac aatcacgctc
     2521 atggcataat ccaggtaggc ttgctgcatc tcagcgtcga tgtcaatttg gcggaccagc
     2581 cctagttcca taatccgttc tcccgtttca aatttaacgt accaaatgcc atgtgccacg
     2641 cgccgaaaga cataacacat gcaatgtgat acgtgataac ttcgcactcc gcacgtaaag
     2701 ttagaatgtt tgtgcgaagt gtatcttaaa ggataaagag ggaaacgtca agcaggttat
     2761 cttgcgaatt ccatttgctt gcagttttct gcgctccaac attggattcc gggctccttc
     2821 gcagtggggc ttgcctgctc aagctcaatg cgaaggtaat cttcctggat attgtgcccc
     2881 atgtgacgca caccaaatga gaaggtcatc atgtcctctt ccaaagtcgc caccatctcc
     2941 agcgtagtct cccagttcat atagcattca acgcatgacg cgcatgctag ccacgcatag
     3001 cttccgacca gctgatgacc atcatacatg aaaggcaata ccacggggcg cagctcaaga
     3061 tgatcgtttg cagattgaat tatgcaaggc agctcaacct ctgtaggtac tagcatgccg
     3121 ggaggatccc tacgagccat gaacacccgt atgtcattgc gatcgatctc aagcttggag
     3181 ggaagctcga tccaaatgaa cggaacctgg ctatcagcat cgtctttggt catgtagact
     3241 ctcgtatcag gtggaggagc ttgatcgaat aaagaagcat tactgca
//
LOCUS       DBNHOH010000757         6539 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1136081, whole genome shotgun sequence.
ACCESSION   DBNHOH010000757 DBNHOH010000000
VERSION     DBNHOH010000757.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6539)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6539)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6539
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1136081"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="AC16V7_13615"
     CDS             complement(<1..687)
                     /locus_tag="AC16V7_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HIR6258206.1"
                     /translation="MSALFRALRPRQWTKNSLLFAALVFDRQLTLSHLPAILHTIAGF
                     FIFCLLSGAVYIINDIMDREADLNHPQKSLRPIASGELNLTIARTVAIGLIVVLLPIS
                     YMLSPLFAGIALIYFVSNLAYSKWLKHVPILDVLTISLGFVLRVGAGVSLIVVTRFSP
                     WLYVVTTLGSLYIGFGKRRAELALLAEGANSHRKVLDGYTIPLLDQFITIVSGTTIIA
                     YSLYTFSAPNL"
     gene            978..1856
                     /locus_tag="AC16V7_13620"
     CDS             978..1856
                     /locus_tag="AC16V7_13620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR6258207.1"
                     /translation="MTFWKYYHLAKNIPDALHVLTQAPGPARLISGGTDLLLDLQQGR
                     HSPVHTLVDVTHIPEMSIIEIRQDRLFVGAAVSLNSIVASPLVQRHAQALVEAAGLIG
                     GPQVRNTATLGGNVAHALPAGDGTIALLALEALAEVANQRGLRQVPMRELFLGPGRSA
                     LDPQNDMLVGFYLPLRSTGQGSAFKRVMRPQGVAIAILNSAVWVQREGERIAGIRIAM
                     GPAGPTPFRALAAEETLRMQLMDEESVSRAIETLLAEARFRTSPHRATLEYRREMANI
                     LLTDTLTLAWHRTFEK"
     gene            1882..4752
                     /locus_tag="AC16V7_13625"
     CDS             1882..4752
                     /locus_tag="AC16V7_13625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR6258208.1"
                     /translation="MDSLLGEQEEFVMNMVINGQRMTIEALPGEMLSDLLRERLGLIG
                     TKIGCNEAECGACTVLVDDQPVLSCVFPAAKADGKNIFTIEGLASLYSIARSNRSGDG
                     KEEDAQETLHPLQEAFIQHGAVQCGFCIPGQIMTAYALLRAVPEPSEADIRYALKDTL
                     CRCAGYPTIIRAIQTAAYSVRTGEPLAPPPIPGLDEMRPHGVIGTLQPRPDSVEKVTG
                     KAIFTDDLKFEGMLHARVKRAGVPHAIVRRIDVSKARLLPGVVAILTADDIPGEHYHG
                     LVIKDWPSLVGIGERVRYVGDSLALVAAETREIATQALELIEVELEPQPVVSDPVQSR
                     TPQAPSLHPNGNLLKHIKVRKGDVEQGLAEADVILEHTFHTQMTEHAFMEPECSIARP
                     LPDGRMEIYVGSQIPYSDRNDIARALGWPEERVRVVGQLMGGGFGGKEDIAGQIHAAL
                     LVNATGRPVKLLFDRHESLLVHPKRHPTQIHVKVGAKRDGRLTGVETELYGDTGAYAS
                     LGEKVMTRATTHSSGPYEVPHARADCYAMYTNNPPAGAFRGFGVTQSAFAIESMMDML
                     AETLHIEPVELRRLNALRVGSVTNTGQTLRESVGLLECIDRVDAEMRRIGGSDPFAPR
                     RVDGAPQLVRAWGFAAAYKNTGLGGGAPDKAGAEVELFANGTLEVRTSSAELGQGLVT
                     VLQMIVAEEFAISPSKVRVLVMDTDLTPDGGPTTASRQTYVTGNAARGAAQALRQAIT
                     TTLAEKYDQPPERIRFVEGLAQVNGHTLPLGNIVASMRAEGREPRAMYEYWAPATKPL
                     GQGGDMHFAFSFATQAAEVEVNTLTGEVQVLRVIAANDVGKAVNPLGLHGQVEGGVMM
                     GLGNALTEHYIVENGQVFTDHLARYRMPSIMHTPEIVSIVVEHPAADGPYGAKGVGEI
                     VSIPTSPAITNAIYNAVGVRVDRLPVDQAEILKNIRRQKATG"
     gene            4858..5487
                     /locus_tag="AC16V7_13630"
     CDS             4858..5487
                     /locus_tag="AC16V7_13630"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015752407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6258209.1"
                     /translation="MNITKPPRWLEWAREIQALAQTGYHYSENEYQRERYHRLTEIAA
                     EIISEHSELPFDPLLSTFNRQIGYATPRVDVRGAVLTDGKLLLVRERQDGGWTMPGGW
                     ADVGDVPSQAVEREVWEEAGLRVKARKVIGVYDANRVGPLEIFHAFKVVFLCELLGGQ
                     PRPSSETSEVAYFGPDEIPTLLSGERTKPRHISDAFKILMNPDCPTVFD"
     gene            5487..5975
                     /locus_tag="AC16V7_13635"
     CDS             5487..5975
                     /locus_tag="AC16V7_13635"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012982.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine phosphatase family protein"
                     /protein_id="HIR6258210.1"
                     /translation="MTLIEFPLGFSGRVFRSPMPFTAYDPFEMLFKQYQEQEVSVVVL
                     LVEDEQCIARTGRNLRLFYHEKGMEVIHLPIPDFGVPSLPALEEAVQSAIDRANQGKN
                     IVVHCYAGLGRTGTFLACLARRAFGMTGDQAVKWVRSYLSPAIETPGQVRVIQEFGRD
                     LD"
     gene            5975..>6539
                     /locus_tag="AC16V7_13640"
     CDS             5975..>6539
                     /locus_tag="AC16V7_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR6258211.1"
                     /translation="MLIVNGKLITWEVPNQVLEGQALWISEGLIAEVGPQADLIARHP
                     DAERLDARGQYIMPGNICAHTHFYGAYARGMAIPGPAPKDFPEILEKLWWPLDRALRM
                     DDVRASAEVMLVDAIKHGTTTLIDHHASPNAIEGSLDTIAEAVDRSGLRAVLCYEVTD
                     RNGLDGARRGIDENMRFIKEHSSGGSGS"
BASE COUNT         1416 a         1674 c         1990 g         1459 t
ORIGIN      
        1 caaattgggt gctgagaagg tgtacaggct gtaggcgatg atggtggttc cggacacaat
       61 ggtgatgaat tggtctaaga gggggatggt gtagccgtcc aggactttgc ggtgagaatt
      121 tgccccctcc gccagtaagg ccagctcggc gcggcgctta ccgaagccga tatagagtga
      181 cccgagggtg gtgaccacgt acagccaggg cgagaaacgc gttaccacga tcaacgatac
      241 gccagcacca actcgcaaga cgaatcccaa ggagatggtg agaacatcca ggatcgggac
      301 gtgtttgagc cacttggagt atgccaggtt gcttacgaag taaatcaagg caatgcctgc
      361 aaacaaaggc gagagcatgt acgagatcgg gagtaagacg acgatcagtc cgattgctac
      421 cgtgcgggcg atcgttagat tcagctcccc agaagcgatg ggtcgcaggc ttttttgggg
      481 atggttcagg tcggcctccc tgtccatgat gtcattgatg atatagaccg ccccagataa
      541 gagacagaag atgaaaaagc ccgcgatcgt gtgcaaaatg gctggcagat ggcttagggt
      601 gagctggcgg tcaaaaacca gtgcggcaaa aagcaggcta tttttcgtcc actggcgcgg
      661 gcgcagcgcc cggaacaagg cagacatggt cttccaaaac atgggggtat gatagtaggg
      721 attgtgttat tcgtcaacca agaattctgt ttggcgcgag gagcatcgat agcgtattat
      781 cggttcgtgt ggggctgaat ggctgattta tcaaggggac ttattgtttt atggactcaa
      841 ttgctcggtt ttcgtgttaa aataagacct actggatcgg cggtcgctgc agggtttgcc
      901 tgctgcggcg tcgggtcagt gtatcccgtc gaaaagagcc gccgttctga tcttggctgc
      961 aagggaaacg ccgatccatg actttttgga agtattatca cctcgccaaa aacatccccg
     1021 atgcattgca cgttcttacg caggcacctg gtcccgcccg actgatctcg ggtggcactg
     1081 acctgctgtt ggatttacag caggggcgcc actcccccgt acacacactg gttgacgtta
     1141 cgcatatccc tgagatgtcg atcatagaga ttcgtcagga tcggcttttc gtgggagcgg
     1201 ctgtgtctct taatagcatt gtggcatcac ctctcgttca gcgacatgcc caggcgttgg
     1261 ttgaggctgc tggactgatc ggcggaccgc aagtgcgcaa taccgctact ttgggtggga
     1321 atgtggcaca tgcgctgccg gcgggcgatg gcactatcgc cttgctggcc ctggaggcat
     1381 tggcggaggt ggccaatcag cggggcttgc gccaggttcc tatgcgagag ttatttctgg
     1441 gacccggtcg ctcggctctc gatccgcaga atgatatgct ggtcggattt tatttaccgc
     1501 tgagaagcac cggtcaagga tcggcattca agcgggtgat gcggcctcag ggtgtcgcaa
     1561 ttgctatcct gaactcagcg gtctgggtac agcgagaagg ggaacgcatc gccggtatcc
     1621 gcattgcgat gggtcctgct ggccctactc catttcgagc gttagccgct gaagagaccc
     1681 tgcgtatgca acttatggac gaagagtcgg taagccgggc tattgagacc ttgctagctg
     1741 aggcgcgctt ccgcaccagt ccgcaccgcg ccacgttgga gtaccggcgg gagatggcga
     1801 atattttgct gacagacaca cttacgctgg catggcatcg tacctttgaa aagtgaattc
     1861 ggaagataga caacagaaag attggatagt ttattaggtg agcaggaaga gtttgttatg
     1921 aatatggtga tcaatggaca gagaatgacg attgaggcgc tgcctggtga gatgctcagc
     1981 gatttgctgc gcgagcggct tgggttaatc ggcacgaaga ttggttgtaa tgaggcggag
     2041 tgtggagcct gcaccgtcct ggtggacgat cagccggtgc tctcctgtgt tttccctgct
     2101 gccaaagcgg atggcaaaaa tatttttacc atcgaaggtt tggcttcact ttattctatt
     2161 gctcgttcca atcgtagcgg agatggaaaa gaggaggatg ctcaggagac tctccacccc
     2221 ctccaggaag ccttcattca gcatggcgcg gtgcagtgcg gtttctgcat ccctgggcag
     2281 atcatgacag cctacgcctt gttgcgagcc gtgcccgaac ccagcgaggc agacattcgc
     2341 tatgccctga aagatacttt gtgccgctgt gcagggtacc cgacgattat cagagctatc
     2401 cagacagctg cctattctgt gcgtactggg gagccacttg cacccccgcc tatcccgggt
     2461 cttgatgaaa tgcgcccgca tggagtgatt ggcacgctcc agccacgtcc tgactcggtt
     2521 gagaaagtga ctggaaaagc tatattcacc gacgatttaa aatttgaggg tatgctccac
     2581 gcgcgcgtca agcgcgctgg ggtgccccat gcaattgtcc gccgtataga cgtctcgaaa
     2641 gccaggctat tgcccggtgt ggtcgccatc ctgacggcag acgatatccc cggcgagcat
     2701 tatcacggac tggttatcaa ggattggcca agcctggtgg ggataggaga gcgggtgcgt
     2761 tacgtgggag attccctagc tctggtcgct gcagagacgc gtgaaattgc gactcaggca
     2821 ctagaactca tcgaagtaga acttgaaccg cagcctgttg tcagtgatcc tgttcaatcg
     2881 cgcacgccac aagcgccgag tttacatccg aatggcaatc tgctcaaaca tatcaaggtg
     2941 cgcaaaggtg atgtcgaaca gggtttggct gaggccgatg tgatcctgga gcacacgttt
     3001 cacactcaaa tgaccgagca tgccttcatg gagccggagt gcagcatcgc gcgtcctttg
     3061 cctgacgggc gcatggagat ctatgttggc tcacagatac catattcgga tcgcaatgat
     3121 attgcccgcg cgctgggctg gcctgaggag cgggtgcgcg tggtgggcca gttgatggga
     3181 ggaggttttg gcggcaagga ggatattgcc ggtcagatcc acgctgcgct gctggtgaat
     3241 gccacgggtc gcccggtgaa actactattc gaccggcatg aaagcctgct ggtgcaccca
     3301 aaacggcacc ccacgcagat ccatgtcaag gtcggggcga agcgtgatgg gcgcctgaca
     3361 ggggttgaga ctgagcttta tggggatact ggcgcctatg cgtccttggg tgagaaagtc
     3421 atgacccgcg ccaccaccca ttcctctggc ccttacgagg tgccccatgc ccgtgcggat
     3481 tgctacgcca tgtacacaaa taacccccct gccggtgcat tccgcggctt cggtgtgacc
     3541 caatcggcgt tcgcaattga gagcatgatg gatatgcttg ccgagacgct gcacattgag
     3601 ccggttgaac tacgccggct gaacgccctg cgtgtgggca gcgtgaccaa tactggtcag
     3661 accttgcgcg agagcgtagg gctcttagaa tgcatcgacc gcgtggatgc ggagatgcgt
     3721 cgtattggcg ggagcgatcc atttgccccg cgccgcgtgg atggtgcgcc tcaactggtg
     3781 cgtgcttggg gattcgctgc ggcatacaag aacacaggat tggggggtgg cgcgcccgat
     3841 aaggctggcg cagaagttga attgttcgcc aacggcacgc tggaggtgcg tacttcttct
     3901 gctgagcttg gtcaggggct ggtcaccgtg ctgcagatga tcgttgctga ggagttcgct
     3961 atatcacctt cgaaggtgcg tgtgctggtc atggatactg atctgacccc cgacggtggc
     4021 ccgacgaccg cttcccgcca gacttatgtt acagggaatg ccgcccgcgg cgccgcccag
     4081 gcgctccgac aggctatcac caccacccta gcggagaagt atgatcagcc cccagagcgc
     4141 attcgcttcg tcgagggatt agcccaggta aacgggcaca ccctgcctct gggcaatatt
     4201 gtcgccagta tgcgcgccga aggccgcgag ccgcgcgcca tgtacgaata ctgggcaccg
     4261 gctaccaaac cgcttggtca aggcggcgat atgcacttcg ccttttcctt tgcaacccag
     4321 gctgccgaag tggaggtgaa tacgctgact ggcgaagttc aggtgctgcg cgtaattgcc
     4381 gccaacgatg taggcaaggc tgtcaatccg ctgggcttgc acgggcaggt tgaaggaggc
     4441 gtgatgatgg gcttgggcaa cgcgctcact gagcactata tcgttgaaaa tgggcaagtg
     4501 tttaccgacc atctggcgcg ctatcgtatg ccgtccatca tgcacacgcc ggagattgtc
     4561 tccatcgtcg tggagcaccc tgcagcagat ggcccgtatg gagccaaagg ggtcggtgaa
     4621 attgtcagca ttccaacctc accggcgatc acaaacgcca tttacaacgc ggttggcgtg
     4681 cgtgtggacc ggctgcctgt agatcaggca gagattctaa agaacattcg ccggcagaag
     4741 gctaccggtt gatgcctaac ctgtgcagaa tggttctcga tcaccaaatg tcgtggtcgg
     4801 cagttcggac tatctgttaa ggatggggat tgtgtataat ccacttggga ttatttaatg
     4861 aatataacca agccaccgcg ctggcttgaa tgggcgcgtg aaatccaggc tctggcgcaa
     4921 accggatacc actacagcga gaacgaatac cagcgggagc ggtaccaccg tttaaccgag
     4981 atcgccgcgg agattatcag cgagcacagc gagctgcctt tcgatccgct gttatctacc
     5041 tttaaccgcc aaattggcta tgccacacct cgcgtggatg tgcgcggggc ggtattaacc
     5101 gatggcaagc tattactggt gcgtgagcgc caggatggtg gctggacgat gcccggtggc
     5161 tgggcggacg tcggtgatgt gccctcgcag gcggttgaga gagaggtctg ggaggaagcg
     5221 ggtttgcgtg tcaaagcccg caaggtgatc ggtgtgtacg atgccaaccg cgttggcccg
     5281 ctggaaatct tccatgcatt caaggtggtg ttcttgtgcg agttgcttgg aggccaaccg
     5341 cgaccaagca gcgagacaag cgaggtggca tactttggac cagatgaaat tcctactctc
     5401 ctttcaggcg agcgcactaa gcctcgccac atcagcgacg ctttcaaaat cttgatgaat
     5461 ccggattgtc ctacggtttt tgattgatga cattgattga attccccctt ggtttttcag
     5521 gacgcgtctt tcgcagtcct atgcctttca cggcttatga cccgtttgag atgctattta
     5581 aacaatacca ggagcaagaa gtttccgtgg tcgtgcttct agtagaagat gaacaatgca
     5641 tagccaggac tgggcgtaac ttgcgcttgt tctaccatga gaagggcatg gaggtgatcc
     5701 acttgcccat acctgatttt ggcgtcccct cgctgccagc tttagaggag gcggtgcaat
     5761 ctgctatcga tcgcgccaat cagggtaaaa atatcgtggt gcactgttac gccggcctgg
     5821 gacgcacggg cacattcctg gcctgcctgg cccggcgtgc ttttgggatg actggcgatc
     5881 aggcagtgaa gtgggtgcgc tcctacctct cgccagctat tgaaacaccc ggtcaggtcc
     5941 gggtgatcca ggaatttgga agggatttag attaatgtta atcgtaaatg gcaagctaat
     6001 tacctgggaa gtgccaaatc aggttcttga ggggcaagcg ctatggatct ctgagggatt
     6061 gatcgctgaa gtcggtcccc aggcggatct gatcgcacgt catccagatg cagagcgctt
     6121 ggatgcccga gggcagtaca tcatgccagg caatatctgc gcccataccc atttttatgg
     6181 agcctacgcc cgtggcatgg cgataccagg cccagcccct aaagattttc cagaaatcct
     6241 agaaaaacta tggtggccgc tggaccgggc gctcaggatg gatgatgtgc gtgcttcggc
     6301 agaagtgatg ctggtggatg ccattaaaca cggcacgacg acgctcatcg accatcacgc
     6361 ttcgcctaat gccatcgaag gctcgctgga taccatcgcc gaagcggtag atcgctccgg
     6421 tttgcgggcc gtgctatgct atgaggtgac agatcgaaat ggtctggatg gggcgcggcg
     6481 tggcatcgat gaaaatatgc gcttcatcaa ggaacactcc tcgggcggtt caggatcat
//
LOCUS       DBNHOH010000758         2911 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_174164, whole genome shotgun sequence.
ACCESSION   DBNHOH010000758 DBNHOH010000000
VERSION     DBNHOH010000758.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_174164"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="AC16V7_13645"
     CDS             <1..457
                     /locus_tag="AC16V7_13645"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012708373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - L-methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HIR6258212.1"
                     /translation="FRSLRLAKKIEAGAEFIQTQYCFDFRRLCKFMDSVRDMGLHEQA
                     YILVGVGPLRSAKAAEWMRSRVPGVVIPDEIIRRLNGVPSSRQWEEGKRICIEIIQQV
                     REVEGVSGVHVMAYRQEELVAEIIQEAGLLPRRRISELGQYDPCEEYTI"
     gene            461..1369
                     /locus_tag="AC16V7_13650"
     CDS             461..1369
                     /locus_tag="AC16V7_13650"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR6258213.1"
                     /translation="METIITSPQKVVKIGPEHPFVVIGERINPTGRKLLAAEMAAWNF
                     DRVISDAIAQVQAGARMLDVNAGIPLADEPAILAEAVRVVQSVVDVPLSIDSSVIKAL
                     EAGLAAYNGKPLVNSVTAEEDRLEVVLPLVKKYGAAVIGIANDETGISEDPDVRFTIA
                     KKIVERAEDYGIPRQDVLIDPLAMPVGAVRMAGVTLFTLVRRLRDELGVNTVCGASNI
                     SFGLPERGTLNATFLAMAISAGMTCAITNPLEESIRKTVLAADVMMGRDENCLAWLSA
                     HREEAPGESGSARQRRRSRREELSRK"
     gene            1410..2225
                     /locus_tag="AC16V7_13655"
     CDS             1410..2225
                     /locus_tag="AC16V7_13655"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6258214.1"
                     /translation="MAIDKEKIRLAAEQETLLITLYSKVYGCPQTVFKDEKTRQILDR
                     IDYDFSQLKVPTGTRLTVCLRAKKFDEIVREFLASHPQGVVLHLGCGLDTRFVRVGSP
                     RIDWYDLDLPEVIDLRKKFFDETDNYHMIPSSVTDLSWVDRIPTRDQDVLVVAEGLLM
                     YLKDEQVKTLVLRLKEAFPGCELAFDAYSTLTVRRVKDHPSIKKTGALIQWGIDDARQ
                     IEHWASGIRLKEEWFYTQSKDIKKLAFSHRLIFAIAGLFSVARKAHRILYYAL"
     gene            2320..>2911
                     /locus_tag="AC16V7_13660"
     CDS             2320..>2911
                     /locus_tag="AC16V7_13660"
                     /inference="COORDINATES: protein motif:HMM:NF015448.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HIR6258215.1"
                     /translation="MMIISKSWEQAYPGAVVGELRLGDVANPEGHAALEQRKVKLEAD
                     LRARFSGYDRAGLRDIPVLQAYHAYYKRFDKTYHVQLQLESVVLKGKSIPRVAALVEC
                     MFMAELKNLLLTAGHDADRLIDPVQLGVAQGDEVYMLLNGQEQALKAGDMYIADREAA
                     ISSVIYGPDRRTRIRQETKRALFTVYAPPGIGEEAVW"
BASE COUNT          650 a          698 c          917 g          646 t
ORIGIN      
        1 tttccgctcc ctgcggctgg ccaagaaaat cgaggctggg gcggagttta tccagacgca
       61 gtattgtttc gactttcgcc gcttatgcaa atttatggac tctgtacgag atatgggatt
      121 gcacgaacag gcctatatct tggtgggggt aggcccgctg cgctcagcaa aagcggcgga
      181 atggatgcgt agccgcgtac caggtgtggt catacccgat gagatcatcc gccgcttgaa
      241 tggcgtccca tcatcacgtc agtgggagga gggcaagcgc atctgcatcg agattatcca
      301 gcaggtgcgc gaagtcgaag gtgtgagcgg cgtgcacgtg atggcctacc ggcaggaaga
      361 gctggtggct gagattattc aggaagccgg gctgctgccg cgtcgtcgga ttagcgagtt
      421 ggggcagtac gacccgtgcg aggagtacac gatttaaact atggaaacga tcatcacctc
      481 acctcagaaa gtggttaaga tcggtccgga gcacccgttt gtggttatcg gggagcgcat
      541 caatcccacc gggcgcaagc tgctggcggc agagatggct gcctggaatt tcgacagggt
      601 gatctcagac gctatcgcac aggtgcaagc gggggcgcgc atgctggatg tgaacgccgg
      661 catcccgctg gcggatgagc cagctatcct ggcagaggca gtgcgcgtgg tgcagagcgt
      721 tgtggacgtg ccgctgagta tcgattcgtc ggtgatcaaa gcgctggagg cgggcctggc
      781 tgcttacaat ggcaagccat tggtgaactc ggtgactgcc gaggaggatc ggctggaagt
      841 ggtgctgcct ctggtaaaga agtacggggc ggcagtgatc ggcatcgcca acgatgagac
      901 gggaatctct gaggacccgg atgtacgctt taccatcgcc aaaaagattg tggagcgggc
      961 ggaagactac ggtatcccgc gccaggatgt gctgatcgac ccgctggcta tgccggtggg
     1021 ggcggtgcgc atggcgggcg tcacgctctt taccctggtg cgcaggctgc gcgatgaatt
     1081 gggcgtgaac acggtgtgcg gagcgtcgaa tatctccttt ggcttgccgg agcggggcac
     1141 attgaacgca actttcctgg caatggcaat ctcggccggg atgacctgcg ccatcaccaa
     1201 cccgctggag gagagcatcc gcaagactgt gctggcagcc gatgtgatga tggggcgcga
     1261 tgagaactgc ctggcatggt tgagcgccca ccgtgaggaa gctccagggg aatcgggcag
     1321 cgcaagacag cgccggcgca gccgtagaga ggaattatcg cgcaagtaaa cattgatgaa
     1381 tttggggggg atttttcgag ggtaagtaga tggcgatcga taaagagaaa atccgcctgg
     1441 cagcagaaca agaaaccctg ctgatcacgc tatattccaa ggtctatggt tgtcctcaaa
     1501 ctgtctttaa agatgagaaa acgcgtcaga ttctggatcg cattgattat gatttttctc
     1561 aattgaaagt ccccacgggg acgcgcctga cggtttgcct gcgcgccaag aagtttgatg
     1621 agattgtgcg ggaattcctt gccagtcatc cgcagggtgt agtgctccat ttgggttgcg
     1681 ggctggacac tcgctttgtc agggttggtt ccccaaggat cgactggtac gacctggatt
     1741 tgcccgaggt gattgacctt agaaagaagt tttttgacga gacagacaat taccacatga
     1801 tcccgtcttc tgtcactgat ctgagttggg tcgaccggat tcctactcgg gatcaggatg
     1861 tcctcgttgt cgccgagggt ttgctgatgt atctaaaaga tgagcaggta aagacgttag
     1921 ttctcaggct gaaagaggct tttccaggat gtgagctggc attcgatgcc tatagcaccc
     1981 tgaccgttag aagagtgaaa gaccacccct ctatcaagaa aaccggggcc ttgatccagt
     2041 ggggcatcga tgatgccagg cagatcgaac attgggcatc tggcatccgt ctaaaagaag
     2101 aatggttcta cacacaatca aaggatatta agaaactcgc ctttagccat cgcttgatct
     2161 tcgcaatcgc cgggctcttt tctgtggcgc gtaaagccca tcgaattctt tattacgcac
     2221 tgtaagaaga gtatgtagaa ggcaattatt tttaacatac aataataggt ctgtgaactt
     2281 atatctctat gtagccgtat atggataggt gtaattacca tgatgatcat ctctaaatct
     2341 tgggagcagg cctaccccgg ggcagtcgtg ggggaattga ggttgggtga tgtagccaac
     2401 ccggaggggc acgctgcgct tgagcagcgc aaagtgaagc tggaagctga tctgcgcgcc
     2461 cgtttctctg gctacgaccg ggctgggctg cgggatatac ccgtcctgca ggcttatcac
     2521 gcctattaca agcgtttcga taagacgtat catgtgcaac tgcaacttga gtcggttgtt
     2581 ctcaagggga aatccatccc gcgcgttgca gcgctggtgg agtgcatgtt tatggctgag
     2641 ctcaagaacc tgctgctgac cgctggtcac gatgcggaca gactgattga tccagtgcag
     2701 cttggtgtgg cgcagggaga cgaggtctac atgcttctta acgggcagga gcaggcgctc
     2761 aaggcgggcg atatgtatat cgcagaccgg gaggctgcca tctccagcgt gatctatggt
     2821 ccagaccggc gcacgcgcat ccgccaagag accaagcggg cgctctttac ggtctacgcc
     2881 ccaccgggga ttggggaaga ggctgtgtgg g
//
LOCUS       DBNHOH010000759         4741 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_46887, whole genome shotgun sequence.
ACCESSION   DBNHOH010000759 DBNHOH010000000
VERSION     DBNHOH010000759.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_46887"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="AC16V7_13665"
     CDS             complement(<1..283)
                     /locus_tag="AC16V7_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258216.1"
                     /translation="MTKWFKYGLVGILVIGALAVLISGTVSAQDDINPPPPHPWFGAG
                     RARGIRLEVAAEALGMTFDELRTQMRDGKTLADLAEGYGPGRMGGSNFGG"
     gene            493..>4741
                     /locus_tag="AC16V7_13670"
     CDS             493..>4741
                     /locus_tag="AC16V7_13670"
                     /inference="COORDINATES: protein motif:HMM:NF019323.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR6258217.1"
                     /translation="MSDYKSQPTSLPSLSTIRIALFAILIVTLACSLPGLFARPTSTL
                     PAEPTPTFTPAPPTPTPQPLPPAIVESSPPIGGEVPLSGPITLYFNQEMDRQSVESAL
                     SLEPGLTGDFTWHDNATLVFAPAEPLTPDSEITFNLETGARSARGLALTQPISLNFRA
                     VGYLRLVQTLPEPDTSEVDPSSAVVASFNRAVVPLGADPANLSPAFSLQPGANGRGEW
                     VNTSTYIFYPDPPLEGGKAYTVAINPELKGVDTSPLDSAEGWTFYTAPPQLSTFLPDP
                     MGGDIRLDSVITMTFNQPMDDQSVQSGFAMLREGSSPVAGKMTWNEDFTALTFIPDNL
                     LQRSTTYTVKLDGGVQARGGTPLVAGFETSLRSVSPLAVVRSEPSQGGTVPQYNGVAL
                     YLNAPVVGKDIVDHIAVTPSVSNLNAWWDEFSRFISIYGDFAPDTDYTLTLLPSMRDP
                     WGGALAGDYTLNFRTSPLPPSIGLSYGYGSNVLFLTPQDSSLIAQVTNFPNVSTSVGS
                     ASLGEFIAMLGPNSFNYAQSFQAADERTWSQSFDLEPNRNTPVDLYVSPQRNPLPLGL
                     YYLGFNTRSETYFPTKYLIVVGNAHLTFKLSDTQSLVWAVNMDDNTPIQGAMVAIYNE
                     NGEAIAQGVTDSQGIFQSEIPALKDPYTNYYAVLGRPIDGVFGIALSNWNQGVASWDF
                     GLISNYTGPRLKAYLYTDRPLYRPGQTVYFRAILRQAYNGRYTIPDLASLPLTLYKNY
                     SEELISFDLPLSAFGTAHGEYTLPIDAPPGDYRIANSIDDYDISVNFRVAEYRKPEIN
                     LQVEFADEQMKSGDDLVARVNARYFFDAPAGNLPVRWTLYTTPLDFYLPGYQVGVQET
                     RWLEAFYYRPFFDQLGYQIQTGEATTDPQGQLTLELPTEVGETRAKYTLEVTMTDESG
                     LPVSARGSIEANPSSYYIGLRPEAWVGRAGEPAGFDVQVVDWEKKPVGPNDLRAEFQT
                     VEWVEEERPPSESFLGPKFVPRYTPVGSTDFQTNAEGQARLSFVPPEPGTYSLSVYSD
                     EARSQVYIWVSGPGSAVWPNLPNQRLRLVSDRDNYKPGDTAQVFVLNPFPSSAQALVT
                     VERGVVMRSEVRTLQPGSNTLSFPLSSEDAPNIYVAVTLLGLDERGGPDFRQGFINLT
                     VEPSEQTLNVALTSQPQRLGPGEDVTFEVRVTDSAGQPVQGEFSLSVVDMAVLALADP
                     NVPDIASYFYGEQPLGVRTGVALAAYARRLTVNPLGGGGGGGDGMIQVVRERFPDTAY
                     WNAEIVTDGDGRATVSLPLPDSLTTWQVLVRGVADDTRVGQTETQVVVTKDLLVRPVT
                     PRFLLVNDHVQLAAVVQNNTPSDLQAEVSIEATGFTLDNPAVSTQSISVPANGRTRVE
                     WWGITQDVPNVELIFSASARSGDTVYQDATRPALGSLP"
BASE COUNT         1017 a         1498 c         1218 g         1008 t
ORIGIN      
        1 cgccgccgaa attgctaccg cccatcctgc cagggccata accctcggcc agatcagcca
       61 gggttttgcc atccctcatc tgggtgcgga gctcatcgaa agtcatcccc aatgcttctg
      121 ctgcgacttc cagcctaatt cctctggctc tgcctgcgcc aaaccagggg tgaggtggtg
      181 gagggttaat gtcgtcttgc gcggataccg ttccactgat caacaccgct aaagcaccaa
      241 taaccagtat tccaacaagc ccgtatttaa accattttgt catctgtagc ctcctatcac
      301 aatcaggtaa tatagttacc caagtaaaac cgtcagtccg tatgcttgca ttcgataaca
      361 ggatatcatt cagttgttca gaaaatatgt agaagtgatt tggatatgat ttagatcctg
      421 tatttgaaag gtataatgaa ccaattggaa caaaagagct cctttatccg tctcttccgt
      481 caggagagag ctatgtctga ctacaaatcg caacctacca gtcttccatc cctatcgacg
      541 attcgaatcg cattatttgc tatcctcatc gtcaccctgg cttgctcatt gccggggctg
      601 ttcgcgcgcc cgacttccac actgcctgct gagcccacgc cgaccttcac gcctgcccct
      661 cccacgccca ctccacagcc gctgcctccc gccatcgtcg agagcagccc accaattggc
      721 ggggaagtgc cgctaagcgg gccgatcacc ctatacttca atcaggagat ggaccgccaa
      781 tcggtggaga gcgcactcag cctggagccg gggctgactg gtgatttcac ctggcacgat
      841 aacgccacac tggtcttcgc gccagctgag cctctcaccc cagatagcga gatcacattc
      901 aacctggaaa ccggtgcccg ctcggcgcgc ggcctagcct tgacccaacc catcagcttg
      961 aatttccgcg ctgtgggtta tctgcgtctg gtgcagacac tgcccgagcc agacaccagc
     1021 gaggtcgatc ccagctctgc ggtggtagcg agctttaacc gcgccgtggt gccgcttggc
     1081 gccgacccgg cgaatttatc gcccgccttt agcctgcaac ccggcgcaaa cggtaggggc
     1141 gaatgggtca acacaagcac ctatatcttc tacccagatc ctcccctgga gggcggaaag
     1201 gcctacaccg tcgccatcaa ccccgagctg aaaggggtcg ataccagccc cctcgattcg
     1261 gcagagggtt ggacgttcta cacagcgccc ccacaactgt ccacatttct gccagacccg
     1321 atgggtggtg acatccgcct ggattctgtg atcaccatga cgttcaacca gccgatggac
     1381 gatcagagcg tccagtctgg ctttgccatg ttgcgagagg gctcttcacc ggtcgccgga
     1441 aagatgacct ggaacgagga ttttaccgcc ctgactttta ttcccgataa cctgctacaa
     1501 cgcagcacga cctacactgt gaagctggat gggggcgtac aagcccgcgg cggcacgcca
     1561 ttagtagctg gcttcgagac ctctctgcgt agcgtctccc cgctagctgt agtgcgaagc
     1621 gagcccagcc aagggggtac agtaccccaa tataatggtg tcgccctgta tctcaacgcg
     1681 ccggtcgtgg gcaaagacat cgttgatcac attgcagtca ccccgtcggt gtccaacctg
     1741 aacgcctggt gggatgaatt tagccgtttc atcagcatct atggcgactt cgcccccgat
     1801 accgattaca ctctcaccct tctgcccagc atgcgcgacc cctggggcgg ggcgttagca
     1861 ggcgactaca ccttgaattt ccgcacatct cccttgccac cttcaattgg attatcttac
     1921 ggttacggca gcaatgtgct cttccttacc cctcaggatt ccagcctgat tgcacaggta
     1981 accaatttcc ccaatgtatc cacgtcggta ggcagtgctt ctttgggcga attcatcgcc
     2041 atgctgggcc caaattcatt caattacgcc cagtctttcc aagccgctga cgagcgcacc
     2101 tggtcacaaa gctttgatct ggaacccaac cgcaacaccc ccgtagacct atatgtctcg
     2161 ccacaaagaa atccactgcc gcttggattg tattacctgg gtttcaacac caggagcgaa
     2221 acctatttcc ccacgaaata cttaatagtg gttggcaacg cccacttgac ctttaagctc
     2281 agcgacacgc aatccctggt atgggcagtg aacatggacg ataacacccc tatccagggt
     2341 gcaatggtcg ccatctataa cgaaaatggt gaggccatcg ctcaaggcgt gaccgactcc
     2401 cagggtatct tccagtcgga gataccggcc ctgaaagatc cctataccaa ctattacgcg
     2461 gtgctcggtc gcccaatcga tggcgtcttc ggcatcgctc tctccaactg gaaccagggt
     2521 gtggcgagct gggatttcgg cctgatcagc aactatactg gcccaaggct gaaagcctat
     2581 ctatataccg accggcccct ctaccgccca ggacagacag tttacttccg cgccatcctg
     2641 cgccaggcgt acaacgggcg ctataccatc cccgacttgg ccagcctgcc gctcaccctg
     2701 tataaaaact actccgaaga gctaatcagc tttgatctgc cactctccgc cttcggcacc
     2761 gcccacggcg aatataccct gcccatcgat gccccccccg gtgattaccg catcgccaac
     2821 tccatcgacg attatgatat ctcggtcaac ttccgggtag ccgaataccg taaacccgag
     2881 atcaacctgc aagtagaatt tgcggatgag caaatgaaat ctggcgatga tctggtggct
     2941 cgtgtcaatg cccgctactt cttcgacgcg cctgccggta acctgccggt gaggtggacc
     3001 ctgtatacta cgcctttaga cttctacctc cccggttatc aggtcggcgt gcaagaaacc
     3061 agatggttag aggccttcta ctatcgacca tttttcgacc agttgggcta ccaaattcaa
     3121 accggtgaag cgaccaccga cccgcaaggc caattaaccc tggagttacc cactgaggtg
     3181 ggtgaaacgc gtgccaagta cacattggaa gttactatga cagatgagag cgggttgcca
     3241 gtcagcgcac gcggcagcat cgaggccaac ccgagttcgt actatatcgg cctgcgccca
     3301 gaagcctggg tagggcgtgc tggggaaccc gctggattcg atgtacaggt ggtagattgg
     3361 gagaagaaac ctgttggccc aaatgacctg cgcgccgaat ttcaaacggt cgagtgggtc
     3421 gaggaggagc gcccccccag cgagagcttc cttgggccca agttcgtgcc aaggtatacc
     3481 cctgttggca gcaccgactt tcagaccaac gccgaggggc aggcgcgcct gtccttcgtg
     3541 ccacccgagc cgggcaccta tagcctcagc gtttacagcg atgaggcgcg cagccaggta
     3601 tatatatggg taagcggacc tggcagcgcc gtctggccga acctgcccaa ccagcgcctg
     3661 cgtcttgttt ccgaccgaga caattacaaa cccggcgata ctgcccaggt atttgtgctc
     3721 aaccccttcc cctctagcgc tcaagcactt gtgaccgttg aacgcggtgt ggtgatgcgc
     3781 tccgaagtgc gcaccctgca gcccggcagc aacaccttga gcttccccct cagctctgaa
     3841 gatgccccca atatctatgt cgccgtaacg ctactcggtt tagatgagcg cggggggccg
     3901 gattttcgtc agggttttat aaacctgacc gttgaacctt ctgagcagac cttgaatgta
     3961 gccttgacca gccagcctca gcgccttgga ccgggcgaag acgtgacctt tgaagtgcgt
     4021 gtcaccgatt ctgcaggcca gcccgttcag ggtgagtttt ccctctcagt agtggacatg
     4081 gccgtactgg ctctggccga cccaaacgtg cccgacattg cctcctactt ctacggcgag
     4141 cagcccctgg gcgtgcgcac cggggtggca ctggctgcct acgctcgccg cttgaccgta
     4201 aatcctctcg gcgggggtgg cggaggtgga gatgggatga tacaggttgt gcgcgagcgc
     4261 ttccctgata ccgcctactg gaacgctgag atcgtcaccg acggagacgg tcgcgccaca
     4321 gtcagcttgc cgcttcccga cagcctgacc acatggcagg tattggtgcg gggcgtggct
     4381 gacgacaccc gcgtcggcca gactgagacg caggtcgtcg tcaccaaaga cttgctggtt
     4441 cgtccggtca cgccgcgctt cctgctcgtg aacgaccacg tccaactggc agccgttgtg
     4501 caaaacaaca cccccagtga cctgcaagcc gaagtctcca tcgaagctac tggcttcacc
     4561 ctagacaatc cggcagtttc cacacagtcc atctccgttc ctgccaatgg gcgcaccagg
     4621 gtggagtggt ggggtatcac ccaggatgtg ccgaatgtgg aattgatctt ctcagcctct
     4681 gcccgctccg gcgacactgt ataccaagac gcaacccggc ctgcccttgg cagcctgcca
     4741 g
//
LOCUS       DBNHOH010000760         3685 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1457980, whole genome shotgun sequence.
ACCESSION   DBNHOH010000760 DBNHOH010000000
VERSION     DBNHOH010000760.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1457980"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1089)
                     /gene="mnmA"
                     /locus_tag="AC16V7_13675"
     CDS             complement(7..1089)
                     /gene="mnmA"
                     /locus_tag="AC16V7_13675"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR6258218.1"
                     /translation="MSNKGRVVVAMSGGVDSSVAAAVLKQQGYEVIGLMLRLWSEPGA
                     EQSNRCCTPDSMAQARRVAAILDIPFYAVDSQETLYSQVVQSFIDGYTQGFTPNPCLV
                     CNRQIRWTFLLDRALALDAQYLATGHYARINRDDQDLYQLRKAIDHSKDQSYVLHALD
                     QERLSHALFPLGDYTKSRVRELARHYDLPVAERADSQDLCFLGDGDYRKFLIRNAPRV
                     SHSGPITTKDGQLLGEHQGLAFYTIGQRKGLGLQSSEPMYVIEKDLQRNTLVVGSRQD
                     LGSSELIASNVNWISGKKPPAPFRAQVKIRYKASEAWANVFPLDDRQVQVLFDEQVRD
                     ITPGQAAVIYNGEVCLGGGIIQSSTG"
     gene            complement(1079..2239)
                     /locus_tag="AC16V7_13680"
     CDS             complement(1079..2239)
                     /locus_tag="AC16V7_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIR6258219.1"
                     /translation="MIYLDYASTTPLDHRVMEAMLPYFSEKFGNTSSVHAYGQQADAA
                     LEKARASIAQMLNCLPAEIVFTSGGSESDNMALRGVAFAQRGKRGATHLLISAIEHHA
                     VSRTAEQLAKLHDFELEYLPVDEFGIVHPHEVAKRLRSDTAIVSVIYANNEIGTINPI
                     DEIGDICMERGIPLHTDAVQAAAYLPIDVQKLKVDLVSLGAHKFYGPKGIGALYIRKG
                     TPFLPSLTGGEQENGLRAGTHNIPYIVGFAESLRLAQVERQERYDRLLPMRDRMVGYI
                     LEEVPNSQLTGHPTERLPNHASFVFEGVDGNALLMMLDVAGYACSSGSACKTGDPEPS
                     DVLIALGLPRSWSLGSLRVTLGKDTTPLEVESFLSDLPRIVKQARSLSEYVK"
     gene            complement(2245..2985)
                     /locus_tag="AC16V7_13685"
     CDS             complement(2245..2985)
                     /locus_tag="AC16V7_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002909732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HIR6258220.1"
                     /translation="MNIEYHKWWSPNLGHDMELKVYGHIGKPMLVFPAQGGRFYEYED
                     FKMIEAISEYIEGGKIKVFTVDSLDQQTWANYAAHPVDRARRHEDYDRYITLEVVPFI
                     RKHCGDYGEKMIATGVSMGAYHAGNFFFRHPDIFDTVIALSGLFQLRSFVGDYVDDNV
                     YFNCPLFYLPNLSDPWYLDQYRRSKIIVCVGQGAWEDAMLADAYALKQILEEKQISAL
                     IDIWGHDVNHDWPWWREMMPYFLETLDL"
     gene            complement(3027..>3685)
                     /locus_tag="AC16V7_13690"
     CDS             complement(3027..>3685)
                     /locus_tag="AC16V7_13690"
                     /inference="COORDINATES: protein motif:HMM:NF024925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR6258221.1"
                     /translation="QELESFFAEKPLVDYIMEEFIGGVICTFDGLTDRDGNMVFYTSH
                     QYSQGVMETVIEDIDIYYYSLREIPKDLEDVGRRVLKAFKVRERFFHFEFFRTEDNRI
                     IALEVNMRPPGGMTTDMFNYACDIDVYREWANIVVHNHFTTDFTRKYHCCYVGRKMKH
                     RYVHSHEHIVSVLGNKLIHHENISGVFSAALGDYGYLIRSQDLNDIYTMADLIQKKAS
                     "
BASE COUNT          968 a          902 c          839 g          976 t
ORIGIN      
        1 tgtgttttat cctgtggatg attgaataat tccgccgcct aaacaaacct cgccattata
       61 aattaccgca gcctgtccgg gggtaatatc acgcacttgt tcgtcaaata atacttgtac
      121 ctgtctatcg tcaaggggga aaacatttgc ccaggcttcg ctagccttat agcggatctt
      181 tacctgggca cggaatggtg caggtggctt cttcccagag atccaattta cgtttgaggc
      241 aattagttcg ctactgccca gatcttgcct gctcccgact acgagtgtgt tgcgttgtag
      301 atctttttca atgacataca tgggctcaga agattgcaac cccaaacctt tgcgctgccc
      361 aatggtgtag aaagccaaac cttgatgttc acccaagagc tgcccatctt tagtggtgat
      421 tggcccagaa tggctgactc gaggggcatt ccgaataagg aattttcggt aatctccatc
      481 accaaggaag cataaatcct gactgtcagc cctctcagcg acagggagat cataatggcg
      541 agccaattct cgcacccgtg atttcgtata gtcaccgaga ggaaaaagtg cgtgacttag
      601 acgttcttga tcaagtgcgt gcaggacgta ggattggtct tttgaatggt cgatggcttt
      661 tcgcaactgg tagaggtctt ggtcatcgcg atttatgcgg gcatagtgac cggtagcaag
      721 gtattgtgcg tccaaagcaa gcgcacgatc gagcagaaaa gtccagcgaa tttgacgatt
      781 gcaaaccaga caaggattcg gcgtgaagcc ttgcgtatag ccatcaataa agctttgaac
      841 tacctgtgaa tataaggttt cttgtgaatc taccgcgtag aaagggatat cgagaatggc
      901 tgctacccgc cgcgcttgtg ccatcgagtc aggtgtacaa caacgattag attgttcggc
      961 tccgggctca ctccacaggc gcagcatcag gccgatgact tcgtaacctt gttgtttcaa
     1021 tacagcggcg gctacagagc tatcgactcc accgctcata gcaactacca cgcgcccttt
     1081 atttgacata ttcgctcaaa ctacgtgcct gttttactat tctggggagg tctgaaagaa
     1141 atgattccac ctccagaggg gtagtgtctt tccccagggt cacacgcagg gaacccagcg
     1201 accatgaacg aggcagacct agggcaatga gcacatccga aggctctgga tctccggttt
     1261 tacaggctga accggatgag catgcatacc ctgctacatc gagcatcatt aataacgcat
     1321 taccatctac cccttcgaaa acaaaacttg catgattggg taatcgttcg gtgggatgcc
     1381 cggtaagctg cgagttagga acctcttcca aaatgtaacc gaccattcga tcacgcattg
     1441 gaagcaacct atcatagcgc tcttgcctct ctacctgagc aagacgaagc gattctgcaa
     1501 atccaacgat gtacggtatg ttatgcgtgc cggctcgcag tccattttcc tgctctccac
     1561 ctgtcaacga cgggagaaat ggcgttcctt tgcgaatgta cagcgcgcca atgcctttcg
     1621 gaccgtaaaa cttgtgtgcg cccagggaaa ctaaatcaac ttttaatttt tgaacatcta
     1681 taggcaggta agcagcagct tgaacggcgt cggtgtggag cggtatcccc cgctccatgc
     1741 atatatctcc gatctcatca atggggttga tcgtcccaat ctcattgttg gcatatatca
     1801 cggaaacgat ggcggtatct gagcgcaagc gcttggctac ctcatgggga tggacgatgc
     1861 caaattcgtc gacaggcaaa tattcaagct caaaatcatg taacttggct agctgctcgg
     1921 cagtccggga tacagcatga tgctcgattg cgctgataag caggtgagtt gcacctcgct
     1981 ttccacgctg agcgaaggct acaccccgca gtgccatatt atcgctctcg gaaccgccag
     2041 aggtgaagac aatctcagcg gggaggcagt tgagcatctg ggcgatgctc gcacgggctt
     2101 tctcaagagc tgcgtcggct tgctgtccat aagcgtggac agatgatgta ttgccaaatt
     2161 tttcactaaa atatggcagc atagcttcca tcacgcggtg atctaatggt gtcgttgatg
     2221 cgtaatccag ataaatcatt tcgtttacaa atctaatgtt tccaaaaaat agggcatcat
     2281 ctctctccac caaggccaat catgatttac atcatgcccc cagatgtcga tcaaagccga
     2341 gatttgcttt tcttcgagaa tttgtttcaa ggcgtatgcg tcagcgagca tggcatcttc
     2401 ccatgctcct tgaccaacac atacgataat tttactacgt cggtactgat ccaaatacca
     2461 gggatctgaa aggttaggaa gatagaatag gggacagttg aaatatacat tatcgtctac
     2521 ataatcgcca acaaacgagc gtaattgaaa taaaccgctc agagcgatga ctgtatcaaa
     2581 tatatccgga tggcgaaaaa agaagttgcc tgcatggtag gctcccatgc ttacacccgt
     2641 agcaatcatt ttttcaccgt aatctccaca gtgctttcga atgaagggta ctacttcaag
     2701 ggtaatgtat cgatcgtaat cttcatgtct acgcgcccta tcaacaggat gggcagcgta
     2761 atttgcccag gtctgctgat ccagactatc cactgtgaaa acctttatct ttcctccttc
     2821 aatatattcg ctaattgcct cgatcatctt gaaatcttca tattcgtaga atcgccctcc
     2881 ctgagcagga aaaacgagca taggctttcc gatatgaccg tagaccttta gctccatatc
     2941 atgacccaaa tttgggctcc accatttgtg gtattcgatg ttcatgcact cctcatgtgt
     3001 atgtgataga cggtatcgag cttcgattat gatgcttttt tctgaataag atcagccatt
     3061 gtgtatatgt cgttgagatc ctgtgaacgt atcagataac cataatctcc taaagctgca
     3121 ctgaagactc cactgatatt ctcgtggtgg atgagcttat tccccagaac actgacaata
     3181 tgctcatgag aatgaacata tcggtgtttc atctttcgac ccacatagca acagtggtat
     3241 ttgcgcgtga aatcagtagt gaaatggtta tgcacaacga tgttcgccca ttctcggtac
     3301 acgtcgatat cacaagcgta gttgaacatg tctgttgtca tccccccagg tgggcgcatg
     3361 ttcacttcta aggcgataat ccggttatcc tcagtgcgga aaaactcaaa gtgaaaaaag
     3421 cgttctcgca ctttgaaagc ttttaataca cgtcgtccaa catcttccag atctttggga
     3481 atctcgcgca aagaatagta gtatatatcg atatcctcaa tgaccgtttc cataactcct
     3541 tggctgtatt gatgggaggt ataaaatacc atgttgccat cccgatcggt caatccgtca
     3601 aaggtgcaga tcacaccacc gatgaactcc tccataatgt aatctaccaa cggtttttct
     3661 gcaaagaagg actctaattc ttgct
//
LOCUS       DBNHOH010000761         1512 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_174282, whole genome shotgun sequence.
ACCESSION   DBNHOH010000761 DBNHOH010000000
VERSION     DBNHOH010000761.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_174282"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1512)
                     /locus_tag="AC16V7_13695"
     CDS             complement(<1..>1512)
                     /locus_tag="AC16V7_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258222.1"
                     /translation="LYHTESPRYLYAAVIFGAATFYSYSNAQLIVLAAATLLFISDVR
                     YHLRNREVVLRGLLLATVLAIPLIEFRLSHPQAIHEHLRVIGSYWTQAIPLQNKLLMF
                     SQKYLYGLSPQYWFLPNSQDLPRHRMAGFGQMQTLVFPLVLLGLAVCIAKWRSSPHRA
                     VILAALATPVGAALLDIGVPRVLAFIIPANLLAAIGLEWIVARWKERLPYRPIAVILF
                     LGLSWANFAMLRTALTQGPLWFQDYGLYGMQYGARQVFEEAIPEYLAKDPEAQILVSS
                     TWANGAENFLQFFITPQELHRVRMDGIDTYLFRKLPLDDRMIFVMTALEYQKASQSPK
                     FNMVTAEKIIPYPDGSPGFYFTRLRYADNVDAIFAAEQEARRQLVTGPVSLDGKLVEL
                     RYSQIDMGEPALIFDNDPFTLMRGLEANPFILEFIFPQPRHVGGVTADLGLINSTLTA
                     KLYTDLDAEPATFTSVFPSANNGDPNIEMPLPDAPERVSKVRIEILDVASGERA"
BASE COUNT          349 a          375 c          451 g          337 t
ORIGIN      
        1 gccctctctc cgctggctac gtcgaggatt tcgatacgaa ctttggaaac tcgctcggga
       61 gcatctggca atggcatttc gatatttggg tcgccgttgt tagcgctcgg aaatacactg
      121 gtgaacgttg ccggttctgc gtccaggtca gtgtaaagct tggcggtcag ggtactgttt
      181 atcaagccca aatccgcagt caccccgcct acatggcgtg gttgagggaa gataaactcc
      241 aggatgaacg ggttggcttc taaaccgcgc ataagggtga agggatcgtt gtcaaaaatg
      301 agcgcgggct ctcccatatc gatctgcgaa tagcgcagct caacaagctt accatccaag
      361 cttacagggc cggtcaccag ttggcgcctg gcttcctgtt cggcagcgaa aatggcatcg
      421 acgttatcgg cgtatctcag gcgggtgaag taaaatcctg ggcttccatc gggatagggg
      481 atgattttct ctgccgtcac catattgaac ttagggcttt gcgaagcctt ctggtattcc
      541 aaggctgtca tgacgaagat catgcggtca tctaggggca gtttacggaa aaggtacgtg
      601 tcgattccat ccatgcgcac ccggtgtagc tcttgaggag taataaagaa ttgtaggaag
      661 ttctctgccc cattagccca ggtggaagag accaagattt gcgcttcagg gtctttcgct
      721 agatattctg gtatagcttc ttcgaagacc tgtcgggctc cgtactgcat gccgtacaag
      781 ccgtagtcct gaaaccacag tggtccttgt gttaaggcgg tgcggagcat cgcgaagttg
      841 gcccaagata gtcccaggaa caggatcaca gcaatggggc ggtagggcag tctctctttc
      901 cagcgagcga ctatccattc cagcccgatg gctgccagca ggttggcggg gatgatgaac
      961 gccagtacac gaggaacacc gatatctaat aacgctgccc caacaggggt agcaagcgct
     1021 gccaggataa ctgcccggtg gggtgaggag cgccatttcg cgatgcacac tgccagtccg
     1081 agcagtacca acgggaacac gagggtctgc atctgtccga agcctgccat gcggtggcgt
     1141 ggcaggtctt gggagttcgg caggaaccag tactgtgggc tgagaccgta caggtatttc
     1201 tggctaaaca taagcagttt attctgcagt ggtatggctt gtgtccagta ggagccaatc
     1261 acgcgaagat gctcgtggat cgcctgcggg tggctcaatc gaaattcaat caacgggatc
     1321 gctagcacgg ttgctaagag cagccccctc agcaccactt ctcgattgcg caggtggtag
     1381 cggacatcgg agatgaagag taacgttgct gcggccagga caatcagctg ggcattagaa
     1441 tacgagtaga acgtggctgc cccgaagatt acggcggcat agagatagcg cggcgactcg
     1501 gtgtgataaa gc
//
LOCUS       DBNHOH010000762         1458 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_880679, whole genome shotgun sequence.
ACCESSION   DBNHOH010000762 DBNHOH010000000
VERSION     DBNHOH010000762.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_880679"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="AC16V7_13700"
     CDS             complement(<1..407)
                     /locus_tag="AC16V7_13700"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR6258223.1"
                     /translation="MFDHFYAIIMAGGGGTRLWPLSRQAKPKQFLRLIEERSLFQTSV
                     QRLEGLFPPERIYVVTVAEQAAELQLQSPAIPKENFLLEPMPRGTASVVGLAAVALQQ
                     RDPQAVMAILTSDHFIGNEEGFRKMLAAAHNAA"
     gene            complement(418..>1458)
                     /gene="dprA"
                     /locus_tag="AC16V7_13705"
     CDS             complement(418..>1458)
                     /gene="dprA"
                     /locus_tag="AC16V7_13705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR6258224.1"
                     /translation="LIQHFGDVETAWHASPQEMRAAGLGPKLIETMQQVRAGVSLELV
                     WERVHSLGIQVLTWEDEAYPRRLKEIDQPPPVLYLRDNLQPEDEWAVAIVGTRGITAY
                     GRQVTAEIASVLARRGVTVISGLARGVDAEAHKAALNAGGRTLAVLGSGVDQIYPPEH
                     RQLADQIIASGALMSDYAPGTPPDGVNFPPRNRIISGLSMAVIVIEAGERSGALITAE
                     FAADQGREVFAVPGNINAPKSVGCNRLIHQGARPLLTPEDVLESLDLASFQQQRVARA
                     VLPKDEVEARLLSVLTREPMHGDDIRARVDRPIEKVSATLALMELKGMVRQLGGMNFV
                     AIREEAEDYIIK"
BASE COUNT          313 a          444 c          378 g          323 t
ORIGIN      
        1 tgcgccgcat tatgagcggc tgccagcatc ttccggaagc cttcctcgtt gcctatgaaa
       61 tgatccgatg tcaggatagc catcaccgcc tgtggatcgc gctgttgcag cgctacagcc
      121 gccaagccaa ccactgatgc ggtgcccctc ggcataggct ccaataaaaa attttccttc
      181 gggatggcag gagattgtaa ttgcagttct gctgcctgct cagcgacagt caccacgtaa
      241 attcgctcag gcgggaataa gccctccagg cgttgcacgg aagtctggaa aaggctgcgc
      301 tcttcgatta agcgtaagaa ctgtttcggc tttgcctgtc gcgaaagtgg ccaaaggcgg
      361 gtgccacctc ccccagccat gataattgcg taaaagtgat cgaacataga tacctgatta
      421 ctttatgatg taatcctctg cctcttctcg gattgccacg aaattcatcc cgcctagctg
      481 ccgtaccatc cctttgagct ccatcagcgc tagtgtggct gagaccttct caatgggcct
      541 gtccacccgg gctcgtatat cgtctccgtg catgggttct ctggtgagca cgctcagcag
      601 cctggcttcg acctcatcct tgggcaagac cgctcgagct acacgttgct gctggaaaga
      661 agccaggtca agactttcca acacgtcctc aggggtcaac agggggcgag cgccctgatg
      721 gatcaggcga ttgcagccca cgcttttggg tgcattgatg tttccaggca cagcgaacac
      781 ctctcgcccc tgatcggcag caaactcagc cgtgatcaaa gctccgctac gctcgcctgc
      841 ctcaataaca ataactgcca tagaaaggcc cgagatgatg cggttgcggg gtggaaagtt
      901 gacaccatcg ggcggtgtac ctggcgcata atcgctcatc aaggcgccac tcgctatgat
      961 ctgatcagca agctggcgat gctccggagg ataaatttgg tcaactccgc ttcccaacac
     1021 agccaatgtc ctacctccag cattcaatgc agccttatgc gcctcggcat cgacgccacg
     1081 tgccagacca ctgattaccg tcaccccgcg gcgtgccaat acgctggcaa tttccgcagt
     1141 cacctgacga ccataagcag tgatccctcg cgtgccgacg atcgccacag cccactcatc
     1201 ttctggttga aggttgtcac gcaggtagag cacaggtggg ggctggtcga tctccttaag
     1261 gcgtctgggg taggcttcat cttcccaggt cagcacctgg atgccaaggc tgtgcacccg
     1321 ctcccaaacc agctctaacg aaacacccgc gcgtacttgt tgcatagtct cgatcagctt
     1381 gggacctagt cctgcggcgc gcatttcttg aggagaagcg tgccaggctg tctctacgtc
     1441 cccaaaatgc tggatcag
//
LOCUS       DBNHOH010000763         4969 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_431903, whole genome shotgun sequence.
ACCESSION   DBNHOH010000763 DBNHOH010000000
VERSION     DBNHOH010000763.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_431903"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="AC16V7_13710"
     CDS             complement(<1..719)
                     /locus_tag="AC16V7_13710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013058025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HIR6258225.1"
                     /translation="MKAIVYEKYGPPEVLQLKEVAKPVPKDNELLIKVHATTVTAADW
                     RMRKPDPVAARLLNGLFRPKKFNILGFELAGEVEAVGKIVKRFKPGDQVFGNTGFGYG
                     AYAEYRCLPEEGDEKKGLIAIKPASIPYEQAAAVSFGGLAALNILRKGNIQSGQKVLI
                     NGASSSTGTYAVQLARYWRAEVTGVCSAANFELVKSLGADKVIDYTKEDFTESRENYD
                     LIFDAVGKMISGISKSKCRKA"
     gene            complement(760..1467)
                     /locus_tag="AC16V7_13715"
     CDS             complement(760..1467)
                     /locus_tag="AC16V7_13715"
                     /inference="COORDINATES: protein motif:HMM:NF025687.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HIR6258226.1"
                     /translation="MNTHRKNAIIVGVLFIIATAFLFIGESVYAPVLTTPDYLETAYP
                     ARITAILGILLEFACVLSIPLIPVFLFPVLRKHSETLALGYLVFRLFEAVLFVLDEIN
                     KLMNISLSQHYLANQGADPANFQTAGSLFQAWNDWSFSFYVFIFAIGAMMLYAVLYRS
                     KLIPRFISGWGFISAVLIFTGVLLGMLELDLNFPGMAFELIFIVPIAVQEMVMALWLI
                     VKGFDPSVISSLSAKQA"
     gene            complement(1501..2364)
                     /locus_tag="AC16V7_13720"
     CDS             complement(1501..2364)
                     /locus_tag="AC16V7_13720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR6258227.1"
                     /translation="MMSRRILVLGGTGMLGQPVVHCLTDKGHTVRILTRSAEKTHNMF
                     GDTVDVVEGTATNRDDIRAALIGCDAVHINLTPVSELAATRHVIELAEDRLERITYVS
                     ATTLSEENRWFDRVDVKLCTEELLRNSGIQHAVFCPTWVMETLHNFIRGNRAVVLHGN
                     NPPELHFFAAADFGRMVADSYDDERALGKRLFVYGPEAITLQDATERFVAACYPTVKV
                     MRWKLWQARLVAKLIRNENLAEVTELIAYLDVAGEHGDPSEANTLFGAPAIALDEWFK
                     MPKDSRKGMPH"
     gene            complement(2679..3293)
                     /locus_tag="AC16V7_13725"
     CDS             complement(2679..3293)
                     /locus_tag="AC16V7_13725"
                     /inference="COORDINATES: protein motif:HMM:NF017959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HIR6258228.1"
                     /translation="MNQKVLYFCGMLVPFTYIFMYILGGALRPGYSHISDSVSELLSP
                     GAPNKKLLDSFNVAFALFYILFGFGVFQFVMGSDHNALVGRIGAGMIIAVGIASIGSA
                     IFPQDATDTPRTLPGTLHLIFVFGVQIPGAIISTLLIGIWMNQADILSGFATYSFISA
                     GAIVLSGALAGPSMGKPFMGVVERISALAVHQWIFFLAWNLLGS"
     gene            complement(3307..4143)
                     /locus_tag="AC16V7_13730"
     CDS             complement(3307..4143)
                     /locus_tag="AC16V7_13730"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR6258229.1"
                     /translation="MKAFVNRYQISIFFILTFVISWFPWYAGIAPEVMAMGPSIAAFT
                     IALIVGGRRGFVDLLRPFGRWRASLGVWGLAIFGPGILYLIGLVVHLLMGGEAPPFIM
                     IREELNLLLLYLFLVVLMPWNGPVGEEFGWRGYVLPKLQNKYSPLIASLVIGTVWGIW
                     HLPSFFAPQGVISALTTALGIGFLIPYSLGTIANSVFMAWLYNKSKASALIAGIIWHA
                     AIDFWAPILLSDSSLVAAREGTHLPTIAPNLYLTVLAVQVVVASVLVIATKGNLGFSE
                     QT"
     gene            complement(4140..4856)
                     /locus_tag="AC16V7_13735"
     CDS             complement(4140..4856)
                     /locus_tag="AC16V7_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HIR6258230.1"
                     /translation="MNTHRRTAILVGIFFIIGTVAGVSSGVVTGPVLGGSDYLAKVAA
                     NESKIALGALLVLVMGFPLAMIPVLMFPIFRKYSEPLALGAVVFRGVLEAVTYMALAV
                     YWLLLIPVSQEFVKAGTADALTFQAMGAFLTKAGYWTEHLLALVFTIGAMMLYWLFYK
                     TKLVPNWLSLWGFIGAILNFVAAIFNMFDPTHPSLSLGVKWGNLIIPLAIQEMVFALW
                     VIVKGFNPDAAILKDRIVAS"
BASE COUNT         1340 a         1292 c         1143 g         1194 t
ORIGIN      
        1 aatgctttcc tacattttga ttttgagatt cccgagatca tttttccaac agcatcaaaa
       61 attagatcgt aattctctct gctttctgtg aaatcctcct tggtgtaatc gataaccttg
      121 tcagctccca gtgatttcac aagttcgaaa ttcgcggcac tgcaaacccc ggtcacttcc
      181 gccctccagt acctggcaag ttgcactgca taggtacctg tactactaga agcgccgttg
      241 ataagaacct tctgcccact ctggatgttc ccttttctaa gaatattcaa tgctgccaat
      301 cccccaaaag aaacggcagc agcctgttca tagggaatac tggccggttt tatggctatc
      361 aacccctttt tttcatcccc ttcttcaggt aaacatctgt actcggcata tgcaccataa
      421 ccaaacccgg tatttccaaa aacctgatca cctggcttaa atcgttttac aattttcccg
      481 acagcttcga cttccccggc cagctcaaaa cctaaaatat tgaacttttt gggcctaaag
      541 agaccattca atagccgagc ggcaaccggg tcaggcttgc gcatgcgcca atccgcggct
      601 gtgacggtgg tcgcatgaac ttttatcaat aattcattat cctttgggac aggtttggct
      661 acctctttga gctggagaac ctctggaggt ccatattttt catatacaat cgctttcatt
      721 taggcatcct tcctttttag gtgagtctcg ttcatatcat taagcctgtt tagcagagag
      781 agaagagatt acagatggat cgaagccttt gacgatgagc cacaacgcca tcaccatttc
      841 ctgcacggcg atagggacaa taaatattag ttcaaaagcc atacctggga aattgagatc
      901 gagttccagc attcccagga gcacaccggt gaaaatcaat accgcgctga taaaacccca
      961 gccagagata aaacgaggaa ttagttttga ccgatagagg acagcataaa gcatcattgc
     1021 cccgatggcg aagatgaaga cgtaaaacga aaaggaccag tcgttccagg cctgaaataa
     1081 gctgcccgcc gtttgaaaat tggcaggatc cgcaccctga ttggccagat aatgctggct
     1141 taaactaatg ttcatcagtt tgttgatctc gtccagcaca aagagtacag cctcgaaaag
     1201 tctgaagacg aggtatccta gggccaatgt ttcgctgtgt ttccttaata ccgggaacag
     1261 gaataccggg atgagaggga tggaaagcac acaggcaaac tccagcagga taccaaggat
     1321 ggctgtgatg cgagcgggat aagccgtttc caggtaatct ggtgtggtaa gcacaggagc
     1381 ataaacagat tctcctatga acagaaaagc ggtggcgatg atgaacaaca ccccaacaat
     1441 gattgcgttt tttctgtgcg tattcatttt ctgctccttt tattcgtctc tattacccat
     1501 tcaatgcggc atgcccttgc ggctgtcttt gggcatcttg aaccactcat cgagggcgat
     1561 ggctggggcg ccgaagagcg tattggcctc ggaagggtcg ccatgctcgc cagctacgtc
     1621 gaggtaggcg atcaactccg tgacttcagc caggttttcg ttgcgaatca gttttgcaac
     1681 gagccgcgcc tgccagagtt tccagcgcat cactttgacg gttggataac aggcggcgac
     1741 gaagcgctcc gtcgcatctt gcagggtgat ggcttccggg ccataaacga acagtcgctt
     1801 cccgagcgcc cgttcgtcat cataagaatc ggccaccatc cgcccgaagt cggcagcggc
     1861 aaagaagtgc aactccggcg ggttgttacc atgtaatacg actgccctgt tgccccttat
     1921 gaaattatgc agtgtctcca tcacccaggt ggggcagaag accgcgtgct ggatgccgct
     1981 gttccgcaag agttcctcgg tgcataactt cacgtcaacc ctgtcaaacc agcggttttc
     2041 ctcgctcagt gtagtggctg agacataggt gatgcgttca agtcgatcct ccgccagttc
     2101 aatgacgtgc cgtgtggcgg ccaactccga cactggcgtc aggttgatat gcacggcgtc
     2161 acatcctatc agcgccgccc ggatatcgtc tctgttcgtt gccgttccct cgacaacgtc
     2221 gacagtgtcg ccaaacatat tatgggtttt ttcggcgctg cgggtgagaa tccgtaccgt
     2281 gtgtccttta tcggtcaggc agtgcacgac cggctgaccc aacatgcctg tcccacccag
     2341 aaccaagatt cgtcttgaca tcatctacac ctttcaaact ttgcaggagt agcctccagg
     2401 gccactctgg ctgttgacct ccccttacat ctttttcagc caacaaagtt tcacttacta
     2461 cttgaatcta cgtagttatt attgaattga acccaaataa aactgtggtc cctgtctaca
     2521 tccttgatgt gattactcaa tcgcaggcaa ttcactctgt tggtgcctgc gcaaaagtct
     2581 ctggggaaat tgcagttggg tcaaatccct tcacggtcaa ccacagcgcg aataccattt
     2641 cttgaatagc caacagagtt atcaatatat ctccgttgct agcttcccaa cagattccaa
     2701 gccaaaaaaa atatccattg atggacagcc aaggcagaga ttcgttcaac tacacccatg
     2761 aatggcttcc ccatggatgg gcccgcaagt gcacctgaaa gaacaatagc acctgctgaa
     2821 ataaacgaat aggttgcaaa ccctgagaga atatccgctt gattcatcca gattcctatc
     2881 aacaacgttg aaattatggc gccggggatt tgaacgccaa acacaaatat caagtgcagg
     2941 gttccaggca aagtgcgtgg agtgtctgtc gcatcctgag gaaagatggc actcccgata
     3001 ctggctatcc caacagcaat gatcatccca gctccgatac gacctaccaa tgcattatga
     3061 tcacttccca ttacaaactg gaatactcca aatccaaaca atatgtaaaa caaggcaaag
     3121 gctacattga aactatccag tagtttcttg ttgggcgctc ccggcgaaag taattcactc
     3181 actgaatccg aaatgtggct gtaacctggg cgtaatgccc caccgaggat gtacatgaag
     3241 atataagtga atggcacaag cattccacaa aagtacaaaa ctttctggtt cattatgttt
     3301 ccattatcaa gtttgctctg aaaagcccaa attccctttt gtggcaatta ccaagacgga
     3361 agccaccacg acttgaacag ccaaaaccgt tagataaagg ttgggtgcga tggtgggaag
     3421 atgagttcct tcacgagcag cgacaaggga tgagtcgctt agaagtatag gcgcccaaaa
     3481 gtcgatcgct gcatgccaaa tgattccggc aatcagcgcg ctggctttgg atttgttgta
     3541 cagccaggcc atgaaaacag aattggcgat cgttcccagg ctataaggga taagaaaacc
     3601 gattcccaat gctgttgtga gcgcgctgat cacaccctga ggggcaaaga aactgggcag
     3661 atgccaaatt ccccagacgg tgccgattac caggctggca atcaatgggc tgtatttatt
     3721 ctgcaatttg ggaagcacat agccacgcca gccgaattct tcgcccactg gcccattcca
     3781 aggcatcaaa acaacaagga aaagatatag taggagcagg tttagttctt cgcgaatcat
     3841 gatgaacggg ggagcttcgc cacccatgag caagtgcaca acgagcccaa ttaggtataa
     3901 aatccctggt ccaaagatag ctagccccca tacacccagg ctggctcgcc agcgtccgaa
     3961 cggtcgcaga agatcgacaa atccgcgcct gccgccgaca atgagcgcta tggtgaaggc
     4021 ggcgatggat ggacccatcg ccatgacttc cggtgcgatt cccgcatacc agggaaacca
     4081 actgataaca aaggtgagga tgaaaaatat ggagatttga tatcgattga cgaaagcctt
     4141 catgatgcga caatcctgtc cttcaagata gctgcatctg gattgaatcc cttcacgatc
     4201 acccacagcg caaataccat ttcctgaatt gccagcggga taatcaagtt gccccatttg
     4261 acgcctaggg aaagagacgg gtgtgttggg tcaaacatat tgaaaattgc cgcgacgaaa
     4321 ttcaagatcg ctcctatgaa accccataac gacagccaat ttggaacaag tttggtttta
     4381 taaaagagcc aatacaacat catagcgccg atggtgaaaa ccaacgccag caggtgctcg
     4441 gtccagtaac cagctttggt gagaaaagcg cccattgcct gaaaagtcag tgcatccgct
     4501 gtgcctgcct ttacgaattc ctggcttacc gggatgagca aaagccaata gacagcgagc
     4561 gccatatagg tcacggcttc caatacgccc ctaaaaacaa ctgcgccaag cgccaagggt
     4621 tcgctgtatt tgcgaaagat cgggaacatc aggaccggaa tcatcgccag ggggaaaccc
     4681 atcaccaaca ccaggagggc gcccagggcg attttgctct cattcgctgc gactttggca
     4741 aggtaatcag aaccacccaa aaccggaccg gtaacaacgc cgctcgaaac gccggcaact
     4801 gttccaatga tgaagaatat gcctacaagt attgctgtcc ttctgtgtgt gttcatttta
     4861 ttttctcctt tcaggtagtc cctttttgat tttctgaact accttctatg taactactgc
     4921 agggattagg cctttctctc tacttctgag cagttaccct tccagaact
//
LOCUS       DBNHOH010000764         3588 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1393387, whole genome shotgun sequence.
ACCESSION   DBNHOH010000764 DBNHOH010000000
VERSION     DBNHOH010000764.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1393387"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2089)
                     /gene="lon"
                     /locus_tag="AC16V7_13740"
     CDS             complement(<1..2089)
                     /gene="lon"
                     /locus_tag="AC16V7_13740"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR6258231.1"
                     /translation="MRNHDWFTRNDDDDEFGEALHRRTEELYDVPDAIPDEDGLIECP
                     VLPLRDLVVFPRMVSPIFIGREASLLAIEEAQMKDQTLIALVQRDPEQDDPGPSDFLG
                     VGVELAVGRLLNMPDGSNSALVQGRRRVELIEFTQLEPYLRARTRPVYESVDVDRQTD
                     ATMRTVLELFQKCVNLDRSLPEEAYLYALNVEEPGWLADMIVTAIAPPLEERQTILES
                     VDPLERLRQVIKLLAREVDVLELEDEIHTRAQSEVDRTQREYYLREQMKAIQTELGEG
                     DLWSRDIQELRTRLESANLPEEVQVRAIKELDRLGQMPPMSPEVGILRTYLEWILELP
                     WTHVTEDNLDVKHAAKVLERDHFGLPRAKERVLEYIAVRSLKPKRMRQPILCFVGPPG
                     TGKTSLGRSISEALGRKFVRLSLGGVRDEAEIRGHRRTYIGALPGRILQTMRRAGTAN
                     PLFMLDEIDKLGQDFRGDPAAAMLEVLDPEQNHAFSDHYLELPYDLSKVMFITTANTV
                     GTIPPALLDRMEIIEFPGYIEEEKLEIATRFLIPRQFEESGLDSGELRLSTQALQRII
                     REYTYEAGVRNLEREIGRICRKIARLKAEGKRHPNQVSANVIERYLGPPQFFNMEAEL
                     KNEVGVATAVAWTENGGEIMPVEVLLMDGKGNLQITGQIGNVMQESAQAALSYLKSRA
                     KALDLDAEIFDQVD"
     gene            complement(2089..3432)
                     /locus_tag="AC16V7_13745"
     CDS             complement(2089..3432)
                     /locus_tag="AC16V7_13745"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIR6258232.1"
                     /translation="MTTEDQYQTTLDYLYSFVDFSLTKGMRYTPEQFDLGRMRAFVKA
                     LGNPERAYPSIHVAGTKGKGSVSSMCSAVLQAEGYRVGLYTSPHLHDYAERIRINQVS
                     IPHQDLISLVEEIKPVIEAVPGVSTFEITTALAFLYFQHQKVDVAVLEVGLGGRLDAT
                     NVVEPSLAVITSLSFDHTQILGNTLSEIAREKAGIIKAGIPVVVSPQDDEARKVIEQI
                     ALERSAPLIQVGRDYSYAALHHSLEGQSLQVWNGTEKRDAEPLAIPLLGMHQVENAAT
                     AYAALQVFSQRALPVSLGSIKRGFTQLTWPGRFEILPRQPPVVVDSAHNRDSAKRLRQ
                     ALDDYFPDMPVVMVFGASEDKDIAGMFAELSPRVQDVVATKSIHPRAIEPEHVVEIAH
                     QNGIPAKIVADIPEAVMVALELADGDKLTLVTGSIFVVAATREAWMARAVNLDNG"
BASE COUNT          828 a         1029 c          879 g          852 t
ORIGIN      
        1 cgtcaacctg gtcgaatatc tctgcatcca gatcaagagc cttcgcacgc gacttcaaat
       61 acgaaagcgc agcctgggct gattcctgca tcacgttacc gatctgtcct gtgatctgca
      121 ggttaccctt cccatccatc agcagcacct caaccggcat gatctctcca ccattttccg
      181 tccaggcaac cgcggttgcc acacccactt cgtttttcag ctcggcctcc atgttaaaaa
      241 actgtggggg tccaaggtag cgttcaatta cgttggcgct cacttgattg gggtgacgct
      301 taccttccgc cttcaatcga gcgatcttgc gacagattcg cccaatctcc cgctctagat
      361 tgcgcacccc tgcttcatag gtatactctc tgataatccg ctgtaacgcc tgagtagaca
      421 gacgcagctc cccggaatcc aaaccgctct cttcaaactg tcgcggtatg agaaagcgtg
      481 ttgcgatctc cagcttctcc tcttcaatat aacctggaaa ttcgataatc tccatgcggt
      541 ctaagagtgc cggtggaatc gtgcctacgg tattggcagt agtgatgaac attaccttgg
      601 aaaggtcata tggcagctca aggtaatggt ctgaaaaggc gtggttttgc tccggatcca
      661 gaacttccag catggctgcc gccgggtcgc cacgaaaatc ctgacccagc ttatcaatct
      721 catccaacat gaaaagggga ttcgccgtgc ccgcgcgccg catagtctgt aagatacgtc
      781 cggggagcgc accaatatag gtacgacgat ggccgcgtat ctcggcctcg tcacgcacgc
      841 cgcccaggga aaggcgaacg aacttgcggc ccagcgcctc ggaaattgag cgccccaagg
      901 atgtcttgcc tgtaccgggc gggccaacaa agcagaggat gggttggcgc attcgcttgg
      961 gtttcaggct gcgcactgcg atgtattcca gcacccgctc tttggcacgc ggcaggccaa
     1021 aatggtctct ttcaagcact ttggcagcat gttttacatc cagattatcc tcggtcacat
     1081 gcgtccaggg gagttccaaa atccactcca ggtaggtgcg caagattccc acctcaggcg
     1141 acatgggggg catctgtccc aaacgatcga gctctttgat ggctcgcacc tggacttcct
     1201 cgggcaaatt agccgactct aatcgggtgc gcagttcttg aatatcgcgc gaccagagat
     1261 ccccctcccc caattctgtc tgaatagcct tcatctgctc acgcaggtag tattcacgct
     1321 gtgtgcgatc tacctcgctt tgggcgcggg tgtgaatttc atcttccagc tccaagacat
     1381 caacttctct cgccagcagc ttgatcacct gtctaaggcg ctcgagcggg tcaacagact
     1441 ctagtatcgt ttgtcgttct tccaggggag gggcgatcgc agtaacaatc atatcagcca
     1501 gccagccagg ttcttcaaca ttcaatgcat acaggtaggc ttcttctggg aggctgcgat
     1561 ctagattgac gcacttctga aacagctcca atacggtgcg catggtagcg tcagtctggc
     1621 gatccacatc gaccgattcg tagactggtc gcgtgcgggc gcgcaggtaa ggttccaatt
     1681 gagtgaactc gatcagctcc acccgcctgc ggccttgcac cagcgccgaa tttgagccat
     1741 ccggcatatt aagcaaccga ccaacagcca attccacgcc aacgcccaaa aaatccgatg
     1801 ggcctggatc atcctgctca ggatcgcgtt gcaccaaggc gatcaacgtc tgatccttca
     1861 tctgggcttc ttcaatagcc agcaatgagg cctcgcggcc gataaaaatc ggggaaacca
     1921 tgcgcggaaa tacgaccagg tcgcgcaaag gcagcactgg acactctatc agcccatctt
     1981 catccggtat ggcatccggc acgtcataaa gctcctcggt acgccggtgt agcgcctcac
     2041 caaactcatc atcatcgtca ttacgtgtaa accaatcgtg atttctcatt atccattatc
     2101 caaatttacg gcgcgcgcca tccaggcctc gcgcgtagcc gctacgacaa aaatactgcc
     2161 ggtgaccagg gttaatttgt caccatcggc tagttctaat gctaccatca ctgcttctgg
     2221 gatatccgct acgattttcg ctggaatacc attttggtgt gcaatctcta ccacatgctc
     2281 aggctcgatg gcgcgaggat gaatcgactt ggtcgcaacc acatcttgaa cccgcggact
     2341 cagctcagcg aacatacccg caatgtcttt atcttctgaa gcgccgaaca ccatgacaac
     2401 aggcatgtct gggaagtaat catctaatgc ctgccgcaac ctctttgccg aatcgcggtt
     2461 atgtgcggaa tctaccacaa caggcggttg ccgcgggaga atctcaaagc gaccgggcca
     2521 tgtaagctga gtaaagcctc gcttgatcga gcctagactc actggcaacg ccctctgaga
     2581 gaacacctgg agagccgcat aagcagttgc agcattctca acctggtgca tgcctagcag
     2641 aggaatagcc aggggttcgg catctctctt ttctgtccca ttccacacct gcagggattg
     2701 cccttccagt gaatgatgta atgcggcata ggaataatct cgaccaacct gtattaaggg
     2761 agcagatcgc tctaatgcaa tttgttcgat tactttccgg gcttcgtcgt cttgcggaga
     2821 gacgactacc gggatacctg ccttgatgat cccagccttc tcgcgtgcga tctcggagag
     2881 cgtatttcct agaatttgcg tgtgatcaaa ggagagcgac gtgatcacag ccaaggacgg
     2941 ttccactacg ttggtggcat ccagccgacc gcccaagccg acttctagta cggcgacatc
     3001 aaccttctgg tgttgaaaat aaagaaaagc cagcgcagtc gtgatttcaa atgtggacac
     3061 tccaggcact gcttcgatga ccggcttgat ctcttcaacc agcgaaatca aatcctggtg
     3121 gggtattgag acctggttga tgcggatgcg ctcggcataa tcgtgcaggt gcggtgatgt
     3181 atacagacct acccggtatc cttcagcttg tagcactgca ctacacatgg atgacacaga
     3241 ccctttccct tttgttccag ccacgtggat gctagggtaa gctcgctccg gattacccag
     3301 agccttaacg aaagcgcgca tacgtcccag gtcaaactgc tccggggtat agcgcatacc
     3361 ttttgtcagg ctaaaatcga caaagctata aaggtaatca agtgttgtct ggtattgatc
     3421 ttctgtagtc atcgtggtgg gattgtaacc gtatgatccc tagtgtgcaa gaggaagtac
     3481 ctcgatttaa ttcgcgtgat acaattcgcc cgtgttgcga tttgcaaaaa tgttactccg
     3541 catcatcctt ctattggcct cagccggcat tctgactctg ctcatccc
//
LOCUS       DBNHOH010000765         1504 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_47246, whole genome shotgun sequence.
ACCESSION   DBNHOH010000765 DBNHOH010000000
VERSION     DBNHOH010000765.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_47246"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="AC16V7_13750"
     CDS             complement(<1..1268)
                     /locus_tag="AC16V7_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258233.1"
                     /translation="MIKLPYKFNWQNPVLRRAMYPLREQKLREFLLIYDEADLVKKHA
                     SKPPAERVPQVKAELQTFRQRYENMQHDELIEELLERFDADTTRQRYPDWLRYATVHF
                     SGIRYKSAHGSWANPNLLITKLEEMARDTLKKASPEQITEMAQKAGLKNLPLDLEAKK
                     NALFSYLTAQEVAAPSQSLESVNLVALERLLLMKERMEFPHWFWKEVVSHTDLRLAIQ
                     KGDWLNDWENPSLEDSRERQQTKDFRWQSIMFSWKQDITAWRQKHYSDLSLVVTRVVC
                     NELSEHIHHVRGIKPASGLTSKPTWYVNLMGRPAPPPKPEKPGMAKVKASLKKKKESP
                     PPPIQDLPDGEFYFGQALNRTYFKPGASILSLGWVSIKPNPWQIARPISGIDFNEEVD
                     PSWKYIVFNNEFTRTKPAPPPPGQPQKKHK"
BASE COUNT          324 a          348 c          401 g          431 t
ORIGIN      
        1 tctttgtgct tcttctgggg ttgccctggg ggaggcgggg cgggctttgt acgcgtgaac
       61 tcgttattga acacaatgta tttccagcta ggatcaactt cctcgttaaa atctatacca
      121 ctaatagggc gcgcaatttg ccaggggttg ggtttgatag atacccatcc cagggacaga
      181 atgctggcgc caggcttgaa gtatgtgcgg ttgagcgcct gtccaaaata gaattcgcca
      241 tccggcaggt cttgaatggg agggggaggg ctctcctttt tctttttcag ggaggcttta
      301 acttttgcca taccgggttt ttcaggcttg gggggcggtg caggtctgcc catcaggttc
      361 acataccagg ttggtttgga tgtcaggccg gaagcgggct taatcccgcg tacatgatgg
      421 atatgctcgc ttaactcgtt gcacaccacc cgtgtgacca ctaatgagag atcactatag
      481 tgcttctgtc gccatgcagt gatatcctgt ttccagctaa acatgatact ctgccagcgg
      541 aaatccttgg tttgctggcg ttcgcggcta tcttccaggg aaggattttc ccaatcattg
      601 agccagtcgc ccttttgaat tgccaggcgc aggtcggtat ggctgaccac ttctttccag
      661 aaccagtggg ggaactccat gcgctctttc atcagcagca ggcgctccaa tgcgaccagg
      721 ttgacgctct caagggattg gctaggtgct gcaacctctt gagcggtcag atagctaaaa
      781 agggcgttct ttttagcctc aaggtcaagg ggtaggttct ttaagccggc tttttgagcc
      841 atctcggtga tctgctcggg agaggctttt ttgagggtgt cgcgcgccat ctcttcaagt
      901 ttggtgatca acaagttggg atttgcccac gagccatgcg cactcttata gcgtataccc
      961 gaaaagtgta cggttgcata gcgcaaccaa tcagggtagc gctggcgtgt cgtatcggca
     1021 tcaaagcgct ccaatagctc ctctataagc tcatcgtgct gcatattttc atagcgctgg
     1081 cgaaaggttt gcagctctgc cttgacctgg gggaccctct cagcaggggg tttggaggca
     1141 tgttttttga ccaggtcagc ttcgtcgtaa atgagcaaga attcacgtag tttttgttca
     1201 cgcaacgggt acatggcgcg gcgcagaacg ggattttgcc aattaaactt ataaggtaat
     1261 ttgatcatcg tagttttctc caaaagctcg accaacaggg tgcttgataa ttatcgccgt
     1321 actcgtttac ttatctcgca tcgtgtctgg tttgatgggt acgcacatta tattgtacaa
     1381 gatcaaattt ggggttattg tagcggaaat tacttctttg cgatttttcc acatggattg
     1441 agatgaaact ctcaaaaatc gatgagtttc tggatcttag ccatttgctt tcgaccatcc
     1501 aaat
//
LOCUS       DBNHOH010000766         1671 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_816258, whole genome shotgun sequence.
ACCESSION   DBNHOH010000766 DBNHOH010000000
VERSION     DBNHOH010000766.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_816258"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="AC16V7_13755"
     CDS             <1..601
                     /locus_tag="AC16V7_13755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6258234.1"
                     /translation="PGLIPARVNEKMGARYMNNGEIIFENCRVPEDHLLVKDIALRKA
                     GVYFRPGKILQAAKNLGVGVAAYEDTAAYVQQYVQGGKILIKHQAVANKLADMAIKIE
                     TTRTFTRYAARALDEGASDAEKLCLMAKTYAADAIFEVCKDAMELHGGGGVMVEVGIE
                     KHFRDAAVYLHMDGTQDIHRFKIIKTIYPETAGVYAGAD"
     gene            624..1646
                     /locus_tag="AC16V7_13760"
                     /pseudo
     CDS             624..1646
                     /locus_tag="AC16V7_13760"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
BASE COUNT          408 a          413 c          485 g          365 t
ORIGIN      
        1 acccggtctt atccctgccc gcgtcaacga gaagatgggg gcgcgctaca tgaacaatgg
       61 cgagatcatt tttgaaaact gccgagttcc tgaagatcac ctgctggtca aggacatagc
      121 ccttcgcaaa gcgggtgtat acttccgccc ggggaagatc ctgcaagcgg caaagaattt
      181 gggagttggt gttgctgcgt atgaggacac tgccgcatat gtccagcaat atgtacaggg
      241 tggaaagata ctcatcaagc accaggcagt ggcgaacaaa cttgctgaca tggcaataaa
      301 aatcgagacg acgcgcacat tcacgcgcta cgccgctcgc gccttagacg aaggcgcttc
      361 ggatgcagaa aaactgtgct tgatggcgaa aacctatgcg gcggatgcca tatttgaggt
      421 ctgcaaagat gccatggaat tacatggcgg cggtggcgtc atggtcgaag ttggcatcga
      481 aaagcatttt cgagacgcgg ctgtatattt gcacatggat ggcacgcagg atatccaccg
      541 ctttaagatc ataaaaacca tttaccctga aactgcaggg gtttatgctg gtgccgattg
      601 accatgacca ggatgatagt gtcatgagca aaccacttgg cgtgaccgtg atcggcacag
      661 gtatgtgggg aaaacacatg ctggcggctg tcaagcgaac gccgtccctg cgcctggtag
      721 cctgttacag ccgcgatgtg gctgcccgag aagctgtagc agctgagttc ggctgcgctg
      781 cgccagagac attcgagcag gctatcaact ggggtggagt ccaaggtgtg cttctgatca
      841 cgcccaatga tgtccatgcg gagcaggctc tagagtgcgc tcaatgggga aagcatatct
      901 ttgtcgagaa acccatcgcg tactcgttgg aagatggcat agcgatgcat tcggcgtgca
      961 aatcggcagg tgtaacactg tttgtagggc atggctttcg gcgttcgggt gccgcacgca
     1021 aggtgaagca aatgttggat gaaggcaagc tgggacaggt tgtgctggca gaagcgaact
     1081 tttccttgcc aggtaccctc acgcccgaca aatggcgcta ttatcgccaa acttgtccag
     1141 gcggcccgct gatgcaactg ggcatccacc atgctgatac cttgcagtac tggctgggac
     1201 cagtcaagcg tgtccgtggc acctttgccc acttggcaac ctccgctgag attgacgatg
     1261 ttgggattgc acaactctaa tgttccaacg gtgcgcttgg cacgttgaca ggaagttacg
     1321 tatctcccaa aacatactac ctgcgccttt acgggacgga gggagtgctc gattaccaaa
     1381 cagatatgag aatctggcca aatgccgacc agatggattc agccaccgtt ttgactctca
     1441 ggaccaagtc tgcagtagag cagctggaat ttgagtcgcg tgacatgctg gtggatgaat
     1501 tagatgaatt tgcccgttgc atccagggtt cagcccagcc tgagacgggc gcattggaag
     1561 ggctgcgggc gctggatgta attttgtccg ccatccgctc tcaagaaacg ggaaccccta
     1621 ttgcactagg agaatacaat gcctgatgaa cgagttgaag ctgccatcaa g
//
LOCUS       DBNHOH010000767         3325 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_368014, whole genome shotgun sequence.
ACCESSION   DBNHOH010000767 DBNHOH010000000
VERSION     DBNHOH010000767.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_368014"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="AC16V7_13765"
     CDS             complement(<1..564)
                     /locus_tag="AC16V7_13765"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014759.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HIR6258235.1"
                     /translation="MPPVVYLIDGHALAYRTYFALTRGSSQGFTTSKGEPTAGVFGFT
                     SVLLRILEQERPDYLAVAFDTGKTFRDELFPEYKGTRAKMPDDLRPQIERIRQLVDAF
                     NIPRLEVENYEADDVLGSVAHWAVENGLGVKIITGDRDLLQLVGERVIVNLPGKSLSE
                     ARDYLPKDVAEVMGVQPEQVVDFKALVG"
     gene            777..2393
                     /locus_tag="AC16V7_13770"
     CDS             777..2393
                     /locus_tag="AC16V7_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258236.1"
                     /translation="MPSVSRAIKIFVIFLVVIGAAIWITYRVAADEPGRQMTIVIPYI
                     EYEYWLLSWQENEVECRVVIDHEGLPTSQDVLKSCGEGVQTEWLNTPSCNKIIKGKGK
                     ISSCPGLYLIQISSQPKEKEITIDLPQASVGVNLEGCSPVPPENLCEKLPALLLTGEE
                     PLPNEQITSIEGTINGVPFNCKGATCPVKLTITSDIGVTVEFWANSTFGDSSPRYTAQ
                     VRVIDAGVSLVPGGGGFHIDVLSSQWKGGPLASCSRTWETFPPVGGTVDWLRSPNQPA
                     LLVSDEAYYYLAGRLIAQGVVDASKCPSLGLLPNGYANACGLEVAAPIVMAWQNQFDA
                     RIIQVSKETGVPAQLLKNLFAQESQFWPGMFKVPFEFGLGQITDKGADTILLWNTDFY
                     ETFCPLVLSKKACDNGYLKLQVDDQAMLRGALAQQAKSDCPLCPAGVDLTSANSSISL
                     FANTLLSSCDQVAQIVHNATGEKPGAVSSYEDLWRFTIANYHAGPGCLSYAIYATWAA
                     NGQELLWDEVTTQFTEACKGVIPYVDKIAR"
     gene            complement(2397..2585)
                     /locus_tag="AC16V7_13775"
     CDS             complement(2397..2585)
                     /locus_tag="AC16V7_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258237.1"
                     /translation="MAKEKDLPEPSTDVIAETDNYVAWRAKEPDGETTYHLELASVTV
                     HFFQEEWDEFLELVEALV"
     gene            complement(2737..>3325)
                     /locus_tag="AC16V7_13780"
     CDS             complement(2737..>3325)
                     /locus_tag="AC16V7_13780"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR6258238.1"
                     /translation="FEAKIDDILNWSGNNLDLTEPTKSPGEWVMVPGGHREFRQWIIP
                     VIPRGAAGVSKSVYGAGACEGGYEGAFGSGGFIWPAGNHSLSGNDYWSGHLGIDIAAG
                     EGAPIYAADSGVVVFSGWATGGYGNMVMVDHGNGYQSVYAHLSNASASCGRSVGQGQI
                     IGYSGSTGNSTGAHLHFEVRYLGGFVSPWYVLPAP"
BASE COUNT          813 a          892 c          825 g          795 t
ORIGIN      
        1 cccaaccagc gctttgaaat cgacaacctg ctctggctgt acacccatga cctcagccac
       61 gtctttcggc agatagtcgc gcgcctccga cagcgattta cccggtaaat tgacgatgac
      121 ccgctcgccc accaactgta ataggtcacg atcaccagtg atgatcttga ctcccaatcc
      181 attttctacc gcccaatgtg ccacgctgcc gagaacatca tcggcttcgt aattctccac
      241 ctccaggcga gggatattga aggcatctac cagctggcgg atacgctcga tttgcggtct
      301 cagatcatcg ggcattttgg cgcgcgtgcc cttatattcc ggaaacagct cgtctcggaa
      361 ggttttgccg gtatcaaagg cgactgccag gtaatcgggg cgctcttgtt ccaggatgcg
      421 cagcagcacg ctggtaaagc caaaaacgcc agcagtcggc tcacccttgc tggtggtaaa
      481 cccttgcgag gagccgcggg taagtgcaaa ataagttcgg tatgccagtg cgtgaccgtc
      541 gattaaatag acaacagggg gcataagcta cctacggtat aagagtgtat cacgggcttt
      601 agacagccgc aaggacgttc taaccaatat gtaatgcccg tatttgtcaa cgcgaataaa
      661 atattacctc aatcatagag gaagtgtaac gaacagcctg cacgttcgtt ctaataataa
      721 aggcttaaaa ttccctgatt gacaatagtg cctgatcatg atatttttgt tgtaagatgc
      781 ccagtgtctc gcgcgccatc aagatctttg tgatcttcct tgtcgttatc ggagcagcga
      841 tctggatcac ttatcgcgtt gctgcggatg agccgggacg ccaaatgacg attgtcattc
      901 cctatatcga gtatgagtac tggctcttat cctggcaaga gaatgaagta gaatgccggg
      961 tggtgataga tcacgagggg ttaccaacca gtcaagatgt gctcaagtct tgcggggagg
     1021 gtgtacaaac agaatggctg aacacgccgt catgcaacaa gataattaag gggaaaggca
     1081 agatttcctc ttgtccaggc ctatatctaa tccagatctc ctctcagccc aaagaaaaag
     1141 aaatcacgat cgaccttccc caggcttcag tcggagttaa cctggaaggg tgctctcctg
     1201 tcccaccaga gaacctgtgc gagaaattgc ctgcattgct gttaactggg gaagagcccc
     1261 ttccaaacga acagattaca tccattgagg gtaccatcaa cggcgtaccc ttcaattgta
     1321 agggggcaac ctgtccggta aagttgacga tcacatccga cattggggtg acggtcgaat
     1381 tttgggctaa ttctacgttc ggcgactcca gtccacgcta caccgcccag gtgagggtta
     1441 tcgacgccgg agtttccctg gtccctggtg gcgggggatt ccacattgac gtactcagca
     1501 gtcaatggaa aggcggtccg ctcgctagtt gctcgcgtac ctgggaaact tttcctccgg
     1561 tgggcggcac cgtagactgg ttgaggagcc ccaatcaacc cgctttactg gtatccgatg
     1621 aggcttatta ctacctagcc gggagattga tcgcccaagg ggttgttgat gcatctaaat
     1681 gcccatcatt gggactgcta cccaatggat atgccaacgc ttgtggattg gaagtagctg
     1741 ccccaattgt tatggcctgg caaaaccagt tcgacgcacg catcatccag gtatccaaag
     1801 aaaccggcgt acccgctcaa ttactcaaaa acctgtttgc gcaggagagc caattttggc
     1861 ctgggatgtt caaagttccg tttgagtttg gcttggggca aattacagat aaaggagcag
     1921 acaccatact gctttggaac acagatttct acgagacatt ttgtccgttg gtcctgagta
     1981 agaaagcctg tgacaatggt tacctgaagc tgcaagttga tgatcaggct atgttacgtg
     2041 gagcactggc gcagcaggca aagtccgact gcccgctttg tcctgcgggt gtggatttga
     2101 ccagcgcgaa ctccagcatc tccctgttcg ccaacacgct gctatcgagc tgcgatcagg
     2161 tagctcaaat tgtccataat gcgacgggcg aaaagccagg agcggtgagc agctatgaag
     2221 atttgtggcg tttcaccatc gccaattacc acgccggccc aggttgcctg tcgtatgcca
     2281 tttatgctac ctgggctgca aacgggcagg aactactctg ggatgaggta accacccagt
     2341 tcaccgaggc ctgcaaaggc gtgataccat acgtggataa aattgcccgc tgacggttag
     2401 accagagctt ccaccagctc taaaaactca tcccactcct cctgaaagaa gtgcacagta
     2461 acactggcga gttccaagtg gtaagttgtt tcgccatctg gctcttttgc gcgccaggcg
     2521 acatagttgt cagtctctgc gatgacatca gtggatggtt cgggaagatc tttttctttt
     2581 gccacatcac cctccaattc tttcttccat aatcattagg cgctcgttgt tcatggtgga
     2641 gcgcttttct ttggggatta cctcactcca atgcactcgc ccagcactgt aataggttcg
     2701 ataacaccag atttgggcgt gttacctatt ttatccttac ggagctggca aaacatacca
     2761 cggactgaca aagcctccca ggtaacgcac ttcaaaatgt agatgagctc cggttgaatt
     2821 accagtgctt cctgagtagc cgatgatttg accttgaccc acgctacgcc cgcaggaagc
     2881 gctggcgttg ctcagatggg catatacact ctggtaaccg ttgccatggt caaccatgac
     2941 catgttgccg tatccaccgg ttgcccagcc agagaatact accacaccgc tatcagccgc
     3001 atagatcggg gcaccctctc cggctgcaat atcaatccct aggtgaccag accagtagtc
     3061 attgcccgag agagaatgat tgccagcagg ccatataaac cctccactgc cgaatgctcc
     3121 ctcgtagcca ccttcgcaag ctccagcgcc atacacggat ttggataccc ctgccgcacc
     3181 gcgcgggatg accgggataa tccactggcg gaactcgcga tggcctcctg gcaccattac
     3241 ccattcacca ggcgatttgg tcggttcagt caggtccaaa ttattgcctg accaatttaa
     3301 tatatcatca atttttgctt caaaa
//
LOCUS       DBNHOH010000768         2255 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1650095, whole genome shotgun sequence.
ACCESSION   DBNHOH010000768 DBNHOH010000000
VERSION     DBNHOH010000768.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1650095"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="AC16V7_13785"
     CDS             <1..305
                     /locus_tag="AC16V7_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR6258239.1"
                     /translation="DILQELKVRHPSIGDVRGKGLMVGIEFVLDHQTKVPAEKLRDRV
                     VDLAFSMGLLMLGCGKSVVRISPPLSISRSEVDEAMEIFEQAVTLAEREPEILHAA"
     gene            295..1860
                     /locus_tag="AC16V7_13790"
     CDS             295..1860
                     /locus_tag="AC16V7_13790"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6258240.1"
                     /translation="MLPDVRVRQRDYLLEISRAITQELNLDKLLTRILDISAEMLAGQ
                     AGLIALRSERGGWMVAASHGIPPAFLRYLDPLLAAVPDHEDPARFELPEINRLLQNLT
                     RMASLGLLTGVGLPMIVRKQVIGVVFIFRNYKGVFSANDRALLESFADQAAIAVQNAQ
                     LYTQVSREKQRMDALLDSAADGILIIGADHTIERCNPAFARMIGRSMDSIRKRSHEEV
                     IRWKRKDGLTLEQAEAGGWPLTPNATLYIEGDLQREDGLPLPVGITYAPLVNPEGNLV
                     NIIATIRDITRFREAEELKSTFISVISHELKTPVALIKGYVSTLRRDDASWDRKVVQD
                     SLEVIEEEADRLTELIENLLDASRLQAGGLVISTTDVALNKIAERIAERFRTQSSLHT
                     IGVDFPPDFPVVLADEERISQVISNLISNAIKYSPEGGEVRISGQVLPDHVIVCVSDQ
                     GPGIAPDDIPHVFDRFYRSSIAVRTTKGAGLGLYLARAVIEVHGGRIWVDPKPGDGTR
                     VCFSIPRTYPVNY"
     gene            1935..>2255
                     /locus_tag="AC16V7_13795"
     CDS             1935..>2255
                     /locus_tag="AC16V7_13795"
                     /inference="COORDINATES: protein motif:HMM:NF025711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HIR6258241.1"
                     /translation="MAKTRINCPNCRQPIVADIEQLFDVGVEPSAKQSLLSGLTNVAQ
                     CPHCGYQGSVATPLVYHDPEKELLLTFFPPEMGLTRDEQERLIGGLINKVVNNLPQEK
                     RKGYL"
BASE COUNT          550 a          536 c          592 g          577 t
ORIGIN      
        1 tggatattct gcaagagtta aaagttcgcc acccaagtat tggcgatgtg cgtggcaagg
       61 gtttgatggt cggcattgaa tttgtcctgg atcaccagac gaaagtgccg gctgaaaagt
      121 tgcgcgacag agtggtcgat ttggctttta gcatggggct gctcatgctt ggttgtggga
      181 aaagtgttgt tcgcatttcg ccaccgctct ctatctcacg cagtgaggtg gatgaagcca
      241 tggaaatttt tgaacaggca gtaaccttag ccgagaggga gccggagatt ttgcatgctg
      301 cctgacgttc gtgtacgcca gcgagattat ctgcttgaga tatcgcgcgc aattacacaa
      361 gagctcaact tagataagct attgacgcgc attctggata tatctgcaga gatgctggca
      421 ggccaggcag gcttaattgc attacgttct gagcgggggg gatggatggt tgccgcctcc
      481 cacggtatcc ctcccgcttt tttacgctat ctcgacccgc ttcttgcggc agttcctgat
      541 catgaagacc cggctcgttt tgagctcccg gagatcaatc gtcttttgca gaacttaacc
      601 cggatggcta gcttaggact cctgaccggg gtcggtctcc cgatgattgt ccgcaagcag
      661 gtcattgggg tagtattcat ttttcgcaat tataagggtg tattctcagc caacgatcgt
      721 gctcttttag agagttttgc agatcaagct gctatcgcag tacagaatgc ccagctttac
      781 actcaggtta gccgggaaaa acaacgcatg gacgccctac tagattccgc tgcagatggg
      841 atcttaatca ttggagcgga ccataccatc gaacgttgta acccagcctt cgctcgaatg
      901 attggtcgct cgatggatag catccgcaag cgctcgcatg aagaagtaat ccgttggaag
      961 cgaaaagacg gattaaccct ggagcaggct gaagcgggag ggtggcctct aaccccaaac
     1021 gctaccttgt acatagaggg cgacctgcag cgcgaagatg ggttgccctt gccagtgggg
     1081 atcacttatg cgccactggt aaaccccgaa ggtaatctgg ttaatattat cgctacgatc
     1141 cgagatatca cccggtttcg tgaggccgaa gaacttaaaa gcactttcat ctcagtgatc
     1201 agtcacgagt tgaaaacacc tgtagctctc attaaaggtt atgtcagcac gttgcgtcgc
     1261 gatgacgcct cctgggatcg caaagtggtc caggatagct tggaggtaat tgaggaagaa
     1321 gctgaccggt taaccgaact gattgaaaat ttgcttgacg cttcccgatt gcaagctggt
     1381 ggattggtca tcagcacaac ggatgtagct ttgaataaga tcgccgagcg tattgccgaa
     1441 cgatttcgca cccagagcag cctgcatact atcggcgtgg atttcccacc ggatttccca
     1501 gttgtcctgg cggatgaaga acgcatttct caggtgatct cgaacttaat ctcaaatgcc
     1561 atcaaatatt cacccgaggg tggagaggtg cgtatttctg gtcaagttct cccagatcat
     1621 gtgattgtat gtgtcagcga tcagggtcca ggcatcgccc ccgacgatat accccacgtg
     1681 tttgatcgct tttaccggtc ttcaatcgcc gttcggacga ccaagggcgc gggattgggc
     1741 ttatacttgg ctcgtgccgt tatcgaagtc catggtgggc gtatatgggt tgaccccaaa
     1801 ccaggtgatg gaacacgggt gtgtttctca ataccccgga cgtaccctgt gaattattaa
     1861 agcacgcacg caattgatgg ataatctcat gccaattgcg taacttctaa gttatatgtt
     1921 ttctggagat ataaatggca aaaacccgca ttaattgtcc aaactgccgt caaccaatcg
     1981 tcgctgatat tgaacagcta tttgatgttg gtgtagaacc ctctgcaaag caaagcctgc
     2041 tttcaggcct aaccaatgta gctcaatgtc cccattgtgg ttatcaaggc agcgtggcaa
     2101 ccccactggt ctatcatgat ccagaaaagg aattattgct caccttcttt ccccctgaga
     2161 tgggcctaac tcgcgatgag caggagcgat taattggggg attgatcaat aaggtagtca
     2221 acaatctacc ccaggagaag cggaaaggat atttg
//
LOCUS       DBNHOH010000769         2730 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_304203, whole genome shotgun sequence.
ACCESSION   DBNHOH010000769 DBNHOH010000000
VERSION     DBNHOH010000769.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_304203"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..569)
                     /locus_tag="AC16V7_13800"
     CDS             complement(15..569)
                     /locus_tag="AC16V7_13800"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR6258242.1"
                     /translation="MRPGRPQLLALALIGAGLVILGVAVWVLLPSLTAASQEEISAIP
                     VQVDYAAPDLSLTDLAGNPVSLNDYRGSVVLVNNWATWCPPCKAEMPALQAYYEAHQG
                     QNFTIIAIEAGQPASEVADFVRRYGLTFPVWTDPTLKATVAFRNPGLPSSYVIDASGT
                     VRLAWAGAINLDKLEEHVTPLLEE"
     gene            complement(566..1234)
                     /gene="pdo"
                     /locus_tag="AC16V7_13805"
     CDS             complement(566..1234)
                     /gene="pdo"
                     /locus_tag="AC16V7_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_394797931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(788..790),aa:Sec)
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HIR6258243.1"
                     /translation="MTTKLLNETVAEQVRELFDQLQEPVEVLFFGQEAGCEYCEDTLQ
                     LIQEVVALSDKLGLQVFDLDKDAAVAEQYKVDKAPGMVIAGRDGDQILDYGIRLAGIP
                     AGHEFSSLVHDMILVSGRDSGLDSRTRAFLAGLEQPVLLQVFVTPTUPYCPQAVVLAH
                     RMALESPMVEAEMIEAMEFPELSMRHGVSGVPQTTINDGAGNVVGAVPEPHLVIEIQQ
                     ALAG"
     gene            complement(1371..1577)
                     /locus_tag="AC16V7_13810"
     CDS             complement(1371..1577)
                     /locus_tag="AC16V7_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258244.1"
                     /translation="MNHSIAQPPSEIDVSMPPLPGENTLIMLSKAPRPGDLCPHCGQE
                     QLDYDGTLNLACPRCGLILGGCFT"
     gene            complement(1585..1848)
                     /locus_tag="AC16V7_13815"
     CDS             complement(1585..1848)
                     /locus_tag="AC16V7_13815"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR6258245.1"
                     /translation="MKPEKCRHLLGSLSDYIDGTLEEQLCTQLERHLADCPDCRIVVD
                     TLNMTVSLYKQSADLTSVPEEVRQRLFRCLNLEDLLGNPKTPF"
     gene            complement(1845..2453)
                     /locus_tag="AC16V7_13820"
     CDS             complement(1845..2453)
                     /locus_tag="AC16V7_13820"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6258246.1"
                     /translation="MNSKKDVAFSLEALKAGDRLEFARLVETYYEMIYRLAIKMLNNP
                     QDAEDVLQETFLKALRGIRKFDGRSSISTWLYRIATNEALMALRRQKPNLISTDEPLE
                     TEEGEQEPIQIVDWCCLPEPELMSGEAQGYLEQAISRLSPNLRAVFVLRDIEGLSTQE
                     TGEVLNLSETAVKTRLSRARLRLREDLSGYFGEKLFEGKRVE"
BASE COUNT          581 a          784 c          729 g          636 t
ORIGIN      
        1 ttttgcttcc aagtctactc ctccaacaaa ggtgtcacat gctcttcgag cttgtccagg
       61 ttgatggccc ccgcccaggc aagccgcacc gtaccgctgg cgtcgatcac atatgagcta
      121 ggcagcccgg gattgcggaa tgcgaccgtg gctttcaggg ttgggtcggt ccaaaccggg
      181 aaggttagcc cgtagcggcg cacaaagtcc gctacctcgg aggccggctg ccctgcctcg
      241 atggcaataa tggtgaagtt ctgcccctgg tgggcttcgt aataggcttg tagggctggc
      301 atttctgcct tgcacggtgg gcaccaggtg gcccagttat tgaccagcac cactgaccct
      361 cgataatcat ttaaggagac agggttgcca gccaggtctg tcaggctcag gtccggggcg
      421 gcataatcca cctgcacagg gatggcggag atctcctcct gactagcagc cgtcaggctg
      481 ggcagcagca cccaaactgc cacccctaat atcaccagcc ccgcgccgat cagcgccagc
      541 gccaaaagtt gcgggcgtcc aggtctcatc cagctaaggc ctgttggatt tcgatcacca
      601 ggtgcggttc gggtacggcg ccaaccacgt tgcccgctcc atcgttgatg gtggtctgcg
      661 gcaccccgct cacaccgtgc cgcatggata attcagggaa ttccatcgcc tcgatcatct
      721 ctgcctcgac cattgggctc tccagcgcca tgcggtgagc aagcaccacg gcttgcgggc
      781 agtacggtca ggtaggggtg acgaagactt gcagcagcac cggctgctcc aacccggcca
      841 ggaaggcgcg cgtcctggag tctaggcctg aatcgcgtcc agaaaccagg atcatatcgt
      901 gtaccaggga gctaaactca tgaccggcgg gaataccggc taaacggatg ccataatcca
      961 ggatctggtc gccgtcgcga ccggcgatga ccatcccggg ggctttatcg accttgtatt
     1021 gctctgcgac cgccgcgtcc ttatccagat cgaaaacttg caggcccagc ttatccgata
     1081 gggctaccac ctcctgtatc agttgcaggg tatcctcaca gtactcgcag cccgcttcct
     1141 gtccgaagaa aagcacctct accggttctt gtaactggtc gaatagctca cgcacctgct
     1201 ctgcgacggt ctcgtttaat agcttggttg tcatttcgat aattcactcc tgtttcagag
     1261 attacacggt tgattattat tagtaaggag cactatgtgc ccacaccttt gttcaactgt
     1321 gtaaactctc tacttcttgt ttgcacccca acggggcgag aaacaaatca tcaagtaaaa
     1381 cacccgccca ggatcaatcc gcagcgtggg catgccagat tgagtgtgcc gtcataatcc
     1441 agctgctcct gtccgcagtg cgggcataag tcaccagggc gcggggcttt agaaagcata
     1501 atcaatgtgt tttcccccgg taatggcggc attgaaacgt caatttcgct gggtggctgg
     1561 gcaatcgagt ggttcattac gtctctaaaa tggtgtcttt ggatttccca acaaatcttc
     1621 caggttcagg cagcggaaaa ggcgctggcg cacctcttct ggcactgaag tcaggtcagc
     1681 agattgcttg tacagggaga cggtcatatt cagcgtatcc accacaatgc ggcagtctgg
     1741 gcaatccgcc aggtggcgtt caagctgcgt gcacagttgt tcttccaggg tgccgtctat
     1801 gtagtccgag agcgacccca agagatggcg gcatttttca ggtttcattc cacacgcttc
     1861 ccttcaaaca gcttttcacc aaaatatcct gataagtcct cgcgcaagcg caggcgggcg
     1921 cgtgacaggc gggttttcac ggctgtctcg ctcaggttca gcacttctcc agtctcctgc
     1981 gtggacaggc cttcgatgtc gcgcagcacg aatacggctc gcaaattggg ggacaagcga
     2041 ctgattgcct gttccaggta gccttgcgcc tctcctgaca tcaattcggg ctcgggcaga
     2101 caacaccagt ccacaatctg aatgggttcc tgttcgcctt cctccgtctc caatggctcg
     2161 tcggttgaga tgaggttcgg cttctgccgg cgcagtgcca tcagtgcttc gttggtggct
     2221 attcggtaga gccaggtgga tatactcgag cgcccgtcaa atttccggat gcctcgcaga
     2281 gctttgagaa atgtctcctg gagcacatct tcagcatcct gggggttgtt gagcatctta
     2341 atcgctagac ggtaaatcat ctcgtaatat gtctctacca gccgggcaaa ttccaaccgg
     2401 tctccggcct tcagcgcctc cagcgagaac gcaacatctt ttttactatt cattagatgc
     2461 aatcctatcg aaaaagttac ttcacaccca ggtgtgaggg cgctgtcaag gggcttagcc
     2521 ggcttgtgcg ctgtctcccc tccctcccta gaccacagac cctgctcgca ataggatggc
     2581 ggctggtcta atgcaagcag ggcagcaaga ggtctaggta agagatacga ccaaattatt
     2641 ttatcatatt gacggtctca gacttgcgtg ctataaagca aggcacatga gcattatctc
     2701 cttaatgacc gactttggta ttaaagatgg
//
LOCUS       DBNHOH010000770         4055 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1586116, whole genome shotgun sequence.
ACCESSION   DBNHOH010000770 DBNHOH010000000
VERSION     DBNHOH010000770.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1586116"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2890
                     /locus_tag="AC16V7_13825"
     CDS             <1..2890
                     /locus_tag="AC16V7_13825"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR6258247.1"
                     /translation="IRSESVPGLSSVLVYFEPGTDLYRARQMVAERLAQAAVGLPHVS
                     KPPTMIQPLSSASRFMIVGLSSKELSLIEMSVLARWTIAPRLMGVPGVAHVAIWGNRD
                     RQLQVLVDPEELQERGVSLQQVVETTGNALWVSSLSYLEASTPGTGGFIDTPQQRLGI
                     WHVLPISSPEELAQVPVKGTAWRLGDVAEVVEDHQPLIGDAVVNDSPNLLLVIQKLPG
                     VNTLEVTQGLDEALDALRPGFAAIDFDATLFRPATFIEASIANLTRTLVISAFLIVLI
                     IGVFLYGWRTALVSLVAIPLPLVAAVLVLYLRGATFNAMVLAGLVIALGIIVDDAIVD
                     IEHIMRRLRQDHKEGKPKRIEKIILEASIDVRGTIFFATLISLLAVLPVFFMEGVSGA
                     LFRSLAVSYALAMIASLAVALIVTPALSVMLLSNAPLEDRQSPLIPWLQRSYERALER
                     NTQRPQLAYALVAVLIVAGIVLLPFLGGNQLLPSLREPYLSIRLDAPPGTSHPRMSQI
                     VADASSELRAIPGVSNVSAHVGRAVFGDQVVGINSAELWVSIKPDADYDATVAAVKRQ
                     VDGYTGLDHEVSTYMQQALGEPLSSTDDIFTVRVFGEDHDILRDQAQKLQQTVAGING
                     VVDPHVVLPVEEDSVEIEVDLASAQNYGVKPGDVRRAAATLLSGIQVGSLFEQQKVFD
                     VMVWGAPEVRDSLEDIPELLIDTPRGGHVRLGDVADVRVVSSPTVIKRIAVSPYLDIN
                     FSVQGRDVNAVLGDVEHSLQNFAFPLEYHAELQGDYAARQAIQQRILIAGIVALIGIF
                     LLLQAASESWRLALAILLSLPAALVGGILAAFLSGGSFSLISYFGLFAILGIAARNTV
                     MLIKHYHSLEQEGEEFGLGQVMRGSRERVAPMVMTALTTGLALMPFVLFGNIPGHEIV
                     RPLAIVVIGGLVTSTLLNLFVLPVLYLRFGASREPDLQFLSVPSASEA"
     gene            2955..3938
                     /locus_tag="AC16V7_13830"
     CDS             2955..3938
                     /locus_tag="AC16V7_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6258248.1"
                     /translation="MEDSNNHQVLLAENLRKSYGPRLALRGLSFSLKAGRILGFLGPN
                     GAGKTTAIRILTTILEPDSGHFVVDGISSEYPDKIRRRIGVLPENLGFPRQMTGLEYL
                     THFGQLYGVSTADARSNSLRLLEDVGLKQRAKSLIGTYSHGMRQRIGIARALVNDPVV
                     VFLDEPTLGLDPRGQQELLTLIKRIVQERNVGVILCSHALSEIEEICDDVVILSSGQI
                     IASGPVADVIGRAQRNVIRVRVPISSVDQATQKLEALPNIIKVASSNGKTGWLVMERV
                     GLTDTSSTPKPDANNKILEALIKAKIPVLGYEVEGGRLQDVFLHLTEEVIK"
BASE COUNT          863 a         1034 c         1145 g         1013 t
ORIGIN      
        1 catccgctcg gagtctgtgc caggactttc atcggtgctt gtctacttcg aacccgggac
       61 cgatctctac cgtgcacggc agatggtggc agagcgtctt gcccaggcag ccgtcggcct
      121 gccccacgtc tcaaaacctc caaccatgat tcagcccttg tcctcggcaa gccggttcat
      181 gattgtgggg ctttcatcaa aggaactatc cttgatcgag atgtcggtgc ttgcccgttg
      241 gacaatcgct ccgcgcttga tgggtgtgcc cggagttgcc catgtcgcta tttggggcaa
      301 ccgggataga caactgcagg tgctggtcga tccggaagag ttacaggaaa ggggtgtatc
      361 gctccagcaa gtggtagaga cgaccggaaa tgcgctttgg gtatcctcgt tgagctactt
      421 agaggcgtcg acgcctggga ctggaggctt tattgatacc ccccaacaaa ggctcgggat
      481 ctggcacgtc ttgcccatct cctcgccaga ggaattggct caagtgccag tcaaaggcac
      541 cgcttggcgc ctaggtgacg tggccgaagt agtagaagat caccagccgt tgatcgggga
      601 tgcagttgtc aatgacagcc ctaatctact gctagttatc caaaaacttc ctggggtcaa
      661 caccctggag gtgacacaag ggttggatga agcgttagat gcgttgaggc ccgggtttgc
      721 cgcgattgat tttgacgcta ccctcttccg tccagcgacc ttcattgagg cgtcgatcgc
      781 taatctcacc cggacgttgg tcatctctgc gttcctcatc gttttgataa tcggcgtatt
      841 cctatacggt tggcgaacag cgcttgtcag cctggtggcg atcccattgc cactggtagc
      901 cgctgtgctc gtcctgtacc tgcggggggc tacgtttaat gcgatggttt tggcgggctt
      961 ggtaatcgcc ttaggcatca ttgtcgacga tgccatcgtc gatatcgaac acatcatgcg
     1021 gcgcctacgc caagaccata aggagggcaa acccaagaga atcgagaaga ttatcctgga
     1081 agcctcgatc gatgtgcgcg gtaccatctt ctttgccact ctgatcagcc tgctggctgt
     1141 gctaccggtc ttctttatgg aaggagtatc cggcgcattg ttccggtcat tagctgtctc
     1201 ctacgctttg gcgatgatcg cctcgttagc tgtggcactg atcgttacgc cagcgctgag
     1261 cgtaatgctg ctttctaatg cgccgctcga agatcgccaa tcacccctca tcccgtggtt
     1321 gcagagaagc tacgaacgtg ctctggagcg taacacccaa agaccccagc tggcgtatgc
     1381 gcttgtcgcc gttctgattg ttgctggtat tgtgttgctg ccattcctag gggggaatca
     1441 actgcttcct tcgctccggg agccatacct ttcaattcgt ctggatgctc caccaggcac
     1501 atctcatccg agaatgagcc agattgttgc tgatgcgagc agtgagctgc gcgccattcc
     1561 cggggtcagt aatgtcagtg ctcacgttgg acgggcagtg ttcggagatc aggtggtcgg
     1621 aattaactcc gctgagttgt gggtcagcat caaacctgac gctgactatg atgcgacggt
     1681 agccgctgtt aagcgccagg tggatgggta caccggactt gatcatgagg tgagcaccta
     1741 tatgcagcaa gcgctgggtg agccactgtc gagcacagat gatatcttca ccgtgcgcgt
     1801 ctttggcgag gaccacgata tcctacgtga ccaggcccag aaactgcagc aaactgtggc
     1861 cgggatcaac ggcgtcgttg atccgcatgt agtgctcccc gttgaggaag actcagtgga
     1921 gatcgaggtc gatcttgcct cggctcaaaa ttatggtgtc aaaccaggcg atgttcgccg
     1981 tgcggcggcc accttgctct ccggtattca ggtcggcagt cttttcgagc aacagaaagt
     2041 gtttgatgtg atggtatggg gcgcgcccga ggtgcgtgac agcctggaag atatccctga
     2101 gttattgatt gatacacctc gtggaggtca tgtgcgattg ggggatgtag ccgatgtgcg
     2161 cgttgtgtca tctccgaccg tcatcaagcg tattgcggtc tcaccttacc ttgatattaa
     2221 ctttagcgtt cagggaaggg acgtcaacgc cgttttgggg gatgtcgagc attctctgca
     2281 aaacttcgcc ttcccgttgg agtaccatgc cgagctgcaa ggtgattatg cggcacggca
     2341 ggcgatacaa cagcgcattc tcatcgccgg gattgtggca ctgatcggga ttttcttact
     2401 cctgcaggct gcctccgaaa gctggcgcct ggctttggcg atcttactct ccttgccggc
     2461 ggcgctggtg ggcggaatat tggcagcgtt cctgagtggt ggctcgttct cgctaatttc
     2521 atacttcggg ctgtttgcga tattgggaat tgcggcgcgt aataccgtca tgctaatcaa
     2581 acattaccat tctcttgagc aggagggtga agagtttgga ttagggcaag taatgcgcgg
     2641 ttctcgtgaa cgcgtcgcac cgatggtgat gacggcgtta accacaggcc tggctcttat
     2701 gccgttcgtg ctcttcggca atatccctgg tcacgagatt gtacgcccgc tggctatcgt
     2761 tgttattgga ggattagtca cttcgacgtt gctcaatctt ttcgtcctgc ctgtcctgta
     2821 tttacgcttc ggggctagcc gtgaacctga cctccaattc ctttcggttc catctgccag
     2881 tgaagcataa acctgaagac ttgttaatgg gtctcgagac gagcaaaatt tagcaaattc
     2941 caggagatta attgatggaa gacagcaaca atcaccaagt tttactggca gaaaacctgc
     3001 gaaaatctta cggacccagg ctggcactcc gagggctatc cttctcattg aaggctggac
     3061 gtatcctggg cttcctgggc ccaaacggag cgggtaaaac gaccgcgatc cgcatcctta
     3121 ccaccattct tgagcccgat tcaggccatt ttgtcgtgga tggaatcagt tccgagtatc
     3181 ctgacaaaat ccggcgcagg atcggcgtcc tgcctgagaa cctgggattt cccaggcaaa
     3241 tgacgggctt agagtacctg acacacttcg gtcagctcta cggggtatca acggctgatg
     3301 ccagatccaa tagcttacgc cttctcgaag atgtgggatt gaagcagagg gcaaaatccc
     3361 tgattggcac atacagtcac gggatgagac aaaggatagg catcgcgcgc gcgctggtca
     3421 atgaccctgt ggtcgttttt ctagacgaac ccactctcgg attggatccg cgcggtcaac
     3481 aggagttatt gactctcatc aagcgcattg tgcaagagcg aaatgtgggt gtgattttgt
     3541 gcagccatgc tttgtccgag attgaagaga tttgtgacga tgtagtcatc ttgagttcag
     3601 gccagattat tgcctcggga cctgtggcgg atgtcattgg aagggctcag cgtaatgtca
     3661 tccgcgtccg cgtcccgata tcatcggtcg accaggctac acagaaactg gaggcattgc
     3721 cgaatattat caaagtagct tcctcaaacg gtaagacggg ttggttagtg atggagcgcg
     3781 tgggtttgac ggatacatcc tccaccccaa aacctgatgc caataacaaa atactagaag
     3841 cccttatcaa agcaaaaatt cctgttttgg ggtacgaggt tgagggtggt cggttgcagg
     3901 atgttttcct acacttaact gaagaggtaa ttaaatgagc gctaaacaag aagtgttcgt
     3961 gactagtgtc aaaccattga gctcagcgaa gcctgcatgg tggctgtttt tcgtcaggga
     4021 actcgcagac ctgtggatcg gcggtaaagc tctca
//
LOCUS       DBNHOH010000771         3497 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_496703, whole genome shotgun sequence.
ACCESSION   DBNHOH010000771 DBNHOH010000000
VERSION     DBNHOH010000771.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_496703"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="AC16V7_13835"
     CDS             <1..455
                     /locus_tag="AC16V7_13835"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR6258249.1"
                     /translation="IAPPDGPPTFEFKPEERGKLPISPENLKVIQDAMRGVVVSTKPY
                     GTAWHRFTGLDINVAGKTGTAESGFGDPHAWFAGYTFEDRPNQPDIAAVVIVENIGEG
                     SDYAAPIFRRVVELYFYDRPAKLYPWESGFYVTRTPTPEFVGEETPEP"
     gene            530..1432
                     /gene="rsgA"
                     /locus_tag="AC16V7_13840"
     CDS             530..1432
                     /gene="rsgA"
                     /locus_tag="AC16V7_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIR6258250.1"
                     /translation="MLSGLIIRAQSGFYTVETEKGLVVCRLRGLLKRGPRRGDVAAVG
                     DWVDLTLLDSETGVIQAIKPRLRMLSRLAPNPQGVYHQIIIANPDQAVFVFSCTRPEP
                     RFGMLDRFLVIAEKQQIPSVIVANKIDLLEREAARDLFGHYRPLGYPVFYTSARTGEG
                     VEALRERLSSKISVFAGPSGAGKSSLLNQLMPGLELKSREVSRSTRKGRHTTVVREMF
                     PLPGGGYVADTPGLKALALWDIQPEELDGYFPELRQRVSGCAFSDCTHTHEPGCDVIS
                     AVEAGEVHPERYSSYLKMRLGEEE"
     gene            1613..2644
                     /locus_tag="AC16V7_13845"
     CDS             1613..2644
                     /locus_tag="AC16V7_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6258251.1"
                     /translation="MKEKKPLMVSGNGQPTLLKVENLITRFYTPEGVVHAVNGVSFDL
                     AEGETLGVVGESGCGKSVTMLSVLQLIARPPGKIESGHAWFDGKDLLYLPTDQIRDVR
                     GSRISMIFQDPMTSLNPVLTIERQISESLMLHLRMTKQQARERVIELLEMVGIPEAET
                     HLNDYPHQFSGGMRQRVMIAIALACNPQILIADEPTTALDVTIQAQIVDLVKRLRDDL
                     NMTVVWITHDLGVVARLAQRVIVMYAGYIIEEAPVKELYANPTHPYSIGLLGSLPSLT
                     TDDRKRLTSIPGLPPVLLEKPVGCPFAVRCDHAFDRCKQENPPLMSIGSLHRVACWWD
                     VQEGRPRNA"
     gene            2637..>3497
                     /locus_tag="AC16V7_13850"
     CDS             2637..>3497
                     /locus_tag="AC16V7_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6258252.1"
                     /translation="MPEDQVILKVDKLVKHFPIYRGVFQRQVGAVHAVDSISFDVRRG
                     ETFGLVGESGCGKTTAGRTIIQLYKPTSGHIYYNGTDLTTLKGGDMRRMRKSIQMIFQ
                     DPYASLNPRISIANTLSEPILIHGLARGAQARQRVDELLNIVQLNPGFASRFPHEFSG
                     GQRQRIGIARALALNPELIICDEPISALDVSIQAQIINLLEDLQKQFGLTYLFIAHDL
                     SVVRHISSRVAVMYLGIIAELATVKELYNNPLHPYTQALLSAVPVPNPIIEEKRQRII
                     LEGDVPSPVNP"
BASE COUNT          780 a          930 c          984 g          803 t
ORIGIN      
        1 gcattgcgcc acccgatgga cctcccacct tcgagttcaa gcccgaggag cgcggcaagc
       61 tgcccatcag cccggagaac ctaaaggtga tccaggatgc catgcgcggg gtggtggtga
      121 gcacaaagcc ctacggcact gcctggcacc gcttcacggg gttggatatt aatgtggctg
      181 ggaaaaccgg cactgctgaa tcaggctttg gggatccgca tgcctggttc gcaggctata
      241 ccttcgagga tcggccaaat caacctgata tcgctgcggt ggtgattgtt gagaacatcg
      301 gtgagggctc ggattacgcc gcgccgatct tccggcgggt ggtggagctt tatttctatg
      361 ataggccagc taagctctac ccgtgggaat cgggtttcta tgtgactcgc acccctacac
      421 cagagttcgt gggggaagag acgccagaac cttaatgggt agactttgac agaaagtccg
      481 gttgtggctc ttcaatgatt gcttaccaag tggaattcaa aactctcata tgctatctgg
      541 actgatcatc cgagcgcagt cgggattcta cactgtcgag actgaaaagg gattagtcgt
      601 ctgccgcctg cggggtcttt tgaaacgcgg gccgcgccgg ggggatgtgg ccgcggtggg
      661 cgattgggtt gatttaacgc tgctggatag cgaaaccggc gtgatccaag caataaagcc
      721 gcgcttgcgt atgctctccc gtctggcacc caatccgcag ggggtctatc accagatcat
      781 cattgccaat cccgatcagg cggtgtttgt gttctcttgc acgcgccccg agccgcgctt
      841 tggcatgctg gatcgcttcc tggttatcgc tgagaaacag cagatcccct cggtgattgt
      901 cgccaacaag atcgatctgc ttgagcggga ggctgcccgt gatttgtttg gtcattatcg
      961 accgctgggt tacccggtgt tttatacgtc cgcccgcact ggagaagggg tggaagcgct
     1021 gcgggagcgg ttatctagca agatctcggt gttcgctggt ccatcgggtg caggcaaatc
     1081 cagcctgttg aaccagttga tgcctggtct ggagttgaaa tcccgtgagg tgagccgctc
     1141 cacccgcaag ggtcggcaca ccaccgtggt gcgtgagatg ttccctttgc ccggtggtgg
     1201 ctacgtggct gatacgcccg gtctgaaggc tttggctttg tgggacattc agcctgaaga
     1261 gttggatggc tatttcccag aattgcgcca gcgcgtctcg ggttgcgcct tcagcgactg
     1321 cacccatacc catgagccgg gctgtgatgt aatctccgcg gtggaagctg gcgaagttca
     1381 ccctgaaaga tatagttcgt atctcaaaat gcggctggga gaagaggaat aaagaaccaa
     1441 tataatacat taactgccag aaaggttaca ttaatcccta ctattacaga ttgttcgaag
     1501 gtacaatccc cctgccgcgt atccaactgc gtaagtccta aaaatattaa gccagaatca
     1561 ttgaacctta agcgaataca atgagttaag tatatagagg aaatacgtat caatgaagga
     1621 gaagaagcct cttatggttt caggcaacgg acaacctacc ctcttgaaag tggaaaactt
     1681 aataacccgt ttttatacac cagaaggtgt ggttcatgca gtgaacggtg tcagttttga
     1741 cctcgccgaa ggggaaactt tgggtgtagt aggcgaaagc gggtgtggaa aaagcgtaac
     1801 catgctctcg gtgcttcaat tgatcgctcg accacctggg aagatcgaga gcgggcatgc
     1861 ctggttcgat ggaaaagact tgctttacct gcccacggac cagattcgcg acgtgcgcgg
     1921 ctcacgcatc tcgatgatct tccaggatcc gatgacctcc ctcaacccgg tcctgactat
     1981 cgagcggcag atctccgagt cgctcatgtt gcacttgcgc atgaccaagc agcaagcgcg
     2041 cgagcgggta atcgaactgc tggagatggt gggcatccca gaagcggaga cacacctgaa
     2101 cgattacccc caccagttct ccggcggcat gcgccagcgg gtgatgatcg ctatagccct
     2161 ggcatgcaac ccgcaaatct tgatcgccga cgagcccacc accgccttgg atgtgaccat
     2221 ccaagcccaa attgtagatc tggtcaagcg cctgcgggat gatctcaata tgacggtggt
     2281 ttggatcacc catgacctgg gagtggtagc ccgactggcg cagcgagtga tcgtgatgta
     2341 cgcgggatac attatcgaag aggcgccggt caaagagttg tatgccaacc ccacgcaccc
     2401 ttattcgatt ggcttgctgg gcagcttacc cagcctgacg accgatgatc gcaagcgttt
     2461 gacctctatt cccggtttgc ctccggtgct gttggaaaag ccggtgggtt gtccctttgc
     2521 ggtacgttgt gaccacgctt tcgatcgttg caagcaagaa aatcctccct taatgagcat
     2581 tggatcattg caccgagttg cgtgttggtg ggatgtccag gaagggaggc cgcgcaatgc
     2641 ctgaagatca agtcatactg aaagttgata agctggtcaa gcacttcccg atctatcggg
     2701 gtgtttttca gcgccaggta ggggctgtgc acgccgtaga tagtatctcc tttgatgtgc
     2761 gccgcgggga aacctttggc ctggtgggcg aatcgggctg cggcaagacc actgcaggcc
     2821 gtacgatcat ccagctttat aaacccacct cggggcatat ctattacaac ggcacggacc
     2881 tgactaccct aaagggtggc gatatgcgcc gcatgcgcaa aagcatacag atgatcttcc
     2941 aggatccgta tgccagcctc aatcctcgca tatccattgc taatacgctc agcgagccaa
     3001 tactcattca cggattagca cgcggagcgc aagctcgcca gcgcgtcgac gaactgttga
     3061 atatcgtgca gttgaacccc ggctttgcca gccgcttccc acatgaattc tccggcggac
     3121 agcgccagcg cattggcatt gcgcgtgccc tggctctaaa tcctgaattg atcatctgtg
     3181 acgagccgat ctccgccctg gatgtttcca ttcaggcgca aattattaac ttgctggaag
     3241 atttacagaa acagtttggc ctcacctact tgtttatcgc tcatgacctg tctgtggtgc
     3301 gccacatcag cagccgtgtg gcggtcatgt atctgggtat tatcgccgag cttgctacgg
     3361 tgaaggagtt gtataacaac ccattgcatc cctacacaca ggcattgctt tccgcggtgc
     3421 cggtgcccaa cccgatcatc gaagaaaagc gccagcgtat tatcctggaa ggcgatgtgc
     3481 ccagcccagt gaacccg
//
LOCUS       DBNHOH010000772         1221 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1522343, whole genome shotgun sequence.
ACCESSION   DBNHOH010000772 DBNHOH010000000
VERSION     DBNHOH010000772.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1522343"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="AC16V7_13855"
     CDS             <1..500
                     /locus_tag="AC16V7_13855"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF042869.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HIR6258253.1"
                     /translation="PIGGHPMCGSEKSGLAYAAPQIFRGATFTFTPLERTSQRAKAVC
                     TQITEVIGARALWLDPFTHDRWTASTSHIPYLVASALAASTPREAAPMVGPGFRSTTR
                     LAASSTRMMRDILATNRQNVLAGLKGLVLHLEEIEKLLAQSDFDALETLLDRGLEQHN
                     ILVGE"
     gene            529..>1221
                     /locus_tag="AC16V7_13860"
     CDS             529..>1221
                     /locus_tag="AC16V7_13860"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR6258254.1"
                     /translation="MKLISTPGNSIHGELTLPGDKSLSHRALLFAALAHGESVIDNFL
                     MAGVTKVMLDALKSLGVDWEFKGSRLIVQGKGLDGLQPPAESIQCGNSATTIRLLTGA
                     LAAAGVPAVLDGSPGLRRRPMKRIVTPLQEMGVPIEDSREGTVPIRLEARLKNQLLRA
                     VDYRLPVSSAQVKTCLLLAALAADGTCILHEPQPSRDHTERMLAMMGVNIQAEGRQVK
                     LTPSQPTQLRPLH"
BASE COUNT          283 a          331 c          343 g          264 t
ORIGIN      
        1 acccaatagg agggcatccg atgtgcggta gcgaaaaatc tggtttggcg tacgctgccc
       61 cgcaaatctt ccgaggtgca acattcacgt tcacaccgtt ggaacgaact agtcagaggg
      121 caaaggcagt atgcacacag ataactgaag tgatcggagc tcgagcgctc tggttagacc
      181 cattcacgca tgaccgctgg acggcttcca ccagccatat cccttacttg gtagccagcg
      241 ccctggcagc gtcgaccccc cgcgaagcag cccccatggt aggtccgggc tttcgcagca
      301 ccacgcgcct ggctgcctcg tccacgcgca tgatgcggga tatcctcgca accaaccgcc
      361 aaaatgtgct ggcaggttta aaaggtttgg tactgcatct ggaggaaatt gagaagctgc
      421 tcgcccaaag cgatttcgat gccttggaga ccttgctaga tcgtggctta gagcagcaca
      481 atattttagt aggggaataa atatccgtca aatttggtat gcaaatccgt gaaattaatc
      541 tcgacgcctg gcaactccat tcatggtgag ctcacgctcc ctggcgataa atctctatcg
      601 catagggcgc tgctatttgc tgccctggct catggggaga gtgtcatcga taatttcctg
      661 atggctggtg tcacaaaggt gatgctggat gcgctgaaat ctctgggtgt ggattgggag
      721 tttaagggaa gccgcctgat tgtccaggga aagggattag acgggcttca acccccagca
      781 gaatccatcc agtgcgggaa ttcagccaca accatccgct tgctgacggg agccttggcc
      841 gcggctggag tacccgcggt gctggacggc tccccaggtt tgcggcgcag gccgatgaag
      901 cggattgtca ccccgttaca agaaatgggc gttccgattg aggacagtcg tgagggtact
      961 gtgccgatac gcctggaagc aagactgaaa aaccaactct tgcgtgctgt ggattatcgt
     1021 ttgccggtat ccagcgctca ggtgaagacc tgcttgttat tggctgcatt agccgcagat
     1081 ggaacctgca tcttgcacga accccagcca tctcgcgatc atacggagcg catgctagcc
     1141 atgatgggag tgaatattca agcagaaggc aggcaggtta agctgacacc ctcccaaccc
     1201 acacagctgc gcccgcttca t
//
LOCUS       DBNHOH010000773         1949 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_368414, whole genome shotgun sequence.
ACCESSION   DBNHOH010000773 DBNHOH010000000
VERSION     DBNHOH010000773.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_368414"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1949
                     /locus_tag="AC16V7_13865"
     CDS             <1..>1949
                     /locus_tag="AC16V7_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258255.1"
                     /translation="DIVAAYTRTIDKGKFAPFGKFINIDVDQEIQVRLDFLDIAIQNQ
                     SDVYLAFDHQPGGVFQWPWGSDTQIAWDTLVLLPASGQIQAWNIHQQDANNTTIRAFR
                     NPVLDTLEVHLDASGLPGSNTGFKLQIMTTPAGEKNAADQLTPFHSKDYPPKPAQVLF
                     AFWNTLPAYTPAQALRRWDGAHTGPLGGRHGLYNLLRTARSHKIPLALLDLKSPSSLS
                     ALDYVDGVQLVQKMAQEGLLILPDTVPILPDITPTTLTPSADFLAQLTKMNRQISQDF
                     GLTTSQFIYSPLTPGLPTNYPVIFTNLPQPIDAIDSPMGIISPLRWRDKVVIPIPEDL
                     LAEQASLDGVSLETRRALAMAAASPTLAMRKDPQIILVLGGDLTYSEWGAPQIARAGF
                     HYLAAHPWIRILGAHDLLSARPSNDLSFTALPTNASGISIAEEAKISQTIEQISQLQP
                     SALSQAAWQALISLYGPISPKPDELSRLRAIYANQLEVLIAAAQWSKKPAHISSCDTD
                     LDLDGQLNCVLASDEIFAVFDETAGSLTYLFVKTSSGFHQVIAPSSQFAVGLSESSGW
                     MLDKGLGADPSIIPGAFNDETQQYQAIFEPPQLTFFNQELTKVYRPIPGGIRVEYRAS
                     TPISTRLPIALDPWVRFGPSWGERYS"
BASE COUNT          477 a          587 c          424 g          461 t
ORIGIN      
        1 gacatcgtcg cggcgtacac tcgcaccatc gataaaggca aattcgcccc ctttggtaaa
       61 ttcattaata tcgacgtaga ccaggagatt caggttcgcc ttgattttct ggacatcgcg
      121 attcaaaacc agagcgacgt ttatttagct ttcgatcacc agccgggggg tgtcttccaa
      181 tggccttggg gtagtgacac gcaaattgct tgggataccc ttgtgctcct gccggcctct
      241 gggcaaatac aggcttggaa catccaccag caagatgcca ataacacaac tatccgcgcc
      301 tttcgaaacc ccgttctcga cacattagag gtgcatctgg atgcttctgg gctacctgga
      361 tcaaacacag ggtttaagtt acagatcatg accacacctg ctggtgagaa gaatgccgcg
      421 gaccaactca ccccattcca ttctaaagat taccccccca aacccgccca ggttttattc
      481 gccttttgga atacattgcc cgcttatacc cctgcccaag cgttgcgtcg ctgggatggc
      541 gcacacactg gcccattagg aggccgccac ggactgtaca atctgttgcg cactgcacgc
      601 agccacaaga ttccgcttgc cctattagat ctaaagtccc ccagctccct ttccgcactg
      661 gattatgtag acggcgttca actggtacaa aaaatggccc aggaaggctt gctcatcctg
      721 ccagacaccg tgccaatttt accggatata actccgacca cactaacacc gtcagcagat
      781 ttcctggcac agctaaccaa aatgaaccgt caaattagcc aggacttcgg cctcacaacc
      841 agtcaattca tctattcccc actaacaccg ggattaccta ctaactatcc ggtgattttt
      901 actaatttgc ctcaaccaat agacgcgatc gactcgccta tggggatcat cagcccgctg
      961 cgctggcgcg ataaagtggt tatccccatt ccagaagact tattagcaga gcaagcctct
     1021 ctagacggtg tgtcattaga gacgcgcaga gcgctggcta tggccgcagc ttcaccaact
     1081 ttggctatgc gaaaagaccc ccaaatcata ttggtcttgg gaggagacct gacctacagt
     1141 gagtggggcg ctcctcaaat cgcacgcgct ggtttccact atctggctgc ccatccgtgg
     1201 atacgtatcc tgggcgccca cgacctactc agcgcacgcc cgtcaaatga cctctccttt
     1261 acggctctcc ccaccaacgc ttcaggtatc tcaattgcag aagaagcaaa aatatcccaa
     1321 accattgaac aaattagcca gttgcaacct tctgcattga gccaggcagc atggcaggca
     1381 ttaatctcct tgtacgggcc tatctcgcct aaacctgacg agctctcgcg cctgcgagcc
     1441 atctacgcca accagcttga agtccttatc gccgcagcgc aatggtcaaa aaaacccgca
     1501 cacatctcgt cctgcgatac cgacctcgat ctagacggcc agttgaattg cgtcctggct
     1561 tcagacgaaa tattcgccgt atttgatgaa acagcaggct cactgaccta tctctttgtg
     1621 aaaacttcat ctggcttcca tcaagtcatc gccccgtctt ctcaatttgc agttggcctc
     1681 agcgaatctt caggctggat gttagataag ggactgggtg ccgatccatc tattatccct
     1741 ggcgccttta acgatgagac gcagcagtac caggccatat ttgaaccccc tcaattgact
     1801 ttctttaacc aggaactcac caaggtatac cgtccgatac ctggaggcat aagagttgag
     1861 tatcgcgcct ccactccaat ttctactcgc ttacccatag ctctggatcc atgggtgcga
     1921 ttcggcccct cttggggaga gcggtattc
//
LOCUS       DBNHOH010000774         2916 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_304479, whole genome shotgun sequence.
ACCESSION   DBNHOH010000774 DBNHOH010000000
VERSION     DBNHOH010000774.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_304479"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..2225
                     /locus_tag="AC16V7_13870"
     CDS             66..2225
                     /locus_tag="AC16V7_13870"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006825265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0043682 - P-type divalent copper
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HIR6258256.1"
                     /translation="MDHHDMSMKNPNEEHRDNAGHQEMVAHDEHQAHAQETIVQVEME
                     NHAGHEGMDHASAGRQQGHVDHTGHEGMFRQRFWVSLALSIPVLLYSPMIQRWLGFSM
                     PGFPGSQWITPLFSVVVFVYGGIPFLDMARPELRNRRPGMMTLISLAISVAFVYSLGA
                     LFVLEGDTFFWELVTLIDIMLLGHWLEMRSVRQASGALNELAKLMPDTAELIRADGSI
                     TKVQVAKLRHGDMVLVRPGSSIPADGVVEEGDSEVSEAVITGESRPVNKEPGDQVIAG
                     TINGDGSLRVRVTATGDETTLAGIMRLVEQAQQSKSNTQILADKAAGWLFYIALGVAA
                     VTGIAWSVAVGFNVEVIARVATVLVIACPHALGLAIPLVVAITTAIGAKNGILVRDRL
                     ALEQARQIDTVIFDKTGTLTRGEFGVVDIVTSDGWDENQALALAAAIEGDSEHTIARG
                     IRRTAEERGIHLPKVNDFEAIKGRGVKAKHDGQSVYVGGPRLLEMLQFKLPEQIAGFE
                     EQANQKGYSLVNLVVNSEVVAGFALADVIRPESKQAIQKLHDMGVQVAMLTGDSQAVA
                     KSVAEELGIDQYFAEVLPEHKDQKVTELQRQGKRVAMVGDGVNDAPALTRADVGIAIG
                     SGTDVAVESAGIILVKSNPLDVVKIFSLSRASYRKMIQNLLWATGYNVVALPLAAGIL
                     APFGILLSPAVGALFMSLSTVIVAINAQLLRRVDIRG"
     gene            2227..2805
                     /locus_tag="AC16V7_13875"
     CDS             2227..2805
                     /locus_tag="AC16V7_13875"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6258257.1"
                     /translation="MSSSTLSRSSNCGRTRSKFGFYGLLLVAGLLTTACGMFWRFPQF
                     FQSLPASLGGDESVSNGERIYFTATNVDGDWVRYSGGPRFGGMMMGSYLTCAACHGPE
                     GRGGIHTMHMQVMDAPDIRYVALSEEAGEHKDEQQDDHSDAHGEYTLDDFRLAVVEGE
                     HPDGQPLSRDMPRWRMSEKDLADLFEFLKSLP"
BASE COUNT          677 a          667 c          916 g          656 t
ORIGIN      
        1 caatatcgat gcgtaaaaag tctcaatgag cgggcagtgc gaatgttgcc cggaagggta
       61 gtaatatgga ccaccacgat atgagtatga agaatccgaa tgaagaacat cgcgataatg
      121 ctggacacca ggagatggtg gctcacgatg aacatcaagc gcacgctcag gagaccatcg
      181 ttcaggtcga gatggaaaac catgctgggc atgagggcat ggatcacgca tctgcagggc
      241 gccagcaggg tcatgtcgac cacacaggac atgaggggat gttccggcag cggttctggg
      301 tctcgctggc gttgtccatc ccggtgctac tgtatagccc catgatacag agatggcttg
      361 gcttcagcat gccaggtttt cccggtagcc aatggatcac gccgctcttc tcggtggttg
      421 tctttgtgta cggcggcatc ccatttctgg acatggcgag acccgaactg cgcaatcgcc
      481 gcccggggat gatgaccttg atctcgctgg caattagcgt ggctttcgta tacagcctgg
      541 gagccctatt tgtacttgaa ggggatacct tcttttggga attagtgacg ctgatcgata
      601 ttatgttgct cgggcactgg ctggagatgc gcagcgtgcg ccaggcctcc ggcgcattga
      661 acgagctggc caagctgatg cccgacacgg ctgagctcat ccgtgcggac ggcagcatca
      721 caaaggtcca ggtggcgaag ttgcgtcatg gtgacatggt tcttgtgcgc cccgggagca
      781 gtattcccgc tgatggagtt gtggaggagg gtgattcaga ggtaagcgaa gccgtgatca
      841 ctggagaatc caggccggta aataaagaac cgggagacca ggtgattgcc ggaacgatca
      901 atggcgacgg cagtctgcgc gtgcgggtga ccgccacggg cgatgagact accctggcag
      961 ggatcatgcg cttggtggag caggctcagc aaagcaaatc caacacccag atcctggcgg
     1021 ataaagcggc agggtggctg ttttacattg ccctgggtgt agctgctgta accggcatag
     1081 cctggagcgt tgcggttgga tttaatgtgg aagtgatcgc ccgggtggcg accgttttgg
     1141 tgattgcctg ccctcacgcc ttgggtctgg cgatcccgct ggtcgttgcc atcacaaccg
     1201 ccattggcgc taaaaatggc attctggttc gtgataggct ggcgcttgag caggcgcgcc
     1261 agatcgacac agtgatcttt gataaaacgg gtacgctgac gcgtggggag ttcggcgtag
     1321 tcgatattgt gaccagtgat ggctgggatg agaaccaggc gctagcgttg gcagctgcaa
     1381 ttgaaggcga ttctgaacac accattgcac gtggcattcg gcggactgcc gaagaacgtg
     1441 ggatccacct gccaaaggtg aacgacttcg aagccattaa ggggcgtggg gtgaaagcca
     1501 agcatgatgg gcaatctgtc tacgtgggcg ggccgcgatt gttagaaatg ctgcaattca
     1561 agttgccaga gcagatcgct ggatttgagg agcaggccaa tcaaaaaggc tatagcctgg
     1621 ttaacctggt ggtaaatagt gaagtggttg ccggattcgc cctggcagat gtcatccgtc
     1681 cggagagcaa gcaggcaatc cagaaactac atgacatggg cgtgcaggtc gccatgttga
     1741 ccggcgacag ccaagcggtg gcaaagtctg tggcggaaga attgggcata gaccagtact
     1801 ttgccgaagt tttacctgaa cacaaggatc aaaaggtgac cgaattgcag cgacagggca
     1861 agcgggttgc catggtgggc gatggggtga acgatgctcc cgccttgacc cgggctgatg
     1921 tgggtattgc cattggcagc gggacggatg tggcggtgga atcggctggt atcatcctgg
     1981 tgaagagcaa tccgctagat gtggtcaaga ttttctcgct gagccgggcg agctaccgca
     2041 agatgatcca gaacctgctt tgggcgacgg gttataatgt ggttgcattg ccccttgcag
     2101 cgggcatact ggcgcctttt ggcatcttgc tttcaccggc agtaggagcc cttttcatgt
     2161 cattgagcac ggtaatcgtg gcgatcaatg cccagttgct gaggcgggta gatatcagag
     2221 gataaaatga gtagttccac cttgtcccgc tcctcgaact gtggaaggac aagatcaaaa
     2281 ttcggcttct atggattgct tcttgttgca ggattgctga ccactgcctg tgggatgttt
     2341 tggcgttttc ctcagttttt ccagtcactt ccggcatcgc tgggcggaga tgagagtgtg
     2401 tccaatggag agcgcatata cttcactgcg accaatgtag atggagattg ggtcagatac
     2461 tcagggggtc caaggtttgg cggcatgatg atgggatcgt atttaacctg cgctgcctgc
     2521 catgggccag aaggacgcgg gggcattcat acgatgcaca tgcaggtgat ggacgcaccc
     2581 gatatccgtt atgtggcgct gagcgaagaa gcgggcgagc acaaagatga gcaacaagat
     2641 gatcatagtg atgcgcacgg agaatataca ttggatgatt tccgcttggc agtggtagag
     2701 ggggaacatc cggatggcca gccgctcagc cgggatatgc cgcgatggag aatgagtgaa
     2761 aaggacctag ccgatttatt tgaattctta aaatcgctac cttaaaagaa cttacggggt
     2821 ttgatggcgg gcacgcccag ctcatatttc ttaagtctat aatgagaaat cagccacacg
     2881 taattgtata attaaggtaa atattatata ataatt
//
LOCUS       DBNHOH010000775         1434 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_753733, whole genome shotgun sequence.
ACCESSION   DBNHOH010000775 DBNHOH010000000
VERSION     DBNHOH010000775.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_753733"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="AC16V7_13880"
     CDS             <1..482
                     /locus_tag="AC16V7_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6258258.1"
                     /translation="GDMRTVRLSRVYDAVFIHDAISYLTSLSDLEQAIRTAWIHCRTG
                     GVALFAPDFTRETFQAGTSHGGYDAAMRGMRYLEWRWDPDPSDATYVVDFAYLLRDES
                     GDVRVEADRHHMGLFSRLEWLETIHQAGFEASAIPFEHSEIEAGSSEIFVGVKPTER"
     gene            750..1217
                     /locus_tag="AC16V7_13885"
     CDS             750..1217
                     /locus_tag="AC16V7_13885"
                     /inference="COORDINATES: protein motif:HMM:NF024212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HIR6258259.1"
                     /translation="MSQPFEEMHFIEDIGLFFEQMGMPRMAGRILGVLLISDPPAQSI
                     TDLSEKLKASKSSISIMARLLVENGLIERVASRVPRRDYYRFKPGGWILYMEQWLGLM
                     AALHQITERGLSLIDEKPPELKERLQEAHDLFSFIEEEFPALLEALQKKRASE"
     gene            1252..>1434
                     /locus_tag="AC16V7_13890"
     CDS             1252..>1434
                     /locus_tag="AC16V7_13890"
                     /inference="COORDINATES: protein motif:HMM:NF012893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HIR6258260.1"
                     /translation="MTLLTNTQATRNAQSEEIHQHEVFTGLTSAEVLKQRDIYGKNVL
                     PAEEKTSAWTIFINQLK"
BASE COUNT          379 a          349 c          360 g          346 t
ORIGIN      
        1 tgggtgacat gcggacagta aggctcagcc gcgtctacga tgcggtcttc atccatgacg
       61 cgatcagcta tctaacctcg ctcagtgacc tcgaacaggc tatccggacg gcgtggatcc
      121 actgccggac tggaggtgtg gcgctcttcg cacccgattt cactcgagag acgtttcaag
      181 caggcacaag ccacggcggc tatgacgccg ccatgagggg gatgcgatat cttgagtgga
      241 ggtgggatcc agacccgtct gatgcaacct atgtggtgga tttcgcctac ctattacggg
      301 atgaatcagg cgatgtgcgg gttgaggcgg accgccacca tatgggactt tttagccgat
      361 tggagtggct ggagacgatc catcaggctg gttttgaggc aagcgcgata ccctttgaac
      421 acagtgaaat cgaagccggt agcagcgaga tattcgtggg ggtcaagcca actgaacgat
      481 aacccagcta tgatgcactc gtagcacaat cgagatcgcc gcccttcagc aaccactcgc
      541 tagggaatat gatagaacga gcaattagat tagtcccccg ataaatattt ttccatctaa
      601 tatctcgaag caataaattc atatccggct gctcatttgt agtaatggac aaccgtttaa
      661 ttgccgtttt gtttgtaacg tttagtattt acaaaacgtt tctaaagttt tatacttaat
      721 tacataatct cactctgcga gtattgacta tgagccaacc ttttgaagaa atgcatttta
      781 tcgaggatat tggtctgttc tttgaacaga tgggcatgcc gcgcatggct gggcgcatct
      841 taggcgtgtt gctgatctca gatccaccag cacagtccat cacagacctg tccgaaaaat
      901 taaaagccag caagagctcc atcagcatta tggcgcgctt gctggtggaa aacggcctga
      961 tcgagcgcgt cgcttcgcgt gtaccgcgcc gcgattacta ccgctttaag cccggtggct
     1021 ggattttgta tatggaacag tggctggggt taatggcggc cttgcatcag atcaccgaac
     1081 gaggcttatc tttgatagat gaaaaacctc cagaattgaa ggaacgttta caagaagccc
     1141 acgacttgtt ttctttcatc gaggaagaat tccctgcgct gctcgaagca ttacaaaaaa
     1201 agcgcgcatc agaataatca tgctttcatc acaataatcg aggggatcat catgactttg
     1261 ctcaccaata cccaagcgac tagaaacgcg caatctgaag aaatccacca gcacgaagta
     1321 tttaccggcc tgaccagcgc cgaggtgctt aaacagaggg acatctatgg caaaaacgtt
     1381 ctgcccgctg aggagaaaac ctcagcgtgg acgattttta taaaccagct caag
//
LOCUS       DBNHOH010000776         3910 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_48138, whole genome shotgun sequence.
ACCESSION   DBNHOH010000776 DBNHOH010000000
VERSION     DBNHOH010000776.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_48138"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..1131
                     /locus_tag="AC16V7_13895"
     CDS             208..1131
                     /locus_tag="AC16V7_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6258261.1"
                     /translation="MTASQLENPIEVSSPKGARLALNALFLGALAIAFAPIFVRMSQV
                     GPSSTAFWRLVLSLPVLFAWMQLEGRGTQAPRRPSTRSDYIQLIVAGLFFTGDLGVWH
                     WSIRFTTVANATLLANFAPIFVALGSWLLFRQGVTRAFVFGMVMALAGTVFLVGDSFR
                     LSLQHLWGDILGLITAVFYAGYILSVKELRREFSTSTILTWSGLVSSIALLPIAQLSG
                     ESLLPASAAGWMVLVALALISHFGGQGLITFALAKLPAAFTSVVLLVQPIAATIFAWL
                     ILGETLGVWQGLGGALALAGIYVARRASQIG"
     gene            1195..2088
                     /locus_tag="AC16V7_13900"
     CDS             1195..2088
                     /locus_tag="AC16V7_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6258262.1"
                     /translation="MKNKSSIALSPWLVLFAAMLWGTTGTAQALAPDGAQPAIVGTAR
                     LAVGGVALLALAAARRALNKGGRWPLMTTLIAAACMAAYQLFFFAGVARTGVAVGTVV
                     GIGSSPILAGIIGFLVRGERPGRKWATATALAILGCSLLITAGQSIQVDPLGLLLATG
                     AGAAYACFTVASKYLLEDRPPETVMAVVFCIGALFLLPFFFTADLAWLMKPRGLAVVL
                     HLGLVTVAIAYTLFARGLSKVPVATAATLTLAEPLTAGILGVFFLGERLTPLATFGIF
                     LIFAGLAIISVGRKAKGATPA"
     gene            2232..2678
                     /locus_tag="AC16V7_13905"
     CDS             2232..2678
                     /locus_tag="AC16V7_13905"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR6258263.1"
                     /translation="MLSSSLMDMIREKYADKTGELLIPPPVFDEMAGEFVALDLDKRT
                     LSTRFPVQTKYFNPYRAVQGGIIAAAVDNTLGPLSMLVAPPSVTRRLEMKYSRQVGVE
                     SEYISVVGRLVEQKERNLIFSAEVRDSKGNLMARARSTHWIVETPG"
     gene            2834..3397
                     /locus_tag="AC16V7_13910"
     CDS             2834..3397
                     /locus_tag="AC16V7_13910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6258264.1"
                     /translation="MTTMLEALNPEQIARAQRGDSATISDLYDRYRLSVYRYLYYKVG
                     DLHAAEDLTSEVFLRMIRSLNSYRPQNSSFEAWLFQIARNLAIDHYRQKNTRGHLSLE
                     EHMIHDTDDPDQKYDRSLTSDLLLKALSRLSEVQRDVIVLRFVNGLPITQVAQTLHKS
                     EDAVKGLQRRALGSLREILTEWEVTYA"
     gene            3390..>3910
                     /locus_tag="AC16V7_13915"
     CDS             3390..>3910
                     /locus_tag="AC16V7_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258265.1"
                     /translation="MPNYDDILEKKLEALEKGQPLDKALADLPNEAEDLGALLQLASS
                     IRAMTHPQPASVLARNRLENAAAREKTRPITHPRILSPQPRSKGPLESILSSLPRSLV
                     PQLRLGMAVGFAAAMLVLLFSLVAFFSLAGPGRAKAATLTEVSGVVRVASARGGDDWV
                     PVEAGDKVRAGQR"
BASE COUNT          801 a         1109 c         1071 g          929 t
ORIGIN      
        1 caccaaaaca cccgaaacac tctttttacg ggattgcgca tgcccctcag gtcatatgca
       61 attgccctgc cccctctcac gacctgcctc cgacgtttat actcactgat ataattggag
      121 cttccgcagt aataaggaga acgcccactg aactatgtat agtcgatagt ctcgcgagca
      181 aaattaagtc tattacgatt attccatatg actgcttccc aacttgaaaa ccccattgag
      241 gtatccagcc caaaaggcgc tcgcctggcg ctgaatgccc tattcttggg cgcgctggct
      301 atcgccttcg cccctatctt tgtccgcatg agccaggtgg gtcccagcag cacagccttt
      361 tggcggctgg tactctccct acctgtgctc ttcgcctgga tgcaacttga gggccggggg
      421 actcaagcgc cacgaagacc ttcgacccgc tccgactata tccagctgat tgtcgctggt
      481 ctgtttttta ccggcgatct gggagtgtgg cattggtcaa tccgtttcac aaccgtggcc
      541 aatgccaccc tgctggcgaa cttcgcgccg atatttgttg ctctgggaag ctggctgctt
      601 ttccggcaag gagtcacccg ggcttttgtc ttcggcatgg tgatggctct ggcaggcact
      661 gtcttcctag taggggatag cttcaggctc agcctgcagc acttgtgggg agatatcctg
      721 gggcttatca ctgccgtgtt ctatgccggt tatatcctct cggtcaagga gctgcgccga
      781 gagttctcca cctctactat cttaacctgg tctggcttgg tgagcagcat tgccttgctg
      841 cccatcgcac agctcagcgg cgagagcctg ttgcctgcca gcgcagcggg ctggatggtt
      901 ctggtcgctc tggcgctgat ttcgcatttt ggagggcaag gtctgatcac ctttgcccta
      961 gccaaactgc ctgccgcgtt cacatcagtc gtgctgctcg tgcagcccat cgcggcgacg
     1021 attttcgctt ggttgatcct gggtgaaacc ctaggagtgt ggcaggggct tggcggcgcg
     1081 ctggcgctgg cgggtattta cgtcgcccgg cgagccagtc agataggcta gccatttctc
     1141 gccgtcgcaa gcgcaccatt ccccacaata gcgagatttt tctcagacgc accgatgaaa
     1201 aacaagtcta gcatagctct tagcccctgg ttggtcctgt ttgcagccat gttgtggggc
     1261 accaccggga cagcgcaggc cctcgccccc gatggagccc agccagctat tgttggcacg
     1321 gcccgcttag ccgtgggggg cgtcgcacta ttggcacttg ctgccgcgcg ccgcgcatta
     1381 aacaagggcg ggcgctggcc attgatgacc acgttaattg cggccgcctg tatggcagcc
     1441 taccaactct ttttcttcgc cggtgtggcc agaacaggcg tggcggtggg caccgtggtt
     1501 gggatcggca gctctcctat cctggcaggg attattggct tcctcgtgcg cggcgaacga
     1561 cccgggcgca agtgggctac cgccactgcc ctggcaattt tgggttgcag cctgctcatc
     1621 acggctggtc agagcatcca ggtggacccg ctcggcttgc tactcgctac aggtgcaggt
     1681 gcagcttatg cttgcttcac cgtcgccagc aagtacctgc tggaagacag accgcctgaa
     1741 acagtgatgg cggttgtctt ttgcattggc gcgctcttcc tgcttccatt ctttttcact
     1801 gccgatcttg cctggctgat gaaaccgcgc ggcctggcgg tggtcttgca tttggggttg
     1861 gtcaccgtgg caatcgccta cactttgttc gctcgcgggc tgagcaaggt gcctgtggca
     1921 accgccgcca ccttgaccct ggcagagccg ctcactgctg gcatactagg agtgttcttt
     1981 ctgggagaac ggcttactcc tctggcaacc tttggcatct ttttgatttt cgctggcctg
     2041 gcaataatct cggttggacg taaggcgaag ggtgccactc cggcctgaag ccttttttaa
     2101 ttgcaaaaat ttaacagtaa ttgtgctata ctgaaatata taataccttc ggtatagaca
     2161 cggtcaagag cggggctttt cattgcacat cccttgactt caagcacatt cgcaatctga
     2221 ggtgacggcc catgctatct tcttccttga tggatatgat tcgggaaaag tatgctgaca
     2281 aaacgggtga actcctaatt ccgccccctg tcttcgatga gatggcgggc gagttcgtgg
     2341 cgcttgatct ggacaagcgc acgctttcga cgcgcttccc ggttcaaacg aaatacttca
     2401 atccttatcg agctgtgcaa ggcggcataa ttgctgccgc ggtggataat acgctggggc
     2461 ctctcagcat gctcgttgct cccccaagcg taaccaggcg attagagatg aagtacagcc
     2521 gtcaggttgg tgtggagagc gaatacattt cagtggttgg caggctggtc gaacaaaaag
     2581 aacgcaacct gatattcagc gcagaggtgc gagactcgaa gggaaatctg atggccaggg
     2641 cgcgctccac ccactggatt gtggaaacac ccggctagtt tggcacagct aatggatagt
     2701 aatcatggtt tgacgtggtt atttctccca gaaattacta ttttatttat cgtttaatta
     2761 aaatctagtt aaacttaccc tttttatgac tcgttttctc acttaatccg ttaagcatgt
     2821 tagacggaga aagatgacta caatgcttga agctctcaac ccagaacaga tcgcccgcgc
     2881 acaacgaggc gactccgcca ctatcagcga cctgtacgat cgctaccgcc tgagtgtata
     2941 ccgttatctg tattacaaag taggcgatct gcacgctgct gaagacctga cctcagaagt
     3001 gtttttacgc atgatccgct cgctgaatag ctaccgacct caaaattcat ctttcgaggc
     3061 ctggctgttc cagatagccc gcaacctggc aatcgatcat tatcgccaga aaaacaccag
     3121 aggccatttg agtctagagg aacatatgat acatgataca gatgatcccg accaaaaata
     3181 tgaccgcagc ctgaccagcg acctgctcct gaaggcgctg tccagattga gcgaagtaca
     3241 gcgcgatgtg atcgtgctgc gctttgtcaa cgggctgccc atcacccagg tcgcccagac
     3301 gctgcataag agcgaagatg ctgtaaaagg cttgcaacgc cgcgccctgg gatccttgcg
     3361 tgagattctg accgaatggg aggtcaccta tgcctaatta tgatgatatc ctagaaaaga
     3421 aacttgaggc cctggagaag ggccagccat tggacaaagc cttggctgat ttgcctaacg
     3481 aggcagaaga cttaggggcc ctgctccagt tggcttcatc catccgggcg atgacccatc
     3541 cccagccagc cagtgtcctg gctcgcaacc gactggagaa cgctgcggca agggagaaga
     3601 cccgcccaat aacccacccg cgaatcctct cgccgcaacc ccgctccaaa ggaccgctgg
     3661 agagcatcct gtcttccctg ccgcggagcc ttgtgcccca attacgcctg ggtatggcgg
     3721 taggattcgc cgcggcgatg ctggtgctgc tgttcagcct ggtagctttc ttcagcctgg
     3781 caggaccagg ccgagccaag gccgccaccc tgacagaggt gagcggtgtg gtgcgcgtgg
     3841 cctcagccag gggaggcgac gactgggtgc ctgtcgaggc aggcgataag gtgcgagccg
     3901 ggcagcgcat
//
LOCUS       DBNHOH010000777         3054 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1074638, whole genome shotgun sequence.
ACCESSION   DBNHOH010000777 DBNHOH010000000
VERSION     DBNHOH010000777.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1074638"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="AC16V7_13920"
     CDS             complement(<1..449)
                     /locus_tag="AC16V7_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HIR6258266.1"
                     /translation="MFTSVVEAIGNTPLVELSRISRDLNGRILAKLEYLNPGFSKKDR
                     IARQMIEEAEGSGALKPGQTVVELTSGNTGTGLAFVCTVKGYPFVAVMSRGNSMERAR
                     MMSALGAEVVLVDQLPDSKPGYVSGGDLELVEAAAQRIVRERGAFRA"
     gene            603..1592
                     /gene="corA"
                     /locus_tag="AC16V7_13925"
     CDS             603..1592
                     /gene="corA"
                     /locus_tag="AC16V7_13925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HIR6258267.1"
                     /translation="MIRSLYITRDGLVKTDLPLGAFAEALRDQDGLLWVDFEGTPPET
                     DEPILRDVFGFHPLAVDDALQESHVPKVDDWGEYLYIVLHAVIFDRTDGGHVDTLELD
                     VFLGRNFMVTHHDQNIAAIERVWSASQRDERLWKNGVDHILYRLTDEVVASYMPVVEE
                     IDEAVDRAEEEVFERATPETLSKIFALKRAVLHLRRIIGPQREVLNKLARDDYPAIDT
                     QARVYFRDVYDHLVRLHDITESIRDLVSGTLDTYLSVINNRMNDIMKTLTIITTMFMP
                     ISFVVGFFGMNFFQPVAASLTPWADTPVFTITLAITILTPLLMYWWIRRRGWM"
     gene            1646..2125
                     /locus_tag="AC16V7_13930"
     CDS             1646..2125
                     /locus_tag="AC16V7_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HIR6258268.1"
                     /translation="MGKQLSGSAQKVQEALKNLGFTLNVVELPNSTRTAVEAAQAVGC
                     QVGQIVKSLVFKAKRSQRPILVVASGSNRVNEKAIEALIGEPLGKADADFVRQRTGFV
                     IGGVPPVGHVEPLQTFIDQDLLGYAEIWAAAGNPNAVFRLTPEDLIRMTSGVVVKIT"
     gene            complement(2172..2894)
                     /locus_tag="AC16V7_13935"
     CDS             complement(2172..2894)
                     /locus_tag="AC16V7_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258269.1"
                     /translation="MKTRPLGILTLIVALSSILWIIFLIIWEAVTPPVNSLNEQVAAI
                     QAHFGLFILNYANAGVITLATVALYTGLCDFCRDDDSLWSWIALVFVPIYGLANLVVY
                     LSQIFVVPGLIDLYQNPETVGVAAILLSLTIHTWSGSAMEFVNALAYAILGIPSIILG
                     ILMYRKARGLRIGSGLLAISGMLSLVALLGVAIRNAWLASTTLLSGAVSLVALILIGL
                     YFLRQSALWSSDHDERRVVGEQ"
BASE COUNT          739 a          883 c          794 g          638 t
ORIGIN      
        1 tcggctcgaa aggcgcccct ctcccgcacg atgcgttggg ctgctgcctc cacaagctcc
       61 aggtcgccac ctgagacgta gccaggcttg gagtctggca actggtccac cagcaccact
      121 tcagcaccca gagcagacat catgcgcgcc cgctccatcg aattgccgcg tgacatgact
      181 gcaacaaagg ggtagccctt caccgtgcag acaaacgcca agcccgtgcc ggtattgccg
      241 ctggtcagct cgaccacggt ctggcctggt tttagcgccc cgcttccctc ggcctcctca
      301 atcatctggc gggcaatgcg gtctttcttg gaaaatccag ggttcaagta ttccagcttg
      361 gccaggatgc gaccgttaag atccctggaa attcgggaaa gctctaccag aggcgtgttt
      421 cctatggctt caacaacgga ggtgaacatg gtagtcctcc ttcttttcaa cgattataac
      481 gacaaactcc caccccagag gagcgtgcga ttttgcattc catgattgac acaacgtata
      541 tcgctgcgat agactctccc tatatatcat catccaaccc gtgtctggcg aggagcatgc
      601 ccatgatccg ttccctatac ataacaagag atggcctggt gaaaaccgat ctccctctag
      661 gagctttcgc tgaagcactg cgcgatcagg atggtctcct gtgggttgat tttgagggta
      721 caccgccaga aaccgacgag cccattctgc gggatgtctt tggctttcac cccctggcag
      781 tggatgacgc cctgcaagaa tcacacgtcc ccaaggtgga cgattggggc gagtatcttt
      841 acatcgtctt gcacgccgtg atcttcgatc gcacagatgg cggtcatgtg gacacgctgg
      901 agctggatgt gttcctgggg cgcaacttca tggttaccca ccatgaccag aacatcgcgg
      961 ccattgaaag agtctggtca gccagccagc gtgacgagcg cctgtggaag aacggtgtcg
     1021 atcacatcct gtaccgcctg accgacgagg tggtggcaag ctacatgccc gtggtggaag
     1081 agatcgatga ggccgtcgac cgcgccgagg aggaggtatt cgagcgcgcc acgccagaga
     1141 cgctttccaa aatatttgcc ctgaaaaggg ccgtgctgca cctgcgccgc atcatcggcc
     1201 cgcagcgaga ggtgctcaac aagctggcgc gtgacgatta ccctgccatc gacacccagg
     1261 cgcgcgtcta cttccgcgat gtgtacgatc acctggtacg cctgcacgat atcaccgaga
     1321 gcatccgcga tctggtcagc ggcacgctgg acacctacct gtcggtgatc aacaatcgca
     1381 tgaacgacat catgaaaacc ctgaccatca ttaccaccat gttcatgccc atctcatttg
     1441 tcgttggttt cttcgggatg aactttttcc agccagtggc ggcctcgctg acgccttggg
     1501 cagacacgcc cgtatttacc atcaccttgg cgattaccat cttgaccccc ctcctcatgt
     1561 actggtggat tcgtaggcgg ggctggatgt gattacaacg cgcaaatact agacgcgcga
     1621 gaaatattat ctagagagga tgccaatggg aaaacaactg agtggcagcg cacaaaaagt
     1681 tcaggaggcc ttaaaaaatc tgggcttcac cttaaacgtg gttgagctgc ctaattcgac
     1741 ccgcaccgcc gtggaggcag cccaggctgt tggctgccag gtgggccaga tcgtcaaatc
     1801 actggtcttc aaagccaagc gcagccagcg ccccatcctg gtggtcgcta gcggctccaa
     1861 tcgcgttaac gagaaggcta tcgaagcgtt gatcggcgag ccgctgggca aggcagacgc
     1921 cgactttgtg cgccagcgca ccggatttgt catcggcggc gtccctcccg tcggtcatgt
     1981 cgaaccccta caaacattca tcgaccagga cctgctgggc tatgcagaga tctgggcagc
     2041 ggcgggcaat cctaacgctg tcttccgcct gacacccgaa gacctgatac gcatgacaag
     2101 cggggtggtc gttaaaatca cctaggcgtt ctttttccga aatgtgtctt tttacaattg
     2161 acgcgacggt atcactgctc ccccactacc cgtcgctcat catggtcaga agaccacagc
     2221 gcggattgcc tgaggaagta cagcccaata aggatcaaag ccactaaact gacagcaccg
     2281 ctgagcagcg tggttgacgc cagccaagcg tttctgatgg cgacgccgag cagagccacc
     2341 aacgacagca ttccgctgat cgccagcagc ccgctgccaa tgcgcagacc tcgagccttg
     2401 cggtacatca agatacctaa gatgatcgac gggatgccca ggatagcata cgccagggcg
     2461 ttgacaaact ccatagccga ccctgaccag gtatgtatcg tcagactcaa taaaatcgct
     2521 gccaccccaa ccgtctccgg attttgatac aggtcgatca atcccggcac aacgaaaatc
     2581 tgtgatagat acaccaccag gttcgccaga ccatagatcg ggacgaagac cagcgcgatc
     2641 caagaccaaa gcgaatcatc atcccggcag aagtcgcata accctgtata caacgccact
     2701 gtggcaaggg tgattacgcc agcattggcg taattcagga taaacaggcc aaaatgggct
     2761 tggatggcag ccacttgctc gttcaacgaa ttcacgggag gggtaacagc ttcccagata
     2821 atcaaaaaaa ttatccataa gatcgaggag agggcgacaa ttagcgtaag aatgccaagg
     2881 gggcgtgttt tcatactttc aagcctcttg ctggcaagaa ttataaacaa aaatgcttgg
     2941 aaaaccaatt tgtggaccca ggagggatca ctaaacactg aatttgtggt ataactttga
     3001 taaaattagt ctaaattaat ctgctatggc tctcgaccgc tttggacgct ccat
//
LOCUS       DBNHOH010000778         1832 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_48290, whole genome shotgun sequence.
ACCESSION   DBNHOH010000778 DBNHOH010000000
VERSION     DBNHOH010000778.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_48290"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="AC16V7_13940"
     CDS             complement(<1..700)
                     /locus_tag="AC16V7_13940"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HIR6258270.1"
                     /translation="MSYDPIFIAGPDRSGTTLIYALLASHPNISMVRRTNMWRYFHKR
                     YGELSQPENFERCLNDMVRFNRMRQLQPDPERIRQEFWQGEPTYGRLFALFHEHVAER
                     RGKKRWGDKSLHTERHADRVLAEYPNAKIIHISRDPRDRYASVRKRHGKDMPRLGAAT
                     GRWLFSMSAARRNLKRYPNNYMIIKFEELASQPEEVLRKVCAFIGEEYTPAMLTMKGA
                     ADHRDKGGNSSFGEI"
     gene            complement(704..>1832)
                     /locus_tag="AC16V7_13945"
     CDS             complement(704..>1832)
                     /locus_tag="AC16V7_13945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6258271.1"
                     /translation="SGCAPVVCTFKDGPLREPIESLGIPVEVLPERRANILALPSFLM
                     EMLRIRRDLANLIQKYEIDVIQTHLLRSLDFLVLSLRFGRDLLVFWTFQNAVFDLRED
                     HLTRHKWLLKPKRFAHRLLYRLGSRWVTGFIAVSQDTKKAILEYLGHLPDDKITVIFN
                     SVDVKRYHREVDRGSIRRQLGLADSDHVMSVVATFKKQKGHCYLIEATSLIIQEMPDI
                     HILFIGDGELREELMTQTKSLGLERNIHFLGTRKDVPELLAASDYFILPSLWEGLSMA
                     LVEAMASQIPVIATDVSGTKQVIIPGETGILVPPGNAQQLANAMLQIVSDPEKAKAMA
                     IAAKQRVEKYFSAQKQAKDHIALFERELNKTNWQPALSIRR"
BASE COUNT          439 a          453 c          431 g          509 t
ORIGIN      
        1 taatctcgcc aaatgagctg ttcccacctt tatcgcgatg atctgctgcg cctttcattg
       61 tcagcatagc aggtgtgtac tcttcaccga taaatgcaca caccttgcgc agcacttctt
      121 ctggttggct tgccagttct tcaaatttga taatcatgta attgtttgga tatcgcttca
      181 ggttgcgcct cgccgcgctc atagagaaca accatctacc ggttgcagca ccgagcctgg
      241 gcatgtcttt accatgtcgc tttcgcacgg atgcgtacct gtcgcgagga tcccgactaa
      301 tgtgaatgat tttggcattg gggtattctg ctaatacccg atctgcatgg cgttctgtat
      361 gcaaggattt atcaccccag cgctttttac ctcttcgttc tgcaacatgt tcgtgaaaca
      421 aggcaaacaa gcgaccataa gttggttcgc cttgccagaa ctcttgtcga atgcgctcgg
      481 gatcgggctg taattgtcgc atgcggttga aacgcaccat gtcgttaagg caacgctcaa
      541 aattttccgg ttggcttagt tctccatatc gcttgtgaaa ataccgccac atatttgtcc
      601 gtcggaccat ggaaatattt ggatgtgagg cgagcagggc gtaaatcaac gttgtgccac
      661 tgcgatctgg cccagcaata aatattggat cgtaactcat tcactatctc cttatcgata
      721 aggcgggttg ccagttcgtc ttattcagct ctctttcgaa cagagcgata tggtccttag
      781 cctgtttttg agcactaaaa tatttttcta cccgttgctt ggctgctatt gccattgcct
      841 tggctttttc tgggtcggac acgatctgta gcatcgcgtt ggctagttgc tgtgcattac
      901 caggtgggac taagataccg gtttcgccgg gaatgatcac ctgcttggtc cctgaaacat
      961 ctgtcgcgat cactggtatc tggctggcca tggcttccac cagagccatc gaaagcccct
     1021 cccaaaggga gggcagaatg aagtaatcgc tggctgctaa taactccggc acatccttgc
     1081 gagtacccaa gaaatggata tttcgctcaa gccctaagga tttcgtttgt gtcatcaact
     1141 cttctcgtaa ttcgccatcg ccaatgaaca gaatgtgtat atcgggcatt tcctggatga
     1201 tcagggatgt ggcttcaatc aaatagcagt gccccttttg cttcttgaat gtggcaacca
     1261 ctgacatcac gtggtcagaa tcagctaatc ctaattggcg gcgaatggag cctctatcca
     1321 cttctctatg gtagcgtttg acatccacgc tgttaaaaat gacggtgatc ttatcatcag
     1381 ggagatgacc gagatattct agaatcgctt ttttggtatc ctgcgaaaca gcgatgaaac
     1441 cggtcaccca gcgggatccc aagcggtaca acaggcggtg ggcgaaacgc ttcggtttaa
     1501 gtaaccattt atgccgcgtt aagtgatctt cgcgcaggtc aaatacggcg ttctggaaag
     1561 tccagaaaac caggagatct cttccaaatc gcaggctcaa aacaagaaaa tccaggctcc
     1621 ttaataggtg cgtttggatg acatcaattt cgtatttttg aattaaattt gctaagtctc
     1681 tccgtatgcg cagcatttcc atgagaaatg aaggaagagc caaaatattt gcgcgccgtt
     1741 cgggtagcac ttctacggga atacccaggc tttctattgg ttcgcgcaac ggaccatcct
     1801 tgaaagtaca cacgactggg gcacaaccag ac
//
LOCUS       DBNHOH010000779         2235 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1394526, whole genome shotgun sequence.
ACCESSION   DBNHOH010000779 DBNHOH010000000
VERSION     DBNHOH010000779.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1394526"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1603)
                     /locus_tag="AC16V7_13950"
     CDS             complement(<1..1603)
                     /locus_tag="AC16V7_13950"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_function: GO:0004574 - oligo-1,6-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009311 - oligosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HIR6258272.1"
                     /translation="MTELKWWQRAVFYQIYPRSFADGNGDGIGDFPGMFQKLDYLQDL
                     GIDAVWLSPHYPSPQFDCGYDITDYKGIAPEYGTLSEFNRFIAGAHQRGIRVILDLVL
                     NHTSHEHPWFIESRSSRHNPKRNWYVWKDGKVDDNGILQPPNNWNSTFDGIAWEFDPP
                     TNQYYYHYFLKQQPDLNWHNPEVKREMWDSVRFWLDLGVDGFRLDAINTIYERPEMPK
                     HPLDMSLASLRRILRDSRTPEEHAQARRLQKLMFQYQNHQPGLHELMQELRSVIDEYD
                     DCVLVAEDDDIAYMGNGDNELHLVFNFPLMRTSRLTPAWVRANQKERLTTLASISPSA
                     WPCNTLGNHDSPRVYSHFGDGKNDQQLARISLALMLTLRGTPFLYYGEEIGMTDNYLE
                     HIDDFRDLLGTWQYQAEIDEFGTPPDLALQYAAKLTRDKNRTPMQWENSPNSGFSPAN
                     VRTWLPVNTNYDSGVNVADQAADPASLLKFYQRILSLRKNNPALIGGDYLPLHEHSDD
                     YLAFLRRAEQQTCLVVLNMVAKHLRI"
     gene            complement(1868..>2235)
                     /locus_tag="AC16V7_13955"
     CDS             complement(1868..>2235)
                     /locus_tag="AC16V7_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258273.1"
                     /translation="RLLKIVYKGDQPVGFLLAYPDISAAVQRTRGRIFPLGWIDLLLE
                     LRRTKWININGAGMLEGYRGLGGTALLFSEMHKSVTEEGYQHADLVQIGTENERMLRE
                     LRGLGIDFYKTHRMYSRTM"
BASE COUNT          580 a          509 c          565 g          581 t
ORIGIN      
        1 ggatacgcag atgtttagca accatattca acacgaccag gcaggtttgt tgctctgccc
       61 tgcggaggaa agctaaatag tcatctgagt gctcatgtag gggaagatag tcgcccccaa
      121 tcaatgctgg gttatttttg cgtaagctca gaatacgttg ataaaatttc aagagggaag
      181 ctggatcagc agcctgatca gccacattca cgccagagtc gtaattggtg ttcacgggca
      241 accaggttcg aacatttgcg ggactgaagc ctgagttagg tgagttttcc cactgcatgg
      301 gagtgcggtt cttatcgcga gtgagtttag cggcgtactg caaggcgaga tcgggtggtg
      361 tgccaaattc atcgatttct gcctgatatt gccaggtgcc aagaagatca cggaagtcat
      421 cgatatgctc taaataattg tcggtcatgc ctatctcttc gccataatag agaaatggcg
      481 tcccacggag tgtgagcatg agtgctaagg atatacgggc aagttgttgg tcatttttgc
      541 catcgccgaa gtgactatat acgcgcgggc tatcgtgatt gcccagagtg ttgcacggcc
      601 aagccgaggg agatatcgag gctaatgtag tcaggcgttc tttctgattg gcgcgcaccc
      661 aggcgggggt caatcgactt gtgcgcatca atgggaaatt aaagaccaaa tgaagctcat
      721 tgtcgccatt tcccatgtaa gctatatcgt catcctcggc aaccaggacg caatcgtcat
      781 attcatcaat gaccgaacgc agctcttgca ttagctcatg caagcctggt tggtgatttt
      841 gatattggaa catgagcttt tgtaaacgac gtgcttgtgc atgctcttcg ggtgtgcggg
      901 aatctctcag gattctgcgc aaacttgcaa ggctcatgtc taagggatgt tttggcatct
      961 ccgggcgttc atatatcgta ttgattgcat ctaagcggaa tccatccacc cccagatcaa
     1021 gccagaaacg aacggaatcc cacatttcac gcttgacttc ggggttgtgc caattgagat
     1081 cgggttgctg ctttaaaaag tagtgataat aatattgatt tgtgggaggg tcgaattccc
     1141 aagcaatccc atcgaaagtc gaattccaat tatttggggg ttggagtatg ccgttatcgt
     1201 cgactttacc atctttccaa acataccaat tgcgctttgg attgtgccgg ctggatctgg
     1261 attcaataaa ccagggatgt tcatgagagg tgtggttcaa aactagatcg aggatgacac
     1321 gaatcccgcg ctggtgtgca ccggcgataa agcgattgaa ctctgagagt gtgccatact
     1381 cgggtgctat gcccttataa tcggtgatgt cgtaaccgca atcaaactgt ggggatggat
     1441 aatgtgggga gagccaaacg gcatcgatac ctaggtcttg aagataatca agcttttgaa
     1501 acatgccggg gaaatcacca attccatcac cgttaccatc ggcgaagcta cgtgggtaga
     1561 tctggtagaa gacggcacgc tgccaccact ttagttctgt catcgaatga gtttcctgga
     1621 tattctggaa aaattattta atgattgaac ctgaatatgc ggttaaattc ttcatcaaaa
     1681 ttgcctcaca gcaacaagca gtgacacata aagcacagtt gctacaacgc gaatactcta
     1741 tatatggcgc ctcgcccgga tcaatctgga caatagcgcg agtccggcaa gcgtggcctg
     1801 ctaaacaagg caggcaactt tggcaaatac cccagtcaat ttcgggtcgc aacatgaata
     1861 caatgaccta catggtacga gaatacatgc ggtgggtctt gtaaaaatcg atgcccagac
     1921 ctcgtagctc tcgtagcatc ctttcgtttt ctgtgccgat ttgaaccagg tcggcatgct
     1981 gatacccctc ttcggtgacg cttttatgca tctcactgaa gagcaaagca gtgccgccta
     2041 gtccccgata cccttcaagc attcctgctc cgttaatgtt aatccatttg gtgcgtcgta
     2101 gctcgaggag aagatcaatc caacctaaag ggaagattcg cccccttgta cgctgaacag
     2161 ctgcggatat atctggatag gcgagcagaa aaccaactgg ttgatctccc ttgtagacta
     2221 tctttaataa tcgag
//
LOCUS       DBNHOH010000780         1719 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_882104, whole genome shotgun sequence.
ACCESSION   DBNHOH010000780 DBNHOH010000000
VERSION     DBNHOH010000780.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_882104"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(251..1402)
                     /locus_tag="AC16V7_13960"
     CDS             complement(251..1402)
                     /locus_tag="AC16V7_13960"
                     /inference="COORDINATES: protein motif:HMM:NF039823.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HIR6258274.1"
                     /translation="MAEKREGIEQILHTCLELIRSGEESVDSALARYPDHADELSSLL
                     ETATWLQERRDALAPRPEFIAASRQRLLARIEQEKAASTPKSLRTVGASVWNTLLSLF
                     AQKRFAYQFVVATILLVFLISTTSGVAAAAQHSIPGDTLYGVKMSLEQIQLALSFSEA
                     RRAQYYVTFAERRLVEVQSLVIEGRYEYLHNTVDQFYSQANEASRLLRSLGTTNTLLA
                     RELANKLRNVVEGQISLMPVLSQAAPPDSRSEIERLMQMAELVKSEAQNIEALMGGTP
                     LPTITPTLITITPVPPTPVPTKTPVPTTAPVVTVAPLSTPLSAKTEAAPTLTVRPSRT
                     PVPTLQNSGKKPTPTPTKASPAKPTRKPTKEKKEPPGLENRPPKPTKKS"
     gene            complement(1377..>1719)
                     /locus_tag="AC16V7_13965"
     CDS             complement(1377..>1719)
                     /locus_tag="AC16V7_13965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6258275.1"
                     /translation="NALIDHYRRERRTAGQLSSEDVMIADHKPGPGEVVSQQMEHKEL
                     REALDDLKDDYRNVLVMRFLSDMSPEETAHAMGRSAGAVRVLQHRALAALRTKFEKSK
                     GKRDGRKARRD"
BASE COUNT          396 a          433 c          437 g          453 t
ORIGIN      
        1 gtccacggtc cacttgtcga gatttgcgca aaatacccag catagaaacc attttcctca
       61 aattacaatg cattatgcag aaaatataat taccacatgt aaatatgcgt ttaataagta
      121 caaaaaaagc cacttacgcg acatgtcacg ctgcacaccc atatattaca tcatgagcgc
      181 attaattacg cttacagttc tgttatcagg cagttaaaat acaattcgac tgaaatcgtc
      241 agccgctatt tcatgatttt ttcgtaggct ttggaggcct attttctaga cctggtggtt
      301 cttttttctc tttcgtgggt ttgcgagttg gtttggccgg gctggccttg gtaggtgtgg
      361 gtgtcggctt tttgccgcta ttttgcaggg ttggcacggg tgtgcgcgag ggtcgaacag
      421 ttagggtggg tgcagcctcc gtctttgcag acaaaggtgt cgacaacggt gcaacggtaa
      481 cgactggcgc tgttgtaggt acaggtgtct tggtgggtac tggcgtcggg ggaacgggag
      541 taatggttat cagcgtaggc gttatggttg gcagtggtgt gcctcccatg agagcttcga
      601 tattttgcgc ctcgctcttg actagctctg ccatttgcat cagccgttcg atttcggagc
      661 gactatctgg gggagccgct tgagagagaa caggcataag ggaaatttgt ccttctacga
      721 cattccttaa tttattagcc aattccctgg ctagcaaggt gtttgtcgta cccagcgacc
      781 gcaacaaacg ggacgcctca ttcgcttgag aataaaattg atcgacggta ttgtgcaaat
      841 attcataacg gccttcgata accaggcttt gtacctccac caatctgcgc tctgcaaatg
      901 tgacataata ttgagcgcgg cgggcttcgc tgaaggacaa tgccagttgg atctgctcaa
      961 ggctcatctt aaccccatag agtgtgtccc ctgggattga atgttgggct gcggcggcta
     1021 caccactggt tgtcgaaatc aaaaatacca ataatatcgt cgcaacgaca aactggtaag
     1081 caaagcgttt ctgggcaaag agagataaga gtgtattcca aacgcttgca cccactgtac
     1141 gtagcgattt tggcgtactg gcagcttttt cctgctcgat gcgggccagc aaccgctgcc
     1201 gagaggcggc aataaactcg ggccgtggag ccagggcatc tctgcgctct tgcagccagg
     1261 tagcggtttc caggagcgag cttaactcat ccgcatggtc gggatagcga gcaagcgcgg
     1321 aatccaccga ctcttcaccg ctgcggatca gctccaggca agtatgcaga atttgctcaa
     1381 tcccttcgcg cttttcggcc atcccgttta cctttgctct tctcaaattt tgtccgcaag
     1441 gccgccaggg cacggtgttg caagaccctc acagccccag ccgaacgacc cattgcatga
     1501 gctgtctctt caggactcat atcactcaaa aatcgcatga ccaatacatt gcggtaatca
     1561 tctttcagat catccaaggc ttcgcgtagt tccttgtgct ccatctgctg gctgacgacc
     1621 tcacccggtc cgggcttgtg atcagcgatc atcacgtctt ccgaagacaa ctgacctgcc
     1681 gttcgtcgtt cacgacggta atgatcaatc agcgcattg
//
LOCUS       DBNHOH010000781         9688 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1075110, whole genome shotgun sequence.
ACCESSION   DBNHOH010000781 DBNHOH010000000
VERSION     DBNHOH010000781.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9688)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9688)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9688
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1075110"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="AC16V7_13970"
     CDS             <1..195
                     /locus_tag="AC16V7_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258276.1"
                     /translation="PSATQQVQATLFPDQATQPGLFPTEAGIPGSASTALPGATVSTL
                     APPTPILATLTPWPTNTGAP"
     gene            288..1130
                     /locus_tag="AC16V7_13975"
     CDS             288..1130
                     /locus_tag="AC16V7_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258277.1"
                     /translation="MNTDQKDTGGLPIRSNLTLAYIISLVIAALMTIASLAGLLYQTV
                     IYPTDELVQSFVPNDVVNLLIGLPTLLGAMWLTRRGRLIGLLLWPGALFYVFYTYIIY
                     TYSMPLNAGLLMHLALVLLSTYSMIGLVSSIDGKAVQNRLSGAVPEKAGGGVLVGLGV
                     LFLIRVIGVLVNAIINQTPLAATELALNISDFLITPTWIIGGVLLWRRNTPGYVFGLG
                     LLFQASMAFVGLIFVLILRPLITTAQFSLIDVLIVIVMGMICFVPFGLFMRGAASDRA
                     PSSV"
     gene            complement(1275..3575)
                     /locus_tag="AC16V7_13980"
     CDS             complement(1275..3575)
                     /locus_tag="AC16V7_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258278.1"
                     /translation="MDLHFLPLVRHEGQDQAELPGLHIAVPPRRMARGRQSDRLILYF
                     TMEGNAPLVAEQQEQLIRRLVEVYYKTPGSVTAALRNVIENLNQYLLDRNLRAASTGR
                     QGLGLLTAITLRDDRLILAQSGSVAAYLLTADEVQHIVDLEPTRRGLGLGRTAPLSYA
                     QMSLSDNDVLLVSHRPPAAWNENLLTGLHGQGLESLRRHLVPAAGDELAAVLIQARPG
                     PGKTFLLHPKPLPISAEQVTPQPSEVIEEAVPAIEETPTVDEPLEMSPPPEAVTWDAP
                     PADVPPMQAAPALEDELISPPSTTPPPVSPTYPEEETPELVSPVRGARRRTRRSLALP
                     VLATISRAFGTAFSALSQSLSTLFKRMLPGEGSLPAPVMLLTAVIVPLIVVAVATVIY
                     FQSGVAGQYQGLYAQAVQAAGAAQAENDPLARERAWQTVLEKLDQADAYQVTSDTQAL
                     RDTALQAIDELNLAIRLDFQPAIIEGLPSGSQVTRIVATETDLYLLDSSTGVVVRAIF
                     AGRGYDIDKTYQCGPGAQGSQGIGPLVDIHLYPKPDGSGTALLGMDGQGNLLECSPGA
                     PPLFTLLASPPAGWAGPNAFTIHAGDAYVLDPGTKQVWVYRNGSFSTPPDLFFDQDIP
                     PLEDAIDLAAGQEDLFLLHQDGHITLCSIGSLGVVATQCTDPQPYADARPGRSGLPLT
                     PPAPFIEVRSTQPPDPSLYLLEAGTPGVYHFSLRLAYQRQLRLQMNLVTRDILETKSA
                     TAFALSPDGRVMFLAFGDEVLYAGMP"
     gene            3696..4718
                     /locus_tag="AC16V7_13985"
     CDS             3696..4718
                     /locus_tag="AC16V7_13985"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6258279.1"
                     /translation="MRLHIVRSVEDFDSLAEEWNNLLSQSASHVPFLRHEYLATWWRT
                     LGGGEWSDGELYIVTARHADDALCGIAPLFFTKNLDGEAALMLLGSIEISDYLDVIAR
                     PEDLPSFVNTLLEHLASPEAPAWQALDWYNLLESSPTLPVLKSAVSRLGWQLNVERLH
                     HCPFIPLPGDWEIYLAGIDKKQRHEIRRKIRRAENADIPVRWRIVTQEDNLDAEIDAF
                     LDVMGQDPQKQAFLTDAMRLQMRLTIKAAQRAGWLQLSFLEVDGDIAAGYLNFDYANR
                     IWVYNSGINFEYSSYSPGWVLLGYLLQWANEQQRGAFDFMRGDEDYKYRFGGVDRFVV
                     RAKITR"
     gene            complement(4731..5840)
                     /gene="amrS"
                     /locus_tag="AC16V7_13990"
     CDS             complement(4731..5840)
                     /gene="amrS"
                     /locus_tag="AC16V7_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HIR6258280.1"
                     /translation="MSSLADVLDSMTVEGTLYERLPDDSVRCVACGHRCLIRPGRRGI
                     CQVRFNQGGTLRVPWGYVAALQADPIEKKPFFHVLPGSDALTFGMLGCDFHCGYCQNW
                     LTSQVMRDSASDVSISYVRRVMPDQMVEYARHSGASVVASSYNEPLITSDWAVDIFKK
                     ARQAGMQCVYISNGNVTPEVLEYIRPYIVGYKIDLKTMQDKRYRQLGGVLDNVLEGIR
                     LAHGKGLWVEVVTLVVPGFNDSSEELMDAAQFIRSISPDIPWHVTAFHPDYKMTDPLA
                     TSVRTLLRAADIGIEAGLNFVYAGNLPAHVGDYENTYCPKCRELLIKRSGYIIHEYRL
                     TAQGTCLRCDTAIPGVWTEHPEQVHIGGWGMPRRI"
     gene            6043..6648
                     /gene="mobA"
                     /locus_tag="AC16V7_13995"
     CDS             6043..6648
                     /gene="mobA"
                     /locus_tag="AC16V7_13995"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HIR6258281.1"
                     /translation="MLSLVIQAGGESRRMGKDKALMPFLGKTLIERVIERLSGIADEL
                     LVTTNKPESYAFLGLPLAQDLIPGRGALGGLYTALSAASYPLVAVVACDMPFVSASLL
                     TFQRDLLLQLGFDAVIPRTAGGTEPFHAIYDRASCLPAVKAAIQADKWRVDAWFARAN
                     VRLLAPEETHPHDPQGLAFWNVNTPEDLAEAETIAMAEKGD"
     gene            complement(6678..7343)
                     /locus_tag="AC16V7_14000"
     CDS             complement(6678..7343)
                     /locus_tag="AC16V7_14000"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6258282.1"
                     /translation="MLHNFGVLWDMDGVLVDTAEFHFRSWEQTLREMDIPFERRKFQA
                     VFGRNNYDTLTSLLHRPPEAELLQAVSEQKESLFRKLIHGKAIPLPGVRTWLEHLRHL
                     GALQAVASSAPQENIDLLVDELGLHSYFATLCAGHMLPSKPDPALFLLAASILGLSPR
                     VCIVIEDSLAGVEAAHRAGMKCLAVASTNPVGALGQADLVLERLDQMGVDEALSRLGV
                     ETA"
     gene            7521..9359
                     /locus_tag="AC16V7_14005"
     CDS             7521..9359
                     /locus_tag="AC16V7_14005"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR6258283.1"
                     /translation="MFTRLWQLLVGSPLPTQELAHKRLNKIRALAAFSPDALSSIAYA
                     NQEIFLGLVVAGSAGLIFSFPISATIALLLCIVAASYYQTIHGYPSGGGSYIVARHNL
                     GTLPGLVAGAALMIDYVLTAAVSLTAGVEAMASAIPELWPYRMQAALFILAVITLINL
                     RGTQESGTAMALPVYGFLIIYIIMILYGIYQAIIQGPGTLVSTAPPASQPLTIFLVLH
                     AFASGSTALTGIEAISNGVPAFKPPEARNAGRTLIAMALLMGFLFVGSMGLTQYFAVV
                     AGPQETILSALARRLLGNGILYYLVQASTLLILAVAANTSFTGFPRLASIMARDGFLP
                     RPLYNLGERLVFSNGMLVLAAVAGVLIIAFGGDSHSLVPLFAVGAFLAFTMSQAGMVV
                     HWLRERGAGWWLKSAINGTGALVTATTLGIVAISKFVHGAWFTLVLIPSLVYIFMKIK
                     GHYTEVARELTLRGLPPSLRPAPKMRLVVPISGVHRGVINAVNYARSISENITAVYIE
                     IDPGSGEKIQERWNEWFPDLSLVIRPSPYRSTIRSLLDFLDSYDIESNDGQLAAVVLP
                     EIVPAKNWQTLLHNQTAWLIKAALLYRRRHLGYQRVIIDVPFHLKK"
     gene            9374..9664
                     /locus_tag="AC16V7_14010"
     CDS             9374..9664
                     /locus_tag="AC16V7_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR6258284.1"
                     /translation="MKILALEQDVPGVTSEQFQPYLKTEAAKVWELNQAGVIRETYFR
                     QDRTSAILVLECADEEDARRILGTFPLVRAGLISFELIPLIPYPGFSRLFGE"
BASE COUNT         2057 a         2864 c         2709 g         2058 t
ORIGIN      
        1 ccatcggcaa cccagcaggt ccaggcgacc ctcttcccgg atcaagccac gcagccgggt
       61 ctcttcccaa ccgaggcagg cattcctggc agcgcatcga cagccctccc aggcgctact
      121 gtctccaccc tagccccccc aacacccatc ttagccacgc tcaccccttg gccgaccaac
      181 accggcgcgc cgtaagacgc gcgaggtgcg aaacacgctg ccacagtcct aggctgatag
      241 gggttactca tacagcagca acctgactat aaggaactgg ataatagatg aacacagatc
      301 aaaaggatac tggcggtctg ccgatccgaa gtaacctcac gctcgcatac attatctcgc
      361 tggtgatcgc cgctctcatg acgattgcgt ccctagccgg tctcttgtac cagactgtca
      421 tctaccccac ggacgaattg gtccagtcat ttgtgcccaa tgatgtagtt aatctcctga
      481 tcggtctgcc taccctgctt ggtgcgatgt ggctaacgcg gcgcggcagg ctcatcgggc
      541 tgctcttgtg gccgggcgcg ctattctacg tgttttacac ctacatcatt tacacctaca
      601 gcatgccgct caatgcaggg ctcttaatgc accttgcgct ggtcttgttg agcacgtatt
      661 cgatgatcgg tcttgtctcc agcatcgatg gaaaagcggt acaaaatcgg cttagcggcg
      721 ctgtaccaga aaaagctggc gggggcgtcc tggttgggct tggtgtcctg tttcttatac
      781 gggtcatcgg cgttttggtt aatgccatca tcaaccagac tccgctcgcc gcgacggagc
      841 tggcgcttaa tatctcggac tttttgatca ccccgacctg gattatcggt ggagtgctgc
      901 tttggcggcg caatacgcct gggtatgttt tcggcctagg gttactcttt caggcaagca
      961 tggcatttgt cggattgatc ttcgtattga tcttacgccc gctcataacc acggcgcagt
     1021 tctcgctaat cgatgtcctt atagttattg tcatgggcat gatctgtttc gtcccttttg
     1081 gtcttttcat gcgcggcgcg gcatctgacc gcgccccatc gtccgtgtag cttagaatag
     1141 cgagagggca gcaaaaccac tggctgaggg tagcatgata ggaaaaagcc tgaagtgatc
     1201 ctggctagag acgacaagat gctcacaaac acgccaatag caggatggat ataaatatat
     1261 tagactaggg aaatctacgg catcccggcg tagagcacct catccccaaa agccaggaac
     1321 atgacccttc catctgggct gagggcaaaa gcggtcgcac ttttggtctc caggatatcg
     1381 cgggtgacca ggttcatctg tagcctgagc tggcgctgat acgccaggcg caagctgaag
     1441 tgatataccc ccggcgtccc cgcctcaagc aggtagagcg atgggtcggg cggttgggtt
     1501 gaacgcacct caataaacgg tgcaggcggg gtcaagggca gacctgagcg gccggggcgg
     1561 gcatccgcgt agggctgagg atcggtgcac tgcgtggcga cgacacccag gctaccgatg
     1621 gaacacagag tgatgtgccc atcttggtgc agcaagaaca ggtcttcctg gccggctgcc
     1681 aagtcaatcg catcctctag cggtgggatg tcttgatcga aaaacaggtc aggcggcgta
     1741 gaaaaactgc cgttgcggta aacccacacc tgcttcgtgc ctggatcaag cacataggca
     1801 tccccagcat ggatggtgaa cgcgtttggc cctgcccagc cggcaggagg ggaggccagc
     1861 aaagtgaata aggggggagc gccggggcta cactccaaaa ggtttccctg cccgtccatg
     1921 cctagcaggg cagtgcctga accatctggt tttggataca ggtgaatatc caccaacgga
     1981 ccgatgcctt gactaccctg cgcgcctggc ccgcactggt aggtcttatc gatatcataa
     2041 ccgcgcccgg cgaagatcgc cctgaccaca acccctgtac tggagtcgag caggtacaag
     2101 tcggtctcgg tggcgacgat gcgcgtgact tgtgatccgc tgggcaggcc ttcgatgatg
     2161 gcgggctgaa aatccagccg tatggccaga ttcagctcat cgatggcttg cagagccgta
     2221 tcccgcaaag cctgggtatc actcgtcacc tggtacgcat ccgcctggtc gagcttttcc
     2281 agcacggttt gccaggctcg ctccctagct aacggatcgt tttccgcttg agcggcaccc
     2341 gccgcctgca cagcctgggc gtacagaccc tggtattgcc cggcaacccc gctttgaaaa
     2401 taaattaccg tagcaaccgc caccactatg agcggtacaa tcacggcggt aagcagcatc
     2461 actggagctg gcaggctacc ctctccaggc agcatgcgct taaaaagcgt gctcaacgat
     2521 tgggagagcg ccgaaaatgc cgtcccaaaa gctcggctga tcgtcgccag gacaggcagc
     2581 gccaagctgc gccgcgtccg gcggcgagcc ccccttaccg ggctgaccag ttcgggggtc
     2641 tcttcttccg ggtaggttgg ggaaacaggt gggggagtgg tagagggggg cgaaattagc
     2701 tcgtcctcta gagctggagc cgcctgcatg gggggcacat cggcgggtgg tgcatcccaa
     2761 gtcacggctt cgggaggggg agacatctca agcggctcat ccactgtggg tgtctcttca
     2821 atggctggca cagcctcctc gataacctca gatggttgag gagtaacttg ttccgctgaa
     2881 atcggcaggg gtttcgggtg cagcaggaag gttttgcccg gccctggcct ggcctgtatc
     2941 aacactgcag ccagttcatc ccctgccgca gggactagat ggcggcgcag gctctcaagc
     3001 ccttgaccgt gcaagcccgt cagcaggttt tcattccacg ccgcaggcgg ccggtggctg
     3061 accagaagca catcgttatc actcaggctc atctgggcat aggagagcgg cgcagtgcgt
     3121 cccaatccca gaccgcgccg ggtgggttct agatcaacga tatgctgcac ctcatccgcg
     3181 gtaagcagat aggcagcgac tgaccccgat tgagcgagga tgagccggtc gtcgcgcaag
     3241 gtgatcgcgg tcagcaaccc caatccctgg cgaccggtgc tggcagcgcg caggttacgg
     3301 tcgagtaagt actggttcag attctcaatg acgttgcgta atgccgcagt caccgagcca
     3361 ggcgtcttgt aatagacttc caccaggcgt ctaatgagct gctcttgctg ctccgccacc
     3421 aggggggcat tgccctccat cgtaaagtaa aggatcagcc ggtcggattg ccggccgcgc
     3481 gccatacggc gaggtgggac agcgatatgc aatccgggca attcagcctg atcttgccct
     3541 tcatgacgaa cgagaggaag aaaatgaaga tccatatacg gtgcagcctg cctatggaca
     3601 ttatactggt aaaatgcaac tggtgagcgc ttacccacta aatttggagc tatggttctt
     3661 ctcaatcctg agggttgaga aaacacggag attttatgcg actccacatc gtccgcagcg
     3721 tggaagattt cgatagcctg gctgaggagt ggaacaatct actatcccag agtgcaagtc
     3781 acgtgccatt tttgcgtcac gaatatctgg ctacctggtg gcgcaccctg ggcggtggtg
     3841 aatggtctga tggagagcta tatattgtca ctgcccgcca cgcggacgat gccctttgcg
     3901 ggatcgcgcc gctctttttc accaagaact tggatggaga ggctgcactg atgctgttgg
     3961 gcagcatcga gatctcagac tacctggatg tgatcgcccg ccctgaagac ctgcctagct
     4021 tcgtcaacac cttgctggag cacctcgcca gccctgaagc acctgcctgg caagccctgg
     4081 actggtacaa cctgctggaa agctcgccca ccctaccagt gctcaagtcc gctgtatccc
     4141 gcctgggctg gcagttgaac gtagaacgcc tgcaccactg ccccttcata cccttgccag
     4201 gggactggga gatttacctg gcaggcatcg acaaaaaaca gcgtcacgag atccgccgca
     4261 agattcgccg cgccgaaaat gccgacatcc ccgtacgctg gcgaattgtg acccaagaag
     4321 acaacctgga cgccgagatc gatgctttcc tagatgttat ggggcaggac cctcaaaaac
     4381 aagccttttt gactgacgcc atgcgcttgc aaatgcgcct gacaatcaaa gctgcccagc
     4441 gagctggctg gctgcaattg tcttttttag aagtcgatgg ggatattgcg gctggttatc
     4501 tgaacttcga ttacgctaac cgaatctggg tctacaactc ggggatcaat ttcgaataca
     4561 gctcctactc acctggctgg gttctgctgg gctacctgct gcaatgggca aacgagcagc
     4621 agcgaggagc ctttgacttc atgcgcggcg acgaagatta taagtaccgc tttgggggcg
     4681 tcgaccggtt tgtcgtgcgc gccaaaatta cccgctaata agcgtaagat ttatatccgc
     4741 cttggcatcc cccacccgcc gatatgcacc tgctcggggt gctctgtcca cacgcctggg
     4801 atcgctgtat cgcatctcaa acaggttcct tgggcggtta ggcgatactc atggatgatg
     4861 tagccggagc gctttatcag cagctcgcgg cacttagggc agtaggtgtt ctcgtaatcg
     4921 cccacatggg cgggcaagtt gccagcgtag acaaaattca ggcctgcttc tatcccgatg
     4981 tccgccgccc gcagcagagt gcgtacggag gtagccaatg gatcggtcat cttgtaatcg
     5041 ggatgaaaag cggtgacatg ccaggggata tccggtgaga tggagcggat gaattgcgcc
     5101 gcgtccatca gctcctcgct actgtcgttg aagccgggca ccaccagagt gaccacctcc
     5161 acccaaaggc cctttccatg ggccaggcgg ataccctcca gcacgttatc cagcacaccg
     5221 cccagttgcc ggtagcgttt gtcctgcatg gtttttaaat cgattttgta cccaactatg
     5281 tacgggcgga tatactccag cacttcgggg gtgacgttgc cattggagat gtacacgcat
     5341 tgcatcccag cctgcctggc cttcttgaag atatccaccg cccagtccga ggtgatcagc
     5401 ggctcgttgt acgatgaggc gaccacagat gcgccgctgt gacgggcgta ttccaccatt
     5461 tgatcgggca tgacgcgccg cacatagctt atggagacat ccgatgccga gtcacgcatc
     5521 acctgagagg tcagccagtt ttgacagtac ccgcagtgga aatcacatcc cagcatgcca
     5581 aaggtcagcg catcgctacc cggcaggacg tggaagaatg gctttttctc gatcggatcg
     5641 gcctgtaatg cggccacata gccccagggc acacgcaatg tccccccctg gttaaagcgc
     5701 acctggcaaa tgccgcgccg cccaggtcgg atcaggcagc gatgcccaca ggcaacacag
     5761 cgcacggaat catcgggcag gcgctcgtag agcgtgccct ctacggtcat ggaatctaaa
     5821 acatcagcta aggaggacat cttacacgct cctgtttcta ccagtaatta tgcagctgca
     5881 gagacaaagg ggaggagccc cctttacctt aaccgcacaa gtcctatctg gcataagtat
     5941 aacaggactt gcacatttaa aaaaagtcca ggggaagaga caggcaaaac ctgtccattc
     6001 ccctggacta ccccaacggc acatcttctg tataatatca atatgctcag cctggtgatt
     6061 caagctggcg gcgaatcacg ccgcatggga aaagacaagg cgctgatgcc ttttctaggg
     6121 aagaccttga tcgagcgcgt gatcgaacgt ctctccggca tagcagatga gctactggtc
     6181 accaccaata agcccgagag ctacgccttt ttagggttgc cgctcgcgca ggatttgatc
     6241 ccagggcgag gcgccctggg cgggctgtac actgcactga gcgcggcgag ctacccgctg
     6301 gtggctgtag ttgcctgcga catgcccttc gtcagcgcga gtttgctaac cttccagcgc
     6361 gacctgctcc tgcagcttgg ttttgacgcc gtcatcccgc gcacggctgg tggcacagag
     6421 ccattccatg ctatctatga tcgtgctagc tgcttgccag cggtaaaggc tgccatccaa
     6481 gccgataagt ggcgcgtcga tgcctggttc gccagggcga atgtgcgctt gctggcaccc
     6541 gaagagaccc acccgcacga cccgcagggt ctggccttct ggaacgtcaa caccccagaa
     6601 gacctagccg aagcagagac gatagcaatg gcggaaaaag gggactaaag aactgttccc
     6661 gctgcaaaaa tcctaactta cgcggtctca acgcccagcc tgctcagcgc ctcatccacc
     6721 cccatctgat ccaatcgttc taggaccaga tccgcctggc ccagtgctcc caccgggttg
     6781 gtgctggcaa ccgcaagaca tttcatgccc gcccgatggg cagcctctac ccccgccagc
     6841 gagtcctcga tgacaataca cacccgcggc gaaagtccca atatgctcgc agcgagcaaa
     6901 aacaaggctg ggtccggctt gctcggcagc atgtggccag cgcacaaggt cgcgaaatag
     6961 gagtgtaacc ccagctcatc caccagcaga tcgatgttct cttggggagc cgaggaggcc
     7021 accgcctgta aagctcccag gtggcgcagg tgctccagcc atgtccgcac gcctggcagg
     7081 ggtatagctt tcccatggat cagctttcgg aacagggact ccttttgctc gctgaccgct
     7141 tgtaaaagct ctgcttcagg agggcgatgg agaaggctgg tcagcgtatc atagttgttg
     7201 cgcccaaata ctgcctggaa ctttctccgc tcaaagggga tatccatctc tctcaaggtc
     7261 tgttcccacg agcggaagtg aaactcggcg gtatccacca gcacgccatc catgtcccaa
     7321 agcacgccaa aattgtgtaa catcttttca ggcccttagc gtaataaaga cttggcaggg
     7381 attggttggg tttttcatcc cagctacaat caattatatc gatcaacggt ggcataggca
     7441 tcccacagga tgacaccccc gcagaaacac aaaagcggag tacacttaag catcaaatcc
     7501 tatctggcac aggagcgaaa ttgtttacac gcctttggca acttctggtg ggctcgccgc
     7561 tgccaaccca ggagctggct cataaacggc tgaacaagat tcgggcgcta gctgctttct
     7621 cgccggatgc cctgtcgtcc atcgcctacg ccaatcagga gatctttctg gggttggtgg
     7681 tggcaggcag cgcagggctg atcttctctt tccccatcag tgccaccatc gccctgctgc
     7741 tgtgtattgt ggcggcctcc tattaccaga ctatccacgg ctacccgtcc ggcgggggtt
     7801 cctatatcgt cgcccggcat aatctgggaa cacttcccgg gctggtggcg ggggcggccc
     7861 tgatgatcga ttatgtgctc acggcggcgg tcagcctgac ggcgggcgtg gaggccatgg
     7921 cttccgccat acctgagttg tggccctacc ggatgcaggc ggctctgttt atactagccg
     7981 tgatcaccct gatcaacctg cgcggcaccc aggaatctgg tacagccatg gcgctgccgg
     8041 tttacgggtt cttgatcata tacatcatca tgatcctgta cggcatctac caggccatca
     8101 tccagggacc gggcacgctg gtaagtactg cgccgcccgc ctcccaaccg ctgacgatct
     8161 tcctggtgct gcacgccttc gcctccggca gcaccgctct gaccggcatc gaggccatct
     8221 ccaacggcgt accagccttc aaaccgccag aggcgcgcaa cgccgggcgc acactgatcg
     8281 ccatggcctt gctgatgggc tttctcttcg tgggaagcat ggggctcaca caatacttcg
     8341 ccgtggtagc cgggccacag gagactatcc tctccgccct ggcgcgtcgc ctcttaggga
     8401 atggcattct atattacttg gtgcaagcca gcaccctgct catcctggct gtggctgcca
     8461 ataccagctt taccggcttc ccgcgcctgg cttccatcat ggcgcgggat ggctttctgc
     8521 ctcgcccgct gtacaacctg ggcgagcggc tggtattctc gaatggcatg ttggtgctgg
     8581 ctgctgtcgc cggtgtgctg attatagcct tcggagggga ttcgcactcc ctggtgccac
     8641 tgttcgctgt gggggcattt ctggctttta ccatgtccca ggctggcatg gtggtgcact
     8701 ggctgcgcga gcgtggcgca ggctggtggc taaagagcgc aatcaacggg accggcgcgc
     8761 tggtcacagc gacgacgtta ggcattgttg ctatcagtaa gttcgtacac ggtgcctggt
     8821 tcaccctggt cttaatcccc tcgttggtct acatttttat gaaaatcaaa ggccactata
     8881 ctgaagtagc ccgcgagctg accctgcgcg gccttccgcc ctccttgcgc ccggcgccca
     8941 agatgcgcct ggtcgtcccc atctcagggg tgcaccgggg agtcatcaat gcagtcaact
     9001 acgcccgctc gatttccgaa aacattaccg ccgtctatat cgaaatcgat cccggaagcg
     9061 gagagaagat ccaggagcga tggaatgaat ggttccccga cctaagcttg gtcatacggc
     9121 cctcaccata tcgctcgacc atcagatcat tgctggattt tctcgacagt tacgacattg
     9181 agagcaatga cgggcaactg gctgccgtgg tgctgccaga gattgtccct gccaaaaact
     9241 ggcagactct tttacacaac cagaccgcct ggctgatcaa agccgccctg ctctaccgcc
     9301 ggcggcacct ggggtaccaa cgtgtgatca tcgacgtgcc gtttcacctg aaaaaataga
     9361 ttaggagcct agtatgaaaa ttctcgccct cgagcaggat gtacctggtg tgaccagcga
     9421 gcaattccaa ccctacctta aaactgaggc tgccaaggtg tgggagctca accaggcagg
     9481 cgtgatccgc gaaacttact ttcgccagga ccggacaagc gctatcctgg tgctcgaatg
     9541 cgccgatgag gaagacgcca ggcgcatcct gggcaccttc ccgctggtgc gggcagggtt
     9601 aatctctttc gagctgatcc cactcatccc ctatccgggg ttctctaggt tgtttggcga
     9661 gtagtattgt caaccggcaa atccggtt
//
LOCUS       DBNHOH010000782         2351 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_497715, whole genome shotgun sequence.
ACCESSION   DBNHOH010000782 DBNHOH010000000
VERSION     DBNHOH010000782.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_497715"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="AC16V7_14015"
     CDS             <1..1138
                     /locus_tag="AC16V7_14015"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6258285.1"
                     /translation="VGSWMQTTAQGYLIFELTQSPAYLGYVGFAAGIPSWLFTLYGGL
                     IADRMPRRNLLVMTQSAMMVLAFGLATLTFAGIVQPGHILGFAFCLGIANAFDAPARQ
                     SFVLEMVSREDLTNAIALNSSMFTAATVVGPAVGGLLYAALGPAWCFTINGASFMAVI
                     TALLLMRLKPASHTPRHGSALADVAAGIGYVRKNVIPRTVISHLAMVSMFGISFVSLM
                     PAWAVRVLGGDATTNGFLQSARGVGALSGALMIAAFGGYIVRGRLLTLGGFILPLTLL
                     AFSAVRWLPLSLLTLVGVGWGFMVFVNSSNALLQSHVPDDLRGRVMSIYTLAFFGLMP
                     VGALLAGGAATWVGEPLTVALAASMLLVFGALVWIKVPQLRSSD"
     gene            complement(1333..>2351)
                     /locus_tag="AC16V7_14020"
     CDS             complement(1333..>2351)
                     /locus_tag="AC16V7_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR6258286.1"
                     /translation="KRRTGNGKTLVVVGARENNLKNIDVRIPLGRLVCITGVSGSGKS
                     TLMVEILYKALARHLHGARAQPGDHDRVEGLENIDKIINIDQSPIGRTPRSNPGTYTG
                     LFDAIRALFAELPESKIRGYKPGRFSFNVHGGRCEACQGQGQLRIEMQFLPDVYVPCD
                     VCHGARFNRETLQVRFKEYNIADVLDMTVDHALEIFEAFPGMVNKLKTLQEVGLGYIR
                     IGQPAPTLSGGEAQRVKLSRELSRRATGRTMYVLDEPSVGLHAADVHKLIEVMQRLVD
                     AGNSVLIIEHNLDTIKVADYLIDLGPEGGDGGGLVVAEGTPEQVCERDDSYTGQYLRP
                     FICK"
BASE COUNT          442 a          643 c          676 g          590 t
ORIGIN      
        1 ggtcggcagc tggatgcaga ctactgcgca aggttactta atctttgagc tgacccaatc
       61 accagcctat ttgggctatg tggggttcgc tgcggggatc ccctcctggc tgtttacctt
      121 gtacggcggc ttgattgctg atcgtatgcc ccgccgcaat ctgctggtga tgacccagtc
      181 agcgatgatg gttctggcgt ttggtctggc cacgctgacc ttcgctggca tcgtgcagcc
      241 ggggcatatc ctgggttttg ctttctgctt gggaatcgct aacgcctttg atgcgccagc
      301 caggcaatct tttgtgttgg agatggtctc gcgggaagat ctgaccaatg ccatcgcctt
      361 aaattcaagc atgttcaccg ctgcaaccgt ggttgggccg gcagtgggcg ggctgctgta
      421 tgctgccctt ggtccagcct ggtgcttcac catcaatgga gcttctttta tggcagtgat
      481 tactgcctta ttactcatgc ggttaaaacc agcttcacac accccacgac atggctcggc
      541 cttggcagat gtggccgcgg gcatcggcta tgtccgcaag aatgtaatcc cccgaacggt
      601 gatcagtcac ttggctatgg tcagcatgtt tgggatcagc tttgtttcct tgatgcccgc
      661 ctgggcggtg agagtgctgg gtggtgatgc caccaccaac ggtttcctcc aatcggcgcg
      721 aggtgtggga gcgctgagcg gggcattgat gatcgcggca tttggtggct atattgtgcg
      781 cgggagattg cttaccctgg gcggcttcat cttgccgctc accttgttgg cgttctccgc
      841 ggtgcgctgg cttccgctat ccttgttgac actggttggc gttggctggg ggttcatggt
      901 gttcgtcaac tcatccaatg ccctgttgca aagccacgtg ccagacgatc tgcgcggccg
      961 ggtgatgtcc atatacaccc tggcattctt tggattgatg cctgttggcg ccctgctagc
     1021 tggcggcgct gccacctggg ttggagagcc gctcacggta gctttggctg cctcgatgct
     1081 gttggttttc ggcgctttgg tttggatcaa ggtaccccaa ctgcgtagtt cagattaatt
     1141 gatattgagg agaagagaat gggatattag ctattgggta aaagcggact gagagtatct
     1201 gaactggctt taggcaccat gacttttggt gaagattggg gctggggcgc ctctaaagaa
     1261 gagtccaaaa atggctaaaa tattgctatc gcggctgtgt gcgcccgata ccgtatcgtg
     1321 tccttctccc tatcacttac aaataaacgg tctcagatat tgaccggtgt acgaatcatc
     1381 ccgctcgcac acttgctctg gcgtgccctc tgcaaccacc aatccgccgc catcaccacc
     1441 ttcgggtcca aggtcgatca agtaatcagc gactttgatc gtgtcgagat tatgctcgat
     1501 gatcaacacg ctgttgccag catcgactaa acgctgcatc acttcaatca gcttgtgcac
     1561 atcagcagca tgcaggccaa ccgaaggctc atccagtacg tacatggtgc gcccggtagc
     1621 gcggcgtgaa agctctcgag acagcttaac gcgctgcgcc tcgcctcctg aaagcgtggg
     1681 ggctggctgc ccaatgcgga tatatcctaa gcccacttct tgcagggttt tgagcttgtt
     1741 caccattcca gggaaggctt cgaagatttc cagggcatgg tcaacagtca tatccagcac
     1801 gtcggcaata ttgtattctt tgaagcgcac ttgcaaggtc tcgcggttga agcgcgcccc
     1861 atgacacacg tcacaaggca cgtatacatc gggcaggaac tgcatctcga tgcgcagctg
     1921 accttgcccc tgacaggcct cacagcgccc gccatgcaca ttgaaggaaa agcgcccggg
     1981 cttgtagccg cggatcttgc tctcgggtag ctcggcgaat agggcgcgga tggcgtcgaa
     2041 caaaccggta tacgtgcccg ggttggagcg cggcgtgcga ccgatgggcg actgatcgat
     2101 gttgatgatc ttgtcgatgt tttccagacc ctcaacccgg tcgtggtcgc cgggttgcgc
     2161 acgcgctcca tgtaaatgcc gtgccagggc tttgtacagg atttccacca tcagggtgga
     2221 tttaccagag cctgagacgc cagtgatgca caccagtctg cccagcggga tgcgcacgtc
     2281 aatgtttttt aggttgttct cgcgcgcccc aactaccacc agcgtcttgc cattgccggt
     2341 gcgccgttta t
//
LOCUS       DBNHOH010000783         1294 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1331002, whole genome shotgun sequence.
ACCESSION   DBNHOH010000783 DBNHOH010000000
VERSION     DBNHOH010000783.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1331002"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="AC16V7_14025"
     CDS             <1..450
                     /locus_tag="AC16V7_14025"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR6258287.1"
                     /translation="QEATKMERNFEERVRTGFKKQGFMTAIGGELVEVQPGFCRIHLP
                     YSEKVAQQHGFFHGGVISALADNAAGFAGYSLMNSDQQPLSVEFKINFLAPAVGDTLE
                     ARGRVIRSGYRIKHVNVEIFALDQGNEQLVAVALANIASSTGVQEIS"
     gene            701..1078
                     /locus_tag="AC16V7_14030"
     CDS             701..1078
                     /locus_tag="AC16V7_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015186308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR6258288.1"
                     /translation="MANTLNTPEDLLPLTPAVFNIMLALADGEKHGYGIMLEVEANTD
                     GQITMGPGTLYGSIKRMLKAGLIEECDERADPKMDDQRRRYYRLTGMGQQVLRLEAER
                     LASQVQVARAKNLLADGLARSGA"
     gene            1075..>1294
                     /locus_tag="AC16V7_14035"
     CDS             1075..>1294
                     /locus_tag="AC16V7_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258289.1"
                     /translation="MITRMTHISPVRFSVGVYRLLLAAYPKAFRDEYGPHMVQVFQDL
                     SIRAYRQNGSDGILRLWAITLFDLLKSVV"
BASE COUNT          317 a          342 c          357 g          278 t
ORIGIN      
        1 caggaggcaa ccaagatgga gcgtaatttc gaggagcgcg tacgcaccgg atttaaaaag
       61 caaggcttta tgaccgcgat tggcggcgag ctggtagagg ttcagcccgg tttttgccgc
      121 atccacctgc cttactctga aaaggtggcc cagcagcacg gtttctttca tggcggggtg
      181 atctcggcgc tggccgacaa tgccgccggg tttgccggct attcgctgat gaacagcgac
      241 cagcagccat tgagcgttga gttcaagatc aatttcctcg ccccggcggt gggagacacc
      301 ctcgaagcca gggggagagt gatccgctcg ggataccgca tcaagcatgt caatgtagag
      361 atattcgcgt tagaccaggg caacgaacag ctggttgctg tcgccctggc aaacatcgca
      421 tcatccacag gcgtacagga aatttcatag caagtcctag atcattgcgt atatggggat
      481 ttcgatcaag ccaaaatttg actatgataa atcttcctaa atcagaaagc taagattagg
      541 tatcgaggca cattgatgta cccctttttc atttcacgtt gcagaaccaa tatataaatc
      601 tgacaaaaaa gacttgacaa ctcttattta attacgtact atatccataa cggatatatc
      661 tcgcgcggat atactgagtc tattcaatag gagcaatcca atggcaaaca ccctgaatac
      721 ccccgaagac ctgcttcccc tcaccccggc agtcttcaac atcatgctcg ccctggccga
      781 tggcgaaaag cacggctacg gcatcatgct ggaagtggaa gctaacaccg atggtcagat
      841 cacgatggga ccgggcacgc tgtacggttc gatcaagcgc atgctcaaag ctgggctgat
      901 cgaggaatgc gatgagcgcg ccgaccccaa gatggatgac cagcggcggc gctactaccg
      961 cctgaccggg atgggccagc aggtgctgcg gctggaagcg gaacgtttgg cttcccaggt
     1021 gcaggtggca agagccaaga acctgctggc agacggactg gcaaggagcg gggcatgatt
     1081 accaggatga cacacatctc ccccgtccgc ttttcagtcg gtgtgtaccg cctgctgctg
     1141 gctgcctacc caaaggcatt ccgggacgag tatggcccgc acatggtgca ggtttttcag
     1201 gacctgtcca tccgggcata caggcagaat gggtctgacg gtattctcag gttgtgggcc
     1261 atcacgctct tcgatctgct caaaagtgtg gtcg
//
LOCUS       DBNHOH010000784         5822 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_754535, whole genome shotgun sequence.
ACCESSION   DBNHOH010000784 DBNHOH010000000
VERSION     DBNHOH010000784.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5822)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5822)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5822
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_754535"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="AC16V7_14040"
     CDS             complement(<1..225)
                     /locus_tag="AC16V7_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR6258290.1"
                     /translation="MHVKIAASILAADFARLGKQVQEALDGGAEYIHVDVMDGHFVPN
                     LTIGPMIVSAIRPIVQQANAILDVHLMVEEP"
     gene            complement(271..990)
                     /gene="rpiA"
                     /locus_tag="AC16V7_14045"
     CDS             complement(271..990)
                     /gene="rpiA"
                     /locus_tag="AC16V7_14045"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HIR6258291.1"
                     /translation="MAKHEIQNSKYKAARRALDYVRDGMTLGLGSGSTAAHFVDLLGE
                     QIRSGSLREIRGVPTSEATANQARALSIPIISLSSSNPGGEPRLDLAIDGADEVDPSL
                     NLIKGLGRALLREKVVEIHAERLIIMVDETKMVPRLGTRGPLPVEIIPFEAETHVRWL
                     KTLGCRAELWVEEDGSPVITDNNNYLVRCWFERGISDVYSLARRLAERPGIVEHGLFL
                     DMTTLVIVAGEQSTRIIERST"
     gene            complement(1078..1491)
                     /locus_tag="AC16V7_14050"
     CDS             complement(1078..1491)
                     /locus_tag="AC16V7_14050"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6258292.1"
                     /translation="MPKRTLTLVIAIVVLSTLLVACGGTDTADGGSGGDAKRGEELYK
                     QTVIGSQAAPGCITCHSLEAGKVLIGPPHAGLATKAETAVPGMPAEDYLRESIIEPDA
                     HLVDGFSAGIMYQNYAKDLKESEINDLLAFLLTLK"
     gene            complement(1726..2115)
                     /gene="rplL"
                     /locus_tag="AC16V7_14055"
     CDS             complement(1726..2115)
                     /gene="rplL"
                     /locus_tag="AC16V7_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003523726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIR6258293.1"
                     /translation="MANIDKLVEELSKLSVLEASQLVQKLEEAWGVSAAAPVAMAAMA
                     SPAAAGAPAEAEEEKTEFDVIIKDAGPKKIEVIKTIRQLSSSLGLKEAKELAETVGGK
                     VMEAVSKDAAMDAKSKLEAAGAVIEVK"
     gene            complement(2219..2755)
                     /gene="rplJ"
                     /locus_tag="AC16V7_14060"
     CDS             complement(2219..2755)
                     /gene="rplJ"
                     /locus_tag="AC16V7_14060"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIR6258294.1"
                     /translation="MAITKKRKHEVVAQYSEWANRCQALILTEYTGITMKQLDELRKK
                     AREAGGEFHIVKNTLASLALKDQDMPLPEGFFEGSTAVGFAFTDGPALAKAISEFTRT
                     SEFIKIKGGYLGKQAMTAEDVKALAELPPLPVVRAQLLGTLLAPASKLARTLAEPARM
                     VAAVVKAYADRESAPEAA"
     gene            complement(3007..3726)
                     /gene="rplA"
                     /locus_tag="AC16V7_14065"
     CDS             complement(3007..3726)
                     /gene="rplA"
                     /locus_tag="AC16V7_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIR6258295.1"
                     /translation="MTKHGKKYLAAAAKVDKEQLYTPAEAVALAKETSISKFDGTVEI
                     HMRLGVDPRHADQQVRDVVVLPHGLGKTVRVLVFAQGEGASLAREAGADLVADDDETL
                     AKIQAGWTDFDVAIATPDMMGKVGRLGRVLGPRGLMPNPKAGTVVQAEDIPRVVNESK
                     AGRVEFRLDKTANLHVPIGKVSFEQNKLYENLAALMEAVKKARPAASKGTYIKRITLT
                     TTMGPGIKVDPNQAQAMEVLE"
     gene            complement(3777..4202)
                     /gene="rplK"
                     /locus_tag="AC16V7_14070"
     CDS             complement(3777..4202)
                     /gene="rplK"
                     /locus_tag="AC16V7_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011056150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HIR6258296.1"
                     /translation="MAKKLKTIIRLQIQAGKANPAPPIGPALAGHGINLMAFCKEYNA
                     RTSSRMGEIIPAEISIYTDGSFTFILKTSPAAVLLRKAAGVDKGSAQPNREKVGKVTR
                     AQVREIAELKMKDLNALDIEGAMRQIEGTARNMGIQIID"
     gene            complement(4312..4983)
                     /gene="nusG"
                     /locus_tag="AC16V7_14075"
     CDS             complement(4312..4983)
                     /gene="nusG"
                     /locus_tag="AC16V7_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIR6258297.1"
                     /translation="MEEKVPEEQPLMTEDEITEVETPQDELSAELLDSEESLDVPVEI
                     EDQAEDDRAWYVVHCYSGYENKVRHNLEQRIETMGMKDKIFDIVVPTEEEIEVKEGKR
                     RTVERRVFPGYILVNMILSEESWYVVRNTPGVTGFVGMGNNPTPLRPEEVAQIIRRME
                     AEAPRIKVTFKSGERVRIVDGPFNDFRGTVSEIDMERAKVRVMVNFFGRETPVELDFL
                     QVEKA"
     gene            complement(5047..5271)
                     /gene="secE"
                     /locus_tag="AC16V7_14080"
     CDS             complement(5047..5271)
                     /gene="secE"
                     /locus_tag="AC16V7_14080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HIR6258298.1"
                     /translation="MAKADKGKSIERRSSNKIQLFFRETVGELRKVSWPTWQDAWNLT
                     KIVLVVMVVMGLYLGVLDYIFTTLMALILG"
     gene            complement(5381..5456)
                     /locus_tag="AC16V7_14085"
     tRNA            complement(5381..5456)
                     /locus_tag="AC16V7_14085"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5421..5423),aa:Trp,seq:cca)
     gene            complement(5517..5684)
                     /gene="rpmG"
                     /locus_tag="AC16V7_14090"
     CDS             complement(5517..5684)
                     /gene="rpmG"
                     /locus_tag="AC16V7_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIR6258299.1"
                     /translation="MASKKDIRPVITLACTECKERTYTTEKNRRNDPGRMELNKFCPR
                     CRKHTLHRETK"
     gene            complement(5695..>5822)
                     /locus_tag="AC16V7_14095"
     CDS             complement(5695..>5822)
                     /locus_tag="AC16V7_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu C-terminal domain-related protein"
                     /protein_id="HIR6258300.1"
                     /translation="GDNVNLEVELIVPVALEQGSKFAIREGGLTVGAGVITKILD"
BASE COUNT         1257 a         1626 c         1458 g         1481 t
ORIGIN      
        1 gggctcttca accatcaaat ggacatccaa tatggcgttg gcctgctgga cgataggcct
       61 gatcgctgat acgatcattg ggccgatagt caaatttggg acgaagtgcc catccatgac
      121 gtccacatga atatattctg cccctccgtc gagggcctcc tggacctgct tgcccaagcg
      181 ggcgaaatcg gctgcgagga ttgaagcggc aatctttaca tgcatcacaa gtgaaatcat
      241 tatactggat tatggctcga aagatggaca tcaggtcgat ctttcgataa tgcgcgtgct
      301 ttgctcgccg gcgacgatca ccagggtggt catatcgagg aacaggccat gctcaacgat
      361 gcctgggcgc tcagccaggc gacgtgcaag gctatagaca tccgagatgc cacgttcaaa
      421 ccaacagcgc accaggtaat tattattgtc cgtgatcaca ggcgatccgt cttcctcaac
      481 ccaaagctct gcccggcaac ccagggtttt cagccagcgc acatgggttt ccgcttcgaa
      541 ggggatgatc tccacgggca gcgggccgcg agtgcctagc ctgggtacca ttttggtttc
      601 gtccaccata atgataagac gttcggcgtg aatctccacc accttctcgc gcagcagggc
      661 gcgtcccagg cctttgatca gattcaggct ggggtcgact tcgtccgccc catctatggc
      721 caggtcgagt cgcggctcgc cgcctgggtt cgaagatgat aaggagatga ttggaatact
      781 gagggcgcga gcctgattgg cagtggcctc ggaagtgggc acaccacgga tctcccgcag
      841 tgagccagat cgtatttgct ctcccagcag gtctacgaaa tgggctgcag tagacccgct
      901 gcccagtcca agcgtcatac catcgcgcac ataatccaat gcccggcggg ctgctttata
      961 ttttgagttt tggatttcat gctttgccac tgcaatatac ctgggatcct tgtggcatcc
     1021 ttttcccatt gagggggaga acaattgcgt tggggcggca atctttgtcc gcctagctta
     1081 ctttagggtc aacaagaaag ccaacaggtc gttgatttcg ctttctttca agtctttggc
     1141 gtagttttgg tacattattc ccgccgaaaa accatccacc aggtgagcat caggttcaat
     1201 gatagactct cttagatagt cttcagccgg catgcctggg acggctgttt ccgcttttgt
     1261 ggctagcccg gcatgggggg gaccgatcag caccttcccc gcctcgagcg aatggcaagt
     1321 gatacaaccc ggcgcggcct gcgacccgat aaccgtctgt ttatagagct cttcaccgcg
     1381 tttcgcgtct ccaccactac cgccatcagc agtatctgtg ccaccgcatg cgaccagcag
     1441 tgtactgaga actacgatag cgataaccag agtgagagta cgttttggca tttctcatac
     1501 tcctctttaa atagatcaat tgaatgttgg acttatcccc tcatacgtca aaggaaaaca
     1561 ttgtcccaag ccagagtcta ccacagcaaa tctacactcc acttagaaaa ttcccgaaat
     1621 ctaagacaat ggggttttgc gggtacttct actccacaaa accccatggg cgtgattaat
     1681 tttccctttt cagggtaata tacctgtggg atgtgatgag caaagctatt ttacttcgat
     1741 cacagcgccg gcggcttcaa gcttagactt ggcgtccata gctgcatcct tgctgacggc
     1801 ttccatgact ttgccaccaa cggtctcagc aagctccttg gcctctttca ggcccaggct
     1861 gctggagagc tgacggatgg tcttgatgac ctcgatcttc tttggaccag cgtccttgat
     1921 gatgacatca aactctgtct tctcttcctc agcctcggca ggggcacctg ctgctgcagg
     1981 agaagccata gcagccattg ccacgggcgc ggcagcggaa acaccccagg cttcctctag
     2041 tttttggacc agctgggagg cttccaatac gctgagcttg ctcagttctt caactaattt
     2101 atcgatattc gccattactt aatctcctta ttattaatga actctgataa gtgatcagca
     2161 gtccgtgatt tgtgatatgt ttcttactca atgcgatctt gcacgatgat atttatgact
     2221 aggctgcctc aggcgccgat tcgcggtcgg cgtaagcttt gacgactgcc gcgaccatgc
     2281 gggccggctc ggcaagggtg cgggccagct tgctggctgg agccagcagc gtccccaaca
     2341 actgggcacg taccacaggc aaggggggca attcggcaag cgcctttaca tcttcagcgg
     2401 tcatcgcttg tttgccgaga tagccgcctt tgatcttgat gaactcggat gtacgcgtaa
     2461 actctgatat agccttcgcc aaggcgggtc catcggtgaa agcaaaaccc actgcagtgc
     2521 tgccttcaaa gaaaccttcc ggcagaggca tgtcctgatc tttaagagcc aggctcgcca
     2581 gtgtattctt gacaatgtga aactcgccac cagcctcacg ggcttttttg cgtagttcat
     2641 ccaactgctt catggtgatg cctgtgtact cagtcaggat cagcgcctgg cagcggtttg
     2701 cccactcgct gtactgggca acgacttcat gctttctttt ctttgtgata gccaatgtgt
     2761 cttcctcctt tcaaataaaa gtctttgcac cgctaggcag ggcaaagact cgcgaaagct
     2821 cgatctccat ttgaaaaccc gagattgtca ctaaaagcag gcaaaacttc tgtctacagt
     2881 gtcaagggtt ttcggctcgg tttctgaatt gcggtcttca ccttggcagg atattaagct
     2941 gtgccctggt tagggcaaga gcgcctgctg tctctggcga agcggtatta tcgataacgg
     3001 atcgcgttac tcgagcacct ccatggcttg cgcctggttc gggtccacct taatgccggg
     3061 acccatggtt gtggtgagcg tgatgcgctt gatgtaggta cccttagacg ccgctgggcg
     3121 ggctttcttt acagcctcca tcagcgccgc caggttctca tagagtttgt tctgctcgaa
     3181 agacactttt ccgatcggca catgcaggtt ggcggttttg tccaggcgga actcaacccg
     3241 accggcttta gattcgttca ccacccgcgg gatatcttca gcttggacaa cggtgccggc
     3301 cttggggttt ggcatcaagc cgcgcggacc caaaacccgg cccagacgac ctaccttccc
     3361 catcatatcg ggtgtggcaa tagccacatc aaaatcagtc cagcctgcct gaatcttggc
     3421 aagggtctca tcatcgtcgg cgaccaagtc tgcgccggcc tcccgtgcta gcgaggcgcc
     3481 ttcaccctgg gcgaaaacca ggactcgcac cgttttaccc aggccatggg gcagcaccac
     3541 cacgtcgcgc acttgctggt cggcatggcg aggatcaacg ccaagtcgca tgtggatttc
     3601 gacagtgccg tcgaacttgg atatagatgt ctccttggcg agagcaactg cttcggcagg
     3661 ggtgtagagt tgttccttat ctactttggc ggctgcagcc aggtatttct ttccgtgttt
     3721 cgtcatgaat cactccttcg tggtgcaagc gggcagtatg ctgccctccc accaggtcaa
     3781 tcgataatct gtatacccat attgcgggcg gtaccctcga tctggcgcat agcaccttcg
     3841 atatccaggg cgtttaggtc tttcattttt agctctgcaa tctcgcgaac ctgagcgcgg
     3901 gtgaccttgc ccactttttc gcgattgggc tgagcggatc ctttatcgac cccggcagct
     3961 tttctcaata aaacagctgc gggagaagtc ttgaggatga acgtgaacga cccatcggtg
     4021 tagatgctga tctctgcagg aatgatttcg cccatgcggc ttgaagtgcg ggcattatat
     4081 tccttgcaga atgccatcaa attgatcccg tgcccagcta gggctggacc gatggggggc
     4141 gcagggttgg ccttaccagc ctgtatctgc aatcggatga tagtcttaag tttctttgcc
     4201 aacgtacctt ctccttcgtg gtaaacgggt gatgggggaa caccctccca cctttatagc
     4261 cattcgccgc cagcctgaaa atgggtgctg atggtcgcag gctaattgct tctatgcctt
     4321 ttctacttgc agaaagtcca gctcaaccgg agtttcgcgg ccgaagaagt taaccatgac
     4381 acgcaccttg gctcgttcca tatcgatttc ggagaccgta ccacgaaaat cgttgaacgg
     4441 gccatccaca atacgcacac gctcgccgga tttaaatgta accttgatac gtggagcctc
     4501 ggcttccatg cggcggatga tttgcgcgac ttcctcgggg cggagtggcg tggggttgtt
     4561 gcccatgcca acaaatccgg tcaccccagg tgtgttgcgt acaacatacc aagattcttc
     4621 gctcaaaatc atgttgacca ggatgtagcc tgggaaaacc cgccgctcta ctgttcggcg
     4681 cttgccttcc ttgacctcga tctcttcttc agtggggaca acgatatcga aaatcttgtc
     4741 cttcatcccc atggtttcga tgcgctgctc caggttgtgg cgcaccttat tctcgtaacc
     4801 ggaatagcaa tgcactacat accaggcgcg gtcatcttcg gcctggtctt caatctcaac
     4861 cgggacgtcc aggctttctt ccgaatccaa caattcggcc gagagttcgt cttgcggcgt
     4921 ttcgacctca gtgatttcgt cttcggtcat caaaggttgc tcttccggaa ctttctcttc
     4981 cataaaaata ctactctctc gcctgccaat taatatttta tcagcttgct ttcaaacaat
     5041 ataattctac ccaaggatca gtgccattaa ggtcgtaaag atataatcca ggacgccaag
     5101 gtataatccc atcactacca tcacaaccaa aacgatcttg gtcagattcc aggcgtcctg
     5161 ccaggtgggc cagcttacct tgcgcaattc accaaccgtc tcacgaaaga aaagctggat
     5221 tttgttgctc gacctgcgct ctatagattt tcccttatca gctttggcca ttacggcctc
     5281 cttcatgggg tcacactact aaagataaag ccggtgaccc tcgccagtat tatttgttac
     5341 ggacaaaacg ccgccccggt agacggcgtt gcccttttta tggcaggcca ggcaggactt
     5401 gaacccacaa ccgctcgttt tggagacgag agctctacca attgagctac tggcctgttt
     5461 tatgcgctcc agagtcacgt cttatcttac catgtgaccc tggagcacct ggcactttac
     5521 ttcgtctcgc gatgtaaggt atgcttgcga cagcgcgggc agaatttatt taactccatg
     5581 cgacccggat cattgcgacg gtttttctcg gtggtgtaag ttcgttcctt acactccgtg
     5641 caagccagcg taattactgg gcggatatct ttcttagatg ccatagctta tctattaatc
     5701 cagaatctta gtaataacgc cggcgccgac ggtcaagcca ccttcacgga tggcgaactt
     5761 ggagccctgc tccagcgcca ccggcacgat caactccact tccaagttga cattgtcacc
     5821 cg
//
LOCUS       DBNHOH010000785         2648 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1331057, whole genome shotgun sequence.
ACCESSION   DBNHOH010000785 DBNHOH010000000
VERSION     DBNHOH010000785.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1331057"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="AC16V7_14100"
     CDS             complement(<1..545)
                     /locus_tag="AC16V7_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258301.1"
                     /translation="MLAALKSKMMHPLTNRLARPVDAVAHERLRLRLLFVVAILKLLN
                     FIGGAWDIQWHVAIGRDSLFIPPHLLVIFAFVAGLALVLALIIYETTMAATGQEMPHA
                     AKLGPFRAPPAFFGIFFGYTAALLSGVLDELWHEIFGIDATLWSPPHLLIMLATMVVD
                     FSLLLGIATSARRQGYEFKWS"
     gene            702..1823
                     /locus_tag="AC16V7_14105"
     CDS             702..1823
                     /locus_tag="AC16V7_14105"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR6258302.1"
                     /translation="MEFDLVFEGGGAKGLAFVGALQTFERRGHTPRRVIGTSAGSILA
                     VLIAAGYNAAECMAALNEKLPDGSSRFTSFLETPQIDEKIEINNSLRYWLKTELDNPM
                     IPNMIEPVVDQLVMGMVQKDLARHLISLLVWGGWYSGNALMGYLHEKLDAGGRNLGQS
                     TLLEFHHSTGRDLSVVASDVTGREMLVLNHRTAPDCPTTWAVRMSMSCPFVWHEVIWR
                     KEWGMYRGRDLSGHKVVDGGLLSNFPINLLVSTDDTIEEIMGRDSASEQVIGMLLDDS
                     MPVPGAEDTQKSESDAPSFLERMDLLAEMMLRIQGMAETVLGAHDKTIIDAHQHFVCQ
                     LPAKGYGILEFDLSAERMEPILKAGEATMEAHFQKYYDR"
     gene            complement(1879..>2648)
                     /locus_tag="AC16V7_14110"
     CDS             complement(1879..>2648)
                     /locus_tag="AC16V7_14110"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6258303.1"
                     /translation="GPALNTKSYLRNVTDEEIRAAIVRGLGESQMLAWGDLGLLTDRQ
                     IDELVEMIRAWEPTAPEVAIIAHQPPSAAQGDPGRGSELFAQFCSGCHGIEGETVLVD
                     GFVLSQLVSQLEDDVISKQINDGTEEMPSFHALLTRDDINDLLAFMRETFEGGGETSM
                     TSLPSFTQEILPLFNQKCVFCHGASGGWQSGSYDEVMNSGMNAPVVIPGDAEGSLLVQ
                     KLRGTQAEGAPMPPGTPLSETEIDLVVRWVEAGAPDN"
BASE COUNT          650 a          709 c          729 g          560 t
ORIGIN      
        1 ctgctccatt taaattcata gccctggcgc cgggcggagg tagcgattcc caacagcagg
       61 ctgaaatcaa ccaccattgt cgccagcata atcagcaggt gcggcgggct ccacaaggta
      121 gcatcgatgc cgaagatctc atgccataat tcgtctaata caccagacag caaggcagct
      181 gtatagccga agaaaatacc aaagaaggct gggggtgcac ggaaaggccc caatttggca
      241 gcgtgcggca tctcttgccc ggtagctgcc attgtggtct catagataat cagagccagg
      301 acaagcgcaa ggcctgcgac aaacgcgaag atgaccagca agtgtggcgg gataaacagg
      361 ctatcgcggc cgatagcgac atgccactgg atatcccagg cccctccaat aaagtttaac
      421 agcttcagga tggctacaac aaacaggagg cgcagacgaa gtcgctcgtg agcaaccgca
      481 tcaacagggc gagctagtct attggtgagg gggtgcatca ttttcgattt taaggcagca
      541 agcatcagaa tctcctcggt cggcggattc ggtagagcat tctaacaaaa ataatatttt
      601 ccatcaatag caaaatccag tataatgtat ctccttaggc gggatgcagg atcacattcc
      661 gtcccatccc ttaaaatcat gatatctagg aacagactaa gatggaattt gacttggtat
      721 ttgaaggcgg tggcgccaaa ggcctggcat ttgtcggtgc tttacaaacc tttgaacggc
      781 gagggcacac gccgcggcgc gtgatcggca catcggcagg gtcaatcctg gctgtgctga
      841 tagcagcggg atataacgcc gcggagtgca tggcagccct caacgaaaaa cttccagacg
      901 gcagttcgcg gtttaccagc ttcttggaga cgccccagat cgatgagaag atcgagatca
      961 acaacagctt gcgttactgg ctgaagacag agctggacaa cccaatgatc cccaacatga
     1021 tcgaaccggt agtggaccag ctggtgatgg gcatggtgca gaaggatctc gccagacact
     1081 tgatctcctt gctggtttgg ggaggttggt actctggcaa cgcgctgatg ggctacctgc
     1141 atgagaagct ggatgcaggc gggcgtaatc tgggccagtc cacactgctg gagtttcacc
     1201 attccacagg acgggactta tcggtggtcg cctcggatgt gaccggtcgt gagatgttgg
     1261 tgttgaacca ccgcacagcg ccagattgcc caacaacatg ggcggtgcgc atgtcgatga
     1321 gctgcccctt cgtctggcat gaggtgatct ggcgaaagga atgggggatg tatcgcgggc
     1381 gagacctgag cgggcacaag gtggtggacg gcggtctgct ctccaatttc ccgattaacc
     1441 tgctggtctc gaccgatgat accatcgaag aaatcatggg cagggattct gcctcggaac
     1501 aggtaattgg tatgctgctg gatgacagta tgccagtacc cggtgcagaa gatacccaga
     1561 aaagcgaatc cgatgccccg agcttcctgg agcggatgga cctgctggca gaaatgatgc
     1621 tgcgaattca gggtatggca gagacggtat taggcgcgca cgacaaaacc atcatcgatg
     1681 cccaccagca ctttgtgtgc cagctgccag ccaagggcta tggcattctc gaatttgatc
     1741 tctcagccga gcggatggag ccaatcctga aggccggaga ggcaacaatg gaagcgcact
     1801 tccaaaaata ctacgacagg tagcctgggg tcacccaaac ctatctttgt tttgttcttc
     1861 gccagaggct aaagccgctc agttatccgg cgcaccggct tcaacccagc gcactaccag
     1921 atcgatctct gtttcagaaa gaggcgtgcc aggcggcatg ggagcgcctt ccgcctgagt
     1981 gccgcgcagt ttctgaacca gaaggctgcc ctctgcatcg ccaggaatga ctactggtgc
     2041 attcataccg ctattcatca cttcgtcgta gctcccagat tgccaccctc cagacgcacc
     2101 gtgacaaaac acgcatttct ggttgaacag agggagtatc tcctgtgtaa aactaggcag
     2161 gcttgtcata ctagtttcac ccccgccttc gaaagtctcg cgcatgaacg ccagtagatc
     2221 gttgatatca tccctggtaa gcaaggcatg gaatgatggc atctcttcag tgccatcatt
     2281 aatttgcttg gagatcacgt catcttctaa ctggctgacc aactggctga gcacaaaccc
     2341 atctactaat acggtctccc cctcaatacc gtgacaaccc gagcaaaact gggcaaatag
     2401 ctccgagccg cgtcccgggt caccttgggc cgcgctgggg ggctggtggg caatgattgc
     2461 tacttctggg gctgtaggtt cccaggctcg tatcatttca acgagctcat cgatctggcg
     2521 atcagttagc aatccaagat caccccaagc cagcatctgc gattcgccca gcccgcgtac
     2581 gatggctgcg cgtatctctt cgtcagtgac attgcgcaaa taggacttcg tgttcagcgc
     2641 tggcccag
//
LOCUS       DBNHOH010000786         2446 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_176601, whole genome shotgun sequence.
ACCESSION   DBNHOH010000786 DBNHOH010000000
VERSION     DBNHOH010000786.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_176601"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1850
                     /locus_tag="AC16V7_14115"
     CDS             <1..1850
                     /locus_tag="AC16V7_14115"
                     /inference="COORDINATES: protein motif:HMM:NF044618.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258304.1"
                     /translation="KPTPSTPAIAASDPQYTLKILSDTLVPINDPADLAKRLEGKGDI
                     PPTLPPPSVPLQVGDRQSFWVLDTDTNENFRVDATLRYITDHAYFWIENNVDNNPRHL
                     RTLAETFENKIYPTNREFFGSEWSPGVDSDVHLYILYAGGLGSNLAGYFSSKDSYPPQ
                     IQEYSNGHEMFVFNADGTGLNDEYSFGVLAHEFQHMIHWYQDRNEETWLNEGFSDLAM
                     FLNGYSIGGHDFLYADDPDLQLNDWPNDSSQTAPHYGAAFLFVNYFLNRFGEQATQAL
                     VADPENGLESIDKVLSELGQIDPLTGGSVRADDVFMDWLLTSYIQDRRVGDGRYTYLD
                     YPRAPQPYEADEVRNCTNSSQKRQVSQYGADYILINCRGNFTLRFDGSKEVGVLPENP
                     YSGAYAFWSNKGDESDMTLTQEFDFTDHSGPLTLSYWTWYDIEKDFDYLYLEASLDGE
                     NWQILTTPSGTDQDLTGANFGWGYNNLSGGGPRWIFEEVDLSQFAGKKVQIRFEYITD
                     AAVNGEGFLLDDVSIPEIDYATDFETDDGGWTAEGFVRIQNTLPQEFRLALIKQGRAT
                     EVEYISLTADNTAEIPLQFGDGFNEAVLVVAGTTRFTRQSASYQFYFEP"
     gene            complement(1847..2074)
                     /locus_tag="AC16V7_14120"
     CDS             complement(1847..2074)
                     /locus_tag="AC16V7_14120"
                     /inference="COORDINATES: protein motif:HMM:NF024143.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HIR6258305.1"
                     /translation="MRRFMAFMMGAIMGGLVGATLALLLAPSSGTELRTQMQERVEGL
                     REEILAAAAERRTELEEQLAALRAPQKSGEA"
     gene            2189..>2446
                     /locus_tag="AC16V7_14125"
     CDS             2189..>2446
                     /locus_tag="AC16V7_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HIR6258306.1"
                     /translation="MASFIDPSLPWIDLHRHLDGSLRLETILDLGRKHNLPLPAWDVE
                     KMRPHVQITEPQPGVMAFIAKFKWMVGVLVDYAAVRRIAYEN"
BASE COUNT          570 a          705 c          620 g          551 t
ORIGIN      
        1 caaagccaac cccaagcaca cctgccatcg cggccagcga ccctcaatac acgctcaaga
       61 tcttgagcga taccctggta cccatcaacg atccggcaga tctggccaag cgcctggagg
      121 gcaaggggga tatcccaccc accctgccgc caccctccgt cccgctccag gtgggtgacc
      181 ggcaaagctt ctgggtcttg gatacagaca ccaacgagaa tttccgggtg gatgcgaccc
      241 tgcgctatat cacggatcac gcatactttt ggattgaaaa taatgtggac aataaccccc
      301 gtcatctgag aaccctggcg gaaacctttg aaaataaaat ttacccgacc aaccgcgagt
      361 ttttcggcag cgaatggtct cctggcgtgg atagcgatgt ccacctctac atcctttacg
      421 cgggcggctt ggggagcaac ctcgccggtt atttctcatc caaggactcc taccctcccc
      481 aaatccagga atattccaac ggtcacgaga tgttcgtctt caacgccgac ggcaccggtc
      541 tgaatgacga gtattccttt ggcgtgctag cccatgagtt ccagcacatg atccactggt
      601 accaggatcg caatgaagag acttggttga acgagggttt ttcggacttg gccatgttcc
      661 tcaacgggta cagcattggc ggtcatgatt ttctctacgc cgatgaccct gatctccagc
      721 taaatgattg gcctaacgat tccagccaaa ccgcccccca ttacggagca gctttcttgt
      781 ttgtgaatta cttcttaaac cgctttggcg agcaggccac ccaggctctg gtggctgacc
      841 cagaaaatgg cctggagagc atcgataagg tcttaagcga gctgggacag attgacccgc
      901 tcaccggggg gagcgtgcgc gccgacgatg tctttatgga ctggctgcta acgtcttaca
      961 tccaagatcg gcgtgtgggc gatgggcggt acacctatct ggattaccca agagccccgc
     1021 aaccctatga ggccgacgag gtgcgcaact gtacaaactc gtcgcaaaag cgccaggtga
     1081 gccagtatgg cgcggactat atacttataa actgccgtgg gaacttcact ttgcgcttcg
     1141 atggctcgaa agaagtgggc gtgctcccag aaaatcctta ttccggcgct tatgctttct
     1201 ggtcaaacaa aggcgacgag tcggatatga ccctgacaca ggagttcgac ttcaccgacc
     1261 atagcgggcc gctcacgtta agctattgga cctggtacga tatcgagaag gatttcgatt
     1321 atttatacct ggaggcttcc ctcgatgggg aaaactggca aattttgacc accccgtctg
     1381 gaacagacca ggacctcacc ggggccaatt ttggctgggg ttacaacaac ctcagcgggg
     1441 gcggtcccag gtggatattc gaggaagtgg atctctctca attcgcaggc aagaaagtgc
     1501 agatacgctt cgaatatatc accgatgccg cggtcaacgg cgaaggcttc ttacttgacg
     1561 atgtctcaat cccagagatt gactacgcta ccgattttga aaccgatgat gggggttgga
     1621 cggcagaagg tttcgtgcgt atccaaaaca cattgccgca ggaattccgc ctggcgctga
     1681 tcaaacaagg gcgcgctacc gaggtcgagt acatctctct caccgccgat aacaccgccg
     1741 agatccccct ccagtttgga gatggattta atgaagccgt gctggttgta gctgggacaa
     1801 cccgcttcac tcgccagtca gcttcctacc agttttattt cgagccttag gcttcgcctg
     1861 acttctgggg cgccctaaga gccgccagtt gctcctcgag ttcagttcgc cgttcggctg
     1921 ccgctgcaag aatctcctcg cgaagcccct ccacgcgttc ttgcatttgc gtgcggagtt
     1981 ctgtgccgga ggagggagct agcagtagtg ctagcgtcgc acctaccaac ccacccatga
     2041 ttgcccccat cataaaggcc ataaaccgcc tcatactgtc ctcctcaatt gtcaattttt
     2101 ctatttcagg atattataac aagaacttac cccgctatta catcaagtat cctgaaataa
     2161 agcatcacta accgacctag cctgggccat ggcttcattt atcgacccct ctctcccttg
     2221 gattgatcta caccgacacc tggatggaag cttgcgcctg gaaacgattc tggacctggg
     2281 gcgcaagcac aacctgcccc tgcccgcatg ggatgtcgaa aaaatgcgac cccacgtgca
     2341 aatcaccgaa ccgcagcccg gtgtgatggc ttttatcgcc aagttcaagt ggatggtcgg
     2401 ggtgctcgtc gattacgctg cagtgcgccg cattgcatat gaaaat
//
LOCUS       DBNHOH010000787         1307 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_754911, whole genome shotgun sequence.
ACCESSION   DBNHOH010000787 DBNHOH010000000
VERSION     DBNHOH010000787.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_754911"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..175
                     /locus_tag="AC16V7_14130"
     CDS             <1..175
                     /locus_tag="AC16V7_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
                     /protein_id="HIR6258307.1"
                     /translation="SSSVMINASTRFADGFEYGLGAEIGISTDKIHARGPVGLEGLTS
                     QKYVVLGDGHIRQ"
     gene            216..866
                     /gene="nadD"
                     /locus_tag="AC16V7_14135"
     CDS             216..866
                     /gene="nadD"
                     /locus_tag="AC16V7_14135"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HIR6258308.1"
                     /translation="MLGGTFDPIHHGHLRLAIELRDRLGFDQVRLLPCALPVHRESPG
                     CSALQRLEMVRLAVGQEPGLLVDDREVREDRPSYSYDTLQSLRAELGPHRSLTLVLGT
                     DAFLHLDSWHRWSELLQLCHILVVVRPGWHLPETHPMSRWLEQHRALEIEPLFYQSAG
                     SVLVQQLASLEISATAIREQVRSGKSPRFLIPDVVWSYIRQQGLYLSPASDCANQT"
     gene            879..1253
                     /gene="rsfS"
                     /locus_tag="AC16V7_14140"
     CDS             879..1253
                     /gene="rsfS"
                     /locus_tag="AC16V7_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIR6258309.1"
                     /translation="MQTEQLIEVVQTALGDIKARDVVLLDVRDKSSVTDYMIIASGSS
                     NRHVKSIADSVAVEAKKAGVTPLGIEGMETGEWALVDLVDVVVHVMQPHIRDLYDLEG
                     LWGLDAAPSTEDGKKTGEPTRG"
BASE COUNT          283 a          290 c          385 g          349 t
ORIGIN      
        1 ctccagttcg gtgatgatta acgcttctac acgctttgct gatggctttg agtatggcct
       61 aggtgctgag attggtatct ctactgataa aattcatgct cgtggccccg taggccttga
      121 aggcctgacc tcacaaaaat acgttgttct tggtgatggt catatccgtc aataatggct
      181 tgaaagaaca aagtgaaggt ccttcatcga tcgcgatgct gggtggtact tttgatccta
      241 ttcaccatgg ccatctccgg ttagcgatag agttgcgtga taggctgggt tttgatcagg
      301 tccgattgtt accgtgcgcg ttacctgtgc atcgtgaatc cccaggctgc agtgctctgc
      361 agcggctgga gatggtgcgc ttggcggtag gtcaggagcc agggctgctg gtcgatgatc
      421 gcgaagtccg tgaagatcgg ccctcttaca gttatgacac actgcaatca ttgcgcgccg
      481 agctggggcc tcacaggagt ttgaccctgg tgttgggcac agatgctttc ctgcatctgg
      541 atagctggca tcgctggtca gagttgttgc agttatgcca cattttagtg gtggtgcgtc
      601 caggctggca tctgccggag acgcatccga tgagccgttg gcttgagcag catcgtgctc
      661 tggagatcga accattgttc tatcagtctg ctggctctgt attggtacag cagctagctt
      721 cgctagaaat ttcagctact gcaattagag agcaggttcg cagtggaaaa tccccccgtt
      781 ttttaatacc cgacgttgtc tggagttata ttcgccagca gggtctttat ttgtcaccag
      841 caagtgactg tgcaaaccaa acctgagcaa gtaaatatat gcaaactgaa caattgattg
      901 aagtagtcca aaccgcactg ggcgatatta aggctcgaga tgtcgtgctg ttggatgttc
      961 gtgacaagag cagtgtgacc gattatatga tcatcgccag tggttcatcg aatcgtcatg
     1021 taaagtctat cgccgatagc gttgctgttg aggcgaagaa agccggtgtt actcccctag
     1081 gtatcgaggg gatggagacc ggagagtggg cactggtcga tctagttgat gtggtggtgc
     1141 atgtgatgca gccgcatatt cgcgatctct atgatcttga agggttgtgg gggctggatg
     1201 cggcgccaag taccgaggat ggtaagaaaa cgggtgagcc cacccggggc tagtccagat
     1261 gaagattcga ttgctctgcg tgggtactaa aatgcccggc tgggtcg
//
LOCUS       DBNHOH010000788         3421 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1780759, whole genome shotgun sequence.
ACCESSION   DBNHOH010000788 DBNHOH010000000
VERSION     DBNHOH010000788.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1780759"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="AC16V7_14145"
     CDS             <1..942
                     /locus_tag="AC16V7_14145"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chymotrypsin family serine protease"
                     /protein_id="HIR6258310.1"
                     /translation="HVVGVGTGYKISKGVRTSEMCIVTLVRQKMPVDGLSPKAIVSKQ
                     YDGILTDVIEVGDVRALQTPTSRWRPAPGGVSIGHYKITAGTLGAVVRDRASGDRLIL
                     SNNHVLANSNDAAVGDPILQPGPTDGGSTSSDVIAHLERFHPIDFSQEPGTCSLANSF
                     AELGNAIASAMGAKHRVQAIYANPQAANLVDGAVARPVSDNDIKDEILNIGVVSGTME
                     ATLGMSVRKSGRTTGFTTGEITVLDATVNVSYGTGRVARFDNQIVTGPMSQGGDSGSL
                     VVAGDALKAIGLLFAGSDQTTIINPIQAVLDSLSITI"
     gene            950..1213
                     /locus_tag="AC16V7_14150"
     CDS             950..1213
                     /locus_tag="AC16V7_14150"
                     /inference="COORDINATES: protein motif:HMM:NF051948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258311.1"
                     /translation="MQDLQAQTEKIRSVRRAYEKALMSKANVVGVGLGYRHHAGKRTD
                     ELALVVMVNKKVPLTQLAPEDVIPTKLEGVPVDVQEVGELKAF"
     gene            complement(1321..1794)
                     /gene="rpmE"
                     /locus_tag="AC16V7_14155"
     CDS             complement(1321..1794)
                     /gene="rpmE"
                     /locus_tag="AC16V7_14155"
                     /inference="COORDINATES: protein motif:HMM:NF015102.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HIR6258312.1"
                     /translation="MKSEIHPVFYPDAQVICACGNTWTTGSTKKVIHTDVCSNCHPFY
                     TGEQRIVDTEGQVDRFYRKLQARQQYLSEVEAREAARISPERPITELDLGPRVTDKLA
                     KAGIVNVGQALDKLALGEASLLSIDGFGRKSLADLKKRLRSFGYSIPEEGEEITV"
     gene            complement(1845..2108)
                     /gene="rpmA"
                     /locus_tag="AC16V7_14160"
     CDS             complement(1845..2108)
                     /gene="rpmA"
                     /locus_tag="AC16V7_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012626133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR6258313.1"
                     /translation="MAHKKGGGSSRNGRDSQSQRLGVKVFGGQFVSSGSILVRQRGTR
                     IKPGRNVDVGRDDTLFATSDGIVVYETIRDDRKRVSVIPETTA"
     gene            complement(2119..2436)
                     /gene="rplU"
                     /locus_tag="AC16V7_14165"
     CDS             complement(2119..2436)
                     /gene="rplU"
                     /locus_tag="AC16V7_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIR6258314.1"
                     /translation="MKYAIVEDGGKQYKAVEGATIDVDRFVADEGDQLDMERVLLVVD
                     GDDVKVGTPLVAGAKVQATVISQVKGPKVVVFKYRPKQRYRVKKGHRQQYTRILIDSI
                     TLE"
BASE COUNT          879 a          869 c          857 g          816 t
ORIGIN      
        1 catgttgttg gagttggaac ggggtataag atatccaaag gggtgcgcac cagcgagatg
       61 tgcattgtta cattggtccg ccagaaaatg ccggtggatg gcctatcacc aaaagcgatt
      121 gtatccaaac agtatgatgg aatattgacc gatgtgatcg aagtgggtga tgtgagggcg
      181 ctacaaaccc cgacttcccg ctggcgacct gccccaggtg gggtgagcat cggccactat
      241 aaaattactg ctgggacgct aggggctgta gttcgcgacc gggcaagcgg agatcgcctg
      301 atcttatcca acaaccatgt cctggcgaat agcaacgacg ccgctgtcgg cgatcccatc
      361 cttcagcctg gtcctacgga tggaggcagc acttcttcgg atgttatcgc tcacctggag
      421 cgctttcatc caatcgattt cagccaggaa ccaggcactt gcagcctggc taattctttt
      481 gccgagttag gcaatgccat cgccagcgcg atgggagcca aacaccgcgt gcaagctatc
      541 tatgctaacc cgcaggccgc taatctagtg gacggcgccg tcgcccgccc ggtgagcgat
      601 aacgatatta aagatgaaat cctgaatatt ggtgtggtct ccggcaccat ggaagcaacc
      661 ctgggcatgt cagtgcgtaa atccgggcgc accaccggct tcaccacagg tgagattacc
      721 gtcttagatg ccaccgtaaa cgtaagttat ggcacaggac gggtagcccg cttcgacaac
      781 cagattgtca caggacctat gtcgcaaggc ggcgattcgg gatccttggt cgttgctgga
      841 gatgccttaa aagctatcgg tttgctcttc gccggatcgg atcaaaccac catcatcaac
      901 cctattcagg cggtgctgga tagcctaagt atcacaattt gagtttatta tgcaagattt
      961 acaagcccaa actgaaaaaa tccgctcagt ccgccgcgca tatgaaaaag ccctgatgtc
     1021 caaagccaat gtagtcggcg tgggtttagg ctatcgccat catgctggca agcgaacgga
     1081 tgagctggcg ttggtggtca tggtcaataa aaaagtaccc ttaacccagc tcgcccccga
     1141 ggatgtcatc cccaccaaat tggaaggggt tccggttgat gttcaagagg tgggagaact
     1201 gaaggctttt taataaacag acctggtgct gtatgagcag tcaattgttc atcacagaac
     1261 accaggtctt ggttccgtat tgtatgcccc cggataggat cggctagggg ctaagtgtaa
     1321 ttaaacagtg atttcctcgc cttcctcagg gatggagtaa ccaaacgacc gtaatcgttt
     1381 ctttaggtct gccagcgatt tgcgaccgaa accatcgatg ctgagcaagg aagcctcccc
     1441 taaagccaac ttatccagcg cctggccaac gttgacaata cctgccttgg ccagtttgtc
     1501 ggtgacacgc ggtcctaggt caagctctgt aataggccgc tctggggata tgcgagctgc
     1561 ttcgcgagct tcgacctcgc ttaaatattg ctgtcgggcc tgtagcttcc tatagaagcg
     1621 atcgacctga ccttcggtat ccacgatgcg ctgctcacca gtgtagaacg gatgacaatt
     1681 tgagcaaaca tcagtatgga tgactttctt agtcgagccg gttgtccaag tatttccgca
     1741 cgcgcagatc acctgggcat ccgggtaaaa aacgggatgg atttcggatt tcataagtta
     1801 accctttaac tttaatagcc ttctttccac atatgtggaa gacatcaagc agtagtctcg
     1861 ggaatcacgc taacgcgctt gcggtcatcg cgtatagttt cgtagaccac aatgccatcg
     1921 ctcgtagcga acagagtgtc gtctctaccg acgtctacat tgcgacctgg tttaatccgc
     1981 gtgccgcgct ggcgcactag gatgcttcca gaacttacaa attgcccgcc aaagacctta
     2041 acgcccaggc gttgagactg actatctctt ccgttgcggc tggagccgcc acctttcttg
     2101 tgagccatag ttacctcgct attccagcgt aatggagtcg atcaaaatcc gggtatattg
     2161 ctggcgatgc ccctttttga cgcgataacg ttgcttgggc ctgtacttaa aaaccacaac
     2221 cttcggacct ttcacctgag agataactgt ggcctgtact ttggcacctg caacgagagg
     2281 agtgccaact ttcacatcat cgccatcgac caccagcaag acgcgttcca tgtctagctg
     2341 gtccccttca tcagccacaa agcggtcgac atcgattgtg gccccttcga cggctttata
     2401 ctgcttaccg ccatcttcaa caatggcata tttcatttga aattcctcca agcttcactt
     2461 tgccatagtc cacgcgctcc gatcgcaacc agcatccagc aagaaggatg cgcccaggta
     2521 cgggaaagtt gggggtacaa gatatagccc cagcagcgtg cagccggggc agtgcggata
     2581 gggattatac ctgagaggcg gctggttgtc aacagatgcg agaaataaaa ttaagtatct
     2641 aagcaatcac aatatcagac tttgcacaca atagtgagtt aaattcccgc cgagagacta
     2701 ggaataatac gctcaagaaa gtatccccct taaagtatct acctacattc cttataattt
     2761 catcagattg tgtttattcc agcctgaacc tgtaacagat gcagccaaaa accgtttttt
     2821 ctagtagatc ttgaaaaata ggttgaagga gcactggatt tgaacaacga gctactaccc
     2881 cacgtcatgg ttttggagaa catacactgc gcggatactg aagaatggta ttgtcccacc
     2941 tgtggtcgac gaatattgat gaagtggcca ccgaattata aaaaagttat tttggatgca
     3001 ggcgatgaag aagtcatcca ttgcgtaggc agaggtggtt tgccctttgg aaccccgcaa
     3061 gtctcccaga atcgagaaac attatctgag gacattcaac ggttgatcca gtgggatgtc
     3121 tggctagagc aaataggttt cgaaagctgg tgggatcggt aatcaaaagg tttgtctata
     3181 cgcaatatcc acagggggac aagcaacata cattcggggt gatccagaca aaagatcaat
     3241 cccagggtgc agagtgtttc caggcagtaa ccgccggcat ttagggggct cgctattaat
     3301 gctaattcca gcgctgaatg caagagaaat tgaaccctcc tcaagaccga agttcaatcg
     3361 ctcgtagctt cacaactact tcgcaacaaa ggtgcataca gtttgtacct ggtgacgacc
     3421 a
//
LOCUS       DBNHOH010000789         3661 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_883388, whole genome shotgun sequence.
ACCESSION   DBNHOH010000789 DBNHOH010000000
VERSION     DBNHOH010000789.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_883388"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="AC16V7_14170"
     CDS             <1..886
                     /locus_tag="AC16V7_14170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HIR6258315.1"
                     /translation="GYGRLNPFTIPAGIPNLPAFLVAQRFQCLGRNSTIVTACATGTQ
                     AVGEGSEYIRRGVSDIVIVGGTEALIRDFSIGGFSSMRALPTNFNANPESASRPFDAK
                     REGFIFSEGAGAIVLESLEHAQARGAKIYAELLGHSSSSDGYHMASPDPYALGPARAM
                     RWALEDANLEPEQVDYINAHGTSTPLNDPTETLAIKIVFGDHAHRLSISSTKSMLGHA
                     MGASGTLEAIICALSIKKGIIPPTINYEYPDPACDLDYVPNQARKQQVKIAMSNSFGL
                     GGQNACLVMSSFSTNGVK"
     gene            883..1587
                     /locus_tag="AC16V7_14175"
     CDS             883..1587
                     /locus_tag="AC16V7_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258316.1"
                     /translation="MKIYTNEKLVARNRRIAQIASLSGLVVLVGGMIISFQRQDLINY
                     SFAALILGFILSQIGIYFTTRWGRSPRPDELINQALKGLDDKYSMYHYATPASHFLVG
                     PAGLWVLMPRHQRGTISLYKDRWRHTGGGFMQTYLKLFGQESLGRPELEMQNEIEKLK
                     KKLQKMMIEGGLPDIQAALIFTSDDIDIQIGDDETPPAPTLYINKLKEFIRKTAKTKP
                     VSLSKIEEIQQVLSSE"
     gene            complement(1625..1855)
                     /locus_tag="AC16V7_14180"
     CDS             complement(1625..1855)
                     /locus_tag="AC16V7_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258317.1"
                     /translation="MSHEESAKNVPWFLWPVWALWRLIALIVGFTGRLIAVVLGFVLI
                     VVGIVVSVTVVGMIIGIPMILIGVLLVIRGLF"
     gene            complement(1991..2473)
                     /locus_tag="AC16V7_14185"
     CDS             complement(1991..2473)
                     /locus_tag="AC16V7_14185"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6258318.1"
                     /translation="MDPSRYTLIPRVLIFLMCGERVLLLKGAPTKRIWPNLYNGIGGH
                     VERGEDVLSAARRELLEETGMVVSDLWLCGVVSVDTGQNPGIGIFVMRGECPEEVLAA
                     SGEGQPEWVDILEIQKLPLVEDLFTLLPRVFQARRNDPPFFASYRYDQNDKLVISIGN
                     "
     gene            complement(2502..3458)
                     /locus_tag="AC16V7_14190"
     CDS             complement(2502..3458)
                     /locus_tag="AC16V7_14190"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0030388 - fructose 1,6-bisphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HIR6258319.1"
                     /translation="MIVTTKKLFELAYGKYAIGAYNINNLEQCMGLFRGNLDSQAPFI
                     IQISKGARSYTHKKMLEGLIRAAEDVFPDSIFAVHLDHGDEEACYDCIDSGFYSSVMI
                     DASHEDFENNIAITRRVVEAAHAKGVVVEAELGQLGGVEEHVKVDEADAKLTNPDQAE
                     EFVERSGCDSLAVAIGTSHGAYKFSGSQALRLDVLEKIQQRLPGFPLVMHGSSSVPQE
                     EVERINKAGGQLKGAKGVDADQFKYAAKLGVTKVNIDTDGRLVWTRVHREYFNEHPEN
                     FDLRPPGKVFMGEYAKFIAGKNEKLGSAGQLETVRKLVRQKA"
BASE COUNT          987 a         1010 c          775 g          889 t
ORIGIN      
        1 ggggtacggg cgtctcaacc ccttcacaat tcccgccggt atccccaacc tgcccgcttt
       61 cctggtagcc caaaggttcc aatgcctcgg acgcaacagc accatcgtta ctgcttgcgc
      121 aaccggcact caagcagttg gagaaggttc cgaatacatc cgccgcggcg taagcgacat
      181 cgtcatcgtc ggtggcaccg aggcattgat ccgtgatttc tccatcggtg gattctcgtc
      241 catgcgtgcc cttcctacta atttcaatgc caaccccgaa tctgcctcgc gcccttttga
      301 cgccaaaaga gagggattta tattttccga gggtgccgga gcgatagtac ttgagagtct
      361 ggaacatgca caggcacgcg gggcgaaaat atacgctgaa ttgcttggtc actcttcgtc
      421 cagcgatggc tatcacatgg cttcgccaga tccttatgct cttggaccgg cacgagctat
      481 gcgttgggca ctcgaagacg caaatctcga accggagcag gtagattaca tcaacgctca
      541 cggtacatcc acccccctta acgatccaac cgaaacgcta gcaatcaaga tagtttttgg
      601 cgatcatgcc catcgcctgt caattagctc caccaaatcc atgcttggtc acgcaatggg
      661 cgcttcaggt accctggagg ctatcatttg cgctcttagc atcaagaagg gtattatccc
      721 gccaactatc aactatgaat accctgaccc ggcgtgcgac ctggattacg tgccaaatca
      781 ggcgcgcaaa cagcaagtaa agattgcaat gtcaaattcc tttggactgg gaggccagaa
      841 cgcctgccta gtaatgagta gtttttcaac taacggagtg aaatgaaaat atatacgaat
      901 gaaaagctgg ttgcgcgcaa ccggcgcata gcacaaattg ctagcctcag cggcctggtg
      961 gtgttggtgg gagggatgat catatcattc cagagacaag atctgatcaa ctattccttc
     1021 gcagcactca tcttgggatt tattctctcc caaattggta tttatttcac cacacgttgg
     1081 gggcgtagcc cacgaccaga cgaattaatc aaccaggcgc tgaaggggct ggacgacaag
     1141 tattccatgt accactacgc cacgccagcc tcccactttt tagtgggtcc tgccggactt
     1201 tgggtgttga tgccccgtca ccagcgcggc acgatcagcc tgtataaaga tcgctggcgc
     1261 cacactggag ggggattcat gcaaacctat ctaaagctat ttgggcagga gagcctggga
     1321 cgccctgagc tggagatgca gaatgaaatc gagaaattga aaaagaagct acaaaaaatg
     1381 atgattgagg gcggtcttcc tgatatccag gccgctttga tcttcacaag tgatgatatt
     1441 gacatacaaa ttggtgatga tgaaactccc ccagcaccga ctctctatat taataagctg
     1501 aaagaattca tccgcaaaac agcaaaaact aaacctgtct cactatccaa aatagaagaa
     1561 attcagcaag tcttgagctc cgaataacca aaaagcgggc aagaaacgcc cgctttttgg
     1621 ttatttaaaa caggcctcga atgaccagca gcacgccaat aaggatcatt ggaataccta
     1681 tgatcatacc cacaaccgtc acacttacaa ctattccaac tacaatcaac acaaagccca
     1741 atacaacggc aatcaagcgt cccgtaaagc caacaatcaa ggcaattagg cgccagagag
     1801 cccagacagg ccacagaaac cagggcacat tttttgcaga ctcttcgtga gacatatcct
     1861 tcactcctca atattagtag attcattatt tattacgtaa cccccaataa aaggtttcaa
     1921 tccaatctgc cacttttgcc cttgtaagga tcccccatcg taaagctaac agcaattctt
     1981 aaaatgaaat ctaattacct atgctaatta ccaatttatc attctgatca tagcgatagc
     2041 ttgcaaagaa tggcggatca tttcgtctcg cctgaaatac tctgggtagt aatgtaaata
     2101 gatcctcaac aaggggcagc ttttgaattt ccaatatatc cacccactcc ggctgccctt
     2161 caccagatgc agctagcacc tcttcaggac actccccccg catcacaaag atgccaatac
     2221 ccgggttttg gccggtatcc acagaaacca caccacataa ccacaaatcg gagactacca
     2281 ttccagtctc ctcaagcagc tctcttcggg cagcactgag cacatcttcg ccacgctcta
     2341 cgtgccctcc gataccattg tataggtttg gccagattcg cttggtcggc gcgcctttca
     2401 ataagagtac gcgctcaccg cacatcagga atataagtac gcgcgggatc agcgtataac
     2461 gactaggatc cacaccttga tccacagccg gcatgatctg tttaggcttt ctgtcgtact
     2521 aatttccgaa cggtctcaag ctggccggcg ctgccaagct tttcattctt tccggcaatg
     2581 aacttagcat attcgcccat aaaaaccttg ccaggcgggc gcaaatcgaa gttctccggg
     2641 tgttcattga aatattcgcg gtgaacccgc gtccacacca agcgcccatc ggtatcgatg
     2701 ttcaccttgg ttacacccag cttggcggcg tacttgaatt gatccgcatc cacacctttg
     2761 gcgcctttga gctgtccgcc ggctttgtta atgcgctcaa cttcttcctg tggcaccgag
     2821 ctactgccat gcatcaccag cggaaaacca ggcagccttt gctgaatctt ttctagcaca
     2881 tctaaacgta aagcttggga gccactaaat ttatacgccc catgactcgt tccaattgca
     2941 actgccaggg aatcgcatcc agatctctcc acaaactcct ctgcctgatc tgggttcgtg
     3001 agcttagcat ctgcctcatc caccttcaca tgctcttcta caccacccaa ctgacccagc
     3061 tcagcctcta ccaccacccc cttggcatgg gctgcctcaa ccacacgccg tgtaattgcg
     3121 atgttatttt cgaaatcctc atggcttgcg tcgatcatca ccgagctata gaatccacta
     3181 tcaatgcaat cgtaacatgc ctcttcgtca ccatggtcca ggtgtacagc aaaaattgaa
     3241 tctgggaaaa cgtcttctgc agcccgtatt agcccttcca gcatcttttt atgggtgtaa
     3301 ctgcgtgcac ctttgctgat ctggataata aatggcgcct ggctatcaag attgccacga
     3361 aacaacccca tacactgttc taggttattg atgttatagg ctccgatggc gtatttacca
     3421 taagcaagct caaagagctt ctttgttgtg acgatcatga tatactcctt atatattgcg
     3481 aaagtgacgc agctattata aaccatccga gcaacttttc atggcacagt acgtatattt
     3541 atcgaaggat catcgttgcc tcgaattaaa actgaggcta taatcaatcc atacagactg
     3601 ttgcgaaaac acaccaacag aaggagaatg aaatgagtga tttgatcgat cagatcatgg
     3661 g
//
LOCUS       DBNHOH010000790         4252 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_627271, whole genome shotgun sequence.
ACCESSION   DBNHOH010000790 DBNHOH010000000
VERSION     DBNHOH010000790.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_627271"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="AC16V7_14195"
     CDS             <1..213
                     /locus_tag="AC16V7_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258320.1"
                     /translation="LSSLMSPCFPWGDIFPEQSGGVNIAEQQHPIGGDLDIHSTRDKL
                     MTPMDGKNTLPTPTPAPRKPERAFVH"
     gene            331..2097
                     /locus_tag="AC16V7_14200"
     CDS             331..2097
                     /locus_tag="AC16V7_14200"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018743953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR6258321.1"
                     /translation="MSQLFSQTLREAPSEAEIPSHQLLLRAGFIRQLAAGIFSYLPLA
                     KRALTKIEDIMRDEMDAIGGQEVTMPVVHPAEVWQETERWYQIGSEMGRFKDKNNRDM
                     VLAMTHEEVVADLVRKEIQSYRQLPRLIYHIQTKWRDDPRPRAGLIRVREFTMKDSYS
                     LDADWDGLDQQYRAHYQAYFNIFHRCGVPAIAVKSDVGMMGGKLAHEYMYITPIGEDT
                     LLLCNSCGYAANRQIARFKKAPSASEAPQPVEKVATPNTTTIEGLANLLQVTKAKTAK
                     AVFLVASIPEGREVVERFVFAVVRGDMEVNETKLANALGAQSLRPATEDEITAVGAVA
                     GYASPVGLKNALVVVDDAIPSSPNLVAGANEDGYHLLNVNYERDYTANLVTDIAAAQD
                     GDACPNCGGELHTVRGVEVGNIFKLGTRYSDSMGCKYLDRDGVEKPVIMGSYGIGSGR
                     LLACVAEEHHDDFGLIWPITVAPYHVHLVVLGSKEGSDTRLAADQLYGDLLAEHIEVL
                     YDDRDESPGVKFNDADLIGLPIRLTISERTLKQGNVEFKRRDSKEKELIPRSDVITHV
                     KNEIQALKAAIQAQVVQVSFDE"
     gene            complement(2128..3069)
                     /gene="metA"
                     /locus_tag="AC16V7_14205"
     CDS             complement(2128..3069)
                     /gene="metA"
                     /locus_tag="AC16V7_14205"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015945319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase MetA"
                     /protein_id="HIR6258322.1"
                     /translation="MPVKIPETLPARQILESENVFVMGETRARHQDIRPIKLAILNLM
                     PTKIATETQLLRLLGNTALQIDITLLHMDSHHSKNTPSDHLLAHYRSFRQVQGEKFDG
                     MVITGAPVEHLPFEQVDYWDELKTIMEWTETHVYSTMHICWGAQAGLYSRFGVPKHPL
                     PAKMFGVFPHRVNFRNELLLRGFDDEFYAPHSRHTEIRKEDIQPVGDLDILSESDEAG
                     VYIVASRDRRQFYITGHSEYDPLTLKAEYDRDQGNGMPITIPKNYYPHDDPSQLPIVR
                     WRGHANLLFANWLNYYVYQITPYDRKKIPQGGTYATF"
     gene            complement(3080..>4252)
                     /locus_tag="AC16V7_14210"
     CDS             complement(3080..>4252)
                     /locus_tag="AC16V7_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HIR6258323.1"
                     /translation="YVFNDTDHAARLFALEEVGNIYTRIMNPTNDVFEQRLADLEGGV
                     GALAASSGHAAQSMAILTLCGAGDHIVSASTLYGGTYNQFCYTFPRLGIDVTFVDPSD
                     PENFRRAIRPNTKLLWGETLGNPRINIFPIQEVAAIGQEFGIPLAIDNTFATPYLCRP
                     FEWGANIVVHSATKFIGGHGTSIGGIIVDGGYFEWAKNERFSNFNIPDDSYHGLVYAD
                     LGPAAFITKARVQILRDIGACQSPFNSWLFLQGLETLSLRMERHVSNAQSVAEFLQDH
                     PHVSWVSYPGLPSHPDYHAAQKYLPKGPGAILGFGVRGGLEAGRRFIDRLQLFSHLAN
                     VGDAKSLAIHPASTTHSQLTTEQQLSAGVSPDFLRLSVGIEDIDDILFDLDQALSS"
BASE COUNT         1058 a         1128 c         1126 g          940 t
ORIGIN      
        1 ctttcatcgt taatgtcacc ttgttttcca tggggtgaca ttttccctga acagtcaggg
       61 ggtgtcaata tcgctgaaca acaacacccg attggaggcg accttgacat tcattcaacc
      121 cgtgataaac taatgacacc catggacggg aaaaatactc tacctacccc aacccctgcg
      181 ccgcgcaagc ccgagcgggc tttcgtccat tagggattca gtggtagtta catagacgcc
      241 ctcggacgga cagcccgggg gttttattat gcactgagaa tactaaatac atgacactgg
      301 aaacctgaca caggagcacg tccaatgaga atgtcccaac tcttcagcca aactttacgt
      361 gaggcgccat ctgaagccga aattccaagc caccagctct tgctccgggc aggctttatc
      421 cgccagctcg ctgcgggtat cttcagctac ctgccgctgg ccaagcgcgc cctgaccaag
      481 atcgaggaca ttatgcgaga cgagatggat gccattggcg ggcaggaggt gaccatgccc
      541 gtggtacacc ccgctgaggt ttggcaggaa actgagcgct ggtaccaaat tggctctgag
      601 atggggcgct tcaaagacaa gaacaaccgc gatatggtgc ttgccatgac ccacgaagaa
      661 gtggtcgccg accttgtgcg caaagaaatc caatcctatc gccagttgcc gcgcctgatc
      721 taccacatcc aaaccaaatg gcgcgatgac ccccgcccgc gcgccgggct gatccgggtg
      781 cgcgaattca ctatgaagga tagttacagc ctggatgccg actgggatgg cttagaccag
      841 cagtaccgcg cccattacca ggcatacttt aatatttttc accgttgcgg cgtgccggct
      901 atcgcggtga agtccgacgt gggcatgatg ggcgggaagc tggcccatga atatatgtac
      961 atcaccccaa ttggtgagga taccctcctg ctgtgcaatt cttgcggtta tgctgccaac
     1021 cggcaaattg cccgctttaa gaaggcgcct tccgcatcag aagcacccca gccggtggag
     1081 aaagtcgcaa cacccaacac gaccactatt gaggggctgg caaacctcct gcaggtgacc
     1141 aaggcgaaaa ccgccaaagc cgtattcctg gtagcctcca tccctgaagg gagagaagtg
     1201 gtcgagcgct ttgtctttgc tgtggtgcgt ggggatatgg aggtgaacga gaccaaactg
     1261 gcgaacgcat taggagccca atccttgcgc cctgccacgg aagacgagat cactgccgtc
     1321 ggcgcggttg ctggctacgc atcaccggtt gggttgaaaa atgccctggt ggttgtggat
     1381 gatgccatcc ctagctcacc caacctggtc gcgggcgcca atgaagacgg ctaccacttg
     1441 ctcaacgtga attacgagcg cgactacact gccaatctgg tcaccgatat cgcagccgcc
     1501 caggatggag atgcctgtcc caattgcggc ggtgagctgc ataccgtgcg cggcgtggag
     1561 gtaggcaata tcttcaagct gggcacacgt tacagtgatt ctatgggatg caaatatcta
     1621 gacagggacg gcgtggaaaa accggtcata atgggctcct acggtatcgg cagcgggcgc
     1681 ctgctggcat gcgtcgccga ggagcatcac gacgactttg ggctgatctg gcctattaca
     1741 gtggccccct atcatgtcca tctagttgtt ctgggtagta aggaaggctc agatacacgg
     1801 cttgccgctg accagcttta tggcgatttg ctagcagaac atattgaagt cctgtatgat
     1861 gacagggatg agagccccgg ggtgaaattt aatgacgctg atctgatagg cttgcccatc
     1921 cgccttacaa taagcgagcg caccctgaag caaggcaacg tggagttcaa gcgccgcgac
     1981 agcaaagaaa aagagcttat cccgcgcagc gatgtcatca cccatgtcaa gaatgagatc
     2041 caagccttga aagcagccat ccaggcacaa gtagttcaag tgtcttttga cgagtaattc
     2101 gggctgctct aatttcacat ctctcattta gaaggttgca tacgtacctc cctgagggat
     2161 ctttttccta tcatagggtg tgatctggta cacataataa tttaaccaat tggcaaacaa
     2221 caggttggca tgcccgcgcc atcgcacgat gggtagctga gaaggatcgt catgagggta
     2281 gtaattcttc gggatggtaa tcggcatccc gttaccctgg tcgcgatcat attcagcctt
     2341 caaggtcagt ggatcgtact ccgaatgacc agtgatatag aactggcggc gatcccggga
     2401 agccacaatg taaaccccag cttcatccga ttcagatagg atatccaaat cgccaaccgg
     2461 ttggatatct tctttacgaa tttcggtgtg ccgagaatgg ggggcataaa actcgtcgtc
     2521 gaagccgcgc aatagcaatt catttctaaa attcactcga tggggaaata caccaaacat
     2581 tttagctggc agcgggtgtt tggggacgcc aaaccggcta tacagtcccg cctgtgcacc
     2641 ccagcatata tgcattgtgg aatagacgtg cgtctcggtc cattccataa tagttttcaa
     2701 ctcgtcccaa tagtccactt gttcaaaagg caaatgctca accggcgcgc cggtgatcac
     2761 cattccatca aacttttcgc cctgaacctg gcgaaaagag cgataatgag ccagcaaatg
     2821 atctgagggt gtattcttcg aatgatgaga gtccatatgc agcagggtaa tatcaatctg
     2881 cagcgcggtg ttccccagca atctcaagag ctgtgtctcg gtagcgattt tcgtcggcat
     2941 caagttcagg atagccagtt tgataggtct tatatcttga tgccgggcgc gtgtctcacc
     3001 catcacgaag acattttcgc tttcaagtat ttgcctggca ggcaaggttt cagggatttt
     3061 aaccggcatg tgagcctcct caggatgata atgcttgatc aaggtcgaac aggatatcgt
     3121 caatatcttc gatgccaaca cttaaccgca aaaagtcggg gctgacaccc gcgctgagct
     3181 gctgctcagt agtgagttgg ctgtgcgtag tggaagcagg gtgaatcgcc aaactttttg
     3241 catcgcctac attggccaaa tggctgaaaa gttgcaatcg gtcgataaac ctgcgccctg
     3301 cctctaatcc ccctcgcacc ccaaaaccaa ggatcgcccc tggtcccttc ggcaagtatt
     3361 tctgggcggc atggtaatct ggatgagaag ggagaccagg gtacgacacc cagctaacat
     3421 ggggatgatc ctgcaggaat tcagccaccg actgggcatt gctcacatgg cgttccatcc
     3481 gcaggcttag ggtttccaag ccttgcaaga acagccaaga gttgaagggt gactggcaag
     3541 caccaatatc gcgtaaaatc tggacacgcg ccttggtaat gaacgccgcc gggcctagat
     3601 cagcatacac aagcccatga tagctatcat cgggtatatt gaaatttgaa aatctttcat
     3661 tctttgccca ttcaaaatat ccaccatcta caataatgcc gccaatggat gtgccatgac
     3721 cgccgataaa tttggtcgcg gaatggacga cgatattggc tccccattca aaaggccggc
     3781 acaggtatgg cgtagcaaag gtgttatcaa tcgccagcgg tatgccgaat tcctgcccta
     3841 tggcagccac ctcttggata gggaagatgt tgatgcgggg attgcccagc gtctcgcccc
     3901 acagcaattt ggtgttagga cggatggcgc ggcgaaaatt ttcggggtcg gaaggatcta
     3961 caaaggtcac gtcaatgccg aggcggggaa aggtgtagca aaactggtta taggtgccgc
     4021 cgtaaagtgt cgaggcagac acaatgtgat ccccagcacc gcacagtgtc aaaatcgcca
     4081 tgctttgcgc agcatgaccc gaacttgcag ccagtgcacc cacaccccct tccaggtctg
     4141 ccaagcgctg ctcaaacaca tcgttcgtgg ggttcatgat acgggtatag atgttcccca
     4201 cctcctccaa agcgaagagg cgggcagcat ggtcggtgtc gttgaaaaca ta
//
LOCUS       DBNHOH010000791         1880 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_818475, whole genome shotgun sequence.
ACCESSION   DBNHOH010000791 DBNHOH010000000
VERSION     DBNHOH010000791.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_818475"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="AC16V7_14215"
     CDS             complement(<1..1002)
                     /locus_tag="AC16V7_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HIR6258324.1"
                     /translation="MSIERLLEKPGRMFRVIFDRLSLPVQMILGFVFVVVLTAVTIGV
                     PTIWLLRHDLDRQAWIVVEQGVRAADAFCAAQQKEIIDFATLTAERPTLHEFLSQDNR
                     RDLLDYLVTLKNAAAYDLIVVCDVDDNVVTATENMVSNQICLEKDIGGMYLVDSQEPP
                     QALMVAIHPIVMDSHTIGRVIVANRIDHAFATQMRAFSGLENTLLVSGRPVASSFDFD
                     LSDLSNIPHQLIAAGSPSGIEQSQFKLDGQPYYAEKVPLEYLGVTVEVALLVSDIVRS
                     QQRLLWLLIGGMLGVASLVSALGVYISQQISKPLVDLAETAAEFSMGELGSPVSIEAR
                     "
     gene            complement(1014..1271)
                     /locus_tag="AC16V7_14220"
     CDS             complement(1014..1271)
                     /locus_tag="AC16V7_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor-like protein"
                     /protein_id="HIR6258325.1"
                     /translation="MEVRVGKVTHFYNRISVAVLELSGNLEIGDKILILGRTSELTQV
                     VTSMEIEHRKVQTVGPGMDVALKVAEPVRKGDIVYKIIDNE"
     gene            complement(1299..1661)
                     /locus_tag="AC16V7_14225"
     CDS             complement(1299..1661)
                     /locus_tag="AC16V7_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HIR6258326.1"
                     /translation="MNPQIDSTNFGSITITGTKFQHDVIIRLDGKIEKRKKKLSKAIY
                     GTSHIISLDEARHIYEDGMEQLVIGAGQFGRVKLSEEAENFFREKAIRVAIMPTPRAV
                     EAWNSAKTGVIGLFHVTC"
BASE COUNT          505 a          509 c          385 g          481 t
ORIGIN      
        1 acgcgcctca atcgatacag gcgaacccag ttctcccatg ctgaactcag ccgccgtttc
       61 tgcaaggtct actagaggtt tactgatctg ctgggagatg tagacaccca gagccgagac
      121 caatgaagct acaccaagca tacccccaat caacaaccac agcaggcgct gctgcgagcg
      181 aacgatatca gagaccagga gggcgacttc tacggtcaca cccagatatt ccagcggtac
      241 cttttcggca taatagggtt gcccgtccaa tttgaactga ctttgctcaa ttccgcttgg
      301 tgagcctgca gcaatcagtt gatgaggaat gttggataga tcagataagt caaagtcaaa
      361 gctcgaggcg acaggtcgcc cactcaccaa aagtgtgttt tccaacccgc tgaaagcccg
      421 catttgggta gcaaacgcgt ggtctatacg gtttgctaca attacacgcc cgatagtatg
      481 gctatccatg acgataggat ggatggcaac catcaatgcc tgaggaggtt cctgactatc
      541 cacgaggtac attccaccaa tgtctttttc caaacagatc tgattggaga ccatattctc
      601 tgttgcggtg acaacattat catccacatc acaaaccaca atcagatcat atgctgcagc
      661 attcttaagg gtcaccaaat aatccaaaag atccctacgg ttatcttgac taaggaactc
      721 atgcaacgtt ggacgttctg cagtcaaggt ggcaaagtcg atgatctctt tctgctgagc
      781 cgcgcagaaa gcatctgcgg cgcgcactcc ctgctctacc acaatccacg cttgtcggtc
      841 taaatcatga cgaagcagcc atatcgtagg aacgccgatt gtcacggcag tcaggacaac
      901 aacgaaaaca aaacccagta tcatctgaac tggcaagctc agcctatcga aaattaccct
      961 gaacattcgg cctggctttt ccagcaaccg ctcgatagac aaaacagcct cgtttattcg
     1021 ttatcaatga tcttgtacac aatgtccccc ttgcgtacag gttctgcaac tttcagagca
     1081 acatccatcc cagggccaac cgtttgcact ttgcgatgct ctatctccat cgaagtaacg
     1141 acttgggtta gttccgatgt cctccccaag atcaagatct tatcgccgat ctctaagttc
     1201 cctgaaagtt ccaaaacagc cacgcttatc ctgttgtaaa aatgggttac tttaccgacg
     1261 cgtacctcca tcgcttcacc ctcccttctc cctaatcgtc aacacgttac atggaaaaga
     1321 ccgatgacac ccgtctttgc gctattccag gcttcaactg ctctcggtgt gggcattata
     1381 gctacccgga tggccttttc cctgaaaaag ttctcagcct cctctgagag tttgaccctt
     1441 ccgaattgac cagcgccgat cacaagctgt tccataccgt cttcataaat atgcctggct
     1501 tcgtcaagcg agatgatatg cgatgtgccg tagatcgcct tggagagttt tttcttgcgt
     1561 ttttcgatct tgccatccag acggatgatc acatcgtgct ggaattttgt cccagtgatc
     1621 gtgatagagc caaaattggt cgagtcaatt tgcgggttca tacttatagc ataagtgaaa
     1681 atttgaacaa aatcaagtga cttatttcag cattttctat aaaagactgt ctcattcacc
     1741 ccatgctcta aactccagcc ccacccaaga tatatctcaa tgagaaaaaa cgaaaatcgg
     1801 gttcgaaaag cgattaaaat agatccgctt tccattcgtc ggctgccagt atcgtttacg
     1861 ccatttatca ttctaatttt
//
LOCUS       DBNHOH010000792         1040 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_113675, whole genome shotgun sequence.
ACCESSION   DBNHOH010000792 DBNHOH010000000
VERSION     DBNHOH010000792.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_113675"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(315..980)
                     /locus_tag="AC16V7_14230"
     CDS             complement(315..980)
                     /locus_tag="AC16V7_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007694271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HIR6258327.1"
                     /translation="MKSFQKMGGIAALYEAAAYLIALVGYLLVVGTMDVVDPVERVAL
                     LVDNQAFLYMLNLVTYIIWGFFQIALALALYERLKAGSPALVQIATGIGLVWACVIIA
                     SGMIANVGMATVVDLYGKDPEHAATVWLAIGSVADGLSGASGETLGGTWVLLLSWVAL
                     RVGELPKALNYFGVVISVAGLLSTIPALFLALVFVFTLGKIVWLIWLGIVMLRSSPSA
                     TAA"
BASE COUNT          255 a          320 c          246 g          219 t
ORIGIN      
        1 tgatttggtc attttcagca tagtttacga ccatacatcc tgcaagcgtg caaaatctaa
       61 cgcccgagct gaggtgccgc ccagcttctc tgcgcaagac gtcactaact acgaagcttg
      121 agacaaaacg cgctgccgat tcctcccgcg cgaaagggca gcgcctccag cgaaatgttg
      181 ggtggcgctt tgtcaccgat ttttcgcaat gctatacaat ggctgagtat accgaaatcg
      241 gagtattggg tcaggtgtga gccgccttct ccaatactcc tactcgccgt tcggccttga
      301 agtcgaaagg atttttacgc cgccgtcgcg cttgggctac tgcgcaacat gacgatcccc
      361 agccatatca accacacgat cttgcccagc gtaaaaacga acactagcgc caggaagaga
      421 gctgggatgg tcgagaggag acccgccaca ctgatcacca cgccaaagta gttcagtgcc
      481 ttgggaagct ctcctacccg caaagctacc caacttaata gcagaaccca tgtgccgcct
      541 agagtctcac cacttgcacc gcttaatcca tctgccacag atccaattgc caaccagact
      601 gtcgcagcgt gctccgggtc tttgccatag agatcgacga cagtagccat gccgacgttt
      661 gcgatcatgc cgctggcaat gatcacgcag gcccagacaa gcccgatgcc ggtcgctatc
      721 tgcaccaatg caggtgagcc ggccttcaag cgctcgtaga gcgccagcgc cagggcaatc
      781 tgaaagaagc cccatattat ataggtgacc aggttcaaca tgtacaggaa ggcctggttg
      841 tccacgagca aagccaccct ctcgacaggg tcgacaacgt ccatagtgcc aactaccaag
      901 aggtaaccca ccagtgcaat caagtaggct gctgcctcgt acagtgcggc aataccgccc
      961 atcttttgaa aactcttcat aatacttctc ctccaattct agcttcccac ctcgcactgc
     1021 tttgcccctt gggtatgtcg
//
LOCUS       DBNHOH010000793         3902 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_691153, whole genome shotgun sequence.
ACCESSION   DBNHOH010000793 DBNHOH010000000
VERSION     DBNHOH010000793.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_691153"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="AC16V7_14235"
     CDS             complement(<1..618)
                     /locus_tag="AC16V7_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005189740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6258328.1"
                     /translation="MKTHQFDVVVVGAGGAGLMAALYASKTASTAVLSKLYPTRSHTG
                     TAQGGVGAALGNEEEDHPEWHTYDTVKGSDYLGDQDAIEFMCTEAVQAVYEMEHMGLP
                     FSRTSEGRIAQRPFGGHTNNVTGKPVRRACYAADRTGHMILQTLYQQCIKNKVNFFDE
                     FQVVDLILSNNAAAGVVAIELLTGEFHVFHAKSIIFATGGHGRIWE"
     gene            complement(643..1062)
                     /locus_tag="AC16V7_14240"
     CDS             complement(643..1062)
                     /locus_tag="AC16V7_14240"
                     /inference="COORDINATES: protein motif:HMM:NF013307.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258329.1"
                     /translation="MHARSVQPGVSFEYMMWIFTRISGLAMILLAIIGMSGAFILGAR
                     TQVDLPTLMRWSFFPNPNHVVNSNIPDVAVGWATAYWQIMQMMIFFFASTHAFNGLRV
                     VLEDFIGQSFLQPLLRGAIFLLWLFSLITAVFVILAS"
     gene            complement(1084..1473)
                     /locus_tag="AC16V7_14245"
     CDS             complement(1084..1473)
                     /locus_tag="AC16V7_14245"
                     /inference="COORDINATES: protein motif:HMM:NF013307.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     transmembrane subunit"
                     /protein_id="HIR6258330.1"
                     /translation="MAINRKVGFFQGLSYHGGGPMLSWMLHRVTGLGILAFVGLHVIS
                     SFFMQQTGSDIAAFINTIYESWVFQIFVAFFVLFHALNGLRIAVLDIWPKMLNYEREA
                     LWLQWFIFVPVYGLTVYLLIQRSLVGG"
     gene            1747..2232
                     /locus_tag="AC16V7_14250"
     CDS             1747..2232
                     /locus_tag="AC16V7_14250"
                     /EC_number="7.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="QcrA and Rieske domain-containing protein"
                     /protein_id="HIR6258331.1"
                     /translation="MTNSPHLDRRQFVNVVLTFLSAIIGAVLGLPAIGYLISPATKVQ
                     KKDEWITLGPLEKYQVGTPTLFSFNRTSINGWEKTVNSYGAYVVRFSPDNIKVFSNMC
                     THLSCRVNWKEDLKEYICPCHDGRFDYEGKITAGPPPQEMHEYETKIEDGNLFIHFLE
                     S"
     gene            2235..2894
                     /locus_tag="AC16V7_14255"
     CDS             2235..2894
                     /locus_tag="AC16V7_14255"
                     /inference="COORDINATES: protein motif:HMM:NF012263.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008121 - quinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HIR6258332.1"
                     /translation="MAAKIFAWIDERMGITSIYNLVLDRAVPKVNWWYTLGSASLFLF
                     ALQGVTGIFLAVYYVPSPDHAYESIQYIMNNVAFGWLIRGIHHWGASLMVLFVFIHML
                     RTFYYAAYKFPREITWLTGVVLFLATLGMGFTGYLLPWNQRAYWATTVGTEIAGSAPL
                     IGDFILRVLRGGNDLSAVTLARFFAVHIWFLPAIIALIIGVHLYLVIRLGISALPKEE
                     D"
     gene            2894..>3902
                     /locus_tag="AC16V7_14260"
     CDS             2894..>3902
                     /locus_tag="AC16V7_14260"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6258333.1"
                     /translation="MDEQQKESYLKKYKQDKSQGVKFYPDIIYKDLLASFAIFVLLVG
                     LAIFVGVKPEPPADPSDSAYIPRPEWYFLFLFELLKYFPGQLEWIGTVILPTIAILAL
                     FLLPFYDRSPFRHWKKRKLAVGVMGTIVLAIVALTVIAAVSTPPQEETNLATSLSESI
                     LLGGDLYSVHCVECHGADGEGGEIKGVEGLEGVVLKPINSQDEMYTRSDDTLYTIIDY
                     GQPDLGMPPFGVGYGGELRRGDTQAVVNFMRYTWDDRAEIPEDVTQASAIPTLAPNQV
                     PSYVEHIQPIFKRYCISCHRPGKKNNNYIMRTYEEVITTGDHAPNIISGNLESNTLLM
                     LD"
BASE COUNT         1004 a         1059 c          940 g          899 t
ORIGIN      
        1 ctcccaaatg cgaccatgac caccagtagc aaagatgatc gacttggcgt ggaagacatg
       61 gaactcacct gtaagcaact caatcgccac cacgccagcc gcagcgttat tggatagaat
      121 caggtcaacc acctgaaatt cgtcgaaaaa attgacctta ttcttgatgc actgctggta
      181 aagcgtttga aggatcatat gaccggtgcg atcagctgcg tagcaggcgc gccgcactgg
      241 cttgccagta acgttgtttg tgtgtccccc aaagggtcgc tgggcgattc gcccctcaga
      301 cgtacgcgag aagggcaagc ccatgtgttc catttcgtac acggcctgca ccgcctcggt
      361 gcacatgaac tcaatcgcat cctgatcgcc caggtaatca ctacctttaa cagtatcgta
      421 agtatgccat tcaggatgat cttcttcctc gttgcctaaa gctgctccca cgcccccctg
      481 agctgtgccc gtatgagaac gggtgggata cagcttactc agcacagcgg tgctggcagt
      541 tttggaagcg tacaaggccg ccatcagacc tgctcccccc gcgccgacga cgacaacatc
      601 aaattggtgg gtcttcatta aatgtcttct tctccttcag tctcaggatg ccaagatcac
      661 aaatacagcc gtaatgagcg agaacaacca aagcaagaag atcgcgcctc gcagcagagg
      721 ttgcaggaac gactgaccta tgaaatcttc aaggacaacg cgcagtccat taaaagcgtg
      781 cgtagatgcg aagaagaata tcatcatctg cataatttgc caataggcag tcgcccaacc
      841 cacggctaca tcggggatgt tgctgtttac cacgtggttt gggttaggga agaatgacca
      901 acgcatcaag gtaggcaaat cgacctgcgt gcgcgccccc aggataaaag cgccgctcat
      961 cccaatgatc gccagcaaga tcatcgccag ccctgaaatg cgggtgaaga tccacatcat
     1021 gtattcaaag ctcacgccgg gttgtacact tcgagcatgc atattacacc tcctggctag
     1081 attttagccg cccaccagcg aacgctggat aagcaagtac actgtcaaac cgtagaccgg
     1141 cacgaagatg aaccactgca accacaaggc ttcccgttcg tagtttagca tcttcggcca
     1201 gatatccaaa acagcgatgc gcagcccgtt caaggcgtgg aacagcacaa agaatgccac
     1261 aaagatctgg aatacccagg attcgtaaat cgtgttaatg aacgcagcga tatcgcttcc
     1321 ggtttgctgc atgaaaaacg aggaaataac atgcaggcca acaaacgcca ggatgcctag
     1381 cccggtaacg cggtgcaaca tccaggaaag catgggtccc ccgccatgat acgaaagccc
     1441 ctgaaaaaag ccgactttcc gattgatagc catagcgcct cctatcactc atcttctatg
     1501 atgttcagaa tctacacagt agggagttaa tgtttggagc gcaacttccc cgcaatgttt
     1561 cttcaactgc gtaaatccga ctttaatagt atatctatta ttgctatacg agtgcacaag
     1621 taatagtgac aattatcaca gatatttagt ttcatgtgtc ctttgaactt atccttattt
     1681 tatctaaaat agaaaggtat ttgaacatat taatcgcaca aatcatcgag caaaagggga
     1741 attcgcatga ctaactcacc ccatcttgat cgccgccagt tcgtcaatgt cgttttgaca
     1801 ttcctgagcg ccatcattgg ggctgtgctg ggattgccag caataggcta cctgatctcg
     1861 ccggctacca aagtccaaaa gaaagacgag tggatcaccc tcgggcctct agagaaatat
     1921 caagtgggca ctccaactct attcagcttc aaccgcacct ccataaatgg gtgggaaaag
     1981 accgtcaaca gttatggggc atatgtggtt cggttcagcc cagataacat aaaagtattt
     2041 tccaatatgt gcacgcatct gagctgccgg gttaactgga aagaggacct taaagaatat
     2101 atttgcccat gccacgatgg acgcttcgac tatgagggta agatcacggc aggcccaccg
     2161 ccgcaagaaa tgcacgaata cgagaccaag atagaggatg gcaacctgtt catccatttc
     2221 ctagagagtt agccatggct gcaaaaatct tcgcctggat cgacgaacgt atgggtataa
     2281 cgtcgatcta caatctcgtt ctggaccgtg ctgtgcccaa ggtgaactgg tggtacacct
     2341 taggcagcgc ctcgctcttc ctgttcgccc ttcaaggagt aaccggcatc ttcctggcag
     2401 tatattacgt gccatcgccc gaccatgcct acgagagtat ccagtacatc atgaacaatg
     2461 ttgcgttcgg ttggctgatc cgaggcatcc accattgggg tgccagcctg atggtgctct
     2521 ttgtgtttat ccatatgctg cgcacatttt actatgcagc ctacaagttc ccccgagaga
     2581 tcacctggct gaccggcgtc gtgctgtttc tggcgacctt gggcatgggc ttcactggat
     2641 acctgctacc ctggaaccag cgggcttact gggccaccac agtcggcaca gaaatcgctg
     2701 gctcagcacc attaatcgga gattttatcc tgcgcgtcct gcgcgggggc aacgacctga
     2761 gcgccgttac cctggctcgc ttcttcgctg tccatatttg gttcctgcca gcaatcattg
     2821 ccctaatcat cggcgttcac ctttacctgg tcattcggct aggtatctcg gcgttgccaa
     2881 aagaagaaga ttaatggacg agcaacagaa ggaaagctat ctaaaaaagt acaagcaaga
     2941 taagagccag ggggttaagt tctaccccga catcatttat aaagaccttt tggcatcctt
     3001 tgctatcttc gtgctgctgg ttgggctggc gattttcgtc ggtgtgaagc ctgagccacc
     3061 ggctgatccg agcgattcag cctacatccc aagacccgaa tggtactttc ttttcctatt
     3121 cgagcttctc aagtacttcc cggggcaact ggaatggatc ggcacagtta tcctgccaac
     3181 catcgcgatt ctggcgctat ttctgctacc cttttacgat cgctccccat tccgccactg
     3241 gaagaagcgc aagttggcgg tcggtgtgat gggcaccatc gtcctcgcca tcgtagcact
     3301 aaccgtcata gccgctgtct ctacgccacc tcaagaggaa accaatcttg caacatccct
     3361 aagtgagagc atcctgctgg gaggagactt gtattctgtc cactgcgttg agtgccatgg
     3421 agcggatggc gagggcggag aaatcaaggg ggtggaagga ctggaagggg tagtgctcaa
     3481 gcccatcaat agccaggacg agatgtatac ccgctccgac gataccctgt acaccatcat
     3541 tgattatggc cagcccgacc tgggcatgcc gcctttcggc gttggttacg gtggggaact
     3601 acgccgcggc gatacacagg ctgtggtaaa cttcatgcgc tacacctggg acgaccgcgc
     3661 cgagattcca gaggacgtga ctcaagctag tgccatcccc accctggctc caaaccaggt
     3721 gccgtcttat gtcgaacata ttcaacccat cttcaagcgc tactgcatct cttgtcatcg
     3781 acctggaaaa aagaacaata attacataat gcgcacgtac gaggaggtca tcaccacggg
     3841 cgaccatgcc cctaatatca tctcaggtaa tttagagagt aacacgctgt tgatgttaga
     3901 tc
//
LOCUS       DBNHOH010000794         1665 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_561831, whole genome shotgun sequence.
ACCESSION   DBNHOH010000794 DBNHOH010000000
VERSION     DBNHOH010000794.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_561831"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="AC16V7_14265"
     CDS             complement(<1..194)
                     /locus_tag="AC16V7_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258334.1"
                     /translation="MKLPIIKRAALLLVAQVAALMLMACYLVQALQPDGVTPSAPVPL
                     AQVVDLPTVVLPSPTNVPTET"
     gene            complement(206..565)
                     /locus_tag="AC16V7_14270"
     CDS             complement(206..565)
                     /locus_tag="AC16V7_14270"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6258335.1"
                     /translation="MPTRIAVISLWAEDVPKAAHFYRDVIGLPLLPHHADRPHFDLGG
                     SMLVILKGRPSPPLNSVPERFPLVAFAVDNLDAALQQLDQHGVELPWGVEADAHSRWA
                     MFYDPAGNLIEIVQFGR"
     gene            complement(576..1004)
                     /locus_tag="AC16V7_14275"
     CDS             complement(576..1004)
                     /locus_tag="AC16V7_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014933919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR6258336.1"
                     /translation="MEPPILPRRIPQRAEVGAKASFSRTITEADVALFIGVTWDVNPL
                     HTDEVYARATRFKQRIVPGLLTASLVTHLGGLWAFMASEIRFEFLAPVYVGDTITAVA
                     EVTEVKPDNGFVRLKVKCTNAAGKEVLRGGLAGFPGRHEA"
     gene            complement(1043..1501)
                     /locus_tag="AC16V7_14280"
     CDS             complement(1043..1501)
                     /locus_tag="AC16V7_14280"
                     /inference="COORDINATES: protein motif:HMM:NF016840.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR6258337.1"
                     /translation="METFFTDYLDRLESLHNDLKGALAEMPNGGLDWVPGAGMNSLCV
                     LAVHVAGAERYWIGDVVAGEPSGRDREAEFRAKGMTAASLAERLDGSLAYTRGVVQRL
                     KPSDLDEIRFSPQHGRENAVGWVLAHVLAHAGLHAGHAQVTRQLWEQKNT"
     gene            complement(1482..>1665)
                     /locus_tag="AC16V7_14285"
     CDS             complement(1482..>1665)
                     /locus_tag="AC16V7_14285"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6258338.1"
                     /translation="YGFETCGLARIVARAMRENRASIRVIEKLGMQFDKEFIENGAFW
                     VQYEIYEEKVNGNFLH"
BASE COUNT          378 a          524 c          419 g          344 t
ORIGIN      
        1 gtttccgttg ggacattcgt aggtgagggc agtacaacgg taggcaaatc gacaacctgc
       61 gccagcggaa cgggcgcgct gggagtgaca ccatccggtt gaagcgcttg aaccaaatag
      121 caagccatta acataagcgc agcgacctgg gcgacgagca ataaggcggc gcgtttaatg
      181 ataggtagtt tcatgtctgt cccccttagc gaccaaactg cacaatctca atcaggttcc
      241 ccgctggatc ataaaacatt gcccagcgag aatgcgcgtc cgcctctacg ccccagggca
      301 actcgacacc atgttggtca agctgctgga gggctgcatc cagattgtct accgcgaaag
      361 cgaccaatgg gaagcgctct ggcacgctat tcaggggcgg agatgggcgt cctttcaaga
      421 tcaccagcat actgccaccc agatcgaaat gcgggcggtc ggcgtgatgg ggcaataacg
      481 gcaagccgat gacatcccgg taaaagtggg cggctttggg cacatcctcc gcccacaggc
      541 tgattacggc aatacgggtt ggcatttttt ctccttcaag cttcatgacg accagggaag
      601 ccagccagtc ccccgcgtaa cacctctttc cccgcagcat tcgtacactt gaccttaagg
      661 cggacaaacc cgttatcggg tttgacttcg gtcacctcag ccacggctgt gatcgtgtcg
      721 ccaacataga ccggcgccag gaattcgaag cgtatctcgc tcgccatgaa cgcccatagt
      781 cctcccaggt gggtgaccag gctagcagtg agcagcccag gtacgatgcg ctgcttgaaa
      841 cgcgtagcgc gtgcgtacac ctcatcggtg tgcagcgggt tgacatccca ggtaacccca
      901 ataaacaagg ctacatccgc ctcggtgatg gtgcgcgaga agcttgcctt agcacccacc
      961 tcagcgcgct gcgggatgcg tcttggtaat atcggaggtt ccatagaatc tatttaagtt
     1021 gtataacgct ccccacctgc tgtcaagtat ttttctgctc ccacaactgc cgcgtcacct
     1081 gggcgtgacc cgcgtggagc cccgcgtgcg ccagcacatg ggccaatacc cagccgacgg
     1141 cgttctcgcg cccatgttgt ggggaaaagc gtatttcatc caggtcgctt ggcttcaacc
     1201 tctgaaccac gccacgcgta taagccaggc tgccatccaa acgctctgcc aagcttgccg
     1261 cggtcatacc cttcgcacgg aactcagctt ccctatcacg cccagatggc tccccagcga
     1321 ccacatcccc aatccagtag cgttccgccc cagccacatg aacggccaaa acgcacagcg
     1381 agttcattcc agcccctgga acccaatcca gcccgccatt cggcatctca gccagggcgc
     1441 ctttcaggtc attatgcaaa ctctcaagtc gatccaggta atcagtgaag aaagtttcca
     1501 ttcactttct cctcataaat ttcgtactga acccaaaacg ctccattctc gataaactct
     1561 ttatcaaatt gcatgcccaa tttttctatg accctgatgg atgcccggtt ctcccgcatg
     1621 gcgcgcgcca cgatgcgtgc cagcccgcat gtctcgaagc catac
//
LOCUS       DBNHOH010000795         3058 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1461234, whole genome shotgun sequence.
ACCESSION   DBNHOH010000795 DBNHOH010000000
VERSION     DBNHOH010000795.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1461234"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="AC16V7_14290"
     CDS             complement(<1..345)
                     /locus_tag="AC16V7_14290"
                     /inference="COORDINATES: protein motif:HMM:NF027889.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp1/GlmU family protein"
                     /protein_id="HIR6258339.1"
                     /translation="MVVGAPDTGKSTFARYLFKRLSGEGLRVAYLDGDPGQSTLGPPT
                     TLSLVIAHPGERRFPPAGQIWRWFIGSVSPHGHMLPLLVAAARLVRVGLEGGADVVVY
                     DTCGLIDPNQGGM"
     gene            complement(425..1273)
                     /locus_tag="AC16V7_14295"
     CDS             complement(425..1273)
                     /locus_tag="AC16V7_14295"
                     /inference="COORDINATES: protein motif:HMM:NF015690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HIR6258340.1"
                     /translation="MAFEDKPLIMAFVSDLYFSSRIESVTQRMGFTVLFIERDEEDEP
                     NNLGDVDIQYAEHLDGQGGALLDKLTQQLPALIIFDLGNADIAWRKWILLIKSSPATR
                     RTPVLCFGSHMDTESMKAAKAAGADAVLARSKFVQSLSELIQKYARRIDYGALKDTCQ
                     HSLSEIAVRGLEEFNRGEYFEAHESLEDAWNQDQTAGRELYRAILQVAVAYLQIERRN
                     FRGAMKMFLRLRQWIDPLPDMCRGINVAQLRSDALQVHEALKALGPEHINEFDHSLFA
                     PVLYFR"
     gene            complement(1278..2180)
                     /locus_tag="AC16V7_14300"
     CDS             complement(1278..2180)
                     /locus_tag="AC16V7_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258341.1"
                     /translation="MPFEIQAEAVLRGIERFAEGLRFAPADLKADQLIRRLKRVYVPS
                     WLVDVDVQATWELEAGFDYQVVSHQDRYNEIGGGWTSQQVIEGRIRWEPRVGRLTRTY
                     QNISAPAIEDDREIRQNLGAFDRSKAEAYQEVAIKDSFIRLPERETQDAWTDAVPEIQ
                     GVASQECLEAAGADHVRSFGWTPKYLNHNWSLLLQPIYTTYYLDDENQPQLVYVHGQS
                     AKISGVRRASMKRAQRLALMILGAAIFMAFLSLVITAIGVVAPPLLVIGGLGLGASVF
                     VGLGALLPLVIAWQFNRNQKGMAK"
     gene            complement(2408..>3058)
                     /locus_tag="AC16V7_14305"
     CDS             complement(2408..>3058)
                     /locus_tag="AC16V7_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258342.1"
                     /translation="RYFQDGEWKTRTVIRWRWESGKVNEKFDDLLVPGTNRLSSLLLN
                     KIKAFQMSQLRPYEAGFLAGFQALSYDRPLDNSWEIARQQMREHTRLACRKQASTPRI
                     RNFSMKLDFGNESWRYILLPIYLAGYRYANQNFQVIINGQSGQIAGQRPVDWKKVWLV
                     VGALLTPGLLLALIGAITLLLGGVGALIGGVGFVLLLIGVVISVVILRQATSMDDI"
BASE COUNT          816 a          850 c          638 g          754 t
ORIGIN      
        1 cattccgccc tgatttggat cgatcaaccc acatgtatcg tataccacca catccgcacc
       61 accttccaac ccaactcgaa ccaggcgcgc agcagccacc aaaaggggta acatgtgccc
      121 atgaggcgag acagatccaa tgaaccaacg ccaaatctga ccggctgggg gaaatcgcct
      181 ttctccagga tgtgcaatta ccagcgaaag tgttgtagga ggcccgagcg tgctctgccc
      241 ggggtcgccg tccaaatatg ccactcttaa gccctctccg ctcaagcgtt tgaacagata
      301 acgagcaaag gtgctcttcc ccgtatctgg tgctccaaca accattaaga cccctcttag
      361 agatcccaaa tcgagttgtc cccaggtttc gggaattata ggatcgggtt ggctcacgag
      421 ttgatcacct gaaatagagc acgggggcaa ataggctgtg atcaaactcg ttgatatgct
      481 ccggccctaa agcctttaga gcttcatgca cctgtagagc atcagatcgt aattgtgcca
      541 cgtttatccc ccggcacata tctggaagag ggtcaatcca ttggcgcaag cgcaggaaca
      601 tcttcatggc accacggaaa tttcttcgtt caatttgtaa gtacgccact gcgacttgca
      661 atatggcacg ataaagctca cgccctgcag tttgatcctg attccaggca tcctccagag
      721 actcatgtgc ctcaaaatat tctccccggt tgaactcctc caaaccacgc acggcgattt
      781 cggaaagcga gtgctggcaa gtatctttca acgcgccata atcaattctg cgggcatatt
      841 tttggatcag ctcactcaat gactggacaa atttagagcg agccagcact gcgtccgcac
      901 ctgctgcctt tgcagctttc atgctctccg tatccatatg tgatccgaaa cacaacactg
      961 gtgttcgccg cgtggccgga gacgatttga tcaagagaat ccattttcgc caagcgatat
     1021 cagcatttcc tagatcaaaa atgatcaatg ccggaagctg ctgggtgagc ttatccaata
     1081 gtgccccccc ttgcccatcc agatgttcag catattggat atcaacatcg cccagattgt
     1141 ttggctcatc ttcctcgtct cgttcaataa ataacacggt aaaacccatc ctttgggtta
     1201 ccgactcgat cctggatgag aaatataaat cgctaacaaa agccatgata agaggtttat
     1261 cttcaaaagc catctgctta ttttgccatc cccttctgat tgcggttgaa ctgccatgca
     1321 atcactaaag gtaaaagtgc tcccaatcct acaaatacag aagctcccag acctaatccg
     1381 ccgataacta gtaaaggcgg tgccactaca ccaatcgcag tgattacaag gctaaggaaa
     1441 gccataaaaa ttgctgcccc caagatcatt aaagctagcc gttgcgcacg cttcatagat
     1501 gcccgccgaa ctccgctgat ttttgcactt tgcccatgaa catacacgag ctggggctgg
     1561 ttttcatcgt ccagataata ggttgtatag attggctgca agagaagaga ccaattatgg
     1621 ttcaaatatt tgggcgtcca accaaaactt ctcacatgat cagctcccgc tgcttccagg
     1681 cattcttggc tagcaacacc ttgtatttct ggaacagcat ccgtccaggc gtcctgtgtt
     1741 tcacgttctg gcaaacggat aaacgagtct ttaatcgcta cttcctgata tgcttcggct
     1801 ttactacgat cgaaagcgcc taaattttgg cgaatctctc gatcatcttc aattgcaggc
     1861 gcagagatat tttggtaggt gcgcgtcaat cgacctaccc gaggctccca acgtatacgc
     1921 ccctcaataa cctgctggga ggtccatccc ccaccgatct cattgtaacg atcctggtga
     1981 ctgactacct gataatcgaa accagcctct agctcccagg tagcttgcac gtctacatct
     2041 accaaccagg agggaacata tacccgttta agcctcctaa taagctggtc agccttcaga
     2101 tccgcaggag caaatcttag accctcagca aaacgctcaa tccctcgcaa taccgcttcg
     2161 gcttggatct caaatggcat tactagctca ggaggtttta gatcaggaaa ttggctgctg
     2221 tcctgctcaa tgagcgcaag cgcctcatga aagcagtgag gacagggctt ggtcataaga
     2281 ttttttggag ccaaatatgt ccattcacac ttttggcaaa aaatcaactc tgcgtcaaag
     2341 ccccaaactg aagcaagatt ctcaaattgg gcaagttctc tcgagtcttc tcccatggat
     2401 accctcttta gatatcatcc atactcgtag cctggcgaag gatcacaact gagattacca
     2461 caccgatgag cagcaataca aagccaacac ctccgattag agcgcctact cctccaagta
     2521 gcaaggtaat ggctccgata agtgccagaa gtaatcctgg cgtgagcaga gcaccaacta
     2581 ccaaccacac ttttttccaa tccacggggc gttgccccgc aatttgcccg ctttgcccat
     2641 tgatgatgac ctggaagttt tgattagcat aacgataacc cgccaagtag atcggcagca
     2701 aaatataccg ccagctctca ttcccgaaat ccagtttcat actgaaatta cggattcgtg
     2761 gtgtactggc ttgttttcta catgccaggc gcgtatgctc tcgcatctgc tggcgagcta
     2821 tctcccatga attatccaga ggccggtcat aagataaagc ctgaaatcca gccagaaatc
     2881 cagcttcata aggtctgagc tgactcatct ggaaagcttt tatcttattt agcaaaaggc
     2941 tactcaacct atttgtccca ggaaccagta aatcatcaaa tttctcattc acctttccag
     3001 actcccaacg ccatctgatt acagtgcgcg ttttccactc tccgtcttga aaatagcg
//
LOCUS       DBNHOH010000796         2648 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1461289, whole genome shotgun sequence.
ACCESSION   DBNHOH010000796 DBNHOH010000000
VERSION     DBNHOH010000796.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1461289"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="AC16V7_14310"
     CDS             <1..843
                     /locus_tag="AC16V7_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HIR6258343.1"
                     /translation="TFATDQVGWYAVQVNCNDIATTGATPLWFLATILLPEKHATAKM
                     AEEILTQVFDACKTIGVAVIGGHTEITFGIERPIVVATMVGEVARERLVTPRGATPGD
                     RILLSKGVPIEATSILARELPDRLGDFLDEVELRQAEMFLFEPGISILREARLAVSAG
                     KVNAMHDPTEGGLITALWELAEASGQTLWVDLSAVPVPSISRKMCQMLGIDPLAAIAS
                     GALLLTAPPKDAEMIRSAWEKEGILCSEIGGVEEGPAAVWEVRESGRQLLWKPVRDEI
                     AKLF"
     gene            complement(851..2221)
                     /locus_tag="AC16V7_14315"
     CDS             complement(851..2221)
                     /locus_tag="AC16V7_14315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6258344.1"
                     /translation="MNSSADVIVCGAGIAGISAAYHLSVKHGMKNILLIDDRPPLSLT
                     SDKSTECYRNWWPGPGDAMVALMNRSIDLMEELARQSNNIFHLNRRGYLYLTADPANL
                     GKFVNAASEPPTLGAGDLRVHQGKAGDPEYHPATAEGFENQPTGADLILEPRLIRKHF
                     PYLSDKVIAALHVRRAGWFSAQQLGTYLLDQARSHGVRLHNAQVTRVETVSGRVNSVI
                     LNTGEKLATRNFINASGPMLKNVASLIGIELPIYCELHLKVAFKDTLGVILRDAPLLI
                     WTDSQFLPWSPEEESILFKDESLHWLLENMPSGVHTRPEGSANSEILLMLWEYQQHKT
                     DPVFPPPLDDQYPDIVLRGLATMIPGLKAYFHRAPRPVLDGGYYTKTLENRPLIGPLR
                     VDGAFVIGALSGYGLMAACAAGELLAAHLTGDALPPYAPAFTLDRYLDPTYQQQISNW
                     KDSGQL"
     gene            complement(2218..>2648)
                     /locus_tag="AC16V7_14320"
     CDS             complement(2218..>2648)
                     /locus_tag="AC16V7_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0002196 - Ser-tRNA(Ala) deacylase activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HIR6258345.1"
                     /translation="AMHVLCGVIFRDYGASVTGGDMDPLKGRMDFEFETLQRDLVDDI
                     QSSINSEVDNARPVRIKILPREEAFKIPDLIRTKINLLPEGISQVRVVEIVGLDLQAD
                     GGTHVANTSEVGFIRVVDYKSKGKINKRIYVEVGDKETIE"
BASE COUNT          657 a          649 c          688 g          654 t
ORIGIN      
        1 accttcgcga ccgaccaggt cggctggtat gccgttcagg ttaattgcaa tgatatcgct
       61 accacagggg ccaccccgct ctggttttta gcgacaatat tgctgcctga gaaacatgca
      121 accgcgaaga tggcagaaga gatcttaacc caggttttcg atgcttgtaa aacaatcggt
      181 gtcgcagtca ttggaggcca tacagaaatc acatttggga ttgaacgtcc aattgtagtg
      241 gctacaatgg tgggcgaggt tgctcgcgag agactggtaa cgccccgcgg agcgacacca
      301 ggagatcgca tcctgctaag caagggggtt ccgatcgagg caacctctat acttgctaga
      361 gaattgccag accgattggg ggatttttta gacgaagtcg agctcaggca agcagaaatg
      421 tttttattcg aaccaggtat cagcatcctg cgagaggcgc gactggcagt ttcggccggc
      481 aaggttaatg cgatgcacga tccaaccgaa ggtgggttga ttactgctct ctgggaactg
      541 gcagaagcca gtggccagac gctgtgggtg gatttgagcg cagtaccagt cccgagcata
      601 tctagaaaga tgtgtcagat gcttggcatt gatccattag ccgctattgc atcgggtgcc
      661 ttgctgctaa ctgcccctcc aaaggatgca gaaatgatcc gaagcgcctg ggagaaagaa
      721 ggaatcttgt gttccgagat tggcggggtg gaggagggac ctgcggcagt atgggaagtg
      781 agagaatccg gcaggcaatt actctggaaa cctgtacgag atgagatagc taagttattc
      841 taagctcgcg tcagagttgc cctgaatctt tccagttact gatctgttgt tggtatgtgg
      901 ggtctaggta cctatctagc gtgaacgcgg gcgcataggg aggcaaagcg tctccagtca
      961 ggtgagcagc gagtagctcc ccagccgcgc aagccgccat caaaccgtac ccagagagcg
     1021 caccgataac aaacgcccca tccactcgaa gtggtccgat cagcggccga ttttcaaggg
     1081 tcttggtgta atagcctcca tcaagtacag gacgaggtgc tctatgaaaa tatgccttca
     1141 gacccgggat catggtcgct agaccacgca gaacaatatc gggatattga tcatctaggg
     1201 gaggaggaaa gacaggatcg gttttatgtt gttgatattc ccagagcatc agcaaaatct
     1261 cgctatttgc actgccttct gggcgggtgt ggacaccaga cggcatattc tctagcaacc
     1321 aatgcaagct ttcgtcttta aaaagtatag attcttcttc aggcgaccag ggaagaaatt
     1381 gagaatctgt ccagatcagt aaaggtgcat cgcgcaatat tacgcccagc gtatctttga
     1441 atgccacttt taagtgcaat tcgcaatata ttggcagttc aatcccgata agactcgcaa
     1501 cattctttaa catggggcca ctggcattga taaagtttcg cgtagccaat ttctcgccag
     1561 tgtttaatat taccgaattt actcgaccgc tgacagtctc cacgcgagtt acctgcgcat
     1621 tgtgcagcct gacaccatgc gaacgagcct gatctagcag ataagtgccg agttgttggg
     1681 cgctgaacca tcccgcccgg cgcacatgta gggctgctat caccttgtca gagagatatg
     1741 gaaaatgttt ccggatcaga cggggttcaa ggattaaatc agcacccgta ggctggttct
     1801 caaacccttc ggcagttgca gggtggtatt cgggatctcc tgctttacct tggtgtacgc
     1861 gcagatcacc agcacccagt gtgggtggct cggaagctgc attcacaaat ttgccgagat
     1921 ttgctgggtc tgctgtcaaa tataggtatc cccggcggtt tagatgaaat atattattac
     1981 tttggcgagc cagttcttcc atcaggtcga tgctgcgatt catcagtgct accatcgcgt
     2041 ctcccggtcc aggccaccaa tttcggtaac actcagtgga tttatcgctg gtgaggctaa
     2101 gaggggggcg atcatcgatc agaagtatat ttttcatgcc atgcttcacc gataaatgat
     2161 aagcggcgct gatgcctgca atgcccgcgc cgcatacgat gacatctgcg ctggaattca
     2221 ttctatcgtt tccttgtcac cgacttcgac ataaatacgt ttgttgattt ttcctttact
     2281 tttgtaatct accacgcgga taaagccgac ttctgacgtg ttggctacat gtgtgcctcc
     2341 atcagcttgc aaatccaggc caacgatctc gacgacacgt acctgcgaga ttccctccgg
     2401 tagcagattt atttttgtac gaatcaggtc tggaatctta aacgcttctt cgcgcggcag
     2461 aatcttgata cgtaccggac gagcgttatc aacttccgag ttgatcgagc tctgaatatc
     2521 atccaccaaa tctctttgca aggtctcgaa ttcaaaatcc atgcgaccct tgagggggtc
     2581 catatcgcca ccagttaccg aggcgccata atcccggaaa attacaccac aaagaacatg
     2641 cattgccg
//
LOCUS       DBNHOH010000797         2066 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_370911, whole genome shotgun sequence.
ACCESSION   DBNHOH010000797 DBNHOH010000000
VERSION     DBNHOH010000797.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_370911"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="AC16V7_14325"
     CDS             complement(<1..684)
                     /locus_tag="AC16V7_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6258346.1"
                     /translation="MTKITDATPLTNVPSDSDSLSPEDLQHVLEIPLWQVRLRLFWRG
                     LRTNVGLFAENPIGLVGLGVILFFILLMVAHPILMDTVWDKNTYDPVLGFDVSVPEHP
                     APPSTTHLLGTDPLGRDVLSQLMWSTRSAFVLGLLTALVTVFFATTIGAVSAYYGGII
                     DTLFMRLADIVIMTPLITILIVMAALFDLGIIHLAIILGLLNGFGGTTIIIKSQALTV
                     KVRTFIRSEE"
     gene            complement(713..1864)
                     /locus_tag="AC16V7_14330"
     CDS             complement(713..1864)
                     /locus_tag="AC16V7_14330"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6258347.1"
                     /translation="MRRYVIRRLGQNLITLFLFLTMVYLLLDAQPGDFGNIYASDPRL
                     TPAQRQQLRANLGLDKPVLVRYGIWLGNVVRGDFGLSYSNYPRKVLDIIVERAPRTVI
                     LFISIQILAYYIGFLMGKILAWRRGGTLEYVSTIVGVGLYTVFVPWFALMMIWFFAYT
                     LDLFPIGKFIDPVLWQGSPVDANYIFNRMLLTALIASVILLVLAFYARKLNPGQRRPA
                     LWVGLVVVLAGVLIYWISTGVGRYAVDILHHLVLPIFTLATVNFAGIMLLTRNSMLET
                     LREDYIMAARAKGLPDKVVRDKHAARNTMLPVATSFVLAISFVLDGGIITETIFSWPG
                     MGRTLLIAAQAQDIPMVIGALLFTGVLALMGHLFADVLYAFLDPRIRYS"
BASE COUNT          539 a          562 c          587 g          378 t
ORIGIN      
        1 ctcttccgat ctgataaagg tgcgcacctt gaccgtgagc gcctgggatt tgatgataat
       61 ggtggtgcca ccgaagccgt tcagcaagcc caggatgatc gccaggtgta tgatgcctaa
      121 atcaaacaac gccgccatga cgatcaggat agtgatcagc ggggtcataa tgacgatgtc
      181 agccaggcgc atgaacagcg tatcgatgat acccccgtaa taagctgaca cggcccctat
      241 ggtggtggcg aagaagaccg tgaccagggc ggtgagcaag cccaacacaa aggctgagcg
      301 cgtgctccac atcagctggc tgagcacatc tcgacccagg ggatcggtac ccagcaggtg
      361 ggtggtagac gggggagccg gatgctctgg aacgctgaca tcgaatccca gcaccgggtc
      421 gtaggtgttt ttatcccaaa ccgtgtccat taagatcgga tgggcgacca tcaaaaggat
      481 aaagaacaag atcactccca gcccaaccag tccaatcggg ttctcggcaa acaggcccac
      541 attggtgcgc aacccccgcc agaaaagcct caagcgcact tgccacagcg ggatttccag
      601 cacatgttgc aggtcttcag gcgagaggct atcagaatcc gagggcacat tggttaacgg
      661 agttgcatcc gtaattttcg tcattattct tcaacctcca tttcactggt acctatgagt
      721 aacggatgcg cggatcgaga aaggcgtata agacgtccgc aaacaggtgg cccatcaatg
      781 ccaggacacc ggtaaaaagc aaggcgccga tgaccatggg gatatcctgc gcctgggcgg
      841 cgatgagcag cgtgcgcccc atgcccggcc aggagaagat ggtctcggtg atgatgccgc
      901 catccagcac gaaggaaatc gccagcacga aggaggtggc taccggcagc atggtgttgc
      961 gggcagcgtg cttatcgcgg accaccttat ccggcaaccc cttggcgcgg gctgccatga
     1021 tgtagtcctc gcgcagcgtt tccagcatgg aattgcgcgt cagcagcatg atcccggcga
     1081 agttgactgt ggccaatgta aatatgggca gcaccagatg gtgcaaaatg tccaccgcgt
     1141 agcgtccaac cccagtcgaa atccaataaa tcaagacgcc agccaagaca acgaccagac
     1201 caacccacag ggctggacgg cgctggcctg ggtttaactt gcgggcgtag aatgccaata
     1261 cgagcagaat aacgctcgcg ataagtgcag tcaataacat gcggttgaaa atgtaattag
     1321 cgtccaccgg cgagccttgc cacaggactg ggtcaataaa tttgccgatg gggaacagat
     1381 ccaacgtgta ggcgaagaac cagatcatca tcagggcaaa ccagggcaca aagacggtgt
     1441 acagtcccac accaacgatg gtagagacgt actccaaggt gccacctcgc ctccaggcaa
     1501 ggatcttgcc catcagaaaa ccgatatagt aagccaagat ttggatgctg atgaacagga
     1561 tcaccgtgcg cggggcgcgc tcgacgatga tatccagcac cttgcgggga tagttcgagt
     1621 aagacaagcc aaagtctccg cgcacgacgt tccccagcca gatgccgtag cgcaccagca
     1681 cgggtttatc caagccgagg ttggcgcgca gttgctggcg ctgggcaggt gtcaggcgcg
     1741 ggtcactggc atagatattg ccaaaatcac ccggctgggc gtccaacagc aggtagacca
     1801 tggtgagaaa taaaaacagg gtgatcaggt tctggccaag cctgcggatt acataacgtc
     1861 tcattaaatc actcctgaaa atccagggat actggggagg gcaggcctag gcgacctgcc
     1921 ctccttggat aggccttaca gacctacagc agttgcaagc agaccagtct caattacttg
     1981 acttggacct gagtctcaag tccacccaag aatccaaact ggtgagcata ttgcagaccg
     2041 ctgagcgact tggtgtacgg gtattc
//
LOCUS       DBNHOH010000798         8419 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1461448, whole genome shotgun sequence.
ACCESSION   DBNHOH010000798 DBNHOH010000000
VERSION     DBNHOH010000798.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8419)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8419)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8419
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1461448"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1197)
                     /locus_tag="AC16V7_14335"
     CDS             complement(61..1197)
                     /locus_tag="AC16V7_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6258348.1"
                     /translation="MKLLLGINPRWDKLANFFMPILAALAALAVGAVMLLLLGVNPLV
                     AYSALLEGAFGSQNALADTLVKATPLLLVGIGICIAFRGGVINIGGEGQLVVGAIAST
                     LVGLSFTEAPGWLIIPLALLAGFLSGAIWGGIPGVLKAYFNVNEILSTIMMNQIAVQG
                     MNFLLRGPMIDPVQLEAASKIPQTARLSRLFDLPRLVPTRLHLGSALAVIMAILIFIF
                     LWRTITGYRIRAVGLNPHASRYAGIHVQRYVVLALLLSGALGGLAGAVQVFGVHHRMF
                     TDGSATGFTGSAGFNGIVAALFGQLHPLGTIPASFLFGALIVGANAMQRAVQVPSALI
                     TALNGLLVVFVVSTDLWRRRLQRRQLSEAIREEAAQVKEVKAAS"
     gene            complement(1214..2191)
                     /locus_tag="AC16V7_14340"
     CDS             complement(1214..2191)
                     /locus_tag="AC16V7_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6258349.1"
                     /translation="MNEIEFVRTMASIILASSPLIIAVCGETITERSGVVNLSLDGTM
                     LMAAMTGFVVGLKTDNVWLGFLAGAIIGAIFGAIVAFSSISLGKSQVAVGFVLTLLGD
                     DLSAFLGQNFTRIPGALVTRFGIPFLEDIPFLGPILFKQDIVVYFAYALVIFTWWFLN
                     RTQPGLHLRSSGERPEAAFARGVNVRRVRYLYALVGGALVGVAGAAYSLDVKIGWSDG
                     HTRGLGWIALAIVIFGGWSPIRGALGAILFGATKALATILQRTFPEVSVVAFNAIPWV
                     LMIAVLLVVSSDVTERIVSVMPNRIQPPLRRMLRINAPLALGSSLAEKG"
     gene            complement(2188..3231)
                     /locus_tag="AC16V7_14345"
     CDS             complement(2188..3231)
                     /locus_tag="AC16V7_14345"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6258350.1"
                     /translation="MRLNRVASWFWPAVPVVAALLFTAGLLFLFQASPIESFMAMYDG
                     AFGDQSKTLSVLAFWVPITLASSGLLVTFTAGLWNIGVEGQIILGSIAASWIALKFKA
                     PTPFIQVTLEILAAMAGGALWAALTAVLKTRGGVNEIFGGLALNNLAIILTNYLISGP
                     WQPPEGGTFRGTQPFAPEALLPRYAESRFSPISMVITFVVIGLLIVMLRGTFWGLKLK
                     ALGKNAYSSFLLGVSSEREVILAMMLCGALAGLGGAVRVLSWFDSLRQSISGGIGFLA
                     LLVVMLANYHAYWVPFIAIFFSAVLNGSITLQLRTQLHSSLGGILTGVMVLFVLLFGD
                     VGVLRWFRKEKQE"
     gene            complement(3228..4694)
                     /locus_tag="AC16V7_14350"
     CDS             complement(3228..4694)
                     /locus_tag="AC16V7_14350"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6258351.1"
                     /translation="MQIELKEIRKYFGNVKANDGISLVLQAGKIYGLLGENGAGKSTL
                     MKILSGYQPADSGSILLDGQSVHFTSPAQALSNGIGMLYQEPLDFPPFRIVDNYLLGR
                     DRKLMLDHKSAENILNNLASRYSFELDVHAFVDSLSMGERQQLELVRLLAGGAEVLIL
                     DEPTTGISAEQKDLLFDSMRKLAHEEGKTLILVSHKLDEVQELCDHAYVLRRGKLVGE
                     IDIPCPNEKLVEMMFAQIPPRTDRAANIIPGHPVVEMQDVTISTYRLSIEKINLAIQP
                     GEVFGLAGLEGSGQQLVLQACTGLLHPVHGHMRLAGQDVTNWSYHHMREAGVAYIAAG
                     RLEGGLVAGLSLTEHFILAQPEHTFFLDWSGAQKESASRIEKYQVVGTPLSLADELSG
                     GNQQRLLFALLRDDLKLVVMEHPTRGLDVRSSDWIWNILYERRKEGTAILFMSADLDE
                     IVERSDRIAVFSGGRMSRIVNAKETSVEELGHLIGGQE"
     gene            complement(4755..5717)
                     /gene="arcC"
                     /locus_tag="AC16V7_14355"
     CDS             complement(4755..5717)
                     /gene="arcC"
                     /locus_tag="AC16V7_14355"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HIR6258352.1"
                     /translation="MGNNGNKVAVVAIGGNSLIKDKNHQTVEDQYLAAKETTFHIADM
                     IEAGWDVAIGHGNGPQVGFILRRSEIAHKVEGMHEVPLDVCGADSQGAIGYALQQTLQ
                     NELYKRGIQKPVATVVTQVLVDKDDPAFRNPSKPIGGFMDEAEAQTRAKEMGWSVVED
                     AGRGWRRVVASPLPKEVVELESVNALIKHGVVVITVGGGGIPVIDAGDGEYRGTAAVI
                     DKDYASSLLAREINADLFLISTAVEKVGLNYGKPDQKWIDRMTLAEAKQYLADGTHFA
                     KGSMAPKVQAIIWYLEAGGKQAIITNPENIGRALRGETGTWIVP"
     gene            complement(5739..6719)
                     /locus_tag="AC16V7_14360"
     CDS             complement(5739..6719)
                     /locus_tag="AC16V7_14360"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005543 - phospholipid binding [Evidence
                     IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0006593 - L-ornithine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR6258353.1"
                     /translation="MQTDLRNRDLIGDLDFSREEVETVMEVAFDLKRKRALGESHAYL
                     RDKVLAMLFFFSSTRTRGSFEAGMAQLGGHAAFIESKTTQIAHGDTPKEIGEIFGRYF
                     DGIAIRHVDWGVGNGYLNAVAKASRVPVLNMQCDIYHPFQCLADLMTIFEKKGRDLRR
                     KRMVVSWAYAASYQKPMSVPQSLVLQMPRFGMDVILAHPPEFKLMPDIMDQAQEQARK
                     FGVGFEVMDDMEAAFKDADIIYAKSWGAMVHTPDPEEGGKIIKKYSHWITDERKMALA
                     KPDAIYMHPLPADRNIEVTDGVMDGPNSVVYDEAENRMHAQKAVMALTMR"
     gene            complement(6734..7684)
                     /gene="pyrB"
                     /locus_tag="AC16V7_14365"
     CDS             complement(6734..7684)
                     /gene="pyrB"
                     /locus_tag="AC16V7_14365"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HIR6258354.1"
                     /translation="MRSFNGRDILSLKGFEREEYFRVFEVADELSPFARNRRNTDLLK
                     EKTLVTAFYQPSTRTRLAHEAAMHRLGGHVTGFSDAKMTRAGDFYQESIKDTVKMLEF
                     YGDAIVMRHFQQGAPHEAARWASIPVINAGDGWGEHPTQVLTDLYTIQEEFGTLDGLN
                     FLLIGDMRMRTMHSISYAMTQFDINAFYVSPPEMSLTEDFKKELVDLNLNFKEIEHVE
                     QAIAEADVIYMEPVVQPDYTKSRDERTGEVGGTPANYKVTKELLRKAKQSAIVLHSLP
                     RMDELPTEVDDTRHERYWIEAFNGVVVRMALLALVLGAME"
     gene            complement(7762..>8419)
                     /locus_tag="AC16V7_14370"
     CDS             complement(7762..>8419)
                     /locus_tag="AC16V7_14370"
                     /inference="COORDINATES: protein motif:HMM:NF019322.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR6258355.1"
                     /translation="THNGGRLPSEPELARRLGVSRATLREAMRTFETQGLIRRRQGSG
                     TYVVHPTHVLESGLEMLESIETMAERIGLRVSMGELKIQHRPATAEEARNLALPASNM
                     VMQLTRVMKAEGRPAAYLIDIIPDDVLPISELEEQFTGSVLDLIIRRNEPELLTSRCE
                     INADTASAEVARSLGIQRGDVLLRFVAYLYDSSGRVVDYSISYFLPGYFRFHVVRRVG
                     "
BASE COUNT         2148 a         2408 c         1988 g         1875 t
ORIGIN      
        1 ccagacgaat gcctgatgcc aggatgccga caagcacatt catagttaat aggtcagaga
       61 tcatgatgct gccttcactt ctttgacctg cgcggcctct tcgcgtatcg cttcactaag
      121 ctgccggcgc tgcaaacggc gcctccaaag atcagtgctg accacaaaaa ccaccagcaa
      181 tccgtttagc gctgtgatca atgccgaagg cacctgcacg gcgcgctgca tggcattggc
      241 tcccacgatg agtgcaccga agaggaacga ggccgggatt gtacccagcg ggtgtaattg
      301 cccaaagagc gcagctacaa tcccgttaaa tccagcactg ccagtaaatc cggtggcaga
      361 gccatctgta aacatgcggt ggtggactcc aaatacctgt accgcgcctg ccagcccccc
      421 gagcgcccca ctcagcaaca aggcgagaac aacataccgc tgcacgtgaa tacccgcata
      481 gcgagacgca tggggattca gccctactgc gcggattcta tagcctgtaa tggtgcgcca
      541 gagaaatatg aaaatgagaa tcgccatgat cactgccaat gcagatccta agtgaaggcg
      601 cgtgggcact aaacgaggaa ggtcgaacaa tctggataac cgcgcagttt gagggatttt
      661 cgatgctgcc tcaagttgaa ccggatcaat catcgggccg cgtaatagga aattcatccc
      721 ttgtacagcg atttggttca tcatgattgt actcaggatt tcattgacat taaaatacgc
      781 tttcaacaca ccaggtatgc ccccccatat cgctccgctc aaaaagcccg ctaacagcgc
      841 caaggggatg ataagccagc ctggtgcttc ggtgaaagat aatcccacca gggtggaggc
      901 aatcgcccca accaccagtt ggccctcacc gccaatattg atcacgccgc ccctaaaagc
      961 gatacaaatg ccgataccca caagcagcaa gggtgtagct ttcaccaggg tatccgctag
     1021 agcattctgg ctgccaaagg ctccttccag cagcgcagag taggccacaa gagggttgac
     1081 gcccaacagg agtaacatca ccgcacccac agctagagct gccagagctg ccaggatggg
     1141 cataaagaaa ttagctagct tgtcccaacg cggattgata cctaaaagta atttcataaa
     1201 ataacgaatt atcctatcct ttttcagcca gagagctccc cagggctagc ggagcattga
     1261 tacgcaacat gcgcctcaga ggcggttgga tccgattagg catcacgcta actatccgct
     1321 cggtcacgtc actgctgact acgaggagaa ctgcgatcat caacacccag gggatagcat
     1381 tgaaagccac aaccgagacc tctgggaagg tgcgctgaag gatcgtagcc agtgcttttg
     1441 tggcaccgaa caagattgcg ccaagcgcac cgcgaatcgg agaccagccg ccaaaaataa
     1501 caatcgccag tgcaatccag cccaagccgc gggtatgccc atccgaccag cctattttaa
     1561 cgtctaacga ataggctgcg cctgctacac ccaccagcgc gccgccgacc agtgcataca
     1621 gatagcggac acgccgtaca ttcactccgc gagcgaaagc tgcctcaggt cgctcaccac
     1681 tgcttcttaa gtgtaacccg ggttgggtgc gattgagaaa ccaccaggtg aagataacca
     1741 acgcataggc aaaatacacg acaatatctt gtttgaataa gatcggtccg agaaaaggga
     1801 tatcttccaa aaaggggata ccaaaacgcg tgaccaatgc gcccgggatg cgagtgaagt
     1861 tctgcccgag aaatgcgctt aagtcatcac ctagaagtgt gagcacaaat ccaactgcca
     1921 cctggctctt tccgagagag atgctgctga atgccacaat cgcaccaaat attgccccaa
     1981 taattgctcc cgccaagaaa ccaagccaga cgttatcggt ttttagccca acaacaaatc
     2041 ccgtcattgc cgccatgagc atcgtgccat ccagggatag gtttaccaca ccggaccgct
     2101 ctgtaattgt ctcgccacaa acagcaataa tcaggggcga agaagctaaa atgatggagg
     2161 ccatcgtgcg gacgaattcg atctcattca ttcttgcttc tccttgcgaa accacctaag
     2221 tacaccgaca tccccaaata acaggacaaa caaaaccatc acaccggtca agataccacc
     2281 caaggatgag tgcaattgcg tacgcaattg caatgtgata ctgccattta agaccgcaga
     2341 gaaaaaaatc gcaataaagg gtacccaata ggcatgataa ttcgccaaca tcaccaccag
     2401 caaggccaaa aagccaatgc ctccggagat gctttggcga aggctgtcga accagctgag
     2461 cacacggaca gctccaccca agccggctaa tgccccgcac agcatcatcg ccaggatgac
     2521 ttcccgttca ctggacaccc ctagaaggaa tgaggagtag gcatttttac ccaatgcttt
     2581 gagcttcaaa ccccaaaacg tcccgcgcaa cataacaatc agcaaaccga tcaccacaaa
     2641 tgtgatgacc atcgaaatag ggctaaagcg tgattcagcg tatcggggta gcagggcctc
     2701 tggagcaaat ggctgtgtac cccgaaaagt acccccctcc ggtggttgcc aggggcctga
     2761 gatgaggtaa tttgtcagga taatcgccag attgttcaaa gccagcccac cgaagatctc
     2821 gttcactccc ccccgggttt ttaatactgc agtgagagcc gcccagagcg cccctcccgc
     2881 catggctgcc aggatttcca atgtcacctg gataaatggt gttggcgctt tgaatttcag
     2941 cgcaatccaa cttgctgcaa tcgagcccaa aatgatctgt ccttcgacac cgatattcca
     3001 caaacccgca gtgaaggtta ccaataaacc ggaagaggct aaggtaatcg gcacccaaaa
     3061 tgccagcaca ctcagggttt tcgactggtc gccaaatgcg ccatcataca tcgccataaa
     3121 tgattcaatg gggctggctt ggaataagaa cagcaagccc gcagtaaaga gcaatgcagc
     3181 cactacaggc acggcgggcc agaaccagct tgccactcga ttcaatctca ttcttgtccc
     3241 ccgatcaaat gtcccaattc ttccacggaa gtctcctttg cattcacaat gcgtgacatc
     3301 ctcccaccag agaagaccgc aatgcgatcg ctgcgctcca cgatctcatc cagatcggcg
     3361 gacataaaca atatggctgt tccttctttc cggcgttcat ataatatgtt ccaaatccaa
     3421 tctgaagaac gaacgtcaag cccgcgggtg ggatgctcca tgacaacaag ctttaaatca
     3481 tccctcaaca aggcaaacaa taagcgttgc tggttaccac ctgaaagttc atccgccaat
     3541 gagagcggag tacctaccac ttgatatttt tcaattcggg aagcagattc cttctgcgca
     3601 cccgaccagt ccaagaaaaa tgtgtgttca ggctgcgcta agatgaaatg ctcggttaac
     3661 gacagaccag caactaatcc cccttctaag cgaccagccg ctatataggc tacaccggct
     3721 tctcgcatat ggtgataaga ccaattcgtc acatcttgac cagcaagacg catatgccca
     3781 tgcacaggat gaagcagacc ggtacaggcc tggagcacaa gctgctgtcc actgccctcc
     3841 aagcccgcca atccaaacac ttcaccgggt tggatagcca gattgatctt ctcaatggat
     3901 aaacggtatg tggagatcgt gacatcctgc atctcaacaa ctggatgccc tgggatgata
     3961 tttgctgcac ggtcagtgcg cggagggatc tgggcaaaca tcatctcgac cagtttctca
     4021 tttgggcatg ggatatctat ctcgcccacc agcttgcccc gcctcaagac ataggcatga
     4081 tcacatagct cttggacttc atcaagcttg tgtgatacca gtatcagcgt cttaccttct
     4141 tcatgggcaa gctttcgcat tgagtcgaac agcaaatctt tctgttcggc gctgattccg
     4201 gtagtcggtt catctaaaat caagacctca gctccccccg ctaagaggcg caccaattca
     4261 agctgctgtc gctctcccat ggagaggcta tctacaaatg catgcacatc caattcaaag
     4321 ctgtagcgtg atgcaaggtt gtttaaaata ttttctgcgg atttatgatc cagcataagc
     4381 ttgcggtcac gacccaggag ataattatcc acgatgcgga aaggaggaaa atctagcggc
     4441 tcctggtaga gcatgcctat accattggat aaagcctggg ctggcgaggt aaaatgcaca
     4501 ctctgaccat ccaataagat tgatccacta tcagccggct ggtagccaga taaaatcttc
     4561 atcaaagtgc tcttaccagc accattttcc cccaataacc cataaatttt acctgcctga
     4621 aggacaagac tgataccatc atttgccttg acattcccga agtatttgcg gatctctttg
     4681 agttcgattt gcatcgcgcc agccctccag gcagggtttt agttgcagat attttatcct
     4741 atgatttaat aaatctaggg cacaatccac gtgcctgttt cacctcttaa tgctcgcccg
     4801 atattctcgg gatttgtgat gatggcttgc ttgccgccgg cctctaggta ccagatgatc
     4861 gcctgtacct tgggcgccat ggagcctttg gcaaagtggg tgccatcagc cagatactgc
     4921 ttagcctcag ccagtgtcat gcggtcaatc catttctgat ccggcttccc gtagttgaga
     4981 cctactttct caaccgcggt ggagatgagg aaaaggtcgg cgttgatctc gcgtgccagc
     5041 aagctgctgg cgtagtcttt atcgatcact gcggctgttc cacgatattc gccatcaccg
     5101 gcatcgatca caggtatgcc tcctccaccc acagtaatca caaccacacc gtgcttgatg
     5161 agtgcattga ccgattccag ctcgaccact tcctttggta atggcgacgc aactacgcgc
     5221 cgccagccac gaccagcatc ttccacaacg ctccacccca tctccttggc gcgcgtttgg
     5281 gcttcagcct catccatgaa tccaccgatg ggcttggatg ggttgcgaaa ggccggatca
     5341 tccttatcca ccagcacttg ggtgaccacc gtagccactg gtttttggat accgcgcttg
     5401 tacaactcgt tctgcagggt ctgttgcaac gcatatccga tggcgccctg gctatctgca
     5461 ccgcatacat ccagcggcac ctcgtgcatg ccctcaacct tatgagctat ttccgagcgg
     5521 cgcaggatga agcccacctg cggcccgttg ccatgaccga tggcaacatc ccaacctgct
     5581 tcaatcatgt cggcgatgtg gaaagtggtc tccttagccg ccaagtattg gtcttccacc
     5641 gtctggtggt ttttatcttt aatgagcgaa ttgccaccga tggcaactac ggccacttta
     5701 tttccattgt ttcccatgtc gctgttcctc ctgcctaact atctcattgt cagcgccatc
     5761 accgctttct gcgcgtgcat gcggttttcg gcttcgtcat aaactacgga gtttggacca
     5821 tccattacgc catcggtgac ctcaatattg cgatcagcag gcagcggatg catataaata
     5881 gcgtctggct tagccagagc catttttcgc tcatctgtaa tccagtgcga atacttttta
     5941 atgatcttgc cgccctcttc cgggtcggga gtgtgaacca tagcacccca agatttagcg
     6001 tagatgatgt cggcatcctt gaaggcagct tccatatcat ccatgacctc aaaaccgacg
     6061 ccgaacttgc gcgcctgctc ttgagcctga tccatgatat ccggcatcaa tttgaactcc
     6121 gggggatgag ccaggataac atccatgccg aagcgaggca tctgcaacac cagtgactgc
     6181 ggcaccgaca tgggtttctg atacgaagca gcatacgccc acgatactac catgcgcttg
     6241 cggcgcagat cgcggccttt tttctcaaat atcgtcatca agtcggctaa gcattggaat
     6301 ggatgataaa tatcgcactg catgttgagc actggcacgc gtgaggcctt agccaccgca
     6361 ttcaggtaac cattgccaac cccccaatcc acatggcgga tagcgatgcc atcaaaatac
     6421 cgcccaaaga tctcgccgat ctcctttggg gtatcgccgt gagcaatctg ggtggtcttg
     6481 ctctcgataa acgcggcatg accgccgagc tgagccatgc cagcttcaaa cgagccacgc
     6541 gtgcgcgtgc tggagaagaa gaacaacatg gccagcactt tgtcgcgcag gtaagcgtgc
     6601 gattcgccca acgcgcgctt gcgcttcagg tcgaaagcca cttccataac tgtttcaacc
     6661 tcttcgcggc tgaaatccag atcgccaatc agatcacgat ttcttaagtc tgtttgcatc
     6721 ttacacttcc tgtttattcc atcgcgccga gaaccaacgc cagcagtgcc atgcgcacca
     6781 ccacgccatt aaacgcctcg atccaataac gttcgtgacg ggtgtcgtcc acttcggttg
     6841 gaagctcgtc catgcggggc aaggaatgta acacaatggc actctgcttt gctttgcgca
     6901 ataactcttt tgtgaccttg tagttcgccg gggtcccacc cacttcgccg gtgcgctcat
     6961 cacgcgactt ggtgtaatct ggctgtacaa ccggttccat gtaaatcacg tcagcttccg
     7021 cgatagcctg ttcaacatgt tcaatctctt tgaagttcag gttgaggtcg accagctcct
     7081 tcttgaaatc ttcagtcaga ctcatctctg gaggggagac atagaaagcg ttgatgtcaa
     7141 actgggtcat agcgtacgaa atgctgtgca tagtgcgcat gcgcatgtcg ccgatgagca
     7201 agaaattaag cccgtccagc gtgccaaact cttcctggat ggtgtacagg tcggtcagca
     7261 cctgggtggg atgctctccc cagccgtccc ccgcgttgat caccggaatg ctggcccagc
     7321 gtgcagcttc gtgcggcgcg ccttgctgaa agtggcgcat cacgatggca tcgccgtaaa
     7381 attccagcat ctttacggta tctttgatcg actcctggta gaaatcacca gcgcgggtca
     7441 tttttgcatc agagaaaccc gtcacatgac caccaaggcg atgcatggcg gcttcatgtg
     7501 ccagccgcgt gcgcgtgctg ggctggtaga aagccgtcac cagtgttttt tccttgagca
     7561 gatcagtatt acgccgattt cgagcaaatg gagacagctc gtcggctact tcgaagacgc
     7621 ggaaatattc ctctctttcg aagcctttga gtgacagaat gtcacgtcca ttgaaactac
     7681 gcatcttact acctccgaga actcattctt aaaattgaga ttcgttgcgc atgatacatc
     7741 gctgtgcgtc atgccacacg gttaacccac ccggcgtaca acatggaatc ggaaatatcc
     7801 gggtaggaaa taactaatag aataatctac aacgcgacca gacgagtcgt acaaataagc
     7861 cacaaagcgc aggagcacat ctccacgctg aataccgaga gaacgcgcca cctctgctga
     7921 ggctgtatcg gcgttgatct cgcatcgtga tgtgagcaac tccggttcgt tgcggcgaat
     7981 gattagatca agcaccgagc cagtaaactg ttcttcaagt tcagagatcg ggagaacatc
     8041 atctgggata atatcaatca gatatgcagc aggcctacct tcggctttca tgacccgggt
     8101 cagttgcatc accatattgc ttgccggcaa ggccaggtta cgtgcctctt cagctgttgc
     8161 cgggcgatgc tgaatcttta actcccccat ggaaacccgt aaaccgatgc gctccgccat
     8221 cgtctcgata gattccagca tctccaaccc gctctcaagc acatgggtag gatgcacaac
     8281 ataggtacca gaaccctgac gacggcggat caatccctgg gtttcaaagg tgcgcatggc
     8341 ctcgcgaaga gtagccctgg aaacacccaa gcgccttgct agttcgggct ccgaagggag
     8401 tctgccaccg ttatgggtg
//
LOCUS       DBNHOH010000799         2120 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1652889, whole genome shotgun sequence.
ACCESSION   DBNHOH010000799 DBNHOH010000000
VERSION     DBNHOH010000799.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1652889"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1313)
                     /gene="gntE"
                     /locus_tag="AC16V7_14375"
     CDS             complement(3..1313)
                     /gene="gntE"
                     /locus_tag="AC16V7_14375"
                     /inference="COORDINATES: protein motif:HMM:NF041362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanitoxin biosynthesis PLP-dependent
                     transaminase GntE"
                     /protein_id="HIR6258356.1"
                     /translation="MGLQGKNTKASYEKAKKLIPHGVNSNFRFWGDDDTLIVSHGEGA
                     YVWDLDGKRYIDYRLGFGPIILGHGYPAVVERVGEAIKGGVTFAWTTSLEIQVCERLT
                     RMCHVDKVRLTNTGSEATMHALRIARAYTGREKFIKFEGHYHGQCDYFMFSTASSSPA
                     ALGSRRAPIRAPVTSGIPTGINQYVIVLPFNDLERLEETIEAQWGDLAAVFVEPLMGN
                     CAGIVGKPEFIKKLRELCDKFGIMLVFDEVKTGFRIAKGGAQEFYDVRADLVTYAKAM
                     GNGFPIAAIAGKENVMMVIEPGAMAHGGTYSGNVVGTAAADATLEILENEPVFETIQQ
                     RGKALMKGIDDILAEAGLPHVVTGVPSMFGISLGSAKAPQEYRDYHKGDTELYEKISL
                     ELNRRGVQPDSDGREPWFLCYALSNEDVGETLNIFNDSVKAAMR"
     gene            complement(1427..>2120)
                     /locus_tag="AC16V7_14380"
     CDS             complement(1427..>2120)
                     /locus_tag="AC16V7_14380"
                     /inference="COORDINATES: protein motif:HMM:NF018012.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR6258357.1"
                     /translation="IQGNCEVLSMLCLVQAATPRTPVIYAPVLAAMDPHTGLYSGGAI
                     ENGILGVAAIEMGRYYGLPVEGVGGGTSQFIPGIQAGYERVLTGFMPTLKLPDLIVGP
                     GLVGNSMVLSLEQLVIDVEIFRMSMHAYRGIDTREGNWLNDVIQQVGPGGHYLSERST
                     VQAFRNGEMYLPNLGYRGPYLAWKEAGGTTLLQEAHEKVEYLLANYDPLPLSEEVERE
                     LELLQKRVEFTI"
BASE COUNT          522 a          628 c          450 g          520 t
ORIGIN      
        1 aatcaccgca tggcagcttt tactgagtcg ttgaatatat tcaaggtctc accaacatct
       61 tcattggaca gcgcgtaaca caggaaccag ggctctcgcc catcagaatc gggttgtacc
      121 ccgcgccggt tcaactccag gctaattttc tcataaagct cagtatcccc tttatggtag
      181 tctctgtact cttgcggcgc ctttgcgctc cctagagata tcccgaacat agagggcacc
      241 ccagtgacga catggggaag ccctgcttca gcaaggatat cgtcaatgcc tttcatcagt
      301 gctttgccac gttgctggat ggtttcgaac acaggctcat tctctaagat ctcgagtgtg
      361 gcatctgctg ctgcggtgcc aactacgttg ccagaataag tgccgccatg tgccatcgcc
      421 ccaggttcga tcaccatcat gacattttcc ttgccagcta ttgccgcaat tgggaagcca
      481 tttcccatgg ctttggcata tgtgaccagg tccgcccgga cgtcataaaa ctcttgggcg
      541 cccccctttg caatcctgaa accagttttt acctcgtcaa aaacaagcat gataccgaat
      601 ttgtcacaaa gctcgcggag cttcttgatg aactcgggct tcccaacaat accagcacaa
      661 ttgcccatca atggctcgac aaagacagcc gccaggtcac cccactgggc ctcaatggtc
      721 tcctccaacc tttccagatc attgaacggc agaacgatca catactggtt aatccccgtt
      781 gggatacctg aggtaaccgg cgcgcggatg ggcgcccggc gcgaacccag ggctgctgga
      841 gaggaagaag ctgtgctaaa cataaaatag tcacattgac catgatagtg gccctcaaat
      901 tttatgaatt tttcccgtcc tgtgtacgct cgagcaattc tcaaagcgtg catagtagcc
      961 tcggaacccg tatttgtcag gcgaaccttg tccacatggc acatcctcgt gaggcgctcg
     1021 cacacttgta tttccaggga ggttgtccag gcaaatgtca cgcctccttt gatggcctct
     1081 cccacccgct cgactacagc cgggtaccca tgccccaaaa ttatgggtcc aaagcccaat
     1141 cggtaatcaa tgtacctttt gccgtctaaa tcccaaacat atgcaccctc cccgtgcgag
     1201 acaattagtg tatcgtcgtc tccccagaac cggaaattcg agtttacgcc gtgcggaata
     1261 agtttctttg ctttctcata cgaagccttt gtattttttc cctgtagacc catggtgata
     1321 tcctccacct aaaataatag tttgtatgta ctcttcccac cagaaatcgc tagcgagagg
     1381 taatcttcgt cataatcagc tgccaatcca tttcctcaac ataactttaa atggtgaact
     1441 ctaccctctt ttgtagcagc tctaattcgc gctccacctc ctccgacagg ggtaatggat
     1501 cataattggc aagcaagtat tcaacttttt catgcgcttc ttgtaacagt gtagttccac
     1561 cggcctcttt ccacgccagg tatggaccac gatagccaag atttggcagg tacatctcac
     1621 cattccgaaa tgcttggacg gttgaacgtt cactcaaata gtgcccacca ggcccaacct
     1681 gctgaatgac atcattgagc caatttcctt cacgggtgtc aataccccga taagcatgca
     1741 tactcatccg gaaaatctca acatctatga ccagctgctc caggctcaac accatagaat
     1801 tgccgaccaa tcccggacct acgatcaagt ctggcaactt caaggtgggc ataaatccgg
     1861 tcaagactcg ttcatagccc gcctgaatcc caggtatgaa ctgggacgtt cctcccccta
     1921 ccccttcgac cggcagacca taataacgac ccatctcaat tgcagccacg cccaaaatgc
     1981 cattctcaat cgcccctcca ctatataaac ccgtatgggg atccatcgcg gcgagtaccg
     2041 gtgcatagat aaccggcgta cgcggtgtcg ctgcctggac caggcaaagc atggaaagaa
     2101 cttcgcaatt cccctggatg
//
LOCUS       DBNHOH010000800         1966 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_435080, whole genome shotgun sequence.
ACCESSION   DBNHOH010000800 DBNHOH010000000
VERSION     DBNHOH010000800.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_435080"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..953)
                     /locus_tag="AC16V7_14385"
     CDS             complement(51..953)
                     /locus_tag="AC16V7_14385"
                     /inference="COORDINATES: protein motif:HMM:NF013648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HIR6258358.1"
                     /translation="MDNHPVDKENGLWLSRHFENPPKRVVSLVPSLTESLFELGFGEV
                     VVGVTDYCVHPSEHLVDLPRLGGPKNPRVDEIIALQPELILVNWEENTRRTVEELEAA
                     GILVWVTFPKTVREALDLLWILAGVFHSRLAAARVESLEVALDWAQQAAQQRAPLRYF
                     CPIWYEKTSAGLPWWMTFNRHTYPHDLLLTLGGENVFSDRERRYPLEADLGLAEPQDS
                     DGWDTRYPRLSLEEIRLSNPELILLPNEPYAFTNNDRDELIAQLADTQAVQGGRVYPI
                     DGSLITWHGTRLARALQELPFLFE"
     gene            complement(1036..>1966)
                     /locus_tag="AC16V7_14390"
     CDS             complement(1036..>1966)
                     /locus_tag="AC16V7_14390"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20 metallopeptidase family protein"
                     /protein_id="HIR6258359.1"
                     /translation="IVEESEVEYASQNLGVMHACGHDGHTAVGLTVARLLQAHSQELA
                     GTVKFVFQPAEEGLGGALAMVADGALEIPRPDFALALHLWNEKPIGWLGISPGPVMAA
                     SEIFKVRVIGKGGHGASPHLTVDPVLAGAHIVTALQGIVARNVPPLESAVVSVTTFRS
                     GESFNVIPQVVEMMGTIRTFVPSVRQLVLERFRQVVEGVAAAMDCRVEVEIASITPAV
                     VNDLSLTQGVQETARRLWPNNPLDLDYRSMVSEDMAFMMQDIPGCFMFIGTANVEKGL
                     NAPHHHPSFNIDEEALPRAAALMSAAVAAHMGA"
BASE COUNT          490 a          579 c          456 g          441 t
ORIGIN      
        1 ctttctgagt gtcaaaataa ggttaaattc cttatctcaa tttgtctggg ctattcgaaa
       61 agaaagggga gctcttggag tgccctagcc agccgtgtac catgccaggt gatcaagctg
      121 ccgtctatag gatatactct cccgccttgc actgcctggg tatcagccaa ttgggcgatt
      181 aattcatcac ggtcgttgtt tgtaaaagcg tatggctcat taggaagcaa gatcagctca
      241 ggattggaaa gacggatctc ttccaaactt agcctcggat accgcgtgtc ccacccgtca
      301 gaatcttgtg gttcagctaa gcccaagtct gcctcgagag ggtagcgccg ttcacggtcc
      361 gaaaaaacat tttctccccc aagtgtcaga agcaagtcat gtggataggt atggcgattg
      421 aatgtcatcc accatggcaa gcctgcgctt gtcttttcgt accagatggg acaaaaatag
      481 cgaagaggtg ctcgctgttg ggcggcttgc tgggcccaat ccagagcgac ttccaggctt
      541 tccacccgtg cagccgccaa tctgctgtga aacacgcccg ccagtatcca aagtaaatcc
      601 aatgcctcgc ggaccgtctt aggaaacgtc acccaaacca ggatgccagc cgcttccagc
      661 tcttcgaccg tccggcgagt gttctcctcc cagttcacaa ggattaattc aggctgcaac
      721 gcgataatct cgtccactcg ggggtttttt ggaccaccca agcgtggcag atctaccaaa
      781 tgctcagatg ggtgcacaca gtaatccgta acacccacta ccacctcgcc aaagcccagc
      841 tcgaacaggc tttccgtcaa agatggcact aatgaaacta ctctcttcgg agggttttca
      901 aaatgcctgc tgagccaaag gccattttcc ttatcaacag gatgattgtc catgtctcca
      961 acagtgttta tcaccacaac taaccatata cgctgcttgt ctaaagtcct tttgcctgag
     1021 ggcttgacac aagcttcatg ctcccatatg agctgcaaca gccgcggaca tcaacgctgc
     1081 agcgcgtgga agtgcttcct cgtcaatatt gaaactcggg tggtggtgag gcgcgttcaa
     1141 gcctttttcg acattggcag ttccgataaa catgaaacaa ccaggaatat cttgcatcat
     1201 gaaagccata tcctccgaga ccatcgatct atagtcgaga tccaaggggt tatttggcca
     1261 caatcggcga gcagtttctt ggacaccctg agttaaactc aaatcgttga ccaccgccgg
     1321 cgtaatcgac gcgatctcca cttcaacccg gcaatccatt gcagcagcta caccttccac
     1381 cacctgccgg aaacgttcca gcaccagctg gcgcaccgaa ggcacaaacg tgcggattgt
     1441 ccccatcatc tccacgacct gcggaattac gttgaatgac tctccgctac gaaaagtagt
     1501 tacactgacc acagccgact caagcggcgg gacattgcga gcgacgatcc cctgtaatgc
     1561 ggtgacgata tgcgccccag ccaacacagg gtcaactgtc aggtgaggtg aagccccatg
     1621 cccacctttt ccaatgaccc gcactttgaa aatttctgaa gcagccatca ccggaccagg
     1681 gctaatcccc agccagccga tgggcttctc gttccaaagg tgcaacgcta gagcgaaatc
     1741 aggtctcgga atctccagcg cgccatccgc gaccattgcc agagccccac ccaacccctc
     1801 ctcggcgggt tgaaacacga atttgacagt acctgccaac tcctggctgt gggcttgcag
     1861 cagacgcgcc acggttagac caacagcagt atgcccatca tgaccacaag catgcataac
     1921 acccaagttt tgtgaggcgt attccacttc gctctcttca acgatt
//
LOCUS       DBNHOH010000801         5923 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1525185, whole genome shotgun sequence.
ACCESSION   DBNHOH010000801 DBNHOH010000000
VERSION     DBNHOH010000801.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5923)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5923
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1525185"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..1823
                     /locus_tag="AC16V7_14395"
     CDS             303..1823
                     /locus_tag="AC16V7_14395"
                     /inference="COORDINATES: protein motif:HMM:NF014565.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004576 - oligosaccharyl transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STT3 domain-containing protein"
                     /protein_id="HIR6258360.1"
                     /translation="MSRANRLPLLLSLLAILAAYLVAERVFERMPHIEDEIAYLWQAQ
                     AIAGGHLTVPTPAHPKSFLVPFVVDYNGQRFGKYPLGWPAALAVGVFFGARQWVNPFL
                     AGLGVWLTYLLAKRLFGQTVGLLAALLTLTSPFFLMNSGSLLSHPFGLVLSTTFALAW
                     LDSWDHESMRPWLATIAAALALGLLAFTRPLTALGVSLPFLPHAAILWFREGWSTRRR
                     LLVFALIVLGVSSLIFVWQWAVTGDPLFNPYTLWWDYDKVGFGPGVGRLEVGHSLHQA
                     RINTEHSLWVGWHDYFGWGRFSWIFLPFGALALLRARLAKALPVFLVFPSLVVIYLAY
                     WIGSSLFGPRYFYEGLYSLTIASAAGIAFLAGWPIHPGEPWTDRLGWARARPLLITAL
                     VALLLSANLLFYTPIRLGGMHGLYGISRDRLAPFQSTQAQGMIPALVIVHPDRWTEYG
                     SLLELTDPFLNTPFLVVISVGDNADAALAADYPDRAVYHYYPDRPWVFYKRPLNSP"
     gene            complement(1896..2936)
                     /locus_tag="AC16V7_14400"
     CDS             complement(1896..2936)
                     /locus_tag="AC16V7_14400"
                     /inference="COORDINATES: protein motif:HMM:NF013200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR6258361.1"
                     /translation="MSNKVFVYIDQFKGAALPVSWESLSAARSLAEQLGGGVTALVIG
                     PQSQGVANEAFQYGADEVLYADDATLADYRPEPYTALISKLVKDAATEALLFPTTSRG
                     RELAAMSAVDLESGVMPDVTALSVEGGQVIATRPVYAGKLLSRVVCSAKPQIATTRTR
                     AFPAPQPDASRSGTPTKVDAVLAEADISSKVVEYTTSDGGVSLADAAVIVSGGRGVSN
                     NPALTPPAGLDEKQAEVWRAQQGFKLVSDLATVLGGAVGASRAAVDAGYIPYEHQVGQ
                     TGKVVSPDLYIACGISGAIQHQAGMRTSKMIVAINKDPEAPIFKMARFGVVGDLYAIL
                     PPFTEALRKKLG"
     gene            complement(2936..3715)
                     /locus_tag="AC16V7_14405"
     CDS             complement(2936..3715)
                     /locus_tag="AC16V7_14405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR6258362.1"
                     /translation="MKIVVCVKQVPDSAAKVVVENGTVTWGDAPLVINPWDEYAVETA
                     LLQKEALGGEVTAITLGGEGAKEALKHALAMGASDAILISDPTMSESDSQSVARVLAA
                     AIQKLGDVKVAFFGRQAIDGDMGVTAAQTARALGWPMLSLVSVINSLDPAAGTIRVGR
                     ATEEGRQVVEAKLPAVVSVGKDIAEPRYPSFMGIRKASRAEIPTWSLAELGISAPSAV
                     VKWPEVMNPPTREVVTELVSGGSPQEVAENLAEKILAEKVL"
     gene            3828..5300
                     /locus_tag="AC16V7_14410"
     CDS             3828..5300
                     /locus_tag="AC16V7_14410"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6258363.1"
                     /translation="MPWAIIHPVRRNTEVSILARVLLPLVLLALLGIAPRPHLVTRAL
                     WSANLAIRQDVSPQFAARSARALAQAAEQIPWRSDLWEPAGRLALQGKEPLAGITYFE
                     RAASQNALSLDGQITLGDSYAETGDRAAAIDAWESALSSGSPSTEVLTRLLDAHLSLG
                     DYPAAVVDLQGLTALHPADADLRFQLGLLLSTLQPEAALAHLAQAAELDPELDAKAQK
                     LIASIRTGSLAGDPAQTLFEAGRALGSIGEWELAAQAFRQATLVRPDFTDAWAFWGEA
                     RQQLQEDFISQPVPEELERALELDPSSLSANVLMALYYQRRARFDQALEYLRKATELY
                     PQNPALYVELGEVLALSGDLEAALQAYQKTVELAPNDPAYYRLLAGFSARYEYQVGAV
                     GLSAARRAVLLDPANPDSLDSMGQVLFLLGDLTSAERFFLRALGADPNHAPAHLHLGL
                     AYMLQGERDRAYQEWKLVTSLASGTPAAEQAERLVRNYFP"
     gene            complement(5449..>5923)
                     /locus_tag="AC16V7_14415"
     CDS             complement(5449..>5923)
                     /locus_tag="AC16V7_14415"
                     /inference="COORDINATES: protein motif:HMM:NF037564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3048 C-terminal domain-containing protein"
                     /protein_id="HIR6258364.1"
                     /translation="NASNLWVFYAYLNQILWKYDPASGAYLRNPDKADGSGKFYPSTD
                     RLTGEQLAFENVIVLYAKHNVLNGARTLIDLDLFYTTNRAYLFRDGKVYPIYWSTVSG
                     DYEKKSGLLRPIRFVDGEGNPFPLKPGKTWVEIVDVTATMVEKEPGYWKARFYAP"
BASE COUNT         1055 a         1752 c         1713 g         1403 t
ORIGIN      
        1 gtctataagg gggtaatggc tggaacatac aaagcccccc atctcaaatt gctcagtcca
       61 accaatagag tgcttgaatc gctaaagcta ataggcttag acctgtatat cgatatcttt
      121 gataacaaac aggaagcttt agcggctttt taacttccca aaatactttc actgggctcc
      181 gccctcaact ttccgaactt tcaccccaac tacctcccgc tccgaggtgg taggtctcgc
      241 gaccttcgat ctcattattc gcccgaatta atggtacgat agattgaacg gtttgctcct
      301 tcatgtctcg cgccaatcgc ctcccccttc tacttagcct gctggcaatc ctggctgctt
      361 acttagtcgc cgagcgcgtc tttgaacgaa tgccccatat tgaagatgag atcgcctatc
      421 tctggcaagc gcaggcgatc gcaggcgggc acctgaccgt acctacgccc gcccacccga
      481 agagcttcct ggtgccattc gtcgtggatt acaatgggca acgctttggt aagtacccgt
      541 tgggttggcc agccgccctg gcggtgggtg tgttctttgg cgccaggcaa tgggttaatc
      601 cctttctggc tggactgggt gtatggctga catatctcct ggctaagcgc ctgtttggcc
      661 agactgtggg cttgttagcg gcgttgctga ccctgacttc cccattcttc ctgatgaact
      721 ccggctcgtt gctctctcat ccctttggat tggttttgag cacaaccttc gctttggctt
      781 ggctggactc ttgggatcac gaatcaatgc gtccctggct ggcaacaatc gccgcggctc
      841 tggccttagg cttgctagct tttacccgcc cattgactgc gctgggggta tcgctgcctt
      901 tcttgcctca tgccgcgatc ctgtggtttc gcgaggggtg gtcgacacgt cgccgcctgt
      961 tagtcttcgc tttgatcgtc ttgggtgtgt cgtccctgat ttttgtctgg caatgggctg
     1021 tgaccggtga tccgttgttt aatccttata ccctgtggtg ggattatgat aaggtcggtt
     1081 ttggaccggg ggtggggcgc cttgaagtag ggcactcttt gcaccaggcg cgcatcaaca
     1141 ccgagcacag cctgtgggtg ggttggcacg attactttgg ctgggggcgt ttttcttgga
     1201 tttttctgcc ctttggcgcc ctggcactcc tgcgcgcccg cttggcgaaa gccttgccgg
     1261 tgttcctggt ctttcccagc ctggtggtca tttacctggc gtattggatc ggctcttccc
     1321 tgtttggtcc ccgttatttc tatgaaggcc tgtatagcct aaccatagcc tccgcggctg
     1381 ggatcgcctt tctggctggc tggcccattc accctggtga gccctggacg gatcggctag
     1441 gttgggcgcg cgctcgcccg ctgctcatca ctgccctggt tgctctgttg ctgagtgcca
     1501 acctgctctt ctacaccccc atccgcctgg ggggtatgca cggattatat ggtatctcgc
     1561 gtgaccgctt agcgcccttt caatctaccc aggcgcaggg gatgatccct gcgcttgtca
     1621 ttgtccaccc tgatcgctgg acggaatacg gtagcctgct ggaattgacg gatcccttcc
     1681 tgaacacccc ctttctggtg gtcatcagtg taggcgataa cgctgatgcc gccctggcgg
     1741 cggattatcc agaccgggcg gtgtatcact attatcccga taggccgtgg gtgttttata
     1801 agcggccatt aaatagccca taagatcaac ctcccgcggg ttgaaccctg cgggaggttt
     1861 tttatctatt cattaagagg aagggacctg ccctgctagc cgagcttctt acggagagcc
     1921 tcggtaaatg gcggcaggat ggcgtacagg tcgcctacca cgccaaaacg agccatctta
     1981 aagatcgggg cctcggggtc cttgttaatg gctacgatca tcttgctggt gcgcatgccg
     2041 gcctggtgct ggatcgcccc agagattccg caggcgatgt acagatcggg ggagaccacc
     2101 ttgcccgtct ggccaacctg gtgctcgtaa ggaatatagc cggcatcgac ggcggcgcgg
     2161 ctggcgccca ccgccccgcc tagcacggta gccaggtcgg agaccagctt gaagccctgt
     2221 tgggcgcgcc agacctcggc ctgtttttcg tccagcccgg cgggtggggt gagcgctgga
     2281 ttattggaga cgccgcgccc gccagagacg atgaccgccg cgtcggccag gctcacaccg
     2341 ccatctgatg tggtatattc cacgactttg ctggaaatat cagcctctgc caggactgca
     2401 tcgacctttg tcggcgtacc tgaacgggag gcatccggct ggggtgctgg gaaggcacgc
     2461 gtgcgcgtgg tggcgatctg tggcttggca ctgcagacta ccctgctgag cagcttgccc
     2521 gcgtacaccg ggcgagttgc gatcacttga ccgccctcca cgctcagggc ggtcacatct
     2581 ggcattacac cggactcaag atctacagcg ctcatggctg ccagctcgcg gccgcggctg
     2641 gtggtgggaa agagcagagc ttccgtagcc gcatctttaa ccaatttgct gatcagcgcc
     2701 gtgtagggct cggggcggta atctgccagg gtggcatcgt cggcatacag cacctcatca
     2761 gccccatatt gaaaagcctc gttggccaca ccctgcgact ggggtccaat gaccagggcg
     2821 gtgactccgc cgcccagttg ctctgctaaa ctgcgggcag cacttaggga ttcccaggaa
     2881 acgggcaatg ccgcgccctt gaattgatca atatagacaa aaactttatt gctcatcaca
     2941 gcaccttctc cgccaggatt ttttcggcca gattttcggc gacctcttgc gggcttccgc
     3001 ctgagaccaa ctctgtgacc acctcgcggg tcggcgggtt catcacttcg ggccacttga
     3061 cgaccgctga cggggcagat atgcccagtt cggctagcga ccaggtgggg atctcggcgc
     3121 gtgaagcttt acggataccc atgaaggaag ggtaacgtgg ctcggcgatg tctttaccca
     3181 cgctaactac cgccggcaat ttcgcctcaa ccacttgccg tccctcctcg gtggcgcgac
     3241 ctacgcggat cgtgccagcc gccgggtcca gcgagttaat cactgacacc agggagagca
     3301 taggccatcc cagggcgcgc gccgtctgtg cggcggtcac acccatatca ccgtcaattg
     3361 cttgccgtcc aaagaatgca actttgacgt cgcctagctt ttggatggct gctgccagga
     3421 cgcgcgccac actctggcta tcactctcgc tcatggtagg gtcggagatc aagatggcat
     3481 cactggcgcc catcgccagg gcgtgtttga gagcctcctt ggcgccctcc cctcccaggg
     3541 tgatggcggt aacctcgccg ccaagagcct ctttttgtag aagagcagtc tcgacggcat
     3601 attcgtccca ggggttgatc accagcggtg catcacccca ggtaaccgta ccgttttcca
     3661 cgaccacttt cgccgccgaa tccggtactt gtttgacaca aacaacgatc ttcaacgtaa
     3721 cctcctttat cggtgagtta tatcgactca agttccatga gttggttggt cttggtttca
     3781 cacctgatta tagccaatcc aatagaagag gacaactgga ttttgtcatg ccttgggcta
     3841 taatccatcc tgtgagacgt aatacagaag tcagcatcct ggcacgagtg ctcctacccc
     3901 tggtcttgct ggccttattg ggaatagcgc cgcgcccgca ccttgtgacc cgcgcccttt
     3961 ggagtgcgaa tctcgctatc cgccaggatg tctcacccca gtttgctgcc cggtctgcac
     4021 gcgcgctggc ccaggcggct gagcaaatcc cctggcgctc cgacttgtgg gagcctgccg
     4081 ggcgtctggc tctgcaggga aaagagccgc ttgctggcat tacctatttt gaacgagcag
     4141 catcccaaaa tgctctttcg ctggatggtc aaatcacctt gggtgactcc tatgctgaga
     4201 ctggcgatcg tgccgcggcc atcgacgctt gggagagcgc tttgtccagc gggagtcctt
     4261 caaccgaggt gctcacccgc cttcttgacg ctcacctctc cctgggcgat taccccgcgg
     4321 ccgtggtcga cctgcaaggt ctgacagcct tgcaccctgc ggatgccgac ctccgtttcc
     4381 agctgggttt gctgctgtcc acgctgcagc ccgaagctgc tttggcgcac ctggctcagg
     4441 cggctgagct ggaccccgag ctggacgcaa aagctcaaaa actgatcgcc agcatccgca
     4501 cgggtagcct ggctggcgac ccggcacaaa ccctgttcga ggctggtcgc gccctgggtt
     4561 ccattggtga gtgggagctg gcggcgcagg ccttccgcca ggcgaccctc gttcgcccag
     4621 actttaccga tgcctgggcg ttttggggtg aagcccgcca gcaacttcag gaggatttta
     4681 tcagccagcc tgtgcccgaa gagctggagc gggcgttaga gctggaccca tcttcgctga
     4741 gcgccaatgt gctgatggct ctttactacc agcgacgggc gcgttttgac caggcattgg
     4801 agtatctgcg aaaggctacg gagctctatc ctcaaaatcc tgccctgtat gtggagctgg
     4861 gagaggtgct ggcattgagt ggagatctgg aggccgccct gcaggcttat caaaagaccg
     4921 ttgagctggc gcccaatgac ccggcgtact accgcttgct ggcgggtttc agcgcgcgct
     4981 atgagtacca ggtgggtgct gttggcctgt ctgctgcccg ccgggcggtg ctgctcgacc
     5041 ctgccaatcc cgactcgctg gacagcatgg ggcaggtgct tttcctgctg ggcgatctca
     5101 ccagcgccga gcgttttttc ctgcgcgccc tgggtgccga cccaaaccac gcccctgcgc
     5161 acctgcacct gggactggcc tatatgctgc aaggcgagcg cgaccgcgcc taccaggaat
     5221 ggaaactagt gacttccctg gcctctggca cgcctgccgc tgagcaggcg gagagattgg
     5281 tgagaaacta ttttccgtga ggtttagtga attttggggt ggagcctatt agcatcatca
     5341 cttcggttgg tgaattcctg cgccgctcgg tgatggttgt gtctaacaaa tcacaagacg
     5401 ccccgtcaag gggcgtcttg tggtgtctag cattgaatca ttggtaaatc acggtgcgta
     5461 gaagcgcgcc ttccagtagc cgggctcttt ttcgaccatg gtggcggtga catccacgat
     5521 ctccacccag gtcttgcccg gtttgagcgg gaagggattg ccttctccat ccacgaagcg
     5581 gatcggccgc agcaggccgc tcttcttttc gtagtcgccg gatacggtag accagtagat
     5641 cggatagacc ttgccatcgc gaaacaggta ggctctgttt gtggtgtaga acaggtccag
     5701 gtcgatcaag gtcctggctc cgttgaggac attgtgtttg gcatacagca caatgacgtt
     5761 ctcaaaggca agctgctcgc cggtgagccg gtctgtgctg ggatagaact ttccggatcc
     5821 gtcggctttg tccgggttgc gcaggtaggc accggaagcg gggtcgtact tccacaagat
     5881 ctggttcagg taggcgtaga atacccatag attggaggcg ttc
//
LOCUS       DBNHOH010000802         2031 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1653067, whole genome shotgun sequence.
ACCESSION   DBNHOH010000802 DBNHOH010000000
VERSION     DBNHOH010000802.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1653067"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="AC16V7_14420"
     CDS             complement(<1..186)
                     /locus_tag="AC16V7_14420"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6258365.1"
                     /translation="MFTLRPATSDDEAIIRDLVNAARINPTGLDWRRFVVATLTGGEI
                     IGCGQVKPHKDGSRELAS"
     gene            complement(229..1116)
                     /locus_tag="AC16V7_14425"
     CDS             complement(229..1116)
                     /locus_tag="AC16V7_14425"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6258366.1"
                     /translation="MFPVAGSSYLFLNELRLHYLHWNLADGKRFMVLLHGLASNARIW
                     ELVAPYLVERGFAVVAPDARGHGVTDKPDGDYDFDVFTRDLAALVNAFDIEHPILVGH
                     SWGASIVLDYAARFPFGRLAPAGIVLVDGGATQMDDLGLSWDEMRERLTPPRLAGLPV
                     EAFLERLESHNPRWKPDHHAMQIILANFEITEDETILPRLSFDNHMQIVRAMWEFKTY
                     ERFSRLNCPVLLVPARPTEPLSPQESQHLAFKERGVEEAMKRIQDVRVQWMEDAIHDI
                     PLQRPAELAARLADFAASL"
     gene            complement(1137..>2031)
                     /gene="gatB"
                     /locus_tag="AC16V7_14430"
     CDS             complement(1137..>2031)
                     /gene="gatB"
                     /locus_tag="AC16V7_14430"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF014674.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR6258367.1"
                     /translation="GVSEADMEKAHLRCDANVSICPKGTDILNPKTEIKNVNSIVAVR
                     QAIQTEVERQIREVGAGKRVEAWTLEWDEDAGVLRKMRSKETAADYRYFHEPDLLPLV
                     IDEDWKDEILANLPELPLERRNRFKQQYGLPEYDADILTGERSLSEYFEAAVTSYGGD
                     PKRVSNWLMNDVLRMINESGVPAGQLRLTPEYLAGIIKMVDAGTLNANTGKSLLDKVE
                     KTGRSPDEIVEAEGLAQLSDTAAIRGVCAEVIAENPEQVANYKAGKVTLMGWFVGQVM
                     KKSRGKADPQVARAMLEELLA"
BASE COUNT          462 a          597 c          540 g          432 t
ORIGIN      
        1 tgaggccagc tcgcgagagc catctttgtg aggtttgacc tgaccgcagc cgatgatctc
       61 cccaccagtc aaagttgcta ccacaaaacg ccgccagtcc agcccggtcg ggttgatccg
      121 ggcagcgttg accaggtctc ggatgatcgc ctcatcgtca gaggtagcag ggcggagggt
      181 gaacatagga taaaacaggc gctaacattc aaacttgcat ggaccaacct acagggaggc
      241 agcaaaatct gccagtcgcg cagccagctc agccgggcgc tgtagaggga tgtcgtggat
      301 agcatcttcc atccactgca cgcgcacatc ctggatacgt ttcatcgcct cttctactcc
      361 gcgctctttg aaagccaagt gttgactctc ttggggagag agcggctctg ttgggcgtgc
      421 aggcaccaac agcacagggc aattcaagcg agagaagcgt tcgtaggttt tgaattccca
      481 catggcgcgc acaatctgca tgtggttatc gaagcttagg cgtgggagaa tagtttcatc
      541 ttcagtgatt tcaaaattcg ccaggataat ttgcatggcg tgatgatcgg gcttccagcg
      601 agggttatga ctttccagac gttccaagaa agcctccaca gggagacccg ccagcctggg
      661 cggggtcaga cgctcgcgca tctcatccca gctcaagccc aaatcatcca tctgcgtcgc
      721 gccgccatcc accaggacga taccagccgg ggcaagccgg ccgaagggga atcgagcggc
      781 ataatcgagg acgatcgaag caccccaaga atgccccacc aagatagggt gctctatatc
      841 gaaagcattg acgagagcag ccaggtcgcg ggtgaagaca tcgaaatcgt aatcgccatc
      901 cggcttgtct gtcacgccat gaccgcgcgc gtcgggagcg acgacggcaa aacctcgctc
      961 caccaggtaa ggtgctacta gttcccaaat gcgagcgttt gaggccagtc catgcagcaa
     1021 caccataaac cgcttgccat ccgccaaatt ccagtggaga taatgcaagc gcagttcatt
     1081 aaggaataag taagacgatc cagccacagg gaacatgaat gagtcgacgg gccagcctaa
     1141 gccaacaatt cttccagcat cgccctggct acctgcggat cggctttgcc gcgggatttc
     1201 ttcatcacct gcccaacgaa ccagcccatc aaggtaactt tacccgcctt ataatttgcc
     1261 acttgctcgg gattctcggc gatcacttca gcgcacaccc cgcgaatggc agcagtatcg
     1321 ctcagctggg ccaacccctc tgcttcgaca atttcgtcgg gagagcgacc cgttttttct
     1381 actttatcca gcaaagactt gcccgtgttg gcgttgagcg tgccagcatc gaccattttg
     1441 ataatccccg ccagatattc aggtgtcagg cgaagttggc cagcaggcac accgctctcg
     1501 ttgatcatgc gcagcacgtc gttcatcagc cagttcgaga cgcgcttggg gtcaccaccg
     1561 tatgacgtta ccgctgcctc aaaatactca gacaggctgc gctccccagt cagaatatct
     1621 gcatcgtatt caggtaagcc gtactgctgt ttgaagcggt tcctccgttc caggggtaat
     1681 tcaggcagat tggccaggat ttcatctttc caatcttcat cgatgaccaa tggcagcaag
     1741 tccggctcgt ggaaatagcg gtaatcggca gccgtctcct tagagcgcat cttgcgtaga
     1801 acaccagcgt cctcatccca ctctagtgtc caggcttcga cgcgcttgcc ggcccccacc
     1861 tcgcgaatct ggcgctcgac ctcggtctgg atagcctggc gcaccgccac gatagagttg
     1921 acgttcttga tctcggtctt tgggttgagt atatcagtgc ctttcgggca aatagagacg
     1981 ttggcgtcgc agcgcagatg agccttttcc atgtcggctt cggagacgcc c
//
LOCUS       DBNHOH010000803         3915 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_562746, whole genome shotgun sequence.
ACCESSION   DBNHOH010000803 DBNHOH010000000
VERSION     DBNHOH010000803.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_562746"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="AC16V7_14435"
     CDS             complement(<1..1139)
                     /locus_tag="AC16V7_14435"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIR6258368.1"
                     /translation="MSYEYSNLEKIRLEKLERLRAQGVEPYPTRAERTHTSQQAIRLF
                     EQAESASGDEPVAVALAGRLRSMRPMGKITFAHIDDGDGRVQLFLRANDVGEEWLDLF
                     NHEFDLGDFVQASGEMFRTRSGEVSLRVQSIRLLAKAITPLPAAKDEVVDGQVVRHAT
                     LADPEARYRQRYADLAVNPDVRQIFRTRAAIMRAIREFLDGREFLEVETPVLQPLYGG
                     AAARPFITHHNQLKQDLYLRISFELYLKRLLVGNLERVYEIGRDFRNEGVDRTHNPEF
                     TMLEFYWAYADYLQVMELTEQMVVFVADHVLGHRKLTFQGKEINLEPPWPRIPLRKAL
                     LDKSDIDIDLYPNAESLMETMRGRGLQPDPKATRGKLIESLMGDYL"
     gene            complement(1219..1680)
                     /gene="greA"
                     /locus_tag="AC16V7_14440"
     CDS             complement(1219..1680)
                     /gene="greA"
                     /locus_tag="AC16V7_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016476590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIR6258369.1"
                     /translation="MPPSFLTKEGHKKLQEELDYLRTVKRQEVAQRLHEAMEGGELIE
                     DAEYEAAKNEQAFVEGRIQELEILLANARVIDESKNGDVVQVGSKVTIQEDKNDPETY
                     IIVGPAEANPRNGRISNESPLGRALMEHRAGDNVRVEAPGGAFVVKVVKVG"
     gene            complement(1894..2805)
                     /locus_tag="AC16V7_14445"
     CDS             complement(1894..2805)
                     /locus_tag="AC16V7_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6258370.1"
                     /translation="MEVFLVGLIAATLRVATPLIFGTLGELFSERAGILNLGIEGTMF
                     LGAFVGFAVAHTTGSLWLGFFAAVLAGILAGLLMGLLTVSLGLNQHVSGLGLTLFLTG
                     LSLFAYRLIFGERSLPPSISPFPKLSLFGNFPVLGPVFEQYALTYLAIALVLLFWWLI
                     YRTSFGLQLRAVGENPEAADAAGVNVFMMRYLALGVAGGLMAGGGAFLSLAQLGSFIF
                     GIIAGRGWVCIALIIFANWQPSKVFWGALLFGGVFALQLRLQATGIRLPYELFLALPY
                     LVTIAALTIAGRNATAPSALLKPYRRE"
     gene            complement(2805..3857)
                     /locus_tag="AC16V7_14450"
     CDS             complement(2805..3857)
                     /locus_tag="AC16V7_14450"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6258371.1"
                     /translation="MPRLRFEKRAAPLWTRSLLPLMAVGVTFVLTSLLIFWAKANPFE
                     AYYYFLISPLSSRVSALEVLVKATPLLLTGAAVTFAFAAGYWNIGAEGQLLAGAIAAS
                     GLGIALENVSPFLAIPIMLVGGFLAGMLWASIPALLKVKLAVDEVVTTLLMNTVILFL
                     ISFLLNGIWRDPVSGWPQSPQIAEAATFPRLILRSRLHLGFLVAWIVVGFMWVVLYRT
                     PLGLRMRAVGLGQSAANFAGVNVGRTILIAALVSGGIAGLAGVSEVAGIHFHLIEAIS
                     PGYGYTGIIVATLGGLNPLGVSLAAIFIGLINTGAQSVSRALGVPIFLGNVVEAVLLL
                     VTLSMLILQNYRIKVA"
BASE COUNT          967 a         1190 c          989 g          769 t
ORIGIN      
        1 aggtaatcgc ccatcagcga ttcgatcaac ttgcctcgcg tggccttcgg atcaggctgc
       61 aggccgcgcc cacgcatggt ttccatcagg ctttcagcgt tggggtacag gtcgatatcg
      121 atatcgctct tatccagcaa tgccttgcgc aacgggatac gtggccaggg aggctccagg
      181 ttgatttcct taccctggaa cgtcagtttt ctatgtccaa gcacatgatc agcgacaaaa
      241 actaccatct gctcagtcag ctccatcacc tgcaggtagt cggcataagc ccagtagaac
      301 tccagcatgg tgaattcggg gttgtgagta cggtccacgc cctcattgcg gaaatctcgc
      361 ccaatctcat agacgcgctc caggttgccc accagcaagc gctttaggta aagctcgaag
      421 gaaatccgca ggtatagatc ttgcttgagc tggttgtggt gggtgataaa cggccgggct
      481 gccgcgccgc cgtacagcgg ctgcagaacc ggggtctcca cctcgagaaa ttcgcgccca
      541 tccaggaact cgcgtatagc ccgcatgatg gctgcccgcg tgcggaagat ttggcggaca
      601 tcgggattta ccgccagatc agcatatcgc tgacggtagc gagcttcggg atctgccaac
      661 gtggcatggc gcaccacctg cccatccacc acctcgtctt tggccgccgg taagggggta
      721 atggccttgg caagcagccg aattgactgc acgcgtaggc tcacttcgcc agagcgggtg
      781 cggaacatct ctccagaagc ctggacgaag tcacccaggt caaattcatg gttgaacaga
      841 tcgagccact cctcccccac atcattggct cgcagaaaga gctgtacgcg gccatcgccg
      901 tcgtcgatat gtgcaaaagt gatcttgccc atcggacgca tggagcgcag tcggcccgcc
      961 agggcgactg caactggctc atctcccgat gcagattctg cctgctcgaa gaggcggatg
     1021 gcctgctggc tggtgtgcgt gcgctccgct cgggttgggt agggttccac gccctgcgct
     1081 cgcagtcgct ctagcttctc caggcggatc ttttccagat tggagtattc atacgacatt
     1141 gactgcattc cttccaatat taagcaaaag ccccgcgcaa gatgattacc tgcgcggggc
     1201 tatatggtca gccccaaatc agccaacctt aacaactttt acaacaaacg ccccacctgg
     1261 agcctcaaca cgcacattat cgcctgcgcg gtgctccatc agggcacggc ctagaggcga
     1321 ctcattcgat atgcgtccat tgcgagggtt agcctccgcc ggcccgacaa ttatgtaggt
     1381 ttctgggtcg ttcttgtctt cctgaatggt gaccttggaa cctacctgga cgacatctcc
     1441 attcttagac tcgtcgatca cgcgcgcgtt ggccagaaga atttccagct cctggatacg
     1501 cccttcgaca aatgcctgct cgttcttggc ggcttcgtat tcagcatctt cgatcagctc
     1561 gccgccttcc atggcctcgt ggaggcgttg ggctacctcc tggcgcttga cggtacgcag
     1621 gtaatcgagc tcttcttgta atttcttatg gccctcttta gttagaaatg aggggggcat
     1681 gtcaacatcc tttacttaga gataataaaa tcactcctcg agagcgattt ttccagtacc
     1741 actacttttt agaacaaaag aaccgcggcc atcgctggaa taaatccctg ctgcgccgcg
     1801 ccaagatttt agcacaattc actatgaaat gcaagattta gaaaaaggcg atctggactg
     1861 atttccttac aatttattaa ttttgttagg atcttattcg cgccggtaag gtttcaagag
     1921 cgccgacgga gccgtggcgt tccggcctgc tatggtcaat gccgcaatcg tcaccaagta
     1981 gggcagcgcc agaaacagct cgtacggcag cctaattccg gtggcttgca ggcgaagttg
     2041 cagggcaaaa accccaccaa aaagcagggc gccccaaaac accttggatg gctgccagtt
     2101 cgcaaaaatg atcagcgcga tgcacaccca gccgcgccca gcgataatgc cgaagatgaa
     2161 ggaacctagc tgcgccagtg agaggaatgc cccaccgcct gccatcaacc cgccagcaac
     2221 acccagcgcc aggtaacgca tcataaatac attgaccccc gccgcgtcgg ccgcctcagg
     2281 gttttcgccc actgcccgta attgaaggcc aaagctggtc ctgtaaatca gccaccagaa
     2341 tagcagcacc agcgcgatcg ccaagtaggt gagcgcatat tgctcgaaga ccggtcccag
     2401 gactggaaag ttcccgaata aggagagttt cggaaatggg gatatcgatg ggggcaaact
     2461 tcgctcgcca aaaatcaggc ggtaggcgaa gagtgataga cccgtcaaaa acagggtaag
     2521 ccccaacccg gagacgtgct ggttcaagcc caggctaacc gtgagcaaac ccatgagtaa
     2581 acccgccaag atgccagcca gtactgccgc aaagaaaccc aaccataagg aaccggttgt
     2641 atgagccacc gcaaagccga caaacgcccc caggaacatg gtgccttcaa tgcccaggtt
     2701 caaaattccg gcgcgttcag agaataattc acccaaagtt ccgaaaatca atggcgtcgc
     2761 cacgcgcagt gtagccgcta tcaatccgac cagaaaaact tccatcacgc cacctttatc
     2821 ctataattct gcaggataag catgctcaag gttaccagta gcagaaccgc ttcgaccaca
     2881 ttccctagga aaatgggcac tcccagggcg cggctgacag actgagctcc cgtgttgatc
     2941 aaaccgataa atattgccgc caggcttacc ccgagcggat tgagcccacc cagcgtagcg
     3001 acgataatgc ctgtatatcc atagccaggg gagatggctt cgatcagatg gaaatgaatg
     3061 cccgccacct cgctcactcc cgccagacct gcaataccac cgctgaccag ggcagcgatc
     3121 aaaatggtcc gcccaacgtt taccccagcg aaattagccg ctgactgccc gagtcccact
     3181 gctcgcatcc gcaggccgag cggcgtgcga tataaaacga cccacatgaa cccaaccacg
     3241 atccaggcca ccagaaaccc caggtgcagc cttgaacgca aaatcaatct tgggaacgtg
     3301 gcagcctccg caatttgagg cgactgcggc cagccagaga ctggatcacg ccagattcca
     3361 ttgagaagaa agctaatcag gaataagatg accgtattca tcagcagggt ggtcaccacc
     3421 tcatccactg ccagtttcac tttgagcagc gcaggtatcg acgcccacag catccctgcc
     3481 aaaaaccccc caaccagcat gatagggatt gccaggaagg gagaaacatt ctctagcgct
     3541 atccccaagc cagacgccgc aattgcccca gccaaaagct ggccctcagc gccgatattc
     3601 caatacccag ccgcaaacgc aaacgtgacc gccgcccccg tcagcagcaa gggggtagcc
     3661 ttaaccagca cctctagcgc actcacccta ctagagagag gggagatgag aaaatagtaa
     3721 tacgcctcga atgggttagc cttggcccag aagatcagca gcgatgtcag gacaaaagtg
     3781 acccccaccg ccatcaacgg cagcagcgag cgcgtccaaa gcggggctgc cctcttttca
     3841 aacctcaacc taggcatgat ccacctccag cgcgcccgac atcatcaatc ccaactgctc
     3901 gatgtccgct tcttg
//
LOCUS       DBNHOH010000804         2579 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1782125, whole genome shotgun sequence.
ACCESSION   DBNHOH010000804 DBNHOH010000000
VERSION     DBNHOH010000804.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1782125"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            330..641
                     /locus_tag="AC16V7_14455"
     CDS             330..641
                     /locus_tag="AC16V7_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258372.1"
                     /translation="MGLFKKIAQMFTPSEREDDRAYWLYVQCGRCGEKIRARVDLYND
                     LSPLYHEAGVSYFSRKVLMGQQHCFQKVEVEMNFDQNRRLTARKISGGSFITEEDFGS
                     G"
     gene            complement(673..1263)
                     /locus_tag="AC16V7_14460"
     CDS             complement(673..1263)
                     /locus_tag="AC16V7_14460"
                     /inference="COORDINATES: protein motif:HMM:NF025636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6258373.1"
                     /translation="MNATPLSRHERRRLQTRKLLMQATLQLVLEKGFDAISIQDITDR
                     ADLGRGTFYIHFKDKEEVVWTAFRELFQELEQEAHNQLDRSMPQVEYYGLLNIFRHAE
                     NNRDLYRVMFGGQGSAILTARAQDFLAQAFLYDIRNAPQPPEINFNIPEEIEAQILTG
                     VISRLLFWWLGTSNSYSTEQMAVMTYEALYRKKPPN"
     gene            1440..2270
                     /locus_tag="AC16V7_14465"
     CDS             1440..2270
                     /locus_tag="AC16V7_14465"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015998.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6258374.1"
                     /translation="MSETTNLNLSGVAETLLITLYIRAMESQRPDALIKDEKAVALVT
                     QMGYDFSRIEQIKMDEEDKVALILRNLEFDRYTRDFLARNPDAVTVHIGCGLDSRFER
                     VDNGTVEWYDLDLPDVIELRRKFIGGEGGRYHLLGCSVFDEAWLKAVSAYQQRPFLFL
                     AEGVFMYFEEAQVKSLVMTLLEHFPGSELVFDAYSPYLVWANNLRLSLTKFGARYHWG
                     LKRGKDLESWGPGICLLDDWSYFDRPEPRLAHIRWMRHIPFLARVLCIYHYQLGGATR
                     "
BASE COUNT          620 a          631 c          675 g          653 t
ORIGIN      
        1 aacaaccttg cccgtggcct gtgtttagct gatttgatgg catagctgtg aagattattc
       61 gccggtattg ccgtcaatgc tgtttaccaa tccatctttt accaattttg gaacggtcac
      121 gcccaaatcc cctttcctac tcgagctgcg taaccagggc aattgcatat gccctgaggg
      181 gcatgcgcaa tcccgtaaaa ccagggtttt gggtgttttg gtgggggtat gccgccacca
      241 aaacacccat atttctctcc tcagtggtga gaaatcactc taatgcgatt gccctatcac
      301 attcacggct tagaaaaagg agacccgtca tgggcctttt caaaaagatc gcacaaatgt
      361 ttaccccgtc agaaagggaa gatgaccgcg cctattggtt gtatgtccag tgcgggcgct
      421 gcggcgagaa gatacgggca cgcgtggatc tgtacaatga cttaagcccg ttgtaccacg
      481 aggcaggtgt cagctatttc agccggaagg tcttgatggg ccagcaacat tgcttccaga
      541 aggttgaagt cgagatgaat ttcgaccaaa accgccgctt gacggcgcgc aagatttccg
      601 gggggagctt tatcacggaa gaagacttcg gtagcgggta aaacaaaatc agcagagatt
      661 gggtgcaaat acctaattgg gtggcttttt gcgatatagc gcctcgtatg tcatgaccgc
      721 catttgctct gttgaatagg aatttgatgt cccaagccac cagaagagca aacgggaaat
      781 cacaccagtt agaatctggg cctcaatctc ttccggtatg ttgaaattga tctcaggggg
      841 ttgtggcgcg ttgcgtatat catataagaa ggcttgtgcc aggaagtctt gcgcgcgcgc
      901 agtcagtatt gcggatccct gcccgccaaa catcacccgg tataagtcac ggttattctc
      961 tgcatggcgg aagatattca gaaggccgta gtattctacc tgaggcatgc tgcgatcgag
     1021 ttgattgtgt gcttcctgct ccaactcctg aaataattcc cgaaacgctg tccagactac
     1081 ctcttcttta tctttgaagt ggatataaaa tgtgcctcga cccaggtctg cccggtcggt
     1141 aatatcctga atcgaaatgg cgtcgaatcc tttttcgaga accagttgca aagtagcctg
     1201 catcaacagc ttgcgtgtct gcaagcggcg gcgttcatgg cgagaaagcg gggtagcgtt
     1261 catggttgta cacctgttca ttcatgacgg cgagcaaaaa ctcatgcggt caatttcttg
     1321 acgagtaggt tgaaacgtat atactaatta tgacaattat atcatatatg acgttgtgtt
     1381 caataacgcg ctacgtattc gtgtatagat cgcaaaattt tcacctaggg aagatgaaca
     1441 tgtctgagac tactaaccta aacttgagcg gagtggctga aactttgctg attacgcttt
     1501 atatccgagc catggaatcg caacgtcctg acgcgctgat caaagacgag aaagccgtgg
     1561 ccctggtcac acagatgggc tacgatttct cgcggatcga gcagatcaag atggatgaag
     1621 aggataaagt agccctgatc ctgcgcaacc tggaattcga ccgctacacg cgggattttc
     1681 tagcccgcaa tccagatgca gttacagtgc atattgggtg tggcctcgat tcgcgctttg
     1741 agcgcgtgga caatggcaca gtagaatggt atgaccttga tttaccggac gtcattgaac
     1801 tgcgccgcaa gttcatcggt ggcgaagggg ggcgctatca cttactgggg tgttcggtgt
     1861 tcgatgaagc gtggctgaag gcggtgagcg cgtatcaaca gcgcccattt ctctttctgg
     1921 ctgagggcgt ttttatgtac tttgaagaag cacaggtcaa gtcgctggta atgactttgc
     1981 tggaacattt tccaggctcg gagctggtct tcgatgccta ttcgccatac ctcgtctggg
     2041 cgaacaacct gcgcttatcc ctcaccaaat tcggtgctcg ctatcactgg ggtctgaaac
     2101 gtggcaaaga tctcgaaagc tggggtccgg gcatttgcct gctcgacgat tggagttact
     2161 tcgatcgccc tgagccgcgc ctggcccata ttcgctggat gcgccatatc ccattcttgg
     2221 ccagggtctt gtgcatttac cactaccagc ttggaggggc aacaagataa ggaaaatctg
     2281 cgatcaacgg ttgaaccact atgcaagcac ctcaatgaca gccttctccg cgtctacccg
     2341 caacttttcg cctgtcctta ttttttcaat ggaaatgtga tccacgcagg gtatctcggc
     2401 gatgatgcag cctacttggg tcatcgccgc gcagatgcag atgctcgacc gcaagcccga
     2461 ggaagagaag gccgtcctgg aagaaaacga tctggcttga aatcctgtca tcgcgagtaa
     2521 ctccacagcg cacggcgctg ttctttcgag aggaaattgt cattgcttca cctgccgta
//
LOCUS       DBNHOH010000805         1297 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_756596, whole genome shotgun sequence.
ACCESSION   DBNHOH010000805 DBNHOH010000000
VERSION     DBNHOH010000805.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_756596"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="AC16V7_14470"
     CDS             <1..410
                     /locus_tag="AC16V7_14470"
                     /inference="COORDINATES: protein motif:HMM:NF012415.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HIR6258375.1"
                     /translation="RDTTVEAQVMSLVNQIRQNNGLASFNTSSPLSEAAYVHSRDMAC
                     NRFVDHSGSDGSSWYDRIRAQGYAFSNANENIYVGFPEFGGTAEGAVNWWMNSQVHRD
                     NILKADNVDAGVGYVYDPNSQWGGYYTMVFARP"
     gene            1040..>1297
                     /locus_tag="AC16V7_14475"
     CDS             1040..>1297
                     /locus_tag="AC16V7_14475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6258376.1"
                     /translation="MKLLLVDDHILFREGLKSLLGSQPGFQIVGEAATGEEAIAKTRS
                     QHPDLVLIEIGLKDGSGLETIGNILSHRHETKVVVLTLLELD"
BASE COUNT          358 a          301 c          310 g          328 t
ORIGIN      
        1 agcgagatac gacggttgaa gcacaggtca tgtctctagt caaccagatt cgccagaata
       61 acggcctggc ttccttcaac acatcctcgc cattatcaga agctgcgtat gtgcacagcc
      121 gagacatggc ttgcaaccgc tttgttgatc acagtggctc agatggctcg tcctggtatg
      181 atcgcatccg cgctcagggg tacgctttca gcaacgccaa cgagaatatt tatgtcggct
      241 ttccagagtt cggcggcacc gccgaagggg cggtaaactg gtggatgaac agccaggtgc
      301 accgcgacaa tattctcaaa gccgataacg tcgatgccgg tgtaggctat gtgtacgatc
      361 caaatagcca atgggggggc tactacacga tggttttcgc ccgtccatag ataaatgtta
      421 ttcgcagctc caatagaata tccggcgacc agctggatta tcgaaaagat ttgatctatc
      481 ttgaggggac acagcaatac aaatgaagag cgctacaggc tgtgtgttga ctcgttaatc
      541 agccctggac ttccaaaatt tccataaaca tctgacaaat ttctgaaatc aatctttatc
      601 ccttacattt gactggggcg actgtaacat tagggatgtt tacccgttgt attaatcaac
      661 aattaagtac accgcgatca tgcgagggat cctgaattcg atcgacaact tgcctgtgat
      721 gctctaatca gagcaactat tccggcaaaa tttggatcaa caagttgaaa cgatgaaaag
      781 ggaggtgtgt agaattttcg aagtagcctt tacttaatca ccaaaaccag caaagtgagc
      841 agagaatcga tgaccagatt tggtcgctta tcctacctca acaggtaggt gaggtcagcg
      901 aggagccagt agcgaaacca cccctaaggt ggattttact ttaacaacag aattcttctt
      961 gttttcatct gatcttcaat tctaattgga tgatcctcgc ccagatctaa tcttttattc
     1021 atcgaatatc gtttatcaca tgaaattatt acttgtcgac gaccatatac tatttaggga
     1081 ggggctaaag agcttgcttg gcagccagcc cggcttccaa attgtgggag aggctgccac
     1141 tggtgaagaa gccattgcta agacgcgctc acagcaccct gatttagtat tgatcgagat
     1201 cggcctgaag gatggaagcg ggctggaaac gatcggcaat atcctttcac atcgacacga
     1261 gacgaaggtg gtggtactca ccctgcttga actggac
//
LOCUS       DBNHOH010000806         3883 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1718022, whole genome shotgun sequence.
ACCESSION   DBNHOH010000806 DBNHOH010000000
VERSION     DBNHOH010000806.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1718022"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..148)
                     /locus_tag="AC16V7_14480"
     CDS             complement(<1..148)
                     /locus_tag="AC16V7_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003178358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIR6258377.1"
                     /translation="MGTIKSRSVARLRRHRRVRKNVSGTPEKPRLNVFRSLAEIYAQV
                     IDDEA"
     gene            complement(152..694)
                     /gene="rplF"
                     /locus_tag="AC16V7_14485"
     CDS             complement(152..694)
                     /gene="rplF"
                     /locus_tag="AC16V7_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010896332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIR6258378.1"
                     /translation="MSRIGRAPVDVPNGVDVKIKGSNVRVKGPKGELARTFPADIIIS
                     VEDGKIVVKRPSDEPRHRALHGMTRALINNMVVGVSDGFEKILEVNGVGYRAELDGEN
                     LVLYVGYSHSVKMEPPGGISFEVDARTRQIKVMGVDKEMVGQVAANIRMVRPPEPYKG
                     KGIKYLDERIRRKAGKAGKV"
     gene            complement(706..1119)
                     /gene="rpsH"
                     /locus_tag="AC16V7_14490"
     CDS             complement(706..1119)
                     /gene="rpsH"
                     /locus_tag="AC16V7_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIR6258379.1"
                     /translation="MSVNDPIADMLTRIRNAVMVGHQMVALPSSKLKVAIAKILKDEG
                     FITSFEVVDGKTVGQKVLRIRLKYIGERRERKPVITGLERISRPGRRVYTNKREIPWV
                     LSGMGVAIISTPKGVMTGQRARQLGVGGEILCKVW"
     gene            complement(1139..1321)
                     /locus_tag="AC16V7_14495"
     CDS             complement(1139..1321)
                     /locus_tag="AC16V7_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002557082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HIR6258380.1"
                     /translation="MAKKCMMIRETRRKYPTRVRNRCKQCGRPRGYLRRFGLCRICFR
                     ELALEGKIPGVVKSSW"
     gene            complement(1325..1876)
                     /gene="rplE"
                     /locus_tag="AC16V7_14500"
     CDS             complement(1325..1876)
                     /gene="rplE"
                     /locus_tag="AC16V7_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIR6258381.1"
                     /translation="MMGHILKERYQEEIVPALMKSLHLENNMQAPRIQKIVVNVGVGE
                     ALDNAKALDATVADVTQVTGQKPIVTKARKSIANFKLREGRQIGVKVTLRGDRMWAFL
                     DRLMNVALPRVRDFRGISPNAFDGRGNYTLGLREQLVFPEIDYDKIDKLRGMEISIVT
                     TGRNDEEGRQLLQLLGMPFKKEG"
     gene            complement(1898..2233)
                     /gene="rplX"
                     /locus_tag="AC16V7_14505"
     CDS             complement(1898..2233)
                     /gene="rplX"
                     /locus_tag="AC16V7_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIR6258382.1"
                     /translation="MRKKIRKGDMVEVINGRFEDKGKRGEVIRVNPDDGRVVVQGINI
                     RKKHQRQVQTQGRRNLSPGIIEFEGPIHISNVMLVCPKCSEPTRVGLQREGDESLRVC
                     KHCHGLIDV"
     gene            complement(2260..2631)
                     /gene="rplN"
                     /locus_tag="AC16V7_14510"
     CDS             complement(2260..2631)
                     /gene="rplN"
                     /locus_tag="AC16V7_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIR6258383.1"
                     /translation="MIQQESRLKVADNTGARELLVIHVMGGSTRRYARVGDVVVATVK
                     AATPQGSVKKSDIVRAVVVRCAKEWRREDGSYIRFDDNAAVILDQDGRNPKGTRIFGP
                     VARELREKGFMKIVSLAPEVL"
     gene            complement(2628..2915)
                     /gene="rpsQ"
                     /locus_tag="AC16V7_14515"
     CDS             complement(2628..2915)
                     /gene="rpsQ"
                     /locus_tag="AC16V7_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIR6258384.1"
                     /translation="MNTRRRLTGVVTSDKMDKTVVVEISRTYRHRLYKKVVDSSKRIM
                     AHDELGCQVGDHVRIVESRPISRHKHWVVEEILRRAIGADEPEVGLLENVP"
     gene            complement(2912..3124)
                     /gene="rpmC"
                     /locus_tag="AC16V7_14520"
     CDS             complement(2912..3124)
                     /gene="rpmC"
                     /locus_tag="AC16V7_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002509269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIR6258385.1"
                     /translation="MKPEEIRALSTEDIQARLDDAKEELMKLRFQLAMGGLNDYTRLR
                     YTRKSVARLMTILNERQFTAQEEGEA"
     gene            complement(3121..3555)
                     /gene="rplP"
                     /locus_tag="AC16V7_14525"
     CDS             complement(3121..3555)
                     /gene="rplP"
                     /locus_tag="AC16V7_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIR6258386.1"
                     /translation="MLMPKRVKWRKQMRGRMRGKAQRGAQISFGEFGLQAMEPGWVSA
                     RQIEAARRAIVRALRRRGKVWIRIFPDKPVTRKAAETRMGKGKGNVEYWVAVVKPGRV
                     MFEVGGGLTPDVAQEALRLAQYKLAIKTKIIGRYEETGGGEA"
     gene            complement(3565..>3883)
                     /gene="rpsC"
                     /locus_tag="AC16V7_14530"
     CDS             complement(3565..>3883)
                     /gene="rpsC"
                     /locus_tag="AC16V7_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIR6258387.1"
                     /translation="ADQLERRISYQRAMKRAIQQAIRQGAEGIRLEVSGRLSGAEMAR
                     AVNMREGQVPRQTLRADIDFSKAEANTTYGRIGVKVWIYKGEVLPEMEDKLESTEGVY
                     VSE"
BASE COUNT          858 a         1182 c          862 g          981 t
ORIGIN      
        1 cagcctcgtc atcgattacc tgggcgtaga tctcggccag gctgcgaaat acattcaagc
       61 ggggtttttc tggcgtcccg ctaacatttt tacggacacg tcgatgccgt cgcaaccgtg
      121 ctactgaacg tgatttaatc gtacccatag attacacctt gcctgcctta ccagctttac
      181 ggcgaatcct ctcgtcaagg tatttgatgc ctttaccctt atatggttca ggcgggcgca
      241 ccattcggat attggcggca acctgtccaa ccatttcttt atctacaccc atcaccttga
      301 tttgtcgagt gcgggcgtcc acttcaaaag aaataccacc gggcggctcc atcttcaccg
      361 aatgagagta gccaacgtaa agaaccaaat tttctccatc caactcagcg cggtaaccca
      421 cgccgtttac ttccaggatt ttctcaaatc catcactgac gcccacaacc atattgttga
      481 tcagcgcccg tgtcatgcca tgcagggcgc gatggcgggg ttcgtcgcta gggcgcttca
      541 cgacgatctt tccgtcctcg accgaaatga tgatatcggc cgggaaggtg cgcgccaatt
      601 cgcccttggg acctttcacc cgcacgttgg agcccttaat tttcacatct accccattgg
      661 gtacatcgac cggtgcacga ccaattctcg acacagatac ctcctttacc agaccttaca
      721 aaggatctcg ccgccaaccc ctaattggcg agcacgctgg ccggtcatca cacccttggg
      781 tgttgaaata atggcaacgc ccattccaga taatacccaa gggatttccc gtttgttggt
      841 ataaacccta cggcctggac ggctaatccg ttccagtcca gtaattacag gttttcgttc
      901 gcgccgctct ccaatatact ttaagcggat gcgtaagact ttttgcccta cggttttgcc
      961 atctaccact tcgaaggaag tgataaatcc ctcatctttg aggattttgg cgatcgccac
     1021 tttgagcttg gagctgggta aagccaccat ctggtgacct accatgaccg cattgcgaat
     1081 gcgggtcaac atgtctgcga taggatcatt cacactcatt ctcaaaccct ccaaccggct
     1141 accaggatga cttcactacg ccagggattt tgccttctaa tgccaactca cggaagcaaa
     1201 tgcggcataa accaaaccgg cgcagatacc cgcgcggtcg cccacactgt ttgcagcgat
     1261 ttcggacacg ggtagggtac ttccgcctgg tttcacggat catcatacac tttttggcca
     1321 tcgcctaacc ttccttcttg aacggcatgc ccagcaattg cagcaattgc cggccttctt
     1381 catcattgcg accagtcgtc acaatactaa tttccatgcc acgtaatttg tcgatcttgt
     1441 cgtaatcgat ctctggaaat accaactgct ctcgcaatcc caaggtgtag ttgccgcgcc
     1501 cgtcgaacgc attcggcgag atgccgcgga aatcacgcac gcgtggcaaa gcaacgttca
     1561 tcaagcgatc caagaatgcc cacatacggt cgccgcgcaa cgtgactttc acgccgattt
     1621 gccggccttc acgcaactta aagttcgcaa tgctcttgcg ggctttagtc acgatgggtt
     1681 tttggccggt cacctgggtg acatccgcca cggtggcgtc cagtgctttg gcattatcca
     1741 aggcttcgcc gactcctaca ttaacaacaa tcttttggat gcgcggcgcc tgcatattat
     1801 tttccagatg aagcgacttc atcaatgccg gcacgatctc ttcttgatac ctttctttca
     1861 atatgtgtcc catcatttcg ctcccagata ggccatctta tacatcgatc agcccatggc
     1921 aatgtttaca aacacgtaaa gattcatcgc cttcacgctg taaacccaca cgggttggct
     1981 cgctacactt cgggcaaacc aacatcacgt tggatatatg gatcggccct tcaaactcaa
     2041 tgatgcccgg agacaggttg cgccgaccct gggtctggac ctgccgttgg tgctttttac
     2101 gtatattaat gccttgcaca accacacgtc catcatcagg atttacgcgg atcacctcac
     2161 cgcgcttacc tttatcttca aagcgaccat tgatcacttc aaccatatcg cctttgcgaa
     2221 ttttctttct cacaatacgc tctcctctcc gcagagtcct tacagtacct caggggctag
     2281 cgagacgatt ttcatgaatc ccttctcgcg cagttcacgg gcaacaggtc cgaagatgcg
     2341 ggtaccttta ggatttctgc catcctgatc caggatgacc gcggcattat catcgaagcg
     2401 gatataggac ccgtcttctc gccgccactc cttcgcgcag cggacaacaa ctgctcgcac
     2461 aatatcactc ttcttcaccg agccctgcgg ggtagctgcc ttgaccgtag ctaccaccac
     2521 gtcgcccact cgggcataac ggcgggtaga cccacccatc acgtggatga ccagcagctc
     2581 gcgtgcgccg gtattatcgg caaccttcaa gcgagattcc tgctggatca tggcacattc
     2641 tccaaaagac ccacctcggg ctcgtcagca ccgattgcgc ggcgcagtat ctcttcaaca
     2701 acccaatgct tgtggcgaga aatcggtcgg ctctccacaa tacgtacatg atctccaacc
     2761 tggcaaccca attcatcgtg ggccataatc cgcttgctgg aatcgactac ttttttatac
     2821 agtcggtggc ggtaggtacg ggaaatttcc acaacgactg ttttatccat cttgtcactg
     2881 gtaaccacac cagttaagcg acggcgagtg ttcatgcttc accttcttcc tgggctgtaa
     2941 actggcgctc gttaagtatt gtcattaggc gcgcaaccga cttgcgagtg tagcgaagac
     3001 gggtgtaatc attcagaccg cccatggcca gttgaaagcg cagcttcatt aattcttctt
     3061 tcgcatcgtc caggcgagcc tggatatcct cagtggaaag agcgcgtatt tcctctggtt
     3121 tcatgcctcg cctcctcctg tctcttcata gcggccaata attttagttt tgatggccag
     3181 cttatactgg gctaggcgca atgcctcttg agccacatct ggggtcaaac caccacccac
     3241 ttcgaacatg acccggcctg gtttcacgac cgcaacccaa tattccacat tgcctttacc
     3301 tttacccatt cgcgtttccg ctgctttgcg agttaccggc ttatcaggga aaatacggat
     3361 ccatactttt ccacggcgtc gcagagcgcg cacaatcgca cgtcgggcag cttcaatctg
     3421 gcgtgcgctg acccagcccg gttccatagc ctgcaggcca aattctccaa aagagatttg
     3481 cgcaccacgt tgagctttgc ccctcattcg accgcgcatc tgcttgcgcc actttacacg
     3541 ctttggcatt aacatgatcg caccttattc actaacgtat acaccttcag tcgactcgag
     3601 cttatcttcc atctcgggca gtacttcgcc cttatagatc cagactttaa ccccgatccg
     3661 accgtaggtg gtattggctt cagctttgga aaagtcaatg tcggcgcgta atgtctggcg
     3721 cggcacctga ccctcgcgca tatttaccgc ccgagccatt tcagcaccgc ttaaccgacc
     3781 agagacctcc aaacgaatcc cctcagctcc ctggcggatc gcctgctgga tggcgcgttt
     3841 catggcgcgt tggtagctaa tccggcgctc aagctgatct gcg
//
LOCUS       DBNHOH010000807         2649 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_562982, whole genome shotgun sequence.
ACCESSION   DBNHOH010000807 DBNHOH010000000
VERSION     DBNHOH010000807.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_562982"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1298)
                     /locus_tag="AC16V7_14535"
     CDS             complement(<1..1298)
                     /locus_tag="AC16V7_14535"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR6258388.1"
                     /translation="MSLLTTSNLSKSYGPRDIFTGIDLSLPHGARIALVGPNGIGKTT
                     LLRILIGLEEASSGTLSRARNLQVGYLPQEAILHGTHTLWEECLRAFEELRSLENELT
                     RLEGLMSDQEQAEAALERYGPLQVDFELRGGYIYETRIRQTLSGLGFLPADFHRPIPQ
                     LSGGQRTRAVLARLLLSDPDLLILDEPTNHLDISAVEWLEGYLRQWSGAALIVSHDRY
                     FLDQVVDHIWEMSTTGLETYRGNYTAYVNVRHERWELRQQLFRDEKERLLKELDYVKR
                     NISGQRTLQAKGKLRRLSRQVEAIESLGVEAVLNKSWGEVSEKADISSHPMNVDEVER
                     HIRALRPPSHRPPRLHLNLRSGQRSGDLVVRTYEVDIGYADEGRPLFHVPDLVFKRGE
                     CAALIGPNGAGKTTFLKTLLGMLAPLAGEVVMGASLNVAY"
     gene            1728..>2649
                     /locus_tag="AC16V7_14540"
     CDS             1728..>2649
                     /locus_tag="AC16V7_14540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR6258389.1"
                     /translation="MEQMQRNDKLIEQSGSSKTVNRLKLALLAAFIIAAGITAYITFV
                     FVRDFVTSWELTSLPGITIRDEKPTSTPDASGVIQDVQTPLQQVGGPTPEPWDGASRV
                     TLLVMGLDYRDWEAGEGAPRTDTMMLLTINPLDRTAGILSIPRDLWVNIPGFNYGRIN
                     TAYSLGEAYKYPEGGGPGLAMATVEELIGVPVDYYAQIDFGAFIRFIDEINGITITIE
                     KRMKVDQIGGKVKLLRPGTYTLDGELALGYARARNTEGGDFDRAFRQQEVIIAIRKRL
                     LESDNLPNLIAKSPVLYQELSSGVHTNLNLD"
BASE COUNT          584 a          772 c          716 g          577 t
ORIGIN      
        1 aagtaagcta catttaagct ggcgcccatc accacttcgc cagccagggg ggcgagcatc
       61 cccagtaatg ttttgagaaa cgtggttttt ccagctccat ttggaccgat cagggcggcg
      121 cactcgccgc gcttaaagac cagatcgggg acatgaaaga gcgggcgtcc ctcatcggca
      181 taaccgatat ccacctcgta ggtacgcact accaagtcgc ccgagcgctg cccagagcgc
      241 aaattcaggt gcaagcgagg cgggcgatgg ctgggtgggc gcaaggcgcg gatatgccgc
      301 tccacctcgt ccacgttcat tggatgagac gagatatccg ccttttcgct gacctcgccc
      361 caacttttgt tcaagaccgc ctcaacaccc aggctctcga tggcttccac ctggcggctc
      421 agccggcgca gcttaccctt cgcctgcagc gtccgctgtc cggagatatt gcgcttgaca
      481 tagtccagct ccttgagcaa gcgctctttc tcgtcacgaa acaattgctg gcgcagctcc
      541 cagcgctcat ggcgcacgtt cacgtaggca gtgtagttgc cgcggtaggt ttccaggccg
      601 gtggtgctca tctcccaaat atggtccacc acctggtcca aaaaatagcg gtcgtgtgag
      661 acgatcaatg ctgccccgct ccactggcgc aggtaaccct ccagccactc taccgcggag
      721 atatccagat gattggtcgg ctcgtccagg atcagcaggt ctgggtcgga gagcaataaa
      781 cgggctaaga cagcgcgcgt gcgctgtccg ccagagagct gtgggatagg ccggtgaaaa
      841 tccgctggca aaaagcccag gccgctcagc gtctggcgga tgcgggtctc gtaaatgtaa
      901 cccccacgga gttcaaaatc tacctgcaag gggccatatc gctccagcgc agcctctgcc
      961 tgctcctggt cgctcatcag tccttccagg cgtgtcagct cattttccag tgacctcagc
     1021 tcttcgaagg cgcgcaggca ctcctcccac agagtatgtg tcccatgcag gatcgcttcc
     1081 tgcggcaggt aaccgacttg caaattgcgc gcccggctca acgtgccgct cgaagcctcc
     1141 tccagcccaa tcaggatacg caacagggtg gtcttgccga tcccattggg accaactaga
     1201 gcaatgcgtg ccccatgagg caggctgaga tcaatgcccg tgaatatatc ccgcgggccg
     1261 taggatttag ataggttcga cgtagtgagt agcgacatcg taatttgcta tgaatgaacg
     1321 tttaaatttc cgatggctgt tgcaaagtct atctaagtca gtctttagtg acaccatagc
     1381 ttctgttttt ctcttcccgt gaataccgtc attgcaaagg actccttcgg ggcgactgaa
     1441 gcattcttat cactcggaca ggaagattgc ttcgcctgcc agaatgcggc aggctcgcaa
     1501 tgacgcggca ttgcaatagc catttcaagc gttctgacca accgatgagg gcgattatac
     1561 cgtgttataa tgcttccctg aggctgctgc gggtattcct cgagcctatg catcgtgtac
     1621 tgtggcgcat gctaaaactc ctggttatct gcagggcttt taaatctgtg aaggaaatgc
     1681 ccagatgatc ttgaaggtct ctaaggcctt atggatctca gggttttatg gaacaaatgc
     1741 aaagaaatga caaactgatt gaacaatctg gctcaagcaa aactgtcaac cggttgaagc
     1801 tggcattgct ggcagccttt attatcgccg caggcatcac cgcctatatc acatttgtct
     1861 ttgtgcgcga tttcgtaaca tcctgggagc tgacctccct gcctgggatc accatccgcg
     1921 atgaaaagcc gacttccaca ccagatgcat cgggcgtcat ccaggatgta cagacacccc
     1981 tgcaacaagt cggaggcccc acccctgagc cctgggatgg cgccagccgc gtcacgctcc
     2041 tggtgatggg gctggattac cgcgattggg aggctggcga gggagccccg cgaaccgaca
     2101 cgatgatgct gctgactatc aacccgcttg accgcaccgc cggcattctc tcgatcccgc
     2161 gcgacctgtg ggtgaatatc ccgggcttca actacggtcg catcaacacc gcctacagcc
     2221 tgggtgaagc ctacaaatac ccagaaggag gcggtccggg actggctatg gccaccgtgg
     2281 aagagctgat cggcgtgccg gttgattatt atgcccaaat cgactttggc gctttcatcc
     2341 gcttcatcga cgagatcaac ggcatcacga tcaccatcga aaaaaggatg aaggtggatc
     2401 aaatcggtgg aaaggtcaag ttgcttcgtc cgggcacgta tacgctggat ggcgagttgg
     2461 ccttgggcta tgcccgcgcc cgcaacactg agggcggcga tttcgaccgg gccttccgcc
     2521 agcaggaagt gatcattgca atcagaaaac ggcttttaga atcggacaat ttacctaatc
     2581 tgatcgccaa gtcgccggtg ctttaccagg agctctcctc tggcgtgcat accaacctga
     2641 atctggacc
//
LOCUS       DBNHOH010000808         3717 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1462046, whole genome shotgun sequence.
ACCESSION   DBNHOH010000808 DBNHOH010000000
VERSION     DBNHOH010000808.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1462046"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /gene="murJ"
                     /locus_tag="AC16V7_14545"
     CDS             <1..1285
                     /gene="murJ"
                     /locus_tag="AC16V7_14545"
                     /inference="COORDINATES: protein motif:HMM:NF015013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HIR6258390.1"
                     /translation="PILSEFLERDGRQAAWDLFSRIVNLAFLVTACISVVIILLAEPL
                     VRHVIAPGFNDPAKWALTASLMRLDLMAILIFSISGLVMAGLQANQHFLLPAMAPGFY
                     NLGQIFGIMVLGPRLGIFGLVYGVILGALLHLAIQLPGLLRFGYRWAPAIDLRNPGVQ
                     QVLRLMGPRLLTMLCLQSYFLARDRLASFFEEGAVSALNNGWFIQQVPETLIGTAIAI
                     ALLPSLSEHFVRGQTDDFRQTVNRALRAMLALTVPIAALLAVSIRPLVRIAFDFEPRE
                     TEMVVWATRAFLLGLMGHTWLEVAVRSFYARQNATTPLLAAFLQAVAYILLAVSLSRT
                     VGHIGLALADTFSFSGQALLLLFLLNRRFPGVLQLGGTLWRAGLGALIGGGLTYALIQ
                     MLPLPILPLSLGALATGGLVVLPFIWSEVKLLVRL"
     gene            complement(1313..1504)
                     /locus_tag="AC16V7_14550"
     CDS             complement(1313..1504)
                     /locus_tag="AC16V7_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258391.1"
                     /translation="MTTLLIVLAVLIFMGIPALLFIAACIAGARASQRQEEFISYLEV
                     PASQEPVPDWMVIGEKAEA"
     gene            1673..2197
                     /locus_tag="AC16V7_14555"
     CDS             1673..2197
                     /locus_tag="AC16V7_14555"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HIR6258392.1"
                     /translation="MKLTGKHIAYLLEEGFEDLEFWVPLMRLREEGARVTIVGSGKGE
                     RFTGKHCLEAKVDVSAEQVQSADFDAVVVPGGWAPDKLRRYPAVTGLVREIYQQGKIV
                     AAICHAGLVLISAGIVKGHKSTGSLGIKDDLVNAGAIWVDEPAFRDGNLVWGRVVEDI
                     PDFCRELVKALSGE"
     gene            2230..>3717
                     /locus_tag="AC16V7_14560"
     CDS             2230..>3717
                     /locus_tag="AC16V7_14560"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR6258393.1"
                     /translation="MQKRLILFILLILILSGVPGSLAAPAPAWTSKVDPWVLQTSQDG
                     ETEFLVFLTEQADLSAAAGLKSKTEKGAYVYQTLTEVARRTQPGLLGVLRKMGIPHRS
                     YWVANMVWVRGGLSAIQSLAQRPDVARLAANPRLRLELPSVQPDVLKEGEAVEAGTWN
                     IIKINADDVWALGYTGQGAVVGGADTGYAWNHPALKRQYRGWDGISANHNYNWHDAIH
                     EGTGIPCPNNSLQPCDDNYPAHGTHTMGIMVGDDGWANKIGVAPGARWIGCRNMDDGY
                     GTPETYIECYQWFVAPTDLSGQNPRPDLAPDVINNSWGCPTTEGCNPNDLLSAVQAVR
                     AAGILTVHSAGNSGIKCGTVNEPAAIYSESFTVGATDVIDRIANFSSRGPVTVDGSNR
                     MKPNVSAPGVSIYSSLQGGSYGTLNGTSMAAPHVAGLVALLISADPSLRGQVDKLETL
                     IESSALPRLSMQDCGEDGIPNNVYGWGRVDALKAVDNLSQYFYLPL"
BASE COUNT          739 a          919 c         1150 g          909 t
ORIGIN      
        1 cccgatcttg tctgaatttc tggagcgcga cggtcgccaa gcggcctggg acctgttctc
       61 tcgcatcgtc aacctggctt ttctggtcac agcatgcatc tctgtggtca tcatcttgct
      121 ggctgagccg ctggtgcgcc atgtgatcgc gcccggtttt aatgacccag ccaagtgggc
      181 actcactgcc agtctgatgc ggcttgacct gatggcgatc ctgatcttct cgatcagcgg
      241 gttggtgatg gccggattgc aggccaacca gcatttcctg ctgccggcga tggcgccagg
      301 gttctataac ctgggccaga ttttcggtat catggtgttg ggaccccgcc tgggtatctt
      361 tggattggtc tatggagtga tcttaggggc gttgctgcac ctggcaattc aattgccggg
      421 tttgctgcgc tttggttatc gatgggcgcc tgccatcgat ctgcgtaatc cgggggtgca
      481 acaagtgctg cgcctgatgg gtccgcgcct gctgactatg ttgtgcctgc agagttattt
      541 cctggcgcgc gatcggctgg cttcattctt tgaagagggg gctgtctcgg cgctgaataa
      601 tggctggttc atccagcagg tgccggagac gttgatcggt acggccatcg ccattgccct
      661 gctgccgtcc ctctccgaac attttgtgcg cgggcagacg gatgacttcc gccagacggt
      721 aaaccgtgcc ttgagggcca tgctggcttt gaccgttcct attgctgcgc tgctggcggt
      781 gagcatccgt cccttggtgc ggatcgcttt tgactttgaa ccgcgagaga cggaaatggt
      841 ggtgtgggcg acgcgcgcct tcttgctggg gttgatgggc cacacttggc tggaggtggc
      901 agtgcgctca ttttatgcgc ggcagaacgc taccacaccc ttgctggcag cctttttgca
      961 ggcggttgcc tacatcctgc tggcagtatc gctctcgagg accgttggac acatcggtct
     1021 ggcgctggct gatacgttct cgttcagcgg acaggcattg cttttgcttt tcttgttgaa
     1081 tcgtcgcttt ccaggggttt tgcagctggg ggggactttg tggagggctg gtttaggggc
     1141 cttgattggt ggcggcctga cctatgcttt gatacaaatg ctacccttgc cgatattgcc
     1201 gctctcgctg ggggcgctgg caaccggagg gctcgtcgtg ctgcccttta tctggagtga
     1261 ggttaagttg ttagtgaggt tatagtgtta tcaggaattg cctattacaa gatcaggctt
     1321 ctgctttctc cccgatcacc atccaatctg gtactggttc ctgagaggct ggaacttcaa
     1381 gataagagat aaattcttcc tggcgctggc ttgcccttgc tccagcgatg caggcagcga
     1441 tgaaaaggag tgcaggtatt cccataaata tgagaacggc taaaactatt aataaggtag
     1501 tcatctttgt aatctcctgt agtgagatta tacatgcaaa atttatttag tgaacaaact
     1561 ttaatgcaag gataaatgga ttttatttgt tttttaacat ttcctgctat ttccggtgat
     1621 tttagaccgg tattataagg ctatttttat aaaacaggaa gatggaggga agatgaaact
     1681 gacagggaaa cacatcgcct acttgctgga agaaggattt gaggatttgg agttttgggt
     1741 gccgttaatg cgcctgcgcg aggagggcgc tcgggtgacg attgtgggca gcggcaaggg
     1801 tgagcgattc actggcaagc attgcctgga ggcgaaggtt gatgtatctg ccgaacaggt
     1861 gcagtctgct gattttgacg ccgtagtcgt gccgggcggt tgggcgccgg ataagttgcg
     1921 ccgctaccca gcagtgaccg gtctggtgcg cgagatatac cagcagggga agatcgtcgc
     1981 tgctatctgc cacgctgggt tggttttgat ctccgctggc atcgtcaagg ggcataaatc
     2041 taccggctcg ctgggtatca aagacgacct ggtcaatgcc ggggcaatat gggttgacga
     2101 gccagccttc agggatggta acctggtgtg ggggcgagtg gtggaggata tccccgactt
     2161 ttgccgcgag ctggtgaagg cattgagtgg agagtaagca gcaaggggca attgtgatgg
     2221 gttaatgata tgcagaagcg cctcattcta ttcatcctgc tcatcttgat cttatcaggt
     2281 gtcccaggtt ccttggcagc acctgcccct gcctggacaa gcaaggtgga tccctgggtg
     2341 ctgcagacct cccaagatgg ggagacggag ttcctggttt tcctgacaga acaggccgac
     2401 ctgagtgctg cggctgggtt gaagagcaag acagagaaag gtgcttacgt ttatcagacg
     2461 ctgaccgagg ttgcccggcg aacacagcct ggtttgcttg gcgtgcttcg taaaatgggc
     2521 atcccacacc gatcttattg ggtagcgaat atggtctggg tgcggggtgg tctctcggct
     2581 atccaaagcc tggcacagcg ccctgacgta gccaggctgg cagcaaatcc gcgcctccgt
     2641 ttggaactgc cttccgtcca gccggatgtg ctaaaagaag gcgaagcggt ggaagcgggc
     2701 acgtggaaca tcatcaagat caacgctgat gatgtatggg cgctgggcta cacggggcaa
     2761 ggcgcagtgg ttggcggtgc ggataccggc tacgcctgga atcatcctgc gttgaaacgc
     2821 caataccggg gttgggatgg gatttccgca aatcataact ataactggca tgatgcgatc
     2881 cacgagggga cggggattcc ctgcccgaat aactcgctcc agccctgcga cgataactac
     2941 cccgcccacg gcacgcacac catgggcatc atggtaggtg acgacggctg ggcaaacaag
     3001 ataggcgttg cgcctggagc gcgctggatc ggttgccgca acatggatga cgggtatggc
     3061 acaccagaga cgtatattga atgctaccaa tggtttgtcg ccccaacgga cttaagtggg
     3121 cagaacccgc gtccagatct ggcgcctgac gtgatcaaca attcgtgggg ctgccctacc
     3181 accgaaggtt gcaatccgaa tgatttgctg tcggctgtgc aggcggttcg ggcagccggc
     3241 atcctgaccg tccattcggc gggcaattcg ggtatcaaat gcggaacggt aaatgagcca
     3301 gccgctattt actcggaatc attcactgta ggagccaccg atgtcatcga ccgcatcgcc
     3361 aatttcagca gccgcggccc ggtgacggtt gacggcagta accgcatgaa gccgaacgtg
     3421 tctgcaccgg gtgtgagcat ctattccagc ttgcaaggag ggagttatgg aacgctgaac
     3481 ggcaccagca tggccgcgcc acatgtggct gggctggtag ccttgttgat ttcggctgat
     3541 ccatccctgc gtgggcaggt ggataagctg gaaacgctga tcgagagcag cgctctgcca
     3601 cgcctgagca tgcaggactg tggcgaggat gggataccga acaatgtcta cggttggggg
     3661 cgcgtggatg cccttaaggc agtagataat ctttcccaat atttctatct accgtta
//
LOCUS       DBNHOH010000809         2733 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_884708, whole genome shotgun sequence.
ACCESSION   DBNHOH010000809 DBNHOH010000000
VERSION     DBNHOH010000809.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_884708"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="AC16V7_14565"
     CDS             complement(<1..418)
                     /locus_tag="AC16V7_14565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6258394.1"
                     /translation="MISYHTCPLATLGGKDTGGMNVYVRDLTRQLGMLGIHVDVFTRS
                     QDEHVPHVLHDLGYGNRVVHIPAGPEVPLPKEVLASHLPEFVEGILAFAESKGLQYDL
                     IHSHYWMSGLAARDLKAEWNVPIVHMFHTLGIMKQRV"
     gene            568..640
                     /locus_tag="AC16V7_14570"
     tRNA            568..640
                     /locus_tag="AC16V7_14570"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:601..603,aa:Thr,seq:cgt)
     gene            716..1357
                     /gene="udk"
                     /locus_tag="AC16V7_14575"
     CDS             716..1357
                     /gene="udk"
                     /locus_tag="AC16V7_14575"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HIR6258395.1"
                     /translation="MQNRATPVVIGIAGGTGSGKTTVANVIMDRVGARHIAYLPHDAY
                     YKDLSALPEAQRVMVNFDHPNSLDTELLIFHIRQLRDWQTVDLPVYDFKTHSRTKEII
                     QIKPQPVILVEGILIFAEAALRPLFDVKIFVDTNADIRFIRRLQRDIEERGRTTHSVI
                     QQYLNTVRPMHLEFVEPSKRYADVIIPEGGLNTVAMDMVVARLEALLRGNNIG"
     gene            1354..1875
                     /locus_tag="AC16V7_14580"
     CDS             1354..1875
                     /locus_tag="AC16V7_14580"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HIR6258396.1"
                     /translation="MKNLRINIARVVAALLVIAISVTIYSIRDRADQFAVYGYPGIFF
                     LAFLAYATVLLPAPGLAVIFTLGAVFNPVGVALAAGSGAALGELTGYMAGFSGQAIVE
                     RTKIYARIAGWMKYNGPLTILFLAALPNPFFDIVGAIAGISKMPLLKFLFWCWIGETL
                     KMLFFALIGSGIL"
     gene            1893..>2733
                     /locus_tag="AC16V7_14585"
     CDS             1893..>2733
                     /locus_tag="AC16V7_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR6258397.1"
                     /translation="MMKNFEGIDTYLESNLEQSIQELSLLAAQPSISAQNLGLEECAH
                     LVGDLLKKRGFTVEIMQTGGPPVVYAERRGQSDKTLLFYNHYDVQPPEPLELWESPPF
                     EPSLRDGKLYARGVSDDKGHITSRLFALDALIDSNGDLPCNVKFVIEGEEETSSVHLN
                     KFVKDNAEKLAADACVWEFGQVDHREVPIQHLGLRGICYVELSVETAEQDVHSGLGGS
                     IFPNAAWRLVWALNTIKGPDERILIPDHYDNVVAPSEQDEQYIAELPEVADEYKNRYG
                     VQYF"
BASE COUNT          676 a          703 c          663 g          691 t
ORIGIN      
        1 ccacccgttg cttcattatg ccaagcgtgt gaaacatgtg cacgatggga acattccact
       61 ccgccttcag gtctcgggct gccagccccg acatccaata atggctgtgt attaagtcgt
      121 actgcaatcc cttgctttct gcgaaagcta gaataccttc cacaaactca ggaagatgag
      181 atgccagaac ttctttgggt agcggtacct ccggacctgc cggtatgtgt accacccggt
      241 taccataacc caggtcgtgc aaaacatgcg gcacgtgctc atcctgcgaa cgcgtaaaga
      301 catccacatg gatacccaac attccaagct gacgagtcaa atcgcgcaca tacacattca
      361 taccgccagt gtcttttccg cccagggtgg ctagcggaca agtatgataa gatatcatgg
      421 ctatgcgcaa ctcacacctc gtcttacgcg actgccttgc aattcctacc tatcccttgt
      481 ataatgtgcc ccgcatccca aattttacga cccgcgcctc ccaaacctac caggaggtca
      541 gcaaccttga tgggttcata atcaggtgcc accgtagctc agttggcaga gcaggcgttt
      601 cgtaaacgtc aggtcaaggg ttcgaatccc ttcggtggct ctctttaatt gggatgaatg
      661 agtgggtata cctctcattc atcccaaatt tattccattc cagtataatt ccagtatgca
      721 aaatcgtgca accccagttg tcattggaat cgcgggaggt actggttccg gtaaaacaac
      781 ggtggcgaat gtcatcatgg atagggttgg cgcacgccat atcgcctacc tgccgcatga
      841 tgcgtattat aaagacctca gcgcacttcc cgaggcgcaa cgcgtcatgg tgaattttga
      901 tcaccccaac tcattggata ccgaattact catcttccat atccgtcagc ttagggactg
      961 gcaaaccgtc gatttgccgg tttacgattt taagacccac agccgaacca aggaaatcat
     1021 ccagatcaag cctcaaccgg tcatactggt cgaaggtatc ctgatttttg ccgaggcagc
     1081 cctgcgcccg ctcttcgatg tcaagatttt tgtggatacc aatgccgaca tccggttcat
     1141 tcgccgcttg caacgtgata ttgaggagcg cggccggact actcactcag ttatccagca
     1201 ataccttaat accgtgcgcc ctatgcacct agaattcgta gaaccatcca aacgttatgc
     1261 tgatgtgatt atcccagagg gtggattgaa caccgtggct atggacatgg ttgtggcgcg
     1321 cttggaagcc ttattgcggg gtaataacat cgggtgaaaa atcttcgcat caacatcgcg
     1381 cgcgtcgttg cagcactgct cgtcattgct atcagtgtca ccatatatag cattcgtgac
     1441 cgggcagacc agttcgctgt ctatgggtat ccgggcatat tttttcttgc ctttttggca
     1501 tacgctactg tactactacc cgctccaggc ttagcagtta tcttcacctt gggcgcggtg
     1561 tttaatcccg ttggtgtcgc cctggcagct ggcagcggtg cagcattggg agagctaact
     1621 ggctacatgg caggttttag tggacaggct atcgtcgagc gcaccaagat ttacgcccgg
     1681 attgcaggct ggatgaaata taatggtcct cttacaatcc tttttctggc tgctttgcct
     1741 aatccctttt ttgatattgt cggcgcgatc gccggaatct caaaaatgcc cctgctaaaa
     1801 tttttattct ggtgctggat tggtgagacg ctcaaaatgt tattttttgc cctaattgga
     1861 tcgggcattc tatgatcttt cactaaggaa aaatgatgaa aaattttgaa ggaattgaca
     1921 catacttaga atcaaattta gagcaaagca ttcaggaatt atcattgctc gccgcacaac
     1981 caagcatcag tgcccaaaac ctggggttgg aggaatgtgc ccatttggtt ggggatttac
     2041 tcaaaaagcg aggctttaca gtagagatca tgcagaccgg aggcccacct gtagtttacg
     2101 ctgaacggcg cgggcaaagc gataaaacat tgctgttcta caaccattac gatgtccaac
     2161 cccctgagcc gctcgagctt tgggagagcc caccattcga acccagcttg cgtgatggga
     2221 agctctatgc tcgcggggtt agcgatgata aaggtcacat caccagccgt cttttcgcgt
     2281 tggatgcctt gatagactca aatggtgatc tgccctgcaa cgtcaaattc gtaatcgagg
     2341 gtgaagaaga gaccagcagc gtccatctca ataaatttgt caaggacaac gcagaaaagc
     2401 tcgccgcaga tgcttgtgta tgggagttcg ggcaggtgga ccatcgcgaa gtgccaatcc
     2461 agcaccttgg cctgcgcgga atttgctatg ttgagttatc tgtggagaca gcggagcagg
     2521 atgtccactc gggacttggc ggatcaattt tcccgaatgc agcctggagg ctggtttggg
     2581 cattgaacac cataaaaggg ccagacgagc gcattctcat ccctgatcac tacgacaatg
     2641 ttgttgcgcc tagcgagcaa gacgagcagt acatagcaga actccctgaa gtggcagacg
     2701 aatacaagaa tcgttatgga gtacaatatt ttc
//
LOCUS       DBNHOH010000810         6415 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_435610, whole genome shotgun sequence.
ACCESSION   DBNHOH010000810 DBNHOH010000000
VERSION     DBNHOH010000810.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6415)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6415)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6415
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_435610"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="AC16V7_14590"
     CDS             complement(<1..182)
                     /locus_tag="AC16V7_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C assembly protein"
                     /protein_id="HIR6258398.1"
                     /translation="MGSKPTLLKILDVVTVILLVVATGMVFFYAPMEAVMGQVQRVFY
                     FHVAAGWVGMLSFLVAA"
     gene            complement(287..1015)
                     /locus_tag="AC16V7_14595"
     CDS             complement(287..1015)
                     /locus_tag="AC16V7_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA];
                     GO_process: GO:1903607 - cytochrome c biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1904334 - heme import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HIR6258399.1"
                     /translation="MSDTPQVMAHKDPLEGRAAFSFLRAMGAVVWKDLAAELRSRELL
                     SSMLVFALLVILIFNFALELDARERANVTSGVLWVTFTFAGTLGLNRSMAIEKDRGCL
                     DGLLLAPVDRSAIYFGKTLGNLIFMLLVEIIVLPIYSVLYNTNLFLPGLLLVILLGSV
                     GYVAVGTLLASMAVQTRTRDILLPILLFPVVIPVVIASVKGSGGYLQGIPFDEIMPWI
                     NLLIVYDVIFTAVAFMVFDYVVEE"
     gene            complement(1012..1719)
                     /gene="ccmA"
                     /locus_tag="AC16V7_14600"
     CDS             complement(1012..1719)
                     /gene="ccmA"
                     /locus_tag="AC16V7_14600"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HIR6258400.1"
                     /translation="MIEVRRLVKRFGLKAVLRGLEFHVEPGEFVALLGPNGAGKTTFL
                     RILASLSRPTLGEVRIAGYRLPAQASAVRRRLGVVSHLPLLYGDLTAEENLQFYGRMY
                     GINDLAMRIEEVLNLVGLWPRRRDLVRTFSRGMQQRLAIGRAVLHDPDVMLFDEPHTG
                     LDQDASSMLDNVLGEVAARGRTVVMTSHDLSRAADLASRFDVLSRGVITASVKREEID
                     TRDLLAFYRQAVNRGTA"
     gene            complement(1751..3706)
                     /locus_tag="AC16V7_14605"
     CDS             complement(1751..3706)
                     /locus_tag="AC16V7_14605"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6258401.1"
                     /translation="MHRQSQPRRLRPITSRFVLLPFALLLFSLIISACSFSLAADITP
                     PPGSEQRPPIAVTQPAPISGPLYPLVPPNLESGKAIFEEKCAPCHGDTGLGDGPQSAQ
                     LPNPVAAIGSIELARQSTPARWYTQVTQGNLQKFMPPFNSLSDRQRWDVVAYSFSLTT
                     PPELVDQGQELYQANCARCHGGTGKGDGQDAAGGPKPPTNLTDQAFMAEKSADDFFQV
                     TKNGLPPAMPAFGAQLSEDEIWALAAYLRSLTFTAPDEPVAVAETPTPGEAATPTSAS
                     ATPIAEVPAGIGTVTGKVISAAGVELPEEMEILLHGFDNMQVAMTQTTTTQTDGSFIF
                     PDIQMPDGRVFLATVNYGRTSYGSDIGIAQDGINILDLPIVVYETTSDASILKADRLH
                     LFFEFVDAKTVRVIELYIVSNPSDKSLVAPEPGEASVVFSLPDGATKLEFQDGALGGR
                     YIKTADGFGDTVSIPPGFGSYELLFAFEMPYDRNLELAHPVTMPVDAIVILAPEGDIK
                     IKSDILQDSGVRDVQGTQYRTYSGGGLAAGDELRLTLTGKPSIGAPSLTTSSNTSLLV
                     GLSVFGLALIMAGVWLYRRTRLNGADSLDAEQALDSSDSADFETSDSLMDAIIALDDL
                     YQAGQLPDEAYQQRRDELKRRLKELIG"
     gene            complement(3737..4261)
                     /locus_tag="AC16V7_14610"
     CDS             complement(3737..4261)
                     /locus_tag="AC16V7_14610"
                     /inference="COORDINATES: protein motif:HMM:NF024637.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR6258402.1"
                     /translation="MDIGSIFLILGLLVLVGLFIGRPLLEHQATVVSQEEHQYSSLLA
                     ERDRILNALQELDFDYTLGKIPEADYPGQRASLLTRGADILRQLDAFGVETSEEEAEA
                     QLEAVIAARRADIASNGGPGRILRTSPDDDLEALIASRRRVRSEKARGFCPQCGRPVQ
                     TSDRFCPNCGATIA"
     gene            complement(4340..4909)
                     /locus_tag="AC16V7_14615"
     CDS             complement(4340..4909)
                     /locus_tag="AC16V7_14615"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR6258403.1"
                     /translation="MEQSESLVTQAPERTRTQWGRVLVWVGLMGLLVILALGLKRSQQ
                     GPVSVGDRVPNFTLTTFDGAQINLEDLQGKVVVLNFWASWCKPCEQEAADLEAAWRFY
                     KPRGDVVFLGVDYVDTEPEALAYLKKFDITYSNGPDLGTRISQAFRIQGVPETYIIDQ
                     QNVLRFVQIGPFTSLEQIKAAIDPLLTGG"
     gene            complement(4911..5390)
                     /locus_tag="AC16V7_14620"
     CDS             complement(4911..5390)
                     /locus_tag="AC16V7_14620"
                     /inference="COORDINATES: protein motif:HMM:NF015853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HIR6258404.1"
                     /translation="MRRFISLLALIFAFVLSGSALAQDGSPVAPSDDQVNAIAKQLYC
                     PVCENIPLDVCGTQACAQWRELIREKLSQGWSEPQIKQYFVDQYGDRVLATPPARGLN
                     WLVYLVPPLAIAAGVYILYRALKAWKQPTPLEDSAASSSSTQTDEYVRRLEEELKKR"
     gene            complement(5436..>6415)
                     /locus_tag="AC16V7_14625"
     CDS             complement(5436..>6415)
                     /locus_tag="AC16V7_14625"
                     /inference="COORDINATES: protein motif:HMM:NF027651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HIR6258405.1"
                     /translation="TSMLSREALFLLNNLLFIGILVVCFWGVIFPLISELATGQKVTV
                     GPPFYERATGPLFAGLLLLMGIAPLAAWRHSTANTLGRAIWKPGLFSLLVLAIVIIGG
                     VRHVAALIGFWLSAFVASVTLYEFWRGALARHQKSGESLPLALWRLAGRNRRRYGGYV
                     IHLGVVLMAIGIIGIEIFQTETQGTIAQGERISLGNYSITFESLATFDTPDGRNVARS
                     VVSVYKDNQFVGELYPRRDYYYESQQPMTIPGVRSTWEDDFYVLLVDWQPVSTAGATF
                     KVYHNPLVNWLWLGGFVFILGTLLAAWPDQDPMPETRRARRAEYASAKA"
BASE COUNT         1506 a         1756 c         1821 g         1332 t
ORIGIN      
        1 gcagccacca gaaagctaag catacctacc cagccagccg caacgtggaa ataaaaaact
       61 cgctgcactt ggcccatcac cgcttccatg ggagcgtaga agaaaaccat gccggtggca
      121 acgaccagca ggataacggt gacaacatcc agaattttca ataaagttgg tttgctaccc
      181 ataaaatcct ccttgttttc ggaatcagag agtctagcat cgagattaat tgccttgcga
      241 tagacgaatc atgtatctcg aatccctact ctcataactc taatctctat tcttccacca
      301 cataatcgaa caccataaat gctacggcag taaagatgac atcataaacg atgagcaggt
      361 tgatccaggg catgatttcg tcgaagggga tgccttgcaa gtatcctccg ctgcccttga
      421 ctgacgcaat cacgaccggg atcacaaccg ggaatagcag gatcggtagc aggatgtcgc
      481 gggtacgtgt ctgcactgcc atgctcgcca gcaacgtgcc caccgccaca tagcctaccg
      541 agcccaacaa gatgaccagc aacaagcctg gcaggaacaa attcgtatta tacagcacgc
      601 tgtagatcgg cagcacaata atttcaacca acagcatgaa gatgaggttg cccagtgttt
      661 tcccgaagta gatggcgctg cgatctaccg gtgccaggag caggccatcc aggcagccgc
      721 ggtctttttc gattgccatg gagcggttca agcccagcgt gccagcaaat gtgaaggtca
      781 cccacagcac accagaggtg acgttggcgc gctctcgggc atccagctcc agggcgaagt
      841 taaagatcaa aattaccaag agagcaaaga caagcatgga ggagagcagc tctcggctgc
      901 gcaattcggc agccaggtct ttccatacca cggctcccat ggcgcgcaga aagctaaaag
      961 cagctctacc ctctagcggg tccttgtggg ccatcacctg gggggtatcg ctcatgccgt
     1021 tcccctgttg accgcctggc ggtagaaagc cagcaagtct cgcgtatcaa tttcttcacg
     1081 ctttaccgaa gcagtgatca caccccgcga aagcacgtcg aagcgggatg ccaggtcggc
     1141 agcccgggag aggtcgtggg aggtcatcac gactgtgcgg ccacgagcag cgacctcccc
     1201 caggacgtta tccagcatgc tgcttgcgtc ttgatccaac ccggtgtgtg gctcatcgaa
     1261 tagcatcaca tccgggtcgt gtaaaacagc tcgcccgatt gccaggcgct gctgcatgcc
     1321 gcgagagaag gtgcgcacca ggtctcggcg gcgaggccat aggccgacca ggttcagcac
     1381 ttcttcgatg cgcatagcca gatcatttat gccgtacatg cgcccgtaaa attgcaggtt
     1441 ctcttcagcc gtcagatcgc catacaagag gggcaggtga gagacgacac ccaggcggcg
     1501 gcgtactgca ctggcttgag caggcagacg atacccggcg atgcgcactt ctccaagggt
     1561 gggccgcgat aatgaagcca gaatgcgcaa aaaagtggtc ttgccagccc cattcgggcc
     1621 gagcaatgcc acaaattctc cgggctctac atgaaattcc agcccgcgca atacggcctt
     1681 caggccaaag cgcttgacga ggcggcgtac ttcgatcatg ggaattgagg gatggggtca
     1741 ggggtatccc ttacccaatg agctccttca accttctctt caattcgtct cgccgttgtt
     1801 ggtaggcttc atcaggtagc tgaccggcct ggtagaggtc atccagtgcg atgatggcat
     1861 ccatgagact atcgctggtc tcaaagtcgg ccgaatccga agaatccaaa gcttgctcag
     1921 catccagcga atcggcgccg ttaaggcgcg tgcgtcgata cagccacaca ccagccatga
     1981 tgagcgccaa accgaaaaca gacagcccaa ccagcaggct tgtgtttgag ctggtggtca
     2041 gggagggagc accaatggag ggtttcccgg tcagggtgag tctcaactcg tctcccgcgg
     2101 ctagcccgcc tccactgtaa gtgcggtatt gtgtaccttg tacgtcgcga acgccactgt
     2161 cctgcaatat gtcgctcttg atcttgatgt cgccttcggg cgcaaggatc acgatggcat
     2221 ccacgggcat ggtcaccggg tgagccagtt ccaggttgcg gtcatagggc atctcaaaag
     2281 cgaacaacag ctcataagat ccaaaacctg gagggatgga gacagtatca ccgaaaccgt
     2341 ctgcggtttt aatatatcgc ccgcctaacg ctccatcctg gaattccagt ttcgttgctc
     2401 catctgggag ggaaaagacc acagacgctt ctcctggctc aggggctacc agggatttat
     2461 cgctggggtt ggacacaatg tagagctcga taacccgcac ggtttttgcg tcaacaaact
     2521 cgaagaacag gtgcagtcgg tcggctttta atattgaggc atcgctggtg gtctcgtata
     2581 cgacaatagg cagatccaag atgttgatgc catcttgggc gatgccaata tcggaaccat
     2641 aggaggtgcg cccataattc actgtagcca gaaagacacg cccgtcaggc atctgtatgt
     2701 cagggaagat aaatgaaccg tctgtttgtg tagtggtggt ctgggtcatt gctacctgca
     2761 tattgtcaaa gccatgcagt aggatttcca tttcttcagg cagctcgact ccagcggcgc
     2821 tgatcacctt gcctgtcact gtgccaatgc cagcaggcac ttccgcaatc ggagtggcag
     2881 acgcagaagt gggtgtggct gcttctcccg gagtgggagt ctcagctact gcgaccggct
     2941 catctggtgc ggtaaaggtt agcgatcgta aataagccgc cagggcccag atttcatcct
     3001 cggaaagctg cgcgccgaag gccggcatgg ccgggggcag gccatttttg gttacctgga
     3061 agaagtcgtc agccgatttc tctgccatga atgcttgatc ggtcaaattg gttggcggtt
     3121 taggaccccc agctgcatct tgcccatcgc ctttacctgt ccccccatgg catctagcgc
     3181 agttggcctg gtaaagctcc tgaccttgat cgaccagttc agggggtgtg gtcagggaaa
     3241 aagaataggc caccacatcc caacgctggc gatcggatag gctattaaat ggcggcatga
     3301 acttctgcaa gttgccctga gttacctggg tataccagcg ggccggcgtg gactggcgag
     3361 ctaactcaat cgagccgatg gcggctaccg ggttaggaag ctgagctgac tggggcccgt
     3421 ccccaagacc tgtatcaccg tggcaggggg cgcatttctc ctcaaaaatg gctttgccgc
     3481 tctctagatt aggtggcacc agcgggtaaa ggggaccact gatcggcgca ggttgtgtca
     3541 cagcgatggg tggtctctgc tctgagcctg gtggcggagt gatatcggct gccagggaga
     3601 aggagcaagc cgagatgatc agagagaaca gcaatagtgc aaatggaaga agaacaaaac
     3661 gagaagttat tgggcgtagc cggcgaggtt gagattgtct gtgcatatta aatctatgat
     3721 caccgataat ggggtactag gctattgtag ccccgcaatt ggggcagaaa cgatcggagg
     3781 tttgtaccgg acgcccacac tgcgggcaga agccccgggc tttctcactg cgcacgcggc
     3841 gccggctggc aatcagggct tccagatcgt catccggcga ggttcgcagg atgcgaccag
     3901 ggccaccatt agaggcgata tcggcgcggc gcgcggcgat aacagcctcc agctgggcct
     3961 cggcctcctc ttcgctagtc tccaccccaa acgcatctag ctggcgcagg atatccgcgc
     4021 cgcgagttag tagggaggct cgctgcccag ggtaatccgc ttcggggatt ttgccaagcg
     4081 tgtagtcaaa atccagctct tgtagggcgt tcaggatgcg gtcgcgttcg gcgagtaagc
     4141 tggaatattg gtgctcttcc tggctaacaa ccgtggcttg gtgctccagg agaggacgac
     4201 cgatgaagag tccgaccagg acgagtaaac caaggatgag aaagattgag ccgatgtcca
     4261 ttttacagtg tttagagatt agggatcatt gggttgcgag ttgtagtttg tggtgtacac
     4321 ggttcaagca aactgctcac taccctccag ttaatagcgg atcgatggca gctttgatct
     4381 gctccagaga agtgaacggg cctatctgca caaagcgtag cacgttttgc tgatcgatga
     4441 tgtaggtctc tggcacgccc tggatgcgaa aagcctgaga gatgcgcgtg cccaggtctg
     4501 gcccgttgct gtaggtaatg tcgaacttct ttaggtaagc aagggcttca ggctcggtgt
     4561 ccacataatc tacgcccagg aagaccacat cgccgcgtgg cttgtaaaag cgccaagcag
     4621 cctccaaatc ggcggcttcc tgctcgcagg gcttgcacca tgaagcccag aaattgagca
     4681 cgactacctt gccttgcagg tcttccaggt tgatctgggc gccatcaaaa gtggtcaggg
     4741 taaaattggg gacccggtcg cctactgaca ctggcccttg ctgagatctc ttcaaaccca
     4801 gggcaaggat aaccagcaag cccatcaacc ctacccacac aagcacacgc ccccactggg
     4861 tgcgtgtgcg ctcaggtgcc tgtgttacta ggctttctga ttgttccata ttaccttttc
     4921 ttcaactctt cttctaagcg ccggacgtat tcgtccgttt gcgtgctgga tgatgatgcg
     4981 gcggaatcct cgaggggggt tggctgcttc caggctttaa gagctcgata gaggatgtac
     5041 acgccagctg cgatagcaag cggcggaacc aggtagacaa gccaatttag acctcgcgcc
     5101 gggggagtag ccaagactcg gtcgccgtac tggtctacga aatattgctt gatctgtggc
     5161 tcgctccacc cttggctgag cttttcacga atcaattctc gccattgggc gcaggcttga
     5221 gtgccgcaaa catccaatgg aatattttcg cacaccggac aatatagctg tttggcgatg
     5281 gcgttgactt gatcgtcgct aggcgctaca gggctgccgt cttgtgctaa agcggaaccg
     5341 ctgagaacga aggcgaagat taatgcaagc agtgatataa agcgtctcat gttggtttcc
     5401 gagttatggg tcattaatca gttacatatc atgcctcagg cttttgctga ggcatactcg
     5461 gcccgcctcg cccgccgcgt ctctggcatg ggatcttgat caggccaggc ggctagcagc
     5521 gtgccgagga tgaatacaaa cccgccaagc cacagccagt tgaccagcgg gttatggtag
     5581 accttaaagg tcgctccagc ggtagatacc ggctgccagt ccaccaggag cacatagaaa
     5641 tcgtcctccc aggtgctgcg cacgccgggt atcgtcatgg gttgctgcga ttcgtagtag
     5701 taatcccggc ggggataaag ctctccaacg aattggttgt ccttgtacac actgaccaca
     5761 gagcgagcca cattgcgccc gtcgggggta tcgaaggttg ccagggattc aaacgtgatg
     5821 gaataattcc ctaatgaaat gcgctcgcct tgagcgatgg tgccctgggt ctcagtctgg
     5881 aatatctcga tgccgataat gccgatcgcc atcagcacca cccccaggtg gatgacatac
     5941 ccaccatagc ggcggcgatt acgtccagcc agacgccaaa gcgccagcgg taaactctca
     6001 cccgacttct ggtggcgagc cagcgcgccg cgccagaact cgtagagcgt cacggaagcc
     6061 acaaaagctg atagccagaa cccgatcaag gctgccacgt ggcgcacccc cccaataatg
     6121 actattgcca gaaccagcag ggagaaaagt cccggcttcc agatggcgcg ccccaaggtg
     6181 ttagcagtgg agtgccgcca ggcagccaga ggtgcaatgc ccatcagcag caacagacct
     6241 gcgaagagag gcccagtagc ccgctcgtag aagggtggtc caacggtcac cttctgaccg
     6301 gtagccagct cggagatgag cgggaagatc acaccccaaa agcagacgac caggatgccg
     6361 atgaagagca ggttattgag caggaagagc gcctcgcgtg agagcatgga cgtca
//
LOCUS       DBNHOH010000811         2079 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_628565, whole genome shotgun sequence.
ACCESSION   DBNHOH010000811 DBNHOH010000000
VERSION     DBNHOH010000811.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_628565"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            273..1928
                     /locus_tag="AC16V7_14630"
     CDS             273..1928
                     /locus_tag="AC16V7_14630"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA mutase large subunit family protein"
                     /protein_id="HIR6258406.1"
                     /translation="MSLESEQARWEEQTLKPGLKRTAERKGEFHTPSGIPVPRVLTPP
                     DPDPDYSKKLGFPGEHPFTRGVQPTMYRGRLWTMRQYAGYATAEESNRRYRSLLDQGQ
                     TGLSVAFDLPTQIGYDADDPIARGEVGKVGVSISSLDDMETLFSKIPLDKVSTSMTIN
                     APASILLAMYIAVAKRQGVEPRQLRGTIQNDIFKEYIARGTYIFPPQPSLRLITDVFR
                     YCQVDVPLWNTISISGYHIREAGSTAVQEVAFTLANGIAYVQAAVEAGLDVDGFAGQL
                     SFFFNAHNNFLEEVAKFRAARRMWARIMRERFGAKKPSSEMLRFHTQTGGSTLTAQQP
                     ENNVVRVTLQALAAVLGGTQSLHTNSMDEALWLPTERAVRVALRTQQIIAHESGVADS
                     VDPLAGSYLVEQLTDEIEQRASKYIREIDEMGGALAAIEKGYMQTEIQESAYRYQQAV
                     EAGDEVVVGVNAFQVEEHLELERLKVDPAIEEAQRARLAALRSRRDATRTSELLSHLE
                     DAAKSSQNLMPLFIECVENDLTLGEICRLLRRLWGEYHPPAWL"
BASE COUNT          429 a          619 c          596 g          435 t
ORIGIN      
        1 ggttttaggg ttgcgcatag tggtctccta ttaattgacc ttcagatagc ttggttatga
       61 acgtcccgca ggttcgtgct cacacaggtt tgttttaggg aaacctgcgg gacgttttct
      121 attgtacatc ctttgcgccc gcttgcagac gaaatttatt tagctggcag gtgtaatgtt
      181 ctctaatctt atcctcttag ggtatacttc tctagccgac caaatgctga aggctgatct
      241 ctgattacaa tccatttatc cctgaggtcg tcatgtcctt ggaatccgaa caagcacgct
      301 gggaagagca aactctcaag cctggcctca agcgcacagc cgagcgcaag ggggagttcc
      361 acaccccttc cggcatccct gtgccccgtg tactgacccc gccagaccca gaccctgatt
      421 attccaaaaa gcttggcttc cccggcgagc accctttcac gcgcggcgtg cagcccacta
      481 tgtaccgcgg ccgcttgtgg acaatgcgcc agtacgccgg ttacgccact gcagaagagt
      541 caaaccggcg ctaccgttcc ctgcttgacc agggacagac cggcctctca gtggcctttg
      601 acctgcccac acagatcggc tacgacgctg atgaccccat cgcgcgcggc gaggtgggca
      661 aggtgggcgt gtccatatcg tccctggatg acatggagac cctgtttagc aaaatccccc
      721 tggacaaggt ctctacgtcg atgacgatca acgccccggc gtcgatcttg ctggcgatgt
      781 acatcgctgt ggccaagcgc cagggggtag agccgcgcca gctacgcggc accatccaga
      841 atgacatctt caaagagtat atcgcccgcg gcacctatat cttcccgcca cagccatccc
      901 tgcgcttgat cacggatgtg ttccgctatt gccaggtgga tgtgcccctg tggaacacca
      961 tcagcatctc cggttaccac atccgcgagg ccggctccac ggcggtgcag gaggtggctt
     1021 ttaccctggc gaacggcatc gcttacgtgc aggcggctgt ggaggctgga ttagatgtag
     1081 acggctttgc cgggcaactc tcgtttttct ttaatgccca caacaacttc ctggaagagg
     1141 tcgccaagtt ccgcgccgcc cgccgcatgt gggcgcgcat catgcgcgag cgctttggcg
     1201 ccaagaagcc atcctcggag atgctgcgct tccacaccca gactggcggc tccacgctca
     1261 ccgcacagca gcccgagaac aacgtggtgc gcgtcacctt gcaggcgctg gcggcggtgt
     1321 tgggcggcac ccagtcgctg cacaccaaca gcatggacga ggccttgtgg ctgccaaccg
     1381 aacgcgccgt gcgcgtggct ttgcgcacgc agcagattat tgcccatgaa tcgggcgtgg
     1441 cggacagcgt cgacccgctg gcgggctcct acctggtcga gcagctgacg gatgagatcg
     1501 agcagcgcgc cagcaaatat atccgtgaga tcgatgagat gggcggtgcg ctggctgcta
     1561 ttgagaaggg ttacatgcag accgagatac aggagtcagc ctaccgctat cagcaggcag
     1621 tggaagcggg tgacgaggtc gttgtgggtg tcaatgcctt ccaggtagaa gaacacctgg
     1681 agttggagcg cctgaaagtg gacccagcca tcgaagaggc gcaacgcgcc cgcctggctg
     1741 cccttcgcag ccggagagac gctacacgca ccagtgagct actcagccat ctggaggacg
     1801 ccgccaaaag cagccagaat ctgatgccgc tgttcatcga gtgcgttgaa aatgatctga
     1861 ccctgggtga gatttgcaga ctgttgcgcc gcttgtgggg cgagtaccat cctcccgcct
     1921 ggttataatt agggggtcgc ttgaagtttg aaagtcacca cttaatcacc gccgcagctt
     1981 tcatcgggag agaacatgcc caagattaaa cgcattgacc atatcgccat cgtcgttgaa
     2041 gatatcgaag ccgccctggg cttttggcgt gataacctg
//
LOCUS       DBNHOH010000812         2664 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_949714, whole genome shotgun sequence.
ACCESSION   DBNHOH010000812 DBNHOH010000000
VERSION     DBNHOH010000812.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_949714"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="AC16V7_14635"
     CDS             complement(<1..505)
                     /locus_tag="AC16V7_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR6258407.1"
                     /translation="MEKNEYLDLYYQMVLIRIVEEQAAQLYQQGKIGGFLHLYIGQEA
                     VSTGLISVRQPQDRVITAYRDHGVAINCGISAKEVMAELLGKATGISKGKGGSMHMAD
                     VKKNFWGGHAIVGAHLPLAAGLALGDQYQGNDGVTICMFGDGATNIGYFHEALNLSKV
                     WNLPVLWV"
     gene            951..1658
                     /locus_tag="AC16V7_14640"
     CDS             951..1658
                     /locus_tag="AC16V7_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR6258408.1"
                     /translation="MKSKRFPLLLVTILLVIFISACERPASVAPESVGSPTVPEGGFP
                     LPGVTEDVMGQLESFATQTAFALAGGVTAPETPTGEVAPAATSTPEAAAAAAPTATAT
                     TAPQAASSSSCSSIVIPTATPGQPSSWTLQRGEHPYCIARRFDVNPVDLLNASGLNSG
                     SSYAVGTKLTIPKSARGFPGKRSLKSHPDTYTVGSRDSIYSIACEYGDADPYAIALAN
                     GLSAPYKLTNGQQLCIP"
     gene            1747..2283
                     /locus_tag="AC16V7_14645"
     CDS             1747..2283
                     /locus_tag="AC16V7_14645"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR6258409.1"
                     /translation="MMTFQKISSSRIYRGRVFDLHQDEVRLPNGRVVQLDIVQHPGAV
                     VLVPVDAQGNIWFVRQYRHAAGTEMLELPAGTMERGEDIESCAYRELREEIGMAAGEV
                     LKIGEFFMAPGYSTEYVYIYLATSLQPAPLPGDDDEFIHVERMAAGEAFELAEKGQIP
                     DAKTVAALSLARPHIQRF"
     gene            complement(2295..2576)
                     /locus_tag="AC16V7_14650"
     CDS             complement(2295..2576)
                     /locus_tag="AC16V7_14650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HIR6258410.1"
                     /translation="MAGEDTNETNKFVALCPECQTGVLRLRSLTYFTWLNTELITVPN
                     FPAWVCDVCGRREYDPRAIKWLNTLLNPEAGRSQNRPRSMPPRVDRPRP"
BASE COUNT          669 a          700 c          667 g          628 t
ORIGIN      
        1 acacccacag cacaggcaga ttccaaactt ttgaaaggtt gagcgcttcg tggaagtaac
       61 cgatgtttgt ggcaccgtcg ccaaacatac agatagtcac gccatcatta ccctgatatt
      121 gatcgcctaa agccaaccct gctgccaggg ggagatgagc tcccacgatc gcatgaccac
      181 cccagaaatt tttcttgaca tcggccatat gcatagagcc gcccttacct ttcgagatgc
      241 cggtggcttt gcctagcagt tctgccatga cttccttggc tgaaatgccg caattaatcg
      301 ccacaccgtg atcgcggtaa gccgtaataa ctcgatcttg tggctggcgc acggaaatca
      361 ggcctgtgct gacagcctcc tgaccgatat ataggtgtaa aaagcccccg atttttccct
      421 gctggtagag ttgggcagct tgttcctcga cgatgcggat cagcaccatc tggtaataca
      481 aatcgagata ctcgttcttc tccatatttt ctcccacata agaacaccgg ggaaaccccg
      541 gcgttaaaag tagataccat taagtcgtcg taccagcaaa aattatacca gaatcctcca
      601 agataggagt aataagctcg acgatcagta gggcaatcgc attaggacga tttcccacca
      661 ctgaagagag tgtttggggg attgcgcatg cccctcaggt catatgcaat tgccctgggt
      721 ctctttgggt agagcatggt gagggcaaac gatggatggg acataaaaaa tatcaatatg
      781 tcgcttaaag acagtttcgc aagtatttat gcaagaatct tgacgcttta ccatgattgt
      841 gctagtatca ttgcaaagtt cgagagaccg ctgcggtttt cgaacaaaat actcacgcat
      901 ggttgcctgc aatcaggcca agtgggtgaa aacagttaag gagagaaaag atgaaatcca
      961 aacgctttcc gctgctcctg gtgacaattt tattggttat tttcatatct gcatgtgaac
     1021 gaccagcttc tgttgctccc gaaagcgttg gctcaccaac ggtgcccgaa ggcgggttcc
     1081 ccctacctgg cgtcactgag gacgtgatgg gccaattgga gagctttgct acccagacag
     1141 cctttgccct ggctggcgga gtcaccgccc cagagacgcc cacaggcgaa gttgcacctg
     1201 ccgcaacgtc taccccagaa gcggcagcgg cagctgctcc cacagccaca gcaaccactg
     1261 ctccccaagc tgcttcatca tcttcttgta gttcgattgt aattcccact gcgacaccag
     1321 gccaaccatc ctcctggaca ctgcagcgtg gtgagcatcc gtattgtatt gcccgccgct
     1381 tcgatgtcaa tccggttgac ttgctaaacg caagcggatt gaattcaggt agttcctacg
     1441 cggtgggcac gaaacttacc attcctaaaa gtgcgcgtgg cttcccgggc aaacgctcct
     1501 tgaagtccca tcccgatacc tacacggtgg gatcgaggga ttcaatctat tccatcgcct
     1561 gcgagtatgg cgatgcagac ccatatgcta ttgccttagc aaacgggttg agcgcaccct
     1621 acaagctcac gaacggtcaa cagctctgca tcccataagc agagagatag ttagaatcag
     1681 gacatcctaa aaaccagggg tttctggcag gcattcacag cccgcccgaa accccaattg
     1741 cttaagatga tgacatttca aaaaattagc tcatcaagaa tttatcgtgg acgagtattt
     1801 gatctacacc aggacgaggt gcgtctgccc aatggacgcg ttgtccagct cgatatcgtc
     1861 caacaccccg gggcggtggt cttagtgccg gtggacgcgc agggcaatat ttggttcgta
     1921 cgccaatacc ggcatgcggc tggcacagaa atgctggaat tgcctgccgg gacgatggag
     1981 aggggtgaag atatcgaaag ctgcgcctat cgcgagctgc gtgaagagat cggtatggct
     2041 gccggagaag tattgaaaat cggtgagttt tttatggctc cgggttattc cacagagtat
     2101 gtatatattt acctagccac cagcttgcaa ccagcacccc tgccaggtga tgatgatgaa
     2161 ttcatccatg tcgagcgcat ggctgccgga gaagcgtttg agctggcaga aaagggacaa
     2221 atccccgatg caaaaacagt ggccgcgctc tccctggcgc gaccacatat ccaacggttc
     2281 taagaactgg caaattaagg tctagggcga tcgactcgcg ggggcatgct gcgcggtcga
     2341 ttttgtgagc gccctgcctc tgggttgagc agcgtattaa gccacttaat ggcgcgcggg
     2401 tcatattccc tacgcccgca cacatcacaa acccaggcgg ggaaatttgg gacggtgatc
     2461 aattcggtgt tcaaccaggt gaaataggtc aaagatcgca gacgcaaaac gcctgtttgg
     2521 cactctggac acagagccac gaatttattc gtttcgttcg tgtcttcccc agccatgatg
     2581 tgcttcctcc tgctattttc gagtttacca tgattttaca tgcaaatgga accttttttt
     2641 ggttaagata tcactgacaa tatt
//
LOCUS       DBNHOH010000813         2233 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1718336, whole genome shotgun sequence.
ACCESSION   DBNHOH010000813 DBNHOH010000000
VERSION     DBNHOH010000813.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1718336"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="AC16V7_14655"
     CDS             complement(<1..307)
                     /locus_tag="AC16V7_14655"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6258411.1"
                     /translation="MPSFRVLIVDNQRSVRRSLRSGLESTGQDFEVLEATSGEEAMLA
                     FASLPVDLLVTGVRLAGMSGLELLQRMRQNRPELKGILTAVNPDTRTGQLALKANANA
                     "
     gene            complement(322..1335)
                     /locus_tag="AC16V7_14660"
     CDS             complement(322..1335)
                     /locus_tag="AC16V7_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6258412.1"
                     /translation="MNEELLANGSAAEKNGKWDLVQVKRLVKYFPVRGGLFQRVVAWV
                     QAVDDVSFSIRQGETVGLVGESGCGKTTVGRTMLRLIEPTSGEVHFDGEEIFKLRGNE
                     LKAMRRNMQIIFQDPYASLDPRTPIGESIAEGLKIHKIGNARDRYDIVIEMLHKVGME
                     DYHARRYPHEFSGGQRQRIGIARALALRPKFIIADEPVSALDVSIQSQVLNILKDLQS
                     EFGLTYLFIAHNLSVVEHISDRVAVMYLGKMVEMADRDELFRNPLHPYTQALMSAIPI
                     PDPTLKRERIILQGDVPSPLNPPSGCRFHPRCPVAFEHCKVQEPPFKEVSTDHWVACW
                     RIE"
     gene            complement(1335..>2233)
                     /locus_tag="AC16V7_14665"
     CDS             complement(1335..>2233)
                     /locus_tag="AC16V7_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6258413.1"
                     /translation="KSVTSLSIMRLVGSPGKVVDGKILFEDRELLKMSEAEMIHMRGN
                     RMSMIFQQPQSSLNPVFKVGEQVAEVLRIHQKMDKSQAWQKAVDLLQLVGIPDAEKKA
                     HAYPHEMSGGQAQRVMIAMALALNPQLIIADEPTTALDVTIQAQILDLMRGLRSSMNT
                     AVILITHDLGIIAEMADRVAVMYAGYIVEQADVRKLFSNPLHPYTQGLIDSIPVLGQV
                     KDRLDVIPGNVPNLVDLPPGCRFASRCRSREEFELEICSQVEPDLLPAQNDHLVRCWL
                     YHDRKESGYKAPLKATLETEKQ"
BASE COUNT          523 a          628 c          564 g          518 t
ORIGIN      
        1 aagcgttggc gtttgcctta agcgcaagct gaccggtgcg agtatccggg ttcaccgctg
       61 tcaagatgcc cttgagttcc gggcgatttt gccgcattct ttgcaacaat tccaggccag
      121 acatacccgc caagcgcaca ccggtgacta ataaatccac cggcaagcta gcgaaggcta
      181 gcatggcttc ttcccccgat gtagcctcca gtacctcgaa atcttgcccg gttgactcca
      241 aaccagagcg cagtgaacgg cgtacgctgc gctggttgtc aacgatcaag acgcggaaag
      301 atggcatgat aaagggtttt tttactcgat gcgccagcaa gccacccaat gatcggtgct
      361 gacctctttg aaggggggct cctgcacctt gcagtgctca aaagcaaccg ggcagcgcgg
      421 gtggaagcgg catccagaag gcggattgag cgggcttggc acatcgcctt gaaggatgat
      481 acgctcgcgc tttagagtag gatcggggat cgggatagcc gacatcaagg cctgggtata
      541 aggatgcagc gggttgcgga ataactcatc acgatcggcc atttcgacca ttttacccag
      601 atacatcact gctactcggt ccgagatgtg ctcgacgacg ctcagattgt gggcgataaa
      661 caagtaggtc aagccaaact ctgactgcag gtccttaagg atattgagca cctgagactg
      721 gatagagaca tccagggcag ataccggctc gtcggcaata ataaactttg gacgcaacgc
      781 cagggcgcga gcaataccaa tgcgctggcg ttgacctccg gagaattcgt gcggatagcg
      841 gcgtgcatgg tagtcttcca tccccacttt gtgaagcatc tcgatgacaa tatcgtagcg
      901 gtcacgcgca ttcccaatct tatggatctt caagccttcg gcaatggatt cgccaatcgg
      961 ggtacgcggg tctagcgatg cataagggtc ctggaagata atctgcatgt tgcggcgcat
     1021 agcttttaac tcattcccgc gcaacttgaa aatttcctca ccgtcaaaat gcacctctcc
     1081 agatgtcggc tcgatcaggc gcaacatagt gcgcccaacg gtggtcttgc cacaccccga
     1141 ttcacccacc agccctacag tctcaccttg gcgtatggaa aagctgacat catctacagc
     1201 ctgtacccag gctaccaccc gctggaataa gcctccgcgt accgggaagt atttcaccag
     1261 ccttttaacc tgaactagat cccacttacc gttcttttct gctgcgctgc cgttggctaa
     1321 caattcttca ttcatcactg tttctctgtc tccaatgtgg ctttcaaggg agccttatag
     1381 cctgattctt tacgatcatg atacaaccaa caacgcacca aatggtcatt ttgagcgggt
     1441 aacaggtcgg gttccacctg ggaacaaatc tccagctcaa actcttcccg agagcggcag
     1501 cgcgaagcaa atcggcaacc tggcggaaga tcaaccaggt tcggcacatt ccctggtatc
     1561 acatccaggc ggtctttcac ctgacccagc actggtatcg agtcaatcaa gccttgtgtg
     1621 tagggatgca acgggttact aaagagtttc ctgacatcgg cctgttccac aatataacca
     1681 gcatacatca ccgccacacg gtcagccatc tcggcaatga tccccagatc atgggtaatt
     1741 aatatgactg ccgtgttcat gctgctgcgc agaccgcgca tgagatccag gatctgtgcc
     1801 tggatggtca cgtccagcgc agtggtgggt tcgtcggcaa tgataagctg cgggttcaga
     1861 gccagagcca tagcaatcat gacgcgctgc gcctgcccgc ccgacatctc atgtggatag
     1921 gcatgtgcct ttttctcagc gtcggggatg cccactagtt gtagaaggtc gactgccttc
     1981 tgccatgcct ggcttttgtc cattttttga tgaatgcgca acacttctgc cacctgctct
     2041 cccaccttga agaccggatt caggctggac tgcggttgct gaaaaatcat cgacatgcgg
     2101 ttaccgcgca tgtgaatcat ctcggcttcg gacattttga ggagctctct atcctcgaaa
     2161 agaattttgc catccaccac tttacccgga gagccaacca acctcatgat cgataaggat
     2221 gtgacgctct ttc
//
LOCUS       DBNHOH010000814         3070 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_51465, whole genome shotgun sequence.
ACCESSION   DBNHOH010000814 DBNHOH010000000
VERSION     DBNHOH010000814.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_51465"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="AC16V7_14670"
     CDS             complement(<1..632)
                     /locus_tag="AC16V7_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HIR6258414.1"
                     /translation="MGHTLAEKILAAKCGRPEVYPGQFINARVDLVLASELSGVLTIE
                     EFEKINGARIFDPKKVVLVMDHFTPAKDIKSAEIVKRCRDFAREHNITFYDVGRAGIQ
                     HVLLPEKGLVVPGDLIAGADSHTCTYGFMGAFGTGIGSTDAAAAMALGEMWLKVPESI
                     KLVYHGKPPKWVGGKDLILYTIKQIGVDGARYQAMEFTGEALDYLDMADR"
     gene            complement(716..1702)
                     /gene="hpaD"
                     /locus_tag="AC16V7_14675"
     CDS             complement(716..1702)
                     /gene="hpaD"
                     /locus_tag="AC16V7_14675"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018031834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HIR6258415.1"
                     /translation="MKFNITRAGHIEFRCTDLDRAREFYVDILGFVESDRDSERLYLR
                     GLEGREHHGLVLRKAESPGVTHIAFRVASPDDLDRLAELYQEQGRPIRWMPAGYEKGQ
                     GKALRVQDPSGLPLEFYHEMAGDRWLLQHFELYRGANVMRLDHFNCQVSEVQNAYDWF
                     TKKLGFYTSEVTVTEDSPPCLWAAWLHRKQNVHDLALMNGIGPRLHHAGFWLADQASV
                     LRACDVLAATGYADCIERGPGRHGISNAFFVYLRDPDGNRIELYTNDYMIPDPDWEPI
                     QWSINDPRRATFWGHKPPASWFDEASLVESIESGELMPTYPPPLKDRPEFVT"
     gene            complement(1724..2293)
                     /locus_tag="AC16V7_14680"
     CDS             complement(1724..2293)
                     /locus_tag="AC16V7_14680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR6258416.1"
                     /translation="MTEPIDTQQFRRTIGLFATGVTVIATETPEDIHAMTANAVTSLS
                     LDPLLVLVCIGKRARMAEILVREARFSINILRQEQQALSTFFAGSWRETKAPPFRFVH
                     WNGRPRLEGCLAALGCECHQTVDGGDHWIVIARVVELFQGIEPQLPLLFYRGRYGRLS
                     GEEGVPAPDLGWLEEPVQVFYDPWKDDSN"
     gene            complement(2290..>3070)
                     /locus_tag="AC16V7_14685"
     CDS             complement(2290..>3070)
                     /locus_tag="AC16V7_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase C-terminal
                     domain-containing protein"
                     /protein_id="HIR6258417.1"
                     /translation="TPGLKFLCRESVDYGRSAYDHPLGSRFEEMDAVVIFDDVFVPWE
                     RVFLYRDVDICNQAYAATGAIVHMSHQVLVKNIAKTEFMLGIASLMSNTIGIEVFQHV
                     QEKLAEIWINLETMKALLLAAEVNAAPDSYGVMRPAWDPLDAARNLYPRLYPRMVEII
                     QQLGASGLVAAPTEADVDGPLADEIKRFYQAARADAHERIPIFRLAWDVALSAFGSRQ
                     VLYERFFFGDPVRMAGALLRSHDFTPYMDTVRDFLAQREET"
BASE COUNT          681 a          873 c          832 g          684 t
ORIGIN      
        1 aagcgatcag ccatgtccag gtaatctagc gcctcgccag tgaactccat agcctgatag
       61 cgcgccccat ccacgccgat ttgcttgatg gtatagagga tcaaatcttt ccctcctacc
      121 cacttaggag gctttccgtg atataccagt tttatgcttt cgggtacctt cagccacatt
      181 tcacccaatg ccatggccgc ggcagcgtca gttgacccaa tgccagtccc aaaggcaccc
      241 ataaaaccat aagtgcaggt gtgggagtcc gctcctgcaa tcaggtcacc gggtaccacc
      301 aatccttttt ctgggagcaa tacgtgctgg atcccggcgc gccccacatc atagaaagtg
      361 atgttatgtt ctcgggcaaa atcccggcag cgtttgacaa tttctgcgct cttgatgtcc
      421 ttagctggag tgaagtgatc catcaccaat accacctttt tcggatcaaa gatgcgggct
      481 ccgttgattt tctcgaactc ctcgattgtc aggactccag atagctcaga tgccagcaca
      541 aggtcaacgc gagcgttgat gaattgtccc ggatagactt cgggtcgacc gcatttggcg
      601 gcgaggatct tttcagcgag cgtgtggccc atatatgctt ctccttgata gtacccaatg
      661 taacgacaaa gtgttgctga ctgcttgctc ccaggcctgc ggcagggcaa ataactcaag
      721 tgacaaattc agggcggtcc ttaagcgggg gcggataggt cggcataagc tccccgcttt
      781 caatcgattc aaccagggaa gcttcgtcga accaactcgc tgggggttta tgaccccaaa
      841 acgtcgcccg ccgcggatcg ttgatgctcc attggatcgg ctcccagtct ggatctggga
      901 tcatataatc atttgtgtag agttcgatgc ggttgccatc tgggtcgcgc aggtagacga
      961 agaaggcgtt gctaatacca tgccgccctg gcccgcgctc gatgcagtcg gcgtaaccgg
     1021 tggctgctag gacatcacag gcgcgcagca cgctggcctg gtctgccagc cagaaacctg
     1081 cgtggtgcag gcgcggccca atgccgttca tcagggcaag atcgtgcaca ttttgcttgc
     1141 ggtgcaacca ggcagcccat aagcatgggg gagaatcttc tgtcactgtg acctcggaag
     1201 tgtagaaccc caattttttg gtaaaccagt cgtaggcatt ttgtacctcg gaaacctggc
     1261 agttgaagtg gtctagacgc atcacgtttg caccacggta taactcgaaa tgttgtaata
     1321 accagcggtc tcccgccatt tcatggtaga actccagggg tagcccgctg ggatcctgga
     1381 cgcgcagcgc cttgccttga cctttttcgt aacctgcagg catccaccgg atagggcgtc
     1441 cctgttcttg atacagctca gccaatcgat cgaggtcatc gggactggcg acgcgaaagg
     1501 cgatatgcgt aacgccgggc gactcagcct tgcgtaacac aagaccgtga tgctcgcggc
     1561 cttccaggcc tcgcagatac aaccgctcgc tgtcgcggtc tgactccacg aaccccaata
     1621 tgtcgacgta gaattctcgc gccctatcga ggtctgtgca gcggaactcg atgtgccctg
     1681 ctcgagtgat gttgaacttc atacctgcta ccctccaaac caatcaattt gaatcatctt
     1741 tccatggatc gtaaaatacc tgtacaggtt cttccagcca gcctaaatcg ggcgcgggca
     1801 ccccttcttc cccagatagg cgtccgtaac gccctctgta aaacaataag ggtaactggg
     1861 gttcaatccc ctggaaaagc tctaccacgc gagcgatgac aatccagtga tcgcctccat
     1921 caaccgtttg atgacactca cagccaaggg ctgccaggca gccctccagt cgcggccgcc
     1981 cattccagtg cacaaagcga aatggcgggg ctttcgtctc acgccagcta ccagcaaaga
     2041 aggttgagag cgcctgctgc tcctgtcgca gaatgttgat ggaaaacctg gcctctcgta
     2101 ccagtatttc cgccatgcgg gcgcgctttc caatgcaaac cagcaccagc aggggatcta
     2161 aggatagaga tgtgacagca ttggctgtca tggcatggat atcctcgggc gtctctgtgg
     2221 caatgacagt cactccagtg gcgaacaacc caatcgtgcg gcggaactgt tgggtgtcga
     2281 ttggctcggt catgtctctt ctctttgggc taggaagtcg cgcacggtat ccatgtaggg
     2341 tgtgaaatcg tgcgagcgca atagggcgcc ggccatgcgc accgggtcgc caaagaaaaa
     2401 tctctcatag agcacctgcc gggagccgaa ggctgataag gccacatccc aggcaaggcg
     2461 gaagatgggg atgcgctcgt gggcatcagc gcgtgccgcc tggtagaaac gcttgatctc
     2521 atctgccaat ggaccgtcaa catcggcctc ggttggcgcg gccaccaatc cgctggcgcc
     2581 caattgctgg atgatctcga ccatgcgcgg gtagaggcgc gggtagaggt tgcgtgcagc
     2641 gtccagaggg tcccaggcgg gtcgcatgac tccatagctg tcaggcgcag cgttcacctc
     2701 tgctgccagg agaagagcct tcattgtttc gagattgatc caaatttcgg ctagcttttc
     2761 ctggacgtgt tgaaagactt cgatgccgat cgtgttggac atcagcgacg ctatccctaa
     2821 cataaattcg gttttggcga tgttcttcac cagaacctga tggctcatgt ggacgatggc
     2881 gccggtagcg gcgtaggcct ggttacaaat gtctacgtcg cggtacaaga acacgcgctc
     2941 ccacggcacg aacacatcgt cgaaaataac cacggcatcc atctcttcga accgtgagcc
     3001 gagggggtga tcatatgccg agcgtccata atctacgctt tctcgacaca ggaatttcaa
     3061 accgggcgtg
//
LOCUS       DBNHOH010000815         1408 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_51469, whole genome shotgun sequence.
ACCESSION   DBNHOH010000815 DBNHOH010000000
VERSION     DBNHOH010000815.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_51469"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="AC16V7_14690"
     CDS             complement(<1..1017)
                     /locus_tag="AC16V7_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015209715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258418.1"
                     /translation="MDPTPTNDLDRIIASAHRLGVEMDEEEALQWLTAIAASQAGDED
                     VVVDIHRGVFGHRVSMLDFDPKDLEHFREIGRLVEFQDVPDMVETALALSGSAAQSKI
                     QSFPGDCDYFERVNIKAATHAEACQILANLMREKALSTERGPTYQLIEVKFGSYPYDV
                     VVDGHPIKAGSPVSWTPEKIRDGQIGAFLPDGKPTAVRWEDVQTQPGWCKLDWVVADP
                     VRQQLANASNMLDVTWEAPDGTITPLDGYLDPYFQEVYLEAESIPIFSKLARHVSTDA
                     LDQYVAQLEKEVNKYLTKDVNYGKAAKRMYNVFRLTGRYEEAAFLRELFDEPTTLLYQ
                     IWSLI"
BASE COUNT          324 a          386 c          328 g          370 t
ORIGIN      
        1 gatcaatgac cagatctggt agagcaaggt tgtcggctcg tcgaataact ccctcaggaa
       61 agccgcctct tcgtaacgcc cggtcagccg gaagacgtta tacatgcgct tggcagcctt
      121 gccatagttt acgtctttgg tcaggtattt attgacctct ttttccagtt gggccacata
      181 ttggtctagg gcatctgtcg agacgtgcct tgccagcttg gagaatatgg ggatagactc
      241 agcttccagg taaacttctt gaaaatacgg atccaggtat ccatctaatg gggtgatggt
      301 gccgtctggc gcttcccagg tgacgtccag catattgcta gcatttgcca gctgctggcg
      361 gactggatca gctaccaccc aatccagctt gcaccagccc ggttgcgttt gcacatcttc
      421 ccagcgtacc gcggtgggct tgccatcggg gaggaaagcg ccaatctgcc catctcggat
      481 tttttccggc gtccaggaaa ccggcgatcc agccttaata ggatgtccat ccacaaccac
      541 atcatatgga tacgatccga acttgacctc gatgagctgg taggtcggcc cgcgctcagt
      601 gctcaatgct ttttcgcgca tcaggttagc caggatttga caggcttcag catgagtcgc
      661 ggctttgatg ttgacacgct cgaagtaatc gcaatcacca gggaagcttt gaattttgga
      721 ttgcgccgcc gagcccgaga gcgccagtgc ggtctcgacc atatcgggca catcttggaa
      781 ttcgaccaat cggccaatct cacgaaaatg ctccaggtct ttaggatcaa agtccaacat
      841 gctcacccga tggccgaata caccacggtg tatatccacc accacatctt catcgccagc
      901 ctgggaggcg gcaatagcgg tcagccactg taaggcctct tcctcgtcca tctctacacc
      961 caggcggtgt gcagaagcga taattcggtc aagatcgttt gttggcgttg gatccatcct
     1021 atttctccta aatgcgtgtt aaatatgtgg catacgcggt tgatcaaaaa gacttccaga
     1081 cctcgtatct attgtctgat tcactcaaag aacattgtgc cctaaccacc gttgccatac
     1141 gatcgaaacg gtagctgtgc accggagcta actactgtaa taataaacgt tccttttttc
     1201 tcttcgttac tcttcaaggg tagggctaat gaacttctca aatatcgagg ctattgggtt
     1261 atttaaccga atattcctac accccctttg tcgatcgaat tgccagccat ctgaccgctc
     1321 ttgatttaat caccccgacc gccacaaata ccttattgga cggcctcttg tctccaatta
     1381 ttgataactg atcaacgttt ttcttcca
//
LOCUS       DBNHOH010000816         2246 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_436070, whole genome shotgun sequence.
ACCESSION   DBNHOH010000816 DBNHOH010000000
VERSION     DBNHOH010000816.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_436070"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="AC16V7_14695"
     CDS             <1..743
                     /locus_tag="AC16V7_14695"
                     /inference="COORDINATES: protein motif:HMM:NF012888.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015016 - heparan sulfate
                     N-sulfotransferase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HIR6258419.1"
                     /translation="AMPKSGSHLIIQVLQGLTSLGPFVNPGFPPVNRGEDNRKLSDRA
                     VLANIQRMLPGDIGYGYISATPTFIDALTRPRRATVFVYRDPRDLIVSHIFYATQMHP
                     DHWMRRYYTETLSTMEERINAAIQGVDEPGSELTPIRRRYEGYLAWLEQPEVLSLRFE
                     DLVLNRQAVLKQLLDFIRGRGLNLKMPRVQAVSILDASVAPKKSGTFRKGQPGNWREH
                     FTDANKALFKEKTGDLLTRLGYETSDEW"
     gene            733..1587
                     /locus_tag="AC16V7_14700"
     CDS             733..1587
                     /locus_tag="AC16V7_14700"
                     /inference="COORDINATES: protein motif:HMM:NF012888.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015016 - heparan sulfate
                     N-sulfotransferase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HIR6258420.1"
                     /translation="MNGKTRLRLARRFLRRMAQSARWRRTLRGVPILFANSFPKSGTH
                     LLTQVLQGFPRLGPAVDSGLPAIVTFEGDTGRTRQPQEILADLQRLLPGDIAYGHVHA
                     LPQVVDFICREGVCAYFILRDPRDVAVSHVHYVTEMEPGHAHHRYYTEELHSFDERLQ
                     VSIQGIPGADVQFPDIRARFEPYLGWLERPEVLTLRFEDFISQRMETLGRVFDHAVQR
                     GFPASAERQDAIQTLAQVIDPHSSPTFRSGKAGTWRENFNSENKRFFKDVAGDLLVRL
                     GYEQDHDW"
     gene            1621..>2246
                     /locus_tag="AC16V7_14705"
     CDS             1621..>2246
                     /locus_tag="AC16V7_14705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012593431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR6258421.1"
                     /translation="MISIVIRAYNEERHIGRLLEGIAQQNLGETEIILVDSGSTDATL
                     EIARRYALRVVHIQPDEFTFGRSLNRGIAASQSEIIVIASAHVYPVYPDWLERLLAPF
                     SDPQVALAYGKQRGNATAKFSEGQIFARWYPESSDLQQAHPFCNNANAAIRRSLWQLH
                     LYDESLSGLEDLDWAHWAVGEGYRLAYVAEAEVIHVHNETPRAVYNRYR"
BASE COUNT          470 a          714 c          645 g          417 t
ORIGIN      
        1 acgccatgcc aaagtccggc tcgcacctga tcatccaggt gctgcagggg ctgacaagct
       61 taggcccatt cgtcaatcca ggcttcccgc ccgtcaaccg cggcgaggat aaccgcaagc
      121 tgagtgaccg tgcagtgctt gcgaacattc agcgcatgct acctggcgat atcggctatg
      181 gctatatcag cgccacgcca acctttatcg acgcgctcac cagacccagg cgcgctacag
      241 tctttgtcta ccgcgacccg cgcgatttga tcgtctcgca catcttctac gccacacaga
      301 tgcacccaga ccactggatg cgccgctatt acacggaaac gctgtccacg atggaggagc
      361 gcatcaatgc tgccatccag ggcgtggacg aacccggctc cgagctaacc cccatccgcc
      421 ggcgctacga gggctacctg gcttggcttg agcaacccga ggtgctcagc ctgcgctttg
      481 aagacctggt cttgaatcgc caggctgtat tgaaacaact gctggatttc ataagaggcc
      541 gcggtcttaa tttgaaaatg ccgcgcgttc aggcggtgag cattttggac gcctccgtcg
      601 ccccaaagaa gtccggtact ttccgcaaag ggcagccggg caactggcgc gagcacttca
      661 cggatgccaa caaagcccta ttcaaggaga aaaccgggga tttgctcacc cgtcttgggt
      721 acgagacgag cgatgaatgg taagacgaga ttaaggctgg cccgccgctt cttgaggcgc
      781 atggcacaga gcgcccgctg gcgccgcacc ttgcgaggtg tgcccatctt gtttgccaac
      841 tccttcccaa aaagcggcac gcacctgctg acccaggtct tgcagggctt tccaaggctt
      901 ggtccagccg tggatagcgg tctgcccgcc atcgtgacct tcgaggggga tacagggcgt
      961 acccgccagc cccaggagat cctcgccgac ctgcaacgcc tgctccccgg tgacatcgcc
     1021 tacgggcacg tgcacgccct gccccaggtt gtggacttca tctgccggga gggggtgtgt
     1081 gcatacttca tcctgcgcga tccgcgcgat gtggctgtct cgcacgtgca ttacgtcacc
     1141 gagatggagc cagggcacgc ccaccaccgc tactacacag aagagctgca tagtttcgac
     1201 gagcggctac aggtcagcat acagggcatc cccggggcag acgtacaatt cccagatatc
     1261 cgcgctcgct tcgagcctta tctgggctgg ctggagcgcc ccgaggtgct aaccttgcgc
     1321 tttgaagatt tcatcagtca gcgtatggaa actctggggc gcgtcttcga ccacgctgtg
     1381 cagcgcggct tccctgcatc cgcagagcgc caggacgcca tccagacctt ggcacaggtc
     1441 atcgacccgc acagctcacc cactttccgc agcggtaagg ctggcacctg gcgggagaat
     1501 ttcaattcag agaacaagcg ttttttcaaa gatgtagccg gagatttgct ggtacgcctg
     1561 ggctacgagc aggaccacga ctggtagagt aaatcagcaa tcagcgaaca gcacccagaa
     1621 atgatatcta ttgttatccg agcatataac gaggagcgac acatcgggcg actgctggaa
     1681 ggcatcgcgc agcagaacct gggcgagacc gagatcatcc tggtcgattc gggctccaca
     1741 gatgccacgc tggagatcgc ccggcgctat gcgctccgcg tggtgcatat ccagccagac
     1801 gagttcacct tcgggcgctc gctcaaccgg ggcatcgccg cctcgcagag cgaaataatc
     1861 gtcatcgcca gcgcacacgt ctatccggtc tatcccgact ggctggagcg actgctggct
     1921 cctttcagcg acccccaggt ggcgctggca tacggcaagc agcgcggtaa cgccaccgcc
     1981 aagttctcgg aaggacagat cttcgcccgc tggtacccgg aatcgtccga cctgcagcag
     2041 gcgcacccct tttgcaacaa cgccaacgcc gccatccgcc gttcgctctg gcagcttcac
     2101 ctctatgatg agagcctgag cggactagag gacctggatt gggcacactg ggcagtgggc
     2161 gaggggtaca ggctggccta tgtagccgaa gcggaggtga tccacgtgca caacgagacg
     2221 ccacgcgcgg tgtacaatcg ataccg
//
LOCUS       DBNHOH010000817         3717 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1526326, whole genome shotgun sequence.
ACCESSION   DBNHOH010000817 DBNHOH010000000
VERSION     DBNHOH010000817.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1526326"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="AC16V7_14710"
     CDS             <1..676
                     /locus_tag="AC16V7_14710"
                     /inference="COORDINATES: protein motif:HMM:NF020250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HIR6258422.1"
                     /translation="GDIKILEVPVELAPQGVITDLAQVTGRITKVRLVAGELVMEHNL
                     ADPTNVNHDLGFIIGDNQVLMAFPAQDLMSTLNVLQRGDLIDILVTLSQEVPTASEGV
                     GGEVVVREGDTVSRVLTFDAFQRLEITAMVVDIVQQEQRSASPVPAGEGTPQPQPTPQ
                     PSDVRLRAYLLALNPQDALLLKHLKDTGATFDFVLRAPTSNQLFELQTVTSDYLIDRY
                     ELSILK"
     gene            691..1947
                     /locus_tag="AC16V7_14715"
     CDS             691..1947
                     /locus_tag="AC16V7_14715"
                     /inference="COORDINATES: protein motif:HMM:NF012369.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpaE/TadZ family protein"
                     /protein_id="HIR6258423.1"
                     /translation="MATKAETSRVIFIGDPGAVQQQITAALGSQTDFQLVDVLSSTER
                     LIREIHVAEPNIILIDHIIGGKPTLDLIDDISLQYPDAAVITILPDNDPAVIQQVTLA
                     GARAFLVQPFTQVNLLSTLRRVTELEARRVKTQVVKESAKGEETKPMRSIAVFSPRGG
                     VGASTVALNLAIAMHEDTGARVLLMEGKFFFGHLAVMLNIRARNTIADLLPHASSLDE
                     GLVRDVVTEHASGIGVLLAPTDLQVGQGVRPDDVYSMLVNLERFFDYIVIDVGSYLTE
                     NTVTLMDTADKIMLVTTPDLASLHDASNFIQLSRSLGYPPDKIMIILNRANMEGGVRS
                     KDIEAALHHHVFAQIPDDHANALRSLNRGIPMIMHYSRSPVVKAIKQLSKRLPQNSGG
                     EPVRAKASPATSKSQSEALLASSHLG"
     gene            1966..2940
                     /locus_tag="AC16V7_14720"
     CDS             1966..2940
                     /locus_tag="AC16V7_14720"
                     /inference="COORDINATES: protein motif:HMM:NF012694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR6258424.1"
                     /translation="MLSPEIINLLFIAFAVGLAGIITILGMANVLAGSDDISERIETY
                     ALVPEEVERRSSGWGRSSLVRLRLRINTLLSFFASEEMGVRLLSANWPITETEFILIR
                     IGSVIVGFTIGWLLVRSPISGIGFAIIAFLIPEFLLRRSIYNRRRKFESQLVDVLVLM
                     KGAVRAGVGFLQSLDVVIQDMKAPASEEFRRVKREVGLGLPLSQALGNLHARMENDDL
                     YLLITALNINTQVGGNLSTMLEAVTLTIRERIQLFAEVRALTSMQRYSGYLLTLLPFA
                     TAALVFIMNPDYMGKLFEPGPFICIPIGALILVLLGNIVIRLITRIDI"
     gene            2970..>3717
                     /locus_tag="AC16V7_14725"
     CDS             2970..>3717
                     /locus_tag="AC16V7_14725"
                     /inference="COORDINATES: protein motif:HMM:NF012694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR6258425.1"
                     /translation="MIDIYILGILGLFLIVGGFFALVFSMRWFGSEELSSRLTNYVTV
                     VQAQSRQLTSTTAIRNRELTGSFTSRTLLPFFRQAGSFLGRLTPANSIEQLRRQLYIA
                     GYPLGLGPREFFGLRIAFIFFGFFLSILVLRRGINQTNLLVGAAVFIICFIFPILWLR
                     MLVNSRQDKLRKGLPDALDMLSVCADAGLGFDQSLQRISEHWSTPIGLELGRVVAEME
                     MGSSRREALRNLADRLDISEISSFVSVILQS"
BASE COUNT          837 a         1006 c          956 g          918 t
ORIGIN      
        1 cggagatatt aaaatcttgg aggttcccgt agagctggcg ccccaaggcg taattaccga
       61 cctggcgcag gtcactgggc gcatcaccaa ggtgcgttta gtggctggcg aattggtcat
      121 ggagcacaac ctggccgacc caaccaacgt gaaccacgac ctgggcttta tcatcggcga
      181 taaccaggtc ttgatggcct tcccggctca agatctgatg agcacactca atgtgctcca
      241 gcgtggcgac ctgatcgaca tcctggtcac cctcagccag gaagtaccca cggcaagcga
      301 aggcgtggga ggggaagttg ttgtgaggga gggcgatacg gtatccaggg tcttgacttt
      361 cgacgctttt cagcgcctgg agatcacagc catggttgtg gacatcgtac agcaagagca
      421 gcgcagcgca tccccggttc ctgctggcga aggcacacct cagccccagc caaccccgca
      481 gccctcggat gtgcggctac gggcatacct gctggccctc aacccgcaag atgccctgct
      541 gctcaaacac ctcaaggaca ccggcgccac cttcgacttc gtcttacgcg cgccgacctc
      601 aaaccagctc ttcgagctac aaactgttac ttcagactat ctcattgacc ggtatgaact
      661 gtcgatatta aagtagtaaa ggaaaaactt atggcaacca aggcagaaac ctctcgcgta
      721 attttcatcg gcgatcccgg cgctgtccag cagcagatca cagccgcgct gggcagtcag
      781 acagattttc aattggtgga tgtgctttca tccaccgagc ggctgatccg ggaaattcac
      841 gtcgccgagc cgaatatcat cttgattgat catataatcg gcgggaaacc cacgctggac
      901 ttgattgatg atatctcgct ccaatatccc gacgcggccg tcatcactat cttgccggat
      961 aacgaccccg cagtcatcca gcaggtcacc ctggctggcg ctagggcatt tctggtccag
     1021 ccgttcaccc aggtgaatct cctcagtaca ctgcgccgag taaccgagtt agaggccagg
     1081 cgcgtcaaga cccaagtggt gaaggagagc gctaaaggtg aagagacaaa acccatgcgt
     1141 agcatagctg tcttcagccc gcgtggcggt gttggcgcct caactgttgc tttgaacttg
     1201 gcgattgcaa tgcacgaaga taccggcgca cgcgtgttgc tcatggaagg gaaatttttc
     1261 ttcggtcacc tggctgtgat gctcaacatt cgcgcccgca acaccatcgc tgacctgctg
     1321 ccacacgcta gctccctgga tgagggtttg gtgcgcgatg tagtcacaga acacgcctcc
     1381 ggaattggtg tcttgctggc acccactgac cttcaagttg ggcagggtgt ccgtccggat
     1441 gacgtttata gtatgctggt aaatttggag cgcttcttcg attacatcgt catcgatgtg
     1501 ggcagctatc tcaccgaaaa cacggtgacc ctgatggata cggccgataa gattatgctg
     1561 gtcaccaccc cagacctggc ttccctgcat gatgccagca actttatcca attaagccgt
     1621 tcgctaggct acccacccga taaaataatg atcattctaa atcgtgccaa catggagggt
     1681 ggagtgcgct caaaagatat cgaagctgcc ttgcaccacc atgtgttcgc gcaaatcccc
     1741 gacgatcatg ctaacgccct acgtagccta aaccgcggta tccctatgat catgcactac
     1801 tcgcgcagtc ctgtagttaa ggcgatcaag caactctcca agcgactccc ccaaaatagc
     1861 ggaggagagc ctgtccgggc caaagccagc ccggccacca gcaaaagcca gagcgaagcg
     1921 ctgctcgcct catcgcattt gggataatga taaagaacta gtcttatgtt gtcaccagag
     1981 attatcaacc tgctcttcat agcgttcgcc gttgggctcg ccggcattat cacaattttg
     2041 gggatggcga atgtgctggc cgggagtgac gatatcagtg aacgtatcga gacctatgcc
     2101 ctcgttcccg aggaagtgga acgcagatcg agcggttggg ggcgctccag cttagtccgc
     2161 ttgcgattgc ggatcaacac cctactctct ttctttgcct ctgaggaaat gggagtgcgc
     2221 ttgctaagtg caaactggcc gatcactgaa acagagttta ttctcatccg catagggagc
     2281 gtcattgtag gttttactat aggttggttg ttagttcgct ccccaatatc agggatcggc
     2341 ttcgctatta tcgctttcct gataccagaa ttcttgctgc gccgaagcat ctacaatcgc
     2401 cggcggaagt tcgagtcaca acttgttgat gtactggtgt tgatgaaagg agcagtacgt
     2461 gccggtgtgg gatttttgca atccctcgat gtcgtcatcc aagatatgaa ggcgcctgcc
     2521 tccgaagaat ttcgccgggt aaaacgggaa gttggtttag gattgcccct ctcccaggcg
     2581 ctcggcaatt tgcatgcgcg catggaaaac gacgatttat acctgctgat caccgctctt
     2641 aatatcaata cccaggtggg tggcaatttg tcaacaatgc ttgaagcagt gaccctcacc
     2701 atccgagagc gcattcaatt atttgctgag gtccgggcat tgacctctat gcaacgatat
     2761 tccggctact tgctcacgct gctgcccttt gccacagccg cgctggtttt cataatgaat
     2821 ccagactaca tgggcaagct atttgagccc ggcccattta tatgcatccc tataggtgcg
     2881 ctgatcctgg ttctacttgg gaatattgtg atccgcttaa ttacccgcat cgatatataa
     2941 gctgcatttt ctagaattga taattaccaa tgattgatat ttacatacta ggaatacttg
     3001 gactgttctt gattgtgggt ggattcttcg ccctggtatt tagcatgcga tggtttgggt
     3061 cggaagaatt gagttcccgc ctcaccaatt acgtcaccgt agtacaggct caatcccggc
     3121 agctcacctc caccacagcc atccgcaatc gtgagcttac tgggtctttc accagccgca
     3181 ccttgctgcc attttttcgt caggctggct cctttcttgg gcgattaact cctgcaaatt
     3241 cgatcgagca actcaggcgt cagctatata tcgctggata ccccctgggg cttggcccgc
     3301 gtgaattttt tggcttgcgt attgcattta tattctttgg attttttctg tcaattttgg
     3361 tgctgcggag aggaatcaac cagacaaatc tactagtcgg tgcagccgtc ttcattatat
     3421 gttttatctt cccaatcttg tggctgcgca tgttagtgaa ctcacgtcag gacaaattga
     3481 gaaaaggcct tcccgacgcg ctggatatgc tcagtgtctg tgcagatgct ggtctgggat
     3541 ttgaccagtc gttgcagcga ataagtgagc actggtccac accgattggg ctcgaattgg
     3601 ggcgcgtggt ggctgaaatg gagatgggct cgtcacggcg ggaggctctg cgcaatctcg
     3661 ccgaccggct ggatatctct gaaatttcta gctttgtatc cgttatcctg caatccg
//
LOCUS       DBNHOH010000818         2847 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1205564, whole genome shotgun sequence.
ACCESSION   DBNHOH010000818 DBNHOH010000000
VERSION     DBNHOH010000818.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1205564"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1687
                     /locus_tag="AC16V7_14730"
     CDS             <1..1687
                     /locus_tag="AC16V7_14730"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR6258426.1"
                     /translation="VHASDYDQTSLGIGLIYPGYLTEKPGFFYSKNRFDPLEILFAQS
                     INDNKKRWYPFYPQEQLNALLDITRLLSQAYGLERILVSQEINQFDLISGPAFPINRL
                     RQLVEGEGTATELMEETSSEADLFLQPGGVGPKLLNQPVPAHTPIVVIDDDEGWVLVE
                     VMAALGDRRWTVGWMKANQVTAKPFVPKVSHDHLLFTQQDRQIKFIAAHEKNYNPKKE
                     LDPRYVVIHFTTGTHMQQTIHTFLNPGNGVSTHLLVGRSGQVVQFVPFDRVAFHCGLS
                     TWEGERDLNHYAIGIEVDNAGNLRKSPKGFKSRDKFIPAEQVKFKRHWKERGERPWQT
                     FTPEQIRVVQDIVKALKERYPTIQEILGHDMVNLINRLDPGPFYPLGELREAILGDAQ
                     QVIKSYQVTENCPIYENLGNQPPRLLPHPDFGELPQNSTVRVKEVHDHWSLVKVKQSS
                     KANLKEKLGWVLSNSIEPEEGKAKTKFPQTLYKLIPAAQVRLPPLEIKASPLPAGDHV
                     RIQFNVGERWALVAPVLEVRKDAEGNYEMVIPKDKVKKRYLEGWVEKKFLKEI"
     gene            complement(1758..2594)
                     /locus_tag="AC16V7_14735"
     CDS             complement(1758..2594)
                     /locus_tag="AC16V7_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258427.1"
                     /translation="MPTAVPPCASAVLPVQRHAIIYASLTLLAALFAGLACNFVTNAR
                     AEPPTATPTVLVEPTSTPLHAPTPLPTATASPSATPSPTHSPTATPTITPIPTYTILR
                     GQVLVRSNCRYGPGAPYLYKYGLVVGSNLEVIGRNDLGTWILVRAIGGDNPCWVKATL
                     MDVKGDVMNVAPTYIPLPQSPYYDPPKWVIAKREGDEVTIFWKRVQYRAGDETAEAPY
                     LVEAWLCTAGELVFTPIGSYETQIKVIDEAGCGEPSYGRLYFVEKHGYSRWLKIPWPA
                     HE"
     gene            2566..2847
                     /locus_tag="AC16V7_14740"
                     /pseudo
     CDS             2566..2847
                     /locus_tag="AC16V7_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
BASE COUNT          634 a          820 c          868 g          525 t
ORIGIN      
        1 agtccacgct agcgactacg atcagacctc gctagggatt gggctgatct atcccggtta
       61 ccttactgaa aaaccgggat ttttctactc aaaaaatcgg tttgaccctc tcgagattct
      121 ttttgcgcaa tccatcaacg acaataaaaa acgctggtat ccgttctacc cccaggaaca
      181 gctaaatgcc ttgctagaca tcacccgcct gttgagccag gcttatgggc ttgagcgcat
      241 actggtcagt caagaaatca accaattcga tctcatctcg ggtccggctt ttcccatcaa
      301 ccgccttcgg cagctggtgg agggtgaggg cacagccaca gaattgatgg aagagacgtc
      361 ctcagaagcg gatttattcc tgcagccggg tggggtaggt cccaaactgc tcaatcagcc
      421 agtccccgcc cacaccccaa tcgtggtcat cgacgacgac gagggttggg tgctggtgga
      481 ggtgatggct gccctagggg accggcgctg gaccgtcggc tggatgaaag ccaaccaggt
      541 gacggccaag ccgttcgtac cgaaagtaag ccatgaccac ctgctgttta cacagcaaga
      601 ccggcagatc aaatttatcg ccgcgcacga aaaaaactac aaccccaaga aagagttgga
      661 cccgcgctat gtcgtcatcc acttcaccac cggcacccac atgcagcaga cgatccacac
      721 cttccttaac ccggggaatg gcgtctccac ccatctgctg gtcgggcgca gcgggcaggt
      781 ggtgcagttc gtgcccttcg acagggtcgc ttttcactgc gggctttcca cctgggaagg
      841 agaaagggac ctgaaccact acgccatcgg gatcgaagtg gataacgccg ggaatctacg
      901 taaatcgccg aagggattca agagcagaga taagttcatc ccggcggaac aggtcaagtt
      961 caagcggcac tggaaggaaa ggggcgaacg gccctggcag acctttacac cagagcagat
     1021 ccgggtggta caggacatcg tgaaggcgct caaagagagg tatcccacca tccaggaaat
     1081 cctggggcat gatatggtca atttgatcaa ccgcctggat cccggcccgt tctatcccct
     1141 cggcgagctg cgcgaagcca tactgggcga tgcacagcag gtgatcaagt cttatcaagt
     1201 cacggagaac tgcccaattt acgaaaacct gggcaaccag ccacccaggt tgctgcctca
     1261 ccccgatttc ggcgagctgc cccagaacag cacggtgcgg gtcaaagagg ttcacgacca
     1321 ctggtcactg gtgaaagtaa aacagagctc gaaggccaat ttaaaggaaa agctgggctg
     1381 ggtgctctcg aactccatcg agcccgaaga gggcaaagcc aaaacgaagt tcccccaaac
     1441 actctataag ctcatccctg ccgcccaagt gcgcctgcct cccctggaga tcaaggccag
     1501 ccctctgccg gcgggagacc atgtgcgcat ccagtttaac gtaggcgagc gctgggcgct
     1561 ggtggcgcca gtgctggagg tgcgcaagga tgctgaaggc aattacgaga tggtcatccc
     1621 gaaagacaag gtaaagaagc gctacctgga aggctgggtg gagaaaaaat tcctcaagga
     1681 aatttaaatt ccccgcccca gccccctata tttcccttcc actcggcaga aggggggaag
     1741 atagcggggt gattgcctca ctcgtgggcc ggccagggga ttttgagcca gcgcgaatag
     1801 ccgtgctttt ccacgaagta caaccgcccg taggatggct ctccacagcc ggcttcatcg
     1861 atgaccttga tctgcgtctc gtaggagccg atgggcgtga agaccagctc tccagccgtg
     1921 cacagccagg cttcgaccag atacggggct tcggcggtct catcccccgc ccggtactgc
     1981 acgcgcttcc agaagatggt cacctcgtcc ccctcccgct tggcgatgac ccacttgggc
     2041 ggatcgtaat atggcgactg gggcagcggg atgtaggtgg gggcgacgtt catcacgtcc
     2101 cccttgacat ccatcagcgt tgccttgacc cagcaggggt tgtcgccgcc gatggcgcgc
     2161 accaggatcc aggtgcccag gtcgttgcgc ccgatcacct ccaggttgct ccccaccacc
     2221 agcccgtatt tatacaggta gggcgcgccg ggaccgtagc ggcagttgga gcgcaccagc
     2281 acttgcccgc gcaggatggt gtaggtgggg atgggggtga tggtaggcgt ggctgtcggc
     2341 gagtgggtgg gcgacggtgt cgcgctgggt gaggccgtcg ccgtggggag aggcgtgggc
     2401 gcgtgcaggg gtgtggaggt tggctcgact aagacggttg gcgttgcggt ggggggctcc
     2461 gcccgggcat ttgtgacaaa attacaggcc agcccggcga acaaggccgc cagcagggtc
     2521 aggctggcat atataatggc gtgtctttga acaggtaaga cggcgctggc gcagggcggt
     2581 accgcggtcg gcaccgggat caatgcgcat cccaaattcg gcgccaaggt ggcgacgctg
     2641 ctggcagaga gaacggcgat tgcattcacg caggcggact ccctgttcga aggcctgagc
     2701 agccaggaca cggccgttga aatctccggg caacttcggg tgctcgccat gagcctgatg
     2761 aagattgcga acgacctgcg ctggatgaac tcaggcccgc tcgcgggcat aggcgagatc
     2821 gaattgccgg cattgcagcc cggttcg
//
LOCUS       DBNHOH010000819         2320 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1718766, whole genome shotgun sequence.
ACCESSION   DBNHOH010000819 DBNHOH010000000
VERSION     DBNHOH010000819.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1718766"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="AC16V7_14745"
     CDS             <1..730
                     /locus_tag="AC16V7_14745"
                     /inference="COORDINATES: protein motif:HMM:NF013279.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR6258428.1"
                     /translation="FIQPDLSAVITILFLGGTLFFLAGGDLRQIGVMLIIAILVGWLV
                     VVVNTTGGERVSSYLAGLKDPTNASYHVQRSLEAFVNGGWLGVGIGNARTKLTGLPVP
                     PTDSIFAVVGEETGVFGATVLVILFVIMLWRGLSIARRAPDQLGTLLAAGMSLWLAME
                     ALVNMAVMLNLLPFAGNALPFISAGGSNLVVSFAAIGILMNISRTSVKKKEEGRSFVA
                     VVDLRRRNRRGRVSRTRRTASNDE"
     gene            651..1820
                     /locus_tag="AC16V7_14750"
     CDS             651..1820
                     /locus_tag="AC16V7_14750"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - obsolete lipid glycosylation
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HIR6258429.1"
                     /translation="MRLLICAGGTGGGVYPALAVLQAMMNDERQTRDDDLRPGSIVHD
                     LLWIGGLGGMEANLVKQTGVPFKAIPAAGIHGVGWRALPGNAWQLGRGLLAARQLLRE
                     YKPDVLFFTGGYVAVPVALAARLPGRGYPQPRSLLYIPDIEPGLALKSVARFADQIAV
                     TTEDSREYFGSHADVVVTGYPVRNSLRMWDKDQAMEALGLKPDLPVLLVFGGSKGARS
                     INQALWSALTELLNEMQIVHITGERDWHEVEAILERLKASQAEDFYVRYHPYPYLHHE
                     MGAAFTAADLAICRAGASTLGELPLFGLPAVLVPYPHAWRYQEINAQYLVRRGAAVLV
                     NDASLVEQLLPVVRQLMGNFEKRAQMRLAMSALAQPNAAKQIVRLLDDLSAGLRR"
     gene            1824..>2320
                     /locus_tag="AC16V7_14755"
     CDS             1824..>2320
                     /locus_tag="AC16V7_14755"
                     /inference="COORDINATES: protein motif:HMM:NF014704.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HIR6258430.1"
                     /translation="MMTLTFAFWLFAFLFAIIGAMRGWAKELLVTFSVILSLFFLEVL
                     GRYFPPVRDYLAPPNGSAEFWVRAIVVMMLSFFGYQTPNITKLAGTRFARERLADSLL
                     GFFLGLLNGFLIVGTLWYFMEDSNYPFPKYITKPTPPPTLLAYLAPRWLGIPTIYFAI
                     AVSFL"
BASE COUNT          449 a          579 c          675 g          617 t
ORIGIN      
        1 cttcatacag cccgacctga gtgctgtgat caccatatta tttttgggcg gtacgctgtt
       61 ctttctggcc ggtggtgacc tgcggcagat cggcgttatg cttatcatcg ccatactggt
      121 aggctggctg gtcgtcgtgg tgaacacgac gggaggcgag cgtgtatcca gctatctagc
      181 cgggttaaag gatcccacaa atgcgtctta tcacgtccag cgttcattgg aggcttttgt
      241 caatggcggt tggttaggcg ttggcatcgg caatgcgcgc accaagctca ccggcctgcc
      301 tgtgcctccc actgacagca ttttcgccgt ggtaggtgag gagaccggcg tatttggggc
      361 aacggtattg gtgatcttgt tcgtcatcat gctgtggcgc gggttgagca tcgctcgtag
      421 agctccggac cagttgggta ccttactggc agctgggatg agtttatggc tcgcgatgga
      481 agccttagtc aatatggcag tcatgctgaa cttactgccc ttcgccggga atgccctgcc
      541 atttatcagc gctggtggat ccaatcttgt ggtatctttt gctgctattg gaattttgat
      601 gaacatttct cggacttctg tcaagaaaaa ggaagaaggg aggtccttcg ttgcggttgt
      661 tgatttgcgc aggcggaacc ggcgggggcg tgtatcccgc actcgccgta ctgcaagcaa
      721 tgatgaatga tgagagacaa acgagggatg atgaccttcg tcccggatcc atcgttcacg
      781 atttgctttg gattggtggt ttaggtggca tggaggctaa cctggtgaag caaactggtg
      841 tgccctttaa agcgatcccg gctgcgggca ttcatggcgt aggctggcgc gcactgcctg
      901 gtaatgcctg gcaacttggg cgcggcttac ttgcggcgcg gcagttactt cgcgaatata
      961 aaccggatgt gcttttcttt accggtggct acgtggctgt gccggttgct ttggcggcgc
     1021 gattgcccgg acgcggctac ccccagccgc gtagcttgct gtacattcct gatattgagc
     1081 ccggcttggc attgaaaagc gtggctcgct ttgcagacca gatagccgtc accacggaag
     1141 atagccgcga atatttcggc agccatgcag atgttgtagt cacagggtat cccgtccgta
     1201 acagcctgcg catgtgggat aaagatcagg ctatggaagc cttgggtctc aagcccgatt
     1261 tgccagtctt gctcgtcttt ggaggcagta agggtgcacg gtcgattaat caagcattgt
     1321 ggtctgccct cacggagctg ttgaacgaga tgcaaatcgt gcatatcacc ggagagaggg
     1381 attggcatga agtggaagct atccttgaac ggttgaaagc ctcgcaagcg gaagactttt
     1441 atgtccgata ccacccctac ccatacttgc atcatgaaat gggggcagcc tttactgccg
     1501 ctgatctggc gatctgccgg gcaggggcat ctacgctagg agaactgccg ctgtttggcc
     1561 tgccagccgt attagtcccg tatccgcatg cctggcgtta ccaggagatc aatgcccaat
     1621 atcttgtcag gcgcggggcg gcggttttag tcaatgatgc ttccctggta gaacagttat
     1681 tgccggtggt gcgccagctg atgggaaatt tcgagaaacg cgctcagatg cgcttggcga
     1741 tgagcgccct agcccagccc aatgcggcca aacagattgt aaggctctta gacgatctgt
     1801 ccgcaggtct gcggaggtga tcaatgatga cattaacgtt tgccttctgg ttgtttgcct
     1861 ttttattcgc tatcattggg gcaatgcgag gttgggcgaa ggaactattg gtgaccttca
     1921 gcgttatcct gtccctgttt ttcttggagg tacttgggcg ttattttcca ccagtgcgag
     1981 actatctggc tccaccgaat ggttcagcag agttttgggt gcgcgcgatc gtggtgatga
     2041 tgctgtcctt ctttggatac cagacaccca atataactaa attggctggg acacgcttcg
     2101 cccgcgagcg cctggcagat tcactcctgg gctttttcct ggggttattg aatggctttt
     2161 tgattgtcgg taccttgtgg tattttatgg aagactcaaa ctacccgttc cccaagtata
     2221 ttaccaagcc gactccgcct cctacgctgc tggcttacct cgccccacgc tggttgggca
     2281 tcccaaccat ctatttcgca attgcggtct cattcctgtt
//
LOCUS       DBNHOH010000820         2362 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_244066, whole genome shotgun sequence.
ACCESSION   DBNHOH010000820 DBNHOH010000000
VERSION     DBNHOH010000820.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_244066"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="AC16V7_14760"
     CDS             <1..849
                     /locus_tag="AC16V7_14760"
                     /inference="COORDINATES: protein motif:HMM:NF015764.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6258431.1"
                     /translation="LTSFVDSATMFMLGDKKEQYGRVRLGGTFGFGIAAPLAGVLVQT
                     YGLKMAFWGCAILLLMALIVSQKLAHNPQKASDTTGQGVRTLVTNPRWLLFLAVAFAG
                     GLGIAGTNTYLFPYLKELGASESIMGLALTLGTIFEIPVLFFGYRLLHRFKPYGLYML
                     AMVLTGLRLLAFAASGTPNLVLFIQLLNGLTFPAMWMAGVAYADEHAPAGLSTAAQGM
                     FGAMVFGFGTAVGGFIGGPLLDNLGGSGLYLVFGGIVLVIVALAYLVQWLLPAEPKKS
                     PTVAMN"
     gene            1034..1984
                     /locus_tag="AC16V7_14765"
     CDS             1034..1984
                     /locus_tag="AC16V7_14765"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6258432.1"
                     /translation="MQNHSRTALPERSVLIAFFLFILVGGGASVAIRITYAELAPFWA
                     AASRFTLAALILWGIVFYKRIPIPKGRALVGALLFGTLTVGLAFLLIGWGLVATPASR
                     YQILMATVPLLTVFLSSLHGIEAISGRGIFGSLLAVGGIAFTAGGSSASQVSMPHIAA
                     ILLAAVFIAEGGVMIKKFPPNPPVMTNAIGMTIGGLILGVASLVSGEKWTIPTQTTTW
                     IAFIYLVIFVTIVSFLLYMFVLRKWSASGTSYGFVLVPLVTIVVASTLAGEEITTNFL
                     LGAGLVLAGALVGALLPSKAKPEIVNECKDRSGQVLPRCV"
     gene            complement(2009..>2362)
                     /locus_tag="AC16V7_14770"
     CDS             complement(2009..>2362)
                     /locus_tag="AC16V7_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR6258433.1"
                     /translation="KQAMAAIRAYEYELSRALLDVLEETPGVTIYGIKDMRRLEERVP
                     TFAFNLQGKHPRLLAEALDKECIYVWDGNYYALAVTERLGLEESGGMVRVGPVHYNTV
                     EEVYRFGEALRKIVA"
BASE COUNT          470 a          651 c          632 g          609 t
ORIGIN      
        1 cttacatcct tcgtggacag tgccaccatg ttcatgttgg gagacaagaa agaacagtat
       61 ggccgtgtcc gcctgggtgg gacgttcggc ttcgggatag cagccccact tgctggggtt
      121 ctcgtccaaa cgtacggctt gaagatggcg ttctggggct gtgcgatcct gttgctgatg
      181 gcgcttatcg tcagtcagaa attagcccac aatccgcaga aagcaagtga tacgaccggg
      241 caaggcgtgc gcacgctggt gacaaacccc cgttggctac ttttcctggc ggtggccttt
      301 gcaggcggtc tgggaattgc cggcacgaac acctatctct tcccttactt aaaggagttg
      361 ggagcgagtg aatcgataat ggggctcgcg ctcacgcttg gaacaatctt tgagatccca
      421 gtgctctttt ttggatatcg acttctccat cgcttcaagc cctatggact gtacatgctg
      481 gcgatggttc tcaccgggct gcgtctactg gcgtttgctg cctctggtac gccaaatctc
      541 gtcctattta tccaactgct taacggcctg acttttccgg ccatgtggat ggcaggcgtg
      601 gcctatgccg atgaacacgc tcctgccggt cttagtactg ctgcacaagg gatgttcggc
      661 gcgatggtct ttggatttgg cacagcggtc ggtggtttta tcggcggtcc gctattggat
      721 aacttggggg ggagcggtct ttaccttgtc tttggaggca ttgtgctggt catagtggca
      781 cttgcttacc tggtgcaatg gcttttgccc gcggagccga agaaatcacc gactgtcgcg
      841 atgaactgag ccaggcgggg agaaataatc atagcggcct gacgcgcacc cagcagccgc
      901 gatgaaactc aaactcgttg aattgtgaga aatatttttt ggtctctact aggccatgct
      961 ataatatcta ataaattaga tgatataact ccattatacg agatgattca ataaaggatg
     1021 tgtcatgata ggaatgcaaa accattcacg cactgctcta cccgaaagat ctgtactgat
     1081 cgcctttttt ctttttattc ttgtcggtgg tggcgcgtct gtggcgattc gcattaccta
     1141 cgcggaactt gcacctttct gggcggctgc atctcgtttt accctggcag cccttatatt
     1201 atggggaatc gttttctaca agcgaatccc catacccaaa gggcgtgcct tggtgggcgc
     1261 gctgctattc ggaactctca ctgttgggtt ggctttcctg ctgatcggct ggggactcgt
     1321 ggctaccccg gccagccgct accagatact gatggcgact gtgccgcttc tgaccgtatt
     1381 cctttcctcc ctgcatggca tcgaagccat tagcgggcgc gggatttttg gctcgctgct
     1441 agccgtgggt ggcattgcct ttaccgcggg tgggtcttca gcctcgcaag tgtcgatgcc
     1501 tcacatagct gcgattctcc tggctgctgt tttcatcgcc gaaggcgggg tcatgatcaa
     1561 gaaattcccg ccaaacccgc cagtcatgac aaacgccatt ggcatgacca tcggtggctt
     1621 gatcctaggg gtcgcctcgc tggtcagtgg cgagaaatgg accataccga cccaaacaac
     1681 cacttggatc gctttcatct atctcgttat cttcgtaacg attgtgtcat tcctgctata
     1741 tatgttcgta ttgcgcaagt ggtcggcctc agggacatcc tacggattcg tgctggttcc
     1801 gctcgtcacc atcgtcgtag cctcaacctt ggccggagag gaaatcacga ccaatttcct
     1861 gcttggcgca gggctcgtgc tagctggggc acttgttggc gccctgctgc cttcaaaggc
     1921 aaagccggaa attgtaaacg aatgcaagga ccgttctgga caggtattgc cgcgctgcgt
     1981 ttgaggcgca ataagcgaaa gactctgcct aggccactat cttcctcagc gcctccccaa
     2041 acctgtacac ctcctcaacc gtgttgtagt gcaccggccc aacgcgcacc ataccgccgc
     2101 tttcctctaa ccccaatcgc tctgtaactg ccagggcata atagttgcca tcccacacgt
     2161 agatacactc tttatctagc gcttctgcca acaggcgcgg gtgcttgccc tgcagattga
     2221 aagcaaaggt cggcacgcgc tcctccaggc ggcgcatatc cttgatcccg tagattgtca
     2281 cgccaggtgt ctcttccagc acatccagca gggcgcggga gagctcatac tcataagcgc
     2341 ggatagctgc catggcttgc tt
//
LOCUS       DBNHOH010000821         2408 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_244221, whole genome shotgun sequence.
ACCESSION   DBNHOH010000821 DBNHOH010000000
VERSION     DBNHOH010000821.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_244221"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="AC16V7_14775"
     CDS             complement(<1..690)
                     /locus_tag="AC16V7_14775"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011975921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - L-methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HIR6258434.1"
                     /translation="MDQRLPSTTEDGTSWVGRVRSGSRLERVLRSGRFAVTAELNPPD
                     SADPQSVYDRALVLAEVCDAINATDASGANVHMSSVGVCALLTRAGYEPVMQVSCRDR
                     NRIAIQGDLLGAAAMGVRNVLCLTGDGVQVGDQPEAKPVFDLESITLLRTARMLRDQG
                     HFLSGRQLDVKPKFFLGAAENPFIPPYDFRSLRLAKKIEAGAEFIQTQYCFDFRRLCK
                     FMDSVRDMGLHE"
     gene            complement(615..1091)
                     /locus_tag="AC16V7_14780"
     CDS             complement(615..1091)
                     /locus_tag="AC16V7_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HIR6258435.1"
                     /translation="MFRRLQDSPHFLEVVYRSVYRMLLPFRPWLKPDGWVERVFVQLE
                     RLSKGALFDCRMCGQCVLHYTGMTCPMTCPKNLRNGPCGGVRLDGNCEIYPEKACVWV
                     QAWQRSQQMPAYGGEILHIQAPLNYSLKDTSAWINDFHLQQKMAPVGWDESGQVPA"
     gene            complement(1092..1394)
                     /locus_tag="AC16V7_14785"
     CDS             complement(1092..1394)
                     /locus_tag="AC16V7_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011975919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HIR6258436.1"
                     /translation="MTVTYQIVYWRDIPAQIRLRSPQGRISRPLSPRFQEAIDAAAMR
                     AHATSTDDYLEDWRTGDTQSREGDAQQVADSVADEIEAAYPQDRLLGLAANGGLEK"
     gene            complement(1391..1972)
                     /locus_tag="AC16V7_14790"
     CDS             complement(1391..1972)
                     /locus_tag="AC16V7_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HIR6258437.1"
                     /translation="MKTALIACGALAREVLALKEKHGWDVEVLALPSLLHNEPARIPG
                     AVQERIRQARESHDRVLVVYGDCGTGGLLDRLLAKEGVQRVAGPHCYEMYADGTFERL
                     MAEAPGTFFLTDYLVQSFDHLVLEGLGIDKHPELRDEYFRNYTRVVYLAQRDDPALLE
                     RARWAADCLALPLEVHKAGYGKLESRLKELIEA"
     gene            complement(1969..>2408)
                     /locus_tag="AC16V7_14795"
     CDS             complement(1969..>2408)
                     /locus_tag="AC16V7_14795"
                     /inference="COORDINATES: protein motif:HMM:NF014615.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR6258438.1"
                     /translation="LEEVRAAVEGVRRISVDIPVITTMTFDTHGRTMMGVKPEDAATT
                     LSSYGAVAVGGNCGNGVEEIITVVEKMHAVEPEAILVAKANAGIPKLVGGQAVYAATP
                     QDMADYAVKAFAAGARVIGGCCGSTPAHIQAISTALASQVVAG"
BASE COUNT          497 a          771 c          663 g          477 t
ORIGIN      
        1 ttcgtgcaat cccatatctc gtacagagtc cataaatttg cataagcggc gaaagtcgaa
       61 acaatactgc gtctggataa actccgcccc agcctcgatt ttcttggcca gccgcaggga
      121 gcggaaatcg tagggtggga taaatggatt ctcggctgcc cccaaaaaga acttgggttt
      181 cacatccagt tgcctgccgc ttaaaaagtg cccctgatcg cgcagcatgc gtgctgtgcg
      241 cagcagggtg atcgattcaa gatcaaagac gggcttggcc tctggctgat cgcccacttg
      301 cacgccatca ccggtcaggc agagcacatt gcgcacgccc attgccgctg cgcccagcaa
      361 gtctccctgg atagcgattc ggttgcggtc gcggcaggag acctgcatga ccggctcgta
      421 gccggcacgc gtcagcaaag cacacacccc aacgctggac atgtgcacgt tggcacccga
      481 ggcgtccgtg gcattgatgg cgtcgcacac ctctgccagc accagcgctc tatcatacac
      541 cgactgggga tcggcgctgt ccggggggtt gagttcagcg gtcactgcaa accgcccaga
      601 gcgcagcacg cgctctaagc gggaacctga cctgactcgt cccacccaac tggtgccatc
      661 ttctgttgta gatggaagtc gttgatccat gccgaggtat ctttcaaact gtagttcagc
      721 ggagcttgaa tgtgcaggat ctccccacca taggctggca tctgctggga gcgctgccag
      781 gcttgcaccc acacgcaagc cttttccggg taaatctcgc agttgccgtc cagccgcacc
      841 cctccgcaag gtccattgcg caggttcttg gggcaggtca tggggcaggt catgccggtg
      901 taatgcaaca cgcattgccc gcacatgcgg cagtcaaaaa gggcgccctt gctcaagcgc
      961 tccaactgga cgaacactcg ctcaacccac ccgtctggct ttagccaggg tcggaaaggc
     1021 agcagcatgc ggtacaccga ccggtaaaca acctcaagga aatggggtga atcttgcaaa
     1081 cgacgaaaca cttacttttc taggcccccg ttcgccgcca atcccaacaa ccgatcctgc
     1141 gggtaagcgg cttcaatctc atctgccact gagtcagcca cttgctgcgc atcgccctcg
     1201 cggctctgtg tatcaccggt acgccagtcc tcaaggtagt cgtcggtact ggtggcatgg
     1261 gcacgcatag cggctgcgtc gattgcctct tggaaacgcg gcgaaagtgg gcgagagatg
     1321 cgcccctgcg gagagcgcaa gcgtatttgg gccgggatat cccgccaata gacgatctgg
     1381 taggtgacgg tcatgcctcg attaattcct tcaacctgct ttccagcttg ccgtagcctg
     1441 ccttgtgtac ctccaagggg agagccagac aatcagctgc ccatctagcc ctttccagca
     1501 gcgcaggatc gtcacgctgg gcaagataca ccacgcgggt gtaattacgg aaatattcgt
     1561 cgcgcagctc ggggtgttta tcgataccca gcccctccag caccaggtgg tcaaaggact
     1621 gcaccaggta atccgtcaag aagaacgtgc cgggtgcctc agccatcaag cgctcaaacg
     1681 tgccgtcggc gtacatctca taacagtgcg gtccagccac gcgctgcacg ccttccttcg
     1741 ccagcagccg gtcgagcaag ccacccgtgc cgcagtcgcc atagactacc agcactcgat
     1801 catggctttc tcgcgcctgg cggatacgct cctggactgc cccaggaatg cgcgcaggct
     1861 cgttgtgcag caaggagggc aaagccagca cctccacatc ccagccgtgc ttctccttca
     1921 gcgccaacac ctcacgcgcc aacgcgccac aggcgattag ggcggttttc atcctgctac
     1981 cacttgagat gccagggctg tggagatggc ctggatatgg gcaggtgtgc tgccacagca
     2041 gcctccaatg acgcgggcgc ccgctgcgaa ggctttaacg gcgtaatcag ccatgtcctg
     2101 cggggtggct gcatacaccg cttgcccgcc caccagcttg gggatgcccg cgtttgcctt
     2161 ggcaaccaga atcgcttctg gctcaactgc gtgcatcttc tccacaacgg tgatgatctc
     2221 ctccaccccg ttgccgcagt tgcctcctac cgctacggca ccataggatg acaaagtcgt
     2281 ggcagcatct tccggtttca cgcccatcat ggtgcgcccg tgggtatcaa aggtcatggt
     2341 ggtgatcacg gggatatcca ccgagatacg ccgcacaccc tccaccgcag cccgcacttc
     2401 ttctaggt
//
LOCUS       DBNHOH010000822         1188 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_51859, whole genome shotgun sequence.
ACCESSION   DBNHOH010000822 DBNHOH010000000
VERSION     DBNHOH010000822.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_51859"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="AC16V7_14800"
     CDS             complement(<1..1032)
                     /locus_tag="AC16V7_14800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR6258439.1"
                     /translation="MTISSSSSQMDLTSAKKEAARVTLLGMWLDIALGLMKIVGGLLT
                     ASFALVADGIHSLSDAVSDIFVLVVSHYSHEAPDTEHQYGHGRFEAIGTVVMGMLFFA
                     IASVLIYDCYLRLAGHRVMQVPGPWGILLAAISIAAKEWIYRYTMRTANLLNSSLLQA
                     NAWHSRSDAISSIAVLMGLVGAQQGYPWLDLVAAIFVGLIIARIGWRLCLDSLKELVD
                     TAIPADREAQVRSQILGVEGVQEVTGLRSRQAGGKVILEAQVQVDPRITVSEGHQLGE
                     AVASSITGRFADINDVIVHVDPQNPDHLGPQLLPLREEVVARIIDSWKHLVLAAEIKN
                     IDLHYLDDGI"
BASE COUNT          268 a          350 c          332 g          238 t
ORIGIN      
        1 gattccgtca tccagatagt ggaggtctat gttcttgatc tccgccgcaa gaaccagatg
       61 cttccagcta tcgatgatac gtgcgacgac ttcctctctt aggggaagca gctgtggccc
      121 gaggtgatca gggttctggg ggtctacgtg aacgataacg tcgttgatgt cggcaaacct
      181 cccggttatg gaactggcga ccgcctcccc gagctggtgc ccctcggata cggtaatccg
      241 tggatccacc tggacctggg cctccaggat cacttttcca ccagcctgcc tgctgcgcag
      301 gcctgtcact tcttgcacgc cctcgacgcc caggatctgg cttctgacct gcgcctcgcg
      361 gtcggcgggg attgccgtgt cgaccagctc cttgagactg tccaggcaaa gccgccagcc
      421 gatgcgggcg attatcagac cgacgaagat cgcagccacc aggtcgagcc agggatagcc
      481 ctgttgcgcg cccacaagtc ccatcagaac cgcgatcgat gaaatggcat ccgaccggct
      541 atgccatgcg tttgcctgca ggagcgagga gttcagcagg ttcgctgtgc gcatggtata
      601 gcggtagatc cattccttgg ccgcaatcga tatcgccgcc agcagaatgc cccacgggcc
      661 tgggacctgc atgacccggt ggcccgccag ccgcaggtag cagtcgtaga taaggaccga
      721 ggctatcgcg aagaaaagca tgcccatgac aaccgtacca atggcctcga agcgaccatg
      781 gccgtactgg tgctcggtgt cgggggcctc atgggagtaa tggctgacca ccaggacgaa
      841 gatatccgag acagcatcgg agagggagtg tattccgtcg gcaaccagcg cgaatgatgc
      901 cgtgagcagc ccgccgacaa tcttcatgag accaagggct atatccagcc acatacccag
      961 cagggtgacc cgcgcagcct ctttcttggc actggtgaga tccatttgcg atgagctgga
     1021 gctaatggtc aagccgaatc ctaactgctt atttttacga caaaaaaaag cccgacaagt
     1081 gtcgggctca atatactatc aagggagaga taaatgaaac aaaacccaca attttgttca
     1141 accatctacc tacacttact ctgactgcgg gttgtgcaca aagttccc
//
LOCUS       DBNHOH010000823         2650 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_757804, whole genome shotgun sequence.
ACCESSION   DBNHOH010000823 DBNHOH010000000
VERSION     DBNHOH010000823.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_757804"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1475)
                     /locus_tag="AC16V7_14805"
     CDS             complement(<1..1475)
                     /locus_tag="AC16V7_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258440.1"
                     /translation="MNQFDELDTQIAQLMEREAATLAELRNLLLEVEEFINSEQYQSL
                     SIDERNRLQAARKEVIGRIQGEENGASDAATSESEPSTLDESLESATPSTLESPAQAE
                     GVKGHNPVAEGQMEVAEKLFYSGRYAESIQLFDRVIQLEPNWERAKQHRSEAENYLRT
                     GYIPAVALPADAASAYGKAQSAARVGRYSDAMALLEKAQNILREMGIQRWQEGQEFAQ
                     KLQESVDAENVYEEGLASFQLGQLDQAIEKVETAARATGIPKYTDKAGALRQVKENLR
                     SMTELLNQANIEPQAASQIKTNLDALIAEHGANPAFDRMIERLKSVIPRVVEPLKEQT
                     RALKNQAERATTLEEALYLARQAKQNLNQIRNLEGVDESLDRLQTEIDGLQRLIQKYD
                     FDVQSAMRSYENRPSWPAEAARLSAEVRERYPNDPAVSRLNRNLRGYRWKLMGIRIAG
                     ILLGIILLAFLGFLGMGRIRAYLISLTPTPTPTATATPTFTP"
     gene            complement(1475..>2650)
                     /locus_tag="AC16V7_14810"
     CDS             complement(1475..>2650)
                     /locus_tag="AC16V7_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258441.1"
                     /translation="DLILDALKGLRKGIKHADLIIEHPEVLNEIPWLSEYRSREILSL
                     PGSRPLRLEREEIAPELVKTVTGVEEGKLGANQDIHLAEPLDIWVPEARGSSARVFTG
                     HLRNRAGQTEQYALKVMRPKRIEYALPLFREEVQVLSILRDVPGISPLVECGFLRIEE
                     GLDFPSDETHRSAAHLEGQVVRYGAEEAQNFLASMERYIAQGWLPYLALVKRDPKHNL
                     MVYCDAGYTHGWFLPLREGLLLAIQICDILQIAHDRNIVYRDHKILHFYWEPQPPGVV
                     MIDWNIAKRQPEGLSEAEKQFDLVQFGARALHHIITGRPAPGALPVGPNRPEEIERSA
                     LSYTVSWTYDDERLPNRVKEILEQALNQGYTHVKALRQDLAQVQQQAPEPAQNRGKK"
BASE COUNT          504 a          747 c          702 g          697 t
ORIGIN      
        1 ggggtgaagg tcggcgtggc tgtcgccgta ggtgttggcg tgggggtcag ggagatcagg
       61 taagccctaa tcctacccat gcccaggaaa ccgaggaaag ctaagagaat gatcccgagc
      121 aagatgccgg ctatcctgat ccccatgagt ttccaccgat agcctcgtaa gttgcggttc
      181 aagcggctca ccgccggatc gttgggataa cgttctctta cctccgcgct caagcgggcg
      241 gcctcagcag gccagcttgg cctgttttca taagaccgca tggcagattg cacgtcaaaa
      301 tcgtatttct ggatcagcct ttgcagccca tcgatttcag tttgcagtcg atccaggctt
      361 tcatccaccc cctccagatt gcggatctgg ttcaggtttt gctttgcctg ccgcgccagg
      421 tacagggctt cttccaatgt tgttgcccgc tcagcctggt ttttcagggc acgagtttgt
      481 tctttcagag gttcaacaac tcgcgggata acggatttca accgctcgat cattcgatca
      541 aatgccgggt tcgctccatg ttctgcgatc agcgcatcca ggttggtctt gatctgcgag
      601 gccgcctgtg gctcgatatt ggcctgatta agcaactcag tcattgagcg aaggttttct
      661 ttgacctggc ggagagcccc agccttatcc gtgtatttcg ggatacccgt ggcgcgcgcg
      721 gcggtttcta ctttttcgat ggcctggtct agctgtccaa gttggaatga agctaacccc
      781 tcttcgtaga cattctctgc atcgacgctc tcctggagtt tttgggcgaa ctcctgacct
      841 tcttgccagc gctggatgcc catctcccgc aagatgttct gcgccttttc caacagcgcc
      901 atagcatccg aatatcgccc taccctggca gccgattggg cttttccgta ggcggatgca
      961 gcatcagcgg gcagagccac cgcggggata taaccggtgc gcaggtagtt ttcggcctct
     1021 gagcggtgct gtttggcccg ctcccaattc ggctctaatt ggatgacacg gtcgaagagc
     1081 tggattgact ctgcataccg cccgctataa aacagtttct ccgccacttc catctgccct
     1141 tccgcgaccg ggttatgccc ctttacccct tctgcttggg cagggctttc aagtgttgat
     1201 ggggtggctg actccaggct ctcgtccagg gtggaaggct cactttccga agtggctgca
     1261 tcgcttgccc cgttttcctc cccttgaatg cgaccaatca cttctttacg ggctgcctgg
     1321 aggcggttgc gctcatcgat ggaaagcgac tggtattgtt cggaattgat aaattcctcg
     1381 acttccagca gtaaatttcg caattcagcc agggtggctg cctcacgctc catcaattgg
     1441 gcgatttgtg tatccaattc atcaaactgg ttcattactt tttgcctctg ttctgggctg
     1501 gttcgggtgc ttgctgttgt acctgcgcca gatcttgcct caatgccttg acgtgagtat
     1561 acccctggtt aagtgcctgc tccaaaatct ctttgacccg gtttggcagg cgctcatcgt
     1621 cgtatgtcca gcttaccgta tagctcagcg cgctgcgctc aatttcttct gggcggtttg
     1681 gccccacggg cagtgcgcct ggagcaggtc gtccggtgat gatgtggtgg agcgccctag
     1741 cgccgaattg taccaggtca aattgctttt cggcttccga taaaccctcg ggttgccgtt
     1801 tggcaatgtt ccagtcgatc atcacaaccc ctgggggctg gggttcccaa taaaaatgca
     1861 gaattttgtg gtcgcggtaa acgatattcc ggtcatgggc gatttgcagg atgtcgcata
     1921 tttggattgc cagtaataga ccttctctga gaggcaagaa ccagccatga gtataccctg
     1981 catcgcaata caccatgaga ttatgcttgg ggtctctttt caccagcgct agatatggta
     2041 gccagccttg agcaatgtag cgctccatgg aagccaggaa attttgggct tcctctgcac
     2101 cataacgcac tacttgcccc tctagatgag cagcactgcg gtgggtctcg tcgctgggga
     2161 aatctagccc ctcttcgatg cgaagaaagc cgcattcgac cagcggggaa atgcctggca
     2221 catcccgcag tatcgaaagc acctgcactt cttctctaaa cagcggcagc gcatactcga
     2281 tccgctttgg tcgcatcacc ttgagggcgt actgctctgt ttgacctgcg cgattgcgca
     2341 aatgccctgt gaacaccctg gcagatgagc cgcgcgcttc tggcacccag atatctaacg
     2401 gctccgccag gtgaatgtcc tgattggcac cgagtttgcc ttcttctact cctgtaaccg
     2461 tctttaccag ctctggcgcg atctcttctc tttccagcct caagggtcga gagccaggta
     2521 aagatagaat ctcgcgtgag cggtattcgc tcaaccaagg aatttcattc aacacttcag
     2581 ggtgctcaat gatcaggtcg gcgtgcttga tcccttttcg caggcctttg agggcatcca
     2641 agatcaggtc
//
LOCUS       DBNHOH010000824         2391 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1526737, whole genome shotgun sequence.
ACCESSION   DBNHOH010000824 DBNHOH010000000
VERSION     DBNHOH010000824.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1526737"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="AC16V7_14815"
     CDS             <1..543
                     /locus_tag="AC16V7_14815"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR6258442.1"
                     /translation="RDVLLNPTRTGLLDALRAMGGEITVQNSSERGGEPVGDITVTHS
                     RLRCTQVDGPLVVRMIDEFPAFAVAATYARGRTDVREAAELRNKESDRIAVLSRELRA
                     IGANIEETRDGFTIQGDGALRGGLVNPSGDHRLAMALAVAGLAAQQTVTVQAAEIIGE
                     SYPDFVNGLQSLGAGMRLES"
     gene            540..1469
                     /gene="aroE"
                     /locus_tag="AC16V7_14820"
     CDS             540..1469
                     /gene="aroE"
                     /locus_tag="AC16V7_14820"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIR6258443.1"
                     /translation="MMREAYQLGLIGFPLEHSLSPHLHRAALRAGGLDGEYHLYPIPP
                     GPNRSNRIRELLSRMRIGEIHGLNVTVPLKQVVMPFLDTLTPTARSVGAVNTIYLNDE
                     GLVGDNTDVQGFWVDLMRQLTLDNDLPPLALVLGAGGAARAVVYALIQAGWQVIVAAR
                     RPEQARELVESLSHAVKGEISPSQVERSNQSRQLLHPTKLSTDTIREVLAAMNDDTNA
                     LALMVNATPLGMFPHTTVSPWPPEITFPRNAFVYDLVYNPAETLLMKQARQAGLPTAN
                     GLGMLIEQAALAFERWTGLVASREAMRWKILEK"
     gene            1483..>2391
                     /locus_tag="AC16V7_14825"
     CDS             1483..>2391
                     /locus_tag="AC16V7_14825"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR6258444.1"
                     /translation="MPIAQRMQALPAHFFASLIARISALQAKGLDVIRLDEGAPDLPP
                     PPPVIQALNSSAEDPTHHSYQPHRGTRNLREAWATMYRHAYDVGLDPETEILPLLGSK
                     EGIFHLPMAIINPGDTVLVPEPGYITYTRGTLFAGGEPVYFPLLPERGYLPDLEAIPA
                     ETAHKAKMMWLNYPNNPTTGVATLEFFADAVDFAKQYDLVLCHDAAYTQVTFDSHRAP
                     SLLEIPGAKEVVVEFNTLSKSHNMAGWRVGAALGNAQLLRNLYTLKTNLDSGHFLPVL
                     DAAIEAMCGDQSWLGDRNKIYRQRRDA"
BASE COUNT          546 a          613 c          685 g          547 t
ORIGIN      
        1 cgggatgtgc tcctgaaccc aacccgtaca ggcttgctgg acgccctgcg agcgatgggt
       61 ggagagatca cggtgcaaaa tagttctgag cgtggtggag agccagtggg ggatattacg
      121 gtcacgcaca gccgattgcg ctgtacccag gtagatggcc ctttggttgt gcgcatgatc
      181 gatgaatttc ctgcatttgc cgtcgcagca acctacgcgc gtgggcgtac cgatgtgcgc
      241 gaggcagccg aattacgtaa taaggagtct gacaggattg cagtgctcag cagggaactg
      301 cgagcaattg gtgcaaatat tgaagaaacc cgcgatggat tcacgatcca gggagacggg
      361 gcattgcgag gtggtctggt gaatccatcc ggcgatcatc gattggcaat ggcactggcg
      421 gtcgcagggc tggctgcgca acaaacggtc acagttcagg cagcagagat catcggcgaa
      481 tcctatccgg atttcgttaa cgggctgcaa tccctggggg caggtatgag gctggaatca
      541 tgatgcgtga ggcataccaa ctgggtttga tcggctttcc cttggagcac agtctctcac
      601 cgcacttgca tcgggctgcc ctgcgagcgg gtggattaga tggggagtat catctgtatc
      661 ctatcccgcc aggcccaaac cgctcgaata ggatacggga attactaagc cgtatgcgca
      721 taggcgaaat tcacggattg aatgtgactg ttccgttgaa gcaagtggtg atgccttttt
      781 tagatacgct caccccaaca gctcgatccg ttggtgcggt caatactatc tatctcaacg
      841 atgaagggtt ggtaggggat aataccgatg tgcagggatt ttgggtagat ttgatgcgcc
      901 agcttaccct agataatgac cttccgcctc tcgctttggt ccttggggca ggtggagccg
      961 cccgtgcggt ggtgtatgca ttgatccagg caggttggca ggtcattgtc gctgccaggc
     1021 gtccggagca ggcaagggag ctagtcgagt ccctttctca tgcagtcaaa ggagagattt
     1081 caccctccca ggttgaaaga tcgaatcaga gtcgccagct gctccacccc accaagctat
     1141 caacagatac catcagggag gtcctggcgg ctatgaatga tgacacgaat gccctggctt
     1201 tgatggtcaa tgccacaccc ttgggcatgt ttccgcatac gaccgtgtct ccctggccgc
     1261 cggaaatcac ctttccgcgc aatgcttttg tgtatgattt ggtctataat cctgctgaga
     1321 ccctgctgat gaaacaagct agacaggcag gattacccac cgccaatgga ttaggtatgc
     1381 tgatcgagca ggctgccctg gcgttcgaac gttggacggg actggttgcc tcgcgggaag
     1441 ccatgcggtg gaagattctg gaaaaataag gattgagaaa atatgcctat tgctcaacgt
     1501 atgcaggctt tgccggctca tttttttgca tccctgatcg cgcgaatcag cgcactgcaa
     1561 gccaagggat tggacgtcat ccgtttagac gaaggcgcac cagacctgcc tccgcctcca
     1621 ccagttattc aggccttaaa ttcgtccgct gaagacccaa cgcaccacag ttaccagccg
     1681 caccgcggca cgcgaaatct gcgtgaggct tgggcgacga tgtaccggca cgcctatgat
     1741 gttggtttag atcctgagac cgaaatactg ccacttttag gatccaagga aggcatcttt
     1801 cacctgccga tggcgataat caacccaggt gatacggtgc tggtcccaga accaggctat
     1861 atcacgtata cgcgtggaac gctcttcgca ggtggggagc cagtatattt tccattacta
     1921 cctgagaggg gctatttgcc tgatttagaa gccatccccg ctgaaaccgc ccataaggcg
     1981 aagatgatgt ggctgaatta tccgaacaat cccacgaccg gtgtagcgac cttggaattc
     2041 ttcgctgatg cggtagactt tgcgaagcaa tatgacctgg ttctatgcca tgatgccgcc
     2101 tacacacagg ttaccttcga cagccatcgc gctcccagtc tgttggagat tccaggggca
     2161 aaagaggtgg tggttgaatt taataccctt tcgaaatcgc acaatatggc cggctggcgt
     2221 gtaggtgctg ctctgggaaa tgcacagctg ctcagaaact tgtacacact caaaaccaac
     2281 ctggacagcg gtcatttttt gcctgtgctg gatgccgcca tcgaagcgat gtgtggcgat
     2341 cagagctggc tgggcgatcg caataaaatc taccgccagc ggcgggatgc g
//
LOCUS       DBNHOH010000825         2432 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1719084, whole genome shotgun sequence.
ACCESSION   DBNHOH010000825 DBNHOH010000000
VERSION     DBNHOH010000825.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1719084"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="AC16V7_14830"
     CDS             complement(<1..326)
                     /locus_tag="AC16V7_14830"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018302335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6258445.1"
                     /translation="MNDTAVPAVRLSNVSRLFGDFAAVDDVSLDIQDGEFFTMLGPSG
                     SGKTTCLRLVAGFDRPTYGTILLHGKDVTQLPPYEREVNTVFQDYALFPHMSVGDNVG
                     YGLMID"
     gene            complement(387..1586)
                     /locus_tag="AC16V7_14835"
     CDS             complement(387..1586)
                     /locus_tag="AC16V7_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017516924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6258446.1"
                     /translation="MKANKILFRWLVLLASLSLVLTACGGGAATPAPEAEGGPLQSIG
                     EGEGAVSIIAWAGYIERGETDPNFDWVTQFEKDTGCMVSVKVAATSDEMVALMNEGGF
                     DLVTASGDASNRLISSGRVQEINIDLIPSWSTVDERLQNAPWNTVDGKHYGTPYQWGP
                     NVLMYNTDVFPTPPDSWSVVFEEQTLPDGPSNKGRVQAYDGPIYIADAALYLMVNNPE
                     LGIKDPYNLDRDQFNASLDLLRQQRQIVGRYWHDAFVQMDDFTNEGFVASSSWPFQVN
                     LLQFEDKPIGSVFPKEGATGWSDTTMMHVDAPHPNCAYKWLEHSLNPKLQGDLAAWFG
                     SVPAVPAACTGNELLTDEGCDINGMKDFDKIHFWKTPTTDCGDGRQECVPYHEWVTNY
                     IAVIGGR"
     gene            complement(1698..2180)
                     /locus_tag="AC16V7_14840"
     CDS             complement(1698..2180)
                     /locus_tag="AC16V7_14840"
                     /inference="COORDINATES: protein motif:HMM:NF046293.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR6258447.1"
                     /translation="MAKYDHLDQDILKLLNLNARISSAQIARELEESARTVQNRIQRL
                     TELGAVQFVGVVSPASFGYSLAVDIFCELEVGYQEQALQAIQDMPNITYVAISTGDQD
                     ISLQAIFKNGADMNDFITNKLHQVPGMRRTRTVLLPRILKDNYQWMPPDDDFEHDHNG
                     "
BASE COUNT          610 a          646 c          595 g          581 t
ORIGIN      
        1 ttatcgatca tcagcccgta acctacgtta tctcccacac tcatgtgtgg aaataaggca
       61 taatcctgga aaaccgtgtt gacctcccgc tcataaggcg gcagttgggt gacatccttt
      121 ccatgcaaca agatagtccc ataggttgga cggtcaaaac cagcgaccaa acgcaggcag
      181 gtcgttttac cagagcccga tggccccagc atcgtaaaaa actcgccatc ctggatgtcc
      241 aggctgacat cgtccactgc ggcaaaatcg ccaaacaggc gactcacgtt cgataaacgg
      301 actgcgggaa cggctgtatc gttcatttat tccaaacctg gatgaacctc ccgcagtatc
      361 gatcctgcgg gaggttcaag aattacctat cgcccgccga tgacggcaat atagttagta
      421 acccattcat gatagggcac gcactcctgg cgcccatcac cacagtcggt agtgggtgtc
      481 ttccagaagt ggattttatc gaagtccttc atgccattga tgtcgcagcc ctcgtcggtg
      541 agcaattcat tgcctgtaca agccgcggga acagctggca ccgaaccaaa ccaggcagcc
      601 aggtcgcctt gcagcttggg attgagggaa tgctctaacc acttgtaggc gcagttagga
      661 tgcggggcat ccacatgcat catggtggta tcagaccagc cggtggcgcc ttctttgggg
      721 aagacgctgc cgattggctt gtcctcaaat tgcagcaggt taacctggaa aggccaagag
      781 ctcgaggcga caaaaccctc gttagtgaag tcatccatct ggacgaaagc gtcatgccag
      841 tagcgcccta cgatctggcg ctgttggcgg agcaggtcaa gagaagcatt gaactggtca
      901 cggtccaggt tatagggatc tttgataccc aattcaggat tattgaccat caaataaagc
      961 gccgcgtccg cgatgtagat gggaccatcg taagcctgga cgcgcccctt gttggatgga
     1021 ccgtcgggca acgtctgttc ttcgaagacc acactccagc tatcaggcgg agtggggaat
     1081 acatctgtat tgtacatgag gacattaggc ccccactggt atggcgtgcc ataatgcttc
     1141 ccatcgaccg tgttccaggg ggcattctgc aggcgttcat caaccgtgct ccagcttgga
     1201 ataaggtcga tgttgatttc ctgaacccta ccagaagaga tcaggcggtt actggcatcg
     1261 ccagaggcgg tgaccaggtc aaatccgcct tcgttcatca gggcgaccat ctcgtcggac
     1321 gtggcggcta ccttcacact caccatgcag ccagtgtcct tctcaaattg ggtaacccag
     1381 tcgaagttcg ggtcggtctc gccgcgctcg atatatccag cccaggcgat gatggagaca
     1441 gcgccctcac cttcaccaat cgattgaagc ggtccaccct cagcctcggg ggctggtgtc
     1501 gccgccccac ctccacaggc agttaatacc aggctgagac tcgctagcaa aaccaaccat
     1561 cgaaacagga tcttattagc tttcatcact ttctcctttg gaaaaatttt gttttcgaac
     1621 tatggcaggt caatgtatct cacatcatgt tcaacaaaca atcatcacct ccaatccgat
     1681 ggaattaagt gcgatgctca gccattgtgg tcatgttcga aatcatcatc cggcggcatc
     1741 cactggtagt tatctttgag aatacgcggg agtaagactg ttcgcgtgcg gcgcataccc
     1801 ggaacttggt gcagtttgtt ggtgataaaa tcgttcatat cagcgccatt cttaaaaatc
     1861 gcctgcaagc tgatatcctg atctccggtt gaaatcgcca catatgtgat gttcggcata
     1921 tcttggatgg cttgcagagc ttgttcttga taccccacct ctaactcgca aaagatgtcc
     1981 accgccaaac tgtaaccaaa cgaagctgga ctcacaacac ctacaaactg gacggcgccg
     2041 agctctgtca atcgctggat gcggttctgc acagtgcgag cagattcctc taattcacgt
     2101 gcgatttgag cgctggatat gcgtgcattc aggttgagca gcttgagaat atcctggtcc
     2161 aggtggtcgt atttcgccat aattggtctt ggtgagaata taagttagta tactttactt
     2221 ggcttaaatg tcaatagaaa aatagttttt tgtattattc ggaaaaatca aatggataat
     2281 atttccaaat cagcataagc tgagccatga gcactattga acaggcataa aattaacccc
     2341 tagcgtgcgc gcaaattccc cgtcacacac cagctaacag gatgggatat ccaagcatgt
     2401 caaagttcta accccctctt cctagttcag ag
//
LOCUS       DBNHOH010000826         3362 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_821007, whole genome shotgun sequence.
ACCESSION   DBNHOH010000826 DBNHOH010000000
VERSION     DBNHOH010000826.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_821007"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="AC16V7_14845"
     CDS             complement(<1..758)
                     /locus_tag="AC16V7_14845"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing response regulator"
                     /protein_id="HIR6258448.1"
                     /translation="MAKGRLLIVEDDTDISTMLRIFFNGLNYEVDIAARGSDALEKTR
                     QNLPHLIVLDIMLPDIDGFEVCRILRTNTRTSHVPIIFLTQKDERSDKLQGLELGADD
                     YITKPFDIDELKLRVQRAIQRADQQSLTDPRSGLPSGKLIEEQLRRIIRLQGWAMMDI
                     RIEHFEAFKEVYGFVAGDDVLRFGAMLMGEVVDEFGSPGDFIGHAGGDNFLLICAEEN
                     ASAIRQSLKNRFANDVLSHYNFMDREQGYIVTYN"
     gene            complement(856..2133)
                     /locus_tag="AC16V7_14850"
     CDS             complement(856..2133)
                     /locus_tag="AC16V7_14850"
                     /inference="COORDINATES: protein motif:HMM:NF012360.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HIR6258449.1"
                     /translation="MLKIILQSPQYIQPFNERARDLRVHNKPLWLFQRDVLSIYTRRE
                     LELPPRAPLPNTSEECLVYRDNLFFDAPYIDAFIKEARRRGRPCQAAFSASDLAFREH
                     ALPLSVSYTPMDDLYLAELWYYPQGVQLESEPLVIDLGASEVGYYHVPIYMAFEQGDL
                     VFQVPRRGLLAIDSWVHIFIADVVFGVFSRGARFEDRLKDDPLFKLRILARAMYEGKQ
                     VLQSSELVKVGRNCVIDPHAVIHGPTTIGDNVTINAGSVVENCIIGDNVNISQGCHLM
                     LSVVGDGAFLPFRSSLFMTTLMDNSMVAQNTCLQMSVIGRNTFIGAGSTFTDFNLLSI
                     PLRALDGNSLLNHANRPVLGGCVGHNCRIGSGMIVYPARTIESDVVLLATSERYVITQ
                     DVRYEDSDHHRLPFGEVHPRLYPRQAEAASESW"
     gene            complement(2134..2745)
                     /locus_tag="AC16V7_14855"
     CDS             complement(2134..2745)
                     /locus_tag="AC16V7_14855"
                     /inference="COORDINATES: protein motif:HMM:NF014845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V4R domain-containing protein"
                     /protein_id="HIR6258450.1"
                     /translation="MHNYSNKMGRIVLVAIEEILGRNGINATLNLAGLSHYINSYPPN
                     NLDLQFNFDDLVEIQVALEELYGPLGGNGIALRSGRASFKYFLREFGPPLGITDVSFR
                     LLPLNEKLSTGAQLFAEIFNRYSDQRVRVEDTQERVYWHVERCPICWGRQTDHPVCHF
                     IVGFLQETLYWVSAGKYFIVEETKCVAMGDPGCTIEINKQPVD"
     gene            complement(2766..>3362)
                     /locus_tag="AC16V7_14860"
     CDS             complement(2766..>3362)
                     /locus_tag="AC16V7_14860"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6258451.1"
                     /translation="VQDFVSLVDQFMEQLGIAQAPLVGHSMGGTVSLSVAIQYPQRVS
                     NVVVIGSPINGSSLNFLLKLFGYRLVAYIVHNNLWILRLGFRLLAPLYTRDKRWPEMM
                     DRDLSKTTLESFLMSIATLRRTDLRPSLDRIRVPVMGMYGDKDIVVDPGQWKPLLEGA
                     PNTRVERFKDAGHFIMLDEPKKFVETIQDFLSCETSSP"
BASE COUNT          847 a          926 c          858 g          731 t
ORIGIN      
        1 tcgttatagg tgacaatata tccctgctcc ctatccatga agttgtaatg cgagagcaca
       61 tcgttagcaa atcggttctt taacgattgc cggatagctg aggcgttctc ttcagcacag
      121 ataagcagga aattatcgcc cccggcatga ccaatgaagt cgccaggtga gccaaactca
      181 tccaccacct ctcccataag catagcgcca aatcgcagaa catcgtcgcc ggcaacaaac
      241 ccatagacct ctttgaaggc ttcaaagtgc tcaatgcgga tgtccatcat tgcccaacct
      301 tgaagccgga taatacgccg cagctgctct tcgattaact tccccgaggg taggccggag
      361 cggggatcgg tgaggctctg ctggtcggcg cgctggatag cacgctgcac acgcagcttt
      421 aactcatcaa tgtcgaaggg tttagtgata tagtcatcgg cgcccagctc taatccctgc
      481 aatttatcgc tacgctcgtc tttctgagtt aaaaagatga taggcacatg gctggtgcgc
      541 gtattggtgc gcaggatacg gcacacctcg aagccatcga tgtcaggaag catgatatcc
      601 agcacaatga ggtgaggcaa gttttggcgg gtcttttcca gtgcatcgct gccgcgcgcg
      661 gcaatatcca cctcataatt caggccatta aagaagatac gcaacatggt ggagatgtct
      721 gtatcgtctt ctacaatgag caaccggcct tttgccatat gagcctccca atatattgtt
      781 tgccttccgg ttcaagcggg aaacgtccca atttggcata tcccgaattc cccggaggag
      841 atattgttct tttacttacc aggattcact ggccgcctca gcctggcgcg ggtaaaggcg
      901 agggtgcact tcaccaaaag gcagcctgtg gtgatcgcta tcttcatagc gcacatcctg
      961 cgtgatcaca tagcgctcgc tcgtagcgag taaaaccacg tccgactcga tggttcgggc
     1021 gggataaacg atcatccccg agcctatacg gcagttatgc cccacgcagc cacccaatac
     1081 tggacggttg gcatggttca gcaggctatt gccatccagg gcgcgcaggg ggatggaaag
     1141 caggttgaaa tcggtgaagg tcgaaccagc cccgataaat gtgttccgcc caatgacgct
     1201 catctgcaag caggtattct gggctaccat gctattgtcc atcagtgtcg tcataaaaag
     1261 cgaggaacgg aaaggcaaga acgccccatc ccccaccacc gatagcatca agtggcaacc
     1321 ttgagagata ttaacattgt caccaataat gcagttttcc accaccgacc cggcgttgat
     1381 ggtcacatta tcgccaatcg tggtcggacc atggataacc gcgtgcggat cgatcacgca
     1441 gttccgaccc accttaacca attccgaaga ctgaagcact tgcttcccct cgtacatggc
     1501 gcgcgccagg atacgcagct tgaagagcgg atcgtccttc aggcgatcct caaaacgcgc
     1561 cccacgcgaa aaaaccccaa agaccacatc tgctatgaat atatgcaccc aagagtcgat
     1621 ggcgagcaaa ccacggcgcg gaacctgaaa gaccaggtcg ccctgctcaa aagccatata
     1681 gatgggcaca tgatagtacc ccacctcgct cgctcccaga tcgatcacca gcggctcgga
     1741 ttcgagctgc acaccctgcg ggtaatacca taattctgcc aggtacaagt catccatcgg
     1801 ggtataagag acagacaagg ggagagcatg ctcccgaaaa gcaaggtcac ttgctgaaaa
     1861 cgccgcttgg caagggcgtc cgcggcgtct agcttccttg ataaaagcat cgatgtacgg
     1921 ggcatcgaag aacagattat cgcgatatac caggcactct tcggatgtat tgggcagagg
     1981 agcccgaggg ggcagttcca attctcgacg ggtataaata gaaagaacat cccgctgaaa
     2041 cagccagagt ggcttattgt ggacacgcag atcgcgagca cgctcgttga agggttggat
     2101 atactggggg ctctgcagga tgatctttaa catctaatct acgggttgct tattaatctc
     2161 gatggtacag cctgggtcgc ccatggcgac acactttgtc tcttccacaa taaaatattt
     2221 gcctgcactg acccaataca gggtctcctg gagaaagcct acgatgaagt ggcacaccgg
     2281 gtgatcggtt tggcgccccc agcagatcgg acaacgctct acatgccagt acacgcgctc
     2341 ttgcgtatct tctacgcgca cgcgctggtc actatagcgg ttgaatatct cagcgaagag
     2401 ctgcgcgcca gtgctcagct tctcattgag cgggagcaag cggaaagata catcggtgat
     2461 gcccaacggc gggccaaact cgcgcaagaa gtatttaaag gaggctcgcc cggagcgcag
     2521 cgcgatgccg ttgcctccta agggaccata taattcttcc aaggcgacct ggatctcaac
     2581 caaatcgtcg aaattgaatt gcaggtccaa attattgggc gggtaactat tgatgtagtg
     2641 cgagaggccg gccaggttca gggtagcgtt gatgccgttg cggccaagta tctcctctat
     2701 cgctacaagg acaatccgcc ccatcttgtt cgaatagtta tgcatcaaaa tgttcgcttc
     2761 tcctgtcatg gacttgatgt ctcgcaactc agaaaatcct gtatggtctc gacgaacttt
     2821 ttgggctcat ccagcatgat aaagtgtcct gcatccttga atcgttccac acgagtgtta
     2881 ggtgcaccct ccaacaacgg cttccattgt cctgggtcaa cgacgatatc tttatcgccg
     2941 tacataccca tcacggggac ccggattcga tccaggcttg gacgcaaatc ggtgcggcgc
     3001 agggtggcaa tgctcatcag aaatgattcg agggtagtct tggacagatc tcgatccatc
     3061 atctccggcc agcgcttatc ccgcgtgtac aggggggcca gtaagcgaaa gcccaagcgc
     3121 aatatccaca aattattgtg cacgatataa gccaccagcc ggtaaccgaa caatttcagc
     3181 aggaaattga gcgatgaacc gttgattgga gagccgatga caacgacatt gctcacgcgc
     3241 tgcggatact ggatagccac cgacaggctg accgtcccgc ccatgctgtg ccctaccagc
     3301 ggggcctggg caatgcctaa ttgttccatg aactggtcta ccaggctaac aaagtcctgc
     3361 ac
//
LOCUS       DBNHOH010000827         8435 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_629765, whole genome shotgun sequence.
ACCESSION   DBNHOH010000827 DBNHOH010000000
VERSION     DBNHOH010000827.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8435)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8435)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8435
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_629765"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="AC16V7_14865"
     CDS             complement(<1..442)
                     /locus_tag="AC16V7_14865"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR6258452.1"
                     /translation="MLVSRLLVAIILVPIALLAISYGGLPYNLLVVLILVLASWEYTN
                     LFRAGGLQPAGFLILAGTLLLGLGRIWDGFGSASWMASLFILASMSIHLIAYERGRDQ
                     AGTDFAVTLAGIFYFGWIGAYLISLRDLPEGKWWVLLALPAVWLA"
     gene            complement(432..1181)
                     /gene="uppS"
                     /locus_tag="AC16V7_14870"
     CDS             complement(432..1181)
                     /gene="uppS"
                     /locus_tag="AC16V7_14870"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HIR6258453.1"
                     /translation="MMKEKNPVFLPEKIPTHIAIIMDGNGRWALSRGLPRLAGHRAGT
                     ENLRRVIEACIEFGIRYLTIYAFSTENWGRPQEEVQGLMRILEDVIDRELQELHDQGA
                     QLHHLGRLDRLHPSLRKKVLYAVDFTKHNSRLILNVAFNYGGRDEIVCAIQRMIEEGV
                     KADDVTDVMVSKHLFTAGVPDPDLIIRTSGELRGSNFLIWQGAYSEWYFTPTYWPDFD
                     KDALLQALHEFSSRDRRYGGVDLKDTAAHAR"
     gene            complement(1197..1754)
                     /gene="frr"
                     /locus_tag="AC16V7_14875"
     CDS             complement(1197..1754)
                     /gene="frr"
                     /locus_tag="AC16V7_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIR6258454.1"
                     /translation="MLKDIYKEAEERMKGAIKALEEDLNGIRTGRASPVLIERLQVDY
                     YGNPTPLIQLASISVPEPRSLLIRPFDMSTLKAIERAILASDLGLTPNNDGKTIRLNL
                     PPLTEERRRDLVKVVHNRVEEARVAARNVRRDSIKDLREFEQAKMISEDERKNGEDEL
                     QKLTDRLIDEINHVGDQKEKEILEV"
     gene            complement(1866..2585)
                     /gene="pyrH"
                     /locus_tag="AC16V7_14880"
     CDS             complement(1866..2585)
                     /gene="pyrH"
                     /locus_tag="AC16V7_14880"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIR6258455.1"
                     /translation="MENSLKYHRILLKLSGEALAGPQGFGIDPRRAEDIAKRVGEVRK
                     LGVDVAIVIGAGNVWRGRVGIERGMERATADYMGMLATVMNALALMDAMERIGLVTRV
                     QSAVEMHAVAEPYIRRRAIRHLEKGRVVILGGGTGNPFFSTDTAAALRAMEIGAEVLI
                     KATKVDGVYDSDPMINPDAVRFERLTYMDSINRRLDVMDSTAISLCMDNHLPILVLNL
                     WQTDVLRQAVFGEEVGTLITE"
     gene            complement(2644..3249)
                     /gene="tsf"
                     /locus_tag="AC16V7_14885"
     CDS             complement(2644..3249)
                     /gene="tsf"
                     /locus_tag="AC16V7_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIR6258456.1"
                     /translation="MAVSIEQIKARREATGAGIMDCRKALEASDGDYEKALEILREKG
                     IATAAKRADREASDGVVELYSHGNGRVGVMVEVNCETDFVARSEAFRTFAHELALQIA
                     AGAPQFIRQEDIPAEVLDLEVEKARQLAKEEGKPDAVIERIIEGKLEKYKNEVVLLRQ
                     LYIRDDSLTIEKLLMQNIGSIGENIIIRRFARWELGETTAN"
     gene            complement(3252..4235)
                     /gene="rpsB"
                     /locus_tag="AC16V7_14890"
     CDS             complement(3252..4235)
                     /gene="rpsB"
                     /locus_tag="AC16V7_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIR6258457.1"
                     /translation="MAVIAMKSLLESGVHFGHRTHKWHPAMKPYIFTERNGIHIIDLQ
                     KTVKALEQSYNLVRDTVAAGSSVLFVGTKRQAQETIKEDAIRAGMPYVTERWLGGMLT
                     NWRTIRQRVNELDRLERMRDKGDFERITKKEALLLSRKIERLDTLLGGIRNMNRLPEM
                     VFVVDVRREETAIHEANLLNIPVIALVDTNCDPRNVDYVIPSNDDAIRAIKLLVGRIA
                     DAAQEGLALRKEEVVEEMPAMAPMGAEEVELSDEELLGEATLAKLTSRTTFIAAEDED
                     VDIEIEEEEEVQEDGEEELEEEDWVEEEEDEVSGKHFEDEDEEEFEDEDEE"
     gene            4704..5447
                     /locus_tag="AC16V7_14895"
     CDS             4704..5447
                     /locus_tag="AC16V7_14895"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR6258458.1"
                     /translation="MRYFATVGSTNDLATRWVEASAPDLSLVVADEQTRGRGRLNRRW
                     FTPPGAALALSLILRPGVGEQDAFSPYFYTALGALATCDALPPQVRAQIKWPNDVIIT
                     QDLDPGKLAGVLAEAHWQGQELAAIILGIGINVAPSSVPPDEQLHYPATCVEAVVKAK
                     IDRWSLLHAVLERLLYWRERLSTQEFLAAWDARLAFRGEWVRVSFADQPPVEGQILGL
                     TAEGRLQLRDRQGVLHELTGGELNVRKGV"
     gene            5449..5652
                     /locus_tag="AC16V7_14900"
     CDS             5449..5652
                     /locus_tag="AC16V7_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258459.1"
                     /translation="MLDDFRQQADEITFEEEDYQPPARPKKYFLGMTPFQRFVIATML
                     LVITCVLSSFALLVTEKVVIPGF"
     gene            complement(5691..8177)
                     /gene="gyrA"
                     /locus_tag="AC16V7_14905"
     CDS             complement(5691..8177)
                     /gene="gyrA"
                     /locus_tag="AC16V7_14905"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR6258460.1"
                     /translation="MEIGNIQQVDIDDQMRAAYLDYAMSVIVARALPDARDGLKPAHR
                     RILYAMHDMGLRSNSAYKKSARIVGEVLGKYHPHGDAAVYDTMARLAQDFSMRYPLVD
                     GQGNFGSIDGDAPAAMRYTEARLAPIAEEMLVDIDKDTVDFTTNFDDSLEEPVVLPAR
                     LPNLLLNGASGIAVGMATNIPPHNLGELASALIYLIENYDKIDDIPVEELIKFIPGPD
                     FPTGGVIVGSEGIWQAYSTGRGRIVLRGLAHIEEMSGGRYRIVVTEIPFQLNKSSLIE
                     RIAELVREGKLDEISDLRDESDRRGMSIIIELKRSAQPKKVLNQLFKYTPLQSTFGVQ
                     LLALVDGEPRLLSLKRALQIFVEHRRVVITRRSQFELQKARARAHILDGLLIALANLD
                     DVIQTIRSSPDADTARERLMFRYLLSELQAQAILDMQLRRLAALERQKIEEEHKKVLD
                     NIAYLEDLLANPGKILDVIKSDLSEISGKYGDQRRTHIAHDANEDFSEEDLVPDEAVL
                     ISITQRGYIKRVMAKAFRAQGRGGRGVTGHATREEDEVLMLVPARTLNTLLFFSDRGK
                     VYSEKAYQIPDADRTGRGIPIVNVLSLDPGETVTAMVAVPDFDAAEYCIMATRDGRVK
                     RVVLSEFSAVRPSGLIAITLDEGDELGWVRLTHGGDEVILVTEQGQALCFSEDQVRPM
                     GRMAAGVNGIKLGKNDKVASMEVVEPDGDLLVVTVGGFGKRTPMKDYPRKRRATGGVH
                     TIDKKALGKVGVVTAARAVQEADDLTIISAGGVVLRTRVQAISRSGRATRGVVLMNLE
                     EGDFVASLARIAAADLRRVGADNGGNAGND"
BASE COUNT         1816 a         2512 c         2205 g         1902 t
ORIGIN      
        1 cggccagcca caccgccggc agcgccagca aaacccacca cttgccctcc ggcaggtccc
       61 gtagggagat caggtatgcg ccgatccagc cgaaataaaa aatgcccgcc agcgtgacgg
      121 cgaagtcggt gcccgcctgg tcccggccgc gctcgtaggc gatcaagtga atagacatgc
      181 tagccaggat aaacaggctg gccatccagg aggcgctccc gaaaccatcc catatccgcc
      241 ctaagcccag cagcagtgtc cccgccagga ttaaaaaacc agcgggctgg agcccgccgg
      301 ctcgaaacag gttggtgtat tcccacgatg ccagcaccaa tatcaagacc accagcaggt
      361 tgtagggcag cccgccatag ctgatcgcca gcagggcgat gggtacgagg atgatagcta
      421 ccagcaggcg gctaacgagc atgcgcggcg gtatccttca ggtcaacccc cccataacgc
      481 cggtcgcgtg acgagaactc atgcagcgcc tgcagtaatg cgtccttatc gaagtcaggc
      541 cagtaagtgg gcgtgaagta ccactcagaa taagcgccct gccagattaa gaagttgctg
      601 ccgcgcagct ctccagaggt gcggatgatc agatcaggat cgggtacacc agccgtaaac
      661 aggtgcttgc tcaccatgac atcggtcaca tcgtcggctt tcaccccttc ctcgatcatg
      721 cgctggatgg cgcacacaat ctcgtcgcgc ccgccatagt tgaaagcgac attaaggatc
      781 aggcgtgaat tatgcttggt gaagtctaca gcgtagagca ctttcttgcg caaggaaggg
      841 tggaggcggt cgagccggcc gaggtgatgc agttgcgccc cctggtcatg cagctcctgc
      901 agctcgcggt cgatcacgtc ctccagaata cgcatcagtc cctgcacctc ttcctgggga
      961 cgaccccagt tctcggtaga aaaggcatag atagtcaggt agcggatacc gaactcaata
     1021 caggcctcga tcacccggcg caggttttcc gtgcccgccc ggtgccccgc caaccgtggc
     1081 aggccgcgcg acaatgccca acgcccgttg ccgtccataa taattgcgat gtgggttggg
     1141 attttctctg gcaggaagac aggatttttt tctttcatca tcacactgag attcggtcag
     1201 acttccagaa tctctttctc tttttgatca ccaacatgat taatctcatc gatgagacgg
     1261 tcggtaagct tctgcagctc atcttcacca tttttacgct catcttccga gatcattttc
     1321 gcttgctcaa actcgcgcag gtccttgata ctgtcacgcc gcacgttgcg agcagccaca
     1381 cgggcttctt caacccggtt gtgcaccacc tttaccaggt cgcggcggcg ctcctcggtg
     1441 agcggcggca agttgagacg gatggtctta ccatcattat taggcgtcaa ccctaggtcg
     1501 gaagccaaga tggctctttc gatagccttg agcgtgctca tgtcgaatgg acggattagc
     1561 aaagagcgcg gctctggcac gcttatactg gcaagctgga tcaggggcgt ggggttgccg
     1621 taataatcga cctgcagcct ttcaatgagc accgggctag cccgccctgt ccggatgccg
     1681 ttcaggtctt cttctaaggc ttttattgct cccttcatgc gctcttcggc ttccttgtaa
     1741 atgtctttca acatgacggc ctcctacccc tataggatac ccgaaaatgt gaagggagaa
     1801 aaggggcaaa tttctgggtt ttcaataggt caagaccccc ttctggtgaa gctctgggga
     1861 caaattcact ccgtaatcag ggtgcccacc tcctcgccaa atacggcctg gcgcaacaca
     1921 tcggtttgcc acaggtttag caccagaatg ggcaggtggt tgtccatgca cagcgagata
     1981 gccgtggaat ccatcacatc caggcggcgg ttgatactgt ccatgtaggt gagccgctca
     2041 aagcgcacag catcaggatt gatcattgga tcgctgtcgt agacaccatc taccttggtg
     2101 gctttgatga gcacctctgc cccaatctcc atggcgcgca gggcagcggc agtatcggtg
     2161 gagaagaagg gatttcccgt gccgcccccc aagatcacta cgcgcccctt ctccaggtgg
     2221 cggatggcgc ggcggcgaat atacggctca gccacagcgt gcatctctac cgcagattgc
     2281 acccgggtca ccaatccaat acgctccatc gcatccatca gggccagagc gttcatgact
     2341 gtcgccaaca tgcccatata gtcggcagta gcgcgttcca tgccgcgctc gatgcccacc
     2401 cggccgcgcc aaacattgcc cgccccgatc acaatggcta catccacacc gagcttgcgc
     2461 acctcgccca cccgcttggc gatatcctct gcccgccggg gatcaatacc gaatccttgc
     2521 ggaccggcca atgcttcacc gctcaactta agcagaatgc ggtgatattt gagagaattt
     2581 tccattgagt tgctccacaa atcagacaca aaaagagcca accggtggtt ggcccttcaa
     2641 caattaattc gctgtcgtct cacccagctc ccagcgcgca aagcgccgga tgataatatt
     2701 ttcaccgatc gagccaatgt tttgcatcag cagcttctcg atggtcagag agtcgtcacg
     2761 aatatagagc tggcgcaata ggacaacctc atttttatac ttctcaagct tcccttcgat
     2821 aatgcgctcg atgaccgcgt ccggctttcc ttcttccttg gcaagctggc gtgctttctc
     2881 tacctcaagg tcgagcacct cggcgggtat atcttcctgg cgaatgaact gcggggcgcc
     2941 tgcagcgatc tgcagggcta actcgtgggc aaaagtacgg aaagcttctg agcgagcgac
     3001 gaagtccgtc tcgcagttga cctccaccat cacgcccacg cgcccgtttc cgtgggaata
     3061 cagctcaacc acaccgtccg atgcttctcg gtcggcgcgc ttggcagcgg tcgcaatgcc
     3121 cttctcccgc aggatttcta gcgctttctc atagtcgcca tccgacgctt ctagagcctt
     3181 acggcaatcc atgatgcccg cgcctgtggc ttcgcgccgc gctttgattt gctctatcga
     3241 aactgccatg attattcctc gtcctcgtcc tcgaattctt cttcgtcctc gtcttcgaaa
     3301 tgctttccgc ttacctcgtc ctcttcctct tctacccagt cttcctcttc cagctcttcc
     3361 tcgccatcct cttggacttc ttcttcttct tcaatctcga tatcgacatc ttcgtcttcg
     3421 gcagcaataa aggtggtgcg gctggttagc ttggcaagtg tggcctcacc caacagttct
     3481 tcgtccgaga gctcaacttc ctctgcaccc atcggggcca tggcgggcat ctcctcgacc
     3541 acttcttctt tgcgcagagc tagaccctcc tgagcggcgt cggctatccg gcccaccagc
     3601 agcttgatgg cgcggatcgc atcgtcgttg gatgggatga cataatccac attgcgcggg
     3661 tcacagttgg tgtccaccag ggcaatgact gggatattta gcaaattggc ttcgtgaatg
     3721 gcagtttcct cgcggcgcac gtccacgaca aagaccatct ctggcaggcg attcatattg
     3781 cggatgccgc ccaaaagcgt atccagtcgc tcgatcttgc ggctcagcaa caaagcttcc
     3841 ttctttgtga tgcgctcaaa atcgcctttg tcacgcatgc gctccaggcg atccagctca
     3901 ttaactcgct ggcggatggt ccgccagttg gtgagcatac cgcccaacca gcgctcagtc
     3961 acatagggca tgccagcacg gatggcgtct tccttgattg tttcctgggc ctggcgtttg
     4021 gtgccaacaa aaagcaccga actcccagcg gcaaccgtgt cgcgcaccag attgtaggat
     4081 tgctccagcg ccttgaccgt cttctgcaaa tcaataatgt ggatgccatt gcgctcggta
     4141 aaaatatagg gtttcatcgc agggtgccac ttgtgggtgc ggtgaccgaa gtgcacgccg
     4201 ctttcgagga gcgatttcat cgcgataaca gccattgggt attctccttt gcgtttaacc
     4261 tccgcctctt tcaacccttc ccggtaccgc gggcaggtag acaacctgcc taatcggcgg
     4321 cacgcccggg aaagtccgga gacgtgtgag atgggatttt gtgccacaaa cgccttcagg
     4381 tgggcacaat tgctcccgcc acaaggcggg agcagaccgg agtatatcat gtggtacagg
     4441 attcgtccag aggaaaattg gcaatcggtt tcggaaatag gaaaaattta tctccattac
     4501 aattttaaca acccagggca attgcatatg ccctgagtgg catgccccca ccaaaacacc
     4561 ccattttctc tcctcagtgg tgggaaatcg cccaaatgcg attgccctat taacaaccta
     4621 agttctcggt ttttatgcgg taaaatcgag atatagacca gactttttta caatcccttc
     4681 tcgccgacct ccccttgggt gcagtgcgtt attttgccac agtcggttct accaatgatc
     4741 tggcaacgcg ctgggttgag gctagtgcac cagatctctc gctggtggtc gctgacgagc
     4801 agacccgcgg gcgcgggcgc cttaatcgtc gctggtttac cccacccggt gctgccctgg
     4861 ctcttagcct gattcttcgg cccggcgtgg gggagcaaga tgcattctct ccctactttt
     4921 ataccgcgct tggggcgctg gccacctgcg acgccttacc gccccaagtt cgagcgcaga
     4981 tcaagtggcc gaacgatgtg atcatcaccc aagacctaga ccctggtaag ctggcaggcg
     5041 tcctggctga agctcactgg caaggccagg aactggctgc cataatcctt ggaatcggca
     5101 ttaatgttgc accttcttcg gtacccccgg acgagcagct ccattaccct gcgacctgcg
     5161 tggaagccgt agtgaaagca aagattgatc gttggagctt gctgcacgcc gtgctagaga
     5221 ggctgcttta ctggcgcgag cggctcagca cgcaggagtt cctggcagcc tgggacgccc
     5281 ggctggcctt tcgtggtgaa tgggtgcggg tttcgtttgc agatcagccc ccggtggaag
     5341 gccaaatact gggattaacc gccgaagggc gcctgcaact gcgcgaccgc cagggcgtgc
     5401 tacacgagct gaccggcggc gagctcaatg tgaggaaagg agtttgagat gctagatgac
     5461 tttcgccaac aggctgacga gataactttt gaggaagagg attaccagcc acccgcccgc
     5521 ccgaagaagt atttcctggg gatgacaccc tttcagcgct ttgtgattgc gacgatgttg
     5581 cttgtgatta cctgcgtgct gtcatccttc gcgctgctgg tgacagagaa ggtagtgatt
     5641 ccaggatttt gagacaaaca caatccttcg tcttgccgat tgattggtta tcaatcattg
     5701 cccgcattgc caccattatc cgcgcccact cgacgcaagt ccgccgcggc gatacgcgct
     5761 agggaagcca caaagtcgcc ttcctctaga ttcatcagca ccacaccgcg cgtcgctcgc
     5821 ccagagcgcg aaatagcctg caccctcgtg cggaggacga caccaccggc ggagatgatg
     5881 gtcaggtcgt cagcttcctg cactgcacgc gccgctgtaa caacccccac tttacctaaa
     5941 gcttttttat cgatggtgtg cacaccacca gtggcgcgcc tcttgcgtgg gtagtccttc
     6001 attggtgtgc gtttaccaaa gccaccaacc gtcaccacca gcaagtcgcc atctggctcc
     6061 accacctcca tactagccac cttgtcattt ttgcccagct tgatgccgtt cacccctgcg
     6121 gccatccgcc ccatcggtcg aacctgatct tcagagaagc ataacgcttg tccctgttcg
     6181 gtgaccaaaa tcacttcatc acccccgtgc gtcaggcgga cccagccgag ctcgtcaccc
     6241 tcatccagtg tgatagcgat caaaccagag ggacgcactg cgctaaattc ggagagcaca
     6301 actcgcttga cgcgcccatc gcgcgtggcc atgatgcagt attcagcggc gtcgaaatcg
     6361 ggtacagcaa ccatggctgt caccgtttcc cctggatcta aggaaagcac attgacaatg
     6421 ggaataccac gcccagtccg gtcggcgtct gggatttggt acgctttctc tgaataaacc
     6481 tttccacggt cagagaagaa gagcagcgtg ttcagagtcc gagccgggac taacatcagg
     6541 acctcatctt cctcacgggt ggcatgaccg gtaacaccgc gcccgccgcg tccttgcgcc
     6601 cggaaggcct tggccatcac gcgcttgatg tagccacgct gggtgatact gatcagcaca
     6661 gcctcgtcag gaaccaagtc ctcttcggag aagtcttcgt tggcatcatg ggcaatgtgc
     6721 gtgcgccgct gatcgccata tttcccggat atctcgctta ggtcgctctt aatgacgtcc
     6781 aggatcttcc cggggtttgc cagcaagtct tccagatagg caatgttgtc caatactttt
     6841 ttgtgttctt cttcaatctt ttggcgctcc agtgcagcca ggcggcgcaa ctgcatatcc
     6901 aggatagcct gcgcctgcag ctcgctcaag agatagcgga acatcagacg ctctctggct
     6961 gtatcagcgt caggcgagct acggatggtc tgaatcacat catccagatt cgccaaggcg
     7021 atgagcaatc cgtccaggat atgggcgcgg gcgcgggctt tttgcaactc aaactgggag
     7081 cggcgggtaa ttacaactcg ccggtgctca acaaagatct gcaaggcgcg cttgagcgat
     7141 agcaggcgag gctcgccatc caccaaggcg agcaactgga caccgaatgt ggactgcaac
     7201 ggggtgtact tgaagagctg gttgagtact tttttgggct gggcgcttcg cttgagctcg
     7261 atgatgatgc tcatgccccg ccggtcggat tcatcgcgca ggtcagaaat ttcatctagc
     7321 ttgccttcac gaaccagttc ggcgatacgc tcgatcaagg aagatttgtt aagctggaaa
     7381 ggaatctcgg tgaccacaat gcggtaacgg cctccgctca tctcctcgat atgggctaga
     7441 ccacgcaaca cgatgcgccc gcgaccggtg ctgtaggctt gccaaatgcc ctcagatcca
     7501 acaatcacgc caccggtggg gaaatctggg ccggggatga actttatcag ttcttccacc
     7561 gggatgtcgt caatcttgtc gtaattttcg attagataga tcaaggcgct tgccagctcc
     7621 cccaggttgt gcgggggaat attggtcgcc atacccacgg cgatgccgga ggcaccgttt
     7681 agcagcaggt ttggcaggcg cgccggcaga acaaccggct cctccagcga gtcatcgaag
     7741 ttggtggtga aatcgactgt gtccttgtcg atatcgacca gcatctcctc agcgattggc
     7801 gccaggcgcg cctcggtata gcgcatagcg gcaggtgcgt caccatcgat ggaaccgaaa
     7861 ttgccctgcc cgtccaccag cggataacgc atagagaaat cctgagccag ccgcgccatg
     7921 gtatcgtaca ccgcagcgtc accatgcggg tgatacttgc ccagcacctc gccaactata
     7981 cgggcggatt ttttataggc gctattcgaa cgcaagccca tatcgtgcat ggcataaagg
     8041 atgcgcctgt gggcgggttt cagtccatcg cgagcatccg gcaaggcgcg cgcaacaatc
     8101 acgctcatgg catagtctag gtatgcggcg cgcatttgat cgtcgatatc tacctgttga
     8161 atgttaccaa tttccattaa aaaatatcct tctatcacaa aaagtgcaaa gccggacaaa
     8221 cagcttaact ataaaattat acctctccaa cagactattt gagcttcgat atatggcgtg
     8281 ccattgagta gctctatgat gtgccacgaa aatattggcc ggattggctg attaaagagc
     8341 gatcagatca tcggcaacct gactcccatc acaaaactaa atacagtgat ctatcacgcc
     8401 atattccaga gtgtctgttt ctccaatctg gggat
//
LOCUS       DBNHOH010000828         9165 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_501352, whole genome shotgun sequence.
ACCESSION   DBNHOH010000828 DBNHOH010000000
VERSION     DBNHOH010000828.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9165)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9165)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9165
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_501352"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="AC16V7_14910"
     CDS             <1..664
                     /locus_tag="AC16V7_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258461.1"
                     /translation="QSGYEGLTMPWIIFYAAILRPSFPLLLIVLVGWVYTLINLAQTY
                     FFYCSRIIFAGSFDRLIPEKVCYIHPTLNSPVIAIAIISVIAEIGVIDAALGGVMGAQ
                     LNFVFFAVCTQLVPVLSMTLFPYLKPDLFENSSAFVRRKLGNIPVITIVGTITMTYLL
                     WMIIASFAFPAVGGRIGTGTLGTLAGFIISGIVVFYIARAYRMRSEGIDIKWTFQSIP
                     PI"
     gene            760..1740
                     /locus_tag="AC16V7_14915"
     CDS             760..1740
                     /locus_tag="AC16V7_14915"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR6258462.1"
                     /translation="MKFGSLFRGSKITRILYGSDFHGSEAVFRKFLSAALQYQASYLI
                     VGGDVTGKAMIPIIHQGNGRYVGYVFGRKEEPSTPEELDKLKKTISNVGFYPIVVEKD
                     EAQALESNKELMSQRFEEEMCQRVRDWMVLAEQTLVPQKKTLYFMPGNDDLYSVDAVI
                     DEFEHVFNPDMKRFIIDDDYELYGLSNANMTPWACARDVEEDELMLKLGALEELVTNP
                     ERAILALHVPPYASNLDTCPELDKDLKMITLGGQVVMKSAGSTAVKAFIEKVQPMLTL
                     HGHIHESPGYTRIGRTLAINAGSEYAEGILKAAIINLEGGKVKGHLLISG"
     gene            1742..2524
                     /locus_tag="AC16V7_14920"
     CDS             1742..2524
                     /locus_tag="AC16V7_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258463.1"
                     /translation="MGKFGDIQEDFYAERTRILEALQEPENRHVIMRLIGALAFRTHC
                     PQFGHIQDELGRKFTDIDFASYRRFIKDIRRLLSELGYLEEKMVTQLFGDARLLFNDP
                     VYDRHIDIFFEQLDFCHPVSFSGRLEVEELTLPLAELLLEKMQIVQINEKDLIDSIML
                     FREHPIGEGDRETVNAEVIASTLGDDWGFWRTVTGNLNLLDQMLDQYTGLKDEDRQVV
                     RERIHQLQVRIESQPKTVKWKLRSRIGERVKWYKDVEELMHR"
     gene            2580..4115
                     /locus_tag="AC16V7_14925"
     CDS             2580..4115
                     /locus_tag="AC16V7_14925"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF012589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HIR6258464.1"
                     /translation="MTPTKKYILAHDTGTGGDKAVITDLQGRVIHSAYQSYGLSYPQA
                     EWVEQDPDELWRTVAATTREVIRQSGVDPAEILGVGISAQMFNALPVDEQGQPLTPML
                     SWLDLRSIRQADRLFQGDLPAFLFQHTGNMPTAKDVIPKILWLKEERPDIWERTAYLF
                     DCKEYIIYKLTGKVATDWVGASCYFLFDPHQRSWSPKACARLGIPLDKLPPAYPCTDV
                     IGEVTEEAAKLTGLLPGTPVVNCGGDVATAQSGAGANRDGKAHLCIGTATWVGISTSE
                     FKNDPEKPLWGLSHLHPKKWVIAGEMETGGGALMWFRDTLFGEQAGQAGGELSTYEMI
                     SKLAQSLEPGSDKLIFLPWLSGERAPVLDHYARGGFVGLTLSHNMAHMARAVMEGVAY
                     HIRWICETLDRLGFQFEEVNAIGGGCKSEIWPQIISDVTGRELHIVEHPLEAGAMAAA
                     LTVAVGMGVYASMDEIDDLIHIKKVVQPIAAHAKRYDGLYQAYRQLYTALSPIFRQMY
                     QIQ"
     gene            4150..4839
                     /locus_tag="AC16V7_14930"
     CDS             4150..4839
                     /locus_tag="AC16V7_14930"
                     /inference="COORDINATES: protein motif:HMM:NF019976.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HIR6258465.1"
                     /translation="MKSIIDQQKIARRASLSNIASIGGLFALLVSVLIPLFYPGYMLV
                     ANLLLFGGLGISMVGIYFANRWVKKPRPEASLDAALKSLSNAHRLYHYPRLPCDHVLL
                     APNGVFVLETINLAGYFIYKRGRWREKMNVGRALRYIVEEHLGDPIKSARSSAQYLEE
                     RLSQNTGEGSQVPVKALVVFSHPVAVIDITAPPIPVCKLDKLKKHLQMQAPRLDQGVY
                     AQIAEYLDSSI"
     gene            5070..5948
                     /locus_tag="AC16V7_14935"
     CDS             5070..5948
                     /locus_tag="AC16V7_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015028310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional helix-turn-helix domain-containing
                     protein/methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR6258466.1"
                     /translation="MVAPAFEDYVQLSEDYNRIEQAIHFLEANYQAQPNLSDVAASVN
                     LSEYHFQRLFTHWVGISPKRFLQFLTKEHAKELLSHSENILDVAYETGLSGPGRLHDL
                     LVTTEAATPGELKSQGEGLVITYGFHPTPFGKCLLALTERGICDLVYIERDDRNEALA
                     LVKGRWPRATLQQDERQTQTVLPKIFSKLAGYETGPLSLYLRGTNFQIKVWEALLRIP
                     YGAVVSYRDIASHLGIPSAARAVGNAVANNPIPVLIPCHRVIRKAGDFGAYRWGSARK
                     KALLGWELAKNANWKE"
     gene            5952..6890
                     /locus_tag="AC16V7_14940"
     CDS             5952..6890
                     /locus_tag="AC16V7_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020481700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR6258467.1"
                     /translation="MKFRIWLALISVYITWGSTYLAIRFAVETIPPFLMAGVRFLIAG
                     TILYAWRRLSGDAPPTRVQWRSAAIVGLFLLLGGNGGVSWAEQYVPSGIAALIVGSAP
                     FWMVLIDALRPGGQRPNWQTASGVLVGFLGIALLINPSQESGGLLEMNVAGVVALLLA
                     SLLWAIGSLYSRTAQLPESPLLGTGMEMLAGSASLFLVGTLAGEWGSLDLNAITPRSL
                     WGMAYLITFGALVGFVSYTWLLRVAPTPLVATYAYVNPLIAVILGNLLAKEILTPQII
                     VAALIIVGAVAMINTGRTRALKPAGQLSVVPVSRED"
     gene            complement(6900..7907)
                     /locus_tag="AC16V7_14945"
     CDS             complement(6900..7907)
                     /locus_tag="AC16V7_14945"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HIR6258468.1"
                     /translation="MDTILIVEDNRDLCEALHEILSFDGYAVITASNGADALGKMQTV
                     SPDLIVSDITMPQMDGFTFFQAVRARPDWLAIPFIFLTARGERDDIIRSKYLGAEDYL
                     IKPLTQEELLTAVRARLDRSQQIRIATLRQAYETSLTVLANAIDVRDVNAQGHVERVV
                     AYSMILATELNLQGRLLDQLRLAAILHDIGKIIIQESTLFKFVSLNVTEMAEIRKHAS
                     AGAEMVRDIEYLTAIAPLIRHHHERWDGGGYPDGLSGENIPLGARIIALADSLDNMVL
                     EKPYKAALTMDQAYEEILNNSGSRYDPAVVAVFQRAWQAGKIHAVRDQWVSTKQEATQ
                     T"
     gene            complement(7910..8908)
                     /locus_tag="AC16V7_14950"
     CDS             complement(7910..8908)
                     /locus_tag="AC16V7_14950"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005581764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HIR6258469.1"
                     /translation="MRRSLAITSIPGLRVGHAQDDEALTGVTVVLCEQGAVGGVDQRG
                     GAPGTRETDAMHPLHLVERVHAIVLSGGSAFGLDSASGVVRFLEERGVGFDVRVARVP
                     IVPAAILFDLGIGRADVRPDATMGYQACLNASANPPAEGNVGAGIGCTVGKILGITQA
                     MKSGLGSAAIDIGKGVIVAAIVAVNVFGDVINPQTGQIMAGARAITDEPLQMGAKGYF
                     ADTLQVMKTMVGRTFGFARRENTAIGVVATNAKLSKEHINKVAQMAQDGLARTVRPAH
                     TMLDGDTIFALSAGSRKADVNIVGAFAAEAFAQAVLRAVQMAQPLAGLPSALGGAL"
     gene            9064..9135
                     /locus_tag="AC16V7_14955"
     tRNA            9064..9135
                     /locus_tag="AC16V7_14955"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9096..9098,aa:Lys,seq:ctt)
BASE COUNT         1986 a         2365 c         2601 g         2213 t
ORIGIN      
        1 ccagtctggt tatgaaggcc tgacgatgcc ctggatcatc ttctatgccg ccattctcag
       61 gcctagcttc ccgttgttgc ttatcgtgct ggtgggttgg gtgtacacgc tgatcaacct
      121 ggcgcaaact tatttcttct attgcagccg gatcattttt gccgggtcct ttgaccggct
      181 aatcccggag aaggtttgct atatccaccc cacgctgaat agcccggtca tcgccatcgc
      241 catcatctcg gtgatcgccg agataggtgt gatcgatgcg gcgttgggcg gggtgatggg
      301 tgcccagctc aactttgtgt tctttgccgt gtgtacgcaa ctggttccgg tactatcgat
      361 gacgctcttc ccttacctga aaccggatct gttcgagaac tccagcgcct ttgtacggcg
      421 caagctgggc aatatcccgg ttattaccat cgtagggacg attacgatga cctacttgct
      481 gtggatgatc attgcttcct ttgctttccc agcggtgggt gggcgtatcg gcacgggtac
      541 tttgggtacg ctagccgggt tcatcatcag cggaatcgtc gtcttctata tcgcacgtgc
      601 ttaccgcatg cgatcggagg gcatcgatat caaatggacc ttccagtcca tcccgcccat
      661 atagacttgg cgcttaccct cacctaatct ggccttcccc tctccacggg ggagagggga
      721 agtcaggtgg gggcgaaaga acttatacgg agtatattaa tgaaatttgg tagcctgttt
      781 cgcgggtcga aaattacgcg tatcctgtat ggttccgact tccatggatc tgaggcggtt
      841 tttcgcaagt tcctctcagc ggcacttcag tatcaagcca gctacctgat cgttggtgga
      901 gatgtcactg gcaaagcgat gattcccatc atccaccagg gaaatggtcg ctacgtgggc
      961 tatgtctttg gtcgcaaaga agaaccgtcc acgccggaag agcttgataa acttaaaaag
     1021 accatcagca atgttggttt ttatcccatc gtggttgaga aagacgaggc gcaagcgctg
     1081 gaatcgaaca aggagcttat gagccagcgt tttgaagagg agatgtgcca gagagtgcga
     1141 gattggatgg tgctggctga gcagacactc gtgcctcaaa agaaaaccct ctatttcatg
     1201 cctggtaatg atgatctata ctctgttgac gcagtcatcg atgagtttga gcatgtcttc
     1261 aacccggaca tgaagcgatt tattattgat gatgactatg aattgtatgg cctctcgaat
     1321 gccaatatga ccccctgggc ttgcgctcgt gatgtagagg aggacgagct gatgttgaag
     1381 ctgggtgcgc tggaagaatt ggttaccaac ccagagcgag ctatccttgc cctacacgta
     1441 cctccttatg cttcgaacct ggatacctgc ccggagctgg ataaagattt gaagatgatc
     1501 acactgggag gtcaggtggt gatgaagtcg gctggctcca ccgcggtgaa agcctttatt
     1561 gagaaagtgc aacccatgct gaccctccat gggcatatac atgaatctcc gggatataca
     1621 cgtatcggtc gcacgttggc gatcaacgct ggcagcgaat acgcggaggg tatcttaaaa
     1681 gcagctatca ttaacctgga gggtggcaag gtcaagggac acttgctgat ttctggataa
     1741 tatgggtaaa ttcggagaca tccaagagga cttctacgcc gagcgcacgc gtatcctgga
     1801 ggcgctccag gaaccagaaa accggcacgt catcatgcgc ctgattgggg cgttggcttt
     1861 ccgcacccat tgtccgcaat ttggtcatat tcaggatgaa ctgggacgca agtttaccga
     1921 tatcgatttt gcttcttacc gccgctttat aaaggatatc cgccgcctgc tcagcgagtt
     1981 gggctacctt gaagagaaaa tggttaccca gctctttggc gatgcgcgcc tgcttttcaa
     2041 tgaccctgta tatgacaggc acatcgatat tttcttcgaa caacttgatt tctgtcaccc
     2101 ggttagcttc tccggtcgat tggaggtgga ggaactgaca cttcctctgg ctgaactgct
     2161 gctggagaaa atgcagatcg tacaaatcaa tgagaaggac ttgattgatt ctattatgct
     2221 ctttcgcgag catcctattg gcgaaggtga ccgggaaacg gtaaatgctg aggtgattgc
     2281 ttctacccta ggtgatgact ggggcttctg gcgcactgtc actggcaatc taaacctgct
     2341 ggaccagatg ctggatcaat acactggtct aaaagacgag gatcgccagg tcgtgcgaga
     2401 acgcatacac cagttgcagg tgcgcattga aagccagccg aagacggtaa aatggaagct
     2461 gcgcagccga attggcgagc gagtgaagtg gtacaaagat gtagaggaat tgatgcaccg
     2521 ctgacacgct tcgccggata tttgaaaatg atcgattgtg gcatacttaa ggattctcaa
     2581 tgacgcccac aaagaaatac atcctggcgc acgacactgg aaccgggggt gacaaagctg
     2641 tcatcaccga ccttcagggt cgggtgattc actcggcata ccagtcgtac ggcttgagct
     2701 atccccaggc agagtgggtg gagcaggacc ctgacgagct gtggcgcacc gtggctgcca
     2761 caactcgaga ggttatccgg caatcgggtg tggacccggc ggagattcta ggggtgggca
     2821 tatctgccca aatgttcaac gccttgcctg tggatgagca aggccaaccg ctgacaccaa
     2881 tgttgagctg gctggacctg cgcagcatcc gccaggctga tcgcctgttt caaggtgact
     2941 tgcctgcctt tctcttccag catactggca acatgcccac cgccaaagat gtgattccta
     3001 aaatattatg gctcaaagag gagagaccag acatctggga gcgcaccgct tatctttttg
     3061 actgtaaaga atacattatt tataaactga ccggaaaagt agccaccgac tgggtggggg
     3121 cttcctgtta cttcttgttc gatccgcacc agcgcagctg gtcgccgaag gcctgcgctc
     3181 gcctcggcat tccactggat aagctgccgc ccgcctaccc ctgtactgat gtaatcgggg
     3241 aggttactga agaggcggca aagctgaccg gcttattacc tggcacaccg gtagttaact
     3301 gtggcggaga tgtggctaca gctcaatctg gggcaggtgc gaaccgggat gggaaagcgc
     3361 acttgtgcat tggcactgcc acttgggtgg gtatctcaac ctccgaattt aaaaatgacc
     3421 ccgaaaagcc attgtggggg ctgagccacc tgcatcccaa gaaatgggtc attgcaggtg
     3481 agatggaaac tgggggcggt gcgctgatgt ggtttcggga tacgcttttt ggggagcagg
     3541 ctggtcaagc tggcggcgag ctttcaacct acgagatgat cagcaagctc gcccaatccc
     3601 tggaaccggg ctctgataag ctgattttct tgccatggct ctccggagag cgagctccgg
     3661 tgctggacca ttatgcccgg ggcggctttg tcggattgac cctgagccac aacatggctc
     3721 acatggcccg cgccgtgatg gaaggcgtag cctaccatat ccgctggatc tgcgagacct
     3781 tggatcgtct gggctttcaa ttcgaggaag ttaacgccat tggtggtggc tgtaagagtg
     3841 aaatttggcc gcagatcatc agcgatgtca ccgggcgtga gctccacatt gtggagcacc
     3901 cgctggaggc gggtgccatg gctgctgccc tgactgtggc ggtggggatg ggggtctacg
     3961 ccagcatgga tgaaatcgat gatttgatcc atatcaagaa ggtcgtgcag cctatcgctg
     4021 ctcatgccaa gcgctacgat gggttgtatc aggcttaccg gcagttatac actgccttat
     4081 cacccatatt ccggcagatg taccagattc aatgatttat ctattcacat ggaatggcag
     4141 gctaccgcca tgaaaagcat catcgaccag caaaaaattg cccgccgggc gtcgctctct
     4201 aatattgcca gtattggggg cttgttcgcc ctgctggtca gcgtgctcat accgctcttt
     4261 tatcctggat atatgctggt tgctaacctg ctgctgtttg gcggtttggg catctccatg
     4321 gtgggtattt attttgccaa tcgttgggtt aaaaagccac gcccagaggc cagcctggat
     4381 gcagcgctta agtcgctcag caacgctcac cgcctgtacc actatcccag gctgccttgc
     4441 gatcacgtgc tgctcgctcc caacggcgtg ttcgtcctgg agacgataaa tctggcaggt
     4501 tatttcatct ataagcgtgg tcgctggcgc gagaagatga atgtgggtag ggcgcttcgc
     4561 tatattgtag aagagcacct gggtgacccg atcaagagtg ctcgcagctc agcgcagtac
     4621 ctggaagaac gactctctca aaacacgggg gaggggtcgc aggtgcccgt gaaggcgctg
     4681 gtggtattct cccatcccgt tgcggtgatc gatatcacag ccccacccat cccggtctgc
     4741 aagttggata agttaaaaaa acacctgcaa atgcaggcac cgcgtctgga tcagggggta
     4801 tatgcacaga tagcagaata tttggacagc agtatataac cttccataac gccagtcgtt
     4861 cgtccgctag cgaatatgga ttaagttagc cagggggata tgccatttat gtcttgcaag
     4921 ccccccataa cctgtaattc taagatgaat attgccgaga tagcaagaat cgggtggtgc
     4981 ttagcggaga gagcatctat aatctgggaa attatggtaa aggcgtaacc tgcctcgcgt
     5041 atttgtttct attattggag gaaacaccca tggttgcacc agcgtttgag gattatgtcc
     5101 agctctccga ggactacaat cggattgaac aagcgattca cttccttgaa gctaattacc
     5161 aggctcaacc caacctgtcc gacgtggctg ccagtgttaa cctgagcgag taccactttc
     5221 aaaggttgtt cacccactgg gtgggcatca gtccgaagcg ttttttgcaa tttctgacca
     5281 aagaacatgc caaggagctt ttgtcccatt cggaaaacat cttggatgtg gcttatgaga
     5341 ctggcctgtc tggcccgggg cggctgcacg acctgctcgt caccactgag gcggcgaccc
     5401 ccggcgagtt gaagagccag ggcgaggggt tggtcatcac ctatggattt catcccacac
     5461 cttttggcaa gtgcttgtta gcccttacgg agcggggcat ctgcgatctg gtctatattg
     5521 agcgggatga ccgtaacgag gcgctggcgc tggtcaaagg ccgttggccg cgcgcaaccc
     5581 tgcagcagga cgagcgccag acacagaccg ttttgcccaa gatcttctcg aagttggcgg
     5641 gatatgagac gggaccgctt agcctgtact tgcgcggcac taattttcaa ataaaagtct
     5701 gggaggctct tttgcgcatt ccgtacggcg ctgtggtatc ataccgggat attgccagcc
     5761 acctgggtat accctccgcc gcgagagccg tgggcaatgc cgtcgcaaac aatccaattc
     5821 ctgtgcttat tccctgtcat cgtgtcatcc gcaaggcagg ggattttggc gcttaccgtt
     5881 ggggttccgc tcgaaagaag gcattacttg gatgggagct ggcaaagaat gcaaactgga
     5941 aggaatagtg gatgaagttt cgcatctggc ttgctttaat ttcggtatat atcacctggg
     6001 ggtctaccta ccttgcaatc cgttttgcgg tggagaccat cccgccgttt ctcatggctg
     6061 gggtgcgctt tttgatcgcc gggactatcc tgtatgcttg gcgccggctt tctggagacg
     6121 ctccgccaac ccgcgtgcag tggcgctcag cggcgatcgt cggccttttc ttgttgttag
     6181 gcgggaacgg tggggtttct tgggccgaac agtacgttcc ttcgggaata gcagcactga
     6241 tcgtgggatc agccccgttt tggatggtgt tgatagatgc cttgcgccca ggtggacagc
     6301 gacccaactg gcagactgcc agcggcgtgc tggtgggctt tttgggtatt gccctattga
     6361 ttaacccatc tcaggaaagt ggtgggttgt tggagatgaa cgttgcgggt gtcgttgcgt
     6421 tattgcttgc gtccttgtta tgggcgattg gatcgcttta tagccgcacg gcgcaactgc
     6481 cagaatcgcc gctgcttggc actggcatgg aaatgctggc aggtagcgcc agcctgttcc
     6541 tggttggtac gctggctggc gaatggggca gcctggactt gaatgccatt acgccgcgct
     6601 cactatgggg aatggcttat ctgatcacct ttggggcgct ggtgggcttt gtatcctata
     6661 catggctgct gcgcgtcgcc ccgacgcccc tggtggctac ctatgcgtac gtgaatccgc
     6721 ttatcgcggt gatcttgggc aacctgttgg caaaagaaat acttaccccg caaatcatcg
     6781 tagccgcgct gatcatcgtg ggcgcggtgg cgatgattaa caccggacgc accagggcgc
     6841 tcaagccagc agggcagcta agcgttgtac cagtatctag agaggattag gtgggattat
     6901 tatgtttggg ttgcttcttg cttcgtagat acccattgat cacgcacggc gtgaattttg
     6961 ccagcttgcc aggctctttg aaagactgcc accaccgctg ggtcatagcg gcttccagag
     7021 ttgttcagga tttcctcata ggcctgatcc atggtcaggg cggccttgta aggcttctcg
     7081 aggaccatat tgtctaggct atctgccagg gcgataatgc gcgcccccag ggggatattc
     7141 tctccagaga gaccgtcagg gtatccaccc ccgtcccagc gctcgtgatg gtggcgtata
     7201 agcggggcga ttgcggtaag gtattcgata tctcgaacca tctctgcacc agccgaggca
     7261 tgtttcctaa tttccgccat ctctgtcacg ttcagggata caaatttgaa taaagtcgat
     7321 tcctggatga tgattttgcc gatatcgtgc aaaatagccg ccagacgtaa ttggtccaac
     7381 aagcgccctt gcagattcaa ttcagttgct aaaatcatcg agtaggcaac cacccgttct
     7441 acgtgccctt gggcattgac atcgcgcaca tcaatggcgt ttgccaatac ggttaagctg
     7501 gtctcgtagg cttgtctcag ggtggcaatg cgaatttgct gagagcggtc cagcctggcg
     7561 cgcactgcgg tgagcaattc ttcttgcgtg agcggcttga taaggtaatc ttctgcgccc
     7621 aggtatttgc ttcggatgat atcatctcgc tcgccgcgag cggtgaggaa gataaagggg
     7681 atcgccagcc aatccggccg ggcgcgcacg gcttggaaaa aggtgaaacc atccatctgg
     7741 ggcatggtaa tatccgatac gatcaggtct gggctgacgg tttgcatttt tcccaatgca
     7801 tccgccccgt tggaggcggt gatgaccgca tagccatcaa agctcaagat ttcatgcaga
     7861 gcttcacaca gatctcggtt atcttcaacg attagaatag tatccatggt cataatgctc
     7921 cccctaaggc ggaaggcaat ccagcgagcg gttgagccat ttgcacggcg cgcaagactg
     7981 cctgggcaaa ggcctcggca gcaaaagcgc ccacaatgtt aacgtccgct ttacggctcc
     8041 cagcggagag ggcgaagatg gtgtctccat ccagcatggt gtgagccggg cgtacggtgc
     8101 gggccagccc gtcctgtgcc atctgggcga ctttgttgat atgctctttg ctcagcttag
     8161 cgttggtggc gaccacgcct atggctgtgt tttcccgccg ggcgaagcca aaggttcgcc
     8221 caaccatggt tttcatcacc tgcagcgtgt cggcgaagta gccttttgca cccatttgaa
     8281 ggggctcatc ggtgatcgcc ctggcgcctg ccatgatctg cccggtttga gggttgatga
     8341 catcgccgaa gacgttcacc gcaacgatag cagccacgat cacgcctttg ccgatgtcga
     8401 tggcagcgct gcccaggcct gatttcatgg cttgggtaat ccctaatatt ttgcctacag
     8461 tacagccaat accggcaccc acattgcctt cagcgggggg atttgctgag gcgttcaggc
     8521 aggcctggta tcccatggtt gcatcagggc gcacatcggc gcgaccgatg cccaggtcga
     8581 agaggattgc tgcggggacg atcggcacgc gggctacgcg gacatcgaag cccactcccc
     8641 gttcttctag aaaacgcacc acaccgctgg ccgagtctaa tccgaaggcg gagccgcctg
     8701 agagcacgat agcgtgcacg cgctcgacca ggtggagcgg gtgcatagcg tcagtttccc
     8761 gcgtgccggg agcgccgccg cgttggtcca cgccacccac agcaccctgc tcacagagca
     8821 ccacggtgac accggtgagc gcttcatcgt cctgggcatg acccacgcgt agcccaggta
     8881 tggaggtgat ggccaaagat cgtctcaaag ttacatccgc tgatcagtca agttttggct
     8941 tgcggtctat aattatatag gcaaaaagcc cgccccctcc ttacaagtcc gtcaactccg
     9001 ggcggaattg agcccatggg tttgtttgac aagacttccc tttggggtat aattctcccg
     9061 cacggccccg tagctcaatg gtagagcagt tgactcttaa tcaattggtt acaggttcga
     9121 gtcctgtcgg ggtcacctag atataagggt atttgccagc gaaac
//
LOCUS       DBNHOH010000829         2487 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1206324, whole genome shotgun sequence.
ACCESSION   DBNHOH010000829 DBNHOH010000000
VERSION     DBNHOH010000829.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1206324"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            505..981
                     /locus_tag="AC16V7_14960"
     CDS             505..981
                     /locus_tag="AC16V7_14960"
                     /inference="COORDINATES: protein motif:HMM:NF046293.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR6258470.1"
                     /translation="MSKIDKKDLQIVNLLMEDGRMSAAEIARQVGNISERVVRYRIDR
                     MVKEGIISINAIPKPKALGFTVIADVFIEVETGRIQEVATKLKDMECVSYVACAIGET
                     DISVQLVAHDTGEVYQFVTEVIGRTPGVRKTTTSIVPYTLKDVYQWRIPPSVSVDD"
     gene            1028..2332
                     /gene="gntE"
                     /locus_tag="AC16V7_14965"
     CDS             1028..2332
                     /gene="gntE"
                     /locus_tag="AC16V7_14965"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanitoxin biosynthesis PLP-dependent
                     transaminase GntE"
                     /protein_id="HIR6258471.1"
                     /translation="MAIQREKSNSLQRRAMAVTPLGVNSNFRYWGDGITPYVLKAKGG
                     HLWDVDGNRYIDYRLAYGPIILGHAYDEVDAKVHQEIDRGVLFAMTSELEIAVIEKIV
                     EMCPGVEMARLACSGTEATMHAIRVARAYTGREKIIKFEGMYHGFQDYTLFSTYAPVE
                     AYGNYRSPIPIPASSGIPRALHDLVITLPFNEPEILDMILRRVGNDVAAIIIEPCMGN
                     CGAILPKKGFLEFIRAKTSEYGIVFILDEVKTGFRIARGGAQEYYNVIPDMATYAKSI
                     GNGYPIAAFGGKREIMQTIGRGVAQGGTFNNNKPGVAAAFVTLDLMQKLPVHETISKR
                     GQRLMDGIEKIFQVAGIPACINGYPAMFSFAVGLEKVTNQRDWSESDRTYYLRLVEAL
                     VERGVMPDHDPREPWFLCYSHSDADIDETLNAMDDAVRHTLR"
BASE COUNT          669 a          510 c          639 g          669 t
ORIGIN      
        1 gtgggttgca gtaataagat tacactgact acaaccgcag caatcagtat tatcgcgatt
       61 gcgacaatac caatcattgc ctggcgctgg cggcgtagtt cctgaggaga aagtttatct
      121 tcctcagaaa ttgtgttaat ttcctccgat ttgttcccgc tctccatata ctatggattt
      181 taccacaatt gatgctgtat gggatttagg cagagtgccc caagtgacta tctacaaact
      241 tcgaattcac gaccaatttg tttattgata tcaacttcta tgcaaagtcg tccgccagat
      301 ctgtatatct aagtcttagt gcatagattt gcaggagagg atacatatgg caatgcgtca
      361 ctttatcgag atacaagaca ttaccaatga gcgtaatttt catcccttgc cgagtttttt
      421 ggtaaatcta ttgacagtga ttaataaatc tggtaaatta ttttcgtctc tcgttgtcgt
      481 aaatggaaaa caatattaaa atctatgtca aaaatcgaca aaaaagacct gcaaattgtc
      541 aatcttctca tggaagatgg acgcatgtcg gcagccgaga ttgcccgtca ggtgggtaac
      601 atctccgagc gggttgtacg ctatcgtata gatcgaatgg ttaaggaggg gattatttcg
      661 atcaatgcta tcccaaaacc taaggcattg ggatttaccg tcattgcaga tgtgtttatc
      721 gaggttgaga ctggtcggat ccaggaagtg gctaccaagc tgaaagatat ggaatgtgtc
      781 agctatgtgg cgtgcgcaat tggtgagact gatatcagcg tgcagcttgt tgcacatgat
      841 acgggggagg tttatcaatt tgtcacggaa gtgataggga gaaccccagg ggttcgcaaa
      901 accacaactt caatcgtacc gtatacattg aaggatgtat accaatggcg aattccaccc
      961 tcggtgagcg ttgatgatta ggcgggaatt ggttacttgc ggatgatgat ttgattttgg
     1021 agaaataatg gctatccagc gtgaaaaaag caattcgtta caacgcaggg caatggctgt
     1081 tacaccgtta ggtgtcaact cgaacttccg ttattgggga gatggcatta cgccttacgt
     1141 gttaaaggcc aaaggagggc acctgtggga tgtcgatgga aaccggtata ttgattatcg
     1201 cctggcttat ggccccataa ttttaggcca tgcctacgat gaggttgacg cgaaagtaca
     1261 tcaagagatt gatcggggag tcctgttcgc tatgacaagc gagctggaga tcgcagttat
     1321 tgaaaagatt gtggaaatgt gccctggtgt tgagatggcc aggttagcat gctctggcac
     1381 cgaggcaacc atgcatgcta tccgtgtggc gagagcatat acagggcggg aaaagatcat
     1441 caagtttgag gggatgtacc atggctttca agactataca ttgttttcga cttatgcgcc
     1501 ggttgaagcg tatggaaatt accgcagccc aatcccaatt ccggccagct caggaatccc
     1561 ccgggcattg catgatctcg tcataacact tccctttaat gaacccgaaa ttctggacat
     1621 gatcttacga cgagtcggca acgatgtggc tgcaataatc atcgaaccat gcatgggaaa
     1681 ttgcggggcg atattaccga aaaaagggtt tctggagttc atccgcgcca aaaccagtga
     1741 gtacggtatt gtcttcatcc tggatgaggt caagacgggg ttccgcattg cccgaggtgg
     1801 ggcacaagaa tattacaatg tgattcccga tatggctacg tatgcaaagt ctattgggaa
     1861 tggctaccca atcgctgctt ttggtggcaa gcgtgagatt atgcaaacca ttggacgggg
     1921 agttgcccag ggtgggacat tcaacaacaa caaacctggc gtggctgcag cgtttgtcac
     1981 ccttgatttg atgcaaaaac tgccggtgca tgaaaccatc tcaaagcgag gacagcggtt
     2041 aatggacggc attgagaaaa tattccaagt agctggtatt ccagcctgca ttaacgggta
     2101 tccagcgatg ttctctttcg cagtcgggct tgaaaaagtg actaaccaac gagattggag
     2161 tgaaagcgat cggacctact atctacggct tgttgaggcg cttgtagagc gtggtgtaat
     2221 gccagaccac gacccgcgcg aaccgtggtt tttgtgctat tcccatagcg atgcagacat
     2281 agatgaaact ttaaatgcga tggatgacgc ggttcgccat acgcttagat aattattcat
     2341 ccgaactaga gtagccttta catagttgac ggagagattg ggatttgagc gggtttcggt
     2401 cgctcatatc cccccaacta cttctgggtt tttgttagtt ctgaactgat tacggaggtt
     2461 cgtgtgatta ctgaaagaac tactttg
//
LOCUS       DBNHOH010000830         2683 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_244826, whole genome shotgun sequence.
ACCESSION   DBNHOH010000830 DBNHOH010000000
VERSION     DBNHOH010000830.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_244826"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="AC16V7_14970"
     CDS             complement(<1..502)
                     /locus_tag="AC16V7_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR6258472.1"
                     /translation="MSQSPPPPEALPLARVLGIETSCDETAAAVVENGRLILSNLVAS
                     QVELHAQFGGVFPEVASRQHIRTIYAIVEGALQQAHLDLRDVDAIAVTRGPGLPGSLV
                     VGMNVAKGMSLGSGLPLLGINHLEAHIYSAWLYAADAAPVAEPRFPLVVLIVSGGHTE
                     LILMTDH"
     gene            complement(526..1761)
                     /locus_tag="AC16V7_14975"
     CDS             complement(526..1761)
                     /locus_tag="AC16V7_14975"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR6258473.1"
                     /translation="MKSDLDSLMLSNQIDAMLITGPAMHNPPMTYFTGGVHVTGGDLI
                     KKRGEAPVLFYHDMERDEAARTGLATKSLDAYRFPELLKKSNGNWVQATILRYQKMLT
                     DAGLTSGRIALYGKIDAGEAYAIFSGLQGTMPALEFVGQTNDSLLLKARTTKDSDEVQ
                     RIRHMGQITVEVVGLTADFLTSHRAKNGLLVKPDGQPLTIGEVKRRINMWLVERGAEN
                     PYGTIFAIGYDSAVPHSSGTSSDPLRLGETIIFDIYPCETGGGYFYDLTRTWCLGHAT
                     DEAQALYEDVLAVYTQVLSELKPGIHYPRFQQRACELFEARGHPTQHSDPQTQRGYVH
                     SLGHGVGMDIHERPFSGRSAAPDDTLQPGSVVSHEPGLYYPERKLGVRLEDTLWMRTD
                     GSVEILAEYPLDLVLPVKP"
     gene            complement(1776..>2683)
                     /gene="selA"
                     /locus_tag="AC16V7_14980"
     CDS             complement(1776..>2683)
                     /gene="selA"
                     /locus_tag="AC16V7_14980"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HIR6258474.1"
                     /translation="AAVLLALTALARRSAVVIARSQLVEIGGGFRVPDVMKQSGARLL
                     EVGTTNRVHLSDYEAALEERPAMFLHAHRSNFRILGFTSEPALDEIARLAHQASVPLV
                     DDLGSGSLLDTARYGLGHEPTVQESLQGGADLVCFSGDKLLGGPQAGIIAGRADLVSK
                     LKKHPLARAVRADKLCLAALTATLLHYVKDEAERQIPVWRMIAARPEEIKARAQSWIE
                     HLGRGEIIPGESTVGGGSLPGETLPTWLLALDVPSPNRLLGRLRQCQLPIIARTQDDR
                     VVLDPRTVLSEQDSVLLDELGVELA"
BASE COUNT          565 a          781 c          791 g          546 t
ORIGIN      
        1 gatgatcggt catcaagatt agctcggtgt gcccacctga gacgatcagc accaccagcg
       61 ggaagcgcgg ttcagccacg ggcgctgcat cggcagcata caaccaggcg gagtaaatgt
      121 gcgcctccag gtggttaatc ccaagaagtg gaagaccact gcccagcgac atccccttgg
      181 ctacattcat gcccaccaca agggagccag gcagcccagg cccgcgcgtc acggcaatag
      241 cgtctacgtc tcgcaggtca agatgggctt gctgcaacgc accttctacg atggcgtaga
      301 tggtgcgaat atgctggcgg gaggccacct cgggaaagac accaccaaac tgggcgtgca
      361 gttccacctg ggatgcgacc agattggaga ggattaagcg cccgttctca accactgcag
      421 cggcggtttc atcgcaggag gtttcaatgc ccagaacacg tgccaggggt aatgcttcgg
      481 ggggtggtgg agattggctc atgattacct ttccaactaa tttctttatg gcttgaccgg
      541 gaggaccaaa tcgagtggat actcagccag aatctccact gatccatccg tgcgcatcca
      601 cagggtatcc tccaggcgca ctcccagctt gcgctcaggg taatataacc cgggttcgtg
      661 gctgaccacc gacccaggct ggagggtgtc atctggtgca gcgctgcgcc cgctgaacgg
      721 gcgctcatga atgtccatac ccaccccatg tcccaggctg tgcacgtagc ctcgctgagt
      781 ctgcgggtcg ctgtgttggg tggggtggcc gcgggcttca aaaagctcac aggcacgctg
      841 ctgaaagcgc ggataatgga tgccaggctt gagctcgctc aaaacttgcg tatagacagc
      901 gagcacatct tcgtaaagcg cctgcgcttc atcggttgca tgccccaggc accaggtgcg
      961 ggtgagatca taaaaatagc cgcctcccgt ctcacaggga tagatatcaa aaatgatggt
     1021 ctcacccagg cgcaacgggt cgctgcttgt accgctgctg tggggcaccg cggagtcata
     1081 tccgatggca aatatggtgc catatggatt ttcagcgcca cgctcaacca gccacatatt
     1141 tatgcggcgc ttgacctcgc cgatcgtgag cggctgccca tcgggcttga ccagcaggcc
     1201 gtttttcgcc ctgtgggagg tgagaaaatc ggcagtgagc cctaccacct caacagtaat
     1261 ttgtcccata tgccgaatgc gctgcacttc atcggaatct tttgtggtgc gagctttgag
     1321 cagcagggaa tcgttggtct ggccgacaaa ctctagtgct ggcatagtcc cctgcaaacc
     1381 ggagaaaatg gcgtatgcct ccccggcgtc gattttgccg tatagggcga tccgcccaga
     1441 cgtgaggccc gcatcggtga gcatcttctg gtagcgcagg atggtagcct gcacccaatt
     1501 accattgctc ttttttagca gctcggggaa gcggtaggca tccaggcttt ttgtcgccaa
     1561 cccggtgcgt gcagcttcgt cgcgttccat gtcgtggtaa aacaacacag gcgcttcccc
     1621 gcgctttttg atcaagtcac cacctgtaac atgaaccccg ccagtgaaat acgtcattgg
     1681 ggggttgtgc atcgccggcc ctgtgatcag catggcgtcg atctggttgg agagcatcag
     1741 ggagtcaagg tcagatttca ttagagttat tcacctcaag ccaattctac ccctaactcg
     1801 tcaagcaaga cactatcttg ctctgagagc acggttctcg gatccaggac aacgcggtca
     1861 tcctgagtgc gtgcgatgat gggtaactgg cactggcgca ggcgtcctaa taagcggttg
     1921 gggctgggca catccagggc aagtagccag gtaggcagtg tctcgccggg caggctgccc
     1981 ccgcccacgg tagattcgcc tggaatgatc tcgccgcgtc ccagatgctc aatccagctt
     2041 tgggcgcgcg ccttgatctc ttccgggcga gctgcgatca tgcgccagac gggaatctgg
     2101 cgctcagctt catctttcac atagtgtagc aaggtggcgg taagcgccgc caggcaaagc
     2161 ttatcggcgc gcacggcgcg cgctaagggg tgtttcttta gtttggagac cagatcagcg
     2221 cgcccggcga taatcccagc ctgcggaccg cccagcaatt tatcgcctga aaagcacacc
     2281 aggtctgcgc ccccctgcag cgattcctga accgtgggct catgtcccag cccgtagcga
     2341 gccgtgtcca gcagcgagcc agaacccagg tcgtctacca gagggacgct agcttggtgt
     2401 gccaggcgcg cgatctcgtc cagggcgggc tcactggtaa aacccaggat acggaaattt
     2461 gagcggtgag cgtgcaggaa catggcaggg cgctcttcca gggcagcttc ataatcggag
     2521 agatgaactc ggttggtcgt gcccacttct aagagcctgg ctccggactg tttcattacg
     2581 tcgggaacgc ggaagccgcc gccaatctcc accagctgag atcgagcaat caccacggca
     2641 ctccggcgag caagagcggt gagggctaac agcacggctg ctg
//
LOCUS       DBNHOH010000831         2823 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_309499, whole genome shotgun sequence.
ACCESSION   DBNHOH010000831 DBNHOH010000000
VERSION     DBNHOH010000831.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_309499"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..862)
                     /locus_tag="AC16V7_14985"
     CDS             complement(167..862)
                     /locus_tag="AC16V7_14985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258475.1"
                     /translation="MYKLSITTAILVFSLGLAACSQLNPTDTGELIFPTVGPDKPLDT
                     QFPLPGDLPVALQTPTIEALAPVQPTQTVESPVIPQINVERSVSSLETPVYRVQAGSP
                     VAMANFIQPEAGCNFMGVGGQPFDLSGQPVVQLVVEVGGSLAGSDVFHLQLSGNAPVL
                     GPGGFHVVLSDHPIESNGTLWILLYDLAGQPLTEKIYFSTYNDCSRNFTLINFVQVDP
                     SSDYRINFPIIYR"
     gene            993..2453
                     /locus_tag="AC16V7_14990"
     CDS             993..2453
                     /locus_tag="AC16V7_14990"
                     /inference="COORDINATES: protein motif:HMM:NF024919.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HIR6258476.1"
                     /translation="MKHGYWSLACILVLVIITSLTRAWVAKANQSEAQEPEPTPVSHT
                     ITPSNPDPFTSTPQFTATDIFTPTSTLEPTNTIIPATPTVTPITSPTQLPEQLNAESP
                     TESSSPVSIYLPLILRQDAPLPPPNYIPPVKVLICDSLSSPKSIPDNYDLGINDTINI
                     SDQRKIVDIDVRLDISHTWVGDLNVELTHLEANQSVKLINRPGRSGSGLGCGYDNIKA
                     ILDDEISSPVENECVASPAAISGIYIPNQPLGWFDGDNLDGRWRLNVSDRSSASTGSL
                     NGWCILATVSENPEPPIPTPTPPTTPGSAIINGVTGKAQSLPLDCESRSAVDWAKYFG
                     VHIEELDFFDLLPHSDNPDKGFVGSVNGTWGQIPPNPYGVHAEPVAEVLRDHGLEAYA
                     HRPLSWAELKAEIAAGRPVIAWIIGSVNNGIPVYYTPSDGLHTVVARYEHTVIVTGYD
                     SNNVYYLNGATIYSRTISQFLDSWSALGNMAITAHP"
     gene            complement(2437..>2823)
                     /locus_tag="AC16V7_14995"
     CDS             complement(2437..>2823)
                     /locus_tag="AC16V7_14995"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR6258477.1"
                     /translation="MQDPSLLWPCMPFLDIFLCNAYEARRLTGEKEPHHAASALRTRG
                     AKAVVVKLGKDGCWVESTDFNGHIDAPRVEIVDTTGAGDAFAAGLIRGLSAGDGLYDA
                     CVAANQAGARMVGYLGAVGGWIKDEQ"
BASE COUNT          783 a          740 c          610 g          690 t
ORIGIN      
        1 attaaccact cgtagccatt catcgttgcc agcatcaatt aactgcattc aagcctcgta
       61 aaccaagtaa aatcgcacag gcattatatc ccaactaccc gtaagattaa tatacttggg
      121 ttgggtactt ttagagacaa tactaggaca gcaagtcagt atatgtttat cgatatatta
      181 ttgggaaatt gatgcggtaa tcagaagaag gatcaacttg gacaaagttg attaaagtaa
      241 aattgcgtga gcaatcatta taagtggaaa aataaatctt ctctgtcagg ggttgtcctg
      301 caaggtcgta aagtaaaatc cataacgtcc cattcgattc aatgggatga tccgacagaa
      361 caacatgaaa acctccaggg cccagaacag gggcattgcc gcttaattgc agatgaaaaa
      421 catcagagcc agctagagaa ccgccaacct caacaacaag ttgtacgacc ggttgcccgc
      481 tcaaatcaaa tggttgccca ccaacaccca taaaattaca ccccgcctca ggctggataa
      541 agttagccat ggcaacgggt gagccagctt gaactctata aacgggcgtc tcgagtgaac
      601 tcacactcct ctccacgttt atctggggaa ttacaggcga ctccactgtt tgcgttggct
      661 ggactggagc aagcgcttca atcgtagggg tttgcaacgc gaccggcaaa tcacctggca
      721 atgggaactg cgtatccaag ggcttatcag gtcctacggt tgggaagatt agttctccgg
      781 tatcagttgg attaagttga gaacatgcag ccaagccaag ggagaaaaca agaatggcag
      841 tagtaatcga cagcttatac atcacatgct ccttcatcca ctgacaggtg ttacaagttt
      901 catgcccaat atcgaagtac ctcatggtta tggtaagatt ggttccaatt acgagcgcaa
      961 gcacaatgta gcagaaatac attcaaaccc gcatgaaaca tggatattgg tcgcttgcat
     1021 gtatcttagt actggttatt ataaccagcc tgacgcgcgc ttgggtggcg aaagccaatc
     1081 aatcggaggc gcaggagcct gaacccacac ctgtttccca tacgatcaca cccagcaacc
     1141 cagatccttt cacctcaaca ccacaattca cagctacaga tatttttact cccacttcga
     1201 ccctggagcc gacaaacact attatccccg ccacacccac cgtaacccca ataaccagcc
     1261 cgacgcaact tcccgaacaa cttaacgccg aatcgccaac tgaaagctcg tcccctgtat
     1321 cgatttatct tccgcttatc ctccggcaag atgcccctct tccaccgcca aactacatac
     1381 caccggtcaa agttctaatt tgcgatagcc tctcttcgcc caaatcgatt ccagacaatt
     1441 acgacctggg catcaacgac acgatcaaca tcagcgatca acgcaagatt gtcgatatcg
     1501 atgtccggct ggatatatcc catacctggg tgggtgattt aaatgtggaa ctaactcatt
     1561 tagaagccaa tcaatccgta aaattaatta atcgccccgg tcgttctgga tcaggtctag
     1621 gttgcggtta cgataatatc aaagcgattc tcgatgacga aatatcgtct cctgtggaaa
     1681 atgaatgtgt agccagccct gcagcgatat cgggcattta tatccctaac cagccattgg
     1741 gctggtttga tggcgacaat ttagacggtc gatggcgact gaacgtttca gatagatcat
     1801 ctgcaagcac aggcagcttg aatggatggt gtatcctcgc cactgtgagt gaaaatcctg
     1861 agccgccaat ccctacacca acgcctccaa ccacgcctgg gtcagcgata ataaacggtg
     1921 ttactggaaa agctcagtcg ctgccactgg attgcgaatc gcgttctgcc gtcgattggg
     1981 cgaaatattt cggtgttcat atcgaggagt tagatttttt cgatctttta ccccattcag
     2041 acaatcccga taaggggttt gttggatccg tcaatggcac atggggacaa attccgccta
     2101 acccttatgg tgttcatgcc gaaccagtgg cagaagtttt acgcgatcat ggattggagg
     2161 cttatgccca tcgcccattg agttgggctg agctcaaagc agagatagcc gccggcaggc
     2221 cagtgattgc ttggattatc ggatcggtca ataatggcat cccggtatat tacaccccct
     2281 ccgatggtct ccataccgtt gttgcgcgct acgaacacac cgttattgtg accggttacg
     2341 attcaaataa tgtgtattac cttaatggag cgacaatcta ttctcgaacg attagccaat
     2401 tccttgattc gtggtctgcc ctagggaata tggctatcac tgctcatcct tgatccatcc
     2461 ccctaccgca cccaaataac ctaccattcg agctccggct tgattggctg ccacgcaggc
     2521 atcatacaag ccatcaccag ctgataaacc tcggatcaga cctgcagcga acgcatctcc
     2581 agcgccggtg gtatcgacta tttcgactcg tggagcatca atatgcccgt tgaagtctgt
     2641 gctctctacc caacaaccat ccttgccaag ctttacaacc actgctttag cccctcgagt
     2701 tcgtaaagcg cttgctgcat gatgaggttc cttttcacct gttaatcggc gcgcttcata
     2761 tgcattgcaa agaaaaatat ccagaaatgg catacaaggc cagagcaaac tgggatcttg
     2821 cat
//
LOCUS       DBNHOH010000832         2355 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1014986, whole genome shotgun sequence.
ACCESSION   DBNHOH010000832 DBNHOH010000000
VERSION     DBNHOH010000832.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1014986"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..787)
                     /locus_tag="AC16V7_15000"
     CDS             complement(188..787)
                     /locus_tag="AC16V7_15000"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6258478.1"
                     /translation="MVRYKKEEREQFLSRTRQLLLDSAAEEFAHKGYAEANINHISKS
                     AGFAKGTIYNYFASKEELFLDLLVTTAEQHIEEIIAYVNQEDDPRRRLERFFEAGFAF
                     VSQNLARTRVLINTTYGPNPDFKLQLYQAYQSLFQYVSRQIVALGIDEGIFREVEPNS
                     MGTLLMTIYLGTASQVSEDGQIWLDPKQVADFAWHALRK"
     gene            complement(1042..1215)
                     /locus_tag="AC16V7_15005"
     CDS             complement(1042..1215)
                     /locus_tag="AC16V7_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258479.1"
                     /translation="MQKYQGMVNLVLKAVALAMAVASLVMGILGTSTVEAIQTLLSIG
                     LFSLALAALQRTE"
     gene            complement(1334..1753)
                     /locus_tag="AC16V7_15010"
     CDS             complement(1334..1753)
                     /locus_tag="AC16V7_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258480.1"
                     /translation="MVHALEESRRVLTHNGRLIDIRPLSSYPPVEVITSNGARLAGRA
                     DDSLGIPDDQAANNAIADAVRQGIFFKDADERFEFFYYWDTMEQMQAYIKDRWDDWVI
                     LRDTTLQNAAQIISESTGQIQLRITLTMLISVYGKRG"
     gene            complement(1772..2074)
                     /locus_tag="AC16V7_15015"
     CDS             complement(1772..2074)
                     /locus_tag="AC16V7_15015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6258481.1"
                     /translation="MSVYVLRDPEESEIQYLDRLGNLKGMRVLEIGSGDGRMTKRYAA
                     MARSVVGIDPDLESLAIAAKSQPDSSLSDVNLALALAQALPFPDEHFDRVIMAWSL"
     gene            complement(2163..>2355)
                     /locus_tag="AC16V7_15020"
     CDS             complement(2163..>2355)
                     /locus_tag="AC16V7_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258482.1"
                     /translation="ADFEGGNGYRYMSESARSVSQEEMPMEQGHLFKVGDLCPECGQA
                     AVVNEEGCRKCYSCGFSEC"
BASE COUNT          609 a          630 c          492 g          624 t
ORIGIN      
        1 ctcatcgagc tcagaggctg agagttggtt tgtagatatc acccgaacag ttccactggc
       61 ttccagcacg ctcgcaatcg cctctgcgac tttttgggta ttgccaaatt tagaaaaata
      121 tacaatcgtt gatctcatta gtattcctcc aatcaattta cgaaatattg ccagcttcct
      181 ggacgttcta ttttcgcaag gcgtgccaag cgaaatcagc aacctgcttg gggtccagcc
      241 agatctggcc atcttcgcta acctgtgagg ctgtgcccag gtagatggtc atcaacagtg
      301 tacccatgga gtttggttcc acctcccgaa agattccctc atcaatccct aaggcaacga
      361 tttgtctact gacatactga aaaagtgact ggtaagcctg atagagctgc agcttaaaat
      421 ctgggtttgg tccataggtg gtgttgatca ggacccgtgt tcgtgcgaga ttctgcgaca
      481 cgaatgcaaa gcctgcttcg aaaaatcgct ccagccgacg gcgggggtcg tcttcttgat
      541 ttacataagc aatgatttct tcaatgtgct gctcagctgt cgtcactagc aggtccaaga
      601 agagttcttc tttgctagca aagtaattgt atatagtgcc cttggcgaag cccgctgact
      661 tggatatatg gttgatattt gcctccgcat aacccttgtg ggcaaattct tcggctgccg
      721 agtctaataa caattggcgg gtcctgctca aaaactgctc tcgttcttct tttttgtagc
      781 gcaccatcga aacctctctt tatatgggtg gtcagtcacc catattattg cacaaataac
      841 gcggtctgtc aagataatta atcgatttat ccactgtttt gtgtaggaaa cggatcgtaa
      901 atttcacccc taagcgaaaa aaatcccagc tgggccaatg gcgtagccaa ccaggttgct
      961 cttgcacagt aaaaccaggc aagcgttctc gaggcacccc cccactaccc ccactaattc
     1021 tttggcaaca gcgtatccag cctattccgt cctttgaaga gccgccagcg ccaggctgaa
     1081 caaccctatg cttagcaagg tttggatagc ctcaaccgtg cttgtcccta atatacccat
     1141 gacaagactg gctaccgcca tggctagcgc aactgctttc aaaactagat tgaccattcc
     1201 ctgatatttc tgcatatctc ctactccttg tattaggaat tcaaaatgaa attattcaag
     1261 agtatttcaa aaaggttaaa gttgtaaatt gctatcgcat ttaacaaata gcgaacctct
     1321 atccgtttga gatttaaccc cgcttaccgt aaacagagat aagcatggta agagtgattc
     1381 gtaattgtat ttgaccagtg ctttcactga ttatctgggc tgcgttctga agagttgtgt
     1441 ctcgcaagat aacccaatca tcccatctat cctttatgta ggcttgcatt tgctccatag
     1501 tatcccaata ataaaaaaat tcaaaacgtt catcggcatc cttgaagaaa atgccctgtc
     1561 gaacggcatc tgcaatggcg ttattggctg cctgatcgtc aggtatacca agggaatcat
     1621 cggctctacc agccaaccta gccccattgc tggtaatcac ttcaacgggc ggatatgatg
     1681 acagtggacg gatatcaatc aaacgcccgt tatgcgtcaa tactcgccgg ctttcctcaa
     1741 gggcatgaac catgccctca tgcgctactc atcaaagcga ccaggccata atcacgcggt
     1801 caaagtgctc atctggaaac ggcagcgctt gcgctagagc cagcgccagg ttgacatctg
     1861 agagtgaact gtccggctgt gacttcgcgg caattgccag gctttccaga tctggatcga
     1921 tgcccacaac ggatcgtgcc atcgcggcat aacgcttagt cattcggcca tccccagaac
     1981 caatttccag cacacgcatc cccttgagat tgcccagccg atcgagatat tggatttcac
     2041 tctcttcagg atctctcaag acataaacgc tcatatgaac tccaatcaaa tgcttctttt
     2101 caaagtagaa aaaacaggca gggattgttg ctccctgccc actcctccat gattggaaaa
     2161 ttctagcatt cgctaaaccc gcacgaatag cattttctgc atccctcttc attcaccacc
     2221 gctgcctgac cacactcggg acacaaatct cccactttga acaaatgccc ctgctccatg
     2281 ggcatttcct cctgggaaac cgacctggct gactcgctca tatagcggta accgttgcca
     2341 ccttcgaaat cagcg
//
LOCUS       DBNHOH010000833         2190 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1334997, whole genome shotgun sequence.
ACCESSION   DBNHOH010000833 DBNHOH010000000
VERSION     DBNHOH010000833.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1334997"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="AC16V7_15025"
     CDS             complement(<1..579)
                     /locus_tag="AC16V7_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6258483.1"
                     /translation="MPKQTLLELQNLTTVFPTRRGQVRAVESLNLELFEGEILGIVGE
                     SGCGKSTVLLSILRLIRQPGYIASGKIRYRGRDLRELSTPAMRSVRGKEIAMIFQDPQ
                     STLNPAFTVGEQIREALRLHHIVPGNPYPWPLDYVVRTREKKRVLDVMEEVGIPSPMD
                     RYASFPHQFSGGMQQRALIATALVCGPALLLAD"
     gene            complement(603..1541)
                     /locus_tag="AC16V7_15030"
     CDS             complement(603..1541)
                     /locus_tag="AC16V7_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6258484.1"
                     /translation="MEVAPQVRTPFSHQVYVWRIRLAIWRQNTAEFLYELRKHPLALL
                     GGLVIVVYVVLAIFAGQVTVYDPVRGNLRLRLDPPAWEEGGSWEYPLGTDAQGRDLLT
                     RIIYGARVSLLVGFLSVGISVILGMITGTLAGYYRGWFDNLISRFADLLLAFPFLIFA
                     IGMMAFLGPGFINLILALTFKGWVEFFRLVRGEMLSEKTQEYVEAARVAGLSHLAIIA
                     REIIPNIIQSVFVLGTLRIGYMIIMEASLSFLGLGIPPSIPAWGSMVSAGRDYMLVAW
                     WVSTLPGIAILILVLAINLFGEGLRDILDPRLKIEG"
     gene            complement(1600..>2190)
                     /locus_tag="AC16V7_15035"
     CDS             complement(1600..>2190)
                     /locus_tag="AC16V7_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6258485.1"
                     /translation="LGISMPGFWLGIILILFFSVRLNWLPVQGRITFGTNLQEITGYF
                     VLDSLLSGNREALISSLKHLILPSITLAAPVAAVVARVLRSSMLETLRSDYVTLARSK
                     GATEWSVVLKHALRNALIPTVTVVGLQVGILLGGNMVVETVFGWPGLGRMVVSAIFDR
                     DFPLIQGSVMIYAFTFVMANLIVDVLYTYLNPKISL"
BASE COUNT          563 a          594 c          591 g          442 t
ORIGIN      
        1 atctgccagc agcagtgctg gcccgcatac cagcgctgtg gcaatgaggg cgcgctgttg
       61 catcccaccg gagaactggt gagggaaaga tgcgtagcga tccatgggcg aggggatgcc
      121 cacttcctcc atcacgtcca ggacgcgctt tttctcacgc gtgcgaacga cgtaatcaag
      181 aggccaggga tatgggttac ccgggacgat atggtgcagg cgcaaggctt cgcgaatctg
      241 ctcgcccact gtaaaagccg gatttagggt ggactgtgga tcctggaaaa tcatggcgat
      301 ttccttacca cgcacggagc gcatcgctgg tgtcgagagt tctcgcagat cgcgaccccg
      361 gtagcgaatc ttgccggacg caatataacc tggctggcgt ataaggcgca agatggagag
      421 taatactgtg cttttaccac agccagattc gcctacgatc ccgagtatct cgccctcgaa
      481 taattcgaga ttgagactct cgacggcgcg cacttggccg cgccgggtag gaaatactgt
      541 cgtaaggttt tgtagctcaa gtaaagtctg ttttggcatg cgggcctcat acgtgctgtg
      601 gattaacctt caattttcag gcggggatct aagatgtcac gcaatccctc gccgaacaag
      661 ttgatcgcta gtaccaggat caagatggca ataccgggca aggtggaaac ccaccacgct
      721 actagcatat aatctcgccc tgctgagacc atcgaacccc aagccgggat ggagggaggg
      781 atgccaagcc ctaaaaagct cagactggct tccatgatga tcatatagcc tattctcaaa
      841 gtgcccagca caaacaccga ctggataata ttggggataa tctcgcgagc gataatcgcc
      901 aggtggctca agccagccac acgggcagct tctacgtatt cttgggtctt ttcagagagc
      961 atctctccac gcacaagccg gaagaactcc acccaccctt tgaaggtcag tgccaggatc
     1021 aggttgatga aacccggccc caagaaagcc atcataccaa tagcaaagat caaaaatggg
     1081 aatgctagca ataggtcggc aaagcgcgag atcaggttat cgaaccagcc tcggtaatag
     1141 ccagccaggg tgccggtgat catgcccaaa atcactgaaa tgcccactga tagaaaacct
     1201 acaagtaagg aaacccgagc gccatagatg atgcgcgtga gcaaatctcg gccttgggcg
     1261 tcggtgccca gcggatactc ccagctgccg ccttcctccc aggccggggg atccaggcgc
     1321 aagcgtagat tcccccggac cgggtcgtaa acggttactt gcccggcaaa gatggctaat
     1381 accacgtata caacgatcac caaaccaccg agcaatgcca gagggtgctt gcgcagctcg
     1441 tatagaaatt cagcagtatt ttgtcgccaa atcgccagcc ggattcgcca cacatagacc
     1501 tggtgcgaga atggggttcg aacttggggg gctacttcca tcggtttagc tttcctatcg
     1561 atttaatgtg tgccattgaa atgaatacaa gttaggtgtc tataaggaaa tctttggatt
     1621 gagataggta tagagtacat ccacgatcag gttagccatc acgaaggtga aagcatatat
     1681 catgacggaa ccctggataa gcggaaaatc gcggtcaaag atggcgctca ccaccatgcg
     1741 ccccagtccc ggccaaccaa agacggtttc cacaaccata tttccgccca gcaagatgcc
     1801 cacttgcaag ccgactaccg tcacagtggg tatcagggcg tttcgcaggg catgctttag
     1861 cactacgctc cattctgtgg cgcctttgct acgcgccaga gtgacgtaat ccgagcgcaa
     1921 cgtctccaac atgctggagc gcagcacgcg ggctactacc gccgcaactg gcgctgcgag
     1981 ggtaatggaa gggagtatca agtgcttcaa gctgctgatc agcgcctccc gattgcctga
     2041 gagcaggctg tccagtacaa aatagcctgt gatttcctgc aggttggtac cgaaggtaat
     2101 cctgccctgc actggcagcc agttcaggcg caccgaaaag aagaggatga ggatgatgcc
     2161 taaccagaaa cctggcatcg agatgcccaa
//
LOCUS       DBNHOH010000834         2773 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_245281, whole genome shotgun sequence.
ACCESSION   DBNHOH010000834 DBNHOH010000000
VERSION     DBNHOH010000834.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_245281"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="AC16V7_15040"
     CDS             <1..775
                     /locus_tag="AC16V7_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258486.1"
                     /translation="APILVYQYIVMNSDPLFSEWMIQNITPSPPIWDAMISLSPLLLL
                     VPYGAWVIILNRNKEQRLLLVWVVVSLILLYTPFSLQRRFMMGIYVPVAILAAMSIDW
                     MIKDGRRFWFRSMLLLLIVLPTNLLILRAAWHGIQTRDDRLYLSKDEIDALAWVEENS
                     PSDALILAAPETGLFIPAITGRRVIYGHPFETVHAEKQEERVLNFYQGVALPNPKAWL
                     GLVDYVYYGPREREIGKNELLDALKAVFHNQSVVIYATK"
     gene            787..>2773
                     /locus_tag="AC16V7_15045"
     CDS             787..>2773
                     /locus_tag="AC16V7_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258487.1"
                     /translation="MGKHWKKPLRDSRVVGFLLLLPFLVILPNLDTFPYPRYTGAYSD
                     ITVSHYPNAYFLRQELLAHRPIPLWSPTILSGYPFIANPLSGLLYPPGWLGLLLPIPA
                     GFHVVIALHLVLGGVGMLRLLRAEGMSYPAALFGGMAFELMPKIAAHYGAGHLTLLYA
                     LAWTPWLLLTWKEEEKQRVDKGNESKRWIKPSLVLTMIILADVRWAAFATLLLWAYAI
                     AHSQHFLVAVRGLTRQTGLAVLLSAPLSIPLIEFIILSSRTTMTAADRLTLSLSPARI
                     LGFIFPDFGGFHEWALYPGAVTLCLALVAIAGIRHKSGLAFWIWVAVASTLVALGSYI
                     PGMQYLARLPGFSLLRVPARALFITGLAIAALAGYGLEALIGQLRGLNRRVAKLVLVG
                     LVSFALVLAAGVKFITLNISLSFFWGAGITLLSVLWIMWCINGVAKRFENFWFVGLFA
                     IAIIDWTAVDRSYVLFRPFGEVQTENATSAEWLASQPGLFRVYSPSYSIPQATAIHAG
                     LQLADGVDPLQLENYVRFMEQATGVPRVGYSVTMPPFADGDPASANSGYVPDASRLGM
                     LNVRYVASAFDLTVDGLEFKTEIAGTRLYENMRVLPRAWIQPANALPGEQASPATVYD
                     WQPNRITAETQGPGLFVISEVHYPGWNAYVDGERVPIE"
BASE COUNT          628 a          603 c          718 g          824 t
ORIGIN      
        1 cgcgcccatt ctggtttacc aatatatcgt catgaattcg gatcccttgt tttctgaatg
       61 gatgatccaa aatattacgc catccccacc aatttgggat gccatgatct cactttcgcc
      121 gttgttgtta ttggttccat atggggcttg ggtgataatt cttaacagga acaaggaaca
      181 gcgattattg cttgtgtggg tcgttgtaag cttaatcttg ctgtacacac cttttagttt
      241 gcaacgacgc ttcatgatgg gcatctacgt ccctgtagcc atcctagcag ctatgagcat
      301 tgattggatg atcaaggatg ggcgtcgatt ttggtttcgc agtatgctac tgttgctcat
      361 agtattgcca acgaatttac tcatcttgcg ggcagcctgg catggaattc aaacccgcga
      421 cgatcgtcta tatcttagta aggatgaaat agatgcgttg gcatgggttg aagaaaattc
      481 accctctgat gccctcattc tggcggctcc agaaacgggt ttatttatac ctgcaataac
      541 tggccgacgt gttatttacg gtcatccatt tgagacagtt cacgctgaaa agcaggaaga
      601 acgagtgtta aatttctacc agggtgtagc tttgccaaat ccgaaggctt ggttaggctt
      661 ggtggattat gtatattatg gaccacgtga aagagaaata ggcaaaaatg aattactcga
      721 tgcgttgaaa gcagtattcc ataatcagag tgtggtcatt tatgcgacaa aatgaatggt
      781 ggtgtgttgg gtaagcactg gaaaaaacct ttgcgggata gccgggtggt cggcttcttg
      841 ttgctgctgc catttcttgt catccttcca aacttagata cttttccata tccacgttac
      901 actggggctt attcggatat tacggtcagc cattatccca acgcgtactt cttgcggcag
      961 gaattattgg ctcaccgccc gatcccatta tggtcaccga cgatcctgag tggctaccca
     1021 tttattgcta atccactatc gggtctgtta tatccaccag gttggcttgg gctgctattg
     1081 ccaataccgg ctggattcca tgttgtgatc gctctgcatc ttgtgttggg tggtgttggg
     1141 atgctgcgct tgttgcgcgc agaaggcatg agttatcctg cagctctttt tggggggatg
     1201 gcattcgagt taatgccaaa gatcgcagcc cattatggag cgggccactt gacactcctg
     1261 tatgcacttg cctggacacc gtggctatta ttaacctgga aagaggagga aaagcaaaga
     1321 gttgataagg ggaatgagtc caagcgctgg ataaaaccga gtctggttct aacgatgata
     1381 attctggcag atgtacgctg ggctgcattt gctaccctgt tgttatgggc atacgctatt
     1441 gcgcatagcc aacatttcct ggttgctgtt agaggtttga ctcgacaaac tggattggct
     1501 gtcttgcttt ctgctccatt gtcgatacct ctaattgaat ttattatcct ctcgtccagg
     1561 accactatga cggcggcaga tcgcttaaca ttgtcattgt cacccgcacg catattaggt
     1621 tttatatttc cggattttgg cggttttcat gagtgggcgc tttatcctgg agctgtgact
     1681 ctttgtcttg cactggtggc aattgcaggt atcagacata aatctggtct agcattttgg
     1741 atttgggtag cggtggcaag tacactggtg gcgttaggta gctatatacc agggatgcaa
     1801 tacctggctc gtctacccgg ctttagcctg ttacgcgttc cagcgcgtgc cttattcata
     1861 acgggacttg ccatagctgc attggcagga tatggactcg aggcactaat tggccaattg
     1921 agagggctga ataggcgagt tgcaaagctc gtcctggttg ggctggtgag ctttgcgctg
     1981 gttttggcgg ccggagttaa attcatcacc cttaatatct cgctttcatt tttttggggt
     2041 gcggggataa ctttgctttc tgtactctgg attatgtggt gtatcaatgg ggtagcaaaa
     2101 cgttttgaaa acttctggtt tgtggggttg tttgccatcg ccataatcga ttggacagct
     2161 gttgaccgtt cttacgtttt atttcgccca tttggagagg tccagactga gaatgcaact
     2221 tccgccgaat ggttagctag ccaacctggg ttgtttcgag tgtactcacc ctcctatagt
     2281 attccccaag caacagccat tcatgcaggc ttgcagcttg cagatggggt agacccactt
     2341 cagctagaaa attacgtaag atttatggaa caggctactg gtgttccacg tgtagggtat
     2401 agtgtgacca tgccgccatt tgccgatggt gacccggctt cggctaattc aggctacgta
     2461 ccagatgcat ctaggttggg aatgctcaat gtgcgatatg ttgcttcggc atttgacttg
     2521 actgtagatg ggttggagtt caagaccgaa attgccggca cgcgccttta tgagaacatg
     2581 agagtgctac cccgagcgtg gattcaaccc gctaacgcat tgcctggcga gcaagcaagc
     2641 ccggcaacgg tttatgactg gcaacctaat cggataactg ccgaaacgca aggtcctggc
     2701 ctgtttgtta tctctgaagt acattatcct ggttggaatg catatgtaga tggcgaaagg
     2761 gtacctatcg agc
//
LOCUS       DBNHOH010000835         2120 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1079237, whole genome shotgun sequence.
ACCESSION   DBNHOH010000835 DBNHOH010000000
VERSION     DBNHOH010000835.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1079237"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..845
                     /locus_tag="AC16V7_15050"
     CDS             3..845
                     /locus_tag="AC16V7_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6258488.1"
                     /translation="MTGILIGGIFALISVGLSLIWGVMRIINFAHGEFLMIGMYISYL
                     LTAKAGWDPYLTVLVTTPLLFLVGVVIFQVSIRPILDHPSMNQIMLTLGLSLILQNLA
                     LVIFKADVLVTSTTYTRMTFTVGPVVVRVAQLIAFLGSGLAAILLYWFLQKTDIGRAI
                     RAAAQNRDAAVLVGINVQRTYLLAFGIGSACLGVAASFMIPFYYTSPTVGLFFGLIAY
                     VVVVLGGLGNFMGALVAGLIIGLTESVGASLLPGSLARVLTFGIFVLVLLFRPQGIFG
                     GKQR"
     gene            842..1840
                     /locus_tag="AC16V7_15055"
     CDS             842..1840
                     /locus_tag="AC16V7_15055"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6258489.1"
                     /translation="MNALRNSSAYLILIAVILLIVPLFFTEYALHIAVLVFLYAYLAT
                     SWNILGGYAGQHSLGHSLFLGIGAYTSTFLFTKLGVTPWLGMWVGAAFAGLVGWFVGY
                     LCFRYGLKGPYFALVTIALAEAAVYLGNNIEFIGGAQGLEVKWEGHQPALMQFEGKAG
                     YYYIILIMTGLAIWLTHWYSRQRFGYRLVAVRENEDAAEALGVNTLRTKVQASILSAC
                     LTALGGTFFAQYFTYISPRNVFGEAPSIQILLFAIIGGLGTVWGPAVGALVLVPIAEL
                     ARSWLGGSFAGAHLLLYGTVMVIVMLFMPKGIMGLVQNSRARLANRRLPTTESEAA"
     gene            1837..>2120
                     /locus_tag="AC16V7_15060"
     CDS             1837..>2120
                     /locus_tag="AC16V7_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6258490.1"
                     /translation="MSMLVVSDLSRHFGGLTAVQNLSFSIEPGEIVGLIGPNGAGKTT
                     TFNLLTGFTPPSSGEITFSGKLLNGLKPHDICQLGLTRTFQIVQPFPDMTV"
BASE COUNT          427 a          542 c          556 g          595 t
ORIGIN      
        1 tgctgacagg aatattaatc ggcggaattt ttgcattaat atcggttggt ttgagcttga
       61 tttggggtgt aatgcgcatt atcaacttcg cacatggcga gtttttaatg atcggcatgt
      121 atatctcgta tctattgaca gccaaagcag ggtgggatcc ctacttgacc gtcctggtca
      181 ccacaccctt gctttttctc gtgggagtcg tgattttcca ggtgagcatt cgccccattc
      241 ttgaccatcc ctcaatgaat cagatcatgt tgacattggg gctgtcgcta atcttgcaaa
      301 atctggcgct ggtcatcttc aaggccgatg tgctggttac atcaactacc tacacgcgca
      361 tgaccttcac tgttgggcca gtggtagtcc gggttgccca gctgattgcc tttctgggct
      421 caggtctggc agccattttg ctttactggt tcctacaaaa gacagatatc ggccgcgcaa
      481 ttcgggcagc cgcccagaac agagatgccg cggttctggt gggcattaac gtgcagcgca
      541 cttacctgct agctttcgga atcggttctg cgtgcctggg tgtcgccgcg tcgtttatga
      601 tcccgtttta ttacacctcg ccgacggttg gactcttttt cggtctgatc gcttatgtgg
      661 ttgttgtgtt gggcgggcta ggcaacttta tgggcgcttt agtcgccgga ttaatcatcg
      721 gcctgaccga atccgtggga gcttcactgc tccctggctc attagcgcgc gtactcacgt
      781 ttggtatttt cgtccttgtg cttctattcc gcccacaggg tatttttgga ggtaaacagc
      841 gatgaatgct ctgcgtaact cgtcagctta tttgatcctc atcgcagtaa tcttgctcat
      901 tgtgccacta tttttcacgg agtatgcact gcatatcgcg gtactggtgt ttctctacgc
      961 ttacctagcc acttcatgga atatactggg tggatacgcc gggcaacact ctcttggaca
     1021 cagcctattc ttagggattg gggcttacac ctccaccttt ctgttcacca aattgggcgt
     1081 cactccatgg ctaggcatgt gggtgggcgc agccttcgca ggcctcgtag gttggttcgt
     1141 cggatattta tgcttccgct atggtctgaa aggtccgtac tttgccctag tgaccatcgc
     1201 tttggctgaa gcagcagtat acctgggcaa caacatcgag tttatcgggg gcgcacaagg
     1261 attagaggtg aaatgggaag gtcaccaacc tgcattgatg caattcgagg gtaaagcagg
     1321 ctattactat attatcctga ttatgaccgg gctggcgatc tggttgacac attggtacag
     1381 ccgtcaacgt tttggctacc gattggtcgc cgtgcgcgaa aatgaagatg ctgccgaggc
     1441 attgggagtg aataccctgc gcacaaaagt acaggcttcc attttaagcg cttgcttgac
     1501 tgccttaggt gggacatttt tcgctcaata ctttacgtat atcagtccgc gaaatgtctt
     1561 cggagaggct ccctcaatcc aaatattgct ctttgccatt atcggcggac tgggtaccgt
     1621 gtggggtccg gcggttggcg cgctggtgct ggtgcccatc gccgagctag cgcgctcctg
     1681 gttgggcggc agttttgccg gcgcacatct tctcctttat ggtacggtca tggtgattgt
     1741 tatgctgttt atgccaaaag gcatcatggg actggtgcaa aatagccggg cgcgccttgc
     1801 aaacaggcgc ttgccaacaa cagaaagcga ggcagcatga gcatgctggt tgtctccgat
     1861 ctgagccgtc actttggcgg cctcactgca gtccaaaacc tgtcattctc catcgagcct
     1921 ggagaaattg tagggctgat cggcccaaat ggtgcaggga aaacgaccac atttaacctc
     1981 ctgaccggtt tcaccccacc cagctccggc gaaatcactt tttctggcaa actacttaat
     2041 ggtctcaaac cccatgatat ctgccagctc ggcctgacgc gcacattcca gatcgtgcag
     2101 ccatttccag atatgactgt
//
LOCUS       DBNHOH010000836         6395 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_180391, whole genome shotgun sequence.
ACCESSION   DBNHOH010000836 DBNHOH010000000
VERSION     DBNHOH010000836.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6395)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6395)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6395
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_180391"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1934)
                     /locus_tag="AC16V7_15065"
     CDS             complement(<1..1934)
                     /locus_tag="AC16V7_15065"
                     /inference="COORDINATES: protein motif:HMM:NF013279.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR6258491.1"
                     /translation="MSTNTWRDQGIRDKGCPLIPYLLSLPPSTLLSLQNPPSTNRTER
                     GLIVLAVVFVLLFSASLTLAPAARARSWQVDYPWEHWLGVLIWAGCIIAAHLEINRRL
                     PERDPLLLPLAALLSGWGLMTIWRLVPAVGLRQSLWLLVSGAVFTLGLRLPRDLGFMR
                     RYKYVWLTGGLLLTALTLILGTNPLGYGPRLWLGCCGLYFQPSEPLKLLLITYLAAYL
                     AEMDPFNLPEKQRFMLPWLAPTLLMAGLALSLLLVQRDLGTSSIFIYLYVAVVFIATG
                     NRRIVLYGGLTLLVAGITGYALFDVVRLRVDAWINPWLDPSGRSYQIVQSLLAVANGG
                     LFGRGPGLGYPTLVPISHSDFIFAAIAEENGLMGALVLLLLLALFALRGLRIALRAPD
                     TFRRYLAAGLTAHLVGQSILIIGGNMRLLPLTGVTLPFISYGGSSLLTSFISLLFLEL
                     IGSGEEDHEPAPLPQARPYRWMGAFLLVGLGTLALAGGWWAFIRSPALLARNDNARRF
                     IADRYVQRGALLDRRNTPLVATYGEPGTYSRSVVYPLLSSVIGYSNPIYGQSGLEASL
                     NDYLRGLRGNPGLTTWWNYLLYGQHPPGLDVRLSLDLHIQQAADENLGEHIGAIVLLN
                     SLSGEILAMATHPTFDANVL"
     gene            complement(1894..2775)
                     /gene="mtnP"
                     /locus_tag="AC16V7_15070"
     CDS             complement(1894..2775)
                     /gene="mtnP"
                     /locus_tag="AC16V7_15070"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0033353 - L-methionine cycle [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HIR6258492.1"
                     /translation="MNPPTLAVIGGSGLYAMPGLKDTQELDLTTPYGKTSAPIILGTL
                     EGQRIAFLARHGIGHHISPSEVPYRANIYALKTLGVERIISISACGSLRDDYAPGEIV
                     VPDQVFDFTRGRARSFFGEGLVAHISVADPICPDFSQQVYEAVVAAGGKVHQGGAFIT
                     IEGPRFSTRAESHTYRSWGMSIIGMTTSPEVFLAREAEICYAVMAHVTDYDVWHTSEE
                     PVTVEMVIETLHRNTQLAQEAVRILARELKPERSCSCPDALASALITQPDKIPAETRQ
                     KLAIFVDKYLERSGDKG"
     gene            complement(2772..3203)
                     /locus_tag="AC16V7_15075"
     CDS             complement(2772..3203)
                     /locus_tag="AC16V7_15075"
                     /inference="COORDINATES: protein motif:HMM:NF028007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR6258493.1"
                     /translation="MSATILLILRVLLAASLYAFIGLALYLIWRDLQQQTLPSASRLP
                     PTLTLVHITDEEPVPLSFTTQEIVIGRDPSCDFQLEDSTVSAQHSRLTFRRGHWWIED
                     LRSTNGTSLNQEPVTTPMVIASGDELRCGQITLMVTIGESS"
     gene            complement(3200..3958)
                     /locus_tag="AC16V7_15080"
     CDS             complement(3200..3958)
                     /locus_tag="AC16V7_15080"
                     /inference="COORDINATES: protein motif:HMM:NF023818.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FhaA domain-containing protein"
                     /protein_id="HIR6258494.1"
                     /translation="MGLLKSALAQLEIRLQTLIEGSAARIFTSDDLQHELAGRLIEAM
                     QAGVKQQAGGEMIAPNLYTLTVHPSQVEVVLRDSTLLEQLAQTLQESAGETGLNFLSP
                     PVFRIEEGEEVPPRQFRVVAQISIENLAETSDMIVDAAPDAPLVPVNAFLIVNGVQIF
                     PLAQTTINIGRRRDNQLVIDDKRISRVHAQLRAIKGRYVIFDLDSKGGTFVNDQRIHQ
                     SILYPGDVISLAGVPLVFGQDESRLGETQKLTLE"
     gene            4240..4653
                     /locus_tag="AC16V7_15085"
     CDS             4240..4653
                     /locus_tag="AC16V7_15085"
                     /inference="COORDINATES: protein motif:HMM:NF014058.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0072669 - tRNA-splicing ligase complex
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HIR6258495.1"
                     /translation="MPTPSAGFVERPHTADWELAVWAPDLPTLFEQAARGMYMLAGLR
                     LQEVPHLKRRLELQAIDDESLLVQFLDELLYLQGVEGIAFEKFQLQITGNELKAELDG
                     APVASLDKEIKAVTYHNMKIQAGPRGLEVRIVFDV"
     gene            4769..6220
                     /locus_tag="AC16V7_15090"
     CDS             4769..6220
                     /locus_tag="AC16V7_15090"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017720266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HIR6258496.1"
                     /translation="MVNLSDLKKINDYEWEIPKSFRSDMRVPVRIFATRRLLEAVMDD
                     RSLEQAVNSATLPGLVGHVVVMPDMHQGYGFPIGGVAATRYPEGVISPGGIGYDINCG
                     VRLLASQIEYDAVADQMSALASALNNYCPSGVGTKGHVRVSEAELDEVCRDGSRWAQK
                     KGFATEMDVRRTEENGRLEGADPNKVSKRAKERGRAQIGTLGAGNHFIEVDLVEKVFD
                     AQAAQAMGLTEGYLAVQIHCGSRGFGHQICTDYVNEFQRAVHRYGIKLADRELVCAPL
                     NSPEGEAYLAAMRCAANYAFANRQVLAFYARKAFEEVLAGRVKNWHLHQVYDIAHNMG
                     KIETHEIDGERIKVCVHRKGATRAFGPGFEGLPGEFRAIGQPVLVPGSMGTASWVLVG
                     TQGSMERSFGSTCHGAGRVMSRAQAKKKVWGEDLRNQLESGGIHVRAGSMAGLAEEAP
                     QAYKDVDDVVETVSGAGIARKVARLVPVAVVKG"
BASE COUNT         1479 a         1588 c         1939 g         1389 t
ORIGIN      
        1 tctagcacat ttgcgtcaaa ggttggatga gttgccatcg ccaggatttc gccactcagg
       61 ctattcagca ggacaatcgc gccaatatgc tcgcccaaat tttcgtcggc agcttgctgg
      121 atgtggagat ccaggctcag gcgtacgtcg agcccaggcg gatgttgccc atagagcaga
      181 tagttccacc aggtggtaag tccagggttg ccgcgcagac cgcgcaggta atcattgagg
      241 ctggcttcta accccgattg cccgtagatc ggattcgagt agcctatcac agagctgaga
      301 agtgggtata ccaccgagcg agaatacgtg cctggctccc catatgtcgc taccagcggg
      361 gtattgcggc ggtctagaag agcaccgcgt tgcacatagc gatcggcgat gaagcggcgc
      421 gcgttatcat tgcgggcaag caaagcgggg ctacggataa aagcccacca accgcctgca
      481 agagccaggg tccccaatcc aaccaataga aaggctccca tccagcgata tgggcgcgcc
      541 tggggaagcg gtgcaggctc atgatcttcc tccccactgc cgatcagctc cagaaagagc
      601 agtgaaataa aagacgttaa cagcgaagat ccgccgtagg atatgaacgg gagtgtgact
      661 ccggtcaacg gcaataaacg catgttaccg ccgataatca atatgctttg ccccactagg
      721 tgagcggtta atccagccgc cagataacgc cgaaaagtat ctggagctct caatgcaatg
      781 cgcagaccgc gcagggcgaa taatgccagc aggagtaaca agaccagcgc tcccatgagg
      841 ccgttttcct cggcaattgc ggcgaagatg aagtccgaat gcgatatggg taccagggtg
      901 gggtatccca agcccggacc gcgcccgaat aaaccaccgt ttgctaccgc cagcaaagat
      961 tgaacaattt gatatgaccg cccgcttggg tcgagccagg ggttaatcca ggcgtcaacg
     1021 cgcaggcgca ccacatcgaa tagggcgtaa cccgttatgc cggccaccag cagcgtgagg
     1081 ccgccataca acactatgcg gcggttaccc gtggcgatga ataccactgc gacgtagagg
     1141 tagataaata ttgatgatgt tcccaaatcg cgctggacta aaagtaggga taacgctaat
     1201 cctgccatca gcagagtggg cgccagccag ggcagcatga aacgctgctt ttcgggcaga
     1261 ttgaacggat ccatctctgc caggtaggct gccaggtagg tgatcagcag cagtttgagc
     1321 ggctcagagg gttgaaagta tagcccgcag cagcccaacc ataaacgcgg gccgtatccc
     1381 agcggatttg tgcccaggat gagagtcagg gctgtaagca gcagcccacc ggttagccaa
     1441 acgtatttat atcgccgcat gaatcccagg tctctgggca ggcgcagtcc aagcgtaaat
     1501 accgcgccgg ataccagtaa ccacaagctc tggcgcagac caactgcggg gactagtcgc
     1561 cagattgtca tcaatcccca tccgctcaat aatgcagcta gtggcagaag aagggggtcg
     1621 cgttctggca gcctgcggtt gatctccagg tgtgccgcta tgatgcaacc tgcccaaatg
     1681 agcacaccca gccagtgctc ccaggggtaa tccacctgcc aggagcgcgc ccgagccgcg
     1741 ggagctaggg tcagcgaggc gctaaagagc agcacaaaca ctacagccag gacaatcagg
     1801 cctctttcgg tacggttggt tgagggggga ttttgaaggg atagcaaggt tgagggggga
     1861 agagagagga ggtaggggat aagggggcaa cccttatccc ttatcccctg atctctccag
     1921 gtatttgtcg acaaagatgg ccagcttctg gcgtgtctcg gcaggaatct tgtctggctg
     1981 ggtaattaac gcagacgcca gggcatctgg gcagctgcat gagcgctcag gcttcagctc
     2041 gcgagccaga atacgcacag cctcctgggc aagctgcgtg ttgcggtgca gggtttcaat
     2101 gaccatctcc acggtaacag gttcctcgct ggtgtgccag acatcgtagt ctgtgacatg
     2161 cgccatcact gcatagcaga tttcagcttc gcgggcgaga aagacctcag gtgaggtggt
     2221 catgccaatg attgacatac cccaagagcg gtaggtatgc gattcggccc tggttgagaa
     2281 tcgcggtcct tcgatggtga taaatgcccc gccctggtgt accttacccc cagcagctac
     2341 gacggcctca tatacctgtt gggagaaatc tgggcagatt gggtcagcga cgctgatgtg
     2401 cgccaccaaa ccctccccaa agaaactgcg cgctctgccg cgggtgaaat caaacacctg
     2461 atcgggcacc acgatctcac ccggagcgta atcatcgcgt agagagccgc aggcgctgat
     2521 gctgataatg cgctcaaccc ccagggtttt cagagcgtag atattggctc gatacgggac
     2581 ttctgagggc gagatatgat gtccaatccc atgtcgcgcc agaaaggcga tccgctgacc
     2641 ttccagggtt cccaagatga tcggagcgct ggttttaccg taaggcgtgg tcagatctaa
     2701 ctcttgtgtg tctttcaggc cgggcatcgc atacaggccc gacccgccga taactgccag
     2761 ggtgggtggg ttcatgagga ctctccaatc gtgaccatga gtgttatttg tccacagcgt
     2821 agctcatcgc cgctggcgat caccatgggt gtggtgacgg gctcttgatt caggcttgtc
     2881 ccatttgtgg agcgtaggtc ttcaatccac caatgaccac ggcgaaaggt tagacggctg
     2941 tgctgcgcgg acacagtgct gtcttctagt tgaaaatcac aggatggatc gcgaccgata
     3001 acgatttctt gggtcgtgaa gctcagaggg acaggctcct catcggtgat atgtactaac
     3061 gtgagggttg gaggcaggcg tgatgcgctg ggtaaggtct gttgttgcag atctcgccag
     3121 atgagataga gcgccagacc gataaaggcg tataagctgg cggctaaaag tacgcgtaag
     3181 atgaggagga ttgtggcgct cattcaagcg tgagtttttg cgtttctccg agccgcgact
     3241 catcttgtcc gaagacaagt ggcacgccag ctagggaaat aacatctcct ggatacagga
     3301 tgctctggtg gatacgctga tcgttcacga aagtgccacc cttggagtct aggtcgaaaa
     3361 taacgtaacg gccttttatg gcgcgcagtt gcgcgtggac gcgggagatg cgcttgtcat
     3421 cgatgacaag ctggttatcc ctgcgccgcc caatattaat ggtcgtctga gccagtggga
     3481 agatttggac tccattgaca atcaggaagg cgttcactgg cactagtgga gcgtcgggag
     3541 cagcgtcaac gatcatgtca ctggtctcag ccagattctc aatgctgatc tgggctacca
     3601 cccgaaattg ccggggggga acttcttcgc cttcttcgat tcggaacaca ggagggctga
     3661 ggaaatttag cccagtctct ccagcggatt cctgcaaagt ctgggctagc tgctcaagca
     3721 aggtagaatc cctaaggacg acttcaactt gggagggatg gactgtcagc gtgtacaaat
     3781 tcggcgcgat catctcaccg cccgcctgct gtttgactcc ggcttgcatg gcttcaatca
     3841 gacggccagc cagttcgtgc tgaagatcat cagaggtaaa aattcgcgct gcgctccctt
     3901 caatcaatgt ctgcaaacgg atctcgagtt gagccagcgc gcttttcagc agtcccataa
     3961 ctaaaaaacc tggcgcaatt atagcagggt ttatagataa gggatacttg cccaaccctg
     4021 agtgtttcat aatgggggcg tcatggggtt agcagtaggg caatcgcatt tgggcgattt
     4081 cccaccactg aggagagaaa atggggtgtt ttggtgggag tatgccccca ccaaaacacc
     4141 ccaaacactc tttttacggg attgcgcatg cccctcaggg catatgcaat tgccctgggg
     4201 tgagcagcta tgcttctgtg atttaggtat aataattcta tgccgacacc ttcagccggt
     4261 tttgttgaga ggccccacac tgctgactgg gaattggcag tctgggcacc cgacttgcct
     4321 accttgtttg aacaggcggc gcgcggcatg tacatgctgg caggcctgcg cttgcaggaa
     4381 gtgccgcatc tcaagcgtcg gctcgaattg caggctattg acgatgaaag tctcttggtg
     4441 caatttctag atgagttgct ttatcttcaa ggcgtggaag ggattgcgtt tgaaaaattt
     4501 cagctacaga taacaggcaa tgaattgaag gcagagctgg atggtgcgcc ggtggcatcc
     4561 ctggataaag agattaaagc ggtgacgtac cataacatga aaatccaggc tggaccgcgc
     4621 gggctggaag tgaggattgt ctttgacgtt tgacggcaag cgatctggat gggatcggtg
     4681 tacaagagct gagaaggggg ctttgcgaga ttcgtttgaa attttttgat gtggtcattt
     4741 gcccattacc tgggcaagag gtgtagtgat ggttaacttg agtgatctaa agaaaatcaa
     4801 tgattatgaa tgggaaatcc cgaaatcttt tcgcagcgat atgcgtgttc cagtgcgcat
     4861 ctttgctacg cggcgcttgt tagaagcggt aatggatgat cggtcgctag agcaagcggt
     4921 aaactctgct acgctaccgg gcttggtggg acatgttgtg gtgatgccgg atatgcacca
     4981 gggctatggt ttcccaattg gtggcgtggc ggctacgcgt tacccagaag gggtgatctc
     5041 acctggtggg attggttatg atattaactg cggcgtgcgc ttattggcgt ctcagatcga
     5101 atatgatgct gtcgctgacc agatgagtgc gttagcctct gctttgaaca attattgtcc
     5161 tagcggggta ggtacgaaag ggcacgtgcg cgtttcggaa gctgagttgg acgaggtatg
     5221 ccgagatggc agtcgctggg cgcagaagaa gggttttgct accgagatgg atgtgcgccg
     5281 cactgaggaa aatggccgcc tggaaggtgc tgacccaaac aaagtgagca agcgcgccaa
     5341 agagcggggc cgggcccaga tcggcacact tggggcaggc aaccatttta tcgaggtgga
     5401 tctggtggag aaagtgtttg acgcccaggc tgctcaggcc atgggattga ccgagggtta
     5461 tctagctgtg cagattcact gcggctcgcg cggctttggt caccagattt gcaccgatta
     5521 tgtcaacgag tttcagagag cggtgcatcg ttatgggatt aaactggctg atcgcgagct
     5581 ggtatgcgca ccgctgaact cgcctgaggg agaggcttac ctggcagcca tgcgctgtgc
     5641 agccaactac gcgtttgcta accggcaggt gctggcattc tatgccagga aagcgtttga
     5701 ggaggtcctg gcgggtagag tgaaaaactg gcacttgcac caggtttacg atatcgccca
     5761 caatatggga aagattgaga cccatgagat cgatggggag cgcatcaagg tgtgtgtgca
     5821 ccgcaagggt gcgacccgcg ctttcggccc tggtttcgaa ggcttgccgg gcgaattccg
     5881 cgcaattggg cagccggtgc tcgtgcctgg tagtatgggg acagcctctt gggtgctggt
     5941 agggacacag ggcagcatgg agcgttcctt tggctccact tgccacggag ccggcagggt
     6001 gatgagccgg gcacaggcca agaagaaagt gtggggcgaa gacttgcgca accagttgga
     6061 atccggcggc atccacgtgc gagcaggctc gatggcggga ttggcggaag aagccccgca
     6121 agcctataaa gatgtggatg atgtcgttga aaccgtttca ggggctggca tcgctcgaaa
     6181 ggtcgctcgc ttggtccctg tggcagtagt caagggctga gattcgggtg cggggtttca
     6241 gagattctgg atagtatatt aagaggtgga gatgaatcac aagatactgg tttcactcgg
     6301 gttatgtttg cttgctctgg cgttggcagc ctgcgcagga ttgagcgatc cggtggcaac
     6361 agagccaact attgacccct ccgaaccgat ttcca
//
LOCUS       DBNHOH010000837         1594 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1270900, whole genome shotgun sequence.
ACCESSION   DBNHOH010000837 DBNHOH010000000
VERSION     DBNHOH010000837.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1270900"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1594
                     /locus_tag="AC16V7_15095"
     CDS             <1..>1594
                     /locus_tag="AC16V7_15095"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6258497.1"
                     /translation="IAPDLRIKYPDLFPNPPLEPQSEQQRIFESLAAWLAELNSTAPL
                     LLFVDDIHWADSGTLALLRYLARRSTKLRLLILLTYREIELDETSALQSLLYDLNHER
                     ISSRIKLTRFDRQQTRALLASILSPQGEIDDPLLDKIFLETEGNPFFIEEVCKILIEE
                     EKLEHNNGVWSAPTLGEIDIPQSVRLTLQTRLSRLPDQTQEVLRCAAILGRQFDSDTL
                     QRACGLDEESLIIALEKAERAQIISEIPGKRGPAISFAFAHTLIPFTLTDQFGGLRRQ
                     RLHRDAASAIESLRPNDYESLAYHYERAGDAIRAQSYFILAADRALERYANEKAERYY
                     RSALEIEENTPEISQILSGLGEALFRQSRYEEATNYWQKAIDRYRQHGETDQQARLTA
                     RLARAAWYMGDLPKGLEICRQGLAAIPNYKELESPGVASLIHETARAYFFNDMPEEAL
                     PLCQHALDLARRLGLVEVQAETLATLGILPNQPREDAVQALLQAVELSESAGLLAPAV
                     RAHANLGTHMQNIGEFISAREHL"
BASE COUNT          411 a          448 c          370 g          365 t
ORIGIN      
        1 attgcacccg acctgcgaat aaaataccca gatctttttc caaacccacc cttagaacct
       61 cagtcggagc aacagcgtat cttcgaaagc ctggcagcat ggctggctga attgaattca
      121 actgcacccc tcttgctctt cgttgatgat atccattggg cagattcggg cacacttgca
      181 ttgttgcgtt atctggcgcg gcgttcgacc aaactgcgcc tgcttatcct gctcacctac
      241 cgagagatcg aactggatga aacaagcgcc ctgcaatcat tgctttacga tctaaatcac
      301 gagcgcatct cttcgcgcat aaaattaacc cgcttcgatc gccaacaaac ccgggcttta
      361 ttggcgtcta ttctctcacc ccagggtgag atcgacgatc ccctgctgga taaaatcttc
      421 cttgagacag aaggcaatcc cttctttatc gaggaggtat gcaaaatcct aattgaagaa
      481 gaaaaactgg agcacaataa cggcgtctgg tccgcgccca cattgggcga gatcgatatc
      541 ccccaaagcg tgcggttgac cttgcaaacc cgtctctctc gtctgcccga ccaaactcaa
      601 gaagtactcc gctgcgctgc catattagga cggcagtttg actcagacac ccttcaaagg
      661 gcatgcgggc ttgatgagga aagcttgatc atagcattgg agaaagctga gcgtgctcag
      721 atcatctcgg aaataccggg aaaacgcggc cctgctatct cctttgcttt cgcacataca
      781 ctcattcctt ttactcttac agatcaattt ggtggtctgc gccgccagcg attacatcga
      841 gatgccgcat ccgccatcga atcactacga ccaaacgact acgaatctct ggcttatcac
      901 tatgagcggg caggggatgc aatccgcgcc caatcctact tcattctagc tgccgataga
      961 gctttagagc gttatgcgaa cgaaaaggct gaacgctact accggtcagc cctggaaatt
     1021 gaagaaaaca caccggaaat ttcacaaatt ctctctggac tgggagaagc gttattccgc
     1081 caaagccgtt atgaagaagc tacaaattac tggcagaagg cgattgaccg ttatcgtcag
     1141 catggcgaga cagatcaaca agcgcgcttg actgcccgat tagcacgcgc cgcttggtat
     1201 atgggagacc tccccaaagg gttggaaatt tgccgccagg gtttggctgc cattccaaac
     1261 tacaaggaac ttgagtctcc aggagttgct tccctaatcc acgaaacggc ccgcgcctat
     1321 tttttcaatg acatgccaga agaagcccta cctttatgcc agcacgcact agatttggcc
     1381 aggcgtcttg gccttgtgga agtgcaagcc gaaacgcttg ccacgctcgg tatactgcct
     1441 aaccagccgc gtgaagatgc cgtgcaagcg ctgttacaag cggtagagct atccgaatcc
     1501 gccgggttac ttgccccagc tgtgcgagct cacgccaatc taggcacaca catgcaaaat
     1561 attggagaat ttatcagcgc acgtgagcat ctaa
//
LOCUS       DBNHOH010000838         3195 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_565077, whole genome shotgun sequence.
ACCESSION   DBNHOH010000838 DBNHOH010000000
VERSION     DBNHOH010000838.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_565077"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..149)
                     /locus_tag="AC16V7_15100"
     tRNA            complement(77..149)
                     /locus_tag="AC16V7_15100"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Arg,seq:tcg)
     gene            complement(183..1445)
                     /locus_tag="AC16V7_15105"
     CDS             complement(183..1445)
                     /locus_tag="AC16V7_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3179 domain-containing protein"
                     /protein_id="HIR6258498.1"
                     /translation="MNRGRVLALLFFTGFLLSACAGALASGDNPDLPKETSPTENISP
                     TEQVTSPPQITVTPKSIEQTKPLLPEETPPFGATREFSTDFQKHTVPYAEILSGGPPK
                     DGIPAIDNPQFVRIGEADSWLEPLEPVVLIEVGDQARTYPIQILMWHEIVNDTVGELP
                     LTVTFCPLCNTAIAFERKVGDKLLDFGTTGRLRYSNLIMYDRQTETWWQQANGEAIAG
                     ELTGTRLKFYPAAMISWQEFKSIHPNGLVLSRETGFNRSYGRNPYTGYDNINNPPFLY
                     DGPETPGVLPPVARVLTVDLNGEAVAYPYDTLQQVQVVNDQVGGEAIAVFWAAGTASA
                     LDSSSVASGRDVGAANAFKRVLLGRELQFAVVDGKIIDQETGSQWNVLGQAISGELAG
                     KKLDPVVAINHFWFSWAAFKPETRIYQP"
     gene            complement(1663..2376)
                     /locus_tag="AC16V7_15110"
     CDS             complement(1663..2376)
                     /locus_tag="AC16V7_15110"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6258499.1"
                     /translation="MDFHDRVAVITGATGGLGRVVARKFGETGARLALLSSNQDNLNR
                     LVDELSLPADRYMTKALDFRNPDAAKIAAQATVDKYGRADILVHLIGGWTGGKPLVEV
                     EPNQLEEMIQQHVWTTFYLAQSFVPVFHANHWGRMVVISSPNAGMPSAKSAPYAAAKS
                     AQEALILALAQEVKGSGVTANIIRVQTIDVKHQRDQEPSPKNAFWTTPDEIAAAIFFL
                     CSDEGGMVNGARIPLYGSP"
     gene            complement(2379..3137)
                     /gene="ribA"
                     /locus_tag="AC16V7_15115"
     CDS             complement(2379..3137)
                     /gene="ribA"
                     /locus_tag="AC16V7_15115"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HIR6258500.1"
                     /translation="MSLTHMQIKELLDENRDHSCEGFGLDHICVKIAAIADLPSRFGK
                     FHAVAFYNNRDDKEHAAFVHGDICNAENVPVRVHSECLTGDAIGSLRCDCRDQLETAL
                     TTIGQMENGILLYMRQEGRGIGLTNKLRAYGLQDQGYDTVEANLALGFKDDERDYSVA
                     AHMLHSLKVKSIQLMTNNPRKINELAAYGINIAGRIPLIIPPNPYNQFYLETKAEKSG
                     HLLDPNGKEHLFEQMDRPVLPGMTQEQIEILQEN"
BASE COUNT          739 a          824 c          815 g          817 t
ORIGIN      
        1 aaaagaacct gctaaaaaaa acaggttctt acccccacag gtgggggatc aaaacggcga
       61 caaggctaat tgttagcgcc cccggcacga ttcgaacgcg caaccctttg atccgaagtc
      121 aaatgctctg tccattgagc tacgggggcg tctgcctgaa attatactcg acagaaggcg
      181 attcagggct gatatatcct ggtctccggc ttgaaagccg cccaggagaa ccagaagtgg
      241 ttgatggcga cgactggatc cagtttcttg cctgccagct caccgctgat ggcctgcccg
      301 agtacattcc attggctgcc agtctcctgg tcgatgattt ttccatcgac cactgcgaat
      361 tgtaattccc gcccaagtaa aacccgtttg aaggcgttcg ctgctccaac atctctcccg
      421 ctggcgacag aactgctatc tagggctgag gccgtaccgg ctgcccagaa aaccgcgatg
      481 gcttcaccac ccacctggtc gttgacaacc tggacttgct gaagcgtatc ataagggtag
      541 gctactgcct cgccgttcag atccaccgtc agcacgcgcg ccacaggggg caacacgccc
      601 ggtgtttctg ggccatcgta caagaacggc gggttgttga tattatcata accggtgtag
      661 gggttgcgac cgtacgaacg gttgaaaccg gtttcgcggg agagcaccag accatttgga
      721 tgaattgatt taaattcctg ccatgagatc atcgcggcag ggtagaattt caagcgcgtt
      781 ccggtcagct cgccggcgat ggcctctcca ttagcctgct gccaccacgt ttctgtctgg
      841 cgatcgtaca tgatcagatt gctgtagcgc aatctgccgg tggtgccgaa gtccagcagt
      901 ttatccccga cttttcgttc aaaagcgatg gcggtgttgc acaaggggca aaaagtgacc
      961 gtcaggggaa gttccccaac cgtgtcgttt acaatttcgt gccacattaa gatttggata
     1021 gggtaggtgc gcgcctggtc tcccacttcg atcaggacca cgggctccag cggttcaagc
     1081 cagctatccg cttccccaat gcggacgaat tgcgggttgt cgatggctgg tatgccgtcc
     1141 ttcggaggtc caccggaaag gatttcagca tagggaaccg tatgcttttg aaaatcggtc
     1201 gaaaactccc tcgttgcacc aaagggaggc gtttcttcgg gcaataatgg ctttgtctgt
     1261 tcaatacttt ttggtgtgac cgttatttgt gggggtgatg taacctgctc agttggggat
     1321 atattctctg ttggtgaagt ctccttgggt aaatcaggat tatcccctga tgctaatgct
     1381 cccgcgcacg cagagagcaa gaaaccagta aaaaatagta gagccaaaac tcgtcctctg
     1441 ttcattaatg tctccatcct atacttgaga tactgatatg cttatcctag aaatcccgtt
     1501 ttgtcgggat ttccttgttt aattagatag caagaaggat agaaatatta ccaaaataaa
     1561 agtaactgct ctcagcgcaa gctgctggag cagttacgaa agcgtaccgt aaaaacaact
     1621 agggattgtg cagttccaaa ccgctataaa cgaggttgat gctcagggac tgccatagag
     1681 tggaatacgc gccccgttca ccatcccgcc ttcgtcggaa cacaaaaaga aaatggctgc
     1741 cgcgatttcg tctggagtcg tccaaaatgc gttctttgga ctgggttcct ggtctcgttg
     1801 gtgcttcaca tcgattgtct gaacgcggat gatgtttgcg gttacaccgc tgcccttgac
     1861 ctcttgggcc agcgccagga tcaatgcttc ctgggcggat tttgcagcgg catacggggc
     1921 gctcttggca gatggcatgc cagcattggg ggaagaaatg actaccatgc gcccccagtg
     1981 attggcatga aagacgggca cgaatgactg tgccaggtag aaagtggtcc agacatgctg
     2041 ctggatcatt tcctccaatt gattgggttc tacttcgaca aggggcttgc ccccagtcca
     2101 gccaccgatg agatgcacga ggatatctgc gcgaccatat ttatccaccg tcgcctgcgc
     2161 tgcgattttt gccgcatctg gattgcgaaa gtccagtgct ttcgtcatat aacgatccgc
     2221 aggaagactc agttcatcaa caagccgatt gagattgtcc tggtttgagc tcaatagtgc
     2281 cagccgcgca cctgtctctc caaacttgcg ggctacaacc cgccccaggc ctcctgttgc
     2341 tccagtgatc acagcgacgc ggtcatgaaa atccatgttt aattctcctg cagaatttca
     2401 atctgttctt gcgtcatgcc aggtagtact ggtcgatcca tttgctcaaa taagtgttct
     2461 ttaccatttg ggtccagcaa atggccagat ttttcggcct tggtctctag ataaaattgg
     2521 ttataggggt tgggcggtat gatcaatggg atgcgtcccg cgatgttgat gccatacgca
     2581 gccagctcat taatcttgcg cgggttattg gtcatcaact ggattgattt gacttttagc
     2641 gagtggagca tgtgtgccgc cacactataa tcacgctcat catccttgaa gccgagcgct
     2701 agattggctt caacggtgtc ataaccttga tcttgcagac cgtaggcacg caacttattg
     2761 gtcaatccga tcccgcgccc ttcctggcgc atatacagca gtatgccgtt ttccatttga
     2821 ccgattgtgg tcaaagctgt ctcaagctga tcacggcagt cgcaacgcaa cgagccgatg
     2881 gcatcccctg tcaggcattc tgaatgcacc ctgacaggta cattctccgc attacagata
     2941 tccccgtgca cgaatgcggc gtgttcttta tcatcccgat tgttgtaaaa tgccactgcg
     3001 tgaaatttgc cgaatcgcga aggcaggtcg gcgatggcag ctatcttgac acaaatgtga
     3061 tccaggccaa aaccctcaca agagtggtcg cggttttcat ccaataattc ttttatttgc
     3121 atatgagtga gtgacatacg gtctcctatt ttacgaaaaa ttctattggg cttaattaac
     3181 tcggtgttct cgtgt
//
LOCUS       DBNHOH010000839         4727 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1464481, whole genome shotgun sequence.
ACCESSION   DBNHOH010000839 DBNHOH010000000
VERSION     DBNHOH010000839.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4727)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1464481"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="AC16V7_15120"
     CDS             <1..893
                     /locus_tag="AC16V7_15120"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HIR6258501.1"
                     /translation="ALSLDWRVFFRYTIATFLMDMARQPQVGDQGCTTWAVLSEATRD
                     GIPLLVKNRDYWPDHQELQCLAHTRPDKGYVYLNMTSAGSPGVFSSGINEAGLAVSDT
                     HVSSLDIGPGLPRYYLMMEILEHHDTVHSAVDYLRSVNHAGNGTLLLLDANGEAAVFE
                     CGQIFSKSIPATNGFVVSANHYVTPALRQKWMFRTTDRKLIGSSLHRYSRVKRALLKA
                     KGSIDLNWAINLMRQHGTPLNAICRHPELNPQSVTISSAIYLPTKKQLYLVNGIPCQN
                     EYQVYPVIPDVQDEKQAYAL"
     gene            complement(996..3320)
                     /locus_tag="AC16V7_15125"
     CDS             complement(996..3320)
                     /locus_tag="AC16V7_15125"
                     /inference="COORDINATES: protein motif:HMM:NF012544.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIR6258502.1"
                     /translation="MNSIAPNSTRSIPEKVTLSRSLGLFTITMIGVGGMIGAGIFVLT
                     GIAAGVAGPALILAFVLNGLVTILTGMVYAELGSAFPEAGGGYLWVKEGLGGAQGFLA
                     GWMSWFAHAVAGSLYALGFGRFFSELWVLAGVATFNLEVEQMTLIFMTLIIAAFTYLN
                     YRGASETGTIGNIITMTKILILGVFVLFGVLAMLRTDAWLTRFTSGFMPNGMLGVIIA
                     MGLTFIAFEGYEIIAQTGEEVINPRKNVPRAIFLAIILVVVIYVFVGVTAIGAVTPPQ
                     GMKAYEYLGEQGEIAVVGVAAQIFPLGVGAVIMLVSGLVSTTSALNATTYSSSRVSFA
                     MGRDHNLPSIFARIHPLRHTPYLAVIASGALMLLIAWILPIEEVAAAADIMFLMLFLQ
                     VNLAVMTLRHKMPDLERGYRVPWYPFIPILALFSNAILALSLFSYAPVSWYTAIAWVI
                     FGLLIYYTYFSKIEAMEKPKEILMEEVLVTRDYSIVVPVATQEQAMILGQIGAILAKV
                     NNGEVLALHVVRVPRQFTLGEGRLFLKEGRSYLETVIHRAKPFDVPVHTVIRLGRSVA
                     EAVHKTIDESASDLLVLGWPGYTHTAGRIYGSVIDPIIDNPPTDIAIVRYRQRRSIKK
                     ILVPVAGGPNSRLAVKLAVDMAMSGEGGPALVSLLHIIPFGARNGAIVRAQQAFRESM
                     SGIAYENIEKRVVEGSNIIETILSEAAGDEDTPQSDLIVIGATLEPLLKKLRMGNISE
                     QVARRASVTVVIVKRRSGPLHSFLRQTVMEPTRI"
     gene            complement(3343..>4727)
                     /locus_tag="AC16V7_15130"
     CDS             complement(3343..>4727)
                     /locus_tag="AC16V7_15130"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012356.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HIR6258503.1"
                     /translation="DIFRMGKRFRLMFDAVINHISAQSAWFQGFLQDQPAYRDYFIVV
                     EGQPDLSQVVRPRALPLLTSVSTSSGEKSVWTTFSTDQIDLNYQNPQVLLEIVDTLLF
                     YIEKGAEFIRLDAIAYLWKEIGTSCIHLPQTHRVVQLFHSILDIMAPQVQIITETNVP
                     HDENISYFGDGENEAQMVYNFALPPLVLHTMQTGDARAISRWAAGLKLPSDQVTFFNF
                     LASHDGIGVTPAKGLISPEEINMLVERALAHGGLVSYRSNPDGSQSPYELNINYFDAL
                     SDPKGGELLQKQVKRFIAAHAIMFSLVGVPGIYFHSIFGSRGWLEGVAITGRNRTINR
                     QKLDLTELKAGLSDPGSTRSMVNEGLARLLKARAAHPAFHPYGQQRILDLGARIFCIL
                     RTSPDGRSRVVCLQNVSDQVVQVGLPLEGLAFDPTSLPIDIITQGKVELSSPGRLSLQ
                     PYQVLWMADI"
BASE COUNT         1245 a         1268 c         1143 g         1071 t
ORIGIN      
        1 aagctttaag tttggattgg cgggtcttct tccgctatac cattgccaca tttttaatgg
       61 atatggctcg ccaaccgcaa gttggcgatc agggatgcac cacctgggca gttctatcag
      121 aggccacccg tgatggcata ccgttattgg taaaaaaccg cgattactgg cctgaccacc
      181 aggaactgca gtgcctggcg catacccgtc cagataaagg ttacgtatac ttgaacatga
      241 ccagtgcagg tagccccggc gtctttagct ctggcattaa cgaagccgga ttggcggtct
      301 ccgatacgca tgtatcatca ctggatattg gcccaggatt gcctcgctat tacctgatga
      361 tggagatttt agagcatcac gacacagtgc actcggcggt tgattacctt cgctcggtca
      421 atcatgcggg gaatggcacg ctgttattac tcgatgccaa cggggaggca gcagtatttg
      481 aatgcggtca aattttttcg aaatcaatcc cagccaccaa cggatttgtt gtcagtgcca
      541 accactatgt aaccccagcc ctgcgccaaa aatggatgtt tagaaccaca gacagaaagt
      601 tgataggcag ctccttgcat cgctactccc gcgtgaagag ggcgttatta aaagcgaaag
      661 gcagtattga cctcaactgg gcgatcaact taatgcgaca acacggtacc ccattgaacg
      721 cgatttgccg ccaccccgag ctgaacccac aatcggtgac gatttccagt gcaatctacc
      781 taccaacaaa aaaacagctt tatctggtta atggtattcc ctgccaaaat gaataccagg
      841 tatacccggt tattccagac gttcaggacg aaaaacaggc ctacgctctc tgaacgccct
      901 tttttacagg actgttcgaa tttattcccc ccaattgcac aattcctgaa tttactcggc
      961 ctagatcatc gctatgccct aaggggctta aattgttaga tccgtgtagg ctccatgact
     1021 gtttggcgca ggaatgagtg cagtggaccg cttcgccgct tgacaatgac caccgtaacc
     1081 gatgccctgc gagccacttg ttcagatata ttccccatgc gcaacttttt tagcaagggc
     1141 tctaatgtcg ccccaatgac gatgagatca gattggggcg tgtcttcatc acctgctgct
     1201 tcgctcagta tagtctcgat gatattgctg ccttcaacca cgcgcttttc gatattctca
     1261 taggctatac ctgacatcga ttctcgaaaa gcctgttgcg ctcgcactat agccccattg
     1321 cgcgcgccga atgggatgat gtgcaacaac gaaaccaacg ccggtccacc ttcaccagac
     1381 atagccatat cgacagccag cttgacagcc aggcgactgt ttggccctcc agcaacaggg
     1441 acaaggatct ttttaatgct gcggcgctgg cgatagcgca cgatggcaat atccgtgggc
     1501 gggttatcaa taatcggatc aatgaccgag ccatagattc tccccgcagt atgggtgtac
     1561 cccggccatc ctaaaacaag caggtccgat gcgctctcat caattgtttt gtggacggcc
     1621 tcggctacac tgcgccccaa acgaatcacc gtatgcacag gcacatcaaa tggttttgca
     1681 cggtgaatca cggtttctag gtaagaccga ccttctttca aaaatagacg cccctctccg
     1741 agggtgaact ggcgtggcac ccgtactaca tgcaaggcca gcacctcgcc gttgttgact
     1801 tttgctaaaa ttgccccgat ttgacccagg atcatcgcct gctcctgtgt agcgacgggt
     1861 accacgatgg agtaatcccg ggtgaccaga acttcctcca tcaatatctc tttgggtttc
     1921 tccatggctt caattttgga aaaatacgtg taataaatca gcaatccaaa gatgacccag
     1981 gcgatggccg tataccagga cacgggtgca taactgaaca aagataacgc caggattgcg
     2041 ttggagaata atgccaaaat aggaatgaaa gggtaccagg ggactcgata cccccgctct
     2101 aaatcgggca ttttgtgtcg cagtgtcatg acagctagat tcacctggag gaaaagcatc
     2161 aagaacatga tatccgccgc ggctgccacc tcttcgattg gcaatatcca agcgatcaaa
     2221 agcatgagtg cgcccgaagc gatgaccgcc aagtaaggcg tatgccggag cgggtgaatg
     2281 cgggcaaaga tagatggcaa gttatgatcg cgtcccattg caaaggagac tcgtgaagaa
     2341 gaataggtgg tagcgttgag cgccgaagtg gtcgaaacga gaccacttac caacatgatg
     2401 actgccccta ccccaagtgg aaaaatctgc gccgcaacac caacaactgc aatctctccc
     2461 tgttccccca ggtactcata cgccttcatg ccctggggcg gggttaccgc cccaatggca
     2521 gtaactccaa caaacacata aatgaccacg actaaaatta tggctaaaaa gatcgcccgg
     2581 ggcacgtttt tccgagggtt tattacctct tcaccagttt gcgcaataat ctcatatccc
     2641 tcgaaagcga tgaatgttaa ccccattgca atgattaccc ctagcatgcc atttggcatg
     2701 aaaccgctcg taaagcgcgt gagccaggca tcggtgcgca gcatggcaag cacaccaaac
     2761 aaaacaaata cccccaagat caggatcttt gtcatggtga tgatattacc gattgtcccg
     2821 gtctcagaag cgccccgata gttaagatat gtaaaggcgg cgatgatgag ggtcataaat
     2881 atcaaggtca tctgttcgac ctcgagatta aaggtggcaa cacctgccag aacccagagt
     2941 tcagagaaaa atctgccaaa tcctagagca tataaactgc cagccactgc gtgagcgaac
     3001 caactcatcc agcctgctaa aaatccctgg gcaccgccta ggccctcttt cacccagaga
     3061 tatccgccgc ccgcttccgg aaatgccgag cccaattcag cataaaccat gcctgtcaat
     3121 attgtcacca gcccgtttag aacaaacgcc aggatcagcg caggacccgc tacgccagcc
     3181 gcgatgccgg tgagcacaaa aatacctgcg ccaatcatgc cgccgactcc gatcatggtg
     3241 atcgtgaata agccgaggct gcgacttaaa gtaactttct cagggatgga tcgtgtggaa
     3301 ttgggagcaa tcgagttcat ggatcgcttg tccctttcga gtttagatat ccgccatcca
     3361 caaaacctgg taaggctgca gtgaaagcct gccaggggaa ctcagctcca cctttccctg
     3421 tgtgatgatg tcaatgggta gggaggttgg gtcaaatgcc aaaccttcaa ggggcaatcc
     3481 aacctggacg acttggtcgg atacattctg caggcagacg acacgtgacc ttccatctgg
     3541 tgatgtgcgc aagatgcaaa agatcctggc gccgagatcc aaaatccgtt gctgtccata
     3601 agggtggaag gccggatggg cagcacgtgc tttaagcaag cgagccaggc cctcattaac
     3661 catggagcgg gtggaaccag ggtctgaaag accagctttt aactccgtca aatcaagttt
     3721 ctggcggttg atcgtccggt ttctaccggt tatcgccacg ccctccagcc agccacgcga
     3781 gccaaaaatg gagtgaaaat aaattccggg cacacccacc agggaaaaca tgatcgcatg
     3841 ggcagcgatg aagcgcttaa cctgtttctg caaaagttct ccacccttag ggtccgatag
     3901 tgcgtcaaag taattgatat tgagctcgta cgggctttgg ctgccatccg ggttcgagcg
     3961 gtatgatacc agccctccat gggctagcgc ccgctcaacc agcatattta tttcctcagg
     4021 agagatcagc cctttagccg gtgtaacgcc aatgccatca tgcgaagcca agaaattgaa
     4081 aaaagtgact tgatctgatg gcagcttcaa tccagccgcc caacgcgaga tcgcccgagc
     4141 gtccccggtt tgcatggtgt gcaagaccaa tggtggcaaa gcgaaattat agaccatctg
     4201 ggcttcattc tccccatcgc caaaataaga aatgttctcg tcatggggta cattcgtctc
     4261 ggtgatgatc tgcacctgag gtgccatgat atccagtatg ctgtgaaaca actgtaccac
     4321 ccgatgggtt tgaggcaggt gaatgcaaga ggtaccaatc tctttccaga gatatgcaat
     4381 cgcatccaga cgaatgaact ctgcgccttt ctctatgtaa aagagcaggg tatccacgat
     4441 ttcaagcaag acctgtgggt tctggtaatt caggtcgatc tggtcagtgc tgaaggtggt
     4501 ccacacggat ttctcgccag acgaagtgct gaccgacgtt agcaagggca gcgcccgcgg
     4561 cctgacgact tgtgataggt ccggctgacc ttcaacaact atgaaataat ccctataagc
     4621 aggttggtcc tgtaaaaacc cttgaaacca cgcactttga gctgagatat ggttgatcac
     4681 cgcatcaaac atcaaacgga atcgttttcc catcctgaat atgtcat
//
LOCUS       DBNHOH010000840         4461 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_53407, whole genome shotgun sequence.
ACCESSION   DBNHOH010000840 DBNHOH010000000
VERSION     DBNHOH010000840.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_53407"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="AC16V7_15135"
     CDS             complement(<1..1020)
                     /locus_tag="AC16V7_15135"
                     /inference="COORDINATES: protein motif:HMM:NF039823.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HIR6258504.1"
                     /translation="MDQDLDIILAESFQILRTEHYRSSQKAAIGRANFLAQAKSLAQE
                     ERVGVPKLRFPRLSPGTWIGMTPPMHTRKLSPRLLVVIGSWILIFTIFFGTVSASVYA
                     SQDSLPTDPLYPIKIFGEEIHLALILDFDERTVLLLRYSRYRLDEMLEMVANQEPFPE
                     KTALNWQEHINLALHTAAQMDDAAMMRALEHIDLALRLQEQRLQAIQAGGIIELDGLH
                     VWLRQLEVDVHNGMQDPAAFRQRWQTPYPGGQIPLDDHDQITPEVTLPRSLQVKPLRV
                     DPFQSSTDQLNQIRSRKPGDSGATSATTPDPGQLKPSCDKDCIPTIDQVGSEGEPNPM
                     PIHENE"
     gene            complement(1029..1577)
                     /locus_tag="AC16V7_15140"
     CDS             complement(1029..1577)
                     /locus_tag="AC16V7_15140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6258505.1"
                     /translation="MISEEQLCAQIHDFDEHILAEVYDRYSPEIFRYTMRLLGDSELA
                     EECVAETFHRFLLAIKRGGGPRQYLRAYLYRVAHNWVSDYYRNQNKSALPLDPDCCID
                     EHKQPPHVFAEESMRRDVRLALSKLTPDQRQVIVLRFLEDWEYADISNALDKPVGAVK
                     ALQHRGLKALRRHLLKEEAMIA"
     gene            1650..2969
                     /locus_tag="AC16V7_15145"
     CDS             1650..2969
                     /locus_tag="AC16V7_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIR6258506.1"
                     /translation="MFRDKKIAASKQSTFARWISSLYSLLSGQGARQGYLAAVDQGVI
                     SASNFLATIILARSITPTELGVYGVGFIALRLVRSLQEGLVVQPMNVYGAGMDEHSFR
                     RYATSSSVLQISLAASSSLVAAISGWLLTALGNDTAGPALFSLWFAFLWWQLQEYIRR
                     MLYTRGMVFSAVVNTIFANAFRIFLLLWWAKQGKLTGILGLDAIAWGSLVALLPGLWA
                     TRSYWVRDFPNLKQTWLRNWGLGRWLLGSSLANWVAVEFYPVLTAGMISFAAAGAYRA
                     LQNLVAPIHLLLRATDTFLTPRAARLYELNGQTAMRRTLRLIYLISGIPILGVLAIAI
                     LFPTPLLRLLYGETYLAYSSSMALMGLFYALLYAYYPLQTAFKAARLTRPIFLANLGA
                     IAAMFTIGIWAILRWGVAGTIAGQTLNALIVSIILWGTWIRILRRQN"
     gene            complement(3014..4141)
                     /locus_tag="AC16V7_15150"
     CDS             complement(3014..4141)
                     /locus_tag="AC16V7_15150"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR6258507.1"
                     /translation="MGKGQIEESQSHSLHTGRQTITNALALFIAAFVLLFATWLYAQV
                     LWNEASWRNVIGRFILHALLTLILERALAITSFPRDWRYRAAFLLSIGVYLGYTGLQP
                     MNMAWVVNYTNLSLVVLGSFSGGLLATGLNESLWEENSPPSQLIRMEVHQHHLDKIGD
                     PDPEPLLKRTFDVSLALLGLVLSLPVWLMGILVIWLEEPGPLLFVKNSVGKGGINFHQ
                     YKFRTMVREAEYSTGPVLASESDKRVLVVGRFLRKTALDELPQLINILLGQMSFVGPR
                     PQRTVLVRGYLESIPGYEERHRVLPGLAGLAQVAGDYYLTPHQKLRFDRLYIRHRSIG
                     FDLKLIILAFSIAFWFRWQDGWNGRLPRKWFRWASPYQPPA"
     gene            4189..>4461
                     /locus_tag="AC16V7_15155"
     CDS             4189..>4461
                     /locus_tag="AC16V7_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258508.1"
                     /translation="MRHPQQKTLIALAASAILVAAAAGALIFPKAIQKWSAPLGPSLE
                     LPTHTPTMESLVREIPTPTDVPATQITAVEPVSRKDTPIAVPTLSTS"
BASE COUNT         1042 a         1175 c         1133 g         1111 t
ORIGIN      
        1 ctcgttttca tgaatcggca tgggattcgg ttcaccctca gagccaactt gatctattgt
       61 tggaatacaa tctttatcgc acgaaggttt aagctggcca ggatcgggcg tagttgcact
      121 ggttgcgcct gagtcacctg gcttcctaga tctaatctga ttcagctggt cagtcgacga
      181 ttggaatgga tccactcgta gaggtttaac ctggagggag cgggggagag ttacctctgg
      241 ggtaatctgg tcgtgatcat ctagtggaat ttggccacct gggtacggag tctgccagcg
      301 ctggcgaaag gctgctgggt cttgcatgcc gttgtggaca tctacttcaa gctgacgcag
      361 ccatacatgt agcccatcca gctctataat cccaccggct tgaatggctt gcaagcgctg
      421 ttcttgcagt ctaagcgcca aatctatatg ctctaaagcc cgcatcatgg cggcatcatc
      481 catttgagct gctgtatgga gagccaggtt gatatgctcc tgccaattaa gagctgtctt
      541 ttctgggaac ggctcttggt tggcaaccat ctcgagcatc tcatctagcc ggtatctgct
      601 atagcgaagc aacaagaccg ttcgctcatc aaaatctagg ataagcgcga ggtggatttc
      661 ctccccaaaa atctttatag ggtatagagg atcggtgggt aggctgtcct gtgaagcata
      721 aacgcttgcg cttacagtcc caaagaagat ggtgaaaatg agtatccagc tgccaattac
      781 aactaataga cggggagata attttcttgt gtgcattgga ggggtcatac cgatccatgt
      841 acctggacta agcctcggga atctcaattt gggtactcca accctttcct cctgggccaa
      901 actctttgcc tgtgcgagaa aattcgcccg tccaatggct gctttttgag aagagcgata
      961 atgctcggtg cgcaagattt ggaagctctc agccaaaatt atgtctaaat cttgatccat
     1021 tggaattctc atgcgatcat cgcctcttct ttcagcaagt gtcgccgcaa ggctttcaac
     1081 ccgcggtgtt ggagcgcttt tactgcgcca acgggtttat ccaaagcgtt agaaatatct
     1141 gcatactccc aatcttccag gaagcgtagc acgatcacct ggcgttggtc tggggtgagt
     1201 ttcgataacg ccaagcgtac atcccgtcgc atcgactcct ccgcgaatac atgtggaggc
     1261 tgtttgtgct cgtctatgca acagtccgga tctagaggga gcgcactttt gttctggtta
     1321 cggtaataat cgctaaccca gttatgggcc acacggtaaa gataggcgcg cagatattgg
     1381 cgtggcccac cgccgcgctt tatggcaagc agaaatcgat ggaaagtttc agcaacgcat
     1441 tcctcagcaa gctctgaatc gcctagaagc cgcatcgtat accgaaatat ttccgggctg
     1501 tagcggtcgt aaacctcagc caggatatgc tcatcaaaat catggatctg cgcgcagagc
     1561 tgctcttcag aaatcattat cctttatttg tgacgcaaaa ctagtaaaaa tgatacggct
     1621 cacaaggaaa ttcgattaga attggcgtat tgtttagaga caagaaaatc gccgcaagca
     1681 agcagtcaac attcgctcgc tggatcagct cgctttacag cctactctcc ggtcaagggg
     1741 cccgacaggg ttatcttgcc gcagtagacc agggcgtaat ttccgcatcg aactttctgg
     1801 caacaatcat cctggctcgc agcatcaccc caacggagct gggcgtgtat ggggttggct
     1861 ttattgccct gagattggtg cgttccctcc aggagggttt ggtggtgcaa ccaatgaatg
     1921 tctatggggc agggatggat gagcattcat ttcgccgcta tgccaccagc tcgtctgttc
     1981 tgcaaatctc gctggcggcc tcctcatcgc tggtggcggc gatctcgggc tggctgctga
     2041 cagcgctggg aaatgatact gctggtcccg cacttttttc gttgtggttc gcattcttgt
     2101 ggtggcaatt acaggaatat atccgccgca tgctctacac gcgtggcatg gtcttcagcg
     2161 ctgtggtcaa taccatcttt gccaacgcct ttcgcatctt cctgttgcta tggtgggcaa
     2221 agcaaggcaa attgacgggt attcttggct tggatgccat cgcttgggga tcgctggtag
     2281 ccctgcttcc cgggctttgg gcaacacgct catattgggt tagagacttc ccgaacttga
     2341 aacagacctg gctccgcaat tgggggttag gtcgttggct cttaggcagc agcctggcga
     2401 attgggtagc cgtggagttc tatccagtcc tgaccgctgg catgatcagt tttgcagcgg
     2461 ctggcgcgta ccgcgccttg cagaatctcg ttgcgcctat ccacctgctc ttgcgagcca
     2521 ccgatacctt cctgacccca cgcgcagccc gcctatatga gctgaacgga caaacggcga
     2581 tgcggcgcac gctacgcctg atttacctga tatctgggat tcccatattg ggcgtcttag
     2641 ccattgctat tctcttccct acgccgttgt tacgactgct ctatggagaa acttatttgg
     2701 cctatagcag cagcatggcc ttgatgggtc tcttctacgc tctgctgtat gcctactacc
     2761 ccctgcagac agcattcaaa gcggcacgcc taacccgccc tatctttctt gccaacctgg
     2821 gagccatcgc agccatgttc actatcggca tctgggctat cctgcgctgg ggagtggctg
     2881 ggaccatcgc aggtcagact ctgaatgcct taattgtttc aattattctt tgggggacgt
     2941 ggattaggat actacgccgg cagaactgaa aatcaggggg atcgcgtcac taccgtttaa
     3001 caaagccaag ggtttaggct gggggttggt atggtgatgc ccagcggaac catttcctgg
     3061 gcaacctgcc attccacccg tcttgccagc ggaaccagaa ggcgatcgaa aaggccagaa
     3121 tgatcaattt aagatcaaat ccaatacttc ggtgacggat gtacaacctg tcgaaacgca
     3181 atttttgatg tggggtcagg taataatcgc cagcaacctg tgccaatcca gccaaaccag
     3241 gcaagacacg gtgcctttct tcgtatccag gtatcgactc caggtagcca cgcaccagta
     3301 ccgttcgctg tggacgtggc ccgacgaagc tcatctgtcc tagaaggata ttgatcaatt
     3361 gtggtagttc atccagtgcg gtcttacgca aaaaacggcc cactaccaga acacgcttat
     3421 cactctcgct tgccagcacc ggaccagtgc tgtattcagc ttccctgacc attgtcctga
     3481 acttgtattg gtgaaaatta attccgcctt ttcctactga gttctttaca aacagcagcg
     3541 gtcccggttc ttccagccag atcacgagga tacccattag ccacactggc agagataaca
     3601 ccaagcctaa gagcgccagg gatacatcaa aggttcgttt gagcaggggt tcggggtctg
     3661 ggtcgccgat cttatccagg tgatgctggt gcacttccat tcggatgagc tgagaggggg
     3721 gcgaattttc ctcccataga ctttcattca agcctgtggc taaaagacct cccgagaaag
     3781 aacccaaaac taccaaagac aggtttgtat aatttactac ccaagccatg ttcattggct
     3841 gcaagccagt gtaacctaga tataccccaa tggagagcag aaaagccgcc cggtagcgcc
     3901 aatctcgggg gaaagaggtg attgccagag cacgctccaa tattaaggtc aatagggcgt
     3961 gcaggataaa gcgcccaatc acattgcgcc aggatgcctc attccagagc acttgagcgt
     4021 agagccaagt agcgaacaga agcacgaagg cggcaataaa taatgccaac gcgttggtaa
     4081 tcgtttgccg acctgtgtgc aacgaatggc tttgagattc ctcgatttgc ccttttccca
     4141 tagtcttgac aatggaaaat tatacctctg atataatcct cgccagatat gcgccaccct
     4201 caacaaaaaa ctctcattgc cctggctgca tccgcaatat tggtcgcggc ggcagctggg
     4261 gctctgatct ttcccaaggc tatccagaag tggagtgcac ccctgggtcc aagtctggaa
     4321 ctgcccaccc atacaccaac catggagagc ctggttaggg aaatccctac acccaccgat
     4381 gtgccagcaa cacaaattac cgctgtagaa ccagtgtcta ggaaggacac tccaattgcg
     4441 gttcccaccc tttctacctc t
//
LOCUS       DBNHOH010000841         2580 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_374240, whole genome shotgun sequence.
ACCESSION   DBNHOH010000841 DBNHOH010000000
VERSION     DBNHOH010000841.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_374240"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1770)
                     /locus_tag="AC16V7_15160"
     CDS             complement(<1..1770)
                     /locus_tag="AC16V7_15160"
                     /inference="COORDINATES: protein motif:HMM:NF020498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HIR6258509.1"
                     /translation="MRKHILKASIPVLIACLLIVVLLVLPGISKIHNNYLGVTGPAAV
                     GMAQTTPITSTVYLPLIVSPGLSTSRVVVDNHQAFDKCALPSLQQMQTWWDKSPYWVH
                     NLYIGGISFACRNNPLDEAWIKAAAKQGWSFILTWVGPQAPCTSYKYRMSSSTSTAYQ
                     QGRSEADLANTAAVNLGITGGKIIYYDVESYSSADTACRNAVKSFMRGWVERLHELGA
                     KAGGYGSPCTSFLSDWATNNPAPDDVWIAYWKLPPEYDPAASVWNVPCLSNSLWTDHQ
                     RLRQYTGGHSETWGGVSMTIDSNVLDGEIVTFKPPPSPSLADGTQSDSQRLGTPQIRD
                     MGTLSPESGWVLAGDRLFLMRQAGPSWQEITPGLDSALGILTVNFLDSQQGWLVRRTQ
                     EPAPWGGLAISRTDDGGSNWQDFPLPAHMSEAPSPVVAAYLDFIDANLGWLALELQTG
                     SSFSLGRLFVTQDGGRTWEERPIPLGEAVKFLDAQRGWVAGGPSGERLYQTVDGGRTW
                     QPQSLSYPDSIASARALVGLPQFQNDLEGVLPVTMSGSPDSHFALFETRDGGESWSLA
                     TRLELDPGVEPASPLPFSLGGGSR"
     gene            2027..>2580
                     /locus_tag="AC16V7_15165"
     CDS             2027..>2580
                     /locus_tag="AC16V7_15165"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR6258510.1"
                     /translation="MQTHWPLSPWVEEHRQHIGFGLQVFPRGVSTERTQQLHALGQLA
                     EGLGYDTFLIGDHPARGPEPWLHLAALAMATQRIRLGSLVSCALYRHPLMTARLLSDL
                     DHLSAGRAILGLGIGWDANEFASLGIPFLPVPERQAALEEALVIIRGACGESHFTYQG
                     RYFQTEQAQVSPPPLQSPFPPILIA"
BASE COUNT          543 a          776 c          734 g          527 t
ORIGIN      
        1 tcggctgccc ccacccaggc tgaatggcaa cgggctggcc ggttctacgc cgggatcgag
       61 ttctagtctg gtcgccaggc tccagctttc gccgccgtca cgagtctcaa acagagcaaa
      121 gtgggaatct ggagagccac tcattgtcac cggaagaacc ccctcaaggt cattctgaaa
      181 ttgcggtaac ccaaccagcg cccgcgcgga ggctatggag tcaggatatg acaagctctg
      241 tggctgccag gtgcgcccgc catccaccgt ttgatagaga cgctcgcccg atggacctcc
      301 ggctacccag ccccgctgag catccaggaa tttaaccgcc tcgccgagcg ggatcggacg
      361 ctcttcccag gtgcggccgc catcttgggt cacgaagagc ctgcccaggc taaaactgct
      421 gcccgtttga agctcgagcg ccagccaccc caggttagcg tcgatgaaat ccagataagc
      481 agccacgacc ggcgaggggg cctcgctcat gtgtgccggc aaggggaagt cctgccagtt
      541 cgagcctcca tcatccgtgc gagagatcgc caggcctccc cacggagcgg gctcttgagt
      601 gcgccggacc agccaaccct gctgggaatc caggaagttc accgtcagga taccaagcgc
      661 gctatccaag cctggcgtga tttcctgcca gcttggccca gcctgccgca tcaagaagag
      721 ccgatcaccg gccaataccc acccactctc aggcgagagg gtgcccatat cccgtatctg
      781 cggggtaccg agacgctggg agtccgattg cgtaccatca gccaaagacg gagagggcgg
      841 gggtttgaat gtcacgattt caccatccaa cacgttgctg tcgatggtca tagatacccc
      901 gccccaggtc tcggaatgtc ccccggtgta ctgtctcagg cgctggtgat cagtccacag
      961 cgagttggaa aggcagggga cattccagac gctcgcagca ggatcatatt caggcggtag
     1021 tttccagtaa gcgatccaga cgtcatcggg tgcgggatta ttggtagccc aatcgctgag
     1081 gaaggaggtg catggggatc catatccgcc agccttcgct cccagctcgt gcagccgttc
     1141 cacccagcca cgcatgaaac tttttacggc attgcggcag gcagtgtcag cgctggaata
     1201 actctctacg tcgtagtaaa ttatcttgcc gcccgttatt ccaaggttaa ccgccgcggt
     1261 attggctaaa tccgcctccg acctgccctg ctggtaggcc gtgctggtgg aagaactcat
     1321 gcgatatttg taggaagtac agggcgcctg cgggcctacc caggttagga taaatgacca
     1381 gccctgttta gctgctgctt tgatccaggc ctcatctaag gggttgttcc ggcaagcgaa
     1441 gctgatcccg ccaatgtaga ggttatgcac ccagtagggg cttttatccc accaggtttg
     1501 catctgctgc agggatggca gggcacactt atcgaaggcc tgatggttat cgaccacgac
     1561 tcgactggtt gataaacccg ggctgactat caagggcagg taaaccgtcg aggtgatagg
     1621 agttgtttgt gccatgccta ctgcggctgg cccggtgact cccaggtagt tattgtgaat
     1681 ttttgagatg cccggcagaa ccaatagcac aacgatcaaa aggcaggcaa tcaaaactgg
     1741 tattgaggct tttagaatat gttttctcac aaatcgtctc ccaaagagtg cgtcgcctat
     1801 tatatctcca aaatcaacaa tttctgtctt atacgtgcag aaagcaggct cagacgggct
     1861 tgcacaatct cgcaaagcgg ggattggggt ctatgggtgg ggaatgcccc cacccataga
     1921 ccccaatttt cccctccagt ggtgggaaat cgacctactg cgattgctct atgcagaagg
     1981 tataatacgc tcatatccca caatgagtga gtatggagga tagtcgatgc aaacccattg
     2041 gcccctctcc ccttgggtcg aggaacaccg ccagcatatc ggcttcggcc tgcaggtgtt
     2101 cccgcgcggg gtcagcaccg aaagaaccca gcaattacat gcccttggac agctggctga
     2161 agggctgggt tacgacacct ttctcatcgg cgaccacccc gcccggggac ccgaaccatg
     2221 gttgcacctg gctgccctgg caatggctac ccagcgtatc cgccttggtt cgctggtcag
     2281 ctgcgccctc taccgccacc cgctgatgac cgccagactg ttatctgacc tggatcacct
     2341 gagcgcaggg cgcgctatcc tgggtttggg catcggctgg gacgctaacg agttcgccag
     2401 cctgggtatc cccttcctgc cggtgccgga gcggcaagca gccctggaag aggcgctggt
     2461 gatcatccgg ggggcgtgtg gagagagcca cttcacctat caggggcgct atttccagac
     2521 cgagcaggcg caggtgtcgc ccccgccact ccagagcccg ttccctccga tcttgatcgc
//
LOCUS       DBNHOH010000842         2963 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_887322, whole genome shotgun sequence.
ACCESSION   DBNHOH010000842 DBNHOH010000000
VERSION     DBNHOH010000842.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_887322"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..902)
                     /locus_tag="AC16V7_15170"
     CDS             complement(147..902)
                     /locus_tag="AC16V7_15170"
                     /inference="COORDINATES: protein motif:HMM:NF022441.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HIR6258511.1"
                     /translation="MYTKQSFPIKLLVMSMVIVVLIASCTNVLGFNRRVSGSGNVVQE
                     NRTLQSFTEVVLSEEGDLFIDVGDREQLIIEAEDNLQEYLVAEVNIGILEIKKLPENV
                     TLNATQPIRYYLTVKELKSLTIKNSGDAEVSEVRADKFLVHVTSSGSVHIGKLEVQRL
                     DVELTSSGDLVINEGEADEQFIRLSSSGEYIGKNVICQSAQVRLSSSGNATLNVLEVL
                     EADLSSSGNVYYIGNPRIVYKDSSSSGRVQPLP"
     gene            complement(1788..2378)
                     /locus_tag="AC16V7_15175"
     CDS             complement(1788..2378)
                     /locus_tag="AC16V7_15175"
                     /inference="COORDINATES: protein motif:HMM:NF021221.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnetosome magnetite crystal formation protein
                     family"
                     /protein_id="HIR6258512.1"
                     /translation="MNQPDLGLKVAELRQQKALTQEQLAEKCEVSTRTIQRIERGEVD
                     PRAYTLQCLGEALDFDFGEDTSQSESFWLVVMHLSSILPTLIIPLLIWSWKKKQSYKI
                     NEQGRDVLNFQITMMLLLFLGGFLLTFLPVALPSLGKALDDMGVNMVLFQFMVLCATA
                     PMVLIGIFCTYQGVVNAMRALLDKPVRYQLSLPFVK"
     gene            complement(2777..>2963)
                     /locus_tag="AC16V7_15180"
     CDS             complement(2777..>2963)
                     /locus_tag="AC16V7_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258513.1"
                     /translation="SGKSNVVRLDLPELSAEILVESEAPSFGGVEWEDPNELRLFDEN
                     GVEWGYDFKTEELEVVK"
BASE COUNT          815 a          762 c          601 g          785 t
ORIGIN      
        1 caggtaaaga gcataggtat ttcgctttag agagcgggcc catccccgcc aggcgttgaa
       61 catgttgatt tgccagcgga aatctatctc aagcgcggag gtcgatgcat ccagcaaacc
      121 attctcgccc aggtctgctc tagtacctac ggcaagggtt gaaccctgcc tgaagatgaa
      181 gaatccttgt agacaattct tggattacca atatagtaca cattcccgct actggatagg
      241 tctgcttcca gcacttccag cacattgaga gtagcattcc cgctactgct caatcttacc
      301 tgagcgcttt ggcagattac gtttttacca atatactcac cagagctgct taatctgata
      361 aattgttcat ctgcctctcc ctcattgatg accaaatctc cggaactagt caattccaca
      421 tccagacgtt ggacctccag cttgccgatg tgtacgcttc cagaacttgt cacatgaaca
      481 aggaatttat cggctctcac ttcagagacc tcagcatcac cgctgttctt tatggttaaa
      541 gacttgagtt cttttaccgt gaggtagtag cgtatgggtt gcgtcgcatt caaggtgacg
      601 ttttcaggaa gctttttgat ctctaagatg cctatattta cctcggcgac caggtattct
      661 tgcaaattat cttctgcttc aataattaac tgctcccggt ctccgacatc gatgaacaga
      721 tctccttctt ctgagagaac cacttctgta aagctctgca gcgttctatt ctcttgaacc
      781 acatttcctg aaccagagac tctgcgatta aaccccaata catttgtaca actcgcaatt
      841 agaacgacaa taaccatgct catgacgagc aatttgattg ggaacgattg ttttgtatac
      901 atgattatcc tttctagctc ttgacagctc ccctcaaact gtcaagggag caaaatcgaa
      961 tgcagaagat cattcataaa aaatccaaaa tcaaatgcca gtatgaccaa tcccacattg
     1021 agagccaaat ggcaaatccg tggccaattg gtcatgttaa cgtgcccgtc caaccacctg
     1081 aatatcactg ctaggatgcc aaacagagaa aatgtaaaag gaagggggaa atcccagcgc
     1141 cagaacaaca agctaatgac aaggttatgt aatagaccgt tcaccaaaaa aacagccagt
     1201 gttgcggtat tacgtctttt tttcccgcca aactttaagt acagtctata ggtgaaataa
     1261 ccgatacccg gattccatat ctgccagaag cgatgaaagc cgggctccag ccagcactcg
     1321 agaaaccctc gcctcacgaa aacccagtca tttacatgat ttccaaagcc acgcagtttc
     1381 aagtattgct tgtaattcaa aacatattgg ttataagagc gaatcactct tccttttatg
     1441 gcccaaatat cataggaacc cgacaggcta ctcttcatta ttctccaatt gcatagatat
     1501 tttggtattc gtcatctttg caaatattga agtgacgagt ccaatcgcct gtagaggcgc
     1561 ggtaaatact cgtggaatga tccgtagatc gaggccaacc ggagaatcaa cgacctttat
     1621 gttatctcgg gttgtaatct ccacatggcg gatagttcgg tcccttgcac ccttaataga
     1681 tctccagatg cctttagaag gaaattcgat gaggtttttg ctagacatat ttctcctcca
     1741 tgttactttg gctttactat ttcatgcagc aagactcgct acaaatatca tttgacgaat
     1801 ggaaggctaa gctggtagcg gactggctta tctaacagcg cgcgcatggc gttgaccacg
     1861 ccctggtaag tacagaatat tccgatcaat accattggcg cggtggcgca caacaccata
     1921 aattggaaca gcaccatatt cacccccatg tcgtccagtg cttttcccaa ggatggaagc
     1981 gcaacaggta aaaacgtgag caggaagccg cccagaaaca acaatagcat catggtgatc
     2041 tgaaaattga gcacatcacg cccctgttcg ttaattttat agctctgttt tttcttccag
     2101 gaccatatga gcagtgggat gatgagggtc ggcaggatgc tactcaggtg catgaccaca
     2161 agccaaaacg attcgctttg ggatgtatcc tccccaaaat caaaatcgag cgcctcgccc
     2221 aagcactgta gggtgtaagc gcgtggatcc acttcgccac gctcaatgcg ctggatggtg
     2281 cgcgtactga cttcacattt ttcagccagc tgctcctggg tgagtgcctt ttgctggcgt
     2341 agttcagcta ctttgagtcc aagatctggt tggttcatgg cgtactcctt tcgctatctc
     2401 cccattatta tagagaaagt acccaaaatc acaatgtcgt aaccacgaca ttcgcacgac
     2461 atttgctgtt tgaagaccga aagctcaact tgcatgcaaa tcagaataga agctttctcc
     2521 ttattctttt cacatattca tcttcactat tcgcacaaat gcaacagcac aaacctacat
     2581 ccgtattgta tctggatacc aggcaatcgc tccatgaagg ggtggaaccc cagataacgc
     2641 cagcaccatt tcgaacgtgg agattaggtt tgctgctctg tgtgaagacg acctatgctc
     2701 aaggcaaagt gtctatcccc gccatgcgat gaaaatgagg atttccccat agaaatcttt
     2761 ctcaggatta gaggtcttat ttcacgacct ccaattcttc cgtcttaaaa tcatatcccc
     2821 attccacccc attctcatcg aacaagcgca actcgttcgg atcctcccac tcgacacccc
     2881 cgaagctggg cgcttcggat tccaccagaa tttccgctga tagctcaggc aaatccagcc
     2941 gaaccacatt agatttgcca gaa
//
LOCUS       DBNHOH010000843         3981 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_117550, whole genome shotgun sequence.
ACCESSION   DBNHOH010000843 DBNHOH010000000
VERSION     DBNHOH010000843.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_117550"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="AC16V7_15185"
     CDS             complement(<1..1010)
                     /locus_tag="AC16V7_15185"
                     /inference="COORDINATES: protein motif:HMM:NF018012.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR6258514.1"
                     /translation="MLSLQSALCEKDIHRIHEHSLDVLMEVGIAYNTPRALNILEERG
                     CQVDYSRNWACIPPDLVEWAISQAPRKVTLAARDPSRDVLLDGLKSHHTTDSQGTQAI
                     DLQTGILHDSNAEDLRNAALFADALNMIEIVNVSVSASDIPAHLRTLFHFSQVFTQTS
                     KHVRTGVLNAEQVPFLVEMAKIVTGSDAFRPIFSVIDCTISPLMHDGLMTEACIDLAK
                     LQVPILVYPMPLAGGTSPVTLGGTMLMHNVEFLSGLVLYQCVNPGTPVIYGTGSSQLD
                     MHTGRYGGSADGFGMGLSLINLATFYDLPANLWGLSTKSKELGTQYGYEATAGTMLSY
                     LAG"
     gene            complement(1200..2132)
                     /locus_tag="AC16V7_15190"
     CDS             complement(1200..2132)
                     /locus_tag="AC16V7_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019587660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6258515.1"
                     /translation="MANVRDKKVKAQNSLSRHESMAAMIWRRFRHHPGAVVGAIVLSI
                     LIIATLLVPLSPYDPELSNVSMRLQPPSWQHPFGTDGLGRDMLTRVLYGGRISLLVGF
                     MVVVITLVIGVPIGAIAGYFGGWVDNILMRIIDATLALPSLLVLILLSAVLREVDHPL
                     IEKNNVLTIAVVIGLLSWMTVARLVRAIFLTLREIEYVTAAQALGASSLQIMASEILP
                     NGLGPIIVESTLEIGWAIMEESGLSFLGFGIQPPTASWGNLLGNAQEHLTTQPWLAIF
                     PGIMIFLTIISVNYIGDGLRDALDPYKILGQIGE"
     gene            complement(2241..3281)
                     /locus_tag="AC16V7_15195"
     CDS             complement(2241..3281)
                     /locus_tag="AC16V7_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6258516.1"
                     /translation="MGKYIVRRLLQAIPILFLISVILFTLVNIAPGGPLGGHGRTRRV
                     KQEKIDALKRQFGLDKPLPLQYVIWLIGNDWMQVDEDGDGILDSYGLRKGILRGDFGI
                     SIKTRNPVLEEIGDRLPNTIYLMTVTLLVVAIVAIPIGIISALRQYSAFDITATTLSF
                     MGQAIPEFWLGLILILIFYAWLDNPFTGEPLLPSGGMQTIGMEFSLWDRIQHLILPVT
                     MGMVGWIAWYSRFLRSSMLEVINKDYIRTARAKGQSERKVLYKHALKNALIPLVTIFA
                     LDIPYIFSGSLYVELLFSWPGMGRLYYDAATFRDYPVLLAVLIIGASLIILSNLLADI
                     AYAFLDPRVRFE"
     gene            complement(3375..>3981)
                     /locus_tag="AC16V7_15200"
     CDS             complement(3375..>3981)
                     /locus_tag="AC16V7_15200"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6258517.1"
                     /translation="DLLEEAGWTDSDGDGVRECRGCETAEEGTPMAMELAIYAEYGEE
                     LELAQQLVAEDLKAVGFDVTLQIVEGAVLWDPESGTEIRGNFDMDMWDDGYPGIDPTD
                     NQLWYYYHTDASDPESGGWNVMRWQNEDFDALYDELFTLDEEYREEVFCQIATLLDEE
                     VPQILLFSTLEMHGLSGHLNGVLPSVNDTVTWNIADWTLSE"
BASE COUNT         1067 a         1078 c          881 g          955 t
ORIGIN      
        1 cctgctaaat atgacagcat ggtgcctgcg gttgcctcgt agccatattg agtaccaagt
       61 tcttttgact tagtagaaag cccccacaga tttgctggca gatcatagaa cgtggcaaga
      121 tttatcagcg aaagtcccat tccgaaccca tccgcactgc caccatacct gcctgtgtgc
      181 atatccaatt gcgacgagcc tgtaccatag atcaccggtg tgccagggtt tacacattgg
      241 tacagcacca acccgctgag gaactccacg ttgtgcatca gcatcgtacc ccccaaggtc
      301 actggggaag taccccctgc cagcggcata gggtaaacca aaatgggaac ttgcagtttg
      361 gctaaatcga tgcatgcctc ggtcatcagt ccatcgtgca tcaagggcga gatggtgcaa
      421 tcaatcactg agaatatcgg gcggaaggca tcgctgccgg ttacaatctt cgccatttcc
      481 accaggaaag gcacttgttc ggcgttcaac acgccagtgc gcacgtgttt gctggtttgt
      541 gtaaatactt gagaaaaatg aaaaagcgtg cgcaagtggg ccggaatatc gctggctgaa
      601 acagagacat ttacaatctc gatcatgttc agagcgtcgg caaaaagcgc tgcgttgcgc
      661 agatcttctg cattggaatc atgaagtatt ccggtttgca ggtcaatcgc ttgagtgcct
      721 tgactatcgg tagtgtggtg agatttcaaa ccgtccagca gcacatccct ggatgggtca
      781 cgcgccgcga gtgtcacttt gcgaggtgct tgggaaatcg cccattcgac caggtcagga
      841 ggaatgcacg cccaattgcg gctgtaatcc acctgacagc ccctttcctc caagatattc
      901 agtgcacggg gcgtattgta tgcaatcccc acctccatca acacatccag gctgtgttcg
      961 tggatccgat gaatgtcttt ctcacataac gccgactgca aagataacat ttgaaatctc
     1021 tccaattacc cttcctcgat cagctcttat tggcacaagc caggctacga gctatctcct
     1081 tacgtttacc accttcttgt gacccgatct tatagcatcc acttaccaaa caatccgctc
     1141 acgcactacc tcaactggat ggcgatattg tccatccgcc ttcctttctg cttcctttct
     1201 cattcaccaa tttgtcctaa tatcttgtag ggatctaaag catcccgcag gccatcgcca
     1261 atgtagttca cagagataat agttagaaag atcattatcc cggggaaaat tgccagccaa
     1321 ggttgggtcg tgagatgttc ttgagcattg ccaagcaaat ttccccaact tgctgttggg
     1381 ggttggatgc caaatcccaa aaaagaaagc ccagactctt ccatgatcgc ccaaccaatc
     1441 tcgagcgtcg attcaacaat aatagggcct aagccatttg gcagaatctc acttgccata
     1501 atctgcaagc tagatgcgcc tagggcttgc gctgcagtga catattcgat ttcccgcagg
     1561 gtgagaaaga tcgcccgtac tagacgcgcc acagtcatcc acgagagcaa gccaatcacc
     1621 acggcaatgg tcaacacgtt attcttttcg attagcggat gatcaacttc cctcaatact
     1681 gcgctaagca agataagcac caacaaagaa ggcaaagcca acgttgcatc aataattcgc
     1741 atcaagatat tgtctaccca gcctccgaag tacccagcaa tcgctcctat ggggacaccg
     1801 attactaacg tgatgacaac caccataaat ccaaccagca atgatatgcg accaccatac
     1861 agcacgcgcg tcagcatatc cctgcccaaa ccatccgtcc caaaaggatg ctgccacgag
     1921 ggcggttgta agcgcattga gacattggac agctcaggat cgtatggaga taacggtact
     1981 agaagcgtcg caatgattaa tatggaaagt acaatagccc caacgactgc gccaggatgg
     2041 tgacgaaacc ttcgccagat catggcagcc atgctttcat gccttgacaa gctattctga
     2101 gctttcacct ttttgtctct tacgttggcc atgttactca tcacaggata aatattgttt
     2161 ctttttgatt tgccttgttc taggcgagtt gtgggaggtc atccattttt aaaccgtcca
     2221 gcagcttcct aaaaaagaaa tcactcaaag cgaacccttg ggtctaaaaa ggcataggca
     2281 atatctgcca acaagttgct caagataatt agactcgcac cgataattaa aaccgccagc
     2341 aacaccggat aatcgcggaa agtagccgca tcatagtaaa gacgccccat gcctggccat
     2401 gagaaaagca attccacgta cagtgaacca gaaaaaatat aagggatatc caaggcgaaa
     2461 atcgtcacca gggggattag ggcgtttttt aacgcatgct tgtacaatac ctttcgctct
     2521 gattgtcctt tggcgcgcgc tgtccgaata taatctttgt tgatcacctc gagcatactc
     2581 gagcgcaaga accgcgagta ccatgctatc caaccgacca tgcccatcgt caccggcaag
     2641 atcaaatgct gaattcgatc ccatagggaa aattccatac cgatggtttg cataccacct
     2701 gacggtaaaa gtggctctcc agtaaaggga ttatctaacc aggcgtaaaa gatcagaatc
     2761 aagatcaggc ccagccaaaa ctccggaatc gcctgcccca taaaagacaa tgtggtagca
     2821 gtaatatcaa acgctgagta ttgcctgagg gcggaaatga tcccaatagg aatcgccaca
     2881 atggctacta caagtagggt gacggtcatc aagtaaatgg tattgggtaa tcggtctcca
     2941 atctcttcta acacaggatt gcgcgttttt atcgagatcc caaaatctcc tctcaagatg
     3001 ccctttctta agccatagct gtccaagatt ccatccccat cttcgtccac ctgcatccaa
     3061 tcgttaccaa tcaaccaaat cacatattga agaggcaatg gtttatccag gccaaattgt
     3121 cgcttaagcg catcaatctt ttcctgcttg acccgtcggg tgcgcccatg tcctcccagg
     3181 ggcccccctg gggcgatgtt cacgagggta aacagaatga cactgatgag aaataatatt
     3241 ggaattgcct gaagtaggcg acgaacgatg tacttgccca tctacactcc ttcagtcctt
     3301 tcaagtattg gtagggagaa gggatgtgcc cttctcccta tgatagatgg tgttgccaaa
     3361 atcacccttg agggttattc gctgagcgtc cagtctgcta tgttccaagt caccgtgtcg
     3421 ttgacggaag gcaacacacc gtttaggtgc ccagaaagcc catgcatctc cagcgtggag
     3481 aacagcagga tttgggggac ctcttcatcg agaagtgtgg ctatctgaca gaaaacctct
     3541 tcgcgatatt cttcgtcgag agtaaacagc tcatcataca atgcgtcgaa atcctcattt
     3601 tgccagcgca tgacattcca gccaccgctc tcaggatctg aggcatctgt atggtagtag
     3661 taccacaact ggttgtctgt ggggtcaatg cccggatatc catcgtccca catatccatg
     3721 tcgaaattgc ctcggatctc ggtacccgac tcgggatccc acagaactgc accctcaacg
     3781 atctgtaaag tcacatcaaa accaacggct ttgaggtcct ctgcgaccaa ctgctgagcc
     3841 aattctagct cttcgccata ctcagcatat atcgctagct ccatcgccat cggggtgcct
     3901 tcttctgcgg tctcgcagcc acggcattca cgaacaccgt cgccatcaga gtctgtccac
     3961 ccggcttctt caagcaaatc t
//
LOCUS       DBNHOH010000844         2181 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1143131, whole genome shotgun sequence.
ACCESSION   DBNHOH010000844 DBNHOH010000000
VERSION     DBNHOH010000844.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1143131"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="AC16V7_15205"
     CDS             complement(<1..742)
                     /locus_tag="AC16V7_15205"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6258518.1"
                     /translation="MMSISHIWAVTRKEFLHIIRDRSTLFLVLFTPTLVLLLMAYALT
                     VDIKHVPIGILDYDRSPTSRVFIQQITAGQDLDIFAYAETMDEIDSMLMHGKIKAALV
                     IAPGFSSELQGLKGMPIQVIIDGTEPESGNFAMQHITRRAEEYLDQVLTRRLQAAGVS
                     LASLQPLDLRVHTWYNPDLKPRNDLIPGLISMVLGFPALSVALTLAHEREHGTLEQLL
                     ATPITRGELLLGKIVPYLAVGLVNVVMIP"
     gene            complement(739..1677)
                     /locus_tag="AC16V7_15210"
     CDS             complement(739..1677)
                     /locus_tag="AC16V7_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6258519.1"
                     /translation="MEMIVVPVIETHELARNFKATRAVDGLDLSVQPGELFGLVGPDG
                     AGKTTTLRLLAGLLDVSGGEATILGFDLKSQPEAIKPRVGYMAQQFSLYAELSVLENM
                     RFFAELFDVSARDLSERMQGLLAFAGLTEFKDRRAAHLSGGMQKKLALACTLVHRPEV
                     LLLDEPTTGVDPISRREFWNILTELHLDGTTIMVSTPYMDEAERCSRVGLMFAGKLVV
                     CDTPQNIRNRLEGDVIELLSEDWQSARERVAGLPGVREVQTYGESLHLVVDAGEKRLP
                     EIESALKKAGVDLRSMRIAPPRMEEAFISLIRKMEK"
     gene            complement(1678..1950)
                     /locus_tag="AC16V7_15215"
     CDS             complement(1678..1950)
                     /locus_tag="AC16V7_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258520.1"
                     /translation="MLQVIILVLAMVLVGLLVGWLAGLIWKNQRPIGVSGDYMVAIIS
                     AIVIGLIDWFVIPAMGFSDTMKYLGVAFEPLLGALLVLWIIRKAKQ"
BASE COUNT          501 a          652 c          570 g          458 t
ORIGIN      
        1 cggggatcat cactacattc accagaccta ccgcgagata cgggacgatc ttccccagca
       61 gcagttcgcc gcgcgtgata ggcgtggcca ggagttgctc cagcgtcccg tgctcgcgtt
      121 cgtgagctag cgtcagcgcc acagataggg ctgggaagcc caacaccatt gagatcaggc
      181 cagggatcag gtcattgcgg ggtttaagat cggggttgta ccaggtatgc acgcgcaagt
      241 ctagtggttg caacgaggcg agcgagacgc ccgcggcctg caaacggcgt gtcaatacct
      301 ggtcaagata ttcttccgcc cgccgggtga tgtgctgcat ggcaaagttg ccgctttccg
      361 gctctgtccc atcaataatg acctgtatcg gcatcccttt gagcccctga agttcactgg
      421 aaaatcccgg agcgatgacc agggcagctt taatttttcc gtgcatcaac atggaatcga
      481 tctcatccat ggtttctgca taagcaaaga tatccaaatc ctgcccggcg gttatctgct
      541 ggataaaaac gcgcgaagtt gggctgcgat cgtagtccag tatgccaatc ggcacgtgct
      601 taatatctac agtcagggca taggccatca gcaggagcac cagggtgggt gtaaaaagca
      661 ccaggaagag cgtgctccgg tcgcggatga tgtggagaaa ttccttgcgg gtcaccgccc
      721 agatgtgaga aatcgacatc atttctccat tttgcggatc agcgaaataa acgcctcttc
      781 catgcgaggt ggagcgatgc gcatgctccg caagtcaacg ccggccttct tcaacgcgct
      841 ttcgatttcg ggcaggcgtt tttctcccgc atcgaccacc agatgcaacg actcgccgta
      901 agtctgcacc tcacggacgc ccggcaaccc tgcgactctt tctcgcgcag attgccaatc
      961 ctcgctaagc agctcgataa cgtcgccctc cagccggttg cggatatttt ggggcgtatc
     1021 acacaccacg agcttgccag cgaacatcaa ccccacccgc gagcagcgct ccgcttcgtc
     1081 catatagggc gtgctgacca tgatggtggt tccatcaagg tgcaattcgg tcaaaatatt
     1141 ccaaaactcg cgccgcgata tggggtcaac gcccgtggtg ggttcgtcga gcagcaggac
     1201 ttccggccga tgaaccaggg tgcacgccag ggctagtttc ttctgcattc cacccgaaag
     1261 atgggctgcg cgccggtcct tgaattcggt caagccggca aaagccagca gcccttgcat
     1321 ccgttcggac aagtctcggg ctgagacatc aaaaagttca gcgaagaagc gcatgttttc
     1381 cagcacggaa agttcggcgt acaggctgaa ctgctgcgcc atataaccaa cccgtggttt
     1441 gatggcctcc ggctggctct tgagatcaaa accaaggatg gtggcctcgc cgccgctcac
     1501 gtccagcagc cccgccagca ggcgtaaggt agtggtcttc cccgccccgt ccggccctac
     1561 caatccaaaa agttcccctg gctgtacgga taaatccaac ccatccaccg cgcgggtggc
     1621 tttaaagttt cgtgcgagtt cgtgggtctc gataaccgga accacgatca tctccattta
     1681 ctgcttggct ttgcggatga tccaaagcac gagcagcgcg cccagcaaag gttcgaaagc
     1741 cacgcccaaa tatttcatcg tatcgctgaa gcccatagct gggatcacaa accagtcaat
     1801 caatccgatg acaatagccg agatgatagc caccatgtaa tccccgctta caccgatcgg
     1861 acgctgattt ttccagatta ggccagccag ccagccaacc aacaggccga caaggaccat
     1921 tgccaggact aagataatta cttgcagcat gtctcaaact cctttatttg tagaagctac
     1981 gctgttgggg catgggtagc gagggctaag cccttatccc ctcgagcaac agcctaatcc
     2041 cggagctgat atggcgttca ggctccacca tattttgatc gtatacccat aatagaatcg
     2101 tcccttcgaa gatcgcccca atcgctatgg cgacctcgcg cgcctccacc gtgcgaaact
     2161 cgcccgagtc aatgccgccc t
//
LOCUS       DBNHOH010000845         4412 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_695254, whole genome shotgun sequence.
ACCESSION   DBNHOH010000845 DBNHOH010000000
VERSION     DBNHOH010000845.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_695254"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="AC16V7_15220"
     CDS             <1..834
                     /locus_tag="AC16V7_15220"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR6258521.1"
                     /translation="AHLTDIHVQPEGIAPDGMVKALRHAQEQPDPPDLIINTGDSIMD
                     SLNTDKARTQAQWEVFNSILTAECKLPIIQAIGNHDVWGWAHPDQSIRSDPLYGKNMA
                     IEKLGLSHRYYSFDQAGWHFIVLDSNHPPNEVSGYPYIGKLDEEQFAWLVQDVESTPH
                     EMPICILSHIPIVAACEYFDGPNEESGNWVVPAAWMHIDARRFRQFFLQHPNIRLCLS
                     GHTHQLEALDYLGVRYLTNGAVCGNWWNGTYLDFPPAYVMVDLYQDGSFESISVHYEN
                     V"
     gene            complement(897..1550)
                     /locus_tag="AC16V7_15225"
     CDS             complement(897..1550)
                     /locus_tag="AC16V7_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIR6258522.1"
                     /translation="MELITYLIDLFLHLDKHLSIVIQNYGAWTYALLFLIIFLETGLV
                     ITPFLPGDSLLFAAGTFAGLGSLNVIVLFLLLSAAAIIGDTVNYWIGHYIGPRAFSGE
                     IRFLKKEYLDRTHEFYEKHGGKTIILARFVPIIRTFAPFVAGIGAMSYGRFITYNVVG
                     GVVWVAIFTFGGHFFGNLKFVQDNFTFVILAIIFISVLPGVFEFLKERSRSAQKVEA"
     gene            complement(1682..2938)
                     /locus_tag="AC16V7_15230"
     CDS             complement(1682..2938)
                     /locus_tag="AC16V7_15230"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HIR6258523.1"
                     /translation="MDNSVYNQPTNINQAHDIHWPSVLQLGFSLLGISILWSGAFILV
                     LIGLIQLLLPAEAQGNSTIPLLLGSSGVMIGLLLVPSVVYPLQRLLGHRPSRLVPTSP
                     WLRPTLLIFALPLLLLAGWWISQQGAALQLILPIFHVPAIGIPILWLSYLAVRDLPLG
                     SPQRAWGLLGSGSVLAPLFILFAEVLVLLAGVITWSIWMSGQPGMLDKLSILAERLRQ
                     AGNSPQLIAHIISPYLKDPLVIFSAFTFAAVIVPLIEEAIKPLGVWFLAGSRLTPAEG
                     FTAGILCGAGYALVESLTLSADSGNDWVSLVFARIGTAVVHILTSGLTGWALASAWRE
                     KRYFRLGVTYLGAVLLHGLWNGLTMLIVIADLSQLNDQLGQHLLSQAYQIVPYGLVGL
                     AIAGLATLLWINVRLRRTTDSPQAVV"
     gene            complement(2975..3535)
                     /locus_tag="AC16V7_15235"
     CDS             complement(2975..3535)
                     /locus_tag="AC16V7_15235"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR6258524.1"
                     /translation="MKQGNIAAVIRLELMESRQFSECPPMTIDASKQYVAILHTEKGD
                     ITIELYADKAPLAVNNFIFLARNGWYDEVTFHRVMENFVAQAGDPSGTGFGTPGYAFS
                     NEISPDLKFDKAGIVAMANAGPDSNGSQFFITYAPTPQLDGGYTIFGQVISGMDVAES
                     LTPRDPSQALDLPPGDEIISVEIQER"
     gene            complement(3560..3931)
                     /locus_tag="AC16V7_15240"
                     /pseudo
     CDS             complement(3560..3931)
                     /locus_tag="AC16V7_15240"
                     /inference="COORDINATES: protein motif:HMM:NF024853.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
     gene            complement(3944..>4412)
                     /locus_tag="AC16V7_15245"
     CDS             complement(3944..>4412)
                     /locus_tag="AC16V7_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258525.1"
                     /translation="CLVGTNLQSSGIPHPACRFPPQSMVEGSGFLKIFSRIGYNGQIE
                     IHTTGSRLPMKKIILLLTGMALTLAACQGQAVSPQPAPTEAATPSLVPTIQATLAEAT
                     PTQPEAPAGCTAISPKPTPGPTETSLFPPVSPDDWVKGPADASITLTEYSDFQ"
BASE COUNT         1060 a         1152 c         1247 g          953 t
ORIGIN      
        1 gctcacctga cggacattca cgttcagccc gaagggatcg ctcccgacgg catggttaaa
       61 gcccttcgcc atgcacagga gcaacctgac ccacccgatc tcattattaa cacgggcgat
      121 tcgatcatgg attcacttaa tacggataaa gcccgcaccc aggcacagtg ggaggtattc
      181 aatagtattt tgactgctga gtgtaaactc cccatcatcc aggccattgg aaaccacgat
      241 gtatgggggt gggctcatcc agatcaaagc attcgaagtg acccgctata cggtaagaat
      301 atggcgatcg agaaactggg cctctctcac cgctactaca gtttcgacca agctgggtgg
      361 cactttattg ttctggacag caatcacccg cccaatgagg tgtctggata tccatatatt
      421 ggcaaattag acgaggagca attcgcgtgg cttgttcaag atgtcgagtc gacgccccac
      481 gaaatgccaa tctgcatcct ttcacacatc cccattgtgg ctgcatgcga atacttcgat
      541 ggccccaacg aggagagcgg caactgggtt gtgcccgcag cctggatgca cattgatgcg
      601 cgtcggttca ggcagttttt cttgcagcac ccaaacatcc ggctgtgctt gagcggccac
      661 acccatcagc ttgaagcgct cgattacctg ggcgtacgct atctgacaaa tggggcggta
      721 tgcgggaatt ggtggaacgg cacctatttg gatttccccc cggcttatgt catggtggat
      781 ctgtaccagg atggttcttt cgaaagcata tctgtccatt atgaaaatgt ataaaaaaag
      841 cgacgctgtg tgcagcgccg ctaatagaga tcgtggatgg ggggaggtta gggcttttac
      901 gcctctacct tctgggcaga gcgggagcgc tctttgagga attcaaatac tcctggtaag
      961 accgagatga agataatcgc caggatgacg aaggtgaaat tatcctggac gaatttcagg
     1021 ttgccgaaga agtgaccgcc aaaggtaaat atggctaccc agaccacgcc gcccaccaca
     1081 ttataggtga tgaagcgccc gtaggacata gccccgatgc ctgccacgaa gggtgcaaag
     1141 gtacggatga tgggcacaaa tcgcgccagg atgatggtct tgccaccatg cttctcgtag
     1201 aactcgtggg tgcggtccag atattctttc tttaggaagc gaatctcccc gctgaaagcc
     1261 cgtggaccga tgtagtgtcc aatccaatag ttgactgtat cgccgataat cgccgcagcg
     1321 gagagcagca gaaacaacac gatcacatta agcgatccaa gcccggcgaa agtgcctgcg
     1381 gcgaagagca gggagtcgcc tggcaggaag ggggttatta ccagtcctgt ctccaggaag
     1441 atgattaaga aaagcagcgc gtaggtccag gcgccgtaat tctggataac aatactcagg
     1501 tgcttgtcca aatgaagaaa taaatcgatc aaatacgtaa ttaattccat ttcaatcctt
     1561 tcatctcgct ttccccctga gggagttcag cttgctgaag gccataggcc tcccaatttc
     1621 aggcttgctc ccttttccag tacaggtggg gtgagagtta tcttgatcaa agtcccgccg
     1681 attatactac agcttgagga gaatctgttg tgcgtcgcag gcgcacatta atccacagca
     1741 aggtcgctaa cccggcgatt gccagaccaa ccagcccgta ggggacgatt tggtaagcct
     1801 gggaaagcag atgctgccca agctggtcgt tgagttggga aagatctgcg atcacaatca
     1861 gcatggtcag gccattccac aagccgtgta gcaagacagc ccccaagtag gtgacaccca
     1921 ggcgaaagta tcgcttttct ctccaggcag atgccagtgc ccagccggtc agcccgctgg
     1981 tgaggatgtg cactacagcg gttccgatgc gggcgaaaac cagcgagacc caatcgttac
     2041 cactatcggc agacagggtt agactctcca ccagtgcata gcccgctccg cacaagatgc
     2101 ctgcggtaaa cccttcggct ggcgttagcc tcgagcccgc caggaaccat acccccaatg
     2161 gcttgatggc ctcttcgatc aagggtacga tgaccgcggc aaaggtaaag gctgaaaata
     2221 tcaccagcgg atctttgaga tagggggaaa tgatgtgggc gatgagctgc ggcgagttgc
     2281 ccgcttgtcg caggcgctcg gcaaggatgg agagtttgtc cagcatgcca ggctgtcctg
     2341 acatccaaat cgaccaagtg attacaccag ccagcagaac cagtacttct gcgaaaagga
     2401 tgaatagcgg cgccagtacg ctgccgctcc ccaacaagcc ccaggcgcgc tggggagaac
     2461 cgagcggcag atcacgcacc gccagataac tcagccacaa gatcggtatg ccaattgccg
     2521 ggacatggaa gataggcagg atgagctgta atgccgcccc ctgttgggag atccaccagc
     2581 cagccaaaag gagcaggggc aaagcgaaga tcaacaaggt cggtcgcagc catggcgagg
     2641 ttggcactaa acgggaaggg cggtgtccca ataaacgctg caaagggtaa actacggagg
     2701 ggaccagcag cagtccgatc atcaccccag acgacccaag taatagaggg attgtgctgt
     2761 tgccttgcgc ctctgcaggc agcagcagtt gtatcaggcc gatcaacacc aggataaagg
     2821 ccccactcca caggatggat atgccaagca ggctgaagcc tagctgcagc actgaaggcc
     2881 agtgaatatc gtgtgcctgg ttgatgttgg ttggctggtt atagaccgag ttatccaaaa
     2941 atatatccct aaatttgcga cattttgctt gtagctatct ctcttggatt tccacgctga
     3001 tgatctcgtc gccagggggc agatccagcg cctgggaggg atcgcgtggt gttaggcttt
     3061 cagctacgtc catgccagaa atgacctgtc caaagatggt atagccgcca tctagctgag
     3121 gggttggtgc ataggtgatg aaaaattggc tgccattcga gtcgggtcca gcgtttgcca
     3181 tcgccacaat cccggcttta tcgaacttga ggtcggggga tatctcattg gagaaggcat
     3241 atcctggggt gccaaagcct gtgccgctgg gatcgccagc ctgggctacg aagttctcca
     3301 tcacgcgatg gaaggtcacc tcatcatacc agccgttgcg agccaggaag atgaaattat
     3361 tcaccgccaa aggagcttta tctgcataca actcgatcgt aatgtcgccc ttctcagtgt
     3421 gcaggatggc gacatattgt ttggaggcat cgatggtcat cggcgggcac tcactgaatt
     3481 gccttgactc catcaattcc aggcggatca cggctgcaat gttcccctgt ttcatgggca
     3541 ggttgctagg ccagatgctt tgattgacca gcaagaacgg cgtgccgggc atgccgatct
     3601 ccacccctcg atcccaggca gcctgcgctt tgtcggcgag gggctggctg gtaagatcac
     3661 tggaaaactg ctccgtattg aggtccaact cttcggcacg ggcgatgacc cactcttgga
     3721 actcttcaac gctaagatct acccattccc cctggcgttc aaagagcaga tcgtgcatct
     3781 cccaaaattt accttgcatc ccggcagcct cggcagcttg agccgatagg gcggccttat
     3841 catggatgga gatcaacggg aaatggcgat agacgatgcg gatatctttg ggtgactctt
     3901 cttgtagctg cgccaacacg ggcgccagat cagcacagcc tggtcattgg aaatcgctat
     3961 actcggtcag cgtgatggag gcatcggcag ggcccttgac ccaatcatct gggctgacgg
     4021 gtgggaatag cgaggtttcc gtcgggccag gcgtgggctt ggggctgatc gcggtgcagc
     4081 cagccggggc ttctggctga gtgggtgtgg cttctgccag ggtggcctgg atggttggga
     4141 ccagggaggg cgtagctgct tcggtcggtg cgggctgagg cgaaactgcc tgcccctggc
     4201 aagcggcgag ggttagcgcc atgccggtta acaataggat gattttcttc ataggtagtc
     4261 ttgatccagt tgtgtgaatt tcgatctgcc cattgtatcc tattctggag aatattttta
     4321 aaaaaccact tccctccacc atagattgag gagggaagcg gcaagcggga tgggggatac
     4381 cagatgattg cagattcgtc cctaccagac ac
//
LOCUS       DBNHOH010000846         3430 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1465027, whole genome shotgun sequence.
ACCESSION   DBNHOH010000846 DBNHOH010000000
VERSION     DBNHOH010000846.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1465027"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2142
                     /locus_tag="AC16V7_15250"
     CDS             <1..2142
                     /locus_tag="AC16V7_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HIR6258526.1"
                     /translation="KDLLYTVEPYAHRYPTCWRCRSELVFRLVDEWFISMGEVYDKPR
                     QALTADEKARSLRYQIMDVVDQIRWIPEFGHSREMDWLRNMHDWMISKKRYWGLALPI
                     WVCDSCGHYHVIGDEVELKERAIDGWEKFEGHTPHRPFVDAVKLECPECKGVMSRIPD
                     VGNPWLDAGIVSFSTLRYRTDSDYWRKWYPAHWISESFPGQFRNWFYSLLAMATVIDS
                     SPPFLENFGYATLLAEDGRPMHKSWGNSIEFNEAADNMGVDVMRWLYCAHKPENDLLF
                     GYNRGDDVRRRFIIPLWNVYSFLATYARLDGWEPTLNSTGGESSFDTASPEGPTPHSE
                     NLLDRWIVARLNQVTARCTEALENSDTYSATLALEALLDDLSNWYVRRSRRRFWKSEH
                     DTDKEDAYTTLYHVLVKLIRILAPFIPFTTEAMYQNLVRAAWETSAVELPKGQSPYTS
                     VHHTAWPQADTATVDESLLDQMALARQVASLGLSARNAAGLKVRQPLAKVMVYAGRKR
                     TLRDELVEIVTDELNVKSLEFVQDPKRLVSYRILPDNKLLGPRFGARFPALRQAIAEA
                     DPYQVASSVSAGLPVSLQVDGESVELAPEEIVVNTQPAEGLAVAADKLITVAVDATIT
                     PELRLEGLAREVVRRVQAMRKDAGFDIADRITTYYAATGELAQVFDAWGGYIEAETLT
                     TNLISGDPPEEAFKEVHKVEGESITLGVKRN"
     gene            2197..2775
                     /locus_tag="AC16V7_15255"
     CDS             2197..2775
                     /locus_tag="AC16V7_15255"
                     /inference="COORDINATES: protein motif:HMM:NF013921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR6258527.1"
                     /translation="MARRKVDFASGQYYHIYNRGANRGLIFHVDDNYLFLLRRLNEHS
                     TKLGISVIAYCLMPNHFHFLLRQDGEFPISHLMQAVFNSYTKAFNKAFGRTGTLFEGP
                     FEATHVDRNDYLVHLCRYVHRNPLDAGLVSNPSQWPFSNYLEWIGKRNGSLVDQAFVH
                     DFFPDPRGYEIFVMEYTPSQEINSAIKRFSFD"
     gene            complement(2819..>3430)
                     /locus_tag="AC16V7_15260"
     CDS             complement(2819..>3430)
                     /locus_tag="AC16V7_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HIR6258528.1"
                     /translation="GYVFSIWGLIYLGLILFAVYQALPAQRDNPRLSQVGLLFVLSCL
                     ANIAWLFLWHYERFPLTLVAMFTLLISLIAIYLRLGIGRTAVSPGERWLVQIPFSVYL
                     GWVSVATIANVTDVLDYIKWNGWGIGPEVWAIIMLVVGLALTVAMSLTRGDFAYALVV
                     LWAYVGIAVKQAGTPLVAVSAWVAAGLVVVLIVITAVLRPRLG"
BASE COUNT          786 a          989 c          963 g          692 t
ORIGIN      
        1 aaggatctgc tttacactgt tgagccctac gcccaccgct accccacctg ctggcgctgc
       61 cgttccgagc tggttttccg cctggtagat gagtggttca tcagcatggg cgaggtatac
      121 gacaagccgc gccaggcctt aactgccgac gagaaagcgc gcagcctgcg ctaccagatt
      181 atggacgtgg tcgaccagat ccgctggata cctgaattcg gccactcgcg cgaaatggat
      241 tggctgcgca acatgcacga ctggatgatc agcaagaaac gctattgggg gctggcgttg
      301 cccatctggg tgtgtgatag ctgcgggcac taccatgtca ttggcgatga ggtggagctg
      361 aaagagcgtg ccatcgatgg ctgggagaag tttgaaggcc atacgccaca ccgtcctttc
      421 gtggacgcgg tcaagctgga atgccctgaa tgcaaagggg taatgagccg catccccgac
      481 gtgggcaacc cctggctgga cgccggcatc gtatccttca gcacgctgcg ctaccgcaca
      541 gattcagatt actggcgcaa gtggtatccg gcgcactgga ttagcgaatc cttcccaggt
      601 cagttccgca actggttcta cagcctgctg gctatggcaa cggtcatcga cagcagccca
      661 cccttcctgg agaacttcgg ttatgccacc ctgctggccg aggatgggcg cccgatgcac
      721 aaatcttggg gcaactccat cgagtttaac gaggctgccg acaatatggg tgtggatgtg
      781 atgcgctggc tgtactgtgc tcacaagccg gagaatgacc tgcttttcgg ctataaccgc
      841 ggtgacgacg tgcgccgccg tttcatcatc ccgttgtgga acgtgtacag cttcctggcc
      901 acctatgccc gcctggacgg ctgggagcca accctcaact caacgggagg tgagagcagc
      961 ttcgacaccg cctcgccgga aggtcccact ccacactccg aaaacctgct ggaccgctgg
     1021 attgtggcac ggctgaacca ggtgactgcc cgctgcaccg aagctttgga gaattcagac
     1081 acatacagtg ccaccctggc tctggaagcc ttgctagacg atttatccaa ctggtacgtg
     1141 cgccgcagcc ggcgccgctt ctggaaaagt gagcacgata cagacaaaga agatgcctac
     1201 actaccttgt accacgtgct ggtcaagctg atccgcatcc tggcgccctt tatcccgttc
     1261 acgaccgaag ccatgtacca gaacctggtg cgcgctgcat gggaaacctc agcagttgaa
     1321 ctgccgaagg gtcaatcccc ttacacaagc gtgcaccaca ccgcctggcc ccaggcagac
     1381 acagctaccg tggacgaatc tctgctcgac cagatggccc tggcgcgcca ggtagccagc
     1441 ctgggtctga gcgctcgtaa cgcggccggg ttgaaggtgc gccagccgct cgccaaagtg
     1501 atggtctacg ccggcagaaa gcgaacattg cgcgacgaac tggtggagat cgtcaccgat
     1561 gagctgaatg tcaagtcgtt ggagtttgtc caggacccaa agcggctggt gtcgtaccgc
     1621 atcctgccgg ataacaaact gctaggaccc cggtttgggg cgcgcttccc cgcgctgcgc
     1681 caggctatag ctgaggctga cccctaccag gtggcaagca gtgtgtccgc ggggctgccg
     1741 gtcagcctgc aggtggatgg agagtcggtt gagctagccc cggaggagat cgtggtgaac
     1801 acccagccag ccgaaggtct ggccgtagca gctgataagc tgatcactgt ggctgtagat
     1861 gctaccatca cgcccgagtt gcgcctggag ggtctggcgc gcgaagtggt gcggcgcgtt
     1921 caggccatgc gcaaagacgc tggctttgac atcgccgacc gtatcaccac ctattacgcg
     1981 gcgacaggcg agctagccca ggtcttcgac gcctggggag gctatatcga ggctgagacg
     2041 ctgaccacga acctgatctc aggcgaccca ccggaggagg cttttaaaga agttcacaag
     2101 gtggaagggg agtcaatcac ccttggtgtc aaacggaact gagtaacgtg cgacgcactt
     2161 cgaaagtgcg tcgcacgttg gtcttcaaga attaacatgg cgcgcagaaa agtcgacttt
     2221 gcgtctggtc agtattacca tatctacaac cgtggggcaa atcgaggatt gatctttcac
     2281 gtagacgaca attacttatt cttgcttcgc cgcctaaatg agcattcaac aaagcttgga
     2341 atcagtgtca tcgcctattg cctgatgcca aaccattttc actttctgct acgccaagat
     2401 ggcgagtttc cgattagcca tcttatgcaa gcggttttca atagctatac caaagcattc
     2461 aacaaagcat ttgggagaac tggtacacta tttgaagggc cttttgaggc gacccatgta
     2521 gatcgaaatg attatctcgt tcatctatgt cgttatgtcc atcgtaaccc acttgatgct
     2581 gggttagtct ctaatccaag tcagtggccg ttctcgaatt atctagaatg gattggtaag
     2641 cgtaatggct cgctggtaga tcaggctttc gttcatgatt tctttcctga tccaagaggc
     2701 tatgaaatat tcgttatgga atacacccca tctcaggaaa tcaacagtgc aattaagcgg
     2761 ttcagctttg attaggtgcg acgcacttta gaagtgcgtc gcacctggat cgcaggcatt
     2821 atcccagcct ggggcgcagc acggcagtga tcacaatcaa caccaccacc aatcctgctg
     2881 ctacccaggc ggagacggct accagcggtg tgccagcctg ctttacggca atccccacat
     2941 aagcccacag gaccacgagc gcataggcga aatcaccccg ggtgaggctc atggcgacag
     3001 tgagcgccag accaaccacc agcatgatga tcgcccacac ctccgggccg ataccccagc
     3061 cgttccactt tatataatcc agcacatcgg tcacgttggc gatggtcgcc acactcaccc
     3121 agcccaggta cacgctgaac gggatctgga cgagccagcg ctcgcctggg gaaacggctg
     3181 ttcgcccgat ccccagccgc aggtagatgg caatcagcga gatcaggagc gtgaacatag
     3241 ccaccagggt cagagggaag cgctcgtaat gccataagaa cagccaggcg atgttcgcca
     3301 ggcaacttaa gacgaagagc aaaccaacct ggctcagccg cgggttatcc ctctgcgcgg
     3361 ggagcgcctg gtagacggca aacaggatca accctaaata gatcagcccc cagatcgaga
     3421 agacatagcc
//
LOCUS       DBNHOH010000847         1972 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_565879, whole genome shotgun sequence.
ACCESSION   DBNHOH010000847 DBNHOH010000000
VERSION     DBNHOH010000847.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_565879"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="AC16V7_15265"
     CDS             complement(<1..218)
                     /locus_tag="AC16V7_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258529.1"
                     /translation="MMRQLYLSGDHFAMGKQHGHQVTDLKPHILSAIQHRLDKLAKVE
                     LNTGHLEEQMLESWHAQAEPLLEMMQGIS"
     gene            complement(237..1304)
                     /locus_tag="AC16V7_15270"
     CDS             complement(237..1304)
                     /locus_tag="AC16V7_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6258530.1"
                     /translation="MKEAQSESTPTPLFEGKGIYMHFQIRSSLLARILAGVKEKVVRA
                     VDGVDLAIWPGETLGLVGESGCGKTTLGRVLTRLYEPTRGQIYYRGQPVKGDTILEQQ
                     DGSNQTRRVNYHRLAQIIFQNPYSSLNPRKTVREIIGTPLQQRGVRDPLLREEETRQL
                     LQRVGLSKRHIDRYPHQFSGGQRQRIGIARSLAMRPKFIVADEPVSSLDVSIQAQVIN
                     LLEELQAEYHLTYLFIAHDLSVIYYISDRVAVMYLGHIVELGKTDYLFESPLHPYTQA
                     LLAAIPRVSKAARKERILLPGGVPSPIDPPSGCPFHPRCSAKVGPICEQEYPPFFQVN
                     NQQVACWLYQDHSIADQSSTK"
     gene            complement(1301..>1972)
                     /locus_tag="AC16V7_15275"
     CDS             complement(1301..>1972)
                     /locus_tag="AC16V7_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6258531.1"
                     /translation="PYPWPLDYVVRTREKKRVLDVMEEVGIPSPMDRYAAYPHQFSGG
                     MQQRALIATALVCGPALLLADEPTTALDVTIQAQIIDLMRRINQEHGTAIILVTHDLG
                     LAAEFCSRIAVMYAGRIVEQGTVDQMVENPQHPYTQGLLNCRPRISMRELRVEPIPGN
                     VPDLAELPEGCAFAPRCAHVQPACQAGPIPMVSIEADRLSRCLLHIGYHREDTWKWDH
                     MVNCP"
BASE COUNT          431 a          494 c          568 g          479 t
ORIGIN      
        1 gagatgccct gcatcatttc cagcagtggt tcagcttggg cgtgccaaga ttccagcatt
       61 tgctcctcaa ggtgtcccgt gttaagctcg accttggcaa gtttatctag tcggtgttgg
      121 atcgcagata aaatatgggg tttgaggtcg gtcacctggt gaccatgctg cttacccatt
      181 gcgaaatggt cccctgaaag atagagctgg cgcatcaaat ggatgtctcc gcagttttac
      241 ttagtgcttg attggtctgc tatgctgtgg tcttgataca gccagcatgc gacttgctgg
      301 ttatttacct gaaagaatgg cgggtattct tgttcacaaa taggtccaac tttggcagag
      361 cagcgtggat ggaagggaca cccggatggg gggtcaatcg ggctgggcac acctcctggg
      421 agaagaatgc gctccttgcg ggcagccttg ctgacccgcg ggattgccgc cagtaacgcc
      481 tgcgtgtagg gatgcagagg cgattcaaac aaatagtcag ttttacccag ttcgacgatg
      541 tgtcctaggt acatcactgc taccctgtcg ctgatgtagt agatcacgct caggtcatga
      601 gcaataaaca ggtaggtgag gtggtattct gcctgaagct cttccaacag gttgatcact
      661 tgagcttgaa tggaaacatc tagggatgaa accggctcat cggcaacgat aaattttggc
      721 cgcatggcta aggagcgggc gatgccaatc cgctggcgct gccctcctga gaactggtgt
      781 gggtagcggt cgatgtgtcg cttgctcaaa cctactcgtt gtaaaagttg ccgggtttct
      841 tcttcacgta gcagaggatc acgcacgcca cgttgttgta agggtgtgcc gatgatctcg
      901 cgcactgttt tgcgcgggtt cagtgacgag taggggttct ggaaaataat ttgggcaagg
      961 cgatgataat tcacgcgacg agtttggttg ctgccatcct gttgctcgag gatagtatca
     1021 ccttttacag gttgacctcg ataatagatc tggccgcgcg tgggttcata caggcgcgtg
     1081 agcacacgac cgagggtggt tttcccacag ccgctttcgc caaccagccc gagggtttca
     1141 ccgggccaga ttgccaggtc gacaccgtct acggcgcgga caaccttttc cttgacaccc
     1201 gctaagatcc gcgctagcaa actagagcgg atctggaagt gcatataaat tcccttccct
     1261 tcaaataagg gtgtgggtgt actctctgat tgtgcttcct tcatgggcaa ttcaccatat
     1321 gatcccactt ccaggtatct tcccgatggt aaccaatgtg gagcaaacat cggctgaggc
     1381 gatctgcctc tatgctcacc attggaatag ggcctgcctg gcaggcaggt tggacatgtg
     1441 cacaacgcgg ggcaaaggca caaccttcag gcagctcggc taaatcggga acattgccag
     1501 ggattggttc cacacgcagt tcgcgcatgg atatgcgcgg ccgacaattc agcagaccct
     1561 gggtgtaggg atgttgtgga ttttctacca tttggtccac ggttccctgt tccacaatgc
     1621 gaccagcata catcactgcg atgcggctgc aaaactcggc tgctaaaccc aggtcgtgcg
     1681 tcaccaggat gatagcggtg ccatgctcct ggttgatacg gcgcattagg tcgataattt
     1741 gagcctggat ggttacatcc agagctgttg tgggctcatc tgccagcagc agagctggtc
     1801 cgcataccag ggcagtggca atcagggcgc gctgctgcat cccaccagag aactggtgag
     1861 ggtaagccgc gtagcgatcc ataggggagg ggatacccac ttcctccatg acatctagga
     1921 cgcgcttttt ctcacgtgtg cgaacgacgt aatccagagg ccagggatat gg
//
LOCUS       DBNHOH010000848         7682 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_374706, whole genome shotgun sequence.
ACCESSION   DBNHOH010000848 DBNHOH010000000
VERSION     DBNHOH010000848.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7682)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7682)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7682
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_374706"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="AC16V7_15280"
     CDS             complement(<1..218)
                     /locus_tag="AC16V7_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258532.1"
                     /translation="MSAEIDIKQQVKDFYNQVGWQEVSQGVYQNAHYEDLRPVSRTYI
                     QRCHLRLLRHLRPSGCFLLDAGSGPIQYP"
     gene            complement(215..1822)
                     /locus_tag="AC16V7_15285"
     CDS             complement(215..1822)
                     /locus_tag="AC16V7_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258533.1"
                     /translation="MTKTIQLKPAIKHLLGEVPFAAETYWQIRQNGRPLSKSFSMHRT
                     QERLPAWRAAVEDCRRANPSGKHVLIFATLRYWIEHAALLGMTLAGLGHRVTLAYLPY
                     ANWNKPLSRFDIRRNNIYAQTVLGKAQPFIQPVSLLDIKHSDITLPQSLFDAIREVSV
                     RDVQYTLQVEDVDLNGELYHLRQQRNLQAARAALAWMKTSLPDVVLTPNGSILEMGAV
                     YQVARLLGIPVVTYEFGEQRERIWFAQNGEVMLQETDSLWQAHKDTILTEEQWQQVRA
                     LFTSRQNADLWQNFSRRWQGQPSQGGERVRSSLGLDSRPIVMLPANVIGDSLTLGRQV
                     YSQSMTEWLLRSVAYFAERPQVQLIVRIHPGERYTKGPSVAEVVSGAFHGSTLPEHIH
                     LVAADDPVNTYDLVEIADLGLVYTTTVGLEMAMSGVPVVVSGHTHYRDKGFTLDPGSW
                     EAYFQMLDDILEEPQKSRPSDEQVKQAWTYAYRFFFDYPRPFPWHLLHFWDELDTWPI
                     GRVLSPEGQEAFGDTFRDLLGEPVRLS"
     gene            complement(1855..3141)
                     /locus_tag="AC16V7_15290"
     CDS             complement(1855..3141)
                     /locus_tag="AC16V7_15290"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015784339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6258534.1"
                     /translation="MSDIAIRVENLGKQYVIGAQIDRYRTLRDTLVEVVKRPVRMLRG
                     GSGNTNDTIWALSGVSFEVRKGEVLGVIGRNGAGKSTLLKILSRVTEPSEGFAEIHGR
                     VGSLLEVGTGFHPELTGRENIALNGAILGMKRQEIERKFDEIVDFSGIEKFIDTPVKR
                     YSSGMYLRLAFAVAAHMEPEILVVDEVLAVGDAEFQRKCLGKMSDVASQGRTVLFVSH
                     NMSAILRLTQETILLEKGKLVLRAPSSQAVDAYLTSGFAQSGERYWQPDEVSSDAAPF
                     RPIALRVRNPQGQVVDAVRSTEATTLEIEYVLEAPISGLRVGIYLLTPQGEHIFTTFD
                     TDDANQYERFPERTAGHYVSRCSIPADFLNEGRYVVGVNASSFRVRRYFQDEHTLAFN
                     VDASGAPGTHWLENRLGLIRPRLEWKIEEIQGTRIP"
     gene            complement(3160..4008)
                     /locus_tag="AC16V7_15295"
     CDS             complement(3160..4008)
                     /locus_tag="AC16V7_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6258535.1"
                     /translation="MAEISQTAITEEVIVLRPSQGWSALNLRDLWLYRELIYFLTWRD
                     IKVRYKQTVLGAGWAILQPLINMIVLGVIFGNFAKMPTEGIPRPIFTFAALLPWGLFS
                     KALNDAGRSMLSNRNMITKVYFPRLIIPLASVLGGVVDFLIQFAILILMMVYYGYAPT
                     ANIWTLPLFLVLALITALGFGLWLSALNVLYRDVNYILPFLTQLWVLVTPVAYSSSVV
                     PEQWRMIYALNPMVGVVEGFRWALLGTETAPGPQMILSVVISLVLLITGMYYFRRMER
                     TFADMV"
     gene            complement(4070..5047)
                     /locus_tag="AC16V7_15300"
     CDS             complement(4070..5047)
                     /locus_tag="AC16V7_15300"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase family protein"
                     /protein_id="HIR6258536.1"
                     /translation="MSDTQSSNYWLNKRVIVTGGAGFLGSFLVEKLKVRGAAEVLIPR
                     VEQYNLVDIADIRRMYDDYILPTASNLQTIVIHLAALAGGIGANRSRPADFFYINLMM
                     GVQLMHEAWIRHIDKFVAIGTICAYPKFTPMPFREENLWDGYPEETNAPYGLAKKMLL
                     VQAQAYREQYGFNAIYLLPVNLYGPRDNFDLQTSHVIPALIRKFIEAQERGDRQVVLW
                     GDGSPTREFLYVEDACEGILLATERHDSAEPVNLGSGMEISIKDLAHLIARLTSYTGE
                     FVWDTSKPNGQPRRALDVTRAEKHFGFRAQMPFEEGLRRTIEWYRKNKG"
     gene            complement(5095..6144)
                     /locus_tag="AC16V7_15305"
     CDS             complement(5095..6144)
                     /locus_tag="AC16V7_15305"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6258537.1"
                     /translation="MKASSSRLDFGSEPAQRHILVTGGAGYIGSLLTGELLRVGYRVT
                     VLDDLLFGGESLLAYMSHPDFHFVKTNIWEPRAVRNAVRDDWSRPAAVVHLAAIAGFP
                     ACQAVGRQVAWRYNVEATQQVYEQAVELGVGRFIYASTYSNYGLSPDGNPVTEDTPLN
                     PQSLYAETKIAAERYLLGQASNPCAPLVFRLATLYGISPRTRFDMIVNQFILEAYTRR
                     ELLIYQRGYSRSFMHIRDAVRGILLGLKAPEADIRGQVYNLGTQGGNATKDQVVSLIL
                     KRLPETVVRYKDMTFGGDMRDISVSFEKVQSKLGFEAAYTLDDGVREVLHALRTGLIP
                     DPQNPRYRNAQFIVQ"
     gene            complement(6134..6631)
                     /locus_tag="AC16V7_15310"
     CDS             complement(6134..6631)
                     /locus_tag="AC16V7_15310"
                     /inference="COORDINATES: protein motif:HMM:NF024212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR6258538.1"
                     /translation="METPPETIHELNLLEHIEDDPDITQASLASQLGVAVGTVNWYLK
                     RLVAKGYVKVKRAQRRKLRYIITPEGIAFRARLTLHYIDTSMKLYRRTRQHVRDLLGD
                     VKKAGFNRVHIIGEGDIADVCRLTCLEQGISVAEMIDGKDADLPVIELHGMKVHLRLE
                     KVHES"
     gene            complement(6685..>7682)
                     /locus_tag="AC16V7_15315"
     CDS             complement(6685..>7682)
                     /locus_tag="AC16V7_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258539.1"
                     /translation="FRKRHLWQAFSPFLLWFAVAWLIFILLWPAMWVDPVGSLTSVFS
                     QATAYASEGHEIPSYFYGETQLGGISSWYFYPLVYLWRITPFILVGLVFVLLALFFPK
                     VFPLENTRLILVLLLFTGLFTIFMSLGAKKFDRYLLPIFAPLDLIAGLGWLALLKAAN
                     RYAKRLSTISRRLAGGLLLGIVLLGQLWSVIQSFPYYLNYFNPILGGSKAATQVMMVG
                     WGEGLDQAARYLNSLPQTGKERAISWYGDGCFSYFYDGVTVPIGLDFTFHDLRKTDIV
                     VLYLNQWQRGLPSPEFMAYFEQLTPDHVVRINNLEYVRVYNLRNAPEMPILTPGE"
BASE COUNT         1767 a         2138 c         2157 g         1620 t
ORIGIN      
        1 gggtactgga tcggcccgga gccggcatcc agcagaaaac acccggaagg gcgcaagtgg
       61 cgcagcaagc gcaggtggca gcgctggatg tacgtgcgcg aaactgggcg caggtcttcg
      121 taatgcgcgt tctggtaaac gccctggctc acttcttgcc agccgacctg gttgtagaaa
      181 tcttttactt gctgcttaat atctatctct gcgctcacga taatctcaca ggctctccaa
      241 ggagatcgcg gaaggtatct ccaaatgctt cctgcccttc gggagagagc acgcgcccaa
      301 taggccaggt gtccagctcg tcccagaagt ggagcaaatg ccaggggaac gggcgggggt
      361 aatcgaagaa aaagcgatag gcgtacgtcc acgcctgctt cacctgctca tcagagggac
      421 gggatttttg gggctcttcc aggatatcat ccagcatctg gaaataagcc tcccacgagc
      481 ctgggtccaa cgtgaagccc ttatcacggt agtgcgtgtg cccagatacg accacaggca
      541 caccgctcat cgccatctcc agcccgacgg tggtggtgta aaccagccct aggtcggcga
      601 tctccaccag atcataggtg ttgactggat catcagcggc aaccaggtgg atgtgctcgg
      661 gaagggttga tccgtggaaa gccccgctga ccacttcagc taccgaaggc ccttttgtgt
      721 agcgctcgcc aggatggatg cgcacgatta gttggacctg cggtcgctcg gcaaaatatg
      781 ctacgctgcg caacagccac tcggtcatgc tctggctata gacctgccgc cccagggtca
      841 agctatcacc gattacattg gcgggcagca tcacgatcgg gcgggaatcc aaccccaggc
      901 tggaacgcac acgctcgccc ccctgactgg gctgcccctg ccagcggcgc gagaaattct
      961 gccacaggtc ggcgttctgc cgggatgtaa acagggcgcg cacctgctgc cactgctctt
     1021 ccgtcaggat ggtgtcctta tgcgcctgcc acaaagaatc ggtctcctgc agcatgactt
     1081 cgccgttctg ggcaaaccag atacgctcgc gctgctcacc gaattcgtag gttaccaccg
     1141 ggatacccag caatcgtgcc acctggtaaa ccgcgcccat ctccagaatg ctaccgtttg
     1201 gggtgagcac cacgtctggc agactggttt tcatccaagc cagggcagcg cgggctgctt
     1261 gcaggttgcg ctgctggcgc aaatgatata gctccccgtt caagtctaca tcctcgactt
     1321 gcagggtata ctgcacatct cgtaccgaga cctcgcggat agcatcaaaa agactttggg
     1381 gcagcgtgat atcactgtgc ttaatatcca gcagcgagac aggttgaatg aaaggttgcg
     1441 ctttgccgag cacggtctgg gcatagatgt tattccggcg tatatcgaaa cgggaaagcg
     1501 gcttgttcca attggcatag ggcaggtacg ccagagtaac gcggtgcccc aatcctgcca
     1561 gagtcatccc caacagggcg gcgtgctcta tccagtatcg caaggtggcg aagatcagca
     1621 cgtgcttgcc cgacgggtta gctctgcgac aatcttccac ggccgcgcgc catgcaggca
     1681 agcgctcctg cgtgcggtgc atcgagaagc ttttgctcag tggtctgcca ttctgacgga
     1741 tctgccaata cgtctcggca gcaaaaggca cctcacccaa gaggtgtttg atggctggct
     1801 taagctggat agtttttgtc atcttggttg aaccgctcaa acctttaaag tctttcaggg
     1861 aatccgagtt ccctggatct cttcaatctt ccactccaac ctggggcgga ttaaccccag
     1921 gcgattttcc aaccagtgag tgcctggagc gccggaagca tctacattga aagccagggt
     1981 gtgctcatcc tggaagtacc gacgcacgcg gaatgagctg gcattgacgc ccaccacgta
     2041 gcgcccctcg ttgaggaaat ctgccgggat ggagcagcga ctgacgtaat gaccagccgt
     2101 tcgttcgggg aagcgctcgt attgattggc gtcatccgta tcaaaggtgg tgaaaatgtg
     2161 ctctccttgc ggggtgagca ggtagattcc cactcgcaga ccgctgatgg gggcttccag
     2221 aacatactcg atctccaagg ttgtcgcttc ggtggagcgc accgcatcca ccacctgccc
     2281 ctggggattg cgcacgcgca gggcaatcgg gcggaacggg gcagcatcgg atgagacctc
     2341 gtccggttgc cagtatcgct cgccagattg ggcaaagcca gatgtcagat aggcatcaac
     2401 agcttgagac gaaggggcgc gtagcaccag cttgcctttt tccaacagga ttgtctcctg
     2461 ggtaaggcgc aggatggctg acatgttgtg gctgacgaac agcaccgtgc gaccttggct
     2521 ggcgacatcg ctcatcttgc ccaagcattt gcgctggaac tcggcgtcac ccaccgccag
     2581 cacttcatcg accactaaaa tctccggctc catatgagca gcgacggcga aagccaggcg
     2641 caaatacatg ccgctggaat agcgcttgac gggggtatca atgaacttct cgatccctga
     2701 aaagtcgaca atctcgtcaa acttgcgctc gatctcttgt cgtttcatgc ccaggatagc
     2761 tccatttagg gcaatattct cgcgtccggt cagctctgga tggaaacccg tgcctacctc
     2821 tagaagggac cccacccgcc cgtgaatctc ggcgaagccc tcgctgggct ccgtgacgcg
     2881 cgaaaggatc ttgagcaggg tgctcttgcc ggcgccgttg cgcccgatta cgcccagcac
     2941 ctcgcctttg cgcacctcaa atgagacacc gctcagcgcc cagatggtat cgttggtgtt
     3001 gcctgacccg ccgcgcagca tgcgcacggg acgcttgacc acctccacta gggtgtcgcg
     3061 cagcgtccgg tagcggtcta tctgcgcacc aatcacgtat tgcttgccaa ggttttctac
     3121 gcggattgca atgtcactca tacaggttct ctgttccctt tacaccatat cggcgaacgt
     3181 ccgctccatg cggcgaaagt agtacattcc ggtgatcagc aagaccaacg agatgaccac
     3241 cgataatatc atctgcggac caggggctgt ctccgtgccc agcaaagccc agcggaagcc
     3301 ctcaaccaca cccaccatgg gattgagggc gtatatcatg cgccactgct ccgggacgac
     3361 gctggaggaa tacgccactg gcgtgacaag cacccaaagt tgcgtcagga agggcaggat
     3421 gtagttcaca tcgcgataga gtacattcag ggctgaaagc cacaggccaa aacctaacgc
     3481 cgtgattagc gccagcacca ggaacaaagg cagcgtccag atatttgcgg tgggagcgta
     3541 gccgtaatag accatcatga ggatcaagat ggcgaattgg atcaagaaat ccaccacgcc
     3601 gcctagcaca gacgcaagag ggatgatcaa cctggggaaa tagaccttgg tgatcatgtt
     3661 acggttggag agcatcgatc gtcccgcgtc attaagcgct ttggagaaga gcccccaggg
     3721 cagcagggca gcgaaggtga agattgggcg gggtatgcct tccgtgggca tcttggcaaa
     3781 gttaccgaaa atcactccta gaacgatcat gttgatcaac ggttgcaaga tagcccagcc
     3841 cgcccccaga acggtctgtt tgtagcgcac cttgatatct cgccaagtga ggaagtagat
     3901 cagctcgcgg tagagccaaa ggtcgcgcag gttaagcgcc gaccagccct gcgacgggcg
     3961 caggacaatg acctcttcgg ttattgctgt ttgtgatatc tctgccataa cacattccgt
     4021 gaagaagggg ataagggata ggggataggg gaaactgatc ccctatccct tatcccttgt
     4081 tttttcggta ccattcaatt gtcctgcgca acccttcctc gaagggcatt tgcgcccgga
     4141 agccgaagtg tttctcggcg cgggtcacat ccagggcgcg gcgtggttgc ccattgggct
     4201 tgctggtatc ccagacaaac tcacctgtgt agctggtcag gcgggcaatc aggtgagcca
     4261 ggtctttgat gctgatctcc atgcccgagc ccaagttgac aggctcggcg ctgtcatggc
     4321 gctcagttgc cagcaggatg ccttcgcagg cgtcctccac gtagaggaac tctcgagtgg
     4381 gtgagccgtc gccccaaagc accacctgcc ggtcgccgcg ttcctgggct tcgataaatt
     4441 tacggattaa agccgggatg acgtgcgagg tttgcaagtc gaagttgtcg cgcgggccgt
     4501 acaggttgac cggcagcagg tagatggcgt tgaagccata ttgctcgcgg taagcctgtg
     4561 cctgtaccaa cagcattttc ttggctaggc catacggtgc gttggtttcc tctgggtagc
     4621 cgtcccataa gttctcctcc cgaaaaggca tgggagtgaa ttttgggtag gcgcaaatgg
     4681 tgccaatggc gacaaactta tcgatatgac gaatccaggc ctcatgcatc aactgcaccc
     4741 ccatcatcag attgatgtag aaaaaatcgg cgggtctgga acggtttgcg ccgatgccgc
     4801 ctgctagggc tgccaggtgg atcacgatgg tttgaaggtt tgaggctgtt ggtagaatgt
     4861 aatcatcgta catgcggcgg atgtcagcga tgtccaccag gttatactgc tcaacccgcg
     4921 ggatcagcac ctccgccgcg ccgcgcacct tcaacttttc taccaaaaag gagcccaaga
     4981 agcctgctcc accggtaacg attacacgtt tatttaacca ataatttgaa gattgagtgt
     5041 cagacatcag aatttactcc ctcgtcacgg atgcggcgag cgcttgtgta attttcattg
     5101 aacgataaat tgggcattgc ggtagcgcgg attctgcggg tctgggatca gcccggtgcg
     5161 caaggcgtgc aagacctcgc gcacgccatc atccaaggtg tacgcggctt cgaagcccag
     5221 cttggactgc actttctcaa acgagacgga aatatcgcgc atatctccgc caaaagtcat
     5281 gtctttatat cgcaccacag tctcaggcag gcgtttcaag atcagcgaga caacctggtc
     5341 tttggtggcg ttgccgcctt gtgtgcccag attgtagacc tgaccgcgaa tatctgcctc
     5401 aggggctttc agacccagca agatgccgcg cacggcatct cgaatgtgca taaaggaacg
     5461 cgaatacccg cgctggtaaa tgagcaactc gcgccgggta taggcctcga ggataaactg
     5521 gttgacgatc atgtcgaagc gggtgcgagg agatattcca tacaacgtag ccagacggaa
     5581 taccagcggg gcacagggat tgctcgcctg accaagtagg tagcgctcag cagcgatctt
     5641 ggtctctgca taaagagact gagggttaag cggtgtatct tcggtcaccg gattcccatc
     5701 tggcgagagg ccgtagttgc tgtaggtgga agcgtatata aatcgaccta ctccaagctc
     5761 cacagcctgt tcgtaaactt gctgggtagc ctccacgttg tatcgccaag cgacctgtct
     5821 gcccaccgcc tggcaggcgg ggaaaccggc gattgctgcc aaatgcacta cagctgctgg
     5881 tctggaccag tcgtcgcgca cggcattgcg cactgcacgt ggctcccaga tgttggtctt
     5941 gacgaaatga aaatcgggat gcgacatgta ggccagcaag gattcgccgc cgaagagcag
     6001 gtcatccagt acggtgacgc gataaccaac ccgcaacaac tccccggtca gcagggagcc
     6061 gatatagccc gcaccgcctg tgacgaggat atgccgttga gcaggttcgc tgccgaaatc
     6121 tagtcgtgag ctgctagctt tcatgcactt tctccaggcg taaatgcact ttcataccat
     6181 gcagctcaat gacgggcagg tcagcatctt ttccatcgat catctctgcc acgctgatgc
     6241 cttgttcaag acaggtcaag cggcagacat cggcgatatc gccttcaccg atgatatgca
     6301 cgcggttgaa ccccgccttt ttgacatctc cgagcagatc acggacgtgt tggcgagtgc
     6361 gccggtacag cttcatcgat gtatcgatgt agtggagggt aaggcgcgcc cggaaggcga
     6421 tgccctcggg ggtgatgata taacgaagct tgcgccgctg ggcgcgtttt actttgacat
     6481 agcccttggc taccagacgc ttcagatacc agttcaccgt cccgactgcc acgcccaact
     6541 gcgatgccag tgaggcctga gtgatgtcag gatcatcctc aatgtgttct agcaggttta
     6601 attcgtgaat ggtttcaggt ggtgtttcca tgatatctaa aattcagccg ctgaattata
     6661 acatatatcg ggtatataca aggctcattc accaggggtt aagatgggca tctccggtgc
     6721 attccgcaga ttgtaaacac ggacgtactc caggttgttg atgcgcacca cgtggtctgg
     6781 agtgagctgc tcaaaataag ccatgaactc tgggctaggc aggccgcgtt gccactggtt
     6841 caggtagagc acgacgatat cggtcttgcg taaatcgtgg aatgtaaagt ccagcccaat
     6901 tgggacggta accccgtcgt agaaatacga aaagcagcca tccccgtacc aggaaatcgc
     6961 ccgctccttg cctgtttggg gcagcgaatt caggtagcgc gccgcttgat ctaagccttc
     7021 accccagccg accatcatga cctgggtagc tgcctttgag ccacctaaga tggggttaaa
     7081 gtagttcaaa tagtacggaa aggattggat tacactccac agttgcccca gtaggacgat
     7141 tcccaagagc aatccgcctg ctaaacgacg agagattgtg gacagtctct ttgcgtaacg
     7201 gttggctgcc tttaacaagg caagccagcc caagccggcg atcaagtcca acggagcaaa
     7261 gatcggcagc aggtaacggt caaacttctt tgctcccagg ctcataaaaa tggtgaagag
     7321 acctgtaaaa agcagcagca ccaggatcag gcgcgtgttt tccagcggaa atacttttgg
     7381 gaaaaacaaa gctaacagaa caaacaccag cccaaccaag ataaaggggg tgatgcgcca
     7441 caagtacacc agcgggtaga agtaccatga ggatatgcca cccagctgag tttccccgta
     7501 gaaatagctg ggtatttcat gcccttcgct ggcatacgct gtggcttggg agaagacgct
     7561 ggtcaggctg cccacgggat ccacccacat ggcaggccag agcagaataa atatcagcca
     7621 agccactgca aaccagagca ggaatggtga gaaagcttgc cacaggtgcc gctttcggaa
     7681 aa
//
LOCUS       DBNHOH010000849         5396 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_53993, whole genome shotgun sequence.
ACCESSION   DBNHOH010000849 DBNHOH010000000
VERSION     DBNHOH010000849.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5396)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5396)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5396
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_53993"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="AC16V7_15320"
     CDS             complement(<1..186)
                     /locus_tag="AC16V7_15320"
                     /inference="COORDINATES: protein motif:HMM:NF012759.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR6258540.1"
                     /translation="MNSYTTELTMNVCNLLASALQVPADSVSAELSFGDLPEWDSMGH
                     MEVMMQLEEHFGVEVSAD"
     gene            complement(232..1113)
                     /locus_tag="AC16V7_15325"
     CDS             complement(232..1113)
                     /locus_tag="AC16V7_15325"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6258541.1"
                     /translation="MEDKIEILSVELLDQHRPTVRALKRLAGSLGLEFGWHYLLDLTW
                     IISLLGAVKGKRILDAGAGTGVLQWYLAEQGAEVISVDRLSRANLPLRFRRRYNVRGL
                     RSQDLLPSATILRQSFTERTKLQLKVKSQVRDLAGLTDLRRVPGRVIIYNQELQNLSD
                     LADASIDAVVAVSALEHNPPEGLPGVVIELQRVLKPDGVLLATLGAARQADWLHVPSQ
                     GWCYTDASLRRHFDLPADTLTNYDRHDDLFSALRDCAELRDGLASFYYQSGDNGMPWG
                     RWDPQYQPVGVCKVKRG"
     gene            complement(1117..2769)
                     /locus_tag="AC16V7_15330"
     CDS             complement(1117..2769)
                     /locus_tag="AC16V7_15330"
                     /inference="COORDINATES: protein motif:HMM:NF017011.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide export protein,
                     LipB/KpsS family"
                     /protein_id="HIR6258542.1"
                     /translation="MPAQNSLLSRLSLGYAGWQNRARIAQLARQVAAHSPTSTQPSAD
                     PRPVVLFNASTRLTGLSLNAAFQLLTGWGLRLAGAPVVHFVCQSGLQPCVLGTNRQDY
                     TTPPPCDSCLAQSRRLYAGANVHGFGLKLDPQLSTALQNLSVDELGTFEYQLPALSDQ
                     HSPIPLGRLVVPSIRWSLRRHNLPDDEPTRYLLRHYILSAYNVAVEFAALLDQLQPQS
                     ALIFNGAMYPEATARWVARQLEVRSVAHEVGFQRFSTFFTEGEPTAYPIHIPDDFELS
                     EAQNDQLDAYLEQRFQGKFSMAGIQFWPEMRGLDETLLQKTASFRQVVPVFTNVVYDT
                     SQVHANVIFPNMFAWLELVTEVIRAHPETLFVIRAHPDEMRPGTKKLSNESVHDWIHS
                     RGIHQWPNVVFIGPLEYLSSYELIHQAKFVIVYNSSIGLEATLLGVPVLCGGKARYTQ
                     YPTVFFPQSPEAFRQQANEFLKAESISVPIEYERNARRFLYYQLYRASIPLDDYLQTG
                     PRPGFVLLRDFSWRQLQPENSVPMRVLVEGILEEKPFLMPEN"
     gene            complement(2818..4707)
                     /locus_tag="AC16V7_15335"
     CDS             complement(2818..4707)
                     /locus_tag="AC16V7_15335"
                     /inference="COORDINATES: protein motif:HMM:NF015087.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HIR6258543.1"
                     /translation="MPHIKIGDQLIGDDQPTYIIAEIGVNHNGLLDLALKLIDIAKDA
                     GADAVKFQKRKLDTLYSKKYLDNANAGEKTLRYLLPILQQVELSEEAFYKILEHCQAQ
                     EITFMCSAFDAESADFLDGLGVPAYKVASADLTNLPLLDHLASKGKPLILSTGMSRME
                     EVELTVDFLQQRKAEFALLHCNSTYPAAFEDINLRFMDQLRRFGVPVGYSGHERGIAV
                     STVAAAMGASIIERHLTLDRTMDGPDHAASLEPQGFHKMVRDIRQVFQALGTGEEKFF
                     SRGEILNREVLGKSLVAARRIEPGEVIHAAMVAVKGPALGISPQRYPELVGRTAERVI
                     EEDEPFQESDLGHKILLDVEHTLPMEYGFTVRFRDFGELLAYKPRMLEYHFTDQDLDE
                     HYPGGDHNLKLVSHAPEFWDRTLVDLCSLDERQRCDSVKLMQKTIDLTREMAPHFVGT
                     PKVVIHPGAMSLDHPIKDREKLYVNLRRSVNELDFEGVELLLENLPPHPWYFGGQWLT
                     NAFMDAREIRDFITSMGLNMCFDTSHAKLYCNWSHADFYEQVQILLPYIRHLHLSDGA
                     GLDGEGLQIGEGTIDWVHFFKVTGDYHGTMIPEIWRGHQRKGEGFLLAIQRLSEAYFA
                     ARDNA"
     gene            complement(4771..>5396)
                     /locus_tag="AC16V7_15340"
     CDS             complement(4771..>5396)
                     /locus_tag="AC16V7_15340"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HIR6258544.1"
                     /translation="TRVIVSTDDDEIAAVARRYGAETPFLRPDEYSQDNTPDLPVFQH
                     ALNWLEEHDGYRPEIVVHLRPTSPFRRVSHIDEAVYHLMARPEADAVRTVCVPFQNPF
                     KMWRIEPDGFMRPLGVELGISHEPYNQPRQMLPEVYWQTGYVDVAWSDTILVKDSMTG
                     ERILPLIIDPSQWIDIDSADDWRRAERLLESGEITFEDLGYKIENRE"
BASE COUNT         1151 a         1479 c         1618 g         1148 t
ORIGIN      
        1 atcggcgctg acctccacgc caaagtgctc ttcgagctgc atcatcacct ccatgtgacc
       61 catcgaatcc cactcgggca ggtcaccaaa actcagctct gcgctaaccg agtctgcggg
      121 cacctgtaag gctgaagcaa gcaaattgca gacattcatg gtcaattcgg ttgtataact
      181 attcatgtaa catccttgag agtagatcag caatcagcaa tcagaagttc cttatcccct
      241 cttcactttg caaactccaa ccggctggta ttgcgggtcc caacgtcccc agggcatgcc
      301 gttatccccc gactggtaat agaaagacgc caggccgtcg cgcagctcgg cgcaatctcg
      361 cagtgcacta aaaaggtcat cgtggcgatc atagtttgtg agggtgtccg ctggcaggtc
      421 aaagtggcgg cgcaggctgg catcggtgta gcaccaccct tgggaaggga catggagcca
      481 gtcagcttgg cgggcggctc ccagggtcgc cagcagtacg ccatcaggct tgagcacccg
      541 ctgtaattca atgaccacac cgggcagccc ttctggaggg ttatgctcca atgccgatac
      601 ggccactact gcgtcaatgc ttgcgtccgc cagatcgctc aaattctgca actcctggtt
      661 gtagatgatc acccgccctg gtacccggcg gagatcggtc agtccggcta ggtcgcgcac
      721 ctgtgatttc actttgagct gtagcttagt acgctcagta aaagactgtc gcaaaatcgt
      781 tgcgctgggg agcaggtctt ggctgcgcag gccacgcaca ttgtaacgtc tgcgaaaacg
      841 aagcggcagg tttgcgcggc tgagacggtc cacactgatc acttcggcac cctgttcagc
      901 cagataccac tgcaagacgc cagtgccggc gcctgcatcc aagatgcgct tgcctttgac
      961 tgcccccagc agggagatga tccaggtcag gtctagcagg taatgccagc caaattccag
     1021 acccaaagac cctgccaggc gcttgagcgc gcgcactgtt gggcggtgtt ggtctaataa
     1081 ctctacggac aaaatttcga ttttgtcttc catgggctaa ttctcgggca tcaaaaatgg
     1141 tttctcctcc aggattcctt ctaccaggac tcgcatcggg actgaattct caggttggag
     1201 ctggcgccag gaaaaatctc gcagtagtac gaatcctggg cgcgggccgg tctgaagata
     1261 gtcatccaga gggatggatg cccggtagag ctggtaatac aggaacctgc gggcgttgcg
     1321 ctcatattct attggcacag agatcgattc agccttcaaa aattcgttag cctgttggcg
     1381 aaaagcttct gggctttgcg gaaagaaaac ggtggggtat tgcgtgtagc gcgctttccc
     1441 gccgcacaat acaggaacgc ccaatagcgt cgcctccagc ccaatggacg agttgtagac
     1501 gatcacaaac ttggcctggt gaatgagctc atacgagctg aggtattcca aaggtccgat
     1561 gaaaaccacg ttgggccatt ggtgtattcc acggctgtgg atccaatcgt gcacgctctc
     1621 attactcagt ttcttggtgc cggggcgcat ctcgtcaggg tgggcgcgga ttacgaagag
     1681 cgtctctggg tgagcgcgga tcacctcagt gaccaattcc agccaggcga acatgtttgg
     1741 gaagatcacg ttggcgtgca cctggctggt gtcgtagacc acgttggtaa acaccggcac
     1801 tacctggcgg aagctggcgg tcttctgcag cagtgtctcg tccaggccgc gcatctctgg
     1861 ccaaaactga atgcctgcca tgctgaactt accttggaag cgctgctcca ggtaagcgtc
     1921 cagctggtcg ttttgcgcct cgctcagctc aaaatcgtct gggatgtgaa tggggtaagc
     1981 agtcggctcg ccctcggtga aaaaggtgga gaagcgctgg aaacccacct catgggcgac
     2041 ggagcgcact tccagctgcc gtgccaccca gcgcgcagtg gcttcgggat acatggcgcc
     2101 attgaaaatc aaggcactct ggggttgaag ttggtcgagc aaggccgcga actctacagc
     2161 cacattgtat gctgagagga tatagtggcg caggagatag cgggtgggct cgtcgtcggg
     2221 cagattgtgg cggcgtaatg accaacggat agaagggacg accaggcgcc ccaaagggat
     2281 cggggagtgc tgatcgctga gtgccggcaa ttgatattcg aaagtgccca gctcatctac
     2341 gcttagattt tgcagagcgg tcgagagctg cgggtcaagc ttcagcccaa atccatgaac
     2401 attggcccct gcatagagac ggcgggattg cgctaggcat gagtcgcatg ggggtggtgt
     2461 agtatagtcc tggcggtttg tgcccaaaac acagggttgc agccccgact ggcagacgaa
     2521 gtgcaccacc ggtgcgcccg ccagcctcaa accccagcca gtgagcagct ggaatgcggc
     2581 gttaaggctc aagccagtca gccgcgtgga ggcgttgaac aggaccacgg ggcggggatc
     2641 ggcagatggc tgggtggagg tgggggaatg agcggcgacc tggcgcgcta gttgggctat
     2701 gcgggcgcgg ttctgccagc cggcatagcc caggctgaga cgagaaagaa gactgttttg
     2761 agcaggcatg cttggggtcg ataaattaac agtgtagctg gtgtctttgg ttcagcctca
     2821 tgcattgtcc ctggctgcga agtaggcctc agatagccgt tgaatagcta gcaagaaacc
     2881 ctcccccttg cgctggtggc cgcgccagat ctcggggatc attgtgccgt ggtaatcacc
     2941 ggtcactttg aagaaatgca cccaatctat ggtgccctcg ccaatttgta atccctcgcc
     3001 atctagaccc gctccgtctg agagatgcaa atggcggata tacggtaaca ggatttgcac
     3061 ctgctcgtaa aaatcggcgt gagaccagtt gcagtacagc ttggcgtggg aggtgtcgaa
     3121 acacatgttc aggcccatag aggtgatgaa gtcacgaatt tctcgggcgt ccatgaaagc
     3181 gttggtaagc cactgcccgc cgaagtacca gggatggggc ggcaggttct ccaaaagcag
     3241 ttcgacccct tcgaaatcta gctcattcac agagcggcgc aggtttacgt acagcttctc
     3301 gcgatcctta atcgggtgat ccaggctcat cgctccaggg tgtatcacta ctttaggtgt
     3361 gccgacaaaa tggggcgcca tctcgcgcgt caggtcgatg gtcttctgca tcagcttcac
     3421 ggagtcacag cgctggcgct cgtccagcga gcagaggtcg accagcgtgc gatcccagaa
     3481 ctctggggcg tgtgatacca gcttcagatt gtgatcccct ccggggtagt gctcgtctag
     3541 gtcttggtcg gtaaagtgat attccagcat gcgcggcttg taagccaaca gctctccgaa
     3601 atcacgaaag cgcacggtga agccatactc catcggcagg gtgtgttcta cgtccaggag
     3661 gatcttgtga cccaagtcgc tctcctggaa tggctcgtcc tcttcgataa ctcgttcggc
     3721 agtgcgtccc accagttcag ggtagcgttg cggcgagatg cccagcgccg gtcccttcac
     3781 cgccaccatg gctgcatgga tcacctcgcc gggctcgatg cgccgggcgg cgaccagact
     3841 cttgcccagc acctcgcggt tgaggatctc accgcgcgag aagaatttct cctcgccggt
     3901 gcccaatgcc tggaagacct ggcggatatc gcgcaccatc ttatggaaac cttgtggctc
     3961 caggctggca gcgtgatcag gaccatccat ggtgcggtcc agggtaagat ggcgttcgat
     4021 gatgctggcg cccatagccg cagccacagt ggagacagcg atgccgcgct cgtgccccga
     4081 gtagccaacc ggcacgccaa agcggcgcaa ctggtccata aagcgcaggt tgatatcctc
     4141 gaaggcagcc ggataggtac tgttgcagtg cagcagggcg aactccgctt tacgctgttg
     4201 taggaagtcc acggtgagct ccacctcctc catgcgcgac atacctgtgg agaggatgag
     4261 cggcttgccc ttgctcgcca ggtggtccag caggggcagg ttggtcaggt cggcagaggc
     4321 tactttatag gcgggcacac ccaggccgtc caggaagtcg gcgctctcgg cgtcgaacgc
     4381 cgagcacata aaggtgatct cctgcgcctg gcaatgctcc aggattttgt agaatgcttc
     4441 ttccgagagc tcaacctgtt gcaagatggg cagcaggtag cgcagtgttt tctcgcctgc
     4501 attggcgtta tccagatatt tctttgaata gagtgtatcc agcttgcgct tctggaactt
     4561 gaccgcgtcg gcgccggcgt ccttggcgat gtcaatcagc ttcaaagcca aatcgagcag
     4621 gccattgtga ttgacgccaa tttccgcgat gatgtaagta ggttgatcat cgccgatcaa
     4681 ctggtcgccg atcttaatat ggggcatgaa aacctccagt tcgtcgagat tagggagtgg
     4741 agagtatgac acaggttata cgagacgtgt ctactctcga ttctcgattt tgtatcctaa
     4801 atcttcaaag gtgatttcgc cgctctccag cagccgctcg gcgcggcgcc agtcgtcggc
     4861 ggagtcgata tcgatccact ggctaggatc gatgatcaag ggcaagatgc gttctccggt
     4921 cattgagtcc ttgaccagga tggtgtctga ccaggcgaca tccacatatc cggtctgcca
     4981 gtagacttct gggagcatct ggcggggctg gttgtatggc tcgtgagaga tgcccagctc
     5041 tacacccaag gggcgcatga aaccatctgg ctcgatgcgc cacatcttga acggattttg
     5101 gaatgggacg cacacggtac gcacggcgtc ggcttcgggg cgcgccatca agtggtaaac
     5161 cgcctcgtcg atatgggaga cccgccggaa cggagaggtg gggcgcaggt gcaccacgat
     5221 ttcagggcgg tagccatcat gctcctccag ccagttgagt gcatgctgga agacaggcag
     5281 gtccggggtg ttgtcttgcg agtactcgtc cgggcgtagg aagggcgtct cagcaccata
     5341 gcggcgagcc acggcggcaa tctcgtcgtc atcggtagag acgatgaccc gcgtaa
//
LOCUS       DBNHOH010000850         1251 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1465251, whole genome shotgun sequence.
ACCESSION   DBNHOH010000850 DBNHOH010000000
VERSION     DBNHOH010000850.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1465251"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="AC16V7_15345"
     CDS             <1..1176
                     /locus_tag="AC16V7_15345"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR6258545.1"
                     /translation="SLAQSFNTLLAQIKLKLEGMENTVSKRDQLSEHHSNQLRALSEV
                     SQTIVTTRIIDELLPKIVQLISQHFGYSHIGIYLIDATGEYAILQAMISKEGESISAY
                     AQKIQVAEGGIIGYVINNKEHRIVEDMLEDDLYTNNPDFPDTRSEIAIPLLGGDQVIG
                     VLDIQSREPNAFAQDDITTLLLIADQVAIAIDNARLFRENQGALESIRRAYSQLSQDA
                     WAKLLHEQPELGYLANRLDISYTPANGWHPEEVQAAQTGSTIQVSTDTLAIPIKERDH
                     VLGILRLRKPDSHMWSKDELALAETLAQQLYLALENARLYQETQRRAERERLASEITA
                     KLRASNDPQVIMQTAIQELRRVLNIRSEEVPPNAALRSTDHMPRQQDASSQESQEIG"
BASE COUNT          378 a          324 c          278 g          271 t
ORIGIN      
        1 tcgctggctc aatcgtttaa caccctgctg gctcaaatta aattaaaact cgagggtatg
       61 gaaaatacgg tttcaaaacg cgatcagtta tcggaacatc attccaatca actgcgcgct
      121 ttgtcagagg taagccaaac catcgtcact acccgcatta ttgacgagct cctgccaaag
      181 attgtacaac taatcagcca gcacttcggc tactcacaca tcggcatata cctgattgac
      241 gcaaccggtg aatacgcaat acttcaagca atgatcagca aagaagggga gagcataagc
      301 gcttacgccc aaaaaatcca agttgccgaa ggaggaatca tcgggtatgt aattaataat
      361 aaggaacatc gtatcgtaga agacatgctt gaagatgacc tatatactaa taaccccgat
      421 tttccagaca cgcgttctga aatagccata cctttacttg gtggtgacca agtaatcggg
      481 gtgctggata ttcaaagcag agagccaaat gcatttgccc aagacgatat caccacattg
      541 ctccttattg ccgaccaggt tgctattgct atcgacaatg cccgcctgtt ccgcgagaat
      601 caaggggctc tcgaatccat tcggcgcgct tacagtcaac taagccaaga tgcatgggca
      661 aagcttcttc atgagcaacc cgaattgggc tatttggcta accgcttgga tattagctat
      721 acaccggcga atggttggca ccctgaagag gtccaagccg ctcaaaccgg tagcacaata
      781 caagtcagca ccgatacact cgccatcccc ataaaggaac gagaccatgt tttgggaata
      841 ttgcgcttgc gcaagcccga ctcccatatg tggtctaaag atgagctcgc attggcagag
      901 acactcgccc aacagcttta cctggctttg gaaaacgccc gtctgtatca agaaactcaa
      961 aggcgcgccg aacgcgaacg cctcgctagc gaaataaccg caaaattgcg cgccagcaat
     1021 gatccccaag tgataatgca gaccgcaatt caagaattgc gacgggtatt gaatatccgt
     1081 agtgaagaag tccctcccaa tgcagctcta cgatcaacag atcacatgcc acgtcaacaa
     1141 gacgcttcaa gccaggaaag ccaagagatt ggatgaatat gacctacata atagctgttg
     1201 ctaatcaaaa aggcggtgtt gccaagacca cctcagcggt gtccctgggt g
//
LOCUS       DBNHOH010000851         1351 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1143784, whole genome shotgun sequence.
ACCESSION   DBNHOH010000851 DBNHOH010000000
VERSION     DBNHOH010000851.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1143784"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="AC16V7_15350"
     CDS             <1..601
                     /locus_tag="AC16V7_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR6258546.1"
                     /translation="ISLYQSDPNPYWYTSATSLLDEMIQHFSDPSGGFFDTRIDHEPL
                     ITRPKDLQDNATPCGNSLAAMALLQHSNYSGRSDLVDIAETSLRSIIDQSSKYPTAFA
                     CWLCAADYVANPVSEVAIIGSAKDERTQALVNVLWETYRPRTLSAQANLPISTEMPEL
                     LDNRELVDGLPAAYVCQNFVCNLPVTTPDELRIQLKRAA"
     gene            598..1161
                     /locus_tag="AC16V7_15355"
     CDS             598..1161
                     /locus_tag="AC16V7_15355"
                     /inference="COORDINATES: protein motif:HMM:NF013122.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR6258547.1"
                     /translation="MISSRNQAIQLAKEKIKSNPVYLDTETTGYARDSEIVEVCVLDD
                     QGEVLFESLVKPTQPIPRDAVRIHGITNEMVSSAQSWMSVWPNLERVLSGHVVGIYNA
                     EFDLRLIQQTHNKYRMKWASRADFTPFCIMKLYAQYYGEWNHSRGSYRWHSLEDAGRQ
                     CHIPLPNSHRARDDCLLARALLHFMAS"
     gene            complement(1158..>1351)
                     /locus_tag="AC16V7_15360"
     CDS             complement(1158..>1351)
                     /locus_tag="AC16V7_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR6258548.1"
                     /translation="RRLYPGRVQYTWWTYVGRARERNTGWRLDYFLVSENLMPRVQDV
                     VIYDDIPGSDHCPVELFID"
BASE COUNT          360 a          347 c          296 g          348 t
ORIGIN      
        1 tatctccctc tatcaatccg atcccaaccc ctactggtat acctctgcga cgtccttgct
       61 agacgagatg atccagcact tctcagatcc ttcaggcggt ttcttcgata cgcgcatcga
      121 tcatgaacca ctgattaccc gccccaaaga tttacaagac aatgccaccc cctgtggaaa
      181 ctctctagca gcgatggcat tgttacagca ctcaaattat agcggtcgga gtgatctggt
      241 tgatattgcg gagacttccc ttcgctcaat tattgatcaa tccagcaagt atccgacagc
      301 atttgcttgc tggctatgcg cagcagacta tgttgccaat ccggtttctg aagtggcgat
      361 aatcggatct gctaaagatg agcgcactca agcattagtg aatgtgcttt gggaaaccta
      421 tcggcctcgc acgctgagcg cccaagccaa tttaccaatt tctactgaaa tgcctgaatt
      481 gcttgacaat cgagagctcg tcgatgggct tcctgcagcc tatgtctgcc aaaactttgt
      541 ctgcaatttg ccggttacca caccagatga actacgcatt caactgaagc gtgctgcatg
      601 atatcgtccc gtaatcaagc tatacaactc gcaaaggaaa aaattaaatc caatccggtg
      661 tatttggaca ccgaaacgac aggatacgcc agggacagtg agattgttga agtttgtgta
      721 ttagacgatc aaggggaagt tctgtttgag tcattggtca agccaaccca gcctattcca
      781 agagatgcag tcagaattca cggtatcaca aatgaaatgg tcagttctgc ccaatcatgg
      841 atgtcagtat ggcctaatct agaaagggtg ctctctggtc atgtggtagg catttacaac
      901 gccgaatttg acctgcgatt aatccagcag acacataaca aataccggat gaaatgggcc
      961 tcccgtgcag attttacgcc attttgtatt atgaaattgt acgctcagta ttacggagag
     1021 tggaatcatt ctagggggtc ttatcgctgg cattcactag aagacgctgg tcgtcagtgc
     1081 catatccccc tgcccaattc ccaccgtgct cgagatgatt gcctgcttgc gcgcgcactg
     1141 ctgcacttca tggcttccta atcaataaat aattctaccg gacaatgatc tgagccaggg
     1201 atatcatcat agatgacaac atcctgtact cgaggcatca ggttttccga aaccagaaag
     1261 taatcaagtc tccaacctgt atttcgctca cgcgctctgc caacataagt ccaccaggta
     1321 tactgaacac gccctggata gagcctgcgg t
//
LOCUS       DBNHOH010000852         1749 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_566160, whole genome shotgun sequence.
ACCESSION   DBNHOH010000852 DBNHOH010000000
VERSION     DBNHOH010000852.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_566160"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="AC16V7_15365"
     CDS             <1..241
                     /locus_tag="AC16V7_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258549.1"
                     /translation="ISTLVIAASFAPLRRRVQNLIDRRFYRSRYNAARTIENFGVGLR
                     EEVDLSTLSERLVAVVDETMQPEFVNLWMRKGEGK"
     gene            238..390
                     /locus_tag="AC16V7_15370"
     CDS             238..390
                     /locus_tag="AC16V7_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258550.1"
                     /translation="MSALWATILKERNRASIRVAEKTGMAFEKEGRDELGPFHLYAIN
                     QLERTN"
     gene            387..1643
                     /locus_tag="AC16V7_15375"
     CDS             387..1643
                     /locus_tag="AC16V7_15375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258551.1"
                     /translation="MTKEKRNAIFAWIFFGLIAASNIVIIIYYLAAFPGEKPTLVELT
                     FPFVPVLFAFVGALIISRQPRNVIGLVMMLPGLSLAFVIDASLSPYLSGQLPPPATPS
                     PLFLIILWFSNWNWLLLVLPVMFIMLLFPTGQSLSPRWKWLVYFGLGIVVVFVFLITF
                     AKVLAPNKDPIPWSVPNPIGFLKPEWINTIVAPFMILFPVWILLCAVSLFIRFRRARA
                     VEREQIKWLFYAGALFVVFYMPSFIGNTYSQAESFWNLLLPIGMSTFPIAIAIAILRY
                     RLWDIDILIRRTLVYTILTAMLGLVYFGLVITLEGAMRALVGEGGQIATVISTLSIAA
                     LFTPLRRRVQETIDRRFYRQKYNAEQALAQFAAAARDETDLEALTARVVSIVQDTMQP
                     EQVSLWLQPTASQRKSSLDTGQIGPK"
BASE COUNT          331 a          496 c          459 g          463 t
ORIGIN      
        1 catctccacc ctggtgattg cggcttcttt tgccccactc cggcgcagag tacaaaactt
       61 gattgaccgc cgcttctatc gcagtcgcta taacgccgct cgcacgatcg agaacttcgg
      121 cgttggattg cgcgaagagg tggatctgag cactttgagt gagcgattgg ttgcggttgt
      181 cgatgagact atgcagccag aatttgttaa cctgtggatg cgcaagggtg agggaaaatg
      241 agcgcattgt gggcaacgat cctcaaggag cgtaaccggg cttcgatccg ggtggccgaa
      301 aaaaccggga tggccttcga gaaggaaggt cgcgacgaac tggggccttt tcatttgtat
      361 gcaatcaacc agttggagcg cacaaattga caaaagaaaa acgcaatgcc attttcgcct
      421 ggatattttt cggattgatt gccgcttcca acattgtgat catcatttac tatctggcgg
      481 cattccctgg ggagaagcct accctcgttg agttgacatt tcccttcgtt cctgtgttgt
      541 tcgcatttgt cggcgcgctg atcatttcac gccaaccccg caacgtgatc ggcctggtga
      601 tgatgctccc tggcctctcg ctggcttttg ttattgatgc ttctctgtcc ccatatctca
      661 gtggccaatt gccgcctcct gccaccccct caccgctttt tctcatcatt ttatggttta
      721 gcaactggaa ctggcttttg ctggtcctgc cggttatgtt catcatgctc ctcttcccga
      781 caggtcagtc gctcagcccg cgctggaaat ggttggttta ttttggcctg ggtatcgtgg
      841 tggtatttgt atttttgatc acctttgcca aggtgttggc acccaacaag gacccgatcc
      901 cctggtctgt gccaaacccg attggctttc tgaaacccga gtggatcaat actatcgttg
      961 caccgtttat gatcctcttc ccggtttgga ttctcctatg cgctgtctcc ctgtttatac
     1021 gctttcggcg ggcacgggcc gtggaacggg aacagattaa gtggctgttt tacgccgggg
     1081 cgctcttcgt tgtattctac atgccttctt ttattggtaa cacctacagc caggccgaaa
     1141 gcttctggaa tttgctgctc cccatcggca tgtcgacgtt ccctatcgcc atcgccatcg
     1201 ccatcctgcg ctaccggctg tgggacatcg acatcctgat tcgccgcacg ctggtctaca
     1261 ccatactgac cgccatgctt ggcctggtct attttggcct ggtgatcacc ctggaggggg
     1321 ctatgcgggc tcttgtgggc gagggcggtc agattgccac ggtgatctcc accctgagta
     1381 ttgctgcttt attcaccccg ctgcgccgcc gcgtgcagga gaccatcgac cgccgcttct
     1441 accgccagaa gtacaacgct gagcaggccc tggcacagtt cgctgccgcc gcccgggacg
     1501 agactgacct ggaagccctc accgcccggg tggtgtctat cgtgcaggac accatgcagc
     1561 ccgaacaggt cagcctgtgg ctgcagccaa ctgcaagcca gagaaaatcc tcattagata
     1621 cggggcaaat tggaccaaaa tgacagctcg aacctatcct gaaaagcgcc ttaaccgctc
     1681 agcctgggtg gtcatgtttt tttcgctggt gatgatggtc tacgccatcc tggcgatggc
     1741 ttattacct
//
LOCUS       DBNHOH010000853         2472 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_566346, whole genome shotgun sequence.
ACCESSION   DBNHOH010000853 DBNHOH010000000
VERSION     DBNHOH010000853.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_566346"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="AC16V7_15380"
     CDS             <1..759
                     /locus_tag="AC16V7_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258552.1"
                     /translation="LWSSVGYGSYRQTGPMLIEIPLPRMKEGQILSGTAPLYSILEDI
                     FGIVVKPSTWLLPTVADLVFGTILLLLIWLIYRFEVIKPGFFISQGFLILSIMLIIGL
                     VLSPTVVLASSKMERVCYGDVIASYESVGKHLASLIPPGSSVYWEGGLSVVPLLYLPG
                     IRIYPAQINDGYSFRIGGNAEELQRAGLWISELQTRWLSEADFILVEQDRYSKAINYW
                     TEILKPDRFNELEISPSFLPCRETRLRVFQRIEQ"
     gene            756..1742
                     /locus_tag="AC16V7_15385"
     CDS             756..1742
                     /locus_tag="AC16V7_15385"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR6258553.1"
                     /translation="MKINLSAFAKKQSKILRIVGTVTALALLIYVLHEQGWEEIGNAV
                     KQIDVWRLGLALLMMIISRLAVSGRWYILLRSGDAPITPKECFQITFAGLFANNFMPT
                     TVGGDVIRLAGALRLQCDTAISAASLIADRLVGMAGMLMILPVGLPRLLQIHDLRDLI
                     PTNAEVIQFSLAATPLGDLPRKIFGRLQSILHRLLSTLTVWLRQPRALLLSLLFTWIH
                     QICLFSALTLFFSGMGESISFWMVGGLYSFVYLITLIPISINGYGLQEVSMTLVFSRL
                     GGASLASSLTAALLLRTAVMVASLPGAAFVPSLLAYSRERPETKNAPQTDIV"
     gene            1763..>2472
                     /locus_tag="AC16V7_15390"
     CDS             1763..>2472
                     /locus_tag="AC16V7_15390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR6258554.1"
                     /translation="MKLSVVIPVYNEAENLPVLQKALSNCFGGQDRDWEVIYVDDGST
                     DVSLTILEGFASEEPEHTSVVSLRRNFGQTAAIAAGIDHANGDIIVLMDADMQNDPAD
                     IPKMLEKIEEGYDVVSGWRKNRQDTFITRTLPSRIANWLISTITGVHLHDYGCTLKAY
                     RREALTGFRLYGEMHRFIPAYAHAVGATIIELPVTHHPRKFGKTKYGLNRTIKVILDL
                     FTVKFLISYAHKPIYLFGG"
BASE COUNT          588 a          606 c          604 g          674 t
ORIGIN      
        1 ttgtggtcaa gtgtgggcta tggatcttac cgacagacgg gcccgatgct gatagagata
       61 ccattgccac ggatgaaaga agggcaaata ctgagcggta cagctccgct ctactcaatt
      121 ttagaggata tctttggcat agtagtgaag ccttcgacat ggcttttgcc gaccgtcgcc
      181 gaccttgtct ttggtacaat cctattgctc ttgatttggc tgatctatcg ctttgaagtt
      241 attaagcctg ggttttttat aagtcaaggc ttcctgatat tgagtattat gctcatcatt
      301 ggtctagtat tatcacccac cgttgtgcta gcgagttcca agatggaacg tgtatgctat
      361 ggagacgtga ttgcatccta tgagagcgtc ggcaagcatc ttgcaagctt aatacctccg
      421 ggctcttctg tttattggga aggcggccta tctgtagtac ctttactata tttgcccgga
      481 attaggattt atcctgcaca gataaacgat ggctattcgt tccgtattgg ggggaatgca
      541 gaagagctcc aacgcgctgg gttgtggatt tcggaattac aaacgcgatg gttatcagaa
      601 gcggatttta ttctcgttga gcaagatcgt tactccaaag cgatcaacta ttggacagaa
      661 attttaaagc cggatcgatt taatgaatta gaaatttcac cttcctttct tccttgccgt
      721 gaaacgcgct tgcgagtttt ccagagaatt gagcaatgaa aattaattta tcggcatttg
      781 cgaaaaaaca gtctaaaata ctgcgcatcg tcggcacagt gaccgcattg gctttattga
      841 tttatgtgct tcacgagcaa ggctgggaag aaatcgggaa tgccgtaaag cagatcgatg
      901 tatggcggtt ggggctagcc ttgttaatga tgataatttc acggctggct gtgtccggtc
      961 gttggtacat cttattgcgt tcgggagacg ccccaataac tccaaaagag tgttttcaga
     1021 tcacctttgc agggcttttt gctaacaatt tcatgcccac aacggttggc ggagatgtaa
     1081 tccggttggc tggtgcactc cgcttacaat gtgatacagc catcagtgcg gcttcgctca
     1141 tcgccgaccg gttggtcggc atggcaggca tgctgatgat tttaccagtt gggctgccac
     1201 gactcctgca gatccatgat ctccgcgatc tgattcccac aaacgctgaa gtaatccaat
     1261 tcagcttggc tgcaacacca ttgggcgatc tacccagaaa gattttcggg cgtctccaaa
     1321 gcatcttaca ccgcctgctt tcaacgctga cagtatggct gcggcagccg cgcgctctgc
     1381 ttctctctct tctttttaca tggatccatc agatctgcct gtttagcgcg ctgacacttt
     1441 tctttagtgg catgggtgag agcatttctt tctggatggt tggtggtctc tacagctttg
     1501 tttatctgat caccttgatc ccaatctcta taaacggata cggcttacag gaggtttcta
     1561 tgacccttgt tttctcgcgc ctaggaggcg cttcgcttgc cagcagcctg acggctgcac
     1621 tgctgctgcg cacagcagtg atggttgcta gcctgcctgg cgcagctttc gtccccagct
     1681 tgctggcata cagccgcgaa cgccctgaaa caaaaaatgc tccccaaacc gatattgtct
     1741 aacacagaaa gtggatatgg ccatgaaact atcagttgta atccctgtat ataacgaagc
     1801 tgaaaaccta ccagtgttgc aaaaagcgtt aagcaattgc tttgggggtc aagatcgaga
     1861 ctgggaggta atttatgtag acgacgggag cactgatgtc agcctcacta tcctggaagg
     1921 atttgcgtct gaagaacccg agcacacctc tgtggttagc ttgcgtcgca attttggaca
     1981 aaccgctgcc atcgccgccg gcatcgacca tgcgaatgga gacatcatcg tcttgatgga
     2041 cgccgacatg cagaacgacc cggctgacat cccaaagatg ctggagaaaa tcgaggaagg
     2101 ctatgatgtg gtcagcggct ggcgcaagaa ccgccaggac acttttatca cccgtacatt
     2161 accatcccgc attgcaaact ggttgatctc caccatcaca ggtgtgcacc tgcacgatta
     2221 cggctgcacg ctcaaggcct accgccgcga agcgttgact ggtttccgct tgtatggaga
     2281 gatgcaccgc tttatccctg cttatgccca tgcagttgga gccaccatca tcgagctacc
     2341 tgtcacccac catccccgta aatttggcaa aaccaaatat ggactaaacc gcacgattaa
     2401 agtcatcttg gaccttttca cggtcaagtt cctgatcagt tatgcgcaca agcccattta
     2461 tctttttgga gg
//
LOCUS       DBNHOH010000854         1328 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_246825, whole genome shotgun sequence.
ACCESSION   DBNHOH010000854 DBNHOH010000000
VERSION     DBNHOH010000854.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_246825"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="AC16V7_15395"
     CDS             complement(<1..812)
                     /locus_tag="AC16V7_15395"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6258555.1"
                     /translation="MIKLILSANTDWYLYNYRLALAEAARQRGYQVTFVSPPGPYVAE
                     LQQRGFRWLRWDLERRRTNPLAELPSLLAIAAIYRRERPNLAHHHTVKPVLYGSLATK
                     MLGQPAIVNSITGRGYMYQSQSQGARLLRRLVEPLYRFAFSFPRGAVIFENEADRQYF
                     LKQGLISEDRTWLIPGVGVDPERFTPQPTPPEPVVILLASRMLWDKGVGVLVEAARLL
                     RRHTTARVVLVGTPDPGNPATIDESILTQWNQEGVIEWWGWHADMPSMYNQS"
     gene            complement(830..>1328)
                     /locus_tag="AC16V7_15400"
     CDS             complement(830..>1328)
                     /locus_tag="AC16V7_15400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6258556.1"
                     /translation="EMLEVLKHHPEWTLDLAGFGGDETRIISTAEKMPNVIWHGKVSY
                     ERAMQLSAAADVLFATYDPAIPNHRYASPNKLFEAMMLAKPIIVCEHTNMNRIVADAD
                     CGVVLPYGDISALENALGRLASDTAFRRRLGQNARHAYESDYSWAKMESRLIELYAGL
                     SSYPK"
BASE COUNT          287 a          367 c          398 g          276 t
ORIGIN      
        1 tgactctgat tgtacatcga aggcatgtcc gcgtgccagc cccaccattc aataactccc
       61 tcttgattcc attgagtaag gatggattcg tctatcgtgg ctggatttcc agggtccggc
      121 gtgcccacca ggaccacgcg ggctgttgta tgacggcgca gaaggcgagc cgcctccacc
      181 agcacgccaa ctcctttatc ccacagcata cgactcgcca gcaagatcac cactggctct
      241 gggggggtgg gttggggggt gaagcgctca gggtcaaccc ccacaccggg gatcagccag
      301 gtgcggtctt ccgatatgag cccctgcttt agaaagtact gccggtcggc ctcgttttca
      361 aagatcactg cgccgcgcgg aaaggaaaag gcaaaccgat ataatggctc aacgaggcga
      421 cggagcaggc gcgcgccttg actttgagac tgatacatat aacctcgccc ggtgatggaa
      481 ttgacgatgg cgggctgtcc aagcatcttg gtcgccaaag agccatacag caccggtttg
      541 actgtatggt gatgggcaag gttggggcgc tcgcgccggt agatggctgc aatggccaat
      601 agcgagggca gctctgccag cgggttggtg cgtcgtcgct ccagatccca gcgcagccaa
      661 cggaagccgc gctgctgtag ctccgctacg tatggcccag gcggcgagac aaaggtaacc
      721 tgatatccac gctgacgggc ggcctcggcc aaggccagac gataattata caggtaccag
      781 tctgtgttag cagacaggat cagctttatc attttgggat ctaaaagggc tattttggat
      841 acgaactgag acccgcgtaa agttcaatca agcgggattc catcttggcc cagctatagt
      901 cgctctcata agcgtggcgt gcgttctgtc ccagccgacg gcggaaggcc gtgtcggagg
      961 cgaggcgacc aagggcattc tccagggcgg agatatcccc ataaggcagc acgaccccgc
     1021 aatcagcgtc agccacgata cgattcatat tggtgtgctc gcatacgatg atgggcttgg
     1081 ccaacatcat agcctcgaag agcttgtttg ggctggcgta gcggtgattt gggatggccg
     1141 ggtcgtaggt ggcgaagagc acgtcagcgg cagcgctgag ctgcatagct cgctcatagg
     1201 agaccttgcc atgccaaatg acgtttggca tcttttctgc ggtagagatg atacgcgttt
     1261 cgtcgccccc aaaaccagct aggtctagcg tccattctgg gtgatgcttc agcacttcca
     1321 gcatctcc
//
LOCUS       DBNHOH010000855         1823 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_695953, whole genome shotgun sequence.
ACCESSION   DBNHOH010000855 DBNHOH010000000
VERSION     DBNHOH010000855.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_695953"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="AC16V7_15405"
     CDS             complement(<1..746)
                     /locus_tag="AC16V7_15405"
                     /inference="COORDINATES: protein motif:HMM:NF012415.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HIR6258557.1"
                     /translation="MMRKHPKRYHTIVQISLLLLWLLTAPAYQAKNQAQSIAASRSEL
                     ILAINTLRVSQGLPALIEDPIINAVAQSTAEIMAANNMSWHIGDVRGRIAAAGYGGGA
                     TVWATENFAVGNKSVDEIMQVWADPDHMRPVVNPAYCHVGAGVAQAADGRYYYVLQAA
                     YTSDKACGEYKPVPGIDSGSGAKPELPVSQIIIPVKIATPDADGRIYHVVQAGQSFWS
                     IAIAYQITIRDLEVWNNLSRDKGLAMGQKL"
     gene            complement(901..>1823)
                     /locus_tag="AC16V7_15410"
     CDS             complement(901..>1823)
                     /locus_tag="AC16V7_15410"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR6258558.1"
                     /translation="FEVGREITIDYLRDLEISASELVFEEKLADRSNYHQHLVSYISE
                     GNKIYGLLTIPFVEPPEGGFKAIVFNHGYIPPAAYRTTERYVAYVDYLARSGFVVLKI
                     DYRGHGESGGEPSGSYFSPGYTIDSIAALKSLQTLDIIDPQGIGMWGHSMAGNLVLRA
                     MLIEPDIKAGVIWAGAVYSYDDFGKYGIDDNTYRPPVSPGTQGASGRRRGSREIFDAY
                     GRPDTQIDYWKAVSLTENIEYLNSPLQLHHAQDDTVVNFGYSFDLAVVLQDHGKEYEF
                     YTYEGGGHNLISPYFDQAMLRTVEFFRKYL"
BASE COUNT          393 a          496 c          496 g          438 t
ORIGIN      
        1 aataactttt gacccatagc caacccttta tctcttgaga ggttgttcca aacctccaga
       61 tcgcggatgg taatttgata tgcgatggcg atagaccaga atgattgccc cgcctggacg
      121 acgtgataga tcctgccatc ggcgtcgggt gtggcgatct tcaccgggat gataatctgc
      181 gaaaccggga gttctggctt agcaccagat ccggaatcga tcccaggcac gggtttgtat
      241 tcaccgcaag ccttgtcgga ggtgtatgca gcctgcaaaa cgtagtaata gcgaccatcc
      301 gcagcctggg ctacgcccgc gcccacatga cagtatgccg gattgaccac tgggcgcatg
      361 tgatcgggat cagcccagac ttgcatgatc tcatcaacgc tcttgtttcc cacggcaaag
      421 ttttcggtag cccaaactgt cgcgccaccg ccatagcctg cggcggcgat gcgaccgcgc
      481 acgtccccaa tatgccagga catgttgttg gctgccatga tctcggcagt cgattgcgcg
      541 acggcgttga taatcgggtc ttcaatcaaa gcaggcaaac cttgcgagac gcgcagggtg
      601 tttatagcca ggatcaattc actgcgggat gctgcaatcg attgggcttg atttttcgcc
      661 tggtacgctg gcgcggtcag caaccaaagg agcagcaggc taatctggac tatggtgtga
      721 tatcgttttg gatgctttct catcataagc tgatacttta ttggctggcc tggagcatcg
      781 gtggtgaggg gggttgcgct gcgagctcga ttaagtgtat caccgggtat ccttttagaa
      841 catgccgatc accatccccg gttgggcagg gcattcttcc accatcagct gatcatgtga
      901 tcacaagtat tttcggaaga actccaccgt acgtagcata gcctggtcga aatagggtga
      961 gatgaggttg tgtcccccgc cttcataggt gtaaaactcg tattccttgc catgatcctg
     1021 cagcacaaca gccaggtcga acgagtagcc aaaatttacc actgtgtcgt cctgcgcatg
     1081 gtggagctgt aatgggctgt tgaggtattc gatattttcc gtcagggaga cagccttcca
     1141 ataatcaatt tgggtgtccg gccgcccgta cgcatcgaat atctctcgcg atcccctgcg
     1201 gcgaccgctg gctccctgag tccccggcga cactggcggc ctgtaggtat tgtcgtctat
     1261 tccgtatttt ccgaaatcat catagctgta gaccgcccct gcccatatca ccccagcttt
     1321 aatatccggt tcgatgagca tcgcccgtaa aaccaggttg ccggccatgc tgtgacccca
     1381 catcccgatg ccttggggat cgatgatgtc cagcgtctgt aggctcttga gcgccgcgat
     1441 cgagtcgatg gtgtacccgg gcgagaagta tgacccggat ggctctcctc ccgactcccc
     1501 gtgcccccgg taatcgatct tgagaaccac aaaaccgctc cttgccaggt aatcaacgta
     1561 ggcgacgtaa cgttctgtgg tgcggtaagc ggctgggggt atatacccgt ggttgaatac
     1621 gatcgcctta aacccgcctt cagggggttc cacgaagggg atggtcagca ggccatagat
     1681 cttgttgcct tccgagatat agctaaccag gtgctggtgg taattggagc ggtcggcgag
     1741 cttttcctca aaaactaact cactagcgga gatctccaag tcacgcagat agtcgatagt
     1801 gatctctctg ccgacttcga aga
//
LOCUS       DBNHOH010000856         8854 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1272466, whole genome shotgun sequence.
ACCESSION   DBNHOH010000856 DBNHOH010000000
VERSION     DBNHOH010000856.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8854)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8854)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8854
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1272466"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..2196)
                     /locus_tag="AC16V7_15415"
     CDS             complement(193..2196)
                     /locus_tag="AC16V7_15415"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR6258559.1"
                     /translation="MYNLLVIKKPMTMMLTLVIMLSVVAPVMAQENRDTPGKNVSTTP
                     SQGQGPDDPAELEAFLDELMAKDMEEHHIPGAAVSVVKDGMLFFAKGYGYADLENRIP
                     VEPEQTVFAIGSAGKLFTWTAVMQLVEQDKLDLEADVNTYLDFHIPDTYPQPITLRHL
                     LTHTAGFEDLWFEAFAWEADDLMPVGEWLASHIPGRVRPPGEVAAYSNYGTMLAGYIV
                     ARVSGQPYEQYVQEHIFDPLRMEHSTMQRPLPPNLREHESKGYWYSDGAYQAVPVVPD
                     DYLGQMAMNPAGGHLSSAMDMARFMITHLQGGFYGDASSEIRILEETTARQMQDTLYA
                     PDPRILGTAYGFFDFSDNGQRTIGHSGSTIGFATLLLLLPDQNLGVFVVYTNEDVGGL
                     TTQHFGFQRAFFDHYFPAPSVEPIQPPADFDQRASRFVGSYRNARSAYTTFEKFFNLM
                     RPPVEISDPGDGTLLLTINGLELRLVEVEPLYFRQVDGPFGVVFREDDRGRITHMFLD
                     VSPQEDYEKMNWYETAGFNMALLLGCVLAFLSMMPVAVVRAIRNRRPSDDRKPASRGA
                     RIANWTIVGISVLNLLFVTGTVMMASGLGFPRFGLTLTDRIVLGLGVFSALLTVGALV
                     FTILAWKNRYWGNAARTYYTLVTVAAVVFVWFLNQWNLLGWRY"
     gene            complement(2231..3049)
                     /locus_tag="AC16V7_15420"
     CDS             complement(2231..3049)
                     /locus_tag="AC16V7_15420"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6258560.1"
                     /translation="MSANVSGQFQLVNEKGWRRGLGNLLQGEYSSWFKSSRWWKHLIL
                     WFSIINVMMVIMIIASAQAAKGGDEGPPILFMYGIFGGMFVAFGVMIIMQRVLVGEKR
                     SGTAAWVLSKPVTRTAFVVSRLVANSIAILLTSVIVPGVIYYITLGLFSDFGWLSPFG
                     FTAALLMISLHTFFWIALVLMMGTLSESSGGVIAVPMALYFIFWMGTGMVPGLIYISP
                     LLLTFSPDPEQMNALSVSFMTGEPVFSWLPLISTAVYCVIFIAVAIWRFNRQEF"
     gene            complement(3046..3999)
                     /locus_tag="AC16V7_15425"
     CDS             complement(3046..3999)
                     /locus_tag="AC16V7_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015121894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6258561.1"
                     /translation="MNSNDLVISTEGLSKSFGEVHALNSLNLRVPQKSIFAFLGPNGA
                     GKTTTIKLLLGLLKPTSGGGKIFGMDILHDSVDIRANIGYLPQDTRFYDHMTARQTLE
                     YTAKFFYAGPQSEIDKRVNEMIELVGLEGKADRPIKGFSGGERQRLGIAQAEVNYPDL
                     LILDEPASSLDPQGRRDVLDVMSRIRKYATIFYCTHILDDVQRVSDQVAIVNQGELVT
                     QAPIEELLAGTGDAIYSVTLRGDAHSAYTQVNQQAWVSGIEASQQGEQTTWQVSVTDE
                     AAAKDQLMSLLVSNGLKVSNFSRKEHNLEDIFINIVERSQK"
     gene            complement(4213..5025)
                     /locus_tag="AC16V7_15430"
     CDS             complement(4213..5025)
                     /locus_tag="AC16V7_15430"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR6258562.1"
                     /translation="MKTALTERNFQATAAVEVGEQYSLAKILGIWASVALPMGLIHWV
                     VSPILIPRVNMVPGFLYFIMITLGLVWQGVVAYLILRREVKPFTWENLKDRLWLHTPT
                     DPNTGVPSKRLYLWTIPLIVVINVGYAISGRLNELWVKILPFLTPPPYTEIQNLAEPA
                     VGQWWLLGVVAVMIVFNYLVGEELIFRGILLPKMNGVFGKWDFIANAILFATYHLHKI
                     WVWPQILVFNWIIPWAAKRFKSYWVAAIIHGAFDALTLIILFTMAIMGLLSG"
     gene            complement(5039..5857)
                     /locus_tag="AC16V7_15435"
     CDS             complement(5039..5857)
                     /locus_tag="AC16V7_15435"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR6258563.1"
                     /translation="MSTQEITSKDKSRVLSTHHEKSQYNLSQILWIWALASIPGALLF
                     WLGLPVLDRITDISVGYLVLIVIVTPYFWHFLLAFLILKKEGGDLDWNTIKDRLWLRT
                     TADPRTGKRNNTLWFWVIPAIAVYGITAALPFFQSINDGWARILPIHEPAQYSFDVLF
                     ENPGALVGDWGFFLAILMVSLLTVSEEIIFRGILLPKMNGVFGRADWIANGFLFAFYH
                     LEQPWTWPSKILYSTLTLALPARLFRSTWFSTAVHLSQGFYFLFLTLGLVLGLA"
     gene            complement(6005..7111)
                     /locus_tag="AC16V7_15440"
     CDS             complement(6005..7111)
                     /locus_tag="AC16V7_15440"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6258564.1"
                     /translation="MMKTIFKRTIFSIFALLAIVLGSTVIKIWPENYFWVERNNARMP
                     VWVRGNVASGIFLVLNHGGPGSSGTAESLFEINPPNGQFDHASPLQALENEYAVVYWD
                     QRHSGMSSGNADPNDSQIEDFGEDLSLVIRELRARYHVQHLFLIGQSWGHSVALSYLT
                     LIDGWAGNQAGVDGYIIYKANNEQDIAYQAARPRIIQFANQEIAAGRDIAYWKAAIQF
                     YEQRPHLRDVTDFSANSEYADRAMQVSYPLQARIWTAFKSSIFSPLNGWAIYFNFKKT
                     IEAEGFIRYVTTDTTLRQTLPHLSIPVLLIYGAKDLQAPVEVGQSIYQDISTPESQKT
                     LLILPNSRHGAEGNDVPLMQTAIKDFINQTLTLK"
     gene            complement(7124..7810)
                     /locus_tag="AC16V7_15445"
     CDS             complement(7124..7810)
                     /locus_tag="AC16V7_15445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258565.1"
                     /translation="MKSNTSTSQKIGWVVFTIGGLYMFGLGWLYSWQMVPAANQIGSD
                     AYSGLLGFIWALSVPLGAFIVAVGAALIARVERRVFWLLILLVVLFTVWRIAGTTNHM
                     IPALFGIGGGLITLFFLGSTWHWARIRPTLTGAGKTGSDLRMVGFIFFVVAAWDLCGI
                     FGMVNYVLRPELADKFSVPIGSTINAASTVMVLLILGWGFTFFGQLMSRRAQAVDPRV
                     DQVLQMAATD"
     gene            complement(7944..8573)
                     /locus_tag="AC16V7_15450"
     CDS             complement(7944..8573)
                     /locus_tag="AC16V7_15450"
                     /inference="COORDINATES: protein motif:HMM:NF021443.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HIR6258566.1"
                     /translation="MKTESQSVELGDAKPVNVEINFGAGDLRVNGGAEKLLEANFTYN
                     VARLKPEVESTDDTLIVRLPEDKGLPAFGDIADFRNEWDLRLNNEVPMDLSVNVGAGT
                     SDLKLAGLSLTGLDIHLGASESTVDLSGNWVRDLDITIEAGVGDVTVRLPKDIGVRVE
                     VETAIGTMDAPGLKQDGNVYTNAAYGVSDVTLHIKLEAGIGQINLEVTD"
BASE COUNT         2195 a         2339 c         2233 g         2087 t
ORIGIN      
        1 catcggggtg ccggtcataa aagaaacaga cagggcgtgc attacggact tccaaatcat
       61 ttgaattcat acggttttct cctttccatc aaataagact gttgctatct gattataagg
      121 gatttatata aaaaaggaaa cacatgatca tgtgtttcca ggaggggaaa atatgtagtt
      181 atgccgttca atttagtatc gccaacccag taggttccat tggttcagga accagacaaa
      241 aaccaccgcc gccaccgtca ccagtgtgta ataggtgcgg gctgcgttgc cccagtagcg
      301 gttcttccag gccagtatag tgaagaccag cgcgccgacc gtcagcaggg ctgaaaaaac
      361 gcccagtcct aacacgatcc tgtcggtcaa tgtgaggccg aaccttggga atcccagacc
      421 gctcgccatc atcactgttc cagtaacgaa cagcaggttc aaaacgctga tccccacgat
      481 cgtccaattg gcgatgcggg cgccgcgaga ggcgggtttc cgatcatcgc tggggcggcg
      541 gttccgaatc gcacggacca ctgcgacggg catcatggac aggaaggcca agacacatcc
      601 gaggagcaat gccatattga aaccggcggt ttcgtaccag ttcatttttt cgtaatcctc
      661 ctggggcgag acgtcgagaa acatgtgggt gatccgaccc cggtcgtcct cacgaaaaac
      721 aacaccgaat ggaccgtcta cctggcggaa gtacaacggt tccacttcaa ctaaccgcaa
      781 ctcgagccca tttatggtca atagcagtgt gccgtcaccg ggatcgctaa tttcaacggg
      841 tggtctcatg aggttaaaga atttttcgaa cgttgtatat gcgctccgag cgtttctata
      901 tgaacccacg aaccggctgg cacgctgatc gaaatccgcg ggaggttgga ttggctcaac
      961 agagggtgcg ggaaagtaat ggtcgaaaaa cgctctctgg aacccgaaat gctgcgtagt
     1021 cagtccgcca acatcctcgt tggtatagac cacaaagaca cccaggttct ggtcgggtaa
     1081 aagcagcaac agggttgcga aaccgatcgt gctgccgcta tgcccgatcg tgcgctggcc
     1141 gttgtcgctg aaatcgaaaa agccgtaagc ggtgcccagg atgcgcgggt cgggagcgta
     1201 caatgtgtcc tgcatttgcc gcgccgtggt ttcctccagg atgcgtatct cggaggatgc
     1261 gtcgccgtag aagccgccct gcaggtgggt gatcatgaaa cgcgccatgt ccatggcact
     1321 gctcaaatgt ccgccagcgg gattcatcgc catctgaccc aggtagtcat cggggaccac
     1381 agggactgct tgataggcgc catcggaata ccagtatccc ttggactcat gctcgcgcag
     1441 gtttggcggt aacggcctct gcatcgtcga atgctccatg cgcagcgggt caaagatatg
     1501 ttcctggaca tactgctcat agggctgtcc tgagacccgc gccacgatat accccgccag
     1561 cattgtgccg taattcgaat atgccgcgac ctcgccaggt gggcgtacac gtccaggtat
     1621 gtgggaagcc agccattcgc ctaccggcat caggtcatca gcttcccacg caaaagcctc
     1681 aaaccacagg tcttcgaaac ccgccgtatg ggtcaaaaga tgcctgagcg tgatcggttg
     1741 tgggtatgta tctggaatgt gaaagtcgag ataggtgtta acatccgcct ccaggtccag
     1801 cttgtcctgc tcgacgagct gcatcactgc cgtccaggtg aatagcttgc cggctgaacc
     1861 aatggcaaag actgtctgtt cgggctcgac agggatgcgg ttttccaggt cggcgtagcc
     1921 gtagcctttg gcaaaaaaca gcatcccgtc tttgacgaca gacacggcgg ctcccgggat
     1981 gtggtgctct tccatatcct ttgccatgag ctcatccagg aatgcctcca gttcggcggg
     2041 gtcgtcaggg ccttgtcctt gggagggcgt ggtagataca ttcttacccg gcgtgtccct
     2101 gttttcttgg gccatgactg gtgcgacaac cgacagcatt atcacgagag ttaacatcat
     2161 cgtcatgggt ttcttgatga cgagcaggtt gtacattttc cttctcctat acgaatattt
     2221 cgcaagatca ttaaaactcc tggcggttga agcgccagat ggctacggcg ataaaaatga
     2281 cgcaatagac tgccgtcgag atcaacggca gccatgagaa caccggctca ccggtcataa
     2341 aagaaacaga cagggcattc atttgttctg gatcagggct aaaggtcagc agcaatggcg
     2401 agatatagat caggccgggg accatacccg tccccatcca gaatataaag tacagggcca
     2461 tcggtacggc gataacaccc cccgatgatt cagaaagggt tcccatcatc agtaccaatg
     2521 cgatccaaaa gaatgtgtgg agtgaaatca tcaatagggc ggcagtaaat ccgaagggag
     2581 aaagccaccc aaagtcacta aataatccca gggtgatata gtagatcacg ccaggcacga
     2641 tcaccgaggt gagcaggatg gcgatcgaat tggcaaccaa ccgggagacg acaaaagccg
     2701 tgcgcgtgac cggtttggag agcacccagg ccgccgtgcc ggagcgcttt tcaccgacca
     2761 ggacccgctg catgatgatc atcaccccga aggcgacaaa catcccgcca aagatgccat
     2821 acatgaatag gattggcggg ccctcatccc cacctttggc tgcttgggca gacgcaatga
     2881 tcatgatgac catcatcacg ttgatgatcg aaaaccacag gatgagatgt ttccaccatc
     2941 tggaggattt gaaccatgac gaatactcgc cctgcaacaa gttacccaac ccccgcctcc
     3001 agcctttttc attgacaagt tggaactgcc ccgacacatt ggcgctcatt tctggctcct
     3061 ttcaacgata ttgatgaaga tatcttccag gttgtgttct ttgcggctga agttggagac
     3121 cttcaaaccg ttggatacca gcaggctcat taactggtct ttagccgcgg cttcgtccgt
     3181 cacgctcacc tgccaggtgg tctgttcgcc ctgttggctg gcctcgatcc cggaaaccca
     3241 ggcttgctgg ttgacctggg tgtaggcgct gtgggcatcg cctctcaggg ttacggagta
     3301 gatcgcatca cctgtacctg ccagcagttc ttcgataggt gcctgggtga ccagttcacc
     3361 ctggttgacg atcgcaacct ggtcgctgac cctttgtacg tcgtccagga tatgggtgca
     3421 gtagaagatc gtggcgtatt tgcggatgcg ggacatcaca tcgagcacgt ctcgccgtcc
     3481 ctgcggatcg aggcttgagg caggttcatc caggatcaat aggtccggat agttgacctc
     3541 cgcctgggcg atgcccaacc gctggcgctc acctccggag aaacccttga tcggccggtc
     3601 tgccttgccc tccagtccaa ccagttcgat catctcattc acgcgcttgt cgatctccga
     3661 ttgaggtccg gcatagaaga acttagcagt gtattccaga gtctggcggg cggtcatgtg
     3721 atcgtagaaa cgggtatctt gaggtaggta gccgatgttg gcgcggatat cgacgctatc
     3781 gtggaggata tccatgccaa agattttgcc gcccccgctg gtaggtttga gcagccccag
     3841 caacagcttg atcgtggtgg ttttgccagc accgttaggc cccaggaagg cgaagatcga
     3901 tttctgaggg acacgcaggt tgagcgaatt gagcgcatgc acctcgccaa aagacttgct
     3961 caaaccttcc gtgctgatga ctaagtcatt tgagttcatg ttgctttctc cttttcttca
     4021 agtgagacta tctcgattac attctataac ccaagcaaaa aaaaagaacc acatgatcat
     4081 gtggttctaa gacaggtaaa tatgtggttt tgccgccagg tgcagatcaa attcatctct
     4141 cggtcatgac gccgccgcac catatggttg atcctccact cccaggtcgc cagctgcatt
     4201 agccgatcaa tcttaaccgc tcaacaatcc catgattgcc atagtgaaaa ggatgatcag
     4261 tgttaaagcg tcaaacgccc cgtggatgat ggccgccacc cagtacgact tgaaccgttt
     4321 tgccgcccag ggtatgatcc aattgaagac gaggatttgc ggccataccc agatcttatg
     4381 gaggtggtat gtcgcgaaca gaatggcgtt ggcgataaag tcccacttcc cgaacacgcc
     4441 gttcattttt ggcaacaaga ttccccggaa gatcaattcc tctcccacca ggtaattgaa
     4501 cacgatcatc accgcgacga cacccaacaa ccaccattgt cccacggctg gctcagccag
     4561 attctggatc tccgtataag gaggaggcgt caaaaatgga aggatcttca cccagagttc
     4621 gttcaacctc ccagagattg catatcccac attaataacc acgatcaatg ggatcgtcca
     4681 gaggtacagc ctttttgatg gcacgcctgt gttgggatcg gtgggggtat gtagccacaa
     4741 cctgtctttg aggttttccc aggtgaatgg tttcacttcc cgcctgagga tgaggtaagc
     4801 aacaaccccc tgccagacca aacctaatgt aatcataatg aagtacagga atcccggaac
     4861 catgttcaca cgtgggatca ggattggact gaccacccaa tggatgagtc ccatgggcag
     4921 ggcaacgctt gcccagatgc ccaggatttt ggcgagagaa tactgttcac ctacctcaac
     4981 ggctgcagtt gcttggaaat ttctttctgt gagtgctgtt ttcatgtttt ctccttattt
     5041 acgcaagacc caatacaagc cccaaggtta gaaaaagaaa gtaaaatccc tggctaagat
     5101 gcacagcggt cgagaaccag gtgctccgga ataatcgagc cggcagggcc aaagtcaggg
     5161 tggaatacaa aattttgctt ggccaagtcc agggctgttc caaatgatag aaagcaaata
     5221 ggaagccgtt ggcaatccaa tctgctcggc caaatactcc gttcattttc ggaagcaaaa
     5281 tcccccgaaa tatgatctct tccgacaccg tcaatagact gaccataaga atggcgagga
     5341 aaaatcccca atcaccgacg agcgcccccg gattttcaaa cagcacatca aagctgtatt
     5401 gtgcaggttc gtggatcggt agtattcgtg cccacccatc attgatggat tgaaagaatg
     5461 ggagtgcagc cgtgatccca taaaccgcga tcgctggaat cacccaaaac cagagtgtgt
     5521 tgtttcgttt tccagttctt ggatcggccg tcgttctcag ccataatcga tccttgatgg
     5581 tattccaatc caggtcacca ccttccttct tcaggatcaa aaaggccagc aaaaagtgcc
     5641 agaagtatgg ggtgacaatg acgatcagca ccagatagcc gacactgatg tcagtaattc
     5701 gatccaggac cggtaaaccc agccaaaaaa gcagggcacc aggaattgaa gcaagcgccc
     5761 atatccatag gatctgtgaa aggttgtact gggatttctc gtggtgtgta gaaagcactc
     5821 gcgatttatc ctttgatgtt atttcttgag tagacatgag atttctcttt ttttactttt
     5881 aggttatctg aacaaaggtt gtccgttcgt ttatctgata tttccactag atatctcctt
     5941 tgggattaaa acccctggcg gttgaagcgc cagatagcta caaagatgaa aaagaagcga
     6001 tgttttactt caacgtcaaa gtttgattaa taaagtcctt gatagctgtc tgcattaacg
     6061 gaacatcatt gccttcggcg ccatgccggg aattgggcag tatcaggaga gttttttgtg
     6121 actcaggtgt ggagatatcc tgatagatgc tttgcccaac ttctactggc gcttggaggt
     6181 cttttgcacc ataaatcagg agcaccggga ttgataaatg tggtagtgtt tggcgcagag
     6241 tcgtgtcggt ggttacgtat ctaatgaatc cttcggcttc gatggttttt ttgaagttga
     6301 agtagattgc ccaaccatta aggggtgaga agatagatga tttaaaagcg gtccaaatac
     6361 gcgcttgaag tggataagac acttgcatag cccgatcagc gtattcgctg ttggcgctaa
     6421 aatcggtgac atcacgcaga tggggtcttt gctcataaaa ttgaatggca gccttccaat
     6481 aggctatgtc acgcccagcg gcaatttcct ggttggcaaa ctggatgatc cgtggtcggg
     6541 cagcctgata cgcgatgtct tgttcattgt tggctttgta aataatatag ccgtctactc
     6601 cagcctgatt gcctgcccaa ccatcgatta gcgtcaggta actcaatgca acactatgtc
     6661 cccagctctg gccgatcagg aaaagatgct gcacatgata acgtgctctc agttcgcgaa
     6721 tgaccaggga cagatcttcg ccaaaatcct cgatctggct gtcattggga tctgcattcc
     6781 cgctcgacat ccctgaatgg cgctgatccc aatacaccac ggcgtactcg ttctcgagcg
     6841 cttgcagggg agaggcatgg tcaaactggc cattgggagg gttaatttcg aaaagggatt
     6901 ctgctgtgcc ggaactacct gggccaccat gatttaatac gagaaagatg cccgaggcta
     6961 catttcccct gacccatacc ggcatgcggg cgttattacg ctcgacccag aaatagtttt
     7021 ccggccagat cttaattaca gtcgagccta gcacgatagc cagcagtgca aatatcgaga
     7081 agatagttct cttgaaaatt gtcttcatca tgggttattt cctctaatca gtcgcagcca
     7141 tctgaagaac ctgatccact cgaggatcaa ccgcctgtgc ccggcgagac atcagttgac
     7201 cgaagaaggt gaatccccaa cccagaatca gcaagaccat cacagttgat gcggcgttga
     7261 tcgtgctgcc gatcggcaca gagaacttgt ccgccagctc gggtctcaaa acgtaattga
     7321 ccataccaaa tatgccgcac agatcccagg cagccacaac gaagaagata aatccgacca
     7381 tgcgcaagtc cgatcctgtt ttgccagccc ctgttagcgt gggacggatc ctggcccaat
     7441 gccaggtcga cccgaggaaa aacaaggtga tcaagccccc accaatccca aagagcgctg
     7501 gaatcatgtg gttggttgtg ccggctattc tccagaccgt aaaaagcaca accaacagga
     7561 tcagcaacca gaatacacgg cgctcaaccc tggcgatcag ggctgctcct acggcgacga
     7621 tgaatgctcc tagcggtaca gatagagccc agataaagcc caataaccca gaataggcat
     7681 cgctgccaat ctgattggct gcgggcacca tctgccacga atacaaccag cccaagccaa
     7741 acatatacag cccaccgatc gtaaatacca cccatccgat tttttgtgat gtagatgtat
     7801 tgcttttcat gactcactcc tttgacttca aacttacttg tttctctctt gatacgattt
     7861 ttagccgatc tcaacttgat tcactgcacg tgcatttaac tcttgatggc tctatgcagg
     7921 ccaatgccaa gagttgcgcc tgcctaatct gttacttcca ggttgatctg gccgatgccg
     7981 gcctccagtt ttatatgcaa agtcacatct gatacaccat aggcggcgtt ggtgtaaaca
     8041 tttccatcct gtttcaggcc gggggcatcc atcgtgccta tagcagtctc gacctcgacg
     8101 cgaactccga tgtctttagg cagtcgcacg gtgacgtcac ccacgccggc ctcgatggtg
     8161 atatcaagat cacggaccca gtttccgctc aggtcgaccg tgctttcgct cgcaccaaga
     8221 tggatatcga gcccggttaa cgacaggcct gccagtttta aatcgctggt cccggcgcct
     8281 acatttacgc tcagatccat gggcacctca ttgttaaggc gcagatccca ctcattacgg
     8341 aagtctgcga tgtctccaaa ggctggcaga cctttgtctt cgggcagccg cacaatgagc
     8401 gtatcgtccg tggactcaac ctctggcttc agcctggcga cgttataggt gaaattggcc
     8461 tccagcagct tttccgcgcc gccattcaca cgcaggtcgc cagcgccgaa gtttatctcg
     8521 acgttgaccg gcttggcgtc tcccagctcg accgattgag attcagtctt caactcgccc
     8581 acccttagcc tggaactgca gccggcgagt agcagcgcac caaggagcac gagagccagc
     8641 caaacaacgg tgctatactt ttcttttcgt ttattcattt caaaacctcc gttgagtttc
     8701 aatgattgac atttccgact ttgttctaaa gggatctatt tcgttattgg tattctattg
     8761 gcgcagggaa aaaaaagaac cacatgatca tgtggttctc tggttggtaa atatgtggtt
     8821 ttgttcttac taaggaatct caagggttct gacc
//
LOCUS       DBNHOH010000857         3062 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1401220, whole genome shotgun sequence.
ACCESSION   DBNHOH010000857 DBNHOH010000000
VERSION     DBNHOH010000857.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1401220"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="AC16V7_15455"
     CDS             complement(<1..1169)
                     /locus_tag="AC16V7_15455"
                     /inference="COORDINATES: protein motif:HMM:NF024241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6258567.1"
                     /translation="MSTQTTRQSPSILVALALCMALQMTGFIMISPLFPRRFDSFGAG
                     VEALGMSAMIYGLTSTFTAPFIGMSADRFGRRPIILLALAGYVLAFSGFLFATSAWML
                     ITLRGLAGVCTAGLLPAMMSIVGDLAPNNRRAQWIGIVNGGASAGWIGGPLLGGVLYD
                     RFGYVVPFAVSIAMAAGALLLAIFLIHDTHSNAAHLIQARPGWSHQLRALPAGSIFIL
                     LMLITFGVMFAWAFIEPQFMFYVYDDLNWTSSQLGLIMSSYGVAFMIGEFTLGQLSDR
                     LGRKPVLVLGLALFSAQFVGLFTFRDATWIVVSFILAGLGNALYDPALSALILDVTPA
                     EHKAGMMGLKGTAGSLGNMLGPGLVVLFTPFTSPQVVFLMAAVLVVMLALACGLA"
     gene            complement(1242..2288)
                     /locus_tag="AC16V7_15460"
     CDS             complement(1242..2288)
                     /locus_tag="AC16V7_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258568.1"
                     /translation="MVHTKISAVVKALRLRYAIPFVLLLVTVYWTGIHPWMANWGSTA
                     SEQQMALPGDEFNLDRIPQSTLAITINAPSDVVWQWLVQIGQDRAGFYTYTWLENLIG
                     ADIHNANEIHPEWQYLAVGDAWRLVPPDYLWGLGKEAVSPVLISEPGHALVLEMFGAH
                     VIVPINEHTSRLIVRGQSGPSSLVTTMVWEPLVFTMSRRMLLGLKARAEGRRDAPAAL
                     MAIAQLGWVAAGIVVAGLFMKQQRRQYWLVLPVVAALPALLTSSDFQAALAAFIAVGI
                     TVLGFLIFGKSWWGSFLVIGSVVMLTLLLAPEAYTAIGLAFTVLLLATLGATVGSRSS
                     TVDGAAHQLAAPTQ"
     gene            complement(2417..2905)
                     /locus_tag="AC16V7_15465"
     CDS             complement(2417..2905)
                     /locus_tag="AC16V7_15465"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016107.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR6258569.1"
                     /translation="MINIFKMQNGMNIVGQGGKIILFMLPSLIAAILVQAYFPQFAAL
                     PESIGFIKPVGYLLLLLGLILWMTAVIQLMAGFSKGKLVTTGAYGVVRNPIYSSVTFF
                     VLPAVALITLTWVYFVPSIFLYVGVMIFIGTEEKQLTTAFGKEYEDYLARVDRLVPFK
                     KP"
BASE COUNT          757 a          847 c          863 g          595 t
ORIGIN      
        1 aaggccagcc cgcaagcaag cgcgagcata actaccaaga ccgcggccat caggaataca
       61 acctgtggac tggtaaaggg tgtaaacagc accaccaacc ccggtccaag catatttccg
      121 agggaaccgg ctgtgccttt gagtcccatc atacccgcct tgtgttccgc tggggtaacg
      181 tcgaggataa gggcgcttaa agccgggtcg tagagggcat tgcccagccc agccaggatg
      241 aagctcacca caatccaggt cgcatcgcgg aaagtgaata acccaacgaa ctgcgccgag
      301 aacagtgcca gtcccagcac cagtacaggc ttacgcccca ggcggtcgct gagttggcca
      361 agggtaaatt cgccgatcat gaaagccact ccataggagc tcatgattag ccccagttgt
      421 gaggatgtcc agttcagatc atcgtaaaca tagaacatga attgcggctc gataaatgcc
      481 caggcgaaca tcacaccaaa ggtgatgagc atgagcaaaa taaatattga cccggcaggc
      541 agcgctcgca gctgatgcga ccagcccggt cgcgcctgaa tgagatgggc ggcatttgag
      601 tgcgtgtcgt ggatcaggaa tattgctaat agtagcgcgc cagcagccat cgcaatcgat
      661 acagcaaatg gcacgacata accgaaacgg tcatacagta ccccacccaa taacggaccg
      721 ccgatccatc cggctgaggc accgccgttg acaatgccaa tccattgggc gcgccggtta
      781 ttcggtgcca ggtcgccgac aatgctcatc atggccggca gcaggccggc agtacagaca
      841 cctgccaggc cgcgcagggt aattagcatc caggcggagg tagcgaacag aaaaccgctg
      901 aatgccagga cataaccagc gagagcaagt aggatgatgg gacggcgtcc aaagcggtcg
      961 gctgacatcc caatgaaggg cgcggtgaag gtgctggtaa gaccatagat catggcactc
     1021 atcccgaggg cttccacgcc agccccaaaa ctatcgaagc ggcggggaaa cagcggcgag
     1081 atcataataa agccggtcat ttgcaaggcc atgcaaagcg ccagggcaac cagaatgctt
     1141 ggagactgcc gggtagtctg ggtactcatt aatcggtcct ctcgatgacg cttgttctgt
     1201 aaggcagcgc gctggtgggc tcggactgtg tgcaagtcat gctattgagt tggcgcagct
     1261 aactggtgtg ccgcaccgtc cacggtgctg gagcgactgc cgactgtcgc acctagggtc
     1321 gccaggagaa gcacggtgaa cgcaaggcca atagcagtat aggcctctgg cgcaagcagc
     1381 agtgtcagca tcacaaccga cccgatgaca aggaaggatc cccaccagct tttgccaaag
     1441 atcagaaacc ctagcactgt gatgccgacg gcgatgaacg ctgctagtgc agcctggaag
     1501 tcactgcttg tcagcagcgc aggtagcgcc gccacgactg ggagcaccag ccagtactgg
     1561 cggcgttgct gtttcataaa cagaccggca acgacgatcc cggcggccac ccaaccaagc
     1621 tgggcgattg ccatgagtgc agcgggagca tcccggcggc cctcagcgcg ggctttgagt
     1681 ccaagcagca tgcgccgact catggtgaag accaggggct cccataccat cgttgtcacc
     1741 aggctagatg gtcccgattg accgcgcacg atcagccgcg aggtgtgctc gttgatcggc
     1801 acgatgacat gggctccaaa catctcgaga acaagcgcat ggcccggctc gctgatcagc
     1861 actggactca ccgcctcttt tcccagaccc cagaggtaat cgggtggaac cagccgccaa
     1921 gcatccccga cagccagata ttgccactcc gggtgaatct cgttagcatt gtgtatatca
     1981 gccccgatga ggttctcgag ccaggtgtac gtgtagaagc cggcacggtc ctggccgatc
     2041 tggaccagcc actgccagac tacgtctgac ggcgcgttga tagtgattgc cagggtcgat
     2101 tgtgggatgc gatccagatt gaattcgtcg ccgggtagag ccatctgctg ttcagatgcg
     2161 gtggaacccc agttcgccat ccatggatga atgccggtcc aatagacagt taccagcaac
     2221 aaaacgaacg gaatggcgta gcgcagacgt agtgccttta caacagcaga gatcttcgtg
     2281 tgtaccatgg tgatctcctc tcaagatgtg cgggagtgcg aacttttacc caaattggct
     2341 ggctgaccga gctccgtgat cgtttggggt cttttagcag tgtgccagcc ggtgtgccag
     2401 cgccacagcc gcgccttcaa ggttttttaa atggaaccag tcgatcgacc cttgctaaat
     2461 aatcctcata ctccttgccg aaggccgtcg tgagttgctt ctcctcagtg ccgatgaaaa
     2521 tcatcacgcc gacatacagg aagatcgaag gcacaaaata aacccaggtt agcgtgatca
     2581 gcgcgacggc gggaaggaca aagaacgtca cactggagta gattggattg cggacaacgc
     2641 cgtatgcgcc ggtggttact agcttacctt tggagaagcc cgccattaat tggataaccg
     2701 cggtcatcca aaggatcaac ccaagaagga gtaataaata tcccacgggc ttgataaaac
     2761 cgatgctctc gggcaatgct gcgaattggg ggaaataggc ctggaccaat atggctgcaa
     2821 tcaaagacgg cagcataaaa aggatgattt tcccgccctg cccgacgata ttcattccat
     2881 tttgcatctt gaagatgttt atcatgatgc gtctcctgtt tgttggattt tcattgacgg
     2941 ttgacaacct gccacctggg ctctaattgg tgttgacgat cacgttgcct gcgccgcttt
     3001 gaactgtgat ctctacctta ttggcggcac tatcgaagtc ggacgactgg tacgttttgt
     3061 cc
//
LOCUS       DBNHOH010000858         2257 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_439397, whole genome shotgun sequence.
ACCESSION   DBNHOH010000858 DBNHOH010000000
VERSION     DBNHOH010000858.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_439397"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..1106
                     /locus_tag="AC16V7_15470"
     CDS             123..1106
                     /locus_tag="AC16V7_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6258570.1"
                     /translation="MPNVQAFSQGVIDNIEKVIVGKRATVEQAIISLLCQGHLLIEDV
                     PGVGKTMLARSLARSLGCSFSRIQFTPDMLPSDVTGVSIFNQVSREFEFRSGPVMAQI
                     VLADEINRATPKTQAALLEAMEERQVTVDGVTHSLPRPFMVLATQNPIEYEGTFPLPE
                     AQLDRFLMRIRLGYPGVEDEVLVMERQQYRHPLEILEQMVSAEELATMQETVKGVYVA
                     PSIKRYIVTLARHTREHPDVYLGASPRGSLALYRTSQALAAVRGREFVLPDDVKELVV
                     PTFAHRIILGPAARLRDLDDEQIVLEILENVPVPGGDLGESRVFDGSEVRA"
     gene            1103..>2257
                     /locus_tag="AC16V7_15475"
     CDS             1103..>2257
                     /locus_tag="AC16V7_15475"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR6258571.1"
                     /translation="MNFSLRVVLFLLVASLLAGVVSGSNLYYRLAYLWIFLLAGSYLM
                     SRLALRNLSFQRTARSLRAQVGQFFDERFEIYNSSRLPRLWLEVRDETTLPGTRGSQV
                     LSMIGGRESRTYLVRTRLMQRGVFPLGQTILASGDLFGLFPVSHVYPAQDSLLVYPMM
                     VDVHAFPSPMGWLSGGEALRRRTHQITANASGVRDYAPGDPLNRIHWLSTARRNRLIV
                     KEFELDPLAEVWIFVDASRYVQSELPHQPLVLEEREVWRPSVKIPLPPSTEEYAVSIA
                     ASLARFFLQRKRAVGLVYAGRYFSVLSAERGGRQLGKILETLALLRADGKMPLQSLVD
                     AEVRSLPRGSTVILITPSTGNEVILSAELLARRGMRPVAVLINSASFGGSA"
BASE COUNT          512 a          545 c          622 g          578 t
ORIGIN      
        1 tgcttctggc agtattaata ttttcttcac ggtatcgcct ctggaaggtg ttaaactata
       61 gtcagttttg gtttgtcctg aatgtatttc tgctaaagtc atagttctaa tgggagtaaa
      121 agatgccaaa cgtccaagct ttcagccaag gtgtaattga taatattgaa aaagtgattg
      181 ttgggaagcg cgcaactgtg gagcaggcaa tcattagcct gctatgtcaa ggacatctgc
      241 tgattgaaga cgtcccaggt gtcggtaaaa ccatgcttgc gcgtagcctg gcacgctctt
      301 taggatgtag ctttagccgg atacagttta cgcctgatat gcttcccagc gacgtaaccg
      361 gcgtctcgat attcaaccag gttagccggg aattcgaatt tcgctctggg ccagtaatgg
      421 cgcaaattgt gctagcagat gaaattaacc gcgccacacc caagacccag gctgctctat
      481 tagaggctat ggaagaacga caggttaccg tcgatggggt gacgcactcc ctgccacgcc
      541 cattcatggt gctggctaca caaaacccga tcgaatatga ggggacattt cctctccctg
      601 aagcacaatt agaccgattc ttgatgcgaa ttcgcctggg ctatcctgga gttgaagacg
      661 aagtattagt gatggagcgc cagcaatacc gccatccatt ggagattctt gagcaaatgg
      721 tatccgctga agagttggct accatgcagg agaccgtaaa aggggtgtac gtagcgcctt
      781 cgatcaagcg ttatattgtt actttagccc ggcatacccg cgaacatcct gatgtgtatc
      841 taggcgccag cccgcgcggc agcctggctc tgtatcggac atctcaagcg ctggctgcgg
      901 tgcgtgggcg cgaatttgtg ctacctgatg atgtcaagga attagtagta ccgacatttg
      961 ctcatcgaat tatcctgggg cctgccgccc gtctacgcga tctggatgac gagcaaatcg
     1021 tcttggagat cctcgaaaac gtgcctgtac cgggaggcga tttgggagaa tcaagagttt
     1081 ttgatggcag tgaagtgaga gcatgaactt ctcactgcgt gtggtattgt tccttctcgt
     1141 tgccagtttg ttggcggggg ttgtgagtgg ttccaacctg tattaccgcc tagcatatct
     1201 atggattttt ttgctcgcgg gaagctacct catgtcacgc ctggcattga gaaatctgag
     1261 ctttcaacgg actgccaggt ctttgcgtgc ccaagttggg caattctttg atgagcgttt
     1321 cgagatatat aattctagcc ggctacctcg cttatggctg gaggtgcgcg acgagacaac
     1381 cctgcctggc acgcgtggat cccaggtgct cagcatgatt ggagggcggg aaagccgcac
     1441 gtacctggta aggacgcgcc tgatgcagcg cggcgtattc cctcttgggc agacgatact
     1501 ggcttcgggt gatttattcg gccttttccc agtaagccat gtgtacccag cccaagattc
     1561 gcttcttgtt tatccaatga tggtcgatgt gcatgctttt cccagcccga tgggatggtt
     1621 atcaggtggt gaagccttgc gtcgaaggac acaccagatc actgctaacg catccggtgt
     1681 gcgcgattat gcacctggtg accccctgaa ccgcatccac tggttgagca cagcccgccg
     1741 gaaccggttg attgtaaaag aatttgagct agatccgctg gcggaggtgt ggatttttgt
     1801 ggatgcctcc cgctatgtgc aaagcgaact cccacatcaa ccactggtat tggaggaaag
     1861 ggaggtatgg agacccagtg tgaaaatccc tttaccaccc tccactgaag aatatgctgt
     1921 gagcatcgcc gcctcgttgg cgcgcttttt cttgcagcgc aagcgggctg tcggcttagt
     1981 atatgcaggg cgctatttta gcgtgctatc agcagaacgt ggaggtcgac aattgggtaa
     2041 aatcttggag acgctcgcct tattgagagc agacgggaag atgccgctcc aatctcttgt
     2101 agatgcagaa gtgcgcagcc ttccgagagg cagcacagtg atacttatca ccccctcaac
     2161 aggtaatgaa gttattttat cggcagagct tttagccagg cgagggatgc gtccggttgc
     2221 tgtactcatc aactcggcta gctttggcgg atcagca
//
LOCUS       DBNHOH010000859         3936 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_696550, whole genome shotgun sequence.
ACCESSION   DBNHOH010000859 DBNHOH010000000
VERSION     DBNHOH010000859.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_696550"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="AC16V7_15480"
     CDS             complement(<1..189)
                     /locus_tag="AC16V7_15480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258572.1"
                     /translation="MFQPSQRRVVGGYCKPGRGEEIPGKGDMRTGANPAKAGLPAYQS
                     KRLGIATIMYIPSQDGYFE"
     gene            complement(226..1164)
                     /locus_tag="AC16V7_15485"
     CDS             complement(226..1164)
                     /locus_tag="AC16V7_15485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HIR6258573.1"
                     /translation="MRVGQNPAKSIDSVPQPQRVTVALVSYIPFLGGYYAQSLEVLKT
                     CLGSLWEATRTAFDLLVFDNASCTEVRGYLQDAHSQGRIQYLVLSDKNVGKGGAWNFI
                     FQAAPGEIIAYADSDILFRRGWLERSLEILAAFPQAGMVTARPMRTPEAFYTSTLEWA
                     RQQADVQLEQGNFLPWETYLEHVLSLGTSEEQAREWFASRQDIRLTRQGVSAHVSAAH
                     FQFTAPKSILAQFVPFDMDRPMGQVRSLDEQMNASGYLRLTTCDPLVKHLGNRIEAGS
                     IQDGARPAPKLGFLHLPPVKKALLRIYDEIFRLYHT"
     gene            complement(1181..2092)
                     /locus_tag="AC16V7_15490"
     CDS             complement(1181..2092)
                     /locus_tag="AC16V7_15490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258574.1"
                     /translation="MSTIPFLTIFTAPKSFTDPHIAVIQRNAIQSWCHLDPQVDVLLV
                     GDEPGMAEVAAEYGLRQLPDVARNEAGTPLVSSIFSLARHASPAPLLAYLNADILLLP
                     DFVAAARQVSRLAERFLVIGQRWDLDVRELLDFSAGWEARLRQEVTSRGREHMPAGSD
                     YFVFPRDMFMEIPDFAIGRAGWDNWMIFHARQQGWPVIDATPSITVIHQDHDYSHLPG
                     GRPHYEHPESQVNQSLAGGSANLYMVLDSDRQLVGGRLRRPKITLLRILRRAEVWLTP
                     PGSQRRGLYWKLARPFRRLRRKITGSL"
     gene            2227..2874
                     /locus_tag="AC16V7_15495"
     CDS             2227..2874
                     /locus_tag="AC16V7_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258575.1"
                     /translation="MSEKISLKEADRKVFRTTFQDGLWDVLIGCTLLQFPIALFLSPS
                     LGDFWSSAVFLPFWGLVFGVIWLLRKYVVTPRIGVVKFGLVRKTRILKFNVVLLITLT
                     VSLILGLLASLSRASMPGMVYSAVFGLMTLMGFSIAAYFLDFPRLYAYGILIALAPLA
                     GEWLYVNMNVPHHGFQVTFGFISVTCILIGLGMFFRFLRAYPVPTNGVISEEQEG"
     gene            2871..3200
                     /locus_tag="AC16V7_15500"
     CDS             2871..3200
                     /locus_tag="AC16V7_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR6258576.1"
                     /translation="MTDQPTEQEQLHPLADIDQVIHAPARLMVLTYLYVVESADFIFL
                     MRLTGLTWGNLSTHLSKLEETGYVTIEKTFKGKKPHTLIHLTDRGRVAFSQYKKSMQR
                     VLDDLPD"
     gene            complement(3197..>3936)
                     /locus_tag="AC16V7_15505"
     CDS             complement(3197..>3936)
                     /locus_tag="AC16V7_15505"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6258577.1"
                     /translation="MDDAELTVFYEAEYRQLYQGGEGPDAKDLSVQSRRAAALLAFSK
                     DRLSPVKRHLDIGCSAGLLLQHFQGAYNCQSVGIEPGAAYRQYAQNQGLMVFDSLDEV
                     KGQEMERFDLVSMAHVLEHLPDPVGYLEGLRKDLLEPEGWLLVEVPNLYAHDSFEVAH
                     TVSYSAHTLRQTLGKAGFEVTVLEAHGRPRSHLLPIYLTALAHPFEKHTSFQVHPESN
                     VRLKRRWGMSRRRVLTRLFPRQTWLPI"
BASE COUNT          838 a         1109 c         1136 g          853 t
ORIGIN      
        1 ctcgaaatat ccatcctggg acgggatata catgatggtg gcaatgccca gtcttttgga
       61 ttggtaggcc ggcaaaccag ccttggcagg gttggctcct gttctcatat cacccttgcc
      121 cgggatttct tcgccccttc cgggcttgca ataacctcca accacccgtc tttgcgaggg
      181 ttggaacaac gaaacaatct ccccttcgca tcggacgacg atattctatg tgtgatagag
      241 cctgaaaatt tcatcgtaga tacgcaacaa agcctttttg accggcggca ggtggaggaa
      301 gcccagtttg ggcgcggggc gcgccccgtc ctggatactg cccgcctcta tgcggttgcc
      361 caaatgcttg accagcggat cgcaggttgt aaggcgcagg tagccagagg cgttcatctg
      421 ctcgtccaac gagcgcacct gtcccatggg gcgatccata tcgaagggga caaactgggc
      481 caggatggat ttgggtgcag tgaactggaa atgcgccgcg ctgacgtgag cggagacccc
      541 ctgacgggtc aggcggatat cctgacgaga ggcgaaccat tctcgcgcct gttcttcaga
      601 cgtccccagg ctgagcacat gctccaggta ggtttcccaa ggcaagaaat tgccctgctc
      661 caattgcaca tccgcctgct ggcgtgccca ctccagggtg ctggtgtaga aagcctccgg
      721 tgtgcgcatg gggcgcgccg tgaccatgcc ggcttgcgga aaggcagcca ggatctccag
      781 cgagcgctct agccaacccc gtcggaatag gatgtcgctg tcggcatagg caatgatctc
      841 tcccggggcg gcctggaaga taaagttcca tgccccgccc ttccccacgt tcttgtcgga
      901 gagcaccaag tattgtatgc gtccctggga gtgagcatct tgcagatagc cgcgcacctc
      961 ggtgcaggag gcgttatcaa agacaagcaa atcaaacgct gtccgcgttg cctcccacag
     1021 gctgcccagg caagtcttga gcacctccag cgattgggcg taatagccgc ccaggaaggg
     1081 gatatagctc accaacgcta ccgtgacccg ctgcggttgt gggacactgt ctattgactt
     1141 ggcggggttc tgtccaacgc gcacgatacg attttccttc tcataatgaa ccagtgatct
     1201 tgcgcctgag gcggcggaag ggacgggcga gcttccagta caggcctcgg cgctggctgc
     1261 cgggtggtgt gagccaaacc tcagctcgac gcaagatgcg caatagggtt atcttggggc
     1321 ggcgcaaacg tccgcccacg agttgcctgt cgctatccag caccatgtac aaattggcag
     1381 agccgcccgc cagggattgg ttcacctgcg attcggggtg ctcgtaatgc ggacgcccgc
     1441 cgggtagatg actgtaatca tgatcctggt ggatgacagt gattgaaggg gttgcatcaa
     1501 tcaccggcca gccttgctgc ctggcatgga agatcatcca gttgtcccat ccggcgcgcc
     1561 caatggcaaa atcggggatc tccatgaaca tgtcgcgggg gaaaacgaag taatcgctgc
     1621 cagcgggcat gtgctcccgg ccgcgcgagg tgacttcctg acgcagacgc gcctcccagc
     1681 ctgcggagaa atccagcagc tcgcgcacat ccaggtccca gcgctgccca atgaccagga
     1741 agcgctctgc caggcgggag acctggcggg cagccgcgac aaagtcgggt aggagcagaa
     1801 tatcagcatt tagataggcc agcagcggag ccgggctggc atgacgcgcc agggaaaaga
     1861 tcgagctcac caggggggtg ccagcctcat tgcgggctac gtccgggagc tggcgcaaac
     1921 cgtattccgc cgccacctca gccataccgg gctcgtcacc caccaggagc acatccacct
     1981 gtgggtcaag atgacaccag gattggatag cgttacgctg gatgacggcg atgtgtggat
     2041 cggtgaagga tttcggggcg gtgaagatgg tgagaaaggg gatggttgac aagtgtactg
     2101 aagggattag agtgtcggta gggaagcgag gcatccgctg cccttgacaa actacggacc
     2161 taatgatata ttcaaagtac tttgtttatc aaagcacttt gtaatgctag ttcttgggag
     2221 ggtcagatgt cagaaaaaat tagcctgaag gaagcagatc gcaaagtatt cagaaccacc
     2281 ttccaggatg gcttgtggga tgtgctgatc gggtgcactt tattgcaatt ccccattgcc
     2341 ctattcctca gccccagcct gggcgatttt tggagctcgg ctgtgttcct gccgttctgg
     2401 ggtttggtat ttggtgttat ctggctgctc cgtaaatatg ttgtcacacc ccggatcggc
     2461 gtagtgaaat ttggtctcgt gcgtaaaacc aggattctca aattcaatgt tgtactgctc
     2521 attaccttga cggtttcgct gatactgggg ctcctggcgt ccttgagccg ggccagcatg
     2581 ccaggtatgg tgtactcggc tgtatttggc ttgatgacct tgatgggatt cagcattgcg
     2641 gcctacttcc tggatttccc gcgcctgtac gcctatggca ttctgatcgc tcttgcaccc
     2701 cttgcaggtg agtggctata tgtcaacatg aatgttcccc atcacggctt ccaagtaact
     2761 ttcggtttca tctcagtgac ctgtatcctc atcgggttgg gcatgttctt ccgattcctg
     2821 cgggcttatc ccgtccccac aaatggtgtg atctctgagg aacaagaagg atgactgacc
     2881 agccaacgga gcaggaacaa ctacacccac tggctgatat cgatcaggtc atccatgccc
     2941 cggccagatt gatggtgttg acctacctct acgttgtaga gagcgccgac ttcatcttcc
     3001 tgatgcgcct gaccgggctg acctggggaa acctgtctac ccatctatcc aagctggaag
     3061 agactggcta cgttacgatc gagaagactt tcaagggcaa gaagccgcac acacttatcc
     3121 atttgaccga ccgggggcgg gttgccttca gtcagtacaa gaaaagcatg cagcgagtgc
     3181 tggacgacct gccggactaa ataggcagcc aggtctgccg ggggaataaa cgtgtcagca
     3241 cccggcggcg tgacatgccc cacctacgct tcaaacgcac attgctctca ggatgcacct
     3301 ggaatgaggt gtgcttctcg aatggatgtg ccaacgccgt caggtatatc ggcaataggt
     3361 gcgagcgcgg cctgccgtgc gcctctagga cggtgacctc aaaaccggcc ttccccagcg
     3421 tctgtctgag cgtatgggcg ctgtaagaga ccgtgtgggc tacctcgaag ctatcgtgag
     3481 cgtacaggtt ggggacttct accagcaacc agccctctgg ctccaataaa tccttgcgca
     3541 ggccttccag gtagccgacc gggtcaggca ggtgttccag aacatgcgcc atactgacca
     3601 aatcaaagcg ctccatctct tgccccttaa cctcgtccag cgaatcgaaa accatcagac
     3661 cctggttctg ggcgtactgg cggtaggctg cgcctggctc gatcccaacc gactggcagt
     3721 tataagctcc ctggaaatgc tgcaatagca agcctgctga gcaaccgata tccaggtggc
     3781 gtttcacagg tgatagtcta tctttactaa aggctagcaa ggccgcggct cgcctgctct
     3841 gtacggataa atccttggcg tccggcccct cgcctccctg gtagagctgg cggtactccg
     3901 cctcataaaa gaccgtcagt tcggcatcat ccatgc
//
LOCUS       DBNHOH010000860         1265 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_632228, whole genome shotgun sequence.
ACCESSION   DBNHOH010000860 DBNHOH010000000
VERSION     DBNHOH010000860.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_632228"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="AC16V7_15510"
     CDS             <1..464
                     /locus_tag="AC16V7_15510"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HIR6258578.1"
                     /translation="PCTPDGVIYLLGQVGVELEGCNAVVLGRSNIVGMPVALLLVERN
                     ATVTICHSRTRNLADVCRSADVLVAAVGRPEMVRGGWVKPGAVVIDVGVNRVDDPSKE
                     KGYKLVGDVAFDEVKEVASHLTPVPGGVGPMTIAMLLRNTVRAAQHADAKL"
     gene            625..>1265
                     /locus_tag="AC16V7_15515"
     CDS             625..>1265
                     /locus_tag="AC16V7_15515"
                     /inference="COORDINATES: protein motif:HMM:NF019413.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HIR6258579.1"
                     /translation="MMPDSGQTFPKLPLVIIACQVFQDLIRRLITDEMEAQIVFLDYG
                     LHQVPKKLTQALQDTIDRIDQPSLVVLGYGLCGNGLHGIKAGKHFLLVPRADDCIAIL
                     LGSYSAYRKEFDSSPGTYYLSKGWLESGSNPLSEYKEYEARLGSEKAMWVMDKQYQHY
                     KRLVMVAHSPEDLEKYRPMALDVARFCERWGMRYEEILGSDSFVRKLIEVASLP"
BASE COUNT          314 a          273 c          347 g          331 t
ORIGIN      
        1 taccttgcac tcctgatgga gtgatttatt tactcggtca ggttggggtt gaattagaag
       61 gctgcaatgc ggttgtcttg ggacgctcga acatcgttgg aatgcctgtt gcactcttgc
      121 tagttgagcg caatgctaca gtgacaattt gccactcacg cacacgcaac cttgcggatg
      181 tatgccgctc tgcagacgta cttgtggctg ctgtgggacg accagagatg gtgcgcggcg
      241 gttgggtcaa gcccggagca gtggtgatcg acgttggcgt gaaccgagtt gacgacccca
      301 gtaaagagaa gggatacaaa cttgtagggg atgtggcctt cgatgaagtc aaagaagtcg
      361 cttcgcatct cacaccagta cccggcgggg ttggaccgat gaccattgcc atgttgttac
      421 gcaatactgt gcgcgctgca cagcatgcag acgctaagtt gtgagacctc aatatctaaa
      481 ttgtcttagc cttttcggtt tcaaattagc tcggagatag ggtcacaaag ggtgattatt
      541 actcgttggg accctatctt gtgtttttca gcaaaaggaa ggtaaaattt tagagtaaag
      601 tttatcggta acggatgagg agtgatgatg ccggattcag gacagacttt ccccaaatta
      661 ccgctcgtaa tcatcgcctg ccaggttttt caggatctaa ttagacgatt aattactgac
      721 gagatggaag ctcagattgt attcctggat tatggcttac accaggtacc caaaaagctg
      781 actcaagccc tacaggatac tattgaccgc atcgaccagc ctagcctggt ggttcttggt
      841 tatgggcttt gcggaaacgg tttgcatggc attaaagcgg gtaagcattt cctgctcgtg
      901 ccgcgggctg atgattgcat tgccatttta ttaggttcat acagcgcata tcgcaaggaa
      961 ttcgactctt cacctgggac gtattacctc tccaaaggct ggttggaatc tgggagcaat
     1021 cctttgtcag aatacaaaga atacgaagcg cggcttggtt cagaaaaagc catgtgggtg
     1081 atggataagc aatatcagca ttataagagg ctcgtaatgg tcgcccattc accagaggat
     1141 ttagagaaat accgacccat ggcactggat gtggctcgat tttgcgaacg gtgggggatg
     1201 cgttatgaag aaatcctggg atctgatagc ttcgtccgca aactcataga ggtggcgagc
     1261 ttgcc
//
LOCUS       DBNHOH010000861         3194 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1722013, whole genome shotgun sequence.
ACCESSION   DBNHOH010000861 DBNHOH010000000
VERSION     DBNHOH010000861.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1722013"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="AC16V7_15520"
     CDS             complement(<1..1041)
                     /locus_tag="AC16V7_15520"
                     /inference="COORDINATES: protein motif:HMM:NF014094.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HIR6258580.1"
                     /translation="MADFDYGNARLRAMKSRLLAQRELETLAELGSMHGLISALAKTA
                     YQRSIETALTHASGLECIAQAIRMDLINTFGKARGFFANDAENMVAIVLRPYDLHNLK
                     AILRGLSRNIPSAEILNVLLPVGDLHYAVLAELTRVPGPRVAIDLLASMNEPIVQPLL
                     KLRARHPGADLPEMELALDQWHYQEAWIFVQATPSAHEVLASALKIEADTNNLLTVLR
                     FAHEPEERRHLLESGGAGGVIELLVEPGFLPRPLLVNLGNQDNLEAAIDLLAGTRYKP
                     ALRMGLQAFKKTGRLSEIEKHLKRYRLNWLKNLIFKDALGIGVFLGYVALKINEINNL
                     RWIAHGIHFGLEP"
     gene            complement(999..1640)
                     /locus_tag="AC16V7_15525"
     CDS             complement(999..1640)
                     /locus_tag="AC16V7_15525"
                     /inference="COORDINATES: protein motif:HMM:NF014093.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HIR6258581.1"
                     /translation="MSLESILAAIRAGGDAQFREIESGARTQIYEIMAIARMEAEDIE
                     EKACNDAVAPSAKERARIIHRARLESMHIVGETCEGLINTTLERTRGVLAGLRTDRAY
                     PDVLCRLLEEALQELRSSLGDEGLIHLEADPRDADLLQSMLRQMGLELDVSFTLDSWG
                     GLIAKSEDGRVVVANILETRLERALPYLRQYLAAYFEDEQWQTSTTVTHAYAP"
     gene            complement(1642..1857)
                     /locus_tag="AC16V7_15530"
     CDS             complement(1642..1857)
                     /locus_tag="AC16V7_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HIR6258582.1"
                     /translation="MEGLTAIGAGLAIGLAAIGTGLAQARIGSAGAGVIAEKPEALGP
                     IILLIAIPETMVILGFAVAAMVLLLGG"
     gene            complement(1903..>3194)
                     /locus_tag="AC16V7_15535"
     CDS             complement(1903..>3194)
                     /locus_tag="AC16V7_15535"
                     /inference="COORDINATES: protein motif:HMM:NF013647.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HIR6258583.1"
                     /translation="LLGREDVSRLRLPAELGEGSPDVVMAALRRRLLAIPEELKSIEQ
                     ELAGLAVQWRNRLLSWREALREELDSTSILSRFGETDMTFVLVGWVPVVDLEKTREAL
                     RNIVDERIFIQRLPLTPELKKRAPVSLSNLLPAKPFESLVNLLALPRYGHIDPTLLMA
                     IFLPIFFGMILGDVGYGVLLLILCLGLIRRFKAGVARDVLTILAIGAGWSVLFGFLYG
                     EAFGTLGEHWGMHALWFDRASPENVAGLLVMTIAIGAAHITLGLVLGVWEAYRDRSRS
                     HLLERSGMLVGLIGLFFLVGVLVDFLPQALMTPAIVGIIIGIVLVGTPLGWLGILMGP
                     IEFISLIGNVLSYLRIAAIGLASVYLAKVANDMAGMVGNLIVGVILAILIHALNLVMG
                     AFSPTIHSLRLHYVEFFRKFYEGGGRPYQPFRSQVAK"
BASE COUNT          824 a          937 c          764 g          669 t
ORIGIN      
        1 gggttccaac ccaaagtgaa tcccatgcgc gatccagcgc aggttgttaa tttcattaat
       61 cttgagtgcc acataaccca agaagacgcc gatgcctaac gcatctttga agatcaagtt
      121 cttgagccaa ttcaagcggt agcgcttcag gtgcttctct atctcgctca gacgaccagt
      181 ttttttgaaa gcttgcaacc ccatcctcaa tgcaggctta tagcgcgtcc ccgctagcaa
      241 gtcgatggct gcttccaggt tatcctggtt gcccaaattc acgagcagag gcctcggtaa
      301 aaagccaggc tcgaccagaa gctcgatcac tcccccagca cccccgctct ccaataagtg
      361 tctacgttct tctggctcat gggcaaaacg cagcacggtg agcaggttat ttgtatccgc
      421 ttcgattttt agcgctgagg ccagcacctc gtgagcagag ggcgtggctt gaacaaatat
      481 ccaggcttcc tggtaatgcc attgatccag tgccagctcc atctctggaa ggtccgcacc
      541 cggatggcga gcgcgcaact tgagcagggg ctggacaata ggttcgttca tgctggctag
      601 caagtcaatg gctacacgcg gaccaggcac cctagtcagc tcggccaaca cggcgtaatg
      661 caaatcgccc accggcagca atacgttgag gatttctgcg gaaggaatat ttcgggaaag
      721 accacgtaaa atcgccttga gattgtgcag gtcataaggt cgcaagacaa tagctaccat
      781 attttcagca tcgttggcaa agaaaccccg cgctttccca aacgtgttga tcaaatccat
      841 tcgaatcgct tgcgcaatgc attccaggcc tgaggcatgt gtcagggcag tttcaatcga
      901 tctctggtaa gctgtcttcg ccagcgcaga gatcaatccg tgcatgctgc ccaattctgc
      961 tagcgtttcg agctcgcgct gtgctagcag acgggacttc atggcgcgta agcgtgcgtt
     1021 accgtagtcg aagtctgcca ctgctcatcc tcaaagtaag cagccaggta ttgacgcaaa
     1081 tagggaagag cgcgctccaa gcgcgtctct aaaatgttgg cgaccaccac acgtccatct
     1141 tcactcttgg caatcaagcc tccccagcta tccagggtaa aactcacatc cagctccaac
     1201 cccatttggc gcagcatact ttgcaaaaga tcagcgtcgc gaggatcagc ttctaagtgg
     1261 atgagacctt catctcccag agagcttcga agctcctgca aagcctcttc aagcaggcgg
     1321 cagagcacat cgggataagc tcgatcggtg cgcaagccag ccagtacgcc tcgcgtccgc
     1381 tcaagggtcg tattgatcaa cccctcgcag gtttcaccaa ctatgtgcat ggattcgaga
     1441 cgcgcccggt ggatgatgcg ggcgcgttcc tttgcagatg gcgccactgc atcgttgcag
     1501 gctttctcct caatatcctc agcttccatg cgggcaattg ccatgatctc gtagatttga
     1561 gtacgagcac ctgactcgat ctcgcgaaac tgagcatcac caccggcgcg tatagctgcc
     1621 aggatagatt caagactcat ttcacccgcc caagagcaag accatagccg ccacagcaaa
     1681 gcccaaaatt accatcgtct ccgggatggc gatgagcaag ataattggac ccagggcttc
     1741 tggcttctcg gcgatgacac ctgctcccgc cgatcctatg cgcgcttgag ctaacccagt
     1801 gccaatagca gccaggccaa tggccaggcc agcaccaatt gcagttaatc cttccatgtg
     1861 aacctccagt tgaagggatg tatacgacaa cccttcgcta atttacttgg caacttgact
     1921 gcggaatggc tgatatggtc taccgccacc ttcgtagaac tttcggaaaa attcaacata
     1981 atgcaggcgt aaactgtgga tagtgggact aaaggcaccc attaccaggt tcagcgcatg
     2041 gatcaggatt gccaggatta cgcctacaat caagttaccc accatcccag ccatatcatt
     2101 ggctacctta gccagataga ccgaagcaag cccgatagcg gctatgcgca aataagacag
     2161 gacgtttccg atcaggctga tgaactcaat gggtcccatg aggataccca accaccccag
     2221 tggtgtaccc accaatacaa tgccaatgat gatgcctaca attgctggtg tcatcaatgc
     2281 ctgtggcaga aaatctacca agacaccaac caggaagaat aagccgatca gccctaccag
     2341 catcccactc cgctccaata aatggctgcg gctgcggtcg cggtaagctt cccacacacc
     2401 taacactaat cctagggtga tatgcgcagc gccaatagct attgtcatca ctagcaaacc
     2461 ggcgacattc tccgggctgg ctcgatcgaa ccagagtgcg tgcatccccc aatgctctcc
     2521 aagcgtccca aaagcctccc catacaaaaa gccgaacaaa acagaccaac ctgcacctat
     2581 cgctaatatg gtaagcacat cgcgggccac gccggctttg aaacgacgta tcaacccaag
     2641 gcagagaatt agcagcagga caccataacc cacatccccc aagatcatgc caaagaatat
     2701 cggcaaaaat atggccatca atagcgttgg gtcgatatgg ccatagcgcg gcaacgccag
     2761 caggtttacc aggctctcaa acggcttggc tggcaggagg ttgcttaggg aaaccggggc
     2821 gcgctttttt aattcaggag tcagcggaag cctctggatg aaaatccgtt catcaacaat
     2881 attacgcaaa gcttcacgcg ttttttccag gtcaaccacc ggaacccacc ctaccaagac
     2941 gaaggtcata tctgtctcac cgaaacggga gaggatactg gtggaatcca gctcctctcg
     3001 gagtgcctcg cgccaagaga gcaggcgatt tcgccattgc accgccaatc ctgccaattc
     3061 ctgttctatc gatttgagct cttcagggat agccagtaag cgacggcgta acgcagccat
     3121 gacgacatcc ggagagcctt cacctagctc agcaggtaag cgcagccgcg aaacatcctc
     3181 acgccccagc agac
//
LOCUS       DBNHOH010000862         8274 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_247622, whole genome shotgun sequence.
ACCESSION   DBNHOH010000862 DBNHOH010000000
VERSION     DBNHOH010000862.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8274)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8274)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8274
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_247622"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="AC16V7_15540"
     CDS             complement(<1..466)
                     /locus_tag="AC16V7_15540"
                     /inference="COORDINATES: protein motif:HMM:NF026066.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR6258584.1"
                     /translation="MSANKKNYTNADLAGIFQTIADMLEIKGEVIYKILAYRKAAESL
                     TNLGRDVSDVWREGKLTEIPGVGKAIAEKIDELLTTGRLEFFEKLKSEVPVSLVELLQ
                     VPDLGPKKIALFWRELDITDLPGLEAAARGGKLRGLPGMGEKSEAKIIAGIES"
     gene            complement(475..1902)
                     /locus_tag="AC16V7_15545"
     CDS             complement(475..1902)
                     /locus_tag="AC16V7_15545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR6258585.1"
                     /translation="MPHYDWTDDGYFHTEQRDLTQIRRVLIITMLLNFLAMAIKIVTG
                     LLTGALSVLADGLDSLFDGLSNVVGLAGLYAAAKPPDADHPYGHRKFETLAALSISFL
                     LFLTCWQLLQTAWQRLGQDHLPDINLWTAVVMLVSMLIQAITSLYELRKGRKLQSEIL
                     VADALHTRASILVSLSVLIGLGFVRLGYPQADALLAAFVALMIAKIGVDILRETLPVL
                     VDEAAIDPAQIAEVVSSVSGVESFHRVRSRGADGSALVDLHVRVSPNVALQEADAIAK
                     EVRRRLLELDKVTDVTVHVEAQRYSQTDGADLFATLKHTASELGLVIHESWAHRLDGK
                     LVVEVHVGVDPALTLGEAHDLVDRLEVELRRRSPEIEVVHTHIEMADLQVGEGQRAPL
                     ELEQRVRQQVEQSVAEIAGLSRPHNIILRRNRPDSEYYHIALECHIAPDTPVGDAHQL
                     SHLLEGEIKRRLPEVVEVFVHLEPG"
     gene            2086..3219
                     /gene="dnaN"
                     /locus_tag="AC16V7_15550"
     CDS             2086..3219
                     /gene="dnaN"
                     /locus_tag="AC16V7_15550"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR6258586.1"
                     /translation="MKVSVLQENLAHGLSIVSRAVSARSTLPVLSNVLVATDEGRLRL
                     SATNLELGITCWIGAKITEEGSTTVPARTFTELVSTLSDKQVDMDLNVRTQTLNVHCG
                     ASNTDLKCIDSQEFPPMPTPDLAHGIQINVADLKEMIQQVAFAASTDEARPILTGVLV
                     DVEDNQITLSAADGFRLSIRHAELSSPVPGAMRAVIPARALTELARISADGDQVITMV
                     LPENRGQVIFGMENAELASQLIEGSFPDLQQLVPASYNTRAVLSTSQFLKACKQAEIF
                     AREGSHIARINILNGGEQDPGQVEISGQSDETGFNQTVVDATIEGDPQLIAFNVRFLR
                     EVLDVIKTPNVALETTSGNSPGVIRPVGEDNFLHVIMPMHLGS"
     gene            3302..5626
                     /locus_tag="AC16V7_15555"
     CDS             3302..5626
                     /locus_tag="AC16V7_15555"
                     /inference="COORDINATES: protein motif:HMM:NF013491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR6258587.1"
                     /translation="MTLPPPSADRTLNIYLSLAQFPILSGKIRARMRRELFERGVISL
                     QAFEAEVREKAIRSQSMEGVNNPFSEEPADIWELRLVRVRDHLTDFYFAYNLPYELFE
                     RIVRQVLTERGTPEHEVTVAFNPELAPQSMLFEQADAIEKLPADDRARMEARLREIKV
                     VLIRTMISDHLAYVNIAKEWFTIADLKKIRERKIGQGKIGGKSAGMLLAARILNEAGD
                     DDIRASLRYPESYFIGADLTYGFMSLNSLMHWGDQKYKTEEQIRAEFPELQEEFLAGE
                     FPEDILDSLRGLLEQVGGQPIIVRSSSLLEDSFGTSFAGKYESHFCPNQGSPEENLES
                     LTHAIKRIYASIFLPDALLYRRAKGLQDYDERMAILIQMVVGERFGRYFLPQASGVAF
                     SRNLYRWSPQIRREAGFSRLVWGLGTRAVDRVGNDFPRLVALSHPLLHPQDNTKDIRG
                     YSQRYVDLIDLQENMFKTLPVQEVLQARYPALRYLAQLDQGGYLAPLRSMTMESKTER
                     LVLTFDELFRRTPLAERLSTIVQLLETHYRSPVDTEFAAQIVDPNALQPEVIINLLQC
                     RPQSHLKDSEVHLPKHLPDEDIIFSTRRMAPRGHISEIRYVVFVTPEGYFSLPTQAAR
                     AEMSQVIRQLNRELAEEQFILLGPGRWGTSNPDLGIHIAYGDIYHTRALVELAGQGIG
                     QAPEASFGTHFFQDLVESSIYPLAIYLDDENTIFNRAFFYKTPNRLKNYLDEETPWTD
                     CVRVIEVASYRRGHHLELVMDDEEGISTAFLVAD"
     gene            5626..6282
                     /locus_tag="AC16V7_15560"
     CDS             5626..6282
                     /locus_tag="AC16V7_15560"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HIR6258588.1"
                     /translation="MKKFVAVAGNIGVGKSTLVAMLCQRLDWQPFYEPVAENPYLADF
                     YRDMRAWAFHSQIFFLTHRLRTHRQLIDHPTSAIQDRSVYEDAEVFAQNLYRQGLITD
                     RDYGAYRELYQVLCDFLPPPDLVVYLRASVPTLLKRITQRDRDYERQITAVYLGQLNE
                     LYEDWTSRFSLCPVLTVPADDLDFVAHPRHLELIVRKIEEKLTGKEEVVFDADEVNGM
                     "
     gene            6583..7416
                     /locus_tag="AC16V7_15565"
     CDS             6583..7416
                     /locus_tag="AC16V7_15565"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6258589.1"
                     /translation="MGTKYLNTANGQIAYDEVGEGPLVLCVPSMGDLRGEYRFLAPRL
                     ADAGYRVVTMDVRGHGESSVEWSDYTVAAIGADMLALIRHLNAGPALLVGTSMAAGAS
                     VWAAAEAEDLIAGLVLIGPFVRDMGPIWLGKLIITPLIGGPWGVALWGKYYSMLYPSR
                     KPEDFEAYTRALQSNLRQPGRKQALQRMLLASKRASEERLGEVSAPALVMMGSRDPDF
                     KDPGAEAKRVAEWLNGTAHMVEGAGHYPHAEMPEMIEPLIVGFARSAFVEKVNVATSG
                     A"
     gene            7394..7984
                     /locus_tag="AC16V7_15570"
     CDS             7394..7984
                     /locus_tag="AC16V7_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR6258590.1"
                     /translation="MSPRAGLDTETVVEAAAEMVDREGLAALTMAQLAKQLGIRTPSL
                     YNHVDGLPGLHRALALRGLRALNEHLTRAAIGKMGSAAVMALADAFRGFIKDHPGIYA
                     ATVESVYLLRTSDEELHVENNRILDIVLAVLAPYGLTGEDALHAVRGLRSVVHGFATL
                     ELSGGFGMPLETDESFRRLVQMLIDSMGRHRQPDAA"
BASE COUNT         1776 a         2235 c         2409 g         1854 t
ORIGIN      
        1 gggattcgat gccggcgatg atcttagcct ccgacttctc gcccatgcct ggcaggccgc
       61 gcagcttgcc tccccgcgcc gccgcctcca gcccgggcag atcggtgata tccagctcgc
      121 gccagaatag ggcgatcttc ttaggaccca ggtcgggcac ctggagcagc tccaccaggc
      181 ttacaggcac ctcgcttttt agcttctcga agaactccaa ccgcccggtg gtaagcagct
      241 cgtcgatctt ctctgcaatg gccttgccca cgccggggat ctcggtcagc ttgccctcgc
      301 gccacacatc gctcacgtcc cgacccaggt tggtgaggct ctcggcagct ttgcggtagg
      361 ccagaatctt gtagatcacc tcgcctttga tctccagcat gtcggcgatg gtctgaaaga
      421 ttccagccag gtcggcattg gtatagtttt ttttgttggc agacatggtg aaatttaccc
      481 tggctccaaa tgcacaaaca cttccaccac ctctggcagg cggcgcttaa tttcaccttc
      541 cagcaagtgc gacaactggt gggcatctcc tacgggtgtg tcgggagcga tatggcattc
      601 cagggcgatg tggtaatact cgctgtccgg acggttgcgg cgcaggatga tgttgtgcgg
      661 gcggctcagc ccggcgatct cagcgacgct ctgctctacc tgctgccgca cacgctgctc
      721 cagctccagc ggggcgcgct ggccttcacc cacctgcaaa tcggccatct cgatatgagt
      781 gtgcaccact tcgatctcgg gggagcgccg gcgtagctca acctccaggc gatcgaccaa
      841 atcgtgagct tcacccaagg tcagagccgg gtccactcca acatgtacct ccacaaccag
      901 cttgccatcc aggcggtgcg cccaggactc gtggatgacc agccccagct cgctggcggt
      961 gtgcttgagg gtggcgaata ggtcggcgcc gtcggtttgg gaatagcgtt gcgcttcaac
     1021 gtggacagtt acatcggtga ctttatccag ttccagcagg cggcggcgga cttctttggc
     1081 gatggcatcg gcctcctgga gggctacatt gggagagaca cgcacgtgca agtctaccag
     1141 agcgctacca tcggcgccac ggctgcgcac gcggtggaag gattccaccc cgctgacgga
     1201 gctcaccacc tcggcaatct gggccgggtc aatggcggcc tcatccacca gcaccggcag
     1261 ggtctcacgc aggatgtcca cgccgatctt ggcgatcatc agggctacga aggccgctaa
     1321 tagcgcatcg gcttgcgggt agcccaggcg gacgaatccc aagccaatca gcacagacag
     1381 ggagaccagg atgctggcac gcgtgtgcag ggcatcggct accagtatct cgctctgaag
     1441 ctttcgtccc ttgcgcagct cgtacaggct ggtgatagcc tggatcagca tggatactag
     1501 catgacaacc gcggtccaca ggttgatgtc tgggaggtga tcctgtccca ggcgttgcca
     1561 ggcggtttgc agcaattgcc agcaggtgag gaagagcagg aaggagatgc ttaatgcggc
     1621 aagcgtctca aatttgcgat gcccgtaagg atggtcagca tcaggaggtt tcgccgcggc
     1681 gtacagccca gccaggccga ccacattgga aaggccgtcg aacaggctgt ctagcccgtc
     1741 ggcgagcaca ctcagcgcgc cggtcaggag gccggtgacg atcttgatcg ccatcgccag
     1801 gaagttgagc agcatggtga tgatcagcac gcggcggatt tgggtcaaat cgcgctgctc
     1861 ggtgtggaaa tatccgtcgt cagtccagtc gtaatgaggc atggtgtgga cgaatcatac
     1921 cattttttca tcgttgccca cagcctctat acggttattc ggcgtccatt ttttcggtgt
     1981 gaaggcaaaa ccaatgcgat tgccttgacc caatttctaa aatttcttca caaaatatct
     2041 taggcgttgt atactcatac taaccttaat tggagagtcg ttcttatgaa agtatctgtt
     2101 cttcaggaaa atttagctca tggactgagt atcgtctcgc gcgccgtctc tgctcgcagc
     2161 accctgccgg tgttgtcgaa tgtgctggtg gcaacggacg agggtcgcct gcgcctgtcg
     2221 gctaccaatc tagagttggg cattacctgt tggatcggtg ccaagatcac cgaagagggc
     2281 tccaccactg tgccggcgcg caccttcacc gagctggtca gcacgctctc ggataaacag
     2341 gttgatatgg acctcaatgt gcgcacgcaa actttgaatg tgcattgtgg cgcctcgaat
     2401 accgatctga agtgcatcga ttcgcaagag tttccgccta tgcccacacc cgacctggcg
     2461 catggcatcc agatcaatgt ggctgacttg aaggagatga tccagcaagt tgcctttgct
     2521 gcctcgaccg acgaagcgcg gccgatcctc accggcgtgc tggtggacgt ggaagacaat
     2581 caaatcacgc tttcggcagc ggatgggttt cgtctttcta tccgccatgc tgagctttca
     2641 tcgccagtgc cgggcgctat gcgcgccgtc atcccagcgc gggcattgac cgaactggcg
     2701 cgcatctcgg cagatggcga tcaggtgata accatggtcc tgccagagaa ccgcgggcag
     2761 gtgatcttcg gcatggagaa tgcggagctg gcctctcagc tgattgaggg ctcattccca
     2821 gaccttcagc agttggtccc agccagttac aacacccgcg ccgtgctctc cacttcccag
     2881 ttccttaagg cttgcaagca agctgagatc ttcgctcgcg aggggtcgca tatcgctcgc
     2941 atcaacattc tcaatggtgg cgaacaggac cccgggcagg tggagatatc gggacagtcg
     3001 gatgagactg gtttcaacca gacagtggtc gatgcaacga ttgagggaga cccgcagttg
     3061 atcgcattta atgtgcgctt cctgcgtgag gtgctggatg tgatcaagac acccaatgta
     3121 gccttagaga ccacatctgg gaacagtccc ggcgtcatcc gtccggtagg agaagataat
     3181 ttcctccacg tgatcatgcc aatgcattta gggagctaac cctggacggt tgcgggttga
     3241 aggttgtagg ctatccacga gagcaaacct tcagccttca accattgatt ctgcaacctg
     3301 tatgaccctg cccccgccgt ctgcagaccg cacgctgaat atttatcttt ctctggctca
     3361 atttcccatc ttgagcggca agattcgcgc ccgcatgcgg cgcgaattgt ttgagcgcgg
     3421 cgtgatcagc ctgcaagcct tcgaggctga agtgcgtgaa aaggcgatcc gctcccagtc
     3481 gatggaaggg gtgaacaatc cattttcgga ggaacccgct gacatctggg aactgcgctt
     3541 ggtgcgggta cgcgaccacc tgacagattt ctactttgca tacaacctac cgtatgagct
     3601 gttcgagcgc atcgtgcgcc aggtgctgac cgaacgcggc accccggagc atgaggtgac
     3661 agtagctttc aacccagaac tggcacccca gagcatgctt ttcgagcagg ctgatgccat
     3721 cgagaagcta cctgcggatg atcgcgctcg catggaagcc cgcctgcgag agatcaaggt
     3781 tgtgcttatc cgcaccatga tcagcgatca cctggcgtat gtcaacatcg ccaaagagtg
     3841 gttcaccatc gccgacctaa agaaaatccg cgagcggaaa attggtcagg ggaagatcgg
     3901 ggggaaatcc gccggtatgc tgctggctgc ccgtatcttg aacgaggccg gagacgatga
     3961 tatccgcgcc agcctgcgtt atcccgagtc gtattttatc ggcgctgacc tgacctatgg
     4021 ctttatgtcc ctcaattcgc taatgcattg gggggaccag aagtataaga ctgaggagca
     4081 gatccgggcc gagtttcccg agctccaaga agagttcctg gccggggagt tccccgagga
     4141 tatcctggac agtttacggg gtttgttgga gcaggttggg ggacagccaa tcattgtgcg
     4201 atcatccagc ctgctggaag atagctttgg aacgtctttc gccggtaagt atgagagcca
     4261 tttctgtccc aaccaggggt caccagagga gaatctagag agtcttacgc acgccatcaa
     4321 gcggatttat gccagcatat tcttgcccga tgccctgctc tatcgccgcg ctaaggggtt
     4381 gcaggattac gacgagcgca tggcgatcct gatccagatg gtggtggggg aacgtttcgg
     4441 gcgttatttc ctgccgcaag cgtctggcgt ggcatttagc cgcaacttgt atcgctggtc
     4501 gccgcagatc cgccgcgagg ctggtttctc gcggttagtg tggggcttgg gtacgcgggc
     4561 tgtggaccgg gtgggcaacg acttcccccg gctggttgcc ctcagccacc cgcttctaca
     4621 cccacaagac aacaccaagg atatccgcgg ctattcccag aggtatgttg acctgattga
     4681 tttgcaggag aacatgttta aaacgctgcc tgtccaggag gtcctgcaag cgcgctaccc
     4741 cgcgttgcgc tatttggctc agttagatca gggcggctat cttgcgccgc tacgtagcat
     4801 gacgatggag tccaagaccg agcggctggt gctgacattt gatgagctgt ttcgccgcac
     4861 accattggca gagcgcctga gcactattgt gcaactcttg gaaactcact atcgctcgcc
     4921 tgttgacact gaattcgctg cccaaattgt cgatccgaat gcgctccaac cagaggtgat
     4981 aatcaacttg ctgcagtgcc gtccacagag ccacctcaaa gatagtgagg tgcacctgcc
     5041 aaagcatctc ccagacgaag acatcatctt ctccacccgg cgcatggctc cgcggggtca
     5101 tatctccgaa atccgttatg tggtcttcgt cacccctgag ggttatttca gcctgcccac
     5161 gcaggcagcg cgggctgaga tgagccaagt catccgccag ctaaatcgag aactggctga
     5221 ggagcaattc atcctgctcg ggccgggacg ctggggtacc tcgaaccctg acctgggcat
     5281 ccacattgcc tacggagaca tttaccatac gcgcgccctg gtggagctgg caggtcaagg
     5341 gattggccag gcaccagagg cttccttcgg cacgcatttc tttcaggatt tggtggagtc
     5401 gagcatctat cccctggcga tttacctgga cgacgagaat acgatcttca accgagcttt
     5461 cttctacaag actcccaacc ggctgaaaaa ttatttggac gaagagaccc cctggacgga
     5521 ttgtgtccgc gtaatcgagg tggcttcgta ccgtcgtgga catcacttgg agctggtgat
     5581 ggacgatgaa gagggcatct ccactgcttt tctggtagct gactgatgaa aaaatttgtc
     5641 gctgtcgctg ggaatattgg tgtgggtaaa tccacgctgg ttgccatgct ttgccagcgg
     5701 ttggactggc aaccattcta cgagccggtg gcggagaacc cctacctggc agatttctac
     5761 cgcgatatgc gcgcctgggc tttccactcg cagattttct tcctcaccca ccggctgcgc
     5821 actcaccgcc agttgatcga ccatcccacc tcggcgatcc aggaccgcag cgtctacgag
     5881 gatgcagagg tctttgctca aaacttgtac cgccaggggc tgatcactga ccgcgattac
     5941 ggcgcttacc gtgagctgta ccaagtgctg tgcgatttcc tgccaccgcc cgacttggtg
     6001 gtgtacctgc gcgcctcggt gcccacgttg ttgaaacgca tcacccagcg tgaccgggat
     6061 tatgagcgcc agattaccgc tgtttattta ggtcagctca atgaattgta tgaggattgg
     6121 acgagccgct tcagcttgtg ccctgtgctg acggtgccgg cggatgacct ggattttgtg
     6181 gcccaccccc gccacttgga gttgattgtg agaaagatcg aggaaaagct gaccgggaag
     6241 gaagaggtgg tctttgatgc ggatgaggtg aatgggatgt agggaagtgt accataccga
     6301 ctgggaagca caggtattta ttttttacgg tattgtagca aatgaggttg taaaaaccac
     6361 gcaaggtgat catgtaggag atagccacgc cgctcatttg gcagcgtggc tatctcatat
     6421 tttgcttctg tgacagcatt gctagcccac gcgatgactg tttttttgag ataacccgat
     6481 cttcaatctg aatctgagat ttacccttga caaactaata atattagtta taatactaac
     6541 actgttagtt tatactggtt gaagataaac ggaggtattc aaatgggaac aaaatactta
     6601 aacaccgcta atgggcagat tgcatatgac gaagtcggcg agggtccatt ggttttgtgc
     6661 gtgccctcta tgggcgatct gcgcggcgaa tatcgcttcc ttgcacccag gctggctgat
     6721 gctggttacc gcgtggtaac tatggatgtg cgcgggcatg gggagagcag tgtcgagtgg
     6781 agcgattaca cggttgcggc aattggtgcc gatatgcttg ccctaatacg gcatctaaat
     6841 gccggacctg cgctgctggt tggcacctca atggcagccg gcgcatcggt atgggccgcg
     6901 gcagaagccg aagatctgat cgcaggtctg gttttgattg ggccttttgt acgcgatatg
     6961 ggaccgattt ggctgggaaa gctaatcatc accccgctga ttggcggccc ttggggagtc
     7021 gcgctttggg gaaaatacta ttccatgctc tatccaagca ggaagcccga ggattttgag
     7081 gcctacaccc gtgctctcca atccaatttg cgccagccag gacgaaagca ggcattgcag
     7141 cgcatgctct tggcctccaa gcgagcctcg gaggaacgac ttggagaggt gtctgcgcct
     7201 gcgctggtta tgatgggctc acgtgaccca gatttcaaag atccgggagc ggaagcaaag
     7261 agagttgctg aatggctgaa tgggacggca cacatggtgg aaggtgcagg tcactatccc
     7321 catgctgaga tgccggagat gattgagccg cttatcgttg gcttcgccag gtcggccttt
     7381 gtggagaagg taaatgtcgc cacgagcggg gcttgatacg gagacagttg tcgaagcggc
     7441 ggcagagatg gtggaccgcg aagggctagc cgcgctgaca atggcccagc tagcgaagca
     7501 gctcgggatt cggacgccat ctctctacaa ccatgttgat gggcttccag gactccaccg
     7561 ggcgctggcg ctgcgtggct tgcgagcgct gaatgagcat ttgacccgcg ctgcaattgg
     7621 gaaaatgggt tctgcagctg tgatggcgct ggcggacgct ttccgggggt tcatcaagga
     7681 tcaccctggt atttatgcag caacggtgga atcggtatat ttgttaagaa cctctgacga
     7741 ggaactgcat gttgagaaca accggatcct ggatatcgtc ttagctgttc ttgctcccta
     7801 tgggcttacc ggcgaagatg ccttgcacgc tgtccgtggg ctgcgcagcg tggttcacgg
     7861 ctttgcgact ttagagctct ccggtggttt tggaatgcca ttggaaaccg atgagagctt
     7921 tcgccgcctg gtccagatgc tgattgacag catggggcgg cacagacagc ctgatgcagc
     7981 gtgatagtga gccagcacga tgaggcgaag accaggcctc agttttggcg gaagatggaa
     8041 tatagccaag gaataacacg aatcaacccg gattgatgcc gggttggggt aatgactaga
     8101 gatagattac cacagattta tgcagtagag ggacattgaa cacagataca cactgataca
     8161 tacagatgca cacggatcaa cgcaaaataa tagagaggag gaataatgac tccaggagat
     8221 caagcatcaa cagagagagg gagtagacag aatttatatg ctgatttatc gagc
//
LOCUS       DBNHOH010000863         3103 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1081871, whole genome shotgun sequence.
ACCESSION   DBNHOH010000863 DBNHOH010000000
VERSION     DBNHOH010000863.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1081871"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="AC16V7_15575"
     CDS             complement(<1..406)
                     /locus_tag="AC16V7_15575"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR6258591.1"
                     /translation="MAKKTALVLSGGGAKGAFQCGAEKYAREVKGYKWDIIAGVSVGA
                     LNGCMLAMEKYERLFEVWNTISNDQVYTGGFNIWSGIKLLFGAKSFYSNDPLRRMLSQ
                     ELEPDKIKTDLRVGAVSLVSGEYIEFRADSPNL"
     gene            823..1305
                     /gene="nrfH"
                     /locus_tag="AC16V7_15580"
     CDS             823..1305
                     /gene="nrfH"
                     /locus_tag="AC16V7_15580"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF015237.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HIR6258592.1"
                     /translation="MKLALIVGFTALVIVIGVGLYVTDFTAYLGNNPTTCNNCHVMDA
                     VYESWYHGGHKQWANCSECHTPHALIPKYIVKAENGYHHVSAFILGRIPDAIRSREVS
                     RQIVQENCVRCHAETASTILEGPMAFDRYCFDCHRSVAHGERGISILPYQHSEEYIER
                     "
     gene            1302..2606
                     /locus_tag="AC16V7_15585"
     CDS             1302..2606
                     /locus_tag="AC16V7_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HIR6258593.1"
                     /translation="MKRTSIVFILSVIIVILAVALVGTLLFIKNQPASTRGVAPLTQI
                     DEYEPDSSKWGVNFPNQYSTLKLTEINKARSTYGGSEPFSKLEDDPRLVTLFAGYGFS
                     KDYNEERGHLNSLTDVRATLRVNETTPGTCYSCKSSDNPRLWAEMGMAEFDKTLFKDL
                     GAQISNPIGCANCHEPNTMRLIVTNPALEEALQAQGKDWRTFTRQEMRTVVCANCHVE
                     YYFAGEGKYLVFPWKDGSKIENIAQFYADLEFKDWEHPQSGAPMIKMQHPEYEFFTAD
                     STHYKAGVACADCHMPYTRDGAVKFSTHDVKSPLQNADTACGACHTDVTYVTGRVAII
                     QDQVRETMDASEDALVAAIQAIEAATAATDVNADLLGEARRLHREAQLRWDFIAAENS
                     MGFHNPEEALRILANATDLARQAQLVAVQAAGTPSIIQTSSQ"
     gene            complement(2608..>3103)
                     /locus_tag="AC16V7_15590"
     CDS             complement(2608..>3103)
                     /locus_tag="AC16V7_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258594.1"
                     /translation="AELIRDWLAQCGIAVNLQYLEREALFAPGPDGPLFGRRFDMAQF
                     AWPASLQPACFLYSTGEIPGPYPQFPKGWGGANDTGFSNLAFDRACQRARFSLPDSPD
                     YAMAHREAQAVFAEELPAIPLYVHLTWAAMRVDLCGVELQEPVENTLWNLETWDYGPD
                     AVCD"
BASE COUNT          752 a          916 c          790 g          645 t
ORIGIN      
        1 ccaggttggg tgagtcagcc cgaaactcga tatactcgcc gctcaccagg gaaactgccc
       61 caacgcgcag gtctgtctta attttatcag gctccaattc ctgtgaaagc atgcgccgga
      121 gcggatcatt gctatagaat gatttggcac caaagagcaa cttgatgccg gaccaaatat
      181 tgaacccgcc agtatagacc tggtcattgg agatcgtatt ccagacctca aatagccgct
      241 cgtatttctc catagccagc atgcagccat tcagcgctcc aacagaaacc ccagcgatga
      301 tgtcccattt atagcctttc acctcacggg catatttctc cgcgccgcac tggaaggcgc
      361 cttttgcgcc tccaccactg agcaccagtg ccgtcttttt agccatcgtc gcctccttcc
      421 atccatcatg gacttatacc ccttggagta gcaataatca taccatccaa aagtaaaatt
      481 tggactcggg aaaacaggct ggccagggca attgcatatg ccctgaaggg catgcgcaat
      541 cccgcaaaca gagtgtttgg gatgttttgg tgggggcata cccccaccaa aacatcccat
      601 tttctctcct cagtggtggg aaatcgccca aatgcgattg ccctaacagg ttggctgacc
      661 cacgaacagg ctgatattcg tctgaccagc gccgcaacca gtctcactgg tagggtatac
      721 tacaatctat tattgtgaca aatatcacag acctgctgtt tgggtcgcca ggccgttact
      781 ccaaatgcgc gaacaatgaa tcctgacacc aaaggttggg ccatgaaact tgctctcatc
      841 gttggattca ccgcccttgt gatcgtgatc ggagtcgggc tgtatgtcac cgatttcacc
      901 gcatacctgg gcaataatcc aacaacctgc aacaactgcc acgtcatgga tgccgtctac
      961 gaaagctggt accacggcgg gcataagcaa tgggctaatt gttctgagtg ccacaccccg
     1021 cacgccttga tcccaaagta cattgtcaaa gccgagaacg gctatcatca cgtctccgcc
     1081 tttattcttg ggcgcatacc tgatgcaatc cgctctcgag aagtctcgcg ccagatcgtc
     1141 caggagaact gcgtgcgctg ccacgccgag acggcatcca ccatactcga gggcccaatg
     1201 gcttttgacc gctactgctt cgattgccat cgcagcgtgg cacatggcga gcgcggcata
     1261 tcgatcctgc cttaccaaca ttctgaggag tacatagaac gatgaaacgc acaagcattg
     1321 ttttcatttt atctgtcatc atcgtcatcc tggcagttgc cctggtaggc acactgcttt
     1381 ttatcaaaaa ccaacctgcc tcaactcgcg gtgttgcccc cctgacgcag atcgacgagt
     1441 atgagcccga ctcatccaag tggggcgtta atttccccaa ccagtattca accctcaagc
     1501 tgaccgagat caacaaagct cgcagcacct acgggggttc ggagcctttt tccaagctgg
     1561 aggatgatcc tcgtctggtg acgcttttcg ctggatacgg cttcagcaaa gactacaatg
     1621 aagagcgcgg ccacctgaac agcctgaccg atgtgcgcgc caccctgcgc gtcaacgaga
     1681 cgaccccagg cacctgctac tcttgcaaga gctccgacaa cccccgcctg tgggcagaga
     1741 tgggtatggc tgaatttgac aagacgctct tcaaggattt gggggcgcaa atcagcaacc
     1801 cgatcggatg cgccaactgc catgagccaa ataccatgcg cctgatcgta accaatcccg
     1861 ctttggaaga agctctccaa gcacagggca aggattggcg tacgttcacc cgccaggaga
     1921 tgcgcaccgt agtttgcgcc aactgccatg tggaatacta cttcgctggc gagggaaaat
     1981 acctggtctt cccctggaag gacggctcca agattgaaaa catagctcaa ttctatgcag
     2041 acctggaatt caaagattgg gaacacccgc agtccggtgc accgatgatc aagatgcagc
     2101 accccgaata cgagttcttc accgccgaca gcacccacta taaagccgga gtagcctgtg
     2161 ccgactgcca catgccctac acccgcgatg gcgcggtcaa gttctcgaca cacgatgtaa
     2221 aaagcccgct gcaaaatgcc gataccgctt gtggcgcttg ccacaccgat gtgacttatg
     2281 tgaccgggcg ggtggctatc atccaggatc aggtgcgtga gaccatggat gccagtgaag
     2341 atgccctggt ggctgccatt caggcgatcg aggcagccac agcggctacg gatgtaaacg
     2401 ctgatctatt gggagaggcg cgtcgcctgc accgcgaggc tcaattgcgc tgggacttta
     2461 tcgctgcgga gaacagtatg ggcttccaca accccgaaga ggccttacgt atcctggcaa
     2521 acgccacaga tctcgcccgc caagctcaac tagtagctgt tcaagctgcc ggcacgccga
     2581 gtattatcca gaccagtagt cagtaactca gtcacaaact gcatcgggac cataatccca
     2641 cgtttccagg ttccacaagg tgttttcaac cggttcctgg agttcaacgc cgcacagatc
     2701 cacccgcatc gccgcccagg tcaggtgcac atataatggg atagcaggca actcttcagc
     2761 aaagacagct tgtgcctccc ggtgggccat ggcgtagtca ggagaatctg gcaaagaaaa
     2821 gcgggcgcgc tggcaagcgc ggtcgaaagc caggttgctg aaaccagtgt cattcgcgcc
     2881 gccccagcct ttcgggaatt gagggtaggg gccggggatc tcgccagttg agtagaggaa
     2941 gcaggccggt tgcagggagg cgggccaagc aaactgagcc atatcgaagc gccgcccaaa
     3001 cagtgggcca tccgggccgg gcgcgaataa ggcttctcgc tcaaggtatt gcagattcac
     3061 tgcgatgccg cactgcgcca gccaatcccg gatcaattca gct
//
LOCUS       DBNHOH010000864         2872 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_761329, whole genome shotgun sequence.
ACCESSION   DBNHOH010000864 DBNHOH010000000
VERSION     DBNHOH010000864.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_761329"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="AC16V7_15595"
     CDS             complement(<1..559)
                     /locus_tag="AC16V7_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR6258595.1"
                     /translation="MRYRTLGKSGLIVSELALGTMIFGEKSQRSASPSEAVRIIHRFL
                     EVGGNHIDTSNVYAKGRSEEILGDALRGKRQQVVLATKVRFATGSGPNEAGLSRYHVL
                     RSVEDSLRRLQTDVIDLLYMHSWDPLTPLEESLKVFDTLVTSGKIRYIGVSNFKAWQL
                     MKALGLCDAHGWERFIAAQYQYSLVE"
     gene            complement(567..1055)
                     /locus_tag="AC16V7_15600"
     CDS             complement(567..1055)
                     /locus_tag="AC16V7_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258596.1"
                     /translation="MPEQKIFSFDWALCFQWLMATTVGWVLGRFLLPNLAFITIGIGL
                     GVLQWLILQYRIKHAQQWILATIFSWTLGSLVILFLMPDVSDFIAGLVIGVATGTGQW
                     LVLRRELHWAGWWIPINVVAWTTGMALLPGVILTGIMAGLVTGIAIELLLRTPRNLVE
                     VK"
     gene            complement(1187..2290)
                     /locus_tag="AC16V7_15605"
     CDS             complement(1187..2290)
                     /locus_tag="AC16V7_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014370404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR6258597.1"
                     /translation="MGKPTTQRRLGSLFVNGTLFLLVLIWTVPTLGIFVSSFRNRNDI
                     STSGWWNVFPHREWQPVAEIDTKATGLDPDGIMEIEGATGTFEEFRVGIETPDGKRIN
                     WIGNKRLGRVEIQERVWAVNWNFSLDNYNQVWGGQDFEFTKPDGTVEVVPGDDMSRAF
                     FNSLAVSIPSTVIPILIAAFAAYAFAWMRFPGRRWMFILVVALLVVPLQIALVPILRD
                     YKELELNGTFLAIWLAHAGFGLALATYLLYNYISALPREMLESAFIDGASHFTVFLRL
                     ILPLSVPALASFAIFQFLWVWNDYLVALVFLGGNPEFELVTQRMAAIVGARGQDWHLL
                     TAGAFVSMALPLLVFFSLQRFFVRGLLAGSVKG"
     gene            complement(2292..>2872)
                     /locus_tag="AC16V7_15610"
     CDS             complement(2292..>2872)
                     /locus_tag="AC16V7_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR6258598.1"
                     /translation="LIFLPMAISFVGAGIIWNFIYEYRPANQPQVGLLNSIVQGFGGQ
                     PRAWQQWVDIIPWNNLFLIVIVIWLQAGFAMVLFSAALKGIPEELIEAGRIDGANEFQ
                     IFFRIMIPYIQGTLITVWTTIVIFTLKIFDVVWVMTGGQFGTHVIATQFYRQSFTADN
                     KGFGAAIAIILLIAVIPVMIYNLSQFRQQEAF"
BASE COUNT          816 a          776 c          659 g          621 t
ORIGIN      
        1 gctccaccaa gctgtactgg tattgggctg caataaagcg ctcccaacca tgtgcgtcgc
       61 ataatcccaa tgctttcata agttgccagg ctttaaaatt cgacacaccg atgtagcgta
      121 tcttgcccga agttaccagg gtatcgaaca ccttcaagga ttcttccaag ggcgtgaggg
      181 gatcccaaga atgcatgtag agcaagtcga taacgtctgt ttgtaatcgc ctcaagctat
      241 cttcgacgct gcgcaagaca tggtatctag aaagaccagc ctcatttgga ccacttcctg
      301 tagcaaaccg aactttggtg gccaggacga cttgttgtcg tttgccacgc aacgcatcac
      361 cgagtatctc ctctgagcgc cccttggcgt acacattaga cgtatcgatg tgattacccc
      421 caacctccag gaagcgatgg atgatgcgca ctgcttcaga gggagaagca cttcgctggc
      481 ttttttcccc aaatatcatc gtgcctaatg ccagctctga cacgatcaac ccgcttttac
      541 ctaaagttct gtaacgcata gggatgttat ttcacctcta ccaaatttcg cggcgttcgg
      601 agcaacaatt cgatggctat accggttacc aaacctgcca tgataccggt taatatcacc
      661 ccaggtagca aagccattcc tgtagtccag gccacgacat tgatcgggat ccaccatcca
      721 gcccaatgca gctcgcggcg cagcactaac cactgccctg ttccggttgc cacaccgatc
      781 accaagccag cgatgaaatc gctaacatcg ggcattaaaa aaaggatgac caacgaacct
      841 aacgtccagc taaatattgt ggcaagtatc cactgctggg cgtgcttaat acgatattgt
      901 aagattagcc attggaggac accaagaccg attccaatgg tgataaacgc tagatttgga
      961 aggagaaacc tgcctaatac ccagccaacc gtagtcgcca tgagccattg gaagcaaagc
     1021 gcccagtcga atgagaaaat tttctgttca ggcatcaatt gttcgtataa acgacgcgac
     1081 aaaatttaac taatcataac cttgcttgaa caagaagttc aagcatgttg tgattacttg
     1141 gcagttggat tgacttaggt catcttagga aatccgtaaa ggatgcctat cctttcaccg
     1201 aaccggctaa caggccacgc acaaagaagc gctgcaatga gaagaagacc agcaacggca
     1261 gcgccatcga aacaaaggct ccggccgtca acaaatgcca atcctgccct cgggcaccaa
     1321 cgatagccgc catacgttgt gtcacaagct cgaattccgg gttgccaccc aggaaaacca
     1381 gcgccaccag gtaatcgttc catacccaca ggaactggaa tatggcgaac gaggccagcg
     1441 ctgggaccga caatggcagg atcaaccgta agaataccgt aaaatgcgag gcgccatcaa
     1501 tgaacgcaga ctccagcatc tctcgcggca gcgcactgat gtagttgtac aacagatacg
     1561 tagccagcgc cagcccaaaa ccggcatgcg ccagccagat tgccagaaac gtgccattaa
     1621 gctctaactc tttgtaatct cgtaagatgg gaaccagggc aatttggaga gggacaacca
     1681 gcaaggctac aaccagaata aacatccaac gccgccccgg aaaacgcatc caggcaaaag
     1741 cataggcggc aaaagcagca atcagaatag ggatcaccgt agaaggaatg gatacggcta
     1801 ggctgttgaa gaaagccctg gacatatcat cacccggcac cacctctaca gtgccatccg
     1861 gtttggtgaa ctcaaaatcc tggccacccc aaacctgatt gtagttatcc aatgagaaat
     1921 tccaattaac ggcccaaacc cgctcttgaa tttcaactcg tccaagtcgc ttgttgccaa
     1981 tccagttgat gcgttttcca tccggcgttt cgatgcccac ccgaaactcc tcgaatgtgc
     2041 ccgtagcgcc ctcgatttcc ataatcccat cggggtcaag tccggtggct ttggtgtcaa
     2101 tctcggcaac cggctgccac tcccggtgcg gaaacacatt ccaccatcca gaggtagaga
     2161 tatcattccg gttacggaaa gaagagacaa aaatgcccag ggtgggaaca gtccatatga
     2221 ggacaagcag gaataatgtc ccgttgacga acaaactacc gagtcgccgt tgtgtggttg
     2281 gtttgcccat tttagaacgc ctcctgttgc ctgaactgac tcaggttata aatcatcacc
     2341 gggataacgg cgatgagcag gataatggca atggctgcgc caaagccctt gttatcagcc
     2401 gtaaatgatt gccggtaaaa ctgggtcgca atcacgtgcg tgccaaactg cccgccagtc
     2461 atcacccaca ccacgtcgaa tatcttcaag gtgaagataa ctatggttgt ccagacggtt
     2521 ataagtgtcc cctggatgta agggatcata atcctgaaaa atatctggaa ctcgttagcc
     2581 ccatctatgc ggcctgcttc tattaactcc tccgggatgc ctttcagtgc cgcagaaaac
     2641 aataccatgg cgaaaccggc ttgcagccaa atgacgatga cgatgagaaa gagattgttc
     2701 cacggaatga tatcaaccca ttgttgccaa gctctcggtt gacccccaaa accttggaca
     2761 atagaattta gcaggccaac ttgcggttga tttgctggtc gatactcata gataaagttc
     2821 caaatgatgc cggctcctac aaaggaaatg gccattggca aaaatattaa cg
//
LOCUS       DBNHOH010000865         1656 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1466801, whole genome shotgun sequence.
ACCESSION   DBNHOH010000865 DBNHOH010000000
VERSION     DBNHOH010000865.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1466801"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1144
                     /locus_tag="AC16V7_15615"
     CDS             155..1144
                     /locus_tag="AC16V7_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015500608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6258599.1"
                     /translation="MDQIAKFFRSTVAGPLVAFVVVAILASLATPRFLLPQNLSNVSL
                     QVATVAVVSIGATLVILTGGIDLSPGSTVALVTCTLAILVKNMGLPLPLGIFLVLLLG
                     AVCGFINGFFSTFMKIPSFIVTLATMSIYRGLAFLITNGTPIFSVSPELEPIFYGKFL
                     GIPLPFYYVITLYLLGAFFLRSTVQGRAIYAVGGNESAARLTGIRVNRIRLLAFILAG
                     LSASIGGVLLTAWLNSGSPNYGAELGLQSIAAAVIGGASLTGGYGSMIATLIGALTVA
                     VVQNGLNLLAVPASWQEITLGMVIVLAVGLDMWRADIGRGFRRLFRISSGKPG"
     gene            1256..>1656
                     /locus_tag="AC16V7_15620"
     CDS             1256..>1656
                     /locus_tag="AC16V7_15620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4432 domain-containing protein"
                     /protein_id="HIR6258600.1"
                     /translation="MYTYGRKTSCRISLDYTYRGMRVAFLENDLIRVGVLLDKGADIF
                     EFTFKPVDLDFMWQSPIEMRKPFVATSALPEGAFHDYFYGGWQEVLPSAGWASEPYQG
                     TFQGLHGEVSLLPFEANITEDTPEVVSLRTWV"
BASE COUNT          339 a          449 c          453 g          415 t
ORIGIN      
        1 agcggggaag tggtcgctgc gatcagcatc tcaggaccta ccgagaagat gccctccgaa
       61 ccaggcagca gcccaataag ggaggaggtt caatcgattg cagaagccat atccagggaa
      121 tttgccacca atcattcagc aggagagaac ccacatggat caaatcgcta aattcttccg
      181 ctccacggtt gccggccctc tggtggcctt tgttgtggtg gcgatcctgg ccagcctggc
      241 cacaccccgc ttcttattgc cacaaaacct gagcaatgta tccttgcagg tagcaacggt
      301 tgcggttgtc tctattggcg caacgctggt gatcctcaca ggcgggatcg atttatcccc
      361 cgggtccacg gtcgccctgg tgacctgtac acttgcgatc ctggtcaaga atatgggttt
      421 gcccctgccg ttaggtattt tcctcgttct attactggga gctgtctgtg ggtttattaa
      481 cgggtttttc agcacgttca tgaagatccc ttctttcatc gttacccttg caaccatgag
      541 catttaccgg ggattggctt tcttgatcac caatggcacc ccgattttct ctgtctcgcc
      601 tgaactcgag cccatttttt atggtaaatt tttgggtatt cccctgccat tttattacgt
      661 cataacccta tacttgctag gagcgttctt cctgcgcagc acggttcaag ggcgagcgat
      721 ttatgccgtg ggcggcaacg aatcggcagc gcgcctgaca ggcatccgcg ttaaccgcat
      781 ccggctgttg gcctttatcc tggcagggct gagcgcctcc ataggtgggg ttttattgac
      841 cgcctggctg aactcgggtt cccccaacta cggggcagag cttggcttac agtctatcgc
      901 cgcggcagtg ataggcgggg ccagcctgac cggcggctat ggcagcatga tcgccaccct
      961 aattggggcg ttaactgtgg cagtggtgca aaacgggctc aatctgctag ctgtgccagc
     1021 ttcttggcaa gagatcaccc tgggaatggt catcgtgctg gcagtagggc tggacatgtg
     1081 gcgggcggac atcgggcgtg gcttccggcg cctgtttagg atctccagcg gaaaacctgg
     1141 ttaggactgg atcgattaga cacgaaggat cacaaaatac atcaaacatt ttcttccttg
     1201 gtgccacttt gtgacctttg tggcgaagaa ttacctgaga taggagagca tcaagatgta
     1261 cacatatgga agaaagacca gctgccgtat tagccttgat tacacttacc gagggatgcg
     1321 cgtggccttc ctggaaaacg acctcatcag ggtgggtgta ctgctcgaca aaggggcaga
     1381 catcttcgag ttcaccttca agcccgtaga tctcgacttt atgtggcaat cgcccatcga
     1441 gatgcgcaag cctttcgtcg ctaccagcgc ccttcccgaa ggcgcttttc acgattattt
     1501 ctatggtggc tggcaggagg tgctgccctc tgctggctgg gcttctgagc cctatcaggg
     1561 taccttccaa ggcttgcacg gtgaggtttc actgctaccc tttgaagcca acatcacgga
     1621 ggacacgcct gaagtggtct cactacgcac ctgggt
//
LOCUS       DBNHOH010000866         2907 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_697120, whole genome shotgun sequence.
ACCESSION   DBNHOH010000866 DBNHOH010000000
VERSION     DBNHOH010000866.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_697120"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="AC16V7_15625"
     CDS             complement(<1..570)
                     /locus_tag="AC16V7_15625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR6258601.1"
                     /translation="MSALKKISLVLLALVVISGLVAATVQPAAAADNAKTCKQWYTVQ
                     RGDYLVKIARMYDTDWRTLADLNDLKTPERIYPGQKLCVSTDGSGTSAPVPSTSSSSG
                     VRVYASSVEEDKTVTLEGKKLDVNSRYTLYLSKYGTYPTGAILVGSIVTDRYGAFSKT
                     FNLPSKLVDISKIGIHITNGRDATSNWFIN"
     gene            complement(1141..1749)
                     /locus_tag="AC16V7_15630"
     CDS             complement(1141..1749)
                     /locus_tag="AC16V7_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258602.1"
                     /translation="MRYLHLLKTEAVQRGVMPPEVELDPGLVFTLVRDMPYRRASDRK
                     PETTISEWRGTCSGKHYLLQALFAELGFKSQVVACTTVTPIDASEVPETLRLLYEAAN
                     RRFVDVHNYLLLELPAGGQMIVDATWPLSARKAGLPVNETFVLGQDQAIAAEPLESWP
                     VPDGRDPQEFKDKLLREHFSPTELEFREEVIRALGDMTGGDE"
     gene            complement(1803..2177)
                     /locus_tag="AC16V7_15635"
     CDS             complement(1803..2177)
                     /locus_tag="AC16V7_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258603.1"
                     /translation="MKLGTFLTIKAAISFIFGTSSLLAPAQLLSMYGVALDPFGVNIS
                     RLLGVTLLGIGFICWFTRNAAYSELRQGILLSLFIADTLGFIVDLLSQLSGVESGLGW
                     MNVAIWFFLALGLGYFRFIKTG"
     gene            complement(2379..>2907)
                     /locus_tag="AC16V7_15640"
     CDS             complement(2379..>2907)
                     /locus_tag="AC16V7_15640"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HIR6258604.1"
                     /translation="QRVISHPQAVAQCDATLRSLGHVKAEAAYDTAGSVKIVSQQNDR
                     TLAAIASRQAAELYGMQILAENIEDNPANITRFQIIACQPVNPGADAKTSIVFSLKNE
                     PGSLFKALSVFALRDIDLTKIESRPLAGKPWEYFFYIDFAGSTQDEVVSRALSHLEEY
                     ATFLRVLGSYPRHRV"
BASE COUNT          720 a          767 c          762 g          658 t
ORIGIN      
        1 atttataaac cagttggagg ttgcatcacg cccgttggtg atatggatgc caatcttaga
       61 aatatcgacc agcttgctgg gcaggttgaa ggtcttcgag aaagccccat acctgtcggt
      121 gacaatggat ccaaccagga tagcgcccgt ggggtacgtc ccgtatttgc tcagatacaa
      181 cgtgtagcgg gaattgacgt caagcttctt accctccaag gtaactgtct tgtcttcctc
      241 gacagacgaa gcatacactc tcaccccgct gcttgagcta gtgcttggca caggggcgct
      301 agtcccagag ccatccgtgg agacgcacag cttctgcccg gggtagatcc gttcgggggt
      361 ttttaaatcg ttcaaatcag ccagggtgcg ccaatcggtg tcgtacatgc gggcgatttt
      421 taccaggtaa tcaccccgct gtacggtata ccactgcttg caggttttgg cgttatcggc
      481 ggcagcagca ggttggaccg tggctgctac caggccggaa atcacgacca gggctaaaag
      541 aaccagggag atttttttca gagcagacat ttcacacctc ttgcatttga tgattgggct
      601 gtacagcgag ccagcatggg aatttggtct tctctataca atccaaatcg tagacgttga
      661 tatagccaaa tgagttcccg atttgtgcct tttttcccaa ttttgattaa atttggcttc
      721 acttctcttc acagccaccc cataaccatc tatcatgttt ttataggtta gattatagca
      781 agcattgtat aacctgtcaa gttatttttg atggttattt tactgattcg cgagtcaccc
      841 acagcaaaag cttttctctc tcgcggaaag cgtcatagca aaggagtcct tctgtcaact
      901 gaagcacctg cgcgcgaaac ggacgaaact gattcgcctg ccaaaacacg gcaggctcac
      961 agtggcgatt tcataaggaa aattagacac gcaggggaga ataaaccggg cgtgaggcta
     1021 acaggctagg cgatccagcc gttaacgaat cggactaagc cgttgctaaa ttgtcaatgg
     1081 tgtccaagcc tgtaccaccc aacgtcagac tggcatgact ccaagattca agacaaaaat
     1141 ttattcatca cccccggtca tatcccccaa cgccctgatc acctcctcac ggaattctaa
     1201 ttcggtgggg ctgaagtgct ccctcagcaa cttgtccttg aactcctgcg gatcgcggcc
     1261 gtcgggaaca ggccaggact ccagcggctc ggcagcgatg gcttgatcct gacccagcac
     1321 gaaagtctcg ttaactggca agccagcctt gcgcgctgaa agcggccagg tggcatccac
     1381 gatcatctgt ccgcccgcag gcagttccag cagcaggtag ttgtgcacat ccacgaagcg
     1441 acgattggcg gcttcgtaca gaaggcgcaa ggtctcaggt acttcgctcg catcaatcgg
     1501 cgtaaccgtg gtgcaggcca cgacctgcga cttgaagccc agctcggcaa acagcgcctg
     1561 gagcaggtaa tgcttgcccg agcaggtgcc gcgccactcg ctgatggttg tttcgggttt
     1621 gcggtcgctg gcccggcggt agggcatatc ccgcaccaag gtaaagacca gcccaggatc
     1681 cagctcaacc tctggaggca tcaccccccg ctgcaccgct tcggttttca agagatgaag
     1741 atagcgcatc ggggtattgt atcatctgat gcttaatggt tgaaagacgc acgaagacaa
     1801 ggctatccgg tcttgatgaa gcggaagtaa cccaggccta acgccaggaa aaaccagatg
     1861 gcgacgttca tccaacccag cccgctttcc acgcctgaaa gctgggagag caagtcaacg
     1921 atgaatccaa gggtgtccgc gatgaacagg gatagcaaaa taccctggcg cagttctgaa
     1981 taagccgcat tgcgggtaaa ccagcagata aagccaatcc ccagcagggt aacccccagc
     2041 aagcgagaaa tattcacgcc aaatggatcg agggcgacgc catacatgct caataactgg
     2101 gcgggcgcca aaaggcttga ggttccaaag ataaacgaga tggccgcttt gatcgtcaaa
     2161 aaagtgccga gtttcatttg agacctcctt gtagttaaaa aaactgccct ctagcttagt
     2221 tctagagggc ggtttgcgat ggatatggcg gcgatacttt tgaagcaaca atgagctaaa
     2281 caaactactc tagtggagtc cttttcatct cccgtgttgc aacttgatca taccacaagc
     2341 acctgtgtct cgaagggtat ggtttttaat aaccctgctc atacacggtg ccgcggatat
     2401 gagcccaaaa cacgcaggaa agtagcgtac tcctccaaat gggacaaggc gcgggagaca
     2461 acctcatcct gggttgaacc cgcaaaatca atgtagaaga aatactccca gggtttgcca
     2521 gccagtggtc gagattcaat cttggtcagg tcgatgtcgc gcagcgcaaa gacgctcagc
     2581 gccttgaaca aagagccagg ctcatttttc agagagaaga cgatagatgt tttcgcatcc
     2641 gctccgggat tcactggctg gcaagcgatg atttgaaagc gcgtgatgtt ggcaggatta
     2701 tcttcgatat tttcagccag gatttgcatc ccgtatagct ccgctgcctg gcgggaagcg
     2761 atcgccgcca aggtgcggtc attttgctgg cttacgatct tgacgctgcc agctgtgtca
     2821 taggcggctt ctgctttaac gtgacccagc gagcgaagtg tggcgtcaca ttgggccaca
     2881 gcctgggggt ggctgatcac ccgctgg
//
LOCUS       DBNHOH010000867         5268 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_632832, whole genome shotgun sequence.
ACCESSION   DBNHOH010000867 DBNHOH010000000
VERSION     DBNHOH010000867.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5268)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5268
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_632832"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="AC16V7_15645"
     CDS             complement(<1..204)
                     /locus_tag="AC16V7_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258605.1"
                     /translation="MNADESSTFVDWINVSLRWLLLLALTVAAAINGNLPFEVILTLL
                     VAALWNVSLTLLATINRRLWAHRY"
     gene            complement(237..848)
                     /gene="rdgB"
                     /locus_tag="AC16V7_15650"
     CDS             complement(237..848)
                     /gene="rdgB"
                     /locus_tag="AC16V7_15650"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HIR6258606.1"
                     /translation="MTKLLLASGNPGKLFEMADLLANLPIEILTPQQIELSIDVEESG
                     ATYAENAAIKAQAYARASGLLTLGDDSGLEVDALGGLPGIRSARFSPKPNATDAERRQ
                     ILLDQLMPHPRPWTARFRCTVAIATPHNELFFAQGECLGEIIPEERGENGFGYDPIFL
                     IVEYGLTMAELSTHTKNQISHRARAIQAARPILTKLITNTGET"
     gene            988..1503
                     /locus_tag="AC16V7_15655"
     CDS             988..1503
                     /locus_tag="AC16V7_15655"
                     /inference="COORDINATES: protein motif:HMM:NF014657.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIR6258607.1"
                     /translation="MPNSTDQLRLNVGFLINQSIGSSREFLFDIPVFHLDSEFHLTNL
                     GGMAKFTRIAQGLLAQVKMQATLQTECVRCLTDFQQELKIDFTELYAFSPNSITDSEL
                     LVPENAQINLRPLVREYMLLDVPINPLCSQDCKGLCSICGQNLNESTCHHEVEAVDPR
                     LSVLKSLLEKK"
     gene            1576..2757
                     /locus_tag="AC16V7_15660"
     CDS             1576..2757
                     /locus_tag="AC16V7_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR6258608.1"
                     /translation="MVYPSNASAGKQVLASLIEKANDQGYLTLDDILEADRHHGDEAT
                     IQPKKLLTYLRRQGVEIIEFDEAEINQAEPPDDLVVEFSLPTGFEKIPTEDTVEIYLK
                     EMARVPLLSVEEEIWLALRIEEGRAALSKLSQLKVNGNHRERRALENTVQDGILAREH
                     IIAANTRLVVSIAKRYIGRGVPFLDLIQEGNLGLMKAVEKYDVHRGFRFSTYATWWIR
                     QTITRAIADQGRTIRLPVHMSDRIRHMYRAAHELEQSLGRFPTAEELAERMDVPARKV
                     QWMLQVSWIPLSLESPVGDEEDAELGMFVEDQNTPTPIQVVYQNTLRERVEEVLASLS
                     PREARVLRLRFGLNEDRPYTLEEVGEKFGLTRERIRQIEGKALRRLRHPICARRLRDY
                     L"
     gene            2971..4440
                     /locus_tag="AC16V7_15665"
     CDS             2971..4440
                     /locus_tag="AC16V7_15665"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent decarboxylase
                     family protein"
                     /protein_id="HIR6258609.1"
                     /translation="MIGDKFSMDEETLDPQNWDAMRALGHRMVDDMLNYLQDIREHPV
                     WQPIPDNVKDKLRQPLPLEPQLPERVYQDFLEYVLPHPLGNIHPRFWGWVMGTGTPLG
                     MLAEMLAAGMNPNLWGTEHVANYVEAQVLDWCKAMMGFPKEASGLLVSGGSMANLIGL
                     AVARNAQAGFDIRRQGVSASPKPLVLYASMETHSSVQKAVELMGLGSQSLRKIAVNAD
                     FQIDLEALEKAIADDRAMGLNPICLVGNAGTVNTGAVDDLNSLANLAAREGMWFHVDG
                     AFGALAALSPELRPLVSGIERADSLAYDLHKWMSIPIEAGCVLVRDAEAHFNAFTLTA
                     AYLSQADRGLASGTMGYYNFGLQLTRGFRALKVWMSFKEHGIRKYGRVIQKNVEQARY
                     LANLVEEAPELELLAPVSLNIVCFRFKNDHLDDSQLDELNKELLIRLYESGIAVPSYT
                     TLRGKYALRVANVNHRSRNEDFDILVSEVIRLGKELMTS"
     gene            4437..5060
                     /locus_tag="AC16V7_15670"
     CDS             4437..5060
                     /locus_tag="AC16V7_15670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6258610.1"
                     /translation="MKLFYEILYRRFRAPWDIGPRTELVELVESGRLEPGRAIDLGSG
                     TASNCIFLAQNGFTVTGVDYAESAIDLGIQRAQEAGVNVNFIKDDLTNLRYVTGTFDL
                     LVDYGTLDDLKPSARERYLQNVLPLTRPGSKFLIYCFEWPFRWWERLLFFLFSPISEH
                     LAVEPGEIEQRFGEHFEVEKFSGQVDYSQWPPGYAVYLMTRMDGHID"
BASE COUNT         1344 a         1147 c         1411 g         1366 t
ORIGIN      
        1 ataacggtgt gcccataatc tccggttgat ggttgccagg agtgttaggg agacattcca
       61 aagcgctgca accaataaag tcagaataac ttcgaatgga aggttgccat tgatggcagc
      121 tgctaccgtt aatgccagca ataataacca gcgcaatgag acattgatcc agtcgacaaa
      181 agtcgatgac tcgtcggcgt tcataggggt tatcataatc gaaaaacaac ggcaggttaa
      241 gtctcaccag tatttgttat cagttttgtg agtattggtc gcgcggcctg aattgcccga
      301 gcacgatgac tgatttgatt ttttgtgtgg gtgcttaact cggccatcgt caagccatac
      361 tccactatta agaagatcgg gtcatagcca aaaccgttct cgcctcgctc ttctgggata
      421 atctcgccca agcactcccc ttgagcaaaa aatagctcgt tatgtggagt ggctatcgct
      481 accgtgcagc gaaagcgcgc ggtccaggga cgtggatgcg gcataagctg atcaagaagg
      541 atttgccgac gctcggcgtc ggtcgcattc ggtttggggg aaaagcgggc tgaacgtata
      601 cctggtaatc ctcccagggc atcaacttct aatccggaat cgtcccccaa agttaacaat
      661 ccggaggcgc gggcgtaggc ttgggctttg attgccgcgt tttctgcgta ggttgctcca
      721 ctctcttcaa catctatgct cagctcgatc tgttgggggg tcaggatttc aatgggcaag
      781 ttagccagca gatctgccat ctcaaaaagt tttcccggat tgccgctcgc taaaagtaat
      841 ttagtcaact tgatctccga ttgcaataaa tttttacagg caatttgatt ttgtcaaaat
      901 aataagcaaa tcttgactat taatcaattc tatgctataa tttttcaaaa ttaatagcat
      961 aattttatat cattcattcc agcatcagtg ccaaactcga ctgaccaact ccggctcaat
     1021 gtcggttttc ttattaatca atctatcggc tccagccggg aatttctttt tgatattcca
     1081 gtatttcatc tggattcgga attccatctg accaacctgg ggggtatggc gaaattcaca
     1141 cgcattgcac agggattgct ggcacaagtc aaaatgcaag ctacgcttca aaccgagtgt
     1201 gtgcgttgtc tcactgactt ccagcaagaa ctcaaaattg attttaccga attgtacgca
     1261 ttctcgccaa actctatcac ggattccgag cttttggttc ctgaaaacgc gcaaattaac
     1321 ctgagaccgc tagtacggga atacatgctg ctagatgtgc ccatcaaccc gttatgtagc
     1381 caagattgta agggactttg ctcgatttgt gggcaaaatt tgaatgaatc gacatgtcac
     1441 catgaagttg aggctgttga cccccgttta agtgtattga aatcactgct tgaaaaaaag
     1501 tgatcccctg catcacaata aggctcactt gagtagagct aaccagtaaa gctcgtcgta
     1561 ttaggaggga agcgcatggt ctatccgtcc aacgcatcag cagggaaaca ggtgcttgca
     1621 agtttaattg agaaagctaa tgatcagggg tatctcaccc tggatgatat cttggaagct
     1681 gaccggcatc atggggacga agccaccatt cagccgaaaa aattgctgac atacttgcgg
     1741 cgtcaagggg ttgagatcat cgagttcgac gaggctgaga ttaaccaggc ggaaccaccc
     1801 gatgatttgg ttgttgaatt cagtctgcca actggctttg agaaaatacc tactgaagac
     1861 accgtcgaaa tatatttgaa ggaaatggcc cgcgtgccgc tcttgagcgt agaagaagaa
     1921 atttggctgg cattacgcat tgaagagggg cgcgcagcgc tgagcaagct ttcacaatta
     1981 aaggttaacg gtaatcatcg agaacgtcgc gccttggaaa acacagtaca ggatggtatc
     2041 ttggcgcgtg agcatatcat agctgccaac acccgtttgg tggtaagcat tgcgaagagg
     2101 tatataggtc gcggcgtgcc gtttttagat ttaatccaag aaggcaattt aggcttgatg
     2161 aaagctgtag aaaagtatga tgtccatcgc ggtttccgct tttcaactta cgccacatgg
     2221 tggatccgcc aaaccatcac acgcgcgatt gccgaccagg ggcgaaccat ccgtttgcct
     2281 gtacatatgt cggaccgcat acgtcatatg taccgcgcgg ctcacgagtt agagcaatct
     2341 ttaggacgct tccccacagc tgaggaactg gctgagcgga tggacgtccc ggcccgcaaa
     2401 gtacagtgga tgctgcaggt gtcctggatt ccgctctcgc ttgagagccc agtcggtgat
     2461 gaggaagatg cagagttagg aatgtttgtt gaagatcaga acacacccac gccgatccag
     2521 gtggtgtatc agaatacact aagggagaga gtggaagaag tgctagcctc actttcgccg
     2581 cgcgaggcca gggtgctccg cttgcgcttc ggtcttaatg aagatcgtcc atacactttg
     2641 gaggaagtgg gcgaaaaatt tggtttaacg cgtgagcgaa tacgccagat tgaaggaaaa
     2701 gcgctgcggc gattgcgtca tccgatatgt gctcggcgac ttagagatta tctatagccc
     2761 gaagtgaact gcttttaata gcttggtgct aacagaggat acctgtgatg taattgtggg
     2821 tttgttcaga tgggtttaga aggattgggg ggatatttga tggagcctcg ccagaagtaa
     2881 agcgagccag acactggaat gattttgtta taatatgata caactgatct gattagctgg
     2941 aatttatgca ttgtctgaaa gaggaaatct atgatcgggg ataaattttc tatggatgag
     3001 gagacgcttg accctcaaaa ttgggatgca atgcgggcat tagggcatcg catggtggat
     3061 gacatgctta actatttaca ggacattcgt gagcatcctg tgtggcagcc tatcccagac
     3121 aatgtgaagg ataagctgag acaaccgctc ccactggagc cgcagttgcc agagcgggtt
     3181 taccaggatt ttctcgagta cgtgctccct cacccattgg gtaatattca cccgcgcttt
     3241 tggggttggg tgatgggtac gggtacacca ctgggcatgc tggctgagat gctggcggct
     3301 gggatgaacc caaacctgtg gggtacagag catgtggcaa actatgtcga ggcgcaggtt
     3361 ctcgactggt gcaaagcgat gatgggcttt cccaaggagg caagcggact gctggttagt
     3421 ggtggttcga tggctaacct gattggactc gcggtcgcgc gtaatgccca ggcaggcttc
     3481 gatattcgtc gccagggagt gagcgcctca ccaaaaccat tggttctcta cgcctcaatg
     3541 gagacgcaca gctcagtgca aaaagcggtc gaactgatgg ggttagggag ccagtcgctg
     3601 cgcaagatag cggttaatgc tgattttcaa attgacttgg aagcattaga aaaggcgatt
     3661 gcagacgacc gagcgatggg tttgaatcca atttgtttgg tgggcaacgc tggtacggtg
     3721 aacacgggtg ctgttgatga cttgaacagc ctggcaaact tagccgcgcg cgaagggatg
     3781 tggttccatg tggatggtgc attcggtgcg ttggcggctc tctctcctga actacgcccg
     3841 ctggtttcgg ggatcgaacg agccgattca ttagcttatg atctgcacaa gtggatgtct
     3901 attcctattg aggcgggatg tgttctggta cgggatgcgg aggcgcattt taatgcgttt
     3961 acgctaacag cagcttacct ctcgcaggcg gatagaggac ttgcctcagg gacaatggga
     4021 tactataact ttggcttgca actgacacgc ggattccgcg ccctgaaagt atggatgtct
     4081 tttaaggaac atggcattcg aaagtacggg cgagtgatcc aaaagaatgt ggagcaggca
     4141 cgctacctgg caaacttggt ggaagaagcg ccagaattgg agcttttggc tcctgtgtcg
     4201 ttaaatattg tatgtttccg ttttaaaaat gatcacctgg atgattctca actcgatgag
     4261 ttgaacaaag agttgctgat ccgactttat gagagtggaa tcgcagtacc tagctatacg
     4321 acgctgcgcg ggaaatatgc cttgcgggtc gctaatgtga accatcgcag ccgtaatgag
     4381 gacttcgata tcttggtaag cgaagttatc cgtttgggta aggagttgat gacatcgtga
     4441 aattatttta tgaaatcttg tatcgccgct ttcgggctcc ttgggatatt ggaccacgca
     4501 ctgaattggt ggaactggta gaaagtgggc gtcttgagcc aggcagagcc attgaccttg
     4561 gtagtggtac agccagcaat tgcatatttt tagctcaaaa tgggtttacc gtgacaggcg
     4621 tggactacgc tgagtcagca atcgatttgg gtatacagcg tgctcaggag gctggtgtca
     4681 acgtgaattt catcaaggat gacctgacta atctgcgcta tgtaaccggc acattcgatc
     4741 tgttagttga ctatggaacg cttgatgacc taaagccatc agctcgcgag cgctatctgc
     4801 agaatgttct tcccttgacg cgtcccggca gcaaatttct tatttactgt tttgagtggc
     4861 ccttccgatg gtgggaacgc ttgttgttct tccttttctc tccaatttct gagcacttgg
     4921 cggtcgagcc gggtgagatc gagcagcgtt ttggggaaca tttcgaggta gagaaattct
     4981 ctggtcaggt tgattattcg caatggcctc ctggttacgc tgtttattta atgaccagaa
     5041 tggatgggca tatagactga ttggaaaaat gttatcactc accctgctgg cttcgattaa
     5101 agagtgttca ctttataaca acaaacgagg ctgtgaacac gaggcgaaca cacgaggcga
     5161 acactgtcat tgttcaggga aaatgtcact ctaactgttc agcaaaaatg tcaccttgac
     5221 caatggttgt gtgtaagtat aatctgaacc atcccaaagg aaaggcga
//
LOCUS       DBNHOH010000868         3626 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_55500, whole genome shotgun sequence.
ACCESSION   DBNHOH010000868 DBNHOH010000000
VERSION     DBNHOH010000868.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_55500"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="AC16V7_15675"
     CDS             <1..459
                     /locus_tag="AC16V7_15675"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6258611.1"
                     /translation="IIGYLRNLEPVHHEIPPISANIVAKVMMAMGAFGPSSVGEPITA
                     PKDTPPAGTPEYGQYMLALGACSDCHKQNLAGGPIPFAEEGMPWSANLTPAGELANWS
                     EADFIAAVSEGRHPGGGNLHDEMPRYKMNDEDLKAIFMYLKTIPPAQPEK"
     gene            534..1235
                     /locus_tag="AC16V7_15680"
     CDS             534..1235
                     /locus_tag="AC16V7_15680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258612.1"
                     /translation="MLGHNLRQIAVRSALVTALFLFGGFLLGFAAAIGFSQLPMHVPD
                     SLQMLVAMAIILSVLFVAGAAWGRAMARLSGFAETRRMAKAGAVSFGPALVLSALVLS
                     SLEVAIIERGGGTSLPIHVVVTILFVPAVFIVAGVGGLALGIAQGDWRFAWRCALSSG
                     LAGSAAFLLVNLGMDQLGWRVGAPGAAERATMLTVMMAGSLGAALAGGGAIGGMLGKN
                     ALRGVSIALEVDQYN"
     gene            1292..2203
                     /locus_tag="AC16V7_15685"
     CDS             1292..2203
                     /locus_tag="AC16V7_15685"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6258613.1"
                     /translation="MKIRLGKLFQWIGILLIVLLGLALLALLALYYTSNQKLNKTYEV
                     SFEPLEISMDPETIERGRRIVTTRGGCIECHGGNFAGDIIDEGFLMAKLVVPNLTPGK
                     GGVGASFTDDDWVRAIRYGIDSDGTSVIIMPSDYFNVLSDADLAAIIAYLRSLPPVDT
                     NYPKIRLGPMGRWIVLQEPHLLPASVIDHSAPRPPDPVPGVTVEYGKYLSKICTACHA
                     ENLAGGEEPGEGLNLTPAGDLGEWSEAQYIRAMRTGVTPSGKQLDPELMPWKSLGQMS
                     DDELKAIWLYLQTVPPVENLPQPTSSP"
     gene            2220..3506
                     /locus_tag="AC16V7_15690"
     CDS             2220..3506
                     /locus_tag="AC16V7_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258614.1"
                     /translation="MSVEKQIDRLPDSSQRLSFARLVWVFIFLLIPTLFLVGSPSRLE
                     YFTRFADQRSLYMLGMDASTYGLLAVILDLIFILAHHTIALVIFLRRNDDGMALLVSL
                     ALIANGAMIPLALTYSQLQANPIVHGLVNLVIYLSLVSSILLLYAFPDGRFRPDWTRW
                     MAILWALLCLPAIFAPELPISLPSWPTPLQVIVLLSVSGTGVFAQVYRYLNVSSPLQQ
                     QQAKWAVFGLTAASLGPFVYFLAFVILPILGQQSVSNMLYQRMGSSFFSFSYITRLIN
                     SAGFNLFAMVFPLSFAIAILRYRLWDIDILINRALVYTALSGTLLTFYLGSVVLLESI
                     LRPIIGAGSNQLVTVISTLVIAASFAPLRRRVQNLIDRRFYRSRYNAARTIENFGVGL
                     REEVDLSTLSERLVAVVDETMQPEFVNLWMRKGEGK"
BASE COUNT          711 a          945 c         1031 g          939 t
ORIGIN      
        1 attatcggtt acttgcgcaa tttggagccg gtgcaccacg agatcccacc tatctccgcc
       61 aacattgtcg ccaaggtgat gatggcgatg ggcgcttttg gccccagttc agttggcgag
      121 ccgattacag ccccaaagga tacccctccg gctggcacgc ctgaatacgg tcaatatatg
      181 cttgccttgg gcgcctgctc agactgccac aaacagaatt tggcaggtgg tcctattccc
      241 tttgccgaag aaggtatgcc atggtctgct aatctgaccc cagcgggcga gcttgccaat
      301 tggagcgagg ctgattttat tgccgctgtg agtgaagggc gccatccagg cgggggcaat
      361 ctccatgatg agatgccgcg ctataaaatg aacgatgaag acctgaaagc cattttcatg
      421 tatctaaaaa ctatcccgcc agcgcagcca gagaagtagt taacagctac cagggggttg
      481 gggatttgct tccccaaccc ttctgggaca actccaggag acaagagtta aaaatgttgg
      541 ggcataattt gcgccagatc gccgtccgca gtgccttggt tactgcgctg tttttatttg
      601 gcggttttct attgggcttt gcggctgcga tagggtttag ccagctacct atgcatgtgc
      661 ccgactcgct gcagatgctt gtcgctatgg cgattatact gagtgtcctg tttgtcgcag
      721 gtgcagcctg gggtcgagcc atggcgcgcc tcagtggatt cgctgagacg cggcgtatgg
      781 caaaggcggg tgcggtgagt tttggtccgg cgctggtctt gagtgcattg gtcttgtcct
      841 ccctggaagt cgccattatc gagcgcggag gcggcacatc gctaccaatc catgttgtcg
      901 tcaccatcct ctttgtgccg gcggtcttca ttgtagccgg cgtgggaggt ttggctttgg
      961 gtattgccca gggcgattgg cgatttgcgt ggcgctgcgc attgagcagt ggacttgccg
     1021 gaagcgcggc cttcttgctt gtaaacctgg ggatggacca gcttggctgg cgagtgggtg
     1081 cacccggcgc ggcggagcgg gctaccatgc tgacggtgat gatggctggc agtcttggcg
     1141 cagcgctggc gggcggtgga gccatcggag gcatgctggg taaaaatgct ctccgcggtg
     1201 tttccatcgc gcttgaagtt gatcagtata attaaccggt acgattaata acaactagat
     1261 ggtggtagaa aactattcta tgaggtgaaa agtgaaaatc aggcttggga aattatttca
     1321 atggatcgga atcctgttaa tcgtcttatt gggattagcc ttgctggcct tgcttgccct
     1381 gtactacacc agcaatcaaa aactgaacaa gacctacgag gtttcgtttg aaccgttgga
     1441 aatttcaatg gatccagaga ccatcgagcg cgggcgccgt attgtgacga ctcgaggtgg
     1501 gtgcatcgaa tgtcacggag gcaatttcgc tggggatatc atcgatgaag gctttttaat
     1561 ggccaagctg gttgtcccca acttgactcc cggcaagggt ggtgttggcg cgagctttac
     1621 cgatgacgat tgggtacgcg ctatccgtta cggtatagat tccgatggca cgtctgtaat
     1681 catcatgccc tccgattatt tcaatgtcct cagcgacgct gacctggccg cgatcattgc
     1741 ctacctgcgc agtttgccac ccgtagacac aaattaccca aaaatccgct tggggccgat
     1801 gggtcgctgg attgtgttac aagaacctca tttattgccg gccagcgtga tcgaccacag
     1861 cgccccgcgc cctcccgatc ctgtacccgg tgtaaccgtg gagtacggaa aatacctctc
     1921 caaaatttgc acggcctgcc atgctgaaaa tctggctggc ggagaagagc cgggtgaggg
     1981 gctcaacctc acccctgctg gcgacctggg cgagtggagc gaggcgcaat atatccgcgc
     2041 catgcgcacc ggcgtgaccc cttccggtaa gcagctagac ccggaattaa tgccctggaa
     2101 atctttaggg cagatgagcg acgacgagct caaagccatc tggctatatt tgcagactgt
     2161 gccgcctgtg gagaatctgc cgcagccaac atcttctcct tgatacatag gaattaccga
     2221 tgagtgttga aaaacaaatc gatcgactcc cggactcgag ccagaggctt tctttcgccc
     2281 gtctcgtctg ggtgtttatt ttcctgctga ttcccacgct atttctggtg ggcagtccta
     2341 gcaggctgga gtatttcacc cgctttgcgg accagcgatc gctctatatg ctggggatgg
     2401 atgcttcgac ttatggtctg ctggcggtca ttcttgattt gatcttcatc ctggcgcacc
     2461 acaccattgc cttggtgatc ttcctgcgcc gtaatgatga cggtatggcg ctcctggtgt
     2521 cccttgccct gatcgccaat ggtgccatga tcccgctggc attgacatac agccaattgc
     2581 aggctaatcc aatcgtgcac ggtctggtaa acctggtcat ctaccttagc ctggtctctt
     2641 ctattctgtt gctgtatgcg tttccagatg ggcggtttag gcctgattgg actcgctgga
     2701 tggcgatcct gtgggcgctg ctatgcctgc cggccatctt cgccccagag cttcccatca
     2761 gtctacccag ctggcctact ccacttcagg tcattgttct actcagcgtg tctggtacgg
     2821 gggtttttgc ccaggtatat cgctatctaa atgtctccag cccgctccag cagcagcagg
     2881 cgaagtgggc agttttcggc cttaccgcag ccagcttggg gccgtttgtc tatttcctcg
     2941 ccttcgtcat cttgcccata ctgggtcagc agtctgtgtc taatatgctt taccagagga
     3001 tgggatcttc gtttttctct ttctcgtata tcacgcgtct gatcaattcg gccgggttta
     3061 acctgttcgc catggtcttt ccgctgtctt ttgccattgc catcttgcgt taccggctgt
     3121 gggatatcga tatcctgatc aaccgggcgc tggtctacac agccctctcg ggcaccttgc
     3181 tgacctttta tctgggcagt gtggtattgc tggagagcat cctgcggcct atcatcggtg
     3241 cgggtagcaa ccagcttgtc acggtcatct ccaccctggt gattgcggct tcttttgccc
     3301 cactccggcg cagagtacaa aacttgattg accgccgttt ctatcgcagt cgttataacg
     3361 ccgctcgtac gatcgagaac ttcggcgttg ggttgcggga agaggtggat ttgagcactt
     3421 tgagtgagcg gttggtagcg gttgttgatg aaaccatgca gccagaattt gttaacctgt
     3481 ggatgcgcaa gggtgaggga aaatgagcgc attgtaggca acgatcctca aggagcgtaa
     3541 ccgggcttcg atccgggtgg ccgaaaaaac cgggatggcc ttcgagaagg aaggtcgcga
     3601 cgaactgggg ccttttcatt tgtatg
//
LOCUS       DBNHOH010000869         3837 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_119565, whole genome shotgun sequence.
ACCESSION   DBNHOH010000869 DBNHOH010000000
VERSION     DBNHOH010000869.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_119565"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1596
                     /locus_tag="AC16V7_15695"
     CDS             136..1596
                     /locus_tag="AC16V7_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HIR6258615.1"
                     /translation="MPIHFGTDGWRAVISDTFTYTNLRMVAQAIADAISSAQWNNETL
                     DNGNPDSRDIIVGFDTRFLSDRYAGEVARVLAANGYTVHLAQADAPTPAISYNLKHLG
                     ATAGIMITASHNAPRYNGVKLKAAYGGSALSEQCRRVEVYLNDNEERARGPNLMDYEQ
                     ARQLGLIRRFNPLPAYYEHLRKLIDFDLIAENPQRVVVDSMYGSGRGVLKGVLQGTGC
                     EVQEIRGEMNPGFGGVHPEPIAKYLGALAGAISTHMGNFGLATDGDADRIGAMDERGN
                     FVDPHKIMALGLRHLVERRGWRGPVVRTVSTTRMIDRLAKQYGVPVYETPVGFNHIAN
                     YMLKEDVLIGGEESGGISFRGHIPEGDGILMGLLIIEMVAASGGSLYELVEELLRQAG
                     PIQYQRNDLRLKQPVPKDRMNQRLVENAPAQIAGENVLEVSTLDGVKYILADDSWLLI
                     RPSGTEPVLRVYAEGRTAEMVRSMIGFGEQVAASFT"
     gene            complement(1600..2139)
                     /locus_tag="AC16V7_15700"
     CDS             complement(1600..2139)
                     /locus_tag="AC16V7_15700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIR6258616.1"
                     /translation="MEELDTNNIEFPGEEIPPPEESSNRTFMIVAGILGGITVLALAC
                     IAAYAFLVLPRQRAQQEAQKATLTAQANEVAQALTSTAIAAAIVPTSTNTPLPTATLA
                     RTATPLLAMATNTPVPSSDPRTATVAALLTQAAAVTQATSTALPSTGFADEVGMPGLL
                     GMAVLLIVVIFLARRLRTA"
     gene            complement(2188..3243)
                     /locus_tag="AC16V7_15705"
     CDS             complement(2188..3243)
                     /locus_tag="AC16V7_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258617.1"
                     /translation="MLAPVVHILPLTRIRRDRLLPVPGKVLVRRGQMVSTTDVIAESN
                     LNPEHLLLNIARGLGLPEEKADQYLQCKAGMRVSQGDVLAGPVGMAKRVVRAPKNGNV
                     IVAGGGQVLLELESPPYEVKAGMPGTVVHLMDELGAAIETTGALIQGVWGNGRLDFGL
                     MYVLMKNPDDVFTPDRLDVSLRGSIILGGYCDNPEVLKSAASLPMRAMIFSSMAAALI
                     PLAERIRIPILVIEGFGQHPMNSAAFKLLSTNERREVAVNAQPWDPFTGNRPEIVIPL
                     PANVDPPLPQDTEPFTAGQQVRVIRAPHQGSIGTIVELRPGMSELPNGISAAVAEIQL
                     ESGEKALLPLANLEVLA"
     gene            complement(3320..>3837)
                     /locus_tag="AC16V7_15710"
     CDS             complement(3320..>3837)
                     /locus_tag="AC16V7_15710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258618.1"
                     /translation="TRAPNLPQTMLMLLDGLQPTGVTTLVLDQNHLAPSLGAVAAINP
                     LMVVQVLESNTFLTLGSVISPVGVARPGTPILRIRMIYEGGAETSLEVKQGSIEMLQL
                     PTGQTAQLHIQPLHRFDVGMGGPGRGGRLRVVGGALGVVIDARGRPLYLPNDPALRRE
                     LIAKWRWMVGC"
BASE COUNT          872 a          981 c         1116 g          868 t
ORIGIN      
        1 cccccaaaac tcccccctca cctctagaac tgcgtaactt atgaaacgat tatatttaaa
       61 ccagccttgc taatatgggc ggcataggat cgatagcctt tcgcaaggct gaatttacac
      121 agcaggagac aatatatgcc gatacatttt ggcaccgatg gctggcgcgc cgtcatttct
      181 gataccttta catataccaa cttgcgcatg gttgctcaag ccatcgccga cgcgatcagt
      241 tcagctcagt ggaacaatga gactttggat aacgggaatc cggattcgcg agatattatc
      301 gttggcttcg acacacgctt cctttctgat cgatacgcag gggaggtggc gcgcgtactg
      361 gcagcaaacg gctacacagt ccatctggcc caagcagatg cccctacacc ggcaatctcg
      421 tataacctta aacacctggg tgcgactgct gggatcatga tcacagcctc gcacaacgct
      481 ccgcgttata acggtgtcaa gctcaaggcg gcttatggag gttcggcgct tagcgaacag
      541 tgccgccgtg tggaagttta tctgaatgat aacgaagagc gtgccagagg tccaaacttg
      601 atggactacg aacaggcgcg ccagttgggg ctgatccggc gctttaaccc gctgccagcc
      661 tattacgaac acctgcgaaa gttgatcgac ttcgatctaa ttgccgagaa cccgcagcgc
      721 gtggtagttg attcgatgta tgggtcgggg aggggagtgt taaagggtgt cctgcaaggc
      781 acaggttgcg aggtacagga gattcgcggt gaaatgaatc ccggatttgg cggcgtgcac
      841 ccagagccca tcgcaaaata cctgggggcg ctggctgggg caatcagcac acacatgggc
      901 aatttcggct tagccactga tggagacgct gatcggattg gggcgatgga tgagcgtggg
      961 aattttgtcg accctcataa aattatggcg ctgggattgc gtcatctggt agagaggcgc
     1021 ggatggcgcg gtcctgtagt gcgcaccgtg tctactacgc ggatgattga ccggttggca
     1081 aaacaatacg gcgtcccggt atacgagacg ccggtgggtt ttaaccatat cgccaattat
     1141 atgctgaagg aggatgtcct tatcgggggt gaggagtctg gagggatctc ttttcggggt
     1201 cacatcccag agggtgatgg tatcctgatg ggcttgttga ttatcgagat ggttgctgcc
     1261 tcgggtggta gcctgtatga attggttgaa gaattactcc gtcaagccgg gccgattcaa
     1321 taccagcgaa acgatctgcg cttgaaacaa ccggtaccca aagatcgcat gaaccagcgc
     1381 ctcgttgaga acgcgcctgc ccaaattgca ggtgagaatg tcttagaggt ctccacactg
     1441 gatggcgtga aatacatcct ggccgacgac tcctggttgt taattcgtcc ttctgggaca
     1501 gaacccgttt tgcgggtata tgccgaaggg cgcaccgcgg agatggtgcg gagcatgatt
     1561 ggctttggcg agcaggtggc agccagcttt acctaataat tatgcggtgc gcagccgccg
     1621 agccaggaag atcacgacga tcaacagaac cgccatgccg agcaagcctg gcatgcctac
     1681 ttcatcagcg aaacccgtgc tgggcaaggc agtcgaagtg gcttgcgtta cggctgcggc
     1741 ttgcgtcagt agcgcggcca cggttgctgt acgcggatct gaagagggca cgggtgtgtt
     1801 ggtggccatt gccagcagcg gagtggctgt gcgcgccagc gtcgctgtag ggagcggcgt
     1861 attggttgat gtggggacga tggcagcagc gatggctgtg gatgtcaatg cctgagccac
     1921 ctcgttagcc tgtgcggtca atgtagcttt ttgggcttcc tgttgggccc gttggcgcgg
     1981 caaaacgagg aacgcatatg ctgcgatgca ggcgagcgcc aaaacggtaa tcccacctaa
     2041 gataccagct acaatcataa atgtccggtt gctggattcc tcggggggtg gtatttcctc
     2101 gccaggaaat tcgatgttgt tagtatccag ttcttccata acactctcct cctaagcttt
     2161 atccgggatg gtttatcgga tattcattta tgctaatacc tctaaatttg ccagcggcaa
     2221 gagtgctttt tcgccgcttt ccagttggat ctcggctaca gcagctgaga tgccattggg
     2281 taattcagac atacccgggc ggagttccac aatcgtaccg atgctgcctt ggtgaggggc
     2341 gcggatcacg cgcacctgtt gcccggcggt aaatggttcg gtatcttgag gcaggggtgg
     2401 gtctacattg gctggcaagg gtatgacgat ctcgggtctg ttcccggtga atggatccca
     2461 gggttgagca ttgacggcta cttcacggcg ttcattcgtc gagagcaatt tgaaagctgc
     2521 cgagttcatc gggtgctgcc cgaagccttc gattaccagg attgggatac gaatacgctc
     2581 cgcaagcggt attaaggcgg cagccatgct ggaaaagatc atggcgcgca taggcaagct
     2641 cgcagcggac tttaatactt ccggattatc acagtagccg cctagaatga tagacccgcg
     2701 caagctaaca tccagtcgat cgggggtgaa tacgtcgtct ggattcttca tcagcacata
     2761 catcaaacca aaatcaagac gcccgtttcc ccagacacct tgaattaacg ccccggttgt
     2821 ttctatagcg gcgcccaact catccatcag gtgcaccaca gttcccggca taccggcttt
     2881 gacctcgtat ggaggtgatt ctagctctag caaaacttgc ccgcctcctg ctacaataac
     2941 gttgccgttt ttaggggcac gcaccactcg ttttgccatt ccaaccggac cggctagcac
     3001 atctccttgg gaaacgcgca tccccgcctt acattgaagg tactgatctg ctttttcttc
     3061 gggcaaaccc aagccgcgag ctatattcag gagcaaatgt tcaggattca ggttggattc
     3121 agcaatcaca tcagtggtag ataccatctg gccgcggcga acgagcactt tgccggggac
     3181 cggcaagagg cggtcgcgac gaatgcgtgt cagagggagg atatggacaa caggtgctaa
     3241 catagactca atgcctcatg aagaggggtg caacagtcca tcgaccagct ttgttgcaag
     3301 gagagtgcca acctaatcat cagcagccta ccatccagcg ccatttggcg attaattccc
     3361 gccggagggc aggatcattg ggtaaataca atgggcgtcc gcgcgcatct ataacaacgc
     3421 cgagcgctcc accaaccacg cgcaatcggc cgccgcggcc ggggcccccc atccccacgt
     3481 caaagcggtg taatggctgg atgtgcaatt gggcggtctg cccagtgggc agctggagca
     3541 tctcgataga accttgttta acctcaaggc ttgtctcggc accgccttca tagatcatgc
     3601 ggatacgaag gatgggtgtg cctggacgag ctacgccaac cggggagatc actgagccta
     3661 gggtgaggaa ggtattggat tctaatactt gcacaaccat cagtgggttg attgctgcca
     3721 ctgcgccgag tgatggcgca aggtggtttt ggtccaaaac cagggtggtg acaccggtgg
     3781 gttgcaaacc atccagcagc attagcatgg tttgaggtag attgggtgca cgagtga
//
LOCUS       DBNHOH010000870         3709 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_119623, whole genome shotgun sequence.
ACCESSION   DBNHOH010000870 DBNHOH010000000
VERSION     DBNHOH010000870.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_119623"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="AC16V7_15715"
     CDS             complement(<1..404)
                     /locus_tag="AC16V7_15715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258619.1"
                     /translation="MARWGKLLIVLVLVLTMVPVLPALAQTGIVCDYYGVHGNGAKYC
                     ITLPSEGDWNGDLVIFAHGYVSVREPLEIPWDQLILPDGTPMPAIVNNLGYAFATTSY
                     SENGLAVVRGVIDILDLVEVFNDTVGRPNRVYL"
     gene            complement(684..977)
                     /locus_tag="AC16V7_15720"
     CDS             complement(684..977)
                     /locus_tag="AC16V7_15720"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HIR6258620.1"
                     /translation="MAETHNARLHAIVEGYVQGVGFRYFVQENAVTLGLRGWVRNLWS
                     GDVELVAEGDRQALDKLIAALRRGPPTAHVSGVRTEWGDFTGEFRSFFVKSTI"
     gene            complement(961..2166)
                     /locus_tag="AC16V7_15725"
     CDS             complement(961..2166)
                     /locus_tag="AC16V7_15725"
                     /inference="COORDINATES: protein motif:HMM:NF024876.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HIR6258621.1"
                     /translation="MGKKTIFFILAIILAGNLYVYPVRAQAGEILTSVQYTFGEEITF
                     RGEIPAGVSVESATLFFVAEGELQTRTGAATWDNRGQLLYSADLTQSPMRAFSTVVYW
                     FEVTESGGRVLKSERGSFYYEDNRFEWRTRQSKDFRVHWYEGDTAFAQSLLDVAELGL
                     TNIQAVLPLTLSSPVDIYAYATAQEMRATLQNLGKNWVGAHTDPDLGVMVVSLPRGPE
                     QRLEMERQIPHELMHLMLYQWLAVGYGSIPAWLNEGLASIAELYPNPDYLILLNKALD
                     EDSMLPIVSLCDTFPRDASGAFLAYAEATSFTRFLYQQYGTTGLEALTRAYADGMSCE
                     RGVQTALGSTLTQLERQWRREAFREDALLKAFRNLSPWLIILVLALIVPLGLTLAGTR
                     RKSKDGRDT"
     gene            complement(2171..3082)
                     /locus_tag="AC16V7_15730"
     CDS             complement(2171..3082)
                     /locus_tag="AC16V7_15730"
                     /inference="COORDINATES: protein motif:HMM:NF015509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIR6258622.1"
                     /translation="MIDLLTLFANNILPIFLAAGSGYLLSKYLKVIPRTISQVGFYIF
                     SPCLVFMLLTNSQLSGSAILQMSAFTVVSMLAVGMLAWLLGRLLRLERRMLVAVIMGA
                     MFGNSGNFGLSMNMFAFGETALAHASVYFVTSAMLTYTVGVVLASLGKSGLKEAVVGL
                     FKVPGMYAVIVAMIFNGYGWKLPLPLDRTVTLLGNAAIPILMVLIGVQLERAKWDGQP
                     VALGLTNIMRLVVAPVVAVGFTLLFGLRGAALQAAISESGTPTAVLMTVLATEYDVEP
                     SFVTTAVFTSTLLSPLTLTPLLAMLGA"
     gene            complement(3079..>3709)
                     /locus_tag="AC16V7_15735"
     CDS             complement(3079..>3709)
                     /locus_tag="AC16V7_15735"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR6258623.1"
                     /translation="LGHYIYLGEIDELPASTLHQLEDKLLQVTSGKDSSLVRRRALEA
                     LGFSDREEVPVLIKAAYNSGKNDWLSSALFAMGRSVNRAWNKAVISKLDSDVPGVLSE
                     AARAAGELEIKEAVPRLMQLVEDGDSDVRWAAVWALSQIGGEGVRERLEKLYKQTDDD
                     EEADFIESALDNLDFTEDTSLFDLLDIEEDELDDEFYFDLREDEEDDKD"
BASE COUNT          995 a         1052 c          839 g          823 t
ORIGIN      
        1 aggtagaccc ggttggggcg gccgacagta tcgttgaaca cctccaccag atcgaggatg
       61 tcaattactc cccgcacgac cgccagccca ttctcgctgt agctcgtggt ggcaaatgca
      121 tatcctaaat tgtttacaat ggcaggcatt ggagtgccat ccggcaagat cagctggtcc
      181 catgggatct ccaatggctc tctgacagaa acatacccgt gcgcaaaaat gactagatcg
      241 ccattccaat caccttcaga gggcaacgtg atgcaatatt tggctccatt gccatgcact
      301 ccatagtagt cacatacaat acctgtttgc gccaatgcag gcaagacggg gaccatagta
      361 aggaccagga caaggactat taacagtttt ccccaccgag ccatgataga cctcctttta
      421 caaatcagtc atactatttg aatatctgat aatcaccgcc ctacaaataa aaaattcaag
      481 cccagcaata acataactac cgttggtcag tgctataaga acgaagcaca aaccctgtcg
      541 cgaaaaaaat acccagttaa ttatgctatg ctataaatca ttatagcaat ttttttatgt
      601 aattcaaccc tcgaaaaggg taaatcgaga atttcacaag aaggcaatct ttggagagtg
      661 gaagccgttg gcaggtttga ccgtcagata gtcgatttaa cgaaaaaact cctgaactcc
      721 cctgtaaaat ccccccactc tgtgcgtact ccgctcacgt gggcggtggg cggcccgcgg
      781 cgcaaggcgg cgatcagttt atccaacgcc tggcggtctc cttctgccac taattcgaca
      841 tccccgctcc aaagattgcg cacccaaccg cgcagcccca gggtaaccgc attttcctga
      901 acaaaatagc gaaagcctac cccctgcaca tagccttcga cgattgcgtg aagccgggcg
      961 ttatgtgtct ctgccatctt ttgatttcct tctcgtcccc gccagggtca aacccagggg
     1021 tacaatgagt gcaagaacca aaataattaa ccagggggat aggtttcgaa atgcctttag
     1081 caaggcatct tcccgaaagg cctctcgccg ccactgccgc tccaactgag tgagggtact
     1141 gcccagcgcc gtctgcacac ccctttcaca ggacataccg tcggcataag ctctggtgag
     1201 agcttccaac cccgtagtcc catactgctg atacaagaaa cgggtaaatg atgtagcctc
     1261 ggcataagcc aggaaagcgc cagatgcatc tcttggaaat gtatcacata gggaaacgat
     1321 aggcagcatg ctatcctcgt ccagcgcttt gtttagcaag atcaagtaat ctggattggg
     1381 gtatagctca gctatcgatg ccagtccttc gtttaaccag gccgggatgc tgccatatcc
     1441 tacagccagc cattggtaga gcatcaggtg catcaattcg tgagggatct ggcgctccat
     1501 ctccagacgt tgttcgggtc cgcgcggcag cgagaccacc atcacaccca gatccggatc
     1561 ggtatgtgct cccacccaat tcttccccag attctggagc gtagcgcgca tctcctgggc
     1621 agttgcatag gcatagatat ctactggtga gctcaatgtc aagggcaaga cagcctggat
     1681 atttgtcagc ccaagctctg ccacatccag caggctctgg gcgaaagcag tatctccctc
     1741 ataccaatga acgcggaagt ccttcgattg gcgcgtgcgc cactcaaagc ggttgtcctc
     1801 atagtagaaa gaacctcgct cgctcttcaa tacccttccc ccgctctcgg tgacctcaaa
     1861 ccagtaaacg accgtagaga aagctcgcat aggcgattga gtcagatcag cagagtagag
     1921 cagttgccca cggttatccc acgtagccgc gcctgtacga gtttgcaact cgccttcagc
     1981 cacaaagaac agggtagctg actcgacaga tacaccagct gggatttctc cacggaaggt
     2041 gatctcttcc ccaaaggtgt actgcacact ggtaagaatt tcgccagcct gggcgcgaac
     2101 cgggtacaca tacaggttgc ctgccaggat aattgccaaa atgaaaaata tcgtcttttt
     2161 ccccatcaaa ttatgctcct agcatggcca aaagaggcgt caaggtcagc ggactgagca
     2221 aagtgctggt gaacactgct gtagtgacaa aggatggctc aacatcgtac tctgtcgcca
     2281 gaactgtcat gagaactgca gtcggtgtcc cagattcgga gatggcagct tgcagcgcag
     2341 ccccgcgcag tccaaataac agcgtaaaac cgactgcaac tactggtgct accaccaaac
     2401 gcatgatatt ggttaatccc agggctacag gctgcccatc ccacttggct cgttcgagct
     2461 ggacaccgat caacaccatt aaaatcggga tagcggcatt acccaataaa gttacagtcc
     2521 gatctagcgg caatggcagc ttccacccat atccattgaa aatcatcgcc acaataacag
     2581 catacatgcc tggtaccttg aagagaccca ccacagcttc cttcaagcca gatttgccta
     2641 gagaggccag caccacaccc actgtgtagg tcagcatagc cgaggtcaca aaataaacgc
     2701 tggcatgcgc taatgccgtc tctccaaagg caaacatgtt catggaaagg ccaaaattac
     2761 ccgagttgcc gaacatggct cccataatca cagctaccag catacggcgt tccagcctca
     2821 gcaaccggcc cagcaaccag gctagcatgc ccacagctaa catacttaca accgtaaatg
     2881 cagacatctg caggatggca ctgccactga gctgactgtt ggtcagcagc atgaatacca
     2941 ggcaaggact gaagatataa aaaccaactt gagatatagt acgtggaatt actttaagat
     3001 actttgataa taaataacct gagcccgcag ccagaaaaat gggtaggatg ttgttggcga
     3061 acagggtaag caggtcgatc aatctttgtc gtcctcttcg tcctcacgca agtcgaaata
     3121 aaactcatcg tccaactcat cttcctcgat atccagcaag tcgaaaagtg aggtgtcttc
     3181 agtgaaatcc aggttatcga gggcagattc gataaagtcc gcctcttcgt cgtcatcggt
     3241 ctgcttatat agcttttcga gtctctcccg caccccttcg cctccaattt gtgaaagcgc
     3301 ccatacggct gcccaacgca catcggagtc cccatcttct accagctgca tcaaacgggg
     3361 aacggcttct ttgatctcca actcgccagc ggcgcgcgcc gcttcactca aaacccccgg
     3421 cacatcgctg tccagcttgg aaatcactgc tttattccag gctctattga ccgatcgtcc
     3481 catagcgaac aaagcgctgg acaaccagtc gtttttaccc gagttatagg cagctttgat
     3541 caacactggg acctcttctc ggtcggaaaa tcccagtgct tctaaggcac ggcgccgtac
     3601 taaactagaa tccttgccgc ttgttacctg gagcaattta tcctcaagct gatgcagcgt
     3661 ggatgccggt aattcatcaa tctcccctaa atagatataa tggcctaag
//
LOCUS       DBNHOH010000871         3563 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_697377, whole genome shotgun sequence.
ACCESSION   DBNHOH010000871 DBNHOH010000000
VERSION     DBNHOH010000871.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_697377"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="AC16V7_15740"
     CDS             <1..1002
                     /locus_tag="AC16V7_15740"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR6258624.1"
                     /translation="LTSLSCNLNGSFEHEEPALASPTPVQAELATNTPEASPTTAKTQ
                     APAQPTQTREPTTLPPTNTPTQAVVQIGPCEGEVCLVPFQFPFSRPISPPGRDFVDIS
                     YRFGSSDHGKRDPHHGVEFLNSHGTPVFAAADGEVLVAGDDTKVKLGLYPNMYGNLVV
                     LQHEVPGFEPPIFTLYAHLSQVDVQVGDRVEAGDKLGQVGASGAAIGSHLHFEVRYGE
                     NTYDASRNPELWLKPLMDGSGQPMGALAGRILDGKDQPLKVANIVVESLSGSTYYLNT
                     YSETKLVGLDPWQESFAIGDLPAGEYQISFVQGGIQQRTIEVQPGVLTLVTFRIGGQ"
     gene            1013..2758
                     /locus_tag="AC16V7_15745"
     CDS             1013..2758
                     /locus_tag="AC16V7_15745"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HIR6258625.1"
                     /translation="MNHEQALKVAMIFRVSALRASFLGLSVILASCKAAAPLVPLLQT
                     GLSQPTITYTPFQPLPPSAMPTTPPPPTATAVPTAPAGLTLWVDPSLPDALRSAVKPP
                     PEIIPVDLKEQALLRLEVGAERPTSYWVYALVSPFPTLEQGVSSDELRNTWNGNPAGP
                     FAGKPILLDESTLAVLTALWGKPADGAVQVMAAVELTGYAWDRSPWWAIIPFESLGPR
                     WKVLEVDGISPLRKEFDPASYGLAVPVSLNGDAALAEAILSMYAAGSSAALVPTGNRD
                     PNKLTVLAMTGVTALVRATAYTMEQRGIKYPAKDIGDWLRDADITHVSNEVPFAKNCP
                     FPDPVQQGMRFCSDPRYIALMEDVGTDIVELTGDHFQDWGEEAMYYTLDLYKERGWLY
                     YGGGEDLQEGRQAVTIEHNGNRLAFIGCNGKGGSFAQAARNHPGAVSCNFEYMEAEIA
                     RLRAEGYLPVATFQHFEYYTYRAQPDQVRDFRRLAGAGAVIVSGSQAHQPQAFEFEAG
                     ALIHYGLGNLFFDQLDVSQATRQAFIDRHVFYDGRYIGTELLTMLFVDYARARPMTSG
                     EREELLRAVFNASGW"
     gene            2846..3400
                     /gene="dcd"
                     /locus_tag="AC16V7_15750"
     CDS             2846..3400
                     /gene="dcd"
                     /locus_tag="AC16V7_15750"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HIR6258626.1"
                     /translation="MGLKPDHWIRKMAREQRMIEPFADSQVRDGVISFGVSSYGYDIR
                     VADEFKIFTNVFSAVVDPKQFDPRSMVDYKGEVCIIPPNSFALAKTIEYFRIPRKVLT
                     VCLGKSTYARCGIIVNVTPFEPEWEGYVTLEISNTTPLPAKIYANEGIAQVLFFEADD
                     MCEISYADKKGKYQGQQSIVLPKL"
BASE COUNT          763 a         1048 c         1048 g          704 t
ORIGIN      
        1 ctcaccagcc tgagctgcaa cctgaacggc tcgtttgagc acgaagaacc agcactggcc
       61 tctcctaccc cggtgcaggc agaactcgcc acaaatacgc ctgaagccag ccctacgacc
      121 gccaagacac aagcgcccgc gcaacctaca cagacgcgcg agcccaccac attgccccct
      181 acaaataccc caacccaagc ggttgtccag attgggccgt gcgagggtga agtatgcctg
      241 gtgcccttcc agttcccctt tagccggcca atttccccac ccggtcgaga ttttgtcgac
      301 atctcctatc gctttggcag cagcgaccac ggcaagcgtg acccccacca cggcgtggaa
      361 ttcctgaact cgcatggcac gcctgtattt gcggctgccg acggcgaggt gctggtggca
      421 ggcgacgaca ccaaggtaaa actcggattg tatccaaata tgtacggcaa cctggttgtg
      481 ctgcagcacg aagtgccggg tttcgagccg cccatcttta ccctgtatgc gcatttgtcg
      541 caggtggatg tacaggttgg agacagggtg gaggccgggg acaaattagg ccaggtaggc
      601 gccagcgggg cagctattgg cagccatctg catttcgaag tgcgctatgg cgagaacacg
      661 tatgatgcct cacgcaaccc ggagctgtgg ctgaaaccct tgatggatgg tagcggacag
      721 cctatggggg cgctggcggg gcgcatcctg gacgggaagg accagccgct gaaagtcgcc
      781 aacatcgtgg tggaatccct ctcggggagc acttattatc tcaatacgta ttcagaaacc
      841 aagctggtag ggctcgaccc gtggcaggag agtttcgcca tcggcgatct ccccgcaggc
      901 gagtatcaga tcagctttgt ccaaggcggc atacagcagc gcaccatcga ggtgcagcct
      961 ggggtgttga cgctggtgac gttccgcatt ggagggcagt aaggggtgaa gcatgaacca
     1021 tgagcaggcc ctaaaggtcg ctatgatctt tagggtctct gccttaagag ccagctttct
     1081 tgggttgtcc gtgatcttgg cctcctgcaa ggcagccgcg ccattggttc cgctactcca
     1141 aacagggctt tctcagccga cgatcacata cacacccttc cagccgctcc caccttcagc
     1201 tatgcccacc acaccgccac ccccaacggc tacagctgtc ccgaccgcgc ctgcaggcct
     1261 gaccctatgg gtagatccga gcttgccgga cgcactgcgc tcagccgtaa aaccaccacc
     1321 cgagattatc ccggttgatc tgaaagagca ggcgctccta cgcctggaag tgggcgcaga
     1381 gaggccgacc agctactggg tttacgcctt ggtatcgccc tttcccaccc tcgagcaagg
     1441 tgtatcatcc gatgagctac gcaatacctg gaatggaaac cctgctggac cgttcgctgg
     1501 caaaccgatc ttgttagacg agagcaccct ggcggttttg acagcgctgt ggggcaaacc
     1561 agccgatggt gcggtccagg tgatggctgc cgtggagttg acgggctacg cctgggatcg
     1621 cagcccgtgg tgggcgatca tcccatttga gagccttggg ccgcgctgga aagtgctgga
     1681 ggtagatggc atctcccctc tgcgaaaaga gtttgacccg gcgagttatg ggctggctgt
     1741 gcccgtttcg ctgaacggcg atgcagctct agcggaggcg attctctcga tgtacgcagc
     1801 gggatcaagc gctgcgctgg tcccgacagg caaccgcgat ccaaacaagc tgaccgtgct
     1861 ggccatgacg ggcgtgactg ccctggtgcg cgccacggca tataccatgg agcaacgcgg
     1921 catcaagtat cccgccaaag atattggtga ctggctgcgg gacgcggaca tcacccatgt
     1981 cagcaacgaa gtgcctttcg ccaaaaattg ccccttccca gacccggtgc agcagggcat
     2041 gcgtttttgc agcgacccgc gttatattgc cctgatggaa gacgtgggca ccgacatcgt
     2101 ggagctgacc ggcgaccact ttcaagattg gggcgaagaa gcgatgtact ataccctcga
     2161 tctatacaag gagcggggct ggctgtacta tggtggcggg gaagacctgc aggaggggcg
     2221 ccaggcagtg accatcgagc acaacggcaa tcggctggcc tttatcggct gcaacggcaa
     2281 agggggcagc tttgcccagg cggcacggaa tcaccccggg gcggtgtcct gcaatttcga
     2341 atacatggag gcggagatcg cccgcctgcg agcggagggc tacctgcccg tcgccacctt
     2401 ccagcacttt gagtattaca cctaccgtgc ccagcccgac caggtacgcg acttccgccg
     2461 cctggcgggt gccggggctg tcattgtcag cggcagccag gcgcaccagc cgcaagcgtt
     2521 tgagttcgag gccggggcgc tgatccatta cggcttgggc aacctgtttt tcgaccagct
     2581 cgatgtcagc caggccactc gccaggcgtt tatcgaccgc cacgtgttct atgacgggcg
     2641 ctatatcggc acagaactac tgaccatgct gtttgtggac tacgcccgtg cccgcccgat
     2701 gacctctggg gagcgggaag agctgctgag agccgtgttt aatgcaagcg gatggtaatc
     2761 aaataaatat gcaattcatg ggtttagtca tgcttaacag acaacccatt gagctgataa
     2821 aacttattgt aaaaaggaga tatttatggg actaaaacca gatcactgga tacgcaagat
     2881 ggcgcgtgag cagcgcatga tcgagccgtt cgccgacagc caggtgcgcg atggggtgat
     2941 ttccttcggc gtctcgtcct acggttacga tatccgtgtg gctgacgagt ttaaaatctt
     3001 taccaacgtg ttctcagcgg tggtcgatcc caagcagttc gacccgcgct cgatggtgga
     3061 ctacaagggc gaggtgtgca tcatccctcc caactcattt gcgctggcta aaaccatcga
     3121 gtatttccgc atcccgagaa aagtcttaac cgtgtgtctt ggtaaaagca cctacgcccg
     3181 ctgcgggatt atcgtcaacg tgacaccttt cgagcccgag tgggagggct acgtcacgct
     3241 ggagatctcc aacaccaccc cgctgcctgc caagatctac gccaacgaag gtatcgccca
     3301 ggtgctgttt ttcgaggccg acgatatgtg cgaaatctct tatgccgata agaaaggcaa
     3361 gtaccagggg cagcaatcca tcgtcttgcc gaagttataa tatatagaat gggattcgcc
     3421 aatcgagtgt tgaacctcca ggccgagggg gcatacgctg tgctggcccg cgcccaggag
     3481 ctggaggcgc aggggcgcga aatcctgcac ctggagatcg gccagcccga tttccagacc
     3541 ttcccgcata tcagcgagcg cgg
//
LOCUS       DBNHOH010000872         4288 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1018128, whole genome shotgun sequence.
ACCESSION   DBNHOH010000872 DBNHOH010000000
VERSION     DBNHOH010000872.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1018128"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /gene="mltG"
                     /locus_tag="AC16V7_15755"
     CDS             complement(<1..882)
                     /gene="mltG"
                     /locus_tag="AC16V7_15755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR6258627.1"
                     /translation="MKRRSPLLAGLSLLFFFFLCVLILVILNITLVIPRRAAQTFGLP
                     TDKLSYTQQLYYATLLTLQEESLTRPHNPLGEARLFEVGFGEPTASVIQRLQAEGMIT
                     SVEAFRNFLLYSGLDTTLQAGDYNLSPAMNAIEIAHALQDATPTHVTIRIFAGWRLEE
                     IAAALPTSGLDISPEEFLTSARIPRSGYVFSPLLPPNATLEGFFFPDTYQLSRTLTVE
                     GFIRTMLDNFQGHITPEIEQGYSNQGLNIYDAVILASIIEREAVVDEEMPQIASVFLN
                     RLAVGMKLDSDPTAQYAL"
     gene            complement(895..1314)
                     /gene="ruvX"
                     /locus_tag="AC16V7_15760"
     CDS             complement(895..1314)
                     /gene="ruvX"
                     /locus_tag="AC16V7_15760"
                     /inference="COORDINATES: protein motif:HMM:NF015607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR6258628.1"
                     /translation="MRILAVDPGEKNLGIAISDPSGTIANPLTVLRHVSRPIDAAAIA
                     DLASEHGAGLIVVGQAIDEEGNPTPQGRRSARLAAAIRTQTRVPVTLWDESGSTQAAR
                     LARLELGVSRAKRSGHLDELAATYILQTFLDARKHRD"
     gene            complement(1295..2818)
                     /locus_tag="AC16V7_15765"
     CDS             complement(1295..2818)
                     /locus_tag="AC16V7_15765"
                     /inference="COORDINATES: protein motif:HMM:NF016736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HIR6258629.1"
                     /translation="MKTQILRLDPHDDVISTRDKMNWGQTGRILLLWPEHGRVLRRRV
                     DLVLLHRHSNSLGAQLALVTNDPLVRFHAHYLAIPVFKNLRQAQSAHWRVDRQHRVRQ
                     ATKFVARPLLTSEALEELRQGAHPPTPAWLSKPFARLGFFGLGVIALLALAAVLLPGA
                     EVSLTPETQSQQLTISVRADPDQQDINYSGIVPARPVSVIVEGRDSIEASGVDQVADK
                     PSTGNVVFTNLSDQPVKIPVGLVVRAVSDPSVRFATIEAVEVAAGPGITTTLAVRAVT
                     PGKGSNLPAGSLNAIEGDFGLILAATNPAPTAGGTERTLPVPTIQDRKRLYEKLEAAL
                     RISALNEVQSQLAPDDLLFTPTLTITKVLDETYTPSSDQPSEQLQLNLRLEFQILSVF
                     RSDLENLAIVLLDTNLPNGFVPVPGDLILRQDSPPIMNEISVASWRVTAVRDLKADIQ
                     PAQVTNLLLGIPPDQAVARLSAALPMEAPPVIRLTPHWWPRLPVLPFRIVINENLSR"
     gene            complement(2829..>4288)
                     /locus_tag="AC16V7_15770"
     CDS             complement(2829..>4288)
                     /locus_tag="AC16V7_15770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR6258630.1"
                     /translation="IKIMVALRAMFISLMIGVLIVLALRSLFKDTTKAAVLTTLALAL
                     FYSYGHVYNSLERSALFGISLGRHRILAPMWMGILLASMIWVYRTKNPFSGLNRLLNL
                     VTVVALALPLAQIGLFGLRSMSATTEAPGQINIAGLHLPSDRPAPDVYYIILDAYARD
                     DTLIEEFELDNTPFLSQLEAMGFYVARCSQSNYVQTQLSLSSSLNMDYLQSLDPGYSP
                     GRKTRVGIEELIRHSAVRQAFKNLGYTTIAFETGFKGTQWEDADIYLSPSNDLFHKLQ
                     IAGGPNGFEVLLLRTSAGLLLADGAVVLPKIFQPDFDNPRKIHRDLILFDLEQLHVLP
                     TIPGPKFVFAHLVTPHPPYVFGPNGEFTDFDKEYKPGYRDQVIYLNKRLLELIPTMIA
                     NSPVPPIIILQGDHGSIGSPPNKRTTILNAYYLPDGGNKSLYESISPVNTFRIIFNSY
                     FEGQYELLEDVSYYSVYTDPFDFTVIPNKRADCPS"
BASE COUNT         1034 a         1020 c         1212 g         1022 t
ORIGIN      
        1 cagggcgtat tgggcagtgg gatcagaatc tagcttcata ccaacagcca aacgattgag
       61 aaatacagac gcgatctgag gcatctcttc atccaccact gcttcgcgct cgatgatgga
      121 ggccagtata acagcatcgt agatgttcaa gccctggttg ctataacctt gctcaatctc
      181 cggggtaata tgtccctgga aattatccag cattgtcctg ataaaaccct caactgtcag
      241 cgttcggctc aactggtagg tatctgggaa gaaaaacccc tccagggttg cattaggtgg
      301 gagcagagga gaaaagacat aaccactacg tgggatgcgt gcagaggtca ggaactcttc
      361 cggggagata tccaatcccg aagttggaag ggctgctgca atttcttcta agcgccagcc
      421 ggcgaaaatt cttatggtaa catgggtggg cgtagcatcc tggagcgcat gggcaatctc
      481 tatcgcattc atggctgggc tcaggttgta atcccctgcc tgcaaggttg tatccaagcc
      541 gctatacagc agaaaattgc ggaatgcctc gacgctggta atcatccctt ctgcctgcaa
      601 acgttgaata accgaggcgg ttggctcgcc aaaaccgact tcaaaaaggc gcgcttctcc
      661 aagcggattg tgcgggcggg tgagtgattc ttcttgcagg gtgagcaagg tagcgtagta
      721 tagctgctga gtgtagctaa gcttgtcggt aggcagccca aatgtctgcg ccgcgcggcg
      781 tggaatcacc aaggtgatgt ttaggatgac taatatcagc acacatagga agaaaaagaa
      841 aagcaggctg agaccagcta ggagtggtga gcgtcgtttc attgatcgaa ggccctagtc
      901 tctgtgtttt ctggcatcga gaaatgtttg caggatgtag gtggcagcta actcgtcgag
      961 gtgcccgctt cgtttggcac gtgatacacc taactccaat cgcgcaagac gggcggcttg
     1021 tgtgctaccg ctttcatccc acagggtcac aggcactcga gtttgcgtgc ggatggcagc
     1081 cgccaggcgg gcagaacggc gtccttgcgg tgtgggatta ccctcctcat caattgcctg
     1141 gccgaccacg atcaagcccg caccatgctc gctagccagg tcagcgatag cggccgcgtc
     1201 gatgggtcgc gatacatggc gcaggacggt taacgggttg gcgatggttc cactgggatc
     1261 actgatggcg attcccagat tcttttctcc ggggtcaacg gctaagattc tcattgatta
     1321 caatccgaaa aggtaggacc ggtaggcgcg gccaccagtg tggagtcagt cggatgactg
     1381 gcggagcctc cataggcaag gcggcgctca gccgtgccac cgcttgatct ggggggatac
     1441 ctaggagaag gttagtaact tgcgctggct ggatgtctgc cttgagatct cgcacggcag
     1501 tgaccctcca actagcgaca ctaatctcat tcatgatagg tggtgagtct tgtcggagga
     1561 tgagatctcc aggcacaggg acaaaaccat ttggcaagtt tgtatccaag agcacgatcg
     1621 ccaagttttc caaatcggaa cgaaacacgc tgagtatctg gaattccagg cgcagattga
     1681 gttgaagctg ttcagagggc tggtcgctgc ttggtgtata agtctcgtcc agcactttgg
     1741 taatggttag tgttggtgta aagagcaggt cgtcgggagc aagttgcgat tggacttcat
     1801 tcaaggcaga tattcttaga gcagcctcca gtttttcgta tagccgcttc ctgtcttgga
     1861 tggttggcac gggaagagtg cgctcggtgc caccagcggt tggagcagga tttgttgcag
     1921 ctaagatcaa cccgaagtcc ccttcgatgg cgttcaggct cccagctggc aagttacttc
     1981 ctttgccagg ggtgacagct cgcactgcca gcgtggttgt aatacccgga ccggcggcga
     2041 cctcgacggc ttcaatggta gcaaaacgta ctgatggatc agacactgcg cgcactacca
     2101 atccaacagg gattttcacg ggctggtcgc tcaggtttgt gaaaactaca ttacccgttg
     2161 atggtttatc ggcaacttga tcaaccccac tggcttcgat gctatcgcgc ccctcaacga
     2221 tcactgaaac cggtcgggca ggtactatgc ctgaatagtt gatatcctgc tggtctgggt
     2281 cggcgcgcac ggaaatggtg agttgctggc tctgggtctc gggagtgagg gacacctccg
     2341 ccccgggtag gagtacagct gccaaagcca gcaaggcgat tactcccaaa ccgaagaatc
     2401 ccaatcttgc gaatggtttc gatagccagg caggagtggg gggatgtgct ccctggcgca
     2461 gttcctccaa cgcttcgctc gtcagaagcg ggcgagcgac gaattttgtc gcttggcgca
     2521 cccggtgctg tctatctacc ctccaatgag cgctctgcgc ctggcgtaaa ttcttgaata
     2581 caggtatagc aaggtaatgg gcgtgaaagc gtaccagcgg gtcattggtg accaacgcca
     2641 gttgtgctcc cagggaattg ctgtgccgat gcaggagcac caggtctaca cggcggcgca
     2701 aaacgcgacc gtgttcaggc cataacaaca atatacgccc ggtctgtccc cagttcattt
     2761 tgtctcgggt agatattaca tcatcatgtg ggtccaagcg cagaatttgt gttttcattc
     2821 aaccgctctc aagatggaca atccgcacgc ttgtttggga tgacggtaaa atcaaatggg
     2881 tcagtgtaga cagaatagta gctcacatct tccagcagtt cgtattgacc ttcaaaatat
     2941 gaattgaata ttatacgaaa agtgtttact ggggagatcg attcgtagag ggatttattt
     3001 cctccgtccg gaaggtaata ggcattaaga atggttgtac gtttattggg agggctgcca
     3061 attgagccgt gatccccctg aaggatgatg attggtggga cgggtgagtt ggcgatcata
     3121 gttgggatga gctcaagtaa gcgtttgttc aagtagatca cctgatcgcg gtatccgggt
     3181 ttgtactctt tatcaaaatc ggtaaactct ccatttggac cgaatacata gggagggtgc
     3241 ggggtcacca ggtgtgcgaa gacgaacttg ggacccggga tagttggtag gacatgcaat
     3301 tgttcgagat caaagaggat caaatcccgg tggatttttc gaggattgtc aaaatctggc
     3361 tggaatatct ttggcaagac gacagcccca tcagccagaa gcaggcctgc cgacgtcctt
     3421 agcagcagca cctcaaatcc attcggcccg cctgcgattt gcagcttgtg gaataagtca
     3481 ttggagggag ataggtaaat gtcggcatct tcccattgtg tgcccttaaa tccggtctca
     3541 aacgcgatgg ttgtatatcc tagattttta aaagcctgcc gcactgcgct gtgtcggatt
     3601 aactcttcaa tgcccacccg tgtcttacgt ccaggagaat agcctggatc taaagattgc
     3661 aggtagtcca tatttaggga tgacgagaga gatagctgag tttgtacata attgctctgg
     3721 ctgcagcggg cgacgtagaa acccattgcc tccaattggc tcaggaatgg ggtgttatcc
     3781 agttcgaact cttctatcaa ggtgtcgtcg cgagcgtagg catccaggat gatgtagtag
     3841 acatcgggcg cgggtcgatc ggaggggaga tgtaatcctg cgatattgat ctgacctggc
     3901 gcttccgtag ttgcggacat gcttcttaac ccaaataatc caatttgggc caggggtaga
     3961 gccaaagcga ccacagtaac caagttgagc agacgattca aaccagagaa tggatttttt
     4021 gtccggtaaa cccagatcat ggatgccagc aatattccca tccacatggg tgccaaaatg
     4081 cgatgtcttc ctagactgat accaaaaaga gctgatcgtt ccagtgagtt gtaaacgtgc
     4141 ccataagaat agaaaagagc taatgccaaa gttgtcaata cagcagcttt ggttgtatct
     4201 ttgaacagac tcctgagggc gagaactatt aggacgccga tcatcaggga aatgaacatg
     4261 gcgcgcaagg cgaccatgat cttgatct
//
LOCUS       DBNHOH010000873         4292 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_761715, whole genome shotgun sequence.
ACCESSION   DBNHOH010000873 DBNHOH010000000
VERSION     DBNHOH010000873.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_761715"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="AC16V7_15775"
     CDS             <1..283
                     /locus_tag="AC16V7_15775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258631.1"
                     /translation="RILAQAQEEANRTITIAREKLDQLVERDAIVQAAHAKAGEVLAM
                     AQVERESTRKEADEYALDTLRRLEMELERFLNQVRNGISVLHSEKQEPE"
     gene            complement(335..1138)
                     /locus_tag="AC16V7_15780"
     CDS             complement(335..1138)
                     /locus_tag="AC16V7_15780"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6258632.1"
                     /translation="MTRSSPVLLVTGASSGIGEATAKLFAAHGYRVVLAARRRERLEA
                     LGEEIRKMGGDALPVVADLTDLRQIEQLVDSSLSDYGRIDVLFNNAGMGRMNWLENLD
                     PVADIEALFRVNLLGVVQTTRAVLPHMIMRKSGHIINMASMAGYLATPTYSIYAASKF
                     AVRGFSDALRREVGVYGIRVSGIYPSGVETEFARHAGIKRKTGISTPARLRLSAEQVA
                     ISVLDVVHRPRRAVLIPAPMRMVAWFGFFFPGFVDWIIEKRFTRPERGL"
     gene            1330..1707
                     /locus_tag="AC16V7_15785"
     CDS             1330..1707
                     /locus_tag="AC16V7_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6258633.1"
                     /translation="MLDNPKQVVYIEDEQEMIELVRLILSRKGYEVLGASGGREGLDL
                     VRQILPDLVLLDLMMPDMDGWEVYQQMRAEEATRDIPVIVITAKAQSIDKVLGLHIAK
                     VDAYISKPFSPHDLVESVERVLS"
     gene            1791..2168
                     /locus_tag="AC16V7_15790"
     CDS             1791..2168
                     /locus_tag="AC16V7_15790"
                     /inference="COORDINATES: protein motif:HMM:NF014678.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HIR6258634.1"
                     /translation="MKNIVIQNTSRPTSKPVRAKYCSSFMCRLRGFTFRRNLTSDEAL
                     LLVQLKDSRLDAAIHMLAVWTDLAVIWINNQGVVVDLCTARAWRPFYIPKQPARYILE
                     MAPSRLSEFAIGDKVTLEDETED"
     gene            2152..3561
                     /locus_tag="AC16V7_15795"
     CDS             2152..3561
                     /locus_tag="AC16V7_15795"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR6258635.1"
                     /translation="MKLRINLFLLMCLLFAVLPVRAQEEQPQGPVYTVQAGDTLGGIA
                     RRFGLTIDELALANGISNPNLLSEGTQLVIPGLDGIQGFLTTVTVPFGENLSSISRRY
                     QIDIALLARLNHLTAPSELYAGADLVIPQEETGATNLTRMALAEGQSLLELSILAGTN
                     PWSVLRGNQIMNSWGALPGDVYLLPGDGEAGPGGLPGAIQDVEITPLPLRQGKAGVIK
                     IAAPQGLELEGSFMGYPLNFFSDTEGGYVALFGIHAMTEPAFYPFHLQGKLSDGRPFT
                     FTQRVGVRLEDYPFDQPLTVDPATIDPAITRPEDAQWSALAATATPERLWDGLFRMPS
                     PLSQDYCVETNECWTSRFGNRRSYNGSPYAYFHTGLDVAGGTGTKIFAPAPGVVVFAG
                     PLTVRGNATMIDHGWGVYTAYLHQSEIMVEVGDRVESGDEIGLVGGTGRVEGPHLHWE
                     VWVNGIQVDPLDWLIQPFP"
     gene            complement(3611..4120)
                     /locus_tag="AC16V7_15800"
     CDS             complement(3611..4120)
                     /locus_tag="AC16V7_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HIR6258636.1"
                     /translation="MTLRIGILTVSDRSSRGERADTSGPAIQAVVERQGWVVSKTGII
                     PDHLEAIQEMLIDWVDSEEVDVILTTGGTGFSRRDVTPEATLAIIERAAPGISEAMRS
                     ASLQVTPHAMLSRAMAGIRGRTLVINLPGSPKGAVENLQVVLPVLSHAVQLLRDEPDA
                     EAGHLHHDG"
     gene            complement(4117..4278)
                     /locus_tag="AC16V7_15805"
     CDS             complement(4117..4278)
                     /locus_tag="AC16V7_15805"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HIR6258637.1"
                     /translation="MGDSLMNEVESRLKEKGCIRAYLLIAHDNGSVVDFYKRRDWETM
                     NLTIMAKDL"
BASE COUNT         1054 a         1178 c         1085 g          975 t
ORIGIN      
        1 ccgcatcttg gcgcaggcgc aagaagaggc caaccgcaca atcacaattg ctcgcgagaa
       61 gctggatcaa ctggttgaac gcgacgccat agtgcaagct gcccatgcca aagccggcga
      121 agttttagcc atggctcagg tagaacgcga atccacacgc aaagaagcgg atgagtacgc
      181 ccttgatacc ttacgccgtt tggaaatgga attggagcgc ttccttaacc aggtgcgcaa
      241 cgggatctct gtactacaca gtgaaaaaca agaaccagag taaaagaaac cacataaaaa
      301 aagattagcg ggcaccaaaa gcccgctaat ttgtttaaag gcctctctca gggcgggtaa
      361 aacgcttttc aatgatccaa tccacaaatc ctgggaaaaa gaaaccaaac catgcgacca
      421 ttctcatcgg tgcagggatc aacacggctc gtcgcggcct gtgtactacg tccaacacgc
      481 taattgccac ctgctcagcg ctcaaccgca gtctcgcagg cgtgctgatg ccggtcttgc
      541 gctttatccc tgcatgccgg gcaaactccg tttccacacc acttgggtag atccccgaaa
      601 cgcgtatgcc atatacgccc acttcacgcc ttaaagcatc actgaagccg cgcacagcga
      661 atttgctggc ggcgtaaatc gagtaggtcg gggtagccaa ataaccagcc atagaagcca
      721 tgttaatgat atgcccgctc ttcctcataa tcatgtgggg cagcaccgct cgtgttgtct
      781 gcaccactcc aagaaggttg accctaaata acgcttcaat atcggcaacc gggtcgagat
      841 tttcaagcca gttcatgcga cccatgccag cattattgaa aagcacatcg atccttccat
      901 aatctgaaag agaactatct acaagctgtt caatttgccg taaatcagtc agatcagcaa
      961 caacaggtag cgcatcccca cccattttcc gtatttcttc ccccagcgcc tctagacgct
     1021 cgcgccgccg cgcagccagt acaacacggt agccatgtgc ggcaaaaagc ttcgcagtgg
     1081 cctctccaat accggaggat gcgccagtaa cgagcagaac cggggaactt cgtgtcataa
     1141 tgcacctctt gcaaatgatt atccataagt ttgctttaga gtcaacccga gctggacagg
     1201 tccccaaagg aaaggggatt ggactttcgg ttttttgatt actctgcaga ggtagatatg
     1261 ccatattcca atttttgatg gatatggtaa acttatgcgt aacaagccaa gcctggatcg
     1321 gaagggtcta tgctagataa ccccaagcaa gtagtttaca ttgaagatga gcaagagatg
     1381 atcgagcttg ttcgcttgat actcagccga aaaggctacg aagtgctagg agccagcggt
     1441 gggcgcgagg ggctagatct agttcggcaa atactgcctg acttagtcct cctggatttg
     1501 atgatgccgg atatggatgg atgggaagtc taccaacaaa tgcgggcaga agaagccacg
     1561 cgggacattc cggtgatcgt catcactgct aaggctcaga gcattgataa agttttgggg
     1621 ttgcacatcg ctaaagtcga tgcctacatc tcgaaaccat tcagcccgca tgacctggta
     1681 gaaagtgtgg agagagtctt gtcctgacag caacccatca catccattca acttataata
     1741 gactttgggg tctgcaggcg gcattaccgt cccgggcccc aagttagctt atgaagaaca
     1801 ttgtcattca aaataccagc cgtccaacaa gcaagcctgt tcgggcaaag tattgctcat
     1861 ccttcatgtg ccgattgcgc ggcttcacat ttcgacgaaa tttaacctcc gatgaggctt
     1921 tgctgcttgt ccagttaaaa gatagccgcc tggacgcagc tatccatatg ctggctgtct
     1981 ggacggacct ggcagtgata tggatcaaca accaaggtgt agtggtggat ttatgcaccg
     2041 cgcgggcgtg gcgccctttc tatataccca aacagccagc gcgctatatt cttgagatgg
     2101 caccctcgcg cctttcagag ttcgctatcg gagacaaagt tacacttgaa gatgaaactg
     2161 aggattaacc tgtttttact catgtgtctc ctctttgcgg tcctcccggt gcgcgcccag
     2221 gaggaacaac cccaagggcc agtttatacc gtacaggcag gcgataccct ggggggaatt
     2281 gcccggcgat tcggcctgac aatagatgaa ctggcattag cgaatggcat ctccaacccg
     2341 aatttactct cggaaggcac ccagctggtt atcccgggtt tagacgggat tcaaggattt
     2401 ttaaccacgg tcacggtccc atttggcgaa aacctgagca gtatcagccg tcgctatcag
     2461 atagatattg ccctgctggc acgtttaaac catctcaccg caccgtcgga gctatatgcg
     2521 ggagcagacc tggtgatccc ccaagaagag acaggtgcaa ccaacctcac acgaatggct
     2581 ctggctgaag gtcaatcttt gctggagctc tctatattag ccggaaccaa cccctggagc
     2641 gttctgagag gtaaccaaat aatgaattcc tggggggcgc tacctggtga cgtctatcta
     2701 ctacctgggg atggagaggc tggtcctggc ggactgcctg gagcaatcca ggatgtagag
     2761 ataacgccac tccctttgcg ccaaggtaaa gccggggtga ttaagatcgc tgctccgcaa
     2821 ggattggaac tcgaggggtc cttcatggga tacccgctga acttcttctc cgacactgag
     2881 gggggatatg tagccttgtt cggcattcat gccatgacag aaccggcctt ctatcccttc
     2941 cacttgcagg gcaagctttc tgatggcagg ccttttacct tcactcagag ggttggggtt
     3001 agattggagg attacccctt tgatcagcct ttgaccgtcg acccggctac gattgacccg
     3061 gcaattactc gtcccgaaga cgcgcagtgg tcagcactag cggctaccgc cacgccagaa
     3121 cgcctctggg atggactatt ccggatgcct tcgccacttt cccaggatta ttgcgtagaa
     3181 accaatgagt gctggacatc tcgctttggc aatcgtcgct cctataatgg aagcccatat
     3241 gcctatttcc acaccggatt agatgttgcc gggggcaccg gcaccaagat ctttgccccc
     3301 gcgcccgggg ttgtcgtatt cgctggtcca ctcactgtgc gcggcaacgc caccatgatc
     3361 gaccatgggt ggggtgtgta cacagcctac ctgcaccaat cagaaattat ggttgaggtt
     3421 ggcgaccggg ttgagagcgg ggatgaaatt ggcctggtag gcggcacggg tcgcgtagaa
     3481 ggaccccatc ttcactggga agtttgggtg aacgggattc aggtcgatcc ccttgactgg
     3541 cttatacaac catttccata gagattaagc gtatctgctg gcttgcgcca aatacaaatg
     3601 tcacgcttgc tcacccgtca tgatgcaggt gtcctgcctc tgcatctggt tcatcgcgca
     3661 ggagctgcac ggcatgtgac aaaacaggca gtacgacctg caaattctcc actgcgccct
     3721 tagggcttcc aggcagatta atcaccaacg ttcgccctcg gatgcctgcc atagcccgag
     3781 agagcatggc atgcggcgta acctgtaagc tggcggagcg catggcttca gagatccccg
     3841 gggctgcccg ttcaatgatc gcaagcgtcg cttcgggtgt cacatcacgc cgactgaagc
     3901 ctgtccctcc ggtggtcaaa atgacgtcca cttcctccga atcgacccaa tcgatcagca
     3961 tctcctggat ggcttctaag tggtcgggaa tgatgccggt cttcgacacc acccacccct
     4021 gtcgctcaac gactgcctgt attgctggtc cagaagtatc agcgcgctct cctcgggagg
     4081 agcgatcaga aacggtcaag atgccgatgc gtaaggtcat aaatctttcg ccataatcgt
     4141 gagattcatc gtctcccagt cacgacgctt gtaaaaatcc acaacactcc cgttatcgtg
     4201 agcaatcagc aaatatgccc ggatgcagcc tttttctttc agcctgcttt ctacttcatt
     4261 catcaaagaa tcccccaagc ctctaccacg gt
//
LOCUS       DBNHOH010000874         4228 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1402350, whole genome shotgun sequence.
ACCESSION   DBNHOH010000874 DBNHOH010000000
VERSION     DBNHOH010000874.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1402350"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..3943
                     /locus_tag="AC16V7_15810"
     CDS             14..3943
                     /locus_tag="AC16V7_15810"
                     /inference="COORDINATES: protein motif:HMM:NF012307.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HIR6258638.1"
                     /translation="MIHDSADKQYQILELTLDPAAKGRVQGVRLTPQEGQTHEPFQLL
                     NVYLPHDDLGKIECFKAMVNLPFDDRTAIIGDWNFIESPKDATNPNTNLLITGAAKDA
                     WEILVDRYQLREIRQPTHTHYFIASDPALTRTSRIDRAYISTDQAADDATVPTLHIPH
                     TPHSVLKAHNTNNRNKSLTDHTPLLLSFHPHGPTQQRKPRIFHPWMADVNSITEYIGR
                     NINSTLHSSHDPFHALSRFTELAHTGAKRYWRSIKDKKVGFTNDLHELTIATKLYRLC
                     SAVHPDADQITALVSRYPSINKHVNPTTNPHGVSVYDLSALARRIDELTQATTDPTNL
                     TNLPPSTTPAAQQHNRDLFDEVQEASWDFKARLRAVRPSMEDNPTDDPKEMANVILPF
                     WKEVWSKRSDPPSRTQTRNYLADYPKRVPETLLPKMPSQDDIVDGILKTTNTASGPDG
                     IPFALIRRFAHELAPILLRCIEELGAGRSPPPAFNAGHLHLFPKNDSQLVQDLRPITV
                     GNAFNRIVAKLLVFAITPALQQLLSPSQKGFVPGRDGADSIETITEQYYSALLRKHQQ
                     FILFLDTRKAFDSLDHRFIQETLRHVGMPTWLCNAVVGLMSDVKITPMIFLCRAKIAI
                     ERGVKQGCPLSPLLFVLAYDVLLHRLSSNKDLRHYAFADDLAVESQNIGHIRSALRTI
                     TRYSRISGLGINIHKTSILSTMPVTDWDRAVLGTDYRNIKFVDAATYLGVPIGRFLTL
                     EDVFAKPLDKFLERLRKLRRLLKRYSLHKRILVANIYLLPLFLYLARFYTIPYSIIVR
                     VRRALHTAIVAFHGTAFSYPHLISKAFGPHTPLKDLWATNMALLATGAIDASNHGRMT
                     SDISAAPQVTSPHWSSMLIKENRQSAVFVYLEDYNDRADDLSIRIDHLSERLPRQRAQ
                     IYEELVRNGYWRERTSQQASYKGSLYHKAHKHIDAPIHPSIHDPASHLESLAPLRRVA
                     GSNPWNTFLKLTMNALATDCRRHKAGIEVPQRGPPANPFPCYFCGQDRDHVTHMYSEC
                     SVTGEALHRVSTAAGAGFTHTLTDSLTLALPRDNTHILYRIAFNWAVWRTRQDFLITR
                     THPPPPEQTVNRITTMALNHALAAGKTASKSRAYNPRVLSMARAPQADRLICFTDGSA
                     MPNPGPTGAGVHYHLPATGDEHSITIALGEGDNNLGEIVGLGIAISISTLYQDDTALI
                     FSDSLSSIYYLTAGWPHPADKRISRATRRTYRNIAPAKRPRLYWVRGHANIDGNEHAD
                     KYAKRGAAISKAGRHNYPYVRYSINTPPHIKLVVDDFLNYMYDSLPP"
BASE COUNT         1042 a         1404 c          954 g          828 t
ORIGIN      
        1 cggcacgttg atcatgatac atgactcggc cgataaacag taccagatcc tagagctaac
       61 actcgacccg gccgccaaag gccgcgttca gggggtacgc ctaacccccc aagagggaca
      121 aacccacgag cctttccagc tcttgaacgt gtacctcccc catgacgatt taggcaaaat
      181 tgagtgcttc aaagctatgg ttaatctccc cttcgatgat cgaactgcca tcatcgggga
      241 ctggaacttt attgagtccc ctaaggacgc taccaatccc aacaccaacc tcttgattac
      301 aggcgcagcc aaggatgctt gggagatcct ggtggaccgc taccagctac gcgagatccg
      361 ccagcccacc cacacgcact acttcatcgc cagcgacccc gcactcacgc gcacttctag
      421 aatagatagg gcgtacatct cgactgatca ggcagcagat gacgccactg tacccacact
      481 ccacatccct cacacccccc actccgtcct aaaagcgcac aacactaaca accgcaacaa
      541 atccctcaca gatcacactc ccctccttct cagctttcat ccacacggcc caacgcagca
      601 aaggaagcca aggatcttcc acccctggat ggccgacgtc aacagtatca ctgagtacat
      661 cgggcgcaat ataaacagca cactgcatag ctcgcacgat ccctttcatg ctctgtccag
      721 gtttaccgag cttgcgcaca ctggggccaa gcggtactgg agatctatca aagacaaaaa
      781 ggtaggcttc accaacgacc tccacgagct gacaatagct accaagctat acagactatg
      841 cagcgctgtg catcccgacg ccgaccagat cacagccctg gtttcacgct acccctccat
      901 caacaaacac gtcaatccca ccaccaaccc gcacggtgtc tccgtctacg atctcagcgc
      961 tcttgcacgc cgcatcgacg aactcacgca ggctacgacg gacccgacca acctcacaaa
     1021 cctccctcct tcaactaccc cagcagccca gcaacacaac agagacctct tcgacgaagt
     1081 tcaggaggct tcctgggact tcaaggcgcg gttgagggcg gtgcgcccaa gcatggagga
     1141 caatcctacg gacgatccca aggagatggc taatgtcatc ctccccttct ggaaagaggt
     1201 ctggagcaaa cgcagcgacc cgcccagccg cactcaaacc cgcaattacc tcgccgacta
     1261 ccccaaacgc gtgcccgaaa cgcttctccc gaagatgccc agtcaggacg acatcgtgga
     1321 cggcatcctc aagaccacca acactgcaag cggccctgac ggcatcccct tcgcgctgat
     1381 tcgccgcttc gcgcacgagc tagcccccat ccttctacgc tgcatagaag aactaggcgc
     1441 agggcgatcg ccgcccccgg ccttcaacgc aggtcacctt catctctttc ccaaaaatga
     1501 ttcgcagctt gttcaggact tgaggcctat cacggtgggg aacgccttca atcggattgt
     1561 ggccaaactc ctggttttcg ccattactcc agccctccaa cagctcctga gccccagcca
     1621 gaagggcttc gtgccggggc gggatggagc ggactctatc gagactatca cagaacagta
     1681 ttatagcgcg ctcttgcgca aacaccagca attcatactc tttctagaca ctaggaaggc
     1741 ttttgactca ctggaccacc ggttcattca agaaacgcta aggcatgtgg gtatgcccac
     1801 atggctctgt aatgccgtgg tgggcctcat gtctgatgtc aagatcaccc ccatgatctt
     1861 tctgtgcagg gcgaagattg ccatagagag aggggtcaag cagggctgcc ccctctcccc
     1921 gctcctcttc gtgctagcat acgatgttct ccttcatagg ctctccagca ataaggacct
     1981 ccgccactac gccttcgccg acgacttggc tgtcgaatcc cagaacatag gccacatccg
     2041 ctcagcactg cgcaccataa cacgatattc ccgcatttca ggtttaggga ttaacatcca
     2101 taaaacttcg atcctgtcga ccatgcccgt caccgattgg gacagggctg ttctcggcac
     2161 cgactacagg aatatcaaat ttgtggacgc tgcaacgtac cttggggtgc caataggcag
     2221 gttcctgacc ctggaagatg tcttcgccaa gccgctcgat aagttcctcg agcgcctccg
     2281 caagcttcgc cgtctcctca agaggtactc ccttcataaa cgcattctgg tggccaacat
     2341 ctacctcctc cccctcttcc tctacctagc taggttctac accataccct actctatcat
     2401 cgtcagagtg agacgggcac ttcatacagc cattgttgcc ttccacggca ccgcctttag
     2461 ctacccccat ctcatctcca aggcttttgg cccacacacc ccgctcaagg acctctgggc
     2521 cacgaatatg gcgctgctag ccaccggcgc gatcgacgct agcaaccacg gcaggatgac
     2581 atcagacatc tctgcggctc ctcaggtgac tagcccacac tggagctcca tgctgatcaa
     2641 agaaaaccgc caatccgcag tcttcgtcta cctcgaggac tacaacgaca gagccgacga
     2701 cctctcgatc cgaatcgacc acttatccga acgcctaccc cgccagagag cccagatcta
     2761 cgaggagcta gtgcggaatg ggtactggcg cgagcgcacc tctcagcaag caagctacaa
     2821 aggttctcta taccacaagg cgcacaagca cattgacgcg cccatacacc ccagcattca
     2881 tgaccctgcc agccatctag agagcttggc tccgctcaga agagtggctg gttccaaccc
     2941 atggaacacc ttcttgaagc ttaccatgaa tgccctggct acagactgcc gccgccacaa
     3001 agcgggtatc gaggttcctc aacgcgggcc gccagctaac ccgttcccct gctacttttg
     3061 tggccaagac agggaccacg ttacccacat gtactctgag tgtagcgtga caggcgaagc
     3121 acttcatcgg gtgagtacag ctgccggggc aggcttcacc cacaccctta cggactctct
     3181 cacgctcgca ctcccgcgcg acaacaccca tattctctac aggattgcct ttaactgggc
     3241 cgtctggcgc actcggcaag acttcttgat cacccgcacc cacccgccac cccccgaaca
     3301 aacagtgaat cggatcacta cgatggccct caaccatgct ctcgctgctg ggaagaccgc
     3361 tagcaagtcc cgtgcttata acccaagggt gctctccatg gccagggccc cacaggcaga
     3421 ccggctcatc tgctttacag acggctctgc gatgccaaac cccggcccga caggagccgg
     3481 agtccactac cacctccccg ccactggcga tgaacactct atcacgattg ctctaggtga
     3541 gggcgataac aatctaggcg agatagtagg cctaggcata gctattagca ttagcaccct
     3601 ttaccaggat gatacggcct tgatcttctc ggactcactg agctccattt actacctcac
     3661 tgcaggctgg ccacacccag ctgataaacg cataagtagg gcaacacgta gaacctaccg
     3721 taatatagcc cccgctaaac gccctcgcct ctactgggtc agaggccacg ccaatattga
     3781 tggcaatgag catgctgaca aatatgccaa acgaggtgca gccattagca aggctggtcg
     3841 gcacaattat ccgtatgtca gatacagtat taataccccc cctcacatca agttagttgt
     3901 agacgatttc cttaactaca tgtacgacag tttaccccct tagaatgggg gggaccccta
     3961 gctaggattg tacgccggac cacgccgctc tttatttccc aataaagatt tcgggtttta
     4021 aaaaaaaaaa aaacgaccga gagacgtagc cagccaccca ttgctggaac agtgggtgga
     4081 cgtgtcgttc gctccagagg tggacaggaa taggggaaag tccctcatag gttacatggt
     4141 agccttcaag ggggcaatca ccacttgggc gacaatgaag tccaagaggg tcctcacatc
     4201 caccaccgaa gccgagtgca ccgggcta
//
LOCUS       DBNHOH010000875         5757 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_55844, whole genome shotgun sequence.
ACCESSION   DBNHOH010000875 DBNHOH010000000
VERSION     DBNHOH010000875.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_55844"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1825
                     /locus_tag="AC16V7_15815"
     CDS             <1..1825
                     /locus_tag="AC16V7_15815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR6258639.1"
                     /translation="QADFVIAGEYHVPKVQQAHIEPHVVLTYWDEDDRLVIRTSTQVP
                     FHARRILAPVLGLPIKRIRVIKPRIGGGFGGKQEVLIEDVAAHLTIATGRPVVYEYTR
                     EEEFIAARSRHPMRVQMKTGVKKDGTITANEMRVLTDTGAYGCHALTVTGNTGHKSMA
                     LYVGDGPYRQKPNIRFYADVVYTNHPPAGAYRGYGVPQGYWPVERHMEKIARQLNLDP
                     LEFRLKNALRAGELHPFSTAWSEGREPRPETIETCGLEECARQGKASIGWDQKYGNLE
                     WHNPDPMRPYLKKGIGVALVMQGTAIPYLDMGGASIKMNDDGSFNLLVGATDLGTGSD
                     TVLAQMAAEVLGVPLEDMITYSSDTDFTPFDKGAYASSTTYISGAAVAKAADQVAQRI
                     KARALRMINEASSVETRQPLDVGDIRLQDRKAIAPDGRSVTLAEVALDALHHKDQEQI
                     MGVASYVSPVSPPPFAAQFAEVTVDTETGQVRVDKLVMAVDSGIIVNPVTASGQIEGG
                     MTQALGYAVCEEMRYDEKGSARERDFRDYHIFRADEMPELETIFVETFEPSHPFGVKA
                     AAEIPMDGVAPAVGNAVLDACGANIDDNPVIPEKVWRALRT"
     gene            1896..2363
                     /gene="bfr"
                     /locus_tag="AC16V7_15820"
     CDS             1896..2363
                     /gene="bfr"
                     /locus_tag="AC16V7_15820"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006365810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HIR6258640.1"
                     /translation="MKGNDNLIMVLNELLADELTAINQYMVHSEMCADWRYEKLHEAA
                     EARAIQEMKHAEKLIGRILFLEGKPNVSNLKRIHIGSDIKEQLVNDRMAEQEAVVAYN
                     KGIQLAVDVADNGSRELLESILKDEEDHLDWLEAQLEQIEQMGIQNYLVEQTG"
     gene            2374..3720
                     /locus_tag="AC16V7_15825"
     CDS             2374..3720
                     /locus_tag="AC16V7_15825"
                     /inference="COORDINATES: protein motif:HMM:NF013528.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA2 domain-containing protein"
                     /protein_id="HIR6258641.1"
                     /translation="MIERVYVIGHVNPDTDSIAAAVGYAWLLRERDGVDTVAARAGAI
                     NPQTTWVLKRLGLEPPLLLTDASPRFEVVTRRLDTARPDSLLREAWAITSRTRGLAPI
                     VGEDGTPFGLITGSSLFDFVAKLVGTHPRRQEMRISEILDLPCQEACDTKVPRFQAGT
                     RIRDVINRLLREEHNDFLIVDEKGLYLGVCRQRDAINPPRLRIVLVDHNEPGQALGSI
                     EEAELIEILDHHRLGNPSTHVPIKFTVDIVGSTSTLVSERMEEAGLSAPPALAGLMLA
                     GLLSDTLILTSPTTTERDRGAAERLARWAFVRNGPLAGENIHSFGEQVIGAGSGLSNR
                     DPEEVVSSDLKLYEAEDVSFAIAQVEITDLMQLDEHLTALKGALETLRQRRGVDFAML
                     MVTDVVRGSSRLLIANEPALFGDLPYPPHPDGTRLAEGVVSRKKQLLPVVLGLLES"
     gene            3785..4573
                     /locus_tag="AC16V7_15830"
     CDS             3785..4573
                     /locus_tag="AC16V7_15830"
                     /inference="COORDINATES: protein motif:HMM:NF024869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR6258642.1"
                     /translation="MIYKAIAELEHKNEAAALCTVVRSSGSTPRHAASKMLVYPDGHI
                     LGTVGGGELENRVFKEALQALKDGTPRILEYSMADPQRGDPGVCGGQLEVFVEPIMPK
                     PVLVVVGSGHVGKAVAHLAKWLGFRVVVSDDRPEFCSPEAVPDGDEFYPVPMAELPDH
                     LKITPWTYLVLTTRGVLIDVPGLPALLDTPATYIGIIGSKRRWATTRKTLLDNGFPED
                     KLSRVRSPIGLELNAETPEEIAVSIMAEIIMLRQGGDGSVMTEE"
     gene            4587..>5757
                     /locus_tag="AC16V7_15835"
     CDS             4587..>5757
                     /locus_tag="AC16V7_15835"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR6258643.1"
                     /translation="MWHDYFSVTSVDDAIKLLAERGESARIVAGATDLILEMERGVRK
                     GIDTLIDITRVPDLDQIVLDEGNLIHLGPLVTHNHCVASPLIVEKGLPLALAAWEVGA
                     PQIRNRGTVAGNLITASPANDTITPLMALGAKVTLRSSSGVRVVPLEDFYTGVRRTVM
                     RPEEMLVDIFFPAMQPNQRGTFIKLGLRRAQAISLVNVAMLLTFQDDRVEKAAITLGA
                     VAPTIIRAPQAETFLVGRTLDENNIEEASRLAEQASRPIDDVRSSADYRSEMVRICTL
                     RGLRSLAAGTEKGDYPAQPVLLWGTEYQGAAPELSTLAQHGADTPINTKINGMIYTFT
                     TGHDKTLLRLLREEAGLVGSKEGCAEGECGACTVFLDGVAVMSCLVPTPRAHGAEI"
BASE COUNT         1305 a         1469 c         1717 g         1266 t
ORIGIN      
        1 gcaggctgac tttgtcattg caggcgaata ccatgtcccg aaagtccagc aagcgcacat
       61 cgagcctcac gttgtgctga cttattggga cgaggatgac cgcctggtaa ttcgcaccag
      121 cacacaagtg cccttccatg ctcggcgcat actggcgcca gtgctcggat tgcctataaa
      181 acgtatccgt gtgatcaagc cgcgcatcgg tggcggattc ggtggcaagc aagaggtgct
      241 gattgaggat gtggcggcac atctgaccat cgccaccggg cgaccggtag tgtatgaata
      301 tactcgtgag gaagaattta tcgctgctcg atcgcgccat cccatgcgcg tgcagatgaa
      361 aactggcgtt aagaaagacg gcaccattac tgccaacgag atgcgcgtgc tcaccgatac
      421 aggcgcctac ggttgccatg ccctgaccgt taccggtaac actgggcata aatccatggc
      481 gctgtatgtg ggtgatggcc cgtaccgtca aaagccaaat atccgtttct atgcagacgt
      541 agtctacacc aaccatccgc ctgctggggc atatagaggc tacggggtgc ctcaggggta
      601 ttggccagta gagcgccaca tggagaagat tgcccgccag ctaaaccttg acccgctgga
      661 gttccgtttg aagaatgccc tgcgtgcggg tgagctgcac cccttcagca cggcctggag
      721 cgaaggccgc gagccgcgac ctgagaccat cgagacctgc gggttggaag aatgtgctcg
      781 ccaaggcaag gcttccattg gctgggacca gaagtatgga aatcttgaat ggcacaaccc
      841 ggatcctatg cgaccatatc tgaagaaagg tatcggtgta gcactggtaa tgcagggtac
      901 cgccattccc tatcttgaca tgggcggggc cagcatcaag atgaacgatg atggctcatt
      961 caacctgctg gtgggagcca ccgatttggg tactggatcg gatacggtgc tagcgcagat
     1021 ggctgccgaa gtgctgggcg tgccgttaga agatatgatc acctattctt cagacacaga
     1081 tttcactccc tttgataaag gggcttatgc ttccagtacc acctatatct cgggcgcggc
     1141 tgtagccaag gccgccgatc aagttgccca aaggattaag gcgcgcgccc tgaggatgat
     1201 caacgaggcc tcaagcgttg aaacgcgtca acccctggat gttggcgata tccgcctgca
     1261 ggatcgcaag gctatcgctc cagatgggcg ctctgtcacg ctagccgagg tcgctttgga
     1321 cgctctacat cacaaagacc aggaacaaat catgggtgtg gcctcgtacg tgtctccggt
     1381 ctcgccaccc cccttcgctg cccagtttgc tgaagtcacc gtggacacgg agaccggtca
     1441 ggtgcgggtg gataagctcg tcatggcggt cgattccggg attatcgtta accctgtcac
     1501 cgcctccggg caaattgagg gcggcatgac ccaggctttg ggctatgcgg tttgtgagga
     1561 aatgcggtat gatgagaaag gcagcgctcg tgagcgggat tttcgggatt atcatatctt
     1621 ccgtgcagat gaaatgcctg agctagagac catcttcgtg gagacgtttg agccttcaca
     1681 tccatttggg gtgaaggcgg cggccgagat ccccatggac ggcgtcgctc cagcggtggg
     1741 aaatgcggtg ctggatgcct gtggagcaaa tattgacgat aacccggtga tacctgaaaa
     1801 agtgtggcgg gcgttaagga cataaatata aatacgactc gggtgctttg atcggcgcgc
     1861 gggtcgaaga tttgtaaaaa agagagggca gaacaatgaa gggcaacgat aatctcatca
     1921 tggtcttgaa tgaactatta gctgatgaat tgacggcgat caaccagtac atggttcatt
     1981 ccgaaatgtg cgctgattgg cgctatgaaa aactgcatga ggctgccgaa gcgcgcgcta
     2041 tccaggagat gaagcacgca gaaaaattga ttgggcgcat cttgttcctg gaggggaagc
     2101 caaatgtgag caatctgaag cgcatccaca ttggaagcga tatcaaggag cagctcgtca
     2161 acgatcggat ggccgagcaa gaggcggttg tcgcgtataa caaaggcatt cagctggcgg
     2221 tcgatgtggc tgacaatggc agtcgagagt tgcttgaatc aatcttgaaa gatgaagagg
     2281 atcaccttga ttggttggaa gcccagcttg agcagattga gcaaatgggc atccagaatt
     2341 accttgtaga gcaaaccggt taaaccaaag gatatgatcg agcgcgtata cgtcattggg
     2401 cacgtaaatc cagacacaga ttccattgct gcagcagtgg gatacgcttg gctcttgcgc
     2461 gagcgagatg gagtggatac cgtcgccgct cgcgcagggg cgattaaccc ccagaccacc
     2521 tgggtgctca agcgcctggg gctggaaccg cccttgctgc ttactgatgc atcgccgcgc
     2581 tttgaagtgg tcactcgccg tttggatact gccagaccgg atagcctatt gcgggaagcc
     2641 tgggcgatca ccagccgaac tcgcggcctt gctccgattg tgggcgaaga cggcaccccc
     2701 tttggattga tcaccggcag cagcttgttc gattttgtgg ccaagctggt cggtacccat
     2761 ccccgtcgcc aggaaatgcg catcagcgag atccttgatc tgccttgcca ggaagcctgc
     2821 gataccaagg tgccgcgctt tcaggcgggt acgcgcattc gggatgtgat caaccgattg
     2881 ctgcgcgaag agcataacga tttcctgatt gtagacgaga agggattgta tctgggtgtt
     2941 tgccggcagc gcgacgcgat caatccgccg cgcctgcgca ttgtcctggt cgatcataat
     3001 gaacccgggc aagcacttgg ttctattgag gaagctgaac tgattgagat cctggatcat
     3061 caccgtcttg gcaacccgag cacgcacgtt cccatcaagt tcactgtgga tatcgtagga
     3121 agcacgagta ccctggtatc tgagcgtatg gaagaggcgg ggttgagtgc accacctgcc
     3181 ctggctggtt tgatgctggc agggctgctc tcggatacgc taattctcac ctcgcctacc
     3241 acaaccgaac gcgatagggg tgctgccgag cggctggctc gctgggcttt tgtacggaat
     3301 ggcccgctag ctggtgagaa tattcattcc tttggggagc aggtgatagg agctggtagt
     3361 gggctgtcta atcgcgatcc ggaggaggtc gttagttcgg atctcaagct atacgaagcc
     3421 gaagatgtgt cctttgccat tgctcaggtt gagatcacag acttaatgca gcttgacgaa
     3481 catctgacag cactcaaagg tgccctggag accttgcgcc agcggcgtgg tgtggatttt
     3541 gccatgctga tggtcaccga tgtggtgcgt ggctccagtc gcctgctgat tgccaacgaa
     3601 ccagcgcttt ttggcgattt accctatccc ccgcatccag atggcacgcg tctggcagag
     3661 ggtgtagtct cgcggaaaaa gcaattgttg ccagtagtat tagggctttt agaaagttga
     3721 gaatatgcga tgataatcga aaccttcaaa agcagatttt ctgtgtgaaa attgcgttca
     3781 aattatgatc tataaagcca ttgccgaatt agaacacaag aacgaagcgg cggcattatg
     3841 cacggtggtg cgaagtagtg gctctacccc gcgccatgct gccagcaaga tgctggtcta
     3901 tcctgatggg cacatactcg gcactgttgg tggcggtgag ctggagaacc gggtgtttaa
     3961 agaggctctt caagctctga aagatgggac accccgcata ttagaatata gcatggctga
     4021 cccgcagcgc ggcgatcctg gagtgtgcgg gggtcagctt gaagtttttg tggagccgat
     4081 catgccgaaa ccagtattgg tagtggtagg cagcggtcac gtgggcaaag ctgtggcgca
     4141 cttagccaaa tggctgggtt tccgggtggt ggtcagcgat gatcgcccgg agttttgcag
     4201 cccggaggcc gtgcccgatg gggatgaatt ctacccggtg ccaatggctg aattgccaga
     4261 tcacttgaag atcaccccct ggacctatct ggtgctgact actcgcgggg tgcttattga
     4321 tgtgcccgga ttaccagccc tgctggacac accagcgacc tatattggga tcatcggctc
     4381 gaaacgccgc tgggctacca cgcgtaagac gctgctcgat aatggtttcc cagaggataa
     4441 actgagccgg gtgcgatctc cgatcggttt ggagctgaac gctgagacgc cagaagagat
     4501 cgctgtcagc atcatggcgg agatcattat gctccgccag ggcggagacg gaagcgtgat
     4561 gaccgaggaa taaacctgga gattctatgt ggcatgatta tttcagtgtc accagtgtgg
     4621 atgatgcgat aaaacttctt gcggagcgcg gcgagagcgc acgcattgta gctggcgcca
     4681 ccgacctgat cctggagatg gaacgcggcg tccgcaaagg cattgatacc ttaatcgata
     4741 ttacccgcgt gccggatctc gaccagatcg tcctggatga aggcaacctg atccacttgg
     4801 gtccattagt tacccacaac cattgtgttg cttctccatt gatcgtcgag aagggacttc
     4861 cattggcgct ggctgcctgg gaagtaggcg ctccgcagat tcgcaaccgc ggcacagtgg
     4921 cgggcaatct aatcaccgcc tcaccagcaa acgataccat tactcccttg atggctttgg
     4981 gcgctaaggt aaccttgcga tccagctctg gagtacgcgt agtccccctg gaagattttt
     5041 acactggcgt gcggcgcacg gtgatgcgcc ctgaagagat gctggtggat atattcttcc
     5101 cggcgatgca acccaaccag cgaggcacat tcatcaaatt ggggctgcgg cgtgcccagg
     5161 caatctccct ggtgaatgta gcgatgctcc tgactttcca agacgaccgc gtggagaagg
     5221 ctgccatcac acttggggcg gtggctccca ccattatccg tgcaccacag gctgagacat
     5281 tcctagtggg tcgcacctta gatgaaaata acatcgaaga agcatctcgc cttgcagagc
     5341 aggcttcccg cccaattgac gatgtgcgca gctccgccga ttaccgcagt gagatggtgc
     5401 gtatctgcac attacgcgga ttgcgctctt tggccgccgg tacggagaag ggtgattacc
     5461 cggcgcagcc tgtcctcttg tggggcacgg agtatcaagg ggcagctcct gaattatcta
     5521 ccttggcgca gcatggcgca gatactccaa tcaacaccaa aatcaacggg atgatataca
     5581 cctttacgac cggacatgat aaaaccttgt tacgcctttt gcgcgaggaa gctggccttg
     5641 taggctccaa ggagggttgc gccgaagggg agtgtggggc gtgtacggtc tttttagacg
     5701 gggtagccgt gatgagctgc ctggtgccaa cgccgcgcgc acatggggct gagattg
//
LOCUS       DBNHOH010000876         3594 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_248259, whole genome shotgun sequence.
ACCESSION   DBNHOH010000876 DBNHOH010000000
VERSION     DBNHOH010000876.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_248259"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="AC16V7_15840"
     CDS             <1..1200
                     /locus_tag="AC16V7_15840"
                     /inference="COORDINATES: protein motif:HMM:NF024832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HIR6258644.1"
                     /translation="AGFGILGVITMFASVINRFASFRMGELVIKYVGYYTETGDRERA
                     AAIYKAAALVEMLASLVAFGLIWLLAPLGARYRAKDPNATGWFVIYGLIVLANLISES
                     STGLLQLFDRYRRMAALNVVQSLFTLAIIALAYLTGGGLLHILLAYLGGKALGALGLT
                     LAALLEATRRWGTGWWRVSISLLRPQVRELARFAVSTNISASLSLINKDSELLWVSFF
                     RNPLETGYYKTALAIVNLAQLPVSPLPQATYPELSRQAAHKDWAGMRHLLRQGSLLAG
                     GFTLLVSLGLILLGKPLILLLYKKPEFLPAYPALLILLAGFLVANTFYWNRSALLAIG
                     LPDFPAKVNLVLAALKVIGVLLLVPIYGYLASAALLAGSYLLGVTVSVIRFRAEIARQ
                     EQVGVAA"
     gene            1209..2360
                     /locus_tag="AC16V7_15845"
     CDS             1209..2360
                     /locus_tag="AC16V7_15845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR6258645.1"
                     /translation="MLRVAYVLPALLRPSGWRTYAIALLRSIQEYVEPVIFASAADAE
                     EARSLFPQKQVFVLPVTQSAALTSLRGQLALLRCYRAISSNAFPKVDLVHSLEAYPTG
                     LVGSWLSRRLGCPHALTAHGTYGVVWHDLFLDRLAYRRVLRHASLVLSVSQGTETMLR
                     RYFGSDLAGARLRVILNGNDFTKRVPQQQALERSWPEVPTMLSVGDIKPRKGQHHSLA
                     AFALVKKDLPQARYWIVGKYQNNEYYLNMQRYVAENDLKDVSFLGTVSDDELDRLYRL
                     ASLFVLTPQQEGLSFEGFGLVYLEAGAYGLPVVATRSGGVADAVKDGHTGLLVEPGDV
                     EGIAVALLRLLTNPDLLRRMGRENRLWAESLTWERNAAEHYQAYQEVCQ"
     gene            2357..3514
                     /locus_tag="AC16V7_15850"
     CDS             2357..3514
                     /locus_tag="AC16V7_15850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6258646.1"
                     /translation="MKIALVSPTHLPARRANTLQVMKMAQAMAGLGHVVRVAVPGFPS
                     EKVDWDDLAYQYGLQRQFPIEWLPVWPTMRRYDYGLRAVKWARGWGAELIYTRLPQAA
                     ALASILGMATIYEVHDFPPQGVATWLLRRFAQGRGARRLVCITRALAADLADRLGVPD
                     SPPFTVIAPDGVDLERYDDLPEPQRARQMLVGADHDYPLLQSYRFVAGYSGHLYAGRG
                     VELILDMAARLPEVGFLVVGGEPDESQALRSEVKSRWLENVIPVGFVPNAELPRYQAS
                     CDLFLMPYQRRVAASSGGDIARYLSPMKLFEYMACGRPILCSDLPVLREVLSPEIAVL
                     LPPDDVEAWVAAIQFLLNDPARCRSLGELVRAEAKGYTWEARASMMLEGIT"
BASE COUNT          672 a          966 c         1077 g          879 t
ORIGIN      
        1 gcgggtttcg gcatcctggg cgtgatcacg atgttcgcca gcgtgatcaa tcggttcgcc
       61 tcatttcgca tgggagagct ggtgatcaag tatgtgggct actatactga gacaggtgac
      121 cgggagcgcg cggcggcaat ttacaaggcc gccgcgctgg ttgagatgct ggcatccctg
      181 gtcgcttttg gcctgatttg gctattagcg cccttggggg cacgctatcg tgctaaagac
      241 cccaatgcta cggggtggtt tgtgatttac gggctgatcg tgctggcgaa tctgatttcc
      301 gagagctcta ccggattgct gcagctcttc gatcgctacc ggcgtatggc agccttaaac
      361 gttgtccaga gcctgtttac cctggcaatc atcgctctgg catacctcac tggaggcggt
      421 ttgctgcaca tcctgctagc ctatttaggc ggcaaggcgc tcggcgccct ggggctgacg
      481 ctggcagcgc tcttggaggc tacgcggcgc tggggcacag gttggtggcg cgtctctatc
      541 agcctattgc gaccgcaggt gcgcgagctg gctcgctttg ccgtcagcac caacatcagc
      601 gcctcgctca gcctgattaa caaagatagc gagctgctgt gggtctcgtt cttccgcaac
      661 ccgttggaga ccggctatta caagactgca ctggcgattg ttaacctggc gcaattaccg
      721 gtcagcccgt tgccacaagc cacttaccct gagctgtccc gacaggcagc ccataaagac
      781 tgggctggca tgcgccatct cctgcggcag ggatcattgc tggcaggagg ctttacactg
      841 ctcgtctccc tgggacttat tttgctgggt aagccattga ttctgttgct ctataaaaag
      901 cccgagttcc tgccagctta ccccgctctg ctcatcctcc tggcgggatt cttggtcgcc
      961 aatacctttt attggaaccg ttcagccctt ctggcgattg gtctgcccga ttttcccgcc
     1021 aaggtcaact tggtattggc tgcgcttaag gtgatcggcg tgctcctgct tgtccccatt
     1081 tacgggtatc tggcaagcgc tgccctgctg gcaggctcct acctactggg tgtcaccgtg
     1141 tcagtgatca ggtttcgcgc agagatagca cgccaggagc aagttggtgt ggctgcctag
     1201 aggttatctt gttgagagtc gcttacgttc tgcctgcatt gttgcgcccg tccgggtggc
     1261 gtacctatgc gatcgcgctt ttgcgatcta tacaggagta tgttgagcca gtgatcttcg
     1321 catcggctgc ggacgctgaa gaagcgcgtt ctctttttcc tcagaagcag gtgttcgtgc
     1381 tcccagtgac ccaaagcgcg gccttgacca gcctgcgcgg gcagctggcc ttgttgcgat
     1441 gctatcgcgc catatccagt aacgctttcc ccaaagttga tctagtgcac tctctggaag
     1501 cctatcccac aggacttgtg gggagctggc tttctcgccg tttggggtgt ccgcacgcgc
     1561 taactgctca tggaacatat ggggtggttt ggcacgacct cttcctggat cgcttggctt
     1621 accgccgtgt tctgcggcat gccagcctgg tgctctcggt ttctcaaggc acagagacca
     1681 tgctgcgccg atatttcggc tccgacctgg ctggcgcgcg cttgcgcgtg attttgaatg
     1741 gcaacgattt caccaaacgt gtgcctcagc agcaggcgct agagcgatcc tggcctgaag
     1801 tgccgacgat gctatctgtt ggtgatataa agcctcgtaa aggacaacac cacagcctgg
     1861 cagccttcgc actggttaag aaagacttac cacaggcgcg ttattggatt gtggggaaat
     1921 atcaaaataa cgagtattat ctgaatatgc agcggtacgt cgctgagaat gacctgaaag
     1981 atgtctcttt tctgggtact gtatcggatg acgagctgga ccggctttac cggctggcct
     2041 cgctgttcgt gcttaccccg cagcaagaag gattgagctt tgagggcttt gggctggttt
     2101 acctggaggc aggcgcctat ggcttgccgg tggtggcaac gcgcagcggc ggcgttgcgg
     2161 atgctgtgaa agatggccat actggcttgc tggtcgagcc gggggatgtg gaagggattg
     2221 ctgtagcgct gttgcgcctg ctgactaatc ctgatctgct gcgccgcatg ggtcgggaaa
     2281 accgtctgtg ggctgaaagt ttaacctggg aacgcaacgc agcagaacac tatcaagcgt
     2341 atcaagaagt atgccaatga agatcgcgct tgtttcaccc acccacctgc cggcgcggcg
     2401 cgctaatacc ctgcaagtga tgaagatggc tcaggccatg gctggcttgg gtcatgtggt
     2461 gcgggtggcg gtaccaggct ttccctcaga aaaggtcgat tgggacgatc tggcgtatca
     2521 atacggatta cagcgccagt tcccaattga atggctgccg gtttggccaa cgatgcgccg
     2581 ctatgattat ggtctgcggg cggtcaagtg ggcgcgtgga tggggggctg aattgatcta
     2641 cacacggctg ccgcaagcgg cggccctagc cagcatcttg gggatggcga caatctatga
     2701 agtccacgat tttccccctc aaggagttgc cacctggctg ttacgccgct tcgctcaagg
     2761 tcgcggggca cgccgactag tttgcattac ccgcgccctg gcggcagacc tggcggacag
     2821 gttgggcgta cctgattcgc cacctttcac ggtgatcgca cccgatggag ttgacctgga
     2881 gcgctacgat gatctgcccg aaccgcagcg agctcggcaa atgcttgtag gggcagatca
     2941 tgattatccc ctgctgcaat cctatcgctt cgtcgctggc tatagcggtc atttgtatgc
     3001 tggacgaggc gtggaattaa ttctagatat ggctgcccga ttgcctgaag taggttttct
     3061 tgtggtgggt ggcgagcctg acgagtcgca ggcgctgcga agcgaggtaa aatctaggtg
     3121 gctggagaat gtcattccag ttggatttgt gccgaatgct gagctgccgc gctaccaggc
     3181 ctcctgtgat ctgttcctga tgccctacca gcgccgggtg gcagcatctt ctggcggtga
     3241 tattgcacgt tatctgagtc caatgaaatt gtttgagtac atggcctgcg gacgtccaat
     3301 tttgtgtagc gatctgcctg tcctacgcga ggtgctttcc ccagagattg ccgtgctatt
     3361 gcctcctgat gacgtcgaag cctgggtagc cgcgatccaa tttctactga acgacccagc
     3421 ccgctgccgc tcactgggcg agctggtacg cgctgaagca aagggatata cctgggaggc
     3481 gcgcgcctcg atgatgttag aagggatcac ctaagtttgt tcactgaata tcgtggaatc
     3541 atgaagcctg aagcgcaaaa tagcaaacca gttctggctc aaagtagaga atcc
//
LOCUS       DBNHOH010000877         8023 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1722777, whole genome shotgun sequence.
ACCESSION   DBNHOH010000877 DBNHOH010000000
VERSION     DBNHOH010000877.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8023)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8023)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8023
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1722777"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1200
                     /locus_tag="AC16V7_15855"
     CDS             127..1200
                     /locus_tag="AC16V7_15855"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6258647.1"
                     /translation="MTDFISTASITLKNLSKYFDTLKAVDSVNLKVQAGTLVCLLGPS
                     GCGKTTTLRMVAGFEEPSEGHIFIGDDEITDVPPYARPTALVFQSYALFPHMSIYENI
                     AYGLRARRTPKNEIDQRVKEIIQLMELGGQEKKSPPQLSGGQQQRVALARALVIRPKV
                     LLFDEPLSNLDALLRVRMRSEIRGIQKQLGITSLYVTHDQEEAFSIADQVAIMNKGNL
                     IQLGTPRDLYRAPADRFVAEFVGLSNIVPVELVETNAHGATVSLFGKTIRSRRPPSNQ
                     NSPISVVLRPEALEIGKPDPTGVEAHVLTLAFTGPLVRYTIRTKDSTVLTVDLHNPGP
                     EQFFPEGTQVSLKLPEEVPALLG"
     gene            1208..1732
                     /locus_tag="AC16V7_15860"
     CDS             1208..1732
                     /locus_tag="AC16V7_15860"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6258648.1"
                     /translation="MSSKILAVCFDFGDTLADEDTEVKDDTQTTLRAELIPGAGEVVR
                     ELKQRGYQLALVADGRPGTYRNVLTQHGIYDLFDAFAISEELGVEKPNQRMFVHALDQ
                     LGIAPDDYKRTVMVGNYLERDIKGANEVGMISVWLDWAPRRPKIPKDESEIPHYTIKM
                     PIELLEVLENLENG"
     gene            complement(1807..2562)
                     /locus_tag="AC16V7_15865"
     CDS             complement(1807..2562)
                     /locus_tag="AC16V7_15865"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016972.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR6258649.1"
                     /translation="MSTQNPIQLIVFDIDGVLTEGETKALDLELLADLARLNRESRHN
                     PIFPAVTLCSGRPAQYVELMLQAIDGYLPGVFENGAGLYVPETYRFLPNPALSNNGKM
                     IHIIRQRLEETIIKSGEAYIQPGKEYSLTLFSSDPGRTSKLYDLVVAALGDLMREVKL
                     VYSTSCLNILPRQIDKGEGIRFLSEWTGYALSGMLGVGDSDVDQEFLSVVGYSAAPAN
                     SHPEIKAIVQYVSPFRTGEGVRDILAHFNIPLP"
     gene            2928..3920
                     /locus_tag="AC16V7_15870"
     CDS             2928..3920
                     /locus_tag="AC16V7_15870"
                     /inference="COORDINATES: protein motif:HMM:NF014110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258650.1"
                     /translation="MDNHIILATYDRGISRAERGSDGNWAVEQHATDYEVTCLAADPS
                     NPQTIFAGTRNGMLRSDDQGLTWYLSGMEGQIVKSLAVSPHDSNIIYAGTKPALMYIS
                     RDGGENWSELEGFRRIPNRWWWFSPAEPPDRRPYIISIAVSPSDPNVILAGIEFGAVV
                     RSADGGQSWSRHRTGALRDCHSLRFHATDGNWVYQAGGTGGGASLSQDGGLTFRKAKR
                     GLAKHYGIVCAADPERPDIWYVCVAPSPFNAFGEHPEIYLYRAKGDAGWEPIGWQPHP
                     LPVTPTALTTVHNKPAHLYAGLHNGDVWLTTDYGDSWTKMPFSFKGIWFSLLVI"
     gene            4143..6164
                     /locus_tag="AC16V7_15875"
     CDS             4143..6164
                     /locus_tag="AC16V7_15875"
                     /inference="COORDINATES: protein motif:HMM:NF016648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HIR6258651.1"
                     /translation="MMETNGKRNFPCLVILLAAGLVIVLACGVAATLGIGTFFWLTQR
                     TVTTSQSEPLQASSKINPQDAILTPTRGLVEIQDQAGGWSVLAAEMPISEGSRVRTGA
                     LSGAQLIFYNGSRATLWPNTEIAIEKLDFQDERQVVVLTQSTGESDHQVVSSPENKTR
                     YEVRTPAGSAEAKGTSFHVSVTPDQSAHFTVDEGSLAVSGQGESVVLEAGQTSAIYTD
                     HPPTEPALRISGEGVVAQTGATWVIDGQSYTVHEHTIIIGNPRVGDIVHVEGRLLADD
                     TRLADLIVLLRTSPANRFTLIGVVEEIDEQAWTVAGQTIEIGENADIDPSIQEDDLVR
                     VEGVILDNGALQAEKILPAGEEDDLQFEFTGVVETTGTSSWEISGIEVVIDGDTTIDD
                     ELKSGDLVRVRGIIQENGDWLARRITRAIEGDRFFEISGHIESIDPWKVAGVSFETRS
                     WTDIEPGLELGDLVRVKGQVQEDGTWVAHQIERIQDIPSPLIVIIGKVISTNPWVVSG
                     IPLNVTSETIIEGDIVPGMLVRVEISLLPDGTWQVLRITPLDEFTGIPGCTNLTATVI
                     SFDGNRLQLLGWPILVLGDEVKIDGKLRHNAVVVVQICFNEDGTFEVIQIIVIFLPEI
                     DVEPPPVEGHKVTICHKPYKKKGGNTINISRSALPAHLGHGDYVGACSR"
     gene            6418..7323
                     /locus_tag="AC16V7_15880"
     CDS             6418..7323
                     /locus_tag="AC16V7_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_110513544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HIR6258652.1"
                     /translation="MNSISMSDLLLIIFVLVDDWYQAYATDFRKGRPGKRPELSDSEV
                     MAMLLAMDFIPFPSERQFVAYLRANHLDLFPDLVDQSQFNRRARSVAPLLEQFRQAWL
                     IHLGVTLACAGLLDTKPIPVIGYRRSKQPSDFLGSAAYGCCASKKMHYFGYKLVTISS
                     LGGIPLIYDLVPANTDERQAAETLLDQLSGFHLFADKGFLGQAWQADIQRLTGNCVWT
                     PKRKNQAAQQPYAFERWLNGLRLRIEGLFNEIQNVGRNVERLLAKTVRGLCARIVAKM
                     TSHLVKYILRYNFGVDVLSFSWIDV"
     gene            7463..7930
                     /locus_tag="AC16V7_15885"
     CDS             7463..7930
                     /locus_tag="AC16V7_15885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258653.1"
                     /translation="MKHKIVFVTILTLALAMLISSCESAAAPSPTAPVDASPAPAEGV
                     PEYPAPAEGIPEYPAPAQAVPEYPAPQQAMPAYNPYPGPSESASNFIDWTQADALIQS
                     GEVAEVYQAQTLHVTLVLKDGSVALTIEPALDEVSKSIEQCGEACKDTKVVNQ"
BASE COUNT         1925 a         2172 c         2153 g         1773 t
ORIGIN      
        1 ggtgcgcggc tcatccagtg atggagcggc ctttgccggg ttattgatca tcatcatctt
       61 gatcgcgctg gggttgctaa gcaagctaac cggaaaaagc tttgtagatc tgtttagagt
      121 gtcctaatga ccgattttat atccactgcc tcaattaccc ttaaaaacct gagcaaatac
      181 ttcgatacgc tcaaagccgt tgacagcgta aacctgaagg ttcaggcggg cacactggtc
      241 tgcctgctgg gacccagcgg gtgcggtaaa acgaccactt tgcgcatggt ggctggtttt
      301 gaagaaccca gcgaggggca tatcttcatc ggcgatgatg agatcacaga tgtacctcca
      361 tatgcccgac cgaccgccct ggtttttcag agttacgccc tcttcccgca catgagcatt
      421 tacgagaaca tcgcttatgg actgcgggct cgaagaacgc caaagaacga gatcgatcaa
      481 cgtgtcaaag agatcatcca gctgatggag ttgggagggc aggagaagaa atcgcctcca
      541 caactctccg gtgggcaaca gcaacgtgtc gccctggcgc gtgccctggt catccggccc
      601 aaggtgctcc tgttcgacga gccgctctca aacctggatg ccctgctgcg agtgcgcatg
      661 cgcagcgaaa tcagggggat tcagaaacag cttggtatca ccagcctgta cgtcacccat
      721 gaccaggagg aagccttcag cattgccgat caggtcgcca tcatgaacaa agggaattta
      781 atccagctgg gtacaccccg cgatctatac cgcgctcctg ctgaccgatt cgtggcggaa
      841 tttgtcgggc tatcaaacat tgtccccgta gagttggtgg agacgaacgc ccacggcgcc
      901 accgtaagct tatttgggaa aacaattcgg agccggcgtc cacccagtaa tcagaacagt
      961 ccgatatcag tcgtcttgcg acccgaggcg ctggagatcg gcaaacccga tccaacaggc
     1021 gtcgaagcgc atgtgctgac cctggccttc accggtcccc tggtgcgcta cacaatccgc
     1081 acaaaggata gcaccgttct aaccgtcgat ctgcacaacc ccggcccaga gcaatttttc
     1141 cccgagggga cacaggtctc tctcaagctg ccggaagagg tcccggcgct gctgggatga
     1201 gaccggtatg agctcaaaga ttttagcggt gtgcttcgac ttcggtgaca ccctagccga
     1261 cgaagataca gaagtaaaag atgataccca gaccaccctg cgagccgagc tcattcccgg
     1321 cgccggagag gtggttcgtg aactgaaaca acgcggctac cagcttgccc tggtggctga
     1381 cgggcgacca gggacttacc gtaacgttct cactcaacac ggcatctacg atctttttga
     1441 tgccttcgcc atctcggaag aactgggggt agaaaaacca aaccagcgca tgttcgtcca
     1501 cgcgctggac cagctgggca tcgcaccgga cgactacaag cgcacagtca tggtgggtaa
     1561 ctatctggag cgagatatca agggcgccaa cgaagtgggg atgatcagtg tgtggctgga
     1621 ttgggctcct cgccgaccga agatccccaa ggacgagagc gagatacccc actacacaat
     1681 caagatgccc atcgaattgc tcgaagtgct ggaaaatcta gagaacgggt aacatcctca
     1741 tacccaggcg gtcggctcca actactggct ctcagcctcc attagactgc tctggggatc
     1801 attacctcaa gggagaggga tattaaaatg cgccaggata tcgcgcacgc cctccccggt
     1861 ccgaaagggg gatacatatt ggacaatagc cttgatttca ggatggctat tagcgggcgc
     1921 ggcgctgtag ccaacgactg acagaaattc ttgatccacg tcagagtcgc ctacccctag
     1981 catccccgac aatgcgtagc ccgtccactc agataaaaac cggatcccct cccccttatc
     2041 gatctggcgc gggaggatgt tcaaacaaga ggttgagtaa accagcttga cctcgcgcat
     2101 tagatcgccc agcgcagcaa caacgagatc ataaagctta ctcgtacgcc ctggatcgct
     2161 ggagaagagg gtcagcgaat attccttgcc gggttggatg taggcttcgc cagactttat
     2221 gatggtctct tccaggcgct ggcggatgat gtggatcatt ttgccgttgt tggagagggc
     2281 cgggttgggc aggaagcgat acgtttcagg tacatagagt ccggcaccgt tttcaaatac
     2341 acccggcagg taaccgtcga tagcttgcag catcaactcc acgtattggg ccgggcgccc
     2401 cgagcagagt gtcacggcgg ggaaaatcgg gttgtgacgg gattcccggt tcagccgggc
     2461 caggtcagcc aacaattcta agtcaagcgc cttggtttcc ccctcggtca gcacgccatc
     2521 gatatcgaag actatcagtt ggatagggtt ttgggtagac ataggaaact gattatatag
     2581 ccagcgctgc gacctgccaa ggttcgagat gtcaaaccta acctactaag aaaatgtagg
     2641 ggttccccct tgatggcgac taacttaaac tccacaacag actttttcgc cgcggattga
     2701 aatctgccgc ttaggatagg cacatttctt tgccccagag aattgctact ccattgagaa
     2761 ggagcactcc tttcccaatc aaatatcggc tcccaggatg ccgtacgggt gctcacttgt
     2821 ggcttggcgg aaatcaaaga cctgtcaggc atacgtgtat aatctagttg ggttataaat
     2881 ttagtgctca gctcacgggc atagtatccc atagggaata acaaccgatg gacaatcata
     2941 tcatcctggc tacatacgat agaggtatca gccgcgctga acgcggctct gatggcaact
     3001 gggctgtcga gcagcacgcc acagactatg aagtcacctg cctggcagcc gatccgtcga
     3061 atcctcaaac catatttgcg ggtacgagaa acggcatgtt gcgatccgat gaccagggtt
     3121 tgacctggta tctcagcggc atggaagggc aaatcgtcaa gtcgttggcc gtcagccccc
     3181 acgatagcaa catcatctat gctggaacaa aaccggccct gatgtatatc tctcgcgacg
     3241 gcggggaaaa ctggtcagaa ttggaaggct tcaggcgcat tcccaaccgc tggtggtggt
     3301 tctcgcccgc agaaccacct gatcgccgcc catacattat ttctatagca gtttctccct
     3361 cagacccaaa cgtgattctt gccggtatcg agttcggcgc ggtagtgcgc agcgcagacg
     3421 gcgggcaatc ctggtctcgc caccgcacag gcgccttgcg cgactgccat tcgctcaggt
     3481 ttcacgccac ggacggcaat tgggtctacc aggcgggcgg caccggtggt ggggcttcat
     3541 tgagccaaga cggcggcctc accttccgca aagcgaagcg cggtctggct aaacattatg
     3601 gcatcgtctg cgctgccgac ccagaaagac cagacatctg gtacgtttgc gttgcaccca
     3661 gcccatttaa cgcctttggc gagcacccag aaatctatct ttaccgggca aagggcgatg
     3721 ctggttggga gcctatcgga tggcaacccc atccacttcc cgtcacaccg acggctctca
     3781 ccacggttca caacaagccg gctcacctgt acgccgggct gcataacggc gacgtctggc
     3841 tgacgacaga ttatggtgac tcgtggacaa agatgccgtt cagctttaag ggcatatggt
     3901 tttcgttgct agttatctaa tctttcgata aacatatccg gaattctgta taggcaggtt
     3961 gtatctacct gatcactgaa caaaacgaac acagtttcgc atagctcgac tcccaaatac
     4021 aggctataaa aatctcaccg ccgaaactga tctcgaattt acgctttcgg gcttaatcat
     4081 tccttatctc tacccacacc catatacgat ataatccata taatgatctg tcgtaggagg
     4141 gaatgatgga aacaaatggc aaacgtaatt tcccttgctt ggtgatcctg cttgctgcag
     4201 ggcttgtgat cgttttagcc tgcggagtcg ctgccacgct cgggataggt acattctttt
     4261 ggcttacgca acgaactgtc acaaccagcc agtcagagcc gctccaggct tcatcaaaaa
     4321 taaaccccca ggatgccata ctgaccccca cccgcggcct ggttgaaatc caagaccaag
     4381 caggtggctg gtctgttctt gccgcggaga tgccgatctc ggaggggagt cgcgtgcgca
     4441 ccggggcgct atccggcgct cagctgatat tttacaatgg cagccgggcg acactttggc
     4501 ccaacaccga gatagcgatc gaaaaactgg atttccagga tgagcgccag gtggtcgtgc
     4561 tgacccagag cacaggcgaa agcgatcacc aggtggtgtc atctcccgag aataagacgc
     4621 gctacgaggt gcgtaccccg gcaggcagcg ccgaagccaa aggcacgtct ttccacgtca
     4681 gcgtcacgcc tgaccagtca gcgcatttca ctgttgacga aggctccctg gcggtaagcg
     4741 ggcagggcga atccgtcgtc ctggaggccg ggcagacgtc cgccatctac accgaccatc
     4801 cgcccaccga acccgccttg cggatttccg gcgagggggt ggtcgcccaa accggagcca
     4861 cctgggtgat cgacgggcag agctacaccg tccacgaaca caccatcatc atcggcaacc
     4921 cgcgggtcgg ggatatcgtg cacgtcgagg ggcggctgct ggcagacgat acgcgcctgg
     4981 ccgacctgat cgttttgctg cgcacctccc ctgccaaccg cttcaccctg attggtgtgg
     5041 tggaggagat cgacgagcaa gcctggacag tcgccggaca gacgatagaa attggagaga
     5101 acgcagatat cgatccttcg atccaggagg atgatctcgt cagggtggag ggcgtgatcc
     5161 tggataatgg cgccctacag gccgagaaaa tcctgcctgc tggagaggag gatgatcttc
     5221 agtttgaatt taccggcgtg gttgagacta ccggaacgag cagctgggag atttcaggga
     5281 ttgaggtcgt catcgatggg gacacaacca tcgacgacga actgaagtcc ggcgacctcg
     5341 tccgggtgcg cggcattatt caagaaaatg gagactggct cgccaggcgt atcacgcgag
     5401 ctatcgaagg cgatcgcttc ttcgagatct ccgggcacat tgagagtatc gatccctgga
     5461 aagtagcggg ggtttcattc gagacccggt cttggacaga catcgaacct ggactggagc
     5521 taggcgacct ggtgcgggtg aaaggccagg tccaggagga tggcacctgg gtggcccatc
     5581 agatcgagcg catccaagac attccatccc cgctcatcgt aatcattggc aaggtcatca
     5641 gcaccaatcc ctgggttgtc agcggcatcc cgttgaacgt cacttcggag acgatcatcg
     5701 aaggggatat cgtgcccggt atgctggtgc gggtcgaaat ctccctgctg ccagatggga
     5761 catggcaggt gctccggatc acgccgctgg atgaattcac cggcataccc ggatgcacga
     5821 acctgaccgc tacggtaatc agcttcgacg gaaatcgcct ccagctcctc ggttggccga
     5881 tccttgtgct gggtgatgaa gtaaaaatcg atggcaagct gagacacaac gctgtcgtcg
     5941 tggtccagat ctgcttcaat gaagatggca cttttgaggt catccagatc atcgtcatat
     6001 tcctgccgga gatcgatgtc gagccgccgc cggtggaggg gcataaagtg acgatctgcc
     6061 acaaaccgta taagaagaaa ggcggcaaca ccattaacat ctcgcgctct gccctgcccg
     6121 cccatctggg acatggggac tatgtcgggg cttgctcaag atgacgtgaa tatcccctga
     6181 ttttcacaag ggatgggaca acagcgtgac caaagcttcg gtcacgctgt tgcttttata
     6241 aactgagcca atcaccaatt aggtcagcat aagaatcatg accccttcgt atgacctccg
     6301 gcacttccaa acatgaccac atatttacta atatacggct gatgtgaatt aggttgacag
     6361 gaagggagaa actctccaag ataaaggtta gccaaccaat ccaaggagag cttccccatg
     6421 aatagtatat caatgagtga tcttctgttg atcatatttg ttctcgtaga tgactggtac
     6481 caagcctatg caaccgattt cagaaagggt cgacccggaa aacgacctga gttgagtgac
     6541 agtgaagtga tggcgatgct gctcgcgatg gatttcatcc cctttccaag tgaacggcag
     6601 tttgtggcct atcttcgcgc taatcatctg gacttatttc ccgacttggt ggatcaaagt
     6661 caattcaatc gacgagccag atcggttgct cccttgttag agcaatttcg tcaggcatgg
     6721 ctcattcatc ttggcgttac tttggcttgc gcaggtctgt tagataccaa gccgattcca
     6781 gtcatcggct atcggaggag caaacaaccc agcgattttc tcggcagtgc cgcttatggg
     6841 tgttgtgcca gcaagaaaat gcactatttc ggttacaaac tggttacgat ctcttcttta
     6901 ggcggtatcc cattgatcta cgatctggtg ccagccaata ccgatgagcg tcaagctgcc
     6961 gaaaccttgc tagatcagct ctcaggtttc catttgttcg ccgacaaagg ttttctgggt
     7021 caggcttggc aggccgatat ccagcgcttg accggcaatt gtgtctggac acccaaacgc
     7081 aagaaccaag ctgctcaaca gccttatgcg ttcgaacgtt ggctcaatgg cttacgtctg
     7141 cgcattgaag gattgttcaa cgaaatccag aacgtgggca ggaatgtcga acgtctattg
     7201 gctaagaccg tgcgcggttt atgtgcccgg attgtggcga aaatgaccag tcatttggta
     7261 aagtacatct tacgttacaa tttcggtgtc gatgtgcttt ccttctcttg gatagatgtc
     7321 taattcacat caagcgtata cttcaaaatt atgcagtaag aggagatagg ataatttttg
     7381 agagtgcgag ctgcccaaat taccctcatt taccctgcag ctgcatatgt cctatacttt
     7441 acatttcgtg caggaggact caatgaagca taaaattgtg tttgtcacca ttctgaccct
     7501 ggcattagca atgcttatat catcgtgcga gagcgccgct gccccctctc ctactgctcc
     7561 cgtggatgcc tctccggcgc ctgctgaagg tgttccagaa tatccagcgc ctgcggaagg
     7621 cattccggaa tatccagcac ctgcgcaggc tgttccggag tatccggcgc cgcagcaggc
     7681 gatgcccgct tacaatccat atcctggccc aagcgaaagc gccagcaatt tcatcgactg
     7741 gacccaggca gatgcattaa ttcagagcgg agaagttgcc gaggtatatc aagcccaaac
     7801 gctgcatgtc accctggtgt tgaaagatgg cagcgtggct ctcaccatag agcctgcact
     7861 agacgaagtg tcgaaatcta ttgagcaatg cggggaagct tgcaaggata caaaggtcgt
     7921 caatcaatag gcaaaatgaa ataatttcgt tcgcctcgaa gtagaatttt ctgcctgcat
     7981 tagcagattc attttggtat tgatgtttgt tgatgggttt atc
//
LOCUS       DBNHOH010000878         2846 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_505084, whole genome shotgun sequence.
ACCESSION   DBNHOH010000878 DBNHOH010000000
VERSION     DBNHOH010000878.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_505084"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="AC16V7_15890"
     CDS             complement(<1..1122)
                     /locus_tag="AC16V7_15890"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR6258654.1"
                     /translation="MLPQNWVGKTIGGRYQIESIIGQGGMSAVYKANDPNLRRVVAIK
                     LIHSHLSVNPNFVNRFKEEAAAVARLRHPNIVQVHDFNIDDDTYYMVMEYLVGETLQA
                     RLRRLTAAHRFLPCTDLIQFCIQLCDAIGYAHNHELIHRDIKPANIMLDLNGQAILMD
                     FGIVKIVGGDYHTATGATIGTAMYMSPEQIRSERVDERSDIYSLGVTLYEMISGQTPY
                     QADSAITLMMMVLNDPLPDLREIRKEIPENLLAVVEKALAKDPSERFQTMDEMAAALR
                     MAQEQLESTPSVATVVEDDELVQTLPLELPPEEHEKVQDSPKEPKLAEASLPEQQDVK
                     YSQRDIQRVEEGVSSIPTMKEAPIQPEIAPLPSAPSKIKG"
     gene            1405..1536
                     /locus_tag="AC16V7_15895"
     CDS             1405..1536
                     /locus_tag="AC16V7_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258655.1"
                     /translation="MKTSQEKLKAEFLAEAEALFDQLMEWDEHTAKPNLTQIEEIVL"
     gene            1573..1788
                     /locus_tag="AC16V7_15900"
     CDS             1573..1788
                     /locus_tag="AC16V7_15900"
                     /inference="COORDINATES: protein motif:HMM:NF014037.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HIR6258656.1"
                     /translation="MLARQENSQPAEKMRCPECRGELEPKGPKRNRVESQVGSLQMER
                     SYYYCPRCRQGFFPSGSTTTNMGKTLE"
     gene            1748..2761
                     /locus_tag="AC16V7_15905"
     CDS             1748..2761
                     /locus_tag="AC16V7_15905"
                     /inference="COORDINATES: protein motif:HMM:NF033572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258657.1"
                     /translation="MDQQLQIWEKHWSEQVAKQAVWLSGLVTFDQAEQILREVGRVDI
                     SQSSIWRRVDRWGRRLQAIETLQQVKAYGFEEPEGIDPNRPLGRMGVAMDGTMVHIRG
                     EGWKELKVGCVFNIAQRRQYDPHTQEVIELGSAVHNSYVAHLGGPEVFGRQLWAEARQ
                     RLWMYAEDSLTLGDGALWIWNLVGEHFYASHQLVDWYHASEHLASAAKTLNGEGTPAA
                     QRWYRQWETKLYQGHVDQLVQALEQQAEQQPDKAEALRKEAGYFHNNRKRMNYLEMRG
                     EGYLIGSGMVESAAKQYKARFTGPGMRWSRTGAEHLLPVRTAIMSGRFNKMWHLAYNS
                     PQI"
BASE COUNT          740 a          672 c          758 g          676 t
ORIGIN      
        1 tcccttaatt tttgatgggg cagatgggag gggagcaatt tctggctgga ttggcgcttc
       61 tttcatggtt gggatactgg aaactccctc ttccacccgt tgaatatcac gctgggagta
      121 tttaacgtct tgttgctccg gtagtgaagc ttctgccaac ttcggttctt ttggtgaatc
      181 ttgaaccttt tcgtgttcct ctggcggcaa ttcgagtggc aatgtttgta caagttcgtc
      241 atcctcgacc accgtggcta ccgagggagt gctttccagt tgctcttgcg ccattcgcag
      301 cgcagcagcc atctcatcca tcgtttggaa acgctcgctt ggatccttgg ccaatgcttt
      361 ttcaacgacc gccaacaggt tctcagggat ttccttccgg atttctcgta aatcgggtaa
      421 tgggtcattg agcaccatca tcatcagcgt tatggctgaa tcagcctggt aaggcgtttg
      481 cccactgatc atttcataaa gcgtcacacc caatgagtat atgtctgagc gctcatcaac
      541 gcgctcgctg cggatctgtt caggagacat gtacatcgct gtaccaattg tggctccagt
      601 agcggtgtga taatccccac ccacgatttt tacaatccca aaatccatca agatggcttg
      661 tccattcagg tcaagcataa tgttagctgg tttaatatct cgatggatca attcatggtt
      721 gtgagcatag ccgatggcat cacatagctg gatgcagaat tggatcagat ctgtacatgg
      781 caaaaaacga tgtgccgctg taagccgcct gagtctcgcc tgcagtgttt ctccaaccaa
      841 gtactccatg accatataat aggtatcatc atcgatattg aaatcgtgga cctgtacaat
      901 attaggatgg cgtagacggg cgaccgcagc tgcttcttct ttaaagcggt tgacaaagtt
      961 tgggtttaca gacaaatgtg agtgaatcag cttaattgct accacgcggc gcaagttggg
     1021 gtcattcgcc ttgtacacag cagacatacc cccttgccca ataatcgatt cgatctgata
     1081 tcgcccgccg attgtcttcc caacccaatt ttgcggcaac ataaatcctc accttagagt
     1141 tactcgagct acctcgagaa cccaaccccg atatggctag ttagggaaat ttttacagcg
     1201 gcgaaaccgc tgcaaaaacc ccccacctta cttaagagcg atctgcaata attatatata
     1261 ttttccgtat ttttagacaa cctactgggt aaataaccaa accaagtgta aataaaaccc
     1321 acatataaag cttgaaatgg gcgtatttca gttgtggggg aaaagtatgg cacaataagg
     1381 aaaataatgg tagcgaggtg agcgatgaag acaagtcaag aaaagctgaa agctgagttt
     1441 ctggcagagg ctgaagcgct ctttgatcaa ttaatggagt gggacgaaca taccgcgaaa
     1501 ccgaatttga cgcaaataga agagattgta ttatagttac gccaacagtt aggtgaacaa
     1561 ttggcccttg cggtgctggc ccgacaggag aatagccaac cggcagagaa aatgaggtgt
     1621 ccagaatgcc ggggcgagtt agaacccaaa gggccaaaaa ggaaccgagt tgaaagtcag
     1681 gtagggagcc tgcagatgga acgaagctat tactactgtc cccgctgtcg gcagggtttt
     1741 ttcccctctg gatcaacaac tacaaatatg ggaaaaacac tggagtgaac aggttgcgaa
     1801 acaggcggtg tggctgagcg ggttggtgac atttgaccaa gccgaacaga tcttgcggga
     1861 agtggggcgg gtcgatatct cgcaaagcag tatatggcgg agggtggatc gttgggggcg
     1921 cagactgcag gcgattgaaa ccttacaaca agtgaaggcc tatgggttcg aagagccgga
     1981 agggatcgat cccaaccgcc cattggggcg catgggagtt gctatggacg gtacgatggt
     2041 gcatatccgg ggagaaggtt ggaaggaatt gaaggttggt tgtgtgttta acattgccca
     2101 acgccgtcag tatgatcccc acacccagga agtgatcgag ctgggaagtg ctgttcataa
     2161 cagctatgta gctcatttgg ggggacccga agtatttggt cgccaactat gggccgaagc
     2221 ccggcagcga ctgtggatgt atgccgaaga tagcctgacc ctaggggatg gggccttgtg
     2281 gatctggaac ctggtggggg agcacttcta tgccagtcac caattggtgg attggtatca
     2341 tgccagcgag catctggcca gtgcagccaa gaccttgaat ggcgaaggca cccccgctgc
     2401 ccaacgctgg tatcgccagt gggaaaccaa actctatcaa ggccacgtcg accaactggt
     2461 acaggctctg gagcagcagg ctgaacagca gcccgacaag gcggaagcgc tacgcaagga
     2521 ggccggttac ttccacaaca accgcaaacg catgaattac ctggagatgc gtggtgaagg
     2581 ctatctgatc ggcagtggaa tggtggaaag tgcagctaag caatacaagg ctcgcttcac
     2641 tggccctggt atgcgctgga gccgaaccgg cgcagaacat ctcctgccgg tgcgaacggc
     2701 gatcatgagc ggtcgcttca acaaaatgtg gcatctggct tataactcac cccaaatttg
     2761 aaatgcgccc atccccagct cctttcagca aacatctttt gggtgtggta gaatccgtca
     2821 ccagatgtca ttggtaataa tccaaa
//
LOCUS       DBNHOH010000879         1803 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_568056, whole genome shotgun sequence.
ACCESSION   DBNHOH010000879 DBNHOH010000000
VERSION     DBNHOH010000879.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_568056"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="AC16V7_15910"
     CDS             <1..725
                     /locus_tag="AC16V7_15910"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6258658.1"
                     /translation="IHISSTAVYGVPIRHPIYEDDPLIGVGPYGVAKIEAEQVCLEFR
                     QHGLCVPILRPKSFVGPERLGVFAIFYEWASQGKNFPMIGMGHNRYQLLDVEDLCAAI
                     HQCMTLDEQVANDTFNIGAEEFTTMRRDYQAVLDEAGFGKHIIGFPAGPVVLALRLLE
                     KLKLSPLYPWIYETAFKDSFVSIDKAKEKLEWSPRYSNQDALVRNYRWYMEHGDEYRE
                     VTGITHRAPWKQKALKLVKVFF"
     gene            complement(868..>1803)
                     /locus_tag="AC16V7_15915"
     CDS             complement(868..>1803)
                     /locus_tag="AC16V7_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR6258659.1"
                     /translation="VLLIDKTGTLTLGKPQIVDCRLLMGDSSEQSTIDNLKSEILQYA
                     ASAERYSEHPLAEAVRQAARQRELSLYEPVDFQALPGKGVQARVDGHWIEVGREAAGQ
                     VTNSPYHELETQGKTPLFVKIDGELVGALVAADTLRPEVPQAIAELRNLGIRQIELLT
                     GDQPGAAASLAAPLDVPYRAGLLPEDKIRIVQEYQAKGHTVVMVGDGVNDAPAFAQAD
                     VGIAMGAAGSQVAIEAAHIALMRDDWSLVPQAYLIARRTLRVVKMNIAFTTIYNVIGL
                     SLASLGFLPPIFAAAAQSLPDLGILANSSRLLKQK"
BASE COUNT          394 a          530 c          498 g          381 t
ORIGIN      
        1 tcattcacat ctcctccaca gcggtgtacg gcgttccaat tcgccacccg atctacgagg
       61 acgacccact aatcggcgtc ggaccctacg gggtggccaa gatcgaggcg gagcaggtct
      121 gcctggaatt tcgccagcat ggtctgtgcg tgccgatcct gcgacccaag tctttcgtcg
      181 ggccggagcg cctgggcgta ttcgctattt tctacgagtg ggccagccag ggaaagaatt
      241 tccccatgat cgggatgggc cacaaccgct accagctcct ggacgttgaa gacctatgcg
      301 cagccattca ccagtgcatg acactcgacg agcaagtagc gaacgatacc ttcaacattg
      361 gcgccgaaga attcaccacc atgcgccggg attaccaggc cgtactggac gaggccggct
      421 tcggcaagca catcattggc ttccctgctg ggccagttgt actggctttg cgcctgctgg
      481 agaagctcaa gctctcacca ctctacccgt ggatctacga gactgctttc aaagattcgt
      541 tcgtctccat tgacaaggct aaagagaagc tggaatggtc gccgcgctac tctaaccagg
      601 atgccctggt gcgtaattac cgctggtaca tggagcatgg cgacgaatac cgtgaggtca
      661 ccggaatcac ccaccgcgcg ccgtggaagc agaaagcgct gaagctggta aaggtatttt
      721 tctaagcccc acccacccct aacccctccc tccccaatgg gagggagggg aaaagatcga
      781 aaaaactggt tatcggacgg cgagtgcgcc gtccgataac cagttttttc gttattgata
      841 cccccaaggg gataaaggag acaggcttta tttctgcttt agcaaccgcg aggaattcgc
      901 caggataccc aggtctggca gagattgggc cgctgcagca aagatgggcg gcaagaatcc
      961 caaagacgcc aggctcaagc caataacatt ataaattgtc gtaaaggcga tgttcatttt
     1021 caccacccgc aaggtgcgcc gggcgatgag ataagcctgt ggtaccagcg accagtcatc
     1081 gcgcatcagg gcgatatgcg ccgcttcgat cgccacctgg ctgcctgctg ctcccatagc
     1141 gatgcccaca tccgcttgag caaaggctgg tgcatcgttc acgccatctc caaccatcac
     1201 tacggtgtgt cccttggcct ggtactcctg cacaatacga attttgtctt ccggcagcaa
     1261 gccggcgcga tagggcacat ccaggggtgc tgccagggag gccgctgcgc ccggctggtc
     1321 accggtgagc aactcgatct gccgaatacc caaattgcgc agttccgcga tggcctgggg
     1381 tacctcaggg cggagggtat cggcggcgac cagcgctcca accagctcgc cgtcgatttt
     1441 gacgaataga ggcgtttttc cctgggtttc tagctcgtgg tagggcgagt tggttacttg
     1501 ccctgcggct tctctgccga cctcgatcca gtgaccatcc acgcgcgctt gcacgccctt
     1561 gccagggaga gcctgaaaat ccaccggttc gtacaagctc aattcacgct ggcgagctgc
     1621 ctggcgcact gcctcagcca gtgggtgttc ggaatagcgc tcagcggagg cggcatattg
     1681 gagaatttca gattttagat tgtcgattgt tgattgctca gacgaatcac ccatcagcaa
     1741 tctacaatct acaatttgtg gtttgcccag cgtcagggta cccgtcttat caatgagcag
     1801 cac
//
LOCUS       DBNHOH010000880         1692 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_312998, whole genome shotgun sequence.
ACCESSION   DBNHOH010000880 DBNHOH010000000
VERSION     DBNHOH010000880.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_312998"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1596)
                     /gene="trmFO"
                     /locus_tag="AC16V7_15920"
     CDS             complement(<1..1596)
                     /gene="trmFO"
                     /locus_tag="AC16V7_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013401268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HIR6258660.1"
                     /translation="MLQTPNSVNFVYEQIASLPMLNDHYTTRLVLVEELVSRCSFILY
                     IYKRDPGIVGILVQFYLEKIICMSDLKKIGKETGGERDRQLADLVVVGGGLAGSEAAW
                     QAAERGIQVTLYEMRPHVTTGAHAGGFLAELVCSNSLGSNLDDRASGLLKNELRRLRS
                     LLIKIADNAAVPAGGALAVDRQIFAQKVTEAIENHPNIHVIRQEVNDIPDGPVIIATG
                     PLTSPRFSDAIRRLTGSEHLFFYDAIAPIISKDSINMDIAYRASRYERGEQKVGDYIN
                     CPLTRDEYYDFVEALTNAERIELKVFESELEKGVRAGAERFFEGCLPVEILGQRGADT
                     LAYGPLRPVGLVDPRTGTRPYAVVQLRQDNLAASLYNMVGFQTNLKFSEQKRVFRMIP
                     GLEKVDIVRYGQMHRNTFIFSPAYLKPTMQFNRRNDLFFGGQITGVEGYVGNIATGLL
                     AGWNAARLLQGKETLVLPRTSMLGALCHYVTHASPEDFQPMKANFGILPELEGTNSKI
                     RGKRMRARAYTERAMRDLSEFLES"
     gene            1595..>1692
                     /locus_tag="AC16V7_15925"
     CDS             1595..>1692
                     /locus_tag="AC16V7_15925"
                     /inference="COORDINATES: protein motif:HMM:NF014470.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HIR6258661.1"
                     /translation="MPRLGPTELIIILVIIIVLFGVGRISKIAGELG"
BASE COUNT          444 a          462 c          336 g          450 t
ORIGIN      
        1 cgactcaaga aattcactaa gatctctcat ggctcgctca gtataagctc tcgctctcat
       61 gcgcttcccc cttatcttcg agttcgtccc ttcaagttcc ggtaagatgc caaaatttgc
      121 tttcatcggc tgaaaatcct ctgggctagc atgggttaca tagtggcaca atgcccccag
      181 catgcttgtt cgcggtagaa ccagcgtttc tttgccttgc aatagtcgcg cggcattcca
      241 tccggctagt aaacctgtag caatattgcc cacatatcct tccacacctg tgatttgacc
      301 gccaaaaaac aaatcatttc gacgattgaa ctgcattgtc ggcttcaagt acgctggaga
      361 aaagataaat gtattgcgat gcatttgccc gtatcgcaca atatcgacct tctcgagccc
      421 tgggatcatc cgaaaaaccc tcttctgctc cgaaaatttt aaattagttt gaaaccctac
      481 catgttgtat agggaggcag ccagattatc ctgacgtaat tgaacaaccg catatgggcg
      541 tgtccccgtt ctaggatcca ctaaacccac agggcgcaag gggccatatg caagggtatc
      601 cgctccgcgt tgccccagta tttcgaccgg cagacagccc tcaaaaaacc gctccgctcc
      661 agcgcgaacg cctttttcta gctcgctttc aaatactttc agctctatcc gctccgcatt
      721 ggtcaaggct tcgacaaaat cgtagtattc atccctggtc aaaggacaat taatataatc
      781 ccccactttt tgttcacccc tctcgtaccg cgatgcgcgg tatgcaatat ccatattgat
      841 agaatctttc gagatgatcg gagctatcgc atcataaaaa aataagtgct cgctgcctgt
      901 taatctccga atcgcatccg aaaatctagg cgacgtcagt ggcccagtag caataattac
      961 aggcccatca ggtatatcgt ttacttcctg acgtatcacg tggatatttg gatggttctc
     1021 aatggcctcg gtcacttttt gagcaaatat ttggcggtca accgccagcg cgccacctgc
     1081 tggcacagcc gcattatctg ctatcttgat tagcagcgac ctcaacctgc gcaactcatt
     1141 ctttaagagt ccactggctc ggtcatccag gttggaaccc agagaattgg aacatactag
     1201 ttcagcaaga aagccgcctg catgggcacc cgtagtcaca tgaggtcgca tctcatacaa
     1261 ggtcacttgg atcccgcgct ctgcagcctg ccatgcggct tcactgcccg ctaagccacc
     1321 acccacaact actaaatcgg cgagttgcct atccctctct ccacctgtct ctttaccaat
     1381 tttctttaaa tcagacatgc aaataatctt ctctaaataa aattgtacca aaatcccaac
     1441 aataccagga tcgcgtttat atatatagag aatgaacgaa catctactca ccaattcctc
     1501 aacgagcacc aatcgggtag tataatgatc gttaagcatg ggcaaagatg caatttgctc
     1561 atacacaaaa tttacagaat taggagtttg taacatgcct agacttggtc ccaccgagct
     1621 tatcattatt cttgtgatca tcatcgtgct gtttggagtt ggccgtatca gtaaaattgc
     1681 tggcgaatta gg
//
LOCUS       DBNHOH010000881         1567 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_505197, whole genome shotgun sequence.
ACCESSION   DBNHOH010000881 DBNHOH010000000
VERSION     DBNHOH010000881.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_505197"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1567
                     /locus_tag="AC16V7_15930"
     CDS             <1..>1567
                     /locus_tag="AC16V7_15930"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6258662.1"
                     /translation="NAVLIATGAHQAKKLPIRNSNHPGNWLSLKLLRSVALGENINLT
                     GKKVVVLGGGNVALDTARTVLRLGASQVRMACLEPRGEMPGFEWEVKVAEDEGVIFCP
                     ARTFKEIVVENEKISGVRCAEVIFHGFKRGRPDIEEISSTEHILPADLVIWAIGQGPD
                     FSFLPPDGSINTRYPVGIQSNMDMMTTQPGVFVAGDVHRGVTFFVVDAIGEGHKAARS
                     IDRYLKGRNNITEPSKLPTVELDLEEIRSKLAHGEASSKARIPIRSIPLEERRHNFRE
                     VDLTMSEEEALAEAERCLRCGICSECLECVVACERGAINHNMREETIELKVGTIILAT
                     GFTDFNPSQLSELGYGNLDNVLTAMEFERLINASGPTGGKVLLKNGQQAKSVAILHCI
                     GSRDVANNEYCSRACCMYSLKIAQLVNDYMEAEVHEVYRDMRTFGKDYEEFYNRTRQK
                     GINFHHGKVREISRRNGKLRVAWDENFYNQPDHVDVDMVVLATGFEPQSDTAKVASIF
                     GVSRSRDGFFLERH"
BASE COUNT          429 a          352 c          415 g          371 t
ORIGIN      
        1 aatgcggttt tgattgctac tggtgcacac caagccaaaa agctacctat acgaaactcc
       61 aaccatccag gcaactggct cagcctgaaa ttgcttcgaa gcgtcgccct tggcgagaat
      121 attaatctaa ccggcaagaa agtggttgtc ttaggtgggg gaaacgtcgc cctggatacc
      181 gcacgcactg tgctgcgctt gggagccagt caggttcgca tggcatgcct ggaaccgcgc
      241 ggtgagatgc ctggcttcga atgggaagta aaagttgctg aagatgaagg tgtgattttt
      301 tgccccgcca gaacattcaa agagatcgtt gtagaaaatg aaaaaatatc tggtgtacgc
      361 tgtgcagaag tgatattcca cggctttaaa cgtgggcgac cggacatcga ggagatttct
      421 agcaccgaac atatcttgcc tgccgatctg gtgatctggg caattggcca agggccagat
      481 ttttccttct tgccccctga tggcagtatc aacactagat acccagttgg catccaaagc
      541 aatatggaca tgatgaccac tcaaccaggt gtcttcgtag ctggcgatgt acatcggggt
      601 gtgacgttct ttgtggtaga tgccattgga gaaggacata aggctgcccg cagcatagat
      661 cgctatctta agggcaggaa taacattaca gaaccgtcca aactaccaac tgtcgagctt
      721 gatttggaag agattcgctc aaaactcgcc catggggagg cttcttcaaa agcacgtata
      781 cccattcgaa gtatccctct cgaagagagg agacacaact tccgcgaggt ggatctcacc
      841 atgagtgaag aggaggcgct ggctgaggca gagcgttgtc tgcgatgtgg aatttgctca
      901 gaatgcctgg agtgcgtggt agcttgcgaa cgtggggcaa ttaatcacaa tatgcgagag
      961 gaaaccatcg agttaaaagt tggcacgatt atcctggcaa ccggatttac ggatttcaat
     1021 ccatcacaac tttctgaact gggctatggc aatttggata atgttctcac cgcgatggag
     1081 ttcgaacggt taatcaacgc aagcggtcca acaggcggga aggtgcttct taaaaacggt
     1141 cagcaagcta agagtgtagc aatcttgcat tgcataggta gtcgcgacgt cgcaaacaac
     1201 gaatactgct cgcgggcttg ttgtatgtac tcgttaaaaa ttgctcaatt ggtaaatgac
     1261 tacatggagg ctgaagtcca tgaagtttat cgagacatgc gcactttcgg caaagattac
     1321 gaggaattct acaaccgcac gcgacagaaa ggcataaact ttcaccacgg taaagtaaga
     1381 gaaatttctc gtcgcaatgg caaactcagg gttgcctggg atgaaaattt ttataatcaa
     1441 ccagaccacg ttgatgtaga catggtcgta ctggctacgg gctttgaacc ccaaagtgac
     1501 acggccaagg ttgcaagcat ttttggggtt agccgtagcc gagatgggtt tttcctcgaa
     1561 cgccacc
//
LOCUS       DBNHOH010000882         2855 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_505263, whole genome shotgun sequence.
ACCESSION   DBNHOH010000882 DBNHOH010000000
VERSION     DBNHOH010000882.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_505263"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            351..2252
                     /locus_tag="AC16V7_15935"
     CDS             351..2252
                     /locus_tag="AC16V7_15935"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HIR6258663.1"
                     /translation="MLAGLEAYGASPATYTWFHDLRILLFAVISIITGIMIYLRKPSE
                     WITVYFSLVLVAMGVLISLELQSPPADLPPIYSLLINSAFTAFFFSFYIFPDGRFVPG
                     WTRWLIPGWVVTIVSVSLFSGTGLDLNSWPPLISVPIYLVLLGSSPAAQVYRFRKASN
                     TDQRQQIKLVVYGLTIFVTSFLGFWIPLMFASELQISGLTAVIYDLFGGTLLIIAFAV
                     VPLSIGAALLRYRLWDIDPIINRTLVYIVLTAMTIAIYVLLVGLLSTIFQMRSHPVFA
                     FLATGVVAVIFQPLRQRLQRVVNQFMYGERDNPYTVIARLGQRLGATLTPDAVLPTIV
                     ETVKDALKLPYCAIALQQDSGMEVVASDGVLSGMPVRLPLVYQNQAIGELVLATRSPG
                     EDFNPADRRLLEDLAGQAGIAAHSVGLITELQHARERLVITREEERRRLRRDLHDGLG
                     PHLASQTLTITAARQLLRQDPEAADQLLAEVVKHAQSAIDDIRRLVYDLRPPALDDLG
                     LIGALNARAKEFEQSGLQITIHVPEKLPPLPAAVETACYRIALEALTNVVHHAMARHC
                     TVSLNLKGKLCLEIIDDGCGLPVEYRAGVGLNSMRERAEELGGSFMIEPALHCGTRVV
                     VELPLAGDS"
     gene            2249..>2855
                     /locus_tag="AC16V7_15940"
     CDS             2249..>2855
                     /locus_tag="AC16V7_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6258664.1"
                     /translation="MTDKDKIRILIADDHPVFRFGLRALLQAQSEMEVIAEATSGEDV
                     VAQAAELQPDLILMDIHMPGMNGIQATRRILQTTPHIGILVITMFDDDSIFDAVRAGA
                     RGYLLKGADPDETVRAIRAVAHGEAIFSPKVAERLMSFFVHQQPASSSHVFPELTGRE
                     REILGLISQGYTNTAIADRLVISAKTVRNHVSNIYSKLQVAD"
BASE COUNT          609 a          764 c          740 g          742 t
ORIGIN      
        1 tgaagcagat acggcaggga gtaaccgctc agtttactcc ctgcttgact taccatattt
       61 atcaagttta aagtgtggct caagattcgg ttatgctact aagccactat gtctcaatat
      121 agcgcagacg ggctcttgca agcttacaac cctgtataat aacaaccatg ggcgcattga
      181 taaggaaccc aatcggcaaa tggcagttca gcaaacggct gcaatctatc ctgttgagaa
      241 ttatttgggg ttgcggtacg attgcacttc ttagcgtgtt aatattcatc acgacccaga
      301 tttttaagcc agagagcctc caatttcatt ctattttacc tgccgatctt gtgcttgccg
      361 gtctggaggc ttatggtgcc tcccctgcta catacacctg gttccatgat ctgcggatac
      421 ttctcttcgc cgtgatctcc atcataacag gcataatgat atatctgcgg aaacccagtg
      481 aatggattac ggtgtacttt tctttggttt tagttgccat gggggtgctt atctcattgg
      541 aactgcaatc ccctcctgcc gatctccctc ccatatatag tctgttgatc aacagcgcct
      601 tcacggcatt ctttttcagc ttctatatct tccccgatgg gcgctttgtg ccaggctgga
      661 cccgttggtt gattccaggc tgggtggtga cgattgtgtc tgtgtcatta ttttccggca
      721 cagggctaga tctcaatagc tggcctccat tgatcagtgt gccgatatac ctggtgcttc
      781 tgggaagctc accggctgcc caagtttatc gctttcgcaa ggcatccaac accgaccagc
      841 gccagcaaat taaactggtc gtttatggct tgacgatctt cgtgaccagt tttttgggat
      901 tttggatccc tctgatgttt gcctctgaat tgcagatttc cggattaaca gccgtcattt
      961 acgacctgtt cggtgggacg ctgctgatca tcgcgtttgc ggtggtgcca ctctcgattg
     1021 gtgcggcatt gttgcgttac cgcttgtggg acattgatcc aatcatcaac cgcactcttg
     1081 tgtacattgt cctaactgcc atgacgatcg ccatctatgt tttgttagtc gggctcttaa
     1141 gcacaatttt ccaaatgcgc agccatccag tatttgcctt tctggcaact ggcgtcgtcg
     1201 cagtgatatt ccagcctcta cgccagcggc tgcaacgggt tgtgaaccaa ttcatgtacg
     1261 gagagaggga taacccttat acggttatcg ctcgtcttgg gcagaggttg ggagctacct
     1321 tgacccccga tgctgtcctg cccactattg tagagacggt caaagatgcc cttaagctgc
     1381 cttactgtgc aattgcactg caacaagaca gcgggatgga ggtggttgcc agtgatggtg
     1441 tgctgtctgg catgcccgtc cggttgcccc tagtgtacca gaaccaagct ataggcgaat
     1501 tggtcttggc tacgcgttcc ccgggagagg attttaaccc tgctgacagg cgtctgcttg
     1561 aagatttggc aggccaggct gggattgccg cacactctgt gggcttgatt accgagctgc
     1621 aacatgctcg tgagcgtttg gtgatcaccc gggaggagga acgccgtcgc ttgcgtagag
     1681 acctgcacga tgggcttgga ccacacctgg ccagtcaaac actcaccatt accgccgcac
     1741 gtcagctgct tcgccaagac cctgaagccg ccgaccaact gctcgcagag gtcgtcaaac
     1801 acgctcaaag tgctatcgat gatattcgtc gtttggtcta cgacctgcgc ccgccagcgt
     1861 tggatgacct gggcttaatc ggtgccctaa acgcccgggc gaaagagttc gagcagagcg
     1921 gcttgcaaat taccatccac gtcccagaaa aacttccacc cctgccggcg gcagtcgaaa
     1981 cggcctgtta tcgcatcgct ctggaagctc tgaccaatgt agtccaccat gctatggcgc
     2041 gccattgcac ggtatccctg aatctaaaag gcaaactgtg tctagaaatc atcgacgatg
     2101 ggtgtggtct gccagtcgag tatcgtgcag gagtggggtt gaattcgatg cgcgagagag
     2161 ctgaagagct tggcggctca tttatgattg aaccggctct acattgcggc acgcgcgtgg
     2221 ttgtagagct gcctttggcg ggtgattcat gacagataag gacaagattc gtatcttgat
     2281 cgctgacgat caccctgtct tccgttttgg tttgcgcgcg ctcctgcaag cccagtccga
     2341 aatggaggtg atcgcagaag cgaccagtgg cgaggatgta gttgctcaag ccgcggagct
     2401 tcaacccgat ctgatcctga tggatattca catgccaggt atgaatggca tccaagctac
     2461 cagacggata ttacagacca ccccacatat tggcatactc gtgatcacga tgtttgacga
     2521 cgattcgatt ttcgatgccg tgcgcgccgg ggcgcgcggt tacctgctca agggggccga
     2581 tccagatgag acggtgcgcg ccatccgcgc cgttgcccat ggagaagcta tcttcagccc
     2641 caaggtggca gagcgcctga tgagcttttt cgtccaccag caacccgcct cctcctcgca
     2701 tgtctttccc gaattgaccg ggcgcgagcg tgaaatcctg ggtttaatct cgcaaggcta
     2761 caccaacaca gccattgccg accggttggt aatctctgcg aagactgtgc gcaatcacgt
     2821 ctcgaatatc tacagtaaat tgcaagttgc cgacc
//
LOCUS       DBNHOH010000883         2651 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_376874, whole genome shotgun sequence.
ACCESSION   DBNHOH010000883 DBNHOH010000000
VERSION     DBNHOH010000883.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_376874"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="AC16V7_15945"
     CDS             complement(<1..835)
                     /locus_tag="AC16V7_15945"
                     /inference="COORDINATES: protein motif:HMM:NF013242.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HIR6258665.1"
                     /translation="MSENSKPKLAMYWAAACGGCEIAVLNIGENILDVDAAFDVVFWP
                     VAVDFKYEDVRAYPDGYIDVCLFNGAIRTSENEEMAHLLRQKSKLLVAFGACANQGSI
                     PALANLTFPEDIFRAAYLQNPTTDNPSGVVPQEVTQVAEGELHLPEFYQTVMALDQVV
                     EVDYRIPGCPPEPHQIWAVLQTVVAALQGRVELPPRGAILGAGNVALCEECPLEKHEK
                     QITRFVRPYDIIPEPGLCLLEQELVCLGPATRSGCGALCPKVGMGCRGCYGALDKVSD
                     QG"
     gene            complement(901..1389)
                     /locus_tag="AC16V7_15950"
     CDS             complement(901..1389)
                     /locus_tag="AC16V7_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HIR6258666.1"
                     /translation="MSTKFEPVIIGFLCNWCSYRAADLAGTARIHYAPNLRPVRVMCS
                     GRVEPELVIKALRSGADGVLIAGCHPGECHYIEGNLKALRRYTLLEQMLAQFGIEPER
                     VQLVWASASEGPLLAEIVDRMTEQLRALGPLRWQQTVLGSGNGVGGHPRAQPVEREAA
                     VA"
     gene            complement(1432..>2651)
                     /locus_tag="AC16V7_15955"
     CDS             complement(1432..>2651)
                     /locus_tag="AC16V7_15955"
                     /inference="COORDINATES: protein motif:HMM:NF024634.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR6258667.1"
                     /translation="NVGLGYRKAVYRPFPQSVPMYPVIDVQNCTYFIKGKCKACEIFC
                     PAGGIDFDQKDELLELQVGSIILATGYSPFDARRIPQYGYGRLPNVYTSVEFERLVNA
                     SGPSAGRIVLRDGVTTPESVAIIHCVGSRDQNYNEYCSRVCCMYSLKFAHLVRERLPD
                     AEVYNFYIDIRAAGKRYEEFYHRVMMEGTHFIRGRVAEVTDAARIPEEEDKLIVQVYD
                     TLYGAQRRIPVDMVILSVGVEPRPDARKVAQLFGLGCDFSGFFIERHPKLDPILTMVE
                     GIFIAGACQGPMAIPETVAQGAAAAVRSAGLISQGTVLMEPVRASINADQCSGCRICN
                     NICPYSAIIFNEDRKVSEVVIAMCQGCGTCVAACPSAAIAGAHFTREQLMAQVEGLLW
                     DVQEIAVPQLAGD"
BASE COUNT          622 a          802 c          662 g          565 t
ORIGIN      
        1 ctccctggtc tgacaccttg tccaaagctc cgtagcaacc gcgacagccc atccctacct
       61 tcggacagag ggctccgcag cctgagcgag tggctggacc caaacagacc agttcctgtt
      121 ctaacaagca caaccctggc tcagggataa tgtcgtacgg gcgaacaaaa cgagttattt
      181 gcttctcgtg cttctccaag gggcattctt cacacagcgc cacattgccc gcacccagaa
      241 tcgccccccg aggcggcaat tcgaccctgc cttgcagggc agcgaccacg gtttgcagaa
      301 ctgcccaaat ctggtgaggc tcgggcgggc atcctggaat ccggtagtct acctccacca
      361 cctggtctaa tgccataacc gtctggtaaa actctggcag gtgcagctct ccttcagcca
      421 cctgggtcac ctcttgagga accacaccgc ttgggttgtc cgttgtgggg ttttgcagat
      481 aagctgcccg aaagatgtcc tcgggaaaag tcaagttggc cagcgccggt atacttcctt
      541 gattagcaca agcgccaaag gccaccagca gtttcgattt ctggcgtagc aggtgggcca
      601 tctcctcgtt ttccgaagtg cggatcgctc cattgaatag gcaaacgtcg atgtagccgt
      661 ctggataagc gcgcacgtcc tcatatttga agtcgacagc caccggccaa aagacgacat
      721 caaaggccgc gtcaacgtcc aggatatttt caccaatatt gagcactgcg atttcgcaac
      781 cgccgcacgc ggctgcccaa tacatggcta atttcggttt ggaattttca gacatggttg
      841 cacctgttga agagatagat catccgattg gcattcataa aataatccaa ggcgataaat
      901 tcaggcaacg gcagcctccc tttcaacagg ctgggcacgc ggatgtccgc caacaccatt
      961 cccactgcct aaaactgtct gctgccagcg tagcggccct aaagcccgca attgctcggt
     1021 catacggtcc acgatctccg ccagcagcgg tccttctgag gcagaagccc ataccaactg
     1081 cacccgctct ggctcgatgc caaattgagc cagcatctgc tccagcaggg tgtagcgtcg
     1141 caaggctttt agattgccct cgatataatg gcactctccc ggatgacaac cggcgatcaa
     1201 tacgccatca gctccagagc gcaatgcttt aattaccagc tcaggctcga cccgaccact
     1261 gcacatcacc cgcactgggc gcaaattcgg cgcatagtga atgcgggctg tgccagctag
     1321 gtcagctgcc cggtaggagc accagttgca cagaaaacca atgatgacag gttcgaattt
     1381 cgtgctcatg ggtcttcctc cttaaattgt ttctcgacaa gcatgcacat gttaatcccc
     1441 agccaactgt ggcacagcga tctcctgcac gtcccacaac aacccttcga cctgtgccat
     1501 caactgttcg cgggtgaaat gagctcctgc gatcgccgcg ctggggcagg ccgcaacaca
     1561 ggtgccgcac ccctggcaca tggcgatgac cacctcagat actttgcgat cctcattaaa
     1621 gatgatggcg ctatacgggc atatattgtt gcagatgcgg cagccggagc attgatctgc
     1681 gttaatggaa gctcgcacag gttccatcag caccgtaccc tggctgatta atcctgccga
     1741 acgaaccgcg gctgccgcgc cttgggctac tgtctctgga atagccatcg gcccctggca
     1801 agcccctgcg atgaaaatgc cttccaccat cgtcaggatg gggtccagct tgggatggcg
     1861 ctcgataaag aaaccactga aatcgcagcc caaaccgaaa agttgggcca ctttacgagc
     1921 gtcgggacgc ggctccacac ccacgctgag gatgaccata tccaccggta tgcgacgttg
     1981 tgcgccgtac agtgtgtcat agacctggac gatcagctta tcttcctctt cagggatgcg
     2041 tgcggcatcg gttacctccg ctacacggcc gcgaataaaa tgcgtgccct ccatcatcac
     2101 ccggtgatag aactcctcgt accgcttgcc agctgcccga atatcaatat agaagttata
     2161 aacttccgca tccggtagcc gctcgcgaac caggtgggca aacttcaaag aatacatgca
     2221 gcatacgcgg gaacagtact cgttataatt ctggtcgcgt gagcccacgc agtggatgat
     2281 ggctacgctt tcaggcgtgg tcactccatc tcgcaagacg atccttcctg cggatggccc
     2341 ggatgcgttc accaggcgct caaactcgac gctggtatat acgtttggca ggcgaccata
     2401 accatattgt ggtatacggc gggcatcgaa cgggctatat ccggtggcca ggataatgct
     2461 tcccacctgt agctccagca gttcatcctt ttgatcgaag tcaatcccgc cagcaggaca
     2521 aaagatctcg cacgccttac atttcccctt gataaagtac gtacaattct gcacatcaat
     2581 gaccgggtac atgggaacag actgaggaaa cgggcggtat actgccttac gataccccag
     2641 gccaacattg a
//
LOCUS       DBNHOH010000884         3532 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1787735, whole genome shotgun sequence.
ACCESSION   DBNHOH010000884 DBNHOH010000000
VERSION     DBNHOH010000884.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1787735"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="AC16V7_15960"
     CDS             <1..180
                     /locus_tag="AC16V7_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258668.1"
                     /translation="GEPIIAMNVQYRFKVIKMHHMFMVLGMLIAFFGLMLFVQVMDRS
                     YVTPEVYPNFGRFVI"
     gene            195..1430
                     /locus_tag="AC16V7_15965"
     CDS             195..1430
                     /locus_tag="AC16V7_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase-like domain-containing protein"
                     /protein_id="HIR6258669.1"
                     /translation="MHTPWDHRYAQRTQRMGSSAIRELLKLTEKPDIISFGGGLPAPE
                     VFPVEEFHAACERVLRDHGAQALQYSTTEGYQPLREQIARHTSRYHIQINPENVMLTS
                     GSQQALDLLGKIFINPGDHILVEEPTYLGALQAWSAYGAEYVPVPADKCGMNTDYLEE
                     ALRAGPKFIYVLPNFQNPTGVTLSLERRQKLVELADRYGVPIVEDDPYGQLRYEGEHL
                     PAVVTLDDQFRNNKGARYHGNVIYLSTFSKILSPGLRLGWVVAPEEVIRKLVQAKQGA
                     DLHTATFNQLVAYEVSRGGFLDQHIKVIRDVYKERLSVMLSAMDRFFPAEVDWTQPSG
                     GLFLWGIMPEYLNSEDVLRDALEKKVAFVPGESFFPRGGGQNTMRINFSNATPEKIQQ
                     GISRLGEVLLEKIRQKATA"
     gene            1685..2074
                     /gene="aroH"
                     /locus_tag="AC16V7_15970"
     CDS             1685..2074
                     /gene="aroH"
                     /locus_tag="AC16V7_15970"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HIR6258670.1"
                     /translation="MSVRGLRGATVAREDRPEVILTATRELLEAILEANPSLELDDMA
                     SALFTVSADLRSVYPAQAARQMGWQLVPLMCAQEIPVPGSLERCIRVLIHWNTNLPPE
                     AVRHVYLHGAASLRPDLAGEQSSQRRQ"
     gene            2076..3125
                     /gene="aroF"
                     /locus_tag="AC16V7_15975"
     CDS             2076..3125
                     /gene="aroF"
                     /locus_tag="AC16V7_15975"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR6258671.1"
                     /translation="MMIIMRSDATEEQIALVVERVETNDLRAHLSKGAERTVIGAIGD
                     GRPVNPDQFVFLPGVDRVLPISRPYKLASREFSQENTVFPLNGVSIGGDEIIVMAGPC
                     AVEDRSQLLETAIAVREAGAHVLRGGAYKPRTSPYSFQGLGEKGLELLAEARQITGLP
                     VVTEVMSPEQVPLVTSYADVLQIGARNMQNYALLHAAGASQYPVLLKRGMSATVEELL
                     MAAEYILSHGNRRVILCERGIRTFETATRNTTDINAIPVLKAMTHLPVILDPSHSTGY
                     WSYVTAIARAGVAAGADGLMVEVHTHPDQAFSDGGQSLKPERFAELVRQVRSVAQAVG
                     RTMETSKAYSNSVKA"
     gene            3131..>3532
                     /locus_tag="AC16V7_15980"
     CDS             3131..>3532
                     /locus_tag="AC16V7_15980"
                     /inference="COORDINATES: protein motif:HMM:NF014234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0033730 - arogenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - L-tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HIR6258672.1"
                     /translation="MDGDDFLSTSNIAIVGLGLMGGSLAMALKGHCATLRGLDSDDMV
                     ISQALERKVVDTASSHPKAALSGADIIILATPVGAIIDFIHHLPEFHPGPAVVLDIGS
                     TKTEITSALEALPQGFDPIGGHPMCGSEKSGL"
BASE COUNT          834 a          849 c          982 g          867 t
ORIGIN      
        1 ggtgaaccga taattgccat gaacgtacaa tatcggttca aagtaataaa aatgcatcac
       61 atgtttatgg tacttggtat gctcatagct ttttttggcc tgatgctttt tgttcaggtg
      121 atggacagat cgtatgtaac tcccgaagtt tacccaaact tcgggagatt tgttatataa
      181 aggagaaaca ctctatgcat actccatggg atcatcgcta tgcacagcga acccaaagga
      241 tgggcagctc cgcaattcgc gagctgttga aactaactga aaaaccggat attatttcat
      301 ttggtggggg tttacctgct ccagaagtct tcccagtaga agaatttcac gctgcctgtg
      361 aacgggtttt gagggatcat ggcgcacaag cattgcagta cagcacaaca gaaggttatc
      421 agccattaag agagcagatc gcccgccata catcccgtta ccatatacaa attaacccag
      481 agaatgtaat gcttacatcg ggatcacaac aagctttgga tctgttggga aagattttca
      541 tcaatccggg tgatcatata ctggttgaag aacctactta tttaggtgcc ttacaggctt
      601 ggagtgctta cggggcggag tatgtcccgg tgccagcaga taagtgcggg atgaacacag
      661 attatcttga ggaagccctg cgcgcgggac caaaatttat ttatgttctg ccgaatttcc
      721 aaaacccgac tggcgtcacc ctctcgttag agcggcggca aaagctggtt gagctggctg
      781 accgctatgg cgtacccatc gtcgaagatg acccttacgg tcagttgcgt tacgagggag
      841 agcacctgcc tgctgtggtg acactggatg accaatttcg gaataacaag ggtgcacgct
      901 atcacggtaa tgtaatctat ctgagcacat tctctaagat attatcgccc gggttgcgct
      961 tgggttgggt agtcgccccg gaggaggtga ttcgcaagct tgtgcaggca aagcaagggg
     1021 cagatttgca tactgccacc ttcaatcagc ttgtcgctta cgaggtatcg cgtggcggtt
     1081 tcctggatca gcacatcaag gtgatccgcg atgtgtacaa agagcggctg agtgtaatgc
     1141 tgtcagccat ggatcgtttc tttccggcag aagtggattg gacgcagcct tctggcggcc
     1201 tgtttttgtg gggcattatg ccagagtatt tgaactcaga agatgtgttg cgggatgcat
     1261 tagagaagaa ggtggcgttt gtgcccggcg aatctttttt cccaagaggc ggcggtcaaa
     1321 acaccatgcg catcaacttc tcgaatgcta caccggagaa aatacagcag ggaatttctc
     1381 gcctgggaga ggttttactc gagaagatta gacaaaaagc aacggcttga aaggtgtcgg
     1441 tttaaaaatt gcttcttgac gattatgcat tctttagata aacttggagc aattggtgat
     1501 aaatcctatc ccaaatcgaa cattgcgatg actttcttct cgaccctgtt ttttgtgcgc
     1561 ttttattttt acggcttcct ggcacaggtt gccgttggcg gcgcttagca gggtagagta
     1621 cattttcaaa cccaaaacgc gagaccaacg gtctcgcgtt tttgattatc tggaggatgt
     1681 gtacatgtct gtacgaggtt tgcggggtgc aactgtggca cgagaggatc gacccgaagt
     1741 tatcctgact gcgacaaggg agctgctgga ggcaatcctg gaagccaatc cctctctcga
     1801 gctggacgat atggccagcg cccttttcac cgtctctgca gatttacgca gcgtttaccc
     1861 ggcccaagca gcccgacaga tgggctggca actggtcccg ttgatgtgcg cccaagagat
     1921 tcccgtcccg ggcagtttgg agcgctgtat tcgggtgctg attcactgga ataccaacct
     1981 gccgcccgag gcggtcaggc atgtgtatct acacggagcg gcaagtcttc gccctgattt
     2041 agcaggtgaa caatcttctc aaaggaggca atagaatgat gatcatcatg cgttctgacg
     2101 cgaccgagga acaaatagcg ctggtggttg agcgagtaga gacgaacgac ctgcgcgccc
     2161 acctgtccaa aggtgccgag cgaactgtca tcggcgccat cggtgatggg aggccggtta
     2221 atccggatca attcgtgttt ttacctggtg ttgaccgggt gctgccgatc tcccggcctt
     2281 ataagctggc ctccagggag ttcagccagg aaaacacggt atttccacta aatggggtat
     2341 ccatcggtgg cgatgaaatc atcgtcatgg cagggccgtg cgcagtagaa gaccgctcgc
     2401 agttattgga gactgccatt gcggttcgag aagctggcgc tcacgtcctg cgcggtgggg
     2461 catataaacc gcgcacatcc ccctattcct ttcagggttt gggagaaaag ggattggagt
     2521 tgttggcgga ggcgcgccag atcactgggt tgccggtagt gaccgaggtg atgtcaccag
     2581 aacaggtgcc attagtgaca agctatgctg acgtgttaca aatcggcgcg cgtaacatgc
     2641 aaaattatgc gctgttgcac gcagctggtg ccagccagta tcctgttttg ttgaagcgcg
     2701 gcatgtccgc cacggtggaa gagctgttga tggcagccga atacatcctc tcgcacggca
     2761 accggcgagt aatcctgtgc gagcgcggca tccggacttt cgagaccgcc acacgcaaca
     2821 caaccgacat taacgccatt ccggtgctca aggcgatgac ccacctgcct gtcattcttg
     2881 acccaagtca cagcactggt tattggtcat acgtgactgc catcgcgcgc gcgggagtgg
     2941 ctgctggcgc ggatggactg atggtggaag tccacactca tcctgatcaa gctttctcag
     3001 atggcggaca atccttaaaa cctgagagat tcgccgaatt ggttcgccag gtgcgatctg
     3061 tggcacaagc agtgggtcgt accatggaaa cgtcgaaagc ctactccaac tcggtcaagg
     3121 cttgatcgta atggacgggg atgattttct ctcgacatcg aacatagcta tcgttgggtt
     3181 aggcttgatg ggtgggtcgc tggccatggc gcttaaggga cattgcgcga cccttagagg
     3241 cctggattct gatgacatgg tcatctcgca ggcattggag cgcaaagtgg tggacacagc
     3301 atcttcccac cccaaagcag cgctttccgg cgcagatatt atcatcctgg caaccccggt
     3361 tggtgcaatt atcgatttta ttcatcattt gccagagttt catcccgggc cggcggttgt
     3421 actggacatt ggctctacta aaactgagat cactagcgcc ttggaggcct taccgcaagg
     3481 ttttgaccca ataggagggc atccgatgtg cggtagcgaa aaatctggtt tg
//
LOCUS       DBNHOH010000885         3790 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1274086, whole genome shotgun sequence.
ACCESSION   DBNHOH010000885 DBNHOH010000000
VERSION     DBNHOH010000885.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1274086"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="AC16V7_15985"
     CDS             complement(<1..1216)
                     /locus_tag="AC16V7_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR6258673.1"
                     /translation="MVKSSKSPKPPSEDRTQPHHEDRENGEITSPLSLDKMGDLLAEI
                     QEDSIIEEPDDTALTLEAEELESLVKEEPQDILDDPKLIMELSDDPVRLYLKEIGGIK
                     LLDADEEFWLSTRLETVRRIDSLSRSHPLARRGTSVPKNIYRALFDELYFAWKRVQED
                     VKRLKHSAPDLLLVLAEAQTLRRSWNIQEPSYLRSFLDNGLWGTDPLWDGVARNAFTV
                     YLCLYSLPDGLAEFLEQDLKKRGNLPKESALLRRIPEDDILKAELEDIQMRANEAHHA
                     VIRANLRLVVSVAKRYIGRGSSFLDLIQEGNIGLLRAVSKFDPTRGYKFSTYATWWIR
                     QSISRSIADQARTIRIPVHIFESINRLIRVQRRLVQQLGREPTNEELAVEAGYLDGAD
                     QQNIARAQAEGTP"
     gene            complement(1227..3179)
                     /gene="dnaG"
                     /locus_tag="AC16V7_15990"
     CDS             complement(1227..3179)
                     /gene="dnaG"
                     /locus_tag="AC16V7_15990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR6258674.1"
                     /translation="MSTIDEIKARLDIIDLVSESVQLRRSGKNYTGFCPFHSNTRTPA
                     FVVFPETGTWRCFGQCNEGGDIFSYVMKKEGWDFAETLRFLADKAGVELKPVTPEEQA
                     AAEEHDSLRLLLEEAVTFYRHQLLNTPAGQQALAYLHQRGLNDETIEAFDLGYAPQSW
                     DATLSYFKSKNYTDDDLLAVGLLTQSSQSEAEGGSQHPARIYDRFRHRIMFPIRDERG
                     RMAGFGARILDPNDIPKFLNSPQTAIFDKGQLLYGLDRARKPIRKVDQAVIVEGYLDV
                     IALHQAGFDNAISPMGTALTERHLYLLKRYTRRIVMALDPDAAGSQATLRGLQIARQS
                     LDREQELVFDARGLLGFEARLQADIRVTTLPEGMDPDDVVNRDPQEWQRILEHAKPVV
                     LHVMETLAAQQDLDDPKVKSQIAAQVLPLINDLPSAIERDTYRQRLARILKLDERTLS
                     GEVRSSRLRPPRRAPQKLKTAEPVETPDTSKTTSVSHRREAHALGVLLRRPDLLYQVN
                     RHLQESGLSRLTEDDFQHADHQAILHLLLESVDQDVAEPLNYVLNSLSLDLMEVADRL
                     IAPTAKLDPNDERVLADLVRAILDLRERHLKQFINHLRFLQEEAQQSGDLMAVEYGQI
                     VVTNSRILNQLNKALGKYSDRVIVNR"
     gene            3257..>3790
                     /locus_tag="AC16V7_15995"
     CDS             3257..>3790
                     /locus_tag="AC16V7_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010565120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR6258675.1"
                     /translation="MMTTSQNSPEGYISVDVETAGPNPSQYSLLTIGACTLSGSPRTF
                     YVELKPVNMNAIPEALAASRLSMEHLAERGLEPAEAMLRFEDWLKVEVPSGQKPLFVS
                     FNAPFDWMFVNDYFYRFLGRNPFGHTAVDIKAYYMGLTGVNWDQTSMKILGPRYLGNR
                     ELTHHALRDAMDQAELFR"
BASE COUNT          850 a          992 c         1038 g          910 t
ORIGIN      
        1 ggggcgtgcc ttcggcctgg gcgcgggcaa tgttctgctg gtcggctcca tcaagatagc
       61 cagcttcaac agccagctcc tcgttggtgg gttcgcggcc tagttgctgg actaatcgcc
      121 tctgaacgcg aatcaggcgg tttatagact caaagatgtg tacagggata cggatggtgc
      181 gcgcctggtc agcgattgat cggctgatag actggcgaat ccaccaggtg gcataggtac
      241 tgaatttata gccgcgcgtc ggatcaaact tggatacagc ccgcagcaat ccaatgttcc
      301 cttcctggat tagatccaga aaagaactgc cccgcccaat gtagcgtttg gccacgctaa
      361 ctaccaggcg cagattggcg cgaatgacag catggtgggc ttcgtttgcg cgcatttgga
      421 tatcttcaag ttcagccttc aagatatcat cttcgggtat acgccgcagt agagcgctct
      481 ccttgggcaa attaccacgc ttcttgagat cttgttctaa aaattcagcc agcccatccg
      541 ggagagaata taggcagaga tagacggtaa aggcattgcg tgccacgccg tcccaaagcg
      601 ggtccgtgcc ccaaagtccg ttgtccagaa aacttcgcag gtatgaaggt tcttggatat
      661 tccatgatcg gcggagagtc tgggcttcgg cgagtacaag cagtaaatcc ggagcactgt
      721 gcttcagccg cttgacatct tcctgtacgc gcttccaagc gaagtacagt tcgtcgaaga
      781 gagcccgata aatatttttc ggtacagagg tgccccggcg cgccagaggg tggctgcggc
      841 tgagcgaatc aatacgccgc actgtctcca ggcgagtaga tagccaaaat tcttcgtctg
      901 catccagcaa tttgatcccg ccgatctcct tgaggtacag gcgaaccgga tcatcgctca
      961 attccatgat caatttcgga tcatccagga tgtcctgggg ctcttcttta accaaacttt
     1021 ccaattcttc tgcctccagg gtgagagcgg tatcgtctgg ctcttcaatt attgagtctt
     1081 cttggatctc ggcgagcaaa tcacccatct tatctagcga aagaggcgaa gtgatctccc
     1141 cattttctcg atcttcatga tgaggctggg ttcgatcttc cgacggtggt ttaggcgatt
     1201 tagaactttt taccattaca atttactcag cgatttacga tgacacggtc agaatactta
     1261 ccaagcgcct tattcaattg gttcaaaatt cggctattcg taaccacgat ctgcccatat
     1321 tctaccgcca tgagatcccc tgactgctgc gcctcttctt gaagaaagcg caagtggttt
     1381 atgaactgct ttagatggcg ctcgcgcagg tccaagatgg ctcgaaccag atctgcaagg
     1441 acgcgttcat catttggatc cagcttggcg gtgggtgcta taagcctatc ggcgacttcc
     1501 atcaagtcca gggagaggct attgagcaca tagttaagtg gctcagccac atcctgatcc
     1561 acggattcga gcaacaagtg gagtatcgcc tggtggtcgg catgctgaaa atcatcctca
     1621 gtgaggcggc ttagcccgct ctcctgcagg tggcgattga cttggtagag taaatctgga
     1681 cgacgtagca gcacacccag ggcgtgagct tcccgtcggt gggaaactga agtggtttta
     1741 gaagtgtccg gggtctcaac gggctcggcg gtcttgagct tttgaggcgc tcgtcgaggc
     1801 gggcgcaatc tggaactgcg cacctcaccc gagagggtgc gctcatctag tttgaggatg
     1861 cgggcaaggc gctggcggta ggtatcgcgc tcgatggcgc tgggcagatc gttgatcagt
     1921 ggtaacacct gggcagcaat ctggcttttt actttggggt catccaaatc ttgttgagca
     1981 gccaatgtct ccatgacgtg cagaacgacc ggcttagcat gctcgaggat acgctgccat
     2041 tcctgcgggt cacggttcac gacatcgtcg ggatccatgc cttcaggcag cgttgtaacc
     2101 cggatatccg cctgaaggcg agcctcaaag ccaagcagac cgcgggcatc aaagacgagc
     2161 tcctgctcac gatccagcga ttggcgagcg atttgtagcc cgcgcagcgt agcctgactg
     2221 cccgccgcgt cgggatctag cgccatgaca atgcggcggg tgtagcgctt gagcaagtac
     2281 aggtggcgct cggtaagagc agtgcccatc ggggaaatag cattgtcaaa gccagcctgg
     2341 tgcagggcga ttacatccag atagccttct acgatgacgg cttgatccac tttgcggatg
     2401 ggcttccgag cgcgatccaa tccataaagc agttggcctt tgtcaaagat agctgtttgg
     2461 ggggaattca ggaatttggg tatatcgttg gggtccaaga tacgcgcccc gaagccagcc
     2521 atgcgcccac gttcatcacg gatggggaac atgatgcggt ggcgaaagcg atcatatatc
     2581 ctggcgggat gctggcttcc cccttcagcc tcactctggc tcgactgggt gagcagacct
     2641 acggccagca aatcatcgtc cgtgtagttc ttggatttga aatagctcag cgttgcatcc
     2701 caagactgcg gggcatatcc caggtcgaag gcttcgattg tttcatcgtt taggccgcgc
     2761 tggtgcaggt aagccaacgc ctgctgacca gcaggcgtat taagcaattg gtgccgataa
     2821 aatgtgactg cctcttcgag caaaagacgc aagctatcgt gctcttccgc agcagcctgt
     2881 tcttctgggg tgactggctt aagttccacc ccagctttgt ctgccaggaa gcgtaatgtc
     2941 tccgcaaaat cccagccctc tttcttcatc acgtagctaa agatgtctcc cccttcattg
     3001 cactgaccga agcagcgcca ggtgcccgtt tcagggaaaa ctacaaatgc tggggtgcgc
     3061 gtattgctgt gaaatggaca aaaccctgta taattcttgc cagagcggcg cagttgcacc
     3121 gattccgaga caagatctat aatatctagg cgggctttta tctcgtcaat tgtactcatg
     3181 ggcttatacg attgagcatg taaattatac cagcaggaga atatttcatc ctgcgaatgg
     3241 actggtttat aatcagatga tgactacatc ccaaaactct cccgaaggtt atatctcggt
     3301 ggatgtcgag accgccgggc cgaatcccag ccaatattcg ctgctgacaa ttggcgcatg
     3361 caccttgagt gggagcccgc gcacgtttta cgtggagctc aagccggtca atatgaatgc
     3421 gatcccagag gcactggcag ccagccgtct ttccatggag cacctggctg agcgaggact
     3481 ggaacccgcc gaggcgatgc tccgcttcga ggactggcta aaggttgagg tacccagtgg
     3541 gcaaaagccg ttgttcgtct ccttcaatgc gccttttgat tggatgtttg ttaacgatta
     3601 tttttatcga ttcctcgggc gcaatccgtt tggacatact gcggtggata ttaaggccta
     3661 ttacatgggt ctgacgggtg tgaactggga tcagacatcc atgaaaatcc tcggtcctcg
     3721 ctacctggga aacagagaac ttacccatca cgccttgcgt gacgccatgg accaagccga
     3781 actcttccga
//
LOCUS       DBNHOH010000886         1498 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1723190, whole genome shotgun sequence.
ACCESSION   DBNHOH010000886 DBNHOH010000000
VERSION     DBNHOH010000886.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1723190"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..1417
                     /locus_tag="AC16V7_16000"
     CDS             113..1417
                     /locus_tag="AC16V7_16000"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HIR6258676.1"
                     /translation="MKAKKTSKFMAYLSVLTGGLLLLSGFAAAGGYLGLRLVQFFGAG
                     LLVDELGVTAAVFLGLVGGGLAVVHGFGSIINRRSRPLKLPPFYLFWIAFALVLGVGN
                     VVLLTGVSQEFLFPPLFLLGALLPTAAVLAWSFRALGWPVTWRQGALMFVAGSTLSIL
                     VTILLDSFLPYVFFLLVEPIGYLAEEFLSVFYSGGPEFFERLFFSPLLIFYLLYIAFQ
                     APFPEEFAKALGPRLMGVRITNERQAFAVGLASGAGFAILENMLYQGIYADWAGWTWG
                     GITALRGIGAVNHSLWTAIIALALYRERERKPGWFGRLGRAYLISVGLHTLWNGGYLA
                     LLYLVGLEQYAEVGATLPIYGEYVEISLIVILLALSLGMWWLLRKFVHQIARGAEAEL
                     TPELISRRALASWAFACVLVVVPLGAALGKAWGQIRAVFLGG"
BASE COUNT          223 a          412 c          460 g          403 t
ORIGIN      
        1 ttgctggcgg tctttcaaat tggggatgcg gcgtcccgta gcaaggcgct cgagcagttg
       61 aaatcgatgg gcgaggtgga agtgttttga cccgcttcga tggaggtgcc agatgaaggc
      121 gaaaaaaaca tcaaagttca tggcatacct ttctgtcctg accggtggtt tgctgctctt
      181 gagcggattt gccgccgcag ggggctacct gggactgcgc ctggtccaat ttttcggcgc
      241 aggtttgctc gtagatgagc ttggcgtgac cgccgccgta tttttgggcc tggtcggtgg
      301 aggcttggcg gttgtccacg gattcggctc aatcataaac cgccgctcaa gacccctgaa
      361 gctgcctcct ttctacctgt tttggatcgc ctttgccctg gtgttgggtg tgggtaatgt
      421 agtgctcttg accggcgtat ctcaggagtt cctcttcccc ccgcttttcc tgctgggagc
      481 gctgctgccc accgcagcgg tgctggcctg gtcctttagg gcgctgggtt ggccggtcac
      541 ctggcggcag ggcgccttga tgtttgtcgc cggttccacc ctgtccatcc tggtcaccat
      601 cttgctggat agctttctgc cgtatgtctt cttcttgctg gtggaaccta ttggttacct
      661 ggcagaggaa tttctcagtg tattctactc cggcggtcca gaattcttcg agcgcctgtt
      721 tttctcaccc ctgttgatct tctacctgct ctacatcgcc ttccaggccc catttccgga
      781 agagtttgcc aaggcgctcg ggccccgctt gatgggggtg cgcatcacca atgaacggca
      841 ggcctttgcc gtagggctgg cttccggagc tggattcgcg atcctggaga atatgctcta
      901 tcagggcatc tacgccgact gggcaggttg gacctgggga ggcatcaccg ccttgcgcgg
      961 tatcggagcc gtcaatcatt cgctgtggac ggccatcatt gcgctggcgc tgtatcgcga
     1021 gcgggagcgc aagccgggct ggtttggcag gctgggacgc gcctacctga tctcggtagg
     1081 tttgcacacc ctgtggaatg ggggctacct cgccctgttg tacctggtgg gtttggagca
     1141 atacgccgaa gtcggcgcca ccttgcccat ctatggagaa tatgtcgaaa tctctttgat
     1201 tgttatcctg ctggcacttt cacttgggat gtggtggctg ttgcgcaagt ttgtccacca
     1261 gatcgcccgc ggggcggagg ccgagcttac cccagagttg atctctcgcc gggcgctggc
     1321 ctcgtgggct ttcgcctgcg ttttggtggt cgtaccgctt ggagcggctt tgggtaaagc
     1381 ctgggggcag attcgggctg ttttcttggg tggataattt acccgcttgc atttcaccca
     1441 tttatcctat acaatgagac cagtatgctg ctcactgccg atgcgatgcg cgcttccc
//
LOCUS       DBNHOH010000887         6987 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_890013, whole genome shotgun sequence.
ACCESSION   DBNHOH010000887 DBNHOH010000000
VERSION     DBNHOH010000887.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6987)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6987)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6987
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_890013"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3611)
                     /gene="rpoC"
                     /locus_tag="AC16V7_16005"
     CDS             complement(<1..3611)
                     /gene="rpoC"
                     /locus_tag="AC16V7_16005"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIR6258677.1"
                     /translation="METKGLTSLRISLASPETIQSWSYGEVLKPETINYRRLRPEKDG
                     LFCEAIFGPTRDWQCYCGKYKNIRYKGIICDKCGVEVTRSSVRRERMGHIELSAPVAH
                     VWYTRRIPSYLGLLLDISRRNLDRVLYFAQYVVTYVDEDARQKALKRLEDKITVTERE
                     QALKINTRIAEVKGERDRTLAELNQRRSEIEKRFEDTIAEQLDPIIKGGQRLEASLRE
                     QMGQAPRTAIHFPDTDIVIVEAGKTINNRNLSSVQSVVKERLEELENELKDQKQRELD
                     SNKMEVERVRAQADLEMENLRMQLDDQASSVRDENARMRDELLELRPLTFMGESRFRE
                     LKQRWGQVFRADMGAEAFYDILRRLDLDKLAQELWVEVRTSRSKQKRKKATKRLKVVE
                     AFRRSANRPEWMVLTILPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLL
                     ELGAPDVIVRNEKRMLQEAVDSLIDNSQRGKALSRRGRRELKSLSDMLKGKKGRFRRN
                     LLGKRVDYSGRSVIVVGPKLKLYQCGLPKNMALELYRPFVISRLVTHNYAANVKGAKR
                     FIERNRPEVWEVLEEVIRERPVLLNRAPTLHRLGIQAFEPVLIEGSAIQLHPLVTTAF
                     NADFDGDQMAVHVPLSQKAVWEARQLMLSSKNLLKPADGEPIISPSKDMVLGVYYLTK
                     SDESVHNGDGHIFGNMDEVELAYSLNIVDIHSRIKLRTDTWFDDQNNRLPKAEDRIIE
                     TTVGRVIFNRVLPTQMQYVNWVLDKGGLKDLIADLYEICGEENTPDIADAIKDIGFTY
                     ATRSGYSLAVSDITVPLEKQSIIQDALQEQETVQRDFRRGLLTEQEQNERIIEIWQRT
                     TNSVADAVKRTMDPNGNLAAQAISGATKGGFGPISQLAGMRGLMADPSGRIIPLPIRS
                     NFREGLTALEYFISTHGARKGLADTALRTADAGYLTRRLVDVAQDVIINDDDCGTADG
                     IWIRRSDDIAGQSISVRLYGRLVAERVVDPETGEILAEYNDVLDHERVRRIANSNVQE
                     VKARSPLTCELIHGICTKCYGLDLGRGKLVEVGSAVGIVAAQSIGEPGTQLTLRTFHT
                     GGVAAGGDITTGLPRVEELFEARKMPKGEAVVTEIAGRASVMQSDRYSDLRMVRVEHS
                     EMVSNEYSIPEEWTIVAEDGSEVSQGDVLAFLGEDASITTEHAGRVRLEDRNVIVSY"
     gene            complement(3908..5113)
                     /locus_tag="AC16V7_16010"
     CDS             complement(3908..5113)
                     /locus_tag="AC16V7_16010"
                     /inference="COORDINATES: protein motif:HMM:NF033564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HIR6258678.1"
                     /translation="MDYINLAPQEDVNENGMIFDVDSLYAYFQRVSDNRKAKGKQYPL
                     VQLLFLMMLSKLGGEDKPSGMADWVSNRLEQLFEMRVLVKKKAPSHMTYRRVLQGTIQ
                     PEEFERLISEYQQAHMEKGEEIVFSMDGKSLKGTIARGAVRGTHLLSIYVPEQGLVLV
                     EAAVDRKENEIVVAPKILKQVNLSGAIVIGDAMHAQREASAQIVESGGDYLWSIKGNQ
                     ARTEWAIQKLFVHEVCNLKQGAPLSKHCQMVSQVHKGHGRIEKRTIMVSTELNEYLDW
                     PYVAQVFRLEREVWHEKYRGRTRQVVYGLTSLTPLQASPRKLLALTRQYWGIESGLHY
                     RRDVTLHEDSTRLTVSAAGHNMAILNNLLIGLCIRNGFTNIAKARRMLCAHPKYALAM
                     IVSSNNPFL"
     gene            complement(5521..>6987)
                     /gene="tnpC"
                     /locus_tag="AC16V7_16015"
     CDS             complement(5521..>6987)
                     /gene="tnpC"
                     /locus_tag="AC16V7_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HIR6258679.1"
                     /translation="PSQDEVRAAYQQGEEAVVVLINRLIEIIAILAAEVQALRNQLAK
                     NSRNSSKPPSSDGLKKKPTKRGLRKPSGKKSGGQPGHEGHTLKAVVHPDRVEVHRVER
                     CKRCQAELSLVEAYGVEKRQVFDLPVVRLEVTEHQAEIKTCPQCMQVNKAEFPLGVTQ
                     PVQYGVEIKAQAVYFNQYHHIPLERTCEILEELYDSAFSEGTLVESCEQVAEAVRPVG
                     EQAKEYLIQTEEVVHLDETGGRVEGKLRWVHVSSTETLTYLEMHEKRGRLAHDGMGIL
                     PERTGWVMHDGYHSYKQYPNAQHALCNAHHLRELLFIHERYQQPWAGAMLQLLLEIKQ
                     AVECAQQNQLAALFPEQIAHFESRFDELIGQGLKANPPPDPPVPRKRGRVKQSPAKNL
                     VDHLHQQQSAVLAFMCDFKVPFDNNLAERDLRMVKLKQKVSGCFRSVQGARTFCRIRS
                     YISTARKNCLQALDALRMAIRGDPFYPPCLLAPAASAT"
BASE COUNT         1550 a         2018 c         1737 g         1682 t
ORIGIN      
        1 tcatacgaga cgatcacatt ccggtcttcc aggcggacgc gaccggcatg ctcagtagtg
       61 atcgaggcgt cttcaccaag gaatgcaagc acatcacctt ggctgacctc cgatccatct
      121 tcggcaacga tggtccattc ctcagggatg ctatactcat tacttaccat ctcagagtgt
      181 tccacccgca ccatgcgcaa atcgctatat cggtccgatt gcatcacaga agcgcgacca
      241 gcgatctcgg tgacaaccgc ttcgcccttg ggcatcttgc gggcttcgaa gagctcttca
      301 acacggggta gacccgtcgt aatgtcacca cccgccgcga cgccgccagt atggaaagtt
      361 cgcagggtga gttgcgtgcc cggctcgccg atggattgag cggctacaat gccgaccgcc
      421 gaacccacct ccaccagttt accgcgtccc aaatcaaggc cgtagcactt cgtacatatg
      481 ccgtgaatca actcgcaagt cagcggtgat cgagctttta cttcctgcac attggaattg
      541 gcaatacgac gcacgcgctc atgatccagc acatcgttat actcggccaa tatctcgcca
      601 gtctctggat cgactacgcg ctctgccacc aatcggccat atagccggac agatatcgat
      661 tgaccggcaa tatcatcact gcgcctgatc cagattccat ccgctgtgcc acaatcatca
      721 tcattaatga tgacatcctg agccacatcc acaagacgcc gcgtgagata acctgcgtca
      781 gcggtgcgca gggccgtgtc agcaagaccc ttgcgtgcac cgtgggtaga aataaagtat
      841 tccagcgcgg tcaagccttc gcggaagttc gaacggatgg gcagggggat gatacgtccg
      901 gaaggatcag ccatcaggcc gcgcatacca gctaactggg aaataggccc aaagccacct
      961 ttggttgctc cagagatagc ctgcgctgcc aagttgccgt taggatccat tgtgcgcttt
     1021 acagcatcgg caacggagtt ggtcgtgcgc tgccagatct cgatgatgcg ctcattttgc
     1081 tcctgctcag tgagcaaacc acgccggaag tcgcgctgca cggtttcttg ctcctggagc
     1141 gcatcctgaa tgatgctctg tttctccaac ggcaccgtga tatcagacac ggccagggag
     1201 tagcccgacc gggtagcata agtaaagccg atatctttga tggcatccgc gatatcaggt
     1261 gtgttctctt caccacagat ttcatacagg tcagctatta gatctttcaa cccacccttg
     1321 tccagcaccc agtttacgta ctgcatctgg gtcggtagga cacggttgaa gatcacgcgc
     1381 ccaaccgttg tctcgatgat gcgatcttcc gctttcggca agcggttgtt ttgatcatca
     1441 aaccaggtat ctgtcctaag cttgatacga gaatggatat cgactatatt caaactgtag
     1501 gccagttcta cttcgtccat gttaccgaaa atatgcccat ccccattatg cacgctttca
     1561 tcactcttgg tcagataata aacacccaag accatatcct tggatgggct gatgatcggc
     1621 tcaccatcgg ccggtttcag caggttcttg gaagagagca ttagctggcg tgcctcccag
     1681 acagctttct gggagagcgg gacgtgaacc gccatttggt caccgtcaaa gtcggcattg
     1741 aaagcagtgg taaccaatgg gtgcaactgg attgcgctac cttcgatgag caccggctca
     1801 aacgcctgga tgcccaagcg gtgcagcgtg ggagcccggt taagtaaaac tggtcgttcc
     1861 cggatgacct cttctagcac ttcccaaact tcaggacggt tgcgctcgat gaagcgcttg
     1921 gcgcctttta cattggcggc gtagttatgc gtcaccaggc gggagattac aaagggacgg
     1981 tacagctcaa gggccatgtt cttgggcaag ccgcattgat acagcttaag cttcggacca
     2041 accacaatca ccgaacgccc cgagtagtcc acgcgcttac ccaaaaggtt gcggcggaag
     2101 cgaccctttt tgcctttgag catatcgctg agtgatttca actcgcggcg gccacggcga
     2161 gagagcgctt taccgcgctg cgaattatcg atgagcgagt caactgcctc ttgcaacatg
     2221 cgcttttcgt tgcgcacgat gacatctgga gcacccaatt ccagcaagcg cttgaggcgg
     2281 ttattgcgat tgataacccg tcggtacagg tcgttcaggt cagaggtggc aaaacgccca
     2341 ccgtctaact gcaccatagg acgtagatca ggcgggataa ccggcaaaat ggtcaaaacc
     2401 atccactccg gtcggttagc agaacggcgg aaagcctcaa caaccttaag acgtttggtc
     2461 gcttttttgc gcttttgctt gctgcgcgat gtcctcactt ccacccataa ttcctgagcc
     2521 agcttatcca gatccaggcg gcgcaagatg tcatagaaag cttctgcacc catgtcggcg
     2581 cgaaaaacct gtccccagcg ttgcttaagc tcacggaagc ggctctcacc cataaaggtg
     2641 agcgggcgca attccagcag ctcgtctcgc atgcgggcat tttcatcgcg tactgaagat
     2701 gcctgatcgt ccaattgcat gcgcaggttc tccatctcaa gatcagcctg cgcgcgcacg
     2761 cgctcgactt ccatcttgtt gctatcaagc tcgcgctgct tctgatcctt cagctcattc
     2821 tcaagctctt ccaggcgttc cttgaccacg ctctgcacgc tggacagatt ccggttattg
     2881 attgtcttgc cagcttcaac gatgacgata tcggtgtcgg ggaaatgtat ggctgtgcga
     2941 ggtgcctgac ccatttgctc acgtagagaa gcctcaaggc gctgcccacc tttgatgata
     3001 gggtcgagct gctcagcgat cgtatcttca aatcgctttt caatctcgga cctgcgctga
     3061 ttcaactctg ccagagttcg atcacgctct cccttgactt ctgcaatgcg ggtgttgatt
     3121 tttaacgctt gctcgcgctc agtgacagta atcttgtcct ccaagcgctt gagagctttc
     3181 tggcgtgcat cttcatcaac gtaggtcacc acatattgag cgaaatacaa cacccggtct
     3241 agattgcgcc gggaaatatc cagcaacaaa cctaaatacg acggaatgcg gcgtgtgtac
     3301 caaacgtgcg ctacaggggc agataactcg atatgcccca tgcgctcgcg acgcactgaa
     3361 gagcgagtga cttctacccc gcatttatca cagataatac ctttataacg aatattctta
     3421 tatttaccgc aatagcactg ccaatcccgt gtgggaccaa agatcgcctc acagaagagg
     3481 ccatctttct ctgggcgcaa tcggcggtag ttgatggttt ctggctttaa gacctctcca
     3541 taagaccaac tctggatagt ctctggcgaa gccaggctga tacgaagact ggtcaaccct
     3601 ttagtttcca ctcggctgcc tcctacatct aatctctaat ccaccactaa cctaaaaaga
     3661 agacacactt ttaccatcca cagcggtttc gctgccgctg ttgaaaccgt ttttgagtgg
     3721 cgtcttagca tcaattggtt cggggatcgt aatatttaaa agcttaatac aaacaaagac
     3781 aagcgcattg agcacctccg tctcaagcgc ttatcacgcg cgctacctca ttgatttatc
     3841 atctgaatac attataccaa ccatgatcac ggttggtcaa gaggaaaatg tgatcgttcc
     3901 cagggcttca caagaaagga ttattggacg aaacgatcat cgcaagagca tactttgggt
     3961 gggcacaaag catacggcgg gctttagcga tattggtgaa gccattacgg atgcacagtc
     4021 caatcagcag gttattgagg atagccatgt tgtggcctgc ggcactgacg gtaagccggg
     4081 tactatcttc atggagggtg acatctcgcc gataatgcag tccactctcg atgccccaat
     4141 actggcgggt caaagccagc aactttcttg gtgatgcttg caggggtgtc aggctggtca
     4201 agccatacac cacctggcga gtgcggcctc gatacttttc atgccacact tcacgctcca
     4261 ggcgaaagac ctgggcgaca tacggccaat cgagatactc attcaactcg gtactcacca
     4321 tgatcgtgcg cttttcaatc cggccatgcc ctttatggac ctgagaaacc atctggcagt
     4381 gtttcgagag gggggcaccc tgcttgagat tgcagacttc atgcacaaac agcttttgga
     4441 tcgcccactc ggtgcgagcc tgattgcctt tgatgctcca aaggtagtcg cctccgctct
     4501 caacgatctg agcggaagcc tcccgttggg cgtgcatagc gtctccaatt acaattgccc
     4561 cgctcaagtt gacttgtttg aggatcttgg gggcaaccac aatctcattt tccttgcgat
     4621 ccactgccgc ttctaccagt accagccctt gttccggcac ataaatgctc aacaggtggg
     4681 tgcctcgcac tgcccctcgt gcaatcgtcc ccttcaggct cttcccgtcc atgctgaaca
     4741 cgatctcttc ccctttttcc atatgcgcct gttgatactc gctgatcaaa cgctcgaact
     4801 cttccggctg tatcgtacct tgcaacaccc ggcgataggt catatggctg ggcgccttct
     4861 tcttgaccaa taccctcatc tcaaaaagct gctcgagacg gttgctcacc caatcggcca
     4921 tcccactcgg tttgtcctcg cctcccagct tggaaagcat catcaaaaac agcagctgta
     4981 ccaggggata ttgcttgccc ttagccttgc ggttgtcact cactcgttga aaatatgcat
     5041 ataagctgtc gacatcgaat atcatcccgt tctcatttac atcttcttgg ggtgcaagat
     5101 tgatataatc catgttggtc ttctccggct acttgtgttt tggtcaacaa agtataccga
     5161 agaagacctt cttttttgtc aagctacttt gtgaaagccc tggtggcggt gtctgtccca
     5221 aaattggtgg ggaaaatcaa agagggcagg aagacaggca tagcccgatg ttgcttaatc
     5281 tctcaaccca accccgatcc agccgccatc aatccagcga gcaaccccgc ctgagttatg
     5341 taaccgcgca ccgtgcggct acgtatcttc atgcggctta ctctatgcca ttattggtca
     5401 agggtatacc atttatagtg aaaaaatacc cccgtccggt agtcacgtga ttatacaagg
     5461 ggctagcgcg accctaaagt gaacatgggg ggtgcacaac tgaatatttt tcgtaactgc
     5521 tcaggttgct gacgcagccg gggccaatag acaaggagga tagaacgggt caccacggat
     5581 cgccatgcgc agcgcatcca acgcttgcag gcaattcttg cgagccgtcg agatataact
     5641 gcgaatacgg cagaaggttc tggcaccctg taccgagcga aagcaacccg ataccttttg
     5701 tttcaacttg accatgcgca aatcgcgctc ggccagattg ttgtcaaatg gcaccttaaa
     5761 atcgcacata aaagccagca cagcggattg ctgttggtgc aggtgatcaa ccaggttctt
     5821 ggctgggctt tgtttgaccc ggccgcgctt tctgggcaca ggcgggtctg gaggcggatt
     5881 ggccttcaac ccctgcccga tcagttcgtc aaaacggctc tcgaagtggg cgatctgttc
     5941 tgggaagagt gcggcaagct ggttctgttg ggcgcattca accgcctgct tgatctccag
     6001 gagcagttgg agcattgccc cggcccaggg ctgctggtag cgctcgtgaa tgaagagcag
     6061 ctcgcgcagg tggtgggcgt tgcacagggc gtgctgggca ttgggatact gcttgtagga
     6121 gtgatagcca tcatgcatca cccagcctgt acgctccggc aagataccca tcccatcatg
     6181 agctagcctg ccacgctttt catgcatttc cagataggtg agcgtctcgg tactggacac
     6241 gtgcacccag cgcagcttgc cttctacccg cccgccggtt tcgtccagat gtaccacctc
     6301 ttcggtctgg atcagatact ccttggcttg ctcaccgacc ggcctgaccg cctccgccac
     6361 ctgctcgcag ctctcgacca gcgtgccttc actgaatgcg ctgtcgtaca gttcttccag
     6421 gatctcgcag gtgcgttcca gcggaatgtg gtggtactgg ttgaagtaca ctgcctgggc
     6481 tttgatctcc accccgtact gcaccggctg ggtcacccca agtggaaact cggctttgtt
     6541 cacctgcatg cactgcggac aggtcttgat ctcggcctga tgctcggtca cttccagccg
     6601 caccaccggc agatcgaaca cctggcgctt ctccacccca taggcttcta caagcgaaag
     6661 ctccgcctgg cagcgcttgc agcgctccac ccgatgcacc tctacccgat ccggatggac
     6721 tactgccttc aaggtgtgcc cttcatgccc tggctgtccg ccgctcttct ttccgctcgg
     6781 cttgcgcagc ccacgcttgg tcggtttctt cttcaatccg tcgctcgacg gtggcttgct
     6841 gctgttacgg ctgttcttcg ccagttggtt tctcaatgcc tgtacttcgg cagctaatat
     6901 ggcgatgatt tcgatcagcc gattgatgag cacaacaacg gcctcttcac cctgctggta
     6961 tgctgcacgc acttcatcct gagatgg
//
LOCUS       DBNHOH010000888         2476 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_505672, whole genome shotgun sequence.
ACCESSION   DBNHOH010000888 DBNHOH010000000
VERSION     DBNHOH010000888.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_505672"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /gene="gnd"
                     /locus_tag="AC16V7_16020"
     CDS             complement(<1..940)
                     /gene="gnd"
                     /locus_tag="AC16V7_16020"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HIR6258680.1"
                     /translation="MGQNLVLNMNDHGYRIAVYNRTISKVDEFLETSAKGTEVVGAHS
                     LEELVSLLKHPRRVMLMIKAGQPVDDFIDLLIPLLEPGDIIIDGGNSNFQDSIRRTAY
                     LESKGLLFIGTGISGGEEGARYGPSIMPGGSTQAWPHVKDIFQSIAAKVEDGSPCCDW
                     VGENGAGHFVKMVHNGIEYGDMQLIAEAYHLMKSGLGLSYDELHQVFAEWNRGKLDSY
                     LIEISRDIMAFKDEDGTPLVEKILDTAGQKGTGRWTVVEALENGVPLTLVAEAVLARS
                     LSALKEERQVASKKLKGPRKTFSASRERAIDDIRHAL"
     gene            complement(1103..>2476)
                     /locus_tag="AC16V7_16025"
     CDS             complement(1103..>2476)
                     /locus_tag="AC16V7_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR6258681.1"
                     /translation="RKTGEYVVSPPPEFSEEMIKQGDSGVDVGGENYIPRAEIRSPLP
                     VSSNVTLNESYTPAGRKARRSSVWTTLVLVVITLGLAGWAAGNFFPSWPSRLLSLINP
                     PSDNPWVVVVGDPTSIQTNEISTSTHTPTGTSSPTLEPSPTTTPTSTLEPTPTDLPVQ
                     PSETITQMPTPVGGGNGQIAYASDRSGTTQIWLVNVDGSSDPVQVTNMPEGACQPDWS
                     PDGLRLVFISPCNQNEDLYPGAGLFVIHADGSGLTPLPNVPGGDFDPTWSPDGNLIAF
                     TSLRNSGRPRIYVLDLRDNSVTRLSGQYSYDRQPAWSPDGSRIAFVTTQKGPVQIWVM
                     DSNGSNQAIYTRSADSINTHPVWSRDSDLILFNQVEPGGGPPYIVAVSYNDGQYNEFR
                     YQLGPWPVREAQYSPDGLWLVFESWPQGSNHDIYVMSASGAGRTQLTSWERFDFDPAW
                     RPMVQAP"
BASE COUNT          613 a          674 c          596 g          593 t
ORIGIN      
        1 ataatgcatg gcggatatcg tcaatggctc tctctctcga ggccgagaag gtttttctcg
       61 gtcccttgag cttcttggag gcgacttgcc tctcttcctt gagcgcggac aatgaacgtg
      121 ccagcactgc ttccgcaacc aaggtcagag gtacgccatt ctccaaagcc tcaaccactg
      181 tccaacgacc agttcccttc tgaccggccg tatccaggat tttttcaact agaggagtcc
      241 catcctcgtc tttgaatgcc atgatatcgc gtgagatttc gatcaggtag gagtctaatt
      301 ttcctcgatt ccactcagca aatacttggt gcagttcatc atagcttaaa cccaaccctg
      361 acttcatcaa gtggtacgcc tctgcaatca attgcatatc gccatactcg ataccattgt
      421 gcaccatttt gacaaagtga cccgccccgt tttcacctac ccaatcacag caaggtgagc
      481 catcctcaac tttagcggcg atgctttgga aaatgtcctt aacgtgtggc caggcttggg
      541 tagaccctcc tggcatgata gacgggccat agcgagcgcc ctcctctccc ccagagatgc
      601 ccgtaccgat aaaaagcaaa ccctttgact caaggtaggc tgttctgcgg atcgaatcct
      661 gaaaattgga gttaccacca tcgatgataa tatcgcccgg ctctagcaat gggatcagca
      721 aatcaataaa atcgtccact ggttgtccag ccttgatcat gagcattacg cggcggggat
      781 gcttgagcag cgacacaagc tcctccaaag aatgagcccc taccacttcg gttccttttg
      841 cactggtttc gagaaattca tctaccttag atatcgtgcg gttataaacc gcgatgcggt
      901 agccatgatc attcatattt aataccagat tctggcccat gaccgccaaa ccgatcaaac
      961 caagatcgca ttcaggttct gcgttgcttt tcactaggaa attccttttt acattgtgga
     1021 aaagtatgta ccgccagttt tgagaatact cacctcaaaa ggggtcgcga ggggtatttt
     1081 gagaaattta cattatttct cgtcagggtg cttgcaccat tggacgccag gctggatcaa
     1141 agtcgaagcg ttcccagcta gtcaattgcg tcctgccagc cccacttgca gacataacat
     1201 agatatcatg gttgctaccc tgcggccagc tttcaaatac gagccacagt ccatccggtg
     1261 agtattgagc ctcgcgtaca ggccaaggtc ccagctgata ccggaattca ttgtactgcc
     1321 catcattata ggataccgcc acgatatacg gtggtccccc tccaggctca acctgattaa
     1381 acaggataag atcgctgtcc cttgaccata ccggatgggt gttgatggaa tcagcgctgc
     1441 gcgtgtaaat agcttggttg ctgccattgg aatccataac ccagatctgc accggacctt
     1501 tctgggtggt cacaaatgca atcctgctgc catcaggcga ccaggcaggt tgccgatcat
     1561 aggaatactg cccggaaagg cgcgtgactg aattatcccg caggtctaat acatagatgc
     1621 gcgggcgccc gctgttgcgc aacgaggtga aagcaatcaa attgccatcc ggtgaccagg
     1681 tcggatcgaa gtcccctccg ggcacgttag gcaagggggt caagccagag ccgtctgcat
     1741 ggatgacaaa caagcccgcg cctgggtaca aatcctcatt ctggttgcat ggcgaaataa
     1801 ataccaggcg taatccatca ggcgaccagt caggctggca agccccttcg ggcatattcg
     1861 taacttgcac tggatcacta ctaccatcca cattgacaag ccaaatctgg gtggttcccg
     1921 aacgatctga tgcatacgca atctggccat tgccaccgcc aaccggggta ggcatctgag
     1981 ttattgtttc agacggttgg actggcaaat cagtgggtgt aggttccagt gtcgatgttg
     2041 gcgtggtcgt ggggctaggc tctaatgtgg gtgaagatgt gcctgtagga gtatgagtag
     2101 atgtcgatat ctcattagtc tgtatgctgg tcggatcacc cacgacaacg acccacggat
     2161 tatcgctagg aggattaatt agggagagca agcgtgatgg ccatgaaggg aaaaaattcc
     2221 ccgctgccca acccgccaaa ccaagcgtga taacaaccaa aaccaacgtc gtccaaacgg
     2281 atgaacgccg cgccttgcga ccagccggcg tgtatgattc attcaaagta acattgctgg
     2341 aaaccggcaa cggtgagcga atctctgctc gcggaatata gttctccccc ccaacatcca
     2401 ctcctgaatc cccttgttta atcatctctt ccgagaattc tggcggaggg gacacaacat
     2461 attcgcccgt cttgcg
//
LOCUS       DBNHOH010000889         1591 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1146266, whole genome shotgun sequence.
ACCESSION   DBNHOH010000889 DBNHOH010000000
VERSION     DBNHOH010000889.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1146266"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..>1591
                     /locus_tag="AC16V7_16030"
     CDS             23..>1591
                     /locus_tag="AC16V7_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258682.1"
                     /translation="MKRLKPDIKLLRSNFIKDLAIILLLALLTFTLGDTFLRLAGRGG
                     FPAMEIRISPYKSLSYALQYPDSQVSYRRWNAYSRVDLVRSSGIRSLPGLSYRYLQPP
                     PPQDGLLVDGDDPSPVVINLENLGFAEYLPASLAFHLRPDAQTLILEPRGGVDILTAL
                     AGGARQITAVEVNPLIVEAAGQIYASPRVQTVIDSGRSYLRRTDQQFDVIILSLISSY
                     HPVRSGAYSLAEDYRYTQEAFQDMLDHLKPGGLLVLMRWLQTPPSESLRVFALASTAL
                     EDRDADPKSQIVALRGYNAATLLIQNGPFTNAEIDTVREFAASRAFDLDYFPGIAPQD
                     VNQYNVLPEALDYQAYTDLLDSPTPQTFYRNYPFDISPPTDDHPFFSHSFKWEQADEV
                     IAEMGKTWQPFGGAGYFVVIALLLLALIGSSLLIVLPLAIHRRNMPLTEKSSGLSKVR
                     LRSLLYFGLIGIGFLFVEIPLIQKFILFLGNPAYAMTTVLFTLLLFSGLGSQFSHRIP
                     IRAGIGALVILVVIL"
BASE COUNT          354 a          430 c          377 g          430 t
ORIGIN      
        1 acctgctcac tggggtgatg tggtgaagag gctaaaacca gacatcaagc ttcttcggag
       61 taattttatt aaagacctgg caattatttt gcttctcgcc cttctgacct tcacattggg
      121 cgataccttc ttgaggctgg caggcagggg gggatttccc gcaatggaga ttcgcatttc
      181 cccctataaa agcctctctt atgccctgca atatcccgat tctcaggtca gctaccgccg
      241 ctggaatgca tactcgcgtg tggacctagt tcgcagcagc ggtattcgct ccctgcctgg
      301 cttgagctat cgctacctcc aaccccctcc cccacaagat ggtttgttgg ttgatggtga
      361 tgatcccagt cccgtggtga tcaatctcga aaatctagga ttcgctgaat atctgccggc
      421 gtccctggcg tttcacctgc gtccggatgc acaaaccctc attttggagc cacgcggtgg
      481 tgtggatatc ctgaccgccc tggctggcgg ggcgcggcag attaccgccg ttgaggtcaa
      541 tccgctgatt gtagaagcag ctggtcaaat ttatgcgtct ccaagagtac aaactgtcat
      601 tgattccggg cgcagctacc ttcgtagaac agaccagcaa ttcgatgtca tcattctctc
      661 cctgattagc tcgtaccatc cggttcgctc cggtgcgtac agcctggctg aagactatcg
      721 ctatacacaa gaggccttcc aggatatgct ggaccacctg aagccaggtg gcttgctcgt
      781 gctcatgcgt tggctgcaaa ctcctcctag tgaaagcctt agggtcttcg ctctagcatc
      841 cactgccctg gaagatcgtg atgcagaccc gaaaagccaa atcgtagcct tgcggggtta
      901 taacgcggct accctgttaa ttcaaaatgg tccatttaca aacgccgaga tagataccgt
      961 gcgtgaattc gccgcctcgc gcgccttcga cctggactat ttcccaggga tcgcaccaca
     1021 agatgtaaat caatataatg tgcttcccga agcgttggat tatcaggcat acacggacct
     1081 attggactcc cccactccac aaacttttta ccgcaattac cctttcgaca tcagtccccc
     1141 gacagacgat catcccttct tcagtcattc ctttaaatgg gagcaggcag acgaggtaat
     1201 tgctgaaatg ggaaaaacct ggcagccttt tggcggggca ggatattttg tggttattgc
     1261 cttgttattg ttagctttaa tcggctccag cttgttaatc gtcttaccgt tagcgattca
     1321 ccggcgaaac atgccactca cagaaaaatc aagtggatta tcaaaggtta ggcttcgatc
     1381 attactatat tttgggttga tcggtatcgg cttcttgttt gtggaaatcc cgctgatcca
     1441 aaagtttatc ctgttcttag gcaacccggc atacgccatg accactgtcc tcttcacatt
     1501 attattgttc tctggcttag gtagtcaatt cagccacaga atcccgatcc gggcaggaat
     1561 aggtgcgctg gtgatcttgg tcgtcattct t
//
LOCUS       DBNHOH010000890         1959 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1723688, whole genome shotgun sequence.
ACCESSION   DBNHOH010000890 DBNHOH010000000
VERSION     DBNHOH010000890.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1723688"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            487..1371
                     /locus_tag="AC16V7_16035"
     CDS             487..1371
                     /locus_tag="AC16V7_16035"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6258683.1"
                     /translation="MDIGFVGLGVMGEGIVPRLLAAGHSVTGWNRTQGKAEPLIKLGM
                     HWADSPREVAKQSEIVFSIVTDAAAVRAVALGTDGIIAGLREDGIYLDMSTIAPDASR
                     AIAAEFESAGLTMLDAPISGSPITLAQGKASIMVGGNKDAFERVHPVLLDIGAKAMYI
                     GGNGLAVQMKLAINLVLMVEVIAFGEGIALAEKGGVKREVAVEAMLNSVAASPVLGYR
                     GPFILEGNMPEVPLADVTLQQKDMILVLDLGRKLGSPVPLAAAANEMMNACRGLGIDH
                     RDFVTAHEVYRVLGGMLK"
     gene            1368..1784
                     /locus_tag="AC16V7_16040"
     CDS             1368..1784
                     /locus_tag="AC16V7_16040"
                     /inference="COORDINATES: protein motif:HMM:NF012417.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6258684.1"
                     /translation="MKPYRTNLRDVPYVEGLSPRDGWINMQVQFLIDEKTANSDKLVL
                     GWTVLPPGARHDRHRHFQADEFLLVLKGHGEIYTDEGNEPAGEGDVVYTTRGHWHGFN
                     NTSDEEVVLVWGWSGAGSLESAGYEVSHDHSHSDQA"
BASE COUNT          452 a          450 c          566 g          491 t
ORIGIN      
        1 aattgatgaa gagcggggcc gcaaccccat atattgcgct ttataagtct atagaatcaa
       61 taatgggcat tgattctatg caaattgcac cacaattgca ccaacaagga aaggcgtttt
      121 gtaacccggt ccttaatatt tactgtttct ttttctaaaa atgctatttc gccaggtgtc
      181 gtaacgaacc ccattggcgt aggatcagca cgcataggct cgatcacagt tgccgcagtc
      241 tggatattcc tgctggcctt aatcatcttt taccgcgagg aggttaacct gcgctggccc
      301 gatcttccag acaagccaaa tcttcttcaa agttttacct tacccaactg atcccattct
      361 gaagcaagca tggggattca gatcatcaag catggcaatt gggaatcccc atagctgagg
      421 atgttgacta catcaagata catccttata atctctaaaa ttttatgaga ccctaggagg
      481 tgcgcattgg atattggctt tgttggattg ggtgtgatgg gggaaggtat tgtaccgcgc
      541 cttctggcag ctggacactc tgtcacaggt tggaatcgga ctcaggggaa agcggaacct
      601 ctgatcaaac tggggatgca ttgggctgat agtccgcgcg aagtggcaaa acaatcggag
      661 attgtgtttt ccattgtcac agatgcagcg gctgtcagag ccgtggcgct tgggaccgat
      721 gggattatcg ccgggcttcg ggaagacggg atatatttgg acatgagcac gatcgccccg
      781 gatgcaagcc gtgcaattgc cgctgaattc gaatcggcgg gcttgaccat gttggatgcg
      841 cctatctctg ggagtcccat tacgcttgca cagggtaagg cctcgatcat ggtgggcggc
      901 aataaggacg cctttgagcg agtccacccg gtgttattgg atatcggagc gaaagccatg
      961 tatattggtg ggaatgggct ggcggtccag atgaagctcg ccatcaatct cgtgcttatg
     1021 gtcgaggtga ttgcctttgg cgaaggcatc gcgctcgccg aaaagggggg cgtaaagcgt
     1081 gaagttgccg ttgaggcgat gctcaacagc gtagccgctt ccccggttct aggttaccgc
     1141 ggcccgttta ttctggaagg gaacatgccc gaagtcccgc tggcagatgt taccttacag
     1201 caaaaggata tgatattggt cttggatttg gggcgcaagc tgggttctcc ggtgccgctt
     1261 gcagcggctg ccaatgaaat gatgaatgcc tgccgaggcc ttggcattga ccaccgagat
     1321 tttgtcaccg cccatgaagt gtatcgggtt ttaggaggaa tgttaaaatg aagccttatc
     1381 gtaccaattt gcgcgatgta ccctatgtcg aagggttatc cccccgggat ggctggatca
     1441 atatgcaggt tcaatttctg atcgatgaga agacagctaa ttcggataaa ctggtcctcg
     1501 gctggacagt cttgccgcct ggggcgcggc acgatcgtca tcgtcatttc caggctgatg
     1561 aattcttgct ggtgctcaaa ggtcatggag agatctatac cgatgagggc aatgagccgg
     1621 cgggcgaggg agatgttgtg tatacaaccc gcggtcactg gcatggtttc aacaatacct
     1681 ccgatgagga ggttgtcctg gtatggggtt ggagtggagc cggctccctg gaatcggctg
     1741 gctacgaagt ctcgcacgat cattcgcatt ccgatcaggc ataaacggtg aatgtagctg
     1801 gttacgattc gcgcatcctg cgcgggatgg agaaactcct aaagctacgc cagggtcgat
     1861 tggacgctgg tgagaaatcc atcgggtgga aagttggatt tggagcgcct gcctcattgg
     1921 aagccttaag acttgacgca cctctggtgg gctttctaa
//
LOCUS       DBNHOH010000891         2665 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_568958, whole genome shotgun sequence.
ACCESSION   DBNHOH010000891 DBNHOH010000000
VERSION     DBNHOH010000891.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_568958"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..410
                     /locus_tag="AC16V7_16045"
     CDS             156..410
                     /locus_tag="AC16V7_16045"
                     /inference="COORDINATES: protein motif:HMM:NF025807.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR6258685.1"
                     /translation="MRVTVHLHTILQRQTPEGLVRRLEVSLPDASTVGDLLSAMEITM
                     PLDSLLLAVNGRVADVDQVLKEGDQVNVMPAISGGSLNAR"
     gene            complement(462..1337)
                     /locus_tag="AC16V7_16050"
     CDS             complement(462..1337)
                     /locus_tag="AC16V7_16050"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR6258686.1"
                     /translation="MKSTQSLPENTHIGYTHLMVTDLERSLFFYQDILGFRIIEQERS
                     TVVLSASGEPPAQMILSTMIGVLPRPPHTTGLYHVAIRLPDRQALSRVFRRALERQIP
                     FHGAADHLVSEALYLPDPDDNGIELYVDRPRQEWPKLDGQIAMSTDPLDLRDLLNESE
                     NDGKVWDGIAAGTDIGHVHLQVSNLERAEAFYSDLLGLDVTQRGYPGALFLSAGGYHH
                     HLGLNIWTSRNAPPPPPEAVGLIAFKLNLPDENARQILLQRIQDTGQIIEEYSPSSLI
                     SGALIKDPDGIQIIL"
     gene            complement(1421..>2665)
                     /locus_tag="AC16V7_16055"
     CDS             complement(1421..>2665)
                     /locus_tag="AC16V7_16055"
                     /inference="COORDINATES: protein motif:HMM:NF046780.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 113"
                     /protein_id="HIR6258687.1"
                     /translation="SYRYCRDDQCGADDDTQTMGPASNGYPINSSLFQQTMKDQVDAW
                     AWLDSNPITVAVDPSDVTTVGSHFMAGIEMQPGYHPTWKPRAPEALTELQGLGANWVF
                     LTPTWTYTRNTPPILELASGRDSPWYDIQDNIQQARQRNLSVAIYPIAQLPSPTNQWW
                     AEAKRDFPWWSVWFERYRIFALHHADLAARNDAQALILGGDWLLPAMPNGTLTDGTPS
                     GVPADAETRWRNLIQEVRTHFGGVIIWAIPYSQAANNPPVFLDSVDRIYVHFNPQLST
                     VTNSGRPDLQAEAAKLLDTGVLPLQARFGKAIMIGVEYPSVDGAALGCLNGLPDGCLN
                     ASALARPNPDVSGFPIDLGEQADIYSGLLSAINGRSWITGFISRGYYLPVMLQDKSAS
                     VRGKPAQEVLKYWFPRLLGTIQ"
BASE COUNT          656 a          643 c          700 g          666 t
ORIGIN      
        1 ccctcaagct agagcgggaa ttcaacctgg gggctggttt tacctcagtt cacgaccgaa
       61 tccctgaatg gatgactcgc cagccgctac caccacacca agccgtattc gacgtgtctg
      121 agaaagaatt ggacgatttt ttcaactggt aagttttgag agttactgtt caccttcaca
      181 caatattaca aaggcagaca cccgagggtc ttgtgcgccg tctggaagta tcacttccag
      241 atgccagcac cgtgggtgac ttgctaagcg caatggagat tactatgcct ctcgattcgc
      301 tcttgctagc ggttaatggt cgcgtagctg atgtggatca agtgctgaag gaaggcgatc
      361 aggtgaatgt aatgccagca atttcagggg gctcgttaaa tgctcggtaa aagaatgagg
      421 ttcattcctg cgcagtctga tttatattaa tctcaatcga actataaaat gatttggatc
      481 ccgtccgggt cctttattag tgcacctgaa atcagactgc ttggcgaata ctcctcgata
      541 atttgtcctg tgtcctggat tcgctgcagt aaaatttggc gcgcattctc atcaggcaga
      601 ttcaacttga aggcgatcaa ccccactgct tcaggcggtg ggggaggagc attacggctg
      661 gtccatatat tcaaccctaa atgatgatgg tatccaccag cagatagaaa aagggcaccc
      721 ggataaccgc gctgggtgac atccaaaccg agcaaatctg aataaaatgc ttctgcccgc
      781 tccagattgg atacttgcag gtgaacgtgc ccgatatcgg ttccggcggc gatgccatcc
      841 cacactttac cgtcattttc tgattcattg agcaaatctc tcaaatccaa gggatctgta
      901 gacatagcaa tctgaccatc taacttgggc cattcttggc gagggcggtc tacgtatagc
      961 tcaatgccgt tatcatcagg atcgggtagg tagagcgcct cgctaactaa gtggtcagcg
     1021 gcaccatgaa aaggtatttg gcgctctagt gctcgtcgga acacacggct taatgcctgg
     1081 cgatcaggca ggcggatagc aacgtgatac aacccggtgg tatggggagg ccttgggaga
     1141 acaccgatca tggtggacag tatcatttgc gcaggtggtt ctccgcttgc agacaatacc
     1201 acggttgagc gttcctgctc gatgatacga aatccgagga tatcttggta aaagaagagt
     1261 gaccgctcta ggtcggtgac cattaggtgg gtatagccaa tgtgagtgtt ttcaggtaaa
     1321 gattgcgtac ttttcattgc tttttaatta gagcagttgg gtaggcgatg cccacccaac
     1381 tagtccatcg attgtgtata ctctataaac caaaaggaat ttattgtatt gtcccaagaa
     1441 ggcgtgggaa ccagtatttt aagacctctt gagcgggttt tccacgcact gaagcggatt
     1501 tgtcttgaag catcacaggt agatagtatc ctcggctgat aaatccggtg atccagctgc
     1561 gaccattgat ggcgctcaat aacccgctat agatgtctgc ttgttcgcct aaatcgatgg
     1621 gaaatcctga tacatcagga tttggtcggg caagagcgct agcgttcaag caaccatcag
     1681 gaagaccgtt cagacagccc aaagccgcgc catcaacgga cggatattct actccaatca
     1741 taattgcctt gccaaagcgc gcctgtagag gcaaaacccc agtgtccagc agcttggcag
     1801 cctcggcctg taaatccggt ctccctgaat tggtgacggt ggataattgt gggttgaaat
     1861 gtacgtagat acgatctaca gaatccaaga aaacaggtgg gttgtttgca gcttgtgaat
     1921 aaggtatcgc ccatatgatg acgccaccga agtgtgtacg gacttcctga atgagattgc
     1981 gccagcgagt ttctgcatct gccggcactc ctgatggtgt tccgtctgtt aatgtgccgt
     2041 taggcatagc gggcagtagc cagtctccgc ctaaaatcag ggcttgtgca tcgttgcgcg
     2101 ctgccaggtc ggcgtgatgc agagcaaaga tgcggtaacg ctcaaaccat acagaccacc
     2161 acggaaaatc tcgtttggcc tctgcccacc attgattcgt tggtgagggc aactgtgcga
     2221 tggggtaaat tgcaacgctc aggttgcgct ggcgcgcttg ctgaatgtta tcctgaatat
     2281 cataccaggg agagtcgcgc ccacttgcta attccagaat aggtggcgta ttgcgtgtat
     2341 aagtccaggt tggggtcagg aaaacccaat tggcgccgag cccttgtaat tcagtcagcg
     2401 cttcaggagc gcggggtttc cacgtaggat gatagccagg ctgcatctct attccagcca
     2461 taaaatgaga gccgaccgtt gtcacatcag atgggtcaac cgcaacggtg atcgggtttg
     2521 aatccagcca cgcccaggca tccacttgat ccttcatggt ttgttgaaac aagctggaat
     2581 tgatcggata cccgttactt gccgggccca ttgtttgggt atcatcatct gccccacact
     2641 ggtcgtcccg gcagtagcgg taact
//
LOCUS       DBNHOH010000892         1960 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_249318, whole genome shotgun sequence.
ACCESSION   DBNHOH010000892 DBNHOH010000000
VERSION     DBNHOH010000892.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_249318"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..594)
                     /locus_tag="AC16V7_16060"
     CDS             complement(82..594)
                     /locus_tag="AC16V7_16060"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR6258688.1"
                     /translation="MDEHLQSAAQAIQERFGANISEYRGDVSLLVSPDQIVEVCRALR
                     DEFNFEMLSGETAVDYWPELEPRFHLVYLLYSLVNNVRIGLRVPLNGNAPSLPTLESV
                     YPNANWHEREIWDMFGIRFEGHSDLRRILMPYEWEGHPLRKDYPLGYEEVQFTFNYDE
                     IDARKPYAKE"
     gene            complement(597..1079)
                     /locus_tag="AC16V7_16065"
     CDS             complement(597..1079)
                     /locus_tag="AC16V7_16065"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HIR6258689.1"
                     /translation="MGIEQKLGNMGVVTIKLEQAVNWARTNAMWPMLFGLACCAIEMM
                     SAQASNYDMSRFGMELMRASPRQSDLMIVAGRVSRKMAPVLRRLYDQMPDPKWVVSMG
                     DCASCGGVFNNYAIVQGVDEVVPVDVYVAGCPPRPEALIHGIMTLHEKVRGEKIKDWA
                     "
     gene            complement(1070..1426)
                     /locus_tag="AC16V7_16070"
     CDS             complement(1070..1426)
                     /locus_tag="AC16V7_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HIR6258690.1"
                     /translation="MLTQYLPIAILVVLATGLAFLVIALGHLFGPRRPTERKSLPYES
                     GMIPIGPGTRRMPVRFYLVAVLFILFDIEVIFFLPWAIVLRQLKLFGLLEMFVFVFIL
                     LVGYFYAWKKGALEWE"
     gene            complement(1553..>1960)
                     /locus_tag="AC16V7_16075"
     CDS             complement(1553..>1960)
                     /locus_tag="AC16V7_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR6258691.1"
                     /translation="PLPIDLRRRLARAVAKVNRILQAAEEPMSLESPVGSEDSSQLGD
                     FIEDEEALEPMDAAAREMLRESVKNALAVLSERERQVLELRFGLMDGKDHTLEEVGQY
                     FNVTRERIRQIESKALRKLRHPTRSRHLRDYLG"
BASE COUNT          486 a          535 c          499 g          440 t
ORIGIN      
        1 acacggtttc gccttcgata gcccgagctg agactaggtg gcgtagctca tcgagtgaga
       61 cttcagtttt ttccaacatg cctattcctt cgcgtagggc tttcgagcgt caatttcatc
      121 gtaattaaat gtgaactgca cttcttcata gcctaaggga tagtctttgc gcagggggtg
      181 cccctcccat tcatagggca tgagaatgcg gcgtagatcg gagtgacctt cgaagcgaat
      241 gccaaacata tcccagatct cgcgctcatg ccagttagcg tttgggtata cgctttcgag
      301 ggtaggcaag ctgggggcgt tgccattcaa tggcacgcgc aggccgatac gcacattgtt
      361 caccagcgag tagagtaggt aaaccaaatg gaagcgcggc tccagctcag gccagtaatc
      421 cactgcggtc tcgccagaaa gcatctcgaa attgaactcg tcgcgcaacg cacggcagac
      481 ttcgacgatc tggtcaggag agacaagcag gctgacatcg ccgcggtact cgctgatatt
      541 agcgccaaag cgctcttgta tcgcttgagc agcagattgt aagtgttcgt ccatgattaa
      601 gcccaatcct tgattttctc gccgcgcacc ttttcgtgca gggtcataat accgtgaatc
      661 agggcctcag ggcgtggcgg gcagcccgcc acgtaaacat ccactggcac cacttcatcc
      721 acaccttgta caatggcgta attattgaaa acgccaccac atgaggcgca atcacccatt
      781 gagaccaccc acttgggatc aggcatctgg tcatacaggc ggcgtaaaac aggcgccatc
      841 ttgcggctga cgcgccccgc aacgatcatc aaatccgatt ggcgcgggct agcgcgcatc
      901 agttccatgc cgaagcggct catgtcgtaa ttggaagcct gcgctgacat catttcgatg
      961 gcgcagcaag ctaacccaaa aagcatcggc cacatggcgt tggtgcgagc ccagttgaca
     1021 gcttgctcaa gtttgatggt caccacgccc atgttgccca gtttttgctc tattcccatt
     1081 ccagcgctcc tttcttccac gcataaaagt aacctaccag taatataaag acgaagacaa
     1141 acatctcaag caatccgaat agcttcaact ggcgcaggac gatagcccaa ggcagaaaaa
     1201 agatcacctc aatatcgaac aggataaaca acaccgccac cagataaaac cgaactggca
     1261 ttcggcgagt gccaggtccg atgggtatca ttccagattc atagggaagg gacttgcgtt
     1321 ctgtggggcg tcttgggccg aagaggtggc ctagtgctat gaccaagaag gctaaaccag
     1381 tagccagaac gactaaaatg gcgatgggga ggtattgcgt cagcatagtt tgcctcgaaa
     1441 gtgaattcgt cgattgttaa tctaatctgg tctaagttgt aagtttatca caggtggtaa
     1501 atttcacaaa ttaaaaatag ataaatgacc agtttgccaa aaacgcaaca tgtcagccca
     1561 aataatcgcg taaatggcgg ctacgcgtag gatggcgcaa cttccgcaat gccttggatt
     1621 caatctggcg aatgcgctcg cgggtgacat tgaaatattg ccctacttct tccagtgtat
     1681 ggtccttgcc atccatcagg ccaaaacgca actcgagcac ctgtcgctcg cgctctgaga
     1741 gcaccgccag tgcattcttt actgattcgc gtagcatctc acgcgccgcg gcatccattg
     1801 gctctagggc ctcctcatct tcaatgaagt caccaagctg gctgctgtct tcgctgccga
     1861 ccgggctttc cagcgacatc ggttcttcag cggcctgcag gatgcggttc accttagcca
     1921 ccgcacgcgc cagcctgcgg cgaaggtcga ttggcagggg
//
LOCUS       DBNHOH010000893         4838 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1083572, whole genome shotgun sequence.
ACCESSION   DBNHOH010000893 DBNHOH010000000
VERSION     DBNHOH010000893.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4838)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1083572"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1544)
                     /locus_tag="AC16V7_16080"
     CDS             complement(<1..1544)
                     /locus_tag="AC16V7_16080"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR6258692.1"
                     /translation="MKGKSNKNISTGLAASAQSPASPGVSPLASVHGFITRLRPTGRG
                     SLLLVVLLLVAIPSLAPLFPRNVTPASAPSDRFSAERAMLHLPIIAREPHPAGSPAQA
                     GVRDYLVQQLSAIGLETEVQQAGRIENVVARLHGSEPTGAIVVLAHYDSVAAGPGAAD
                     NGSGVAALLEIMRALASGPAPRNDVIALFDDSEERGPYAGTRAFVLGHPWMADVRVAI
                     SLDTAVRGPISTNETGPNNGWLVQALARAYTGGAWTSASGGGGYDYTPFREAGIQGLA
                     LEDNYPFKEQHTAQDIPEIVSAASVQQMGEQTLSITRELGKLDLSNPYAEQETYFSVS
                     GLGLVHYPEAWAVPLAISASVLLVIAFGLALWRGFATWRGLVVALLAILVTSGLAGLG
                     INALWAGVPDLMGWETSSWAEWPEVIPPYGWLVVAAFALLILGLAIVGYILARHWSAR
                     SDYSLVGLALFLIPAVALATGIPRASYVPVWSVLIASLCWIAAVAAGRKRMAWSVDMG
                     TVLAALP"
     gene            complement(1684..2838)
                     /locus_tag="AC16V7_16085"
     CDS             complement(1684..2838)
                     /locus_tag="AC16V7_16085"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR6258693.1"
                     /translation="MNKKSNQRRYDLDWLRVFAILFVFIYHSSRFFNLEDWHVKNIST
                     YFGVEVWNTFASSWLMPLIFVISGASLFYAMGKVSIGTIGKFIKDKVLRLFVPLMVGA
                     FTHASLQVYLERLTHGQFTGSYFEFIPHYFEGIYGFGGNFNIVGMHLWYLGVLFVFSI
                     VFLPLFLGLKSKIGARLLGRFTDFLAVPGLIYLFAMLILLSWKLIDPDSLLGFDKFGW
                     NLGVYISFFLSGFVFFSSEKLQHSIQKLRWLSLVGLITFTVLLILGQSHRDLIAWFFI
                     LAFLGFGTKQLNISTPLLKYANEAVLPFYILHQTVLLCVGYYVVQWHIPDLLKWLLIV
                     PIAFAIIMGLYEYLVRRNNVMRFLFGMKPLVKPLVSPAIEAQITRATRTT"
     gene            complement(2835..3239)
                     /locus_tag="AC16V7_16090"
     CDS             complement(2835..3239)
                     /locus_tag="AC16V7_16090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR6258694.1"
                     /translation="MTTSTTIKRTKTATQVLIVDDAPLVRRELRTLLSLTGELDIVGE
                     ATNGLEAIQQIEELQPDVVLMDLEMPVMDGYEATRQIKAQHPACRVVALTVHSYDEAH
                     KKALQAGVDAFIVKGTPVDKIVQAITERRIKS"
     gene            complement(3506..4294)
                     /locus_tag="AC16V7_16095"
     CDS             complement(3506..4294)
                     /locus_tag="AC16V7_16095"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019350.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6258695.1"
                     /translation="MTYEPRASRYKGSYLIGFILIVVVAIRTILFFQGQTAITVIIFL
                     LSAYSLLYALEPLLSNRLRWYRFIYFPLQTVLAVAITTLRPFTDISNLVYIPIAIQAL
                     HAFSRPVAIPLMTLYAAMLNITLILGMGWAEGLALGLLYLAVCTFLISYDLLYSRSQA
                     DQAESLALLADLQAAHQKLREYADQAEELSVARERNRLARELHDSVSQVIFSITLTSQ
                     AARLLLERNPARVPEQLNSLQEMTSNALQQLRSLITQLRPQQKS"
     gene            complement(4291..>4838)
                     /locus_tag="AC16V7_16100"
     CDS             complement(4291..>4838)
                     /locus_tag="AC16V7_16100"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR6258696.1"
                     /translation="TVQSAHLLLDKEPGRVSGQLERLEELATSAMREIQALVSQLRPR
                     SIADEGLPAALRRLAADRQTRDGLELILDVRDETGLPEPEAVGLYRIVQEALNNVVKH
                     AETRQATIRLDLSEKPAYLEIEDHGQGFDTQAVMSKRGHLGLTGMSERARELGWHLSI
                     ESHPGQGTRIRVEQSLPDGET"
BASE COUNT         1118 a         1296 c         1372 g         1052 t
ORIGIN      
        1 ggcagagctg ccagtacggt ccccatatct accgaccatg ccattcgctt cctgccagcg
       61 gccacagccg cgatccagca aagcgaggcg atgagcacag accacacagg cacgtaggat
      121 gcgcgcggga tgccggtggc taatgcaacc gcagggatga ggaacaatgc cagcccaacc
      181 agagagtagt ctgaccgtgc gctccagtga cgagccagaa tatagcccac gattgccaat
      241 cccaggatga ggagcgcaaa tgcggcgacg acgagccatc catagggagg gatgacctct
      301 ggccattcgg cccagctact cgtctcccaa cccatgaggt cgggcacgcc tgcccaaagt
      361 gcattaatgc cgagtccagc taagccactc gtaaccaata tcgccaagag tgctacgacc
      421 aatccgcgcc aggtggcgaa cccgcgccat agtgctagcc caaacgcgat taccaggagc
      481 acgctagcgg aaatggctag cgggactgcc cacgcctccg ggtaatggac aagccccaat
      541 ccggagacag agaagtacgt ctcttgctcg gcatagggat tactgaggtc gagcttgccg
      601 agctcgcgcg tgatggacag agtctgctca cccatttgtt gcacgctggc ggcgctgacg
      661 atttcaggaa tatcttgcgc agtatgttgc tctttgaatg ggtagttgtc ctccaatgcc
      721 agcccctgga tgcccgcctc gcgaaacggg gtgtagtcat aacctccgcc accactggca
      781 gatgtccagg caccaccggt gtaggcgcgg gcgagcgctt gtaccagcca tccattattt
      841 ggaccggtct cgttcgtcga gattggccca cgaacggcgg tgtctaagct gatggccacg
      901 cgcacatcag ccatccaggg atgccctagc acgaaggccc gcgtcccagc atatgggccc
      961 ctctcctcgc tgtcatcgaa cagcgcgatc acgtcgttgc gcggcgctgg gccggaggcg
     1021 agggcgcgca tgatctctag cagcgcagct accccgctgc cattgtccgc cgcgcctggt
     1081 ccagctgcca ccgagtcata gtgggcgagc accacgatag cgccggttgg ttcgctgccg
     1141 tgcaagcggg cgaccacgtt ttcgatacgt cctgcctgct ggacctcggt ctccaggcca
     1201 atggcagaaa gctgctggac gaggtagtct cgcacgcccg cctgcgcagg tgagccggcg
     1261 ggatgcggtt cccgagcgat gatcggcagg tgcagcatag cacgttcggc tgagaaacga
     1321 tccgatggcg cgctggcagg tgtaacgttt cgagggaaga gtggcgcgag cgacgggata
     1381 gccaccagca aaagcacgac aagcagtagg gatccacggc cggtcggcct gaggcgcgtg
     1441 atgaagccat gcacggacgc aagcggagaa acgccagggc ttgccgggct ttgcgcgctg
     1501 gctgccagac ctgtgctgat atttttattc gatttgcctt tcatggtttt ttctccttct
     1561 caatcgcgat catctgaaag agattggata cccttggttt ggaaggctgc ctgataattt
     1621 ccatggtttg gctgctatac cttgagtggt atgcagtaat tgcgcaaggt tttaacatct
     1681 ggtttaggtt gtgcgagtag cccttgtgat ctgggcttcg atcgcagggg agacgagggg
     1741 tttgaccagc ggcttcatcc cgaagaggaa gcgcatgacg ttattccgcc gcaccaggta
     1801 ctcgtacagc cccatgatga tggcaaacgc gatggggacg atcaacagcc atttcagcag
     1861 atccgggatg tgccactgca cgacgtaata tcccacacag agcaatacgg tctggtgcag
     1921 gatgtagaag ggcaataccg cctcgttggc gtacttaagc aatggcgtgc tgatgttcag
     1981 ttgcttcgtc ccgaagccta gaaatgccag gataaagaac caggcgatca agtccctatg
     2041 ggattggcct aagattaata aaaccgtgaa ggtgatgagc ccgacgagtg agagccagcg
     2101 caacttttgg atactatgct ggagcttttc actcgagaaa aatacaaatc cggataggaa
     2161 gaaggagata tataccccca ggttccagcc aaatttgtca aatcccagca aactatctgg
     2221 gtcaataagt ttccaggaaa ggagaatcag cattgcaaag aggtagatca accctggcac
     2281 agccaggaaa tcagtaaacc tgcccagcag gcgggcgcca atcttgcttt tcaaccccag
     2341 gaaaagcggc aggaagacga tgctgaatac gaacaacacc ccaagatacc aaaggtgcat
     2401 gcccactatg ttgaaattgc caccaaaacc atatatccct tcaaaatagt gcgggataaa
     2461 ctcgaaatat gagcccgtaa attgcccatg tgtcaggcgc tccaggtaga cctgcagtga
     2521 ggcgtgggta aaggcgccca ccatcagggg cacgaataag cgcagtacct tatctttgat
     2581 gaattttcca atcgtgccga tactgacttt ccccatggca taaaacaggc tggcgcctga
     2641 gatgacgaag atcaatggca tcaaccagct tgatgcaaat gtgttccaca cctccacccc
     2701 gaagtacgtg ctgatgtttt ttacgtgcca gtcttccaag ttaaaaaagc ggctggagtg
     2761 gtagataaag acaaagagaa tggcaaatac ccgcagccag tctaggtcgt accttctttg
     2821 attagatttc ttattcatga cttaatcctt ctttctgtaa ttgcctgtac tattttatct
     2881 accggtgtgc ctttcacgat gaaagcatcc acaccggcct ggagagcttt cttatgcgcc
     2941 tcatcgtagc tgtgtacggt cagggcgact acccggcagg cggggtgttg ggctttgatc
     3001 tgtcgagtag cttcgtagcc gtccatgact ggcatctcca aatccatcaa aaccacatcc
     3061 ggctgtagct cttcgatctg ctgaatggct tctagaccgt tggttgcctc gccaacgatg
     3121 tctagttctc cagtaagaga caacaaagtg cgcagttccc ggcgcaccag aggggcatcg
     3181 tctacgatca gtacctgggt ggcagtcttg gttcgtttga tagttgttga tgtagtcatt
     3241 actgtttctc ttttcttggt gaaagcttgc tctcaggaga ttacgatctt aatataggcg
     3301 cgtttgcagc aaaatacatc tggctagagt tgtgttttgt catcggtcat ggggtcgatt
     3361 tttatggtca gccttgggat ggcattgctc catcccacgt cttaggactt tgggagtagc
     3421 tcaggtttgg cgctatccgc gaagaaagac gggcaattat gtgctttccc gctcaagctg
     3481 gaaaacccat attatctttt cttagtcagg atttctgttg ggggcgtagc tgggtgatga
     3541 gcgagcgcag ttgctggagg gcgttgctgg tcatctcctg caaactgttg agctgttctg
     3601 gaacacgagc cggatttcgc tccaggagca ggcgagctgc ctgcgaggtg agggtgatgc
     3661 tgaaaatcac ctggctgacc gaatcgtgca gttcgcgcgc caggcggttg cgttcgcgag
     3721 ccacagacag ttcttcagcc tgatcagcgt attcgcgtag cttttgatgg gctgcctgca
     3781 gatccgcgag caatgccagg ctttcggcct gatcagcttg cgagcggcta tagagcaaat
     3841 cgtacgaaat cagaaaggtg caaacggcca gataaagcaa accgagcgcc aaaccctcag
     3901 cccaccccat acccaggatc aacgtgatat tgagcatggc cgcgtagagg gtcatcaagg
     3961 ggatagctac cgggcgcgag aaggcatgca gggcttgtat ggcgatcgga atatacacga
     4021 gattcgatat atctgtaaag gggcgcaaag ttgttatcgc tactgccagg acggtttgaa
     4081 ggggaaagta tataaagcga taccagcgca gacgattgga tagcagcggt tcaagtgcat
     4141 agagcaggct gtaggctgac aggaggaaaa tgataactgt aatagcagtc tgtccctgga
     4201 aaaaaagaat cgtccttatg gcgaccacaa cgatcaaaat gaacccgatc aggtagctgc
     4261 ctttgtatct gctggctcgg ggttcgtatg tcatgtctca ccgtccggca agctctgctc
     4321 gactcggatg cgcgttccct gacctgggtg agactcgatc gacaaatgcc aacccagctc
     4381 gcgcgcccgc tcagacatgc cggtcaggcc gaggtggccg cgcttgctca tgactgcctg
     4441 ggtatcgaac ccttgcccgt gatcctcgat ttcgagatag gcgggcttct cggacaggtc
     4501 gaggcggatg gtcgcttggc gcgtctcggc gtgcttgacg acgttattga gcgcctcctg
     4561 aacgatgcga tataaaccga ctgcttctgg ttcgggcagg cctgtctcgt cgcgcacgtc
     4621 caggattaac tccagcccat cgcgggtctg acggtctgca gccaacctgc gcagggcagc
     4681 tggcaatccc tcgtcggcaa tcgacctggg gcgcagctgt gagaccaaag cttggatctc
     4741 tcgcatggca ctcgtagcca actcttctaa ccgttccagc tgaccagaaa cacgtccagg
     4801 ttctttatcc agcagcaggt gggcagattg gacggtca
//
LOCUS       DBNHOH010000894         8270 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_249465, whole genome shotgun sequence.
ACCESSION   DBNHOH010000894 DBNHOH010000000
VERSION     DBNHOH010000894.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8270)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8270)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8270
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_249465"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="AC16V7_16105"
     CDS             complement(<1..309)
                     /locus_tag="AC16V7_16105"
                     /inference="COORDINATES: protein motif:HMM:NF018649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HIR6258697.1"
                     /translation="MVTLWLLSLWLRDASIVDIFWGLGFVCVAWLYFALTPQGFSARK
                     GVLSLLVTVWGLRLSVHIFMRNRGKGEDFRYRGWREQAGGKWWWQSFFKVFLLQGVLL
                     W"
     gene            complement(358..618)
                     /locus_tag="AC16V7_16110"
     CDS             complement(358..618)
                     /locus_tag="AC16V7_16110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258698.1"
                     /translation="MMRIVLDRRSCQCYDQACESHFGWHFLRDDITPVDCTLEMVDDQ
                     RAETTFLILDKDGIDKELVVNDENWGIAYDSWRQAWEQQQAK"
     gene            complement(673..1554)
                     /locus_tag="AC16V7_16115"
     CDS             complement(673..1554)
                     /locus_tag="AC16V7_16115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258699.1"
                     /translation="MSDERGPWYLITGLVLGAVMGVLYAWVLQPVQYTDTTPASLRAD
                     FKDQYRAMIALAYMANGDLVRAGARLDLLEDEDVYKTLAEQAQRTLAGGSASQEARAL
                     GMLAVALGQSPSTLSAPGGGEPVSVPQETQVITPTLSFSAVLTRTLPAATEESTAQPT
                     ETSPTDSPTITPLPTRQPTPTIGSPFVLKSEELSCNPAQETPLIIVQAYDAAGQGIWG
                     VEAVVNWEDGESHFFTGLKPELGIGYADFTMTPGVVYTLRLAEGGQTIPNLTPKECEA
                     EGGERYWGSWLLVFAQP"
     gene            complement(1551..1907)
                     /locus_tag="AC16V7_16120"
     CDS             complement(1551..1907)
                     /locus_tag="AC16V7_16120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258700.1"
                     /translation="MSRWIRFFLAIALGAAGGLFYGWVVSPIQYIDTTPDTLRIDYKS
                     DYILMVAETYSVDKEMALAARHLGLLGDTAPVEMVREAVLFAESQGYTDADVALMRQL
                     LTDLQSWIPSLETPSP"
     gene            complement(1921..3045)
                     /locus_tag="AC16V7_16125"
     CDS             complement(1921..3045)
                     /locus_tag="AC16V7_16125"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HIR6258701.1"
                     /translation="MDIQTVSDLDTPPRILLGPGPSMVSPRVLRAMAQPLLGHLDPQF
                     LALMDEVQQLLRYVFQTQNELTIPVSGTGSAGMEAALCNFIEPGDRVLVAINGYFGER
                     MVDMAGRYGAQVDRLECPWGQVFDPGDIEAALRGKQYKLVALVHAETSTGAHQTGIAE
                     IAAAAHRHGALLVLDTVTSLGGVPVEIDAWEVDVAYSGTQKNLSCPPGLAPLTVSPRA
                     RDVLRGRKTPVANWYLDLSMLDKYWGHDRTYHHTAPISMNYALREALRIVAEEGLEPR
                     FARHRLHAEMLWDGLEALDLPPFVAIEYRLPTLTTATLMPSIDDAAVRRRLLDEYNIE
                     ISGGFGPLKGKVWRIGLMGFSSRRENVTLLLAALQHILSG"
     gene            3360..3620
                     /locus_tag="AC16V7_16130"
     CDS             3360..3620
                     /locus_tag="AC16V7_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HIR6258702.1"
                     /translation="MDIIKVSGRSRTSAVAGAIAGVFRENKRVEVQAIGASAINQAVK
                     AIALAKSYLAEDGYSIVCIPEFVDVEIEEKIRTAIKFVIEQR"
     gene            3877..4824
                     /locus_tag="AC16V7_16135"
     CDS             3877..4824
                     /locus_tag="AC16V7_16135"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012958.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6258703.1"
                     /translation="MLTLAILLCLGLASCAAKDPSLGTPLSTSDIAPSPLPTQTITPT
                     ITPSPLPSNTPSPLPPTLTPIPSLTPSPTPLACWGEPGNKEHASLSSDLLRLPLEYTV
                     YLPPCYDQQKDRRYPVLYLIHGQNYNQDQWDRLGADETADALIAAGEVPPFIIVLPRD
                     RSWAQPTEDMFGQVVAEELVPTIDEGYRTLSERAYRAIGGLSRGAGWAVHLGLSRWEL
                     FGAIGGHSLPVFWSDTPYIRRWLKDIPDDALPRIYLDIGEKDRPNILDSAIWFEKLLT
                     TEGIPHEWHLYAGYHEEAYWESHVDEYLRWYADGWMGSG"
     gene            4892..5179
                     /gene="gatC"
                     /locus_tag="AC16V7_16140"
     CDS             4892..5179
                     /gene="gatC"
                     /locus_tag="AC16V7_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005551236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIR6258704.1"
                     /translation="MPLTLKEVEHIANLARLDLTDDEKARYREQLSAILDYFTQLSEL
                     DTGGIPPTSSVLSPRSVLRDDEPHPGLSLEDLLRNAPDAEAGQFRVPFILE"
     gene            5256..6731
                     /gene="gatA"
                     /locus_tag="AC16V7_16145"
     CDS             5256..6731
                     /gene="gatA"
                     /locus_tag="AC16V7_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIR6258705.1"
                     /translation="MPELTDLTLTEAIAALRAGDTTSHELTTAYLERIQRLDSSLHTY
                     LTLTPELALQQAGEADRHISAWRKAPGPLAPLMGVPLAVKDVLCVAGVRTTAGSRILE
                     NFVPPFNATAVERLRAAGVVILGKTNTDEFAMGSSTENSAYGVTRNPWNPEHVPGGSS
                     GGSAAAVAARLAPAALGTDTGGSVRQPASFCGVTGLKPTYGRVSRYGLIAYGSSLDVV
                     GVFGHSAADLAPIFTQMAGYDARDATTVEQPVPQISLNNQVDLEGLRVGVPDEYFISG
                     IQPEVEAAVRQAVSVLESLGAQARPVSLPHTRYALPVYYLIAPAEASANLARYDGIRY
                     GPRMPADTMWDVFRHTRGEKFGAEVKRRIMLGTYALSAGYYDAYYGQAQKVRTLIKGD
                     FERTLQDVDVIAAPVAPTTAFKIGGHGDDPLSMYLEDVFTLPANLAGVPGLTFPVGFD
                     GMGLPIGMQLMGAHFGEDVLFKVAHAYQQVTSWHQAIPALS"
     gene            6769..7878
                     /locus_tag="AC16V7_16150"
     CDS             6769..7878
                     /locus_tag="AC16V7_16150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258706.1"
                     /translation="MKSGQRNAIIVLSAIAALSLTFAIVIFLLLYPGWAKRSPLPITP
                     TSVQVEMLENTLPADTSPTLDTGSSPLDGTYTKVDPSWPQWWLCRRCADYRLAGGIWK
                     LVFDKGMMHIYYDMTGWRSMASFNISGDRLHIFNDPYCPDDEGEYTWEQGGGQIKLEV
                     IDDTCSFQLRGKNLSNQPWLTCLPPEKTTHPASDWQAPPGCEGSPTMPEPAPASNLPI
                     NVTVHGGDSRFFKKPPDVFANANNADIPSPEGIYVTYHDQSIPYGLSRVLWWEGDWIQ
                     ASTELPFASMGVQILGDPPIGWARLLFDGVEVWRGNTSAIWSYHGRHGGYVEISGFNP
                     GTHVIRAESMAFDYRPVTVASFGFSYHEGVKSQEP"
     gene            7903..>8270
                     /locus_tag="AC16V7_16155"
     CDS             7903..>8270
                     /locus_tag="AC16V7_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HIR6258707.1"
                     /translation="MMRSPLPSAPNEFMSWSWGQIEPYYQELADRQLNSHTLQAWLTD
                     WSNLSKMLYEMFWRLNVAVTVNTADHEAERRYIAFLDEIQPPSQAAEQKLKQKLLASS
                     LSPDDFEIPLRTMQTEAQLFR"
BASE COUNT         1822 a         2481 c         2232 g         1735 t
ORIGIN      
        1 ccacagcagc acaccctgta gcaggaacac cttgaagaag ctctgccacc accacttgcc
       61 gccagcctgc tcccgccagc cgcggtagcg gaagtcctcg cctttgccgc ggttgcgcat
      121 gaagatgtgc acagacaagc gcagccccca gaccgtgacc agcaggctca gcaccccctt
      181 gcgagcagaa aacccctgtg gcgttagagc gaaatacagc caggcgacgc acacaaagcc
      241 cagcccccag aatatatcca cgatgctggc gtctcgcagc cacaggctga gcagccacag
      301 ggtcaccatc agcccgagga tcaggcacgc gccgtacagg taagccatta tttcatctta
      361 cttcgcctgt tgctgctccc aggcctgtcg ccaggaatcg taggcaatcc cccaattctc
      421 gtcattcacg accagttcct tgtctatacc atccttatcc aggatcagaa aagtggtctc
      481 cgcccgctgg tcatccacca tctccagggt gcagtccacc ggtgtgatgt catcccgcag
      541 gaaatgccag ccaaaatgag actcgcatgc ctggtcataa cactggcaag agcgtcggtc
      601 caaaacaatt ctcatcataa cctcctaaat tttcgtaaga tgcttttatc tatccctcag
      661 tattcatatc aattacggct gtgcgaaaac cagcagccag gaaccccaat agcgctcccc
      721 accttcagcc tcgcattcct tgggggtcaa gttggggatg gtttgcccgc cttctgccag
      781 gcgcagggtg tagactacac ccggggtcat ggtgaaatct gcatacccta tacccagttc
      841 gggcttcagg ccggtgaaga aatgactttc gccatcttcc caatttacca cggcttccac
      901 tccccagatg ccctgaccag cggcgtcata cgcctgcacg atgatcagcg gtgtttcttg
      961 ggcagggttg caggacagct cctcgctttt caggacaaag ggcgacccaa ttgtcggtgt
     1021 cggctggcgc gtaggcaggg gcgtgatggt gggcgaatcc gtggggctgg tttcggtcgg
     1081 ctgggctgtg ctctcctcag ttgccgccgg aagtgtcctg gtcagaacag ccgaaaaaga
     1141 cagggtgggg gtgatcacct gtgtctcctg agggaccgag acaggttcac cccctccagg
     1201 cgcggacaaa gtggacggcg actgccccaa ggcaactgcc agcataccca gagcgcgcgc
     1261 ctcctgcgag gcgctgccac ccgccagggt gcgctgggct tgctcagcca gcgtcttgta
     1321 cacatcctca tcttcgagca ggtccaagcg cgccccagcc cgcaccaggt cgccattagc
     1381 catgtatgcc aaggcgatca tagccctgta ctggtctttg aagtctgcgc gcaacgaggc
     1441 gggggtagtg tcagtatact ggactggctg caaaacccat gcataaagca ctcccatcac
     1501 tgcaccgagc accaagccgg tgatcaggta ccagggaccg cgctcatcgc tcatggcgaa
     1561 ggtgtctcca gggacggaat ccaggactga agatcggtca ggagctggcg catcaaggcc
     1621 acatccgcgt cggtgtagcc ctggctttct gcaaagagca ccgcttcacg caccatctcg
     1681 accggcgctg tatctcccag caatcccagg tggcgggcag ccaacgccat ctccttatcg
     1741 acgctgtatg tctctgccac catcaggatg taatcgctct tgtaatcgat gcgcagtgtg
     1801 tcaggggtgg tatctatgta ctgaattggg ctgactaccc agccatagaa tagccccccc
     1861 gctgcgccaa gagcaattgc cagaaaaaat cggatccagc gagacatcgt cgatgctgcc
     1921 ttagccagac agtatgtgct gcaaggccgc caacagcagt gtcacgttct cgcgccggct
     1981 gctgaagccc atcagtccga tgcgccacac cttgcctttc aggggtccaa agccccctga
     2041 gatttcgatg ttgtactcat ccaggagccg gcggcgaacg gcggcatcat caatagatgg
     2101 catcagagta gcggtagtca acgtaggcag gcggtactct atagccacaa agggcggcaa
     2161 gtccagggct tccagcccat cccaaagcat ttctgcgtgc aggcgatggc gagcaaagcg
     2221 cggttccagc ccctcttcag ccactatgcg cagcgcctcg cgcagggcat agttcatgct
     2281 gattggggcg gtgtggtgat aggtgcggtc gtgcccccag tatttgtcca gcattgaaag
     2341 atccagatac cagtttgcca ccggggtctt gcgcccgcgt agcacatcgc gagcgcgtgg
     2401 cgagacagtc aatggagcca ggccgggtgg gcaagacagg tttttctgcg tgccgctgta
     2461 agccacatcc acttcccagg catcgatctc gactggcacg ccccccagag aggtgaccgt
     2521 atccagcacc agcagcgccc cgtggcggtg agcggctgca gcgatctcag cgatgcccgt
     2581 ttggtgggcc cctgtagagg tttcagcgtg caccagggcg accagtttat actgttttcc
     2641 tcgcagggct gcctcaatat ccccagggtc aaagacctgc ccccaagggc actccaggcg
     2701 gtccacctgg gctccataac gcccagccat atcgaccatg cgttcgccaa agtacccgtt
     2761 gatggcgacc aagacgcgat ccccaggttc gataaaattg cataaggcag cttccatgcc
     2821 tgcgctgcca gtgccagaga ccggaatggt gagctcgttt tgcgtctgga aaacatagcg
     2881 cagcagttgc tgcacctcat ccatcaaggc cagaaattgt gggtccaggt gacccagcaa
     2941 gggctgagcc atggcgcgca ggacacgcgg cgagaccatg ctgggacccg gcccgagtaa
     3001 tattcgggga ggggtatcca gatcggagac agtttgaata tccatgaaat tccttttgat
     3061 agattagatg atatgaaaga aggtcaccgc tgctctcaga gcaaaagtaa ctatgctcaa
     3121 ttgtaacctt acggttgtcg aaccgcgtaa taacaatagt ttacaacaaa aatcgaactt
     3181 caccaagaat tgaaatttca tctgccaggg ttttaattgc cagggctatc aattctgaga
     3241 aaattcgcga atcatcttat tgaaaactgc ataaatatct tgtacaatga taatgaaagc
     3301 gttaggtcgt ctcacagcgg ccacaacaca gcgaacaatg gtttcgggga ggaagcggca
     3361 tggacatcat caaagtgtca ggcaggtcac gcacttccgc tgtggcgggg gctatcgcag
     3421 gagtttttag ggagaataag cgtgtcgaag tgcaagccat tggagccagc gctattaatc
     3481 aggctgtcaa agcgatagca ttggcgaaga gttatctagc agaagacggc tatagcattg
     3541 tatgcatccc agaatttgtg gacgtggaga tcgaggagaa aattcggacc gccattaagt
     3601 ttgtcatcga acagcgctaa aataccaccg atcaatcgac ccaggcggtc gccaaagcaa
     3661 agcattctgt gaaatcagaa ctgccgttgt tgtgccacgc atttattcac tgcgtggccc
     3721 tttcgtttct ggacgggctg tttatctttt gatacacttg gcgggatggc gatatcccca
     3781 atatcaaaaa aactaattac ccaatctcat catccaaccc tacttccccg ccactccgct
     3841 ggaagcggtg gggggaatta tcacatcagg gtctctttgt taacgcttgc gatcctgctc
     3901 tgcctgggac tggcaagttg tgcagccaaa gacccatccc ttggcacacc cttatcgaca
     3961 tcagacatcg ccccctctcc cctacccacg caaaccatca cacccaccat cacgccctct
     4021 ccactgcctt ccaacacccc ctcgccgctg cccccaaccc tcacacccat cccatccctg
     4081 acgccttctc ccaccccgct ggcatgctgg ggggagcccg gcaacaaaga gcacgccagc
     4141 ctatccagcg acctgctgcg gctgccgcta gagtacacgg tgtacctgcc tccatgttac
     4201 gatcaacaaa aagatcgccg ctatcccgtg ctgtacctga tccacgggca gaactataac
     4261 caagaccagt gggaccgtct gggcgcagat gagaccgccg acgccctgat cgctgcgggc
     4321 gaagtgcccc ccttcatcat tgtattaccc cgagaccgct cctgggcgca acccaccgaa
     4381 gatatgtttg ggcaggttgt ggcagaagag ctagtcccca ccattgacga gggttaccgc
     4441 acgctctcag agcgagctta ccgcgccatt ggaggcttat cgcgcggtgc gggttgggcg
     4501 gtgcacctgg ggctctcgcg ctgggaactc ttcggggcga tcggcgggca tagcctgccg
     4561 gtattctgga gcgacacgcc ctatatccgt cgctggctga aggatatccc ggatgatgcc
     4621 ttgccgcgca tctatctgga tatcggcgaa aaggatcgac cgaacattct cgactcagcc
     4681 atctggtttg agaaactgct caccacagag ggcatccccc acgagtggca cctgtacgct
     4741 ggatatcacg aggaagccta ctgggaatcg cacgtagatg agtatttgcg ctggtacgca
     4801 gatggctgga tgggttcagg gtaagcacag caagtcacca ggtgccaatc acagccctgc
     4861 aatcgatgac ctgaatgata taatccctgt tatgcccctc actcttaagg aagtcgaaca
     4921 tatcgccaac ctggctcgcc tggatctgac ggacgatgaa aaggctcgct accgcgagca
     4981 gctctcagcc atcctggatt atttcaccca actgagtgag ctggacacag gcggcatacc
     5041 acctacctcc agcgtgttat caccccgcag cgtcctgcgc gatgacgagc cgcaccccgg
     5101 tctgagcctg gaagatttac tgcgcaacgc gcctgatgcc gaggcaggcc agttccgcgt
     5161 cccgtttatt ttggagtgaa ttagccgaga tttgagcggg aaaacaggaa atacctgttt
     5221 attccattcc acacgcaccc gaatttgagt atcccatgcc cgagttgacg gatctgacgc
     5281 tcactgaagc catagccgcc ctgcgcgccg gggatactac cagtcacgag ctgacgacag
     5341 cctacctgga gcgtatccaa cgcttggact ccagcctgca cacttatctc accctgaccc
     5401 ccgagctggc actgcagcag gcaggtgaag ccgatcgaca cataagcgcc tggcgcaaag
     5461 cgcccggtcc cctggcgcca ctcatgggcg tgccgctggc ggtaaaggat gtgttgtgcg
     5521 ttgccggcgt gcggactacc gccggttcgc gcatcctaga gaactttgtg cctcccttca
     5581 acgccaccgc ggtggagcgc ctgcgggctg cgggcgtggt gatattgggc aaaaccaata
     5641 ccgatgagtt cgccatgggc tcttccactg agaactcggc ctatggcgta acccgtaacc
     5701 cctggaatcc cgagcatgtc cctggtggct ctagcggggg cagcgcggca gccgtggcgg
     5761 cgcgcctggc tcctgcagcc ctaggcaccg atactggcgg cagcgtgcgc cagccagcct
     5821 ccttctgcgg cgtgaccggc ttaaaaccca cttatgggcg ggtctcgcgc tatgggctga
     5881 tcgcctacgg ttcttccctg gatgtcgttg gggtatttgg gcacagtgcc gccgatctgg
     5941 ctccaatttt cacccagatg gctggttatg acgctcgcga tgccacaacc gtagaacagc
     6001 ccgtcccgca aatatcattg aataatcagg tggatctgga gggcttgcgc gtgggcgtcc
     6061 ctgatgaata cttcatctcc ggtatacagc ccgaggtgga ggcggctgtg cgccaggccg
     6121 taagcgtgct ggaatccctg ggcgcccagg ccaggccagt gagtctgccc catacccgct
     6181 acgcattgcc ggtctactac ctgattgccc ccgcagaggc ttccgctaac ctggcgcgtt
     6241 acgacggcat acgctatggg cctcgcatgc ctgccgacac catgtgggac gtgttccgcc
     6301 acacgcgtgg cgagaaattc ggcgcggagg tcaagcgccg aatcatgtta ggtacatacg
     6361 ccctctccgc tggctattac gacgcctatt acggccaggc acaaaaggtt cgtaccttaa
     6421 tcaagggtga cttcgagcgc acccttcagg atgtcgatgt gattgcagcg cctgtcgccc
     6481 caaccaccgc ttttaagatt ggtgggcacg gtgatgaccc tctctccatg tatcttgaag
     6541 atgtgttcac cctgcccgcc aacctggcgg gcgtgcccgg gctgactttc ccggtgggct
     6601 tcgatggcat gggattgcct atcggcatgc agctgatggg ggcgcacttc ggcgaagatg
     6661 ttttgttcaa agtggcccat gcctaccagc aggttacgag ctggcatcag gcgatcccag
     6721 ctttaagcta aggaccatga tgcatttatc cgttctgagc acgggatcat gaaatctggg
     6781 caacgcaacg cgatcattgt tctatccgca atagctgcgc tcagcctcac cttcgccatt
     6841 gtaatattcc tgttactcta tcctggatgg gcgaaaagat cccctctgcc aatcacccca
     6901 accagcgtcc aggttgaaat gttggagaat acccttcccg cagatacatc ccctaccctt
     6961 gataccggct ctagcccctt agatgggacc tataccaagg tcgatccgtc ttggccccag
     7021 tggtggctgt gccggcgctg cgcggactac cgcctcgcgg ggggtatttg gaaactggta
     7081 tttgataaag gcatgatgca catctattac gatatgaccg gctggcggag tatggcttca
     7141 tttaatatct ctggtgatcg tctgcacatc ttcaatgatc cttactgtcc agacgatgag
     7201 ggagagtaca catgggaaca gggtggcggg caaatcaagc tggaagtaat cgatgacacc
     7261 tgctcttttc aactgagagg caagaatctg agcaaccagc cttggctcac ctgtctgccg
     7321 ccagagaaga caacccaccc tgccagcgac tggcaggcac cgcccggttg tgagggcagc
     7381 ccaaccatgc ccgagcctgc acctgcatca aatctaccaa tcaatgttac tgtccatggt
     7441 ggcgacagcc gtttttttaa gaaaccgccg gatgtctttg ccaatgcaaa caatgccgat
     7501 atcccttcac cagaaggcat ttatgtaact taccatgatc agagtatccc atatggcttg
     7561 agtcgcgtcc tgtggtggga aggtgattgg atccaggcct ccacggaact gcccttcgct
     7621 tctatgggag tgcaaatcct gggtgaccct ccgattggct gggcacgcct ccttttcgac
     7681 ggtgttgaag tatggcgcgg aaacacttcc gcgatttggt cctatcatgg tcgccatggt
     7741 gggtacgtag aaatctccgg atttaacccc ggaacgcacg tcattcgtgc ggagagtatg
     7801 gcttttgact accgtcctgt aaccgtggca agttttgggt tcagttatca tgaaggtgtc
     7861 aagagccaag aaccttagaa catattttat aaggaaggaa agatgatgcg atctccgctt
     7921 ccaagcgccc ccaacgaatt tatgtcctgg tcctgggggc agatcgagcc ctactaccag
     7981 gaattggctg accgccagct taactcgcac actctgcaag cctggttgac ggactggtcg
     8041 aacctctcca agatgcttta tgaaatgttc tggcggctca acgtggcagt gacggtgaac
     8101 actgccgatc acgaagccga gaggcgctat atcgccttcc tggacgagat ccagccccct
     8161 tcgcaggccg ccgaacagaa gttgaagcaa aagctgctgg ccagctccct gagcccggat
     8221 gactttgaga tccccctgcg caccatgcag accgaagcgc agcttttccg
//
LOCUS       DBNHOH010000895         6064 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_506414, whole genome shotgun sequence.
ACCESSION   DBNHOH010000895 DBNHOH010000000
VERSION     DBNHOH010000895.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6064)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6064
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_506414"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1643)
                     /locus_tag="AC16V7_16160"
     CDS             complement(<1..1643)
                     /locus_tag="AC16V7_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258708.1"
                     /translation="MQIRSRQYFTIAVIIVINIAIGCIVVTDYGESRDEHLRIDYAER
                     SLAAYTHGKRVSVDEKGPSYVMLALIGSRALRAVWGSLQPIEAWHFMHFLSFLMGVCF
                     LYIICRHYMSEGAALATVLLFNTQPLLWGHAWINPKDIPFMSFFLGSVALGRPMASLS
                     GSAAQSSGYASRGSSSADQADFSSLADTLSTDWRQASPRKRYLLIGACIFLLALLFGL
                     LLADAPIRDWIANLVARAYADGNSSLLGRLFNRLAENTGSIPVELYIQKGQAAYRRLL
                     AGYAMAVPLLWLALAGLLLPRLSKRVWRERVRPFLGAIVAGAARRQTLLAALFLGLAS
                     AIRAIGPASGLLVAVYCLWKSGRKAVPSLVAYFAIAGLVTYFAWPGLWSSPLQGYLSA
                     FREASDFPWEGKVMFAGVDVPVSELPRSYLPTLLALQFTVPVLVLFMLGLSLAILDFR
                     RNREKRPALALAALWLFAPLAGAIVVMPTMYDNFRQFLFIIPPLFVFAGIGVEKLFLY
                     AQRLGRLSSPDRKPNALSPFLRSASKILILAIALSPGLY"
     gene            complement(1684..3414)
                     /locus_tag="AC16V7_16165"
     CDS             complement(1684..3414)
                     /locus_tag="AC16V7_16165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR6258709.1"
                     /translation="MNPRSAWSTKNWSELSVKGRTAWLQWIMLIVVGLAIAVPRLKAL
                     NRLVTPDEHLWLARSANFYMAISQGKLEWTYLSEHPGLTVMWAGTGGFLVRYPQYRHD
                     GPDRVDSNEFNRYIQNAKGVSALDLLVASRVFMILGHTLVLLVSFCYAIRIISPFPAL
                     ISFLLIAFEPYHVALTRVLHLDGLLGNMLLLSLLAIISFLQGRRTRDLLVSAIAAGLG
                     LLTKSPGILIVPVVGLLTLFALRKDGLGKQERSIPRLIGGTIRISAIWGLVLIITFVA
                     LWPAMWVSPIQTIRTIVVEGWTHYETESKTPRFFNGEIFTSDQFGIKYWYFYPITFLF
                     RSSPVVLFGLILSAWAILKKRHPFESSNIQFVLLSLLLFIGVFTAAMATSDIQHDRYL
                     LPIFAPFDIIAGLGWSSLILSWSRKTTRGYQRYAAPMVALLIIAFQGMLSLSVYPYYF
                     PYYNPLLGGARKAQVILHIGWGEGLDQAAQYLNKKPKAGQLEVVSYYASGCFSYYFNG
                     RTREVSFAKGITDDDWQKFLASDYVVVYINQRQREIASPILDYVADLTPEYTVWINGL
                     DYAQVYKIHE"
     gene            complement(3511..3912)
                     /locus_tag="AC16V7_16170"
     CDS             complement(3511..3912)
                     /locus_tag="AC16V7_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258710.1"
                     /translation="MKLTQMFPKRYATGEDLQGRAITLTIASISCEKMHPQANAPEVE
                     KWVVYFEEAKKGVVLNRTLAYQIAEFLGSEETDDWIGKRITLVPQPMTVAGKKVTAIR
                     ARAVKGAPEALGEAIPAGLVEEDEDGEGIPG"
     gene            complement(3987..4409)
                     /locus_tag="AC16V7_16175"
     CDS             complement(3987..4409)
                     /locus_tag="AC16V7_16175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258711.1"
                     /translation="MSRLLLSLIVATHMLLSATVGCSRIERLPQAAHEAVIDQAKADY
                     AGWLAPPVSFDQLQEISIRRAWRAKDLPTEYPLTDDNPTMWCVELVISGQQMGSRMEA
                     SEIWVVTSRGSHDPPQWIAAALRTFSSLSPYERCGQNP"
     gene            complement(4441..5553)
                     /locus_tag="AC16V7_16180"
     CDS             complement(4441..5553)
                     /locus_tag="AC16V7_16180"
                     /inference="COORDINATES: protein motif:HMM:NF021507.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HIR6258712.1"
                     /translation="MKRVLLLVFSIILLGAATLASVGAGASLTSQTLPSGLAIPTGFE
                     KVTAVYGAQLYRKDYQGGNPDFVQVVDLSLGAKIELLHGSIKEKREGHGAYGGDDPRI
                     LSRSLQQYWDEFTANHANPFCVVNGQFFYMPESPTRLPFPLKVDGQIVSDGFGKQQFP
                     GQKLMLELWRGRADIRQLSKAALYSSTAPNIVAGLAEDARKAINKYVGRTFIGVVDKD
                     GDARYETVLVFTTRSARQVDAAEVLRSFGAQKVMMLDGGGSTQMICMDKSYIASERLI
                     PQALGVQGGVPPSPSNKKAQISSAETSSSAEAEDSPQAKPVQVASEPSEPAQVGASQI
                     ALAQPLYDLGNILWVPGMMLPAMAFILLLVLRTRFN"
     gene            complement(5773..>6064)
                     /locus_tag="AC16V7_16185"
     CDS             complement(5773..>6064)
                     /locus_tag="AC16V7_16185"
                     /inference="COORDINATES: protein motif:HMM:NF016764.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HIR6258713.1"
                     /translation="SYLVGTSLFGGQATLGEMLRVLGFAQAPQLLGIIPCLGGLVGAI
                     WALIASFIAIRQGLDLDNTKAFLTALIGFVVLMIGYAIIYSMLGIGGALLGI"
BASE COUNT         1524 a         1591 c         1636 g         1313 t
ORIGIN      
        1 cagtagaggc cgggagacaa cgcgatagcc aggatcaata tttttgatgc ggaccggagg
       61 aacggagaga gtgcattcgg cttgcggtct ggcgatgaca accttccgag acgttgtgca
      121 taaaggaaaa gtttttccac cccaattcca gcaaagacga acagcggtgg tattatgaac
      181 aaaaactggc ggaagttatc gtacatggtc ggcataacaa ctatggcgcc agcaagcggc
      241 gcaaatagcc atagagctgc cagcgccagt gctgggcgct tctctctgtt tcttcggaag
      301 tccaggatag ccaaagagag ccccagcatg aataagacca gcacaggtac ggtgaattgg
      361 agtgccagca gggtgggcaa atagctgcgc ggcagctcgc tgactggaac atctacccca
      421 gcgaacatca ccttcccctc ccagggaaaa tcagatgcct ctctgaaggc gctcaggtag
      481 ccttgcagtg gcgatgacca caagccaggc caggcgaaat aggtaaccaa gccggcgata
      541 gcaaagtagg ctaccaagga ggggacggcc ttgcgccctg atttccacag gcaatagacc
      601 gccaccaaaa ggccggaggc tggtcctatc gcccggatgg ccgaggcaag ccccagaaaa
      661 agcgccgcca gcagcgtctg cctccgggca gcacctgcca cgatagcccc gagaaaaggt
      721 cgcactcttt cacgccaaac ccgtttagat agcctgggta gcagcaaccc agccaaagcc
      781 agccacagga ggggcacagc catagcataa ccagctaaca gcctgcggta ggcggcttgg
      841 cctttctgga tgtagagctc aacggggatg ctccccgtat tctccgccaa tcggttgaaa
      901 agacgcccga gcaagctgga attgccatcg gcataggctc gcgcaactag gttggcgatc
      961 caatcccgga tgggggcatc cgcgagtagt aatccgaaca gaagcgccag caggaaaata
     1021 caggcaccga tcagcaggta gcgtttccgg ggcgatgcct gccgccagtc agtggacagg
     1081 gtatcagcca ggcttgaaaa atctgcttga tctgccgacg atgaaccacg gcttgcatat
     1141 cctgagcttt gagcggcgct tccgcttaga gacgccatcg gtcgtcccag cgccacgctc
     1201 cccaggaaga aggacataaa agggatgtct ttggggttaa tccaggcgtg accccagagc
     1261 agcggctggg tattaaagag caggacagtt gccaatgcgg ccccctcgct catataatgg
     1321 cggcagatga tgtagaggaa acaaaccccc attaaaaagg agaggaaatg catgaagtgc
     1381 caggcctcaa tgggttgcag gctgccccaa acggcgcgca gcgcacgaga cccgatcagt
     1441 gccagcatga cataagaggg acccttctcg tctacgctta cccgtttgcc gtgggtatat
     1501 gctgccagtg agcgctcggc gtagtcaatg cgcagatgct catctcgcga ttcaccgtaa
     1561 tctgttacca ctatgcaacc gatggcgata ttgataacga tgattactgc tatcgtaaag
     1621 tattggcggg agcgaatttg caagaacaga ttccgggtcg aacgggttaa tattttgggt
     1681 cagctattcg tggattttgt agacctgggc gtaatccaaa ccattgatcc agaccgtgta
     1741 ctctggagtt aagtcggcta cgtaatctag gatgggggag gcaatttcgc gttgccgttg
     1801 gtttatgtag actacgacgt aatccgaagc aagaaacttt tgccaatcat catcggttat
     1861 acccttcgca aacgatacct ctctggttct tccgttaaag tagtacgaga aacatccaga
     1921 ggcataatat gagactacct ccaattggcc cgctttaggt tttttattca ggtattgcgc
     1981 tgcctgatcc agtccttcac cccagccgat gtgtaaaata acctgggctt tgcgcgcgcc
     2041 tcccagcaag gggttatagt aaggaaaata gtagggatag acgctcaatg agagcatgcc
     2101 ctgaaaggcg atgatgagca acgcaaccat gggggctgca taacgctggt aaccgcgtgt
     2161 tgttttcctc gaccaggata aaatcaaaga cgaccaaccc aatccagcga tgatatcaaa
     2221 gggggcaaaa atgggcagca gataccggtc atgctggata tcgctggttg ccatggctgc
     2281 agtgaaaact ccgatgaaga gcagcaagct tagcaggacg aactggatat ttgaagattc
     2341 aaacggatga cgcttcttta aaatggccca ggctgatagg atcagaccaa acaacacaac
     2401 tgggctactg cggaaaagaa aggtgatcgg atagaaatac caatatttga tcccgaactg
     2461 atcgctggta aagatctctc cattaaagaa gcgcggcgtt ttggattctg tttcgtaatg
     2521 agtccagccc tcaacgacaa ttgttctgat cgtttgaata gggctgaccc acatcgcagg
     2581 ccaaagggca acaaaggtga ttatcaagac cagaccccag atcgccgaaa ttcggatggt
     2641 tcccccgatg agccttggaa tcgagcgttc ttgtttgccc aatccatctt tccggagtgc
     2701 aaacagggtc aacaggccaa caactggcac aatcaatatt cctggcgatt tggtcagcaa
     2761 tcccagccca gcggcgatag ccgaaaccag caaatctctg gttcgtcttc cctggagaaa
     2821 tgaaatgatc gctagcaagg agagcagaag catgttgcct aataaaccat ccaagtggag
     2881 cacgcgtgtc aaggctacgt gataaggttc gaaggcaatc agcaggaagg agattaatgc
     2941 cggaaatgga ctaatgatgc ggatagcata acaaaagcta actagtagaa ccaacgtatg
     3001 cccaaggatc atgaagacgc ggctagctac caggaggtcc aacgccgaga cccctttggc
     3061 attttggata tagcgattga attcgttgga gtcgaccctg tcgggaccat catgccgata
     3121 ctgcgggtag cggacgagga acccacctgt gccagcccac atcacggtaa gaccagggtg
     3181 ttcgctaagg tatgtccact ccagtttgcc ctggctgata gccatgtaaa aattcgcaga
     3241 gcgtgccagc cacaggtgtt cgtctggcgt gaccaggcga ttcaaggctt ttagacgagg
     3301 cacagcgatg gcaagaccta ccacgatcag cataatccat tgaagccatg cggtgcgacc
     3361 tttgacggat aattcgctcc agtttttggt tgaccaagcg cttctggggt tcattaggct
     3421 tcattataat ctcagaagtt gcgcgtttgg agaggcaaga tgagatggat cagtgcttcg
     3481 cccagaccgc cctaccagac tccgtgataa ttacccagga atcccctccc catcttcatc
     3541 ctcttctacc aggccggcag gaatggcttc acctaaagcc tcgggagcgc ccttgactgc
     3601 ccgggctcgg atggcagtga ctttcttgcc tgccacggtc attggctgtg ggacgagcgt
     3661 gatccttttc ccgatccaat cgtctgtctc ctcgctgccc aggaattcgg cgatttggta
     3721 agccagcgtg cggttgagca caacgccctt cttcgcctcc tcaaaataca ccacccattt
     3781 ctcaacttcg ggggcgttag cctgggggtg cattttttcg cagctgatac tggcgatagt
     3841 cagggtgatg gctctccctt gcaagtcctc tccggtggcg tagcgttttg ggaacatttg
     3901 tgtaagtttc atgatatgta tcctcatcca agctttcacc agggaacacg cgtctttaaa
     3961 agattatccc aacctgttta ttcgcattaa ggattttgac cacatcgctc atacggtgac
     4021 agagaagaaa aagttctcag tgcagctgct atccactgag gtgggtcgtg gctcccgcgg
     4081 cttgtgacaa cccaaatctc gcttgcctcc atcctggaac ccatctgctg cccggatatc
     4141 accagctcca cgcaccacat ggtagggtta tcatctgtaa ggggatattc tgtgggtagg
     4201 tccttggcgc gccacgcgcg gcggatactg atctcttgga gctggtcaaa gcttactggt
     4261 ggggcaagcc accctgcata gtctgcttta gcctggtcaa taactgcttc gtgagctgct
     4321 tgtggaaggc gctcgatcct tgagcaacca accgttgcac tcagcagcat gtgggtcgcg
     4381 actatcaagg ataataataa tcgcgacatg tctacttttt ccactcttct cggcttgtgg
     4441 ctaattgaat ctcgtcctga gcacaagcag gaggatgaac gccatggcag ggagcatcat
     4501 gcctggaacc cataagatat tccccaagtc gtataatggc tgggcgagcg cgatttggga
     4561 tgcgccaacc tgggcaggct cactcggctc tgatgcgacc tgtactggct tggcttgtgg
     4621 tgaatcttca gcctcagctg agctagacgt ttcagcggaa gagatttgcg ccttcttgtt
     4681 ggacggagat ggcggcacgc ccccttgcac gcccagggct tgggggatca ggcgctctga
     4741 ggcgatgtac gatttgtcca tgcagatcat ctgggtggag ccgcccccgt caagcatcat
     4801 aaccttctgc gccccaaagc tgcgcagcac ctcggccgcg tctacctggc gggcggagcg
     4861 ggtggtgaag accaaaacag tttcgtagcg ggcatcgcca tctttgtcga ccacgccgat
     4921 aaaggttctc ccgacatact tgttgatcgc tttgcgagca tcctcagcca ggccagcgac
     4981 aatgtttggg gcagtcgagc tatacagagc tgctttagag agctggcgga tatcggcccg
     5041 accacgccaa agctccagca tcagcttttg accaggaaat tgctgctttc caaagccatc
     5101 gctaacgatc tgtccgtcca ccttgagagg gaacggcaaa cgcgtagggc tttctggcat
     5161 gtagaagaac tgcccattga ccacgcaaaa cgggttggcg tggtttgcgg taaattcatc
     5221 ccagtattgc tgcagtgaac gggaaagaat gcgcgggtcg tcacccccat aagcgccgtg
     5281 tccctcccgc ttctccttga tcgacccgtg gagtagctcg attttcgccc ccagggatag
     5341 gtcaaccacc tggacgaagt cggggttacc gccctggtaa tccttacggt agagctgggc
     5401 gccatagacg gcggtgacct tctcaaaacc cgtggggata gctagaccgc tgggaagggt
     5461 ttgagaggtt agcgaagctc ccgccccaac ggatgctaaa gtcgctgcgc cgagcaggat
     5521 aatcgagaag acaagcaaga gtacgcgttt cattgatggt tgcaaacatt gggtgcaaat
     5581 gatgatgcgt gggatgtaaa atattgcacc ggcaaaatca ttttagcaaa tattttcaat
     5641 aaattgtagc acatacgttc taatttgaga aggaaaaata tctggtttct acgtgcctat
     5701 cgaaaggcgc gcagaaacca gagtatcgaa aatatcttca atcgctcata atgatgagtt
     5761 ctgaaggata aattatatcc ccagcaatgc cccaccgatg ccgagcatcg aatagattat
     5821 agcgtacccg atcatcagaa ctacgaatcc tatcagagca gtgaggaatg ccttggtgtt
     5881 atccaggtct aagccttggc ggatggcgat gaaactggcg atcaatgccc aaatggctcc
     5941 caccaaccct ccgagacagg ggataatccc caagagctgc ggagcctggg cgaagcctag
     6001 cacacgtagc atctcaccga gtgtggcctg tccaccgaac aggcttgtcc ctaccaggta
     6061 ggac
//
LOCUS       DBNHOH010000896         3250 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_441762, whole genome shotgun sequence.
ACCESSION   DBNHOH010000896 DBNHOH010000000
VERSION     DBNHOH010000896.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_441762"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..869
                     /locus_tag="AC16V7_16190"
     CDS             141..869
                     /locus_tag="AC16V7_16190"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR6258714.1"
                     /translation="MIPLRDTIRSRSFPLVNLGIIGLNTLVFLFELNLSPRGLNQFIF
                     TFGMVPARLNLTTPLALLDNPLPLITLFSHMFMHGGWVHFLSNMWTLFIFGDNVEDRM
                     GGGRYFVFYILAGLASGLLQALVAPGSSVPAIGASGAIAGVLGAYFILYPGGRVLTLI
                     PIFIFPWFVEIPAIIYLGFWFVSQLFSGLAALNMPAEASMGGVAWWAHIGGFIFGILA
                     YRFFVRRIQPIYYRQFPRFPEDSF"
     gene            888..1760
                     /locus_tag="AC16V7_16195"
     CDS             888..1760
                     /locus_tag="AC16V7_16195"
                     /inference="COORDINATES: protein motif:HMM:NF024245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HIR6258715.1"
                     /translation="MMDDQVTQVDLNTADMDELRTLPGVGPALAERLLQARPFASIED
                     LGRVNGFGPAALERLQPHVTLSKVETSSLATDTEEELAEHEPTASVVDEISPEAEEGG
                     ASMLEPAGEEISPQMEAEMPEAPQTPKPQPASQPVTAPKAGPRPASRMEAFWLACGAL
                     VFATLLGAALSLGFLALINGGLSYARPSQINQLARQIDGTKAQTGILEQDMDTLRARI
                     DNLEGLSGRVGTVEKDMEGLREDMDAALSSMDALSLQVENLDGQVAEIEARTERFQGF
                     LEGLQELLGGLFSP"
     gene            1805..2722
                     /locus_tag="AC16V7_16200"
     CDS             1805..2722
                     /locus_tag="AC16V7_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258716.1"
                     /translation="MSDQSPSPPPPSFGNRLGLAFISFLRALVRVLLILLLVALLGAI
                     VYFGVPALYQRYVQPVERDLARLNDFQARQEQDDLQFNRRLDELQERLTEIELQNDTQ
                     KQDLDELQTRLEDMESERQFGTQAGESAQATAIARLDEMETYLAELSTDLEQMQAEIT
                     ALNQALAKNSAQIQELSSRTDSGAAPLEALRIDLQLVKAMEHLTRSRLFLAQNNLGLA
                     EQDVRDARQLLTNLQGQVPAFQIDALARIIARLDAALSSLPSRPFLAAEDLEVAWQLL
                     QGGLPGEPTTQTTSTPASIQGTPTPSATP"
     gene            2748..>3250
                     /locus_tag="AC16V7_16205"
     CDS             2748..>3250
                     /locus_tag="AC16V7_16205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258717.1"
                     /translation="MDDEKQNEKPASAIPEATKEKVSPPPSRLNRIALRLLRWLLGIL
                     IVMGLGALLAIYTLYMPTRQKLMDSQQQVEQADQRVAELEGEVGNLSTFEEQNQDLLD
                     ELEKTTLHVTILSARADVAAAQLALAQNDPAKARLALSKTPQTLKNLESMLEPGKKKL
                     AKDMQARL"
BASE COUNT          705 a          869 c          917 g          759 t
ORIGIN      
        1 cagcctaaaa attatctttt accctaatgt gacgtttcgg cgagaatgtt ctcgccgaaa
       61 cgtcgttatt taacagaaat acccttttcc ggttatactt tagttagccc tgggggtagt
      121 gcccccagcg aacagtttac atgatccctt taagagatac catccgctcc cggtcgtttc
      181 ccctggttaa tttggggatc atcggcctga ataccctggt ctttcttttt gagctgaacc
      241 tctcgcctcg agggctgaac caattcatat ttacatttgg tatggttcca gcccgcttga
      301 atctcactac ccctctggcg ttgctggata acccgctgcc tcttatcacc ctgttctcac
      361 acatgtttat gcacggtggg tgggtgcatt ttctcagcaa tatgtggacc ttgtttattt
      421 tcggcgataa cgtggaagac cgcatgggcg gaggacgtta tttcgtgttt tatatcctcg
      481 ccggtttagc ctccgggctg ttgcaggcgc tggttgcgcc cggtagcagc gtccctgcca
      541 ttggcgcaag tggagcaatc gccggcgtct tgggtgcgta tttcatcctt tacccgggcg
      601 ggcgggtctt gactctgatc cccatcttta tttttccctg gttcgtcgag atcccagcca
      661 ttatctatct ggggttctgg tttgtatccc agcttttttc gggcttggct gcattgaaca
      721 tgcctgccga agccagcatg ggaggggttg cctggtgggc ccatattggc ggatttatat
      781 tcgggattct ggcctatcgt ttttttgtaa ggcgtatcca gccgatctat tatcgccagt
      841 tcccgcgctt tcctgaagat tccttctaga cattgagcag gaggtcaatg atggatgatc
      901 aagtaactca ggtagacctg aacactgcag atatggatga attgagaacc ttgccgggtg
      961 ttggtccggc cctagcggag cgcctcttgc aggcacgccc gttcgcttca attgaggacc
     1021 tggggcgagt gaacggcttt ggcccagcgg cacttgaacg cttgcagcct catgtgaccc
     1081 tttctaaggt ggaaacttcc agccttgcca cagatacaga agaggagctg gcagagcacg
     1141 agccaaccgc ctcggtggtg gatgagattt caccggaagc ggaagaaggc ggcgcgagta
     1201 tgttggaacc agctggcgag gaaatatccc cccaaatgga ggcagagatg ccagaagcgc
     1261 cacaaactcc taagccgcag ccggcttccc aaccggtcac cgctcccaag gcaggaccgc
     1321 gtcctgccag ccgcatggag gctttttggt tggcttgcgg cgctttggtc tttgcaaccc
     1381 tgctaggcgc tgcactgagc ctgggctttt tggccctgat caacggcggg ttgagttacg
     1441 ccagaccttc ccagatcaac cagctcgcca ggcaaatcga tggtaccaaa gcccagacgg
     1501 gaattttgga gcaggatatg gacaccttgc gcgcccgcat tgataacctt gagggcttga
     1561 gtgggcgcgt cggtacggtt gagaaagata tggaaggttt gcgtgaagat atggacgccg
     1621 cgctgtccag tatggatgcg ctgagccttc aggtggagaa cctggatggc caggtggccg
     1681 agatagaggc gcgcacagag cgctttcagg gattcttgga agggttgcaa gagcttttgg
     1741 gtggcttgtt ctcaccctga gcgctttgga taccctataa cgtcttattc aagaaggagg
     1801 tctcatgtca gatcaatctc cctcgccccc accaccttcg tttggaaacc gtctggggct
     1861 ggccttcatt agtttcttgc gtgctttggt acgcgttctg ctgattctct tgctggttgc
     1921 gttgctggga gcgatagttt atttcggtgt tccagccctt taccagcgct atgtgcagcc
     1981 ggtagagaga gatctggccc gcctgaacga tttccaagcc cgccaggaac aggacgatct
     2041 tcaattcaac cgccggctag atgagcttca ggaacgcctg acggaaatcg agctgcagaa
     2101 cgatacccaa aagcaggatc tggatgaatt gcaaacccgg cttgaagata tggagagcga
     2161 aaggcaattt ggcacccagg ccggagagag cgcccaggcg actgctatag ctcgtctgga
     2221 tgagatggag acatacctgg ctgagttgag cactgacctg gagcaaatgc aggcggagat
     2281 taccgccttg aaccaggctt tagcaaagaa cagtgctcaa atccaggagt tgagcagccg
     2341 gaccgattcg ggcgccgccc ccttggaggc actgcgtatc gatttacaat tggttaaggc
     2401 gatggaacac ctgacgcgca gccgcttgtt cctggcacag aataacctgg gtttggctga
     2461 gcaggacgtg cgagatgcgc ggcagttgtt gaccaacctg cagggccagg tacctgcttt
     2521 ccagatcgac gccctggcaa gaatcatagc ccgcttggac gccgcactga gcagcctgcc
     2581 ctccaggcct ttcttggccg ccgaagacct ggaggtcgcc tggcagttgc tccaaggtgg
     2641 tttacctggg gagcctacta cgcagactac ctccacgccg gcatccattc aaggcactcc
     2701 aacaccttcc gctacgccct aatatacaac ctttggagaa gaacaccgtg gacgacgaga
     2761 agcaaaatga aaaaccggct tcagccatac ctgaagcgac gaaggaaaag gtcagtccgc
     2821 cacctagccg cttgaaccgc atcgcattgc gcctattgcg atggctgctg ggcatattga
     2881 tcgtcatggg gctgggtgcc ctgctggcta tttacactct ctacatgccc acccggcaga
     2941 agctgatgga tagccagcag caagtcgagc aagctgacca gcgtgtcgct gagttggaag
     3001 gcgaggtggg gaacctttcg acgtttgaag agcagaacca ggacctgcta gatgagctgg
     3061 aaaaaaccac cttgcatgta actatcctga gcgctcgcgc ggatgtggcc gcagcacaat
     3121 tggccttggc gcagaatgac ccggccaaag cgcgcctggc cttgagcaag acgccacaga
     3181 cgctaaaaaa tctagagagt atgctggaac ccggtaaaaa gaagctggct aaagatatgc
     3241 aagctcgcct
//
LOCUS       DBNHOH010000897         2431 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1403746, whole genome shotgun sequence.
ACCESSION   DBNHOH010000897 DBNHOH010000000
VERSION     DBNHOH010000897.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1403746"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="AC16V7_16210"
     CDS             <1..983
                     /locus_tag="AC16V7_16210"
                     /inference="COORDINATES: protein motif:HMM:NF024891.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HIR6258718.1"
                     /translation="YKVGYLKPFSLQPWRVSGHVADEDAAFVIEALGLEAQPWELSPV
                     VVTSEALRQRLHQPQPEDLLDRVKAVAQSIGANVDMLLVEGGASLREGYIFDLSTPRV
                     AEALGSPVLVLIKYQDPVRLMDDALTATFRLGKLLVGAIINRVPDEARQFVTQDAVPA
                     LERLGVPVFGVLPERRGLEALTVEELIEVLNPQVLAAGSDPQALVETLTVGAMTADAA
                     LSRFRRQMNKAVITGGDRTDIQLAALETSTTCLILTGNLQPSPLIVKQAEEFGVAVLL
                     VPTNTMETIEAIEKVFGKTHLAQPSKLEHFQELVAANVDMARLYKAIGLT"
     gene            1006..1929
                     /locus_tag="AC16V7_16215"
     CDS             1006..1929
                     /locus_tag="AC16V7_16215"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6258719.1"
                     /translation="MLRRSDPRWAYVLVNLATLLWASNIVLGRFLRHEIGPLSLSAAR
                     FSVAGLIFLLLLRKTQKNQALIDQPLGSNPRQPPRMEWLLYLGMSLVGVFGFTTLLYF
                     ALRYTTASHAALINAATPLVTAVLAAVFLKEQFTRPLIVGSILSLAGVGLVIGVGSTN
                     ITGSPQALFGDLLCLVAAVLWGIYSILGRLATKTKSSLQVTAFSIWFALPLLYLAAGV
                     EWQTTPPSLTSPVLLAAVYIGVFPSVIAFSAWNEGVRMAGPNQAMAFYNMLPVYGTLL
                     GVLLLGESLSWNALFGGILVIAGGIMIARLR"
     gene            1965..>2431
                     /locus_tag="AC16V7_16220"
     CDS             1965..>2431
                     /locus_tag="AC16V7_16220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6258720.1"
                     /translation="MSKQSPLLYSQLADWFHLLTRPEDYAEEAEFYRRTILSNSPLPP
                     STLLELGSGGGNNASHMKAHFQLTLVDISPHMLSISRSINPECKHILGDMRTIRLSRV
                     YDAVFIHDAISYLTTLSDLEQAIRTAWIHCRTRGVALFAPDFTRETFQAGTSHG"
BASE COUNT          536 a          680 c          621 g          594 t
ORIGIN      
        1 gatataaggt cggctattta aaacccttca gtctgcaacc ctggcgagta agcggtcatg
       61 tagctgacga agacgccgca ttcgtaatcg aggctttagg ccttgaagca caaccctggg
      121 agctctctcc agtggtagta acgagcgagg cgttaaggca acgacttcac cagcctcaac
      181 cggaagatct tctcgaccgc gtcaaagctg ttgcgcaatc cattggcgca aacgtcgaca
      241 tgcttctcgt tgaagggggc gctagcctgc gggaaggata catctttgac ctttcaaccc
      301 cgcgggtggc tgaggcgctg ggcagtccgg tattagtgtt aatcaaatac caggatccag
      361 tgcgcttgat ggatgacgct cttacagcga ccttccgtct gggaaagttg ctggttgggg
      421 cgattatcaa ccgcgttcca gacgaagcca ggcagtttgt cactcaggac gcagtacctg
      481 ccctcgagag acttggcgtg ccggtcttcg gggttttacc cgagcgacgt ggcctggaag
      541 cattgacagt tgaggaactt atcgaggttt tgaaccctca agtgctggct gctggatctg
      601 atcctcaagc attggtcgag acattaaccg tgggggcaat gaccgctgat gccgccttga
      661 gccgcttccg cagacaaatg aataaagcgg tcatcactgg cggtgatcgt accgacatcc
      721 aactggccgc cctggagaca tccaccacgt gtctgatatt gaccggcaac ctgcaaccca
      781 gcccgttaat cgtcaagcaa gccgaagagt tcggcgtagc ggttttactg gtacccacca
      841 acactatgga gacaatcgag gcgattgaga aagtatttgg taaaactcac ctagctcagc
      901 catccaagct ggagcatttc caggagctgg tggcagccaa tgtagacatg gctaggcttt
      961 ataaagcaat tggcttgacc tagagaatta atttcctctc gaacaatgtt gcgtcgcagt
     1021 gaccctcgct gggcatatgt tctggtaaac cttgccacgc tactctgggc aagcaatatt
     1081 gttctcggac ggtttctccg tcatgagatt ggccccctct ctctctccgc ggcacgcttc
     1141 agtgtcgccg ggttgatttt tctcctcctg ctaagaaaga cacaaaaaaa tcaggctctt
     1201 attgaccagc cgctgggctc aaatccgcgc cagccacccc ggatggaatg gctcctatac
     1261 cttgggatga gtttagttgg tgtattcggt tttacgactt tgctttactt cgccttgcgc
     1321 tataccaccg ccagccacgc agcactgatc aatgcagcta ccccattggt tacagcagta
     1381 ttggcagcag tatttttaaa agagcaattt acccgtcccc tcatcgtcgg gagcatatta
     1441 tccctagctg gcgtaggttt agtcattgga gttgggtcaa ccaatatcac tggatctcca
     1501 caagccttgt ttggtgactt gctctgtctg gtggcagctg tactctgggg tatctattca
     1561 atcttaggcc ggctggcgac aaaaaccaaa tccagcctgc aggttacagc attttccatc
     1621 tggttcgcct tgcctttgct ctatctggct gccggagttg aatggcaaac aacacctcct
     1681 tcactgacct ctcctgtact actcgcagcc gtgtatatcg gcgtctttcc atctgtgatc
     1741 gctttttccg cctggaacga gggggtgcgc atggcagggc caaatcaagc catggcgttt
     1801 tataacatgc tccccgtata tggcacgctg ctcggggtat tgctcctggg agagtctctg
     1861 agttggaatg ccctgttcgg cggcatactg gtcattgccg ggggcatcat gatcgcaaga
     1921 ttgcgttagc atcttccaca cctgcactag gagcaagctg ctaattgtcg aagcaatccc
     1981 cactcttata cagccagctc gcggattggt ttcatctgct tacccgccca gaggattacg
     2041 ccgaggaagc tgaattctac cggcgcacca tcctgtctaa ctcacctctg ccgccgagca
     2101 cacttcttga gctcggaagc gggggcggca ataatgcctc ccacatgaag gcacacttcc
     2161 agctcacttt ggtggatatc tctccacaca tgctctctat aagccgctcg attaaccccg
     2221 aatgtaagca tatcctgggt gacatgcgga caatcaggct cagccgcgtc tacgatgcgg
     2281 tcttcatcca tgacgcgatc agttatctca ccacgctcag cgacctcgaa caggctatcc
     2341 ggacggcatg gatccactgc cggactcgag gtgtggcgct cttcgcaccc gatttcactc
     2401 gagagacgtt tcaagcaggc acaagccacg g
//
LOCUS       DBNHOH010000898         3447 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_634793, whole genome shotgun sequence.
ACCESSION   DBNHOH010000898 DBNHOH010000000
VERSION     DBNHOH010000898.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_634793"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="AC16V7_16225"
     CDS             <1..461
                     /locus_tag="AC16V7_16225"
                     /inference="COORDINATES: protein motif:HMM:NF024585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR6258721.1"
                     /translation="MVLGQIPFFPAQRPAYRLEELKPGGETFLDLYPEILRCFGCNTC
                     TKSCPQDLDVMSYMSAALRGDLQALADLSFDCIMCGLCVARCPAELVPPNVALLGRRL
                     YGKYLAPTADHLHRRVQEMESGIYLDAISELKNLPLADLKKRYNTREIEA"
     gene            491..1621
                     /locus_tag="AC16V7_16230"
     CDS             491..1621
                     /locus_tag="AC16V7_16230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR6258722.1"
                     /translation="MDFEFSSDTLMLRDMLRRFVQKEARPLEMKYFTAGVLAPEEKAR
                     LHRSIEQMGLWGLTAPEQFGGGGLDLVNCCVIQEELGQTFIPVEIGEVTPLLFSCSGE
                     QVSGYLEPALAGERRAILAAREPGSCRPDNWTTHASVVEGGPSSGFRLNGNKVIGVQP
                     DPADFFIVIANGHEQRFTAFLIDGDHPGLEVSANGEFTLALRDCEVGKEAVLGEPGGA
                     FGLGVQEAPRAWIQTGARTVGIVQRLMEMAVEYARDWESLGAPLAIRPAIQRMIAEIS
                     VELESSRWLVYHAAWLADKGETDETRFQAAQVRLATAEMLKRAVDRVTMIYAGPGPST
                     QIEPQRLIRSVVPMDALELTLENARAAISTQVLGLQNLGGDS"
     gene            1618..2691
                     /locus_tag="AC16V7_16235"
     CDS             1618..2691
                     /locus_tag="AC16V7_16235"
                     /inference="COORDINATES: protein motif:HMM:NF016328.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR6258723.1"
                     /translation="MNVYRVLEVRNGDVVSTLREFMAEWWDYYQPDVLLAPVESADRR
                     GFRPKVINNPAELAGINPFAPVMQENAALAASRLIHDHAGEQVAVMLRPCELRAFVEL
                     RKRQREMESQDNVTVIGVDCLGTFTQADYRRHIDLRGPDDVTAEVLSNAAEGGLRPQR
                     FRTACQICDWPAPRGADMTIGTIGVASNKHLLLIANDEKTDSNLGLNSLTDHLANEYQ
                     ASRRETVVGAIADARTGLRKSMIEDMQGVCRFDDLGCILAWFANCSLCGECLKACPIY
                     DNEFADLVNQAGALKVDRPPLKDLVSLSRWLASCAGCGMCEEECAMDVPLFLLISALS
                     HRIREKMHYRSGDPAQRLPWVSG"
     gene            3004..3267
                     /locus_tag="AC16V7_16240"
                     /pseudo
     CDS             3004..3267
                     /locus_tag="AC16V7_16240"
                     /inference="COORDINATES: protein motif:HMM:NF013086.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
BASE COUNT          727 a          874 c         1050 g          796 t
ORIGIN      
        1 gcatggttct tgggcagatc ccatttttcc ctgcccaacg cccggcttat aggctggaag
       61 aactcaagcc cgggggtgaa actttcctcg atctttaccc ggaaatactg cgttgtttcg
      121 ggtgcaatac ctgtacaaag tcctgcccgc aggacctgga tgtgatgagc tacatgtccg
      181 cggccttgcg cggagatcta caagctctgg ctgatctctc cttcgactgc atcatgtgtg
      241 gcctgtgtgt agcccgctgt ccggctgagc tggtgccgcc aaatgtagcc ctgttgggtc
      301 gtagactgta tgggaagtac ctggccccaa cagcagatca tctgcatcgg cgcgtgcaag
      361 aaatggagtc ggggatatat ctggatgcta tttctgagct taaaaatttg ccgttagcag
      421 atctgaagaa acggtataac acccgggaaa ttgaagcata gaccgattgg tttgaggagt
      481 gaggtaacca atggattttg aattttcatc cgataccctc atgctccgcg atatgctgcg
      541 ccgttttgtg cagaaagagg cccggccgtt ggagatgaaa tactttactg ccggggtcct
      601 ggcacccgaa gaaaaagccc gcctgcaccg ttcgatcgaa cagatggggc tgtggggtct
      661 gactgccccc gagcagtttg ggggtggcgg gctagacttg gtgaattgct gcgttatcca
      721 ggaggagctt ggtcagacgt ttatcccggt tgagattggc gaggtgacac ccctgctgtt
      781 ctcttgtagc ggtgaacaag tctctggcta cctcgagcct gcactggcgg gtgagcggag
      841 ggccattctg gccgcgcgtg agcccggaag ttgccgccct gataactgga caacccatgc
      901 ttcggttgta gaaggcgggc cttccagtgg tttccggctt aacggcaaca aggtcatcgg
      961 agtgcagccc gacccggccg atttctttat cgtcatcgcc aacggtcacg agcaacgttt
     1021 cactgctttc ctcatcgatg gcgaccaccc tggactcgag gtttctgcga atggtgagtt
     1081 cactcttgcc ctgcgagatt gtgaggtagg caaagaagcc gttctgggcg aaccgggagg
     1141 ggcatttggc ctcggggtgc aagaagcacc gcgcgcgtgg attcaaacgg gtgcccgcac
     1201 cgtgggtatc gtgcaacgct tgatggagat ggcagtggaa tatgccagag attgggagtc
     1261 tctcggcgcg ccgctggcaa ttcgtccggc gatacagcgc atgatagcag agatcagcgt
     1321 ggagctggag agctctcgct ggctggttta ccatgccgcc tggctagctg acaagggaga
     1381 gacggatgaa acgcggtttc aagccgctca ggtccgcttg gcaactgccg aaatgctcaa
     1441 gcgcgcagtg gatcgggtga ccatgatcta tgcaggtccg gggccttcta cccaaattga
     1501 gccgcagcgg cttatccgca gtgttgttcc tatggacgcg ctggaactaa ccttagaaaa
     1561 tgcccgcgca gcgatttcaa cccaggtatt aggccttcaa aacttaggag gtgactcatg
     1621 aacgtatatc gcgttcttga ggtaaggaac ggggatgtgg ttagcacatt gagagagttc
     1681 atggctgaat ggtgggatta ttaccaacca gatgtgctgc tggcgccggt tgaatctgct
     1741 gatcggcgtg gcttccgccc gaaggtgatc aataaccccg cggaactggc tggaataaat
     1801 ccctttgccc cagtgatgca ggagaatgca gccctcgctg ccagccgttt gatccatgac
     1861 catgctggtg agcaggtggc agtcatgttg cgcccgtgtg aactgcgcgc ttttgtggag
     1921 cttagaaaac ggcagagaga aatggaaagc caggataacg tgaccgtcat cggggttgat
     1981 tgcttgggca cgttcaccca ggcggattac agacgccata tcgacctgcg cgggcctgat
     2041 gatgtcaccg ctgaggtgtt gagcaacgcc gctgagggtg gcttgcggcc tcagcgcttt
     2101 cgcacagcct gccagatctg cgattggcca gctccgcgcg gcgctgatat gaccatcgga
     2161 acaatcggtg tcgcctccaa caaacatctc ttgcttattg ccaacgatga gaagacggat
     2221 tcgaacctgg gattgaatag cctgacagat catttagcta acgaatacca ggcctctcgc
     2281 cgggagactg tggttggtgc gattgctgat gcacggaccg gtttaagaaa aagtatgatc
     2341 gaagatatgc aaggtgtctg ccggtttgac gacttgggct gcatactagc ctggtttgcc
     2401 aattgttcgt tgtgcggcga atgcctaaaa gcctgtccaa tctatgataa cgagtttgcc
     2461 gatttggtga atcaagctgg cgcgctcaag gtggatcgcc cgccattgaa agatctggtc
     2521 agtttaagcc gctggctggc ttcatgtgcc ggttgcggca tgtgtgaaga ggagtgcgct
     2581 atggatgtgc ccctctttct gctgatctca gccctcagtc atcgcatacg cgagaagatg
     2641 cattaccgaa gcggtgatcc tgctcagcgg ctcccctggg tctcggggta aaacccgtcg
     2701 agttgcgcaa tctaattgtc ctattaatgg gaggtggaaa tgtcctatcc ggccataatt
     2761 cgtgaatcga tgcagaaagt tgaagctagc cgggcacgcc gcgcccagga aactcatccg
     2821 cgctttagtc cgcaggagaa ggaagagatc ttgcgtaaat tccatccaga ctatatcgcc
     2881 gaggcttttc gggggctgcg tctgggtccc aataaaggcg atcgcgcccc gcgcgaattg
     2941 gtcgatgtgc tggaagcacc ttctctcctg tcatcaagtt atgatctgga ccgggagcct
     3001 gacatgacct gcgacctgct ggtcattggg ggcgggggag ctggggccag cgcggctctg
     3061 ctggcgcagg agaatggtgc tgaggttctg gtgactacca agctgcgttt tggggatgcc
     3121 aacaccatga tggctcaagg aggcatccag gcggcagata aagacaatga ttcgcccgcc
     3181 atgcactatc tggacgtatt gggaggtggg gcttttagca atgaccccga tctagtggaa
     3241 gctttggtga cagatgcacc catggtcatt gcctggcttg aatcgttggg ggtcatgttc
     3301 gataaagagc cagatggcgt cctgcgcacc aaggccgcgc accttttccg tcaccagctg
     3361 ccccaggatt tgctctggat aggctgctcc ggtgggatgg tactgcatgg tatcgatgaa
     3421 agacaacttg gctcccaggc gatatgc
//
LOCUS       DBNHOH010000899         3312 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1595644, whole genome shotgun sequence.
ACCESSION   DBNHOH010000899 DBNHOH010000000
VERSION     DBNHOH010000899.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1595644"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..306
                     /locus_tag="AC16V7_16245"
     CDS             49..306
                     /locus_tag="AC16V7_16245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR6258724.1"
                     /translation="MPTLNRVQLIGRLGRDPEVRHTPSGKKVTSFSLAVDRRWRSSEG
                     EIKSSPSGTNIKNALEICGTSRASLPECGECLVLLRETKLF"
     gene            complement(450..2228)
                     /locus_tag="AC16V7_16250"
     CDS             complement(450..2228)
                     /locus_tag="AC16V7_16250"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HIR6258725.1"
                     /translation="MTLPYTQTRWSLSDLFPALDSPELEQTFTDLETQVANFESVRSQ
                     LSSQIKQAEFMNFVTLLEEINQLGSRLYAFAGLSFSADTQDQAAQTLVARVDQFIAIL
                     QNRTLFFSLWWKDLDDDNAARLMSEAGDYRYWLEEMRHFKPHTLTEPEEKIINIKNVT
                     GVSALGNLYDSITNRYTFKLQVDGEVKELTRGELMVYVRQHDADLRAAAYQELYRVYG
                     EEGPILGQIYQTLVRDWRNEQVDLRRFSSPISARNLMNDIPDPVVNTLLDVCERNASL
                     YQRYFRLKAKWLGMDRLRRYDIYAPVVKSDKRYEYTQAAEMVLGSFHEFEPRLAGLAR
                     RVFAENHLDSEVRKGKRGGAFCMSVTPKMTPYVLLNYQGRADDVATMAHELGHAIHTM
                     LAADHSMFTFHASLPLAETASTFGEMMLIDRLLAQEDDESVRRDLLFRQVDDSYATIM
                     RQAYFALFERDAHEMVDQNASADELSAAYMENLKRQFGEVVEISDEFRWEWVSIPHIY
                     QVPFYVYAYAFGQLLVLSLYKQYKVEGESFKPRYVSLLSSGGSEAPERLLSQAGIDIR
                     SADFWQGGFDVLSGLLDQLEEIPIDA"
     gene            complement(2301..>3312)
                     /gene="ychF"
                     /locus_tag="AC16V7_16255"
     CDS             complement(2301..>3312)
                     /gene="ychF"
                     /locus_tag="AC16V7_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIR6258726.1"
                     /translation="TRGNAPTTMSGGRFEVHTAVVDVPDQRVDRLSAIFRPQKTVYAK
                     VTYADIAGLEAGASKAGISGTLLNQLTQMDGFVHVVRCFQEASVPHPSGSVDPQRDID
                     AMDAELLLNDLIAVERKLERLGDERRKGGGRDKAVIERELALFEKLHDALGQEKPLRE
                     VEFSAEEQKSLSGFGFLTRKPVLLVLNLSDEQTPPDVAYPHAHTAVMPLQGRLEMELA
                     QLSPEDAEVFMGEYGIQELSLYKVIRLSYDLLGLQSFFTIGSDEVHAWTICRGDNAHS
                     AAGVVHTDLQKGFIRAEVISFDDLVALGGLSEARAKGKLRLEGKDYILQDGEIMHVRF
                     NV"
BASE COUNT          737 a          929 c          924 g          722 t
ORIGIN      
        1 gcaaacccgc gctaaactgg agaaagacac catctaagga ggaaagacat gcctactcta
       61 aaccgcgtac agttgatcgg acgtttgggc agagacccgg aggtgcgaca tacaccttcg
      121 ggcaagaagg tcaccagctt cagcctggcg gtcgatcgcc gttggcgcag cagcgaaggg
      181 gagataaaat catcacccag cggtactaat ataaaaaatg ccctggagat ctgtggaacc
      241 tccagggcat ctctccctga atgcggcgag tgtctcgtat tactcaggga gactaaattg
      301 ttctgattta gctgcaagat ttgtgccaac cacaaaccga aaatatatca acaagtatca
      361 tgttttgata catggaacgt tggatttgtc cagcagggct tatacccgat cttaggctgg
      421 ggctgctttg acagaagatt cacgtttggt tacgcgtcga tgggtatctc ttcgagctgg
      481 tccagcaagc cagagagcac atcgaagccc ccttgccaga aatccgcact gcggatatca
      541 atccctgcct gggagagcaa gcgctctggg gcttcggaac caccagaaga aagcaggctg
      601 acatagcgtg gcttgaagga ttcgccctcc actttgtact gtttgtacag ggatagcact
      661 aggagctgac caaaggcata ggcgtacaca tagaacggta cctgatagat gtgcggaatg
      721 gaaacccact cccagcggaa ttcgtcgctg atctcgacca cctcgccgaa ctgacgcttc
      781 aagttctcca tataggctgc tgagagctca tccgccgaag cgttttgatc taccatctcg
      841 tgggcatccc gctcaaacag tgcgaagtat gcctggcgca tgatcgtggc gtaggaatcg
      901 tccacctggc ggaagagcag gtcacggcgg acgctctcgt catcttcctg tgccagcaag
      961 cgatcgatga gcatcatctc gccaaaagta gaagccgttt ccgctagggg cagcgaggcg
     1021 tggaaggtga acatgctgtg gtcggctgcc agcatggtgt ggatggcatg tcccaactca
     1081 tgcgccatgg tggcgacatc atctgctctg ccctgatagt tgagcaacac ataaggtgtc
     1141 attttgggcg tcaccgacat gcagaaagcc ccgccgcgct tgccctttcg cacctcgcta
     1201 tccaggtgat tttcagcgaa gacgcgcctg gccaagcctg ccagcctggg ctcaaattcg
     1261 tggaatgagc ccagcaccat ctcggccgcc tgggtatact cgtaacgctt atccgatttg
     1321 accaccggcg cgtagatatc gtatcggcgc aggcgatcca ttcccagcca ttttgccttg
     1381 aggcggaaat agcgttggta cagggatgca ttgcgctcac aaacatccaa cagggtatta
     1441 actaccggat cgggaatatc gttcatcagg ttgcgggcgc tgatcgggct gctgaagcgg
     1501 cgcaggtcca cctgctcgtt gcgccagtcg cgcaccaaag tctggtatat ctgccccaag
     1561 attggaccct cttccccgta aacgcggtag agctcctggt aggcggcggc acgcaggtcg
     1621 gcatcgtgtt gtcggacgta taccatcaac tccccgcggg tcagttcctt gacttcgccg
     1681 tccacctgca gcttgaaagt atagcggttg gtgatcgagt catacaggtt gcccagtgcg
     1741 cttacaccag tgacattctt gatattgatg atcttctctt ctggctcggt gagggtatgc
     1801 ggcttgaagt ggcgcatctc ctccagccag tatcggtaat cgccagcttc agacatcagc
     1861 cgcgccgcgt tgtcgtcgtc cagatccttc caccacaggc tgaagaagag cgtgcggttc
     1921 tgcaggattg ctataaattg gtccacgcgg gctaccaatg tctgggctgc ctgatcctgg
     1981 gtatcggcgc tgaagctcaa tccagcaaag gcatacaggc gcgatcccaa ctggtttatc
     2041 tcttccagta gggtgacaaa gttcataaat tcggcttgct tgatctgtga ggacaactgc
     2101 gagcgcacgg actcaaaatt ggcaacctgg gtttccaggt cggtaaaggt ctgctccagc
     2161 tccgggctat ccagagctgg aaaaaggtcg ctaaggctcc agcgggtctg ggtgtagggt
     2221 aaggtcatgg ggatctctct ttgatatgaa tatatatagt caagatggcg gagagtatta
     2281 tacccgaagg gctcattggc tcacacattg aacctgacgt gcatgatctc gccatcctga
     2341 aggatatagt ctttgccctc caggcgcagc ttgcctttgg cgcgggcctc gcttaaccct
     2401 cccagcgcaa ctagatcatc aaaggaaatc acctcggcgc ggatgaagcc tttctgcaga
     2461 tcggtatgga cgacgcccgc tgcgctgtgg gcgttgtcgc cccggcagat ggtccaggcg
     2521 tgcacctcgt ctgatccaat ggtaaagaaa gactgtaagc ccaacagatc gtatgacagg
     2581 cggatcacct tgtacaggct cagttcctgg atgccgtact cccccataaa gacctcggcg
     2641 tcctccggcg agagctgtgc cagctccatc tccaagcgtc cttgcagcgg catgactgcc
     2701 gtatgagcat gagggtaagc cacatccggc ggcgtctgct cgtccgagag gttcagcacc
     2761 aacagcactg gtttgcgggt caggaagcca aaacccgaga gagacttttg ctcttcggcg
     2821 gagaactcaa cttcccgcaa gggcttctcc tgtccgaggg cgtcgtgcag cttttcaaaa
     2881 agggctaact cgcgctcgat caccgcctta tcacgtcccc cgcccttacg ccgctcgtca
     2941 cccaggcgct ccagcttgcg ctccacagcg atcagatcgt tgagcagaag ctccgcatcc
     3001 atggcgtcga tgtcacgctg tgggtcgacg ctgccggatg ggtggggcac gctagcctcc
     3061 tgaaagcagc ggacaacgtg cacaaagccg tccatctggg tcaattggtt aagcaaagtc
     3121 cccgagatac ccgctttgct ggctccggcc tccaaaccgg caatatcggc ataggtgacc
     3181 ttggcataga cggtcttttg gggacggaag atggcggata gccgatcgac gcgttgatcg
     3241 ggaacatcca ccacggcggt gtgtacttca aaacgcccgc cgctcatggt ggtaggagca
     3301 ttgccgcgtg tc
//
LOCUS       DBNHOH010000900         2463 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_634859, whole genome shotgun sequence.
ACCESSION   DBNHOH010000900 DBNHOH010000000
VERSION     DBNHOH010000900.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_634859"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="AC16V7_16260"
     CDS             complement(<1..1110)
                     /locus_tag="AC16V7_16260"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR6258727.1"
                     /translation="MDASLRLGKIWEIPIGLHTSWFLVFGLLTWSLASGYFPEEYPNL
                     SSIVYIILALVTSLLFFGSVLAHELGHSFVAIRNRIPVKGITLFIFGGVAQISKEPGS
                     PGEEFRIAIAGPIVSLLLAGAFGVVWLFDQSIPILAAPSMYLLRINFMLAAFNMIPGF
                     PLDGGRVLRAIIWRINGNFQKATQTASYAGQLVAFGFIGWGVFTIFNGQFLNGLWLAF
                     IGWFLQNAAATAYAQTNMQSTLRSIHVSQVMERDCPRVPRLLTINQLVEDHILTGGQR
                     CFFVADDERLLGMLTLRDITQIPRPKWRFTTTEQAMIPLRNLVSVEPNTQLMSALQTM
                     DNAKINQLPVLIGEELVGMLSREQIVHYLRTRTELG"
     gene            complement(1247..>2463)
                     /locus_tag="AC16V7_16265"
     CDS             complement(1247..>2463)
                     /locus_tag="AC16V7_16265"
                     /inference="COORDINATES: protein motif:HMM:NF024241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6258728.1"
                     /translation="SPAPVGSTPVQRKNFINVQIDAIGIGLASALAPFLPVFLTRLGA
                     SSVQVGLLTSMPAFTGLLLAIPLGRFLQTRRQIVPWFGAARLMVVSSYAMTGLITFFV
                     PEKYLVNSILLIWAAATLPQTIVAVCFSVVMNAVAGPQHRYELMSRRWSILGVTTALT
                     VALVGQMLNRIEFPLNYQFAFLTLSIGGLISFYYSSHIRLPDAVLEETARGISWRDRF
                     RDYIRLITSEKAFVNFSLKRLFFMFGFTLAVPLYPLYYVREVHANDAWIGIISTVQSA
                     VLLVGYFLWTRGSRLRGSRFVLLRTTFGLAVYPALVALTTRVELIALFAGLAGIFQAG
                     LDLVFFDELMKTVPPQYSATFVSLAQSFQHLAAMLGPMLGTLLADHFGLGIGLFVSAI
                     LRLLGFILFSRR"
BASE COUNT          753 a          645 c          542 g          523 t
ORIGIN      
        1 ccctaattct gtacgtgtac gtaggtagtg gacaatctgc tcacgagaaa gcatcccaac
       61 aagctcctct ccaatcaaaa cgggcagctg attgattttc gcattatcca tcgtttgtaa
      121 agcgctcatc aactgcgtat tcggttcaac gctgactaaa ttccttagcg ggatcatcgc
      181 ctgttcagtg gtcgtgaatc gccacttagg gcgggggatc tgggtgatat ctcgcagagt
      241 caacatcccc aatagcctct cgtcatcagc aacaaagaaa cagcgttgac cacccgttaa
      301 tatgtgatcc tccactaatt ggttgatggt gagcaacctt ggcaccctag ggcaatctcg
      361 ttccatgacc tgcgagacat ggatggaacg aagtgtgctc tgcatgtttg tttgagcata
      421 ggcagtagcg gctgcattct gcaaaaacca cccgataaag gccagccaca atccattgag
      481 aaattgacca ttgaaaatgg tgaatactcc ccaaccaata aaaccaaatg cgaccagttg
      541 tcccgcgtac gaggcagttt gggtagcttt ttgaaagttt ccattaatac gccagataat
      601 tgctctcaga acacgtccac catccagggg aaatcctggg atcatattga aggcagccaa
      661 cataaaattg attcgcaata ggtacatgct cggcgcggcc aagatcggta tgctctggtc
      721 aaacagccag actactccaa acgcacccgc caataacagg ctgacgattg gcccggcgat
      781 ggcaatgcga aattcctcgc ccggggaacc aggctcctta cttatctgcg ccactccccc
      841 aaagatgaat agtgtaattc ctttgactgg tatccggtta cggatagcca cgaaggaatg
      901 accaagctcg tgagcaagca cagagccaaa aaacagaagg ctggtgacca acgctaaaat
      961 gatatagaca atgctcgaga gattcgggta ctcctcaggg aagtaaccgc ttgccagaga
     1021 ccatgtcagc aagccgaata ccaagaacca gcttgtatgc agccctattg gtatttccca
     1081 aattttaccc agtctcaaac tggcatccat tttatcctcc tagcctctta atataacatg
     1141 tcattagaga ctaacctaaa taaattatac ctattatgtc aagttattgt ccacattaag
     1201 tcaagtttct aataaaactc ttacactgtg aaccactcat aacccatcat ctacgcgaga
     1261 aaagtataaa ccctaatagc cgcaggattg cgctgacaaa caacccaatg cccagcccaa
     1321 aatgatcagc aagtaaggtt cccaacatag gtcccaacat agccgcaaga tgttgaaagc
     1381 tttgcgcaag cgacacgaac gtcgcactat attgtggtgg cacggtcttc attagctcgt
     1441 caaaaaaaac aaggtcgaga ccagcctgga aaattcccgc aagccctgcg aacagggcaa
     1501 tcaactccac ccgagtcgtg agcgcaacaa gtgctgggta gacggctaaa ccgaaagtag
     1561 tccttagcaa cacaaaacgg gaacctcgca gtcgcgaccc ccgtgtccat aggaaataac
     1621 caaccagcaa tacagccgac tggaccgtgc tgattatgcc tatccaggca tcattcgcat
     1681 gaacttcgcg cacataatac aagggataca atggcacagc aagcgtgaag ccgaacataa
     1741 aaaacaagcg cttcaatgaa aaatttacga aagccttctc gctggtaatg agccgtatgt
     1801 aatcacgaaa acggtctcgc caagatatcc cccgagcagt ttcctctaat actgcatctg
     1861 gcaagcgaat atgacttgaa taatagaagc taattaagcc cccaattgag agggttagaa
     1921 aggcgaattg gtaattcaag gggaattcaa tccgattcag catctgcccc actaacgcca
     1981 cggtcaaagc agttgtcact cccaaaatag accaacgcct actcatcagc tcatagcgat
     2041 gctgtgggcc agcaacagcg ttcatcacaa cagaaaaaca caccgccaca atggtttgtg
     2101 gcaacgtggc tgccgcccat atcagcaata ttgaatttac taagtatttc tctggcacaa
     2161 agaaggtaat caagccggtc atggcatagc tcgagactac catcaagcgg gctgcaccga
     2221 accagggcac tatttgacga cgggtttgca gaaaacgccc caagggaatt gctaggagca
     2281 aaccagtgaa tgcaggcatg gaggtcagaa gacccacctg aacactactg gcacccaggc
     2341 gggttagaaa cacaggtaag aacggtgcca gagcacttgc caagccaatg ccaatcgcat
     2401 cgatctggac attaataaaa ttcttacgtt gaaccggcgt ggagccaact ggagcaggcg
     2461 acc
//
LOCUS       DBNHOH010000901         3521 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_314470, whole genome shotgun sequence.
ACCESSION   DBNHOH010000901 DBNHOH010000000
VERSION     DBNHOH010000901.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_314470"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2102)
                     /locus_tag="AC16V7_16270"
     CDS             complement(<1..2102)
                     /locus_tag="AC16V7_16270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR6258729.1"
                     /translation="MPVKKGPRPVAGPSLVKSMRKTVLPRPVTPLAKPGTNPRLAEAR
                     VLRREAVRQVANKVQPNRPRPVVRPASAPVRVMPRTPAPPPATKAVRRPAVPASGVRV
                     AAATAAAAAGFLALNAAAAHTDIASEIRSLQSSLSNLERRSTFSDIESDVVNIDSSLQ
                     RASELLESARGKGYAFQGDLETTLYNTMSTWESMRPQVNNLVRQQKNALKSQLAGVNP
                     MIQSLNRVVGNPTAATAQLRSTQNHVNGLLHNISRIENDIQNRYDAVETQAQKLNTRL
                     TQIHWAMDQLVQAKFQLDSGEDLVMAVPTRWDKEGKDDPEGVLYLSNRRLLFEQKEKI
                     ATKKVLFITTASELVQELLIDQPHSGISGVKAESKGLFGHQDFLMVSLSDPRLGDVSL
                     HINGQDSKDWAALIERARSGQIEAERANVSASFSVGDLTKPLTPADIMALQSEVNALQ
                     DEVMLKSARQELAELENDVRSLERKLAEVRARGYAVEKDLEADITILAAQWERVKGNA
                     EGTIEYQARLLGDQMGAIQDDLAKVVGMSANLTAARPMYMQVKSALASLEAQADAAET
                     TVLAQYDEYADEVESLQAHLEWISWMLEALATASFRLLATESGVAATEAVHVHPSREP
                     ENGVLFLTDQRLLWEDRVGEYELKVDIPTQLVQDVRKETVDGAEMEALVVRISSGDLA
                     PETRFQLTLPVADDWIKMV"
     gene            complement(2121..>3521)
                     /locus_tag="AC16V7_16275"
     CDS             complement(2121..>3521)
                     /locus_tag="AC16V7_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIR6258730.1"
                     /translation="FKFDSEIFKGDVDYRFETLVQRFREMAFVTRGVTIFLMDERTDR
                     QMTFYFEGGITSFVRYLNRNRSVLHPVVHLEREVDGITVDAAIQYTDAFSESVYAFAN
                     TINTIDGGTHLTGLRAAITRTINDYARRNGLLKDSDPNFTGDDTREGLTAIVSIKHPD
                     PQFESQTKVKLMNPEVQTLSQQVVGEAFSVFLEENPSAGKSIVQKCLTSARARDAARK
                     ARDLVIRKSALESLTLPGKLADCSERDPQKTELYIVEGDSAGGSAKQGRDRHFQAILP
                     LRGKILNTERARLDKILANNEVKALISALGTSVGESFDVSGLRYGRVIIMTDADVDGS
                     HIRTLLLTFFFRYMLSLIEEGHLYIAQPPLYRIAYRNQIRYAYSDSEKDQVLESLGTS
                     SEKAVLSRYKGLGEMNPQQLWETTMDPAQRTLLQVTVEDAAEADRTFDMLMGSAVPPR
                     RRFIQTHARDVRNLDI"
BASE COUNT          791 a          995 c          879 g          856 t
ORIGIN      
        1 accattttta tccagtcatc tgccactggt agagtgagct gaaaacgagt ttccggagcc
       61 aagtcaccgc tactgatgcg cacaaccagg gcttccatct ctgcgccatc gaccgtctct
      121 ttgcgaacat cctggaccaa ttgcgttggg atatcgacct ttaactcata ttcgcccaca
      181 cgatcctccc acagaaggcg ctggtctgtc aggaaaagca cgccattctc tggctcacgg
      241 gatggatgga catgaactgc ctctgttgcc gctacaccgc tctcagtagc cagtaatcga
      301 aaagaagccg tagccagcgc ctccagcatc cagctaatcc attccaggtg agcctgaagg
      361 ctctctacct catcagcata ctcgtcatac tgtgccagaa cggtggtttc agcagcatca
      421 gcctgtgcct ctaaagaggc caaagccgac ttaacctgca tatacattgg gcgggctgca
      481 gtcaggttgg cagacatgcc gaccactttc gctagatcat cctggatggc acccatctgg
      541 tcgccaagca atcgagcctg atattcaatt gtgccctcag cgttgccctt gacccgctcc
      601 cactgtgcag cgaggatggt gatatcggcc tccaaatctt tctcgacagc atagccgcgg
      661 gcgcgcactt cggccagctt tcgctccagg gagcgcacgt cattctccaa ctccgccaat
      721 tcctgccttg cggatttcag catcacttca tcctgcaagg cattgacttc gctctgtagc
      781 gccataatat cggctggagt gagtggttta gtcagatctc ctaccgaaaa gctggccgat
      841 acgttagcgc gctcggcttc gatttgtccc gaacgggcgc gctcaataag cgcagcccaa
      901 tctttggagt cttgcccgtt tatatgcaac gagacatctc caagccttgg gtcactcaaa
      961 gaaaccatca gaaaatcctg gtgcccaaat aatcccttag attcagcctt cacgccactg
     1021 atgccgctat ggggttgatc aatgagcaat tcttgtacca gctcggacgc ggttgtaatg
     1081 aacaagacct tcttcgtggc gattttttcc ttctgctcaa acaaaagacg gcggttactc
     1141 aggtagagaa ccccttccgg gtcatccttg ccctctttgt cccagcgggt gggcaccgcc
     1201 atcaccaggt cttctccact gtcaagttga aatttggcct gaaccagctg atccattgcc
     1261 caatggattt gggtcaagcg cgtgttcagc ttttgtgcct gtgtttccac cgcatcataa
     1321 cggttctgga tatcgttttc tatccgtgag atattatgta ataggccatt gacgtggttt
     1381 tgagtgctgc gcagctgcgc cgtcgctgca gttgggttgc caaccacccg gttcaagctc
     1441 tggatcatcg gattgacacc agcgagctgg cttttcagag catttttctg ttgccgaaca
     1501 aggttgttga cctgagggcg catactttcc caagtactca tggtgttata caacgtggtc
     1561 tcaaggtcac cttgaaaggc atagccttta cctcgggcac tttctaacaa ctccgaagcg
     1621 cgctgtaagg atgaatcgat attgacaacg tctgattcga tatcactaaa ggtgctgcgt
     1681 ctctccaaat tggacaggga cgactgcagc gatcgaatct cagatgcaat gtctgtgtgc
     1741 gcggctgcgg cgtttaaagc caaaaaccct gctgctgcgg cagcagtcgc tgccgcgacc
     1801 cgtacaccgc tggcaggcac tgctggtctg cgcaccgcct ttgtggctgg agggggggct
     1861 ggagttctgg gcattactcg caccggtgca gaagccggac gcacaaccgg tcttgggcgg
     1921 ttaggctgaa ccttgttcgc aacctgccgg actgcctcgc ggcgcaacac cctggcttcc
     1981 gccaagcgag gatttgtccc cggcttagcc agtggtgtca cggggcgagg caatactgtt
     2041 ttacgcatag acttaactaa tgacggccca gccacagggc gcgggccttt tttaaccggc
     2101 ataattcctc ctatcgcact ttatatgtcc aggtttcgca cgtctcgggc atgggtttgg
     2161 atgaaacgac ggcgtggtgg gaccgctgaa cccatgagca tatcaaaagt gcgatcagct
     2221 tctgcagcgt cttccactgt aacttgcaag agcgttcgct gggccgggtc catggttgtc
     2281 tcccaaagct gttggggatt catctcgcct aaacctttat aacgagaaag aacggctttt
     2341 tctgagctag tacctaaaga ttcaagcacc tgatcttttt cgctatcgct ataggcgtaa
     2401 cggatctggt ttctataagc aatacggtac aacggtggtt gtgcaatgta caaatgaccc
     2461 tcttcgatga gcgatagcat atatcgaaaa aagaaggtta ataatagggt gcgaatatgg
     2521 ctaccatcaa cgtcagcatc ggtcatgatg atcacccgcc cataacgcaa acccgaaacg
     2581 tcaaagctct caccaacact agtccccagg gctgagataa gtgccttgac ctcgttattc
     2641 gccaggattt tatccaatcg cgcccgttcc gtattgagga tttttccccg cagcggcaaa
     2701 atcgcctgga agtgccggtc acgtccttgc ttcgctgacc cgccagccga atctccctcc
     2761 acgatataca gctcagtctt ctgtgggtcg cgctccgaac aatcagccag cttgccgggc
     2821 aatgtaaggc tctccagcgc agactttcgg attaccaggt cgcgcgcttt acgggctgca
     2881 tctcgcgccc gcgccgaggt caggcatttt tgcacgatcg atttgcccgc agacgggttt
     2941 tcttccaaga atacgctgaa agcctcaccc accacctgtt gcgagagggt ttgcacctcc
     3001 gggttcatca atttaacttt ggtctggctt tcgaactgcg ggtcagggtg cttgatgctg
     3061 acgatcgcag tcaaaccttc acgcgtgtcg tcgccggtga aattgggatc agaatcctta
     3121 agcaagccgt tccggcgggc atagtcattg atcgtgcggg taatggctgc acgtaaacct
     3181 gtcaggtgag tgccaccgtc aatggtattg atcgtattag caaacgcata tacagactca
     3241 gaaaaggcgt cggtgtactg aatggcagcg tcaacggtga taccatccac ctccctctcc
     3301 agatgaacca ccggatgcaa gacgctccga ttgcgattca gatatctcac aaaggatgtg
     3361 ataccgcctt cgaagtagaa ggtcatctgc cggtcagtgc gttcatccat caagaagatg
     3421 gtcacaccgc gcgtgacaaa agccatctct cggaagcgct gcaccagcgt ctcgaagcgg
     3481 taatccacat cgcctttgaa aatctcacta tcaaatttaa a
//
LOCUS       DBNHOH010000902         2685 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_57135, whole genome shotgun sequence.
ACCESSION   DBNHOH010000902 DBNHOH010000000
VERSION     DBNHOH010000902.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_57135"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="AC16V7_16280"
     CDS             <1..690
                     /locus_tag="AC16V7_16280"
                     /inference="COORDINATES: protein motif:HMM:NF024700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR6258731.1"
                     /translation="RLPRIKSLSPDMEENVRRLRAQLRRMGPINQEAQDEYHEVKQRY
                     EFMTEQVSDLQQAEADVQQVIVELDELMQRELRRTFDAVAEEFSEMFTRLFGGGSARL
                     LLTDPEDMSSTGVDIEARLPGKRMQGLSLLSGGERSLTATALIFALLKVSPTPFCLLD
                     EVDAMLDEANVGRFRELLRELSRNTQFVVVTHNRSTVQVADVIYGVTMGRDSTSQVIS
                     LKMDEVEKVVD"
     gene            complement(650..2113)
                     /locus_tag="AC16V7_16285"
     CDS             complement(650..2113)
                     /locus_tag="AC16V7_16285"
                     /inference="COORDINATES: protein motif:HMM:NF025065.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR6258732.1"
                     /translation="MKLRFDPAEQERFWDAIRRIPGYPVGDQIRLKTMLIESGALFRL
                     PSVLAALTEKSQGEVLVVMDPTPMRRGSDSLKPLVLDVLRNAGWQPNTLVLEPDSSGQ
                     VHTDMPHIEWVKARIQAGSPVVSVGSGVVTDIAKHACYLYEQETGIAIPYVVYQTANS
                     VSAYTSDMAPVFIEGVKRTLPSRYPDALISDLETLRDAPREMTVAGVGDLLAAFVSFP
                     DWYLAHQLGMDPKYTELPHALMDGMEELFLSNAEEIRLRSLPGMALLAKLIHLAGLAM
                     SLSHATTPLSGYEHVMSHILDLHNEQAGMPLAQHGSQVALTTILGSMAYEAFLDQFEP
                     AEVVVERCFPNAERIRLHILNEFAKIDPSGKAGEECWSDYRVKLERWSEQGSSLEGLL
                     ANWPQIKQRLQTLARPHETTLRILQAVEAPTNFEALIPPISESSAKFAFFNAPLMRYR
                     FTLGDMLLFLNWDREALWERIWKSFSPPPSPPRPFSS"
     gene            complement(2121..>2685)
                     /locus_tag="AC16V7_16290"
     CDS             complement(2121..>2685)
                     /locus_tag="AC16V7_16290"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HIR6258733.1"
                     /translation="TALYLKSASPGARTFVVGTTFLEEEFRQHGFTLDEENPEYAILG
                     FDTTLTYHKMWKLCDFVRAGLPYIATHPDLNCPTESGFMPDIGAMIAFVQASTGRLPD
                     KVIGKPNRAIIDELAKKIQLPVEHFAMIGDRLYTDIALGETTGITTILVLSGETQPSD
                     LETSPHKPDYIFNHLGEIAEYLTSNHH"
BASE COUNT          644 a          681 c          715 g          645 t
ORIGIN      
        1 cgtctgcctc gcatcaaaag cctctcgcca gacatggagg aaaatgtgcg ccgcctgcgc
       61 gcacagcttc gccgaatggg ccctatcaat caggaggctc aagatgaata tcatgaagtc
      121 aagcagcgct atgaatttat gacggagcaa gtcagcgatt tgcagcaggc tgaagcggat
      181 gtgcagcagg tcattgttga gctagacgag ctgatgcagc gcgagctgcg ccgtactttc
      241 gacgcagtcg ccgaagagtt ttccgaaatg tttacacgtc ttttcggggg tggttctgct
      301 cgcctgttgc ttacggaccc agaagatatg tccagcacgg gtgtggacat cgaggcgcgc
      361 ttaccaggta agcgcatgca aggactttct ctgctctcgg gtggggagcg tagcttgaca
      421 gcaaccgcat tgatttttgc cttgctcaaa gtatcgccaa caccattctg cctgctggat
      481 gaggttgacg cgatgctgga tgaggctaac gtgggacgct tccgtgaatt gctgcgagag
      541 ctcagccgaa atacacaatt cgtcgtcgtg acacataacc gcagcactgt tcaagtcgct
      601 gatgtgatat atggcgtcac gatggggcgc gattcaacca gccaggtgat cagcttgaaa
      661 atggacgagg tggagaaggt ggtggactaa aggatttcca tatcctttcc cacaacgctt
      721 ctcgatccca attcaaaaag agcagcatat cgcccagagt aaatcgatag cgcatgagcg
      781 gggcattaaa gaaggcaaac ttcgccgagc tttctgatat ggggggaatt aatgcctcga
      841 aatttgtcgg ggcttcaact gcctgaagga tgcgcagggt cgtctcgtga gggcgcgcca
      901 gagtttgaag cctctgcttg atttgcggcc aattagccag taagccttcc aaactgctgc
      961 cttgctcact ccacctttcg agtttgacgc gataatctga ccagcattct tccccagcct
     1021 tgccggatgg atcgatttta gcaaattcat tcaggatgtg aagccttata cgctccgcat
     1081 tgggaaaaca gcgctcaacg accacttctg caggttcaaa ttgatcaaga aaagcttcat
     1141 acgccatact gccaaggatg gtagtcagcg caacctggct accgtgctga gccaggggca
     1201 ttccggcctg ctcattgtgc aggtccagaa tgtgagacat cacatgctcg tatcctgaga
     1261 ggggcgtcgt cgcatgagag agcgacattg ccagcccggc gagatggatg agtttggcta
     1321 gcaacgccat accaggtaag gagcgcaggc gtatctcctc ggcgttactc aaaaagagct
     1381 cttccattcc atccatcaat gcatggggca attcagtata ttttggatcc attcccaatt
     1441 gatgggctaa gtaccagtcg gggaagctca caaaggcagc cagcaaatca cccacaccag
     1501 ccacagtcat ttcacgcggg gcatcgcgta aagtttccaa atcactgatt agcgcatccg
     1561 gatagcgaga tggcaaggtt cgcttcaccc cttcaatgaa taccggtgcc atgtcagacg
     1621 tgtaggcgct cacactgttg gctgtttggt agacgacgta tgggatggct attccagttt
     1681 cctgctcgta aaggtaacag gcatgcttgg caatatcggt caccacccct gacccaacag
     1741 ataccactgg agagccagcc tggatgcgtg cctttaccca ctcgatgtgg ggcatatcag
     1801 tgtgtacctg accgcttgaa tctggttcga gcaccagagt attgggttgc cagccggcgt
     1861 ttcgcaaaac atccagtacc aggggtttca ggctgtcaga tccacgcctc atcggggttg
     1921 gatccatcac caccaacact tctccctggg atttttccgt taacgcggct aaaacagatg
     1981 gcaatctgaa aagagcccct gattcaatca acattgtttt caggcggatt tggtcgccaa
     2041 ccggataacc tgggatacgc cggatggcgt cccagaatct ttcttgttct gctggatcga
     2101 atctcaattt catcgttttc ttaatggtga ttgctggtta gatattctgc aatttcgcca
     2161 aggtggttga aaatataatc tggcttgtgg ggagacgttt ctagatcaga tggttgagtc
     2221 tccccgctaa gcacaaggat ggtggtgatg ccagttgtct ccccgagagc gatatctgta
     2281 tacaggcgat ctccaatcat tgcaaagtgc tcaacgggta actgaatttt tttggcaagc
     2341 tcatcgatga tcgcccggtt tggcttgcct atgactttat ctggcaggcg gccagtggaa
     2401 gcttgtacaa atgcaatcat cgccccaata tcgggcataa aaccgctttc ggtggggcaa
     2461 ttgaggtcgg ggtgggtggc tatgtacggc aaacccgcgc gcacgaagtc acacagtttc
     2521 cacatcttat gataggtcag cgtggtgtcg aaacccagga tagcatactc aggattttcc
     2581 tcgtcgaggg taaaaccatg ctggcggaac tcttcttcca gaaatgtagt ccccacaacg
     2641 aaggttctcg cacccggcga tgcagacttt aaatacagcg cagtg
//
LOCUS       DBNHOH010000903         2826 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_955839, whole genome shotgun sequence.
ACCESSION   DBNHOH010000903 DBNHOH010000000
VERSION     DBNHOH010000903.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_955839"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..315)
                     /locus_tag="AC16V7_16295"
     CDS             complement(1..315)
                     /locus_tag="AC16V7_16295"
                     /inference="COORDINATES: protein motif:HMM:NF046853.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR6258734.1"
                     /translation="MSQTKSKIVKKRKRQNRSLVPLLLGLGGVVLVALAGIFIAMGGG
                     GKKASIVVQGAPSLKVDKEKLDFGDVKLGNWVEAEFTLTNVGDQPLRFTDQPYIEVVE
                     GC"
     gene            complement(312..1328)
                     /locus_tag="AC16V7_16300"
     CDS             complement(312..1328)
                     /locus_tag="AC16V7_16300"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR6258735.1"
                     /translation="MSREWLARILIISLVVAALAVPAWGWWQRSQGVLIHGRMAETGG
                     WSPENLTAEVGKPLKLRLTSDDVMHGFAVGQMAMQPVDMLPGEISEIVLNFERPGKYV
                     FYCSRWCSVNHWRMRGTIEVSGAAQAMPDDAEPPLYVQLGLDIDAEHHAEVLPERMPS
                     AAQGKALLASLPDNAIPTIYLGPDTYYTNSPQQLWQALRAEQGLSALSDQEVWDVVAA
                     IWESNTTPQALAEGQAMYSANCAACHGEGGAGDGVFAAQLTEHPSDHEEMQADQMSQP
                     PTDFTDPEHLLAASPAHLQGKIIRGGMGTGMPYWGPIFTEEQTWNLVAYLWKFQFNYE
                     ETEL"
     gene            complement(1332..2756)
                     /locus_tag="AC16V7_16305"
     CDS             complement(1332..2756)
                     /locus_tag="AC16V7_16305"
                     /inference="COORDINATES: protein motif:HMM:NF024211.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR6258736.1"
                     /translation="MNTLKELRWGKKAVEGRPQSTVQRLISQPGRTNLLRLPLMRFLL
                     ESRWPITLARGVTLAGFVFTLTAGLLGPRVGSHNFAIIFVWIAWWTALKLAFIPLGGR
                     SWCSVCPIPLPGEWLQQGGVLERGSRRLGLNLRWPKRLKGSWLQSGGFLLIGLFSAIT
                     LTDPRVTAWVLLALFGLAIALSLVFERRAFCSHICPIGGFTGLYAKTAPLEVRVVDTK
                     LCTTHSEKTCYQACPWGLYPVALRDAAACGLCMECLRACPRDNIAVNLRPFGSDLGAG
                     RSGIRLDDTFLALVMLGSALVFAAVFTGPWGALKSAAYAIGTSAWWGYVLGFLTLNLV
                     LLPGLFSLAVWSGHQASARKTPLKRLLADRSQVLLPLGLTAWIAFTVSFAFPKASYVL
                     AVLSDPFGWGWNLLGAASSIKLPDVTGLSQLLQTVLLLGGLFWSTRVAGRLASAEERS
                     LRQEVPLLAFCLVFTLVMFWLLIG"
BASE COUNT          636 a          887 c          770 g          533 t
ORIGIN      
        1 tcaacaccct tcaacgacct cgatgtacgg ttggtcggtg aaccgtaagg gttggtcacc
       61 cacattggtg agcgtaaatt ctgcttccac ccagttaccc agcttgacat caccaaagtc
      121 gagtttctcc ttgtccacct ttagactggg agcgccttgg acgacaattg aggctttctt
      181 gccgcctccg cccatggcga tgaatatacc cgccagcgcc accagcacca cgcctcccaa
      241 tcccaacaag agcggcacaa ggctgcggtt ttgtctttta cgttttttca cgattttgct
      301 tttggtttgg ctcatagttc ggtctcctca taattgaact ggaatttcca taaataagcg
      361 accaggttcc aggtctgctc ttcagtgaag attggtcccc agtagggcat accggtgccc
      421 attcccccac ggatgatctt gccttgcagg tgagctgggc tggcagccag tagatgctca
      481 gggtcggtaa aatcagtcgg cggctgggac atctggtctg cttgcatctc ttcatgatcg
      541 ctcgggtgtt cggtgagttg ggcggcaaac actccatcgc ctgcgcctcc ctccccatga
      601 caggcagcac agttggcaga atacatggct tgcccttccg ccagcgcttg cggggttgtg
      661 ttggactccc agatcgccgc caccacatcc caaacttcct ggtcagagag agcgctcaac
      721 ccctgttcag ccctgagcgc ttgccacagc tgttgcgggc tgttggtgta gtaggtatct
      781 ggccccaggt atatggtggg tatggcatta tcaggcaggc tggcgagcaa tgccttcccc
      841 tgggctgccg aaggcattcg ctctggcaag acttcagcat ggtgctccgc gtcaatgtcc
      901 agtcccagct gcacgtacag aggcggttcg gcgtcgtccg gcatcgcctg cgccgctccg
      961 cttacttcaa tcgtcccgcg catgcgccag tgattgacgc tgcaccagcg cgagcagtag
     1021 aaaacgtatt ttcccggtcg ctcaaaattc agcacgattt cgctgatctc accgggcaac
     1081 atatccaccg gttgcatcgc catctgcccc acggcgaagc cgtgcatcac gtcatcagat
     1141 gtgaggcgca gcttgagcgg cttgcccacc tcagcggtta ggttctctgg cgaccaaccg
     1201 ccggtttcgg ccatccgccc atggatgagc acgccttggc tgcgctgcca ccagccccag
     1261 gccggcaccg ccagtgctgc aacgaccagc gagataatca ggatacgggc aagccactct
     1321 cgactcatcg cttatccaat cagcagccag aacatgacca gcgtaaaaac caggcagaag
     1381 gccagcaagg gcacttcttg acgcaaggat cgctcttctg ccgaagccaa gcgaccggca
     1441 acccgcgtcg accagaacaa gccacctaat aacagaactg tttgcaacaa ctggctcaag
     1501 ccagtaacgt ccggcagctt gatagaactg gctgcaccca ataaattcca tccccagccg
     1561 aaggggtcag atagcactgc cagcacatag gaagccttgg ggaacgcgaa ggacacggta
     1621 aaggcgatcc aggcggtcaa gccgagtgga agcagcacct gggaacgatc agcgagcagg
     1681 cgcttcaagg gtgtcttgcg agccgaggct tgatgtccgc tccaaactgc caggctgaac
     1741 agtccaggca gcaacaccag gttgagtgtc aagaaaccca ggacgtaacc ccaccaggcg
     1801 ctggtgccga ttgcgtaggc agccgacttg agcgcgcccc acggtccggt aaacaccgct
     1861 gcaaagacca gcgctgaacc aagcattacc agggctagaa aagtatcgtc cagtcggatg
     1921 cccgatcgac ccgcacccag gtcgctgcca aacgggcgca ggttgacagc aatattgtcg
     1981 cgcgggcacg ctctaaggca ttccatgcac agaccgcagg cagcggcatc ccgcagcgca
     2041 actggataca agccccaggg gcaggcttgg tagcaggtct tctcgctgtg ggtggtacac
     2101 aacttcgtat ccacaacgcg cacttccagg ggcgcggtct tggcatacag tccagtgaaa
     2161 ccgccaattg ggcagatatg actacagaag gctctccgct caaagactaa ggagagagca
     2221 atcgccagcc cgaaaagcgc caggagcacc caggcggtca ctctcgggtc ggtgagcgtg
     2281 atggcgctga acagcccgat cagcaagaaa ccgccggact gcagccagga gcctttcaag
     2341 cgcttaggcc agcgcaggtt cagccctaac cgccggctgc ctcgctccag cacgcctccc
     2401 tgctgcagcc attctcccgg cagcgggatc gggcaaacac tgcaccagga acgcccaccc
     2461 aatgggatga aagccagttt gagcgctgtc caccaggcaa tccacacaaa gataatggca
     2521 aagttgtgac tgcctacccg gggaccgagc aggccagcag tcagggtgaa gacgaagcct
     2581 gctaaagtga ccccacgcgc cagggtgatc ggccagcggc tctccaatag aaagcgcatc
     2641 aagggcaggc gtaaaagatt tgtgcgccct ggttgagaga taagacgctg cacagtggac
     2701 tgtgggcgac cctctaccgc ttttttaccc cagcgaagct ctttgagcgt gttcatcttc
     2761 gccaccccca taccccggct accatcacca gagccgccag gctaaccacc cgccacatca
     2821 catagt
//
LOCUS       DBNHOH010000904         6654 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_506595, whole genome shotgun sequence.
ACCESSION   DBNHOH010000904 DBNHOH010000000
VERSION     DBNHOH010000904.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6654)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6654)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6654
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_506595"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /gene="nuoH"
                     /locus_tag="AC16V7_16310"
     CDS             complement(<1..1057)
                     /gene="nuoH"
                     /locus_tag="AC16V7_16310"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HIR6258737.1"
                     /translation="MDIALLLEWIVKSAILLLALVTGFAYLTFYERKALAKIQVRIGP
                     NRAGPGGWFQPLADGLKLIFKEELIPSKADRLMFVLAPVITVVPALIILGVVPWGDKV
                     TLFGREISLTIANINVGVLYLTAIASISVYGIVLAGWSSNNKYAMLGGIRSSAQMISY
                     ELTLGLSFVGPLLLAGTMDVAEIIEVQKELGWFIIWQPVGALVFWIATLAETNRAPFD
                     MPEAEQELTAGYHTEYSGMKFALFFMAEYIKMIAVSAIAAALYFGGYRGPFVDQIPWL
                     GPLYLFIKVILLLFGMIWVRATLPRIRYDRLMAFGWKILFPLALINVFITAVAIVLEV
                     LWLIPVLTIVSAAIVLYF"
     gene            complement(1135..3660)
                     /gene="nuoG"
                     /locus_tag="AC16V7_16315"
     CDS             complement(1135..3660)
                     /gene="nuoG"
                     /locus_tag="AC16V7_16315"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HIR6258738.1"
                     /translation="MSKLVTLTIDGLQVSVPAGTLLVNAAKKIGIDIPVFCYHPKMEP
                     VGMCRMCLVDIGRPVIDRATGQPVLEADGSPKIQFGPKLETACTTPVSEGMVVVGMSD
                     KVKAGREDILEFLLTSHPLDCPICDKGGECPLQNLTMDFGPGESRYLYDEKKHLAKRV
                     PLGELIFLDRERCIQCGRCVRFQDEIADDPVIGFYQRGRSLEIVTYSEPGFDSYFSGN
                     TSDICPVGALTTADFRFGARPWELNASASVCSQCPVGCNLTFNIRREAKAGGDWVIKR
                     VMPRQNEEVNEIWICDKGRFAYHFTESAERLTQPLVRKGGELVPTSWEEALKVVADHF
                     RQAGEGLLTLASGRLSNEDLFNLHELTTKLGGKTALYTDQAGGDLVAQVGVGQGTNFS
                     DMGPETAIFVVASDLLEEAPIWWLRVKQAAERGAQLIVANPRPTKLERYAKHTIRYPY
                     GSEAATVLAMVNALSAKRPNLPEAVQNLLRSPELQAAAKAFAEAENAVILFGGEGTGL
                     ETSQALSQACANLLITTGHVGKQNNGLIGVWQRANDQGAWDIGFRPLPGLKDAMRAAK
                     ALYVIAADPAGDDPAQAETGDFMVVQELFLTATAKLADVVLPVQAYTEREGTFTSGER
                     RVQRYYPAVPQRPGSKADFSVTAQIAKLLDQDLEARAASKVMQRIAASVPYYAGISYQ
                     KLAEVTEQWPIIGRQDLYYGGTGYENGQGLGVQLIPAAQRGETVPISWPQLSNVSVPE
                     NSLLAVPVTRLYDHGKTVMPSKLLHQRIPQTYVAMHPSEAARLKLDQSDMAEMSLNGS
                     STFVLLRLDEKLPTGVVLVPRSMGVPISGPIAAALRAVEKVTA"
     gene            complement(3727..5019)
                     /gene="nuoF"
                     /locus_tag="AC16V7_16320"
     CDS             complement(3727..5019)
                     /gene="nuoF"
                     /locus_tag="AC16V7_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HIR6258739.1"
                     /translation="MNKYLLLRNRELPTINRLEVYRQHGGFQAFHKVVTSMQPDEVIE
                     VVKASGLRGRGGAGFPTGLKWAFVDKKSWPHYVVANADESEPGTFKDREIMEGNPYQF
                     LEGVAIAAYAVQAKAAYVYLRGEFWQIASQLDQNIAALELAGLLGDNLFGTGYNLHIY
                     THLGAGAYICGEETALLESLEGKLGQPRLRPPFPPSYGLYGKPTVVNNVETLANVPLI
                     LDRGAEWYRTYGTEKSPGVKIFSLSGKVNRPGNYELPLGTTFRELIFEHGGGIPNRNK
                     IKAIMPAGASSSLIVATDQALDTPMDYESVPSVGAQLGSASIIVIDDSVNIPWLINKT
                     IHFFKHESCGKCTPCREGTYWMMHLTDRIADGKATQNDVELLHDVASQIKGKCLCALG
                     EFSIEAVLSGIDRFKDDFDLQTGGQQSVVQSEESAQTP"
     gene            complement(5016..5516)
                     /locus_tag="AC16V7_16325"
     CDS             complement(5016..5516)
                     /locus_tag="AC16V7_16325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013426.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE family
                     protein"
                     /protein_id="HIR6258740.1"
                     /translation="MLTEKYPEEIRTILAKYPSDQKRSAVMPLLYLAQREQGYVTKQS
                     MSEIAEILGISSTDVASIVGFYTLYYDEVGGKYHLQVCTDLPCALRGADEFLENLCQN
                     LGIRVGETTPDGLVTVEAVMCLAGCDKAPMFQVQSGDGLTYHENQTVESAMNLVKSWR
                     KEEGRA"
     gene            complement(5531..>6654)
                     /gene="nuoD"
                     /locus_tag="AC16V7_16330"
     CDS             complement(5531..>6654)
                     /gene="nuoD"
                     /locus_tag="AC16V7_16330"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HIR6258741.1"
                     /translation="THGVLRLLLELDGEIVVNCIPDVGFLHTGIEKNMEAKTYEKAAV
                     MTDRLDYLNTTGNNLVYCLAIEKLVDLDVPLRAQAIRVILTELQRIASHLVWLGTHAL
                     DLAAMSVFLYCFREREMILDVLEMCGGQRMMSTYFRPGGLWRDIPVEFEEAVRQILRV
                     FPARIDEYEALLTKNPLFIERTKKIGVLSRQDAIGWGITGPSLRASGVAHDLRKAQPY
                     SGYEQYDFVVPTRTDGDTYARYLVRIEEMRQSLLIVQQALDKLPYGPVRSNNRKFVPP
                     PRSELGVSMEALIHHFKLWTEGFNAPKGGVYIAVESPRGELGVYLEGDGGPKPHRVYW
                     RTPSFVNLQSLPLLSKGHLVADLVALIGTLDVVLGDVDR"
BASE COUNT         1541 a         1870 c         1813 g         1430 t
ORIGIN      
        1 aaaagtacaa cacaatcgcc gcgcttacaa tcgtcaacac aggaatcaac cataaaactt
       61 ctaacacaat tgccaccgcc gtgatgaaaa cgttgatcag cgccagcggg aagaggatct
      121 tccagccaaa agccatcaac cggtcatagc gaatacgggg caaagtagca cgcacccaga
      181 tcataccgaa aagtaagagg atgactttaa taaacagata caacgggccc aaccacggga
      241 tctggtctac aaagggtccg cgatagccgc caaagtacag ggccgcggcg atggcgctaa
      301 ctgcgatcat cttaatgtac tcagccatga agaacagggc gaacttcatg ccagaatatt
      361 cggtgtgata cccggcggta agctcttgtt ccgcctcggg catatcgaag ggggcgcggt
      421 tggtctccgc cagggttgcg atccaaaaga ccagcgcgcc caccggctgc cagatgatga
      481 accagcctaa ttccttctgg acttcaatga tctctgccac gtccatcgtg cctgccagca
      541 gcagcgggcc gacaaatgac aagcccaatg taagctcata gctgatcatc tgcgctgagg
      601 aacggatgcc gcccagcatg gcgtacttat tgtttgaaga ccagcccgcc aggacaatgc
      661 cataaaccga gatggaggca atagcggtga ggtacagcac gccaacgttg atattggcga
      721 tggtcaggga gatctccctg ccaaacaagg tgactttatc gccccaggga actacaccca
      781 agatgatcag cgccggcacc accgtaatca caggggccaa gacaaacatc agcctatctg
      841 ccttagatgg gatgagctct tctttaaata tcagcttgag cccatcggct aacggttgaa
      901 accagcctcc cggtcctgct cgatttggtc cgatgcgcac ctgaatcttc gccagggctt
      961 tgcgctcata aaaggtgagg taggcaaagc cggtcaccaa cgccaggagc aagatggccg
     1021 attttacaat ccattccagc aatagagcaa tgtccatagg cagtacctcg tgaaacgtat
     1081 aacgtaaagg ttatgtgtac ctaatttctg cgataccaga aacccagtca tgaatcaagc
     1141 cgtgactttt tctacagccc gcagcgctgc cgcgatgggc ccagagatgg gaacacccat
     1201 gcttcgcggc accagcacaa caccggttgg tagcttctca tctaggcgga gcaagacgaa
     1261 ggtcgaacta ccatttaagc tcatttctgc catatcgctc tggtcgagct tcaagcgagc
     1321 ggcttctgaa gggtgcatag ccacgtatgt ctgggggatg cgttggtgga gcaacttgga
     1381 cggcatcacc gtcttcccgt gatcgtacag gcgcgtcacc ggtaccgcca gcaagctatt
     1441 ctccggcacg ctgacattgg aaagctgcgg ccagcttata ggcacagtct cgccgcgctg
     1501 cgcggctggg atgagctgta cgcccaatcc ctggccgttt tcgtagccgg tgccaccata
     1561 atacaaatcc tgacgcccga taatgggcca ctgctctgtc acttcggcca gcttctgata
     1621 cgagataccc gcataatacg gcacgctagc cgcgatacgc tgcatcactt tggaagcggc
     1681 gcgagcttcc agatcctggt ccagcagctt ggcgatttgg gctgtcacgc tgaaatcggc
     1741 tttgctacca ggtcgctgtg gcacggccgg gtaatatcgc tgcacgcgcc gttcgccaga
     1801 tgtgaaagtg ccctcgcgct cggtgtaggc ttgcactggc aggacgacgt cagccagctt
     1861 ggcggtagca gtgagaaaca gctcctgcac caccataaaa tcacccgttt cagcctgggc
     1921 aggatcgtca ccagccgggt cagcggcgat cacatagagc gccttagctg ctcgcatggc
     1981 gtcttttaga ccaggcagcg ggcgaaatcc gatatcccaa gcgccctggt cattagcacg
     2041 ctgccagacg ccgatcaggc cattgttttg cttcccgacg tgtccggttg tgattaataa
     2101 attggcacag gcttgagaca gcgcctgtga ggtctctagc ccggtgcctt cgccgccaaa
     2161 caggatcacg gcgttctctg cttcggcaaa tgccttagcg gcggcctgca attcagggct
     2221 gcgcaagaga ttttgtacag cttctggaag gttgggacgt ttggcagata gggcattcac
     2281 catggctagc accgtagctg cttccgatcc ataggggtag cgaatggtgt gcttggcata
     2341 gcgctccagc ttggtgggac gcgggttggc tacaatcaat tgcgcgccgc gttcggcggc
     2401 ttgcttgacg cgcaaccacc agataggcgc ctcttccagc agatcactgg cgaccacaaa
     2461 gatggctgtt tccggtccca tatcggagaa atttgtcccc tgaccaacac ctacctgggc
     2521 aaccaggtcg ccacctgcct gatcggtgta tagagcggtc ttgccgccta attttgtggt
     2581 taactcatgc aggttgaaga ggtcttcatt cgaaagacgg ccgctagcca gggtcagcaa
     2641 cccctcacca gcttggcgga agtggtctgc aaccactttg agcgcttctt cccatgaagt
     2701 aggaaccagc tcgccgccct tgcgtacgag tggttgcgtc aagcgctctg cgctctccgt
     2761 gaagtggtag gcaaatcgtc ccttgtcgca aatccagatt tcattgacct cttcattctg
     2821 gcggggcatg acacgcttaa tcacccaatc cccaccggct ttggcctctc gacgtatgtt
     2881 gaaggtcagg ttgcaaccca ccgggcactg cgagcagacc gaggcggagg cgttcagctc
     2941 ccaggggcgt gccccaaagc ggaaatcggc ggtggtcaag gcacctactg ggcaaatatc
     3001 ggaggtattg ccggaaaaat aggagtcgaa tccgggttcg gaatatgtga cgatctccag
     3061 cgagcgacca cgctggtaaa aaccaatcac cggatcgtcc gcaatctcat cctggaagcg
     3121 gacgcaacgt ccgcattgga tacagcgctc acggtctaag aagattaact cgcccagcgg
     3181 cacgcgcttg gcaagatgct tcttctcgtc atatagatag cggctctcgc ctgggccgaa
     3241 atccatggtc aggttctgta gcgggcactc gccgccctta tcgcagatgg ggcaatcgag
     3301 agggtgggag gtgagcagga actcaaggat gtcctcgcgg ccggccttga ctttatcaga
     3361 cataccaaca acgaccatcc cttccgagac aggtgtcgtg caggcggtct ccagcttagg
     3421 accaaactgg atctttggcg agccatcggc ttctaaaacc ggttgtcctg tggcgcggtc
     3481 aatcactggg cggccgatat ccaccagaca catacggcac attcccacag gctccatctt
     3541 gggatggtaa caaaataccg ggatgtcaat gccgatcttt ttagcggcgt tcactagcag
     3601 tgtgccagca ggcacggaga cttgcaaccc atcaattgtc agtgttacca gtttgctcat
     3661 taaagatact ccaatgaaag cggatcgcaa tcggaggcca gcccatttgc cctcgatgtg
     3721 aagcaattat ggcgtttggg ctgattcctc actctggaca acggattgct gaccacctgt
     3781 ctgcaaatca aaatcatcct tgaagcggtc aatccccgaa agcaccgctt caattgaaaa
     3841 ctcacccagg gcacataagc atttcccctt gatctggctg gcgacatcgt ggagcaactc
     3901 tacgtcgttt tgggtggctt tgccatccgc gatacggtct gtgaggtgca tcatccaata
     3961 cgtgccctct cgacaagggg tgcacttgcc acaggattcg tgcttgaaga agtggattgt
     4021 cttattaata agccagggga tattaaccga atcatcgatg acgataattg aagctgagcc
     4081 aagttgcgcg ccaacgctgg gcacgctctc gtaatccatg ggcgtgtcca gagcctgatc
     4141 cgttgctacg atgagcgagg aagaggcgcc ggccggcatg atggccttga ttttgttgcg
     4201 gttagggatt ccgccgccgt gctcaaagat caactcgcgg aaagtagtac ccagcggcaa
     4261 ctcataattg ccaggacggt tgaccttgcc ggagagcgaa aagatcttca cgccgggact
     4321 tttctcagtg ccgtaagtgc ggtaccattc tgcgccgcgg tctaggatca atggcacatt
     4381 tgcaagcgtc tccacattgt tgaccactgt gggcttacca tacaacccgt aggagggtgg
     4441 aaagggagga cgaaggcgcg gttggccaag tttgccctcc agcgattcga gcaaggcggt
     4501 ctcttctccg catatatatg ctccagcacc caggtgggta tagatatgca ggttgtagcc
     4561 tgtaccgaaa agattatctc caagcaaacc agccagctct agcgcagcga tattttggtc
     4621 aagctggcta gcaatttgcc agaactcgcc gcgcaggtaa acataggccg ccttcgcctg
     4681 tacggcgtaa gcagcgatcg ctacaccctc caaaaactgg taggggttgc cttccataat
     4741 ctcgcgatct ttgaaggtac ccggttctga ttcgtcggca tttgcgacca cataatgtgg
     4801 ccaggatttt ttatcaacaa aagcccactt cagcccggtg gggaaccctg ccccgccccg
     4861 gccgcgaagc cctgaggctt tgaccacctc gatgacctcg tcaggctgca tcgaggtgac
     4921 gaccttatga aaggcctgga agccgccatg ctggcgataa acctccaacc gattgatggt
     4981 gggtagctca cggttgcgaa gaagcaggta tttattcatg cccgcccctc ctctttacgc
     5041 caggatttga ccaggttcat ggcgctttca accgtctgat tttcatgata ggtcaagcca
     5101 tcgccagact gcacctggaa catgggggct ttatcgcacc cagccaagca cataaccgct
     5161 tctactgtca ccagcccgtc gggtgtggtc tctcccacgc ggatgcccag gttttggcag
     5221 agattctcca gaaattcatc ggcgccacgc agggcgcagg gcaggtcggt gcaaacctgg
     5281 agatggtact tacctccgac ttcgtcataa taaagtgtgt aaaaaccgac aatagaagcc
     5341 acatcggttg aagatatgcc caatatttcg gcgatctcgc tcatcgattg tttggtcaca
     5401 tagccctgct cgcgctgtgc caggtagagc aacggcatta ctgctgagcg cttttgatcc
     5461 gaggggtatt tggctaggat agtgcggatt tcttctggat acttctcggt cagcatgtgc
     5521 cctcctctta tcaccggtcg acgtcaccga gtaccacgtc gagggtgccg attaaagcca
     5581 ccaggtcggc gaccagatgc ccttttgaca gcaagggcag agattgcaaa tttacaaaag
     5641 aaggtgtgcg ccagtagacg cgatggggtt tgggaccacc gtcgccctcc aggtacacac
     5701 ccagctcgcc gcgaggcgac tcaaccgcga tatagacgcc acctttaggg gcgttgaagc
     5761 cctcggtcca gagcttgaaa tggtggatga gcgcttccat gctgacgcct agctctgaac
     5821 gtggtggcgg gacgaacttt cggttgttgc tgcgcaccgg gccataggga agcttatcta
     5881 gcgcctgttg cacaatgagc agcgattggc gcatttcttc gatacgcacc aggtaccgcg
     5941 cgtaggtatc gccatcggtg cgggttggaa cgacaaaatc gtactgttca tatcctgaat
     6001 agggctgggc tttgcgcagg tcgtgcgcca ctcctgaggc gcgcaggctt gggccagtta
     6061 taccccagcc aatagcatcc tggcgggata gtacaccgat ctttttagtc cgctcaatga
     6121 acaggggatt cttggtgagg agcgcctcgt attcatcaat gcgagcgggg aaaacacgca
     6181 agatttggcg cacggcctcc tcaaactcaa cggggatatc ccgccatagg ccgcccggac
     6241 ggaagtaagt agacatcatg cgctgaccac cgcacatctc gaggacgtcc aggatcatct
     6301 cgcgctcgcg gaagcagtaa agaaaaaccg acatcgccgc caggtcaagg gcgtgcgtgc
     6361 ctaaccagac gagatgggaa gcaatgcgtt gcagctcggt aaggatgacc cggatagcct
     6421 gggcgcgcag gggaacatcc agatccacca gtttttcaat agccaagcag tagaccaagt
     6481 tattgcccgt tgtgttgaga taatccaagc ggtcggtcat taccgctgct ttttcatacg
     6541 tcttggcttc catgtttttc tcaatgccag tgtgcaggaa gccaacatct gggatgcagt
     6601 tgaccacgat ctcaccgtcc agctctaaca gcaagcgtag tactccgtgc gtgc
//
LOCUS       DBNHOH010000905         4862 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1789007, whole genome shotgun sequence.
ACCESSION   DBNHOH010000905 DBNHOH010000000
VERSION     DBNHOH010000905.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1789007"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2974)
                     /locus_tag="AC16V7_16335"
     CDS             complement(<1..2974)
                     /locus_tag="AC16V7_16335"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR6258742.1"
                     /translation="MTYQLFIIVVFALIPIFAIRWYKQPFIDLLTAFVIPYLVGLVYL
                     GTSIWIFNLRRSNTSSRLFSFLTASIGLNIAGFFDLFTTNYLATLWTANLMLIGGALI
                     NFALLFPEEARLLSRYPYLRWVGFVPSAIFVGYTLLTQKPQSYSNLLQLGYLYTWFAA
                     GVLVARITHQRLNSASPITREQSRLIIWGVVISFTPILVWLLSSIFKPGQEFSSIILV
                     PTVTFPVLTAYAMLRYRALNTDYLLSKTTLYATLSIVSAAGYALAVSGLSLVFGNLLP
                     PNHPLLVGLLVFLLAMLLNPLRLKLQRFVDSIFFRKQTDYQDRLQAFRRELTQTIELP
                     AIVQSLRTYIAKSLYPNQIHIFIRDEQSGQYAPEPGGDGKPTTDIRFPPNSVLVQILE
                     RRHGSIYLGGTETLPTLLQTERARLALLAVQLFVPLPGQSSLIGWLSLGTRQSGEPYS
                     NNDLRYLESLCDQAALAIERAQGVANLEQRVHEMDVLTRIAQGVNITLDFDDILELIY
                     AQTNVLIPTRDFRVTLRNQSNQALYYAFFLENDERLRDRENNNLLDGQTLEEVVIRSQ
                     RALLTDDYERESRGRGILAHEPDLFSWIGVPLNSGADTIGAISLGSRDPSKIFTQHHA
                     NLLQAIADQAAGAIIKARLLQESERRARQLATLNEVGKSLTSTLELRPLLNKILKSAT
                     DILNCEAGSLLMVDEHTSELVFEVTVGPVAAELIGQHLPPGTGKVGEAVQNRQPIIAN
                     DVRQSQGWFDQTDKKTGFITRDLLAVPMMLHERVTGVIELINKNDGSPFNQDDQELLA
                     TFASQAAIAVENARLYTQTDKALSARVEELSVMQRIDRELNVSLDLERAMRITLEWAM
                     RQSRTDAGLVGLLEGVIDDQATAIRVIAFQGYDAELSQSPDQDDNSPGARRLPVQLES
                     IYKAIESGQPYAVIQTENSSDETAADDPSQPPLILLSRGKSQVVVPIRRTSDVIGIIL
                     LESEHTNAYQEEIIAFLSR"
     gene            complement(3147..4361)
                     /locus_tag="AC16V7_16340"
     CDS             complement(3147..4361)
                     /locus_tag="AC16V7_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HIR6258743.1"
                     /translation="MSIAYPGHQNPEINFILREGHPDFSDLPWHLPLSKWAEICPRLE
                     DVPRGLTRHTVVFVNYDGILFALKEMPLRLAEKEYNTLIQIGALRIPAVTPIGLVSIQ
                     IPHDQIGVLITRYLEHSIPYRSLFMTGNLVRYREHLLDAISGLLVQLHLLGTYWGDCS
                     LSNTLFRRDAGALQAYLVDAETATIYPERTPASMRHQDLDIMEENVDGDIADLATAHL
                     LSAGIPTQDTGAYIRLRYQKLWEEITQEQIISSGESYRIQERIRALNDLGFSVSDMEM
                     LNTESGEQLRLRLVVSDRNFHRNQLFDLTGLEAEERQARQMMNEIQELRANLSHTHNR
                     STPLSVAAYQWLLEIYQPTIQRLNPLIKKDMNLAELYCQVLEHKWFLSEKAQQDVGHS
                     AAIDDYLIKSTE"
     gene            complement(4327..>4862)
                     /locus_tag="AC16V7_16345"
     CDS             complement(4327..>4862)
                     /locus_tag="AC16V7_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR6258744.1"
                     /translation="IVSQVPFLFSGSVLENICYASQDGVDFCDVEKIAMQIGEGEWLE
                     TLPQGLQTQVGERGVLLSMGQRQLISLMRVLVHKPVIFILDEATASIDPFTESQIQQA
                     LNMILANTTSIMIAHRLSTVIAADRIIVLDQGRIIEEGNHASLLAAKGQYASLYNTYF
                     RHQSLAYVDSLSWTSKP"
BASE COUNT         1220 a         1121 c         1293 g         1228 t
ORIGIN      
        1 gacgcgagag aaaggcgatg atttcttctt ggtaagcgtt ggtatgctca ctttctagga
       61 gtatgatgcc gatcacatcg ctggttctgc ggattggcac cacaacctgg ctttttcccc
      121 tagacaataa gatcaaaggc ggttgggatg ggtcgtcagc tgctgtttca tcactggagt
      181 tttcggtttg gataacggcg tacggttgac cggattctat ggccttataa atggattcca
      241 actggacagg taaacgtcga gcacctggag aattgtcatc ttgatctgga gattggctga
      301 gctcagcatc atatccttga aaggcaatca ctcggatagc cgttgcttga tcatcgatga
      361 ctccctcgag caaccctacc agaccagcat cagtgcgcga ttggcgcatt gcccattcca
      421 gggtaatgcg catggcgcgc tcgagatcta ggctgacgtt gagttcgcgg tcgatgcgct
      481 gcatgaccga gagctcttct acacgggcag agagagcttt atcggtctgg gtgtacaagc
      541 gggcattttc tacggcaatt gcagcctggc tagcgaaggt agctaacagt tcttggtcgt
      601 cctgattgaa aggtgaacca tcatttttat ttattaactc gatgacaccg gtaacccgct
      661 cgtgcaacat cattgggaca gccagtaaat cgcgcgtgat gaagccggtt tttttgtcag
      721 tttgatcaaa ccaaccttgt gattgtcgca catcgttggc gatgatgggt tgcctgttct
      781 gcaccgcctc acccactttt cctgttccag gtggcaagtg ttgtccgatg agctcagcgg
      841 cgaccgggcc aacagtaacc tcaaaaacaa gctcagaagt gtgctcatcg accatcagca
      901 ggctaccagc ctcgcaattc aagatgtcag tagcgctttt cagaattttg ttcagaagtg
      961 gtcgcagctc cagggtggaa gtaagacttt tgccaacctc gttgagggta gctagttgcc
     1021 gagctctccg ctcgctctcc tgaagtaggc gagctttgat gattgctcct gcagcttgat
     1081 cagctattgc ctggagcaaa ttggcatgat gctgggtaaa gatcttggat ggatcgcggc
     1141 ttcctaggct aatggcaccg atggtgtccg ctccggaatt caatggcaca ccaatccatg
     1201 agaaaagatc cggttcgtgt gccaggatgc cgcggccgcg ggattcgcgt tcataatcgt
     1261 ctgtcaacaa agcacgttga ctgcgtatca ctacttcttc gagggtttgt ccatctagaa
     1321 gattgttatt ttcgcggtct cgcaggcgtt catcgttttc tagaaaaaat gcataatata
     1381 aggcttggtt ggattgattt cgcaacgtca cccgaaaatc gcgggtgggg ataagaacgt
     1441 tggtctgagc gtaaatcaac tcgagtatat cgtcaaaatc tagggtgata ttcactccct
     1501 gggcaatgcg tgtgagcacg tccatctcat gtacgcgctg ttccaggttg gcaaccccct
     1561 gggcgcgctc aatcgccagg gctgcttgat cgcagaggga ttctagataa cgtaggtcgt
     1621 tgttagaata aggttcgcct gattgacgcg tccccaagct caaccagccg atcaggcttg
     1681 attgacctgg taagggcaca aaaagctgca cagccagcaa tgctaaacga gcgcgttccg
     1741 tttgcagcaa ggtgggcagc gtctcggtgc cccctaaata gatagagcca tgccgtcttt
     1801 caagaatttg caccaataca ctgttaggcg gaaatcggat atctgtagtg ggtttcccat
     1861 ctccacctgg ttctggagca tattgcccgc tctgctcatc cctgatgaaa atatgaattt
     1921 gattcgggta taagctttta gcgatatatg tgcgcaatga ctgcacgatg gcaggtaatt
     1981 caatggtttg agtgagttcc cgtctaaatg cttgtaggcg atcttggtag tcagtttgtt
     2041 tccgaaagaa tatcgaatcg acaaatcgtt gtaatttcaa gcgcagaggg ttgagcagca
     2101 tggcaagaag gaatactaac aagccaacca gcaaggggtg atttggggga agtaggttgc
     2161 cgaagaccaa gctgagcccg ctaactgcta atgcatatcc agctgcggag acgatagata
     2221 gcgtggcata tagggttgtc ttgctaagta aatagtcagt attgagggca cggtaacgca
     2281 gcattgcata tgctgtcagc accggaaatg tgactgtggg aaccaatatt atggatgaga
     2341 attcttgacc aggcttgaat atggaagaca ataaccaaac cagaataggg gtaaaagaaa
     2401 taaccacccc ccatatgatc aggcgggatt gctcgcgagt gatcggcgaa gcggagttta
     2461 atcgttggtg tgttatccgc gccaccaata cccctgcagc gaaccacgtg tacagatatc
     2521 caagctgcaa taaattggaa tatgattgcg gtttctgagt gagcagcgtg taacccacaa
     2581 aaatagcgct aggcacaaaa ccgacccagc gcaggtatgg atatcgcgag agcaatcttg
     2641 cttcttcggg gaaaaggagg gcaaagttaa tcagcgctcc gccgattaac attaggttcg
     2701 cagtccacaa tgtagctaaa taattggtgg taaataaatc gaaaaaccca gctatattta
     2761 gaccgattga ggctgttaga aatgaaaaca acctgctact ggtattgctg cgcctcaaat
     2821 tgaatatcca gatactggtg cccaaataga ccaacccgac gagatatggg atgacaaagg
     2881 cagtcagcaa atcgataaat ggttgtttat accaacggat cgcgaaaatc gggattaagg
     2941 caaaaacaac gatgatgaat agttggtagg tcaaaacagc cacagggcct gacctgtgtt
     3001 gcaagaaagc aagtaatgat gggcgcgcga accttagcgt tagacgcata gagtaatttt
     3061 atggcgtaat acgccagcgg tcaatttttg gaggccctgt ttagccttat ggttttgtaa
     3121 ggtatgccgc gcctttgctc attgaattat tctgtagatt tgataagata gtcatcaatg
     3181 gcagccgaat gacccacatc ttgttgggct ttctcagaca ggaaccattt atgctctaat
     3241 acctgacagt acaattcagc cagattcata tccttcttta tgagaggatt gaggcgctgg
     3301 atagttggct ggtatatctc tagcagccac tggtacgccg cgacgctcag gggtgtgctg
     3361 cggttgtgcg tatgcgagag gttggcgcgc aattcctgaa tttcgttcat catctgccgg
     3421 gcttgccttt cctctgcctc caatccggtc agatcgaaaa gttgattgcg gtggaagtta
     3481 cggtctgaga ctaccaagcg caggcggagt tgttcgccgc tttctgtatt tagcatttcc
     3541 atatcactta ccgagaagcc cagatcgttc aaggcgcgta tgcgttcctg tatgcgatag
     3601 ctctctcctg aggagataat ctgctcctga gtgatctctt cccacaactt ttggtaacgc
     3661 aagcggatgt aagcgcctgt atcttgtgtt ggtataccag cggagagtag atgggcagta
     3721 gccaggtctg caatatcacc atctacgttt tcttccataa tatccagatc ttggtggcgc
     3781 atgctcgctg gggtgcgttc gggatatatc gttgcagtct ctgcatctac caggtaagct
     3841 tgcagtgcgc ctgcatcgcg gcggaagagc gtattggaaa gcgaacaatc gccccaatag
     3901 gttccgagta gatgaagttg cacaagcaac ccagagatgg cgtcaagtag gtgttcgcga
     3961 taacgcacaa gatttccggt cataaaaagc gaccgatacg ggatagaatg ttccaggtag
     4021 cgtgtaatca agaccccaat ctggtcgtgc gggatttgga tgctcactaa tccaatcggt
     4081 gtaacagcgg gtatgcgcag cgcaccgatt tggatcagag tgttatattc tttctcagcc
     4141 aggcggagcg gcatctcttt caaggcgaat aggatgccat cgtagttcac aaagaccacc
     4201 gtgtggcgag tcagaccgcg cggaacatcc tctaagcgcg ggcaaatttc agcccacttt
     4261 gaaaggggca ggtgccaggg caggtcgctg aaatcggggt gaccctcacg caggatgaaa
     4321 ttaatctcag ggttttgatg tccaggataa gctatcgaca taggcaaggc tctgatggcg
     4381 gaaatatgta ttgtacaggc tagcgtattg tcctttagcg gctagcaaac tggcatgatt
     4441 accttcttcg atgatgcgcc cctgatccag gacaataatg cgatctgcag cgataactgt
     4501 agataagcgg tgagctatca ttatgctggt ggtgttagcc aggatcatat ttagcgcctg
     4561 ttggatttgg gactcggtga atggatcgat gctggcggtg gcctcatcta atataaatat
     4621 cacgggttta tgcactagga cacgcatcag ggagatcaac tggcgctgcc ccatggatag
     4681 caacacgccg cgctcaccga cttgggtttg cagaccttga ggcagcgtct caagccactc
     4741 cccttcgcca atttgcatgg cgattttctc gacgtcacag aaatccaccc cgtcctgcga
     4801 tgcatagcag atattttcca gcacgctgcc tgagaagagg aagggtacct gggagacaat
     4861 tc
//
LOCUS       DBNHOH010000906         1036 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1724546, whole genome shotgun sequence.
ACCESSION   DBNHOH010000906 DBNHOH010000000
VERSION     DBNHOH010000906.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1724546"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..701
                     /locus_tag="AC16V7_16350"
     CDS             192..701
                     /locus_tag="AC16V7_16350"
                     /inference="COORDINATES: protein motif:HMM:NF019533.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HIR6258745.1"
                     /translation="MDSLQAFRAEKDQFFAHHPQSPLTREQKANFQGLDYFPETPDLR
                     LEVDVEEFAEKNHIEMQTTTGDVQSYIRFGKIRFQVDGQDAELTVYANQHGFFLPFVD
                     SLAGKETYPAGRYLEPELQHNGKLLIDFNMAYNPYCAYNEMWSCPLTPFENRLKVPIR
                     AGERVFQSQ"
BASE COUNT          269 a          225 c          263 g          279 t
ORIGIN      
        1 gtctgctcag gcatccaaca atgtctccta tactagtaat ttcaatcaca agtgtagaat
       61 ataacctatg agatgtatac tgagatttgc acaggctaat attagtagtt gataaagata
      121 tctggccaag taagacgcac atttaataca gcgtctttaa cctaaataag tattctgttt
      181 ggaggatttg catggattct ttgcaagctt ttcgtgctga gaaggatcaa tttttcgccc
      241 atcaccccca aagcccgcta acccgcgagc aaaaggctaa ctttcaaggc ttggattatt
      301 ttccggaaac tcctgatctg cgcttggaag tggatgtgga ggaattcgcc gagaaaaacc
      361 acatcgagat gcagaccact actggagatg ttcaatcgta cattcgcttt ggcaaaatac
      421 gtttccaggt ggatgggcaa gacgccgagc tgactgttta cgccaaccag cacggtttct
      481 ttttgccgtt cgtggacagc ttggcgggta aggagactta cccggctggg cgctatctgg
      541 aacccgagct acagcacaat ggcaaattgt taattgattt caacatggcg tacaacccgt
      601 attgcgctta taacgagatg tggtcatgcc ctctgacccc attcgaaaac cggctgaagg
      661 tgcctatccg cgctggcgag cgggtgtttc agtctcagtg attgtgcgat cgatgattcg
      721 cctgtgcccc ggggttactt acctggtgat accaaaatgc ctgacaacag cgatcttatt
      781 gaggaaatct tggtgacgat aagggataga ttctttggtt ggatcgctgg cggtcctcat
      841 aggcgggtgt ttaatccagc cagatgacga gaggcgcgcc tggctggaga ggaattctgg
      901 ggaaatccgc ctgagctaca cggcatatga ttggggattg aatatcgagc gcgattcaga
      961 atagggggtt ttggagcatt ttagaagcca aaatgcttga ccttcgatat attgtttgat
     1021 attatataaa tcaccc
//
LOCUS       DBNHOH010000907         4386 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1660330, whole genome shotgun sequence.
ACCESSION   DBNHOH010000907 DBNHOH010000000
VERSION     DBNHOH010000907.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1660330"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2121
                     /locus_tag="AC16V7_16355"
     CDS             <1..2121
                     /locus_tag="AC16V7_16355"
                     /inference="COORDINATES: protein motif:HMM:NF013720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcvT family protein"
                     /protein_id="HIR6258746.1"
                     /translation="NFARGYGINVEEISPKEFKELWPLADARDVLAGFYTPEDGRANP
                     VDATLSLAKGARNQGARIYEETEVTGIKKKNGRVTGVVTNRGEIEAEFVVNCCGMWAR
                     KVGQLAGVDVPLHAAEHYYLITEPMEGVSEDLPILEDVDRYAYYREEVGGLMLGLFEP
                     VAAPWGMGGIPRDFAFGEIQPDWDRMMPYIDKAMERIPAIKAAGIRKLFCGPESFTPD
                     LTLLLGEAPELRNFYVAAGLNSLGILLGGGVGKVLAQWIIDGLPPVDVADIHIDRMLP
                     FQNTPQYLHDRTVEILGMMYKESFPNSQFVTARNARKSILHERLAKAGAYFASSAGWE
                     YPDWFAPPGVEPRVEYSWGRQNWFEYNAAEHKACRQDVILMDLSLMSKFLVQGRDAEA
                     ILNRISTNDVSIPVGKIVYTEWLNERGTIEADLTVTRVAEDAFLVICSDLAHRHVETW
                     LKRHIPPEAHAFVTDVTSGYAMINVQGPKSRQLLSRVTNTDLSNEAFPYMTMKEIDIH
                     YARAMAYRITYLGELGYELYIPTEFAPSIFDLLVEAGQDLGLKHAGLQALNTLRIEKA
                     YRDYGHDIDVTDTPLEAGLSFVVDFNKPSGFIGREALLRQKESGILKNRFAQFLLEDP
                     GPLLYYGEQIYRDGKWVGYVRSGAYGFTLGGAVGLAFIENEEGVTADYIRSGKFEIEV
                     NKVRYPAKVSLRPMYDPKNERVKC"
     gene            2167..3618
                     /locus_tag="AC16V7_16360"
     CDS             2167..3618
                     /locus_tag="AC16V7_16360"
                     /inference="COORDINATES: protein motif:HMM:NF018012.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR6258747.1"
                     /translation="MTPDRLPIEPIVSKHRIQFFSDEDLDQLQEATLQVLEKTGVQFP
                     TEKALSIFEEHGALVDRDTSVVKLPRDLVIKAMSTVPRYYRLGARDPALGFSLQEGYT
                     YMGVDGCGVETVDPKTGLTRSSTKADVGRTAKIVDYLSSTAFYWPMVGSQDFGKLSSL
                     HDLDASWNNTVKHIKTETLLGEVATRYAIEMATVVAGSRQALRENPLLSVLICTIAPL
                     VQDKEGIEGAMLLAEEGIPVSFMAMPTMGTTAPVTQAGAFVVADAEIISATVLMQLIN
                     PGTPVSHSLLQGWVDPRNGNYIAYPVDARVRYGPIDIAHHWGMPSFGGAFGTESVEPG
                     TWQSAADVSLDPLLISLASCEWATGIGLNRTFTLLHPEAIILDDEIYHRARYALAEEE
                     VSPETLALDVIDKVGPGGHYLSQKHTRKYIRTTVRLGLTHVTDQTGKYRHPLEVAREK
                     FDWILQNHQPPPLEADKQAELNKILASAEKEIV"
     gene            3654..>4386
                     /locus_tag="AC16V7_16365"
     CDS             3654..>4386
                     /locus_tag="AC16V7_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR6258748.1"
                     /translation="MDGQKTPINRDRLRALIAQEQQRFVAEHPASKKLFEKAKAHLLD
                     GVPMNWMVEWAGTFPIFVKEGSGAYLWDVDGHRYLDLCLGDTGAMTGHSPHAAVQAVT
                     DRLGRGITFMLPTEDAVWLGEELHRRFGLPYWQIALTATDANRFTIRLARYITGRKRI
                     LVFNGCYHGTVDESFVTIVDGKPVSREANAGPPVDPTLTTRVIEFNDIPALEAALQPG
                     DVACVLAEPALTNIGIVLPEPGYHAA"
BASE COUNT         1073 a         1140 c         1181 g          992 t
ORIGIN      
        1 aatttcgctc ggggttatgg catcaacgta gaggagattt ccccaaagga gttcaaggaa
       61 ttgtggcctc ttgcagacgc acgcgatgtg cttgccgggt tctacacgcc cgaagacggt
      121 cgtgcaaacc cggtagacgc aactttatct ctagccaaag gggccaggaa tcagggtgcg
      181 cgtatctacg aagaaacaga ggtcaccggg ataaagaaaa aaaatgggcg ggtgactggt
      241 gtcgtgacga accgcggtga aattgaagcc gaatttgtgg tgaactgctg cgggatgtgg
      301 gccagaaaag tcggtcaact ggctggggta gatgtacccc tgcatgctgc cgagcattat
      361 tacttgatca ccgaaccgat ggaaggggtc tctgaagatc tgcccatatt agaagatgtt
      421 gaccgttatg catactaccg ggaggaagtg ggcggcttga tgctagggct gtttgagcca
      481 gtggctgcgc cctggggcat gggtggaatc ccgcgagatt tcgcctttgg cgagattcaa
      541 cctgactggg atcgaatgat gccttacatt gataaagcca tggagcgcat tccagcgata
      601 aaagctgccg ggatcagaaa actattctgc ggtccagaaa gtttcacccc cgatttaacc
      661 ctcttgctag gtgaagcgcc agaactgaga aatttttacg tggcagctgg tctaaactcc
      721 ttggggattt tgctcggagg cggcgtaggt aaggtcctgg cgcaatggat catagacggt
      781 ttgccgccgg tagatgtcgc tgatatccac attgacagga tgttgccctt ccagaatacc
      841 ccccaatacc tgcacgatcg caccgtggaa atcctaggca tgatgtacaa ggagagtttt
      901 cccaactccc agtttgtgac tgccaggaat gcgcgcaaat ccatcctgca tgagcgtttg
      961 gcgaaagcag gcgcatattt tgcctcctcc gccgggtggg aatacccgga ctggtttgcg
     1021 cctccaggtg ttgagccacg ggttgaatac tcttggggac gccagaattg gtttgagtac
     1081 aacgccgccg aacacaaagc ttgccggcaa gatgtgatct tgatggatct ctcactgatg
     1141 tctaaattcc tcgttcaagg gagagatgca gaagcaatcc tcaaccggat ttcaaccaat
     1201 gatgtctcca tcccggtagg taagatcgtt tacacggagt ggctaaatga acgcggaaca
     1261 attgaagccg atcttaccgt gacacgggtg gcagaagacg ccttccttgt gatctgctcg
     1321 gacctggccc atcgccatgt agaaacctgg cttaagcggc atatccctcc agaggcgcat
     1381 gccttcgtca ccgatgtcac ttcgggctac gccatgatta atgtccaggg accaaaatca
     1441 cgccaactcc tgagccgtgt gacaaacaca gacctatcca atgaagcctt cccctatatg
     1501 accatgaagg aaatagatat ccattatgcg cgcgcaatgg cttatcgaat tacttatctt
     1561 ggcgagcttg gatacgaatt gtatatcccc accgaatttg ccccctcgat ctttgacctg
     1621 ctagttgagg ccggacaaga cctgggatta aagcacgccg gtcttcaagc gctgaacacg
     1681 ctcaggatcg aaaaagccta ccgggattat ggtcatgata tcgatgtgac cgacacccca
     1741 ctggaagcag gcctgagctt tgtggtcgat ttcaacaaac ccagtggttt tattgggcgc
     1801 gaggcgctgc tcagacaaaa ggaatctgga attctaaaaa atcggtttgc ccaattcctt
     1861 ctagaggatc ccgggcctct cctttactac ggagaacaaa tataccgcga tggcaaatgg
     1921 gtcgggtatg tccgttcagg cgcctatgga ttcacgcttg gtggagctgt aggattagct
     1981 ttcatagaga atgaagaagg tgtaaccgcc gattatatcc gtagcgggaa gtttgaaatt
     2041 gaggtgaaca aggtgcgcta ccccgcgaaa gtctcactgc ggccgatgta cgatcccaag
     2101 aacgaacggg tgaagtgctg aacagctgac ttaaaaataa ccctatcgat ttccaggaga
     2161 tatattatga cccctgatcg cttaccgatt gagccaattg tatccaagca caggatccaa
     2221 ttcttctccg atgaggatct ggaccaattg caagaagcaa ccctccaggt gcttgaaaaa
     2281 acaggtgtgc aattcccgac agaaaaagcc ctgtccattt ttgaggaaca cggcgctctg
     2341 gtggacaggg atacaagcgt tgtcaagcta ccccgggacc tggtcatcaa agccatgtcc
     2401 actgttccgc gctattaccg attgggagcc cgcgatcctg cgctgggttt ttctctccag
     2461 gaaggatata cctatatggg tgtggatgga tgcggagtgg agaccgttga tcccaaaaca
     2521 ggtctgaccc gctcctctac gaaagcagac gtgggcagaa cggcgaagat cgtcgattat
     2581 ttatcctcca ccgcgtttta ctggccaatg gtcggctcgc aagatttcgg taaattatcc
     2641 tctttgcacg atttggacgc atcctggaac aatacagtca agcacattaa aaccgagaca
     2701 ctgctgggag aggtcgccac tcgctatgcc atcgagatgg ctacagttgt cgcagggagc
     2761 agacaggcgc ttcgggaaaa cccgttgtta tccgtgctaa tatgcaccat tgcgcctttg
     2821 gtgcaagata aagaagggat tgagggcgcc atgctcctgg ctgaggaagg cattcccgtg
     2881 agtttcatgg ctatgcctac tatgggcacg actgcacccg ttacacaagc cggtgcgttt
     2941 gtggttgcag atgctgagat catcagcgcg acggtgctca tgcagctgat caatccgggc
     3001 acaccagtta gccactcctt gttacaaggc tgggttgacc cgcgaaatgg caactacatc
     3061 gcctatccag tcgatgctcg cgttcgttac gggcccatag acatagctca tcattggggt
     3121 atgccttcgt tcgggggcgc ttttggaact gagagcgttg aaccagggac ctggcaatcg
     3181 gccgccgatg tgtctctgga cccattgttg atcagtttgg catcttgtga gtgggcaacc
     3241 ggtattggtc taaaccgcac cttcacccta ctccatccag aggcaatcat cctggatgat
     3301 gagatctatc atcgagctcg ctacgccctg gcagaggagg aggtatcacc tgaaacactt
     3361 gcactggatg tgatcgacaa ggtcggtccc ggcgggcatt atttgtctca aaaacacact
     3421 cgcaagtata tccgcactac cgtaagactc ggtcttaccc atgtcaccga tcaaaccggg
     3481 aaatatcgcc atccactcga agtcgctcgt gaaaagttcg actggatctt gcagaaccac
     3541 caaccccctc cccttgaagc tgacaagcaa gccgagctga acaaaatcct cgcctctgca
     3601 gaaaaagaga tcgtctaggt ggcaactcaa aaccaaattg gggattgaaa ggcatggatg
     3661 ggcaaaaaac accaatcaat agggacagat taagagccct cattgcccag gaacaacagc
     3721 gcttcgtggc agaacatcct gcctcgaaga agcttttcga gaaggcaaaa gctcatttgc
     3781 tggatggcgt gcccatgaat tggatggtcg agtgggctgg gacgtttccc attttcgtca
     3841 aggaaggatc aggggcttat ctatgggatg tcgatggcca tcgttatctc gacctgtgcc
     3901 tgggcgacac aggtgctatg acaggccatt cgccgcatgc tgctgtccag gccgtcaccg
     3961 atcgcctggg ccgcggaatc accttcatgc tgcctaccga ggatgccgtc tggttgggag
     4021 aagagctgca ccgtcgcttc ggtttgccat actggcaaat cgccttgacc gccacagacg
     4081 ccaatcgctt cacaatccgc ttagcgcgtt acatcaccgg acgcaaacgc atattggtgt
     4141 tcaatggttg ctatcacggc acggtggacg agtcgttcgt caccatcgtg gatgggaagc
     4201 ccgtatcgcg cgaagccaat gcaggcccgc cagtggatcc caccctgacc acgcgggtga
     4261 tcgagttcaa tgatatcccc gccctcgaag ccgccctgca gcccggcgat gtggcctgcg
     4321 tgctggccga acctgcactg accaatatcg gaattgtgct tcccgaacca ggttatcacg
     4381 cggctt
//
LOCUS       DBNHOH010000908         2817 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_121827, whole genome shotgun sequence.
ACCESSION   DBNHOH010000908 DBNHOH010000000
VERSION     DBNHOH010000908.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_121827"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(263..706)
                     /locus_tag="AC16V7_16370"
     CDS             complement(263..706)
                     /locus_tag="AC16V7_16370"
                     /inference="COORDINATES: protein motif:HMM:NF016001.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HIR6258749.1"
                     /translation="MSQTPTIPPFVNRTMKLVLRSPVHGIVSKTVLLITFTGRKSGKT
                     YTTPVSYSQNGDQVYIFTHADWWKNLRSGAPVSLRIQGRELQGLAEAVAEDKSAVATG
                     LAAHLRKVPSDARYYGVTFDDHRNPRAEEIEKAAQSVVMIRIRLC"
     gene            complement(720..899)
                     /locus_tag="AC16V7_16375"
     CDS             complement(720..899)
                     /locus_tag="AC16V7_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005986542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258750.1"
                     /translation="MKTDRSTLTQKTFACETCTMRKKAEANPKTLMARLWRWHTGWCP
                     GWKAYQAHLAEQMKP"
     gene            complement(934..2187)
                     /locus_tag="AC16V7_16380"
     CDS             complement(934..2187)
                     /locus_tag="AC16V7_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006512747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HIR6258751.1"
                     /translation="MIIIAYVLSLLPLVLNASMFIHLKPPYNYYFWAFQIAAVVLSPV
                     LVVLSLLGAGLGWLYHTPIPVAAGVLSAGVSILYIARVTVPQPDFDLAFGKDWKARIP
                     SSRASHMLKRRWNLGWPRTGEPRWERNIPFWTIPGTDRKLLCDVWQPPDGVPYSGLAL
                     VYLHASAWWILDKDSGTRPLFRHLAAQGHVIMDVAYRLCPEVDIYGMIGDVKRAVAWM
                     KANADRYQVDPERIVLGGASAGGHLALLAAYTPDHPSLNPPDVQESDLSVRAVVSYYG
                     PTDLRACYHHYNQERVIGMPKVEVGLPGAADMKKDMTQAGRMDTLLGGHLHEVPEIYE
                     LASPVAHVDTECPPTLLIQGEPDAFVPVPATRALHRKLTKCGVPSVMIIYPLANHAFD
                     LILPKISPPAHAAHYYLERFLALIT"
     gene            complement(2184..2426)
                     /locus_tag="AC16V7_16385"
     CDS             complement(2184..2426)
                     /locus_tag="AC16V7_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258752.1"
                     /translation="MPEYYEIKIKGYLNEHWSDWFAGLKLTHLELEETLLSGTLPDQA
                     ALHGLLERIRDLNLTLISVTCGCPPAQDSDEDVKPS"
     gene            complement(2654..2809)
                     /locus_tag="AC16V7_16390"
     CDS             complement(2654..2809)
                     /locus_tag="AC16V7_16390"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6258753.1"
                     /translation="MALGKSNREIAGQLFVSPGTVKAHTSSIYRKLDVANRTEAVARA
                     RQLGILP"
BASE COUNT          583 a          734 c          839 g          661 t
ORIGIN      
        1 cggtcgatgc ttcttcaggc atggggagct ggatatcgac agtctgggtg gtccctgttt
       61 cgatttgagt aacaggcggt ttgatgttga tgccgcaggc catggtaacc agggccagaa
      121 cgagaacggc ggcaagaata tgtttgcggt ggttcatttt tttctcctga tgagtacctg
      181 tttatagtgt agcgtgtgct gaaaaagctc gcatctgcca aagggtatat ctgaggttta
      241 tctaaagggt ttactttagg gatcagcata ggcggatgcg gatcatcacc acagattgtg
      301 cggctttctc aatttcttct gctcttgggt ttctgtggtc gtcgaaggtc acaccgtaat
      361 acctggcgtc gctcggcacc ttccgcagat gcgctgccag cccggtggcg acagcactct
      421 tatcctccgc gacagcttct gccaggccct ggagctctcg tccctggatg cgcaatgaga
      481 ccggggcacc gctgcgcagg tttttccacc agtctgcatg ggtaaaaatg tatacctgat
      541 ctccgttttg agaatagctg actggggtgg tgtaggtctt cccgctcttg cgcccggtga
      601 aggtgatcag caggacggtt ttgctgacta ttccgtgcac aggcgagcgc aagacgagct
      661 tcatggtgcg attgacgaag ggggggatag tgggagtttg ggacatttca gcctctttct
      721 tatggtttca tctgctcggc taggtgtgcc tggtaagctt tccagccggg gcaccagccg
      781 gtgtgccagc gccacagtcg tgccatcagg gttttggggt tggcttcagc ctttttcctc
      841 atggtacagg tttcgcaggc gaatgtcttt tgagttaagg tcgagcgatc ggttttcata
      901 gctttattct ttcatggtgg aatttcgaaa tcgtcaagtt atcagcgcca ggaagcgctc
      961 caggtagtag tgtgccgcat gggctggagg ggaaatcttc ggcaatatca agtcgaaggc
     1021 gtggttcgcc aggggataaa tgatcatgac cgacggaact ccacatttgg tcaacttgcg
     1081 gtgcagcgcg cgcgtggcgg gcacgggcac aaacgcgtcc ggctcaccct ggatgagcag
     1141 cgtgggcggg cactcagtgt ctacatgcgc taccggcgat gccagttcgt agatttcagg
     1201 aacctcgtgc aggtggcccc caagtagcgt atccattcgc cctgcctgtg tcatgtcctt
     1261 cttcatatcc gcggcgccgg gcaatccgac ctccacttta ggcatgccga tcacccgttc
     1321 ctggttgtaa tgatggtagc atgcccgcaa atcggtcgga ccgtaatagg atacgaccgc
     1381 ccgcacagat agatcgcttt cctggacatc cgggggattc agcgaaggat ggtcgggcgt
     1441 gtaagccgcc agtaaagcca ggtggcctcc cgccgaggct cccccgagga cgattcgctc
     1501 ggggtccacc tgatatctgt cggcgttggc tttcatccag gccactgccc gtttgacgtc
     1561 cccgatcatt ccatagatat ccacttccgg acatagccgg taagccacgt ccatgatcac
     1621 gtgcccctgg gctgccaggt gccggaagag cgggcgcgta ccggagtcct tgtcaaggat
     1681 ccaccaggcg ctggcatgta gatagaccag ggccaggcca gagtagggga cgccatccgg
     1741 aggctgccac acgtcgcaca agagcttgcg gtctgtgccg gggatcgtcc agaagggaat
     1801 gtttcgctcc cagcgcggct ctcctgtccg cggccatccc aggttccagc gtcttttgag
     1861 catgtgggat gctcgggaag acgggattct ggccttccaa tcctttccaa aagcaaggtc
     1921 gaaatctggt tgggggacgg tcacgcgggc gatatagagt atggataccc ccgcgctcag
     1981 cacgccggct gccactggga tgggggtatg gtatagccag cccaatcccg cccctaacag
     2041 gctcaggacg accagaacag gagacaggac cacggcggcg atttggaacg cccaaaaata
     2101 ataattatag ggcggcttga gatgaataaa catcgatgcg tttagcacca aaggcaacaa
     2161 tgagagcaca tatgcaatga tgatcatgat ggctttacat cctcatcgga atcctgggcg
     2221 ggtggacagc cacaggtaac tgagatcagg gtcaggttga gatcgcggat gcgctcgagc
     2281 aagccgtgga gagcggcttg gtcgggcagc gtgccagaaa gcagggtttc ctctaattcc
     2341 agatgcgtca acttcaagcc tgcaaaccaa tccgaccaat gctcattcag atagcccttg
     2401 attttgatct cgtaatattc tggcatgtca gctctcctta tccgtatatg acaataattt
     2461 agcgcacaac gaggcagccc gcatctgcca aagggtatat tcggagttta ttttcggggt
     2521 ttagttggac gctagcactt cactccccct tccccacagg gaaagaggct gggggatggg
     2581 gtaataggcg accatagttt gccttggcgc taccagtcgt aaagttgatt atttgctagg
     2641 cggttgttgg agatcaggga aggatgccga gctgcctggc gcgcgccact gcctcggtcc
     2701 ggttggcgac gtccagcttg cgatagatac tggatgtatg agcttttaca gtacctgggg
     2761 aaacgaagag ctgcccggcg atctctcggt tggactttcc cagagccaat aactcga
//
LOCUS       DBNHOH010000909         5215 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_121902, whole genome shotgun sequence.
ACCESSION   DBNHOH010000909 DBNHOH010000000
VERSION     DBNHOH010000909.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_121902"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..805)
                     /locus_tag="AC16V7_16395"
     CDS             complement(197..805)
                     /locus_tag="AC16V7_16395"
                     /inference="COORDINATES: protein motif:HMM:NF024135.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HIR6258754.1"
                     /translation="MEKVLVAYTTHSGSTTEVAQVIGEELAKNGAVVEIYRLEEVTSL
                     DAYSAVVIGAPMILGWHRAAVKFVKRHQQALSERPVALFFMAMSLTQTGDTRVDQVPI
                     TVDSELAKLPANEGRLSFRERYATVTNYLRPVLRAAPSVKPVSVGFFGGKLEYFRLKL
                     FALLFVMLVIRAQPGDKRNWSLIREWAASLYPLLVGVRAGGT"
     gene            complement(838..2223)
                     /locus_tag="AC16V7_16400"
     CDS             complement(838..2223)
                     /locus_tag="AC16V7_16400"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017745774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent decarboxylase
                     family protein"
                     /protein_id="HIR6258755.1"
                     /translation="MEPSEFQILEKDSINVLRQALDKLEAGFTALPEVDASLKDDERV
                     ARLLLEVAERLQDNYPYFHPLYAGQMLKPPHPLARLAYMLAIWINPNNHALDGGRASS
                     AMEKEAVAEIARMFGWETHLGHLCGGGTMANLEALWVAGQLQPNSKIVASEQAHYTHK
                     RISAVLGLEFEATPCDRRGRMDVGALKRRLDQGGIGTVVATIGTTATGAVDTLPQLLG
                     LREQYGFRLHADAAYGGYFTLVDNLAAETRLSYERLGEVDSIVVDPHKHGLQPYGCGC
                     VLFRDPSVGRFYKHDSPYTYFTSTELHLGEISLECSRPGAAAVGLWATQRLLPLVHGG
                     EFAGMLHKTRAAALVLYERLMMDERFLTAFAPQLDIVIWGLRAARVSQASELARLVFA
                     EAAQHDLHLALAELPVEFFDLDTAGMERDRDTITCLRSVLMKPEHQEWVGRIWDILDE
                     ATSAVLARGLN"
     gene            complement(2237..2602)
                     /locus_tag="AC16V7_16405"
     CDS             complement(2237..2602)
                     /locus_tag="AC16V7_16405"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HIR6258756.1"
                     /translation="MKVASGVDLIEIERLEGTIQRHGDRFLKRVFTQQELDEAGQSMA
                     SLAARFAAKEAVAKALGTGIGTVAWQEIEVLRGEARQPVLHLHGEASRLAARLGLQDW
                     SLSLSHTHTHAIAMVVAVG"
     gene            complement(2619..3890)
                     /locus_tag="AC16V7_16410"
     CDS             complement(2619..3890)
                     /locus_tag="AC16V7_16410"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR6258757.1"
                     /translation="MMNSLHSLPGPDDITRHQLPNGIVLLTRPNFNSPSVAMTGYLPS
                     GAIFDPDEQLGLADFTAAALMRGTAQRDFQAVYDALESAGASLGFNGGTHTVSFGGKA
                     LAEDLDLLLDLVSESLRQPVFPDEQVERLRAQLLTGLQIRAQDTGEMASLAFDQIVYA
                     GHPYSRPEEGYIETVQAISRQDLVDFHLRYFGPRGMVIVVVGAVEPARVIAGVEKVFG
                     DWQNPQQPEQPSLPSLTSLQDNQTQRVTIAGKYQADIVMGVAGPPRRSPDFMAAYVGN
                     SVLGQFGMMGRIGDSVRERAGLAYYASSSISGGLGPGPWRVSAGVNPANVEKAIDLIR
                     QEIARFVSEPVLADELADSQANYIGRLPLTLESNGGVAGALLNLERYDLGLDYYRRYA
                     DLVREVTVDAVLETASRYLHPKRLGIAIAGP"
     gene            complement(3887..5128)
                     /locus_tag="AC16V7_16415"
     CDS             complement(3887..5128)
                     /locus_tag="AC16V7_16415"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR6258758.1"
                     /translation="MLKEIHTAPIISHWVWYRVGSRNEVAGFTGVSHWVEHMQFKGTP
                     QFPSTELDKANSRDGGFWNAITYLDWTTYFETMPAEKIDLALRLEADRMVNSQFDPQE
                     VASERTVIISERQGNENEPLFRLGEELQALAFRVHSYHHEVIGDLADLETMQRDDLYR
                     HYRQYYTPNNAVIAVAGDFESEVMLGRIRELFEPIPAGEALLQRIRPEPAQSGERRFT
                     LEGPGETTYIQFAHRAPAASDPDFFPLTVLDSLLTGPSSLNMFGGGISNKTSRLYRAL
                     VEGELAVSVYGGLQATIDPFLHTITIYVRPGCSVETVFSKLEDEIKRLQDAPPSPDEL
                     ARAVKQARALFAYGSESITNQAFWMGFSEMFASYDWFTGYLDNLAAVTPGDVQRMAQV
                     YLRPQNRIHGIYLPTGDGEVS"
BASE COUNT         1164 a         1598 c         1392 g         1061 t
ORIGIN      
        1 cgagcaaatc cagctcgcaa ctcatcactt ccaccaaaac ctttatagca tattcggata
       61 tcaaagtcaa cgcttattca gcttacataa atctgaaagt cagctatctt aacagggaca
      121 aatgccaaga tgaaaaggac gctattcaag catccttgag gcacctctat atttgagaat
      181 cttggagcag cgcgcgctaa gtgcccccgg cacgcactcc aaccaacagt ggatataaac
      241 tcgccgccca ttcccgtatg agcgaccagt tgcgcttgtc ccccggctgc gcccggatca
      301 ccagcataac gaacaacagc gcgaacagct tcaggcggaa gtactccagc ttgccgccaa
      361 agaagccaac gctcaccggc ttgaccgacg gggctgccct gagcactggg cgcaggtagt
      421 tggtcaccgt ggcataacgc tccctgaagc tcaagcggcc ttcatttgca ggcagtttgg
      481 ccagctcact gtctaccgta attggcacct gatccacgcg ggtatcaccc gtctgggtga
      541 ggctcatcgc catgaagaac aaggcgaccg gtctttcact gagcgcttgc tggtggcgtt
      601 tgacgaactt gaccgccgcc cggtgccatc ccaggatcat cggcgcgcca atcaccaccg
      661 ccgagtaagc atctaggctg gtcacctctt cgaggcgata aatctccacc acggcaccgt
      721 ttttcgctaa ctcctccccg atgacctgcg ccacctcggt tgtcgatccg ctgtgggttg
      781 tgtaagcaac caatactttt tccatgcaac cacccctttc tcatccttgt tagctggtta
      841 atttaagcca cgtgccaaga ccgcacttgt agcttcatcc aaaatatccc agattcgccc
      901 cacccattct tggtgctccg gcttcatcaa aactgaacgc aaacaggtga tcgtatcccg
      961 atcccgctcc attccggctg tgtctagatc aaagaactcg actggcagct ctgccagcgc
     1021 caggtgcagg tcgtgctgcg cagcctcggc gaataccaaa cgcgccaatt cagaggcttg
     1081 gctgacacga gcggcgcgca gcccccagat cacgatatcc aattgcggcg caaaagcggt
     1141 tagaaagcgc tcgtccatca taagtctttc gtagagcacg agagctgcgg cgcgcgtctt
     1201 gtgaagcatg cctgcaaact cacccccgtg gacaagaggg agcaggcgct gcgttgccca
     1261 cagtccaacc gcggctgcgc caggccggga gcactccagg ctgatctcac ccaaatgcag
     1321 ctctgtcgaa gtgaaatatg tatacggcga atcatgctta tagaagcggc ctacggatgg
     1381 atcgcggaaa agcacgcagc cacagccata tggctgaaga ccatgcttgt gcgggtcgac
     1441 gacaatggaa tctacctcgc caagccgctc gtagctaagg cgcgtctcgg ctgccaggtt
     1501 atcaactaag gtaaagtagc ctccgtaggc tgcatccgca tgcaggcgaa aaccgtattg
     1561 ctcgcgcagc ccaagcaatt ggggcaaggt gtcgactgcg cctgtggcgg tcgtcccgat
     1621 ggtcgccacc actgtgccga tgccaccctg atccagacgg cgcttcaatg cgcccacatc
     1681 catgcgccct cgccggtcgc agggggtggc ttcgaattcc agcccgagca cagcgctgat
     1741 gcgcttgtgc gtatagtgcg cctgctctga agccacaatc ttggaattgg gctgaagctg
     1801 tcccgccacc catagcgcct ccaggttagc catcgtgccc ccgccgcaca ggtgccccag
     1861 gtgcgtctcc cagccgaaca tgcgggcgat ctcagccact gcttccttct ccatggctga
     1921 gctggcgcgc ccgccatcca gcgcatggtt gttcgggttt atccagatcg ccagcatgta
     1981 tgccaggcga gccagaggat gaggcggttt gagcatctgc ccagcgtaca gcggatggaa
     2041 atagggataa ttgtcttgca ggcgctcagc gacttccagg agcaagcgcg ccacccgctc
     2101 atcatctttt agcgatgcat ccacctcagg cagagcagta aatcctgcct ccagcttatc
     2161 aagcgcctgg cgcagtacat tgatcgaatc cttttccagg atttgaaatt cagagggttc
     2221 cataagcgta atccgtttac cccaccgcga caaccatggc gatagcatgc gtatgggtgt
     2281 ggctcaggct gagtgaccaa tcctgcaggc ctagcctggc agctaggcgg gacgcctcgc
     2341 catgcaggtg caaaaccggc tggcgggcct cgccacgcaa cacctcgatc tcttgccagg
     2401 cgaccgtacc gatgcccgtc cccagggctt tagcgaccgc ctctttggca gcaaaacgcg
     2461 cagccagcga ggccatactc tgcccggcct catccaactc ctgctgggtg aagacccgct
     2521 tgaggaatcg atctccatgg cgctggatgg tgccctccag acgctcgatt tcaatcaggt
     2581 caacaccgct agcaaccttc atctcaacct gctactgctc aagggccggc aatggcaatc
     2641 cccaagcgct tagggtgcag gtagcggcta gcggtttcca ggactgcatc taccgtaact
     2701 tcccgcacca ggtcggcgta acgccggtag tagtccagtc ccaagtcata acgctccagg
     2761 ttgagcaatg caccagccac accaccgttt gattccagtg tgagcggcaa gcgaccgata
     2821 tagttcgctt ggctatctgc caactcgtct gccaataccg gctcactgac aaagcgggcg
     2881 atctcctggc ggatcaggtc aatggctttc tcgacattag ccgggttgac accagcagaa
     2941 accctccaag ggccgggtcc caaaccgccg cttatgctgc tggaggcata atacgccagc
     3001 ccagctcgct cacgaacgct atctccgata cgccccatca tgccaaactg tcccagaacg
     3061 ctgttgccca cataagccgc cataaagtcg ggggagcgtc gcggtggccc agcgacgccc
     3121 atcacaatat cagcctggta tttcccagca atcgtgactc gctgagtttg attgtcctgc
     3181 agggaagtca aggatggtag tgacggctgc tcaggctgct gcggattttg ccaatctcca
     3241 aagaccttct cgacccccgc aatgacccga gcaggctcca ccgcgcccac caccacgatg
     3301 accatgccgc gcggtccaaa atagcgcaag tggaaatcga ccaggtcctg gcggctgata
     3361 gcctgtactg tctcaatata accttcttct gggcggctgt atgggtgccc agcatagacg
     3421 atctggtcga acgcaaggga tgccatctcg ccggtatctt gagcgcggat ttgcaacccg
     3481 gtgagtagct gcgctcgcag ccgctcaacc tgctcatccg gaaaaaccgg ctggcgtaga
     3541 gattcagaaa caagatccag caacaaatcc agatcctctg ccaaagcctt acccccaaag
     3601 gagaccgtat gggtgccccc attgaagcct aggcttgctc cggcggattc gagggcatcg
     3661 taaacagctt gaaagtcacg ctgagcagtg ccgcgcatca gggctgccgc agtgaaatcc
     3721 gccaagccga gctgttcatc tgggtcgaaa atagcgccgc tcggcaaata gccggtcatg
     3781 gcgacggagg ggctgttgaa attcgggcgc gtcagcagta cgatgccatt gggcagctgg
     3841 tggcgggtga tatcatcagg gccgggaagt gaatgcaggc tgttcatcat gacacctccc
     3901 catcacccgt cggaaggtag ataccgtgaa tacgattttg ggggcgcaga tagacctgcg
     3961 ccatgcgttg cacatcgcca ggcgtaaccg ctgccaggtt atccagatat cccgtgaacc
     4021 aatcgtaact ggcaaacatc tcagaaaatc ccatccagaa tgcctggttg gtaatggatt
     4081 cgctgccata ggcaaacagg gcacgcgcct gcttgacagc gcgggctagc tcgtcaggag
     4141 aagggggagc atcttgcaag cgcttgatct catcctctag cttggagaag accgtctcca
     4201 ccgaacagcc agggcgtacg tatattgtta tggtatgcag gaatgggtct atcgttgctt
     4261 gcagcccccc atacacgcta accgccagct ccccctctac cagggcgcgg taaaggcgcg
     4321 acgtcttgtt cgagatgccc cctccgaaca tattgaggct gctagggccg gtaagcaggc
     4381 tgtccagcac agtgagcgga aagaaatcgg ggtccgatgc cgctggggca cggtgggcaa
     4441 actggatgta ggtggtctcg cccggacctt ccagggtgaa gcggcgctca cctgactggg
     4501 ccggctccgg acggatacgc tggagcagcg cctcaccagc agggattggt tcaaacaact
     4561 ctcggatgcg tcctagcatc acctcgcttt caaaatctcc ggcaacggca atcacggcat
     4621 tattaggagt gtaatattgc cggtaatgcc ggtataggtc atcgcgctgc atagtctcca
     4681 ggtcagccag atcaccgatc acctcgtggt gataggaatg cacccggaaa gctaatgcct
     4741 gcagctcctc gcccagccga aaaaggggct cgttctcgtt gccctgccgc tcggaaataa
     4801 tcactgtgcg ctcagaggcg acttcttgcg gatcaaactg gctgttaacc atccggtcag
     4861 cctccaggcg caaggccagg tcgattttct cagcgggcat tgtctcgaag tacgtggtcc
     4921 agtccaggta agtaattgcg ttccaaaaac ctccatcgcg tgagttcgcc ttatccagtt
     4981 cggttgaggg gaattgcggt gtccccttaa attgcatgtg ctcaacccag tgcgagaccc
     5041 ccgtgaaacc cgccacttcg ttccgggagc caactcgata ccatacccaa tgactgatga
     5101 ttggagcggt atggatttct ttcagcataa cttgtattcc attagaaagc tgcgtcttcg
     5161 tgaaatttgt tcccactatt acctctcgca aaacattgtg agcgcctgga acgag
//
LOCUS       DBNHOH010000910         2324 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1789377, whole genome shotgun sequence.
ACCESSION   DBNHOH010000910 DBNHOH010000000
VERSION     DBNHOH010000910.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1789377"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="AC16V7_16420"
     CDS             <1..449
                     /locus_tag="AC16V7_16420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258759.1"
                     /translation="RQAQDPAGNQPLDTLARWRLQFLAASIASQVPVDLALLAEFPAQ
                     DDPISAYFPAVDQAIIALDESVAVVERQLTDLTAQREAMLEEWKIESQASHNLTAHLT
                     IEPISIENSSARPVRSTSQMALVGGSLGLLLWALVWLARPLYKAGR"
     gene            446..1921
                     /locus_tag="AC16V7_16425"
     CDS             446..1921
                     /locus_tag="AC16V7_16425"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR6258760.1"
                     /translation="MSLASWSPLANKTVRRLQKIAWALFLVFLPVTSFPLFPKVMGGS
                     ALVRPLSLFPLILLVLLVTLPHLVRRPLPRTLLILIPFILVAVASSLMSTLSGIETTS
                     SVTPFGRILRALFTLGIGTAMYFTVSLYSSKEDQLRASLRWLYIGFSVALLWGSLQAV
                     YIIQFDSAYFHSLAKLQSFISSRKLFTTRVSGMTYEPNWFAEQISFLLMPWLLASVLS
                     GYSAFRWRWRWLTIEWFLLLWAMAVLIFTFSRAGLAILFLLAFTGFLFFRGQRDSKPD
                     TQLRGRRLWVRRLLEAGVVVAALIGLIFFASLKNSFFARIWSYWRDVKNASLEGYFEY
                     LGFGARFRYSDTAYRIYEAHPVLGVGLGNYAFYFPDMMPDEPLATSPELLRLIVPEGN
                     RERLITPKNFYLRLLAETGLVGMAAFAAFFAAVFGCALFLWLSSDREQRFWGRAGLLG
                     LIAFLPSAMSFDSFAIPNMWVVFGLITSAAWVFSKKETSNS"
     gene            1918..>2324
                     /locus_tag="AC16V7_16430"
     CDS             1918..>2324
                     /locus_tag="AC16V7_16430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258761.1"
                     /translation="MNRPWIITVVIAILVLLVICCICLVLTFGAGALLFLNVESSRSV
                     NVIEPTPLITQVVDTPTVEAFSTPRPTKAPPSPEPEQPAPETKPTPSTPAIAASDPQT
                     TLKILSDTLVPINDPADLAKRLEGKGDIPPTLPP"
BASE COUNT          429 a          709 c          618 g          568 t
ORIGIN      
        1 ggcggcaggc gcaggatcct gccggcaacc agccgctaga taccttagcc cgctggcggc
       61 tgcaattcct ggcagccagc attgccagcc aggtgccggt cgatctggcc ctgcttgcgg
      121 aattccctgc gcaagatgat ccgatcagcg cctatttccc cgccgtagat caggcgatca
      181 ttgccctgga tgaaagcgta gcggtcgtcg agcgacaatt aaccgacctc acagcacagc
      241 gcgaagccat gctggaagaa tggaaaatcg aatctcaagc ctcgcacaac ctgacggccc
      301 acctcacgat cgagccaatc tccatagaaa atagttccgc caggccagtg cgcagcacca
      361 gccagatggc cctggttggc ggctcactcg gcttgctgct ctgggcgctc gtctggttgg
      421 cacggcccct gtacaaggcg ggaagatgag cctggcatct tggtccccgc tcgccaacaa
      481 aaccgtgcgc agattgcaga agatcgcctg ggcgctgttc ctggtcttcc tgccggtcac
      541 cagcttcccg ctctttccga aggtcatggg cggaagcgcc ctggtgcgcc ccctatcgct
      601 cttcccgctg atcttgttgg tcctgctggt gacgctgcca cacctggtcc gcagacccct
      661 gccacgcaca ctgctcatcc tcatcccctt tatcctggta gctgtggcaa gctcgctgat
      721 gtcgaccctg agcggcattg agacgacctc cagcgtgacg ccctttgggc gcatcttgcg
      781 agcgctcttc actctgggca tcggcacagc catgtacttt acggtttcgc tatactcaag
      841 caaggaggat cagttacgcg cctcgctgcg atggctgtat atcggctttt ctgtggcact
      901 gctgtggggt tctttgcagg cggtgtatat aatccagttc gactccgcct atttccattc
      961 cctggcgaaa ctacaatcct ttatctcgtc gcgtaagctg ttcacaaccc gcgtatcggg
     1021 tatgacctat gagccgaact ggttcgccga acaaatcagt ttcctgctga tgccatggct
     1081 attggcttcg gtgctgtcag gctacagtgc cttccgctgg cgctggcgtt ggcttaccat
     1141 cgaatggttc ctgctcttgt gggctatggc tgtgttgatc tttacttttt cacgcgctgg
     1201 cctagccatc ctcttcctgt tggcattcac tggcttttta ttcttccgcg ggcagcggga
     1261 ctcaaagccc gatacacagc tgcgcgggcg caggctctgg gtgaggcgcc tgttggaggc
     1321 cggtgtagtg gtagcggctt taattggcct gatatttttt gccagcctga aaaactcttt
     1381 cttcgctcgc atttggagct actggagaga tgtcaagaat gccagtctag aaggttattt
     1441 tgaatacctg ggattcgggg cgcgctttcg ttacagtgac accgcctacc gtatctacga
     1501 ggcgcaccct gtgctgggag ttggcctagg gaattacgcc ttctacttcc cggatatgat
     1561 gcccgatgaa cccctggcga cctcacccga gctcctgcgg cttatcgtcc ccgaaggcaa
     1621 ccgcgagcga ttgatcacac ccaaaaactt ttacctgcgc ctgttggccg aaaccgggct
     1681 ggtcggcatg gcggctttcg ccgctttctt tgcagcggtt ttcggctgcg cgctctttct
     1741 atggctttca tcagatcggg agcaacggtt ttggggcagg gccggcctgc ttggattgat
     1801 cgcctttttg ccgtcagcaa tgtcttttga ttcgtttgcc ataccgaata tgtgggtggt
     1861 gtttggcttg atcacctccg cggcctgggt attctcaaaa aaggaaacat ctaattcatg
     1921 aatcgtccct ggattatcac tgtcgtcata gcgatcttgg ttttattggt aatttgctgc
     1981 atctgcctgg ttctgacatt tggcgccggt gcattgcttt tcttgaacgt tgaatcttcc
     2041 cgtagtgtca acgtgataga gcctacgccg ctgattaccc aagtagttga cacaccaacc
     2101 gtcgaggcat tttccacgcc tcgtccgaca aaagcgcccc cctccccaga acccgagcag
     2161 cccgcacccg agacaaagcc aaccccaagc acacctgcca tcgcggccag cgaccctcaa
     2221 accacgctca agatcttgag cgataccctg gtgcccatca acgatccggc agatctggct
     2281 aagcgcctgg agggcaaggg ggatatccca cccaccctgc cgcc
//
LOCUS       DBNHOH010000911         3601 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_442372, whole genome shotgun sequence.
ACCESSION   DBNHOH010000911 DBNHOH010000000
VERSION     DBNHOH010000911.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_442372"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..1231
                     /gene="mtnA"
                     /locus_tag="AC16V7_16435"
     CDS             188..1231
                     /gene="mtnA"
                     /locus_tag="AC16V7_16435"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0033353 - L-methionine cycle [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HIR6258762.1"
                     /translation="MRTVEWDYQRNQVRMIDQRLLPGEFRVVSFADHSQVAGAIRDMV
                     VRGAPAIGATAAFGLALAAHNSIAAESAALRADLKQAAEVLHEARPTAANLDWALQRV
                     LAVILKTQGNPNDLRAAALSEAQRIADEDVEINQQMARYGAELVADGDTIIHHCNTGA
                     LATVDWGTALGVIRMAHEQGKRIHVLVDETRPRLQGARLTAWELEQYGIPYEIIGDNA
                     AGYFLQSGQVQKVLFGADRMAANGDVANKIGTYMLALAAYDNNVPAYAVVPTSTIDLS
                     LAHGGLIPIEERSAAEVLSLQVGGQPVTPTGAAARNPAFDVTPHRLLTAIVSEKGVIY
                     PPFEENLRKSLKE"
     gene            1359..2327
                     /locus_tag="AC16V7_16440"
     CDS             1359..2327
                     /locus_tag="AC16V7_16440"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR6258763.1"
                     /translation="MDILLTGSVAFDYLMIFPGYFREHILPDKLDSISLSFLVESMVR
                     LRGGTAPNIAYTLALFGQRPRLFATVGEDFEEYRIWLESKGVDTTWAKVIPGVYTASY
                     FANTDRSNAQIASFYPGAMAYAAQLSLHELSNNPPDLVIISPNDPGAMNCYVDECLEM
                     GIPYLYDPSQQIVRMSKDELSRGIQGAMAVFVNDYEFALIQKMTGKTPSDIMERAKFL
                     VVTRGEQGATIYSGDEEYHIPVVPPSQISDPTGVGDAFRGGFLTAYSHSLDYLTCGQM
                     GSLAATYCLEQHGPQGHSYTPAEFVARFRQHFDDQGKLDVLLGDEG"
     gene            2386..>3601
                     /gene="ahcY"
                     /locus_tag="AC16V7_16445"
     CDS             2386..>3601
                     /gene="ahcY"
                     /locus_tag="AC16V7_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HIR6258764.1"
                     /translation="MDNYDIKDTKLAEGGRRKIDWAAREMPVLRLIRERFEKERPLAG
                     MRIAACLHVTTETANLMQALHAGGADIVLTASNPLSTQDDVAASLVTHFEIPVYAIKG
                     EDNVTYYRHINAALDHKPHMTMDDGADLVGTLHKDRRELLGNMIGGTEETTTGVIRLR
                     AMAADGALVFPVIAVNDAMTKHFFDNRYGTGQSTIDGIIRATNTLLAGKAFVVAGYGW
                     CGRGLAMRARGMGANVIITEVDPLPALEAVMDGFRVIPMSEAAEIGDIFCTVTGDINV
                     IDSQHFEVMKDGAIVANSGHFNVEINIPALESMAVDKRLVRPFVEQYALSDGRRINLL
                     GEGRLINLAAAEGHPPSVMDMSFANQALAAEYMVKNVDKLEKKVYAVPEEIDKEIARI
                     KLESMGVKIDVLT"
BASE COUNT          833 a          919 c         1005 g          844 t
ORIGIN      
        1 actagccacc cctcatatca agcccttttg tggggagtcg ggcaggtcat gacctgctca
       61 actccccact tatttattac acaccacatt gatgattctt tgacaatcta ccatttgcat
      121 gttaaactca cgtctgctgt ccagccgtga gtaattacgt gtcaccccac tgctaacttg
      181 gaccataatg cgtactgttg aatgggacta ccagcggaac caagtccgca tgatcgatca
      241 gcgtctgctc ccaggcgagt tccgcgtcgt atctttcgcg gatcatagcc aggtggcggg
      301 ggcaatacgt gatatggttg tgcgcggtgc gccggcaatt ggtgcaaccg ctgcctttgg
      361 gttggcactc gctgctcata attcgattgc cgcggaatct gccgcccttc gtgctgacct
      421 gaaacaggca gccgaggtgt tgcacgaagc ccgcccgaca gctgccaatc tggattgggc
      481 tttgcagcgc gtgttggctg tcatattgaa aacccagggc aatcccaatg acttgcgagc
      541 tgctgccctt tcggaggccc agcgtattgc tgacgaggac gtagaaatca accagcagat
      601 ggcgcgctac ggtgcggagc tggtcgctga tggcgatacc atcattcatc attgcaatac
      661 cggcgcgttg gctactgtcg attggggcac cgcgttgggc gtgatccgta tggcgcacga
      721 acagggaaag cgcatccatg ttctggtgga tgagactcgc ccgcgtttgc aaggggctcg
      781 cctgacggct tgggagttag aacaatatgg tatcccgtat gagatcatcg gcgacaacgc
      841 cgcgggttat tttttgcaat ctggacaggt gcagaaggtt ctatttggag ccgaccgtat
      901 ggctgcaaat ggcgacgtgg caaataaaat cggcacgtac atgttagcgc tggcagctta
      961 tgacaataac gtccccgctt atgcagtagt gcctacctcc accatcgatc tgtctctggc
     1021 gcacggcggc ttgataccga tagaggagcg tagtgctgcg gaagtgttaa gcttgcaggt
     1081 aggtggtcaa cctgtgacgc ccacaggtgc agcagcccgc aatccagcat tcgatgtcac
     1141 cccgcaccgc ctgctcactg ccattgtttc cgagaagggt gtcatttacc cgcctttcga
     1201 agagaatcta cgcaagtctc tgaaggagta gataaaggag taggttcgga agcaaaagga
     1261 caggtttcac gtagcatgag aggatatcga agaagactcc atgaaaatcc agcaatccag
     1321 gagtcaattc tggctgtctt actaatacaa gcatgaagat ggacatttta ctcacgggat
     1381 cggtagcctt tgactacctg atgatatttc ctggctactt tcgcgagcac attttgccag
     1441 ataagctcga ttctatcagc ctgtcctttc tcgtcgagtc gatggtgcgc ttgcgcggtg
     1501 gaaccgcgcc taatattgct tataccctgg cgctttttgg acagagaccg cgcctgttcg
     1561 ccacggttgg cgaggatttt gaagaatacc gcatctggct ggagagtaaa ggggtagata
     1621 caacctgggc taaggtcatt ccaggggtgt acaccgcatc ttattttgcc aataccgatc
     1681 gctctaacgc ccagattgcc agtttctatc cgggcgcaat ggcatacgca gcgcagctat
     1741 ctttgcacga gctcagcaac aatccacctg acctggtgat tatctcgcct aacgatcctg
     1801 gtgccatgaa ttgctatgta gatgagtgcc tggagatggg tattccctac ctgtatgatc
     1861 caagccagca gattgtgcgt atgtcgaaag acgaactcag ccggggcatc cagggcgcaa
     1921 tggcggtctt cgtaaatgat tatgagttcg ctttgattca aaagatgacc gggaaaaccc
     1981 cctcggatat catggaacgc gccaaatttc tggtggtaac gcgtggcgag caaggcgcaa
     2041 ctatctacag cggggatgag gagtatcata ttccagtggt tccaccttcg caaatttctg
     2101 atcccaccgg tgtgggggat gccttccgag gtggcttcct gactgcttat agccatagtc
     2161 tggattatct cacttgcggg caaatgggat cattagcggc aacatattgt ttagagcaac
     2221 acggccctca agggcattcc tacacgccag ccgagtttgt tgcccgcttc cgccagcatt
     2281 ttgacgatca gggtaagctg gatgttctgt tgggggatga gggatagggt atataattca
     2341 tcctggccct cactgaatgg gaattttttc ttacaggaga aacgcatgga taattatgat
     2401 attaaagata ccaaacttgc ggagggaggc cgtcgcaaga tagattgggc tgccagagaa
     2461 atgcctgtgt taaggttgat tcgcgagcgt tttgaaaaag aacgcccgct ggcggggatg
     2521 cgcattgccg catgcctgca cgtgacaacc gaaacagcca acctgatgca agccctgcat
     2581 gcaggtggtg ccgacatcgt cttgactgct tccaacccgc tgtcaaccca ggacgacgtg
     2641 gctgcttccc tggtgacaca tttcgaaatc ccggtatacg ccattaaagg ggaggacaat
     2701 gtcacctact accggcatat caacgctgct ctggaccata aacctcatat gaccatggac
     2761 gatggcgctg acctggtggg gactctacat aaagatcgcc gcgagttact tgggaatatg
     2821 atcggcggca ccgaagagac cacgactggg gtgatccgtc tgcgtgccat ggctgcagat
     2881 ggtgccctgg ttttcccggt catcgcggtc aacgacgcca tgaccaagca tttcttcgat
     2941 aaccgctatg gaaccgggca atcgacgatt gatggcatca tccgcgccac caatacgctg
     3001 ttggctggca aagcatttgt tgtggcaggc tatggctggt gcgggcgcgg tctggctatg
     3061 cgtgctcgcg gcatgggcgc caatgtaatc attacggaag tagatccgct acctgcacta
     3121 gaggccgtca tggatggttt ccgggtcatc cctatgagtg aagcagccga aattggggac
     3181 atcttctgca ccgtcactgg agacatcaac gtcatcgaca gccagcactt cgaagtgatg
     3241 aaggatggag ccattgtggc taactcgggc cattttaatg tcgagataaa tatccccgcc
     3301 ctggaatcga tggcagtgga caagcgcctg gtgcgaccct tcgtcgagca gtatgcactg
     3361 tccgatgggc ggcgtatcaa cctgttgggg gaagggcgcc tgatcaacct ggcagccgct
     3421 gagggtcacc cgcccagcgt gatggacatg tcctttgcca atcaggcact cgcggcagag
     3481 tacatggtga aaaatgtgga caaacttgag aaaaaggttt atgccgtgcc ggaagagatt
     3541 gataaagaaa tcgctcgcat caagctggaa tcgatgggcg tgaaaatcga cgtgctgact
     3601 g
//
LOCUS       DBNHOH010000912         2973 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1147948, whole genome shotgun sequence.
ACCESSION   DBNHOH010000912 DBNHOH010000000
VERSION     DBNHOH010000912.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1147948"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="AC16V7_16450"
     CDS             <1..202
                     /locus_tag="AC16V7_16450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258765.1"
                     /translation="VEPDLTIQLPDGSTRQYYMYPTGNDGQTRMTIEPIDAPGGTLIP
                     YKVCIYGLGGQAYCTQDSFLIW"
     gene            491..991
                     /gene="sodN"
                     /locus_tag="AC16V7_16455"
     CDS             491..991
                     /gene="sodN"
                     /locus_tag="AC16V7_16455"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HIR6258766.1"
                     /translation="MIFKVFEALDKRFHFERASAHCDIPCGIYDPSPAQIAALTVVRM
                     VDLMADLEAKGGEKDRAYTNSMGRYVAVKEEHAEKAKHEIRIIWGDYIKPQHMEKHPQ
                     LHDLVHKIMQYGSKSRQTADRDAAVQFVDAINQFSEIFWDTKGVKTKRAKAPYNPPLE
                     MVYPDL"
     gene            991..1293
                     /locus_tag="AC16V7_16460"
     CDS             991..1293
                     /locus_tag="AC16V7_16460"
                     /inference="COORDINATES: protein motif:HMM:NF021981.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HIR6258767.1"
                     /translation="MLRVIQVTGDSLSPEYREGDYVVITTIPFFLNRIQVGDVIVFQQ
                     PPFGTMIKKVERILGLGDGFYVSGTHQQSVDSRRYGSIPREALVGKVIWHILKPSR"
     gene            1343..2230
                     /locus_tag="AC16V7_16465"
     CDS             1343..2230
                     /locus_tag="AC16V7_16465"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR6258768.1"
                     /translation="MIFGILTISTGSIMVRYAQSYAPSLVVAALRLFLATLVLTPFAL
                     SRHRSELAALSRGELALAGLSGFFLALHFAFWIISLEYTNVASSVVLATSTPLYVALF
                     APFTIKERVSRIILLGVALALVGGVVVGLSDACSINQAGLQCPELGQFFRGKAIIGDI
                     LATMAAVVAAAYVLIGRRLRAKLSLIGYISLVYGVSAIVLVVVMFASGNSPFGFPPMA
                     YFWFLMLALVPQLLGHSSFNWALGYASAALVSVALLVEPIGATLLAYIFLGETPTMLK
                     IFGAILVLIGVYIASRGEK"
     gene            2331..>2973
                     /locus_tag="AC16V7_16470"
     CDS             2331..>2973
                     /locus_tag="AC16V7_16470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258769.1"
                     /translation="MKNPNPAPNTQTAPTESTGMVWSRFRRFAWDIAGVATLAFALMT
                     LLALMLPQLTSGVLLAWWADVLWLWFGWGSLWVVVLLTVAGLLLLRRTTLAKNGEMLR
                     LNWGRVIALEMVAFASLAWLAAWGGSSFARADVGLDGGRIGWALSYLLGELLSPLGMS
                     GSIWQMLILSGVMIVGVILGFGLNRQLAAWVDQQVATSQYSQADELGGEGAVSV"
BASE COUNT          637 a          717 c          815 g          804 t
ORIGIN      
        1 tgttgagcca gacctaacca tacaactgcc tgatggcagc accaggcaat attacatgta
       61 cccaactggc aacgacggtc agactcggat gactattgaa ccgatcgacg cgccgggcgg
      121 cacattaatc ccttacaagg tgtgcattta cgggctcggt ggacaagctt actgcactca
      181 agatagcttc ctcatttggt gatatcccta gaccgcgaaa atttctaaat cctttttcac
      241 tatattcccc tcgctcctcg caggggaata tttttaattt gcatgcttcc caaggttcga
      301 aaacttgaac ttcaaaacca ataagccagc caccagctct tccgtgtgct atcgaccttg
      361 tcaaaactct atgactcaga caattaatct tgacaggaga ggtctttatc attatgatca
      421 tttgtgtctt ctaccgtgta gaagtatcca aaataatgta acaagataat cacttatgaa
      481 ggaggattgt atgattttca aggttttcga agctttagac aagcgatttc attttgagcg
      541 cgccagcgcc cactgtgaca tcccctgcgg catttatgac cccagccctg cacagatcgc
      601 tgcgctgacg gtagtaagaa tggtggattt aatggccgac ttggaagcta agggtggtga
      661 aaaggatcga gcatacacta atagtatggg ccgttatgtt gcggtaaaag aagagcatgc
      721 tgaaaaggcc aagcacgaga ttcgtattat ctggggtgat tacatcaaac ctcagcatat
      781 ggagaagcac ccccagctgc acgacctggt gcacaaaatt atgcagtatg gctcgaaaag
      841 ccgccagacc gctgatcgcg atgcagcagt ccagttcgtt gatgctatca accagttttc
      901 agagatcttc tgggatacca aaggcgtgaa aaccaagcga gccaaggctc cttataatcc
      961 gccattagag atggtttacc ctgatctgta atgcttcgcg tgatccaagt gacgggcgat
     1021 agcctgtcac cagaataccg agaaggggat tacgtggtaa tcaccacgat ccccttcttt
     1081 ttgaatcgaa tccaggtggg tgatgtgatt gtttttcagc agcctccctt cggcacaatg
     1141 atcaaaaaag tagagcgcat cctgggattg ggggatggtt tttatgtatc cggcacgcat
     1201 cagcaaagtg tggatagccg gcgatacggg tcgatcccgc gagaagccct ggtgggcaaa
     1261 gtgatttggc atatcctgaa accatctaga taacatgcag cccccatcga gtcgacaccc
     1321 tccgctacct cccatggcgg tgatgatttt tggtatcctg acgatttcca ccggctcgat
     1381 tatggtccgg tatgctcaga gttatgcgcc ttcgttggtt gttgctgcct tacgcctatt
     1441 cctggctacg ttggtattga ctcccttcgc gctatcacgt caccgtagcg aacttgcagc
     1501 cctgtcgcgc ggcgagttag ccctggcagg cttatccggc tttttcttgg cgttacactt
     1561 cgctttctgg attatctcgc tggaatatac taatgtcgct agctcggttg tgctggctac
     1621 cagcacgccg ctgtatgttg cgctgtttgc cccatttacg ataaaagagc gcgtcagccg
     1681 cattatcctg ttgggcgtcg cactggcgtt ggtaggtgga gttgtggtgg gattaagcga
     1741 tgcctgtagc atcaaccagg caggcttgca atgccctgag ctggggcagt tcttccgtgg
     1801 aaaagccatc atcggtgata ttctcgccac catggcagcg gtggtagcag cggcatatgt
     1861 gttgattggg cgacgactac gtgcgaaact gtctctcatt ggctatatca gcttagtgta
     1921 cggcgtgtcg gctattgtgc tggtagtggt tatgtttgcc agcgggaact cgccatttgg
     1981 cttcccgcca atggcttatt tctggttctt aatgttggcg ttggtgccgc aattgctggg
     2041 gcattcctcc tttaactggg cgctcggata tgcctctgct gccttagtct ccgttgcctt
     2101 gctggttgag ccaattggcg ctacgctcct ggcgtatatc ttcctcgggg agacgccgac
     2161 gatgctcaag atatttggtg ctatacttgt tttgatcggt gtctatatcg cctccagagg
     2221 tgagaaatag ccatcgattg gcatgacaac agtcggagat ggagcattaa agtgaggaaa
     2281 cattatccta ctctggcccc aatcccctac ctggcatgtg ggcgagagcg atgaaaaacc
     2341 ccaaccctgc cccgaatacg caaactgcac caacggaatc aaccgggatg gtatggtcac
     2401 gcttccggcg ttttgcctgg gatatcgcag gtgtggcgac tttagccttt gctttaatga
     2461 ccttgctggc gctgatgttg ccacaactga ctagcggtgt attgctggct tggtgggcag
     2521 atgttttatg gttgtggttc ggttggggga gcctttgggt ggtcgtgctc ttgacggtgg
     2581 ctggattgct cttgctgcgg cgcaccactc ttgcgaagaa tggcgagatg ttgcgactga
     2641 actgggggcg tgtcatcgct ctggaaatgg tcgccttcgc cagcttggct tggttggctg
     2701 catggggtgg ttcatctttt gcgcgtgcgg acgtcggttt agatggtggg cgcatcggct
     2761 gggctctctc ctacttgctg ggagagctcc tttcgccgct gggcatgtct ggctcgatat
     2821 ggcaaatgct gatcttaagc ggtgtgatga tcgttggggt cattttgggt tttggattga
     2881 acaggcaatt ggctgcttgg gtggatcaac aagtagctac gtctcaatac tcccaggcgg
     2941 atgagctggg tggcgaggga gcggtgagcg ttg
//
LOCUS       DBNHOH010000913         3355 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1212236, whole genome shotgun sequence.
ACCESSION   DBNHOH010000913 DBNHOH010000000
VERSION     DBNHOH010000913.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1212236"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="AC16V7_16475"
     CDS             <1..644
                     /locus_tag="AC16V7_16475"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014305.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR6258770.1"
                     /translation="QVIHVEDWVIRQRVPAGEGPFPTIIMLHGWTGDENVMWIYATRM
                     PKNALLLAPRGLYSTPLGGHGWHPYKAKVWPLMDDFRASNEAFLELLNINNFPLVDFS
                     RLHFVGFSQGAAMSYAFALENGDRLSSVVGLSGFLPIGADAAATNESLHHKPIFIAHG
                     NQDFLVPVYRARETVNLFQKAGADVTYCEDDVGHKLSANCFRGMEAFFVKQGF"
     gene            complement(677..1804)
                     /gene="alr"
                     /locus_tag="AC16V7_16480"
     CDS             complement(677..1804)
                     /gene="alr"
                     /locus_tag="AC16V7_16480"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR6258771.1"
                     /translation="MNQGILSWAEIDLNAITFNVKALQRHVGRAVEIIAVVKANAYGH
                     GAVAVARTTLEAGATRLAVHRAIEGIELRQAGITAPILIMGYTPPDGAELIARWKLTP
                     SLTTSESALALSAQAISLGASIPVHIKVDTGMSRYGLMPSELVDFLQELNNFPGLQIE
                     GLFTHFATADWADTNFTLQQLQVFNDVLRAAQGSGLHIPIVHAANSAATLNLPQAHFN
                     AVRPGIALYGLQPSSEWPSPIEIRPALTLKSRVSRVRQLPAGAGVSYGRTFVTQHPTI
                     AALIPVGYGDGYPRALSNRSSVLIRGRRAPIIGRVCMDQFVVDVSNIPGVQLDDEVVL
                     IGVQGNENIQAEELAELAGTINYEITTSLLPRIPRLYIRAG"
     gene            complement(1827..2558)
                     /locus_tag="AC16V7_16485"
     CDS             complement(1827..2558)
                     /locus_tag="AC16V7_16485"
                     /inference="COORDINATES: protein motif:HMM:NF015722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HIR6258772.1"
                     /translation="MFLLLSIHSFVLNACLLTYRPAQNHTNMVKVPAGTFFMGMNQMK
                     SEDNRPEHEIWLDSFLIDIYEVTNKQYRGCVAAGVCNEPEDLRFFADEHFADHPVVYI
                     TWYDASDYCQWQKKRLPTEAEWESAARGDEGNLYPWGNRLYLDKLNANNQYLGTTPVG
                     NFPAGASPYGAFDMAGNVWEWVEDWYEAYPGSNFQSDLFGHKYKVVRGGSWNHPAEDA
                     RSTQRDMAHPERAIRVVGFRCASTR"
     gene            complement(2648..>3355)
                     /locus_tag="AC16V7_16490"
     CDS             complement(2648..>3355)
                     /locus_tag="AC16V7_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6258773.1"
                     /translation="LSVENLRVSYGGIRALHGIDFSVDEGEIVCIIGANGAGKSTTLR
                     AISRMIQPESGSKLVFKDIDLLKYPADKIVSELGITHVPEGRRLFGNLTVMENLTLAA
                     FSRKNGSGIEQDYERVFSLFPRLAERKTQKAETLSGGEQQMLAVGRALMSGRKMMLLD
                     EPSMGLAPVLMMTMFDALREINKEGTTILLVEQNARLALQFAQRGYVLENGHLVLQGN
                     SKELITNPDVKKAYLGG"
BASE COUNT          807 a          815 c          831 g          902 t
ORIGIN      
        1 ctcaggttat tcacgttgaa gactgggtga tacgccagcg ggtgccggct ggtgagggcc
       61 ctttccccac cattattatg ttgcacggtt ggactggcga cgagaatgta atgtggatct
      121 atgccacccg aatgccgaaa aatgctctct tgcttgcacc gagaggctta tactccacgc
      181 cattaggcgg gcatggatgg catccctata aagcaaaagt ctggccttta atggatgatt
      241 tccgcgcctc caatgaggct tttttagagt tgctaaacat caacaatttc cctttggtag
      301 atttcagtcg tttgcatttt gttggattca gccaaggggc agccatgtcc tatgccttcg
      361 ctctggaaaa tggtgatcgc ctgagctctg tggtgggact ctcgggattt cttccaattg
      421 gggctgatgc tgctgcaaca aacgaaagtc ttcatcacaa acctatattt atagcccacg
      481 gcaaccagga tttcctggta ccagtctacc gggcacggga aacagtcaat ttatttcaaa
      541 aagcaggcgc tgatgtgacc tactgtgagg atgatgtagg ccacaagctg agcgccaatt
      601 gtttccgtgg aatggaagcg ttttttgtga aacaggggtt ttagccgttc cggaggggga
      661 aatggtgtag ggataactaa ccagcgcgta tgtatagcct tggtatgcgc ggaagcaggc
      721 tggtagtaat ttcatagttg attgtacctg ccagctcagc taattcctct gcctgaatat
      781 tttcattacc ctgaacccca attaaaacca cctcgtcatc aagttgtacg ccagggatgt
      841 tgctgacatc aacaacgaat tggtccatgc aaacccgacc tatgatgggt gcgcggcgac
      901 ctcgtataag cacactgctt ctgtttgaga gggcgcgagg gtagccatcc ccgtaaccga
      961 caggtattaa tgcagcgatt gtggggtgtt gtgtgacaaa agtgcgacca tagctaactc
     1021 ctgccccggc gggtagctgg cgtacccggc taacccggct ctttagggtt aaggctgggc
     1081 gaatctcaat gggggacggc cactcgctgg atggttgtaa tccatagaga gcgatccctg
     1141 ggcgcacggc attgaagtga gcttgaggaa ggtttaatgt cgctgcgctg ttggcggcgt
     1201 ggacgattgg aatgtgtaat ccgcttcctt gagcggcgcg taagacatca ttgaagacct
     1261 gcagctgttg cagtgtgaaa tttgtgtccg cccaatcggc ggtggcaaag tgagtgaaaa
     1321 gaccttcgat ttggagacct ggaaaattgt tcagttcttg taagaaatcc actagctctg
     1381 aaggcattaa tccataacgg ctcatgcctg tatcgacctt gatgtggaca gggattgatg
     1441 cccctaggga gatagcttga gccgagagtg ccagggcaga ttctgaggtt gtcaggctgg
     1501 gagtgagctt ccagcgggcg attaactcag caccgtctgg cggggtgtaa cccatgatca
     1561 aaattggggc tgttatgccc gcctggcgca attcgatccc ttcgatggcg cgatgaactg
     1621 ctaatctggt tgcccccgct tctaacgtgg tacgagcaac agctaccgcg ccatgaccat
     1681 atgcatttgc cttgacaaca gcgatgatct cgaccgctcg acccacatgc ctttgcagcg
     1741 ctttcacatt aaaggtgatg gcgttcagat cgatttcagc ccaactaagt atgccttgat
     1801 tcaacgatat aattttctgc tcctatttag cgcgttgatg cacaccggaa accaaccaca
     1861 cggattgcgc gttcaggatg agccatatcg cgctgtgtgg aacgtgcatc ttctgcagga
     1921 tggttccagg atcctccccg gacaaccttg tatttatgac cgaaaaggtc actctggaag
     1981 ttgcttccag ggtaagcttc ataccagtct tctacccact cccatacatt gcccgccata
     2041 tcgaatgcgc cataagggct ggcacctgca gggaaattac ccactggggt tgtgcccaaa
     2101 tattgattgt tggcattgag tttatccaga tagagtctat ttccccaagg atataaattg
     2161 ccctcatctc cgcgagctgc gctctcccat tccgcttcag ttggtaagcg ctttttttgc
     2221 cactggcagt agtcactggc atcataccat gttatatata caaccggatg atcggcaaag
     2281 tgctcatcag caaaaaagcg taaatcctca ggctcattac atacccctgc agcgacacaa
     2341 cctcgatact gtttattggt tacttcgtaa atatctatca agaatgagtc tagccagatt
     2401 tcatgctctg gcctgttatc ttctgatttc atctgattca tgcccatgaa aaatgtcccg
     2461 gcagggactt tgaccatatt tgtgtgattt tgtgccgggc ggtatgtgag cagacatgcg
     2521 ttcaatacga aagaatgtat tgagagtaat agaaacaagg ttacacgagg tgtcgctatc
     2581 tttttgtacg cctcaggatt gaataagtcg cttattaaga acaatattta gggaagtctt
     2641 ggttggttca gcctcccaga tatgctttct tcacatctgg gtttgttatg agctcttttg
     2701 agttgccctg gagcactaaa tgaccatttt ccaagacata tccacgttga gcaaattgaa
     2761 gtgctagacg agcgttttgc tcgacaagga gaatggttgt accttcctta tttatttctc
     2821 gcaaggcatc aaacatggtc atcatcagga cgggagctag tcccatagaa ggctcgtcca
     2881 gcagcatcat cttcctgccg ctcatcaatg ctcgcccgac tgctaacatt tgttgttcgc
     2941 caccgctcaa tgtctcagcc ttttgtgttt tccgctctgc tagacgggga aagagcgaaa
     3001 acacgcgctc ataatcttgt tcaataccag acccgttttt acgcgaaaag gctgcaaggg
     3061 ttaaattctc catgaccgtc agattgccaa atagtcgcct gccttcgggg acatgggtta
     3121 tccccaattc gctgactatc ttatcggcag ggtatttgag caaatcaatg tctttgaaaa
     3181 ccagcttact gcctgattct ggctgtatca tgcgggagat tgcccgtagg gttgtgctct
     3241 tgccagcccc attggcacca ataatacaaa caatttcccc ttcatcaacg ctaaaatcta
     3301 tcccatgaag ggctcgtata ccaccatatg agacacgtaa attttctacc gagag
//
LOCUS       DBNHOH010000914         2228 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_956805, whole genome shotgun sequence.
ACCESSION   DBNHOH010000914 DBNHOH010000000
VERSION     DBNHOH010000914.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_956805"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="AC16V7_16495"
     CDS             <1..330
                     /locus_tag="AC16V7_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR6258774.1"
                     /translation="SPAVEPPLIVSATTRVSWFVYVVRLSPNIDRDKVALDLGERGVP
                     ARPYFLPIHLQPYMVERFGYRPGDYPVTEDLGRRSLALPFSGVMSEAQVDYVCQTLHQ
                     VLQTGVD"
     gene            551..1648
                     /locus_tag="AC16V7_16500"
     CDS             551..1648
                     /locus_tag="AC16V7_16500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258775.1"
                     /translation="MKAKILTLIMLFAALALSACAQAGIIMPVVNASASSIERTEVKC
                     IRDTEDTRLLIQGTQGYCLQYPAEYDLALPSESEIMLIKRSMVNVTEPRAHILVQPAA
                     GKTVEQVADQLVADYSVPGLEITRTALTIDGEQAVVLDGLSGQDVNRQLVVIHEGTLY
                     HLSFLPMDKSQVETYSQAQALFDTVIRTFNFRPESNVCPDCPPSTSADEGQAEIDPQS
                     ASISGWVWHDLCDSGKDGQPPPAISTLVGCVGEETALGPYHANGVQDLVDPSIEGVVV
                     SLGEGECPSTGLAEMSTIVTDLSYYVTGLSAGTYCVSIDPQREPNFSILRPGIWTYPY
                     ISQDIISTTVTLGPGEYRGMVNFGWDYQFKP"
     gene            complement(1731..>2228)
                     /locus_tag="AC16V7_16505"
     CDS             complement(1731..>2228)
                     /locus_tag="AC16V7_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR6258776.1"
                     /translation="TLGKVIGGGLPVGAYGGRQEIMSLIAPSGPVYQAGTLSGNPLAM
                     SAGIATLRNLGQPGVWAELESGVDRLTAGVAEAAAEAGVPIQQTRVGTMFCTFFSATP
                     VRDWPTAKLSDTARFGRFFTEMLKQGVYLAPSQFEAAFFSTAHTDEIVDQTITAASKA
                     FKLSL"
BASE COUNT          514 a          585 c          614 g          515 t
ORIGIN      
        1 agtcctgctg tggagccacc cctgattgta tctgctacca cgcgcgtcag ctggtttgtc
       61 tacgtggtgc gcctatctcc aaatattgac cgggataaag tggcgctgga cttgggagag
      121 cgcggcgtgc cggcgcgtcc ttacttcttg cctatccatc tgcaaccgta catggtcgag
      181 cgctttggct accgacctgg cgattaccct gtcaccgagg atctcgggcg ccgcagcctg
      241 gcgctgccct tttcgggggt gatgagcgaa gcacaggtag actacgtctg ccagacactg
      301 caccaggtat tacaaacagg cgtcgattag ccttagtcac aacacacatc gcttgtaatt
      361 caggggaata tcacggttcg agggcatgta tttttctacg cttgggaggc tggaaaaaaa
      421 cagaaaattg ggtaaatctt tggaacatta tggggtcatg ttgcgtctaa tgcctgatgt
      481 ctggttatga caccaaaagg ttttcaaaag atggtgttcg atgccagcga taatactcag
      541 gagactaata atgaaagcca aaatcttaac cctaataatg ctttttgctg cgctggcact
      601 ttcagcttgt gcccaggcag ggatcatcat gccggtggtt aacgcctcag cctcttcaat
      661 agagcgcaca gaagtcaaat gcatacgcga taccgaagac acgcgactgc tgatccaagg
      721 tacacagggg tactgtttgc aatacccggc agagtacgat cttgctcttc ccagcgaaag
      781 tgaaatcatg ttgatcaaac gctcgatggt caacgtcact gaaccgaggg cgcacatcct
      841 agtacagccg gcagcaggaa agacggttga gcaggttgct gaccagcttg tggcggatta
      901 ttctgtaccc gggctggaga tcacacgcac agcactgacc atcgatggtg agcaggctgt
      961 ggtgttagac ggattgagcg gacaggacgt aaaccggcag ttggtagtta tccacgaagg
     1021 caccttgtat catctgtcct ttctgccgat ggacaaaagc caggttgaga catattccca
     1081 ggcgcaggct ctgtttgaca cggttattcg cacctttaac ttccgcccgg agagcaacgt
     1141 atgtcccgat tgccctcctt caaccagtgc ggatgaaggt caagcggaga ttgatccaca
     1201 gtctgcatca attagcggat gggtgtggca cgacctgtgc gattctggta aggatggaca
     1261 gccaccgcct gctatatcta cactagttgg ttgtgttggt gaggagactg cccttggacc
     1321 ttaccacgca aatggtgtcc aggatctggt tgacccctcg atcgaaggtg tggttgtgtc
     1381 ccttggggag ggcgagtgcc cttccaccgg tctggcggaa atgtcaacga tcgtgacaga
     1441 cctgtcctat tatgttactg gtctgagcgc gggaacgtat tgtgtctcga ttgacccgca
     1501 gcgggagcca aacttctcga tcttgcgtcc cggaatatgg acgtatccat acatttcaca
     1561 agacataatc agcacaacgg tgactttagg ccctggagaa taccgaggta tggtgaattt
     1621 cggatgggac taccaattca agccatgaga gtgtctgaat actcgctgaa cataacaacc
     1681 ctcgacatta tgtcgagggc tgtctgtttt aatctggagt gatgactgaa ttacagggag
     1741 agcttaaatg ccttgctggc cgctgtgatg gtctgatcga cgatctcatc cgtatgcgct
     1801 gtggagaaga atgccgcttc aaattgcgag ggagccaggt aaacaccctg ttttagcatc
     1861 tcggtgaaga agcgcccaaa acgggctgta tcgctcagct tggctgtcgg ccaatcgcgc
     1921 acaggcgtgg cgctgaagaa tgtgcagaac atagtgccca cacgggtctg ctgaatgggg
     1981 acaccggcct cagccgctgc ttccgccacg ccagcagtca ggcggtctac gccactttcc
     2041 agctcagccc acacccctgg ctgtcccaga ttgcgcaacg tagcgatgcc tgcactcatt
     2101 gccagtggat ttccagagag cgtaccagcc tggtacactg gtccagaggg ggcgatcagc
     2161 gacatgatct cctgccgccc gccataggcg cccacaggca gcccgccgcc aatcaccttg
     2221 cccagcgt
//
LOCUS       DBNHOH010000915         2039 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1276321, whole genome shotgun sequence.
ACCESSION   DBNHOH010000915 DBNHOH010000000
VERSION     DBNHOH010000915.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1276321"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /gene="obgE"
                     /locus_tag="AC16V7_16510"
     CDS             <1..1263
                     /gene="obgE"
                     /locus_tag="AC16V7_16510"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HIR6258777.1"
                     /translation="YMFLDQAVIIVRAGKGGDGVVHFRREKYVPFGGPDGGDGGKGGD
                     VYLEVVPTLNTLSSFRHKSRFQAQDGVRGGKQNMTGRSGEDLFINVPPGTIVYDDETG
                     ETLGDLTQAEQRMLVAKGGRGGRGNARFTSSRNQAPRQAERGEPAQERQLRLELKLIA
                     DIGIVGVPNAGKSTLLASVSNAKPKIAPYPFTTLEPNLGVSELDDETTLVLADIPGLI
                     EGAHMGLGLGHDFLRHIQRTRVLIHLLDGLAEDPLLDLAQINSELALFDPDLTEKPQI
                     VALNKIDLPEVAAIWDKIETSLKERGYEPMAVSALAGTNVRQLLYRTAQLLDQVPPAS
                     EIAAMPVYRVPSDPREFHVQREPKGWRVSGEAIERAAAMTYWEYDQSVRRFQRILQTL
                     GIEDALRQAGVKTGDTVYIGEFELEWED"
     gene            1264..1881
                     /gene="nadD"
                     /locus_tag="AC16V7_16515"
     CDS             1264..1881
                     /gene="nadD"
                     /locus_tag="AC16V7_16515"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HIR6258778.1"
                     /translation="MALGIFGGTFDPPHLGHLILAQEAQSQLGLDKVLWVLTAGPPHK
                     TGQVITALQHRLDMLSAATCDTPDFEISRVDIDRSAPHYAVDTLRLIARSHPEAELIY
                     LMGGDSLHDLPTWFKPDEFVNACDAIGVMARPGEQAPLDSLERQIPGITRKVRFIEAP
                     LLEIASSDIRARVAQGRPFRYYLPPAVYQIILDRKLYIHRSYAIG"
BASE COUNT          444 a          506 c          584 g          505 t
ORIGIN      
        1 tatatgtttc tggatcaagc agtaattatc gtgcgcgctg gaaaaggggg cgatggagtg
       61 gtccatttcc gccgcgaaaa gtatgtccca tttggggggc cggacggtgg tgatggcggc
      121 aagggcggtg atgtctattt ggaagttgta cccacgctta acaccctgtc ttcttttcgt
      181 cacaaatctc gctttcaagc ccaagatggg gtgaggggcg gcaagcagaa catgactggg
      241 cgatcaggcg aagatctatt tatcaatgtc ccaccaggga ctatcgtcta tgatgatgag
      301 accggcgaga cgctgggtga tttgacgcaa gcagagcagc gcatgctagt ggctaaggga
      361 ggcaggggcg ggcgcggcaa tgctcgtttt acctcgtccc gcaaccaggc tccgcgccag
      421 gctgagcgtg gcgagcctgc ccaagaacgc cagttgcgct tggagctcaa gctgattgct
      481 gatatcggca tcgttggcgt tccgaatgct gggaaatcta cgcttctagc ttcggtctct
      541 aatgcaaagc caaagatcgc gccctatccc tttaccactc tagagcccaa tctgggtgtg
      601 tctgagctgg atgacgaaac gacgcttgtc ctggcggata tccctggttt gatcgaaggt
      661 gcgcacatgg gcttaggtct cgggcatgac ttcttgcgtc acattcaacg tacgcgtgtc
      721 ttaatccatc tgcttgatgg tctggcggaa gacccgctgc tcgatttggc gcagatcaat
      781 agcgagctgg cgctttttga tccagacctg accgaaaagc cccagattgt agccctcaat
      841 aaaattgatc tgccagaagt ggcggctata tgggacaaaa tagagacatc actgaaagag
      901 cgtggttacg agcccatggc agtttctgct cttgccggca ccaatgtgcg ccagctgctc
      961 tataggacgg ctcaactgct ggatcaggta ccccctgcga gtgagatagc tgctatgccg
     1021 gtctatcggg ttcctagcga cccgcgcgag ttccatgttc agcgcgagcc aaaaggctgg
     1081 agggtttctg gagaagcgat tgagcgagct gctgccatga cgtactggga atatgatcaa
     1141 tcggtgcgca gatttcaacg cattctgcag accttgggta tcgaggatgc tttgcgccag
     1201 gcaggtgtga aaacagggga cacagtatat atcggcgagt ttgagctgga gtgggaggat
     1261 tagttggcac tgggtatttt cggcgggaca tttgaccctc cgcaccttgg gcatttaatc
     1321 ttagcccaag aggcacagtc tcagcttggt ctggataagg tgttgtgggt tcttacagca
     1381 ggcccgccgc acaagacagg gcaggtaata acagcgctgc aacatcggct agatatgctt
     1441 tccgctgcaa cctgtgatac tcctgatttt gagatctccc gtgtggatat tgatcgctct
     1501 gcgccgcatt acgcggtgga taccctccgc ctgattgccc gatcccatcc cgaggctgaa
     1561 ttgatctact tgatgggggg tgattcactg catgacttgc cgacttggtt taaaccagac
     1621 gaattcgtga atgcctgtga tgccatcggt gtgatggcgc gacctgggga gcaagcgcct
     1681 ttggatagtt tggagaggca aatcccaggc atcacgcgta aagttcgctt tatcgaggcg
     1741 cctttgttgg agatcgcatc gtctgatatc cgcgccaggg tagcccaagg tagacctttt
     1801 cgttattatc tgccaccagc agtttaccaa atcatcttgg atcgaaaact ttatatacat
     1861 agaagttacg caattggata ggtggcagtg gggtttgggc gggcgatgcc cacccaaacc
     1921 ccacctcctt tcctcttcaa ctgcgtaagt cctaatacaa taaagggtct ttcaaatgcc
     1981 gctgctcttc gatgccctgt tttcatctgt ggcgataagc cattttgctg ttgacttgc
//
LOCUS       DBNHOH010000916         9982 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_570598, whole genome shotgun sequence.
ACCESSION   DBNHOH010000916 DBNHOH010000000
VERSION     DBNHOH010000916.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9982)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9982)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9982
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_570598"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1538
                     /locus_tag="AC16V7_16520"
     CDS             <1..1538
                     /locus_tag="AC16V7_16520"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HIR6258779.1"
                     /translation="AACSNRTHDSLFQRAIRQVGLNPYYLELVNLREQCSRVHVWQSE
                     QANRKAQELVRIAVGRASKAKPVHKQLHQARPSALVIGGGLAGMTAALAIGDSGYNVH
                     LIERTEVLGGNLQNLYYVAEGYNPRRLGRDLINRVRAHSRIAVLTRAELVRHEGHMGD
                     FRSTLRVSRPDGSSEVITIEHGVTIVATGGHETRQHPWLSLPGVITQRELEEMVIHRP
                     KEIADLKDVVMIQCVQAEGAPQYCSRVCCTNTIKNAIRLKLFNPNCRVSVLYKNIVTY
                     GFREEFYNEARRLGVIFIRYTDDSPPELKFTDKHIEVHARDLSLGRWLKLPADLITLS
                     MPIIPSEGTRELAKILRVPLSTEGFFEEAQMKLRPMDFMREGIFLAGMAHYPKFIEES
                     LTHALATAGRAVTLLSQSKALYLGGVVAEVDTSKCVGCLTCTRTCPFQIPQVIEIEGR
                     AGVGGLGGAAFIEPALCQGCGTCTGECPANAIQLVNYTDEQVMLREVGGLGQWLPLIV
                     TNN"
     gene            1607..2110
                     /locus_tag="AC16V7_16525"
     CDS             1607..2110
                     /locus_tag="AC16V7_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HIR6258780.1"
                     /translation="MSTEASTAFEPEITAFYCIYCGYMAADTAGALHIQYPANVKFVR
                     LPCTGKSDIRYFLEAFEQGADGVYVVACPLGNCHHVRGNERGHARLTRAKQILDSIGL
                     GGERLDMFFMSGSQGHSFAMAAQTMTERIRKMGPNPLKLQAEGKKIQAVTVEEEEENS
                     FRGRKKK"
     gene            2107..6708
                     /locus_tag="AC16V7_16530"
     CDS             2107..6708
                     /locus_tag="AC16V7_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6258781.1"
                     /translation="MTPVGAVMVVGGGIAGMQASLDLADQGFKVYLVETKSAIGGKMA
                     QLDKTFPTNDCAMCTISPKLVEVGRHPNIQVMTETEVLDIQGRAGDFTAYLEHHPRYV
                     DPNKCVSCGDCVKVCPVIVPDRFNEGLSPRRAIYKLYPQAVPNAFAIEKRGIAPCRDA
                     CPAGQRAQGYIALIREGRWDDALRVIKMDNPFPGICGRICNHRCETACNRGLVDEPIN
                     IRALKRFVTDKVYSEPRQSLQPAPRQHDQRVAIIGAGPCGLTAAQDLALAGFGVTVFE
                     AMPVAGGMLRLGVPEYRLPTEIIEREVQDIIDLGVDLRLNHQVNNLDEVFEQGYEAVL
                     IAVGAHEGIRLPIPGADLQGVLVNTHFLRDVRLGVYSENGKNPSARRSDLGKKVLVLG
                     GGNVAIDCARTAVRMGCEVHLTCLESREQMPAHAWEVEAAEAEGVILHPGRAFERILD
                     DGQGRAGGVECTRVSSFSFDERGRLLVEKVADSNHILDCDTVIFSVGQRAGLAFIPDD
                     ANVGMTERQTIAINPNTLATSRPGVFAAGDCISGTAFVIEAVDRGHTAAQSIIRYLQG
                     EALEPLAKPELPVVRLSQGEIAERMARGEIHPRPRVPMPELTVEQRRDNFAEVEGGYD
                     DASAQDEAARCLACGICSECMSCVFACGAKAIEHDMVAHTESVRVGAVILAPGYQVYQ
                     AELSEEYGYGRYPNVVTSLQFERLLSASGPTMGHVQRPSDGKAPRRVAFLQCVGSRDQ
                     THDYCSAVCCMYATKEAVIAKEHDPDLEVHVYMMDMRAFSKGYWGYFERAQDRYGIQY
                     HRCRVSALREDPDTHELYLQVAEETSDGLRPITERFDMVVLSVGMEISPAVRELGRRI
                     GIEMDEYGFCHTVQFNPVETSRPGMYAVGPFREPKDIPESVVEASGAAGAAAVDISAA
                     RFTLTERREIPPERDVGEESPRIGVFVCHCGSNIAGFLDVPQVTQYAASLPGVAHAEA
                     NLYTCSQDSIRHITEVVKENGLNRVVVASCTPLTHAPLFQDSIRAAGLNPYLFEMANI
                     RNQCSWVHSREWDVATGKAKDLVRMAVARAALLQPQHTIELSVQRAALVVGGGVAGMT
                     TALSLAGQGFPVHLVEKEAELGGNLRQVYTAAPSLNGGKTPEGPQEILRRLETQVRAD
                     KLITLHLSSQVASSSGFMGNFNSTIQGPDGQRQEITHGATILATGASEYRGPEYGYGS
                     HPRIVTQMELESLFDNQQATIDNLKSVVMIQCVGPGENFCSRICCTVALKNALALKEK
                     NPGLQVTILYRDIRTYGFKERLYTEARRKGVLFLRYDDQHKPQVQIGEAADQDLAVSA
                     EQPIAVKVRDAALGRDIELHPDLVVLSMPVVPNPQVHELSALFKVPADADGFFLEAHV
                     KLRPVDFSTDGIFMAGMAHYPKLLDESIIQAQAAAARAARVLSREALIAGGRVAVVDE
                     TRCTGCLTCVRICPFNVPKIQANLSGVGNILGAAYIEAAVCQGCGQCAAECPARAIQL
                     MHYTDAQMAAKVRALVELDAGFVPLEEITIGSTRPGSDN"
     gene            6767..7183
                     /locus_tag="AC16V7_16535"
     CDS             6767..7183
                     /locus_tag="AC16V7_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:6965..6967,aa:Sec)
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HIR6258782.1"
                     /translation="MGDTSLQIIAYCCEHCAYAAADLAGGLRMQYPPEVKVVQLPCTG
                     KLDVLMVLQAIEDGADGVLVAGULPGDCHYLEGNVNARRRVERIKELLTQIGLEPERV
                     RMENMSAAMAGKFVSVAKEMTEQIATLGPNPLKNAD"
     gene            7267..7833
                     /locus_tag="AC16V7_16540"
     CDS             7267..7833
                     /locus_tag="AC16V7_16540"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6258783.1"
                     /translation="MLPEQIKTYREIVTLRDGANVLLRPMTLEDEKGMIELFTPVSDE
                     DLRYLRDNVRDPSVIREWCDKLDYAKVLPLLAVVKERIVGEATLHFRKGPRRHIGEVR
                     IYLAKDFRQRGLGTKMLKTLIELARKQDLHILLAEIVTEQTQVIKAFQNLGFVLRCTF
                     DDFFMLPDGDLRDAAILTLALRPKTYDF"
     gene            7852..8535
                     /locus_tag="AC16V7_16545"
     CDS             7852..8535
                     /locus_tag="AC16V7_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HIR6258784.1"
                     /translation="MIVAEQKSLEELKTLVSGAEKVLVVGCGTCVTVCFAGGAREAAI
                     AASSLRMTSKLEGNPLEVTDVTVQRQCEWEYLDQIAEQVKDADIVLSLGCGVGVQAIA
                     EHFDQTWVVPGLNTKFLGLPTEQGVWAERCAACGDCVLGLTGGICPIARCSKSLLNGP
                     CGGSEAGHCEINPEIPCAWQLIYDRLNSLGKLNVLLEFQPPKNWRTSRDGGPRKIVRE
                     DLRLVMERE"
     gene            8595..9626
                     /locus_tag="AC16V7_16550"
     CDS             8595..9626
                     /locus_tag="AC16V7_16550"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - L-methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HIR6258785.1"
                     /translation="MTADEKSNGYLVGSNLERVLRAGHFAVTGELGPPQSADGEVIRK
                     KAALLRGYVDAVNITDNQTAIVRMSSIGAGAILVQEGLEPVIQMTCRDRNRLAIQSDI
                     LGAYALGMRNLLCLSGDHQTFGNHPTAKNVFDIDSIQLLHLVTAMRDEKIFECGDAFK
                     GQEPRFFTGAAAAPFADPIDFRPYRLAKKVNAGANFIQTQLVYDVPAFARYMEKVRSL
                     GVHKQTYILAGVGPLKSPGMARYMKKDVPGILVPDEVIERMEGAGAPWTGKKADELSK
                     EDKKERSTAWKNEGIQICIELIQALRQIEGVAGVHIMAIEWEEAVKPIVEGAGLYPRP
                     VVEVEAAPL"
     gene            9686..>9982
                     /locus_tag="AC16V7_16555"
     CDS             9686..>9982
                     /locus_tag="AC16V7_16555"
                     /inference="COORDINATES: protein motif:HMM:NF024581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR6258786.1"
                     /translation="MSEPSTQTVTINTDLARRIQDELGQNVYLCYQCVKCTSGCPVGE
                     FFDWQPNQVMRALQLGQEDIALESQTPWLCASCQTCTTRCPQGLDITAIMEFLTR"
BASE COUNT         2217 a         2745 c         3035 g         1985 t
ORIGIN      
        1 tggccgcatg tagcaaccgc acacacgact cgctcttcca gcgcgccatt cgccaggttg
       61 gcctgaatcc ctactatttg gaattggtga acttgcgtga acaatgcagc cgggtgcacg
      121 tctggcagtc cgagcaggcc aaccgtaagg cacaggagct ggtacgcatt gccgttgggc
      181 gcgcttcaaa agcgaagcca gtgcacaagc aactgcacca agcccggccc tccgccctgg
      241 taatcggggg aggcctggca ggcatgaccg cagccctcgc tatcggcgac agcggttata
      301 acgtgcacct gattgaacgc accgaggtct tgggcggcaa cctacagaac ttgtattacg
      361 tcgccgaagg ctataatcca cgtcgcttgg ggcgcgatct gatcaaccgc gtgcgcgccc
      421 actcgcgcat tgctgtctta acccgggcag agctggtgcg tcacgagggg catatgggtg
      481 acttccgctc aacgctgcgt gtctcgcgtc cagatggctc ttctgaggtg attaccatcg
      541 agcatggcgt gaccatagtc gccaccggcg gtcatgagac acgccagcat ccctggctaa
      601 gcctgccagg ggtgatcacc cagcgcgaac tcgaggagat ggtcatccat agacccaagg
      661 agatcgccga tctaaaagat gtggtgatga tccagtgcgt ccaggcagaa ggagcccccc
      721 agtactgctc gagagtgtgc tgcaccaaca ccatcaagaa cgccatccgg ctgaagttat
      781 ttaatccaaa ctgccgggtg agcgtgttgt ataagaacat cgtcacttat ggcttccgcg
      841 aagagttcta caatgaagcg cgccgtttag gtgtgatttt tatccgctac acagacgact
      901 ccccccctga acttaaattc acggacaagc atatcgaagt gcacgcccgt gacctctcct
      961 tgggtcgttg gttgaagctc ccagccgatc tgattacgct gagcatgccg atcatcccat
     1021 cggaagggac aagggagctg gcaaaaatcc tgcgcgtacc attgtccacc gagggcttct
     1081 tcgaagaggc acagatgaaa ttgcgcccga tggacttcat gcgtgagggg attttcctgg
     1141 ctggcatggc acactacccc aagttcattg aagaaagcct cacccacgcc ctggcaacgg
     1201 ctggtcgcgc cgtgaccttg ctttcacaga gcaaggcgct ttacctggga ggcgtggtgg
     1261 cggaagtgga tacatccaaa tgcgtgggct gcttgacctg cacgcgcacc tgcccattcc
     1321 aaatccccca ggtgatcgaa atagaaggac gagcgggcgt gggcggcctg ggaggcgcag
     1381 ccttcatcga accggcactg tgccagggct gcggtacatg cactggagag tgtccagcca
     1441 acgcaattca actggtcaac tacactgatg aacaggtcat gctgagagag gtcggcggat
     1501 tggggcagtg gctgccactg atagtgacaa acaactaaaa catatctcaa aacaaggtgc
     1561 gtgagtgttt atgagatgaa ctctaggata gttgaggttt gatttgatga gcactgaagc
     1621 cagtacggcg tttgaacccg agatcactgc cttctactgc atttactgcg gttatatggc
     1681 agcagataca gcgggtgcgc tgcacatcca atatccggct aacgtcaagt ttgtgcgcct
     1741 gccctgtaca ggcaagtcgg atattcgcta tttcctggag gcttttgagc agggcgccga
     1801 tggggtatat gtggtagcct gcccgctagg taactgccac cacgtgcgcg gcaatgagcg
     1861 agggcatgcg cgcttgacgc gagccaaaca gatactggat tcgattggcc tgggtggtga
     1921 acgcctggat atgtttttca tgtcgggcag ccagggacat tccttcgcta tggcagccca
     1981 gaccatgacc gagcgcatcc gaaagatggg tccaaatccc ttgaaattgc aggctgaagg
     2041 taaaaagata caagctgtaa cggtagaaga ggaagaagag aacagcttcc gtgggaggaa
     2101 gaaaaaatga cacctgttgg tgcggtaatg gtagtgggcg gcggaattgc cggtatgcaa
     2161 gcgtctttgg atttggccga tcagggcttc aaagtttatc tggtagagac taaatcagct
     2221 attggtggga agatggccca gcttgacaag accttcccca ccaatgactg tgccatgtgt
     2281 accatctcac ccaagctggt ggaggtgggc cgccatccta acatccaggt gatgactgaa
     2341 accgaagtgc tggacattca gggacgggca ggtgacttca ccgcgtacct ggaacaccac
     2401 ccgcgctatg tagaccccaa caaatgtgtc tcctgtggcg actgcgtcaa ggtatgcccg
     2461 gtgatcgtac ccgatcgctt taatgaaggg ctgtcgccgc ggcgggcgat ctacaaactc
     2521 tacccccagg cggtgcccaa tgccttcgcc attgaaaaac gcggtattgc accttgccgg
     2581 gacgcctgcc cggcaggtca gcgcgcccag ggttatattg cactgatccg tgaaggtcgc
     2641 tgggacgacg cactgcgggt cattaaaatg gacaatccct tcccgggtat ctgcggtcgt
     2701 atctgcaatc accgctgtga gacagcctgc aaccgcggcc tggttgacga accgattaat
     2761 atccgcgccc tgaagcgctt cgtgaccgat aaagtctatt ccgagccacg ccagtcactt
     2821 cagcccgccc cgcgccaaca cgatcagcgc gtggccatta ttggggctgg tccgtgcgga
     2881 ctgaccgcag ctcaagacct ggctctggcg ggcttcgggg tcaccgtgtt cgaggccatg
     2941 ccggttgctg gcggcatgtt gcgcctgggc gtgcccgaat accgcctgcc taccgagatc
     3001 atcgagcgtg aggtgcagga tatcatcgat ttgggcgttg acttgcgctt gaaccaccag
     3061 gtcaacaacc tggatgaagt cttcgagcag ggttacgagg cagtcttgat cgccgtaggg
     3121 gcgcacgagg gcatccgcct gcctatcccc ggggccgatc tgcagggcgt gctggtcaac
     3181 acgcacttcc tcagggatgt caggctgggg gtctattcag aaaacggaaa gaacccatct
     3241 gctcgtcgtt ctgatttagg aaaaaaggtg ttggtgttgg gcggtggcaa cgtagccatc
     3301 gactgcgccc gcaccgccgt gcgcatgggt tgcgaggtgc acctgacctg cctggagagt
     3361 cgcgaacaaa tgcccgccca cgcctgggag gtggaagctg ccgaggcgga gggtgtcatc
     3421 ctgcacccgg ggcgcgcctt cgagcgcatt ctggacgacg ggcaaggtcg cgctggtggc
     3481 gtggagtgca cgcgggtctc atcgtttagc ttcgacgagc gcggccgcct gctggtggag
     3541 aaggtggcag atagcaatca tatcctcgac tgcgatacgg tgatcttctc cgtagggcag
     3601 cgcgcaggct tggcgtttat ccccgacgat gccaatgtgg gcatgaccga gcggcaaacc
     3661 atcgccatca atcccaatac attagcaacc tcccgcccgg gtgtgttcgc cgcaggcgac
     3721 tgcatctcgg gcacagcctt cgtgatcgag gcggtagacc gcggtcacac tgcagcccaa
     3781 tcgatcatcc gctatctgca aggcgaagcg ctggagccgc ttgctaaacc ggagctgccg
     3841 gttgtgcgcc tctctcaggg agagattgcc gagcggatgg cgcggggaga gatccatccc
     3901 cggccgcggg tgcccatgcc cgaactgact gtagagcagc gccgagataa cttcgccgag
     3961 gtagaaggcg ggtacgatga cgccagcgcc caggatgaag cagcccgctg cctggcatgc
     4021 ggcatctgtt cggagtgcat gagctgcgtg ttcgcctgtg gagccaaggc catcgaacac
     4081 gacatggtcg cccatacaga gtcagtgcgg gtgggggcgg tcatcctggc cccgggctac
     4141 caggtgtatc aagccgagct atctgaagag tacggttatg ggcgctatcc caatgtggtc
     4201 acctccctgc agttcgagcg cctgctctcg gcctcagggc caaccatggg gcatgtacaa
     4261 cgtccttccg atggcaaagc gcctcgacgc gtcgccttcc tgcaatgcgt gggcagccgg
     4321 gaccagactc acgactactg ttcggcggtc tgctgcatgt atgccaccaa agaggcggta
     4381 attgccaagg agcacgaccc cgatttggaa gtccatgttt atatgatgga catgcgcgcc
     4441 ttctcgaagg gctattgggg atatttcgaa cgggcacagg atcgctacgg catccaatac
     4501 catcgctgcc gggtcagcgc gctgcgcgag gaccccgata cccacgagct gtatttgcaa
     4561 gtcgcagagg aaacaagcga cggtttgcgc cccatcactg agcgctttga catggtagtg
     4621 ctatcggtgg gcatggagat ttctcccgca gtgcgcgaat tggggcggcg catcggcatt
     4681 gaaatggatg aatacgggtt ctgccacaca gtacagttca acccggtgga gaccagccgg
     4741 ccaggcatgt atgccgttgg tcccttccgc gagcccaagg atatccccga atcggtggtg
     4801 gaggccagcg gggcagcagg agcggcagcg gtggatatca gcgcggcgcg cttcactttg
     4861 acggagcgcc gcgaaatccc acctgagcga gatgtgggtg aggagtcacc tcgcattggc
     4921 gtatttgtct gccactgcgg ttccaacatc gccggtttcc tggacgtgcc ccaagtaacc
     4981 caatatgccg cttcgttacc cggtgtagcg catgccgagg ccaacctgta cacctgctcg
     5041 caagatagca tccggcacat caccgaggtg gtcaaagaaa acgggctgaa tcgtgtggtc
     5101 gtagcgagct gtacgccgct gacccatgca ccgctcttcc aggacagcat ccgcgcggcg
     5161 gggctgaacc catatttatt tgagatggct aacatccgca accagtgctc ttgggtgcac
     5221 tcgcgtgaat gggatgtggc taccggcaaa gccaaagacc tggtgcgtat ggcagtggcg
     5281 cgagccgcat tactgcagcc gcagcatacc attgagttgt ccgtgcagcg ggcagcactg
     5341 gtggtcggcg gcggtgtggc aggtatgacc actgcgttat ccctggcagg tcagggcttc
     5401 ccggtgcatc tggtggaaaa ggaagccgag ctaggcggca acttgcggca ggtatatacc
     5461 gcagcgccat cgttaaacgg cgggaaaacg cccgaaggtc cgcaagagat cctgcgccgc
     5521 ctggaaacgc aggtacgggc agacaagctg atcacgctgc acttaagcag ccaagtggct
     5581 tccagcagcg ggttcatggg caacttcaac agtaccattc agggaccgga tgggcagcgc
     5641 caggagatca cccatggcgc caccatcctg gcaaccgggg cgagcgagta ccgcggccca
     5701 gaatatggat atggaagcca tccgcgcatc gtaacgcaga tggaactgga gtcattattc
     5761 gacaatcagc aagccaccat tgacaacctg aaatccgtcg tgatgatcca atgcgtggga
     5821 ccaggggaga atttctgcag ccggatttgc tgcacagtgg ccctcaagaa cgccctggcg
     5881 ctcaaggaga aaaacccggg cttgcaggtg accatcctgt accgcgatat tcgcacctat
     5941 ggattcaagg aacggctata caccgaggcg cggcgaaaag gcgtgctttt cttgcgctac
     6001 gatgatcagc acaagccaca ggttcaaatt ggcgaggcag ccgatcaaga cttagcagtc
     6061 agcgccgaac aacccatagc ggtcaaagtt cgagatgcag cgctgggacg tgacatcgag
     6121 ctccacccag acctggtcgt gctctccatg ccggttgtgc ccaacccgca ggtgcacgag
     6181 ctctcggcgc tcttcaaggt gccagccgat gccgatggct tcttcttgga agcgcatgtc
     6241 aagctacgcc cggtggactt ttccaccgac ggcatcttta tggctggcat ggcgcactat
     6301 cccaagctgc tcgatgagag catcatccaa gcccaggctg ctgccgcgcg tgccgcgcgc
     6361 gtgcttagcc gcgaggcatt gatcgctgga gggcgtgtgg cagtggtgga cgagacgcgc
     6421 tgtacgggct gcctgacctg cgtgcgcatc tgcccattca atgtgccaaa gatccaggcc
     6481 aacctgagcg gcgtagggaa catactgggc gcagcatata tcgaagctgc agtgtgccag
     6541 ggttgcgggc agtgcgccgc ggaatgtccg gcgcgggcca ttcaactgat gcattacaca
     6601 gacgcccaga tggcagccaa ggtgagagcc ctggttgaac tggacgccgg ttttgtgccc
     6661 ttagaggaga taaccattgg gagcacaaga ccgggatcgg acaattagga attggtatcc
     6721 gggttattag tcatttaccg gttattttca ggagaaggac tctcggatgg gagatacatc
     6781 gttgcaaatc atcgcatact gctgtgaaca ttgcgcttac gccgccgctg acctagcggg
     6841 tggcttgcgc atgcagtacc cgccagaggt gaaagtggtt cagctgccct gcactggcaa
     6901 actggacgtg ctgatggtgc tccaggcaat agaggatggc gctgatggcg tgctggtagc
     6961 cggctgactg cccggcgact gccattacct ggaaggtaat gtaaacgccc ggcgccgggt
     7021 agagcgcata aaggaactgc tgacccagat cggcctagaa ccggaacgcg tgcgcatgga
     7081 gaacatgtcg gcagccatgg ccggtaagtt tgtcagcgtg gcaaaagaga tgacggagca
     7141 aattgcgacg ctggggccaa atccgttaaa aaatgcagat tgagaattgt cgattaatga
     7201 ttgaacggtg actccctaag accttgttca tcaatccggg gtctcaatct gagatcagga
     7261 ggaagaatgc tgccggaaca aattaaaacc tatcgcgaga ttgtcaccct gagggatggt
     7321 gccaacgttc tgctgcgccc gatgaccttg gaggacgaga aaggcatgat cgagctgttc
     7381 accccggtca gcgatgaaga cctgcgctac ctgcgcgata acgtgcgcga cccgagcgtc
     7441 atccgggaat ggtgcgataa attggattat gccaaggtgt tgccgctgct ggctgttgtc
     7501 aaagaacgca tcgtcggaga ggcaacgcta cactttcgca agggaccacg aaggcatatt
     7561 ggcgaagttc gtatctatct cgccaaagat ttccgccagc gcggcctggg cacaaaaatg
     7621 ctcaaaactc tcatcgagct ggcacgcaag caggacctgc acattttgct agcagaaatc
     7681 gtgaccgagc aaacccaggt gatcaaagcc tttcagaatt taggcttcgt cctgcgctgt
     7741 acttttgacg actttttcat gctaccggat ggcgatttgc gcgacgcggc catcctgacc
     7801 ctggctctgc ggccaaaaac gtacgatttt taatgattgg agggtagatc tatgatcgtt
     7861 gccgaacaaa aatctctgga agaactaaaa accctggtga gtggtgccga aaaagtgctg
     7921 gtggtaggct gtggcacgtg cgtaaccgtg tgctttgctg gtggggcgcg tgaagcagct
     7981 attgcagctt catccttgcg catgacaagc aagctggaag gcaatcccct ggaggtcacc
     8041 gatgtgactg ttcagcgcca gtgcgaatgg gaatatctag accagatcgc cgagcaggta
     8101 aaggatgccg acattgtgct ctccttgggc tgcggggtgg gcgtgcaggc tatcgccgag
     8161 catttcgacc aaacctgggt agtgcccgga ttgaacacca agttcttggg cctgcccacc
     8221 gagcaaggcg tgtgggctga gcgttgtgcg gcctgcggcg actgtgttct tggcttgaca
     8281 ggcggcattt gccctatcgc ccgctgttcg aaatcgctgc tcaatggacc ctgtggaggc
     8341 tcggaagccg ggcactgcga aatcaatcca gagatccctt gtgcttggca attaatctac
     8401 gaccggttga acagcctggg caagctgaac gtgctgctgg aattccagcc gcctaaaaat
     8461 tggcgcactt cccgggatgg cggaccgcgc aagattgtgc gcgaagactt gcgcctggta
     8521 atggaaaggg agtagaacgt gatgtgtgac ttgtggcaga tcacttctta tgtatcccaa
     8581 caggaggatg agcgatgaca gccgatgaga aaagcaatgg atacctggtc ggatctaacc
     8641 tcgagcgggt gctgcgggca gggcatttcg ctgtcacggg agagttggga cccccacaga
     8701 gcgccgatgg cgaggtgatc cgcaaaaagg cggcgctgct gcgcggatac gtcgacgcag
     8761 tcaacatcac cgataatcag actgccattg tgcgcatgtc cagcattggc gcgggtgcca
     8821 tcctggtgca agaaggattg gagcctgtta ttcagatgac ctgccgcgac cggaatcgcc
     8881 tggcaattca aagcgacata ctgggtgcct acgccctggg gatgcgcaac ttgctctgcc
     8941 tgagcggcga ccaccagacc ttcggcaacc atcccaccgc caagaatgtc ttcgacatcg
     9001 attcaatcca gttgttgcat cttgtgaccg ccatgcgcga cgagaagatt tttgagtgcg
     9061 gcgacgcctt caaaggccag gagccgcgct tcttcaccgg cgctgccgcg gcgcccttcg
     9121 ccgacccgat agatttccgt ccctaccggc tggcaaaaaa ggtcaatgca ggcgcgaatt
     9181 tcatccagac tcaactggtg tacgatgtgc cagcctttgc ccgctatatg gagaaagtgc
     9241 gctccttggg ggtgcataag cagacctata tcctggcagg agtaggacca ctgaagagcc
     9301 cggggatggc gcgctatatg aaaaaagatg tacccggcat ccttgtgccc gacgaggtca
     9361 tcgagcgcat ggagggggca ggggctccct ggacgggcaa gaaagccgac gaactgagca
     9421 aggaagacaa gaaagagcgc tctacagcct ggaagaatga gggcattcag atctgtattg
     9481 agctgatcca ggcgctgcgt caaattgagg gtgtggccgg ggtgcacatc atggccattg
     9541 agtgggaaga ggcggtcaag cctatcgtgg agggggcagg attgtacccg cgccctgtag
     9601 ttgaagtaga ggctgcaccg ctatgagcaa gaagtatcgg caatccaggg gcggctcctc
     9661 aaaacggggc caaaaggaat agcctatgag tgaaccatca acacagaccg taaccatcaa
     9721 taccgatctg gcgcggcgca tccaagatga gttggggcag aacgtgtatc tgtgctacca
     9781 gtgtgtcaaa tgtaccagcg gctgcccggt gggagagttc ttcgactggc agcctaacca
     9841 ggtcatgcgc gccctgcaat tgggtcagga ggacatcgcc ctagagtcac aaactccctg
     9901 gttatgtgcc tcgtgccaga catgcactac gcgctgccct caagggctgg atatcaccgc
     9961 catcatggaa tttctgaccc gc
//
LOCUS       DBNHOH010000917         2328 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1021043, whole genome shotgun sequence.
ACCESSION   DBNHOH010000917 DBNHOH010000000
VERSION     DBNHOH010000917.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1021043"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(362..847)
                     /locus_tag="AC16V7_16560"
     CDS             complement(362..847)
                     /locus_tag="AC16V7_16560"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF015999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HIR6258787.1"
                     /translation="MDPSDHLSTPVTRYLDSIQVPYRVFTHPGPIKSLEQAAQERNQA
                     PEQVVRSIVFRLSDSKFVMVLVAGAQQISWPALREYLGVSRMSMAKPEEILSQTNYPI
                     GAVSPFGLSRPMRILADESVFQPDEVSIGSGVRYTTVILRTDDLRRALGEVETGIFTE
                     A"
     gene            complement(931..2103)
                     /locus_tag="AC16V7_16565"
     CDS             complement(931..2103)
                     /locus_tag="AC16V7_16565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR6258788.1"
                     /translation="MKNVSVIIPTYWTWPQSASHRPESASYDHPTPLDGESTLPRLLD
                     DLCEQRMQDFRVLILTGVSHPGLGQDAADHMRTLLQPYEDRLDLYVCDAPTWARLKAV
                     LDSASLSTDGLHLGSYSGIRNLQLLVPHIVGAEVIIALDDDESIKPNYLDQALTDIGN
                     EVHGQRVLGLAGPYLHPDGSVLLPERPPTGNPVRDKGLYINQAMRFLTRAQGELVPSP
                     MALGGNMVFHRDLFTRVPFDPGITRGEDIDYLINARLEGISWWFDPKLTILHLPPHHY
                     DTPPYQRMREDVFRFIYEREKLHLHGFHQPDWLEPYPGALLGDQLREQALSALEAEAT
                     VELVARYGDGQTIVKQGEDHASHYAPLYTGFAQSWRQVMAQIEADDALRRDLIGCF"
BASE COUNT          598 a          602 c          574 g          554 t
ORIGIN      
        1 tgcccacccc ctccaccgcc tcgcatcagg acaggcaagt aaatcgggtt cgggggaccg
       61 tcagggccag ccaaggcccc agagacagaa aataacaagg aacaaatgat gaggagccca
      121 aacaacttct tcatatcatt actcctttcg accttccggt gtggcacaga ttgagatttt
      181 tttacagcaa acagtaagga gatctatttt cgattttacc tattatctat ttcccattaa
      241 atatagcaaa acatattatt aatgtcaaat taaatttttt gtaattttgt gatcagtcga
      301 aaattttata agcgtgtcat ggcaagtgag cgtgagacag cagaggaatc ccatataaaa
      361 atcaagcctc agtgaaaata ccagtttcga cctccccgag ggcgcgcctc aaatcgtctg
      421 tccgtaggat cactgttgta tagcgtaccc ccgaaccgat ggatacttca tctggctgga
      481 atacgctttc atccgccagt atgcgcatcg gtctggacaa cccaaatggc gataccgccc
      541 cgatggggta gttagtctgc gacaatattt cttccggctt agccatgctc atgcgcgaaa
      601 cgccaagata ttcccgtaaa gcaggccatg agatctgctg cgcacctgcc accaagacca
      661 tgacaaactt ggaatcgctc aagcgaaata cgatactccg caccacctgc tctggagcct
      721 ggtttcgctc ttgggctgcc tgctcgagtg atttgatcgg cccgggatgg gtaaatacgc
      781 gataagggac ctgtatcgaa tcaaggtatc gcgtgacggg tgttgaaaga tggtctgaag
      841 gatccataac tacctgtcaa aatagattta gaagttacac agttggatac gtggcagggg
      901 gggttgggcg gattgccttt cgcattgtct tcaaaagcaa ccaatcagat cgcggcgcaa
      961 tgcatcatca gcttcaattt gagccatgac ctgtctccaa gattgtgcaa atcctgtgta
     1021 taaaggtgca tagtgagaag cgtgatcttc accctgcttg acgatcgtct gtccatctcc
     1081 atagcgggca accaattcca cagtcgcttc agcctcgagc gcagaaagag cctgctccct
     1141 caattgatca cccaacaggg caccggggta tggctcaagc caatccggtt ggtgaaaacc
     1201 gtgaaggtgt aacttttcgc gttcatagat gaaacgaaat acatcctcgc gcatgcgctg
     1261 gtatggaggc gtgtcataat ggtggggcgg caaatgcagg atagtcagct tggggtcgaa
     1321 ccaccagctt atgccttcca ggcgagcatt gataagatag tctatgtctt cgcctcgtgt
     1381 aatgcctggg tcgaatggca ctcgggtgaa taaatcccta tggaagacca tgtttccgcc
     1441 cagcgccatc ggacttggca caagttcacc ctgagccctg gtcaggaatc gcattgcctg
     1501 gttaatatac agccccttat ctcgcacagg gttgccagtc ggcggacgct cgggaagcaa
     1561 gacgcttcca tcgggatgca gatatggacc cgccaggcca agtacccgct gtccatggac
     1621 ctcattgcca atgtctgtga gcgcctgatc caagtaattt ggcttaatac tctcatcgtc
     1681 atccagggca ataatgacct cggcgcccac gatatgcggg acgagcaatt ggagattgcg
     1741 gatgccgcta taactcccca gatggagccc gtctgttgac aagctagccg aatctaggac
     1801 agccttcaag cgcgcccagg tgggagcatc gcacacatag agatccagac ggtcttcata
     1861 tggctgcaat aaggtgcgca tatgatcggc agcatcttga cccaatccag ggtgagatac
     1921 cccagtcagg atcaggacgc gaaagtcttg catcctctgc tcgcataggt catctagcaa
     1981 gcggggcaat gtactctcac cgtctagggg tgtgggatgg tcgtaggagg cagactccgg
     2041 gcgatggctc gcgctttgcg gccatgtcca gtaggtaggg ataataacac tgacattctt
     2101 cataatcgag ttccgtacac ccttttatac ccaagtttcc cagtaattgc aacttttcta
     2161 gcaacctgac actctaacaa aggaatagct catcggtcaa taaatcccgg ttaaagtaat
     2221 tcttgcagca gaattcactt accggaggaa ccccatgccg actaacgttc tctatcatac
     2281 ctggtttcag aatgtttgac acggattgct ggcaaggtac ccggtcct
//
LOCUS       DBNHOH010000918         2934 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1725680, whole genome shotgun sequence.
ACCESSION   DBNHOH010000918 DBNHOH010000000
VERSION     DBNHOH010000918.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1725680"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="AC16V7_16570"
     CDS             complement(<1..471)
                     /locus_tag="AC16V7_16570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR6258789.1"
                     /translation="METTLEKRTSWLDRPVVSAITLNWETILFATILILAVVSRFYDL
                     GARVMSHDETSHVYFSWLYEQGNGFSHDPVTHGPLQFHLVAFSYFLFGDSDFSARIPV
                     AIFSIATVFFMWYFRRFLGRAGAIVAALLLLISPYILYYGRYVRNEAFVGLFGVI"
     gene            complement(531..1121)
                     /locus_tag="AC16V7_16575"
     CDS             complement(531..1121)
                     /locus_tag="AC16V7_16575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258790.1"
                     /translation="MSQEEQVLLFNQQLDQLLADEMPDKVLSDLDEQTLRLAQRLSQV
                     NFSDESLNRHALYTRLRKLSSTGNSKTARHWLPSIIRITSFQVQFVTWVRATLVLLSV
                     LFFGLGIPNPSTMIETSKPLVTYNSAMASVDVAPLTQNYQIQAHHPVPIPTPASSSLP
                     DELPKVQNTGMRFTKNKPAIAHTPKPSPISLHDMER"
     gene            complement(1118..1690)
                     /locus_tag="AC16V7_16580"
     CDS             complement(1118..1690)
                     /locus_tag="AC16V7_16580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6258791.1"
                     /translation="MTTFETKIIRKAAIAEQSLNDRHAFLDRYHEYFPRVYNYVRYRC
                     GDQSITDDITSIVFEKALTKFHQYSPKRGSFGAWLFAIARNEVNAYLRSLLGRNRIVP
                     MEQVQDWPTGKLSPEEALIDGELRNELIAALEGLNERERDLLGLKFSARFTNRQIASM
                     TGLTENNVGIILYRALQKLRRQLIPLEAQK"
     gene            complement(1820..>2934)
                     /locus_tag="AC16V7_16585"
     CDS             complement(1820..>2934)
                     /locus_tag="AC16V7_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014685736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CASTOR/POLLUX-related putative ion channel"
                     /protein_id="HIR6258792.1"
                     /translation="RGYFIQANELISRITAQTCSQSGLSVVYNEILDFEGDEIYFQLE
                     ASLFGKTFGMALLAYEDSCVIGLRVRDGIIRLNPPMDTIIEPGDAIIAISADDDTVRV
                     SGLTNAPVNENAIQHPVPSLKGPEHTLILGWNHLAPLIVKELDNYVAPDSGVLIVADV
                     QNSIMESSFELENIKNQKVSFIQGDSTDRQTLDKLDIPNFQHVIVLSYSDQLDTQQAD
                     ARTLMTLLHLRDIGNQSKNRFSIVSEMLDFRNRELAEIARPDDFIVSDRLISLMLSQI
                     SENKELIHVFDDLFNPQGSEIYLKPAGDYVHLGTPTNFYTVVESARRRDQVAIGYRLN
                     DLSHDPRMTYGVMVNPDKSELVTFSEQDRIIVLSEE"
BASE COUNT          785 a          642 c          729 g          778 t
ORIGIN      
        1 aatcacgcca aataagccga caaaggcctc attgcgcaca taacgcccat agtaaagtat
       61 gtaaggggat attaacagca atagcgcagc gacgatcgca ccggcgcgcc ccagaaaacg
      121 gcggaaatac cacatgaaaa ataccgtggc gatactgaag atggctactg ggatgcgagc
      181 tgagaaatcg ctatctccaa agaggaaata tgaaaaggcc accaggtgga attggagcgg
      241 tccatgtgtt accgggtcat gagagaagcc attgccctgt tcgtatagcc acgaaaaata
      301 tacgtgcgaa gtttcgtcgt ggctcattac gcgcgcgcca aggtcgtaaa agcggctaac
      361 cactgccaga atcaggatag tcgcaaataa tatagtctcc cagttcaatg tgatagcaga
      421 aactacaggt cggtcgagcc acgaagtgcg tttttcaagg gtggtttcca tactggtcct
      481 tagaagtctt aattcccagg gctaattcaa gattatcatc tcatatgggc ttaacgctcc
      541 atatcatgta acgaaatagg gctgggtttg ggtgtgtggg ctatcgcagg cttgtttttt
      601 gtaaacctca taccagtatt ttgaactttt ggcagttcgt caggcaacga tgacgaagca
      661 ggcgtaggaa tgggaacagg gtgatgtgcc tggatttggt agttctgggt gagtggagcg
      721 acgtcgacgg atgccatcgc cgagttatat gtcaccagtg gtttggaggt ttcaatcatt
      781 gttgatgggt ttggtattcc caggccaaaa aacagtactg acagcaatac caaagtggct
      841 ctaacccagg taacaaattg cacctggaaa gaagtaattc taattatcga agggagccaa
      901 tgtctcgctg ttttagaatt gccagtagaa gatagctttc tcaaacgagt gtataaagcg
      961 tgtctgttga gactttcatc gctaaaattc acttgggata agcgttgggc aagccttaat
     1021 gtttgctcat cgagatcaga taagactttg tcaggcatct cgtctgccag gagttggtct
     1081 aattgttggt tgaagaggag cacctgttct tcctggctca tttttgcgct tccagaggga
     1141 taagctgacg ccgaagtttt tgtagggcgc gatacagaat aatacccaca ttattctcag
     1201 tgaggccagt catcgaagcg atctggcggt ttgtaaaacg ggcactgaat tttaggccta
     1261 aaagatctct ttctcgctcg ttcaagccct ccagtgcagc gatcaattcg ttgcgcaatt
     1321 cgccatcaat caacgcttcc tcaggggata gcttgccagt aggccagtct tgcacttgtt
     1381 ccatcggtac aattcggttc cgcccaagca aagatcgaag gtacgcgttg acctcatttc
     1441 gggcaatcgc aaacaaccag gctccaaagg aaccacgctt tggggagtat tggtgaaatt
     1501 tcgtcagcgc tttttcaaac acgatcgagg ttatatcgtc cgtgatgctt tgatcgccac
     1561 aacggtatcg aacatagtta tagactcgag gaaaatattc atgataacgg tctaaaaagg
     1621 cgtgcctatc gttgagcgat tgctctgcaa tggctgcctt gcgaataatc ttggtctcga
     1681 aggtggtcac aacaatgcca tgtagtcgaa tagaagttct actagttaat atgtgctttg
     1741 gcgaaaagta ttacataatg tcgccttgtt ctcgaaatta tcacacaacg agaatcaccg
     1801 attttttttg caggattgtt tactcctcag agagcacaat gatacggtct tgctctgaaa
     1861 aagtaaccaa ttccgactta tcgggattca ccatcacgcc atatgtcatt cgagggtcgt
     1921 ggctcagatc attcaggcgg tatccgatag cgacttgatc ccgtcgacgc gccgattcta
     1981 ccactgtata aaaattggtc ggcgtaccta ggtgaacgta gtctcctgcg ggcttaaggt
     2041 aaatctctga gccctgtggg ttgaataaat cgtcgaagac atggatgagc tctttgttct
     2101 cggagatttg tgagagcatc aagctaatca atcgatcgct gacaataaag tcgtcagggc
     2161 gggcaatttc agctaattca cgatttcgaa aatctaacat ctcgctgaca attgagaagc
     2221 gatttttgga ttggttaccg atatcacgca ggtgtagaag tgtcatcagg gtgcgggcat
     2281 cagcctgctg ggtatccaat tgatctgagt agctcaatac gatcacgtgc tggaaattag
     2341 gtatgtcgag cttgtcgagt gtctggcgat cggtggaatc gccctggatg aacgaaactt
     2401 tttggttctt tatgttttca agctcaaaag agctttccat gatgctattt tgcacatctg
     2461 ccacaattaa cacgccagaa tctggggcga cataattatc caattctttg acgatcaatg
     2521 gagccagatg gttccatccc aaaatgagtg tgtgttctgg cccttttagg gatggtacag
     2581 gatgctggat ggcattttca tttacagggg catttgtcag tcctgagacg cggaccgtgt
     2641 catcatccgc agagatggcg ataattgcat cacctggctc tattatcgtg tccataggag
     2701 ggttcaggcg gatgattccg tctcgaacgc gcaagccgat cacacaagaa tcttcgtaag
     2761 ccaacaaggc catcccaaat gtcttcccaa ataggctagc ctccagttga aagtaaatct
     2821 catcaccttc aaagtctaag atctcgttat agacaactga aaggccagac tggctgcatg
     2881 tttgagcggt aatacgggat atcagctcgt ttgcctggat gaaataacct cggt
//
LOCUS       DBNHOH010000919         1655 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1212725, whole genome shotgun sequence.
ACCESSION   DBNHOH010000919 DBNHOH010000000
VERSION     DBNHOH010000919.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1212725"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="AC16V7_16590"
     CDS             complement(<1..374)
                     /locus_tag="AC16V7_16590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258793.1"
                     /translation="MDIQKLWMVAEEAWSALAGRFEPLAEQMCAQHDMEGRTFGLLLS
                     VLTFEPEDTTPGHLMVRGPYTSADEFLNRLQGAADQGFLKEIPQGKYRLSENGREITL
                     NIAEEIRRVMAEVDPLPLEDSVR"
     gene            complement(505..1197)
                     /locus_tag="AC16V7_16595"
     CDS             complement(505..1197)
                     /locus_tag="AC16V7_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016679 - oxidoreductase activity, acting
                     on diphenols and related substances as donors [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HIR6258794.1"
                     /translation="MLQWAVHIGAWIPLILMGVDYFQDNLTINPIQELTSRTGKTALV
                     LLVLTLAATPANTVFGLRSALKVRRALGLYTFMYVSIHFLIFIGLDYGFNLKLLKEAI
                     FEKRYALVGFAAGLIMLPLALTSTRGWMARLGKNWKRLHRFVYLAGVLAVVHYVWLVK
                     SDIRDPLAYGLAVLLLLIARLRIVRKSLSSLRLNIMGRLRRQSKMIRIEINPVNSVER
                     IRKRIFGDLNPR"
     gene            complement(1226..>1655)
                     /gene="msrP"
                     /locus_tag="AC16V7_16600"
     CDS             complement(1226..>1655)
                     /gene="msrP"
                     /locus_tag="AC16V7_16600"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HIR6258795.1"
                     /translation="DPEQMPGQRGGWFDWPYVEGLRLDEAMHDLTILSTGLYGKELLP
                     QNGAPLRLVVPWKYGFKSIKSIVRIDLVEEMPESFWMASAPDEYGFYANVNPEVSHPR
                     WSQASERRIGELGRRDTLAFNGYADEVAHLYTGMDLRANY"
BASE COUNT          472 a          437 c          328 g          418 t
ORIGIN      
        1 cgtacagaat cttccagcgg taatggatct acttcggcca tcacccggcg aatttcttca
       61 gcgatattaa gggtgatttc ccgaccattt tcggacaatc gatattttcc ttgcggtatc
      121 tctttcaaga aaccttgatc agcagcgcct tgcaaccggt tgagaaattc atctgccgag
      181 gtatatggtc cgcgcaccat taaatgccca ggggtcgtgt cctcaggctc aaatgtcaac
      241 accgacaaga gcaatccaaa cgtacgtcct tccatgtcat gttgagcgca catttgctct
      301 gctagtggct caaatcggcc agctaatgct gaccatgcct cttcggctac catccacaac
      361 ttttggatat ccactcggct atctctccaa tacttaaatg atcatcctaa cctgaattat
      421 accggatcat acccaatgcc tgcgccacat ttaccggtct caacttaggt aagtaggtct
      481 gatgtattta atttactaga aaatttaccg aggatttaga tcaccgaata tccgtttacg
      541 aattctctca actgaattca ctggattaat ctcaatacgt atcatcttgc tttgcctacg
      601 caatctcccc atgatattta agcgcaggct gctcaaggat ttcctaacaa ttcgcaaacg
      661 tgcaataagt aaaagtaata cagctaagcc gtaggctaga ggatctcgga tatcagattt
      721 taccaaccag acgtaatgca cgactgctaa aacaccagca agatacacaa aacggtgtaa
      781 ccgcttccag ttcttaccca ggcgcgccat ccaacctcga gtggaagtca aggctaaggg
      841 caacattatc aagccagcag caaacccaac caaggcatat cgcttctcaa agatcgcttc
      901 tttcaagagt ttcagattaa atccataatc cagaccaata aaaatgagaa agtggattga
      961 tacatacatg aatgtgtaca ggccaagagc ccggcgcact tttaatgctg atctcaaacc
     1021 aaacaccgta ttagccggag ttgcagcaag ggttaatacc aatagaacca gggcggtttt
     1081 gcccgttcgg gatgtaagct cctggattgg attgatcgtc agattatctt gaaaataatc
     1141 tacgcccatc aggattaatg gaatccatgc cccaatgtgc accgcccatt ggagcaaatt
     1201 aagcctagga aatctcattg ccaaattagt agttcgcacg caaatccata cctgtgtaaa
     1261 ggtgcgcgac ttcgtcagca tagccattga aagccagcgt atcccgccgc ccgagttcgc
     1321 caatccgcct ttccgaagct tgcgaccaac gaggatgaga gacttctggg ttgacattgg
     1381 catagaatcc gtactcgtct ggtgcagaag ccatccaaaa tgactcgggc atttcttcca
     1441 ccaggtcaat cctgacaatc gattttatgc tcttgaaacc atacttccat ggaacaacta
     1501 aacgaagtgg ggcaccattt tggggcaata gctctttccc atacaagcct gttgacagga
     1561 tggttaaatc atgcattgcc tcatctagcc tcaaaccctc aacataaggc caatcaaacc
     1621 atccacccct ttgccctggc atctgctcag ggtca
//
LOCUS       DBNHOH010000920         4666 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_700404, whole genome shotgun sequence.
ACCESSION   DBNHOH010000920 DBNHOH010000000
VERSION     DBNHOH010000920.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_700404"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="AC16V7_16605"
     CDS             <1..474
                     /locus_tag="AC16V7_16605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258796.1"
                     /translation="VGGQRGNIVQLFKVDYCAPNPRPIDNFPPPRFTMSGYNKNPFIL
                     NFGWDGVVLFALNNYVRNDGFGDLYLYNSEIHKVDEKANPVNNICCYRDPHFSPDGSY
                     LLFSFQNLNEGTGSKTWLYYIPVASIGTGASYEPLPLPPIDDPRESPWPVLRPAK"
     gene            561..1430
                     /locus_tag="AC16V7_16610"
     CDS             561..1430
                     /locus_tag="AC16V7_16610"
                     /inference="COORDINATES: protein motif:HMM:NF048886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkZ-related protein"
                     /protein_id="HIR6258797.1"
                     /translation="MTSIPYELLLSYRNETYRLLPGKRISNRDEAVEFVNQRGFIYFW
                     PIRDILLPSLWTAVAGDRPVADAHDDPGHVTWGWKDSLIGKNEWYYAKVLRRKATIIS
                     MDIAPYFYALSENYGSPEDDYLILYQQGHLTQEAKAVYQAILENGPLDTIALRRAARL
                     SSRESDSRFNKALADLQAGFLLVPVAVTQAGAWRYAFAYDIVARHHPEIVEKARYIGE
                     LDARRKLVELYFRSVGGAQLRDVNILFRWKPADVSSTIDYWVENQLIRKVEIEGQSGE
                     WFILQDLLLEHRE"
     gene            1441..2457
                     /locus_tag="AC16V7_16615"
     CDS             1441..2457
                     /locus_tag="AC16V7_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258798.1"
                     /translation="MNPIPFPLLSFPSLILHADWSVTPAKRWIARATLGADGRYHAEQ
                     SQRVEHATSLLSTLGEQVGQAGAALVGFDFPIGLPLAYARRAGIKEFITILPELGKDR
                     WSKFYEPAHTPEEISIRRPFYPARAGNTRQQHLLKQLGFETINDIRRRCELSHPGRRA
                     ACPLFWTLGAQQVGKAAISGWRQVLVGENGILPNVSLWPFSGSLTELIQPGQVIVAET
                     YPAEFYTHLGVRFSNRMRGKKTGKRSQVDRAANSSCLLEFAKQAGITLTPSLEIEILD
                     GFGPAPDAEDRFDAIVGLFGMLNVVFGGRSPGEPENEDVQKIEGWILGQEWTDSNSYR
                     NKEE"
     gene            2457..3488
                     /gene="fbp"
                     /locus_tag="AC16V7_16620"
     CDS             2457..3488
                     /gene="fbp"
                     /locus_tag="AC16V7_16620"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HIR6258799.1"
                     /translation="MLSQPDNHSKIITIERHILEEQGFHPEATGVLTNILYDLALAGK
                     FIASNTARGELAKILGSTEDINIQGERVMKLDRLADETIYRLNDHTGRLAVMASEEHQ
                     HILPIPERYPTGKYVLLFDPLDGSSNVDYNASVGTIFSIYRRQSEDGPGTLEDCLQPG
                     RNLVAAGYLLYSISTMLVYTTGSGAHGFTLDPSIGEFILSHENIRIPNTPSYYSVNHG
                     YEKYWSEGVHRFTKQLQGLEGEYESLSLRYIGSLVGDFHRTLLSGGIFYYPADNKDPS
                     KPQGKLRLMYEAAPLAFIMEQAGGYASDGHRNILDIQPDTLHQRTPLFIGNRELVEEA
                     ETCIRIYDT"
     gene            3549..>4666
                     /locus_tag="AC16V7_16625"
     CDS             3549..>4666
                     /locus_tag="AC16V7_16625"
                     /inference="COORDINATES: protein motif:HMM:NF042862.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon-like protease helical domain-containing
                     protein"
                     /protein_id="HIR6258800.1"
                     /translation="MTPRKSKSTEPPQPVPPASLSSSALRKLCDPATLSFETTDELPD
                     LQDVIGQPRAIRAIELGSEVAGPGYNTFVLGLSGSGRTTLSREYLERKSANLPIPDDW
                     CYVNNFELPRQPKAIHLPAGRAVEFSDDVQELVKRCQTEISRLFDSEEYTQERDRLVD
                     DIKKKQEAEFMRLQEYVEKYNFAIVRTPFGFVLVPAVNGKPLKSEDIEKLSPEQRTKL
                     SQLQAKLSEEVDKSLDRLRELEKAASLQISDLNERTVMYLLRPLMENLQNKYQGVESV
                     LTHLEAIQKDIVEHSDQFRRSEGSPSSSNSSSQQAKQDWLGRYDVNVLVDNSDLKGAP
                     VILESYPSYTNLLGRIEHEVIMGASRTDFSMIRSGALHR"
BASE COUNT         1196 a         1241 c         1160 g         1069 t
ORIGIN      
        1 gtgggtggcc agagaggcaa cattgtccag cttttcaaag tggattattg cgcccccaat
       61 cctcgcccta tagataattt cccacctcca cgcttcacga tgtctggtta taacaaaaat
      121 ccatttattt tgaatttcgg atgggacggt gtcgtcttgt ttgcattaaa taactacgtc
      181 cgcaatgatg gcttcggaga cttgtacctc tacaatagcg agatccataa agtcgatgaa
      241 aaagccaacc ctgtaaacaa tatttgttgc taccgcgatc cgcacttcag tccggatggt
      301 agctacctgc tcttttcttt ccaaaactta aacgagggga caggcagcaa gacctggctt
      361 tattacatcc cggttgccag catcgggact ggtgcaagct acgagccgtt accgctcccg
      421 cccatcgatg acccacgcga aagtccctgg ccggtactga gaccagcaaa atagaggctt
      481 ggcagatgat aggacaaacg acgaagggag atcttgagcc ttcgtcgttt acctattgcc
      541 aatggattta taatcattgc atgacctcca tcccctatga gcttctgctc tcttaccgga
      601 atgaaaccta tcgcctattg ccaggcaagc gcatctccaa tcgggatgaa gccgtcgaat
      661 ttgtcaacca gcgcggcttc atatatttct ggcctatacg agacatactg ctccccagct
      721 tatggacagc tgttgccggc gatcgaccgg tcgcagatgc ccatgatgac cccgggcacg
      781 tcacctgggg atggaaagac tcgctgatcg gcaagaacga gtggtattac gcaaaagtct
      841 tgcgcaggaa ggccaccatc atctcaatgg acatagcgcc ctatttctac gccctatcgg
      901 agaactatgg ctcacctgaa gatgattacc tcatcctgta ccagcaaggc cacttgactc
      961 aggaagccaa agcagtttac caggctattt tagagaacgg cccactagac accatagccc
     1021 tgcgccgagc ggcacgcttg agcagccgag aaagtgactc ccgattcaat aaggcacttg
     1081 cagatctgca ggcaggcttc ttgctcgttc cggtggcggt tacgcaggct ggagcatggc
     1141 gctatgcctt cgcctatgac atcgttgccc ggcatcatcc ggaaatagta gaaaaagcgc
     1201 gctacatcgg cgagctggat gcgcgccgca agctggtcga attgtatttc cgatcagtgg
     1261 gaggagcgca gttaagagat gtaaacatac tcttcaggtg gaagcctgcc gatgttagct
     1321 ctaccatcga ttattgggtc gaaaatcaat taatccgtaa agtggaaatt gaaggccaat
     1381 caggcgagtg gttcatttta caagacctgt tgctggagca tagagagtaa gaattacttc
     1441 ttgaacccaa ttccttttcc cttgctatcc tttccttccc tcatcttgca cgcagactgg
     1501 agcgttaccc cagccaagcg ctggatagct cgcgctaccc tcggcgcgga tggtcgctac
     1561 catgccgaac agtcacaacg agttgaacat gctacttcat tgctcagcac attgggagag
     1621 caggtggggc aggctggtgc tgcgctggta ggtttcgatt tccctatcgg tctgccatta
     1681 gcttatgccc gaagagcagg cataaaggaa tttattacca tcctccctga gctgggcaaa
     1741 gatcgatggt caaaattcta tgagcctgca cacacacctg aagagatttc gattagacgg
     1801 ccattctacc cagccagggc gggaaacacc cgccagcagc acctcctgaa acaactaggc
     1861 tttgaaacaa taaatgatat acgccgacgc tgtgagcttt cacacccagg gcggcgcgct
     1921 gcctgcccgc ttttttggac attgggtgct cagcaggtgg gtaaagcagc gatctctgga
     1981 tggaggcagg tgctggttgg ggaaaatggc attttgccaa acgtatctct ctggcccttt
     2041 tccggttcgc tcacggagct tattcagcca ggtcaagtga ttgtcgcaga aacttaccct
     2101 gccgagtttt atacccacct gggggtgcgc ttttcaaacc ggatgcgtgg gaaaaagacg
     2161 ggtaaacgtt cccaggtaga tcgcgcagcg aactcttcat gtttattaga atttgccaag
     2221 caagccggga ttacccttac accttcccta gagatagaga ttctagatgg cttcggacca
     2281 gcccccgatg cggaagatcg ttttgatgct atcgtcgggc ttttcgggat gttaaatgtg
     2341 gtttttgggg gacgctcccc gggcgaacca gaaaatgagg atgttcaaaa gattgagggt
     2401 tggattctag ggcaagagtg gactgattca aacagctacc ggaataagga ggaataatgc
     2461 tatcgcaacc agacaaccac tccaaaatta tcaccatcga acgccatatt ctcgaagagc
     2521 agggattcca ccccgaggct acgggtgttt tgaccaatat tttgtatgac ctggcactgg
     2581 caggtaaatt catcgccagc aacaccgcgc gtggtgaact agcgaaaata ctgggtagca
     2641 ccgaagacat caacatccag ggtgagcgcg tgatgaagct ggaccgactg gcagacgaga
     2701 cgatctatcg actcaatgac cataccgggc gtctggcagt catggcatcc gaagaacacc
     2761 agcatatact gcccatacca gagcgctacc ccaccggaaa atacgttctg cttttcgatc
     2821 ccttagatgg ctcttccaac gtcgattaca atgcttctgt tggcacgatt ttctcgattt
     2881 atcggcgcca aagtgaggat gggcccggaa ccttggaaga ttgccttcag ccaggaagga
     2941 atctggtggc ggctgggtac ctgctttaca gtatcagcac catgctggtg tacaccactg
     3001 ggtcgggtgc gcatggcttt accctcgatc caagcattgg cgagttcatc ctcagccacg
     3061 aaaacatccg catccccaat actccctcat attacagcgt caatcatggc tatgagaaat
     3121 attggagcga aggcgttcac aggttcacca aacagctcca agggctagaa ggcgagtatg
     3181 aatctctctc attacgctat atcggctcac tagtaggtga cttccaccgc acacttcttt
     3241 ctggaggaat tttctattac ccggcggata ataaagaccc ctccaaaccg cagggaaagt
     3301 tacgcctcat gtacgaagcc gccccgctgg cgttcatcat ggagcaagca gggggatatg
     3361 cctctgacgg tcatcgcaat attctcgaca tccagccgga tacgctccac caacgtacgc
     3421 cgctttttat cgggaatcgc gagttagtgg aggaagcaga gacgtgtatc cgtatatacg
     3481 acacctgaag gcagaaattt gattgacttt aatcccgcta tctaatagac ttattaaaaa
     3541 ggctgtttat gacaccaaga aaatcaaaat ctactgagcc gccgcaacca gtccccccag
     3601 ccagcttatc atccagtgct ttgcgcaagc tttgcgaccc agctacgctg tcctttgaaa
     3661 ctaccgacga gctgccagac ttacaggatg tgatcggtca gccgcgcgcc atacgcgcca
     3721 ttgagcttgg tagcgaggtc gccggaccag gatacaacac cttcgtttta ggtctctctg
     3781 gctccggccg cacaacttta agccgcgagt atttagaacg caaatccgcc aatctgccaa
     3841 tacctgatga ctggtgctac gtaaacaatt tcgagttgcc acggcagcca aaagctatcc
     3901 acctacctgc aggtcgagct gttgaattca gtgacgatgt ccaagagttg gtcaagcgtt
     3961 gccagaccga aatctcacgt ctctttgata gcgaagaata cacccaggag cgtgaccgcc
     4021 ttgttgacga catcaaaaag aaacaagaag cagaattcat gcgcctgcaa gaatacgtgg
     4081 agaaatacaa tttcgccatc gtccgaacac catttggctt cgtgcttgtc ccagccgtga
     4141 atggcaagcc acttaaatct gaggatatcg aaaagctctc gcccgagcag cgcaccaagc
     4201 tgagccaatt gcaagccaag ctcagcgagg aggtggataa atctttggat cgcttgcgcg
     4261 agttggagaa agctgcctcc ctacagatca gcgacttgaa cgagcgcact gtgatgtacc
     4321 tgctcagacc attgatggag aacctgcaaa acaaatatca gggggtagaa agcgtcttga
     4381 cgcacctgga agccatccag aaagacatcg ttgagcattc cgaccaattc cgccggagcg
     4441 agggcagtcc ttcgtctagc aattcaagca gccagcaggc aaaacaagat tggctcgggc
     4501 gctatgatgt caacgtgctt gtggataaca gcgatcttaa aggcgccccg gtcatcctgg
     4561 aaagctaccc gtcctacacc aatctcctgg gtcgcatcga acatgaagta attatgggcg
     4621 caagccggac ggatttttcc atgatccgct ctggcgcgct gcatcg
//
LOCUS       DBNHOH010000921         2036 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_58296, whole genome shotgun sequence.
ACCESSION   DBNHOH010000921 DBNHOH010000000
VERSION     DBNHOH010000921.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_58296"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="AC16V7_16630"
     CDS             <1..865
                     /locus_tag="AC16V7_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR6258801.1"
                     /translation="NIYLKFELFQKTGSFKPRGAFNQILQLSQQERDKGAVAISGGNF
                     AQGVAYAGQVLGMRTIICMPDYTPRNYIQATQAYGAEVLLSPTFAETIELAQSYQRRG
                     FSFLHAWDNPNQMAGNGTLGLELLEDLPELTDLIISIGGGGLIAGIIVAIKALKPGVR
                     IWGVETEGADTMGQALKAGKVVQIQPTSLAKTLSAPYVAEDALLLTQKHLEKYILVTD
                     GEAFDAQLFLLERAKVITELAASCTLAAAEQISGAFEKNNHVILLLCGGNASLDNLME
                     YHRQLRGEPEP"
     gene            complement(918..>2036)
                     /locus_tag="AC16V7_16635"
     CDS             complement(918..>2036)
                     /locus_tag="AC16V7_16635"
                     /inference="COORDINATES: protein motif:HMM:NF037660.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6258802.1"
                     /translation="ALSLNNLGLVSQLLSDFVRAISLFEEALSLYRELKDKLGISNAL
                     LNLGSVAKDQGYFKQATTLLNECLVLKQEVGDRRGIGRIIAALAEIAVHQGNYARGAE
                     LAEQSLKIFRELGVKRGMIISLEVRVYAANHQGDYDLAHSLASQALTLANEIGAPRGI
                     AYSKAFLGLIAYAKGNLTEALKLLHSSLDIFRDVGDRKDVAYVLVNLARVAYRQGDNS
                     AASQMLEESLTISRELDIRWGLAFSLEIMGLLQRSEGHNDQAFSLFKESLILSAEQEN
                     LQGILNCLGALAGLAAMTGQPSLAARLFAVTDKMRDATGARMGSDDRMEYERYLSVLR
                     NQMSDAEFESTWSGGYAMTTEQAIAELEGWSINPTSHS"
BASE COUNT          532 a          547 c          491 g          466 t
ORIGIN      
        1 taatatttac cttaagttcg aactatttca aaagacagga tccttcaaac ctcgcggcgc
       61 gttcaaccag atcctacaac taagccagca ggagagggac aaaggcgctg tagccatcag
      121 cgggggcaat tttgcccagg gtgtggcata tgccgggcaa gtgctgggca tgcgcacgat
      181 aatctgcatg ccagattaca cgcccaggaa ttacatccaa gccacgcaag cctatggagc
      241 agaagtgctg ctctcgccca ccttcgccga aaccattgag ctagcacaat cctaccaacg
      301 ccgagggttt agctttctgc acgcctggga taatcccaac cagatggcag gcaacggcac
      361 gcttgggctg gagttgcttg aagacctgcc agagctcacc gacctgatca taagcattgg
      421 gggtggcggg ctgattgccg ggatcatcgt tgccattaaa gcactcaagc cgggtgtgcg
      481 catctggggt gttgagaccg agggtgctga tacgatggga caggctttaa aagcagggaa
      541 ggtcgtgcaa atccaaccga cctccttggc taaaacactg agtgctcctt atgttgcgga
      601 ggatgctctg ctactcactc agaagcatct tgaaaagtat atcctggtca cagatgggga
      661 agcttttgac gcccagctct ttttactgga gcgcgccaag gtgattaccg aactggctgc
      721 ctcatgcaca ctggcagctg ccgaacagat tagcggtgcc ttcgaaaaga ataaccacgt
      781 tatcttgctc ttatgtgggg gaaatgcctc gctggacaac ctgatggaat accaccggca
      841 attgagaggc gaacccgagc cctagaccaa taacccaggc taaggataaa ttgctatttc
      901 ctggtttggg ggtctgacta tgaatggctg gttggattga tcgaccaacc ttctaactca
      961 gcaatggctt gctccgttgt catggcatag ccccccgacc aggtagattc gaactcagca
     1021 tcgctcattt gattacgaag cacagacaga tagcgctcgt attccatccg gtcatcgctc
     1081 cccattcgag cacccgttgc atcccgcatt ttatccgtca ctgcaaacaa gcgcgccgca
     1141 agagatggtt gaccagtcat cgctgccaat cctgccaatg cccccaagca attgagaatg
     1201 ccctgcagat tctcctgttc ggctgagagg atcaaacttt ccttaaaaag gctaaaagcc
     1261 tggtcattgt gcccctcgct gcgctgtaat aaacccatga tttcaaggga aaatgctaga
     1321 ccccaacgga tatccagttc acgggaaatg gtcaggcttt cttcaagcat ttgggatgca
     1381 gctgagttat caccttggcg gtaggccact cgcgccaagt tgaccaatac ataagctaca
     1441 tcttttcgat caccgacatc acggaaaata tccagtgaac tatgtaacag tttcagcgcc
     1501 tccgtcaaat ttcccttggc gtaggcgatc aatccaagaa atgcttttga ataagcaatt
     1561 ccgcgcgggg cgccgatctc attggctaaa gtcagggctt gggaagccag cgaatgagca
     1621 agatcataat cgccctgatg gttggcggca tacacacgca cctcaagcga aatgatcatc
     1681 ccccgtttga cacctagctc acgaaagatc tttaaacttt gctcagcaag ctctgctccg
     1741 cgagcatagt ttccctggtg aacagcgatt tcagccaacg cggcgatgat gcgcccaatt
     1801 ccacgcctat cgccgacctc ctgctttaac accaggcatt cattaagcag ggttgttgcc
     1861 tgcttaaaat agccctgatc ttttgcaaca gatcctaaat tgagcagtgc gtttgagata
     1921 cccagcttat cctttagttc acggtaaagg gataaagcct cctcaaatag agaaatcgcg
     1981 cgaacaaaat cgcttaacag ttgtgaaacc aaacccaaat tattcaaaga cagtgc
//
LOCUS       DBNHOH010000922         2355 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_379492, whole genome shotgun sequence.
ACCESSION   DBNHOH010000922 DBNHOH010000000
VERSION     DBNHOH010000922.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_379492"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="AC16V7_16640"
     CDS             <1..206
                     /locus_tag="AC16V7_16640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258803.1"
                     /translation="TLLMLDRQEIEAGGPMPPTKALKPELIDIISRWIVAGAPNTPEE
                     AAALTVRADTAIPEGTLPATTTP"
     gene            complement(394..777)
                     /locus_tag="AC16V7_16645"
     CDS             complement(394..777)
                     /locus_tag="AC16V7_16645"
                     /inference="COORDINATES: protein motif:HMM:NF013307.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     transmembrane subunit"
                     /protein_id="HIR6258804.1"
                     /translation="MTQLAEQIKTTTGNQAEVVAWKWMRYSGLLLIPLAWGHVAIQDV
                     LVGVHSIDLDYVAMRWASLAWRTYDFLLLAFAFAHGVNGLRQVIHDYIHNEKLKRKLN
                     WILIVFWLVVSAIGAIAIVGGVRLG"
     gene            complement(905..1309)
                     /gene="sdhC"
                     /locus_tag="AC16V7_16650"
     CDS             complement(905..1309)
                     /gene="sdhC"
                     /locus_tag="AC16V7_16650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HIR6258805.1"
                     /translation="MDSVRTTINGFASYRMRTGQLSFILHRLTGLGTLLFLAIHILDT
                     STVYFFPELYQHAIELYRSTPMMIGEIILVFSVIYHGVNGARIAIFDLFSPKWWQSGP
                     QERTALVTLVVAILLWLPAAFLMGRSLIIHNF"
     gene            complement(1577..>2355)
                     /locus_tag="AC16V7_16655"
     CDS             complement(1577..>2355)
                     /locus_tag="AC16V7_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005371124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HIR6258806.1"
                     /translation="LLKEHFPEDYAKIRFPDTAGIGLKPISKEGTARLVRAAIRWALE
                     NSRRSVTLVHKGNIMKFTEGAFRNWGYEVAEQEFGEHVYTWAQWERTKDAQGEDVANV
                     EQEAAVKSGQLLVKDVIADIVFQQTITRAREFDVIATMNLNGDYLSDALAAQVGGIGI
                     APGGNINYTTGHAVFEATHGTAPKYADKDMVNPGSVILSGEMMFNYMGWREVAQLIVK
                     GMEKAITAKTVTYDFHRLMEGATLLKCSEYGDAVIKHMQD"
BASE COUNT          602 a          662 c          565 g          526 t
ORIGIN      
        1 acacgctgtt gatgttagat cgccaggaga tcgaggcggg cggtccaatg ccgcctacca
       61 aagcactcaa gcccgagctg atcgacatca tctcgcgctg gattgtcgct ggagcgccca
      121 acacgcccga agaagccgcg gcgctcaccg tgcgagcaga cacagcaata cccgagggaa
      181 ccctgccagc tactacaaca ccatagcttc atcactgagc caatgccaaa atcatcgcca
      241 ttccatcgcc gtaaacactg ctgatccatc acagccgaca acactaccgc accgccgaac
      301 atcgcatcaa tttttcagca ggaagcgagg gatccctcgg attgcaaagc cccccaagta
      361 tggggggctt ttcttatcaa tcagaaagat ttattaccct aaccggacac cgccaacaat
      421 ggcaattgca ccgatcgccg acaccaccag ccaaaagacg atcagaatcc aattgagctt
      481 tctcttcaat ttttcgttgt gaatgtagtc atggatcacc tggcgcagac cattcacgcc
      541 atgggcaaag gcaaaagcca gcagcaagaa atcgtaggtg cgccaagcca gcgaagccca
      601 gcgcatagcg acataatcca gatcgatgct atgcacaccc accagtacat cttggatcgc
      661 gacatgtccc caagccaaag ggatgagcag caaaccgctg tagcgcatcc acttccaggc
      721 gacaacttca gcctgatttc cggttgttgt tttgatttgt tctgctagtt gagtcatttt
      781 ctgcttagat tcctaaaaaa taaaaaatca ttcctcatca aggtgatact cagtcgggct
      841 acactggatg gaccttagcg atcattctta gactaaaatg cgccatcacc caactacctg
      901 agcactaaaa attatgaatg atcaggctgc ggcccattag aaatgccgct ggtaaccaca
      961 gcaagatggc gaccaccagg gtaaccaggg cagttctctc ctgtggtcca gattgccacc
     1021 acttcggcga aaacaggtca aagatagcga tgcgcgctcc gttaactcca tggtagatca
     1081 cactgaaaac caggatgatc tcgccaatca tcatcggggt ggagcggtac agttcgatcg
     1141 catgttggta tagctccgga aagaaatata ctgttgacgt atccaggatg tggatggcca
     1201 ggaaaagcag cgtacccagg ccggttaagc gatgaaggat gaaacttaac tgaccggtac
     1261 gcatgcgata actggcaaat ccgttgattg tggttcgaac cgaatccatg agggagacgc
     1321 tcctgttcta aaagatataa ggaatgctaa tatactgttg ttttgattcg aaatatttta
     1381 ctatgtaatc attaaaccca tttaagatca gaacgggaac caattaattg aaggccaatt
     1441 ccaattaaga ggaagaaatc cctggcgatg cgctcgacga tctcctgctg gcagccattg
     1501 atctgcctga ggggcgggac accaagctgg ctctgatccg ctccgcggcc aagcctatgc
     1561 catcaaagta gattatctaa tcctgcatgt gtttaatcac cgcgtccccg tactcggagc
     1621 acttgagcag tgtggcgcct tccatcaggc ggtgaaagtc atatgtcacc gtcttagcag
     1681 tgatagcctt ctccataccc ttgacaataa gctgggccac ctcacgccaa cccatgtaat
     1741 taaacatcat ttcgccggat agtatgacgg aaccggggtt gaccatgtcc ttgtcagcgt
     1801 acttgggcgc tgtgccgtgg gtagcctcga atacagcatg cccggtggta tagttgatgt
     1861 tccccccggg cgcgatgcca atgcctccca cctgggctgc cagcgcgtcg gataggtaat
     1921 ccccgttcag gttcatggtg gcgatcacat caaactcgcg cgcccgggtg atggtctgct
     1981 ggaatacgat atcggcgatc acatccttga ccaacagctg gccagatttt acggcagcct
     2041 cctgctcgac gttagcaacg tcttccccct gggcgtcttt ggtacgctcc cactgcgccc
     2101 aggtgtaaac atgctcgccg aactcttgct ctgcgacctc atagccccag ttgcggaaag
     2161 cgccttccgt gaatttcata atgttaccct tgtgtaccag ggtcacgctg cgacgcgaat
     2221 tctctaacgc ccagcggatg gccgctcgta ccaagcgagc cgtcccctct ttggagatcg
     2281 gcttcaggcc gatcccggct gtatcaggga agcggatttt ggcgtaatct tccggaaaat
     2341 gctctttcaa taatc
//
LOCUS       DBNHOH010000923         1353 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_892597, whole genome shotgun sequence.
ACCESSION   DBNHOH010000923 DBNHOH010000000
VERSION     DBNHOH010000923.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_892597"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="AC16V7_16660"
     CDS             complement(<1..467)
                     /locus_tag="AC16V7_16660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6258807.1"
                     /translation="MYDLIVIGGGPAGLTATIYAIRKRLNVLLVSRDLGGKTNYRLSL
                     PWIEDYQVVRGLEVVNKFKSELEYLKFARLLQAVERVERRPEGFAVFTEEGRELLTRA
                     VIIATGTRQKFLSVPGERDFMMRGLCYSATSYAPLFIDRKTVVVGDGELALRST"
     gene            complement(490..>1353)
                     /locus_tag="AC16V7_16665"
     CDS             complement(490..>1353)
                     /locus_tag="AC16V7_16665"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0004473 - malate dehydrogenase
                     (decarboxylating) (NADP+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent malic enzyme"
                     /protein_id="HIR6258808.1"
                     /translation="KAEIPIWHDDQQGTATVTLAGLMNALKVVGKKKEEARIAFIGCG
                     AANVACSRLIYGWGVVPANCIMVDSKGILGMHRKDLELRKAEFVDKWRLCQITNAEGR
                     QGDIPAAMKGMDVVIALSRSGPGVILPEWVSSMEKDAILFACANPIPEIWPWEAREAG
                     ARVVATGRSDFPNQVNNSLGFPGIFRGALDVRAKTITDEMCYAASQALADQIGDRLDE
                     EHILPTMDDWEVYPREAAAVAMKAQEQGLARLQKSYDELLENAYAIIRRSRDLTQMMM
                     KQGFIPEAPAE"
BASE COUNT          286 a          422 c          349 g          296 t
ORIGIN      
        1 gttgagcgca acgccagctc gccgtcacca accaccacag ttttgcggtc aatgaaaagc
       61 ggtgcatagc tggttgccga atagcacagg ccacgcatca tgaaatctcg ctcacccggt
      121 acagaaagaa acttttgccg tgtccctgtg gcaatgatga ccgctcgggt gagcagctcc
      181 ctgccttcct cggtaaacac tgcaaagccc tccggacggc gctctacgcg ctcaaccgct
      241 tggagcaggc gggcgaactt gagatattcc aattcgctct tgaatttgtt gaccacctcc
      301 aacccgcgca ccacctggta gtcctcaatc cagggcagcg ataagcggta atttgtctta
      361 ccgcctaaat ctctggaaac cagcagcaca ttcaggcgct tgcggatggc gtatatcgtt
      421 gcggtgaggc ccgcagggcc tccaccgatc acaatcaaat cgtacatggg gcacctcgtc
      481 ctttttccat cactcagcgg gagcttcggg tatgaagccc tgcttcatca tcatctgggt
      541 gaggtcgcgc gaacgccgga tgatcgcata cgcattttcc agcagctcat cgtaactctt
      601 ctggagtcgc gccagacctt gttcctgagc cttcatcgcc acagccgctg cttcccgtgg
      661 atacacttcc cagtcatcca tggtgggcaa gatgtgttcc tcgtccagcc ggtcgccgat
      721 ctggtcagcc agcgcctgtg aggcagcgta gcacatctcg tccgtgatgg tcttggcgcg
      781 cacgtccaaa gcaccacgaa agatgcccgg gaaacctagc gagttgttta cctggttcgg
      841 gaaatcagaa cgccctgttg cgacaacacg cgccccagcc tctcgcgcct cccagggcca
      901 aatctcagga atggggttgg cgcaggcaaa caggatcgcg tccttttcca tgctggatac
      961 ccactctggt aagatcacac ctggtccaga gcgggagagg gcgatgacga catccatacc
     1021 cttcatcgcc gcgggtatat cgccctggcg accttctgca ttggtgatct ggcaaagtcg
     1081 ccacttatcg acgaactcag ctttgcgcag ctccaggtct ttgcggtgca tgcccaggat
     1141 gcccttgcta tccaccataa tacaattggc aggaacgaca ccccagccat agatgaggcg
     1201 agagcaggct acattagctg caccgcaccc aatgaaggca atcctggcct cttctttttt
     1261 cttccctacg accttgagag cattcatcag gcctgccagc gtaaccgtgg cagtgccctg
     1321 ctggtcatcg tgccagatgg ggatttcagc ttt
//
LOCUS       DBNHOH010000924         5249 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_957348, whole genome shotgun sequence.
ACCESSION   DBNHOH010000924 DBNHOH010000000
VERSION     DBNHOH010000924.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_957348"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..1375)
                     /gene="mgtE"
                     /locus_tag="AC16V7_16670"
     CDS             complement(14..1375)
                     /gene="mgtE"
                     /locus_tag="AC16V7_16670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR6258809.1"
                     /translation="MTENDAELNARRIRAALEIQRTDSAIAILYELRPADRADVFNML
                     SDTEQEILLPLLDIPATADLLEELEDQDALDAIGDFSPEKLGDVLDEMQPDEAADLLG
                     DLSPEQVTQALAHMEDADDVIPLLGYPDETAGGLMTTSFIALRRHTTAEQAIEFMRQV
                     ELETEVPYYLFVVDREKRLIGVIGLREIIVAQPSTTMETIMDREVVFVTVTTDQEEVA
                     RVISRYDLAAVPVVDESGRLIGVITHDDIVDVLEDETTEDILHLGAIESGPIIDKPYW
                     TQKMMDVVRSRFFWLLFLFVAETFTGTVLRHYEVELKTVVALTFFVPLLIGTGGNAGS
                     QTVATVIRALALREIRPKDAFRVWQRELKTGIVLGVMIGFVAIGGALLLGVTIELAIT
                     VGATITAIVVWANSVASLVPIVASRFGVDPTVLSGPLMATLIDGTGLLIYFSLAVLIL
                     PQL"
     gene            complement(1394..1612)
                     /locus_tag="AC16V7_16675"
     CDS             complement(1394..1612)
                     /locus_tag="AC16V7_16675"
                     /inference="COORDINATES: protein motif:HMM:NF020940.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HIR6258810.1"
                     /translation="MSDQIYRLIYPVSLLSVPVINQLIRRYDITVNILRAHIGGENRW
                     MEVQLTGDPMVIEDAIHWLKDQGIEVTY"
     gene            complement(1640..2683)
                     /gene="rlmN"
                     /locus_tag="AC16V7_16680"
     CDS             complement(1640..2683)
                     /gene="rlmN"
                     /locus_tag="AC16V7_16680"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual-specificity RNA methyltransferase RlmN"
                     /protein_id="HIR6258811.1"
                     /translation="MNEREQQFIFDIDYEGLVSIIESWGEPLYRAQQIWQGLYKNLWN
                     EPDHFTTLSSKLRKKLAEEFTFYQIEPVQVLTSGDKETEKTLFRLSDQRAIEAVLMRY
                     NKRRTLCISTQSGCAMGCVFCATGQMGFQRNLSSGEIVEQVIYFARKLQAQDDKVSNV
                     VIMGMGEPFHNYDATMQAIERLNHPEGMGLGARRFTISTVGLVPAIKRFTAEHRQINL
                     AVSLHASDDDLRSSMLPINKKYPISELIQTCRDYVKKTRRRITFEWALIRDVNDSIDQ
                     ARQLAELLQGMLCHVNVIPLNPTQRYTGQATTRQRALAFKSELDKRGVPCSIRLRRGI
                     DIQAGCGQLAIEV"
     gene            complement(2687..3481)
                     /locus_tag="AC16V7_16685"
     CDS             complement(2687..3481)
                     /locus_tag="AC16V7_16685"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HIR6258812.1"
                     /translation="MKTPTTADIEALILDMDGVLWRDDQPIGNLKAIFSQIQQQRWQV
                     TLATNNATRSVEQYLNKLKGFGVSLNPDQIVNSAQAVVHYLTINHPLGGNVYVIGEEG
                     LLHTLAEDNFQHSLENPIAVIVGMDRELTYKKLRQATLLIRAGIPFIGTNPDRTFPTP
                     DGLVPGAGAILAALEASTSISPIIVGKPSPEMYMVAMDRMGIKTEATLVVGDRLETDI
                     AGAQKLGCPTALVLSGVTSWQAAQSWSPRPEWIEPDLASLLNRLVG"
     gene            complement(3487..4359)
                     /locus_tag="AC16V7_16690"
     CDS             complement(3487..4359)
                     /locus_tag="AC16V7_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018461587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HIR6258813.1"
                     /translation="MLHPSTFSIVMYDAKEKAWGIVVASKFLAVGSVVPWARANAGAV
                     ATQSFANTTYGNRGLTLMEAGHSAEETLALLLDDDPERELRQVGLVDAQGRSATYTGS
                     SCFVWAGGLSGDGFAIQGNILAGEQVILAMEASLRENTEKTIIWRLYNALLAGDQAGG
                     DRRGKQSAAILVVKAQAGYAGFNDRWVDYRVDDHHEPVKRLGELLNLHELYFGKSPLE
                     ERVRLVGEPLRQLQDIMAKFGYYRGRFHGEYDAPTQTALRAFIGNENFEDRTNFDAGL
                     IDRPVLEHLLSRFG"
     gene            complement(4436..5050)
                     /locus_tag="AC16V7_16695"
     CDS             complement(4436..5050)
                     /locus_tag="AC16V7_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HIR6258814.1"
                     /translation="MVTNPLSLIPTVIETSGRGERYFDIYSLLLRNRIVFLGTPINDQ
                     ISNLIVAQLLYLSSEDPEAPIQMYINSPGGQVYAGMAIYDTMQMIPNPLSTLAVGMTA
                     SFGTILLTAGTKGQRYALPNATIHMHQPLGGAQGQASDIEIQAREILRLKDKLNEILA
                     KHTNQSVETIEKDTNRDFYLDAEGAVEYGLVDQVMELPNPKQSG"
BASE COUNT         1360 a         1335 c         1108 g         1446 t
ORIGIN      
        1 ctgtacgatt caatcatagc tgcggcagaa ttagaacagc caaagagaaa tatattaaca
       61 atccagttcc atcaattaag gtcgccatta gagggccaga aagcacagtt ggatcaaccc
      121 caaatcgact agcaacaatt ggtaccaggg aagccaccga atttgcccat acaacgatcg
      181 ccgtgatggt tgcacctacg gttatggcta actcaatcgt cacacctaac aatagcgccc
      241 cgcctatggc aacaaaacca atcattaccc caagaacgat gccggttttt aactctcgct
      301 gccatacacg gaatgcatct ttggggcgta tttcgcgcaa ggctagcgcg cggattaccg
      361 ttgcaacagt ttgagaccct gcattcccac ccgttccaat caacagcgga acaaaaaacg
      421 ttaaggcaac cactgttttt aattcaactt catagtgacg caataccgtg ccagtaaatg
      481 tttcggcaac gaataagaag agcagccaaa aaaatcggga acgaaccaca tccatcattt
      541 tttgagtcca atacggtttg tcaatgatgg gtccagattc tatagctccc aagtgaagaa
      601 tatcttcagt ggtttcatct tctagcacat ctacgatatc atcgtgcgtg atcacaccta
      661 tcaaacgtcc tgattcgtcc accactggca cagcagccag atcataacga gatattactc
      721 gggcgacttc ctcttggtca gtagtcactg tgacgaatac aacttcgcga tccatgatag
      781 tttccatcgt agtagatggt tgcgcaacta tgatttcgcg taagccaatc actccaatca
      841 gtcttttttc tctatcaaca acgaacaggt agtacgggac ttcagtttcc agctccacct
      901 gacgcataaa ttcaatcgcc tgctcagcag ttgtatgcct tcgcaaggcg ataaatgaag
      961 tggtcatcaa accaccggcc gtttcatcag gatagccgag caatgggata acatcatcgg
     1021 catcttccat gtgcgccagt gcttgggtga cctgttctgg tgaaagatcc cctaataaat
     1081 ctgctgcctc atcaggctgc atttcatcta gaacatcacc cagtttctct ggtgaaaaat
     1141 ctcctattgc atccagagca tcctgatcct ctagctcttc caaaaggtct gcagttgccg
     1201 ggatatctaa caaaggcagc aagatctcct gctctgtatc actcagcata ttgaagacat
     1261 cggctcgatc tgcagggcgc aactcgtaga ggattgctat tgcgctgtca gttcgttgaa
     1321 tctcaagcgc agctcgaatc cgtcgagcgt ttaattcggc atcgttttca gtcatgattt
     1381 tttatactcg aaattagtat gtaacttcga tcccctgatc tttcagccaa tggatagcat
     1441 cttcgataac cataggatca ccggttaatt gaacttccat ccagcggttt tcccctccaa
     1501 tatgtgcacg taatatattg acggttatgt cgtagcgacg gatgagctga ttgattaccg
     1561 ggacactcag taaagaaact ggatagatga gtcggtaaat ctgatcggac atagcaacct
     1621 ccaaactttt gaaacttgcc tatacttcta tcgctaattg cccgcaccct gcttgaatat
     1681 caatgcccct ccgcaggcga atggagcagg gaaccccacg tttgtccagc tctgacttaa
     1741 aggcaagggc gcgttggcga gtcgtcgctt gacctgtata cctctgagtt ggatttagtg
     1801 gtatcacatt cacgtgacat aacattcctt gcagtaattc agccagttgc cttgcctggt
     1861 caatactatc attaacatcc ctgatcaacg cccattcgaa cgtgatcctt cgacgggttt
     1921 tcttcacata atctcgacag gtttggatca attcgcttat cggatatttc ttattgattg
     1981 gcaacatcga agaacgaagg tcatcatcgg aggcgtgtaa tgatactgcc aaattgatct
     2041 gcctgtgttc agcagtgaag cgctttatgg ctgggaccaa gcctaccgtc gaaattgtaa
     2101 atcggcgcgc acccaacccc atcccttcag ggtgattcaa ccgttcaatc gcttgcatcg
     2161 ttgcatcata gttatgaaaa ggttcaccca tacccatgat gaccacattg gacactttat
     2221 catcttgtgc ctgtaatttc cgagcgaaat aaatgacttg ttcaacgatc tcgccactac
     2281 ttaaattgcg ttgaaatccc atctgtccag tggcgcaaaa aacacatccc atagcgcaac
     2341 ccgactgagt agatatacac agcgtccgac gcttattata gcgcatcaat accgcctcga
     2401 ttgcgcgctg atcagaaaga cgaaagagcg tcttctcagt ctctttatcc ccagaagtaa
     2461 gaacttgaac cggctctatt tggtaaaatg taaactcttc agccagcttt ttacgcagct
     2521 tgctagacaa tgtcgtaaaa tgatctggtt cattccagag atttttatac aagccctgcc
     2581 agatttgttg cgcccggtat agaggctctc cccatgattc aattatagat actaaacctt
     2641 cgtagtcgat atcaaatatg aactgctgtt ctcgctcatt cattttttat cctactaaac
     2701 ggttcaataa agaagccaga tctggttcaa tccactccgg acgtgggctc caggattgag
     2761 ccgcttgcca gcttgttacc ccagatagca ccagtgctgt tggacaacca agtttctgcg
     2821 cccctgcaat atcagtttcc agacgatcac cgacaaccaa tgtagcttct gtttttatac
     2881 ccattctatc cattgctacc atgtacattt caggcgaggg tttgccgaca ataatgggcg
     2941 agatagatgt agaagcttcc agagcggcaa gaattgcgcc tgctcccgga accagaccat
     3001 caggagtggg gaatgtgcga tccgggtttg taccgataaa tgggatgcct gcccgtatca
     3061 acaaagttgc ttggcgcagc tttttatatg tcaattcccg atccattcct acgatcaccg
     3121 ctattgggtt ttcaaggcta tgttgaaaat tatcctcagc cagagtatgc agcaacccct
     3181 cttctccgat gacatacaca ttgccaccca aggggtgatt tatggttaag taatgaacca
     3241 ccgcttgggc tgaattgact atttgatctg ggttgagtga tacgccaaaa cctttaagtt
     3301 tattaagata ttgttctact gagcgcgttg cgttgttcgt ggcgagagtt acctgccatc
     3361 tttgctgttg gatttgggaa aatattgcct tcaaattgcc aataggctga tcatcccgcc
     3421 acaatactcc atccatgtcc aggatcagcg cttcgatatc agcggttgtg ggtgttttca
     3481 tagagtttat ccaaaccggc ttaacaggtg ttccaagaca ggtcggtcga tcaatcctgc
     3541 atcgaaattt gtgcggtctt caaagttttc attgccaata aatgctctca gagcggtctg
     3601 ggttggagcg tcgtattctc cgtggaaacg accccggtaa tacccgaatt tcgccataat
     3661 atcttgaagc tgacgcaggg gttcgccaac caggcgaaca cgctcctcta agggactttt
     3721 tccaaagtaa agttcatgca gatttaaaag ctcacctagt ctcttgacag gttcatggtg
     3781 gtcgtccaca cgataatcca cccaacgatc attgaagcca gcatagccag cttgagcttt
     3841 gaccaccaga atggctgcgc tttgtttgcc gcgtcgatct cctccagcct ggtcgccggc
     3901 tagcagagca ttatacaagc gccaaataat tgttttctct gtgttttccc ttaatgatgc
     3961 ttccattgca agaataacct gttctccggc gagtatatta ccttgaatcg cgaaaccgtc
     4021 accactcagg ccgcccgccc acacaaaaca ggagctccca gtatatgtag ctgatctccc
     4081 ttgggcatca actaacccaa cctgtcgcaa ttctcgctcg gggtcatcat ccaatagtaa
     4141 cgctagggtt tcttcagcag aatgccctgc ttccatcaaa gtcaatcctc gattcccata
     4201 agtggtgttc gcgaaagatt gtgtagcaac tgcacctgca ttagcacgag cccaaggcac
     4261 taccgaaccc acagccagga attttgatgc cactactatc ccccaggctt tttctttcgc
     4321 atcgtacatc acgatagaga atgtcgacgg atgaagcata aatacctcct gataatattc
     4381 cccctccatg cagcaatttg catggagggg gaatgatctc agtggtcaga gtgttttacc
     4441 cactttgttt ggggttaggt agctccataa cctgatccac caaaccgtac tcaacagctc
     4501 cctcggcatc gagatagaaa tctctgttcg tatctttctc gattgtctca acactctgat
     4561 tggtatgctt ggctaaaatt tcgtttaact tgtccttcag gcgaaggatt tcgcgtgctt
     4621 gtatttcgat atctgacgct tgtccctggg cgccacctaa cggctgatgc atgtggattg
     4681 tggcatttgg gagcgcgtat cgttgacctt ttgtccctgc agttaggaga atcgttccaa
     4741 aggaagctgt catgccgact gcaagggtac ttagtgggtt aggtatcatt tgcatcgtat
     4801 catatattgc catcccagca tatacctgcc ctccaggcga gttaatatac atctgtattg
     4861 gagcctctgg gtcttcgctg ctcaggtaca gcagttgggc gacaattaaa tttgatatct
     4921 gatcattgat aggagtgccg agaaatacga tgcgattccg caacaataac gaatagatat
     4981 caaaatatcg ttcaccgcgt cccgaggttt ctattaccgt agggattaag gaaaggggat
     5041 ttgtaaccat atactatttg cctctcttcg atacacaaaa ttatgcgatc aaatcactgt
     5101 ttgtccccag aattatgggg ctagttattc tcttcgtctt ctccgtccac cgtttccgaa
     5161 gaagcatttt cactttctac ctcggcggca ggtttttcag tatcatcagt ttcctgaagt
     5221 tgatcactag atttaggctc taatccacg
//
LOCUS       DBNHOH010000925         3719 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1661605, whole genome shotgun sequence.
ACCESSION   DBNHOH010000925 DBNHOH010000000
VERSION     DBNHOH010000925.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1661605"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..2889)
                     /gene="acnA"
                     /locus_tag="AC16V7_16700"
     CDS             complement(190..2889)
                     /gene="acnA"
                     /locus_tag="AC16V7_16700"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HIR6258815.1"
                     /translation="MHKDYFNARAVLKTSHGDYIFFHLGRLEKEGLTQLERLPFSIRV
                     MLESLLRQCNEREITRQDVINLAGWTAQAAERPTLPYRPARVIMQDLTGVPAVVDLAA
                     MRSAVSRLGGDPKQINPLVPVDLVIDHSVQVDFFATPDALMRNADLEFQRNQERYEFL
                     RWGQQAFDNFRLVPPATGIVHQVNLEYLADVVMANDEDGERIAYPDTLVGTDSHTTMI
                     NGLGVVGWGVGGIEAEAAMLGQPIDMLTPDVVGFKMEGQLNEGVTATDLVLTVTQMLR
                     KKGVVDKFVEFFGSGLDSMSLTDRATIANMAPEYGATIGFFPVDDETMRYLRLTGRSP
                     EVIERVERYTKEQGLFRTTHTPDPEFTDTLELDLSSVEPSLAGPKRPQDRIPLREMKR
                     AFRSALTAPVKERGYELEPEALERSAAMGTNGGSVEMGHGSVVIAAITSCTNTSNPAV
                     MIGAGLVAKKAVERGLQVRPYVKTSLAPGSRVVTDYLSKAGLLVSLEELGFNVVGYGC
                     TTCIGNSGPLSGEVVKAISGADLVAAAVLSGNRNFEGRVHPFVRANYLASPPLVVAYA
                     LAGTVDIDLVNDPLGVDRDDQPVYLRDIWPSTQEIQDTIAQAVLPEMFAEKYADVFSG
                     TQTWKDIQASQGELFEWNPSSTYIQEPPFFINLTREVGKIQDIHAARVLAVMGDSITT
                     DHISPAGAIPANAPAGLYLIDQGVSVRDFNSYGARRGNDRVMTRGTFANIRLKNRMVP
                     GVEGGVTLHLPDHVQMSIYEAAMSYQKENVPLIVLAGKEYGTGSSRDWAAKGTLLLGV
                     RAVIAESYERIHRSNLVGMGVLPLQFLPGENVDTLGLTGSEKFFIEGLSDQIIPNGEV
                     VVRAVRENNGQVIFKAIARLNTPVEVDYYRNGGILHTVLRSML"
     gene            complement(2949..>3719)
                     /gene="folP"
                     /locus_tag="AC16V7_16705"
     CDS             complement(2949..>3719)
                     /gene="folP"
                     /locus_tag="AC16V7_16705"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR6258816.1"
                     /translation="DTGDKWLQTALELARGYVADGADLLDVGGESTRPGAETVSLEEE
                     LERVLPVIRQLASEIDVPISIDTYKAEVAEAALEAGAHMVNDVWGFKVDPRLAEVAAR
                     HKVPVILMHNRSSWANAEIQARLGGRYVGVPYENLLEDIRRELMESVAIARSAGIPDE
                     HIILDPGIGFGKTVEQNLELLDRLGEIRALGFPIMLGPSRKSFIGYTLNLPPDQRVEG
                     TAAAVAIGIARGADIVRVHDVKSMARVTRMGDAIVRRG"
BASE COUNT          826 a         1087 c         1013 g          793 t
ORIGIN      
        1 agggaaggat gtcaatataa ggagtttcca cctcctacca gcattttttc accgccggtc
       61 ccctcaccgc atgcggagag ggggagaaaa acccaaaaac aggtgtttgc atgggcacaa
      121 cccaagcaaa cacctgtttt ctgatcaata agcccttccc ctatcctggg taaggggtgc
      181 aggttctgtt cacaacatac ttcttaggac ggtatgcaag ataccgccgt tgcgatagta
      241 atccacctcg acaggggtgt tcaagcgggc gatggcttta aatatgacct gaccattatt
      301 ctctctcact gcccgcacaa cgacctcgcc atttgggatg atctgatcac tgagtccctc
      361 gatgaaaaat ttctcgctgc ccgtcaggcc aagcgtatcc acgttctcac caggtaaaaa
      421 ttgcaagggg aggacgccca taccaactag attagagcgg tggatacgtt cgtaagactc
      481 agcgataacc gccctgacgc ccaatagcag ggtgccctta gccgcccagt cgcggctgga
      541 accggtgcca tactccttgc ctgccagtac gatcaggggc acattctcct tctggtagct
      601 catggcagcc tcgtaaatgc tcatctggac gtggtcgggc aagtgcaggg tcacgccgcc
      661 ttccacaccg ggcaccatgc ggttcttaag acgaatattg gcgaatgtgc ctcgggtcat
      721 cacgcggtcg ttgccgcgcc gggcaccata cgagttgaag tctcgcacgc tcaccccttg
      781 gtcgatcagg tacaaacctg caggagcgtt ggccgggatg gcacccgccg gggagatgtg
      841 atcagtggta atcgaatcgc ccatcactgc caggacacgc gccgcgtgga tatcctggat
      901 cttgccaacc tcccgcgtta ggttgataaa gaatggtggc tcctgaatat aggtagatga
      961 cggattccac tcgaacaatt cgccctggct ggcttgtata tctttccaag tctgggtacc
     1021 gctgaagaca tcggcgtatt tctcggcgaa catctcgggc agcacagcct gtgcgatggt
     1081 gtcctgtatc tcctgtgtcg atggccaaat atcgcgcaga tacaccggct ggtcatcacg
     1141 gtcaacaccc aaaggatcat tgaccagatc gatgtccacc gtccccgcca gggcataggc
     1201 aaccactaat gggggggagg cgaggtagtt ggcgcgcacg aaggggtgca cccgtccttc
     1261 aaagttgcgg tttcccgaga gcaccgctgc agcaaccaga tcagcgccgg aaatcgcctt
     1321 gaccacctcg cctgagagcg gacccgagtt tccaatacaa gtggtacagc catatccgac
     1381 gacattgaag cccagctcct ccagcgagac gagcaggccg gctttgctta gataatcggt
     1441 caccacgcgc gagccaggcg ccaggctggt cttgacatag ggcctgacct gcaggccgcg
     1501 ctcgacagcc ttttttgcta ccaggccggc tccgatcatc accgctggat tggaggtgtt
     1561 ggtgcaagag gtgatagcag cgatgacgac agaaccatgc cccatctcta cactaccccc
     1621 gttggtgccc attgccgctg agcgttctag tgcttctggt tccagctcgt agccacgctc
     1681 tttgactggg gcggtgagtg cgctacgaaa tgccctcttc atctctctaa gcgggatgcg
     1741 atcttgaggg cgtttaggtc cggcaaggct gggttctaca ctgctcaggt cgagctccaa
     1801 cgtatcggtg aactcaggat caggtgtgtg ggtggtgcga aacagcccct gctctttggt
     1861 atagcgctcg acgcgctcaa ttacctcggg ggatcgcccg gtcaggcgca aatagcgcat
     1921 tgtctcatca tccacaggga agaagccgat ggtggcgccg tattcgggag ccatattggc
     1981 gatggtggca cgatccgtca gagacatcga gtccagccca gagccgaaga actccacgaa
     2041 tttatccaca acccctttct tgcgcagcat ctgggtgaca gtcagcacca ggtcggtggc
     2101 ggtaacccct tcattgagtt ggccttccat cttaaatccg accacatctg gcgtcagcat
     2161 atcaatcggc tggcctagca tagcagcctc ggcttcgatg ccgcctacac cccagccgac
     2221 cacacccagg ccattgatca tggtggtgtg cgagtcggta ccgaccagtg tgtccgggta
     2281 ggcaatcctc tcaccatcct catcatttgc catgaccaca tctgccaggt attccaggtt
     2341 aacctggtgg acgataccgg tggcaggtgg caccaagcga aagttatcaa aggcttgttg
     2401 tccccagcgc agaaattcat agcgttcttg attgcgctgg aattccaaat cagcgttgcg
     2461 catcagcgcg tccggtgtgg cgaaaaagtc cacttgaacg gaatggtcaa tgaccaaatc
     2521 aaccgggacg aggggattga tctgtttggg atcaccaccc aggcgcgaca cagccgagcg
     2581 catagctgct aggtcgacaa cggctgggac accggtcagg tcttgcataa ttacacgggc
     2641 ggggcggtag ggcagcgtgg gccgctctgc ggcttgtgcc gtccagccag ccagattgat
     2701 cacatcttgg cgagtgatct cacgctcgtt gcattgcctt agcagagatt cgagcataac
     2761 acggatggaa aaaggcaagc gctccaattg ggtcaaacct tccttttcca gacgacccag
     2821 gtggaaaaag atgtagtcac cgtgagacgt tttgaggacc gcgcgagcat tgaaataatc
     2881 tttgtgcatg aacatttcct tgcaagagga tatttgaaaa agctaattgg ggattgagat
     2941 tataccatct atccccgtct gacgatggcg tcccccattc ttgtcacacg agccatagat
     3001 ttcacgtcgt ggacgcggac aatatccgcc ccacgggcga taccgattgc gacggctgcg
     3061 gcggtaccct ccacacgctg atcaggggga agattcaagg tgtagccgat gaacgacttg
     3121 cgcgatggcc ccagcattat agggaagcca agtgcgcgga tttcccccaa gcggtccaaa
     3181 agctccagat tctgctccac agtcttccca aagccaatcc cagggtcaag gataatgtgc
     3241 tcgtctggta ttccagccga cctggcgata gctacgcttt ccatcagctc gcgccggata
     3301 tcttccagca agttctcgta aggaacaccc acataacgcc cccccagtcg ggcttgaatc
     3361 tcagcatttg cccacgagga gcggttgtgc atgaggatga cgggcacctt gtgacgggct
     3421 gcgacttcgg ccaagcgcgg gtccactttg aacccccaga catcattgac catgtgcgct
     3481 cctgcttcca gggcagcctc cgccacctca gccttgtagg tgtcaatcga gatgggaaca
     3541 tcgatctccg atgccagctg gcggatgacc ggcagaacgc gctccaattc ctcttcaagg
     3601 cttacagttt ccgcgccagg acgcgtgctc tcaccgccca catcgagaag gtcagctcca
     3661 tcagcgacgt agccgcgagc gagctccagg gcagtctgta gccacttatc ccctgtatc
//
LOCUS       DBNHOH010000926         1789 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1533876, whole genome shotgun sequence.
ACCESSION   DBNHOH010000926 DBNHOH010000000
VERSION     DBNHOH010000926.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1533876"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..1494
                     /locus_tag="AC16V7_16710"
     CDS             184..1494
                     /locus_tag="AC16V7_16710"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HIR6258817.1"
                     /translation="MNEIKRVYVMQQIEDKQLTGPQAAERLDLSLRQIRRLLAKYRTQ
                     GAPGLVHGNRGRVANNRIEEAVRAKIWELAQEDYKVYNDSHFTEELVEEYGLEVSRSS
                     VRRIRRENGQPSPRKRRSPRHRSRRARKAQAGMLLQTDGSRHDWLEGRGPWLTLIAYI
                     DDATNQVSGATFRDEEDAVGYFLGLQEICLRQGIPAAIYADRHTIFQSPTKATLEQEL
                     AGEQPRSQLGRLLDELGIELIAAHSPQAKGRVERLWETLQDRLVKALRKAGAADREGA
                     NQVLADFLPKFNQRFQVAPAQTGTAYVPWPEAYRPEDYFCFKHTRTVTNDNTLSFDGH
                     CLQIPPGPQRRSYAKAKVDVRQHLDGWLEVRYQGHSLVTFEPIDERPVRIQKFNPAPG
                     QVAPAQQQGALKTVKKPKVRKPYKPAADHPWRRPLFAKPKERKE"
BASE COUNT          493 a          464 c          464 g          368 t
ORIGIN      
        1 gatcctggag ctcttcaggg agttgatcat cttgcataca aattgtctcc ggaccgcata
       61 ataacactgc ctatccaaat cgtcaactgc ccagatcagt gatttgttat tgttcagcga
      121 aattgacacc cactaactgt tcagcgaaaa tgtcacccta tgaacaagag agtgacattc
      181 agcatgaacg aaatcaaacg tgtgtacgta atgcagcaga tcgaagataa gcaattgaca
      241 gggcctcaag ccgctgaacg gctggatcta tctctgcgcc aaatccgccg cttgcttgcc
      301 aaataccgga cacaaggagc acctgggttg gtacatggca atcggggacg ggtagccaat
      361 aaccgcatcg aggaagcagt gcgggccaag atctgggaac tggcgcagga ggattacaag
      421 gtttataacg acagccattt cacggaagaa ctggtcgagg agtatggcct ggaagtgtcc
      481 cgctccagcg tacggcgcat tcgtcgagaa aacggccagc cgagccctcg caaacgccgc
      541 agtccccggc atcgcagccg gagagcgcgc aaagcgcagg ctgggatgct cttacaaacg
      601 gatggcagtc gccatgactg gttggaaggg cggggaccct ggctgacctt gatcgcttac
      661 atcgacgatg ccaccaacca ggtgtctgga gctacttttc gggacgaaga agatgcagtt
      721 ggatattttc tggggttaca ggagatttgc ctgaggcagg gcatccccgc agccatttat
      781 gccgaccggc acaccatctt ccagagcccc acgaaggcca ccctagaaca agaactggcc
      841 ggagaacaac ccagaagtca gttgggccgt ctgctggacg aactggggat tgaactgatc
      901 gcagcccaca gcccacaagc aaagggccgc gttgagcggc tgtgggagac gctccaggac
      961 cggctggtca aagccttgcg taaagcgggg gcagctgatc gagaaggagc caaccaggta
     1021 ttagcggact ttctgcccaa gttcaaccag cgtttccaag tagcgcccgc tcaaacaggg
     1081 actgcctatg tgccctggcc agaagcatat cggccagaag attacttctg cttcaagcac
     1141 acccgcaccg tgaccaacga caataccctc tccttcgatg gtcactgcct gcagatcccg
     1201 cccggccccc agcgcagaag ttacgctaag gccaaagtcg atgtcaggca acacttagat
     1261 ggttggctag aagtgcgcta ccagggacac agcttggtca cctttgagcc tattgatgag
     1321 cggcctgttc gcatacagaa gttcaaccct gctcccggac aagtggctcc cgctcagcag
     1381 caaggggcac tgaaaacagt gaagaaaccg aaagtgcgta agccctataa accagccgcc
     1441 gaccacccat ggcgaagacc gctttttgct aaacctaagg aaaggaaaga ataatccaag
     1501 ataaatgaca gagaccacaa aaaagacgag agggaacttt tgtaagcatc ccttctcgcc
     1561 ttcctttggg ataaagccga ttatacttga gcgatgatct aattcagggt gacatttctg
     1621 ttgaacaatt aaggtgacag aatcgctgaa caatgacata tcaatggtgg atttaaattt
     1681 ggagaactag tggatatcca attccttgga tgcttcttat gatttaccca tagatttcta
     1741 aatttgaatt taatattaat tgaggttgca attcaataca ctgtaaatt
//
LOCUS       DBNHOH010000927         1674 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_316120, whole genome shotgun sequence.
ACCESSION   DBNHOH010000927 DBNHOH010000000
VERSION     DBNHOH010000927.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_316120"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="AC16V7_16715"
     CDS             <1..849
                     /locus_tag="AC16V7_16715"
                     /inference="COORDINATES: protein motif:HMM:NF013828.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6258818.1"
                     /translation="EEIRRAPLLILDDFGTQNATAWSQEKLFQIFNFRYINRLPLVVT
                     TNTPLEDIEGRIRSRLEDPDLVTKVQILAPDFRRPAGDTGQNRVSSLDLLYKCTFANF
                     DLREGEGLKPPDLKSLKEAFRVARRFAEHPQGWLVFSGPNGCGKTHLAAAIGHHRRDL
                     GDRVVFTVVPDLLDHLRATFNPQSTVRLDRRFEEVRTAPVLILDDLGTQAATPWVREK
                     LYQLFNYRYNAELPTVITTADYVRDVDPRLLSRMEDRRLCEISAITAPAYRGVQQKPG
                     RRTARR"
     gene            973..1161
                     /locus_tag="AC16V7_16720"
     CDS             973..1161
                     /locus_tag="AC16V7_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258819.1"
                     /translation="MTTNQSACIYCERDHTQVPLLQIQYQGNVYWICPQHLPVLIHKP
                     AQLAHKLPGAENLDPGVH"
     gene            complement(1184..>1674)
                     /locus_tag="AC16V7_16725"
     CDS             complement(1184..>1674)
                     /locus_tag="AC16V7_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HIR6258820.1"
                     /translation="LLVSSNPGDIVADFFCGSGTTALVAARLDRRFIANDVTWRAVHT
                     TKTRLVAEAKELFSISNLEGRKVETADLPSASFEVTQTGNVVSIEAGEDIDLDYWEVD
                     PAWDGRVFSSAAQAVRPRRKGTIPLELMLPDSSKPSGSPGGYPRCARLVTTTGEQYQI
                     LF"
BASE COUNT          384 a          510 c          419 g          361 t
ORIGIN      
        1 gaggagatcc gccgcgcccc tttgctcatc ctggacgact ttggcaccca gaacgccacc
       61 gcctggtcgc aggaaaaact cttccagatc ttcaatttcc gctacattaa ccgcctgccc
      121 ctggttgtga ccaccaacac ccccctggaa gacatcgagg gtcgcatccg ctctcgcctg
      181 gaagatccag acctggtcac gaaagtgcaa attctagccc ctgatttccg ccgtccagct
      241 ggggataccg gccagaatag ggtctcatct ttagatttgc tatacaaatg cacttttgcc
      301 aatttcgacc tgcgcgaggg cgagggcttg aaaccccctg acttaaagag cctgaaggag
      361 gccttccggg tcgcgcggcg ctttgccgaa cacccccagg gctggctggt gttctctgga
      421 ccaaatggct gcggaaagac ccatctggcg gctgcgattg ggcatcaccg tagagacctc
      481 ggagaccgcg tcgtattcac tgttgtgccc gacttgcttg accacctgcg agcgacattc
      541 aacccccaaa gcacagtgcg tttggatcgc cgcttcgaag aagtgcgcac cgctcctgtg
      601 ctgatactgg acgacctggg cacgcaagcc gccaccccct gggtgcgaga aaaactatac
      661 cagttgttca actaccgcta taatgccgag ctgcccacgg tgatcaccac tgccgattac
      721 gtcagagatg tagatcctcg cttgctcagc cgcatggaag accgtagact atgcgagatt
      781 agcgccatca ccgccccggc ctatcgcggc gtacaacaga agccagggcg acgcacagcg
      841 cgcaggtgac aatttcaccc caaatactga gatactgaga gcaaattgaa tctctgccct
      901 aatcctctat ttgtgctgag aataattcgc tgaatctacg agaaaaattt atcgtaaaca
      961 atggagttta atgtgacaac caatcaatcc gcttgcattt actgtgaacg cgaccatacc
     1021 caggtgcccc tcttgcagat ccagtaccag gggaatgtct attggatttg cccccaacac
     1081 ctgcccgtgc tcatccacaa gcccgcccaa ctggctcaca agctaccggg tgctgaaaat
     1141 cttgaccctg gagtacatta agcgggtctc cactttcctt cgcttagaac aaaatctggt
     1201 attgttcgcc tgtcgtggtg accaggcgag cgcagcgcgg atacccgcct ggcgacccag
     1261 aaggtttgct gctatcaggt agcatgagct ctaagggaat agtgccttta cgccgcggtc
     1321 ggacggcctg cgctgcactg gagaagaccc tcccgtccca ggctggatcg acctcccaat
     1381 aatccagatc gatatcctct ccagcttcga tagagaccac gttgcctgtc tgagttactt
     1441 caaaggaagc agatggcagg tcggctgtct caaccttccg tccctcgaga tttgatatgc
     1501 tgaataactc ttttgcctct gccaccagcc tggtctttgt agtgtgcact gcccgccagg
     1561 tgacgtcatt ggcgatgaag cgcctgtcca gacgcgccgc taccagggct gtggtacccg
     1621 agccgcagaa gaaatcagcc actatatctc caggattgga agataccagc aaga
//
LOCUS       DBNHOH010000928         1630 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_443527, whole genome shotgun sequence.
ACCESSION   DBNHOH010000928 DBNHOH010000000
VERSION     DBNHOH010000928.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_443527"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="AC16V7_16730"
     CDS             complement(<1..330)
                     /locus_tag="AC16V7_16730"
                     /inference="COORDINATES: protein motif:HMM:NF044287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nSTAND1 domain-containing NTPase"
                     /protein_id="HIR6258821.1"
                     /translation="MSDKPNPTANPFVGPRALERGEAIFGREAETRKLSNLLIANRII
                     MLYSPSGAGKTSLIQAAMMPRLGGRNFDVLPAMRVNLTPPDELVSLPGFNRYVFSALT
                     ALEEGRSS"
     gene            complement(334..>1630)
                     /locus_tag="AC16V7_16735"
     CDS             complement(334..>1630)
                     /locus_tag="AC16V7_16735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024685.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - histone deacetylase activity,
                     NAD-dependent [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIR2 family NAD-dependent protein deacylase"
                     /protein_id="HIR6258822.1"
                     /translation="DMPMLVVLASCDSAKAEAGQAFSALGPRLSQAGVPAVLAMQGKF
                     SMLTNARFTPAFFEALQETGVVDLAVSLARGQVRDRPDHWMPALFMRLRSGRIWYTPG
                     LGSEASDIETWESLKTHIQEEMCTLLLGPEIVRSIQGDEREIALQWSELHHYPLCKED
                     RDKLPRVAQFVSVFQKESHVRIQFQKALRQALERGFPNAIPTELAQLERWKPEHLLQA
                     LVLAEAAQWSRADDNPYRLLANLGLPIYLTSERSDLLQNALREAGREPQVRLCPWKDS
                     LLNRRQLWEYNDDPTPEKPLVYHLFGHFKEPDSLVYTEDDIFDYLIKLTEHRDLMPPH
                     AIRAALADSSLLFVGFQFDDWQFRVITRLIMNQPGAEGLRVYPHVAAQIAPREGLIED
                     TEMARKYIQRYLKEGQDARIDLYWGTSQDFLKELNQYMR"
BASE COUNT          314 a          473 c          506 g          337 t
ORIGIN      
        1 gcttgaccgg ccctcttcca gggcggtcag cgcactgaac acatagcggt tgaagccggg
       61 cagcgagacc agctcatcgg gtggggtgag attaacgcgc atggccggca gcacgtcgaa
      121 attcctgcct cccaagcgcg gcatcatcgc cgcctggatc agggaggtct tgcccgcccc
      181 cgaaggggaa tagagcataa tgatgcggtt agcgatcagc aggttggata gcttacgggt
      241 ctcggcctcg cgcccgaaga tcgcttcccc gcgctccagg gcgcgcgggc ccacaaatgg
      301 gttggcagtt gggttgggtt tatcgctcat cggctatctc atatactggt ttaattcttt
      361 caggaaatcc tggctggtgc cccagtacag gtcgatgcgg gcatcctgcc cctctttcag
      421 gtagcgctgg atgtacttgc gagccatctc ggtgtcctcg atcagccctt cgcggggggc
      481 gatctgggct gccacgtggg ggtacaccct cagcccctct gctccaggct ggttcatgat
      541 caggcgggtg atcacgcgga actgccagtc atcgaactgg aagccgacga agagcaggga
      601 cgaatcagcc agggcggcgc gaattgcgtg gggaggcatc aagtcccggt gctcggtcag
      661 tttgatcaag tagtcaaaga tgtcgtcttc ggtatacacc agggaatccg gctctttgaa
      721 gtgtccaaac aggtgataga cgagtggctt ctcgggggtg gggtcgtcgt tgtattccca
      781 cagttgcctg cggttaagca aggaatcttt ccaagggcac aggcggacct gcggctctct
      841 accggcttcc cgcaaggcgt tttgaagcag gtcgctgcgc tcgctggtca aataaatggg
      901 cagccccaga ttggcgagca gcctgtacgg gttgtcatcc gctctggacc actgggcagc
      961 ttcagccaag accagcgcct ggagcaggtg ttctggcttc cagcgctcga gttgcgccag
     1021 ctcggttggg atggcgttgg gaaatccccg ttccagggcc tggcgcaggg ccttttgaaa
     1081 ttgtatgcgc acgtggctct ctttctggaa tacggataca aactgagcga cgcgcggcag
     1141 cttatctcga tcttctttac ataagggata gtgatgcagc tcggaccact gcagggcaat
     1201 ttcccgctca tctccctgga tggagcggac gatttccgga cccagcaaga gggtgcacat
     1261 ctcttcctgg atgtgggtct tcaggctctc ccaggtttcg atatcggagg cttcggaccc
     1321 caagccggga gtgtaccaga tccgcccgct gcgcaggcgc atgaataggg ccggcatcca
     1381 gtgatccgga cggtcgcgca cctgcccgcg cgccaggctg actgccaggt cgaccacccc
     1441 tgtttcttgc aatgcctcga aaaatgcagg cgtgaagcgg gcgttggtca gcatggagaa
     1501 cttaccttgc atagcaagca ccgctggcac gccagcctgc gatagccggg gacccaaagc
     1561 cgaaaaagcc tggcccgcct ccgcttttgc gctgtcacaa gaagccaata ccaccagcat
     1621 gggcatgtca
//
LOCUS       DBNHOH010000929         2466 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1790608, whole genome shotgun sequence.
ACCESSION   DBNHOH010000929 DBNHOH010000000
VERSION     DBNHOH010000929.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1790608"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="AC16V7_16740"
     CDS             complement(<1..754)
                     /locus_tag="AC16V7_16740"
                     /inference="COORDINATES: protein motif:HMM:NF014628.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HIR6258823.1"
                     /translation="MKVTSNTFIPAAQVALLDDEMRRSVDQATTTAYQKRLNLMFEAG
                     REHGEALRQQAAQAKRHALSRLPALLEQAEARLTANGAHVLWALDSDEVNRAVLEIIA
                     RHSVKAVVKSKSMVSEEVGLNEVLESHGIEVVETDLGEYILQLAGEPPSHIVTPVIHK
                     SLEAIRDLFVQKLNMPPTDDARHMVQFTRQILREKFLAADMGITGGNFIIAETGTLCL
                     VTNEGNGRMVTSLPRIHVAIVGIEKVVETLEDF"
     gene            complement(751..1476)
                     /locus_tag="AC16V7_16745"
     CDS             complement(751..1476)
                     /locus_tag="AC16V7_16745"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR6258824.1"
                     /translation="MTCMVDMLYPETGWAVVDVLEYLKIEIDFPPEQTCCGQPGFNAG
                     HRSASREVAIQFLRAFKDAEVIVTPSGSCAAMVHHEYPLLFADDRRWRDEALRAAANT
                     WEFTDYLVEGLGIDDLQIRLPRRHTFAFQDACHGMRMLGLGASARTLLKHTGNAIVEE
                     LEEYDVCCGFGGLFSVKMADISGAMLERKINHIQACSAETIVCGDVSCMTHMNGGLAR
                     LQSAKRVRHIADVLAEGLPGRLT"
     gene            complement(1515..2126)
                     /locus_tag="AC16V7_16750"
     CDS             complement(1515..2126)
                     /locus_tag="AC16V7_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HIR6258825.1"
                     /translation="MIDQRLYEEFRDIGRDIFVTGLTSSHGGNMSVRVGDKIIIKRRG
                     AQFGRLQASDFVETSFQGKDSGIVRASTELIVHRAIYEKTSALAIIHAHPRTAITLSL
                     SRDEIIPIDNEGSYLLRKIPVVSVELASGSKEMADKVSEALRGYKIIMQRGHGCFAIG
                     QTLEEAYHWVSALEEVSDIILEHKLMGEEMIEYRKHSESYKKW"
     gene            complement(2119..>2466)
                     /locus_tag="AC16V7_16755"
     CDS             complement(2119..>2466)
                     /locus_tag="AC16V7_16755"
                     /inference="COORDINATES: protein motif:HMM:NF013481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HIR6258826.1"
                     /translation="KFTNMAVAEEFFARTLSAVTGEHFTADDLMMVGERVWTLERLYN
                     LREGFTKKDDTLPDRMLSEPVSDGPSKGFTVKLAPMLEEYYAFRGWDQNGVPKPEKLK
                     ELSLEFAIEEAHD"
BASE COUNT          528 a          721 c          624 g          593 t
ORIGIN      
        1 caaaatcttc gagcgtctcg acgacttttt cgatcccaac gatagccacg tgaatgcgag
       61 gcaaactggt gaccatgcga ccattgcctt cgttagtcac caggcacaag gttcccgttt
      121 cggcgataat gaagtttccg ccggtaatgc ccatatccgc agcgagaaac ttttcacgca
      181 agatctggcg tgtgaattgc accatatggc gagcgtcgtc cgtggggggc atattcaact
      241 tctgcacgaa caggtcgcgg atggcctcca aagatttgtg aatgactggg gtaacgatgt
      301 ggctgggggg ctcgcctgcg agctggagga tgtactcccc caggtcggtt tcgacaacct
      361 cgataccatg ggattctagc acctcgttca gcccgacctc ttcgctcacc atgctcttgc
      421 tcttcaccac ggctttcact gaatgacggg cgataatttc cagcacagcg cggttcactt
      481 cgtcgctatc cagcgcccac aagacgtgag cgccattggc ggtcaacctg gcttcagcct
      541 gctccaataa tgctggcagg cgactcaagg catgtcgttt cgcctgggcg gcttgctggc
      601 gcagggcttc cccatgttcg cgaccagcct cgaacatcaa attcaaacgc ttctggtagg
      661 ctgtagtcgt tgcctggtcg accgagcggc gcatttcgtc atccagcaaa gccacctgtg
      721 cagccgggat aaaggtgttg gaagtgactt tcatgtgagt ctccctggca gcccctcggc
      781 cagaacatcg gcgatgtgcc gcacgcgttt ggcggactgt aggcgcgcta gcccgccatt
      841 catatgggtc atgcaactta catcgccgca cacgatcgtc tctgcagagc aagcctgtat
      901 gtggttgatt ttccgctcga gcatcgcccc gctgatgtca gccattttga cgctgaatag
      961 ccctccaaag ccgcagcaca catcgtattc ctccagctct tccacaattg cgttgcccgt
     1021 atgttttaac aaggtgcgag ccgaagctcc cagaccgagc atgcgcatac cgtggcaggc
     1081 atcctggaac gcgaaagtat gtcgccgggg caatcgtatt tgaaggtcgt cgatcccaag
     1141 tccttcaacc aggtaatctg tgaattccca ggtattcgca gctgcccgca gcgcttcgtc
     1201 gcgccagcgg cgatcgtccg caaaaaggag gggatactcg tggtgcacca ttgccgcaca
     1261 cgagccagat ggagtgacga tcacttctgc gtctttgaac gccctcagga attggatcgc
     1321 cacctcacgg gaggcagacc tgtgtccggc gttgaacccc ggctgcccgc agcaagtctg
     1381 ctcaggtgga aagtctatct caatcttcaa gtactccaag acgtccacca cagcccagcc
     1441 cgtttctgga tagagcatat ccaccataca tgtcataaat aaagacgccc gcttaccctt
     1501 gggagaatcg cgctttacca ttttttgtaa gattcactgt gcttgcgata ttcaatcatc
     1561 tcctcgccca tcagcttgtg ctccagaatg atatcgctca cttcttctaa tgcggagacc
     1621 caatggtatg cttcttccaa cgtttgccca atcgcgaagc aaccatgccc tcgctgcatg
     1681 atgattttgt agccgcgcag cgcttcagag actttatccg ccatctcttt ggagccggag
     1741 gcgagttcta ccgacaccac cggaatcttg cgcagcaagt aggagccttc gttgtctatg
     1801 ggaatgatct catcccggct tagactgaga gtgatggcgg tgcgtggatg cgcatggata
     1861 attgccagcg ccgatgtttt ttcgtagatg gcgcggtgga cgattaattc ggtgctggcg
     1921 cgcacaatgc cactgtcctt gccctgaaag ctggtttcca caaaatccga ggcctgcaac
     1981 cgcccgaact gcgccccgcg tcgtttgata attatcttgt cccccacccg cacgctcata
     2041 ttgccgccat gtgagctggt taagcctgtc acaaaaatat ctcggccaat atctcggaat
     2101 tcttcatata gtcgttgatc aatcatgtgc ttcctcaatt gcgaactcta aagatagttc
     2161 cttaagcttc tctggcttag gcacgccgtt ttggtcccag cctcggaatg cataatattc
     2221 ttccagcata ggagccagct tgacggtaaa gcctttgctc ggtccatcag agacgggttc
     2281 gctgagcatt cggtcgggca gggtatcatc ttttttcgta aagccctcgc gcaagttgta
     2341 gagacgctcc agcgtccaga cgcgttcacc aaccatcatc aagtcatcag cggtgaaatg
     2401 ttcgccggta acagccgata acgtccgagc gaagaactcc tctgccaccg ccatgtttgt
     2461 aaactt
//
LOCUS       DBNHOH010000930         1454 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1470629, whole genome shotgun sequence.
ACCESSION   DBNHOH010000930 DBNHOH010000000
VERSION     DBNHOH010000930.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1470629"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..926)
                     /locus_tag="AC16V7_16760"
     CDS             complement(120..926)
                     /locus_tag="AC16V7_16760"
                     /inference="COORDINATES: protein motif:HMM:NF016970.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multinuclear nonheme iron-dependent oxidase"
                     /protein_id="HIR6258827.1"
                     /translation="MQVAINYSLEAATLVHEERIHIDRFKTPDWPDMIEEASAYRPVA
                     VHFNLFAGRGDLVSSDWDTIRSLKEQTGTPYINLHLNPEVVDYPDVPLDITKGPLFNE
                     IVHNLILDVQAVVDIFGTEQVIVENVPFRGWRGDTLRLAVDPHIIRKVVEETGCGLLL
                     DLSHARISACYLGVDDQNYITALPVDRLRELHFNGLNWVNGNLKHHLPALRYDWQALS
                     WALKHIRSGEWAPPWLLAFEYGGVGDKYKGRSELTVISEQIPRIFEMISD"
     gene            complement(939..>1454)
                     /gene="mltG"
                     /locus_tag="AC16V7_16765"
     CDS             complement(939..>1454)
                     /gene="mltG"
                     /locus_tag="AC16V7_16765"
                     /inference="COORDINATES: protein motif:HMM:NF014656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR6258828.1"
                     /translation="YQMSRTLTVEGFIRTTLDNFQGHITPEIEQGYINQGLNIYDAVI
                     LASIIEREAVVDEEMPQIASVFLNRLAVGMKLDSDPTAQYALGFNQTQNTWWTNPLSL
                     EHLEVDSPYNTYKYPGLPPGPISNPGLNALQSVAFPATSPYYYFRAACDGSGRHAFSQ
                     TFEEHVGKACP"
BASE COUNT          361 a          351 c          372 g          370 t
ORIGIN      
        1 cgacaagcag ggggatgaat aacattgcaa aaatcttgac gtaccggtgt gcccacatct
       61 atcacctcta aaaattatac ggtcggttag acctgttaga ttttacctga taaatatcat
      121 tagtcggata tcatctcaaa tatcctggga atctgttctg agatcacggt cagttcgctc
      181 cgtcctttgt atttgtctcc aacgccgccg tactcaaaag ccagtagcca ggggggcgcc
      241 cactcaccgc tgcggatatg cttgagcgcc catgacaatg cctgccaatc gtatctcaac
      301 gccggtaggt gatgtttcag attgccgttg acccaattca gaccattaaa gtgtagctca
      361 cgcaggcggt ctactggaag tgctgttatg taattttggt catctacacc caggtaacag
      421 gcggaaatgc gagcatgaga gagatccagc agcaggccac agccagtttc ttcgaccacc
      481 ttgcggataa tgtgtgggtc gactgccagt cgcaacgtgt cgccccgcca gccgcggaag
      541 ggcacatttt caacgataac ctgctccgtc ccaaaaatat ccactactgc ttgaacatcc
      601 aggatgagat tgtgcactat ttcgttgaat agaggccctt tggttatatc tagcgggaca
      661 tcaggataat ccacaacttc tgggttaagg tgtagattga tgtatggggt accggtttgc
      721 tccttcaagg agcggatggt gtcccaatcg ctcgatacca gatcaccgcg cccagcaaag
      781 agattgaagt gtactgcaac cgggcgatag gcgctagcct cctcgatcat atctggccaa
      841 tctggcgttt tgaaacgatc aatgtggatg cgctcttcgt gtactaatgt ggcggcttca
      901 agggagtaat tgatagcgac ttgcatttat ttcgtcattt aggggcacgc cttgccaaca
      961 tgttcctcga aggtttggga aaaggcgtgg cgtccggagc catcacaggc agcccggaag
     1021 taataatatg ggctggttgc aggaaaagct actgattgta atgcattcaa cccgggattg
     1081 gaaatcggtc ccggaggcag accagggtat ttgtaagtgt tgtaaggcga gtccacttct
     1141 aagtgttcta gactgagcgg atttgtccac caagtatttt gggtctggtt gaaacccagg
     1201 gcgtattggg cagtgggatc agaatctagc ttcataccga cagccaaacg attgagaaat
     1261 acagacgcga tctggggcat ctcttcatcc accactgctt cgcgctcgat gatggatgcc
     1321 agtataacag catcgtagat gttcaagccc tggttgatat aaccttgctc aatctctggg
     1381 gtaatatgtc cctggaaatt atccagcgtt gtcctgataa aaccctcaac agtcagcgtg
     1441 cggctcatct ggta
//
LOCUS       DBNHOH010000931         1747 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1661917, whole genome shotgun sequence.
ACCESSION   DBNHOH010000931 DBNHOH010000000
VERSION     DBNHOH010000931.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1661917"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="AC16V7_16770"
     CDS             complement(<1..584)
                     /locus_tag="AC16V7_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258829.1"
                     /translation="MNKWHELNRQKRHNLVRLGIAAAALILIIVYENLEWNWQIGTPR
                     GWWATLDAFLLALLGALAYFSVDRILRMVDERQALKEQLGHAESELNEMRQRQGTLLR
                     ISQMYLDASDEDEVVELALNLSRELLGARGASFVPLDENAQPLEARSLGEVPIADAGS
                     WLEYLASPAVREACDACQNREQLTHVCPLLKGAF"
     gene            complement(850..1311)
                     /gene="bcp"
                     /locus_tag="AC16V7_16775"
     CDS             complement(850..1311)
                     /gene="bcp"
                     /locus_tag="AC16V7_16775"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006625139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HIR6258830.1"
                     /translation="MTLLKGTIAPDFTLLDETSTPRKLTDYRGQPVVLYFYPKDDTPG
                     CTTEACGFRDDYGDYQQAGVTILGVSPDSPKKHTKFKEKYNLPFTLLADTEHAVCELY
                     GVWGRKKFMGREYDGVFRTTYLIGADGVILQVFEKVKPDGHSAEILQALEK"
     gene            complement(1335..>1747)
                     /locus_tag="AC16V7_16780"
     CDS             complement(1335..>1747)
                     /locus_tag="AC16V7_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR6258831.1"
                     /translation="RALQDLARPDVAFVNRQRGAGTRVLLDYHLASLGVAAESVAGYR
                     QEEYTHLAVAAAVASGRADCGLGIAAAAQALDLDFLPLFQERYDLVIPQEYYQSELLA
                     PVLDLLHQAEFRQAIEALPGYDIEPMGAIIAQLD"
BASE COUNT          401 a          531 c          449 g          366 t
ORIGIN      
        1 tggaaggcac ccttgagcaa ggggcacaca tgggtcaact gctcacggtt ctggcatgca
       61 tcgcatgcct cacgcacagc cggcgaggct aggtactcca accagcttcc cgcatctgca
      121 atgggaactt cccccagact cctggcttcc aacggctgtg cattttcatc taatggcacg
      181 aaagacgcac cgcgagcgcc gagcagttcg cgtgacaagt tcagcgccag ttctacgacc
      241 tcgtcttcat cgctggcatc caggtacatc tggctgatac gcaacagcgt gccctgccgc
      301 tggcgcatct cgttcagctc gctttccgcg tgccccagct gctccttgag cgcctggcgc
      361 tcgtccacca tgcgcaagat gcgatctacc gagaaatagg ctaacgctcc caagagcgcc
      421 agcaggaacg catccagcgt ggcccaccag ccgcgcggcg taccaatttg ccagttccat
      481 tccaagtttt catagacgat gatgagaatt aaagcagcgg cagcgatgcc taagcgcacc
      541 agattatgcc gcttttgcct gttcagctca tgccatttat tcacgtgccc tattgtaccc
      601 gaaacgaaag ccctttttgg tgttggagca ggacaatcgc atttgggcga ttccccatca
      661 ctgaggagag aaaatggggt gttttagtgg gggtatgccc ccaccaaaac accccaaaca
      721 ctctttttac gtgattgcgc atgcccctca gggcatatgc aattgccctg ggtgttggag
      781 ctgagcaccg agagcacccc acagcagagc agagccggga ttcacaatgc tgccaccctg
      841 ccgcagcgct tacttctcca aggcctgcag aatttccgcg gaatgcccat cgggtttgac
      901 cttctcgaat acctggagaa taaccccatc cgctccgatc aggtacgtag tgcggaaaac
      961 cccatcatat tcacgaccca taaacttttt gcgcccccaa accccgtaca actcacagac
     1021 tgcatgctcg gtatcagcca gcaaggtgaa gggcaggttg tatttttcct tgaatttagt
     1081 gtgcttcttc ggagaatctg gacttacgcc caaaatcgtt actccagctt gctgataatc
     1141 cccataatca tcgcgaaacc cacatgcctc ggtcgtgcaa ccgggggtat catccttcgg
     1201 ataaaaatag agcaccaccg gctggccgcg ataatctgta agcttgcgcg gcgtgctggt
     1261 ttcatctaat agtgtaaagt cgggggcaat tgttcccttc aagagtgtca ttggtcctcc
     1321 aaagatataa ccgtttaatc taattgtgcg atgatagcac ccatcggctc gatatcatag
     1381 ccaggcagcg cctcgatcgc ctggcggaac tctgcctggt gcagcaaatc cagcacaggg
     1441 gcaagcaact cgctctggta gtattcctgc gggataacca ggtcgtagcg ctcctggaat
     1501 aaaggcaaga aatcgagatc aagcgcctga gccgcagcgg cgatgcccag accacaatcg
     1561 gcgcgacccg atgcaaccgc agcggctacc gcaagatggg tgtactcctc ttgccggtag
     1621 ccagcgacag actctgctgc aaccccgagg gacgcaagat ggtaatccag cagcacccgc
     1681 gtgcccgccc cgcgctggcg gttgacgaag gccacatctg gacgggctag atcttgcagg
     1741 gcgcgga
//
LOCUS       DBNHOH010000932         1267 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_957745, whole genome shotgun sequence.
ACCESSION   DBNHOH010000932 DBNHOH010000000
VERSION     DBNHOH010000932.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_957745"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /gene="hemA"
                     /locus_tag="AC16V7_16785"
     CDS             <1..1046
                     /gene="hemA"
                     /locus_tag="AC16V7_16785"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HIR6258832.1"
                     /translation="RPHTYQLADQQAAEHLLRVAAGLDSLVMGEPQILGQVAEALKQA
                     REMNAAGKLLSRLFYAAIHAGKRARAETAISQNASSIPSLAVRLAERSLNDLPNSQVV
                     VIGAGDMAELAVEAFRKRGVNKILVINRTLERARLLADKWHGEADTFENLTRALQRAD
                     VLIASTSAPHTLVHADQVQQVMQARPHHPLVIIDIAVPRDVDDQVGELPNVQLVDMDT
                     LQAGLEQSLEQRLHEVPSVEDILDDELTQFMDYMRSLDLVPLIVGMRQKAETIRQREL
                     EKTFRRLPELSEAERNSVEIMTQAMVQKILHDPIICLRDEAGGPQAAEIALLVRSLFG
                     LSEAGSHSQQANT"
     gene            1050..>1267
                     /locus_tag="AC16V7_16790"
     CDS             1050..>1267
                     /locus_tag="AC16V7_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HIR6258833.1"
                     /translation="MPPLPLTFATRPSALARWQTQWVIQALQNTWPGLECTDKVITTQ
                     GDRILDRPLPEIGGKGLFTQELEFELHAG"
BASE COUNT          295 a          374 c          349 g          249 t
ORIGIN      
        1 tccgtcccca cacttatcag ttagcggatc agcaagctgc tgagcatcta ttgcgcgtag
       61 ctgctgggct ggactcattg gtgatggggg agccgcaaat acttggacag gtggcagaag
      121 cgttgaagca ggctcgagag atgaacgcgg ctggcaagct cctctcgcgg ctattctatg
      181 ccgccatcca cgctggtaag cgtgcccgag cagagaccgc catcagccag aacgccagct
      241 cgatcccatc cctggcagtc cgcctggcag agcgttctct gaacgattta cccaactctc
      301 aggtggtggt aatcggcgcg ggcgacatgg ctgaactggc tgtggaagcc tttcgtaagc
      361 gcggggtgaa caaaattctg gttatcaatc gcaccctgga gcgcgcacgc ttgcttgccg
      421 ataaatggca tggggaagcg gatacattcg agaacctgac ccgcgccctg caacgcgccg
      481 acgtgctgat cgcctcaacc agcgcacctc acaccctggt gcatgctgat caggttcaac
      541 aggtcatgca agcccgcccg catcatcccc tggtgatcat cgatatcgct gtgccccgcg
      601 atgtagatga ccaggtggga gaattgccca acgtgcaact ggtcgatatg gatacattgc
      661 aggctggcct cgagcaatcc ttggagcaac gcctgcacga agtgccaagc gttgaagaca
      721 tcctggacga tgaactcacc cagtttatgg actacatgcg cagcctggac ctagttccgc
      781 tcattgtagg gatgcgtcaa aaagcggaaa ccatccgcca acgtgagcta gaaaagacct
      841 tcaggcgctt gccagagctt tccgaagctg agcgaaatag cgtagagatc atgacacagg
      901 cgatggtgca aaagatactc cacgacccaa tcatctgctt gcgggatgaa gccggtggtc
      961 ctcaggcagc cgaaattgcc ctattggtac gttcactgtt tggcttgagc gaagccggca
     1021 gccatagtca acaagccaac acctgaacca tgcccccatt acccttaaca tttgctaccc
     1081 gcccatccgc cctcgcccgc tggcaaaccc agtgggtcat ccaagctttg cagaacacct
     1141 ggcctgggct ggagtgcact gacaaggtta taaccaccca aggcgatcgc atcctggatc
     1201 gtccactgcc cgagatcggt ggcaagggtc tattcacgca ggagttagag ttcgaactgc
     1261 acgcagg
//
LOCUS       DBNHOH010000933         1250 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_957779, whole genome shotgun sequence.
ACCESSION   DBNHOH010000933 DBNHOH010000000
VERSION     DBNHOH010000933.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_957779"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="AC16V7_16795"
     CDS             complement(<1..659)
                     /locus_tag="AC16V7_16795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258834.1"
                     /translation="MTLPTATNDWTLRTEILEIAHRWPQVFIAFLLGSLLGWGAALIF
                     PTSYRAQTEIYVAYNADAIYRNPDDYKNWQFEELDAYIVSNDVLSETMQRLRQQDPYW
                     QDIKIKDVKPNLRTFWRSAGRWRLVADWSDQQHATQLSQTWTEVILEKTSQAATHAQA
                     VLDLEAQIAAHSKDEVNVRLQFTQLTQLKNALETWRQAQDPASNQPLDTLARWRLQFL
                     AA"
     gene            complement(637..>1250)
                     /locus_tag="AC16V7_16800"
     CDS             complement(637..>1250)
                     /locus_tag="AC16V7_16800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR6258835.1"
                     /translation="GVDPERFTPQPTPPEPVVILLAGRMLWDKGVGVLVEAARLLRRR
                     TTARVVLVGTPDPGNPATIDESILAQWNQEGVIEWWGWHADMPSMYNQSHVVALPTAY
                     GEGVPTVLLEAAACAKPLVASDIPGCRDVIQEGVNGILVPPNDSQALADALEHLVRNP
                     QLRSRMGAAGRQLVLEKFTYEQINTATLEVYRHVLNHDFTNRN"
BASE COUNT          249 a          348 c          366 g          287 t
ORIGIN      
        1 gctgccagga actgcagccg ccagcgggct aaggtatcta gcggctggtt gctggcaggg
       61 tcttgcgcct gccgccaggt ttccaaggcg ttcttcaact gggtgagctg ggtaaattgc
      121 agcctgacgt ttacctcatc cttggagtgg gccgcgattt gagcttccag atcgagcact
      181 gcctgtgcgt gggtggctgc ctggctggtc ttctccagga tgacctctgt ccaggtctgg
      241 gatagttggg tggcgtgctg ctggtcgctc caatcagcta ccaagcgcca tcttccggcg
      301 ctgcgccaga aggtgcgcag attgggcttc acatccttga ttttgatgtc ctgccagtag
      361 gggtcctgct gcctcagccg ctgcatggtc tcgctgagca catcgttgga gacgatatag
      421 gcgtctagct cttcgaactg ccaattcttg taatcatcgg ggttgcggta gatggcgtct
      481 gcgttatagg ctacgtaaat ctctgtctgg gcgcgatatg aggtggggaa gatcaacgca
      541 gcgccccaac ccagcaggct gcccaggaga aatgcaatga acacctgcgg ccagcggtga
      601 gcgatctcaa gtatctcagt gcggagggtc caatcgttag ttgcggttgg taaagtcatg
      661 attgagaacg tgtcgataca cttctagtgt cgctgtgttg atttgctcgt aggtaaattt
      721 ttccaaaact agctgacgac cagcggctcc cattctacta cgaagctgcg ggttgcgtac
      781 caggtgttcc aacgcatccg ccaacgcctg agaatcattg ggaggcacca gaattccgtt
      841 cacgccttcc tggatgacat cccggcagcc aggaatatcg ctggcaacca ggggttttgc
      901 acaagctgcg gcctctaata ataccgtggg cacgccttcg ccgtaagcgg tcggcaaggc
      961 cactacgtga ctctgattgt acatcgaagg catgtccgcg tgccagcccc accattcaat
     1021 aactccctct tgattccatt gagcaaggat ggattcgtct atcgtggctg gatttccagg
     1081 atccggtgta cccacaagga ccacgcgggc tgttgtgcga cggcgcagaa gacgagccgc
     1141 ctcaaccagc acgccaactc ctttatccca cagcatacga cccgccagca agatcaccac
     1201 tggctctggg ggagtgggtt ggggggtgaa gcgctcaggg tcaaccccca
//
LOCUS       DBNHOH010000934         2322 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_893178, whole genome shotgun sequence.
ACCESSION   DBNHOH010000934 DBNHOH010000000
VERSION     DBNHOH010000934.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_893178"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            337..990
                     /locus_tag="AC16V7_16805"
     CDS             337..990
                     /locus_tag="AC16V7_16805"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6258836.1"
                     /translation="MVRVIWIEGKRAEGPSFVPNMRKKGYSVEVVSTGTEALERLVEI
                     DPDLAVVNAASMRTSGKRICRSLREKMNGLPIVLINSPEQPALGDPSANVVLTLPFTF
                     RKLSNRIVPLLPGNGGNMLHVGPIRLDMGLKRVRCQGREARLTPRLVRILRILMQHPG
                     EVVERAVLFRHVWDTEYTEDTRTLDVHISWLRQAIEEDPRKPQFLKTIRGVGYRLDI"
     gene            complement(1096..1437)
                     /locus_tag="AC16V7_16810"
     CDS             complement(1096..1437)
                     /locus_tag="AC16V7_16810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258837.1"
                     /translation="MPSELETALKNTATSVAKYVQDASELQVETRYVDVSSDGTPDFD
                     QARPVARTVIRLDGDSESVVPMRKGTGGALEVDATLFEVHQQNVAAAVDYRARILNAL
                     LSALPSRARTP"
     gene            complement(1451..2245)
                     /locus_tag="AC16V7_16815"
     CDS             complement(1451..2245)
                     /locus_tag="AC16V7_16815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258838.1"
                     /translation="MLNKQTMQNEAGFPPIFQNWQTAPVQSGDEISFDVATAPGEPAS
                     RWSLELPAKPEEAEAALAQAETQLRATQALLDEAPDRLDAFVSRLQSGTDAHFSIESF
                     AVEGVPGQPAEADLLPWIEYFEPGQISFDGQGITQGQAKEAFDQLRQMIDGLLGQILY
                     LAQVETRQGGALLARSLVNWKGDLDTSWGEAISPEQHDLHQRSLALALASRIAMLRVI
                     MTTTQGAAKIAALIAAPGGALLALPAAWKYVSKILSEIEKYKTSSA"
BASE COUNT          493 a          593 c          669 g          567 t
ORIGIN      
        1 cagggttgca actatattta tcaaaggcca gggcaattgc atatgcccgg aagggcatgc
       61 gcaatcccgc aaaaagagtg tttggggggt tttggtgggg gtaggccccc accaaaaccc
      121 cccattttct ctcctcagtg gtggggaatc gcccaaatgc gattgccctg tatcaaaggc
      181 tttttaggat ttgtcttgac cttggagatt ctcagttatc cttaaaaaag cacatcggtt
      241 ctgattgtca gtttttaaga ttgcgagtat aatcaattta acattttttt taacattggg
      301 gtttaaaaat tagcgattat accgataggg aagagaatgg ttcgggttat ttggatcgag
      361 ggcaagcggg ctgaaggccc gtcttttgtc ccaaatatga gaaaaaaagg ctattccgtg
      421 gaggttgttt ccacgggaac tgaggcgttg gaacgattgg tggagatcga cccagacctt
      481 gcggttgtta acgcggcctc gatgcgcacc agcggaaaaa ggatttgccg atctctgcga
      541 gaaaaaatga atggtctccc gattgtgctg atcaacagcc cagagcagcc cgccctgggt
      601 gacccgagcg ccaatgtggt cctcaccctt ccgttcacct tccgcaagct gtctaatcgc
      661 attgtgcctc tgttgcctgg aaatggcggg aatatgctcc atgtggggcc tatccgcctg
      721 gatatgggac taaaacgcgt gcgatgtcag ggcagagaag cccgtctcac cccccgcctg
      781 gtacggatcc tgcgcattct gatgcagcat cccggcgagg tggtggagcg agctgtcttg
      841 ttccgccacg tatgggatac ggagtacacc gaggatacgc gcactctgga cgtgcacatc
      901 agctggctgc gccaggcgat cgaagaagac cctcgcaaac ctcagttctt gaaaaccatc
      961 cggggggttg ggtaccggct ggatatttaa tcagtttttg gtgcgcaacg gcagtagtat
     1021 aaccttatca accgcagtgt aactgcggtt gatgttgttt ttccatattg tattattaca
     1081 gcagctgaga tttgattatg gcgtgcgcgc ccgactgggc agcgcactga gcagggcatt
     1141 gaggatgcgc gcacgataat cgactgcagc ggcgacgttt tgctggtgca cctcgaacag
     1201 ggtagcgtcc acctctaatg ctccacctgt tcccttacgc atggggacga ctgactcgct
     1261 gtcgccatcc agccggatga ccgtgcgcgc caccgggcga gcctgatcga agtcaggggt
     1321 gccatcgctg ctgacatcga cataacgggt ctctacctgt aattcagatg catcctgcac
     1381 gtatttggct acgctggtag ctgtgttctt aagggcggtt tcaagttcgc tgggcatttt
     1441 tactcctaga ttatgcggat gaagttttat atttttcgat ttctgataat atcttgctca
     1501 cgtatttcca ggcggctggc agggccagca aggcgccccc gggcgcggcg atcagggccg
     1561 cgattttagc agctccttgg gtggtggtca taatgactcg cagcatggcg atgcgcgagg
     1621 ccagagccag cgctaatgag cgctggtgca agtcgtgctg ttcgggagag atggcctcgc
     1681 cccaggaagt gtccaggtcg cccttccagt tcaccaggga gcgagccagc agagccccac
     1741 cctggcgcgt ctcaacctgc gccaggtaga gtatctgtcc aaggaggcca tctatcatct
     1801 ggcggagctg atcgaaagcc tccttcgcct gaccttgggt tattccctgc ccgtcaaatg
     1861 agatttgccc gggctcgaag tactcaatcc agggcaataa gtcggcttcg gcgggttgcc
     1921 ctggcacgcc ttcgacagcg aaggattcga tgctgaagtg cgcatctgtg ccagactgca
     1981 agcgcgagac gaacgcatcc agacgatcgg gggcttcatc taggagcgcc tgagttgccc
     2041 gcagctgcgt ctcagcttga gccagtgctg cctcagcttc ttccggtttg gcaggcagct
     2101 ccaggctcca ccgcgaggcc ggctcgccag gagcggttgc gacgtcaaag gatatctcgt
     2161 cgccactttg tactggggcg gtctgccagt tctggaaaat gggtggaaaa cccgcttcgt
     2221 tctgcatggt ttgtttattt aacacaaagg gcacgaaggg tgtagaaaaa acatttaaaa
     2281 ctcttggtgt tcctttgtgt taaggatcgt tttagggttt ta
//
LOCUS       DBNHOH010000935         2562 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1790948, whole genome shotgun sequence.
ACCESSION   DBNHOH010000935 DBNHOH010000000
VERSION     DBNHOH010000935.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1790948"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="AC16V7_16820"
     CDS             <1..389
                     /locus_tag="AC16V7_16820"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIR6258839.1"
                     /translation="RAFDGFDPQRVAQYGDEKVAALLADPGIVRNRLKISAAIRNARS
                     FLEIQSECGSFDAYIWQFVGGKPKVNAWRNLTVIPASTPESDAMSKDLARRGFKFVGS
                     TICYAFMQAVGMVNDHTVDCYRYNEI"
     gene            658..1614
                     /locus_tag="AC16V7_16825"
     CDS             658..1614
                     /locus_tag="AC16V7_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6258840.1"
                     /translation="MRGSKKSEPKQLVALEGVNLQVRRGELFGLLGPNGAGKTTLIKI
                     LTTLLSPSSGWARVAGFDVVKEPQNVRLHINMVSGGESSGYGLLTVRENLWMFSQFYG
                     MPSAQANQRIKELLQVVGLSDRINTKSSDLSTGLRQKMNIVRGFLTDPDVLFLDEPTL
                     GLDVGASRDVRRFIRRWVDEDPSRTILITTHYMAEADELCDRVAIINKGQVLACDTPA
                     TLKHRLQGEAIIRMEVSPLNGLGVDIFESLPGVRKVVHRPQDGFSVLEFILEQDDALG
                     AVVNTMTSANIHILNLHKREPSLEDVFVDLVGRSMEQVEKGE"
     gene            1611..2441
                     /locus_tag="AC16V7_16830"
     CDS             1611..2441
                     /locus_tag="AC16V7_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6258841.1"
                     /translation="MRVKKNSGWILFLQAVIARAYPRVIGQQREKSWMFFEIFLPMLS
                     VAAYVFVYRAIGAPEEYVGFVVLGGAMMAFWLNVLWNMSSQLYWEKEQGNLALYIIAP
                     NSMMAILLGMAVGGLFATLLRAVVIVGLGSWMFQVRYEIASFPLLCAVFILSMVALYG
                     LGMMSASIFLLLSREAWHLVSLAQEPVYLASGFYFPIKNLNFWVAAAASIIPLTLGLD
                     AMRQLAFPSGEALGFLSVQVELGVLLVLCFLFLLGARWLLNYIEKLAIREGRLTESRR
                     "
BASE COUNT          522 a          612 c          760 g          668 t
ORIGIN      
        1 gacgcgcctt tgatggattt gatccccagc gggtggcgca gtatggcgat gagaaggtcg
       61 ctgccttgct tgcagacccc ggcattgtgc gcaaccggct gaagatctct gcagcaatcc
      121 ggaacgcgcg ctctttcctg gagattcaaa gtgagtgtgg cagtttcgat gcctatatct
      181 ggcagttcgt tggaggaaaa cccaaggtca acgcctggcg aaacctgaca gtgattccag
      241 ccagtacgcc cgagtcagac gcaatgagta aagacctggc gcgtcgcggc ttcaagttcg
      301 tcggttcgac catctgctat gctttcatgc aagccgtagg tatggtcaac gatcataccg
      361 tggattgcta tcgatataat gagatttaaa ttgaagggaa actatttgtt gtcaaaaagg
      421 gggattgaga gggggcctgt gttagaatcg gcgagagccg ggtacgccgc ccccggctct
      481 ctctaaggag gagaagaaga gaaatcgccc ttcaattgtt atattaccag aggcgacgaa
      541 tctctgcttg acgattaccc tacgattacc ctacgattgc ccatcaattt tgagggtttt
      601 ggcaatatta ctatggattt tgcaattgaa accgaacatt tgagcaggat ttacaagttg
      661 cgggggagta aaaagagcga gcccaagcaa ctagtcgcct tggagggcgt caatctgcag
      721 gtgcgccgtg gcgagttgtt tggcctactc ggtccaaatg gcgcaggaaa gaccaccctg
      781 atcaagatcc tcaccacgct gctctcgccc tcctctggct gggcacgcgt agcaggtttc
      841 gatgttgtca aagaacccca aaatgtacgc ctacacatca atatggtctc tgggggcgag
      901 tcgtctggtt atggcctgct cacggtgcgg gagaatctgt ggatgttttc ccagttctac
      961 ggcatgccct cagcccaggc gaaccaacgt ataaaagagt tactgcaggt ggtggggtta
     1021 tctgatcgga tcaataccaa gtcatctgat ctctccaccg gcttgcggca gaagatgaac
     1081 atcgtgcgcg gttttctcac cgacccggac gtgctcttcc tagacgagcc cactctgggg
     1141 ttagatgttg gcgcttcgcg ggatgtgcgt cgctttatcc gtagatgggt ggacgaagat
     1201 ccctcacgta caatcttgat cactactcac tatatggcgg aggccgacga gctgtgtgac
     1261 cgggtggcga tcatcaacaa ggggcaagtt ctggcctgcg atacacccgc aaccttgaag
     1321 caccgtttgc aaggcgaggc gattattcgc atggaggtca gcccgctcaa tggattggga
     1381 gtggatatct tcgaatctct accaggagta cgcaaggttg tccaccggcc ccaggatggt
     1441 ttttcggtct tggagttcat cctcgaacag gatgatgcct tgggtgcagt cgtcaacacc
     1501 atgacgtctg ctaatattca tattctcaac ctgcataagc gcgaaccttc actagaagat
     1561 gtctttgtcg atctggttgg gcgtagtatg gagcaggtgg agaaaggcga atgagggtca
     1621 agaaaaacag tggctggatt ttgtttctgc aagctgtgat cgcgcgcgcc tacccgcgag
     1681 tgattgggca acagcgggag aagtcctgga tgttctttga gatattcttg cctatgctct
     1741 ctgtggcggc ttatgtgttt gtctaccgcg ccatcggcgc gccggaggaa tacgttggct
     1801 tcgtcgtctt gggtggggcg atgatggctt tctggttgaa cgttttgtgg aacatgtcca
     1861 gccagttgta ttgggagaag gagcagggta acctggcgct gtacatcatt gcgcccaact
     1921 cgatgatggc catcctgctg ggtatggcgg tgggcggtct atttgccacc ctgctgcgtg
     1981 cggtggtgat tgtggggttg gggagctgga tgttccaggt tcgttatgag atcgcaagct
     2041 ttccgctttt gtgcgccgtg tttatattat caatggtagc cctgtacggt ctggggatga
     2101 tgagtgcctc gatctttctg ttattgagcc gggaggcctg gcatctggta agtttggcgc
     2161 aggagccggt ctatttagct tccgggtttt atttccccat caaaaatctc aatttttggg
     2221 tggctgcggc tgcttctatc atccccctca ccctggggct ggatgccatg cggcagctgg
     2281 ctttcccatc tggtgaagcc ttgggtttcc tctctgtgca ggttgagctg ggcgtgctgc
     2341 tggtgttatg ctttcttttt ctgctgggtg cgcgctggct tttaaattat atcgagaagc
     2401 ttgccattcg cgaggggcgg ctgacagaga gccgacggta aatgatgatg tggcgctcgt
     2461 ttcgcatggc ttcctggttg ggctggcaga tcgaatcgaa ctgggcagat ccgttcctgt
     2521 tcgcagtata ttcgttcgcc aaacccttgg caggggcggc ta
//
LOCUS       DBNHOH010000936         6956 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_636905, whole genome shotgun sequence.
ACCESSION   DBNHOH010000936 DBNHOH010000000
VERSION     DBNHOH010000936.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6956)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6956)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6956
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_636905"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..832
                     /locus_tag="AC16V7_16835"
     CDS             74..832
                     /locus_tag="AC16V7_16835"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR6258842.1"
                     /translation="MIDHGEGEFLKRRSLKEEIFQILHQKIVAGKIAPGEWLRQEEIS
                     NQLGVSQTPVREALDLLVSAGLAERIPYRGVRVIQLTTEEIVDAYIMRLILESTAGRL
                     AAHNITPEQIRDINEIIEQTRGLVTLEDMSTQRQLNKKFHMAIVFASGNTLLTRLYEA
                     ASNLFPDWMLYEYMFRHPELLQYSLRREYQEHKAIADAITQHDADQAAQKIVEHIRNL
                     GNEFESFLNIPGEFIREKEQQAAPVWMAKIISSA"
     gene            935..1585
                     /locus_tag="AC16V7_16840"
     CDS             935..1585
                     /locus_tag="AC16V7_16840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HIR6258843.1"
                     /translation="MQTEDLFKAMSQSIIEGESETAAELARQAIELGIDPLEAINAGF
                     VHGINHVGDEFSCGNMFLPELVMAGEAMKTAISVLEPEMSKLGTERQVYGKVVLATVQ
                     GDIHDIGKTLVGTMLSAYGFQVYDMGVDVPITAIVEKAREVDADIVAVSALLTTTMVR
                     QRDVMEALDDMGLRAKVKVMVGGAPVTQEWVQQIGADGYSEDAIGAVSVAKKLMGV"
     gene            1595..3046
                     /locus_tag="AC16V7_16845"
     CDS             1595..3046
                     /locus_tag="AC16V7_16845"
                     /inference="COORDINATES: protein motif:HMM:NF018012.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR6258844.1"
                     /translation="MTQRIKSITNPKLKLEILTPEEVRRIHTTTLDIIENVGVRFPSQ
                     KALDIWDAHGAQVNRDSSIVKVPGHIIEEALKKAPSAYPLAARNPSQDLPLDGNHVFL
                     GTDGCGVEVLDIQTGKRRTSCWQDVADIARLGDALPELAFHWVAVSAQDCPPATRGLQ
                     ELRAVWENSTKHVQTESIYHEREAIASIEMAATIAGGREALRVRPVLSLMQCTAPPLG
                     HDGGSLEAALIAAEAGIPTGFMTMAACLTTGPATLAGTLAVGNAEVISATALLQLAYP
                     GAPVFYAAAQTASDPRSGAYTGGGPEDFLFGAATNLLSDFYNIPLSMGSFATGAKEPN
                     WQAGIDNSLSTFMACVVMSDMLLGVGLLHGSRIWSYEQMLMDCEIFSIVHKMMQGIVV
                     DDETLALEAISAVGPGGNFLTQKHSLRHMRELFIPQFMDRRPYNVWEEKGDGSRQWAL
                     EKARRILESHQPEPLDPQLSAELQRIIATVDKG"
     gene            3053..4528
                     /locus_tag="AC16V7_16850"
     CDS             3053..4528
                     /locus_tag="AC16V7_16850"
                     /inference="COORDINATES: protein motif:HMM:NF018012.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR6258845.1"
                     /translation="MQPKLTLLSNDLIQRILDEAYQLLMNPGIKVQSPEARQLLAEAH
                     AQVDETNEIAKIPRQVVEKALESVPRQFSLYDRDGQPTVNYGGDAVHFDPGSSGVHVL
                     DPETLEHKPSYSADLVRIIKIAEMLPQYDAQSTAVVCNEIPKAIGDLYRLYLVLLYSK
                     KPIVTGAFSVETLQAMIDMLAIFAGGRAGLEKKPQAVFDVCPSPPLIWSEFGAQNLID
                     LGRAFVPVQIVSMPLAGAAAPVTLLGSVVQHAAECLSGITIHQLAQPGAPIIWGGAPA
                     IFDMRKGTTPMGAVETAMIDAAYAEVGKSLGLPTHTYLGASDAKLVDAQAGLESGITA
                     LIGALAGINMISGAGMLDFLACVSPEKLVVDAEAIAMVKRLRQGMQVHTDTLALAMFE
                     GINFKGDFLKQRITRELFSKEQYIPSKVIDRDSIRGWQQDGSLDTFARAKLRTQALLE
                     EYQPPQIPRAQAEELHSMVAALARRAGMDRLPQIGEEAHRR"
     gene            4626..5693
                     /locus_tag="AC16V7_16855"
     CDS             4626..5693
                     /locus_tag="AC16V7_16855"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6258846.1"
                     /translation="MTTRKIFQITVVLLVAASMVLAACAPQAPKPSGPLVMLDWAGYE
                     IPEFWGEFATKYPDVKVEFSFLTESAEVYSKLQSGFEADLVHPCSNFWKLLVDENLVQ
                     PIDTSKLSRWNSVIESLAKEGEFNGKQYWIPWDWGFESILVRKDLVEQVPTSWADIWD
                     PKYAGQIAINDSGEAAHIWASLALGLDPWNQTDEQYAQVRQKLLELAPSVKAYWTGQT
                     DLDQMLASGDVSLGVGAWNASYVSLLGEGLDVDYIAPKEGRTGYLCGFGIPTSAKNVD
                     LAHEMIDAYLAPESMVYLANEYGYGIVNEDAIPQVDPEMAELLGLEDPNVISQTVFYK
                     SITSEQRELWTNTWSEVKTSQ"
     gene            5780..6631
                     /locus_tag="AC16V7_16860"
     CDS             5780..6631
                     /locus_tag="AC16V7_16860"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6258847.1"
                     /translation="MENRSRAAYLVGPPAVYLLFLFILPLGIMAIFSFRAGTIGAASQ
                     QFTLDNYIDFIQNATYHRLLWRSALIAFTISAVSVLFAYPLAYFLVFIAGERRITFLT
                     LIIVPTWTSYLLRIFAWKLILGSQGLLNTLLLWLGLIEQASSILLYSRTAVVITLIYV
                     WVPFVALPIFAALERIDRNLLEAAADLGCRPWEAFLRVTLPLSMPGVVAGFMFALIPT
                     LGEFVTPLLVGGGQGAMYGNLIQDQFVRALNWPMGSVMSLAMLVMVLLIIFAFLRGGS
                     AAKLLDI"
     gene            6632..>6956
                     /locus_tag="AC16V7_16865"
     CDS             6632..>6956
                     /locus_tag="AC16V7_16865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6258848.1"
                     /translation="MKRQHSFSLFTLYYWLVTAVLFLPIILLIIFSFNNSITLVFPLK
                     GFTLKWYQQLIQNYELIRSVRTSLLLGIGTSLVATALGAMGAIAMVRFNFPGKSLFIS
                     VAAMPL"
BASE COUNT         1594 a         1989 c         1891 g         1482 t
ORIGIN      
        1 atgcaggaac atttctcgac caccccatac cctccattgg ctctcccctt catactaata
       61 aggcaggtac ggcatgatcg atcatggaga aggcgaattt ctcaagcgca gaagcctgaa
      121 ggaagagatt ttccaaatcc ttcatcaaaa aatcgtcgcg ggaaaaattg ccccaggcga
      181 gtggctgcgc caggaggaaa tctccaacca gcttggtgtc agccaaaccc ctgtgcgcga
      241 agcgcttgac ctgcttgtct cagccggtct cgcggagcgc atcccctatc gcggggtgcg
      301 tgtgatccag ctcaccacag aagaaatcgt agacgcctac ataatgcgcc tgattctgga
      361 gagcaccgcc gggagattgg ctgcccataa catcactccc gaacaaatac gagatattaa
      421 tgaaatcatc gagcagaccc gggggctggt aacactagaa gatatgtcaa cccagcgcca
      481 attgaataaa aaattccaca tggctatcgt cttcgccagt gggaatacgc tattgaccag
      541 attgtacgaa gcggcctcaa atcttttccc agactggatg ctctacgagt acatgttccg
      601 ccacccggag ctccttcaat acagcctgag gcgcgagtac caggagcaca aagccattgc
      661 tgatgcaatc acccaacacg acgcagacca ggccgcccaa aaaatcgtgg agcatatccg
      721 taatctaggg aacgaatttg agtcgttttt aaacattccg ggcgaattta tccgggagaa
      781 agaacagcaa gccgccccgg tttggatggc gaagataatc tcatccgcat agcaagtcat
      841 ccagaaggga ggatggggcc ttacactgaa ttgcctagaa atttaaatca tcaaggtttc
      901 acaaaactta accctcattg cacaggagct aattatgcaa accgaagatt tattcaaagc
      961 catgtcccag agtattattg agggcgagtc agaaaccgcc gctgaattag cccggcaggc
     1021 catagaattg ggtatagatc ccctggaagc catcaacgca ggcttcgttc acgggatcaa
     1081 tcatgtcggc gacgagttta gctgcggcaa tatgttcttg cccgagctgg tgatggccgg
     1141 cgaggcgatg aagaccgcca tcagcgtgct agagccggaa atgtccaagc taggtaccga
     1201 gcggcaggtc tatggaaagg tagtactggc tacggtacag ggcgatattc acgatatcgg
     1261 caagacactg gtgggcacca tgttgtctgc ttacggcttt caggtgtatg atatgggtgt
     1321 cgacgttccc atcactgcca tcgttgaaaa agcccgtgaa gtggacgcgg atatcgttgc
     1381 tgtgagcgcc ctgctgacca ccaccatggt gcgtcagagg gatgtcatgg aagctttgga
     1441 cgacatgggg ttgcgagcga aggtcaaggt tatggtcggc ggagcgccgg tcacccaaga
     1501 atgggtgcag caaatcggcg cagatggtta cagtgaagac gccatcgggg cggtgagcgt
     1561 cgcaaagaag ctaatgggcg tgtagaggca ttcgatgacc cagcggataa aatctatcac
     1621 taatccaaaa ctcaaactgg aaatcctcac gccagaagaa gtgcgccgca tccatacaac
     1681 cacattagat atcatcgaga acgtgggtgt gcgtttccca tctcaaaaag cgctagatat
     1741 ctgggatgcg cacggtgccc aggttaaccg cgattccagt atcgtcaaag tccccgggca
     1801 tatcatcgaa gaggcattga aaaaagcccc ttcagcctac cccttggcag cccgaaatcc
     1861 ctcccaagac ctgcccttag acggaaatca tgtcttctta ggtacagatg gctgcggtgt
     1921 ggaggtgcta gacatccaaa ccggcaagcg ccgcacatcc tgctggcaag atgtagccga
     1981 tattgcccgc ctgggtgacg ccctgccgga gctggctttt cactgggtgg ccgtctccgc
     2041 ccaagactgc cctccggcaa ctcgcggctt gcaagaattg cgtgctgtct gggagaattc
     2101 caccaagcac gtgcagacag agtcaattta ccatgagcga gaagcgatcg cttccatcga
     2161 gatggctgca actatcgctg ggggcagaga agcgctgcgg gtgcgcccgg tgctctctct
     2221 catgcagtgt accgcccctc cgctagggca tgatggcggc agcctggaag ccgcgctgat
     2281 cgctgcagaa gctggcatcc ccaccggttt tatgaccatg gcagcctgcc tgaccacagg
     2341 tccggcgact ttagctggca cgctggcggt tggcaatgcc gaggtgatca gcgccacagc
     2401 cttgctgcaa ctggcttatc cgggagcccc ggttttctat gccgccgcgc aaactgcctc
     2461 cgatccgcgc tctggcgcct acaccggcgg agggccggaa gatttccttt tcggcgctgc
     2521 caccaacctg ctctccgatt tctacaatat cccgctatcg atgggctcct tcgccaccgg
     2581 cgccaaggag cctaactggc aagccggcat cgataacagc ctgtccactt tcatggcctg
     2641 cgtagtcatg tcagatatgt tgcttggagt agggctgctg catggaagcc gtatctggtc
     2701 gtatgagcag atgctgatgg actgcgagat tttctccatt gtgcacaaga tgatgcaagg
     2761 cattgtcgta gacgacgaga ccctggcgct ggaagccatt agcgcggtgg ggccaggcgg
     2821 gaatttcctg acccaaaagc atagcctccg gcacatgcgc gagctattta tcccccagtt
     2881 catggatcgc cgaccctaca atgtgtggga ggaaaaaggc gacggatcgc gccagtgggc
     2941 tctggagaaa gcacgccgca tcctggaaag ccaccaaccc gagcctctcg atccgcagct
     3001 atcagcagag ctgcaacgca tcattgctac ggtggataaa gggtaaatca acatgcagcc
     3061 caagcttacc ctcctctcca acgatctaat ccagcgcata ctggacgaag cctaccagct
     3121 cctgatgaac cctggtatca aggttcaatc ccccgaggcc cggcagctgc tggctgaggc
     3181 gcatgcccag gtggacgaaa ccaacgagat cgccaagatc ccccgccagg tggtcgagaa
     3241 ggccctggaa agtgtgccac gccagttcag cctctacgac cgggatggcc agcccacggt
     3301 aaactacggc ggagatgccg tgcacttcga tcctggttcc tctggtgttc acgtcctcga
     3361 ccccgagacg ctggaacaca aaccttcgta ttccgccgat ctggttcgga tcatcaaaat
     3421 tgcagagatg ctcccacagt acgacgccca gtctactgca gtggtgtgca acgaaatccc
     3481 caaggctatt ggagacctat accgtttgta cctggtgcta ctgtattcca agaaacctat
     3541 tgttaccggt gccttctctg ttgaaacgct ccaggcgatg attgacatgc tggcgatctt
     3601 tgccggcggg cgggctgggt tagaaaagaa accgcaagcg gtcttcgatg tgtgcccgtc
     3661 acccccgctt atctggagcg aattcggcgc acagaacctg attgacctgg ggcgcgcttt
     3721 tgtacctgtc cagatcgtct ccatgccact agccggggca gccgcgcctg tcaccttgct
     3781 aggctcggtt gtgcaacatg ccgccgaatg cctgagtggt atcaccatcc accagcttgc
     3841 ccagccgggt gcgccgatca tctggggtgg cgcgccagcc atcttcgaca tgcgcaaggg
     3901 caccactccc atgggagctg tcgagactgc catgatcgac gcggcgtacg ccgaggtggg
     3961 caagtccttg ggcttgccaa cacacactta cctgggtgct agcgacgcca aactggtcga
     4021 cgcccaggct ggactggaaa gcgggatcac tgccctgatc ggcgctttgg caggtatcaa
     4081 catgatctcc ggggcaggta tgctggattt tctagcctgc gtgagccccg aaaaactggt
     4141 ggtggacgcg gaggctatcg ccatggtcaa gcgcctgcgc cagggtatgc aggtgcacac
     4201 cgatacgctg gccctggcga tgtttgaggg aatcaacttc aaaggcgatt tcctcaaaca
     4261 gcgtatcacc cgcgagctat tctctaagga acagtatatc ccctccaaag tgatcgaccg
     4321 cgattctatc cgtggctggc agcaggatgg cagcctggat acctttgccc gggcgaaact
     4381 ccgcacccaa gccctgttag aggagtacca acccccacaa atcccgcgcg cgcaagcaga
     4441 ggagctccac agcatggtcg cagcgctcgc acggagggcg gggatggaca ggcttcccca
     4501 aattggcgaa gaagcccata ggcgataaaa cgaacaactg aaccaaaagg aggagactgg
     4561 gagaagcaaa ccgcagaaca attgtccatt catcgtttcg actcatctag tgaaggagag
     4621 ttcaaatgac cacccgaaag atattccaga tcaccgtagt gctactggta gccgcctcca
     4681 tggtactagc tgcctgcgcc ccgcaagccc ctaaacccag cggcccgctg gtcatgctgg
     4741 actgggcggg atatgaaatc cccgagtttt ggggggaatt cgccaccaaa tacccggatg
     4801 taaaggtgga gttcagcttc ctgactgaaa gcgctgaagt ctattccaaa ctgcaaagcg
     4861 gcttcgaggc cgacctggtt cacccgtgct ccaacttctg gaaactgctg gtggacgaga
     4921 acctggtgca gcccattgac acctccaagc tgagccgctg gaacagcgta atcgaatcac
     4981 tggccaagga aggggagttc aacggtaagc aatactggat tccgtgggac tggggatttg
     5041 aatccatcct ggtgcgcaag gacctggttg agcaagtacc aacttcctgg gcagacatct
     5101 gggatcccaa atacgccggt caaatcgcca tcaacgattc gggggaagca gcccatatct
     5161 gggcttcgct cgctttagga ttggatccct ggaaccagac cgatgagcaa tacgcccagg
     5221 tcaggcaaaa actattagag ctggctccct ccgtgaaggc ttactggacc gggcaaacgg
     5281 acctggacca gatgctggcc tccggcgacg tctcgctagg ggtgggcgca tggaacgcct
     5341 cgtacgtcag cctacttggt gagggcctgg atgtagatta catcgcaccc aaagagggta
     5401 gaaccggtta cctgtgcggg tttggcatcc caacctctgc aaagaacgtt gacctggctc
     5461 atgagatgat tgacgcttac ctggcgccgg aatcgatggt ttacctggcc aatgaatatg
     5521 gctatggtat cgtcaatgaa gacgccattc cccaggtcga cccagagatg gctgagctgc
     5581 tcggtctgga agatcccaat gtgatctcgc agaccgtgtt ctacaagtcc atcacgtctg
     5641 aacagcgcga gctgtggacc aacacctggt ccgaagtgaa gacctcgcag tagcgttctg
     5701 tacctgaaca gcctgggcct ctccagttag gcctcagcga tccctaactg gaaaggccca
     5761 gagaggattc agtaaaccaa tggaaaatcg aagccgggcg gcctatttgg tcggcccgcc
     5821 agccgtgtat ttgttgttcc ttttcatcct gccgttaggc attatggcaa tattcagctt
     5881 ccgggcaggg acgattggag cagccagcca gcaattcacc ctggataact atatcgattt
     5941 tatccagaat gccacctacc accggctact ctggcgctcg gccctgatcg cctttaccat
     6001 ctctgcggta tctgtcctat tcgcctaccc gctggcctat ttcctggtat tcattgccgg
     6061 cgagcggcgg atcaccttct taaccttgat catcgtgccc acctggacca gctacctgct
     6121 gcgcatcttc gcctggaaac tcatattggg ctctcaaggc ctgctcaata cgctgctttt
     6181 atggttgggt ttgatcgaac aggcttcgtc catcctgctt tacagccgca ccgcggtagt
     6241 gatcaccctg atttacgtct gggtgccctt cgttgccctg cctatcttcg ccgccctgga
     6301 gcgcattgac cgcaatttgc tggaggccgc cgccgacctg ggctgccgcc cttgggaggc
     6361 cttcctgcgc gtcaccctgc cactcagcat gccaggtgtg gtcgcaggct ttatgttcgc
     6421 cctcatccct accctgggag agttcgtcac accattgctg gtgggaggcg ggcaaggagc
     6481 gatgtacggc aacctgattc aagaccaatt cgtgcgcgct ttgaactggc caatgggatc
     6541 ggtgatgagc ctggccatgc tggtcatggt acttctgatc atctttgcct tcttgcgcgg
     6601 tggttcagcc gccaagctat tggatatata gatgaaacgc cagcattcat tctccctctt
     6661 cacgttgtat tactggctgg tgactgctgt cctctttctt ccgattattc tgctgatcat
     6721 attctcgttt aacaactcga tcaccctggt attcccgctg aaagggttta cactaaaatg
     6781 gtaccagcag ctaatacaaa actacgagct gatccgatct gtgcgcacca gccttctgtt
     6841 agggatcggc acctcactcg tagctaccgc actaggggct atgggggcta ttgccatggt
     6901 gcgcttcaac ttccccggca agagcttgtt catctcagtg gcagccatgc cgttag
//
LOCUS       DBNHOH010000937         5400 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1341818, whole genome shotgun sequence.
ACCESSION   DBNHOH010000937 DBNHOH010000000
VERSION     DBNHOH010000937.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5400)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1341818"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="AC16V7_16870"
     CDS             <1..472
                     /locus_tag="AC16V7_16870"
                     /inference="COORDINATES: protein motif:HMM:NF022059.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HIR6258849.1"
                     /translation="TLRQVIDEFGGGLLPGSQFNFALTGGAAGTIVPPSLLDVPIDYA
                     SAAMGVSLGAGAFLICDHTVSPIAFLRELLHFFTVESCGKCTPCRIGTQQSLLILNRM
                     TLGEGKTGDVQQLRQLADLMQQASFCGLGQSVAIPVKSALTHFAKQFQSSEQAQ"
     gene            492..3254
                     /gene="fdhF"
                     /locus_tag="AC16V7_16875"
     CDS             492..3254
                     /gene="fdhF"
                     /locus_tag="AC16V7_16875"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1578..1580,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HIR6258850.1"
                     /translation="MAINVTINEQTINAKEGQTILEAAQDHGITIPTLCYHKDLSPVG
                     SCRLCLVEVDKWRGQIAACTTQVAQDMIVRTETPALIQSRRLILEMLLQNYVDAGYAS
                     GDEKHDEFMAWVRHYQVALPEGSIPAQRYRVDSDPNPFLWVDLNKCILCTRCVRACAE
                     IQGRFVWGVGQRGYEAHIIAGANTDLLNARCESCGACVTYCPTGALDHKMSINLGHPD
                     KVVTTTCTYCGVGCQLDLNVKDNHVIRVTSNPNAPVNGNHLCVKGRYGYDFIHHPDRL
                     TKPRVRSYLLQDDSKPKGDLGEWVEVDWDTALEITARKLTHTRDTFGPDSIGVLTSAK
                     CLNEENYLMNKFTRQVIGTNNIDHCARLUHSSTVAGLATSYGSGAMSNSMDDIANHAQ
                     SMLVIGSNTTEQHPVFGSMIRHAILKRKAKLVVADPRKIDLTEYATLHLRQRPGTDVA
                     LINGIMYIILEQGWADQSFIEQRTEGFAEFQATVMQYPPQKVSQITRVPEEQLYQAAE
                     ILATNKPMAVMWAMGITQHIVGVRNVMDLANLQMLLGNIGVPGGGVNPLRGQNNVQGA
                     CDMGGLPNVYPGYQAVTLEAARQKFENAWGIPLSEKIGMTVTEMIPAAGDGSIRALYI
                     LGEDPVMSDPDSNHIRHCLESCDFIVLQEIFPSETAVFADVLLPGASFAEKTGTFTNT
                     ERRIQMVRSAIDPLGDARFDWQITSDLAKRILGSSSRKVGNGTHASWDFTDTSEIMDE
                     IAALTPSYAGISQARLERGERLQWPVKGPDHPGTPILHVGQFARGRGKFMPIDHVPPA
                     EMPDDDYPMLLSTGRVLYHWHGGEMTRRAEGLMEVYGQSLIEVNPEDATRLGLNGNQR
                     VRVTSRRGSIEAQAWVTERVPPGMVYANFHFPDASANELTIAALDPVAKIPEYKVCAV
                     KVESVA"
     gene            3270..3761
                     /locus_tag="AC16V7_16880"
     CDS             3270..3761
                     /locus_tag="AC16V7_16880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HIR6258851.1"
                     /translation="MDVRKIYEYALAREHEGKRFFETNAARMSHAAAVGAFKQLAAEE
                     QKHIDFITLQIAAFDRGLYSDMSVKGELEKGGLFSQRASLESIDQTISEAMVPDLPVL
                     RMAYLIERDFAEFYEMAAQKMEGEPKQVLSMLAQWERGHERYFKEWHDKAFEEYAKMP
                     WGG"
     gene            3859..5043
                     /locus_tag="AC16V7_16885"
     CDS             3859..5043
                     /locus_tag="AC16V7_16885"
                     /inference="COORDINATES: protein motif:HMM:NF027484.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HIR6258852.1"
                     /translation="MNPRIYRPIIALPLAFLLMIACNFFSPTAPPGEFDQGQLYTQAA
                     ETLSVQLTQVALFATATDLSKPTQTPTQIVLPSNTPLPTATNTPVPTATQFPTATRVP
                     PTSTPRPCLAARFVRDVTVPDGTKYAGGTSFTKTWELENVGSCTWTDDFELVFVKGDR
                     MSGDDNIPIDERVEPGETVRVSVDLLAPFTKGEYRGDWMLADDDGEEFGLGAGANKSF
                     WVSIRVTEVKEGVVLSFVDSYCSADWDSSAGDLPCPGKGTEPSGFVVRLEEPDQENRK
                     ENEPALWTNPEMEDDGWITGTYPAIKIKDGDRFIADIGCLDDSEDCDVIFRLNYKIGS
                     GPVKNLGEWHEVFDNLITRVDIDLSDLDGENVKFILTVTVNDDYDEDAAFWLQPRIQR
                     ED"
BASE COUNT         1398 a         1402 c         1361 g         1239 t
ORIGIN      
        1 gactttacgc caagtaattg acgaattcgg gggcggtctg cttccgggat cgcagttcaa
       61 tttcgccctt actggtggcg cggcaggcac tatcgtcccg ccctcactgc ttgatgtgcc
      121 catcgattat gcttcggcgg ccatgggtgt ctcgctaggt gctggagcgt tcctgatttg
      181 cgatcacacg gtatcgccta tcgcctttct aagggaatta ctccacttct tcacggtgga
      241 atcgtgcgga aaatgcactc cgtgccgtat agggacccag caatctctgc taatcttaaa
      301 tcgtatgacc cttggtgaag gcaaaaccgg ggatgttcag caacttagac aactagctga
      361 cttaatgcaa caagcttcat tctgcggtct aggacagtcg gttgcaattc cagtcaaatc
      421 agcgctaact cactttgcca aacagttcca gagctctgaa caggcacagt aatattttgg
      481 gagatgacaa tatggcgatt aacgtcacaa tcaatgagca aaccatcaac gccaaagaag
      541 gtcaaaccat tctggaggcc gctcaagatc acggaatcac tattccaacc ttgtgctatc
      601 acaaagatct ctctccagtg ggctcatgtc gcctatgcct agtagaagta gacaaatggc
      661 gcggccagat tgccgcttgc accacccagg tagctcaaga tatgattgtg cgtacagaaa
      721 cacctgcgct gatccaatcc cgacgcctaa ttctggaaat gcttttgcaa aattatgttg
      781 atgcgggcta tgcttcaggt gatgagaaac acgatgaatt tatggcttgg gtgcggcact
      841 accaggttgc gctacccgag gggagtattc ctgcccagcg ctaccgagta gacagcgatc
      901 ccaacccgtt cttatgggta gatctcaata aatgcatcct atgcacgcgc tgcgtgcgcg
      961 cctgcgccga gattcagggg cgttttgtct ggggggtggg tcagcgagga tatgaggctc
     1021 acatcattgc tggcgcaaat accgatctgc tcaacgcacg ctgcgagtct tgcggcgctt
     1081 gcgtaacgta ttgccctacc ggcgcgctcg atcataagat gtctatcaat ttaggtcatc
     1141 cagataaagt agtcaccacc acctgcacct actgcggggt aggctgccaa ctcgacctga
     1201 atgtcaagga taaccatgtc attcgggtga catctaaccc gaacgcaccg gtcaacggga
     1261 atcacttgtg tgtcaaaggg cgctatgggt acgattttat ccatcacccg gaccgtctta
     1321 ccaaaccaag ggtgcgtagc tatttactgc aggatgatag caaacctaaa ggagatttag
     1381 gggagtgggt cgaagtcgat tgggacacag ccctggaaat caccgcccgc aaattaaccc
     1441 atacccgaga tacatttggc ccggatagta tcggtgtcct gacctcggca aaatgcctga
     1501 atgaagaaaa ttatttgatg aataaattta cccggcaggt gatagggact aataatatag
     1561 atcactgcgc ccgtctctga cactcgagca ctgtggccgg tctggccacc tcatacggct
     1621 cgggcgccat gtccaactca atggatgata tcgccaatca tgcccaatcc atgctggtga
     1681 tcggctccaa cacgaccgag caacatccgg tgtttggcag tatgatccgc cacgcgattc
     1741 tcaagcgcaa ggccaaactg gtcgttgcgg accctcgcaa aatcgatctc acagagtacg
     1801 ccacgctcca cctgcgccaa cgtccaggta cagatgtagc cttaatcaat ggaataatgt
     1861 acatcatcct tgaacaaggc tgggcggacc aatcctttat tgagcaacgt actgaaggct
     1921 tcgcagagtt ccaagccacc gttatgcaat acccgccgca aaaggtatca cagattacac
     1981 gcgtgccaga agaacagctc taccaggcag ctgaaatact ggcaacaaat aaacccatgg
     2041 cggtgatgtg ggccatgggc atcacccagc atatcgtggg tgttcgaaac gtgatggacc
     2101 tggccaactt gcagatgctc ttgggcaaca tcggcgtccc cggtggcgga gtcaaccctc
     2161 tacgaggcca aaataacgta cagggtgcat gtgatatggg cggcctgccc aacgtttacc
     2221 ccggttacca ggctgtgaca ctggaagcag cgcgtcaaaa atttgaaaac gcctggggta
     2281 taccgctttc tgagaaaatc ggaatgacgg ttaccgagat gattccggct gccggcgacg
     2341 gcagcatcag agccctttac atactgggtg aagatccggt gatgagcgac ccagatagca
     2401 accatatccg ccattgtctc gaatcttgtg attttatcgt cctccaagaa atattcccat
     2461 ccgagaccgc ggtttttgcc gatgtgcttc tacccggcgc aagctttgcc gagaagaccg
     2521 gcacattcac caataccgag cgccgaatcc agatggttcg cagtgcgatt gacccattgg
     2581 gtgatgcgcg tttcgactgg caaatcacca gcgatttagc taagcgcatt ctgggaagta
     2641 gcagtcgcaa agtaggcaat gggacacacg catcttggga ctttacagat acatccgaga
     2701 ttatggatga aattgccgcc cttacgccaa gctacgcagg catcagccag gcgcgccttg
     2761 agcgtggtga acgactgcaa tggccggtta aaggacctga tcacccggga acgccgatcc
     2821 tacatgttgg tcagtttgcc cgaggtcgag ggaaattcat gcccatcgat cacgtacccc
     2881 cggctgagat gccagacgat gattatccga tgttactcag cactggccgc gtgctatatc
     2941 actggcatgg aggagagatg acccgccgcg ccgaggggct catggaagtc tatggacaat
     3001 ccttgatcga agtcaaccct gaagacgcaa cgcgccttgg actaaatggc aaccaacgtg
     3061 tgcgagtcac ttcccgcagg gggagcatcg aggctcaagc ttgggtgact gaacgtgtac
     3121 caccaggcat ggtttatgcg aattttcatt tccccgatgc atcagcaaac gaattaacta
     3181 tcgcagctct tgatcccgta gcaaaaattc cggaatataa agtctgtgca gtcaaagtag
     3241 aatccgttgc ataggagagg tatacagcca tggatgtccg aaaaatctac gaatacgccc
     3301 ttgctcgtga gcatgaagga aaacgcttct ttgagaccaa cgcggcccgt atgagccatg
     3361 cggcagcggt cggtgcgttc aagcaattag ctgctgagga acaaaaacat atcgacttca
     3421 tcactctaca gatagctgcc ttcgaccgag ggttgtattc tgatatgtcc gtgaagggtg
     3481 agctggagaa aggcggatta ttctcgcaac gcgcttccct tgaatcaatc gatcagacta
     3541 tttctgaggc aatggtcccc gacctgcccg tcctccgcat ggcatatctt atcgagcgcg
     3601 attttgccga attttacgag atggccgcac aaaaaatgga gggcgaaccg aagcaggtct
     3661 tgagcatgtt agcccagtgg gaacgcggtc acgagcggta cttcaaagaa tggcatgata
     3721 aagcctttga agagtatgct aaaatgccct ggggtggttg acgcggaaca aaaaacaatt
     3781 gtagatcgtc agtagcatag gccattagca gcacctaggc ctcatttatc ttgcgtgatt
     3841 tctgaggagc agatatttat gaacccgaga atttaccgcc caatcattgc cctaccactg
     3901 gcatttctac taatgatcgc ctgcaatttc ttttcgccga ccgcacctcc tggtgaattt
     3961 gatcaaggtc agttatacac ccaggcagct gaaacattaa gtgttcagct aacgcaagtc
     4021 gccttgttcg ctacggcaac tgatttatcc aagccgacac agacacctac ccaaatcgtg
     4081 ctgcccagta acacgccctt gcctaccgca acaaatacgc ctgtgcctac cgctacccaa
     4141 tttccgacag cgacacgtgt cccgcccacg tcgacccctc ggccttgtct ggctgctagg
     4201 tttgtcagag acgtcaccgt accagatggg acgaaatatg ccggaggtac gtccttcacc
     4261 aagacctggg agctcgagaa tgtcggatct tgcacatgga cggatgattt tgaactggtc
     4321 tttgtcaagg gggatcgtat gagcggtgat gataatatcc ctatcgatga aagggtcgaa
     4381 cccggcgaga cagtcagagt atcagttgat ctgttagccc ccttcacaaa aggtgaatat
     4441 cgtggtgatt ggatgctagc agatgatgat ggcgaagaat ttggcttagg tgccggagcc
     4501 aataaatctt tctgggtaag catccgtgtt acagaagtga aagaaggagt agtacttagc
     4561 tttgtagatt catactgctc tgcagattgg gacagcagtg ccggtgatct accttgccca
     4621 ggtaagggca ccgaaccatc tggctttgtc gttcgcttag aagaaccaga tcaagaaaat
     4681 cgaaaagaga atgagcccgc cctatggacc aatccagaga tggaagacga cggctggatc
     4741 actggcactt atccagccat aaaaattaag gacggagatc gttttattgc cgatatcggg
     4801 tgccttgacg acagtgaaga ttgcgatgtc atattcaggc tgaattacaa gatcggcagc
     4861 ggaccggtca aaaatttggg cgagtggcac gaagtatttg acaacttaat cacccgcgtc
     4921 gacattgacc taagcgatct ggatggagaa aatgttaaat tcattctcac ggtgaccgtg
     4981 aacgacgatt acgatgaaga tgccgcattt tggctacaac ctcgcattca gagagaagat
     5041 taatactaac ctaacgagaa aaacggcctc gcctctaatc agggtgaggc cgtttgttta
     5101 cctattagcc ccatttattt actgtttgac tttcccatgt taagatataa gcaatgaacc
     5161 cagatccttc ttcggcagca gtatacaatc cgttcctcca taatccacat cttgatgggt
     5221 ctacattctt ctgggaagga ggtcaagtgg gtgcactgct catccacgga ttgaccgcca
     5281 ccactgccga agtaagacca ttgggtcaga tattacacca acgccttggt tacacaatct
     5341 caggccccct gctacccggc cacaacacgc ggcccgatga cctcaatcgc ttccgctggc
//
LOCUS       DBNHOH010000938         1570 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_316561, whole genome shotgun sequence.
ACCESSION   DBNHOH010000938 DBNHOH010000000
VERSION     DBNHOH010000938.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_316561"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="AC16V7_16890"
     CDS             <1..740
                     /locus_tag="AC16V7_16890"
                     /inference="COORDINATES: protein motif:HMM:NF014003.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SAM hydrolase/SAM-dependent halogenase family
                     protein"
                     /protein_id="HIR6258853.1"
                     /translation="MKGVIWNISPDAQIADLSHTISPQNVLEASLILARSAPYFPAGS
                     IHLVVVDPGVGTNRRHIAARLGMQYFVGPDNGVISGLLEYAEAEDWTVEIVHTDKRQY
                     WLPKVSHVFHGRDVFAPIAAHLARGVPLYALGTPIHDPVRLSLPKPQTTPSGWQGEVI
                     HIDHFGNISTSIRVENLGYPDHVKVGLRDVIITGLVRTFGERPAGELVALYGSTGNLI
                     ISVVNGDAARRLNASVGDQVTVVLGEG"
     gene            743..>1570
                     /locus_tag="AC16V7_16895"
     CDS             743..>1570
                     /locus_tag="AC16V7_16895"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6258854.1"
                     /translation="MSEEYPLVVENLSFRYHSRPELAIQDVSFQLRRGELLLVAGASG
                     CGKTTLARCINGLIPRSYRGDRAGSVFIHGKEVSAMPLVEVAQVIGTLLQDPERQIVA
                     SNVWNEIAFGPENLGLSTQEIGERIDQVIARLNIEHLRGRDTFTLSGGEKQKVALAGL
                     LVMRPSILLLDEPLASLDPASAHEALAIFRSLADEGSTILLVEHRVEDALAARPDRLL
                     YMSAGRVRYLGTVDSLPPEIDHREVKLPAPWVVQRVHQTQPTQSALQPPPQAVAEGAP
                     "
BASE COUNT          305 a          439 c          464 g          362 t
ORIGIN      
        1 tgatgaaggg ggtcatctgg aatatttccc ccgatgctca gattgccgat ttgagtcata
       61 cgatcagccc gcagaatgta ttggaagcct ccctaattct agcgcgttct gccccttact
      121 ttcctgcggg cagtatccac ttggtggtgg tggatccggg tgtgggcacc aaccgccggc
      181 acattgccgc ccgcctgggc atgcaatact ttgtgggacc ggataatggg gtgatcagcg
      241 ggctgctcga gtatgcagag gccgaggatt ggacggttga gatcgtccac acggataagc
      301 gccagtattg gttgccgaaa gtcagccatg tctttcatgg cagggatgtt ttcgccccta
      361 tcgccgctca tttggcgaga ggcgtgccct tgtatgccct gggtacgccc atccatgatc
      421 cagtacggct gtctctcccc aagccacaaa ccaccccatc tggttggcag ggcgaggtca
      481 tccacatcga tcatttcggt aatatctcca ccagcatccg ggtagaaaac ctaggctatc
      541 ctgatcatgt gaaagttggc ctgcgcgatg tgataatcac gggtttggtg cgcactttcg
      601 gcgagcgccc tgccggtgag ctggtagcct tatatggcag cactggcaac ctgatcatct
      661 cggtggtcaa cggggatgct gcccggcgat taaacgcctc ggtaggagat caagtgactg
      721 ttgttcttgg tgaaggttga agttgagcga agaatacccg cttgttgttg aaaacctgtc
      781 tttccgctat cacagccggc ctgagcttgc catccaggac gtgtcattcc agctccggcg
      841 cggcgagctg ctcttggtgg caggcgccag tggttgcggc aagactaccc tggcgcgctg
      901 catcaacggg ctgatcccgc gcagttatag aggtgatcgc gctggatcgg tgttcatcca
      961 cggcaaggaa gtctctgcga tgccgctagt ggaggttgct caggttattg gaaccttgct
     1021 tcaagatcct gaacggcaga tcgttgcctc aaatgtttgg aatgagattg cttttggacc
     1081 agagaatctg ggactctcaa cccaagagat cggtgagcgt atcgaccagg tgatcgcccg
     1141 cttgaatatc gagcacttgc gcggccggga taccttcacg ttatctgggg gagaaaaaca
     1201 gaaagtcgct ctggctggcc tgttggtgat gcgcccctcg attctgcttc tggacgaacc
     1261 cctggcctct ctggatccgg cctcagccca tgaagccctg gcgattttcc gcagcctggc
     1321 agatgagggc tcgaccatct tgcttgtcga gcaccgcgtt gaagacgctc tggcagcgcg
     1381 ccccgatcga ttgctgtata tgtcagccgg acgggtgcgc tacttgggca ctgtggattc
     1441 gctcccccca gagatcgacc accgtgaggt caagctacct gctccatggg tggtgcaacg
     1501 agtgcatcaa acacaaccca cgcagtcggc gctgcagccc cctccccagg cagtggcgga
     1561 aggtgcgcct
//
LOCUS       DBNHOH010000939        10054 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1726497, whole genome shotgun sequence.
ACCESSION   DBNHOH010000939 DBNHOH010000000
VERSION     DBNHOH010000939.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10054)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10054)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10054
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1726497"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1930
                     /locus_tag="AC16V7_16900"
     CDS             <1..1930
                     /locus_tag="AC16V7_16900"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HIR6258855.1"
                     /translation="ITIQYWFFYYYNDWFNKHEGDWEMVQVMLDGQGEPEWVVYGQHH
                     GGTRRSWEQTNIEAGTHPVVFVASGSHANYFWGDEVYPTVQKIGNAKIEVVDHTGEVG
                     RVIPHVILIPETEDVQNDPLLWSGMTWLLFGGHWGETAEHKDFSGPLGPAYKGGQWEQ
                     PYAWGMAQPLDTDFWYTNRLRVEISGEDAKNSRVRLKTAKGEEITNAEMPGYFALSHT
                     NLNPLEEIIADIELPRNTPYNLDITWPDTVASRVNHFSFADLPLSLSGHISLTLTNDH
                     LPLLRVDGKTYELQPDENITEALSGYDPDWVWMAGSLSTNELVKGIGIGLLAAVLPAL
                     VYVSLLYWSDYYEKEPSRLLVSTFLWGALPALVLAVIMRIFYRLPPGLFTPNATRALW
                     VILLSPLTEEILKGVAVVYVAFRYRREFDNVLDGIIYGAMVGVGFAMTGNIISFIGSF
                     LTRGFAGLGSTIFIEGVLYSLDNALYSAIFGAGMGFYRLSQDRQRRYAVAIGAFALAV
                     VTHGLHNTLIQNAVDMSPMIVLLSWSGLSVLVIAMAWSLRRQRHCLELELIEELPGDM
                     YHTIVQPRLRLQTEWQILKSEGFAAWKLRRRLFQLCAEFAFKRMQARLFPDEAEIDAE
                     ANALRDEIRGILERYESQ"
     gene            complement(1942..3381)
                     /locus_tag="AC16V7_16905"
     CDS             complement(1942..3381)
                     /locus_tag="AC16V7_16905"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - obsolete cysteine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR6258856.1"
                     /translation="MTAPIIPTDHNYRDLFVGLDVNVPLLDGTTKRYINLDNAASTPS
                     FKFVQNLVDNFLSYYSSVHRGTGFNSQLSTHAYEEARHIVLRFLSADPATHICVFGKN
                     TTEAINKLARRFPFTPERDVVLISGMEHHSNDLPWRMRGAQNGQFDIKTIFIDLTPDG
                     QLDEVDFNNQLERYKDRLALVAVTGASNVTGYVNSIHRLAEKAHAAGALILVDCAQLT
                     PHRKVDMLSLDHSAHLDFVAISAHKMYAPFGTGALIGRRDIFEHGEPDMTGGGTVEIV
                     TKEDVVWAGPPDRDEAGSPNIVGAIALAAAIRQLEAVGMDAVGQHEADLTAYALEKLT
                     TVPGIAIFGDTRPAQTSTRLGVIPFELQGNSHFKAAAVLGYEFGIGVRSGCFCAHPYI
                     LHLLKLTPEETATARTEMLAGDKSNMPGLIRVSFGLYNTKDEVDSLVEALSSIQAGRY
                     HGNYTQNIATGEFTPEGWLPRFEEYFSFH"
     gene            3556..3993
                     /gene="mraZ"
                     /locus_tag="AC16V7_16910"
     CDS             3556..3993
                     /gene="mraZ"
                     /locus_tag="AC16V7_16910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIR6258857.1"
                     /translation="MFMGQYVHSLDVKGRLTIPSRYRALLEDGAYITQGFDHNLMVWT
                     KPAFEQISHRVSQGSITDPTSRLLRRFIYSSGDAMEMDRAGRILIPEFLRQVAQLDSE
                     VVVVGVGDYFEIWSPSLWAEQLASMQDAEANQQRFMAFDLSAD"
     gene            4020..4967
                     /gene="rsmH"
                     /locus_tag="AC16V7_16915"
     CDS             4020..4967
                     /gene="rsmH"
                     /locus_tag="AC16V7_16915"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR6258858.1"
                     /translation="MHRSVLYQEIIHELRPRQNGYYVDATVGAGGHAWGILQASSPSG
                     RLLGLDVDPQALELARHRLQEFGERAVLVRASYIALSEQLADLGWAEVDGILLDLGMS
                     SMQVDTAERGFSFLVEAPLDMRFDPDNPVRAADLVNELPESELADIIYHYGEERRARQ
                     VARAIVAARPIHTTVQLAQVVSRVVRAASRSKDRQQHGIHPATRTFQALRIAVNRELD
                     ALEAVLPQCIKILTTGGRLAVIAFHSLEDRIVKQFFRRESQDCICPPRQPVCTCGHKA
                     SLKEISRRPITPQSAEIEQNPRARSARLRVAEKLIGIEN"
     gene            5072..5548
                     /locus_tag="AC16V7_16920"
     CDS             5072..5548
                     /locus_tag="AC16V7_16920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258859.1"
                     /translation="MDRVQSLTQAYSQAPWRKQTMFIGLFLLVVIFAALVAGIYLDVT
                     ARTATIGREIQGMEEDIETLKLANADLESKLALLMSTEEMEKRARSMGFKSIEMDEAM
                     YVVVPGYISRRHVQLAPPPGPVKTVAASLPTAFTESLFAWFQRKVVPMRSQILEVR"
     gene            5548..7311
                     /locus_tag="AC16V7_16925"
     CDS             5548..7311
                     /locus_tag="AC16V7_16925"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR6258860.1"
                     /translation="MMSEHVWRYTLLGTIFSLLAFAIVGQMVRIQLNPEQVAYFMEVR
                     EANLGTYRTISPARGLIYDRDGHVMASNTKVYEVGVELAWVENPHTIALALNVVLGLD
                     YKDVLQAVSQDVSPSAVYVVLDKFVPQEKVDQLESYRAEIESNYTNRRDNDRPSLSGL
                     VYVPHLMRSYPEKDRASNILGFVTLEGEGNFGVEAKFNHLLAGDPKNVWIPRDPNLAE
                     EIPNIPDGASVILTIDRSIQRAVEDIVDDAVDDSGSDSGTIVVLAPQTGEILAMATTT
                     RLNLNEYWRYSEVFKGNTPFNRAVSEMYEPGSVFKILTMAAALDKGAVTPETEFLDTG
                     VFEIGGTYIYNWNRAAWGPQNMMGCLQHSLNVCLAWVSSELGAKDFYSYLRAFGIGHL
                     TGVDMAGEMPGRLKLPGDADWYDADLGTNAFGQGVAVTPLQLAVASAAIANDGKLMAP
                     HIVKAVVKDNQQFDTQPRLIGMPISSKTANTLTEMLASSLEKEASDALVEGYRVAGKT
                     GTAEIPTPYGYSSSATNASFVGWGPVDDPMFLVYIWLEKPTTSPWGSVVAAPVFQQVV
                     ERLVVLMEIPPDDVRRKIFSQ"
     gene            7313..8734
                     /locus_tag="AC16V7_16930"
     CDS             7313..8734
                     /locus_tag="AC16V7_16930"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIR6258861.1"
                     /translation="MLTLADVLEALTGMRPAGGQQIVTDASIDSRQSIPGSMFIALPG
                     ERVDGHEFVGAAFERGAHLALIERDLSPQFKVLDLRVKLSQPDLDKFDGPFCLLVSNS
                     LQALQQVARYWRRKLNLRVIGITGSVGKSTTKEVVASVLEQRYRTLKNPGNMNNEIGL
                     PLSILDLGPGHEVAVFEMGFYVPGEIALLCDLALPQVGVITNIGTVHAERAGTQEAIA
                     RGKAELVQSLPAAPEGVAILNYDDPWVREMAGQTSAQVLFYGLDPQADLWAEEVESMG
                     LDGIRFRLHFQEESIYLRVPLIGRHSVHTVLRAAAVGLVEGLTWQEIVSGLRAGHAQL
                     RLVAVRTERGALLLDDTYNASPESTLAALNLLEDLEGRRVAVLGDMLELGLYEAHGHE
                     IVGVRAAEVVDELVTVGERGRMIAMAARSTGFDRNKITELENSQEAIEYLRPKLDEKD
                     VVLVKGSHGMRMDRSVAALEYRE"
     gene            8731..9741
                     /gene="mraY"
                     /locus_tag="AC16V7_16935"
     CDS             8731..9741
                     /gene="mraY"
                     /locus_tag="AC16V7_16935"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR6258862.1"
                     /translation="MTQESAALALAVLSFALTVIWGTPLLRVLRYFKIGKSIRVEGPQ
                     RHFTKMGTPTMGGVLIVLPVLLITGLLNATTLLGLSVLGSSVLLPMAVMVSYALLGAL
                     DDWLGLRGRGEQGEGLRARTKFLIQLVLALAAALGLYYVLDVPNLYFPTVQGEIKLGF
                     WYVLIATFLIVSESNAVNLTDGLDGLAGLISATAFATFGAIALIQEQTFLARFCFTLV
                     GALFGFLWFNVHPAQLFMGDTGSLSLGATLAVVCLMTAQWPLLPIIAIIPFSNTLSVI
                     IQTMYFKFTRRRFGEGRRIFKMTPLHHHFELSGWSETQIVQRFWLISLLAGMIGVALA
                     VI"
     gene            9769..>10054
                     /gene="murD"
                     /locus_tag="AC16V7_16940"
     CDS             9769..>10054
                     /gene="murD"
                     /locus_tag="AC16V7_16940"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF045460.4"
                     /note="UDP-N-acetylmuramoylalanine--D-glutamate ligase;
                     involved in peptidoglycan biosynthesis; cytoplasmic;
                     catalyzes the addition of glutamate to the nucleotide
                     precursor UDP-N-acetylmuramoyl-L-alanine during cell wall
                     formation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR6258863.1"
                     /translation="MQNDFGGQRVLILGAARQGLALARYLVQQGARVTLNDRTPADKM
                     ANAMASLKDISESVRWVMGSHPVELLNDADLVCVSGGVPLANPIVVEAQAR"
BASE COUNT         2327 a         2303 c         2853 g         2571 t
ORIGIN      
        1 tatcacgatt caatactggt ttttttatta ttacaacgat tggttcaaca agcatgaagg
       61 ggattgggaa atggtgcagg tgatgcttga tgggcagggt gagccagagt gggtggttta
      121 tggccagcat cacggtggca cacgtcgatc ctgggaacag acaaatatcg aggctggcac
      181 gcatccagtg gttttcgttg cgtcgggttc gcatgccaat tacttttggg gggacgaagt
      241 ctatcctact gttcagaaaa ttggtaatgc aaagatagag gttgtggacc acactggtga
      301 ggttggacgg gttatccccc atgttattct gataccggaa acggaggatg tacagaatga
      361 tccgctctta tggtctggta tgacctggtt attatttggg ggacattggg gagaaacagc
      421 agaacataaa gacttttcag gccctttagg cccggcgtat aaagggggac agtgggaaca
      481 accctatgcg tggggcatgg cgcagccatt ggataccgat ttttggtata caaatcgtct
      541 aagggtggag atcagtggtg aagatgcaaa aaattcacgg gtgaggttga aaacagccaa
      601 gggggaggag atcaccaatg ctgaaatgcc agggtatttt gccctgtctc acaccaatct
      661 taatcctctc gaagagatca tcgctgacat tgaattgcct cgcaatacgc cctacaacct
      721 ggatattact tggcctgaca cagtggcttc tcgcgtcaat catttctctt ttgccgattt
      781 gccattgagc ctttccggcc atatttcact caccctaacc aatgatcatt tgcccctttt
      841 gcgcgtcgat ggcaagacct atgagttaca acctgacgag aatattaccg aggcactttc
      901 gggttatgat ccagattggg tctggatggc tggttcgcta tcaaccaatg agctggtcaa
      961 gggcatcggt attggactct tggcagctgt cttgcccgca ctggtctatg tgagcttgct
     1021 atattggtcc gactattacg agaaagagcc aagtcgtctt ctggtttcta cctttctatg
     1081 gggggcttta ccagctttgg tattggctgt cattatgcgg attttttacc gtcttccgcc
     1141 ggggttattt accccaaacg ctacaagagc gttatgggtt atcctgctct ctccattaac
     1201 tgaagaaatt ctaaagggag tagctgtagt ctatgttgcc tttcgctatc gccgagagtt
     1261 tgacaatgtg ttagatggca taatctatgg agccatggtg ggtgtcgggt ttgccatgac
     1321 tggaaatatc atcagtttca tcgggtcttt tttgacgcgc ggatttgctg gtttgggtag
     1381 cacgattttc atagaaggcg tgctttacag tttagacaat gcactctata gtgccatctt
     1441 tggtgcgggg atgggctttt atcgcctgtc tcaagaccgt caaaggcgtt acgctgtggc
     1501 tattggggct tttgctctgg cggttgtcac ccacggattg cacaacacat taattcaaaa
     1561 cgcggtagat atgagcccga tgattgtctt gctgtcctgg agcgggttat ccgttctcgt
     1621 gattgcgatg gcgtggtcat tgagacggca gcgccattgc ctggaattag agctgatcga
     1681 agaattgcca ggggacatgt atcatacgat cgtgcagccc aggctaagat tacagaccga
     1741 atggcaaatt ttgaaaagcg aaggatttgc tgcttggaag ctgaggcggc ggctcttcca
     1801 actctgtgca gaattcgctt tcaagcgtat gcaagcgcgc ttattcccgg atgaggcgga
     1861 gatcgacgcc gaagcaaatg cattgcggga cgaaataagg gggatattag aaaggtacga
     1921 gtctcaataa attgtattct attaatggaa agagaaatac tcttcaaacc ttgggagcca
     1981 cccctcaggc gtaaattcac ccgtggcaat attttgcgtg tagttgccat ggtagcgtcc
     2041 tgcctggatg ctgctcaatg cttcgactag cgaatccact tcgtcttttg tgttatacag
     2101 accaaaagaa acgcggatta agccaggcat gtttgactta tcccccgcca gcatttcagt
     2161 tcgtgcagta gctgtttctt caggtgttaa tttcaacaag tgaaggatgt aggggtgtgc
     2221 gcagaagcag ccgctgcgca cgccgatgcc gaattcatat ccgagaaccg cagcagcttt
     2281 aaagtgggaa ttgccttgca actcaaaggg gatcactccc aggcgagttg atgtttgggc
     2341 ggggcgagta tcaccaaata tggcaatgcc tggaacagtg gtcagcttct ccaaagcata
     2401 ggcagtcaga tcggcttcgt gttgtcctac ggcgtccata cccaccgctt cgagctggcg
     2461 aatggcagcc gccagggcga ttgcgccgac aatatttgga ctacctgctt cgtcgcggtc
     2521 aggaggacct gcccagacca catcttcctt ggtgacgatc tccaccgtac caccgccggt
     2581 catatccggc tcaccatgct cgaaaatatc ccgcctgcct atcagcgccc cagtgccaaa
     2641 gggtgcgtac attttgtggg ctgagattgc cacgaagtca aggtgggcgg agtgatcgag
     2701 ggacagcata tctacttttc gatgtggagt aagttgtgcg cagtccacaa ggatcagtgc
     2761 gcctgcggcg tgagcttttt ccgccaggcg gtgaatggaa ttgacgtagc cggtcacatt
     2821 gctggcacca gttacagcga ccaatgccag gcgatcttta tatcgctcta attggttatt
     2881 aaaatcgacc tcatccagtt gcccatctgg ggtgagatct atgaagatag tttttatgtc
     2941 gaactgcccg ttctgtgcgc cgcgcatgcg ccagggtaag tcgttagaat gatgttccat
     3001 tccgctgata agcaccacgt cccgttctgg ggtaaaaggg aaacgccgcg ctagtttgtt
     3061 gatggcttcg gttgtgttct tgccaaatac acaaatatgg gtcgctggat cggcgctcag
     3121 gaagcggagc acgatgtgac gggcttcctc gtaggcgtgc gtcgaaagct ggctgttgaa
     3181 accggtaccg cgatgcacgc tgctatagta gctcagaaag ttatccacaa gattctgcac
     3241 aaacttgaat gagggtgtag atgcggcgtt atccaggtta atgtaacgtt tcgttgtgcc
     3301 gtctaaaagc gggacattga catccaggcc gacaaataga tcgcggtaat tgtggtctgt
     3361 ggggatgata ggggcagtca tggtccacct ttctgacaaa tctgttggga aaatatattg
     3421 acctaaattg gtgggaaaaa gggcttgcaa ttcggacaat tttcgtatat actgtgtcta
     3481 tctgtggtag aaagtgggag aaagtggagc gccggtattt tccggcgttc aaccgttaac
     3541 gagaggttag aacatatgtt catgggtcag tatgtgcata gcttggatgt caaaggtcga
     3601 ttgacgatcc cttctcgcta tcgggctttg ctggaagatg gtgcatatat tacccaaggg
     3661 tttgatcaca atttaatggt atggaccaag cctgcttttg agcagatctc tcaccgtgtc
     3721 agccaaggca gcatcaccga cccaacatcc cgcctcctgc ggcgattcat ctactcaagt
     3781 ggggatgcaa tggaaatgga tcgggcaggg cgcatcctta tcccggagtt tctgcgtcag
     3841 gtagctcaac tggatagcga ggttgtggtt gtgggggtgg gggattattt tgaaatctgg
     3901 tctccttcgc tgtgggctga gcagcttgcc agcatgcaag atgctgaggc aaaccaacag
     3961 cgtttcatgg cgtttgattt atcagcggat tagcaccagg atatccgagt gagatatcca
     4021 tgcaccgttc agttctttac caagagatta tacacgagtt acgaccaagg cagaatgggt
     4081 actatgtaga tgccacggtg ggagctgggg gccacgcatg gggtattctg caagccagtt
     4141 cgcccagcgg acgattgctg ggcctggatg tggatcccca agcacttgag cttgcccgcc
     4201 atcgcttgca ggaattcggc gagcgagcgg tcctggttag ggcatcttac attgccctaa
     4261 gtgaacaact tgccgacctt ggctgggcgg aagtggatgg catcttatta gacctgggca
     4321 tgtcgtccat gcaggttgat actgccgagc gaggattttc ctttctggtg gaggctcccc
     4381 tagacatgcg tttcgacccc gataacccag tcagggcagc agacctggtc aatgaattac
     4441 ctgagtctga gctggcggat atcatttacc actatggcga ggaacggcgt gcacgccagg
     4501 tggcacgcgc catagtagca gcacggccga tccacaccac agtgcaactg gcacaggtgg
     4561 tctctagagt ggtaagggca gccagcagga gcaaggacag gcagcagcat ggcatccatc
     4621 cggcaacacg caccttccag gcactgcgca ttgctgtaaa ccgcgagttg gatgctctgg
     4681 aggctgtctt gccacagtgt ataaaaatct taacaacggg tggtcggcta gcggtaatcg
     4741 ccttccactc gctggaagat cgcatcgtga agcagttctt tcgccgcgaa agccaggatt
     4801 gtatctgtcc accaagacag ccggtgtgta catgcgggca taaggcttcg ctgaaggaga
     4861 tctcccgccg accgatcacg cctcagtcag cggaaattga acaaaacccg cgcgctcgca
     4921 gtgcccggct gcgcgtagca gaaaaactga ttggcattga gaattaattt agttgtaagg
     4981 taaatatctt gaacggcatt actttttggc atgacacttg caagcttgtt gggtaccgga
     5041 gaaatccagc caaatcctgc aggaggatgg aatggaccgc gttcaaagtt taacccaggc
     5101 atactcgcaa gccccctggc gtaagcagac catgtttatt ggcttatttt tactggtcgt
     5161 gatttttgct gcgctggttg ctggcattta cttagatgta accgcgcgta cagccaccat
     5221 tggtcgagag atacagggga tggaagaaga tatcgaaact ctgaaattgg caaatgccga
     5281 cctggagtcc aagcttgccc tgttgatgtc cacagaagaa atggaaaagc gcgcccgcag
     5341 tatgggcttc aagtccatcg agatggacga ggccatgtat gtggtagttc ctggctacat
     5401 ttcaaggcga cacgtccagc tggccccacc tccgggtccg gtgaaaacgg tggctgcaag
     5461 tctgccaact gccttcacag aatcgctgtt tgcatggttc caaagaaagg ttgtgccaat
     5521 gcgcagccag attttggagg tgcggtgatg atgagcgagc atgtctggcg gtataccttg
     5581 ctggggacga ttttctccct cttggcgttt gctattgtcg gccagatggt gcgtatccag
     5641 cttaacccag aacaggtggc atattttatg gaggtgcggg aagcaaacct aggcacctac
     5701 cgtacgatat ctcctgcacg cgggttgatc tacgatcgag acgggcatgt gatggcaagc
     5761 aataccaagg tctatgaagt cggggttgag ctggcctggg ttgagaaccc acacacgatt
     5821 gctctggcgc tcaatgtcgt tctcggattg gactataaag acgtcttaca ggctgtcagc
     5881 caggatgtct ccccaagcgc tgtctatgtt gtcctagaca aatttgtgcc ccaggagaag
     5941 gttgatcagc tagagagcta tcgcgctgag atcgaaagca attatacaaa tcgccgggat
     6001 aatgatcgcc cgagcttgtc tggattggtg tatgttccgc atttaatgcg cagctacccg
     6061 gagaaagatc gagcttctaa tatactgggc tttgtgacct tggaggggga gggaaatttt
     6121 ggcgtggagg ccaagttcaa ccatttgctg gcaggagatc cgaagaatgt ctggattccc
     6181 cgggatccaa acctagctga agagataccc aatatcccag atggggcgag cgtgattctg
     6241 accatcgatc gcagcattca aagagctgtg gaagatatcg ttgacgatgc cgtggatgat
     6301 agcggctccg attcgggcac cattgttgtg cttgccccac aaaccggcga gattctggct
     6361 atggcgacga ctacccggtt gaacctaaat gaatactggc gctattccga ggtgtttaaa
     6421 ggcaatacgc cgtttaatcg cgcagttagc gagatgtatg agccaggctc ggtatttaag
     6481 atcttgacga tggcggctgc tctggataaa ggggctgtga caccggagac agagtttttg
     6541 gatactggtg tctttgaaat tggcggcacg tatatctata actggaaccg ggctgcatgg
     6601 ggtccacaga atatgatggg ctgtttgcag cattcgttga atgtgtgcct agcctgggta
     6661 tcatccgaat tgggtgctaa ggatttctat tcttatctgc gggcttttgg aatcggtcac
     6721 ttaactgggg tagacatggc tggcgagatg cctggaaggt taaaattacc cggcgatgcc
     6781 gactggtacg atgccgacct gggcaccaat gcctttggcc aaggtgtggc agtaacaccg
     6841 ttacaacttg cggttgcatc ggcggcaatc gccaacgatg gcaaattaat ggcgccacat
     6901 atcgtaaaag ctgttgtaaa agataaccag caattcgata cccaaccgcg tctaatcggt
     6961 atgcccatct cgtccaagac ggcgaatacc cttacagaaa tgctggctag ttccttagaa
     7021 aaggaagcct ccgacgcctt ggtggaaggt tatcgcgtcg caggcaagac aggcactgca
     7081 gaaattccca caccctatgg ctattcctct agtgctacga atgcctcatt cgtgggttgg
     7141 ggtccggtgg atgacccgat gttcttggtg tacatctggt tagaaaagcc taccacttct
     7201 ccttggggat cggtcgttgc ggcgcctgtt tttcagcagg tagtagaacg tctggttgtg
     7261 ttgatggaga taccaccaga tgatgtgaga cggaagattt tttcgcaatg atatgctcac
     7321 tctggctgat gtccttgaag ccttgacagg catgcgacct gctggcggtc agcagattgt
     7381 tacggacgcc agcatcgact ctcgccagag cataccagga agtatgttta tagcattacc
     7441 tggagagcgt gttgatggac atgagtttgt cggtgcagca tttgaaaggg gtgcgcactt
     7501 agcgctaata gagcgagacc tctcgcctca gttcaaggtg ctcgacttgc gagttaagtt
     7561 gagccagcca gaccttgata agtttgatgg gccgttttgc ttgctcgtct caaatagctt
     7621 gcaggcatta caacaagtgg cacgctactg gcggcggaag ttgaacctgc gtgtgatcgg
     7681 gatcaccggc agcgtaggaa aatccaccac caaagaagtg gtggcgtccg tactggaaca
     7741 gcggtatcgc accttaaaaa atccaggaaa tatgaacaac gagatcggtc tgccgctctc
     7801 gattctagat ctgggaccag gacacgaagt ggcagttttt gagatgggtt tttatgtgcc
     7861 tggggagatt gctctcttat gtgacctggc gttgccacag gtaggcgtaa tcactaatat
     7921 aggcacggtt cacgccgagc gggctggcac gcaagaagca atcgcacgag gaaaagccga
     7981 gttggttcag tcgctgcctg cggcacctga gggtgtagcc atactgaatt atgatgaccc
     8041 ttgggtgcgc gagatggctg gacagactag tgcccaagtt ctcttctacg gcctggaccc
     8101 ccaagctgat ctgtgggctg aagaggtaga gagcatgggc ctggatggca tccgctttcg
     8161 gttacatttt caggaagagt cgatttacct gcgagtaccc ttgattggtc gccattcagt
     8221 gcatacggtt ttacgggctg cagcagtagg cctggttgaa ggattgacat ggcaagaaat
     8281 tgtgagtgga ttaagggcag ggcacgccca attacgcttg gtagctgtgc gcaccgaacg
     8341 tggggctctg cttctggatg acacctacaa cgcttcgccg gaatcgacac tggcggcgct
     8401 gaacttgctt gaagatcttg aaggtcgcag agtggcagtt ttgggcgaca tgctcgagtt
     8461 agggctttat gaggctcatg gccatgagat agttggtgtt cgtgctgcag aggtggtgga
     8521 tgagctggtt actgtgggag agcgtggtcg catgattgcg atggcagctc gctcgactgg
     8581 atttgatcgc aataagatca ccgagcttga aaattcccag gaagcgattg aatacttgcg
     8641 accgaaactc gatgagaagg atgtcgtatt agtgaaaggc tcacacggta tgcgcatgga
     8701 ccgcagtgtg gctgcactgg agtacagaga atgacccaag aaagtgctgc attagcgctg
     8761 gctgtgctca gttttgcgct aacagtgatt tggggtacac ccttattgcg ggtgttgcgt
     8821 tatttcaaga tcggtaaatc catccgcgtt gagggtccgc aacgtcactt taccaagatg
     8881 ggcaccccaa ccatgggtgg tgtgctaatt gtcctgccag tgctgctcat caccgggttg
     8941 ttgaatgcca ccacattact tggcttgtcc gttttgggta gttctgtgct attgccaatg
     9001 gccgttatgg taagttatgc cctgttaggc gctttggatg attggctagg tctgcgcggg
     9061 cgaggagaac agggagaagg tttgcgagcg cgaaccaaat tcttgatcca gcttgtctta
     9121 gccctggcag ctgcactcgg cttgtattat gttttggacg ttcccaactt gtattttcct
     9181 acagtacagg gagagatcaa gctgggattt tggtacgtgc ttatcgccac atttttgatt
     9241 gtgtccgagt caaacgcagt caacttgacc gatggcttgg acggactggc aggactgatc
     9301 tcagctaccg catttgccac tttcggggca atagcattga tccaggagca aacctttcta
     9361 gcccggtttt gttttactct agttggcgct ttgtttggct tcttgtggtt caatgtgcac
     9421 ccagcacagt tattcatggg cgatactggc tcactatcac tgggagctac actggcggtg
     9481 gtgtgtttga tgactgctca atggccactc ttgcccatca tcgcaatcat accgttcagc
     9541 aatacgctca gtgtgattat tcagaccatg tattttaagt ttacccggcg tagatttggc
     9601 gaaggaaggc gtatcttcaa gatgacacct ttacaccacc attttgagct ctctggatgg
     9661 agcgaaaccc agatcgtaca gcgtttctgg ttgatcagcc tgctggccgg aatgatcggt
     9721 gtggcattgg ctgttattta aatatgtttt ttcggaacgt tagacaggat gcaaaacgat
     9781 tttggcggtc aaagagtgct gatattggga gcggcccggc aagggcttgc tttggcgcgt
     9841 taccttgttc agcaaggagc gcgcgtgaca cttaatgacc gcacaccggc tgacaaaatg
     9901 gccaatgcga tggcttcact taaggatatc agcgagtctg tacgctgggt tatgggaagc
     9961 cacccggtag agttacttaa tgatgccgat ctggtttgcg tttcaggagg cgtacccctg
    10021 gcgaacccga ttgttgttga agcacaggcg agag
//
LOCUS       DBNHOH010000940         2176 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1021988, whole genome shotgun sequence.
ACCESSION   DBNHOH010000940 DBNHOH010000000
VERSION     DBNHOH010000940.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1021988"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1286
                     /gene="gcvPA"
                     /locus_tag="AC16V7_16945"
     CDS             <1..1286
                     /gene="gcvPA"
                     /locus_tag="AC16V7_16945"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HIR6258864.1"
                     /translation="RIEDLFNAVPGAHRFPDLKLPPALSEMEAMAGMQDLAWSNENAR
                     DLVCFLGAGAYNHYIPAAVDMLLRRGEFYTAYTPYQAEISQGTLQAIFDYQSLIAALT
                     GMEVSNASHYDGATATAEAITMAQANFRGKRTKFVLSPALHPHYRQTVHTYNHGSELI
                     FVGEDVDVTASPESLMPLIDENTALVMVQYPDFFGRIYDYTALAEATHNAGALFAVAV
                     NPLSLGLLKPPGEFGADIVVGEGQPLGIPLSYGGPYLGIFATRKEYVRKMAGRLVGET
                     VDNRGQRAYVLTLTAREQHIRREKATSNICTNQGLIALASAIYMALLGKHGLRQVAEL
                     CYHKAHYAARKLGAIRGYQLWNQTPFFHEFVLRCPSSTEEINAHLLDHGILGGYDLGQ
                     AYPKLKNHLLIAVTEMNSKEDIDLLAEVLSEVSHG"
     gene            1279..>2176
                     /gene="gcvPB"
                     /locus_tag="AC16V7_16950"
     CDS             1279..>2176
                     /gene="gcvPB"
                     /locus_tag="AC16V7_16950"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HIR6258865.1"
                     /translation="MGEPLLCEMSVPGRVGFRYPAPDVPMEPLPEALVRSNLPLPELY
                     EVDVVRHFTRISQMNHSIDTGFYPLGSCTMKYNPKVNEETARLPGFAHVHPLQPVETV
                     QGSLALMYDLQNWLKEIGGFAGVTLQPAAGAQGELTGVLILRAYHEKRGQTQRNKILI
                     PDSAHGTNPATSAMSGMRVVEIKSDARGNVDLEALRLECDDTLAGLMLTNPNTLGLFD
                     ENVEEVIRMVHEAGGLVYGDGANMNALLGIVRPGDLGFDVLHYNLHKTFSTPHGGGGP
                     GSGPVGVAAHLVDFLPGPIVGII"
BASE COUNT          486 a          615 c          585 g          490 t
ORIGIN      
        1 agcgcattga ggatcttttc aatgcagttc ccggtgctca tcgcttcccg gatttgaaac
       61 tacctcctgc cctatcggag atggaggcta tggccgggat gcaagacctg gcgtggtcga
      121 atgagaatgc ccgtgacctg gtgtgttttc ttggggctgg ggcgtataat cactacatcc
      181 ctgccgccgt ggatatgctc ctgcgacgcg gggagttcta tacagcctac actccctacc
      241 aggcagaaat ctcgcagggc actcttcagg ccatatttga ctatcaaagc ttaatcgctg
      301 cactgaccgg catggaagta tccaacgcct cccattatga cggcgctact gcgaccgccg
      361 aggcaatcac catggcacaa gctaactttc gcggcaaacg gaccaagttt gtcctctcac
      421 cagccttgca tccgcactac cgccagacgg tgcacaccta caatcatgga tctgaattga
      481 tttttgtggg ggaggatgtt gatgtcacag ctagtccgga gagcctgatg cccctgattg
      541 atgaaaatac agccctggtg atggtacagt atccagattt cttcgggcgc atctacgact
      601 atacagccct ggcagaggca acccacaacg ccggagcctt gttcgcagtg gcggtaaacc
      661 cactttcttt ggggttgctg aaaccccctg gcgagtttgg tgctgacatc gttgtgggtg
      721 aaggtcaacc attgggcatc ccgctttctt atggtggacc gtatctaggg atattcgcaa
      781 ctcgcaagga atatgtgcga aaaatggcgg ggcgcctggt aggagagacg gtagataatc
      841 gcggacagcg cgcctatgta ctcactttga ccgctcgaga gcaacacatc cgccgcgaga
      901 aagccacatc caatatctgc accaaccagg ggctgatcgc cctcgcctca gccatctaca
      961 tggcgctgct gggcaagcat ggtttgcgcc aggtagctga gctgtgctac cacaaagccc
     1021 actacgctgc ccgcaagctg ggtgcgatac gcggctatca gctttggaat caaacgccct
     1081 tcttccacga gtttgtactc cgctgcccat catccaccga ggagatcaac gctcacctcc
     1141 tggatcacgg aattctaggc ggctatgatc ttgggcaagc ttatcctaaa ttgaaaaacc
     1201 acttattgat tgcggttacc gagatgaaca gcaaagaaga tattgatctg cttgcagaag
     1261 tcctgtcgga ggtgtcccat gggtgagcca ttactttgtg agatgtctgt acctgggcga
     1321 gtcggttttc gctacccagc acctgacgtt cccatggagc ccttgccaga agcactggtg
     1381 cgtagcaacc tgcctctacc agagctatac gaagtcgatg ttgttcgcca cttcacgcgt
     1441 atctctcaaa tgaatcacag tatcgatact ggcttctacc cccttggctc ctgcacgatg
     1501 aaatacaatc ccaaggtaaa cgaagaaaca gcccggctac caggttttgc ccacgtgcat
     1561 ccattgcaac cggttgagac ggttcaagga agcctggctt tgatgtatga cttgcagaat
     1621 tggctcaagg aaatcggggg ctttgcaggt gtgaccctgc aacctgccgc tggcgcccag
     1681 ggtgagttga ccggagtgct aatacttcgc gcctaccacg aaaagcgcgg acagacacag
     1741 cgcaacaaaa tactgatccc cgactcggcc cacggcacca acccggccac ctcagccatg
     1801 agtggtatgc gcgtggtcga gattaagtcc gatgcgcgcg gcaacgtcga cctggaggct
     1861 ctgcgtcttg agtgcgatga cacgctggct ggattaatgc tgaccaaccc caataccctg
     1921 gggctcttcg acgagaacgt agaagaagtc atccgcatgg ttcacgaagc tggagggctt
     1981 gtttacggcg atggcgccaa catgaatgcg ctgttgggta tcgtacgccc cggagacctg
     2041 ggcttcgatg tgctgcacta taatctgcac aagaccttct ccacaccaca cggcggcggt
     2101 ggtcccggct ccggtccggt aggtgtggct gcccacctgg tagattttct gcctggccct
     2161 attgtgggca ttatcg
//
LOCUS       DBNHOH010000941         1956 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_444027, whole genome shotgun sequence.
ACCESSION   DBNHOH010000941 DBNHOH010000000
VERSION     DBNHOH010000941.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_444027"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="AC16V7_16955"
     CDS             complement(<1..832)
                     /locus_tag="AC16V7_16955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6258866.1"
                     /translation="MKTIPLWTDEFPKPDDIPVSRLVEKADVAVIGSGYTGLNAALVL
                     ANSGGKVAVLEQESIGWGASSRNGSMLTTGLKSPITQIIRRYGIDVARHLWKWSLDAI
                     DHVEKIIIRENIACDFSRPGHAYLAYKPHHYDKIKAYGELLARMFGYEGFEYIDPKDM
                     HQEIGSDAFYGAMTEAYSGLLQPAKYVYGLARAASRAGVLLVDKCRVTGISRRGNGFA
                     LQTSLGEMEAKEVLVATGGYTTNLVPSIRQGIFPVGSYIIVTEPLSADLQAELSPKRR
                     A"
     gene            complement(880..1890)
                     /locus_tag="AC16V7_16960"
     CDS             complement(880..1890)
                     /locus_tag="AC16V7_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6258867.1"
                     /translation="MGRTVNLTTNNVEPGETLVEVQNLVKFFPIRKGVLQRVVDWVKA
                     VDDVSFGIRKGETLGLVGESGCGKTTLGITMLRLLQPTSGRVEIEGVNIYDLSTEQLK
                     AIRRKMQIIFQDPYASLNPRKPIGESIMAGLNIHGIGTRQERVDIMLDLLRKVGLEEY
                     YARRFPHEFSGGQRQRIGIARALALQPEFVVCDEPISALDVSIQSQVLNLLKDLQDEF
                     HLTYLFIAHNLSVVEFISDRVAVMYLGKIVELAPREALFSNPLHFYTQAIMSAIPIAD
                     PKAQSQRIILEGEVPTPINPPPGCHFHPRCPWAMEECSVEEPPFIEISPEHWVACWHV
                     GP"
BASE COUNT          489 a          550 c          436 g          481 t
ORIGIN      
        1 acgcgcgtct cttcggactg agctccgctt gcagatcagc actcaaaggc tcagtgacga
       61 tgatataact gcccaccggg aaaatccctt gcctgatgct tgggaccagg ttggtcgtat
      121 acccgccagt cgccaccagc acttctttag cctccatttc acccagggat gtctgcaagg
      181 caaacccatt tcctcggcgg gaaatacctg tgacccgaca cttatctacc aacaataccc
      241 ctgcccggga tgcagctctc gctaatccat acacatactt ggctggctgc aacaagccag
      301 aataagcctc cgtcatggca ccgtagaagg catcgctgcc aatctcctgg tgcatatctt
      361 ttggatcgat atattcaaag ccctcatagc caaacatccg ggctagcagc tcgccatagg
      421 ctttgatctt gtcgtagtga tgaggtttat aggctagata ggcatgaccc gggcgcgaga
      481 aatcacacgc aatgttttct cggatgatga tcttttcaac atggtcaatc gcatccaggc
      541 tccacttcca gagatgacga gcaacatcga tgccatagcg tcgaatgatc tgcgtaatcg
      601 gagatttcag gcctgttgtg agcatactcc cgtttcggct gctcgctccc cagccaatac
      661 tttcttgctc cagcacggcc accttccccc cagagtttgc caagaccagg gctgcattca
      721 gtccagtata tccagacccg atgaccgcca catcagcctt ctccactaag cggcttactg
      781 gaatatcatc cggttttggg aactcatctg tccaaagtgg tatggttttc atcactcagc
      841 ctataatggc caataattta tgtgtatatc ccaagaatgt cagggtccca catgccaaca
      901 cgccacccag tgctccggtg atatctcaat aaaaggaggc tcctctaccg aacattcctc
      961 catagcccat gggcagcggg ggtgaaaatg gcaccctggc ggagggttaa tgggggtcgg
     1021 cacctcgcct tctagtatga tccgctggct ctgcgctttt ggatcagcaa tgggtattgc
     1081 cgacataatc gcctgagtgt agaaatgcaa gggatttgag aaaagcgcct ctcgaggcgc
     1141 taactcaaca atcttgccaa gatacatcac agcgacccta tccgatataa attcaactac
     1201 agacaggttg tgagcgatga ataggtaggt caagtgaaat tcgtcttgta agtccttcaa
     1261 caggtttaaa acctgcgatt ggattgagac atccaatgcg gaaatcggct catcacatac
     1321 aacaaactcg ggttgtaaag ccagcgcgcg agcgattccg atcctctgcc tttggccacc
     1381 agagaattca tggggaaagc gacgagcata atactcctcc aatcccactt ttcgaagaag
     1441 gtcgagcata atgtccacgc gctcttgccg agtgcctata ccgtgaatgt taaggccagc
     1501 cataatcgac tcgcctatcg gtttccgagg gttgagagag gcgtatgggt cctggaatat
     1561 gatttgcatt ttccggcgga ttgctttcaa ttgttcggtg ctcaaatcat aaatattcac
     1621 gccttcaatc tcaacccgac cgctggtcgg ctgcaacaaa cgcagcatgg ttatgccgag
     1681 tgtggtcttg ccacatccag actcgcctac cagacccagc gtctcaccct tgcggattcc
     1741 gaagctaaca tcatcaacag ccttcaccca atcaacaact ctctgaagta ccccctttcg
     1801 aatcgggaaa aacttgacca aattctgcac ctcaacaagg gtttctccag gttcaacgtt
     1861 atttgtggtg aggttcacgg ttcgtcccat tttgtctccc aaacaataag atcagcaaca
     1921 acacttttca ttcgaatccg tttttttctg ataccc
//
LOCUS       DBNHOH010000942         3540 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_59210, whole genome shotgun sequence.
ACCESSION   DBNHOH010000942 DBNHOH010000000
VERSION     DBNHOH010000942.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_59210"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /locus_tag="AC16V7_16965"
     CDS             complement(<1..1247)
                     /locus_tag="AC16V7_16965"
                     /inference="COORDINATES: protein motif:HMM:NF012364.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HIR6258868.1"
                     /translation="MEFSGWLLDVYADPHDGVALWLLDERDGSRRRLRQALPVAFHAA
                     GTAARLRQLWRYLQSEGLNGRVPVTLSRSERRELFSDQPVSVLTAQVSQPAAQPALFR
                     KVSQAFPDLTYYDADLSPALHHAARYGAFPLARCRVDADEQGQVQSLEVLNTPWDLDP
                     CAPPLRVLSLEPDVDPFHAAPTHILARAQRACYRLALPGPHAQGKAGIRPLLVNLRAI
                     FMRHDPDLLLTTWGDTWLLPYLLELSEHSGLSLPLNRDPAFGPLYRPERTYFAYGQVI
                     YRGQQVHLFGRWHVDGHNAVLYHDYGMEGIFELARVTSLPVQAVARLSPGSGISAMQM
                     LTALREGVLVPWHKQQAERPKTARELLRSDQGGLVYQPVIGLHKDVAEIDFVSMYPSI
                     MARFNISPETVKKEPHLSVLPDSG"
     gene            complement(1254..1577)
                     /locus_tag="AC16V7_16970"
     CDS             complement(1254..1577)
                     /locus_tag="AC16V7_16970"
                     /inference="COORDINATES: protein motif:HMM:NF053320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF8156 family protein"
                     /protein_id="HIR6258869.1"
                     /translation="MGRTLPSITQVFLQEQESLSRFRRALRRSDQLALDDLFAAARQH
                     LAAAAYAGHALPFEVFLLAMLLEEHKMVLRLRSALEEMQAPGYTPASPPEQIGPQAEL
                     FKLKP"
     gene            1652..2242
                     /locus_tag="AC16V7_16975"
     CDS             1652..2242
                     /locus_tag="AC16V7_16975"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR6258870.1"
                     /translation="MNQRIDGLTLVSLALILFMGFNVLRDRASTAHASSQLATGSQTA
                     PSLPASTAQPTEAEMLEISAPYEDYILTQGPHGTSYGHKAIDLAAGKGEAVLSPINGI
                     VTERYVDEYDNPTLVIENDIYRVTLLHGKYTVSVGDRLQIGQPVGKESNLGYTADIQG
                     RPCNGRKKCGYHTHLNIYDKRLQANVNPLDLIGATP"
     gene            complement(2243..2647)
                     /locus_tag="AC16V7_16980"
     CDS             complement(2243..2647)
                     /locus_tag="AC16V7_16980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258871.1"
                     /translation="MMFDDFEKYFKELSPDAFKRRIPIILYRKGGDRTDWTTPGGQVF
                     APVSTIVMIGCVEWTGASATSGDVVQAFPVHYYGEPLIFIQPRVTTPSGAFLMGYALS
                     AGDAVEIYWKSDVAVTMARFAWIAFGGQLVSP"
     gene            complement(2652..3311)
                     /locus_tag="AC16V7_16985"
     CDS             complement(2652..3311)
                     /locus_tag="AC16V7_16985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258872.1"
                     /translation="MTTLATFRSRIRAMLIDASGTIYADATVDEALRQALAEYNNALP
                     LERETVLILPGDGREIALNGIADLRAVTDVYWPFDSLDEPWPPNQVKGFYVFWDDTRP
                     VLYLNQIDGDGPQQDDELRLWYATNHTIDGLDSATETTIPKEHTSLLVAGAAGHAALM
                     RTVDLTEVSSADQYMTGLLGVWAQRVLKEFRAALEAIKRWKARAGAPYTQGWKLDKWD
                     D"
     gene            complement(3337..>3540)
                     /locus_tag="AC16V7_16990"
     CDS             complement(3337..>3540)
                     /locus_tag="AC16V7_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258873.1"
                     /translation="DQVKVYVDGAQSGSTQTGLNTWTGNLALAILGASSSAPAFPWNG
                     WLAHALLLDRAASAMEVAKCATV"
BASE COUNT          780 a         1052 c         1052 g          656 t
ORIGIN      
        1 ccactgtcgg gaaggacaga caggtgaggt tctttcttca ctgtctcggg cgaaatattg
       61 aagcgcgcca taatgctggg gtacatagag acgaagtcga tctccgccac gtctttgtgc
      121 aggccgatca ccggctggta caccaggcct ccctggtcag agcgcaacag ctcccgggcg
      181 gtcttagggc gctcagcctg ttgcttatgc cagggtacca gcacgccctc ccgcaaggct
      241 gtgagcatct gcatcgcgga gatgccgctg cccggcgaga gacgcgccac cgcctgcact
      301 ggcaggctgg tcacgcgcgc cagctcaaag atgccctcca tgccgtaatc gtggtagagc
      361 acagcgttgt gcccatccac gtgccaacgg ccaaacaggt gcacttgctg gccacggtag
      421 atcacctgcc cataggcgaa gtaagtgcgc tccgggcggt agagtggtcc aaaggcaggg
      481 tcgcggttga gcggcaggga caggccgctg tgctctgaga gctccagcag gtaaggcagc
      541 aaccaggtat cgccccaggt ggtcagcagc aagtcggggt cgtgccgcat gaagatggca
      601 cgcaggttca ccagcaatgg gcggatgcct gccttgccct gtgcatgcgg tccggggagc
      661 gccaggcggt agcaggctcg ttgcgcgcgc gccaggatgt gggttggggc agcatggaag
      721 gggtctacat ccggctccag ggaaaggacg cgcagaggag gtgcgcaggg atctaaatcc
      781 cagggtgtat tcaggacctc cagggattgc acctgcccct gttcgtcagc atccacacgg
      841 cagcgcgcca gggggaaagc accgtagcga gccgcgtggt gcaaagccgg ggaaaggtca
      901 gcatcataat aggtcaggtc gggaaaagcc tgagaaacct tgcggaacaa agctggctgg
      961 gcagccggct ggctcacctg cgcagtgagc acagacacag gctggtcgga gaacagctcg
     1021 cggcgctcgc tacgggaaag ggttacaggc accctgccat tcagcccttc tgattgcagg
     1081 tagcgccata attggcgcag gcgcgctgct gtacccgcgg cgtggaaagc caccggcagc
     1141 gcttgccgca ggcggcggcg agagccatcg cgctcgtcca gtagccagag cgcaacgcca
     1201 tcatgtggat cggcgtatac gtccagcagc cagccagaga attccattga cgcctatggc
     1261 tttagcttga atagctcagc ctgtgggcct atctgctctg gagggctagc cggggtgtag
     1321 cctggcgctt gcatctcctc gagggcagag cgcaatctta gcaccatctt atgctcctct
     1381 aatagcatgg ccaacaagaa gacctcgaaa ggcagggcat gacccgcata cgccgcagcc
     1441 gccaggtgct ggcgtgcggc ggcgaacagg tcgtccagcg ccagttggtc gctgcgccgc
     1501 agggcgcgcc ggaaacgaga caaagactct tgctcttgca ggaaaacttg tgtaattgat
     1561 ggaagtgtac gtcccatagc atacctcctg attcatggta taattagaac aaatattcta
     1621 ttttgtggag atgtttactc gcaccatagc tatgaaccag cgtattgatg gtctcaccct
     1681 tgtatccctg gcactgatcc tgttcatggg cttcaacgtc ctgcgagatc gagccagcac
     1741 ggcgcacgcc tcctcccaac ttgcaacagg cagccagacc gcaccgtcct tgccggcttc
     1801 caccgcccag cccaccgagg cagagatgct ggagatttcc gcaccctacg aggactacat
     1861 cctcacacag gggccgcacg gaacctccta tggccataag gcgatcgacc tggctgctgg
     1921 caagggagag gcggtgcttt cgcccatcaa cggaatcgtc actgagcgct acgttgatga
     1981 gtacgacaac ccgacacttg tgatcgagaa tgacatctac cgtgtcactc tgctgcatgg
     2041 caaatacacc gtctcggtag gcgatcgatt gcagatcggc cagccggtgg gcaaggagag
     2101 caatctgggc tacaccgccg atatacaagg cagaccctgt aatggacgca agaagtgtgg
     2161 ttaccatacg catctgaaca tctacgataa gcgcctgcag gccaatgtca acccactgga
     2221 cctgatcggc gcaaccccat gatcacggcg aaactagctg tccgccaaaa gcgatccagg
     2281 caaagcgggc cattgtaaca gccacatcgg atttccaata aatctccact gcatcacccg
     2341 ccgaaagggc gtaccccatc agaaaagcgc cggatggcgt ggtcacccgc ggctggatga
     2401 agatcaatgg ctcgccataa taatggactg ggaatgcctg gacaacatct ccactcgttg
     2461 cagaagcgcc cgtccattca acacaaccga tcatgacgat cgtcgaaacc ggggcaaaca
     2521 cctggccgcc cggcgttgtc caatctgtgc gatctcctcc cttacgatag agaataatcg
     2581 ggatacgccg tttgaaggca tctggcgaaa gctctttgaa gtacttttcg aagtcgtcaa
     2641 acatcatctg atcaatcgtc ccatttatcg agcttccaac cctgggtata tggggcaccg
     2701 gcgcgtgcct tccacctctt gattgcctca agagcagcgc ggaactcctt gagcaccctc
     2761 tgcgcccata cgcccagcaa gccggtcata tactgatccg cggaagagac ctccgtaaga
     2821 tcaaccgtgc gcatcagcgc cgcgtggcct gcagcgcccg ccaccagcag cgaggtgtgc
     2881 tccttgggta ttgtcgtctc ggtggcggaa tccaggccgt cgatggtatg gtttgtggcg
     2941 taccacaagc gcagctcatc gtcttgctga ggcccgtcgc cgtcgatctg gttgagatag
     3001 agcaccggtc gggtatcatc ccaaaagaca tagaaaccct tcacctggtt aggtggccag
     3061 ggctcgtcta agctatcgaa cggccagtac acatcggtaa cggcgcgcaa atctgcgatc
     3121 ccattcaaag cgatctcgcg cccgtcgccc ggcaagatca aaacggtctc cctctcgagt
     3181 ggcagggcat tgttgtactc cgccagggcc tggcgcaagg cttcatcaac cgtggcatcg
     3241 gcgtagatgg tgccagaagc gtcgatcaac atagcgcgga tgcgagagcg aaaggtggca
     3301 agtgtggtca taggttaatc ccacaggcag atagcctcat acggtggcgc acttggcgac
     3361 ctccatcgcg ctggcggcgc ggtcgagcag caaggcatgg gcaagccagc cgttccacgg
     3421 aaaggcgggg gcggaagagc tggccccgag gatggcaagc gcgaggttgc ctgtccatgt
     3481 atttagcccc gtttgcgttg aaccagactg agcgccgtcg acatagactt tcacctgatc
//
LOCUS       DBNHOH010000943         1578 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_123814, whole genome shotgun sequence.
ACCESSION   DBNHOH010000943 DBNHOH010000000
VERSION     DBNHOH010000943.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_123814"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="AC16V7_16995"
     CDS             complement(<1..315)
                     /locus_tag="AC16V7_16995"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HIR6258874.1"
                     /translation="MVKRLIVVAGNIGVGKTSLTERIGARLGWKTAFESVSDNPYLPD
                     FYADMRTWSFHLQIFFLGHRAKQYLELASMPESAILDRSIYEDAYIFARALHHLDNLN
                     ERD"
     gene            complement(569..757)
                     /locus_tag="AC16V7_17000"
     CDS             complement(569..757)
                     /locus_tag="AC16V7_17000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258875.1"
                     /translation="MSYVSTQNRGYVFAAFLGAAFGGVFVMLATKAIPKMMSQMMAGM
                     MQNMMSQMSAEGCNPAEM"
     gene            complement(845..>1578)
                     /locus_tag="AC16V7_17005"
     CDS             complement(845..>1578)
                     /locus_tag="AC16V7_17005"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015000.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIR6258876.1"
                     /translation="AYLRSEPITPTQYIAHRGGPIHAPENTLSAFRTAIQQGVDWLEF
                     DVQMTKDDELVVIHDESVDRTTNGMGEVRNLTLDQIRALDAGSGEQVPTFEDVVRLAK
                     MNGVKILPETKSAHLYPGIETKLMQELEELDYVDQTVVQSFEPASLDTLHRLNPDLNF
                     CALYGLWQFDISNPPADSQFVCPMAEMVLIFPSMIQQAHREGRQVFVWFGVMEHPLMF
                     RAMRFFGADGLMSDDPLLLVQSVAP"
BASE COUNT          425 a          404 c          327 g          422 t
ORIGIN      
        1 atcacgttca ttaagattat ccaagtggtg tagcgcccgg gcaaatatat aagcatcctc
       61 gtaaatacta cgatccagga ttgccgactc tggcatgctt gccaactcca agtattgttt
      121 tgcacggtgc ccgaggaaaa atatctgtag atggaacgac caggtgcgca tgtcagcata
      181 gaaatcaggc agataaggat tatctgacac cgattcgaaa gcggttttcc agcccagacg
      241 ggcaccaatg cgctccgtaa gggatgtctt accgacccca atattgccag caacaacgat
      301 gagacgtttt accatttaaa tctccttaag ttttttccca gcctacgact tgcatagcac
      361 aatccatgtt gtctctatta ttagtttgat catcaattgt gctataaccc aatgaccatt
      421 gacgctcgat aaacacaccc accgaggtag ccagttgatg attatagcat cacccaactg
      481 tatccagatt gttattccag ctgatcgatg actacccccc cggtacagac acttctggga
      541 cttcgacgaa gcccgccatc atacgttgtc acatctcggc tggattacaa ccttctgcac
      601 tcatttgaga catcatattt tgcatcatgc ctgccatcat ttgcgacatc atcttgggaa
      661 tcgctttcgt ggcgagcata acgaaaacac caccaaatgc tgctcccaaa aatgctgcaa
      721 acacataacc cctattctga gtacttacat aagacatggt attagctcct actggattga
      781 aaatcactaa cttttaccat ttttccctag acaaggaata gctacattct acaagctgaa
      841 tttcctaagg cgcgacggat tgcaccaata gcagcggatc atcactcatc aaaccatccg
      901 caccgaaaaa gcgcatagcg cgaaacatca atgggtgttc cattacacca aaccagacga
      961 aaacctgacg tccctctcta tgagcctgct gaatcatcga cggaaatatc aagaccattt
     1021 cagccatggg gcaaacaaac tgggaatcgg caggtggatt gctgatgtca aattgccaga
     1081 gaccatagag agcacagaaa tttaggtcgg ggtttaatcg atgcagtgta tctagcgagg
     1141 caggttcaaa tgattgcaca acagtctggt caacataatc tagctcttcc aattcttgca
     1201 ttaatttcgt ttcaatgcct ggatagaggt gtgctgactt tgtctctgga agtattttca
     1261 cgccgttcat tttcgctagc ctgaccacgt cctcaaaagt gggcacttgt tctccactgc
     1321 ctgcatctag tgcacgtatt tgatccaggg tgagattcct cacttcaccc attccattgg
     1381 ttgtgcggtc cacgctctca tcgtggatta caaccagttc atcatctttc gtcatttgca
     1441 catcaaattc aagccagtca acgccctgtt gtatggctgt acggaaggca gacagcgtgt
     1501 tttcgggtgc gtgtattggg cctcctcgat gagcgatgta ttgtgtcggc gtgattggct
     1561 cgctacgcaa atatgctt
//
LOCUS       DBNHOH010000944         6777 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_571804, whole genome shotgun sequence.
ACCESSION   DBNHOH010000944 DBNHOH010000000
VERSION     DBNHOH010000944.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6777)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6777)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6777
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_571804"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="AC16V7_17010"
     CDS             <1..660
                     /locus_tag="AC16V7_17010"
                     /inference="COORDINATES: protein motif:HMM:NF013539.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIR6258877.1"
                     /translation="EMQAAIRAELPSYMTAIVRMQVQLQAKQPSNDLLRSVYQADINN
                     IQRTVSNISEEKLNAALETLVNSNRILVVGAGLSSSCAMFLAHSLKIIGRDARVNSNE
                     GLSLAADIALLNPGDLLIAIDMWRYVRSTIQAVSKAKEMGVSVIAITDSIVSPLARLA
                     DYAFEVVTSGISHSLSATAIMSLLNVFIAALSYKIPEQTMRSLRRVDSAYRDNDLLVL
                     E"
     gene            663..1847
                     /locus_tag="AC16V7_17015"
     CDS             663..1847
                     /locus_tag="AC16V7_17015"
                     /inference="COORDINATES: protein motif:HMM:NF014381.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HIR6258878.1"
                     /translation="MNILICGGGNAAHALAGLLSTQEDLRVRVYLSFEDEARQWKEGL
                     AEQKSLHVHLPQGELVGRPVQVSSQAAEIVPGADLVLLALPAFAHEVVLRQMTPHLQP
                     AAWVGALPARGCFDLCALQTLGEKAPAVNIFGLQTLPWACRIERYGRSVRVLGTKAKV
                     DLASWPAELAPQIAATLSPLLGVNLQPVSSLLSLTLAGTGQLIHPGIMYGLFHNWDGQ
                     PYAQAPLFYQGIDSYTAGILQSMSDEVQELRHNLEARFSWLDLTAVRNLYDWLRFSYT
                     SDIADDTTLQSAFTSNRSYAGLQAPMRSVDGGLVPDFHARYISEDVPYALIATRGIAE
                     LAGVPTPQIDQVIRWAQGCLGQEFLVSDVLRGHDLGTSRAPQRYGYQSINSLVESTRM
                     PV"
     gene            1916..5623
                     /locus_tag="AC16V7_17020"
     CDS             1916..5623
                     /locus_tag="AC16V7_17020"
                     /inference="COORDINATES: protein motif:HMM:NF025309.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HIR6258879.1"
                     /translation="MPTRNKSTQKDTKTSQKRRVMVGAIGKCVHNLGVENFADWMQDL
                     GTGYVSVKLGPAVPIREAVNKIREARPEVVGISMRLGDLHVDKLISEFIETATQYGLS
                     PRDSGIRYSFGGLRPAANLVRAMTGLPLEEDRFIRDEERHYDLAQVAEEYKKKEHFQG
                     FFELVADDFVSMEELEGFAQRRSTVTARKDAPWSDYLVERMRQVRERENRPIIRAHIG
                     IAADTIEPTIKAIEKLADAEAFEIVSLAPDQTSQELLAKFIRGEEDPSKYLAGQGGAP
                     IRTVEDLQRLKAATQRGNYPMTRIYTGTDELVALANLWEDNLNICFPAVPIFFYNELD
                     GRGPISIRDSFDEHFRTIEHWAKIGKPLEINDPHQWGLRYASDDMQVTDHVLCAVIAL
                     KKGIKNYVMQMMFELPPEISALDDLAKMRASYELAEPLARNFDFRIIRQTRSGLPSFP
                     PNLNQAKGHLAFGIYTQLYMQPDILHVVTHSEAHHEATADDIIESCEIVKQVCWDFAK
                     GNVPKIWSDREMKMRIKELKQGAMYNFLHCAILGGYDGPIRPENFWEWAKERREDREK
                     NYETMLLSLIDEANYPTGICGVIAGDTLDLALQIGLFQGPHITVVDRRYEMAGACRTK
                     VVNGMCRTEEWNGIKVFSEFERVDLVRKTYPWYFYKEVTRADEMSFIAEDAEVEKIDE
                     DAVNEFRKQIGITGLADEKVLVVDFGSTYTKVGILDTKKETFELRYKPTTVEDIRIGL
                     ANGLGVLEACRESGDWKPLAKKMREFAVRLPCSSAKGGLKMVTVALVKEESGFAAELA
                     ALTAGAKLVASYDGMLTAEQARQIYEVDQPEIILIAGGTDEGGDSSTQLHNARLLARE
                     SRYANYTDYGVPVIYAGNQDIRDRIRNIFRYRNIHLRLTPNVMPEINDFRIEVVNEAI
                     RELFQTVIIRGKGFDVVDEYMDAPFIPTPRAAFRGINLLANGYGNEEGLGNIMALDIG
                     GATTDFYSNVNDNPLYVFPGDDAQRKVKRTILKTPNVPLVFRRVEGKFGLSYNAENLK
                     ELESFKDGSMARDLSRYLDHRFVKEFAPGEDQFRRFIHRQNGSHQVDLDGYLSWISQN
                     PHQNPETPLENAATSYLAREIMTVTTGKHVGHVDETETYFLQYGVNYFNQPVTVLLIG
                     GTIYQKCRDRESGYLDDLMTIAEGVLYNPREDTSLRPKAPVLLDAKYLVSILGGLYGR
                     VNPEQALQVMKRELLPLDRIAQESSQAVLKS"
     gene            5752..>6777
                     /locus_tag="AC16V7_17025"
     CDS             5752..>6777
                     /locus_tag="AC16V7_17025"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR6258880.1"
                     /translation="MSRKLSYFIRVLLPLAVILTACAQPPAAPPEEMIVKETVVVVET
                     VVVTEKEEVEKVITPTPVPGRQTIIVGLSQAPSEGLDPADHRSRLSETVIRNMFDGLV
                     TRDNRSGVHLELAEEMNWLDDVTLEVKLRQGVKFHDGVEMTADDVVFTFNRIIQENQI
                     EYPEPHTSPRKGLIAPLASIEKVDDYTVVMNFSDPWPPAMQGIVHQQIVPKHYLEEVG
                     TEGFVANPIGTGPFKFVSAQPGFEEVVLERFDDYYGGAPDLEPVGPACVSGVIFRTIP
                     EASTRVAALLAGEVDIIQSVPAELIDTLAQTPGIQVKTAAGTQPKWMEMNVNKPPFDD
                     VRVRQAMN"
BASE COUNT         1713 a         1796 c         1765 g         1503 t
ORIGIN      
        1 gagatgcaag ccgcgatacg agccgagttg cccagttaca tgacagcaat tgtacgcatg
       61 caggttcaat tgcaagcaaa acagccatcc aatgacctgc tccgatccgt atatcaagcc
      121 gatatcaata acattcagcg tacagtaagc aatatttccg aagagaaact gaatgccgcg
      181 ctagaaacac tggtgaattc caaccgtatt ctggttgtgg gtgcaggcct ctcgtcatcg
      241 tgcgcgatgt tcctggctca ttcgctaaaa attatcggtc gagatgcacg tgttaactca
      301 aacgaaggtc tttcgctagc tgctgatatc gccctcctaa atcctggtga cctgctgatc
      361 gccatcgata tgtggcggta cgtgcgctca accatccagg ctgtcagcaa agctaaggaa
      421 atgggcgtca gcgtcatcgc catcacagat agcattgttt ccccgcttgc ccgcctggca
      481 gactatgctt ttgaagtcgt aaccagtggt atttcacaca gcctatccgc aaccgccatc
      541 atgtcgttgc tgaacgtctt tatcgctgcc ctttcctata aaattcccga acaaaccatg
      601 cgttcactaa ggcgcgttga cagcgcttac cgcgataacg atctattggt gttggagtga
      661 gcatgaacat attaatctgt gggggtggaa acgccgccca cgccctcgca ggcttgctct
      721 caacacaaga agacctgcga gtcagggttt acctgtcatt tgaagatgaa gcccgccagt
      781 ggaaagaggg cctcgcagag cagaaaagcc tccatgttca cctgcctcag ggggagctag
      841 tgggccgacc tgtccaggta agctcccaag ctgctgagat tgtccccggc gccgatctgg
      901 tattgttagc tttgcctgcc ttcgcccatg aggtcgtttt acgccagatg acgccacatc
      961 tccaacctgc tgcctgggta ggggcactac cagctcgtgg ttgttttgac ttgtgcgccc
     1021 tgcaaacatt aggagaaaaa gcacccgcag taaacatctt cggtcttcaa acactacctt
     1081 gggcttgccg gatcgaacgc tacgggcgat ctgtgcgtgt gttgggaacc aaagcaaagg
     1141 tggatttggc atcctggcca gcagagctgg ctccccagat tgccgctacg ctaagccctc
     1201 tgctaggagt aaatttacag cccgtttcga gcctactaag cttaaccctg gcaggtaccg
     1261 ggcagttgat ccacccagga attatgtatg gcttattcca caattgggat gggcaaccat
     1321 atgctcaagc accacttttc tatcaaggca ttgactctta taccgctggg attctgcaat
     1381 ctatgagcga cgaagtgcaa gaactgcgtc acaatttgga ggcgcgcttt tcgtggttgg
     1441 atcttacagc cgtgcgcaat ctttatgact ggctgcgttt ttcttacacc agtgatattg
     1501 cggacgatac aaccttgcaa tcggcattta caagcaatcg cagctatgca ggtctgcagg
     1561 cccctatgcg atctgtggat ggcggactgg tgcccgattt ccacgcccgc tacatctcag
     1621 aagatgtccc ctatgcactc atcgccacgc gaggtattgc tgaattagcg ggtgttccca
     1681 ctccacaaat tgaccaggtg atccgttggg ctcaagggtg tttgggacag gaattcctgg
     1741 tttcggatgt gctacgcggt cacgacctgg gcacttcccg cgccccgcag cgttacggtt
     1801 atcaatccat caattcatta gtggaaagca cccgaatgcc tgtctgaaca gcaacacatt
     1861 tgctgaatcg acgaatcatg taaggcgaat aaacatcatt tctggagaaa tatatatgcc
     1921 aactcggaat aaatcgaccc aaaaagacac caagacctcc caaaaaaggc gcgtcatggt
     1981 cggcgcaatt gggaaatgtg ttcacaacct gggcgttgag aattttgccg attggatgca
     2041 ggacctaggc accggctatg tctcggtaaa acttggccca gcggtcccga tccgggaggc
     2101 agttaacaaa atacgcgaag cgcgcccgga ggtggtgggc atctcaatgc gcctcggaga
     2161 cctgcatgtt gataagttga tcagcgaatt catcgagacc gcaactcaat acggacttag
     2221 cccacgcgac tccggtattc gatacagctt tggcgggctg cgaccagctg cgaatctggt
     2281 gcgggcgatg accggcctgc ctttggaaga agaccgcttc atccgagatg aagaaaggca
     2341 ctatgacctg gcccaggtag cagaggagta caagaagaaa gagcacttcc agggtttctt
     2401 tgagcttgtg gcagacgatt tcgtctccat ggaagagctg gagggctttg cacaacggcg
     2461 ttccactgtc accgctcgta aggacgcccc gtggtctgac tatttagtgg agcgcatgcg
     2521 acaagtgcgt gagcgcgaaa accgtccgat tatccgtgcc cacattggca tcgctgccga
     2581 caccattgaa ccaaccatca aagcgattga gaaacttgcc gatgctgaag ccttcgagat
     2641 tgtgtcgctc gcgcctgacc agacatcaca agaattgctc gcaaagttca tccgcggcga
     2701 agaagacccc agtaaatacc tggctggtca gggaggcgcc cctatacgta cagtggagga
     2761 tctccagcgc ttaaaggcgg ccacccaacg tggcaattac cctatgaccc ggatttacac
     2821 cggcacggat gagctcgtag ccctagcgaa tctttgggag gataatctta atatctgctt
     2881 cccggctgtt cctatcttct tctataacga gttagatggg cgtggaccga taagcatccg
     2941 cgactcattc gacgagcatt tccgtactat tgagcactgg gccaagattg gcaagccctt
     3001 ggagatcaat gacccccacc aatggggttt gcgatatgcc agcgatgaca tgcaagtcac
     3061 ggatcatgtg ctctgtgcgg tgatcgccct caagaagggc attaagaatt atgtgatgca
     3121 gatgatgttc gagctcccac cagaaatatc tgccttggat gatctggcga aaatgagagc
     3181 ctcatacgag ctagctgagc cactcgcccg caactttgat ttccgcatca tccgccagac
     3241 gcgctctggt ctgcctagct ttccacctaa cctgaaccag gccaaaggtc atctcgcttt
     3301 cggcatctac acccagcttt acatgcagcc tgatattttg catgtcgtca cccactctga
     3361 agctcaccac gaagcgacag cagacgacat cattgaatcc tgcgaaattg tgaagcaagt
     3421 atgctgggat ttcgccaaag gcaatgtgcc aaagatttgg tctgaccgcg aaatgaagat
     3481 gcgcatcaaa gagctcaagc agggcgctat gtacaacttc ctgcactgcg ccatcttagg
     3541 tgggtacgat ggccccatcc gtcctgaaaa tttctgggag tgggcaaaag agcgccggga
     3601 agatcgggaa aagaattacg aaacaatgct gctcagcctg atcgatgagg ccaactatcc
     3661 caccggaata tgtggtgtga tcgcaggaga tacacttgac ctggcacttc aaatcggcct
     3721 cttccaggga ccgcatatca ccgtggtaga tcgtcgctat gagatggcgg gagcctgccg
     3781 aaccaaggtt gtgaatggga tgtgccgcac cgaggaatgg aacggcatca aggtctttag
     3841 tgaatttgaa agggtcgacc tggtccgcaa aacctatcct tggtatttct acaaagaagt
     3901 gacccgcgcc gatgaaatga gcttcatcgc ggaagacgct gaagtagaaa agatagatga
     3961 agacgcggtg aacgaattcc gcaaacagat tggaatcaca ggtctagccg atgagaaagt
     4021 gctggtggtg gatttcggca gcacttacac caaggtcggt atcttggata ccaaaaaaga
     4081 gaccttcgag ctgcgttaca agcccacaac cgtggaagac atccgtatcg gactagccaa
     4141 cggcttggga gtactagaag cctgtcgaga gagtggcgat tggaagcccc ttgcgaagaa
     4201 aatgcgggaa ttcgccgtac gattgccatg ctcgagtgcc aaaggcggcc taaaaatggt
     4261 tacggtggcg ctggtaaagg aagaatcggg atttgcggct gagttggcag cccttaccgc
     4321 tggcgcgaag cttgtcgcat cctatgatgg gatgttgact gctgagcaag ctcgccaaat
     4381 ctacgaagtg gaccaaccgg agatcatcct catcgcgggc ggcactgatg aaggcgggga
     4441 ttcatcgact caactgcaca atgcccgtct cttggctcgt gaatctcgtt acgctaacta
     4501 cactgattac ggtgtgccgg tcatatatgc tggcaaccaa gacatccgcg atcgcatccg
     4561 caacattttt cgctaccgca acatacacct tcgcctgaca cccaacgtca tgccggagat
     4621 caatgatttt cgcatcgaag tagttaatga agccatccgc gaactcttcc agactgtgat
     4681 cattcgcggc aaaggttttg acgtggtgga tgagtacatg gatgccccat tcatccccac
     4741 cccgcgcgcc gctttccgtg gcatcaatct gcttgcgaat ggctatggca atgaagaagg
     4801 gttgggcaac ataatggcac tggatatcgg tggtgctact accgatttct actcaaatgt
     4861 gaacgacaac ccactatacg tatttcccgg agatgacgca caacgaaagg tgaaacgcac
     4921 catccttaaa acgcccaatg tgccgcttgt cttccggcgg gtggaaggta aatttggctt
     4981 gagttataac gctgaaaatc taaaagagct ggagagtttc aaagatgggt ctatggcgcg
     5041 cgatcttagc cgctacttag atcaccgctt tgtcaaggaa tttgcacctg gcgaagatca
     5101 attcagacgt ttcatccacc gccagaacgg ctcccatcaa gtagacctgg atggctacct
     5161 gagttggatc agccagaatc cgcaccagaa tccggaaacc ccgctggaga acgcagccac
     5221 ctcgtacctg gcgcgagaaa ttatgaccgt cacgactggg aagcacgttg gtcacgtaga
     5281 tgagaccgag acatacttct tgcaatatgg ggttaactat ttcaaccaac cagtgactgt
     5341 gctgctgatc ggcgggacaa tctaccaaaa atgtcgcgat cgcgagtcag ggtacctgga
     5401 tgacctgatg acaatcgctg aaggtgtgct ctataatcca cgggaagata cctcgctgcg
     5461 cccgaaagcc ccggtgctgc tggatgccaa gtacctggta agcatcctgg gtggtctata
     5521 tggacgtgtg aatcccgaac aggcattgca agttatgaaa cgcgagctcc ttccgctcga
     5581 taggatagcg caggaatcgt cacaggctgt tcttaaaagc tgaacattgt gtgtcacagt
     5641 atatcaaaaa gcatacaggt aatatatgaa aggaggcacc tgggtaaccg caatatgaac
     5701 caaagccacc aaaaattcaa tcaataaatt ctatttatca ggaggagtaa aatgtcacgt
     5761 aagttaagtt attttatccg tgtcctattg ccgctggcag tcatcctgac cgcctgcgcc
     5821 caaccacccg cggcaccacc agaagagatg atcgttaaag agacggtcgt agtcgtagag
     5881 acagttgtgg tgaccgaaaa agaggaggtg gagaaagtca tcacccccac acccgtgccc
     5941 ggtcgccaga ccatcatcgt ggggctttcc caggctccca gcgaaggact ggatccggcc
     6001 gatcaccgca gtcgcctgtc cgaaactgtc atccgcaata tgtttgacgg actcgtcact
     6061 cgcgataaca ggagcggcgt acacctggag ctggctgagg agatgaactg gctggatgat
     6121 gtcaccctgg aagtcaaact gcgccaaggc gtgaaattcc acgacggtgt ggagatgacc
     6181 gccgacgatg tggtcttcac cttcaataga atcatccagg aaaaccagat cgaataccct
     6241 gagccccaca cctccccgcg caagggcttg attgcacccc tggcttcaat tgaaaaggtg
     6301 gatgactaca ccgtcgtcat gaacttcagc gatccctggc cgcctgccat gcagggcatt
     6361 gtccaccagc aaattgtacc caagcactac cttgaagaag tggggactga aggttttgtc
     6421 gccaatccca tcggtacagg acctttcaag ttcgtctccg cacaacctgg atttgaagag
     6481 gtcgtcctgg aaaggttcga tgattactac ggcggagcac ctgaccttga gccagttggg
     6541 ccagcctgtg tcagtggtgt aatattccgt accataccag aagcctccac ccgtgtggcg
     6601 gccttgttgg ctggcgaggt cgacatcatc cagtccgtcc cggctgagct gattgacacc
     6661 ctagcccaga caccgggcat ccaggtcaag actgcagctg gcactcagcc gaaatggatg
     6721 gaaatgaacg tcaacaagcc acccttcgat gatgtgcgcg tgcgccaagc gatgaac
//
LOCUS       DBNHOH010000945         2375 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_123862, whole genome shotgun sequence.
ACCESSION   DBNHOH010000945 DBNHOH010000000
VERSION     DBNHOH010000945.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_123862"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="AC16V7_17030"
     CDS             <1..415
                     /locus_tag="AC16V7_17030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR6258881.1"
                     /translation="AENYYGDRFVIVGDAAGLVRAFKGKGITSGVSTGIRAARTMIEH
                     GISRAAFHEHYRAANQDITGDLSYGRFVRLLAINMGRIGWMDAVICAAQNDERLQCAL
                     YDAVSAVGPYKDVLKNAMNPTSVLGVLKHLFTRRA"
     gene            478..1242
                     /locus_tag="AC16V7_17035"
     CDS             478..1242
                     /locus_tag="AC16V7_17035"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HIR6258882.1"
                     /translation="MNAIRGVIFDIDGVLIFQGRVYPNAIEVVDTLRAKGLVLRFLTN
                     STLNSRASCTEKLRQSGFHVLEDEVITASYATAVYLRSQNPRSCWVMVDREGIQEFKE
                     FVQDTDNPEYIVIGDNRSQFNFDHLNKVLRHLVNGARLIAMQSELIDTSMGNIELNVG
                     SWVGMLERAAGVRAVSIGKPNPYVFELALHSMHLDKNDLIMVGDRITTDIQGANYFGL
                     RSVLIKTGEFDERDLASEIVPTYTFETIGELLELFS"
     gene            complement(1406..>2375)
                     /locus_tag="AC16V7_17040"
     CDS             complement(1406..>2375)
                     /locus_tag="AC16V7_17040"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6258883.1"
                     /translation="YVTSLVPIRENIVRRIEVVPGVEAVAPVRYFEIEWRRPGNDWET
                     VNFMAFSPASYTHVTSFVFSQSDSESINNILRGGHVFISSVIAEKHGLAPGDRLLIKT
                     RLGETPFTVAAVTVDFSNQGMVINGSWNDMEHYFRLRGANTLLIKVTPGTSPDAVGEE
                     IDSRYGKGENLVIISNETLLGRISTLMQQAFRMFDVLALIAMLVGFMGITNTLTMNVM
                     ERTQEIGMLRAVGMTRGQVVRMVLSESALMGLIGGLMGLVFGAVLSRLFLSAMASMSG
                     YRLTYVLPIENIFEALLIAFLTSQLAAFLPTLRASRTRILEAIQYE"
BASE COUNT          635 a          553 c          611 g          576 t
ORIGIN      
        1 agcggaaaat tattacggag atcgttttgt gattgtaggg gatgctgcgg gcttagtgcg
       61 cgcctttaag ggcaaaggga tcacatcggg tgtatcgacg ggcatacggg ctgcaaggac
      121 gatgatagaa catggcatct ccagggctgc ctttcatgaa cattatcgtg cggcaaatca
      181 ggatatcacc ggagacttgt cttacggacg gtttgtccgc ttattagcca tcaacatggg
      241 acggattggt tggatggacg ccgtgatttg cgctgcccag aacgatgagc gtctacaatg
      301 cgctctatat gatgctgtct cagctgttgg cccttacaag gatgtcctca aaaacgccat
      361 gaatcccacc agcgtgcttg gggtcctcaa gcatctattt accagacgcg catgacctag
      421 cggcgcatca ggggggtgta caggaatgtg cggacaggca agcaaggaag tttaggtgtg
      481 aatgctattc ggggtgtgat ctttgatata gacggcgtgc tgatctttca gggcagggtt
      541 tatccaaatg caatcgaggt ggtagacaca cttcgagcga aaggcctcgt cttgcgattc
      601 ctgaccaaca gcaccttgaa cagccgagcc tcttgtaccg agaaattgag gcaatctggg
      661 ttccatgttc ttgaggatga ggtgatcacc gcttcatatg ctaccgcagt ctacttgaga
      721 tcacaaaatc caagatcatg ctgggtgatg gtagatcgag aaggtattca agaattcaaa
      781 gaatttgtcc aagacaccga taacccggag tacattgtga ttggagacaa ccgtagccaa
      841 tttaattttg atcatctgaa taaagtcttg agacatctgg tgaacggtgc gaggctgatc
      901 gccatgcaat ccgagctgat cgataccagc atgggcaata tcgagttaaa cgtaggttcg
      961 tgggtgggga tgctggaaag ggctgcggga gtacgagcgg tcagcattgg caagcccaac
     1021 ccatatgttt ttgaactggc attacacagc atgcatttag acaaaaacga cctcatcatg
     1081 gttggggatc gcatcactac agatatacag ggggcaaatt attttggtct tcgctctgtg
     1141 ctgattaaga ctggcgaatt cgacgagcgg gacttagcca gcgagattgt gcctacttac
     1201 acctttgaaa ccataggtga attgctagag ttgtttagct aatcgaaaaa ccttaccaaa
     1261 ataacagaga tccgaaagaa aaaagcgcct caaagggcac tttttttact gagggattta
     1321 gattctcctg ccttgagtaa tattgaaata aataattgtt gtggaggtat tgggtatagc
     1381 ctctttgact atataatgct ctgcctcact cgtattgaat ggcttccaag atacgagtcc
     1441 gtgaggcgcg taaagtgggc aggaatgctg ccagttggga ggtcaggaaa gcgatcaata
     1501 aagcctcaaa gatgttttct atcggcagta cataggtcag acggtagcca gacatgctgg
     1561 ccatcgcgct aaggaaaagt cgggaaagga cagcgccaaa caccaagccc atcaatccgc
     1621 ctatcaagcc cattaatgct gactcagaca agaccatgcg cactacctga ccacgcgtca
     1681 tgccgactgc gcgcaacata ccaatctcct gcgtgcgctc catgacattc atggtgagcg
     1741 tatttgtgat ccccataaag cctaccagca ttgcaattaa tgcaagcacg tcaaacatac
     1801 ggaatgcctg ctgcatgagg gtggaaattc gtcccaatag agtctcgttt gagattatca
     1861 ccagattttc tccttttcca tagcgggaat caatctcctc gcccactgcg tcaggggaag
     1921 tgcctggtgt tacctttatc agcagcgtgt ttgccccacg tagccggaag tagtgctcca
     1981 tatcattcca tgatccattg ataaccatac cctggttaga aaaatcaact gtgacagctg
     2041 cgacggtgaa aggtgtttcc cccaggcggg tcttaatcag cagacggtca ccgggtgcca
     2101 ggccatgctt ctcggcgatc acactggaga tgaagacatg cccgccacgc agtatattat
     2161 ttatagattc tgagtcggat tggctaaaaa caaagctggt gacatgtgta taagaggctg
     2221 gggaaaatgc cataaagttg accgtttccc agtcattacc aggccgccgc cactcgatct
     2281 caaaataacg cactggtgcg actgcctcca cccctggcac cacctcaatg cgccggacga
     2341 tattctctcg aatgggaacc aaagatgtca catat
//
LOCUS       DBNHOH010000946         2778 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1149582, whole genome shotgun sequence.
ACCESSION   DBNHOH010000946 DBNHOH010000000
VERSION     DBNHOH010000946.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1149582"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="AC16V7_17045"
     CDS             complement(<1..250)
                     /locus_tag="AC16V7_17045"
                     /inference="COORDINATES: protein motif:HMM:NF017015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR6258884.1"
                     /translation="MFSKEMIQTLYGYHYWANDRVLDYAARLEPADYFADAGYSHGSL
                     HGILFHILRTDYAWRISCQTLKPAVEPLLLENFPDLQAI"
     gene            complement(251..1981)
                     /locus_tag="AC16V7_17050"
     CDS             complement(251..1981)
                     /locus_tag="AC16V7_17050"
                     /inference="COORDINATES: protein motif:HMM:NF012239.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HIR6258885.1"
                     /translation="MRILNEKQEALLANERGLLNDLRVALVQFGAASEDHETLGQSIR
                     QLDELFLLVVVGEFNSGKSAFINALLGQKVLQEGVTPTTTQINILRYAEAPGRLYDNE
                     NVQTLTAPVDMLTEISIVDTPGTNAIIREHEAITTQFVPRSDLVLFITSADRPFTESE
                     RLFLQRIRDWGKKVVVVLNKIDLFQSDDELAQVQSFIAENARTLFGLTPEIFPVSARL
                     ALRAKMGEPSLWATSRFEPLERYIHDTLDEKGRLHLKFLSPLGVGLHLSEKYLGVIDS
                     RLDLLKDDFTTLEDVEAQLSVYKEDMQRDFNYRMSDIEKVLFEMEQRGQDYFDETLRL
                     GRVFDLVNKSKIQREFEGHVVGDTPQRIEAKTGEIIDWLVDSDLRQWQAVNEHLAERR
                     RQHAERIVGDSAGGTFHHDRERLIEGVGREARRVVDSYDKTQEAQAIAEGARNAVAAL
                     AAVEVGALGLGTLVTVLATTATADVTGVLLASLMAALGLFLIPAKKRQAKLEMRTKVG
                     AMREQLARSLRVQFEREIERSLGNIHEAIAPYTRFVRAEQGKLQGAQQELEKIKTGLG
                     KLKVEVEQGD"
     gene            complement(2038..>2778)
                     /locus_tag="AC16V7_17055"
     CDS             complement(2038..>2778)
                     /locus_tag="AC16V7_17055"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR6258886.1"
                     /translation="GGGVRGGGFSSMPTPESARSWYPASDPVHGFDHVLRVFHLAEQL
                     AQAEGAELEIVRAAALLHDATGDQRSVDNHGLTYAQAGGQPSEISNQHSTINNIRLNH
                     HHASSDFAAQVLRAEGWDEARIAAVQHCIRAHRFRDDREPPHTLEAKILFDADKLDAI
                     GAVGVARAIGYATQAGQPAYAKPSQQFLDSGELEAGEPHSAYHEFLFKLRHLKDRLYT
                     PAAKSIANERHRVMAEFFERLAGEMGGK"
BASE COUNT          556 a          823 c          785 g          614 t
ORIGIN      
        1 ggatagcttg caggtcgggg aaattttcca acaaaagtgg ctcgacagcc ggtttgagtg
       61 tctggcagct gatccgccag gcataatcgg tgcgcagaat gtgaaaaaga atgccgtgca
      121 agctgccatg gctgtagccc gcgtcagcga aataatcggc tggctctagc ctggcagcgt
      181 aatctaagac gcgatcatta gcccagtagt ggtagccata caacgtttgg atcatctctt
      241 tggaaaacat ttaatctccc tgctctacct ccaccttcaa tttgcctaag ccggtcttga
      301 tcttctccaa ctcctgttgg gcaccctgca gcttgccctg ctcagcgcgc acaaagcggg
      361 tataaggggc gatggcttca tggatattgc ccaggctgcg ctcgatctcg cgctcaaact
      421 gcactcgcaa ggaacgagca agttgttcgc gcatggcgcc gaccttggtg cgcatctcta
      481 gcttggcctg acgctttttg gcagggatca agaacaagcc cagcgcagcc atcaagctgg
      541 ccaataacac gccagtcaca tcggcggtag ccgtggtcgc caggaccgtc accagcgtcc
      601 ccaacccaag ggcacccacc tccacggctg ccagggcagc caccgcgttg cgtgccccct
      661 cggcaatggc ctgggcttct tgggtcttat cgtagctgtc cacaacccgg cgcgcctcgc
      721 ggcctacccc ttcgatcaaa cgctctcggt cgtggtggaa cgtgcccccc gcagagtctc
      781 ccacaatgcg ctcggcatgc tggcgccggc gctctgccag atgctcgttc accgcctgcc
      841 actggcgtag atcggaatca actaaccaat cgatgatctc gccggttttg gcctcgatgc
      901 gctgaggggt gtcacctacc acatgcccct caaattcacg ttgaatcttg ctcttattga
      961 ctaaatcgaa gacacgcccc aggcgtagtg tctcgtcgaa ataatcctgg ccgcgctgtt
     1021 ccatctcgaa gagcaccttc tcgatgtcag acatgcgata gttgaaatcg cgctgcatgt
     1081 cttccttata gaccgaaagt tgagcttcca cgtcttctaa ggtggtgaag tcatctttga
     1141 gcaggtccag ccgggagtcg atcacgccca gatatttctc tgacaagtgc agccccacgc
     1201 ccagtgggct gaggaacttc aggtgcaggc ggcccttctc gtcgagtgta tcgtgaatgt
     1261 agcgctctag cggctcaaat cggctggtag cccacaggga gggctcgccc atcttggcgc
     1321 gcagtgccag gcgggcgctg actgggaaga tctccggtgt caggccaaat aaggtgcggg
     1381 cattttcggc tatgaagctc tgcacctgcg ccagctcatc atcgctttgg aataaatcga
     1441 tcttattaag caccactaca accttctttc cccaatcgcg aatgcgctgc aggaaaagtc
     1501 gctcgctttc ggtgaagggt cgatcggccg aggtgatgaa taggaccaaa tcggagcgcg
     1561 gcacaaactg ggtggttatg gcttcatgct cgcggatgat ggcgttggtg cccggcgtgt
     1621 ccacaatgct gatctcagtc agcatatcca cgggagcggt cagggtttgc acgttctcat
     1681 tgtcgtacaa gcgcccaggc gcctcggcgt agcgcagtat attgatctgc gtggtggtcg
     1741 gcgtaacacc ttcctggagc actttttgcc ctagcagagc gttgatgaaa gcgctcttgc
     1801 ccgagttgaa ctcccctacc accaccagca ggaatagctc atcgagctgg cgaatggact
     1861 gccccagtgt ctcgtggtct tcggatgccg ccccaaactg gacgagggcg acgcgcaggt
     1921 cgttgagcag gccgcgctca ttcgctagta gggcttcttg tttttcgttg aggatgcgca
     1981 tgtgtgatat tccctggcaa aaattatagc attagtaagg ggagaatcga ttttgcctta
     2041 cttacccccc atctcccctg ccagccgctc gaaaaattcc gccatgaccc ggtggcgctc
     2101 attagcgatg gacttcgccg caggcgtgta cagccggtct ttcagatggc gtaacttgaa
     2161 gagaaactcg tggtaagcgc tgtgcggctc gccagcctcc agttccccgc tatccaagaa
     2221 ctgctgggat ggcttggcat aggcaggctg accagcctgc gtggcgtatc caatggcgcg
     2281 cgccacaccc accgcgccga ttgcgtccag cttatccgcg tcgaaaagga tcttggcttc
     2341 tagggtgtgt ggcggctcgc gatcatcgcg gaagcggtga gcgcggatgc agtgctgcac
     2401 ggcggcgata cgcgcttcat cccagccttc cgcccgtaaa acctgcgcag cgaagtcgga
     2461 tgaagcgtgg tgatgattca atcttatatt gttgattgta gaatgttgat tgctgatttc
     2521 tgacggctga ccgcctgcct gggcgtacgt caggccatgg ttgtccactg accgctgatc
     2581 tcctgttgca tcatgtagca gcgcggcggc gcgcacgatc tccagttcag cgccttcggc
     2641 ctgggctaat tgctccgcca ggtggaagac gcgcagcacg tgatcgaacc cgtgcacggg
     2701 gtcagaggcg gggtaccagg agcgggcgga ttcaggagta ggcatagaac taaacccccc
     2761 acccctgacc cctccccc
//
LOCUS       DBNHOH010000947         5365 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1471235, whole genome shotgun sequence.
ACCESSION   DBNHOH010000947 DBNHOH010000000
VERSION     DBNHOH010000947.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5365)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5365)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5365
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1471235"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            384..1682
                     /locus_tag="AC16V7_17060"
     CDS             384..1682
                     /locus_tag="AC16V7_17060"
                     /inference="COORDINATES: protein motif:HMM:NF019912.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LVIVD repeat-containing protein"
                     /protein_id="HIR6258887.1"
                     /translation="MKKLTVVLSLALICMLTLVGIASAGHDDDPHTQNLHAMGHDEAP
                     ASLIYGPTDVHTDIAFWGKYAIQGNWNGFRVLDISAPGNPKLLNFVECQGDQGDVSVW
                     GNLVFRSTNSPNPGSRSCGGEAVPVGFEGIHIFDISDLSAPALIATVPTTCGSHTHTL
                     VPDTGNNRVLLYVNSSNSTCQSGTVSGAVIDIIEVPLDAPESASIIHSEPLLDGALFC
                     HDACVILGDANLLACATSPVVNVFDISNPADPEPLYSIAEPGVTIPWHSAAFTWDGEV
                     IVLGWEPGGGVAPSCEANDPDVFKSFFFYDADDGAKLGQWTLPRAQSALENCTLHNFN
                     VVPLRSGRYVVAHGSYQSGTSVVDFTDPANPYEVAYSDPPAIDPLDIGGAWSSYWYNN
                     FIYESNITEGLNVFRLSDRATAGAMRLPYLNPQTQDFTLP"
     gene            complement(1830..2048)
                     /locus_tag="AC16V7_17065"
     CDS             complement(1830..2048)
                     /locus_tag="AC16V7_17065"
                     /inference="COORDINATES: protein motif:HMM:NF013224.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HIR6258888.1"
                     /translation="MLITIRTGDLRNVVIQLRRVEGVIEANMTFGPYDAVAIIQADDV
                     KHLGNIIAMGIQPIPGVLDTLTCLSVEL"
     gene            complement(2101..2946)
                     /locus_tag="AC16V7_17070"
     CDS             complement(2101..2946)
                     /locus_tag="AC16V7_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HIR6258889.1"
                     /translation="MNADIRIDALLPPEMAKRAEYLGVRKAEAKISAMFSLAVLAGIF
                     IALGAMFATTVSAGTSGVLPFGVTRLLVGLVFCLGLILVVVGGAELFTGNNLIVMAWA
                     GGKVSTSAMLRNWVVVYLGNFVGSIATVILVIFSKQYAFGGGAVGQSMLNIAIGKVNF
                     GFFQALALGVLCNMLVCLAVWMTFSARSTIDKIMAVIFPISAFVAGGFEHSVANMYFI
                     PLGLFLKAFDPAFTASTQINVSDLTWSAFLLNNLLPVTIGNIIGGGVLVAAVYWAVFL
                     RHSEV"
     gene            complement(2970..5198)
                     /locus_tag="AC16V7_17075"
     CDS             complement(2970..5198)
                     /locus_tag="AC16V7_17075"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR6258890.1"
                     /translation="MKPDVKQFQATLGSKVFTFETGKLAGQAGGSVTLRLGDTVVLAA
                     ATMSADVRPGVDFVPLTVDYEERMYAGGRIPGSFFRREGRPTEEAILTARLTDRPLRP
                     LFPKNLRNDIQVILYSLSTDGENPIDILAVNAASAAVLLAGLPWAGPVGAVRVGWIEG
                     DFILNPTFADLEYSDLDLRMAGTRDAILMVECGASAVSEEIMVEALEFGHKAMQPLID
                     AQERIVAEAGVTKREYISAPANEDLAVTVQERAGAQLNHILQQPYIKSERNEAIDALK
                     TSLLEDLAGEDESQAPLVKEAFDQLLKTVVRQTILEHNQRPDGRGLNDIRDIWCEVDV
                     SPRAHGSGLFTRGETQVLTLATLGTPREAQELDSLSPSESKRYIHHYNFPPFSTGEVR
                     PLRGASRRDIGHGALAERALVPVIPLEADFPYTLRLVSEVLSSNGSSSMASVCGSTLA
                     LMDTGVPIKAPVAGVAMGLIKEGERYAILTDILGAEDHYGDMDFKVAGTQAGITALQM
                     DIKIKGINTTIMRQALEQARQARLYILNKMMEVIPAPNPTLKPHAPRITVVQIPVEKI
                     GAVIGPGGKTIRSIQEETGAKIDIGEDGSVFIATADGEAARRAVERVEAITETPVIGR
                     IYTGRVVRTTEFGAFVEILPGTDGMVHISQLDTERVNKVEDIVRVGDELTVMITDIDP
                     QGKIRLSRQAVLEGWTPEEAKMRDSRRTGGGGRPSYGGGRPGGRGGDRGDRGRSGGSN
                     RR"
BASE COUNT         1148 a         1535 c         1490 g         1192 t
ORIGIN      
        1 tgtggcggct tgtgccagat tggaggcgcc tggtcttctt actggtataa caacttcatc
       61 tatgagagcg atatcaccgc cggtctgaac gtcttcgggt tgagcgataa agcgcgggct
      121 ggggcaatac gcttgccgcg cctgaatccg cagactcagg aattctcact tccatagtag
      181 cccgcgacct ttcgaaaggg tggtcatctc tcatggccac ccttttcatc tggtactcgc
      241 atacacgatc tgtgttgcaa ttctgccatg taaaggtggt gattgaccgg caaaattcag
      301 atttccactg cctaaaaacc gggcgacttg cccgggttaa atcagttcga atatagactt
      361 agcacagaaa tgaggtaaat cacatgaaaa aacttactgt tgttctcagc cttgccttga
      421 tatgtatgct cactctggtt ggcatcgcca gcgcagggca cgatgatgat ccgcataccc
      481 agaacctgca tgctatgggg catgacgagg cgcctgccag tctgatctac ggccctacag
      541 acgtacacac tgatatcgcc ttctggggta agtatgccat ccagggcaac tggaatggct
      601 tccgcgtcct ggatatctcc gcaccgggta atcccaaact gctcaacttc gttgagtgcc
      661 agggtgatca aggcgatgtc tccgtatggg gcaatctcgt cttccgctct accaattcac
      721 ccaacccggg cagcaggtct tgcggcggtg aagccgtccc cgtgggcttc gagggcatcc
      781 atatattcga tatcagcgac ctgtccgcgc cagctctgat cgccaccgtg ccgaccacct
      841 gtggttcgca cacccatacc ctggtccccg atacgggtaa taaccgggtg ctgctctacg
      901 ttaattcttc gaacagtact tgccagagtg ggacggtatc tggcgctgtg atcgatatca
      961 tcgaggtgcc gttggatgcc ccagaaagcg ccagcatcat ccattcagaa ccgctcttag
     1021 atggggcatt gttctgccat gatgcatgtg tgattctggg tgatgccaat ttgcttgctt
     1081 gcgccaccag tccagtcgtg aatgtcttcg atatcagcaa cccggctgac ccagagccgc
     1141 tgtacagcat cgccgagccc ggcgttacta tcccttggca ctcggcggcc ttcacctggg
     1201 atggcgaagt catcgtactc ggctgggagc caggcggtgg tgtggcgcca tcctgtgagg
     1261 cgaacgaccc ggatgtcttc aagagctttt tcttctacga cgctgatgac ggtgccaaac
     1321 ttggtcaatg gacgctgccg agagcgcagt cggctctgga aaactgcacg cttcacaact
     1381 tcaacgtggt gccgctgcgt agcgggcgct acgtggttgc ccatggcagt taccagtccg
     1441 gcaccagtgt ggtcgatttc actgacccgg ctaaccctta tgaggtggct tactcagacc
     1501 cgccagccat cgatccctta gacattggtg gtgcctggtc gagctactgg tacaacaatt
     1561 tcatatatga gagcaacatc accgagggct tgaacgtctt ccggctgagc gacagggcga
     1621 cggctggggc catgcggctg ccatatctca acccgcagac gcaggatttc accttgccct
     1681 agtaattcag gacattcaga aagggtggtc atctcttggc cacccttttt gattcgcccg
     1741 tgtaatcttt caggttaaaa aacggatagg tgggcgagcg aaacccgtcc atctatccgg
     1801 tgatctccct ggctatttaa ctgatatggt tacaattcca ccgataggca ggtgagtgta
     1861 tccagtaccc ccgggatggg ttggatgccc atggcgataa tattccccag gtgtttcaca
     1921 tcatcggcct gaatgatggc taccgcgtca tatggaccga aggtcatgtt ggcctcgatg
     1981 acaccctcca cgcggcgaag ctggatgacg acattccgca gatcgccggt gcgaattgta
     2041 atcagcacgt aagctctcat attttcctcg tttaatttat tgtctgatac agattccggt
     2101 tcaaacctcg ctatggcgca ggaagacagc ccagtacacc gcggccacca gcactccccc
     2161 gccgatgatg ttaccgatgg tcaccggcaa aaggttgttc agcaggaacg cgctccaggt
     2221 caggtcgctg acgttgatct gcgtggaggc ggtgaaggcc gggtcaaagg ctttgaggaa
     2281 caagccaagc gggataaagt acatgttggc aacgctgtgc tcgaacccac ctgccacgaa
     2341 ggctgagatg gggaaaatca ccgccataat cttgtcgatc gtgctgcggg cgctgaaggt
     2401 catccagacc gccaggcaga cgagcatgtt gcacaagact cccagtgcca aagcctggaa
     2461 gaagccaaag tttaccttcc caatggcgat gttgagcatg ctctggccga ctgcccctcc
     2521 accgaaggca tattgcttgc tgaagatgac caggatgact gtggcaatcg agccaacgaa
     2581 gttgcccagg tacacaacta cccagttgcg cagcatggca gatgtgctca ccttgccacc
     2641 tgcccaggcc atcacgatca ggttgttgcc ggtaaagagc tcagctccac ccaccactac
     2701 caggatgagc cccaggcaga acaccagacc aaccaaaagg cgtgttaccc caaaaggtaa
     2761 cacgccggat gtgcctgccg aaaccgtggt ggcgaacatt gctccgaggg caataaatat
     2821 cccagccagc acagccaggg aaaacatggc cgagatttta gcctcggcct tacggacccc
     2881 tagatattcg gcccttttgg ccatttctgg gggaagaaga gcatcaattc gaatgtcagc
     2941 attcatattg cgtctctcct aagcaaaaat tatcttctgt ttgaaccccc tgagcgcccg
     3001 cgatcgccac ggtcgccgcc tctaccaccc ggacgaccac ctccatatga ggggcgacca
     3061 cccccgccag tgcgccggct atcgcgcatt ttggcttcct cgggcgtcca gccttccagc
     3121 acagcctgcc tggagaggcg gattttgccc tgcgggtcga tgtcggtgat catcacggtg
     3181 agctcatcgc ccacgcgaac gatgtcttcc actttattga cgcgctctgt atcaagctgg
     3241 gagatgtgca ccatgccgtc ggtgcccggc aggatttcaa cgaaggcgcc gaactcggtg
     3301 gtgcgcacca cgcgcccggt gtagatgcgc ccgataaccg gggtttcggt gatggcttca
     3361 acacgctcca ctgctcgtcg tgccgcctcg ccatcggctg tggcgataaa caccgagcca
     3421 tcttcgccga tgtcgatttt ggcgcctgtc tcttcctgga tggaccggat ggttttgcca
     3481 cccggtccga tgaccgctcc gatcttttcg accgggatct gcaccacggt gatgcgcggg
     3541 gcgtgcggtt tcagggtcgg atttggtgcg gggatgactt ccatcatttt attcaaaatg
     3601 tagaggcgcg cctggcgggc ctgctcgaga gcctgcctca tgatggtggt attaatgcct
     3661 ttgatcttga tgtccatctg cagggcggtg atgcccgcct gggtcccagc gaccttgaaa
     3721 tccatatctc cataatggtc ttctgcgccc aagatgtcgg taaggatggc gtagcgctcc
     3781 ccttccttga tcagtcccat ggcaactccc gcaaccggcg ccttgatggg cacgccagtg
     3841 tccatgagcg ccagggtcga gccacacacc gatgccatag acgaagagcc gtttgaagat
     3901 agcacttcag acaccaggcg caaggtgtaa gggaaatctg cttcaagcgg tatcacaggg
     3961 acgagggctc gttctgccag cgcgccatga ccaatatcac ggcgggatgc cccgcgcaaa
     4021 ggtctaacct cgccagtaga gaaaggcggg aagttgtaat gatgaatgta gcgtttagac
     4081 tcagacggcg acagactgtc gagttcttga gcttccctag gtgtgcccag ggtagccagg
     4141 gtgagcacct gcgtctcgcc acgtgtgaat aatccagacc catgcgcccg cgggctgacg
     4201 tccacctcac accagatatc gcggatgtcg ttcagaccac ggccatcagg acgctggttg
     4261 tgctccagga tggtctggcg cacgaccgtc ttgagtagct ggtcaaaggc ttcttttaca
     4321 agaggcgcct gtgactcatc ctccccagcc agatcctcta gcagggaggt tttgagcgca
     4381 tcgatggctt cattgcgttc ggatttaatg tagggttgtt gcaggatatg gttcagttgc
     4441 gctccagcgc gctcttgcac ggtcactgcc agatcctcgt tggccggggc ggagatgtat
     4501 tctctcttgg taacgccggc ttctgcaacg atgcgttcct gggcatcaat cagcggctgc
     4561 atggctttgt gtccgaactc cagggcttca accatgatct cttcggatac agccgaggca
     4621 ccacattcca ccatgagaat cgcatcgcgc gtgccagcca tgcgcaagtc cagatcggag
     4681 tattccaggt cagcgaaggt tgggttcaag atgaagtctc cctcaatcca gcctacgcgc
     4741 actgcgccga caggaccagc ccaggggaga ccagccagca gcaccgcagc cgaagccgcg
     4801 ttgacagcca ggatgtcgat aggattctcg ccatcggtgg agagtgagta caggatgacc
     4861 tggatgtcat tgcgcaggtt tttggggaac agggggcgca atggacggtc ggtaaggcgg
     4921 gcggtcagga tggcctcctc ggtaggcctg ccttcccggc gaaagaatga gccgggtatg
     4981 cgcccaccgg catacatgcg ctcttcgtaa tccactgtga ggggtacgaa gtcgacacca
     5041 gggcgaacat cagcactcat ggtggctgct gcgaggacca cagtatcgcc caggcgcaag
     5101 gtgaccgagc cgcctgcctg tccggcaagc tttccggtct caaacgtaaa tactttgctt
     5161 ccaagcgtgg cttggaattg cttaacatcg ggtttcattt tatctcctga attaccccac
     5221 ctggtcaggg actgagagct gccgacaaca cctgcacagg gttatcgcca tctttcaata
     5281 cctaaccggt gggcatgaaa aaaaatttgg aatcttttgg gttgagcgag agaagcccca
     5341 gaattcggtg tatagcgggt atacc
//
LOCUS       DBNHOH010000948         3491 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1471344, whole genome shotgun sequence.
ACCESSION   DBNHOH010000948 DBNHOH010000000
VERSION     DBNHOH010000948.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1471344"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1564
                     /locus_tag="AC16V7_17080"
     CDS             <1..1564
                     /locus_tag="AC16V7_17080"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6258891.1"
                     /translation="TQQTNQLGILKAIGGTSFTVLRVYLSGVVIYGLLSLGIALPLGS
                     LASFRISSWFLSLYNIEYTQFTSSPQATIFMVVAALVVPLAAALIPILQGAAITVRQA
                     IASYGLGGDFGSNWIDRLVEGIGRRFLISYQAMALANTFRRKGRLLLTELVLIIAGVM
                     FLMVMSLSSSIDATLDAEFARRDYDVVISFDDLQRVDRTVAMMEATPGVDHAGMWLAV
                     PATILHQGQKMLDAGLGSQLQGVPVEDPMYKPLIVEGRWLQPGDRRVIVMNKTTADDE
                     DIRVGDTVKLDLGVMGESDWQVVGLYRVFLMFGGGFNIDALYGPRDVVFAATKKTGRA
                     NLLLVRTQNHAPEAVELTASQLGDLFREHNIEISQTETMPSTRRTSDVSFSYVVIMLL
                     VLAVIVAMVGGIGLMGALWISVIERTKEIGVMRAIGALSRTIMGMFILEGIVQGLMSW
                     VIAVPLSLLVTPIMANALGMAMFNSRLDFQFNIQAVFIWLGTILVVALVAAIIPARNA
                     TRINVRQSLNYE"
     gene            2110..>3491
                     /locus_tag="AC16V7_17085"
     CDS             2110..>3491
                     /locus_tag="AC16V7_17085"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR6258892.1"
                     /translation="MAKPVILAIDDDPMVLSAVDRDLRKKYGQDYRIVPINMGSAAMQ
                     YLKQLQGRAEVVALFLVDQRMPQMSGVEFLEQARHVYPEAKKVLLTAYADTEAAINSI
                     NKVSLDYYLMKPWDPPEERLYPVLDELLGEWKAHVRMPYEGIRLAGTLWSSASHNTRD
                     FLTRNLIPYQWLDVEKDAKVRALVEEYNQGELRIPTLFFPDGSVMVEPNLRELAEKVG
                     LKTRASLPYYDLIIIGSGPAGLSAAVYASSEGLSTLAIERGTPGGQAGSSPKIDNYLG
                     FPNGISGNELARRAMTQARRLGAEILTAQEVTHISLQDPYRIVRLSDNTEISCRAVLL
                     ATGASFHMLRMPGAAALSGAGVYYGAAYTEARSYKDQDVFVVGGANSAAQGALYLSRF
                     ARKVNLIVRGELSAAQYLADELRQNEKIDIYLNTDLIEVHGEGKLEEIVVKNNRIDEL
                     QTLKGAAMFV"
BASE COUNT          780 a          798 c         1038 g          875 t
ORIGIN      
        1 cacccagcaa accaaccagc ttggcatcct caaggcgatt gggggcacca gctttacagt
       61 tttgcgtgtt tatctgagcg gggtggtgat ctatgggctg ctctcgttgg gcatcgccct
      121 gcccctgggg tcgctggcgt cctttagaat ctctagctgg ttcctgagct tgtataacat
      181 cgagtacact caatttacat cgtcgccgca agcgactatt ttcatggtgg tggccgcact
      241 ggtagtgcct ttggccgctg ccctgatccc tatcctgcag ggcgcggcca tcacggtcag
      301 gcaggctatc gccagctatg gcctgggtgg ggattttggc tcgaattgga ttgaccggct
      361 ggtggagggc attgggcggc gcttcttgat ctcgtatcaa gctatggctc tagccaatac
      421 attccggcga aaaggacgat tgctgctcac cgagttggta ctgatcatcg ccggggttat
      481 gtttttgatg gtgatgagtc tttcaagctc gatcgacgcc acgctggacg ctgaattcgc
      541 gcgccgcgat tatgatgtcg tcatctcctt tgatgatttg caacgcgttg atcgaacggt
      601 tgctatgatg gaggcaacac ctggggtaga tcatgccggt atgtggctgg cagtgcctgc
      661 caccatattg catcaagggc agaaaatgct ggatgccggc ctcggctcgc aattgcaagg
      721 ggtgcccgtc gaagatccca tgtacaagcc attgattgtg gaaggtcgct ggctgcaacc
      781 gggcgataga cgggtgatcg tgatgaataa gaccaccgcg gacgatgagg atatccgggt
      841 gggcgataca gtgaaactgg acctgggcgt catgggagaa agcgactggc aggtagtggg
      901 tttataccgg gtgtttctga tgttcggcgg gggcttcaat atcgatgcgc tctatgggcc
      961 gcgagatgtg gtctttgccg ctaccaaaaa aaccggcagg gctaatcttt tgttggtgcg
     1021 cacccaaaac cacgcacctg aggcagtaga gctgaccgcc agccagcttg gcgatctttt
     1081 cagggagcat aatatcgaga tttcacagac ggaaaccatg ccatcaacgc gcagaacgtc
     1141 tgatgtatcg ttttcgtatg tagtcatcat gctgctggtg ctggcggtca ttgtggcaat
     1201 ggtggggggc atcgggctga tgggggcgct atggatcagc gtgatcgagc gcaccaaaga
     1261 aatcggggtg atgcgagcca tcggggcgct ctcgcgaacg attatgggta tgttcatatt
     1321 ggaaggcata gtgcagggat tgatgagctg ggttattgcc gtgccgctct cgctgctggt
     1381 cacacctatc atggcaaacg ctctgggcat ggcgatgttt aattctcgtc tagattttca
     1441 atttaacatt caggcggtat tcatctggtt gggcacgatc ctggtggtgg cattggtagc
     1501 cgccattatc cctgcccgca atgcgacgcg catcaacgtg cggcaaagcc taaattatga
     1561 gtaggaaggg tatattagag aattattata cacatagtct tgtatacagt ccctgaggat
     1621 ggtatgatat tcgttgtgaa ttgtcgggtg gcaagcatat cctcacaata gggagctgtt
     1681 tgagcaagga tggggcgatt agcagacatt ctgaagatgc ttctggtgta tggtcgcagg
     1741 gtggctttaa ttatagggat ggttatgacc atcatcactt tctacgggtc gggtgcggtc
     1801 gatatcaccg ctcgttttta ttttcgttct ggaattcagt atttagtgta tctatccttc
     1861 atagtcggta tattgacatt catcaagctg atggaatttt ttggattgtg tgacgagttg
     1921 acgttatctt tccttgacga tgatgtgcgc tttttgagag cgattactgc taggcatcaa
     1981 cttggttgtg atatagtaat accgttcaac atacgcttat tcgcgccaat agtggtcgga
     2041 tagacaatcc gatttatttt aagtgcggct ggcgccgcgc acttgaatat aatcaaagaa
     2101 agaggcgaga tggcaaaacc agtaatcctt gcaattgatg atgatccgat ggtgctgagc
     2161 gccgtggacc gcgacctgcg aaagaaatat ggtcaggact atcgaatcgt cccaatcaat
     2221 atgggcagtg ccgcaatgca atatctgaag caacttcaag ggcgagccga ggtcgttgcc
     2281 ctgttcctgg tagatcaacg catgccgcag atgagtggcg tggaatttct tgagcaggcg
     2341 cgccatgtgt acccggaagc aaagaaagtg ttgttgactg cctatgcgga taccgaggcg
     2401 gcgattaact ctatcaataa agtcagcctg gattactacc tgatgaaacc atgggatcca
     2461 cccgaggagc gtctatatcc ggtgcttgac gaattgcttg gggagtggaa ggcgcatgtc
     2521 aggatgccat atgagggtat ccgcctggcg ggcactttgt ggtcatctgc ttcgcacaat
     2581 accagggact tcttgacacg caacttgatc ccataccagt ggttggacgt tgaaaaggat
     2641 gccaaggtcc gcgccttggt cgaagaatac aaccagggag aattgagaat acccaccctg
     2701 ttcttcccgg atggttcggt gatggttgag ccaaacctgc gtgaactggc tgaaaaagtt
     2761 ggcttgaaga cccgagcgtc attgccatac tacgatttga tcatcatagg cagcgggccg
     2821 gcagggttgt cggcggcggt atatgccagc tcggagggat tgagcacttt ggcaatagaa
     2881 agaggcactc cgggcggtca ggctgggagc agcccaaaga tcgacaatta ccttggtttc
     2941 ccgaatggta tctcgggcaa tgagctggcg cggcgcgcca tgacgcaagc ccgccgtttg
     3001 ggggcggaga tattgaccgc tcaggaggtt acccatatca gcctgcagga cccttatcgc
     3061 atcgtgcggc tatccgataa caccgaaatc tcatgccggg ctgtgttgct ggctacagga
     3121 gcttcttttc acatgttgag aatgccaggc gcagctgccc tgtctggggc aggggtctat
     3181 tatggtgctg cctataccga ggcacgctcg tataaggacc aggatgtgtt tgttgttggt
     3241 ggggcgaatt cggcggcaca aggggcattg tacctgagcc gttttgcgcg taaggtgaac
     3301 ttaattgtgc ggggtgagct cagcgctgcc cagtatctgg cggatgagct ccgccagaat
     3361 gaaaagatcg acatctatct caacacagac ctcatcgaag tgcacggaga gggaaaactg
     3421 gaagagatcg tggtgaagaa taaccgcatt gacgagcttc aaaccctcaa aggggcagcc
     3481 atgtttgtgt t
//
LOCUS       DBNHOH010000949         5603 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_958538, whole genome shotgun sequence.
ACCESSION   DBNHOH010000949 DBNHOH010000000
VERSION     DBNHOH010000949.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5603)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_958538"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /gene="pstC"
                     /locus_tag="AC16V7_17090"
     CDS             <1..822
                     /gene="pstC"
                     /locus_tag="AC16V7_17090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HIR6258893.1"
                     /translation="WPILAIISPWELLFGRIWQPLKGAFGFYPFILGTVWVTGIAMTL
                     AVPPALLCAIYLAEYARPATRVVAKPVLDLLASIPSVVYGVWGVVAIVPWVQGRVAPL
                     LNRWLGYLPLFRTTNPTGFGLLSGGIVLAVMVAPFIIAVTYEVVCAGPAGVREASLAI
                     GATQWQTIRHVLLPHTLPGILASIVLGASRALGETMAVLMVVGNVAQVPVSIFDAAYP
                     LPALIANNYGEMLSIPLYDAALLGAAFILLLIVVIFNLASRLVLRHLTGREVVKR"
     gene            819..1679
                     /gene="pstA"
                     /locus_tag="AC16V7_17095"
     CDS             819..1679
                     /gene="pstA"
                     /locus_tag="AC16V7_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR6258894.1"
                     /translation="MKISISRRRLEEGLVKTIMRASLLLVAGSLGLILATVIVKGLPS
                     LDLAMLTQTPKGGYYLGKEGGILNAIVGSLYLAGGATILALMLSLPIALYLQSYARRS
                     RWATLVRLGLDVLWGIPSIVYGAFGFSIMLALGMRASLLGGILTLALLELPIMTRAMD
                     EVIRMMPFDLEAASYAIGATRLETATRVVVRQMLPGILTALLLAFGRGIGDAASVLFT
                     AGYTDRIPMSLFRPVASLPLAVFFQLGTPFPEVQARAYSAALVLTVIVLGVSLCSRWL
                     AARLGRYVIR"
     gene            1695..2450
                     /locus_tag="AC16V7_17100"
     CDS             1695..2450
                     /locus_tag="AC16V7_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003501535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HIR6258895.1"
                     /translation="MDAHIQIRNLNVYYGKTHALKDITLNIPKRQITVIMGPSGCGKT
                     TLLRCLNRFLELTDHARLEGQILIDGENPYAAGVDVTEIRKRTGLLAQRPHPLPMSIY
                     DNVAYGLRVHSLCKRGCDLDDRVRHYLEVAGLWDEVKNRLHAPASGLSLGQQQRLCLA
                     RGLAVEPEILLGDEPTSALDPISALHIEARLLELKQSYTTVIVTHILRQARRLADYVI
                     FMYLGEVVEHGPAHQLFEHPEKPRTRAYLEGQF"
     gene            2603..3922
                     /locus_tag="AC16V7_17105"
     CDS             2603..3922
                     /locus_tag="AC16V7_17105"
                     /inference="COORDINATES: protein motif:HMM:NF051235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR6258896.1"
                     /translation="MTRRIWLLLCIVAGILVLWPWKATQAQGESPLVLVLTADGPLTP
                     AMLEYLERGIRTADDRGAELLVFQLNTPGGSIDLMQDMVEAIRSSPVPVAVYITPRGA
                     TAGSAGTVITLAGHVAAMAPETAIGAASPVGMEGQELGETIEAKAKNILKATVRSLAE
                     RRGPQAVDLAERTIEVADAASANEALEVGLVDFIADDLTGLLDQLDGFTVELITGERE
                     LNTANADIVPLSLSFIEQLLNMLTNPNIVFLLIAIGVQAVLIEISSPGGWVAGFIGVV
                     CLALATYGLGILPVNWFGIVFLILAFALFILDIKAPTHGALTAAGVGSLIVGALVLFN
                     SPSIPTFQRVSVPLVVGMSLITGATFFTILMIALRAQRSPIRIGQESLAGRRGVVRTR
                     LSPVGTVQVGGELWTAEPVEGEEALEPGTRIQVERVQGVRLYVRKIG"
     gene            complement(4006..>5603)
                     /locus_tag="AC16V7_17110"
     CDS             complement(4006..>5603)
                     /locus_tag="AC16V7_17110"
                     /inference="COORDINATES: protein motif:HMM:NF027484.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HIR6258897.1"
                     /translation="NEAIIGAPASTSGTLKGSGMGREMNRVQNACILLLLLLVILAAA
                     CAPVSLIPTAEPLESVYTAAAQTVAVELTLEAGQTAVARLTEMAQQQPTPTIWLPTLP
                     PPPPPTSTPPPPAPTSTVTPLPTQPTCDTASFVADITVPPNATFSPGERFVKIWRVQN
                     TGDCTWTSQYTLAFSGGEGMGSPLLVSLPATVNAGQSVDVPIELVAPSTPGFHQGYWK
                     LFDPNNAPIDIAPGPLGALWVQIQVVDTVSTSGEYDFAREYCAAQWRSNRDVLTCSGF
                     SDDPDGAVILLADPRLESRNEDELALWMIPGQGGGGWITGEYPPMRIYGGDRFVSEIG
                     CLRDNPDCELLFELGYRTQDGNVRTLDSWFESYDGDTREIDLDLEDLAGETVQFILSV
                     TNQGDQDDANGFWLVPHIHNLSQRSDLVISWRQEGGTNEVCYDVKLYLTGRHDGEARA
                     RSCGGGIRESGTLRLNDAQVDLVLDWLDTFRPYEFEVETPTNNEDLVEKIIFDGEGDI
                     EVLYEDIRSMQNFMRSLYNAIIF"
BASE COUNT          979 a         1633 c         1755 g         1236 t
ORIGIN      
        1 tggccgatct tagccatcat ctccccttgg gaattgctgt tcgggcgcat ctggcagcct
       61 ctcaagggag catttggctt ttatccattc atccttggga cggtttgggt aaccgggatc
      121 gcgatgacac tggctgtgcc accggccttg ctgtgcgcca tctacctggc agaatatgcc
      181 cgcccggcta cacgggtggt cgctaagcct gtgcttgact tgctggcctc aatcccttcg
      241 gtggtatatg gcgtgtgggg cgtggtagcc atcgtcccct gggtgcaagg gagagttgcc
      301 ccgctgctga atcgctggct gggttacttg ccactttttc gtactactaa cccgacgggc
      361 ttcggcttgc tctcgggggg tattgtgctg gcggtcatgg tagcgccgtt tatcatcgcg
      421 gtcacctacg aggtcgtttg cgcgggaccg gctggggttc gcgaggcctc gctggctatc
      481 ggcgctacac agtggcagac catccgccac gtcctgctgc cccacacctt acctggcatc
      541 ctggccagca tagtactggg agcctcccgc gccctgggcg agactatggc agtgctcatg
      601 gtggtgggca atgtggccca ggtgcctgta tccatcttcg atgctgctta cccgctaccg
      661 gctttgattg cgaataacta cggtgagatg ctttccatcc cgctttatga tgcggccttg
      721 cttggggcag cctttatctt gctgctgatt gtggtgattt tcaacctggc atccaggctg
      781 gtgctgcgcc acctcaccgg aagggaggtt gtgaagcgat gaaaatttct atttcccgac
      841 gccgcctgga agaagggctt gtcaaaacca ttatgcgggc ttccttgctg ctggttgcag
      901 gcagcctggg attgatcctg gcgaccgtga tcgtcaaggg gctgcctagc ctggatctcg
      961 ctatgctcac ccagacacct aaaggcggct actacctggg caaggagggc ggcattttga
     1021 acgccatcgt tggatcgctg tatctggctg gtggagcaac tatcctggcc ctgatgctca
     1081 gcctgcccat cgccctatac ctgcagtcgt atgcccgccg ctcgcgctgg gctaccctgg
     1141 tgcgcctggg tctggatgtc ctgtggggca tcccatccat cgtatatgga gcctttggat
     1201 tcagcatcat gctggctctc gggatgcggg cctcgctgct gggaggcatc ttgacgctgg
     1261 cgctgctgga gctgcccatc atgacccgtg ccatggacga ggtgatacgc atgatgcctt
     1321 ttgacctgga ggctgcttcc tatgctattg gagccacccg tttggagaca gctacgcgcg
     1381 tggtggtgcg gcagatgctg ccgggcatcc tcaccgcgct gctgcttgcc tttgggcggg
     1441 ggattggcga tgcggcttct gtgctcttca ccgcaggtta tacggatcgt atccccatgt
     1501 ctttattccg ccctgtggca tccttgccgc tggcggtatt cttccagcta ggcaccccct
     1561 tcccagaagt gcaggcgcgg gcatattctg ccgcgctggt gctgacggta atcgtgctgg
     1621 gcgtcagcct gtgctcgcgc tggctggctg ccaggctggg caggtacgtc atccgctgat
     1681 ctcggaggtt gccaatggac gcgcatatcc aaattcgcaa tttgaatgta tattatggaa
     1741 agacgcacgc tctgaaggat atcaccctga atatcccgaa acggcagatc acggtgatca
     1801 tgggcccttc gggttgcgga aaaaccaccc tgctccggtg cctcaaccgc ttcctggagc
     1861 ttaccgatca cgcccgcctg gaggggcaga tcctcatcga tggggagaat ccctatgccg
     1921 caggggtgga cgtgaccgaa atccgcaagc gtaccggctt gctggctcaa cgcccgcatc
     1981 cgctgccgat gtctatttac gataacgtcg cctacggtct gcgagtgcat tccttgtgca
     2041 agaggggctg cgacctggac gaccgcgtgc gccactactt ggaagtggca ggattgtggg
     2101 acgaggtgaa aaaccgcctg catgccccgg catctggttt gagccttggg cagcagcagc
     2161 gattatgcct ggcgcgcggc ctggcggtcg agcctgagat cttgttgggg gatgagccga
     2221 cctcggcgct ggatccgatc tcggccttac acatcgaagc acgcttgctg gagctgaagc
     2281 agtcttatac caccgtgatc gtgacgcata tcctgcgcca ggctcgccgc ctcgccgatt
     2341 acgtgatctt catgtacctt ggcgaagtgg tggaacacgg cccggctcac caacttttcg
     2401 agcaccctga gaagccgcgc acgcgagcct acctggaagg gcaattctag ggcaatcgca
     2461 ttaggacgat ttcccacacc aatcactctc tttacgggat tgcgcttgcc ctggggcaat
     2521 tctgagccgg gtatccaggc ctgccagatt tgctataatt gagttatgga ttgagcggtt
     2581 ccactcaatc gtggagttga ttatgaccag gcgaatatgg ctactgcttt gcatcgttgc
     2641 gggaatactc gtattatggc cctggaaggc aacccaggct cagggcgaat cgcctctggt
     2701 tttggtgctg accgccgatg gcccgctcac accggctatg ctggaatacc tggagcgcgg
     2761 cattcgcacg gctgatgacc gtggggctga gctgctcgtt ttccagctga acaccccggg
     2821 tggcagcatt gacctgatgc aggatatggt cgaagccatc cgctccagcc ctgtgcccgt
     2881 ggcagtgtac atcacaccgc gcggcgccac ggcgggcagc gctggcacag tgatcaccct
     2941 ggcggggcat gtggcagcca tggcgcccga aacggctatc ggagcagcca gcccggttgg
     3001 catggaaggg caggagctgg gcgagaccat cgaagccaaa gccaagaaca tcctcaaagc
     3061 cacggtgcgc tccctggctg agcggcgcgg cccgcaggcg gttgatttgg cggagcggac
     3121 gattgaggtg gcggatgcgg cttctgccaa tgaagccctg gaggttggtc tggtggattt
     3181 catcgccgat gatttgaccg gcctgctgga tcagctggat gggttcacgg tggagttgat
     3241 caccggcgag cgggagctga acaccgccaa tgctgacatc gtgcccctct cgctctcctt
     3301 tatcgagcag ctgctcaaca tgctcaccaa cccgaatatc gtcttcctgc tgatcgccat
     3361 cggcgtgcag gcagtcttga ttgaaatctc cagcccgggg ggttgggtgg cgggcttcat
     3421 cggcgtggtg tgcctggcgc tggcgaccta cggactgggc atattgcccg tcaactggtt
     3481 tgggatcgtg tttctcatcc tggcttttgc gctcttcatc ctggatatta aagcgcccac
     3541 acacggggca ttgaccgccg ccggcgtggg ctcgctgata gtcggcgcgc tggtgttgtt
     3601 caactcgccc agcatcccca ccttccaaag agtgtcggtg ccgctggtgg tgggcatgtc
     3661 gctcattacc ggggcaacct tcttcacgat actgatgatc gccctgcgcg cccagcgcag
     3721 ccctatccgc atcgggcagg aaagcctggc tgggcgcagg ggcgtggtgc gcacccgcct
     3781 gtccccggtg ggtaccgtgc aagtgggcgg cgagctgtgg acggcggagc ccgtcgaggg
     3841 ggaagaagcc ctggaacctg gaacgcgcat ccaggtcgag cgcgtgcagg gagtgcggct
     3901 atacgtgcgc aagatcggct agcccgtttt ttcgttagtc atcctctacc ccagtggtcc
     3961 aggggtggga gattgtcaaa agcattctaa tctacggtac ggcggttaga agatgatggc
     4021 gttgtacagg ctccgcatga aattctgcat gctgcggata tcttcgtaga gcacttcgat
     4081 atccccctcg ccatcgaaga tgatcttctc gaccaaatct tcgttgttgg taggcgtctc
     4141 gacctcaaac tcgtagggcc tgaatgtgtc caaccagtct aagaccaggt caacctgggc
     4201 gtcattcaag cgcagggtgc ccgattctcg tatgccgccc ccacagctgc gggcgcgcgc
     4261 ctcgccgtca tggcgcccgg tcaggtacag cttgacatcg taacaaacct cattggtgcc
     4321 cccctcctgg cgccagctga tgaccaggtc tgaacgctgg gagagattat ggatgtgcgg
     4381 cacaagccag aatccgttgg cgtcatcctg gtctccctgg ttggttacgc tgaggataaa
     4441 ttgcaccgtc tcgcctgcca ggtcttccag gtccaggtca atctcgcggg tgtcgccatc
     4501 gtaggattca aaccaggagt ccagtgtcct gacattgcca tcttgggtgc ggtagcccag
     4561 ctcgaagagc agctcgcaat ccgggttgtc cctgagacag ccgatctccg agacgaacct
     4621 gtcgccgcca tagatgcgca tcggcgggta ctctccggtt atccagccac ccccaccctg
     4681 acctgggatc atccacaaag ccagctcgtc ttcgttgcgc gattccaggc gcgggtcggc
     4741 caggagaatc acggctccat cggggtcatc gctgaagccc gaacaggtga gcacatcccg
     4801 gttgctgcgc cattgggcgg cgcagtactc cctggcgaag tcatattcgc cgctggttga
     4861 aaccgtatcc accacctgga tctgcaccca caaagcacct aacgggccag gggcaatatc
     4921 gatcggggcg ttgtttgggt cgaacagctt ccagtagcct tgatggaaac ccggcgtgga
     4981 tggggcaacc agctcaatgg gcacatccac cgattgaccg gcgttgaccg tggcaggcaa
     5041 actgaccagt aacgggctgc ccatgccttc gccgccagaa aaggccagcg tgtattgcga
     5101 cgtccaggtg cagtcgcctg tattctggac gcgccagatc ttgacgaagc gctctcccgg
     5161 cgaaaaggta gcgttcggcg gaaccgtgat atcggctaca aagctggcgg tatcacaggt
     5221 gggctgggtg ggtagggggg ttacggtaga ggtcggggca ggcggtggcg gggtgctggt
     5281 gggcggtggc gggggtggga gcgtgggcag ccagatggtc ggcgtgggtt gctgctgggc
     5341 catctcggtc aggcgggcga cggctgtctg gccggcctcc agggtcagct ccacggctac
     5401 ggtctgggcg gcggcggtat agacggattc caggggctcg gcggtgggga tcaggctcac
     5461 tggcgcgcaa gcagcggcaa ggattaccaa gagcaggagc aataggatgc atgcattttg
     5521 taccctgttc atctctcgtc ccataccgga tcccttgagc gtgcctgagg tactggctgg
     5581 cgcccctatt atagcctcgt ttt
//
LOCUS       DBNHOH010000950         3355 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_701928, whole genome shotgun sequence.
ACCESSION   DBNHOH010000950 DBNHOH010000000
VERSION     DBNHOH010000950.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_701928"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="AC16V7_17115"
     CDS             <1..351
                     /locus_tag="AC16V7_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HIR6258898.1"
                     /translation="APNGDGAVGGNPAVGPFLAQKKGVQLTVPPLPSEAGWKDTVIMY
                     PGEVTRIMVRWAPTDVTVGDTANEHFPFDPNAGHGFVWHCHIIDHEDNEMMRPNLVDP
                     KTGVTRTYVKGTDY"
     gene            521..1861
                     /locus_tag="AC16V7_17120"
     CDS             521..1861
                     /locus_tag="AC16V7_17120"
                     /inference="COORDINATES: protein motif:HMM:NF024935.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR6258899.1"
                     /translation="MNKKETTQENLNKIIEFRQAIYAHGMSKRRDALFEAWDAMVLHG
                     MKTSFPWLSLSGVFRRKWHSLYKALEQGEIDMNWLASYLAQQVPQAALCHFSLDGTGW
                     PRTRARTMDDRQYLYLPTQAVNGGSICVGYPYSLLDWVPEPQSSWSLSVSVRRISSNE
                     SAQSVGVEQVMALNEARKDYQEVLDIVAGDGKYGNANFLRPLRGQRCGIVVRLRKDRI
                     LYRKPDEPKTRKRGRPRVHGRRFAFKEPDTWDDPDEVIELEDVCWGQVRLERWRDLHG
                     RVDADVPFDVIRASVHVERDKPPLPIWLAWQAPACIPQDILVDAAIIWRAYVHRWPVE
                     PNIRFRKQHLGWCKPQFQDKETGDRWSWLVALAVWLLYLVRPIVQDQPFPWQKPQTHL
                     TPQRVQQGLPLIFAQFGSPARKPKVRGIPPGWLKGRCRKPKPRFAVVKKLPSPA"
     gene            1979..2230
                     /locus_tag="AC16V7_17125"
     CDS             1979..2230
                     /locus_tag="AC16V7_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycoredoxin"
                     /protein_id="HIR6258900.1"
                     /translation="MTESKITIYGTTWCGDCRRTRRFLDYHKVSYHWIDIDQDKDGEE
                     FVFTINQGMRSVPTIVFPDGSILVEPSNIKVAEKLGLPT"
     gene            2244..3236
                     /locus_tag="AC16V7_17130"
     CDS             2244..3236
                     /locus_tag="AC16V7_17130"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR6258901.1"
                     /translation="MNPSPKKALVLGATGFIGGHIALAALERGWQVRGFRRDPLATGN
                     LANTSVEWVNGNLDEPDSLRQAMQGVDIVFHAAGFYPSRKDHRSLSTQVAYALAQTNH
                     VLSATREANIERFIYTSSLSTITRSRSANNNLATERDSYQPGVMPKSTYYEAKYAMEQ
                     AVLQASLEGLPAVVLNPTAVFGPGDVHLTLGRLLVAIGRGWAIAWLPVTINVVDVRDV
                     AQAHIQAAMHGKVGQRYIIGGHNLFLKEALDLVANIANVHPPRFEIPLWLIDTIIWLD
                     DVIPMLNLSGNHLRAIRFWHGYDISKAQSELALSPRPFEETVRDALDWLQRHKM"
BASE COUNT          852 a          870 c          881 g          752 t
ORIGIN      
        1 gctcccaacg gcgacggcgc ggtgggcggc aacccggcgg tcgggccatt cctagctcag
       61 aagaagggtg tgcaactcac cgtcccgccc cttccgagcg aggcgggttg gaaggacacc
      121 gtgattatgt acccgggcga ggtgacccgc atcatggtgc gctgggcgcc gacggacgta
      181 actgtagggg ataccgccaa tgaacacttc cccttcgacc ccaatgccgg gcacggcttc
      241 gtctggcact gccacatcat cgaccacgaa gacaacgaga tgatgcgccc gaacctggtg
      301 gacccgaaga caggcgtgac gagaacctat gtgaagggca ccgactacta aacccggcac
      361 atcgcccacc ccgagtttcg tctctcgggc tgcgcggtag ccacgcacac ccctcccgca
      421 ggtgatagac ctgcgggagg ggttctgctg gaaatcgagt ttagacaaaa agtagggcag
      481 aacccttgca ggggactgcc cttgggtaga aacttgatgt gtgaacaaaa aagaaactac
      541 ccaagagaac cttaacaaga taatcgagtt tcgtcaagcg atttatgcac acggcatgag
      601 caagcgtcga gatgccctgt tcgaggcctg ggatgcaatg gtgctacatg ggatgaaaac
      661 atcctttcca tggctaagcc tgagtggcgt attccggcgc aagtggcaca gtctgtacaa
      721 agcgctggaa cagggtgaaa tcgatatgaa ttggctggca agctatctgg cgcagcaagt
      781 gccacaagct gcgctctgcc atttctcgct agacggcaca ggttggccac gcacacgagc
      841 acgcacgatg gatgatcgcc aatacctgta cttgccgacc caagcggtca atggtggatc
      901 aatctgtgtg ggctacccct acagtctgtt ggattgggtg ccggagccac agtctagttg
      961 gtcactgtcg gtcagtgtac gccgtatttc cagtaacgaa agcgctcaat ccgtaggtgt
     1021 ggaacaagtc atggcactca atgaagccag aaaagactat caagaggtgc tggacatcgt
     1081 ggcgggtgat ggcaaatatg gcaacgccaa tttcctgcgt ccactcaggg gccaacgctg
     1141 cggcattgtg gtgcgcttgc gcaaggatcg gatcctgtat cgcaagcccg atgagcccaa
     1201 aaccaggaaa cggggacgac ccagagttca tggtcgacgc ttcgctttca aagagccaga
     1261 cacctgggat gacccagatg aagtgatcga actggaagat gtgtgctggg gacaggtgcg
     1321 attggagcgt tggcgtgatc tgcatggcag ggtggatgcc gacgtgcctt tcgatgtaat
     1381 ccgagccagt gtgcatgtgg aacgagacaa accgccccta cccatctggc tggcatggca
     1441 agcacctgcc tgtatcccac aagacatcct ggtggacgca gctatcatct ggcgagctta
     1501 tgtccatcgc tggcctgttg aacccaatat tcgctttcgc aaacaacact tgggatggtg
     1561 caagcctcaa ttccaggaca aggaaaccgg cgatcgttgg agttggctgg tcgctttagc
     1621 agtctggctg ctttatctag tgcgtccgat cgttcaagat cagcccttcc cctggcagaa
     1681 accacagacc cacttgacgc ctcaacgtgt tcagcagggt ctgccgctga tttttgcaca
     1741 gttcggctca ccagctcgca agcctaaagt gcgaggtatt cccccagggt ggctcaaagg
     1801 ccgatgcaga aagcccaaac cccgttttgc ggtggtgaaa aagctgccta gcccggctta
     1861 gtcccctgaa actcgattgt taatgtgctg tttttcaatc ccgatccaag atcgggaata
     1921 catcatttgt ctaagtacga tttataataa tgcaaccaaa aaacaaagag accgtttcat
     1981 gaccgaatca aaaatcacca tttacggtac cacctggtgc ggcgattgtc gacgcacaag
     2041 gcgattcctc gattaccaca aggtatcata ccattggatc gatatcgatc aagataaaga
     2101 tggtgaggaa tttgtgttta ccatcaatca aggcatgcgt agtgtaccaa cgattgtctt
     2161 ccctgatgga tcaattttag tcgaaccttc aaatatcaag gtagcggaaa aacttgggct
     2221 gccaacatga tcaataacca gccatgaacc cttctcccaa aaaagccctc gttttaggcg
     2281 cgaccggatt tattggaggc catatcgccc ttgccgctct agagcgtgga tggcaggtac
     2341 gtgggttcag acgcgatcca ttggctacag gcaacctggc taatacaagc gtggaatggg
     2401 taaatggtaa tttggacgag cccgattcac ttcgacaggc catgcaaggg gtagacatag
     2461 tattccacgc cgctggattt tatccatccc gaaaagatca tcggtcatta tcgacacagg
     2521 tagcgtacgc gctcgcgcaa accaatcacg tgctttctgc tactcgggaa gctaacattg
     2581 agcgattcat ctatacttct tctcttagta caatcacccg atcacgatct gctaataaca
     2641 accttgcaac cgaaagagat agttatcagc caggagtaat gccaaaaagc acctattacg
     2701 aggcgaaata cgcgatggaa caagctgtat tgcaggcttc attggaaggt ttacctgccg
     2761 ttgttctaaa tcctacggct gtatttgggc ctggagacgt ccacttaaca ctaggacgct
     2821 tgctggttgc tatagggcgc ggctgggcga ttgcttggtt gccggtcacg atcaatgttg
     2881 tggatgtccg agatgtagca caggcgcaca ttcaagctgc catgcatgga aaagttggtc
     2941 agcggtacat tatcggaggt cataacctgt tcctaaaaga agcactagac cttgtggcaa
     3001 atatcgccaa tgtccatcct cctcgctttg agatacccct ctggcttatc gataccataa
     3061 tctggctgga tgacgtaatc cctatgttaa acctgagcgg caaccattta cgagcaatcc
     3121 gtttctggca tggttacgat atttctaaag cacaaagtga gctcgctctt tcgcctcgtc
     3181 catttgaaga gacagtcagg gatgccctgg actggctcca gcggcacaaa atgtgaagcg
     3241 atccgctata atgcactaat attagccata gtcaaagcgg ttaaagtaat tcctcttcac
     3301 aatcccaggt agttttatct ctcaatagaa tagaagttac gcagttggat acgtg
//
LOCUS       DBNHOH010000951         4080 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_637772, whole genome shotgun sequence.
ACCESSION   DBNHOH010000951 DBNHOH010000000
VERSION     DBNHOH010000951.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_637772"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..2009
                     /gene="dnaK"
                     /locus_tag="AC16V7_17135"
     CDS             105..2009
                     /gene="dnaK"
                     /locus_tag="AC16V7_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR6258902.1"
                     /translation="MSKIIGIDLGTTNSVVAVMEGGEPFVISTAEGSRLMPSVVAFNK
                     NGERLVGQVAKRQAVVNPENTIYSIKRFMGRRYDEVETERKMVPYQVAQGPTGDARVH
                     IPQTKREYSPQEISAMILGKLKADAEAYLGESITKAVITVPAYFNDSQRQATKDAGKI
                     AGLEVLRIINEPTAAALAYGLDKKKNETILVFDLGGGTFDVSVLDVGEGVIEVRATNG
                     DTHLGGDDWDERIVNWIADEFKKEQGIDLRQDRQALQRLREAAEKAKIELSSVHETEI
                     NLPYVTADSGGPKHLQIKLTRAKFEQLTEELVNRLRGPFKAALNDANLSASEVNEVVL
                     VGGATRMPMVQELVRSLTNKEPHKGVNPDEVVAVGASIQAGVLGGEVKDVLLLDVTPL
                     SLGLETLGSVMTALIERNTTIPVKKSEVFSTAEDNQTAVDIHVLQGERPMAGDNMSLG
                     RFRLEGIPPAPRGIPQVEVTFDIDANGILNVTARDKATGKEQKVTITASTNLSKSDID
                     RMVNEASAHESEDRKRRELVEARNMGDSLTYQTEKTLRELGDKVPADERKNIDEKIKA
                     VREAIQGDDINRIKQLQEDLQNAFHALSQQLYAQQAQAGDTSPPDNGHDRGPSSDGEG
                     EVIEGEYREA"
     gene            2242..3753
                     /locus_tag="AC16V7_17140"
     CDS             2242..3753
                     /locus_tag="AC16V7_17140"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR6258903.1"
                     /translation="MQSTVPGVNTSWEAQRTVKISQASRNIPRHWQWRLLIMCLIIGD
                     ALMIGIGFRVAYYLLFETVISRSFLATPDGFYLYREFVFTTVPIWLAIFATNRLYSRQ
                     NLLGASKEYSLVFKATSTGMLLVFAVGFLLPGFNLHRGWLLLSWLLTFLFTTTGRLLL
                     YRAVVHLREHGYYLSPAVIVGANNEGLSLAKQLIRWRSSGIHILGFLDKKLPAGKKVI
                     NHLYCLGSVDELEAIVERYGVEEIILATSAFSSRDKVLEIFRRYSNTNHVNVRISSGL
                     YEIITTGLTVKDIAFVPLVRINPIRLTKVDRALKLVFDFSLALLGTIFIFPFILLIAI
                     AIKLDSPGPIFSRQRIIGLNGKQFEAFKFRTEHSEEAVNLKAYPRLPAKPTSYQYYRN
                     NPRLTRVGKWLLRTNLDELPKIFNVLKADISLIGPRPITPVEMDKYARWDMNLLSVRP
                     GMTGLWQSFGRSDLSTSERIRLDMYYICNWSIWLDLQILFQSLLLAINGKGYY"
     gene            complement(3879..>4080)
                     /locus_tag="AC16V7_17145"
     CDS             complement(3879..>4080)
                     /locus_tag="AC16V7_17145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258904.1"
                     /translation="QILSRYYLRVTTIESDVLPALIQPFTVEDLGGWTQAHNTLIEDL
                     WMTEIKPYLALESANTLLGTAK"
BASE COUNT         1059 a         1016 c         1054 g          951 t
ORIGIN      
        1 ttaacggatt tcttgcacaa gtgcgttatc cttacctaca caagtgcgtt atccttacct
       61 atgaatataa actgacgaaa attaatcaga tagaggagag agttatgtcg aagattatcg
      121 gtattgatct gggaacaacc aacagcgtgg tagccgtgat ggaaggcggg gagccatttg
      181 ttatatctac cgctgagggc tcccggctca tgccttcggt tgtggctttc aacaagaacg
      241 gggagcgcct ggttggacag gttgccaagc gccaggctgt ggtaaaccca gaaaatacaa
      301 tatattctat taagcgcttt atggggcggc gatatgatga agttgaaaca gaacgaaaaa
      361 tggtgccgta tcaggtcgca caaggtccca caggagatgc tcgggtgcat atcccgcaga
      421 ccaaacgcga gtatagtccc caagagatct ccgcgatgat cctgggaaag ctcaaagcag
      481 acgcagaggc ttacctgggc gagtcaatta cgaaggcggt catcaccgtg cctgcatatt
      541 tcaacgacag ccagcgccag gctaccaaag atgctggcaa aatcgctggg ctggaagttc
      601 tacgcatcat caacgagccc accgctgcag ccctggctta tgggctggac aaaaagaaaa
      661 acgagacgat tctcgtcttt gacctgggtg gcggcacctt tgatgtcagc gtcctggacg
      721 tgggtgaggg cgtgatcgag gtccgtgcca ccaatggcga tacacacctg ggtggcgacg
      781 actgggatga gcgcattgtc aattggatag cggatgaatt caaaaaagaa cagggtattg
      841 acctgcgaca ggaccgccaa gccttacagc gcctgcgcga ggctgctgaa aaagccaaga
      901 ttgagctctc tagcgtgcac gagacggaga tcaacttacc ctatgtcaca gccgactcgg
      961 gcgggccgaa gcacttgcag attaagctca cccgcgccaa atttgaacag ctgacagaag
     1021 aattggtcaa tcgcctgcgc ggtcccttca aggcagcttt gaatgatgcc aacctgagtg
     1081 ccagcgaggt taacgaagtc gtgctggtag gtggtgctac gcgcatgcca atggttcagg
     1141 aattggtgcg atcgctcacc aacaaagaac cacacaaagg tgtcaacccc gacgaggtgg
     1201 tcgcagtcgg tgcttctatc caggccggcg tgctgggcgg cgaggtgaaa gacgtactgc
     1261 tgctggatgt cactcccctc tctttgggat tggaaaccct gggaagcgta atgaccgcgc
     1321 ttatcgagcg caataccacc atcccggtca agaaaagcga ggttttctct actgcagagg
     1381 ataaccagac cgccgtagat attcacgtgc tgcaaggcga gcggcctatg gctggtgata
     1441 atatgagcct tgggcgtttt cgcttggagg gcatcccgcc cgcgccgcgc ggcattcccc
     1501 aagtcgaggt caccttcgat attgatgcca atggcatcct caacgttacc gcccgcgata
     1561 aagctactgg taaagagcaa aaggttacca tcaccgcttc gacaaatcta tctaagagcg
     1621 atatcgaccg catggtgaac gaggcgagcg ctcatgaaag tgaagaccgc aagcgccgcg
     1681 agctggtaga agctcgtaac atgggtgata gcctaacata ccagacagag aaaacgctgc
     1741 gcgagttggg cgataaggtc ccagcggacg agcgcaagaa tatagacgag aaaatcaaag
     1801 cggtgcgtga ggccatccaa ggtgacgata tcaaccgcat taaacagttg caggaagatt
     1861 tgcaaaacgc attccacgcc ctgagccagc agttgtacgc ccaacaggcg caggcaggcg
     1921 acacctcgcc gcccgataac gggcatgacc gaggtcccag ctcagatggg gaaggcgaag
     1981 tgatcgaggg cgaataccga gaagcttaat cgataacaag tggttgtaaa agattaagca
     2041 gggatttgtg ggcagggttg cacccacaaa tcccttttct atttaattta aaagaaggta
     2101 acatttcgca taatatttcg ttaaccatat ttagagagtc tccgcccgga aaagacacct
     2161 cctctcagga ggaattcttg ggggaagccg caaggaaaaa ccaatctctc attgtccgca
     2221 tcgattttgg aggagagggg tatgcagtca actgtgccag gtgtgaatac atcctgggaa
     2281 gcccaacgta ctgttaaaat tagccaggca tccagaaata tcccgcgcca ctggcagtgg
     2341 cgtctgttaa tcatgtgcct aatcataggc gatgcgctca tgattgggat tggttttcgc
     2401 gtggcttact acctgctctt tgaaacagtt atctcgcgat cgttcctggc aacgccagac
     2461 ggtttttatt tatatcggga atttgtgttt accaccgtcc ccatctggtt ggcgatattt
     2521 gccacaaata gactttatag ccggcagaac cttttgggtg cttccaaaga gtattcctta
     2581 gttttcaaag ctacaagcac tgggatgctg ctggtattcg ctgtgggttt ccttttacct
     2641 gggttcaacc tccatcgcgg ctggctgctg ctgtcctggt tacttacatt tctattcacc
     2701 acaacaggtc gcttgctcct ttatcgcgcc gttgttcatc tgcgcgaaca cggatactat
     2761 ctctccccgg cagtgatcgt aggagcgaac aatgaggggc tctccctggc caaacagctc
     2821 atccgctggc gctcgtcggg aatccatata ttgggttttc tggacaaaaa attgccagcc
     2881 ggtaaaaaag tgataaacca cctctactgt ctgggatcag tggatgagct cgaagcaatt
     2941 gtcgaacgat atggtgtaga agagattatc ctagcgacca gcgccttctc atctcgtgat
     3001 aaggtgctgg agatcttcag gcgttatagc aacaccaacc atgtgaatgt gcgcatttcc
     3061 tcaggcctgt atgaaattat taccaccggg ctaaccgtca aagatattgc ctttgtgccg
     3121 ctggttagga tcaatcccat acggctgacc aaggtcgata gagccctcaa gctggttttc
     3181 gatttcagcc tggccctcct tggcacgatt ttcatttttc catttatttt gctaattgcc
     3241 atcgccataa agctagactc accaggaccg atattcagtc gccagcgcat cattggcttg
     3301 aacggcaaac agtttgaggc atttaaattc cgcacagaac atagtgagga ggctgtaaac
     3361 ctgaaggcgt acccacgcct tcctgcgaag ccaaccagtt atcaatatta ccgaaacaac
     3421 ccgcgcctga cacgggtcgg caaatggctg ctcaggacca acctggatga gttgcctaaa
     3481 attttcaacg tgttgaaggc tgatatctcg ttgataggcc cgcgcccaat aaccccggta
     3541 gagatggaca agtacgcccg ctgggatatg aatttgctct ccgtccgtcc gggaatgacc
     3601 ggtttatggc agagctttgg ccgctcagac ctgtcaacca gcgagcgcat ccgtttggac
     3661 atgtactata tttgcaattg gagtatctgg ttagacctgc agatactgtt ccagagcctg
     3721 ctgttagcaa taaacggaaa agggtattat tgacccccag ataacttagc tataaacgtc
     3781 ccgtagtttt atgctcaagg cgatcatttc gtacgtgaaa tatcgcatgt tcccctttaa
     3841 aatccaattg agcaaggacg agaatgataa tctcatggct attttgctgt cccaagcaaa
     3901 gtattggcag attccaatgc aaggtacggc ttaatttctg tcatccataa atcctctatc
     3961 aacgtgttat gggcctgtgt ccaaccgccg agatcttcca ccgtaaaagg ttgaatcaac
     4021 gcaggcaaaa catcgctttc aatggttgta acccgcaggt aataccggct taatatttgc
//
LOCUS       DBNHOH010000952         5212 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_509644, whole genome shotgun sequence.
ACCESSION   DBNHOH010000952 DBNHOH010000000
VERSION     DBNHOH010000952.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_509644"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="AC16V7_17150"
     CDS             <1..382
                     /locus_tag="AC16V7_17150"
                     /inference="COORDINATES: protein motif:HMM:NF024874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR6258905.1"
                     /translation="TTDDPRNSLYVFDYNGINVAHLGDLRRVPSQTEIEALGTVDVAL
                     VPVGGGGGLNAAKAAEVVSLLEPGIVIPMHFSTPASLLKLAPLSKFLKEMGLGKTEQQ
                     DSLKVTRTSVPEETRVVVLNYVRS"
     gene            397..699
                     /locus_tag="AC16V7_17155"
     CDS             397..699
                     /locus_tag="AC16V7_17155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258906.1"
                     /translation="MSVKLLMTWDILPGREQEYFEFVVRDWIPGIQRLGLEPSDAWFT
                     MYGDQPQILAAAQSSSLSSLQHILNSPDWKTLTQQLLDYVEDFNYKVVSARTGFQL"
     gene            726..1835
                     /gene="mutY"
                     /locus_tag="AC16V7_17160"
     CDS             726..1835
                     /gene="mutY"
                     /locus_tag="AC16V7_17160"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011243168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HIR6258907.1"
                     /translation="MAFGQDQQSCVMAFLFTENLLDWYANNARDLPWRGHCDPYAIWV
                     SEIMLQQTRVETVIPYFERWMQRFPTIAALAAADQQDVLAVWEGLGYYSRARNLHKAA
                     GVVVDQHGGVLPSDVTALRRLPGIGRYSAGAIASIAFGQDEPALDGNIRRVLARYFDV
                     SEPARATSGERRLWALAAMHLLTGRAADYNQAIMDLGATICTARAPDCPRCPLREGCR
                     ARLLGVQEERPVLAKKTSQPHHIVTAAVIRQDGRILIAQRPARGLLGGMWEFPGGKLQ
                     PGEDLPTCLQREIREELGAQIMVGGRLGVYQHAYTHFRVTLHAFECSLKGASLPQPRE
                     HAGLRWVIPAQLVDYPMGKIDRRIATHIIESEAEC"
     gene            1829..2917
                     /locus_tag="AC16V7_17165"
     CDS             1829..2917
                     /locus_tag="AC16V7_17165"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HIR6258908.1"
                     /translation="MLIVDAHQDLAWNTLTFGRDYTLPISETRLREQGSVAVEHNGDT
                     LLGWPEYQRGQVGVIFSSLFASPARLRSGDWDSQTYRDFMEANARYRAQLDVYHRLVD
                     QHSDKFRLVKGNDDLQEVVDHWKQNTAQEHPIGLVILMEGAEGVQEPSELGEWWDGGV
                     RLIGPAWAGNRYCGGTREPGPLTSEGYALLDGMADLGFILDISHMDEQAALQALDHYP
                     STIVATHANAITLLKGLDTNRHLSDRVIRMLIERGGVIGIVPVNPFLRPGWRRSDSRQ
                     EVTLHHVVAQIDYICQLAGNARHVGLGTDYDGGFGLQSVPTGIETIADLQNLSPILAE
                     KGYTEDDTAAILGENWLSILRRGLPASS"
     gene            2968..3375
                     /locus_tag="AC16V7_17170"
     CDS             2968..3375
                     /locus_tag="AC16V7_17170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258909.1"
                     /translation="MGITILGLLIYLLGVDPALFGLDRSPVMGFVQIATLLVGLAILC
                     LSGFMTLNSLWNGSQKSISADIGLRLVATGYLVVVASSMADLLGFGNHTFPAIPYLGP
                     LQAIGVIIGEIAIVAGFIMMVPYPRRDQKTGLV"
     gene            3390..>5212
                     /gene="dnaE"
                     /locus_tag="AC16V7_17175"
     CDS             3390..>5212
                     /gene="dnaE"
                     /locus_tag="AC16V7_17175"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR6258910.1"
                     /translation="MFVHLHTHSYFSFLQGVPSPEELAQAAANAGMPAQALTDFNALS
                     GAVEYYDACRAAGVKPILGLEIDISPPPGLAAAPYQAGRLAFLAIDMEGWSSLCRMSS
                     ALLTEPDLEKRTVFPFDRLAEYAPGVLCLSGGSRGLAALFAQGGLEREAAALLGALSE
                     IFSKRLYVELQMHSRTDELWVQSLVSLAGRLDLPVVATHDVHYLNPDQAHLQRVVTAI
                     RLNKPLEEVMPDEQAPSEAYFLPEAEMVRRFADLPRALAATLEVAERCQLELPLGVPH
                     FPEVSFSQGETPDLILRKKAEMGAVHRYGEITPEIQHRLDHELAVIVDSGYSTLFLVM
                     EEILQYARKIGVPFSSRGSAASSLVAHCLGITSPDPLRLNLYFERFLNPARATPPDID
                     TDLCSRRRDEVIRFVYDRYGEQRVAMVCTINRFRRRSALREVAKAYGLSSSQINKLAE
                     ALPQRWYGPPPHGLRKENAFSELSERYTSSLHQAIFRDAAALLQKPRHLSIHPGGVVI
                     APRLLTDLVPTQMASKGVVVTQFDLEAIERMGLVKIDLLGIRGLTVLGDVAESINSAH
                     PGLYASPLSALDAIPDEDAATSEIVQLGRTIGCFQIESPGMR"
BASE COUNT         1076 a         1369 c         1500 g         1267 t
ORIGIN      
        1 cacgacagac gatccacgca atagtctcta tgtttttgat tataatggta tcaatgttgc
       61 ccatttgggg gatttgcgcc gtgttccctc tcagaccgaa atcgaagcac tgggtacggt
      121 tgacgtagct ttggtgcctg ttggtggtgg agggggctta aacgcagcca aagccgctga
      181 agtggtcagt ttgttggaac cggggattgt cattcccatg catttcagca cccctgccag
      241 cctgctcaaa cttgccccct tgagcaagtt cctgaaagag atggggctgg gtaaaactga
      301 acaacaggat tccctgaaag tcacgcggac atctgtgcct gaagagaccc gcgtggtggt
      361 gctgaattat gtgcgttctt gaataggaga cccatcatgt ctgttaaact cctaatgacc
      421 tgggatatcc ttccgggcag agaacaagag tattttgagt ttgtagtgcg agattggatt
      481 cctggcatcc agcgactggg tctggaacct agtgatgctt ggtttacaat gtacggcgac
      541 caacctcaaa tccttgccgc ggcgcaatca tccagcttaa gctctctgca gcacatattg
      601 aattctccgg attggaagac cctgacccaa cagctgctgg attacgtaga agatttcaat
      661 tacaaggtgg tatcggcacg gaccggtttc caattgtgag attggctgtg tgctgattgt
      721 tgggcttggc gtttggtcaa gaccaacaat cctgtgttat ggctttcctg tttaccgaaa
      781 accttctgga ctggtatgca aataacgccc gcgaccttcc ctggcgcggg cattgcgatc
      841 catacgccat ctgggtttca gagatcatgc ttcagcagac gcgggttgag acagttatcc
      901 cctatttcga gcgctggatg caacgcttcc ccacgattgc tgccctggca gcagccgacc
      961 agcaagatgt actggctgtt tgggagggat taggttacta tagccgggct cgcaacttgc
     1021 ataaggctgc cggagtggtt gttgatcaac atggtggagt gctgccatca gatgtgaccg
     1081 ctttacgacg gcttcccggc attgggcgct acagcgcggg cgccattgct tcgattgctt
     1141 ttgggcaaga cgagcccgcc ttagacggca atattcggcg tgtgctggcg cgctacttcg
     1201 atgtcagcga gccggctcgt gcgaccagcg gggagcgccg cctgtgggca ctggctgcca
     1261 tgcacctact tacgggacgc gctgcggatt ataaccaggc gataatggac ctgggcgcca
     1321 cgatttgtac ggcgcgcgct ccggactgcc cgcgttgccc cttgagggag ggttgtcgtg
     1381 ctcgcctcct gggtgtgcag gaagagcgac ctgtcctggc caagaagaca agccaaccgc
     1441 atcacattgt cactgcggca gtcatacgcc aggatgggcg gatattaatt gcccagcgac
     1501 ctgcacgcgg attattggga ggcatgtggg aattcccagg cggtaagctg cagcccggag
     1561 aagacctgcc aacttgcctg cagcgtgaga ttcgggagga attgggtgcg caaatcatgg
     1621 ttggtgggcg gctgggtgtg taccagcacg cttacaccca cttcagagtg accctgcatg
     1681 cctttgaatg ttccttgaag ggcgctagcc ttccgcaacc tcgagagcac gctggcctta
     1741 gatgggtcat tcctgctcag cttgtggatt atccgatggg gaagattgac cgtaggattg
     1801 caacccacat catcgaaagt gaagccgaat gttgatcgtc gatgcccacc aggatctggc
     1861 ctggaatacg ctaacctttg ggcgtgatta tacactgccc atttctgaaa cccgtcttcg
     1921 cgagcaaggt agtgtagctg tggaacacaa tggagataca ctgctgggct ggccggaata
     1981 ccagcgcggg caggtgggcg tgattttctc ttcattgttt gcctctccag cccgtctccg
     2041 ctcgggcgac tgggactccc aaacttatcg cgatttcatg gaggcgaatg cacgctatcg
     2101 cgcccaattg gacgtctacc accgccttgt tgaccaacac tccgataaat tccgtcttgt
     2161 gaaggggaac gatgatttgc aggaggtggt tgatcattgg aagcaaaaca cagcacagga
     2221 acatcccatc gggctggtca ttctcatgga aggtgctgaa ggcgtgcagg agccgtctga
     2281 gctgggggaa tggtgggatg gtggggttcg cttgatcgga cctgcctggg cgggtaatcg
     2341 ttattgcggc ggtacacgcg agcccggacc gctcacctcg gaaggatatg ctttactgga
     2401 tggtatggca gatctgggtt tcatcttgga tatcagccac atggatgaac aggccgcgct
     2461 acaggctctg gatcattacc cctcaacgat cgtggcgacg catgccaacg ccatcacctt
     2521 gctcaaaggc ttggatacca accgccacct cagcgatcgg gtaattcgta tgctgatcga
     2581 gcgtggcggt gtgatcggca ttgtccccgt gaaccccttc ttacggcccg gctggcgacg
     2641 cagcgactcg cgccaggagg tcacattgca ccacgtggtt gcccagatcg actatatctg
     2701 ccagctagcc gggaatgcca gacatgttgg cctgggtacc gattatgacg gtggattcgg
     2761 cctacaatct gtaccaactg ggattgaaac gatcgctgat ttgcagaatt tatcccccat
     2821 cttagcagaa aaggggtata ctgaagacga cactgcggcg atccttggag agaactggtt
     2881 atctatccta aggcgcggtt taccagctag ctcatgagaa taaataaatc tattaacgga
     2941 gtcaatcact ttcgcgtgcg attggggatg ggtattacca tccttggcct tttgatttat
     3001 ctgctcggtg ttgatccagc attatttggt ttggatcgca gtccggtgat gggctttgtg
     3061 cagatcgcta ctttattggt aggtctggcg atcctctgcc tgagcgggtt tatgacgcta
     3121 aattccttgt ggaatggatc gcaaaaatct atttcggcag atataggtct gcgcttggtg
     3181 gctaccggat acttggtggt agtggcatca agcatggctg acttgctcgg ctttggcaat
     3241 cacacttttc ccgcaatacc ctatttaggc ccattgcaag ccattggagt gatcattggt
     3301 gaaattgcca ttgtcgcagg cttcatcatg atggtcccat atccacggcg agatcaaaag
     3361 acaggcctgg tctagcttat tcgtctccgt tgtttgtcca tctccacacg cattcatatt
     3421 tctcctttct gcaaggcgtc ccttctcccg aagagttagc tcaggcagct gcaaatgctg
     3481 gcatgcctgc gcaggctttg actgatttca acgcgctgag cggtgcggtg gaatactacg
     3541 atgcttgcag ggcagcaggt gtgaaaccca tcttggggct ggaaattgat atcagcccgc
     3601 ctcctggctt ggcagctgcc ccttaccagg caggcaggtt ggcctttctg gcgatcgata
     3661 tggaggggtg gagcagcttg tgccggatga gcagtgcgct gcttaccgag ccagaccttg
     3721 agaagaggac cgtatttccg tttgaccgct tggcggagta tgctccgggt gtgctctgcc
     3781 tttcaggagg tagcaggggt ttggcagccc tgttcgctca aggcgggttg gaacgcgagg
     3841 ctgctgccct gttaggcgct ctgagcgaaa tattttcaaa acggctatat gtggagttgc
     3901 agatgcactc ccgcacagat gagttatggg ttcaatccct ggtttccctg gcgggacgac
     3961 ttgatttgcc tgtcgtggct acccatgacg tgcattatct caacccggat caggcgcatt
     4021 tgcagcgagt ggtcacagcg atccgcctca acaaaccgct tgaggaagtg atgccagatg
     4081 agcaggcgcc tagcgaggct tacttcctac ccgaggcaga gatggtcagg cgctttgccg
     4141 atctcccgcg cgccctggct gccacactgg aggtcgctga gcgatgccaa ctggaactgc
     4201 ccttgggtgt gccgcatttc cctgaagtct cattctccca gggggaaact cccgatctga
     4261 tcttgagaaa gaaagccgag atgggggcgg tgcaccggta tggcgagatt accccagaaa
     4321 tccagcaccg tctggatcac gaactggctg tgatcgttga ttccggttac tccacactgt
     4381 tcctggtgat ggaggaaatt ctccaatatg cccgcaagat tggcgtgccc ttctcttcgc
     4441 gcggttcagc ggcttcgtca ttggtggcgc actgcctggg catcacctcg ccagatcctc
     4501 tacgcctgaa cctgtatttc gagcgctttc tcaacccggc gcgcgccact ccgccagata
     4561 tcgatactga tctgtgctcg cgtcgccgtg atgaggtgat ccgctttgtt tatgaccgct
     4621 atggagagca gcgcgtggcc atggtgtgca cgatcaaccg tttccgccgc cgatcagcgc
     4681 tacgagaagt tgcaaaggct tatggcctgt catccagtca gatcaacaaa ttagccgagg
     4741 ccctgccaca gcgctggtat ggaccgcctc cacatggtct tcgcaaggaa aacgcctttt
     4801 ccgagctgtc ggaacgctac acgtcatcgt tgcaccaggc catattccgc gacgctgcag
     4861 cgttattgca aaagccgcga cacctctcca tacatccggg tggggtggtc atcgctccta
     4921 gattgctgac tgacctggta cctacccaga tggcgagcaa gggcgtggtc gtcacacagt
     4981 ttgacctgga agctatcgag cgcatggggc tggtgaagat cgacctgctg ggtatacgtg
     5041 gtttgaccgt tttgggcgat gtggcagaga gcatcaattc agctcatccc ggactttatg
     5101 cttctccctt aagcgcgctg gatgctattc cagatgaaga tgcagctacg tctgagatcg
     5161 tgcagctagg tcgtaccatc gggtgcttcc agatcgaaag cccaggcatg cg
//
LOCUS       DBNHOH010000953         2662 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1472212, whole genome shotgun sequence.
ACCESSION   DBNHOH010000953 DBNHOH010000000
VERSION     DBNHOH010000953.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1472212"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..135)
                     /locus_tag="AC16V7_17180"
     CDS             complement(<1..135)
                     /locus_tag="AC16V7_17180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S19 family protein"
                     /protein_id="HIR6258911.1"
                     /translation="MGRSLKKGPFIDPKLLKKVEVMNERGDKKVIRTWSRASTIFPQM
                     V"
     gene            complement(153..989)
                     /gene="rplB"
                     /locus_tag="AC16V7_17185"
     CDS             complement(153..989)
                     /gene="rplB"
                     /locus_tag="AC16V7_17185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIR6258912.1"
                     /translation="MAVKKYKPTTPGQRGMTGSTFEEITKKSPERSLLKPLRKSGGRN
                     LYGRITVRHRGGGHKRHIRIVDFKRDKRGVPAKVAAIEYDPNRTARLALLNYVDGEKR
                     YIIAPVDLKVGDTVLAGPGAEIRPGNSLPIANIPVGTMVHNIELKVGRGGQLVRAAGG
                     AAQLLAKEGDYAQVRMPSGEVRLVRQECYASIGQVGNLDHGNIKLGKAGRKRHKGIRP
                     AVRGSAMSPRDHPHGGGEGRQPIGMPGPKSPWGKPTLGYKTRRNKKTDQYIVRRRGKK
                     RR"
     gene            complement(989..1291)
                     /gene="rplW"
                     /locus_tag="AC16V7_17190"
     CDS             complement(989..1291)
                     /gene="rplW"
                     /locus_tag="AC16V7_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIR6258913.1"
                     /translation="MTSIYDVLRRPIVTEKSNYLNSDLHQYVFEVSSDATKHMVKDAV
                     ETLFDVDVVRVNIVNVPARRTRRIRSRRVLVRRGAYKKAIVTLAPEDSIDIFEGVK"
     gene            complement(1305..1937)
                     /gene="rplD"
                     /locus_tag="AC16V7_17195"
     CDS             complement(1305..1937)
                     /gene="rplD"
                     /locus_tag="AC16V7_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIR6258914.1"
                     /translation="MMEVDVLNMEGQKVSTVELPSLIFEAPVNVDLMHQAYVRQMANA
                     RLGTHKTKGRSEVSGGGRKPWRQKGTGRARQGSTRAAQWVGGGKIHTPKPRDYSMQMP
                     RKMRRAALRSALSVKAAESSIVVLDELSVSEPKTRLMAQALDQLVGDASVLILIPEST
                     TGYDMVIRSTNNLPDTKMLQANYLNIRDLLGYDKVLMPLKVLDVLVSFLG"
     gene            complement(1987..2619)
                     /gene="rplC"
                     /locus_tag="AC16V7_17200"
     CDS             complement(1987..2619)
                     /gene="rplC"
                     /locus_tag="AC16V7_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HIR6258915.1"
                     /translation="MLKGLIGKKIGMTQIFDDAGVAIPVTLIEAGPCYVTQVRLPEVD
                     GYSAVQLGFVEVKQKRLTSGQLGHLKRNNTPPLRFLREFRHKGPEVKEGDTLTVDAVF
                     NVGENVDVIGTSKGKGFAGSVKRHHFRGGPKTHGQSDRHRAPGSRGSGTTPGRVYKGS
                     RGAGHMGNERVTVQNLKVVLIDSERNLMGVRGAIPGPKGGIVMIKEARKR"
BASE COUNT          567 a          797 c          644 g          654 t
ORIGIN      
        1 caccatctgg ggaaagatcg tgctggcgcg actccaggta cgaatgactt tcttatcacc
       61 acgctcgttc atgacttcaa ctttcttcag cagttttggg tcaataaatg gccctttttt
      121 cagagaacgt cccataatct agtctcctgg tcttaccggc gtttcttccc tcggcgccgc
      181 acgatgtact ggtctgtctt tttattgcgg cgggtcttgt aacccagggt aggcttaccc
      241 cacgggctct taggacccgg cataccgatt ggctggcgcc cctcaccgcc tccgtgcggg
      301 tgatcgcgcg ggctcatggc ggagcctcgt acagctggac gaatgccttt gtggcgtttg
      361 cgccctgctt tacccagctt aatgttgcca tgatccaggt tacccacctg gccaatgctt
      421 gcatagcact cctggcgcac caagcgcacc tcgccagacg gcatacgcac ttgagcataa
      481 tcgccttctt tagccagcaa ttgggctgct ccgccggcag cgcgcaccag ttgcccacct
      541 cggcctactt tcagttcaat attatgtacc atcgtgccca ccggaatatt ggcaatgggt
      601 aggctgttac cgggacggat ctcagcacct gggcctgcta acacggtatc gccaacttta
      661 agatccaccg gggcgatgat atatcgtttt tcgccatcta cgtaattcaa gagagccaag
      721 cgagctgtgc gattgggatc atactcaatc gcagccacct ttgcgggcac gccgcgctta
      781 tcgcgcttga aatcaacaat ccgaatatgc cgcttatgac cgccgccgcg atgccgcact
      841 gtgatacgac catacaggtt gcgcccgcct gacttacgca gcggtttaag caacgagcgc
      901 tcaggcgact tcttggttat ttcttcaaat gtagatccgg tcatcccccg ctgacccggg
      961 gtcgtaggtt tgtacttttt tacagccatt acttcacccc ttcaaagatg tctatcgagt
     1021 cttcaggagc gagggtcaca atggctttct tgtaagcgcc gcggcgaaca agcacgcgcc
     1081 gactacggat tcgccgagtg cgccttgctg gcacattaac gatattgacc cgtaccacat
     1141 ccacatcgaa gagtgtctcc acagcatcct tcaccatgtg tttggtggcg tccgaagaca
     1201 cttcaaacac atactggtgt aagtcgctgt ttagataatt agacttttcg gtcacaatcg
     1261 ggcgacgcaa tacatcataa attgaggtca tctcaccctc ctacctatcc aagaaatgag
     1321 accaaaacat ccagcacctt gagcggcatg agtactttat cgtagcccag caagtcgcgg
     1381 atgtttagat aattggcttg cagcatcttg gtgtccggca gattattggt tgagcggatt
     1441 accatatcat aaccagtggt gctctctggg atgagaatca acacgctggc gtcacccacc
     1501 agttggtcaa gagcttgagc catcaagcga gtttttggct cagacacgct cagctcatct
     1561 aggacaacaa tcgagctttc agcagccttc accgaaaggg cagagcgcag ggcagcccta
     1621 cgcattttgc ggggcatctg catgctgtaa tcccttggct tgggagtatg aatcttgcca
     1681 ccgccaaccc attgagcagc tcgcgtcgat ccctggcggg cgcggccggt gcctttttgg
     1741 cgccaaggct tgcgaccacc gccggatacc tcgctgcgac ctttcgtctt gtgtgtcccc
     1801 aggcgcgcat ttgccatctg acggacgtac gcctggtgca tcaaatccac atttaccggg
     1861 gcttcaaaga tcagcgacgg caattctacc gtgctgactt tctgcccttc catattcaag
     1921 acatcaactt ccatgattcc atcgctccac tatatgacaa cccggttcga ggtgctatca
     1981 tgcaggctat cgcttacgcg cctctttgat catcacaata ccgcctttag ggccaggaat
     2041 ggcaccccgc acgcccatca ggttgcgctc actgtcaatc agcaccactt tgagattctg
     2101 cacagttacc cgttcattac ccatatgacc tgctccccgc gatcctttat agacgcggcc
     2161 tggcgtggtg cccgatccgc gcgatcccgg agctcggtgg cgatccgact ggccatgggt
     2221 ctttgggcca ccccgaaaat ggtggcgctt caccgaacct gcaaaacctt tacccttgct
     2281 ggtaccgatg acgtccacat tctcaccaac gttgaaaact gcatcgacgg tcagcgtgtc
     2341 gccttctttg acttccggac ctttgtggcg aaactcgcgt aaaaagcgca acggaggcgt
     2401 attgttgcgc ttgagatgcc caagttggcc agaagttagg cgcttctgct tgacctccac
     2461 aaatcccagc tgcactgccg aataaccatc tacctccggc aaccgaacct gggtgacata
     2521 acaaggccca gcttcgatta aggtaaccgg gatggcgaca ccggcgtcat cgaaaatctg
     2581 ggtcatgccg attttcttgc caatcagccc tttcaacatc tttttttctc ctaaatatca
     2641 agttttggga ctgtcagcct gg
//
LOCUS       DBNHOH010000954         3062 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1599166, whole genome shotgun sequence.
ACCESSION   DBNHOH010000954 DBNHOH010000000
VERSION     DBNHOH010000954.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1599166"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..532)
                     /locus_tag="AC16V7_17205"
     CDS             complement(146..532)
                     /locus_tag="AC16V7_17205"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HIR6258916.1"
                     /translation="MIQGQVARFFWGPFPTQCALGAIRQKFQFAQILNKEKISMSSML
                     VQHQVKNFAEWKKLFDSNIDLRKRSGELSAHVYRNESDPNNITALFKWDSLANAQKFA
                     HSPELKAAMEKAGVMGQPSIFFLNEA"
     gene            916..1818
                     /locus_tag="AC16V7_17210"
     CDS             916..1818
                     /locus_tag="AC16V7_17210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6258917.1"
                     /translation="MVNVLSDRDTPQSKATVLLVSDDLESARIWAYSLEQAGLQVCQA
                     GFSEKTLQVWSSEFPDLIILEDHNARADVFEFCRQIRQEAAVPILVMTSNSQEAHLME
                     AYRAGADDCVPPPVSPRLFMAKVKAWLRRAQVLPNAALAAVQVGEFQLDPDRRILTTP
                     NGKLLKLTNLETRLLYLFMNHPAWIMETDYLVNRVWGHFGDGDGILLKNLVYRLRRKI
                     EPNPSHPCYLLTESNLGYRFRPEGELSSTVDVPNKGQTGGSGQTQPVIHTTPLQARDS
                     AEGASRGRARDTGQLHLHYPKHND"
     gene            complement(1968..2204)
                     /locus_tag="AC16V7_17215"
     CDS             complement(1968..2204)
                     /locus_tag="AC16V7_17215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258918.1"
                     /translation="MTKKQAGQSAGFATGSAMTSPLSCEVRISDQVQSSRLRRHYVDM
                     DGIFIFFEGCLLCRSPEMALSLPGICLLARRSRR"
     gene            2388..>3062
                     /locus_tag="AC16V7_17220"
     CDS             2388..>3062
                     /locus_tag="AC16V7_17220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258919.1"
                     /translation="MTTHRINRLSLALALALTLSLLLASLGSAAVWSQDPESTPTPTP
                     TEAPTATEPPVEPAAPTETPTAEATQVLTPTHTITPTATQEPTPEATLVVTSTTTITP
                     TATLEPTQPPPAEPTPAPPLPPPTIASDLDDDPPGGWVTLTGQGWQSETEVRIIVEDY
                     YGSTWRRDEIVTVQENGSIRDYFQLPPWFVSDYVVLAIGQSTGATATTTFTDANVDNA
                     PITILKA"
BASE COUNT          679 a          927 c          858 g          598 t
ORIGIN      
        1 caacccctct taatcaggtc gggggattgc ttcgaacctg gaaagagcac caggttctcg
       61 cgatgacatg cgaattttgt ttggttggtt aaataaagct gcccctccgg agatccagaa
      121 gggcagcttc ttggtcatct aatttctagg cttcgttgag gaaaaagatg gagggttggc
      181 ccatcacgcc ggctttctcc atcgccgctt tcagctcggg tgaatgggca aatttttggg
      241 cgttcgccag cgaatcccac ttaaaaagtg cggttatatt gttcggatcg ctttcgttac
      301 gataaacgtg tgccgagagc tcgccgcttc ttttgcgcaa atcgatattt gaatcaaata
      361 actttttcca ctctgcgaag ttctttactt gatgttgaac taacatactg gacatgctga
      421 ttttctcctt gtttaagatt tgggcaaact ggaacttctg ccggatggcg cccagagcac
      481 attgcgtagg gaatggcccc caaaaaaaac gggcaacctg accttgaatc aagttacccg
      541 ggagggtggc ctaaatcagc agttgtatat caatttgacc ttactaatcc atattatacc
      601 acgggaactc attcgagaga tgatgattgg atgagtgttc aagggcaatc gcattagagc
      661 gatttccccc ctggatatgg ggaaaaagag tttccgaacg atctctttgt atacgcccac
      721 cccctcgatc catctccctg ccgcccttca ggctacgccc gcagggggtt aggggtgggt
      781 gggggttgtg gtgaggacgc ggcgaatctc caccctcaaa tactgtgact ttagtgtaac
      841 cagacaggtt aagagtcgtt taataggatg cacaaagcaa cccaaccttg tgcatcctat
      901 tcgtgagatt acccgatggt caatgtcctc agcgatcgtg acacgccaca aagcaaggcg
      961 acggtgctcc tggtgagcga cgacctggag agcgcccgca tctgggccta cagcctggag
     1021 caggctgggc tacaggtctg ccaggccggg ttcagcgaga aaaccttgca ggtgtggtcc
     1081 agcgagttcc ccgatttgat catcctggaa gaccacaacg cccgtgcgga cgtctttgag
     1141 ttttgccgcc aaattcggca ggaggcggcg gtgccgatct tggtaatgac ctccaacagc
     1201 caggaggcgc acctgatgga ggcctaccgg gccggggcgg atgactgcgt gccgccgccg
     1261 gtcagcccgc ggctcttcat ggccaaggtg aaagcctggc tgcgccgggc gcaggtgctg
     1321 cccaacgccg ccctggctgc cgtccaggtg ggcgaatttc agctcgaccc cgaccgccgc
     1381 atactgacca ctcccaacgg caagctgctg aagctcacca atctggagac ccgcctgctg
     1441 tacctgttca tgaaccaccc ggcctggatc atggagacgg attacctggt caaccgcgtg
     1501 tgggggcact tcggcgacgg ggacggcatc ttgctcaaga acctggtcta ccgcctgcgg
     1561 cgcaagatcg agcccaaccc cagccatccg tgctacctgc tcacagagag caacctgggc
     1621 taccgcttcc gcccggaagg ggagctctcc tcaacggtgg atgtgccaaa caaaggccaa
     1681 acaggggggt caggccaaac gcagcccgtg atccacacca ccccgctcca ggcacgcgat
     1741 agcgccgagg gcgcctccag gggacgggcg cgcgacaccg gccagcttca cctccactac
     1801 cccaagcaca acgattaacc cagcgccgcg cggtgcaaaa aatttgcgac cggctgtgac
     1861 cggttctgcg acgcatttaa cgacccccgt aggctatgat gaagtggaag gaatggggct
     1921 gaaaaccccg ccggggagcg ggttaaggct taacaaatgg cttgtattca gcgtctagac
     1981 cttcgggcta gcaggcaaat gccaggtagg gatagcgcca tctcaggaga tcgacacagg
     2041 aggcacccct caaaaaagat aaaaatccca tccatatcaa cgtaatggcg gcgtagacgc
     2101 gatgactgga cctggtcgct tatccgcacc tcacaagaga gcggtgaggt catcgccgag
     2161 ccagtagcga aaccggccga ttggccggct tgttttttcg tcatcgtgat gcatcgcaca
     2221 tttatttttc aaggcaatcc tgatctttat caagtatcac aatgacccag tctcggtttt
     2281 ggtttgagat tgcttcggtc gcccaaaggg ctccctcgca atgacctgaa cgagactctg
     2341 tttacaacat ctttcgaata cttacgtagc aattcttagg agatcaaatg acgactcaca
     2401 gaattaatcg attaagcctg gcgctggcac tggcgctgac cctgtccttg ttgctggcat
     2461 ccctgggctc ggcggcggtg tggagccagg atccggagag cacgcccacg ccgacgccca
     2521 ccgaggcgcc cacggcgacc gaaccaccgg tcgagccagc cgcgcccacg gagacgccca
     2581 ccgcggaggc gacgcaggtg ctgaccccca cccacaccat cacccccacc gccacgcagg
     2641 agcccacgcc cgaggcgacc ctggtggtga cctctacgac aaccatcaca cccacggcga
     2701 ccctggagcc cacgcagccc ccaccggcgg agcccacccc cgcgccgccc ctgcccccgc
     2761 cgaccatcgc cagcgacctg gatgacgacc cccccggcgg ctgggtgacc ctcacaggcc
     2821 agggctggca gagcgagacc gaggtgcgca tcatcgtcga ggattactat ggatcgacct
     2881 ggcggcggga tgagatcgtg accgtgcagg agaacggcag catccgcgac tacttccagt
     2941 tgccgccctg gttcgtgtcc gactacgtgg tgctggccat cgggcagagc accggcgcca
     3001 cggcgacgac cacgttcaca gatgcgaatg tggacaatgc gcccattaca atacttaagg
     3061 cg
//
LOCUS       DBNHOH010000955         2227 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1342930, whole genome shotgun sequence.
ACCESSION   DBNHOH010000955 DBNHOH010000000
VERSION     DBNHOH010000955.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1342930"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="AC16V7_17225"
     CDS             <1..1029
                     /locus_tag="AC16V7_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006448 - regulation of translational
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongator complex protein 3"
                     /protein_id="HIR6258920.1"
                     /translation="TLIDAHQYNETAAHRAVGLVVETRPDHVNPQEIAWLRYLGVTKI
                     QMGAQSLDDRILLLNQRGHSVDDTHQAVALLRAAGFKIVLHWMPNLLGATQDSDRLDF
                     TRLWDNLCPDEIKIYPTQLLANADLYAYWQRGEYHPYTTEELINLIADIKPSIPRYCR
                     VNRVIRDIPSTNVVEGNKRTSLRQDVHQEMKRRGTRCQCIRCREVRGQEVHPKKLRLD
                     EYIYSAGGAQEYFISYTTPDDKLAAFLRLSLPRDGSPETGLIDLEGAAIIREVHVYGQ
                     SLKVGAEQHGAAQHIGLGTRLLSHAEEIARSNGYSHLAVISAVGTRRYYTRRGFERGE
                     LYLVKPID"
     gene            1056..1574
                     /locus_tag="AC16V7_17230"
     CDS             1056..1574
                     /locus_tag="AC16V7_17230"
                     /inference="COORDINATES: protein motif:HMM:NF014471.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HIR6258921.1"
                     /translation="MSRWMSLFWIFIKVNLLTTSGPASVGLLYKESVGKLMTESQFVE
                     AVGFSSVLPGSDALQLAMFVGYSAGGVPGALAALLGSILPPTLIMLVIASLLQRLRGE
                     AWIGSFVSGLTPAVAVLMVLIAWQIFNSEKNGYGLVTMGIAGLSLVALLLKAPPPLVL
                     LGAGLMGVIFFR"
     gene            1571..>2227
                     /locus_tag="AC16V7_17235"
     CDS             1571..>2227
                     /locus_tag="AC16V7_17235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258922.1"
                     /translation="MSDSLLRLLLCTNGNENTRPALNYGVWMAGLLHSQVTLLGVIEE
                     LADEKIVKKLLDDTEEKLKDNDIPYDIRLDYGRGSVVVAKHASTGSFLTILGPLGRPA
                     WRRVIQGRSFRRVLEKVATPILYVRQVRPQLNKILLCLGGLEYSESVQQLCLYLAKIS
                     SASLTLLHVVEPVKLDYPISKEIHNHWQHILETDTPQGKTLRKAFEEAQSSGLPVQFK
                     V"
BASE COUNT          540 a          557 c          591 g          539 t
ORIGIN      
        1 actctgatag acgcgcacca gtataatgaa acagccgctc atcgcgccgt gggattggtc
       61 gtggagacac gccccgatca tgtcaacccc caggagatcg cctggttacg atatctgggc
      121 gtcaccaaaa tacagatggg cgctcagagc ctggatgatc gcatcctgct cctcaatcag
      181 cgcggccata gcgtcgatga cacgcaccaa gcggtggctc tcctccgtgc tgctggtttc
      241 aagattgtgt tgcactggat gccaaatcta ttgggcgcta cacaagattc cgaccgttta
      301 gatttcaccc gcttgtggga caatctatgt cctgatgaga taaagatcta tcccacccaa
      361 ctgctcgcca acgctgactt atatgcttat tggcagcgtg gtgaatacca cccgtacacc
      421 actgaagagc tgattaattt aatagcagat attaaaccca gcatcccgcg gtactgccgc
      481 gtgaaccgcg ttatacgcga tattccttct acaaacgttg ttgaagggaa caagcgcacc
      541 agcctgcgcc aggatgtcca ccaagaaatg aaacgacgcg gcactcgctg tcaatgcatt
      601 cgctgcaggg aagttcgcgg tcaggaagta cacccaaaaa agctccgttt agatgagtac
      661 atttatagcg ctggtggagc tcaggagtac ttcatctcat ataccacccc agatgataag
      721 ttggctgctt ttctgcgact ctcgctacct agagatggtt cacccgaaac ggggctgatc
      781 gacctggaag gagcggcgat catccgcgag gtccacgttt acggtcaatc attgaaagta
      841 ggggcagagc aacatggtgc cgcccagcac ataggcctgg gcacgcgtct gctttcacat
      901 gctgaagaga tagctcgctc taacggctac tcccatttag cagtcatctc ggcagtggga
      961 acaaggcgtt attacacaag aaggggattt gagcgggggg aattgtatct ggttaaaccg
     1021 atcgattaat ctacaaaaac aaaggagatc tccttgtgtc tcgctggatg agtctatttt
     1081 ggatattcat taaggtaaac ctgctgacta cctcaggccc tgcctcggtt gggttgttat
     1141 ataaagagtc agtcggaaaa ctgatgactg aaagccagtt cgtggaggct gtgggctttt
     1201 cgagtgtact gccaggcagc gacgctcttc aactggcgat gttcgtgggc tatagcgcag
     1261 gcggcgtgcc aggtgctctg gcagccttgc taggttcgat tctgccgccc acgctgatca
     1321 tgctggtgat agcttcgtta ctacagcgtc tgcgcggcga ggcttggatt ggcagttttg
     1381 tgagcgggct aaccccagca gtggcagtcc tgatggtgtt gatcgcctgg cagatcttca
     1441 acagcgaaaa aaatggttat ggtctggtaa caatgggtat cgcagggctg agcctggtcg
     1501 ctttgctgct caaagctccc cccccactgg tgttgctcgg tgctggcctg atgggagtga
     1561 tctttttccg atgagcgata gtctgctacg cttgcttttg tgtaccaacg gcaacgaaaa
     1621 tacgcgtccc gcactaaatt atggtgtgtg gatggcaggg ttattacact cccaagtcac
     1681 gctattgggc gtgatagaag agctagcgga cgaaaagatc gtcaaaaaac tccttgatga
     1741 taccgaagaa aagctaaagg acaacgatat cccgtatgac atccgtttgg attatggcag
     1801 gggatcggtg gtcgttgcca agcatgccag cacgggatcc tttctaacca tcttgggtcc
     1861 gcttggcaga cctgcctggc gtcgagtgat ccagggacgc tccttccgcc gtgtgttaga
     1921 gaaggtcgcc acacccatat tgtatgtgcg ccaagttaga ccgcaactca ataaaatatt
     1981 gctctgtttg ggaggtttgg aatactccga gagtgttcaa caactctgcc tctatttagc
     2041 aaagatcagt agtgccagtt tgactttgct gcatgtcgtt gagcccgtga aacttgatta
     2101 tccgatctcc aaagagatcc ataaccactg gcagcacatt ttggaaacgg atacacctca
     2161 gggaaagacc ctaaggaaag ctttcgaaga agcgcaatcc tcagggttgc cggtccaatt
     2221 taaagtt
//
LOCUS       DBNHOH010000956         2324 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1727985, whole genome shotgun sequence.
ACCESSION   DBNHOH010000956 DBNHOH010000000
VERSION     DBNHOH010000956.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1727985"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="AC16V7_17240"
     CDS             complement(<1..1163)
                     /locus_tag="AC16V7_17240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258923.1"
                     /translation="MSDKSTSSIKSVFNKGLVISIATWLLLSLVFGLAYTQSHLYNDN
                     QNTKFLHGLAMGGMGLLKEDWLANTADPLPAFSLLVSFTYAFLAERLFYVYFALLMGV
                     YAYSILGIASSLYGMNRSPAKYLAYFVVILAIHSIQGQIFARKVLDFDVEILHFGLAG
                     QYLLGLDFQNSSFGVFLLLSMHAFLQRKTVRAILWLSLASIFHSAYLFGAALMTLAYL
                     GDLFYENIRIENQTEYSPSPLTPLPKVGEEERSNKGVPEFAPHTLIQRVLIAAKAPFL
                     HGLLALVLVSPVVIYQQTALSSTSPQLSAQALDILVNQRIPHHTLPRVWMDGGAYLQI
                     AIMLPGLLLVRKTRLFPVMLALILGGALGWLIQVTTGSHSLAMLGPWRVSVILV"
     gene            complement(1153..1941)
                     /locus_tag="AC16V7_17245"
     CDS             complement(1153..1941)
                     /locus_tag="AC16V7_17245"
                     /inference="COORDINATES: protein motif:HMM:NF014409.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylyltransferase domain-containing protein"
                     /protein_id="HIR6258924.1"
                     /translation="MPEVSIAIIQARMASSRLPGKVLLDIGGQPMLSRVIERVKRART
                     VDRTVVATTTDPSDDPVERLCQERGYPCYRGSAQDVLDRYYQAARSHEAEIVVRITAD
                     CPVIDPGLIDETVKALFEPSPVSSQEPAIAYDFAANRLPPPWGRTYPIGLDVEACTFA
                     GLETAWVEARQPHQREHVMPFFYENPQRFRIRLLNHHTDLSAYRWTVDSAEDLELLRQ
                     IYSRFGGRDDFSWLEVLALFEREPELLQINAVVRHKDYREIDER"
     gene            complement(1944..>2324)
                     /locus_tag="AC16V7_17250"
     CDS             complement(1944..>2324)
                     /locus_tag="AC16V7_17250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258925.1"
                     /translation="VSMYPSLIFLVGMLTGVFYSTKLVKGDAGERRRALLNIFLLFYL
                     IVSFSLYVVFLNMYPLLDQGDTIKATYMLHVLVVLPILGAEFLEGLRARSPKAYLVSL
                     ALLGLVFAHNLPAMITRYKIFSIP"
BASE COUNT          565 a          615 c          672 g          472 t
ORIGIN      
        1 accaggatga ccgagactcg ccagggaccc agcatagcca ggctgtggct gccagttgtc
       61 acctggatga gccagcccag cgcgcctccc aggattagcg ccagcatgac cgggaataac
      121 cgtgttttac gtaccagcag cagcccaggc agcatgatgg caatttgcag atatgcaccg
      181 ccatccatcc acaccctcgg cagggtatgg tgtgggatgc gctggttgac caggatgtcc
      241 aacgcctgtg ccgagagctg cggagaggtg gagctcaaag cagtctgttg atagatcacg
      301 accggggaga ccaagaccag ggccagcaac ccgtgcagaa agggagcttt ggctgctatc
      361 aacactcgct gaatgagggt gtggggagcg aattcaggca cacccttatt tgacctctcc
      421 tcctccccta ctttgggaag gggggtaagg ggggatgggg aatactccgt ttgattttct
      481 atacgaatat tttcgtaaaa caggtccccc aaataagcta gtgtcatcaa ggcagcgccg
      541 aacaagtagg ctgaatggaa tatactggcc agggagagcc acaggatagc cctcacagtt
      601 ttccgttgca agaaggcgtg cattgagagc agcagaaaga caccaaaaga actattttga
      661 aaatctaaac cgagcaggta ctgacctgcc aggccaaaat gcaggatctc tacatcgaaa
      721 tccagcacct ttctggcaaa tatctgcccc tggattgagt ggattgccag gatcaccaca
      781 aagtaggcta ggtattttgc cggcgaacgg ttcatgccgt acaggcttga ggcgatcccc
      841 aggatgctgt aggcatacac gcccatcagc aaggcgaagt agacgtagaa gagtctctcg
      901 gcgagaaacg cataggtgaa gctgacgagc aggctgaagg ccggcagcgg atcggcggtg
      961 tttgccagcc agtcctcttt gagtagcccc atgccgccca ttgccagccc gtgtagaaac
     1021 ttggtattct ggttgtcgtt gtacaggtgc gattgggtgt atgccaggcc gaagaccagg
     1081 gagagcagca gccatgtggc gattgaaatc accaggccct tattgaaaac cgattttatg
     1141 ctcgaagtgc tcttatcgct catcgatctc gcggtaatct ttatgcctca ccacagcgtt
     1201 aatctgcaac agctctggct cgcgctcaaa tagggccagc acttccaacc aggagaaatc
     1261 gtctcgcccg ccgaagcggc tgtagatctg gcgcaatagc tcaagatctt cggcgctgtc
     1321 caccgtccag cgataggcgc tcaggtcggt gtggtgattg agcaggcgga tgcgaaaacg
     1381 ctgcgggttt tcgtaaaaga agggcatcac gtgctcgcgc tgatggggct ggcgagcttc
     1441 cacccaggct gtctctaatc cagcgaacgt gcaagcctcc acatccagac cgatagggta
     1501 ggttctcccc cagggcggag gcagccggtt ggcggcgaag tcgtaagcaa ttgctggttc
     1561 ctgactgctg actggtgaag gctcaaatag tgccttcacc gtctcgtcga tcagcccagg
     1621 atcgatcacc gggcagtcgg cggtgatgcg caccacaatc tcggcctcgt gggagcgagc
     1681 tgcctggtag tagcggtcga gcacatcctg ggcgctcccc cggtagcagg ggtagccgcg
     1741 ttcctggcac agacgctcta ccgggtcatc cgacggatcg gtcgtggtgg cgacgacagt
     1801 tcgatccacg gtgcgcgccc gttttaccct ttcgatgaca cggcttagca tgggctgtcc
     1861 gccgatatcc agtagcactt tgcccggcaa gcgggaagat gccatacggg cttgaatgat
     1921 ggcgatggat acttcgggca tacttaggga atagagaata tcttgtatcg agtgatcatg
     1981 gcaggcaaat tatgggcgaa aactagccca agcagtgcca ggctgacaag atatgccttt
     2041 ggggagcgcg ctcgcaggcc ttccaggaac tctgctccca ggattggcag aaccaccagc
     2101 acgtgtagca tataagtggc cttgatggta tcgccttgat ccagcagggg atacatgttc
     2161 aagaaaacaa catacagaga gaaagacacg atcaagtaaa acaacaggaa tatattcaag
     2221 agtgcccgtc ttctctcgcc agcatcgccc ttgaccagct ttgtggaata aaatacgccg
     2281 gtcagcatgc cgaccaaaaa gattagactg ggatacattg agac
//
LOCUS       DBNHOH010000957         3049 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_60529, whole genome shotgun sequence.
ACCESSION   DBNHOH010000957 DBNHOH010000000
VERSION     DBNHOH010000957.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_60529"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="AC16V7_17255"
     CDS             <1..343
                     /locus_tag="AC16V7_17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016046121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T7 exonuclease"
                     /protein_id="HIR6258926.1"
                     /translation="MSEMMNVEKAEGEKWHLIQTLAGDQTDGYSGVPGIGVKRAIALF
                     EEKGYSWKTVVEAFAEKDLSEDVALENARLAKILTASDYDFDKQQPILWSPAADYRVD
                     DGAGSKDETVK"
     gene            446..745
                     /locus_tag="AC16V7_17260"
     CDS             446..745
                     /locus_tag="AC16V7_17260"
                     /inference="COORDINATES: protein motif:HMM:NF023181.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HIR6258927.1"
                     /translation="MADSPAHYTRGAIEVWDFIRDQQLNYHLGYAIKYICRAGYKSSE
                     SKEKDLKKAIHYLENELKHTTLQVEQSERSSNSIPFSVWDPEQFGQPDYATGFDR"
     gene            723..1004
                     /locus_tag="AC16V7_17265"
     CDS             723..1004
                     /locus_tag="AC16V7_17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrophosphohydrolase domain-containing protein"
                     /protein_id="HIR6258928.1"
                     /translation="MQLDLIDEEYHEFRNAFYNEPYENELKELADLVYVCFQYAENME
                     WDLEEALDRVHRSNMSKLGLDGTPIRRADGKVLKGPNYQPPILNDLINP"
     gene            1001..2377
                     /locus_tag="AC16V7_17270"
     CDS             1001..2377
                     /locus_tag="AC16V7_17270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016071211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical enzyme family protein"
                     /protein_id="HIR6258929.1"
                     /translation="MTASYISRTGRVQSWIDDPTSRLPVSCTVFVVEDSITGDNGIEA
                     SWKFVSHALRYGAGCAVHLSKLRPKGTENDKGLVASGPVSFAKIYSTLNEILRRGGVY
                     KNGAVVCHLDLSHPDALEFITTSRSELPWVKRCINITDEWWEGCTFKEEILYGIKSGD
                     IWLNKVKYDNEGKRIRGNVCLEVYLPSRGTCLLQHVNLGACEFDDIPRAFTEGMSQLC
                     ELHGKTGVGESGEYLPSETDRQVGLGILGLANLLRRYGVTYDQFGRALEQFNKGESVR
                     SAAYELVTQINAGIESAASIARNNEMVRAFAIAPTASCSYRSTDLDGYTCTPEIAPPI
                     SQTVDRDSGTFGVQTYNYGDVEIASKVGWEAYKRVADGIMTLLNKTGLLHGYSFNSWS
                     DVVTYDEAFIQEWLESPQTSLYYSLQVMGDVQDKSSAYAALEESEVDDYLNSLLEDTP
                     EPQCDCAE"
     gene            2374..>3049
                     /locus_tag="AC16V7_17275"
     CDS             2374..>3049
                     /locus_tag="AC16V7_17275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016074578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HIR6258930.1"
                     /translation="MNPYQKLLERKRKWTPVQTTAGTCKAGAEEAIHRALALRHMELP
                     VGDFISNALNSEVPALAREVLVSNVKDEENHDIALGYIANAYGVDEKAEAEALRLKTA
                     WEAHPDHTITKALVAERAIFFVLLPFFRFNGDAGMRTVSADISRDEQIHVAVNSLAHT
                     ELGYNISPSLDKLRKATINWVMQPLGINTTDKYLDKKFWLDSSDRLMYEGKAPQLVET
                     KSARMPA"
BASE COUNT          864 a          736 c          726 g          723 t
ORIGIN      
        1 catgtcagaa atgatgaatg tggaaaaggc agaaggggaa aagtggcacc ttatacaaac
       61 attagcagga gatcaaactg atggttactc cggtgtaccc ggtattggtg ttaaacgtgc
      121 aatcgccctc tttgaagaaa aagggtattc ttggaagact gtcgttgaag cgtttgctga
      181 gaaagatctt tccgaagatg tcgcacttga aaatgcaaga ctcgcaaaga tcctcacagc
      241 atctgactat gacttcgaca agcaacagcc aattctttgg tcccccgccg ccgattaccg
      301 agttgacgat ggagcaggat ctaaagatga gacggttaaa tgaccttcta cctgaagcag
      361 gtaaggatga catcattact gtctttcttg cgctgcagaa acaaaacttt gtcctatcca
      421 ataccgtcag caatctagtc aaaaaatggc cgattcaccc gcccactaca cacgaggagc
      481 catagaggtt tgggatttta tccgagacca acaacttaat tatcatctcg gctatgctat
      541 taaatatatt tgcagagccg gttacaagtc ttctgaatcg aaagagaagg atcttaaaaa
      601 ggctatccac taccttgaaa atgaactcaa acacacaaca ctgcaagtcg agcagtctga
      661 gcgatcaagc aattcaattc cgttcagcgt atgggatcca gaacagttcg gacaaccgga
      721 ctatgcaact ggatttgatc gatgaagagt atcacgaatt ccgtaatgct ttttacaacg
      781 aaccgtatga gaacgagctg aaagagcttg cagaccttgt atatgtctgc ttccaatatg
      841 cggagaacat ggagtgggac ctggaggaag ctctcgatcg tgtccacaga agcaacatgt
      901 ctaagctagg attagacggc acacccatcc gacgagccga tgggaaggtc ttgaaaggac
      961 caaactacca gccacctatt ttgaacgacc ttatcaaccc atgaccgcat cttatatctc
     1021 tcgcacggga cgtgtccaat cttggatcga tgacccaacg tcccgcctac cggtttcgtg
     1081 caccgtgttc gtcgttgaag actcaattac cggagacaat ggcattgaag catcctggaa
     1141 atttgtatca cacgctctac gatatggagc aggctgcgcg gtacacctgt cgaaactgcg
     1201 acccaaagga acagaaaatg acaaagggct ggttgcatct ggaccggtat cctttgccaa
     1261 aatctattca acattaaatg agatcctccg tcgcggcggg gtgtacaaga acggagctgt
     1321 ggtgtgtcat ctcgacctta gccaccctga tgctcttgaa tttattacta cttctcgatc
     1381 cgaactaccg tgggttaaac gatgcatcaa catcaccgat gagtggtggg aggggtgtac
     1441 gtttaaagaa gagattctct acggtatcaa atcaggtgac atttggctca acaaagtaaa
     1501 atacgacaat gaaggaaaac gaatccgagg taacgtctgc cttgaggttt acctgccctc
     1561 acgaggtacc tgtctactcc agcatgtcaa tctcggtgcc tgtgaattcg acgacattcc
     1621 tcgcgctttc actgaaggta tgtcccagtt gtgcgaactc catggtaaga caggtgttgg
     1681 ggaaagcgga gagtacctcc caagcgaaac tgaccgacaa gtggggctcg ggatcctcgg
     1741 actcgctaac ctacttcgtc gatacggagt gacgtatgac caatttggac gtgcgttgga
     1801 acaattcaac aaaggagaat cagtacggtc tgcagcctat gaacttgtca cccaaattaa
     1861 cgctggcatt gagtctgcag ccagcattgc tcgcaacaat gaaatggttc gagcctttgc
     1921 tattgcgccc actgcctcct gcagttatcg aagcacagat ctggatggct atacttgcac
     1981 tccagaaatc gctccgccta tctcgcagac agttgatcgc gactcaggta ctttcggagt
     2041 acaaacatac aactatggtg acgtagagat cgcctctaag gtgggctggg aagcctacaa
     2101 acgtgttgcc gatggcatca tgactctact taacaagact ggacttctac atggttacag
     2161 cttcaattcg tggtccgatg tggtcacgta tgatgaagcg ttcatccagg agtggcttga
     2221 atcgccccag acttctcttt attatagtct ccaagttatg ggcgacgttc aagataagtc
     2281 aagcgcgtat gctgctctcg aagagagtga agtcgatgat tatctgaaca gcctacttga
     2341 ggacacccct gaacctcaat gtgattgtgc agaatgaacc cttatcagaa actactagaa
     2401 cggaaacgca aatggacacc ggtacagaca actgccggta catgcaaagc gggcgcggag
     2461 gaggcaatcc accgtgctct tgcattgcga cacatggaac tgcctgtggg agattttatc
     2521 agtaatgccc tcaattctga agtaccagcg ttggcacgcg aagtattggt gtcaaacgtc
     2581 aaagacgagg aaaaccacga catcgcactt ggttacatcg ccaatgctta cggtgttgat
     2641 gaaaaagctg aagcggaagc ccttcggctt aaaaccgctt gggaagcaca tccagatcat
     2701 acgatcacca aagcacttgt tgccgagcgt gcgatcttct tcgttcttct accattcttt
     2761 aggtttaatg gtgacgctgg tatgaggacc gtttcagcgg acatcagccg agacgagcaa
     2821 atccatgtgg cggttaactc actggcacac accgagctgg gttacaacat cagcccatcc
     2881 ctggacaagc tccgcaaagc taccattaac tgggtaatgc agccattagg tattaatact
     2941 accgacaaat atctggacaa aaaattttgg ctggattcta gcgaccggct aatgtatgag
     3001 ggcaaagccc ctcaacttgt cgaaactaaa tctgctagaa tgcccgcct
//
LOCUS       DBNHOH010000958         4160 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_253313, whole genome shotgun sequence.
ACCESSION   DBNHOH010000958 DBNHOH010000000
VERSION     DBNHOH010000958.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_253313"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="AC16V7_17280"
     CDS             complement(<1..373)
                     /locus_tag="AC16V7_17280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005578658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HIR6258931.1"
                     /translation="MYDRQKLAKLKQELEKWEETSLQRSLSQLSERNDRYMTTSSEDI
                     ERLYTPLDVAEMDYLRDLGLPGEYPYTRGVHPTMYRSRLWTMRMFAGFGTAEETNSRF
                     KYLLNQGQTGLSIAFDLPTLMG"
     gene            complement(402..4031)
                     /locus_tag="AC16V7_17285"
     CDS             complement(402..4031)
                     /locus_tag="AC16V7_17285"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR6258932.1"
                     /translation="MAVVTLWPLFSIYLGHFAVYFPETGARTPRLALDLFSNTTELEA
                     RARLQISLLRTISQISQLIGKTSDPQILLERACQYLVSNRAYSLVWIGMVHPARDGSQ
                     LEIHPAARAGRGRHIIEDIHTSRKGVSERSNPAETAAQSGQAFLIQDIAQTTQDAPWQ
                     EAALQYGFLSSAALPMRHAGHTLGVLNIFGEGPNAFDREETDLLQELADELAHALISL
                     EARQQQAILHTAVETMQDGLFITNIEGRILYANSAIAQLLEVSSQELEGLDIRRVFPT
                     NGQFLPFDKLRLAIERDEKADIDLELRLSDQRNVYFSIRASLAMDTISQPAYVVINLR
                     DTTRHHQYEHQLLTLNRFTTELVQAHEVNILMDRLFVAGESLLQADASSFFLLQGDNH
                     SIAQSFTHNLPALKLENLTSLNELLPDTIPYPTRQPIVIAEPQDKPGYAARLENLTGG
                     MQTLLLLPISLQDRQIGALSFFYRQVRRFTEEEIQLGLTLAHTLAISLENTRLYQAEK
                     SQRDFAEALARGAAALNNSLEFDRVLDQILDQTLDVVQCRSVNIMLIEGDQVRVVRHL
                     DKSDPSEAKRPVTGKALSLLLPTLQQMLSSGKPLVIPNTSQDPLWQQLEQTTWIRSYA
                     AAPLQVQGRVIGFLNMNSDEVGYFTAQIFPRLQAFADTSAAAIQNASLYKESQMRGEE
                     LATLVEAAAVVSSSLDVNQVLQVVAEQMISLLNIRGCAISDYDPETKSVKLISDFGPI
                     EWLNNKERDKPYYLDEYPLTRQVLVTGEPLLVRVDDPDLEANERGFMERNHIKVSLML
                     PLVTRDRTIGLVELVDDRLDHSFSESEIELVQTLASHAAIAIENARLYQHTQKYAEEL
                     EDRVRERTMELRNAKERIEHILVSVPDAIIVLDEDNRLIQANPSGDALIVQANEHGLD
                     IFNSDFLRSLARQNTPTEKTILQLRDRAYQALASSLPIDEKKFGTVIVFRDVTRFREI
                     DQMKTQFVSDVSHELRTPLTNLSLYLDLLATTNDTAKNQRFLATLRRETQRLTSLIED
                     LLTISRLEADRVEINIEPVNLNHIVSDLAEDRRQMAANLGLNLTYTTHPELPYALADT
                     RLLTQVLSNLLTNALNYTPPSGHIHLQTLMQDWNDETWITVEVTDNGLGIPPEELEHL
                     FTRFFRGFASQESSAPGTGLGLAISKEIIDRLNGRITVKSQPNQGSTFTVWLQPIQSH
                     SGLSD"
BASE COUNT          921 a          991 c         1193 g         1055 t
ORIGIN      
        1 agcccatcag tgtgggcaga tcgaaggcga tcgacaggcc ggtttggccc tggttgagca
       61 aatacttgaa ccgggagtta gtctcttcgg cagttccaaa tccagcaaac atccgcatcg
      121 tccacaagcg gctgcggtac attgtcggat gtacaccgcg tgtatagggg tactcgccgg
      181 gaagccccag gtcgcgcaag taatccatct cggctacatc aagcggtgtg tagagacgct
      241 cgatgtcttc cgaagaggtt gtcatgtagc ggtcgttgcg ttccgaaagc tgtgataggc
      301 tccgctgaag ggaggtttcc tcccacttct ctaattcttg ctttagcttc gctagtttct
      361 ggcggtcgta catgctatct ccttcgctct gtgacgatgt tttaatcgct taatcctgaa
      421 tgcgattgta tcggctgtag ccagacagtg aaagtgctgc cttggtttgg ttggctcttc
      481 actgtgatac gcccgttgag gcgatcgata atctctttcg agattgccag ccccaatccg
      541 gttccaggag cgctgctttc ctggctagca aaaccgcgga aaaagcgcgt gaagagatgt
      601 tccaattctt ctggcgggat gcccaggcca ttatcagtta cttctacagt gatccaggtt
      661 tcgtcgttcc aatcctgcat gagagtttgt aagtggatgt gccctgaggg aggggtgtaa
      721 tttagtgcat tagtgagtag attagaaaga acttgagtga gcaggcgtgt atcggccagg
      781 gcgtagggca gttctggatg tgtagtgtat gtcagattca gccccaagtt agctgccatc
      841 tgcctgcggt cctcagccag atcggaaaca atgtgattga gattcacggg ttcgatattg
      901 atctctaccc gatcggcttc gaggcgcgaa atggtcaaga ggtcttcaat taatgaggtc
      961 aagcgttgag tctcacggcg caacgtggct agaaagcgtt gatttttagc tgtgtcattt
     1021 gtggtggcga gcaaatccag gtaaagcgaa aggttagtta gcggggtgcg cagctcatgt
     1081 gagacgtctg atacaaactg ggttttcatc tggtctattt cgcgaaaacg ggtgacatcc
     1141 cgaaagacga taacggtgcc aaatttcttt tcatcaatag gtagggatga ggccaaggct
     1201 tgataagcac gatctcgtaa ttgcaggatc gtcttttcgg ttggggtatt ctgcctcgcc
     1261 agactacgta aaaagtcgga gttaaaaata tccaaaccat gttcgttagc ctgtactatc
     1321 agagcatcgc cggatggatt ggcttggatc aggcggttgt cctcatccag cacgatgatg
     1381 gcatcaggaa cagagaccag gatgtgttca atgcgctctt tggcattgcg cagttccatg
     1441 gtgcgttcac gcacgcggtc ctcaagctct tccgcgtatt tctgggtatg ctggtagagg
     1501 cgggcgttct cgatggcgat cgctgcgtgc gacgccaggg tttgcaccag ctcaatctcg
     1561 ctctcggaaa aactgtggtc caggcgatca tcgactagct cgaccagccc aatcgtccgg
     1621 tccctagtta ccaatggaag catcagcgac acttttatgt ggtttcgctc catgaacccg
     1681 cgctcgttcg cttcaagatc agggtcatca acacgcacca gaagcggctc ccctgttacc
     1741 agtacctgac gtgttaatgg atattcatct agataatatg gcttatctct ttccttgttg
     1801 ttcagccact caatgggccc aaaatccgat atgagcttga ccgatttggt ttcaggatcg
     1861 taatccgaga tcgcacaacc tctgatattc agcaggctta tcatttgctc agccactacc
     1921 tggagaacct ggttgacgtc caggctggag gagaccactg cagcggcctc taccagggta
     1981 gcaagttcct ctccgcgcat ctggctttct ttatacaggc tggcgttctg gattgccgcg
     2041 gcagatgtgt ctgcgaaggc ttgcaagcgg gggaagattt gggctgtgaa gtagcccacc
     2101 tcatcgctgt tcatgttcaa gaagccaatc acgcgaccct ggacttggag cggcgcggca
     2161 gcatatgaac gaatccaggt ggtctgctca agctgctgcc acaagggatc ctgagatgta
     2221 tttggtatca ccaggggttt gccgctgctg agcatttgct gcaatgtggg tagcaacagg
     2281 gaaagcgctt ttcccgtcac ggggcgtttg gcttcgcttg ggtctgattt atccagatgg
     2341 cggacaaccc gtacctgatc gccttcgatc agcatgatat tgactgaacg gcattggacg
     2401 acatccagcg tttgatcaag gatttggtcg agtacccgat cgaattccag gctgttattt
     2461 agtgctgctg ctccgcgcgc cagtgcttcg gcgaagtcgc gctggctttt ctcggcctga
     2521 tacaggcgcg tgttctccag ggagattgcc agcgtgtgcg ccagggtgag accaagctgg
     2581 atttcttcct cggtgaatct gcgcacctgg cggtaaaaga acgataaagc cccaatttgc
     2641 ctgtcctgca gcgagatggg caggagcaac aaggtctgca tgccccccgt taaattttct
     2701 agtcgggctg cgtatcctgg cttatcttga ggttcggcta taacgatggg ctgtcgggtt
     2761 gggtagggga tcgtatcagg cagcagttcg tttaagctag ttaggttttc cagtttaagg
     2821 gcaggcaggt tgtgggtgaa gctttgggca atggaatgat tatcaccttg caagagaaag
     2881 aaactgctgg cgtcagcctg tagtaaggat tcgccggcga caaaaaggcg atccatcagg
     2941 atattcactt catgagcctg gacaagctcg gtcgtgaaac gattaagcgt aagaagttgg
     3001 tgttcgtatt ggtggtggcg agtggtatct cggagattaa tcaccacata agcaggttgg
     3061 gagattgtat ccatggctaa tgacgcccgg atagaaaaat atacattgcg ttgatctgag
     3121 agccttagct ctaggtcaat atcggctttc tcgtctctct ctatggctaa ccgtagcttg
     3181 tcaaaaggca ggaattgtcc gttggttggg aataccctgc gaatatctag gccttctaat
     3241 tcctgggagc tgacctcaag cagctgcgcg atcgccgagt tggcgtagag aattcgtcct
     3301 tctatattgg tgatgaatag accgtcctgc attgtctcca ctgcagtgtg caggatggct
     3361 tgctgctggc gcgcttcgag gctgatcagg gcgtgtgcta gctcgtccgc cagctcttgc
     3421 aggaggtccg tttcttcacg gtcgaacgcg tttgggcctt ctccgaaaat attgagcacg
     3481 cccagggtat gcccggcatg ccgcataggg agagcggctg atgagagaaa tccatattga
     3541 agggcagcct cttgccaggg ggcgtcttgt gtggtttggg cgatatcttg gattaagaag
     3601 gcttgtccac tctgggcagc tgtttcagcc gggttgctac gctcagatac tccctttcgc
     3661 gaggtgtgga tatcttcgat gatgtgacgc ccccgtccgg cacgcgcggc tggatggatt
     3721 tcgagttggg agccatcacg cgccgggtgg accatgccga tccacaccag tgaataagcg
     3781 cggttggaga cgagatattg gcaggcgcgt tccagtaaga tttggggatc gcttgtcttg
     3841 ccaatcagtt ggctaatctg gctgatggtt cgtaaaaggc tgatttggag gcgggcgcgc
     3901 gcctcaagct cggttgtgtt ggaaaacaag tccagagcta ggcgaggcgt cctagcaccg
     3961 gtttcaggaa agtaaacagc aaaatggcct aaatagattg agaacagagg ccataaggtg
     4021 acgactgcca acgtattgcc ccccaaaaga ataagaatca aattggctag aatgggcgcg
     4081 ctagaagagg taagcagtga aaacaaaatt gcatccaacc agacggctgc cagcaacccc
     4141 aatgaagctg ctgcacgact
//
LOCUS       DBNHOH010000959         9068 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1407408, whole genome shotgun sequence.
ACCESSION   DBNHOH010000959 DBNHOH010000000
VERSION     DBNHOH010000959.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9068)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9068)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9068
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1407408"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="AC16V7_17290"
     CDS             <1..662
                     /locus_tag="AC16V7_17290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258933.1"
                     /translation="YLTSTANVARAQQETPTPSPSEVPTGTPISPRPLGTPELSIRSP
                     LPGQAVQGSVPIAGSSAVTGFVSAEVSFTYAGDQTGTWFFIGDTNDAVVDGTLAQWDT
                     SSITDGDYDLRLRVLREDGSYLDAVILGLRVRNYTPIETATPTPVTPTATQMPGELTA
                     TSTAIPMSLPSVTPLPTNPAEITGQDIAWGLGKGALAVIGLFALMGVYKLVNEIGNKE
                     Q"
     gene            683..1303
                     /gene="pth"
                     /locus_tag="AC16V7_17295"
     CDS             683..1303
                     /gene="pth"
                     /locus_tag="AC16V7_17295"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR6258934.1"
                     /translation="MTSQRSLDMNQPATFLIIGLGNPGREYQGNRHNIGFRLLNHLAE
                     RLGVGFSRLESKSLVTKADYLGHRLILAKPQTYMNLSGQAVESLVRFYKIPLGQVMVV
                     FDDVDLPFGALRLRPAGGSGGQKGMQSILDRLGTQEIPRLRLGIDRPPGRMEAADYVL
                     QDFSKNEVELLPGILDRAADAVLTYVRNGLDAAMNQFNPQNSDDQG"
     gene            1308..4727
                     /gene="mfd"
                     /locus_tag="AC16V7_17300"
     CDS             1308..4727
                     /gene="mfd"
                     /locus_tag="AC16V7_17300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIR6258935.1"
                     /translation="MSMQLDAILNTVMETLGQRTSYLLGQVLPSFDLRRAARLPVLVS
                     MYRMTNQPVLLITDRADHALTLFDELSLWAPNAKRLLIPEPTSLFYEKAPWGENTRRE
                     RLVALTTLAAYHVPGTTPSVNDSLNHAILVAPARALMARTLPLREFLKSTRSLRPGQN
                     AQPDELARAWSTLGYTHVNTVIAPGQFSRRGGILDIWSPASEHPSRIEFFGDEIETLR
                     QFDPATQRTSGHSERLMITPAREYILPTDLETPPEEISEFHIPIMHPYPASLLDYLPR
                     QALVLIDDLQAILDATLDLEEQAVTFRKDAIAEGVIPEDFPVPYLSWSEIADNLPADR
                     TMVLGSKTDLETLQDEDSQSGNPISRSFLPGPRYGGRLKPFMEYLTERVEQGENSIIV
                     SRQSARLAELWGESTPYPLPTSPIFIEGSLTEGFTFLSADSPPVHLFTDGEIFGWRRP
                     ELRQRPRAVAESPEGAYTDLQAGDYVVHVDHGIGIFVGLVTRTIDSLEREYLCIEYAD
                     EAQLFVPVHQADRVTRYVADTRTPALSRLGGMEWRNVKTHVKEAVVEVAQDLLELYAT
                     RSVVKGHAFAKDSPWQQELEASFPYIETDDQIRVLDEVKRDMETARPMDRLICGDVGY
                     GKTEVALRAAFKAVMDGKQVAVLVPTTVLAQQHYQTFRQRLAAFPVAVEMLSRFRSHA
                     EQRDIVQRLQNDAVDIVIGTHRLLSSDVVFKDLGLLIIDEEQRFGVTHKETLKKLRSE
                     IDVLTLTATPIPRTLYMALTGVRDISTINTPPEERLPIITHVGPYQPRLVRQAILREL
                     ERGGQVFFVHNRVQTIGGISRHLEQLVPEARIAIAHGQMPEHILSERMKQFVNGDVDV
                     LVSTSIIESGLDIPNANTLIVDRADSFGLAQLYQLRGRVGRGAQRAYSFFFRHTRRSP
                     TPEGRERMETIAENTQLGAGYSIAMRDLEIRGTGDILGTRQHGHIVAVGFHLYTRLLA
                     EAVRQLRPSQPALLQKGWHIPLPSPANVDLPMPANIPAKYVADRTMRLRLYRRMADLQ
                     TLQEVDSLADEFVDRFGPLPVAVSNLFFQLKVKLLAEQASLASISSENGQLVMRFPDK
                     EVPASLPNPGPFVRVGKTALWMPYTNLPNWRETLVEVLKNLANKDGVAIRE"
     gene            4774..5400
                     /locus_tag="AC16V7_17305"
     CDS             4774..5400
                     /locus_tag="AC16V7_17305"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR6258936.1"
                     /translation="MFPPGIPLSKRLLDLLLTIPGLVLVSPVLLLIALMVRIIHGKPV
                     LFRQLRPGYRGQPFYVYKFRTMTEDHDEVGNLLPDTHRLTALGRFLRTTSLDELPELF
                     HVLAGKMSLVGPRPLLMQYLDLYTPEQARRHDVLPGITGWAQVNGRNAITWEDKFRFD
                     VWYVDNWSLWLDIKILAVTLWKVLKREGISQPGRATADEFMGSKRDEG"
     gene            5403..6299
                     /locus_tag="AC16V7_17310"
     CDS             5403..6299
                     /locus_tag="AC16V7_17310"
                     /inference="COORDINATES: protein motif:HMM:NF012880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR6258937.1"
                     /translation="MMENTALYRSLYLIRAFEETVLANFPRGIFFGTTHTYLGQEANA
                     VGVLSHLQAGDVVFSNHRCHGHYLAYGGDPRGLFAELMGKATGVCGGRGGSQHLHWKN
                     FYSNGVQGGIVPIATGMALAEKRKGSGAVTVAFLGDGTLGEGVVYEALNMASLWQAPI
                     LFVLENNRIAQTTPVELAVAGEIPARFNAFGIPARQLDSSDVNEIVPLAGELMKEVRT
                     LSVPRALILHTYRFGPHSKGDDTRNPQDVDRIKQARDPLSIHAPRLGPEERAAIEADI
                     KQLIEQAFHQAMVDPFPVISDA"
     gene            6328..7404
                     /locus_tag="AC16V7_17315"
     CDS             6328..7404
                     /locus_tag="AC16V7_17315"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR6258938.1"
                     /translation="MNTVLETLNSALHRAFTDDAHVLLLGEDVLDPYGGAFKVTRGLS
                     TRFPERVLTTPISEAGIVGVAAGMALRGLRPVVEIMFGDFVALAADQLVNHLAKFRWM
                     YNEQVRLPLVIRTPMGGRRGYGPTHSQTLEKLYLGLPGLRVIAPTALGNPGDLLYDMI
                     LHTGDPVLFIENKLLYAMPLHGEDLVEDFIPYPLSPIPSEIGGERQGIGDHSNPTYGL
                     SLRGAPPPQLTITAYGYMAELALQAVRRLAYQHEIFVELIVPTELSAFDLEPLFSSLE
                     RTHRLLTVEEGTYTLGWGAEVVARAAEKLGPRLHASRRLAAQDLPIPASGPLESAVLP
                     GVDEIISVALKLSTGKDRKHDLSR"
     gene            complement(7638..8243)
                     /locus_tag="AC16V7_17320"
     CDS             complement(7638..8243)
                     /locus_tag="AC16V7_17320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR6258939.1"
                     /translation="MNPGANSKELPRMARWLAILLVLSLLVSMLPQGVLAAPLDAKDD
                     CKRSYEVTRTDTLKKIARRFGVSVATIVAENDMKKPYTIYIGQRLCIPKRTKKGLSNL
                     PSKNTNTYAVFFVASRAGNDIIIYTYNYPKTTVLVKGENAGDSKWKLVDIGKINIATQ
                     GNRKAIRFKLPSELRVKKLMICLKDKSSGNLQCVYQRFSSK"
     gene            complement(8369..8854)
                     /locus_tag="AC16V7_17325"
     CDS             complement(8369..8854)
                     /locus_tag="AC16V7_17325"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR6258940.1"
                     /translation="MTTKQWNTAPEMQIDPKKSYVIKMETNKGLIKLELYPQHAPKTA
                     NNFVFLAREGFYDGVTFHRVIANFVIQGGYPTGTGRGGPGYRFEDETRGNPLRHETNV
                     ISMANAGPNTNGSQFFITYSPQPHLDSKHTVFGKVVAGNDVVNAIEQGDQMVKVEVEE
                     S"
BASE COUNT         1939 a         2450 c         2481 g         2198 t
ORIGIN      
        1 tgtatttgac gtccactgca aatgttgccc gagcccaaca ggagacgccc accccatccc
       61 catcggaggt accgactggg actcccatct cgccgcgccc tctgggaact cccgagctgt
      121 ccatacgttc tcccctgcct gggcaggcag tacaaggcag cgtaccaatc gccggaagca
      181 gcgcggttac aggttttgta tctgccgaag tatcttttac ctatgcaggt gaccagacgg
      241 gtacctggtt ctttattggc gacaccaacg atgcggtggt ggatgggacg ttggcgcagt
      301 gggataccag ctctatcacc gatggcgatt acgacctgcg tttaagagtc ctgcgtgaag
      361 acgggagcta tcttgatgca gtcatcttgg gcttgcgcgt gcgcaactat acgccaatcg
      421 agacggctac accaacaccc gtcacgccca ctgccacaca gatgcccggc gagctgaccg
      481 cgacatctac ggccataccc atgagtttgc cctctgttac gccgctgcca accaaccctg
      541 ctgaaatcac aggccaggat attgcctggg gtttgggtaa aggtgcgctg gctgtgattg
      601 gcttgttcgc cttgatgggc gtttacaagc tggtaaatga gattgggaac aaagaacagt
      661 aattattgaa tggccttatc gtatgactag tcagcgctct ttagacatga atcaacctgc
      721 aaccttcctg atcattggct tgggcaaccc aggtcgcgag taccaaggca accgccacaa
      781 tattggcttc aggctgctga accatttggc ggagcggtta ggtgtgggat tctctcggct
      841 cgaatcaaaa tcgctggtga cgaaggctga ttaccttggt catcgattga tactggccaa
      901 gccgcagacg tatatgaacc tatctgggca ggcagttgaa tcgctagtgc gtttttataa
      961 aatcccgctg gggcaagtga tggtggtatt tgacgatgtt gacctgccat ttggagcgtt
     1021 acgcttgcgc cctgcaggag gatcaggcgg gcaaaaaggg atgcaatcaa tcctcgacag
     1081 gttgggtacg caggaaatcc ctcgactgcg attgggaatt gacaggccac cgggtcgcat
     1141 ggaagcggcc gattatgtgc tgcaggattt ctcaaaaaat gaagtcgagt tgttacccgg
     1201 catattggac cgggctgctg atgcggtgtt aacctatgtt cgcaatgggt tggatgcagc
     1261 catgaaccag ttcaacccac aaaattcgga tgatcaagga taggtgaatg tcgatgcaac
     1321 ttgatgccat cctcaatact gtgatggaga ctttaggaca aaggacatcc tatcttctag
     1381 ggcaggtgct gccaagtttt gatctgcgcc gagcggcgcg cttaccagtc ttggtatcca
     1441 tgtacaggat gaccaaccag cctgtcttgt tgatcaccga ccgggcggat cacgctctga
     1501 cattgtttga tgaacttagc ctgtgggcgc caaacgcaaa gcgcttgtta attcctgagc
     1561 cgacttcgct cttctacgag aaagcgcctt ggggcgagaa cacccgtcgc gagcggctgg
     1621 tggctttgac caccttggca gcataccatg tccctggcac taccccttca gtaaatgata
     1681 gcctgaatca cgccatcctt gtggcgccag cccgtgcctt gatggcgcgt acgcttcccc
     1741 tgcgcgagtt cctcaaaagc acgcgaagcc ttcgcccagg acagaatgcc cagcccgatg
     1801 aactggcgcg cgcttggtct actctgggtt atacacacgt caacacagtc atagccccag
     1861 gtcagttttc ccgccgcggc ggtattctcg acatctggtc tccagcgtcg gagcatccta
     1921 gccgaatcga gttttttggc gacgaaatag agaccctgcg acaatttgac ccagctactc
     1981 agcgaacatc cggccatagc gagcggctga tgattacccc agcgcgcgag tatatcttgc
     2041 caactgacct ggagacaccc ccagaagaaa tctccgagtt tcacatacca atcatgcatc
     2101 catacccggc tagcttgtta gattacttgc ctcgtcaggc gctggtgctt atcgatgatt
     2161 tacaggcaat actggatgcc actttagacc tcgaagagca agcggtaact ttcaggaagg
     2221 acgcaattgc cgaaggcgta atcccagagg atttccccgt cccctacttg agctggagtg
     2281 agatcgcgga caacttgcct gccgatcgca cgatggtctt ggggagcaag accgatctgg
     2341 agaccttgca ggacgaggat tctcagtctg gcaaccctat atcccgcagt tttcttcccg
     2401 gaccacgcta tggtggcagg ctgaaacctt tcatggagta cttgactgag cgagtggaac
     2461 aaggtgagaa ttccatcatt gtctcacgcc agagcgctcg cctggctgag ttatggggcg
     2521 aaagcactcc ttatccactt ccaacttctc caatttttat tgagggaagc ctgacagaag
     2581 gttttacttt tctatcagct gattcgcctc ccgtgcactt attcacagat ggtgagattt
     2641 tcggctggcg acgcccggag cttcgccagc gcccgcgcgc agtggctgaa tccccagagg
     2701 gtgcatacac tgacttgcag gctggagatt atgttgttca tgtggatcac ggcatcggta
     2761 ttttcgtggg cctggtaacg cgcacgattg attcattaga gcgcgaatac ctgtgcatcg
     2821 aatatgccga tgaggcgcag ttgtttgtgc ctgtgcatca ggcggatcgc gtcactcgtt
     2881 acgtcgccga tacgcgcacg ccggcgctga gccgcctggg ggggatggaa tggcgcaatg
     2941 tcaagactca tgtgaaagaa gcggtggtag aggtagccca ggacttgctt gagctttacg
     3001 caacgcgcag tgtggtgaag gggcacgcct ttgcgaagga ttctccctgg cagcaagaat
     3061 tggaagccag cttcccctat atcgaaaccg acgaccagat tcgcgtgctg gacgaggtga
     3121 agcgcgatat ggaaaccgcg cggccgatgg atcggttgat ttgtggcgac gtgggatatg
     3181 gcaagaccga agtggcttta cgcgctgcct tcaaagctgt gatggatggc aagcaagtcg
     3241 cggtgctggt tcccaccacg gtactggccc aacagcatta ccaaaccttc cgccagaggc
     3301 tggcagcctt tccggtggcg gtggagatgc tctctcgctt ccgctcccac gcagagcagc
     3361 gggatattgt gcagcgcttg cagaatgatg ccgtcgatat cgttattggt acgcaccggc
     3421 tcctgtcttc agatgtggta ttcaaagacc tggggctcct gatcatcgat gaggagcaac
     3481 gctttggtgt gacccacaaa gaaacgctca agaaattgcg ttccgaaatt gacgtactca
     3541 ccctgactgc cacgcccatc ccgcgcacac tgtatatggc attgaccggc gtgcgagata
     3601 tctccacgat taacacccct cctgaagagc gcttgcccat catcacgcat gtggggccat
     3661 accaaccacg gttagtacgc caagccatct tgcgcgagct tgaacggggc gggcaggttt
     3721 tctttgtcca taatcgtgta caaacgatag gtggcatatc ccgccatttg gagcagcttg
     3781 tccccgaagc gcgcattgcc atcgcacacg ggcaaatgcc ggaacacatt ctttcagagc
     3841 gcatgaagca attcgtcaac ggtgacgtgg atgttcttgt gagtacgtca attattgagt
     3901 ctggcctgga tattcccaac gcaaatacac tcatcgttga ccgcgccgat agctttggct
     3961 tggcgcagct ttaccaattg cgcggccggg tgggccgcgg tgctcagcgt gcatattcct
     4021 tctttttccg ccacacacgc cggtcgccca cacccgaggg tcgcgagcgg atggaaacca
     4081 tcgcagaaaa cacccaacta ggcgcgggct attcgattgc catgcgagat ctggagatcc
     4141 gcggcacggg tgatatctta gggacacgcc aacatggaca tattgttgcg gttggctttc
     4201 atctttacac ccgattgctg gccgaggctg tccggcaact gcgaccttcg caaccggctc
     4261 tccttcagaa agggtggcac attccgctgc ctagtccggc gaatgtggat cttcccatgc
     4321 ctgccaatat ccctgccaag tatgtggctg atcggacaat gcgcttgcgc ttgtaccggc
     4381 gcatggctga tctccaaact ctgcaggagg tggattctct ggcggatgaa tttgttgatc
     4441 gttttggccc gcttccggta gcggtgagca acctgttttt ccagcttaag gtgaagttgc
     4501 tggcagaaca agccagccta gcttcaatta gcagcgagaa tggacagctt gtgatgcgct
     4561 ttccagacaa ggaagtgcct gcgtcattac ctaatccggg gccatttgtg cgggttggca
     4621 agaccgcact gtggatgccc tatactaatc taccaaattg gcgtgaaacg cttgtggaag
     4681 tattaaagaa tttggcgaat aaagatgggg tagcgatacg agagtagtgc ttctctccca
     4741 tgtcacctat ccctatcatt tacctcccga gacatgttcc cacctggcat acccctctca
     4801 aaaaggcttc ttgacctgct attgaccatc cctggtttag tgttggtctc gcctgtgctg
     4861 ctactcatcg ccctgatggt gcgtattatt catggcaagc cagtgctctt ccgccagtta
     4921 cgtccgggct accggggtca gccattctat gtgtacaaat tccgcaccat gaccgaagat
     4981 cacgatgaag tgggcaacct gttaccagat acccaccggc tgactgcatt aggacgcttt
     5041 ttgcgcacca ccagtctgga tgagctaccg gagttatttc atgtcttggc aggtaaaatg
     5101 agcctggttg gcccgcgccc cttgctgatg cagtatctgg atctttacac gcccgagcag
     5161 gcgcgccgcc atgatgtgct gcctggcatc accggttggg cacaagtcaa cgggcgcaat
     5221 gctattactt gggaagataa gtttcgtttc gatgtctggt atgtggataa ctggtcgctc
     5281 tggttggaca tcaaaatcct ggcggtgact ttgtggaagg tgctcaagcg cgagggtatc
     5341 agccagcctg gacgcgccac ggccgatgaa tttatgggtt cgaagcggga cgagggttga
     5401 gaatgatgga aaacacggcg ttatatcgct ccctttacct catccgtgcc ttcgaagaga
     5461 cagttttagc gaatttccca cgcggcatat tctttggcac cacacatacc tacctggggc
     5521 aggaggcgaa tgctgtgggc gtgctcagcc acctacaagc gggagacgta gtttttagca
     5581 accatcgctg tcatgggcac tatctagcat atggcggtga cccgcgtggc ctgttcgctg
     5641 agctgatggg taaagccacc ggcgtgtgcg gtgggagggg tggctcgcag cacctgcatt
     5701 ggaagaactt ttactcaaac ggcgtccagg gaggcattgt gccgattgcc acgggcatgg
     5761 ccttggcgga gaagcgtaag gggagcggtg cggtgaccgt ggctttctta ggtgatggca
     5821 cgctgggtga gggtgtggtg tatgaagcgc tgaacatggc ttcgctgtgg caggctccca
     5881 tcttgtttgt gctggagaat aatcgtattg cccagacaac tcctgtcgag ctggcggttg
     5941 ctggggaaat ccctgcccgc ttcaatgcct ttggcatccc ggcacgccag ttagatagca
     6001 gcgatgtgaa tgagattgtt ccgctagcag gtgaattgat gaaagaggta cgtaccttga
     6061 gcgtgccgcg ggcgctgata ctgcatacct accgctttgg tccccactcc aagggagatg
     6121 acacacgcaa tccacaggat gtcgatcgaa tcaagcaggc gcgagatccg ttatctatcc
     6181 atgccccccg ccttggccct gaagagcgtg ctgccattga agccgatatt aagcagttga
     6241 tcgagcaggc atttcaccaa gctatggtcg atccttttcc agtgattagc gatgcataaa
     6301 ccgtgactgt ttgaaacgca gctacccatg aataccgtcc tcgaaaccct taattctgcc
     6361 ctgcatcgtg ccttcaccga tgatgcgcac gtattgctgc ttggtgagga tgtgcttgat
     6421 ccttatggag gggctttcaa ggtgacgcgc ggactttcca cgcgcttccc tgaacgcgtg
     6481 ttaaccaccc ccatctctga ggcgggcatt gttggggttg cggcagggat ggctttacgc
     6541 ggcctgcgtc cggtggtgga gatcatgttt ggcgactttg tcgctttggc tgccgaccag
     6601 ttggttaacc acctcgccaa gttccgctgg atgtacaatg agcaggtgcg cttgcctttg
     6661 gtgatccgca ccccgatggg tgggcggcgc ggctatggac ccacccacag ccaaacgctg
     6721 gagaagcttt acctggggtt gcccggcttg cgggtgatag ctcctacggc actaggaaat
     6781 ccgggcgact tactctacga tatgatactg cacaccggag acccggtatt attcattgaa
     6841 aataagctgc tgtatgccat gccgctccac ggcgaagatt tggtcgagga ttttatccct
     6901 tatcccttat cccctatccc atcagagatt ggtggtgaga ggcagggaat aggggatcat
     6961 tccaatccca cctatgggct ctccttgcgc ggggcgccgc cccctcaatt gaccatcacc
     7021 gcatacggtt acatggctga gctagcctta caagccgtcc gccgcttggc ttaccagcat
     7081 gagatttttg tcgagctgat agtccccaca gagctatcag ctttcgacct cgaaccgctg
     7141 ttctcctccc ttgagcgcac gcacagattg ttaaccgtag aagaaggcac ctacaccttg
     7201 ggatggggag cagaggtcgt tgcacgcgcc gctgagaagc ttggcccgcg cttacatgcc
     7261 tcccgtcgac tggcagccca ggatctgccc atacccgcct ccggtccttt ggaatctgcc
     7321 gtgttgcctg gtgtggatga gatcataagc gtagcgctga aactctccac gggtaaagat
     7381 cggaaacatg acctatcccg gtaatagggg gatgagaacc gaaaaaaagt cctgtttgtg
     7441 ggccgaggcc cacaaacagg actttttttc tatcaatctc cctcccccga gtttggggga
     7501 gggtcggggt gggggttgaa gtatgaaatt aacccccgga aggattgaat ttcgggtgtt
     7561 tcatgcctct tctccctgcc ccttgaggag tggcctaccc aggggtcttg gaagaagctc
     7621 cgcagtaaag tgtagcgtta cttcgagcta aatctttggt aaacgcactg caggttgcca
     7681 gacgatttgt ctttcaggca aatcatcagc tttttgaccc tcagctccga agggagtttg
     7741 aagcggatcg ccttgcgatt gccttgcgtg gcgatattga tcttgccgat atcgaccagt
     7801 ttccatttgc tgtccccggc attctcgcct ttgaccagga cagtggtctt ggggtaattg
     7861 tacgtataga tgatgatatc gttccccgcc cggctggcaa cgaagaaaac cgcgtaggtg
     7921 ttggtgtttt tgctcggcag attggataga cccttcttcg tcctcttggg aatgcacaac
     7981 ctctggccga tatagatagt atagggcttc ttcatatcat tctctgcaac gattgtcgcc
     8041 acagacacgc caaaacgcct tgcaattttt ttgagcgtat cggttcgtgt cacttcgtat
     8101 gaacgcttac aatcgtcctt cgcatccagc ggagccgcta aaacaccttg cggcagcata
     8161 gagaccagca ggctgagcac caacaatatc gccagccagc gagccatcct gggtaactcc
     8221 tttgaatttg cgcctgggtt catgatgcat tctcctttcc tactcaaaaa tcacgcctgt
     8281 agcgaaggca cctgaattca ttatacaata aatttccgct tgtagtggtg gacgcaagta
     8341 cacccgctcc tatgccccct tttttcccct aactctcctc aacttccact ttcaccattt
     8401 gatctccctg ctcgatcgca ttgacgacat catttcctgc gaccaccttt ccaaacaccg
     8461 tgtgtttgct gtccaggtgc ggctgggggg agtaggtgat aaagaactgg ctgccgttgg
     8521 tgttcggtcc agcgttagcc atggagatca cattggtctc gtggcgcagc gggttgccac
     8581 gcgtctcatc ctcaaaacga tatccaggtc cgccgcgccc ggtcccggtt gggtagcctc
     8641 cttgaatgac aaaattggcg atcacccggt ggaaggtcac accatcatag aagccctcgc
     8701 gggctaaaaa gacgaaattg ttggctgtct tgggggcgtg ctgcgggtag agctcaagtt
     8761 tgatcaggcc tttgtttgtc tccatcttga tcacatagct ctttttcgga tcaatctgca
     8821 tttcgggtgc agtattccat tgtttggtcg tcatgagtgg tttctcctga ttagctcgaa
     8881 cggttattgc gagcgttatc tgcgcagcaa tctcgcagtc gcccttttgt gtagcccagt
     8941 cagggctttc atatagtata cgatgaaatg gggggaaatt cccgctcgtt gtggtggacg
     9001 tcagtccacc gcctgtctcg ctcaccttga tatgtgacac cggaacacgt aaacacctcg
     9061 gctcaccc
//
LOCUS       DBNHOH010000960         1909 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_573519, whole genome shotgun sequence.
ACCESSION   DBNHOH010000960 DBNHOH010000000
VERSION     DBNHOH010000960.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_573519"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="AC16V7_17330"
     CDS             complement(<1..360)
                     /locus_tag="AC16V7_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015813707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HIR6258941.1"
                     /translation="MENRDNPVRVRFAPSPTGHTHLGSGRTALYNYLIARQTGGQFLL
                     RIEDTDRKRYVPGAEEELIDSLHWLGLQWDEGPDVGGPYAPYRQSKRKEIYQHHARKL
                     VEMGHAYYCFCTPQRLAQ"
     gene            complement(373..1506)
                     /locus_tag="AC16V7_17335"
     CDS             complement(373..1506)
                     /locus_tag="AC16V7_17335"
                     /inference="COORDINATES: protein motif:HMM:NF013279.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR6258942.1"
                     /translation="MQTSSSSNWRHFDFWLFGAVVVLTIFGITMIRSAVAGNIELTTE
                     TNLVNRQITFTIVGFVAMFLTASIDYHYWSSASRVLYLGTAVALIILNVIAQTVFGSA
                     RWFQVLTINIQPSEIAKIVIILVLADFFTQNYEEVGNLKIIRNSLLYTLGIMIWVLLQ
                     PNLSTSIVIMVIWFSLLWAGGLKLKHLLLFIGAGLLAAIILVPIMIQLDIIKEYQIAR
                     VTSFLFPDPNARFGDIYNIQQALISIGSGGWFGQGYGGGSQVQLRFLKVRWSDYIFSA
                     MAEEFGFVGTLIVLAVLAFVIYRCLRAARLARDTFGALIAYGVSTLIAFQAMVNIGVN
                     LNLLPATGLTLPFVSYGGSSLLSALLGIGLVESVLLRQKALEF"
     gene            complement(1587..1856)
                     /gene="minE"
                     /locus_tag="AC16V7_17340"
     CDS             complement(1587..1856)
                     /gene="minE"
                     /locus_tag="AC16V7_17340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HIR6258943.1"
                     /translation="MSRFFDRLLGRDQKSARQAKERMKLVLIHDRTDLTQSTMEALKD
                     ELIAVISRHVEIEPEAVRIEMAQDGREQRLIADIPLRTVRRRRVG"
BASE COUNT          498 a          497 c          506 g          408 t
ORIGIN      
        1 ctgggcgagg cgttgggggg tacaaaagca gtaataggca tggcccatct cgactaactt
       61 gcgggcatgg tgctggtata tctccttacg tttggactgg cgatatggag catacggacc
      121 acccacgtcg gggccttcgt cccattgcaa tcccagccag tgcaggctat caatgagctc
      181 ctcctcagcg ccgggaacgt atcgcttgcg gtcggtatct tcgatgcgta gcaagaactg
      241 tccaccggtc tggcgggcaa tcagatagtt atacagcgcg gtgcgcccac tacccagatg
      301 ggtgtggccg gttggggagg gcgcaaagcg cacgcgaaca ggattatctc tgttttccat
      361 tagcaaaatc cattaaaatt cgagcgcttt ttgccgcaag agcacgctct cgaccagccc
      421 aatgccgagc aaggcggaga gcaatgaact tccaccgtaa ctcacaaaag gtaaggtcaa
      481 gccggtggct ggcagcaaat ttaaattaac gccgatgttc accatcgcct ggaaggcgat
      541 caaggtggag acaccgtaag cgataagcgc tccaaaagta tcccgcgcca ggcgagcagc
      601 gcgtagacag cggtagatga cgaaagccag caccgccagc acgatcagtg tgccaacgaa
      661 accgaattcc tcagccatgg ccgaaaagat gtaatctgac cagcgcacct tgagaaagcg
      721 taattgcacc tgggaaccac caccgtagcc ctgcccaaac cagccgcccg agccaatgct
      781 gatcagcgcc tgctggatat tgtaaatatc gccgaagcgg gcgtttggat cagggaataa
      841 gaaactggtg acgcgagcaa tttggtattc cttgataatg tctagctgga tcatgatcgg
      901 cacaaggatg attgcggcca gtaggccggc gccgatgaag agcagcaggt gtttcaattt
      961 caaaccacct gcccacaaca aggagaacca gatcaccata attacgatgg atgtgctcaa
     1021 gttgggctgg agtaacaccc agatcatgat ccccagtgta tacagcaaac tgtttctgat
     1081 gatcttgagg ttgccgactt cttcataatt ttgggtgaag aaatcagcca gcaccaggat
     1141 gatgacgatt ttggcgatct cggaaggttg gatattgatg gttagaacct gaaaccagcg
     1201 tgccgagcca aacacggttt gggcgatcac atttaatatg atcagcgcca cagcagtgcc
     1261 cagatacaat actctgctgg cagacgacca gtagtgatag tcgatgctgg cggttaagaa
     1321 catggcgaca aaaccaacta tggtaaaggt gatctgccga ttgaccaggt tggtctcagt
     1381 agtcaactcg atattcccgg ctaccgcaga gcggatcatg gtgatgccga agatggttaa
     1441 gaccaccact gccccaaaga gccagaaatc gaaatgtcgc caatttgaag acgaacttgt
     1501 ttgcatgaac aatccacaac ttgctgagaa aattgctgcc cagttagatc aggtcaatcg
     1561 ggcagatttc tcaagcacac ctgtcattat cccacacgac ggcgccggac tgttcgcaag
     1621 ggaatatcgg caatcaagcg ttgttcacga ccatcctgtg ccatctctat gcgcactgct
     1681 tctggttcta tttcaacatg gcgggagatg acggcgatca gctcatcctt gagggcttcc
     1741 atggtactct gtgtcagatc ggtacgatcg tggatgagaa ccagcttcat gcgctctttc
     1801 gcctgtctgg cactcttttg gtcacgtccg agaagacgat cgaagaaacg gctcatctca
     1861 attgcctccc gggcggaata agcgtgccag ccgccccatt aagttacca
//
LOCUS       DBNHOH010000961         7617 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1023772, whole genome shotgun sequence.
ACCESSION   DBNHOH010000961 DBNHOH010000000
VERSION     DBNHOH010000961.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7617)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7617)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7617
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1023772"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..1225)
                     /locus_tag="AC16V7_17345"
     CDS             complement(176..1225)
                     /locus_tag="AC16V7_17345"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6258944.1"
                     /translation="MASSDFSIRLIAPGDGPALEALLKASPVRGEIGFTYDYQADILA
                     VSQALAVDLVGAVAVQDGAVIGMVYGELLPVQWAGGVCQGVYVSNLRVRPDFQRQGLA
                     RRLGDWGLAYLTERLGRDVVLYAAIPEGNISLNLAQRHGFISTRQIQGGVIPMRRTPP
                     RLIPGLQIRPASREDLPAFAEGVNEFYREHDLWNPVTPASLEIFLERQVRGIRPNQLY
                     IVTRGGEVVGGLSLSDRTQLVRMRVTGASAFVRLLGDLLGVLPRDGVLRALTVREVWF
                     GDGQLEAGRYLWQQLRYRLRERGDCLGIAYDPHDRLADLFQVPFWLPLVKARYLVRAD
                     GSPEGERLIYCIAGA"
     gene            complement(1215..2111)
                     /locus_tag="AC16V7_17350"
     CDS             complement(1215..2111)
                     /locus_tag="AC16V7_17350"
                     /inference="COORDINATES: protein motif:HMM:NF016840.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR6258945.1"
                     /translation="MHEDKALWSDTLVHLMALAAKLEGEGQYNNAKLARAAAESMTRQ
                     AAYVLSLPSERGQLVGEIQKAVSALTSLGADPGLLDAFRLGIDAMAAGRLPLIDETPH
                     PYVCRTCGRVELAQPTKICPTCGAWPATFKQFLPVYWLDALEPFAALERLRQTPIDVA
                     TLLKGMPEELLSREPDDGGWAIRNIVSHLRDAQGVLSFRLNLLLEQDDPVLESKAVFE
                     WAKKEEDRPPTTQEIFDTYNASRQEIVARLEAIPLSHWWRTGRHEEFGTVTLRQQVSY
                     FASHEATHLPQIEFLRDKWVGK"
     gene            complement(2145..2534)
                     /locus_tag="AC16V7_17355"
     CDS             complement(2145..2534)
                     /locus_tag="AC16V7_17355"
                     /inference="COORDINATES: protein motif:HMM:NF019928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HIR6258946.1"
                     /translation="MDKDFIVSDVIHASPEEIYAAWLDSKGHSQMTGSPARASDQVGD
                     TFEAWDGYIQGRNLALEPGKRIVQAWRTTEFGPSDEDSLVEITLKPAASGTRVTIHHS
                     NLPENGDQYRQGWIDFYFQPMKAYFKA"
     gene            complement(2551..3801)
                     /locus_tag="AC16V7_17360"
     CDS             complement(2551..3801)
                     /locus_tag="AC16V7_17360"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR6258947.1"
                     /translation="MTLHIPPSLQHRRFRLLWLGLMISVAGAQMQVWALFWHIRTLTD
                     QPIALGGVGLARILPVIVFSLLGGAVADVANRRRVLFITQGSIGLIALVLAILTLNGN
                     IALWQIYLLTALQAVAVSFDQPARQALVPNLVPARDLPNAFSMMSIAFQTGAIVGPAL
                     SGLIIAYWGQPYTYLINAASYLAVILALILLGPVSQQTAVGRDARINLPAIREGVQFI
                     MSQPIILSSMILDFFATFFSSANTLMPIVARDILHVGAVEYGWLSAAQSVGAVTAALA
                     ISQMSAIRRQGPIFLGAVVVFGAATVVFGMVRGFLLAFFALMVIGAADAVSTIIRNTI
                     RQLHTPDYIRGRMTSVNQIFFMGGPQLGEVEAGVVAQLFGAPFAIISGGLGCILAVTW
                     IARRWPQLRAYNGDEPLRAGAPAD"
     gene            complement(3867..4622)
                     /locus_tag="AC16V7_17365"
     CDS             complement(3867..4622)
                     /locus_tag="AC16V7_17365"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6258948.1"
                     /translation="MSEFKGKVVLVTGAGRGIGRALAMAFAAQGARVAANDLTPVNLD
                     ETLKRITAEGGECKDFVFDVAGKMPVQAMIEEIRDEWGRIDILVNNAGVEPHASLLEM
                     DEWDWRRTLDVNLNGPFFTMQSVGRVMREQGGGIMVNIASIAGRAHGLKDRSAYVASK
                     MGLIGLTREAARELAAYNIRVNAICPGVIETEMTAALRQDEAMLRKWLEDIPQGRLGR
                     VDDVVGLTLFLCSEAAAYVTGQAINVDGGKVMC"
     gene            complement(4655..4993)
                     /locus_tag="AC16V7_17370"
     CDS             complement(4655..4993)
                     /locus_tag="AC16V7_17370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HIR6258949.1"
                     /translation="MNKLVILIEPPDDWEVFDNTWPQFLHHAENMPGLRREATSRVDT
                     FLYGERTYALMHELFFDSLDAAQQAMASPEGRQAGRILQEMSRGRMVLFFADHKEDDL
                     ENIKKMKGDR"
     gene            5140..5523
                     /locus_tag="AC16V7_17375"
     CDS             5140..5523
                     /locus_tag="AC16V7_17375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258950.1"
                     /translation="MDEKITIIEGPPPTFETVADGWVLGLNEGPKLGNIAVTRLRTFN
                     GPALVERCYRAWHNQQTILLEYRSSDGLTQTTPIVAARHMELEDGQLLELWVRLTNDE
                     VQLELGYEDDLGDDMDDDLDNEDLL"
     gene            5648..6424
                     /locus_tag="AC16V7_17380"
     CDS             5648..6424
                     /locus_tag="AC16V7_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6258951.1"
                     /translation="MSEYVLTEQKNNLLVITLNRPKVNAFNLEMIKALQDALKQARRD
                     AQVRCVLLTGSGKVFSAGQDVTEVKEAEDTSFRYHLMRTYNPLILQIRQLEKPVLAAL
                     NGAVSGAAMGIALACDLRIAADTARFVVGFLGIGLGPDSAVSLLLPATIGLGRATEYA
                     FTNAPINAEEALALGLVNRVVPAKELIQKSFELASSFAQGPVGSFGLTKRAFNKAVLS
                     NLEQVLDYEAHIQDIAGRSDEHKEGVEAFLEKRSPNYVNS"
     gene            6906..7127
                     /locus_tag="AC16V7_17385"
     CDS             6906..7127
                     /locus_tag="AC16V7_17385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258952.1"
                     /translation="MLERRYQSDLNYIKAHHRELIQAAQQEKLLGQTKASDPNLKERL
                     LDAAGNRLIAWGQKLKGETYSNELSQECV"
     gene            7141..>7617
                     /locus_tag="AC16V7_17390"
     CDS             7141..>7617
                     /locus_tag="AC16V7_17390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258953.1"
                     /translation="MSDRDKTPKGQPRLGWDSGSAYDFFFSLEVLHNPDQYGLRASWA
                     AGVRSRLPTQERKLLEEIQPYLGVPLNWIYRLPAPKDATSAIWALRQTPPAERMVNLA
                     GYCDMEDLAVIYKQVAERRSWDNNDVKVIHEILNQHKKEKRYVSRFLKDLPKCLDWW"
BASE COUNT         1842 a         2152 c         1975 g         1648 t
ORIGIN      
        1 aaccgcctcc ggcctgatgg ggggaggaaa aaggaatgat gatggcggga cagctttcca
       61 aacgcgttca tattgggcgg cagtttacca taaagaaaat aaagagtcaa ataggatttt
      121 tcgaatctac cctctatccc tcagcagctt gcgaatctgg catatatgat taggattaag
      181 ccccagcaat acagtaaatc aggcgttcgc cctcgggcga accgtccgcc cgcaccaggt
      241 agcgcgcctt caccagcggg agccagaaag gcacctggaa taaatccgcc agccgatcat
      301 gcgggtcgta ggcaataccc aaacaatccc ctcgctccct caaccggtag cgcaactgct
      361 gccagaggta gcgcccggcc tccagttgac catccccaaa ccagacctca cgcacggtca
      421 gggcgcgcag caccccatct cttggtagca cgcccaacaa atcgcccagc aaccgcacaa
      481 aggcggaggc acccgtgacc cgcatgcgca ccagctgagt gcgatccgac aggctcaggc
      541 cgcccacgac ttctccgccg cgcgtcacga tatagagctg gttcggtcgg atacccctca
      601 cctgccgctc tagaaatatt tctaaggagg cgggcgtcac aggattccac aggtcgtgtt
      661 ctctataaaa ctcgtttacc ccctcggcga aagcgggcag gtcttctctg ctggcaggtc
      721 ggatttgcaa gcctgggatc agtcggggcg gagtacggcg catggggatc acgcctcctt
      781 ggatctgtcg cgtggagata aatccgtgac gctgtgccag gttgaggctg atattgccct
      841 cggggatggc cgcgtacaag actacatccc gcccaaggcg ctccgtaagg taagccaacc
      901 cccaatcccc caaccggcgc gccaaaccct gccgctgaaa atctggtcgt acccgcaaat
      961 tggatacgta cacgccctgg cacaccccac cggcccactg cacaggcagc agctcgccat
     1021 acaccatccc tatcaccgca ccatcctgaa cagccaccgc accgaccaga tcaacggcca
     1081 gtgcctggct gaccgccagg atgtccgcct gatagtcata ggtgaagccg atctccccgc
     1141 gcaccgggct ggctttcagc agtgcttcca gcgccggacc atcccccggt gcgatcaggc
     1201 ggatgctgaa gtcgctactt gccaacccac ttatcccgca aaaactcgat ttgaggcagg
     1261 tgggtggctt cgtgagaggc gaagtaactc acctgctggc gaagcgtgac cgtgccaaac
     1321 tcctcgtgcc ggcctgtgcg ccaccaatga ctcagaggga tggcctccag cctggcgacg
     1381 atctcctgtc gggaggcatt gtaggtatcg aaaatttctt gcgtagtagg aggacggtct
     1441 tcttctttct tcgcccactc gaagaccgcc tttgattcca gcacggggtc atcctgctcg
     1501 agcagcaaat tcaagcggaa gctcagcacc ccctgagcat cccttaaatg ggacacgata
     1561 ttccggatag cccaaccacc gtcgtctggc tcccggctga ggagctcctc tggcatccct
     1621 ttcagaagcg tggcgacgtc gatgggcgtc tggcgcagcc tctccagcgc agcaaagggc
     1681 tccagggcgt ccagccagta gaccggcaag aattgtttga aggttgcagg ccacgccccg
     1741 caagtcgggc agattttggt tggttgagca agctcgacgc gcccgcaggt gcgacaaaca
     1801 tacgggtggg gcgtctcatc gatcaggggc agtcgcccgg cagccatggc gtctatgcca
     1861 aggcggaagg catccagcaa gcccgggtct gcccccagcg aggtgagcgc cgaaaccgct
     1921 ttttggattt caccaaccag ctggcctctc tcagacggca ggctaagtac gtacgccgcc
     1981 tggcgcgtca tggactcggc tgccgcgcgc gctagcttgg cgttgttata ttgcccttca
     2041 ccctccagct tggctgccag ggccatcaaa tggaccaaag tatcactcca caacgcttta
     2101 tcctcatgca taaaaccacc tccagataat cactatttgg cttctcacgc tttgaaataa
     2161 gccttcatcg gctgaaagta gaaatcgatc cacccttgcc ggtactgatc gccgttctct
     2221 ggcagattgg aatggtgtat tgtcactctc gtcccactgg ctgctggctt taaagtgatc
     2281 tcgacaagcg aatcttcgtc agaaggtcca aactccgtgg tccgccaggc ttgcacaatg
     2341 cgcttgcccg gctccaaggc aaggtttcta ccctggatgt acccgtccca ggcttcaaac
     2401 gtatcgccca cctggtcgct cgccctggca gggctgcccg tcatctgcga gtgacctttg
     2461 gaatccagcc atgcagcata tatctcttct ggtgatgcat ggatcacatc tgatacgata
     2521 aaatctttgt ccatatccta ctccatagcg ctaatctgct ggagcacccg ccctgagcgg
     2581 ctcatctcca ttatatgcgc gcagctgagg ccagcggcgg gcaatccagg tcaccgctaa
     2641 gatgcacccc aacccgccgc tgatgatggc gaacggcgcg ccaaatagct gcgcaacaac
     2701 acctgcttcc acctctccca gctgcggacc gcccatgaaa aatatctggt tcacgctggt
     2761 catgcgcccg cggatataat ctggcgtgtg caactggcgg atggtgttgc ggataatggt
     2821 actcaccgcg tccgccgcac caatgaccat cagggcgaaa aaagcgagca gaaaaccacg
     2881 caccatccca aagacgaccg ttgccgcacc gaataccacc accgccccca aaaagatcgg
     2941 cccctggcgg cggatggcgc tcatttgcga aatagccagc gccgccgtca ctgcccccac
     3001 cgattgcgca gctgagagcc atccatattc cacagcacct acatgaagga tatcgcgcgc
     3061 cacaatgggc atcaaagtgt tggctgagga gaaaaaggtg gcgaaaaagt ccaatatcat
     3121 actggagagg atgatgggtt ggctcattat gaactgcacg ccctctcgaa tcgccggcag
     3181 gttgatcctg gcatcccgac cgacagcggt ctgctgcgag acgggtccca gtaagatcag
     3241 cgccaggatc acagccaggt aagaggccgc gttgatcagg taagtatatg gttgtcccca
     3301 gtatgctatt atcagcccgc tcaacgccgg tccgacaata gctccggtct gaaaggcgat
     3361 ggacatcatg ctgaacgcgt tgggtagatc acgagcgggt accaaattcg gcaccagtgc
     3421 ctggcgcgcc ggctggtcaa aggatactgc cactgcctga agcgcggtta acaagtagat
     3481 ctgccacaaa gcgatgtttc cgttgagggt caaaatagcc aggacaaggg cgatcagccc
     3541 aatggaaccc tgggtgatga aaagcaccct gcgccggttt gcgacgtcgg cgacggcccc
     3601 acccaaaagc gagaatacaa tcaccggcag gatacgcgcc aatcccactc cacccagggc
     3661 tatcggttgg tcggtgagag tgcgaatgtg ccagaaaagc gcccatacct gcatctgggc
     3721 accggcaaca gagatcatta accccagcca gagcaaacgg aatctgcggt gctgcaaaga
     3781 aggagggata tgtaacgtca tagggctatt agttatcggt gtagggaata tttattatgc
     3841 aatgcacata tccggaatgc gagactttaa cacatcactt tcccaccatc cacattaatc
     3901 gcctgcccag ttacataggc agctgcctca ctgcacagaa acagcgtcag accgaccaca
     3961 tcatcgaccc gtcccaagcg accctggggg atgtcttcca accatttgcg cagcatggcc
     4021 tcgtcctggc gtaatgcagc cgtcatctct gtctcaatca cccccggaca gatagcgttc
     4081 acacggatat tgtaggcggc gagctcgcgg gcagcttcac gagtcagccc aatcaaaccc
     4141 atcttgctgg ccacataggc ggagcgatcc ttcaaaccgt gtgctcgacc ggcgatagag
     4201 gcgatattga ccataattcc tccaccttgc tcacgcatca ctcgcccaac cgattgcatg
     4261 gtgaaaaatg gaccgttcag gttcacatcc agtgtgcggc gccaatccca ctcatccatc
     4321 tccagaaggg aggcgtgcgg ttctacaccc gcgttgttaa ccaggatgtc aatgcgcccc
     4381 cactcatcgc gaatctcctc gatcatcgcc tgcacaggca tcttgccagc cacatcgaag
     4441 acaaaatctt tgcattcccc accctcagca gtgatgcgct tcaacgtctc atccagattc
     4501 accggggtga gatcgttagc agccacccgg gcgccctgcg cggcgaatgc cattgccagc
     4561 gctcgcccaa tacccctccc ggcgccagtg accaggacaa ctttcccttt gaattcgctc
     4621 atatcacctc aattgaaaac agcgtctcgt gccttcaccg atcacccttc atcttcttga
     4681 tgttttccag gtcatcttcc ttgtgatcgg cgaaaaacag caccatgcgc ccgcgcgaca
     4741 tctcttgcaa gattctgcca gcctggcgtc cttcagggga agccatggcc tgctgggctg
     4801 catccaaaga gtcgaagaac aactcgtgca tcaaagcata ggtgcgctct ccatacaaga
     4861 atgtatccac gcggctggtc gcctcgcgtc gcagaccagg catattctcc gcatggtgca
     4921 ggaattgagg ccaggtgtta tcaaatacct cccaatcatc aggaggctca atcaatatta
     4981 ctaatttgtt catcttgacg tctccgcctt gattatatac acaagctacg cagttggcga
     5041 gggaaagaag tgatccctca cacatctcta ttttgtacaa ttgcgtaagc attattaaaa
     5101 ctaaattgtg gtatgattcg acagattaga ggcaaatcta tggatgaaaa aatcaccatc
     5161 attgagggcc ctccgccgac atttgaaact gttgccgatg gatgggtact cggtttgaat
     5221 gaaggtccaa agctgggcaa tatcgctgtc acccgcctgc gcacgttcaa tggcccagcc
     5281 ctggtggaac gttgttatcg cgcctggcac aaccagcaga ccattctgct agaatatcgc
     5341 tcctcagatg ggttgacgca gactaccccg attgtcgccg cccgacacat ggagctcgaa
     5401 gatggccagt tgctggaact atgggttcgc ctgaccaacg atgaggttca attagagctg
     5461 gggtacgagg acgatctggg ggatgatatg gatgacgacc tggataacga agacttgctt
     5521 taaaccagta cagattaatc acataaattg agggatgcgg gaacaaccac ccacatccct
     5581 caattttttc tttcaggttg ggataaattg tgctaaactc gtgtcaatcg cctggattgg
     5641 aggtactatg tctgaatatg tgctaaccga acagaaaaac aacctattgg tgataacgct
     5701 caaccggcca aaggttaacg ccttcaatct ggagatgatt aaggccctcc aagatgccct
     5761 caagcaggcc cgccgcgacg cccaggtgcg atgtgttttg ctaaccggca gcggaaaggt
     5821 attcagcgcc gggcaggatg tgaccgaggt gaaggaagcc gaggacacat ccttccgtta
     5881 tcacctaatg cgaacctaca atccgttgat cctgcaaata cgccaattgg agaaacctgt
     5941 cctggcagct ctcaatggag ctgtttccgg tgccgctatg gggatcgctc tggcatgcga
     6001 ccttcgcatc gcggctgata ctgctcgctt cgtggttggg tttctgggta tcggcttggg
     6061 ccctgactcg gcagtctcat tgttgctgcc tgcgacgatt ggcttggggc gagcaacgga
     6121 atacgccttc accaatgcgc ccataaatgc agaagaggcc ttagcgctag gacttgttaa
     6181 ccgcgtggtg ccagctaaag aactcatcca aaagtccttc gaattggctt catcttttgc
     6241 tcaaggtcct gtgggttcct ttggtctgac caaaagagca ttcaataagg ctgtgttgag
     6301 caacctggag caggtgctgg actatgaagc tcatatacaa gatatcgccg gacgaagtga
     6361 tgaacataaa gaaggtgttg aagcttttct ggaaaaacga tctccgaact atgtcaatag
     6421 ttaatcatta tcattatgta ttatttatat atcgtagtct tagtaggctg ggtggataag
     6481 tggataaagt aataacctaa gtgctttact ggcatatatg ggggtgatgg ctcagaggtc
     6541 ggggagatat agtacaagac aaacggcatt gtatccacag ggtggatggg gatagacgcg
     6601 aagaagattt tttgcaggat acccttttgg gagaggggat gatatttagg gtgcaattct
     6661 gttgggaccc catatatggg gctttgtcaa atttcaaaat tcgcacaaaa gatcgagttt
     6721 tccccattaa gtgcgtttta tccacagttt ttcacgagtt atccacaggt tgagcctcta
     6781 aaaagtgccg ctccggaaaa tttgtgtctt gacaaaacga tggagttaga ctattatcta
     6841 attagataaa tatctacgtt agttatatgt ctatattaga tagatgtcta ttgaaggaga
     6901 acgccatgtt agagcgccgg tatcagagtg atttgaatta catcaaggct catcatcgtg
     6961 agctcattca ggctgcacag caggaaaaac tgctagggca aacaaaggcc agcgatccca
     7021 atttgaagga gcgccttctc gacgcggctg gcaaccgcct gattgcctgg ggtcaaaagc
     7081 tgaagggtga gacttattcc aatgaattat ctcaagaatg tgtatagggg gtagcattat
     7141 atgagtgatc gggacaaaac accgaaaggt cagcctcggc tgggatggga tagcggctcg
     7201 gcttacgatt tctttttcag cctggaagtg ctgcacaacc cggatcagta tggtctgcgc
     7261 gcctcctggg cagccggagt cagatcgcgt ctacccaccc aagagcgtaa gctcttggaa
     7321 gaaattcagc cctacttggg ggtgcccctt aactggattt atcggttgcc ggcacccaaa
     7381 gatgccacca gtgcgatctg ggctttgcgg caaacaccgc cagcggagcg catggtgaat
     7441 ctcgcgggtt attgtgatat ggaggatttg gccgtaattt ataaacaggt cgctgaacgc
     7501 cgttcttggg acaataacga tgtcaaggtg atccatgaaa ttctaaatca gcataaaaaa
     7561 gagaagcggt atgtttctcg ctttctcaag gatctcccca agtgcctgga ttggtgg
//
LOCUS       DBNHOH010000962         2802 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1664126, whole genome shotgun sequence.
ACCESSION   DBNHOH010000962 DBNHOH010000000
VERSION     DBNHOH010000962.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1664126"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1226)
                     /locus_tag="AC16V7_17395"
     CDS             complement(159..1226)
                     /locus_tag="AC16V7_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIR6258954.1"
                     /translation="MKKLPVLLTIIMVLSLLLTACGGQATPTEEVAPEAATSEPAETE
                     GAAPAAEDKWCSGTNIIFFPGGAPGGPFETVVYNGAVAAAADLGPNVEYVWSDWNPEK
                     MITQFSEAMATNPDGIAVMGHPGDEAFGPLVDEAEAKGIVVTSMNTQLAALQAKYSSS
                     GFGYVGAILYDAGWALGKESITRAGLQSGDKAFVWGLLAQAGRGERTKGIVEALEDSG
                     IEVIYQEIDNATNADAAAGIPVFVGIMSSNPDIKLVVTDHGNLTGTQQSFFESAGLGP
                     DDVFGSGFDLSAATVEAIRGGWTDLVIDQQQWLQGYMAILQICITHNYAFTGLHIDTG
                     AGFAHKDNVELLAGLVDQQIR"
     gene            complement(1512..2153)
                     /locus_tag="AC16V7_17400"
     CDS             complement(1512..2153)
                     /locus_tag="AC16V7_17400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HIR6258955.1"
                     /translation="MLEQLKQALWEYHMELPRNNLVVWTSGNVSALDRESGYVVIKSS
                     GVRYEALRPEHMIVVDMDGKVIEGNLKPSSDTASHLYIYRHRSDVGGIVHTHSRYATA
                     FAAVGKTIPVYLTAHADEFGCEIPCAGFALIGGEEIGMQVVKNIGKSTAVLLKNHGVF
                     TIGKDAESAVKSAVMVEDIAATVWIALQLGTPDRISSEDVEKLHHRYTHVYGQ"
     gene            complement(2161..>2802)
                     /locus_tag="AC16V7_17405"
     CDS             complement(2161..>2802)
                     /locus_tag="AC16V7_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HIR6258956.1"
                     /translation="CVPASYEREAKERGVDIHALLEEKAAQLKPGESGLLALDWWNGN
                     RSVLVDVDLTGLLIGATLSTKPEEIYRALIEATAFGTRVIVETFQQNGVPIYELVACG
                     GLPEKNKLLMQIYADVTGRPFKVSSSQQTSALGSAMFGAVAAGSAAGGYDSIYEAAQK
                     MAKLKDEVFIPILEHQKVYDQLYAEYLRLHDYFGRGENTVMKKLKRIKVEARM"
BASE COUNT          679 a          757 c          655 g          711 t
ORIGIN      
        1 ctaggcgata ttgctccata ttcctctcca aaagtgctca atcacgaata cattgattgt
       61 aacattcatc aaggtgacag tcacctcgga agtgactgtc acctgattgc tcatccggtc
      121 agatagaccg aaattggaat acggaatcgg atttaacctt agcgaatttg ttgatcgacc
      181 aaccccgcca acagctcgac attgtcttta tgggcaaagc ccgcgccggt gtcgatatgc
      241 agcccggtga atgcgtagtt gtgggtgatg cagatctgca agattgccat atatccctgg
      301 agccattgtt gctgatcgat caccaggtct gtccagccgc cgcggatagc ttctacagtg
      361 gcggcggaga ggtcgaaccc agatccgaag acatcatccg ggcccaagcc agcactttca
      421 aagaaggact gctgtgttcc ggtcaagtta ccgtggtccg tgacaaccag tttaatatcc
      481 gggttggagg acatgattcc cacgaatacc ggtataccag cagcagcatc cgcgttagtg
      541 gcattgtcga tctcctgata gattacctct ataccagaat cttcgagagc ctcgacaatc
      601 cccttggtgc gctcgccgcg tcccgcctgc gccagcaacc cccagacgaa agccttatca
      661 ccggattgca atccagcgcg agttattgac tctttgccta aggcccaacc ggcatcgtaa
      721 aggatcgcgc cgacatagcc aaagccacta gaggaatact ttgcttgcaa ggccgcaagc
      781 tgagtattca tgctggtcac gacaatgccc ttagcctcag cctcatccac aagagggccg
      841 aaagcttcat cgccagggtg acccatgacc gcaatcccat cgggattcgt agccatggcc
      901 tccgagaatt gggtgatcat cttttcggga ttccaatcgg accacacata ttccacgttt
      961 ggacctaggt cagccgcggc ggcaaccgcg ccattataga ccacagtctc aaagggacca
     1021 ccgggagcac cacccgggaa aaagataatg ttggtgcccg agcaccattt gtcctcagca
     1081 gcgggtgcag cgccttcggt ctcagcgggt tcgcttgtcg cggcctcagg tgcaacttct
     1141 tcggtggggg tcgcttgccc gccacaggca gtcagcaaca agctgaggac cataataata
     1201 gtgagcaaca ctggaagctt tttcatcttt tcatctcctt atactcatct ggtattgata
     1261 gaatggaaac aaaatgcggg gagtctttta cttacccatg gacacctcct cttgcaggtg
     1321 tggtctggca tcaagtggat cattaagata ggtgcatgat tggtcaaatt catgtgatct
     1381 tgttatcgtt cccgttatcg ttcacaaaat cgtaactata ttatcaataa aaaaaggggg
     1441 ggtgtcaaga ggttgcgaat ttgtgctgat ccatatcaaa cccggttcat gtatgatcgt
     1501 gcgatagaaa tttactgccc atatacgtgg gtatagcggt ggtgtagttt ctccacatct
     1561 tcagatgaga tcctatctgg tgtgccaagc tggagcgcta tccagacggt ggcagcaata
     1621 tcctcgacca tcactgccga cttgacggca gattctgcat cctttccaat tgtgaagacg
     1681 ccatggttct ttaatagaac agccgtcgat tttccaatat ttttgactac ttgcatgcca
     1741 atctcttcac ctccaatcaa cgcgaatcca gcacaaggga tttcacagcc aaattcatct
     1801 gcgtgtgctg ttagataaac agggatagtt ttgcctaccg ctgcaaatgc ggtggcatat
     1861 cgcgagtgtg tgtgtacaat accgccaaca tcagagcgat gacgatagat atacaaatgg
     1921 cttgccgtat cagaagatgg ctttaggttc ccttcgatca ctttcccatc catgtcaacc
     1981 acgatcatat gctcggggcg caatgcttca tatcgtaccc cagatgactt gatcactaca
     2041 taacctgatt ctcggtccag tgcactgaca ttaccgctcg tccagaccac cagattgttc
     2101 ctgggcaatt ccatatggta ttcccaaaga gcttgtttca gttgttctaa cataattcac
     2161 tcacattctt gcttcaacct tgatacgctt aagcttcttc atcacagtat tttcaccacg
     2221 cccaaaatag tcatgcaaac gtaaatactc tgcatacaac tggtcataga ctttctggtg
     2281 ttcaagaatg ggtataaaca cttcgtcttt tagcttggcc atcttttgcg cggcctcata
     2341 gatcgagtcg taaccacccg ccgcgctacc tgccgctacc gcgccgaaca tggcggaacc
     2401 tagagcagat gtttgttgag atgaactaac cttgaatggt cgtcctgtca catcggcgta
     2461 aatttgcatc aggagtttgt tcttttctgg caaaccacca catgccacca actcgtagat
     2521 gggtactcca ttttgctgga atgtctccac aattacgcgt gtgccaaagg cagtggcttc
     2581 gatcagagcc cggtagatct cttcaggttt tgtgctcagg gtagcaccga tcaggagacc
     2641 ggtcaggtcc acatccacca gcacagagcg attgccgttc caccaatcca gcgccagcag
     2701 gccgctttcc cctggcttga gttgggcggc tttctcttcg agcaaggcgt ggatatccac
     2761 accgcgttct tttgcctcgc gctcatagga tgccgggacg ca
//
LOCUS       DBNHOH010000963         1965 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_638875, whole genome shotgun sequence.
ACCESSION   DBNHOH010000963 DBNHOH010000000
VERSION     DBNHOH010000963.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_638875"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="AC16V7_17410"
     CDS             complement(<1..367)
                     /locus_tag="AC16V7_17410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048476 - Holliday junction resolvase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HIR6258957.1"
                     /translation="MLVIGVDPGTAITGYGLVRENADGSLISVDYGVIQTSSDLDMPQ
                     RLLELHRVLNEILLLHRPESGAVEKLFFQRNVRTALSVGQARGVALLALAEAEVPIAE
                     YTPLEVKQAVAGYGGADKNQ"
     gene            complement(387..1142)
                     /locus_tag="AC16V7_17415"
     CDS             complement(387..1142)
                     /locus_tag="AC16V7_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014027655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR6258958.1"
                     /translation="MSGHSHWATIRRKKGAADAKRGQIFTRLAREIVIAAREGGGDPS
                     TNIRLQYAIDRARAQNMPKENIERAIKRGTGESKEGNAIEEVTYEGYGPHGIALMISC
                     VTDNRNRAVAEIRHILNRMGGTMAEAGSVAWQFTRKSYFSFPSEGHDTNTIFDLVVEA
                     GADDVTFEDETVEIFGPVELFKEISDHLKSANIQPEEAELRMFPNNEVELSPDESMQV
                     YKVIDALEELDDVADVFSTLSISDEVMAQLEAA"
     gene            complement(1223..>1965)
                     /locus_tag="AC16V7_17420"
     CDS             complement(1223..>1965)
                     /locus_tag="AC16V7_17420"
                     /inference="COORDINATES: protein motif:HMM:NF019845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR6258959.1"
                     /translation="GVPWLFIACRSCEFCARGQENLCPNARFTGFHVDGGYAESMLAE
                     AAYALPLPAGVTAQQAAPLLCAGIVGYRSLRLSDVQPGETLGLIGFGASAHLVIQVAL
                     HWGCTVYVFTRSYEHRRHALELGAAWAGGIEDQAPRMLDRAVLFAPAGYLVPLILEKL
                     RPGGTLAINAIHTSPIPEMPYHLIYGERTLRSVANATYKDGVEFLQLAAEIPVRSTVT
                     AYSLDEANRALSDMKHSRINGEAVLIIS"
BASE COUNT          442 a          593 c          500 g          430 t
ORIGIN      
        1 cctggttctt atccgcgccg ccgtatccag ccacggcttg cttaacctcc aggggagtgt
       61 attccgcaat tggcacttca gcctctgcca gcgccaggag cgccacaccg cgcgcttgcc
      121 ccacactaag ggcagtacgc acattacgct ggaaaaagag cttctccacc gcgccgctct
      181 caggccgatg gagaagtaag atctcattta aaacccggtg tagctccaac aaacgctgtg
      241 gcatgtccaa atctgaggag gtttgaatca cgccataatc cacggatatc aagctgccat
      301 cggcgttttc ccgcaccaat ccatatccgg tgatcgctgt tccagggtct acgccaataa
      361 ccagcatgct cactggcaat ccaccgttag gcggcctcca attgggccat cacctcgtcg
      421 gagatgctta aggttgagaa gacatccgcc acgtcatcca gctcttccaa tgcatcaatc
      481 actttataga cctgcatcga ttcgtctggg ctcagctcaa cctcattatt ggggaacatt
      541 ctcaattccg cctcttctgg ctggatgttg gccgatttca aatgatcgct aatctcttta
      601 aatagctcta ccggtccaaa aatttcaacg gtttcgtctt caaaggtcac atcatcggcg
      661 ccggcttcaa ctaccagatc gaagatcgta ttcgtgtcat gcccctcgga cggaaaagag
      721 aaatacgact tgcgagtgaa ctgccaggcg accgagccag cctctgccat cgtgccgccc
      781 atgcggttaa ggatatggcg aatttcagct accgcccggt ttcgattatc ggtaacgcaa
      841 gagatcatca gggcaatccc atgcggacca tacccttcat acgtcacttc ctcaattgcg
      901 ttgccttctt ttgattctcc cgtgccgcgc tttatggcac gttcgatgtt ctccttgggc
      961 atgttttgcg ctcgggcacg gtcgatagcg tactgcaaac gtatattcgt gctagggtcg
     1021 ccgccgccct cacgcgccgc gatcacgatc tcgcgtgcca gccgggtaaa aatctggcct
     1081 cgtttggcgt ctgcagcacc cttcttgcgc cggatagttg cccaatggga atgtccagac
     1141 atataaacct ctcctgcaaa tgaaataacg caaattatca acttggggat cgtacactac
     1201 cctgtagatt ataccattcc gatcaactta tgatcaacac cgcctctccg tttatgcggc
     1261 tgtgcttcat gtctgagaga gctcggttgg cctcgtccag cgagtacgcg gtgaccgtgg
     1321 agcgaacagg gatttcagcg gctaattgca gaaattccac gccatccttg tacgtggcgt
     1381 tggcgacact ccgcagggtg cgctccccat agatcaaatg gtagggcatc tctgggatgg
     1441 gtgaggtgtg gatggcgtta atcgccagcg tccctcctgg acgcagcttt tctagtataa
     1501 gaggcacgag atacccagcc ggcgcaaaga gcacggcgcg atccaacata cgaggagctt
     1561 gatcttcaat gccacctgcc caggctgccc caagctccag ggcgtgccgg cggtgttcgt
     1621 acgagcgcgt gaagacgtac accgtgcagc cccagtggag cgccacctgg ataaccagat
     1681 gcgcgctggc tccaaatcct atcaggccaa gggtctcacc gggctgcaca tcggaaaggc
     1741 gcagcgagcg gtatccaaca atgcctgcgc acaacaacgg cgcagcctgc tgcgccgtga
     1801 cccctgcggg cagtggcaga gcataagctg cctcggctag catgctctcc gcgtaacccc
     1861 catcgacatg aaaaccggta aaacgagcat tggggcataa gttttcctgc ccgcgggcgc
     1921 agaactcgca ggagcgacaa gcaatgaaga gccagggcac accta
//
LOCUS       DBNHOH010000964         6507 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1023882, whole genome shotgun sequence.
ACCESSION   DBNHOH010000964 DBNHOH010000000
VERSION     DBNHOH010000964.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6507)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6507
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1023882"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..414
                     /locus_tag="AC16V7_17425"
     CDS             37..414
                     /locus_tag="AC16V7_17425"
                     /inference="COORDINATES: protein motif:HMM:NF013384.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HIR6258960.1"
                     /translation="MTEPSGTMANQVALPVLTHPGDDVIVSRESHAVWHETGASAANS
                     GVQFTAIGAKGAFTLDEFLEAIKPRGHVIYPPTTLVEIDNTHNRAGGVIFPDSVCTHP
                     THHSGSDTPFQPTWLSYAAEPKR"
     gene            434..1471
                     /locus_tag="AC16V7_17430"
     CDS             434..1471
                     /locus_tag="AC16V7_17430"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HIR6258961.1"
                     /translation="MNSHLNRIHHFKGNPREIGLAAGRLLGDKLAQNINHYIGSLEHS
                     KDMEELHTGALPWLRRLPERFQEEYEGLAEGANIPLQRLAEWGYLEACKSIQCSGTVY
                     LFENQAWVARNNDFYLPELWGYVTVREVDGRIPTINFPMEGDVFTPTGVNKEKLWLHY
                     NYLPVWDNPSPNKPHVPAYVFLTEALEICHTIRDVETFLNEMDRDGGMLLFAVDGKNN
                     EFALFDCMCSKHYRREPSDGWIVGTNHYCAVEDLTLGDDEDSANTINRFRRMESLMQD
                     LATSKTPPSLPTDLIKILADDEIERRDVEVITVYSNVACPSTGEIWFTFGGHPAASQG
                     NWQKIDWPWKD"
     gene            1552..2214
                     /locus_tag="AC16V7_17435"
     CDS             1552..2214
                     /locus_tag="AC16V7_17435"
                     /inference="COORDINATES: protein motif:HMM:NF039623.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5995 family protein"
                     /protein_id="HIR6258962.1"
                     /translation="MQSLIHQWEETSNPQAVFLSCYKMMTSNMLTSIERQEFMDPPWV
                     TNLLHRFADHYFVALESYEKEPTSAPRVWQLAHDTTRDPQVLPLQNLLLGINAHINYD
                     LVITLVDLLSPEWNDHSDEQRASRYYDFCHVNDVIVRTIDVVQDQVLEPAMPGMDILD
                     KLLGSLDELLVSRLITSWREKVWHNVLSLLEATAQDEQWSLLQQIEEEAVGLGKLISL
                     RD"
     gene            2481..2843
                     /locus_tag="AC16V7_17440"
     CDS             2481..2843
                     /locus_tag="AC16V7_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011140688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR6258963.1"
                     /translation="MTITSPLPEDNLPMTPAMFNILLALADGEKHGYAIMLEVEANTE
                     GAVKMGPGTLYGSIKRMLASGLIEECDERPDPALDDERRRYYRLSGLGQRALRLEAER
                     LEGQVRLARLKQVLKGSA"
     gene            2855..3730
                     /locus_tag="AC16V7_17445"
     CDS             2855..3730
                     /locus_tag="AC16V7_17445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258964.1"
                     /translation="MEKVSSASRSLILSIRLYQALLTVYPSEFRRAYGGPMLQVFRDC
                     SQRALHEGGTAGLLALWSRTMLDTVQTALEEHMQRGVDMSKEKFFKLSGWALMLGGLA
                     TMLGWLASTRPDYNRYNARSLPIDQYANAIEFPLIVMGMLLLSVGLIGLFLRYGQGAG
                     GFGRFSLGLGALSGAISAIGAIGLGIFDSEPWWSTFFIGMVVQYLGLTLFGVANLRQR
                     ALPRWNGLPILAGIWIPLSVVVGLIIEQGTGQWVEWPEAVFLIVWLLSMAGLAGLGYL
                     MQSDPQPADPAAAMA"
     gene            4044..5912
                     /gene="typA"
                     /locus_tag="AC16V7_17450"
     CDS             4044..5912
                     /gene="typA"
                     /locus_tag="AC16V7_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HIR6258965.1"
                     /translation="MLIERTDLRNITIIAHVDHGKTTLVDGLLRQARVFRENQHVVER
                     VMDSNDLERERGITILAKNTAIEVWDNLRKQTVKINIVDTPGHADFGGEVERVMNMVD
                     GVLLLVDAAEGPMPQTRFVLKKALEMGHRAIVVINKVDRKDAIPEAVLNQTFDLFIEL
                     GATDEQADFPVIYANAVTAQSGLTPALSPDLQHLFHAILRHIPAPRVDPDAPLQLLVT
                     SLGYDEYRGITAVGRIFAGQIKVGQALARIKVDGDMLPERARYLYVHQGLARIEVDQA
                     QAGEIVALAGLEAIAIGETLADPVNPQALPTIRVEEPTVRMTFGVNTSPFSGREGRWS
                     TSRKLRERLYEELRTNVALRVTDTSNADTLLVSGRGELHLAILIETMRREGYEFQVSR
                     PEVIMRQGEQGEMLEPFEEVHIETSPESVGVVVEMLGARRGQMLNMQNSAQNSVYLKF
                     LAPIRGLIGFRYQFLSATRGAGVMHTLFHGYLPHAGPISGRSSGSLVSWETGVATTYG
                     LKNAEERGVLFCDPGTEVYEGMVVGEHQRPGDLSVNVCKKKHLTNMRAARGDMEIRLT
                     PPRRMSLDEAIEYLAEDELLEVTPLEFRLRKRVLSSHERGRLNKLEKEAPIRVLAQ"
     gene            complement(5983..>6507)
                     /locus_tag="AC16V7_17455"
     CDS             complement(5983..>6507)
                     /locus_tag="AC16V7_17455"
                     /inference="COORDINATES: protein motif:HMM:NF012523.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatA-like O-acetyltransferase"
                     /protein_id="HIR6258966.1"
                     /translation="VKRSGIPITVAIVYVLSRAANAIPEFRYRLRGENVIEHEIVHPS
                     ITLLVDDNLFSFCTFDYVERFSEFSAKAIEKMAYVKAHPTLEDEGELDNLLFMTALPW
                     VSFTSFMHPMQLHPADSVPRFAWGKFFKDGDLLKMPLGVQGHHALMDGIHMGWCYEKV
                     QDYLYHPGFVVGEA"
BASE COUNT         1617 a         1636 c         1746 g         1508 t
ORIGIN      
        1 actaggaatg atgttcggta gttttacata catctattga ctgagccatc tggcacaatg
       61 gcaaaccaag ttgcccttcc agttcttact catcctgggg atgacgtaat cgtaagccga
      121 gaaagccacg ccgtctggca tgagactggc gcctcggcag cgaactcggg cgtacagttc
      181 accgctatcg gagcaaaagg tgcgtttacg ctcgatgagt ttttagaagc catcaaaccg
      241 cgcggccatg tcatatatcc acccaccacc ctggttgaga tagacaatac tcacaaccgg
      301 gcgggaggag tcatcttccc tgatagtgtt tgcactcatc ccacgcacca ttcgggtagt
      361 gacacacctt tccagccaac atggcttagc tatgcggcgg aacctaaacg ttaggcggag
      421 agaattcatt tcaatgaaca gccatcttaa tcggatacat cacttcaagg gcaatccacg
      481 tgaaattgga cttgcggctg ggcgcttgct cggcgataaa ctcgcacaga acatcaatca
      541 ctacatcggt agcctagaac attccaagga catggaagaa ctacatactg gcgccttacc
      601 ctggttgcgt cgtttgccgg aacgatttca ggaggaatat gaaggactgg cagaaggggc
      661 aaacatcccg ctgcaacgcc tggctgaatg ggggtatctt gaggcatgca aatctataca
      721 gtgcagtggg acagtgtatt tatttgaaaa tcaagcctgg gttgcgcgca ataatgattt
      781 ttatttgccc gagttatggg gctatgtaac ggttagagaa gttgacggac ggattccaac
      841 gatcaatttc cccatggaag gggacgtgtt cacgccaact ggcgtcaata aagagaaatt
      901 gtggttgcac tataactacc tgcctgtttg ggataaccca tcccctaata aaccgcatgt
      961 ccctgcctat gtgttcttaa ccgaagcgct ggagatctgc cacactatcc gcgatgtcga
     1021 gacgtttctc aatgaaatgg atagagatgg tggcatgtta ttatttgctg tcgatggaaa
     1081 gaacaatgag ttcgctcttt tcgattgcat gtgctcaaaa cactatcgac gggaaccatc
     1141 cgatggctgg attgttggga ccaatcatta ttgtgccgtc gaagacctga ctttgggcga
     1201 tgatgaagac tcagccaaca cgataaatcg atttagacgt atggaaagcc tcatgcaaga
     1261 cttagctacc tcgaagacac ccccgagtct acccaccgat ctcatcaaga tcctggcgga
     1321 tgatgagatc gaaaggcgtg acgtagaggt aattactgtg tattctaacg tggcatgtcc
     1381 gagcacaggt gagatctggt tcacatttgg aggacaccca gcagcaagcc aagggaattg
     1441 gcaaaaaatt gactggccgt ggaaagattg ataagaattt tttattgaga taagggatac
     1501 aaagtatagg atgaaatagt gatatcttcc gaagctccca tagttgcacg aatgcagtcg
     1561 ctcattcacc aatgggagga aacatccaat ccccaagcgg tgtttctgag ctgctacaaa
     1621 atgatgacga gcaatatgct tacctctatc gagcgacagg aattcatgga tcccccatgg
     1681 gtcaccaatc tactgcaccg ctttgctgac cactatttcg tagcgctaga atcctatgag
     1741 aaggaaccga cgtctgcccc acgcgtctgg cagttggcgc atgacaccac tcgcgacccc
     1801 caagtgctgc cgcttcaaaa cctgctgctt ggcatcaacg cccacatcaa ctacgacctg
     1861 gtgataacgt tggttgactt gctgagccca gaatggaatg accactccga cgagcagcgt
     1921 gcctcgcgct actacgattt ttgtcatgtc aacgatgtca ttgtccgtac cattgacgtt
     1981 gttcaggacc aggtgttgga gcccgccatg ccggggatgg atattctcga caaactgctt
     2041 gggtctctcg acgaattgct ggtttcgcgc ctgattacaa gttggcggga gaaagtgtgg
     2101 cacaatgtac taagtctgct tgaggccact gcacaagacg agcaatggtc tttgcttcag
     2161 caaattgaag aagaagctgt cgggttaggc aagctgatca gcttgcgcga ttagcgcagc
     2221 ccgtctcaat atagaagaaa cttggcgctt caaaccgcta tgctacgtga agaacactct
     2281 gtcagatttt atcgggattt gtttttcgca actcggtcct aatgatgaat tggtgaaaaa
     2341 agcaactttt tagggggcga tggcaagcgc agtacattat ccgtcggatt tgcctcttga
     2401 caaacggata tatacgatat aaaatatcca taatagatat atcgttcacg gatatatata
     2461 cttccgtctt tggagcatca gtgactatta cctcccccct accagaagat aacctcccca
     2521 tgactccggc tatgttcaat atcctgctgg cgctggctga cggcgaaaag cacggttacg
     2581 ccatcatgct agaagtggaa gccaacaccg aaggggcggt caagatgggt cccgggacgc
     2641 tatacggctc gatcaagcgc atgctggcca gtggcctgat cgaagagtgc gacgagcgcc
     2701 ctgacccagc gttggatgat gagcgccggc gctattaccg cttaagtggt ttggggcagc
     2761 gcgccctgcg cctggaagcc gagcgcctgg aagggcaagt ccgcctggcg cgtttaaagc
     2821 aggtcctgaa aggatcagcc tgatggaggc aaagatggaa aaagtctcca gcgcatcacg
     2881 atcgttgatc ttgtccatcc gcctttacca ggcgctgctc actgtttacc cgtccgagtt
     2941 ccgccgggcg tatggcggtc ctatgctgca ggtatttcgc gactgcagcc agcgagcgct
     3001 gcatgaaggc ggcacagctg gtctgctggc tttgtggagc cggacgatgc tcgacaccgt
     3061 tcaaacggcg ctcgaggaac atatgcaacg aggtgtggat atgtcaaaag agaaattttt
     3121 caaactgagt ggttgggcat tgatgctcgg cggcctggca acgatgctgg gttggctggc
     3181 aagcacacgc ccggattaca accgctacaa tgcccgcagc ctgcctattg accagtacgc
     3241 caatgcgatc gaatttccac taattgtcat gggcatgctc ttgctgagcg ttggcttgat
     3301 cgggctgttc ctgcgctacg ggcagggggc tgggggtttt gggcggttta gcctggggct
     3361 gggcgcgctc agcggagcga tcagtgcaat cggcgccatt ggtctgggca tctttgacag
     3421 cgagccctgg tggtcaacct tcttcattgg tatggtggtt cagtacctgg gattgacact
     3481 gttcggcgtg gctaacttgc gccagcgcgc actgccgcgt tggaacgggc tgcccatctt
     3541 ggcaggtata tggatcccct tgtccgtcgt ggtgggcttg attattgaac aaggaacagg
     3601 tcaatgggtg gagtggcctg aagcggtttt tctaatcgtc tggctactca gcatggcagg
     3661 attggcaggc ttgggctacc tgatgcaatc tgacccacag cccgccgacc cggcagcagc
     3721 tatggcataa aagatttttg gccgaggtga caagcatcaa atatatccac tgggtaggtg
     3781 ggaaatgatc ttggcaatgt taattaaacg gaccaaaaag aagcaaacat tcgtagaccc
     3841 gcgcctgaat agacctgatg tagtattgcc cagactttag tcaaagggct gaatactgta
     3901 aatcaaaaat taatataccc cttctcaaat tggaggcttt ttggtaatat tgagtggctg
     3961 aaaggcgacc ccggcctgaa tggtcggggt cgttatctgt tcaaatatag aaactgagat
     4021 agatgtagaa agtttagcat agtatgttaa ttgaaagaac tgatcttcgc aatatcacta
     4081 tcattgccca cgtggaccat ggcaaaacta ccttggtgga tggtctgctc aggcaggcgc
     4141 gggtttttcg tgagaaccag catgtggtcg agcgtgtaat ggattccaac gacctggaac
     4201 gcgagcgcgg catcaccatc ctggcaaaaa acactgccat cgaggtgtgg gataacctgc
     4261 gcaagcaaac tgtaaaaata aacatcgtcg acacgcccgg acacgccgac tttggcggtg
     4321 aggtcgaacg ggtgatgaac atggttgatg gtgtgttgct gttggtggat gccgccgaag
     4381 ggcccatgcc gcaaactcgt tttgtgctca agaaagccct cgagatgggt caccgtgcaa
     4441 ttgtagtgat taacaaggtc gatcgtaaag atgccatccc ggaagccgtg ctcaaccaaa
     4501 cctttgacct gtttattgaa ctaggtgcaa ccgatgagca ggcagatttc ccggtaattt
     4561 atgccaatgc ggtaactgct cagtctggtc tgacgccggc attaagtccc gacctgcaac
     4621 atctcttcca cgctatcttg cgccatatcc ccgcgccgcg tgtcgacccg gatgcacccc
     4681 ttcaattact ggtaacctca ctgggctatg atgaatatcg cggtatcacg gctgtcgggc
     4741 gcatcttcgc cggacagatt aaggtaggac aagcccttgc acggatcaag gtggatggtg
     4801 atatgctgcc tgagcgcgcc cgatacttgt atgtacatca aggattagcg cgtatagaag
     4861 tcgatcaagc ccaagcaggc gagattgtcg ccctggcagg attggaagcc atcgccatcg
     4921 gcgagacact agccgatccg gtgaatccgc aggcactgcc taccatccgg gtcgaggagc
     4981 caaccgtgcg catgaccttt ggcgtaaata cctcaccttt ttctgggcgc gaagggcgtt
     5041 ggagcacctc gcgcaagctg cgtgagcgat tgtatgaaga attgcgcacc aacgtggcgc
     5101 tgcgcgttac ggacaccagc aatgctgata cactcctggt atcaggtcgg ggagagctgc
     5161 acctggctat attaatcgaa accatgcgcc gagaagggta cgaattccag gtctcgcgcc
     5221 cggaagtgat catgcgccag ggggagcaag gggagatgct tgagccattc gaggaggttc
     5281 acatagaaac cagccccgaa tcggtgggtg tcgtggtaga aatgctgggt gcgcgccggg
     5341 gtcaaatgct caatatgcaa aacagcgccc aaaacagcgt atatctgaag tttttggcgc
     5401 ccatccgcgg gctcataggc ttccgctacc agttcctctc cgccacgcgg ggcgcaggtg
     5461 ttatgcacac cttgtttcat gggtacttac cccacgcagg cccaatcagc ggacgatcgt
     5521 ctggctctct tgtatcctgg gaaaccgggg tcgccacaac ctacggattg aagaatgcag
     5581 aagaacgcgg cgtgctattt tgtgatccgg gtaccgaggt ctatgagggt atggtagtag
     5641 gcgaacacca gcgacccggc gatctatctg ttaatgtgtg caagaaaaaa cacctgacga
     5701 atatgcgcgc tgcccgaggt gacatggaaa ttcgcctcac ccctccccgc cggatgagcc
     5761 tggatgaagc catcgaatac ctggctgaag acgaactact ggaagttacg cctcttgaat
     5821 ttcgccttcg caagcgcgtc ctctcaagcc acgagcgcgg gcggctcaat aaattggaaa
     5881 aagaggcccc catcagagtg ctggctcaat aacggtttga cccagttgtt ggaagccaga
     5941 tcaaggatga ttatcccaac aggtagggct taggctgcgc acttaagctt cacccactac
     6001 aaaaccggga tggtacaagt agtcttgcac tttttcatag caccacccca tgtgtattcc
     6061 atccattagt gcgtgatgtc cttgcacacc caacggcatc ttcaacaagt ctccatcctt
     6121 aaaaaacttc ccccatgcga agcgtggcac agagtccgct ggatgcaact gcattgggtg
     6181 catgaagctg gtgaacgaca cccagggtag cgcagtcatg aacaacaagt tgtctagttc
     6241 tccttcgtct tccagtgttg gatgggcttt tacataagcc attttctcga tcgctttagc
     6301 agaaaactcc gagaacctct caacataatc aaacgtgcag aagctgaaca aattgtcatc
     6361 caccaagagc gttatggatg gatgcacgat ctcatgttca atcacgtttt cccctcgaag
     6421 gcgatagcgg aactcaggga tcgcgtttgc cgctcgagag agcacgtaaa cgatagccac
     6481 ggtaattggg atgccagatc gcttgac
//
LOCUS       DBNHOH010000965         2321 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1728602, whole genome shotgun sequence.
ACCESSION   DBNHOH010000965 DBNHOH010000000
VERSION     DBNHOH010000965.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1728602"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="AC16V7_17460"
     CDS             complement(<1..222)
                     /locus_tag="AC16V7_17460"
                     /inference="COORDINATES: protein motif:HMM:NF013183.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HIR6258967.1"
                     /translation="MTLRIGILTVSDRSSQGERADTSGPAIQAVAKQQGWVVTKTGII
                     PDHLEAIQEMLIDWVDSEEVDVILTTGGTG"
     gene            complement(219..659)
                     /locus_tag="AC16V7_17465"
     CDS             complement(219..659)
                     /locus_tag="AC16V7_17465"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6258968.1"
                     /translation="MIEGDELAFKIREFQYPQDYPAVSALWEASGPGVIPGRSDTPEE
                     TQKKLGRDPDLFLVAEVDDHIVGTVLGGFDGRRGLVYHLAVAKEYRGRGLGDSLMNEV
                     ESRLKEKGCIRAYLLIAHDNGGVVNFYKRRAWETMNLTVMAKDL"
     gene            complement(652..>2321)
                     /locus_tag="AC16V7_17470"
     CDS             complement(652..>2321)
                     /locus_tag="AC16V7_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HIR6258969.1"
                     /translation="QPGVSYLEFLQRAESRGLDIVSMTDHNTVSGYRRMKEEIHQLEL
                     LEKLNRLLPDEQKRLDEYRRLLKKILVLPGFEFTATFGFHIMAVFSPDKPVREIEHLL
                     LDLRIPPDQLDDGSATVGATSDVLTAYRLIAEAGGLVIAAHANSSNGVAMRGFGFGGQ
                     TKIAYTQDPNLHALEVTDLEQKGKRNTAAFFNGTKPEYPRRMHCIQGSDAHRLTADPQ
                     RKKNLGMGDRAVDVLIPEISFEALMELFTSNDFARTRPHRHTEEPAFDFIHAAREEGA
                     NIVQDFHEGLAVRGGQRYAIIADVCAFANANGGTLYIGLSEDPNKNVVGVPDPEHAIS
                     QLEKEISNRISPPLHCSLDVHETEAKKIVRILVPGGDDPPYAVDDNKIYVRSEAETAL
                     AVRDEIVGLVQRGIPRQIPAVEAEEFQAVSKAEVSLPEAPAQVEEEAPPRTGVEVVAV
                     EEREGARYYTMRDLRNGNVVKNVTRTSARRLWHYAITRFSRYPVDLSQAGIQWQGNLG
                     LVHTQKQDKSVRYDLVQRTTQGYRYYFGVTDDGIHGPWANLVGQEDD"
BASE COUNT          527 a          661 c          577 g          556 t
ORIGIN      
        1 gcctgtccct ccggtggtca aaatgacgtc cacttcctcc gaatcgaccc aatcgatcag
       61 catctcctgg atggcttcta agtggtcggg aatgatgccg gtcttcgtca ccacccaccc
      121 ctgctgcttt gcgactgcct gtattgctgg tccagaagta tcagcgcgct ctccttggga
      181 ggagcgatca gaaacggtca agatgccgat gcgtaaggtc ataaatcctt cgccatgacc
      241 gtgagattca tcgtctccca ggcacggcgc ttgtaaaaat tcacaacacc cccgttatcg
      301 tgagcaatca gcaaatatgc ccggatgcag cctttttctt tcagcctgct ttctacttca
      361 ttcatcaaag aatcccccaa gcctctacca cggtactctt tggctaccgc gagatggtac
      421 acgaggccgc gccgtccatc aaagcctccc aagacggtgc cgacaatatg atcatccact
      481 tcagccacca ggaaaaggtc aggatcgcgt cctagttttt tctgcgtctc ttccggggta
      541 tcggatcgcc caggaatcac cccaggtcct gaggcctccc ataaagcact gaccgctggg
      601 taatcctggg ggtattgaaa ttcacggatt ttgaaagcca gttcatcacc ctcaatcatc
      661 ttcctgtcct accaggttag cccaaggacc gtggataccg tcatcggtga caccaaagta
      721 gtaccgataa ccttgagtag tgcgctgcac aaggtcatag cgaacactct tatcctgctt
      781 ctgagtatgc accaggccca gatttccttg ccactgaata ccagcttggg atagatcaac
      841 aggatagcgt gagaatcggg tgatggcata gtgccacaga cggcgcgctg atgtacgcgt
      901 gacgtttttc accacatttc cattgcgcag gtcgcgcatt gtgtaatagc gcgccccctc
      961 acgctcctcc acggccacaa cttcaacccc cgtgcgcgga ggcgcctctt cttctacctg
     1021 agcaggtgct tcaggtaatg atacttcggc tttcgataca gcttggaatt cctccgcctc
     1081 tactgcgggt atttgcctcg gaatccctcg ctgaaccaaa cccacaattt catcccgcac
     1141 cgccagggca gtctcggctt cagagcgcac atagatctta ttgtcatcga cagcatatgg
     1201 aggatcgtca ccaccgggaa cgaggatgcg gacgatcttc ttcgcttcgg tttcgtggac
     1261 atcgagcgag caatgcaggg gtgggctgat gcggttgcta atctccttct ccaattggga
     1321 gatggcgtgc tctggatcgg gcactcccac cacgttttta ttcggatcct cgcttaggcc
     1381 gatatacagc gtaccgccat tggcattggc aaaggcgcat acatcggcga tgattgcata
     1441 acgctgtcca ccgcgcaccg ctaagccctc atgaaaatcc tggacgatgt tggcgccttc
     1501 ttcccgggca gcgtggataa aatcaaatgc tggctcttct gtgtggcgat gcggacgggt
     1561 gcgggcaaag tcattgctgg tgaataactc catcaaggct tcgaaactaa tctctgggat
     1621 gagtacgtca accgctcgat cacccatacc caggtttttc ttccgctggg ggtcggctgt
     1681 cagacgatga gcatcagagc cctggatgca gtgcatacgc cttgggtact ccggtttggt
     1741 gccgttaaag aaggctgcag tgttgcgttt acccttttgc tccagatccg tcacttccag
     1801 ggcatgcaaa ttgggatctt gagtgtaggc gattttggtc tgcccgccaa aaccaaatcc
     1861 gcgcatagcg acgccgttgc tggaattggc atgtgcagca atcaccagac caccagcttc
     1921 agcgatcagt cggtaagccg tcagcacatc gcttgtcgca ccaacagtag ccgatccatc
     1981 atctaactgg tcgggcggga ttcgcagatc tagcaaaaga tgctcgatct cacgcaccgg
     2041 cttatctggt gagaaaacag ccatgatatg aaagccaaaa gtggcagtga actcaaagcc
     2101 aggcaatacc agtatttttt taagaagacg gcggtattcg tccaatcgct tttgctcgtc
     2161 tggcaagagt cgatttagtt tctctaaaag ctcgagttgg tgaatctctt ctttcatgcg
     2221 ccgatagccg gaaacagtat tatggtcggt catagaaaca atatccagac cgcgggactc
     2281 agcgcgctgc aagaactcta ggtagctaac cccaggctgt a
//
LOCUS       DBNHOH010000966         6118 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1536434, whole genome shotgun sequence.
ACCESSION   DBNHOH010000966 DBNHOH010000000
VERSION     DBNHOH010000966.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6118)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1536434"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1454
                     /locus_tag="AC16V7_17475"
     CDS             <1..1454
                     /locus_tag="AC16V7_17475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258970.1"
                     /translation="QYLIERHERDIFPILRRRHIFAEAKNFLLYDFFTPQGNVNEDVF
                     AYSNRSGDERALVIYNNKYASTNGWIRTSSFYLLKSGDSTDSSPDSGGGASTTSRQLV
                     QKNLGAGLELTDDPNTYTIFSDQISGLEFIRNNHDLHQNGLYIELGAYQYHVFLEFRQ
                     VQDDDWHQYSQLNTYLNGRGVPSIDEALKQILLQVVQHPFRELFNPGFIHWMRDQDTE
                     LPTALDDFESRVNRLLNGVRTIAGGESELGSIASIIRADAEALLRLTRLPDRMPARTS
                     AKTMAAINYLLSGPGEELPLDEDNQFVWVILYAWIICRRLGEVVNPLAAGEISRSWIE
                     EWQLGKMMAEMLREMGNDDASIESAVTLVKLLCSHQVFSGELDLGAGAERGLLYTNWL
                     RTWLQDVELQRFLKFNRYQGFLWFNRETFEDWLWWNLATAMIDLTSGIPETIEIETRK
                     SIATRIDIVRRLLKAAESSKYQVEILLEKARGK"
     gene            complement(1535..2941)
                     /locus_tag="AC16V7_17480"
     CDS             complement(1535..2941)
                     /locus_tag="AC16V7_17480"
                     /inference="COORDINATES: protein motif:HMM:NF015052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HIR6258971.1"
                     /translation="MQSFSIHAHFYQPPREDPFTGIIPIESGAAPFINWNERIHSECY
                     RPNAELGNFERIGYNIGPTLFEWMAAYDPIASQRIVAQDRANIDRYGVGNAIAQPYHR
                     TILPLATRYDKKTQIVWGIEQFKHRFGRKPQGMWLPETAVDEESLITLVDQGIEFTIL
                     APWQAEDDNLDVTEPYRVRLPEGREITVFFYHGWLSGKVSFDPMATMNADQFVRLHLK
                     PHFRPEKIQRGEPQLLMIASDGELYGHHQSLRDHFLAHLVNGAASQESLATTFPALWL
                     REHPPRQSVKIRERTSWSCIHGVARWVGSCSCVPGNQGWKSHLRHALDRFSQQMDVLY
                     HTAVSPFVKDPWALRDDYIRVLLGLQPVKDLIAQASGKRLPEEICVRIQILLESQLER
                     QKMYASCGWFFEDFERIEPRNSVAYAAKALHLTNRATGVDLAPKLMANLEKVVSSRAG
                     LRGDQLLNDYLSRSVRSK"
     gene            3077..4597
                     /locus_tag="AC16V7_17485"
     CDS             3077..4597
                     /locus_tag="AC16V7_17485"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033840 - alpha-1,4-glucan
                     glucosyltransferase (NDP-glucose donor) activity [Evidence
                     IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HIR6258972.1"
                     /translation="MKYPIKVLFLAAEANPFIKVGGLADVAGSLPHTLRSLRVRSTTR
                     DASANSDEIPAVDVRLVIPLHNAIRNRNLPLKVTTEFTIEHAQGPIPVQVFETDRNGM
                     IVYLIASELISDDEQLYHADASQDGPKYTLFSLAALELVRKLKWSPDVLHANDWHTAL
                     AVYALDILRSSERFFKKSISVLGLHNLPYLGVGAGDALAPFGLTPAYGSALPWWAQDM
                     PLPMGLLTANHIVTVSPGYAQEILTPEYGSGLDEFLQSRSQDITGILNGIEIQDWDPN
                     SDLTLAANYSLDRLESRRANKAALQNELGLPIDPDIPLLTMVTRLDPQKGVDLLPNAL
                     RQVADLAWQAVILGTGTPSLEGMMRALETSFPDRLRAVVRFDSKLSRRIYSAADAILI
                     PSRYEPCGLTQMIAMRYGCIPIAHATGGLRDTIQDYADFSHSTGFLFQGATSENLGSA
                     LRRALRVFSERELWHAMQIRGMQKDFSWESSAKQYLDLYVSLITSYGKRKLQQVNK"
     gene            4594..5856
                     /locus_tag="AC16V7_17490"
     CDS             4594..5856
                     /locus_tag="AC16V7_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase family
                     protein"
                     /protein_id="HIR6258973.1"
                     /translation="MKTRAVILAGGEGSRLGILTAKRTKPAVPFAGKYRIIDFTLSNC
                     VNSGLFDIMILAQYRPHSLIDHIGAGNPWDLNRDFTGGVRIFTPYKARGSSDWYLGTA
                     DAVQQNFRFIKSGDPELILILSGDHIYEMNYDALIAFHLDHDADLTMATIRVPISDAS
                     RFGIVGVDNRFRVTTFVEKPAQPPSNLVNMGVYVFNFKALDQALLDDSEKTGSSHDFG
                     KDILPRMIQNQARVFAFPFSGYWVDVGTVESYWQAHMDLLYDNPPFDLNDRSWIIHTR
                     TEERPPAWISGGAVVIDSMITDGCVIAPGARIERTVLAPGVRVHAGAIVRESVLLTDT
                     LIEEGAVVERAVIDKRAVIGESARVGRSQPDEIPKIAMIGKNSYVPPGYIIEPGAVIA
                     TDVAQEDYPSKIIREGDYIQTKRPPYEV"
     gene            5846..>6118
                     /locus_tag="AC16V7_17495"
     CDS             5846..>6118
                     /locus_tag="AC16V7_17495"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HIR6258974.1"
                     /translation="MKFERASGILLHPTSLPGPYGIGDIGSGAHLWLEFLAKTGCSLW
                     QILPLGPTGYGDSPYQCFSAFAGNPYLISPEALLNDGLLFPDDLADI"
BASE COUNT         1552 a         1549 c         1488 g         1529 t
ORIGIN      
        1 atcaatatct gatcgagcgc catgaacggg acatcttccc aattctgcgt cgacgccata
       61 tctttgctga ggcgaaaaac ttcttgctct acgacttttt taccccgcaa gggaatgtca
      121 acgaagatgt tttcgcttat tcgaatagat ctggcgatga gcgggcgctt gtgatctaca
      181 acaacaaata tgcttcaaca aacggatgga tacgcacttc atctttctac ctgctgaagt
      241 ctggcgatag taccgatagc tcacctgatt caggtggagg cgcttccaca actagcaggc
      301 agctagttca aaaaaacctg ggagctggct tagaactaac ggatgacccg aacacctata
      361 caattttctc cgatcaaatc tccggtctag agttcatccg aaacaaccac gatctccacc
      421 aaaacggact ctatatcgag ctgggcgctt accaatatca cgtttttctg gaatttcggc
      481 aggtgcagga tgatgattgg catcagtata gccagctcaa cacatattta aacgggcgtg
      541 gtgtgccatc tattgatgag gcgcttaagc agattttgct acaagttgtg caacatccat
      601 ttagagaatt attcaatccc ggtttcattc attggatgag agaccaagac actgaactgc
      661 caaccgccct ggatgatttt gaatctcgcg taaatcggtt attgaatggc gtccgcacga
      721 tagcaggagg agaatctgaa ttgggttcta tcgcctcgat tattcgcgcc gatgcggagg
      781 cgctgctgcg tctcacccgt ctacccgatc gcatgcctgc aagaacgagc gcaaaaacga
      841 tggccgcaat aaactatttg ctatctggac caggggaaga attgccgctg gacgaagaca
      901 accaatttgt atgggttata ttgtacgcct ggatcatttg ccgaagactt ggagaggtcg
      961 taaatccctt agctgcggga gagatcagcc gttcttggat tgaagagtgg caactcggaa
     1021 agatgatggc tgaaatgcta cgagagatgg ggaatgacga tgcatctatc gagagcgctg
     1081 tcaccctagt taaattgctt tgcagccatc aagtgttctc tggtgaactt gatcttggag
     1141 caggagccga aagagggttg ctatacacta attggcttcg aacctggctc caagatgttg
     1201 agctacagcg ctttcttaaa ttcaaccgct accagggttt cctatggttc aatcgagaga
     1261 catttgaaga ttggttatgg tggaatcttg ccactgccat gatcgatctc accagtggaa
     1321 ttcccgagac catcgagatt gaaaccagga aaagcattgc gacgcggatt gatattgtca
     1381 gacgcctact taaagccgca gagagtagca aatatcaggt cgagatacta ctcgaaaaag
     1441 ctcgagggaa atagcatttc tttattttct tttttacttc atggtaaatg ctatgaaacg
     1501 gcatctatta cacatactga tcagtttatt gtgatcattt agatcgtaca cttctgctca
     1561 aataatcatt taagagctgg tcgccccgca agccggcgcg agagctaacc actttttcaa
     1621 gattggccat taacttaggc gccaaatcga ccccagtagc cctgtttgtc aagtgcaaag
     1681 ctttggctgc ataagccacg ctattgcgcg gctctattcg ctcaaaatcc tcaaaaaacc
     1741 atccacaaga agcatacatc ttctggcgct cgagctgaga ttctaaaaga atctggatgc
     1801 gcacgcaaat ctcctctggc aatcgcttcc cagatgcttg agcgattaaa tctttaactg
     1861 gctgcaaacc gaggagaacc cggatgtaat cgtcgcgcaa agcccaggga tctttcacga
     1921 aaggtgacac agctgtgtga taaagcacgt ccatttgttg tgaaaaccga tctaaagcat
     1981 ggcgcagatg agatttccag ccctggttac ctggcacaca ggaacaacta ccaacccagc
     2041 gtgccacacc gtgaatgcag ctccaggatg tgcgctcacg aatcttgaca gattgccgcg
     2101 gtggatgctc ccttaaccat aaagccggga aggtggtagc cagactttcc tggctcgccg
     2161 caccgttgac aaggtgagct agaaagtggt cacgcagcga ttgatgatga ccatatagct
     2221 ctccatctga ggcaatcatc aatagctggg gctctccgcg ctggattttt tccgggcgga
     2281 aatggggttt caggtgcagg cggacaaatt gatcagcatt catcgttgcc atcgggtcga
     2341 agctaacttt gccgctaagc catccatggt aaaaaaagac cgtgatctct cgcccttctg
     2401 gcaagcgaac acggtacggt tcggtcacat cgagattatc atcctcagct tgccagggag
     2461 ccaggatcgt gaactcaata ccttggtcaa cgagggtgat cagagattct tcatctaccg
     2521 ctgtctccgg cagccacatg ccttgaggtt ttcgcccgaa acggtgcttg aactgctcga
     2581 taccccaaac gatctgcgtc tttttatcgt aacgggtggc taaggggagg attgtacgat
     2641 ggtaaggctg agcgatggca tttcccacac catatctgtc gatattggcc ctgtcttgtg
     2701 ccacgatgcg ctgggaggcg attggatcat aggctgccat ccactcaaaa agggttggtc
     2761 caatattgta gccaattcgt tcaaagttac ctagctcggc gtttggacgg tagcattcag
     2821 aatggattcg ttcgttccag ttaatgaaag gtgccgcccc agattcaatt ggaattatcc
     2881 ctgtaaaagg gtcctctcgg ggcggctgat agaagtgggc gtgaatactg aatgactgca
     2941 atggatttct cagattatgt ttatcatata tattatattc gattatgtga tttaaaccac
     3001 ataatcgtgt aaaattgatt ctacccatcc tcttgtgctc agaaacaagg acaggaatct
     3061 cgcagacaat actctcatga agtatccaat caaagttctc tttctcgcgg ctgaggccaa
     3121 cccattcatt aaggtcggtg ggctggcaga tgtagccggt tccttgccac atacgcttcg
     3181 ttctctgaga gtacgaagta caaccaggga tgcttcggca aattcagatg aaattccagc
     3241 tgtcgatgtg cggctggtaa ttccgctaca caatgccatc cgcaaccgaa atttaccgct
     3301 gaaagttacc accgaattca ctatcgagca tgctcaaggg ccgatcccag tgcaggtttt
     3361 tgaaaccgat cggaatggca tgatcgtcta cctgattgcc agtgagttga tcagtgacga
     3421 cgagcagctc taccacgccg atgcaagcca ggatgggccc aaatacacgt tgttctcact
     3481 cgccgctctc gaactagtcc gcaaattgaa atggtcgccc gatgtattgc acgccaacga
     3541 ttggcacaca gccttggcag tttacgccct agatattttg cggagctcag agcgcttttt
     3601 taagaaaagc atctccgtac taggtctcca caacttgcca tacctgggtg tgggagcagg
     3661 agatgcactg gcaccctttg gattaacacc cgcttacggc tccgctctcc cctggtgggc
     3721 gcaggatatg cccctaccga tgggcttgct tacagccaac catatcgtga ctgtctcacc
     3781 tggttatgcc caggaaatcc tcacccccga gtatggttcg ggactagacg aattcctcca
     3841 atcgcgctcc caggatatca ctggcatctt gaacggcatt gaaatacagg attgggatcc
     3901 aaactcagac cttaccctag cagccaatta ttctctggac aggctagaat ctcgtcgcgc
     3961 caacaaagcc gccctgcaaa atgagctggg cttgcccatc gaccctgata tccctctgct
     4021 cacgatggtc acccgactgg atccgcaaaa aggcgtcgac ctgctgccca atgcgctgcg
     4081 ccaggtagct gatctcgcct ggcaagcagt gatcctgggg acaggcaccc cctccctcga
     4141 agggatgatg agagccttag agaccagttt tccagatcga ttgcgagctg ttgtccgctt
     4201 cgactcgaag ctaagccgac gtatttactc cgcggctgat gcgatattaa tcccttctcg
     4261 atacgagcct tgtgggctaa cccagatgat cgcaatgcgg tacggttgca tcccgattgc
     4321 tcacgccacg ggtgggctga gggacacaat acaggactat gccgatttta gccacagcac
     4381 tggatttctt ttccagggag ctacttccga gaacctcggc tccgccctac gccgagcttt
     4441 gcgtgttttt tcagaacgcg aactctggca cgcgatgcag atacgcggta tgcaaaagga
     4501 tttttcttgg gaaagttcag ccaaacaata tcttgatctc tatgtatctc taataacgtc
     4561 ttatggtaag agaaagttac aacaggtgaa taaatgaaaa cacgtgcagt cattctggct
     4621 ggtggagagg gttcacgcct tggaatactc accgccaagc gcacaaaacc ggctgtcccc
     4681 tttgcaggta aataccggat tattgatttc acactctcaa actgcgtcaa ttctggtttg
     4741 ttcgacatca tgatcttggc tcaataccgg ccccattcac ttatcgacca tatcggggca
     4801 ggaaatccat gggacctcaa tcgcgatttt acagggggtg tgcgtatatt taccccttac
     4861 aaggcgagag gctcatcaga ctggtatcta ggcacagcag acgctgtgca gcaaaacttt
     4921 cgctttatca agagcggcga cccagaactg atcttgatcc tatccggtga ccatatttac
     4981 gagatgaatt atgacgcgct gatagcattc cacttggatc atgacgcgga tctaacgatg
     5041 gctaccatcc gcgtgccaat atcagacgct tcacgttttg gtatcgttgg cgtagataat
     5101 cgatttcgag ttacaacatt cgttgaaaag ccagcccaac ccccttcaaa cctggttaat
     5161 atgggtgtat acgtatttaa ctttaaagcc cttgaccagg ctcttctgga cgatagcgag
     5221 aaaaccggaa gctcacacga ctttggaaag gatattctcc cgcgcatgat ccaaaaccag
     5281 gctcgcgtat ttgcatttcc ctttagtgga tactgggtag atgtggggac ggttgagtcg
     5341 tactggcaag cacatatgga cctgctatac gataacccac ccttcgacct gaacgatcgt
     5401 tcatggatca tccatacccg tacagaagaa cgccctccag catggatatc aggtggtgca
     5461 gtcgtgattg acagcatgat caccgatggc tgtgtgattg cgcctggagc gcgtatagaa
     5521 cgcactgtgc tagctccagg cgtgcgggtt catgcgggtg caatcgtccg agaatctgtc
     5581 ttgctgacgg atacactcat cgaagaaggg gctgtggtag aacgcgctgt catcgataaa
     5641 cgcgcggtta tcggagaatc tgcccgcgtt ggacgcagcc agcctgatga gataccaaaa
     5701 attgccatga tcggcaagaa tagctatgtg ccaccaggat acatcatcga accaggtgca
     5761 gtgatcgcga ctgatgtcgc ccaagaggat tatccatcga aaatcatccg cgagggtgat
     5821 tatatacaaa ccaagaggcc gccttatgaa gtttgaacgt gcttccggca tccttctcca
     5881 ccctaccagc ttgcctggac cttatggcat cggcgatatc gggtcaggcg ctcacctgtg
     5941 gttagaattt ctggcaaaaa ccgggtgctc cttgtggcaa atcttaccct tggggcccac
     6001 cggttatggc gattcgcctt accagtgttt ctccgccttt gctggcaatc catacttgat
     6061 aagccctgaa gctttgctaa acgatgggtt attgtttccc gatgacttgg ctgatatt
//
LOCUS       DBNHOH010000967         3113 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1664659, whole genome shotgun sequence.
ACCESSION   DBNHOH010000967 DBNHOH010000000
VERSION     DBNHOH010000967.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1664659"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(284..967)
                     /locus_tag="AC16V7_17500"
     CDS             complement(284..967)
                     /locus_tag="AC16V7_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR6258975.1"
                     /translation="MTKFTWYGHATMGLETGGYKLLIDPYFSENPAATTTVDQVQADY
                     ILVSHGHFDHVTDLLEVARRTEATIITNFEISNWLGQQGLNSHAQHIGGGFKHPFGYL
                     KLTLALHGSGLPDGFYGGNPAGFLLTTLDGKKVYLACDTGLFGDMRLIGEEGVDLAVL
                     PIGDNFTMGPDDALRAVKLIQPQHVIPVHYNTWPLIAQDPQAWASRVKAETSAQVHIL
                     NPGESFTLP"
     gene            complement(1236..1691)
                     /locus_tag="AC16V7_17505"
     CDS             complement(1236..1691)
                     /locus_tag="AC16V7_17505"
                     /inference="COORDINATES: protein motif:HMM:NF013234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6258976.1"
                     /translation="MELKSAYPRPIVVPPGSGKVLEFLGVTHKLTSHHTGGAYYLFEA
                     EFDPESGNRLHVHRYEDEVVYVIEGAIEIRLDNQKLKASAGGVAHLPKNIPHALYNPL
                     KTPLRITAIAIPGGMENFFDELAVAYEAGTLDNAAHQKISQKYGIEWLE"
     gene            complement(1911..2345)
                     /locus_tag="AC16V7_17510"
     CDS             complement(1911..2345)
                     /locus_tag="AC16V7_17510"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR6258977.1"
                     /translation="MAVKLQVSIVIDRPVANVFHFYAHEHVRNHPRWDPDMQLEQISD
                     GPIGLGTLIRRRNTHSGTPVDGMMEVVEFEPDRAFGVVIQDGPSETHGRVTFKAESPK
                     QTTLTIHADFPDMDESLEAWITSMVERSARNIKQLIESEVPE"
     gene            complement(2485..2913)
                     /locus_tag="AC16V7_17515"
     CDS             complement(2485..2913)
                     /locus_tag="AC16V7_17515"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR6258978.1"
                     /translation="MSEENKAIIRRFYEELDKGNFDIYDELCTPDFISHFPGNPQPID
                     RETRKQTSRMFYAAIPDCKHTLEDTIAEGNKVSFRGSARGTHTGAFQGQPPTGNSVMF
                     TGMRFYRIVDGKIAEEWASFDSLALMQQLGVIPALEESKE"
BASE COUNT          667 a          789 c          845 g          812 t
ORIGIN      
        1 cacatctcgc gtcgttatcg tgagcgcgat gtgttagccg aggcgcaggc gattttcccc
       61 catacccgcg tggcgcggga ttttgatcac tttcagatcc gccgggggga gttcatcaag
      121 atgaatgaaa aaccataacc ggttgaagag aatctaaact catttgtgcc cgctccctcc
      181 ggtcaatagg aagaagaagg gcaggtccaa tgcaagatcg aggtttgtaa aaacctgtac
      241 aaacctcgat cttgttttta tgcaggttgg ctatacttct cgatcagggt agggtgaaac
      301 tctcacctgg attgaggatg tgcacctgcg cgctggtctc ggccttcacc cgactggccc
      361 atgcctgagg atcctgggcg atgagcggcc aggtgttgta gtgcacgggg atgacgtgtt
      421 ggggctggat caatttgacg gcgcgcagcg cgtcatcggg gcccatggtg aagttgtcgc
      481 caatcggcaa caccgccagg tcgacgcctt cctctccgat caggcgcatg tcgccaaaca
      541 ggccagtatc gcacgccaaa tacactttct tgccgtccag cgtggtgagc aagaaaccag
      601 ccggattacc gccatagaaa ccatccggca agcccgagcc atgcaaggcc agggtcagct
      661 tcaggtagcc gaatggatgc ttgaaaccgc cgccaatgtg ttgggcgtgt gaattcaagc
      721 cctgctgacc cagccagttg gagatctcga aattggtgat gatggtcgcc tcggtccgcc
      781 tggcgacttc gagtaggtcg gtgacatggt cgaagtgccc gtggctgacc aggatatagt
      841 ccgcctggac ttgatccacg gtggtggtgg ctgctgggtt ctcagagaaa taggggtcga
      901 tcaacagttt ataaccgccg gtttccaaac ccatggtggc atggccgtac caggtaaatt
      961 tggtcataag gtaacctcct tgaaaataaa cctttgtgtg gaagccttga atggctgaag
     1021 ctttcttaac atgtaaagga ttctctagaa gatagtatat aactgaaggg catccggcgt
     1081 caaccaaaca tcacatatca gatcaggttg ttaaaaagta acggctggta cgggtacgaa
     1141 ggttaatcta taagcggtga ctgccaaagc cggtttcaat ttggtttggc aggaaaaatc
     1201 tcaccagtcc ggtccacact catgatggga tccgtctact ccagccattc tatcccatac
     1261 ttttgagata ttttttggtg ggctgcattg tcgagagtgc cagcttcata cgctacggcc
     1321 agctcatcaa agaaattctc catgccccca gggattgcaa tagctgtaat tcttaaaggc
     1381 gtcttcaaag ggttgtagag cgcatgggga atgtttttcg gcaagtgtgc tacaccacct
     1441 gcgctagcct ttaatttttg attgtcaagc ctgatctcga tagcgccttc tatgacatat
     1501 actacttcgt cttcgtagcg atgaacgtgg aggcgatttc cgctttcggg atcaaactcc
     1561 gcttcaaata gataatacgc gccgccggta tgatggctcg tgagcttgtg cgtaaccccc
     1621 aggaattcca atactttgcc cgagcctggc ggtacgacga tgggtcgtgg atacgcggat
     1681 ttcaattcca tctccttgac ttctcctttg gatgtgtgcg agatgagcat gtttggttat
     1741 ctgcggcttg aatgattggc tgctggtgga tcgcagttgt gaggctaatt cgtttcgatt
     1801 cggtccgctc tttggcgcct tgttaatgaa catccagggc gtcctatctc attgccatgt
     1861 tggtgccctg agcagtggat ttcaaacttc gtccgtcgcc cttctgccca ctattctggg
     1921 acttcggact cgatcagctg cttgatattc cgggcgctgc gctccaccat gctggtgatc
     1981 caagcctcca acgactcatc catgtcgggg aaatccgcgt ggatcgtgag ggtggtttgc
     2041 tttggacttt cagccttgaa tgtcacccgc ccgtgcgttt cgcttggtcc gtcttggatt
     2101 accacgccaa acgcccgatc tggttcgaac tccactacct ccatcatccc atcaaccggg
     2161 gtgccgctgt gggtgttgcg cctgcggatc aaggtcccta ggccaatcgg gccatctgat
     2221 atctgctcga gttgcatgtc tgggtcccag cgcggatggt tgcggacgtg ctcatgggcg
     2281 tagaagtgga aaacgttagc cactggtcgg tcgatcacta tcgaaacctg aagcttcact
     2341 gccattgttc tttcctttag gtttggcgtt gacaccgcct acataatctg agttcattct
     2401 aatgcttcag cttgatgcaa aagcccgagt gtatgtcttc atgcgctcct gggcattttg
     2461 tcacataaaa tccagccggt agctttactc tttactttct tcaagggctg gaatcacgcc
     2521 cagctgctgc atcagcgcca ggctgtcgaa gctggcccat tcttccgcga tcttgccatc
     2581 aacgatgcgg taaaagcgca tgccggtgaa catgactgag ttcccagtcg gcggctggcc
     2641 ctgaaatgct cccgtgtgag tgccgcgcgc agagccccga aaagagacct tgttgccctc
     2701 ggctatcgtg tcctccaggg tatgcttaca gtcgggtata gccgcataga acatgcggga
     2761 ggtttgcttg cgggtctccc ggtctatggg ctgggggttg ccgggaaagt gcgagatgaa
     2821 gtcgggggta cacaactcgt cgtaaatatc aaagttgcct ttatcgagtt cctcatagaa
     2881 gcgcctgatg atggccttat tttcctctga catcaaaagc ctcctttctg gcttaagcta
     2941 aatttgggta gtgttggtga ttctatgtgc tgagattaga cagggtagtt tgctgtcgct
     3001 cgaccaagtg cgggatgtgg gatatatatt ctatgctgcc tcatcaccaa gattaaccca
     3061 gggggtaaac gaccacagat taaaacgtgg ttcgtgtagc ttgtggtgtt atc
//
LOCUS       DBNHOH010000968         2695 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1215941, whole genome shotgun sequence.
ACCESSION   DBNHOH010000968 DBNHOH010000000
VERSION     DBNHOH010000968.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1215941"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..1620
                     /locus_tag="AC16V7_17520"
     CDS             286..1620
                     /locus_tag="AC16V7_17520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HIR6258979.1"
                     /translation="MEATKSKKSWADRLAESMEVMAPLFDQPHLASIRDGFVALMPLI
                     IIGAVVLIVLQFPCSLQEGSECYLGDTPFFTQAAEGQMSLADKLWVPFNITFGLLSVY
                     VTLSISYALARRRGLDPVMPLIFAFLSFLLMASPQVAGGEPATYLDNSGLFTAILVSV
                     VTIEVYRFFIQRNLVITMPAGVPPAIANAFAALVPGAVIIFGWWFIRFLLNIDIAEGL
                     FQVLSKVVPAASTYVAVAIAETFHGFLWTMGIHGDLTIGIALEPVWTANLAANAQAVA
                     AGQAPTAIYTSLFRSYVVPGGSGATLPLAFYFIRSKAPRLRRVGWLGIWPGIFNINEP
                     ITFGAPVIFNPVLAIPFIIITFLNATIAYLAHTLNLVTPTYIAAPWTLPSLILMFMAT
                     GFDWRASVLVIITEFVLPGIIWWPAFKAWERQVLEKEVVPSEAAPAAAPATA"
     gene            1691..>2695
                     /locus_tag="AC16V7_17525"
     CDS             1691..>2695
                     /locus_tag="AC16V7_17525"
                     /inference="COORDINATES: protein motif:HMM:NF014148.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 4 glycosyl hydrolase"
                     /protein_id="HIR6258980.1"
                     /translation="MKLTFIGAGGVRTPLVIQSILDYQERLPLTQVCLMDIDAERLDL
                     MRLASKSRLASGANFNVEWTTDARQAIEGSDFIVFTFRVGLMESRVIDEQVPLKYGVL
                     GQETTGPGGFAMAMRTIPVALDYVRLVRELAPDAWLLIFTNPAGMVTEAITRVAGFER
                     AVGICDNPAYMLRSAAAFLGVKSHQLFPEYYGLNHLGWLRAIYLDGVDQVPAIMRALK
                     SSKLDWHGLPFNSEMIEALGVIPNEYLYFFYYSRQSVENLLRAGQSRAQQILPFNQEL
                     YASLRRIRDEEDAPHLVDAAYARYLGKRHGTYMTLETGEKTSVSGEDAPVKSEAKTQI
                     A"
BASE COUNT          540 a          768 c          746 g          641 t
ORIGIN      
        1 cacgcgtcta cagaacatag cggcgggcgg tataacgctc ttggagactg atttccatga
       61 gcatgcagtc atcggcgtcg atgtgcagaa gttatcgtta atcagatcat gcttcctgcc
      121 atcatgcaaa aattgggctg gctgatcatc agcatataga gcggcgtgcc ccgattgacg
      181 gtttctaaat cccgaaggag gtggagagcg aaaagacttc aaccgtttca ttcatcccta
      241 atatcgaatc ctcttttata gaatcactgg aaaggagcct gtgaaatgga agcaacaaaa
      301 agcaaaaaaa gttgggcgga ccgtttggcc gaatccatgg aggtgatggc gcccttattt
      361 gatcaacccc acctggcctc catacgcgat gggttcgtgg ctttaatgcc gctcatcatc
      421 atcggcgccg tcgtgctgat cgtcctgcaa tttccgtgca gcctgcagga aggttcagaa
      481 tgctacctgg gtgacacgcc tttctttacc caggctgcag aagggcagat gagcctcgca
      541 gacaagctgt gggtgccttt taatatcacc ttcggtctct tgtctgtcta cgtaaccctg
      601 tctatctcct atgccttagc acgccgcagg ggactagatc cggtgatgcc tttgattttc
      661 gccttcctgt cgtttctgtt gatggccagc ccgcaggtgg cgggcggcga gccagcgacc
      721 tacctggata actcaggcct gttcaccgcc atcctagtct cggtggtgac gattgaagtg
      781 tatcgcttct tcattcagcg aaacctggtc atcaccatgc cggctggtgt gccaccagcc
      841 attgcaaatg ccttcgccgc cctcgttcca ggggctgtca tcatattcgg ctggtggttc
      901 atccgtttcc tgctgaacat cgatatcgcc gaagggctgt tccaggtctt gtccaaggtt
      961 gtgccagccg cgtcgacata tgtggccgtg gccatcgcag agaccttcca tggattcctg
     1021 tggacgatgg gcattcatgg tgacttgacc atcggcatcg cgctggagcc ggtatggaca
     1081 gccaatctgg cagccaatgc gcaggccgtc gctgctggcc aggcgccgac agcaatttac
     1141 accagtctat tccgctccta tgtggtgccc ggcggctcgg gcgctacatt gcccctggcg
     1201 ttctacttca tccgctctaa agcgccgcgc ctgcgccgcg taggctggct ggggatctgg
     1261 cctggtatct tcaatatcaa cgagccgatc accttcggcg caccggtgat cttcaacccg
     1321 gtgctggcga tcccctttat catcattacg ttcctcaacg ccaccatcgc ctacctggcg
     1381 catacactca acctggtgac gcccacttac atcgcagccc cgtggactct gccgtcactt
     1441 atcttgatgt ttatggccac cggcttcgat tggcgggcaa gcgtcttggt gatcatcacc
     1501 gaattcgtct tgcctggtat catctggtgg ccggctttca aagcctggga gcgccaggtt
     1561 ctggagaagg aagtggttcc ttcagaagca gcgcctgcag ccgcgccagc cacggcttga
     1621 attccgtaaa aacagccaat tttgggggga tgtgggcagc cgcatccccc ttgatgccta
     1681 gaagatattc atgaaattga ccttcatcgg tgccggtggt gtccgcacgc cactggtgat
     1741 ccagagcatt ctggattacc aggaacgcct gccgctcacc caggtctgcc tgatggatat
     1801 tgacgccgag aggctcgacc tgatgcgtct ggcgagcaag tccaggctgg ccagtggcgc
     1861 aaattttaat gtcgagtgga ccaccgatgc ccgccaggct attgaaggtt ccgatttcat
     1921 cgtgtttacc tttcgcgttg ggctgatgga atcgcgggtg atcgatgagc aagtgccgct
     1981 taagtacggt gtgctcggcc aggagaccac cggtccaggc ggcttcgcca tggccatgcg
     2041 aaccatcccg gtggcacttg attatgtgcg cttagtacgc gagctggcgc ctgacgcatg
     2101 gttgctcatt ttcaccaacc cggcagggat ggtcacagaa gccattacgc gagtggcagg
     2161 atttgagcgt gcggtgggca tctgcgacaa tccggcttac atgctgcgct ccgcggcggc
     2221 tttcctggga gtcaaatcgc atcagttgtt ccccgagtac tacggcctca accacctggg
     2281 ctggctgcgg gcaatctatc tcgatggggt ggaccaggtg ccggcaatca tgcgggcgct
     2341 caaatcctct aagcttgact ggcacggtct tcccttcaac agcgagatga tcgaagccct
     2401 gggcgttatc cctaacgagt atttatactt tttctattat tcgcggcagt cggtggaaaa
     2461 cctgctccgg gcggggcaaa gtcgagccca acaaatcctg ccttttaatc aagagctgta
     2521 tgccagcttg cgcagaatcc gagatgagga agatgctccc catctggtcg atgccgccta
     2581 tgcccgttat ctggggaagc gacacgggac ttatatgact ctcgagaccg gcgagaagac
     2641 ctctgtctct ggagaggatg ctcctgtcaa gtcggaagcc aaaacccaga tcgct
//
LOCUS       DBNHOH010000969         3041 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1600290, whole genome shotgun sequence.
ACCESSION   DBNHOH010000969 DBNHOH010000000
VERSION     DBNHOH010000969.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1600290"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1375)
                     /locus_tag="AC16V7_17530"
     CDS             complement(89..1375)
                     /locus_tag="AC16V7_17530"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019588037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR6258981.1"
                     /translation="MQHTIPVETLGAMGEHMANAVGKCVHCGFCLPACPTYNLLGEEM
                     DSPRGRIILMKSVLEGEVELEEALPYIDRCLGCLGCVSACPSGVPYGELVTPFRSFAQ
                     ERRRRPLTQRAARQLALDSLPSPERFRIAAKAGRAARPFQGVLPKQVRAMLDLLPGAL
                     PVSQPLPDLFPALGPRKARVALLTGCAQQVLAPQINWSTLRVLARNGVEVVIPQGQGC
                     CGALALHAGEISLARAQATYNLRLFPKDVDAVVTNAAGCGSGMRDYPLLFSLSQNGVD
                     DEDGWLDLVERFAHKVYDISAFLDDLGIEEPPPLPNQLKLAYHDACHLAHAQGIMEAP
                     RRLLRLIPNLTLVALPEGDLCCGSAGLYNIEQPETAQRLGERKAKHIANVEAQAVVTG
                     NIGCIVQMRTHLAQVGHSLPVWHTIEVLDRAYHGWG"
     gene            complement(1404..2519)
                     /locus_tag="AC16V7_17535"
     CDS             complement(1404..2519)
                     /locus_tag="AC16V7_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR6258982.1"
                     /translation="MIKESVKPTSVIEIQELIRQQPKLAARGGGSKSALHPASIDGLL
                     VDMTGLAGLLDYQPEEFTFTAWAGSRVDEIQPVLGERGQFLPFDPVLVERGATLGGTV
                     ASGLSGPGRYRYGGLRDFLLGIKFVDGMGNLVRSGGKVVKNAAGFDLSKFMVGSLGHY
                     GVLVEVTLKVFPQPAEYLTLRVEYPAVSGALAALTKLTVTPFELFALDLEVHEGGCAL
                     LVRLGGLPETLPARLDRLRSELLALPQVQGVNAEAFSRFEGSTEAELWRSAREFLWVK
                     PGHTLVKVPLTSQRVPPLDEWLADHGVLRRYSAGGNLAWIAWQGSIEPLHALLQEQGF
                     SGLAILGSVDQPFLGKRVGDAFARRVKNALDPQCKFG"
     gene            complement(2530..>3041)
                     /locus_tag="AC16V7_17540"
     CDS             complement(2530..>3041)
                     /locus_tag="AC16V7_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013015000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HIR6258983.1"
                     /translation="SDPYEVHEAKDDAERMRIWKGRKSAFSAVGRLSSDYIVQDGVVP
                     RSRLGEALAEIQRLSAQYNLRVANVFHAGDGNLHPLILYDGREAGAYQRAEQLAGEIL
                     RLCVRYGGSITGEHGVGIEKRDYLPVMFSEADIDALHAIRRQMDPAGIANRGKMFPLG
                     ETSSIVGDQ"
BASE COUNT          680 a          954 c          807 g          600 t
ORIGIN      
        1 gcggatttcc atcatctctt gaaccagtgg tttcaaggtc ttcagggctt cgttagcttg
       61 ttcaacagtg aagtagcgcg gcatcttttc aaccccatcc atgataggct cgatcgagca
      121 cttcgatggt gtgccagacc ggtaaagaat gtcccacttg cgctaaatgg gtgcgcattt
      181 gtacaatgca gccgatgttg cccgtgacaa ccgcttgggc ttcgacgttc gcgatgtgct
      241 ttgctttgcg ctcgcctaga cgctgagcag tctcgggttg ctcgatatta tataagcccg
      301 ccgacccaca gcacaagtca ccttcgggaa gagccaccag ggtcaggttg gggattagcc
      361 gcaatagccg gcgaggtgcc tccatgattc cctgggcatg cgccaggtgg caagcgtcat
      421 gataagccag tttgagctga ttcggcagtg gtggaggttc ttcgataccc aaatcatcca
      481 ggaaggcgct gatatcgtag accttatgag caaatcgctc gaccaaatcc aaccagccat
      541 cttcatcatc caccccattc tggctcaagc tgaacagtaa gggataatct ctcattcctg
      601 acccacaccc ggcggcgttg gttaccactg catcaacatc cttggggaag aggcgtagat
      661 tgtaggtggc ctgagcgcgc gccagggaaa tctcgccagc gtgcaaagcc agcgccccgc
      721 aacaaccttg cccctgaggg atcaccacct caacgccatt gcgcgccaga acacgcaggg
      781 tagaccagtt aatctgcggg gcaagcacct gttgggcaca gccggtcagc agcgccacgc
      841 gtgcttttct aggtccaagg gctggaaata aatctggtaa gggctggctg accggcaacg
      901 ccccggggag caaatccagc atagcgcgca cctgcttagg cagcaccccc tgaaacgggc
      961 gggcagccct gccagcttta gctgctatac ggaaccgctc aggggatggc aggctatcca
     1021 gagctagttg gcgggctgcg cgctgagtca aaggcctgcg ccggcgctcc tgggcaaagg
     1081 aacggaacgg cgtgaccagt tcgccataag gcactcccga ggggcaggcg ctgacacagc
     1141 ccaggcatcc caggcagcgg tctatgtatg gcaaggcctc ttccagctca acctccccct
     1201 ccaagacgga tttcatcaga ataatccgcc cgcgcggtga atccatctcc tcaccaagca
     1261 agttataggt ggggcaggct ggcaggcaaa aaccgcaatg cacacatttt cccactgcgt
     1321 tagccatgtg ctcgcccatg gcacctaaag tttcaaccgg tatagtgtgt tgcatactca
     1381 gcacatgtcc tgatattcat ggttcaccca aacttacact gggggtctaa ggcgtttttc
     1441 accctgcggg caaaagcatc tccaaccctc ttcccgagaa aaggctggtc gactgaacct
     1501 aaaatcgcca ggccagagaa cccctgctct tgcagtaatg catgcaacgg ctcgatcgag
     1561 ccctgccagg ctatccaggc caggttgccc cccgcgctgt agcggcgtaa tacgccgtga
     1621 tctgcaagcc actcatccaa gggcggaacg cgttgcgagg tgagtggcac tttcaccagg
     1681 gtgtgtcccg gcttcaccca taaaaattca cgcgccgagc gccatagttc agcctcggta
     1741 ctgccctcaa acctggaaaa tgcttcggca ttaacacctt gcacttgcgg tagcgcaagc
     1801 agctcactgc gcaggcgatc caaacgtgca ggcagagtct ccggcagccc gccgagacgc
     1861 acaagcaacg cacaaccccc ctcgtgcact tccaggtcaa gcgcgaaaag ctcgaagggc
     1921 gtgacggtta atttggtgag agcagccaga gcaccagaca cagcagggta ttcaacccgc
     1981 agtgttaaat attcagccgg ctgagggaac accttcaggg ttacctccac caagacacca
     2041 taatgcccca ggctgcccac catgaacttg gataggtcga aaccagccgc gttcttcacc
     2101 acttttcccc cgctgcgcac caggttgccc atgccatcca caaattttat gcccagcaga
     2161 aaatcacgca gcccgccgta gcggtagcga ccaggaccgc tcaaaccaga ggcgaccgtc
     2221 ccccccaggg tagcgcctcg ctcgaccagc accgggtcga agggcaggaa ctggccgcgc
     2281 tcacccagca ctggctggat ctcatccacg cggcttccag cccaggcggt gaaggtaaat
     2341 tcttcgggct ggtaatccag caaacctgcc aggccggtca tatccactaa aaggccatct
     2401 attgaggcag gatgcagggc agacttgcta cccccgccac gcgcagccag tttcggctgt
     2461 tggcggataa gctcctgtat ctcgatgacg ctggtgggtt ttacgctttc cttgatcatt
     2521 gttaacagct tactgatcgc ccactatgga tgatgtctca cccaagggga acattttccc
     2581 acggttcgca atccctgcag gatccatctg ccgccggatg gcgtgcaagg catctatatc
     2641 ggcttcgctg aacatcacgg ggaggtaatc ccgcttctcg atgcccacac cgtgctcgcc
     2701 agtgatcgag cctccatacc gcacgcatag ccgcaatatc tcacccgcaa gctgctcggc
     2761 gcgctggtat gcacccgcct ccctgccgtc gtacaagatc aatggatgca ggttaccatc
     2821 gccggcatgg aagacgttcg ccacccgcaa gttgtactgg gcactcaggc gttgtatctc
     2881 cgccaaggcc tctcccaagc ggctgcgcgg cacgacacca tcctggacta tgtaatcaga
     2941 actgagccgg cccacagcgg aaaaggcact tttgcgccct ttccagatgc gcatccgctc
     3001 ggcatcatct ttcgcctcat ggacttcgta gggatccgag g
//
LOCUS       DBNHOH010000970         6837 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_895737, whole genome shotgun sequence.
ACCESSION   DBNHOH010000970 DBNHOH010000000
VERSION     DBNHOH010000970.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6837)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6837)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6837
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_895737"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="AC16V7_17545"
     CDS             complement(<1..343)
                     /locus_tag="AC16V7_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HIR6258984.1"
                     /translation="MTIVIDGSDLTVEKMVRIARDGERVELHPEALDRIKACRGMLEE
                     KLKAKEIMYGTNTGIGEFSEVVLTDEQVEQFQRYLIYNHAAGIGEPVSIENVRGAIAS
                     RINVHAHGMSGC"
     gene            643..1044
                     /locus_tag="AC16V7_17550"
     CDS             643..1044
                     /locus_tag="AC16V7_17550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258985.1"
                     /translation="MSLTLQRLNPNLPTTYWLIWLGSFTVPSLSLYLTNQRDISISQA
                     ALMVSLFGIASFTASLAGGELADRLSFHGGDKFHRDLAKDGVCRNSPGGEISRAFPEM
                     RRIHSLQRSPHSIRCAAGECRCHVQSISTIR"
     gene            1060..1713
                     /locus_tag="AC16V7_17555"
     CDS             1060..1713
                     /locus_tag="AC16V7_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013402226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HIR6258986.1"
                     /translation="MEEKMQLLTDLHLAIIDGQRDQVVDLVKRLLEQHADPEIILRNS
                     MIPAMDIVGKRFEEELFFVPEMLIAARAMQAGLDILQPSLAASNIEPIGNVVIGTVQG
                     DIHDIGKNLVAMMLGGAGFRVVDLGVNVPAQKFVDAVQNENGVQIVSLSALLTTTMVN
                     MSAVLEALDKAGLRPDIKVMVGGAPITEAYAQKIGADGYAPDASQAVGLAKRLLGIV"
     gene            1781..3034
                     /locus_tag="AC16V7_17560"
     CDS             1781..3034
                     /locus_tag="AC16V7_17560"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HIR6258987.1"
                     /translation="MPSVQTTAVHASSPDMNRIFQRLNLNLPPTYWLIWLGTLINRLG
                     SFVIPFLTLYLTNQRGISISQAALMVSVFGIGSFIASLTGGELADRLGRRPVMLISFL
                     IAPLNMVLLGLARPVALIATLTLLQGFFTDLYRPAVNAVVADLVEPEGRPRAYGYIYW
                     AINLGAALAPLIAGLMARLDYFLLFLGDAITTFIFGLIVLWGVRETRPAEAQITTRTA
                     VRDRITKLGQEPLLLLFAGLALVFGMIYMQGYVTLPVDMQANGMRPDQYGLVISVNGA
                     LIVLLSIPVSNSAVRWPRFTALAAAALLMGMGYGLTAISSTMLLYAISVAIWTLGEIA
                     GAAVAPSVVADLSPVELRGLYQGVFGAAWGLSFFVGPMIGGWVLEHLGGWALWGGCFA
                     LGCLLALGYLAMTRPAQRRMARTSG"
     gene            3131..3577
                     /locus_tag="AC16V7_17565"
     CDS             3131..3577
                     /locus_tag="AC16V7_17565"
                     /inference="COORDINATES: protein motif:HMM:NF020977.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HIR6258988.1"
                     /translation="MSVKSELENALKDAMRAGNDLHKRTLRMALSAIKQTEIDKGITL
                     DDSGVMAILQKEVKTRREAIADAERANRPDLTAASQAEITFLESFLPKELTPEELEDL
                     ARQAIAETNASSIREMGQVMKVLMPRLQGRATGDVASQTVRRLLAG"
     gene            3588..5747
                     /locus_tag="AC16V7_17570"
     CDS             3588..5747
                     /locus_tag="AC16V7_17570"
                     /EC_number="3.1.4.59"
                     /inference="COORDINATES: protein motif:HMM:NF019317.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0106409 - cyclic-di-AMP phosphodiesterase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD family phosphohydrolase"
                     /protein_id="HIR6258989.1"
                     /translation="MKVNGYRIVLLVVAVALSFIALVAPQLIPRSSQSLQAGQVAIQD
                     ILAPRDISFESEILTGQQREAVIRAVAPVYTPADTSISRRQLEKLRNALAFITSLRAD
                     PYASIEQKLTDLAALEDIQLSQETALRILALNDSRWQVIQQETIVVLEQVMRNTIRED
                     RLDDARRSVSSLVSLSLPEDQADIVTRLASALVAANSFYSEALTENARQEALDGISPI
                     IREYKAGETVVQRGQIISETDLEALGQLGLIQTQFNWKELVSAAAIVFLGIGFNLLYL
                     RYNTKIREDARGLAVITVLFLVFLLSARLIISGHVVLPYVFPLAAYGLTVAVLFGAEP
                     ALVSSLSLAILVAYGYPNALDLTLYYALSSFFGVLSLGRGQRILTFFWAGAVIASSGS
                     LVAIAFRLPLPETDWVGITTLVGAALLNGTASASLAVLLQFFLAQLLGMTTALQLLEI
                     SRPDHPLLQFALRNAPGTYQHSLQVANLAEQAAEHIGADALLTRVGALYHDVGKALNP
                     AFFIENQMPGNPNPHDQLDPLPSASIITKHIKDSLELARKYRIPRRLQDFMLEHHGTM
                     IARYQYVNAVKAAGGDESKVDPELFRYPGPRPRSRETAILMLADGCEARVRAERPKDT
                     DEMRALIKSVIQNRMSQGQLDETDLTLRDLDAIAESFITTLRGVYHPRIEYPNLEKTT
                     PSATEAVPTLPTSAQTAQEANPSPKSNPPTPAPDSSS"
     gene            5744..6211
                     /gene="ybeY"
                     /locus_tag="AC16V7_17575"
     CDS             5744..6211
                     /gene="ybeY"
                     /locus_tag="AC16V7_17575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR6258990.1"
                     /translation="MIHLQVSESLAKSIPIAPEILSDLERAGMQTLQQAGIQGSDLTI
                     VITDDAELHELNHRFLGIDTPTDVLSFPTDETDPDSGVRYLGDILISYPRAVAQADAG
                     RHPIQDELRLLVVHGVLHLLGHDHAEPDERDRMWELQGEVLKQLGSSVTLPKE"
     gene            6236..6628
                     /locus_tag="AC16V7_17580"
     CDS             6236..6628
                     /locus_tag="AC16V7_17580"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HIR6258991.1"
                     /translation="MFSFLSSRARAFRYAFRGWSFVIRTQRNAWIHAVISVAVIIISA
                     WLHLSPRDWAGIIIAIAMVWTAEFINTALEAVVDLASHHEHHELARVGKDVGAAAVLI
                     AAMAAILIGFLILGPPLWERLRPLVAGP"
BASE COUNT         1401 a         2045 c         1855 g         1536 t
ORIGIN      
        1 ggcagcccga catgccgtgg gcgtgcacgt taatgcggct ggcgatagca ccgcgcacgt
       61 tttcaatgga gactggctcg ccgatacccg cggcgtggtt gtagatcagg tatcgctgga
      121 actgctctac ctgctcgtcg gtgagcacca cctcggagaa ctcaccaatg ccggtgttcg
      181 tgccgtacat gatctccttc gccttgagtt tctcctccag catgccacgg caggctttaa
      241 tacgatccag cgcttcaggg tgcaactcca ctcgctcgcc atctctggcg atgcgtacca
      301 tcttctcaac tgtcagatcg gaaccgtcta taacgatcgt catgtggcca ttctccttga
      361 tctgagttct gattactaaa aacggcgagg cagggtttct tggcatcaaa gtcagacggg
      421 gaaaacccga tgatctttga ccgcttctgc ttccgcactt cctgagaggt tcgggcgttt
      481 gtctttataa ttatatcccc gaaggtaggc ttataacctg tgtgaggtgc attcagttac
      541 aatttaatga agacgtgaag ctggaggagt tcggagaagc tcgatatcaa tttattaatt
      601 tcccaacaca tttcctgtaa tatacttagt gctgtgtctt ccatgagcct aacacttcag
      661 cgcctcaacc ccaacctgcc gacaacatac tggctcatct ggctgggcag cttcaccgtc
      721 ccctccctgt cactttacct gaccaaccag cgggatatct cgatctcaca ggcggcgctg
      781 atggtatcgc tgtttggcat cgcgtcattt acagcatcct tggcaggcgg tgagttggcc
      841 gaccgtctga gtttccacgg tggggataaa tttcatcgag accttgccaa agatggcgtc
      901 tgccgaaata gcccaggagg tgaaatatcg cgtgcgtttc ctgaaatgcg gcggatacat
      961 tctctccagc gctcaccaca ttcaatccga tgcgccgctg gagaatgtcg ttgccatgta
     1021 caatccatat ctacgatacg ctgagggttg ataaaaatta tggaagagaa aatgcaattg
     1081 cttaccgatc tgcacctggc aataatcgac gggcaacgcg atcaggtggt ggatctggtg
     1141 aaaaggcttt tggaacagca cgccgacccg gaaatcatat tgaggaattc gatgataccg
     1201 gcaatggaca tcgtgggcaa gcgttttgaa gaggagcttt tctttgtacc tgaaatgctg
     1261 atcgccgcac gagcaatgca agccggcctg gacatcctcc agccgtcgtt ggctgcgtcg
     1321 aatatcgaac cgattggaaa tgtcgtaata ggaaccgtgc agggcgatat ccacgatatc
     1381 ggcaagaatc tagtcgccat gatgctcggg ggtgccggtt ttagggtggt ggaccttggc
     1441 gtaaatgtac ccgcgcagaa gtttgtggat gcagtgcaaa acgagaacgg ggtgcaaatc
     1501 gttagtttgt cggcattgct tactacgaca atggtcaaca tgtcggcggt gttggaagcg
     1561 ctggataaag ctggcctgcg cccggatatt aaggtgatgg taggcggtgc cccgattacg
     1621 gaagcttacg cccaaaagat cggtgccgac ggctatgccc cagacgccag ccaggctgtc
     1681 gggttggcca agcgcctact aggcatcgtt taagctccgc tcttatcctg agagtccttg
     1741 acccaagaaa agcgcttttg tcttgaaaga tgaaggccgg ttgccttctg tccaaacaac
     1801 cgctgttcac gcctcaagcc cggacatgaa ccgaatattt cagcgcctca acctcaacct
     1861 gccgcctacc tactggctca tctggctggg tacgctcatc aaccggctgg gcagcttcgt
     1921 catccccttc ctgacacttt acctgaccaa ccagcggggt atctcgatca gccaggcggc
     1981 gctgatggtc tctgtgtttg gtatcggatc atttatcgct tcgctgacag gcggcgagct
     2041 ggccgaccgc ctgggccgcc ggccggtcat gctgatcagc ttcttgatcg ctccgctcaa
     2101 catggttctc cttggactgg cgcgcccggt tgcgctgatc gccaccctca ccttgctcca
     2161 gggcttcttc accgatctat atcgcccagc ggtcaacgcc gtggtagcag acctagtaga
     2221 gccagaaggg aggccgcgcg cctacggcta tatctattgg gcgatcaacc tgggcgccgc
     2281 acttgcacca ctcatagcag gcctgatggc gcgcctggat tactttttgc tgtttcttgg
     2341 cgatgcaatc accaccttca tttttggcct gattgtgttg tggggcgtgc gtgagacgcg
     2401 gcctgccgag gcgcaaatta ccacccgaac cgcggtgcga gatcgcatca ccaagctcgg
     2461 gcaagagcct cttttgttgc tcttcgccgg gttagccctg gtctttggaa tgatctacat
     2521 gcaagggtat gtcacgttgc cggtggacat gcaagccaat gggatgcgcc cagatcaata
     2581 cggattagtg atctctgtca atggcgcgct gatcgtgctg cttagcatac cggtgagcaa
     2641 ctcggcggtg cgctggccgc gctttactgc gctggctgcg gcggctttgc tcatgggcat
     2701 gggatacggc ctgacggcca tctcgtcaac catgttgctg tacgccatct cagttgcaat
     2761 ctggaccctg ggcgagattg ctggcgctgc ggtagccccc tcggtggtgg cagacctatc
     2821 cccggtagag ctgcgcggct tgtaccaggg cgtcttcggc gccgcctggg ggctttcgtt
     2881 ctttgtcggc cctatgatcg gcggctgggt gttggagcat ctcggaggct gggccctttg
     2941 ggggggatgc ttcgcattgg ggtgcctgct ggctctggga tacctggcga tgacacgccc
     3001 cgcccaacgg cgcatggcgc gtacttcagg ctaaaatgtc agcgtcgtca tggggcgtgt
     3061 ataagcggag cacctttctc acctccactg cccatgctgt tgaatatgaa atattaggta
     3121 taatgatctc atgagcgtca aatccgaact cgaaaatgcc ctcaaagacg ccatgcgcgc
     3181 tggcaatgac ctgcacaagc gcaccctgcg catggcgctc tctgctatta agcaaaccga
     3241 gatcgataaa ggcattaccc tggacgactc gggggtgatg gcgatcctgc agaaagaggt
     3301 caaaacgcgg cgcgaagcca ttgcagacgc cgagcgcgcc aaccgtcccg acctgaccgc
     3361 cgcatctcag gcagagatca ccttcttaga aagctttctc cccaaagaac tcactccaga
     3421 ggaactggaa gatctggcac ggcaggctat cgccgaaacg aacgctagct caatccgtga
     3481 gatgggtcag gtgatgaaag tgctcatgcc acgcctgcag ggtcgggcaa ctggagacgt
     3541 agccagccaa acggtgcgca ggctgctagc aggttaaggg tttcatcttg aaagtaaatg
     3601 gctacaggat tgtattgctg gtggttgccg ttgcgctctc gttcatcgcg ctggtagccc
     3661 cccaactcat tccccgctct tcgcaatccc tgcaagctgg acaggttgct atccaggata
     3721 tcctggcccc gcgcgatatc tctttcgaga gcgaaatcct taccgggcag cagcgcgagg
     3781 ccgttattcg ggctgttgcc ccagtctata ccccagccga caccagcatc tcccgccggc
     3841 agctggagaa acttcgcaat gccttggcct tcatcaccag tctacgcgcg gacccctacg
     3901 cctccataga acaaaaatta accgatctag ccgcgctgga agacatccag ctcagccagg
     3961 agactgctct gcgcatcctg gccctcaacg attcgcgctg gcaggtcatc cagcaagaga
     4021 ccatcgtagt cttggaacaa gtgatgcgta acacgatccg ggaggatcgc ctggacgatg
     4081 cccggcgcag cgtctcttcc ctggtcagtt tatctcttcc tgaagaccag gcggacattg
     4141 tcacccgact cgcctcagct ttggtggcgg ccaacagctt ctacagtgaa gcccttaccg
     4201 agaacgcccg ccaagaagcc ctggatggga tcagcccgat catacgggaa tacaaagccg
     4261 gcgagacggt ggtgcagcgc ggccagatca tctccgagac cgatctggaa gccctgggac
     4321 agctggggct aatccagacg caattcaact ggaaagagtt ggtcagcgcg gcggccatcg
     4381 ttttcctggg gattggtttt aacctgctct acctgcgcta caacaccaaa atacgggaag
     4441 atgcccgtgg cctggcagtc atcaccgtac tgttcctggt cttcctgctg agcgcgcgcc
     4501 tgatcatctc ggggcacgtt gtcctgcctt acgtctttcc attggcagct tacggcctga
     4561 cggtggctgt gctattcggc gcagagcctg ccctggtctc gtcgctatcc ctggcgatac
     4621 tggttgcata tggttatcca aacgccctgg atttgaccct atattatgca ttgagcagtt
     4681 tctttggggt gctcagcctg ggacgaggcc agcgaatcct gaccttcttc tgggcaggcg
     4741 cggtaatcgc cagtagcggg agcctggtcg ccattgcctt ccgattaccc ctgcccgaaa
     4801 ccgattgggt cggcatcacc accttggttg gtgcagcgct gctgaatggc accgcctcgg
     4861 ccagcctggc tgtgctgctg caattcttcc tggcccaatt attgggcatg accactgctt
     4921 tgcaactcct ggagatttca cgtccagacc accccttgct gcaatttgcg ctgcgcaatg
     4981 cgcctggtac ctaccagcac agcctgcagg tggcaaacct ggccgaacag gcagccgaac
     5041 acatcggcgc tgacgctctg ctaacccggg tgggcgcact gtaccacgat gtgggcaaag
     5101 ccctcaaccc ggccttcttc atcgaaaacc agatgcccgg caatcccaac cctcatgatc
     5161 aactagatcc gttacccagt gcatcgatca tcaccaaaca catcaaagat agcctggagc
     5221 tggcgcgcaa gtatcgcatc cctcgccgcc tccaggattt catgctggag catcatggca
     5281 ccatgatcgc ccgttaccag tacgtgaacg ccgtgaaggc cgctgggggt gacgaaagca
     5341 aggttgatcc ggagctcttc cgctatccag gtccacgccc cagatcgcgt gagaccgcca
     5401 tcctgatgct tgccgacggc tgtgaagccc gggtgcgcgc cgagcgcccc aaagacaccg
     5461 acgagatgcg cgccctgatc aagagtgtga tccaaaatcg catgtcccag ggacagctag
     5521 atgagaccga cctaaccttg cgtgacctgg atgcgattgc cgaatcattc atcaccacgc
     5581 tacggggtgt ttatcacccg cgcatcgaat accccaactt ggaaaaaacc accccttccg
     5641 caaccgaggc ggttcccacg ttgcccacca gcgctcaaac tgcgcaggaa gcaaatcctt
     5701 ctccaaaatc taatccacca actcctgccc ccgattcatc atcatgattc acctgcaggt
     5761 ctcggaatct ctcgccaaat ccatcccgat agcacctgaa atcctgtctg atctggaacg
     5821 cgctggcatg cagaccctgc aacaggcggg catccaaggc tcagatttga ccatcgtcat
     5881 cacagacgac gctgaacttc atgagctcaa ccaccgcttc cttggaatcg acacccccac
     5941 ggatgtgctc tccttcccca cagacgaaac tgaccccgac tctggcgtgc gttatctagg
     6001 cgacatcctc atatcctacc cgcgggctgt cgcccaggca gatgcggggc gtcatccaat
     6061 ccaggatgaa ttgcgtctcc tggtcgtgca tggggtgcta cacttgctgg gccatgacca
     6121 tgccgagccg gatgagcgtg accgcatgtg ggagctacag ggggaggtct tgaagcaact
     6181 gggcagcagc gtcacgctgc ccaaggaatg atcatcgatc cagcaaaaca cccttatgtt
     6241 ctctttcctg tcatctcggg cccgcgcctt tcgctacgct tttcgcggct ggtcgttcgt
     6301 catccgcacc cagcgcaacg cctggattca tgccgtgatc agcgtggcgg tcatcatcat
     6361 ctcggcctgg cttcacttat cgccccgtga ttgggctgga attatcatcg ccatcgccat
     6421 ggtgtggacc gctgaattca tcaacacggc gctggaagct gtggtagatc tggccagcca
     6481 tcacgagcac cacgaactgg ctcgcgtggg caaagatgtc ggcgccgcag ccgtgctgat
     6541 tgctgccatg gctgccatac tgatcggttt tctcatcttg ggtcccccct tgtgggagcg
     6601 gctgagaccc ctcgtggctg gaccctgatc tcccgcccat acttattgaa tcatcgaacc
     6661 tggagctatc tatgtcttct acgtcatttc gctttggcac tgtcggttca cccatctcca
     6721 ccccctccaa gcccggtggc agtgtgggtg ctgtgttgcg ttccgccgag ctagacctat
     6781 ttgccctgga actaggctgg gtgcgcgccg tgcgcgtctc ccctgagacc tgcgcgg
//
LOCUS       DBNHOH010000971         3980 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_189598, whole genome shotgun sequence.
ACCESSION   DBNHOH010000971 DBNHOH010000000
VERSION     DBNHOH010000971.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_189598"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="AC16V7_17585"
     CDS             complement(<1..220)
                     /locus_tag="AC16V7_17585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6258992.1"
                     /translation="MTSKADVIRRWHKDSWANPPSSIGEAATKYFSDDYQGLDKDGNV
                     MSDKAAYIGMSQLLLTAFIGFKGVVHDIS"
     gene            complement(663..1451)
                     /locus_tag="AC16V7_17590"
     CDS             complement(663..1451)
                     /locus_tag="AC16V7_17590"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR6258993.1"
                     /translation="MSAVILQDEIVHYEVLGRGRPVFFLHDWIGSWRYWIPSMQATSL
                     SFRAYALDLWGFGDTSKNPSYYSIEQQVGLLEEFVNNLGIGKIALVGHGLGAVIAMLY
                     ASRHPEGVDRVMTIGMPFGEDRINSRLRLNSPSELASWLLGRSPAAEAAYVDAPKADH
                     KAILLSLTSLQGVELACLPHQLATACLLVHGGNDPLIETPNSNHVEELPEESQFIVFE
                     KSGHFPMLDEPGQFNRLLVDFLSLESGQSPRQLQLKEEWKRRVR"
     gene            complement(1481..1858)
                     /locus_tag="AC16V7_17595"
     CDS             complement(1481..1858)
                     /locus_tag="AC16V7_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR6258994.1"
                     /translation="MARILIAEDEKDIRELITFTLSFAGHQVTATTNGEEAYLTAQEM
                     LPDLILLDVRMPRMTGFEACEKIKANEKTRQIPVVFLSAKGQEVEIQTGLNLGAVEYI
                     VKPFAPEQLAERVGAVLANFGNG"
     gene            complement(1928..>3980)
                     /locus_tag="AC16V7_17600"
     CDS             complement(1928..>3980)
                     /locus_tag="AC16V7_17600"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR6258995.1"
                     /translation="DVLPCALPDVPLFERLRQTQGVFNAGDIGVESELAPLSEFLEAR
                     QARSLMALTLATGKDTQGILLAYADRESRFTPDEVGLARTIANQVSIAIQNARLYAET
                     RSLSQDLEQRVIERTAQLEKAHQRAETLLRINMELSTSLDLEQVLNQSLHLVNQIVDA
                     GQVSVLITRSGEKKFYRLASRGYAGSPDSDGQTTLINVDEGLAGWVIKARQTVLVDDV
                     LQDPRWLKLMDVPEPHHRSALAVPLLRGAECVGVLLLYHQDVGHFSDDHLDLIQAVAN
                     QVAVAVNNAELYRLIRDQAEDLGQMFRRQQVEASRARAILEAVADGVLVTDAKRNITL
                     FNAPAEEILGLDRSQVIGHSLDHFTGLFGRAAQRWNETIRTWTQDPSTYEQGELYAEQ
                     IDLDDGRVVSVHLSPVIMEDNFLGTVSTFRDITHQVQLDRLKSEFVATVSHELRTPMT
                     SIKGYVDVLLMGAAGSLTEQQVEFLDIVRDNTERLTVLVNDLLDISRIESGQVTLSML
                     PLNLDEIITQGAAALRTRAESDQKPMDIQFAPASELPLAMGDPERVRLILDNLLENAY
                     YYTAPKGRITVQVHATGDEIQIDVQDTGIGIPEEAQARVFERFFRGEHPFVLSTSGTG
                     LGLSIVQHLVEMHNGRIWVESSGVPGEGSTFSFTLPAFQHDQAGSQKFHDEQVRQLAK
                     E"
BASE COUNT          933 a         1083 c         1004 g          960 t
ORIGIN      
        1 cggaaatatc gtggaccacc cccttaaaac cgataaaagc agtcagtaaa agttggctca
       61 tgccgatata tgctgctttg tccgacataa cgtttccgtc tttgtcgaga ccttgatagt
      121 cgtcagagaa atacttcgta gccgcttccc cgatgcttga gggcggattg gcccatgaat
      181 ccttatgcca ccttcgtata acgtctgctt tactcgtcat cttgctctcc tttgggtatg
      241 attgggcgca gtttccgatg cgcacatttg gttggcaagg ttggatgaac caggaattga
      301 tgtaatgagc agtgatacga catccgctat tgccgtgggt ataccccata ttaactattg
      361 ctaaattaat ctgggacttt gcagatgcat cgttgaggtc ccggtttatg cccccattga
      421 caatcgctaa cttgtcaatt atttccaaag accgtggcta caacaaaaat attatagcgc
      481 atattgcgat aatttagaga tgttttggat tattaactaa tggttctcta atttttggag
      541 tcggacttaa ggaatttacg tgttgatcac ttcgtgtagg gaaaatcttc gtctgcgctc
      601 tgtgtgtctc agcgggttgg actgattggg ctggtggact ggcgtccacc actacgagca
      661 gattacctta cccttctttt ccactcttcc ttgagctgca gctgtcgggg actctgcccc
      721 gattcaaggc ttaagaaatc caccagaagg cggttaaatt gacctggctc gtccagcatg
      781 gggaagtgcc ccgacttctc gaatacgata aattgagatt cttcaggcag ctcttctacg
      841 tgattagagt tgggcgtttc gatgagggga tcattcccgc cgtgaactag caggcaagca
      901 gtggcaagct ggtggggcag gcaggccaat tccacccctt gcaggctggt cagagaaagc
      961 agaatggctt tatggtccgc cttgggagca tccacatagg ctgcctcagc agccggtgag
     1021 cgccccaaaa gccagctggc cagctcgctc ggggagttta ggcgcaggcg cgagttaatt
     1081 ctatcctcac cgaatggcat gccgatggtc atgacacggt ctaccccttc ggggtggcgg
     1141 ctggcataca gcatggcgat gactgccccc aacccatgac ccaccagggc aatcttgcca
     1201 atgcccaggt tgttgacaaa ttcttccaat aaaccgacct gttgttcaat ggaataatag
     1261 ctgggattct tgcttgtatc tccgaaaccc cacaaatcca gtgcataggc acgaaatgat
     1321 aaggaagtcg cctgcatgct tgggatccag tagcgccagg agccgatcca atcgtgcagg
     1381 aagaagaccg ggcgtccgcg gcctagcact tcgtaatgca caatttcgtc ctgcaggatg
     1441 acagcactca tgagtcagta gcacctgatc ttcagatcat tcaaccatta ccaaaattcg
     1501 caagaacagc tcccacacgc tcagccaatt gttcgggggc aaagggcttg acgatgtact
     1561 caaccgcgcc caggttcaac ccggtctgaa tctctacctc ttgacctttc gccgagagaa
     1621 acacaaccgg aatttggcgt gtcttctcat tggcctttat cttttcgcaa gcttcaaacc
     1681 cagtcatgcg cggcatacgt acatccagaa gtatcaggtc tggaagcatt tcctgtgccg
     1741 tcaagtaggc ctcttccccg ttggtcgtcg ccgtaacctg atgcccagcg aaactcagcg
     1801 tgaaagtgat taattctcgg atatcttttt cgtcttcggc aattaatatg cgcgccatgt
     1861 ttttatgatc catgccaaac gatactggat aagagagcag agaaatttca tcagattcgc
     1921 taccaggtta ctcctttgct aactgtctga cctgctcatc gtggaacttt tgactccctg
     1981 cttggtcatg ctgaaacgct ggcaatgtga atgaaaatgt acttccttcc ccaggaacgc
     2041 cgctgctttc gacccagatg cgcccattgt gcatctcaac caggtgctgg acgatggaga
     2101 gccctaaacc agtccctgag gtggacagga caaaggggtg ctcaccgcgg aaaaagcgct
     2161 cgaagacgcg agcttgcgct tcttcaggga tgccaatgcc tgtatcttgt acatctattt
     2221 gaatttcatc cccagttgca tgaacttgaa cggttatgcg accctttggt gctgtgtaat
     2281 aatatgcatt ctccagcagg ttatccagga tcaggcgcac gcgttctggg tcacccatag
     2341 caagcggcaa ttccgaagcg ggggcgaact gaatatccat cggcttttga tcgctctcag
     2401 cgcgggtccg taaggcagct gccccttgtg taatgatctc atccaggtta agcggcagca
     2461 tggagagagt tacctgaccg gattcgatac gggatatatc caaaagatca ttgaccagga
     2521 cggtcaggcg ttccgtgtta tcgcgcacaa tatctaagaa ctcaacttgc tgctcggtga
     2581 gtgacccagc tgcgcccatc agcaagacat cgacatagcc tttgatagaa gtcatggggg
     2641 tgcgcaattc atgactcact gtggctacga actcgctctt tagccgatct agctgcacct
     2701 ggtgggtgat gtctctgaac gtggagactg tacccaagaa gttatcctcc atgataaccg
     2761 gcgagaggtg caccgacacc acccgcccgt catccaaatc gatctgctcg gcgtagagtt
     2821 caccctgctc ataggtgctg ggatcctgtg tccaggtccg aatagtctca ttccagcgct
     2881 gagcggctct cccgaacaag ccggtgaagt ggtcgagcga atgaccaatc acctgtgatc
     2941 gatcaagccc aaggatctcc tctgcaggcg cattgaacag ggtgatattt cgtttggcat
     3001 ctgtaaccaa aacgccgtcg gctacagctt ccaggatcgc acgcgcacgg ctagcctcca
     3061 cttgctggcg acggaacatt tgccccaaat cttctgcctg gtcgcggatc aagcgataca
     3121 actcggcatt attaaccgcc accgctacct ggttggcgac agcctggatc aaatccaggt
     3181 ggtcgtcgga gaaatgtcct acatcttggt ggtacaacag caagaccccc acacactccg
     3241 ctcccctcag cagcggaaca gccaaggcac tacggtgatg aggctcgggt acatccatca
     3301 gctttagcca acgcggatct tgcaacacat cgtcaaccaa tactgtttgg cgagctttga
     3361 tcacccaacc agctagaccc tcatcaacat tgatgagggt ggtctgccca tcgctgtcgg
     3421 gcgagccagc atagccccgg gaagccaagc ggtaaaactt cttctcgccc gagcgggtga
     3481 tcaacacaga cacctgccct gcgtccacaa tctggttgac caggtgtagg gattgattga
     3541 gcacctgctc taaatctaga ctggtactca attccatgtt gatgcgcagc aaggtctcgg
     3601 cacgctgatg agctttctcc agctgggcag tgcgctctat gacacgctgc tccagatctt
     3661 ggctcaagct gcgggtctcg gcatacagac gagcattttg aatggctatc gaaacctggt
     3721 tggcaatggt gcgtgccagc ccaacctcat ctggggtaaa gcgagattcc cgatcggcgt
     3781 atgccagcaa gataccctgg gtatctttac ccgtcgccag ggtcaatgcc atgagggaac
     3841 gcgcctgccg ggcctcaaga aattcggata atggcgccaa ttcgctctcc acgccgatat
     3901 ccccggcatt gaacacaccc tgtgtctggc gcaggcgctc gaataacggg acatctggta
     3961 aggcgcacgg caatacgtcc
//
LOCUS       DBNHOH010000972         6054 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_639566, whole genome shotgun sequence.
ACCESSION   DBNHOH010000972 DBNHOH010000000
VERSION     DBNHOH010000972.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6054)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6054
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_639566"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /gene="truA"
                     /locus_tag="AC16V7_17605"
     CDS             complement(<1..649)
                     /gene="truA"
                     /locus_tag="AC16V7_17605"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR6258996.1"
                     /translation="MARYQVILAYDGTQYHGFQRQAKAHTVQGVIEATLRELGWSGRA
                     ILAAGRTDTGVHASGQVIAFDLDWKHSPDELGAALNAQLPDDVAARSVMQVEVDFHPR
                     YDALARTYRYRIYCWENRDPLLDRYAWRVWPALDLDLLQQAASLLVGRQDFAAFGTPP
                     RPSGSTQRTVFQAQWTRYDQELIFIITADAFLYRMVRRLVSFQVEIGQGRRELEAL"
     gene            complement(799..1149)
                     /gene="rplQ"
                     /locus_tag="AC16V7_17610"
     CDS             complement(799..1149)
                     /gene="rplQ"
                     /locus_tag="AC16V7_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011369741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HIR6258997.1"
                     /translation="MSRGKDERTALRRILIKQLFEHERIRTTRAKARAVRGQAERLIT
                     LAKRGNQAGEEKAVHARRLAAARLGDPVAVKKLFDEIAPRFESRPGGYTRILSLGPRL
                     GDAADMVILELIED"
     gene            complement(1181..2134)
                     /locus_tag="AC16V7_17615"
     CDS             complement(1181..2134)
                     /locus_tag="AC16V7_17615"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIR6258998.1"
                     /translation="MVTPKIERKAEARNYGKFIISPLERGYGVTLGNALRRVLLSSLD
                     GAAVTSIRFADVQHEFSDVPGVREDVIRIMLQIKQLRMKLSDVESARMHLEVRGAGVV
                     TAADIITPPEVEIINPDLYLFTVDDNNANLEIEMTVERGRGYSPADDRTGRLPIGELP
                     VDAIYSPVRRVNWDVGLARVGQSTNYDSLVMEIWTDGTIGPEESLSTGAKTLIDHLRH
                     LAGVSEESLASLAEAQEEAGRLTSEIIETPIENLDLSVRVFNSLKRTGITTIGEVIEL
                     LEKGDEAVMSIRNFGEKSLDELRQRMQEKGYLKDDEESEAE"
     gene            complement(2258..2899)
                     /gene="rpsD"
                     /locus_tag="AC16V7_17620"
     CDS             complement(2258..2899)
                     /gene="rpsD"
                     /locus_tag="AC16V7_17620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008339733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIR6258999.1"
                     /translation="MAKYRGPVCKLCRREGEKLFLKGERCFTPKCAFERRGYPPGQHG
                     KGAQFRRRRESDFNRQLRAKQKARRVYGVLERQFRRYYETSLSRRGLTGLNLLQILES
                     RFDNVVFRLGFADSRAQARLLVTHGHFAVNGRRTDIPSMLLSPGDEISVREGNSRTRP
                     YFKDLAAVAETKNIPAWLERDLKALSGKVLRLPERAEIDGNLHEQLIVEYYSR"
     gene            complement(2931..3332)
                     /gene="rpsK"
                     /locus_tag="AC16V7_17625"
     CDS             complement(2931..3332)
                     /gene="rpsK"
                     /locus_tag="AC16V7_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014015761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIR6259000.1"
                     /translation="MAQSVRTARRKGTKKIKRTLSSGQIHILATFNNTIITVTDPSGN
                     TVAWGSAGSAGFKGSRKSTPFAARLAAEQALKAAMALGLQEVQVFVKGPGPGRESAVR
                     AIQTLGLKVTSISDVTPVPHNGCRPPKKRRV"
     gene            complement(3356..3745)
                     /gene="rpsM"
                     /locus_tag="AC16V7_17630"
     CDS             complement(3356..3745)
                     /gene="rpsM"
                     /locus_tag="AC16V7_17630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HIR6259001.1"
                     /translation="MARIEGVDLPRNKRVEVGLTYIYGIGLPRAQQILKATGVNPDIR
                     IKDLTDADVTLLREFISQNYKLEGDLRREVQLNIKRLIEIGSYRGLRHRRNLPVRGQR
                     TRTNARTRKGPKKTVAGRGRRRGAKKK"
     gene            complement(3752..3865)
                     /gene="rpmJ"
                     /locus_tag="AC16V7_17635"
     CDS             complement(3752..3865)
                     /gene="rpmJ"
                     /locus_tag="AC16V7_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HIR6259002.1"
                     /translation="MKVSASIKKRCSKCKIVKRKGVLYVICENSKHKQRQG"
     gene            complement(4003..4773)
                     /gene="map"
                     /locus_tag="AC16V7_17640"
     CDS             complement(4003..4773)
                     /gene="map"
                     /locus_tag="AC16V7_17640"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIR6259003.1"
                     /translation="MISQRGIFIKSQQEITIMREAGRINALALAAVRQAIRPGVTTAE
                     LDQIAADVIRKHGAKPAFLGVPGAYPYPATTTISVNEEMVHGIPGKRKLQEGDIISVD
                     CGTIFEGYVGDSAFTMGVGEVSQVAQQLMEVTEKSLHIGIDQLRPGKRVGDVGAAIQE
                     YVEGQGFHLTREYTGHGVGRAMWEGPQVPNYGVSGRGTVFRPGMVIALEPMVLVGTTH
                     TRVLPDQWTVASADGSLTAHFEHSVAVTQNGPLVLTIP"
     gene            complement(4776..5432)
                     /locus_tag="AC16V7_17645"
     CDS             complement(4776..5432)
                     /locus_tag="AC16V7_17645"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIR6259004.1"
                     /translation="MPKYIVLLGPPGAGKGTQAQIISNELGLPHISSGDLFRENLKGQ
                     TELGKLAKGYIDRGELVPDDVTISMVRERLSRPDCSSGALLDGFPRTPAQAEALARML
                     DDFQSQVDVVPYISVPEPVLIERLTGRWTCRANGHVFHEKFNPPREAGRCDFDGSQLY
                     QREDDKEETVINRIHVYLKQTMPLIEYYRQQGLLLEVDGSQPIEKVSMDLLAALPAGK
                     "
     gene            complement(5445..>6054)
                     /locus_tag="AC16V7_17650"
     CDS             complement(5445..>6054)
                     /locus_tag="AC16V7_17650"
                     /inference="COORDINATES: protein motif:HMM:NF012564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR6259005.1"
                     /translation="YPGRRVGNRMSMPVKGTLPLMVNMAGMIPLIFAQSLLTFPAIIA
                     SFFVSSQIAWVSNTAATVQNLFGGNSNAYWIFYFLMVVGFTFFYTDVLFAQQNYGENL
                     KRVGAQIPGVSRGAPTQKYLTRVLRRITLPGALFLGLVAVLPVLVTLILSGFGVSLGG
                     QSGILLVSSAGLLIVVGVVRDTFFNIEAELKLHGYDDTQLIR"
BASE COUNT         1396 a         1754 c         1485 g         1419 t
ORIGIN      
        1 ttaacgcttc aagctcacgc cttccctgcc cgatctccac ctggaagctg accaatctcc
       61 gcaccatccg ataaagaaat gcatcagcag tgattataaa aatcagctcc tggtcatacc
      121 gtgtccattg tgcctgaaag acagtccgct gtgtgcttcc tgaagggcga ggcggggtac
      181 caaatgcggc aaaatcctgc cttccgacca agagactcgc agcctgttgg agcaagtcga
      241 gatccagggc aggccacacc cgccaggcgt atcgatccag caacgggtca cgattttccc
      301 agcaatagat ccggtagcga taagtgcggg caagcgcatc gtagcgagga tggaaatcca
      361 cctcaacttg cattacagac cgagcagcca catcgtctgg caattgcgca ttcagtgcag
      421 cacccaactc gtcaggggaa tgcttccaat ccagatcaaa agcaatcacc tgcccggagg
      481 catgcacacc agtgtctgtg cgcccagctg caagaatggc ccgaccactc cagcctaatt
      541 cccgcaatgt cgcctcaatc acaccttgca cagtatgtgc cttggcttgg cgttgaaagc
      601 catggtactg ggtgccatca taggcgagga taacttggta acgtgccatt ttcagttgtc
      661 ggtgatcaaa tgtcgcttag tcgaggacga taaatacggt gtagcaatca tcgatctctg
      721 acaactggcg attgactaga actcatctaa acatttctgg gttggggtag agacacccct
      781 cgctttttta gataaattct aatcttcgat aagctccaaa atcaccatat cagcagcgtc
      841 gcctaaacgc gggcctaggc tcagaatgcg cgtatagcca cccgggcgag attcaaaacg
      901 cggggcaatt tcatcgaaca gcttcttgac tgccacggga tcgcccaagc gggcagccgc
      961 caagcggcgg gcgtgcactg ctttctcctc accagcctgg ttgccgcgct tagcaagcgt
     1021 aatcaatcgc tctgcctggc ctcgcaccgc gcgagccttc gcgcgggttg tgcgaatgcg
     1081 ctcgtgctca aataattgct tgattaaaat tcgccgcaga gcggtgcgct catcctttcc
     1141 gcggctcaat ctatatccta cgattttgtg tcgcataccg tcactccgct tcgctctctt
     1201 cgtcgtcttt gaggtagccc ttctcctgca tcctctggcg taactcgtcc agacttttct
     1261 cgccgaaatt acggatggac atcaccgcct catcaccctt ttcaagcagc tcgatcacct
     1321 caccgatggt ggtgatgccc gtgcgcttga gtgagttgaa aacccgcacc gacaggtcca
     1381 ggttttcgat gggcgtctcg atgatctcgc tggtcagcct gcctgcctct tcttgggctt
     1441 ctgcgagcga ggcaagcgat tcttcgctga cacccgccag atggcgaaga tgatcaatga
     1501 gcgttttggc gccggtgctg agtgattcct ctggtccgat ggtcccatcc gtccagatct
     1561 ccatcaccag gctgtcatag tttgtgctct gacccacgcg cgccaagcca acatcccagt
     1621 tgacccgacg cacagggcta tagatggcat ctacgggtag ttcgccaatc ggcaagcgcc
     1681 cagtacgatc atctgcgggt gagtaacccc ttccccgttc aacagtcatc tcaatttcta
     1741 aattggcgtt gttatcatcc accgtgaaga ggtacagatc aggattaatg atctccactt
     1801 caggcggcgt gatgatatca gccgcggtga ccactcctgc acctcgtacc tctaggtgca
     1861 tgcgagcgga ttccacgtca ctcaatttca tgcgcaattg cttgatctgc aacattatcc
     1921 ggatcacatc ttcacggaca ccaggtacat cgctaaattc gtgttgcaca tccgcaaagc
     1981 gaattgaggt gaccgccgcg ccatcgagcg aggagagcag aacccggcgg agggcgttgc
     2041 ctaacgtgac accatagccg cgctccagcg ggctgataat gaacttgccg tagttgcgcg
     2101 cctccgcttt ccgctcaatt ttcggcgtta ccatattact taaacctatc acggttcacc
     2161 tcgaaattga ttgaagacag gcaggttaca agctaaagat ataccaggac aagctatggc
     2221 gtaatcaata actttcaacc ttccagcttg caatccatta tctagaatag tactcaacga
     2281 tgagttgctc gtgcaaattc ccatcgatct cagcccgctc cggcaggcgc aataccttgc
     2341 ccgatagagc cttaaggtct ctttccagcc aggcggggat atttttcgtc tcggccacgg
     2401 ctgctaagtc cttgaagtat gggcgggtac gagagttgcc ttcacgcaca gagatctcgt
     2461 cgccgggaga gagcaacatc gaagggatgt ctgtgcgccg tccattgacg gcgaagtgcc
     2521 cgtgtgtcac caagaggcgc gcctgagcac ggctatcggc gaagcccaga cggaatacca
     2581 cattgtcgaa gcgtgactcg aggatctgca acaagtttag acctgtcaac ccacgacgac
     2641 ttagagaggt ctcatagtaa cgacggaact gtcgctccaa cacgccatac acgcggcgag
     2701 ctttctgctt ggcgcgcaac tggcgattaa agtctgactc gcgcctacgg cggaactgag
     2761 cgcctttgcc atgctgaccg ggggggtatc cccgtcgctc aaaagcgcat ttcggtgtga
     2821 aacagcgctc ccctttcagg aacagctttt caccttctcg ccggcacaac ttacaaactg
     2881 gcccacgata ctttgccata cgtttcttac cttatccttc ctgaataata ttaaacacgc
     2941 cgcttctttg gtggtcggca gccattatga ggtactggcg tgacgtcaga aatcgatgtc
     3001 actttcagac ccagcgtctg gatggcgcgc accgccgatt cgcgacctgg cccaggacct
     3061 ttgacaaaga cctgtacctc ttgcaaaccc aatgccatgg ctgccttaag agcttgttca
     3121 gccgccaggc gggctgcgaa gggtgtgctt tttcgtgaac ctttaaaacc tgcagatcct
     3181 gcgctacccc aagccactgt attaccggac gggtcggtta ccgttatgat cgtattattg
     3241 aaggtagcca gaatatgaat ctgccccgat gatagggttc gtttgatttt ctttgtgcct
     3301 ttgcggcgag ctgtacgaac actttgagcc atagaacctc acaatttcca gatgattatt
     3361 tcttctttgc tccacgtcgc cgtccgcgcc cggcaactgt tttctttgga cctttgcggg
     3421 tgcgggcgtt ggtgcgcgtc ctttgcccac ggaccggcaa attgcgacga tggcgaagcc
     3481 cgcggtagct gccaatctca atcaagcgct tgatattgag ctggacttcg cgtcgcaggt
     3541 caccctctag cttatagttc tgagagatga attcgcgcaa cagggtgaca tcagcatcgg
     3601 tgagatcttt aatgcgaata tcagggttta caccggttgc cttcaagatc tgctgcgcgc
     3661 ggggcaggcc aataccataa atataggtca gacccacttc aacccgcttg ttacgaggta
     3721 agtcaacgcc ttctatacga gccatatctg attatccctg tcgctgttta tgtttcgaat
     3781 tctcacaaat gacgtataaa acgcctttgc gtttgacaat tttgcacttc gaacagcgct
     3841 ttttaataga tgcggatact ttcatttcat ttctccttct tattaaaact cagaaagagt
     3901 tccattctcc tgatagccga agttttcatt ataccgtaat cactttctct cgtccagttt
     3961 tctcccaaga atgcgctatt attcacagcg ggtttgctca actcaaggga ttgtcaacac
     4021 aagcggtcca ttctgggtaa ccgccacgct gtgctcgaaa tgagcagtca gcgaaccatc
     4081 ggcagacgcc actgtccatt ggtcgggtaa gacacgcgtg tgggtcgtac ccaccaatac
     4141 cattggctcc aaggcaatga ccatacccgg tcggaagacc gtgcccctgc cggaaacacc
     4201 gtaattgggc acctgcggac cttcccacat cgcccgtcct acaccatgac cggtatattc
     4261 ccgggttaag tgaaatccct ggccctctac atactcctgt atggcagctc ctacatcacc
     4321 aacgcgctta cccgggcgca actggtcaat accaatgtgc agagatttct cggtcacctc
     4381 catcagctgc tgggcaacct gagagacttc gcccacaccc atggtaaaag ccgagtcacc
     4441 tacgtatccc tcgaagatcg tgccgcaatc cactgagatg atatcgcctt cttgcagctt
     4501 tcgcttcccc ggtatgccgt gaaccatctc ttcgttaacg ctaatggtcg tcgtagccgg
     4561 gtatggatac gctccaggaa ctcccaggaa agctggtttg gcgccatgct ttcgaatgac
     4621 gtccgcagcg atctgatcca actcggcagt cgtcaccccg ggtcgtatcg cctggcgcac
     4681 agcagccaaa gccaaggcgt taatccgtcc agcctcgcgc atgatggtga tttcctgttg
     4741 gctcttgatg aagatgccgc gttgactgat catgtctatt tgcctgcagg gagagcagcc
     4801 aataaatcca tcgatacctt ctcgataggt tggcttccgt ccacttccaa tagtaagccc
     4861 tgttggcgat agtattcgat caggggcatc gtttgtttaa ggtacacatg gatacggttg
     4921 atcaccgttt cctctttatc atcctctcgc tggtagagct gcgaaccgtc gaaatcacaa
     4981 cggcctgctt cccgcggggg gttgaacttc tcgtgaaaga cgtgcccatt tgcccggcaa
     5041 gtccagcgtc cggtcaggcg ctcgatcagc acgggctccg gaacactgat ataaggcacc
     5101 acatctacct gactttgaaa atcgtccagc attctcgcca gggcttcggc ttgcgccggc
     5161 gtgcgtggaa atccatccag cagcgcgccc gatgaacaat ctggacgcga gagtcgctcg
     5221 cgcaccatgg agatggtcac atcatctggc accagctcac cccggtcgat atagcccttg
     5281 gctagcttgc ctaactcggt ctgccccttt aggttctcgc ggaacagatc tccagacgag
     5341 atatgcggca accccagttc attggagatg atttgcgcct gggttccctt acctgcaccc
     5401 ggagggccga gtaaaacaat gtactttggc atgtgtcact ccgcttaacg gatcagctgc
     5461 gtatcatcgt aaccgtgaag cttgagctct gcctcaatat taaagaacgt atcgcgcacg
     5521 acacccacca caatcagcaa tcctgctgat gagaccaaca ggatgcccga ttgaccgccc
     5581 agactgaccc cgaaaccaga cagaattaag gtgaccagca ccggcaaaac cgccaccaga
     5641 cccaggaata aagccccagg tagggtgatg cgccgcaaga cacgcgtcag atatttttgg
     5701 gtaggcgcac cccggctgac acccgggatt tgtgcaccga ctcgctttaa attctcgccg
     5761 taattttgtt gggcgaaaag cacatcagtg taaaagaaag tgaaacctac aaccatcaag
     5821 aagtaaaaga tccagtaggc attgctattg ccgccaaaca gattttggac cgtggccgca
     5881 gtatttgaaa cccacgcaat ttgtgaactg acaaaaaagc tggcgataat ggctgggaag
     5941 gtgagcaacg actgggcgaa gatcagcggg atcatgccag ccatgttgac catcaagggc
     6001 aaggttccct taaccggcat cgacatgcga ttgcctaccc ggcgtccagg atac
//
LOCUS       DBNHOH010000973         2097 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1473673, whole genome shotgun sequence.
ACCESSION   DBNHOH010000973 DBNHOH010000000
VERSION     DBNHOH010000973.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1473673"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(218..1756)
                     /locus_tag="AC16V7_17655"
     CDS             complement(218..1756)
                     /locus_tag="AC16V7_17655"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF012589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - obsolete D-gluconate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HIR6259006.1"
                     /translation="MTENARTDWFLGIVLGTGSCKSVIIDAQARLLGFGTGGYTRREN
                     TGKWEEQDPQELVSAMIQSVRAAIQNARVNPSACQAFSIGGAYHSLIAIDKADRPLTG
                     VITWVDDRATGQAQQVRHTHDPLKIYQQTGCPVHSMYPVYKLIWLREEQPQVFKQAVR
                     YISAKEYVTEQLTGQYLVDTGIAAGTALLNTRSLEWNAASLDLAGIRPEQLSPLVSPR
                     KIIKGRNAELFAQMSIPKETPLVLGSADAVNSSLGAGAVLPGQATCMIGTSGAFRIIA
                     PRPMLDEQARSWCYAIDEGHWLVGGAINNGGLALTWLRDAFNQMLHSQTKDARISFDD
                     LVTSALEIPPGADGLICLPFFAGERSPYWNMNARGVFFGLTLDHDARHMARALMEGVA
                     YRLRSLRDVLIDLGADVKEIRASGGFTQSDLWPQIIANVLNHDLFIPASGETSSLGAA
                     FWALIGMNVVNRFEDLYPLVQVTNTYHPDQKVVRLYDSLYKIYMDLYFQLGGAFEQIS
                     GIGD"
     gene            complement(1771..>2097)
                     /locus_tag="AC16V7_17660"
     CDS             complement(1771..>2097)
                     /locus_tag="AC16V7_17660"
                     /inference="COORDINATES: protein motif:HMM:NF014841.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR6259007.1"
                     /translation="SEETRHMIGAAQLQAMKPTAYLINTARGALVDEEALYTALINKQ
                     ISGAALDVYNDEPPKGSPLLQLENVVVTPHIGAHTRESIERVGVMAAENVLRTLQGGQ
                     PHYRVV"
BASE COUNT          494 a          570 c          527 g          506 t
ORIGIN      
        1 caggatcgca cgccattttg gcatccacca ctgtacagtt tcgttatggg tccaagccag
       61 cgctgccagt ctacctgaag tgccggtccc caaaaagtca agacggccga gatggatgaa
      121 ttgttcacct ttattggcaa caaaaaaaca ggttcaccat actaatgatc gtagaccacc
      181 aaatatgctc ttatctaaga tgggtagaga tttctgatta atctccgatg ccagaaatct
      241 gttcgaatgc accccccaat tgaaaataaa gatccatgta aatcttatat aaagaatcgt
      301 acaagcggac aactttttga tcaggatggt atgtattcgt aacctgcacc agtgggtaaa
      361 gatcctcgaa gcgattaacc acattcatac ctatcaacgc ccagaatgct gcgcccaagc
      421 tggatgtctc tccacttgca gggataaaca ggtcatggtt gagtacattg gcgatgatct
      481 gcggccacag atcggattgg gtgaagccac cagaagcacg gatttctttt acgtctgctc
      541 ccaaatcgat aagcacgtca cgcaggctgc gaaggcggta agcgacccct tccatcaacg
      601 cccgcgccat gtggcgtgca tcgtggtcta gggttagccc aaagaagacg ccacgagcgt
      661 tcatattcca atacgggctg cgctcaccag cgaagaaggg gagacagatc aaaccgtcag
      721 cgccaggcgg tatttctaaa gcggatgtga ccaggtcatc gaatgagatg cgagcgtctt
      781 ttgtttggct gtgtagcatc tggttgaagg catcccgtag ccaggtaaga gccagcccac
      841 cgttgttgat ggcacctcct accagccaat gaccctcatc gattgcatag caccagctgc
      901 gggcttgttc gtctagcata ggacgcgggg caatgatgcg gaatgcgccg ctggtaccaa
      961 tcatgcaggt cgcctgacct ggtaagactg caccagcgcc caaactggaa ttaaccgcgt
     1021 cggcagagcc gagcacaaga ggcgtttctt tcggaatgct catctgcgca aatagttctg
     1081 cattacgacc cttaataatt ttacgcgggc ttaccaatgg ggaaagctgt tcagggcgta
     1141 tccccgccag gtctaacgaa gcagcattcc attccaggct gcgcgtgttt aacagggctg
     1201 ttccagccgc gatgccagta tccaccagat actgcccggt taattgttcc gtgacatatt
     1261 ccttggcaga gatataccgg acggcctgct tgaaaacttg cggctgttct tcacgcagcc
     1321 aaatcagctt atatactgga tacatgctgt ggacgggaca gccagtctgt tgatagatct
     1381 tgagcgggtc gtgcgtatgc cgtacctgct gtgcctgccc tgtggcgcgg tcatccaccc
     1441 aggtgatgac tcctgtcaat gggcgatcag ctttgtcaat ggcgatcaag ctatgataag
     1501 caccaccgat gctgaaagcc tggcacgccg aagggtttac cctagcattt tggatggcag
     1561 cccttactga ttggatcatg gcggaaacaa gctcttgcgg gtcttgctcc tcccattttc
     1621 ctgtattttc acgtctggta tatccccccg taccaaagcc aagcagtcga gcctgtgcat
     1681 caatgatgac actcttgcaa ctgcctgtgc ccaggacgat gcctaagaac caatcggtgc
     1741 gtgcgttttc agtcatccct gtttatttat tcacacaact ctatagtgtg gctgaccacc
     1801 ttgtagagtc cgcagcacgt tttcagctgc catcacgccc acacgctcga ttgattcgcg
     1861 cgtatgcgcg ccgatatgcg gtgtaacgac cacattctcc aattgcagca agggactgcc
     1921 tttcggtggc tcgtcgttgt acacatccag ggctgccccc gaaatttgct tattgataag
     1981 tgcggtgtac agcgcctcct catcgaccaa cgcgccgcgt gcggtgttga tcagataagc
     2041 ggttggcttc atggcttgca gttgcgccgc gccaatcatg tgtcgcgtct cttcact
//
LOCUS       DBNHOH010000974         2422 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1151959, whole genome shotgun sequence.
ACCESSION   DBNHOH010000974 DBNHOH010000000
VERSION     DBNHOH010000974.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1151959"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..1069
                     /locus_tag="AC16V7_17665"
     CDS             98..1069
                     /locus_tag="AC16V7_17665"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR6259008.1"
                     /translation="MSKITMREAISKALWEEMERDSKVFILGEEVGVWGGTYAVTKGF
                     VDHFGPDRVRDTPIAEAAIVGAAIGAALTGLRPVAELMTINFAFSAMDHIVNEAAKLH
                     YMFGGQLVLPVVIRTVSGGGRQLGATHSQTPDAIFAHFPGLKVVAPGTPKDAKGLLKS
                     AIQSDDPVLFIEHATLYQVRGEVPDGDYTIPIGKSKIQRPGRDVTIVTYSKMLEISTK
                     AAEQLSQEGIEVEIVDLRSLRPLDMEPVLESFKKTNRAVIVEEGWRSYGVGSEVAARI
                     YEQAFDYVDAPIQRVAQEEVPLPYNRNLEQLALPQVEDVVRAVKEVL"
     gene            1069..2316
                     /locus_tag="AC16V7_17670"
     CDS             1069..2316
                     /locus_tag="AC16V7_17670"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010507946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HIR6259009.1"
                     /translation="MAETVTMPKLGFDMAEGTLIRWVIAEGEAVNKGAILAEIETDKA
                     TVEVESVYDGILARHLVAEGDIVPVNTPIAIVAAPGEKVEKQPQLVTDQAAPTPKSAA
                     EAEPISAPSQAPSEPTVVEGQLPEGMKASPLARRMAEERGIPLRSISGSGPGGRITKK
                     DVEAYKAVQAAPTQPQVAFQAGLPSIPSTTIPLKRLRAIIGRRMTEAKQSVPHFYVTH
                     EYDMAALLALRQQVNLTLPDEEKTSVNDFVLKASALTLREFPNLNASLDDNNNQIIQH
                     GAVNIGVAVAVEGGLLTVVCRDADRKPIRQISAEVRQMVQRAREGKVRPDDIEGSTFS
                     VSNMGMYDVEHFIAIINPPEAAILAVGSAREVPVVKDGEVVPGLRMKATLSVDHRVSD
                     GAEGARFMQTLARYIEEPIRLLL"
BASE COUNT          592 a          586 c          687 g          557 t
ORIGIN      
        1 ggtccagcag gctgttcagt tcgctgaggc aagccctgaa ccggctcccg aagcgttgtt
       61 tgagcatgtt tatgtagaag gataggaata ggggataatg tcaaaaatca ccatgcgaga
      121 agccatctcc aaagcacttt gggaggagat ggagcgagat tcaaaagtgt ttatcttggg
      181 tgaagaagtg ggggtttggg gtggcactta cgctgtaacc aagggtttcg ttgaccactt
      241 tgggcctgac agggtgcgcg ataccccaat cgctgaggcg gcaattgtcg gcgcagccat
      301 cggggctgct ctgactggat tacgaccggt ggctgagctc atgacgatta attttgcttt
      361 ttccgctatg gatcatatcg ttaatgaggc ggctaagctg cattacatgt tcggcgggca
      421 attggtgttg ccggtggtta tccgaacggt tagcggcggt gggcgacaat tgggagctac
      481 ccattctcaa actccggacg caatttttgc ccactttccc ggattgaagg tggttgcccc
      541 tggaacaccc aaggacgcca aaggactgct aaagtctgcc atccaaagtg atgaccctgt
      601 cttattcatt gagcatgcca cgctatacca ggttcggggc gaggtgccgg atggcgatta
      661 cacgatcccc attgggaagt caaaaattca gcgacctggc cgagatgtga ccatcgttac
      721 ctacagcaag atgcttgaaa tctcgaccaa ggcagctgag cagctttccc aagagggcat
      781 cgaagttgag atcgtcgacc tgcgctctct gcgaccattg gatatggagc cagtgctcga
      841 gtcattcaag aagaccaatc gtgccgtgat tgtggaagaa ggctggcgat cttatggggt
      901 gggttctgaa gtggctgccc gtatttatga gcaagctttc gattatgtcg atgcccctat
      961 ccaacgggtt gcccaagaag aagtgccctt gccatataac cgtaatttgg aacaactggc
     1021 tttgcctcag gtggaagatg tagttcgtgc ggtgaaggag gtactctgat ggcagaaacc
     1081 gtcacgatgc cgaaactagg ttttgatatg gctgaaggca ccctgatacg atgggtgatt
     1141 gctgaaggtg aggcggtgaa taaaggggcg atcctggcgg agattgagac cgataaggcg
     1201 actgtggagg ttgagtcagt gtatgatggc atcctggcca gacaccttgt cgccgaaggc
     1261 gatattgtac cggtaaatac acccattgca attgtcgctg cccccggaga gaaagttgaa
     1321 aaacagcctc aattggtaac cgaccaagct gctccgaccc caaaatcggc agcggaagcc
     1381 gaacctatca gcgctccttc ccaggcgccc agcgaaccaa ccgtggtcga aggacagctc
     1441 cccgaaggga tgaaggcttc acccctggca cgccgcatgg cggaagagcg gggtatccct
     1501 ttgagatcca tctcaggtag cggtcctggc gggaggatca caaagaaaga tgtggaggct
     1561 tacaaagctg tgcaagccgc accaacccaa cctcaagtag cctttcaagc cggcttaccc
     1621 tcgattcctt caaccaccat tccgttgaag cgcttgcgag ccatcattgg gcggcgtatg
     1681 acagaagcca aacaaagcgt tccgcatttt tacgtgacac atgaatacga tatggcagct
     1741 ttactggcat tacgccagca ggtcaacctg actctgcctg acgaagagaa aacctctgta
     1801 aacgactttg ttctaaaagc ttctgcgctt acccttaggg aatttcccaa tctgaatgct
     1861 tcgctggacg ataacaataa ccagatcatc caacatgggg cagtaaatat cggtgtggcg
     1921 gttgccgtcg aaggcggttt gctgaccgtg gtttgccggg atgctgatcg caaaccaatc
     1981 cggcagatat ctgccgaagt gcgtcagatg gtgcagcgtg cccgagaagg caaggtgcgc
     2041 ccagatgata tcgaagggtc tacattctcg gttagcaata tgggaatgta tgacgtggag
     2101 cactttatcg ccatcatcaa tccccctgaa gcagccatct tggccgtagg atcggcgcgt
     2161 gaggtgccgg tggtgaaaga tggggaagtt gtgcccggat tgcgcatgaa agcaactctc
     2221 tcggtggatc atcgcgtcag cgatggagct gaaggtgctc gcttcatgca gaccttggca
     2281 aggtatatcg aagagcccat ccggttatta ctttagaaaa gagcaactcc ctacaaaaac
     2341 ttgtagggag ttgtttcttg tatagcttca ttcattgtag cacttacttg aatgcgtaca
     2401 cgactgattc ctcatcgcgg ta
//
LOCUS       DBNHOH010000975         2787 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1280409, whole genome shotgun sequence.
ACCESSION   DBNHOH010000975 DBNHOH010000000
VERSION     DBNHOH010000975.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1280409"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2383
                     /locus_tag="AC16V7_17675"
     CDS             <1..2383
                     /locus_tag="AC16V7_17675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cellobiose phosphorylase"
                     /protein_id="HIR6259010.1"
                     /translation="GYPAKLAPAESVTLYSLFGYASNIEVLKEVAPRLGADGYFSGKR
                     SFANKLVSEITAPIETRTSSPVFDAYCKQTFLDNVLRGGWPVHLGTVEKPITYHIYSR
                     KHGDPERDYNAFFLAAEYYSQGNGNYRDVNQNRRCEVLFDPRVGDANVHSFMSLIQLD
                     GYNPLVVKGSWFWIPPERRNSILKLTVQPERMEQFLDKPFTPGSLLKWIDENEVGLHI
                     STSIFLQEALQQAEQYFDAEFGHGYWIDHWTYNLDLINSYLAVFPDRGSELLFGKGDL
                     PFFESPVFVQPRSKKYVLAGEHPRQYGAVVQDEEKAALITARRDYPNLMRTQKGHGSV
                     YRTSLYAKLVLLALIKFATLDPLGLGIEMEAGRPGWCDALNGLPGLFGSSLSETFELA
                     RLLIFLHEHLPKSMSGSVDLPVEVSDLLQSVVNCLRAYHNSDQKERDFIYWDSVSNAR
                     ELYREKIRFGIDGEVVPITFQVLAGYLSLFLDKVRSGIERALELNGGIPPTYLTYDVE
                     KYDILYGIDNKPLKDAKGRSYIRALRFQPRFLPVFLEGPVRMLKTLPDSHAARQLHQQ
                     IKGSTLYDRSLQMYRTNAPLDNEPVEIGRLRAFTPGWLENESIFLHMEYKYLLELLRA
                     GLYEEFFEDFRNALVAFQDPARYGRSPLENSSFIVSSAHPDKNLHGAGFVARLTGATA
                     EFLSMWNIMMAGQNPFFIDKDELCLRLQPALPHWLFDEAGTLSFTFLGNCRVTYYNPR
                     REDTFAISNQIQKLHLYLDDKQVEVEGDIIGSPYASFVREGRIKRIDVFFAEV"
     gene            2417..>2787
                     /locus_tag="AC16V7_17680"
     CDS             2417..>2787
                     /locus_tag="AC16V7_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH36-type glycosyl hydrolase domain-containing
                     protein"
                     /protein_id="HIR6259011.1"
                     /translation="MIQTWKFIDSQGTFELQNPHHSSYLYFPLANEAGMMSAVTPLLH
                     GDIKAGQHSFLTPPVSVEDLHNIRSARNFWVHIPGIGAWSATGNSAAQIAKNFSGQED
                     DEIALKAGFLWHQVFRQNKNLG"
BASE COUNT          708 a          659 c          694 g          726 t
ORIGIN      
        1 tggctatcct gccaagctcg ctcctgctga gtcagttact ctatacagcc tctttggata
       61 tgccagcaac atagaagtgc tcaaagaggt tgcaccgcgt ctgggcgcgg atgggtattt
      121 ctccggaaaa aggagctttg ccaacaaatt agtgagcgaa ataactgcac caattgaaac
      181 caggaccagt tcgcccgtct ttgacgcgta ttgcaagcaa acatttttag ataacgtcct
      241 gcgcggtggt tggccggttc atctggggac cgttgagaaa ccaatcacat atcatattta
      301 ttcgcgcaaa catggggacc cggagcggga ttacaatgcc tttttcttag cagcagaata
      361 ctactcgcaa ggtaatggaa actatcgcga cgttaatcaa aatcgccgct gcgaggtttt
      421 gtttgacccc cgcgtagggg atgccaatgt acactccttc atgagcctga tccaacttga
      481 tggttacaat ccgctggtgg tgaaaggtag ttggttctgg atcccacctg aaagacgaaa
      541 ttcgatcctc aagctgactg tccaacctga gcgcatggag caatttctag ataagccctt
      601 tacgcccgga agtttactca agtggataga tgaaaatgag gtgggtctgc atatatccac
      661 gagtattttc ctgcaagagg cactgcaaca agctgaacag tattttgatg ctgaattcgg
      721 acacggatat tggatcgatc attggaccta taacctggat ttgatcaaca gctacctggc
      781 tgtttttcca gaccggggaa gcgagttgct ctttggcaaa ggcgacttgc cattttttga
      841 aagcccggtc tttgtgcagc cacgcagcaa gaaatatgtg ctggcaggtg aacatccccg
      901 ccaatatggc gcggtagtac aagacgaaga aaaagctgcc ctgatcaccg cccgccgaga
      961 ttatcccaat ttgatgcgta cacagaaggg tcatggatcc gtgtatcgga ccagtttgta
     1021 tgctaagctg gttttgttgg cgttgattaa attcgccacg ctggatccac ttgggttggg
     1081 catcgaaatg gaggcggggc gaccaggatg gtgtgacgcg ctgaatggcc ttccagggtt
     1141 gtttggctca tccttgtcag aaacttttga gctggcgcga ttgttgatct tcttgcatga
     1201 acatcttccc aaaagcatgt caggttctgt agacctacct gtagaagttt cagatctcct
     1261 ccaaagtgtc gtcaattgct tgcgggctta ccacaactct gatcagaaag agcgtgattt
     1321 tatttactgg gatagcgttt ccaatgcccg cgagctctac cgggagaaaa tacgatttgg
     1381 catcgatggc gaggttgtgc caataacttt ccaagtattg gcgggatact tatcactatt
     1441 cctggataaa gtgcgaagcg gtatcgagcg tgccttagag ttaaatgggg gtattccacc
     1501 tacctacctc acgtatgatg tcgagaagta tgacatcctg tatggaatag ataacaagcc
     1561 tctaaaagat gcaaaagggc gttcgtacat ccgcgccctc cggtttcagc caaggttttt
     1621 acctgttttc ctagaaggac ctgtgcggat gctcaaaaca ctgccagact ctcatgcagc
     1681 tcgccagtta catcaacaaa ttaagggcag cacgttgtat gaccgttccc tacaaatgta
     1741 cagaacaaac gctcccctgg ataacgaacc tgtggagatc gggcgcttac gagcgtttac
     1801 acccggttgg ttggaaaacg aatccatctt tttacacatg gagtacaaat acctgctcga
     1861 gctcttacga gccgggttat atgaagaatt cttcgaggat tttcgcaatg cattggttgc
     1921 ctttcaagat ccagccagat acggacgtag ccccttggag aattcatcct ttattgtcag
     1981 cagcgcccac cccgacaaaa acctgcacgg tgccggcttt gtcgctcgcc tgaccggagc
     2041 gacagcagag tttctaagta tgtggaatat catgatggca ggtcagaatc catttttcat
     2101 tgataaagat gagctctgcc tgcgtttgca acctgctctg cctcattggc tgttcgatga
     2161 ggctggcacg ttaagcttca cattcctcgg aaattgccga gttacctatt acaaccctcg
     2221 acgggaggat acttttgcga tcagtaatca gatccaaaaa ttgcatctct acttggatga
     2281 caagcaggtt gaggtagagg gagatatcat cgggtcacct tatgcatcct ttgtccgcga
     2341 ggggcgtatc aagcgcattg atgttttctt tgcggaggtt taactgaaat tcctaccagg
     2401 tatggaggat gccagaatga ttcaaacgtg gaagttcatc gactctcagg gcacttttga
     2461 gctacagaac ccacatcact caagttatct gtatttccct ctcgccaatg aggccgggat
     2521 gatgtcggcg gtaacgccat tgctacatgg ggatatcaag gctggacaac actccttcct
     2581 gacgccgcca gtctcagtgg aagacctgca caacattcgc tctgcgcgta atttttgggt
     2641 tcacattcca ggtatagggg catggtcagc aacaggcaat agtgctgcac agattgctaa
     2701 aaacttttcg gggcaggaag atgatgaaat cgccctaaaa gctggttttc tatggcatca
     2761 agtgtttcgc cagaataaaa accttgg
//
LOCUS       DBNHOH010000976         1150 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1665116, whole genome shotgun sequence.
ACCESSION   DBNHOH010000976 DBNHOH010000000
VERSION     DBNHOH010000976.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1150)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1665116"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="AC16V7_17685"
     CDS             complement(<1..903)
                     /locus_tag="AC16V7_17685"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR6259012.1"
                     /translation="MDIAFKGKGVHNAIQRIGVISQRYGLTSGKMEAILARFASILRQ
                     FDCSATFPIPASALVRSNGMIEKYQQMGIEFAVHGYHHVDHTSLSLPEQLSYFERARR
                     VFESRGLVCNGFRSPYLRWNEATLTALNQAGFLYEGSQGLAWDVVNGVDTASYRHVLN
                     FYGAISATNYPALPRWENGLVRIPYCLPDDEALVDRFHLEAGDMMSKLWLAILDKTYA
                     LGELFTLGLHPERIELCEAPLIATLQKARALSPAVWIARLDEIARWWVERSHTVVTVT
                     PGEEGELSVSVRGPIGVTILTRGVS"
     gene            complement(967..>1150)
                     /locus_tag="AC16V7_17690"
     CDS             complement(967..>1150)
                     /locus_tag="AC16V7_17690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6259013.1"
                     /translation="LTTDLLSGRPGLNRVDLLMNTVEVATTLGYICLDELAFADRRIG
                     QGLEFTQEVYLPIITR"
BASE COUNT          267 a          317 c          305 g          261 t
ORIGIN      
        1 gctgacccct cgggtcagaa tggttacgcc aatcggtcct ctaacgctga cggaaagctc
       61 gccttcctca cccggggtaa ccgtcacaac cgtatggctg cgctctaccc accagcgggc
      121 gatctcatcc agcctggcga tccagactgc aggtgacagc gcgcgggctt tttgcaatgt
      181 tgcgatcaat ggggcttcgc aaagctcgat gcgctccggg tgcagaccca gtgtaaacaa
      241 ctctcctagc gcgtacgtct tatccaagat cgccagccag agcttgctca tcatgtcacc
      301 tgcttcgagg tgaaagcgat cgacaagcgc ctcgtcgtcc ggcaggcagt aagggatgcg
      361 caccaggccg ttctcccaac ggggcaaggc cgggtagtta gtagccgaaa tggcgccgta
      421 aaaattcagg acgtgtcggt aggatgcggt atccacgccg tttactacat cccatgctaa
      481 gccctggctg ccttcataga ggaagccagc ttggttaagc gccgtgaggg tggcctcatt
      541 ccagcgcagg tacgggctgc gaaatccatt gcacaccaag cctctgcttt cgaagacacg
      601 gcgcgctctc tcgaaatatg ataactgctc aggtagtgac agggaggtgt ggtcaacgtg
      661 gtggtagcca tgcacggcaa actcaatccc catctgctgg tatttttcga tcatcccgtt
      721 gctgcgcacc agcgccgagg cagggatagg aaaagtggct gagcaatcga actggcgtaa
      781 tatagaagca aaacgagcca agatggcttc catcttcccc gatgtcaggc catatcgctg
      841 gctgattacg ccgattcgtt ggatagcatt atgaacgccc ttacctttaa aggcaatatc
      901 cattggtttc atcgtatcta attaaacctg taaggaatac tccggatact ttttttggat
      961 cgctggttat cttgttataa ttggcaggta gacctcttgc gtaaattcca acccttgacc
     1021 tatccgccgg tcggcaaaag caagttcatc gaggcagata taccctagtg tagtagcaac
     1081 ttccacagtg ttcatgagta ggtccacccg gttcaagccc ggacgcccgg aaagtaaatc
     1141 tgtggtcagg
//
LOCUS       DBNHOH010000977         1710 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_61869, whole genome shotgun sequence.
ACCESSION   DBNHOH010000977 DBNHOH010000000
VERSION     DBNHOH010000977.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_61869"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..547
                     /locus_tag="AC16V7_17695"
     CDS             149..547
                     /locus_tag="AC16V7_17695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR6259014.1"
                     /translation="METLTLELQTMYGDHHVLEVRRLMLEMPGVEDVYASSGFRVVEI
                     AYDPAKVAPEALRSKLSEAGYLGDLPVPVEAGAAVYVEKGDRSFFRHTTVYENTQQVV
                     SFAQNVGYSGRPLWPCPGLGPIQAKNLEEE"
     gene            550..>1710
                     /locus_tag="AC16V7_17700"
     CDS             550..>1710
                     /locus_tag="AC16V7_17700"
                     /inference="COORDINATES: protein motif:HMM:NF015050.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HIR6259015.1"
                     /translation="MARARRSYEEQSSDPAAQEMLIHADELGLSTAFTRADDMVPCNI
                     GAAGMCCKLCGMGPCRLTKDGQTGVCGATIDTIQARNLIRAIAAGSAAHSDHGRDMAF
                     TLKAVANGEAEGYVLRDIAKLRTVAAEYGIPIEKRSPQEIANDLADLYIAQFGQQKGS
                     ISPVIRAPKKRQKLWEEQGVIPRGIDREVVEALHRTHIGDDQDPEHILQHAIRTGLAD
                     GWGGSLIATDVSDILFGTPAPILGQANLGVLKDDMVNVVVHGHEPTLSQMIVAATQDP
                     EIIEYAKAAGANGVNVSGICCTANEILMRQGIPAAGNFLHQELAILTGAVEAMVVDVQ
                     CIMQALVELASNFHTMVITTSPKVKIKGSVHIEFNEHKALTIAKQILKTAIDN"
BASE COUNT          450 a          367 c          477 g          416 t
ORIGIN      
        1 cattggagca gtttttatgg caggtaaaca atcgagccag cctgaatttc acctgtagta
       61 attgctatag gtgtgaatcc aggcacattt ttgtatggaa gtaattattt catcaacgaa
      121 atcaaaaaag agataaggaa ggagaatcat ggaaactttg actctcgaac tgcaaaccat
      181 gtatggtgac caccacgtgc tcgaagtaag gcgcctgatg cttgaaatgc caggtgttga
      241 agatgtttac gcaagcagtg gatttcgagt agtcgaaatt gcatatgacc cagccaaggt
      301 agcaccagaa gctttgcgct ccaagttaag tgaagcaggg tatcttggag atttaccggt
      361 tcccgtggaa gctggggcag ccgtgtatgt ggaaaaaggg gatcgttcct tcttccgaca
      421 tacaacggtt tatgagaaca cccaacaggt ggtaagtttt gcgcagaatg ttggttattc
      481 tggtcggcct ctttggcctt gcccgggcct gggcccaatt caagcgaaga atttagagga
      541 ggagtaaaaa tggcgagagc taggcggtca tacgaagaac aaagttcaga tcctgctgcg
      601 caagagatgc tcattcatgc ggatgaactc ggtctgagca ctgcgttcac ccgcgctgat
      661 gacatggtac cttgcaacat cggtgcggcg ggtatgtgtt gtaaattatg tggtatgggt
      721 ccatgccggt taacgaaaga tggacagaca ggtgtttgtg gggctaccat tgacactatt
      781 caagcgcgca atcttatccg tgctattgct gcaggatctg cagcgcattc tgatcacggg
      841 cgcgatatgg catttacact caaagcggtg gcgaacggtg aagcggaagg ttatgtgctg
      901 cgtgatatag ccaaactgcg taccgtagct gcagaatacg gcatcccgat tgagaagcgc
      961 tcaccgcagg agatagccaa cgacttagca gacctctata ttgcccaatt cggccagcaa
     1021 aagggaagta tttctcctgt catccgcgca ccaaagaagc gccagaagct atgggaagag
     1081 cagggggtta taccgcgcgg tattgatcgc gaggttgtgg aggctttaca ccgtacccac
     1141 attggcgatg atcaggatcc agaacacatc ctacagcatg ctattcgcac cggccttgcg
     1201 gatggctggg gcggttcgtt gattgcaaca gatgtatcag atattttatt cggtacgcct
     1261 gccccgatct taggccaggc gaacttgggt gtattgaaag atgatatggt caatgttgtg
     1321 gtgcatggtc atgagccaac cctgagccag atgattgtgg cagcgaccca ggaccctgag
     1381 atcattgagt acgcaaaagc tgctggggcg aatggcgtca atgtctcggg tatttgctgc
     1441 acagccaatg aaatcttgat gcgccaaggt atcccggcgg cagggaactt cttgcatcaa
     1501 gaattggcga tattgactgg tgcggtagaa gctatggttg tggatgtgca gtgcattatg
     1561 caggcattgg tggaattggc cagcaatttc catacaatgg ttatcactac ttctccgaag
     1621 gtgaaaatta aaggctctgt tcacatcgag ttcaacgagc ataaagcttt gacgatcgca
     1681 aagcagatat tgaagaccgc catcgataat
//
LOCUS       DBNHOH010000978         5189 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_511334, whole genome shotgun sequence.
ACCESSION   DBNHOH010000978 DBNHOH010000000
VERSION     DBNHOH010000978.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5189)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_511334"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..1056
                     /locus_tag="AC16V7_17705"
     CDS             46..1056
                     /locus_tag="AC16V7_17705"
                     /inference="COORDINATES: protein motif:HMM:NF012742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR6259016.1"
                     /translation="MPTIREVAKRAGVSPITVSRVVNDSGYVSEKTRARVEAVIEELG
                     YVPNMLGPSLRFKQTKTLAVVITDITNPFWTTVTRGVEDVAQANGYSTILCNTDESET
                     KQEQYMQMLLRRQIDGILLVPASSKAEPIRMIQNQGIPVVLLDRQVNGIDVDIVRADS
                     EEGAYRLTQHLISLGHQNIAMLAGPKDVSTSVDRVNGYRRALEDAALKTNIDQIYWGN
                     FTQEAGYEMARIVADLMPRPTALFAANNFIAIGAILALKEKGLRVPEDMALVTIDDIP
                     PPYTLEPFLTVATQPAREMGQQAAQLLFARIKREGNLPCRQVVLPTEMFIRTSSGGCI
                     NA"
     gene            1247..2767
                     /locus_tag="AC16V7_17710"
     CDS             1247..2767
                     /locus_tag="AC16V7_17710"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR6259017.1"
                     /translation="MSKVLILMEGIEKRFPGVHALNQCRFELRSGEVHALVGENGAGK
                     STLMKILGGIYHKDAGSIYLEGQEVEIQNPRMAQELGISIIHQELNLMPHLTVAQNIF
                     IGREPRSNIPFIVDDRITNQHAERLFKMLNLQLDPVAKVADLTVAKQQMVEIAKALSF
                     NAKILVMDEPTAALTETEIDELFRIIRQLREKGVGIVHISHRLEELKQISDRVTVMRD
                     GKYIDTKRIQDVTIDQIISMMVGRTIYETAPELPENPDQDIVLEVRNINRGKVLKDVS
                     FNLKKGEILGFAGLIGAGRTELARAIFGADAYDSGEVIIKGQRVNIKSPGDAVTHGIG
                     YLSEDRKRYGLTLGMDVKENIAMAAMKNFLNWFGWVDFKKTVVRGQEMVKALNIKTPN
                     LEQQVKFLSGGNQQKVVIGKWLTADTEILIFDEPTRGIDVGAKSEIYKLLNDLAHQGK
                     AIIMISSELPEILRMSHRVVVMCEGRVTGILNAGEATQESIMKYATMRNAVVKASA"
     gene            2805..3791
                     /locus_tag="AC16V7_17715"
     CDS             2805..3791
                     /locus_tag="AC16V7_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015846877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR6259018.1"
                     /translation="MNSEPKTAMTLFRSDAMQRIMAFGALIVLAGGFSLASPFFRTFD
                     NFVGILLATAVNGVLGLGATFVIITAGIDLSVGTVLTFSAVMTGVLITNLHLPIPLGI
                     LCGILAGGLAGLVNGLMISRMKIPPFIATLGMLYATKGLSLVISELKPIYFNDTPAFR
                     EMAMGSALGNIIPGFEVPNAVFIMFGAAIIANLILSRTILGRYTFALGSNEEATRLSG
                     VNVAFWKTAVYTLCGLFSGLGGVLMASRLNSAQPALGAGYELDAIAAVVIGGTSLSGG
                     EGSILGTIIGAFIISTLTNGLRILSVPQEWQMVVTGGIVILAVFLDILRRRR"
     gene            3922..4902
                     /locus_tag="AC16V7_17720"
     CDS             3922..4902
                     /locus_tag="AC16V7_17720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR6259019.1"
                     /translation="MNTKKVLFALLSALVLISVIAGCAPAAQPAAPAEPAAKPYIPVI
                     SKGFQHQFWQAVKLGAEQAATDLNVDITFEGPESEAMVDKQVEMFQTALDKKPAAICL
                     AAVDSKAFQNLLEKAKDAGIPVVGFDSGVDSDIPVTTAATDNIAAAALAADKMVELIG
                     GEGEVAVIAHDQTSRTGIDRVAGFVDQVKNKYPNVTVVDVQYGGGDQLKSTDLAKAII
                     QAHPDLKGFFGANEGSIIGVLNAVAELGMEGQITVIGYDSGQQQMDAIRAGTEAGAIT
                     QNPVGIGYKCVEAALKAYNGEELPKTIDTGFYWYDKSNIDEPQIQAVLYK"
     gene            4999..>5189
                     /locus_tag="AC16V7_17725"
     CDS             4999..>5189
                     /locus_tag="AC16V7_17725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU domain-containing protein"
                     /protein_id="HIR6259020.1"
                     /translation="MIKTGILNPQILSLISRVRHTNTLVIADRGFPYWPMIETVDISL
                     VDGIPTVLQVFKALKSNFI"
BASE COUNT         1313 a         1251 c         1339 g         1286 t
ORIGIN      
        1 atcatggtac acatatcata ataggcttgt aactgattag ccaaaatgcc cacaatccgc
       61 gaggttgcca aaagagctgg tgtgtcaccg attacggttt cgagagtggt taatgattcg
      121 ggctatgtaa gtgaaaaaac cagagcgcgc gttgaagccg taattgaaga gctgggatat
      181 gtccctaata tgttggggcc gagtttgcgc tttaaacaga cgaagacgct ggcggtggta
      241 attacagaca tcaccaaccc cttctggact acggtgacgc gtggcgttga agatgtggct
      301 caagctaacg gttattccac gatcctctgt aatacagatg aatccgaaac taagcaagag
      361 caatatatgc agatgctctt gagaaggcaa atcgatggca tattattggt tccggcttct
      421 agcaaagctg aaccaattag aatgatccaa aatcagggta tcccggtggt attattggat
      481 cgccaggtaa atgggatcga tgtagatatt gtacgggccg attctgaaga aggtgcctac
      541 cggctcacac aacatctcat ttctttggga caccagaata ttgcaatgtt agccggtccc
      601 aaagatgttt caacatctgt agaccgtgtg aacggttatc gacgggcgct agaggatgct
      661 gccctaaaga caaacattga tcaaatttat tggggcaatt tcactcaaga agctggttat
      721 gaaatggccc gaattgttgc agatttaatg ccgcgtccta cagctctgtt tgcagccaac
      781 aactttattg ctatcggtgc tatactggct ttgaaggaga aagggttacg agtgcctgaa
      841 gatatggcat tagttaccat agatgatatt cctccaccct atactcttga gccgtttctc
      901 actgtagcaa cccagccagc gcgtgagatg ggacagcagg cagcccaact cctatttgcg
      961 agaatcaaaa gagaaggcaa tctcccttgc cgacaggtcg tcttgccaac cgaaatgttc
     1021 attagaactt caagcggagg ttgtattaat gcttgaagtt ttttgggtcg ttgggagttg
     1081 ccgtggaaag tcccggattt aggagtggtc cgccacgtcg attcttaaag aaccggtttt
     1141 ttgtaatacg atgcgcctgc caaatcttgc ggtgtagaaa ttctacaaaa cgagcatatt
     1201 ttcgcaacgc gatcgagtga caaattcgtg acaaaaaggt tggtttatga gtaaagtgtt
     1261 aattctcatg gaggggatcg agaagcgatt tcctggcgtc catgcgctca accaatgtcg
     1321 cttcgaattg cgatctggtg aggtgcatgc actggtaggt gaaaacggtg cgggcaaatc
     1381 tacacttatg aaaatcctcg gtggaatcta tcataaggat gctggaagca tctacctcga
     1441 aggtcaggag gttgaaattc aaaacccccg tatggctcag gaactaggga tcagcatcat
     1501 tcaccaggaa ctgaacctga tgccgcatct gacggtggcg cagaatatat tcatcggtcg
     1561 cgaaccgcgt tccaatatcc cgtttatagt ggatgataga atcaccaacc aacacgccga
     1621 acgtcttttt aaaatgctta acctgcaatt ggacccagtc gcaaaagttg ctgacttgac
     1681 agttgccaag cagcaaatgg tggaaattgc aaaggcgctt tcattcaatg ccaaaatctt
     1741 ggtcatggat gaaccaacgg cagccctgac cgagacggaa attgatgaac tcttcagaat
     1801 tatcagacaa ttgcgcgaaa aaggtgttgg gattgtgcac atttcgcacc ggctggaaga
     1861 actcaagcaa atctctgacc gtgtgacggt tatgcgcgat gggaaatata tcgatacgaa
     1921 acgtatccaa gatgtgacca ttgaccagat catcagcatg atggtgggac gcaccatcta
     1981 cgagacggct cccgaactgc ctgaaaatcc cgatcaggat atcgttcttg aagtccgcaa
     2041 cattaatcgt ggaaaagtgc tcaaagatgt cagttttaac ctgaagaagg gtgagatttt
     2101 gggctttgcc gggttgattg gcgccgggcg caccgaattg gcgcgcgcta ttttcggagc
     2161 ggatgcgtac gactctggcg aggtcatcat caaaggccag cgggttaaca tcaaaagccc
     2221 cggcgatgcc gttacgcatg gcatcggcta tctctccgaa gaccgcaaac gttatgggtt
     2281 gacccttggc atggatgtca aagaaaatat tgccatggcg gcgatgaaga atttcctaaa
     2341 ttggtttggc tgggtggatt tcaaaaagac ggttgtccgc ggccaggaaa tggtcaaggc
     2401 actcaacatc aaaacaccca accttgaaca gcaggtgaaa ttcctctccg gcggtaacca
     2461 gcaaaaggtg gtcattggca agtggttgac cgccgatacc gagatattga tcttcgatga
     2521 gcctactcgt ggtatcgacg taggcgccaa aagtgaaatt tataaactgc tcaacgacct
     2581 ggcgcaccag ggcaaggcca tcatcatgat ctcatctgaa ctgccggaga tcctgcgcat
     2641 gagccatcgc gtggtggtga tgtgcgaagg ccgggtgacg ggtatcctga atgctggtga
     2701 ggccacccag gaaagtatca tgaaatatgc caccatgcgt aatgcggttg tgaaggcatc
     2761 tgcctgataa tgacgccatt cattgtgaca cgggaagaaa aactatgaat agtgaaccca
     2821 aaactgccat gaccctgttc cgttccgatg ccatgcaacg tatcatggct ttcggtgccc
     2881 tgatcgtgct agccggaggt ttttcgctgg catcgccatt tttccgcacc tttgataact
     2941 ttgttggcat tttgctcgcg actgcggtca acggtgtgct ggggttgggc gctacctttg
     3001 tcattatcac tgccggtatt gacctttctg tgggcacggt gctcacattt tcggcggtga
     3061 tgaccggtgt gctcataacg aacttgcatc tgcccatccc acttggcatt ctatgtggca
     3121 tcctggctgg aggcctggca gggttggtca acggcctcat gatctcgcgg atgaaaatcc
     3181 cgccttttat cgccacccta ggcatgttgt atgccaccaa gggcctttcg ttggtgatct
     3241 cggagttgaa accgatctat ttcaacgata cgcctgcttt ccgcgaaatg gcgatgggtt
     3301 ccgcccttgg caatattatt cccggcttcg aagtgcccaa tgctgtattc atcatgtttg
     3361 gtgcagctat cattgccaac ctgatcctgt ctcgcaccat cctcgggcgc tatacctttg
     3421 ccctgggaag taatgaagaa gccacccgcc tttcgggcgt caatgttgcc ttttggaaga
     3481 ccgcggtcta taccctgtgc ggattgttca gcggcttggg cggcgtgctg atggcttcac
     3541 gcctgaactc ggctcaacct gcccttgggg caggttacga actggacgct attgctgcgg
     3601 ttgtgatcgg tggaacatcc ctcagcggcg gtgaaggttc aattttgggg accatcattg
     3661 gggcgtttat catcagcacg ctcaccaatg gactacgaat cctttcagta cctcaggaat
     3721 ggcagatggt ggtgacaggt ggtattgtca tcctggctgt tttcctggat attctgcgcc
     3781 gccgtcgtta agtagtgttt gcggacggca ttttattcac gacattccat aagaaggagg
     3841 cacaatatca gcgataataa aatgcaaatc caaacagtaa ttaaccttgt tcgaatccaa
     3901 actatctaaa tgaggagatt tatgaacacc aaaaaagtgt tgtttgctct gttaagcgcg
     3961 ctcgtattga tttccgtgat tgctggctgc gctcccgccg ctcaaccagc cgcaccggct
     4021 gagcccgctg caaaacccta tatccccgtg atttccaaag gcttccagca ccagttctgg
     4081 caggcagtga aactgggtgc cgaacaggca gccacagacc tgaatgtgga tatcaccttc
     4141 gaaggtccgg aatccgaagc aatggttgac aagcaggttg aaatgttcca gaccgccctg
     4201 gataagaaac ccgcagctat ttgtctggct gccgttgact ccaaggcctt ccagaacctg
     4261 ctcgaaaaag ccaaggatgc cggcatcccc gttgtcggtt tcgactctgg tgtggatagc
     4321 gatatccccg ttaccaccgc tgcaacagac aacattgccg cggctgcttt ggctgctgac
     4381 aagatggtcg aactgatcgg tggcgaaggc gaagttgccg ttatcgccca cgaccagacc
     4441 agccgcactg gtatcgaccg cgttgccggt ttcgttgacc aggtcaagaa caagtacccg
     4501 aatgtcactg ttgtagacgt ccagtatggt ggcggcgacc agctcaaatc cactgacctg
     4561 gccaaggcca ttatccaggc ccacccggac ctgaagggct tcttcggggc gaacgaaggc
     4621 tcaatcatcg gcgttctgaa cgcggtggct gaactaggca tggaaggcca gatcacggtc
     4681 attggttacg actctggtca gcaacaaatg gacgcgatcc gagctggtac cgaagctggg
     4741 gcgatcaccc agaaccccgt tggcatcggc tacaagtgcg tcgaagccgc cctcaaagcc
     4801 tataatggtg aggagctgcc caagaccatc gataccggat tttactggta cgacaaatcc
     4861 aacatcgacg agccccagat ccaggcagta ctctataagt aagtttatgg ttcttccgaa
     4921 ccttgtgtaa agaacagggg ctgttcaaga atcgttcagc ccctgtttta atacagacaa
     4981 aatggaaata tcgacaccat gattaaaacc ggcattctta atcctcaaat tctgtctcta
     5041 atttcccgtg tgcgtcatac taataccttg gtgatcgcag atcgcggttt cccctattgg
     5101 ccgatgattg aaactgtaga tatctcttta gtggatggga ttccaactgt gctgcaggtt
     5161 tttaaagcgt tgaaatcgaa tttcattat
//
LOCUS       DBNHOH010000979         3994 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_126802, whole genome shotgun sequence.
ACCESSION   DBNHOH010000979 DBNHOH010000000
VERSION     DBNHOH010000979.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_126802"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..751
                     /locus_tag="AC16V7_17730"
     CDS             173..751
                     /locus_tag="AC16V7_17730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR6259021.1"
                     /translation="MSELSDESILARAANGDGEAFGALYERYVSRIYNYIYYRTGNAH
                     DAEDLTARVFYRAMRHISNYNDRGLPISAWLYRIAHNLVANWHRDNSRRKEIPLEESV
                     HGRFDSSHPEHDLLKFEERDRLIRIIRRLSAERQQLLILKFVEHMSNAEIGQIMGRSE
                     GAVKSLYHRTLLALRDEYELLMTDALIESNNE"
     gene            787..1095
                     /locus_tag="AC16V7_17735"
     CDS             787..1095
                     /locus_tag="AC16V7_17735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6259022.1"
                     /translation="MYKMARSFLKGNIKDVRDFEARLEATLKPVRPPEEFVRDLREKL
                     LAQIRQAESGSKRSTKQIILIVAAGFLSVILIIVAGLRTLASFIIGLKLIKRVRKTKS
                     "
     gene            complement(1127..1888)
                     /locus_tag="AC16V7_17740"
     CDS             complement(1127..1888)
                     /locus_tag="AC16V7_17740"
                     /inference="COORDINATES: protein motif:HMM:NF014632.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF166 domain-containing protein"
                     /protein_id="HIR6259023.1"
                     /translation="MRILAIISGDYGTRHVENIRKFGPPSWTINTWQAPAAFPLIIDY
                     PEDYIPQELPETDLVLSFAEHRGVAELLPDIAKMTNAKAVVASIDNESWLPRGLARQL
                     RGWLADNGVACATPKPLCSLTENDFGVTRRQRIKFESPYIAEFATYFGQPSLIIQVDP
                     QSRIIQKAEVRRDAVCGCARYVAERLVGISADDASEKAGLLHHHYPCLASMGKDSDFG
                     DTLMHVSGNVLKDNVEVQVRPYKRIQYISPGTRSE"
     gene            complement(1908..2984)
                     /locus_tag="AC16V7_17745"
     CDS             complement(1908..2984)
                     /locus_tag="AC16V7_17745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6259024.1"
                     /translation="MIQGRFRFPFLFLALLGLIAAMWAGWIRLGWDWPPIQPSLPASH
                     GPLMVVGFLGTLIALERAVALRQRWMYFGPLLSGIGGLATIIGIPGLLGPSLMALGSL
                     VLVAILIVILRQHIALHTVAMALGATSLAIGTILLFIGWPIPRIVLWWEAFLVLTIAG
                     ERLELGRLLKLSKGIVWAFSAVTALSLLGLATSTISLIWGTRIFGLGLLGMAAWLLRY
                     DIARHTIRQNGLARFAALSMLSGYGWLAIGGLLALIYGGQAAGPVYDALLHSVFIGFV
                     MTMIFAHAPIIFPSILGLPISFSRLFYTHLILLHASLLLRIIGDLAMVMPLRRWGGLL
                     NGIAILTFLVITIVSMLTNRERSI"
     gene            complement(2985..3314)
                     /locus_tag="AC16V7_17750"
     CDS             complement(2985..3314)
                     /locus_tag="AC16V7_17750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007339783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR6259025.1"
                     /translation="MSDNYLYVGDLAAQIPELQPDSIISRTIYTDDQIKVILFAFAPG
                     QELSEHTASVPATLHFLEGEAQLILGEDEHEAMPGTWVHMPAKLPHSVYAKALVKMLL
                     IMHTQKA"
     gene            complement(3331..3675)
                     /locus_tag="AC16V7_17755"
     CDS             complement(3331..3675)
                     /locus_tag="AC16V7_17755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6259026.1"
                     /translation="MNSATTWRTLFEKEILQAESARAAGNEGMARVCARRAAGVVAGE
                     YLRRQGLSLSNPSAYARLKFLSQIPQTPPTIQEIVDHFLIKIDFNHSLPVRADLIAEA
                     RWLANELLSWPN"
BASE COUNT         1099 a          896 c          994 g         1005 t
ORIGIN      
        1 cccaccaaaa caccccaaac actctttttg cgggatgccc ctcaaggcat atgtaattgc
       61 cctgtatagc acgcgctttg catatttgtc agcgttgatg ttatactcaa tggtaacttt
      121 attgaaccgg cagggttttt attattaacc ctgctgccac taggtgaaaa ctgtgtctga
      181 actcagcgat gagtcaattc tagccagggc agcaaatggc gatggagaag ccttcggcgc
      241 gctttatgag cgctatgtaa gccggattta caactatatt tattatcgaa cgggcaatgc
      301 tcatgatgcg gaagatttaa cggctagggt attctaccgt gcgatgcgac acatctcaaa
      361 ttataacgat cgtggcttac cgatatctgc gtggctttac cgcattgctc acaatctggt
      421 agcaaactgg catagagata atagtcgccg gaaagaaatt ccccttgaag agagcgtgca
      481 cggtcgattc gatagcagtc atccagaaca tgatctgctt aagtttgagg agcgtgatag
      541 gcttatacgg atcattcgtc gactttcagc agagcgccag caattattga tccttaaatt
      601 tgttgagcat atgtcaaatg cagagatcgg ccaaattatg ggccgctcag agggcgctgt
      661 aaaaagcttg tatcaccgaa ctcttctcgc cttacgggat gaatatgagc ttctgatgac
      721 ggatgcactt attgaaagta ataatgaata acaagatagg tagtgtaacg ttaagaataa
      781 gggtaagtgt ataaaatggc gaggtcgttt ttaaagggca atataaagga tgttcgtgat
      841 tttgaagccc gtctggaggc tacactcaag ccggtgaggc caccagagga gtttgtgcgc
      901 gatcttcgcg agaaattgtt agcgcaaatc cgccaagcag aaagcgggtc taaaagaagt
      961 acaaagcaaa taatcttaat tgtggctgca ggatttttaa gcgtaatctt gattatcgtg
     1021 gctgggttgc gaacgctggc atcctttatt atcggactaa agcttataaa gcgagttcgt
     1081 aagaccaaat cgtaatcagg tagtgttggt ttcttactgg tttcgctcat tcgcttcttg
     1141 tgccggggga aatatattgg atacgtttgt atggtcggac ctggacttca acattgtcct
     1201 tgaggacatt gccagacacg tgcattagag tgtcgccaaa atcgctgtct ttccccatgc
     1261 ttgctagaca tggatagtga tgatgcaaaa gcccagcctt ctcgctagca tcatctgccg
     1321 agatacccac taatctttcg gcaacatagc gcgcgcaacc acatacggca tcgcgacgga
     1381 cctcagcttt ttgaataatg cgagattggg ggtcaacctg aataatgagg gaaggttgac
     1441 caaaataggt tgcgaattcc gcaatatacg gactctcaaa ttttattctt tgtcggcgtg
     1501 tgacgccgaa atcattttct gtcaaggaac agagtggttt tggtgtggcg cacgccaccc
     1561 cgttatctgc cagccaaccc cgtagctgcc gagcaaggcc gcgcggcagc caagattcgt
     1621 tgtctattga ggcaacaacg gctttcgcat ttgtcatctt ggcaatatct ggcaagagct
     1681 cggcaacgcc acgatgctca gcaaatgaca gcaccaagtc agtttcgggc agttcctggg
     1741 gaatatagtc ctccgggtaa tctataatca gaggaaatgc agctggagcc tgccaggtgt
     1801 tgatggtcca ggatggggga ccaaatttgc ggatattttc gacgtgacga gtgccgtaat
     1861 caccagatat aatcgccaaa atgcgcatag acacctcctg ttagatttta tatgcttctt
     1921 tctcgattgg tgagcattga aacaatcgtg atgacgagaa aggtgaggat tgcgatacca
     1981 tttagaagac ctccccagcg gcgcaaaggc attaccattg ctaaatcgcc gataatacgc
     2041 aacaacagcg aagcgtggag taagatcagg tgggtgtaaa acaagcgact aaacgagatg
     2101 ggcaatccca gaatggaggg gaagataatc ggagcgtggg cgaaaatcat tgtcatcaca
     2161 aaaccgataa acaccgagtg caacagggcg tcatacacag gtccagcagc ttgaccgcca
     2221 tatatcagag caagcaggcc gccaatggct aaccatccgt atcctgagag catggaaaga
     2281 gctgcgaatc gagccaagcc attttgccgg atggtatgcc gtgcaatgtc atatctcaac
     2341 agccatgcag ccatgccaag caatcccaag ccaaaaattc ttgttcccca gataagcgaa
     2401 attgttgagg ttgccaagcc cagcagagac agggcggtga ctgctgaaaa tgcccataca
     2461 ataccttttg agagttttag taagcggcct aattccagac gctctccggc gatggttaaa
     2521 acgagaaaag cctcccacca gagcacaatt cgaggaatcg gccaaccaat aaacaaaagg
     2581 atggtgccaa tagctaaact tgtcgcaccc aatgccattg caaccgtatg caacgctata
     2641 tgctggcgta aaataacaat gagaattgcg acaagaacca ggctgcccag cgccatcaag
     2701 gatggtccta gaagtcctgg aatgccaatg atcgttgcta atccacctat accgcttagg
     2761 agtggtccaa agtacatcca tctttggcga agggcaactg cacgctccaa ggcgatcaga
     2821 gttcccagga agcctactac catcaatggg ccgtgtgaag cgggaagaga tggctggatg
     2881 ggtggccaat cccagcccag gcgaatccag cctgcccaca tagcagcaat caatcccagg
     2941 agagcgagaa aaaggaaggg gaatcgaaat cgaccttgga tcatttacgc tttttgggtg
     3001 tgcatgatta atagcatctt gactaaagct ttcgcataga cactatgagg taacttcgca
     3061 ggcatgtgta cccaggttcc tggcatggct tcatgctcgt cttcaccaag aattagttga
     3121 gcttcccctt ctagaaagtg taatgtcgct gggacagagg ctgtatgttc agagagttcc
     3181 tgtcctgggg caaatgcaaa tagaattact tttatctgat catcggtata gattgttcgg
     3241 cttatgatgg aatcgggctg taattcaggt atctgggcag ccagatcacc tacatagaga
     3301 tagttgtcgc tcataatatg ttgtcctttt tcaatttggc catgaaagca attcattcgc
     3361 aagccaacgc gcctcagcaa ttaggtcggc gcgaacgggt aagctgtgat tgaaatcaat
     3421 tttgatcaag aaatgatcga caatttcctg aattgtgggt ggtgtctggg gtatctggct
     3481 gagaaatttc agccgtgcat acgcgctggg gttcgaaagt gataatccct gtcggcgcag
     3541 atattcccct gcaactaccc cagccgcgcg gcgggcgcaa actcgtgcca taccttcatt
     3601 acctgccgca cgtgcagact cagcctggag aatctctttc tcaaaaagcg ttcgccaggt
     3661 ggttgcactg ttcataaaca aattgtaccg taaaagtcga ttactcaaaa tgttataatc
     3721 gatctaaatg attcgagatt tgcgtagata tgctcgccag acacagattc gactgatttt
     3781 gggtggcatc ttgctattgt tccttgtagg tgatgggctg atctatatat tctacggaaa
     3841 agaagcagca attttagcga ttttttgcct tgggttaggt ttatctccgc tagtcttgat
     3901 cgggcttata ttatttattc tagattgggc agtgcgacga gcaaacacat gaacggcgat
     3961 ttacaaactc aggttattca cgttggagac tggg
//
LOCUS       DBNHOH010000980         2085 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_62045, whole genome shotgun sequence.
ACCESSION   DBNHOH010000980 DBNHOH010000000
VERSION     DBNHOH010000980.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_62045"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..>2085
                     /locus_tag="AC16V7_17760"
     CDS             122..>2085
                     /locus_tag="AC16V7_17760"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR6259027.1"
                     /translation="MPTALVTHWLFSSIMHHCKCALLLALTILSLSPCAVAAPDRSNQ
                     PLRVAIDDNYPPYIFRNEEGQPQGYLVDLWALWEQQTGVTVELLASDWDLAQERMRTQ
                     QADVIDTVFITPERLKSLDFSPPYAKIPVAIYTHHSIGGIAKLEDLRGFLVGVKAGDA
                     CVEPLKQAGLSTLQYFPNYEALVQGAIDDRVRILCLDEPPANYLFYKHNAESTFNRAL
                     RLEGGEFHRAVHKGEAATLALVNRGFAAIDDPTIQVLVNKWMGNRLSQPSYARHLTYV
                     LPAIALLAALLLLWGATLRHQVRQRTAELNAERNQLRLLIETIPDLVWMKDNSGVFRY
                     CNPQFERLFNTPEAQIVGKTDYDFVDSQRAASFRAHDQQAVKTDSPYVYEEWLTFADN
                     GQRMLVETTKTPVRSPDGRLNGVLGIARDITGRKLAEQALQVSEHRLHTILDSVDAFI
                     YIKDRDYRYQYANRRVCELFGRSMEEIVGHDDSHFFDADTAANLRQNDSRVIDQGERV
                     EAEEVNTNLRDQITSAYHSVKMPLRDQQGQIYALCGISTDITARKQAETSLRIAAAAF
                     EAQQGTLISDANNRILRVNQAFLEMSGYSEAEVIGQDPRMFASGRHDQAFYTTMWDEI
                     INHGSWQGEIWDRRKDGQVYPKWLSIPAVK"
BASE COUNT          442 a          668 c          588 g          387 t
ORIGIN      
        1 ggctgaagcc caaaccctaa cgccaccgtc tgcttgtcac ggccaaggtt cgcgctaata
       61 ggcagcccgc atggattgcg gtacagtgcg attttcctta tgagcaaaac ggttatcgcg
      121 catgcctaca gcgctggtta ctcactggct gttttcctcc atcatgcatc actgcaagtg
      181 cgcactgctg ctggctctga ctatcctcag cctcagcccg tgcgccgtcg ccgcacccga
      241 tcgcagcaac cagccactaa gggtggccat cgacgacaac tacccgcctt atatctttcg
      301 taacgaagag ggccaacccc aaggctacct ggtggatctc tgggccctgt gggagcagca
      361 gaccggcgtc acggtcgagc tattggccag cgactgggat ctggctcagg agcgaatgag
      421 gacgcagcaa gcagacgtca tcgataccgt ttttatcacc cctgaacgtc tgaaaagcct
      481 cgatttctcc cccccttatg ccaagattcc cgtggctatc tacacccacc acagtatcgg
      541 cggcatcgcc aagctggagg atctgcgcgg ttttctggtg ggggtcaagg caggcgatgc
      601 ctgcgtcgag cccctcaagc aagcggggtt gagcacgctg cagtattttc ccaattacga
      661 ggccctggtg cagggcgcca tcgacgatcg ggtgcgcatc ctgtgcctcg atgaaccgcc
      721 cgccaactat ctgttctaca agcacaatgc cgaaagcacc ttcaatcggg ccctgcgcct
      781 ggaaggaggg gaatttcatc gtgccgtgca caaaggggag gcggccaccc tggcgctggt
      841 caatcgcggc tttgccgcca tcgacgaccc cacgatccag gtgctggtca acaagtggat
      901 gggcaaccgc ctgtcccagc ccagctacgc ccgccacctc acctatgtgc tgcctgccat
      961 cgccctgctt gcggcgctgc tgttgctatg gggcgctacc ctgcgccacc aggttcgcca
     1021 acgtactgcc gagctgaacg ccgagcggaa ccagctgcgc ctgctgatcg aaaccatccc
     1081 cgatctggtg tggatgaagg acaacagcgg ggtgttccgc tactgcaatc cgcagtttga
     1141 gcgcctgttt aacacccctg aagcgcagat cgtcggcaag accgattacg atttcgttga
     1201 cagtcaacgc gctgccagct ttcgcgccca cgaccaacag gcggtcaaga ccgatagccc
     1261 ctacgtctac gaggagtggc tgacctttgc cgataacggc cagcggatgt tggtggagac
     1321 caccaagacc ccggtacgca gcccggacgg tcgcttaaac ggcgtgctgg ggatcgcgcg
     1381 tgatatcacc gggcgtaaac tggccgagca agcgctgcag gtgagcgagc atcggttgca
     1441 caccatcctc gacagcgtcg atgcctttat ctacatcaaa gaccgcgact atcgctatca
     1501 atacgccaac cgccgcgtgt gcgaactgtt tggccgatcg atggaggaga ttgtcggcca
     1561 cgacgacagc cattttttcg atgccgacac cgctgctaac ctgcgccaga acgatagccg
     1621 cgtgatcgac cagggggagc gtgtagaggc agaagaggtc aataccaacc tgcgcgacca
     1681 gatcaccagc gcctaccatt cggtcaagat gccactgcgc gatcagcagg gccagatcta
     1741 cgccctgtgc gggatctcca ccgatatcac cgcccgcaag caggccgaaa cctcgctgcg
     1801 tatcgctgcc gccgcgtttg aagcccagca ggggaccctg atctccgacg ccaataaccg
     1861 catcctgcgg gtcaaccaag cgtttctgga gatgagcggt tacagcgaag ccgaggtgat
     1921 cggccaagac ccgcgcatgt tcgcctccgg ccgccacgac caagcgttct acaccaccat
     1981 gtgggacgaa attatcaacc acggcagttg gcagggtgag atctgggacc ggcgcaagga
     2041 tggccaggtc taccccaagt ggctcagtat tcccgcggta aaaaa
//
LOCUS       DBNHOH010000981         1837 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_255078, whole genome shotgun sequence.
ACCESSION   DBNHOH010000981 DBNHOH010000000
VERSION     DBNHOH010000981.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_255078"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..>1837)
                     /locus_tag="AC16V7_17765"
     CDS             complement(53..>1837)
                     /locus_tag="AC16V7_17765"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter family
                     protein"
                     /protein_id="HIR6259028.1"
                     /translation="PIVVGEILAGILIGRSGLGWVNGEDPVLEILSEFGFVFLMFLSG
                     MEIDFSSLGNLPNSNTPRASENKGWGPIQIGGLSFLLTLALSVVIGFVLVQLGLARNL
                     WMVALIMSTTSLGIVLPVLKERGLSVGRYGQTILVSALIADFATMLLITILVAVVSHG
                     LNLEILLVGVLFVVFGIMYRLGVQFFNRLPAIRKVINDLSHATSQFKVRAAFTMMLIF
                     VALSEFLGVEIILGAFLAGAIISVLRTPEDKDLTHQLEAVGFGFFIPIFFIKIGAGFN
                     LGAIIESPQYLLLAPLLLLAALIVKFVPSLLFTRIFSKREALAAGALLSSRLSLIIAA
                     SAIGLRLGLISEAMNAEILLVAVVTVTLGPLLFTRLLPPSEQIEPQPVIVVGAGQLGL
                     EVANRIRTHNEPVYLVDLDQDQVSAAIRRGFEAQVGDLENPDKTLVRKLDQAKALICT
                     YTDPELSYKVCSLARSVFGIDAVVADVPSPNDIPRFEQLDVTPVNAALDRATLLELHV
                     RNPDMYMLLTRLKDSKEVVEIDCNNPACAGKALRQLSFPGDVLALALRRHGELIVPHG
                     DTRIELGDHLTLVGSQDCIDEARSLFAI"
BASE COUNT          493 a          479 c          478 g          387 t
ORIGIN      
        1 ctcgtgaggg gttacttgaa agcactgtgc cctatggatt tggggtgaga tgctagattg
       61 caaaaagcga gcgagcttcg tcgatgcagt cttgagagcc aaccaatgtc aggtgatcgc
      121 caagctcgat gcgcgtgtca ccatgtggca cgatcaattc tccatgcctt cgcagtgcga
      181 gcgcaagcac atcccccggg aagctaagtt ggcgcaaggc ttttccggcg caggccggat
      241 tattacaatc gatttctaca acttctttgc tgtccttcag ccgggtgagc agcatgtaca
      301 tatccggatt gcggacatgc agttctaata gcgtagcgcg atccagggcg gcatttacgg
      361 gtgtgacatc cagctgttca aagcggggaa tatcgtttgg actgggcaca tcggcaacca
      421 ctgcgtcaat tccgaagact gaccgggcca gtgagcacac cttataacta agttcagggt
      481 cagtataggt acaaattaat gccttggctt gatccaactt tctaaccagg gttttgtctg
      541 ggttttctag atcacctacc tgagcctcga agccgcggcg gatggcggca gacacttggt
      601 cctggtcaag atcgaccagg tatacgggct cgttgtgggt gcgtatccgg ttagctacct
      661 ccagtcctag ctgtccagca ccgaccacaa taacgggctg tggttcgatc tgctccgagg
      721 gcggaagtaa ccgcgtgaat aacagcggtc ctagcgtgac ggttacaacc gcaaccagca
      781 aaatttctgc attcatggct tcactgatca agcccaaacg caggccgatc gccgaggcgg
      841 cgataattaa agatagacgc gatgataaca aggcaccggc ggcgagtgcc tcccgcttcg
      901 agaatatgcg agtgaacagc agagagggaa caaacttgac aatcaacgct gccagcagaa
      961 gcaggggtgc caggagcagg tattgggggg actcgatgat cgcacccagg ttaaatcctg
     1021 cgccgatctt gataaagaag atggggataa agaatccaaa accgactgcc tccaattgat
     1081 gggtcagatc tttatcctct ggtgtgcgca gcacagagat aattgcccct gccaggaaag
     1141 cgcccaggat aatctccacc cctagaaatt cagacagggc aacgaaaatc agcatcattg
     1201 tgaatgctgc ccgaacctta aattgggatg tggcgtggct caggtcgttg atcacctttc
     1261 ttatcgccgg caagcgattg aaaaactgaa cgcctaggcg gtacattatc ccaaagacaa
     1321 cgaataatac accgaccaga aggatttcca gatttaatcc atgcgagact acagctacca
     1381 ggatggtgat caagagcatg gtggcaaaat cggcaatcag cgcagagaca aggatcgttt
     1441 gcccgtaacg tccaacactt aagccacgtt ccttcagcac tggcaaaaca atgcccaggg
     1501 aggtagttga catgatgagt gctaccatcc acaggttgcg cgccagcccc agttgaacaa
     1561 ggacaaaccc gatgaccaca gagagcgcca aggttaaaag gaagctcagg ccgccgattt
     1621 ggataggccc ccaacccttg ttctccgatg ccctcggggt atttgaattt ggtagattgc
     1681 ccaggctgga aaaatcaatt tccattccag aaagaaacat gaggaagacg aaaccaaatt
     1741 cactcaagat ttccagcact ggatcttcac cgttcaccca acccaatcca ctgcgcccga
     1801 tcagaatacc cgccagtatt tcaccgacga caattgg
//
LOCUS       DBNHOH010000982         3288 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1729665, whole genome shotgun sequence.
ACCESSION   DBNHOH010000982 DBNHOH010000000
VERSION     DBNHOH010000982.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1729665"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="AC16V7_17770"
     CDS             complement(<1..230)
                     /locus_tag="AC16V7_17770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6259029.1"
                     /translation="MNASLPDRLTLAAFSVVVLLGGLNAVAVRFSNQELPPFWGAALG
                     FAFASVSLLLIVYFQPAVAITRGAGSGRGRSL"
     gene            complement(255..1763)
                     /locus_tag="AC16V7_17775"
     CDS             complement(255..1763)
                     /locus_tag="AC16V7_17775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005251153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR6259030.1"
                     /translation="MGISLPPDVALHPSTVEFINSGPKKLLIGGEWVDAASGETFATI
                     NPATGEELTQLALAGPQDVDRAVKAARQAYEHGPWSKFSGEERGHLLWKVADLIEAHA
                     DELAELDTLDNGKPLRVARRGDLPSAARHFRYYAGLAGKIEGSTIPVSMPNQFVYTRR
                     EPMGVVGLIIPWNFPLLMCAWKLAPALACGNTALLKPAEETPLSALRLGDLIEEAGIP
                     PGVVNILTGPGLPTGAAIVAHNDVDKVAFTGSTQVGREIMKNAAASNLKRISLELGGK
                     SPNVIFADADLSKAIRGANWAIFSTSGQECVAGSRLFVERPVYEQVIEGLAAEAQKIR
                     VGNGFTPKVHIGPIISEQQLQRVLGYIASGRSGGAQVVTGGQRLGGELESGYFLSPTI
                     FTHQDDTLSIVQEEIFGPVVAATPFDEWDNLVQRANMTHYGLAAGVWTRDISKAHRFA
                     HAMKAGTVWINGYGHFDAAAPFGGYKESGFGREMGTDAIELYTQVKTVWVGL"
     gene            complement(1801..2691)
                     /locus_tag="AC16V7_17780"
     CDS             complement(1801..2691)
                     /locus_tag="AC16V7_17780"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR6259031.1"
                     /translation="MNNRRYTGKHPSEFGFEAIQYEKKEYRATITINRPQSLNAVNFQ
                     VLSEMNTALKDASWDDGVAVLVLTGAGERAFCTGADLKEQRQFLKRPRDYWKWMGEFI
                     ECHERLRNLGKPSLARLNGIVVGGGNEFNLSCDLAVAADDIYIRQVGTSHGSVALAGA
                     TQFLPLVVGERRAREILLLNEEIPAHKALEWGLVNWVVSRADLDDKVDEIAQKLVDKF
                     PEKTRYTKQQLNFWRDLSWHLTIGHGRDWLSTHNTSPETWEGVRAFNQKRKPDYQSIR
                     RRWVEDQAPEWLDGETPQND"
BASE COUNT          746 a          913 c          827 g          802 t
ORIGIN      
        1 catagtgaac ggcccctacc agagcctgcc ccgcgggtaa tggcaaccgc tggctggaaa
       61 tatacgatca agagaagcga tacagatgcg aatgcaaacc cgagggctgc gccccagaat
      121 gggggaagtt cctgattgct gaaacggacc gcaaccgcgt tcaagcctcc aagaagcacc
      181 actactgaaa aagcggctag tgtcaggcga tctggtaggc tggcgttcat ctatatacct
      241 atctataaac ctatctataa accaacccaa actgttttaa cctgcgtata caactcaatc
      301 gcatctgtgc ccatctcgcg gccaaaacca ctctctttgt agcctccaaa gggcgcagcg
      361 gcgtcgaaat gaccatagcc gttaatccat accgtgccag ccttcatggc gtgagcaaat
      421 cgatgggctt tgctgatatc ccgcgtccaa actccggcag ccagcccgta gtgggtcata
      481 ttggcgcgct gcaccagatt atcccattca tcaaagggtg tggcagctac cactggacca
      541 aagatctctt cctgtacgat ggatagggtg tcgtcctgat gggtaaaaat ggtaggggaa
      601 aggaaatatc cactttccaa ttctccaccc aggcgttgcc cacctgtaac tacctgggct
      661 ccaccgctgc gcccgctggc gatatacccc aagactcgct ggagttgctg ctcggaaatt
      721 attgggccga tatgtacttt gggagtaaat ccattgccca cacgaatctt ctgtgcctcg
      781 gctgccaatc cttctattac ctgctcgtac accgggcgct cgacgaagag tcgcgaacca
      841 gccacacatt cctgcccgct cgtgctgaaa atcgcccagt ttgcgccacg gatagccttg
      901 ctcaggtcgg cgtcggcaaa aatcacgttt ggcgattttc cacccagctc aaggctgatc
      961 cgcttcaagt tgcttgcagc ggcattcttc atgatctcgc gacccacttg agtggagccg
     1021 gtgaaagcta ctttatcgac gtcattgtgg gccacaatcg cagccccggt gggcaagccg
     1081 ggtccagtca gtatattgac cactcctgga gggatgccag cttcttcaat tagatcgccc
     1141 agccgcaagg cagacagcgg agtttcttcg gctggcttga gcagggcggt gttgccacaa
     1201 gcgagtgcgg gggcaagctt ccaggcgcac atgagcaacg ggaagttcca tggaataatc
     1261 agccccacca cacccatcgg ctcgcgacgg gtatatacga actggtttgg catgcttact
     1321 gggattgtgc tgccttcgat cttgccagcc aaaccagcat aatagcgaaa atggcgggca
     1381 gcggagggga ggtctcctct gcgcgccact cgcaggggtt tgccattgtc taacgtatcc
     1441 agctctgcca gttcgtcggc gtgtgcctcg atcaggtctg ccactttcca gagcaggtga
     1501 ccgcgctcct ctccgctaaa tttgctccat ggaccgtgct cataagcctg gcgggcagcc
     1561 ttcacggcgc gatccacatc ttgcgggccg gctaatgcca attgggttag ctcctccccg
     1621 gtagcggggt taatcgttgc aaaggtctcc ccgctggcgg catccaccca ttcaccacca
     1681 atcaagagct tctttgggcc agagttgata aattctacgg tcgaagggtg taaagcgaca
     1741 tctggcggta agctaatccc cataagaacc tcttttcttc agtcaattct attattacga
     1801 tcaatcgttt tgcggtgttt ctccgtctag ccattcagga gcttggtctt ctacccagcg
     1861 acggcggata gattggtagt cgggcttgcg cttttgatta aaagcacgca cgccttccca
     1921 agtctctgga ctggtgttgt gagttgagag ccagtcccgc ccgtgaccga tggtgagatg
     1981 ccagctcagg tctcgccaaa agttaagctg ctgcttagta tagcgggttt tctctgggaa
     2041 tttatccacc agcttttggg cgatctcatc aactttatca tctagatccg cacgcgacac
     2101 cacccagttt accagacccc attccagcgc tttgtgggca ggtatttctt cgttcagcag
     2161 gagaatctcc cgcgccctgc gctcgcccac aaccaggggg aggaattggg ttgccccagc
     2221 cagagcaacg ctcccgtggc ttgtacctac ctgacggatg tatatatcgt ctgcagcaac
     2281 cgccagatca caactcaagt tgaattcatt gccacctccc accacgatcc cgttcaggcg
     2341 ggcgaggctg ggcttaccca gattgcgcaa gcgctcatgg cattcgatga actcgcccat
     2401 ccatttccaa taatcccgtg ggcgttttaa gaattggcgc tgctccttca ggtcggctcc
     2461 ggtacagaag gcgcgctcgc ctgcacctgt gagtaccagg acggcaactc catcgtccca
     2521 gctggcgtct tttagagcgg tgttcatctc gctcaacacc tgaaaattga ccgcgtttag
     2581 agactgcggg cggtttatgg tgatggtagc gcgatattcc ttcttctcat actgtatggc
     2641 ttcaaagcca aactcggagg gatgttttcc agtgtaacgt cgattattca tttgaactct
     2701 tgaagatatt gttatatagc agggcagcca ccgccgcccc taaaacaggt ctaacaaagt
     2761 aaacccatag gttaggttgg agaaattggc tgtagccagc gctcaggtct atcgtgatcg
     2821 tttttcatgc gtttctcttt agaggggcaa tgaattgtct tgatcccaag cattgatcta
     2881 cgccgggttc tcaaattata ttatcacaaa atgggtgaat atagaatgaa attaacgagt
     2941 gaagcggaga taacaatcac ataatggagc gctggatgtt tccttgttct cgagaggaaa
     3001 ctatttgaga cctctccctc cataggcatc catccgggtt cgccatattc gccgttatgg
     3061 cggcgaagct ccttctgagt gtattcacgc ttgattacag acaatctcag taaccttgat
     3121 aatcgaatga agcccgatgt gtggtcggct tcgctaaact tgagtgcgcc aaatacccat
     3181 ttaacagcga atgtgtacac agtgtgttat agcgtgcatt gtgtccacaa atagggcgaa
     3241 aaagtgtccg ttgtgtccat tatctccatt gtttccaccg cttctgca
//
LOCUS       DBNHOH010000983         1253 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_511815, whole genome shotgun sequence.
ACCESSION   DBNHOH010000983 DBNHOH010000000
VERSION     DBNHOH010000983.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1253)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_511815"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="AC16V7_17785"
     CDS             <1..369
                     /locus_tag="AC16V7_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR6259032.1"
                     /translation="LFIAHDLSVVEHICNRVGVMYLGNLFEMADTQELFHHPLHPYTE
                     ALMSAIPEADPSIVMQPIKLTGEIPSPIDPPSGCSFHPRCQYAQEVCSTTKPEWVEQQ
                     PEHFVACHFADSLQLKGFAG"
     gene            complement(381..1124)
                     /locus_tag="AC16V7_17790"
     CDS             complement(381..1124)
                     /locus_tag="AC16V7_17790"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015000.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIR6259033.1"
                     /translation="MDICLFGHRGAAGEAPENTLGGFAYAWGIGVRAFELDIHLSKDE
                     QLVVIHDPTLERTTDGVGLVSDYTAAELSSLNAACRFPGWSNREGVPSLQNVLARYHT
                     GIHRWQLEIKTDAPRRLEVVCKILARQIDHFDIYERVKVTSFDPAALQIMRRTAPHLS
                     LGLIRSPMLLEDIDLAQTLGCSEVCMPVESGGAWLVSAAHEAGLEVTGWLGNNPTQME
                     RLLAWGVDAITTDVPSFAQQYMNNRSNLE"
BASE COUNT          286 a          364 c          315 g          288 t
ORIGIN      
        1 ctgttcatcg cccacgacct gagcgtggtg gaacacatct gcaaccgggt gggtgtgatg
       61 tacctgggga acctgttcga aatggcagat acacaggaac tgttccacca tcccctgcac
      121 ccctacaccg aagccctcat gtccgctatt cccgaggctg accccagtat tgtgatgcag
      181 cctattaagc tcactggtga aatacccagc cctattgatc caccaagcgg ctgttccttc
      241 catccccgct gccagtatgc ccaggaggtc tgctcaacga ccaagccgga gtgggtagag
      301 cagcagccgg agcatttcgt cgcctgccac ttcgccgatt ctctgcagct caagggattt
      361 gcaggctgat ctcatctcct ttattctaaa ttcgatcgat tgttcatata ttgctgtgca
      421 aatgagggca catctgttgt tatggcatct acgccccaag ccagtaagcg ttccatctgt
      481 gttgggttgt tgcctagcca gccggttacc tccaagcccg cctcgtgagc agcggaaacc
      541 agccaagcac cgccgctttc cacaggcata catacttctg aacaacccag ggtttgcgcg
      601 agatcaatat cctctaatag catcgggctg cggatcagac ctaggcttag atgaggagca
      661 gtacgacgca tgatttggag cgctgccggg tcgaatgaag tcactttaac cctctcgtag
      721 atatcaaaat gatcgatttg ccgggcaaga atcttgcaaa cgacctccag gcgcctggga
      781 gcgtcggtct tgatctccaa ctgccagcgg tgaatgcctg tgtgatagcg cgccaggacg
      841 ttttgcaatg aaggcacacc ctcccgattt gaccaaccgg gaaagcggca ggcagcgttc
      901 agggaagata attccgcggc ggtatagtca cttactagcc caacgccatc tgtagtgcgt
      961 tccagggttg ggtcgtggat gactaccagt tgttcatctt tggacaggtg aatatccagc
     1021 tcaaaagccc gaacgccgat cccccatgca tacgcaaaac ctcccagtgt gttttcaggt
     1081 gcctcgccgg ctgccccgcg gtgtccaaac aggcaaatat ccaagtcgtt ttactcctca
     1141 aagctgtgat ctttcacaaa ctggatagaa actcgcaggt tcttaacagg gttccggctt
     1201 tgaccgccag cgggattaca gggttccatc tcccccaaag aaatcgtcca ggg
//
LOCUS       DBNHOH010000984         5966 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_575072, whole genome shotgun sequence.
ACCESSION   DBNHOH010000984 DBNHOH010000000
VERSION     DBNHOH010000984.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5966)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5966)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5966
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_575072"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="AC16V7_17795"
     CDS             complement(<1..459)
                     /locus_tag="AC16V7_17795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR6259034.1"
                     /translation="MSPKSGHIGSIVAWIVALSLVLFAAYLGWQMVPSKATVPVQNAS
                     AETIAFLQEPLDPMVSLPKYTHAKSSHALVRKVVPRTVIPSRPSLEVKSYTVELGDSV
                     FEIASNFKIKPETLLWANYDQLNDNPDMVSIGMSLNIPPIDGVYYQWQDGD"
     gene            complement(498..1778)
                     /gene="rho"
                     /locus_tag="AC16V7_17800"
     CDS             complement(498..1778)
                     /gene="rho"
                     /locus_tag="AC16V7_17800"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR6259035.1"
                     /translation="MKVIELENIPLNALRQKAKELDVPNALRLKKEDLIMRIQQTEAE
                     REGLEVRGGILEIMSEGIGFLRSAHYQPGPYDVYVSQSQIRRYNLRNGDLIIGNVRPP
                     RESEKHYGLLKVESVNGLDPEIARHRPAFESLTPIFPDTRFDLELDRHTLATRLINLV
                     TPIGRGQRGLIVSPPKAGKTTILKQCANAISNKYPEVHLMVALIGERPEEVTDMDRSV
                     DAEVISSTFDEPVTSHVRVAEMALERAKRLVEVNRDVVILMDSITRLARAYNLVVTPS
                     GRTLSGGIDPSALYPPKRFFGAARNIEEGGSLTIIATCLVDTGSRMDDVVYEEFKGTG
                     NMELHLSRRLQERRVFPAIDIERSSTRREELLLGPDITPRVWLMRRMYDQMVTAPPHG
                     AGLDPSTATEAILQRLARTSNNLEFLETLTEDNR"
     gene            complement(2291..4690)
                     /locus_tag="AC16V7_17805"
     CDS             complement(2291..4690)
                     /locus_tag="AC16V7_17805"
                     /inference="COORDINATES: protein motif:HMM:NF025141.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HIR6259036.1"
                     /translation="MKFKLALMLLFVFTLAALPTSARADGIIIPDPPICLDGPCPPPP
                     CPRPFPCPPRPSFVQLAIRYHRVSVTIQDQLVVTHVDQVFHNPNDWQVEGTYIFPIPV
                     DAAVSSFSLWIDGKPVEGKVLDAEEARKTYEEIVRSMSDPALLEYVDRGAVQARIFPI
                     PPGGERRIELEYVQALTAENGLVRYTYPLSTEKFSLWPLENVSISVEVRSSQAPIRAV
                     YSPSHEIAINRLDDRHVMAGYEDVDVLPDTDFTLYYSLGDEQAFHLLTYRDRDDPVDP
                     DGFFLIMLAPRPEAAIETLSKDVILVLDRSGSMDGEKFRQAQEALRYILRHLNPDDRF
                     NIIVFSTGLETYGRNLRPAEEANEAIAWVDRQSAQGSTDINRALLEAAAMSDRERPTY
                     LIFLTDGLPTEGIVDSQDILDNLDTTAPDNLRLFAFGVGYDVDTFLLDSLAQAHHGSS
                     SYVLPGERLDEVLSAFYAKISSPVLTDLSLDFEEIRTYDIYPDPLPDLFAGSQIIVVG
                     RYREGGIANIVLRGKVEGRRQTFRFDEQRFADDDDEIRSSVLDSIPRLWATRKIGYLL
                     NQIRLNGPDKETIDQIVRLSIRYGIVTPYTSYLVTEEMPLGEAEQERIAREQYNQLSQ
                     MAPAPSSGQGAVERAAEQGGMAAADSAAPPSEEAAQTVRIVGSKTFVVTNGVWVDTGY
                     DPMAMQTVEVAFLSDDYFALVASRPKLAAAFALGPRVIALEEGIAYEVVEGTTEVKPI
                     KIPPTHTPVPQPTGTPFPTLEPVIEITAEVTPTAPGTMPCASGLLPLGLLVIWLRRKR
                     E"
     gene            4900..>5966
                     /gene="prfA"
                     /locus_tag="AC16V7_17810"
     CDS             4900..>5966
                     /gene="prfA"
                     /locus_tag="AC16V7_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR6259037.1"
                     /translation="MLEKLAGIESRYQELNQLLNEVGDDYQRAAELNKERIDLEPLYS
                     KAQEYRKSLDQLEQARHLLDTDDEELRSLAETEFTELEELIFQLEDEIKAMLLPKDRR
                     DQRNVIVEIRAGTGGEEAALFASDLFRMYSRYAERRRWDVDILSANETGIGGFKEIIF
                     MVKGRSAYSRLKYESGVHRVQRIPVTEASGRIHTSTATVAVLAEVDEVEIDIPDSDIR
                     MDVFKSAGAGGQNVQKNATAVRITHIPTGIVVACQDERSQLQNRMRAMSILRARLYEI
                     EEEKRRLEVDETRRSQVGTGERAEKIRTYNYPQSRVTDHRINISNFNLPMFIDGDIDE
                     FIDELATRDEAARLAAVGLEI"
BASE COUNT         1540 a         1550 c         1542 g         1334 t
ORIGIN      
        1 atccccgtcc tgccactggt aatacacgcc atcaattggt gggatattca agctcatccc
       61 aatcgaaacc atatccggat tatcgtttag ttggtcgtaa tttgcccaga gtaacgtttc
      121 gggcttaatc ttgaaattgc tcgcaatttc aaacaccgaa tcccccaact caacggtgta
      181 cgatttgacc tctaagcttg gacggcttgg gataacggtg cggggaacca ctttccgcac
      241 aagtgcatgg gaggacttag catgagtgta ttttggaagt gaaaccatcg gatcaagcgg
      301 ttcttgcagg aatgcaatgg tttcagctga agcattttgc accggtactg ttgccttact
      361 gggaaccatt tgccaaccta aataagcggc aaacagcacc aggctcaatg caacgatcca
      421 ggcaacaatg gaacctatat gaccagattt gggggacatg aaagggctac cgcggtaaga
      481 ccgcagatct tagcagatta tcgattgtct tcagtcagtg tttccaagaa ctccaggttg
      541 ttggaagttc gtgccagacg ctgcaagata gcttcggtag ctgtcgaggg atctaatcct
      601 gcgccgtgcg ggggtgcggt gaccatctgg tcgtacatcc gccgcattaa ccaaacgcgc
      661 ggagtgatat cggggcctaa gagtaattcc tcgcgccgtg tagaagagcg ctcgatgtcg
      721 atagccggaa aaactcgccg ctcctgaagg cgccgggaca agtgcaactc catattaccc
      781 gtgcctttga actcctcgta caccacatca tccatgcgcg agccggtatc gaccagacat
      841 gtagcgataa tcgtcaaaga tccaccctct tcaatattgc gggcagcgcc aaaaaagcgc
      901 ttaggcgggt aaagcgccga aggatcgata ccgccagaca atgtacggcc tgaaggggta
      961 acgactaggt tataagcccg cgccagccgg gtaatcgagt ccatcaaaat gaccacatcc
     1021 cgatttactt ccacgagccg cttggcgcgt tccaaggcca tttccgccac acgcacatgc
     1081 gaggtaacag gctcatcgaa tgtagacgag atcacctcag catccaccga acggtccatg
     1141 tcagtgacct cttcggggcg ctcaccgatt aacgccacca tcaaatgcac ctcaggatac
     1201 ttatttgaga tagcattagc acattgcttt agaatggtgg ttttgcctgc tttaggcggc
     1261 gaaacaatga ggccacgctg cccacgcccg atgggagtca ccagattgat cagacgggtg
     1321 gccagggtat ggcgatctaa ctccagatca aagcgagtgt ccgggaaaat cggggtgagc
     1381 gattcgaaag ccggtctatg gcgagcgatc tcagggtcca gtccgttgac actctcgact
     1441 ttcagcaagc cgtaatgttt ttcggattcg cgcgggggtc tgacattacc gatgattaaa
     1501 tcaccatttc gcagattata gcggcggatc tgggattgtg agacatacac gtcgtatgga
     1561 cctggttgat aatgagcaga gcgcagaaaa ccgatgccct cactcatgat ctccagtatg
     1621 ccaccacgga cttccagacc ttcacgttcc gcttcagtct gctggatccg catgatgaga
     1681 tcttcttttt tcaaacgcaa cgcatttggg acgtccaact ctttcgcctt ctggcgaaga
     1741 gcattcaggg ggatattttc taattctatg accttcattt ttagtgactc gctttaatat
     1801 ctaatagcat ggattaggta ccaccgccag gactagctca cttgccggca tactagacgg
     1861 ggggccaata agcacaatgg caaaatcaaa ggggaagcat taagataaaa ggagaaattg
     1921 cgtaggaatt atagcatgca tggtaattac ccgcaagtat ctcagataac caaatttgac
     1981 aaaaaaagga atctccagtc aaacggcaag tgccactaac gcaatttgct ttcgcttata
     2041 tatgcaagcc ccgatttaat atactagtac ggaatcacgc cccagaggta tattcattat
     2101 acactaattc tctttgcgcg caagtcgcag ggggcaaaaa tttcaagatt gttagacggc
     2161 ttaatcactc ttgtttctaa cctgctcaat tcattagata ggcaaattcg aattgaatag
     2221 gaagcgaaac acgaactagg gtgtcttgat aaaacataac accctagtta atatccttca
     2281 gcgagaaaaa ctactccctt tttcgcctta gccagatgac gagcaatcct agaggcaata
     2341 atccactggc gcagggcatg gtgccaggag cagtgggtgt gacctccgcc gtaatttcaa
     2401 tcacaggctc taacgttggg aagggcgtac ccgttggttg cgggacaggc gtatgggttg
     2461 gcgggatttt gatgggcttc acctctgtag tgccttccac aacttcgtaa gcaattcctt
     2521 cttccaatgc gatcactctc ggccccagag caaatgccgc cgccagtttt ggacgagagg
     2581 ccaccagggc aaaataatcg tcagatagga atgccacttc aactgtttgc attgccattg
     2641 gatcataacc ggtatccacc cagacgccgt tggtaaccac aaacgtcttc gagccgacta
     2701 tgcgcacagt ctgtgctgct tcttccgacg gtggtgcagc cgagtcggct gcggccatac
     2761 cgccctgttc ggcagctctc tcgaccgccc cctggccact ggacggcgca ggcgccatct
     2821 gagatagctg attgtattgt tcgcgagcaa tgcgctcttg ctcggcttct cccaagggca
     2881 tttcctcggt caccaggtag gatgtgtagg gagtcacaat gccatagcgg atgctcaggc
     2941 gcacaatttg atcgatagtt tccttatccg ggccattcag gcggatttgg ttcaatagat
     3001 aaccgatttt gcgtgtggcc cacagacgtg gaatggaatc gagtacagaa cttcgaattt
     3061 cgtcatcgtc atcggcaaag cgctgctcat cgaagcgaaa agtttgcctg cgaccctcta
     3121 ccttgccacg cagtacgata ttggcaatgc caccctcacg gtaacgcccc accacaatga
     3181 tctgggaccc agcaaacagg tcgggaagcg gatcaggata tatatcataa gtacggatct
     3241 cctcaaagtc cagagatagg tcggtaagca ccggagaact gatctttgcg taaaaggcag
     3301 acaagacctc atccagacgc tcgccaggca gcacgtatga actggagcca tgatgggctt
     3361 gcgcgaggga atccagcaaa aaagtatcca catcgtaccc cacaccgaaa gcaaaaaggc
     3421 gcaggttatc aggtgcagta gtgtccaggt tgtccaggat atcctggcta tcaacgatcc
     3481 cttctgttgg cagtccgtcg gtgaggaaga tgaggtatgt gggtcgctcg cggtcagaca
     3541 tagcagctgc ctccagcaag gcacggttga tgtctgtgct gccttgagca gattgccggt
     3601 ccacccaagc gatggcttca ttggcctcct ccgcaggtcg cagattccgt ccataggtct
     3661 ccagtccggt gctgaagacg ataatattaa atcggtcgtc tggattgaga tggcggagga
     3721 tataacgcaa tgcttcctgc gcctgccgga acttttcacc atccatgctg ccggaacggt
     3781 ccaacaccag aatgacgtct ttggagagcg tctcaattgc ggcttcagga cgtggggcaa
     3841 gcatgatcag gaagaaacca tccgggtcca ctgggtcatc tcgatcgcgg taggtcaaca
     3901 ggtggaaagc ctgctcgtcg cccagggaat aatacaaagt gaagtcagta tctggaagta
     3961 cgtccacatc ttcatagcca gccatcacgt ggcgatcgtc caagcgattg attgcgattt
     4021 catggctggg cgagtacact gcgcgtatgg gtgcctgcga tgagcgcact tccacactga
     4081 tgcttacatt ttctaagggc cagagcgaga atttctccgt actcaaggga tatgtgtagc
     4141 gcaccagacc attctcagcg gtaagcgcct ggacgtactc cagttcaatc cgccgctcac
     4201 cgcctggcgg tataggaaag atgcgcgctt gcaccgcacc acgatcgaca tattccaaaa
     4261 gggctgggtc gctcatgctg cgcacgatct cctcgtacgt cttgcgcgcc tcttcggcat
     4321 ccagtacctt tccctcaaca ggcttgccat caatccaaag ggaaaaggac gaaacggctg
     4381 catctacagg gattgggaag atataggtgc cttcgacctg ccaatcattg gggttatgga
     4441 aaacctggtc tacatgggtt accaccaatt gatcctgtat tgtcaccgag acacgatgat
     4501 aacggatcgc cagctgcaca aaggatggtc tgggggggca cgggaatggt cttgggcagg
     4561 gtggcggtgg gcaaggtccg tccaaacata tcggcgggtc ggggatgata atgccatccg
     4621 ccctggcgga tgtgggcaag gctgccaggg taaagacaaa taataacatc agagcaagct
     4681 tgaacttcat ggtttcctcc cgtaaatcat acggaaacaa ataccagtat aggctaatta
     4741 cgtatgtatc ccatagacgc aggcagctga ggaaaagttc cagtggtata atcctccagc
     4801 accagtccgg tgacaagttt ttaactaatt accggataaa aactcagata ccaaaaccta
     4861 gatcaagcac tgctattctg attaaataat tgaaattcta tgctcgaaaa acttgcaggt
     4921 attgaatcaa gatatcaaga actaaaccag cttctgaatg aagtaggcga cgactaccag
     4981 cgcgctgctg aactgaataa agaacgcatt gacttagaac ctctttacag caaggcccag
     5041 gagtaccgta agtctctgga tcaactggaa caggcccgcc atctcctgga taccgatgac
     5101 gaggaattac gctccctggc agaaaccgaa ttcactgaac tggaagagct catcttccaa
     5161 ttagaagatg aaatcaaagc gatgctattg cccaaagacc ggcgcgacca acgtaacgtg
     5221 attgtggaaa ttcgcgcagg gacaggtggc gaggaagccg ctctatttgc ctcagacctc
     5281 ttccgcatgt acagccgcta cgcagagcgc cggcgttggg atgtggatat cctttcggcg
     5341 aacgagaccg gcattggtgg ctttaaggaa atcatcttta tggtcaaggg acgcagtgcc
     5401 tactctcgct tgaagtatga gtcgggtgtt caccgtgtgc agcgtatccc ggtcaccgag
     5461 gcttcaggac gcatccacac ctcgaccgcc accgtagcgg tactggcaga ggtggacgaa
     5521 gtggagatcg acatccccga tagcgacatt cgcatggatg tgttcaagtc ggctggggcg
     5581 ggcggtcaaa acgtccagaa gaacgccacc gccgtgcgca tcacgcacat tcctacaggc
     5641 attgtggtcg catgccagga tgagcgctcg cagctgcaga accggatgcg tgccatgagc
     5701 atcttgcgcg cacgcctgta cgaaatcgag gaagaaaagc gccgcctgga agttgatgag
     5761 acccgccgtt cccaagtggg tactggcgaa cgcgctgaaa aaatccgcac ctataactac
     5821 ccccagtcgc gggtcaccga ccaccggatt aatatctcaa acttcaactt accaatgttc
     5881 atcgatgggg atatcgacga attcatcgat gaactggcaa cccgtgacga agcagctaga
     5941 ctggccgccg tagggcttga gataga
//
LOCUS       DBNHOH010000985         1918 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1729948, whole genome shotgun sequence.
ACCESSION   DBNHOH010000985 DBNHOH010000000
VERSION     DBNHOH010000985.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1729948"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..>1918)
                     /locus_tag="AC16V7_17815"
     CDS             complement(229..>1918)
                     /locus_tag="AC16V7_17815"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016872936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HIR6259038.1"
                     /translation="SNSWVVSGERTSTGKPLLANDTHLSAQMPSIWYEIGLHCTPQLE
                     TCPFNVTGFSFAGAPGVIIGHNERIAWGFTNLGPDVMDLYIEKLNPANPDQYEVNGEW
                     VDMVKVQETIQIAGGEPVELIVRYTRHGPVVSETYGKLDGFSEAAGVELPASYAISLR
                     WTALEVSHTFPAIWRLNQAGNWEEFRRAMSLFDVPSQNTVYADVDGNIGYQSPGKIPV
                     RASGDGWLPVPGWTDEYEWTGYIPFEEMPYAFNPPQGYIVAANNAVVGPDYPYFITAE
                     WNYGYRAARIMEMIAAHAGPIDVAWMQAMQGDNLDLNAKILTPYLPQAQWGRPILAQT
                     FELLQTWDGQNSMDSAGAALFAVFWKNLLAATFHDDLPEAYYPEGGSRWIQVVRDLVE
                     QPNSPWWDDRTTPDRVETRDEILLRAFTSAVDELEKTQGKDPSKWAWGDLHTLTLRNQ
                     TLGKSGVAPIEAIFNRGPYPTAGGESIVNATGWEANAKHPYEVTWLPSKRMVVDLNDL
                     SNSMTIHTTGESGHAYHPHYADMVDLWRTIQYHPMLWERARVEAEAEGHLRLVP"
BASE COUNT          383 a          588 c          536 g          411 t
ORIGIN      
        1 atagtatcta gacacttttc gcatccatcc ctcccgcgcc aggtaaataa caaaaaaccg
       61 accaacatgc tttggccggt ttcgctattg gctccccatt taccctgctt gtcccccaac
      121 aagcgaccac atgaggctca tggatctccg ccgcctggaa aatactccaa cccaagtata
      181 taaaaactca attcggatgt caatatccta tttgcagtat ttttaaagtt atggcaccag
      241 ccgcaaatgc ccctctgcct cggcttcgac ccgggcgcgc tcccacaaca tagggtgata
      301 ttggatcgtg cgccacaaat ctaccatgtc ggcgtaatgg ggatggtagg cgtgtcccga
      361 ctctcccgtg gtgtggatgg tcatcgaatt gctcaggtcg ttgaggtcta cgaccatgcg
      421 cttggagggc agccaggtga cttcataggg atgcttggcg ttagcctccc agccagtggc
      481 gttgacgatc gattcgcccc cggcagtggg gtacgggccg cggttgaaga tcgcctcgat
      541 gggagccacg cccgattttc ccagtgtctg gttgcgcagg gtcagagtat gcaggtcgcc
      601 ccaagcccac ttagaggggt ctttaccctg cgttttctcc agctcatcca ctgcgctggt
      661 gaaggctcgc agcaggatct cgtcgcgggt ttcaacgcga tccggtgtcg tgcgatcgtc
      721 ccaccaaggg ctgtttggct gctcaacaag gtcgcgcact acttgaatcc agcggcttcc
      781 cccctcgggg tagtacgcct ccggcaggtc atcgtggaat gtagcagcca ataagttctt
      841 ccagaacacc gcgaacagcg ccgcgccggc tgaatccatg ctattctgac catcccaggt
      901 ttggagcagc tcaaacgtct gcgccaggat gggtctgccc cactgtgcct gcggcaggta
      961 gggggtgaga atcttcgcat tcagatccag attgtcaccc tgcatggctt gcatccaggc
     1021 cacatcgata gggcctgcat gcgcggcgat catctccata atgcgcgctg cccggtagcc
     1081 gtaattccat tcggcggtga tgaagtacgg gtaatcaggc ccgacgacag cgttgttggc
     1141 ggccacgatg tacccctgcg gcggattgaa ggcgtagggc atctcctcga aggggatata
     1201 gcccgtccat tcgtactcat ctgtccagcc gggcacaggc agccagccat cgccggaagc
     1261 gcgcacaggg atcttacccg gcgactggta gccgatgttt ccatctacat cggcgtagac
     1321 cgtgttttgc gagggcacat cgaacaggct catcgcccgg cgaaactctt cccaattccc
     1381 cgcctggttc agcctccaaa tagccgggaa ggtgtgcgag acctccagcg ccgtccagcg
     1441 caggctgatg gcgtagctag caggcagctc aaccccggcg gcttcgctaa agccatccag
     1501 cttcccatac gtctcggaga caaccgggcc gtggcgggtg taacgcacaa tcagctccac
     1561 cggctcccct ccagcgatct gaatggtctc ctgcaccttt accatatcaa cccattcccc
     1621 gttgacctcg tactggtcgg ggttagccgg attgagcttc tcgatataca agtccatcac
     1681 atctggcccg aggttggtga agccccaggc gatgcgctcg ttgtgcccga tgatcacccc
     1741 cggcgcgccg gcaaaggaga agccggttac attgaagggg caggtttcca gctggggagt
     1801 gcaatgcaag ccgatctcgt accatatgga tggcatctgc gcgctgaggt gagtgtcgtt
     1861 ggccaataac ggcttgccgg tgctggtgcg ctccccagag accacccaac tgttcgag
//
LOCUS       DBNHOH010000986         4650 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_640424, whole genome shotgun sequence.
ACCESSION   DBNHOH010000986 DBNHOH010000000
VERSION     DBNHOH010000986.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_640424"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="AC16V7_17820"
     CDS             complement(<1..1172)
                     /locus_tag="AC16V7_17820"
                     /inference="COORDINATES: protein motif:HMM:NF025309.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HIR6259039.1"
                     /translation="MSTSLIDADSVLAIDIGSITTRAALFDVVDGRYRFLASGSAQST
                     INAPYNDISEGLRTALDRLQTVTGRILIDADERLIIPSTSEGAGVDIVAATLSAGPPL
                     NVVVIGLLEDISLESAKRVVYTSYAKIVDTLSLNDRRTVEERLDAIIRLRPDLVIVAG
                     GTEGGASQSVLRLVEAVSLAAHMLPQGHRPQILFAGNQSLRDEIKATFDKLAPLQFAT
                     NVRPTLENEQLESAQLHLAHIARQVRSQQIIGVTELDNWSGGGLLPTSTAFSRVIRFL
                     SKVYDSTKGVLGVDVGSSATSIAASFEGNLVSRVYPQLGLGHGLKEFLNVSPLQGITR
                     WLHIGLSDDDVRHYIHNKALYPASLPVTPEELAIEQALARQAMQISIKKTSNGFPE"
     gene            complement(1182..1880)
                     /locus_tag="AC16V7_17825"
     CDS             complement(1182..1880)
                     /locus_tag="AC16V7_17825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6259040.1"
                     /translation="MSLADLSLTDMLGSIIGFFLTLMVFSYAFGDNAMFRTAIHIFIG
                     VASGYAAVVAWYNVIWPQLIIPMVFGTQADRIDILFPSVLGVLLLFKASPRLSPLGNP
                     AVAYLVGVGAATAIGGAILGTIFPQSLASINLFDQQNLQQGGEYVIQLAKGSVVLIGT
                     LATLAYFHFGARQYPNQPAGRPKWLEGLAWIGQIFIAIALGSLFAGIFAATLTALIER
                     VNFIIDFIFALIPS"
     gene            complement(1877..4330)
                     /locus_tag="AC16V7_17830"
     CDS             complement(1877..4330)
                     /locus_tag="AC16V7_17830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ICP22 family protein"
                     /protein_id="HIR6259041.1"
                     /translation="MDEVRCPMCGKLNPEELDTCQYCQARLKPLNISSAPEEDAEITS
                     SSASFDETPSQKKPPDQTPALPEWLQPDWLQSLRQGSGTDELGEIEPEDQLPDWSSEE
                     VDPGDQETESAESSAPEDSSEKPDWLEGLRAANQAEPETPSEQDSESTYSEEMPEDEI
                     PDWLQRIRARQSSDEPELPLEEPDAESAEFLAKLRMSPDADETDSSEQADAEEAQPGP
                     DEEDSQVPDWLAEYAESGTESTPPELPDWLKSDELPAEPEDEEDSLAEFPSRMTPSQP
                     PFTEPFLEEDIELESATELPAETPPDKPPFTEPVDEEEAEGEIADWLADLEIPAEQEE
                     MTSLPVEDEEKTSFAFEAEPVEDQPDWLQDIEIKDGISPKEDEQDQDSELEVVAPFTL
                     DEESADLIQEELPDWIKEIPATEAQTEAEPSEEAAGELEPAELPTWLESMRPIESVAP
                     SAPASDELDQIVESSGPLAGLQGILPAEPEIAREMKSPVYTVKLQVSENQQSLASRLA
                     EMVKSEGIAAPIPRAPTISSQAVVRITLFLILLVSVVWPLYTGDLGAPAPELSPEVLD
                     AQLLINALPDGGNVLLAIDYQPAYSGEMNATTASLLDHLMIKGAYLTLVSTSTTGPAQ
                     AEQLVRYVNNQMGHQYRDTSQYANLGYIGGGLSGLRSFAQSPQQIMPYALNASTADES
                     TVWINGRLSNVQALSDFALTVVISDSPETARNWIEQVGPALEDTPLLLVVSAQAEPLV
                     RPYYEGYPKQAKGLVVGLSGGVAYESSMPRPGIARRYWDAYSYGIPTAVLLILIGGLV
                     NIVLAYLPTLKPGQGRAKR"
     gene            complement(4333..>4650)
                     /locus_tag="AC16V7_17835"
     CDS             complement(4333..>4650)
                     /locus_tag="AC16V7_17835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6259042.1"
                     /translation="IQVPVESSLLAILVVVLTYAAMRLIRRRLNLFSIIFIGTGLITL
                     LGIAPLFGVVIPGLHGAFGFRTLIAQIPAVAGARGILLGVALGTVATGLRILMGADRP
                     YRG"
BASE COUNT         1067 a         1179 c         1264 g         1140 t
ORIGIN      
        1 ttttcaggaa aaccattgct tgtctttttt atactgatct gcatggcttg ccgcgccagc
       61 gcttgctcta tggcaagctc ttctggggta acgggcaagg aggcagggta gagtgctttg
      121 ttgtggatat aatggcgcac atcgtcatct gacaacccga tgtggagcca gcgggtaata
      181 ccctgcaacg ggctgacgtt taggaattcc ttcaagccgt gtcctagtcc aagctggggg
      241 tatacgcgac tgaccagatt gccttcaaag gaagcggcta tgctggtagc agaagaacct
      301 acgtctacac ccagcacacc ttttgtggaa tcataaactt tactcaagaa gcgaatcaca
      361 cggctaaagg ctgtggatgt aggcaataat ccaccaccag accagttgtc cagctctgta
      421 acaccgatta tttgctggct acgcacttgc cgagcaatat gggcaagatg caactgggcg
      481 gattccaact gctcattctc caaggtggga cgaacgttgg tggcgaattg cagtggcgcc
      541 agtttatcaa atgtagcctt tatctcgtct cttaacgact gattgccggc aaacagtatc
      601 tgtgggcggt ggccttgagg tagcatatgt gcggccagac taacggcttc tacaagcctg
      661 agaacagact gggatgcgcc accttcagtc ccacccgcga cgataaccag atcggggcgc
      721 aggcggatga tcgcatccag gcgttcttcg acagtgcggc ggtcattcaa gctgagggtg
      781 tccacaattt ttgcatagga tgtgtaaacc actcgcttgg cactctccaa ggatatgtct
      841 tcaagcaaac cgatcacgac tacattgagc gggggtcccg cagatagtgt cgctgcgacg
      901 atgtctacgc ctgcaccttc agatgtgcta gggatgatga ggcgttcatc ggcgtcgatt
      961 aggatacgcc cggtgacagt ttgtagtcga tctaaggcgg tgcgcaggcc ttcgctaata
     1021 tcgttatagg gagcgttgat cgtggattgg gcggatccag aggccaggaa gcggtaacgc
     1081 ccatccacaa cgtcaaataa tgcagcccgt gttgtgatgc tgcctatatc aatggcaagt
     1141 accgaatctg cgtcaattag cgaggtgctc atggaaatct attaggatgg aataagcgcg
     1201 aagatgaaat caataataaa attgacccgc tcgatcagtg cggtcagtgt ggcggcaaag
     1261 atcccggcaa acagcgatcc caaggcgatg gcaatgaaaa tttggccgat ccatgcaaga
     1321 ccttccagcc acttaggcct acccgcaggt tgatttggat attggcgggc accgaagtga
     1381 aagtatgcca gagttgccag agtgccgatc agaacgacac tgccctttgc cagttgtatt
     1441 acgtactctc ctccttgttg aagattttgt tgatcgaaca agtttattga agcgagggat
     1501 tgagggaaaa tcgtccccag gattgccccg ccaattgctg tggctgcgcc taccccaaca
     1561 aggtaagcca ccgctggatt ccctagcggc gataagcgag gcgaggcttt gaaaagcaat
     1621 aagactccca gaacagatgg gaaaaggata tcgattctat ccgcttgtgt cccaaatacc
     1681 attgggatga tcagttgagg ccagatcacg ttataccagg ctaccacggc agcatatcca
     1741 gaggcgacac cgataaagat atggatggca gtccgaaaca tggcgttgtc gccgaaggcg
     1801 tagctgaaaa ccatcagcgt caagaagaag ccaatgatag agcccagcat atcggttagg
     1861 ctgaggtcag ccaggctcat cgtttggccc tcccttgacc cggtttcagg gttggcagat
     1921 acgcaagcac tatgttaact aatcccccga tcaggatcaa taatacagca gttgggattc
     1981 catagctata ggcatcccag taacggcgag caatgcccgg gcgtggcatg ctactttcat
     2041 aagccacacc accgctcaac ccgaccacca atcctttggc ttgctttgga tagccttcgt
     2101 agtatggacg caccagtggc tcagcttggg cgctgaccac caatagcaac ggagtatcct
     2161 ccaatgctgg tcccacctgc tcaatccaat tcctagccgt ctctgggcta tccgaaatca
     2221 ccactgtcag ggcaaaatcg gacaatgctt ggacatttga aagccgccca ttaatccaga
     2281 cggtactttc gtctgcggtg ctggcattga gagcgtaagg catgatctgc tggggagatt
     2341 gcgcaaagct gcgcaatcct gagagcccgc cgccgatata tcccaggttg gcgtattgac
     2401 tggtatcgcg atattggtgt cccatctggt tatttacata gcggactaac tgctctgcct
     2461 gggctggtcc ggtggttgag gtggatacca gggtcaggta tgcgcctttg atcatcaggt
     2521 gatccagtaa agatgcagtc gtggcattca tctctccaga atatgcgggc tggtaatcga
     2581 tagccagtaa aacgttgccg ccatctggaa gggcgttaat aagcaattgt gcatccagga
     2641 cctcaggcga taattctggg gcgggtgcac caagatcgcc ggtatataag ggccaaacga
     2701 ccgataccag gagtatcagg aagagcgtga ttcttactac agcttgggag gagatggttg
     2761 gggcgcgagg tatgggggct gcgatgcctt ccgatttcac catctccgct aaacgggagg
     2821 cgagagattg ctgattttcg gaaacctgca acttgaccgt gtacacggga gatttcatct
     2881 cacgtgcaat ctctggttca gcgggcaaaa tgccttgtag acccgcaagt ggaccgctac
     2941 tctctactat ttggtcgagt tcatcgctag ctggtgcgct tggcgctaca gactcgatgg
     3001 gtcgcatgga ttcaagccag gtgggcagct ccgcaggctc aagttcgcca gccgcttctt
     3061 ctgagggttc agcctctgtt tgcgcttcgg tcgcagggat ttccttgatc caatcgggca
     3121 actcttcctg gattagatcg gcgctctctt catccaatgt gaagggggcc accacctcaa
     3181 gctctgaatc ctgatcttgc tcatcctctt tgggactgat tccatctttg atttcgatat
     3241 cctgcagcca atcaggttgg tcttcgactg gttctgcctc gaacgcgaag gaagttttct
     3301 cttcgtcttc gactggaagt gaagtcatct cttcttgctc ggcgggtatt tcgagatcgg
     3361 ctaaccagtc agctatttct ccttcggcct cttcctcatc gacaggctca gtaaagggag
     3421 gtttatcagg gggtgtttca gcaggcaatt ctgtagcgga ttcaagctcg atatcttctt
     3481 ccaaaaatgg ctctgtaaaa ggtggttggc tgggcgtcat tcgggatgga aattccgcta
     3541 aagaatcttc ttcatcttcg ggttccgcgg gtaattcatc ggatttcaac caatcaggaa
     3601 gctcaggggg cgtactttcg gtgcctgatt cagcatattc tgctagccag tcgggaacct
     3661 ggctgtcttc ttcatctggg ccgggctggg cttcttcggc gtccgcttgc tcagacgaat
     3721 cggtctcatc ggcatcgggg ctcattcgca gtttcgccag gaattccgcg gattcagcat
     3781 ccggctcctc taaggggagt tcaggttcgt ctgagctctg gcgagcacgg atgcgttgga
     3841 gccaatcggg aatctcgtct tcaggcattt cttctgagta agttgattct gagtcctgct
     3901 ctgagggtgt ctcgggctcg gcttggtttg ccgcccgcaa gccttctaac cagtccggct
     3961 tctctgagct atcttcaggg gctgaagatt cagcggattc ggtttcttgg tctcctgggt
     4021 cgacttcttc gctagaccaa tcggggagct gatcttcggg ctctatctcc ccaagctcat
     4081 cggtaccgct cccctgtcgc aatgattgaa gccaatctgg ttggagccac tcgggtagag
     4141 caggagtttg atccggtggc tttttttgag atggcgtctc atcaaaggaa gccgaggaag
     4201 atgttatttc agcatcttct tctggagcag aggaaatgtt aagtggcttc aggcgcgctt
     4261 ggcaatactg gcaggtgtcc aattcttccg gatttagttt gccacacatt gggcagcgca
     4321 cttcgtccat aattaacccc gatatgggcg gtcagcgccc atcaggatac gcaaacctgt
     4381 ggcaacggtc cctaatgcca cgccaagcag tatgccacga gcaccagcta ctgcgggaat
     4441 ttgggcgatc aaggtgcgga atccaaacgc gccatgcaag ccaggaataa ctacaccaaa
     4501 taatggtgcg atacccagga gcgtaatcaa acctgtgcct ataaatatga tcgagaacag
     4561 gttgagtctt cttcttatca ggcgcatagc ggcgtaagta agcacgacga ctaatatggc
     4621 cagaaggctg ctttctactg gcacctggat
//
LOCUS       DBNHOH010000987         2668 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1217131, whole genome shotgun sequence.
ACCESSION   DBNHOH010000987 DBNHOH010000000
VERSION     DBNHOH010000987.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1217131"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..1053
                     /locus_tag="AC16V7_17840"
     CDS             187..1053
                     /locus_tag="AC16V7_17840"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6259043.1"
                     /translation="MQQFSLLLTSLPDGILLGFVYGVAAMGLTLIWGVMNVINLSHGP
                     IIALGMFGTFFIFIRLGINPYIGLLLIAFLGLIIGVLIYFGAVHRVINAPHLSSLLAT
                     FSVNMIIIGLGTAALTTSPYNVDFNMGSFRIGLTTILGTRLIAALIAILFTGGLYLFL
                     YKTRPGKFIRAVANNRAAAELMGIPSTRILALSFGLGTMLAAVSGALIATFFPFSILS
                     GSGYELKSFVIGVLGGLGNPIGALLGGLILGVLEGIIPAFMETSWVPVIEFGLFVVIL
                     LVRPHGLFGIKK"
     gene            1050..2021
                     /locus_tag="AC16V7_17845"
     CDS             1050..2021
                     /locus_tag="AC16V7_17845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012941224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR6259044.1"
                     /translation="MNKWRNHLGLLIPLLAVAAMVLWPIVTGNVSNREIMFTILKAIA
                     LASSLNILLGYTGYVSFGHIVFYGFGGYVGLYLVNEQGWSIWLGMLVGGLASGALAYL
                     LGKAILRLRGAYFALATIGINEAMKAFINNFEPFGGPIGMTLNFSVYKAYGGAAQALS
                     MSFYIMAFLTFATLIISYLVRTSKFGLGLRAIRENEDAAEVMGVVAPEAKTWAYVLSA
                     IIPGMVGVLFFFKNGNVEPYVAFPLHASIELLVMVMLGGQGTVLGPVLGALAYQGMRG
                     YLVTSTFFKDIQLSVSGVLLLLIVLFIPAGAIGWLRHRFSVLHKVLP"
     gene            2018..>2668
                     /locus_tag="AC16V7_17850"
     CDS             2018..>2668
                     /locus_tag="AC16V7_17850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012941225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6259045.1"
                     /translation="MILQVEGVSKRFGGLQALIRVTFDLPDGQILGLIGPNGAGKTTL
                     FNVINGVYPPNEGRVTFRGTDVTGMKPYQLAHLGLARSHQIVLPLNELSVRENVMVGA
                     CFGREKQSLGGAGKTAEEILDFVGLADRAEQLAGSLNIAQKKRLEMARALASRPHLLL
                     LDEVLAGLNPAEITTMVETIRRIRQQGVTIIMIEHVMHAVMNVSDRVIVLDYGEQIA"
BASE COUNT          593 a          650 c          672 g          753 t
ORIGIN      
        1 cctgagggga tggtttctat ttctccacag aaatgctaca ttatgcacac taccagaatt
       61 tagaaacaga taatatggat caattcagat tcttctattc ctctaaacaa aacctgcgtg
      121 caaccggtgg gaatacgcgt ccagttttat tacgggacaa acttaacatc tatgatggga
      181 gtaattatgc aacaatttag cttgctactg acatccctcc cggatggcat tttgcttggg
      241 tttgtgtacg gtgtggcggc catgggtctg acgctgatct ggggagtgat gaatgtcatc
      301 aacttgtctc atggcccgat catcgcttta ggaatgtttg gaactttctt tatttttatc
      361 cgactgggta tcaatcccta cattggcctg ctcttgatcg cattcctcgg attgataatt
      421 ggggtgttga tttatttcgg agcagtccac cgcgtaatca atgcccccca cctatcatcg
      481 ctgctagcga cgttttctgt caacatgatc atcatcggtc taggaacagc cgccctaaca
      541 acctcgccat ataatgttga tttcaatatg ggcagtttcc ggattggctt aaccacaatt
      601 cttggaacgc gcctgatcgc ggcattgatc gcaattctgt tcactggtgg gctttatcta
      661 tttttgtata aaacccgacc cgggaagttc atccgtgctg ttgccaacaa tcgcgccgca
      721 gcagaattga tgggtatacc ctctactcgc atcctggcac ttagctttgg gctaggcacc
      781 atgctggctg cagtttctgg ggcgttgatc gccacatttt tccccttcag catcctatcg
      841 gggagcggtt acgaattaaa gagcttcgtc atcggtgttc taggcggcct gggcaatccg
      901 atcggtgcgt tattgggagg cttaatcctg ggtgtgcttg agggtattat tcccgctttc
      961 atggagactt cttgggtacc ggtcattgaa tttgggctgt ttgtggtcat ccttttagtt
     1021 cgaccgcacg gactctttgg aataaagaaa tgaataaatg gcgaaatcac cttggtcttc
     1081 ttataccact attggcagtt gcagcaatgg tcctgtggcc gatcgttaca ggaaatgttt
     1141 ccaaccgcga aataatgttt accatcttga aggctatcgc attggcctcc agcctgaata
     1201 ttttactcgg ttatactggc tatgttagtt ttggccatat cgtattctat gggtttggcg
     1261 gctatgttgg tttgtactta gtcaacgaac aaggctggtc gatatggctg gggatgctgg
     1321 ttggcggatt agcctctggg gctttggctt atttgctggg taaagcgatc ttgcgtttac
     1381 gcggcgctta ctttgctttg gcaacgatcg gcattaatga agccatgaaa gcattcatca
     1441 ataattttga gcccttcgga ggccctatcg gcatgacact caacttctca gtctacaaag
     1501 cctatggagg cgcagcccag gcattgagca tgtcatttta tattatggcc ttcctgacat
     1561 ttgccaccct catcattagc tacctggtcc gcacctcgaa gtttggctta ggtctgcgag
     1621 caattcgtga gaacgaagac gctgccgaag tcatgggtgt tgtagctccc gaagccaaga
     1681 cctgggctta tgtgctttca gccatcatac ccggaatggt gggtgtgtta ttcttcttca
     1741 aaaatggtaa cgtggagcca tatgtcgctt ttcccctgca cgcctccatc gagttattgg
     1801 ttatggtgat gttgggaggt cagggtaccg ttctcggacc agtccttggg gcactggcct
     1861 accaaggcat gcgcggctat ctcgtaacca gtactttctt caaagatatt cagctttccg
     1921 tttccggggt gcttttgtta ttgatcgtgc tctttattcc ggcgggtgct atcggctggc
     1981 tgcgccatcg cttctcagtt ctccacaagg tgctaccatg atcttacaag tagaaggagt
     2041 atccaaacgc ttcggcgggc ttcaagcact catcagggtt acattcgacc taccagatgg
     2101 gcagatttta gggctgatcg gacccaatgg cgcaggaaaa actaccctgt ttaatgtcat
     2161 caatggagta tatccaccca atgaaggcag ggtaactttt cggggcactg atgtcaccgg
     2221 gatgaagcct tatcagctag cccacctggg cttagcccgc tctcaccaga ttgtgcttcc
     2281 actcaacgag ctcagtgtgc gcgagaacgt catggtgggc gcctgctttg ggagagaaaa
     2341 acaaagcctt ggcggagcag gcaaaacagc agaagaaatc ctggatttcg tcggcctggc
     2401 ggatcgtgca gaacaacttg ccggcagcct gaacatcgct cagaagaaac gcctcgagat
     2461 ggcacgcgcc ctggcatccc ggccccacct actgctcttg gatgaagtgc tggctggatt
     2521 aaaccctgct gaaatcacaa caatggtcga gactatccgg cgcatacgcc agcaaggtgt
     2581 cacgatcatc atgatcgaac atgtcatgca cgctgtaatg aacgtttctg accgggtcat
     2641 cgtgctggat tacggtgagc agatcgcc
//
LOCUS       DBNHOH010000988         1942 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1281364, whole genome shotgun sequence.
ACCESSION   DBNHOH010000988 DBNHOH010000000
VERSION     DBNHOH010000988.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1281364"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1942
                     /locus_tag="AC16V7_17855"
     CDS             <1..>1942
                     /locus_tag="AC16V7_17855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6259046.1"
                     /translation="LPAAAHTPQNQPDQQIKISADQLTDVSPALLDASDQAQIPFAAI
                     DSRNLPVDLREIPERFDMRTANSATFQDPGGIFQTVISPDTMHYLDELGNWQVIEPVF
                     QQLDDSFVVQRNSLKSRAGARRAWMSGMVGETAAIWEASELGVAGVDGNFTSLAKALA
                     ETPYPAERQLAGRLLHYVGGWSDPTLVEEIASAPDALEHSLILGERPQIDVKDGYLEM
                     RATLDLLPGASLWVDGEKVDGEARTGGMIEIRDAAGKPALALSPVVAFEQANPMITVD
                     GEYLISPGDELGSWQVRVRTPLAWWLEPARQYPLVIDPEMRVLKSTGYADGMGWVRSN
                     DTKAFTLGGIRLGAHLPYWDTKSRGYVQFNSLPSLLTNSSLQVLSASLDVTPTLPPEA
                     MPLYKNSPKDWEHEKISRNTELHYLGACPDTSGCNGLSFLTGAPSNNWDNSPEGTLVG
                     TKLLKAGPASGGGTPQTTTWDVTTQIQSWYTNHFTDNTQRPGPTFMMRFPDTTKENCP
                     APGPYYFGFVPGKVNEINTSWQYVPYCIWFDIPAGSVQLRIAYQSLALKINDNLLNTP
                     GVPSFLEGTFENTNHQYMLTPAVGLQKHWRAVAVRGNHDFDEALPTRVGLQVSDYTTD
                     PSNPELLASGVTKGPDETAFVF"
BASE COUNT          432 a          532 c          561 g          417 t
ORIGIN      
        1 ccctgcctgc agctgcccac acacctcaaa atcagccgga tcagcagatc aaaatatctg
       61 ctgatcagct cacagatgtg tccccggccc ttctggatgc ctctgatcag gcgcaaatac
      121 ctttcgccgc catcgactcg cgtaatctac ctgtggatct gagggagatt ccagagcggt
      181 ttgacatgcg tacggccaat agcgctactt tccaagatcc aggggggatt ttccaaacgg
      241 taatctcccc cgatactatg cattacctgg atgaactcgg caattggcag gtgattgaac
      301 cggtcttcca acagttagac gatagtttcg tggtgcagcg caattccctg aagagccgtg
      361 caggggcgcg gcgagcctgg atgtcgggga tggtggggga gacggcagcc atctgggagg
      421 catccgagtt aggggttgct ggcgttgatg ggaactttac ctcgttagcg aaagcgctgg
      481 cggaaacccc ttaccccgca gaacggcaac tggctggcag gctactgcat tatgtgggcg
      541 gctggagcga cccaactctg gtagaggaga ttgccagcgc gcccgatgcc ctggaacact
      601 cgttgatcct gggggagcga ccgcagattg atgtcaagga tggatacctg gagatgcgcg
      661 ctactttgga tctgctgccc ggcgccagcc tgtgggttga cggggaaaag gtggatgggg
      721 aggcgcgtac cggcgggatg atagagatca gagacgcggc agggaagcca gccctggctc
      781 tctcccccgt agtggctttt gagcaggcta atcctatgat cactgtggat ggtgaatacc
      841 tgatctcccc tggggacgaa ttgggtagct ggcaggtgcg cgttcgcacg ccattggcgt
      901 ggtggttgga acctgcccgc cagtatccgc tggtgattga tcccgaaatg cgggtgctga
      961 agagcacagg ctatgcagac ggtatgggtt gggtgcgcag caatgacacc aaagccttca
     1021 ctctgggcgg tatccgcctg ggggcgcacc tgccctattg ggatacgaaa agccgggggt
     1081 acgtgcagtt caacagcctg ccatccttgc tgactaattc gagcctccag gtgctgtcgg
     1141 cttcgctaga tgtgacgccc accctgcccc ccgaggcgat gccgttatat aagaattctc
     1201 ctaaagattg ggagcatgag aaaatctccc gcaacaccga gcttcactat ttgggcgctt
     1261 gcccggatac cagtggctgt aatggactgt ccttccttac cggcgctccc tccaataact
     1321 gggacaacag cccggaaggg acactggtcg gcaccaagct cctgaaagct gggccggcgt
     1381 ctggtggggg tacaccccaa acaaccacct gggatgtcac cacacagata caaagctggt
     1441 atacgaacca ttttaccgac aatacacaac gccctgggcc cactttcatg atgaggtttc
     1501 cagacacgac gaaggagaac tgcccagcgc caggccctta ttattttggc tttgtccctg
     1561 gtaaggtcaa tgaaattaac acatcctggc agtatgtacc ttattgtatc tggttcgata
     1621 tcccggctgg aagcgtgcag cttcgtatag cctatcagtc cttggccttg aaaataaacg
     1681 ataacttgct gaatactcct ggtgtgccga gctttttaga gggcacattt gaaaatacca
     1741 accaccaata catgctgact ccagccgttg ggctacaaaa gcactggcgt gcggttgctg
     1801 tgcgcggcaa ccatgacttt gatgaggccc taccgacccg cgtagggctc caggtaagtg
     1861 actacacaac cgacccgagc aaccccgaat tgctcgccag tggggtcacc aaaggaccag
     1921 atgagaccgc gtttgtgttc tt
//
LOCUS       DBNHOH010000989         1729 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1152930, whole genome shotgun sequence.
ACCESSION   DBNHOH010000989 DBNHOH010000000
VERSION     DBNHOH010000989.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1152930"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..1689
                     /locus_tag="AC16V7_17860"
                     /pseudo
     CDS             178..1689
                     /locus_tag="AC16V7_17860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785077.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
BASE COUNT          407 a          431 c          531 g          360 t
ORIGIN      
        1 atcatcagat catgacgcct ggcagccaag cataggttac atgtcactcg catttgcccg
       61 taattcggag tatcatagaa gatgatagac gaaaaacaga tcttagatgt tgggcaggtc
      121 ctgaaagcct gatttccact agttactgcc cacgaacatc tgaaatttgg aggaaccatg
      181 caagacctga ccgaaaaaat cctcgaattg gtgcgcctga cttcttccga tttgcccccc
      241 gatgtagaac acgcgttgcg ccaggcggta gagaaagagg agccgggctc ggcagcacgc
      301 ggcgctttgg agacgatcat caaaaacgtg gagctctctc gggctaattc caccccaata
      361 tgccaggaca ccgggacgcc gatcttctac gtgcgccatc ctgaaggctg gagcacgcgt
      421 aagctgcgtg cgcagattga gagcgccgtc gagcaggcga cacaacgggc ctacttgcgc
      481 cctaactcgg ttgactccct gactgggaag aacactggta acaacctggg cgattctcac
      541 tttccaacaa ttcatttcga agaggtcgag ggggatactc tcaccatcga actgatgttg
      601 aaaggtgggg ggtgtgagaa tgtcggggcg cagtattcgc tacccaacag cgctttagga
      661 gcaggtcgtg atctggctgg tgtgcgcaag gtggttttgg acgctgtgca caaagcccag
      721 ggacgaggct gcgctccggg catcctgggt gtagctattg gcggagatcg cggctcttcg
      781 tattacggct ccaaagaggt gctgttcaac cagatagatg acgctaatcc gaacccagag
      841 ctggctgccc tggaggaacg cctgaccggt gaagccaacc agttaggcat aggcccgatg
      901 ggtttcggcg gcaacacgac cgtgctgggg acgaagatcg ccagcctgaa ccgcttgccc
      961 gcaagctact ttgtctctgt gtcctacatg tgctgggcat accgcaggcg aaggatgagc
     1021 ttaaaggacg gggtggtgag ttattcgtga taaaggaagg aagatatgcg ccagattaat
     1081 ctacccatcg acgacgaaac catccgcagc ctcaaggtag gcgaacccgt gttgctcacc
     1141 ggtgtgatgg tgaccggccg agataacgtg cacaagtgga tgatcgagac atttatcaag
     1201 aagacccgcc agccaacgga agatgacctg atggtatatg aggcgatcaa gcccttactc
     1261 aagggtggcg tgatctacca ctgcggcccg gtggttgccg ggttggagac taaggattac
     1321 cgctttgtgg ctgccggtcc gaccaccagc agccgcgagg agccctacca aggcgatgtg
     1381 atgcgccact tcaatattaa aggtgtggtt ggtaaaggcg ggatgggtgc caagacactg
     1441 gctgcttgcc aggaggtccc gggggtttat ttccatgctg tgggtggtgc ggcttccctg
     1501 atcgcccaga cagtgaagaa agtcattggg gtatacaagc tggaattcgg cgttcccgag
     1561 gccatgtggg ttatccaggt ggtggatttc cccgctgtgg tgaccatgga cgcgcatggc
     1621 agcagccagc atgctgtgat tgaggagcag tctagagcgg ctctcgagga actgctggca
     1681 ctttcttaat taaataggac ttacgcagtt gaagaggaaa ggagggggg
//
LOCUS       DBNHOH010000990         2830 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_704970, whole genome shotgun sequence.
ACCESSION   DBNHOH010000990 DBNHOH010000000
VERSION     DBNHOH010000990.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_704970"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="AC16V7_17865"
     CDS             complement(<1..201)
                     /locus_tag="AC16V7_17865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6259047.1"
                     /translation="MDILHLIDRLEELLNQSRPFPFTHNVIIDEDRMLDIIDQMRVAI
                     PEEIKKAQQVMNQRDRILAQAQE"
     gene            complement(290..814)
                     /gene="coaD"
                     /locus_tag="AC16V7_17870"
     CDS             complement(290..814)
                     /gene="coaD"
                     /locus_tag="AC16V7_17870"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIR6259048.1"
                     /translation="MVRAVFPGTFDPIHLGHIDIADRATQLFDELVVAVYDRPLKSLL
                     FSPEERIALVKQSFKDNPKITVMGYSGLTVEYCQAIGAHVIVRGLRVFSDFEYEFRMA
                     LANHRLAPNIELVALITAEEHTFLSSSTVREIASLGGDVSSMVPEHVKLALQARFTEL
                     GDGQQLVPTTSLRD"
     gene            complement(814..>2830)
                     /gene="recG"
                     /locus_tag="AC16V7_17875"
     CDS             complement(814..>2830)
                     /gene="recG"
                     /locus_tag="AC16V7_17875"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR6259049.1"
                     /translation="MHTLRDMMYYFPRKYLDYTQLKPINRLWYGEEVTVIGTVQNITS
                     RPRRGRPSQIAEAVISDGTGALRVTWFNPWLAKRLQTGAHISLSGKIDQYLGRLVMVN
                     PEWETLEQQQLSTNRIVPMYSLTAQITQRWLRRLMDQVITYWSPRIPDHIPEKIRQNA
                     DLLDLPTALQQIHFPENWDLLKAARHRLAFDEIFLLQLGVLRQKHMWQERTARAFEVE
                     DNWLATQIAGLPFPLTEAQQRAVNDLRTDLASGIPMNRLLQGDVGSGKTVVAALAISI
                     VTHLGAQAALMSPTSILAGQHYKSMMSLLAGVNGQATQPEGGYPIRPEAIRLMIGATP
                     EAEKREIREGLAEGSIKLVIGTHALLEDPVTFSNLQLAIVDEQHRFGVEQRATLRSKG
                     ENPHLLVMTATPIPRSLALTVYGDLELSVIDEMPPGRQSVHTYVLTPQERERAYHLIS
                     SQIEAGRQAFVIYPLVEESEQSESKAAVDEHARLQNEIFPNLKLGLLHGRLHPDEKED
                     VMSRFHSGEFQILVSTSVVEVGVDVPNATVMLIEGANRFGLAQLHQFRGRVGRGGDQA
                     YCLLIPDTPDAAENERLQAMTETDDGFVLAERDLEQRGPGEFLGTRQSGYSELQLANL
                     TDIYLIEKARRQAQVVFEDDPGLQQPAHQLLAAAMESFWSGGKGDIS"
BASE COUNT          635 a          729 c          772 g          694 t
ORIGIN      
        1 ttcttgcgcc tgcgccaaga tgcggtcgcg ctggttcatt acctgctggg cttttttgat
       61 ctcttcggga atagctacgc gcatctgatc gatgatatcc agcatgcgat cttcatcgat
      121 gatgacgtta tgtgtaaatg gaaacggccg gctttgattg agcagttctt caaggcggtc
      181 aatcagatgc aaaatatcca caggctgcct ccgcgtgatg aaataattat caatagatta
      241 cacgcaaata ccgatttcgt caactatccg tgcaaaatgt atgccaatct caatcccgca
      301 gggaagtggt tggaacaagc tgttgtccat cgcctagctc agtaaagcgt gcttgcaagg
      361 ccaacttgac atgctcaggc accatgctgc tcacatctcc acccagagag gcgatttcgc
      421 gcacagtaga tgaagagagg aacgtgtgtt cttcggcggt gatcagagcg accagctcaa
      481 tatttggagc caggcggtgg tttgcaagtg ccatacggaa ctcgtattcg aaatccgaga
      541 aaactcgcag gccacgcaca atcacatgag cgccaatggc ttggcagtat tccaccgtaa
      601 gcccgctgta tcccatgaca gtaattttag ggttgtcctt gaaggattgc tttaccagcg
      661 caatccgctc ctcaggcgag aaaagcaatg atttcaaagg gcggtcataa accgccacga
      721 ccaattcatc gaagagttgt gtggcgcggt ctgcgatatc aatgtgtccc aagtggattg
      781 ggtcgaaggt acctggaaat actgctcgaa ccattagctg atatcccctt ttccgccgct
      841 ccaaaagctt tccatggctg cagctagtag ttgatgggcc ggctgttgca ggccgggatc
      901 gtcttcgaag acgacctgcg cttgccggcg ggctttttca atgagataga tgtcggttag
      961 attggcgagt tgtaattcgg agtaaccaga ctggcgcgtt cccaggaatt ctcccgggcc
     1021 gcgttgctcc agatcgcgct ctgcaaggac gaatccatcg tcggtctcgg tcatagcctg
     1081 caggcgctca ttctcagcgg catctggcgt atctgggatg agcaggcagt aagcctgatc
     1141 gcccccgcgt cccacccgcc cgcggaattg gtgcaactgg gctaatccga agcggtttgc
     1201 cccttcgatc agcatcactg tggcattggg cacgtccact cctacttcca caacggaagt
     1261 tgagaccagg atttgaaatt caccgctatg gaagcgagac atcacatctt ctttttcatc
     1321 ggggtgtaag cgtccatgta gaagaccaag cttaagattt ggaaagatct cattttgcag
     1381 gcgggcgtgt tcgtccacgg cggccttgct ctcgctctgc tcgctctcct caaccaaggg
     1441 atagatcaca aatgcctggc gaccagcctc gatctggctg gagatcaaat gataggcgcg
     1501 ctctcgttct tgaggggtta gaacataggt gtgcacagac tgccggccgg ggggcatctc
     1561 atcaattaca gatagctcta gatcaccgta gaccgtcagg gctagcgagc gagggatagg
     1621 ggtcgcggtc atcaccagta gatgggggtt ctcccccttg gagcgcaggg tggcgcgttg
     1681 ctctaccccg aaacggtgtt gctcgtcaac gatggctaat tgcaggttgg aaaatgttac
     1741 cgggtcttca agcagggcgt gggtacctat caccaacttt atggagccct cggctagacc
     1801 ttcgcgtatc tcccgcttct cggcttctgg tgtagcacca atcattaatc ggatggcttc
     1861 tggacggata ggatatccgc cttcaggttg ggtagcttgc ccatttaccc ccgctagcaa
     1921 gctcatcatg ctcttatagt gttgaccagc caggatgctg gtgggtgaca ttaaagctgc
     1981 ttgggcaccg agatgagtca caatcgaaat ggctaatgca gccacaacag ttttacctga
     2041 acccacatca ccttggagaa ggcggttcat gggtatgcct gatgccaggt cagtgcgcaa
     2101 atcattgacc gcgcgctgtt gagcttccgt gagagggaat ggcaacccgg ctatctgggt
     2161 tgccagccaa ttgtcctcaa cttcaaatgc gcgcgccgtg cgttcttgcc acatatgttt
     2221 ttgacgcagc acgcccagtt gtaacaggaa tatctcgtca aatgccagac gatggcgggc
     2281 agccttcagc aaatcccagt tttctgggaa gtgaatctgc tgcaatgcgg tcggtagatc
     2341 gagcaggtcg gcgttttgac gtatcttttc tgggatatgg tctgggatgc ggggcgacca
     2401 ataggtaatt acttggtcca tcaagcgacg cagccagcgc tgggtgatct gagcggtgag
     2461 cgaatacatt ggcacaatgc gatttgtgct caattgttgt tgttccaggg tctcccattc
     2521 gggatttacc atcaccaaac gtcccaggta ctggtcaatt ttgcctgata gggaaatatg
     2581 cgccccagtt tgcaggcgtt tagccagcca aggattgaac caggtaacgc gcagcgcgcc
     2641 tgtcccatcg ctgatgactg cctcagcgat ctgcgatggt cgtcctcgcc gtgggcgaga
     2701 ggtaatgttc tggaccgtgc cgataactgt cacttcttcg ccgtaccaaa gccggttaat
     2761 cggttttagt tgcgtatagt caagatattt gcgggggaaa taatacatca tatcacgcaa
     2821 ggtatgcatt
//
LOCUS       DBNHOH010000991         3144 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1730369, whole genome shotgun sequence.
ACCESSION   DBNHOH010000991 DBNHOH010000000
VERSION     DBNHOH010000991.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1730369"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="AC16V7_17880"
     CDS             complement(<1..260)
                     /locus_tag="AC16V7_17880"
                     /inference="COORDINATES: protein motif:HMM:NF014049.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HIR6259050.1"
                     /translation="MTTQLIIGLTLGAIIGWLAWRAGSLSDSGAWAAALTGGLIFGLG
                     GIKWAVLLLAFFVSSSVWSRAFNRRKAHLSNQYAKGSRRDWG"
     gene            complement(272..1213)
                     /locus_tag="AC16V7_17885"
     CDS             complement(272..1213)
                     /locus_tag="AC16V7_17885"
                     /inference="COORDINATES: protein motif:HMM:NF022338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIR6259051.1"
                     /translation="MNTSFEFRPPRGAGVVFNLGGIVLLASGAIWGLWGASQAENNLV
                     FILYLIPVLVAVLGVPFLTYRVLALRGAFYRLERDGVTLRWGLRSEDIPIDAVLWVRP
                     AAEVGSGLPLPLLRWPGNVMGTRRLSGGGVVEFMAANTRQLVLIATSRLIFAVSPENP
                     AEFISIYHRQTELGSLTPLTPRTVYPTFLLGRVWQRRSARALLLTSLVLNLILVTWVA
                     LVAPVRGEVIMGVQRGGEVVPAVQLMLLPVLSSFFFLVDFFLGLFFYRRGVLLLATST
                     RVGSARNPNMVLAYLLWGSAVITSLMFFSAVAYILLS"
     gene            complement(1214..2446)
                     /locus_tag="AC16V7_17890"
     CDS             complement(1214..2446)
                     /locus_tag="AC16V7_17890"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR6259052.1"
                     /translation="MITLLHFADAHIDMANYGRHDPQSGLPLRVMDFLKSLDTIVDTA
                     IEEKVDLVLFAGDAYKDRSPAPTFQREWGKRIMRLSRAGIPILLLVGNHDLSPALGRA
                     HALESFDTLEVPRVRVLEKPEMLTPKDLWGLPLQILALPWISRSGIMAALDLSAADPK
                     QVYEQIEERLTGLIQDWLEAVDPSLPVVLTAHASVQGAIYGGERTVMLGSDLVLAGSL
                     VKDPRLDYVALGHIHKPQNINENAHPPVIYPGSIERVDFGEVKDDKFFVIARVGRGKT
                     EVEWRQLTGIRPFIERYLTLESQEGIAQKLSQALPPAEQIEGAIVRLVLEYPREWESL
                     IDESALREHTAAAFEFQLIKRPQMEARIRLPDGQAVGSLTPLELLDTYWDANHVQADE
                     KEALKNLAQNLIQEREEE"
     gene            complement(2451..>3144)
                     /locus_tag="AC16V7_17895"
     CDS             complement(2451..>3144)
                     /locus_tag="AC16V7_17895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR6259053.1"
                     /translation="YTGKLGWLGHYQFVTGYDEAKEVLIVQDTYVKDGKDHEFSYADF
                     IEGWRSFNYVFLVAFPRERAEEVYALLGPYADPTWAARHALEVAKAETETLTGMDQFF
                     AWFNVGTSHVNLQEYGDATYAFDYAFSTLYPNLPEDKLRPYRVMWYQTWPYWAYYYSG
                     RYQDVIDLANTTLYDTISEPVLEESFYWRGLAREALGDSQGAIDDFRTSVQLNPNFGP
                     GWEQLNRLGVGP"
BASE COUNT          748 a          958 c          839 g          599 t
ORIGIN      
        1 tgaccccaat cgcgccgcga gcctttggca tattggttag acaaatgagc tttacggcga
       61 ttgaaggctc gcgaccaaac gctggacgag acaaaaaacg ccagtaacag taccgcccac
      121 ttgataccgc ccaggccaaa gatcaaacca ccggtgagtg ccgctgccca cgctccactg
      181 tccgagagcg atcctgcgcg ccaagccagc cagccaatta tggcgcccaa cgttagacca
      241 atgattagct gggtggtcat actctaattg ttcaggatag aagaatgtaa gccactgcgc
      301 taaagaacat gagcgaggtg atcactgcgc tcccccatag caagtatgcc agcaccatat
      361 tggggttccg agctgaccca acccttgtgg aagtcgccag gagcaatact ccccggcgat
      421 aaaagaataa ccccaagaaa aaatcgacca gaaagaagaa actgctcagc acgggcagca
      481 acatcaactg aactgccggc acgacctccc caccgcgctg cacacccata atgacctcac
      541 cccttaccgg cgcaaccagg gctacccagg ttactaatat caagttgagt acgaggctag
      601 tcaacaacag tgcccgtgcc gagcgccgct gccagacacg ccccagcaga aatgtcggat
      661 atacggtgcg gggagtcagt ggggtgaggg agcccagctc ggtctggcga tggtagatcg
      721 atatgaattc cgccgggttt tctggcgaga cggcgaagat gaggcgcgag gtagcgatca
      781 acaccagttg ccgggtattg gctgccatga attccacgac acccccgcca ctcaggcggc
      841 gagtacccat gacatttccc ggccagcgca acaaaggcag tggcaagccg ctgcccacct
      901 cagccgctgg acgcacccac aacaccgcgt cgatggggat atcctctgag cgcaaccccc
      961 agcgcagcgt cacaccatcg cgttccaagc gataaaacgc cccgcgcaat gctaagacac
     1021 ggtaggtcaa aaaaggtaca cccaaaaccg caacaagcac cggtatcaaa taaagaataa
     1081 acaccaggtt attttccgcc tggctcgccc cccacagccc ccaaatggcg cctgaagcca
     1141 ggagcactat accgccgaga ttaaatacaa cccctgcgcc gcgtggaggg cggaattcga
     1201 aacttgtatt cacctactcc tcctcacgct cctgaatcag gttttgcgca agattcttga
     1261 gggcctcctt ctcatcggcc tgtacgtggt tggcatccca gtatgtatcg agcagttcca
     1321 atggagtcag gctacccacc gcctgtccat cgggcaggcg aatgcgcgcc tccatctgcg
     1381 ggcgcttgat cagctggaat tcaaacgcgg cagcggtatg ctcgcgcaaa gccgattcgt
     1441 cgatcaggct ctcccactca cgcgggtact caagcaccag gcgcacgata gccccttcga
     1501 tctgttcagc gggaggcagc gcctggctta atttctgagc aataccttct tgagattcca
     1561 gggttaagta gcgttcgata aaggggcgga ttccggtcag ttgccgccat tcgacctcgg
     1621 tcttgccgcg ccccacgcgg gcaataacga agaatttatc atccttcact tcgccgaaat
     1681 caacccgctc gatagagccg gggtagatca cgggcgggtg agcgttttcg ttaatgtttt
     1741 gcggcttgtg gatatgcccc agggcgacgt aatccaggcg agggtctttg accagagagc
     1801 cagcaagcac taagtcgctt cccaacatca ccgtgcgctc acctccatag atggctccct
     1861 gtaccgaagc gtgggcggtc agcacgaccg gcaatgaagg gtccacagcc tccagccagt
     1921 cttggatcaa accggtcagg cgctcctcga tctgctcata tacctgcttg gggtctgctg
     1981 cgctcaggtc tagcgcagcc atgatgcccg aacgggagat ccagggcagc gccaggattt
     2041 gcaggggaag cccccacaaa tccttaggag tcagcatttc tggtttttct aggactcgca
     2101 cgcgcggaac ttccagggtg tcgaagctct ccaaggcgtg ggcgcggccc agcgccgggg
     2161 acagatcatg gttgcccacc agcagcagga tgggaatacc cgcccgcgat aagcgcatga
     2221 tgcgctttcc ccactcgcgc tggaaagtgg gcgccgggct gcggtctttg taggcatcac
     2281 cggcgaagag caccagatcc actttctctt caatcgccgt atccacaatc gtatccagcg
     2341 acttaagaaa gtccatcacc cgcaaaggca aaccgctctg cgggtcgtgg cggccatagt
     2401 tagccatatc gatatgtgca tccgcgaagt gtaagagagt gatcatcgta tcagggtccc
     2461 acgcccaggc gattgagctg ttcccaaccc gggccaaaat tcgggttcaa ttgcaccgag
     2521 gtgcggaaat cgtcgatggc gccctggcta tcgccaagcg cctcacgcgc caggccgcgc
     2581 cagtaaaagc tctcttccaa taccggctcg ctgatcgtat catacagtgt ggtattcgcc
     2641 aggtcaatca catcttggta gcgacccgaa tagtagtacg cccaataagg ccaggtctgg
     2701 taccacatca cacggtatgg gcgcaactta tcttctggca ggtttggata cagggtggag
     2761 aaggcgtaat cgaaggcgta agtggcgtcg ccgtactcct gcaggttgac atggctggtg
     2821 cccacattga accaggcgaa gaactgatcc ataccagtca gagtctccgt ctcggcttta
     2881 gccacctcga gggcatggcg cgcagcccag gtcgggtcag catatgggcc cagcagagca
     2941 tacacctctt cggcacgctc cctcggaaag gccaccagga aaacatagtt aaacgatcgc
     3001 cagccttcga tgaaatcggc gtagctgaac tcgtgatcct tgccatcctt tacataggtg
     3061 tcctgcacga tcagcacttc ttttgcctca tcatagccgg tgacgaattg gtaatgcccc
     3121 aaccaaccca gcttgccggt gtaa
//
LOCUS       DBNHOH010000992         1517 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1409794, whole genome shotgun sequence.
ACCESSION   DBNHOH010000992 DBNHOH010000000
VERSION     DBNHOH010000992.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1409794"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..559)
                     /locus_tag="AC16V7_17900"
     CDS             complement(68..559)
                     /locus_tag="AC16V7_17900"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HIR6259054.1"
                     /translation="MIISIIVAMDEERGIGVNNRLPWHLSADLKRFKALTMGHHILMG
                     RKTYESIGRALPGRVMMVITRQQDYRPEGCLVVHSLQQGLALANSKGESEFFIIGGGE
                     IFAQSLDYADRIYLTLVHTVAGCDVFFPEININTWQERDASDQVADAQNDFPHTFKTL
                     FRR"
     gene            complement(556..1350)
                     /locus_tag="AC16V7_17905"
     CDS             complement(556..1350)
                     /locus_tag="AC16V7_17905"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013058746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HIR6259055.1"
                     /translation="MRQYLDLVQNILDHGVKKGDRTGTGTLSMFGYQMRFNLQDGFPL
                     LTTKKLHLKSIIYELIWFLNGDTNIKYLNDHGVRIWDEWADERGELGPVYGAQWRSWN
                     TGDGRSVDQISQVIKQIKTNPDSRRLIVSAWNVAEIENMALPPCHTLFQFYVANGRLS
                     CQLYQRSADVFLGVPFNIASYALLTMMIAQVCDLEAGDFVHTLGDAHLYLNHLEQARL
                     QLTRQPYPLPQMRINPAVKSIFDFEYADFELLNYQAHPSIKAEISV"
     gene            complement(1400..1513)
                     /locus_tag="AC16V7_17910"
     CDS             complement(1400..1513)
                     /locus_tag="AC16V7_17910"
                     /inference="COORDINATES: protein motif:HMM:NF013821.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HIR6259056.1"
                     /translation="MAHIRDEARFPNPQALIQQIHQDISRARSYLATQLNL"
BASE COUNT          402 a          392 c          328 g          395 t
ORIGIN      
        1 gcaggtatct tagcagaatg atctcgactt actttcaacc gacacaggca taacaaagca
       61 caaattctta tcgtctaaat aaagttttga atgtatgcgg gaaatcgttc tgcgcatcag
      121 caacttgatc agacgcatct ctttcctgcc aagtatttat attgatctcc ggaaaaaaca
      181 catcacaccc agcaactgta tggaccaggg ttagatatat cctatctgcg taatccagac
      241 tctgggcaaa gatttccccg ccgccgataa taaaaaactc actttccccc ttgctattag
      301 ccagcgctaa cccttgttgc agcgaatgga ccaccagaca gccctccggg cggtaatcct
      361 gctggcgagt gatcaccatc ataactctgc ctggtaatgc tcgaccaatc gactcatagg
      421 tcttgcgccc catcaggata tgatgcccca tagtcagtgc tttgaagcgc ttcaagtctg
      481 cagataaatg ccagggcaag cgattattta caccaatgcc gcgctcctca tccatcgcta
      541 cgatgattga gatgatcaca ccgagatttc cgctttaatg cttgggtgag cctggtaatt
      601 caacaactca aagtctgcat attcaaagtc aaatatggac ttaaccgcgg ggtttatgcg
      661 catctgcggg agcggatacg gttgtcgggt caattgcaaa cgcgcctgct ctaggtgatt
      721 caggtacagg tgcgcgtcac cgagtgtgtg cacaaaatct cccgcctcca gatcacatac
      781 ctgggcgatc atcatggtca aaagcgcata cgaggcaata ttaaaaggca cccccaaaaa
      841 gacatccgct gaacgctggt agagctggca ggatagccgt ccattggcaa cgtagaactg
      901 gaacagggta tggcaagggg gtaaggccat attttcaatc tccgccacgt tccaggcact
      961 gacaatcagc cggcgggagt ctggattggt cttgatttgc ttgatcacct gggatatttg
     1021 gtccacgctg cgcccatcgc ccgtgttcca cgaacgccat tgtgcaccat aaacaggtcc
     1081 cagctcgcca cgctcatcag cccattcgtc ccaaattcgc actccgtgat catttagata
     1141 ttttatgttt gtatctccat ttaaaaacca gattaattca taaatgatgg atttcaaatg
     1201 aagtttttta gtggtcaata acgggaaacc atcctggagg ttgaagcgca tctggtaacc
     1261 aaacatcgaa agtgtccctg tgcccgtgcg gtcccctttt ttcaccccgt gatccaatat
     1321 attttgcact agatcaaggt attgccgcat atattcctcc aattccctat ctatttacca
     1381 tcaattaagt agctaatatc tataaattta attgggttgc caaataactc cgcgcccggc
     1441 ttatgtcctg atgaatttgc tggatcaaag cttgcggatt tgggaaacgg gcttcatccc
     1501 taatgtgtgc caggaat
//
LOCUS       DBNHOH010000993         2344 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1153291, whole genome shotgun sequence.
ACCESSION   DBNHOH010000993 DBNHOH010000000
VERSION     DBNHOH010000993.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1153291"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1804
                     /locus_tag="AC16V7_17915"
     CDS             <1..1804
                     /locus_tag="AC16V7_17915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6259057.1"
                     /translation="YHCPHCNTQGEHPITEEDLARAAQFSTSSLHRARALERVAPLDD
                     PDRHHAEEALSVYLPRSVYALFNLINRLDGMSLAPQRRNHLTALLLSACDLGNALWPY
                     PSGRARPRALTIPPKFRENNLWFVLEQAIQHWSHPSDQVALSTWPEQPPHEGGISIFE
                     GRWRDLAAELADIPIEAVVTALPRPNHAFWTLSALWAGWLWGQEAVRPFKSVLRRRRY
                     DWNWHTTALHAAFENLPMILKPGSLFLGLIGEAEPGFLSAALVAADNANFELSGIAMP
                     TEGGQAQINWRLLSQVHDTPYSEEERVLRMRTAARDYLSTRGEPSHYLPIHTAVLSSL
                     AQIHALRSSEQAPMSMLVKINTAIDEALSYRGGLLRFGGSEKSMEVGYWWLRETDNIS
                     SALADRVEIELVQYLITHPGASLPDIYSAICHTFPGLLTPSAELIQICLESYGEETSP
                     GSGDWHIQAQNTPSARKTDLKDIASLIIQMGANLGYQVEGDQPILWRDAANQVAYAWH
                     VTASAVIGDAIFNQDYAPGISLVALPGGRANLVAFKLQHDPRLRQAFEEGWRFVKYRH
                     LRWLAQNPILSRDTLDHLLTQDTLTYESPQMRLF"
     gene            complement(1806..>2344)
                     /locus_tag="AC16V7_17920"
     CDS             complement(1806..>2344)
                     /locus_tag="AC16V7_17920"
                     /inference="COORDINATES: protein motif:HMM:NF024791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HIR6259058.1"
                     /translation="NLTLDQAVQLAIIASQVPQENIKRGVIGKGYVLFGQSPDNLSIL
                     IPLVDKIHTLRDEIFASSSALSPLTPGSELERMLAEGARVSIENQSRTTGAGLAERTS
                     QFLVNQGVNVASTSESPQNLTSTTLIDHTGKPFTARYLVNLIGISPGKIILDETPGSP
                     VDIEILLGDDWARENPIP"
BASE COUNT          544 a          641 c          570 g          589 t
ORIGIN      
        1 ctatcactgc ccacattgca atacccaagg tgagcatccc atcacggaag aagacctagc
       61 gcgggctgct cagttttcca ccagcagttt acatcgtgcc cgtgcactgg agcgtgttgc
      121 accgctcgat gatccagatc gccaccacgc agaggaagcc ctgtcagtct accttcctcg
      181 ctctgtgtac gctctgttta accttatcaa taggctcgac ggaatgagcc ttgcccctca
      241 gcgccgcaat catctaaccg ctctcttgct cagcgcatgc gacctgggaa acgccctttg
      301 gccctatcca tctgggcgcg cccggcctcg cgctttgacc attccgccga aatttcgcga
      361 gaacaactta tggtttgttc ttgagcaagc catccaacac tggtctcacc caagtgatca
      421 agttgcctta tccacctggc cagaacaacc accacatgaa ggaggtatct ccatctttga
      481 agggcgctgg cgagatctgg cggcggagct ggcagatatc cctattgagg ccgtggtaac
      541 tgccctaccc agacccaacc acgccttttg gacactctcc gccctatggg caggttggct
      601 atggggccaa gaggcagtca ggccattcaa gagcgttttg cgcaggcgcc gctatgattg
      661 gaactggcat acaaccgccc tgcacgccgc tttcgaaaat ctcccaatga ttttgaagcc
      721 gggatcacta ttcttaggat tgatcggcga ggctgagcct ggcttcctat ctgcggcatt
      781 ggttgcggca gataacgcaa attttgagct atcaggtatt gctatgccca ccgaaggtgg
      841 tcaagcacaa ataaattggc gactcttgag ccaagtccac gacactccgt actcagagga
      901 agaacgcgtt ttgcggatgc gcactgctgc aagagattat ttatccacac gaggtgaacc
      961 aagtcactac ctcccaatac acactgccgt cctgtcttct ttggcacaga ttcatgccct
     1021 acgttcgtct gaacaagctc cgatgagtat gttggtgaaa ataaatactg ccatagatga
     1081 ggcgctctct taccgtggag gtcttctacg ctttgggggc agtgaaaaat ccatggaagt
     1141 tggttattgg tggctacgcg aaacagataa tatttcttcg gcgcttgccg atcgtgtgga
     1201 gatagagctt gtgcaatatc tgattactca cccgggagct tcgcttcccg atatctattc
     1261 cgcaatatgc cataccttcc ctggactgct caccccaagt gctgagttga tccagatttg
     1321 cctggaatcc tatggtgaag agacctcacc cggaagcgga gattggcata tccaggctca
     1381 gaatacccct tcagcacgca agacagatct aaaagatatc gctagcctga tcatccaaat
     1441 gggcgctaat cttggatacc aggtagaagg tgatcaaccc attctttggc gggatgcggc
     1501 gaatcaagtc gcttacgcat ggcatgtcac cgcttcagca gtgattggtg atgcaatttt
     1561 caatcaggac tacgctcccg gaatttcttt agttgcctta cctggcgggc gcgcgaatct
     1621 ggtcgccttt aagcttcaac acgatcctcg cttgcgccag gcgttcgagg aagggtggcg
     1681 ttttgtcaaa tatcggcacc tgcgctggct ggcacagaac ccgatcttgt cgcgtgacac
     1741 cctggatcac ttgctcaccc aggatacatt gacctacgag tccccacaaa tgcggttgtt
     1801 ttaaactacg gtattgggtt ttcccgagcc caatcgtctc caagcaggat ctcaatatcc
     1861 actgggcttc ctggtgtttc atccaaaata atctttcctg gggaaatacc gatcaaattc
     1921 actagatacc tggcggtgaa tggcttacca gtatgatcga tcaaagtggt tgaagtcaaa
     1981 ttctggggcg attcgctcgt gctggctaca ttcacccctt ggtttaccag gaattgactg
     2041 gttcgttcag ccaagcccgc tcccgtcgta cggctttggt tttctattga gacgcgcgca
     2101 ccttctgcga gcatacgttc aagctctgaa cctggtgtta agggactaag cgcactcgat
     2161 gatgcaaaaa tctcatccct gagggtatga atcttatcaa caagtggtat caagatgctc
     2221 aggttatccg gtgactgacc gaaaagtacg taacctttac caatcactcc acgtttaatg
     2281 ttctcctgag gcacctggct ggcaatgata gctaattgga cagcttggtc tagtgttaaa
     2341 ttgg
//
LOCUS       DBNHOH010000994         2383 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_63386, whole genome shotgun sequence.
ACCESSION   DBNHOH010000994 DBNHOH010000000
VERSION     DBNHOH010000994.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_63386"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="AC16V7_17925"
     CDS             complement(<1..281)
                     /locus_tag="AC16V7_17925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR6259059.1"
                     /translation="MSAASRDFEKAALRGEPSYVWRAGQERRLKMILAAAGERVKGRV
                     LENGCGVGMYAAHLLPHAGSVTGLEYDFERASQASKVPGQVVNAAGERLP"
     gene            complement(492..1190)
                     /locus_tag="AC16V7_17930"
     CDS             complement(492..1190)
                     /locus_tag="AC16V7_17930"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HIR6259060.1"
                     /translation="MAYLTGGERARYVQGMFAHIARRYDLMNRLMTAGQDVRWRREVI
                     RRAELPASGRLLDLGAGTGDLAREALRQVPKSLPVAADFTLEMMRVGKTRPNMGLEWA
                     AADALCLPFPDGSFDAVVSGFLLRNVSDILQSLNEQRRVLKPGGRIIALDTTQPPKNP
                     LAPLIKLHLHTVSPTLGRLIAGSAEAYRYLPETTENFLSAEGLAERMQTAGFQGVGFR
                     RLMFGTVAIHWGFK"
     gene            1300..2268
                     /gene="gmd"
                     /locus_tag="AC16V7_17935"
     CDS             1300..2268
                     /gene="gmd"
                     /locus_tag="AC16V7_17935"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR6259061.1"
                     /translation="MPVALITGITGQDGSYLADFLLAKGYKVVGMVRRSSTVTFERIQ
                     HIQDDITVIQGDLHDQSSLVDVMEKYQPDEVYNLAAQSFVPTSWNQPVLTGEVTALGV
                     TRILEAIRHSNPKARFYQASSSEMFGKVREVPQRETTPFYPRSPYGVAKVYGHWITVN
                     YRESYDLYAVSGILFNHESPRRGLEFVTRKITYNAARIKLGLTKELRLGNLEARRDWG
                     FAGDYVEGMWLMLQQDEPADYVLGTGVTHSVREFCEAAFSYLGLDYNDYVIQDPRFYR
                     PAEVDLLVADPGNANEKLGWQQKVAFEDLVRLMVDADIKRIGVELP"
BASE COUNT          549 a          693 c          631 g          510 t
ORIGIN      
        1 ggcaaacgct ctcctgcagc gttcaccacc tggccaggga ctttgctcgc ctggctggca
       61 cgctcaaagt catattccag gccagtcact gatccagcat ggggcaacaa atgggcggca
      121 tacatgccca ccccacagcc attctccagc acgcgtccct taactcgctc accggccgcc
      181 gccaagatca tctttaggcg gcgctcttgc ccagctcgcc aaacgtatga aggctcccca
      241 cgcaaggcgg ctttctcaaa atcgcgagat gcggcactca aggcaggtac catccctgat
      301 tgaagttgaa gccagagcgg ttgcccatta gacgcgtcca gcgtgagata aagatgtaca
      361 gcgcgttgcg ctcgatttcc cctctaaact tggatacaaa gggaaatctg gcagtatgaa
      421 actcactgga ggaactggcg ttgggtagag acatggttaa gagggatttg gtcacattct
      481 gttttccggg atcacttgaa tccccagtgg attgccaccg tgccgaacat caggcggcgg
      541 aagcccaccc cctggaaacc agcggtctgc atgcgctccg caagcccttc agcgctcagg
      601 aaattctccg tggtttcggg taagtagcga taagcctcag cagacccagc aatcaggcga
      661 cccaaagtgg gactgacagt gtgcaggtgc agcttgatca aaggcgcaag agggtttttc
      721 ggtggttggg tggtatcaag tgcaattata cgtccaccgg gcttaagcac gcggcgctgc
      781 tcgttgagcg actgaaggat atcgcttaca ttacgcagca agaagcctga gacgactgca
      841 tcgaagctcc catccgggaa gggcaggcac agggcatcgg ctgctgccca ttccagcccc
      901 atattggggc gcgtcttgcc cactcgcatc atttccaagg tgaaatcggc tgctacgggc
      961 agactctttg gcacctggcg caacgcttca cgcgccagat cgcctgtgcc agcccccaga
     1021 tccagcaagc gaccactggc aggcaactcc gcccggcgga tcacctcgcg ccgccagcgc
     1081 acatcctgcc cggcggtcat caggcggttc atcaaatcat agcggcgcgc gatgtgtgca
     1141 aacataccct gcacataacg cgcccgctcg cctcctgtga gatatgccat cgtctctggg
     1201 atacctttcg gattctcact ataccatatt tgtacctctt gaccagcccc acccaacggt
     1261 tataattcac ccgttgaata ttatattgga gaaggcctta tgcctgttgc tctcatcaca
     1321 ggaataaccg gccaggatgg ctcatacctg gcggattttt tacttgccaa aggatacaag
     1381 gtcgttggca tggtacgccg ttccagcacc gtgacctttg agcgcatcca gcacatccaa
     1441 gatgatatca ccgtcattca gggagaccta cacgaccaga gctcgctggt cgacgtcatg
     1501 gagaagtacc agcccgatga agtgtacaat ctggccgcac aatcgtttgt gcctacctcc
     1561 tggaaccagc cggttcttac cggcgaggta accgcgctag gagtcacccg catactagaa
     1621 gccatacgcc attcaaatcc caaggcgcgt ttctaccagg cttcttctag cgaaatgttc
     1681 ggtaaggtgc gcgaagtgcc tcagcgggag accactccct tttacccgcg cagcccttac
     1741 ggcgtggcca aggtgtacgg gcactggatc acagtcaact atcgtgagtc atatgattta
     1801 tacgccgttt cgggcatttt attcaaccac gaaagcccgc gccgcggcct ggagtttgtc
     1861 acgcgcaaga tcacctacaa cgcggcccgt atcaaactgg gtctgacgaa agaactgcgc
     1921 ctgggcaatc tggaagcgcg acgagactgg ggcttcgctg gtgattacgt cgagggcatg
     1981 tggctgatgt tacagcagga cgaaccggct gattacgtgc tggggactgg cgtcacgcac
     2041 tcggtgcgcg aattctgtga agccgccttt agctatctag gattagacta caatgactac
     2101 gtaatccagg acccgcgctt ttaccgccca gccgaggtag acctgttggt agccgaccca
     2161 ggcaatgcca acgagaagct aggctggcaa caaaaggtgg ctttcgaaga cttggtgcgt
     2221 ttaatggtag atgcagacat aaagcggatt ggagtagaac tcccgtgacc aatcactcaa
     2281 tactgagacg gattgtcgct tctcgatggt cggcacatat cctttcgctt atagccttta
     2341 tattgtatct ggttcaagcg atttactatg ctcacaacca gga
//
LOCUS       DBNHOH010000995         1857 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_1153532, whole genome shotgun sequence.
ACCESSION   DBNHOH010000995 DBNHOH010000000
VERSION     DBNHOH010000995.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_1153532"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="AC16V7_17940"
     CDS             complement(<1..626)
                     /locus_tag="AC16V7_17940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6259062.1"
                     /translation="MSCSLFSQILVFLALIFTVFAFGRKLKIYAQLARPVDRAKPKGD
                     LQKGVLYAYTLGMAPWSKESTRLHSLAYLRGVAFHVGIFLGLAILVASPWLGEMPQYV
                     RNILGSVIAAGALLGLLGFVARFVEHNLKALSNADDYFAVLIVGLFLAAEALWLFFPA
                     ARVLFYLVSAAMLVYAPLGKIRHCIYFAYSRLFYGRFIGSRAVLPHSQ"
     gene            complement(637..876)
                     /locus_tag="AC16V7_17945"
     CDS             complement(637..876)
                     /locus_tag="AC16V7_17945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR6259063.1"
                     /translation="MSTQTTGMVLDLKGLLCPMPVIKLAKAIKDIPVGEIIEAFATDP
                     GVMADIPAWCRTTGNELVTLEKNDKQFRFVVRRVK"
     gene            complement(902..1435)
                     /locus_tag="AC16V7_17950"
     CDS             complement(902..1435)
                     /locus_tag="AC16V7_17950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003389587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HIR6259064.1"
                     /translation="MTVVDLPLANTLTVQSNEKSTKNKLSMVIFSGDMDKILAAFIIA
                     TGAAASGMEVTMFFTFWGLKAIQKSGVIAGKGLFGRMLGMMNRGGLDAIGPSRLNMGG
                     MGRWMFKLMMKQKGVASLPELYEMAVDLGVKMMPCQMSMDVMEIDPQLFQDNVEPPVG
                     VAAFIEEAAESKATLFI"
     gene            complement(1476..1814)
                     /locus_tag="AC16V7_17955"
     CDS             complement(1476..1814)
                     /locus_tag="AC16V7_17955"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR6259065.1"
                     /translation="MASPLEQEVNQLHAEICAGLADPKRILILYALAEKSCNVNELAS
                     YLNLSQPMVSRHLKVLRERGMVQANRVGVSVEYKLGDIRLIQALDLLRAVLHDDLSHR
                     AELVGALGEE"
BASE COUNT          513 a          466 c          403 g          475 t
ORIGIN      
        1 tgttgactat gaggcagcac tgctcggctt ccgataaatc taccgtaaaa cagacgcgaa
       61 tatgcaaaat agatacagtg gcggatttta cctaatgggg catataccaa catcgccgcg
      121 ctcaccagat aaaaaagcac tcgcgcggct gggaagaaca accatagggc ttcagccgcc
      181 aggaatagac cgacaatcag tactgcgaaa taatcatctg cattactcag tgctttcaga
      241 ttgtgctcca caaagcgagc gacaaaaccc aataaaccta atagagctcc agcagcaatg
      301 acagacccta gaatgttccg aacatactgg ggcatttcac cgagccaagg gctggcaacc
      361 aatattgcca gaccaagaaa aattccaacg tggaaggcca caccacgcag gtatgctaga
      421 gagtgaagcc gggtgctttc cttcgaccac ggcgccatac ccagggtgta ggcataaagg
      481 acaccctttt gtagatcacc tttaggcttt gcccgatcga ctggtcgcgc gagttgagca
      541 taaattttca gttttcgacc aaatgcgaat acggtaaaaa tgagcgccag aaaaacaagg
      601 atttgactaa atagagaaca cgacatttgc taggcctcat ttcacccgtc gaacaacaaa
      661 acgaaattgt ttatcatttt tttccagagt gactaattca ttccccgttg tgcggcacca
      721 agcgggtatg tctgccataa cacccggatc tgttgcgaat gcttcaatta tttcccctac
      781 aggaatatct ttgatcgcct ttgcaagttt aataacgggc atagggcaca gtagaccttt
      841 aagatccaat accataccag tggtttgtgt agacatttaa atattctcct aatttgaatt
      901 tttagataaa gagggttgct ttgctttcag cggcctcttc tatgaaagcc gccacaccca
      961 cgggcggttc aacattgtct tggaatagtt gtgggtcaat ctccatcaca tccatgctca
     1021 tttggcatgg catcattttg acccctaaat ctacagccat ttcatacagc tcgggtaaag
     1081 aggcaacacc tttttgcttc atcattaact tgaacatcca gcgtcccatc ccccccatat
     1141 tgaggcggct tgggccaata gcgtccaggc caccgcggtt catcattccg agcattcgtc
     1201 caaaaagacc tttcccagct attaccccag atttttggat cgccttcaat ccccagaatg
     1261 taaagaacat tgtgacttcc atacctgagg cggccgcacc ggtggcgata atgaacgcag
     1321 cgagtatttt atccatatcg ccgctaaaaa tcaccataga tagcttgttc ttggtgcttt
     1381 tttcattcga ttggactgtt aacgtgttag cgagagggag atctacgact gtcatgggtg
     1441 aggtaactcc tttgatatac caggtttaat tgagtttatt cttcaccaag cgcaccaact
     1501 aattcagccc gatgagatag gtcatcatgg aggaccgcac ggagcaggtc cagagcttgg
     1561 atcaggcgaa tatcgcccag cttatattcc acagatacac ctacccgatt ggcttgtacc
     1621 atcccgcgct cgcgcagtac tttgaggtgg cgcgagacca ttggctgact caggttcagg
     1681 taggatgcca actcattcac attgcaggac ttttcagcga gggcgtacaa aatcaatatg
     1741 cgtttagggt ctgccaatcc cgcacaaatt tctgcatgga gctgatttac ttcttgttct
     1801 agtggagatg ccatataatt cttctcctat atcatgcgca aatatacgga taagcat
//
LOCUS       DBNHOH010000996         3051 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_448519, whole genome shotgun sequence.
ACCESSION   DBNHOH010000996 DBNHOH010000000
VERSION     DBNHOH010000996.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_448519"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="AC16V7_17960"
     CDS             complement(<1..206)
                     /locus_tag="AC16V7_17960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6259066.1"
                     /translation="MKFRKTQILPILVSLILSICYPLFQPVSATQIQVDVLEQQPELA
                     SAPTPAPKGASQQNMALGPDQFPEG"
     gene            492..1145
                     /locus_tag="AC16V7_17965"
     CDS             492..1145
                     /locus_tag="AC16V7_17965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR6259067.1"
                     /translation="MKFHYILFISILLLTGSACNILSPETGSGGNPQPGAVLFQDDFS
                     DPSSGWDRITVNEGVTDYVDGTYRISVNSNFTDVWANPGLNFSDVRVEVDAIKVGGDD
                     NNDYGVICRYQDSENFYFFIVSSDGYYGIGKVVGGVQQLIGEESMPPSELINQGSVSN
                     RLSAVCAGSNLSLYVNGNFLTEVVDVDFTSGDVGLVAGTFDQPGVDVYFDNFMVTNP"
     gene            1201..2487
                     /gene="mtaB"
                     /locus_tag="AC16V7_17970"
     CDS             1201..2487
                     /gene="mtaB"
                     /locus_tag="AC16V7_17970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035598 - tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HIR6259068.1"
                     /translation="MKVYLDSVGCRLNQSEIEIYARQFRQAGYELVASPEGADLAVVN
                     TCAVTAQAASDSRQKIRQAARLGAQEVTVTGCWSTLNPRGALALPAVGRVVPNAQKDH
                     LVADLLQIPQETFELEPLARQLVPGARLRTRAFIKVQDGCDNRCAFCVTTLARGRGRS
                     RPISEVIADIQAALDGGSQEVVITGVHLGSWGADLSPDMRLGQLVKAILGETDVPRLR
                     LSSLEPWDVEADFFRLWENPRLCRHLHLPLQSGSAATLRRMARNTTPESFEALVDAAR
                     AAIPGIAITTDVIVGFPGESEDEFAESLDFIRRMRFSGGHVFTYSERHGTPAARMPDQ
                     VPHPLRKERNAYMRGAFSKAAAEYQALFLGSVLPVLWESAAALGPQGWRMSGLTDNYL
                     RVTALAEQHLWNRITPVRMTELVVDGLQGQVQEVGN"
     gene            2517..2861
                     /locus_tag="AC16V7_17975"
     CDS             2517..2861
                     /locus_tag="AC16V7_17975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018953834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HIR6259069.1"
                     /translation="MTDCIFCRIIAGEAPAEIVYTDEQATAFRDVRPVALTHILIVPN
                     KHIASVNDLTSDDEALVGHLFGLARQIAQQEGVDQTGYRLIVNTGQHAGQVVFHLHLH
                     LIGGQRMRFPMG"
     gene            2898..>3051
                     /locus_tag="AC16V7_17980"
     CDS             2898..>3051
                     /locus_tag="AC16V7_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017998205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HIR6259070.1"
                     /translation="MNTDMSEHHLPLAQIIQGDCVDVLNALPESSIDLIFADPPYNLQ
                     LNQELWR"
BASE COUNT          631 a          817 c          866 g          737 t
ORIGIN      
        1 ccctccggga actggtccgg gccaagggcc atgttttgtt gggaggcgcc tttgggcgcg
       61 ggcgtcggcg ctgatgccag ttccggttgc tgttccaaga catccacctg gatttgggtc
      121 gctgaaaccg gctggaatag cgggtagcaa atagagagta tgagagaaac caatataggc
      181 aggatctgtg tttttctaaa cttcattatc atcaattcct tactgaactt ctaaaacatg
      241 cacgctttgg ctggtactat acatcattaa tctgccggat gaagttaagc atttcatcaa
      301 gaacacttaa gaatcggtaa aatcgcccca aaattaatca attgggtgtg tttgctttcc
      361 agcagcccat atgtttcgga tcagaaaggc aatcggtcta tgcgattacc ccaggtacct
      421 gcgaaattcc ggcgcgggta tgttaaaatt gagcctctga aggcataaat cctttcgtca
      481 aggacacatt gatgaagttt cattatatcc tctttatttc tattctctta ctcacggggt
      541 ctgcttgcaa catactttcc cccgaaactg gcagcggagg taaccctcag cctggagcag
      601 tgctcttcca ggatgacttc tcagacccgt ccagcggctg ggaccgcatc actgtcaatg
      661 agggtgtcac cgattatgtc gatggcacct accgtatttc tgtcaattcc aactttacgg
      721 acgtttgggc aaaccccggc ctgaatttca gcgatgtgcg tgttgaggta gatgccatca
      781 aagtgggggg ggatgacaat aacgattacg gtgtgatctg ccgctaccag gacagcgaga
      841 atttttactt ctttattgtc agcagcgatg gttactatgg cattgggaaa gttgtgggag
      901 gcgtacagca gctgatcggt gaggagtcca tgccacccag cgagctgatc aaccagggta
      961 gtgtcagcaa tcgtctgagc gcggtttgtg cgggcagcaa tctcagcctg tatgtcaatg
     1021 gaaatttttt gaccgaggtt gtagacgttg atttcacctc tggggacgtg ggtttggtcg
     1081 caggcacctt cgaccaaccc ggtgtggatg tctactttga taattttatg gtgacaaatc
     1141 cataaacttt cccatgatcc cttgtcccat ggcgataagg gatacaggat tttgtctgcg
     1201 atgaaggtat atctagattc cgttggctgc cgcctgaacc agagcgaaat tgaaatctat
     1261 gcccgccagt tccgacaggc cgggtatgag ctagtcgcct cgccggaggg agcagatttg
     1321 gcagtggtca atacctgtgc ggtgactgcc caggcggctt ccgattcgcg ccagaagatc
     1381 cgccaggcgg ctcgtctcgg cgctcaagag gtcacggtca ctggttgttg gtccacgctc
     1441 aatccgcggg gtgcgctggc tctgccagcc gtcggccgcg tggtgcccaa tgcgcaaaaa
     1501 gaccacctgg tggcggactt gctgcaaatc ccacaagaga cctttgaact ggaaccgctc
     1561 gcccgccaac tggtgcctgg cgcccggctg cgcacgcgcg ctttcatcaa ggtgcaagat
     1621 ggctgtgaca accggtgtgc cttctgcgtg accactctag cccgcggccg tgggcgcagt
     1681 cgccccatct ccgaggtgat tgccgatatc caggcagccc tggatggtgg cagccaagag
     1741 gtggtgatca ccggtgttca cctgggctct tggggtgcag acctctcgcc ggacatgcgc
     1801 ttgggtcagc tggtgaaagc tattttgggt gagacggatg tcccccgtct gcgcctgtca
     1861 tccctggaac catgggatgt ggaagcagat ttcttccgtc tgtgggaaaa cccgcggctg
     1921 tgccgccacc tgcacctgcc gctccaatcg ggcagtgcgg ccacgctgcg ccgcatggcg
     1981 cgaaatacca ctccggaatc atttgaagcc ttggtcgatg cggcgcgggc agccatcccg
     2041 gggattgcga tcaccacgga cgtgatcgtt ggcttcccag gcgagagcga agatgagttt
     2101 gctgagagcc tggacttcat tcgccgcatg cgcttctctg gagggcacgt tttcacctat
     2161 tctgagcggc atggcacccc cgcggcgcgc atgcctgacc aggttcccca cccgctgcgc
     2221 aaggagcgca acgcttatat gcggggcgcc ttctccaaag cggctgcaga ataccaggcg
     2281 ctctttctag gcagcgttct gccggtcttg tgggagagcg ctgctgcgct aggcccgcag
     2341 ggttggcgca tgagcgggct gaccgataac tacctgcgcg taactgccct cgctgagcag
     2401 catctgtgga accgcatcac accagtacgc atgaccgaac tggttgtcga tgggttgcag
     2461 gggcaggtgc aggaagttgg taattaggtt ttttggcgaa aaacatggag tgatttttga
     2521 ctgattgcat cttttgtcgt attattgccg gcgaggcgcc ggctgagatc gtctacacgg
     2581 acgaacaagc cacagccttt cgcgatgtcc gcccggtggc gctcacccac atccttattg
     2641 ttcccaataa acacattgcc tcggtcaacg atctgacctc cgatgatgag gcgctggtag
     2701 gtcacttgtt tggcctagct cgccagattg cccaacaaga gggcgttgac caaaccggct
     2761 accgcctgat cgtcaacaca gggcagcatg ctgggcaggt ggttttccat ctgcacctgc
     2821 acttaatcgg tgggcagaga atgcgcttcc ccatgggtta gccgcttgtt ttcggtcttt
     2881 cctctatatc ggttaaaatg aatactgaca tgagcgagca tcatctgcct ttggctcaga
     2941 tcatccaggg ggattgcgtc gatgttctca atgctttacc tgagagctcg attgatctga
     3001 tctttgcaga cccaccctat aacttgcagc tgaaccagga gctgtggcga c
//
LOCUS       DBNHOH010000997         1845 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_192155, whole genome shotgun sequence.
ACCESSION   DBNHOH010000997 DBNHOH010000000
VERSION     DBNHOH010000997.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_192155"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1355)
                     /gene="nusA"
                     /locus_tag="AC16V7_17985"
     CDS             complement(<1..1355)
                     /gene="nusA"
                     /locus_tag="AC16V7_17985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR6259071.1"
                     /translation="MKNEFVLAFNEVLEEKQLPRDVILEALQAAMVSAYRRAVNASNA
                     QQIEATVDLETGKVSIFAEKEVVDVVQDERTEVALEDARRVDPEAEFGGMVIVDSTPD
                     NFGRVAAQTARQVIQQRIREAERDAQMQFFGKQLGEIVSGVVQSVNSQAITIGLDMKA
                     EGIMPRNQQIPGERFRVHDRVRALLMEVKPTSRGPQIILSRAHRNYLRRLLENEVPEI
                     YQGLVEIRSIAREPGQRSKVAVSALQPGVDPVGACVGMRGVRIQAIVRELNDEKIDVI
                     EWSSDPTIYIAKALSPARVNGVYLNELAKGAKTATVVVPEDQLSLAIGRDGQNARLAA
                     KLTSWRIDIKSLPEAATDALAKLQRDPEYAFMLEQEGEVFPQVELILAKKSEGRPVTP
                     EEYTVLNQFVDRVEHGLLLKREKELKAEQERLRVAEANVPSAAYDIPLEDLGISLRFS
                     T"
     gene            complement(1531..>1845)
                     /locus_tag="AC16V7_17990"
     CDS             complement(1531..>1845)
                     /locus_tag="AC16V7_17990"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR6259072.1"
                     /translation="SRKELADGFQRAYIYGFRMKSEYRGYGLGTYMLKYVEIDLVNRG
                     FQIAVLNVARENEDARLLYERQGYQVVAAEPGQWSYLDENGNRRWVNEPAWRMEKILD
                     SN"
BASE COUNT          434 a          531 c          406 g          474 t
ORIGIN      
        1 attgtagaaa agcgcaatga aatacccaag tcttcgagcg gtatatcata tgcagcactg
       61 ggcacattgg cttcagctac gcgcagccgc tcttgctcag ccttcagttc cttctctcgc
      121 ttgagtagca ggccgtgttc tacccgatca acaaactggt tgagcactgt gtactcttca
      181 ggagtaactg ggcgtccttc agacttcttc gcaaggatca attcaacctg agggaacact
      241 tccccttctt gctccaacat gaatgcgtat tctggatcgc gttgcagttt agccaatgca
      301 tccgtggccg cctccggcag gctcttaata tcgatacgcc aggaagtcag cttggctgct
      361 agccgagcat tctgaccatc ccgcccgatt gctaaactaa gctggtcctc agggactaca
      421 actgtagctg tctttgcccc tttggcaagt tcattcagat atacaccatt tacgcgcgct
      481 gggctgagcg ccttggcgat ataaattgta ggatctgaac tccactcgat aacatcgatc
      541 ttctcatcat tcaattcccg gacgatggcc tgaatacgca cacctcgcat gccaacacag
      601 gccccaaccg gatcaacacc tggctgcaaa gccgatacag caacttttga tcgttgccct
      661 ggctcgcgag caatggagcg aatttcgact agtccttgat agatttcagg gacttcgttc
      721 tccaaaagac ggcgtaaata attgcgatga gcgcgggaaa ggatgatctg agggccgcgc
      781 gaggtgggtt taacctccat cagcaatgcc ctcacccggt catgcacccg gaaccgctct
      841 ccaggtattt gctggttgcg cggcataatc ccttcggcct tcatatccaa accaatcgtg
      901 atggcctggg aattgactga ctgcactact ccgctgacga tctctccgag ctgtttgcca
      961 aaaaactgca tctgggcatc acgttcagcc tcacgtatgc gttgctggat cacctggcga
     1021 gccgtctgcg cagctaccct tccaaaattg tctggggtcg aatcgacgat caccatccca
     1081 ccgaattccg cctcggggtc tacccgacgc gcgtcttcca atgcaacctc ggtgcgctca
     1141 tcttggacaa catctacgac ctctttttca gcgaaaatag agaccttgcc agtttccaaa
     1201 tccacggtgg cttcaatttg ctgtgcgttg gaagcgttaa ccgcccgccg ataagccgat
     1261 accatcgcag cctgtagcgc ttccaggata acatctctgg gaagctgttt ttcttccaac
     1321 acttcgttga aagccaggac aaactcgttc ttcataactt accttatctc ctgtgcttga
     1381 tcctttaggc agcaactctt tattatatgc agaaaagtgg gggttgccca cttttcacca
     1441 gttcatatca acgtcgcttg cctgccctgg caatatttta gcacgaattc gtgaggcaga
     1501 caaggttaag tcaacccttc ttataggatt ttaattcgaa tcgagtattt tttccatccg
     1561 ccaggcaggc tcatttaccc agcgccggtt tccattctcg tcgagataag accattgccc
     1621 tggctcggct gcaacaacct gatacccctg acgctcatat agtagacgag catcctcatt
     1681 ttctcgagcc acattgagca cagcgatttg gaagccacga ttcaccagat caatctctac
     1741 atatttaagc atataggtac ccagcccata gccacgatat tcggacttca ttcgaaatcc
     1801 atagatatat gcgcgctgaa atccatcggc aagctctttg cgaga
//
LOCUS       DBNHOH010000998         1659 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate MAG04920
            SRR25158341_k127_192223, whole genome shotgun sequence.
ACCESSION   DBNHOH010000998 DBNHOH010000000
VERSION     DBNHOH010000998.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414328
            Sequence Read Archive: SRR25158341
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 05:18:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,600
            CDSs (total)                      :: 3,565
            Genes (coding)                    :: 3,547
            CDSs (with protein)               :: 3,547
            Genes (RNA)                       :: 35
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 18
            CDSs (without protein)            :: 18
            Pseudo Genes (ambiguous residues) :: 0 of 18
            Pseudo Genes (frameshifted)       :: 1 of 18
            Pseudo Genes (incomplete)         :: 13 of 18
            Pseudo Genes (internal stop)      :: 4 of 18
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158341_k127_192223"
                     /isolate="MAG04920"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.59 N 121.14 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1036
                     /locus_tag="AC16V7_17995"
     CDS             161..1036
                     /locus_tag="AC16V7_17995"
                     /inference="COORDINATES: protein motif:HMM:NF021592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HIR6259073.1"
                     /translation="MPPTLAYPLSAFLMIALPLGLGIALERKFRLGWRLWWIGGAGFV
                     LSQVGHIPFNIGLTVLFQRGILPAPPDSAKLVFNSTILGLSAGLWEELTRYAVFRWWV
                     KDARSWRKGVWLGAGHGGIEAVLLGIIVFANFFYQMAIRNVDLSTMVPQNQLAIAKDA
                     VAAYWSAPWYLILLGALERAMTLPIQICFSVLILQSFIRGQIGWLFLAVGWHAIIDSV
                     VVFSASTRGVYIAEFLVAVACLISLTIIFVLRKPEPQPMEDSTVDAFQPEPPQVIDHN
                     IIETPEKIDRTRFIE"
     gene            1033..>1659
                     /locus_tag="AC16V7_18000"
     CDS             1033..>1659
                     /locus_tag="AC16V7_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014406828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR6259074.1"
                     /translation="MIQTHNLTKEFNGTLAVERLNLEVGEGEVFGFLGPNGAGKTTTI
                     RMLTSLIAPTSGGAIVNGYTIGEDDRKIRQTVGILTESPGLYDSLSAEYNLQIYANLY
                     EVKDVRGQVEKYLRMLGLWDRRQDEVGTFSKGMKQKLAIARAILHEPRILFLDEPTAG
                     LDPEASHLVREFIEELRKQGRTIFMCTHNLDEADRLCDKIGVFKTRLLT"
BASE COUNT          396 a          429 c          423 g          411 t
ORIGIN      
        1 ttgcgcatgc ccctcaggtc atatgcaatt gccctgcgac gatcagcgag tctatttagt
       61 ttataaataa atcggcaggt ataataatct tgacctcacc cctatttcat agatgtacta
      121 atgccccatg tgggggggac gaaaaggtta ctacacatcc atgcctccta ccctcgcata
      181 cccgctatct gcttttctga tgatcgctct gccccttggt ttggggatag ccttggagcg
      241 taaattcaga ttgggatggc gactttggtg gatcggtgga gctgggttcg ttctttcgca
      301 agtgggtcat atccccttca atatcgggct gactgtccta tttcaaagag gcatactccc
      361 cgccccaccc gactccgcca agctggtttt caactcgaca atcttgggat tgagcgctgg
      421 cttatgggaa gaacttacac gctacgccgt ttttcgctgg tgggtaaaag atgcacgctc
      481 gtggcgcaag ggagtctggc tgggcgcggg gcatggtggg atcgaggcgg ttctgctggg
      541 tattattgta tttgccaact tcttctatca aatggctata cgcaacgtag acctgtccac
      601 catggtgcct caaaatcaac ttgccatcgc caaggatgca gtcgctgcct actggtctgc
      661 gccttggtat cttatcctgt tgggagctct ggagcgggct atgacactgc ctatccagat
      721 ctgcttcagc gtgctcatct tgcaatcctt tattcgcggg cagattggat ggctcttcct
      781 ggccgtgggc tggcacgcca taatagacag cgtggtagtc ttcagcgcga gtacgcgggg
      841 tgtatatata gctgaattcc ttgtcgccgt tgcctgtctc atcagcttga ctatcatatt
      901 tgtactgcga aaaccagagc cgcaacccat ggaagattcc actgtcgatg cttttcaacc
      961 cgagccccca caagttattg accataatat aattgaaaca ccggaaaaaa ttgaccggac
     1021 acggtttata gaatgattca aacgcacaat ctgaccaaag aatttaacgg cacgcttgcc
     1081 gttgagcgcc taaacttgga agtaggtgaa ggagaagtat ttggcttcct tggacccaat
     1141 ggagcaggga agactaccac catccgcatg ctgaccagcc tgatcgcccc aaccagcgga
     1201 ggcgccattg tcaacggcta caccatcgga gaagacgacc ggaaaattcg ccaaacggta
     1261 ggtattctca ccgaatcgcc tggtttgtac gacagcctga gcgccgaata caatcttcag
     1321 atttatgcaa atctgtatga agtgaaggat gtgcgcggcc aggtggagaa atacctgcgg
     1381 atgctaggcc tgtgggacag acgccaggat gaggtgggta cattctcgaa aggcatgaag
     1441 cagaaattgg caattgcccg tgccatactc cacgaaccgc gtatattgtt tctggatgaa
     1501 cccacagccg gcctcgaccc tgaagcctcc cacctggtgc gcgagtttat tgaagagctg
     1561 cgcaaacaag gtcgcacgat cttcatgtgc acgcacaacc tcgacgaagc cgatcgtctg
     1621 tgtgacaaaa ttggcgtatt taaaacccga ttattaacc
//
